LOCUS       DATNDT010000001        30393 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4311926, whole genome shotgun sequence.
ACCESSION   DATNDT010000001 DATNDT010000000
VERSION     DATNDT010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 30393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30393
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4311926"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..294)
                     /locus_tag="VN788_00005"
     CDS             complement(31..294)
                     /locus_tag="VN788_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18942.1"
                     /translation="MRWPATGDEIVAEGWVATGEVAWCVCAPVFAYQRGAEKMWVQAV
                     TVEWPAQFIEHTTETCPKAQPEEKPADKPPEKLVDENGQHRMF"
     gene            complement(269..625)
                     /locus_tag="VN788_00010"
     CDS             complement(269..625)
                     /locus_tag="VN788_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18943.1"
                     /translation="MRSMGYSISCGHVLVIMVELLRDGLLDRGGIEEIFMDFDGYTAA
                     EVLDMSENLFSAPARDFLDERSARILAQGIKRWFAKHPMTEKNSSPLQVVMQFFLDSM
                     WRCRKEENFAVAGHGR"
     gene            complement(836..2107)
                     /locus_tag="VN788_00015"
     CDS             complement(836..2107)
                     /locus_tag="VN788_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18944.1"
                     /translation="MAAQPESIWHDPAFDAARMRHWRPKNLRKARERAEENEPYVCEE
                     REHDEQITRRHDRENPKPSGNPDRAIPRFFDDAGELPSWLVRAIPESQNVQAKLLFHA
                     GMIGKGNRQALCGAVARRIDCVEHDEHRYFRKFQCQNRYCPACGPVCFRELFARHVDL
                     RGCVERLRAGEKSIGWRKVESGGKCKRERIWQASLTGTVRTCVLAKLDITTKKLGRMP
                     TKDEVRRFNEDIRRFFRAVESEFGISRGEYGALWCCEFGRMNENLHAHGIYCGPWLKQ
                     KKLSRLWRETRADGSFIVSIKIAKSFEQALAHCLKYPSKFFDAPAERLASLEKAFHRV
                     RRVHAVACFYNFHEEREPGEDTGSDTGLCPECKGMLGEPYSKEDRGWSLTSRLCGEGR
                     RDLDTVKASARERMSEFEASAGLVFGDGASP"
     gene            complement(2091..2306)
                     /locus_tag="VN788_00020"
     CDS             complement(2091..2306)
                     /locus_tag="VN788_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18945.1"
                     /translation="MHSKNPNYVTLRGAEDEYGVERHTLREWLERDLGLVFPEVVRGS
                     KFLISRGDVMRCIELHQPRAVGDGSAT"
     gene            complement(2287..3165)
                     /locus_tag="VN788_00025"
     CDS             complement(2287..3165)
                     /locus_tag="VN788_00025"
                     /inference="COORDINATES: protein motif:HMM:NF024965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HXU18946.1"
                     /translation="MKEKFKIHRWDTRVVIAEVEAESLKEGIEKLVAQDVNLDCARLV
                     GASLDGARLDGASLDGARLDGARLDGARLDGASLDGARLDGASLDGARLDGARLDGAS
                     LDCASLDGASLDGASLVGASLDGASLDGASLDGARLDCARLDGASLVGARLDCARLDG
                     ASLVGARLDGASLVGARLDGASLEFFKNDLWNVLLHVPHEIHGLALALKEGRVDGSVY
                     QGECACLVGTIANLRGCNYSQLPDLRPDSSRPAEQWFMSIRKGDTAESSVVVKLTLEW
                     IQDFYNLIGQYAQQKS"
     gene            complement(3266..3649)
                     /locus_tag="VN788_00030"
     CDS             complement(3266..3649)
                     /locus_tag="VN788_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18947.1"
                     /translation="MKTVDISGMDPELKRYGYEWGCQIIMFRALRWLREHPGSYPRIK
                     SFRSIVGVTVPNNQAAREMEDYALNHDKLREFGATGAMVQFAISHAMRRNVDEKSYFA
                     SLDAAGRRVFDFDERDAFPEEQNGA"
     gene            complement(3869..4243)
                     /locus_tag="VN788_00035"
     CDS             complement(3869..4243)
                     /locus_tag="VN788_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18948.1"
                     /translation="MTQFRGRTEDASAPFLRADFWEKGRELSGTVIRTFKTENGLAIV
                     LRTVSPVMLEGKSEKNISVGASAGLRMAVDAAGVPGGFSGLLIGDQIHLRSTGTTETD
                     KGNDQVNFEVEVNRPDAAAARA"
     gene            complement(4694..6298)
                     /locus_tag="VN788_00040"
     CDS             complement(4694..6298)
                     /locus_tag="VN788_00040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXU18949.1"
                     /translation="MSTAVMRGAVPAPPLQLEGSFTTIELRFLRESKTNPRRHFAGVE
                     ELAASVKQHGVLTPLLVRRTDGGELTYEIIAGARRYRAAKSAGIAAVPCRVLALTDEQ
                     ALEFQVIENLQREDVHPLDEALGYQALLRDGRYDVPSIAAKVSKSESYVYQRLKLAAL
                     IPAAQKMFFSDEITAGHAILIARLQPREQEKLLADIRRNGDDGIGGVRALARRIHEDF
                     HLELRNAPWDLADEALLPAAGSCVKCPKRTGANPQLFPDVKAKDICTDPACYRKKLEA
                     QVEQNTKNGLVSISSGYREYGEKGVPGVLYQGNRGYTFADKKCPSSKDAVVVLSGGYH
                     GDAKVGIEKRICADAKCKVHHPYGAEDVSQFSGTSPKQKAADRKQRAIAKVRTLALGK
                     IAEAKLSFNVCAGVIAEALLGRLHHDAAKLLANGLGIVPAKGKVVGFDYREAIEKRVA
                     EIQKAGNFARRIVPLLAAAAATPAFGGWNNDYHGEDKERLQRVAKAAHVPLAKMESGA
                     LAAIKSKKSASKAKVQTSARKGKKRK"
     gene            complement(6370..6555)
                     /locus_tag="VN788_00045"
     CDS             complement(6370..6555)
                     /locus_tag="VN788_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18950.1"
                     /translation="MDERDEQQLQEARDKAKGEDAFFLGEIVLQVARMNQHLAELNGH
                     IEVFLHKIDRVSTIFGK"
     gene            6644..6943
                     /locus_tag="VN788_00050"
     CDS             6644..6943
                     /locus_tag="VN788_00050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18951.1"
                     /translation="MNMPFAYIMSCSEPSLGEFSLAVLNRLAEAKAARRDLEEQIDEL
                     EGTAQLIEWLRARPGARARLLNLELPQGVFSFMREEIKPAALPPRREERAMRLAG"
     gene            complement(7286..7729)
                     /locus_tag="VN788_00055"
     CDS             complement(7286..7729)
                     /locus_tag="VN788_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HXU18952.1"
                     /translation="MPLQLSSSQAVEFSMLVLVTGREARGDGLDSMRAVAWSIRNRVS
                     RPSWWGRSWIEVITKKWQYSSINGLEADPNLLIYPVPGAAVWNDAISAAEEAYSGEGT
                     DPTGGAVSYYDRSLDDFPPLWAKSGQFAKTVDIGRLHFFRLVLPN"
     gene            complement(7875..8405)
                     /locus_tag="VN788_00060"
     CDS             complement(7875..8405)
                     /locus_tag="VN788_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18953.1"
                     /translation="MPARSTFAAAIAYAEGYGPPAARPTRNNNPGDLKNWPGVSTDAG
                     GYSVFPSAQAGWNALEQQLATIAAGQSSYYSPEMTLAQMGEIWAGGDSSWASNVASYL
                     GVSTAAMIGTFLGQGVQGVQGVPGSTDSFVVGTSAPLPAAPAAVAPPASPDMAALAMI
                     LGGVLAVYLVARDLLP"
     gene            complement(8406..8717)
                     /locus_tag="VN788_00065"
     CDS             complement(8406..8717)
                     /locus_tag="VN788_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18954.1"
                     /translation="MAVEMWSYIVDPGDGTIKMAHVFYGKTEKEARTYYDEHLSHCKY
                     MQGANSGAGEGQLIDDIVEVDELPSPEDFEDDDDDLDEDEREEIEEDEDDEIGDDDDE
                     E"
     gene            complement(8707..8943)
                     /locus_tag="VN788_00070"
     CDS             complement(8707..8943)
                     /locus_tag="VN788_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18955.1"
                     /translation="MARMHTLHIFGIVAWAALAALVIYCASQLVRALLAGMKAGFQEL
                     RRHREEEITLREKELRHRRQEEIVREYWEARRGR"
     gene            complement(8977..9288)
                     /locus_tag="VN788_00075"
     CDS             complement(8977..9288)
                     /locus_tag="VN788_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18956.1"
                     /translation="MSNVLDSVLGNLSKANTIVTLGVEVAGEIVPLIKGTVKKIEQLS
                     TGENTVTYSVLVQTDVQALADVESLSEADLEAINGELARLGKPPLPGPPARQPNPPSA
                     T"
     gene            9385..9615
                     /locus_tag="VN788_00080"
     CDS             9385..9615
                     /locus_tag="VN788_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18957.1"
                     /translation="MEQKPRISASHKSAKRIREHIIVSVSSRTRRKCQYPPCGKSYHP
                     KRYWQRFCSDKCKADDWRSHHPMTYEPKPFKD"
     gene            complement(9612..10136)
                     /locus_tag="VN788_00085"
     CDS             complement(9612..10136)
                     /locus_tag="VN788_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18958.1"
                     /translation="MSIASFRRQLKTKIVFGFILLFASLGYMGCAARVKNVTGAPAGV
                     SVQQVQQWDAAVANLQKIATVTSSLRQAVMSLNRSGAFPDGDAYTQTLVGIARIDTAQ
                     IQASRFLESVPDDWTQPTQQRVQAYISQVQQALTGLTSSGAAGIKDPNRHKEVSALVA
                     QLAADASAIVSLFG"
     gene            complement(10158..12812)
                     /locus_tag="VN788_00090"
     CDS             complement(10158..12812)
                     /locus_tag="VN788_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18959.1"
                     /translation="MQLARRLWIGALLGALLSVFAAGAQAQTFDADGGWQQSPCSGGS
                     KGFFYTEKAGNRWWFCTPEGNHFFANALANMQVPGINASVIWASGVLAKYGNSTQTAA
                     KDTQSKYLRSYGYNVAAEDSQAMYLPWSSTLCTGCLVTPGFTNTNTSVYATVNLNGAA
                     SHPVKNYIIGVNDYFTAFTGAGCADVFDPQYQVFATNNLAPQHNFLNAHNLGLTVDDT
                     DFQCGMGGSDAFDTFPAGHSKANLAMVVMQTSPVETLNNGGNRTYQGKVILYADTNVY
                     SKHLSATPPTSLSQCWSLTSITMPRCSLPDYLNAEYGGSISALNTAWGGATYTTFGSS
                     GTTISAFNCGTGNGSQILFTCTLPTAAGNISPLSVAVFENSTEIGGDCAWWENNCAHI
                     SNPSTPYNCATAGTCQGVILGDATSTLASGQQAFLSDNPCGAVGANPSAPAASFWAIV
                     LWHGSGTYIASRLTGETCAAGKGDSVLAPVSPPAGVTGYDVYMACRLSSGSTPSFGCV
                     ASTASQPAPTLQATNVSLSTNFIVSSTGLISGAAVPGPQSFLDYGTGAVQLTFSTAPA
                     TGAAITINAIAGGWMWGTGLMDEDGRHAWLGGGVNSICLVNGGGATPGTPGYSCRAGN
                     PGNNPTTFMNATAGQDLSNWQYQDAAQYFSAVRTALKTTFPNLLYFGPNTLGDWNTPA
                     HSQILQAAGVYLDVLFSSIYNTFPTDEMQRLSFEQQYFGDHPIFDEIFLQAAADSAVS
                     SCTSCQSSPGAYATQAARGTAEYNLKNLCLNTLGFNNDFQCVGLDHWGMADFNASEVN
                     NWGWTTPSDNDYNGIEPSAGAVPCVVNPSLTCGGEPTPAAPAVLPFGNGLTGATGVVA
                     ANKLWFQGGVVPVPIAPSKLIVTVSGEK"
     gene            complement(12803..14746)
                     /locus_tag="VN788_00095"
     CDS             complement(12803..14746)
                     /locus_tag="VN788_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18960.1"
                     /translation="MKRLICLLIILFGVARIAAAQSQTTVTATVVDPNGVPYGDATLK
                     ATLSPPGVQSPCVFTGSNCVPIQGTVGPVSLDSTGSFTLNLYPNASITPALTQWTFTV
                     CISPGVVPPLGTGPQCFSFTATIAGLSQDLSATLSAAAPALAHITGGGGGGFTAGGDL
                     SGTGISQNVIGFHFGATRIPLSATAPSTGQSLGFNGTSIVGLPSLLGLNNTWTALNEF
                     QQVLTVDDDIVSQNTIPATAGANSSSTAIGVLGNFFNGAASEPEDFSMQMVFGTGNNP
                     SATANFVFNFRNLNVGTTGALGVSYSSINPTSPVFLSASGPVNSAQGFSIGNAAPLNH
                     CLVGNGTLYTDAACPGGGFANPMTTAGDMIAGAAAGVATRVPGGLTGQTIVAQNATLP
                     VFASPGLAWGNAGAAVASTPYAVLCDSGTAVQDRSTTIDFQTGASVINLPDPTAAGCA
                     GNFVVSLYDDGAGTLTVNRGGAATLNVLDGLTDTQGVTSFTLSAGQTATLNANAAGTI
                     WLVKISKRTNLGTLYSWNAQSQTVAASSTLFGPLSGSSSGNASSTTRAGPITVACTAG
                     TGEAVTNSAQSALNSLVLTLFDVTTTVATGFSITIPAGAASGKFNSAGSATITAGDLY
                     AWQMVNNAAATSAQIIGVGFQCN"
     gene            complement(14749..16692)
                     /locus_tag="VN788_00100"
     CDS             complement(14749..16692)
                     /locus_tag="VN788_00100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18961.1"
                     /translation="MTWRRRLFVFLWLLELALFWAPPRAAAQSQTTVTGNVVDPKGFF
                     YAGGSVKATLFPPGVASPCVFTGPNCVPIPGTVGTVPIDFFGNFTINLYPNNLIFCNQ
                     GPCTTQWTFQICIQPGVIEPPIGTGDQCFAVTTTISGASQNITAQITAAPPPQLTRIP
                     IGGGGGGCTPSGVDTGILSEHPVGTCFDSLHATWDDGASKQNLQFGDGTNTNTGAQLS
                     FMLATGGKVNACFECWNFGQSNNLTSFAILNDQKKSAMGWFNTNSGAGSSEQFGDTNT
                     LNFAGASHCNGCNDDLIQIGFENNMSSSVALSYAAPSIGHQFGNQNQATTNTAGGTLT
                     DFVESGTANNFAETAGTNPGIAHAFAYGLQNRVKDDLGPSEIFFEGLYGFDNEADAID
                     PQDVSIFGASNTVHSLVLCSVGSCPPAQDSVVYGDQNTVTNAGGVLLAAVHSSVTNCT
                     ACGAFGYNEANSTNNSLFIGMGNHEIEITSNLVTMPSLASASVSPVCASTAGGLEIAG
                     CPSPGGVGTIYTAAYGDFSAAASTTNFGIISGLVTGSLAGPGNLLTQAQLLSVACTAQ
                     NPMVVTDTAQPATGTYVFTLFDATTSSATAVTITIPVSSAANTFTGAGSVTLTAGHRY
                     IWRAVNNATSTGAQLGSISFQCK"
     gene            complement(16689..17063)
                     /locus_tag="VN788_00105"
     CDS             complement(16689..17063)
                     /locus_tag="VN788_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18962.1"
                     /translation="MLVDSGVKATTALGSTITLSAVVVFVINFFKSRSWFPWLTAESK
                     VANRFVAVALSGASALGIHFLYDNGTLTITGLSLAGVGLAGWMWLKSFALQEWIYQSS
                     SMSNKAAAAAQQGTPPAEKNGK"
     gene            complement(17050..17295)
                     /locus_tag="VN788_00110"
     CDS             complement(17050..17295)
                     /locus_tag="VN788_00110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18963.1"
                     /translation="MAVNGFRVGQRIWVEVRCGTVQFDFLGTVLYSTEFADHQYHIQF
                     QGGMALRFTREDLKARRVVVRQQIEESSSQGGANAGG"
     gene            complement(17336..17545)
                     /locus_tag="VN788_00115"
     CDS             complement(17336..17545)
                     /locus_tag="VN788_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18964.1"
                     /translation="MRMDVGYAALLAVGSALFGAGVSWGAMRELVKRLRLDLNGVGKK
                     YGRLRDALLLAVPPEKKDELIRFLG"
     gene            complement(17554..18021)
                     /locus_tag="VN788_00120"
     CDS             complement(17554..18021)
                     /locus_tag="VN788_00120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18965.1"
                     /translation="MRTFCRRLQTASDAERDARRWLAGLLLFYALCLAARLVQAQENS
                     RRYHLYDIVQLAAENPANWKHPLTHAEIAGWVDYEKKEGDGDEHIRVCDAKGIAKMDA
                     KHCVVVECIPELPCKIPAKGAHVRVRGIARYDAENPGHHWWEIHPVEQLAALP"
     gene            complement(18018..18797)
                     /locus_tag="VN788_00125"
     CDS             complement(18018..18797)
                     /locus_tag="VN788_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18966.1"
                     /translation="MRTKIDLLRIFEADRGGASYSADLVMRMSRQDREKRILTILERS
                     QMAPSRSLDVFAERILRYSVSLMPFPRAPKESFFGVENYGGSGASTSAPAAAASASAS
                     AAATSSVGNQLPWLFPSSDFLSFQKYGTTPLPVPGIGVNTPIITFKVPNGRNGKITAL
                     GIDFVANGGAAFVQGELPAQLSFNLTADGVPFQDFGNFTYMPGTASVPLAMAGLMLRE
                     GQTIALTMKNLAIANTTQFVEALIQGYYYSKNLQPKTMGYQ"
     gene            complement(18801..19277)
                     /locus_tag="VN788_00130"
     CDS             complement(18801..19277)
                     /locus_tag="VN788_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18967.1"
                     /translation="MPSPWTTANLKPDYLPGVRLPVRSALVPLIGKMPFWVDLFNEQL
                     AAMDSDENRFTMTEDYWWAGTLASIVGVGGVAVVHPAFAFQLFQLDNGGDEASTSQQK
                     PVNQENYFGTAQKPFYLSKPVLFPAGTQLLCRVQNLQSGSAANLFNIQIVMMGYMA"
     gene            complement(19326..19733)
                     /locus_tag="VN788_00135"
     CDS             complement(19326..19733)
                     /locus_tag="VN788_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18968.1"
                     /translation="MSPATAAPNVAPNGAAPGMYNDLLYFYPLNPPQLLANAVNIAAV
                     VTIDNDSDFLWDRIIANSTGLFSVTLTDRFTSRPLMPSNINGENLAGTAQLPFWLPVP
                     YKLRRTSTIQASFNDRSGAPNTIQFVLVGRKIS"
     gene            complement(19939..20688)
                     /locus_tag="VN788_00140"
     CDS             complement(19939..20688)
                     /locus_tag="VN788_00140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18969.1"
                     /translation="MPRIGRSHSGSPLAFRRRGLEKCSLHELGRPRARVGNPNTGIPE
                     VVVQPLYDIYFVAPGTALPKLTLFTVQQGQQYNSGGVAAFNKGPGHTNIVQPGMLESS
                     YTFIVRALSVYVQALQGQAHPNLHPEDLNNFLSSFSRFEINHKSYFDGILGWLPAAGG
                     PTVSGFGTLTAPASMWVAGNGTPQSGNMYKIPGGQTILPQENFDFIIDPTGNAGGNPS
                     TLAAAGNPAGVPGAGLSAWARLDGTLTRVAS"
     gene            complement(20803..21459)
                     /locus_tag="VN788_00145"
     CDS             complement(20803..21459)
                     /locus_tag="VN788_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18970.1"
                     /translation="MARRKRHHRRRRRSRNPFRARRRNAIRYMARNPHRRIRRRRNPS
                     FGLPSQFGEKVGGALIAGVFGARMLPQLVLKENNSGIMGYAADAAGGGLVSWAMGKFI
                     GSAWAEGGWIGTAVAVASRIIADKFGASFGSQGLSGDLDFDLGYYLSDKFPYYPGNAM
                     GPFKTYPGQRYLTPGAVPTTATAVQAGTAAAAALQHAAAAAGQASLSGEGDRWGSSWA
                     "
     gene            complement(21683..22300)
                     /locus_tag="VN788_00150"
     CDS             complement(21683..22300)
                     /locus_tag="VN788_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18971.1"
                     /translation="MQTKNLTRTAADELSYQNQLVRTGQQEYIGTIVSIAVGDDADYE
                     RPIGDPLIDRWGRLMQFGNLALPLSQSLDHSCLLVPGPLRRSDGVVDLPDWKDPLWRA
                     SNWSADEAEGRVLRDPKKDIAPLIPQRLADRTSRMLENSNFLVFQPVLYVVVRPELEG
                     EMLSTFWKIICGPRGSDGTQMSFLVDRATGESHFFGGRYEIVAIE"
     gene            complement(22340..23155)
                     /locus_tag="VN788_00155"
     CDS             complement(22340..23155)
                     /locus_tag="VN788_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18972.1"
                     /translation="MYLTSNSVLAQRRVMLGFLGATTKDPPQQPSSSNAAAAMSALGI
                     PSNDAYYNWTLEAIEQGVLPDYEPGVLTGTAVQFSGGTSGCSSEKTSAKAALTGAVGG
                     TATSVGGTILTAGAASSVTPVGWALLAVGGILTGLSFLFNHHAKAVAAERGTLCAYVP
                     AANAALEQLAEWFQAGQITAQTYIQSLQSVQSQFASGTRSVSSTTNWTRGTCNEGCIY
                     NRALRGVVAKLTADLQTSASGSVAAGSAGAVQAGSSSLLLPVAALAAAWLLLS"
     gene            complement(23156..24076)
                     /locus_tag="VN788_00160"
     CDS             complement(23156..24076)
                     /locus_tag="VN788_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18973.1"
                     /translation="MASVAQFRIQHVATRPRGWRVRTKRHGAHELRIAFPPGKRKGSG
                     QLLEILHPKGERNPSCTLAGKNPAELVIFGNPSKAVKKNARRGNNHKPGCSCPICKNM
                     RGAKKLTAKMPKPVRGVFDRNPKRKRRRRNQDESSEAVKLYQAFHGKDPQGIVEKHVS
                     AAVRMEYTALGKLLALGIGRADASGDALVQRWEKQPHINFEGDGITLASSPDGAQLYC
                     IGGNQNLENCLARFGVDESKDLIDVGDCGFVVYEARKAQGGYEPVEYVHKFAEEKASA
                     ELPRLIFDKLRKMIFFAGGEYRVEAPGIIN"
     gene            complement(24092..24316)
                     /locus_tag="VN788_00165"
     CDS             complement(24092..24316)
                     /locus_tag="VN788_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18974.1"
                     /translation="MKIRGHKDDKSEAKGKKKSKDKKERKHHARCGDVGEVGEHGDLG
                     SNAADAEPEEIDDDDEPSEENEEGEEGDGQ"
     gene            24412..24918
                     /locus_tag="VN788_00170"
     CDS             24412..24918
                     /locus_tag="VN788_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18975.1"
                     /translation="MKLHPATSFFLGIGVGAGALFAYFMLIAYPKIKSVMRGQEAVAT
                     AFTQMYESKRSALLTCQVNLQNARVSSVMIAEPQLPSDQVGEFFGSILGAFFGHPQAA
                     QQLATQLTSTGMKPRFFINTANYKIGSYSTEGNAAVFYYYDPAKLQWVGPNVIPPVNT
                     LITENPVK"
     gene            complement(24896..25507)
                     /locus_tag="VN788_00175"
     CDS             complement(24896..25507)
                     /locus_tag="VN788_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18976.1"
                     /translation="MSVLSMVPLIGSAAQLATPDTMSQANLIAAQLRQANSRLIALEQ
                     SLITNMGNQDYQNQLRPGVNTARARYEKILNAYIYTYGAVFGNPPDTTGLEQWAIYIG
                     AGIIALGTLLAAAYEINKYIDVLQEQQSNITLSAQNTAYAEQQCHAAQAAGDTDGANR
                     WCAVAQSSAASTAQKSGFTEWLSENWAWVALGAAGALILPDFL"
     gene            complement(25507..25818)
                     /locus_tag="VN788_00180"
     CDS             complement(25507..25818)
                     /locus_tag="VN788_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18977.1"
                     /translation="MSYLLNVASPSDMANFLRQQNVPAVPDLGYLSGRPNQAIEHGIY
                     NVLPLRPVTHLSSIFDDATLNMKFLIGGVAIVAAGLILLGRKRSGRRRRMRHYRRRIL
                     S"
     gene            complement(25815..26207)
                     /locus_tag="VN788_00185"
     CDS             complement(25815..26207)
                     /locus_tag="VN788_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18978.1"
                     /translation="MYIMPNNPNLSNNPAAGLTPVDFASPPELSPNPAGIPLPQPTMN
                     APLSVVAPPRKLANTYGIADAPPLLPSGAPALPVAYPNTLPNPAPPAPGTPWLDQQPI
                     LGVPNKYLLGGVLAIFLIAQASKKGAHR"
     gene            complement(26207..26695)
                     /locus_tag="VN788_00190"
     CDS             complement(26207..26695)
                     /locus_tag="VN788_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18979.1"
                     /translation="MYLMPATPLRAVRPSRAQLSGDRERRRRSISLSGLACHCRGGHY
                     LDGLADDGVAIQPSVISTDLTPPADTSYVNPLPAVLVPDTSGQIQTVAPTISTTLTPP
                     VSSAPAVVSSTMAPVMRGAAAPGPKLPFLDQQMITGVPNKYLVAGLAGILVLASAANK
                     RR"
     gene            complement(26695..27420)
                     /locus_tag="VN788_00195"
     CDS             complement(26695..27420)
                     /locus_tag="VN788_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18980.1"
                     /translation="MYLMPATSARGVRPRLRARGMSGLGLITRSILDNQAIPEFFPVR
                     PFPIFPFPAPPQPVHILPVVAPVPPATAPSGSSLPTPSSGMCVVESSPATGGVSSIVP
                     CGNAGAGGGQVISPVNPANPQTFAPAPANYSNLLASPVPASYPKTQPYFAADGGVWNW
                     NPTSGVWVETQLPGSAAAALVSQGAAAPSVSVTSAAPVTESSYQSVIDWFNSESLISG
                     VKNFWILGAGFLGYLLITRKRGR"
     gene            complement(27420..28280)
                     /locus_tag="VN788_00200"
     CDS             complement(27420..28280)
                     /locus_tag="VN788_00200"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HXU18981.1"
                     /translation="MPMPSTVSRIPLGDDGTAATIARMRALIDQGVKHPVVHEAAAFI
                     LRSSKIPQFDAAGEARAIYLWVLRSVRFTRDIVGKETLHSADEILRLGIGDCDDFTIL
                     LCALLGTVGHRARIITISSVPRDVDGEQPFTHVYPEVFLSGRWVPVDAARRNPAFGRG
                     PKRFTRKRVWSLYSDEYVDVQGLNFYASGYGQAQPQRGRYRGGMGDINWGQIIPELTT
                     GTSNIITASRANPINLMPTTSQSGLPSGVPGYGAFSSPYASASFGGISTSTLLLVGIG
                     LIAVMAMGRR"
     gene            28566..29915
                     /locus_tag="VN788_00205"
     CDS             28566..29915
                     /locus_tag="VN788_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18982.1"
                     /translation="MPRAASVSEVPSVVINQNSPVTEQVEEKAKKPRIDFWPYIASLS
                     PTDWRDTIVYLYRDKPVVGSKNKEKYLDVLSAAFTIDDIKQRFGGEEFHAMLVKNGKI
                     LHTEHFSIEAAPKFDSAREIPGADASNAAIVDRLLDKLEQREENPASSLLTTAMTKSQ
                     DMMAQAFETALAKVAGASAGGGGADLIKTIQVLKELGLIGRQENTAVETIRMLKDLGV
                     IGGAAQQPQNQFEIFRSMLAVVKELSGELGENSHPGNWKARLVDKAVEAAPSLIGQVG
                     SIMDKQAAIEREKTNRANMMINARAAMPGAPAVAAPTSTDHIPVPPPAPGAPAPAPMH
                     TVKFDRNSAPANVEAPAPQTAELTGQPSQEWVSRRVVAAIEAGQSGEAVVDFLDCINP
                     MICQYFASLDAEGIRAKFASDAILSQAVALPRFSEWLAEVVEYLHEEGEESPGRRPN"
     gene            complement(29970..30272)
                     /locus_tag="VN788_00210"
     CDS             complement(29970..30272)
                     /locus_tag="VN788_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18983.1"
                     /translation="MDEEKKILATDTEPDSSSNDAHPGEQPVEQLHGICPYCLGVQQG
                     LVFDFFENVMNVAGRVCTVAYAMCSCAKCGKIISTMIVGMQEAKVQPSSFRNLRRM"
BASE COUNT         6125 a         9531 c         8683 g         6054 t
ORIGIN      
        1 attttttttt cttcgtccac ttttcctccc tcaaaacatc cgatgctgcc cgttctcgtc
       61 gactaatttt tcaggcggct tatcggctgg tttttcctcc ggctgtgcct tgggacacgt
      121 ctccgtggtg tgctcgatga attgcgccgg ccactcgacc gtaaccgcct gcacccacat
      181 cttctccgcg cctctttgat acgcgaacac cggagcgcaa acgcaccagg caacctctcc
      241 cgtcgcaacc caaccctcgg cgacgatctc atctcccgtg gccggccacc gcaaaattct
      301 cttcctttcg gcagcgccac attgagtcca aaaaaaactg cattaccacc tgcaaaggcg
      361 aactgttttt ctcggtcatt ggatgcttcg cgaaccagcg cttgattccc tgcgcgagga
      421 tgcgcgcgct ccgctcgtcc aggaaatcgc gcgccggcgc cgagaagaga ttttcggaca
      481 tgtcgagcac ttccgcggcg gtgtagccgt caaaatccat aaaaatttct tcgatcccgc
      541 cgcgatcgag cagcccgtcg cgcagcaact caaccataat caccagcacg tgcccgcaac
      601 tgatcgaata tcccatgctt cgcatttcgc cttcccttcg ccaaaacact taaccgatta
      661 agttccccgc cccgccggca acccgctcgc gtctactgta aagacagtag aatactcgcg
      721 gcacgtccca ggaaaatcac cagaaaaaac aaagtgtttt gaagtgcaat aagcacaccg
      781 ccgctccctg cgtcccccac gctcggattc cttccaattg tcggggacgc ggatttcacg
      841 gtgatgctcc atcgccaaaa accaatcccg cagacgcctc gaattcactc attcgctcgc
      901 gagcggaggc cttcaccgta tccagatccc gccgcccttc gccgcacagg cgcgacgtca
      961 aagaccatcc gcgatcttct ttggagtacg gctccccgag catccctttg cattccgggc
     1021 aaagcccggt gtccgatccc gtgtcctcgc ccggctcgcg ctcctcgtgg aaattgtaga
     1081 agcaggccac cgcatgcacg cggcgcacgc gatgaaaagc cttttcgagt gacgcgagcc
     1141 gctccgcagg agcgtcaaaa aatttgctgg gatatttcag gcagtgcgcc agcgcctgct
     1201 cgaacgactt ggcgatttta atggaaacga tgaacgaccc atccgcgcgc gtctcccgcc
     1261 ataaacgaga aagctttttc tgcttcagcc acgggccaca gtaaatccca tgcgcgtgca
     1321 ggttctcgtt catacgcccg aattcacagc accacagcgc gccgtattca ccgcgggaga
     1381 tcccgaattc gctctcgacg gcgcggaaaa accggcgaat gtcctcattg aaccggcgca
     1441 cttcgtcttt ggtgggcatg cgccccagct tctttgtggt gatgtccagc ttggccagca
     1501 cacaggtgcg caccgttccc gtcaaactcg cctgccagat acgctcccgc ttgcacttgc
     1561 cgccactctc gacttttcgc caaccgatgc ttttctctcc cgctctcaat cgctcgacgc
     1621 agccgcgcaa atccacgtgc cgcgcgaaca gttcgcgaaa acaaaccggc ccgcacgctg
     1681 gacagtagcg attctggcac tggaactttc gaaagtagcg gtgctcgtca tgctcgacgc
     1741 aatcgatccg ccgcgccact gcaccgcaca gcgcctgacg attccctttg ccgatcatgc
     1801 cggcatgaaa caatagcttg gcctgcacgt tctgcgattc tgggatagcg cgcaccagcc
     1861 aggaaggcag ctcgccagcg tcatcgaaaa accgcggaat ggcgcggtcc ggattcccgg
     1921 acggcttggg attctcgcga tcgtgccggc gagtgatctg ctcatcgtgc tcgcgctcct
     1981 cgcagacgta cggctcgttt tcttcggcgc gctcacgcgc tttgcgcaaa ttctttggac
     2041 gccaatgacg catgcgcgcc gcgtcaaacg caggatcgtg ccagatggac tcaggttgcg
     2101 ctgccatcgc ccaccgctcg cggctgatgc agctcgatgc aacgcatcac gtcaccgcgc
     2161 gaaattagga atttactacc acgcaccact tccggaaaca caagccctaa atcacgctcg
     2221 agccactcac gcagcgtgtg ccgctcgacg ccgtattcat cctcggctcc gcgcagtgtc
     2281 acgtaattag gatttttgct gtgcatattg accgatcaga ttgtaaaagt cctgaatcca
     2341 ctcaagtgtg agtttcacca ctacactact ctcggccgtg tcacccttac ggatcgacat
     2401 gaaccactgc tctgcaggac gtgaagaatc cggacgcaaa tctggaagtt gggaataatt
     2461 acagccccga agatttgcga ttgttcctac caggcaggcg cattcacctt gataaacaga
     2521 gccatcaaca cgcccttctt ttagcgccaa tgccaatccg tgtatttcat gtggaacgtg
     2581 cagcaatacg ttccataagt catttttaaa aaattcaaga ctcgcgccgt cgagacgcgc
     2641 gccgacaaga ctcgcgccgt cgagacgcgc gccgacaaga ctcgcgccgt cgagacgcgc
     2701 gcagtcaaga cgcgcgccga caagactcgc gccgtcgaga cgcgcgcagt caagacgcgc
     2761 gccgtcgaga ctcgcgccgt caagactcgc gccgtcgaga ctcgcgccga caagactcgc
     2821 gccgtcaaga ctcgcgccgt cgagactcgc gcagtcgaga ctcgcgccgt cgagacgcgc
     2881 gccgtcgaga cgcgcgccgt cgagactcgc gccgtcgaga cgcgcgccgt caagactcgc
     2941 gccgtcgaga cgcgcgccgt cgagacgcgc gccgtcgaga cgcgcgccgt caagactcgc
     3001 gccgtcgaga cgcgcgccgt caagactcgc gccgacaaga cgcgcgcagt ctagattcac
     3061 atcttgagca accagttttt caatgccctc ttttagtgac tctgcttcca cttcggcgat
     3121 cactactcgt gtgtcccagc gatggatttt gaatttttct ttcacagtta tcctgcctta
     3181 caagtaaagc ggggctgtgc cccgcacccc cgcaaggggc tccgccccct gcacccccgc
     3241 cgttttaacc ccgacccgca gcaactcacg cgccgttttg ttcctctggg aacgcatccc
     3301 tttcgtcgaa atcaaacacc cttcggccag cagcgtccag gctcgcgaaa tatgattttt
     3361 cgtctacatt tctgcgcatc gcgtgggaga tcgcgaactg caccatcgca cccgtcgcac
     3421 caaattcacg caatttatcg tgattcagtg cgtagtcttc catctcgcgt gccgcctgat
     3481 tatttggaac tgtgacaccg acgatggacc gaaaagattt aatcctcgga tatgacccag
     3541 gatgctctcg taaccagcgc aaggcgcgaa acatgatgat ttgacagccc cactcgtagc
     3601 catagcgttt taattctggg tccatcccgg aaatatcgac tgtcttcatt cttgtactcc
     3661 aggttcggcg gtggccggcg tctgcggtat cggtcgcggg acgctcggca gcgttgcccg
     3721 cactgtgcgc gttcgccggc catcaccttt gcccccagaa gagggtaact cgccaccgcc
     3781 cttctaggcc cttcgccctt tggtgattcc acgcggcatg ttgcgtgtcc cactcccgcg
     3841 tgaggtactt cttagcgagt cctcgcgact aggcccgcgc cgcggccgcg tcgggacggt
     3901 tcacttccac ttcgaaattc acctggtcgt tgcccttgtc cgtttcggtg gttccggtgg
     3961 agcgcaggtg gatctgatcg ccgatgagca ggccgctgaa tccgccggga acgccggcgg
     4021 catccacggc catgcgcagg cccgcggaag cgcccacgga aatgtttttt tcggatttac
     4081 cttcgagcat caccggagag accgtgcgca gcacaatggc cagcccgttc tccgttttga
     4141 atgtgcggat cactgtgccg gaaagttccc ggcccttttc ccagaagtcg gcgcgcagaa
     4201 acggcgcgct ggcgtcctcg gtcctgcctc ggaattgcgt catgctgttt ttctcctttg
     4261 gggttttacg tttggtgcca ctgctaacga aatttccacg gccacgggat ccactgatgc
     4321 gcagccagcc atgcaaaaat gcatatcact gccgtggctg tcgcgacgaa agcggcccac
     4381 tcgaaatgcc gctgcaaaaa cttctcgcgc gctacggcca ccgccacttt tccggagccg
     4441 tcgcaccgct cgcacggatc ggcccaggaa gtcgcgcctt cgcccccggc actccgggca
     4501 cggcgccgcg gtgaattgca ggccgctcac cgtgaggacc tccacttggc caccgcgttt
     4561 tgcagctccg agaacctatt ggcgatcgac acaggaatca gtcctcggta cttctcgaaa
     4621 aatggagcac agaaatccac aaaatttgcg gcagcccgtt ccactggtga cgccggtggg
     4681 gttttgggag agctcatttg cgtttcttgc ccttccgtgc agacgtctgc actttggcct
     4741 tgcttgccga cttttttgat ttgatcgcgg ccagggcgcc gctttccatc ttggctagag
     4801 ggacatgcgc ggccttggct acgcgctgta atctctcttt atcctcgccg tggtaatcgt
     4861 tgttccagcc gccaaaagcc ggcgtcgcgg ccgcggcggc gagtagggga acaattcgcc
     4921 gcgcgaagtt ccctgctttt tgaatctccg ctacgcgctt ttcgattgcc tcgcggtaat
     4981 caaatccgac aaccttgccc ttcgccggga cgatgcccaa tccattggcg aggagcttcg
     5041 ctgcgtcgtg gtgtagccgg cccagcagag cctcggcgat cacgccggcg caaacgttga
     5101 acgagagttt cgcttcggcg atttttccca gcgccagcgt gcgaactttc gctatggcgc
     5161 gctgcttgcg atcggccgct ttttgcttcg ggctcgtgcc ggaaaactgc gagacgtctt
     5221 ccgcgccgta cggatggtga actttgcact tcgcgtccgc gcagatgcgc ttctcgatgc
     5281 ccactttggc gtcaccgtgg tacccgccgg agagcaccac tacggcgtct ttggacgacg
     5341 ggcatttctt gtccgcgaac gtgtaaccgc ggttgccctg gtaaagcacg cccgggacgc
     5401 ccttctctcc gtactcgcga tagcccgacg aaatcgacac cagtccgttc tttgtgttct
     5461 gctcgacttg cgcttccagt ttttttctgt agcaggcggg atcggtgcaa atgtccttgg
     5521 ccttcacgtc ggggaagagt tgcggattcg cgccagtgcg cttgggacac ttcacacagc
     5581 tgccggcggc gggcagaagg gcttcatcgg ccaggtccca cggcgcgttg cgcagttcca
     5641 ggtgaaagtc ctcgtgaatt ctccgcgcca gcgcgcgcac gccgccgatc ccgtcgtcgc
     5701 cattgcgccg gatgtccgcg aggagtttct cctgctcgcg cggctgcaag cgcgcaatca
     5761 gaatggcgtg ccctgcggtg atctcgtcgg agaaaaacat cttctgcgcg gcgggaatga
     5821 gcgcggccag cttcagccgc tggtagacgt agctctcgct cttgctcact ttcgcggcga
     5881 tcgacggcac gtcgtagcgc ccatccctca aaagcgcctg atagcccaaa gcctcgtcca
     5941 gcggatgcac atcttcacgc tgcaaattct cgatcacctg gaattcgagc gcctgctcgt
     6001 ccgtgagcgc cagcacgcga cagggcaccg ctgcgatgcc cgcggatttg gccgcgcgat
     6061 agcgccgcgc gccggcaatg atttcgtagg tgagctcgcc gccatccgtc cgacgcacca
     6121 gtagcggcgt caatacgccg tgctgcttca cgcttgcggc cagctcttcc acgccggcga
     6181 agtgccgccg cggattggtt ttcgattcgc gcagaaacct cagttcgatg gtggtgaacg
     6241 agccctcgag ttgcaacggt ggagcgggaa cggcgccccg cattacggca gtgctcattt
     6301 tcaaatatcc tttcggctcg aacccgagca ggaccaagta ttcgtcatac aagcatctcg
     6361 gctctgtcgt cattttccaa agatggtcga gacgcggtcg attttgtgca ggaacacttc
     6421 aatgtgaccg ttaagctctg caagatgctg attcatccgc gcgacctgca gaacaatctc
     6481 gcccaaaaag aaagcgtctt cgcctttagc tttgtcccgc gcttcctgca actgctgttc
     6541 gtctcgctcg tccatttcag cctcgcaaaa ttatttaccg cgctggcaac ttaaatgcta
     6601 tgtatccacc gtccccgcaa tccaatgcaa aggaactact gccatgaata tgccgttcgc
     6661 ttacatcatg tcgtgttcgg agccgtcgct cggggagttt tctctcgccg ttctcaaccg
     6721 cctggcggaa gccaaggccg cgcggcgcga tctcgaggaa cagatcgacg agctcgaagg
     6781 caccgcgcag ctgattgaat ggctgcgcgc acgccccgga gcgcgcgctc gtttgctgaa
     6841 cctggaattg ccgcagggag tgttttcgtt catgcgcgaa gaaatcaaac ccgccgcgct
     6901 gccgcccaga cgcgaagaga gagccatgcg cctggccggt taggaaaaat tcaaaaaggg
     6961 aagtgctgag agagggaaga aaaagcgctc ggttgaagtg tgtctttttt ctggcgagcc
     7021 gcgcgagcac cgaaaccctc cggcgtttcg cgcttggtta cgttaactcc gcgtcataaa
     7081 cattaggcgc agtcattgca cgaaacgggg aggaatatgc tcctgtaccg atgggcaggt
     7141 caagtgtttt ctgtggtttt tcataacaat ttttcacgtc tatatgtagt ggtgtagcga
     7201 tggcgaacac actacctatt gtggtcacaa cggacgctcg agtgcagttc tggtacgttt
     7261 cgcgttttat tcgccaaagg gaaaactagt tggggagcac gaggcggaag aaatgcaggc
     7321 ggccgatgtc taccgttttc gcgaactggc cggacttggc ccatagcggc gggaagtcgt
     7381 cgagcgagcg atcgtagtac gacacggcgc cgccggtggg atcggttccc tcgccgctat
     7441 aagcttcctc ggccgcagag atggcgtcat tccacaccgc cgcgccgggc acgggataaa
     7501 tcagcaaatt aggatcggcc tcgaggccgt tgatggagct gtactgccat tttttggtga
     7561 tcacttcgat ccaggagcgg ccccaccacg agggacgact gacgcggttg cggatggacc
     7621 aggccaccgc gcgcatggaa tccagtccgt cgccgcgcgc ttccctgccg gtaaccagca
     7681 ccagcatgga gaactcaaca gcctgcgatg aactcagttg caagggcatt ggctatccac
     7741 ttttgcgcaa ttctttgtac aaaaaccgaa ggggcccgcc catgcgtttt ctctcgcaag
     7801 agcgggcccg attgtttcgt gtttgttaac gttccactga tcaatcataa tttaggcaaa
     7861 caaatagcga aaagtcaagg gagtaaatcc cttgccacca ggtacaccgc gagcacgccg
     7921 ccgaggatca tagccagtgc agccatgtcg ggcgaagcag gaggcgcaac cgccgcgggc
     7981 gccgccggca atggcgcaga agtgcccacc acaaaagaat ccgtggagcc cggcacgccc
     8041 tgcacgccct gcacgccctg gcccaggaac gtgccaatca tcgccgcggt gctcacgccc
     8101 aggtagctcg ccacgttgga cgcccaactg gaatctcccc cggcccagat ctcgcccatc
     8161 tgcgcgagtg tcatttccgg agagtaatag gaactttgtc ctgcggcgat ggtcgcgagc
     8221 tgctgctcga gcgcgttcca gccggcttgc gcggagggga aaacagaata gccgccggcg
     8281 tcggtagaaa cgcccggcca atttttcagg tctccgggat tgttgttgcg cgtaggacgc
     8341 gccgcaggcg ggccgtaacc ctcggcataa gcaatcgcgg ccgcaaatgt ggagcgcgcc
     8401 ggcattcact cctcgtcgtc atcatccccg atttcgtcgt cctcgtcctc ttcgatctct
     8461 tctcgttcat cctcgtcaag gtcgtcgtca tcgtcctcga aatcttcggg gctgggcagc
     8521 tcgtccactt ccacgatgtc gtcgatcaat tgaccttctc ccgcgccgct gttggcgccc
     8581 tgcatgtact tgcaatgcga caggtgctcg tcgtagtagg tgcgcgcttc tttttccgtc
     8641 ttaccgtaga acacgtgcgc catcttgatg gtgccgtcgc ccggatccac gatgtaactc
     8701 cacatctcaa cggccacggc gcgcctccca gtattcgcga actatttcct cttgcctgcg
     8761 gtgacgcaac tctttttcgc gcagagtgat ttcttcttca cggtgacggc gcaattcctg
     8821 aaaacccgcc ttcattcccg caagcagcgc gcgcacaagc tggctggcgc aataaatcac
     8881 cagggccgcg agcgcggccc aggcgacgat gccgaaaatg tgcagcgtat gcatgcgtgc
     8941 cactgtaacg ccgctgtcaa gccccttcgc gcgcagctac gtcgcgctgg gcggattcgg
     9001 ctgcctcgcc ggcgggccgg gcagcggcgg cttgcccaaa cgcgcgagct cgccgttgat
     9061 ggcctcgaga tccgcttcgc tcagcgattc cacgtcggcg agcgcctgca cgtcggtttg
     9121 gaccagcacg gaatacgtca ccgtgttctc gccggtcgag agctgctcga ttttctttac
     9181 cgtccctttg atcagtggaa cgatttcgcc ggccacttcc acgcccaagg tcacaatggt
     9241 gttggctttc gacaaattcc ccagcacgct atcgagtaca ttgctcatct gatgccccct
     9301 tatttttcgc cttttcgtcg cccggggcgg tcgaaagggc actttaatct tgcgttagga
     9361 cgcatttttg gtagttctcg aagcatggaa caaaaacctc gtatctccgc atcccacaaa
     9421 tccgcaaaac gcattcgcga gcacatcatc gtgagcgtca gctcacgcac gcgtcgcaaa
     9481 tgccagtacc cgccgtgcgg caaatcctac catcccaaac gctactggca gcgcttctgc
     9541 tcggacaaat gcaaggccga cgactggcgc tcgcatcatc ccatgaccta cgagcccaag
     9601 cccttcaaag actaaccgaa gaggctcacg atcgcgcttg catctgcggc cagttgcgcc
     9661 accagcgcgc tcacttcctt gtggcggttc gggtctttga ttcccgcggc gccgctcgaa
     9721 gtcaatcccg taagcgcctg ctgcacttgg gaaatgtagg cctgtacgcg ctgctgtgtg
     9781 ggttgagtcc aatcgtccgg aacgctttcg agaaatcggg aagcctggat ttgcgcagtg
     9841 tcgatgcgcg cgatgccgac aagcgtctgc gtgtaagcgt cgccgtcggg aaacgcgccg
     9901 ctgcgattga gcgacatcac cgcctggcgc agagagcttg tgaccgtagc gattttctgc
     9961 agattggcca ccgccgcgtc ccactgctgc acttgctgga cgctcacgcc ggcgggcgcg
    10021 ccggtgacgt ttttcacgcg cgcggcgcaa cccatgtaac ccagagacgc aaagagcaaa
    10081 ataaaaccaa acacaatttt cgttttcaac tgccgacgaa atgaggcgat gctcatggtc
    10141 gaattctcct tgatttttta cttctcacct gaaaccgtga caatcaattt gctgggggcg
    10201 atgggcaccg gcacaacgcc tccctggaac cacaatttat ttgcggcaac cactcccgta
    10261 gcgccagtga gtccgttgcc gaagggcagc actgccggag ccgccggcgt gggctcccct
    10321 ccacaagtga gagacggatt caccacgcac ggcacagcgc cggcggaagg ctcgatgccg
    10381 ttatagtcgt tatcgctggg cgtggtccat ccccaattat tgacttccga agcgttgaaa
    10441 tccgccatgc cccagtggtc caagccgacg cattgaaaat cgttgttgaa tcccagtgtg
    10501 ttcaaacaca aatttttcag gttgtattcc gcggtgccgc gcgcggcctg tgtagcgtaa
    10561 gcgccgggac tcgattggca cgaagtgcac gaggacaccg ccgaatccgc ggccgcctgc
    10621 aagaaaatct cgtcgaatat cggatgatcg ccgaagtact gctgctcgaa ggacagccgc
    10681 tgcatctcat cagtgggaaa ggtgttgtaa atggagctga acaacacgtc gagataaacg
    10741 cccgcggctt gcaggatctg gctgtgcgcc ggcgtgttcc agtcgcccag cgtattcggt
    10801 ccgaagtaaa gcaggttagg aaatgtggtt ttgagcgcgg tacgcaccgc cgagaaatac
    10861 tgcgcggcat cttgatactg ccagttgctc aaatcttgcc cggccgtcgc gttcatgaac
    10921 gtggtgggat tgtttccggg attccccgcg cggcaggaat agcctggcgt ccctggcgtc
    10981 gctcccccac cgttcaccag gcaaattgaa ttcaccccgc caccgagcca agcatggcgc
    11041 ccgtcttcat ccatcaagcc agtgccccac atccatccgc cggcgatagc gttaatggtg
    11101 atggccgctc ccgttgccgg cgcggtggaa aacgtgagct gcacggcgcc tgtgccatag
    11161 tccaggaacg attgcgggcc ggggacggcc gctccggaaa tgagcccggt ggaggacact
    11221 ataaaattcg tggagagcga cacgttggtg gcttgcaaag taggcgccgg ctgcgatgca
    11281 gtgctggcca cgcaaccaaa tgagggagtg ctgcctgaag ataagcggca agccatgtac
    11341 acgtcatagc cggtgacacc cgccggagga ctcacgggcg cgaggacgct gtcgcctttc
    11401 cctgcggcgc acgtttctcc ggtaagccgg ctggcgatgt acgtccctga accgtgccac
    11461 aggacgatag cccagaagct cgccgcggga gcggaagggt tcgcgcccac tgccccgcag
    11521 ggattatcgg aaagaaaagc ctgctgcccg gaagcgagcg tgctggtggc atcgcccaga
    11581 atgacccctt ggcaagttcc cgccgtggcg caattgtaag gcgtcgaggg attggaaata
    11641 tgcgcgcagt tgttctccca ccaggcgcaa tccccgccaa tctcggtgct gttttcaaac
    11701 accgccacag agagcgggga aatgttccct gccgcagtgg gcagcgtgca ggtgaagagg
    11761 atctgcgagc cattgcccgt gccgcagttg aacgcgctga tggttgtgcc ggaagagccg
    11821 aaagtcgtat aagtggctcc gccccaggct gtgttgagcg cggaaatcga tccaccgtac
    11881 tccgcgttca agtaatcggg cagcgagcat cgcggcatgg tgatactggt gagcgaccag
    11941 cactgcgaaa gggatgtggg cggcgtggcc gacaaatgct tgctgtacac attggtgtcc
    12001 gcgtagagaa tcacttttcc ctggtaggtg cgattgccgc cattgttgag cgtctcgacg
    12061 ggagaggtct gcatcaccac catggcgaga ttggctttgg agtgcccggc ggggaaagtg
    12121 tcaaatgcat ccgatccgcc catgccgcac tggaaatcgg tgtcgtccac agtcaatccc
    12181 aaattgtgcg cgttcaaaaa attgtgctgc ggagcgagat tgttggtggc aaagacctgg
    12241 tactgcggat cgaacacgtc cgcgcatccc gccccggtga aggcggtaaa ataatcgttc
    12301 actccaatga tgtaattctt gacaggatgt gacgcggcgc cgttcaggtt cacggtggcg
    12361 tacacgctgg tgttggtgtt ggtgaaccct ggcgtaacca ggcagccggt gcatagcgtc
    12421 gagctccacg gcaggtacat cgcctgggag tcctcggccg ccacgttata gccatacgac
    12481 cgcaggtatt ttgattgcgt gtctttcgcc gcggtctgcg tggaattccc gtacttggca
    12541 agaactccag aggcccaaat cacgctggcg ttgatgccgg gcacctgcat attggccagc
    12601 gcgtttgcaa aaaaatggtt tccttccggc gtgcagaacc accagcgatt tccggctttc
    12661 tcggtgtaga aaaatccctt cgagcctccc gagcaaggcg actgctgcca gccgccatca
    12721 gcgtcgaatg tctgcgcttg cgcccccgcc gcaaatacag agagaagcgc gcccagcagc
    12781 gcgccgatcc acagccgccg cgctagttgc attggaagcc cactccaata atctgcgcgc
    12841 tggtggccgc ggcgttgttc accatctgcc aggcgtaaag gtctcccgcg gttatcgtcg
    12901 cgcttcctgc gctattgaat ttgccggacg cagctcctgc gggaatggtg atagagaaac
    12961 cagtggctac cgtggtggtc acatcgaaaa gcgtcaacac cagcgagttg agcgcggatt
    13021 gcgcgctgtt ggtgacggct tcgccggtcc ctgccgtgca ggccaccgtt atcgggcccg
    13081 cccgagtggt gctcgaagca tttccggagg acgaaccgga tagtggccca aaaagcgtgc
    13141 tggaagccgc tactgtctga ctttgcgcgt tccagctata aagcgtgcct agattcgtcc
    13201 tcttcgagat cttcaccagc caaatcgtac cggcggcatt cgcgttgagc gtggccgtct
    13261 gtccggcgga gagagtgaag ctggtcactc cctgcgtgtc tgtcaaaccg tccagtacat
    13321 tcagcgtggc cgcgccgccg cgattcacgg tgagcgtgcc ggcgccgtca tcgtagagcg
    13381 acactacaaa attccctgcg catcctgcgg ccgtcggatc cggcaaattg atgacacttg
    13441 cgcccgtttg gaaatcgatg gtggtgctgc gatcctgcac cgcggtgccg ctatcgcaaa
    13501 gcacggcgta aggcgtggac gccactgcgg cgcccgcatt tccccacgcc aaaccgggag
    13561 acgcgaaaac gggaagcgtg gcgttctgcg ccacaatggt ctgcccggta agtccgccgg
    13621 gaacgcgcgt ggccactccg gccgcggcgc cggcgatcat gtctccggca gtggtcatag
    13681 gattagcgaa tcccccgccg gggcaagcgg cgtccgtgta taaagtgccg ttgcccacca
    13741 ggcagtgatt cagcggggcg gcattgccga tgctgaatcc ctgggcggaa ttgaccggcc
    13801 cgctggcgct caaaaacaca ggagatgtcg gattgatgga actgtacgaa acacccagcg
    13861 cgccggtggt gcccacattc aaattgcgaa aattaaaaac gaaattcgcg gtggctgaag
    13921 ggttgtttcc ggtgccgaac accatctgca tggaaaaatc ttccggctcg gacgccgcgc
    13981 cattgaaaaa attgccaagg acgccgatgg ctgtgctact ggaattcgct cccgcggtgg
    14041 ccgggatggt gttttgcgaa acgatgtcat cgtccacggt gagcacttgc tggaactcat
    14101 tgagcgcggt ccaagtgttg ttcagcccca acaggctggg caatccgacg atgcttgttc
    14161 cattgaaacc aagcgattgg ccggtcgagg gcgccgtcgc ggagagcggg atgcgcgtcg
    14221 cgccgaaatg aaatccgatg acgttttgcg agattccggt gccgctcaaa tcgcctccgg
    14281 cggtgaaccc tcctccaccc cctccggtga tatgcgcgag cgcgggcgcg gcggctgaaa
    14341 gcgtggcgct caaatcctgc gaaagcccgg caatcgttgc ggtgaaggaa aagcattgcg
    14401 ggccggttcc gagcggaggg accacgcccg gagaaatgca cacggtgaag gtccactgcg
    14461 tcagcgcggg agtgatgctc gcgttcggat acaaattcag agtgaagctc ccggtggaat
    14521 cgagcgatac cgggcccacc gtcccttgaa tcggcacgca gttcgagcct gtgaacacgc
    14581 agggtgattg cactccagga ggagacagcg tcgctttcag cgtggcgtct ccgtagggca
    14641 cgccattggg atccaccact gtggccgtca cggtggtttg cgattgcgcg gccgcgatgc
    14701 gcgcaacgcc gaacaaaata atcagcaaac aaattagacg tttcatggtc atttgcactg
    14761 aaaagaaatc gagcccagct gcgcaccggt ggaggttgcg ttgttcacgg cgcgccagat
    14821 gtaacgatgc cccgctgtaa gtgtcacgct tcccgctccg gtgaaagtgt tcgcggcaga
    14881 actgacagga atcgtgatgg tgacggccgt cgctgaggaa gtagtggcgt caaaaagcgt
    14941 gaagacgtac gtgccagttg ccggctgcgc ggtgtcggta acaaccatcg gattttgcgc
    15001 ggtgcaggcg acagagagga gctgcgcctg cgtgagcagg ttgcccgggc ccgcgagtga
    15061 acctgtcact agccctgaaa ttattccgaa attcgtagtg gatgccgccg cgctgaaatc
    15121 cccgtaggcc gcggtgtaaa tcgttcccac tccgccgggc gatgggcagc cggcgatctc
    15181 gagcccgccg gcggtcgaag cgcacaccgg agaaacgctc gcagatgcca ggctgggcat
    15241 cgtcaccaga ttcgaagtga tctcgatctc gtgattgccc atgccgatga acaggctgtt
    15301 gttggtggag ttggcttcgt tgtagccgaa tgcgccgcac gccgtgcaat tcgtcaccga
    15361 gctgtgtacc gcggccagca gcactccgcc ggcattggtc actgtattct gatcgccata
    15421 gaccacggaa tcctgcgccg gcgggcaaga acccaccgag cagagcacca gcgagtgcac
    15481 tgtgttgctg gcgccaaaga tggacacgtc ctgcgggtcg atggcgtccg cttcgttgtc
    15541 aaagccgtag agtccttcaa agaaaatctc ggacgggccg agatcgtcct ttacgcggtt
    15601 ctgcaggccg taggcgaaag cgtgcgcgat gcctggattg gtgccggccg tctccgcaaa
    15661 attgttggcg gtgccggatt ccacgaaatc cgtaagcgtg ccgccggccg tgttggtggt
    15721 cgcctggttc tggttgccga actgatgccc gatgctgggc gcggcgtagc tcagcgccac
    15781 gctgctgctc atattgtttt cgaagccgat ttgaatcaga tcatcgttac agccgttgca
    15841 gtgcgaagct ccggcaaaat tcaacgtgtt ggtgtcgccg aactgctcgc tcgaccctgc
    15901 gccgctgttg gtgttgaacc agcccatcgc cgattttttc tggtcattca aaatggcgaa
    15961 ggaggtgaga ttgttactct gtccgaaatt ccagcactcg aaacaggcgt tcaccttgcc
    16021 gccggtagct aacataaaag acagttgcgc gccggtgttg gtgttggtgc cgtcgccgaa
    16081 ttgcaggttt tgcttcgacg caccatcgtc ccaggtggcg tgcaggctgt cgaaacacgt
    16141 gcccacggga tgctcggaca aaattccagt gtccacgccc gagggcgtgc agcctccgcc
    16201 tcctccgcca atcggaatgc gcgtcagctg cggcggcggc gcggcggtga tttgcgcagt
    16261 gatgttttgc gaagctccgg aaatcgtagt ggtgacggca aagcactgat cgcccgtgcc
    16321 gatgggcggc tcgatcacgc cgggctgaat gcagatctga aacgtccact gcgtggtgca
    16381 cggcccttga ttgcaaaaaa tcaaattgtt gggatagagg ttgatggtga agttgccgaa
    16441 gaaatcgatg ggcacggtgc ccactgtgcc ggggatgggc acacagttcg ggccggtgaa
    16501 cacgcacggc gaagccactc cgggagggaa aagcgtggcc ttcaccgagc cgccggcgta
    16561 gaaaaatccc ttgggatcca ccacgttgcc ggtgaccgtg gtttgcgatt gcgcagccgc
    16621 gcgcggaggc gcccaaaaga gcgccagctc gagcagccac aggaaaacga aaagtctacg
    16681 ccgccaggtc atttgccgtt tttctccgcg ggcggagttc cctgctgcgc ggcggcggcc
    16741 gctttgtttg acatgctcga tgactgatag atccactcct gcagggcaaa gctcttgagc
    16801 cacatccatc cagccaggcc cacgccggcg agcgagagtc cggtgatagt cagcgtgccg
    16861 ttgtcgtaga gaaagtggat gcccagcgcg gatgctcccg agagcgccac agccacaaag
    16921 cgattggcca ctttcgattc cgcagtgagc cacggaaacc agctgcgcga tttgaaaaag
    16981 ttgatgacga aaaccaccac ggctgaaaga gtgatggtcg agcccagcgc cgtcgtcgct
    17041 ttcactccgc tatccaccag catttgcgcc tccctgtgaa ctggattcct cgatctgctg
    17101 ccgcaccacc acccgacgcg ccttcaaatc ctcgcgcgta aaccgcagcg ccatgccgcc
    17161 ctggaactgg atgtgatact gatggtccgc aaattccgtg gagtagagca ccgtgccgag
    17221 gaagtcaaat tgcaccgtgc cgcagcggac ttcgacccaa attctctgcc cgacacgaaa
    17281 cccgttgacc gccataggcc ctcaccgtgg aaaccgttca tctgcttctc tctcgctaac
    17341 ccaggaatcg aatcagctcg tccttcttct ccggagggac cgccaaaagc agcgcgtccc
    17401 gaagacgccc gtactttttg cccacgccgt tcaggtccag gcgcagccgc ttcaccagtt
    17461 cgcgcatcgc gccccaggag acgccggcgc caaacagcgc agagcccacc gccagcagcg
    17521 ccgcataacc cacgtccatg cgcatcgggt cgcctacggc agcgccgcga gctgctctac
    17581 cggatgaatc tcccaccaat gatgaccagg attttcggcg tcgtagcgcg cgatgccgcg
    17641 aacgcgcaca tgcgcgccct tcgcgggaat cttgcagggc agctccggga tacactcgac
    17701 caccacgcaa tgcttggcat ccattttggc gatgcctttc gcgtcgcata cgcgaatatg
    17761 ctcgtcgccg tcgccctctt ttttctcgta gtccacccag ccggcgatct ccgcgtgcgt
    17821 cagcggatgc ttccagttcg caggattctc ggcggcgagc tggacgatgt cgtacaaatg
    17881 gtagcggcgc gaattctcct gcgcctgcac cagccgagcc gccaggcaca gcgcataaaa
    17941 cagcagcagg cccgcgagcc agcgacgcgc gtcgcgctcc gcatctgagg cggtttgcaa
    18001 acgtctgcaa aacgttctca ttgatacccc atcgtcttgg gctgcaaatt cttggaatag
    18061 tagtaaccct gaatcagcgc ttcgacgaat tgcgtggtgt tcgctatagc caaatttttc
    18121 atcgtcaaag cgatggtctg tccctcgcgc agcatcaatc cggccattgc cagaggaacg
    18181 cttgcggttc ccggcatgta ggtgaagttg ccaaaatcct gaaaaggcac tccgtccgca
    18241 gtgagattga aactcaactg cgcgggcagt tcgccctgga cgaacgccgc gcccccattg
    18301 gccacgaagt cgatgcccag cgcggtgatt tttccgttgc gtccgttggg tactttgaaa
    18361 gtgataatgg gagtgttgac accgatccca ggcaccggca acggcgtagt gccgtacttc
    18421 tggaaagaca aaaagtccga agaaggaaag agccagggca gttgattacc tacggatgac
    18481 gtggccgccg cggacgccga ggccgaagcg gccgctgccg gcgccgaggt gctggctcca
    18541 ctgccgccgt aattctccac accgaaaaag ctttcttttg gagcgcgcgg aaacggcatc
    18601 agcgaaacgc tgtagcgcaa tatgcgctcc gcgaaaacgt ccagcgagcg cgatggagcc
    18661 atctggctgc gctccaagat cgtcaggatg cgcttctcgc ggtcctgccg cgacatgcgc
    18721 atcaccaggt ccgcgctgta cgatgctccg ccccggtccg cttcaaaaat ccgcaataaa
    18781 tcgattttcg ttcgcatcgc ttaggccatg taccccatca tcacgatttg aatgttgaac
    18841 aaattggcgg ctgaaccgct ctgcaaattc tgcacgcggc aaagcagctg cgtgcctgcg
    18901 ggaaagagca ccggcttcga caaataaaac ggcttctgcg cggtgccgaa ataattctcc
    18961 tggttcaccg gcttctgctg gctggtggaa gcctcgtcgc cgccgttgtc cagctggaaa
    19021 agctggaagg cgaacgccgg atgcaccacc gccactccgc cgacgcctac aatcgaggcc
    19081 agcgttcccg cccaccaata atcctcggtc atagtgaaac ggttctcgtc cgaatccatt
    19141 gccgcgagct gctcattgaa cagatccacc caaaacggca ttttgccgat gagcggcaca
    19201 agcgcgctgc gcaccggcaa gcgcacgccg ggcaggtaat ccggtttcag attcgcggtg
    19261 gtccacggcg acggcatttt ggtttcaatc ctcccgctta cgaaattcga gccagttcct
    19321 cgcgcttacg aaatcttccg gccgaccagg acaaactgaa tcgtgttcgg cgcgccggag
    19381 cgatcgttga agctcgcctg gatggtcgaa gtgcgccgca gcttgtacgg caccggcagc
    19441 cagaacggca actgcgccgt gccggcgaga ttctcgccgt tgatgttcga aggcatcagc
    19501 ggccgcgacg tgaagcggtc ggtgagcgtg acgctgaaca gccccgtgga attggcgatg
    19561 atgcggtccc acaggaaatc ggaatcgttg tcgatggtca ccaccgcggc aatgttcacg
    19621 gcgttggcca gcaactgcgg cgggttcagc ggataaaaat agagcagatc gttgtacatc
    19681 cccggcgccg cgccgttcgg tgccacgttc ggtgctgctg tcgctggact catgttcgct
    19741 tctctcccgc ccgccgtttc gcggcaagcc tcgcaaaatt ttcaaaagtg aaggaatcct
    19801 gcggcgagcc gatgcacagg caaccgcccg ccgccgattc cgcgccagaa acattcctcc
    19861 ggcttccgca gccgtgaaat atctcccgtt tttccgctgc ggttttctct ccgcggcgga
    19921 aaacttttgt gaatcgaatc agctcgcgac gcgcgtgagc gtgccgtcga gccgcgccca
    19981 ggcggaaagg cccgcgccag gcacgccggc gggattgccc gcggctgcca gcgtggacgg
    20041 attgccgccc gcattccccg tgggatcgat gatgaaatcg aaattctcct ggggaaggat
    20101 ggtctgcccg ccggggattt tgtacatgtt ccccgactgg ggcgtgccgt tgccggctac
    20161 ccacatggag gcaggagccg tgagcgttcc gaatccgctg accgtgggcc cgcccgccgc
    20221 cggcaaccaa cccaagattc cgtcgaaata ggatttgtgg ttgatttcga agcgtgagaa
    20281 cgagctgagg aagttgttca aatcctcagg atgcaggttc ggatgtgcct ggccttgcag
    20341 cgcctgcacg tacacgctga gcgcccgcac gataaacgtg taggacgact cgagcatgcc
    20401 cggctggacg atgttggtgt ggcccggccc tttgttgaat gccgccacgc cgccggagtt
    20461 gtattgctgc ccctgctgca cggtgaagag cgtcagcttg ggcagggccg tccccggcgc
    20521 tacgaaataa atatcgtaga gcggctggac gaccacttcg ggaattcccg tgttgggatt
    20581 gcccacgcga gcgcgcggcc ttcccaactc atgcaacgag catttttcga gcccgcgccg
    20641 gcgaaacgcc agcggagaac ccgagtgtga ccttccgatt ctgggcattg ttgcagccct
    20701 ccctcgattc agaatttcgc ccacgcaaag cggttgaaat tcgatccgca accgcgcgcc
    20761 cggcccttcc cctgccggcg cgcggctact tcgcccagaa atctaggccc agctcgagcc
    20821 ccaacggtct ccctcgcccg agagcgaagc ctgacccgct gccgctgcgg catgctgcaa
    20881 agccgccgct gccgctgtgc cggcttgcac ggccgtagcc gtggtcggca ccgcgcccgg
    20941 agtcagatac cgctggccgg gataggtctt gaagggaccc atcgcgttcc cggggtaata
    21001 cgggaacttg tcgctgaggt aataccccag gtcgaagtcc aggtccccgg agaggccctg
    21061 cgaaccgaag gacgcgccga atttatcggc gatgatgcgg ctcgccacgg ccactgccgt
    21121 gccgatccac ccgccctcgg cccatgctga accgatgaac ttgcccatgg cccagctcac
    21181 cagaccgcct ccggcggcgt ccgctgcgta gcccatgatc cctgaattgt tttccttcag
    21241 gacgagctgc ggcagcatgc gcgcgccgaa cacgccggcg atgagcgcgc cgcccacctt
    21301 ctccccgaac tgcgaaggca gcccgaagga aggattgcga cgacgccgga ttctccggtg
    21361 cggattgcga gccatgtaac gaattgcgtt gcgacgccgc gcgcggaagg gattgcgact
    21421 gcgcctccgg cgacggtgat gccgttttct tcttgccatg tgtcctccgc cggcaagtct
    21481 gcgcgagatc atgcgggaga cgacgctctt caaactcgaa ttgcgtcggc gtctccgcga
    21541 atgacgcttg cggcgcgcgc gccggccgcg cgtgcgggat ggattcacca gccttacggt
    21601 tggcattttt ttgcctcttc ccttctccgc tttcgccctc caccccgcca cgcaaaccag
    21661 gaggaacgcc gatcagcggt tgctactcga tggcgacgat ttcgtatcgc ccgccaaaga
    21721 aatgcgattc gcccgtggcg cggtccacca agaagctcat ctgcgtgccg tcgctccctc
    21781 gcgggccgca aatgattttc cagaaggtcg agagcatctc gccctcgagc tccggacgga
    21841 ccaccacata cagcacgggc tgaaacacga gaaaattgga attttcgagc atgcgcgaag
    21901 tgcggtccgc aagacgctgc ggaatcaacg gcgcaatgtc ttttttcgga tcgcgcagca
    21961 cgcggccctc ggcttcgtcc gcgctccaat tcgaggcccg ccacagcgga tccttccagt
    22021 ccggcaaatc caccaccccg tcgctgcggc gcaatggccc gggaaccagc aggcaggaat
    22081 ggtcaagcga ttgcgatagc ggcagcgcca gattgccgaa ctgcatcagc cgcccccagc
    22141 ggtcaatcag cggatcgcca atcggccgtt cataatccgc gtcgtcaccc acagcaatcg
    22201 aaacaatcgt gccgatgtac tcctgctgcc cggtgcgcac cagttggttc tgatagctca
    22261 gctcgtcggc tgcggtgcgc gtcaagtttt tcgtttgcat ccgtgtcatc ccgattccct
    22321 tccctttgcc tttacgcaac taactcaaaa gcagccaggc ggcggctaat gcggccaccg
    22381 gcaacaacaa actcgacgac cctgcctgaa cggcgccggc ggaacccgcc gcgacgcttc
    22441 ccgacgcaga cgtctgcaaa tcggcggtca gtttggccac cacgccgcgc agcgcgcgat
    22501 tgtaaatgca gccctcgttg caggtgccgc gcgtccaatt ggtggtcgaa cttacggatc
    22561 gcgtcccact cgcgaattgc gattgcaccg attgcagcga ctggatgtac gtctgcgcgg
    22621 tgatctgccc ggcctggaac cattccgcga gctgctcgag cgccgcattc gccgccggca
    22681 cataggcgca cagcgtgccg cgctccgccg ccactgcttt ggcgtgatgg ttgaaaagaa
    22741 aactcagccc ggtcaaaatc ccgcccaccg ccaaaagcgc ccagcccacc ggcgttaccg
    22801 aactggccgc gccggcggtt aaaatcgtgc cgcctacaga agtcgccgtg ccgcccaccg
    22861 cgcccgtcaa tgccgctttg gccgaggttt tttccgagga gcatccggaa gtgccgcccg
    22921 agaattgcac ggccgttccg gtaagcacgc cgggctcgta atccggcaac acgccctgtt
    22981 cgatggcctc gagcgtccag ttgtaatacg cgtcgttcga cggaatcccg agcgcactca
    23041 ttgccgctgc ggcattcgac gagctgggct gctgcggagg gtccttcgtc gtcgcgccga
    23101 gaaaccccag catcactcgc cgttgagcta aaaccgaatt ggaggtcaga tacattcaat
    23161 tgatgatccc cggcgcctcg acgcgatatt cgccgccggc gaaaaaaatc attttgcgca
    23221 gcttgtcgaa aatcagccgc ggcagctcgg cgctggcctt ctcttccgcg aatttgtgga
    23281 cgtactcgac cggctcgtag ccgccctggg ctttgcgcgc ttcgtacacc acgaagccgc
    23341 aatcgcccac gtcaatcaga tccttggact cgtccacgcc gaaacgcgcc aggcaatttt
    23401 ccagattctg attgccgccg atgcagtaga gctgcgcacc gtccggcgag ctcgccagag
    23461 tgatgccgtc gccttcgaaa ttgatgtgcg gctgcttctc ccaacgctgc accagcgcgt
    23521 cgccgctcgc gtccgcgcgg ccaatgccca gcgccagcaa tttgccgagc gcggtgtatt
    23581 ccatcctcac cgcagcggag acgtgcttct cgacaatccc ttgcggatct ttgccatgaa
    23641 atgcttgata tagcttcacg gcttcgctgg actcgtcctg attgcgccgg cgacgtttgc
    23701 gctttgggtt gcggtcaaaa accccacgga ccggcttggg catcttcgcg gtgagttttt
    23761 tcgcgccgcg catgtttttg cagatcggac acgagcagcc cggcttgtga ttgttaccgc
    23821 ggcgcgcgtt tttcttcacc gctttcgacg gattgccgaa aatcaccaac tccgcaggat
    23881 tcttgccggc cagagtgcag gaaggattcc tttccccctt cgggtgaagg atctcgagga
    23941 gctggccgga acctttgcgc ttgccgggcg gaaacgcgat gcgcagctca tgcgcgccgt
    24001 ggcgcttggt gcgcacacgc cagccgcgcg gccgcgtggc cacgtgctgg atgcgaaatt
    24061 gagctacgct cgccattgtt tcgcttcggg cctactgccc gtcgccctct tcgccctctt
    24121 cgttttcttc ggacggctcg tcgtcgtcgt cgatttcttc gggttcagcg tccgctgcgt
    24181 tcgaacccag atcgccgtgc tcgcccactt cgcctacgtc gccgcagcgc gcgtgatgct
    24241 tgcgctcctt cttgtccttg gacttctttt tgcccttggc ctcggatttg tcgtctttat
    24301 gcccgcgaat tttcatgctc gcctcccgga aaacttatcg cttcgaaatc cgcctataac
    24361 tcatgtacga ttgccacgcc gttccacttc tccccaaagg agcccatccc gatgaaactc
    24421 cacccggcca cttcattttt tctcggcatt ggtgttggcg ccggcgctct gttcgcgtat
    24481 ttcatgttga ttgcgtaccc caaaattaag tcagtgatgc gaggccagga agcggtggcc
    24541 accgccttta cccaaatgta cgaatcaaag agatccgccc tgctcacatg ccaagtaaat
    24601 ctgcaaaacg cgcgagtctc ctctgtgatg atcgccgaac cccaacttcc atccgatcag
    24661 gtcggcgaat ttttcggctc aattcttggt gcgtttttcg gccatcctca agccgcacag
    24721 caactcgcca cccaactcac ttccaccggc atgaaacctc ggttcttcat caacaccgcg
    24781 aactacaaaa tcggcagcta tagcacagaa gggaatgccg ccgttttcta ctactacgac
    24841 ccagcaaaac tgcaatgggt aggaccaaac gtgatcccac ccgtgaatac cctcattaca
    24901 gaaaatccgg taaaataagc gcgccggcag ctcccaaagc aacccatgcc caattctccg
    24961 agagccactc agtaaatcct gacttctgtg cggttgaagc ggcgctcgat tgcgcgaccg
    25021 cacaccatct atttgcgccg tctgtatcac ctgcagcctg ggcagcatga cactgctgtt
    25081 cggcataggc cgtattttga gccgataatg tgatgttcga ctgctgctcc tgtaacacat
    25141 caatgtactt atttatttca taagcagccg caagtaaggt tcccagggca atgatcccgg
    25201 cgccaatata tatcgcccac tgttccaagc cagtcgtatc cggagggtta ccaaaaacag
    25261 caccatacgt atagatatat gcattcaaaa tcttctcgta tcgtgccctt gctgtgttaa
    25321 ctcctggtcg aagttgattc tggtaatcct gattacccat gttggtgatc agcgactgct
    25381 caagagcaat cagcctggag tttgcctgtc gcaattgtgc tgcaattaaa ttagcctgag
    25441 acatcgtatc tggagtcgcg agctgagccg ccgaaccgat taatggcacc atcgaaagaa
    25501 cgctcatcag ctcaaaatcc tccggcgata gtggcgcatg cggcggcgac ggccgctgcg
    25561 cttgcgtccc agcaaaatca atcccgcggc gacgatggcc acgccgccaa tgagaaattt
    25621 catgttcaaa gtcgcgtcat cgaaaatcga cgagagatgc gtcaccggcc gcagcggcag
    25681 cacgttgtaa attccgtgct cgatggcctg gttcggccgt ccggagagat agcccaggtc
    25741 cggaaccgcc ggcacgttct gctggcgcag aaaattcgcc atgtccgagg gcgaagccac
    25801 gttgagcaaa taactcaccg atgcgctcct ttcttcgacg cttgcgcgat caggaaaatc
    25861 gccagcactc cgccgagcaa atatttgtta ggcacgccga gaatgggttg ctggtcgagc
    25921 cacggcgttc ctggcgccgg cggcgcagga ttcggcagcg tattgggata agccaccggc
    25981 aacgcaggcg ctccggaagg caacagcggc ggcgcgtccg cgatgccgta ggtgttcgcg
    26041 agcttcctcg gcggcgccac tacgctcaaa ggcgcgttca tggtcggctg cggcagcgga
    26101 atccccgccg gattcggcga cagctcggga ggcgaagcga aatccaccgg cgtcaatccc
    26161 gctgcgggat tgttcgacag gttcggattg ttgggcatga tgtacatcag cgcctcttgt
    26221 tcgccgcgga agccagcacc agaatccccg caaggccggc caccagatat ttgttgggca
    26281 cgcctgtgat catttgctga tcgagaaacg gcaatttcgg cccgggcgca gcagctccgc
    26341 gcatcaccgg cgccatggtg gacgaaacca ctgccggcgc agaactcacc ggcggcgtga
    26401 gcgtggtcga aatcgttggc gcgacagttt gaatctgccc ggaagtgtcc ggcaccagca
    26461 ccgcaggcaa cggattcacg taggacgtat ccgcgggcgg cgtcagatcc gttgaaatca
    26521 ctgaaggctg aatggccacg ccgtcatcgg cgagcccatc gagatagtgc ccgccgcggc
    26581 aatgacaggc caggccggaa agcgaaatgc ttcgccggcg gcgttcacga tcgccggaga
    26641 gctgcgcgcg cgatggccgc acagcgcgca aaggagtggc cggcatgaga tacatcagcg
    26701 gcccctcttt cgcgtgatca gcagatagcc gagaaatcct gcgcccagaa tccaaaaatt
    26761 tttcacgccg gagatgagcg actcggaatt gaaccagtcg atcacgctct ggtaggagct
    26821 ctccgtcacc ggcgcggcag aagtcacgct cacgctgggc gcggccgctc cctgggaaac
    26881 cagagccgcc gcagcggacc cgggcaactg cgtctcgacc cacacgcctg atgtcggatt
    26941 ccaattccac acgccgccat cggccgcgaa gtacggctgc gttttcggat agctcgccgg
    27001 cacaggagac gccagcagat tcgaataatt cgcgggcgcc ggcgcgaacg tttgcgggtt
    27061 cgcgggattc accggagaaa tcacctggcc gccgcccgcg ccggcattgc cgcaaggaac
    27121 gatcgaggaa actccgccgg tggcgggaga agactcgacc acgcacatcc ctgaagacgg
    27181 agttggcaac gaagagccgg agggcgccgt agccggagga acgggcgcaa ccaccggcaa
    27241 aatatgcact ggctgcggcg gcgccggaaa cgggaaaatg ggaaacggcc gcaccgggaa
    27301 aaattccgga atcgcctgat tatccaaaat gctgcgcgtg atcaggccca agcccgacat
    27361 cccccgcgca cgcagtcgcg gccgcacgcc acgcgcagaa gttgccggca tcaggtacat
    27421 cagcgtctcc ccatggccat cacggcgatc aagccgatgc ctacgagcag caaagtggaa
    27481 gtggagatgc cgccaaagga cgcgctggcg taaggcgaag aaaacgctcc gtagccggga
    27541 acgccggaag gcaatccgga ctgcgaagtg gtgggcatca aattgatggg attggcgcgc
    27601 gacgcggtga tgatattcga agtgccggta gtaagctcgg gaatgatctg tccccaattg
    27661 atgtcgccca tgccgccacg atagcgtccg cgctgcggct gggcttggcc ataaccgctg
    27721 gcgtaaaaat tcaatccctg cacgtccacg tattcgtcgg aataaagcga ccacacgcgc
    27781 ttgcgcgtga aacgcttcgg cccgcggcca aatgcaggat tgcgccgcgc ggcgtccacc
    27841 ggaacccatc gaccgctcag gaaaacttcc ggatagacgt gcgtgaaggg ctgctcgccg
    27901 tcgacgtcgc gcggcacgct cgagatagtg ataatccttg cgcgatgccc caccgtgccc
    27961 agcaacgcgc aaagcaaaat cgtgaagtcg tcgcagtcgc caatgcccag ccgcaggatc
    28021 tcgtcagcag agtgcagcgt ctctttaccg acgatgtcgc gggtgaagcg caccgagcgc
    28081 agcacccaca ggtagatcgc gcgcgcttcg ccggcagcgt cgaactgcgg aattttactg
    28141 gaacgcaaaa tgaaggcggc cgcttcgtgc accacgggat gcttcacgcc ctggtcaatc
    28201 agcgcgcgca tccgcgcgat ggtggcagct gtgccgtcgt cccctagcgg gatccgcgac
    28261 acggttgaag gcatcggcat cggccgaacg gcttccagat tcatcatgtg cgcttatgtg
    28321 gccgggctca ggtttgcgtg ggccccaaag gaagagcctg gcgagccctg cccggccgaa
    28381 atcaacctcg ccgatgcgca gtaagccagc caagtcactc aaagtcaaac agttcattgt
    28441 ctacaaggat tgttaacagt ggctctacaa tggcgtacca gtacctgata agttgacaca
    28501 ataaagtttg tgctgtaaag ttccgcctcg cgtggataaa gggaaaagcc caggagaatt
    28561 gcgcaatgcc acgagctgcc agtgtgtctg aagtgccgtc ggtggtcatc aaccagaact
    28621 cacccgtcac cgaacaggtc gaagagaaag cgaagaaacc gcgcatcgat ttttggccgt
    28681 atatcgcctc gctgagcccc acggactggc gcgacacgat tgtctacctc taccgcgaca
    28741 aaccggtggt cggctccaaa aacaaagaga agtatctcga cgtgctgagc gccgctttca
    28801 ccatcgacga catcaaacag cgcttcggcg gcgaagagtt ccacgccatg ctggtgaaga
    28861 acggcaaaat cctgcacact gagcatttct cgatcgaagc ggcgcccaaa ttcgactccg
    28921 cgcgcgagat tcccggcgca gacgcctcga acgccgcgat cgtggaccgc ctgctcgata
    28981 aactcgaaca gcgcgaagaa aatcccgcat cctcactgct caccacggcc atgaccaaat
    29041 cgcaggacat gatggcgcaa gcattcgaaa ccgctctcgc taaagtggcg ggcgcgagcg
    29101 ccggcggcgg tggcgcggac ctgatcaaaa ccattcaagt gctcaaagag ctgggcctca
    29161 tcggccggca ggaaaacacc gcggtcgaaa ccattcgcat gctcaaagat ttgggagtca
    29221 tcggcggcgc ggcacagcag ccgcaaaatc agtttgaaat atttcgctcg atgctggcag
    29281 tggtcaaaga actctccggc gagctgggcg agaattcgca tcccggcaac tggaaagcgc
    29341 gcctggtgga caaagctgtg gaagcggcgc catcgctcat cggacaagtg ggcagcatca
    29401 tggacaagca agcggccatc gagcgcgaga aaaccaatcg cgccaacatg atgatcaacg
    29461 cgcgcgccgc catgcctgga gcacccgcag tagcagcgcc gacgtccaca gaccacatcc
    29521 ccgttccgcc tcctgcgcca ggtgcaccag cgccagcgcc gatgcacact gtgaagttcg
    29581 accgcaacag cgctcctgca aacgtcgaag cacctgcgcc gcaaacggcc gaattaaccg
    29641 gccagccgtc gcaggagtgg gtgagccgtc gcgtggtggc ggcgatcgag gcgggacaat
    29701 ccggcgaggc ggtggtggac ttcctcgact gcatcaatcc catgatttgc cagtattttg
    29761 cgtcgctcga tgcagagggc atccgcgcga aattcgccag cgatgccatt ctctcgcaag
    29821 cggtggcgct gccgcgattc agtgaatggc tggctgaggt agttgagtac ctgcacgagg
    29881 aaggcgaaga gtcacccggc agacgaccga actagaatca aaaaagagag agccttaagc
    29941 tcccagccag ctgcacggcg agaacgaact cacatcctcc gcaaattacg aaagctcgac
    30001 ggctgcacct tggcctcttg catccccacg atcatggtcg aaataatttt gccacacttc
    30061 gcgcagctgc acatcgcgta ggccactgta cagacccggc cggccacgtt catcacgttc
    30121 tcgaaaaaat caaacaccag accctgctgc accccgagac aatacggaca gatcccgtgc
    30181 aactgctcaa ccggctgctc tcctgggtga gcgtcgttcg atgatgaatc aggctccgta
    30241 tctgtcgcca atattttttt ttcttcgtcc acttttcctc cctcaaaaca tccgatgctg
    30301 cccgttctcg tcgactaatt tttcaggcgg cttatcggct ggtttttcct ccggctgtgc
    30361 cttgggacac gtctccgtgg tgtgctcgat gaa
//
LOCUS       DATNDT010000002         8783 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4016780, whole genome shotgun sequence.
ACCESSION   DATNDT010000002 DATNDT010000000
VERSION     DATNDT010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8783)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8783)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8783
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4016780"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="VN788_00215"
     CDS             complement(<1..408)
                     /locus_tag="VN788_00215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18984.1"
                     /translation="MIIFFAIFAVFGLALFVFIAVISLAEMPRAANETAAFIAVGEML
                     KLESLSLRNAEQILNSGDYQDLRSNPALTQVAKRLRRERRDLALLWFCMLLDDLKKLH
                     RFRAFLVRSGLSVRFYEEVQIAYRLCSSVIFLNF"
     gene            complement(405..959)
                     /locus_tag="VN788_00220"
     CDS             complement(405..959)
                     /locus_tag="VN788_00220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18985.1"
                     /translation="MGLRFCVLWEIFRHTFAHVGSLNKVVSKGLATGSSIPILLSGFA
                     FWSVLFYISHDRLHYLYPALERSFGLVQAFLLAGILVAARYYRVRLGRNVWGIAIGFG
                     AFVSASTILFSLVNLSESFAPYMQVLSPLSFVSMLAVWTWALWVYAPNPAVGMNKTAA
                     ESAALVWWESWTDTLASIKRTRNT"
     gene            complement(1862..2686)
                     /locus_tag="VN788_00225"
     CDS             complement(1862..2686)
                     /locus_tag="VN788_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18986.1"
                     /translation="MKTTPGQYLKEVRQRLGMSLRDVQDASAVIAAEEGRHGFFISAA
                     HMNRIEMDVHTPSALKLFTICAVYALDMHDVMRRYGADASRLKLYRERFLRKLTRPVS
                     SEIYGTEEKMIIPIRLDPSFRWQTTTLINRVVAQWGEVPAAFLVGCNPRQRTYAYIGE
                     DDLRMQPLIRPGSLVMIDTRHRRVEQRRYENEFDRPIYFIELRNGYRCAWCEIAGSRL
                     RVIPAPASGYPVETFSLPNEAEVVGQVVNVAMRLAPAEAASAEHELRSQVQSAVAK"
     gene            3699..3959
                     /locus_tag="VN788_00230"
     CDS             3699..3959
                     /locus_tag="VN788_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18987.1"
                     /translation="MRTYIILLRRMRSNRNRLTRSAVELDIWKSAKRCTGIELLDILT
                     CSRISDAVLLCRAPDNQTIADLLDGLNGWHTEALLANSHLRC"
     gene            4203..5798
                     /locus_tag="VN788_00235"
     CDS             4203..5798
                     /locus_tag="VN788_00235"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU18988.1"
                     /translation="MPSEKALPQRSMRFASILIVLAATGFLVVALSSRLLNRVSSSAL
                     LASALAALVIAVALHVRFLLLARREQCETAAALDATEREYKSVFDNTLDGIVVFDNRG
                     VCVQANEAARALLGNGEHSLQGKPISAFFSQSGASRGSWNAFLKGENGHGEAQILRPD
                     GTAICIEYTLRANYLLGRHVAVLRDISERKRAQEALRESEERFQQMASNIQEIFWMFD
                     AGSKKVVYINRAYEIITGRSCESLCEDPRSFREAIHPEDRVRILSRLSESLEQGRFDE
                     EFRIVMPNGAVRWVWARGFPVRGPAGIIRRLVGSAQDITARKSAEREIARNLDLAEAA
                     RTEAEAFRKTSLALTENLSMDYVLDTLLASLLELVPFESAQVLLVEAETHLFLARELQ
                     NCEPGRLPPAPATLGARDSRYLMEILATRKSLLLSDTGQEEEWLGFKGFSHLRSWLCV
                     PLVTSQQILGFLSLGDTHVRAFTREHLRLAESLAIPAAVAIQNARLYEEAEIFRTELG
                     QRLVDLQQTDGIPQRTHRADRFT"
     gene            6348..7592
                     /locus_tag="VN788_00240"
     CDS             6348..7592
                     /locus_tag="VN788_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18989.1"
                     /translation="MFATMANRWEQTTDGYPFRYFSPEQVDEMLRDGARRGRQGSHST
                     IERILKHEPELQRSDLWRRIRRLKHPNRPRSRTPFRWSSEDEEVLRTGYNGGWQEKRN
                     AVSQLLKRHPEWRPHVIWQHARKNGLVQKLSKHGQERSGEAWSDEDNRVLLDLAGYKR
                     VRVIAKVLHRSEAAIRYQLAMLGESSRVHREGFARTALARDLHFSTKAIQRLISEGLL
                     EVRDPRVTRESLDCLRQAGTLEASQKDEAEGYSPAGQVLEGGTATQPEENSGARQSAK
                     LSRAKRVWAEVAASLHVTEKTVERLIARGVLKLYDPTITEESLRRFCRRYGSLIEYDF
                     LNRETREWLRSSLDWVKTSGELVSIRFAPLRKHARVVHRCGHCGRQIRGNAYFRHLNR
                     CRRLKPCRGRPAGAPLSDCDRN"
     gene            7924..8211
                     /locus_tag="VN788_00245"
     CDS             7924..8211
                     /locus_tag="VN788_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18990.1"
                     /translation="MGSRQCGQNLFGYDLICGKNQGAERNRALLALQVDLSDAASRLI
                     GVNETHTAKQVVEVYSAQVYAEGDEAAVLVPVAVSMARDHPLVQADRVAAE"
BASE COUNT         2000 a         2452 c         2403 g         1928 t
ORIGIN      
        1 aaaattcaaa aagatcaccg aactgcacaa tcggtaggcg atttggacct cttcatagaa
       61 gcgaacggac aagccgctgc gtaccagaaa agcgcgaaac cgatgcagtt ttttcaaatc
      121 gtccagcagc atgcaaaacc agagcagagc caagtcgcgg cgctcgcgcc gcaagcgctt
      181 ggccacttgt gtcaaggcgg gattggagcg tagatcctga taatcgccgc tattcaggat
      241 ttgttccgcg tttcgcagcg ataagctctc cagcttgagc atctccccta ccgcgatgaa
      301 agcagcggtc tcgtttgctg cccgtggcat ctctgccagg ctaataacgg caatgaaaac
      361 gaaaagcgcg aggccaaaca ccgcgaaaat ggcaaaaaaa atgatcatgt atttctagtc
      421 cttttgatac ttgctagcgt atctgtccac gactcccacc aaacgagagc tgcactctcc
      481 gccgctgtct tgttcatacc aactgctgga ttgggtgcat atacccacaa agcccaagtc
      541 cacacagcca acatactgac aaagctgagt ggactgagaa cctgcatgta tggagcaaat
      601 gattcactta aattgaccaa cgaaaaaaga attgttgaag cagaaacgaa agccccaaaa
      661 ccgatggcga ttccccacac gtttctgcct aagcgcaccc gataatagcg ggcggccacg
      721 agtatccccg cgaggaggaa cgcctggaca agcccaaagc tgcgttccaa cgcgggatac
      781 aaatagtgaa ggcgatcatg cgagatgtaa aacagcacag accaaaacgc gaaacccgag
      841 agcaaaatag gaatcgatga gccagtagcg aggcctttcg agaccacttt gttgagactg
      901 ccaacgtgtg caaaagtatg gcggaagatt tcccatagca cacagaaacg caaacccatg
      961 ctgaggctct cgctatccca ataccacgct ggatacaacc cgggaaacaa atggtagaca
     1021 acgaacgccc ctaaatccat caccacgagg gtgtatacaa cgtaagccag cagccaccga
     1081 tagtgcttcc aaagtctatt ctgaccaagg cgccaaataa gaacgacttc gaaaaagata
     1141 ctcaggagga aaattgtaag tgaaagagga gacataagcg gagggaattc tacctgagat
     1201 aggcatcttg aagaagggga acctagcttg gcccccccct ccctatatta tattccccta
     1261 ctcccctggc ggaaccgtcc aaggcggcgg cattccccca tccaacttga agcaattacg
     1321 gcatgttctc gcaagcgctt tcattctccg ttcctcctta aggtgaggta aaacggtgcg
     1381 cagcgtaaat cggacacctt gggtctccac catctttctg tttttgtacc gtttgcgtta
     1441 caggcgggaa gaagccaaga attgcattcc agcgagaagt tgcgtagaat ccctgccctg
     1501 atgcgcgccg aagatcaact ggtcagttac ttgagccacg cattagcggc ggtgccgaat
     1561 ccctttgagc ggctggtgta tttttcggcc ttgcgggatt cgcaaactgg acggtatctt
     1621 catgagggct ggcagacgtg ttcttcgcca gaggaagtgc atgtcacgct gcatgaagcg
     1681 catcgcaacg ctttcgagtg ggtgctaagt ttcccgcttg tggctatctc ccaggaattg
     1741 cggcgacacc ttgaatcaat tggctggtcg ggagagcggc acgcagttca gtactggttg
     1801 gagaacgagc ctttcagaga gctgattcct gacggttgtt ccgcgattgc cagaaaattt
     1861 tttatttcgc aactgcgctt tgcacttgag atcttagctc gtgctccgca ctggcggcct
     1921 ctgcaggagc aagcctcatg gccacattca cgacctggcc aaccacctca gcctcgttcg
     1981 gtaaactgaa ggtctcgaca ggatagccgg aagccggcgc gggaatcacg cgcagccggg
     2041 atccagcgat ttcacaccac gcgcagcggt agccgttgcg caattcgatg aaatagatgg
     2101 gccggtcgaa ctcattctca tatcgccgct gctcgaccct ccggtgtcta gtgtctatca
     2161 tcaccagcga cccgggacgg atcaacggct gcatccgcaa atcatcctcg ccgatgtatg
     2221 cataagtacg ctggcgcggg ttacagccca ccagaaatgc cgcaggaact tctccccact
     2281 gtgccacaac gcgattgata agagtcgttg tttgccagcg gaagctcgga tcgagccgga
     2341 ttgggatgat catcttctcc tcagtgccgt atatctcgga agagaccggt cgcgtgagct
     2401 tgcggaggaa acgctcgcga tacagtttca agcggctggc atcggccccg tagcgacgca
     2461 tcacgtcgtg catgtcgagc gcgtaaaccg cgcagatggt gaacagcttg agcgcgctcg
     2521 gagtatgcac gtccatctcg atgcggttca tgtgcgccgc ggagatgaag aatccgtggc
     2581 ggccctcctc cgccgcaatt actgcactgg catcttgcac gtcgcggagc gacatgccca
     2641 gccgctggcg tacttccttc aagtattgac caggtgttgt tttcattggc aaagacccac
     2701 cggatgaagc gtccaacgtc gggcttttct gctggtctga cctcttcgcc aaagacctcc
     2761 ctgagtaaat tttgtcgcgt ttccggaaca acagcggaag tttacttttt gcgggtatct
     2821 gatgccacat ataaattcaa tgcatctgcg atctcctggg cacgtatgct gcttcgatcg
     2881 ctgctcgact cggcgaacgt gtttcaatgt gaacacgttt ctcctctttt ttaccgcaaa
     2941 gtgttacggc aattgcccga tttatcgttt acagccaatc tgtcagattg ccggaatcat
     3001 gcagagaacc gcggaatgcg tcacaactac cctcgagata tccgagatct gccgggtagt
     3061 gcgggaggta caaaagggag gcgggcattt tcattctttt caaccgaccc gcgaagacta
     3121 tctcgtgctc ttcaatcatc cggaatcgcg gaccacgcta gccctgccga tcttagaaat
     3181 ttgtgtggag gcggtccgag aaaaaattgc atctgcacgc gaaagaattg cggacgaagg
     3241 tgcccctctg taacgggccg gcctcgcaga tttcctcgag actagcttat cggcgccgca
     3301 aagagccttc atccggactc tctgagtggt gttggcccgt tgaccggcaa cctcagatgg
     3361 tttcagaaaa tgagttgatg tattcgcagg cattcttctg ccccgcctgc atgcaccgga
     3421 gctccacatg agccgaccgg ttggccgcgt agaacacaaa aagcgaacct ccttgcagag
     3481 ctgcgaacag ccatccatta gttgtaaaac atcagttgac attctcatac gttggctatt
     3541 tactgtccga gatatacctt gaaagggtaa gtaacgtagg gagtaccgac ttgggcgctc
     3601 ggttccggcg ttgtctatcc aagtcagtgc gccttgcgtc tcgccgggga gatcctaggg
     3661 tccttcggcg ctgcagacgg attgcttggg agcgttctgt gaggacctat atcatactgc
     3721 tgcgcagaat gcggtcgaat cgcaatcgcc tgacgagatc ggctgtggaa ctggatatct
     3781 ggaagagcgc caagcgttgt accggaatcg agcttctcga catacttact tgcagccgaa
     3841 tcagtgatgc cgttctactg tgccgtgcgc ctgataacca aacaattgcc gacctgctgg
     3901 atgggctgaa tgggtggcac actgaagcct tgctggccaa ttctcatctg cgttgttgaa
     3961 atcccgtttg cctggggccg aacgcgctac accaacgttg acctgaatat gtgcgcgggc
     4021 caagctccag gcgtccgcgc gaacgagttg cggccgggca agccgcacct cccgaaaagg
     4081 catatgccgc tcaaagcgat acgcggtttc gaaacatcgc tcaacttgac gtcttgcccc
     4141 ttgaggcata gcatgaccgc tctaagcagc cccggcccga gatgttcgca gcgaggctct
     4201 ccatgccttc cgagaaagcg cttccgcaac ggtcaatgcg attcgcatcg atcctgattg
     4261 tccttgcggc aaccggcttt cttgttgtgg ctttgagttc ccgtctgttg aatcgcgttt
     4321 cctcctcggc cttgctggca tccgcccttg cagctctggt gatagccgtc gcgctgcacg
     4381 tccgcttttt gctgctcgcg cgacgcgagc agtgcgagac cgccgccgcg ctcgacgcca
     4441 ccgagcgcga atacaaatcc gtattcgaca acaccttgga tggcattgtg gtttttgaca
     4501 accgcggagt gtgcgtccag gcgaacgagg cggcgcgggc tctgctggga aacggagaac
     4561 acagtcttca gggcaagccc atatcggctt ttttttcgca atccggtgcg tcgcggggca
     4621 gctggaacgc tttcctcaaa ggggaaaacg ggcatggcga ggcgcagatt ctgcgacctg
     4681 acggcacagc catttgcatc gaatatacct tgcgggccaa ttatctgctc ggacggcatg
     4741 tggcagtgct gcgggatatc agcgaaagga aacgcgctca agaggcgctc cgcgaaagcg
     4801 aagagcggtt ccagcagatg gctagcaaca tccaggagat tttctggatg ttcgatgccg
     4861 ggagcaagaa agtcgtttac atcaacaggg cctatgaaat cattaccggc cgcagctgtg
     4921 agtcgctttg cgaggatccg cgatcctttc gggaagcaat ccatccggaa gaccgcgtgc
     4981 gcatcctttc gcgtctatcg gaatccctcg aacaggggcg gttcgatgag gagtttcgga
     5041 tcgttatgcc gaacggcgcc gttcgctggg tatgggcgcg cggcttccct gtgcgaggtc
     5101 cggcgggaat cattcggcgc ttagtgggaa gcgcacaaga catcactgcc agaaaatcgg
     5161 cggaacgcga gattgcccgc aatctcgatc tcgcggaagc ggcccgaact gaggccgagg
     5221 ccttccgcaa gaccagcttg gccctcaccg aaaatctcag catggattat gtgctcgata
     5281 cgctgcttgc gtctcttttg gagttggttc cctttgaatc ggctcaagtt ctcctggtag
     5341 aagcggaaac gcacctgttc ctcgcccgcg agcttcaaaa ctgcgaaccg ggccgccttc
     5401 cgcctgcgcc ggcaactctt ggcgcgcgag acagccggta cctgatggaa atccttgcta
     5461 cccgcaagag tcttttgctc tccgacacag gccaggagga agagtggcta ggcttcaaag
     5521 gcttttctca tctgcgttcc tggctttgtg tcccgctggt cacctctcag cagatcctgg
     5581 gctttctttc gctgggcgat acccacgtgc gagcgtttac gcgggagcat ctgcggcttg
     5641 ccgagtccct tgccattcca gcggccgtgg ccattcagaa tgcccggctt tacgaggaag
     5701 cggagatttt ccgcacagaa ctcggacagc gcttagtgga tttgcagcag acggatggaa
     5761 ttccgcaaag gacgcatcgc gctgatcggt tcacttaact gcgactccaa ctctcacgat
     5821 gaaaaaacca ccgttgccac gcaggctgat gagagatgcg cggcttgctc gtgtgctcat
     5881 ccattcggcc taatcttcgc aataagaagt gctgcgcaaa acctatccga catacttcac
     5941 gggctgcgca gcgcgcccca aatgcagact ccgggaggag cagcttcaac ggctcatctt
     6001 gcagcggtcg taattgctgc cagaactata gctcggcgcg aagtatggcg cgcggcgcga
     6061 tggcagtaca accatgaatc tattgcggat ggacttctga atttcccttc gaccaatttt
     6121 ccgaccacat cgcatatatt tcaccgcagt tcctcagctc caaagcaata tccttttctc
     6181 tcgcgtggca gcttttccga gcggcaacga agagcacctc gagatggaag gatcgaaacc
     6241 aggggcctga ccctacgttg gtcactccga tcggcacacc atctcgacaa ccaggtcgcg
     6301 cgtgaatctt agcggcgtgc ttatcgtccc tcgaccgagc cgtcaaggtg ttcgcaacaa
     6361 tggcgaaccg ttgggaacag acaacggatg gctatccctt ccggtatttc tctccggaac
     6421 aggtggacga aatgctgcgc gatggcgcgc ggcgtggccg ccagggttct cacagcacaa
     6481 ttgagcggat actgaagcat gagcccgaac tgcaaagatc cgacctgtgg cggcggattc
     6541 ggcggttaaa gcaccccaac agaccgcggt cgcgcactcc ttttcgctgg agttcggaag
     6601 atgaagaagt cctgcgcacc ggatataacg ggggatggca agaaaaacgg aacgccgtca
     6661 gccagctttt aaagcgtcac ccggaatggc gtcctcatgt aatctggcag cacgcccgaa
     6721 aaaacgggct tgttcaaaaa ctctccaaac acggtcaaga gcgttccggc gaagcttggt
     6781 cagacgaaga taaccgagtg ctgctcgatc tggcgggcta caagagagtt cgcgtaatcg
     6841 ccaaggtgtt gcaccgttcc gaagcggcca tacggtatca gcttgcgatg cttggcgaaa
     6901 gcagccgagt ccaccgggaa ggtttcgcac ggaccgccct ggccagagat ctgcatttct
     6961 ccacaaaggc cattcagcga ttgataagcg aaggcctgct tgaagtgagg gatccgcgcg
     7021 tcactcgcga atctcttgat tgtctgcggc aagccggcac cctcgaagca tcgcagaagg
     7081 acgaagctga aggatactct cctgctggac aagttcttga gggcggaaca gcgacacagc
     7141 cggaagagaa ctcgggggct cgccaatcag ccaaactttc aagagccaag cgcgtctggg
     7201 ctgaagtcgc ggcctcgctc cacgtgaccg agaagacggt ggagcgtctt atcgcacgtg
     7261 gcgttttgaa gctttacgat cctacgatta ctgaagaatc gctccggcgt ttctgtcgcc
     7321 ggtatggatc tcttatcgaa tatgatttcc tgaatcggga aacgcgggag tggcttcgaa
     7381 gctcgctgga ctgggttaag acatcaggtg aattagtttc tattcggttt gcgcctctgc
     7441 gcaagcacgc gcgagtcgta catcgatgtg gacactgtgg acgccaaatt cgcggcaatg
     7501 cctactttag gcacctcaac agatgcaggc ggctcaagcc ctgcagagga cgtcctgccg
     7561 gtgcgccact ctccgattgc gacaggaact aatcgagttc atatcagcgg cctcttagtt
     7621 cgggacccat gagaacggtg gttcaaatcc actcgccccg accacttcta atcccctctg
     7681 caaattgggt ttgtgccgtt tcacgcggcg acaggttgct ccttattttg tagcccatgt
     7741 ggcccgaata gggcaataac taccttaaag aatccacatc ggattagggc acctttttct
     7801 ttcgcttttt ccgcgcacgc tgcaacttgg cgtcagcctt gtctacggct ttcttcggga
     7861 cggcgaaaac cgctcgaatc agactatcca acgtgaatgg cttgccatcg ttgtgacttc
     7921 gaagtgggta gcaggcaatg tggacaaaat ctctttgggt acgaccttat ctgtgggaag
     7981 aatcagggcg ctgagcgaaa tcgtgctttg cttgccttgc aggttgatct ttcggacgct
     8041 gcgtcccgct tgatcggcgt caacgagacg cacactgcga agcaggttgt agaggtttat
     8101 agcgcacagg tctatgccga gggcgatgaa gccgcagtct tagtgcccgt ggcggtgagc
     8161 atggcccggg accaccctct cgtccaagcg gatcgtgttg ccgccgaata atgcgagtca
     8221 gtctacgtgc tcatggcctg ctgacccgaa accggccgct ccggagccaa gatgacggga
     8281 gataatgaga gagcggcgca cgcaaggccg atcagtggaa tcagaaacag atggtgaagc
     8341 agcctcgctt cgttccaatc gatgtcaggc tagatggcga tgtggtttgg ccgtggcatt
     8401 gttccacacc ttcgctgcaa gcgagatcta ctccttgact tggcccaacc cctagccgac
     8461 cgattaatgt gctgttgagc tgggtcccgg cagccgatca tcgttcgatt taggagggcg
     8521 ttccataggc aaggttatag attcaactct cgccgcaagc tcgctaggcc gagatctggc
     8581 aaggataatc atccccgact tgaagtcaac tcttgcaact tagtgaagcc gcgctcgatc
     8641 tcatcgattt ccagccgccg tcgaatcttt ttttgctgat ggtctcattt attgaatggt
     8701 gcggccaaca gccgtaccgt ggatctggct tgttggtagt tagtgaactg cccacggcac
     8761 caatctgcgt gcgatttata cac
//
LOCUS       DATNDT010000003         3816 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1430453, whole genome shotgun sequence.
ACCESSION   DATNDT010000003 DATNDT010000000
VERSION     DATNDT010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3816)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3816)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3816
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1430453"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /gene="purN"
                     /locus_tag="VN788_00250"
     CDS             complement(<1..494)
                     /gene="purN"
                     /locus_tag="VN788_00250"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HXU18991.1"
                     /translation="MKRRIGVLLSGRGSNFEALAASAEAGRIPNAEIAIAVSNREDAP
                     GIEKARALGIEARVIASKGLEREAYDRLVEAALREKNVDLVCLAGYMRLLSPFFVTAF
                     RNRILNIHPSLLPAFPGLEPQRQAIDHGVKFSGCTVHFVDENLDAGPIILQAVVPVED
                     DDTPA"
     gene            1137..2246
                     /locus_tag="VN788_00255"
     CDS             1137..2246
                     /locus_tag="VN788_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015328903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXU18992.1"
                     /translation="MKLNDYTTLGRTGLRISPLCLGAMGFGTGWGWGADEKLSFEMMN
                     LYLEGGGNFIDTADGYQMGQSEEIVGKYVAERKLRDRAVIATKFTFGVEQGNPNAGGN
                     SRKNIYRALESSLRRLKTDFIDLYYLHSWDLVTPVEEVISTLTDLVREGKIRYIGLSD
                     TPAWYFTRAQTLAEKGGLERVATLQLEYSLAERNIEREHIPAAQELGIGLCPWSPLAG
                     GLLTGKYKREGSGGKGEGRLTMPNPIFNKFTEHNWRVLDALLEVAKQVGKSPAQVALN
                     WLATQPGVTSIILGATKTAQLADNLGSLDFTIPAELRKRLDEVGAPEPVHPYNFYEPF
                     MQSRISGGVTVREWRRGYLYEGALAPPTEKKASAS"
     gene            2581..>3816
                     /locus_tag="VN788_00260"
     CDS             2581..>3816
                     /locus_tag="VN788_00260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU18993.1"
                     /translation="MRIPHGRSIGQKLTRVVLISCGVALLMACTALAAYDFVTFRRDL
                     AKSLIQTARATGDDTTAALSFGDAGSVREILSSLSAQNHIVQACVYPLDGTILASYVR
                     PGSHPDPLPPSPGPDGIASRPGQVILFQPIERDGERIGTIYLDSDTGELYAREWHFAE
                     IAFLVVLGSFVTAYLLAARLQRTISEPVRELAATVSAVSLRKDYSIRATKSSEDEIGS
                     LVDGFNEMLDRIHEREKSLQSARDELEIRVSERTSELQNEIAERKHTEQQLEERTAFF
                     NSLIENSPIAIVAVGMDDIVRFCNPAFENLFGYRQADVIGKSLLGLLTNSEIRAEVAT
                     NREALWKGKVIHTVSRRARSDGTLVDVEAFSVPLGPMGHRTGAVMQYQDITERKRAEE
                     ALLRAKDAAESASRAKSEFL"
BASE COUNT          828 a         1144 c         1077 g          767 t
ORIGIN      
        1 gcgggcgtgt cgtcgtcttc caccggcacc accgcttgca ggatgatcgg tcccgcgtcc
       61 aggttttcgt ccacgaagtg caccgtgcag cccgagaatt tcaccccatg gtcaatcgcc
      121 tgcctctgag gctcgagtcc agggaacgcc ggaagcaatg aaggatgaat attcaggatg
      181 cgattgcgaa atgcagttac gaaaaaaggg gaaagcagcc gcatataccc ggccaggcaa
      241 acgaggtcaa catttttctc gcggagcgcc gcttcaacca gtctgtcgta tgcctcgcgc
      301 tccagcccct tggacgcaat cactcgcgct tcgattccca gcgcgcgcgc tttctcaatt
      361 cccggcgcat cctcgcgatt gctgaccgcg atcgcgattt ccgcgttagg aattcgcccc
      421 gcctcggcgc ttgccgccag cgcctcgaaa ttcgatccgc gccccgagag cagaacgcca
      481 atgcgccttt tcatgaggca gcgaaattag cacagttccg gtagtcgtca aacgccctcg
      541 cgagccttcc cttggatagg cgtagcaatt ttggactgca ggatttttga aggagggggg
      601 cggccggtgg ggggaccggc cgctcggggc atagtgagga aggtgcagac attcggtgcc
      661 aggttggctc cgaatgctca atcatcttgc cggtgaaccc cgcgtgcgtc gatggaccgc
      721 tcggtttaat tcgacccgta ctaacgtacc gtcccttcga attcgcggca tattcgccgc
      781 ctgagggttg gtccttgatt cccccaaaaa gtccacacca cctagccctt gcactgacgt
      841 tcccgtgtac taagatggtc ccgtgaagaa cgcaatagag atcggtgaaa caggcagctt
      901 tcgtggcggc gcatgggaac cccgcctcgg cgacgtccgc agcgccaagg cgcaatgcct
      961 cacacaatca gttttctaat cgacgcaagc gtcgagtaga atctggcgta acttacagaa
     1021 aacaaagggg tggcagtaag gctactcgtc acatttcaaa aggggccgct tcttttttcg
     1081 attcgcgagt cctgtcggcg gcgggccgca tcgcatagcc tcacgggagg aattatatga
     1141 aattgaacga ctacacgacg ctgggacgta ccgggttgcg catcagccct ctgtgcctcg
     1201 gtgccatggg gttcgggaca ggctgggggt ggggcgccga tgaaaaactt tcgttcgaaa
     1261 tgatgaatct ctatctggaa ggtggcggca atttcattga taccgccgac ggctaccaga
     1321 tgggtcagag cgaggaaatc gtcggcaaat acgtagccga acgaaaactt cgcgaccgcg
     1381 ccgtcattgc cacaaaattc acattcggcg tggagcaagg gaatcccaac gcgggcggca
     1441 acagccgcaa aaacatttat cgtgccctcg aaagctccct ccgccggctc aaaaccgatt
     1501 tcattgacct ctattacttg cactcctggg atttggtcac tcccgtcgaa gaagtcattt
     1561 cgacgctcac cgatctggtt cgtgaaggaa aaatccgcta catcggactc tccgacacgc
     1621 ctgcatggta tttcacccgc gcgcaaactc tcgccgaaaa aggagggctc gagcgcgtcg
     1681 ccactttgca actcgaatat tcgcttgccg aacgcaatat cgagcgcgag cacatccccg
     1741 ccgcgcagga actcggcatc ggtctgtgtc cgtggagtcc gttggccggc ggtctgctga
     1801 ctggcaagta caaaagagaa gggagcggcg gcaaaggcga agggcgccta accatgccca
     1861 atccgatctt caataaattt accgagcaca attggcgcgt cctcgatgcg cttcttgaag
     1921 tggcgaaaca ggtcggcaag agccccgcgc aggttgcgct caactggctc gctactcagc
     1981 caggagttac ttccattatt ctcggagcca cgaagacggc gcagctcgcg gacaatctcg
     2041 gctcactcga cttcaccatc cccgcggagt tgcgcaagcg gctggacgaa gtcggcgcgc
     2101 ccgagcccgt tcacccgtac aacttctacg agcctttcat gcagagccga atcagcggcg
     2161 gcgttaccgt gcgggaatgg agacgcggat acctctacga aggtgcgctt gctccgccaa
     2221 cggaaaagaa agcgagcgcc agctagcccg ccggccgaag acggacgtct cgttccgtat
     2281 cggtctacgg tcgcatgttc cgtattgggc cggtaagccg catggaaggt cgatcaactc
     2341 gatcgtacgc ttcatctctc atatagccgg cgtagctcgc tgatttggcg ggacgtggcc
     2401 cgaatctgat cgctcccggc ttactaccag tctgtcttac aacttccgca ccgatacggt
     2461 caaagcagcc cgtttccctg ccgatagtac ccacaggagc gtagttctgt cgccagagat
     2521 gacggccaca tcgagaatgc cgctgtggcg cacgattcgg aggtaacggc gagagctgat
     2581 atgcgaatac cccacggccg atccattgga caaaagctca cgcgggtcgt cctgatcagc
     2641 tgcggagtcg ccctcttgat ggcatgcacc gcgctggccg catacgactt cgtcaccttc
     2701 cgaagggatc ttgccaagtc cctgatacag accgcgcgtg ccaccggcga cgatactacg
     2761 gccgcacttt cctttggtga cgccgggtcc gtccgtgaaa ttctgagttc gctgagtgcg
     2821 cagaaccaca tcgtccaggc ttgcgtctac ccgcttgatg gcacaatttt agcgtcgtac
     2881 gtgcggcccg gttctcatcc cgatccgttg ccgccatctc cggggccgga tggaatcgcg
     2941 agcaggccag ggcaagtgat tcttttccag ccgatcgagc gcgacggaga acggatcgga
     3001 acaatctatt tggactccga taccggtgag ctctacgcgc gggagtggca cttcgccgaa
     3061 attgcgttcc tcgtagttct tggctcgttt gtcacagcat acctgctcgc cgcgcgcctg
     3121 cagcgcacca tttcggaacc ggttcgcgaa ctggccgcga ccgtttccgc ggtttcgctg
     3181 agaaaggatt actccattag agctaccaag agtagcgagg atgagattgg atctctcgtc
     3241 gatggcttca acgaaatgct cgaccgaatt cacgagcgtg aaaaatcctt gcaatcggcg
     3301 cgcgacgaac tcgaaatcag agtaagcgag cgcacatccg aattgcagaa cgagattgcc
     3361 gaacgaaagc acacagagca acagctggaa gagcgtacgg ctttcttcaa ctcgctcatc
     3421 gagaatagcc ctatcgccat cgtcgcagtc gggatggacg atatcgtgcg attctgcaac
     3481 cctgcgtttg aaaacctttt cggctatcgc caggccgacg ttatcggcaa gtccttgctt
     3541 gggttgctca ccaactcgga aattcgcgcc gaagtggcaa ccaatcgaga ggccctttgg
     3601 aaggggaagg tcattcacac agtatcccgg cgtgcccgga gcgacgggac gctggtcgac
     3661 gtcgaagcct tctctgtgcc tttaggtccc atgggacatc gcaccggagc cgtcatgcag
     3721 tatcaggaca tcaccgaacg caagcgcgcc gaagaggcgc tccttcgcgc aaaggatgcc
     3781 gccgaatccg cgagccgcgc caaaagcgaa ttcctg
//
LOCUS       DATNDT010000004         3365 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1992626, whole genome shotgun sequence.
ACCESSION   DATNDT010000004 DATNDT010000000
VERSION     DATNDT010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3365
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1992626"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="VN788_00265"
     CDS             complement(<1..506)
                     /locus_tag="VN788_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18994.1"
                     /translation="MRNSFVPIFAAMILLATSAAAQQSADTNRQSASATSTQTNRTAD
                     GHPDLSGIWAYSIDLPGGALQKESANGSVEIEKVDLSGRHAAKIAVPGALPSTPAPSY
                     KPEYQAKVKYLFDNEAKVDEVFFCGKPGVPRISSPRKIVQLPNEVIFFYEDISGDPYR
                     IIPTDGRP"
     gene            complement(558..986)
                     /locus_tag="VN788_00270"
     CDS             complement(558..986)
                     /locus_tag="VN788_00270"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXU18995.1"
                     /translation="MRYKTSGALVLAMLFAAGAAWAHHSPSSIFDMSQKFVLTGTLTQ
                     VEWVNPHIAVYINAKKSDGTVENWKFESAPPSWLRRVGVNRSDFVKAVGQTVTVEGNR
                     ARDGSLYGFLTKVTFADGSSLAMVAGRPPQPDAAPAPAPH"
     gene            1100..1717
                     /locus_tag="VN788_00275"
     CDS             1100..1717
                     /locus_tag="VN788_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18996.1"
                     /translation="MIKAGYLALLCLLVCGVFAAQTVSIGQTSSDNSSAQQPSLPVIN
                     SGACPFEGCKFGKWIVTHSSPIFSSWKNDEKPVATLKKGAIVTALTGIHITHEPDRIL
                     IVKPIPALGLQPGDVILRYMYRGEGFADIWVKGAWKKEYDCSFITEKDGSGCSRDCSA
                     KVISDGRKDWWVRVKTAHGSIGWAKVKDQFDCMDALGGDPKCDNL"
     gene            1883..>3365
                     /locus_tag="VN788_00280"
     CDS             1883..>3365
                     /locus_tag="VN788_00280"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXU18997.1"
                     /translation="MAEPQIQRGVSLELSERLTGTIAGRFAIQSALGKGGMGEVYLAQ
                     DTKLRRPVALKRMAPRLQTDEGNRRRFLHEARFASRLSNPHIAGIYDVIEEATETLLV
                     MEYVDGETLRQRLRRPVTVDEFLPLALQCSEALAAAHELGILHLDLKPENIMLTRTGD
                     VKILDFGLARHVPRADSATTDHLTSNAGRTPAYASPEVLLEKEPDPRSDIFSLGVVFY
                     EMLAGKNPFLTDSIFQTSRRILEETQPSLRSVNSAVPQDLENIIAKMLAKDPAKRYRW
                     AAEVLADLRAVERGSSITSVAAFSPRPAGHHWLKSAAIALLILVILGAGAAAIPSVRQ
                     HVSVWLGINAIPQERQLAVLPFEVVGGDPGARAFGDGLTETLTAKLTQLTASHALQVI
                     SASEVRSNHVTTPGQARQEFGVNLILEGSLSRSGDLVRVNYSLVDTRSHRQLAADSIT
                     AAVADPFAVQDQVVNGALQMLDVVARPPELQSLDAHGTQLPAAY"
BASE COUNT          714 a         1004 c          926 g          721 t
ORIGIN      
        1 tgcggccggc cgtcagtggg aatgatccgg tagggatcgc cggaaatatc ctcgtagaag
       61 aaaatcactt cgttgggcaa ttgcacgatc tttcgtggcg aactgatgcg cggaactccg
      121 ggcttgccgc aaaagaaaac ctcatccacc ttggcttcat tgtcgaagag atatttcacc
      181 ttagcttgat attcgggttt gtaagatgga gccggcgtgg atggcagcgc gcccggcaca
      241 gctattttcg ctgcatgcct tccgctcaaa tccactttct cgatttcaac tgaaccgttg
      301 gcgctttctt tttggagcgc gccgcccggt aaatcaatcg agtaggccca aattccgctg
      361 agatcgggat gtccatcggc cgtgcggttt gtttgcgttg atgttgcgct ggcggattgt
      421 cgattggtgt ctgcgctttg ctgcgccgcc gctgaagtcg ctagcagaat catcgccgcg
      481 aagatcggaa cgaatgaatt tctcattgct tgcgctcctt tcaccgaagg atcagcgatt
      541 cggcgaatga tcaatcgtca gtgaggggcc ggagcgggcg cggcgtccgg ttgcggtgga
      601 cgtcccgcta ccatcgccaa cgagctccca tccgcgaacg taaccttcgt aagaaatcca
      661 taaagcgagc cgtcacgagc gcgattccct tccaccgtca ccgtttggcc gacagctttc
      721 acgaagtcgc tgcggttcac gccaacccgc cgaagccacg aaggcggcgc gctctcaaac
      781 ttccaattct ccaccgtccc atcactcttt ttcgcgttga tatacacggc gatgtgaggg
      841 ttcacccact ccacttgcgt gagagttcct gtcaaaacga acttctggct catgtcaaat
      901 atggaagaag gcgaatggtg cgcccacgcg gctccagccg caaatagcat cgccaggacc
      961 aatgcaccgg aggttttgta tcgcaaggcg tgtccccttt cccaattttg gcgcgagtgt
     1021 atcacagagt gccccgaccc gagctaacgc tctggataca gccgcatgag ctgggcttag
     1081 aacttggtat cgtattgcca tgataaaagc tggttatctc gcacttcttt gcttgcttgt
     1141 atgtggggtt tttgctgcac aaactgtttc tattggacag acatcctctg acaattcctc
     1201 cgcgcaacag ccatccttgc cggtcattaa ttccggcgct tgtccgttcg aaggctgtaa
     1261 attcggcaaa tggatcgtta cccacagcag ccccattttt tcatcatgga aaaatgatga
     1321 aaaacccgtt gcgacgctca aaaaaggtgc aattgtcact gccttgacgg gcatacacat
     1381 tacccatgaa ccggaccgta ttctgatagt caagcccatt cccgcgctcg ggctccaacc
     1441 gggtgacgtc atcttgcgat acatgtaccg aggagagggc ttcgcggaca tctgggtgaa
     1501 aggcgcatgg aaaaaggaat acgattgcag tttcatcact gagaaagacg gctcaggctg
     1561 ctcacgcgac tgctctgcca aagttatctc tgacggcaga aaggactggt gggttcgcgt
     1621 gaaaacggcc cacggctcaa ttggttgggc aaaggtaaag gaccaatttg attgcatgga
     1681 cgcgcttggc ggtgatccca agtgcgacaa tctctaacgt agtagcttct aacggggaaa
     1741 ttgtagtttt cggggaagaa ttgagcgtaa gatacgcgtc acgagtcggg cgacatcccc
     1801 atgccgaggc gcggcaatct gaacctgttc gctcttctcc ccaagctgga cccactatcc
     1861 tgattgactg gctacggacc caatggcgga gcctcaaatt caacgcggcg tctctttgga
     1921 gctgtcggag cgcctgacgg gaaccattgc cggccggttt gccatccagt cggccctggg
     1981 caagggtgga atgggcgagg tttatctggc gcaggatacc aagctgcgcc ggcccgtggc
     2041 tctgaaacgg atggcgccgc ggttgcaaac cgatgaagga aatcggcggc gattcctgca
     2101 cgaagctcgg tttgcttcca gactcagcaa tccgcacatc gccggtatct acgatgtaat
     2161 cgaagaagcc accgaaacac tcctggtgat ggaatacgtc gacggggaaa cgctacgcca
     2221 gcggctccgc aggccggtca ccgtggacga atttcttccg ctcgcgctcc aatgcagcga
     2281 ggcgctcgcc gcggcccacg aactcggcat cctgcacctt gacctcaagc ccgaaaacat
     2341 catgctaacg cgcacgggcg atgtgaaaat tctcgatttc gggcttgccc ggcacgtgcc
     2401 ccgagcggac tccgccacca ccgatcacct cacctcgaat gcgggccgca caccagctta
     2461 cgcttcgccg gaagtgctgc tcgaaaagga acctgatcct cgaagcgata ttttctccct
     2521 cggcgtggtt ttctacgaaa tgctcgccgg gaaaaacccg ttccttaccg acagcatttt
     2581 tcaaacttcc cggcgcatcc tcgaagaaac gcaaccatcg ctgcgctccg tcaattcagc
     2641 agttccgcag gatctcgaaa acatcatagc gaaaatgttg gccaaggatc cggcgaagcg
     2701 ctatcgctgg gcagccgaag tattggcgga cttacgcgcc gtcgagcgcg gcagctcgat
     2761 aacgtccgtc gcggcgttct cgccgcgccc cgcgggccac cactggttaa agagcgccgc
     2821 catcgctctg cttatcttgg tcattctggg tgctggcgcg gcggcaatcc cttcggtccg
     2881 ccagcacgtc agcgtgtggc tcggcatcaa tgcgatcccc caagagcgtc aacttgccgt
     2941 gcttcccttc gaggtagtag gcggagaccc gggcgcgcgc gccttcggtg acggcctcac
     3001 cgaaactctg actgcgaagc tcacgcagct taccgcgagc cacgcactgc aagtgatctc
     3061 cgcatcggaa gtgcgttcga accacgtcac cacaccgggc caggcgcgcc aggaatttgg
     3121 cgtgaacctt attctcgaag gaagtctgag ccgttcgggc gatctcgtgc gcgtcaatta
     3181 cagcctcgtg gatacgcgca gccaccgcca gctcgctgcc gatagcatta ccgcagcggt
     3241 agccgacccg tttgcggttc aggatcaagt cgtgaacgga gcgctccaaa tgctcgatgt
     3301 cgtcgcgcgc ccgcccgaac tgcagtcgct cgacgcgcat ggcacccagc tgcctgccgc
     3361 ttaca
//
LOCUS       DATNDT010000005         4807 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_559644, whole genome shotgun sequence.
ACCESSION   DATNDT010000005 DATNDT010000000
VERSION     DATNDT010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4807)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4807)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4807
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_559644"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1794
                     /locus_tag="VN788_00285"
     CDS             <1..1794
                     /locus_tag="VN788_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXU18998.1"
                     /translation="GGATFRQIHGWDIPLRLDNPSLNLAGTLDWDPASPFVQFTNIAL
                     DAPNSRARASGRILWNRSGEKSPGRLQPPDEIALSSARIDMRDVLAWIRAFHTGIADS
                     LAVHGSAQVQAKFAGWPPHVVNAEAQTDAITVGTTGVSRTARLEPVTFKFVRGKTATL
                     AANVAWGDPHDPDGTFRLEASFRPAVVGLAGWRITGRAAQARDLIAGASAIGWNISGG
                     WDLAGPFACDLKWNDIPYSWSLPRLRSSIGAETLQPAGWLEIGGTTAASAGAEVRVPF
                     LNQPIVHIKARAELKPGSRRLSVSSAQAFGAEWTGTFNRRAPDGEWQFAVSTDHLTTA
                     DLDRWLNPAWKESFLDRMLPFLSARPAAVAPENIRAAGRIAVGELVLPPMHVSHLQGN
                     LQLDGRQIALSNASGQFYGGRLGGSVDAILSAVPVYRTSFDFSDVSLSALASASPKLA
                     KLLSGSASGQIWVEAHGASRAGLIGSLTCQGRARLANAELRRFDISLPLGGSAENGST
                     LFPDGSATFSCAQGKVELHKLDLLSGVGGWIQGAGSVDFNRNIDLRLRALSTLPDNPD
                     QQLAAFHLGGSLAAPQVSLVPAAPVHRSRQK"
     gene            1855..2826
                     /locus_tag="VN788_00290"
     CDS             1855..2826
                     /locus_tag="VN788_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU18999.1"
                     /translation="MSVFVAYFCVMALAIGAIADQRVHLNPKLAAGDLLRYKIDSGTS
                     TTSAITTPLANPEGGSQSNQAIHLLVRLDVLGATAASDSVRGGVRLRATFEKSSASSE
                     NDAVDPSTPSLASRYARIEGHAIEFTLAPDGQLTGFTGLKEIFPDSAAAQPIISWLSA
                     LSPDAGMPAKGVRIGEKWKSEHPFQGQPLSDLLWRTESIYLRNEPCNSATVPAPRSNR
                     PATSASGCAVILTHFEISRQGSARSDATPDDYRRNGLRTSGTWLGSGEALESISLATG
                     LLVSSTQTSKQDADYEVVSVATGSRIHEQGHVQSQSEITLITAPKLP"
     gene            2930..3451
                     /locus_tag="VN788_00295"
     CDS             2930..3451
                     /locus_tag="VN788_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXU19000.1"
                     /translation="MLVTRQAVLPDVPGIHGIIQPYADEGTLLPRSVAELSENVRDFI
                     VAEEEGRILGCGALHLYGTHLAEIRSIAVSPQGKGRGVGRALVEALMEDSRRHGVTCV
                     CLFTRAPGFFAHLGFEIAKREELPDKIYKDCLHCPKLMDCDETAMVIGKIPSNTNGLH
                     DSRILIPLVLPKS"
     gene            3544..>4807
                     /locus_tag="VN788_00300"
     CDS             3544..>4807
                     /locus_tag="VN788_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HXU19001.1"
                     /translation="MAAKFKLASDYDPRGDQPEAISEIVRGLNDGDRHQVLLGVTGSG
                     KTFTMAKVIEEVNRPAIVLAHNKTLAAQLFHEFKSFFPQNAVEYFVSYYDYYQPEAYV
                     PASDVFIEKEATINDELDKLRMSATRSLFERRDVIVVASVSCIYGIGSPEAYYGMMLM
                     LEKGQSIAREAILRKLVEIQYDRAEDLRRGTFRVRGDTIEVYPPYDDFALRVELWGTQ
                     IEAIRKIDPLSGEIRSRDGEITRLPIYPKSHYVLPAAQRERAIQTIYEELDWWKDELV
                     RQGKIVESQRVVQRTHFDIEMMRTIGYCHGIENYSRHLSGRLPGEAPPTLFDYFPTDF
                     LLVVDESHQSIPQVRGMYHGDRSRKQTLVDYGFRMPSALDNRPLTFEEFEHRVNQAVY
                     VSATPGPYELTKSGGVVAEQVIRPTGLID"
BASE COUNT          968 a         1394 c         1381 g         1064 t
ORIGIN      
        1 ggtggcgcga cctttcgcca gattcatgga tgggatatcc cgctccggct tgataatccg
       61 tcgttaaatt tggccggtac cctcgattgg gatcccgcct cgccctttgt gcaattcact
      121 aacattgcgc tggacgctcc gaactcgcgc gcgcgggcat cagggcgcat tctttggaat
      181 cgctccggcg aaaaatcccc gggccgccta caaccgccgg acgaaatcgc gctgtcgtcg
      241 gctcgaatcg atatgcgtga tgtgctcgcg tggatccgcg cctttcacac cggaattgcc
      301 gatagccttg cggttcacgg aagcgctcaa gtgcaggcga aatttgcggg gtggccgccg
      361 catgtcgtca atgccgaagc acagacggat gcgatcacgg tgggaactac gggtgtctct
      421 cgtacggctc gcctggagcc ggtgacattt aaatttgttc gcggaaagac ggctacgttg
      481 gccgccaatg ttgcgtgggg agacccgcac gatcccgatg gcacattccg gctggaggcg
      541 tcgtttcgac ccgctgttgt gggcttggca ggctggcgca tcaccgggag ggcggcccaa
      601 gcacgcgatc tgattgcagg agcaagtgcg attggctgga acatttccgg cggctgggat
      661 ttggcaggtc cattcgcctg cgatctgaaa tggaatgata ttccctacag ttggtctctt
      721 ccgcgattaa gaagttcaat cggtgctgag acgcttcagc cagccggctg gcttgaaatc
      781 ggcggcacaa ccgccgcgtc cgctggtgca gaggtacggg tgccttttct gaatcaaccg
      841 attgtccaca tcaaggctcg cgcggagttg aaacccggta gcaggcgtct gagcgtatct
      901 tccgcacaag ctttcggggc ggagtggacc ggcactttca accgtcgcgc gccggatggc
      961 gaatggcaat tcgccgtttc gacggaccac cttactacgg ccgaccttga ccgatggctg
     1021 aaccccgcgt ggaaggagag ttttctcgat cgcatgcttc catttttgag cgcacggcct
     1081 gccgcggttg cgcccgaaaa cattcgcgca gcgggccgaa ttgcggtcgg tgaacttgtt
     1141 ctgccgccta tgcatgtttc tcacttgcag ggaaatctgc aacttgatgg acgccagatt
     1201 gcactctcca atgcatctgg tcagttttac ggcgggcggc ttggcggttc ggttgatgcg
     1261 attctgtctg ccgtgccggt gtaccgcacg agtttcgact tttccgatgt gagcttgtcc
     1321 gcgctagctt ccgcatctcc taaacttgca aaattgttga gtggttcggc ctccggtcaa
     1381 atttgggtag aggcgcatgg tgcaagtcgc gccggcctga tcggctcgct tacctgccag
     1441 ggccgcgcac gcttggccaa tgccgaattg cgaagattcg atatttcact gccgttggga
     1501 ggatcggccg aaaatgggtc cacgcttttt ccagacggct cggcgacgtt ctcgtgcgcc
     1561 caaggcaaag tcgagctcca caaattggat ttgctttccg gagtaggcgg ttggattcaa
     1621 ggagcaggca gcgttgattt caatcgcaac atcgatttgc gtttgcgcgc attatccacg
     1681 cttcccgata atcccgatca gcagctggct gcgtttcatc ttggaggttc gctggccgca
     1741 cctcaggtct cgctggttcc ggccgcccca gtgcaccgct cgcggcaaaa atagtgcatg
     1801 aatgcgggta tacattctcg atcgaaccgt acagccgcgc ggttggtacg gcttttgtct
     1861 gttttcgtcg cttatttttg cgtgatggcc ttggcgatcg gtgccattgc cgaccagcgc
     1921 gtccatttaa acccaaaatt agcggccggc gatcttctcc ggtacaaaat cgactctggc
     1981 acaagcacga ccagcgcaat tacaactccc ctggcgaatc ccgaaggagg ttctcaatcg
     2041 aatcaggcga tccacttact tgttaggctg gacgtgcttg gcgcgacggc cgcttcggac
     2101 tctgtgcggg gcggcgtgcg ccttcgcgcc acattcgaga aatcgagcgc ttcatcggaa
     2161 aatgacgcgg tcgatccctc tactccttcg ctcgccagcc ggtacgctcg catcgaaggg
     2221 cacgctattg aattcacact cgcacccgat gggcaactta cgggcttcac gggcttgaaa
     2281 gaaatttttc cggacagcgc ggccgcgcaa ccgatcattt cgtggcttag cgcattgtca
     2341 ccggacgctg gaatgcccgc gaagggcgta cgcatcggcg aaaaatggaa aagcgaacat
     2401 ccgtttcaag ggcagccgct ctctgacttg ctctggcgca ctgaatcaat ctacctgcgc
     2461 aacgagccgt gtaattcggc taccgtgcca gccccgcgct caaatcggcc agcaacgagc
     2521 gcgtccggct gcgccgtgat tttgacgcac tttgaaatat cgcggcaagg ctcggcgcgt
     2581 tccgatgcta ctccagacga ctaccgccgc aacggattgc gcacttcagg aacttggctc
     2641 ggttcgggag aagcgctcga atcgatttcg ctggcgaccg ggttgcttgt cagctcgaca
     2701 caaaccagca aacaggatgc cgactacgaa gtagtgagcg tggccaccgg ctcgcggatt
     2761 cacgagcaag gacatgtcca gagccaatcc gaaatcacgc tgattaccgc gcccaagctc
     2821 ccgtgagacc ctagcgagtc gctgctgttt cggaacttct gttgcgtcca atgcgcggga
     2881 cggcgaatag atatacactc tggtgcataa atatactatg atattccaaa tgctcgtaac
     2941 gcgacaagcc gtgttaccgg atgtcccggg aattcacgga atcatccagc cctatgccga
     3001 cgaaggcact ctgcttcctc gaagcgtggc ggaactttct gaaaacgtcc gcgatttcat
     3061 cgttgctgaa gaggagggcc ggattctcgg atgcggcgcc cttcatctct acggaacgca
     3121 cctcgcggaa attcgttcca tcgccgtctc tccgcagggc aaagggcgag gcgtgggacg
     3181 agcgctggtc gaggctttga tggaagactc gcggcggcac ggcgtcacct gcgtttgcct
     3241 tttcacgcgc gcgcccggct ttttcgcgca tttgggcttc gaaattgcaa aacgcgaaga
     3301 actgccggat aaaatttata aggattgcct gcattgtccc aagctgatgg attgcgatga
     3361 aacggccatg gtgattggca agattccgtc caatacaaat ggattgcacg attcgcggat
     3421 tttgattccc ctcgtactgc caaaatccta aagagcaggg gacaccctcg cgctcccgcc
     3481 tatagacagc tgcggtataa ttgtagtttt acgcgcttcg cgcattttgc gcctggaggc
     3541 actgtggcgg cgaagttcaa actggccagc gactacgatc cgcgcggcga tcagcccgag
     3601 gcgatttcgg agattgttcg cggcctgaat gacggcgatc ggcatcaggt gctcttgggc
     3661 gtaaccggct cgggaaaaac atttacgatg gccaaggtga tcgaagaggt caaccggcct
     3721 gcgattgttc tggcgcacaa caaaacgctg gccgcccagc tattccacga gttcaaatcg
     3781 ttcttcccgc aaaatgctgt ggagtatttc gtcagctact acgattacta ccagccggaa
     3841 gcgtacgtgc ccgcgagcga cgtcttcatc gaaaaagaag cgaccatcaa tgatgagctc
     3901 gataaattgc ggatgagcgc gacgcggtcg cttttcgagc gccgcgacgt gatcgtggtg
     3961 gcctccgttt cgtgcatcta cggcatcggc tcgccggaag cgtattacgg catgatgctg
     4021 atgctggaga agggccagtc gatcgcgcgc gaagcgatcc ttcgcaagct ggtggaaatt
     4081 caatacgacc gcgccgagga tcttcgccgc ggaacctttc gggttcgcgg agacacgatt
     4141 gaggtctatc cgccctacga cgatttcgcg ctgcgcgttg agctatgggg cacccagatc
     4201 gaggcgatac gcaaaatcga tccgctcagc ggagaaattc gctcgcgcga cggcgaaatt
     4261 acgcgcctgc caatttatcc gaagtcgcac tacgtgctgc ccgccgcgca acgcgagcgc
     4321 gcgattcaga ctatctacga agagctggat tggtggaagg acgaactcgt gcggcaggga
     4381 aaaatcgtgg agtcgcagcg cgtggtgcaa cgcactcact ttgatatcga aatgatgcgc
     4441 accatcggct actgccacgg catcgagaat tattcccgcc atctttccgg ccgtctcccg
     4501 ggcgaagctc cgccgactct cttcgattat ttccctaccg attttttgct cgtcgttgac
     4561 gaatcgcatc aatccattcc ccaggttcgc ggcatgtatc acggagaccg ctcgcgcaag
     4621 caaactctcg tcgattacgg tttccgcatg ccttccgctc tcgacaatcg tccgctcaca
     4681 ttcgaggagt tcgagcatcg cgtcaaccaa gccgtttacg tttccgccac gcccggtccc
     4741 tacgaactca cgaaatccgg cggcgttgtc gccgagcagg tgattcgccc cacgggcttg
     4801 attgatc
//
LOCUS       DATNDT010000006         7147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_557858, whole genome shotgun sequence.
ACCESSION   DATNDT010000006 DATNDT010000000
VERSION     DATNDT010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_557858"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1072
                     /locus_tag="VN788_00305"
     CDS             <1..1072
                     /locus_tag="VN788_00305"
                     /inference="COORDINATES: protein motif:HMM:NF020260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutL"
                     /protein_id="HXU19002.1"
                     /translation="FLDLPYQEVDVNVHPAKIEVRFRHPQFVHDFTRDALRQALSTAR
                     PIPGFPAAKAATAGIGGSPPARSGADFAPSSEGARRFAGTPGGTGIAGPPRAEIPASF
                     ASSAAIAGDFELSGAPDRPVAQRLGFDSSAAMNFPLGATATEGSVEPEPLANAPGALP
                     SAEEIADLKPLGQVSASFIVAVNSEGLWIIDQHVAHERVLFEQHLQARREGELGGQRM
                     LLPVMVELNPRQLVTFEQIAEELRANGFEAELMGARTVAIQAAPAGIASGDAEKLLFE
                     ILDGIARENQAISIDSLQSKIAASTSCHAAIKVNTPLDKTKMEWLLGELAKTEYPMSC
                     PHGRPVVLRYSTRDIERAFKRI"
     gene            1073..1780
                     /gene="cmk"
                     /locus_tag="VN788_00310"
     CDS             1073..1780
                     /gene="cmk"
                     /locus_tag="VN788_00310"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HXU19003.1"
                     /translation="MTRKLIIAIDGPVGSGKSTLARRVATMMGYIYIDTGAMYRAMAL
                     KAQRRGISLDAQDELTALAGETRIDLRANDATQQVFLDGEDVTGAIRTSDVAQAASKV
                     AVVPGVRRVLVAEQRRAGERGGVVMEGRDIGSVVFPDAHLKIFLTASPEIRAERRWLE
                     HQQKGDAIDLARTLVEIHERDRRDREREISPLVRAPDAVVVDSTAMEAEEVARLVVML
                     AQENAKEEEMKLDSAAE"
     gene            complement(1799..3349)
                     /locus_tag="VN788_00315"
     CDS             complement(1799..3349)
                     /locus_tag="VN788_00315"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXU19004.1"
                     /translation="MKRAIPMKDSGTAVRCEARTSFWSNRILIAAVVGILFLTLYPFR
                     FSLFRHGPAFLLAGWGKDLEALDVFLNILLFVPFGFGLAERLRESGRSKAAILGVTLV
                     AGALISYMVELLQFYIPMRDSGWADVVTNSTGSALGSLVYEFAGAAILSFASLVESAF
                     VSWLNWRRVAVGLLLYVVAWLAVSIHFQREVRITDWAPHSMLLIGSSAAEEPASAWSG
                     KVFEVEFWSSALPEEVAKAITEGRPQKGPPPLASYNLVGTTFFQDRRQVPPGLTWVRD
                     SSASAPPIAEASWLMSRAPISSLVSQLARTNRFSLHVLCQASPAAARNRRVVLIAELP
                     RSINLELSQKGTHLNFWFRNHLSAKRPRLTWDVPNVFAPDQVRNLLISYDGSRLTLYV
                     DGRKEGRPYQLGPGAGLASLLHGIKSGELEGYHDIFYAIVFFPAGCLLGLAWGKLASQ
                     TGLCLAVMLLGVLGVPILFEVCRAFGGNASIWLGNIVLSILLVLAGGFWVNADRRRPA
                     SRSAAGLS"
     gene            3628..5229
                     /locus_tag="VN788_00320"
     CDS             3628..5229
                     /locus_tag="VN788_00320"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXU19005.1"
                     /translation="MTSAVGNTGLGILAMVLVSVILSGCGTDATSGARRALVASSSAV
                     SFGEVLVGSSAAQSVTVSNTGDTSIHISRATVSGKGFQLMGGTPSGEMAAGESKSLQL
                     QFAPTSFGTTDGVVTLVSDGPDPALRISLRGTGARPGLNISPGLLNFTDVPVGQSRTE
                     NIALTNSGTGSVAVNPATIAGAGFTLSGLTSPQKLAPGQTILVAVKFAPTGPGAASGA
                     ISIGSNTANSPGTISLSGVGLQALVSASATRVSFGKVVVGNTNTQPITLHNSGNAALT
                     FSQLAASGAGVSLTGLSTSTAIAPNSSATFNAVFTPASSSAVSGSITLVTNGVPSPLV
                     IDVTGTGSAASTSLGVSPASLNFGTVTPGTSRSLTSTLTNTGNSNINISSVAVTGAGF
                     SASGVSSGTVLMPGQSAALMITFSPASTGSANGARVTIASNAVNSPAIVGLTGAGQAP
                     TSHTVALSWNPTSSGGVAGYNVFRASTSGGYGTNPLNPVPVSVPAYEDSSVTGGQTYF
                     YIVTTVGAGVSSAASNEVAASIPAQ"
     gene            5551..6138
                     /locus_tag="VN788_00325"
     CDS             5551..6138
                     /locus_tag="VN788_00325"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXU19006.1"
                     /translation="MAVAGCGVGPSLVPSSTKVDFGNVAVGGSTSQLITLTNMGKMNV
                     NIARVSATGKGFSVSGRSNVVLEPNQAVTVSVGFNPAAKGKAHGALSVSSDASGGRLL
                     RIALSGSGDATSGNHTVALSWQASSSPTIGYFVYRSSNGSGVSKLNSTLNESTSFTDS
                     TVASGETYQYAVTSVSSSNVESAASSPITVTIPSP"
     gene            complement(6176..>7147)
                     /locus_tag="VN788_00330"
     CDS             complement(6176..>7147)
                     /locus_tag="VN788_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HXU19007.1"
                     /translation="VLESTTYPGTTEELVLPILAAGGLRCPIDGGLSGKEVATDFYLA
                     FSPEREDPGNKNYGLAQIPKVVGGINPASGQAATALYAQIVSKVVPVSSTRAAEMVKL
                     LENIFRCVNIALVNELKQLSLRMGLDIWEVINSAATKPFGFMPFYPGPGLGGHCIPVD
                     PYYLSWKAREYDFATRFIELAGEINTEMPYHVVNALAEALNEREKSIKGSRILLLGVA
                     YKKDVDDLRESPSLKLLELLTQRGALVDYNDPYFPALFKMRHYDFSNKRSVELTPERL
                     ASYDCVLIATDHSKYDYDAIVRESALVIDTRNATRQAKAGREKIVHC"
BASE COUNT         1657 a         2000 c         2121 g         1369 t
ORIGIN      
        1 attcctcgac ctgccgtacc aggaagtgga tgtgaacgta catccggcga agatcgaggt
       61 gcgattccgg cacccgcaat ttgtccacga cttcacgcgc gatgctctgc gccaggcgct
      121 ttcgactgcg cgaccgattc ctgggtttcc ggcagcaaaa gctgcgacag caggcattgg
      181 tggcagccct ccggctagaa gcggcgccga tttcgcgccg tcatcggaag gcgcacgaag
      241 atttgcaggc actccgggtg gcaccggcat cgcagggccg ccgcgagccg aaattcccgc
      301 gtcctttgct tccagcgccg cgatcgccgg ggatttcgaa ttgagcggcg ctcctgacag
      361 gcccgtcgcc cagcggctcg gtttcgattc aagcgcagcg atgaatttcc ctctgggcgc
      421 aacggcgacc gaaggttctg tcgagcccga gcctcttgca aacgctcctg gagcgcttcc
      481 gagcgccgag gaaattgcgg atctgaagcc gctgggccaa gtgagcgcca gtttcattgt
      541 cgccgtgaat agcgaaggac tgtggatcat cgaccagcac gtagcgcacg agcgcgtttt
      601 gttcgagcag catctgcaag cgcggcgcga aggcgagctt ggaggacagc gaatgctatt
      661 gccggtgatg gtggaattga atccgcggca gcttgtgacg tttgagcaga tcgccgaaga
      721 gctgcgcgca aatggcttcg aagcggaatt gatgggcgcc cgaaccgtgg cgattcaggc
      781 cgcgcccgct ggaatcgcca gcggcgacgc ggaaaagctg cttttcgaaa tactcgacgg
      841 aattgcgcgt gaaaatcagg ccatctcgat cgattcgctg caatcgaaga tcgccgcttc
      901 cacttcctgc cacgccgcaa tcaaagtgaa tacgccgctc gacaaaacga aaatggaatg
      961 gctgctgggg gagctcgcga aaaccgaata tccgatgagc tgcccgcatg ggcgccctgt
     1021 ggtcctgcgc tattcaacgc gcgacatcga acgcgcgttc aaacgcattt aaatgacccg
     1081 gaagctgatc attgcgattg acgggccggt aggctcgggg aaaagcacgc tggcgcggcg
     1141 ggtggcgacg atgatggggt acatctatat cgatacaggc gcgatgtatc gcgctatggc
     1201 gctgaaggca cagcggcgcg gcatttcctt ggatgctcaa gacgaactga cggcacttgc
     1261 tggcgagacc cggatcgact tgagggcgaa tgacgccacc caacaggtgt ttctcgacgg
     1321 cgaggatgta acgggcgcta tccgcacgtc agacgtcgca caggcggcgt cgaaagtggc
     1381 cgtggtgccg ggcgtgcgaa gagtgctggt agccgagcag cgccgcgccg gggagcgggg
     1441 cggcgtggtg atggaaggac gcgacatcgg cagtgtggtc tttcccgacg cgcatctaaa
     1501 gattttcctg acggcgtcgc ccgaaattcg agccgagcgg cgctggcttg agcatcagca
     1561 aaaaggagat gcgatcgact tagcgcgcac gctggtggaa attcacgagc gagaccgccg
     1621 cgaccgcgag cgcgaaatat cgccactggt ccgcgcgcct gacgctgtgg ttgtggacag
     1681 cacggccatg gaggcggagg aagtggcgcg gctggtggtg atgctggctc aggagaatgc
     1741 caaagaagaa gaaatgaaac tcgactccgc ggccgagtaa gagaagggag tatcgttttt
     1801 aggatagccc tgccgcgctt cggcttgcag gccggcgacg atcagcattg acccaaaagc
     1861 caccggcgag aaccagcagg atcgagagaa cgatgtttcc gagccagata gaagcgttcc
     1921 ctccaaaggc acggcaaact tcaaagagaa tcggcacacc caacacgccc aacaacatga
     1981 ccgcgagaca cagaccggtt tgcgatgcga gttttcccca agcaagtcca aggagacagc
     2041 ctgctggaaa gaaaactatc gcatagaaga tgtcgtggta cccctcaagt tcgcccgatt
     2101 tgatcccatg cagaagggac gccaggcccg ctccagggcc cagttgatac ggtcggcctt
     2161 ctttccttcc atcgacgtag agggtcagcc gagagccgtc gtacgaaatg aggagatttc
     2221 gcacttggtc gggagcgaag acattcggga cgtcccaggt aagcctcggc cgctttgcgg
     2281 agaggtgatt gcgaaaccaa aagttgagat gggtgccttt ctggctcagc tccaaattga
     2341 tagagcgcgg gagctccgca atcagaacca cgcggcgatt cctggcggct gccggactgg
     2401 cctgacagag cacgtgcaac gagaatcggt tggtacgggc aagctggctc accaacgagg
     2461 agatcggcgc gcgagacatt aaccaagatg cttcggcaat cggaggagca gacgcggacg
     2521 agtcgcgcac ccatgtgagg ccagggggaa cctggcgtcg gtcttgaaaa aaggtggttc
     2581 ccacgagatt gtaagaagca agcgggggag gacccttttg ggggcgaccc tcggttatgg
     2641 ctttggctac ttcctcagga agggcgctgc tccagaactc aacttcaaat acctttccac
     2701 tccacgcaga ggcaggctct tccgcggccg agctgccaat cagcaacatc gagtgtggcg
     2761 cccaatcggt tatgcgaact tctcgttgga agtggatgga gaccgcaagc cacgccacca
     2821 cgtagaggag caaccccacc gctacccgcc gccagttgag ccaggaaacg aaagcagatt
     2881 cgacgagtga tgcgaagcta agaatcgcgg ctccagcgaa ctcatagacg agggatccga
     2941 gcgccgaacc cgtggaattg gtaacaacat cggcccagcc tgaatcgcgc atcggaatgt
     3001 agaattgaag aagctcaacc atgtaggaga tcagcgcgcc cgcgacgagt gtgacgccga
     3061 gtatcgcggc ttttgacctg ccactctcgc gaagacgttc agcaagtccg aaaccgaagg
     3121 gaacaaaaag cagaatattc agaaagacgt caagcgcctc gaggtctttg ccccagccgg
     3181 ctagcaagaa tgcaggacca tgcctaaaca aactgaacct gaacgggtag agagtaagga
     3241 agagaatgcc gacgacggcg gcgatcagaa ttcggttgga ccagaaagaa gtgcgcgcct
     3301 cacatcgtac cgcagtcccc gagtctttca tcggaatggc gcgcttcact ttcgattagg
     3361 aaaaatcctg cgaaacaccc ttccttcgca gcgtaggccg acgctgcaac aactgtaaat
     3421 ccgcgcttca taatgagtct agcacggcag cgcggaacaa cgcgtttgca ctttagattt
     3481 ctggaaatac gggccatcga gaagcgaagt tttgggaaaa aaagtacaag tactggtggc
     3541 tattcgaaca ggtagttaac acctgccctt aatttggtcc ctcgttcagt agatacgcat
     3601 ttcggtgata tctaaaatgt gtccgccatg acatcagctg tggggaacac gggcctgggc
     3661 attctagcga tggtcttggt tagcgtaatc ctttcgggct gcggcacgga cgcaacatca
     3721 ggcgccagaa gagctctggt ggccagttcg agcgcagtaa gtttcggcga ggtgctggtc
     3781 gggagcagcg cggctcaaag cgtcacggtc tccaacacgg gagacacgag catccatatt
     3841 tcgcgtgcca cggtcagcgg caaaggtttt cagctgatgg gtggaacccc ttctggggaa
     3901 atggcggccg gcgagagcaa gagcttgcag ttgcagtttg ctccaacatc attcggcacg
     3961 accgatgggg ttgttacctt agtcagcgac ggaccggacc cggcactcag aatctcactc
     4021 cgcggaacgg gggcgcgacc cggtctaaac atttcgccag gcttactgaa ttttacagac
     4081 gtgccggtgg gacagtcacg tactgagaat attgccctca cgaacagcgg gaccggcagc
     4141 gtggctgtaa atccggcgac gatcgcggga gcagggttca cgctgagcgg cctcacctcg
     4201 ccgcaaaagc ttgccccagg gcaaacgatt ctggtggcag tcaaattcgc ccccaccggg
     4261 cccggcgccg caagtggcgc catttccatt ggcagtaata cggctaattc accgggcaca
     4321 atttcgttga gcggagttgg cttgcaggca ttggtatcgg ccagcgcaac gagggtcagc
     4381 tttggaaaag ttgtggtggg aaacaccaac acccaaccga taacgctcca taatagcggc
     4441 aatgctgcgc tgactttttc gcagttggcg gcgagcggag caggagtcag cttgacagga
     4501 ttatcgacgt ccacggcgat tgcgcccaac tccagtgcga cgttcaatgc agtattcacg
     4561 cccgcgtcgt cctcggccgt cagcggctca attacgctgg tcacaaacgg cgttccgtcg
     4621 ccgctcgtga tcgacgtgac cggaaccggc tcagcggcga gcacttcgct cggtgtaagt
     4681 cccgccagtt tgaatttcgg cacggttacg cccggcacca gcaggtcgtt gacttcaacg
     4741 ttgacgaata cgggaaactc caacataaat atttcgagtg ttgcggttac cggcgccggc
     4801 ttcagtgcca gcggagtctc gagcggtacc gttctaatgc cgggacagtc ggccgcattg
     4861 atgatcacgt tctcgccagc tagcaccgga agtgcgaacg gcgcgagggt gacgattgcc
     4921 agcaacgccg tcaattcccc agcaatcgtg ggcctcaccg gtgcgggaca agcgcctact
     4981 tctcatacgg tcgcattgtc ctggaaccca acctcatccg gcggagttgc gggctataac
     5041 gtatttcggg cgagcacctc cggcggttat ggaactaacc ctctgaatcc cgtgccagtt
     5101 tccgtacctg catatgagga ttcgagtgtg acgggggggc aaacttactt ctatattgtg
     5161 acgacggtgg gtgccggcgt cagcagcgcc gcctcgaacg aagtggcggc aagcattcct
     5221 gcacaatgat cagttggcaa agacgagaca aatattcgca agcaacgtga ggcttccgtt
     5281 ggaagcgcgg actgacctct ggccgccagc cattcacgag cgcaccctct aaattcgaag
     5341 tgttccgtag gaatagtatc gccgcggaca ggggtaaaca cctgtcccaa gtgtgtacct
     5401 gcgtccagta gtctcccgtg ggccgcccgt ccacaatccg tacttcattt cgagaggtga
     5461 gaaatttcca cggccttcac gaagtatcca cggcggattc ctgtacgaac agcaattcgg
     5521 agccgcattt ttgcggcagc gatcgcactc atagctgttg ccggttgcgg agttggacct
     5581 agcctcgtac cgagttcgac gaaagtcgat ttcggcaacg tcgcggtcgg aggctcaacc
     5641 tcgcaactca taactctgac caacatggga aaaatgaacg tcaacattgc tcgggtttcc
     5701 gccacgggaa aaggattcag cgtgagcgga agatcgaacg tggttcttga acccaatcaa
     5761 gctgtcacgg tgtcagtagg tttcaatccc gcggcgaaag gcaaagcgca cggcgcccta
     5821 tccgtatcca gcgacgcatc gggcggcagg cttctgcgaa tcgccctatc gggcagcggc
     5881 gacgcaacat cgggcaatca cacggtggca ctcagctggc aagcgagttc ctcgccgaca
     5941 atcgggtact ttgtctaccg cagcagtaac ggaagtgggg tttcgaagct aaactcgacg
     6001 ttaaatgaat ccacgagttt caccgacagc acggtcgcga gtggagaaac ctaccaatat
     6061 gcggtgacgt cggtgagctc tagcaacgtc gaaagtgccg catccagccc aatcacagtt
     6121 acaattccta gtccctaagg accagacggc gcggatggtc tatccggtcc caaggtcagc
     6181 aatgaacgat cttttcgcgc ccagccttcg cctgccgcgt ggcattccga gtatcgatga
     6241 ccagggcgga ctcccgcacg atcgcatcat aatcgtactt tgagtggtcc gtggcgataa
     6301 ggacgcaatc atagctggcg agacgctcgg gggtcagttc gactgagcgt ttattcgaaa
     6361 aatcatagtg gcgcatcttg aagagcgccg ggaagtacgg atcattgtaa tcgacgaggg
     6421 cgccgcgctg ggtgagcagc tccagcaact taagagacgg cgactcccgc agatcgtcca
     6481 catccttctt gtaggccaca cccaacaaca aaattcgcga acctttgatg cttttttcgc
     6541 gctcattcag cgcttccgcg agcgcattta caacgtggta gggcatttca gtattgattt
     6601 caccggcgag ttcaatgaat cgcgtggcga aatcatattc gcgcgctttc cacgagaggt
     6661 aatacggatc aaccggaata cagtggccgc ccagacccgg tccaggataa aagggcataa
     6721 aaccgaacgg cttcgttgcc gcactattaa tcacctccca gatgtcgaga cccatgcgaa
     6781 gactgagctg ctttaattcg ttgaccagcg caatgttgac gcagcgaaat atattttcca
     6841 agagcttgac catctcagct gcccgcgtcg aactgacagg cacgactttg gagacgatct
     6901 gggcgtaaag agcagttgca gcctgtccac tagcaggatt gatacctcca actactttgg
     6961 gtatttgggc caaaccataa ttcttattgc ccggatcttc gcgctccggt gaaaaagcga
     7021 gatagaaatc agtcgccacc tcctttccac tcaatccgcc gtcgattggg cagcgcaagc
     7081 cgccggcggc gagtatcggc aaaacgagct cttccgtggt tccaggatat gtggtgctct
     7141 cgagcac
//
LOCUS       DATNDT010000007         2263 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4289996, whole genome shotgun sequence.
ACCESSION   DATNDT010000007 DATNDT010000000
VERSION     DATNDT010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4289996"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..754)
                     /locus_tag="VN788_00335"
     CDS             complement(107..754)
                     /locus_tag="VN788_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HXU19008.1"
                     /translation="MFADFIIPAIPTGTQLTALLVVICFSAGLNVYATVAMLGLLARG
                     HFLILPDSLHALVNWYVIGICAALFAVEFIGDKIPVFDLFWNAMHTFVRVPAAALLTY
                     AATAQLPEWERLAATFVGASIALAAHAGKVAARTAVTGSPEPFSNMALSMGEDLLVVF
                     LTWFATRHVYAAAAIALAGLAIIVVAIRAVIRAMMYLFRDTEHTVARSPKSAAMD"
     gene            complement(1068..>2263)
                     /locus_tag="VN788_00340"
     CDS             complement(1068..>2263)
                     /locus_tag="VN788_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HXU19009.1"
                     /translation="VGYGCTTCIGNSGPLPEHIAKAIKENNLVAVSVLSGNRNFEGRI
                     NPHCRANYLASPPLVVAYALAGRMDFDIVNEPIGNDKSGKPVYLREIWPTPQEVESTV
                     RSAVSSEMFRKEYADVFTGDAHWQALPVPEGDLYAWDGKSTYIKNPPYFEGMQVKPAA
                     IADLRGLRALAVLGDSITTDHISPAGSIQADGPAGKYLIGHGVQPKDFNSYGARRGNH
                     EVMMRGTFANIRLRNLLAPGTEGGWTVHQPGDEQMAIYDAAMKYREEGVPLVIIAGKE
                     YGSGSSRDWAAKGTLLLGVRAVLAESFERIHRSNLVGMGVLPLEFSPGEDFKSLGLSG
                     LEIFDFEGLGQNFEPKKKVKVRARDAQGKEKQFTAVARVDTPFEVAYYQHGGILQYVL
                     RQMLQ"
BASE COUNT          527 a          672 c          616 g          448 t
ORIGIN      
        1 tgggcggctt cttcgggaaa aagaaaaagt agcaaattgc gtttgcgtcg ctcgcttaaa
       61 cgggatttgc gtctcgccct ggacgagttg tgaggccgtg gtttcgtcaa tccatcgcag
      121 cagacttcgg tgaacgagca actgtgtgtt cggtgtcgcg aaataggtac atcattgcac
      181 gtataaccgc gcgaatggcc accacgatga tcgcgagtcc cgccagggca atcgccgcgg
      241 ccgcatacac gtggcgcgtc gcgaaccacg tgagaaatac aaccaacaga tcctcgccca
      301 tactgagcgc catgttggag aaaggttcgg gcgaccccgt caccgccgtt cgcgccgcca
      361 cctttcctgc atgcgccgcc agcgcgatcg aagctcccac aaatgttgcg gcgagccgct
      421 cccattccgg caattgcgcc gtggccgcat aggtgagcag cgctgcggcc ggcacgcgaa
      481 cgaacgtgtg catcgcgttc caaaacaaat caaaaaccgg aattttgtcg ccgataaatt
      541 cgaccgcgaa gagcgcggcg caaatgccaa tcacgtacca gttcaccaac gcatgaagcg
      601 aatccggaag tatcaggaaa tggccgcgag ccagcaaacc gagcatcgcc accgtggcgt
      661 acacattcag ccccgccgaa aagcaaatca caacgagcag cgccgtcagc tgcgtgccag
      721 tcggtatcgc cggaatgatg aaatcagcga acatagcgtg acgctaacga tacgacgccg
      781 cgcgtttccg cgctactgcg aaaggaagca acaaatgaca gatgcgcaag atcggaaacc
      841 aaaaccgggt gacatggtgg tgctcacaga agtcccgaag gggctttgta aacgatctac
      901 cgatagaaga tcaaaaagcg atttctgaga tcgtcgggaa gccaatcctg ctaaccgaat
      961 acgacaacca cggacgagcc gaactcaagt tcaccgatag tgaaggagtg attcaattca
     1021 ttttatctac gtcaacccaa ccgtcatcag acctatcgaa tagaaagcta ctgaagcatt
     1081 tggcgcaaaa cgtattgaag gattccgccg tgctggtagt aagcgacctc gaacggcgta
     1141 tcgacgcggg cgacggcggt aaactgtttt tccttgcctt gagcgtcgcg cgcgcgcact
     1201 ttgacctttt ttttcggctc gaaattctgg cccaagccct cgaaatcgaa aatttcgagg
     1261 ccgctaagcc cgagcgactt gaaatcttca cccggcgaga attccagtgg cagcactccc
     1321 atgccgacca gattgctgcg atggatgcgc tcgaaacttt ccgcaagcac cgcgcgaacc
     1381 ccgagaagca gagttccttt cgccgcccaa tcgcgcgacg agcctgagcc atattctttc
     1441 cccgcgataa ttactagcgg cacgccctct tcgcgatact tcatcgccgc gtcgtagatg
     1501 gccatctgct cgtcgcccgg ctgatggaca gtccagccgc cctccgttcc tggagcgagc
     1561 aaatttcgaa ggcggatatt ggcgaaagtt ccacgcatca tcacttcgtg attgccgcgc
     1621 cgcgccccgt aagaattgaa atccttcggc tgcacgccat gcccgatcag atattttccc
     1681 gcgggaccgt cggcctggat cgagcccgcc ggtgaaatat gatcggtagt gatgctatcc
     1741 cccagaaccg ctagcgcccg cagcccgcgc aaatcggcga tcgcggcggg ttttacctgc
     1801 atgccttcga aatatggcgg attcttgatg tatgtggatt tgccatccca cgcgtagaga
     1861 tcgccttcgg gaacgggcaa ggcctgccag tgcgcgtctc ccgtgaaaac gtcggcatat
     1921 tctttccgga acatttcgct tgagaccgcg ctgcgcaccg tggactcgac ttcttgaggc
     1981 gtgggccaga tttcgcgcaa gtaaaccggc ttgccggatt tgtcgttgcc aatcggctcg
     2041 ttgacgatat cgaaatccat gcggcccgca agcgcgtagg ccaccaccag cggcggcgac
     2101 gccagataat tcgcccggca gtgcggattg atgcgtcctt cgaaattgcg attgccgctg
     2161 aggacagaca cggcgacaag attattttcc ttgatggctt tcgcgatatg ttccggtagc
     2221 ggaccgctgt tgccgatgca agtggtgcag ccatagccca cca
//
LOCUS       DATNDT010000008         2731 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4003709, whole genome shotgun sequence.
ACCESSION   DATNDT010000008 DATNDT010000000
VERSION     DATNDT010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4003709"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..638
                     /locus_tag="VN788_00345"
     CDS             <1..638
                     /locus_tag="VN788_00345"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HXU19010.1"
                     /translation="EKVRKSMPMKCAGKTYMGRLALRDSGWRMGRLTVLGVFCVLLAA
                     PNLQGQRPAPSEYEVKATYLYNFARFTEWPAKDASVKGDSFGVCVLGRDPFGSILDTI
                     LAGEAIGGKQMVHRRISAAQDAGNCRIVFISSSEKSRLKETLAAMERMNVLTVSDIPD
                     FSVRGGMIEFVVERDKIRFEVNIETAGQAGLTLSSELLKVAVSIRNSQPGE"
     gene            644..2137
                     /locus_tag="VN788_00350"
     CDS             644..2137
                     /locus_tag="VN788_00350"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU19011.1"
                     /translation="MWRSRSYSLARKLTRMNLLVSCAALLTACAAFVAYDLATFRESV
                     VHNLSVEAQIIGSNSVSALEFDDPHSAEKTLSALHASPNIISAGIYGPDGRVFAAYSR
                     DRDAKAAPAFVALGNQTEAHLFRANEVLLTRSIVFGGKSAGSVRIRSDLESLYTRLRR
                     FGLIVAVVLGASLISAFLASSIFGKSTAEPIVRLAEIARVVSRDKNYSLRASPANTRD
                     ELDTLIEAFNEMLVQIQERDAALKKARDGLEQQVEQRTAQLTAANQELAAFSYSVSHD
                     LRAPLRHIAGFSAILMEDHGAQLAPAAQSYLTRIQDATENMGELIDDLLNLARIGRQD
                     MRRDTTDLNALVEGTIKDLETEQAGRQIDWHVKSLPTLDCDPGLIKVVFANLLSNAVK
                     YTRGREPAVIEVGTGSRDGVSTIYVRDNGVGFDPKYADKLFGVFQRLHRKEDFEGTGI
                     GLATVQRIVRRHGGTIWAEAEFGKGAAFYFTMQPTGNGAHNAAAEAD"
     gene            complement(2364..>2731)
                     /locus_tag="VN788_00355"
     CDS             complement(2364..>2731)
                     /locus_tag="VN788_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HXU19012.1"
                     /translation="NFRTLPNDGSRELPRDILNSRQIILEKQSLNVFDNPKANALIER
                     LPAETEFIVFGVVTEYCVQFAAKGLLDRHRQVSIVKDAIEALSPEAARKSLVELQAQG
                     ARLITTDEALHLLLVSTPS"
BASE COUNT          610 a          664 c          893 g          564 t
ORIGIN      
        1 tggagaaggt aagaaaatcg atgccgatga aatgcgctgg aaaaacttat atgggcagat
       61 tggcgctgag agatagcggg tggcgcatgg gccgcctgac ggttctaggc gtcttctgcg
      121 tcctacttgc ggccccaaat ctgcagggcc agcggccggc accgagcgaa tatgaggtga
      181 aggctacgta cctctacaat tttgcgcgat tcacggaatg gcccgcgaaa gatgcatctg
      241 tgaagggcga ctctttcggc gtatgcgtgc tgggacgcga tccctttggc tcgatcctcg
      301 acacgatcct ggcgggcgag gccattggcg gtaaacagat ggtgcacaga cgaatctcgg
      361 ccgctcagga cgcggggaat tgccgcattg tcttcatcag ttcgtcggag aaaagccggt
      421 taaaggaaac actcgccgct atggaaagaa tgaacgtact aacggtgagc gacattccgg
      481 acttttcagt gcgcggagga atgatcgagt ttgtggtgga gagggacaag attcgtttcg
      541 aggtgaatat cgagacggca ggccaagcgg gattgacgct aagttccgag cttctgaagg
      601 tcgcggtcag catccgaaat tcccagccag gagaatgaca caggtgtgga gatcacgcag
      661 ctattcgctg gccaggaaac tcacccggat gaatctgctg gtaagctgcg cggctttgct
      721 aacagcgtgc gcggcttttg tggcatacga cctggcgacg tttcgcgaat cggtggtcca
      781 taacctttcg gtggaggcgc agatcatcgg gtcgaatagc gtctccgcgc tggaattcga
      841 cgatccgcac tccgcagaaa aaacattgtc cgcgctgcat gcctcgccca acattatatc
      901 ggcgggaatt tacgggccgg acggccgggt gttcgcagca tattcacgag accgggacgc
      961 caaagcggca ccggcattcg tggcgctcgg gaatcaaacg gaagcgcatt tgttcagagc
     1021 caacgaggtg ttgctgacgc gctcgatcgt attcggcggc aagagtgcgg ggagcgtgcg
     1081 tatccggtct gacttggaat cgctttatac gcgactgagg cggtttggat taattgtcgc
     1141 ggtggtgttg ggagcgtctc tcatttcagc ttttctcgca tcgtctattt ttgggaaatc
     1201 cacggcagaa ccgattgtgc gtttggcgga gattgcgcgg gtggtgtccc gggacaagaa
     1261 ctattctctt agagcctctc ccgcgaatac gcgcgacgaa ctcgacactc tgatcgaggc
     1321 tttcaatgaa atgttggtgc agatacaaga gcgagacgcg gcgctgaaaa aagcgcgcga
     1381 cggattggag cagcaggtgg agcagcgaac cgcgcagttg acggccgcga atcaagagct
     1441 ggcggcattt tcctactccg tatcgcacga cctgcgcgca ccgttgcggc acatcgccgg
     1501 gttctcggca atcctgatgg aggatcacgg cgcccagctg gctcccgcgg cacaaagcta
     1561 cctgacgcgg attcaggacg ctacggaaaa catgggtgag ctgattgacg acttgctaaa
     1621 tttggcccgg atcggccggc aggacatgcg ccgagatacc acggatctga acgcactggt
     1681 ggaagggact atcaaagacc tcgaaacgga gcaggcgggg cgccaaatcg attggcacgt
     1741 aaaaagtttg cccacgctgg actgcgatcc gggcttgata aaggttgtgt tcgccaactt
     1801 gctatcaaat gccgtgaagt acacgcgcgg cagagagccc gcggtgatcg aggtgggaac
     1861 ggggtcgcgg gatggagtca gcacgattta tgtgcgcgac aacggcgtgg gtttcgatcc
     1921 aaagtatgcc gacaaattat tcggcgtgtt ccaacggctg caccggaaag aggatttcga
     1981 agggaccggc atcgggctgg caaccgtcca acgcattgtg agaaggcatg ggggaacgat
     2041 ttgggccgaa gcagagtttg gaaaaggggc ggcgttttat ttcacgatgc agccgacggg
     2101 gaatggggct cacaatgcgg cggctgaagc cgactagttg aactttaatt tggatttgct
     2161 tgcgaaaaag gcgcgcacga cgagtaatgc gttcgtcgtg gctacaattg cactcgaaga
     2221 tgccgggctg agagcggctg cgctacaaat gcaagagcgg cctcagattc gaggtgaata
     2281 gcgggcgcca cggcaagcat gtttgccggg caaggcatgc cgtgccccta caagtgcaaa
     2341 ggcagaagac gcgttcgcgg cggctaggac ggtgtggaca cgagaagaag atgcagagct
     2401 tcatcggtgg tgattaggcg ggcgccttgg gcttgcaatt cgacgaggga tttgcgggcg
     2461 gcctcggggg agagggcttc gattgcgtcc tttacgattg agacttggcg gtggcggtct
     2521 agcagacctt tcgcggcgaa ttgcacgcag tattcggtga ctacgccgaa gacgatgaat
     2581 tcggtttcgg cgggcaggcg ctcaataagc gcattcgctt tcggattatc gaaaacgttg
     2641 aggctttgct tttcgaggat tatctgccgg gagttcagaa tgtccctggg caattcgcgg
     2701 gatccatcgt tggggagcgt gcgaaaattt t
//
LOCUS       DATNDT010000009        10043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2292212, whole genome shotgun sequence.
ACCESSION   DATNDT010000009 DATNDT010000000
VERSION     DATNDT010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10043
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2292212"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="VN788_00360"
     CDS             <1..689
                     /locus_tag="VN788_00360"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXU19013.1"
                     /translation="QEVEKAITSRTRLVVITNLHNPTGARATDAQLRTIGDIAEKRGA
                     YVLVDEVYLDACFDPNSRSAFHLGGNFIIASSLTKAFGLSGLRCGWVLAPPSLAERMW
                     RINDLYGVIPAHVAELLSVIALDHLEKIADYARTRLQANRPILQKFLDSRRDLAAIPA
                     SGTTAFPRLASGKVDALCHLLREKYETSVVPGRFFELPNHFRIGIGSKTELLQEGLRR
                     LGHALDELHP"
     gene            complement(686..1591)
                     /locus_tag="VN788_00365"
     CDS             complement(686..1591)
                     /locus_tag="VN788_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXU19014.1"
                     /translation="MDLASLQVFLTVARERSFSRAAERLYRTQPAVSIAVRKLEEWVG
                     QPLLVRGTGAGKLTEAGELLVEYAERMLNLRSEAQRSIEDLAGLRRGRLSLGVNESSI
                     HALLPVLARFRRKWPKIEVAIHRVYSRDIPREILNYRLDIGVASYRASDDALEAVEFL
                     RDDLAFVVPPKHVFARKRAVSIRQLGEENFVAHIVASPYRQQVIQLFAKHRVPLRIGV
                     ELPTIESIKRFVRMGMGVAIVPRMCVRMEVEDRELIEVRIREMHLRRELYAIFRRDRE
                     LSHPAQAFLQLLRPKSSVQRTAAKS"
     gene            1708..2925
                     /locus_tag="VN788_00370"
     CDS             1708..2925
                     /locus_tag="VN788_00370"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: protein motif:HMM:NF002086.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXU19015.1"
                     /translation="MDTVRIFDTTLRDGEQSPGFSMTGPEKLLLARRLEALGVDVVEA
                     GFPVASAGELESVRAVAAAIRGTTVAALARARREDVEAALAALEPAARPRLHVFLATS
                     ELHLSAKLRITRHEALDAISTWVSYGKKRCPEVEFSAEDASRSDIRFLCEAMETAAEA
                     GATILNLPDTVGFSTPEEYGAMFHRVRSHLGRADEIIFSAHCHNDLGLAVANSLAAVR
                     AGARQVECTINGIGERAGNASLEELAVALAVRQSFFGVRTNLRLEEIYGASRALSDIT
                     GVSVPPNKAVVGANAFAHEAGIHQDGILKNPLTYEIIKPEAVGVPARRLVVGKHSGRN
                     ALRTRLEELGYRVNDRELAQCYRAAMDLADAEKEVRDRDLLAFIHMARRKNRHESPHA
                     DMAVNVPEPSTAQ"
     gene            2922..4037
                     /gene="leuB"
                     /locus_tag="VN788_00375"
     CDS             2922..4037
                     /gene="leuB"
                     /locus_tag="VN788_00375"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013085139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HXU19016.1"
                     /translation="MTGLRKTIAVVAGDGIGPEVIRAAISVLQECARAFGHQFQFVDL
                     PFGGDAIDRCGHPLPAETLDACRKADAVLLGAVGGAKWDSQPLDRRPEAGLLGLRKEL
                     GLYINVRPIKLLEPLRDICPIRLDEDESIDMEIVRELAGGIYFGPRKITGSNGDETAS
                     DTEIYSSKEIERVALYAFRRAVARGKRLASIDKANVLASSRLWRATVERISRDFPEVH
                     LESLYVDNAALQIVLRPAQFDVMLTSNMFGDILSDEAAALVGSIGLIPSMSLGNGSAL
                     YEPIHGSAPQLAGKDSANPIGAILCAAMLLRESFGLNREAAAIEFSVETVLNAGLRTP
                     DIARTGSRVVTCSAVASEIRAVLAESAVETENYGWGV"
     gene            complement(4061..4723)
                     /locus_tag="VN788_00380"
     CDS             complement(4061..4723)
                     /locus_tag="VN788_00380"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXU19017.1"
                     /translation="MFCSKCGSQVADNAGFCPTCGNPTGTQGTAPRGIPVAPGAFPPQ
                     AITPPSSYGSSAGQAAAYAAPVAQPIRWAGFWLRFVAWFIDSIILQIASTFIVLPFAV
                     STGIRDVILNHPPQSPAEIIALFGSLSKLIIISACIKWLYYALLESSAWQATIGKKTL
                     GLEVTDLEGHRISFARATGRYFAKIISILTFLIGYIMAGFTEKKQALHDMIAGCLVIR
                     KI"
     gene            complement(4810..5730)
                     /gene="bla"
                     /locus_tag="VN788_00385"
     CDS             complement(4810..5730)
                     /gene="bla"
                     /locus_tag="VN788_00385"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HXU19018.1"
                     /translation="MKMGSGEKRLSFVLFFLSCGALFCRPARSNPRPQADATPRSWNQ
                     PVEPFRIIGNIYYVGASGVTSFLIATPAGDILLDGGFVETASQIETNIKKLGFRLPDV
                     KILLSSHAHYDHAGGLTELKRATGATFYASTGDAPVLEGGGEGDSWFVHAVTFPPVKP
                     DRLLHDGSGIMFGGMALTARVTAGHTKGCTTWTTMVNEGGKSYHVVFVCSTSVLPGIK
                     LLGNQSYPKIASDYENTFRVLRSLPCDVFLASHGSFFDLKDKRNELLRRRKPNPFIDP
                     EGYRAYVDRSEAVFRAELRRQQGSAKSAAR"
     gene            complement(5744..6676)
                     /locus_tag="VN788_00390"
     CDS             complement(5744..6676)
                     /locus_tag="VN788_00390"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19019.1"
                     /translation="MSSSENGQLKTVLVTGGTDGLGRATAILLAQEGYRVFAAGRDAQ
                     KRAALEQFAEEHELRLETLALDVTDDVSAARAVGEIERRTGGVDVLVNNAGIAIVAVM
                     EEITLGDLRKQFETNMIAPVRMAQLVLPGMRERRRGRIINMSSIAGKISNPLMGPYSG
                     SKHALEAVSDAMRMELAHFGIQVVLIEPGFIPTNINQASAGLSAMYVQNAAASAYART
                     YSGFLEMWKKVVNRSRSKPEDCARVILQAIRADSPKARYLVTREAKISAAMKWLLSDR
                     RMDQITLRMLGMHHKASAMPQNIQAEIEEVVRTR"
     gene            complement(6738..6956)
                     /locus_tag="VN788_00395"
     CDS             complement(6738..6956)
                     /locus_tag="VN788_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19020.1"
                     /translation="MGTFERKDVSGTPPGRREEPPPSPRVLKLALIAAAIILLIGVLL
                     YLKARHAPPALHDELLPGARMANGVHGG"
     gene            7165..7299
                     /locus_tag="VN788_00400"
     CDS             7165..7299
                     /locus_tag="VN788_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19021.1"
                     /translation="MIKKVKGGYKVLSESGKNLGGPYKSKSQAEKRLRQVEFFKHRKG
                     "
     gene            complement(7322..7672)
                     /locus_tag="VN788_00405"
     CDS             complement(7322..7672)
                     /locus_tag="VN788_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19022.1"
                     /translation="MRTRPTRELNVPVSFRIPLQQLHEIEEAVQKEARRHRSDLLEFV
                     WNWAWSEYKKAGSLQALLAGTRTRHYSRRVSEELQDDLFTALETIIDRAPSTVIDDVA
                     RVLTQRAGKYGAEK"
     gene            7971..8234
                     /locus_tag="VN788_00410"
     CDS             7971..8234
                     /locus_tag="VN788_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19023.1"
                     /translation="MNDAFFPSNGLTVTVGQLIRQNGIQKIWESILLRCEEESDGTLA
                     IRVLISNPDWEQLLQIACIRSRPHDPESLTPLACNLDHEDLPA"
     gene            8835..>10043
                     /locus_tag="VN788_00415"
     rRNA            8835..>10043
                     /locus_tag="VN788_00415"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         2200 a         3025 c         2717 g         2101 t
ORIGIN      
        1 cgcaggaagt ggaaaaagca atcacctccc gcacgcgctt agttgtcatc acgaatctgc
       61 acaatcccac gggcgcacgc gccacagatg cccagctccg caccatcggg gacatcgccg
      121 aaaagcgtgg cgcctacgtt ctcgttgatg aagtctatct cgacgcctgt ttcgatccca
      181 actcgcgctc cgcattccat ctcggcggca atttcatcat cgccagcagc ctcacgaaag
      241 ctttcggcct ctccggcctt cgctgcggat gggttctcgc tccgccgtct ctcgccgagc
      301 gcatgtggcg tatcaatgac ctttatggcg tcatccccgc acacgtagcc gaattgctga
      361 gtgtaatcgc tctcgaccac cttgagaaaa ttgcagacta cgcgcgcacc cgtttgcagg
      421 cgaaccgccc catccttcaa aagttcctcg attcgcgccg tgatctcgcc gccattccgg
      481 ccagcggaac caccgccttt ccgcggctcg cgtcaggaaa agtggacgct ctttgtcatt
      541 tgctccgcga aaaatacgaa acgagcgttg tgccggggcg ttttttcgaa ctgccgaacc
      601 acttccgcat cggtatcggc agcaaaactg aacttttgca ggaaggtctt cgccggctcg
      661 gccatgccct tgatgaactt cacccctaac ttttcgctgc cgttcgctgg acggagcttt
      721 tagggcgcag cagttgtaga aacgcttgcg ccggatgaga aagctcccgg tcgcgccgga
      781 aaatcgcgta aagctcgcga cgcaagtgca tttcgcgaat gcgcacttca atcagctcac
      841 ggtcctcaac ctccattcgc acgcacatcc gcggaacaat tgccaccccc atgcccatgc
      901 gcacaaagcg cttgatactt tcaatcgtcg gcagttccac tccgatgcgc agcggcacgc
      961 gatgttttgc gaatagctga atcacttgct gccggtaagg ggacgcgaca atgtgcgcga
     1021 cgaaattctc ttcgcccagt tggcgaatgg aaactgcgcg ctttcgcgca aacacatgtt
     1081 tcggcggcac cacaaacgcg aggtcgtcgc gcaaaaactc caccgcttcg agcgcgtcat
     1141 ccgaagctcg gtagctggcc accccgatgt ccaggcggta attcaatatc tcccgcggaa
     1201 tatcgcgcga atacacgcga tggattgcca cttcgatctt cggccatttc cggcgaaagc
     1261 gcgccagcac cggaagcagc gcgtgaatcg aactttcgtt cacgccgagc gaaagcctcc
     1321 cgcgtcgcag tccggcaaga tcttcaatgc ttcgctgcgc ttcggaacgc aaattcaaca
     1381 tgcgctccgc gtactccacg agcagttcgc ctgcttccgt gagctttcct gcgcccgtcc
     1441 cgcgcaccag cagcggctgc ccaacccact cttccagttt ccgcacagca atgctcaccg
     1501 ccggctgcgt ccggtacaga cgttccgccg cgcgggaaaa gctgcgctcg cgcgccaccg
     1561 tcagaaacac ctgcaaagaa gcaagatcca tagggcctca gcagcaaagt gagtataagg
     1621 catatttatg ccaagtagaa tgattataaa ttggacgaat gttgccttgg cgcgttttac
     1681 tgaaagcgtt tttaaagggg cattctcatg gacacagtcc gaattttcga taccacactc
     1741 cgtgatggcg aacaatctcc gggattctcc atgaccggtc cggaaaagct tctcctcgcc
     1801 cgcagactcg aagcactggg cgtggatgtg gtcgaagccg ggtttccggt agcgtcggcg
     1861 ggagaactcg aatccgtcag agcggtcgcc gcagccatcc gcggaacgac agtcgctgca
     1921 ctggctcgcg cgcgacgcga agacgttgaa gcggcccttg ccgcgctcga accagccgcc
     1981 aggccgcgcc tccacgtttt tctcgccacc tctgagcttc acttgagcgc caagctgcga
     2041 atcacgcgcc acgaagcact cgacgccatt tccacatggg tcagctatgg aaaaaaacgc
     2101 tgccctgaag tggaattctc cgccgaggat gccagccgca gcgacattcg cttcctctgc
     2161 gaagctatgg aaaccgcggc ggaagccggc gcaacaattt tgaatctgcc cgataccgtc
     2221 ggcttcagca cacccgaaga atatggcgcc atgttccacc gcgtgcgcag ccatctggga
     2281 cgcgccgacg aaatcatttt cagcgcgcac tgccacaatg atctcggtct cgccgtcgcc
     2341 aattctcttg ccgccgttcg cgccggagcg cgccaggtcg aatgcacgat caacggcatt
     2401 ggcgaacgcg ccggcaacgc ttcgctggag gaactggccg ttgcgctggc tgtgcgccag
     2461 tccttcttcg gcgtgcgcac caacctccgc ctggaagaaa tttacggcgc cagccgcgcg
     2521 ctctccgata tcaccggcgt ttccgttccg ccaaacaaag cagtcgtcgg tgcgaacgct
     2581 ttcgcccatg aagccggcat ccatcaggac ggcattctga aaaatccgct cacttacgaa
     2641 atcatcaagc cggaagcggt cggcgttccg gcgcgccgcc tcgtcgtcgg aaagcattcc
     2701 ggccgcaacg ctctgcgcac cagactcgaa gagcttggct atcgcgtgaa tgacagagag
     2761 ctcgcccaat gttatcgtgc cgctatggat ttggccgacg cggaaaaaga agtaagagat
     2821 cgcgacctgc tggcctttat ccacatggcg cgccgcaaaa atcggcatga aagcccgcac
     2881 gccgatatgg ccgttaatgt ccccgagccg agtacagcgc aatgactggt cttcgcaaga
     2941 caatcgcggt tgtcgccggt gacggcatcg gtcctgaagt gattcgcgcc gcaatttctg
     3001 ttttgcagga atgcgcccgc gccttcggcc atcagtttca gttcgtcgat ttgccttttg
     3061 gcggcgacgc cattgaccgt tgtggccatc cgctccccgc cgagactctc gacgcttgcc
     3121 gcaaagccga cgccgtgctc ctcggcgcag tcggcggcgc gaaatgggat tcgcagccgc
     3181 tcgatcgcag gcccgaagcg ggcttgctcg gcttgcgcaa agagctgggc ctgtacatca
     3241 acgtgcgccc tatcaaactc ctcgaaccgc tgcgcgacat ttgtcccatt cgcctcgatg
     3301 aagatgaatc catcgatatg gaaatcgtgc gcgaacttgc cggtggaatt tatttcggcc
     3361 cgcgcaaaat caccggctca aatggcgacg aaaccgcttc cgatacggaa atttattctt
     3421 ccaaggaaat cgagcgcgtc gcactgtacg cctttcgccg ggcggtcgcg cgcggcaagc
     3481 gtctcgcgag catagacaaa gccaatgttc tggcgagttc gcggctctgg cgcgctaccg
     3541 tcgagcgcat cagccgcgat ttccccgaag ttcatctgga atcgctctac gtggataatg
     3601 ccgcgctgca aatcgttctt cgccccgcgc aattcgatgt gatgcttacc tccaatatgt
     3661 tcggcgatat tctgagcgac gaagccgcgg ctctggttgg ctccatcggc ctgattcctt
     3721 cgatgagcct tggcaatggc tctgcgctct acgaacccat tcacggttct gcgccgcagc
     3781 tcgccggaaa ggattctgcg aatcctatcg gcgcaatcct gtgcgcggcc atgttgctgc
     3841 gcgaatcgtt tggcctgaac cgcgaagccg ctgcaattga gttttcggtt gaaacggtgc
     3901 tcaacgccgg cctgcgcaca ccagatatcg cccgcaccgg ctcgcgcgtc gtcacttgtt
     3961 cagcagtggc ttctgaaatt cgcgcggtcc tcgccgaatc tgcggtcgaa acggaaaact
     4021 atggctgggg tgtttgaact aaagcgcaag ttgaagaaat tcagatcttt cgaatcacca
     4081 ggcagcccgc gatcatatcg tggagcgcct gtttcttttc cgtgaagccc gccatgatgt
     4141 acccgatcag gaatgtcaat atcgaaatga ttttcgcgaa ataccggccg gtggcgcggg
     4201 caaacgaaat tcgatgcccc tccagatccg taacctcaag tcccaatgtc ttttttccaa
     4261 tcgtcgcctg ccacgccgag ctttcaagca gcgcgtagta cagccatttg atgcacgcgc
     4321 tgatgatgat tagcttcgac aagctgccaa acagggcaat gatctctgcc ggggactgtg
     4381 gaggatgatt cagaataacg tctctgattc cggtgcttac cgcaaagggc aacacaataa
     4441 acgtactcgc aatctgaaga atgatcgaat cgataaacca tgcaacaaaa cgcagccaaa
     4501 atccggccca gcgaatcggc tgcgcaaccg gcgcagcata agcggcagcc tggccggctg
     4561 agcttccata cgagctaggc ggcgttatcg cttgcggcgg aaaagctccc ggcgccacgg
     4621 gaatcccgcg aggagctgtg ccctgggttc cggtcggatt gccgcatgtt ggacagaagc
     4681 cagcgttatc ggcaacctgc gagccgcatt tcgagcagaa catcaatcct cctcaattgc
     4741 actcctgcct cagttacaca tgtgaatgtt gcgaggcatc gtacttgcgc gcttgcggca
     4801 ggtcaagcgt tagcgcgccg ccgattttgc ggagccttgt tggcggcgca attccgcgcg
     4861 aaatactgct tcgcttctgt ccacgtatgc gcggtaaccc tccgggtcga tgaacggatt
     4921 tggcttgcgc ctgcggagca gctcgttgcg tttatctttc aggtcaaaga aagagccgtg
     4981 cgaagcaaga aaaacatcac acggcagtga gcgtagaacg cgaaacgtgt tttcgtaatc
     5041 gctggcgatt tttgggtagc tctgattgcc gagaagtttg attcccggca gcacggacgt
     5101 gctgcaaaca aatacaacgt gatacgactt accgccctca ttgaccatcg tcgtccacgt
     5161 agtgcagcct tttgtatggc ccgcggttac acgcgcagtt aaggccattc ctccaaacat
     5221 tatgccgctg ccgtcgtgca gcaagcgatc cggcttcacg ggcgggaacg tcactgcatg
     5281 cacaaaccac gagtctccct ctcctccgcc ctccagcacc ggagcgtcgc ccgtgctcgc
     5341 gtagaacgtt gcgccggtcg cgcgctttag ttccgtcaag ccgcccgcat ggtcgtaatg
     5401 cgcatggctg ctcagtagaa ttttcacgtc aggcaaacga aatcctaatt tcttgatgtt
     5461 ggtttcgatc tgcgacgctg tttcgacgaa gcctccgtcg agaaggatgt ctcccgccgg
     5521 agttgcaatc aaaaatgatg tgacgccgct cgcgcctacg taatagatgt ttccaataat
     5581 tcgaaacggc tcaaccggct gattccagga tctcggggtc gcatccgctt gcggccgggg
     5641 atttgatcgc gctggccggc aaaatagcgc gccacaagat aagaaaaaga gcacgaagct
     5701 cagccgcttt tcgccgcttc ccattttcat ttggaatctc attctagcgc gtccgcacca
     5761 cttcttcgat ttccgcctgg atgttctgcg gcatggcgct cgccttgtga tgcattccca
     5821 gcattcgcaa ggtaatctga tccattctcc ggtccgagag cagccatttc atagccgcgg
     5881 aaattttcgc ctcccgcgtc accaggtagc gcgccttcgg cgagtccgcg cgaatcgcct
     5941 gcagaatcac ccgcgcgcaa tcttccggct tgcttcgcga acgattcaca acttttttcc
     6001 acatctccag aaacccgctg tacgtccgcg cgtacgcgct cgccgccgca ttctgcacat
     6061 acattgcgga aagcccggcg ctcgcctgat tgatgttcgt gggaatgaac cccggctcga
     6121 tgagcaccac ttgaatcccg aagtgcgcca gttccatacg catggcatcc gaaacagcct
     6181 caagcgcgtg cttgctacca ctgtatggcc ccatcagagg attcgaaatc ttcccagcaa
     6241 tcgaactcat gttgataatt cgcccgcgcc gccgctcgcg cattcccgga agcaccagtt
     6301 gcgccatgcg caccggcgca atcatattgg tctcgaactg cttccgcaaa tcgccgagcg
     6361 taatctcttc catcaccgcg acgatggcga tgcccgcgtt gttcaccagc acgtccacgc
     6421 cgccggtccg ccgctcgatt tcgcccaccg cgcgcgccgc agatacgtcg tccgtcacgt
     6481 ccagcgcaag cgtttcgagc cgcagttcat gctcttccgc gaactgttcg agcgccgcgc
     6541 gcttctgcgc gtcccggccg gcggcgaata ctcggtaacc ttcctgcgcc agcagaatgg
     6601 ccgtcgctcg tccaagtcca tcggttccac cggtgacaag gaccgtcttt aactggccat
     6661 tttcggagga actcatcgtc acctcctggt gattttcgtc acaggaatgc ccaaaaagct
     6721 cgcgtgaacc ttaatcgtca gccgccgtga acaccgttcg ccatccgcgc acccggcagc
     6781 agttcatcat gcaacgccgg aggagcatgg cgcgccttca gataaagcaa aacaccaatg
     6841 agcaggataa tcgccgcagc aatcagcgcc agcttcaaaa cgcgcggaga aggcggaggc
     6901 tcttcgcgcc gccctggcgg agtgcccgaa acgtctttcc tttcgaacgt gcccataact
     6961 ctaactgtgc ctcagttgct ctgccaaagg cccacaggaa ttccttgcgc accggcttac
     7021 tccagccatg ctaggccgcg cacctcatca agtcaacgca cgctcgccga gtcgcgtgat
     7081 tgctctactg cttttggccg atggacgcac acgcctgctc gcgtgatgat ggaaaccagt
     7141 ctggacacga acgaaggagg agaaatgatc aagaaagtga aaggcggcta caaagtgctt
     7201 tccgaaagcg gcaagaatct cggagggccg tacaagtcga aatcacaagc cgaaaagcgt
     7261 ttgcgtcagg tcgaattttt caagcatcgc aagggataac tcgcccgcga aaggctggcg
     7321 ctcacttttc ggcaccgtat ttcccggctc gttgcgtcag cacgcgcgcg acatcgtcaa
     7381 tcaccgtgct cggcgcccga tcgataatgg tttccagcgc cgtgaataga tcatcctgaa
     7441 gctcctcaga cacccttcgc gaatagtgcc gcgtgcgcgt cccagccagc aacgcctgca
     7501 gggaaccggc ctttttgtac tcgctccacg cccagttcca cacgaattcg agcaggtccg
     7561 aacggtggcg gcgcgcctcc ttctgcaccg cctcctcgat ttcgtgcagt tgttgcagcg
     7621 ggattcgaaa gcttaccggc acgttcagtt cgcgcgtcgg ccttgttcgc attccgtcat
     7681 ggtgcccgtg cggatttctg ttgacaagca atatctgccg attcatcgta tgcagtgtat
     7741 gcatacaagt catacatacc tagttgagag aacaacttac aacggggtgc ttgtctatta
     7801 tgggtacgtt caagcgcgaa gtttcttctc attccgagtc tctgaagcac gaccagcgac
     7861 tcagccctaa atccatgccc acgcgcgatg ataaggtgcg cgtcatgccc caagaacggc
     7921 gcccgccacg tctcgctgcc cccttttttg ttactcgttg cgaagcccgc ttgaatgatg
     7981 ctttcttccc atccaatggg cttactgtca cggtgggtca gttaattcga cagaatggca
     8041 ttcagaaaat ctgggaatca atcctgctgc gctgtgaaga agaaagcgac ggcaccctcg
     8101 ccatccgcgt cctcatctct aaccccgatt gggagcagtt gctgcaaatc gcgtgtatcc
     8161 gttcgcgtcc ccatgacccc gagtccctca caccgctggc ctgcaatttg gaccacgagg
     8221 atcttccggc ctaatatcac ggcgtttcct tcaggggttc gatttccaca aaggctttga
     8281 aattcacagg gcaatccagc aaacctcgta aaaaagtttt gcatctgact tgacgtatcg
     8341 ggaaaagtgg gtatactttc ctgatgtcgg aagaccgggc acgcccggag gacccggcgg
     8401 gccggttatc tcccctgaat taacgaggtg ggattggtta cgaataccgg atagaaaaca
     8461 gtcgagcttc gagcaagccc ttccaaagaa ggctgtggaa ggaatcgccc ggcgcttgac
     8521 gccgggtttt ttattttccg tcacgcctgt tttgcgagcg tgcggctccg ccgggagcgg
     8581 agagtcctga agcagcagct tttcagggca tagttcattg acaactgaag gaagtggcat
     8641 tgcgcagcac gatttctcaa gttcgtgcgg cgcaatatcc gcaagttttg ctcaagtagc
     8701 gcaatgtgga aaggcgaact ctctgcatga agcgcaagct gacttgaggt atgccgatga
     8761 agggcttgca agactctttg tcgcggcatt ctcaggattc cggcgcaagc cgggacgtgt
     8821 taggtgagtt ttatggtcaa gctactaagg gcgtacggtg gatgccttgg cgccaacaga
     8881 cgacgaagga cgtggctagc tgcgataagc ctcggggagc cgcaagcagg ctttgatccg
     8941 gggatttccg aatggggaaa cccgtaccga tgaactcggt atacagcccg ctgaatccat
     9001 aggcgggtat gagtcaaccc agggaagtga accatctcag tacctggagg aagagaaatc
     9061 aaacgagatt ccgagagtag cggcgagcga aatcggaaca gcccaaactt cggtgcttgc
     9121 accggagggt tgtaggaccg cataagtaga gttatcaatc cggaggctag gtgaacctgc
     9181 tggaaagcgg ggccaaagaa ggtgatagcc ctgtaaccga aagcttaccg gactcgaagc
     9241 ggtatcctga gtactgcgag acacgtgcaa tctcgcagga atctggggag accactctcc
     9301 aaggctaaat actcgttggc gaccgatagt gaaccagtac cgtgagggaa aggtgaaaag
     9361 cacccctgcg aggggagtga aatagaacct gaaaccgtat gcttacaagc agtcggaggg
     9421 ctatgacacg acttcggtcg tgtaaatgcc tgacggcgtg cctcttgctt aatgagccgg
     9481 ctagttattc tcccaggcaa ggttaagcgt aaagcgagcc gaagcgaaag cgagtccgaa
     9541 tagggcgaca agtctgtggg aatagacgcg aagcgggatg atctaccctt ggccaggttg
     9601 aaggctgctt aaccgcagct ggaggaccga accgttgtga gttgaaaatc gctcggatga
     9661 gctgagggta ggggtgaaag gctaatcaaa ttccgtgata gctcgttctc cccgaaatgc
     9721 ctttaggggc agcctcgtgt gatctgccac ggtggtagag cactgaatgg actagggtcc
     9781 catccaggat accgaatcca atcaaactcc gaatgccgtg aacagtaact cgggagtgag
     9841 actacggggg ataagctccg tggtcgagag ggaaagaacc cagatcgcca tctaaggtcc
     9901 ccaagtacaa gctaagtggg aaaggacgtg tgggcgcaca gacagccagg aggttggctt
     9961 agaagcagcc atcctttaaa gaaagcgtaa cagctcactg gtcaagtgct cacgcaccga
    10021 caatccaacg gggctcaagc ttg
//
LOCUS       DATNDT010000010        13185 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2866227, whole genome shotgun sequence.
ACCESSION   DATNDT010000010 DATNDT010000000
VERSION     DATNDT010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13185)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13185)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13185
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2866227"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VN788_00420"
     CDS             complement(<1..686)
                     /locus_tag="VN788_00420"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HXU19024.1"
                     /translation="MSALHVLGMVFLVGVNAFFSATEFSLVAVRLSRIRQLVEKGDPR
                     AKIVEQLLGDLGRVVSGVQVGITIASLSLGYLGEVTLASLLRPMVEKMVRPWAAIAAH
                     GAALIVVFGLLTVLQVIFGELVPKSLSLARAERVALLIARPFNWFLNTLRWAIDFLDA
                     AAERIVRVLGVLAPHSHTLVRSAEELMVMIQQARDRGLIPAAEARFVQNAIDLGRVQA
                     HEVMVPRPDV"
     gene            986..1252
                     /locus_tag="VN788_00425"
     CDS             986..1252
                     /locus_tag="VN788_00425"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018414872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HXU19025.1"
                     /translation="MSSPANKPAEPQRKGDFEKSLVRLEEVVKRLESADLSLDEAMKL
                     FEEGVKLSRECQKQLEEAEGRVEILLKKADGKIAAEPFEPEDES"
     gene            1315..2196
                     /locus_tag="VN788_00430"
     CDS             1315..2196
                     /locus_tag="VN788_00430"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HXU19026.1"
                     /translation="MKVPDFFETDHAIIEAGLNRFLPAESAAPSLIHRSMRYSVFAGG
                     KRIRPILCLEAARMFNENPEKAIQAGCALEFIHTYSLIHDDLPALDNDDLRRGKATNH
                     KVFGEAMAILAGDALLTLAFQTLASMPLEAERRLAVLELVATAAGTDRGMIGGQVADL
                     EAERKEVDSAGLEYIHRSKTAALIRASIVAGGIAGGADGADVRRLGAFGENIGWAFQV
                     VDDLLDVEESSAVLGKTAGKDEAQKKATYPAFYGLEKSRAIASDLEAKALAELSYYGE
                     RASRLRQLAQFLVARRS"
     gene            2233..2994
                     /locus_tag="VN788_00435"
     CDS             2233..2994
                     /locus_tag="VN788_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006982699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HXU19027.1"
                     /translation="MAGRNSSVKVTKHRLDLLLVERGIAPSRQRAQALLLAGQVRVNG
                     TRMDKPGTPVPVDARIETTGEQLRYASRGGLKLEGAIADFGVSPQGAVCLDVGSSNGG
                     FTDCLLQNGARRVYAVDVTIDQLDWKLRRDPRVTTIEKNARYLRVEDLAEQVKFVTVD
                     VSFISLSKILPAIVRVAASGAIFLNLIKPQFELDRRDIGKGGIVRDPELHKKAIDGVV
                     SAASSLGLEIGGVLPSRIAGTEGNQEFFLHARRGL"
     gene            3018..3878
                     /locus_tag="VN788_00440"
     CDS             3018..3878
                     /locus_tag="VN788_00440"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HXU19028.1"
                     /translation="MIRTAGIICKPIKEVVSSVVPPLVEWLRGHKIEVLIDQETQACV
                     GVVSHALSRDALGEKIEILIVLGGDGTLLSAARALGGHRVPILAVNLGGLGFLTSVTL
                     DELYPLLERVLAGKHRISERMMLDAEIVRDGQSAKRQCALNDAVANKGAVARMLDFDV
                     SVNGDAVGRYRADGIIVATPTGSTAYSLAAGGPIVDPDLDAFIITPICPHMLTNRPLV
                     IPGAARVELDFAAADDPVYITLDGQIGFELKANDRVAITKSSNRVALIRPPRKTYFEV
                     LRSKLRWGER"
     gene            complement(3895..5646)
                     /locus_tag="VN788_00445"
     CDS             complement(3895..5646)
                     /locus_tag="VN788_00445"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXU19029.1"
                     /translation="MDSTELNSVQEVPAETLAVLSEISHEINSSLNLDQVLASAAQQV
                     KRLMDYEIFAVLLPVENTNDLYVRFAIGHRPEVIEHWRIPMGDGIIGAAATTGQAIRV
                     GDVLKDPRYLPAVDAVRSELAVPLIVRGRVIGVVDIESRQPDYFTPAQQTILTLVASR
                     IGTAVENARLFENARKQAETLLLLNEIGRETSATLQVEEVLRRAAQLTKRLIDYQIFS
                     ILLYDEAAAAFRHRVTVKFGQQVQEKSAVPAHEGIVGAAAKLRRPVVVPDVSLDPRYR
                     MLNPETRSELAVPMIYKNRVVGVMDLESPQLNYFTPDHVQVLSILAAHLAVSIENARL
                     YEQLARDESRMERDLSAARRIQGALLPILPGPEFGLDIAARVVSSRELSGDLYDFIRY
                     GPQDLGIALGDVSGKGSAAALYGAVAVGTLRSLGSQKPQPASMLRALNGFLGERLIEG
                     RFMTICFATWNRKQRRLRVANAGQEQPLLCHAGRCEKIPLTGFPLGIFQEATYDQRSI
                     VLDHGDVVVFYSDGIGDAQNSSGEFFGYGRVAKILADHTSLPADGIADKVLEAADAFS
                     GGQHPADDRTLIVLKVR"
     gene            complement(5839..6369)
                     /gene="hpt"
                     /locus_tag="VN788_00450"
     CDS             complement(5839..6369)
                     /gene="hpt"
                     /locus_tag="VN788_00450"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004270773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HXU19030.1"
                     /translation="MSKVLILRSAIEKRVKAVARTITRDYKGQRVHLIGVLKGACFFL
                     ADLAREINLETSIDFISVASYGKGKDSSGQVRLLKDLDSSIAGLNVILVEDILDTGRT
                     LRYLLRILRQRKPKSLRVAALLDKPSRRVTPVKADYIGFTIPNEFVVGYGLDYAERYR
                     NLKDVCVLTSSSHSPQ"
     gene            6470..7129
                     /locus_tag="VN788_00455"
     CDS             6470..7129
                     /locus_tag="VN788_00455"
                     /inference="COORDINATES: protein motif:HMM:NF016825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HXU19031.1"
                     /translation="MMSSPAWESTLEQFRSAAASSEPIPAGVAVAATSASFALGLLSK
                     VLKVTGRKKGFSGDLAKVEKLAESARAESKRMMQLAEDDMAAFNDYMISARLPQGTDR
                     ERDERNRAVNASVRKAIETPVAAARAAASGISLCGDALDSVHAIVAADLGAAASLLAG
                     ALRVFLLCADSNIRQLAPDPPAYRDVLPGRAEWEARAFSQADFVLKKVSEVIDAVTKK
                     P"
     gene            complement(7139..9037)
                     /locus_tag="VN788_00460"
     CDS             complement(7139..9037)
                     /locus_tag="VN788_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0035999 - tetrahydrofolate interconversion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HXU19032.1"
                     /translation="MPHKLQNILARVPSDIDIAQAATPIPIDKIAAEVGILPDELDLY
                     GKSKAKVHLSVRDRLKNTPNGKYIVVTAITPTPLGEGKTTTTVGLSQAIGAHLGKKVF
                     TCIRQPSQGPTFGIKGGAAGGGYSQIIPMEEFNLHLTGDIHAITAANNLLAAAIDTRW
                     FHEKSQSDETLFERLCPPAKDGSRRFSPVMLRRLRKLGITKTNPNDLTPEERSRFARL
                     DIDVDSITWRRVTDTNDRMLRQITIGQGPEEKGVTRVTGFDISVASEIMAILALATDL
                     ADMRERLGRMVIGTNKAGEAITADDIGVGGALTVLMKDTIMPNLMQTLEGTPAFVHAG
                     PFGNIAHGNSSIVADQVALKLVGPDGYVLTESGFGADLGMEKFFNIKCRYSGLVPNCV
                     VMVATIRALKMHGGGPKVVAGQPLNPAYTEENLELLEKGCSNLIKMIDNARKYGIPVV
                     VGVNRFQYDTPAEVDLVRKIAMKAGAVDAVMSNHWAQGGAGAVELGKAVIAACEKPSK
                     FQFLYPLEMGIKQKIETIVREMYGGAGVEYSPEAEKKIALYDRLGFNKLPMCMAKTHL
                     SLSHDANLKGAPTGFTVPVRDVRASVGAGFLYPLLGTMSTMPGLSTRPGYYEIDIDLK
                     TGRIIGLS"
     gene            complement(9685..12531)
                     /locus_tag="VN788_00465"
     CDS             complement(9685..12531)
                     /locus_tag="VN788_00465"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HXU19033.1"
                     /translation="MAETRTALNTPMQTEKIARRASGLDFQRRFTDGKVSPFDRVEWE
                     RRTALIGNDKGQVIFRQENVEVPKSWSQTATNIVTSKYFHGKPNSPERENSVRQLISR
                     VADTIVRWGEAGGYFATAESKDAFHDELTHLLVEQKMAFNSPVWFNVGVQAKPQCSAC
                     FINSVQDNMESIMNLAKTEGMLFKWGSGTGTNFSSLRGSKESLSGGGIASGPVSFMKG
                     FDAFAGVIKSGGKTRRAAKMVILNVDHPDIVEFIESKTREERKAHVLIEQGYDSAIDG
                     DAYSSVFFQNANHSVRVTDDFMRAVEEDRDWWTRNVTDGEPVDQHRARDLMKMMAESA
                     WRCGDPGMQYDTTINRWHTSKATDRIYASNPCSEYMFLDDTACNLASLNLLKFLGTNG
                     QFDTEGFRHAVDITITAQEILVDNASYPTEKIARNSHDYRPLGIGYANLGALLMSMAL
                     PYDSDGGRDVCGVITALMTGESYAQSARIAERMGPFGGYPRNRESMLDVIRMHRDSLR
                     PIKEENVQPSLLHAAQESWDAALELGEKFGYKNSQVSVLAPTGTIGFMMDCDTTGIEP
                     DLALVKQKRLVGGGVIKIVNNTVPQALMNLGYPPEQVAQIVDYIDAQGKIEGAPGLKP
                     EHLPVFDCSLTPMGGGRSISWRGHLRMMAAAQPFLSGAISKTINMPEESTVEDIAEAY
                     MESWKFGLKAVAIYRDNSKRSQPLSAAGKKEEEKAAAPAKAAVMLAPATLQGAFQASP
                     QPAVPGPEQQELFVRARREKLPAERRSITHKFSVGGHEGYITVGMYDDGKPGEIFIKM
                     AKEGSTLSGIMDAFALSVSISLQYGVPLRALVDKFCNSRFEPSGYTGNPKIRYAKSIV
                     DYIGRWLGGKFISPDYLDRDDVAVPERGADAGSAAAVVAPGAFAIPGAIEETTTRPRA
                     AMEDAPYCSECGMLMTPNGSCYKCSNCGSTSGCS"
BASE COUNT         2779 a         3832 c         3770 g         2804 t
ORIGIN      
        1 tgcacatccg ggcggggaac cattacttca tgcgcctgca ctcgaccgag gtcaatcgcg
       61 ttttgaacaa atctcgcttc ggccgccgga ataaggccgc ggtcccgcgc ctgctgaatc
      121 atcaccatca attcttcggc ggacctgacc agggtgtggc tgtgaggagc aagtacacct
      181 agcacgcgga cgattctctc cgcagcggcg tcgaggaaat cgatggccca gcgaagggtg
      241 ttaaggaacc agttaaacgg acgcgctata agaagcgcga cgcgttcggc acgggccaag
      301 ctcaaacttt tggggacgag ctcaccgaat atcacctgca agaccgtgag caatccaaac
      361 acgacgatga gcgcggcacc gtgggccgcg atggcggccc atggtcgcac cattttttca
      421 accattggcc ggagaagcga cgccaacgtg acctctccca aatatccaag cgagaggctg
      481 gctatagtga tgccaacttg cacaccggaa acgacccggc caagatctcc cagaagttgt
      541 tcgacgatct tggcgcgagg atcgcctttt tccaccagct gccgaatgcg cgaaagcctg
      601 acggctacga gggagaattc cgtcgctgaa aaaaatgcgt ttacgccgac taaaaagacc
      661 atgccgagaa catgaagggc gctcatcgga cggggttatc taatcgggag agatttacgg
      721 gatgattcat agagttcggc caagcgattc agcgcaggcc tgggcaatgg cttgattccc
      781 cgtcagcccg taataacgga gaatcgaggt ggaatcctcc aagtaagaac ttgcaaatcc
      841 gagcggcata gaccgattgt ccgcttttcg ctcgcgtatt cgcaagcggg tgagtcgtgg
      901 gacagcaagc actcccttgt tgacgttctg tgccctaggc tagaatggcc ttcgtgcaga
      961 tcgggcgcaa aaaccgaccg gaacgatgag ttcacctgcc aacaaaccag cggagccgca
     1021 gcgaaagggt gattttgaaa agtcactcgt gcgcctggag gaagtggtta agaggctcga
     1081 gagcgccgat ctttcgctcg atgaggccat gaaattgttt gaagaaggcg ttaaactttc
     1141 ccgcgaatgc cagaagcagc ttgaggaagc ggagggtcgc gtggaaattc ttctaaagaa
     1201 ggcggacggg aagatagccg cggaaccctt cgagccggaa gatgaatcgt gacagcgttt
     1261 ctcccgagtt gtcgtttgcg cggcgccttt gaaaattacg cctgaatctt ccgcatgaaa
     1321 gtccccgatt tcttcgagac ggaccacgcc ataattgaag ctggtctcaa ccgttttctc
     1381 cccgccgaaa gtgctgcgcc atcattaatc catcgctcga tgcggtacag cgtgttcgcc
     1441 ggcggcaaaa ggattcggcc aattttgtgt ctcgaagctg cgcgaatgtt caacgaaaac
     1501 ccggagaagg ccatccaggc tggctgcgcg ctggaattta ttcacactta ttcgctgatc
     1561 cacgacgatc tgccggcgct ggacaatgat gatttgcgcc gcggcaaagc caccaatcac
     1621 aaggtgtttg gagaagccat ggcgatcctg gcgggcgatg cgctgcttac gcttgctttt
     1681 caaacactgg catccatgcc gctggaagcg gaaaggcgcc tggccgttct ggaattggtt
     1741 gcgacggccg caggcactga ccggggcatg atcggaggac aggtggccga cctggaagcc
     1801 gaacgcaaag aggtggatag tgcggggttg gagtacatcc accgatcgaa aacggccgca
     1861 ctgattcgcg cgtcgatcgt ggcgggcggc atcgccggcg gagccgatgg tgcggatgtc
     1921 agacggctgg gcgcattcgg cgagaacatt ggatgggcat tccaagtggt ggacgatttg
     1981 ctcgatgtgg aggaatcatc ggcggtcctg gggaaaaccg ctgggaagga tgaggcacaa
     2041 aaaaaagcga cgtatccggc gttttacggc ctggaaaaat cgcgcgcgat tgcatccgac
     2101 ttagaggcga aagcactggc ggaactctcc tattacggag aacgcgcctc gcggttgcgt
     2161 caacttgcgc aatttcttgt cgcgcgccgc tcgtagcttt actcactcat ggaaaccagg
     2221 aaatcagcca aagtggcagg gcgaaactca tccgtaaaag tgacgaagca ccgcctcgac
     2281 ctgctcctcg tcgagcgcgg catcgccccg tccaggcagc gggcacaggc gctgctgttg
     2341 gccggacaag tgcgcgtgaa cggcacccgg atggacaagc cgggcacgcc agtgccggtt
     2401 gatgcgcgaa tagaaacaac gggagaacag cttcgctacg ccagccgagg ggggctaaag
     2461 ctcgaaggcg cgatcgcgga tttcggcgtg agcccgcaag gtgcggtgtg cctggacgta
     2521 gggagctcga acggcggctt cacagattgc ctgctgcaaa acggggcacg cagagtttac
     2581 gcggtggatg tgaccatcga tcaattggac tggaagctgc ggcgcgaccc gcgagtgacc
     2641 acgattgaaa aaaatgcgcg ctatcttcgc gttgaggatc tggccgaaca ggtgaaattt
     2701 gttacggtcg atgtctcctt catttcgctg agcaaaattc ttccggctat tgtgcgcgta
     2761 gcggcaagcg gcgccatttt tctgaatcta attaaaccgc aatttgagtt ggatagacga
     2821 gacatcggca aaggcgggat cgttcgagat ccggagttgc acaagaaagc catcgacggc
     2881 gtggtgagcg cggcttccag tctgggatta gagatcggcg gcgtactccc cagccggatt
     2941 gcagggaccg aaggaaacca ggagtttttt ctccacgcga gacgcggact ctagaataga
     3001 atgcagctct ccgaccaatg attcgcaccg ccggcatcat ctgcaagccc atcaaagagg
     3061 tcgtttcgtc cgttgtaccc ccgctggtcg aatggctgcg cggtcataaa attgaagtcc
     3121 tcatcgatca agagacgcaa gcgtgcgtgg gggtcgtgag tcacgcgctt tctcgcgacg
     3181 cactcggcga aaagatcgaa attttgatcg tactgggcgg agacggcacg ctgctttcgg
     3241 cagccagggc gctcggcggc caccgggtac cgatcctggc ggttaacctg ggcggccttg
     3301 ggtttctcac cagcgtgacg cttgacgaac tctatccatt gctcgaacga gtgcttgcgg
     3361 gaaagcatcg catcagcgag cgcatgatgc tggatgctga gattgtgcgc gacgggcaat
     3421 ccgccaaacg gcaatgcgcg ctgaatgatg cggtggccaa taaaggcgcg gtggctcgga
     3481 tgctcgactt cgatgtatca gtgaacggcg acgccgtggg ccggtatcgc gctgatggaa
     3541 tcatcgtcgc aacgcctacc ggctcgactg cctattcgct ggcagccggt gggcccatcg
     3601 tggatcccga tctcgatgcc ttcatcatca cgccgatatg ccctcacatg ctgacaaatc
     3661 ggcctctggt gatcccgggc gcggctagag tggaactcga tttcgcggcc gcggacgacc
     3721 ctgtttacat taccctcgat gggcaaatag gatttgagtt gaaggccaac gatcgggtgg
     3781 cgattacaaa gtcgtccaac cgtgttgcgc taatacgccc gccaagaaaa acctacttcg
     3841 aagtcttgcg gagtaagctt cgctggggcg aacgctgaac actccgccat ttcatcagcg
     3901 cactttcaga acaatcaagg tgcggtcgtc agccggatgc tggcccccgg agaacgcatc
     3961 cgcggcctcg aggactttgt ccgcaattcc gtccgccggc aggctcgtgt ggtcggcaag
     4021 aatcttggcg acgcggccgt agccgaagaa ctctccggat gaattttggg catccccgat
     4081 tccatccgag taaaacacca cgacgtcgcc gtggtcgagg acaatggatc gctggtcgta
     4141 ggtcgcctct tgaaaaattc cgagagggaa gcccgtgagg gggatttttt cgcatcgccc
     4201 ggcgtggcaa agaagcggct gttcctgacc ggcatttgca acccgcagcc gacgctgctt
     4261 gcgattccat gtggcgaagc aaatggtcat gaagcgacct tcgatcaatc gttctcccaa
     4321 aaacccattg agggcgcgca gcatcgacgc gggctgaggt ttctgagagc ccagactgcg
     4381 caaagtgccg accgccacgg cgccatagag agccgccgcg ctccctttgc cgctgacatc
     4441 gcccagagcg atgccgagat cctggggacc gtagcgaata aaatcgtaga ggtccccgct
     4501 cagttctctc gaagacacaa ctcgtgcggc aatatccaaa ccgaactcgg gcccaggcag
     4561 aatggggagt agcgctcctt gaatgcggcg cgccgcgctg agatcgcgtt ccatgcggct
     4621 ttcgtcgcga gccaattgct cgtaaaggcg cgcgttttcg atcgagacgg cgagatgcgc
     4681 cgccaaaatc gaaagcactt gaacgtgatc cggcgtgaag taattcagct gcgggctttc
     4741 aagatccatc acacccacca ctcggttttt gtagatcatc ggtaccgcga gttccgatcg
     4801 cgtttccgga ttgagcatcc ggtaacgcgg gtcgagtgaa acgtcaggaa ccacaaccgg
     4861 acggcgcagc ttggcagcgg cgcccacgat tccttcgtgg gcgggaaccg cggatttttc
     4921 ctgtacctgc tgcccgaatt tcaccgtcac gcgatggcga aaggccgcgg ccgcctcgtc
     4981 ataaagcaat atgctgaaga tttgatagtc gatcaagcgt ttcgtcaatt gcgccgcgcg
     5041 ccgcaggact tcttccactt gcagcgttgc gctcgtttcc cttccaatct cattgagtag
     5101 gagcaacgtt tccgcctgct tccgcgcatt ctcgaaaagc cgcgcattct ccacggcggt
     5161 tccgatgcgg cttgcgacca gcgtcaggat cgtctgctgg gcaggagtaa aataatctgg
     5221 ctggcgactc tcaatatcca ccacgccaat cactcgtcct cggacgatca gcggcaccgc
     5281 caactccgac cgaaccgcat cgacggcagg cagatagcgc ggatctttca atacatcgcc
     5341 gacgcgaatg gcctggcccg tagtggcggc ggccccgatg attccatcgc ccatggggat
     5401 gcgccagtgt tcaatcacct cgggccggtg gccaatggca aaacggacgt acagatcgtt
     5461 tgtgttttcc acgggcagca gaacggcaaa aatctcatag tccatcagcc gcttcacctg
     5521 ctgagccgcg ctggcgagca cctggtccag atttagcgac gagttgattt cgtggctaat
     5581 ctccgagagg actgccaggg tctcggcagg gacttcctgc accgaattga gctctgtgga
     5641 atccatttga attgctgacg cgcacgcgcg cggtacctcg gcgacaatgc cgccagatta
     5701 gcaggcatct ccccagcact tcggcgcaaa cgcgatgggg ccattatatc aggcgcggaa
     5761 tcgaaccttg gctgcaccag aagccaagcg ctgggtcgaa ataggccgtt gagacgtccg
     5821 cgaaggctgg caaaggctct attgaggtga atgcgaagaa gacgtgagga cgcacacatc
     5881 tttgaggttg cgatagcgct ccgcgtaatc cagtccatag ccgaccacaa attcatttgg
     5941 aatggtaaag ccaatgtaat cggccttcac cggagtcacg cggcgtgagg gtttatcgag
     6001 tagcgccgcc acgcgcaacg acttcggctt gcgctgccgc aaaatgcgca gcaggtagcg
     6061 cagcgtccgt cctgtgtcga gaatgtcctc gaccagaatc acattcagtc ccgcaatact
     6121 gctatccaaa tctttcagta atcggacctg tcctgacgag tccttcccct tgccatagct
     6181 tgccaccgag atgaaatcaa tggacgtctc gagattgatt tcccgcgcga gatccgcaag
     6241 aaagaaacag gcgcccttta ggacgccgat caggtgtact cgttggccct tgtagtctcg
     6301 agtgatcgtg cgcgcgacgg ctttcacgcg cttctcaatc gcggagcgga gaatcagcac
     6361 cttgctcact tcgcgctcgc cgtttcggat cgtctctggt ctcccctcga cgaatcgctt
     6421 actctaccac cgttgtgtgt tttgcaaggc gcgcggaagg agcaattcca tgatgtcgtc
     6481 gcctgcttgg gaatcgacgc tcgaacaatt tcgcagtgcg gctgcaagca gcgagccaat
     6541 ccccgccgga gtggcggtcg ccgctacgtc cgccagcttc gctcttgggc tgctttcgaa
     6601 agttctgaaa gtcaccggca ggaaaaaagg gttcagcggg gaccttgcga aggtcgagaa
     6661 actagctgag tctgctcgcg ccgagtcgaa gcgcatgatg cagctcgccg aggacgatat
     6721 ggcggcgttc aacgattaca tgatcagcgc gcgcttgccg caaggaacag atcgcgagcg
     6781 ggacgaacgg aatcgggcgg tcaatgcctc ggttcgcaag gcgattgaaa ctcccgtcgc
     6841 ggcggctcgc gcggcggcat ccggcatcag tctctgtggc gatgccctcg acagcgtcca
     6901 cgcgatcgta gccgccgatc tgggcgcagc cgcttcgctc ctagcggggg ccttgcgcgt
     6961 gtttctcctt tgcgccgact cgaatatccg gcaactcgcg ccggatccgc ccgcgtatcg
     7021 cgacgttctc ccaggccggg ccgagtggga agcccgcgcg ttcagccaag ccgatttcgt
     7081 cctgaaaaag gtttcggagg taatcgacgc tgtcaccaag aaaccgtagg gccggtttct
     7141 acgaaagtcc gattattcgt cctgtcttga gatcgatgtc gatctcgtaa tatcccggcc
     7201 gtgtggatag gcccggcatc gtgctcatcg tcccgagcag cggatagagg aacccggctc
     7261 caacgctcgc gcgaacatcc cgcaccggaa ccgtaaatcc cgtaggcgca cctttcaggt
     7321 tcgcgtcgtg gctcaggctc aagtgcgttt tcgccatgca catgggcagt ttgttgaacc
     7381 ctagccgatc gtagagcgca atcttcttct ctgcttccgg cgagtattcg acgcccgctc
     7441 cgccatacat ttcccgcaca atcgtctcga tcttttgctt aatccccatt tccaaaggat
     7501 aaagaaattg aaacttgctc ggtttttcgc atgctgcgat cacagccttg ccaagttcga
     7561 ccgctccagc gccaccctga gcccagtgat ttgacatcac cgcatcgacc gcaccggctt
     7621 tcatggcaat cttccggact agatcgactt ccgccggtgt gtcgtactgg aagcgattga
     7681 cgccgaccac gacggggatt ccatatttac gggcattgtc gatcatcttg atgaggttgg
     7741 agcagccctt ttcgagcagc tcgagatttt cctcagtgta cgccggattc aaaggctgac
     7801 cggcaacgac tttaggtcct ccgccatgca ttttcagagc gcgaatcgtg gccaccatca
     7861 ccacgcaatt cggaaccagt ccgctgtagc ggcatttgat gttgaaaaat ttctccatcc
     7921 cgaggtcggc gccgaatcct gattcggtga gcacgtatcc atcgggccct actaatttca
     7981 atgcgacctg atcggccaca atcgaggaat ttccatgggc gatgttccca aatgggccgg
     8041 cgtgaacgaa cgcgggcgtg ccttcgagcg tttgcatcag gttgggcatg atggtgtctt
     8101 tcatcagcac ggtcagcgcg ccgcccactc cgatgtcgtc cgccgtgatc gcttcgccgg
     8161 ccttgtttgt gccgatgacc atgcgtccaa ggcgctcgcg catatcggcg aggtcggttg
     8221 ccagcgccaa aatcgccatg atttcgctgg ccacggaaat atcgaatccg gttacgcgcg
     8281 tcactccttt ttcctcgggc ccttgcccga tcgtgatctg gcgcaacata cggtcattcg
     8341 tgtctgtcac ccggcgccac gtgatgctat cgacatcgat atccagtcgc gcgaatcggc
     8401 tgcgctcctc gggcgtgagg tcgttgggat tcgttttcgt gatgccaagc tttcgcaacc
     8461 gccgcagcat caccggggaa aatcgccggc tgccgtcctt tgcgggtgga cacaggcgct
     8521 cgaaaagcgt ttcgtcgctc tgcgactttt cgtgaaacca gcgcgtatcg attgccgcag
     8581 ccagcaaatt attcgcagcc gtaatcgcgt gtatgtcgcc cgtaagatgg aggttgaact
     8641 cctccatcgg aatgatttgg ctgtatccgc cgccggccgc gccgcctttg atgccgaatg
     8701 tcggtccctg gctaggctga cggatgcagg tgaaaacttt cttgcccagg tgcgctccga
     8761 tggcctggct caggccgacg gtggtggtgg ttttcccttc gccgagcggc gtgggggtga
     8821 tggctgtgac cacaatgtat tttccgttgg gggtattctt caggcgatct cgaaccgaga
     8881 gatgaacctt ggctttgctt tttccataaa ggtccaattc gtccggcagg atgcctactt
     8941 cggcggcaat tttgtcgatc ggaatcggtg tggcggcctg cgcaatatcg atgtcacttg
     9001 gaacgcgtgc aagtatattt tgcagcttat gaggcattct ctttcctctc tctaatttga
     9061 tgaaaactga ctacgggatt cgagacgcgt ggttgctgaa gcttaccggc gcagtccgct
     9121 cctaaggcta tgaagcaaac aagtaccgta tatggacgct ctcggcattg tcaagccgca
     9181 acctgttccg tcttggcccg ataattctgc catcctgacc ggcagcaagc ggatttgttc
     9241 cctcgggttt tgtgacgggt agaatgagac gtcgcctttt gtttccagca agttacgtgg
     9301 gattctactc ggtagaactg tcgattagaa atgccatccg cggaaagcag ttggcgtctg
     9361 gcgaggatcg agagaacggc gcaggggccg cgccgcgatt ccgatgtgtc catgccttct
     9421 ccttttccgt ggcaaaaagc gcccacgact cacgctaaca cgaaggagat ttagaaatgt
     9481 caacgggtat ctgtggactt acgaattaag aagtggaacc caagatggcg aacggagatc
     9541 ccgttgaagt gggcacaacg atccgaggta tttacacagt cggacaatag aagctcatga
     9601 agcggcccgc aaagggttaa gagtcacatg aaaaaagggc gtccgcagag gcaccctttt
     9661 tcgttgtctg ccgatttgtt ggtctcagct gcagccggag gttgagccgc agttggagca
     9721 cttgtagcag cttccgttcg gcgtcatcaa catgccgcac tcggagcagt agggcgcatc
     9781 ctccatcgcc gcgcgagggc gcgttgtcgt ttcctcgatc gcgccgggta tcgcgaacgc
     9841 accaggggcg acaacggccg ccgccgatcc cgcatcggcg ccgcgctccg gcacagcaac
     9901 atcatcgcga tccagatagt ccggcgagat gaatttgccg ccgagccagc gtccgatgta
     9961 atccactatc gactttgcgt agcgaatctt cggattgccc gtatagcctg aaggttcaaa
    10021 gcgcgagttg cagaatttat ccaccaacgc gcgcaaggga acgccgtact gaagcgaaat
    10081 cgagacggac agcgcgaagg catccataat tccagaaagc gtggagcctt ccttggccat
    10141 cttgatgaag atttcgccgg gcttgccatc gtcgtacatc ccgaccgtaa tgtagccctc
    10201 gtgtccgccc accgagaatt tgtgggtgat ggatctccgc tccgcaggca gcttttcgcg
    10261 ccgcgcgcgc acgaaaagct cttgctgctc cggaccggga accgctggtt gtggggaagc
    10321 ttgaaaagcg ccctgcaaag tcgccggtgc aagcatcacg gcagccttgg cgggcgcggc
    10381 agctttttct tcttcttttt ttccggccgc cgaaagcggt tgcgaacgct tcgagttgtc
    10441 gcggtagatc gccacggcct tcaggccaaa tttccacgat tccatgtaag cctcggcgat
    10501 gtcttccacc gtcgattcct ccggcatatt gatcgtcttg ctgatcgcgc cggaaaggaa
    10561 tggctgagcc gccgccatca tgcgcaaatg gccgcgccag gaaattgatc tgccgccgcc
    10621 cattggcgtc agtgagcaat cgaataccgg caagtgctcg ggtttgagtc cgggcgcgcc
    10681 ttcgatcttg ccctgcgcgt caatgtaatc aacgatctgc gccacctgct ccggcggata
    10741 cccaaggttc atcagcgcct ggggcaccgt gttgttgacg atcttgatta cgcccccgcc
    10801 caccagccgc ttctgtttca ccaacgccag atcgggctcg atgccggtgg tatcgcaatc
    10861 catcatgaag ccgatcgtgc ctgtaggcgc gagaacagat acctgcgaat ttttgtagcc
    10921 gaacttttcg cctaattcga gcgccgcgtc ccacgattcc tgcgcggcgt gcagcagcga
    10981 cggctgcaca ttctcttctt taataggccg cagggaatcg cgatgcatcc gaatcacatc
    11041 tagcatggat tcgcggttgc gcggatagcc gccaaacggc cccatgcgct ccgcgatgcg
    11101 cgcggactgc gcgtaggatt cgcccgtcat cagcgccgta atcacgccgc agacatcgcg
    11161 cccgccatcg gaatcgtagg gcagggccat ggacatcaaa agcgccccca gattcgcata
    11221 gccgatgccg agtgggcgat aatcgtgcga attgcgagcg attttttccg tgggataact
    11281 tgcgttatcg accagaattt cctgcgccgt aatcgttatg tccaccgcgt ggcgaaatcc
    11341 ttccgtatcg aattgcccat tcgttccgag gaatttcaaa agatttaagg atgccaggtt
    11401 gcaagccgtg tcgtcgagga acatatattc cgagcacgga ttcgaggcgt aaatgcgatc
    11461 ggtggcctta gaagtgtgcc aacgattgat ggtcgtatcg tactgcatgc cgggatcgcc
    11521 gcaccgccag gctgattcgg ccatcatctt cattaaatcg cgcgcgcggt gctgatccac
    11581 cggctcgccg tcggtcacgt tgcgagtcca ccagtcgcgg tcttcctcca cggcgcgcat
    11641 gaaatcgtcc gtaacgcgca cggaatgatt cgcattctgg aagaagacgg acgaatacgc
    11701 atcgccatca atcgcggagt cgtacccttg ctcgatcagg acgtgcgcct tgcgctcttc
    11761 cctggttttc gattcgatga actccacgat atccgggtga tccacattga gaatcaccat
    11821 cttcgccgcc cggcgcgtct tgccgccgct cttgattact ccggcgaacg cgtcaaaccc
    11881 cttcatgaag ctcaccggtc ccgaagcgat gcctccgccc gaaagcgact ctttgctacc
    11941 gcgcagcgac gagaagttcg ttcccgtgcc cgagccccac ttgaaaagca tcccttccgt
    12001 ttttgccagg ttcatgatcg attccatatt gtcctggacg gaattgatga aacacgccga
    12061 gcactgcggc ttggcctgta cgcccacgtt gaaccatacc ggcgaattga aagccatctt
    12121 ctgttcgacg agcagatgag tcagctcatc gtggaacgcg tccttggatt ccgcggtggc
    12181 gaaatagccg cccgcctctc cccagcgcac gatggtatcc gctacgcggc tgatgagctg
    12241 gcgcaccgag ttctcgcgct cgggcgaatt cggcttccca tgaaaatatt tagatgtaac
    12301 gatattcgtc gccgtctgcg accacgactt aggaacttcc acattttcct gccggaaaat
    12361 tacctggcct ttatcattcc cgattagggc ggtacgacgc tcccattcga ccctgtcgaa
    12421 gggagacact ttgccgtccg taaaacgccg ctggaaatcc agtccactcg ctcgccgtgc
    12481 gattttttcg gtttgcatgg gagtgttcaa agcagttcga gtctcagcca tctctgcctc
    12541 cgcgccggca gtgctgccgc cgaatagttg gccggggcgc cgtctggggg gcttctctat
    12601 tttactacct ccccgccagc acccggaatg tgtttgcgtg catcgatgga tacacgccga
    12661 tgaaggagtt ttaaacgagc ccagcagtct cgccctaccc cggaacgaaa ccgctgaact
    12721 aaaaaaaaac gaaaagcgcc cctaagcgcg cccttcccgg cgcgatgcag ataccctcgc
    12781 gtcgctttgt gtttccgaac tcggtccggt acgccgtggc gaccgttgac gctgggtgcg
    12841 acgatcgatt caggttttgc cgaatctggg tgcgcagtat cgtgggttgt cgaaatgttg
    12901 tcaaggaaaa ttcgcaacca caccacaaaa tattcggctc tctgtgtcga acccgtctat
    12961 ctatagtggt gtcatcgcgc cacggcctga acacacaaat ctcaggaaat gcctgcggca
    13021 gcacgcgggc cttagtccac acctcaccgt ggacgatttt tctttacacc aacaactact
    13081 tgcgcgattt cgacccctcg tgtaaagatt ttcctcgtat cctcggactc tgcccaccga
    13141 gccgcgtggg cgccaagccc aaatcaatcg attcactcgc ccttc
//
LOCUS       DATNDT010000011         4186 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2866133, whole genome shotgun sequence.
ACCESSION   DATNDT010000011 DATNDT010000000
VERSION     DATNDT010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2866133"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..883
                     /locus_tag="VN788_00470"
     CDS             <1..883
                     /locus_tag="VN788_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HXU19034.1"
                     /translation="PARKANAKILVDGGDPQETLRIKNRVGVVDGQTTNPTLIAKNPE
                     VRQLISSGHKFSSQEELNEYKKIVQAISPLVGDAGVSIEVFADFDTTAEQMFAQGQEM
                     FSWIPNAYVKYPCIHEGLRAAQMSVRKGIRVNMTLCFSQEQAAAVYAATMGSKEPAYV
                     SPFLGRLDDRGENGMDLVANIKKMYQGGDGHVQVLAASIRHLDHLLGSFALNAELITA
                     PGKIWEEWAAASFPVPGKDFAYKAVDKNGNRLKPIPYREIDLKSKWESYDIRHDLTTK
                     GIQQFVADYKSTLKQSA"
     gene            897..2123
                     /locus_tag="VN788_00475"
     CDS             897..2123
                     /locus_tag="VN788_00475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HXU19035.1"
                     /translation="MSSSKYVILGGGMVAGYAAKEMVERGLKSGELTIISADDTLPYE
                     RPPLSKGFLSGKDTEAGILINPPDWYKEKGIEVKLQTVVEGVDLQKKRLVASSSQDFQ
                     FENLLIATGCRARRLESPGNNLANVFYLRSMDDSRKIRSCAANAKQAVVIGGGFIGME
                     VASVLAQKNIHTTMIVREDRVWERAFTPEMSAFFERYYTARGVQLIKHANVTALEGKK
                     TVSAAVLDDRRKIPCDVVVAGVGAAPVTDLFAKTELSNNNGIAVNEYLETAKPGFYAA
                     GDVANYPDPIFKKRRRVEHWDNAVSHGHHWARVILGDRHPFVHVPYFFSDVFDLSYEL
                     WGDSEGATQTVVRGEMESSKFSVWWLKQDRLVAAFVMSRPDEERTVAPEWIKSNQLVS
                     PKRLADQAKAIQDAAA"
     gene            complement(2535..2765)
                     /locus_tag="VN788_00480"
     CDS             complement(2535..2765)
                     /locus_tag="VN788_00480"
                     /inference="COORDINATES: protein motif:HMM:NF022608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HXU19036.1"
                     /translation="MTKTFKRGDHVSWNSEAGRVSGVILKKVVADVRIKGYVHHASKQ
                     EPQYLIKSDKTDHVAIHKGKALRRLKPRKRRG"
     gene            complement(2831..4108)
                     /locus_tag="VN788_00485"
     CDS             complement(2831..4108)
                     /locus_tag="VN788_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HXU19037.1"
                     /translation="MLDNLRRVIVDYYRNEASPQNGLIADRTQPGSPSSIAATGMGLS
                     VYIVAVERNLLSRADAVDRTLAVLRFLHSSHQGPEPDATGYKGFYYHFLDMQTGRRAI
                     QCELSTIDTAILMAGVLTAASYFTAKNEKESEIRKLAELLYRRIDWRWALDKGTTISH
                     GWNPESGFLSHRWDTAYSEAIILYALALGSPTFPIDPQGYEDWISTFEVKKVYDTEYL
                     YAGPLFIHQMSHLWVDFRGIHDDFNRKTGIDYFENSRRATYVHRQYAIENPMRFAHYS
                     KYVWGLTASDGPGDTVLEVNGVRRAFFSYAARGAPFGPDDGTVSPWAVATSLPFAPDI
                     VIPTIHHIIERLKLLGHSPYGFVASYNPTYPEHTDNFDAWASPWIFGLNEGPVVMMIE
                     NFHTELVWKTVQRCPYFVEGLKRAGFRGGWLDR"
BASE COUNT          983 a         1136 c         1119 g          948 t
ORIGIN      
        1 gcctgcacgc aaagctaacg cgaagatttt agtagatggc ggtgatcccc aggaaacgct
       61 tcgaataaaa aatcgagtgg gcgttgtgga cggccaaact acgaatccca ctctgattgc
      121 gaagaatccg gaagttcggc agctgatttc ctccggccac aaattcagct ctcaagaaga
      181 gctcaacgaa tacaagaaga tcgtacaggc catttcaccg ttggtcggtg acgcgggagt
      241 gtccatcgag gttttcgcag atttcgatac caccgccgaa cagatgtttg ctcaaggcca
      301 ggaaatgttt tcctggattc cgaatgccta tgtgaaatat ccctgcattc atgaaggcct
      361 gcgcgcggcg caaatgtccg tgcgcaaagg catccgcgtc aatatgacgc tctgtttttc
      421 gcaagagcag gcggcagcgg tctatgccgc tacgatggga tcgaaagaac cagcctatgt
      481 gtcgccgttc ctcggcaggc ttgacgatcg cggtgaaaat gggatggatc tcgtcgcaaa
      541 cataaaaaaa atgtaccagg gcggtgatgg ccacgttcaa gtgcttgcgg caagcattcg
      601 tcatcttgac cacctcctcg gttcatttgc tttgaacgct gaactcatca ctgcgccggg
      661 caaaatatgg gaagagtggg cggccgcgtc tttccctgtg ccgggcaaag atttcgccta
      721 taaagctgtc gataaaaatg gcaaccggtt aaaaccgatc ccctacagag aaatcgattt
      781 gaaatcaaaa tgggaaagtt acgacattcg ccacgaccta accacaaaag gcattcagca
      841 atttgtcgcc gattacaaaa gtacgctgaa gcaatcggcc tgaaaatcga ggatctatga
      901 gttcgagcaa atacgtcatt cttggcggcg gcatggttgc cggttatgcg gctaaggaga
      961 tggtcgagcg gggcctgaag tccggcgagc taacaattat ttctgcggac gataccttgc
     1021 cttacgagcg ccctccgctg tcgaagggct tcttatccgg caaagatacc gaggcgggca
     1081 ttctgattaa cccgcctgac tggtataagg aaaaaggaat cgaagttaag ctgcaaactg
     1141 tggtcgaagg cgttgatctg caaaagaagc gccttgtcgc ctcttcctcc caggattttc
     1201 aattcgaaaa tcttttgatc gccactggat gccgcgcacg aaggcttgag tcgccaggta
     1261 acaatctggc caacgttttc tatctccgct ccatggacga ttcgcgcaaa atacgctcgt
     1321 gtgccgcaaa cgcgaagcag gccgtcgtta tcggcggggg tttcatcggc atggaagtcg
     1381 catccgtgct ggcgcagaaa aatattcaca ccaccatgat cgtccgggag gatcgagtct
     1441 gggagcgcgc gtttacccct gaaatgtccg ctttctttga acgctactat accgcccgcg
     1501 gagtacaact catcaagcac gcaaatgtaa ccgcgctcga aggcaagaag acggtcagcg
     1561 cggcagtact cgacgatcgc cgcaagattc cctgtgacgt ggttgttgcc ggagtcggcg
     1621 ccgctcccgt aaccgacctc ttcgcgaaaa ccgagctttc aaataacaac ggcattgccg
     1681 tgaatgaata tctcgaaacc gccaagcctg gattctatgc cgcaggcgat gtggcaaatt
     1741 atcccgatcc gattttcaag aagcgccggc gagttgagca ttgggacaat gcggtttcgc
     1801 acggtcacca ctgggcccgc gtgatcctcg gtgatcgaca ccctttcgtc cacgtccctt
     1861 atttcttctc ggacgttttc gacctgtcgt acgaactctg gggagattcc gaaggagcaa
     1921 cccagaccgt cgtgagagga gagatggaga gttccaagtt tagcgtctgg tggctcaagc
     1981 aagatcgact ggttgcggcg ttcgtcatga gccgtccaga cgaagagcgc acagtagcgc
     2041 ccgaatggat taaatccaac cagctcgttt cacccaagcg cctcgccgat caagccaaag
     2101 ccatccaaga cgccgcggcg tagcgctggc gtcccgccgg cgtttttctt cgccttgcgc
     2161 ggtccggtgg aagcgcgggc ctttcaggcc cgcgaaaagc gagccacata atcatggctt
     2221 tagccatggg ccgtgtttgc tagcccacga cgaacgcatt cctcgtcgtg agccttgggc
     2281 ttcgtagggc cacggcacgc cgtgcccggc aaccatgctc ggcaccctgt caactattcg
     2341 tgatgatgag cggagcatcc cggctctttc tcttgcgcga ccgggtggcg cactctgcgc
     2401 aggcacaaag aggagcgctt tcttgttgtc agcgtcggag gcgcgattgt aattagcccg
     2461 gcacgtagcg aagcggagtg ccgggtccgc gaacgaaaat caaaagtccc ggagggacga
     2521 ttgattcagg cggctcagcc tcttcgcttc cgaggcttca aacgcctcag cgctttgcct
     2581 ttatgaatcg cgacatgatc ggttttgtca ctcttaatga ggtattgcgg ttcttgtttt
     2641 gatgcatggt gcacgtagcc cttaattcgt acgtccgcaa caaccttctt gaggatcacg
     2701 ccgctcactc tgccagcttc tgaattccat gaaacatggt caccgcgctt gaacgtcttg
     2761 gtcatcggag tagtcactat tgtgaagcaa gcggacctga ggattcgaga tcgaagctat
     2821 taaagaacgc tcaacggtca agccagccac cgcgaaaacc tgcgcgctta aggccctcta
     2881 caaagtacgg gcatcgctgg acagtcttcc agacaagctc cgtgtgaaag ttctcaatca
     2941 tcataacaac cggcccttca tttaagccaa atatccacgg agacgcccaa gcgtcgaaat
     3001 tgtcggtgtg ctcaggatag gttggattgt aactggcaac aaacccgtac ggactatgcc
     3061 ccagcaattt aagccgctcg ataatgtgat ggatcgttgg gatcacgata tcaggtgcga
     3121 atgggagaga tgtcgctaca gcccagggtg agacggtgcc atcatccggt ccaaacggag
     3181 ctcctcgtgc cgcataagaa aaaaatgctc ttcggacacc gttcacctca agaacggtat
     3241 ctccgggtcc gtcgctcgcg gtcagtcccc acacgtattt gctatagtgc gcaaaccgca
     3301 ttggattctc aattgcatac tgcctgtgaa cataggtggc ccggcggctg ttctcaaaat
     3361 aatcgatccc cgtcttcctg ttgaagtcgt catgtatgcc gcgaaaatcc acccaaaggt
     3421 gggacatctg atgaatgaat aatggtccgg catacaggta ttctgtgtcg tagactttct
     3481 tcacttcaaa agttgagatc cagtcttcgt acccctgcgg atcgatggga aaggttggtg
     3541 accctagggc cagcgcgtaa agaatgatgg cttcgctata ggctgtatcc caccggtgcg
     3601 aaagaaatcc agactcagga ttccaaccgt gagaaatcgt cgtcccttta tcgagcgccc
     3661 atcgccaatc gatacgtcgg tagagaagtt cggcgagctt ccgtatctcg ctttcctttt
     3721 cattcttcgc cgtgaaataa cttgccgccg taagaacacc cgccattaat atggcggtat
     3781 caatcgtcga taattcgcat tgtatggctc ggcgtccggt ctgcatgtcg aggaagtggt
     3841 agtagaaccc tttgtacccc gtggcatccg gttctggacc ctgatggctg gaatgcaaaa
     3901 acctcaaaac cgccagagtc ctatcgactg cgtccgctcg tgaaagtagg tttcgctcta
     3961 ctgccacaat atagacgctg agccccattc ccgtggccgc tatgctcgaa ggcgacccgg
     4021 gttgggtcct atccgcgatc aggccgtttt gagggctggc ctcattgcga taataatcga
     4081 cgatgacacg ccggagattg tcgagcattt ctgaatcggt aacctggctg gtcgccggcc
     4141 ctttcgctcc gtatttcggc tggcattcga gagcgggcgg atgata
//
LOCUS       DATNDT010000012         7495 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_548471, whole genome shotgun sequence.
ACCESSION   DATNDT010000012 DATNDT010000000
VERSION     DATNDT010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7495
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_548471"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..2025
                     /gene="guaA"
                     /locus_tag="VN788_00490"
     CDS             466..2025
                     /gene="guaA"
                     /locus_tag="VN788_00490"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HXU19038.1"
                     /translation="MSTKQGIVVLDFGGQYTQLIARRIREQQVFSAILPCTASIEEIR
                     AQEPVGIVLSGGPSSVYDQDAPRCEAKTLDLGVPMLGICYGMQWLTRTLGGKVERAER
                     REYGPAQLNIGRDSKLFAGLPTQLKIWNSHGDHVSGLPEGFHITARTNNAVAAIENPA
                     KQFYGVEFHPEVNHTDRGTEILNNFVFGICGARRNWNRAGFIAEVVESIRTRAAGARA
                     ICALSGGVDSTVAAALVDRAIGDRLTNVFVDTGLLRRDEFEDTLALLRGRLGLNVIGV
                     DASERFLTKLSGVTDPEQKRKIIGGEFIAVFAEEQRKLRADAREGPPVKFLVQGTLYP
                     DVIESISVKGPSAVIKSHHNVGGLPKDMPFELIEPLRDLFKDEVRQIGRELGLPEEIL
                     VKHPFPGPGLAVRLLGAVTPEKLDILRAADAVVVDEIRRAGLYEKVWQAFAVLLPVQS
                     VGVMGDGRTYAATIVVRVVESLDAMTADWSRLPMEVLERISTRIVNEVAGVNRVVYDI
                     SSKPPSTIEWE"
     gene            complement(2126..2497)
                     /locus_tag="VN788_00495"
     CDS             complement(2126..2497)
                     /locus_tag="VN788_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU19039.1"
                     /translation="MIIGDRIRLLREDKNLSQGDIEKRTGLLRCYISRVENGHTVPAI
                     ETLEKLARAMEVPLYQLFYDGEEPPALPNLPKRKSADDIAWGSRGKDARYLGRFRRLL
                     GRLPEGDRRLLLYVAQKMANR"
     gene            2579..3259
                     /locus_tag="VN788_00500"
     CDS             2579..3259
                     /locus_tag="VN788_00500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU19040.1"
                     /translation="MTRVSKTMAASAPALALSVDREEAKRDDRELVRLSQAGTPAAFE
                     ELVRRHQQRVFALIGGILRCAEDVEDVAQQVFLKAYLGIKKFDQRAAFSTWLYKIAVN
                     ECWDYLRKKKVRPLVYEANLSEEQVSRLDGIVSADRPPTNANSRAETRELLDRLLATL
                     PEQDQQLLVLKEMEGFSVQELAEILHLNVNTVKVRLFRARGRIMDVYRRRAISAHKAD
                     GTAPQRKR"
     gene            3266..3766
                     /locus_tag="VN788_00505"
     CDS             3266..3766
                     /locus_tag="VN788_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXU19041.1"
                     /translation="MADTNVRTACAKYEPVLEDYLEGVLDQDGAEAARRHLQNCAGCN
                     AAFEHAAGSVKLLQVAEPSEGPDAAFARNVMARIRTAEQERTAERAGFWQPVVALGWR
                     FAATATLAVAVLVSCDIGLSHYARINVPTVRSVEGNLFAPEPARPPANVDEVLMMVAE
                     TGHGKQ"
     gene            3753..4163
                     /locus_tag="VN788_00510"
     CDS             3753..4163
                     /locus_tag="VN788_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19042.1"
                     /translation="MANNKARREATALVFLVFVLGVIFGGVGDHLWRVRAANQQPINT
                     KPTRHEVMNGLTQQLQLTPDQQKRIGVAIDDTRAKWQALYAPLDASREQIRQQGRANI
                     RAVLTPEQKAKFDDFLRRLDEQRKKEAAAAATGH"
     gene            4246..5040
                     /locus_tag="VN788_00515"
     CDS             4246..5040
                     /locus_tag="VN788_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013314708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU19043.1"
                     /translation="MTATIRLDGVHKTYDLGEIQVHALRGVSMEIFAGEFVAVMGASG
                     SGKSTLMNILGCLDKPSRGHYFLDGKDVSGLTKSELARIRNHKIGFVFQQFNLLARTS
                     ALENVELPTIYAGVPLEERAKRAREALDRVGLSDRAGHFPSQLSGGQQQRVAIARALV
                     NNPSLLLADEPTGNLDSRTSVEIMDILQGLNERHGLTVVIVTHEPDIAQYAKRALEFR
                     DGKMRKDVAIQNRLIAAEVLPTLPVLDEDDVEAVADAAPAAPSDGH"
     gene            complement(5047..5526)
                     /locus_tag="VN788_00520"
     CDS             complement(5047..5526)
                     /locus_tag="VN788_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008290886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HXU19044.1"
                     /translation="MSVNKAILVGRLGRDPETRYTSGGQAVCNFSVATDSTYKDRNGE
                     RQKRTEWHRIVLWGKLAEIAQQYLKKGQLVYIEGRLQTREWEDKRDGQKRSTTEIVGS
                     EMRMLTSRAEGAAAAGVGASSSSSPSPARSADADFEGAAPPDEHGPSPEISDEDIPF"
     gene            5671..6105
                     /locus_tag="VN788_00525"
     CDS             5671..6105
                     /locus_tag="VN788_00525"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HXU19045.1"
                     /translation="MSQPQTFRNHAKFVPAFHFFVAPVFLLNIVWSIVRVVRSFSFGT
                     IVSLLVAIALFLLALTARMFALAVQDRVIRLEMRLRMQQALPADLTPRIPEFTVSQLV
                     ALRFASDGELPDLARKVLQENLTERKAIKAMVRNWQPDHLRA"
     gene            complement(6148..7101)
                     /locus_tag="VN788_00530"
     CDS             complement(6148..7101)
                     /locus_tag="VN788_00530"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU19046.1"
                     /translation="MRTRWVVLTFAAVLLGAIGGAMLAPQPASAVSRDMIELQQQVAQ
                     LLQGQQDMRSAMDSNNATIKTLVQQSLDSVNKLSAEMSSVQKSVQEVQANTGARIDTM
                     AQQTQGLSDNLQDVQARVGKLSQQLSDAQNLLQSIDGKLSNQPGATGTPGATGAPGSP
                     GATGGPTTSNDTPGGNPPPGMAPISADTLYQNALRDYTSGKYDLARQEFSDYIKNFPS
                     NDLASNSQFYLGEIAYAQGDFKQAIGAYNAVLVNYPRSFKLAASQLKKGMAELESGER
                     AAGIRDLRTVESHFPGSDESRRAHAKLREVGATTTSRAPAR"
     gene            complement(7290..>7495)
                     /locus_tag="VN788_00535"
     CDS             complement(7290..>7495)
                     /locus_tag="VN788_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXU19047.1"
                     /translation="CDERGSTEYNLGLGQRRAEAAKNFLISLGITADRMETTSWGKER
                     PVCSEHTEQCWQLNRRAHFVLTQ"
BASE COUNT         1590 a         2006 c         2378 g         1521 t
ORIGIN      
        1 gacggcttgg cggaggatga aacgaaggag cgggcgcagg gcgacgctcg catcgcgccg
       61 ctgatcgccg ggaagaagat tgtgaaggta gtagtggtgc cgaaaaagct ggtcaatatt
      121 gttttggagt aggagaaaac cgaaaagcct caccccgtta aggcggggtg agctacatga
      181 aatcgagcgt ggggacggag tccgagaata cggataaaat ctttacgctg gggcaccgaa
      241 cggcgcaagt agttgttggt gtaaagaaaa atcgtccacg gtgagtgtgg acttttggcg
      301 tttatgggga gcaaatacca aggctaagtc tgcaccactg agacgcagag aacgcagaga
      361 cggaatggcg gatcgagtcg ttcacggtga cgatcgagag gcgagacaaa aagcgcaacg
      421 cgtccgagaa acggcaatcg ggcgtggcga tcacgaggag ggcagttgag caccaagcaa
      481 ggaatcgtgg ttctggactt cggcgggcaa tatacacaac tgattgcgcg tcgaatccgc
      541 gaacagcagg tgttctcggc gatccttccg tgcacggcgt cgattgagga aattcgcgcg
      601 caggagccgg tgggcatcgt cctatcgggc ggaccgagtt ccgtttacga tcaggacgcg
      661 ccccgatgcg aagcgaaaac gcttgatctt ggggttccga tgctgggaat ttgttatggc
      721 atgcaatggc tgacgcgcac gctgggcgga aaagtggagc gagcggagcg ccgcgaatac
      781 gggcccgcgc aattgaatat tggccgggac tccaaactgt ttgcgggact tcccacgcaa
      841 ctgaaaatct ggaatagcca tggcgatcac gtgtccggtt tgcccgaagg cttccacatc
      901 accgcgcgga cgaacaatgc agttgcagcg atcgagaatc ccgccaaaca attctacggc
      961 gtggaatttc atccggaagt gaatcacacc gatcgcggga cggagattct aaacaatttt
     1021 gtgttcggca tctgcggcgc gcgccgcaat tggaaccgcg ccggattcat tgccgaggtc
     1081 gtcgagagca tacgcacccg ggctgcgggc gcgagggcta tctgcgccct gagcggcggg
     1141 gtggattcga ccgtggcagc agctctggta gatagggcca tcggggaccg tttgaccaac
     1201 gttttcgtgg atactgggct cttgcggcgc gatgagtttg aggacacgct ggcgctcttg
     1261 cggggacgac ttggtttgaa tgtgatcggc gtcgacgcat cggagcggtt cttgacaaaa
     1321 ttgagcggtg ttaccgatcc ggagcaaaag cgaaaaatca ttggcggcga attcatcgcc
     1381 gtgttcgccg aggaacagcg caagctgcgg gccgatgcgc gagaaggtcc acctgtaaaa
     1441 tttctggtgc aggggacgct gtatcccgat gtgatcgagt cgatttccgt aaaaggacct
     1501 tcggcagtaa tcaagtcgca ccataacgtg ggcggattgc cgaaggacat gcccttcgag
     1561 ctgatcgagc cgctgcggga tctattcaag gatgaagtgc gccaaatcgg aagggaactg
     1621 ggattgccgg aggagatatt agtaaagcat cctttcccgg gtcccggatt agcggttcgc
     1681 cttctcggag cggtaactcc cgagaaactt gatattctga gggcagccga cgcagtggtg
     1741 gttgacgaaa ttcgccgcgc cggtttgtac gagaaggttt ggcaagcctt tgccgtgctg
     1801 ctgccggtgc aaagcgtggg cgtgatggga gacggccgca cctatgcagc gacgatcgtg
     1861 gtgcgcgtgg ttgaatccct ggacgcgatg acggcggact ggtcgcgcct gccgatggaa
     1921 gtgctggagc gcatttccac gcgaatcgtg aacgaggtgg cgggcgtgaa ccgggtggtc
     1981 tatgacatca gttcgaagcc gccgagcacg atcgagtggg agtagaatta gagaagcgaa
     2041 tagaggtcgc ccagagacac caagagcatg agaagagtcg cttcacaacg ggggacgatt
     2101 gattccaaag gaaatagcga tccggttacc ggttcgccat tttttgggct acatagagca
     2161 aaagccgacg atctccctcg ggcaagcggc cgaggagtcg acgaaaacgg cctaagtagc
     2221 gagcatcttt tcccctgctg ccccaggcga tgtcgtccgc gctcttccgc ttgggaagat
     2281 tgggaagtgc gggaggttct tcgccatcat agaagagctg gtagagtgga acttccatcg
     2341 cgcgcgccag cttttccagg gtctcgatcg ccggaacggt atgtccgttc tcgacgcgcg
     2401 agatgtagca gcgaagcaga cctgtccgct tttcgatgtc gccctgcgag agatttttgt
     2461 cttcacgtag cagtcgaata cggtcgccga ttatcatggc gggtattgtt gcatggacta
     2521 gtgttagtcg caagagcaaa aggaaaaagc gaggattttt cagcgtaacc gaagaccaat
     2581 gacacgcgtc tcaaagacca tggcagctag tgctccggca ctcgctctat ccgtcgatag
     2641 ggaagaggca aagcgggacg accgcgaact ggtacggttg tctcaagccg gaacgccagc
     2701 agcgttcgag gaactggtgc ggaggcatca gcagcgcgtc ttcgcgctga ttggcggaat
     2761 cctgcggtgc gcggaagacg tggaggatgt agcgcagcaa gtttttctga aggcgtacct
     2821 tggaatcaag aaattcgatc agcgagcggc gttttccacg tggctctaca aaatcgcagt
     2881 gaacgagtgc tgggattatt tgaggaaaaa gaaagtgcgg ccactggttt atgaggcgaa
     2941 tttatcggag gagcaagtct cgcggctgga cggtatcgtt tccgcggacc gcccgccgac
     3001 gaacgcgaat tcgcgggccg aaactcgaga attgctcgat cgactgctgg ccacactccc
     3061 cgagcaggac cagcagctgc tggtcttgaa ggagatggaa ggattttccg tacaggagtt
     3121 ggcagagatt ctgcacctca acgtcaatac cgtgaaagtg cgactgttcc gtgcgcgagg
     3181 gcgaatcatg gatgtctatc ggcgccgggc gatctcggcg cacaaagcgg atggtactgc
     3241 gccgcaaagg aagaggtaaa tagccatggc ggatactaac gtcaggacgg cttgtgcgaa
     3301 gtacgaaccg gttctcgagg attacctcga gggcgtactc gatcaggacg gggcggaagc
     3361 cgcgagacgg cacttgcaaa actgcgcggg ctgcaacgcg gcgttcgaac acgctgctgg
     3421 gagcgtaaaa ctgctgcagg tcgcggagcc gtcagaagga ccggatgccg catttgcgag
     3481 gaacgtgatg gcacggattc gcaccgccga acaggagcgg accgccgagc gtgcgggctt
     3541 ctggcagccg gttgtagcct tgggatggcg attcgcagcg acggccacat tggccgtggc
     3601 cgtgttggta agttgcgata tcggcttgag ccattatgcg cggatcaacg tgccgacggt
     3661 tcgctccgtg gaaggcaatc tctttgcgcc tgagccggcc aggcctcccg cgaatgttga
     3721 cgaagttcta atgatggtgg cggaaacagg ccatggcaaa caataaagca cgccgcgaag
     3781 ctacggccct cgtgtttctt gtattcgtgc taggggtgat ttttggcggc gttggcgacc
     3841 acttgtggcg cgtgcgcgcg gccaaccagc agccgatcaa tacgaagccg actcgccacg
     3901 aggtgatgaa cggccttacc cagcagcttc aactgacgcc ggaccagcag aagcgcatag
     3961 gcgttgcaat tgacgatacg cgcgcgaaat ggcaggcact ctatgcgccg ctcgatgcgt
     4021 ccagggagca gatacgccag cagggccggg cgaatatccg ggcggtgctg acgccggagc
     4081 agaaggcaaa attcgacgac ttcctgaggc gtctggatga gcagcgcaag aaagaggccg
     4141 ccgctgccgc caccgggcat taacggcgca gctccgacca acgccatgga tgaacgcgcc
     4201 gccaaggacc gaactcctgc aattccctcc aggccaacag aggcagtgac cgccacgatt
     4261 cgtctcgatg gcgtgcataa aacttacgac ctaggcgaaa tccaggtaca cgccctgcgc
     4321 ggcgtatcga tggagatttt cgctggagaa ttcgtggccg tgatgggagc gtcgggttcg
     4381 ggaaaatcga cgctgatgaa tattttgggc tgcctcgata agcccagtcg cggacattat
     4441 ttcctggatg gcaaggacgt gtctgggctc acaaaaagcg agcttgcgag gattcgcaac
     4501 cacaagatag gtttcgtgtt tcaacagttc aatcttttgg cgcggacttc ggcgcttgaa
     4561 aatgtggagt tacccacgat ttatgcgggc gttccgctgg aagagcgcgc caagcgagcg
     4621 cgagaagctc tggatcgcgt gggcctctcc gatcgcgccg gccattttcc ttcgcagcta
     4681 tcgggcggcc agcagcagcg agtggcgatt gctcgcgcgc ttgtcaataa tccttcgctg
     4741 cttttggctg acgagccgac gggaaatctt gatagtcgca ccagcgtcga gatcatggac
     4801 attttgcaag gcctgaacga gcggcacggg ctgaccgtcg tgatcgtcac tcacgagccg
     4861 gatatcgcgc agtacgcgaa gcgcgcgctt gaattccgcg atggcaagat gcggaaggat
     4921 gtggcgattc agaatcgctt gatcgccgcg gaagtgctac cgactttgcc cgtgctggat
     4981 gaagacgacg tggaagcggt tgcggacgca gcccctgccg cgccgtcgga tggtcactaa
     5041 agccggctag aaggggatgt cttcgtcgga aatttccggg cttgggccat gctcgtccgg
     5101 cggtgcggcg ccttcgaagt ccgcgtcggc ggaacgagcc gggctcgggc tgctcgaaga
     5161 gctggctccg acaccagctg cagcagcgcc ctcggcacgg gaggtcagca tccgcatctc
     5221 gctgccgacg atttcggtgg ttgagcgttt ttgcccatcg cgcttgtctt cccactctcg
     5281 cgtctgcaag cgcccttcga tgtacacgag ttggcccttc ttcagatact gttgagcgat
     5341 ttcagccagc tttccccaca aaacgatgcg atgccactcg gtgcgctttt ggcgctcgcc
     5401 gttgcggtcc ttgtaggttg aatcggtggc gacggaaaaa ttgcagacgg cctggccacc
     5461 ggaggtataa cgcgtctcag gatcgcgccc caggcgccca accaggattg ctttgttcac
     5521 ggacatgggc aggagtatag cccaaacgaa gccgggaaga aatacacgga gggggttcaa
     5581 cacgccagat cagcgcgtcg cggctagttt cgtgacacaa tcgagaactg cttaggtttc
     5641 atcggccgag ttttcaaaag ggaggtaagc gtgagccaac cgcaaacttt cagaaaccat
     5701 gcaaaatttg tgccagcttt tcacttcttt gtggcgcccg ttttcctgct caatattgtg
     5761 tggtcaatcg tgcgtgtagt tcgcagtttc tcgttcggaa cgatcgtctc gttgctcgtt
     5821 gcaatcgcgc tgttcctgct cgcgttgacc gctcggatgt ttgcgctcgc ggtgcaggac
     5881 cgcgtgatcc gtctggaaat gcgattgcgc atgcagcagg cgcttccagc ggacttaacg
     5941 ccgcgaattc ccgaattcac agtgagccaa ctggtggctc tgcgattcgc gagcgacggg
     6001 gaactgccgg atctcgcgcg aaaagttctg caggagaatt tgacagagcg caaagcgatc
     6061 aaagcgatgg ttcgaaattg gcagccggac cacttgcggg catagagggg cagtcaacgc
     6121 tacaacggaa cgccgaggcc ttggagatta gcgcgcagga gcccgcgaag ttgtggtggc
     6181 gccgacctcg cgcagtttgg cgtgagcgcg ccgcgattcg tccgacccgg ggaagtggct
     6241 ctccacggtt cgcagatcgc gaatgccggc cgctcgctcg ccggattcaa gttccgccat
     6301 ccctttcttg agctgcgacg ccgccaattt gaaactcctc ggataattca ccagcaccgc
     6361 gttgtaggcg ccgattgctt gcttgaagtc cccttgcgcg taagcaattt cgcccagata
     6421 aaactgcgag ttggaggcca agtcgttcga ggggaaattt ttgatgtagt cggaaaactc
     6481 ctggcgcgcc agatcgtact tgccgctcgt gtagtccctc agtgcgtttt ggtaaagcgt
     6541 atcggccgag atgggggcca tccctggcgg cggatttcca cctggcgtgt cattcgacgt
     6601 tgtgggtcct cccgtcgcgc ctggagagcc cggcgccccg gtcgcgcccg gcgttccggt
     6661 tgcgcccggc tgattcgaca gtttcccgtc gatgctctga agcaggttct gggcatcgga
     6721 gagttgttgc gataatttgc caacgcgcgc ctgtacgtct tgcaaattgt cggagagtcc
     6781 ctgggtctgc tgggccatcg tgtcaatgcg ggcccccgta ttcgcctgca cctcttgcac
     6841 ggacttttgt accgagctca tttcagcgct gagcttgttg acggaatcga gcgattgctg
     6901 aacgagcgtc ttgatggttg cattgttgct atccatcgcg ctgcgcatgt cctgctgtcc
     6961 ctgcagaagt tgggcaacct gctgctgaag ctcgatcata tcgcgtgaaa cggcactggc
     7021 gggctgcgga gccaacatcg ccccgccgat agcgcctaac agcaccgctg cgaatgtgag
     7081 cacaacccat cgtgtgcgca tagggtatcc tcccggaaaa attcacccgg ccgattcggc
     7141 caagccggtt tgcaccaccc cgcagtccgc gaggtggcaa acccttctct atattattaa
     7201 cgttgccgcg cccatgcggt actaaaaagg tggctccgcg gcaaatgtac cgtagcagtc
     7261 gcccgagcag cggcgacgat tgccgcaccc tattgagtca aaacgaaatg cgcgcggcgg
     7321 ttcaattgcc agcactgctc cgtgtgctcg ctgcaaaccg gacgctcttt gccccagcta
     7381 gttgtttcca tccgatcggc ggtgatgccg agcgagatca agaagttctt tgccgcctcg
     7441 gcgcgccgct ggcctagacc aagattgtat tcggttgagc cgcgctcgtc gcaat
//
LOCUS       DATNDT010000013         4361 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_262343, whole genome shotgun sequence.
ACCESSION   DATNDT010000013 DATNDT010000000
VERSION     DATNDT010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262343"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            147..449
                     /locus_tag="VN788_00540"
     CDS             147..449
                     /locus_tag="VN788_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014400136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HXU19048.1"
                     /translation="MSEAAKQQQVQIKADEKELLGQYSNLVVVHHNAEEFTLNFVYMF
                     PTVPQGKLVASLIVNPAHAKRLLHALQENVSRYEAQFGKLPEGPGPDQGPNVGFVQ"
     gene            complement(475..816)
                     /locus_tag="VN788_00545"
     CDS             complement(475..816)
                     /locus_tag="VN788_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19049.1"
                     /translation="MRIGISTTKAKNIATIRRETRVPMEVGVDISGHARVPGTEATFT
                     QNVSARGARVLSVRRWKINDHLVITMRTGCFQSVGRVAYCQPMPHAGYALGIEFLAPR
                     GQWVVSEGMQH"
     gene            complement(838..2136)
                     /locus_tag="VN788_00550"
     CDS             complement(838..2136)
                     /locus_tag="VN788_00550"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXU19050.1"
                     /translation="MGITDNLRSHFRFRLVLIGAIALAAYCILLGFLRVSSYRGEPLL
                     DAYVEMAGSLIAFTFAANAMVRFRGLHDRISLILAFGFVVAGLIEAGTSMAFYRGMLV
                     TPTGGEHIALGWLAGRTLLGVLLVAALVVERRIPISREPGKEIAGATLVVGAVAYLTS
                     VFYFMIPGTPKIEPGALVPRPWDLVPALIFAAATVGFAWRLRRATAALDRALVIAAGL
                     NVICHLTMSQSQLVLDAPYMVAHVLMVLSYVVVLGGTLLDNADLFDKVSHMAASDSLT
                     GLANHRRLLESMEMEIERCRRSRRGFAVLLFDLDDLKKINDRYGHLTGSRAIQRVGMV
                     LRDSSRAIDTAARYGGDEFALVLPECGQREADLAAARICERVAEDGLEPHLSISVGHA
                     IFPVDGTSIEQLLGAADLVLYKMKGRKVSKVRLSQVVACL"
     gene            2459..3133
                     /gene="msrA"
                     /locus_tag="VN788_00555"
     CDS             2459..3133
                     /gene="msrA"
                     /locus_tag="VN788_00555"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HXU19051.1"
                     /translation="MSARLLLTLGFAFSILACAAIAAPERPPIPPAKVDAPRAHAAGK
                     ETAVFAGGCFWGTQAVFERVKGVLNTAAGYSGGSASTATYNQVVTEKTGHAESVEVVY
                     DPSQITYGQLLRIFFSVAHDPTELNRQGPDVGPSYRSAIFYANDEQKRIAKAYIAQLD
                     AAKVFPKPIVTEVTPLKGFYRAEDYHQDYALKNPTNPYIKICDLPKISALKQQFPELF
                     VDYKGK"
     gene            complement(3188..3409)
                     /locus_tag="VN788_00560"
     CDS             complement(3188..3409)
                     /locus_tag="VN788_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HXU19052.1"
                     /translation="MQKPIVYTIRGCDACVKLLRKWDAEGVVYEERRVEQDQKLLDQA
                     RTYGDMVPIVVWPDGRVEQGFEGNLGCFI"
     gene            complement(3426..4061)
                     /locus_tag="VN788_00565"
     CDS             complement(3426..4061)
                     /locus_tag="VN788_00565"
                     /inference="COORDINATES: protein motif:HMM:NF025946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXU19053.1"
                     /translation="MLEATTVVTPARFEQGLRYADFLAQATVNRDKFELYYKDSPLSA
                     EDISFFKKAAAAPNGPARILALAEAWCGDVYRELPSVARIAEATGMDLRVFLRDENPD
                     IMDEFLSNDGRARAIPVFVFYTSETRYIAHFTERSAGAHAELAAIMDDVKRQLKLPAT
                     ATFATVPEAEKQAFLREVIAKIRPRFPDWQKESIKEMRALLATALKLPNRG"
BASE COUNT          946 a         1374 c         1138 g          903 t
ORIGIN      
        1 tgattgttcc ccgttcattt agctgcacag gattttctca caggccgctt gcgatcggcg
       61 gcgcgaattg cccgaaataa ccgcccgcaa agcatcgcgg cccgtgatgg ctctgctata
      121 gtcaagagct gccaaggagg aaaagaatgt ctgaagcggc gaagcagcag caggtgcaga
      181 tcaaggccga tgaaaaggaa ttgctcggcc aatattcaaa tctggtcgtt gtccatcaca
      241 acgccgagga attcacgctg aatttcgttt acatgtttcc caccgtgcct cagggaaaat
      301 tagtggccag cctgatcgtg aaccccgcgc atgccaagcg gctcctccac gcgcttcagg
      361 agaacgtgtc gcggtatgag gcgcaattcg gtaaattacc ggaaggtccg gggcccgatc
      421 agggacccaa cgtgggattt gtgcagtagt ctcggcgagc gcgcgaagcg aaggctaatg
      481 ctgcatgcct tcggaaacca cccactgccc gcgcggcgcc agaaattcga ttcccagtgc
      541 gtaacctgcg tgaggcatcg gctgacaata agccacgcgc ccaactgatt ggaagcatcc
      601 cgtccgcatg gtaattacca gatgatcgtt tattttccag cggcgcacgc tcaaaacgcg
      661 ggctccccgc gcgctcacgt tctgggtaaa cgtagcttcg gttcccggca cgcgtgcgtg
      721 tccgcttata tccactccca cttccatcgg cacgcgggtt tcgcggcgaa ttgtcgcgat
      781 gtttttggcc ttggtggtgg agattcctat ccgcatgatc aatggccttc gcttcaatta
      841 caggcaagct acaacttggc tcaaccgcac cttgctaact tttcgcccct tcattttgta
      901 aagcaccagg tcggcggctc ccaaaagctg ctcgatggat gtgccgtcga cggggaaaat
      961 agcgtggcca acgctgattg aaagatgtgg ttcgaggccg tcttcagcca cgcgctcgca
     1021 aattcgtgcc gccgccaagt ccgcttcccg ctggccgcat tccggcaaca ccagcgcgaa
     1081 ctcgtcgccg ccgtatcgcg ccgccgtgtc gatcgcgcgg ctgctgtctc gcagcaccat
     1141 gcccacgcgc tggatggcgc ggcttccggt caaatggccg tacctgtcgt ttattttttt
     1201 cagatcgtcg aggtcaaaca gcagcaccgc aaaacctcgg cgcgaccgcc ggcagcgttc
     1261 aatctccatt tccatcgact ccagcaggcg ccgatggttg gccagtcccg tcaacgaatc
     1321 ggacgcagcc atgtggctca ctttgtcgaa tagatcggcg ttgtccagga gtgtgccgcc
     1381 caagaccacc acgtagctca aaaccatcag gacgtgagcg accatgtacg gcgcatccag
     1441 caccagttgc gattggctca tcgtaaggtg acagatcacg ttcagccccg cggcaatcac
     1501 caaggcccgg tcgagcgccg cggttgcgcg ccgcagcctc catgcgaatc ccacggtggc
     1561 ggccgcaaaa atcaatgcgg gcaccaagtc ccatggacgc ggcaccagcg cgcccggttc
     1621 aatcttcggc gtgccgggga tcatgaagta aaaaacgctg gtgaggtaag ccacagcgcc
     1681 gaccaccagc gtcgcgcccg caatttcttt tcccggctcg cgcgatatcg gaattcgccg
     1741 ctccaccacc agcgccgcta ccaataacac tccgagcaac gtgcgccccg ccagccagcc
     1801 cagcgcaatg tgctctcctc cagtcggagt caccagcatt ccccggtaga aggccatgct
     1861 cgtccccgcc tcgatcaagc cggccaccac gaacccgaat gccaggatca gcgagattcg
     1921 atcgtgcaat ccgcgaaacc gcaccatggc gttcgccgca aacgtgaacg cgatcaggct
     1981 tcccgccatc tcgacatagg catccagcag cggctcgccg cggtacgatg aaacgcggag
     2041 gaaccccaaa aggatgcagt aggccgccag cgcaatcgcc ccaatcagaa cgagacgaaa
     2101 gcggaaatga ctgcgcaaat tgtctgtaat acccactctg cccccggaga tgacacttct
     2161 ccccgcaaat aaacctgcaa gtaatagacc ttctaggacc actgcgtaac gtaatataac
     2221 tcccgtgttt acatggtaat gcggacctac cgccaaaaag agagatcggg cgcagtcgca
     2281 aaatgacacc caggccccta acggggcagt tgccgtcgcg aattgagata tcgcgccccc
     2341 ggccggccgg cgtagttttt cgcaaaaaca ccccgcacct tacgattcga ttccgcctgg
     2401 acgtatacta gatttgatgg aaaagcatcc tatgaatgaa gttgtgagga gcctgtcaat
     2461 gtcagcccgt ttgcttttga cccttggatt tgccttctcg attctggctt gcgccgcgat
     2521 tgctgcgccc gagcggcctc ccattcctcc cgcgaaggtg gacgcaccac gcgcccacgc
     2581 cgctggaaag gagacggccg tttttgccgg cggctgtttc tggggcactc aggcggtgtt
     2641 cgaacgcgtg aaaggcgtcc tcaataccgc ggcgggctat tctggcggtt cggcatccac
     2701 cgccacttac aatcaggttg tcaccgaaaa aaccggacat gccgagtcgg tcgaagtggt
     2761 ttacgatcca tcgcaaatca cctatggaca gttgcttcgt atcttttttt ccgtggctca
     2821 cgatccgacc gaactcaacc gccagggacc ggacgtcggt ccatcctacc gttccgccat
     2881 cttttacgcc aacgatgagc agaagcgcat tgccaaggct tacatcgcgc agctagatgc
     2941 cgccaaagtt tttccgaagc caatcgtcac cgaagtcact ccgctgaaag gtttctatcg
     3001 cgccgaggat tatcaccagg attacgccct gaagaatccc accaatcctt acatcaaaat
     3061 ttgcgacctg ccgaagatca gcgcgttgaa gcagcagttt cccgagttgt ttgtcgatta
     3121 caaaggaaaa taaagcgcac cgggtggctt atatcgctag gtcgcatgct aagaacaaat
     3181 cgcagtttca aataaagcag ccaagattgc cttcgaatcc ctgttccact cttccatctg
     3241 gccaaaccac gatggggacc atatcgccgt aagtgcgtgc ttggtccaac aatttttgat
     3301 cctgctccac gcgccgttct tcgtaaacca cgccctcggc atcccacttt cgcagaagtt
     3361 tcacgcacgc atcgcagccg cggattgtgt acacaattgg cttttgcatc tccgcgagcc
     3421 tcggttcatc cgcgatttgg cagcttcagg gcagtcgcca gcagcgcgcg catctctttg
     3481 atggattctt tctgccaatc cggaaaccgc ggacgaattt tcgcgatcac ctctcgaaga
     3541 aacgcttgct tctcggcctc cggcaccgtc gcaaatgtag ccgtcgcagg caacttcagt
     3601 tggcgcttca cgtcatccat gatcgcggcc agctccgcat gcgcgcccgc cgaccgttct
     3661 gtgaaatgcg cgatatagcg agtctcgctg gtgtaaaaca caaacacagg tatcgcgcgc
     3721 gctctcccgt cattgctgag aaattcgtcc atgatgtcgg gattttcatc gcggagaaat
     3781 acgcgcaggt ccattcccgt cgcctccgca attcgcgcca cgctcggcag ttcgcgataa
     3841 acatcgccgc accacgcttc cgccagcgcc aatatcctcg ccggaccgtt cggcgcagcc
     3901 gcagccttct tgaaaaaaga aatgtcttcg gctgaaagcg gcgagtcctt gtaatagagt
     3961 tcgaatttat cgcgattcac agtcgcctgc gccagaaaat ccgcatagcg cagcccttgc
     4021 tcgaaccgcg cgggtgtcac aacggtggtc gcttccagca tgttctcttc tccaatcggc
     4081 tgagaggata aatcttatca cacggcactc gcaccgccgt ccgaccatga tgcgaacatc
     4141 tgggttgcga tctggagagc agtgcaggcg acccgttttg accttgcgac tttctggcgc
     4201 ccccatcccg cggcgtcacg aagtggtgac ggcattgaca cttagcttgg cagtatggta
     4261 tcgcagatgc ccatgttccg gttctcggcg cggggccttt tccccgtccg gaccgccctg
     4321 ggtcatgtaa gtccccgtcc atgggcaaca atgaaaatcc c
//
LOCUS       DATNDT010000014         8712 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3132412, whole genome shotgun sequence.
ACCESSION   DATNDT010000014 DATNDT010000000
VERSION     DATNDT010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8712
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3132412"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1219
                     /locus_tag="VN788_00570"
     CDS             <1..1219
                     /locus_tag="VN788_00570"
                     /EC_number="4.1.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013411266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004611 - phosphoenolpyruvate
                     carboxykinase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0017076 - purine nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (GTP)"
                     /protein_id="HXU19054.1"
                     /translation="PEEKLIWSVGSGYGGNALLGKKCFALRIASWMAHEQGWMAEHML
                     ILGLEDPSGKVTYMAAAFPSACGKTNLAMMVSTLETQGYRVWTVGDDIAWMHIASDGT
                     LRAINPEAGFFGVAPGTNVKTNPNVISAAHHNTIFTNVAITAKSHEPWWEGMDGAPPD
                     GLISWKGAPWKPGSETAAHPNARYTVPASQSSSISDRWEDPAGVPISAIIFGGRRAHL
                     APLVYESHNWQHGVFIGATMASETTAAATGAVGVTRRDPMAMLPFCGYNMGDYFGHWL
                     EMGSKLKNPPKIFHVNWFRRDADGKFLWPGYGENVRVLKWMLDRIEGRARATETPIGY
                     VPEPSSLTLDGLSIPRETLNELLRVDPGNWMEEDQAIAKFLEKFGDRLPPALSQEQKS
                     LTARLSSSSVATK"
     gene            1232..2374
                     /locus_tag="VN788_00575"
     CDS             1232..2374
                     /locus_tag="VN788_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase/methylcitrate synthase"
                     /protein_id="HXU19055.1"
                     /translation="MSPNATANPRGLEGVVAAQTRLSHVDGQNGVLIVGGYSIEDLVA
                     HATFEEGAHLLWRDRLPNREELAKLSSEMAELRELPPLALEVIRAAAKAPPIDALRMA
                     CATLSLGLKDPNAITPEADFAAAMRLTACFPTIVAAHARLSKGKEPIAPRKNLSLAAN
                     FLYMVHGRELDPAAAHALDAYWVTVMDHGMNASTFTARVIASTRSDLVSSVTGALGAL
                     KGPLHGGAPGPVLDMLLDIKTADRAEPWIRNELSQGRRIMGFGHRVYKVRDPRAEVLS
                     RVAERLQGAQIENRQLYELARHVEQTALRVLAEVKPGRNLRTNVEFYTALVLQAIGLE
                     PAQFVAMFACGRTAGWCAHVIEQHKEDHLIRPQSEYIGPTGLKMDR"
     gene            2540..3901
                     /locus_tag="VN788_00580"
     CDS             2540..3901
                     /locus_tag="VN788_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19056.1"
                     /translation="MPVAALALLFLPPLSIAQQPPTARESSHNGSQAQSDAGASQMPG
                     MKMGASGQMSMEPHNFIQEITAHATSGTSAEPNSTPMPMLMAVKNSWTLMFHANVFIL
                     DEQQSGARGGDKLFSTNWFMGMAKHNAGPGVLTIRSMLTLEPATVSERRYPLLFQQGE
                     TAFGVPIADGQHPHDFVMELGALYDVKLGDKGLLTFYFAPVGDPAIGPTAYPHRESAS
                     ENPVGALGHHQEDSTHIAADVATVGFTYGIARIEASGFHGREPNEFRWDIDQGKIDSW
                     AMRLTVQPEKNWSGQYSYARITSPEGLFPDENQERMTASIMYNRPLNNGNWANTALWG
                     RTRSLADNAILNSYLFESMVRFRKRNRAWTRIENADRTNELLVGENPLPPGFRELPIG
                     HVQGYTFGYDRDFNFIPDLESALGAQITTYGVPSTLTPIYGSHPIGAAIFIRLRPFSA
                     KHH"
     gene            complement(3907..4770)
                     /locus_tag="VN788_00585"
     CDS             complement(3907..4770)
                     /locus_tag="VN788_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19057.1"
                     /translation="MRGRHENMLIRRPPALLLLLVIAVALPVAAQETPGTSNAPAQAN
                     HPDEPEANPGRPTISTPATLTPVGYFQFETGYLGAWHSPEFSSQSSVNEVVKFSVTRR
                     IELLAADQPFAYSRAGKQTGWTTGDVALGVQGVVYQGEGARPTLAMSYFHRLYAGDAP
                     DVDIGSNRNSVLILASADVKGFHYDTNFIFNEVIDSGIHRTQFGQTLSVSHALPKKFG
                     ISGEIWHFTQPFLRSNAVGNLWALNYNAQRNLVLDGGFNRGLTSTSTRWEVFIGFTYL
                     LPRKLSLRPGA"
     gene            complement(4767..5183)
                     /locus_tag="VN788_00590"
     CDS             complement(4767..5183)
                     /locus_tag="VN788_00590"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXU19058.1"
                     /translation="MYEKILVTLDGTPSDRAIIEHVKELARLAHSRLVLLHVADGWAA
                     RTYGHDAVSPEIAEDTAYLEKVRAEFQSVGIQAECELAYGSPSKEIIKWIEKKGCDLV
                     AMSTHGHRFLADVFLGTTASRVQHGIRVPVLLLKAK"
     gene            complement(5215..6597)
                     /locus_tag="VN788_00595"
     CDS             complement(5215..6597)
                     /locus_tag="VN788_00595"
                     /inference="COORDINATES: protein motif:HMM:NF001923.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXU19059.1"
                     /translation="MATKAPQTGHVSLEGIHRTVHVPHHQAGFWEQWRAFVGPAILVS
                     VGYMDPGNWGTDLQGGAQFKYALLWVVALASVMAIFMQIIAARLGVATGKDLAQCCRD
                     WYPRWTRWPNWVLSEVAIGACDLAEVLGSAVALNLLFHIPLLWAVIITGLDVLLLLAL
                     QGFGIRTIEAVVLVLVATIGVCYFIEIFVLPETRASFLEMGRALVSPHLHQAGMIYVA
                     IGIIGATVMPHNLYLHSALVQSRQLQKDDLSVRRAIQFNTIDSIAALVVAFCVNAAIL
                     VLAATVFFGKESVAVPGGQVVMFGANSDWIRVAYLTLAPLLGTAFAGTLFALALLASG
                     QSSTITGTLAGQVVMEGFMHWRIQPWVRRLITRTLAILPAVFIIGLRGDSSVTDLLTL
                     SQVVLALQLPFAMFPLLHFTSSRKRMGVWKNGWFLLAVGWASAILITAIAIYGLPDSI
                     RTAWQIITGR"
     gene            complement(6848..7429)
                     /locus_tag="VN788_00600"
     CDS             complement(6848..7429)
                     /locus_tag="VN788_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU19060.1"
                     /translation="MNSKPGTHNQEHLDLVFLFALQALSPREIASVEAQVLSCEDCQH
                     EIETLRPIVRSFVAWPTDVLRPAESLWGRLAKRIAGEAATQPFVPPVQSPDKPKWEEA
                     APGIHVKILARNAEIESVSMLVRLDPGTDYPAHQHAGTEELHLLHGVLKVDDRTLYPG
                     DFIHSEVGSVDHRVWSETGCTCFLMTSTEDSLF"
     gene            complement(7426..8052)
                     /locus_tag="VN788_00605"
     CDS             complement(7426..8052)
                     /locus_tag="VN788_00605"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU19061.1"
                     /translation="MQPPATKRLGDVLYADKSKFRVPEEEWLKLLRAISGGDQLALHS
                     LFEQTHRIVFTLIVRITMNRETAEEVTLDVFYDVWRKASTYDPANGSVVGWVMNQARS
                     RAIDRLRFDQRKKRVNTYPHSLRPTTDIVDPQQACLFEEQTRHLQGALEALTAEERKA
                     IETAFFSELTYEQTARDLNEPLGTVKTRIRSGLEKLREALGRKVKATG"
BASE COUNT         1913 a         2563 c         2444 g         1792 t
ORIGIN      
        1 tcccgaggaa aaattgattt ggagcgttgg ctcgggttac ggcggcaacg ccctgctcgg
       61 gaaaaaatgt ttcgcgcttc gcattgcaag ctggatggct catgaacagg gttggatggc
      121 cgaacacatg ctcatcctcg gcctcgaaga cccctccggc aaagtcacgt atatggccgc
      181 cgcttttccc agcgcttgcg gcaaaaccaa tttagccatg atggtttcca cgctcgaaac
      241 ccagggctat cgcgtgtgga cagtcggcga cgacatcgcg tggatgcaca tcgcgtccga
      301 cggcacgttg cgcgccatca accctgaagc tggatttttc ggcgtcgccc ccggcaccaa
      361 tgtaaaaacc aatcccaacg tcatctccgc cgcgcatcac aacaccattt tcaccaacgt
      421 cgccatcacc gcaaaatcgc atgagccttg gtgggaaggc atggacggcg cgccgcccga
      481 tggcctaata agctggaagg gcgccccgtg gaaaccgggc tcggaaaccg ccgcgcaccc
      541 gaacgctcgc tacaccgttc ccgcttcgca atcctcctcg atttccgatc gttgggaaga
      601 tcccgcgggc gttccaattt ccgcgatcat cttcggaggc cgccgcgcgc atctcgctcc
      661 cttggtctac gaatcgcata actggcagca cggagttttc atcggcgcga ccatggcttc
      721 ggaaaccacg gctgccgcga cgggcgcggt gggcgttacg cgccgcgatc ccatggctat
      781 gctcccgttc tgcggctaca acatgggcga ttatttcgga cattggctcg agatgggatc
      841 gaagctcaaa aaccctccga agatttttca cgtgaattgg ttccgccgcg acgcggatgg
      901 aaaatttctc tggcccggct atggcgaaaa cgttcgcgtg ctgaaatgga tgctcgatcg
      961 catcgaaggt cgtgcgcgcg ccacggaaac gccgataggt tatgtgccgg agccgtcatc
     1021 gctcacgctc gacggactct ccatcccgcg cgaaactctc aacgaacttc tgcgcgtcga
     1081 tcccggcaat tggatggagg aagatcaagc catcgcaaaa ttccttgaaa aattcgggga
     1141 tcgcctccca cccgctctca gccaggaaca aaaatctctc accgcgcgac tttccagttc
     1201 gtcggtcgcc accaaatagc gaacgaatcg catgagcccc aacgccactg ccaacccgcg
     1261 cggactcgaa ggcgtcgtcg ccgcacagac acggttgagc cacgtggacg gccagaatgg
     1321 cgtgctcatt gtcggcggct attccattga agatctcgtc gcccacgcca cgtttgaaga
     1381 aggcgcgcat cttctctggc gagaccgctt gccaaatcgc gaagaactcg cgaagctttc
     1441 atccgaaatg gccgaattgc gcgagttgcc tccgcttgcg ctggaagtaa ttcgcgccgc
     1501 ggcaaaagct cctccgattg acgcgttgcg catggcctgc gccacgcttt cgctcggcct
     1561 caaagacccg aacgccatca cgcccgaagc tgattttgcg gcagcaatgc ggctcaccgc
     1621 gtgctttccc accatcgttg ccgcgcacgc gcggctgtca aaagggaaag aaccgattgc
     1681 tccgcgaaaa aatctctcgc tggcggcaaa ttttctgtac atggttcacg gacgcgaact
     1741 cgatcccgct gccgcgcacg cgctggacgc atattgggtc accgtgatgg atcacggcat
     1801 gaatgcttct acgttcacgg cgcgcgtgat cgccagcacg cgctccgact tggtgagcag
     1861 cgtcaccggc gcgctgggcg cgctgaaagg cccgctgcac ggcggtgcac ccggccccgt
     1921 cctcgatatg ttgctcgaca taaaaacagc cgatcgcgcc gaaccttgga ttcgcaacga
     1981 actttcgcaa ggtcgccgca tcatgggatt cggacatcgc gtttacaaag tgcgcgatcc
     2041 gcgcgccgaa gttttaagtc gcgttgccga gcgcctgcag ggcgcgcaaa tcgaaaatcg
     2101 ccagctctac gaactcgcgc gtcacgttga acagactgcg ctgcgcgtgc tcgctgaagt
     2161 gaagccgggg cgcaacctgc gcaccaacgt cgagttttat accgcgctgg tgcttcaggc
     2221 cattggcctg gagcccgcgc agttcgtggc catgttcgcg tgcggacgta cggcgggatg
     2281 gtgcgctcac gtgatcgagc agcacaaaga ggatcacctg atacgcccgc aatccgaata
     2341 tatcggcccc accggcctga agatggatcg ctaacggctg aattttccaa cgtcaccgca
     2401 atttgtcttc gccgtatcca cggcgaatgc caggcgaata aaatagcgca aaagaatctt
     2461 ctagtttggc gcatttattc cttctaactc gatgcaatta ggcgcctcat ttcgctattt
     2521 ttcgcaccgt tggagcgccg tgccggtcgc cgcgctcgca cttttgtttt tgcctcctct
     2581 cagcatcgcc cagcagcctc cgaccgcgag ggaaagttcg cacaatggca gccaggcgca
     2641 atccgacgcg ggcgcgagcc agatgcccgg catgaaaatg ggtgcgagcg gacaaatgag
     2701 catggagccg cacaatttca ttcaggaaat aacggctcac gccacttcag gcaccagcgc
     2761 cgaaccgaat tccactccca tgccgatgtt gatggcggtg aaaaattcct ggacgctgat
     2821 gtttcacgcc aatgttttta ttctcgacga acagcaatcc ggcgcgcgcg gcggcgacaa
     2881 acttttctcg acgaattggt tcatgggcat ggccaagcac aatgccggcc ccggcgtgct
     2941 cacgattcgc tcaatgctta ctctcgagcc ggcaacggtc agcgagcgcc gttacccatt
     3001 acttttccaa caaggcgaaa cagcgttcgg tgtgcccatt gccgacggac agcatcctca
     3061 cgatttcgtc atggagcttg gcgcgctcta cgacgtcaag ctgggcgaca agggcctgct
     3121 cacattttac ttcgcgccgg tgggcgatcc ggcgattggt cccacggcgt atccgcatcg
     3181 cgaatcggca tcggaaaatc ctgtcggcgc gctcggccat catcaggaag attcgactca
     3241 cattgccgcg gatgtcgcca cggtgggatt tacttacggc atcgcgcgga tcgaagcttc
     3301 gggatttcat ggacgcgagc caaacgaatt tcgctgggac atcgatcagg gaaagatcga
     3361 ttcgtgggcg atgcgcctca cggtgcaacc ggagaaaaat tggagcggcc aatactccta
     3421 cgcgcgcatc accagtcccg aggggctttt cccggacgaa aatcaggaac gcatgaccgc
     3481 gtccatcatg tacaaccggc cgcttaacaa tggcaattgg gccaacaccg ccctatgggg
     3541 acgcacgcga tcgcttgcag acaacgccat tcttaatagc tatcttttcg agtcgatggt
     3601 gcgatttcgc aagcgcaatc gcgcgtggac gcgcatcgaa aacgccgatc gcaccaatga
     3661 attgctagtg ggagaaaatc cgttgccgcc cggcttcaga gagcttccaa tcgggcacgt
     3721 gcaaggttac accttcggct acgaccgtga tttcaatttc attcccgacc tagaatccgc
     3781 gctgggagcg caaatcacca cgtacggtgt gccaagcacc ctcactccaa tttacggatc
     3841 gcacccaatc ggcgccgcca tcttcattcg attgcgcccc ttctctgcaa agcaccacta
     3901 gcgcgattat gcgccgggac gcagcgagag tttgcgcggc agcaggtagg tgaacccaat
     3961 aaatacttcc cagcgcgttg acgtgctggt gaggccgcga ttgaagccgc catcgagaac
     4021 gagatttctt tgcgcgttgt aattcagggc ccacaaattg ccgacggcat tgctgcggag
     4081 aaaaggctgg gtaaagtgcc agatttctcc ggagatcccg aatttctttg ggagcgcgtg
     4141 cgaaacggag agcgtctgtc cgaattgggt gcgatgaatt ccgctgtcaa tcacctcgtt
     4201 gaaaatgaaa ttggtatcgt agtgaaagcc ttttacatcg gcgctagcga gaatcagcac
     4261 ggagtttctg ttgctgccga tatcgacatc gggtgcatcg cctgcgtaca aacggtgaaa
     4321 ataactcatc gcaagtgtgg ggcgcgctcc ttctccctga tagacaacgc cttgcactcc
     4381 cagcgccacg tctcccgtag tccatccagt ttgctttccg gcgcgagaat aggcaaacgg
     4441 ttgatccgcc gccagcaatt cgatccggcg cgttacagaa aacttcacca cttcattgac
     4501 acttgattgc gaagaaaatt cgggcgagtg ccacgcgccc aagtacccag tttcaaattg
     4561 aaaatagcca actggagtga gagtggcagg ggtggagatg gtaggcctcc ctggattggc
     4621 ttcgggttca tctggatggt tcgcttgcgc cggcgcgttt gaggttccgg gcgtctcctg
     4681 ggccgcgacg ggaagcgcga cggcgataac caaaagcaac aggagtgcgg gcgggcgccg
     4741 aatcagcata ttttcgtggc gccccctcat ttcgctttca gaagaaggac ggggactcga
     4801 atgccgtgct gcacacggct ggctgtggtg cctagaaaaa cgtcggctag gaaacggtgt
     4861 ccatgggtgc tcatggcaac caagtcgcag ccctttttct caatccattt gatgatttct
     4921 ttcgaaggcg agccgtatgc gagctcgcac tcggcttgaa tgcccacgga ttgaaactcc
     4981 gcgcggactt tttcgaggta ggcagtgtct tctgcaatct cggggctgac tgcgtcgtgg
     5041 ccataggtcc tcgccgccca gccatcggcc acatgaagca gcaccaggcg gctgtgcgcg
     5101 agcctggcga gttctttgac gtgttcgata attgcgcgat cgctcggcgt gccgtccaag
     5161 gtcaccagaa tcttttcata catggctttt cctcttctgt acgcacgtca cggactacct
     5221 gcctgtgatt atttgccacg cagttctgat cgagtccggc aggccatata tcgctatggc
     5281 ggtgatcaga attgcgctgg cccaaccgac tgccaacaaa aaccaaccgt tcttccaaac
     5341 tcccattcgc ttgcgggaac tggtgaaatg cagcagcgga aacatggcga agggaagctg
     5401 tagcgcgagc actacctggc tgagggtcag cagatcggtg acgctgctat caccgcgaag
     5461 tccgataatg aagaccgcgg gaagaatagc gagcgtgcgg gtaataagac gccgcaccca
     5521 cggctggatt cgccagtgca tgaagccttc catcaccact tgcccagcga gcgtgccggt
     5581 gatagtgctg ctctggccgc tggccagaag cgcgagagcg aacagcgtgc cggcaaatgc
     5641 ggttcccagc agcggagcca gcgtgagata ggcaacccga atccaatcgc tgttcgcgcc
     5701 gaacatcacc acctgcccac cgggtacagc cacgctttcc ttgccgaaaa aaaccgtcgc
     5761 agcgagcacc aggatcgcag cgttgacgca gaaagcgaca accaaggctg cgatcgagtc
     5821 gatggtgttg aattggatgg cgcggcgaac ggacaagtcg tctttttgca attggcgact
     5881 ctggaccaga gcagagtgca ggtacagatt gtggggcatt acggtagcgc cgatgatgcc
     5941 aatggcaacg taaatcattc ccgcctggtg aaggtgcggc gataccagcg cgcgtcccat
     6001 ctcgagaaag ctggcgcggg tttccggaag cacgaaaatc tcgatgaagt agcagacgcc
     6061 gatggtggca acgagcacaa ggacgacggc ctcgatggtg cgtatgccaa atccctgcaa
     6121 ggccaacagc agcagcacgt ccaaaccagt gatgatgaca gcccacaaca gcgggatatg
     6181 gaaaagcaag ttgagggcta cggcgctgcc gagcacttcc gccaagtcgc acgcgccgat
     6241 agccacttcg ctcaacaccc aattgggcca gcgagtccag cggggatacc aatcgcgaca
     6301 gcactgagcg agatctttgc ccgtggccac gcccaaccgc gcggcaataa tttgcatgaa
     6361 gatggccatc acgctggcca gcgcgaccac ccacaaaagc gcatacttga actgagcgcc
     6421 gccttgcagg tcggtgcccc agttgccggg gtccatgtag cctacgctga cgagaatcgc
     6481 gggtccgaca aacgcgcgcc actgttccca aaagccagcc tgatgatgcg gcacgtggac
     6541 ggtgcggtgg atgccctcca aggacacgtg accggtttgt ggagccttcg ttgccatcta
     6601 tcctttatgt gttggggcga gtatagtatg aaccagatct cgcagttttc cgctcgtagc
     6661 cccctcgatt tgcttttgag aacagcccct atggtcggct agacttgcag aaaatccgat
     6721 actttaaaga aggctcgcgc gcagacggcg actttcacgc gcggttaacg aacggtcgct
     6781 gtttcttggg aggccatttt cgcgaaaacg gcttcgagtt cgctctcgcg gtagcgagct
     6841 gatcagatca gaaaagggag tcttcagtcg aggtcatcag aaaacaagta cagccggttt
     6901 cactccacac acgatgatcg acgctaccta cctcgctgtg gatgaaatcg ccggggtata
     6961 gcgttctgtc atcaaccttc aatacgccgt ggagaagatg caactcctca gtgccagcat
     7021 gctgatgggc aggatagtcg gttcccggat cgagccggac caacatgctc acactttcga
     7081 tttccgcgtt cctggcgagt attttgacgt ggatacctgg cgccgcttct tcccacttcg
     7141 gtttatcagg ggactgcacg ggaggcacga agggctgtgt ggccgcttcg ccggcgatgc
     7201 gcttcgcgag ccgaccccac agcgattccg ccggccgcag tacgtcggtg ggccaggcga
     7261 caaacgatct cacaatcgga cgcagcgtct cgatttcgtg ctggcaatcc tcgcatgaca
     7321 ggacttgagc ttcaaccgaa gcgatctcgc gcggcgaaag cgcctgcagg gcgaacagaa
     7381 atacgagatc cagatgttcc tgattgtgcg taccgggttt tgagttcatc ccgtcgcctt
     7441 cactttccgt cccagcgcct ctcgaagttt ctccaggccg gagcggatcc gcgtcttcac
     7501 ggttccaagc ggctcattca agtctcttgc cgtctgttca taagtcaatt cagagaaaaa
     7561 tgcggtttcg attgcctttc gctcttccgc agtcagggcc tcgagcgccc cctggagatg
     7621 acgggtctgc tcttcgaaca gacaggcttg ttgaggatca acaatgtctg ttgtcggccg
     7681 caagctgtgc ggatacgtat tcactctctt cttcctctga tcgaaccgca ggcgatcaat
     7741 cgctctgctg cgcgcctgat tcatgaccca tccgacgacg gatccgttgg cagggtcata
     7801 ggtcgaggct tttcgccaga cgtcatagaa aacgtcaagc gtaacttcct ctgcggtttc
     7861 tcgattcata gttattcgca cgatcaacgt aaagacgatt cggtgcgttt gctcgaatag
     7921 cgaatgtagt gcgagctggt cacctccgga gatggctcga agtagcttta gccactcctc
     7981 ttctggtaca cgaaactttg acttatcggc atagagaaca tccccgagcc tctttgtcgc
     8041 cggcggctgc acggggagat catgccgggg tcaagcttca cagtcaagac acagcgtccc
     8101 tgttcctcgg gccataatgt agaggcctct ctatctgaaa agaacgccaa aggtgcgccg
     8161 ccggattggc tggaaacaat gaaatatagc cgtatccccg tgcctgaaag aaattcctgc
     8221 tagaacacaa atccaagaac tgattgttga cgttatttaa agttgcaggg gcgtttggat
     8281 gagcgcggca cctgaccgtc tagggctgtc ttttgacaca tgcgctgctg ctcggccgac
     8341 agtggattga ggctgtccgc aatcgtagtt gtggcgtagc gatcgcatat ccttagtacg
     8401 gcttggactg gcaacgaggg aatacatgca ctatcacatc cgctggtcag atacgaaact
     8461 tgattgggaa ggcttcagta cacgtgaaca agctgagcag gcggccagac acctagcacg
     8521 tccaggcgag aggttcactc tcgaacatgt cgaggacgag acttgtatac aatgccagaa
     8581 aatatccaaa cgcgtgttag ggaacgatga aaacggcggg gctgaagtga gttcgtagag
     8641 cggcgctctt cgtacctccg gcggcaaccg tgcccgtcgg ggaacaccgc ctccatatct
     8701 ccggcaggac gc
//
LOCUS       DATNDT010000015         5910 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1721470, whole genome shotgun sequence.
ACCESSION   DATNDT010000015 DATNDT010000000
VERSION     DATNDT010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5910
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1721470"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..839)
                     /locus_tag="VN788_00610"
     CDS             complement(<1..839)
                     /locus_tag="VN788_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXU19062.1"
                     /translation="MAKVLIPTPLRQYAEKQSAVDLNGATVADVLAALTSRYGELKRH
                     LYTDEGRLRSFVNVYVNDEDIRFLDKDKTGLQDGDTVSIVPSIAGGTAAVADPVAAPV
                     TLSKEEILRYSRHLIIPEVGMDGQLKLKQAKVLLIGAGGLGAPLGLYLAAAGVGRIGM
                     VDFDTVDFTNLQRQVIHGTKDVGRKKLDSAAESMLDINPHVQIDRHETALTSENALEI
                     LKDYDIVIDGTDNFPTRYLVNDACVLLGKPNVYGSIFRFEGQATVFAYEDGPCYRCLY
                     PEPP"
     gene            complement(832..1251)
                     /locus_tag="VN788_00615"
     CDS             complement(832..1251)
                     /locus_tag="VN788_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HXU19063.1"
                     /translation="MIRIEGKAWSAMVAHAESTFPNECCGAMLGVSDGVEKQVSVASP
                     IENAYQGAQNARYELRPEDLLAAEREARKQGMDLIGIFHSHPDCDAYFSETDLKNSCP
                     WYSFVVLSIKGGKFDHANSFLPNLDQTAAEKEELIYG"
     gene            complement(1238..2194)
                     /locus_tag="VN788_00620"
     CDS             complement(1238..2194)
                     /locus_tag="VN788_00620"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXU19064.1"
                     /translation="MPLATAAAPLSKTESTNLLGQIGNTPLLRLDKLTRGLPGIEIYG
                     KAEYFNPGGSVKDRAALNMILEGERSGQLTRDKIILDATSGNTGLAYAMIAAAKGYRV
                     RLCLPRNASHERKQMLKAYGAETILTDPAEGSDGAIRKCRDVYAADPESYFYPDQYNN
                     PANWRAHYETTGPEIVAQTQGRITHFVACMGTSGTFMGVSRRLRQEVPGIRVISAQPS
                     SGFHGLEGLKHMPTAIVPGIYDPDLADDNLWIETEDAYAMTRKLGREEGILVGISSGA
                     NLVGAMSVARRLVERGESGVIVTVLCDGAGKYLSEPFWNDPD"
     gene            complement(2327..3166)
                     /locus_tag="VN788_00625"
     CDS             complement(2327..3166)
                     /locus_tag="VN788_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19065.1"
                     /translation="MQPTLAKLTKEQKKAEREEAEDLITQRGMGKKAFAHLSKPDFIR
                     ELRNRVNDPKLINQGHLDFCGPAAILHAMAKDSPLEYATFALDLFFSGKAAVRGWKVD
                     VSHILDQNIPTSLDPEVTSIGACDWVTMAGVRANVGFGALTSVNIQIQGALPFEIVSC
                     FKSLGYQNVLNETYSTILWKADEKNLKQASELWSQGYRVVFCVNGNMFKDPTAQSLKP
                     NHFCTLKSAVRIANSVTCRVWQWGINDKEPTTAGSSIHVDLPKDQFIAAYFGFVAAGD
                     RRL"
     gene            complement(3218..3676)
                     /locus_tag="VN788_00630"
     CDS             complement(3218..3676)
                     /locus_tag="VN788_00630"
                     /inference="COORDINATES: protein motif:HMM:NF022479.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HXU19066.1"
                     /translation="MLTGTMAGLPQTVAAAVTPVVMVSAAAVLILGLNSKHQSMSDRV
                     RTLAVEFRQASEGRQRTIAAQAGLFQRRMAHVANGQRCLYSAVVCFLVMVLLIVFAPG
                     RISWTSFAFVLFVAGSCLILAAVILEILELALANRTIQIELADVINRPGP"
     gene            3817..>5910
                     /locus_tag="VN788_00635"
     CDS             3817..>5910
                     /locus_tag="VN788_00635"
                     /inference="COORDINATES: protein motif:HMM:NF019135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXU19067.1"
                     /translation="MTGAAIFSLLALSSSGTALGLIPDRALTQYLHRIWQVPQGLPQG
                     TIYSIAQTRDGYLWLGTQTGLVRFDGVHFAPFESSGGAAPGNVWVRSLLEDGNGNLWA
                     GTIGAGLFRLHNGVPQQFSMKNGLPSDNIRCLASSRNGDLWVCTENGLARFSKGTWFT
                     FRGAQGLSTNNFRAADEARDGSIWFGGDSANLSVWNGSAFTQHTLSSMPQYATVRALL
                     CSGDGSVWIGTSDGLIRLKNGVEHRFTAADGLAGNYIYCLREDRDGTLWIGTKNGFSR
                     WRNGEIESYRSKDGLSQSTVYSLMEDREGSLWAGTKNGLNQFLDGRAIPYTVSEGLPT
                     NSTGPVLQDRRGTIWVGTLGSGLARFDGRRFSVLTVKQGLASNTVYALAEDRNGDLWA
                     GTSAGVTRLHDGVAAQTYTTARGLPGNTVHALLRDHSGVLWIGTAHGLAVFQNGRVTI
                     PPSLRGHQFSVLALGEDGAGHMFVASANGDLWVYRNGKMEAFPGNATSPRNVDAFYTD
                     RDGYLWMGSLGGGLRLLKNGQVIRYFLRDGLFDNEIYGITADREDCLWMVCSKGVFSV
                     KRSDLLKFANGQIHTFASKPYSPLDGLRTIEGRGGVQPAAWKMQDGRLWFSTVRGLLA
                     LDASRVKHSLSPPPVVIEDVLVNGQSELPANIENLPPDRKNLEFHYTGLSFLLPSRIT
                     FRYILEGFDRKWIDAG"
BASE COUNT         1143 a         1781 c         1727 g         1259 t
ORIGIN      
        1 ggcggctcgg gatacaggca gcgatagcag ggaccgtcct catatgcaaa tacagtcgcc
       61 tgtccttcga agcggaaaat cgacccgtac acgttcggtt tccccagcag cacgcaggca
      121 tcgttcacca agtaccgcgt cggaaaattg tcggtcccgt cgatcacgat gtcgtaatct
      181 ttcaggattt cgagcgcatt ctcgctggtc agcgcggtct cgtgccggtc gatctgcacg
      241 tgcggattga tgtccagcat cgactcggcg gcagagtcga gctttttccg tccgacatct
      301 ttggtgccgt ggatcacttg ccgttggagg ttggtgaaat cgacggtatc gaaatccacc
      361 atgccaatac ggccaacgcc cgctgccgcc agatacaatc ccagaggcgc acccaatccc
      421 ccggcgccga tcagcagaac cttcgcctgc tttaacttca actggccgtc catcccgact
      481 tccgggatga tgaggtgccg gctgtagcgg agaatctctt ccttgctcag cgtgacgggc
      541 gctgcgacgg ggtcggcaac cgcggcggtt ccgcctgcga tactcggcac gatcgagacc
      601 gtgtcgccgt cctgcaggcc cgtcttgtct ttgtcgagga aacggatgtc ttcgtcgttc
      661 acatagacgt tgacgaagct ccgcagcctc ccttcgtcgg tgtacaagtg ccgcttcagc
      721 tcgccgtatc gcgaggtgag cgccgccagc acatcggcga ccgtcgcccc attcagatcc
      781 accgcgctct gcttctcggc atattgccgg aggggcgtcg gaattaacac cttagccata
      841 gatcaactct tccttttccg ccgcggtctg atcgagattc ggcaggaagc tgttcgcgtg
      901 atcgaatttg cctcccttga tcgacagaac cacgaatgaa taccacgggc atgaattttt
      961 caaatccgtc tcggagaaat acgcgtcgca atccggatgc gaatggaaaa taccgatcaa
     1021 gtccattcct tgttttcggg cttcgcgctc ggccgccagc agatcctcgg gccgcaactc
     1081 gtagcgcgcg ttctgcgcgc cttgataagc gttctcgatc ggcgaggcaa cgctgacctg
     1141 cttttcgacg ccgtccgaga cgccgagcat cgcgccgcaa cactcgtttg ggaacgtcga
     1201 ttcggcatgc gccaccatcg cgctccaagc tttcccttca atccggatca ttccaaaatg
     1261 gctcgcttaa atattttccg gcgccgtcgc acaggacggt cacaatcaca ccgctctcgc
     1321 cgcgttccac caaccggcgc gccacgctca tcgctcccac cagattcgcg ccactcgaaa
     1381 tgccaacgag gattccctcc tcgcgtccga gcttacgggt catcgcgtaa gcatcctcgg
     1441 tctcgatcca caagttatcg tcggcgaggt ccggatcgta gattcccggc acaatcgccg
     1501 tcggcatgtg tttcagaccc tccaggccat ggaacccgct ggagggttgc gcggaaatca
     1561 cccggatgcc cggcacctcc tggcgtagcc gccgcgatac gcccatgaac gtcccgctgg
     1621 ttcccatgca cgcgacaaag tgcgtaatac ggccttgggt ctgcgccacg atctcggggc
     1681 cggtcgtctc gtagtgcgcg cgccaattgg cgggattgtt gtactggtcg ggatagaaat
     1741 acgattccgg atcggctgcg tatacgtccc ggcacttccg aatcgccccg tccgagccct
     1801 ccgcgggatc ggtcaaaatg gtttccgcgc cgtaagcctt tagcatctgc ttgcgctcat
     1861 gcgaagcgtt ccgtggcaag cagaggcgca cgcggtaccc cttcgcggcc gcgatcattg
     1921 cgtaggccag cccggtgttt ccgctggtgg cgtccagaat gattttgtcg cgcgtgagtt
     1981 gcccgctgcg ctcaccttca aggatcatgt tcaacgccgc gcggtctttt accgacccgc
     2041 cggggttgaa atattcggct tttccgtaga tttcgatgcc tggaaggccg cgtgtaagct
     2101 tgtccaggcg cagcagcggc gtatttccga tctgacccag cagatttgtc gattcggttt
     2161 tcgatagggg cgcagccgct gtagccaatg gcatgaaggg ttactctatt gtaagctaaa
     2221 catcccgttc ccgctcaaga ttgggatgtt tatagtctca ggtgttagat gcaggattgg
     2281 gctgatcggt cacacggaga gttcgaccat gagcggcaat gcccgtctac aatctccggt
     2341 cgccggcggc gacaaatccg aaataggccg caatgaactg gtctttcggc aggtccacgt
     2401 gaatcgacga tcccgcagtg gtgggttcct tgtcgttgat tccccactgc cagacgcggc
     2461 aggtcacgga gttggctatt ctcaccgcgg atttgagcgt acagaagtgg ttcggcttga
     2521 gcgactgagc cgtgggatcc ttgaacatgt tgccattcac gcaaaacact acccggtaac
     2581 cctgactcca caattcgctg gcctgcttca ggttcttctc atccgctttc cagagaatcg
     2641 tggagtacgt ctcgttcagc acgttctggt atcccaggct cttgaagcag gatacgatct
     2701 caaacggcaa ggcgccctga atctgtatat tgaccgaggt gagagcgccg aagccaacgt
     2761 tcgcccggac gccagccatc gtcacccagt cgcacgcgcc gatgctggtg acctctgggt
     2821 caaggctcgt tgggatattt tggtcaagaa tgtggctgac gtcgaccttc cagccgcgca
     2881 ccgccgcttt cccagaaaag aacaaatcga gtgcgaacgt cgcgtactcc agaggactgt
     2941 cctttgccat tgcgtgaaga attgccgcag gtccgcagaa atctagatga ccctggttga
     3001 tgagtttggg gtcgttaacg cggtttctca gctcccgaat gaaatcgggc ttgctcaagt
     3061 gcgcgaacgc ctttttaccc atccctcgct gggtaatcag gtcctccgcc tcctcccgct
     3121 ccgccttttt ttgttcttta gtaagttttg cgagtgttgg ttgcattatg aggcgcccgg
     3181 catgtaggga agtatacacc ccggcctgcc catcccatta aggaccgggg cgattgatca
     3241 catccgcgag ttcaatctgg atcgtgcggt tcgcgagcgc gagttccagg atttccagga
     3301 tcacggccgc gaggatcagg cagctccccg ccacaaacag gacgaacgcg aaggaggtcc
     3361 aggagatgcg ccccggggcg aagacgatca gcagcaccat caccagaaag cacacgacag
     3421 ccgagtagag gcagcgctgc ccgttggcga cgtgggccat tcgccgctga aagagtccgg
     3481 cttgggccgc gatagtcctt tggcgccctt cgctcgcctg gcggaactcc acggcgagcg
     3541 tccgcacgcg gtccgacatc gattgatgct tgctgttgag accgaggatc aggacagccg
     3601 ccgccgagac catcactacc ggcgtaaccg cagccgccac ggtctgcggc aggcccgcca
     3661 tcgtgccagt caacatggct ttaattgtaa aagcgcgagc cgcccaaatg cagtctgggg
     3721 gagaatagtt ctaaggagcc ggttctgtcc cacttgaacg gcagcttgtc ctcgccgccg
     3781 cgaaacacca gttgccgtgt attaactcgt cgtgttttga ccggggcagc cattttctcg
     3841 ctcctggccc tttcgtcgtc ggggacggcg ctggggttga ttccggaccg cgcgctcacg
     3901 caatatcttc accgcatttg gcaggtaccg caaggtctac cacaggggac gatttactcg
     3961 attgctcaga cgcgcgatgg ttatctgtgg ctcggcacgc aaaccgggct ggtgcgcttt
     4021 gacggcgtgc atttcgcccc atttgaaagc agtggcggcg cggccccagg gaacgtctgg
     4081 gtacgatcgc tgctcgaaga cgggaatggc aacctctggg ccggcacgat cggggccgga
     4141 ttatttcgcc tccacaacgg cgtgccgcaa caattctcga tgaagaacgg gctgccgtcg
     4201 gacaacatcc gctgcctcgc atcgagccgg aatggcgatc tctgggtttg caccgaaaat
     4261 ggtctggccc gattctcgaa gggcacgtgg ttcacgttcc gcggggcgca agggctctcc
     4321 acgaataatt ttcgcgcagc cgatgaagct cgcgacggct ccatctggtt tggcggcgac
     4381 agcgccaatc tcagcgtgtg gaatgggtcc gccttcacgc aacatacgct ctcttcgatg
     4441 ccccaatacg cgaccgtgcg cgcgctgctg tgttccggcg acgggagtgt ctggatcgga
     4501 accagcgatg gattaatccg gctgaaaaac ggggtggagc accggttcac cgccgccgac
     4561 ggtctggccg gcaactatat ctattgcctg agagaggacc gcgacggcac gctgtggatc
     4621 ggcacgaaga acggcttcag ccggtggcgg aacggggaaa tcgagagtta ccgctctaaa
     4681 gacggcctat cccaaagcac cgtttattcc ttaatggagg atcgcgaagg aagcctctgg
     4741 gccggtacga agaacggcct gaatcaattt cttgacggtc gcgcgatccc ttacacggtc
     4801 agcgagggac ttcccacgaa cagcacggga ccggtattac aagaccgccg ggggactatc
     4861 tgggtgggga cgctgggatc gggtctcgcg cgcttcgacg gccgcaggtt ttccgttctg
     4921 accgttaagc agggccttgc gtccaatacc gtctatgcgc tggcggagga tcgcaatgga
     4981 gatctctggg ccgggacttc cgccggagtc acccgccttc acgatggcgt tgccgcccag
     5041 acctacacga cggcgcgggg attgcccggc aacacggtcc atgcgttgct acgcgatcat
     5101 tccggagtcc tctggattgg aactgcccac ggtctcgcgg tgtttcagaa cggcagggtc
     5161 acaattccgc caagccttcg cgggcaccag ttctctgttc tggcgcttgg agaagacggc
     5221 gccggccaca tgttcgtggc gtcggcaaac ggcgatctct gggtttaccg gaatggaaaa
     5281 atggaggcgt tcccaggcaa tgcgacgtcg ccgcgcaacg tggatgcatt ctacacggac
     5341 cgcgatggtt atctctggat gggctctctc ggcggcggct tgcggctgct aaaaaacggg
     5401 caagtgatcc gctacttcct tcgcgacggc ctcttcgata acgagattta cggtattacc
     5461 gccgatcgcg aagattgcct ctggatggtt tgcagcaagg gagtcttttc cgtaaaaaga
     5521 agcgatctgc tcaagtttgc gaatggccag atccatacct tcgcgagcaa gccgtatagc
     5581 ccgctcgatg gcttgcggac catcgaggga agagggggcg ttcagccggc ggcatggaaa
     5641 atgcaagatg gccgcctgtg gttttctacc gtccgggggc tgctggcgct cgatgccagc
     5701 cgcgtgaagc acagcctttc gccgccgccg gtagtgattg aagatgttct ggtgaacggc
     5761 cagagcgagc ttccggcgaa tattgaaaat ctgccgccgg atcgcaagaa cctggagttc
     5821 cactacaccg gcctcagttt tctgctgccc tcgcgaatta cattccgtta cattctcgaa
     5881 ggtttcgacc ggaaatggat cgacgccggc
//
LOCUS       DATNDT010000016         3733 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4013018, whole genome shotgun sequence.
ACCESSION   DATNDT010000016 DATNDT010000000
VERSION     DATNDT010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4013018"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1371)
                     /locus_tag="VN788_00640"
     CDS             complement(<1..1371)
                     /locus_tag="VN788_00640"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXU19068.1"
                     /translation="MKTLQSHLAILCAFLIAAPLLPAGETQTENGITIQAPTGGGPLG
                     GIIHPYQGHEVAPVNLTNSSRLDALIRAGKIYLSLQDTIALALENNLDIAVQRYGPLI
                     AEQDLKRAQSGGAIRGVQQSVQQGPSGGGQGVSSGQLGNPSANSGSSSGSTAGVNGII
                     TQLGPTTPNLDPVLQAFTGWFHQTSPQTSTFLTGTTSVVSKSQIYDFSLSQGFLTGGQ
                     AVLSYNDRVLNQNSPLNSVNPSTALSLDLTMTQHLLQGFGIAVNNRNIRVAQNEVKLS
                     DVVFREQLINTVSSIITLYWNLVSFNENVDFAKQNLALAQKTYDDNQKQVQIGTLAPI
                     EIVRAEAEVAARQQDLTIAETNVLQQETTLKNALSRNGIASPEVMEARIVPTDRIRVP
                     ENETVQPMPELVQLALRDRPEISQSKFNVENSRISLIGQKSGLLPTLDAVAELTNHGQ
                     SGDPNLK"
     gene            complement(1383..2876)
                     /locus_tag="VN788_00645"
     CDS             complement(1383..2876)
                     /locus_tag="VN788_00645"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXU19069.1"
                     /translation="MNASSSNAASSSGLSQLGPQIPTLDPVLKVSYTAEHFTSILTNL
                     RQAGTFSLVSSTKVPDVSISKNWLTGTSATLSLSNQFVLQNTGLNSLNPSRSATLDLV
                     VVQPLLQGFSIAVNNRDIRIAKNEVKLSNVVFKSQVIATVSSVVDLYWDLVAYNENVD
                     FAKQNLALAEKSYNDTKAQVQVGTLAQIETVRAEAEIASREQDLTVAQTSVLEQETIL
                     KNALSKNGVADPLVAEARIVPTDRIRISDTDGTEPLPNLIDKALQNRPELEQSRLGVE
                     NNQIQLKGTKNELLPTLDLFVELTNHGLAGVVNAVPLPPGFEPLPPANPYFLGGYGTA
                     LGQIFRRNFPDYTIGFQLNIPFRNRASQSDYVHDQLTLRESQFNYRQQENQIRVDVQN
                     AVVALRQARAQYDAAIKQRVLEEQTLDAEQKMNALGAATVFEVIQAQRDLAQAQSSQV
                     SAESQYQKARVNLEQVTGRTLSAYNININEAISGRVSRPPSPIQANP"
     gene            2857..3180
                     /locus_tag="VN788_00650"
     CDS             2857..3180
                     /locus_tag="VN788_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19070.1"
                     /translation="MLDEAFTDDAIDEDAALVDEGLLEKAELLPLGEDDEVPVEDAPP
                     AADAADVVVPVVTVDAVPAVPAAPAVPVVVDVVAAGVAGVPVAGGVDVEVAEIVGAGA
                     LAGPC"
BASE COUNT          736 a          969 c         1192 g          836 t
ORIGIN      
        1 cttcagattg ggatcgccgg actggccgtg gttggtgagc tcggcgacgg catccagagt
       61 cggcaaaagg ccgctttttt ggccgatcaa gctgatgcgg ctgttctcga cattgaactt
      121 gctctgggaa atctcaggcc ggtcccgtaa cgcgagttgc acgagttctg gcatcggttg
      181 gaccgtctcg ttctcgggaa cgcgaatgcg atcggtcgga acaatgcgcg cctccatcac
      241 ttcgggactg gcgattccgt ttctgcttaa ggcgtttttg agagtcgttt cctgctgcag
      301 gacgttagtt tccgctatgg tcaaatcctg ctggcgggcc gcgacttcgg cttcggcgcg
      361 cacgatctcg atcggtgcaa gagtgccaat ttggacctgc ttctgattgt cgtcataggt
      421 cttctgggca agggcgagat tctgtttggc aaaatcgacg ttctcgttga aactgaccag
      481 gttccagtac agcgtaatga tgctggatac ggtgttgatc aactgctcgc ggaagactac
      541 gtccgacagt ttgacttcat tctgggccac ccggatgttg cggttattta cggcaattcc
      601 aaaaccctgt aggagatgtt gcgtcatggt aaggtcgagc gacagcgcgg tggatggatt
      661 tacggaattg aggggggagt tttggtttaa aacgcgatcg ttgtacgaaa gaacggcctg
      721 gccgccagtg agaaagcctt gggagagcga gaaatcgtag atctggctct tgctgacgac
      781 tgatgtcgtg ccagtgagaa aggtgctggt ttgagggctg gtctgatgga accagcctgt
      841 gaaagcctgg agcacggggt cgaggttcgg ggtcgtggga ccaagctgag tgatgatgcc
      901 gttcacgccg gcggtcgagc cgctgctgct tccgctgttg gcgctcggat taccgagctg
      961 cccggaacta acgccctgcc cgccgccaga cggaccctgc tggacggact gctgcacgcc
     1021 gcggatagcg ccgccgctct gcgcgcgttt tagatcttgc tcggcgatca gtggaccata
     1081 acgctgcacg gcgatatcaa gattattctc gagcgcgagc gcgatggtgt cctgcagcga
     1141 gagatagatc ttcccggcgc ggatgagcgc gtcgaggcgg ctcgagttcg tgaggttgac
     1201 cggcgctacc tcatggccct gataagggtg aatgattccg ccgagcggac ctccgcccgt
     1261 gggcgcctga atggtaatcc cgttttcggt ttgcgtctcc ccggcgggca gaaggggcgc
     1321 cgcgattaga aacgcgcaaa gaatggccag atgggattgc aaggttttca tacgatactg
     1381 tcctatggat tcgcctgtat cggcgagggc ggacgcgaaa cgcggccgct gatcgcctca
     1441 ttgatattga tgttgtaggc ggagagggtg cgtccggtta cctgctccag attgacgcgc
     1501 gccttttgat attgactctc cgcgctgact tggctggatt gagcttgcgc caaatcgcgc
     1561 tgcgcctgaa tcacctcgaa aaccgtggcg gcgccgagcg cgttcatttt ctgctcggca
     1621 tcgagcgtct gttcttcgag gacacgttgc ttgatcgccg cgtcgtactg cgcgcgagcc
     1681 tgccgcaacg cgaccaccgc attctgcacg tccacgcgga tctggttctc ctgctgcctg
     1741 tagttgaatt gggattcgcg caacgttagc tgatcgtgga cgtagtccga ttgcgacgcg
     1801 cggttgcgaa agggaatatt caactgaaag ccgatggtgt aatcgggaaa gttccgccgg
     1861 aaaatctggc ccagcgccgt gccataacca ccgagaaagt atggattcgc gggtggcagc
     1921 ggttcgaagc cgggcggcag cggaacggca ttcaccacac ccgcaaggcc gtggttggtc
     1981 agttccacga aaaggtcgag cgttggaaga agttcgttct ttgtgccttt gagttggatc
     2041 tggttgtttt caaccccaag gcggctctgt tcaagttcag ggcggttctg aagcgcctta
     2101 tcgataaggt tgggcagcgg ctcagtcccg tcagtatccg agatacgaat ccggtctgtc
     2161 ggaacgatac gggcttccgc gacgagcggg tcggcgacgc cgttcttgct gagcgcgttt
     2221 ttcagaatcg tctcctgctc caaaacgctc gtttgagcta ccgtgagatc ctgctcgcgc
     2281 gaggcgattt cggcttcggc gcgcactgtc tcaatctggg cgagcgtacc gacctgtacc
     2341 tgcgccttcg tatcgttgta gctcttctcg gcaagagcga ggttctgttt cgcgaaatcg
     2401 acgttttcgt tataggccac gagatcccaa tagaggtcca cgacgctcga cacggtggcg
     2461 attacctgcg atttgaagac gacattagag agtttcactt cattcttagc aatgcggatg
     2521 tcgcgattgt tcaccgcgat gctgaacccc tggaggagcg gctgaaccac gacgagatcg
     2581 agggtggccg agcgggaagg attcaggcta ttcagtccgg tattttgcag cacgaactga
     2641 ttgctcaacg agagcgtggc gctggttccg gttaaccaat ttttcgagat actgacatcc
     2701 ggaacctttg tcgaagaaac gagcgagaaa gtacctgcct ggcgcagatt cgtgagtatg
     2761 gaagtgaaat gctccgcggt gtagctgact ttcagcaccg ggtcgagcgt cggaatctgc
     2821 ggtccgagct gcgaaaggcc gctgctcgat gcggcgttgc tcgatgaagc gttcacggac
     2881 gatgcgatcg atgaagacgc tgcgctggtg gatgaaggat tacttgaaaa agccgaattg
     2941 ctcccgctgg gagaggacga tgaagtaccc gtggaggatg cgcccccggc ggccgatgcc
     3001 gccgatgtag tggttcccgt tgtgaccgtc gacgcggtgc ccgcagttcc ggcggcgccg
     3061 gctgttccgg tggtggtcga tgttgtggcg gccggagtcg ctggcgtccc cgttgcgggt
     3121 ggagtcgacg tcgaggtggc ggaaatcgtc ggcgctggcg cgctcgcggg cccttgttga
     3181 atcgtctgcg agataccacg gttcaggccg ccgcttttcg cgcgcttcaa atcctgttcc
     3241 gcgatcagcg gcccgtagcg ttgagccgcg atgtcgagat tgttttcaag cgctagagcg
     3301 atggcgtcct gaagcgagag atagattttc ccggcgcgga ggagcgactc aagacggccg
     3361 gagtttgaaa gattgacggg tgaaatggaa ggcgcgcgat aactacggaa caggccgtgc
     3421 ggaggctgga tggcgattcc accaaacgat tgctgagccg gagcgagtgg tacaaaaaaa
     3481 agacacacgc ataacactgc cactacaggc ctaaaaatat tcatgtccta cccaggtaac
     3541 ggttgcagaa gctcctagct ttcgataacg caaaaaagag cgaggctgtt cccaaaaact
     3601 tcaatcgtag cacgggcaat ctcattgcca ctgtggaact tgcgcaaata gctgtgaacg
     3661 ttcggtgtgc gcacggcgaa ttccgaggga tgtgtgcgaa tatgggacgc ctgcgctgaa
     3721 ggcggacagc cgc
//
LOCUS       DATNDT010000017         5474 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2851481, whole genome shotgun sequence.
ACCESSION   DATNDT010000017 DATNDT010000000
VERSION     DATNDT010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2851481"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2466)
                     /locus_tag="VN788_00655"
     CDS             complement(<1..2466)
                     /locus_tag="VN788_00655"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU19071.1"
                     /translation="MARPILNRLRRLGNSFCAAALLALAISAPKVYGQAAATGAILGT
                     VTDPSGAAIPNAQIEITNMATQEKRTLSTNAAGLYDAEGLQASGTTYSVVITQQGFKS
                     FQSKGVKLDAGTRVTLNARLELGDTSSQVTVEASPLAVQTESGVTGGVVSSTQISQLQ
                     LNGRNFLTLQILVPGVNPTDSAQEQGGGGLTTFNHISVNGMGEEFNQVMVDGIYNMNT
                     GDESQLNFNPPLDSIAEVRVLTGSYSARYGLTGAGISLVETKSGTNQFHGSAYEFFRN
                     DAMDSRPFFATSISPLKQNIFGFSIGGPVWIPGRSKSNTKTFFFGNEEWRRRHSGYVF
                     RTALPTSAILGGNFSADPTLKGGALQLDQSSQQLLAAAHPGVNCITNSTRVNPACFDQ
                     NSLAMINKFWPLPNVSSGFLNYNTTPVDVVSQRDDLWRVDHQFNEKYSLMVRYSREVV
                     QDNSPTASPNIGWTQPGGDPGIGDNILTTSFNNMVRFNMNMTPHIINSMTFGQTSDKP
                     RLHPVGASPPDGLTINFPYPNADPFGRTPLVSVAGGWASMGTNSEPVNASDGELTYSD
                     DFTLVVGRHVLQAGVLYINGVKRQDADNQVMGAYGFSGSHTGSPMADFLLGLDSSFSQ
                     SNLQRRAYIHYWQLEEYFQDDWKVTPRFTLNLGLRNLYFSPDTFEGNGLSDFDPSKFD
                     RSMAPAVQPNGQFVFNAAGQPLTPSGTVANPLTGLVFRGQGGVSAGVYRVPTFNLQPR
                     FGFAYDLTGNGKTVIRGGGSIGYNRIPLNAVLGTVSNPPFVTTSTFLNGTVTNPAAGS
                     QTLITPRSLSIVGPPDELFKPARI"
     gene            2647..4944
                     /locus_tag="VN788_00660"
     CDS             2647..4944
                     /locus_tag="VN788_00660"
                     /inference="COORDINATES: protein motif:HMM:NF039707.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6259 domain-containing protein"
                     /protein_id="HXU19072.1"
                     /translation="MIRTYRRLTLFIAALYLFFALSGNAQINSGHENQISLKSNEIEL
                     ALDPSSGYSIVGLRDPKTGTDFAATGARPGLYRIVMSRSDGSSYEITSKSAKCVRSSA
                     PDRITLSFDHPEQGLRVIGTVRLDAAQKLGWTLRIENHGNDGVRSVFYPQWIAPPRLS
                     QSALENRILYPSMDGQEFVDPAKHFTPGQTVSSGYPGLAAVQMIAYHDDSAGLLEMTR
                     DGNGENKHFRIVRRPDGFDLSIEHNPGETPGTNIDLPYETSMQVFHGDWHEAADIYKT
                     WAVQQKWAARKIRERGTPRWLTSGYPIVTYQMRGDPYSAEWSMYFPPSARLLNPDFYP
                     TKIPAIAKSYENFFQSKVISNPFGWEHNGPWIAGDYFPPVLGEETWRDIAAKLHAEDS
                     PLFLLLSGLRWGVRMDDLGYNTREQFLRDVAPKAAAYDPQGKPIEDNAPWGDSIQLCV
                     GTNFGRESTAVAFLGCIRRGASLVQYDQSAGGTAPVCYSHVHPHPPGYGRWMVQGTES
                     LFTEVRAKGKRINPEFALSVEEPCEYFIPYWDLYMGRPYEFFGTGSDPSSWRTSVPLF
                     LYIYHEYLLGYGGSNEIDVTHPYAEAIKVARKFTNGTLLEIDPGKPAFRLDTQPSPTE
                     ELRLARSCSLAQRTYANDYLIFGKMLRDPRIPDRKTQTLRAWHDPRDRHPDSELPTVQ
                     VPLVLESTWESSNKVAYVFANWQTSAQEVVFEPQRYDQNGAGFKLTVYDDSGTRVLRR
                     DPSLPREIRLRIPALAATMVEQSAE"
     gene            4953..>5474
                     /locus_tag="VN788_00665"
     CDS             4953..>5474
                     /locus_tag="VN788_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19073.1"
                     /translation="MTRKTFLESTLLLGTKPGRALIGGGVALAQSPTTADGVFALKNS
                     NHALEFSRSTGQLLSFEAEGQQFVSAAPGDPVFVIQYLDERDEFEHVDSTKSKSVHVR
                     LEEPNGKGFVLTATFDGVGGLDLDATVTVRQGAEDPFSRWSIAISNRSGIRITDVQFP
                     FVVVRYNLGGGAGS"
BASE COUNT         1194 a         1464 c         1580 g         1236 t
ORIGIN      
        1 aatcctggcc ggtttgaata gctcgtccgg aggaccgacg atactcagtg agcgcggagt
       61 gatcaaagtc tgggaaccgg cggccggatt ggttacggtc ccgttcagga acgtggaagt
      121 agtgacgaaa gggggattcg aaacggtgcc cagcactgca ttcaacggaa tgcggttata
      181 accgatggag cctccgccgc ggataaccgt tttcccgttg ccggtcagat cgtacgcgaa
      241 gccaaaacgc ggctgaaggt tgaatgtggg aactctatac acgccggcgg aaacgccgcc
      301 ctggccgcga aatactaatc cagtcagcgg atttgcgact gttccagagg gcgtcaatgg
      361 ttggccggcc gcattaaaca caaattggcc gtttggctga acggcaggcg ccatgctccg
      421 atcgaatttg gacgggtcga agtctgaaag cccatttcct tcgaaagtat cgggcgaaaa
      481 atagagattg cgcaaaccga ggttcaatgt gaagcgcggc gtcaccttcc agtcatcctg
      541 gaagtactct tcaagctgcc agtagtgaat ataggcccgg cgctgcaagt tgctttggct
      601 gaaagaactg tccaggccga gaaggaagtc ggccatcgga gaaccggtat gagatccgct
      661 gaaaccataa gcgcccatta cttgattgtc ggcatcctgc cgcttgacac cgttgatata
      721 aaggactccg gcctgcaaca catgacggcc cacaaccagc gtgaaatcgt ctgaataagt
      781 gagttcgcca tcgctggcgt tcacgggctc gctattcgtc cccattgacg cccaccctcc
      841 cgcgacgctg acgagcggcg tccggccaaa gggatcggca ttgggatagg ggaaattaat
      901 cgtgagaccg tcgggaggag acgcacccac cgggtgcagc ctgggtttgt cagaggtttg
      961 gccgaaagtc atcgagttga tgatgtgcgg cgtcatgttc atgttgaacc gcaccatgtt
     1021 gttgaagctg gtggtgagaa tattgtcgcc gattccagga tctccgcccg gctgcgtcca
     1081 accgatattc ggggacgccg tcggagaatt gtcctgcact acctcgcggc tatagcgcac
     1141 catcaaacta tatttctcgt tgaactggtg atccacgcgc cagaggtcat cgcgctggct
     1201 tacaacatcc acgggagtcg tgttgtaatt caggaatccg gagctcacgt tcggcagcgg
     1261 ccagaattta ttgatcatcg ctagggaatt ttgatcgaag caggccgggt tgacgcgcgt
     1321 tgaatttgtg atgcaattca cgccgggatg tgccgctgcc aggagctgtt gcgacgattg
     1381 atccaattgc aacgcgccgc ccttgagggt tggatcggcg ctgaaattgc cgccgaggat
     1441 tgccgatgtc ggcaaagccg tacggaagac gtagccggag tgacgccgac gccattcctc
     1501 atttccaaag aaaaacgtct ttgtgttgct tttacttctg ccaggaatcc aaacggggcc
     1561 cccgatactg aagccaaaga tattctgctt gagcggcgat atgctggtcg cgaaaaatgg
     1621 acgcgaatcc atcgcatcgt tccggaaaaa ttcgtaggcc gagccgtgga actgatttgt
     1681 gccggacttc gtttcgacga gggagatgcc cgctccggtc agtccatacc tggcgctgta
     1741 gctaccagtg agtacgcgga cttctgcgat tgaatcgagc ggcggattga aattcaactg
     1801 cgattcatcg ccggtgttca tgttgtagat gccatccacc atcacctggt tgaactcttc
     1861 gcccatgccg ttcaccgaga tgtgattgaa ggtcgtgagg ccgccaccgc cttgttcctg
     1921 ggcgctgtcg gtgggattga ctccaggaac cagaatttga agggtcagga agttccgtcc
     1981 gttcagttga agctggctga tctgcgttga actgacaacg cccccggtga cgccgctctc
     2041 ggtctgcacc gcaagcggag atgcctccac agtcacttgg gaggaagtat cgccaagttc
     2101 cagccgggcg ttcagcgtaa cgcgggtccc ggcgtcgagc ttcacgcctt tggactggaa
     2161 cgacttgaaa ccctgttgcg tgataaccac tgagtaggtt gtgcccgatg cctgaaggcc
     2221 ctctgcatcg tagagaccag ccgcgttggt ggataacgtc cgtttctcct gagtcgccat
     2281 attcgtgatt tcaatttgcg cgtttggaat cgcggccccg ctgggatccg ttaccgttcc
     2341 aagaatggct ccagtcgcgg cagcttggcc gtaaaccttc ggagcgctaa tcgcgagggc
     2401 gaggagggca gctgcgcaga aactgttgcc aagccgtctc agccggttta gaattggtct
     2461 ggccatcaga tgaactccgg attcgaaact atcattaacg tgtttttctg tcaatggcga
     2521 gatataagaa atgtcgtttt actttgaatc taagcgcatt aattgatttg atatgtccga
     2581 ttcgcaactt tgtaaccaaa gccacgttgg agaatcattc ggtgcggcgc aaggaaactt
     2641 tcgcaagtga ttagaacata tcggcgttta accctattca ttgccgcgct ttacctcttt
     2701 tttgctcttt ccggtaatgc gcaaataaac tccgggcacg agaaccaaat aagtcttaaa
     2761 tcgaatgaaa tcgaactcgc gctcgatcct tcgagtgggt acagtatcgt tggattgcga
     2821 gatccgaaga ccggcacgga tttcgccgcg accggcgcaa ggccggggtt gtaccgaatt
     2881 gtgatgagcc gctcggacgg ctccagctat gagatcacgt cgaaatcggc gaaatgcgtc
     2941 cgctcttcgg cgcccgatcg catcactttg agctttgatc atcccgagca aggccttcgc
     3001 gtgatcggca cggtgcgcct ggatgcggcg cagaagcttg gttggacatt gcggatcgag
     3061 aaccacggta atgacggggt tcgttccgtg ttttacccgc agtggatcgc tcctccgcgt
     3121 ctctctcaat cggcgctgga gaatcggatt ctttatcctt ccatggatgg ccaggagttc
     3181 gtcgatccgg cgaagcactt cactccgggc cagactgtca gctcgggata tccggggctt
     3241 gccgccgttc aaatgatcgc gtaccacgac gattcagccg gcctgttgga gatgactcgc
     3301 gatggtaatg gggagaacaa gcattttcgg atcgtgcgcc gcccggatgg attcgatctg
     3361 tcgattgagc acaatccagg cgagactccg ggaacaaata tcgacctgcc gtatgagact
     3421 tcgatgcagg tgtttcatgg cgattggcat gaagctgccg acatttacaa aacatgggcc
     3481 gttcagcaga aatgggcagc gcgaaagatt cgcgaacgtg ggacgcctcg ctggcttact
     3541 tcgggttatc cgattgtcac ttatcagatg cgcggggatc cttactcggc ggaatggtcg
     3601 atgtactttc ccccgtcggc gcgcctatta aatccagact tttatccgac taagattccg
     3661 gctatcgcga aatcttacga aaacttcttc cagtcaaaag taatcagcaa cccgtttgga
     3721 tgggagcaca acggcccctg gatcgctggc gactacttcc cgccagttct gggcgaagag
     3781 acttggcgcg atattgcggc taagctccat gcggaagact caccgctttt tctactgttg
     3841 tccgggctgc gatggggtgt acggatggat gacctcgggt ataacacgcg cgagcagttt
     3901 ttgcgcgatg tggcccccaa agcagccgcg tatgatccgc aaggtaaacc gatcgaggac
     3961 aatgcaccgt ggggcgacag tattcagctt tgcgtgggaa ccaatttcgg aagggaaagc
     4021 accgccgtgg ctttcttggg ctgtatacgc cggggtgcgt ctctggtgca atatgatcaa
     4081 agcgccggtg gaactgctcc ggtgtgctac agccacgtcc atccgcatcc gccgggatat
     4141 ggacgctgga tggtgcaggg aacggagtcg ctcttcaccg aagtccgtgc gaaagggaag
     4201 cgcatcaatc cggagttcgc actgtcggtc gaggagccat gcgaatactt catcccttat
     4261 tgggacttgt acatggggcg tccgtacgaa ttcttcggta ccggctccga cccgagttcc
     4321 tggcgcacaa gcgttccgct gttcctgtat atctatcatg agtatctgct cggatacggc
     4381 ggcagcaatg aaatcgacgt aacgcacccc tacgccgagg cgatcaaagt ggctaggaaa
     4441 ttcaccaatg gcactctgct cgagatcgat ccgggcaagc ctgcttttcg cctcgacaca
     4501 cagccgtctc ctacggaaga actccgcttg gcgcgcagtt gcagtctggc gcaaagaacc
     4561 tatgcgaacg actatctgat cttcggtaag atgcttcgcg atccccgcat tcccgatcgc
     4621 aaaactcaga cccttcgggc gtggcatgat ccgcgcgata gacatcccga ttccgagctt
     4681 cccacggttc aggttccgct ggtgcttgag tcgacgtggg aatccagcaa taaggtcgcc
     4741 tatgtgttcg ccaattggca gaccagcgcg caggaagttg tttttgagcc tcaacgatat
     4801 gaccagaacg gagccggttt caagctgacg gtttatgacg attccgggac tcgagtgctg
     4861 cggcgcgatc cgagcctacc acgcgagatc cggctccgaa ttcccgcttt agccgcgacc
     4921 atggtggaac aatctgcgga gtgagtttat aaatgaccag aaaaaccttt ttagaatcga
     4981 ctttacttct cggaaccaaa ccgggccggg cgcttattgg aggtggggtc gcgctcgcac
     5041 aatccccaac gactgccgac ggcgttttcg cccttaaaaa ctcgaatcac gcgctcgagt
     5101 tcagcaggag tacgggccag ctattgagct ttgaggcgga gggccagcag tttgtcagcg
     5161 ccgctccggg tgatccggta ttcgtgatcc agtatttgga cgagcgcgat gaattcgagc
     5221 atgtagactc cacgaaaagc aagtccgttc atgtccggct ggaggaacct aacggaaaag
     5281 ggttcgttct caccgcaacg tttgacggtg tcggcggcct agacttggac gccacggtta
     5341 cggtgcgcca aggggcagaa gatccattca gccgctggtc gatagcgatc tcgaaccgct
     5401 ctggaatacg cattacggac gtgcagtttc cttttgtcgt ggtccgctac aaccttggcg
     5461 ggggcgcagg ttcg
//
LOCUS       DATNDT010000018         6122 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2538976, whole genome shotgun sequence.
ACCESSION   DATNDT010000018 DATNDT010000000
VERSION     DATNDT010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6122
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2538976"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..757)
                     /locus_tag="VN788_00670"
     CDS             complement(53..757)
                     /locus_tag="VN788_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU19074.1"
                     /translation="MQSTGKIAIRTDHLTRHYQMGAALVRAAQDVTLDIAAGDFVAVL
                     GSSGSGKSTLLNLIAGLDRPTSGAILAHGRNLAEMSSEELAKYRSHTVGIVFQAFNLL
                     PRMTLEENVELPLRLAEVDRNERTARVREAVERVRLGARLSHRPTELSGGEQQRAALA
                     RALVNRPTILLADEPTGNLDSATGDEIMLLLREINETLGVTIVMVTHERPRAERFAHR
                     MIQMSDGKVVSDGAPQ"
     gene            complement(863..1267)
                     /locus_tag="VN788_00675"
     CDS             complement(863..1267)
                     /locus_tag="VN788_00675"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU19075.1"
                     /translation="MGLRILVVDDEQAIRTALQRLLSSHEGWAVVGTASDGVEAIAKA
                     KELKPDVIVMDISMPQMNGLSATPQIKRAVPDAEVLIFSQYAIHGMIQQAEEAGASGY
                     LLKSQTRSIIPAIEALAEHKFFFDADALTDAP"
     gene            1542..1772
                     /locus_tag="VN788_00680"
     CDS             1542..1772
                     /locus_tag="VN788_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19076.1"
                     /translation="MKPTYDIFRRLPDGGPIWIEAVQSLQFAKDRLTSLLEAQPGEYF
                     VYDLFQSKIVEYLDSRSLREDESALLQPPHQK"
     gene            complement(1749..1904)
                     /locus_tag="VN788_00685"
     CDS             complement(1749..1904)
                     /locus_tag="VN788_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012285225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HXU19077.1"
                     /translation="MLLLILIIVLLLALFGSLPTWPHSRNWGYYPSGGISLIVLILLI
                     LFLMGRL"
     gene            2079..2729
                     /locus_tag="VN788_00690"
     CDS             2079..2729
                     /locus_tag="VN788_00690"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXU19078.1"
                     /translation="MMVKPGLSSLILLTGRSLRHTLVQLGGVGLIPLGLLDSSLLPLP
                     GSMDIATIFLAARDTHLWLYYALMATAGSVAGGFITYRLARKGGKEALEHRSARGYMH
                     KVCEIFEKWGFAAVAIPALLPPPAPVTPFVVAAGAMQYSLKKFLAAFTSARLVRYVVL
                     ASLAAHYGHQIIDLISHNLLLVSIVIALLLGAAVALAFIVRGGVARRRAARKAKKK"
     gene            complement(2745..3233)
                     /locus_tag="VN788_00695"
     CDS             complement(2745..3233)
                     /locus_tag="VN788_00695"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXU19079.1"
                     /translation="MGLLKFCEWLSHTSWSVSLREGTYDYPVLLIIHVLSIALFGGMV
                     AIGNLRVLGAAMRRVPVSQVIEQFRPWKWIGFGVLLATGVLLSISDPVEYGANVMYWI
                     SLLLLVAAGVNAFVFRFGIYRSVASWDDAPITPPSARRWAGVSLALWIALIFAGRAIA
                     FF"
     gene            complement(3274..3762)
                     /locus_tag="VN788_00700"
     CDS             complement(3274..3762)
                     /locus_tag="VN788_00700"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXU19080.1"
                     /translation="MWAAVFVLHLLQGIPEALPLNPIARWEWVFPVVEAIHICGFSLL
                     VGTILILDLRLLGWCLPRQPISVIAKSLAPWIWIGIAIQLTTGPYLFSSDPHEYVQVA
                     AFRNKMLFLALALIFHFTVIRRATQPSRDAASPLWRKPAACVSLGLWLCVVLGGLWIG
                     NL"
     gene            complement(3762..4256)
                     /locus_tag="VN788_00705"
     CDS             complement(3762..4256)
                     /locus_tag="VN788_00705"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXU19081.1"
                     /translation="MTWIKVILLSFCHWCNETWFGHGIRDSVWLFPFVEIFHLLALGV
                     LGGTILLVNARLLGLRFADEPTSELAQDVWPWTLGSLAVMLISGFLLFSTEAVKMYGN
                     WGFRFKMTSLLLAIIFTFTIHRRITLSDEGRVHPIWCKVAAVVSLLLWAGVGLGGRSI
                     GYIG"
     gene            complement(4272..4772)
                     /locus_tag="VN788_00710"
     CDS             complement(4272..4772)
                     /locus_tag="VN788_00710"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXU19082.1"
                     /translation="MWFASFLQWLSDTQFSMSMRDSLWAEPIVETIHVLTLTLFLGFA
                     VLLDLRLLGVSMRRRPVSEVLRQLNPWLIAGFVVMIISGVLLFCGDPVSFYSTTFFKV
                     KMIMLVLAGLNVLIFNATIGRRTAEWDRAVRTPRAAKIAAVVSLVLWVAIVAAGRGIA
                     YALPPP"
     gene            complement(4830..5642)
                     /locus_tag="VN788_00715"
     CDS             complement(4830..5642)
                     /locus_tag="VN788_00715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19083.1"
                     /translation="MKRWNGWSGIAALFSILGVLAFAAPARAQFGPNAGPPPPKLNPH
                     TDAPFDPSGYWVAVVTEDWRYRMVTPDKGDFPGVPVNAEGRKIAEEWDPAKDQASGNA
                     CKAYGAAAIMRVPERLHISWVNDDTLRVETDAGEQTRIFHFDETKPPSGPPTWQGYSV
                     AGWEGMRPRGRVVPVAQGTPAAAKPKEEGYMKVMTTHVRPGYIRRNGVPYSANTSVEE
                     YFDSFEERGITWLVVTTIVHDPEYLAQPYITSSQFKKQADNAGWNPTPCEAK"
     gene            complement(5708..6097)
                     /locus_tag="VN788_00720"
     CDS             complement(5708..6097)
                     /locus_tag="VN788_00720"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXU19084.1"
                     /translation="MKRAVFILLAVAAALVMGGRAYAHHSFAATYFVDQEITVEGTLT
                     QFMYRNPHSFVKVEAPDSKGQMQTWSVEWGGGAQLTRDRVSRDTLKPGDHVVVTGNPG
                     RDPAEHRIRLHKIVRPSDGWKWEGVIM"
BASE COUNT         1340 a         1872 c         1641 g         1269 t
ORIGIN      
        1 agcagagctt cgcgcagatt gcggcccgca agctcagcga gatcgccgat tttcattgcg
       61 gcgctccgtc gctgaccact ttgccgtcgc tcatctgaat catgcgatgc gcgaagcgct
      121 cggcgcgcgg gcgttcgtgg gtgaccatca cgatggtcac tcccagcgtt tcattgatct
      181 cgcgcagcag cagcatgatt tcgtctcccg tggcgctatc gagattgccg gtaggctcgt
      241 cggccaacag gatcgtgggt ctattcacga gggcgcgagc caaagcggcg cgttgctgct
      301 cgcctccgga aagttccgtg ggacgatgac ttaggcgcgc tccgaggcgc acgcgttcga
      361 ccgcttcgcg cacgcgggcg gtgcgctcgt tgcgatccac ttcagccaag cgcagaggca
      421 gctcgacatt ttcttccaga gtcattcgcg gaagcagatt gaaggcttga aacacaatcc
      481 caacggtgtg actgcgatac ttggccaatt cttcggaact catctccgct aaattgcggc
      541 cgtgcgcgag aatcgctccg gaagtgggac gatcaaggcc cgcgatcaag ttgagcagcg
      601 tggattttcc ggagccggag ctccccagca ccgccacgaa gtcgcccgca gcgatatcga
      661 gagttacatc ctgcgccgcg cgaaccagcg cagcgcccat ctgatagtgc cttgtaaggt
      721 gatcggtgcg gatggctatt ttgccggtcg attgcatcgt ccgccttcca tgttactacg
      781 cgcgaaggtc gaacgatgtt gcggagatga cgagcggtgc gagaacgcgg cgaaatcggc
      841 ccccttattt tcaagccgcg gatcagggag cgtctgtcag cgcatccgcg tcgaagaaaa
      901 atttgtgctc agccaacgct tcgatagctg gaatgatcga gcgggtctgc gacttgagca
      961 aatatccgct ggcgccggcc tcctccgcct gttggatcat tccgtggatc gcgtattggc
     1021 taaagatgag tacttcagcg tcgggcaccg ctctcttgat ctgcggcgtg gcgcttagtc
     1081 cattcatctg cggcatgctg atgtccatta cgatcacgtc cggcttgagt tctttggcct
     1141 tggcgatggc ttcaacgcca tcgctggccg tgcctacaac ggcccagccc tcatgagaac
     1201 tcagcaggcg ctggagggcg gtgcgaatgg cttgttcatc atcaaccacc agtattcgta
     1261 atcccaccac gggcaccttt cgccggctgg cctgcctttt tcgcgcgact atcgccggtc
     1321 ggctgcggcg gttctaagcc gtcagagaaa tatctaccgc agcgctgcac gaactacacg
     1381 cttggcgggc tgagaagccc cagccaaatg tggggcgaaa gtcggcctaa gcatctggct
     1441 ctattactct taattgcaat tggatgcggc tccttccctg gcaatgcaat tgcacccact
     1501 caggtattgt ttccttcgtg aatccactct gaggagcacc cgtgaagcct acgtacgata
     1561 tttttcgaag acttcccgac ggcggtccca tctggatcga ggctgtgcaa agcctccaat
     1621 tcgcgaaaga tcgcctgacg tcgttgctcg aggcccagcc gggcgagtac tttgtgtacg
     1681 atctctttca gagcaagatc gttgaatacc tggatagccg cagcttgcgt gaagacgagt
     1741 cagctctcct acagccgccc catcagaaat aaaatcagta atatcaaaac gattaggctg
     1801 atccctccgc tcgggtagta tccccagttc ctgctgtgcg gccacgtagg caagctgccg
     1861 aacagcgcca gcagcaatac aataatcagg ataagtagga gcatgtgccc attctccttt
     1921 ttattgaccc gctaaccgcg ccttcgcgac cccgaaattg ccgccttttc ctcgtgtaga
     1981 ctactgggtg cgccattgca ccggcaatcg agcaaagcct gtagttactt gggctagggt
     2041 cggtagcatc ggcttacagt gcgagtacac ttgaactgat gatggttaaa cccggcctgt
     2101 catcattaat cctattgacg gggcgcagct tgcgccacac gcttgtccag ttgggcggcg
     2161 tgggcttgat tcccctcgga cttctcgata gttccctcct tccgcttcct ggcagcatgg
     2221 acattgcaac gatttttttg gcggcgcgtg atactcacct gtggctgtat tacgccctca
     2281 tggcaacggc aggttccgtt gcaggcggat ttattaccta ccggctggct cgcaagggag
     2341 gcaaggaagc gctggagcac agatccgcac gcggatacat gcacaaggtt tgcgaaattt
     2401 tcgaaaagtg ggggtttgct gcggtggcca tccccgccct gttgcctccg ccggcgcccg
     2461 ttacgccatt cgtggtagcg gcgggagcca tgcagtattc gctgaagaaa ttcctggctg
     2521 ccttcacctc agcgcgtctc gtacgatacg tcgttctggc gtccttggca gcccattatg
     2581 gacatcaaat tatcgatctg atctcacaca atttgttgct tgtgagcatc gtgatcgctc
     2641 tcttgttggg agcggccgtg gctctggcgt tcatagtccg gggtggagtc gcgcggaggc
     2701 gggcggcgag aaaagccaaa aagaaatgac aagtccgcga tcagttagaa aaaagctatg
     2761 gctcgaccag cgaatatcag cgcaatccaa agagctaacg atactcccgc ccaccgcctt
     2821 gcgctcggcg gcgtaatggg tgcgtcatcc cacgacgcta cgctgcgata aattccgaac
     2881 cgaaaaacaa aggcgttcac gcccgccgct acaagcagca gcagcgaaat ccagtacatc
     2941 acgttggcgc catactccac cggatcggag atgctcaaca aaactccagt ggccagcagc
     3001 acgccgaacc caatccattt ccacggccga aactgctcga tcacttgcga aacgggcact
     3061 ctccgcatcg cggcgccgag cacgcgtaaa ttcccaattg ccaccatccc gccgaataac
     3121 gcgatgctca gcacatggat gatcaggagc accggataat cgtaagtgcc ttcgcgaagc
     3181 gagacgctcc acgaggtgtg gctgagccat tcgcaaaatt tcagcagccc catttgacga
     3241 atctccgaat gtgccgcgct gatcccagcc gcgttacaga tttccgatcc acaacccgcc
     3301 tagcactacg cagagccaaa gtcccagcga aacgcacgct gcgggtttgc gccacaacgg
     3361 cgaagccgca tcgcgcgaag gttgggtcgc tcttcgaatc accgtgaaat gaaatatcag
     3421 cgccagcgcg aggaacagca tcttattgcg aaatgcggca acttgtacgt actcatgagg
     3481 atcggatgag aaaaggtaag gtcccgtggt caactgaatc gcgattccaa tccatatcca
     3541 tggcgccaaa cttttggcaa ttacagagat aggctggcgc ggaaggcacc agcctagcag
     3601 ccgcaggtcc agaatcagga tcgttcccac cagcaaactg aacccgcaaa tgtgaatggc
     3661 ctcgacgacc ggaaaaaccc attcccagcg cgcaatcgga ttcagcggaa gcgcttctgg
     3721 aattccctga agcaggtgaa ggacgaaaac agcggcccac attatccgat gtaaccgatg
     3781 gaccgtccac caagccccac gccggcccac agcagcagcg aaaccaccgc cgccactttg
     3841 caccatatcg ggtgaacgcg tccctcgtcg gagagcgtga tccgccggtg aatggtgaag
     3901 gtgaaaataa tcgccagtag aagcgacgtc attttgaagc ggaagcccca attcccgtac
     3961 atcttcacgg cctcagtgga gaaaagtaga aatccggaaa ttagcattac ggccaggctc
     4021 cccagcgtcc acggccacac atcctgcgct aactcggacg tgggctcgtc ggcaaaccgc
     4081 aagccgagca ggcgcgcgtt caccagcaga attgtgccgc ccaatacgcc gagtgcaagc
     4141 agatgaaata tttcgacaaa gggaaaaagc cacacggaat cgcggatccc gtggccgaac
     4201 cacgtctcat tgcaccagtg gcagaacgac agcagaatca ccttaatcca agtcatttcc
     4261 cgcggacctc ctcacggcgg cggcagagca tatgcgatgc ctcgtccagc agccacgatg
     4321 gccacccaca aaaccagcga aaccaccgct gcaatcttcg ctgccctggg cgtcctcact
     4381 gcgcgatccc attcagctgt cctgcgtccg atcgtcgcgt taaaaatcaa cacgttcagc
     4441 cccgccagca ccagcatgat cattttcacc ttgaagaaag tggtgctgta gaaagatacc
     4501 ggatcgccgc agaaaagcag cacgccgctg ataatcatca ccacgaagcc ggcaatcagc
     4561 caggggttga gttgccgcaa aacttcagaa accggcctgc gccgcatgct cacgcccaga
     4621 aggcgcaaat ccagcaacac ggcgaatcca agaaagagag taagcgttag aacgtggatt
     4681 gtttcaacaa tcggttcggc ccagagcgaa tctcgcatgc tcatgctgaa ttgcgtgtcg
     4741 ctcagccact gcaaaaaact agcgaaccac atccgttacc ctctgggaat ttctttttcc
     4801 tggctttgcc cgagcggcgc gcaagcgaac tactttgcct cgcacggcgt gggattccat
     4861 ccggcgttgt cggcctgctt cttgaattga gagctcgtaa tgtagggctg cgccagatat
     4921 tcggggtcgt ggacgattgt cgtcaccacg agccaggtga taccgcgctc ctcaaagcta
     4981 tcgaaatatt cctcgacgct tgtatttgcg ctgtacggca cgccgttcct ccgtatgtat
     5041 ccgggacgaa catgcgtcgt catcaccttc atgtagcctt cttcttttgg cttggccgcg
     5101 gccggagtgc cctgagccac aggcactact ctgccgcgcg gtcgcatccc ttcccaaccg
     5161 gccacggaat atccctgcca ggttggcggt ccgctcggcg gtttcgtttc gtcgaaatga
     5221 aatatccggg tctgttcgcc ggcatccgtt tccacgcgca aggtgtcgtc attcacccac
     5281 gaaatatgca gccgttccgg cacccgcata attgcggcag ctccatacgc cttgcacgca
     5341 tttccggaag cctgatcctt ggccgggtcc cattcctccg cgatttttcg gccttccgcg
     5401 ttcaccggca ctcccggaaa atcgccttta tcgggagtaa ccatccgata gcgccagtct
     5461 tccgtaacca ccgccaccca gtagccgctc ggatcaaagg gagcatcggt atgcgggtta
     5521 agtttggggg gcggtggacc cgcgtttggc ccaaactggg cgcgggctgg cgcggcgaat
     5581 gccagcacgc caagaatgct aaacaacgcc gcgatacccg accatccatt ccaccgtttc
     5641 attcgatccc ccgccaattg gcccttatca ctcgcggctg ccatgcgacg gctagccgcg
     5701 aaaacaccta catgattacg ccttcccact tccagccgtc tgacggccgc acaattttgt
     5761 gcaagcgaat tctgtgctcc gctggatcgc gccccggatt gccggtcaca accacgtgat
     5821 cgcccggttt cagcgtatcg cggctaacgc ggtcccgagt aagctgggct ccaccacccc
     5881 actcgaccga ccatgtctgc atttgaccct tgctgtcggg cgcctcaact ttcacgaacg
     5941 agtgcggatt gcggtacatg aattgcgtca gcgttccctc gacggtgatc tcttgatcca
     6001 cgaagtaggt tgcagcgaac gaatgatgcg cgtacgcgcg ccctcccatg accaatgcag
     6061 cagctactgc taataggatg aaaactgcgc gtttcataat cggcctccta tacgttcccg
     6121 aa
//
LOCUS       DATNDT010000019         8344 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3695528, whole genome shotgun sequence.
ACCESSION   DATNDT010000019 DATNDT010000000
VERSION     DATNDT010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8344
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3695528"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..1441)
                     /locus_tag="VN788_00725"
     CDS             complement(71..1441)
                     /locus_tag="VN788_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbosone dehydrogenase family protein"
                     /protein_id="HXU19085.1"
                     /translation="MKRFYQFVLIALGLVMLLFVVARNKRAIAAQFTRPPAHAVDPNA
                     KPATPGVFTDFKGEEPGKIHHITAADIPGPYATKSAAIFSRPVPKPADAWPQAPAGFK
                     VTLYAEKLADPPRTIVTAPNGDFFVVSTNKGKVLVFHGMTADGKPEKTEEFASGLKLP
                     FGIAFYPPGPNPKYVYIGDTDAVVRYPYQNGDMKARGAQEVVVSNLPAGPNHFARGVA
                     FSLDGKQMFISVGSHSNVPPESDPDPDPLEFHRADILVADPTAVNQDGKDLQVYASGI
                     RNGSGIAIDPKTGELWTSVNERDEIGDHVPPDYITHVQKGGFYGWPWYYTGGHLDPRF
                     PGKHPELKDKVIVPDVLLEPHNASLNITFYEAKQFPKQYQGQLFAAEHGSWNRSVRTG
                     YEVIFVPMKNGKATGAYEDFLTGFLTPSGDCWGRPVGVAVAPDGSLMVTDDGSNSIWR
                     VTYGKK"
     gene            complement(1506..2726)
                     /locus_tag="VN788_00730"
     CDS             complement(1506..2726)
                     /locus_tag="VN788_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU19086.1"
                     /translation="MIERSERERPPARPAPERPAKHSVLVAFIALVIIAVAVAAVVVP
                     RRHARAALRSETYDLAIPSVSVIHPKLGAPQTEIVLPGNIQAFTDSPIYARTNGYLKN
                     WYADIGARVKKGQLLADIETPEVDQQLDQARADLNTAQANFRLSQITASRYQDLSKTD
                     AVSKQDVDNAKGDFEAKKAMVDSALSNVKRLEQLQSFEKIYAPFDGVITARNTDVGHL
                     INSGAGGPATELFHIAALGKLRVYVNVPQQDSISAKPGLKADLTFQEFPGRKFRGTLV
                     RTADSIDPASRTLLAEVDVDNPTRELLPGSYAEVHLQVPSGSPAFILPVSTLIFRSRG
                     LQVATVDANNHAALKNITLGRDLGNEVEVVSGISAADSIIETPPDSLVTGETVRVVTA
                     ASIPQREPAGNSQE"
     gene            complement(2785..5955)
                     /locus_tag="VN788_00735"
     CDS             complement(2785..5955)
                     /locus_tag="VN788_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXU19087.1"
                     /translation="MWIVRLALRRPYTFVVAAILLLIVGAAAILRTSTDIFPNIDIPV
                     VSILFNYGGLSPEEVSDRIVYNFERSLTTTVNDIEHIESQSLPGRAIVKVFFQPGVQI
                     SIAVAQVTAIAQPSVHSMPPGTNPPFIIQYNASTVPILQLALSGQGLTEQQLGDLGQN
                     FVRVGLITVPGVAIPYPYGGKQRQIQVDLNTQAMQAKGLSALDVVNAVDTQNLILPAG
                     TAKIGSYEYQVDMNGAPQTIRELNDLPVKQVGNSTIYIHDIANVRDGYPPQTNIVRVD
                     GERSALLTILKNGDVSTLDIIANVKKMLPKIKAGLPPQLKIEPLSDQSIFVKASIDGV
                     LREGAIAACLTGLMILIFLGSWRSTLIIAVSIPLSVLTSIIALSALGQTINIMTLGGL
                     ALAVGILVDDATVAIENINRNLDQGKEIVQSILDGSQQIAMPALVSTLSICIVFVPMF
                     FLSGVAKYLFVPLAEAVVFAMLASYLLSRTLVPTMARYMLLERTEEQRRENTQKSRNP
                     FVRMQARFEEGFDRFRESYHRILQGAIRRRKIFAICFLGACVLSFALLPLVGEDFFPS
                     VDSGEFTLHMRAATGTRIEETAALCDRAENDIRSQVPPSELKTIIDNIGLPYSSLNLS
                     YSNSAPIGAGDADILVELAPQHRPTNDYVRALRVKLNHDFPGVEFYALPTDIVTKILN
                     FGLPAPIDIQILGRNILGNRQFADHLLNELKFVPGTVDLRIQQKFDEPKLHIEMNRTK
                     AQEIGFTARDVAQNVLISLSGSFQTQPTFWLDPSTGVSYNITTQTPEYRRDSLQKLQN
                     IPIVAPGTNQSDILSSLASFSRGSEMAVVSHYNVEPAIDIYGAVDGRDLGAVARDIQP
                     IVDRSRKDLPRGSEINVLGQIQTMNSSFIGLLSGLGFSIVLVYLLIVVNFQSWLDPFI
                     ILMALPAALAGIVWFLFITHTTVSVPALTGAIMCMGVGTANSILVVSFARERLEAGKL
                     PGEAALEAGFTRFRPVLMTALAMIMGMLPMALGLGEGGEQNAPLGRAVIGGLLFATVA
                     TLFFVPVVFSIVHGRHEKDQPASTQS"
     gene            complement(6196..6684)
                     /locus_tag="VN788_00740"
     CDS             complement(6196..6684)
                     /locus_tag="VN788_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19088.1"
                     /translation="MRELLSDWNNLLPAAGLAVLAGTLVVFWLRRKPTTPNDLEYQRR
                     AHINRVGRIVEGQVLEIVDRSRAANSGPADDDKEKAGGRQIGLLTKRQGLAEDGSPQK
                     LLYYTYAISGVTYETAQDITGLEERFHLSRITSGQTASVKYDPSNPSNSILLADDWSG
                     LH"
     gene            complement(6869..7684)
                     /locus_tag="VN788_00745"
     CDS             complement(6869..7684)
                     /locus_tag="VN788_00745"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HXU19089.1"
                     /translation="MKLCRFQPLDSAKEQPRWSAREAYLESRAGVLEGDTIREISGEL
                     WGQREPTGREWKLADVRFLPPSQPTKIVCLGRNYHEHAAEMGSVAPKEPLIFLKPPSA
                     IIAPEEPIVLPRISKRVDYEGEIAIVIGRRCSRLADSDDPRPYIAGYTCLNDVTARDL
                     QKLDVQFTRGKGFDTFCPFGPVLESERPPSDAKIETFVNGAGKQSARLDEMVFSMDVV
                     TRFVTQVMTLEPGDVISTGTPAGVGPLAPGDVVEVRVGGIGTLRNTVTGPENS"
     gene            complement(7681..8166)
                     /locus_tag="VN788_00750"
     CDS             complement(7681..8166)
                     /locus_tag="VN788_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19090.1"
                     /translation="MTDAALTENVRSEPDARGPILRTGIYTGALLIIVMWVALVAANR
                     MANLEPYALERNAASYGLFLLFMLIPIARFWNRPFRMFASAAIAWVLFAATYEAAGMV
                     FHNLIDSLRHSPLSVLMEGIFAYGLFAVGSWVMEMAIHARHHPIAPARKPVREAARHI
                     R"
     gene            complement(8163..>8344)
                     /locus_tag="VN788_00755"
     CDS             complement(8163..>8344)
                     /locus_tag="VN788_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19091.1"
                     /translation="VPPKRLNTELLLTPAFRDMNVLSGHVYSYTVTAVDSSGNESGSS
                     AAVSGNVPAERSLTP"
BASE COUNT         1861 a         2203 c         2529 g         1751 t
ORIGIN      
        1 gcttacggct gccgcgaagc ggatgcaccg cgaggtatgt ttttccaacc cgtgaccgaa
       61 aaccacgatc ctactttttc ccgtaagtga cgcgccagat cgaattcgag ccgtcatccg
      121 tcaccataag ggagccatcc ggggcaaccg cgactccaac gggccggccc cagcaatctc
      181 ccgacggggt gagaaagccg gtgagaaaat cctcgtacgc gcccgtggcc ttgccattct
      241 tcatgggcac gaaaatgact tcgtagccgg tgcggacgga gcgattccac gagccgtgtt
      301 cggcggcaaa tagctgcccc tgatattgct tggggaactg ctttgcttcg tagaaggtaa
      361 tgttcagcga agcattgtgg ggctcgagaa gcacatcggg aacgatgact ttgtctttga
      421 gctctggatg cttaccggga aagcgaggat cgagatggcc gccagtgtaa taccaaggcc
      481 aaccatagaa gccgcccttc tggacgtgag taatgtaatc gggagggaca tggtcaccga
      541 tttcgtcgcg ctcatttacg gacgtccaaa gctcgccggt ttttggatcg atggcgatcc
      601 cggagccatt gcggatgcca gaggcgtaaa cttgcaaatc ctttccatcc tgatttacag
      661 ccgtgggatc ggcaacgaga atgtcagctc ggtggaattc cagcggatcg ggatccggat
      721 cggattcagg cggcacattg ctgtgcgagc cgacggaaat gaacatttgc tttccatcga
      781 gggagaaggc cacgccgcgg gcgaagtgat tcgggccggc aggaagattg gaaaccacga
      841 cttcttgagc accgcgggcc ttcatatcgc cattttggta ggggtagcgg acaacggcgt
      901 ccgtgtcacc gatatagacg tacttgggat ttggccccgg gggatagaac gcgatgccga
      961 aaggaagctt caggcccgag gcgaactctt cagttttctc cggcttgcca tcggcggtca
     1021 tcccgtgaaa gacgaggacc ttgcccttgt ttgtggagac gacgaagaaa tcgccattgg
     1081 gagcggtgac gatggttcgc ggcggatcgg ctaatttttc ggcgtagagc gttaccttga
     1141 agccggccgg cgcttgcggc caggcgtccg cgggtttggg aacggggcgc gagaaaatgg
     1201 ccgcggattt cgtggcgtag gggcccggta tatcggcagc cgtaatgtga tggattttgc
     1261 ccggctcttc gcccttgaaa tcggtaaaca cgcccggagt ggcgggtttg gcgttcgggt
     1321 ctactgcgtg tgccggcggc cgggtgaact gcgcggcgat cgcgcgttta tttcgcgcga
     1381 cgacaaataa gagcattacc aggcctaaag cgatcagcac gaattgataa aaacgcttca
     1441 ttgctcattc cccctttaat ttcgatactt cctaaacttg aattttacgc gaagctgctc
     1501 cccattcatt cttgagaatt tccagccggc tcgcgttgcg gaatacttgc ggcggtgact
     1561 acgcggacgg tttcaccggt aaccagggag tccggcggag tttcgataat ggaatcggct
     1621 gctgagatgc cggatacgac ttcgacctcg tttccgaggt cacgaccgag ggtgatgttt
     1681 ttgagtgcgg cgtggttgtt ggcgtccacg gtagccactt gcaggccgcg agagcgaaaa
     1741 atcaaagtgc tgacgggcag aatgaaagcg ggactgccag agggcacttg gaggtggact
     1801 tccgcgtagg agcccggcag aagctcacgc gtcggattat caacgtccac ctcggccagg
     1861 agagttcgcg acgcaggatc gatggaatcg gccgttcgaa ccaaagtgcc cctgaacttg
     1921 cgtcctggaa attcctgaaa agtgagatcg gccttgaggc cgggcttggc ggagatggaa
     1981 tcctgttgcg gcacattgac ataaacgcgc aattttccca gcgcggcgat gtggaacagc
     2041 tccgtggcag gaccgccggc tccggaattg atgaggtgac cgacgtccgt atttctggcg
     2101 gtaatcacgc catcgaaggg tgcatatatt ttttcgaagg attgcaattg ctcgagccgc
     2161 ttcacattgg agagggccga gtccaccatg gcctttttgg cttcgaaatc gcccttggcg
     2221 ttgtcaacat cctgcttgga taccgcatcg gtcttcgaaa gatcctggta tcgcgaggcg
     2281 gtgatttgcg acaggcgaaa attggcttgg gcggtattca agtcggcgcg agcttggtcg
     2341 agctgttgat ccacttcagg agtttcgata tccgccagca attggccttt tttcacgcgg
     2401 gcgccgatat ccgcatacca atttttgaga tagccgttgg tacgggcata gatgggtgaa
     2461 tcggtgaaag cctggatatt tccgggcaga acgatttctg tttggggcgc gccgagcttg
     2521 ggatgaatta cggaaacgga tggaattgcg agatcgtagg tctcagacct tagggccgcg
     2581 cgggcatgcc gtcggggaac aacgacggcg gctacggcga cggcaatgat caccagggcg
     2641 ataaatgcga ccagcacact gtgcttggct gggcgctccg gggcaggccg cgcgggcggc
     2701 cgttcgcgtt cactccgctc aatcatccgc tctttcgtct ccagtattta tcacaatata
     2761 gacggtttgc gcccagcgtc cgtttcacga ttgggtggat gcaggctgat ctttttcgtg
     2821 ccggccgtgc acaatgctga aaaccacggg gacaaaaaag agagtcgcga cggtcgcaaa
     2881 caggaggcct ccgatgaccg cccggccgag gggagcattt tgctcgccac cttcgcccag
     2941 tccaagcgcc atgggaagca tgcccataat catggccagg gcggtcatga gaacagggcg
     3001 gaagcgggtg aaaccggctt caagagccgc ttcgcccggc aacttccccg cctcgaggcg
     3061 ttctctggca aagctgacaa caagaatgct gttggcggtg ccgacaccca tgcacattat
     3121 cgcaccggta agagcgggaa cgctgacggt ggtgtgtgta ataaacaaaa accagacgat
     3181 cccggccagc gcagcgggaa gggccatcaa aatgatgaat ggatcgagcc aggattgaaa
     3241 attcacgacg atcaacaagt acacgagaac gatggaaaat ccgagtccgg agagcaggcc
     3301 gataaaggac gagttcatgg tttgaatctg gccgaggaca ttgatctccg aaccgcgcgg
     3361 cagatctttc ctgctgcgat ctacgatggg ttggatgtct cgggcgacgg cgccgagatc
     3421 gcgtccgtcc acggcgccgt agatatcaat ggcaggctcg acgttgtagt gcgaaacaac
     3481 cgccatttca gaaccgcggc tgaaggaagc gaggctgctg agaatatccg attggttcgt
     3541 gcccggagca acgatcggaa tattctggag tttctggagt gagtccctac ggtactcggg
     3601 tgtctgggtc gtaatgttgt agctgacgcc ggtgctggga tcgagccaaa atgtcggttg
     3661 agtttgaaaa ctgccgctga gcgaaatcag aacgttctga gcgacatcac gcgcggtaaa
     3721 tccgatttcc tgagccttcg tccgattcat ttcgatgtgc aacttgggct cgtcaaactt
     3781 ttgctggatg cgaagatcca ccgtgccggg aacaaatttc aattcgttta ggaggtgatc
     3841 ggcgaactgc cggttgccga gaatattccg gcccaagatt tgaatgtcga tgggagcggg
     3901 gaggccgaaa ttgaggattt ttgtgacgat atcggtcggc aaggcataaa actccactcc
     3961 ggggaagtcg tggttgagct tcacgcgcag cgcgcggacg taatcgttcg tcggccgatg
     4021 ctggggggcc aattcgacca agatgtcagc gtcgcccgcg ccaatgggcg ccgagttgct
     4081 ataagaaaga ttgaggctgc tgtagggaag cccgatattg tcgatgattg tcttcaactc
     4141 cgagggagga acctgcgaac gaatgtcatt ctcggcccga tcgcaaagcg ccgccgtctc
     4201 ttcaatccga gtgccggtgg cagcgcgcat gtgaagcgtg aattctccgc tatcgacgct
     4261 ggggaaaaaa tcctctccga ccaggggcag cagcgcgaag gacagcacgc atgcacccaa
     4321 gaaacaaatg gcgaaaatct tgcggcgccg gatcgctccc tggaggatcc ggtgatagct
     4381 ttcccggaag cggtcaaatc cttcttcgaa ccttgcttgc atgcgcacga acggattgcg
     4441 ggatttctgg gtattttctc ggcgctgttc ctcggtccgc tcgaggagca tatagcgcgc
     4501 catggtggga accagcgtcc gcgagagcag atacgacgcg agcatggcaa agacgaccgc
     4561 ttccgcaaga gggacgaaga gatacttcgc gacgccgctt agaaaaaaca tcggaacgaa
     4621 gacgatgcaa atagagagag tggaaacgag cgccggcatc gcgatctgct gggagccatc
     4681 gagaatggac tgcacgattt ctttgccttg atcgagattc cgattgatat tttcgatggc
     4741 gacggtcgcg tcatccacca ggatgcccac ggccagggct agacctccta acgtcatgat
     4801 attgatggtt tgaccgaggg cgctgagcgc aataatggaa gtgagcactg aaagcggaat
     4861 cgagacagcg atgatcaagg tgctgcgcca gcttcccagg aaaataagga tcatcaatcc
     4921 agtgagacag gcggcaatcg cgccttcgcg caggactcca tcgatcgatg cttttacgaa
     4981 gatcgactgg tccgagagcg gctcgatttt caattgaggc ggcagcccgg ctttgatctt
     5041 ggggagcatc ttcttcacgt tggctataat atcgagcgtc gacacatcgc cgttttttaa
     5101 aatagttagc agggccgacc gttctccatc gacgcgcacg atattcgtct gcgggggata
     5161 gccgtcccgc acattcgcta tatcgtgaat gtagattgtc gagtttccga cctgcttcac
     5221 cggaaggtcg ttgagctccc gaatggtttg gggagcgcca ttcatatcca cttgatattc
     5281 gtaggaaccg atctttgcgg tgcccgccgg cagaatcaga ttctgggtat cgacggcgtt
     5341 caccacatcg agcgccgaaa gccctttggc ctgcatcgcc tgggtattca aatccacttg
     5401 aatttggcgc tgtttgccgc cgtaggggta tggaatcgca acacctggga cggtgataag
     5461 acccacgcgc acgaagttct gcccgaggtc gcccaattgc tgctcggtga gtccctggcc
     5521 cgaaagagcc agttgcagaa tgggaaccgt agaagcgttg tattgaatga tgaacggggg
     5581 gttcgtcccc gggggcatgg agtggacgga aggctgagcg atggccgtaa cctgtgcgac
     5641 cgcaatcgat atttgaacgc ccggttgaaa gaacaccttc acgatcgcgc gtccggggag
     5701 cgattgagat tcaatgtgct cgatatcgtt caccgtggtg gtcagagaac gctcgaagtt
     5761 atagacgatg cgatccgaca cttcctcggg tgagagccct ccgtagttaa agagaatgct
     5821 gacgacaggt atatcgatat tcgggaaaat atcggtggaa gttcgcagga ttgcggcagc
     5881 gccgacgatc aacagcaaaa tggccgccac cacgaatgta taagggcggc gtaatgcgag
     5941 tctgacgatc cacatttaaa aaggctcctg aaatggccct cgggaaagag cacgcgtcgt
     6001 attcccgccg aattgaccga atgcgccggc gccattatct cacgcggcat tcggaatcga
     6061 aatcaggagg agcgggtcga aaattccggg ctaggccggg gccagcacga ttcagacgtg
     6121 caacgagttc gcttcggtaa aaattttaaa attggtgtta cctggggaag agcctaaatt
     6181 cggcgctaag gaattttaat gcaaacccga ccagtcgtcg gcgaggagaa tggaattgct
     6241 cggattcgac ggatcgtatt tcacactagc agtctggccg gatgtgatgc gcgataaatg
     6301 gaatcgctcc tcaagccccg tgatatcctg cgcggtctcg tacgtgacgc cggaaatggc
     6361 ataggtgtaa taaagcagct tttgagggct gccatcctcc gcgaggccct gccgtttagt
     6421 cagaaggccg atctgccggc cgccagcttt ttccttgtcg tcatccgccg gtccggaatt
     6481 cgccgcgcgg ctacggtcaa caatctccag cacctggcct tcgacaatcc gcccaacccg
     6541 gttgatgtgg gcgcgacgct gatattcgag atcgttggga gtcgtcggct tgcggcgaag
     6601 ccaaaatacg acgagcgttc ctgcgaggac ggcaagaccg gccgccggaa gcaaattatt
     6661 ccagtcgctt agaagttctc tcaaggagag ggattactct caaggccgcc agtaaagctc
     6721 caaattgaat tggcggcaaa gtcgtttcac ttccgactat aaatctatac gcaaatgatt
     6781 tccgtctcgg aaagcaaact gcggttggtt tcctgacagt tacggcatgt cgcgtgcgat
     6841 gtcttctgga gggcaagccg ggctcgacct aggaattttc cgggccggtt acggtgttgc
     6901 gaagcgttcc aataccacct acacgaactt ccaccacatc gccgggcgcc agggggccca
     6961 cgccggcagg ggtgccggtg gaaataacgt cgcccggctc gagcgtcatt acctgggtga
     7021 caaaccgagt aacgacgtcc atcgagaaga ccatttcatc caggcgcgcc gattgcttgc
     7081 cggccccgtt gacgaaagtt tcaattttcg catcggaggg aggccgttcg ctttccagga
     7141 cgggaccaaa cgggcaaaag gtatcgaagc ctttgccccg tgtgaattgc acgtccagtt
     7201 tttgcagatc ccgcgcggtc acgtcgttca agcaggtgta gccggcgata taggggcgcg
     7261 gatcgtcgct atcagcgagg cgcgagcagc gccggccgat cacgatcgca atctcgccct
     7321 catagtccac tcgctttgag atgcgcggaa gcacgatggg ctcttctggg gcgatgattg
     7381 cggacggcgg cttcaaaaag atcaatggtt ccttcggcgc cacgctgccc atctcagcgg
     7441 cgtgctcgtg ataatttcga cccaggcaaa cgatcttagt gggctgagag ggcggcagaa
     7501 atcgcacgtc cgcgagcttc cactcgcgtc ctgttggctc gcgctggccc catagttcac
     7561 cggaaatctc acgaattgtg tcgccctcca ggacgccggc gcgcgattcg agataagcct
     7621 cgcgggcgga ccagcgtggc tgctctttcg cggaatcgag cggttggaag cggcagagct
     7681 tcatcgaatg tggcgcgctg cctcgcgaac gggcttgcgg gccggtgcaa tcggatgatg
     7741 gcgcgcatga atcgccattt ccatcaccca tgatccgacg gcgaagagcc cgtaagcaaa
     7801 tattccttcc atgagcactg agaggggact gtgccgcagc gagtcaatga ggttgtgaaa
     7861 aaccattccg gccgcctcat acgtggcggc aaacagaacc catgcaatgg cggccgatgc
     7921 aaacatgcgg aacggccggt tccagaagcg tgcaatcgga atcagcataa aaagcaggaa
     7981 caagccatac gaggccgcgt tccgctcgag cgcgtacggt tccaagttcg ccatccgatt
     8041 cgctgcgacc agggcgaccc acattacgat gatcagcaaa gcgccggtgt aaatgccggt
     8101 ccggagtatc ggtccgcgag cgtccggctc agatcttacg ttttcggtta acgctgcgtc
     8161 tgtcatggtg tcaagcttct ttcggcgggc acgttgccgg agacagccgc gctggaaccg
     8221 ctctcgtttc cagaactatc aacggcagtt acagtatagg agtaaacgtg gccggacagg
     8281 acattcatat cgcgaaaagc cggcgtaagc aacagttccg tgttcaaccg ctttggcggg
     8341 acac
//
LOCUS       DATNDT010000020        10443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3692272, whole genome shotgun sequence.
ACCESSION   DATNDT010000020 DATNDT010000000
VERSION     DATNDT010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10443
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3692272"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(324..911)
                     /locus_tag="VN788_00760"
     CDS             complement(324..911)
                     /locus_tag="VN788_00760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19092.1"
                     /translation="MMNRILYGLIVFSLFSPVVAAQGYSANHAEFGVYADYFRLHQTD
                     TNLGGVGARLSVNTTPYLQFEAQLAYDFEQGFTERFRPFGGTLFVQRSDLRLLHGSFG
                     PKLQTPGPVRLFVTAKGGFDNFGFSNAPATLGTFTSTVSDLRADNLNAVFYPGGGVET
                     FLGPIGLRLDVGDEMYFNHGAHHNLSITFGPTIRF"
     gene            complement(1010..1645)
                     /locus_tag="VN788_00765"
     CDS             complement(1010..1645)
                     /locus_tag="VN788_00765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU19093.1"
                     /translation="MKANEKGPGENALPAPARDQAPHPDTSELVALAQAGDELAFSRL
                     VQPHMRKIYHVALRITRNREDAEDASQQTLLKAFANVGQFHGASQFSTWLIRIAMNEA
                     LMTIRKRHSEDSHLCYDANPTGESTYLQDLCAGDSAQPEVLYVKSENRRLLREAIGGL
                     RGTLRVVVCLLGLEEHQSKDAARILNLSQSAVKTRFLRARQELRECLGERI"
     gene            2044..3027
                     /gene="galE"
                     /locus_tag="VN788_00770"
     CDS             2044..3027
                     /gene="galE"
                     /locus_tag="VN788_00770"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HXU19094.1"
                     /translation="MAILVTGGAGYIGSVMVERFREKGKAVVVIDDLGRGHRSAVDPD
                     VPFYRGKVGDRAVVTRATQEHKIEACVHFAALAYVGESVSEPARYFENNVAQGIALLD
                     VLVSAGVKRIVFSSTCATYGEPKQIPIAETCPQRPVNPYGWSKLMVERMLESYDKSYG
                     LRFVALRYFNAAGATENHGEHHEPESHLVPNVLSAALGEAQALSVFGNKYPTPDGTAI
                     RDYVHVSDLAEGHICALDYLERTGKSDFFNLGTGRGYSVLEVIECARKVTGSPIPVQI
                     APPRPGDPPELVADAAKAGDVLRWKPAMSDLPSILRSQWEWRRKHPRGYAD"
     gene            3131..4993
                     /gene="treZ"
                     /locus_tag="VN788_00775"
     CDS             3131..4993
                     /gene="treZ"
                     /locus_tag="VN788_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HXU19095.1"
                     /translation="MDLPTRIDERTIGAIPLADGSWHFSVWAPKQGRVSLQVFGGNEQ
                     VVRMEKDERGYHCAALSNLAAGDRYLYQLDDTHERPDPASRFQPDGVHGPSQLVDLGA
                     FEWSDSNWKGRPLGTSIFYELHVGTYTRAGTFDGIIQRLPELSDLGVTTIELMPVAQF
                     PGSRNWGYDGVYLFAPQNSYGGPYSLQRLVDAAHAHGLAMTLDVVYNHLGPEGNYLGE
                     YGPYFTEKYRTPWGKAINFDDAQSDPVRQYFIQNALYWLKEFHFDALRLDAIHGIFDF
                     SARHFLAELQDEASALAKKLGREIHLIAESDLNDARLLLPHEKGGYDLPAQWSDDFHH
                     SLHTLLTKEHTGYYGDFGRVHHLASVIKDGWYYHGQFSKYRQRRHGNSASGITKSHFV
                     VCSQNHDQVGNRALGERLSQLVNFEGQKLAAGVTLLSPFVPLLFMGEEYGETSPFLYF
                     TSHGDKDLIEAVRRGRREEFASFGWEHETPDPQALATFEASALKHELEEQESHRTLRT
                     FYRELIELRNTCHLGVDSNVEPTEDEAHECLRLLWTSERNAMAALFFFGSTELQLEAK
                     LPPGRWLTKLNSADSKWLGPGSELTEGAVVNPSSRISVSPQSFLVLERAGTGRS"
     gene            5066..7948
                     /gene="treY"
                     /locus_tag="VN788_00780"
     CDS             5066..7948
                     /gene="treY"
                     /locus_tag="VN788_00780"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR02401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HXU19096.1"
                     /translation="MSLKRIPVSTYRLQFNEGFRFQDALRLLDYFSTLGISDLYASPL
                     LVSRHGSGHGYDVTDPAHIDPEIGSEEDFEALQNGLLNRGMRLLLDIVPNHMAASSEN
                     RWWMDVLENGAESAFASYFDIQWHPASRNLDGKILLPVLARPFGETLDGGELRLIFQN
                     GKFLIQYKESLFPLAPRTYNHVLKEHLDDLKKILGEGSPACEEYLGILSTLSSLSEKD
                     RVHSTAGERRLRFEAGRDRLKLLTENHSEVRAFMEANLSRFNGDLRDPASFCHLERLL
                     AEQNYWLAYWQDANEGINYRRFFAISDLVGMRVEDPIVFEAMHEQIFRLIGKGAISGV
                     RIDHIDGLRDPAGYLNRLSERIASTVSTDGGKPYVIVEKILAPHETLPEEWPVAGTTG
                     YDYLNWANGIFVSPAGAKRLAGIYSGFTGKEEKFADILYEKKKLVMNSLLRVEMRSLG
                     RQLAELAGKDRYARNILRSELTDALVETTACMDVYRTYVRNLDIPPAARERIETALEQ
                     ARIRRPRLSAECFAFLREILTLRNPAHVLPDQREERLAFVMRWQQLTGPIVAKGLEDT
                     ALYVYYPLLSLNEVGGRPSLSSIVSREEFYALMQERLRRWPHSLNATSTHDTKRSEDI
                     RARLNVLSEMPEEWGQQIAKWAACNEKHKQLIEGNKVPDANEEYLIYQTLIGLWPLDF
                     SNVSSITQRLQAYLVKATREAMINTRWTEPNKTHESALAQFIEELLSPKNEGFVRDFN
                     AFQKKIAYCGMINGLAEVLLKITCPGVPDFYQGSELWDFRLVDPDNRGRVDFGERSRL
                     LEAVVRESDKDLACGARDFVEHWPDGRIKLHVIRQTLAYRRKYDELFSKGDFIPLEAR
                     GNRAEHVVAYLRSLGELCALTVVPRWLAATQAEPPQMDLREFWQDTALLLPRETSRSW
                     RNIFTHREIGSQLFGDNQTLLLGDLLAQFPVAVLTSADSESPGVRN"
     gene            8135..9808
                     /locus_tag="VN788_00785"
     CDS             8135..9808
                     /locus_tag="VN788_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HXU19097.1"
                     /translation="MAMITGGELVVRTLIQANVKNVFGLHGAHLETIFQSCLDHRIPI
                     TDTRHEVAAGHAAEGYARSTRSLGVAMATAGPGFTNIITSIANAYLDRTPVLYITGSS
                     GLAEAETNTLQSGLDQVAVVTPITKWAHRISVTQQIPRLVAQAIRLATSEPMGPVLLD
                     MPMDVLGAQVEEASVQIPEKVLVDARSEPQSSAIDEALKLLASAARPVIMVGVGAYQS
                     GAEEELRAFAEATGTPVFSDFQAHGMLPSNHPLYGGTFHKMADLSAPDQRPDLVLALG
                     VRFGLFTLGMSDLVVPLAAKIVHVEIDPKEIGRLRQVAVGIVADSRETLHAMNARAKS
                     QKWPDRSRWQQSIRNAKVERCKRAQADLNRTTTPIHPLQAVSAIVDNIPENTIVVGDG
                     AEAYHWMNEVIRQNSPGSYITHGFLGAVGMGLGLSIGVQVANPDRPVLCLVGDGAIGF
                     TIAEFDTMMRHQLPIVVAVMNNHSWAASQHFQEIVSGKDRLTGTELRDAKYHEVAAAF
                     GCHAQRVTELQELGPAIKAAFASGKPACVNVAIDVVPLPPELHLLMARH"
     gene            9823..>10443
                     /locus_tag="VN788_00790"
     CDS             9823..>10443
                     /locus_tag="VN788_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXU19098.1"
                     /translation="MQVRRLGKNGPEVSAIGLGCMGMSGTYGAADETESIATIHAAIE
                     AGVTLFDTGDFYGMGHNEMLLQKALAGRPRDRFLIGVKFDGMRGPNGAFIGLDARPVA
                     VKNFLAYSLRRLGTDYVDLYQPARVDPSVPIEDTVGAISEMVKAGYVRHIGLSEASAD
                     TIRRANAIHPIAALQIEYSLVSRSVEAEILPALRELGISLVAYGVFS"
BASE COUNT         2320 a         2802 c         3088 g         2233 t
ORIGIN      
        1 gcttcgccac atacctccca gcccgggtgc gattggagga gggcctttac tccacggcgc
       61 atcagatcgt ggtcatctgc gattaagata cggagatttc tcacggttca ctcctgaagg
      121 cgatgctggg accaactcgc gaacaagcgc cggagaggta gccggcacat tctgccgcgc
      181 tggaagcacg attatacaca acaaggggag tcaaggcgcc gttctttctg cgcggaagta
      241 ggatggccga caagccacga acgtcggtcc ctgcataact ttaaagtggc aatgattggc
      301 agagggtctc actatccgta aatctaaaag cgtattgtgg ggccgaaagt gatgcttagg
      361 ttatggtggg cgccgtggtt aaaatacatt tcgtcgccga cgtccaagcg caagccaata
      421 gggcctaaga acgtctcgac gccaccgcct ggataaaaaa cagcgttcag attgtccgca
      481 cgcaagtccg atacggtgct ggtaaaggtt cccagcgtcg ccggcgcatt gctgaagcca
      541 aagttgtcga agccaccctt cgcagtgaca aaaagtcgaa ccgggccggg agtctgcagt
      601 ttaggcccaa aagatccgtg cagcagacgc aggtccgatc gctggacgaa gagcgtccca
      661 ccgaatggcc tgaatctctc cgtgaacccc tgttcgaagt catatgccag ctgcgcttcg
      721 aactggagat acggagtggt gttcacggag agccttgctc caactccgcc caaatttgta
      781 tcggtttgat gcaagcggaa atagtctgca tatactccga actccgcgtg gttggctgaa
      841 taaccctgcg ccgctactac cggcgaaaag aggctgaata caatcaaacc atacaagatt
      901 ctgttcatca tggtatcccc cgtttgtttg cagttgctcc gcttcattca ggcggcatca
      961 ccgctgcgga cgggagactg aatcgtccta cgaaaacggt ctatgggcct taaatgcgct
     1021 ctccgagaca ttcccgtaat tcctgacgag ctcgcaggaa ccgggtcttc acggcggact
     1081 gggacaaatt caaaatcctg gcggcatcct tgctctgatg ctcctcgagg cccaacaggc
     1141 aaaccaccac tcgcagcgtt ccgcgaagtc cgccgatggc ctcccggagc agcctgcgat
     1201 tttcgctttt gacatacaaa acttcaggct gcgcggaatc gccggcgcag agatcctgaa
     1261 gataagtgga ctcgccggtt ggattggcat cgtagcaaag gtgtgaatct tccgagtggc
     1321 gcttgcggat ggtcatcaaa gcttcgttca tagcgatgcg aatcagccac gtggaaaact
     1381 gcgaagcgcc atgaaattgg ccaacatttg cgaaggcctt caaaagagtc tgctgagagg
     1441 catcttcggc atcttcacgg ttgcgggtga tccgaagagc gacatgataa attttgcgca
     1501 tgtgaggctg gacgagacgc gaaaatgcca actcgtcgcc agcttgtgcg agtgcaacca
     1561 gctcggaggt atccgggtgt ggcgcctggt ctcgcgcagg ggccgggagt gcattttcgc
     1621 ctggaccttt ttcattagct ttcatcacgc ctcacctcga ctcgtagctt ttagttgcgc
     1681 ggccgacttt tcgactctat gaacttaggc caaagatcct ggtgccgttc gggcgggccg
     1741 ccagcaaagg aaaggacagc tataaagcaa tcgcgggcga gtaatcacgc ctatacaacg
     1801 tcactcaact gggaggatag tgtgaacaac cgaatgctgc catcagagtc atgctgtagg
     1861 tctgccagga gtttgctgta tcgctgtcgg gggagattgc ggtcttccaa aacgaacgtc
     1921 atcagctcgt aagtgattac ataaaatatg gttgcctgtc cttgagcagt tgcggcgaaa
     1981 ttggcagaaa agaactctcg attataatta ccattcacta tctcgatggc ggatagaggt
     2041 gacatggcaa tactagtgac gggtggggcg gggtatatcg gcagcgtgat ggtggagcgg
     2101 ttccgcgaga agggcaaagc tgtcgtggtg atcgacgatc tcggtcgcgg ccaccgaagc
     2161 gcggtggacc cagatgtgcc gttctatcgc ggcaaagtgg gagaccgggc ggttgtcacc
     2221 cgagctacgc aagaacataa gattgaggcg tgcgttcact ttgcggcgct tgcctacgta
     2281 ggtgagtccg tttccgagcc agcgagatat ttcgagaaca acgtggctca aggaattgca
     2341 ttgttggacg tgttggtgag cgcgggcgtg aagcggatag tgttttcctc tacgtgtgcc
     2401 acttatgggg aacccaaaca gattccgatc gcggaaacgt gcccgcagcg gccggtgaat
     2461 ccttacggct ggtcgaaatt gatggtcgag cgcatgctgg aaagctacga taaatcttac
     2521 ggccttaggt tcgtggcgct acgctacttc aacgccgccg gcgcaacgga gaatcacggg
     2581 gaacaccacg aaccggaatc gcatctggtg ccaaacgtat tatcggcggc gctcggggaa
     2641 gctcaagcgc tctcggtatt cggaaacaaa tatccaacgc ccgatggaac cgcgatccgc
     2701 gattacgtgc atgtgagtga cctggcagag ggccacattt gcgcccttga ttacctggaa
     2761 cgaacgggca aatccgattt cttcaacctg ggcacgggcc gcggctactc cgtgctggaa
     2821 gtaatcgagt gcgcgcgaaa agttacgggc agccctattc ccgtgcaaat cgcgccgccg
     2881 cggcccggcg atcctcccga gctggtggca gacgcggcga aagccgggga tgtgctgcgg
     2941 tggaagcccg cgatgtccga tctgccgtcg attctacgct cgcaatggga gtggcggagg
     3001 aagcacccgc gaggttatgc agactgagag aaacctcgag ccagcggtaa cggcgcgatt
     3061 gattgtctcc cttccccgaa gctgaagtat tctggatgag cagatcgcgg aaccacgcac
     3121 cagggccgcc atggacctcc ctacccgaat tgatgagcgc acgatcggag cgattccact
     3181 tgcagatggc tcgtggcact tttccgtttg ggcgcccaag cagggacgcg tatcgcttca
     3241 ggtcttcggc ggcaacgagc aagtggtgcg gatggagaag gacgagcgcg gctatcactg
     3301 cgctgccctg agcaatctgg cggcagggga tcgctatctc tatcagttgg atgataccca
     3361 cgaacgcccg gaccctgcgt cgcggtttca accggacgga gttcacggac catcgcaact
     3421 agtggatctg ggtgcgtttg agtggtccga ttcgaattgg aaagggcggc cgctcgggac
     3481 gagcatcttt tatgaactcc atgtagggac gtacacgcgc gcgggaactt tcgatgggat
     3541 catccagcgt ttgcccgagc tttccgactt gggagtgacg acgatcgagc tgatgcccgt
     3601 ggcccaattt cccggttctc gaaattgggg ctatgacggc gtgtatctct ttgccccaca
     3661 gaatagttat ggggggccgt attcgctgca acgactggtg gacgctgcgc acgctcatgg
     3721 tctcgcgatg acactggatg ttgtttacaa ccatctcggg ccggagggca attacctggg
     3781 ggagtacggc ccgtacttca cggaaaagta cagaacgcct tggggcaaag cgataaattt
     3841 tgacgatgcg cagagcgatc ccgtacggca gtacttcatc cagaatgcgc tctattggct
     3901 gaaagaattt catttcgacg cattgcggct cgatgcgatc cacggaattt tcgatttcag
     3961 cgcgcggcat tttctggcgg aactacagga tgaagcaagc gcgctggcca agaagctggg
     4021 gcgcgaaatc catctgatcg cggaaagcga tttgaatgat gcaagactgc tgctgccgca
     4081 cgaaaagggc gggtacgatt tgccggcgca gtggagcgac gatttccatc actcattgca
     4141 cacccttctg accaaggagc acaccgggta ctacggggat tttgggcgcg ttcaccactt
     4201 agcgagtgtg atcaaagatg gctggtatta tcacggccag ttttcgaaat accgtcagcg
     4261 ccggcacggg aattccgcga gcggcattac gaaatcccat tttgtagtgt gcagccaaaa
     4321 tcacgaccag gtgggaaacc gcgcgcttgg cgaacggctg agccaactcg tcaactttga
     4381 aggacagaag ttagcggcgg gcgtgacgct gctatcaccg ttcgttccgc tgcttttcat
     4441 gggcgaggaa tacggagaaa cttcgccttt tttgtatttc acgagccacg gagacaagga
     4501 tcttatcgag gcagtgcgtc gcggacgtcg cgaagagttt gccagctttg gctgggaaca
     4561 cgagacaccc gatccgcagg ccctggcaac tttcgaggca tccgcgctga aacacgagct
     4621 agaagagcag gaatcgcacc gcacgctgcg aactttctat cgggaactga ttgagcttcg
     4681 caacacgtgt cacctcggcg tggattcaaa cgtcgaacct accgaggatg aggctcacga
     4741 atgcctgagg cttttgtgga cgtccgaacg aaatgcaatg gcggcgcttt tcttttttgg
     4801 cagcaccgaa ctgcaactgg aagcgaagct gccgccaggg cgatggctga caaaactaaa
     4861 ctcggccgat tcgaaatggc tcggtcccgg gagcgagctg acggaaggcg cagtcgtgaa
     4921 tccatcgtct cggattagcg tatcgcctca atccttcctc gtattggagc gcgccggaac
     4981 gggaagaagt tgaagtaatg agagagttcg cattttcgac caatctggaa aacaagttcc
     5041 gcatttggac gggagcgtag gcccagtgtc cttgaagcgc attcccgtat ccacatatcg
     5101 cctgcaattc aatgaaggct tccgcttcca ggatgccttg cggctgttgg attatttctc
     5161 gacactgggc atttcggatt tgtacgcttc gccgcttttg gtgagccgcc atggcagcgg
     5221 gcacggctac gacgtgaccg atccggcgca tatcgatccc gagatcgggt ccgaagaaga
     5281 ttttgaagcg ctgcaaaacg gactgctgaa tcgggggatg cgattgttgc tggatattgt
     5341 gccgaaccat atggccgcca gcagtgaaaa tcgctggtgg atggacgtgc tggaaaacgg
     5401 agcggaatcg gcgttcgctt cttacttcga catccagtgg caccccgcct cgcgcaacct
     5461 ggacggcaag atcctgctgc ctgttctggc acgtcctttt ggggaaacgc tcgatggagg
     5521 cgagcttcgc ctaatatttc agaacgggaa atttctaata cagtacaagg aatcgctgtt
     5581 tcccctggcg ccgcgaacgt acaaccacgt tttaaaggaa cacctggatg atttgaaaaa
     5641 aattctgggc gaaggttccc ctgcatgcga ggaatatctg ggaattttat ccacgctatc
     5701 gtccctttct gaaaaagatc gcgtccactc gacggcgggt gaaaggcggt tgcgtttcga
     5761 ggcgggccgg gaccgactca agctcctgac ggaaaaccat tcggaagtgc gggcattcat
     5821 ggaggcgaac ctctcgcgat ttaacggcga cctgcgcgac cccgcaagct tctgccacct
     5881 tgagcggctt ttggccgagc agaactactg gctcgcgtac tggcaggatg cgaacgaagg
     5941 catcaactac cgcaggttct ttgccatttc ggatttggtt ggcatgcgcg tggaagatcc
     6001 gatcgttttc gaggcgatgc acgagcagat tttccgcttg atcggcaagg gggccattag
     6061 cggagttcga atcgaccata ttgacggatt gcgcgatccg gcaggttacc ttaatagact
     6121 aagcgagcgc atcgcgtcca cggtatctac ggatggcggg aagccttacg tgatcgtaga
     6181 aaaaatactt gcgccgcatg agacgttgcc cgaggaatgg cctgtcgctg gaaccacggg
     6241 gtacgactat cttaactggg cgaatggcat ttttgtgtct ccggccggcg ccaaacggct
     6301 ggccggaatt tattctggat ttaccggcaa agaagagaag ttcgcggaca ttctttacga
     6361 gaaaaagaag ctggttatga acagccttct gcgcgtggag atgcgatcgc tgggcagaca
     6421 actggcggaa ttggcgggca aggatcggta cgcgcggaat attttgcgct cggaattaac
     6481 tgatgcgcta gtcgaaacga ccgcgtgcat ggatgtgtat cgaacgtacg tgcgaaattt
     6541 ggatattccg ccggccgctc gggagaggat cgaaacggct ctggaacaag cgcgaatccg
     6601 ccggccgcga ctgagcgcgg aatgcttcgc gttcctgcgt gaaatcctga ctctccgtaa
     6661 tcccgcgcat gtgcttccgg accagcggga ggagcgcctg gcgttcgtga tgcgctggca
     6721 gcagcttacg gggccgattg tggccaaggg attggaagat acggcgctgt acgtttacta
     6781 cccgctactt tcgctgaacg aggtcggggg gaggccatcg ctatcctcca tcgtctcgcg
     6841 ggaagaattc tacgcgctga tgcaggagcg actgcggcgc tggcctcata gcctgaatgc
     6901 cacgtcaacg cacgatacta agcggagcga agacattcgc gcgcggctca atgtgctctc
     6961 ggaaatgccg gaggagtggg gacagcagat cgcgaaatgg gctgcttgca atgaaaagca
     7021 caagcagcta atcgagggca ataaggtccc cgatgcaaat gaagagtatt tgatctatca
     7081 aacactcata ggattgtggc cgctcgattt ctcgaatgtt tcatcaatca cccagcgcct
     7141 gcaggcatac ctcgtgaagg ccacgcgaga ggccatgatc aacaccaggt ggacggagcc
     7201 gaataaaact cacgaaagcg cgctggctca gtttatcgag gagcttctta gtcctaaaaa
     7261 cgaaggcttc gttcgtgact tcaacgcttt tcaaaagaag atcgcatatt gcggcatgat
     7321 taacggcctg gccgaggttc tacttaaaat aacatgccca ggcgttccgg atttttatca
     7381 aggctcggag ctgtgggatt tccggctcgt cgatccagat aatcgcgggc gtgtcgattt
     7441 cggcgagcgg tcacgcttgc tcgaggccgt agttcgcgag agcgacaaag atctggcgtg
     7501 tggcgcgcgc gattttgtgg aacattggcc tgacggacga atcaaattgc atgtgatccg
     7561 gcagactctc gcgtatcggc gcaagtacga tgaactgttt tccaaaggcg acttcattcc
     7621 tttggaggcg cgtggaaatc gcgcagaaca tgtggtggcc tatctacgaa gtttgggcga
     7681 gctctgcgct cttaccgtgg tgccaagatg gcttgctgcc actcaggccg agcctcctca
     7741 aatggatctt cgcgaatttt ggcaagatac cgctctccta cttccacgcg agacttctcg
     7801 ttcgtggcga aacattttta cgcatcggga aattggcagc caacttttcg gcgacaacca
     7861 gactctgcta ttgggcgatc tgctcgccca atttccagtt gccgttctca cctctgcgga
     7921 tagcgagagt cctggcgtcc gcaactgagt tgtcacgcag gaggcgagag cgcgcttttg
     7981 ggttcccaag tgtcgtgctc gggcttcgtg atatcatccc gcggcggagc ggcacaggct
     8041 ttcaaattta aatatagggt cgtccactag ccgcgacgca agctgtcaga tacgctgaat
     8101 ctcttctcaa tcttagcctt agaggtactc gcccatggca atgattaccg gtggagaact
     8161 cgttgtacga actctgatcc aggcaaacgt gaaaaatgtc ttcggtcttc acggcgcgca
     8221 tctcgaaacg atttttcaat cgtgcctcga tcatcgtatt ccgattaccg atacgcggca
     8281 cgaggtcgca gccggccatg ccgccgaagg ctatgcacgc tccacgcgca gcttgggagt
     8341 cgccatggcc acggcgggac ctggctttac gaatatcatc acctcgatcg ccaacgctta
     8401 cctggatcgc acgccggtgc tctatatcac cggatcgagc ggtctcgccg aggctgaaac
     8461 gaatactttg caatctggcc tcgaccaagt cgccgtggtt acgcccatca caaaatgggc
     8521 gcaccgaatt tcggtgacac agcagattcc ccgtcttgtc gcgcaagcca ttcgcttggc
     8581 gacatctgag cccatgggtc ccgttctatt ggacatgccc atggatgttt tgggcgcgca
     8641 ggttgaggag gcttccgtcc agattcccga aaaagtgtta gtggatgccc gctcggaacc
     8701 gcaatccagc gctatcgacg aagcgctaaa gctgctggcc agcgccgcgc ggcccgtgat
     8761 catggttggg gttggcgcgt accagtcagg cgcggaagaa gagctgcgcg cgtttgcgga
     8821 agccaccggc accccagtct tttcggactt tcaagcacat gggatgcttc ccagcaatca
     8881 tccactctat ggcggcacat ttcacaaaat ggccgacctt tccgcgccgg atcagcggcc
     8941 cgatctcgtg cttgcactcg gagttcgctt cggccttttc actttaggaa tgagtgattt
     9001 ggttgttcca cttgcggcga agatcgttca tgtggaaatc gacccgaaag aaatcggccg
     9061 cttgcgtcag gtcgccgtgg gcatcgtcgc cgactcgcgc gagactctgc acgcaatgaa
     9121 cgcgcgcgcg aagtctcaga agtggccgga tcgcagccgg tggcagcaga gtatccgcaa
     9181 cgccaaagtg gaacgatgca agcgcgcgca agccgacctg aaccgaacca caacaccgat
     9241 tcacccgctg caggcggtat cggcaattgt cgataacatt ccggagaata cgatcgtggt
     9301 cggcgatggc gcagaggcct atcactggat gaatgaggtg atccgccaga attctccggg
     9361 aagctacatc acgcacggat ttttgggagc ggtgggcatg ggactcggcc tatcgatagg
     9421 cgtgcaggtt gctaatcccg atcgccctgt actatgcctc gtcggcgatg gggctatagg
     9481 ttttacgatc gcggagtttg acacgatgat gcgccaccag ttgcctatcg ttgtcgcggt
     9541 gatgaacaat cacagttggg ctgcgtcaca gcactttcag gaaattgtgt ctggtaaaga
     9601 tcgactgacg ggcacggaac ttcgcgacgc gaagtatcat gaagtcgcgg cggcgttcgg
     9661 atgccacgcc caacgcgtaa ctgagctaca ggagcttggc ccagctatca aggccgcgtt
     9721 cgcgagcggc aagccggcat gcgtcaacgt ggcaattgac gtagttccac tgccacccga
     9781 acttcatctg ctcatggcgc gtcactgagc gagccgccgc caatgcaagt ccgtcgttta
     9841 ggaaaaaacg gccctgaagt ctccgcgatc ggtctcggct gcatgggcat gtccgggacg
     9901 tacggagcgg cagacgagac ggaaagcatc gcaacgatcc atgccgcgat cgaggcggga
     9961 gtcaccctat ttgacacggg cgatttttac ggcatgggac ataatgagat gctgcttcaa
    10021 aaagctctgg caggccgccc ccgtgaccgc ttcctcatcg gagtgaagtt cgacgggatg
    10081 cgcggcccga acggagcgtt catcggattg gatgcgcggc ccgtcgccgt gaagaacttc
    10141 ctcgcgtaca gcttgcgccg cctgggaacc gattacgtgg acttgtacca gccggcgcga
    10201 gtggatcctt cggttccaat cgaagacaca gttggcgcga tttcggaaat ggtgaaggcc
    10261 ggctacgtgc gccatatcgg gctttcagag gcgtccgccg acacgatccg ccgcgcgaac
    10321 gcgattcatc ccatcgcagc actgcaaatc gagtattcac tggtttcgcg cagcgttgaa
    10381 gcggagatcc tgccggcgct gcgcgaactg ggcatcagcc tggttgcgta tggcgttttc
    10441 tcg
//
LOCUS       DATNDT010000021         6310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3979891, whole genome shotgun sequence.
ACCESSION   DATNDT010000021 DATNDT010000000
VERSION     DATNDT010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6310
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3979891"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..128
                     /locus_tag="VN788_00795"
     CDS             <1..128
                     /locus_tag="VN788_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002768740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HXU19099.1"
                     /translation="VERVFAWLHNFRRLVTRWEYHVENFLGMVQLGCLKILLRHL"
     gene            173..1408
                     /locus_tag="VN788_00800"
     CDS             173..1408
                     /locus_tag="VN788_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU19100.1"
                     /translation="MKEARKGMWENIRRRLASIEPPIDADATELGPILEEIEARLAEY
                     IAPGGEGRSTRLFVSQLTREHLRKTIAFFLTMCPGEAAVPFQQAGSGTLNTLLLALLT
                     FIAELKKDNVIFAMEEPEIALPPHTQRRIANYLLEETSQCFVTSHSPYVIERFEPDGI
                     LKLNRDEHGILSGTAIKLPPGMKAKNYRQNFRRAIAEAMLGNGVIVGEGVTEQDALMV
                     VAQKMEEDNADLFPLDVAGLCVINAEGDGNLEKLGAFFREIGTPAFAFFDRKKRTDAE
                     IAALRSSYVVAMEIPQKGAEVLIAENTPLDRQWQYLEALRADDSDGRFMIPVARPVDD
                     VIRGLAVSTLKGLKGEGGAAKLLEFCAVGELPKIITDFLADIYQRFPKPKRKAIATTQ
                     PEAGADVAGDKAAAAAPGH"
     gene            1412..3145
                     /locus_tag="VN788_00805"
     CDS             1412..3145
                     /locus_tag="VN788_00805"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013698441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXU19101.1"
                     /translation="MALVICPRRQSILNEDGAVLVTGGPGSGKTTIALAKAKLIIERG
                     LSPGQAVLFLSFSRAAVARVVEASKTQLPATMRGNLSIQTFHSFFWRIVRAYGYLLGT
                     SRSLRLLLPHDEAAMRHECESTGGDWNVERERLFREAGRVAFDLFAPKAHELLSRSTR
                     LRELFSSCHPLIVVDEAQDTAEDQWQCVRLLSVGTQMMCLADLDQQIYDFRPGVSSER
                     VTHIMAALQPLRVDLAGENHRSPDSEIVAFANDVLLNTPRGAPYRGVSRMNYSANRDL
                     RDRAIRQATGIVIQRAKEAAHGNIETIAIMATWGRGVNVISRALTGDGTNLIPHRVMI
                     DEASVLLSSRMVAFMLEPRRPDTEEMLDLADGLDLTANVFRARGRATNLATARRLAMS
                     AVQCRAGTVPRANGVAAKLLATLRELRIHEFTGDPRRDWLDARSVLRNAGANPLQVIA
                     EDTEQLAAFQRGRRISAALTDLWQTQGEYRNARSAFDAAIAEDQLLSGGNDLRGIHVM
                     TIHKSKGKEFDAAVIFDDPNSSPLLFTQEGPPHSRCRKLARVGITRARHHVLMLCNLY
                     KPSALLEGHNL"
     gene            complement(3259..4191)
                     /locus_tag="VN788_00810"
     CDS             complement(3259..4191)
                     /locus_tag="VN788_00810"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXU19102.1"
                     /translation="MFPGVDHRLLRYVVAVSEELHFRRAAEKLHVAQPPLSRQIRDLE
                     NQLGVRLLDRTNQVVRLTEAGAVFVREAKQALLHGERAVNLAKAITQPSVFSLGYSPY
                     VSHEFVASIRALFASQFKNVRLSLVSAFADEELEQLQAGAPDAGLVTLPVAASALAVQ
                     RVLMEPLWVAISQKHRLGRVRTIRLRELLHLPMIAVAKRLCPQLHDRIHQAFARENSK
                     PQIGQEVTTAAEAISIIVGGLGFAFVRECDHQFRCPGVAFKRIAGEPLILESGIVYQP
                     DGGSPIIHALIAALQQRKEPSTVARNSAAAGLTA"
     gene            4505..4732
                     /locus_tag="VN788_00815"
     CDS             4505..4732
                     /locus_tag="VN788_00815"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU19103.1"
                     /translation="MPASLRNSAPSLPAAVSPTFERLLDSDEAAALLKIHPKTLQRMA
                     RQGQVAAVHIGKLWRFRASALDQWLNEKLAS"
     gene            4824..5990
                     /locus_tag="VN788_00820"
     CDS             4824..5990
                     /locus_tag="VN788_00820"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXU19104.1"
                     /translation="MFSRTRYQFGSLRLMERQRGPAAWEFRYYVDEGDGPGRRKRRHT
                     TIGTLEQYPTEATARKAVESLLLDLNAETPRAAFTVPTFGALVDKFVAEEMPERYSTR
                     KSYESMLKMHIRPRWADYPLDRLKPMVVEKWFRDLELAPKTKAHIRSLMHVIFKCAER
                     WDLVEMGKNPITLVRVKNSSKRLKRPRILTVEQFTSMLPHLPEPYRTMVVIAQCLGLR
                     VSEIMGLQWGDFDFKERTLLVERSVVHGQVDEVKTEYSRDHVPLDSRLAESVLAWRSL
                     APAAGPDDWVFTSPMTGRPYHQDMIQKRHLTRAAVRAGIGNGVGWHTFRHTYRSWLDE
                     TGAPMKVQQELMRHASIQTTMNVYGQAMTESKRTANSKVVEIVLGPEKLAASSA"
     gene            complement(6078..6154)
                     /locus_tag="VN788_00825"
     tRNA            complement(6078..6154)
                     /locus_tag="VN788_00825"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6118..6120),aa:Met,seq:cat)
     gene            6118..>6310
                     /locus_tag="VN788_00830"
     CDS             6118..>6310
                     /locus_tag="VN788_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19105.1"
                     /translation="MSPTSYRTAPPRTKMISWAAGNCKNGGAGRGEARLVHTRSMSSA
                     PGGALAIGDYGLVADGLTAA"
BASE COUNT         1292 a         1882 c         1947 g         1189 t
ORIGIN      
        1 tggtcgagcg ggtgttcgcc tggctccaca acttccggcg gctggtaacg cggtgggaat
       61 accacgtcga gaatttcctc ggcatggtcc agcttggatg cctcaaaatc ctgttgcggc
      121 atttatgaga tggcttctag aacgcggcac gctgctcgac aacatcctgc gcatgaaaga
      181 ggcgcgcaag gggatgtggg agaacattcg ccggcgcctg gcctcaatcg agccgcctat
      241 agacgcagat gccaccgagt tggggccaat cctcgaagaa attgaggcac ggcttgcgga
      301 gtacattgca cctggtggtg agggccgctc gacccggctc ttcgtctctc agctgacgcg
      361 cgaacacctg cgaaaaacca tcgctttttt cttgacgatg tgccccggtg aggcagccgt
      421 gcctttccag caggccggca gcggaacgtt gaatacgctg ctgttggcat tgctaacctt
      481 catcgcggaa cttaagaagg acaacgtcat cttcgcgatg gaagaaccag agatcgccct
      541 tccgccgcat acgcagcgac ggattgcgaa ctacctgctg gaagaaacgt cgcaatgctt
      601 cgtcacgtcg cactcaccct acgtcatcga acgtttcgag cctgacggga tactgaagtt
      661 gaaccgcgac gaacacggca ttcttagcgg cacagcgatc aagctgcccc ccggcatgaa
      721 agccaagaac taccggcaaa acttccgccg agccattgcc gaagccatgc tcggcaatgg
      781 cgtcatcgta ggggaaggcg ttaccgaaca ggacgcgctg atggttgttg ctcagaagat
      841 ggaggaagac aacgccgact tgttcccgct cgacgtggcc ggactctgtg tgatcaatgc
      901 cgaaggcgac gggaacctgg agaagctcgg ggccttcttc agggagatcg gaacgcccgc
      961 ctttgccttc ttcgaccgca agaagcgcac ggacgcggag attgctgccc tgcgaagtag
     1021 ctacgtcgtc gccatggaga tcccgcagaa aggcgccgag gtcctgatag cagagaacac
     1081 gccgctggat cgccaatggc aatatttgga agcgctgcgc gccgacgatt ctgacggtcg
     1141 attcatgatt cccgttgccc gcccagtgga cgacgtgatc cgcgggttgg ccgtttccac
     1201 gctgaagggt ctgaaaggcg aaggtggcgc tgccaagctg ctggaattct gtgctgtggg
     1261 cgagctgcca aagattatta ccgacttcct ggccgacatc tatcaacgtt tcccgaaacc
     1321 gaagcggaag gccattgcca ctacacaacc cgaggccggc gccgatgttg cgggcgacaa
     1381 agcggcggcc gcggcccctg gtcactgaaa catggccctg gttatctgcc cgcggcgaca
     1441 gagtatcctc aatgaagatg gagccgtcct cgtcacaggt ggccccggca gcggcaagac
     1501 aactatcgcc ctggccaagg ccaagctcat catcgagcgc gggctttcac ccggtcaagc
     1561 ggtcctattt ctcagcttct cgcgcgcggc ggtcgcgcga gtcgtcgagg cgagcaagac
     1621 gcagttgccc gccaccatgc gtggcaacct gtcgattcaa acgtttcact cgttcttctg
     1681 gcgcatcgtg cgggcgtacg gatacctgct gggtacatcg agatcgctgc ggctgctttt
     1741 gcctcacgat gaggcagcaa tgcggcacga gtgcgagagc acgggcggcg attggaacgt
     1801 cgagcgagaa cggctattcc gcgaggcggg cagagtcgca ttcgacttgt ttgcccccaa
     1861 agcccatgag ctgctatccc gcagcactcg gctgcgggag cttttctcat catgccatcc
     1921 gctgattgtc gtagacgagg cgcaggacac cgcggaagat caatggcagt gcgtccggtt
     1981 gttatctgtc ggcacgcaaa tgatgtgcct cgccgatctc gaccagcaaa tttacgactt
     2041 ccggcccggc gtcagttcgg agcgggttac gcacatcatg gccgccctgc agcctctgcg
     2101 cgtggacctt gcgggtgaga accaccgtag ccccgactcc gagattgtcg ccttcgccaa
     2161 cgatgtgcta ctcaacacgc cgcgaggtgc cccctaccgt ggcgtgtcgc gcatgaacta
     2221 tagcgccaac cgggatttgc gtgaccgggc gatccgtcag gcgaccggga tcgtgatcca
     2281 gcgcgcgaaa gaggccgccc acggcaatat cgagaccatc gccataatgg cgacgtgggg
     2341 acggggcgtc aatgtcattt cgcgcgctct gaccggcgac ggcacgaact tgataccgca
     2401 tcgtgtgatg atcgacgagg cttcggtgct cctgtcaagc cgcatggtcg cattcatgct
     2461 ggaaccgcgc agaccagaca cggaagagat gctggacctg gccgacggcc ttgacctaac
     2521 ggcaaacgtt tttcgagcac gcggtagggc cacgaacctt gccaccgcac ggcggcttgc
     2581 gatgagtgcg gtacagtgcc gcgcaggtac cgtgcctcgc gccaacgggg tggccgctaa
     2641 gctcctggcc acgctgcgcg aattgcgaat ccatgagttc acgggagacc ctcgccgcga
     2701 ttggctggac gcgcgcagtg tcctacggaa tgcaggcgcc aacccgcttc aagtcatagc
     2761 ggaagacacc gagcaacttg ccgcctttca gcgtggacgc cgcatttccg cagcgctgac
     2821 cgatctctgg caaacacagg gcgagtatcg gaatgcgcgc agtgctttcg atgccgccat
     2881 cgctgaagat cagctcttgt cgggtggaaa tgacctgaga ggcatccacg tcatgaccat
     2941 ccacaaatcg aagggcaagg aattcgatgc ggcggtcatc tttgatgatc ctaacagcag
     3001 cccgctgctg ttcactcaag aaggaccgcc tcattctcgt tgccggaagc tggcccgggt
     3061 tggtatcact cgtgcccgac atcacgtcct gatgctctgc aatttgtaca aaccctccgc
     3121 cctgctggag ggccataacc tttaagcctg cggttctagc tagaagacgc ccgcgcgcgc
     3181 cctgattccg cctgacctgg ccagtcttgg ggagaccacc tgagatgtgg cggaagttgc
     3241 agcgcagacc gcagttgttc acgccgtcag gccggccgcc gctgagttcc gggcaacggt
     3301 gctgggttct ttccgctgct gaagagcggc gatcagggca tgaatgattg gtgagccgcc
     3361 gtctggttgg tatacgatcc cgctttccag gatcagcggc tcacccgcga tgcgcttgaa
     3421 ggcaactccg gggcatcgga actggtgatc gcattcccgc acaaacgcga atcccagccc
     3481 gccaacaatg atcgagattg cctctgcggc cgtcgtgacc tcctggccaa tctgcggttt
     3541 ggagttctcg cgggcaaacg cctgatgtat ccggtcgtga agttgggggc aaagccgctt
     3601 ggcgacggcg atcatcggca agtgaagcaa ctcccgcagc cggatcgtcc gcacccgccc
     3661 cagccggtgt ttctgtgaga tcgcgaccca taaaggctcc atcagcacgc gctgaacagc
     3721 gagagcgctt gcggcaacag gaagggtgac gaggccagca tccggcgccc cagcctgaag
     3781 ctgttcaagc tcttcatctg cgaaagcact gacgagcgag agccggacgt ttttgaactg
     3841 cgacgcgaac agcgcccgga tcgaggcgac gaattcgtgg ctcacatacg gcgaatatcc
     3901 tagcgaaaac acgcttggct gtgtgatggc cttggctagg ttcaccgcgc gctcgccatg
     3961 caagagcgcc tgctttgcct cacgaacaaa aacggccccc gcttcggtta ggcgcacgac
     4021 ttggttggtg cgatcaagca accggacgcc gagttggttc tccaaatccc tgatttgccg
     4081 gctgaggggc ggttgagcga catggagctt ttccgctgca cgccgaaagt gcaattcctc
     4141 ggagaccgcc acaacgtagc gcagcaggcg gtggtcgacg ccaggaaaca tgcttgactc
     4201 ctcttctcga attcgcggcg caggcgtggt acagcgtcat ccacaccacc caacctgctg
     4261 ggccctgcac actgaggcat acgagaagcg gcgtctgaac tcatgatatg cctcccgcgc
     4321 gaatcgttcc agagttggtt aaggtggcat cgagtggagc ctcttggcac cctcaaaaat
     4381 cgcgccttag gcggccactt tgataccggc caggtatcag gcgataccga caaagtattg
     4441 gaccgcgccg cgctggccgc catagcctga atttgctctg gaaaaacgtc ggcaggagta
     4501 ccccatgccc gcctcccttc gcaattcggc accgtcgctt cccgcggcag tttccccaac
     4561 cttcgagcgt cttctcgatt cagacgaggc tgcggcgctg ctcaaaatcc atcccaagac
     4621 gcttcagcgc atggcgcgcc aaggtcaggt ggcagccgtt catattggga agctctggcg
     4681 cttccgcgcc tctgcgctcg atcagtggct caacgaaaaa ctcgcgagct aaggcgacgc
     4741 gccgatggac tcccggcggc gaatgcaggt aggctggcaa cagccacctg tgcccgttga
     4801 aacggaaagg agttgttgcc tcgatgtttt cacggacacg gtatcagttc ggtagtttgc
     4861 gattgatgga gaggcagcga ggtcccgccg cttgggagtt ccgttactac gttgacgaag
     4921 gggatgggcc aggccgccga aagcggcggc acaccaccat cgggacgttg gagcagtacc
     4981 ccacggaggc gacggcacga aaggccgtag agtccctgct gcttgattta aacgctgaaa
     5041 ccccgcgcgc cgcgttcacg gtgccgacgt tcggagcctt ggtggacaag tttgtcgccg
     5101 aggagatgcc ggagcggtac tcgacgcgga agtcgtatga atccatgctg aaaatgcaca
     5161 tacggccgcg ctgggcggat tatcccttgg accggctcaa gcccatggtc gtggagaagt
     5221 ggttccgcga tctggaattg gcgccgaaaa ccaaggcgca tatacgaagc ctcatgcacg
     5281 tcatcttcaa gtgcgccgag agatgggacc tagtggagat gggcaagaac ccaatcaccc
     5341 tggtgcgcgt gaagaacagc tcgaagcggt tgaagcgccc gcggattctc acggtcgagc
     5401 agttcacttc catgctgccg cacctgccgg aaccgtatcg aaccatggtc gtcatcgccc
     5461 aatgcctcgg gttgagggtg agcgagatca tggggttgca atggggcgat ttcgacttca
     5521 aagagcgaac gcttctggtc gagcgaagcg tggttcacgg gcaagtggac gaggtgaaaa
     5581 ccgagtactc ccgcgatcat gtgccgctcg attcgcgctt ggcggaatct gttctggcat
     5641 ggcggagcct ggctccggca gccggcccgg atgactgggt gttcaccagc ccaatgaccg
     5701 gacggcccta ccaccaggac atgattcaga agcgccactt aacgcgggcc gcagttcggg
     5761 ccggcattgg caacggtgtc ggctggcata cgtttcgcca tacctaccgc tcgtggctgg
     5821 acgagaccgg cgcacccatg aaggttcagc aagagctgat gcgccacgcc tcgattcaga
     5881 ccacgatgaa cgtgtacggg caggcgatga cggaaagcaa acggacggcg aacagcaagg
     5941 tagtggaaat cgtgctggga ccggaaaagc tcgccgcaag cagcgcttaa gggcgccctt
     6001 ttggcggcgt ttttcttaat gggggttttt gggggtttgc aggagaggct caaaatcccg
     6061 caactgattt gttttgttgg ttgcgggggg tggatttgaa ccaccgacct ttgggttatg
     6121 agcccaacga gctaccggac tgctccaccc cgcaccaaga tgatctcatg ggcggcgggg
     6181 aattgcaaaa acgggggcgc gggccggggc gaagcgcggc tggttcacac tagatccatg
     6241 agctcggcac cgggcggggc gctcgcgatc ggcgactacg ggctggtggc cgacgggctg
     6301 accgcggccc
//
LOCUS       DATNDT010000022         4452 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2257157, whole genome shotgun sequence.
ACCESSION   DATNDT010000022 DATNDT010000000
VERSION     DATNDT010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4452
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2257157"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..104)
                     /locus_tag="VN788_00835"
     CDS             complement(<1..104)
                     /locus_tag="VN788_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXU19106.1"
                     /translation="MGPVGVQEMLVIFIVALVLFGPKKLPELGKTLGKA"
     gene            189..1139
                     /locus_tag="VN788_00840"
     CDS             189..1139
                     /locus_tag="VN788_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmoA family protein"
                     /protein_id="HXU19107.1"
                     /translation="MTSRLWRHFILAASLTACFPLLGQVQLKPVSDGIAIDIGGKPFS
                     SLHMGPDAPKPYLHPIRSASGKEVTRGFPMETVEGESHDHPHQRGLWFAHEDVNGFDF
                     WNNEFSYTTKNRGFIVLDRLVSAQSGQKSGTIHAIFDWKDPHGKILVKEDRTMTFYDD
                     PSLRIMDFDITLTAVTDCVFGDAKDGTFGIRIADVMNEKHTGKITDSEGRVGMKEVWG
                     KRADWVDYVGTIAGEKLGIAIFDHPANPGHPNRWHARDYGLFALNPFGDHVFDKSAPI
                     TPHPLPAGKSWRYRWRVIIHPGDTQSAGIAGMYKKYAASQ"
     gene            complement(1133..1573)
                     /locus_tag="VN788_00845"
     CDS             complement(1133..1573)
                     /locus_tag="VN788_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19108.1"
                     /translation="MLTYTGTVNFDREIDSVALPAHLAACFRRQMDARTIEIASNRVT
                     FRGSVFAAPFRFVELTVDCAACAVHYRLSLRWLILAAIWLALAPAVVIAGPPVAGDPP
                     KLVALTWMWFGGTILFVAIQLVTAIPRFQCLVRRAIASAPAKPH"
     gene            complement(1621..2442)
                     /locus_tag="VN788_00850"
     CDS             complement(1621..2442)
                     /locus_tag="VN788_00850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HXU19109.1"
                     /translation="MSDTGNVQPIIIRKKKAAHDGHHGGAWKVAYADFVTAMMSLFIV
                     LWLMSSSEPIKKAIAGYFHDPNGVEKMMGSDMAGTGESLSLKKADMNQLKEKLNDAFK
                     QLPEFKEIKEHVQMTVTGEGLRIELLETAKGMFFENGNANPSPDGKELLLSLAQELAK
                     LPNKITIEGHTDAKPYASLTYTNWELSADRANAARRLMQADGLRDNQVTEVRGFASQR
                     LRKPDDPTDPSNRRISVIVQYARGGNPAAIPAAIKEAVAPATPAKPSPAHGLPAR"
     gene            complement(2449..3321)
                     /gene="motA"
                     /locus_tag="VN788_00855"
     CDS             complement(2449..3321)
                     /gene="motA"
                     /locus_tag="VN788_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HXU19110.1"
                     /translation="MFAIIGFVVVIGAVIGGFLMEHGKLLVLMQPSELVIIGGAGIGT
                     LLIANPVPVLIRLLKGAIGILQPSRHAKPFYIETLKMLNELFQQARKGGIAKLESDVE
                     EPEKSPIFSKYPKFLKDHHAVDFVCDTLRMAVTGGVTHFDLDQMMESDMEVHHHEVLV
                     PINALTTMADSLPGLGIVAAVLGVVITMGALGGPPEEIGHKVAAALVGTFLGILMCYG
                     FFGPLASNMSKASESESHYYHCLRVAIIAFVRGAPPILAVEFARRVIPAEHRPSFKEM
                     EAGVKGGGTVAAAA"
     gene            complement(3388..3615)
                     /locus_tag="VN788_00860"
     CDS             complement(3388..3615)
                     /locus_tag="VN788_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19111.1"
                     /translation="MTKVQLPYALRRSITDKDAKGISDVHGAYGIQRVKLGSGMDAIT
                     VEYDASRLKERDVEATLIRYGIPIERRWNVA"
     gene            complement(3630..>4452)
                     /locus_tag="VN788_00865"
     CDS             complement(3630..>4452)
                     /locus_tag="VN788_00865"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HXU19112.1"
                     /translation="SMAGQKLDVHPEAGRAPQTIPVGDTARVIDEPTFQKAVSENPGF
                     LKDWKGAITAGASLVQATQTSRSFTGSVNLIRAIPSETWLNPRNRTTFNFTAAYGVLD
                     QPKTAEIKTEIYHADAERDEYFSPRLYAFGQLAYDHNVSQGLTLQQLYGGGVGWTALK
                     KANQELDLKASLSYIEQSFTDAGKEQSLFGSTFAEDYNRKFIHGIVLTEQLAITPAWT
                     NTDAYSALGNLVLTMPVYKRLNITFGTVDTFLNDPPPGFKKNSFQLTTGLAYMLQ"
BASE COUNT          929 a         1275 c         1313 g          935 t
ORIGIN      
        1 gctttcccga gtgtttttcc gagctcggga agtttcttcg gaccaaataa caccagggcc
       61 acgataaaga tgacgagcat ttcctggacg ccgacgggac ccatggataa acctccagac
      121 gcttacatca taacaccgcg atcgttcgcg gccccgcatc cggtcgaatt gcattagact
      181 aagcccaaat gacttcccgg ctgtggcgtc acttcattct cgcggcctcg ctcaccgcgt
      241 gttttccgtt attagggcaa gtacaactca aaccggtatc cgatgggatt gcgattgata
      301 ttggcggcaa gcctttctcg tcgctgcata tgggaccgga cgcgcccaag ccctaccttc
      361 acccgatccg gtcggcatcc ggcaaggagg ttactcgggg gttcccgatg gagacggtgg
      421 agggcgaatc ccacgatcat ccgcaccagc gaggcctctg gttcgcccac gaggacgtga
      481 atgggttcga tttctggaac aacgaattca gttacacgac caagaaccgc gggttcattg
      541 tgctcgaccg gctggtcagc gcccaaagcg gccagaaatc cgggacgatc cacgcgatct
      601 tcgattggaa ggatccgcat gggaagatcc tggttaaaga ggatcgcacg atgacttttt
      661 acgacgatcc gtcgctccgg atcatggatt tcgatatcac gctgactgct gtaaccgatt
      721 gcgtctttgg cgacgcgaag gacggcacat tcgggattcg catcgccgac gtaatgaatg
      781 agaagcacac cggaaagatt accgactcgg aggggcgggt ggggatgaag gaagtctggg
      841 gcaagcgcgc ggactgggtg gattatgtgg gaacgatagc aggcgaaaaa ctcggaatcg
      901 cgattttcga tcacccggcg aatccaggcc acccgaatcg ttggcacgct cgcgactacg
      961 gcctgttcgc gctcaatccg tttggcgacc acgtcttcga caaatccgcg ccgattacgc
     1021 ctcacccgct gccggcgggt aaaagctggc ggtaccggtg gcgggtgatc attcaccctg
     1081 gcgacacgca atcggcgggg atcgcgggga tgtacaaaaa atatgcggca agtcagtgag
     1141 gcttcgcggg ggcgctcgcg atggcgcgcc ggacaagaca ctggaaacgc ggaatcgcgg
     1201 tgactagctg aatcgcaacg aacaagatag taccgccaaa ccacatccaa gttaacgcga
     1261 ctaacttcgg cggatccccg gcaaccggcg gacccgcgat gacgactgcg ggcgcgaggg
     1321 cgagccaaat tgccgccagg atcagccacc ggaggctcag ccggtagtgg accgcgcatg
     1381 ctgcgcaatc caccgtcagc tccacaaacc tgaatggtgc ggcgaaaacg ctgccccgga
     1441 aggtcacccg attgctcgcg atctcgatcg tgcgcgcgtc catctggcgg cgaaagcagg
     1501 cggcaaggtg agcgggcaat gcaaccgaat caatctccct gtcgaaattc accgttccgg
     1561 tataggtgag catcaggcgc agtgcgcttg ttcaagatag cgcgcttgcg agccaatctg
     1621 ctaccgagcg ggcagtccgt gagccgggga gggtttcgcg ggcgttgcgg gggcgacagc
     1681 ttccttgatg gcggccggga tcgcggccgg attgccgccc cttgcatatt gcacgatcac
     1741 gctgatgcgg cggttcgacg ggtcggtggg atcgtccggt ttacgaagtc gctgggaggc
     1801 gaagccccgc acctcggtga cctggttgtc ccgtaggccg tcggcctgca tcaagcgccg
     1861 ggcggcattc gcgcggtccg ctgaaagctc ccaattggtg taggtaaggc tcgcgtaggg
     1921 cttcgcatcc gtgtggccct cgatggtgat cttgttgggc agcttggcca gttcttgagc
     1981 cagactgagc agcagttcct tgccgtcggg actcgggttg gcgttcccgt tctcgaaaaa
     2041 cattcccttc gcggtctcga gcagctcgat gcgcaaaccc tcgcccgtga cagtcatctg
     2101 cacatgttct ttgatctcct tgaactcggg aagttgcttg aacgcgtcat tcagcttctc
     2161 cttgagctgg ttcatgtcgg ccttcttgag ggagagagac tcgcccgtac cggccatatc
     2221 gctacccatc attttttcga ctccgttggg atcgtgaaaa tatccggcga tggctttctt
     2281 gatgggctcg cttgagctca tcagccagag cacgatgaat aacgacatca tcgcggtgac
     2341 gaagtccgca taggccacct tccatgctcc gccatgatga ccgtcgtgag cggctttttt
     2401 cttgcggatg ataatcggtt ggacgttgcc ggtgtcggac atggagcttt acgcggcagc
     2461 ggcgacggtt ccaccgccct tcacgcctgc ctccatttcc ttgaagctgg ggcgatgctc
     2521 ggctggaatc acccgccgcg caaactcaac ggccaggatg ggcggcgcgc cgcgcacgaa
     2581 cgcgataatt gccacacgca aacaatggta atagtgagat tcggactcgc tcgccttgga
     2641 catgttggac gccagcggcc cgaaaaaccc gtagcacatc aggatgccca ggaaagtgcc
     2701 cactagcgcc gcggcgactt tgtgcccgat ctcttcgggc ggccctccaa gcgcgcccat
     2761 cgtgatcaca acgcccagaa cggcggcaac gatgcccaat cccgggagcg aatcggccat
     2821 ggtcgtcaag gcattgatcg gcaccagcac ctcatgatga tgcacttcca tatcggactc
     2881 catcatctga tcgaggtcga agtgcgtaac accgccggtg actgccatgc gcagcgtgtc
     2941 gcagacgaaa tcgaccgcgt gatgatcttt gaggaatttc ggatatttcg aaaagatcgg
     3001 gcttttctcg ggctcctcca catccgattc caacttcgcg atgccgcctt tgcgggcttg
     3061 ctgaaacagc tcgttcagca tcttcaacgt ttcgatataa aacggtttgg catgccggct
     3121 gggctgcagg ataccgattg cgcctttcag cagccggatc agcaccggaa ccggattggc
     3181 gatcaggagg gttccaatgc cggcgccccc aatgatcacc aactcagacg gctgcatgag
     3241 cacgagcagc ttaccgtgct ccatgaggaa accaccaata acggccccaa ttacgacgac
     3301 gaatccaatg atggcgaaca taccgacgcg gcccctttta ggggctaatt gtctatatcg
     3361 gctgtccggc ggaaaaagtt taaggggcta agcgacattc catctgcgct cgatcgggat
     3421 gccatagcgg atcagcgtcg cttcgacatc ccgctccttc agcctggaag cgtcgtattc
     3481 cacggtaatc gcgtccatcc cggagccgag cttcacgcgt tgaatgccat aagcgccatg
     3541 aacatccgaa atgcccttgg catccttatc ggtgatggat cggcgtagcg cgtaggggag
     3601 ttgtaccttg gtcacggaat ttcagaatat cattgcagca tataggccag ccccgtcgtg
     3661 agttggaatg agttcttctt gaaacccgga ggcggatcgt tcaagaaagt atcgaccgtg
     3721 ccgaatgtaa tgttcaagcg cttataaacc ggcatcgtca gcaccagatt gccaagggcc
     3781 gaatacgcat cggtgttagt ccatgctggc gtgattgcga gttgctccgt gaggactatc
     3841 ccatggataa acttgcggtt atagtcctcg gcgaaggtag aaccgaacag gctctgttcc
     3901 tttccggcgt ccgtaaagct ctgctcgata tagcttaagc tggctttcag gtccaactcc
     3961 tgatttgctt ttttcagcgc cgtccaaccg acgcctccgc cgtaaagctg ctgaagggtg
     4021 aggccctgag agacattgtg gtcgtaagcg agttgtccaa acgcatagag gcgcggcgaa
     4081 aagtattcgt cacgctcggc gtccgcatga taaatttcgg tcttgatctc ggccgttttt
     4141 ggctgatcca aaacgccgta ggcggcggtg aagttgaagg tagtgcggtt gcgcgggtta
     4201 agccaagttt cggacggtat tgcgcgtatg agattgactg atccggtgaa gctgcggctg
     4261 gtttgcgtcg cctgaaccaa cgaagcgccg gcggtgatcg cgcctttcca gtccttgaga
     4321 aatccgggat tttcggacac ggctttctgg aaggttggct catcgatcac gcgcgccgta
     4381 tcgccgaccg gaatcgtctg gggcgctcgc ccggcctcgg gatgaacatc gagcttctgt
     4441 ccggccatgg ac
//
LOCUS       DATNDT010000023        10156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3977612, whole genome shotgun sequence.
ACCESSION   DATNDT010000023 DATNDT010000000
VERSION     DATNDT010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10156
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3977612"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VN788_00870"
     CDS             <1..807
                     /locus_tag="VN788_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03118 family protein"
                     /protein_id="HXU19113.1"
                     /translation="NGVSAFVISGINPITKMQASGNSIFVFAALDGTIWGWNPAVGGI
                     NPMTGASIADLGADRSTAGATYTGLAIANDNGKPLLYAADDGPNRRIDVFDSNFQLVT
                     DLGPDAFVDPKIPQKFAPYGIQTITGTDGSQVIWVTYTALDKAQSGFVDAFSPAGVLL
                     SHFALTGPLHSPWGVALAPADFGPMSNAILVTNNTPRGRINAFDPKTGAFLGPLRDTS
                     GKPIEIDDIWGIQFGQGSVAGSNGNPNQLFFTAGNNNYGSGTFGVITFGQ"
     gene            complement(1001..2767)
                     /locus_tag="VN788_00875"
     CDS             complement(1001..2767)
                     /locus_tag="VN788_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXU19114.1"
                     /translation="MAHLRLRTQFLFATLLIISLLTGTLLFILRRAVRSEIATQVQEA
                     TLASLRAFETVQREHQKELSQTAAILAELPTLKALMTTADAPTIQDGSEPFWSLAGSD
                     LFLLASPEGKILGFHAAAPGWPSGSAQGDLNESLKADESSAWWYADGQLYWVFLRSIT
                     AGTGAEQRRLGVLAVGYRVDAKVAQQLALFSGSQIALTASGRVVASTLPPQEEAQLQR
                     LIDRQGPPGDRTAHEIILHTDQYQFASALIREGPRTPVACYVLTSLKQANAFIRRLNK
                     IIFSLGAAALLLAGLLIGAVSRTIARPLEDLVAGAGALGHGDYAYSINPRGSSEVAGL
                     GEAFSTMRQELLIAQQRRLTAERIAALGRAAGSISHDLRHHLAAMVANAEFLYEADRL
                     KLDKDEIYDEIKTASNQMVDLLDSLRELAREGVAIAPVPASLAQTARRAVEAVLASPE
                     SRGRTVSLDVSGETDGVFDPKKVERALFNLLLNACEATTAGKGRIEVRVNSSTKAFEI
                     RVVDNGSGVPGSIRDTLFDPFVSSEKPNGTGLGLAIVNKIIHDHDGTVSLETTSDLGT
                     VFLVRLPRVRHVVAADAAPVPS"
     gene            complement(2788..3519)
                     /locus_tag="VN788_00880"
     CDS             complement(2788..3519)
                     /locus_tag="VN788_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU19115.1"
                     /translation="MDRVLVVEDSRPMQRTLQRLFGSDGLAVDIAPDGVAGLEMFLKR
                     IPSVVVLDLKLPRMPGKELCRAFKSRAASVPVVVLSADAQVEDKVLLLELGADDYVTK
                     PFSPKELLARVRRAMRRAGDNALPAKTAVGSEHELLTFGEVSIDFTSMEALRSGGKLT
                     LTTQEFKVLKFLASSPGTVISRAKLLNEVWGYENYPTTRTVDNHILRLRQKLEPDPRN
                     PRYFLTIHGAGYKFAPGASISVPGK"
     gene            complement(3545..4216)
                     /locus_tag="VN788_00885"
     CDS             complement(3545..4216)
                     /locus_tag="VN788_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19116.1"
                     /translation="MPVTADVEVVRPRVKSQHTRSVKIADASNVAVWLIPIDNVLSAT
                     PAGTSAKPIPQLVQRNKTFQPHILVVQVGTLVQFPNRDPFFHNIFSLFDGKRFDLGLY
                     EAGSSRAVRFDRAGVSYLFCNIHPEMSAVVIAVATPYFGLSDRTGHVTMDGVPVGRYE
                     IQVWYERSRQADLNASAQEVDISTAARSLGLLHVVEDPSFTEAHKDKYGRDYVPPPNP
                     GYLEK"
     gene            complement(4321..5760)
                     /locus_tag="VN788_00890"
     CDS             complement(4321..5760)
                     /locus_tag="VN788_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19117.1"
                     /translation="MSELRAEEQRDREESRSLRLELERENRSATSEHSNPGEIGPYAS
                     RGPTGDSSEPSTSSTTSGSAAQNQDIAERVAELEEDQQLARAELKEQSQTKVEAASKY
                     RVRLSGIALLSVFDNRGTVDDQEVPEIASPRQPFDSPGTFGASLRQSQVGIEAFGPDF
                     AGAHTSASLKFDFAGGFPNTPNGISMGLVRLRTGTIRLDWATTSVIAGQDGLFFAPLE
                     PTSLASLAVPPLSYAGNLWGWIPQVRIEHRIDLPKSSNLNVQAGILDSLSGDVPQSTF
                     ERSPTRGEKSGQPAYAARVAWNVPASGQTLTIGAGGYYGRQYWGFGQNVDGWAGTADL
                     TVPLGRLFALRGEFYRGRAMGGLNGGIGQDVLLSGPLANQGTIVRGLDSAGGWAQLKF
                     KPRANFEINGAFGEDSPFASELERFPATQGAYGILLSRNSSPFVNFIYQIRSDVLFSA
                     EYRRLQTSLLHNTASQTANHINLSLGYLF"
     gene            6319..7398
                     /locus_tag="VN788_00895"
     CDS             6319..7398
                     /locus_tag="VN788_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXU19118.1"
                     /translation="MKRGIVASAATVTGGILAAQLSAREQEDEKSGKLTKGDAAILRF
                     LAAAEILETDLWVQYNELGGVQDNEVPGGTGSMPYTVALQVLDSDMPQYIHDNTEDEF
                     THEDFINAYLASKGADIVDLERFRTLPGSTATGSSGKLRLTNLMELTVDTSWWTRYRS
                     RTKNPDFGDTFPQAIPSLAVGMHPAIPRTDADLTPGDHIQAIANTAGFHFGTIEQGGT
                     SLYPSLAQRVTSVEVLRIVLSIGPTEAMHFQTWQDKAGNAPPLTDPSDPTLVFPDLNS
                     SPFGGEDFQTNLIMPEPTIFLSRSLPACSIVRPTETNGAATAVVAFLTAMGLFRGQSP
                     AFTAFLNDLAEDADDARRQGQAARD"
     gene            7573..8163
                     /locus_tag="VN788_00900"
     CDS             7573..8163
                     /locus_tag="VN788_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HXU19119.1"
                     /translation="MPTRDDAWTLLCEYTQSESLRKHALAVEACVRAYARKVGADEDL
                     WGTTALLHDFDYERWPNEEHHPSEGHPGGGARILRDHGYPEEMVRAILSHGDYTGVRR
                     ETPLEHTLFACDELAGFLTACALVKPGKSILEVEIDSVKRKLKDKAFARGVNRDDVRK
                     GAEELAIPLEEHIGFCIGAMRAAADALGLDGKASRT"
     gene            complement(8230..8769)
                     /locus_tag="VN788_00905"
     CDS             complement(8230..8769)
                     /locus_tag="VN788_00905"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXU19120.1"
                     /translation="MKAFKLSAALFTLFLLMSGCSNTPKSPDVSASVRKSLDQAGLKD
                     VSVSDDRDKGVVTLNGHVPSDADKQNAESIAKSAAGSQVVANQIEVTPKAAESEAKTI
                     NSDLDKGISKNLDAALVQHNLKKGVSCEVKNGVVTLSGTVSSESRRTEVANIASKVPN
                     VSQVVNELQIKGQKATSSQ"
     gene            complement(8822..8983)
                     /locus_tag="VN788_00910"
     CDS             complement(8822..8983)
                     /locus_tag="VN788_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 domain-containing protein"
                     /protein_id="HXU19121.1"
                     /translation="MLLLIVLLVLLFGGGGGYYGYTRWGAGGGLGILGTVLLIVLVLY
                     LLGGLGLSR"
     gene            complement(9352..9708)
                     /locus_tag="VN788_00915"
     CDS             complement(9352..9708)
                     /locus_tag="VN788_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXU19122.1"
                     /translation="MTKRVPLVIRCPLVLAAATLISLAMLAEGCNTTEPPNRQLSDAQ
                     ITTRVKAKLATEVGGSSLTNVEVSTTNGVVTLAGQVQSADVKKKSETVSASVPGVVRV
                     NNNLQVQSPSGSAKAP"
BASE COUNT         2240 a         2952 c         2799 g         2165 t
ORIGIN      
        1 aacggcgtaa gcgcttttgt catctcggga atcaacccga tcacgaaaat gcaagcttcc
       61 ggaaactcca tttttgtctt cgctgcttta gacggcacaa tttggggttg gaatcccgca
      121 gttgggggca taaatcccat gacgggtgcc agcattgctg atcttggcgc tgacagatca
      181 accgccggcg ccacttacac gggtctggct attgcaaatg acaatgggaa accactgcta
      241 tacgctgccg acgacggacc aaaccgcagg atcgacgtct ttgactccaa tttccagttg
      301 gtgaccgatc ttggtccgga tgcgtttgtc gatcccaaaa ttccgcagaa attcgctccc
      361 tacggcattc agacgatcac cggcacggac ggctcgcaag tgatttgggt cacctatact
      421 gccctcgaca aagcgcagag tggtttcgtg gacgcgttta gcccagcggg cgttctgctg
      481 tctcactttg cgttgactgg ccctctgcat tcaccgtggg gcgtcgcatt ggctcctgcc
      541 gatttcgggc caatgagcaa tgcgattctc gtcacgaata acactccccg agggcggatc
      601 aatgcctttg atccaaaaac cggcgccttc ctcgggccgc tgcgtgatac cagcgggaag
      661 cccatcgaga tcgacgatat ctggggcatt cagtttggcc agggcagcgt ggcgggctcc
      721 aacggcaacc ccaatcaact cttcttcacc gctggcaaca acaactacgg cagtggtacg
      781 ttcggcgtca tcactttcgg ccagtaaaac ggatgggtgg gacaggggag caagtggccc
      841 ggagagagcg ttcctctctg ggcgcttgcg tttttaacag ctaacaggag cggccatgcg
      901 ctggcagtgg agttgggtct ttccgtggtc gagtcaaacg acctcagtgt gtgaccggtc
      961 gatagccaga ttgcagcgtc cagaaatcac gcgtcggtac tcaggaggga accggcgccg
     1021 cgtccgcagc cacgacgtgc cgtacccttg gcaacctgac aaggaacacg gttcccaaat
     1081 ccgacgttgt ttctaggctg accgtaccgt catgatcgtg gatgatcttg ttgacgatcg
     1141 cgagacccag tcctgttcca ttcggctttt ctgaacttac gaaagggtcg aagagagtat
     1201 cgcgtatcga gccaggcacg cctgatccat tgtcaaccac tcggatttcg aatgcttttg
     1261 tggacgaatt tacccggacc tcgatccgac cctttccggc cgtcgtggct tcgcatgcat
     1321 ttagaagcaa attaaagagt gcccgctcaa ctttcttagg gtcgaataca ccgtccgtct
     1381 cgccggagac gtcgagggag actgtccggc ctcgggattc tgggctggct aggacggctt
     1441 caaccgcacg gcgcgccgtc tgggcaaggc tcgcaggcac gggcgcaata gcgactccct
     1501 cccgggccaa ctcgcgcagg gagtccagca aatcgaccat ttgattcgac gccgtcttta
     1561 tctcgtcgta aatttcatcc ttgtcgagct tgagtctgtc cgcttcgtat aagaattccg
     1621 cattggcaac catcgctgcg agatggtggc gaagatcgtg cgagatcgag ccagccgccc
     1681 ggcccagagc ggcgattcgc tctgcggtca acctcctttg ttgggcgatc agcaattcct
     1741 gacgcattgt cgaaaaagcc tccccaagcc ccgcgacctc gctacttccg cgcggattga
     1801 tggagtaggc gtagtctcca tggcctagcg ctccggctcc ggccacaaga tcttctagcg
     1861 ggcgcgcgat ggtccgagat acagccccaa taagcaaacc agcgagcaac agcgccgcgg
     1921 cccccagaga aaagatgatc ttgttgagtc gtcgaatgaa agcgttcgct tgcttcagag
     1981 acgtgaggac atagcaggca accggcgttc gtggaccttc gcgtatcaac gctgacgcaa
     2041 actggtattg gtcggtatgt aggattatct cgtgagcagt tcggtcaccc ggaggcccct
     2101 gtcgatcaat gagccgctgc agttgcgcct cctcttgggg cggcagcgtg gacgcgacga
     2161 ctctgccact ggcggtcaac gcaatctgac ttcccgaaaa taatgcgagt tgctgagcca
     2221 ccttcgcatc cacgcggtat cccaccgcga gaaccccaag ccggcgctgc tccgctccgg
     2281 ttcctgctgt gatggaacgc aaaaaaaccc aatagagttg accatcagcg taccaccagg
     2341 ctgagctttc gtctgctttc agggactcgt tcaaatcacc ctgggcggag ccggagggcc
     2401 agccaggcgc tgccgcgtga aatcctagaa tttttccttc ggggcttgcc aagagaaata
     2461 gatcgctgcc cgcaaggctc cagaagggtt ctgacccatc ctgaatcgtg ggtgcgtcag
     2521 ccgtggtcat cagagctttg agggtaggaa gttccgctag tatggcggct gtttgggaga
     2581 gttccttctg atgctcgcgt tgcacagttt caaacgctcg tagcgaggcg agggtagctt
     2641 cttggacctg ggtcgcgatt tcggagcgga ccgcgcggcg caagatgaaa aggagcgtgc
     2701 ccgtcagcag gctaatgatc agaagcgtag caaataggaa ttgagttcgt agtcgtaggt
     2761 gagccatcgg acttctagcc ctcatggcta ctttcccgga acgcttatcg acgctcccgg
     2821 cgcgaacttg tagcccgctc cgtgtatcgt cagaaagtaa cgaggattgc ggggatccgg
     2881 ttccagtttc tgccgcaagc gaagaatgtg attgtcaacg gttcgggtcg tcggataatt
     2941 ctcatacccc cacacctcat tgagcagctt agcgcgcgag atcaccgtac caggcgaact
     3001 tgcaagaaac ttcagaacct tgaattcctg cgtcgtcagc gtcagctttc cccctgagcg
     3061 tagggcctcc atgctggtga aatcgatgct gacctctcca aaggtgagga gctcatgttc
     3121 gcttcccacc gcagtcttgg ctggcagcgc attgtctccc gctcggcgca tcgcccggcg
     3181 gactcgagcg agcaattcct tggggctgaa gggctttgtg acgtagtcat ccgccccaag
     3241 ctcaaggagc agcaccttgt cctccacctg tgcgtcggcg ctcagcacaa cgacaggcac
     3301 ggatgcggca cggctcttga acgcacggca cagttccttg ccgggcattc gtgggagttt
     3361 aaggtcaagt acaactacgc ttggaattcg cttcagaaac atctcaagac cagccacgcc
     3421 gtccggcgca atatcgaccg caagcccatc cgaaccgaac agacgttgca gggtcctctg
     3481 catcggccgg ctgtcttcta cgaccaacac tctatccatc ggtccgatct cccttcgggt
     3541 tcgctcactt ttctagatag cccgggttcg gcgggggaac gtaatcccgc ccgtacttgt
     3601 ccttgtgtgc ctcggtgaaa ctaggatctt caacgacatg cagcaaaccc aaggaacgcg
     3661 ccgccgtgga aatatcgacc tcttgcgctg aagcgtttaa atccgcctgt cggctgcgtt
     3721 cgtaccatac ttgtatctcg taacgtccaa cgggtacacc gtccatcgtc acatgccctg
     3781 tgcgatccga cagtccaaag tagggcgtcg ccaccgcgat caccacagcg ctcatctctg
     3841 ggtggatatt gcagaacaaa tagctcactc cggcccgatc gaatcgcacg gcgcggcttg
     3901 aaccggcttc gtacaaaccg aggtcaaatc gtttgccgtc gaagagagaa aaaatgttgt
     3961 gaaagaaggg gtcccgattt ggaaactgaa cgagggtacc aacctgtaca acgaggatgt
     4021 gcggctggaa agtcttgttg cgctgaacca attgcgggat tggcttcgcg gacgtcccgg
     4081 ccggggttgc acttagaacg ttatcgatcg ggatcagcca aacggcaacg ttcgaggcat
     4141 cggcgatctt aacgctccgc gtgtgctgac tcttgacgcg aggcctaaca acctcaacgt
     4201 ccgcagtgac cggcacttgc tgcgcgaagg catttaccgc gccgacgcac aaccagaaaa
     4261 taacgagcca tccgcatctt cgaggtcccc acttgaacct tcttgggaca gatgctccca
     4321 tcagaataaa tatcctaggc tgagattaat gtggttcgcc gtttgtgacg ctgtgttgtg
     4381 caggagggac gtctgcaacc gtcgatactc cgccgagaaa agcacgtccg agcgtatctg
     4441 ataaatgaaa ttaacgaatg ggctagagtt cctcgaaagc agtatgccgt aagctccctg
     4501 ggttgccgga aagcgctcga gttcgctggc aaaaggactg tcttcgccaa acgcgccatt
     4561 tatctcgaaa ttcgctcgcg gcttgaattt caattgtgcc catccccccg ccgagtcgag
     4621 tccccggaca atggttccct ggtttgccag cgggccagaa agcagcacgt cctggccgat
     4681 accgccgttg aggccaccca tcgcacgccc tcgatagaac tctcctctca gagcgaagag
     4741 tctgccgagg ggtacagtta gatccgcggt gccggcccag ccgtccacat tttgtccgaa
     4801 tccccaatac tgccggccat aatacccacc ggcgccgatc gtcaaagtct ggccggatgc
     4861 cggtacgttc caggccacac gtgccgcata ggcgggttga cctgatttct caccccgggt
     4921 tggagaccgt tcaaacgtcg actgcggaac atctcccgag agcgagtcga gaattccggc
     4981 ctgcacattg agattcgaac tcttcggcaa atcaattcga tgctcgattc gcacctgagg
     5041 aatccagccc cacagattgc cggcataaga gagcggagga acggccagag acgcaagtga
     5101 cgtgggttcg agcggcgcaa aaaataaccc atcctgtccc gcgataaccg acgttgtggc
     5161 ccagtccagg cgaatggtgc cagtgcgcaa gcgtactagc cccatcgaga tgccgttcgg
     5221 cgtgttcgga aatccacctg cgaaatcaaa cttaaggctt gcactagtgt gagctccggc
     5281 aaaatccggt ccgaaggcct cgatgccaac ttgcgactgt ctcagtgacg ctccaaacgt
     5341 ccccggcgaa tcgaacggct gccggggact ggcaatctcc ggaacttcct gatcgtccac
     5401 cgttccccga ttatcaaaaa cgctcaatag cgcgattccg gaaaggcgga ctcgatattt
     5461 cgaggcggct tccaccttcg tctggctttg ctctttcagc tcggcgcgag caagttgctg
     5521 gtcctcctcg agttcggcga cgcgctccgc gatgtcttga ttctgagccg ctgagcccga
     5581 agttgtggaa ctcgtcgatg gttcggaaga atcccctgtt ggcccgcggg atgcataagg
     5641 gccgatctcc cccggattgc tgtgctcaga cgtcgcactg cggttttcgc gctccaactc
     5701 aagtcggaga ctgcgagatt cctctcgatc ccgttgctct tcggcacgca gttcgctcac
     5761 tcgcgagttc aggacctgca cctgatcccg aagtgcgcgg attgaatccg caagctcccg
     5821 cacgtctgcc gctgtccctg tcgcggggga gctcgccggt gcgtcttgtg ctctcaggag
     5881 gacgccgctt agaagtaaga acgctgtaga cacgcccaat ttgaagacta gccttcgaga
     5941 aacgttgctt tgcacggtgg atctccttgc cgacgcgctg ggcccttttt caagttctgc
     6001 aaatctcgtg gcttagttgc ctggttggag tgaatgtcgc cggaaatttt ttcttctacc
     6061 cctcgcctct ttgtagcgta gggctaggtg ccagacgagc caggcctaat cgccaagcgc
     6121 tatcttcggt caagctctga tggcttcctc attttacaaa aatttgacac gtgctcgact
     6181 ttgaaatgac aaaccggcaa ggcaccgccg ctagcgtata ccggccgggt cgatttaagc
     6241 gaaataatcg attttggcca aggggagcca atgagaattg aaaacgtcat caggaaaaac
     6301 gtcaatcggc gatcgttctt gaaacgaggg atcgttgcca gtgcagcaac cgtgaccggc
     6361 ggaatcctgg ccgcccaact ctctgcccgc gaacaagagg acgaaaagag cggaaaactc
     6421 accaagggag atgcggcaat cctcaggttt ctggctgctg cggagatcct cgaaacggat
     6481 ttgtgggtcc aatacaacga acttggagga gttcaagaca acgaggtccc ggggggaacc
     6541 ggaagtatgc cctacaccgt cgctcttcaa gtgctcgact cagacatgcc tcagtatatt
     6601 catgacaaca ccgaggatga attcacacat gaggacttca tcaacgcata tctcgcctca
     6661 aagggggcag acatcgtcga tttagaacga ttccgcacat tgccaggcag caccgcaacc
     6721 gggtctagcg gtaagctgcg gctcacgaat cttatggagc tgaccgttga cacgagctgg
     6781 tggactcgtt atcgcagccg cacaaagaat cccgatttcg gcgacacctt tccgcaagct
     6841 attccgagtt tagccgttgg gatgcatcca gcaattccaa gaacggatgc cgatctgact
     6901 cccggagacc acatccaggc gatcgcgaac actgctggat tccatttcgg taccatcgaa
     6961 cagggcggca caagcttgta cccgtcactg gcccaacgcg taaccagtgt ggaggtttta
     7021 cggatagtcc tcagcatcgg tccgaccgag gccatgcact ttcaaacttg gcaagataaa
     7081 gcgggcaatg cgccccctct gactgatccc agcgatccca cattggtttt tccggacctc
     7141 aattcatctc cgtttggagg cgaggacttt cagacgaatc tcatcatgcc cgagccgacc
     7201 atcttcctta gccgcagctt accggcttgc tcgatcgttc gccccacgga gacaaacgga
     7261 gcggcgaccg cggtcgtcgc atttcttacg gctatgggtc tcttccgcgg gcaatcgccc
     7321 gcgttcaccg cattcctcaa cgatttggcc gaggacgcag acgacgcgcg tcgccaaggc
     7381 caagcggcga gggattagga tcgggcacta acgagcttca ccacgccccc gcgcaatccc
     7441 actggcggaa ggctccaaga atgccggcgc ctccgccagc cggttcttaa agcaacagtc
     7501 caagggactc ttgcattaca gtatctgtac agattagctt tgcttctgga gtgtgtgaag
     7561 aacaggagca agatgcccac ccgagacgac gcttggactt tactctgcga atacactcag
     7621 agcgagagcc tgcgaaagca cgccttggcc gtcgaagctt gcgtgcgcgc ctatgcgcga
     7681 aaggtcggtg ccgacgaaga tctatggggt accacagcgc tgctacacga cttcgattat
     7741 gaacgctggc cgaacgaaga acaccatccc agtgaaggcc atcccggagg tggtgctcgc
     7801 atcctgcgcg accacggcta cccggaagag atggttcgcg cgatcctgtc tcacggtgac
     7861 tacactggtg tccggcgcga aacgccactc gagcacacgc tgtttgcttg cgacgagctt
     7921 gccggatttc tcactgcctg tgcgctggtc aagccgggca agagcatttt ggaggtcgag
     7981 atcgattcgg tgaaacgcaa gctgaaggac aaagcatttg cgcgtggtgt caaccgcgat
     8041 gacgtgcgaa agggcgccga ggaattagct atcccgctcg aagagcacat cgggttctgc
     8101 atcggagcca tgcgtgccgc cgcggatgcg ctcggcctcg acggcaaagc tagcaggacc
     8161 tgacgcccag cggggatgcg gagccaaggg aaaaacgact gatacgttgt cgcgctccgc
     8221 gggatccaat tactgagaag aggtcgcttt ttgacctttt atctgcaact cattcactac
     8281 ctgcgaaaca ttcggaacct ttgatgcgat atttgcgact tctgtgcgcc tggattcgga
     8341 actgacggtg ccgctcaatg tcacaactcc gttcttcact tcgcaactaa cacccttctt
     8401 aaggttgtgt tggacgagag ctgcatccag attcttgcta atgcccttgt cgagatcgga
     8461 attgatggtc ttggcttcac tctccgcagc cttcggggtg acctcaatct ggttcgccac
     8521 aacttggctg ccagccgcgg acttggcgat tgattccgca ttttgcttgt cggcgtcgga
     8581 aggaacgtgt ccgttgagcg tgacgacgcc cttatcccga tcgtcgctaa cagacacgtc
     8641 cttgagaccg gcttggtcta gagacttacg caccgaagca gagacatcgg gcgatttcgg
     8701 tgtattggaa cagcccgaca ttagcagaaa gagcgtaaat aaggctgccg acaatttgaa
     8761 cgctttcacg gtttctccct cctctgttcc acttgtccaa acccgcgtgg accggccaga
     8821 tctaacgact cagtcccaag cctccaagta aatataagac gagcacgatc agcagcactg
     8881 ttccgagaat ccccagtccg ccgcccgcgc cccatcgcgt gtacccgtaa tagccgccac
     8941 ccccgccgaa taagagcacg agcagaacaa ttagcagtaa catgggtttc ctttccgcgc
     9001 acccttacac gccgtaaaca tgagccataa taatttgcct tggcggagag gcacataccc
     9061 tgtaacacgc acggttctgg gaaacgaaat gtaccttcgg gccctgctac tgttaaaaat
     9121 gaaggagata gggggatgat tcttgcaagg ccgtcagatg actgaagcca taggcctcgg
     9181 tacgagggct ggaaggcttg cggccagccg tatccggcga tatgccagga caagaggcgg
     9241 cactcagctg tccaatcaag ggtttatcgg gatctgcgtg aacttgggcg aaggacatca
     9301 tgtcccttat ccacagacga gagtgaaggt tttgacacga agacgggtgc gctagggggc
     9361 cttggcagaa ccagaaggcg actgcacctg taaattattg ttgacccgca ccacgcctgg
     9421 caccgaagca ctgaccgtct ccgatttttt ctttacgtcc gcgctttgaa cctgaccggc
     9481 tagtgtaacg acaccgttcg tggtgcttac ctccacgttg gttaggctcg agccaccaac
     9541 ttctgtcgcc agcttcgcct tgacccgcgt ggtgatttgc gcatcgctaa gctgtcggtt
     9601 gggtggctcg gtcgtattgc agccctccgc gagcatggca agcgaaatca atgtagccgc
     9661 cgctaagaca agcgggcaac gaatgacaag gggcacgcgc ttggtcatga ttctcctctt
     9721 ctcctgggcc gggctttatc tcttgattcc gggtcatggg tttacacgcc accactacat
     9781 ttaacccgtg aacgacgatt ctccagcatc ccgtaggcac tggaatcgag gctgataaaa
     9841 gctgtagcgg caaagtgcct cagggccact ttactgtcat tagtgcccgg taagtcatgc
     9901 cagctcagat ggtcgacgcc ttgaggatca tcgcgagaga atgggcagac tcaggcgatc
     9961 ttacggcaca gcaatgtcgg acgcaggatg aaggtgtacg ttaaaagcgt ggccaagtcg
    10021 ggagtcaatg cgatagatcc gctcggagca gaactccgaa aaaccggaac ttacaacaat
    10081 gttgcaacaa aacggaacgc tctccccaat taggaaatgc aactgaccgc cttacaatag
    10141 gatggatgga gccgag
//
LOCUS       DATNDT010000024         2952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_820560, whole genome shotgun sequence.
ACCESSION   DATNDT010000024 DATNDT010000000
VERSION     DATNDT010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820560"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..865)
                     /locus_tag="VN788_00920"
     CDS             complement(62..865)
                     /locus_tag="VN788_00920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19123.1"
                     /translation="MDADSPNKWSGKWAVVTGASAGIGLALAERLAAGGTHLVLTSRR
                     ADRLEDLGALLATKYGIRVEICPSDLTRLEAPSEIHAFTAGKGIDVELLVNNAGFGAF
                     GYIQDIPTPRLMEMIQVNCAAVVHLTQLYAPAMIARRHGDILIVSSVAAFQALPFNSA
                     YAATKAFDLIFAEGIAEELREFGVHVCALCPGSTTTEFQQVAQQPDRIFRSAETAEKV
                     AQVGLAALAEGRSFVISGAKNRLMVEAERLAPRRFVARMAGKMMRPHTS"
     gene            complement(948..1184)
                     /locus_tag="VN788_00925"
     CDS             complement(948..1184)
                     /locus_tag="VN788_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HXU19124.1"
                     /translation="MVRTIQQAKRYLKIVFGFTLLGIGIIMVVTPGPGTITILIALGV
                     LAAEFVWARRLLDRLKREGTRLRDSVFPPANRAA"
     gene            1430..1912
                     /locus_tag="VN788_00930"
     CDS             1430..1912
                     /locus_tag="VN788_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016873252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HXU19125.1"
                     /translation="MELSAVRMQVPEGANLILGHSHFIKTAEDLYEAVVNTVPGAKFA
                     VAFNEASGPCLVRVEANDDELRQAAVTNARAIGAGHAFVLMVRNAYPINLLSRIRDCF
                     EVCSIFCATANPVEVILAQTELGRGILGVVDGSSPKGVESADDAKWRREFVRKIGYKL
                     "
     gene            2257..>2952
                     /locus_tag="VN788_00935"
     CDS             2257..>2952
                     /locus_tag="VN788_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU19126.1"
                     /translation="MLKLRKVELFGFKSFCEKTPITFSGTGITCIVGPNGCGKSNVVD
                     AISWVLGEQSHKMLRAERMSDCIFNGTARRPPMGISEVTLTLIDPELSEAVAKVMGDD
                     PEPPVAENAGAPVLVEDNQEARLTDFPNPQDAPDQTGDAVIADDLPSSRKGMRKRAAS
                     EKSAVILRPGEVVVGRRLYRSGQSEYLVNGRVARLRDVQEIFMGIGLGPDSYAIIEQG
                     RIGQILNSRPMDRR"
BASE COUNT          647 a          783 c          937 g          585 t
ORIGIN      
        1 acaacgagaa aggcccacga cgaggaacgt ggtcgtcgtg gctaccaaga tacagcgaag
       61 gctacgaagt gtggggacgc atcatcttcc cggccattct tgcgacgaac cggcgtggtg
      121 ccaggcgctc ggcttcgacc atgaggcggt ttttggctcc tgagattacg aagctccttc
      181 cttccgcaag ggccgcgaga cctacctgcg ccaccttttc cgcggtttcg gccgagcgga
      241 agatgcgatc cggctgctga gctacctgct ggaactccgt ggtggtcgag ccagggcaga
      301 gggcgcagac gtggacgcca aattcgcgga gctcttcagc aattccctcg gcgaagatta
      361 ggtcaaacgc tttggtagcc gcgtaggccg aattgaacgg cagtgcctgg aaagcggcta
      421 ccgaggagac aatcaggatg tcgccgtgcc ggcgcgcgat catggcgggg gcgtacagct
      481 gtgtgagatg aaccacggcg gcacagttga cctggatcat ttccatcagg cgcggagtgg
      541 gaatgtcctg gatgtaccca aaggccccaa agccggcatt gttgaccagc agctcgacgt
      601 cgatgccctt gcctgcggta aaagcatgaa tctcagaagg ggcttccagc cgtgtcaggt
      661 cggagggaca gatttcgacg cgaatgccgt acttggtggc tagcagtgcg ccaaggtctt
      721 cgaggcggtc ggcgcggcgg gaggtgagga ccaaatgcgt gccgccggcg gcgaggcgtt
      781 cggccagcgc caagcctatt ccggcactgg cgccagtaac aaccgcccac ttcccggacc
      841 atttgttggg ggaatcagcg tccaccgatg cctcctctgg ataatgagat atgagagcac
      901 aatccgaggc ggcgcaaaag ccgggaaaga tgaagaaact ggaagaatca ggcggcgcgg
      961 ttggcgggag ggaaaacgct atcgcgcagg cgcgtcccct cgcgcttcag gcggtccaaa
     1021 agcctgcggg cccagacgaa ctcggcggca agaacaccga gagctatcaa aatagtaatt
     1081 gtgcccggac ctggcgtgac caccatgatg attcctatgc cgagcaaggt aaagccgaat
     1141 acaattttaa ggtaccgctt ggcctgttgg attgtccgta ccactctatc caccctaaaa
     1201 ccgtgggtcc cgaacccccg cccttcttga agtccaccga gcttcctgta gcttcgatgc
     1261 agccattcgt gagaacgact cgaaaatttc gagcacttca aggaccaacc gcgttacgcg
     1321 cttgctggag aaagacacgg tgctgataat ggacttcggc aagcgcgcca ttccaaaggt
     1381 tttggtcgat tctgaaaaag cgaaaatttt tgcactggag gatgaaaaaa tggagctttc
     1441 cgcagtacga atgcaggtgc ccgaaggcgc aaatctaatt ctcggacact cgcacttcat
     1501 caaaacggcg gaggatttgt acgaagcggt ggtgaacact gtgccggggg caaaattcgc
     1561 cgtggcgttc aatgaagctt ccgggccttg tctggtgcgg gtggaggcga acgacgacga
     1621 actgcggcag gctgcggtga cgaatgcgcg tgcgattggc gcgggccacg cgtttgttct
     1681 gatggttcgc aatgcttatc cgattaactt gctgagccgc atccgcgatt gcttcgaggt
     1741 gtgctcgatc ttctgcgcta cagctaaccc ggtggaggtg atcctggcgc aaacggaact
     1801 gggacgcggc attttgggag tcgtcgacgg gagctcgccg aagggtgtgg agagtgcgga
     1861 cgacgcgaag tggcgccggg aattcgtccg caaaatcgga tacaagctgt agagcattgc
     1921 agctcctgca tagtgtttcg gcctgaatag gcgagaggtt gagaccttgg gacgcctttt
     1981 tgatcgttga cacggaaagc caaagatgct gtgtggtttt tcgtggattt ttatgcgggg
     2041 tgtgtcgttt taggggcttt tcgacaaaag cgcgctttac aggtttccat caggcgtgct
     2101 agtttgtctt cgttaccgcc gaaacgctgg cgccaggcgg cagggccatc aatatcgacc
     2161 ctccctacgt ttttgtctcg atagaccttc ccgaaaacaa tttacagacg tttcttccgc
     2221 cgccgagcgg ggaagagcgt accgagtgag ggacgggtgc taaaactccg caaggtcgag
     2281 ctatttggat tcaagtcgtt ctgcgaaaag acaccgatca cattcagcgg cactgggata
     2341 acctgcatcg ttgggccgaa cgggtgcggc aagtccaatg tggttgacgc gatctcgtgg
     2401 gtgctgggcg agcagagcca caagatgctc cgcgccgagc gcatgtcaga ctgcattttc
     2461 aatggcaccg cgcggcgtcc gcctatgggg atttcggagg tgacgctgac gctgatcgac
     2521 cctgagctat cggaggcggt tgcgaaagtg atgggcgacg acccggaacc gcctgtcgcc
     2581 gagaacgcag gtgctcctgt tctcgttgaa gacaatcaag aggctcggct taccgacttt
     2641 ccaaatccgc aggatgcgcc agaccaaacg ggagacgcag ttatcgcaga cgacttaccg
     2701 agcagccgta aagggatgcg aaagcgggca gctagcgaaa agtcagcagt catcctgcgg
     2761 cccggcgagg tggtggtggg aaggcggctg tatcgctccg gccagagcga ataccttgtc
     2821 aatggccggg tggcgaggct tcgcgacgtc caagagattt tcatgggcat cggcttgggg
     2881 cccgacagtt acgcgattat cgaacagggc cgaatcggcc aaatcctcaa ttcgaggccg
     2941 atggaccggc gg
//
LOCUS       DATNDT010000025         5081 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1950833, whole genome shotgun sequence.
ACCESSION   DATNDT010000025 DATNDT010000000
VERSION     DATNDT010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5081
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1950833"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..460
                     /locus_tag="VN788_00940"
     CDS             185..460
                     /locus_tag="VN788_00940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19127.1"
                     /translation="MSITVYFRHKTNHGWRYEALGIGRRPEAAKNGPFYIRVREEGES
                     RWEKHLSELLAKRAAESAPVERKAEALGLLPDDLTNETNSNRTPIHG"
     gene            complement(913..1740)
                     /gene="fdhD"
                     /locus_tag="VN788_00945"
     CDS             complement(913..1740)
                     /gene="fdhD"
                     /locus_tag="VN788_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HXU19128.1"
                     /translation="MSRVKPAIFVTQVTAWENGAARRVDDCLAAEEPLEISVNGQPFG
                     LTLRTPGNDLDLVAGLLFSEGVISGYGQIASIEMGADEGRRTSGNHVSVELTSGMAVA
                     STATGRKFTAGSACGICGKAAIDDLKHRGLSRPDRGSRFEADILCTLPEKLRQAQTNF
                     SRTGGLHAAALFDARGNLLVLHEDIGRHNAVDKVIGSALKSKKIPLTGHILLASGRGG
                     FEIVQKAIVAGVPLLASVSAPSSLAVQLAREMGITLVGFLRGRRFVIYANDERLSRR"
     gene            1905..4955
                     /gene="fdhF"
                     /locus_tag="VN788_00950"
     CDS             1905..4955
                     /gene="fdhF"
                     /locus_tag="VN788_00950"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HXU19129.1"
                     /translation="MRHLEQFPGTEVHKTDSGGFEPPGEKSRARFEITIDGSPCQTHE
                     GERLIDLANRLGPEIPQVCYHPQLGPIQTCDTCLVEVNGKLSRACATVIEPNMRVSTN
                     TPAVKRAQLQAFDQILANHNLYCTVCDNNNGNCTVHNTTALLDVQHQGIPFQQKPYEI
                     DSSNPFYRYDPSQCILCGRCVEACQNVQVNETLSIRWEDEHPRVLWDGGKPVGESSCV
                     SCGHCVSVCPCNALMEKSMLGRAGFFTHLPTKTLNQMIDFVKGVEPETGFGPILGFSA
                     AEAAMRQERVRRTKTVCTYCGVGCTFDVWTKDREILKIEPFAGPANGISTCIKGKFGW
                     DYTNHGDRLRQPLIRDGASFREASWEEALSLIGETLGKIKRRHGPDSIGVIASSKNTN
                     EESYLAQKLARAVIGTNNIDNCSRYCQSPATQGLFRTVGYGGDSGSIADIAKATLVVI
                     IGSNTAESHPVLATRIKRAHKLHGQKLIVSDIRKHEMARRADIFLRPRPSTDLVWLCA
                     AARYIVENDLHKRQFIAEWVNGFEEYRRSLEPFTLEFAEKNTGLSAQTIADVARMIAT
                     EERVCILWAMGVTQHCNGSDTSTAMSNLLLLTGNYKRPGTGAYPLRGHNNVQGASDFG
                     SMPQYYPGYQKVEDDSARGNYEKGWDVKLPSGKGLDNHQMVDAIFDGKLRAMYIIGED
                     MITADSNSHRVGEGFSKLDFLVLQDCFFSETCRYADVVLPASPALEKEGTFVSTERRI
                     QRLYEVFPPLGESRPDWQIIQDVANHLGANWNYQHPSEIMHECAMLTPLFAGVSYERL
                     EGYKSLQWPVAADGTDQPLLYTQKFPMPDGKAKFHPLEWTPPCEESDAEYSLHLNNGR
                     LLEHFHEGNMTYRVAGIREEVPRQFLEVSPDLAQELQIADGRWVELASRYGIVRVPVL
                     ITDRVQGKELYLPLNSSVSPVNVLSSSHTDPYVHTPAYKEIAVKLRVLPDKGPSPLPR
                     SNHRYGHPTPQRGVEVERKWKRTDYRTPGLQLVQIQSDGKNGNG"
BASE COUNT         1219 a         1495 c         1318 g         1049 t
ORIGIN      
        1 agacacaaga tcaaacaagg ctttgcaagg tcgtacaagg ctgtataatc aacgattcga
       61 atcccaccct ctccgccata tccaaatacc cttataccct tacaaaagga ctattctccc
      121 tccatgctac attttccggg cgacttgcag gcccttgcac ggtcttgcag ggaggctcaa
      181 aacgatgtcc atcaccgttt atttccgaca caaaacgaat cacggttggc gatacgaagc
      241 cctcggaatc ggccgcaggc ccgaagcggc taagaacggc cctttttaca ttcgcgtccg
      301 cgaagagggc gaatcccgct gggaaaaaca cctctctgaa ctcctcgcaa aaagagcagc
      361 cgaatccgcg cccgtcgagc gcaaggccga ggcactcggt ttactgcccg acgatctgac
      421 gaacgaaacg aactccaacc gaacgcccat tcacggttga acactatagc gagcagtggc
      481 agttcggcga ccgcgtagca aggccgtcta caagaatgta tgctcctccg ccatcgctca
      541 aaagcgggag caaaactgtt ttcggatttt gcccatgtgt gttcatcggg ccaaacttcc
      601 ttcgcggcaa tgagaaccgg agttagcttc cgacccattc aagggaaacg tcattagtgt
      661 cgcggttcct cgcgatgcaa tcgcttgctg ggccgaatct ggccaggttg ccatcaaagg
      721 catgcatctt ccagcaggcg gagagcagtt gaaaatcctc attgaaaagg actttcgttg
      781 catcgagacg cgtacggatg aggaggaatg ggaccgcttt tcgaacccct ccgtttgcaa
      841 acccgcggag gatatcactc catcctggtg atactacgag agccaccgca accaagcatc
      901 aggtattact gcctaacgcc tgctcaggcg ctcgtcattg gcatagatga cgaatctgcg
      961 ccctctaaga aatccgacta acgtgattcc catctcgcgc gccagctgca ccgcgagact
     1021 cgatggtgcg gaaactgaag cgagcagcgg cacacctgcg acgatcgctt tctgcacaat
     1081 ctcaaacccg ccccgtccgc tggctagaag aatatgcccc gtcagaggaa tcttcttaga
     1141 cttcagcgcg gagccaatca ctttatcaac cgcattgtgg cgaccaatat cctcgtgaag
     1201 taccaagagg ttcccgcgtg catcaaacag cgccgccgca tgcaggcccc ctgtgcggct
     1261 aaaatttgtt tgagcttgcc gaagcttttc cggcagagtg cacagaatgt ctgcctcaaa
     1321 gcgcgatccc cgatctggcc gtgagagccc acggtgcttc agatcgtcaa tcgccgcttt
     1381 gccacagatt ccgcaggccg atccagccgt gaatttccgc ccggtggcag tgctggcaac
     1441 cgccattccg ctggtaagct ctacggaaac atgatttccg ctcgttcgcc ggccttcgtc
     1501 cgcgcccatt tcgatggacg caatctgtcc gtaacccgaa ataactcctt ccgaaaacaa
     1561 aaggcccgcg acaaggtcaa gatcgttgcc tggagtacgc aacgtcaagc caaatggttg
     1621 accgttcacg ctgatttcca gcggctcttc ggcggcaagg caatcgtcaa cacgacgtgc
     1681 ggcgccattc tcccatgcgg ttacttgcgt cacgaaaatc gcgggcttta ctcgggacat
     1741 ttttattgca cggcgtttgc gtgccctgtg cgaaattata cgcccgatgg tgcaatgccc
     1801 ttcacgcgca aaccactcag taaaatacag cgcagcttgt attgagagat tgaagtgcga
     1861 atagatagaa gtagaggtat aaccttccaa ggcggccaac ttcgatgcga cacctcgagc
     1921 aattcccagg aacggaagtc cataaaaccg acagcggcgg tttcgaacct cccggcgaga
     1981 agagccgtgc ccgtttcgag attactatcg acggctcacc ctgccagacg cacgaaggag
     2041 aacggctaat cgatctcgcc aaccgacttg gccccgaaat ccctcaagtc tgctatcacc
     2101 cgcaactcgg cccaattcag acttgcgata cctgccttgt cgaagtgaac ggaaagcttt
     2161 cgcgcgcatg cgcgacggtt attgagccca acatgagggt ctctacgaat actcccgctg
     2221 tgaaaagggc tcaattacag gctttcgatc agattctcgc gaatcacaat ctctattgca
     2281 ccgtctgtga caataacaat ggcaactgta cagtccacaa cacgacggcc cttctcgacg
     2341 tgcagcatca gggcattccg ttccagcaga aaccgtatga gatcgattcc tctaatcctt
     2401 tttaccgata cgatcctagc caatgcatcc tctgcggacg gtgcgttgaa gcctgccaga
     2461 acgtccaggt aaatgagact ctttccatcc gctgggaaga cgaacatcca cgcgtgcttt
     2521 gggacggcgg caaacccgtt ggcgaatcga gctgcgtctc ctgcggacac tgcgtgtcgg
     2581 tgtgtccgtg caatgcttta atggaaaaat ccatgctcgg gcgtgctggt tttttcacgc
     2641 atttgccgac caaaacgctg aatcagatga tcgatttcgt caaaggcgtc gaaccagaaa
     2701 cgggtttcgg cccgattcta ggattttccg ccgcggaagc ggcgatgcgg caagagcgcg
     2761 tccgccgcac gaagaccgtc tgcacatact gcggcgtcgg ctgcactttc gacgtgtgga
     2821 caaaagatcg tgagatcctg aaaatcgagc ctttcgcggg tcccgcgaat ggcatttcga
     2881 catgtatcaa aggcaaattc ggatgggact atacgaacca tggcgatcgt ttgcggcagc
     2941 ctttaattcg cgacggggcg agtttccggg aggccagctg ggaggaggcg ctcagcctca
     3001 ttggtgaaac gctcggcaaa atcaagcgcc gacatggccc cgattccatc ggcgtgatcg
     3061 catcctcaaa gaacacaaac gaagagagct atcttgcgca gaagcttgcc cgcgcggtga
     3121 ttggcactaa caacattgat aattgctcac ggtattgcca gtcgccggcc acgcaagggc
     3181 ttttccggac tgtcggctac ggcggcgact ccgggtccat tgcagacatc gccaaggcca
     3241 cgcttgtcgt cataatcggc agcaatacgg cggagagtca tcccgtcctg gccacacgca
     3301 tcaagcgcgc ccacaaattg cacggccaaa agctgatagt ctcggacatt cgtaagcacg
     3361 agatggcacg gcgtgccgac attttcctgc ggccccgacc cagcacggac ctcgtatggc
     3421 tctgcgccgc cgctcgatac atagtcgaga acgatctgca taagaggcag ttcatcgccg
     3481 agtgggtcaa cggcttcgaa gaataccgca ggagcctcga acccttcacg ctcgaatttg
     3541 ccgaaaagaa caccggtctt tccgcgcaaa ctattgcaga tgtggcccgt atgattgcca
     3601 ccgaagagcg cgtttgcatc ctgtgggcga tgggcgtaac gcaacactgc aatggatccg
     3661 atacttccac agccatgtcc aatcttcttc tgcttaccgg aaactacaag cgaccgggca
     3721 ctggggctta tccactgcgc ggccacaata acgtgcaggg cgccagcgat ttcggatcga
     3781 tgccccaata ttatcccggc taccagaaag tggaagacga tagcgcgcgc gggaattacg
     3841 aaaaggggtg ggatgtgaag ttgcccagtg gaaaagggct ggacaatcac cagatggtcg
     3901 atgccatctt cgatggcaag cttcgcgcca tgtatatcat cggcgaagac atgatcacgg
     3961 cagattccaa ctcgcatcgt gtaggcgagg gcttttcaaa actggatttc ctcgtcttgc
     4021 aggattgttt ctttagcgaa acgtgccgct acgcggatgt ggtccttccc gcgagccctg
     4081 cgctggagaa ggaaggcacg ttcgtcagca ctgaacgccg cattcagcgc ctttacgaag
     4141 tctttccgcc gctcggcgaa agcagaccgg attggcaaat catccaggac gtcgctaatc
     4201 acctcggagc caactggaat taccagcacc cctcggaaat catgcacgaa tgtgcgatgc
     4261 tgaccccact atttgccggc gtctcctatg aacggctcga gggatacaag tctctgcaat
     4321 ggccggtagc ggcagacgga acagatcaac cgcttcttta cactcagaaa tttcccatgc
     4381 cggatggaaa agcaaaattc catcctctcg aatggacccc gccttgcgag gagtcagatg
     4441 ccgagtatag cctccacttg aacaacggcc gcctgctcga gcactttcat gaagggaaca
     4501 tgacgtatcg tgtcgctggt atccgcgaag aggtcccgcg ccaatttctc gaagtctcgc
     4561 ccgacctcgc gcaggaactt caaatcgccg acggccgatg ggtcgaactt gcgtcgcgct
     4621 atggaatcgt tcgcgtgccg gtcttaatca ccgaccgtgt tcagggcaaa gagctttacc
     4681 tgccgctgaa ttccagcgtg agtcccgtta atgtgctcag tagcagtcac actgacccat
     4741 acgttcatac gccggcttat aaagagatcg ccgtgaagct tcgcgttctc cctgataaag
     4801 gcccaagccc tttgccacgc tccaatcatc gctatggcca tcccacgccg cagcgaggcg
     4861 tcgaggtcga acgcaaatgg aaacgcacgg actatcgcac gcctgggttg cagctcgtac
     4921 aaatccaatc cgatgggaaa aacggaaatg gctaagcctg tgcccacaat tgtcaaggga
     4981 tcgcaaatat ctagctccaa cggcgttcgg gaagggctgc agcgtgagct agcgaaagct
     5041 ccagttgagc atgccgaagc tctcctgtcc gcttatgaac t
//
LOCUS       DATNDT010000026         4592 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3975705, whole genome shotgun sequence.
ACCESSION   DATNDT010000026 DATNDT010000000
VERSION     DATNDT010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4592)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4592)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4592
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3975705"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /locus_tag="VN788_00955"
     CDS             complement(<1..1253)
                     /locus_tag="VN788_00955"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HXU19130.1"
                     /translation="MMNRTIAMISLAACMGWAASSGLTPEGQRWWKHVEYLASDQLEG
                     RGTGTAEYQKAAEYVASRFHDAGLKQAGTQDYFQPVRFDVTRLDETRSAMALLDGGTR
                     IPIAIGPDAILGKRGETGSFEAEAVFAGYGLTIPEANYDDLRGLDLNGKIVVYLSGAP
                     APISGPLKSHYQSFEQRWRFLKAAGAVGFAAILNPKAMDVPWSRVASSRGNPAMTLID
                     AGNTDDKGLRFSLTINPTAANRFLAGSGHTVAELLADADSRRLLPHFPLQRRIAVTQT
                     FVHSNAESPNVAGVLEGDDLNLRNEYVILSAHLDHLGIGAPVHGDRIYNGALDNAAGI
                     ASLIEIARHLHDSGVRLRRSVLFLAVTAEEKGELGSQYFAAHPTVPSGGIVADINMDM
                     FMPLFPLRYLEVQGLGESTLGASIR"
     gene            complement(1294..1770)
                     /locus_tag="VN788_00960"
     CDS             complement(1294..1770)
                     /locus_tag="VN788_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU19131.1"
                     /translation="MSEISDLLERFRGGPELVADTIAGATDAELDFAPAPGKWTMRQI
                     VAHLADTELVMAARFRRMIAEDRPRFEEFDQDAWAQSLDYSKRLPAQSIETLTLIRGD
                     NYALLKDISEAAFERTGVHPRRGEMSVLQLVKLSADHVEKHSGHLRERREQWRRRS"
     gene            complement(1777..2424)
                     /locus_tag="VN788_00965"
     CDS             complement(1777..2424)
                     /locus_tag="VN788_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXU19132.1"
                     /translation="MARKRVVGFSLGGAILTAACLSYYYRRAGRDEQNRFLQLLDATP
                     GEQIGEVGAGHGDFMLLAAARVGPNGRVYANELDPKRAGELRTKAAKQKLAEIHVIEG
                     AEDSTRFPDNCCDTIYLRGVYHHFTKPAAVDASILRALRPGGRLIVVDFPPRLLLKPW
                     TPKGIPANRGGHGIPQKILIDEITDAGFRLDHVEAHWPNGFYCVVFRKRAAAMVA"
     gene            2821..3294
                     /locus_tag="VN788_00970"
     CDS             2821..3294
                     /locus_tag="VN788_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXU19133.1"
                     /translation="MEERIDYKKAKPEAIQPLFDIERYLHQSGLEASLQELVKIRASQ
                     LNGCAFCIDMHTKDARFRGESEQRIYELNAWRETPFFNDRERAALAWTEAVTLVSETH
                     VPDDVYEEARKQFSDQELMDLTLAVAAINMWNRLAIAFRTVPGSYQVQAAKVARS"
     gene            complement(3597..4277)
                     /locus_tag="VN788_00975"
     CDS             complement(3597..4277)
                     /locus_tag="VN788_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011037901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-dependent dioxygenase"
                     /protein_id="HXU19134.1"
                     /translation="MLLPIPDVLTAEQVRRARQLLDQAEWVDGRVTAGHQSARAKDNM
                     QLPEGSPAARELGAMILSALEQNALFISAALPARVFPPLFNRYQGGQSFGTHVDNAIR
                     QVTGTPHRIRTDLSATLFFTGPDEYDGGELVVEDTYGTHNVKLPAGHLILYPATSLHH
                     VRPVTRGARIASFFWIQSMVRDDGERTLLFDLDVAIQRLNGEGSGQPSSVQLTGVYHN
                     LLRRWADI"
     gene            complement(4299..>4592)
                     /locus_tag="VN788_00980"
     CDS             complement(4299..>4592)
                     /locus_tag="VN788_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU19135.1"
                     /translation="WSTYRLPWRWETGFGGNYVSSRTASSTAPFDPVTGLVKEVPGYW
                     VFNAMASHRLTEHIDLQVNVNNIANRYYYDELHPAHIVLGPGRSALIGLKFKF"
BASE COUNT         1023 a         1337 c         1300 g          932 t
ORIGIN      
        1 tcccggatgc tggcgccgag agtcgattcg ccgagtccct gcacttcgag ataacgcagc
       61 gggaacagcg gcataaacat atccatgttg atatccgcga caataccgcc ggacggcacg
      121 gtgggatgcg cggcgaaata ctgggagccg agttcgccct tctcctccgc ggtcaccgcg
      181 agaaataaaa cagaacggcg aagccgaacg ccggaatcgt gcagatggcg cgcaatctcg
      241 atcagcgaag cgatacccgc tgcattatcc aaagccccgt tgtaaatgcg atcgccatga
      301 acgggagcgc cgatgccaag atgatccagg tgcgcgctga ggatcacata ctcgttccgc
      361 aaattgaggt cgtctccttc gagaacaccc gcgacattcg gtgattcagc gttagagtgg
      421 acgaaagtct gagtcacggc aatacggcgc tgcagaggaa agtgcggaag tagccgtctg
      481 ctatcggcat cggcaagtag ctccgccacg gtatggccgc tacccgcaag aaatcgattg
      541 gccgctgtcg gattgatggt cagcgaaaac cgcagaccct tgtcgtccgt gttgcccgcg
      601 tcaatcagcg tcatcgccgg attcccccgc gatgacgcca cgcgactcca tggaacatcc
      661 atcgccttcg gattcagaat tgccgcgaat ccgacggccc cagcggcttt caaaaatctc
      721 cagcgctgct cgaacgattg gtaatgcgat ttgagcggtc cagagatcgg cgccggcgcg
      781 cccgagagat acactacaat cttcccgttg agatcgaggc cgcgcaaatc gtcgtaattc
      841 gcttccggaa tcgtgagtcc gtaaccggcg aacaccgctt cggcttcaaa cgaacctgtt
      901 tctccacgct ttccgaggat tgcatccggg ccgatcgcaa tcgggatgcg cgtgccgcca
      961 tcgagcagcg ccatggcgga gcgggtctcg tcaagcctcg tgacgtcgaa cctgaccggc
     1021 tgaaagtagt cttgcgttcc cgcctgtttc agtccggcat cgtgaaaccg cgaagcgacg
     1081 tactcagccg ctttctgata ttccgccgtg ccggttccgc ggccttcgag ctggtcggag
     1141 gcaagatact cgacatgttt ccaccaacgc tgcccttccg gcgtgagccc gctcgatgcc
     1201 gcccatccca tgcatgcggc gagtgagatc atcgcgatcg tgcgattcat catggttttg
     1261 aacgcaccag ctacggcctg ttatccacgc ttgttaagac cgccggcgcc attgctcacg
     1321 ccgttcacgc aaatgaccgg agtgtttttc gacatgatcg gctgaaagct tgaccaattg
     1381 cagaacactc atctcgccgc gtcgcggatg aacgcccgtg cgctcgaatg ccgcttcaga
     1441 aatgtctttc agcaatgcgt aattgtcgcc gcggatcagt gttagcgttt caatcgactg
     1501 tgcaggcaag cgtttcgaat aatcaagact ctgtgcccag gcatcctggt cgaactcctc
     1561 gaaacgaggc cgatcctcag cgatcatacg gcggaatcgc gcggccatca ccagttccgt
     1621 atcggcaagg tgtgcgacga tctgccgcat ggtccacttg cccggcgccg gtgcgaaatc
     1681 caactccgca tcggtggctc ccgcaatggt atccgccacc aattccggac caccgcgaaa
     1741 acgctccaac agatcggaaa tttcgctcat atacgcctac gctaccattg ccgccgcccg
     1801 cttccgaaac acgacgcagt agaatccatt gggccaatgt gcctcaacat gatcgagccg
     1861 gaaacccgcg tccgtgattt catcaattag aattttttgc ggaatcccat gtccgccgcg
     1921 attcgccggg atgcccttgg gtgtccaggg tttcaggagg agacgcggcg gaaaatcaac
     1981 aacgatgagc cgtccgccag gacgcagcgc ccgcagaata ctcgcgtcca cggctgccgg
     2041 tttcgtaaag tgatggtaga cgccgcgcag gtagatcgta tcgcagcaat tatcggggaa
     2101 acgggtcgaa tcttccgctc cctcgataac gtggatctca gcgagctttt gtttcgcggc
     2161 cttcgtcctc aattcgccgg cccgcttcgg gtccagttca ttcgcgtaga cacgcccgtt
     2221 cggcccgacg cgggcggcgg ccagaagcat gaaatcgcca tgaccagcgc ccacttcgcc
     2281 gatctgctca cccggcgtcg cgtcgagtaa ctgaagaaac cgattttgtt cgtcgcgccc
     2341 ggcccgtctg tagtagtaac taagacaggc cgctgtgaga atagcgccgc cgagactgaa
     2401 acccacgacc cgttttcttg ccatctcttt caggaagacg gcgatagtat gccggaagtt
     2461 tgaaactgcg tttcggtagt gcgctaaatg tgtgtggcga tgaactcaag cgcatgctcc
     2521 cgaataacca aaatggcaat cgtcgagcaa actatagcca tgttaatcgt agacattcgt
     2581 agctagcact tgaatgtggc gcctcttcgt aaaaattggc aaccgatgtg ggcgggcaat
     2641 cttgccctac atcgggcaac cggaattgtc agaattagct gggatacgac gctagcgcgg
     2701 cagatcactg cgatttttga tggagtcttc ggaaagcgac gatcgcgttt gtcgaattta
     2761 gcgcactacc cgtttgtcac gccacaattt caggggcaca atgggaaata ggacaacaat
     2821 atggaagagc gaatcgatta taaaaaagcg aaacccgaag cgatccagcc gcttttcgat
     2881 atcgaaagat atcttcacca gagcgggctg gaagcctcgt tgcaggaact ggtcaaaata
     2941 cgcgcgtcgc aattgaacgg ctgcgcattt tgcatcgaca tgcacacgaa ggatgcacgc
     3001 tttcgcggtg aatccgagca gcgcatttat gaattgaacg catggcgcga gaccccgttc
     3061 ttcaacgatc gcgagcgggc ggcgctggcc tggacggaag ccgtaacgct cgtttccgag
     3121 acgcacgtgc ccgacgacgt gtacgaagaa gcaagaaagc agttcagcga ccaggagctg
     3181 atggatctga cgcttgccgt cgcggcgatc aatatgtgga accggctcgc gatcgcattc
     3241 cggaccgtgc cggggagtta ccaggtgcaa gcggcgaaag tggcccgatc gtgacggacc
     3301 cgcgctggcc ggtggcgtaa gctttctccg actttgccgg accagcgtct agccgcgtat
     3361 tttgcgctca cattatgatc tgctggccgc tcgcgccgat aaccgcgctc ctaaaactgg
     3421 ccggctgata tttcccggca cggttgtgag cgaagacatt cgcgaggatc gcctcgaccg
     3481 ggagccatgc ggagctacga ggccgggacg gagccgaaat cacgctcatc gctccggcca
     3541 cggccctgac cagaaccact gttccggcga ggcaaaccta tagcagctta tgagactcat
     3601 atatctgccc atcgtcgaag cagattgtgg tagacaccag ttaactgcac ggaagatggt
     3661 tggccagatc cttctccatt gagccgctga atcgcaacat cgaggtcgaa cagaagcgta
     3721 cgttcgccgt catcgcggac catactttga atccagaaga aggaagcaat gcgtgctcca
     3781 cgcgtcaccg gtcgcacgtg atgaaggctg gtggcgggat acaggatcag gtgaccggcg
     3841 ggcagcttta cgttgtgcgt tccgtacgta tcttcgacga ccagttcgcc accatcgtat
     3901 tcgtcgggcc cggtgaagaa gagcgtcgct gagaggtcgg tacggatacg atgtggcgtg
     3961 ccggtcactt gccggatggc attgtccacg tgcgtaccga acgattgacc gccctgatag
     4021 cgattgaaca gcggcgggaa tactcgcgcg ggaagggccg ccgagataaa gagagcgttt
     4081 tgttcgagcg cggaaagaat catggctcct agctcgcgag cggcgggcga tccttccggc
     4141 aattgcatat tgtctttggc acgggcggat tggtggccgg ccgttacgcg gccatccacc
     4201 cactcggctt gatcgagaag ctgccgggct cgacgaactt gttcggcagt caatacatca
     4261 ggaataggca gtaacatcgg gctgagaaat ctcctcggtc aaaacttgaa cttcaatccg
     4321 atgagtgctg agcgtcccgg accaagcacg atatgggcgg gatgcaattc atcgtagtaa
     4381 tatcgatttg cgatattgtt gacattcacc tgcagatcga tatgctcggt cagccggtga
     4441 gacgccatcg cattgaagac ccagtatcca gggacttcct tgacgagccc cgttaccgga
     4501 tcaaacggcg ctgtcgaact tgccgtgcgg ctggagacat aattcccgcc gaatcccgtc
     4561 tcccatcgcc acggcaaacg atacgtgctc ca
//
LOCUS       DATNDT010000027         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3441675, whole genome shotgun sequence.
ACCESSION   DATNDT010000027 DATNDT010000000
VERSION     DATNDT010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3441675"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1005
                     /locus_tag="VN788_00985"
     CDS             <1..1005
                     /locus_tag="VN788_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU19136.1"
                     /translation="TRVVEATAEVLFGDPRAVIKVDCAEFQHSHEIAKLIGSPPGYLG
                     HRETHPLITQEALAQYHTDKLKISFLLFDEIEKASDALWQLLLGILDKATLTLGDNRR
                     VDLSQTMIFMTSNLGGGEITELMTGGLGFAPEVRAESKPRLDEKVERTASEAAKRKFA
                     PEFMNRIDKVVVFHPLRSQQLEQILEIELGMVQQRVLETAKGRFLFRLTQPAREFLLR
                     EGTDLKYGARHLKRAIERHLVYPLASLLATNQVTLGDVLSIDWSAGASVLTFLKEAEG
                     ALVPVISPEPAPLAGAAAAISDGRELSFPTPVTAAAEVPLPLPNHRSAPTEKAKNEKG
                     "
     gene            complement(1309..2286)
                     /locus_tag="VN788_00990"
     CDS             complement(1309..2286)
                     /locus_tag="VN788_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19137.1"
                     /translation="MRKRTYVTFLGIALFLVGIAPRADAGSLSTSVIGMFPKEIGEFA
                     YADLKSARKFPWFPQLRDQLLPSRFRQFEKFLSSAGVDPDTQVDELAWGGVAAARGKG
                     EQVVGVALGTFDPSSTEARFKQRKLPIIEVHGYHLYAFGTGSDPDDILFMFIDPNTAA
                     FGHRSALEKLIDVRTGMAESLLTNTTLFPLINEANGTGIIWAALNKDYSNLAMQQLIP
                     QANQFPQAAAIIKRIRAMTISVKVENGVDTHFQAVCSSPDDANLLSAALQAGLMYRRY
                     QESQSNPDLAGALDQVQVGPAGDRLKVDVPVTQDQLLALIRSHAFATPM"
     gene            complement(2321..3856)
                     /gene="ftcD"
                     /locus_tag="VN788_00995"
     CDS             complement(2321..3856)
                     /gene="ftcD"
                     /locus_tag="VN788_00995"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HXU19138.1"
                     /translation="MKRLVECVPNFAEGRDLSKVDAIAAAIREVPEVFLLARESDADH
                     NRSVITLAGDPEGIVEAAVRAVGKASELIDLTRQTGAHPRIGATDVVPFIPLEGVSLE
                     DCVILAKKAGREIWERYRIPVFFYEAAAQRPDRVNLENIRRGQFEGLREEILRNPERA
                     PDVGDARLHPTAGGTVVGARKFLIAYNINLNTPDVAIAKKIAKTIRFSSGGLPFVKAM
                     GVDLRARNLAQVSMNLTDFEQTPIHLVFETVKREAERLGVGIVGSEIVGLIPRRAIEM
                     TAENYLKVENFSPAQVLENRLEESLMESGANASLAAIVTPFLEALAQPTATPGGGSAS
                     ALAGALAASLGQMVAGLSSIKKSGAAHKERLDEAVKEFDAASRRLAAAIDRDAESFQS
                     VMAAYRLPNGTEDDREQRHTAIQNALLDAIEAPLEIAREAAQLFERLGQLESLSSPSM
                     LSDVRVARLMASASVRGALENVAINLQSVTDARVDERVRSECRSLTLRITESPSEAGR
                     KDI"
     gene            complement(3860..>4229)
                     /locus_tag="VN788_01000"
     CDS             complement(3860..>4229)
                     /locus_tag="VN788_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU19139.1"
                     /translation="GCGFHLGTGCYAPARKLIDAGAIVALATDFNPGTSPTLSMPMIL
                     SLACSQMRVIPAEAISAATINAAYSLREQDRIGSLEEGKYADLAAFNVASYREIPYYF
                     GMNLCNLTMKRGVVIHPNKN"
BASE COUNT          908 a         1214 c         1167 g          940 t
ORIGIN      
        1 acccgcgttg tcgaagccac agccgaagtg ctgttcgggg acccccgcgc cgtgatcaag
       61 gttgactgcg ccgaatttca gcattcgcac gaaattgcca agctcattgg ttctcctccg
      121 ggatatttgg ggcaccgcga aacccatccg cttatcactc aggaggccct cgcccagtat
      181 cataccgaca agctcaagat cagctttttg ctgttcgacg aaatcgaaaa ggccagcgac
      241 gctttgtggc aattgctact gggcattctc gacaaggcaa cgctgactct gggcgacaat
      301 cgccgcgtcg atttgtcgca gaccatgatc ttcatgacct cgaatctggg aggcggagaa
      361 ataacggaac taatgaccgg cggcctcggt tttgcgccgg aagtccgagc cgaatcgaag
      421 cctaggcttg atgaaaaggt cgaacgaacc gcctcggagg ctgcgaagag aaaattcgct
      481 cccgaattta tgaaccgcat cgataaggtc gtcgttttcc atcccttgcg gtcgcagcag
      541 ctcgagcaaa ttctcgagat cgagttggga atggttcaac agcgcgtcct cgagaccgcg
      601 aaaggacgct ttctcttccg ccttactcaa ccggccaggg aattccttct gcgcgaaggg
      661 acagatctga aatatggagc gcgccacttg aagcgcgcta tcgagcgcca tctggtttat
      721 ccgctggcca gcttgctcgc aaccaaccag gttactctgg gtgacgtcct ctcaattgac
      781 tggagcgcag gcgcctcggt gttaacgttc ttgaaggaag ccgagggggc tctcgttccc
      841 gtgatttcgc ccgagccggc acctctcgcc ggcgccgctg ctgcaatctc cgatggaagg
      901 gagttatctt tcccgacccc ggtgacggcc gctgccgagg ttccgctgcc tctccccaac
      961 catcgcagtg cgccgacaga aaaggccaaa aacgagaaag gataggcgct ctgctggtgg
     1021 ttaccctctg ttctcaatgc gacaagtctg agagcaaatg ctcctgcgat aagttttgct
     1081 gtttttgcca gtcgcaagag cacgtccacc tctgcgtgga cggactgtac tattgtcccg
     1141 attgccgcga agcgtgcgat gtgcgtttag ctgacacgga tgatcatcac taaggtcacc
     1201 tagcggaaaa cgatcttcct ccccgtaaaa ctacctctta gatggatgtg gagctttttc
     1261 gaggacaggc tatttcagag gcgcctgagc aggggagagc tcaactcgtt acatcggtgt
     1321 ggcgaatgcg tggctgcgaa ttaaagcgag cagttgatcc tgcgttactg gcacatcgac
     1381 cttcaggcgg tcgcctgcgg gacctacctg cacttgatca agagcgcctg ctagatctgg
     1441 atttgactgc gattcctgat agcgccggta catcaggccg gcctgcagtg cagccgaaag
     1501 tagattagcg tcgtccggcg aactgcaaac agcctgaaaa tgcgtatcca cgccgttttc
     1561 aaccttcacg ctgatcgtca ttgcgcgtat gcgcttgata atggcggccg cctgcggaaa
     1621 ctggttggct tgcgggatca gttgctgcat ggccaggttt gagtagtctt tattcagcgc
     1681 cgcccagatg atacccgtcc cgttggcttc attgattagt gggaagagcg ttgtgtttgt
     1741 cagtagactc tcggccattc ccgtgcgcac gtcgatgagc ttttcgagcg ccgagcggtg
     1801 tccgaaagcc gccgtattcg gatcgatgaa catgaataga atgtcatcgg gatctgagcc
     1861 tgtgccgaac gcgtagagat ggtagccgtg aacctcgatt atgggtagct tccgctgttt
     1921 gaatcgcgct tcggttgacg aaggatcgaa agttcctaac gccactccca ccacttgttc
     1981 tcccttgcct cgcgctgcgg ctacgccgcc ccaggcaagt tcgtccactt gcgtatcggg
     2041 atcgacgccg gccgaagata gaaacttctc gaattggcgg aagcggctcg gaagcagctg
     2101 gtcgcgaagc tgtggaaacc acggaaactt ccgtgcggac tttagatcgg cataggcgaa
     2161 ctcaccgatc tctttcggaa acatgcctat cacggaagtg ctcagggaac ccgcgtcagc
     2221 tctcggagca atgcccacca gaaatagagc tatcccaaga aaagttacgt aagtccgctt
     2281 tctcataatc tgttgccctc tggggtctct aagtacgcta tcagatatcc ttgcgtccgg
     2341 cctctgaagg actctccgtg atacgtaacg ttaatgaccg gcattcggaa cgcacgcgtt
     2401 cgtcaacgcg ggcgtcagtg accgattgga ggtttatagc cacgttttca agagcgccac
     2461 gaaccgaggc agacgccatc agacgcgcta cacgaacgtc tgacaacata gatgggctgg
     2521 atagcgattc cagttggccg agccgctcaa ataattgcgc cgcttcgcgc gcgatttcga
     2581 gcggcgcttc gattgcgtcc aagagagcat tctgaatggc tgtgtggcgc tgctccctat
     2641 catcctccgt tccattcgga agcctgtaag cggccatcac ggattggaat gattcggcat
     2701 cgcggtcgat agcggctgcg agcctgcgag acgccgcatc gaattctttc actgcctcat
     2761 cgagccgctc tttatgcgcc gcgcccgatt tcttgatgct cgataagcca gccaccatct
     2821 gcccgaggct tgccgctaac gcacccgcta acgctgaagc ggaacctccg ccgggagttg
     2881 cagtcggctg cgccaaggct tccagaaacg gcgttacgat ggcggcgaga ctagcgttcg
     2941 cgccgctctc catcagtgat tcctccaatc gattctccaa cacttgcgcg ggcgaaaagt
     3001 tttcgacttt gaggtagttt tcggcggtca tttcaatcgc gcgcctggga atcaggccca
     3061 cgatctcgct tccgacgatt ccaactccca gtcgctcggc ctcgcgtttc accgtttcga
     3121 ataccagatg gatcggagtc tgttcgaagt cggtcaagtt catggaaact tgcgcgagat
     3181 tccgcgcgcg caaatccacg cccatggcct tgacgaatgg caagcccccg ctcgaaaaac
     3241 gaattgtttt ggcgattttc ttagcgatgg ccacatccgg agtattcaaa ttgatgttgt
     3301 aggcgatcag gaatttgcga gcacctacca cggtgccgcc agctgtggga tgcaggcgag
     3361 catccccgac gtcgggggca cgttctgggt tgcggaggat ttcctcgcgc aacccctcga
     3421 attggcctcg acgaatattc tctaaattca cgcgatcagg gcgctgcgcc gccgcctcgt
     3481 agaaaaaaac ggggatgcga tagcgctccc agatctcgcg tccggccttt ttcgcaagaa
     3541 tcacgcaatc ctctagagaa actccttcga gcggaataaa gggcaccaca tccgtcgcac
     3601 cgatgcgagg atgcgcacca gtttgccgcg tcaaatcaat cagttcggag gctttgccaa
     3661 cggctcgaac ggccgcttcc acaattccct cgggatcgcc tgccagagtg atcacgcttc
     3721 tgttatggtc cgcatcgctc tctcgagcca gcagaaagac ctcgggaact tcgcgtatag
     3781 cggctgcaat cgcgtcgact ttggatagat cgcggccttc tgcaaaattt ggaacgcatt
     3841 caacgagccg tttcatgtat tagtttttgt tcgggtggat caccacgccg cgcttcatcg
     3901 tcaggttgca gaggttcatg ccaaaataat acggaatctc gcggtaggag gctacgttaa
     3961 aggctgcaag gtccgcatat ttaccttcct caagcgagcc aattcggtcc tgttcgcgca
     4021 acgagtaggc ggcgttgatc gtggcggcgg aaatcgcttc cgcgggaata acgcgcattt
     4081 gagaacacgc cagcgagaga atcatcggca tgctcagcgt gggactcgtg cccgggttga
     4141 aatccgtcgc tagcgcaaca atggcaccag catcaatcag tttgcgcgcc ggagcgtaac
     4201 aaccggtgcc caggtgaaag ccacaaccc
//
LOCUS       DATNDT010000028         6803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_237878, whole genome shotgun sequence.
ACCESSION   DATNDT010000028 DATNDT010000000
VERSION     DATNDT010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6803
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237878"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1410)
                     /locus_tag="VN788_01005"
     CDS             complement(97..1410)
                     /locus_tag="VN788_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU19140.1"
                     /translation="MRDGGPAKGSGISHAWRALRHRNFRLYFGGQGISLIGTWMTRLA
                     TSWLVYRLTGSALLLGVVGFAGQIPTFLLAPFAGVWIDRLNRRQVLVITQILAMVQSL
                     ALAALTLTKHITVLEIIWLGAFQGAINAFDMPGRQAFLVQMVSDRADLGNAIALNSSM
                     VNMARLVGPSLAGAVIAISNEGYCFLIDGISYLAVIASLLAMRIDAKAIERSALSMLV
                     QLKEGWTYVSGFAPIRTILLLFAIVSLMGWPFTVLMPIFAAKVLKGGPHTLGFLMAAM
                     GVGALVSAVSLAARRTVLGLGRMIPLSAGAFGIGLIFFGLSRNLWISLALMLVCGFGL
                     MHQMAASNTIIQTIVEESKRGRVMSFYTMAFVGMAPFGSLLAGSLANLIGAPETVMLT
                     GTCCVVGAIWFFSHLRMIRQLIRPIYADLGILPPADAVIQDSVAR"
     gene            1653..2027
                     /locus_tag="VN788_01010"
     CDS             1653..2027
                     /locus_tag="VN788_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018970948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HXU19141.1"
                     /translation="MEETDIYSMIGEEGFTRLVAAFYRQVPHDDILSAMYPRNDLQGA
                     EQRLRDFLIYRFGGPQKYIDERGHPRLRARHFPFRINQAARDRWMHLMNEAFADAALP
                     TEAEQFLRAFFEHMSTFMINHP"
     gene            complement(2077..3045)
                     /locus_tag="VN788_01015"
     CDS             complement(2077..3045)
                     /locus_tag="VN788_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19142.1"
                     /translation="MKTLGRAAGVLIALIGVLAMGQPTRAASGKCDRACLISEMNNYL
                     AAVVAHDPSRVKFSPNVKFVENTMRLKPGEGLWKTATAAPKTFKIYVPDPVSEEVGFI
                     GVMTENDKPIELAVRLKVRGGKITEAEHLIARDLRPTSLPNLVRPRPGLLATVPPSER
                     TPRAQMIKDALSYYTAIVTSNGKAAPFADDCVRRENGMQTTGNPPPAKPGGFATMGAM
                     GCAAQLDTHVMSYIARIEPRRVEIADPETGLVFGLSQFRHPQKEKSVKIVGVPGVESV
                     PMNRDPWDSVNAHIFKIRDGKIHEIEAMGFPVPYNSKTGWEQTGSR"
     gene            3463..4041
                     /locus_tag="VN788_01020"
     CDS             3463..4041
                     /locus_tag="VN788_01020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU19143.1"
                     /translation="MVLDADVVQLRRGDLDALTALIARYQNRLYRYLLRLVREPAEAE
                     DLFQQSWLRVAEKIRSYDPSRSFDSWLFTIARNLALDHLRRVRPRSLDEPALRGSDSE
                     SLADRLPSGEILPLDRILEIERSGRLAAALLDLPASYREVLSLRFEEEMTLEEIAEVL
                     GAPLSTVKSRLRRSLEQLRSTLEGYNFRGDLS"
     gene            4038..4517
                     /locus_tag="VN788_01025"
     CDS             4038..4517
                     /locus_tag="VN788_01025"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXU19144.1"
                     /translation="MNPANDHEKLRSLLSLAASGALDPADEISVETHVRSCPECAAEL
                     EHWQALTGGLHRLPTPQLSPVVLQRTVALVREAQVARDERRATFKILAPILALSWLST
                     IFSWPLFELASEALRVLGIHFEPDWRVFAFFMIWTWLAAAVAAALLSSHQRHDRRLA"
     gene            4514..5038
                     /locus_tag="VN788_01030"
     CDS             4514..5038
                     /locus_tag="VN788_01030"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXU19145.1"
                     /translation="MTVIERKQRVIPKLAWFLLFFCWIALGTLIFEFALTDPEFANWP
                     AIGRIALAYGATLAGAAYVLLIVYVYGDAKRRGMRYVMWTLLAIFIPDAIGVILYFIL
                     RDPLPNPCPSCGKSVKASFTFCPYCSASLQHTCPQCGFAVDRAWLHCPKCGTKLPTAA
                     LQQTVSPTAPQSSF"
     gene            5490..6239
                     /locus_tag="VN788_01035"
     CDS             5490..6239
                     /locus_tag="VN788_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013312766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19146.1"
                     /translation="MQDKPVALVTGANKGIGLQIAKDLAAHGFTVLVGSRNLERGETA
                     AKSIGPDARALHLDVTNQASIAAAAERIKKELGRLDVLVNNAGISHTGKPGRPLEEIV
                     KSGRASVASLDEVRAVFETNVFGVIAVTQAMLPLLREAPAARIVNVSSGTGSLAMNSD
                     PTNPRRSMSGTYSWSKTALNAVTVGFAADLESAGIKVNAACPGFTATDLNNFQGTRTV
                     QQAAREPVRLALLDANGPTGTYSSDDGPVPW"
     gene            6478..>6803
                     /locus_tag="VN788_01040"
     CDS             6478..>6803
                     /locus_tag="VN788_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HXU19147.1"
                     /translation="MTGNEIRATFLRFFEERGHRVVRSSSLVPTNDPTLLFTNAGMNQ
                     FKDVFLGLEQRDYNRATSCQKCVRAGGKHNDLENVGFTNRHHTFFEMLGNFSFGDYFK
                     KDAIAYA"
BASE COUNT         1427 a         2270 c         1713 g         1393 t
ORIGIN      
        1 acaagtactt gcggccgtgc agactcccgc gcgaaaatat ctcttgtatc ctcgtagtgc
       61 gccctctcgc aggaaaatcc cttatgccgg ccattctcag cgcgccacgc tgtcctggat
      121 aactgcgtcg gctggcggca atatccccag atccgcatag atcggacgga taagctgccg
      181 aatcattcgc aaatgtgaaa agaaccaaat cgctccaacc acgcagcatg tcccggtcag
      241 catcacggtc tccggcgcgc cgatcaagtt agccaggctg cccgccagca aactcccaaa
      301 cggcgccatg cccacaaacg ccatggtata aaaactcatc acccggcctc gtttactttc
      361 ctcgacaatc gtttgaatga tggtattgct tgcggccatt tggtgcatca gcccaaaacc
      421 gcacaccaac atcagtgcaa gcgatatcca aaggtttcgc gatagcccga aaaagattag
      481 ccctattcca aatgctccgg cggaaagcgg aatcatcctg ccaagtccca gcactgttct
      541 tcgcgctgcc agcgacacgg ccgaaaccag cgctcccact cccatcgcag ccatcagaaa
      601 tcccagcgta tgcggacccc ccttcaaaac cttcgcggca aaaatcggca tcagcactgt
      661 gaacggccag cccatcaggc tcacgattgc aaacaaaagt aaaatagtgc gaatcggcgc
      721 gaatccggac acatacgtcc aaccctcctt caattgcacg agcattgaaa gcgcgctccg
      781 ctcgatggcc ttggcgtcga tcctcatcgc cagcagcgac gcaatcaccg caaggtaact
      841 gatcccgtca atcagaaaac agtagccttc attcgaaatc gcgatcaccg cccctgcgag
      901 cgacggccca acaagccgcg ccatatttac catcgatgaa ttcagcgcga tcgcgttgcc
      961 caggtctgct ctgtccgaca ccatttgcac cagaaacgcc tgccgccccg gcatatcgaa
     1021 cgcattgatc gccccctgaa atgcccccag ccagatgatt tcgagcacgg taatgtgctt
     1081 cgtaagcgtg agagcggcca acgcaagcga ctgcaccatc gccaaaattt gcgtaatcac
     1141 cagaacttgc cgccggttca gccggtcaat ccaaaccccc gcaaatggtg ccagaagaaa
     1201 ggtcggtatc tgcccggcga atcccaccac gcccagcagc agcgcggaac cggtcaaccg
     1261 gtaaaccagc cagctcgtcg ccagccgtgt catccatgtc ccaatcagcg aaatgccctg
     1321 cccgccaaag tacagccgga aatttcgatg ccgcaacgcc cgccaagcat gtgaaatccc
     1381 gctgcccttt gccggaccgc catcgcgcaa tctagccttc tccgaagtgt cctgaacact
     1441 cacaaatcca ttgtgccatt caccgcactt gaattcgacg ccgagtcgcc gtcattctga
     1501 gcggagtgtc ccgcggtttt ggttttgcgc gaagcgccgg gacacgcagt cgaagaatct
     1561 ctcttggatg ttcaccacgg cgcccgctcg ctcattcact gcctcccgcg aaaactgtcc
     1621 acttgcaaat gctaaactgg cgcaacgaac caatggaaga aactgacatc tattcgatga
     1681 ttggagagga aggcttcacc cgcctagtcg ccgccttcta ccgccaggtt ccacatgacg
     1741 atattctcag tgcgatgtat ccgcgcaacg atcttcaagg cgccgagcag cgccttcgcg
     1801 acttcctaat ttacaggttt ggaggacctc agaaatacat cgatgaacgc ggacatcccc
     1861 gccttcgcgc gcgccacttt cccttccgaa tcaatcaggc cgcccgcgac cgctggatgc
     1921 atttgatgaa cgaagccttt gccgatgccg ctctacccac agaagcagaa cagttccttc
     1981 gcgcattctt cgagcacatg tccaccttca tgatcaatca cccgtagtcg gagaaaagtc
     2041 gcgaagtgtg acttcagtct tggctcctcg cgcctttcat cgcgagccgg tttgttccca
     2101 gcctgtcttc gaattgtaag gaaccggaaa tcccatggct tcaatctcat gaatcttccc
     2161 gtctcgaatt ttgaaaatat gcgcgttcac actgtcccac ggatcgcgat tcatcggcac
     2221 cgattccacg ccgggcacgc ccacgatttt cacggatttc tccttctgcg ggtgacgaaa
     2281 ctgcgaaaga ccgaacacca aaccagtctc cgggtcggca atttccactc gccgtggctc
     2341 gatccgcgca atatagctca ttacgtgtgt atccagttgt gccgcgcagc ccatcgcgcc
     2401 catcgttgca aagccccccg gcttggccgg cggaggattg cccgtcgtct gcattccgtt
     2461 ctcgcgccgg acgcagtcgt ccgcaaacgg cgccgctttg ccattgctcg taacgatcgc
     2521 cgtgtaatat gacagcgcat ccttaatcat ctgcgctcga ggcgttcgct ccgaaggcgg
     2581 caccgtagcc agcaagccgg gccttggcct cacgaggttg ggtaaacttg tcggccggag
     2641 atcgcgagcg atcaggtgct cggcctccgt aatttttcca ccacggacct tcaggcgcac
     2701 agccaattca ataggcttgt cgttttccgt catcacgcca atgaacccaa cttcttccga
     2761 aactggatcg ggcacataaa ttttgaaagt ttttggtgcg gcggtcgccg tcttccaaag
     2821 cccctccccc ggcttcaacc gcattgtgtt ttccacaaac ttcacattcg gagaaaactt
     2881 cacgcgcgaa ggatcatgcg caacaacggc cgcgaggtaa ttattcatct cgctgatcaa
     2941 acaagcgcga tcgcatttcc ccgacgcagc gcgcgtaggc tgtcccatcg ccagcacgcc
     3001 gatcaaagca atcaggactc ctgctgctct acccaaagtt ttcactgtat tcccccatgc
     3061 tcctagccga aaagagcggc ggcaagcata acgccaatcg cgcggtattc gtaggggcac
     3121 ggcatgccgt gcccgcttgt cctgcgctgg gtggcgaaga ggcaatcggc gcctttgatt
     3181 tttatgtagc tcaccccgcc gtcacggggg tgaggatttt cgtccgcaca gcaccgtccc
     3241 caatcagcac cacacacttt tagggggtcg gaccttcagg tccgacataa accgacggcg
     3301 cgaagcgtct tcctctgcgc cgtttcaccc gcagcatgta atccttgtcg attacagggg
     3361 gtccaaatca gcgccacact cgcgcccttc ctgaatcctt ttggggggcc ttcccgtacc
     3421 tggaatatga tgagcaccgc aattcacgtc tgggagtctc cgatggtgct tgacgcagac
     3481 gtcgtgcagc tgcggcgcgg ggacctcgac gcgcttaccg cgctgatcgc gcgctatcaa
     3541 aaccgcctct accgctacct gttgcgcctc gtccgcgaac ccgccgaagc ggaggatctc
     3601 ttccaacaat cctggcttcg cgtggcggaa aaaattcgca gttacgaccc gagccggagc
     3661 ttcgattcct ggctcttcac cattgctcgc aatctggccc tcgaccatct tcgccgtgtc
     3721 cgcccgcgaa gcctcgacga acccgccttg agaggctccg attcagaatc tcttgccgat
     3781 cgccttccgt cgggcgaaat tcttccgctt gaccgcattt tggaaatcga gcgctccgga
     3841 aggctggcag ccgcactttt ggatttgccg gcaagttacc gcgaggtcct ctcgcttcgt
     3901 ttcgaagaag aaatgacact cgaggaaatt gcagaagttc ttggcgcgcc tctttccacg
     3961 gtaaaatcgc gcttgcgccg ctcccttgaa cagctcagaa gcacactgga aggctataac
     4021 ttccgtggag acctctcatg aaccctgcaa acgaccacga aaagcttcgt tccctgttgt
     4081 ccctggccgc ttcgggagcg ctcgatcccg ctgacgaaat cagtgtcgaa acccacgtgc
     4141 gatcctgccc cgaatgcgcc gccgagctcg aacactggca ggctctcacc ggcggtctgc
     4201 accgattgcc cactcctcag ctttcaccgg tcgtgttgca gcgaacggtg gcgcttgttc
     4261 gtgaagccca agttgccaga gacgaacgcc gcgctacttt caaaatcttg gcccctattc
     4321 tcgctctgtc ttggctctcg acgatcttca gttggccctt attcgagctc gcgagtgaag
     4381 ctctgcgagt cttgggcatc catttcgagc ccgactggcg cgtgtttgct ttctttatga
     4441 tctggacttg gctcgccgcg gccgtcgctg cggccctact ctcgtctcac caacggcacg
     4501 ataggagact cgcatgaccg tcatcgagcg caaacagcgt gtaatcccaa agctcgcctg
     4561 gttcttgctg ttcttctgct ggatcgcgct cggcactttg atctttgaat tcgcgctgac
     4621 cgatccggag tttgcaaact ggcccgctat cggcaggatc gctctcgcct acggcgccac
     4681 attggccgga gccgcatatg ttcttctcat cgtttacgtt tacggcgacg cgaagcgccg
     4741 cggcatgcga tatgtgatgt ggacgcttct cgccattttt attcccgacg ccatcggcgt
     4801 catcctctat ttcattctcc gcgatccgct ccccaatcct tgcccgtcgt gcggaaagtc
     4861 tgtgaaagcc tccttcacat tctgccccta ctgttccgct tccttgcagc acacgtgtcc
     4921 ccaatgcggc ttcgccgtcg atcgcgcctg gctgcactgc ccaaaatgcg gaaccaagct
     4981 ccccacagcc gctcttcaac agaccgtgag cccaacagcc ccacagtcgt cattctgaac
     5041 gaagtgtccc gcgccttctc ttcgcagtcg taggggcgca gcaccggagc cctcccgcgc
     5101 gctgctttcg cgtgctgggg tggtagcatt ctgtgcgcgg caaacatact tggctacccc
     5161 taccccgtcc acctcgctct caccggcgcc gtcattccgc ctgcttgcac tgagcgcagc
     5221 gaatgtgccc tgacgcagga aggaagcgga gtgtcccgcg cttttggttt tgcgcgaagc
     5281 gccgggacac gcagccgagg aatctctctt cgattcaata gcctgcgcga agggagccct
     5341 gacatagcga ctcgcgtcaa agcgcgatac gattgcgctg cctcacccgc agcaagtatt
     5401 cgagtttggg gccatcgccg ccacgtatcg cccatgcaca ggatcgccgc ggtgagccgg
     5461 cgcactaagc tgcaaaaaag gagatcacca tgcaggataa acccgtcgcc ctggtcaccg
     5521 gggccaacaa aggaatcggc cttcaaatcg ccaaggatct cgcggcgcac ggcttcaccg
     5581 tgctcgtcgg gtcgcgcaac ctcgagcgcg gagagacggc cgcgaaaagc atcggaccgg
     5641 acgcccgcgc tctccatctc gacgtcacga atcaagcctc cattgcagcc gccgcagagc
     5701 gcatcaagaa agaactcggc cgcctggacg tgctcgtgaa caatgcgggc atctcgcata
     5761 cgggaaagcc agggaggccg ctcgaggaga tagtcaagtc cggtcgcgcg agcgtcgcgt
     5821 cgctcgacga ggtgcgtgcg gtgttcgaga cgaacgtgtt cggcgtcatc gccgtcaccc
     5881 aggcgatgct gccactgctt cgtgaagcgc ctgcggcacg catcgtcaac gtatcgagcg
     5941 gcaccggctc gctcgcgatg aactcggatc cgacgaaccc gcgccgctcg atgtccggca
     6001 cctattcttg gtcgaagacg gcgctcaacg ctgtcacagt tggcttcgct gccgatctgg
     6061 agtcggccgg catcaaggtc aacgctgctt gccccggctt caccgcgacg gacctcaaca
     6121 acttccaggg cacgcgcacc gtccaacagg ccgcacgtga gcccgtacgc ctcgcgctcc
     6181 tcgacgcgaa cggtccaacc ggcacgtact cgagcgacga cggcccggtc ccttggtgat
     6241 tagtcgaact tcggacatgc aaaggtggaa gggcggggct tcagccgcgc catcaatcga
     6301 cggcgcgaag ccccttccgc tgcgctgcct cacccgcagc atgtagtcct acgctccaag
     6361 gcgagcgaat acaaagacac acggaccatc ccttgcgccg cctcgtttct ctccctaata
     6421 tggctgacgt gaaccatcgc gcactgtact cagcgcataa tcgaatccga ggaacaattg
     6481 accggtaacg aaatcagagc aaccttcctc cgcttcttcg aggaacgcgg ccatcgcgtc
     6541 gtgcgcagtt cctcgctcgt ccccacgaac gatcccacgc tgctcttcac caacgccggc
     6601 atgaaccaat tcaaggatgt tttcctcggc ctcgaacagc gcgattacaa ccgcgcaacc
     6661 tcctgccaga aatgcgtccg cgccggcggc aagcacaacg atctggaaaa tgtcggcttc
     6721 accaatcgcc atcacacttt tttcgaaatg ctcggcaatt tttccttcgg cgactatttc
     6781 aaaaaagacg ccatcgccta cgc
//
LOCUS       DATNDT010000029         5360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_234467, whole genome shotgun sequence.
ACCESSION   DATNDT010000029 DATNDT010000000
VERSION     DATNDT010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234467"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="VN788_01045"
     CDS             <1..512
                     /locus_tag="VN788_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXU19148.1"
                     /translation="GRGLSDPNTLRAGVGGTPLISVYAAGYRELEQQIGGPQSRVPAS
                     ANKIRNANAVGVEMQVAAGEEVRRLEKGMSTLATIASVSPFIGLFGTVWGVMDAFSGL
                     GDVGAASLRAVAPGISEALITTAAGLFAAIPALIAYNHYLHGIRAVASRMSDFAAEFV
                     AKVETLYSS"
     gene            519..941
                     /locus_tag="VN788_01050"
     CDS             519..941
                     /locus_tag="VN788_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU19149.1"
                     /translation="MPQAQREVGTSLSDINMVPFIDVVLVLLIIFMITAPILQSGIDV
                     DVPKTKTVKELTEVRMVVTIDRGQRVYLNDKPVNIHEIGQKVVSQSRDPKSQSVYVRC
                     DETVPFGSWATVVDALRQSGITNISVVTQPISERPQTQ"
     gene            943..1731
                     /locus_tag="VN788_01055"
     CDS             943..1731
                     /locus_tag="VN788_01055"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXU19150.1"
                     /translation="MAAIAAIRPDPTLKRFIYASLGLHCILVAWIVVPAVFTGHHEDW
                     GGPGGSVTVNVVGSVPAIPLPQPDVESMNRVVDNSKGLFQTEPKPIPKPEPDATPIPK
                     FQKTKPPKYITKPSKVLENPTPPPPNAVPYGGGGTPTVPRTSFAMGSGTTQASLGFTG
                     TAGGDFGSRFSWYVEAVQRRISGNWLQSSVDPSVSTAPRVVVTFTILRDGTVTNAQIT
                     HSSNNYSVDNSALRAVRDSSPLDRLPGGYQGPSVNVEFWFDFHR"
     gene            1760..3106
                     /locus_tag="VN788_01060"
     CDS             1760..3106
                     /locus_tag="VN788_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HXU19151.1"
                     /translation="MKRQTAFFLISALIAACVTILSAPPAAAQDWFKTGTGLGVTKAR
                     LAVPDEGSRSTFAQPLPKTFHDVLWADLQYSGVLDLVSPSFYPLQVPTQPSELKAQDW
                     SAAPANAYMVAYGSLSLSGTSLSFAGFLSDVRNPTAPLALQKVYTGMATNEGARTLAH
                     QFANDIVAVLSGGQPGIALTRISYVSSRSGNKEIWEMDYDGANAHQLTHLKTIALTPR
                     WSPDAERIAFTCYVPVRGVTSAQICIYSLASNRMISFPRYRGTNSSPAWSPDGSQIAF
                     MSSQNGDPEIYVTNANGGRSQRITFARGVNTSPAWNPKTGKQIVFVSDRGGNPDLYLA
                     NSDGSNVERINLPDMGYVIDPAWSPNGQLLAFSWRRPSGNYDIYAMDIISHQLVELTR
                     DAGRNERPSWAPDGRHLVFESTRTGTRQIWSMLADGSQPRQLTFEGQSESPNWSPK"
     gene            3225..3968
                     /gene="pal"
                     /locus_tag="VN788_01065"
     CDS             3225..3968
                     /gene="pal"
                     /locus_tag="VN788_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HXU19152.1"
                     /translation="MQHGSFRRIALSLGLVAFAAVAGGCKKNVASTPPAPAPAPAAAE
                     PTVTLNASPATVNPGEAVTLSWTSTNATDLDIQPGVGSVAPQGMRGVNPTQSTTYTIT
                     ATGAGGSATATAHVDVNTGQAQQPAAQQSVSELFGQNVKDAYFDTDKSDLGSEARSVL
                     TKDAEFFRSYPQVRVSIEGDCDERGSTEYNLGLGQRRAEAAKNFLISLGITADRMETT
                     SWGKERPVCSEHTEQCWQLNRRAHFVLTQ"
     gene            4155..5108
                     /locus_tag="VN788_01070"
     CDS             4155..5108
                     /locus_tag="VN788_01070"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU19153.1"
                     /translation="MRTRWVVLTFAAVLLGAIGGAMLAPQPASAVSRDMIELQQQVAQ
                     LLQGQQDMRSAMDSNNATIKTLVQQSLDSVNKLSAEMSSVQKSVQEVQANTGARIDTM
                     AQQTQGLSDNLQDVQARVGKLSQQLSDAQNLLQSIDGKLSNQPGATGAPGATGAPGSP
                     GATGGPTTSNDTPGGNPPPGMAPISADTLYQNALRDYTSGKYDLARQEFSDYIKNFPS
                     NDLASNSQFYLGEIAYAQGDFKQAVGAYNAVLVNYPRSFKLAASQLKKGMAELESGER
                     TAGIRDLRTVESHFPGSDESRRAHAKLREVGATTTSRAPAR"
     gene            complement(5151..>5360)
                     /locus_tag="VN788_01075"
     CDS             complement(5151..>5360)
                     /locus_tag="VN788_01075"
                     /inference="COORDINATES: protein motif:HMM:NF040124.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6526 family protein"
                     /protein_id="HXU19154.1"
                     /translation="EMRLRMQQLLPPDLTPRIPEFTVSQLVALRFASDAELPDLARRV
                     LQENLTERKAIKAMVRNWQPDHLRA"
BASE COUNT         1180 a         1540 c         1601 g         1039 t
ORIGIN      
        1 cgggacgcgg cctatccgat cccaacacac ttcgggcagg ggtgggcggc acgccgctca
       61 tctcggtcta tgcggcaggc tatcgggaac tcgagcagca gattggcgga ccgcagtcac
      121 gcgtgcccgc tagcgcaaac aagatcagaa atgccaacgc agtaggcgtc gagatgcagg
      181 tggccgcggg tgaagaggtt cgacgccttg aaaaggggat gtcgacgctg gctaccatcg
      241 cctcagtgtc acccttcatt gggctattcg ggacggtctg gggtgtaatg gacgcatttt
      301 cgggcctggg cgatgtgggc gcggcaagtt tgcgcgcagt ggcgccaggc atctccgagg
      361 cactgatcac cacggcggcg ggattgttcg ccgcaattcc cgcgctgatc gcttacaacc
      421 actatttgca cgggattcgc gccgtcgctt cccgaatgtc cgatttcgcc gcggaatttg
      481 tggcgaaagt cgagacgctg tacagttcgt agacgctcat gcctcaagcc cagcgagaag
      541 tcggcacctc cctatccgac atcaacatgg tgccgtttat cgatgtggtt ttggtgctgc
      601 tgattatttt catgatcact gcgccgattc tgcagtccgg catcgatgtg gacgtgccca
      661 aaacaaagac ggtgaaagag ctgaccgagg tgcggatggt agtgaccatc gatcgcgggc
      721 agcgagtcta cctgaacgat aagccggtga acatccacga gatcgggcaa aaggtagtat
      781 cgcaatcgcg cgacccgaag agccagtccg tatatgttcg gtgtgacgaa acggtgcctt
      841 ttgggagctg ggcaaccgta gtggatgcgc tacggcagtc gggcataaca aatatcagcg
      901 tagtgacgca accaatcagc gagcgcccgc aaacacaatg acatggcagc catcgccgca
      961 atccggccgg acccgactct caaacgattt atctacgcat ccctcggatt acactgcatc
     1021 cttgttgcct ggatcgtggt gccggcggta tttaccggcc atcacgagga ctggggcgga
     1081 ccgggagggt cggtgaccgt caatgtggtg ggcagcgttc cggcgattcc gcttcctcag
     1141 cccgacgtgg aatcgatgaa tcgcgtagtg gataattcaa aaggtttatt tcagaccgag
     1201 cccaagccaa tacctaaacc ggaacccgac gccacaccga taccgaaatt tcaaaagacc
     1261 aagccgccga aatacataac caagccgtcg aaggtgctgg agaatcccac gccgccgcca
     1321 cccaatgcgg ttccctacgg tggaggcggc acgccaaccg ttccgaggac gtctttcgcg
     1381 atgggcagtg gcaccacgca ggcgagcttg gggttcacgg gcacggcggg cggagatttt
     1441 ggctcgcgct tttcgtggta cgtagaagca gtccagcggc ggatcagcgg caattggctc
     1501 caatccagcg tggatccgag cgtaagcacg gcgccgcgcg tggtggtgac gttcaccatc
     1561 ctgcgcgacg gcaccgtgac caacgcccag atcacgcact cgagcaacaa ctattccgtc
     1621 gataattcgg cgttgcgcgc cgtgcgcgat tcgagtccac tcgaccggct gcctggcggg
     1681 tatcaaggac cgagtgtgaa tgttgagttc tggttcgatt ttcatcgtta acaaaatacg
     1741 taacaaaaaa ggcaattata tgaaacgaca aaccgcgttt ttcctgattt cggctttgat
     1801 agctgcgtgc gtcaccattc tttccgcgcc acccgcagcc gcgcaagatt ggttcaagac
     1861 aggcaccggg ttgggggtaa ccaaagcccg attggccgtg cctgatgagg gcagccggtc
     1921 aacctttgcg cagccgctgc cgaagacctt tcacgacgta ctctgggccg atctgcaata
     1981 ttctggtgtt ctcgatcttg taagtcccag cttctacccc ttgcaggttc cgactcagcc
     2041 gagcgaattg aaggcgcagg attggtccgc cgctccggca aacgcgtaca tggtggctta
     2101 tggaagtcta agcctgagcg gcacgagcct ttctttcgcg ggatttcttt ctgacgttcg
     2161 caatcccacc gcgccgctcg cgctgcagaa agtttatacc ggaatggcga cgaacgaagg
     2221 tgcacgaaca ctggcgcatc agtttgccaa cgatatcgtg gccgttttga gcggggggca
     2281 gcccggcatc gcgcttacgc gcatcagcta cgtgagctcg cgatcaggca ataaggaaat
     2341 ctgggaaatg gattacgacg gcgcgaatgc gcaccaattg acgcacctga aaaccatcgc
     2401 gctcactccg cgctggtcgc cggatgcaga acgcattgcg ttcacgtgct acgttccggt
     2461 tcgcggagtg acatcggcgc aaatctgcat ctactcgctg gcatcgaacc ggatgatttc
     2521 cttcccgcgc tatcgaggaa cgaacagctc gccggcatgg tcgccggatg gttcgcaaat
     2581 cgccttcatg tccagccaga atggcgatcc tgaaatttac gtgacgaatg cgaacggagg
     2641 ccgttcgcaa cgaatcacat ttgcccgcgg ggtgaacacc tcgccggcgt ggaaccccaa
     2701 gaccggcaaa caaattgtct tcgtgagcga ccgcggaggc aatcccgatc tttaccttgc
     2761 taactcggac ggatcgaacg tggagagaat caaccttccg gacatgggat acgtgatcga
     2821 tccggcgtgg tcaccaaatg gacaactgct cgcatttagc tggcgacggc cgagtgggaa
     2881 ttacgatatc tacgctatgg acataattag tcaccaactg gtggagctta ctcgcgatgc
     2941 gggtcggaat gaacggccga gttgggcgcc ggacggccgt catttagttt tcgaatcgac
     3001 gcgaacgggg acgaggcaaa tctggtcgat gcttgcggat ggaagccagc cgaggcagct
     3061 gaccttcgaa ggacagagcg agtcgcctaa ttggtcccct aaatgaagta tttgaaactt
     3121 ttggttggat ataggggcgc catctgaaaa gcgtgcggtc aaacagctca attcaaatcg
     3181 cggcggttcc gaagccctca gaggaacgcc gaaggaggca gttcatgcag catggcagtt
     3241 ttcggaggat tgcgttatcg cttggattgg tagcttttgc cgctgttgcg ggtggttgca
     3301 aaaagaacgt agcgtcgact ccgccggcgc cagcgccggc gcccgcagcg gcagaaccca
     3361 ccgtaacgct caacgcgtcg cccgcaactg tgaatcctgg cgaagccgtg acgctctcat
     3421 ggacgtccac gaacgccacg gacctcgaca tccagcccgg cgtaggaagc gtggcgccac
     3481 aagggatgcg aggagtaaac ccgacgcaat cgacgacata cacgattacg gccacgggtg
     3541 cgggcggtag cgccacggcg acggcgcatg tggacgtaaa taccgggcaa gcgcagcaac
     3601 ctgccgctca gcaaagcgtg agcgagctgt ttggtcagaa cgtcaaagac gcatactttg
     3661 acacggataa atcggacctc ggcagcgagg ctcgcagcgt tctcaccaag gacgcggaat
     3721 tcttccgatc ttacccgcaa gtccgcgtct cgatcgaagg tgattgcgac gagcgcggct
     3781 caaccgaata caatcttggt ctaggccagc ggcgcgccga ggcggcaaag aacttcttga
     3841 tctcgctcgg catcaccgcc gatcggatgg aaacaaccag ctggggcaag gagcgtccgg
     3901 tttgcagcga gcacacggaa cagtgctggc aattgaaccg gcgcgcgcat ttcgttttaa
     3961 ctcaataggg tgcggcaatt gtggccgctg cccgggccac tgctacgtga tttgccgcgg
     4021 agccaccttt ttagtaccgc atgggcgcgg caacgttaat aatatagaga agggtttgct
     4081 acctcgcgga ttgcggggcg gtgcaaatcg gcctggccga atcggccggg tgaatttttc
     4141 cgggaggata ccctatgcgc acacgatggg ttgtgctcac cttcgcagcg gtgctgttag
     4201 gcgctatcgg cggggcgatg ttggctccgc agcccgccag tgccgtttca cgcgatatga
     4261 tcgagcttca gcagcaggtt gcccaacttc tgcagggaca acaggacatg cgcagcgcga
     4321 tggatagcaa caacgcaacc atcaagacgc tggtacagca atcgctcgat tccgtcaaca
     4381 agctcagcgc tgaaatgagc tcggtgcaaa agtccgtgca agaggtgcag gcgaatacgg
     4441 gggcccgcat tgacacgatg gcccagcaga cccagggact ctccgacaat ttgcaagacg
     4501 tgcaggcgcg cgttggcaaa ttatcgcaac aactctccga tgcgcagaac ctgcttcaga
     4561 gcatcgacgg gaaactgtcg aatcagccgg gcgcgaccgg ggcgccgggc gcgactgggg
     4621 cgccgggctc gccaggcgcg acgggagggc ccacaacttc gaatgacacg cccggtggaa
     4681 acccgccgcc aggaatggcc ccgatctcgg ccgatacgct ttatcaaaac gcgctgaggg
     4741 actacaccag cggcaagtac gatctggcgc gccaggagtt ttccgactac atcaaaaatt
     4801 tcccctcgaa cgatctggcc tccaactcgc agttttatct aggcgaaatt gcttacgcgc
     4861 aaggggattt caagcaagca gttggcgcct acaacgcggt gctggtgaat tatccgagga
     4921 gtttcaaatt ggcagcatcg cagctcaaga aagggatggc ggaacttgaa tccggcgagc
     4981 gaacggccgg cattcgtgat ctgcgaaccg tcgagagtca cttccccggc tcggacgaat
     5041 cgcggcgcgc tcacgccaaa ctgcgcgagg tcggcgccac cacaacttcg cgggctcctg
     5101 cgcgctaatc cccaaggcct cggcgttccg ttctagcgtt gactgcccct ctatgcccgc
     5161 aagtggtcag gctgccaatt tcgaaccatt gctttgatcg ctttgcgctc cgtcaaattc
     5221 tcctgcagaa ctcttcgcgc gagatccggc agttccgcgt cgctcgcgaa tcgcagagcc
     5281 accagctggc tcactgtgaa ttcgggaatt cgcggcgtta agtccggtgg aagcaactgc
     5341 tgcatgcgca atcgcatttc
//
LOCUS       DATNDT010000030         8754 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2816506, whole genome shotgun sequence.
ACCESSION   DATNDT010000030 DATNDT010000000
VERSION     DATNDT010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8754
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2816506"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="VN788_01080"
     CDS             <1..375
                     /locus_tag="VN788_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXU19155.1"
                     /translation="EVSRLTATKTFVLRYWESEFPTLQPVKSPSGHRLYRREDIDTVF
                     EIKRLLYEQGFTIAGARKHLAEQAGQNGVDVSHHDMHSAAAAADPPALPAQAKPASRV
                     DRKLLLDLHEELRAILTLLERE"
     gene            425..501
                     /locus_tag="VN788_01085"
     tRNA            425..501
                     /locus_tag="VN788_01085"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:459..461,aa:Pro,seq:ggg)
     gene            562..1314
                     /gene="surE"
                     /locus_tag="VN788_01090"
     CDS             562..1314
                     /gene="surE"
                     /locus_tag="VN788_01090"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HXU19156.1"
                     /translation="MSNILITNDDGIHAPGLRALAAALNDLGTITIVAPSAERSASSQ
                     ALTLRQPIYCDQLAEREYAVEGTPADAMILAFHTLLKERPDLALSGINPGANLGENIY
                     YSGTVGAAMEAAINRTPAIAISVAYKGRGFDFAPTSRFARRLAPLILREGLPAGVMLN
                     VNVPQDWTGEVRFTRQSSKITRNMLQPGTDPRGRHYYWLSEQQFVEGIEPDTDQAAIR
                     DGAASITPLQIDHTHAPSLNRFSHWVKCLADC"
     gene            1644..4169
                     /locus_tag="VN788_01095"
     CDS             1644..4169
                     /locus_tag="VN788_01095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19157.1"
                     /translation="MNGNRPESVVGAGFREMEAEIELRVGRRTSQLRAAYNRLESVIN
                     AAKLTSIISIDLQGIITVFNSGAERMLGYSASEMVGKQSPVIFHLPSEIEERARSLSE
                     QFGRPVKGVEIFVGLAREGEYEEREWTYVRKDGGRLIVSIVVTAVQDAKGEITGYLGV
                     AQDVTERREAESRLLLLTERLSLATDVAALGVWEWDVANNGMAWDTTMVQIYGFPLDP
                     ANPYKQWSSAVYPDDLPAAEGALKKVMDTKGRASVEFRIIRPDGAIRYLAAAEGVVLD
                     ENGNVSRIIGINIDITDRKQAETDLQRAKEGAEAANRAKSEFLANMSHEIRTPMNGIM
                     GMTDLVLDSELNEEQREYLATVKTSADSLLTVINDILDFSKIEAGKIDIESLNFNLHD
                     TLETTLKTLALRAHEKGLELVCEIAPEVPEAVRGDAARLRQVVVNLLGNAIKFTQSGE
                     VSLNVHLESREKDECMLHFTVSDTGIGIPSEKLKVIFDPFSQADTSTTRHYGGTGLGL
                     TISTRLIEKMGGIVWVESEVGRGTQFHFTIRLGAIDGTGIAIGTIAPPEILHGVKVLV
                     VDDNKTNRRILEGMLKRWEMKSTSVAGGEEALAQLSAAWEVKQPFGLILMDMHMPQMD
                     GFGLVERIRGRPELSTATIMMLTSAGHRGDGQRCKELGVSAYLLKPIRQSELREAIAR
                     VLGAREQTGAIPLITRYSLADAREPGASLQILVAEDNAVNQLLVRRMLEKRGHRVSVV
                     NNGREVLKAIENESYDLVLMDVQMPEMDGIEATLAIREKEKEQDNQSHQAIVALTAHA
                     MKGDQERCQAAGMDDYLTKPIRPQELDKLLERYAAAKMNATTP"
     gene            complement(4432..4917)
                     /locus_tag="VN788_01100"
     CDS             complement(4432..4917)
                     /locus_tag="VN788_01100"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU19158.1"
                     /translation="MSHWIGTTSPADAGMSDFKECLKHLRDGDPSEALLHARRALGIA
                     PKNPFYLSYTGLLAAVAERRYGDGEALCQEALGMRHNHAQLYLNLAEVYQQSGRTPDA
                     IETLEKGLVSAGRDFRIRRALQKIGTRREPLLAFLHRSHPLNRALGKWRHRITGPSQA
                     A"
     gene            complement(5137..6345)
                     /locus_tag="VN788_01105"
     CDS             complement(5137..6345)
                     /locus_tag="VN788_01105"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXU19159.1"
                     /translation="MSSLTKKIVIALSAVVFLYVTSGYVMGRSSDDKAFRALTVYSEV
                     LEHIQRDYVDDPNMHQVTSGALHGLLDSLDSQSSYLSPLEYKDFEEKSAANSKAGAGL
                     ALTKRFGYISVISALPDSPAKKAGLGLGDVLEKIAGFTTGQMAVEQAQLLLSGEPGAV
                     VKLSVIRRGKTEPEDVDLTLEKLPAPKLVEDKLEGDIAYLHVPEFDAGETKRIRDELV
                     QFQRQGAHKLILDLRDCAVGDDQEGISTAQLFLSSGTIATLKGQTVAPVVSTADPSKV
                     VWTQPLTVLIGNGTAGPAEIVAAAIADNHRGETIGDRTYGIASEQKLIPLDDGSALIL
                     TVATYYEPSGKEIPVEGVAPTRQVRPVPDDVADITDQNLGAPSSSPSDPVIKKALEVL
                     QTASPARKAA"
     gene            complement(6503..7384)
                     /locus_tag="VN788_01110"
     CDS             complement(6503..7384)
                     /locus_tag="VN788_01110"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXU19160.1"
                     /translation="MLNDTESCSFCDVSPSENEEVYESVAVGAVHLAESAEPEWRTEV
                     SRRLEEYRTRRGRLRPDLPDTQPGLPFKKPAPTHATLDLDDEVRERPPQRPAPRLRQK
                     PPQTERVEIRVQPELDFSSSPYDRARPQTALVPVAPLAHRRSACYIDFCFLALTCAGF
                     VGLFLFASRSAGGELTLGKSDAIAYVAIAYLFYALYLSLFVILAGSTPGMQLRGLSIV
                     RLDGSLPDTRQLLWRVFGYLLSGATLMIGFFWALWDEDHFTWHDRISQTYITAAMPLA
                     DADSYSVPSGRQTFAHK"
     gene            complement(7543..8523)
                     /locus_tag="VN788_01115"
     CDS             complement(7543..8523)
                     /locus_tag="VN788_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXU19161.1"
                     /translation="MSEASPAKSGLFDPSVFKPQAVSAETLVANEAFRKALSGGPDWW
                     DIGAPAFREAAAAGRGPFPPPEKSARARTIQIDGKGGHKVPVRVIAPERAKGVYIHIH
                     GGGLVFGSSEGQDPMLERIVQSTGMACVSVEYRLAPENPYPAAWDDCESVALWLTKNA
                     KSEFGADVLSIGGESAGATLTAATLLRMRDRHGYTGFRAAVLSYGNFDSSMTPSQTLT
                     PDRGILVGKISIHKFREAYLPAGVNPRDPDVSPLYADLKNMPPAIFTVGTIDPLLDDT
                     LFLYARWIAAGNQAELDVYPGAPHAFNIFPLPLGTPANARIDDFLKRAIS"
BASE COUNT         1906 a         2357 c         2757 g         1734 t
ORIGIN      
        1 gaagtgagcc ggttgacggc gaccaagacg tttgtcttgc gctactggga gtctgagttt
       61 cccacgcttc aaccggtaaa aagtcccagt gggcaccggt tatatcggcg cgaagacatc
      121 gataccgtct tcgagattaa acggttgctt tacgaacaag gttttacgat cgcgggcgcc
      181 cgtaagcatc ttgccgagca agcggggcag aacggcgtcg atgtttcgca ccatgacatg
      241 cactcggctg cggcggccgc ggaccctccg gcgctgccag cccaggcaaa gccggcatcg
      301 cgcgtagacc ggaaactcct gctggacctt cacgaagagc ttcgcgcgat cttgaccctt
      361 ctcgaacgcg agtgatacgc tcgcagaagc cctttacaat tgggcaatgt ggtgtcagtg
      421 cagtcgggac gtggcgcagc ctggtagcgc gcacgcttgg ggtgcgtgag gtcgctggtt
      481 caaatccagt cgtcccgacc attgttttct tgccttgaaa gcgacagttt ttgggcagcg
      541 cttcacccgc gacggtaatc cgtgtcgaat attctgatca ctaatgacga tggcattcat
      601 gcgccggggt tgcgtgcgct ggctgccgcg ctgaacgacc tggggacgat cacgatcgtg
      661 gcgccttccg ccgaacgcag cgcctcctcg caggcgctga cccttcggca gcctatttac
      721 tgcgatcagc tcgccgagcg cgaatatgcg gtggaaggaa cgcccgccga cgcgatgatt
      781 ttagcgtttc acacgctttt gaaagaaagg cctgatctgg cgctttcggg catcaacccc
      841 ggtgcgaacc tgggcgagaa tatttattat tccggtacgg tgggtgctgc catggaagcg
      901 gcgatcaatc gcacgccagc gatcgctatt tccgtggcgt ataaaggaag gggtttcgat
      961 tttgcgccaa cctcgcgttt cgcgcgccgg ctcgcgccgc tgattctacg cgaaggattg
     1021 cctgctggag tgatgctcaa cgtcaatgtg ccgcaggact ggaccggcga ggtgcgtttc
     1081 acccgacaat cgtcgaaaat cacgcgcaat atgcttcagc ccggcaccga tccccgcggg
     1141 cggcactact actggctaag cgaacagcaa ttcgtcgagg ggattgaacc cgataccgac
     1201 caggccgcta ttcgcgacgg ggctgcctca atcacgccgc tgcaaatcga tcatacgcac
     1261 gctccgtcgc tgaaccgttt ctctcactgg gtgaaatgcc ttgccgactg ctgaggggcg
     1321 aaaggcgatg agcctttgcc tgtacccgac cagcccatcc tagagttttc ccaatcgcca
     1381 atctgaacga ctagcgaacc gtccaagtca tgaaagcatg tgtcgaggtc gattctaacc
     1441 cccgattcca agctcttggt ctctttaacc agtctggcgg caaggtaaac caaatcctgt
     1501 tcggcgccga tatttaggat atgacggctt ctgcgagcga gtgtaccaag acgatactgg
     1561 tctggtaccg cagcgggcag caggcgccaa agcgcgaacc gcaccgattc taaaatgcca
     1621 aaagcaacga ggttgaagca gcagtgaacg ggaaccgccc tgagagcgtt gtcggtgcgg
     1681 gtttccgtga gatggaagcc gaaatcgagc tgcgcgtcgg gcgccgcacg agccagcttc
     1741 gcgcggccta taaccggctc gaaagcgtga ttaacgcggc gaagctgacc tccattattt
     1801 ccatcgattt acagggcatc attacggtgt tcaactcagg cgcggagcga atgctgggct
     1861 attcggcaag cgaaatggtc gggaaacaga gtccagtgat tttccatctg ccatccgaga
     1921 tcgaagagcg ggcccgtagc ttgagcgaac aattcgggcg gcccgtcaaa ggcgttgaga
     1981 tatttgtagg acttgcgcga gaaggcgagt acgaggagcg ggaatggacg tacgtgcgga
     2041 aggatggcgg ccgtctgatc gtcagcatcg tggttacagc ggtccaggat gccaaagggg
     2101 aaattacggg atatctgggt gtggcccagg acgttacgga gcgccgagag gccgaatcca
     2161 gactgctatt actgacggaa cggctatcgt tggcgaccga tgttgcggcg ttgggcgtgt
     2221 gggaatggga cgtggcgaac aacgggatgg cttgggatac gaccatggtt cagatctacg
     2281 gcttccccct cgatccagca aatccctaca agcagtggag ctccgcagtt tacccggatg
     2341 atttgcctgc cgcggaaggc gccttgaaaa aggtgatgga caccaaggga agagcgtcgg
     2401 tggagttccg catcattcgg cccgatggcg cgatcagata tttggccgcc gccgagggag
     2461 tggtcctcga tgaaaacgga aacgtcagcc ggatcatcgg aataaatatc gatattaccg
     2521 atcgcaagca agcagagacc gatttgcaac gggccaaaga aggcgctgag gcagcgaatc
     2581 gcgcaaagag cgagttcctg gccaacatga gccatgaaat tcgcactccc atgaatggga
     2641 tcatggggat gacggacctg gtgctcgact ccgagctcaa cgaggagcag cgcgagtatt
     2701 tggccactgt gaaaacatcg gcggattcgc tgttgacggt gatcaacgac attctggact
     2761 tctccaagat cgaagcgggc aaaatcgaca ttgagtcgct caatttcaac ttgcacgaca
     2821 cgctcgaaac gacgctgaag acactggccc tgcgcgctca tgaaaaaggg ctcgaactgg
     2881 tgtgcgagat cgcgccggaa gttccggaag cggtgcgcgg agacgccgcc cggctgcgcc
     2941 aagtggttgt gaatctctta ggcaacgcca ttaaattcac gcagtcaggc gaagtgtcgt
     3001 tgaacgtcca tctggaaagc agggagaagg atgagtgcat gctgcacttc acggtttcgg
     3061 atacgggcat cgggattccg tccgaaaagc tgaaggtgat ttttgacccg ttttcgcagg
     3121 cggacacctc gactacgcgg cattacggcg gcacaggctt gggactgacc atctcgacgc
     3181 gcttgattga aaagatgggc ggaatcgtgt gggtggaaag cgaagtcggg cgtggcacgc
     3241 agtttcactt cacaattcgg ctgggggcca tcgatggaac gggaatcgct ataggaacga
     3301 ttgctccgcc ggaaatcttg cacggcgtaa aggtgttggt cgtcgatgac aataaaacca
     3361 accggcgaat actcgagggg atgctgaagc gctgggagat gaagagcaca tcagtggcag
     3421 gcggcgaaga ggccctagcg caactatccg cggcgtggga agtcaaacag ccgtttgggc
     3481 tgatcctcat ggacatgcac atgccccaaa tggacgggtt cgggctcgtc gaacgcattc
     3541 gcgggcggcc cgagttatcc accgccacga tcatgatgct tacatcggcc gggcataggg
     3601 gcgatggaca acgatgcaag gaactgggag tgtccgcgta cctattgaag cccatccggc
     3661 agtccgaatt gcgagaggcc attgcgcgag tgctgggagc tcgcgagcaa accggagcga
     3721 ttcctctgat cacgcgttat tcgctcgcgg acgctcgcga accaggagct tcgctgcaaa
     3781 tcctagttgc agaggacaat gccgtgaacc aactcttagt ccgtcgcatg ctggagaaga
     3841 gaggacatcg agtatcagtg gttaacaatg ggcgcgaagt cctgaaggcc atcgaaaacg
     3901 agagttacga cctggtcctg atggacgtgc agatgcctga aatggatggg atcgaggcga
     3961 ctctagccat ccgagagaag gagaaagaac aagacaacca aagccaccaa gcaatcgtcg
     4021 ccctgaccgc gcatgccatg aaaggtgatc aggagcgctg ccaagcagcg ggcatggacg
     4081 attacctcac caagcccatt cgccctcagg agttggacaa gctgttggag cgctatgcgg
     4141 cggcaaaaat gaacgccacc acgccctaac tttcctcaat cccgggaaaa cagacgaatt
     4201 aatcgagtgc ccatagcgaa ctacgcgaag gctggcgcta gccgacgcgc gagtcagcgg
     4261 cgtttcttgc catcggaggt ggatgacttt ctatcgagcc ggtgattttg cgggcgaacg
     4321 taatggggaa gtccgggatg gtcgccaact gccgttggga gtggcagcga gttggtagcg
     4381 catcccgggc tttgcccatg gagggaagcc tgtcggtggc ggtggcgttg ctcacgcagc
     4441 ctgcgagggg ccggtgatgc gatgcctcca ttttcctaac gcacgattca aggggtggct
     4501 gcggtgcagg aacgcgagca aaggctcgcg ccgtgtaccg attttttgca gcgcacggcg
     4561 aatgcggaaa tcgcggcccg cggacactaa tcctttttca agcgtctcaa ttgcgtccgg
     4621 cgtccggccg ctctgctggt acacctccgc gagattcagg tagagctggg cgtgattgtg
     4681 cctcatccca agggcttcct ggcacagcgc ttcaccatcg ccgtagcgcc tctcggcaac
     4741 agctgcgagc agtcccgtgt aagaaagata gaaggggttt ttgggagcta tacccagggc
     4801 gcgacgggcg tggaggaggg cttcgctggg gtctccatca cgcaggtgtt tcaggcactc
     4861 cttgaaatcc gacatgccgg catcagcggg ggaggtggtt ccgatccaat gtgacatggg
     4921 ttccttcctt gccctgcgga acagcggttg tagggccaga agtaacctaa cgcgatcgct
     4981 aagggagata aataggtcgt tggaccagat ttcgttccag tccttccgac tgtaccaagt
     5041 gacaggcgaa gaaatagcta tagacgagcg ggagtatcga ggacgctttt tccgacgagc
     5101 cttacccaag gctcccagat ggggagggtc cctgatctag gcggctttgc gagccgggga
     5161 agcggtctgc agaacctcaa gggccttctt gatgaccggg tcgctggggg agctggatgg
     5221 agcgcccagg ttctgatcgg tgatgtcagc gacatcgtcc ggtacgggac ggacctggcg
     5281 ggttggcgca actccttcga ccggaatttc cttccccgaa ggctcgtagt acgtggccac
     5341 ggtaagaatg agagctgagc catcatcgag tggaatcagc ttttgctcgg aagcaatgcc
     5401 gtaggtgcgg tcgccaatgg tttcaccgcg atgattatcc gcgatagcgg cggcgacgat
     5461 ttcagcagga ccagcggtgc cgttgccaat cagcacggtg aggggctggg tccatacaac
     5521 cttggagggg tcagcggtag aaaccacagg ggcgacggtt tgtcccttca gggtagctat
     5581 ggtgccggag gagagaaata gctgtgcggt ggaaatgcct tcctggtcgt caccaaccgc
     5641 acaatcgcgc aagtcgagga tcagtttgtg ggcaccctgg cgctgaaatt ggacgagttc
     5701 gtccctgatg cgctttgttt cgccagcatc gaattcggga acgtgcagat aggcgatgtc
     5761 accttcgagt ttgtcctcca caagcttggg agccggaagc ttttccagag ttagatctac
     5821 atcttcaggt tcggttttgc cgcggcggat gacagagagt ttcacgaccg cgcccggctc
     5881 accgcttagc agcagctgcg cctgctcgac ggccatctgg ccggtggtga agccagcgat
     5941 cttttccagc acgtcgccca atcctaaacc ggctttcttg gcgggactat ccggcaaagc
     6001 ggaaatgaca ctgatatacc cgaaccgctt agtgagagcc aaaccggcgc cggccttgga
     6061 gttggccgcg ctcttttctt cgaagtcttt gtattcgagc ggactgagat aactggattg
     6121 agaatcgagg gagtcaagca gtccgtggag agcgccgctc gtgacctggt gcatgttcgg
     6181 gtcgtcgacg taatcgcgct gaatgtgttc gaggacttca ctataaacgg tgagcgcgcg
     6241 gaaggctttg tcatccgaag agcggcccat cacgtagccc gacgtaacgt agaggaacac
     6301 gacggctgac agcgcaatca cgattttctt ggtgagagaa ctcattccct tttccttttc
     6361 gcccttgcgc accctcagtt ggcgcgccga actgcagctc cgcagagtcc gctgcgaaca
     6421 gcatcgcatc cgagattgga atgcgcccca tggactccgg gacacccagt atagcactgg
     6481 gttcacgagg gcggctacct ggctacttat gcgcgaacgt ttggcggccg ctgggaacgc
     6541 tgtaggaatc ggcatcagcg agcggcatgg cagcggtgat gtatgtttgc gagatacggt
     6601 cgtgccaagt gaagtggtcc tcgtcccata gtgcccagaa gaagccgatc atcaacgtgg
     6661 cgccagaaag caggtatccg aagacgcgcc agagaagctg ccgggtatcc ggaagactgc
     6721 catcgaggcg aacgatggaa aggccgcgca actgcattcc gggagtcgat ccggcgagaa
     6781 tgacaaaaag cgaaaggtag agggcgtaaa agagataggc gatcgccaca taggcgatgg
     6841 cgtcgctttt gccgagagta agttcacccc ccgccgaccg gctggcaaac aggaacagtc
     6901 caacgaatcc ggcacaggta agcgccagga agcagaagtc gatgtagcat gccgaacgtc
     6961 gatgggccaa tggggcgacc ggtacgagag cggtttgtgg gcgtgcacga tcgtacggag
     7021 aactcgaaaa atccagttcc ggctggacgc ggatttcgac gcgttcggtc tgtgggggct
     7081 tttgccggag gcgaggcgca ggccgctgtg gcgggcgctc acgaacttcg tcatcgagat
     7141 ccagagtggc gtgcgtgggc gcgggtttct taaagggaag gcccggttgg gtatcgggaa
     7201 gatcggggcg caaccggccg cgacgcgtgc gatattcttc caggcgtcgc gatacttcag
     7261 tgcgccattc aggctcagcg ctttcggcga gatgaaccgc gccgactgcc acggattcgt
     7321 agacttcttc gttttcagat ggggagacgt cgcagaaaga gcagctctct gtgtcgttga
     7381 gcaagagagc gccacacttc gagcatatgt gaatcactgg tccgttttgc gctgcggaaa
     7441 ttcccagtgg aacttgcgaa tcatatcacg cggatgaaag aggcggcgga gaaaaaccgt
     7501 cgcccggcgt gtccgattgc gcaacacggc attgcgtccc tatcaggaaa ttgcccgctt
     7561 cagaaaatcg tcaatgcgcg cattggccgg cgtccccagc gggaggggga aaatattgaa
     7621 cgcgtgcggg gcgccgggat atacgtccaa ctccgcttga tttccggcgg cgatccagcg
     7681 ggcgtacagg aaaagcgtat cgtccagcag cggatcgatc gttccgacgg tgaaaatcgc
     7741 cgggggcata tttttcaggt cggcgtagag cggcgagaca tccggatccc tcggattgac
     7801 gccagccgga aggtaagcct cgcggaactt atgtatgctg atctttccga cgaggattcc
     7861 cctgtcggga gtgagcgttt ggctgggagt catcgaggaa tcgaaattgc cgtaggagag
     7921 aactgcggcg cggaagccag tgtagccgtg gcggtcgcgc atacggagca gagtcgccgc
     7981 tgtgagagtg gcaccggccg attcaccgcc gattgacagc acgtccgcgc cgaactccga
     8041 tttggcgttc ttggtcagcc agagggcgac agattcgcag tcgtcccacg cggcgggata
     8101 aggattctcg ggcgcaaggc ggtactcgac gctgacgcaa gccatgccgg tgctttgaac
     8161 gatgcgttcg agcataggat cctggccttc ggaggagccg aagaccaacc ctccgccatg
     8221 aatgtggata tagacgccct tcgcacgttc gggggcgatc acgcgaaccg gaactttgtg
     8281 gccgcccttg ccatcaattt ggatagtgcg cgcgcgggcg gacttttcgg gcggcggaaa
     8341 cgggcctctg ccggcagcgg cagcttcgcg aaaggccggc gcgccgatgt cccaccagtc
     8401 gggaccgccc gacagcgctt tccggaacgc ctcattggcc accagagttt ccgcggagac
     8461 ggcttgtggc ttaaatacag agggatcgaa aagaccgctc ttggcagggc ttgcttcgct
     8521 cattttattt gcctccgaat cgtcgtttct tcgccgcgga gtatatcacc cggtgaattt
     8581 cgttcaaccg ccgcgcccgc gtttgtggga acgcgcctgc gaagcgatta gtgctgcttg
     8641 ggatgagtag cgatcaagcc ttggtcatac attgcggcgt aatccaaata ttggctcacg
     8701 ggatcaggcg ggtaactgat ctcaactttc tcgatagcgc ccgtctttcc gaag
//
LOCUS       DATNDT010000031         2650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2811983, whole genome shotgun sequence.
ACCESSION   DATNDT010000031 DATNDT010000000
VERSION     DATNDT010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811983"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VN788_01120"
     CDS             complement(<1..890)
                     /locus_tag="VN788_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019535852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU19162.1"
                     /translation="MTAGPLIHVRELSKHFRAFHRREGILGSIQNLFVREYRTVAAVD
                     RVSFSIDRGEMVGYIGPNGAGKSTTIKMLTGILVPSSGEIVVNGFVPFRQRTQYVKTI
                     GVVFGQRTQLWWDIAVIESFKLLRRIYDVSQNDFDARMERFNEILGIRDYLHTPVRKL
                     SLGERMRCDLAAALLHNPPLLFLDEPTIGLDVVAKDHIRHFLRAINREFHTTVLLTTH
                     DLDDIEELCRRIMIIDHGRVLYDGPLGQLKERLLRTKQIKFALKDRAQAAAIAAFSRD
                     GLELDKVDELTFRISFDRTQV"
     gene            complement(941..>2650)
                     /locus_tag="VN788_01125"
     CDS             complement(941..>2650)
                     /locus_tag="VN788_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU19163.1"
                     /translation="YALDAKTGKEIWSFDPHADLRVNREACCDEVNRGVAVWKGKVYV
                     ASFDGKLFALDAATGRVVWQADTIIDHKKGYTSTGAPEVAGKVVVIGNGGGEYDARGY
                     ISAYNLDTGKLKWRFFTVPGDPSKPYENPELKAAAKTWDPKSNWNMGGGGTVWGTMDY
                     DPELNLLYFGTGNGTFYDRSKRSPSGGDNLYIASVIAVNPDNGRMAWYYQEVPGDQWD
                     YDVVQPMILANLKIDGKMRKVLMHASKPGFFYILDRKTGKLLSADKYVPVTWASHVDM
                     KTGRPVEAPNARDFRYSTDGKSLISPSPMGGHNWNPMSYDPKTGLVYIPEIENGQTTI
                     FSGKTFLRAWDPIRSKVVWEVPNSDWWDHAGVLATAGGLVFQGTGTGYFCAYDADTGQ
                     KLKSIEVGTTIIAAPMSYTIDSVQYVAVMAAWGGGGWNFPHPTSAADQRGNEGRIIAF
                     KLGGGPVPIPALLPPIKPIPQPPPLTASAETVEQGGKLFAARCSSCHANAPHTLTPDL
                     RRMSPETHDAFQKIVLGGLLENAGMPRWDGILSAADVDAIHAYLISISWDAYKKQQAA
                     RNN"
BASE COUNT          523 a          734 c          796 g          597 t
ORIGIN      
        1 acttgagtgc gatcgaagct gatgcgaaag gtcagctcat ctaccttgtc cagttcgagg
       61 ccgtcgcgcg aaaaggcggc gatggcagcg gcttgcgcgc gatctttcaa tgcgaacttg
      121 atctgtttcg tgcgcagcag cctctctttt agctggccga gtgggccatc gtaaaggacg
      181 cgtccgtggt cgatgatcat gattcggcgg cagagttcct cgatatcgtc gagatcgtgc
      241 gtggtgagca aaaccgtggt gtgaaactcc cgattgatgg cgcgcaggaa atggcggatg
      301 tgatccttcg cgacgacatc gaggccgatg gtcggctcat cgagaaagag cagcggggga
      361 ttgtgcagta gagctgcggc caggtcgcag cgcatgcgtt cgccgagtga aagcttgcga
      421 accggagtgt gcaggtaatc gcgaatgccg agtatttcgt tgaagcgctc catacgcgcg
      481 tcgaaatcgt tttgagatac gtcgtaaatg cgacgcagca gcttgaacga ttcgatcacg
      541 gcaatgtccc accaaagttg ggtgcgctgg ccgaaaacca cgccgatcgt tttcacatac
      601 tgcgtgcgct ggcggaacgg aacgaatcca ttgacgacaa tttctccgct gctgggaacc
      661 agaatgccgg tgagcatttt gatggttgtg gatttgccgg cgccattcgg tccgatgtag
      721 cccaccattt cgccgcgatc aatcgagaag ctgacgcggt ccacagccgc cacggtgcga
      781 tactcgcgta caaaaagatt ctgaatcgaa cccaggattc cttcgcgtcg atggaaggcg
      841 cgaaagtgtt tggaaagttc gcggacatgg atgagcggac cggcggtcat aaccgcaaga
      901 attctaacat ctgccgaagg gcactgcaga atcgccgcag ctagttattg cgcgcggctt
      961 gctgcttctt atacgcgtcc catgagatgg agatgagata ggcgtggatg gcgtccacgt
     1021 cggcggcgct tagaatgccg tcccagcgtg gcattccggc gttttccaat agcccgccga
     1081 gaacgatctt ttgaaatgcg tcgtgcgttt ccgggctcat cctgcgcaaa tcgggtgtga
     1141 gcgtgtgcgg cgcattcgca tggcacgaac tgcagcgcgc ggcgaagagc ttgccgccct
     1201 gttcaaccgt ttccgcggag gccgtaagcg gagggggctg cggaatgggc ttgatggggg
     1261 gcagaagcgc gggtatcggc acagggccgc cgccgagttt gaaggcgatg atgcggcctt
     1321 catttccgcg ctggtcggcg gcgctcgtgg ggtgcgggaa attccagccg cctccccccc
     1381 acgccgccat caccgcgacg tattgcacgc tatcgatcgt gtaagacatc ggagcggcaa
     1441 taatagtggt accgacttct atgctcttca gcttctgtcc ggtatctgca tcgtaggcgc
     1501 aaaaatatcc cgtgcccgtg ccctggaaga ccaggccacc tgccgttgcc agcactccgg
     1561 cgtggtccca ccagtccgag ttgggaactt cccagacgac tttgctgcga atcggatccc
     1621 acgcccgcaa gaaagtcttg ccggaaaaaa tcgtcgtctg gccattctca atttccggga
     1681 tgtagacgag gcctgttttt ggatcgtacg acattgggtt ccaattgtga ccgcccatcg
     1741 gcgacggcga aatcaggctc ttcccgtccg tcgaatatct gaaatcgcgc gcattcggcg
     1801 cttcgactgg gcgccctgtt ttcatatcta cgtggctggc ccaggtgacg gggacgtatt
     1861 tatcggcgga gagaagtttg ccggtcttgc gatcgaggat gtagaagaag ccgggcttcg
     1921 acgcatgcat cagaaccttg cgcattttgc cgtcgatctt cagattggcg aggatcatcg
     1981 gctgcaccac gtcgtaatcc cattggtcgc cgggcacctc ctggtaatac caggccatgc
     2041 ggccgttgtc tggattcacg gcgatgacgg atgcgatgta aagattgtcg ccgccgctgg
     2101 ggctgcgttt ggaccgatcg taaaaggttc cattgcctgt gccgaaatag agaagattca
     2161 gttccggatc gtaatccatc gtgccccaaa cggttccgcc gccgcccatg ttccaattgc
     2221 ttttggggtc ccatgtcttt gctgcagctt tgagctctgg attttcgtag ggtttcgatg
     2281 gatctcccgg cacggtgaag aatcgccatt tgagcttgcc ggtatcgaga ttgtaagcgc
     2341 tgatgtagcc ccgcgcgtcg tattcgccgc cgccatttcc gatgaccacc acttttccgg
     2401 caacttccgg cgcgccggtg gaggtgtaac cttttttgtg gtcgatgatc gtgtcggctt
     2461 gccaaaccac tctgccggtg gctgcgtcca gcgcaaaaag tttcccatcg aacgaagcga
     2521 cgtagacctt gcccttccag acagcgacgc cgcgattcac ttcatcgcag cacgcttcgc
     2581 gattcacgcg caagtccgcg tgtggatcga aactccagat ttccttgccg gtctttgcat
     2641 cgagcgcata
//
LOCUS       DATNDT010000032        11639 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2810470, whole genome shotgun sequence.
ACCESSION   DATNDT010000032 DATNDT010000000
VERSION     DATNDT010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11639)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11639)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11639
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2810470"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VN788_01130"
     CDS             <1..241
                     /locus_tag="VN788_01130"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU19164.1"
                     /translation="TFSPLLYTYTAEAFPTEIRNSGMGLAYGTGRLVNVVGPLIVVFL
                     FNHYGYTSVFVYMALCWVLLALTIGILGVRSRTLA"
     gene            complement(567..1976)
                     /gene="tcuA"
                     /locus_tag="VN788_01135"
     CDS             complement(567..1976)
                     /gene="tcuA"
                     /locus_tag="VN788_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent tricarballylate dehydrogenase
                     TcuA"
                     /protein_id="HXU19165.1"
                     /translation="MVHSSDQYDVIVIGGGNAAINAALAVRHEGKRVLLLERGSENMR
                     GGNTRHTRNIRYAHDAADDCCSGPYPEEVYLEDLVRQGAPVNMDLAKYTIHESKRLPQ
                     WMGEHGITWQPQLSGTLHQGHTNRWFLGGGKAMLNTYYRAAQRMGVETRYNAHVEDLI
                     IENDRFEAAVVKSGQNGNPPDLVRGRSVIVAAGGFEANLQWLKQYWGEAADNFIVRGT
                     PFNDGAMLKALLQKGVKSVGDPKEFHAITVDARSPKYDGGIAIRLDAVPFGIVVNKEG
                     RRFHDEGLNVWPIAYARWGGLIAEQPDQIAYAIVDSKTINKFLPPMYPPYKAGSFDEL
                     ADIANVNPKNFVETMAIYNRGTAGNTGVRIQYLDGICTREVTPPKTNWALPIDVPPFY
                     AFPMRPGITFTYMSVAVDDRARVVDQRDRPFNNVYAAGEIMSGNILTKGYVGGIGLVI
                     GAVFGQLAGREAANMRHTS"
     gene            2248..3165
                     /locus_tag="VN788_01140"
     CDS             2248..3165
                     /locus_tag="VN788_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXU19166.1"
                     /translation="MNNIFSIHSHSHTKRSSSDQQRLPFLRVAPGIEIAYRIDEFCDG
                     WAQADTVMMLHGIAETGDALKAWVPHFARHYRVVRPDLRGFGQSTPVGEHDPLSIAEL
                     ADDIAAMIDALQLGHVHLVGAKLGAQIGLELAQRRPAWLASMTLAGVLISPGKAIGHW
                     IEQWIGLVDQGGVVNWARATMPGRMGNSLSAEAMEWWINYMGSAPASAVKACFRMLPG
                     LTEPAHLEEVVCPTLVIVAVKPSHADQFNQQQPVAEVGRWLSRIPNSKMVELAADSYH
                     VAASHPDECADIAYAFIASQSSKNHRVTG"
     gene            3209..4543
                     /locus_tag="VN788_01145"
     CDS             3209..4543
                     /locus_tag="VN788_01145"
                     /inference="COORDINATES: protein motif:HMM:NF015905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmgE/PrpD family protein"
                     /protein_id="HXU19167.1"
                     /translation="MGSNSTVSQQLAEFIHATTFGDLPPATIEQAKSRVLDSLATAIA
                     SRSLPVPTVALQFVQKNRGEATIFGHEQRVPTVDAALVNGTLVNGTTHDDFLAKSHAG
                     AVVIPSGIAVAEEEGSSGREFLASVVLGYDLVARAYMGGPAMLPKFRASGVAGAVGAA
                     ATAGKLQKLTASELVNALGLSTMFASGFGEGFHAGTMDVKLNVGWACRSGVSASSLAR
                     LGATSAPSAFEGRSGFFNAFAGTAEKAGETLRDLGNPFLIEKVSYKERPVCIFVQTPV
                     HLAHRFAQETKPDPSKIKRVTIRVPDATYTNPGFKNVAPFSTPLQARVSARFCTAAAL
                     LGKPIDQYAFYANTTDPEVLALAEKVDLLDPVEDTEHVCLEIIYDGETYRLAGVEMET
                     LLPTMAKVVAKFKRLTMGFFGERMDQVLDTILNLDRVTQIRELTDQLRSQEA"
     gene            complement(4672..5634)
                     /locus_tag="VN788_01150"
     CDS             complement(4672..5634)
                     /locus_tag="VN788_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006700587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HXU19168.1"
                     /translation="MDRQVKVAVLDDYQGVALELADWSILEGRAEVTVFRDHLSDPDK
                     VGERLKPFDVACVMRERTPLPRSIIEKLTNLKLIVSTAGRNASIDMDAAKERGIPVCG
                     TPSTSHGTVELTWALILVVMRNLIAEVASVRNGKWQVTVGGDLQGKTIGIVGLGKIGA
                     RIASVARAFGMNIVAWSQNLPPEVAQEQGVRLVSKEQLFRESDIVTLHLVLSPRTKAI
                     VGAAELKLMKPSAYLVNTSRGPLVDEEALIEALKSHTIAGAALDVYDAEPLPESHPFR
                     SLGNVLATPHIGFVTQEGYKIFYRGIVEDIAAWLDGNPLRVMNY"
     gene            complement(5865..6785)
                     /locus_tag="VN788_01155"
     CDS             complement(5865..6785)
                     /locus_tag="VN788_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HXU19169.1"
                     /translation="MTEITFQRLAIIGFGEVGGIFGTELAAAGVHVTVFDILLNKGQS
                     REAMLTKAKAAKVRAADSLKEAVLGAELLIAAVTASSATQVAKDAAAVLKPGQFYMDL
                     NSVSPETKTKVAESIGKSGADFVEAAVVAPVAPQRLKVPMLLGGARAGELASRLQAMG
                     MDTTAVSERIGVASAIKMCRSVVMKGMAALAIESLFAARRYGVEDAVIASFEATYPSM
                     GWAKSLPDNLAKRAAEHSRRRAAEMREVAETLREVGINPVMALGTAEIQDWLTAEMEA
                     GKFVFNAKDFSWRSLIDAIDARTNPDRKRA"
     gene            7413..9101
                     /locus_tag="VN788_01160"
     CDS             7413..9101
                     /locus_tag="VN788_01160"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU19170.1"
                     /translation="MNRVVRPAAIIVIIAAAGLLAAQTRQFNPVTEEMLRKPSPNDWL
                     NWRRTDNAWGYSPLDQINRNNVKQLQLAWAFPMQGGWQEPTPLVHDGVMYLPNAGSVV
                     DALDASSGDMIWEYRRDLPKFSLPGHDTEHQLLDEPMRNLSMFEDKIFLGTADAHMVA
                     LGARAGKVVWDTQASDYKDGLDYRAGTVVVHGKVIGGLVGCGTYVESKCALIGHDAET
                     GRELWRVLSVAKPSEPGGDTWSDLPLLFRSGGDMWIAGSYDPTLNLVYWGTAQAKPWA
                     RVSRHTDGDALFTDSTIAVNPDTGKMLWYHQYLPGETEDMDDVFEKILVDSPEHKSLF
                     EMGKLGILWEIDRQTGKFIHATDLGYQNLVDVAPDGKVTYRPGKIPVAGQSIDFCPSY
                     GGIKNWQAMAYHPETHAFYIPTHPFTCETATYPVEKLERGGGGSGGVKQVMRPNPLGD
                     GSGGAFIAMNTSGKILWKHEQRASYSTSALTTAGGLVFVGDNRNVYAFDVKTGELLWQ
                     TKTMAPPTGFVITYSAGGRQFIAIPAGNRFIAGAPRLNTGNAILVYALPQTANR"
     gene            9214..10383
                     /locus_tag="VN788_01165"
     CDS             9214..10383
                     /locus_tag="VN788_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HXU19171.1"
                     /translation="MKQKSTDEGEWQTNNGRRDFVKGVTTAGLLGAVGIASTLTGQAD
                     ASSNVPQSPGGRSRSRGGVMDCRSPLAFQDSVPNSLKILSQYFQALSERKLDGMAELM
                     HFPFLTHEGPEAQLVHNRGELISSPPRSMNVTAKGRSKIAPGAYDILDFIQMHIYSPV
                     GAGLSLEYSRFAPNGTKIFSCHGLYGVTNNDGKWGIEWVSTIFKPANQVGRDQSYNYP
                     ILNTAIHESHRNHVMARKYDDLPELRQSVFDPFPHGSLSLGPSVVRDASGKPRQEYRI
                     EGVTSRLRYSNGDTQAEMDKQTFGQAQFSKTSGEDVGGWALSIEIPDTRMLYASAEKG
                     HFYSGYYRYTEDGSIISEHRYLGALVNWKGKWYGNDISRIIGQVVYQDRSNDIRS"
     gene            10465..>11639
                     /locus_tag="VN788_01170"
     CDS             10465..>11639
                     /locus_tag="VN788_01170"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU19172.1"
                     /translation="MNSPFRSAAPATALDAVYGRVTRKFLPLLFTCYLFNYVDRTNIG
                     FAQLQMRGDLGFSAMMYGLGAGIFFISYSLFAVPSNLLMAKIGARKTIFGCLTLWGLT
                     SSATMLVRTPSEFYVARFLLGVVEAGFFPGVIFYITQWYPSHRRANVTGIFQSATVVA
                     GVVSGLLSGALITYLDGLWKLRGWQWMFLLEGLPSLVMGILVLLYLDDRPQQAAWLSY
                     EEKALILEALDNDSATASGSQTFREALADWRVYLLGAIFFASVIGTYALAFWQPMMIR
                     SFNISSIMRIGIYSTIPALAAVAAKILIGYHSDKQRELRWHFAAPAFAGAIGMLLIPF
                     AAQNPLVGIVCLALATAGVHGCIPVFWSMPGLYLSGTAAAGGIALISTIGNLAGAVGP
                     "
BASE COUNT         2545 a         3206 c         3208 g         2680 t
ORIGIN      
        1 cacgttctcg ccgctgctct acacttacac ggcagaagcg ttcccgacgg agattcgcaa
       61 ttctggcatg ggtctggcgt acggcacggg ccgccttgtg aatgtggtcg gcccgttgat
      121 cgtcgttttc ttattcaatc attacggata cacgagcgta tttgtttata tggccctttg
      181 ctgggtgctg cttgcactta ccatcggcat tttgggtgtg cgaagccgca cactcgcgta
      241 gggcgcccgt tcttattgcc tggagcttga gaatgtgtcg atgcctcggc agccgaatga
      301 tgtcggcgcc gcgcgatgcc gcgttgaaag cagcgcgcta tataaaatct cggaggcgac
      361 gcctagattt ggtagccgta cggcttggcg ggattttcca ccaagatccg gtgaggctgc
      421 gtacaatccg gcgcccgacc agatagaaga tcgagaagtt ttccatcgtc cggcggtcgc
      481 gccttacaca ttcccggatg cagatcagtg actcgctcag tgaagggcat cggcatcatg
      541 ggagcgccgc tacgggatga agaacatcac gatgtgtgcc gcatgttggc tgcttctctg
      601 ccagcgagtt ggccgaatac ggcaccgatg accaatccga taccgccgac gtaacctttg
      661 gtgagaatgt ttccactcat gatttcgccg gccgcataaa cattgttgaa aggcctgtct
      721 ctttggtcca ccacccgtgc cctgtcgtcc acggccacgc tcatatacgt aaaggtaatg
      781 cccggccgca tcggaaaggc gtagaaggga ggaacgtcaa tggggagggc ccagttagtt
      841 ttaggcggtg tgacttcgcg ggtgcagatt ccatccaagt attggatgcg aacacccgtg
      901 ttacccgcgg ttccacgatt gtagattgcc atcgtttcca caaagttctt gggatttacg
      961 ttggcgatat ctgcaagctc gtcaaatgag ccggccttgt aaggcgggta catcggtgga
     1021 agaaacttgt tgatcgtctt cgaatctacg atggcatagg ctatttggtc cggctgctcg
     1081 gcaatcaggc cgccccacct ggcgtaggca attggccaaa cgttaaggcc ctcatcgtga
     1141 aaacgccgtc cctccttatt gaccacgatg ccaaaaggaa cggcgtcaag tctgatggcg
     1201 atgcccccat cgtactttgg tgagcgagcg tccaccgtaa tagcgtggaa ttcctttgga
     1261 tctcctacgg actttacacc tttctgcagt aatgccttca gcatcgcgcc gtcattaaaa
     1321 ggtgtgcccc ggacgatgaa gttgtcagcc gcttctcccc aatactgctt gagccattgc
     1381 aggttggctt caaatccgcc cgcagccacg atcaccgagc gtccgcgcac cagatcagga
     1441 gggttcccgt tctggccgct cttgacgact gcagcttcga agcgatcgtt ttcgatgatc
     1501 aagtcttcca cgtgcgcatt atagcgggtc tccactccca tccgctgcgc cgcacggtag
     1561 taagtattta gcatcgcttt tcctccgccc aggaaccagc gattggtgtg cccctggtgg
     1621 agggtgccgg acagctgcgg ttgccaagtg atgccgtgct cacccatcca ttgtgggagt
     1681 ctcttcgatt cgtggatggt gtacttggcg aggtccatat tgaccggggc accttggcgg
     1741 acgagatcct cgaggtaaac ctcttcaggg tagggtccgg agcagcagtc atcggcagcg
     1801 tcatgtgcgt aacggatgtt tcgcgtatgc cgggtatttc ccccgcgcat attttctgaa
     1861 cctcgttcca aaagcagaac ccgtttcccc tcatggcgaa ccgctagagc agcgttgatc
     1921 gcggcattgc cgcctccgat gacgatgacg tcgtattgat cagatgaatg aaccaaaaaa
     1981 cccctccacg aatgccccac gatcgtgggc gtcagctctt gctcttatgg tgaagcgctc
     2041 tataataaca ccccgtcagc attgtcgagc cacaaaccgg gtccggagaa atttgccact
     2101 cagcaatgag tcctgcaggc agcagccacc ccgcacatcc tcactgacgc tgttattcaa
     2161 tcgatggcaa ccattgttcg agggggcagt acaaggtacg atttacgagc cataggaaat
     2221 tctggctgaa gtagaaagga gcacttcttg aataatatct tctcgatcca ctcgcattcc
     2281 catacaaagc gatctagttc cgatcagcaa cgattaccgt ttctgcgcgt tgcgcctgga
     2341 atcgagattg cgtatcggat cgacgagttt tgcgacggat gggcgcaagc cgacacggtg
     2401 atgatgttgc acggtattgc tgaaacgggg gatgccttga aggcctgggt tccgcacttc
     2461 gcgcggcact atcgggtcgt tcggcccgac ctccgagggt ttggccagtc aacgccagtg
     2521 ggcgagcacg atccgctttc gattgcggag ttggccgatg acattgcggc gatgattgat
     2581 gcgttgcagc tcggccatgt gcatctggtc ggagccaagt tgggcgcgca aatcgggttg
     2641 gagctggctc agcgacggcc ggcttggctg gcatccatga cgcttgctgg cgtgctcatt
     2701 tcgcccggga aggcgatcgg gcattggatc gaacagtgga taggtttagt cgatcagggc
     2761 ggtgttgtga attgggcacg cgctacgatg cctggtagga tgggtaactc gctcagcgct
     2821 gaggctatgg aatggtggat taactacatg gggagcgctc cggcgtcggc agtaaaagct
     2881 tgctttcgca tgctgccggg cctgacggaa cctgcccatc tggaagaagt cgtgtgtccg
     2941 acgttggtaa tcgtcgccgt caaaccgagc catgcggacc aattcaacca gcagcagccg
     3001 gtggccgaag ttgggcgctg gctctcgcga attccgaatt caaagatggt tgagctagcc
     3061 gccgattcgt atcatgtggc agcctctcac ccagacgaat gtgccgacat cgcatacgcg
     3121 ttcattgcaa gccaaagctc gaaaaatcat cgggttaccg gataagggct ctaaggatcc
     3181 acctaccgac atcaacagga gaactttcat gggtagcaac tccacggtaa gtcagcaatt
     3241 ggccgaattt attcacgcta ctacttttgg tgacctgcca cccgcgacta ttgagcaggc
     3301 aaagtcccgg gtgttggatt cgctggcgac agccattgct tcgcgcagct tgccggttcc
     3361 caccgtagcg ctgcagtttg tccaaaaaaa ccgcggtgaa gcaacgatct tcggccacga
     3421 acaacgagtg cccacagtcg atgccgcgct cgtcaatgga acgctggtca acggcactac
     3481 gcacgatgac ttcctggcta agtcgcatgc gggcgcggtt gtgattccat ctggcatcgc
     3541 cgtcgccgag gaggaaggga gctcagggcg ggagtttctc gcgagtgttg ttctaggcta
     3601 cgaccttgtg gcgcgggctt acatgggggg gccggcgatg ctgcctaaat ttcgggcttc
     3661 cggtgttgct ggagccgtgg gcgccgcagc aaccgcggga aagctgcaaa aactcaccgc
     3721 ttccgaattg gtgaatgccc ttggactttc gaccatgttt gcgtccggat tcggcgaagg
     3781 cttccatgct ggcacaatgg acgtgaaact caacgtcggt tgggcctgtc ggagcggtgt
     3841 atctgcatcc agcctggctc ggctcggagc gacgtccgcg ccaagcgcgt tcgagggtag
     3901 gtcaggcttc ttcaatgcct ttgccggtac ggctgagaaa gccggtgaga cattgcgcga
     3961 ccttgggaac cctttcctca ttgaaaaggt cagttacaaa gagcgtcctg tctgcatctt
     4021 tgtgcaaact cccgtgcatc tggcccacag atttgcgcag gagaccaagc cggatccgag
     4081 caagatcaaa cgggtgacaa ttcgggtgcc ggacgcaaca tacacgaatc caggattcaa
     4141 gaatgttgca cccttcagca ctccgctgca ggcgcgggta agcgccagat tctgcacggc
     4201 ggcggctttg ttagggaagc ctatcgacca atatgcgttt tatgccaaca ccacagaccc
     4261 ggaagtcttg gcgcttgccg agaaggtcga tctgctcgat ccagttgaag ataccgaaca
     4321 tgtctgtctc gaaataatct acgatggcga gacgtatcgc ctggcgggtg tcgagatgga
     4381 aacgctactg cccacaatgg ccaaagttgt cgcaaagttt aagcgcctca cgatgggctt
     4441 ttttggcgaa cgaatggatc aggtcctcga tacgattctc aacctggatc gcgtgacgca
     4501 aatccgcgaa ctgaccgatc agctccgaag ccaagaagcg taaaaaccct ttttaggtag
     4561 ttcaacacat agaagaagag cgagaataaa tctcagcgcg attttccgat agcagaggct
     4621 tgccggaact gcgtgaactt agtttcaaac gtttcggagg caacttataa gtcaataatt
     4681 catcacacgt aacggattgc catcgagcca ggcggcgata tcttcgacta tgcctcgata
     4741 gaagattttg tacccctctt gggtaacaaa gccgatgtgc ggcgttgcga ggacattccc
     4801 gagagagcgg aatggatgag attcaggtaa cggttcggca tcgtaaacgt cgagagccgc
     4861 gcccgcgatg gtatggctct taagtgcttc gatcaatgcc tcttcatcga ccaaggggcc
     4921 acgtgacgta ttcacgagat aagcactcgg cttcatcaac ttgagttcgg ctgcgccaac
     4981 tatggctttc gtgcggggac tcaaaactag atgaagagtc acaatatcgg actcccggaa
     5041 caattgctcc ttgctaacca gccgaacacc ctgttcctgg gctacttcgg ggggaaggtt
     5101 ctgactccat gcaacgatgt tcataccgaa tgcacgtgca acgctcgcaa tgcgtgcacc
     5161 gattttccca aggcctacga ttccgatcgt cttcccctgc aggtcgcctc caaccgtgac
     5221 ctgccacttc ccgttccgta ccgaagcaac ttctgcgatc aagttgcgca tgacgactaa
     5281 aatcaatgcc catgtcaact cgacggtacc gtgcgaggta gatggagttc cgcagactgg
     5341 gatgccacgt tctttagccg catccatatc gatcgaagca ttacgccctg cagtcgatac
     5401 aataagcttc aagtttgtca atttctcgat gatcgaacgg ggaagtggtg ttcgttcgcg
     5461 catcacgcat gcgacgtcga atggcttcag acgctccccc actttatcgg gatcagataa
     5521 gtggtctcga aacaccgtga cctcggccct accttcaaga atcgaccagt ccgccaattc
     5581 taaggcgaca ccttgataat cgtcgagaac ggcaacttta acctgtctgt ccatggtaat
     5641 aacgagttca cccgggaaat gccatcaaag ttgaatcatc attgtatatg ccaccgcatc
     5701 ctgatatccg tgaccatttc ccatagatat tttgcatgcg gctcgcgtac tgcggcaatc
     5761 ccctcagtat cgagaatcga ttgaattgcg gtgaagtatt tcgtacgcct ttccactccg
     5821 atccataagc aagaagattt gcaaaccgcg cgaattcaaa agatctaagc ccgcttgcgg
     5881 tccggatttg tcctcgcatc aatcgcgtcg atcagcgacc gccaggagaa gtccttcgca
     5941 ttaaacacga attttccggc ttccatttct gccgtgagcc aatcctgtat ttcagccgta
     6001 cccaaggcca taaccggatt tattcccact tctctcaggg tctccgcaac ctcccgcatt
     6061 tcggcggcgc gtcgacggct gtgctcagcg gctctcttgg cgaggttgtc cggaaggctt
     6121 ttagcccacc ccatgctcgg ataagtggct tcgaaagacg caatgacggc atcctcgact
     6181 ccatagcgac gcgcggcaaa aagggattcg atcgcgagcg cagccattcc cttcatgaca
     6241 acgcttcggc acatcttgat cgcggaagca acgccaatgc gctcgctgac ggccgtcgta
     6301 tccatgccca ttgcctgaag tcggcttgcg agctcgcccg cgcgagcgcc gcccagcagc
     6361 atcggaactt tcaggcgctg cggcgcaacc ggcgcgacga cggcagcttc cacaaaatcc
     6421 gctcccgatt tcccgatcga ttcggctact tttgttttcg tctccggcga tacggagttc
     6481 aggtccatgt agaactgacc cggcttaaga acggctgccg catctttcgc cacctgcgtt
     6541 gctgagctcg cggtaacggc cgcgattaaa agttccgcgc ccagcacggc ctccttcaag
     6601 ctgtccgccg cgcgaacttt cgccgctttg gcctttgtaa gcatcgcttc cctggactgt
     6661 cccttattga gaagaatgtc gaagacggtt acgtggacgc ccgcagcggc caattccgtc
     6721 ccgaagatgc cacccacctc gccgaagccg ataattgcaa ggcgctgaaa tgttatttca
     6781 gtcatgagag ttcattttcc ctttctgtga atgccgcaga atgtttgcta aattcttctc
     6841 gcggcggatg acaccttcat gcgttgcgcg ctattcccca aaaaacagtt cgccgacgtc
     6901 tggctcatcg ggcagagtcc aaggctctgg aaatgatgcg acggacttcc tcgaccgcgt
     6961 cgcccggagt atgctgctac cgctgcgcct tcgcagcgag ttcgtcgccg gtcagcgtgt
     7021 tttgtggatt gcttctagcg gcagtcacgc cagctttcaa gtttgcgccc atcgcgggtt
     7081 tcagcttcca cccatcccga acgggcacta aagattgtat gccaatgtcg cttaatcgcc
     7141 ataggtgaaa agtcattcag tagggacaag tgtcagcggc catgtaggtc ggaagcgaga
     7201 aacaggagcc aatcagggga acgcctagtc gtgccgcctc gccaccaacc gccgtcagag
     7261 cgatctcgga ttgacaccag aggacgaccg gcctatggtc tttgcgaata aatcaaattg
     7321 agatcagaaa ttccggatag atgtggcatt cgggtcaaga ccgaaatccc gcacttcgtt
     7381 ggccgggcct tcatagggtt ccaggagaaa gtatgaatcg cgtagtccga cctgcagcaa
     7441 tcatagtgat cattgcagcg gcgggcctgc tagcggccca gacgcgccag tttaatccgg
     7501 tcacggaaga gatgctgcgc aagcccagtc caaacgattg gttgaactgg cgccgcaccg
     7561 acaatgcatg gggctatagc cctctcgatc aaatcaatcg caataatgtc aaacagcttc
     7621 aactcgcctg ggcttttccg atgcaaggtg gctggcagga gccaactccc ctcgtgcatg
     7681 acggcgtaat gtatttgcca aacgcaggca gcgtggttga cgcactcgat gcatcgtcgg
     7741 gtgacatgat atgggaatat cgccgggatc ttccgaagtt cagtttgcca ggtcatgata
     7801 ccgagcatca attgctcgat gagcctatgc gcaaccttag catgtttgaa gacaagattt
     7861 tccttggcac ggctgatgcg catatggtcg cgctcggtgc ccgtgcgggt aaggttgtgt
     7921 gggatacgca ggcttccgac tacaaagacg ggcttgatta tagggctggc acggtggtcg
     7981 tacacggcaa ggtgatcggc gggctcgtgg gctgtggaac ttacgttgag agcaaatgcg
     8041 ccctgatagg tcacgacgcc gagacaggca gagagctctg gcgcgtgttg tctgtcgcaa
     8101 agcccagtga acccggcggc gatacgtgga gtgacttgcc gttgcttttc cgctctggtg
     8161 gcgacatgtg gatcgctggc agctacgacc cgactctaaa cctcgtttac tggggcactg
     8221 ctcaagccaa gccctgggcg cgagtctcac gtcatacgga cggcgacgca ttattcaccg
     8281 attcaacaat cgccgtgaat ccggataccg ggaaaatgtt gtggtaccac cagtatcttc
     8341 ccggagagac tgaagatatg gacgacgtct tcgaaaaaat tctggtggat tctcccgagc
     8401 acaaatcgtt atttgaaatg ggaaaactgg gaattctttg ggagattgac cggcagaccg
     8461 gcaaattcat tcatgccacc gatctcggtt atcagaatct tgtcgacgta gctcccgacg
     8521 gaaaagtcac ttatcgcccg gggaaaatcc cggtggcggg tcagtcgatc gatttttgcc
     8581 cgagctacgg gggcatcaaa aattggcagg ctatggcgta ccatcccgaa actcacgcgt
     8641 tctacattcc cacgcaccct ttcacgtgtg aaacggcaac ctatcccgtc gaaaaattgg
     8701 aaagaggagg cggagggtca ggcggagtga agcaagttat gaggccgaat ccgctgggcg
     8761 acggaagcgg cggcgccttc atcgcgatga acacctctgg gaaaattctc tggaaacacg
     8821 agcagcgcgc aagttattcg acttccgcgc tcaccaccgc cggcggcctc gttttcgtag
     8881 gagacaatcg caatgtttat gcgtttgacg tgaaaacggg cgagttgctc tggcaaacga
     8941 agaccatggc gcctcctacc ggtttcgtga tcacgtattc cgcaggaggc cggcaattca
     9001 tagccattcc cgcggggaac cgtttcatcg ctggggcgcc acgtctcaac acaggaaacg
     9061 cgatcttggt ttacgcgctg ccgcaaaccg caaatcgatg agagatagag agtttctgat
     9121 atcattgcgg tgctgaatcg tgtgctacgt cttcatcgcg agttacatca atcagcctgt
     9181 ttgatatcag ccagcgtgtt gggaggcttt tttatgaaac agaagagcac tgacgaaggc
     9241 gaatggcaaa cgaacaatgg acgtcgcgat tttgtgaaag gcgtgacgac agccggcttg
     9301 ttgggagctg tcggaattgc atcgacactg acggggcagg cggacgcctc ctcgaacgtt
     9361 ccacaatctc ccgggggcag gagccggtct cgaggtgggg ttatggattg taggtctcct
     9421 ctagcgtttc aagattctgt tccaaattcg ctgaaaattc tctctcagta ctttcaagcc
     9481 ctgagcgaac gaaaactcga cgggatggct gaactcatgc acttcccatt tctcacgcac
     9541 gagggaccgg aggcgcagct tgtccacaat cggggggaac tgatttcgag tccgccccgt
     9601 tctatgaacg taaccgccaa aggacgcagc aaaatagccc ccggtgcata cgacatactg
     9661 gattttatcc agatgcacat ctacagtccc gtaggagccg gactttccct cgaatacagc
     9721 cgctttgcac ccaatggcac aaagatcttc tcgtgtcatg ggctgtacgg tgtaactaac
     9781 aacgacggaa aatggggtat cgagtgggtc tcgacgattt ttaagccagc gaatcaagtc
     9841 ggccgagacc aatcgtacaa ctatccgatc ttgaacacgg cgatacatga gagtcaccgg
     9901 aaccacgtga tggcccgcaa atacgatgat ctgccggagt tgcgccaaag tgttttcgac
     9961 ccttttccgc atggctcgct ctcgctaggc ccttctgtcg tccgtgatgc tagcggcaaa
    10021 ccgcgacagg agtatcgaat cgagggtgtg acgtccaggc ttcgctattc caatggagac
    10081 acccaggcag agatggacaa gcaaacattc ggccaggctc agttctcgaa aacttctgga
    10141 gaagacgtcg gcggctgggc actctccatt gagatcccag acactcgcat gttgtacgcg
    10201 tctgcggaaa agggccattt ctattccggt tactatcgct acaccgaaga tggctcgatt
    10261 atttctgagc atcgctatct aggcgctctc gtaaactgga aagggaaatg gtacggaaac
    10321 gacatatcga gaatcatcgg acaggttgtt tatcaggacc gctcgaatga catccgttcc
    10381 tgacgggcag atgatactgg tggtccgact ctttgttaac gggacgcaag ctcgccttcg
    10441 accgggtcgc ttactcgact ggcgatgaac tcacctttta gatctgccgc tccggcgacg
    10501 gctctagacg ccgtttacgg cagagtgacc cgaaagttcc tcccgttgct cttcacgtgc
    10561 taccttttca attacgtcga tcgcaccaat ataggctttg ctcaattgca gatgagaggg
    10621 gacctcggct tcagtgccat gatgtacggc cttggcgcgg ggatcttttt catcagctac
    10681 tccctgtttg cagttccgag caacctgttg atggccaaga ttggtgcccg caagacgatt
    10741 ttcggctgcc tcaccctttg ggggctgact tcttcggcga ccatgctggt ccgcacaccc
    10801 tccgagttct atgtggctcg attcttgctt ggagtcgtgg aggccggatt ctttccggga
    10861 gtgatcttct acatcactca atggtatccg tctcaccgcc gggctaacgt tacgggcatt
    10921 tttcaatcgg ctaccgttgt ggccggagtc gtttctgggc ttctttcggg agcgctcatc
    10981 acgtatttgg acggactctg gaagttacgc ggctggcaat ggatgttctt gctcgagggc
    11041 ttgccgagcc tcgtgatggg gatactggtt ttgctctatc tggacgatcg tccgcaacaa
    11101 gcagcatggc tttcctacga agaaaaggcg ctcattctcg aggctttgga caacgattcg
    11161 gcgacagcct ccggaagcca aacgtttcgt gaggcactgg cggactggag agtctacctg
    11221 ctgggcgcga tcttctttgc ttcggtgatt ggtacgtacg cactggcatt ttggcagccc
    11281 atgatgatcc gaagtttcaa tatttcgagc atcatgcgaa tcggcatcta ctctacaatt
    11341 ccagcgcttg ctgctgtcgc tgcaaagatc ttgatcggtt atcactccga caagcaaaga
    11401 gaacttcgat ggcattttgc cgctcccgca tttgctggag caattggaat gctgctgata
    11461 cccttcgccg cacaaaatcc attggtcggc attgtttgcc tcgcgcttgc cacagcgggc
    11521 gtgcacggct gcattccagt gttttggtcg atgccgggcc tctatctctc gggaacagct
    11581 gccgccggcg ggatcgcact gatcagcacc atcggaaatc tcgcaggagc cgtcggtcc
//
LOCUS       DATNDT010000033         3564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2493415, whole genome shotgun sequence.
ACCESSION   DATNDT010000033 DATNDT010000000
VERSION     DATNDT010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2493415"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..1162)
                     /gene="prfB"
                     /locus_tag="VN788_01175"
     CDS             complement(join(22..1086,1088..1162))
                     /gene="prfB"
                     /locus_tag="VN788_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_148264119.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HXU19173.1"
                     /translation="MAEHIEDLEQRYAELQKRIGLVRSYFDADSARKRIADLEVKINA
                     PDFWEDQEKAQALLQQRKQLEERAEGESALERKASDLDTYFHLAHEESNSEQRESLLE
                     DIAKEIAAADIYVSGLETKTLLAGESDAMNALVTIKPGAGGTESQDWAEMLLRMYLRW
                     AESKGMKAAVIDSSPGEEAGIKSATVEINGENAYGMLAGESGVHRLVRISPFDQQARR
                     HTSFASVFVIPEIDDRIEIVVKPEDLRIDTFRAGGHGGQNVNKVESAVRFTHLPSGIV
                     VQCQNERSQHKNRELAMKVLRSRLYELELEKRRGDMRKLDATKSDISFGSQIRSYVMQ
                     PYQMIKDHRTKLERGDIQKVLDGDIDPFIRAYLLYRRDVEQVAPK"
     gene            complement(1244..2773)
                     /gene="lnt"
                     /locus_tag="VN788_01180"
     CDS             complement(1244..2773)
                     /gene="lnt"
                     /locus_tag="VN788_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HXU19174.1"
                     /translation="MNWPWHTRVLLALASGLALALSFPNFNFSLLAWISIALLVLASY
                     QARPALAPLYGFLHALVFYPLCLTWIDVVVRQYGDVPPLTAVGLLALIAIAGGIILAA
                     FSLGIALASRRSALLACVLAPCLWVTLEFARAHLPIIGFPWNLAGYAVSSSLGLVQFA
                     SVTGIYGLSFLVAGYGALLGYAIISGRQRAWKSLISVTAGLILAGVGGGLLVPSAKSR
                     FVAHLVQTNFPQSYEYPPDWMPTHARDLDELEKISVDAARREPGLIVWPEVPAPFSLE
                     DPAFANRAQRIAHEAGTNFLVGVEDWKKDAAGKWIATNSAVLLNPAGQRTFTYDKIHL
                     VPFGEYVPLRNWLTFAGKLTADIGDFTPGTVYRVGNLPAGKFGVFICYEAIFPNEVRR
                     FTMNGAELLINMSNDGWFGRSSAPAQHLMMARVRAVENRRWLLRCTNNGFTVVVDPYG
                     RIVAKLPTDIRGELDAPYDFRGGLTPYARFGDWFAWLCLIGSVFLLGVALAPRATGRS
                     N"
     gene            complement(2788..3426)
                     /locus_tag="VN788_01185"
     CDS             complement(2788..3426)
                     /locus_tag="VN788_01185"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXU19175.1"
                     /translation="MGNGGKRGDRVLESRHLIVLFLGIVLLCGVFFTLGYVIGHNQYD
                     GAVHAAEAPEASALLANKAVKAAASNSAPSAAPANNEWDFYSKNASNRLEPAGKPSSP
                     AIPTSAPAPKDTSAPQAATESAKAIPVSSRFQPPRILKGSVVLQVAAVTHQSDALAMA
                     DALQRKKFSAFVLPPSGDNFYRVQVGPYRDQRTAESARNALERAGFKSIIKR"
     gene            complement(3430..>3564)
                     /locus_tag="VN788_01190"
     CDS             complement(3430..>3564)
                     /locus_tag="VN788_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HXU19176.1"
                     /translation="EPVSIQLRYLQTLTEIGVEKNTTVIFPLPIDMMSQWMKALGGNK
                     "
BASE COUNT          758 a         1059 c         1011 g          736 t
ORIGIN      
        1 cgaagcgcgc ccgagccagt gctatttcgg cgccacttgc tcaacgtccc gtcgataaag
       61 gagataagcg cgaatgaacg ggtcgatgtc accatcgagc accttttgaa tgtcgccgcg
      121 ctcgagtttc gtgcggtgat ctttgatcat ttggtagggc tgcatcacgt aagagcgaat
      181 ctggctgccg aaactgatgt cggacttggt ggcatcgagc ttgcgcatgt ctccgcgccg
      241 cttttcgagt tccagctcgt agagcctgga acgcaaaacc ttcatcgcca actcgcggtt
      301 tttgtgctgg cttcgctcgt tctggcattg gacgacgata ccacttggca agtgcgtgaa
      361 gcgcaccgcg gactcaactt tattgacgtt ttgcccgcca tggccgcccg cgcgaaatgt
      421 atcgatgcga aggtcttcgg gcttcacgac gatttctatg cgatcgtcaa tttcgggaat
      481 cacgaaaact gaagcgaacg acgtgtgcct gcgcgcctgc tggtcgaacg gcgaaatgcg
      541 caccaaccga tgcacgccac tttctcccgc cagcatcccg taggcatttt cgccgttgat
      601 ctccaccgtg gccgacttga tgcccgcttc ttcgccaggg gaagaatcga tcaccgcggc
      661 cttcatgcct ttcgattccg cccagcggag atacattcgc aggagcatct cggcccagtc
      721 ctgcgattcg gttccaccgg cgccgggctt gatggtgacg agcgcgttca tcgcgtcgct
      781 ttcgcccgcg agcagcgttt tagtttcgag gccggatacg tagatgtcgg ctgcggcgat
      841 ctctttggcg atatcttcga gaagcgattc gcgctgctca ctattcgact cttcgtgagc
      901 cagatggaaa taggtgtcga gatcgctggc cttgcgctcg agtgcgctct caccttcggc
      961 gcgctcttcg agctgcttac gctgctgcaa gagcgcctgg gccttttcct gatcctccca
     1021 gaaatcggga gcgttgattt ttacttcgag atcagcaatc cgtttgcggg cggaatcggc
     1081 gtcaaagata gctccgcacg agcccaatgc gtttctggag ctcggcgtag cgttgctcga
     1141 ggtcttcgat atgctcagcc atgttcaaat cctacacagg aaaatagaaa atcgaaactg
     1201 ctatgtgtgc ggaatgcggg atttcgacac cggcgtcgca accctaattg cttcgaccgg
     1261 tcgcccgcgg ggccagcgcc accccgagca ggaaaaccga tccgatcagg cacagccagg
     1321 cgaaccagtc gccgaatcgc gcgtagggcg tgaggccgcc gcgaaaatcg tagggagcat
     1381 cgagctcgcc gcgaatatca gtggggagct tggccacgat gcggccgtag ggatcgacca
     1441 caacagtgaa tccattattc gtgcagcgca gcagccagcg ccgattttca accgcccgca
     1501 cgcgcgccat catcaagtgc tgcgcgggcg cggacgaacg cccgaaccat ccatcgttcg
     1561 acatattgat cagcaattct gcgccgttca tcgtgaatcg ccgcacttcg ttgggaaaaa
     1621 tcgcctcgta gcagatgaac acgccgaact ttccggccgg cagattgccc acccgataga
     1681 cggtacccgg cgtgaaatcg ccgatgtcgg ccgtcagctt tccagcgaac gtgagccagt
     1741 ttcggagcgg aacgtattca ccgaatggga ccaggtgaat tttgtcgtag gtaaaggtgc
     1801 gctggcccgc cggattcaga agcacggcgc tattcgtcgc gatccatttg cctgccgcgt
     1861 ctttcttcca atcttccact ccaactaaaa aattggtgcc agcttcgtgc gcaatccgct
     1921 gggcgcggtt ggcaaaagcc gggtcctcca agctgaacgg agcgggaacc tcgggccaca
     1981 cgatcaaccc gggctccctc ctggccgcgt ccacactgat tttttccaat tcgtcaaggt
     2041 cccgcgcatg cgttggcatc caatccggcg gatactcgta cgattgcgga aaattcgtct
     2101 gaaccaagtg cgccacgaag cgcgacttcg ccgaagggac tagcaagcct cccccaaccc
     2161 ccgcgaggat cagccctgca gtgacggaaa ttaaactctt ccacgcgcgc tgccgtcctg
     2221 aaatgattgc gtagccaagg agcgccccgt agccggccac caggaaactc aggccataaa
     2281 ttcccgtcac actggcgaat tgaaccaagc ccaggctgct gcttaccgcg tagcccgcca
     2341 gattccacgg gaatccaatg atcggcaaat gtgcgcgcgc gaattcgagc gtcacccaca
     2401 gacaaggtgc gagtacgcag gccagcagtg cgctccggcg cgacgccaac gcaatcccca
     2461 gcgaaaatgc tgcgagaata attccaccgg cgatagcaat cagtgcaaga aggcccaccg
     2521 cagtcaaagg aggtacatcg ccatactggc gcactactac gtcgatccat gtaaggcaca
     2581 acggatagaa aacgagtgcg tgaagaaaac cgtagagcgg ggccagcgcg ggtctggctt
     2641 gataggatgc gaggaccaac agtgcaatcg agatccacgc aaggagcgag aaattaaagt
     2701 tggggaagga gagcgccaat gccaggccgc tcgcaagcgc gagcaatacc cgcgtgtgcc
     2761 agggccagtt catttcagag gggtcattca gcgtttgata atagacttga aaccggcgcg
     2821 ctcaagcgcg ttcctggcgg attcggcggt tcgctgatct cgatagggac cgacttgcac
     2881 gcggtaaaaa ttatcgccgg atggcggaag aacaaaagcg gaaaatttct ttcgctgcaa
     2941 cgcgtcggcc attgccagcg cgtcgctttg gtgcgtcacg gcggcgacct ggagcaccac
     3001 ggaccctttc agaattctcg gaggctggaa ccgcgacgat acgggtatag ccttggcgct
     3061 ttccgtcgcc gcctgcggcg ctgaagtatc ctttggggcc ggcgcggagg ttgggatcgc
     3121 cggagatgac ggcttcccgg ccggctccag acggttgctg gcattttttg agtagaaatc
     3181 ccattcgttg ttagccggcg cggcactggg cgcgctatta gaagcggccg ccttcacagc
     3241 cttatttgcg aggagtgcgc tcgcttcagg tgcttccgcc gcgtgtaccg ccccatcgta
     3301 ttggttgtgg ccgatcacgt accctaatgt gaagaaaacg ccgcacagca gtacgatgcc
     3361 aagaaacaag acgattaagt gccggctttc gagcactcgg tctccgcgtt ttcctccatt
     3421 gcccattcgc tacttgtttc ctcccagagc cttcatccac tgcgacatca tatcgatcgg
     3481 tagcggaaag atcaccgtgg tattcttctc tacgccaatt tcggtgagcg tctgcagata
     3541 ccgtaactga atcgaaacag gttc
//
LOCUS       DATNDT010000034         2348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_801573, whole genome shotgun sequence.
ACCESSION   DATNDT010000034 DATNDT010000000
VERSION     DATNDT010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2348
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_801573"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1326
                     /locus_tag="VN788_01195"
     CDS             <1..1326
                     /locus_tag="VN788_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family protein"
                     /protein_id="HXU19177.1"
                     /translation="PKFFSGENPELAGYIRQLGKSGVLRIGGNTSEYCYWKPIAERAA
                     SGNTESISADKTNPVAFGLAVGPDKGLTASAAVSITPLAVRNLRDFLDACGWKLIYGL
                     NMGTGTPEAAADEAAYVMDTIGAKLIAFQLCNEPDLFYRNKIRKSDYDFAQFADEWQR
                     FYQAIRARVPKAAFAGPDTAYNNEWLVPFARRFKDEAVFLSQHYYAEGPPTDPSMTIE
                     RLLRPNPALEAEFAGMRKTVQESGLPFRLAETNSCYRGGKLGVSDTFASALWAADLMY
                     QLASAGGMGINFHGGGYGVYTPIAGTREDGFLARPIYYGMLLFAQAGVGQLVESNLGS
                     DAGSQAPLLTVYGLRSDSGVIKAAVFNKNLDRGVRLTIDPGERSQRASALRLHAPRID
                     DTTDATFGGSVVGASGAWSAAHEEKLSKENGAAVIDLPAASAALVVFER"
     gene            1589..2236
                     /locus_tag="VN788_01200"
     CDS             1589..2236
                     /locus_tag="VN788_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19178.1"
                     /translation="MGTLGPAAESLRLAEHYRRLSDDELIELAQDASELTELAQGALK
                     QEILERKLKVPAKEPERPQPEPQFSESEDEYAEDRSLVELCTVWSRNDALKVQELLSA
                     VDIPFFMGPERATTVDKITSRFSDGVPVGVMQIGFPWAHEAIRNYVPKDAPPEPNDDA
                     EDADIRCPKCHSTDVVFEHLSDDSKDGKSSTSQRFDWTCASCGHSWEDDGVESNG"
BASE COUNT          554 a          635 c          683 g          476 t
ORIGIN      
        1 ccgaaatttt tttctggcga gaatccagaa ctcgctggat atatccggca gttggggaaa
       61 tccggcgtgc tgcgcatcgg cggtaatacc agcgaatatt gctactggaa gcccatcgct
      121 gaaagagctg cgagcgggaa taccgaaagt atctccgcgg acaagaccaa cccggtcgcc
      181 ttcggactgg ccgtcgggcc tgacaaagga ttgaccgcgt ctgctgcggt cagcattacg
      241 ccgctggcgg ttcgcaactt gcgggacttc ctcgatgcgt gcggctggaa actgatttat
      301 ggattgaata tgggcacggg cacgcccgaa gctgccgccg atgaagccgc gtacgtaatg
      361 gatacgatcg gcgcgaagct gattgccttt cagctatgca atgagcctga tcttttctat
      421 cgcaacaaga ttcgcaaatc cgactatgac ttcgcgcaat tcgcagatga gtggcagcga
      481 ttttatcaag ccattcgcgc gcgggttcct aaagcggcct tcgccggacc cgataccgcc
      541 tataacaatg aatggctcgt gccattcgcc aggcgcttca aggatgaagc cgtgttcctc
      601 tcgcagcact actacgccga agggccgccg accgatcctt ccatgaccat tgaacgtctg
      661 ctgcgcccga atcccgcgct ggaagcagag tttgccggaa tgagaaaaac ggtgcaagaa
      721 tcgggactgc cctttcgatt agctgaaacc aactcctgtt atcgcggcgg caagctagga
      781 gtgagcgaca cgtttgcttc ggctctctgg gccgcggact tgatgtatca gttggcgtcc
      841 gcaggaggca tgggcatcaa cttccacggc ggcggctacg gcgtgtacac accaatcgcc
      901 ggcacccgcg aggatggatt tttggcccgt cctatttatt acggcatgtt gctgttcgcg
      961 caagcgggcg ttgggcaatt agtggaaagc aacctgggtt cagacgctgg ttcccaagcg
     1021 cccctgctca cggtatatgg actgcgaagc gatagcggcg tgatcaaggc cgcggtgttt
     1081 aataaaaacc tagatcgagg cgtgcggctt acgattgatc ccggagaacg atctcaaaga
     1141 gcgagcgcgc ttcgcctgca cgctccacga atcgatgaca ctaccgacgc caccttcggc
     1201 ggctccgtcg tcggcgcgag cggagcatgg tccgctgcgc atgaagaaaa gttatccaag
     1261 gagaatggtg cggcggtgat tgatttgccc gcagcgagcg ctgcgttggt tgtgtttgaa
     1321 cgctagcgac aacgatcagg gaatcgagcc acccaatgct atccccaccg ccggccacgg
     1381 accatgatca cctcctaaat catgttatct cgacgttgag cgtagcgaag acggagagac
     1441 cttatgtgtg tttgaagcca tcgatgtaga caccaggacc ataaaccctg tccgcatcaa
     1501 gaatggtttc gagtgttagg acggcacaca ctcaagggca aggggcttgc gaagccaacc
     1561 tgaaaacatt taccttaatg cgaatctgat gggcacacta ggtccagctg cggagtcatt
     1621 gcgcctcgcc gagcactatc gccggctgag cgatgacgaa ctgatcgagc ttgcgcaaga
     1681 tgcgtctgag ctgaccgaac tggcacaggg agctttgaag caggaaatcc tggagcggaa
     1741 actcaaggtt cccgcgaaag agccggaaag gccgcaaccc gagcctcaat tcagcgagtc
     1801 cgaggacgag tatgccgaag atcgcagcct tgtagaattg tgcaccgtat ggagccgcaa
     1861 cgacgccttg aaggtgcagg aattgctcag cgccgtagat atcccctttt tcatggggcc
     1921 agagagggca acaacagtgg acaaaatcac gtcaaggttc tcggacgggg ttccagtggg
     1981 agtcatgcaa atcggcttcc cctgggcgca cgaagcaata agaaattatg tgccaaagga
     2041 cgcgcctccc gaacctaacg acgacgctga agacgccgac attcgttgtc cgaaatgcca
     2101 ttcgacggat gtggtgttcg aacatctaag tgacgactcg aaagatggca aaagttcaac
     2161 gtcgcagaga ttcgattgga cctgcgcctc ttgcggacat tcatgggaag acgatggtgt
     2221 ggagagcaat ggctagtgca atgattcgcg aatgcttcta ctccccatgc cgtcgcggca
     2281 cccgcttgga aatattgcag aggatttcat aagggacggt attcgccagc gacgcgtgct
     2341 cgcgagca
//
LOCUS       DATNDT010000035         6443 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_797996, whole genome shotgun sequence.
ACCESSION   DATNDT010000035 DATNDT010000000
VERSION     DATNDT010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6443
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797996"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1190
                     /locus_tag="VN788_01205"
     CDS             153..1190
                     /locus_tag="VN788_01205"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HXU19179.1"
                     /translation="MKRTALPTALVSAAVLLIGISLQASRQAAQQIPSRHFELSYIVH
                     VPALPQGSHQLRLWIPLPYQDAYQSVSHLRIESPVTYKIDHQPEYSDRYAYLTVSGVQ
                     ARKSFDIKISSDVVRREHRVSLTPSSNPSSTPHVETARYLRPDRLVPINGQIAELSQE
                     QTEGATLPLEKVRKIYNYVIASMHYDHEGTGWGHGDAIYACTAKHGNCTDFHSLFIAM
                     ARAAGIPARFEIGLPLPPGPAHADITSYHCWAEFYIKGIGWVPIDASEAWKHKEKINY
                     FFGAIDTNRVMFSLGRDIRLSPAQAGDPLNYFVYSYAELDGKPFEDLKSDYSFRDTAA
                     PAPVSHGAGAN"
     gene            complement(1266..2120)
                     /locus_tag="VN788_01210"
     CDS             complement(1266..2120)
                     /locus_tag="VN788_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXU19180.1"
                     /translation="MVISFKSIAEGELFIPGNMDLYNAAYANYETDAYRQVRIETYGE
                     DFGQTSWVTTDESREIPKLLGLTSHAVVLEIGCGSGRYALQIAESVGCRIFGVDVNGS
                     GIDNANQLAKQRSLAGQASFELCDASKKLPFDDATFDAMFSNDVLCHIPGRLAVLREI
                     FRVLKPGARILFSDALIIGGIISHQEIATRSSIGYYNFSPPGENERLIEQAGFRLLKS
                     SDTTSHAARIAKRWHDARERRKSELIAAEGSANFEGVQQFLSCVHTLTSERRLLRYIY
                     LAQKADQI"
     gene            complement(2136..3044)
                     /locus_tag="VN788_01215"
     CDS             complement(2136..3044)
                     /locus_tag="VN788_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXU19181.1"
                     /translation="MRIFPPGLFQRATVPLIVAMIALLSGCTMRPKDTIVVGSKNFPE
                     QALLGEILAQHLEARTHLHVQRRFYLAGSFICQQALLAGRIDTYVEYTGTALTAILHD
                     PIESGSSAVFDKVKAEYKQRFGFEVMPSLGFNNTFAIVIRGDDARRLHLTTLSQAAQY
                     TPQWRAGFGYEFMERPDGYPGLARVYGLRFASPPQIMDLGLLYRALLSKQVDLVAGNS
                     TDGLLSARDLVILQDDKHYFPPYEAVPITRQDLFARYPEARTAIAELAGKISDAEMRK
                     MNYEVVGQSRDASEVARDFLHSKNLD"
     gene            complement(3052..3681)
                     /locus_tag="VN788_01220"
     CDS             complement(3052..3681)
                     /locus_tag="VN788_01220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19182.1"
                     /translation="MMDFLSRHGSEILQATTEHVWLVAATMLLSIAIGVPLGILVARR
                     PWLSKPILGSANIAETIPSLALLGFFLTVPWLGARADRLTIAALTLYALLPIIRNTAT
                     GIAAVDPAVREAARGMGMTERQILFHVELPLSVSTVLAGIRVATVLTIGIATIAAAVG
                     AGGLGEFIFRGLAMVNNPLILAGAVPAAVLALAADFLLSLLERRLRPAR"
     gene            complement(3697..4473)
                     /locus_tag="VN788_01225"
     CDS             complement(3697..4473)
                     /locus_tag="VN788_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013325424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU19183.1"
                     /translation="MSAHSEASPQRDAIIEFRDAAYRLENGQLLLSRINLTVRRGETL
                     VLLGRSGAGKTTALKLINRLLDPTEGEVRVEGCRTTEWNPIDLRRHIGYVIQETGLFP
                     HYTVEQNISIVPRLEQWPRERARARARELLALTGLDPNLFLSRYPHELSGGQRQRVGV
                     ARALAADPPILLMDEPFGALDPLTRAEIRGEFQALQRHLGKTIVIVTHDTNEALLLGT
                     RIGLVEAGELKGMYSPHEFMRSNEPVAAAYAAQLRVFNGI"
     gene            complement(4470..5168)
                     /locus_tag="VN788_01230"
     CDS             complement(4470..5168)
                     /locus_tag="VN788_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXU19184.1"
                     /translation="MTGTVKKLNEEIVRCRKCPRLVKYREAVARVKRPAFRNWDYWGR
                     PVPGFGDSRASLVILGLAPAAHGANRTGRMFTGDRSGDFLYKALYGAGFANQASSISR
                     DDGLALNNAYISATLRCAPPANKPLPSELVNCKPYFETELKLIRPSAVLALGGIAMRA
                     YLLLLKQQGAIEQPSAFTFRHSASYDLGPGLPRLFVSYHPSQQNTFTGKLTEAMLANV
                     LQDIRRFLEKKSPL"
     gene            5673..>6443
                     /gene="trpC"
                     /locus_tag="VN788_01235"
     CDS             5673..>6443
                     /gene="trpC"
                     /locus_tag="VN788_01235"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018871812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HXU19185.1"
                     /translation="MEISSREKTILDWIVEARRKSIAHRKRSLPEAALKFAVEKAPQS
                     RDFAAALSRNTFNVIAELKKASPSRGILRENYAPGTLAASLESAGAAALSVLTEEDFF
                     SGSLGDLKEAKKAVQIPILRKDFIVDPWQVLEARAAGADAFLLIVAILDDARLRELLE
                     LGRSLKMEPLVEVHSREEIARAVTAGAHIIGVNNRDLRDFSVRLETSLDLIEVIPEDC
                     IAVSESGLRTHEDLARLRRAGFDAFLVGEHLMKDADPAV"
BASE COUNT         1392 a         1886 c         1813 g         1352 t
ORIGIN      
        1 ctttcgccac gaaagccggg ttcgacaacc tgccgggcct agtctgcaac ccctctttgg
       61 tggcataatc ctgccatcgc gaacgcgccg gtcgctaacc tgggttcgac ggtcgcggag
      121 gtcaatatga acatcaaaag gaaactaaaa acatgaaacg aactgcgctt cctaccgcgc
      181 tggtcagcgc ggccgtgctg ttgatcggaa tttccctcca agcttctcgc caagccgctc
      241 agcagattcc ttcccggcat ttcgagcttt cctatatcgt tcacgttccc gcgcttcctc
      301 agggatccca ccaactaaga ttgtggattc ctcttcctta ccaggacgcc tatcagtcgg
      361 tttcgcacct ccgcatcgaa agtccggtga cctacaaaat agatcaccag cctgaatata
      421 gcgaccgcta cgcctactta accgtaagtg gcgtgcaagc gcggaaatca ttcgacatca
      481 aaatttcgtc cgatgtcgtt cgccgcgagc atcgcgtctc gctgacgcct tcaagtaatc
      541 cctcgtcgac gccccatgtc gagaccgccc ggtacctgcg tccggatcgc ttggtcccca
      601 tcaacgggca gattgccgag ctttcccagg aacaaaccga aggcgccact ctgcctctcg
      661 agaaggtgcg caagatctac aattacgtga tcgcttccat gcattacgat catgagggca
      721 cgggttgggg gcacggcgac gccatatacg cctgcacagc caagcacggc aattgcaccg
      781 acttccattc gctcttcatc gccatggccc gcgctgccgg cattccggcg cggttcgaaa
      841 tcggtcttcc gctgccgccc ggtcccgctc acgcggacat caccagctac cattgctggg
      901 cggagtttta catcaaagga atcggatggg ttccgatcga cgcctccgag gcctggaagc
      961 acaaggagaa gatcaattac ttcttcggag cgatcgatac gaaccgcgtt atgttcagtt
     1021 tgggccgcga cattcgcctc tctcccgcac aggctggcga cccgttgaac tacttcgtct
     1081 attcctacgc ggagctggat ggcaagccat tcgaggattt aaagagcgat tattcgttcc
     1141 gtgacactgc cgctcccgca cccgtttctc acggcgctgg agcaaattaa agggaggggc
     1201 gtcggaagct ggcgcctctc gatggttcca agttatttgg cggcttgaac gcggaagaac
     1261 tgcaattaga tctggtcggc cttctgtgcc aggtagatat accgcaacag gcgccgctcg
     1321 ctcgtaaggg tgtgcacgca ggaaagaaac tgctgtactc cttcgaaatt cgccgaaccc
     1381 tccgccgcga ttagttcaga tttcctgcgc tcgcgagcgt catgccaacg cttcgcaatc
     1441 cgagcggcgt ggctagttgt gtcgctcgat tttaggagac gaaacccggc ctgttcgata
     1501 agacgctcat tctcaccagg cgggctgaag ttgtaatagc ctattgagct gcgagtagca
     1561 atctcctggt gcgaaatgat tccgccgatt attaaggcat cgctgaatag aatccgtgcg
     1621 cccggcttca ggacgcgaaa aatctcgcga agcaccgcga gccgtccagg tatgtgacac
     1681 agaacatcgt tcgaaaacat tgcgtcaaac gtcgcatcgt cgaatggaag cttcttcgag
     1741 gcgtcgcaca gctcgaaact cgcttggccg gccagactgc gctgcttcgc gagttggttg
     1801 gcattatcga tccctgatcc atttacatcc acgccgaaga tgcggcagcc gactgattcc
     1861 gcgatctgca aagcatatcg tcctgacccg catccgattt ccagaacgac tgcgtgggat
     1921 gtcagtccca ggagctttgg gatttcgcgt gactcgtcgg ttgttaccca acttgtctgc
     1981 ccgaaatcct ctccataggt ctcaatgcgg acctgccggt acgcgtccgt ttcataattg
     2041 gcgtaagcgg cgttatagag gtccatattc cccggtataa aaagctcccc ctcggcgatg
     2101 gacttgaagg atatcaccac gtccgggcct tcgagttagt cgaggttttt ggaatgcagg
     2161 aagtcgcggg ccacctcgct cgcgtctcgg gattggccga ccacttcgta attcattttt
     2221 cgcatttcgg cgtcggagat tttcccggct agttcggcta tggccgtgcg ggcttccgga
     2281 tacctggcaa acagatcctg gcgcgtgatg gggaccgcct cataaggcgg gaagtaatgt
     2341 ttgtcgtctt ggaggatgac caaatcgcgc gcggacaaca atccgtccgt agaattaccc
     2401 gccacaagat cgacttgctt ggagagcagc gcgcggtaaa gcaggcccag gtccatgatt
     2461 tggggcggac tagcaaaccg caatccatat acgcgcgcca ggccgggata tccgtcgggc
     2521 cgttccatga actcgtatcc aaatccagcg cgccattgcg gagtgtattg cgctgcttgc
     2581 gacagagtcg tcagatggag gcgacgcgca tcgtcaccgc gaattacgat ggcaaatgta
     2641 ttgttgaagc cgagcgatgg catcacctca aatccaaacc gctgcttgta ctccgctttc
     2701 actttgtcga ataccgccga cgatccgctt tcgatcggat cgtgcagaat ggccgtaagg
     2761 gctgtgccag tgtattcgac gtacgtatca atgcgtcctg cgagtaaggc ctgctggcag
     2821 atgaagctgc ccgcgagata aaaacgccgc tggacgtgca aatgcgtgcg cgcttcaaga
     2881 tgctgcgcca gaatctctcc gaggagcgcc tgttctggaa aattcttcga tccaaccaca
     2941 atagtatctt tcggccgcat cgtgcagcct gaaagcagcg cgatcatcgc gacaatcagc
     3001 ggaacggtgg cgcgctggaa gagtcccgga gggaatattc tcacttgaac ctcatcgcgc
     3061 gggccgcaag cgtcgttcca acaagctgag caggaaatca gcggcaagtg cgagcacagc
     3121 tgccggaacc gctccggcaa gaatcaacgg attattcacc attgccaggc cgcgaaaaat
     3181 aaactccccg aggccgcctg ctcccacagc ggccgctatt gtagcgatgc cgatggtgag
     3241 cacggttgcc acgcgaatgc ccgccagcac ggtcgaaacg gaaagcggca gttccacgtg
     3301 aaacaaaatt tgccgctcgg tcattcccat gcctcgcgcc gcttctcgaa ccgctggatc
     3361 cacagcagcg attccggtcg cggtattacg aatgatcgga agcagggcgt aaagcgtgag
     3421 cgcggcaatc gtcagccggt ccgctcgcgc gccgagccac ggaaccgtaa gaaagaaacc
     3481 cagcagcgcc aggctgggaa ttgtttccgc aatgttggcg cttcccagga taggcttgga
     3541 aagccacggg cgccgcgcca ccaaaatccc cagcggcacg ccgatggcga tcgaaaggag
     3601 catggtcgcg gcgaccagcc agacgtgttc ggtagtcgcc tgcaggattt cgctcccatg
     3661 ccgtgataaa aaatccatca ggctcatggt tccctctcaa attccgttaa aaacgcgcag
     3721 ttgcgcggcg taagcggcag ccacgggctc gtttgatcgc atgaactcgt gtggtgaata
     3781 cattcccttg agttcaccgg cttcgaccag tccgatgcgc gtgcctagca agagcgcctc
     3841 attcgtgtcg tgcgtcacaa tcacgatcgt tttgcccagg tgacgttgca gcgcctgaaa
     3901 ttcgccgcga atctctgcgc gggtcagtgg gtcgagggcg ccaaaaggct cgtccatcag
     3961 cagaatcgga ggatcggcgg ccagcgcccg cgccactccc actctctggc gctggcctcc
     4021 tgaaagttcg tgcggataac gcgagagaaa aagattcgga tcaagcccgg tcaaagccag
     4081 caattcgcgc gcacgagcgc gagcgcgttc gcgcggccac tgttccaagc gaggaacaat
     4141 ggaaatattc tgctcgacgg tatagtgcgg aaataatcca gtctcctgga tcacgtagcc
     4201 gatgtggcgc cgtaggtcta tagggttcca ttcggtggtc cggcaccctt ccacgcgcac
     4261 ttcgccctcc gtgggatcga gcaagcgatt gatgagcttc aaggcggtgg tctttcctgc
     4321 gccgctgcgg cctagaagca ccaaagtttc gccccggcgt accgtcagat tgatgcgact
     4381 caataacaat tgtccgtttt caagcctgta cgcggcatcg cgaaattcga tgatcgcgtc
     4441 gcgctgagga ctggcttcgg aatgggcgct catagcggcg atttcttttc caggaatctg
     4501 cggatatctt gcaagacatt cgcgagcatg gcttcggtga gctttcccgt aaacgtattt
     4561 tgttggctgg gatgataaga gacgaatagc cgcggcagac ccggtccgag gtcgtagctt
     4621 gcactgtgcc ggaaagtgaa cgcgctaggc tgctcgatcg cgccttgctg tttcagaagg
     4681 agaagatacg cgcgcatagc gattccgccc agagcgagca cggcggacgg ccgaatcagc
     4741 ttcaattccg tctcgaaata cggcttgcaa ttcaccagtt ccgacggaag aggtttgttc
     4801 gcgggcggcg cgcaccggag cgtggccgaa atgtatgcat tattgagtgc cagcccgtcg
     4861 tcgcggctaa tcgaagatgc ctggttcgca aatcccgcgc cgtatagggc tttgtagagg
     4921 aaatctccag agcgatctcc cgtgaacatc ctgccagtgc ggttggcgcc gtgcgcagcg
     4981 ggagctagtc ccaggatcac aagagacgcg cgcgaatcgc caaacccggg caccggtctg
     5041 ccccaatagt cccagttgcg aaaggctggg cgcttcacgc gagcaacggc ttcccggtat
     5101 ttcacgagcc gggggcactt ccggcaccgc acaatttcct cattgagttt tttaactgtt
     5161 ccagtcatcg gttgtagctc atcccgccgt cgcagggtga ggattttcgg ctcttcggac
     5221 tcaccgagaa gctaccacga atctctcaga ctcactcgat gagaaaaagt ccacaggagc
     5281 cggttcttac aaaggttttc tacggtgcta acgtgcccga aaggagaatc gtgatatttg
     5341 gagacaagct caatcgcaac gcccgcttgc cccgagcgaa tctgaagact gtgtcgccgg
     5401 agcctttgaa tccgcctagc cgtcgtttgg cctcgcccca gcgctagatc ctgctcgaca
     5461 catcggtcga gtagaattcg atgtaacttg ctgaaaacac ggctccgatg tcaccgctac
     5521 ccgtcacact ctaaagagtc tgaaagaccc ctaatgcggc tccgccaaat tgacagcgcg
     5581 ggaaggcgcg gatagcatga tggaagcagc ctcgcatccg cataaacgaa ggcgtagctc
     5641 gcgacgcgcg gaattggccc gcgcccaagc ctatggagat tagctcacgc gaaaaaacaa
     5701 tcctcgactg gattgtcgaa gctcgccgga agtcgatcgc gcaccgaaag cgctcccttc
     5761 ctgaagctgc gctgaagttc gcggtggaaa aagccccgca gtcgcgcgac ttcgccgccg
     5821 ccctctcccg caacaccttc aacgtgatcg ccgaactgaa aaaggcttcg ccctctcgcg
     5881 gcattctacg cgagaattac gcgccgggta cgctggccgc gagcctcgaa tcggcggggg
     5941 ccgcggcgct atcggttctc actgaggaag attttttttc cggatcgctg ggtgatttga
     6001 aagaggccaa aaaggcagta caaatcccca ttttgcgaaa ggattttatc gttgatccgt
     6061 ggcaggtgtt ggaagcgcgg gccgctggcg ccgatgcgtt tctcctgatc gtagcgattt
     6121 tggatgacgc gaggctacgc gaattgctcg aactcggccg ttcactgaaa atggaaccgc
     6181 tggtggaagt gcattcgcgc gaagaaatag cacgcgcggt gacagcggga gctcacatta
     6241 tcggcgtcaa taatcgcgat ttgcgagatt tctcggttcg gctcgagact tcgctcgacc
     6301 tgatcgaagt gataccggag gattgcatcg ccgtgagcga gtccggcttg cggacacacg
     6361 aggatttggc ccgcctccgg cgcgcgggct tcgatgcgtt tctggtaggc gagcatctga
     6421 tgaaagatgc cgatccggct gtg
//
LOCUS       DATNDT010000036         6053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3091317, whole genome shotgun sequence.
ACCESSION   DATNDT010000036 DATNDT010000000
VERSION     DATNDT010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3091317"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1872)
                     /locus_tag="VN788_01240"
     CDS             complement(<1..1872)
                     /locus_tag="VN788_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HXU19186.1"
                     /translation="MSNRNGNRVIRERERLDFAKIQTAVQIPNLIDVQKKSYQRFLQM
                     DLLPSEREDTGLQSVFASVFPIQDFRGLSQLDFVDYAIGNWECKCGHLRGLHHLRATC
                     RNCGASVVTNPYKTGDVICAKCGTFNKNTPTFCNKCGDPVTLQLKYDVTECQERGMTY
                     SAPLKVTIRLTIYDKDPDTGAKSIRDIKEQEVFFGDIPLMTENGTFVINGTERVIVSQ
                     LHRSPGVFFETANNRSYFLGKIIPYRGSWVEFEYDTKNILYVRIDRKRKFLGSIFMRA
                     LGMKSNEDILRTFFQVERVSVRDRDLFLNVSEGLIDRKLSREIKHPKSGEVIVGSHKR
                     ISEPLYQELMKARVVQAEVTPADLEGAVTVSELVNRQTGEVLLEANKPVTAEMWTAIG
                     EAGVTEVDVCFPDRDDVGIIISRTLEKDTIHTPKEALIEIYRKLRPGDPPTLETATAL
                     FNGMFFDARKYDFSKVGRLKFNIKLGLETPLDKRTLDPSDFVAAIKYLLKLRKNIGTV
                     DDIDHLGNRRVRAVGELLENQFRIGLVRMERAIKEKMSVYQEMSTAMPHDLVNAKPVM
                     AAIREFFGSSQLSQFMDQTNPLSEITHKRRLSALGPGGLSRERAGFEVRDVHPTHYGR
                     "
     gene            complement(2038..2424)
                     /gene="rplL"
                     /locus_tag="VN788_01245"
     CDS             complement(2038..2424)
                     /gene="rplL"
                     /locus_tag="VN788_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HXU19187.1"
                     /translation="MASKVEGILEEIKGLTLLEASDLVKKMEEAFGVSAAAAAPVMMA
                     GGAGAGAAAGPAEEKTEFTIVLTEVGANKINVIKAVREVTSLGLKEAKDLVDGAPKTV
                     KEGINKDEAATIKKKFEDAGAKVEIK"
     gene            complement(2478..3062)
                     /gene="rplJ"
                     /locus_tag="VN788_01250"
     CDS             complement(2478..3062)
                     /gene="rplJ"
                     /locus_tag="VN788_01250"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HXU19188.1"
                     /translation="MRKKAEKKAQGEELRAQLATVSTVILSTFQGITVEQDTKLRRSV
                     EAAGAHYEVVKNTVAERAAAGTPAEGLLKDLKGTNSIAYTKTDPVALAKILSKVAKDV
                     PAFKFRAGWVEGRVISIQEISQLAQLPSKEELISKIMFMLNAPAQRVATALAALPRNL
                     AVVISEAVKANKFGGGPASEAATGTQTAPEPEKA"
     gene            complement(3093..3815)
                     /gene="rplA"
                     /locus_tag="VN788_01255"
     CDS             complement(3093..3815)
                     /gene="rplA"
                     /locus_tag="VN788_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HXU19189.1"
                     /translation="MGRGAKVGGKNIDRARKLVEARPYKLEEAAELLRKAHFVKFDET
                     VELVLNLGVDPKQSDQMVRGTVVLPHGLGKSKRVLVIASGEKVREAEEAGADFVGGED
                     MVQKITEGWTDYEAVIATPDMMKSAGRLGKVLGPRGLMPNPKTGTVTFEVAKAVKEIK
                     AGKVEFRLDKTGLIHSPVGKISFPAKNLAENAQALLGAVVKAKPSAAKGKYIKKITLT
                     STMGPGVSIDHTEAESATAVAA"
     gene            complement(3828..4256)
                     /gene="rplK"
                     /locus_tag="VN788_01260"
     CDS             complement(3828..4256)
                     /gene="rplK"
                     /locus_tag="VN788_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HXU19190.1"
                     /translation="MAKKVQAMVKLQLPAAKATPAPPVGTALGPHGVNIMDFCKQFNA
                     KTSKEPDGMIFPVLVTIYSDRTFTFILKTPPASVLLKRAAGIVKGSPEPNRNKVGKVT
                     RKQVEEIAKTKMPDLNTADIESAMRSVMGTARNMGIEVAE"
     gene            complement(4380..4913)
                     /gene="nusG"
                     /locus_tag="VN788_01265"
     CDS             complement(4380..4913)
                     /gene="nusG"
                     /locus_tag="VN788_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020263434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HXU19191.1"
                     /translation="MSKQWYIVHTYSGFEKKVKESLEGRVQAFGLTDKIGQVVIPTES
                     VVEVRGGKKVVSPRMCYPGYVLVEMDMDDYTWHVVRSTPKVTGFVGSGQMPSPLTEEE
                     VQNILNRATTTAEKPKPKLSFERGEKIRIADGAFANFNGVVEEVNTDRSTLKVSVTIF
                     GRSTPVELDFAQVEKVA"
     gene            complement(4924..5214)
                     /gene="secE"
                     /locus_tag="VN788_01270"
     CDS             complement(4924..5214)
                     /gene="secE"
                     /locus_tag="VN788_01270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HXU19192.1"
                     /translation="MADAARTNRDEESHRVSGFSLMEPAQKLGGYPKRLRNFLHDVRV
                     EMKQVNWPSRSDVWSTTVVVTITVAFFGVFFWITDGIFGRLYILVNSYFHHH"
     gene            complement(5275..5349)
                     /locus_tag="VN788_01275"
     tRNA            complement(5275..5349)
                     /locus_tag="VN788_01275"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5314..5316),aa:Trp,seq:cca)
     gene            complement(5350..5712)
                     /locus_tag="VN788_01280"
     CDS             complement(5350..5712)
                     /locus_tag="VN788_01280"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXU19193.1"
                     /translation="MADGDEPWFCYMVRCNDGSLYVGIARDVAERVKRHNWGVGPGFT
                     AKRRPVGLVWSERCDGSKVARAREKEIKGWSRSRKLALIEKNGEGQPVALRMGRVNPS
                     SLRTAGKPFKPANGGFSG"
     gene            complement(5723..5872)
                     /gene="rpmG"
                     /locus_tag="VN788_01285"
     CDS             complement(5723..5872)
                     /gene="rpmG"
                     /locus_tag="VN788_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HXU19194.1"
                     /translation="MREIITLQCNDCKNRNYTTTKNRKKHTERLETQKFCNTCRKHTG
                     HKEVK"
     gene            complement(5879..>6053)
                     /gene="tuf"
                     /locus_tag="VN788_01290"
     CDS             complement(5879..>6053)
                     /gene="tuf"
                     /locus_tag="VN788_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349098.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HXU19195.1"
                     /translation="VTGDVKLPTGVEMVMPGDNVSAEITLITPVALEKGLRFAIREGG
                     HTVGAGAVAEIVE"
BASE COUNT         1151 a         1915 c         1494 g         1493 t
ORIGIN      
        1 gcgcccatag tgcgtgggat gcacatcgcg cacttcgaat cccgcgcgct cgcgagaaag
       61 gccgccgggt cccagcgccg aaaggcgccg tttgtgcgtg atttcgctca gcggattggt
      121 ctgatccatg aattgtgaaa gttgcgagct gccgaaaaat tcacggatcg cggccattac
      181 cggcttcgca ttcaccaggt cgtgcggcat cgccgtggac atttcctggt acacggacat
      241 cttttcctta atggcgcgtt ccatgcgcac tagcccgatg cggaactggt tctcgagcag
      301 ttcgcccacc gcgcggacac gacggttgcc aagatgatcg atgtcgtcca ccgtgccgat
      361 gttcttgcgc agcttcagca gatacttaat tgccgcaacg aaatcggacg gatcgagcgt
      421 ccgtttatcg agcggcgtct ccaaccctag cttgatattg aacttcagcc gccctacctt
      481 gctgaagtcg tacttgcgcg catcgaaaaa cattccattg aaaagcgctg tggcggtttc
      541 aagcgtcggt ggatctcctg gacgcagctt gcggtagatt tcaatcagcg cttccttggg
      601 agtgtggatc gtatccttct caagcgtacg gctgatgatg atccccacat cgtcccggtc
      661 cggaaaacag acgtccactt cagtgacgcc agcctcgccg atcgccgtcc acatttccgc
      721 ggtcaccggc ttattggctt cgagtagcac ctcgccggtt tgccggttta ccagttcgct
      781 caccgttacc gcgccctcca gatcggcagg cgtgacttcc gcctgaacca cgcgcgcctt
      841 catcaactcc tggtaaagcg gttcggaaat acgcttgtgc gagccgacaa tcacctcgcc
      901 ggacttcggg tgtttgattt cacgcgacag cttgcgatcg atcaggccct cggagacgtt
      961 caaaaaaaga tctctgtcgc gaaccgaaac gcgctctact tgaaaaaagg ttcggagaat
     1021 atcctcattg gacttcattc ccaaagcgcg catgaaaatc gaaccgagga atttgcgctt
     1081 gcggtcaatt cgtacgtaga gaatgttctt agtgtcgtat tcaaactcaa cccacgatcc
     1141 gcgatacgga ataatctttc caaggaagta gctgcgattg ttcgccgtct cgaaaaacac
     1201 cccgggtgaa cggtggagct ggctaacgat cacgcgctcg gtgccgttga tgacgaaagt
     1261 gccgttctcc gtcatcagcg gaatatcccc gaaaaagacc tcctgctcct tgatatctcg
     1321 aatcgatttt gcgccggtgt ccggatcctt gtcatagatg gtgagccgaa tagtcacctt
     1381 caatggcgcg gaataggtca tcccgcgttc ctggcactcg gttacgtcgt acttgagctg
     1441 gagcgtgaca gggtcgccgc acttgttgca gaaagtgggc gtatttttgt tgaatgtgcc
     1501 gcacttcgcg cagatgacgt cgcccgtctt ataagggttg gtgacgaccg acgcgccgca
     1561 attccggcag gtggcgcgca aatggtgcag accgcgcaaa tggccgcatt tgcactccca
     1621 gttgccgatc gcgtagtcga cgaaatccag ctgcgatagg ccgcgaaaat cctgaatcgg
     1681 gaaaacggac gcgaacaccg attgcaatcc cgtgtcttcc cgctcgctcg gcagtaaatc
     1741 catctgcaaa aatcgctgat aggatttttt ctgcacatcg ataaggttgg gtatctggac
     1801 cgccgtttga atcttggcga agtccaagcg ttcccgctcc cgaatcactc gattaccgtt
     1861 acgattgctc atgggccatc ctcttcaaga ctagtgccgg gataacaatg cgggtggaca
     1921 cccgccctat cagcccggga aggacgtgtt ttgaggttct atgttctgaa taccgaagga
     1981 gcgcgtgcgt acgcgaattc ctttgcagcc cggcacaggg ccgtttacag gctgccacta
     2041 cttgatctcg accttggctc cggcgtcttc gaatttcttc ttgatcgtgg cggcctcgtc
     2101 cttgttgatg ccttccttta cggtcttcgg agcgccatcc acaagatcct tggcttcttt
     2161 caagcctagg ctggtgactt cgcggacagc cttaatcacg ttgatcttgt tcgcgccaac
     2221 ttcggtgaga acgatggtga attccgtctt ctcctccgcc ggaccagccg ccgcgccagc
     2281 gccggcgcca cccgccatca tcacgggagc ggccgccgca gccgagactc cgaaggcttc
     2341 ttccatcttc ttgaccagat cggaagcctc cagcagcgtc agacctttga tttcttccaa
     2401 aattccttct actttgctag ccatcgcctt gtgtaccctc cttagaaatt agaaattcga
     2461 attttacgaa aatgtcttta agccttttcc ggctccggcg ccgtctgggt tccggtcgct
     2521 gcctcggacg cgggacctcc tccaaatttg ttagctttca ccgcttcgct aatgaccacc
     2581 gcgagatttc gcggaagcgc ggccagcgcc gtcgccactc gctgcgcagg ggcgttcaac
     2641 atgaacatga tcttgctgat cagttcttcc ttgctcggaa gctgtgcgag ctggctgatt
     2701 tcctgaatcg aaattacgcg tccttcgacc catcccgcgc ggaacttgaa tgcgggaacg
     2761 tccttggcca ccttgctgag aatcttggct agcgcgacgg ggtccgtctt cgtgtaggca
     2821 atcgaattcg ttccctttaa atccttcagc agtccttcag caggcgtgcc ggcggcagcg
     2881 cgttccgcca ccgtgttctt taccacctcg taatgggcgc ccgcggcttc cactgagcgc
     2941 cgcagcttgg tgtcctgctc caccgtaatt ccctgaaacg tggaaagaat tacggttgaa
     3001 accgtcgcga gttgcgcgcg aagctcttcg ccctgagctt tcttttcggc cttcttcctc
     3061 atgtcgccga gctctccctc ttccctatcc ggttatgctg ctaccgccgt ggcagactcc
     3121 gcctcggtgt ggtcgatcga aacccctgga cccatcgtcg atgtcagcgt gattttcttg
     3181 atgtattttc cttttgccgc cgaaggtttt gctttgacca cagcgcccag caacgcctgc
     3241 gcattttcgg ctaaattctt cgctggaaaa ctgatcttgc ccaccggtga atgaatcagt
     3301 ccggtcttgt ccaaacgaaa ttccacctta ccggccttaa tttcctttac cgccttcgcg
     3361 acctcgaacg tcaccgtgcc cgtcttcgga ttcggcatca atccgcgcgg gccaagcact
     3421 ttgccaaggc gtcccgcgga cttcatcatg tccggcgtgg caatcaccgc ctcgtagtcc
     3481 gtccagcctt cagtgatctt ttgaaccatg tcctcgccgc cgacgaaatc tgcgccggct
     3541 tcttccgcct cgcgcacttt ctcgccgctc gcgatcacca gcacgcgctt ggatttgccg
     3601 agcccgtgtg gcaaaaccac ggtgccgcgt accatctgat cagactgctt cgggtccacg
     3661 ccgagattca aaaccagttc aaccgtttcg tcgaatttca cgaagtgcgc cttgcgaaga
     3721 agttccgcgg cctcttcgag cttatacggg cgcgcttcca ccagttttct cgcgcgatcg
     3781 atattctttc cacctacttt tgcgccgcgg cccatcgttt tcctctctta ttcggcgact
     3841 tcaatgccca tgttgcgcgc cgtgcccatc accgaccgca tcgcggactc gatatccgcc
     3901 gtgttcaagt ccggcatctt ggtcttggcg atttcctcga cctgcttgcg cgtcactttg
     3961 cccaccttgt tacggttcgg ttccggagaa cccttcacaa ttcctgccgc gcgtttcaga
     4021 agcacggacg cgggcggcgt cttcaaaata aatgtaaaag tgcggtcgct atagatcgtc
     4081 accagcaccg ggaaaatcat tccgtccggc tctttcgaag tctttgcatt gaactgcttg
     4141 caaaaatcca tgatgttcac gccgtgcgga ccaagcgctg tgcccacggg cggcgctggc
     4201 gtcgctttcg cggccggcaa ctgcaacttc accattgctt gaactttctt cgccatctct
     4261 cgccctcaat cccttaccag tgtcgtcatt ctgagcgaag cgtcccgcgc ctctggtttt
     4321 gcgcggagcg tcgggacgcg cagtcgaaga atctctcttc gctgcgccag taacctcgct
     4381 tacgcgacct tctccacctg cgcgaaatcc agctccaccg gtgttgaccg cccgaaaatc
     4441 gttaccgaaa ccttcagcgt gctgcgatcc gtattcactt cctcgaccac gccattgaaa
     4501 ttcgcaaacg cgccatccgc aatgcgaatt ttttccccgc gctcaaacga cagcttcggc
     4561 ttgggcttct ctgccgtggt cgttgcgcga ttcaaaatgt tctgtacttc ttcttcggtg
     4621 agcggcgacg gcatttgccc cgatccaacg aagcctgtca ccttcggcgt cgaccgcacc
     4681 acgtgccacg tgtagtcgtc catatccatc tcgactagca cgtagcccgg gtaacacatc
     4741 cgcggcgaaa ccactttctt cccgccgcgc acttccacaa ccgactcggt ggggataacc
     4801 acttggccga tcttgtccgt caagccgaac gcctgtacgc ggccttcgag gctttccttc
     4861 accttcttct cgaagccaga atacgtgtgg acgatgtacc actgctttga cattacgttc
     4921 atctcagtga tggtgaaaat agctgttcac cagaatatac aagcggccaa aaattccgtc
     4981 tgtgatccag aaaaacacgc caaaaaaagc cacggtaatc gtcaccacaa ccgtcgtgga
     5041 ccagacgtca gaacgcgacg gccagttcac ttgcttcatt tccacgcgta catcgtgcag
     5101 aaaatttcgc aggcgtttgg gatagccgcc cagtttctgc gccggctcca tcagcgaaaa
     5161 cccgcttacg cgatggcttt cctcgtcccg gtttgtccgt gctgcatctg ccatctgctc
     5221 gcctgtgctc tccctcgcgt ctaactaccc gccagcttcg ctgcccccaa atactggcag
     5281 gggaggaggg attcgaaccc ccactctcgg ttttggagac cgatggtcta ccgttgaacc
     5341 tactcccctt taccctgaga acccgccgtt cgcgggtttg aagggtttac ccgcagttcg
     5401 caggctcgaa gggtttaccc tgcccattcg cagggctacg ggttgccctt caccattctt
     5461 ttcgataaga gccaacttcc tactccggct ccagcctttt atttctttct ctcgggcccg
     5521 cgcgaccttt gagccgtcgc agcgctcact ccaaaccaat ccgactggcc tgcgtttcgc
     5581 agtaaaccct gggccaactc cccagttgtg tcgtttcact cgttcggcaa catcccttgc
     5641 gatgcctaca tacaatgatc cgtcattgca tcgcaccatg tagcagaacc aaggctcatc
     5701 tccgtctgcc atcgagccag ccttacttca cttccttgtg ccccgtatgc ttccggcacg
     5761 tattgcaaaa cttctgcgtt tccagccgct cagtatgctt cttgcggttt ttggtggtcg
     5821 tgtagtttcg gtttttgcaa tcgttacact gcaaagtgat gatctctcgc atggtccttt
     5881 actcgacgat ctcggcaacg gcgccggcgc ctactgtgtg gccgccttcg cgaatcgcga
     5941 accgcaatcc cttctccagc gccaccggcg tgatcaacgt gatttccgcg ctcacattgt
     6001 ctcccggcat caccatttcc actcccgtcg gcagcttcac gtctcccgtc acg
//
LOCUS       DATNDT010000037         7211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3660802, whole genome shotgun sequence.
ACCESSION   DATNDT010000037 DATNDT010000000
VERSION     DATNDT010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7211
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3660802"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..301)
                     /locus_tag="VN788_01295"
     CDS             complement(<1..301)
                     /locus_tag="VN788_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXU19196.1"
                     /translation="MPIVMDNTLNQIRSTAASGGDRVGRAKRLAEMIRKLGEYRWVGV
                     YDVGVENVSIVAWSGPSAPEHPTFPVSKGLTGAAITQKKTIISGDVRNDPRYLTAF"
     gene            complement(323..1708)
                     /gene="ffh"
                     /locus_tag="VN788_01300"
     CDS             complement(323..1708)
                     /gene="ffh"
                     /locus_tag="VN788_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HXU19197.1"
                     /translation="MFETLTEKLQRAFKNLRGQGKLTEEHLDAALAEIREALLEGDVN
                     LGVADDFIAQVRQKALGSEVLLQLSPDQQVVKIVRDELAELLGREARPQFSSRPPSVW
                     LICGLQGSGKTTSTGKLGKWLAEHGHRPIVVSTDVYRPAAREQLAQVAKATSTAIWPG
                     TGTDKPLEIVRGATREAKLSACDVILVDTAGRLHIDDELMNELSMLKKELSPAEILFV
                     ADAMIGQDAVRSAGEFHKRLGVTGVILTKMDGDARGGAALSIRKVTGAAVKFVGVGEK
                     YDALEPFLPDRVVSRILGMGDVLGLIEKAQKNVDEKKAREMERKLRENDFTLADFRDQ
                     LQQIRKMGSMEQLIGMLPKVGPFAKIPKDASIDEKQLKSTEAIINSMTAAERADDNII
                     NGKRRKRIARGSGTTVQEVNQVLKQYSDMRRMMKMYGNTMRGKMRGLSKLRGMGGLGG
                     VGGAGGAGAAE"
     gene            complement(1715..2602)
                     /locus_tag="VN788_01305"
     CDS             complement(1715..2602)
                     /locus_tag="VN788_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HXU19198.1"
                     /translation="MAISQTERARKFRALHKLPATSGAPIESSGPGAFIIPNPWDAGS
                     ARILAGLGFEALATTSAGFAFSLGRRDGAVTREEALAHCRAIANATDLPVAADLENCF
                     GNDPKTVAETIRLAGSTGLVGGSVEDSSGDDHRPIYDYALAVERVAAAVEAARALPFP
                     FTLTARAENFVHGRSDLDDTIRRLQAFAEAGADVLYAPGLPNLETIKAVCSAVAPHPV
                     NGLAGMKGRAFSVADLTAAGVRRISLGGVLSRVAYAALISAAQEMQQRGTFTFVDHVP
                     PNADIAGFMAPKKTPAGTA"
     gene            3118..3306
                     /locus_tag="VN788_01310"
     CDS             3118..3306
                     /locus_tag="VN788_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19199.1"
                     /translation="MQLAIAPRKEQPYYPRMIVKTLIRVCREELLKAKRAIVFAYGAI
                     READERTMDLLMMFLGIR"
     gene            complement(3426..4985)
                     /locus_tag="VN788_01315"
     CDS             complement(3426..4985)
                     /locus_tag="VN788_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19200.1"
                     /translation="MIALCRVIVVLLSALTVSLPAALCQEEHQHPVPEKLGEAFFPVS
                     CAPAVQEPFNRGVAVLHSFAYSAAEKIFLDITRADPKCAMAHWGAAISYYHQLWEPPI
                     TPQDLQRGQLEIAKAAALGGRSPREQGFIDAAALFYKDAPRVPHSTRALAYQKAMAAV
                     AARNPNDREAQIFYALALLSTAPSTDRNHMNQKRAAGILEPLFKEYPEHPGVAHYLIH
                     AYDNPELAKRGLPAARAYAQIAPSAPHALHMPSHIFTQLGLWQDSILSNIAARAAAHH
                     QKDIGEELHSMDFLMYAYLQAGRNSEASQLLEELRTMPGLPVSQFKVGYAATAMPVRY
                     AVERREWAEAAANSPKPGSAPEVLATTYWCRALGLARSGKPNAAATEVEKLDQSLRQV
                     REKNDAYWAAQVEIQLEEAKAWIAYASGRKNISVSLLRTSAEKEESLEKRPVTPGPII
                     PAREQLADLLLELNRPVEALREFEVSLANAPRRRGGLSGATRAAELAGDSTKADHFKK
                     QLLALGSATTN"
     gene            5452..6354
                     /locus_tag="VN788_01320"
     CDS             5452..6354
                     /locus_tag="VN788_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007938387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HXU19201.1"
                     /translation="MYVITGASGNCGHVIAEKLLDAGKKVRAIGRSAERLQHLRARGA
                     ELFVCDLSERTALAKGFEGAEGVFVMIPPSAASADYRKEQDGITNSVTAAIEDAGVKH
                     AMSLSSFGADKAKGTGPVAGLHNLEKRLNAISGLNVLHLRAGYFMENTLPQIGIIKAL
                     GTAAGPLRAGVALPMIATRDIGAYAAQALIGLRFSGQQTHELHGQRDITMAEAAAIMG
                     TEIGRPGLAYKQLPNDLVRKALTDTGMSQNVADLILEMAEAINSGHMATLEKRSPENT
                     TPTSFETFTKEVFAPQFKGKSTAA"
     gene            6639..>7211
                     /locus_tag="VN788_01325"
     CDS             6639..>7211
                     /locus_tag="VN788_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19202.1"
                     /translation="MATIPGPLRSFLRMAAISQKVSPDEVIPLLSRNVFLTGYTGAGR
                     KGQPTEFLLLLRQYVQQARELSVLAGPTGVIHVSNCNDARLLLQVLGYSAGRDCGQRT
                     TFLKTEDAQRAFLTTDSGFPLSELERTLSGGAPFNYPYPSSKVPILLTEQDWTAASEH
                     GGKGENGRDLLDALLHDPVLAHLYYAWAHID"
BASE COUNT         1480 a         1986 c         2217 g         1528 t
ORIGIN      
        1 caaacgctgt caggtatcgc ggatcgtttc gaacgtcgcc cgagattatc gttttctttt
       61 gcgtgatcgc cgcgcctgtc agacccttgc tcaccgggaa tgtgggatgt tccggtgcgc
      121 tggggccgct ccaggcgacg attgagacat tttccacgcc cacatcgtaa acgccgaccc
      181 agcgatattc accaagtttg cgaatcatct ctgcaaggcg cttggctcgt cccacgcgat
      241 cgccgccact ggccgccgtg gagcggatct gattcagcgt gttatccatc acaatcggca
      301 tcgtcgtttt atccttggct catcattccg ccgcgccggc tccgccggct ccgccgactc
      361 cgcctagtcc gcccattccg cgtagcttac tgaggccgcg cattttcccg cgcatggtgt
      421 tgccgtacat tttcatcatg cggcgcatgt ctgaatattg tttgaggacc tggttcactt
      481 cctggacggt ggtgccgctg ccgcgggcga tgcgcttgcg acgcttgccg ttgatgatgt
      541 tgtcgtcggc gcgctcggcg gcggtcatcg agttgatgat ggcttcggtg gatttgagct
      601 gcttttcgtc gatggaagca tctttcggaa ttttagcgaa ggggccgacc ttcggaagca
      661 tgccgatcaa ctgttccatc gagcccattt tgcgaatttg ctgaagctga tcgcggaaat
      721 cggcgagggt gaaatcgttt tcacggagct tgcgctccat ctcgcgcgct tttttctcgt
      781 caacgttctt ttgggctttt tcgatcaggc cgaggacgtc gcccatgccc aggatgcgtg
      841 agacgacgcg gtcgggaagg aacggttcca aggcgtcata tttttcgccg acgcccacaa
      901 atttcaccgc cgcgcccgta actttgcgga tggaaagggc cgcaccgccg cgtgcatcgc
      961 catccatttt ggtaaggatc acgccggtga cgccgaggcg cttgtgaaat tcacctgccg
     1021 aacgcacggc gtcttgtccg atcatggcgt cggcgacgaa cagaatttcc gcgggagaca
     1081 gctctttttt caacatcgag agttcgttca tcaactcgtc gtcaatgtgg aggcggccgg
     1141 cagtgtccac caaaatcaca tcgcaggcgg aaagttttgc ttcgcgggtg gcgccacgca
     1201 cgatttcgag cggtttgtcg gttccggtgc caggccagat agcagtgctc gtggcttttg
     1261 caacttgcgc gagctgttcg cgcgccgcgg ggcgatacac gtcggttgaa acgacgatgg
     1321 gccgatggcc gtgctcggcg agccattttc cgagcttgcc ggtagaagtg gttttgccgg
     1381 agccttgcaa tccgcagatc agccataccg agggcggccg cgaagaaaat tgcggacgcg
     1441 cctcgcggcc gaggagttcg gcgagttcgt cacgcacgat tttgacgact tgctgatcgg
     1501 gcgaaagctg gagcaggact tcggagccga gagctttctg gcgaacctgg gcgatgaaat
     1561 cgtcggcgac accaaggttc acgtcgcctt cgagcagcgc ttcgcgaatt tcggcgaggg
     1621 ccgcgtcgag gtgctcttcg gtgagcttgc cctggccgcg aagatttttg aaagcgcgct
     1681 gaagtttttc ggtgagcgtt tcgaacattt tttcttacgc ggtcccggcc ggagttttct
     1741 ttggcgccat gaagccggca atatctgcgt ttggcggcac gtgatccaca aaggtaaagg
     1801 ttccccgttg ttgcatttct tgcgccgcac tgatcagggc ggcatatgcc acgcgggata
     1861 atacgccgcc caaactgatt cgccgcacgc ccgcggcggt aagatccgct acggaaaacg
     1921 cccgcccttt cattccggcg agaccgttca ccggatgcgg cgcgacagcg gagcacaccg
     1981 ccttgattgt ttcgaggttc ggcagaccgg gcgcgtagag gacgtcggcg ccagcctccg
     2041 caaatgcctg aagacggcga atcgtgtcgt cgaggtccga gcgaccgtgg acgaaatttt
     2101 ccgcgcgggc tgtcagcgtg aaaggaaacg gcaaagcccg cgcagcctcg actgccgccg
     2161 caacacgctc gacggcgagg gcgtaatcgt aaatcgggcg gtgatcgtca ccgctcgaat
     2221 cttcgacgga accgccaacg agtcccgtgg agcccgcaag gcgaatcgtt tctgcgacgg
     2281 tcttcggatc gttgccaaaa caattttcga gatcggcggc gacgggcaaa tcggtggcat
     2341 tggcgatcgc ccggcagtga gcaagtgcct cttcgcgggt gaccgctccg tcccggcggc
     2401 cgagcgagaa ggcaaagcct gcgcttgtgg tggcgagggc ctcgaaaccc agtccagcca
     2461 gaatgcgcgc actgcccgca tcccacgggt tggggataat aaacgcgcct gggccggacg
     2521 attcaatcgg cgcccccgac gtggcgggga gcttgtgaag agcccggaat tttcttgctc
     2581 tctctgtttg gctgatagcc atggcagcct ttatcgctgc tgcgccccag agcgcggagc
     2641 gccagcgcgg taactcccga ttttaggcga ggcgcggagt gcggtcaaat gcgatggttt
     2701 gagaagcagc cagccagtag ggttctgcga gcgtgacggg tagaaatggc cgcggtcttt
     2761 tgttttcttc gggttgcgat atattctact cggctcgagt gtcgattaga aagtgacggg
     2821 agattcctcg gccggttaaa tttcggaggc gtcgcccctc gggacaaccc ttcgtcggtg
     2881 gggaaatggt agctaaggat gtttgccagg cacgatgtat cgcgccccaa gggcaaatcc
     2941 ccacggtggg gaatgcgcgc atcgtagcta ctgtgtcggt ctctcatgcg gtgtcctttc
     3001 cgggccgagc gcgccctgat tcacgctacc actcggacgg ttcgattttg tcaagaaggc
     3061 ctgtggactt tatttttcgt agacaggaga gcgtgcagtt attagagcgc gacattagtg
     3121 caattggcta ttgcgccgag gaaagagcaa ccgtattatc cgcgcatgat cgttaaaaca
     3181 ttaatccggg tgtgccgcga agaattgctg aaggcaaagc gcgcgattgt attcgcgtat
     3241 ggtgcgatac gcgaggccga cgagcgcacg atggacttgc tgatgatgtt cctcgggatc
     3301 cgctgaaata cccgcaataa gcggcggcgc tcacatcatt caaatccttg gcagcaagct
     3361 tccgctgagc ttattcaatc gagaatatgc aatctcgtgc gattttgcgc acaacaattt
     3421 gcgattcagt ttgttgtggc agagcccagc gcgaggagct gcttcttgaa gtgatcggcc
     3481 ttcgtcgaat ctccagccaa ttccgcagcg cgagtcgcac cagataaacc tccacgacgt
     3541 cgaggcgcgt tcgcgagaga gacttcaaac tcccggagag cctcgacggg ccggttcagt
     3601 tctaagagca aatcggcgag ttgctcgcgc gccggaatga tgggacctgg cgtaacagga
     3661 cgcttctcca agctttcttc cttttcagcg gaggtgcgca acagggacac ggaaatgttc
     3721 ttccggccgc tggcatacgc aatccaagcc ttggcttctt ccaattgaat ttccacctgc
     3781 gcggcccagt aagcgtcgtt tttctcgcga acttgccgga gggattggtc cagcttctca
     3841 acctctgtcg ccgcggcatt cggcttaccg ctgcgcgcca ggccaagcgc gcgacaccag
     3901 tacgtggtcg ccagcacttc cggtgcactc cccggcttcg gtgaattcgc cgccgcttcc
     3961 gcccattcac ggcgctcgac ggcataacga acaggcatcg cggtggcggc atagccaacc
     4021 ttgaactggc tgaccggaag acccggcatc gtccgtaact cttcgagaag ttgcgaggct
     4081 tcgctgttgc gcccggcctg aagatacgcg tacatcaaga aatccatgga atgaagctct
     4141 tcgccaatat ctttttggtg atgagcggca gcgcgcgcgg cgatattgga aaggatagag
     4201 tcttgccaaa gcccgagctg agtgaagatg tgggaaggca tgtgcagggc gtgcggagcg
     4261 gagggggcga tttgcgcgta ggcgcgggcc gcgggcaatc cacgttttgc gagttcagga
     4321 ttgtcatatg cgtgaatcaa ataatgagcg acacccgggt gctcgggata ctccttgaac
     4381 aggggttcga gaattcccgc tgcgcgtttc tgattcatgt ggttccgatc ggtggagggc
     4441 gcagtcgaga gcagggcgag tgcgtagaag atctgggctt cgcggtcatt cgggttgcga
     4501 gccgcgacgg cagccatggc tttttgatag gcgagcgctc ttgtcgaatg agggacgcga
     4561 ggcgcatctt tgtaaaacag cgcggcggcg tcaataaaac cctgttcacg cggagaacgg
     4621 ccgccgaggg ccgccgcttt cgcgatttcc aattgtccgc gttgcaagtc ttggggcgta
     4681 atcggcggtt cccacaactg atggtaatag gagattgccg cgccccagtg ggccatggcg
     4741 cacttcggat cggcccgcgt gatatcgaga aatatttttt ccgccgcaga ataggcaaag
     4801 gaatgcaaga cagccacacc ccgattgaag ggctcctgga cagcgggggc gcaagatacg
     4861 ggaaagaatg cttcaccgag tttttctgga acaggatgct ggtgctcttc ttggcacaac
     4921 gcggccggca gggatacggt gagtgcggat aacaaaacga cgatgacccg gcaaagagcg
     4981 atcatcctga actccagaga agagcggcat caaaccgaac tgcggcattt gccgactgga
     5041 ctgcgcacag agcgtatcac ttttgtgggc acgggaaaat gaacctggcg ctcgcggccg
     5101 ctgagttctc cgtttatggc ggcttgctgc tgaacgttca tctgcggttg acttgatttc
     5161 aacgagcaat gggtgaagaa tgggcggcga ggttaaaacc ttcgcccttt cgaagcgttg
     5221 actgaaacat tcaccgcaac gtccccagca cgctgttaat ccaatacctg atcccaaagg
     5281 atcacagatc ggtgtagtga ctgagtttgc tggtggcaac agcgactttt ggctgggact
     5341 tgccgattgc ctcaacttac gcccgcaggc aaaagtgcct gcaccacgaa agaattgact
     5401 ttcgcgaacg ccaaaaagat ccattaaaaa acaaagaaag agaggaactc tatgtatgtg
     5461 attacgggag caagcggaaa ttgcgggcat gtgattgcgg agaagctact cgatgcagga
     5521 aagaaagtcc gggcgatcgg gaggagcgcg gagcggctgc agcacttgcg ggcccgcgga
     5581 gcggagcttt tcgtgtgcga tttgagcgaa cgaacagcac tggcgaaagg cttcgagggc
     5641 gcggaaggtg tcttcgtgat gattcccccg agtgccgcga gcgcagacta tcgcaaggag
     5701 caggatggta ttaccaattc cgtgacggcg gcgatcgaag atgcgggagt gaagcacgcg
     5761 atgtccctga gcagtttcgg agcggacaag gccaagggca caggccccgt ggcgggattg
     5821 cacaatctgg agaaaaggct caatgcgata tccggtctga atgtgctgca cttgcgcgcc
     5881 ggatatttca tggagaacac gctgccacag attggaatca tcaaggcttt ggggacagcg
     5941 gcgggcccgc tgcgggcggg agttgcgctg ccgatgattg ccacgcgtga catcggcgct
     6001 tatgcggcgc aagcgctgat tggacttcga ttcagcggac agcagacaca tgaacttcac
     6061 gggcagcggg atatcactat ggccgaagca gcggccatca tgggcacgga gatcggcagg
     6121 cccggtctcg catacaagca attgccaaac gaccttgtgc ggaaggcatt gacggataca
     6181 ggaatgtcgc agaacgtggc ggacctgatt ctggaaatgg ccgaggcgat caactccggg
     6241 cacatggcga cgctcgagaa acgctcgccg gagaatacca cgccgacttc gttcgaaact
     6301 ttcacgaagg aagtgttcgc gccacaattc aaggggaagt caaccgcggc ataatttgac
     6361 gacggcctgt ccgccggtgt caaccgcaac tagcggttga caccggcggg gaggagggct
     6421 aggctttcgg cgcgaacgag tctcatggct ccggttggtg gaactggtgc cagacattgc
     6481 aatgtaggct ggcaatagcg atgaaacacc cccgttacct ttcagcccct tttgcggttc
     6541 tagtgtgcat ctgcgccggc atggtcccca gcggttgggg cgcgacttcc cctccttccc
     6601 cggtttctca aggaagtgtg gtcgagaccc aaagctcaat ggctactatc ccgggaccct
     6661 tgcgctcctt tttgcggatg gcggcgatca gccaaaaggt ttcccccgac gaagtgattc
     6721 cgctgctttc tcgcaatgtg tttctcaccg gctacacggg tgcgggtcgc aagggtcagc
     6781 cgaccgaatt cctgctgctg ctgagacagt acgtccaaca ggcgagggaa ctgtcagttt
     6841 tggccggtcc cacaggcgtg attcatgtgt cgaactgcaa cgacgcgaga ctattgctgc
     6901 aagtcctcgg ctacagtgcc gggagggatt gcgggcaacg cacaactttc ctcaagacag
     6961 aagatgcgca aagggcattt ctcacgacgg attcaggctt ccctctctct gaacttgaga
     7021 gaacgctatc cgggggagct ccgttcaact atccgtaccc aagctctaaa gtacccatat
     7081 tgctaacgga gcaggactgg acggcggcaa gcgagcatgg tggcaaaggc gaaaacggcc
     7141 gagacttgct tgatgcgctt ttgcacgatc ctgtcctggc acacctttac tacgcctggg
     7201 ctcacataga c
//
LOCUS       DATNDT010000038         3264 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_501286, whole genome shotgun sequence.
ACCESSION   DATNDT010000038 DATNDT010000000
VERSION     DATNDT010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3264
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_501286"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(653..2260)
                     /locus_tag="VN788_01330"
     CDS             complement(653..2260)
                     /locus_tag="VN788_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HXU19203.1"
                     /translation="MGIHGIELIVLLLMILVVAFASVAKRLETPYPIILVIGGLLLSL
                     FPRGPHIELDPDIVFLLILPPLLFSAAFVTSWRDFRYNLVSIVMLAFGLVGFTVFGVA
                     AASRWILPGFDWRMGLVLGAVVSTTDAVAATSIGRRLGLAKRIVDILEAESLVNDASG
                     LLALEFATALLVTGHTPSVGEGLWRLLYLVGASIVIGLVIGKLIHFVSLKVEDAPIEI
                     TISLIAPYFAYLAAEGARTSGVLATVVCGLYLGHKSSFYLSTGARLTGQAVWDTLTFV
                     LNGFVFLLIGFQLPYILSGISTHHLGGLVLLGALFSGVVIVLRLIWIYPGAWLSNFIR
                     RRLLRQPEPFPSSRSVFIIGWTGMRGVVALAAAISLPKLLESGALFPERNMMIFLTFC
                     VIFVTLVLQGLTLPPLIRRLGLASAGDGKIEEEAARRAMVEATLAYLEHSRDDHPEFA
                     PLHDELTRSYRRRLNAIDKNASAEAGYRPEDYERWKALSRQLGAIQRATILHLRNQNE
                     INDEVARSLERELDLAEARYLLAERPQ"
     gene            complement(2393..3184)
                     /locus_tag="VN788_01335"
     CDS             complement(2393..3184)
                     /locus_tag="VN788_01335"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HXU19204.1"
                     /translation="MNRRELLKKSAALGFMAAAPAVIVGEGFAGRGGAATSKGAYQFS
                     SALSASLKPPKSGIISVAYVLSHGVVEVDFTGPWGIFGSVMLPGKDMVMPFHQYTVAE
                     SKSPLRTDSGLAIVPDYTFDTAPGPKIIVVPAQGRVPEGMVKWIRKASAGTDVTMSIC
                     TGAFVLAKTGLLDGKSATTHHSAYTRFADEFPKTHLVRGVRFVDEGNVATAGGLTCGS
                     DLAMHVVERYFGRKLAEDTAYYLEYQSLGWKDPSSNAIYARPSKS"
BASE COUNT          753 a          970 c          891 g          650 t
ORIGIN      
        1 ttttgcgcgg ttcttaggca ttgctatttt agaattttcc ccttttttta agagcgaagg
       61 aatataaaac ggcgcgatgt tacctgaagg gcaacacaat gtccagcgcg gcattgcgta
      121 aaaaagtacc atctcggcaa gacaggctcc cgttctttca aggaatcagg gagtttcgtc
      181 ggccgcaaga gcgcccgctc gagcaaaacg tcgattgctc gatcgagccc gcgatgcctt
      241 gcctcgttag atttagcgga atcaaaccac tcgatgaaat gacggcgccc ccccggcgtg
      301 agtgcttcga atgccttcca cgccttgggg tgcagcttca gacccgcacg aagatcggga
      361 gggaccggca gagttctcga agagagatcg agccgaagct cgatgccgac tacatcgccg
      421 acgtccgcct gccggcacga gtgtcgcccg cgtgatctca ccgtttacga ccgctacgac
      481 gggaatatat ttcggctcgg cggattttgc gggcttgcgc tgtttggtct ttccccgcga
      541 aacttcttca ccagcccgcg tgccacatcg tcgggcacat ccacgtgccg catcatccaa
      601 attttgtaga tcgtcgccat gaatcgctgc ggtgctggcc gccggacagt cttcactgcg
      661 gccgctccgc taacaagtac ctggcctcgg ccagatcgag ttcgcgttcg agcgaacgcg
      721 ccacttcgtc attgatctca ttctgatttc gcaaatgcag aatcgtggcg cgctggatgg
      781 cgcccagctg ccgggaaaga gctttccagc gctcgtaatc ttcgggccgg tagcccgcct
      841 ctgcggaggc attcttatcg atggcgttca gccggcggcg gtaggaccgg gtaagttcgt
      901 cgtgcagcgg agcgaattcc ggatggtcat cgcggctgtg ttcgagatag gccagcgtgg
      961 cttccaccat ggcgcgccgc gccgcttctt cttcaatctt cccgtctccc gcgcttgcca
     1021 atcccaggcg gcgaatcagc ggcggcagcg tgaggccctg caaaacgaga gtgacaaaga
     1081 tcacgcaaaa agtgagaaaa atcatcatat tgcgttcggg aaagagagct ccgctctcca
     1141 aaagcttcgg cagggaaatg gccgccgcca gcgccaccac gccgcgcatg cctgtccagc
     1201 ctatgatgaa gacagacctc gaagacggaa acggttcggg ctgccgcagc aagcggcgcc
     1261 gaatgaaatt tgagagccac gcgccgggat aaatccaaat aagccgaagg acaatcacga
     1321 cgccgctgaa tagcgcgccc agaagcacca atccgcccaa atggtgagtg ctaatgcccg
     1381 aaaggatata aggcagttga aatccgatca gcaggaaaac gaatccattg aggacgaacg
     1441 tgagtgtgtc ccacaccgcc tgccccgtta atcgtgcgcc tgttgataaa taaaaggagc
     1501 tcttgtggcc aagatagagt ccgcacacga cggtggcgag cactcccgaa gtgcgggcgc
     1561 cttccgcggc gagataggcg aaatacggcg caatcagact gatggtgatt tcaatcggag
     1621 cgtcttcaac cttcaatgat acgaagtgaa tcagcttgcc gattaccagc ccgatcacaa
     1681 tgctcgcgcc gacgagatag agcagcctcc acaggccttc tccgacagaa ggcgtgtgcc
     1741 ccgttaccaa tagggcggtc gcaaattcca gagccaataa gccgctcgca tcattgacca
     1801 gactttccgc ttcgagaata tccacgattc tcttcgcaag accgagacgc cggccgatgg
     1861 aagtggcggc aaccgcgtcc gtggtcgaaa cgaccgcgcc cagcacgaga cccattcgcc
     1921 agtcaaaccc cggcagaatc cagcgcgatg ccgcagccac gccgaacacc gtgaacccga
     1981 ccaggccaaa ggccagcatc actatgctga cgaggttgta gcggaagtcc ctccaagagg
     2041 tcacaaaggc cgcggaaaac aacagcggcg gcaaaatcag cagaaacaca atgtcaggat
     2101 caagctcaat gtgcgggccg cggggaaaca gactgagcag caatccgcct atcaccagaa
     2161 tgattgggta aggagtttcg aggcgctttg caaccgacgc aaacgccacg acgagaatca
     2221 tcagcagaag aacaatcagc tctatgccat gaatccccac tttgcctgac ccctcgcagg
     2281 caaagatacc gtaccgaacc ggaccctgca cctttttcca ccgtcagcga aaaaaggccg
     2341 cactcgcttt ttggcggatg cggcctttca aacctggtcg gtttggggct tatcagctct
     2401 tagacggccg ggcatagatc gcgttcgagc tagggtcttt ccatccgagg ctctgatatt
     2461 cgaggtagta agcggtgtct tccgccaact tccttccaaa atatctctcg accacgtgca
     2521 tcgcaaggtc gctaccacag gttaggccgc ccgcggtcgc aacgttgcct tcgtcaacga
     2581 atcggacgcc gcgcacgaga tgggttttag ggaattcgtc cgcgaaacgt gtgtaagcgc
     2641 tgtgatgagt ggtcgctgac ttcccgtcga gcagcccggt tttcgccagc acgaacgcgc
     2701 ccgtacaaat cgacatagtt acgtccgtgc cggccgatgc cttgcgaatc catttcacca
     2761 tcccttcagg aaccctgccc tgcgccggaa ctacgataat ttttgggccc ggggcagtgt
     2821 caaatgtgta gtcaggtacg atggccaaac ccgaatcggt gcgcagtgga gacttgcttt
     2881 ccgcgacggt atattgatga aagggcatca ccatatcctt gccaggtagc attactgagc
     2941 cgaaaatgcc ccaaggtccc gtgaagtcaa cttcaacaac gccgtgcgaa agcacatatg
     3001 cgaccgagat gattccgctt ttgggaggct tgaggctcgc gctgagtgcc gagctgaatt
     3061 ggtacgcgcc tttgctcgtc gccgctccac cgcgcccagc gaatccttcg cctacaatta
     3121 cggccggcgc agcagccatg aatcccagcg cggccgattt cttgagtagc tctcttcggt
     3181 tcataccaac ctcctctttt gacttttgga acttcgagtt tgtcgcagcg aaacgttcga
     3241 gctagtccta gttaagtccg tgaa
//
LOCUS       DATNDT010000039         5016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1666197, whole genome shotgun sequence.
ACCESSION   DATNDT010000039 DATNDT010000000
VERSION     DATNDT010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5016
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1666197"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(396..1400)
                     /locus_tag="VN788_01340"
     CDS             complement(396..1400)
                     /locus_tag="VN788_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase"
                     /protein_id="HXU19205.1"
                     /translation="MRKKIRVVQFGVGPIGASIVRLMREKAALEIVGAIDRDPAKAGR
                     DLGELIGANDAPWGVTVNAEASAALAHPVDVIVHTTSSYLTNVMDELLACLSAGCCVV
                     STCEELAYPFRKHTELSQKLDTAAKNEGVALVGTGVNPGFVMDKLVLTLAAASQRVDW
                     AKAVRVVDASKRRLPLQKKIGAGMTPDEFRAQVAAGVIKHHGLPESIAMVADGLGFAL
                     DDISETIEPITAKETVKTEFLEVAPGQVAGVHQIARGTTAGKEKIFMELQMYVGAKHP
                     ADSVELRGEPNLALTIPGGTHGDIATAAVAVNAIPAILAAPAGLRTVRDLPLTFFPPT
                     "
     gene            complement(1721..2590)
                     /gene="bla"
                     /locus_tag="VN788_01345"
     CDS             complement(1721..2590)
                     /gene="bla"
                     /locus_tag="VN788_01345"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HXU19206.1"
                     /translation="MCALRWALALAIVMAFPAFVRAQANDLSRNMNQPVPPFHIIGNI
                     YYVGASDITSYLIVTSAGDILLDGGFVETAPQIEANIKTLGFKLADVKFLISSHEHLD
                     HAGGFAELKRSTGAQLVAMAEEVPTLTSGKSFPAVTPDRVIHDGDTVSLGGVTMTAHL
                     TPGHTRGCTTWSMIARDNGKSYNVVFVGSASVLPNYKLIDRPNTPATYSGIQADYEKT
                     FRVLRSLPCDVFLGAHGSFYSLKQKREAMEKHPAANPFIDPAGYQAYIARAEAVYRTE
                     LQRETAPASTAAH"
     gene            complement(2623..3576)
                     /gene="bla"
                     /locus_tag="VN788_01350"
     CDS             complement(2623..3576)
                     /gene="bla"
                     /locus_tag="VN788_01350"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: protein motif:HMM:NF033105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="subclass B3 metallo-beta-lactamase"
                     /protein_id="HXU19207.1"
                     /translation="MTSNRLSARRIGLSSLALATATLICGAAIAQAPAGSPPQGQTAA
                     GGAAAAAAAMKNPFPPFHMIGNIYYVGSAGLSCYLINTPRGNILLDTGYPDMAPQIEG
                     NIEKLGFKLKDTKLLINTHAHIDHGGGMAAIKAATGGKLVAMAEDAPFFENGGHNDVL
                     FGDRNLFPAVKVDRVVHDGDQVKLGGVTLTAHRTPGHTPGNTTWTMVTTDKDGKSYNV
                     VFFGNVTLSPNTNLKGTAAYPSMGADWAKTMQVLPMLKCDVFLGSNSSFYDMEKKHQE
                     LTSDPNAPNPFIDPTGCKAFVDRGEATYQKLLASPTQAEAR"
     gene            3742..4800
                     /locus_tag="VN788_01355"
     CDS             3742..4800
                     /locus_tag="VN788_01355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HXU19208.1"
                     /translation="MKNALLAVIAALCVFLYTGTGTKAQDQAAPPPPGAPTTAAAAPG
                     ATAPQGRKKRIAVFDFDYATVQTASSAAFGTNVDVGKGITDLLVKYLVQDGTYSVIER
                     QALDKIMAEQNFSNSDRANPTSAAKLGKLLGVDAIIVGAVTQFGNDAKNQNIGGGGGN
                     WGGFGVGGFSHKKTKAIVNIDARIVDIDTAEILGVAEGHGESSRSSTGLLGGGGNWHG
                     WGNGAVDFGSSDFQQTILGEAVKAAVEQMSTGLVADNTKLQTRTIVVSGLVASVDGGQ
                     IVLNVGGKAGLKVGDQLSVERVTKEIKDPATGAVIRRLASPVGVVRVTDVDDISAVAA
                     PVSGAGFKVGDAVKTVTQ"
     gene            complement(4815..>5016)
                     /locus_tag="VN788_01360"
     CDS             complement(4815..>5016)
                     /locus_tag="VN788_01360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19209.1"
                     /translation="ETPHAPWTIFLYTNNYLRALTCPRQNIARILVLRPFGFMWSAAA
                     RRRCLPPGLTRACSSREQRSRF"
BASE COUNT          994 a         1324 c         1653 g         1045 t
ORIGIN      
        1 ccgcggagtt agctttattt cgtcacggtt tcggccggat tgctcccaga agcggtggca
       61 catcgcgcca agcacgttgg ccgggcacag cgtgctggcc cctaggcaga aaaaccctgg
      121 cacgctcatg ctcacggcga gacgcatggc gatcgaagag aggtccttcg gctgcgtttc
      181 gcgcccacaa aaaccgtggg agaaagcaga accgcgcgaa actccgctca ggatgacagc
      241 cctgcagaaa aagccaagac gccactcgga agattggcgt tcccaggaac gacaaggcta
      301 atcacgcgct ccgcagcgcc cgaccgcttg agcacatcac accaagcacg ttcgccgggc
      361 ccagcatgct gtgcccgtac aactgcaaaa gcccgtcagg tgggcgggaa gaaggttagc
      421 ggcaggtcgc ggacggtgcg gaggccggcg ggagcggcga ggattgccgg gatggcgttg
      481 acggctacgg ctgcggtggc gatatcgccg tgcgtgccgc cgggaattgt tagcgcgaga
      541 ttcggctcgc ctcgcagttc gactgaatcc gcggggtgct ttgctcctac gtacatttgc
      601 aattccatga agattttttc tttgccggcg gtcgtgcctc gcgcgatttg gtgaactccg
      661 gcaacctgcc ctggagccac ttcgagaaat tctgtcttca cagtttcctt ggcggtaatt
      721 ggctcgatgg tttcggagat gtcgtcgagc gcgaagccca ggccgtcagc gaccatcgct
      781 atggattctg gcaagccgtg atgcttaatg actccggcgg caacctgggc gcgaaattcg
      841 tcgggagtca tgccagcgcc gatttttttc tgtagcggga gacggcgctt cgatgcatcg
      901 acaacacgga cggctttcgc ccaatccaca cgttgtgagg cggcggccag cgtgaggacg
      961 agtttgtcca tcacgaagcc gggatttacg ccggtgccga ccagggcgac gccttcgttc
     1021 tttgcggccg tgtcgagctt ttgtgaaagt tccgtgtgct tgcgaaatgg ataggccaat
     1081 tcctcgcagg tggagacgac gcagcatccg gcggagaggc aggcgagcaa ttcgtccatt
     1141 acattcgtga gataggagga agttgtgtgg acgatgacat cgactggatg ggcgagagcg
     1201 gcggaggctt ccgcgttaac ggtgactccc cacggtgcgt cgttcgcgcc gatcagttca
     1261 ccgaggtcgc ggccggcttt cgcagggtcg cggtcgattg cgcccacgat ttcgagtgcg
     1321 gccttctcgc gcatcaggcg aacgatggat gcgccgattg gtccgacgcc gaactggact
     1381 acgcggattt tcttcctcac gagagcgtcc tctgggcgaa aaataaacgt ctaaggtaac
     1441 agaattggcg gaattggctc tacaagcagg ttcaatcgtc cctccgggac ttttcgattt
     1501 tcgtttccgg atcccggcac ttcgcttcgc tgcgtgccgg gctaattaca atcgcgcctc
     1561 cggcgctgac agacctttaa ggcgccgcgc ccgcaccgga attcgggatg gcaatgttgt
     1621 gcactggggg cgcacaggca gtactttgtg tttggggcgg ccggcctgat tgccgccagt
     1681 acgacggccg gcctacaaac cccgggtcgc gccaattccg ttagtgcgcg gctgtggagg
     1741 ctggcgcggt ctcgcgctgt aattctgtgc ggtacacggc ttcggcgcgc gcgatgtagg
     1801 cctggtaacc tgcgggatcg atgaatggat ttgccgcggg gtgtttctcc atggcttcgc
     1861 gcttttgctt cagagagtag aaggagccgt gggctcccaa aaaaacgtca catggaagag
     1921 atctcaggac gcggaaagtt ttttcgtagt cggcttgaat gcccgagtaa gttgccgggg
     1981 tgttggggcg atcgatcaat ttgtaatttg ggaggacgct ggcgctgccg acgaagacta
     2041 cgttgtacga tttgccgtta tcgcgtgcga tcatcgacca agtggtgcag cctcgagtgt
     2101 ggccaggtgt gagatgcgcg gtcatcgtta cgccgcccag cgagactgta tcgccgtcgt
     2161 gaatcactcg gtcgggcgtc acggcaggaa acgatttgcc gctggtgaga gtgggtactt
     2221 cttcggccat ggccacgagt tgggcgccgg tggagcgctt gagttcggcg aaacccccgg
     2281 cgtgatcgag atgttcgtgg ctgcttatca gaaatttcac gtcagccaat ttgaagccga
     2341 gagttttgat gttggcctcg atttgcggag cggtttcaac gaaaccgcca tcgagcaaga
     2401 tatcgccagc ggacgtgacg atgagataag aagtgatatc cgatgcgccg acgtaataga
     2461 tgttgccgat gatgtggaac ggtggcacgg gctggttcat gttgcgagaa agatcgttgg
     2521 cctgtgctcg cacgaacgcg ggaaaggcca tcacaatggc gagagcgaga gcccatcgaa
     2581 gcgcgcacat cctttgtttg ctcccggcgg tcatcatggc gattatctgg cctccgcttg
     2641 cgttggagac gctagcaatt tctgataggt ggcttcgccg cggtcaacga aggctttgca
     2701 gccggtggga tcgatgaagg ggttgggggc gttgggatcg ctggtcagct cttggtgctt
     2761 tttttccatg tcatagaaag acgagttgga gccgaggaag acatcgcatt tcagcatggg
     2821 gagcacttgc atcgttttcg cccagtcggc tcccatggaa ggataggcgg ccgttccttt
     2881 cagattcgtg ttcggagaca gagtgacatt gccaaagaaa acgacgttgt aggatttgcc
     2941 gtctttgtct gtggtgacca tggtccaggt ggtgttgccc ggcgtgtgac ccggagtgcg
     3001 atgcgccgtg agggtgacgc caccgagctt cacctgatca ccatcgtgaa cgacgcgatc
     3061 gaccttcacg gcgggaaaaa ggtttctgtc gccgaacagc acatcattgt ggccgccgtt
     3121 ttcaaagaaa ggcgcgtcct cggccatcgc gacgagcttt ccgccggtgg ccgctttgat
     3181 cgcggccatg ccgccgccgt gatcaatgtg ggcgtgagta ttgatcagca gtttggtgtc
     3241 tttcaacttg aaaccgagct tttcgatatt gccttcgatc tgcggggcca tatcgggata
     3301 accggtatcg aggaggatgt tgcctcgcgg cgtgttgatc agatagcaag agagacccgc
     3361 ggagccgaca taatagatgt tgccaatcat gtgaaagggc gggaagggat ttttcatggc
     3421 agctgctgca gccgcggcac cgcctgcagc agtctgtcct tgcgggggcg atccggcggg
     3481 cgcctgggct atagccgcac cgcaaatcag agtggcggta gcgagcgcga gagatgaaag
     3541 accgatgcgc cgcgccgata atctattcga agtcattttc cccccttgcg ccgcaaagct
     3601 agcagaaccg gagttgcgtg gcaaatggtt gctgtgacga ctcagcggtt cgctgtgtta
     3661 ttgacaggcg cacgatgcga ccagtaatat tccggccacg ttgcggtcaa ccaagttcat
     3721 caggtagcgg gagaaacgac catgaagaat gcccttctag cggtaattgc ggctttgtgc
     3781 gtgtttcttt acacgggaac gggaacgaaa gctcaagatc aggctgcgcc gcctccgcct
     3841 ggggcgccga ctacggcggc agccgcgccc ggagcgactg cgccgcaggg gcgcaagaag
     3901 cgtatcgccg tattcgattt cgactacgcg acagtgcaaa cggcgtcgtc cgccgcgttt
     3961 ggcaccaacg tcgatgtggg taaagggatc acggatctgc tggtgaagta tcttgttcag
     4021 gatggaactt actcggtaat cgaacggcag gcgctggaca agatcatggc cgagcagaat
     4081 ttctccaata gcgaccgcgc caacccgacc tcggcagcga agctcggcaa gctgctgggc
     4141 gtggatgcga ttattgttgg cgctgtgacg cagtttggca acgacgctaa gaaccagaac
     4201 atcggcggcg gaggcggcaa ttggggcggc ttcggcgtcg gaggcttttc gcacaagaag
     4261 accaaggcga ttgtgaacat cgatgcgcgc atcgtggaca tcgacacagc agagattttg
     4321 ggcgtcgccg agggccatgg cgaatcgtcg cgctccagca cggggctatt gggaggcgga
     4381 ggaaactggc acggctgggg caatggagcc gtggatttcg gcagcagcga cttccagcag
     4441 accattctag gcgaggcggt caaggcggcg gttgagcaga tgagcaccgg cctagtggcc
     4501 gataacacca agctgcaaac gcgcacgatt gttgtttcgg gcctcgtggc gtccgtcgac
     4561 ggcggacaga ttgttttgaa tgtgggcggg aaggccggct tgaaggtggg cgatcagcta
     4621 tcggtcgagc gggtcacgaa ggagatcaaa gacccggcga ccggagcggt gatccgtcgg
     4681 ctggcttcgc ccgtgggcgt ggtccgtgtt acagatgtgg acgatatttc ggcggtggcc
     4741 gcgcctgtct ccggagcggg attcaaggtg ggcgatgcgg ttaaaaccgt gacccagtag
     4801 gaccgctcgg ctcgttaaaa ccgtgatctt tgttcgcgcg aggagcacgc ccgggtgagc
     4861 ccgggcggca aacagcggcg gcgagccgcc gcactccaca tgaatccgaa tgggcgcagt
     4921 acgaggatac gagcaatatt ttgccgcggg cacgtaagcg cgcgcaagta gttgttggtg
     4981 taaagaaaaa tcgtccacgg tgcgtgtggt gtttcg
//
LOCUS       DATNDT010000040         3911 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2226258, whole genome shotgun sequence.
ACCESSION   DATNDT010000040 DATNDT010000000
VERSION     DATNDT010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2226258"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2149
                     /locus_tag="VN788_01365"
     CDS             <1..2149
                     /locus_tag="VN788_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosidase"
                     /protein_id="HXU19210.1"
                     /translation="EYAKASAEDVLIRITAWNRGPEPARLYILPTLWFRNRWAWGENY
                     DPPKAYRIDAPVKTSLIEISEYHYGKRWLLLEGAPELLFTDNETNAERLFQARNRTPY
                     VKDAFHRYVINGEKSAVNPARKGTKAAALYAADIAPAKSWTICARLMDQTPPHNPADA
                     GQFFGTSFDKVFVQRVKEADEFYSQRAHVGMGDDARLVQRQAFAGLLWGKQSYHYDVR
                     RWLQGDPTQPTPPPERREGRNHQWVHLHNSDVISMPDKWEYPWYAAWDLGFHCVAMAV
                     IDPDFAKDQLILFLREWYMHPNGQLPAYEWNFSDVNPPVHAWAAWRVYKIAGRIQGKE
                     DRNFLERVFHKLLLNFTWWVNRKDPDGKNVFEGGFLGLDNIGVFDRSRPLAPGTTIEQ
                     SDGTSWMAMFCLNMLAMAMELARDDPAYEDVASKFFEHFVFISQAMNDMGGEGIGLWD
                     EKDGFYYDVLHRADDGHLQMKVRSMVGLIPLFAVETFDPEDLARLPAFTRRMQWFLNH
                     HAEVSEHVDMSQRTESGARLLLTIANRKKLERIYRYVFDEKEFLSPHGIRALSKFHRD
                     HPFILNVDGQPHSVDYEPGESTSDLFGGNSNWRGPVWFPMNFLLIESMQRVHFYYGDE
                     FQVDFPTGSGKKKTLWNVASELSRRLSHIFLRGNEGRPVYADTALFQQDPFWSDLILF
                     YEYFHGETGAGLGASHQSGWTALVAKLIEQSGA"
     gene            complement(2165..2782)
                     /locus_tag="VN788_01370"
     CDS             complement(2165..2782)
                     /locus_tag="VN788_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19211.1"
                     /translation="MAEDPDLAKWVSSLADSEPSKRQAAARQLHAAGTALCFSATREW
                     FKDAEFCELVRPFFADASGEPSRGNIPFSAGIAVQPVNFERIRAANGSPRLADVPPDQ
                     DAMEFEFRWGEHEEFDILTTRDPGGPGAIARYLEKFGEGIQQIEVNVTSVDRATEILR
                     TRFGLTPVYPRTRAGANGTHVNFFLAPAAEGRRVLIELVEAALKK"
     gene            complement(2790..3653)
                     /locus_tag="VN788_01375"
     CDS             complement(2790..3653)
                     /locus_tag="VN788_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19212.1"
                     /translation="MKSKISVVSILVAVMIAVVPRATAQDGSIEFVAHATPSGGLEEP
                     VRGFPFYLLSKSFEEIGKEADADDPKPDLNQFIDKLDSSSYSPQLKAWMKKNQFVTLS
                     GEDFIRKLHPGDVMDIPEFFSAYMNRNAGDQSADFPKSRVKASDKIKNPARYEKATAE
                     YMEMIRHYIEQHSQSVDGIDLNLSDIDPGPKWNALLGKRTPEIHRRALDLAQSKYLVA
                     RAESNLQGQGFLRDVPPGSYWISTLDVAADVGDARPRWDVPVTVQPGRTEYVSLSNVN
                     SVRSSPSHPNQ"
BASE COUNT          826 a         1094 c         1117 g          874 t
ORIGIN      
        1 ggaatatgcg aaagccagtg ccgaggatgt cttgatccgc ataacggctt ggaaccgcgg
       61 gcccgagccg gcacggctgt atattttgcc cacgctgtgg tttcggaatc gctgggcatg
      121 gggcgagaac tacgatccgc caaaggcgta tcgcatcgac gccccagtca aaacatcgct
      181 gatcgagatc tccgaatatc attacggcaa gcgctggctg cttctagaag gcgcgcccga
      241 acttctcttt accgacaatg aaacgaatgc ggagcgtctt ttccaggctc gaaaccgcac
      301 tccgtacgtg aaggatgcgt tccatcgata cgtgatcaac ggcgagaagt ctgcagtgaa
      361 tcccgcgcgg aaaggcacta aggccgccgc cctatatgcc gccgacatcg cacccgcaaa
      421 aagctggacc atttgcgctc gtctgatgga tcagaccccg ccacacaatc cggcggacgc
      481 ggggcaattc ttcggaacaa gcttcgataa agtgttcgtg caacgagtta aggaagctga
      541 tgagttctac tcccagcgcg cccacgttgg aatgggcgac gatgctcggt tggtgcagcg
      601 ccaggcgttc gctgggctgc tatggggcaa gcaaagctat cactacgatg tgcgtcgctg
      661 gctccagggt gaccccactc agccgacgcc tccgcctgag cgacgcgaag gtcgaaatca
      721 tcagtgggtc catctccata attccgacgt gatttcgatg ccggataaat gggagtaccc
      781 ctggtacgcc gcttgggatt tggggtttca ttgcgtggcg atggctgtga tcgacccgga
      841 ttttgcaaag gaccaactta tcctgttcct gcgcgaatgg tacatgcacc cgaacggtca
      901 attgccggcg tacgaatgga atttttcgga tgtaaatccg ccggtgcacg cgtgggcggc
      961 ttggcgcgtc tacaaaatcg ctgggcggat tcaaggcaag gaagaccgca acttcctgga
     1021 gcgggttttt cacaaactgc tgttgaattt cacgtggtgg gtaaacagaa aagacccgga
     1081 cggcaagaat gtgttcgagg gcggctttct cggattggac aatatcggag ttttcgaccg
     1141 ctcgcgccca ctcgctcctg gcaccacgat tgagcaatcg gacgggacca gttggatggc
     1201 catgttctgc ctgaatatgc ttgccatggc gatggagttg gcacgcgacg atccggctta
     1261 cgaggatgtt gccagcaagt tcttcgagca cttcgtcttc atctcgcagg ccatgaacga
     1321 tatgggcggc gagggcatcg ggctgtggga tgagaaagac ggcttctact acgacgtgct
     1381 gcatcgggcg gacgatgggc accttcagat gaaggtgcga tcgatggtgg gccttattcc
     1441 tctcttcgcc gttgaaactt ttgatccgga ggacctggcg cgcttgcccg cattcacgcg
     1501 ccgcatgcag tggtttctga atcaccacgc tgaagtatcg gagcacgttg acatgagcca
     1561 gcgcacagag agcggcgcgc ggcttctgct caccattgcc aatcggaaga aactcgagcg
     1621 aatttatcgc tatgtcttcg acgaaaagga attcctctct ccgcatggga ttcgggcgct
     1681 gtcaaaattc catcgagatc atcctttcat tctcaacgtg gacggacagc ctcattcagt
     1741 ggattacgag cccggcgaat ccacgagcga tctcttcggt gggaattcca attggcgcgg
     1801 gcctgtttgg tttccgatga acttcctcct gatcgaatcg atgcagcgcg ttcacttcta
     1861 ttacggcgac gaatttcaag tggacttccc caccggctcg gggaagaaga agacgctttg
     1921 gaatgtggca agcgagctct cacgccggct ttcacacatt tttctccgcg gaaatgaagg
     1981 tcggcctgta tatgccgata cagcactgtt tcagcaggat cctttttgga gcgatctaat
     2041 cttattctat gaatattttc acggcgaaac gggcgcgggg ctgggcgcaa gccatcaatc
     2101 cgggtggacg gctttagttg cgaagcttat cgagcagagc ggcgcataac ccagcggccc
     2161 tagcttactt tttcaaggcg gcttccacca actcgatcag cacacgcctg ccttctgcgg
     2221 ccggcgcgag aaaaaaatta acgtgtgtgc cgttcgcacc cgcacgcgtc cgcggataaa
     2281 ccggtgtaag gccgaaccgc gtccgcaaga tttccgttgc tcggtccacg ctggtcacgt
     2341 tcacttcaat ttgctgaatt ccctcgccga atttctcgag gtaacgcgca atggcacccg
     2401 ggccgcccgg atcgcgcgtg gtcagaatgt cgaattcctc gtgctcgccc cagcggaact
     2461 cgaattccat cgcgtcctga tccggcggaa cgtcggcgag ccggggcgag ccgttagcgg
     2521 cacggattct ctcgaaattt accggctgca ccgcaatgcc tgcgctgaaa gggatgtttc
     2581 cccggctggg ctcgccgctt gcgtcggcga aaaacgggcg cacgagttca caaaattcag
     2641 cgtccttgaa ccactcccgt gttgcggaga aacacagcgc ggttcccgcc gcgtgcagtt
     2701 gccttgcggc cgcctgcctt ttcgacggct cggaatcggc caggctgctt acccacttcg
     2761 caagatcggg gtcttcagcc atgtttgtgt cactggttgg gatgactggg cgatgatcgc
     2821 acggaattaa cgttcgaaag cgagacgtat tcggttcttc ctggctgcac ggttacaggc
     2881 acgtcccatc gcggccgagc atcgccgacg tccgcggcga catcgagtgt actgatccag
     2941 tagcttcctg gcgggacgtc gcgcaaaaat ccttgccctt gaaggttcga ttccgcgcgc
     3001 gcgaccaaat atttagactg agccagatca agagctcggc gatgaatttc gggagtccgt
     3061 tttccgagca aagcgttcca tttgggcccg gggtctatat cagaaagatt gagatcgatg
     3121 ccatcgacgc tttgcgaatg ctgctcgatg tagtggcgga tcatttccat gtactcggcg
     3181 gtggcttttt catatctcgc cggattcttt attttatctg aggctttcac cctggatttg
     3241 ggaaaatccg cggattgatc gcctgcgttt cgattcatat aggccgagaa aaattcggga
     3301 atatccatga cgtctcccgg atgcagcttg cgaatgaagt cttccccact cagcgtcacg
     3361 aattggttct tcttcatcca ggctttcaat tggggcgaat aggaagagga gtcgagtttg
     3421 tcgatgaatt ggttcagatc cggtttcgga tcatccgcat ccgcctcctt cccaatttcc
     3481 tcgaagcttt tgctcaagag ataaaaaggg aagccgcgta ctggctcttc caggcccccc
     3541 gagggagtgg catgcgccac gaactcgatc gagccgtctt gcgcagtggc tcgcggcacg
     3601 accgcgatca tcaccgctac caggatcgag acaacgctga ttttgctttt catggcggtc
     3661 atcgtaccag agcggtacga cggggggctg caccgcgcgg aacgatgggt acagttggtt
     3721 aacgtccggc ttggctgatg ctcatggaaa acaaaaatcg ctgaaggcga aaatacactc
     3781 gcgccttcag aacaattgaa acgcaacttc gataatctgc cgcaccgcag cgggcctccc
     3841 atcgggcccg cgcgcaggcg tgagccgcca ggtccgcacc gcctggatag ccttttcatc
     3901 gaggccaaga c
//
LOCUS       DATNDT010000041         2001 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3087989, whole genome shotgun sequence.
ACCESSION   DATNDT010000041 DATNDT010000000
VERSION     DATNDT010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3087989"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            230..>2001
                     /locus_tag="VN788_01380"
     CDS             230..>2001
                     /locus_tag="VN788_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU19213.1"
                     /translation="MKLTKGFVRALIFAIVAVGIAFLMDAVMARAQAGAKQGASATAT
                     AHTQWSDFGGGPDSAQYSSLTQINPSNVSKLQVAWTSPTGDEARYFFEPVQAHGVVYV
                     MSAGHSISAIDAETGEQLWQWKPEDKTTAMTDRGINYWESKDGKDRRLIFARNQLLQE
                     LDAQTGKPITAFGTNGTIDLREGLGRDTSKLTVVQSMTPGRVFENLLIEGSATNQGWG
                     SAPGDIRAFDIPTGKLVWTFHTVPHPGEPGYDTWPKDAWKTVGGANSWGEISLDAKRG
                     IVFIPTAAPKYNFYGGDRHGADLYGDCLLALDARTGKLLWYFQMVHHDIWDYDNTSAP
                     KLLTVIHNGKPEDVVAQAGKTGWVYVFDRVTGKSLWPIVEKPEPKSDMPGEETYPTQP
                     IPTAPPPFARQSFTSKELNPYLPPEEAAKWKQQIDASRNDGMFTPPDMNDTMEMPGNA
                     GGANWGNTAADAKNGIFIVASMDFPTILHLQVPDAPAAGSAAQDPPSNGITQYYMRGF
                     GYMNTSLGVPPITPPWSTLTAYDLNKGTIKWQIPLGEVPSLAAKGIHNTGAGQMKSGP
                     VITASGLVFTATRDHFIRAYDEQTG"
BASE COUNT          468 a          581 c          575 g          377 t
ORIGIN      
        1 agaaatcaaa tctcaaattg ctgatccagg aaagcgtatc tacctatccc aatttccagg
       61 cgaaaccgca ttttccgcct cagaatggcg cgggcgtgcc ggcgagtgtg tggtgctcct
      121 cgaaaagcac cactagtctt aaatcaactc ccaatgcgaa ttcgcgtagg gagccttagg
      181 ccaacgtttg gtgtaggcta caggggctca gttctgtggg gaggattgga tgaagttgac
      241 caaagggttc gtgcgggcgt tgattttcgc gattgtggca gtcggtatag cctttctgat
      301 ggacgccgtg atggcaagag cgcaagcagg agcgaagcaa ggcgcatcgg caacggcaac
      361 agctcatacg caatggagcg acttcggcgg cggcccggat tctgcgcaat attcctcgct
      421 cacgcaaatc aatccatcga acgtaagcaa gctgcaagtc gcatggactt ccccaacagg
      481 tgacgaagcg cgttactttt ttgagccggt gcaagcgcat ggcgtcgttt acgtgatgag
      541 cgcgggccat tcgatcagcg ccatcgacgc ggagacgggc gagcaactgt ggcaatggaa
      601 accagaagac aaaacgaccg cgatgaccga ccgcggcatc aactactggg aaagcaaaga
      661 tggcaaggac cgtcgcctga tcttcgcgcg caaccagctc ttgcaggaac tcgacgcgca
      721 aacaggcaag ccgatcaccg cgtttggcac gaacggcaca atcgatctcc gcgaagggct
      781 cggccgcgac acgtcaaaac tcaccgtcgt gcagtccatg acgcccggcc gcgttttcga
      841 aaatctcctc atcgaaggat cggccacgaa tcagggctgg ggctcggcgc ccggcgatat
      901 tcgtgcgttt gatatcccga ccggcaaact tgtttggacc tttcacacgg tcccgcatcc
      961 gggcgaaccc ggttatgaca catggcctaa agatgcatgg aaaaccgttg gcggtgcgaa
     1021 tagctgggga gaaatctccc tcgatgccaa gcggggaatt gtcttcatcc cgacggcagc
     1081 cccgaaatac aatttttacg gcggagaccg ccacggcgcg gatctctacg gcgattgcct
     1141 gctggcgctc gatgcgcgca cgggcaaact gctctggtat ttccagatgg tccatcacga
     1201 tatttgggat tacgacaata cgtcggcgcc gaagctcctt acggtaatcc acaacggcaa
     1261 gccggaagac gtcgtcgcgc aggccggcaa aacgggttgg gtttacgtct ttgatcgcgt
     1321 cacgggcaaa tcgctctggc ctatcgtgga aaagcccgag ccgaaatcgg acatgccagg
     1381 agaagagacg tatcccacgc agccgattcc gaccgcgccg ccgccctttg cccgccaatc
     1441 gttcacgtcc aaggaattga atccctatct tccgcctgag gaagcggcca agtggaagca
     1501 gcaaatcgac gcctcgcgca acgatggaat gttcacgccg cccgatatga atgacacgat
     1561 ggagatgccc ggcaatgcag gcggtgccaa ctggggcaac acggcagccg acgcgaagaa
     1621 cggtattttt atcgtggctt cgatggattt tcccacgatc cttcacttgc aagttccgga
     1681 cgcgcccgcc gcaggttcag cggcgcagga tccgcccagc aacgggatca cgcagtatta
     1741 catgcgcggc ttcggctaca tgaacacgag cctcggcgtg cctccgatca cgccgccttg
     1801 gtcgacgctg acggcctatg atttgaacaa aggcacaatc aaatggcaga taccgctcgg
     1861 cgaggtgcct tctctggctg cgaaaggcat tcacaatacc ggcgcaggac aaatgaaatc
     1921 cggcccggtc atcaccgcaa gcggcttggt tttcacggcg acgcgcgatc attttatccg
     1981 cgcctacgat gagcaaaccg g
//
LOCUS       DATNDT010000042         2599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3398357, whole genome shotgun sequence.
ACCESSION   DATNDT010000042 DATNDT010000000
VERSION     DATNDT010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3398357"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..2047
                     /locus_tag="VN788_01385"
     CDS             329..2047
                     /locus_tag="VN788_01385"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU19214.1"
                     /translation="MRVICLARKVLILFLVSAGTSPQTLPQEVPRVGITTPSPHASAS
                     QERTRSSIEGLVVRLGTSEPIRKAHVDVAPVNGGGTYRRLTDASGRFVVDGIEPGRYK
                     IRIHKVGYVGYLGQDYGEDATAGSGESLTVHPGENISDLLFRMVPEGVISGRIADEDG
                     APVVDASVTALLMQVRRGQRKLFAIATVQTNDLGEYRLYDLRPGRYYVRAARQEQYVK
                     EKKQPKNNDAAAAQSAYTAVFYPGTSEAKDAIAITVPPGQEVPLGDMILVPTSAVRLR
                     GHVFNAISGKPLTGCCVFLELGGGVVLDDSGPGRLAGPDGAFEIDNVVAGSFVLRASA
                     LTDGKWHDARLPIEVGNVGLNDLNLTIFPEVTITGRVAIEGSESVRLSTLHVVTEELE
                     CGFCTNGRPADVKTDGTFQLIDLPPGKYQVRVQGGPPAAYLKSVHFQGESSPDNVLNV
                     HPGAKQGPLEILLNPAGCQVDGSVTDRDGLPLPHATVVLVPDKQRRQFYSLYKDAKTD
                     QAGDFILTGIAPGDYEVFAWNDIDNDEWEDPDFLGPLEPYATRVNDEDCERKSISLKV
                     IQWSER"
     gene            complement(2152..>2599)
                     /locus_tag="VN788_01390"
     CDS             complement(2152..>2599)
                     /locus_tag="VN788_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXU19215.1"
                     /translation="LRDENKPMPSLILIDGGIGQLHAAAQALEKLEIINQPMASIAKK
                     EEILYVLGREDEPCVLDHHSPVLHLIQQIRDESHRFAVTFHRQRRSSRRIRTALVEIP
                     GVGDRTAQKLLRRFGSVTRLRQLTLDELTAELPRAQAQRIFDALRQ"
BASE COUNT          616 a          670 c          744 g          569 t
ORIGIN      
        1 ggcgcaaaaa gaactccgat aagcgccccg attcccatgc ccaccaggaa aaaactgatc
       61 ttcgacccag cattgtccgc catgttgcac ctcctatttc gacagtagca tagccccgaa
      121 gtgacaggca aggatagtgc cgggtcaggc tgaattcgac aacgtgccat ttctcgcgga
      181 actaagactg gaggttggta tccgcctaag ccaattgcac cataatcctc gaagtggtaa
      241 taaagtcaga acagagaaga gttatgttgt ctcgctgcaa tcgaatcatt gttttgttta
      301 gaaagtattt gagtagttag agaaccccat gcgtgtcatc tgccttgctc gaaaggtgtt
      361 gattcttttt ctcgtatcgg ccggaacgtc cccgcaaact ctaccgcagg aagttccacg
      421 agtaggaatc accacgcctt ccccgcacgc gagcgcttcg caggaacgaa cgcgaagctc
      481 catagagggc ttggtcgttc gcttaggtac aagcgagccg attaggaaag ctcacgtcga
      541 cgttgcccca gtgaatggcg gtgggactta tcgccgactc acggatgctt ctggtcgatt
      601 tgttgtggac ggaatcgaac ccggtcgcta caagattcga atccataaag tcggttatgt
      661 cggctatttg ggccaagact acggcgaaga tgcaacggcc ggctccggag agtctctgac
      721 cgtacatccc ggcgaaaata tcagcgatct tttatttcgt atggtacctg aaggagtaat
      781 ttcaggccga atcgcagacg aagacggtgc gcccgttgtg gacgcctcgg tcaccgcact
      841 tctaatgcag gttcgccgcg ggcaacgcaa actctttgca attgcaaccg tgcaaacaaa
      901 tgatttgggc gagtatcgcc tctacgattt gcgcccgggc cgatactacg ttcgtgcagc
      961 acgccaagaa caatatgtca aagagaaaaa acagccgaaa aacaatgacg ccgcagccgc
     1021 gcagagcgca tacaccgctg tcttctaccc tggcacctcg gaggcgaaag atgccattgc
     1081 aattaccgtt ccaccaggac aggaagttcc gcttggtgac atgattttgg ttccaacctc
     1141 agcggtaagg ctacgcggtc acgtattcaa tgcgattagc gggaagccgc tgaccggctg
     1201 ttgcgtgttc ctagagcttg gcggaggagt ggtcttggat gatagtggac cgggcagact
     1261 tgcaggcccg gacggggcgt tcgagatcga taacgtggtt gctggatcgt ttgtattgcg
     1321 tgccagcgcg ttaaccgatg gcaagtggca tgacgcccgc ttgcccatcg aagtagggaa
     1381 cgtaggtctg aatgatctca atctcacgat cttcccggaa gtgacgatca caggtcgagt
     1441 cgccatcgaa ggaagcgagt ccgtgcgctt gtcgacgctg cacgtcgtca cggaagaatt
     1501 ggaatgtggg ttttgtacta acggcagacc cgccgacgta aagactgacg gaacgtttca
     1561 actcattgat ctgccgccgg gaaaatatca ggttagggtt caaggaggcc cacctgcggc
     1621 atacttgaag tctgttcatt tccaaggaga gagcagtccg gataacgtgc taaatgtcca
     1681 tcctggggcc aagcaaggtc ctctggagat cctgttgaat ccggctggct gccaggtgga
     1741 tggaagtgtc actgatcgtg acggcctgcc ccttccgcat gcaactgttg tgcttgtccc
     1801 cgacaagcag cgtcgacagt tttatagtct ttacaaggac gcaaagactg atcaagccgg
     1861 agattttatc ttgactggaa tcgcaccggg agactacgaa gtttttgcct ggaatgacat
     1921 cgataacgac gagtgggaag atcctgattt tctagggccg ctcgaaccat atgccacgag
     1981 agtgaacgac gaagactgcg agcgcaaatc catctcgttg aaagtgattc agtggtctga
     2041 gcgctgagcc caatcctcca caaggaaaag ctgtgcctag ccacgaatcc gcgtaacttc
     2101 cagcgaatgc caccgaaaat tgactcacca ccgcgcgcgc gacgaggttg gctattgacg
     2161 aagggcgtcg aaaatgcgtt gggcctgggc acgagggagt tcggcagtca gctcgtcgag
     2221 ggtgagttgg cgtagacggg tgacgctgcc gaagcgcctc agcagttttt gcgcggttcg
     2281 atcgcccacc ccgggaattt ctacgagggc agttcgaatg cggcggctgg agcggcgctg
     2341 gcgatgaaat gtgaccgcga aacgatgcga ctcgtcgcga atctgctgga tcaggtgcag
     2401 aacgggagaa tgatggtcca gaacgcaagg ctcatcttcg cgaccgagaa cgtacagaat
     2461 ctcctctttc ttggcgatgc tggccatggg ctggttgatg atttcgagtt tctccaaagc
     2521 ctgcgcggca gcgtgcaatt ggccgattcc gccgtcgatc agaatcaagc ttggcatggg
     2581 cttgttctca tcgcgaagg
//
LOCUS       DATNDT010000043         5319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3397878, whole genome shotgun sequence.
ACCESSION   DATNDT010000043 DATNDT010000000
VERSION     DATNDT010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3397878"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..898
                     /gene="pxpB"
                     /locus_tag="VN788_01395"
     CDS             212..898
                     /gene="pxpB"
                     /locus_tag="VN788_01395"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HXU19216.1"
                     /translation="MTEGVQFQPASDQSLLLYFGQHVTLEAHQRIRKLLRLLETEPVS
                     GIRNLHPAYCSLLVDFDALKLGHAELEAILQSYIERLDTLTLPNPREREIPTCYGGEH
                     GPDLNDVATLCGMTPTEAVELHASITYVVYFLGFVPGFAYLGELPEALATPRLVNPRR
                     STPPGSVGIAGNQTGVYPFATPGGWRLIGRTPLAMFRPDRKNMSFLSIGDRVRFTPIS
                     VARFAALENG"
     gene            895..1836
                     /locus_tag="VN788_01400"
     CDS             895..1836
                     /locus_tag="VN788_01400"
                     /inference="COORDINATES: protein motif:HMM:TIGR00724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HXU19217.1"
                     /translation="MSAIQVQAPGLLTSVQDLGRGGFGPIGVSPSGAADPISLRLGNR
                     LAGNVESAAALEMTLLGGTFLFPLGAILALAGSDFVATIDDSPIAIGTSIEVRRGQTL
                     RLGPTRSGARCYLCLQGGISVPAFLGSASTHLLSGLGGFNGRPLRAGDVLQVGAATKL
                     FRKRTVAPHITEHLSRRNLLRVTPGPQVDWFSESSLHSFYEGTYRVGEQSNRMGLRLE
                     GTPTAPRQAGEMITEGVSLGAVQVPAGGLPIILFVDQQTTGGYPKIANVISADLHRVG
                     QLRPRDEIRFEQVTFEAARSMLIEQERLLASRGAILE"
     gene            1833..2588
                     /locus_tag="VN788_01405"
     CDS             1833..2588
                     /locus_tag="VN788_01405"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HXU19218.1"
                     /translation="MKRIDLNCDMGEVPEAISDGTQESLMRYITSASVACGGHAGDAR
                     TMKATIEQALRWKLAVGAHPGYPDRANFGRVNLNVPRETISDSVFEQVRLLAEIAGGC
                     GANVTYVKPHGALYNQAATDRVLAQAIADGVGRWSRDVALVGLAGSPMLDVFQEAGFP
                     VAAEAFADRRYEADGSLRSRRFQDALIGDPAEAGQQALSIAERGVVVAVGGDEVIAKA
                     QTICIHGDTPGAARIAAEVAQTLRNARIELRPF"
     gene            2643..4871
                     /locus_tag="VN788_01410"
     CDS             2643..4871
                     /locus_tag="VN788_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXU19219.1"
                     /translation="MPELQSQVGKTFSHYRIVEKLGGGGMGVVYKAEDTRLRRTVALK
                     FLPEGSTDRGTLERFHREAQAAATLNHPNICTIHEIGEQQGQPFIVMELMEGDTLKHL
                     IEGKPLRMDRLLDLATQIAEALDAAHSKGITHRDIKPANIFVTKSGQVKILDFGLAKL
                     TTTRRMSAEAVGVSGQVTAVVEEHLTSPGTALGTIAYMSPEQARGEDLDARADLFSFG
                     VVLYEMATGKPAFEGHTSAVIFDGILHGTPKPPMRANPEVPAELDRIIGKALEKDRDM
                     RYQTAAEVRADLKRLKRDRDSAVRAEASRDTIAPTASSEKKSVAVLYFENLSSTKEDE
                     YFRDGMTEDIITELTSIKDLKLFPRPAMLAYRDKPVTAPQVGHELGAAYVLGGSVRRS
                     GTRLRITAQLVETNTGHTLWAQRFDRELADVLDVQDEIARSIAQAFRISLTPQEERTI
                     ARKPTGNSDAYDYFLRGRSYMRRQSLDFALQMFEQAIKLDPSFAAAHAGVAGVSGMFY
                     EYYGRDPKWIKRGEGAVERALEVDPQLAEGLAGRGRMYYAQEQYEKAIEFAKRAIARK
                     PDCEGAYDLLGRALFASGRFQEAADLIDKAVEANGDDYNVYIPYTNSLDRLGRSDVSR
                     RFREMEMLVLERQLEAVPEDVRARVLLAADYANIGRIEDATRHAEMAVALRPKDSGVL
                     YNVACTYGALGKKQEALAMLHRSKEAGYSNEDWIHQDPDLKCLHDDPEFIRLFPPRGT
                     KD"
     gene            4992..>5319
                     /locus_tag="VN788_01415"
     CDS             4992..>5319
                     /locus_tag="VN788_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXU19220.1"
                     /translation="MTKTTTHSTPKIAHLFHTRARYRVVGLCGLTVLATLASLGVCSQ
                     AQEQKFFEKGSAPSFTANQVDRGKAAYTNRCLSCHGENLNGGEFGPTLKGAAFKTHWS
                     SQSAGAL"
BASE COUNT         1110 a         1529 c         1571 g         1109 t
ORIGIN      
        1 cggtttggct gtcacactgg gacgccggct tccagccaag ctgctcggtg cgatttcgcg
       61 gcaaatcctg gcgaaccgct ggttctcgcg ccacgtcgtt ctagatgatt ggtttcttca
      121 tctcgacgaa cctgcactca agactttcat cccgctcgcc acacagccct gaaatttctt
      181 cgcgccgata tatactgtct gcaaagcatc gatgaccgag ggcgtgcaat tccaaccggc
      241 gagcgatcaa tctctcttac tttacttcgg acagcatgtc acactcgaag cgcaccaacg
      301 catcagaaaa ctgctgcgct tgctggagac cgaacccgtc agcggcattc gaaatcttca
      361 ccctgcgtat tgctcgcttc ttgttgattt cgatgcgctg aagttgggtc acgcagaatt
      421 ggaagcgatt ctgcagagtt acattgaacg gctcgatact ctaaccctcc caaatccccg
      481 agaacgcgaa attccgacct gttacggagg ggagcacggc ccggacctca acgacgtagc
      541 gaccttgtgc ggaatgactc cgactgaagc cgtcgaattg catgcctcga ttacttacgt
      601 cgtctacttc ctcggattcg ttcccggctt cgcgtatctc ggtgaattgc ccgaagcgct
      661 ggcgaccccg cgattggtga acccccggcg gagcacgccg cccggcagtg tgggtattgc
      721 aggtaaccag accggcgtat acccgtttgc gacgcccggt ggctggcgat tgatcggtcg
      781 cactccgctc gcgatgttcc gtccggaccg aaaaaacatg agttttctat caattggaga
      841 tcgcgtgcgc tttacaccga tatcggtcgc gcgttttgcc gcgctggaga acggatgagc
      901 gcgattcagg tccaagcgcc gggcctgctc acctcggtgc aggatctagg acgaggaggc
      961 ttcggtccca taggcgtttc gccatcgggt gccgcggatc cgatttcact ccgcctggga
     1021 aaccggctgg ccggaaatgt tgagagtgcg gctgcattgg agatgacgct tctcggcggg
     1081 acatttcttt ttccgctggg agcaatcctg gcgctcgcag gctctgattt cgtcgcgacc
     1141 atcgatgatt ccccgatcgc tatcggaaca tccatcgaag ttcggcgggg ccaaacgctt
     1201 cgcctgggcc caacgcgttc gggagcacgc tgttatttat gccttcaggg cggaatctcc
     1261 gtgccggctt ttctaggcag cgcgtccacg caccttttga gcggactcgg cggtttcaat
     1321 ggccggccgc tccgcgcagg agacgtattg caggtcgggg ccgcgacgaa acttttccgc
     1381 aagagaacgg tcgctcctca cataacggag catctgtcgc gtcgcaacct cttgcgcgtc
     1441 acgccgggcc cgcaggttga ctggttttcc gagtcgtcgc tgcattcgtt ttatgagggc
     1501 acgtatcgag ttggggagca atccaatcgc atgggccttc gcctggaagg aacgcctacc
     1561 gcaccgcgcc aggccggtga aatgattacg gaaggcgtct ccctcggcgc cgttcaggtg
     1621 ccggcgggag gcttacccat catcttgttt gtggatcagc aaaccaccgg cggctatccc
     1681 aagatagcga acgtgatttc tgcagacctc catcgcgtag ggcagcttcg ccctcgtgac
     1741 gagatccgat ttgagcaggt tacttttgaa gccgcgcgat cgatgctcat cgagcaggag
     1801 cgactattag cgtctcgggg ggcaattctg gaatgaagcg catcgacctg aattgcgata
     1861 tgggggaagt gccggaagca atttccgacg gcacacagga atccttgatg cgctacatca
     1921 catcggcgag cgtcgcctgc ggtggccatg ccggcgatgc gcgaaccatg aaagcgacca
     1981 ttgagcaggc gcttcgttgg aagctcgcag tcggcgcgca tcccggatat ccggatcgag
     2041 cgaattttgg ccgtgtgaac ttgaacgtgc ctcgggagac aatctctgat tccgtattcg
     2101 agcaagttcg actgctcgcc gaaattgccg gtggttgcgg tgccaatgtg acatacgtca
     2161 aaccgcacgg tgcactctac aatcaggccg caacggatcg cgtcctagcc caagcgattg
     2221 ccgatggggt cggccgctgg agtcgcgacg tcgcacttgt gggcttggct ggatcgccga
     2281 tgctggacgt ttttcaagaa gccggcttcc ctgttgccgc ggaggcgttt gcagaccggc
     2341 ggtacgaggc cgatggcagc ctgcgctcgc ggagatttca ggatgcgctg atcggcgatc
     2401 ctgcagaggc ggggcaacag gcattgagca tcgcagagcg aggagtggta gttgcagtcg
     2461 gcggcgacga agtcatcgcg aaggcgcaga ctatctgcat ccacggggat acgcccggcg
     2521 ccgcaagaat tgccgcggag gtcgcgcaaa cgctacgaaa cgcccgcatc gagctgaggc
     2581 cgttttaacg cttctgtacg agcgttgact tgttccgaag cgcaacctag aatgagcctc
     2641 ccgtgcccga actccaatcg caagtcggta agaccttttc ccattaccgc atcgtagaaa
     2701 agctaggcgg cggggggatg ggagtcgtct acaaggcgga agatacccgt cttcgccgca
     2761 cggtggccct gaagtttctc cctgagggat cgactgatcg agggactctc gagcgctttc
     2821 atcgtgaggc ccaggcggct gccactctga accatcccaa tatatgtacc attcacgaaa
     2881 tcggtgaaca gcagggccag cctttcatcg tcatggagtt gatggaaggc gataccctca
     2941 agcaccttat cgaaggcaag ccgctcagaa tggaccgtct tctcgatttg gccacgcaga
     3001 ttgcagaagc actggacgcc gcccactcaa agggaattac gcatcgggat attaaaccgg
     3061 ccaatatttt tgtcaccaag tccggtcagg tgaagatact cgacttcggt cttgccaaat
     3121 tgacaacgac acgccggatg agtgctgaag ccgtgggggt ttcaggccaa gtcacggctg
     3181 tcgtcgagga acatctgact agtccaggca cagcgctggg aaccattgcc tacatgtcgc
     3241 ctgaacaagc gagaggcgag gatctggacg cgcgtgcgga tcttttttca ttcggcgtcg
     3301 tgctctacga gatggctacg ggcaagcccg ctttcgaagg ccatacttca gctgtgattt
     3361 tcgacggcat cctgcacggt acgccgaagc cgcccatgcg cgccaatccc gaagtgccag
     3421 ccgagctcga ccgcatcatc ggcaaagcgt tggaaaaaga tcgggacatg cgctaccaga
     3481 ccgccgccga ggtgcgagcg gatctcaagc gcctgaaacg cgaccgggat tcggccgtcc
     3541 gcgcagaggc ttccagggac acgatcgcgc ctactgcatc gtctgaaaag aaatcggtcg
     3601 cggtcctata ttttgagaat ttgagcagca ccaaagaaga cgagtacttc cgcgatggga
     3661 tgaccgaaga catcatcact gagctcacca gcatcaagga cttgaagctg ttccctcggc
     3721 cggccatgct ggcctaccgc gacaagcccg tcaccgcgcc gcaggtgggc cacgaattgg
     3781 gggccgcgta cgtcctcggt ggaagtgtgc gccgatccgg gactcgcttg cgcatcacgg
     3841 cgcagttggt tgagaccaac accggccata cgctctgggc gcagcgcttc gaccgcgaac
     3901 tagcggatgt cctcgatgtc caagacgaga ttgctcgcag cattgcacag gcctttcgca
     3961 tcagcctgac accacaggaa gaacgcacca ttgcccggaa gccgacggga aattccgacg
     4021 catatgatta ttttcttcgc ggccgaagtt atatgcgccg ccagagtctg gacttcgctt
     4081 tgcagatgtt cgaacaggcc atcaagctgg acccttcatt cgccgccgct catgctggtg
     4141 tcgccggcgt gagcggaatg ttttacgaat attatgggcg cgatccgaag tggatcaagc
     4201 gaggcgaagg agccgtggaa agagccctgg aggtagatcc acaactggcg gaaggtttgg
     4261 ccggacgcgg acgcatgtac tacgcgcagg agcagtatga gaaggcgatt gagttcgcca
     4321 aacgggccat tgcgcgaaag ccggattgcg agggcgccta cgatcttctc ggtcgagccc
     4381 tctttgcctc gggccgtttc caggaagccg ccgacctcat cgataaggcc gtcgaagcca
     4441 acggcgatga ctataacgtt tacattccct ataccaattc actcgacagg ctgggcagga
     4501 gtgatgtgtc acgccgtttt cgcgagatgg agatgctggt gctcgagcgg caactcgaag
     4561 ccgtgccgga agatgtccgc gcgcgcgtcc tgctggccgc tgactatgcg aatatcggcc
     4621 ggatcgaaga tgctactcgc catgctgaaa tggctgtggc tcttcgccct aaggattccg
     4681 gcgttctgta caacgtggcg tgcacttacg gagctttggg caaaaagcaa gaagccttgg
     4741 cgatgttgca ccgttcaaaa gaggcgggct attcaaacga ggactggatt catcaggacc
     4801 cggacctgaa gtgcttgcac gatgatcccg aatttattcg cctctttcca cctcggggca
     4861 caaaggactg acgctgctct cgtttctcgg accgattttt tgtaacccga tagggttggt
     4921 tctcaccact gaccttgtgg tgcatttcag ttggcggtag taaagtaccg cgtgctgtcg
     4981 gcgagaacac gatgacaaaa actacgacgc actcgacacc aaaaatcgcg catttgtttc
     5041 acactcgggc acgctaccgc gtcgtcggtt tgtgcgggct gacggtgtta gcgacgctcg
     5101 ccagccttgg cgtatgttcg caagcgcaag agcagaaatt tttcgaaaag ggttccgctc
     5161 ccagctttac cgcgaaccag gtggaccgcg ggaaagcggc ctacaccaat cgctgtttga
     5221 gttgccacgg tgagaatctg aatggcggcg aatttggccc gacgctcaag ggagcagcgt
     5281 tcaagacgca ctggagtagc caatccgccg gagctctat
//
LOCUS       DATNDT010000044         9942 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3653276, whole genome shotgun sequence.
ACCESSION   DATNDT010000044 DATNDT010000000
VERSION     DATNDT010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9942
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3653276"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            830..2215
                     /locus_tag="VN788_01420"
     CDS             830..2215
                     /locus_tag="VN788_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HXU19221.1"
                     /translation="MRRTFRLVFAIPIALLIVLLLTSSGAAAQDLQSKLDQYMQASVK
                     VNHFMGSVLVAQHGKILFAKGYGMANIKDRIPNASDTEFRVGSVTKEFTATAILKLQS
                     EGKLNVQDPVCKYVPNCPVDWHPIKIFHLLTHTSGIPDFTNFPNYLKIDTKPITPAQL
                     LADFKDKPLDFKPGAKFRYSNSGYEVLGYIIERVSGETYQRFLQQNIFGPLEMKESGY
                     DSSHPTAKNHAIGYVYAQTSFKPADYVDMTVPYSAGALYSTVQDLYTWDRALQAGKLL
                     PKPLLDQMFAPQVSTGGTADSHYGFGWFISTAYGHKEYSHEGGIQGFTCLNSWFPEQD
                     AYVIVLDNMQSAAIGTIARQLAAILFGQKYELPKAREAITLPPQELQKFAGQYQINPH
                     LMLTITLADGQLNLQSSGRPSLPIFPESKTEFFLKVADAQISFVLNPQGKATGLILHQ
                     GGQDMLGKKVN"
     gene            complement(2247..2831)
                     /locus_tag="VN788_01425"
     CDS             complement(2247..2831)
                     /locus_tag="VN788_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXU19222.1"
                     /translation="MDITDAQFRAQSELESGETLAWSGTADPRRAAMSALPAALFGIP
                     FAGFALFWMSQAFRATNAMAGNSHNSFASGFKIFPLFGIPFLLIGLSVTLMPLWAFLT
                     GKKTVYAVTNKRVMVISGSKNRAVKSFTPADIASVEHRERPDGSGDIVLMTNTLQRTG
                     NNMVAPMKVALCGVPNVKQVAEQVMALHTQSTRA"
     gene            complement(2941..3504)
                     /locus_tag="VN788_01430"
     CDS             complement(2941..3504)
                     /locus_tag="VN788_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HXU19223.1"
                     /translation="MRSSPRKFSTEDGLYNAATRALMRRAHSVHEMKDYLKARAENPE
                     HGAAVLQKLKEHNYLDDARYARDFARVHAQSRRQGKFRIAQELRRRGVPDRHIEAALD
                     SVFAETDEHALVRARLERKLRHLRGRASQNSANAANAPLDQKARASLYRSLLRAGFSP
                     DAIRAELRSLLQSASEEFEPPSPMGEF"
     gene            3609..4025
                     /locus_tag="VN788_01435"
     CDS             3609..4025
                     /locus_tag="VN788_01435"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXU19224.1"
                     /translation="MKQTMEENPIVTKRKRSGVDKYIAKCPKEVKGNLAKMRAAIRSA
                     APGAEERTDYFQWPGYSYSDRGFDYDGMFAWFTFKKPYIRLHVRPLVIQEHGKELAGY
                     PTTKAIVSFEMGKSIPIALVKKLVKGSIKVMKDKAK"
     gene            complement(4080..4427)
                     /locus_tag="VN788_01440"
     CDS             complement(4080..4427)
                     /locus_tag="VN788_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19225.1"
                     /translation="MPYIEPKHPWYTFFFGVRGLLRLFAAILAFAYAIFICAKLGTDG
                     PLFARISDAAIIAFSHTATLALVTGLFSIALWFGLHSSLEPGRWGFYQAFSEECEDYD
                     WWGRRLPNSSRRE"
     gene            complement(4792..5091)
                     /locus_tag="VN788_01445"
     CDS             complement(4792..5091)
                     /locus_tag="VN788_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19226.1"
                     /translation="MSFLIGLISAFLLWMVLQNDYYDYLYFPKVKAVDAYIYPQLRYT
                     IFDYHLDRGLPAAWTGSSSLSLGFAAFSDPMAFLTRHSAYCLSIALCLLLYFFMA"
     gene            complement(5178..6326)
                     /locus_tag="VN788_01450"
     CDS             complement(5178..6326)
                     /locus_tag="VN788_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005034251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXU19227.1"
                     /translation="MAVAVDDLLRKAVENKASDLHLKVGNHPYLRVDGVLQPIADVPR
                     VTPEEMLSMAFSMMTNRQKQKFKETAELDMAYGVAGLGRFRVNVFQQRGNVGMVLRVI
                     PTKIRTVEELNLPQVIDKICEEQRGLVLVTGTTGSGKSTTLAAMIDRINSLRPEHIIT
                     VEDPIEYLHRDKKGFINQREVEVDTSSFSTALRAALRQDPDVILVGEMRDLETISTAL
                     LAAETGHLVFSTLHTLDATETIQRIIAVFPPPEQKQIRLQLASTLKAVVSQRLVRKSD
                     GIGRVPAVEVLISTGYIRDCIINPDKTRLIRDALAAGTSQYGMQTFDQSIFDLYSKNL
                     ITLDEAMLRASNPDEFKLRIQGVRSASDAAREEMERQMQEFERTGPRR"
     gene            complement(6466..7203)
                     /locus_tag="VN788_01455"
     CDS             complement(6466..7203)
                     /locus_tag="VN788_01455"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HXU19228.1"
                     /translation="MYRIWQKLCAEFIGTFFFVLLSAGAVCADAALRSASTSATAGSA
                     ALGPLGIALVRGLAFAIFVSALYPISGGHFNPAITLGAWVTRSIGSISAILYGIVQLI
                     AAILASWTLTALVPGQIWRSVSLGAPDLGANISRTQGIFLELLLTFILVFVFSATVPD
                     VRSDSAPSSRFSTSASPAGFAAGLAIFVGYFAGAPFTGASMNPALALGTAVAAHHFAN
                     HGVYWAGPMLGGVLGAWVYHALFPRRS"
     gene            7392..7967
                     /gene="dps"
                     /locus_tag="VN788_01460"
     CDS             7392..7967
                     /gene="dps"
                     /locus_tag="VN788_01460"
                     /EC_number="1.16.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011144276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA protection during starvation protein"
                     /protein_id="HXU19229.1"
                     /translation="MGKVAREVVAKAGVNVDELVKKLIRAAAAELTTFYYYTILRVNA
                     IGFDGEGLKEIIEDARIEDRNHFEVLTPRIYELGGALPRDIKDFAKDAGCPDAYLPKD
                     PRDMKAMLGVLVEAERCAIRVYTEICALTTGKDPRTYDVALAILHEEIEHEAWFSEYL
                     GEGPSGHFRRGFPGESPYCAKFMVVPGARQK"
     gene            complement(7980..8120)
                     /locus_tag="VN788_01465"
     CDS             complement(7980..8120)
                     /locus_tag="VN788_01465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19230.1"
                     /translation="MLNNRAGALFNTYSLSIASPLSARRWLAFHGTRATGHITLFLAT
                     RH"
     gene            8155..8526
                     /locus_tag="VN788_01470"
     CDS             8155..8526
                     /locus_tag="VN788_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXU19231.1"
                     /translation="MPGNSESAGEKWVEVARVEDVPEGAVRGARANGRMLALMNLGGK
                     IYAVDGVCPHMKGPLWQGDVWQEQLECPWHHYRYDPATGRNTYPANVYPEDVPELQED
                     IQPVKTYKVRVEDGRIFVCYS"
     gene            complement(8812..9336)
                     /locus_tag="VN788_01475"
     CDS             complement(8812..9336)
                     /locus_tag="VN788_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HXU19232.1"
                     /translation="MPIVTFLRGVNVGGHRTFRPSLLAGKLNHLDFVNIGAAGTFVIR
                     KRVSQTLLHSELRKHLPFETHIMMCASRELLAAVSQNPFDGTLIRANLVRFVAVLAKR
                     SRALPALPLQIPREGKWFVRILSCHGRFVFGCYRREMKAIGYLGSMDKILGAPATVRN
                     WNTITQILRLLEQT"
     gene            complement(9655..>9942)
                     /locus_tag="VN788_01480"
     CDS             complement(9655..>9942)
                     /locus_tag="VN788_01480"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HXU19233.1"
                     /translation="DANGAYTFVLGAKVTSAKTIPSGMIAVKIPAGKYFVLTTDRGPT
                     DKVVPEAWARIWSLSKSQLGGKRAYKIDFELYGPHAADPQNAQVQIYVGIK"
BASE COUNT         2338 a         2483 c         3145 g         1976 t
ORIGIN      
        1 gccgccgtca ttccggtctc cgctcaatgt ctgttagagc agttcaaccc gcataattta
       61 ccgttcgctg caaatcgctt gcgccccgag tgatctccgt gctaccactg gccgatgtca
      121 tacgaaccaa ctctgctaac cagtggcctc cgctgctggc ctttcccagc ctcgcaattc
      181 tcgaagaccc tgacgcagga agggccaaaa tgcaacggac gccgccaact ctctcggtcc
      241 cttacccact cgcttccctt gaggaatgga gggcctctat ccgccagcca ctgctttcct
      301 aatcgcaaca ccccaaaatt aaaattcgcc gtaaatcaca caaaacaaag cctcgcccaa
      361 tttctaatcg ccacatttcg agccctcgca gcgatgcctc gagaacactg gcccgcccgc
      421 aatggttttt ccggagtgcg aaaattagaa aatcgcttaa cttgcttccc ttcagccact
      481 tccaaatttc taatcgataa cttttggccc gttttgtgcg acgcacttgc tcgtcacttg
      541 tttaccctga gatacgaagg gcttaccctg agagaggaag ggtcactgct tttctaatcg
      601 acagtccgat gaacctatga aaaattagaa gttggcctaa ctcgtttgcc atcaacaaaa
      661 gttatgattc taatcgacag tcaaatcgac ttcatccaag gcgcgattgt cgcgtcccga
      721 gcgaagggag ggatctgctt tccccactgc atcacccgcc gtgctaggaa ttcaagtgtc
      781 accagaggcc tagttgaggc atattctttc ttgtagggtg caacgcacaa tgagacgaac
      841 gttccggctg gttttcgcaa ttcccatcgc gcttctgatc gtgctcctgc tgacgagcag
      901 cggtgccgca gcgcaggatt tgcagtcgaa actcgaccag tacatgcagg cgagcgtgaa
      961 ggtcaaccac ttcatgggct ccgtcctcgt ggcgcagcac ggcaaaattt tattcgccaa
     1021 aggctacggg atggcgaaca tcaaagatcg catcccgaac gcgtcagata ctgaatttcg
     1081 cgtcggctcg gtcaccaaag aattcactgc cacggccatc ctcaagctcc aatccgaggg
     1141 caagctgaac gtacaggacc ccgtctgcaa atacgtgccg aattgtcccg tcgactggca
     1201 tcccatcaaa attttccatc tcctgacgca cacttccggc attcccgatt tcaccaattt
     1261 tcccaactac ctaaagatcg atacgaaacc gatcactccc gcgcagttgc tggctgactt
     1321 caaggacaag ccgctggact tcaagcccgg ggcgaaattt cgctacagta attcgggcta
     1381 cgaagtgctt ggctacatca tcgagcgcgt ttccggcgaa acatatcagc gattcctcca
     1441 gcagaacatc ttcggcccgc tggaaatgaa ggagagcggc tacgatagca gccatcccac
     1501 agcaaaaaac cacgcgatag gctacgttta cgctcagacc tcctttaagc ccgccgatta
     1561 cgtcgacatg accgttccct atagcgccgg cgccctctat tccaccgttc aggacctcta
     1621 cacctgggat cgcgcgctcc aggccggcaa gctgcttccg aaaccgttgc tcgaccaaat
     1681 gttcgcgccc caggtctcca cgggaggcac cgccgactct cattacggct tcggctggtt
     1741 catttccaca gcctacgggc acaaggagta ttcccatgag ggtgggatcc agggttttac
     1801 gtgcctgaat agctggtttc ccgagcaaga cgcctacgtt atcgtgcttg ataacatgca
     1861 gtccgcagcc ataggcacca tcgcacgcca gctcgccgcc atcctttttg gccagaaata
     1921 cgaactcccg aaagctcgcg aagccatcac cttgcccccg caagaattgc agaagttcgc
     1981 cgggcaatat cagatcaacc cgcacctcat gctcacgatc actctggccg atggccaact
     2041 gaacctgcaa agctccggcc ggccgtcgct gccaattttc cccgagtcaa aaaccgagtt
     2101 tttcctcaag gtcgcggacg cgcaaatcag ctttgtgctg aatccgcaag gaaaagcgac
     2161 tggcctaatt ctccaccagg gcgggcagga tatgttgggc aagaaagtca actagccgca
     2221 gagtccaaat tacttataca aaaaattcat gctcgggtgg attgggtgtg caaggccata
     2281 acttgctcgg cgacttgctt cacgttggga acgccgcaga gagcgacttt catcggagcg
     2341 accatgttat tgccagtgcg ctggagagta ttggtcatga gcacgatgtc gccggagcca
     2401 tcggggcgct cgcgatgctc gacgctggca atatccgccg gcgtgaacga tttgacagct
     2461 cgatttttcg agccgctgat caccatgacg cgcttattag tcacggcgta aaccgttttc
     2521 ttgcccgtga ggaaggccca gagaggcatg agcgtgacgc tgagtccgat gagcagaaac
     2581 gggatgccga acagcgggaa tattttgaat ccgctcgcga acgagttgtg cgagttgccg
     2641 gccatagcgt tggtggcgcg gaaggcttgc gacatccaga aaagcgcgaa gcccgcgaat
     2701 gggattccga aaagcgcagc gggcagggcg gacatggccg cgcggcgcgg gtcagcggtg
     2761 ccggaccacg cgagcgtttc gccggattcg agttctgact gggcgcggaa ctgggcgtcg
     2821 gtgatgtcca tgtcgcctcc tatttgcgta gggtaagtga atatgggcgc gaatgcgaca
     2881 gaagtagtaa ggagggtgcc cgaaaaagca cgatgcttgt aggcggcagg gagcccggat
     2941 tcagaactct cccataggtg agggcggctc aaactcctcc gaggcgctct gtagaagaga
     3001 tcggagttcg gcgcgaatgg cgtcggggga gaagccggcg cggagtaggc tgcggtagag
     3061 cgacgcgcga gctttttggt cgagaggcgc gttcgcggca ttggctgaat tttgcgaagc
     3121 gcggccacgg aggtgacgga gtttgcgttc gagacgcgcg cgaacgagcg cgtgctcgtc
     3181 ggtttcggcg aagacggaat ccagcgcggc ttcgatgtgg cggtcaggga cgccgcggcg
     3241 tcgcaattcc tgcgcgatac ggaatttgcc ctggcggcgc gattgggcat ggacgcgggc
     3301 gaagtcgcgc gcgtagcggg cgtcgtcgag gtagttgtgc tcctttaatt tttgcagaac
     3361 cgccgcaccg tgctcgggat tttcagcgcg cgctttgagg tagtccttca tttcgtggac
     3421 ggaatgcgcg cggcgcatca gcgcgcgagt ggcggcgttg tagaggccgt cttcggtgga
     3481 gaatttgcga ggactcgatc gcatgcggag agcataggag taaagaagga cggaagcaag
     3541 aaggacggaa gcgaggaggc agaaagccaa ggaggcaagg cgagtagaag tggggtcccg
     3601 aaggggcgat gaaacagacg atggaggaaa atccaatcgt gacaaaacgc aaacggagcg
     3661 gagtcgacaa atacatagcg aaatgcccca aagaggtcaa aggcaatttg gcgaagatga
     3721 gggccgcgat tcgctctgcg gcgccgggcg ccgaggaacg cactgattat ttccaatggc
     3781 cgggatattc ctattccgac cggggatttg actacgacgg aatgtttgcg tggttcactt
     3841 tcaagaagcc atacatacgc ttgcatgtgc gccctctcgt aatacaggaa catgggaagg
     3901 aactcgccgg ttaccccaca acgaaagcga tcgttagctt cgagatgggc aagtcgatac
     3961 caatcgcact ggtgaagaaa ctagtgaagg gaagtatcaa ggtaatgaag gataaggcta
     4021 aatagatttc gttatccact tcgacgtgta ctcaacgcag ggggaatgac cttcaaagac
     4081 tattcgcggc ggctggaatt gggaagacgg cggccccacc aatcataatc ttcgcactct
     4141 tcggagaagg cctggtagaa gccccagcgg ccgggttcga ggctgctgtg gagcccgaac
     4201 cagagcgcga tggagaaaag tccggtgacg agagcgagcg ttgcagtgtg actgaaagcg
     4261 atgatggcgg cgtcgctgat gcgcgcgaag agagggccat ccgtgccgag cttggcgcaa
     4321 atgaaaatgg cgtaggcgaa cgcgaggatc gcggcgaaga ggcgtaggag tccgcgaacg
     4381 ccgaagaaaa acgtgtacca aggatgcttg ggctcaatgt agggcatgtc ggaaaagtga
     4441 atctatcgca caaaatttcg ttaggcaaat attttaggaa tgaatgtgcg attttgtgca
     4501 atccggaaat ggataagcgt tggtgggttt ttgcgcgagg aaaaattcaa atgcagattc
     4561 ctcgtggtcc cgacgaaacc ggtcgggacc ttcggaatga cagtatgcag cgggcgcgca
     4621 gaaaaatgaa tgggagcgag gcccagatga ggcaaaccgg aaagcgaaat ccttagctac
     4681 gctcaggatg acaccttgaa cggagattcg cgttaagaac ggtgttggac ggcctatccg
     4741 gggttggcat tggaatgatt caaatgccat gccttctcat ttcaagcatg actatgccat
     4801 aaagaaataa agcagcaagc agagcgcaat agacaaacaa tatgcggaat gtcgcgtgag
     4861 aaacgccatc gggtctgaga acgccgcgaa tcccagtgac aaagaagatg agcccgtcca
     4921 ggcagcaggc aatcccctat caagatgata atcgaagatg gtgtatcgca gttgcggata
     4981 aatgtaagcg tcaaccgctt tgaccttggg gaaatacaag taatcgtaat agtcgttttg
     5041 aagcaccatc caaagaagga aggccgagat caacccgatg aggaaggaca atgagaacag
     5101 tatccatcgg cgtcccacaa acgtgccgat tctttcgccg agactcagga agtgttctcg
     5161 tccgtcgctg agtagcttta gcggcggggg ccggtgcgct cgaattcttg catctggcgt
     5221 tccatttctt cgcgggcggc gtcggaggcg gaacgaacgc cctggatgcg cagcttgaat
     5281 tcgtcgggat tggaagcacg cagcatggct tcgtcaagcg taatgagatt cttcgaataa
     5341 agatcgaaga tggattgatc gaaggtttgc atgccgtact ggctggtgcc ggcggcgagc
     5401 gcgtcgcgaa tgagccgcgt cttatcggga ttgatgatgc aatcgcgaat gtagccggtg
     5461 gagatcagca cttcgacggc gggcacgcgg ccgatgccgt cgctcttgcg cacgaggcgc
     5521 tggctgacga cggctttgag cgtggaagcg agctgcaagc gaatctgctt ttgctccggt
     5581 ggcggaaaga cagcgatgat gcgctggatt gtttcggtgg cgtcaagagt gtgcaaagtc
     5641 gagaaaacga ggtggccggt ttcggcggca aggagcgcgg tggaaatggt ttcgaggtcg
     5701 cgcatctcgc cgacgaggat gacgtcagga tcttggcgga gcgcggcgcg aagggccgtg
     5761 gagaatgagg acgtgtcgac ttcgacttcg cgctggttga taaatccttt tttgtcgcga
     5821 tgcagatact caatcgggtc ttcgacggtg atgatgtgct cgggccgcag cgaattgatg
     5881 cggtcaatca tagccgcgag cgtcgtggac ttaccggaac cagtcgttcc ggtgaccagc
     5941 acgaggccgc gctgctcttc gcaaattttg tcgatgactt gaggaagatt gagttcctcg
     6001 acggtgcgaa ttttcgtcgg aatgacgcgg aggaccatgc cgacgttgcc gcgctgctgg
     6061 aaaacgttga cgcggaagcg gccaaggcca gcgacgccgt aagccatgtc gagctcggcg
     6121 gtctctttga atttttgctt ctggcggttg gtcatcatgc tgaacgccat agacagcatt
     6181 tcctcgggtg tgacgcgggg cacgtcagcg ataggctgga gcacgccgtc aacacggaga
     6241 tacggatggt tgccgacttt caggtgcaag tcggaggctt tgttttcgac ggctttgcgc
     6301 agcagatcat cgacagcgac ggccataacg gttcaccttt tcgaggagca gatgcctcac
     6361 cgtagcatgg ggtacaggca gggacaatca ctgggccagt agcgtttgtc ggaaagggta
     6421 ctgtcctaaa ggctagataa gacgataagg agtaggcgtc gcgcgctatg agcggcgagg
     6481 gaagagggca tgataaaccc aagcgccgag gactccgccg agcatcgggc cggcccagta
     6541 gacgccgtgg ttggcgaagt ggtgtgcggc gacggcggta ccgagggcga gggcgggatt
     6601 catggaagcg ccggtgaacg gggcgccggc gaagtagcca acgaaaatgg cgaggccggc
     6661 ggcgaatcct gctggagagg cggaggtgct aaagcgcgag gaaggcgcgg aatcggagcg
     6721 cacgtcagga acagtggcgg aaaagacgaa gaccagaatg aaggtaagga gaagttcgag
     6781 aaaaattccc tgcgtgcgcg agatgttcgc gcccagatcg ggagcgccga gcgaaaccga
     6841 gcgccagatt tgtcccggga cgagtgcagt gagagtccag gaggcgagga tggcggcgat
     6901 gagttgtacg attccgtaga gaatggcgga gatggagccg atgctgcgcg tgacccaggc
     6961 gccaagagtg atggcgggat tgaaatgtcc gccggagatg ggatagagag cggaaacgaa
     7021 gatagcgaag gcaagcccgc gaacgagagc gatgccgaga gggccgaggg cggccgagcc
     7081 tgctgtggcg gatgtgctgg cagagcgaag ggctgcgtcc gcgcaaacgg cgccggcgga
     7141 aagcaggacg aagaagaaag tgccgatgaa ttcggcgcag agcttctgcc agatgcggta
     7201 cacgagggca cagcctcccg gaatcggggc cgattgtaca aggaagcaga agagcaagga
     7261 agcagagagg caaagaaatg ccgagcacag gtgtatgctt gcgtagtgga aactgatgcg
     7321 ggcaggggga agcattttca aggtgacgcc gaaattttca ggaaatctaa acgcacgagg
     7381 aggaaataaa aatgggtaaa gtagcacggg aagttgtggc caaggccgga gtgaatgtgg
     7441 atgagctggt gaaaaagctg attcgcgccg ctgcggcgga actgacgacg ttttattact
     7501 acacgatttt gcgcgtcaat gcgattgggt tcgacggcga agggctcaaa gaaatcatcg
     7561 aagatgcgcg catcgaagac cgcaatcatt ttgaagtact gacgccgcgc atttatgagc
     7621 tgggcggcgc actgccgcgg gacattaaag attttgcgaa agacgcggga tgtccggatg
     7681 cctatttgcc aaaggatccg cgagacatga aggcgatgct gggagttttg gtcgaagcgg
     7741 aacggtgcgc gattcgcgtg tacacggaaa tttgcgcgct gacgacgggc aaagatccgc
     7801 gcacgtatga tgtggcgctg gcgattttgc acgaagaaat cgagcatgag gcgtggttct
     7861 cggaatattt gggcgaagga ccatcgggac atttccgccg cgggttcccg ggcgaatcgc
     7921 cgtactgcgc gaagttcatg gtggtgccgg gcgcgcggca gaagtgacgt aagcagtggc
     7981 tagtggcgag tggctagaaa aagcgtgatg tggccggtgg cccgtgttcc gtgaaatgcg
     8041 agccatcggc gcgctgacag aggcgaagca atcgaaagcg aataggtgtt gaatagagcg
     8101 ccggcgcggt tgttcagcat acaagctccg gtggcgcagt ccttcatctg acgaatgccg
     8161 ggaaattctg aatccgcagg agaaaaatgg gtagaagtag cgcgtgtgga agacgtgccg
     8221 gaaggcgcag tgcgcggggc acgcgcgaat gggcgcatgc tggcgttgat gaatctaggc
     8281 gggaagattt atgccgtgga tggtgtttgt ccgcacatga aagggccgct gtggcagggc
     8341 gatgtttggc aggagcagct tgaatgtccg tggcatcact accgatacga tccggcgacg
     8401 ggacggaata cgtatccggc gaatgtgtat cccgaggatg tgccggaatt gcaggaggac
     8461 attcagcccg tgaagacgta caaggtgcgg gtggaagacg gaaggatttt tgtctgctac
     8521 tcgtgagaga gatgcaggcc cggcggacca cgcctggccc tacaaagcgt aacttgccgc
     8581 tcgtgcctcg atatcgttgg ggccgattac gccgatgtca cgtggtgcga tggaagtgaa
     8641 atgcctcagg tcagtcccgc aaaaatcgcg ggacgggacc tgagctacag ggagagtcgc
     8701 ggtcggcaga cctttggaat taccggcatt ggctctcgca ggccccacta aaaaaacgcc
     8761 tgcctgaagg ctgccgctac gatgtaagca gcgcaatcgg agagcagcgc gtcatgtttg
     8821 ttccaatagc ctgagaattt gcgtgatggt gttccagttg cggacggtgg caggcgctcc
     8881 aagaattttg tccatcgagc cgagatagcc tatagctttc atttcgcggc ggtaacaacc
     8941 aaaaacaaat ctgccgtggc aggagagaat gcggacgaac cattttccct cgcggggaat
     9001 ttgcaaggga agcgcgggca aggcgcggga ccgttttgcc agtacggcga caaaacgaac
     9061 gagattggcg cgaatgagcg tgccgtcgaa ggggttttgc gaaacagcgg cgagaagttc
     9121 gcgggaagcg cacatcataa tgtgcgtttc gaagggcaga tgcttgcgga gttcggagtg
     9181 gagcagcgtt tggctgacgc gtttgcggat gacgaaagta cctgccgcgc cgatattgac
     9241 gaagtcgaga tggttcaatt tgccggcgag gaggctgggg cgaaacgtcc tgtggccgcc
     9301 gacgttgacg ccgcggagga aagtgacgat tggcatggcg gcattatagg gaatttgatt
     9361 cgattggcaa cgtcttcgag tcgccgcagg aggacgtgtt tcttttgcaa atctgttgaa
     9421 catgagaatt cgtggggctc gacggatttg ctacaacgaa acagcgacgt acctcagagg
     9481 ctgaagccgc ttggtttgcg cgcgttcggc acagctaaag ccgggggcga aagctcactg
     9541 tcggctcggc gtccgttcct agctttatga gtgctctccc tgattggcgg catcttgtca
     9601 tacgtgacaa gaatgagtag attcgcggcc gaaatgaacg cgtggcgtac ggggctattt
     9661 tattccgacg tagatttgga cctgggcgtt ttgggggtcg gcggcgtgcg gcccgtatag
     9721 ctcgaaatcg attttgtagg cgcgcttgcc gccgagctgc gattttgaga gcgaccagat
     9781 gcgcgcccag gcttcgggaa cgactttgtc ggttgggccg cgatctgttg tgagcacaaa
     9841 gtattttcct gccgggattt tcacagcgat cattccagat ggaattgttt tggccgacgt
     9901 gacttttgcg ccgagcacga aggtgtaggc gccattggcg tc
//
LOCUS       DATNDT010000045         4901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1659574, whole genome shotgun sequence.
ACCESSION   DATNDT010000045 DATNDT010000000
VERSION     DATNDT010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1659574"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="VN788_01485"
     CDS             <1..807
                     /locus_tag="VN788_01485"
                     /inference="COORDINATES: protein motif:HMM:TIGR04305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CADD family putative folate metabolism protein"
                     /protein_id="HXU19234.1"
                     /translation="LAEMVLFYAMPRWTLCCPSGNIAPFYIPSLLKKGENSKIAMPKN
                     RAKTATMLESLDALIEQYHLLKHPFYQAWTKGTLSRESLQLYAEQYYQHVRAFPENLK
                     QFADRAEEPLAGLVEDNLAEELDPVSPHPALWRQFAESLGVSEASLEEAHPLPGIAAL
                     LDSYDEVVSQGTMAQAVAAFYVYEAQVPEIATQKISGLKRFYDISEPRALAYFGVHEE
                     ADVRHRAAWRQWLASQNEDDSLGVLCVAERSLKALWGALDAVYPEVCAKN"
     gene            complement(897..1607)
                     /gene="rpiA"
                     /locus_tag="VN788_01490"
     CDS             complement(897..1607)
                     /gene="rpiA"
                     /locus_tag="VN788_01490"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HXU19235.1"
                     /translation="MPIDASMQNEWKKVAAESAARLVEDGMAVGLGTGSTAEFFLTAL
                     AQRLSSERLRISGIPTSARTAALARELKIPLINFAERTRIDLTIDGADEVELSTLSLI
                     KGHGGALLREKIVAAASDRMIVVADETKVSGKLGTLASVPVEIVQFGWQVTLKKLGEL
                     AGTSELRLGGDGKPLITDGGNYIADCAFGPMDNPKETAHHLDHVIGAVEHGLFLGFAR
                     EVIVGGSDGAKIVKRSDK"
     gene            complement(1610..2728)
                     /locus_tag="VN788_01495"
     CDS             complement(1610..2728)
                     /locus_tag="VN788_01495"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HXU19236.1"
                     /translation="MHLLHPLRARYESRLKKFMRNLRLKPRTLILAALAACTLAAGCR
                     KEIAPGPDVWATVNGKQILRQNVQKIFRSRVTSEAPAPSQEEALSLQLSILDELINSE
                     ILLERANKMNLVASDAEVEDKFTESKSPYTEEEFQKKLQQTGLSVDDLRSDIRRQLSI
                     QKLLNREVVAKISITDQDITDFYNKNRSQFNVAEPQYHIAQIVITPRPDPTIHNRKND
                     KATNEAEAGRKAAMLIQRLDAGADFSETAMDYSEDPSASTGGDLGFQPESSFNKSDPV
                     LKKAVLSLKPGQFTQPIRVKDGGYIILKLLAKEAAGQRELSDVQVQQAIRDALRTRKE
                     QLLRSAYLVEARDESHVTNYLAREILESAGKFPDTDAK"
     gene            complement(2683..2874)
                     /gene="rpmB"
                     /locus_tag="VN788_01500"
     CDS             complement(2683..2874)
                     /gene="rpmB"
                     /locus_tag="VN788_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HXU19237.1"
                     /translation="MALACEICGKKPRYGNTISHAHNVTRRRWNPNLRRVRAIVNGAR
                     KQVRVCTSCIRSGRVTKAA"
     gene            complement(2968..4356)
                     /locus_tag="VN788_01505"
     CDS             complement(2968..4356)
                     /locus_tag="VN788_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008954583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXU19238.1"
                     /translation="MKCHSILEAVAGTPLIRLNRVAKDVPAEVYVKAEYLNPGGSMKD
                     RIAIHMIDEAERKGLLRPGGTIVEGTSGNTGMGLALVAAVRGYKVVFTINDKQSREKI
                     DLLKAFGAEVIVCPTAVEPENPRSYYSVAKKLAREIPNAYYPNQYENQANPDAHYRAT
                     GPEIWKDTEGKITHFVCGLGTGGTASGVGRYLKEQNPNVKVIGVDPYGSLYYEYWKTG
                     NIGKARTYVVEGIGEDILPSTMDFSVLDDIVQVNDEECFLWARRLAKREGIFTGGSGG
                     GCVSAALRVAKQCQKGDLIVGLLPDSGTRYLSKIYNDGWMRELGYADTEVTLTAADCI
                     RVKRQNGKTRDLIVVGPNRTIFQALQTMQQQDISQLPVFEDDRSIGTIFEDQILNLAL
                     QGKELRKLIVREVMGSALPQVAANAPVERVTHLLSHESPAVFVEMEAGNLEVLTKFDL
                     MDTIAGLVGQKR"
     gene            4479..>4901
                     /locus_tag="VN788_01510"
     CDS             4479..>4901
                     /locus_tag="VN788_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DCC1-like thiol-disulfide oxidoreductase family
                     protein"
                     /protein_id="HXU19239.1"
                     /translation="MGACYAFGSVPRMAPDAEIIFYDGHCGLCDRAVKFVLKHDRSGR
                     AFRFAPLQGTTFASLVSAEQRAKLPNTIVVLTEDGATLTRSDAFVHIFRRLGGGWRTL
                     AGALAIIPPPIRDLIYDFIARIRYRIFGRRDELCPIMPP"
BASE COUNT          963 a         1371 c         1414 g         1153 t
ORIGIN      
        1 cttgccgaga tggtactttt ttacgcaatg ccgcgctgga cattgtgttg cccttcaggt
       61 aacatcgcgc cgttttatat tccttcgctc ttaaaaaaag gggaaaattc taaaatagca
      121 atgcctaaga accgcgcaaa aacagcgact atgctcgaat cgcttgatgc tttgatagag
      181 cagtatcacc ttctcaagca tcctttctac caagcttgga cgaaaggcac gctttctcgc
      241 gaatctcttc aactttacgc ggagcagtat tatcaacacg tccgcgcctt tccggaaaac
      301 ttgaagcagt tcgccgatcg ggcggaagag cctctcgccg gactcgtaga ggataatctc
      361 gccgaagaac tcgatccggt atctccgcat ccagctctct ggcggcaatt tgccgaatcg
      421 ctcggcgtaa gcgaagcctc gctcgaggaa gcgcatccgc ttcccggcat cgcggcgttg
      481 ctcgattcat acgacgaggt cgtgtctcag ggaacgatgg cgcaggctgt ggcagcgttc
      541 tatgtttatg aggcgcaggt gcctgaaatt gcgacgcaga aaatttcggg gctgaagcgc
      601 ttttacgaca tctccgagcc tcgtgctctg gcctactttg gcgttcacga agaagctgat
      661 gtgcgtcacc gtgcggcctg gcgccaatgg ctcgccagcc aaaacgagga cgatagcttg
      721 ggcgtgctct gtgtggcaga gcgcagcctg aaggcactat ggggcgctct ggatgcggtt
      781 tatccagagg tttgcgccaa gaactaatgg ccagcgctca gctggtttaa attcggcgac
      841 aggaccggag cctagttgga ttggtaccgc gctctggtcc tcgccaaatc tcttcgtcac
      901 ttatccgatc tttttacgat cttcgcgccg tcacttccgc cgacgatcac ctctcgcgcg
      961 aatcccagga acaggccgtg ttcaacggca ccgatcacgt gatcgagatg gtgcgccgtt
     1021 tccttggggt tatccatcgg cccgaaagcg caatccgcga tgtagttgcc gccgtcggtg
     1081 atcagaggtt tgccgtcccc accgagtctt agctccgaag ttccggccag ctcaccgagt
     1141 tttttcagag ttacctgcca gccgaattga acgatttcca cgggcaccga agcgagagtg
     1201 ccgagctttc ccgagacttt cgtttcgtcc gcgacaacta tcatccggtc gctcgccgcc
     1261 gcgacaattt tctcgcgcag cagcgcgcca ccatgacctt taatcagaga gagcgtgctg
     1321 agctcgactt cgtccgcgcc atcgatcgta agatcaattc gagtacgctc ggcaaaattg
     1381 atcagcggga tttttagctc gcgagcaagc gcagcggtgc gcgccgaagt ggggattccg
     1441 gaaatgcgca agcgttccga cgagagccgc tgcgctaaag ctgtgaggaa gaattccgcc
     1501 gtggaaccgg tgccgaggcc gactgccatt ccatcttcaa cgagccgcgc ggcagattcc
     1561 gcggctacct tcttccattc attttgcatc gaagcatcaa tcggcattct tatttcgcgt
     1621 cagtgtcggg aaattttccg gcggattcga ggatctcacg ggccagataa ttggtcacat
     1681 gagattcgtc gcgcgcttcc accaaataag ctgagcggag aagctgttcc ttgcgggtgc
     1741 gcaaggcgtc ccggatggct tgttgtacct gaacgtcgga tagttcgcgc tgaccagccg
     1801 cttccttcgc caagagcttg aggatgatgt agccgccatc cttcacgcga attggctggg
     1861 tgaactggcc gggcttgagg gagagcacgg ctttcttgag aacgggatcg gatttattga
     1921 atgacgattc cggctgaaac ccgaggtcgc cgccggtgga ggcgctgggg tcctccgaat
     1981 aatccatcgc cgtctcggaa aaatcagcgc cggcgtcgag tcgctggatc agcatggcgg
     2041 ctttgcgacc tgcttcggct tcatttgtgg ctttgtcgtt ttttcgatta tgaatggtcg
     2101 gatcgggccg cggggtgatt acaatttgcg cgatgtggta ctgcggctcg gcgacattga
     2161 attgcgagcg gttcttgttg tagaagtccg tgatgtcctg atcggtgatg gaaattttcg
     2221 cgacgacttc gcgattcagg agcttctgaa tggagagctg acggcgaata tcgcttctca
     2281 gatcgtccac actcagcccg gtttgttgca gctttttctg aaattcctct tcggtgtaag
     2341 ggctcttgga ttcggtaaat ttatcctcga cttcggcgtc gctggcaacc agattcattt
     2401 tgttggcgcg ctcgagaaga atttcgctgt tgatcagctc gtcgagaatg ctgagctgca
     2461 gcgatagcgc ctcctcctgc gacggggctg gggcttccga ggtaactcgc gagcgaaaga
     2521 tcttctggac attctggcga agaatttgct tgccgttcac cgtggcccac acgtcggggc
     2581 caggcgcaat ttctttcctg catccggcgg cgagcgtgca agcggcgagc gccgcgagaa
     2641 tcagagtcct cggcttcaac cggaggttcc gcatgaattt ttttaggcgg ctttcgtaac
     2701 gcgcccggag cggatgcagg aggtgcatac gcgcacctgc ttgcgggcgc cgttgacgat
     2761 ggcgcggacg cggcgcagat tgggattcca gcggcggcgc gtaacgttgt gcgcgtggct
     2821 gatggtattg ccgtagcgcg gcttcttgcc gcaaatttca caagcgagtg ccatgtttcc
     2881 gttgctccag gctggaagga tttccgaaga atgcgaatcc tgatgctacc agagaatggc
     2941 acgcgataga agcgatttgc agcgagatca ccgcttctgg ccgactaagc cagcgatcgt
     3001 atccatcaga tcaaatttgg tgaggacttc gaggttgccc gcttccattt cgacgaaaac
     3061 ggcggggctt tcgtggctga gcaggtgagt gacgcgttca actggtgcat tggcggcaac
     3121 ttgcggcagg gccgacccca ttacctcgcg gacaatcagc ttgcgcaatt ccttcccctg
     3181 caacgcgaga ttcaggattt gatcttcgaa gattgtgccg atggaacggt cgtcctcgaa
     3241 aacgggaagc tgggaaatat cttgctgctg catggtttgg agcgcttgaa atatggtgcg
     3301 attcgggccg acgacgataa gatcgcgcgt cttgccgttc tggcgcttca cgcggataca
     3361 atcggcagcg gtgagtgtga cctcggtatc cgcatagccg agctcgcgca tccagccgtc
     3421 gttatagatt ttgctgaggt agcgcgtgcc agaatctggc agcagtccaa caatcagatc
     3481 gcccttttgg cactgcttgg ccacgcggag cgccgcggaa acgcagccgc cccccgagcc
     3541 accggtgaaa ataccttcgc gtttggccaa ccgccgcgcc cacagaaagc attcttcatc
     3601 atttacctga acgatatcgt ccagcaccga aaaatccatt gttgacggga ggatgtcctc
     3661 cccaattccc tccaccacgt acgtgcgggc cttgccgatg ttgcccgttt tccaatattc
     3721 gtaatagagc gaaccatatg gatcgacgcc aatcactttc acgttgggat tctgttcctt
     3781 gaggtaacgc cctacgccgc tcgccgttcc gccggtgccc aggccgcaaa caaaatgtgt
     3841 aattttgcct tcggtgtcct tccagatttc ggggccggtg gcgcgatagt gggcgtcggg
     3901 gttagcttga ttctcatatt ggtttgggta gtaagcgttg ggaatttcgc gcgcgagctt
     3961 ttttgccacc gaataatagc tgcgcggatt ttcaggttcg accgccgtgg gacatacgat
     4021 tacttccgca ccgaaagcct tcagcagatc gattttttcg cgcgactgct tatcattgat
     4081 ggtgaagact accttgtagc ctcgcacggc cgccacgagc gcaagcccca tgcccgtatt
     4141 gcccgacgtg ccttcgacga ttgtgccgcc ggggcgcagc aggccttttc tctccgcttc
     4201 atcaatcatg tggatggcga tgcggtcctt catggaaccg cccggattga gatactcagc
     4261 cttgacatac acttcggcgg gcacatcctt ggccacgcga ttcaaacgaa ttagcggcgt
     4321 accggcgacg gcctcaagga tgctgtggca cttcacgact gcgcgtgctc ctgtcgcatt
     4381 ggcgactcgc gtgtagctta gaggcttcgc gaaagggtgt ccagcggcag tttgtgaatg
     4441 gcggaggacg attcgaccga gcagcgagac cacaagcgtt gggcgcgtgc tatgcttttg
     4501 gcagcgtgcc gcgcatggcg cccgatgccg aaatcatttt ctacgacggc cactgtggat
     4561 tatgcgaccg ggccgtgaaa ttcgtgctca agcacgaccg atcaggccgc gcttttcgat
     4621 tcgcgccact gcagggcacc acctttgcat cgctggtttc cgccgagcag cgcgccaagt
     4681 taccgaacac catcgtcgtg ctcacggaag atggcgctac cctcacgcgc tcggatgcat
     4741 tcgttcacat atttcggcgg ctgggcggag gctggagaac tttggcgggg gcgctggcga
     4801 ttattccgcc gccgattcgc gatttgatct acgatttcat cgcgcgaatt cgctaccgca
     4861 tatttggccg gcgcgacgaa ctatgtccga tcatgccgcc t
//
LOCUS       DATNDT010000046         2627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3395118, whole genome shotgun sequence.
ACCESSION   DATNDT010000046 DATNDT010000000
VERSION     DATNDT010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3395118"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1104)
                     /locus_tag="VN788_01515"
     CDS             complement(<1..1104)
                     /locus_tag="VN788_01515"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXU19240.1"
                     /translation="MKIKNICWLPSVAIFAGLIAAPSICLAQTKGTVVEQIVARVNNE
                     IITMSDYEKASAGLAQEVRQGCQNCPAEQIQSQLKERQKNLLRDMIDQQLLIARAKDM
                     DINVETDLVKRLDDVRKQNNLATVDDLEKAVEAQGIVWEDYKNQLRNSILTQQVIQRE
                     VGGRMDIGSDEVKKYYDAHKQDFVRPEEVSLAEIMLSTEGKSPEEISAVRTRAEDLHN
                     RLVKGEDFNVLAKRYSEGSTAQDNGVLGEFQRGQLAKQFEDAVFPLEKGQFTDVIQTK
                     TGFEILKVLDHYQAGLQPLSKVENEVTNILYRQKMDPAMRTYLAQLREESYVQVKPGY
                     TDTAAVAGGTVIQEVAPTPDTSSKKKEKKKLPLP"
     gene            complement(1130..>2627)
                     /locus_tag="VN788_01520"
     CDS             complement(1130..>2627)
                     /locus_tag="VN788_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TRCF domain-containing protein"
                     /protein_id="HXU19241.1"
                     /translation="AAFKALADSKQVAVLAPTTVLAFQHLETFRRRFAAFPARIEMLS
                     RFRTAKEQKKALEELEAGKVDVIIGTHRLLSKDVKFHDLGLLIVDEEQRFGVAHKERL
                     KEIRKNVDVLTMSATPIPRTLHMSLVGLRDMSVIETPPRDRLAIQTSVSPFSETLVQR
                     VVEEELSRGGQVFFVHNRVESIASLASMVQRLVPRARVVVGHGQMHESSLEKVMLKFI
                     RNEANVLVSTTIVENGLDIPRANTILINRADRMGLSELYQLRGRVGRSNQRAYAYLLI
                     PPEGSLTPIARQRLAALQEFSDLGAGFRIAALDLELRGAGNLLGGEQHGHIEAVGFDM
                     YCQMMERAVAERKGEPVAPERRATLNLGQDIRIPAEYIENENLRLRIYKRIAGVTSEA
                     SRDEVRKELDDRFGPPPPAVVNLLDYAVLKALAEKLLVANIDRRGDQLAIKFYDDTPL
                     GPERLVRLIGKRRDMRLDPAGMLLLDWKRSKETSPMEAAKKVLMQLQS"
BASE COUNT          506 a          706 c          798 g          617 t
ORIGIN      
        1 cggcagagga agcttctttt tttctttttt cttgcttgac gtatccggcg tcggtgcgac
       61 ctcctggatg acggttccgc cggcaacggc ggccgtgtcc gtataaccgg gtttcacctg
      121 cacgtaactt tcctcacgca attgcgcgag ataggtgcgc atagcggggt ccattttttg
      181 ccgatagaga atgttggtaa cttcgttttc gaccttgctg agcggctgca agcctgcctg
      241 ataatggtcg agcaccttga ggatttcaaa accggttttg gtttgaatca cgtcggtgaa
      301 ctgacccttt tccagcggga agacggcatc ttcgaactgc ttggccagct ggccgcgctg
      361 aaattcgcca agaacgccgt tatcctgggc cgtggagccc tccgaatagc gtttcgcgag
      421 cacattaaaa tcttcaccct tcaccaagcg gttgtggaga tcttcggcgc gcgttcggac
      481 ggcggagatt tcctcgggac ttttgccttc cgtgctcagc atgatttccg caagggacac
      541 ttcttcgggg cggacaaaat cctgtttgtg cgcgtcgtag tatttcttca cctcgtccga
      601 gccaatatcc atgcggccgc ccacttcgcg ctggataacc tgctgggtca agatgctgtt
      661 gcgcaactgg ttcttgtagt cttcccagac gatgccctgt gcctcgacgg ccttctcaag
      721 atcatcgacg gttgcgagat tattctgctt gcggacgtcg tcgagccgct tgaccaggtc
      781 tgtctcgaca ttgatatcca tgtccttcgc gcgcgcgatg aggagctgct ggtcaatcat
      841 gtctcgcagc aggttcttct ggcgttcctt cagttgggat tgaatttgct cagccggaca
      901 attctggcaa ccctgccgga cttcctgagc gagtccggcg gaagcctttt cgtaatcgga
      961 catggtgatg atctcgttgt tcacgcgggc gacgatttgt tcgacgacgg tgcccttggt
     1021 ctgagcgagg cagatggaag gagccgcgat caggccggcg aaaatagcga cggatggaag
     1081 ccaacaaata tttttaattt tcatggtaat cactctggcg attttatcgc tacgattgta
     1141 gttgcatcaa cactttcttc gcagcctcca tggggcttgt ttccttgctt cgcttccaat
     1201 cgagcaggag catgccggcg ggatcgaggc gcatgtcccg gcgcttgccg attagcctca
     1261 cgaggcgttc cgggccgagc ggtgtatcgt cgtagaactt gatggccaac tgatcgccgc
     1321 ggcgatcgat attggcgacg agaagttttt ccgccaaggc tttcagcaca gcgtagtcga
     1381 gcaagttcac cactgcaggc ggcggaggac caaagcggtc gtccaattcc ttgcgaactt
     1441 catcgcgcga tgcctcggac gtgaccccag cgatgcgctt gtaaattcgc aggcggagat
     1501 tctcattttc aatgtattcc gcggggatgc ggatgtcttg accgagattc aaagtggcgc
     1561 ggcgctcggg cgcgacgggt tcgccctttc tttcggctac ggcgcgctcc atcatctggc
     1621 aatacatatc gaaaccgacg gcttcgatgt ggccgtgctg ctcgccgccg aggagattac
     1681 cggctccgcg caattcgaga tcaagagcgg cgatgcgaaa gcccgcgccc agatcactga
     1741 actcctggag agcggctagg cgctggcgcg cgatgggagt aagcgaacct tctggcggaa
     1801 tcaaaagata ggcgtaggcg cgctggttgg agcggcccac gcgtccgcga agctgataca
     1861 gctcggaaag acccatgcgg tccgcgcgat taatgaggat ggtattggcg cgaggaatgt
     1921 cgaggccgtt ttcgacgatg gtggtggaaa cgaggacgtt ggcttcattt cggatgaatt
     1981 tcagcatcac tttttcaagc gacgattcgt gcatctgacc gtggccgaca acgacgcgcg
     2041 cgcgcgggac gaggcgctgc accatcgatg cgagcgaggc gatcgattcg acgcgattgt
     2101 ggacgaagaa aacctggccg ccgcgcgaga gttcctcctc aaccacgcgc tgcaccagag
     2161 tttcgctgaa aggcgagacg ctggtttgaa tggcaaggcg atcgcgaggc ggtgtttcga
     2221 tcacgctcat atcgcgcaag ccgactagcg acatgtggag agtgcgcggg atcggagtgg
     2281 cggacatggt gagcacgtcc acattcttgc ggatttcctt gaggcgctct ttgtgcgcca
     2341 cgccgaagcg ctgctcttcg tccactatga ggaggccgag gtcgtgaaac ttcacgtcct
     2401 tggaaagcag gcgatgcgtt ccgatgatta cgtccacttt gccggcttcg agttcctcga
     2461 gggctttttt ctgttccttg gcggtccgaa agcggctgag catttcgatg cgcgcgggaa
     2521 aagcagcgaa gcggcggcga aatgtttcca gatgttgaaa ggctagaacg gtggtgggcg
     2581 cgaggacggc gacctgcttt gagtccgcaa gagctttgaa agcggcg
//
LOCUS       DATNDT010000047         6144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3650303, whole genome shotgun sequence.
ACCESSION   DATNDT010000047 DATNDT010000000
VERSION     DATNDT010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6144
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3650303"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1852
                     /locus_tag="VN788_01525"
     CDS             <1..1852
                     /locus_tag="VN788_01525"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HXU19242.1"
                     /translation="RASWATFDKLRDHNDSLLHGILEEASKDTKAQPGSNWQKIGDFY
                     ASCMDESQVETAGLKPIQTELQRIAAINDIASLQDEIARLQHSGANAVFAFGSEQDLK
                     DSTQVIAGAGQGGLGLPDRQYYVDDDARSKQLRAAYIQHVTNMFKLMGDDPSTSAAEA
                     NSVMAAETALATASKKREDLRDPETNYHPMTLVQLRDLTPHFSWATYFKEVGAPPLAS
                     ADIGQPDFFKQVDASLTTISLDDWKTYLRWHLIHSVAASLPQKFVEENFNFYGRTLTG
                     AKQMLPRWQRCVQATNRELGEALGQYYVQRYFPPEAKAHALVMVHNLMDALRADLSTL
                     DWMSPATREQAIKKLDAMNLKIGYPDKWRDYSAYKVDRGPYVENVLRGNSFEFARDIA
                     KIGKPVDRTEWGMTPPTVNAYYNPSMNEIVFPAGILQPPFFDPHADDAINYGGIGSVI
                     GHEMTHGFDDQGAKFDGQGNLKNWWTPQDLKNFQARGECIVNQFGSYEIEPGLHGNGK
                     LEEGESIADLGGLTISHAAFEKTLAGKPAPPPIDGFTAEQRFFLGWAQIWAANLRPEY
                     ARLLAKTNPHPLDKFRGNGAPSNTPAFAKAFGCAADSKMIRPPQQRCRIW"
     gene            2006..2833
                     /locus_tag="VN788_01530"
     CDS             2006..2833
                     /locus_tag="VN788_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19243.1"
                     /translation="MKERQKSLELALEEAVSRALRDNLAPLQRTADELHQAYSDLVAA
                     CSSTRPSNALPAMLRAQTAASALAAGLSVLSNFVAVALNPREALYSDDLPLSTESAVA
                     VQEPANIPDVPHVPHLEGAAPAAIAEDAESEWAEAAEEVQDIPVSTPENSPAAAPPST
                     VTAEPPATAVPAFDVASLTPDLQDLHRRANRVAKVAMQDIKLLRPKDVRLGRENKDIC
                     IRLRSDLDKARKEYDRRFRAIQDHPVDYFQRWMVEILAEGDVATLGEYPYPSPVLRH"
     gene            2844..5075
                     /locus_tag="VN788_01535"
     CDS             2844..5075
                     /locus_tag="VN788_01535"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HXU19244.1"
                     /translation="MKFAPRLVCLGASLLIVCSLCSAGTVARPAAATRVVLTQKKLEK
                     LSKDLKGKGKKASSAYAGLSWLARNSSGPLSARAALALGYYDYGKENYTQAQIWFARG
                     KEDPLLSDYALYWVAETDIAEQRNADALAELQKFRAQFPDSAMTEQALQSLGNAAIAA
                     KQPQAAVAALDAYDLTPQRPALLFLRGEAKEQAGKPADAANDYAALYLRFPFSEHARE
                     AWEKLNFLRSTLGPAFPALPLEKQLDHASILFSNQNYIDARNEYSAILSQLSGAVRER
                     AQLRILECGLALGDPPTEVSALVVTDPDVDAERSYALANYYRGLQQEPQMISAVESAV
                     SRAPSSNWTEAALFLAGNFYWVQLARDQASSYYKRVEEQFPDSADADNAHWRVTWTAV
                     LKRDPDGAKLLQEHLRRFPGSQYTPDALYWLGRLAEEAKTPALARDYYRKLAQRYPRN
                     YFESLASKRLRTLGPGPVEVSDVEVSNLLATIPPVSAAPKLDGAIPPGAAKLKARADA
                     LRSIAFDASAELELRAAYAATGSARLLLESAQAAVAAGQYGAAIVTVRKIFPHLDGQS
                     FSSVPRNVWMAAYALPYKDLIQHWSSRTALDPMLVAGLIHQESAFQMDATSVSNALGL
                     MQLLPSTARRLARQSRLRYSESRLFNPAYNVRLGTTYLANLHKQFGSVEEVLAAYNAG
                     EDRVTAWTAGQNYRETAEFVESIPFTQTRQYVEIISRNAAIYRRLYGAQHESPTDPMG
                     TGN"
     gene            5038..>6144
                     /locus_tag="VN788_01540"
     CDS             5038..>6144
                     /locus_tag="VN788_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007950241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXU19245.1"
                     /translation="MNPPPIQWVPATSPALSSKAGRFTESVIREMTRLALRHQAVNLS
                     QGFPDFPAPAEIKAAAQQAIAADVNQYAITWGSKSLRNAIAENFERTRGMAIDPETEV
                     TVCCGSTEAMISSMMAIINPGDEVVVFEPFYENYGPDAILSGAVPRYVKLRPPGWTFD
                     PEELAAAFSPATKAIIVNTPNNPTGKVFERAELECIRDLCVQWNCFAITDEIYEHMLY
                     DGAKHISLATLDGMRERTITINALSKTYSVTGWRVGWAIASAEVTGAIRKVHDFITVG
                     AAAPLQEAGAVALQFPMSYYETLARDYAVRRDRLLGILTNAGFRCFKPRGAYYIMTDI
                     SAFGFPDDVAFAKHLVTQIGVAAVPGSSFYRDPAD"
BASE COUNT         1283 a         2029 c         1578 g         1254 t
ORIGIN      
        1 tcgcgcttcc tgggccactt tcgacaaact ccgcgatcac aacgattccc tcctccatgg
       61 aattctcgag gaagcttcca aggacaccaa agcccagccc ggctccaact ggcaaaagat
      121 cggcgatttc tatgcaagct gtatggatga gagtcaggtc gaaactgccg gactcaaacc
      181 catccagacc gaattgcagc gtattgccgc gatcaatgac atcgcctctc tccaggatga
      241 aatcgcgcgc ctccagcaca gcggcgccaa tgccgttttc gcatttggtt ccgaacaaga
      301 tctaaaagac agcacccagg taatcgcagg ggccggccaa ggcggactcg gcctccccga
      361 ccgccagtat tacgtcgatg acgatgcccg ctcaaaacag cttcgcgccg cctacatcca
      421 gcacgttacc aatatgttca agctgatggg tgacgatccc tccacctccg ccgccgaagc
      481 caattccgtg atggctgccg aaactgcgct tgccaccgcc tccaagaagc gcgaggattt
      541 gcgtgatccg gaaacgaatt atcatccaat gacgttggtc caactccgcg atctcacccc
      601 tcacttctcc tgggccactt atttcaaaga agttggcgcg ccacctctcg ccagtgccga
      661 tatcgggcag cccgacttct tcaagcaagt ggacgcaagt ctcaccacca tttccctcga
      721 cgactggaag acttacctcc gctggcacct aatccattct gtagccgcga gcttgcccca
      781 aaaattcgtc gaggaaaatt ttaactttta cggtcgcacc ctcaccggtg ccaagcagat
      841 gcttcctcgc tggcagcgat gcgtgcaggc caccaaccgc gagcttggcg aagccctcgg
      901 acaatattac gtccagcgct attttccgcc cgaagcaaaa gcccacgcac tcgtgatggt
      961 ccacaatctg atggatgccc tccgcgccga tctctccacg ctggattgga tgagccctgc
     1021 cactcgcgag caagccatca aaaaactgga cgccatgaat ctcaaaatcg gctatccgga
     1081 taaatggcgc gattactccg cctacaaagt tgaccgcgga ccctacgtgg aaaatgtcct
     1141 tcgtggcaac tctttcgagt ttgcgcgcga catcgccaaa atcggcaagc ccgttgaccg
     1201 caccgaatgg ggcatgacgc cgccaaccgt caatgcctat tacaatccct ccatgaatga
     1261 aatcgttttc cccgctggaa tcctgcagcc tcccttcttc gatccccacg ccgacgatgc
     1321 catcaactac ggcggcatag gctcggtcat cggccatgaa atgacgcacg gcttcgacga
     1381 ccagggagcg aaattcgatg gtcagggcaa cctgaagaat tggtggacac cccaggacct
     1441 gaaaaatttc caggctcgcg gtgagtgcat cgtcaatcag tttgggtcct acgaaatcga
     1501 acccggcttg cacggaaacg gtaagctgga agaaggagag agcatcgccg atctcggcgg
     1561 cctaaccatt tctcacgccg cgttcgagaa aactcttgct ggcaagcccg ctcccccacc
     1621 tattgatggt ttcaccgcgg agcagcgctt cttcctgggc tgggctcaaa tttgggctgc
     1681 caacctgcgc cccgaatacg cccgcctcct cgccaagacg aatccgcatc cgctcgataa
     1741 attccgcggc aatggcgcac cctcaaacac tcccgcgttc gccaaagcct tcggctgcgc
     1801 tgccgattcg aagatgattc gtcctccgca acagcgctgc cgcatctggt gacgttccat
     1861 gtagcatctc ggctacccca ggcggcacac aaatcttccg cagtgccaat tcgaccagcc
     1921 tcgcatgctt ggcaggaact cgccgcccga atgccagttg acactcctcc gcgccgtttc
     1981 cataattcaa tatgcgggta ttcccttgaa agaacgtcag aaatcgctcg aacttgccct
     2041 cgaagaggct gtctccaggg ccttgcgcga caatttggct ccgctccagc ggactgccga
     2101 cgagttgcat caggcctatt ccgacctcgt cgcagcctgt tcgtccactc ggcccagcaa
     2161 cgcgcttcct gcaatgctgc gagcgcaaac tgctgcctcg gcgctagccg cgggtttgtc
     2221 cgtcctctcc aacttcgtcg ccgttgcctt gaatccgcgt gaggccctgt attctgacga
     2281 tctcccgctc tcgacggaat ccgccgtcgc cgttcaggag ccggcgaata ttcccgatgt
     2341 gccccacgtg ccccatctag aaggtgccgc gcccgctgct attgccgagg acgccgaatc
     2401 cgagtgggcc gaggccgccg aagaagttca agacatcccg gtaagcacgc cggaaaattc
     2461 cccggcagcc gctccgccgt caaccgtcac cgccgaacca ccggccactg ctgtgcccgc
     2521 gtttgatgtt gcgagtctca cgcccgattt acaagatctc catcgccgcg cgaatcgcgt
     2581 cgccaaagtg gccatgcaag atatcaaact attgcggccc aaagatgttc gcttgggccg
     2641 ggaaaataaa gacatatgca ttcggttgcg cagtgatctc gataaagccc gcaaagaata
     2701 cgaccgccgc ttccgcgcga ttcaggatca cccggtggat tatttccagc gttggatggt
     2761 ggaaattctc gcagagggag atgttgcaac tctcggcgaa tatccctacc cgtcgcccgt
     2821 cctgcggcat tagatccaac gcgatgaaat tcgcgccacg cttggtgtgc ctgggcgctt
     2881 ccctgctgat cgtgtgctcg ctttgctccg ctggcaccgt cgctcggccc gctgctgcca
     2941 ctcgcgtggt cttaacccaa aaaaagctcg aaaaactttc caaggatctc aaaggtaagg
     3001 gcaagaaagc ctcctcggcc tatgccggcc taagctggct cgcgaggaac tcttccggtc
     3061 cgctttcggc gcgtgcggcg ctggccctgg gctactacga ttacggcaag gaaaactaca
     3121 cccaggcgca aatctggttc gcacgcggga aggaggatcc ccttctttcc gactacgcgc
     3181 tgtattgggt tgcggaaacg gatatcgccg agcagcgcaa tgccgacgcg ctcgctgaac
     3241 tccagaaatt tcgcgcgcag tttcccgact ccgcgatgac ggaacaagcg ctccagtctc
     3301 tcggcaacgc cgcgattgca gcaaaacagc cgcaggccgc cgtggctgcc ctggacgcct
     3361 acgatcttac tcctcagcgt cccgctcttt tgtttttgcg tggtgaagcc aaggaacaag
     3421 cgggcaaacc agccgatgcc gccaacgatt acgcggctct gtacctgcgc tttcccttca
     3481 gtgagcatgc tcgcgaggcc tgggagaaac tcaatttcct gcgcagcacc cttggccccg
     3541 ctttcccggc ccttccgctc gaaaagcagc tcgatcacgc ctcgatcctt ttcagcaacc
     3601 agaattacat cgacgcgcgc aacgaatatt ccgcgatttt gtcgcagctt tccggtgccg
     3661 tccgcgaacg ggctcaattg cggattctcg aatgcgggct agccttgggc gaccctccca
     3721 cggaagtttc cgccttggtc gtcaccgatc ctgacgtcga tgcggagcgc tcctacgctc
     3781 ttgccaatta ttatcgggga ctccagcaag agcctcagat gatctcggcc gtcgagtccg
     3841 cggtttctcg cgccccctcg agcaactgga ccgaagccgc tctttttctc gccggaaact
     3901 tctactgggt gcagctcgcc cgcgatcagg cgtccagcta ttacaaaaga gttgaggaac
     3961 agtttccgga ttcagccgac gcggataacg ctcattggcg cgtcacttgg actgcggtcc
     4021 tgaagcgcga tccagacgga gcgaaactgc tccaggaaca tctgcgccgc tttcccggct
     4081 cccagtacac cccagacgct ctctattggc ttggccgcct tgcagaagag gctaaaactc
     4141 cggcactcgc gagggactac tatcgaaaat tagcgcagcg ctacccgcgg aattatttcg
     4201 agagcctggc ctccaagcgt cttcgtactc tgggccctgg tccggtagaa gtttctgatg
     4261 tggaagtatc caatttgttg gcgacgattc ctccggtgtc tgcggcgccc aaattagatg
     4321 gagccatccc gcccggcgcg gcaaagctca aggcgcgggc ggacgcgctg cgctccatcg
     4381 ccttcgatgc ttccgccgaa ctcgaattgc gcgccgcata cgcggccacc ggatcggcgc
     4441 gtcttctgtt ggaatcagct caggccgcgg tcgccgctgg acaatatggc gccgccatcg
     4501 taaccgtccg aaaaattttc ccgcacctcg atggccagtc tttttccagc gtgccgcgca
     4561 acgtgtggat ggccgcctat gccttgccgt acaaggattt gatccaacac tggtcgagcc
     4621 gaaccgctct tgatcccatg ctcgtggccg gattgattca tcaggagtcg gcctttcaga
     4681 tggatgccac ttccgtcagc aatgctcttg gcctgatgca gttgcttcct tcaaccgccc
     4741 gtcgtctcgc gcgccagtca aggcttcgat attcggagtc gcgcctcttc aatcccgcct
     4801 acaatgtgcg gttgggaact acttacctcg ccaatcttca caagcagttc ggcagtgtgg
     4861 aagaggtgct ggccgcgtac aatgctgggg aagaccgcgt gacggcgtgg actgccggcc
     4921 agaactaccg cgaaaccgcc gaattcgtcg agtcgattcc atttacgcag acgcgccagt
     4981 acgtggaaat catctctcgc aacgcggcca tctatcgcag gctctacggg gcacagcatg
     5041 aatcccccac cgatccaatg ggtaccggca actagcccgg ctctttcctc caaggccggc
     5101 cgtttcaccg aatcggtgat ccgcgaaatg acacgcttgg cattgcgcca ccaggccgtc
     5161 aacctctcgc aaggattccc ggacttcccc gccccggctg aaattaaagc tgctgcccag
     5221 caagcaattg ccgccgacgt gaatcagtac gccattacct ggggctcaaa atcgctgcgc
     5281 aatgccatcg ccgaaaactt tgagcgcacg cggggaatgg cgatcgatcc ggagaccgaa
     5341 gttacggttt gctgcggctc tacggaagcg atgatctcgt cgatgatggc catcatcaat
     5401 cccggcgacg aagtagtagt cttcgaaccc ttctacgaaa attacggccc ggatgcgatc
     5461 ctttccggcg ccgtccctcg ctatgtcaag ctacgtccac ccgggtggac cttcgatccc
     5521 gaagaactgg cggcggcctt cagtccggcc accaaagcaa tcattgtgaa cactccgaat
     5581 aaccccactg gcaaagtatt cgaacgcgcc gaactcgaat gcatccgcga tctctgcgtc
     5641 cagtggaact gtttcgccat caccgacgaa atttacgagc acatgctcta tgacggcgcc
     5701 aagcacattt ctctcgccac cctcgatggc atgcgcgaac gcaccattac catcaacgcc
     5761 ctatcgaaaa cgtatagcgt caccggctgg cgggtaggct gggccatcgc ttccgcggaa
     5821 gtcaccggtg cgatccgcaa agttcacgac ttcatcaccg tcggcgcggc cgcgcccttg
     5881 caggaagcgg gcgcagtcgc cttgcaattt cctatgagct attacgaaac gcttgcgcgc
     5941 gattacgccg tccgccgcga tcgcctgctc ggcatcctca ccaacgccgg attccgttgt
     6001 ttcaaaccgc gcggcgccta ctacatcatg acggacatct ccgcattcgg atttcccgac
     6061 gacgtagcct tcgcaaagca tctcgtaact caaatcggcg tcgccgccgt ccccggctcc
     6121 agcttttacc gcgatcccgc cgac
//
LOCUS       DATNDT010000048         4053 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1904440, whole genome shotgun sequence.
ACCESSION   DATNDT010000048 DATNDT010000000
VERSION     DATNDT010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4053
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1904440"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VN788_01545"
     CDS             <1..535
                     /locus_tag="VN788_01545"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU19246.1"
                     /translation="AVLKSLTDSREQLHRMGAGLSREQWHYRAEPGRWSVAECVEHIT
                     TVEARVLDIIEKTLESGPDPSKNNAMTDDALVADVAGRVTRFQAPEILTPTGRWPHEE
                     LLNEFDRVRQRTHDFAANTTVDLRQHFFPHRVFGELDLYQWLLLIAAHCDRHRTQSEE
                     VMASAGYPRTSQTSASA"
     gene            complement(554..1645)
                     /gene="recA"
                     /locus_tag="VN788_01550"
     CDS             complement(554..1645)
                     /gene="recA"
                     /locus_tag="VN788_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HXU19247.1"
                     /translation="MNDEKSKNLDLAISQIEKQYGKGSIMRLGNRDVLVPVSVISTGS
                     ISIDAALGVGGFPRGRVIEVYGPESGGKTTLTLHVIAEAQKTGGIAAFIDAEHALDPI
                     YARKLGVDVDNLLVSQPDNGEQALEIAEALIRSGGVDVVVVDSVAALVPKAELEGEMG
                     EPQMGLQARLMSQALRKLTAIVSKSRTCLIFINQIREKIGVMFGNPETTTGGRALKFY
                     SSIRVDIRRIQSIKEGDRVVGSRTRAKVVKNKVAAPFREAEFDIIYGEGVSREGDLLD
                     LGVMKNVLEKSGTWISYKGERLGQGRENARLFLREHTDFRDKIEHDLRKALGLGSHAN
                     THDDKKPAAEPEKPAAKVAVAAGQGRNNR"
     gene            complement(1914..3698)
                     /locus_tag="VN788_01555"
     CDS             complement(1914..3698)
                     /locus_tag="VN788_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HXU19248.1"
                     /translation="MNRKPWAILAAGTGLLCAVALMAASSLVAHRADDVRAQWNALAD
                     AYFSQVYFVFNPTSGTEAGLHQYDPMLEDYSRAGIDRQVAAYRAFEKRVDSFNPAGLS
                     QEESADRDIVLSNIKGTLLELEDIRGWEKNPDNYSDGITASAYYIMNRKFAPTNDRLR
                     SLVAREKLMPAVLQHARANLKNPPHIFTEIALEQLPGDISFFQHDLPSAFTDATDPQV
                     KTDFAKSNAAVVAAMESYEKWLRTVELPVSNGDFAIGADVYSKKLLYDEMVDTPLDKL
                     LAMDQENMKANQAEFARIAKQLDPTKTPQQMLAELQADHTTPDHLLQAFRDTFSSLIA
                     FIRQKHIVTIPSDVRPIVEDTPPFQRATTSAQMDAPGPFEKVAKEAYFNVTLPDPKSP
                     PKEQEELMEEFNIGTVISVAVHEAYPGHYVQFLWLAQAPSKVRKLLGSNTDVEGWAHY
                     CEQMMLDEGYGQPGTGAKDEREAKLIRLGQLSDALLRNARFVVGIQMHRHRMSFDQAV
                     DYFVKEGYQSHAVGEVEAKRGTSDPTYLYYTLGKLEILKLRADVQKKEGAAFSLEKFH
                     DDFLKQGFPPVKIVRQALLGNNSAKPGH"
BASE COUNT          837 a         1161 c         1134 g          921 t
ORIGIN      
        1 agccgttttg aagagtttga ccgattcccg cgaacaactg caccggatgg gagccggcct
       61 ctcgcgcgag caatggcact atcgcgctga gcccggccgc tggtcggtcg cggaatgcgt
      121 ggagcacatc acgaccgtcg aggcacgcgt actcgacatc attgaaaaaa cactagagag
      181 cggtcccgat ccgtcgaaga acaacgcgat gacggacgac gcactggtcg ccgacgtggc
      241 gggtcgcgtg actcgcttcc aggctccgga aattcttact ccgacaggcc gatggccgca
      301 cgaagaatta ttgaatgaat ttgaccgagt ccggcagcgg acgcacgatt ttgcggcaaa
      361 cactacggtt gatttgaggc agcacttctt cccgcatcgc gtattcggag aactggacct
      421 gtatcagtgg ctgctcctga ttgcggcgca ttgcgaccgt catcggacgc agagcgaaga
      481 agtaatggct agcgcggggt atccccgcac gtcacaaacg agcgcatctg cctgacgcga
      541 ttatcgtttt gcgttagcgg ttgttgcggc cttggcctgc cgctactgca accttggccg
      601 cgggcttttc gggctcagct gcgggctttt tgtcgtcgtg ggtgttcgcg tgcgagccga
      661 gaccgagcgc cttgcgaaga tcgtgctcaa ttttgtcgcg gaagtcggtg tgctcgcgca
      721 aaaagaggcg cgcgttttcg cggccctggc cgaggcgctc gcctttgtag gaaatccagg
      781 tgccggattt ttccagcacg tttttcatca cgcctagatc gagcaaatcg ccttcgcgcg
      841 aaacaccttc gccgtagatg atgtcaaatt cggcttcgcg gaagggcgcg gcaactttgt
      901 tcttcaccac tttggcgcga gtgcgcgagc cgacaacgcg gtcgccttcc ttgatggatt
      961 gaatgcgccg gatatcgacg cgaatcgacg aatagaattt cagcgcgcgg ccgccggtgg
     1021 tggtctccgg gttgccaaac atcacgccga ttttttcgcg aatctggttg atgaaaatca
     1081 ggcaagtgcg ggatttcgaa acgatcgccg tgagtttgcg cagcgcctgc gacatcaggc
     1141 gcgcctgcaa ccccatctgc ggctcgccca tttcaccttc gagttcggcc ttgggcacca
     1201 gcgcggcgac ggaatccacg actactacat cgacgccgcc ggagcgaatc agcgcttcgg
     1261 caatttccag ggcttgctcg ccattatcgg gctgcgagac aagcaagttg tccacatcca
     1321 cgccgagttt ccgcgcgtaa atgggatcga gcgcgtgttc ggcgtcgatg aacgcggcga
     1381 tgccgccagt cttttgcgct tcggcaatca cgtgcagggt gagagtggtc ttgccgccag
     1441 attccggtcc gtacacttca atgacgcggc cgcgcgggaa tccgccaacg ccaagagcgg
     1501 cgtcaatcga aatcgagccg gtggaaatca cgctgacggg aacaagcacg tcgcgatttc
     1561 cgagccgcat gatcgagccc ttgccgtact gcttttcaat ttgcgaaatt gccagatcca
     1621 ggtttttaga cttttcgtcg ttcatgattt acctcgaagg gttgtttcgt ttaggtcccg
     1681 ccgcggcggg cggcccgatt ctaacagagt gacgctagac cgcacggctc atttatcgtg
     1741 gggcattata gcatgaggcg aacaaaaagc aaaacctatg tagggttggg gttgcaactc
     1801 tttcttacat cacaaaaaag cacgggacac ctcgctccct cgctctgtca gggcaagcgc
     1861 aacgaaccgc gcaaagcaga cccacggttt tacgggacgt tttgggttgt gatctagtgc
     1921 ccgggcttcg cggagttgtt ccctagcaat gcctgccgca cgatcttcac gggaggaaaa
     1981 ccttgcttca aaaaatcatc gtggaatttt tcgagcgaga atgccgcgcc ttctttcttt
     2041 tggacgtccg ctcgcaattt caatatttcg agctttccca gtgtgtaata caggtaggtt
     2101 ggatcgctcg tcccgcgttt tgcttcgact tccccgacag catgtgactg atagccttcc
     2161 ttcacgaagt agtcgaccgc ctggtcgaag ctcattcggt gccgatgcat ctggatgccg
     2221 accacgaacc gagcattgcg gaggagagca tcagagagct gcccgaggcg aatcaatttc
     2281 gcttcacgct cgtccttcgc acccgtaccc ggctgaccat agccttcgtc gagcatcatc
     2341 tgctcgcaat aatgcgccca accctctaca tctgtgttcg acccaagcag tttccgcacc
     2401 ttcgacggcg cttgagccag ccacaaaaac tggacgtagt gtcccggata cgcttcgtga
     2461 acggctaccg aaatcaccgt gcctatgttg aactcttcca ttagctcttc ttgctctttc
     2521 ggcggagact ttgggtcggg cagggtcacg ttgaaatagg cttctttggc taccttctca
     2581 aacgggcctg gcgcatccat ctgcgcggag gttgttgcgc gctggaatgg cggagtgtcc
     2641 tccacgatcg gcctcacgtc cgaaggaatc gtcacaatat gcttttgccg aataaacgcg
     2701 atcaggctgc taaacgtatc gcgaaatgcc tgtagcagat gatccggcgt cgtgtgatcg
     2761 gcctgtaatt ccgcaagcat ctgttgcggc gtcttggttg ggtcaagctg tttggcgata
     2821 cgtgcaaatt cggcctgatt tgccttcatg ttttcttggt ccatcgcgag cagcttgtcc
     2881 aggggcgtat cgaccatttc atcgtaaaga agcttttttg agtaaacgtc cgcgccaatc
     2941 gcaaaatctc cgttcgagac aggcaattcg accgttcgca gccatttttc gtagctttcc
     3001 atggccgcaa cgacagcagc gttagacttt gcaaaatcgg tcttcacttg cggatcggtc
     3061 gcatccgtga acgctgaggg caaatcgtgc tggaagaaac tgatgtcgcc gggaagttgc
     3121 tcgagcgcaa tctcagtaaa aatgtgggga ggattcttca ggttcgcgcg cgcgtgttgc
     3181 aaaacggctg gcatcaattt ttcacgtgcg accagcgatc gcaagcggtc attcgtgggt
     3241 gcgaacttgc ggttcatgat gtagtatgcg ctggctgtga ttccgtccga atagttgtcc
     3301 ggattctttt cccatccgcg gatatcctct aattcgagca gggtgccctt aatgttcgaa
     3361 agcacgatgt ctcgatcagc cgactcctct tgcgacaggc ccgctggatt gaaactatct
     3421 acgcgcttct caaaagcccg atacgctgca acctgccggt cgattcccgc gcgcgaatag
     3481 tcctcgagca tcgggtcgta ttggtgcagg ccggcctcgg tgccagacgt ggggttgaag
     3541 acgaaataga cctgcgagaa atatgcgtca gccaatgcgt tccactgtgc gcgaacgtca
     3601 tcagcgcggt gcgcgaccaa cgaactcgcc gccataagcg caaccgcaca aagcaagccc
     3661 gtacctgcag caagaatcgc ccacggcttc ctgttcatcc tcgcctcacc ccggacggcg
     3721 cccgcgcgga attggcgctg ccagcaccga agatcgtact tcaaaggcgg atcattccac
     3781 gaacgcacct gggcgtggga cattttcttt ctgcgctgga ggttagaaag tcgacttcgc
     3841 agcttcgtaa gccccgcgaa gtgtccgcca cccagcatgt ttgaccgaat cagcaggtta
     3901 attcctgtgt ggtatcatcc gcccgctctc gaatgccagc cgaaatacgg agcgaaaggg
     3961 ccggcgacca gccaggttac cgattcagaa atgctcgaca atctccggcg tgtcatcgtc
     4021 gattattatc gcaatgaggc cagccctcaa aac
//
LOCUS       DATNDT010000049         4146 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1070705, whole genome shotgun sequence.
ACCESSION   DATNDT010000049 DATNDT010000000
VERSION     DATNDT010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1070705"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VN788_01560"
     CDS             complement(<1..1179)
                     /locus_tag="VN788_01560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19249.1"
                     /translation="MLRGVLVPGLIVLVIAVTFGDGTVMSYADLDQPAIQYNTQTLHD
                     PGTQLNRKIQYGEVHLKFDGPQGYLRSMLDALDVPIQSQLVVFSKTSFQADRINPHNP
                     RSLFFNDSVVVGWVRGGPIIEVAAEDPKQGMIFYKLSQNPAQKVRLSRDVNCLACHVS
                     YASLGVPGTLMRSVFPAPNGATVSKLGQYLTDDRSPFAQRWGGWYVTGSTGSMPHMGN
                     SVVNKAGRLHPMLQGQPRNLESLQGHFDTAAYLSPYSDVVALMVFEHQMHMMNLFTRV
                     GWDVRGSLYRDEMNPDRGHAATARIIRESARELVDYMLFVDEAPMTSRVRGTSGFAEQ
                     FSTEGPTDSQGRSLRQFDLQHRLMRYPCSYMIYSDAFDALPDELKSAVYERMWEILSG
                     Q"
     gene            1432..1713
                     /locus_tag="VN788_01565"
     CDS             1432..1713
                     /locus_tag="VN788_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000035 - acyl binding [Evidence IEA];
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXU19250.1"
                     /translation="MSDPVAEKVIATLASVKRVPAENIKLDTNLQEMGIDSLDVFTLL
                     FELENAFKISIPDDDVRSIKTVKDVVDGVKKLLGAAPPDTSLGSTSPAS"
     gene            1717..2946
                     /locus_tag="VN788_01570"
     CDS             1717..2946
                     /locus_tag="VN788_01570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008434325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXU19251.1"
                     /translation="MASRRVVITGLGIISPLGLDLEETWDGLRAGRSAIGPIQSVDGS
                     NVGLKIHNGAQVHEFDPLEHFEGGKDAQLDRFAQFSVVAAREAFRDSGIELTPELREQ
                     SAIVCGSAVGGQAAIEAGFEDLWVQGRGRVHPLTIPKTMANAGASHISMDLGLSGPVY
                     TISTACSSANHAIGQAFRLVRDGQAEFAIAGGAEAIFTVGMLKAWEAMRVISPDTCRP
                     FSKERRGMILGEGGAMMALEPFDAARARGAKIYAEICGLGMTSDAHHLTQPTVDGPAR
                     AMRAAMREAGIAPEAVGYINAHGTGTLGNDPVESRAIREVFGAHADKLGVSSTKSMHG
                     HALGAAGALEAVATVLALHHGVLPPTANYLGRDPECDLDYIPNQARAAQVETALSNSF
                     AFGGLNAVIAFRAVPSG"
     gene            complement(3290..4027)
                     /locus_tag="VN788_01575"
     CDS             complement(3290..4027)
                     /locus_tag="VN788_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19252.1"
                     /translation="MSRERVALAGALAGAILLFAGGARGGTLTAASIPASPANPQPGA
                     AFLQDKGTFRILVNGQQVGKEEFEIASRGGDWVAHDTSEIQGAGGMSRVTGTLELHAD
                     GTPAHYEWSTQGEKKATATIGFEGSTATIDLHIEGVKPYTQQFTFNSPQVVILDNNLY
                     YQYAVLASLYDWNRKGTQTFAVLVPQELTPGTVTVESAGTHDAGGKKLEELAVKSPDL
                     EVDLFLDGQRLVRIVSPSANAEILRDK"
BASE COUNT          864 a         1215 c         1197 g          870 t
ORIGIN      
        1 ttgcccagat aaaatttccc acatgcgttc atagacggcc gatttaagtt cgtccggcag
       61 ggcgtcgaag gcgtctgaat agatcatgta gctgcacgga taacgcatca ggcgatgttg
      121 aagatcgaat tgccgcagcg agcggccctg gctgtcggta ggtccctcgg tcgagaactg
      181 ctccgcgaaa cctgatgtgc ctcgaacccg ggaagtcatg ggcgcctcat ccacaaacag
      241 catgtagtcg actaattccc gggcggactc gcgaataatt ctggcggtgg ccgcgtgccc
      301 cctatccggg ttcatttcat cccggtacaa ggaaccgcga acatcccatc ccacacgcgt
      361 gaacaaattc atcatgtgca tctggtgctc gaaaaccatc agtgccacga cgtcgctgta
      421 tggagaaagg taggcagccg tatcgaagtg accttgcaaa gattcaaggt ttcgcggctg
      481 tccttggagc atcgggtgca gccttcccgc tttattgacg acggaattgc ccatgtgcgg
      541 catcgagccg gtcgagccag tcacgtacca gccgccccag cgctgcgcaa aggggctgcg
      601 atcgtcggtg agatattgtc ccaacttcga cacggtcgcg ccatttggcg ccggaaacac
      661 gctacgcatc aaagtgccgg gcactccgag gcttgcgtag gacacgtggc acgccaaaca
      721 gttgacatcg cgcgagaggc ggactttttg cgcggggttc tggctcaact tgtagaaaat
      781 catgccttgc ttaggatctt ccgcagctac ctcgatgatt ggaccgccgc gtacccatcc
      841 cacgaccacc gaatcgttaa agaagagact gcgcggattg tgcggattga tgcgatccgc
      901 ctggaagctc gtcttcgaga aaacgaccaa ttgcgactgg atcggaacgt cgagagcgtc
      961 aagcatcgat cgcaaatagc cctgtgggcc atcaaatttg aggtgaacct cgccgtattg
     1021 gattttgcgg ttgagctgcg ttcctggatc atgaagcgtt tgggtgttgt attgaatggc
     1081 gggctgatcg aggtcggcat atgacataac tgtgccatcg ccaaacgtaa ctgcgatcac
     1141 caagacaatc agcccaggaa ccaagacgcc acgaagcatt cgcacaccag cgaggcctgg
     1201 ataagccccg accgaattgt atcattcgat aagaatgatg cgattgcacg acccaatgaa
     1261 caggggctga ttcccgtgca taggcctttc ggaggccacg tgagaatttg atttctggct
     1321 tgccatacga tacggtctga ttgtacaaac tccacttcta aatcggcgtg cggcccgcgt
     1381 aatgattttc ttaaggacgg tttgatttca ccggcggagg tttaacgtag gatgtctgat
     1441 cctgtagcgg aaaaggtgat cgcgacgctg gcatcagtga agcgtgtgcc agccgagaac
     1501 atcaagctcg ataccaacct gcaggaaatg ggcatcgatt cgctcgacgt gttcacccta
     1561 cttttcgagc tcgaaaacgc cttcaaaatc tcgattccgg atgatgacgt acgctccatc
     1621 aaaaccgtca aagatgtcgt ggacggtgtc aagaaactcc tcggtgccgc cccgcccgat
     1681 acttcgctgg gatcaaccag cccagcgagc tagcgcatgg catcacgccg cgtcgtcatc
     1741 acaggccttg gaatcatttc gccgctgggt ctcgatctcg aagagacgtg ggatggcttg
     1801 cgcgcgggcc gctccgccat cggaccaatc caatccgtcg atggctcgaa cgtgggactc
     1861 aagattcaca acggcgccca agtgcatgag ttcgatccac tagagcattt tgaaggtggc
     1921 aaagatgcgc agctcgaccg cttcgcgcaa ttctccgtgg tcgcggctcg cgaagccttc
     1981 cgcgattctg gcatcgaatt gacgccggag ttgcgcgagc aatccgcgat cgtctgcggc
     2041 tccgccgtcg gtggccaagc tgcgattgaa gcgggctttg aggacctctg ggtccagggc
     2101 cggggccgcg tccacccgct caccattcct aagacgatgg ccaatgcggg cgcgagccat
     2161 atttcgatgg acctcggctt gagcggcccg gtctacacga tctcgacagc ttgctcatct
     2221 gctaaccatg ctatcggtca agcgttccgc ctggtgcgcg atggtcaagc ggaattcgcc
     2281 attgccggag gcgccgaagc aatctttact gtcggcatgc tcaaggcctg ggaagcgatg
     2341 cgcgttattt cgccggacac ttgtcgcccc ttcagcaagg agcgccgcgg aatgattctg
     2401 ggtgaaggcg gagcgatgat ggcccttgaa cccttcgatg cggcgagggc gcgcggcgct
     2461 aagatctatg ccgagatttg cggcctgggc atgacctctg acgcccacca tctgacgcag
     2521 cccacggtcg acggacccgc gcgcgctatg cgagccgcta tgcgcgaagc aggcatcgct
     2581 ccagaagcgg tgggatacat caacgcgcat ggcacaggca cactgggaaa cgatccagtg
     2641 gaatcgcggg ccatccgcga ggttttcggc gcgcatgcgg acaaacttgg cgtcagctcc
     2701 accaagtcaa tgcatggcca cgctctgggc gccgcaggcg cgctagaggc cgttgcgacc
     2761 gtcctcgctc tgcatcacgg agttctgccg cccactgcaa attatctcgg ccgcgatccc
     2821 gaatgcgatc tcgactacat tcccaaccag gcgcgagctg cgcaagtgga aaccgctctc
     2881 tcgaattcct ttgccttcgg cggattgaat gccgtgatcg ccttccgcgc cgtgccctcc
     2941 ggttgaatcg cggtgtatgt tccttccctt ctggaaagcg ctgaagggtt tcccagcgag
     3001 ccgcgcgtca aagtcttcga actcaacctg gaccgcgacg cctttcatct atgcgccaat
     3061 cgcgtgcagc aaatgtctct ggaggctgtt ccacttttgc gacgttgtga ggcggtgagg
     3121 gcgtagtccg aggatacaag cagtattctt acacgagaca atcatacgac gcaagtgctt
     3181 tgttatgtgg agaaaatcgt ccacggtgac ttgtggaagc ggcatgattt cgtggccgcc
     3241 ggaccctctc ccagacgccc ggacgagggg cttgatgcgg acgtcccagt tacttgtcgc
     3301 ggaggatttc agcgttggcg ctgggggaaa cgatgcggac gaggcgctgc ccatcgagaa
     3361 acagatcgac ttccaggtcc ggactcttga cggcgagttc ttcgagcttc ttcccgccgg
     3421 cgtcgtgcgt cccggccgat tcaacagtaa cggtgccggg cgtcaattcc tgcggcacga
     3481 gcaccgcgaa cgtctgcgtc cccttccggt tccaatcgta aaggctcgcg agcaccgcat
     3541 actggtagta caagttgttg tcgaggatga ccacctgcgg cgaattgaac gtgaattgct
     3601 gcgtataggg tttcacgcct tcgatgtgaa gatcgattgt cgcggtggaa ccttcgaagc
     3661 cgatcgtcgc tgtagctttc ttttcacctt gagtggacca ttcgtaatgc gcgggcgtgc
     3721 catccgcgtg gagttctaga gtgccggtga cacgtgacat tcctccggcg ccctgtattt
     3781 ccgatgtgtc gtgagctacc caatcgccgc cgcgcgaagc gatttcgaat tcttccttgc
     3841 ctacctgctg cccattcaca agaatccgaa acgttccctt atcctgtaaa aaagccgcac
     3901 cgggctgtgg attcgccggc gaagcgggga tgcttgcggc cgtcagggtc cctccgcgcg
     3961 cgccaccagc aaaaagcaga atcgcgccgg caagagcgcc agcaagtgcc acgcgctctc
     4021 ggctcatacg gtcacctcgg gaaaaattcc gagcgccagt atctgctcca caacgcgcgg
     4081 cggttccgcg tcgctatcca ccctgtagtc cgagagttcg taagtcggca gacgctcgct
     4141 gtaaag
//
LOCUS       DATNDT010000050         4756 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1656496, whole genome shotgun sequence.
ACCESSION   DATNDT010000050 DATNDT010000000
VERSION     DATNDT010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4756
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1656496"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..622
                     /locus_tag="VN788_01580"
     CDS             <1..622
                     /locus_tag="VN788_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HXU19253.1"
                     /translation="AGLGRNTRYVGWGCGFFDFDNDGWPDLLLVNGHAFPEVDRLHID
                     IHYKDRAILYHNLGNGKFQDISESAGPGILEKHSSRGAAFGDYDNDGLLEVIINNQNE
                     PPSLLKESRPNNNHWTLLKLTGVHANRSAIGAKISLTADGRTQIGEVRSGGSYLSQND
                     MRLHFGLGLAQKIDELRIRWPGGATQIEHDLPVDRILNIMEKARTK"
     gene            complement(675..2504)
                     /locus_tag="VN788_01585"
     CDS             complement(675..2504)
                     /locus_tag="VN788_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXU19254.1"
                     /translation="MKIKENHSRKWITFLAAGILALPVVYAQDLPDGFEISPFGGVSI
                     FRSVKDGLGDNHVTGGAFGARVTENIWNHFGLEESYTYSVNNVEFLTPAVAGAPNYGF
                     GSRLHTFMLNPLLYFTPKGSRVRPYATVGGGAIQYTPTGDAKALARSSNLSAQFGASN
                     LNDNLQAAMSYGGGVKVHLSEHFGVRLDVRGLLSRNPTYSLPNYPTGGVYIQNNSLLH
                     GFQATAGLTFYFGHPKAAAAPPAPAAPAPPPAPPAGPISSGTITGTEGKLCQGRAITL
                     HSTASDPVGHQLKYAWKVNGQEQSSETADLQLTPDHPGSYTIEVTASDPATNRTGTPA
                     TTTINVAEYKEPTATACTATPAELNIGDKAQLSMTGAGSDCSTINYKWTVTEGTVTGE
                     TSANAEFDSSSVQFQPGAQAQTKTVTATGTVTDDQGKSASCTATLTVKSSPKAQRFAD
                     IVYAKDSARVNNCGKRVLLEELAPKAADPDYDVVLVGHIDQDEATPARGRRRAHELDR
                     ERVMNAAAVLSAGKGICAKIDPSRIKIDWVGTDQTADFQPGLCGTSARPASKERKGQG
                     TTEADEKRRVEVWLVPKGAPMPSSVKAAKSLPEHEVRALGCPR"
     gene            complement(2555..4546)
                     /locus_tag="VN788_01590"
     CDS             complement(2555..4546)
                     /locus_tag="VN788_01590"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HXU19255.1"
                     /translation="MVAAARIFLVCAISILFFMPLRAAHWEMAGPYGGSATYVTIDPK
                     QPGHLLLGARDALLYETDDGGASWTRLPLPRQSMGNVRAIVIDPEDAKHYLVGVEAEG
                     SAWAGLYESVDAGEHWSMAPDLKGNSIDALTVFPSDRKRIAAGTRKGVYLSDDNGKTW
                     RAITPDDVLEMRGVTAVAFDPKNSSIIYAGTAHLPWRTSDGGKTWNSIHTGMIDDSDV
                     FSIYVDQAQPDRIFASACSGIYHSADRGESWMKFKGIPHSQRRTHVIRQDPSNPNVLY
                     AGTTEGLLKTADGGETWKQVDTLEINSLAIDPSNPKVLYIAAEYSGLWKSDDGGTTIH
                     SLNRGFAGRRLGKITADGDTVYAAGTLDGGFQGVFRSENGGKTWQETSAGSAWAGHRL
                     VALEGIPGKNLVFASTGDLLYRSSDRGRTWQIVPIRITTVAHVREPRAKARGARSRRV
                     VISSRTVAPKIFALAVMHGAKPELVAATSMGLLRSANLGASFETVRLPGRETTVSLVQ
                     AAPDGSPRLVIRTEEGLLLSEGGSGDWKPLHAPVSASQIYDIAVSPQPDGPVLIGTTD
                     GLYVWRSSDGWAKAKGLPAWTVNAVQFHPVRKGVAYAVQFDHLYESSDGGISWEPYSG
                     ADLGEIYARRLWVSPSRPDRLFAITPDTGVLLLDLSLVQ"
BASE COUNT          945 a         1459 c         1323 g         1029 t
ORIGIN      
        1 ggcgggactc ggccgcaaca cgcgctacgt tggctggggc tgcggcttct tcgatttcga
       61 taatgacgga tggcccgatc tgctgctggt gaacggccac gccttccctg aagtggaccg
      121 tctccacatc gacatccact ataaggaccg cgcaattctg tatcacaatc tcggcaatgg
      181 gaaattccaa gacatctccg aatccgcggg acctggaatt ctcgaaaagc actcatcgcg
      241 cggggcggct ttcggcgact atgacaatga cgggctattg gaagtaatca tcaacaatca
      301 gaacgagccg ccgtcgctgc tcaaggaatc ccgcccgaac aacaatcatt ggacgctgct
      361 gaagctaacc ggcgtacacg ccaaccgcag cgctatcggc gcgaaaatct cgctaaccgc
      421 cgatggccgc acccagattg gcgaagttcg cagcggcgga agctatcttt cacagaatga
      481 catgcgcctg cacttcggcc tcggcttagc ccagaaaatc gacgagcttc gtattagatg
      541 gcccggtggc gcaacgcaga tcgagcatga cctgcccgtc gatcgcattc tgaacatcat
      601 ggaaaaggca cgcaccaaat aaaaaggggc gctgcttttg caacgcccct tcacggttaa
      661 gcgatgtgcc cggtttatct cgggcagccg agtgcgcgta cttcgtgctc cggcagcgac
      721 ttcgcggcct tcaccgaaga cggcatcgga gcgcccttcg gaaccagcca gacttcaacg
      781 cgccgtttct catcggcttc cgttgtcccc tggcctttcc gttccttgga cgccggacgc
      841 gccgacgttc cgcagagacc cggctggaag tccgccgtct ggtccgtacc gacccaatcg
      901 atcttgatgc gcgatggatc gatcttggcg cagatgccct tacccgcact cagcaccgcg
      961 gccgcgttca tcacgcgctc gcgatcgagt tcatgggcgc ggcgtctgcc tctcgccggc
     1021 gttgcttcat cctgatcgat atggccaacc agcaccacat cgtaatccgg atcggctgcc
     1081 ttcggagcca gctcttcaag taggacgcgc ttgccgcaat tgttcacgcg ggcgctgtcc
     1141 ttggcgtaga caatgtcggc gaaccgctgg gccttcggcg atgacttcac cgtcagcgtc
     1201 gccgtgcagg atgccgattt accctggtca tctgtcacgg tgccggtcgc cgtcacagtc
     1261 ttggtctgcg cctgcgcgcc cggttggaac tgcaccgagc tcgaatcgaa ctcagcattt
     1321 gcgctggtct cgcccgtaac ggttccttcc gtcactgtcc atttgtagtt gatcgtgctg
     1381 caatcggaac ccgcgccggt catgctgagt tgggctttgt cgccaatgtt cagttcggcc
     1441 ggagtagccg tgcaagccgt agccgtgggt tctttgtact cggcaacgtt aatagtggtg
     1501 gttgctggag tcccggtacg attcgtcgcc ggatcgctcg cggtcacttc gatcgtgtag
     1561 ctgcccggat gatccggcgt gagctgcaga tcggcagtct cggagctctg ttcctggcca
     1621 ttcaccttcc acgcgtactt caattggtgg ccaaccggat cgctcgccgt tgaatgcagc
     1681 gtgatcgcgc ggccttggca aagcttgcct tccgtgccgg taatcgttcc gctgctaatc
     1741 ggaccggcgg gaggagccgg gggcggcgcc ggagctgcgg gggccggagg agcagccgct
     1801 gccttcggat gtccaaagta gaacgttagc cccgctgtcg cctgaaagcc gtgcagcaga
     1861 ctgttgttct ggatataaac gccgcccgtc ggataattcg gcaacgagta agtcgggttg
     1921 cgcgacagca aaccccgaac atccaggcgt acgccgaaat gttcgctaag gtggaccttt
     1981 acgccgccgc cgtagctcat cgccgcctgt aggttatcgt tcaggttact agcgccaaac
     2041 tgtgccgata agttggagct tcgcgccaag gccttcgcgt ctccggtcgg cgtatattga
     2101 atcgcgccgc cgccaaccgt ggcatatgga cgaacccggg aacccttggg cgtgaaataa
     2161 agcagcggat tcagcatgaa cgtatgcaac cggctgccaa atccgtaatt tggcgcgccc
     2221 gctacagccg gagttaaaaa ctccacgttg tttacgctat atgtatagct ctcttcaagt
     2281 ccgaaatgat tccagatatt ttccgtcact cgagcgccga aagctccgcc cgtgacgtga
     2341 ttatctccta gcccatcttt taccgaccta aaaatactca cccctccgaa cggactaatt
     2401 tcaaatccgt cgggaagatc ctgagcgtaa accaccggta acgccaatat accggcggct
     2461 aaaaacgtga tccattttct cgaatggttt tctttgattt tcatcaactt gcccctccac
     2521 gtagtactca tacatttata actcaatttt gatgtcattg aaccagactc agatctaata
     2581 gcaatacccc cgtgtctgga gtaatcgcga acaatcggtc cggccgcgat ggcgataccc
     2641 acagccggcg ggcgtagatt tcgcccaaat ccgctcccga atacggctcc caggaaatcc
     2701 cgccgtccga tgattcatac aaatggtcga attgcacggc gtaggccact cccttccgga
     2761 cgggatgaaa ttgcacagcg ttgaccgtcc aagcgggcaa tcctttcgct ttggcccagc
     2821 catcgctgct tctccatacg tacaggccat cggttgtccc gatcagtacg ggaccgtccg
     2881 gttgcggaga aacggcgatg tcgtaaatct gcgacgcgct cactggcgca tgaagtggtt
     2941 tccaatcgcc ggaaccgcct tcggaaagta agagcccttc ttcagtccga atcacgagcc
     3001 gcggcgaacc gtccggcgcg gcctgaacca atgagacggt agtttcgcgg cccggcagac
     3061 ggacggtctc gaagctggct cccagattcg cgctacgcag caatcccatc gatgtcgcgg
     3121 caaccaactc tggcttagcg ccgtgcatga ccgccagcgc gaaaattttc ggggcgacgg
     3181 tccggctgga tatgactaca cgccgggatc tggctccccg cgccttggct cgcggctcgc
     3241 ggacgtgagc caccgttgtg attcggattg gaacgatctg ccacgtgcgg ccccggtcgg
     3301 atgatcgata aagcaggtcg ccggtcgatg cgaaaacgag attctttccg ggaatccctt
     3361 cgagcgcgac cagcctgtgg ccagcccacg cgcttccggc ggatgtctcc tgccaggtct
     3421 tgccgccgtt ttcactgcga aatacgcctt gaaaaccgcc atccagcgtc ccggccgcat
     3481 acacggtgtc gccgtccgcc gtgatcttgc cgaggcgcct tcccgcgaat ccgcggttca
     3541 acgaatgtat cgtcgttccg ccgtcgtcgc ttttccaaag acccgaatac tccgccgcga
     3601 tatagagaac tttgggattc gaaggatcga tcgccagaga attgatttcg agtgtgtcca
     3661 cctgcttcca ggtctcgccg ccatccgcgg tcttcagcag cccttcagtc gtcccggcgt
     3721 aaagaacatt cggattcgat ggatcctgcc ggatcacgtg cgtgcggcgc tggctatgag
     3781 gaatgccttt aaacttcatc caggattccc cgcgatccgc gctgtggtaa atgccgctgc
     3841 acgcgctggc gaaaatccgg tctggctggg cctgatcgac gtagatggag aaaacatccg
     3901 aatcgtcgat cattcctgtg tgaatagagt tccaagtctt gccgccatcg ctggtccgcc
     3961 aaggtaagtg ggcggttccg gcgtagatga tggatgagtt tttcggatcg aatgccaccg
     4021 ccgtaacgcc gcgcatttcc aggacgtcgt ccggagtgat ggcgcgccac gttttgccgt
     4081 tgtcgtcgct cagatacact cccttgcgcg tgccggcggc gatccgcttg cgatcgctcg
     4141 ggaataccgt gagagcgtcg atggagttgc ctttgagatc cggcgccatc gaccagtgct
     4201 cgcccgcgtc cacactctca tagagtccgg cccatgcgga gccctccgct tcaacgccaa
     4261 ccaagtaatg ttttgcatcc tccgggtcga tcacaatcgc ccgcacgttg cccatcgatt
     4321 ggcgcggcag gggaagccgt gtccatgatg cgccgccatc gtccgtttca tacaacagag
     4381 cgtcgcgggc gcccaggaga agatggcccg gttgcttggg atcgattgtc acgtacgtgg
     4441 cgctccctcc atagggaccg gccatttccc aatgagccgc gcggagcggc atgaaaaaaa
     4501 gtatggaaat cgcgcaaacg agaaaaatac gagcggcggc taccattggt gcgccgccca
     4561 cctcatgcta aatagagcct tgccacgtac aaacgtttcg ttcattttcc ggacttaatc
     4621 cgaagcaatc tcgatgaaca cagttcttgg acgcgttcct ccagaagagt agtgacgatt
     4681 cgaggccgtg cgcatcggtt ttctttattc cgttatagct tgttcggaga gtcgggttgt
     4741 caacgcggtc tctagt
//
LOCUS       DATNDT010000051         7208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1356018, whole genome shotgun sequence.
ACCESSION   DATNDT010000051 DATNDT010000000
VERSION     DATNDT010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7208
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356018"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(231..1727)
                     /locus_tag="VN788_01595"
     CDS             complement(231..1727)
                     /locus_tag="VN788_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19256.1"
                     /translation="MLSALFVAVAILFVIAAVRAEKISFIRNSAFFLNPDGASQTYSA
                     NGGGIDLTGPFFQSLGTNGRSCGTCHQPSDGMSVSAAHVQERFEQTRGEDPIFRTVDG
                     SNCDHGIDVSRLDRKFAAYSLLRTRGLFRIGIAVPANADYRVVSVQNPYGCNESDTIS
                     MYRRPLPATNLRFLSAVMTDGRESTPLTGTQKIEFANYPGSLLSDLAHQAMDATMGHA
                     QGDGSRPTPAELQGIVDFEMSVSTAQAIGRGTGRLDAEGANGGPVALATQPFFVSINS
                     SIDPLVPQFEPPGGLSVPGEGQSSATIFNLFDAWASHGDASPRAAIARGQAIFNSKPI
                     NITGVAGINDDISAGGLVRGGIPSLTGTCGTCHDTPNVGNHSFATALNIGTGDPDPYD
                     SSVNTGGLDIRYLPSITVCRADPISGGPTSECKTTSDLGQALIDGRFDHVGKVKGPIL
                     RGLSARAPYFHNGSAENLLDVVHFYEGRFGLVLTPQEESDLVAFLSSL"
     gene            complement(1996..4428)
                     /locus_tag="VN788_01600"
     CDS             complement(1996..4428)
                     /locus_tag="VN788_01600"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXU19257.1"
                     /translation="MSTLSPDHWQEISPYLDEVLSLPEQEREKWFDAFRRRRPDLLDL
                     IQKLLNEHSTLAKEGFLERGPVTPASEAALAGRIVGAYKLLSPIGQGGMGSVWLAERS
                     DGRFERQVAVKFLNFAVAAQGAERFKREGSILGRLAHPHIAELMDAGVTENGEPYLVL
                     EHVEGDHIDEYCDQRSLDVNARVRLFLDVLSAVAQAHANLVVHRDIKPSNVLVGNDGC
                     VKLLDFGIAKLLTDDASPTTPTLLTVEGGGALTPQFAAPEQITGGAVTTATDVYALGV
                     LLYLLLTGRHPAGAPAQSTAALVKAIVDTEPQRASEAVDSDDATARATKRASTPDKLR
                     RQLRGDLDTILAKALKKNPAERYSSVIAVADDLQRYLRQEPIAARPDTFGYRAAKFVR
                     RNRSAVALSALAFLTIISGVTGTLLQARAARRQRDAAVRERDRATRITDFMTNMFKQS
                     DPIEARGNTITVREMLDKASKDINTGLGEDPETQAQMMDVMGSVYDNLGLYPSAESLL
                     RRAVKIRGAVLGPEHVDTLGSKRSLAFVLQEEGHFAEAEKLQRETVEDARRGLGPENP
                     FTVRCLGDLGGDLELQGRYTEAEKLLREAIDKDRRVLGPENEVTLAATSNLASSLYMQ
                     GRYADAEKVQRETLDIDRRIFGTSDLTTLGLMDNLALTLREEERYPEAETLERETLDT
                     DRRVLGPEHPNTILATETLGLILKREGHYAEAEKLIRQTIDLQRRVLGPDHWQTAESI
                     YNLGCLAAVQGKRDKAISLVRDAVDHGFIPQYDVSLIEQDSDLKLLHGDSRFAALLAH
                     ARERAAAAKKPD"
     gene            complement(4435..5004)
                     /locus_tag="VN788_01605"
     CDS             complement(4435..5004)
                     /locus_tag="VN788_01605"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HXU19258.1"
                     /translation="MENPPTLSSLMDAAERGNRPAADALFAALYAELRRLAKHELARQ
                     GAPLSLSATTLLHKAYIEIARQDGTSFADRSRFMGYAARVMRGLIIDFARNRHAQKRG
                     GEFHITSLGPEAENAVDCGKLIQVSEALDELAQAEPELADVVDMRFFCGFTFAEIAAM
                     KGVSERTLQRQWEKARIYLHHRIRGDLEV"
     gene            complement(5285..6685)
                     /locus_tag="VN788_01610"
     CDS             complement(5285..6685)
                     /locus_tag="VN788_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007269569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyltransferase family protein"
                     /protein_id="HXU19259.1"
                     /translation="MGQQSFEDVLKAAGNPAQMLRNSQIGAYVYPVVPSEYSNWRDEQ
                     KAWRETAVLFDQSHHMANLYVEGPDALKMLSHLATNSFANFPVNRAKQFAPCTHYGYV
                     IGDGILFHLEQDKLVYVGRAPAAEWLEFHGSTGNYNVKITRDDRSPSNPGGRPVIRTL
                     YRYQIQGPNAKQIIEKLNGGPFPEIKFFNMGEIKIKGRTVRALRHGMAGAPGLEIWGP
                     YEQRDEIRAAIMEAGREFGIKEVGARAYASNTCESGWIPSPLPAVYTGEKLKPYREWL
                     PANSYEATGSLAGSFVSNNIEDYYTTPYELGYGIFVKFDHDFIGREALEKIDQKTQRK
                     KVTFAWNSDDVVKVYKSLFEQGDPYKFIDLPISNYASSSYDKLVGKGGKIVGLSMFAG
                     YSYNERSMLSLGVVDHDVEIGTELTLVWGEEGGGSKKPTVERHKQMEIRAVVSPVPYS
                     QVVRETYAEGWRKHQK"
     gene            complement(6887..>7208)
                     /locus_tag="VN788_01615"
     CDS             complement(6887..>7208)
                     /locus_tag="VN788_01615"
                     /inference="COORDINATES: protein motif:HMM:NF039925.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5938 domain-containing protein"
                     /protein_id="HXU19260.1"
                     /translation="PKAEQEKKLAEMANAVQAGTPPRENSREQRTIDVVVGRGSLETA
                     QVVLFGTCCYKQTGLLQAWGAHSLVHCPPRRVGYASPAAAFGHREILKTLENHGLSRA
                     KTYT"
BASE COUNT         1393 a         2210 c         2025 g         1580 t
ORIGIN      
        1 gctctacgtt gacgctttcg gcttcgcccg cgggggaatc accggcagcc tcgccatcga
       61 tgccatcaca ggcatcttcg ccagcgtcct gcttggcctc atgctgctct cgctctcccg
      121 caggcccaaa ccactgccag cctaaccgcg acggcccaag cgcctcggca cgccatcgcc
      181 aataacttcc ggccttctca tggaaggact tacatcaatc gccgacgcag ctagagtgag
      241 ctcaagaacg ccaccagatc cgattcctcc tgcggcgtta ggacaagccc gaagcggcct
      301 tcataaaaat gcactacatc caataggttc tcagcggagc cgttgtggaa gtacggagcg
      361 cgggcggaga gtccgcgcag gatgggaccc ttgaccttgc ccacatgatc gaaccggcca
      421 tcgatcagcg cttggccgag atctgaagtt gtcttgcact cgctcgtggg cccgccgctg
      481 attggatccg ctcgacacac cgtgatggaa ggcaggtacc gtatatccag gcctcccgtg
      541 ttcacggacg aatcgtaagg atccgggtcg ccagtcccga tattcagcgc cgtagcgaat
      601 gagtgattgc ccacgtttgg cgtgtcatga catgtgccgc aagttcccgt tagcgaaggt
      661 atgccgcccc gaaccaatcc tccagctgaa atgtcgtcgt tgatgccggc cacgccggtg
      721 atgttgatcg gcttggaatt gaaaatggcc tgcccgcgcg cgatggcggc ccgcggcgac
      781 gcgtcgccgt gtgacgccca tgcatcaaag agattgaaaa tcgtggcact ggactgcccc
      841 tcgcccggca cgctcagccc gcccggcggc tcaaattgcg gcacgagagg atcgatgctg
      901 gaattgatgc tgacgaagaa cggctgagtg gccagagcaa caggcccgcc gttcgcgccc
      961 tcggcatcta ggcgcccagt tcctctcccg atcgcctgcg ccgtagagac cgacatctcg
     1021 aaatctacga ttccctgcag ttctgcggga gttggtcgcg acccgtcccc ttgtgcgtgg
     1081 cccatcgtag catccatggc ctggtgcgcc aggtcgctca gcagggaacc gggataattc
     1141 gcgaactcga tcttctgtgt gccggtgagc ggcgtcgatt cgcgcccgtc tgtcatcact
     1201 gcgctcaaga agcgaaggtt ggtggcgggc agcggccgtc ggtacatcga aatcgtgtcg
     1261 ctctcgttgc agccgtatgg attttgaacg ctaacgactc ggtagtcggc gttcgcggga
     1321 acagcaattc caatacgaaa caggccgcgc gtccgcagaa ggctataggc ggcgaatttc
     1381 ctgtctaacc tggagacgtc aatgccatga tcacagttgg aaccatccac ggtgcggaaa
     1441 attggatcct cgccccgggt ctgctcgaat cgctcctgga cgtgggcggc ggaaacggac
     1501 atgccgtcgc tgggctggtg gcaggtaccg catgaccggc cgttagtgcc caggctttgg
     1561 aaaaatggtc ccgtgaggtc tatgcctccg ccgttggcgc tgtaggtttg tgacgcgcca
     1621 tccgggttca gaaaaaacgc gctattccga atgaaggata tcttctccgc cctcaccgcg
     1681 gcgatgacga ataggatggc gacggccaca aaaagggcgc ttaacatgcc acggccgcgt
     1741 gagcgcactg atttggatcc tttagcaaag aggtgagaca caaaacggcg agggcggcac
     1801 tgccttgctg tgggaatctg cattggaaca cctcttacgg ccccggcggc acaccttcgc
     1861 tcacaaagtt gctggcgacc gtgaatcggt ccgctggagc gttgagccga atgatacgct
     1921 ctccggcttc tacttaggta atcgtaaacg gccggtaaaa gacgacaaaa ccggcgcgga
     1981 aaacggacgg gaattttagt ccggcttctt agctgcggcc gcgcgctccc tggcatgggc
     2041 cagcaaggcc gcgaagcggg aatccccgtg aagcagtttc aaatcgctgt cctgttcgat
     2101 cagactaacg tcgtactgcg gaatgaaacc gtggtcgacg gcatctcgaa ccagcgaaat
     2161 ggctttgtcc cgcttgcctt gcaccgccgc taaacagccg aggttgtaaa tcgattccgc
     2221 tgtctgccag tggtctggac ccagcacgcg gcgctgaagg tctatcgttt ggcggatcaa
     2281 cttctccgcc tcggcataat gaccttcccg cttaagaatc aaaccaagag tctccgtcgc
     2341 aagtatcgtg tttggatgct caggcccaag gacgcggcga tcagtgtcga gcgtctcgcg
     2401 ctctagcgtt tccgcttcgg ggtaacgctc ttcctcccga agagtcaaag caaggttgtc
     2461 cattaatccc aaagtggtga ggtcactcgt gccgaagata cgccggtcaa tgtcgagcgt
     2521 ctcgcgctgc actttctccg catctgcata gcggccttgc atatagaggc tggatgcaag
     2581 attggatgtc gccgcaagcg tgacctcatt ctccggcccg aggacacggc ggtccttgtc
     2641 aattgcctcg cgcagcaact tctccgcttc cgtataacgg ccttgaagtt cgaggtcccc
     2701 tcccagatcg cccaggcatc tcaccgtgaa cggattctct ggcccgagtc cgcggcgcgc
     2761 atcctcgact gtttcgcgct gcaacttttc cgcctcggcg aagtggccct cctcctgaag
     2821 aacgaatgcc aatgagcgct tcgatcccag cgtgtccacg tgttccggtc cgaggacggc
     2881 gccccgaatc tttacggcgc gcctcaataa agactccgca cttggataga ggccgagatt
     2941 gtcgtaaacg cttcccatga cgtccatcat ctgtgcctga gtctcaggat cctcacctag
     3001 acctgtattg atgtccttcg aagctttgtc gagcatttcg cggacagtga tggtgttccc
     3061 gcgtgcttcg atcggatctg attgcttgaa catattggtc atgaaatcgg tgatgcgagt
     3121 ggcgcggtcg cgctcgcgaa ctgctgcgtc gcgttggcgg cgagccgcgc gggcctgaag
     3181 cagcgtgccg gttacgccgg aaatgatggt gaggaaggca agcgcggaca atgcgacggc
     3241 gctgcggttc cggcggacga acttcgcggc ccggtaaccg aatgtgtcgg gccgggctgc
     3301 gataggctcc tgtcgcaagt atcgctggag atcgtccgcc acagcgatca ccgagctgta
     3361 gcgttcagcc ggattcttct tcaatgcctt ggcaagaatc gtgtcgagat cgccgcgaag
     3421 ctgccgtcgt agcttgtctg gcgtggaagc tctctttgtg gcgcgtgcgg tggcatcatc
     3481 cgagtcaact gcttcagagg cgcgctgcgg ttccgtatcg acgattgcct tcaccagagc
     3541 ggccgttgat tgcgccggag cccctgcggg atgccgtccc gtcaacagca agtacaacag
     3601 cacgcccagc gcatacacat cggtggccgt cgtgacggcc ccgccggtta tttgctcggg
     3661 tgccgcaaac tgcggggtca gagcgcctcc gccttcgacc gtgagcagcg tcggcgtcgt
     3721 ggggctcgca tcgtccgtga gtagtttcgc gatgccaaaa tccagcagct ttacgcagcc
     3781 gtcgtttccg acgagcacgt tcgaaggctt gatgtcccgg tgaacgacca aattggcatg
     3841 ggcctgcgcc accgcgctga gcacatccag gaaaagtcgg actcgtgcgt tcacatcgag
     3901 cgagcgctgg tcgcagtact cgtcgatgtg gtctccctca acatgttcga gtacaaggta
     3961 cggttctccg ttctcagtga ctcccgcatc catgagttcc gcgatgtgag gatgcgccag
     4021 ccggccgaga atgctgcctt cgcgcttgaa tcgttccgct ccctgcgcgg ccacggcgaa
     4081 attcagaaac ttgaccgcca cttgccgctc gaagcgcccg tcgctccgtt cggcgagcca
     4141 tacgctgccc atcccgcctt gcccgatcgg cgagagtagc ttgtaagcgc ctacgattcg
     4201 gcccgcaaga gctgcctcac tggcaggcgt tacgggaccg cgttcgagga atccctcctt
     4261 tgccagcgtg ctgtgctcgt tcagcagctt ttggatgaga tcgagtagat ccggcctccg
     4321 cctgcgaaat gcatcgaacc acttttcgcg ctcctgctca ggaagcgata gcacctcgtc
     4381 caggtaagga ctgatttcct gccagtgatc ggggctcagc gtggacacgg ccgctcaaac
     4441 ctccaaatct ccgcggattc tgtggtgcag gtaaatgcgc gccttctccc attgccgctg
     4501 tagtgttcgc tccgagacgc ctttcatcgc cgcgatctcc gcgaacgtaa atccgcagaa
     4561 gaatctcatg tccaccacat cggcgagttc cggttcagct tgggccaact cgtccagcgc
     4621 ctcgctcacc tgaatcagct tgccacagtc caccgcgttc tcagcttcgg gccccagtga
     4681 agtgatgtga aattccccgc cgcgtttctg cgcgtggcgg tttcgcgcaa aatcgataat
     4741 caatccgcgc atcacgcgag cggcgtagcc catgaatctg ctgcgatcgg cgaacgacgt
     4801 tccgtcctgc ctcgcgattt cgatgtatgc cttgtgtagc agcgttgtgg cgctgaggct
     4861 cagcggcgcg ccctgccggg ctaattcatg cttcgcgagc cgccgcaatt ccgcgtaaag
     4921 ggcggcgaag agtgcgtccg cagccggcct gttcccgcgt tcggccgcgt ccatgagtga
     4981 ggaaagagtg ggcgggtttt ccaacgagcg tgcctgccta gactccgtat cagaagtctg
     5041 gtctcaaaaa atagctaatc ttgcccgcca ttgtagcctg aatttacaaa cgtagcacct
     5101 acctgtgccg ccaaagaggg ggctgaactt ccgatcagtc gtcaactcgg aagtaggtct
     5161 gattttcgcc gactggttgc ccacgcagct ttttaagcgt ctaaatggtt ttacgaagtc
     5221 aaaaaagaag ggcggatgct caagcacccg cccttcttcg tttccaattg gcttcgttct
     5281 tggcttactt ctgatgtttt ctccaacctt ccgcgtaggt ctcgcgtacg acctgcgaat
     5341 acggcactgg gctgacaacc gcgcgaatct ccatctgttt gtggcgctcc accgtcggct
     5401 tcttcgagcc gccgccttct tcgccccaca ccagcgtgag ctccgtgccg atttcaacgt
     5461 cgtggtccac cacgccgagt gacagcatgg accgttcgtt gtagctgtag cccgcgaaca
     5521 tcgaaaggcc cacgatcttc ccgcccttcc ccacaagttt gtcgtaggaa gacgacgcgt
     5581 aattcgaaat cggcaggtcg ataaatttgt acgggtcgcc ctgttcgaat agcgacttgt
     5641 aaaccttcac cacatcgtcc gaattccaag caaacgtcac ctttttccgc tgggtcttct
     5701 gatctatttt ctccagcgcc tcgcggccga tgaagtcgtg gtcgaacttg acgaaaatcc
     5761 cgtagcccag ttcgtacggg gtcgtgtagt agtcctcgat gttattcgag acaaagctgc
     5821 cggccagtga tcccgtcgct tcgtagctgt ttgcagggag ccactcgcga tatggcttca
     5881 acttttcgcc cgtgtacacc gccggaagcg gcgaaggaat ccagcccgat tcgcatgtgt
     5941 ttgatgcgta tgcgcgcgcg ccaacttcct ttatgccgaa ttctcgtccc gcttccataa
     6001 tcgccgcgcg aatttcgtct cgctgttcgt acggtcccca aatttcgagt cccggcgcgc
     6061 cggccatgcc gtgacgcagc gcgcgcaccg tcctcccctt gatcttgatc tcgcccatgt
     6121 tgaaaaattt gatttccggg aatggcccgc cgtttaattt ctcgatgatc tgtttcgcgt
     6181 tcggcccttg aatctggtag cgatagagcg tgcgaatcac gggcctgccg ccgggattcg
     6241 aaggcgaccg gtcgtctcgc gtgatcttca cgttgtaatt tcccgtcgag ccatggaact
     6301 cgagccattc cgccgcgggc gcgcgcccca cgtaaaccag tttgtcctgt tccaggtgaa
     6361 ataaaattcc gtcgccgatc acgtagccat agtgggtgca cggcgcgaac tgcttcgcgc
     6421 gattcaccgg aaaattcgcg aagctgttcg tcgccaaatg cgaaagcatc ttcagtgcgt
     6481 cgggaccttc cacgtacaaa ttcgccatgt ggtgcgactg atcgaaaaga acggccgtct
     6541 cgcgccacgc tttttgctcg tcgcgccaat tcgaatactc ggaaggaacc accggataga
     6601 cgtacgcgcc aatctgcgaa ttccgcaaca tttgcgcggg attgccggcg gcttttagca
     6661 catcctcaaa actctgctga cccatcgctt ccctcctagg gacagaaacc attggattca
     6721 cctcctattt atcagtgctg ccgactatca ctcaggctca atgttatgcg atcgccgtgg
     6781 ctaaaagtgc acgcaatacc gtacagaaac ttagacgcat ttgcaactag actggaacac
     6841 gaaaagcttc tagttcgaag catttaatcg ttctaactcg aaacaattaa gtgtacgttt
     6901 tggccctgga aagcccgtga ttctccaaag ttttgaggat ttcgcggtgg ccaaacgcgg
     6961 ccgccggcga cgcgtacccc acccgccgcg gcgggcagtg aaccaggctg tgcgctcccc
     7021 acgcttgcag caaacccgtt tgtttgtaac agcaggttcc aaacaggacc acctgcgcgg
     7081 tttccagact cccgcgccca actacgacgt cgatagtacg ttgctcccgg ctgttttcgc
     7141 ggggcggggt tccggcttgg acggcgtttg ccatttcggc tagttttttt tcctgctcgg
     7201 ccttgggc
//
LOCUS       DATNDT010000052         2540 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3645492, whole genome shotgun sequence.
ACCESSION   DATNDT010000052 DATNDT010000000
VERSION     DATNDT010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3645492"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..722
                     /locus_tag="VN788_01620"
     CDS             72..722
                     /locus_tag="VN788_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXU19261.1"
                     /translation="MTLSLGKITAICFAGSILLMGASALTSQTASSSQAPSQPADTSQ
                     SQTTPPSSTDSNGASTSTAAAPAQASTTPLDPWPAGPGKDIFLQTCSSCHGPETVIGH
                     NLDVSGWTDVLNQMIQNGAQGSNDDFSAILQYLVTNFGPMPAKVNINKATAMNLRSWL
                     GMPEKEAEAIAAYRQQHGDFKTLDDLKKVPGVDANYIDSEKNLLTFSDSAAGTAGH"
     gene            complement(719..1774)
                     /locus_tag="VN788_01625"
     CDS             complement(719..1774)
                     /locus_tag="VN788_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19262.1"
                     /translation="MPNTNYRGRRAVSIENENLRVTVVQEGGHVAELLDKRTGVNPLW
                     APPWPSIEPSTYSAAKFPEYGASGEAKLLAGILGHNLCLDIFGGPSVEEAAAGMEPHG
                     EGSIVPYDITESRGMIEMRAIFPLAQIHFERRIELHQANVRFLETVKNLTGVDRPIGW
                     TQHVTLGPPFLERGSTQFRATATRSVVLGGDFSPANYLKGGAEFEWPNAPLKDGGASD
                     LRVFNSAAVSGAYTAHLMDPAREHAFFTAFSPSLKVAIGYIWRTRDFPWMGIWEENCS
                     RMHAPWNGKAITRGMEFGVSPFPESRREMVERGKTFGVPGFRWLPAKGTLKAEYWASV
                     RTEQTTPESMSWPSLSS"
     gene            complement(1874..>2540)
                     /locus_tag="VN788_01630"
     CDS             complement(1874..>2540)
                     /locus_tag="VN788_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU19263.1"
                     /translation="SQFLQDWTISQDLPQGGEGLWRLDVTVAGSGVTGDLLAHNIDMA
                     MYLNGPITEVTAMTETFIKERKHNLTGKVQPVGIDDASAFLCRFQNGSMALFEATRYA
                     RGHKALFTLEINGEKASASWDLHDLHRLEYFDHRDEGRLRGWKSVHVTDGDHPYMKHW
                     WVPGLQIGYEHTFIHQVADFLGAAASGKSAAPTFRDGLATDYVTDAVLKSAKSKQWEK
                     VSG"
BASE COUNT          564 a          743 c          706 g          527 t
ORIGIN      
        1 tgaatgggta aagtatcggc atttattggc gtaagcagct tattttacat actttctcag
       61 gtgatatggc tatgacgctc tctttgggaa aaataaccgc aatttgcttt gccggatcga
      121 tactgctgat gggcgcatcc gccctgacgt cacaaacagc gtcatcctcg caagcgccgt
      181 cacaaccggc cgacacatca cagtcgcaaa caacgccgcc gtcgagcacc gactcaaacg
      241 gagcgagcac atcaactgcc gcagcgccgg cgcaggctag cacgacaccg ctagatccgt
      301 ggcctgcggg ccccggaaaa gacatctttc ttcagacctg ttcatcgtgt catggacctg
      361 agaccgtgat cgggcacaac ctggatgtga gcggctggac cgacgttttg aatcagatga
      421 tccaaaacgg tgctcagggc agcaacgacg acttcagcgc cattcttcaa tacctggtga
      481 cgaacttcgg tcccatgccc gccaaggtga acatcaacaa agcgactgcg atgaatcttc
      541 gcagctggct gggtatgccg gaaaaggaag cggaagcaat cgccgcctat cgccagcagc
      601 acggtgattt caaaacgctt gacgatttaa agaaggtacc cggtgtcgac gcaaattaca
      661 tcgactccga gaagaacctc ctcacgttct ccgacagcgc ggctggcacc gccgggcact
      721 aactcgatag cgaaggccag ctcatcgatt caggcgtggt ctgctctgtt cggaccgatg
      781 cccaatattc ggctttgagc gttccctttg ctggaagcca acgaaatccc ggcacgccga
      841 aggtcttgcc ccgctcgacc atttcacggc gcgattcggg aaacggcgag acgccaaatt
      901 ccattccccg cgtgatggct ttgccgttcc agggcgcgtg catgcggctg caattctctt
      961 cccagattcc catccacgga aagtctcgcg ttcgccagat gtagccaatg gcaactttta
     1021 acgaaggaga aaaggccgta aaaaacgcgt gctcgcgggc cgggtccatc aagtgcgccg
     1081 tgtatgcacc cgaaacggcg gcgctattaa atacgcgcaa atcggacgca ccgccatctt
     1141 tgagcggagc gtttggccat tcgaactcgg cgccgccctt caggtaattc gcgggactga
     1201 agtcgccgcc tagcacaaca gaacgggtag ctgtggcgcg gaattgcgtg ctcccacgct
     1261 caagaaacgg cggaccgagc gtgacgtgtt gcgtccagcc aatcggccgg tcgacgcctg
     1321 taaggttctt aactgtttcc agaaatcgga cgttcgcctg atgcaattcg atgcggcgct
     1381 cgaaatgaat ttgcgcgagt ggaaatattg cgcgcatttc gatcatcccg cggctctcgg
     1441 tgatgtcgta aggcacgatg gacccttctc catgcggctc cattcccgct gccgcttcct
     1501 ccacagaggg accgccaaag atgtcgagac acaaattgtg cccaagaatg cccgcgagta
     1561 gctttgcctc gccgctggcg ccgtattcgg gaaatttggc cgcggaatac gttgaaggct
     1621 cgatgctcgg ccagggaggc gcccaaagcg gattcacgcc cgtgcgctta tcgagaagct
     1681 cagccacatg gccaccctct tgaactacgg tgacgcggag attttcgttc tcgatggaaa
     1741 ctgcgcggcg gccacggtag tttgtattgg gcaagacttg cctcgcggaa cacgcgggcg
     1801 cagcaagcag cgccctacga aatagaacgc acagggacgc ggcgaacagc ggccctgcgg
     1861 atggtttgac agtctatccg gaaacttttt cccactgctt tgacttggct gatttcagta
     1921 cggcatccgt gacgtagtcg gttgccagac catcgcggaa cgtaggtgcc gccgacttac
     1981 cgctcgcagc tgctccgagg aaatcagcga cctgatggat aaaagtgtgc tcgtagccga
     2041 tctgtagacc gggcacccac cagtgcttca tatatggatg gtcgccgtct gtgacgtgaa
     2101 cgctcttcca gccgcgcaga cgtccttcgt cgcggtgatc gaaatattcg aggcgatgca
     2161 ggtcgtgcaa gtcccatgag gccgatgcct tctcaccgtt aatttccagc gtaaacagag
     2221 ctttgtggcc acgggcataa cgcgtcgctt caaaaagagc catcgagcca ttctggaatc
     2281 ggcagaggaa cgcgcttgcg tcatcgatgc ctaccggctg aacttttccg gtgagattat
     2341 gcttgcgctc tttgatgaat gtttcggtca tggcggtgac ttcggtgatc ggaccattga
     2401 ggtacatcgc catgtcgatg ttgtgcgcca gcaggtcgcc tgttacgccg cttccggcaa
     2461 ccgtcacgtc gagacgccac aagccctcac cgccctgcgg caaatcctga gagatcgtcc
     2521 agtcctgtaa aaactgagaa
//
LOCUS       DATNDT010000053         2723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2808339, whole genome shotgun sequence.
ACCESSION   DATNDT010000053 DATNDT010000000
VERSION     DATNDT010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2808339"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1218
                     /locus_tag="VN788_01635"
     CDS             <1..1218
                     /locus_tag="VN788_01635"
                     /inference="COORDINATES: protein motif:HMM:NF012544.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXU19264.1"
                     /translation="WSKEAFGEFHGFVAGWTYWIYTFFYFPGLLVASVAMAAYIGGSG
                     SAHLADSRTFLVVGSFVMLFAAVFLNIIGLNIGKWLQNAGGVGTYIPLLMLIGIGAYV
                     WHTHGSVTHFTASNIRPVWDWGTVNFWPQIAFAFTALELCSTMSEEVREPHKTFPRAI
                     LGSGVLIALAYIAGTFALLAILPAGSVDPKSGVFQAITAASTSSGIAFFGVIAAILVL
                     FGNAGGVGTTVAGVARIPFVVGIDHYMPAAFGKIHPKWKTPWVSILVQAGISCVILLL
                     IQLNETANNAYQIFVDAGTILYFIPFLYMYAAAIKLSYRADRKANPNAALIPGGRLGV
                     WLVGVLGFAVVVGGIALSFIPPAEATNKWLFEAKLIAGTVGGVLLGLILYVRGARAKA
                     REAARAGFTETRH"
     gene            complement(1344..2186)
                     /locus_tag="VN788_01640"
     CDS             complement(1344..2186)
                     /locus_tag="VN788_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19265.1"
                     /translation="MTNSKMKFALSVALAAVSLGALGTVVHAQGQNQANQNKADQNKQ
                     EDVFPEGALAPDNLNKPRPKPPFNLTGTWMVDLSSGFNSFMFGPPYPHFHPAAEALFE
                     EGKKATATGHAFHDDIGKCYPAGMPMIMTRVWPIAMIQTPTVIYMVSGFENEIRLIYI
                     DGRKHTAPDVVVRTYDGDSVGHWEDDTLVVDTTSMTTARHWIDVGVPVSDDFHIIERI
                     RMQNGGKTLEIKYTLLDPKNWDGEWTSTKHWNREDDTDITEVECLPDLDQHLPSTQSK
                     LNIN"
     gene            complement(2262..2651)
                     /locus_tag="VN788_01645"
     CDS             complement(2262..2651)
                     /locus_tag="VN788_01645"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXU19266.1"
                     /translation="MKTSRIAFAVALVFAFAGSAFAHHSAAMFDRDKKVELVGTIVQF
                     SWSNPHSWISIDVPNANGGTDRWGVECNSPNTMARTGWRSTTLKPGDQVTVTIHPLRS
                     GEKGGRFVSVKLADGQVLTDMIPYPTK"
BASE COUNT          491 a          775 c          773 g          684 t
ORIGIN      
        1 tggtccaagg aagctttcgg cgaatttcac ggttttgttg ccggctggac atattggatt
       61 tacaccttct tttatttccc cggacttttg gtggccagcg ttgcgatggc ggcctacatc
      121 ggcggaagcg gttcagcgca tctggcggat agccgcacct ttttagtggt gggctcgttc
      181 gtgatgctgt tcgcggccgt atttctcaac atcatcggcc tgaacatcgg gaaatggctg
      241 cagaatgcgg gcggcgtggg cacgtacatt cctttgctga tgctgatcgg catcggagcc
      301 tacgtctggc acacgcacgg ttccgtcacg catttcactg cgtcgaacat tcgacctgtg
      361 tgggactggg gcaccgtgaa tttctggccg cagattgcgt ttgcgttcac cgccctcgaa
      421 ctttgctcga cgatgagcga ggaagttcgc gaacctcaca aaacttttcc tcgtgcaatc
      481 ctcggctccg gagttttgat cgcgctcgcg tatatcgccg gcactttcgc gttgcttgcg
      541 attcttcccg ctggttcggt ggatcccaag agcggcgtct ttcaggccat cactgcggcc
      601 tctacgtcat cgggcatcgc atttttcggc gtcatcgccg ccatcctcgt gctctttgga
      661 aatgcgggcg gagtgggaac cacggttgca ggcgtcgcca gaattccctt cgtggttggc
      721 atcgaccact acatgcccgc cgccttcggc aaaattcatc ccaaatggaa gacgccctgg
      781 gtttcgattc tggtgcaagc gggaatttcc tgcgtgattt tgctgctgat tcagctcaac
      841 gagaccgcca acaacgccta ccagattttc gtggatgccg gcaccatcct ttatttcatt
      901 cccttccttt atatgtacgc ggcggccatc aagctctcct atcgtgccga ccggaaggcg
      961 aatcccaatg ccgcattgat tcccggcggg cgactcggag tttggctggt gggcgtgttg
     1021 ggatttgcgg tggtggtggg cgggatcgcg ctgtccttca ttcctcccgc tgaagctacg
     1081 aacaaatggc ttttcgaagc aaagctaatt gcagggaccg tcggaggagt gctcttgggc
     1141 ttgattctct acgttcgcgg cgcgcgagcc aaggcgcgcg aagctgcccg cgctggattc
     1201 accgaaaccc gccactagcg cgcctcgtgc aaattcacgg atccgtgcta cagcgaggag
     1261 agattcttcg actgcatgtc ccggcgcttc gcgcaaatcc gaaaatgcgg aacactccgc
     1321 tcagaatgac gcagccagct tggctaattg atgttcagtt tggattgggt gctggggaga
     1381 tgctgatcga ggtcgggcag gcactcgact tcggtgatgt cggtatcgtc ttcgcgattc
     1441 cagtgtttgg tgctggtcca ttcgccgtcc cagttcttcg gatcaagtag ggtgtatttg
     1501 atttccagag tcttgccgcc gttctgcata cgaatgcgct cgatgatgtg gaagtcgtcg
     1561 ctcacgggaa cgcccacgtc gatccaatgc cgggcggtgg tcatgctcgt ggtatcgact
     1621 actagggtgt cgtcttccca gtgtccgacc gagtcgccat cgtatgtgcg aaccaccaca
     1681 tccggcgctg tatgttttcg cccgtcgata tagatcagtc gaatctcgtt ttcaaatccg
     1741 ctgaccatgt aaatcactgt aggcgtttgg atcatcgcga tcggccagac gcgcgtcata
     1801 atcataggca tgccggccgg atagcatttg ccgatgtcgt cgtggaatgc gtggccggtg
     1861 gccgtggctt tctttccttc ttcgaatagc gcttccgctg cagggtgaaa gtgcggatag
     1921 ggaggtccga acataaacga gttaaagccg gaactgagat cgaccatcca cgtgcccgtt
     1981 aaattgaatg gcggcttcgg gcgcggctta ttcagattgt ccggcgcgag cgcgccttcg
     2041 ggaaacacgt cctcttgctt gttttgatcg gccttgtttt gattagcttg gttttgcccc
     2101 tgcgcgtgca cgaccgtccc aagcgcgccc aaactcacgg ccgccagggc gacactcagc
     2161 gcgaacttca tcttcgaatt cgtcattttg cggttccttt cgcgcggggt tggcgaagcc
     2221 atggcgcgcg gtgcgagccg tgtcgttcgc cgcttgcaaa actacttcgt cggataggga
     2281 atcatgtcgg tcagcacctg cccatcggcc aatttaacgg acacaaatcg gccgcccttt
     2341 tcgccgctgc gcaatgggtg gatcgtcacc gtcacttgat cgcccggctt cagagtggtt
     2401 gaacgccatc cggtgcgcgc catcgtattt gggctgttgc actcgacgcc ccaccggtcc
     2461 gtgccgccat ttgcatttgg cacgtcaatc gagatccaac tgtgaggatt cgaccagctg
     2521 aattggacaa tggttcccac gagctccact tttttgtccc gatcgaacat cgcggcggag
     2581 tgatgcgcga aagccgaacc cgcaaatgca aacaccaggg cgaccgcgaa tgcaattcgt
     2641 gaagttttca tcggctcgct cccactcttt gatttcatcc agccaatatg atccgccgcg
     2701 gtcgtcagtc tgatttacta tgt
//
LOCUS       DATNDT010000054         3213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3936101, whole genome shotgun sequence.
ACCESSION   DATNDT010000054 DATNDT010000000
VERSION     DATNDT010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3213
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3936101"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..793)
                     /locus_tag="VN788_01650"
                     /pseudo
     CDS             complement(38..793)
                     /locus_tag="VN788_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682388.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
     gene            1440..1787
                     /locus_tag="VN788_01655"
     CDS             1440..1787
                     /locus_tag="VN788_01655"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HXU19267.1"
                     /translation="MTERPRIIDWRSKIDAIDTAMLQLLSLRTELALEVGRLKADEGV
                     ALRVPAREQEILSRMKSLNPGPLANDSVAKIYEVILRESIQTQQQHGYGATADGKRSG
                     RMGRANHRSMSRR"
     gene            1798..2670
                     /gene="pheA"
                     /locus_tag="VN788_01660"
     CDS             1798..2670
                     /gene="pheA"
                     /locus_tag="VN788_01660"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HXU19268.1"
                     /translation="MKIEENARVAFQGERGAFSEEAAVKLVGENIMLVPRPTFEAAFR
                     AIDDAAADYILAPIENSLAGSVHRSFDLLVDSRLNILAEVIIPIVHNLIVAPGGSFDD
                     LAIVESHPVALAQCEQFFGAHPRLKRIATEDTAGSVREIVRAGDRSRAAIAGRRAAEI
                     YGGVILQEHLEDNRENYTRFLLLSPSSAEPENSDKLSLVFQLDHRPRALYHALEPFAR
                     RNLNLMKIESRPVHGSPWQYRFYLDLQASRRDPEVAAALRELEKLVVELRILGSYMSA
                     RRSDSNKSQPSART"
     gene            2674..>3213
                     /locus_tag="VN788_01665"
     CDS             2674..>3213
                     /locus_tag="VN788_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXU19269.1"
                     /translation="MIFNMLGDATDQQINHIVDRIKECGFEAHLIRGKERTVIGVVGN
                     SNRKREELLALSSAPGVEEIVRISHPFKLAARSCRPEGSQLDLGQGVIIGGSAVVVAA
                     GPCAVESPDQIADIAARVAKSGAKLLRGGAFKPRSSPYSFQGLGEEGLRLMRDAADKN
                     DLLVISEVMDPSQIQIMLPY"
BASE COUNT          716 a          914 c          923 g          660 t
ORIGIN      
        1 cagtggcagc tacggaaaca atcgcatttt cgagctaggc gtgagtgatg atttgtctcg
       61 ggcggatgtc gtccttcaga agctgcatca cctgtttccc gcagtcaaac gcaatttgcg
      121 ctttgcgcgg gtccactgcc ggcacttcgt tcgcgcccat cggcgaaatg ccgagaaaag
      181 tgattgccgt cgccatggtg ttagcggtga attggccgcc acaagccccc gctcctgggc
      241 acgcgtgatc ttccagttcg catacttcct tctcggtcat tttacctgct gagaaagctc
      301 cgggagcctc gaacacgtct tgaatagtca cgtcgtgccc ctggaatttc cccgccatga
      361 tggagccgcc gtagagcgaa aggccgggca gatcgagccg cgcaagggcc atcaccgtgc
      421 ctggaatggt tttgtcgcag ccgcaaattg cgactaccgc atcgaacgca tgcgaacgca
      481 caaaaagctc gatggaatcc gcaatcactt cgcgcgatac cagcgaacct ttcatgcctt
      541 cggtccccat ggtgattcca tcggaaatca cgatggtatt gcattcgatc ggcgtgccac
      601 ccgcagctcg aatgccttcc ttcaccttct ccgccagcga acgaagatga agattgcacg
      661 gcgtgatttc cgaccaggta ttcgctacag ccaccagtgg ccgcgccaga tcggcatcag
      721 tgagccccgt cgccttcagc atcgcgcggt tcggagcacg cgcaattcct ttttttatca
      781 cgtcactctg cattgctact tggcctcttt cgcgagtgca acaaaaaacc ccgctacctg
      841 gcttccggtg cggggcccac atgcctaagc ttggcttagg atggcggtcc ccgcctggct
      901 actacatata cgaaaatcgg aatcggcagc gtcatagagt aacgtctcac ttaacaatcg
      961 acaattagtt ttctggagcg aaagtaccgg gcggctcagc accgccagtt acgccctgca
     1021 agcccgatta ggctggttcc tgtctgataa gtcaaatttg gaatcattgc aagctgtata
     1081 aattgcactt atgcctgacc cctctaagaa gcgcttgacg gtccaggtaa gcgttgggta
     1141 tagttgcgct tcaattcagg agcgttagac cgcgcccatg agcaccgcta atcgaaattt
     1201 cgtctttacc taccgcttcc tctttttggg gaagcgggcc ggcgaaacgc gcgcggatgg
     1261 aatgaatagc taaaaaccgc acaaagtttc aaggaccggg ccgcgactcc gaacggggac
     1321 gcggcctttt ttgttggcgg aaggatttgt gggaccgaca tacacagggc gcagcgtcat
     1381 ttccagtgtg gcgcgcgata acataagccg actcaacttt gcattttagg agaagcacga
     1441 tgacggaacg accgcgaatc atagactggc gaagcaaaat cgacgcgatt gataccgcta
     1501 tgttgcaact cttgagtctg cgaacggagt tggcgctcga ggtgggacgg cttaaagcgg
     1561 acgagggcgt ggctctgcgt gttcccgctc gcgaacaaga gattctatcc cgcatgaaaa
     1621 gcttgaaccc ggggccgctc gcgaacgatt cggtggccaa aatttacgaa gtgatcctgc
     1681 gggaatcgat tcagacccaa cagcaacacg gttacggtgc aacggcagac gggaagcgaa
     1741 gcggaagaat gggccgcgcg aaccatcgca gcatgtcacg gcggtagaaa aggcaaaatg
     1801 aaaatcgagg aaaacgcgcg agtggcattt cagggtgagc gcggcgcctt cagcgaagag
     1861 gcggccgtga aacttgtcgg cgaaaatatt atgctcgtac cgcgcccgac attcgaagcc
     1921 gcttttcgcg ctatcgacga tgccgccgcg gattacattc tcgccccgat tgaaaacagc
     1981 ctggcgggct cggtccaccg gtcgttcgac ctgctcgtgg atagccggct aaatattctg
     2041 gccgaagtga tcattcccat cgtgcataac ctgatcgttg cgcctggcgg gtcgttcgac
     2101 gatctcgcga ttgtggagtc gcatccggtc gcgctagcgc agtgtgaaca atttttcggc
     2161 gcgcatccgc gactgaaacg gatcgccaca gaggacactg ccggtagcgt acgagagatc
     2221 gtccgcgccg gtgatcgctc gcgcgcggca atcgccggcc gccgcgccgc ggaaatttac
     2281 ggcggcgtga ttctccagga gcatctcgag gacaaccgcg aaaactacac gcgcttcctg
     2341 ctgctatcac cttcgtccgc ggagccggaa aactccgaca agctctcgct ggtatttcag
     2401 ctcgatcacc gccctcgcgc cctttatcac gcgcttgagc cgttcgcgcg gcgaaatttg
     2461 aatctcatga agatcgaaag ccggccggtg cacggaagcc cctggcaata ccgcttttat
     2521 ttggacttgc aagcgtcgag gcgcgatccg gaagtggccg cggcgctgcg cgaactggaa
     2581 aaactggtcg ttgagctgcg gattctcggg tcgtacatgt ccgcaaggcg atcggatagc
     2641 aacaaatctc agccatctgc gaggacctga aaaatgattt tcaacatgct gggcgatgca
     2701 acggaccaac agatcaacca tatcgtcgat cggatcaagg aatgcggatt cgaagcgcac
     2761 ttaattcgcg gcaaggagcg caccgtaatt ggagtggtgg gaaatagcaa ccggaagcgg
     2821 gaagaacttc tcgccttgag ttcggcgccc ggagtcgaag agatcgtccg aatttcgcat
     2881 cccttcaagc tggccgcgcg cagttgccgt ccggagggat cacagcttga cctcggccaa
     2941 ggcgtcatca tcggcggcag cgccgttgta gttgctgcgg ggccatgcgc cgtcgaatca
     3001 ccggatcaga ttgccgacat cgcggctcgg gtggcgaaat cgggggccaa gcttttgcgc
     3061 ggaggcgcct ttaagccgcg ctcgtcaccc tatagcttcc agggattggg cgaggaaggc
     3121 ctgaggctaa tgcgcgacgc ggcggacaag aacgatttgc tggtcataag cgaggtgatg
     3181 gacccatcac aaatccaaat catgctgccg tat
//
LOCUS       DATNDT010000055         2873 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2466875, whole genome shotgun sequence.
ACCESSION   DATNDT010000055 DATNDT010000000
VERSION     DATNDT010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2466875"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VN788_01670"
     CDS             <1..1309
                     /locus_tag="VN788_01670"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU19270.1"
                     /translation="TIVNAPQAVIPYDRASLALDQHGRFKLSAVTGFTKIDAESPELS
                     PINEVLQWASLSDEIVHVRQHGEENDADREETRAKFRKYFAESGMRGFYALPLNDDTG
                     RVGMLCLESKDPDFLTAAHFEILQVLTSQATVALRNAQMYKEVPFISVLEPVLVRKRK
                     FMAIEKRRRRLIVVGTAAVLIFLVAFPFPLRVDGKAIVAPLRRAQIQPETDGVVHKVF
                     VKEGEQVRAGQILAEMEDWNAQAAVAEAQSQYQTALLQKNRALASSEGTVAGMQSVQA
                     QYWKAELDRAQQLLERSQLRSPIDGTITTPHVEDLAGRKLSRGDSFAEVVDTSQAIVD
                     VAADDEDAGLLRVGEPASVKLNSYPERTFHGEVSIISPKATSIQGSPTFYSRVSIPNA
                     DGAIRAGMEGRGKVRVAWRPAGYVLLRRPTAWVYSKLWDWLGW"
     gene            1312..2238
                     /locus_tag="VN788_01675"
     CDS             1312..2238
                     /locus_tag="VN788_01675"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU19271.1"
                     /translation="MARMLTIRKLLSGVIVIFAMQLASCGGDKPQPVMASNAAPLPAA
                     TASSPDPSNAAAADSELVVSGPIIVEHEVDLTAQRDGVVTRIFYDVPAHVKAGTLLAQ
                     FDDRQLQAQLESARAKSRSVEADLNNWKAERKVMQADYTRAQQAAAQGLISAEQLQHA
                     QFQVESHDWDIKRVTELLNNAQDDERSLELELQKTKIVAPFDAVVARRYIRESQSVAK
                     GDRLFWVTSEAPLLVRFTLPEGLFGKVHRGQEFRVDSPDAAGEPHTARVREISPVVDP
                     ASGTYEVLVELAGNPGSLRPGMTASVHLDHMQ"
     gene            2235..>2873
                     /locus_tag="VN788_01680"
     CDS             2235..>2873
                     /locus_tag="VN788_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19272.1"
                     /translation="MNLTRVFDVALPELPARIISDRVPRMPPNIVFREHIEDGKPIVR
                     ATIPPEDAIYRFPKENWELIQLFDGNRSYEEIARAYSAQIGTEYGPEEVREFAAALDE
                     DDFWFKTPQERNVVLMQMDAEKRRKLLKAKKSKWGDLAEISFPAVNPDKFLTWLYRHT
                     SFIYTWWFTLFTFALFAVMAVITIANWGEIGQDTLHFFNFSQKSWADVGVFYV"
BASE COUNT          656 a          771 c          843 g          603 t
ORIGIN      
        1 gacaattgtg aacgcacctc aagcggtgat cccatacgat cgcgcgtcgc tggccctgga
       61 tcagcacggc cgctttaaac tcagcgccgt cacgggcttc acgaaaatcg atgcggaatc
      121 gccggaactc tcgcccataa atgaagtgct gcaatgggct tccttgtcgg atgaaattgt
      181 tcacgtgcgg cagcacggcg aagaaaacga cgcggatcgg gaagaaacgc gcgcgaagtt
      241 tcgaaaatat ttcgcggaat ccggaatgcg tgggttttac gccctgccgc tcaacgatga
      301 taccggacgc gtagggatgt tgtgtttgga aagtaaagac cccgatttcc tcaccgctgc
      361 tcacttcgag attttgcaag ttctcaccag ccaagccacg gtggctctgc gcaacgcgca
      421 gatgtacaag gaagttccat ttatctccgt tctcgagccg gtattggtgc gcaaacggaa
      481 gttcatggcg atagaaaagc gccgtcgcag gctaatcgtc gtcggcacgg cggcggtttt
      541 gattttcctt gtcgcgtttc cgttcccact ccgtgtagat ggcaaggcta tagttgcgcc
      601 acttcgtcgc gctcagattc aacccgagac agacggcgta gtccacaagg tcttcgtcaa
      661 ggaaggtgag caggtacgcg ccggacagat actcgcggag atggaagact ggaatgctca
      721 ggccgcagtc gcggaagcgc agtctcagta ccaaactgca cttctgcaaa agaaccgggc
      781 actcgcatcc agcgagggaa ctgttgccgg catgcaaagc gtccaggcgc agtactggaa
      841 ggctgaactc gatcgcgcgc agcagctatt ggaaaggtcg caacttcgat cgccgatcga
      901 cggaacgatt acgactccgc atgtcgaaga tctcgcgggg cgcaagctga gccgtggcga
      961 ctccttcgcg gaagttgtgg acacgtcaca ggccatcgtg gacgtcgccg cagacgacga
     1021 ggatgcaggg ctgctgcgtg ttggcgagcc ggcttcggtc aagctgaata gctatccgga
     1081 gcgaacattc cacggagagg tttcaatcat cagtccaaaa gctacgagca ttcaaggatc
     1141 gcccacgttc tattcccgcg tttccattcc caatgcggat ggagccattc gagcggggat
     1201 ggaagggcgt ggaaaggttc gtgttgcctg gcggcctgca ggctacgtgc ttttgcgccg
     1261 gccgactgcc tgggtttatt caaaattgtg ggactggcta ggatggtaac gatggcgcgg
     1321 atgctaacca tacgcaaatt gctttccggg gtgatagtga ttttcgcgat gcaactggcg
     1381 agttgcggtg gcgataagcc ccaaccagtg atggcttcca atgcggcgcc actgcctgcg
     1441 gcgactgcga gttcacctga tccgtccaac gcagccgctg ccgattcgga attggtggtt
     1501 tcgggcccga tcatcgtcga gcatgaggtc gacttgacgg cacaacgcga tggcgtggtg
     1561 acgcggattt tctatgatgt cccggcacat gtaaaggcgg gaactttact ggctcagttc
     1621 gatgaccggc aattgcaggc gcaacttgaa tctgcacgcg caaagtctcg cagtgtcgag
     1681 gctgacttga acaactggaa ggccgaaaga aaagtcatgc aggcagacta cacgcgcgcc
     1741 caacaggccg cagcacaggg acttatctcg gccgagcaac tgcagcacgc acaattccag
     1801 gtcgaatctc acgattggga tataaagcgg gtgacggaac ttctgaacaa cgcgcaggac
     1861 gacgagcgct cgctcgaact ggagctccag aagactaaga tcgtcgcgcc gttcgacgcg
     1921 gttgtggcgc gccgctacat acgcgaatcg caaagcgtgg ccaagggcga caggcttttt
     1981 tgggtcacaa gcgaagcgcc cctcctggtc cgattcacac taccggaggg acttttcggc
     2041 aaggtgcatc ggggccagga atttcgggtt gattcacccg acgcggctgg agaaccacat
     2101 accgcacggg tgcgcgaaat cagccctgtc gtggatccgg cgagtggaac gtatgaagtt
     2161 ttggttgaat tggccggcaa ccccggttcg cttcgcccag ggatgactgc gtcggtgcat
     2221 ttggaccaca tgcaatgaat ttgacgcgtg tctttgacgt tgcgctgcca gaacttcccg
     2281 cccggatcat ttccgatcgc gtcccacgca tgcctcccaa tatcgtattt agagaacata
     2341 ttgaagacgg caaacccatc gttcgtgcca cgattccgcc cgaggatgct atctaccgtt
     2401 tccccaagga gaattgggag ctaatccagc tatttgacgg caatcggtcc tatgaagaga
     2461 tcgcgagagc gtattccgct caaatcggca cggaatacgg tcctgaggaa gttcgcgaat
     2521 tcgctgcagc gcttgacgag gatgatttct ggttcaagac gccacaggaa cgaaacgtcg
     2581 tcttgatgca aatggacgca gagaagcgtc gcaaactgct aaaggccaaa aagagcaagt
     2641 ggggagacct cgcggaaatc agctttcccg ccgtcaatcc cgataagttt ctaacctggc
     2701 tgtataggca tacttccttc atctatacat ggtggttcac gctgttcacg tttgcgctct
     2761 tcgcagtaat ggcagtaatc acgatcgcga attggggcga aattggccag gatacgctgc
     2821 acttcttcaa tttctcgcaa aagagctggg cggacgtagg ggtcttttac gtg
//
LOCUS       DATNDT010000056         6397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3679095, whole genome shotgun sequence.
ACCESSION   DATNDT010000056 DATNDT010000000
VERSION     DATNDT010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6397
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3679095"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1146
                     /locus_tag="VN788_01685"
     CDS             <1..1146
                     /locus_tag="VN788_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HXU19273.1"
                     /translation="SKPRPATFEFPRELMKEEQPKYDFQTQEERRLFYVALTRAQKRL
                     TLSTVVNRWKRESPFVIDCLANPKIQKFDTEQCQPKVEVPPVEETTGPLWESQDEPPL
                     FKAARENLKAYSRVPLWAKAYHPPQPEPLQLSASRIDAYDRCPMKYMFQYVWNIRGGP
                     NAQKTFGNVMHSTIREFVGEMRKGSAVPFEEVLAIYERQWSSAGFPDDYQQGEYRKAG
                     REQLEAFHHSCYDAPPDVLYQEKKFELPLDHDIIVTGRMDQVNRLDAGEVEIVDYKTG
                     SPRDEKKAAENLQLSIYALAAREVLDLEPTRLVLYNLTDNQAVATTRDAKALAAAKEK
                     IADVADQIRAGNFRAKPGFGCGYCDFKPLCPEHEQLIAIQLAVNATR"
     gene            complement(1510..2592)
                     /locus_tag="VN788_01690"
     CDS             complement(1510..2592)
                     /locus_tag="VN788_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU19274.1"
                     /translation="MMRRLSLLGTLLLFGFTGQAWPQAQRPTPPGTPAQRTPAQQQNP
                     PRIIVPTYTVIVPVTVKDDHGQLVGDLQKNDFRVFSDGVEQKIRQFSSEPIPLSAVVL
                     IDNDLDQRQASQVQKSLTAISAGFGPPDEVAVVTYDQFPETVADFSFNNDHLFTQLKR
                     LDLESHNSAVIADPTTAGPMMNGHQPVDTGRGIPQHGSKRYSNDNALDDAVFAASNML
                     KDRGRDRRKIIFLISDGTNAKNEHTFDQTLHSLLANDVAVYSISVTHSLPIGKSLIQR
                     GASELEKYALNSGGDTFYASKQPELERLYSDVTEQARNQYTLTFSPENIPKGQDFHEI
                     EVRVERPGLNVETRRGFYLSAISAGH"
     gene            2729..3802
                     /locus_tag="VN788_01695"
     CDS             2729..3802
                     /locus_tag="VN788_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU19275.1"
                     /translation="MRLTSIAAGIVVLAASTYPLARPMASHASAAPSESALQESGSRG
                     PLINPSRPSQEPETAQQKEPPQSSAPLQVQTTVVNVFTTVRDRHNGIVSDLTKNDFKV
                     FEDGAEQKISYFTKEVDLPLSLALLMDTSGSMQDILNAEKEAASNFVEEVMHKKDEAA
                     VLSFDTDVNLLADFSEDPGVLQHAIRRAEINVDATGVGGTGGTVPSRGGGTNLYDAVY
                     LAAHDELSSEAGRKAIILLTDAEDTGSKLSENDAIESAQRADAVLHFILIIDPSASEG
                     YGPGVASKMAHETGGRVISVRNDEGLRKAFDTIAEELRSQYVIGYYPANTKHDGTFRK
                     IRVDVDRADLKILARKGYYAPSR"
     gene            complement(3843..4928)
                     /locus_tag="VN788_01700"
     CDS             complement(3843..4928)
                     /locus_tag="VN788_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014006768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXU19276.1"
                     /translation="MKSWLRIVLISAICGFAGGKTLVAQQTAPQEGDYIARNFVFHSG
                     ETLPELRLHYTALGKPVRDAQGRVTNAILILHGTGGTGRGFLQPIYAGELFGPGQLLD
                     ASKYYIILPDGIGHGKSSKPSDGLHAHFPHYDYDDMVAAQHLLLTKGLDVNHLRLVTG
                     TSMGCMHSFVWGETYPDFMDALMPLACLPTQIAGRNRLWREMAMVAIKKDPEWKGGDY
                     IAEPQQALRSWINLLIIAGSAPLQMQKTLPTRHAADEYLEKAYETRVKDLDANDVLYQ
                     LDASRNYDPSGELEKIVAPVMWVNSADDFINPPELGIADKEVKRIKNGKFVLLPISDQ
                     TYGHGTHTHAAAWKQYLAELLKESEPN"
     gene            complement(4958..6037)
                     /locus_tag="VN788_01705"
     CDS             complement(4958..6037)
                     /locus_tag="VN788_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU19277.1"
                     /translation="MTNPPRSLPIAVFVLLTLFAVCGVCARAQEAPSKPAGPATSSPQ
                     QAPATPSPQTAAPPPQQPPIVSTTGLVHLVVTVVDRHHDFITDLDKTDFKVLDNGSPQ
                     VIRFFGRETDLPLRIALLLDTSNSIRPRLKFEKDAAIDFLDKVIRRDKDLAFLMTFDN
                     EPQVIQDYTSSVDLLTSAIQQQRAGGGTALNDAIYMAAEKLAHPPVPVGPETEVRRVI
                     VVISDGDDNLSDRALSDAIDASIRSEVAVFTVSTSTDWLAIDDASRPSKYHVEGGDKV
                     LQEFADQTGGHAFFPYRVDDLAQSFLDIGTELRSQYFIAYAPANPPPSGVYRKIDVKT
                     DRKGLTVRTRKGYYAAISSGAPAAQ"
     gene            complement(6057..>6397)
                     /locus_tag="VN788_01710"
     CDS             complement(6057..>6397)
                     /locus_tag="VN788_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXU19278.1"
                     /translation="LLRLKGHTFIEASLYTGRTHQIRVHFSALGHPVVGDTLYGAPRQ
                     ERAASALLPPLNRNFLHAARVAFVHPRTGRRIEVRAPLPPELVDYLAFLARSVQVDPH
                     SVDAALREFL"
BASE COUNT         1419 a         1647 c         1961 g         1370 t
ORIGIN      
        1 tcgaagccgc gcccggccac gtttgaattt ccgcgagaac tcatgaagga ggaacagccg
       61 aaatatgatt ttcagacaca ggaggagcgg cggctatttt atgtggcgct gacgcgcgcg
      121 caaaagcggc tgacgctttc cacggtcgtc aatcgctgga aaagagaatc gccgttcgtg
      181 attgattgtc tggcaaatcc gaagattcag aaatttgaca ccgagcagtg ccagccgaaa
      241 gtagaagtgc cgccagttga agaaaccacc gggccgttgt gggagtcgca agatgaacct
      301 ccgctcttta aagcagcacg agaaaaccta aaggcatatt cgcgcgtccc gttgtgggcc
      361 aaggcctacc atccgccgca gcccgaaccg ctgcaattga gcgcgtcaag gattgatgcg
      421 tacgaccgat gtcctatgaa gtatatgttt cagtacgtgt ggaatattcg cggcgggccg
      481 aatgcacaga aaacatttgg aaacgtgatg cattcgacga ttcgggaatt tgtgggcgag
      541 atgcgcaagg gatcggcggt tccgttcgag gaagtgctgg caatttacga gcggcagtgg
      601 tcgtcagcag gttttcccga tgattatcag caaggggaat atcgcaaggc gggccgcgag
      661 caactagaag cctttcatca ttcttgttac gatgcgccac ccgacgttct ttatcaggag
      721 aaaaagttcg agctgccgct cgaccacgac attattgtga cgggacggat ggaccaggtg
      781 aatcgtctgg atgccggcga agtggagatt gtggattaca aaaccggaag tccgcgcgac
      841 gaaaaaaagg ctgctgagaa tctacaacta agtatctacg cgctggcggc gcgcgaggtg
      901 ttggacttgg agccaacacg tctggtgctc tacaacctta cggacaatca agccgtagcg
      961 actacgcgcg acgcaaaagc tctcgccgcg gcgaaggaaa aaatcgcgga tgtggccgac
     1021 cagattcgcg ccggaaattt tcgcgcgaag cccggttttg gttgcggata ttgcgatttt
     1081 aaaccgcttt gtcctgaaca cgaacagttg attgcgattc agctcgccgt gaacgccacg
     1141 cgctagcaag ccccgctggc tgcagatacc acgaatgaaa gaggacggca cctttggtgc
     1201 taggtgaatg gcagccaagt atcttcgccg ggcacggcat gccgtgcccc tacagataca
     1261 aaacggcgcg cgacaaaata aaaagcggga caatcgtttt accgattgcc ccgctttatt
     1321 tcttgccagg cttttcgatt caaagcctgg agtgggttcc gcgcatttac ttaccggaac
     1381 tttctccgcg cgacggcgcc gaattttata ttcggcagcc gatcccgctt ggttcatcta
     1441 tagaagcagg aaccgatgaa gtcaaggaga aaaacttggg cgcgattgtc gttttgaaaa
     1501 tgttcgctgc tagtgacccg cgctgatggc gctcaagtaa aatccgcggc gagtctcgac
     1561 gttcaagccg ggccgctcaa cgcgcacttc gatctcgtgg aagtcctgac ccttgggaat
     1621 attctccggc gaaaaggtga gcgtgtattg gttgcgcgct tgttcggtga catcggaata
     1681 aaggcgctca agttcgggtt gcttcgatgc gtaaaaggta tcgccgccgc tattcaacgc
     1741 atatttttcg agctctgaag ctcctcgctg aataagggac ttaccaatcg gcagcgaatg
     1801 cgtgacggaa atggaataca ctgcaacgtc gttggcgagg agggagtgaa gcgtctggtc
     1861 gaaagtgtgc tcgttcttgg cgttggtgcc gtcggagatc agaaagatga ttttgcggcg
     1921 gtcacggccg cgatccttga gcatatttga cgcggcgaag acggcatcat caagagcatt
     1981 gtcgttgctg tatcgctttg acccgtgctg cgggatgccg cgccccgtat ccacgggctg
     2041 atggccattc atcatgggtc cggcggtggt agggtcggca atcaccgcgc tattgtggga
     2101 ttcgagatct agacgcttta gttgtgtgaa caaatggtcg ttgttgaaag aaaagtcggc
     2161 cacagtttcg ggaaactggt cgtaggttac cactgcaact tcatcgggag ggccgaagcc
     2221 ggcggagatg gccgtgagac tcttttggac ctgcgaggct tgcctctgat cgagatcgtt
     2281 gtcgataaga acgacggccg aaagcggtat gggctcggag gagaactggc gaatcttctg
     2341 ttcgactcca tcagagaaga ctcggaagtc attcttttga agatccccca ccagttgacc
     2401 gtggtcatct tttactgtta ccggaacgat gacggtgtat gttggaacga tgatgcgtgg
     2461 cggattctgc tgctgcgccg gagttctttg cgctggagta ccgggaggcg tcggccgctg
     2521 agcttgtggc caggcttggc cggtaaagcc aaacaggagg agcgtaccta gaagtgatag
     2581 tcttcgcatc atctatgcgt ctcgtttctc cgcagcctag gctgcgagag tcttgcgggg
     2641 acctgggcgc gggtctttgg gccggaacat ccgtaagagt gctcggcgca tcgtatcagt
     2701 ctagttcctt cctcggagga attatacaat gcggctgacc agcatcgcgg cgggtattgt
     2761 ggttttggca gccagcacgt atccgctggc gcgaccgatg gcctcgcacg cgtccgccgc
     2821 gccgagcgag agtgcgctcc aagaatcagg ctcgcgaggg ccgctaatca acccttcccg
     2881 acccagccaa gagccagaga ctgcccagca gaaggaaccc ccgcaatcgt ccgcgccctt
     2941 gcaagtgcag acgacggtgg tgaacgtgtt tacaactgtt cgcgaccggc acaacggaat
     3001 tgtgtccgac ctgacgaaga acgatttcaa agtatttgaa gacggagcgg agcagaagat
     3061 ttcctatttc acgaaggaag tcgatctgcc actttcactt gcacttttaa tggataccag
     3121 cgggagcatg caggacatct tgaacgccga aaaggaagcg gcgtcgaact tcgtcgaaga
     3181 agtgatgcat aagaaggacg aagcggctgt gttgagcttc gataccgacg tgaatctact
     3241 ggcagatttc agcgaggatc cgggggtgct tcagcatgcc atccggcggg cggaaattaa
     3301 cgtggatgcc acgggcgtag gcgggactgg gggcacggtg ccgtcacgtg ggggcggcac
     3361 gaatctttat gacgcggttt atctggcggc gcacgacgaa ctatcgtccg aagcgggacg
     3421 taaagccatc atcctgctga cggacgccga ggacaccgga agcaaattga gcgagaacga
     3481 cgcgatcgaa tccgctcagc gcgccgatgc ggtgctccat ttcattctga ttattgaccc
     3541 cagcgcctcg gaaggatatg ggcccggcgt ggcctcaaag atggcgcacg aaaccggtgg
     3601 acgcgtgatc agcgtgcgca atgacgaggg acttcggaaa gccttcgata cgatagccga
     3661 ggaactgcgg tcgcagtacg ttatcggcta ctatccggcg aatacaaagc atgacggcac
     3721 gtttcgaaag atacgggtgg atgtggatcg cgccgaccta aagatcctgg ctcgcaaagg
     3781 ctactacgcg ccatcccgat agcgcacgta tcgaccctga agagcgcggg tagagtgaaa
     3841 gcttagttgg gttccgactc tttcagcagc tccgccaaat attgtttcca tgccgccgcg
     3901 tgcgtgtgcg tcccgtgacc gtatgtttgg tcgctgatcg gcagcagcac aaactttccg
     3961 ttcttgattc gcttcacttc cttgtccgca atgcccaact cgggcggatt gatgaagtcg
     4021 tccgctgaat tgacccacat caccggagcg acgattttct ctagctcgcc ggaagggtcg
     4081 tagttccgcg aggcatctaa ttggtacaaa acatcgtttg catcgaggtc cttcactcgc
     4141 gtctcatacg ctttctcgag gtattcatcg gcggcatggc gcgtaggcag cgtcttctgc
     4201 atttgcagag gcgcgcttcc ggcgattatc agaagattga tccagcttcg cagcgcttgt
     4261 tgcggctcag caatgtagtc gccgcccttc cattcgggat cctttttgat cgcaaccatg
     4321 gccatctcgc gccacaagcg attcctgccc gcaatctgcg tgggcaggca ggccagcggc
     4381 atcagcgcat ccatgaagtc cgggtacgtt tcgccccata caaacgaatg catgcagccc
     4441 atggaagtgc cggtgaccag gcgcaaatgg ttcacgtcca gtccttttgt tagcagcagg
     4501 tgctgcgcgg ctaccatgtc gtcgtaatcg taatgtggaa aatgcgcgtg gagtccatca
     4561 cttggcttgg aagatttgcc gtgaccgatt ccgtcgggaa gaatgatgta atacttgctc
     4621 gcatccagaa gctgtcccgg tccgaatagt tcacccgcat aaatcggctg caaaaatccg
     4681 cgcccagtac cgccggttcc atgcaagatg agtatcgcgt tagtcacgcg tccctgcgca
     4741 tcgcgcaccg gttttccaag cgcggtgtag tgcaggcgca gttcaggcag cgtttctccc
     4801 gagtggaaca cgaaattcct agcgatgtag tcgccttcct gcggagccgt ctgctgggca
     4861 accaacgttt tgcctccggc aaacccgcag atagcgctaa tcaagacaat tcttagccag
     4921 cttttcatgg cgtaggctcc cctcgttccc tgagcggcta ttgggccgca ggcgccccag
     4981 aagatatggc ggcgtagtag cctttgcggg tgcgaacagt gagacctttg cggtcagttt
     5041 tgacgtcaat cttgcggtac acgccagatg gaggcgggtt ggcgggagcg taagcaatga
     5101 aatactggct acgcagctcg gttccgatgt cgaggaagga ctgagcgaga tcgtcgacac
     5161 ggtagggaaa gaaggcgtgg ccgccggttt ggtccgcgaa ttcctgcaag accttatctc
     5221 ctccctcgac gtgatatttg ctgggacggc tcgcgtcatc gattgcgagc cagtcggtgc
     5281 tggtgctgac cgtgaagacg gcgacttcag agcgaatgga tgcgtcgatt gcgtcgctaa
     5341 gcgcgcgatc gctcaggttg tcgtcaccgt cgctaatgac aacgatgacg cgccgcactt
     5401 ccgtttccgg accgaccgga acgggtgggt gcgccagttt ttcggcggcc atatagatgg
     5461 cgtcattcaa agccgtgccg cctccggcgc gctgctgctg aatggccgag gtgagcagat
     5521 cgacgctgga ggtgtaatcc tgaatgacct gtggctcgtt atcgaatgtc atcaggaagg
     5581 cgagatcttt atcgcgacga attactttgt cgaggaaatc aatcgcagca tctttttcaa
     5641 atttcaggcg cggccgaatg ctattggacg tgtccagaag caacgcgatc cgcaaaggca
     5701 gatcggtttc gcggccgaag aaacgaatga cttgggggga accgttatcg agaaccttga
     5761 aatcggtctt atcgagatcg gtgatgaagt cgtgatggcg atccacaacc gtaacgacga
     5821 ggtggacaag gcccgtggtg ctgacgattg gtggctgctg aggaggtggt gcggccgttt
     5881 gtggactggg tgtagctggg gcctgctgag gggaagaagt ggccgggcct gcgggtttgg
     5941 atggagcctc ttgcgcacga gcgcaaaccc cgcagaccgc gaatagggtg agcagcacaa
     6001 acactgctat cgggagggag cgaggaggat tcgtcacgct gcacttcacg gtagaattat
     6061 aggaattcgc ggagggcagc gtcaaccgaa tggggatcaa cttggacgct acgggcgaga
     6121 aaggctagat agtccacgag ctcgggcggg agcggcgcac gaacttcgat tcgccgacct
     6181 gttcgtggat ggacgaaggc gacgcgggca gcatggagga aattgcggtt tagcggtggg
     6241 agcagcgcag aggcagcgcg ttcctggcgc ggggcgccgt agagcgtatc gcctacaacc
     6301 ggatgcccga gcgcagaaaa atgaacgcga atctggtggg ttctgccggt gtacaagctg
     6361 gcttcgatga aggtgtggcc ttttagccgg agtagaa
//
LOCUS       DATNDT010000057         5947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1350499, whole genome shotgun sequence.
ACCESSION   DATNDT010000057 DATNDT010000000
VERSION     DATNDT010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5947
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350499"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1484
                     /gene="nagA"
                     /locus_tag="VN788_01715"
     CDS             291..1484
                     /gene="nagA"
                     /locus_tag="VN788_01715"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HXU19279.1"
                     /translation="MPATVIHASRILTPEEEINDGVIVVEDGRIAALGHRDEVRVPAG
                     AIDYVAAGMTVVPGFVDLHIHGAGGHDVMEANARALDRITSTVARHGTTSIVATTVTA
                     PIEDTCHTLEGIARYIRSLENPEDPPKPGRVAAEILGIHLEGPFISPARRGVHPVESI
                     VKPSVEILDRLLKASDGFVRILTIAPEMPGAMDVIRAAVAAKVTVGLGHTDANYDQAR
                     AAIQAGARHAVHIYNAMRPFTHRDPGIVGAIMTDPEISAEVIADGVHVAGPAIQVLIG
                     TKGFDTVLLVSDGIAATGMRDGNYRLGNFEVNVRNGICRNAEGKLAGSTLTLDRALRN
                     IVELGVPLRDAVRMATVLPARRLGLAGKKGIIAVGADADLVALTDDLRVAGVMTGGVG
                     LAAQS"
     gene            complement(1481..1747)
                     /locus_tag="VN788_01720"
     CDS             complement(1481..1747)
                     /locus_tag="VN788_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HXU19280.1"
                     /translation="MANTELTVYSTTWCCDCRRVKQFLQERQVTFREVNIDEAPEAEE
                     LVLRVNNGRRKVPTIELEGRYFACSPFDPHQLADELKIPLNPRS"
     gene            complement(1847..2281)
                     /locus_tag="VN788_01725"
     CDS             complement(1847..2281)
                     /locus_tag="VN788_01725"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HXU19281.1"
                     /translation="MLPQSIELQVVTPERHVLQADVQAVEIPGKEGYLGILPGHAPLI
                     TELGIGILAYRKESETRYLTVIHGYAEVLPDRVIVLAEISERGEEIDPQRSRAAHDRA
                     QAELAKTGAGTPEWEREKLAIERAAVRLEAVGKMTGAPKAHS"
     gene            complement(2308..3759)
                     /gene="atpD"
                     /locus_tag="VN788_01730"
     CDS             complement(2308..3759)
                     /gene="atpD"
                     /locus_tag="VN788_01730"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HXU19282.1"
                     /translation="MASDNAKNGHVVEVIGPVVDVQFEEHHLPRIHNAVRIWSEGFDI
                     PEPMDIVAEVAQHLGEDRVRCVAMKPTEGLVRGMKAQDLGEPISVPVGRGTLGRVLNV
                     LGDPVDGFGPVKADKRYPIHRSAPSLEDQSTNLEMFETGIKVIDLIEPYLKGGKIGLF
                     GGAGVGKTVLIQELINNVAMKHGGVSVFAGVGERTREGNDLWLDFQESGVIEPGNPDK
                     SRCALIYGQMTEPPGARLRVGLTGLTVAEYFRDEEHLDVLLFIDNIFRFVQAGSEVSA
                     LLGRMPSAVGYQPNLNTEIGELQERITSTKSGSITSVQAIYVPADDYTDPAPAATFSH
                     LDATTNLSRQIVERGIYPAVDPLASFSRILDPRILGQEHYSVARSVKSVLQRYKELQD
                     IIAILGIEELSDEDKQTVARARKIERFLSQPFFVAQQFSGLEGKYVKVEDTICGFKEI
                     VEGKHDDLPEQAFYMVGTIEEARAKAEKIAATA"
     gene            complement(3835..4776)
                     /gene="atpG"
                     /locus_tag="VN788_01735"
     CDS             complement(3835..4776)
                     /gene="atpG"
                     /locus_tag="VN788_01735"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011345412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HXU19283.1"
                     /translation="MPTLLDYRRRIRSVKNTQQITRAMKFISAAKLRRAQEGVFAARP
                     YAKEIIRVLRSAAARMESTAHPLLERRPEERILLVVMTGDRSLAGAFNSNVIRRTIAF
                     LRENASKKNQLIAIGKKGRDTLRKRGWSTVGEYLNVSSKVEFSTAKEIANRIADLYAS
                     REVDAVYAVYNEFKNVMVQKLITQKLLPIDPDVLASPSEEAGNTSTGTRRDAEASLPH
                     DRYPSGQSLVDYIYEEPVDEIFGGLVPRYLESQVFRILLESAAAEHAARMTAMDAATR
                     NASELIDKLTLQMNKIRQAGITKELIEIVSGAASAMS"
     gene            complement(4795..>5947)
                     /gene="atpA"
                     /locus_tag="VN788_01740"
     CDS             complement(4795..>5947)
                     /gene="atpA"
                     /locus_tag="VN788_01740"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXU19284.1"
                     /translation="PGVVDRQPVREPLQTGIKAIDSMIPIGRGQRELIIGDRQTGKTA
                     IILDTILNQRGGDMICIYCAIGQKRSTVAQVVKTLSDHGALDYSMVVAASAAEPASMQ
                     YIAPFAAAAMGEFFRDNKRHAICFYDDLSKHAQSYREISLLLRRPPGREAFPGDVFFL
                     HSRLLERAAKLNDKLGAGSLTALPVIETQAGDVSAYIPTNVISITDGQIYLEADLFNA
                     GIRPAINAGISVSRVGGNAQIKAMRQVAGTLRLDMAQYRELAAFTQFGSEQLDKTTQA
                     QLARGQRLMEILKQDQYQPLPVEKQVLLLFASSNRYLDDIDVSECRRFELEMYTFVET
                     NHPGVLQAIRDKKALDDAIRAEATKALDSFKERFRASLPESHQAATAAR"
BASE COUNT         1252 a         1615 c         1763 g         1317 t
ORIGIN      
        1 agttttccct gttcatactg ctttaccttg gctttggccg ctttgcgccg gcggcgctgg
       61 cgcttctgag ccacatagcg tttttcattt gccatcgatt ccctccgaac tgggcgaagg
      121 ttgattgtag cggagaaggc aacggatgca agctccgctg tgattttgcg ccgcaatccc
      181 gctgccagta tgctagagga attgcggcgc aaaatcaagc tccgctgtga ttttgcgccg
      241 caattcctct agcatactgg cagcgggatt ttggacaggc gagggcaaag atgccggcca
      301 ccgtcataca cgcgagccga atattgaccc ctgaggaaga gatcaacgac ggagtaatcg
      361 tcgttgaaga cggccgcatt gcggccttag gacatcggga cgaggttcgc gttcccgcgg
      421 gcgcgattga ctacgtggcg gcgggaatga ccgtggttcc gggctttgtg gatctgcata
      481 ttcacggcgc cggcggacac gacgtaatgg aggccaatgc ccgcgcgcta gaccgaatca
      541 cctcaaccgt cgcgcggcat gggaccacgt ccatcgtggc aaccacggtt accgcgccga
      601 ttgaagatac gtgccatacg cttgaaggaa tcgcgcgcta cattcgcagc cttgaaaatc
      661 cagaggatcc gccgaagccc ggccgggtgg cggccgaaat cctcggaatt catctggagg
      721 ggcccttcat cagcccagcc cgccggggcg tacatccagt tgaatccatc gtcaaacctt
      781 cggtggaaat tctcgatagg cttctaaagg cttccgatgg gttcgttcgg attttgacga
      841 tcgcgcccga aatgcccggc gcaatggatg tgatcagggc ggcggtagcg gcaaaagtta
      901 ccgtggggct tggccatacc gacgcaaact acgaccaggc gcgcgcggcg attcaggcag
      961 gagcacgcca cgcggtgcac atttacaatg ctatgcgccc attcacgcat cgcgatcccg
     1021 gcatcgtggg cgcaataatg accgatccag aaataagcgc ggaagtgatt gcggacggcg
     1081 tacatgtggc cgggccagcg attcaggtac taattgggac aaagggattc gacaccgttt
     1141 tactggtgag cgacggcatc gcggcgacgg gaatgcggga tggcaattac cggttgggga
     1201 attttgaagt gaacgttcga aatggcatct gtcgaaatgc ggagggaaaa ctagccggca
     1261 gcacgctgac gttggatcgg gcactgcgga acatcgtgga actgggtgtg ccgctgcgcg
     1321 atgcagtgcg gatggccacc gtgctaccgg cgcggcgact gggtctagct ggcaaaaagg
     1381 gcatcattgc ggtgggagcg gatgccgatc tggttgcgct gactgatgat ctgcgcgttg
     1441 caggggtaat gacaggcggc gtggggctgg cagcgcaaag ctagctacga ggattcaacg
     1501 gaattttcag ttcatctgcg agctgatgtg gatcaaacgg actgcaggca aagtatcgcc
     1561 cttctagctc aattgtagga actttccggc ggccattatt gacgcggaga accaactctt
     1621 cagcttcggg agcttcgtcg atattcactt cgcggaaagt tacttggcgc tcttggagaa
     1681 actgcttcac acgccggcag tcgcagcacc aagtggttga atagactgta agctcggtat
     1741 tagccatgat tacgccaagt agccaagaaa ctatcttact atttaatgca aaggtgaaca
     1801 cggagatttt agtcgcgatt tgctttggcg cggatgcgtt ggcgatctag gaatgagctt
     1861 tcggagcgcc ggtcatcttt ccaacagctt ccaggcgcac cgcggcgcgc tcgatggcga
     1921 gtttttcgcg ttcccattcc ggagtgcccg cgcctgtctt agccaactcc gcttgcgcgc
     1981 gatcatgagc agcgcgagat cgctgggggt caatctcctc gccacgctcg gaaatttcag
     2041 cgagaacgat cacgcggtct gggaggactt ccgcataacc gtggatgacc gtgaggtaac
     2101 gcgtttcgga ctccttgcga taggccagga ttccgatgcc gagttccgtg atgagcggag
     2161 cgtgaccggg caaaatgccg agatagcctt ccttgcccgg tatctcgacg gcctgcacat
     2221 ccgcctgaag aacatgacgc tcaggcgtga ccacttgcag ttcgatactt tgaggtagca
     2281 ttcattcaac cttgatttgg gcgacgacta cgccgtggcg gctatttttt ccgccttggc
     2341 gcgcgcctct tcgatcgtac cgaccatgta aaaagcctgc tcgggaagat cgtcgtgctt
     2401 gccttccacg atttccttga agccgcaaat cgtgtcttcg acttttacgt acttgccttc
     2461 gagaccgctg aactgctggg cgacgaagaa aggttgggaa aggaaacgtt cgatcttgcg
     2521 cgcacgagcg acagtttgct tatcctcgtc ggagagttcc tcgatgccca agatggcgat
     2581 gatgtcttgc aactccttgt agcgttggag cacggatttc acggagcgcg cgacgctgta
     2641 atgttcctgg cccaaaatgc gaggatcgag aatacgcgag aagctggcca gtggatcaac
     2701 ggcgggataa attcctcgct cgacgatttg ccggctcaag ttcgtggtcg cgtccaagtg
     2761 gctgaaagtt gccgcgggag cggggtcggt gtagtcatcg gcggggacgt aaattgcctg
     2821 caccgacgta atcgagccgc tcttggtgga ggtgatgcgt tcctgcagct ctccaatttc
     2881 agtgttgaga ttgggctgat agccgacggc cgagggcatg cggccgagca gggccgatac
     2941 ttcggagcca gcttgcacga agcggaaaat attatcgatg aagaggagaa catcgagatg
     3001 ttcttcatcg cggaaatatt ctgcgacggt tagcccggtg aggccgacgc gaagacgggc
     3061 gccgggaggc tcggtcatct gtccgtaaat cagggcacaa cgagatttat cgggattgcc
     3121 cggctcgatc acgcccgatt cctgaaagtc cagccaaagg tcgttccctt cgcgggtgcg
     3181 ctctccgacg ccggcgaaaa ccgaaacgcc gccatgcttc atcgcgacat tgttgataag
     3241 ctcctggatg agcacggttt tgccaacgcc cgcgccgccg aagaggccga tcttgccgcc
     3301 cttgagatag ggctcgatca ggtcaatgac cttgatgccg gtttcgaaca tttccaggtt
     3361 ggtggattgg tcctcgagcg agggcgccga acggtgaatg gggtagcgtt tgtcggcttt
     3421 tactggcccg aagccatcga cgggatcgcc caggacgttg agcacgcggc cgagggtgcc
     3481 gcgtcccacc ggcactgaga ttggctcacc cagatcctga gctttcattc cgcggaccag
     3541 accctcggtt ggcttcatgg cgacgcatcg cacgcggtct tcgccaaggt gctgggccac
     3601 ttccgcaacg atgtccatcg gctcgggaat gtcgaatccc tcactccaga tgcgaacggc
     3661 attgtgaatt cgcggcaggt ggtgctcttc gaactgcacg tccacaaccg gaccgatgac
     3721 ttcgacgacg tgtccatttt ttgcgttatc gcttgccatt tcagctcccg taaattccgt
     3781 tgatgaaact cgtaaacgtt tcaatcctca cgccgctact tatgaacggc atcctcacga
     3841 catcgcggag gccgcgccac tgacgatttc gatcagctcc ttggtaattc ccgcctggcg
     3901 aatcttgttc atctgaaggg tgagcttgtc aataagctcg gaggcatttc gtgtagccgc
     3961 gtccatcgca gtcatgcggg cggcgtgttc cgcggcagcc gactcgagaa gaatgcggaa
     4021 aacctgcgat tcaaggtagc ggggcacgag gccgccaaaa atttcgtcca cgggctcctc
     4081 gtaaatgtaa tcgacaagcg attgtccgct tggatatcta tcatggggca gggaggcttc
     4141 ggcgtctctg cgcgttcctg tggatgtgtt cccagcttct tcggaaggcg atgcgagcac
     4201 atccggatcg ataggaagaa gcttttgggt gatgagtttt tgcaccatta cattcttgaa
     4261 ttcgttatag accgcataaa cagcatcaac ttcgcgactc gcatacaggt ccgcaattcg
     4321 gttggcaatt tcctttgcgg tggaaaactc caccttcgac gaaacattca gatattcgcc
     4381 gactgtgctc catcccctct ttcgcagcgt gtcccgaccc tttttcccga ttgcaatcaa
     4441 ctggtttttt ttggatgcat tctcgcgcaa gaacgcaatc gtgcggcgaa tcacattcga
     4501 attgaaagct ccggccaggc tgcgatcgcc tgtcattacc accagcagga tgcgctcctc
     4561 cggcctgcgc tcaaggaggg gatgcgcggt ggactccata cgcgccgccg ctgaacgcaa
     4621 cacgcggatg atttccttgg cataagggcg cgcggcaaaa acgccctctt gggcacggcg
     4681 aagttttgcg gccgagataa atttcatcgc acgcgtgatc tgctgggtat tcttaaccga
     4741 gcggatgcgg cgccgataat cgagaagtgt gggcattgat tgtcgtcggc ggatttagcg
     4801 ggccgcagtt gctgcctgat gagattcggg caagctggct cggaaccgct ctttgaaaga
     4861 gtccaaggcc ttggtggctt cggcgcgaat tgcgtcatcg agcgcctttt tgtctcgaat
     4921 cgcctgcagc acgccgggat gattcgtctc gacgaacgtg tacatctcga gctcgaatcg
     4981 gcggcattcg gagacgtcga tatcgtcaag ataacgattg gaactggcga agagcaacag
     5041 cacttgtttt tcgacaggca gcggctggta ttgatcctgc ttcaggattt ccatgagtct
     5101 ttggccgcgg gccaactgcg cctgggtagt tttgtcaagt tgttcggagc caaactgcgt
     5161 aaacgccgcc agctcgcggt attgggccat atctagccgg agcgttccgg ccacctgacg
     5221 cattgctttt atttgggcgt tgccgcctac gcggctgacg gaaatgcccg cgttgatagc
     5281 aggacgaata ccagcattaa aaagatccgc ttcgagataa atctggccgt ctgtaattga
     5341 aataacattt gtagggatgt aagccgagac gtcccctgcc tgtgtttcga tcacgggcag
     5401 ggccgtaagc gaacccgcac ccaatttgtc attcaatttt gccgcgcgct ccaggagacg
     5461 cgaatgcaaa aagaaaacgt cccctgggaa tgcctcgcga cccggcggcc tgcgaagcag
     5521 cagcgaaatc tcccggtagg attgcgcgtg tttggaaaga tcatcataga aacagatcgc
     5581 gtggcgcttg ttatcgcgga aaaactcgcc cattgctgca gcggcaaaag gcgcgatgta
     5641 ctgcatcgaa gccggctccg cagcggaggc cgcaacaacc atgctgtagt cgagagcgcc
     5701 gtgatcggaa agtgttttca cgacctgtgc gactgttgag cgcttctgcc caatcgcaca
     5761 gtagatgcaa atcatgtctc cgccgcgctg attcaagatt gtgtcgagaa taatcgcagt
     5821 cttgccggtc tggcgatcac cgataatcaa ttcacgctgg ccgcggccaa tgggaatcat
     5881 cgaatcgatg gccttgatgc ccgtttggag gggctctcga accggctgac gatcgaccac
     5941 gccaggc
//
LOCUS       DATNDT010000058         3934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3377950, whole genome shotgun sequence.
ACCESSION   DATNDT010000058 DATNDT010000000
VERSION     DATNDT010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3377950"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1216)
                     /locus_tag="VN788_01745"
     CDS             complement(<1..1216)
                     /locus_tag="VN788_01745"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU19285.1"
                     /translation="MRILLTSSASHLPPRGGSTRGNLAWLEYLSHSGHDCRVVGGVAP
                     AHSAEEFARVCRELQEEGLDPLAGESADLYRGIRIYSASKPEAQAALLREQIAVFHPD
                     WVLVSSEDLSHVLLKEAQRQAPGRIVYLAHTPQFFPFGPASWNADITAAEIVAHAAGV
                     IAIGNYMAEYIHSHCGRRPAVIHPPIYGEPPFPVRGRFGPGAITMINPSAVKGISIFL
                     ALADALPNERFAALPGWGTTERDRANLRARANITVLPNCRNIDEVFQKARILLMPSLW
                     LEGFGLAVVEAMLRGIPVIASNSGGLQEAKLGTGFIVPVRQIDSYELEFDDQGMPAAA
                     IPDQDLTPWRQALSALTGDRASYDREAALSRNAALEFVAGLRASHFESLLASLTVAPE
                     APATATRGNIGGL"
     gene            complement(1217..3385)
                     /locus_tag="VN788_01750"
     CDS             complement(1217..3385)
                     /locus_tag="VN788_01750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIC family phosphatase"
                     /protein_id="HXU19286.1"
                     /translation="MPAFPSGTHTFESETSGERRPSALVVCATFTVEPIAATLQFWAK
                     KLKLDYEVEFAPYGQIGQTLLDPAGLFATNQHGFNIILVRWEDWPGGAGAPDLVRIQE
                     NATDFFAVLRAAAARLKAPTVCCLCKPSKRFEKNFVQQLEKDAKAALQGVNGVYLLTN
                     SEIEERYPVPNYVDAQADILGHVPYTPPYFAALATAIARKIHTIVAPPYKVIALDCDN
                     TLWDGICGEDGPRGVRLNPAREAMHRFFLAQRDAGMLLCLCSKNNEADVFETFAAHPE
                     MPLRPEHFVSWRVNWGPKSSNLISLADELGLGLDSFIFVEDDPKECAEVEANCPEALT
                     IPLPVNSDDILELLNHIWAFDHTRLTEEDKRRSASYSQQAARGLFEKQARNLREFLDS
                     LQLEVRIGTMMPDQLPRVAQLTQRTNQMNFTAMRRSEAEIRALVEQGWECITVDASDR
                     FGDYGLVGVMMLRREDQTLVIDTFLLSCRALGRGIEHRMMAYAGRRAVELGCSHVDAP
                     FSRTPRNRPAEEFLESTGSTFRVSGTELFRYPVEYLCKLSYRPPEHNTAEEPNGSFVT
                     TPMDERKFRDYVGIARELRTPRQVLERARGERRVLASRGSDAPRTDLERQLAAIWEDL
                     LHVTPIGVHDNFFDLGGHSLMAVQLLSRLKREIGIDLSLEVVYSGELTIAEMAKAIEL
                     REFAGADTDEYAAILAEVESLSDEEVRALLEAEEQPDQSG"
     gene            3711..>3934
                     /locus_tag="VN788_01755"
     CDS             3711..>3934
                     /locus_tag="VN788_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HXU19287.1"
                     /translation="MIPSTETNCCWHTCRSDLRRKLAVILDAFSRKVVGWELDRTLAA
                     RLPIAALEKAIAERKPPSGVVHHSDRGVQYA"
BASE COUNT          834 a         1191 c         1103 g          806 t
ORIGIN      
        1 acagcccgcc gatattgccg cgcgtggccg ttgccggggc ctctggcgca accgtcagac
       61 tcgcgaggag actttcaaaa tgcgaggccc tcagacccgc cacaaattcc agagcggcat
      121 tgcgggagag tgccgcctca cgatcatatg aggcgcgatc gccggtcaac gcgctcagcg
      181 cctgacgcca tggcgtcagg tcctgatccg gaatcgccgc tgccggcata ccttgatcgt
      241 cgaactccag ttcgtagcta tcgatctggc gaactggcac gataaatcct gttcctaatt
      301 ttgcttcttg caagccaccg gaattgctgg cgatcacggg aatgccgcga agcatcgcct
      361 ccaccacagc cagcccgaat ccttccagcc aaagcgaagg catcaggaga atgcgagctt
      421 tttgaaaaac ctcatcgata ttccggcaat ttggcagcac cgtaatgttg gcgcgcgcgc
      481 gaaggttcgc gcgatcccgc tccgtcgtgc cccatcccgg cagcgcggca aatcgctcat
      541 tgggtaaagc gtccgccagc gcgagaaaaa ttgaaattcc ttttaccgcg cttggattga
      601 tcatcgtaat cgcgccgggt ccaaatcgtc ctctgacggg aaacggaggt tctccataga
      661 tcggcggatg aatcaccgcg ggtcttcgtc cgcagtgcga atggatgtac tccgccatgt
      721 aatttccgat ggcgataacg ccggccgcgt gagctacgat ctccgccgct gttatgtccg
      781 cgttccaact cgccggcccg aatggaaaaa actgcggcgt gtgcgccaga taaacaatgc
      841 ggcccggcgc ttggcgttgc gcctctttga gcagcacatg gctgaggtcc tcggacgaaa
      901 ccagaaccca gtccggatga aacaccgcaa tctgttcgcg gagcaatgcg gcttgcgctt
      961 ccggcttgct cgcggaataa attcgaatac cgcgataaag atcggcggac tctccggcaa
     1021 gcggatcgag gccttcttct tgcagttcgc ggcaaactcg cgcgaactcc tcggccgagt
     1081 gagccggcgc aaccccgccc acgacacggc aatcatgtcc gctatggctg aggtattcga
     1141 gccaagccag attgccgcga gtcgagcctc cgcgcggggg gagatgcgat gcgctggaag
     1201 tcagaagaat ccgcatctac ccgctttggt ccggctgttc ctccgcttcc aacagcgcgc
     1261 gaacttcttc atccgagaga ctctccactt ccgcgaggat cgccgcatat tcatcggtat
     1321 ccgcgccggc gaactcgcga agttcaatcg ctttcgccat ctccgcaatg gtgagttctc
     1381 ccgaatagac tacctccaac gaaagatcga tgccgatttc ccgtttcagc cgcgatagca
     1441 gttgtaccgc catcagcgag tgtcccccca gatcaaagaa gttgtcgtga acgccgattg
     1501 gagtcacatg caacaggtct tcccatatcg cggcaagttg ccgttcgaga tcggtccgag
     1561 gcgcgtcaga accacggctc gcgagaacgc ggcgttctcc tcgcgctcgt tccaacactt
     1621 gccgcggagt gcgaagctct ctcgcaatgc cgacgtaatc gcgaaatttt ctttcgtcca
     1681 tcggagtggt gacgaacgat ccattcggtt cttccgcggt attgtgctcc ggcgggcggt
     1741 aactcagttt gcaaagatat tcgacaggat aacggaacag ttccgttccg gaaacacgaa
     1801 aagtcgagcc agtgctttct aggaactcct cggctggtct gttccgagga gtgcgcgaaa
     1861 acggcgcgtc cacatgcgaa caaccgagtt cgacggcgcg ccgtccggca tacgccatca
     1921 tgcgatgttc gatccctcgc ccaagcgccc ggcaactcag caagaaagtg tcgatgacta
     1981 gcgtctggtc ctcgcgccgc aacatcatca cacccactag gccataatcg ccaaagcgat
     2041 cgctcgcatc gacggtaatg cactcccatc cttgctccac caacgcgcgg atctccgcct
     2101 cgcttcgccg catggccgta aagttcatct gatttgtgcg ctgggtaagt tgcgcgacgc
     2161 gcggaagttg atcgggcatc atcgtgccga ttcgtacttc cagttggagg ctgtccagaa
     2221 attcccgcag attgcgagcc tgtttttcga agagcccgcg tgcggcctgc tgcgagtacg
     2281 aagccgagcg gcgcttgtcc tcttcagtaa gacgcgtgtg atcgaacgcc caaatgtgat
     2341 taagcaattc gaggatatca tccgaattta cggggagcgg aatcgttagc gcctcggggc
     2401 aattcgcttc cacctccgcg cactctttgg gatcgtcctc aacgaagatg aagctgtcca
     2461 ggccgaggcc cagctcatcg gcgagcgaaa tcaggttcga cgatttcggc ccccaattga
     2521 cgcgccacga tacgaagtgc tccggccgaa gaggcatttc cggatgcgcc gcgaatgtct
     2581 cgaagacatc ggcttcattg tttttgctgc aaaggcagag cagcattcca gcgtcccgct
     2641 gcgcgagaaa gaagcgatgc atcgcttcgc gagccggatt gagacgaacg ccccgtggtc
     2701 catcttcgcc gcaaatgccg tcccaaaggg tgttgtcgca atcgagcgcg attaccttat
     2761 acggaggcgc aacgatcgtg tggatcttcc ttgcgatcgc ggtcgcaagc gccgcgaagt
     2821 acggcggagt atatgggaca tggcccaaga tgtcggcttg tgcgtccaca taattcggga
     2881 ctggatagcg ctcttcaatt tccgaattcg tgagaaggta aactccattc acgccttgaa
     2941 gagcagcttt ggcatccttc tccaattgct gcacgaaatt cttttcaaag cgcttggagg
     3001 gcttgcagag acagcagacc gtgggcgcct tcaggcgagc cgctgccgcg cgaagcactg
     3061 cgaaaaaatc cgtcgcattc tcttggatgc gtactaaatc tggcgcaccg gcgcctcccg
     3121 gccagtcctc ccagcggacc aatatgatat taaatccgtg ctgattcgtt gcgaaaagac
     3181 cggcgggatc gagcagcgtc tgccctatct ggccatacgg tgcgaactca acttcatagt
     3241 cgagcttcag ctttttcgcc cagaactgaa gcgtggcagc gattggctcg acagtgaaag
     3301 tggcgcagac cacaagcgcg cttggcctcc gctccccgga ggtttcagac tcgaaggtgt
     3361 gagttccgga tgggaaagcc ggcatcggtc aaccgagtgg attttcgcgc taaaacgcga
     3421 atccatccgc gattgtaaca aggagaggga cccggctcac ttggcagtgc gctaaatctg
     3481 cgtgtctggc ttacgcggcc tccagcagct cgcgtaccgt ccatctgcga cccggcggcc
     3541 attactggtg tcatactgag cgacttgtga aaccggcata agttgtaaaa gccgaaccac
     3601 actgcaacgg cggcccagtg gttctcccac gtatttgaaa aggtgtttgt cagccgcgtg
     3661 aaacaggccg tcaccaacac agccatacac atttagcgca ttaccatggg gtgatcccct
     3721 ccactgagac aaactgctgt tggcacacct gccgttctga cctgcgccgc aaattggcgg
     3781 tgatcctgga cgccttctca cgcaaggtag tgggctggga actggatcgg acgctggcgg
     3841 cgcgcctgcc gatcgcagcg ctggagaagg ccatcgcgga acggaagccg ccgtccggcg
     3901 ttgtgcatca ttccgaccgt ggggtccagt acgc
//
LOCUS       DATNDT010000059         9461 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_198474, whole genome shotgun sequence.
ACCESSION   DATNDT010000059 DATNDT010000000
VERSION     DATNDT010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9461)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9461)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9461
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_198474"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="VN788_01760"
     CDS             <1..573
                     /locus_tag="VN788_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015775543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HXU19288.1"
                     /translation="ELAEKGGPRRIAERYNRFAHGGTAEPLHATEKLSDGELTGQLFE
                     AVAEQHLNQPTFIYDFPTDISPLSKQRRDDPSLAERFELFIAGMEIANGFSELNDPAD
                     QERRFRAQVEKGGQDAPKEVDIDYIRALSHGLPPTAGEGIGIDRLVMLLTDSHAIREV
                     ILFPLLRAETPENPATASGEKGADKDGISK"
     gene            570..1796
                     /locus_tag="VN788_01765"
     CDS             570..1796
                     /locus_tag="VN788_01765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19289.1"
                     /translation="MNLEWLVALRYLRSPNRPAVLRLVTLLAILGVAAGVMTLVIALA
                     MNTGFRQAIRDRLLSVTAHVNLKPASPEGIRNYQEEIAKLGATPGVRSIEPAIYNTVL
                     LSIGGRARGVVLKGVDPELELRNGGALQHMVAGSNNFAPSADAIPVVLVGRILAEELK
                     VSAGDYVTLTSPQGNLTPFGMLPRARRFRVGGIFDSGFYDYDANWGFVTLASAQALAG
                     VGDVVSLLEVRVTNIDEARTVADELLRRAGPGFIATTWMDENRALFRALSLEKLVTAL
                     FIGLITFVAGLNILVVLTMTVADRARDIAVLMAMGTRRHQVRRIFILQGLVVCIGGTA
                     AGLLAGYGLSWIANRWRLIPLNPEVYAIPYVPFHANALDAIWIAAAALAICIAATIVP
                     AWSAARILPVEILRFE"
     gene            1867..2088
                     /locus_tag="VN788_01770"
     CDS             1867..2088
                     /locus_tag="VN788_01770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HXU19290.1"
                     /translation="MQRTTVYFSPKLYRALKVKAATTDRGFSELVNEAVQLALREDAL
                     DEEAYRRRQKEPSRAFATVLRELKSARLL"
     gene            2072..2332
                     /locus_tag="VN788_01775"
     CDS             2072..2332
                     /locus_tag="VN788_01775"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXU19291.1"
                     /translation="MPASYSLRIKRSAEKELRRIPKADLRRIVQRMERLATDPRPPGC
                     EKLFGDNVYRVRQGDYRILYAVDDGEGAIEVIKIGHGREVYR"
     gene            2716..3549
                     /locus_tag="VN788_01780"
     CDS             2716..3549
                     /locus_tag="VN788_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU19292.1"
                     /translation="MPVKANAGADNREADLGEASKSAPVLRVEGLRKTFCSGAQEITP
                     LNEIDFLAEQGEIIAIVGPSGTGKSTLLHLLAALDTPTSGTVYFAGNSLRTFSQMEMA
                     EYRNRAVGFVWQRHHLLPDFTAAENVAMPLLIRGQAWGQALRVATDWLGEVGLANRAR
                     QRAGELSGGEQQRVAIARSLANQPMLLLADEPTGDLDERSAEGIFELIERLHRSHHLT
                     SILATHNLALAGGADRMLVLEHGRLASKPDMSAMVGARSWRQASDTARPRAWERGVTG
                     V"
     gene            3542..5968
                     /locus_tag="VN788_01785"
     CDS             3542..5968
                     /locus_tag="VN788_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HXU19293.1"
                     /translation="MFERYTEKARRVIFFARYEASQYGSPYIETEHLLLGLLREDKAL
                     ANRFLRAHGSIESIRKEIESRITVRERISTSVEVPLSQECKRILNYAAEEAERLGHKH
                     VGTEHLLLGVLREEKSFGAEILLERGLRLSTVREEMVRTAGEKVPVNRPKEASLLAEF
                     SRDLTQAAMEGQLDPLVGRDGELERAVQILCRRTKNNPVLIGEPGVGKTAIVEGLAQR
                     IADGDVPPFLADKRILSLDLSLIVAGTKYRGQFEERLKTIMKELMESQNAIIFIDELH
                     TLVGAGSAEGSLDAANILKPALSRGEIQCIGATTPAEYRKSVEKDRSLERRFQAVKVS
                     APNEEDTIRVLQGVRDRYEKFHAVSYTEDAIQYAVYLSNRYIPDRFLPDKAIDLLDES
                     GARVKLRQAMLPDEVAEVQKRVKFITHRMETAIANHEFEKARFYSEEERKEKEHLRTL
                     REKLKIDDSSTGIVTREDIEEVVARWTGIAVMSIKEDEQQKLLRIEEELHRRVISQDK
                     AITSLARAIRRSRAGLKAPGRPVGCFLFLGPTGVGKTEVARRLGEFLFGSEKSLIRFD
                     MSEYMEKHSVSKLIGAPPGYIGYEEGGQLTERVRRTPYSVILLDEIEKAHPDVYNILL
                     QVFEDGQLTDGLGNTVDFRNTIIILTSNLGARQLQKQSGLGFQSADEVAAAKSQDDMV
                     MGEVKRVFNPEFLNRLDEVILFNSLTDQDLLLIIDLLVSQINETLVHRQVQIALTPEA
                     RQWILEKTCSDRNYGARPLRRALQKYVEDPLSEALIQGGLQRPATLEIFLSGDALSFR
                     PVVEATPVAH"
     gene            6045..8960
                     /gene="bamA"
                     /locus_tag="VN788_01790"
     CDS             6045..8960
                     /gene="bamA"
                     /locus_tag="VN788_01790"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HXU19294.1"
                     /translation="MTRSMAASSETVSRALEILAVVCFLSLAAVQSVWAQQAPESTGP
                     QYVITRIEFEGNRRVARDTLLTRIFSRPGDPYSADAVRRDFLALWNTQFFEDIWLEVE
                     DDPRQANGKIVIFHLKERPIIRRIEYKGNKSITESDILDAFKQKKVGLSVESQFDPTK
                     IKRAEVVIKELLAEHGRQFATVKPTYERIAASNAVKLVFNIDEGPKVKVGTITIVGNK
                     AFSSRRIIRTMRHDRPYGIPLGITYIPVMAKTFDRPKLDEDLEVGVRGLYQDHGYFKV
                     VVDVKDLKTVDLNRGGIPLPIPGIGSKHGKATNIIVSIEEGQQYRMGKLLFRSADPDQ
                     GLIFKPEVLARVFPLQPGDIFDADKIRKSLDNYRKLYGEYGYIDFTAEPEFDVHDDTK
                     VVNLTLQFDQQKQFFVRRIEFSGNTTTRDKVIRRELLLDEGQVFNNRLWELSILRLNQ
                     LGYFDTIKPENAELKRNVKAGTVDIRLKVKEKGKQSVSFSGGVSGLSGSFVGASYQTN
                     NFLGLGETLTLSAQVGNIQTNATFGFTEPYLFDRPISTGFTIFYSKFDYNTARQEGLL
                     LGQSVAIDPALQENYNTDSRGFTIFASYPLRKFSFARLGLSYGWSTTNITPFSQSATL
                     LFEFTKFTSLAGPSALNGIRQSQITPTITYDTRDSPMFPTRGKSFTYGASFAGGPLGG
                     NVNTVSNIFDMTYFHPVYHHRNVFAIHFKTSMISGFGGKEVPPNNRFYAGGENDVRGF
                     DFYTISPFVFIPFSTTTTITYVDPRRLNAQGQPTAVSLPVNVLEFIPTRPGGDLEAVS
                     NVEYRIPLVKSYVTMNFFHDLAVNGIMRRSQLQLDPSAIASLQQQYPNADFPCLPSTP
                     CLNVPRNLQIAHGTNFWPHASAGIELDFQIPIIQAPFRIFYAYNYSRFTQSIKPPLGA
                     FFVPPAERSSLQQLGVYNTQIVPDLQSFLNLTRSTQTVPPSLFEQKTTLRFAVSRTF"
     gene            9053..>9461
                     /locus_tag="VN788_01795"
     CDS             9053..>9461
                     /locus_tag="VN788_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19295.1"
                     /translation="MKAAGLATVLILLTVGLGGCASNSTPVAVTVTAASSSATVSGVT
                     VLVNSTVQFSASVTGGSNSSVFWQICKPTSTPQSTTIAPTDCSQGQGPASCAIPMVSK
                     PLTGFGTITSSGLYAAPGAPPQPNKFLVVATSCA"
BASE COUNT         2126 a         2655 c         2745 g         1935 t
ORIGIN      
        1 gagctggctg aaaagggtgg gcctcgacgc atcgccgagc gttataaccg tttcgcgcat
       61 ggcggcactg cggaacccct ccacgctacc gaaaaattat cggatggcga attgacgggg
      121 cagctttttg aagcggtcgc cgagcagcat ttgaatcagc ctaccttcat ctacgatttc
      181 cccaccgaca tttcgccgct atccaagcag aggcgggatg atccttcgct ggccgagcgg
      241 ttcgagctat tcatcgcagg catggagatc gccaatggat tttcggaatt gaacgatccg
      301 gccgatcagg aacgccgctt tcgagcgcag gtggaaaaag gcggccagga tgctccaaaa
      361 gaggtggata ttgactatat tcgcgcgctg agccacggtc tgccgccgac ggcgggtgaa
      421 gggattggga ttgaccgctt ggtgatgttg cttacggact cccacgcgat tcgcgaggtg
      481 atcctcttcc cgttgttgcg cgccgaaacg cccgagaatc cggcgacagc atccggcgaa
      541 aagggcgccg acaaagacgg aatctcgaaa tgaatctcga atggctggtg gcgctgcggt
      601 atctccgctc gccgaaccgg cctgcggtgc tgcggctggt gaccctgctc gcgattcttg
      661 gcgtcgcggc gggagtgatg acgctggtga tcgcgctggc gatgaacacg ggcttccggc
      721 aggccatccg cgatagattg ctaagcgtca ccgcccatgt gaatctgaaa cccgccagtc
      781 cggagggaat ccgcaactat caggaagaga tcgccaagtt aggggccacg ccgggcgttc
      841 gctcaatcga gccggccatt tacaacaccg tgcttttgtc gatcggcggc agggcgcgcg
      901 gagtggtgct caagggcgtg gacccggagt tggagctgcg caatggcggc gcgttgcaac
      961 acatggtggc cggctccaac aacttcgcgc cgagtgcaga tgccatcccg gtagtgctgg
     1021 tgggccggat tctcgccgag gaattgaaag tctccgcggg cgactacgtt acgctgacga
     1081 gtcctcaggg aaacctgacg ccctttggga tgctgccgcg cgcgcggcgc tttcgcgtgg
     1141 gaggaatctt cgattcaggt ttctatgatt acgatgcgaa ttggggtttc gtgaccctcg
     1201 cctccgcgca ggcgctggcg ggcgtgggag acgtggtgag cctgctcgaa gtgcgcgtta
     1261 caaacataga tgaggcgcga acggtagccg atgagttgct gcgccgggca ggccccggat
     1321 tcatcgccac aacctggatg gacgagaacc gcgcgctgtt tcgcgcgttg agcctggaga
     1381 agctggtcac agcgcttttt attggactaa ttacatttgt ggccgggctc aatatcctcg
     1441 ttgtgctgac gatgaccgtg gccgaccgcg cacgagacat tgccgtcctg atggccatgg
     1501 ggacgcggag gcaccaagtg cgccgaattt tcattttgca agggctggtt gtctgcattg
     1561 gcggaaccgc agcgggactc ttggccgggt acggactatc ctggatcgcc aatcgatgga
     1621 gattgatccc tctaaatcca gaggtgtatg ccatacccta cgttccgttc cacgcaaatg
     1681 cgctggacgc gatttggata gcggcggcag ctttggcaat ttgtattgcc gccacgattg
     1741 ttccggcatg gtcggcagct cggatcctgc cggttgagat tctccgcttc gaatagcccc
     1801 cgtagcacct tttgacacca tagcatcata atgtcataat tgtttccaga ggtccgatat
     1861 caagccatgc aacgcaccac tgtctatttt tcacccaaac tctaccgggc tttgaaggtt
     1921 aaagcggcaa cgaccgatcg gggtttctcc gaactggtga acgaagccgt gcagctcgct
     1981 ctcagggaag acgccctgga cgaggaagcg taccgacgca ggcagaagga gccgagccgc
     2041 gcgttcgcga cggtcctgcg tgaactgaaa agtgcccgcc tcttatagcc tgcgaatcaa
     2101 gcgcagcgcc gagaaggagc tccgtcgtat cccaaaggct gatttgcgcc ggattgtgca
     2161 acgaatggag cgcctggcta cagaccctcg gcccccgggt tgcgagaagc tatttgggga
     2221 taatgtatat cgcgtcagac aaggggacta ccgcattctc tacgcggttg acgacggaga
     2281 aggcgcgatc gaagtcataa aaatcggtca tggtcgggag gtctatcgct gacgcttcga
     2341 ttacggtgac caggcgcgct tcgcgaagaa tcgccgggct agcctccgcc gaaaggcata
     2401 tcgccgatct gacatagggc gcggtagcgg aaacagcccg gcacagggtt tccggcgcga
     2461 aaaagactgg caaattatcg ctcgaatttg gccttcgatt cggttctatg acggtgtgcg
     2521 tggtaaaatg aaaaaggtgg cagtgagacc tgaccaaaaa ggtggcaaac cgccggtcgg
     2581 ctttagggaa ataatcggga caagtgagaa acgaattagg ccggactaca gcgcgggaaa
     2641 ggccgcgagt actggaaaac aaggacatac cgaggaacgt gttcggcatc taaactgtga
     2701 taggaataag cgtcgatgcc agtgaaggcg aatgcgggcg cagacaaccg ggaggcagat
     2761 ctcggagagg cctccaaatc ggcccccgta cttcgagtgg aagggcttcg aaagaccttc
     2821 tgctccggcg cgcaagaaat aacacccctc aacgagattg attttttggc tgagcagggc
     2881 gaaataatcg ccatagtggg gccctccggc accggaaaaa gcacgctgtt gcatctgctg
     2941 gcggcgctgg acactccaac aagcggtaca gtatacttcg ccggaaattc gcttcgcacg
     3001 ttttcgcaga tggagatggc ggaataccgc aatcgtgcgg tgggttttgt gtggcagcgg
     3061 catcacctgc tgcccgactt cacggcggcg gaaaatgtgg ccatgccctt gctgattcgg
     3121 gggcaggcgt gggggcaagc actgagagtg gcgacagact ggttgggtga agtcgggctg
     3181 gcaaaccggg ccaggcagcg ggcgggcgaa ctttctggcg gtgaacaaca gcgagtggcg
     3241 atcgcacgtt cccttgcgaa tcagcctatg ctgctcctag cggacgagcc aaccggcgat
     3301 cttgacgaac gcagcgccga aggcattttt gaattgatcg agcgtctcca tcggtcccac
     3361 catttgactt ccatcctggc gacgcacaat ttagcgctgg ctggaggggc cgatcgaatg
     3421 cttgttctgg aacatgggcg gctggcgtca aaaccagaca tgtccgcgat ggttggtgcg
     3481 cgcagttggc gccaagcatc tgatacagcg aggccacgag cctgggagcg tggggtgacc
     3541 ggtgtttgaa cgctacacgg agaaggcgcg gcgggtaatt ttcttcgcgc gatacgaagc
     3601 gagccagtat ggaagcccgt acatcgagac cgagcacctg ttattgggac tcttgcggga
     3661 agataaggcg ctggcgaatc gctttctgcg tgcccatggc tcgatcgaat ccatccgcaa
     3721 ggaaatcgaa tcgcgaatca ccgttcgcga gcgcatttcc acttccgtgg aagttccgct
     3781 cagccaggag tgcaagcgca ttctcaacta cgccgccgag gaagcagagc ggctcggcca
     3841 taagcatgtg ggaacggaac acctccttct gggagtcttg cgagaggaaa agtcatttgg
     3901 cgccgaaatc ctgctggaac ggggattgcg gctttccacc gtgcgcgagg aaatggtgcg
     3961 cactgccgga gagaaggtgc ccgtgaaccg tccaaaagag gcttccttgc tggctgaatt
     4021 cagccgcgat ttgacgcaag cggctatgga gggtcaactc gatcccctgg tgggccgtga
     4081 cggcgagctc gaacgcgccg tacagatcct ttgccggcgc accaagaaca atccggtgct
     4141 gatcggcgag cctggcgtgg gcaaaacggc aatcgtggaa ggcctggcgc agcggatcgc
     4201 cgatggcgat gtgccgccat ttttggccga caagcgaatc ctgtctctgg acctttcgct
     4261 gatcgtggcg ggcaccaagt atcggggcca gtttgaagag cggctgaaaa ccatcatgaa
     4321 agaactgatg gaaagccaaa atgcgatcat cttcatcgac gaattgcaca ctctggtggg
     4381 agctggctcg gcggaaggtt cgctcgatgc cgccaacatt ttgaagcccg ctctgagccg
     4441 cggcgaaatt caatgcatcg gcgcaaccac tccggccgag tatcgcaagt cagttgaaaa
     4501 ggaccgttcg ctcgagcggc gattccaggc cgtgaaagtt tccgcgccga acgaagagga
     4561 taccattcgc gtgctgcaag gcgtgcgaga tcgctacgag aaatttcacg ccgtcagcta
     4621 taccgaggat gcgattcagt acgcggtcta tctctcgaat cgttacatcc cggatcgctt
     4681 cctgccagac aaggcgattg atctgctcga cgagtccggc gcgcgcgtga agttgcggca
     4741 agccatgctg ccggacgaag tagccgaagt gcaaaagcgc gtgaagttca tcacacacag
     4801 gatggaaacc gcgatcgcga accatgagtt cgaaaaggcg cgattttatt cagaggaaga
     4861 gcgcaaggaa aaagagcacc tgagaacgtt gcgcgaaaaa ctcaagatcg acgattcctc
     4921 caccggaatt gtgacgcgtg aagatatcga ggaagttgtg gcacgctgga ccggcatcgc
     4981 ggtgatgtcg atcaaagaag acgagcagca gaaactgctt cgaatcgaag aagaattgca
     5041 ccggagggtg atcagccagg ataaggccat cacttccctg gcgcgtgcca ttcggcgcag
     5101 ccgcgcggga ctgaaggcgc cgggcaggcc ggtcggttgc ttcctgttcc tcggccctac
     5161 gggcgtcggg aaaaccgaag tggcgcggcg gctgggtgag ttccttttcg gcagcgagaa
     5221 atcgctgatc cggttcgata tgtccgaata catggagaag cattccgttt cgaagttgat
     5281 cggcgcgccc ccgggatata tcggctatga agaaggcggg cagctgacgg agcgcgtccg
     5341 ccgcacgccc tattcggtga tcctgctcga tgaaatcgag aaggcccatc cggacgtgta
     5401 caacatcctg cttcaggttt tcgaagacgg ccagttgacg gacggcctcg gcaataccgt
     5461 tgacttccgc aacacgatca tcattctcac gtccaatctg ggcgcccggc aattgcagaa
     5521 gcagtcgggg cttggctttc agtcggcgga cgaagtcgcc gcggcgaaga gccaggacga
     5581 tatggtgatg ggcgaagtga agcgagtctt caatcctgaa ttcctgaacc ggctggacga
     5641 agtgattttg ttcaattcgc ttacggatca ggacctgttg ctcatcatcg atctgttagt
     5701 gagccagatc aacgaaacgc tggtacaccg gcaggtgcag attgcgctga cgcccgaagc
     5761 ccgccaatgg attcttgaga agacctgcag cgacaggaat tacggcgcgc ggccgctccg
     5821 gcgcgccttg cagaagtacg tcgaagatcc actgtcggaa gctctcatac agggcggact
     5881 ccaaagaccc gcgacgctcg aaattttcct ctctggcgac gctctttcct tccgtcccgt
     5941 tgtggaagca accccagtgg cccactaaga atcctacacc gcgcgagccg gattaatacg
     6001 gatccggcgg gggagcaaaa aaaattgggc ggagtactgc ggccttgacc agaagcatgg
     6061 ccgccagcag cgaaactgtt tcgcgcgcct tggaaattct ggccgttgtg tgtttcctct
     6121 ctctcgccgc agtgcaatcg gtttgggctc aacaggcgcc ggaatccacc gggccgcaat
     6181 acgtcatcac gcggattgaa tttgaaggaa accgccgcgt cgcgcgcgat accctgctca
     6241 cgcgcatttt ttcgcgaccg ggcgatccct acagcgccga tgctgttagg cgtgattttc
     6301 tggcgctctg gaatacgcaa ttcttcgagg atatttggct cgaggttgag gacgatccgc
     6361 gccaagccaa cgggaaaata gtaattttcc acctgaaaga gcggccgatc atccgccgca
     6421 tcgagtacaa aggcaacaag tcgatcacgg aatcggatat tctggacgcc tttaagcaaa
     6481 agaaagttgg gctgagcgtc gagagccaat tcgatcccac aaaaattaag cgggccgaag
     6541 tggtgatcaa ggaattgctc gccgagcacg ggcgccagtt tgccactgtg aagccaactt
     6601 acgagcgtat tgcggccagc aacgccgtta aattggtctt caacatcgat gaggggccca
     6661 aggtcaaggt cggcacgatc actattgttg ggaacaaggc tttttccagt cgcagaatca
     6721 ttcgaaccat gcgccacgac cgtccttacg gaatcccact tggtatcact tacattcctg
     6781 taatggccaa gacctttgac cgccccaaat tggacgagga tcttgaagtc ggcgtccgag
     6841 gattgtatca ggatcacgga tatttcaaag ttgtcgtgga tgtcaaagat ttgaaaacgg
     6901 tggacctgaa tcgcggcgga atccccctgc ctataccggg aatcggttcg aagcatggca
     6961 aggccaccaa catcatcgtt tcgatcgagg aagggcagca atatcggatg ggcaagctgc
     7021 tattcaggag cgcggatccc gaccagggat tgatcttcaa gcccgaagtg ttggctcgcg
     7081 tgttccctct acagcccggg gatatttttg acgccgacaa aatacgcaaa tcgctcgaca
     7141 actacaggaa gttgtacggc gaatacggtt acatcgattt caccgccgag ccggaattcg
     7201 atgttcacga cgacaccaag gtcgtgaacc ttacgctcca gtttgaccag caaaagcaat
     7261 tcttcgtccg ccgcatcgaa ttttcgggca acaccaccac gcgcgataaa gtaattcgcc
     7321 gcgaactttt gctcgacgaa ggacaagtgt tcaataaccg tctctgggaa ctaagtatcc
     7381 tgcgccttaa tcagctcggc tatttcgaca ccatcaagcc cgaaaacgcc gaattgaagc
     7441 gcaacgtgaa ggcaggcacc gtcgatatca ggctgaaggt gaaggaaaaa ggcaagcaat
     7501 ccgtcagttt ctccggtggc gtcagcggcc tttccggtag tttcgtcggt gcctcgtatc
     7561 agaccaacaa tttcctggga ctcggcgaaa cactcacact ttcggcgcag gtaggcaaca
     7621 ttcagacaaa tgccaccttc ggtttcaccg agccgtattt attcgaccgc ccgatctcga
     7681 ctggattcac catcttttac tccaagttcg attacaacac ggcgcgccaa gagggcctgc
     7741 ttctcggtca atcggtcgct atcgatcccg ccctccagga aaattacaac acggactcgc
     7801 gcggtttcac catcttcgcc agctatccgc tgcgcaaatt ttcctttgcc cgccttggct
     7861 tgagctacgg atggtcaaca accaacatta cgccgttcag ccaatcggcc acgctgctct
     7921 tcgaattcac taagttcacc agcctggcag gaccttcggc gctgaatggc atcaggcaaa
     7981 gccagatcac tcccacaatt acctacgaca ccagggacag ccccatgttc cccacgcgtg
     8041 gaaaaagctt cacctacgga gcatctttcg cgggcgggcc gctcggcgga aacgttaata
     8101 cggtcagcaa tatttttgac atgacgtatt ttcatcccgt ttatcaccac cgcaacgtat
     8161 tcgccatcca tttcaaaacc agcatgatct ccggctttgg aggcaaggaa gttccaccga
     8221 acaatcgctt ttacgccggc ggtgaaaacg acgtccgcgg cttcgacttt tacaccattt
     8281 ctccttttgt ctttatcccg ttctccacga cgacaacgat cacgtacgtc gatccgcggc
     8341 gactgaacgc ccaaggtcag ccgacagcgg tttcattgcc ggtcaacgtg ctggaattta
     8401 ttcccacgcg gccgggtggt gacctcgaag ccgtatccaa tgtcgaatac cggattccgc
     8461 tcgttaaatc ctatgtcacc atgaactttt tccacgatct tgcggtcaat ggcatcatgc
     8521 gaaggtcgca gctccaactc gatccctccg caatcgcttc gttgcaacag caatatccca
     8581 acgccgattt tccatgcctg cccagtacgc cctgcctgaa tgtgccgcgc aatctccaaa
     8641 tcgctcacgg aacgaatttc tggccgcacg cctccgcggg tatcgagttg gattttcaga
     8701 ttccgattat tcaggcgcct ttccgcattt tttatgcgta taattactcc cgttttactc
     8761 agtccatcaa gccgccgctc ggtgccttct tcgttcctcc ggctgagcgc agcagtttgc
     8821 agcagttggg cgtctataac acacagatcg tgccggacct tcaaagcttc ctgaatctaa
     8881 cccggtccac gcaaactgtc cccccgagct tattcgagca aaaaacgacg ttgcgattcg
     8941 cagtgagccg gacattctga tgaagcttcc gacaccgctc gagacgaccg ggcttcggct
     9001 gccacacagg gaccaaatac atttcaaccg acgaatgccg ggaggcaagg acatgaaagc
     9061 cgcggggctg gcgacggtgc tgatactcct tactgtgggc ctgggcggct gtgcgtcgaa
     9121 ctcgacgccg gtggccgtta ccgtcacagc cgcaagctcc agcgcgacgg tcagcggtgt
     9181 aacggtcctg gtgaatagca cggtgcaatt ctcggccagc gtcaccggag gttccaacag
     9241 cagcgttttc tggcaaatat gcaagcccac aagcacgccg caatcgacaa ccatcgcgcc
     9301 gacggattgc agccagggcc aaggtccggc gtcatgtgcc atacccatgg tttccaagcc
     9361 cctcactggc ttcggcacca tcacttcgag tgggctctac gcggctcctg gcgctccgcc
     9421 gcagccaaat aaattcctcg ttgtggcgac cagttgcgcg a
//
LOCUS       DATNDT010000060         6470 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2767986, whole genome shotgun sequence.
ACCESSION   DATNDT010000060 DATNDT010000000
VERSION     DATNDT010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6470
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2767986"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VN788_01800"
     CDS             <1..536
                     /locus_tag="VN788_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19296.1"
                     /translation="SNHDQNASQHQAPNGVEIGSGTFQGQAAVTVSATSGVSVQLDKN
                     GNVTSITVTTVTAYFGTGNNAGQFLGAESTSTTHVPLSGADPVSTTTPLNFGQATRVM
                     GADRMALGPQTASGDILTHFPGAVASDVQAHPAAYLIKVGEAASLAIPVGEAYEGVKA
                     VVDVAAAAADFVYSLAH"
     gene            545..898
                     /locus_tag="VN788_01805"
     CDS             545..898
                     /locus_tag="VN788_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19297.1"
                     /translation="MERVNISRTTMVVAMAICLAEAAKFPHHDPGTGATVVMGILLLR
                     RVLTVFQPAAVVISGCVLTAIIILGDQGKVDIERHLWFWFPVIIVAFVIFGFWERIER
                     LWKHANNVPSKSGMV"
     gene            complement(1039..2100)
                     /locus_tag="VN788_01810"
     CDS             complement(1039..2100)
                     /locus_tag="VN788_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXU19298.1"
                     /translation="MPSMSGPLQTAVPHEFEAGPFGKLEITGILSGIGLTEGNHIFGD
                     PSTHWDVSNAQVFIQKSTGWWQFYLQGGAYNLPALGAPFLSTGDTVKNIYGAFPQGYF
                     KVVKGGFSAEIGALPTLIGAEYTFTFENMNIERGLLWNQENAVNRGIQLNETYKKLSA
                     SLSWNDGFYSNRYTWLWGSVSYAFNSANTLSFVAGGNAGSYAKNTLVTPVFLNNEEIY
                     NVIYTYTKGAWVVQPYFQYTNVPTNTRIGIFQGAATRGGAVLLTYNCKNGVSLAVRPE
                     YISTTGSVASGAINLLYGPGSGAFGLTLTPTYHKDAFFIRGEFSIVHATSSTPGDAFG
                     LAGLNVNQPRGVLETGFLF"
     gene            2745..3914
                     /locus_tag="VN788_01815"
     CDS             2745..3914
                     /locus_tag="VN788_01815"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HXU19299.1"
                     /translation="MTHSKKIGILTGGGDVPGLNPVIKSVVYGGGEIGRRVIGIRKGW
                     EGLVQMDGPDDPTYIRELDRDNTRTIDRSGGTVLHTSRTNPIHIPESKLPKSVPASEL
                     QKLKFDGKNYDLTPVVLRNLERLGIGCLVAIGGDDTLSFASILSAAGFPVIGIPKTMD
                     NDVQGTEYCIGFSTAVTRAKQSITRQRTTVGSHERIGIFRIFGRDAGFSALYTSYVTS
                     TRCLIPEHPFNLERVCSLLMEDKKNNPSNYALVVVSEGAIWTQGEVKEYGEADAYGHR
                     KKADIGTALAEEVRKRTGQETMVSDLTYDLRSGEPDSIDQIVATSFANIAVELIREGI
                     SGRMIGIQSGHYAHVPLPDSALGARKVNVRALYNTERYRPNYASKLGSPLFFSGS"
     gene            4074..4301
                     /gene="rpmE"
                     /locus_tag="VN788_01820"
     CDS             4074..4301
                     /gene="rpmE"
                     /locus_tag="VN788_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014697411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HXU19300.1"
                     /translation="MKEGIHPDYHPVTVHCACGSTFETRSTIKGQMIRVEICSSCHPF
                     FTGKQKLVDTAGRVERFQKKYADKAASIAKK"
     gene            4475..6064
                     /locus_tag="VN788_01825"
     CDS             4475..6064
                     /locus_tag="VN788_01825"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HXU19301.1"
                     /translation="MATRLSPVPDYPQGSAGSPIETSSPRVTLRNSFDHPFDSAMAAA
                     RTCYSPRLIGPDEITEKQRVTIGAGTYFGGHHTVYQHAHFEFGLENISRQFVWSYLHA
                     HPFYNSEQQSQRYVRLDLPQAYVPSEAPGFGAAERAIYEKAVVRAWEHYRELTSVLEP
                     TTREILNDIWHVGAMSHARRVQKVERQSEKRAIELARYVLPVAAFTTMVHTISGIVLY
                     RLWRMCAASDTPTEARQVVGEMVARVRESDPEFLDRFGNEPLDVATEFEAAPRDGAEF
                     AREFDARLAGATSRLIDYSPHAIESMTEAYRAVMGLRAEECSDAEALDRMLNPERNPY
                     RRETLNVGVHAPTMRPLDHAHFTFAKKISHTADSQDQRHRMVPGSRPLLTLADTREPD
                     YITPMLIRENPRALESYEQAMHEAWAAKNELLDRGVSREFALYLLPNAKAVRFVESGS
                     LLHLAHKWTMRTCFNAQEEIYQASIQEVEQVRRVFPELGRYIGPPCFLRAGISTPICT
                     EGSHFCGVKVWLDFPNVERRI"
     gene            complement(6210..>6470)
                     /locus_tag="VN788_01830"
     CDS             complement(6210..>6470)
                     /locus_tag="VN788_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19302.1"
                     /translation="RGEAYLAAHRGSEAAGEFQKILDHRGIVGDAVIGALAHLQIGRA
                     YALQGDTAKARAAYTDFLTLWKDADADIPILKQAKAEYAKLN"
BASE COUNT         1428 a         1829 c         1815 g         1398 t
ORIGIN      
        1 agtcgaacca cgaccagaac gcttctcagc accaagcgcc gaacggagtc gagattggaa
       61 gtggaacatt ccaaggtcaa gcagcggtga cagtaagtgc gacttctggc gtatcggtac
      121 aactcgacaa gaatgggaac gttacatcca ttacagtcac taccgttacg gcgtatttcg
      181 gtacaggcaa caacgcgggg caatttcttg gagcggagtc aacctctact acccatgttc
      241 ctctttcggg tgccgatccc gtctcgacaa ctactcccct gaatttcggc caggccacga
      301 gagtcatggg cgctgacaga atggcgctcg gcccacaaac ggcgtccgga gacatcctta
      361 ctcactttcc tggcgcggtg gccagcgatg tccaagcgca ccccgcggcg tacctcatta
      421 aagtcgggga ggcagcgagc ctcgcaatac cggttggtga agcctatgag ggcgtcaagg
      481 cagttgttga cgtggctgca gcggcggctg atttcgtcta tagtttggca cactgaagac
      541 agtcatggag agagtcaata tttcaagaac gacgatggtt gtggccatgg caatctgcct
      601 tgccgaggcc gcgaagtttc ctcaccacga tcccggtacc ggggcaaccg tcgtgatggg
      661 gattctgctc ttacgccgtg tgctaaccgt atttcagccc gccgctgtcg tgatttcagg
      721 gtgcgttttg acggcgatca tcatcctcgg ggatcaagga aaggtagaca tcgagcggca
      781 cctgtggttt tggtttcccg tcattatcgt tgcgttcgtg atttttggat tttgggagag
      841 aatcgaaaga ctttggaaac acgcgaacaa cgtgccctcg aagtctggga tggtctagtt
      901 cgatgacaag gactcttcgg ccgctggctc tccgccgact ggtccgcggt ccccgctccc
      961 gtgccctacg ccaacctgac caatcctcag acgctcaatc tctacgccat ggtcagcgac
     1021 aatcccgaaa tcttaatttc aaaacaagaa tcccgtctcc agaacaccgc gcggctggtt
     1081 cacattcaag ccagcgagac cgaaggcgtc gcccggcgtc gagctggttg cgtgaacgat
     1141 agaaaactct ccacgaatga aaaatgcgtc cttgtggtag gtcggggtca gcgtcaatcc
     1201 aaacgcgccg ctgcccgggc catagagcaa attgatggct ccgctcgcga cgcttcccgt
     1261 agtcgagatg tactccggcc gcaccgccag ggacactcca ttcttgcaat tgtaagtgag
     1321 caggacagcg cctccccgcg tagccgcacc ctgaaatatt ccaatcctcg tattggtcgg
     1381 aacgttggtg tactggaagt atggctgcac tacccaagcg cccttggtgt aggtgtaaat
     1441 cacgttgtag atttcttcgt tgtttagaaa cacgggcgtc acaagagtat tcttcgcata
     1501 cgatcccgcg ttacccccgg caacgaacga tagagtattt gcagagttaa acgcatagct
     1561 tacggagccc caaagccatg tatagcgatt ggagtaaaaa ccatcgttcc agctgagcga
     1621 ggcgctgagt ttcttgtagg tttcattgag ctggataccg cgattgacag cgttctcctg
     1681 gttccaaaga agcccgcgct cgatattcat gttttcaaat gtgaaggtat actcggcgcc
     1741 aatcagagtt ggcagcgcgc cgatctccgc tgagaacccg cccttaacca ccttaaagta
     1801 accttgcgga aacgcgccgt agatgttttt cacagtatcg ccggtcgaga ggaagggcgc
     1861 tcccagtgcg ggcaaattgt aggcgcctcc ttgaagatag aattgccacc agccggtact
     1921 cttttgaatg aaaacctgtg cgttgctgac atcccaatgc gtggatggat ccccgaaaat
     1981 atgattccct tccgtcaaac ctatgccgct caggatgccc gtaatctcta atttcccaaa
     2041 gggacccgct tcgaactcat gcggtacggc agtttggaga ggccccgaca ttgaaggcat
     2101 aggcaggggc atcggcgcgg ccgcgttttg cgccggagtg ggtgcctgcg gcgggttcgc
     2161 aggcgttgga gcgccttggt cctgcgctac cgccggactc gatgtgccac ccagcgcagt
     2221 ggaactcgac gcagccggag agttcgagtc aacattctgc tgtccgaaag aaggaaatgc
     2281 ggctaacgct ataaatgaaa gagcaaacaa gcacgtcctg ggtacgcgca aaactagtct
     2341 cctttggaac tcgagaccga ccgctaagtt cttcatcgtg cgattcatgt ggttcctctc
     2401 cctcccttcc gcgcccacat tcgcctcttc gtgagttcgc gggcctatcg acgctgtccc
     2461 tgctcagaaa ttggtgggga tacgctacag gcgctcagcg gaatggtcca actcatagaa
     2521 ttgatgcgag gcattcagga ataaaatcct tacgtattct ttattttctg gcatgacgcc
     2581 ggttcgagac tccgaagcgc tttagcccgt aaggaagcga tttgcttttc ctctgttttc
     2641 gaattaggaa attacggaat tgcgttgcgc atggacgcgg aagatgagca aaattagcac
     2701 gcgagaaatt gcggcggccg caggccgagc ggcggaggac tgacgtgacc cacagcaaaa
     2761 aaattggaat ccttaccggc ggtggcgacg ttcccggcct gaaccccgtg atcaagtccg
     2821 tcgtctatgg cggcggagaa atcgggcgcc gggtaatagg aatccgcaag ggctgggagg
     2881 gcctcgtcca gatggatggc ccggacgacc ccacctacat tcgcgaactt gatcgcgata
     2941 atacgcgcac catcgaccgt agcggcggca ctgtgctgca cacttcgcgc accaatccga
     3001 ttcacatccc cgaatccaag ctgccgaaaa gcgttcccgc aagcgaattg caaaaactca
     3061 aattcgatgg aaaaaattac gatctcaccc cggtcgtcct tcgcaatctc gaacggctgg
     3121 gtatcgggtg tcttgtcgcc atcggcggcg acgatacgct aagcttcgcc tcgattctct
     3181 ccgctgctgg atttccggtg attggaattc ctaagaccat ggataacgat gttcagggca
     3241 cggagtactg catcggattt tcaaccgccg tcacccgagc caagcaatcc attacccgcc
     3301 agcgcaccac cgtcggctcg cacgaacgca ttggaatctt tcgaattttc ggccgcgatg
     3361 ctggcttcag cgccctttac acttcgtatg tcacctccac gcgctgcctg atccccgagc
     3421 accccttcaa cctcgagcga gtatgcagcc tgctgatgga agacaaaaaa aataatccca
     3481 gcaattacgc gcttgtggtc gtttccgaag gcgccatctg gactcagggc gaagtgaagg
     3541 agtatggcga ggccgacgcc tatggccacc gcaaaaaggc cgatatcggc accgccctcg
     3601 cggaagaagt tcgcaaacga accggacagg aaactatggt cagcgatcta acctacgatc
     3661 tccgtagcgg cgagcccgat tctattgacc agatagttgc tactagcttt gctaacattg
     3721 ccgttgaact catccgcgaa ggcatcagcg gccgcatgat cggcatacaa agcgggcact
     3781 acgcacacgt cccgctgccg gattcggcgc ttggcgcgcg caaagtgaat gtacgcgctc
     3841 tctataacac cgagcgctac cgtccaaatt acgcttcgaa actcggctcg cctctgtttt
     3901 ttagcggctc gtgatcctga cggcgagcgc ctcgattctt ttgcggcttg cgagcccatt
     3961 aggtgtgaac gcgactctcc ggtataataa agattccccg ccagcctagc gacgcgaacg
     4021 gtgctgcatt gctcgacttt cgcgcagaac ggccggcaga caggagtttt tccatgaaag
     4081 aaggaattca tcccgattac catcccgtga cggtgcattg cgcttgcggc agcacctttg
     4141 aaacgcgctc gacgatcaag ggccagatga ttcgcgtgga aatttgctcg agctgccacc
     4201 cattcttcac cgggaagcag aaattggtgg acaccgcggg gcgcgtggag cgcttccaga
     4261 aaaaatatgc ggacaaagct gcgtcgatcg caaaaaagta gggctgagcg ccctttccgg
     4321 gcgatttgcg accgtttggg acgctttatt gtttctagtt agaagaatag gacgctacaa
     4381 actagaagca tttaatgctg caaactcgaa ggatttttcg cggtttctgt tctccgcccg
     4441 gtgaatttgg cgccgggtgt acaatgcggt gaagatggcc acgcgattgt ctcccgtacc
     4501 agattatccg caaggatccg cgggatcgcc gattgagacg tcgtctccgc gcgtaacgct
     4561 gcgcaattct ttcgaccatc ccttcgattc ggcgatggct gcggcgcgca cgtgctattc
     4621 cccgcggctg atcggtcccg atgaaatcac ggaaaagcag cgcgtgacca ttggcgcggg
     4681 cacgtatttc ggcgggcatc ataccgttta tcagcacgcg catttcgaat ttgggcttga
     4741 gaatatcagc cggcaattcg tgtggtccta cctccacgcg cacccgtttt acaactccga
     4801 acagcagagc cagcggtacg tgcgactcga tcttccgcaa gcgtacgttc ccagtgaagc
     4861 tcctggattc ggcgcggccg aacgcgcgat ttatgaaaag gcggtagtgc gggcgtggga
     4921 acattatcgc gaactgacaa gtgtcctcga gccgacgacg cgcgaaattc tcaacgacat
     4981 ttggcatgta ggcgcgatgt cgcatgcgcg gcgcgtacaa aaagtggagc ggcaatcgga
     5041 aaagcgcgcg attgagctgg cgcgttatgt gttgccggtg gcggcgttca ccacgatggt
     5101 tcacacgatt tccggaattg tgctttaccg attgtggcgg atgtgcgcgg cgagcgacac
     5161 gcccacggaa gcacggcaag tggtcggcga aatggtggcg cgcgttcgcg agagcgatcc
     5221 tgaattttta gaccggttcg gcaacgaacc gctggatgtt gccaccgaat tcgaagctgc
     5281 gccgcgcgat ggcgcagaat tcgcgcgaga atttgacgcg cgcctcgcgg gagctacttc
     5341 gcggctgatc gattattcgc cgcatgccat cgaatccatg accgaagcgt accgcgcggt
     5401 gatgggcctg cgtgcggagg aatgttccga cgcggaagcg ttggaccgga tgctgaatcc
     5461 ggagcgaaat ccctatcgac gcgaaacttt gaacgtgggc gtgcacgcgc cgacaatgcg
     5521 tccgctggat cacgcgcatt tcacgtttgc gaaaaaaatc agtcacactg cggatagtca
     5581 agaccaacgg catcgaatgg taccgggatc gcgtccgctg ctgaccttgg cggacacgcg
     5641 cgagccggac tacattacgc cgatgctgat tcgcgaaaat ccgcgcgcgc tcgaaagtta
     5701 cgagcaagcc atgcacgaag cgtgggcggc aaagaacgaa ctgctggacc gcggcgtttc
     5761 tcgtgaattc gcgctttacc tgctgcccaa tgcaaaggcc gttcgattcg ttgaatcggg
     5821 ctcgctgctg catctggccc ataaatggac gatgcgaacg tgctttaacg cgcaggaaga
     5881 gatttatcag gcgtccatcc aggaagtgga acaagtgcga agagtttttc ccgaactcgg
     5941 gcggtacatt ggaccgccgt gctttctccg ggcagggatt tccactccca tttgcaccga
     6001 aggctcgcac ttttgcggag tgaaagtgtg gctcgatttc cccaacgtag agcgccggat
     6061 ttagctggat ctctctcgat tcgtggtgcc ctgataaacg tccaatctgg aaaagcgaag
     6121 tactatttcg catctccggt aacccgacta accgtccact taacatcctg agaggctcgc
     6181 ggcgcccgct gctccagcct gcctcggaat taattcagct tagcgtactc tgccttggct
     6241 tgcttcagga tggggatgtc agcgtcggcg tctttccaga gggtgaggaa atcggtatac
     6301 gctgcgcgag ccttggccgt gtcgccttgc agggcatacg ctctgccaat ttggagatgc
     6361 gcgagtgccc cgatgacggc gtctcccaca attccccggt ggtcaagaat tttctggaac
     6421 tcgcccgcgg cttcgctgcc ccgatgtgcg gccagatagg cctcgccgcg
//
LOCUS       DATNDT010000061         4820 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3630783, whole genome shotgun sequence.
ACCESSION   DATNDT010000061 DATNDT010000000
VERSION     DATNDT010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3630783"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..624
                     /locus_tag="VN788_01835"
     CDS             <1..624
                     /locus_tag="VN788_01835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19303.1"
                     /translation="GGARLLLPQLTLLKVWDTDGTRSENRSAYASCRAFQSESVFRPD
                     VAPFAGDPASGKALSVDDSATVKTPLAIPSGSILQIALRSKIDSESAFAGDPIEGQLL
                     QAIRDRGGNILASPGTVVHGRIVRFEQHYAPSSYFALGLKYYSLVVNGSEVPLTLEPI
                     PQSPGERDLTGSLEKRQGIGMFMFQSQRLALDDTFVSEWKTKKAKTR"
     gene            655..1311
                     /locus_tag="VN788_01840"
     CDS             655..1311
                     /locus_tag="VN788_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HXU19304.1"
                     /translation="MFKSLIAALAISCCASTAAFSAEWKQLFDGKDLNGWQHVGPGGD
                     TVENGLIRTHGGMGLLYWKGGKIGNCMLRVVFKMRDHNDNSGVFVRIPVEPREAWMPV
                     NYGYEVQIDNEPEKGGENEYHVTGTLYSLTKPLARPGKPGPEWNTMDITLDGPRTIVF
                     VNGVKVTDYTEGQPVPPKKFSFEPERGPRPLTGWFGLQNHSNNDIVFFKEVAIKPLGK
                     "
     gene            complement(1400..2122)
                     /locus_tag="VN788_01845"
     CDS             complement(1400..2122)
                     /locus_tag="VN788_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXU19305.1"
                     /translation="MSNFLVSGGNPLHTSSMSVLRAVPLALVLSMLVAAQQTTSFPTE
                     DGGRVCADVYGQGTRAVVLAHGGRFNKESWREQARALVSEGFRVLAIDFRGFGCSTGP
                     GQADFDHAPFENDVIAAVHYLKVHDAKTVSVIGGSFGGGAAGDASIKSAPGEIDRIVF
                     LGAAPNLSAEKLKSRALFIVARDDANDSGPRLPGIRAQYEKAPQPKQLIILDGSAHAQ
                     FLFQTDQNARVMREILRFLSKP"
     gene            complement(2219..2980)
                     /locus_tag="VN788_01850"
     CDS             complement(2219..2980)
                     /locus_tag="VN788_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19306.1"
                     /translation="MSKKIFTRWAAASAIALLGLMTAASARAQDQTYYTFVSEWAVPR
                     AQWVDFEKAAEQANSILRGLVTDGTLVAWGSEAELVHTESGYTHADWFVSTSQGGIMK
                     TLEALRSLSQGPAFTSAVKHQDAMLHTIAHGGKTAQATSGYIRVSFWQAKPGRGDEVE
                     QFFKKYMQPDLDAGVASGSVLMYNFEDQQIHTDAPGDYYLAIVYANGDGLDKGSAILA
                     AHAKDNPAAVAEFSAMVDIGTHRDSLGRILAFQHK"
     gene            3583..3957
                     /locus_tag="VN788_01855"
     CDS             3583..3957
                     /locus_tag="VN788_01855"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXU19307.1"
                     /translation="MRKKDDSVRYTAEEVKAKVARRGRTNWAKADSIAALELEASIRA
                     DVDDVQSDPDWTQAVMGIPFLKDHVNIRIDHDVLAWFKSNGRGYQTLMNNVLRAFVQS
                     RQSRQHMKSKQPSTRGRTQGFI"
     gene            complement(4216..4503)
                     /locus_tag="VN788_01860"
     CDS             complement(4216..4503)
                     /locus_tag="VN788_01860"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXU19308.1"
                     /translation="MASSTGTMAGIQFDALRYQEGRRWELLEGDLVEVSSPTPEHQDI
                     VFNLLAALKQYLKEQSVGRAHQDVEFALSRQDRLPAGCMRTAGRAGQFRTA"
     gene            complement(4534..>4820)
                     /locus_tag="VN788_01865"
     CDS             complement(4534..>4820)
                     /locus_tag="VN788_01865"
                     /inference="COORDINATES: protein motif:HMM:NF023385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3473 domain-containing protein"
                     /protein_id="HXU19309.1"
                     /translation="RLNREGHSACIYFHPWEIDKDQPRLECGFIGRLRTYTGIRGMGK
                     KIARLLKDFSFSTAGEVFPIPSEYPLSSPNLAPPAKRPRSETSKPVRAAV"
BASE COUNT         1021 a         1359 c         1420 g         1020 t
ORIGIN      
        1 ggcggcgccc ggctgctgct tccccaattg acgcttctga aggtctggga cactgacggc
       61 acaagatcgg aaaaccggag cgcttatgct tcctgcaggg cgtttcagag cgaatcggtt
      121 ttcaggccgg acgtagcgcc gtttgccggc gatcccgcat cggggaaggc gctgtccgtc
      181 gatgattccg caacggtgaa aacgccgctg gccattccgt ctggaagcat tttgcagatt
      241 gctttacgct cgaagatcga ttcggagagc gcattcgcgg gcgatccgat tgaagggcag
      301 ttactacagg ctatccgaga ccgtggcggg aacatcctcg cttcgccggg cactgttgtg
      361 catgggcgga tcgttcgttt cgagcaacac tatgcgcctt cgagttactt cgcgctgggt
      421 cttaaatatt attcgctcgt ggtgaacggc agtgaagttc cgctgacgct ggagccgatt
      481 cctcaatccc ccggagagcg ggacctgacc ggttccctcg agaaacgcca gggcatcggg
      541 atgtttatgt tccaaagcca gcggttggcg ctcgacgaca cgtttgtttc ggaatggaag
      601 accaaaaagg cgaagacccg atgataggat atttcctgtt cctctaaatt caccatgttc
      661 aaatcattga ttgcggcgct cgcgatttcc tgctgcgctt cgacggctgc cttttccgcg
      721 gaatggaaac agcttttcga tggaaaggac ctgaatggct ggcagcatgt cgggccgggc
      781 ggcgatacgg tggaaaacgg gttgatccgc acgcacggcg gaatggggct gctgtactgg
      841 aaaggcggga agatcgggaa ctgcatgctg cgcgtggttt tcaaaatgcg cgaccacaac
      901 gacaattcgg gcgtgttcgt gcgcatcccg gtcgaaccgc gcgaggcctg gatgccggtg
      961 aattatggct acgaagtgca gatcgacaac gagccggaaa agggaggcga gaatgaatac
     1021 cacgtcaccg gcacgctcta ctcactgact aaaccgttag cgcgtccggg taagcccggg
     1081 cccgaatgga acacgatgga cataacgctc gacggcccgc ggacgattgt gtttgtgaac
     1141 ggcgtgaaag tgaccgacta cacggaaggc cagccggtac cgccgaagaa gttcagtttc
     1201 gagccggagc gcggtccgcg ccctctgacc ggctggttcg ggctgcagaa tcacagcaac
     1261 aacgacatcg tatttttcaa agaagtcgcg atcaagccgc ttgggaagta gatagcgcgg
     1321 cggggatcct gccactcctg cgaccgccaa gcccgatgtg gaaactttac gcccgaccgg
     1381 aattccggaa ggtaaacaac tatggtttcg acaggaatcg tagtatttcg cgcatgaccc
     1441 gagcattctg gtccgtttgg aaaagaaatt gagcgtgcgc agaaccatcc agaatgatta
     1501 gctgcttcgg ctgaggagct ttttcgtact gcgcacgaat gcctggaagg cgcgggccgc
     1561 tgtcgttggc atcatcgcga gcgacgatga acagggcgcg tgacttcagc ttttctgccg
     1621 agaggttagg cgccgccccg agaaacacga tgcgatcaat ctcaccggga gcggacttga
     1681 tcgacgcatc gcccgcagct ccgccaccga agctaccccc aattaccgac acagttttgg
     1741 catcatgcac tttcaagtag tgaaccgcag cgatcacgtc gttctcaaag ggggcgtgat
     1801 cgaagtccgc ctggccggga ccggtcgagc agccgaaccc acggaaatcg attgccagga
     1861 ctcgaaaccc ttccgatacg agagcccggg cttgctcccg ccagctctcc ttgttgaatc
     1921 tgccaccgtg agcgagcaca acggctcgag tgccttggcc gtagacatcg gcgcaaactc
     1981 gtccgccgtc ttcggtagga aacgaggtgg tttgctgtgc ggccaccagc atagaaagga
     2041 cgagcgcgag gggcacagca cggaggacgg acatgctgga agtatggagc ggattgccgc
     2101 cgctcacaag gaaattgctc acacatccaa caagccatga ttctttcatc tggccgtatt
     2161 aaggctcgct gacggctctc acgctacgcg agcgagataa taggcgtcat tcgcaaacct
     2221 atttatgctg gaacgcgaga atccttccga gactgtcgcg gtgtgttcca atgtcgacca
     2281 tcgcactgaa ttccgcgact gccgccggat tatccttcgc gtgcgccgcc agtatggcgc
     2341 tgcccttatc caatccgtcg ccgttcgcgt aaacgatcgc cagatagtag tcccccgggg
     2401 catccgtgtg aatctgttgg tcttcaaagt tgtacatgag cacggaccca ctggccacgc
     2461 ctgcatcgag gtccggctgc atgtacttct taaaaaactg ctcgacctca tcgccccggc
     2521 cgggcttcgc ctgccagaag ctcacccgaa tgtaaccgga agtcgcttgc gcggtcttac
     2581 cgccatgggc gatggtatgg agcatcgcat cctggtgttt tacggcgctg gtgaacgccg
     2641 gaccttgcga caagctccgt agggcctcaa gtgttttcat tatgccgccc tggctggtcg
     2701 agacgaacca gtctgcatgg gtgtagccgc tttcggtatg gacgagttca gcctccgatc
     2761 cccaggcgac cagcgtcccg tcggtaacca ggccgcgcaa aatcgaattt gcctgttctg
     2821 cggccttttc gaaatcgacc cactgggctc gcggcaccgc ccactcagac acgaaggtgt
     2881 aataggtttg gtcctgcgcc ctcgcggacg ctgctgtcat cagtcccaaa agcgcgatcg
     2941 cgcttgcggc tgcccaacgc gtgaagattt tcttgctcat cccattcctc cgaatgtgaa
     3001 ttaacccgta tcgcgcagcg ccagattcaa gcgaactgga tcagaattcc gctacgagtc
     3061 tcgggaaccg tagtataccg tcgattacaa tcgcggaagt tggtctttgg tattccatta
     3121 ccggcagtac ttcaccgtcg tccaaggaac aacggaacgt tttcacactg cccacgcggc
     3181 cggcttgagc ctcggtgcgt gctcgcggcc cgacgcggtt ttcgctgaac gacatcgtgg
     3241 ttttcaagaa agttgcgatc aagccgcttg ggagtagatg gcgcgggggc ggcacccctt
     3301 aagagcatca accccgcttg ttggtgcaat tgtggtgtga cgcggatttt atttgggatg
     3361 agccaaagcg cgaatgggtg ctggccgggc ggggaacaga ttttcttcgt atcgcctccg
     3421 atttgtttga cggcccgtcc gcttctcacg gcgcgcccac gatacgcgat gacgaggagc
     3481 aaattttgag cattggcccg cttgaaagca aattctttgc ggctgtatgg acttggcggg
     3541 aggggccgtg cgcatcattg cagacagcga ggagggcgag agatgcggaa gaaagacgat
     3601 tccgtgcgat atacggctga agaagtcaaa gcgaaagttg cgagacgagg ccgaacgaat
     3661 tgggccaaag ccgactccat tgccgccctc gagcttgagg catcgatacg cgccgatgtg
     3721 gacgatgtgc agagcgatcc cgactggacc caagctgtta tgggcattcc attcctcaaa
     3781 gatcatgtca atatccgcat cgatcatgat gtgttggcgt ggttcaagtc gaacggcaga
     3841 ggctaccaga cccttatgaa taatgtactt cgcgcttttg tgcagtcgcg gcagtcacgc
     3901 cagcacatga aatcgaaaca gccttcaacg cgcgggcgaa ctcagggttt tatttgaaca
     3961 gcgccgcaac tggcaggctc cagccgggca gcaattccgt tgtaaggctc tggttatcct
     4021 gaagtttgcg cccccggcgc cagcgtaaac tagcacttcc cgcgtgaccg ggtagatctg
     4081 ccatacctcg cgaactccgg acttcagata agcgagcact ttacgtcgag tgtcgccggt
     4141 ccgctccgtg gcccggtccg ctccgtgggc gagatcactt ccacggcaat atccggcgcg
     4201 ccatgggtcg gtattttacg cggttcgaaa ttggcccgct ctcccagcag tacgcatgca
     4261 tccggccgga agccggtctt gcctgctaag ggcgaactcg acatcctgat gggcgcggcc
     4321 gactgattgt tccttgagat actgcttcag cgccgccaag agattgaata cgatatcctg
     4381 gtgctccggt gtggggctgg atacttcaac cagatctcct tcgagcaact cccaacgccg
     4441 gccttcctga taacgcagag cgtcgaactg tatacccgcc attgtgccgg tggagcttgc
     4501 cataactgaa ttgtatgcca gcggaaaagc cgtttatact gccgcgcgga ccggttttga
     4561 ggtttcggaa cggggccgtt ttgccggtgg cgcgagattg ggcgaggaaa gcggatattc
     4621 ggatggaatc gggaaaacct cgccggcagt ggaaaagctg aaatccttca gcagccgcgc
     4681 gattttcttt cccatgccgc ggattcccgt gtaggttcgc aagcgtccta taaacccgca
     4741 ctctagccgt ggttgatcct tgtcgatctc ccatggatgg aaatagatgc aggcggaatg
     4801 gccttcacgg ttaaggcggc
//
LOCUS       DATNDT010000062         6990 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1050240, whole genome shotgun sequence.
ACCESSION   DATNDT010000062 DATNDT010000000
VERSION     DATNDT010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050240"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2227
                     /locus_tag="VN788_01870"
     CDS             <1..2227
                     /locus_tag="VN788_01870"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HXU19310.1"
                     /translation="ALCAALAILLGTAATLAGPRPSKALTVERIYDAPSLSGSLTEGI
                     EWEPDGKRISYLDHSGPEPAMWIMDAATGRRNVFVTADVLNQVTQPQATKAVQSTGLG
                     RVQAETYFWSPRNDALLFSGASSLVLLNLKTMEAKPLVNPSDEAGHEIADPKFSPDGR
                     WVSFVRDSNLWVANVATGQIKALTTGGSEEILKGQLDWVYPEELDATTAYWWSPDSSK
                     IAYYKMDERPVTRYPIVDMSSLTGAIEYTRFPEAGGANPVVRVGVVPVNGGETKWMDT
                     GTDTDVYLPRVVWLRNSSRVAIERLNRAQNRLDLLFCDASTGDSENILTETDKYWVNV
                     ADDLYFFADNKRFLWSSERTGFRHYYLYDLAGHQLAQLTSGKWAITGNGGVGPGAASH
                     PAVDEAHGFIYFLSNKDNVLDIQLYRLSLADKSITKVTREPGTHDVLIAPNESAFVDT
                     YSTAMTPPRQDLDRMNGALTAAIDENSVPELADYQLSPVKFLNLSTDDGTKLYASIIQ
                     PPDFDASRKYPVLVNVYGGPQVQNVHNAWDGKDFLWLEMMAEKGYIIFTLDNRGSYGR
                     GHAFETAIYHHLGKIALEDQLNGVKYLKSLPYVDPLRIGIWGWSFGGTMALDAMFDAP
                     DVFKAGVSVAPVTDWRLYDTIYTERYMGRPQDNPEGYSDSSPANEAANLRGKLMIAHS
                     TGDDNVHFVNTSEVLNKLILSGKYPAELMIFPGRGHGISDAPARIQLFLGITDFLRDN
                     L"
     gene            2343..3245
                     /locus_tag="VN788_01875"
     CDS             2343..3245
                     /locus_tag="VN788_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19311.1"
                     /translation="MALSGKAAQAAGCLIVISAFCCVASFLPCYAAGPQPNDSAEVPP
                     SPAALLDNGFRSLYELNFKGARADFLSYQQRNPTDAMGAAAEAASYLYEQFDAKGIFT
                     SQFFLNDDKFLHGADGTAAENCNTPFLTANNRARRMAKQTLKTEPKNAHALLVLTMAD
                     GMESDYDAMIVKKQMAGLGLMRQAEAEANKLLAIDPDANDAYLALGASNYVIGNLSGV
                     KRAFLWFGGIHGDKGRGIQQLQLAADHGHYLKPFAKIMLALAYEREHKLDLARPLLAS
                     LVDEFPSNPLYAHELSLIDHPGEP"
     gene            complement(3287..4756)
                     /locus_tag="VN788_01880"
     CDS             complement(3287..4756)
                     /locus_tag="VN788_01880"
                     /inference="COORDINATES: protein motif:HMM:NF024985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4139 domain-containing protein"
                     /protein_id="HXU19312.1"
                     /translation="MPSRITKIVSIAALAATVLISPGGKLIEVRAADSRGESQSKAEY
                     SATTQKDQSDLSVTVYNSNIALVRDVRQIRLQPGVFPLRFEDVAASIMPATVHFRSLT
                     DPSKLNVIEQNYEYDLLDPQKLLNKYVGKEVSFEWQGMQTKALLLSDNGGPVWKIGNQ
                     IVTGLSVGSYRFPELPDNLYSRPTLVWTLDNRGGDAQKVEASYLANSMNWNADYVLTV
                     ARDEKTADLDGWVTLVNNSGAAYHDAKLQLVAGDVHRTPEVMHEERDMVAKSMAMNAG
                     SMQFAQENFSEYHLYTLGRRTSIQNNESKQISLLTGTNIPVEKYLQVEGQQYYYRNPQ
                     GIGNAIPQPVKVFYRFKNEQQAGLGMPLPAGTVRVYQADSQGGAQFAGEDNIAHTPKD
                     ETLRIYVGNAFDVVCERKATDYKKIAYDLYEMEYQITLRNHKNGPVTVEVREPIGGDW
                     QVVNSNYKWTKLDVSTIGFQIPVAKDGTATLDYRVRVKW"
     gene            complement(4990..6222)
                     /locus_tag="VN788_01885"
     CDS             complement(4990..6222)
                     /locus_tag="VN788_01885"
                     /inference="COORDINATES: protein motif:HMM:NF026220.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YCF48-related protein"
                     /protein_id="HXU19313.1"
                     /translation="MASRPDKRPDKADTELLRQSLAHDPSFSAANGSCPEPEILAAYF
                     ERSLNAEETARCELHLSGCERCREELALMDRVGERAAIGGRFAGRRAWLWDWRWLAPV
                     AAALIIAAIWIAQRPLNPNRESQSRQPLVAMSQPSEPPMKESPARGAPRAAAPSSEVA
                     QNRALDKAQNSLSREMAQPSTNEKRRGIAAFDARRDADSNVAAKSAGARQSAPEERAG
                     RTQTESKALADALQTTPALPAANTPSALTGNAGGNVQAETDSSAQALKSTQQMAAPAK
                     PSLYAQKDEKAAPAPSQIGAAVRTSDPRVLWRKQEKGILKSEDGGATWHQADLPAANA
                     RIVALAAPSAQVCWLVGRDSLILLTTDGTHWQSIVPPVKTDFVQIVAENASSATLATA
                     EGLRFQTSDGGKHWHPIP"
     gene            complement(6284..>6990)
                     /locus_tag="VN788_01890"
     CDS             complement(6284..>6990)
                     /locus_tag="VN788_01890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU19314.1"
                     /translation="DFFMAQYRPELYRAARAIVGNAGGGESAARDLADSLCADLYGLR
                     ERGGKRQSLFDYFHGRSKLGTWLRAILARRHVDEIRRARRTESLDDDMGEPRADIAAL
                     ASAAASVAPDPQRASYLAILQVALAAALNVLDPRDRLRLAYYYVDDRTLAEIGRLLGE
                     HEATVSRKLERTRRDIRRSVESALRRDKKWSDAQVRVCLEYAAGQWPFDLTIALGRSG
                     ATGSAAAEPGALSARD"
BASE COUNT         1422 a         2145 c         1944 g         1479 t
ORIGIN      
        1 cgcgctttgc gccgccctcg caattctcct cggcaccgcc gcgactctgg ccgggcctcg
       61 gccctcaaag gcgctcactg tcgagcgaat ctacgacgcc ccgagtctga gcggctcttt
      121 gaccgaggga atcgagtggg aacctgacgg caagcgcatc tcctacctcg atcacagcgg
      181 acctgagccc gcgatgtgga taatggacgc cgccactggt cgacgaaacg ttttcgtgac
      241 cgccgatgtg ctaaatcaag tcactcagcc gcaagcaaca aaagctgtcc agtcaacagg
      301 cctagggcgc gtccaagccg aaacctactt ctggtcgccc cgaaacgacg ctctgctctt
      361 ttccggcgcg agcagcctgg tgctgctgaa tctgaaaacg atggaggcca agccgctcgt
      421 caatccatca gacgaagcgg gccacgaaat cgctgatccc aaattttctc ccgacggccg
      481 atgggtcagc ttcgtgcgag attcaaatct ctgggtcgcc aatgttgcca ccggccaaat
      541 aaaagcgctg accaccgggg gcagcgaaga aatcctgaag ggccaactgg attgggtcta
      601 tcccgaggaa ctcgacgcca ccaccgccta ttggtggtcg cccgactctt cgaaaatcgc
      661 ttactacaaa atggatgagc ggccggttac ccgctacccc atcgtggaca tgagttccct
      721 aaccggagcg atcgaataca cgcgctttcc cgaagcgggc ggagcaaatc ccgtcgtgcg
      781 cgtgggcgtc gttccggtca acggcggtga aaccaaatgg atggataccg gcaccgacac
      841 ggacgtctac ctgcctcgtg tagtgtggct gcgcaatagc agtcgcgtgg cgatcgagcg
      901 cctgaaccgc gcccaaaatc gcctcgacct tcttttctgc gatgcctcca ctggcgattc
      961 cgaaaacatt ctcactgaga ctgacaaata ctgggtcaat gtcgccgacg atctttactt
     1021 cttcgcggac aacaagcgct ttctctggtc cagcgaacgc accggttttc gccactatta
     1081 tctctacgat ctcgcgggtc atcagctcgc gcagttgacc agcggcaagt gggccatcac
     1141 cggcaacggc ggcgtcggtc ccggtgccgc cagccatcct gctgtcgatg aagcgcatgg
     1201 cttcatctat tttctctcca acaaggacaa cgttctcgat attcagctct accggctgtc
     1261 gcttgccgac aaaagcatca ccaaagtgac gcgcgagccg ggcactcatg acgtgctcat
     1321 cgctccaaat gaatccgctt tcgtggacac ttattcaacc gcgatgacgc cgccgcgcca
     1381 ggatctcgat cgcatgaacg gcgctctcac cgccgcgatc gacgaaaaca gcgtccccga
     1441 acttgccgac taccagcttt ctcccgtgaa attcctgaat ctctccacgg acgacggcac
     1501 caagctctac gcctcgatca tccagccgcc ggatttcgat gcttcccgaa aatatcccgt
     1561 gcttgtcaac gtgtacggcg ggccgcaagt ccaaaacgtt cacaatgcct gggacggcaa
     1621 ggactttctc tggctggaga tgatggccga aaagggctac atcattttca ctctcgataa
     1681 tcgcggctcc tacgggcgcg gccacgcatt tgaaaccgct atctatcacc atcttggaaa
     1741 gatcgcgctc gaagatcagc ttaatggcgt gaaatatctg aagtcgcttc cctacgtgga
     1801 ccctttgcgc atcggcattt ggggctggag tttcggcggc accatggcgc tggacgccat
     1861 gttcgatgcg ccggatgttt tcaaggccgg cgtttccgta gcgcccgtaa ccgactggcg
     1921 gctctacgat acgatttaca ccgagcgtta catgggccgc ccgcaggata atcccgaggg
     1981 ctacagcgat tcctcgcccg cgaatgaagc cgcgaatctt cgcggaaaat taatgattgc
     2041 gcattccacc ggggacgata acgtgcactt cgtgaacaca tccgaagtgc tcaacaagct
     2101 catcctctcc ggtaaatacc cggcagaact gatgatcttc cccggacgcg gccacggcat
     2161 ttcagacgcg cccgcgcgca ttcagctctt cctgggaatt actgattttc ttagggataa
     2221 cctgtaggca ccaaaaccca tgcaactcct ggcctcccgg aacagtcgaa ctagaatgag
     2281 ggtagcaggc gaaaacttaa agcgggctcc agggggcaaa taggtttatg cttcgaggta
     2341 ctatggcgtt aagtggcaag gccgcgcaag cggcgggatg cctgatagtc atttcggcat
     2401 tctgctgcgt ggcgtcgttc ttaccgtgct acgccgctgg cccgcagccc aacgattccg
     2461 ctgaagtccc gccatcccct gccgccttgc tcgataacgg ctttcgcagt ctgtacgaat
     2521 tgaatttcaa gggcgcgcgc gcggattttc tctcttacca gcagaggaat cccactgacg
     2581 cgatgggcgc cgccgccgaa gctgcgagct acctctacga gcaattcgac gccaagggaa
     2641 ttttcacatc gcaattcttc ctgaatgacg acaagttcct gcacggcgcg gacggcaccg
     2701 cggctgagaa ctgcaacacg ccgtttttga cagcgaataa tcgcgctcgc aggatggcca
     2761 agcagacgct gaagaccgag ccaaagaatg cgcatgccct acttgtcctg acgatggccg
     2821 acggaatgga gtcggactac gacgccatga tcgtaaaaaa gcaaatggcc ggacttggct
     2881 taatgcgcca ggcggaagcg gaagccaaca aattgctggc gattgatccg gacgccaacg
     2941 acgcctacct cgcgctcggc gccagcaact acgtgatcgg caacctgtcc ggcgtcaagc
     3001 gcgcgttcct gtggttcggc ggaatccatg gcgacaaagg ccgtgggatc cagcaattac
     3061 agcttgccgc ggaccacggc cactacctga agccctttgc caaaattatg ctggcgctgg
     3121 cctacgagcg cgagcacaag ctggaccttg cccggccgtt gctcgccagc cttgtggatg
     3181 aattcccatc gaatccgctt tacgctcacg aactctcgct gatcgaccat cccggcgagc
     3241 catagccggt tttcgcgctt gcgctgctcg ccggagtgcc tcgcgcttac catttcactc
     3301 gcacccgata atccaaagtt gccgtcccgt ctttcgccac cggaatttgg aaaccgattg
     3361 ttgaaacatc cagcttcgtc catttgtaat ttgagttcac cacttgccaa tcgccgccga
     3421 tcggctcgcg gacttccacc gtcaccggcc cgttcttgtg attgcgcagc gtgatttggt
     3481 attccatttc gtaaagatcg taggcaattt tcttgtaatc ggtcgccttg cgttcgcaga
     3541 ctacgtcgaa tgcgttgcca acatagattc gtaaggtttc gtccttcggt gtgtgcgcga
     3601 tgttgtcttc gcccgcaaat tgcgcgccgc cctgtgaatc ggcttggtag acgcgcaccg
     3661 ttccggcggg cagcggcatg cccaggcccg cttgctgttc gttcttgaag cggtagaaaa
     3721 ctttcacggg ctgaggaatc gcgtttccaa ttccctgcgg attgcggtag taatattgct
     3781 gcccttcaac ctgaagatat ttttcgacag gaatattcgt tccggtaagc aaactgattt
     3841 gctttgattc gttattttga atcgaggtgc gccgccccaa cgtgtagagg tgatattcgg
     3901 aaaagttctc ctgcgcgaac tgcatgctcc ccgcgttcat agccatgctt ttcgcgacca
     3961 tgtcgcgctc ttcgtgcatt acttccggcg tgcgatgaac gtcgccggcc accaactgca
     4021 atttcgcgtc gtggtaggcg gcgccgctgt tgttcaccag cgtgacccat ccatccagat
     4081 ccgcagtctt ttcgtcgcgc gcgaccgtca acacataatc ggcgttccaa ttcatactgt
     4141 tggcgaggta cgacgcttct actttttgcg cgtcgccgcc gcgattgtcg agcgtccaaa
     4201 ccagcgtcgg acggctataa agattgtccg gcaactccgg aaagcgatag gaacccacgc
     4261 tcagtccggt gacaatttgg tttccgatct tccacaccgg cccaccgttg tctgaaagca
     4321 gcagcgcctt tgtttgcatg ccttgccact cgaacgagac ttccttgccg acatatttgt
     4381 tcaatagctt ctgcggatcg agcaagtcgt attcataatt ttgctcgatc acattcagct
     4441 tcgaaggatc ggttagcgaa cggaaatgca ccgtcgcggg catgatcgaa gcggcgacat
     4501 cctcaaaacg caatgggaac acgccgggct gcaagcgaat ctggcggacg tcgcgaacca
     4561 gcgcaatatt tgagttgtag acagttacag aaaggtcgga ctggtccttc tgggtggtgg
     4621 ctgaatattc ggcctttgac tggctttcgc cgcgtgaatc ggcggcgcgg acctctatca
     4681 gtttgcctcc tggggaaata agaacggttg ccgccagtgc ggcaatgcta acaattttcg
     4741 ttatgcgtga tggcatgggc tgtctccttc gcgtaactgc tgcttcacgg ttagaacggc
     4801 gaggagcaaa tttcttgcag tctatttttg cggtgatctg acgatggctt ttacaaacgt
     4861 ggcggctttt ctggggccgc gacagggggt ccgcaggtgg aagcgcaggc cttcagctgc
     4921 gaatagcgtt aatccaaatc atggctttag caatgggggc gagctggatg cgttcggctc
     4981 acagaagtat taggggatgg gatgccagtg cttgccgccg tcgctggtct ggaatcggag
     5041 gccttcagca gtagcgagag tagcggagga tgcattctcg gccacaattt ggacaaaatc
     5101 cgtcttcacc ggcggcacaa tgctttgcca atgcgtccca tcggtagtga gaaggatcag
     5161 cgagtcgcgc ccaacaagcc agcacacttg cgcggacgga gcggcgagag caactatgcg
     5221 cgcgtttgcc gccggcagat cggcttggtg ccacgttgcg ccaccgtctt cggatttcag
     5281 aattcccttt tcctgtttcc gccagaggac cctcgggtcc gaagtccgca ccgcggcccc
     5341 aatttggctt ggcgccggag cggctttctc atctttttgg gcgtacaaac tcggtttggc
     5401 gggagcggcc atctgctgcg tcgacttcag agcctgcgcg cttgaatccg tttccgcttg
     5461 gacattccca ccggcattcc ccgtcagcgc agacggcgta ttcgcagccg gaagagccgg
     5521 ggtcgtctgc aaagcgtccg caagcgcctt ggattcggtt tgagttcgac cggcgcgttc
     5581 ctcgggagcg ctctgacgcg cgccagccga tttcgccgcg acgttcgaat cagcatccct
     5641 gcgagcatcg aaagctgcga ttccacgccg cttttcgttg gtcgaaggct gagccatctc
     5701 acgcgaaaga ctattctgcg ctttgtcgag cgcgcgattt tgcgccacct cggacgaggg
     5761 cgcagcagcg cgaggcgctc cgcgcgcggg cgattccttc atcggcggct ccgatggctg
     5821 ggacatcgca acgagaggct gcctcgattg cgattccctg ttcgggttca acgggcgctg
     5881 cgcgatccag atcgcagcaa tgattaacgc ggcagccacg ggcgccagcc agcgccagtc
     5941 ccaaagccat gcccggcgcc ccgcaaagcg acctccgatt gcagctcgtt cgccgacgcg
     6001 atccatcagc gccaattcct cgcggcagcg ctcgcaacca gacagatgca actcgcaacg
     6061 cgctgtttcc tccgcgttca gcgagcgctc gaaataagct gcgagaatct ccggctcagg
     6121 acacgagcca tttgcagcgc tgaaggaggg atcgtgcgcg aggctttggc gcagcagttc
     6181 ggtgtccgct ttatctggac gtttatcggg ccgtgaagcc atgaacctgt gctcccccta
     6241 tatcttagaa cggacgaaat cgcaaatctt gcagcatcga aattcagtcc cgtgccgaaa
     6301 gcgcgcccgg ctccgccgct gccgaaccgg tagccccaga acgtcccagc gcaatcgtca
     6361 aatcaaaggg ccattgacct gcggcgtatt ccaagcagac gcgcacctgc gcatcgctcc
     6421 atttcttgtc gcggcggagc gccgattcga cgctgcggcg gatatcgcgc cgcgtgcgct
     6481 ccagcttgcg cgagacggtg gcctcgtgtt caccgagcag gcgcccaatt tcggcaagcg
     6541 ttcggtcgtc cacgtagtaa taggccaggc gcagccggtc gcgcggatcg agaacattca
     6601 gcgcggctgc gagcgcgact tgcaatatcg cgagatagct cgcgcgctgc ggatccggcg
     6661 caacacttgc agccgccgat gcaagcgccg cgatatccgc gcgtggctcg cccatatcat
     6721 catcgagcga ctctgttctt cgagcgcgcc gaatctcgtc cacgtgccgc cgcgcgagaa
     6781 tagcgcgcag ccaggtgcca agcttgctgc ggccgtgaaa ataatcgaag agcgattgcc
     6841 gctttccccc gcgctcgcgc aagccgtaca aatcggcgca gagggaatcg gcgaggtcgc
     6901 gagctgcgga ttcaccaccg cccgcatttc ccacgatggc tcgcgccgca cggtaaagtt
     6961 ccggccggta ttgcgccatg aagaaatccc
//
LOCUS       DATNDT010000063         6508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3628605, whole genome shotgun sequence.
ACCESSION   DATNDT010000063 DATNDT010000000
VERSION     DATNDT010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6508
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3628605"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..287
                     /locus_tag="VN788_01895"
     CDS             27..287
                     /locus_tag="VN788_01895"
                     /inference="COORDINATES: protein motif:HMM:NF019016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HXU19315.1"
                     /translation="MIEFPNHSRSYDRTRRAVRFWGHDSAIEASFFINEDALKRIQPD
                     AGYDESGFLHAFDSNRDLICAAAAKVYVRGSRGSYDLVAANF"
     gene            complement(469..1197)
                     /locus_tag="VN788_01900"
     CDS             complement(469..1197)
                     /locus_tag="VN788_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19316.1"
                     /translation="MLVLVEAVIFYVADVDTLYDRALAAGYQPAAVPHDAEWGERFFH
                     QMTPMATNSVSFRLCSQFPSNSSSKRPAMRRCESVLCSPLNLGGNHGVQDNRTPVCSA
                     RWHFPPPGPHKHRRATGNYTNTVTDVRLHFKVVSITLRPDERSSVLAANGILYQMSGS
                     IEVSLGGEAKRLTAEEGLFIAGGKTAALTAGSGVPSTFLHFSQARSVRSSQPFRKAKR
                     PNRSKREKENSSTRQEGKRKICVQ"
     gene            2878..3177
                     /locus_tag="VN788_01905"
     CDS             2878..3177
                     /locus_tag="VN788_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19317.1"
                     /translation="MPQEKPEPDRIRELEDALKEAERRVAEMRAERDQAFELVEQMKE
                     HVSDVDTLIDSWIEAFEMQLSEDRKRTRDALPESIKKLLTDAVALQKQAKGLGKK"
     gene            complement(4071..4568)
                     /locus_tag="VN788_01910"
     CDS             complement(4071..4568)
                     /locus_tag="VN788_01910"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXU19318.1"
                     /translation="MSKRKPATASKHAHSTKIAAQRTNQAIIRSPKNHPLRAVAAGST
                     ESLPKRHNDSKQEAALVEKPEAPLVEKPATGLQDDCKQTMTNNDSKKRFAFPSSTPNV
                     RAYQAKLLEMAQANMQLAFEFAQRLATIRSPVEILSVIAEFTSKRIAMLQKYSIEIVE
                     LGTKR"
     gene            5754..6089
                     /locus_tag="VN788_01915"
     CDS             5754..6089
                     /locus_tag="VN788_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002712188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HXU19319.1"
                     /translation="MSNVEQLQKHAQEQNEAAMSSGNAFAENAKTITIAHAEYTRKAF
                     LDFSEFISKLSSLKSPDEAMKLQAEYARSAYETFVTDSKKISELYTDFAKQTFKPFEG
                     LIAKMTPAK"
BASE COUNT         1404 a         1834 c         1874 g         1396 t
ORIGIN      
        1 gtaccgacaa ctgatcggcc tcaaccatga ttgagtttcc aaatcacagt cgttcgtatg
       61 accgcacgcg gcgtgccgtg cgattttggg gacacgacag cgctattgag gcatcgtttt
      121 tcataaacga agatgcgctg aagcgaattc aaccagatgc tggttacgat gagtcaggtt
      181 ttctgcacgc gttcgattcc aatcgtgact tgatatgtgc cgccgctgcc aaggtctacg
      241 tccgcggcag caggggctct tacgatctgg tcgccgcaaa tttttgaacc gatttcgcac
      301 ctgcaagttc ggcctgatca gcgaaggcgc tcgacgaata gcgaaacgtc ggcccttcga
      361 tgtgcttgtc tgatgatctg atcacctaaa aaatcgcggc tgggccgacg tcagggtagc
      421 ggcagagtcg aaccgagact gcacccgatc atctctcttg ggagaatgtc attggacaca
      481 aattttacgc ttaccttcct gtcgtgtgga ggaattttcc ttctcgcgtt tgctgcgatt
      541 ggggcgtttt gctttgcgga acggttgcga ggagcgaacg ctcctcgctt gcgagaagtg
      601 gagaaaagtt gacggcactc cactgcccgc cgttagcgcc gccgtctttc cgccggcgat
      661 gaacagcccc tcttcggcgg tcagcctttt cgcctcgcca cccagcgaga cctcgatcga
      721 tcctgacatt tggtagagga tgccgttggc cgccaagacg ctgctcctct cgtccggccg
      781 aagagtgatg ctcaccactt tgaaatggag ccgcacgtct gtaacggtgt tcgtgtaatt
      841 gccggtggcg cggcggtgtt tgtgcggccc cggaggcggg aaatgccatc gcgccgagca
      901 aacaggagtc cggttgtcct gaactccatg attgcctccc aggttcagcg gagagcacaa
      961 tacgctctcg cagcgcctca tggccggccg cttcgagctg ctattggacg gaaactggga
     1021 gcaaaggcgg aacgaaaccg agttcgtggc catcggggtc atttggtgga agaagcgctc
     1081 accccattcg gcatcgtggg gcacggcagc tggctggtat cccgctgcga gtgcacggtc
     1141 gtaaagtgtg tcaacatcgg caacgtagaa aattaccgcc tccaccagga ccagcaccgc
     1201 tcggtaggct gggtgatgag gttgagatag ctcgttcctg ctcgaaagct ggtgaatgac
     1261 gaatcttcgc cgccatgcat gacctcaaac cccgcgcgcg gtagaatcgg aggctcgtgg
     1321 cattaagtgg ttgctgagtg taaccgcgct gatcccctcg atcatggata gactctcccg
     1381 tctcatttcg ccgcgccgtt gccacaggcc ccagactggc aactggcggc ggacgctcgc
     1441 cgcatcgggc gggagcttcc ggttccgcaa tttccgcggg gcgcttcctt ccagatatct
     1501 gcgaattaac tcgcgtttga gatccctcag gcgcatactc acacaatcac cgctccgcct
     1561 cctgggcatt accggtgacc gagagtgttg cgctcccgcc tgttcgaggg aagcatcatg
     1621 ccccaatctc aacgcctctc gtccgtcatt ccattcgctg caattaagcg ccctgcctgt
     1681 ccgaagtgca aggcccagat gatgctcgtg agcatcgagc cagcacgcgc ccagggcgtc
     1741 gacctgcata cgtttgaatg cgctgtctgc aatcaggtac ttaaaccttt gccgcgtatg
     1801 aagaccccat gaagtccaag ggccttggac gctggcttca gggcgaattg tgcccgccaa
     1861 cgtaaggccc gccgggcggc gatgggtcgc ctctcctcgt ccggatgagc acttcggtga
     1921 agtccacgcg ggcgcgttgc catgaacgtt ggggcgatcc ggaacggaag ctccagccat
     1981 aagcataatt cctcttcacg ttcagagacg cttcgagcag cgatgggccg ctcggttcgg
     2041 ttcaactgac aatccccgcc gtaccaagtc ggattgaaag gctcgccggt caacacgcgc
     2101 cgcgcgcggc aaaagccaaa gaaaaacccg ccgggccgag ggtgaaagcg cacggaaaat
     2161 gacggacgac aagaaaagca ccgatcgaag tctgtctgtc gctgagcgaa aaactcttag
     2221 gggacacgag gcacgagcgg ccattgcgga tcatgaaccc gctcagaagg cgttcaataa
     2281 aaatccgaat cggctacggg aagaacgcga gatggggccg cgggtccgat ggtcgctccc
     2341 acacccgagc taccggacca tatgccgatc aaacgcgtcc tatttcccac cgggatccag
     2401 aacgctctcc gaggagccag acctgaaaac cgtcggtgag gtgcgcgaga tatcgggcga
     2461 ggcccttact agcctccccg attttggaag gcgctcgctc tccgatctcc gcatgaaatt
     2521 gggcctgccg tcgacccacg gagttagacc aataaggccc acctgattac ggcgatcgca
     2581 aagccttcag gctatacacc tcctcttccg gatggtgccg gccctgactc ccggaacgcc
     2641 gccggcgcgt ctttgcccca aagactcagt ccccgtcgaa atgaatcctc agggtcaggc
     2701 aggtggcaag gatcggattg cgcgggaacg aatccgggtg gcggtcgatt tcccccaaag
     2761 gccgggccgt gctctacttc gggcggcctt cgacatatgc tttccaccgc gaatacttga
     2821 cgcaaagagc ccccggatcg gcggacctga cgcctctccg gggagaaagc ccattacatg
     2881 ccacaagaga aaccagagcc ggaccgcatc cgagagcttg aggatgcgtt gaaggaggcc
     2941 gagcggcgcg tcgccgaaat gcgggccgaa cgcgatcaag cgtttgagct tgtcgagcag
     3001 atgaaagagc acgtcagcga cgttgacacc ttgatcgata gctggatcga ggcattcgag
     3061 atgcagctca gcgaggatcg caagcgaacg cgcgatgcgc tgccggagag tatcaagaaa
     3121 cttctcaccg atgctgttgc cttacagaag caggcgaagg ggctcggcaa gaagtaagat
     3181 gatcccggtc ccgcaccgca aaaaacgcga cggcggaaaa ctgcaagaac cggaagagag
     3241 gagtcggcac cttctgtgtt gctcgccgtt tagtcgcagc ggcgctcctt gcgcgtgatc
     3301 ctgcggccgt catcccgttc aacatggtag tcaccatgcg gcagcgctcg tggcggtctc
     3361 gcggtctcgc ccgacctgaa catcgacgcc gccttgggca atcttgacac cttgggcctt
     3421 ggccggagtg atgaggcagg gcagtcgtca gcatcggggc gctgccccga gcgacttgtc
     3481 gatagaattc ccgactagct tctggctagc agccaatttt gcagacttgg tctcacggtc
     3541 ctaccctcac tgcttgctcg tgccgacgag gcgatcgaat gagcggcgaa ctgctgtggc
     3601 gtatgtcttt tgaatggcag atgttgtgcc aaaagtcgaa cgttgcaggg ctcaagattt
     3661 tccgcgaaaa cgctaagcag cgagcgatcg ctgtgacctc aatcgcgtta ccgaagtcgc
     3721 ttgtgaattt tgcgtgagac cgttagatcc ctcacatctc gtgcacgaaa gtcgcgccca
     3781 agggctcgca gaattttgat cttcggcgaa aaaacttgtg agattttgcg aggtgctgat
     3841 ggcggccact gatctgctgc gcgtgatcag ttcgaagtcg gaagacttca cctttagtgg
     3901 cgccgtccga cggcggtgtt agacttcggc atggcatggt gctccttctg ctgccccaca
     3961 caagcatgct ggctcgcagg gcgggagcat ggccggtcca acccattctg agacatcagc
     4021 aacggtcgtt tgctgacatc gactcgctgg attccgtcac ggatgttttt tcaccgcttg
     4081 gtacctaatt cgacaatttc tatcgaatac ttctggagca tggcgatccg cttgcttgta
     4141 aattctgcga tgacgctgag aatttcaacg ggagacctaa tcgtcgcaag cctctgagcg
     4201 aattcaaaag ccaattgcat gttggcttgt gccatttcca gcagctttgc ttgataagcc
     4261 cgcacgtttg gcgtggatga aggaaaagca aacctcttct ttgaatcgtt gttcgtcatc
     4321 gtctgtttac agtcatcttg taagcctgtc gccggtttct caactagggg agcctccggt
     4381 ttctcaacaa gggcggcctc ttgttttgaa tcgttatgcc gcttagggag cgattcagtc
     4441 gagcctgcgg caaccgcgcg cagggggtga tttttcgggc ttctaataat agcctgattg
     4501 gtccgttggg cagctatttt cgtgctgtgg gcgtgctttg acgccgtcgc cggtttgcgt
     4561 ttgctcatct gacactccaa gggatgattg ggcgaccaca acggtcattg gtgtgcagcg
     4621 acaacatact gtggagtgta acgttccaaa cgcagattaa attcaccgca taggctgatc
     4681 aggaagtttc ggcgatatgc gcgacgacgc tgaatgcgtt gtgtggtgag cgttgcctta
     4741 tttaactttt cccgatgtga gcaatattga ccaccagctc cggaaccgaa cttttctgag
     4801 aggtggtctg tattgaccag catgtcgggg cgcgggcgtg gaagactatt cccatcaccg
     4861 cccgaaacgg cagctaccgg tgactgagag tattgtgctc ccgcctgatg taagggacga
     4921 catcatgagc gcgttttgca gaccaccatg tccgacatgc aaagcgataa ctatgctggc
     4981 acggattaca cctggcccat ccggctttga catcagaaca ttcgaatgct ccgcttgcga
     5041 tcatgttcat cagcgcgtgg tggggcttgc cgatccgata aaacctctgg aaacagtagg
     5101 ctggctccgc ggcgaattgc gagcgccaac gtaggggctc agttgtcggc ctgtttcatt
     5161 tctcttcaat cgcggaaggg agagcgccat gaggcgtcga tccgaaccta acacattcga
     5221 cgagaggctg agcacagaag aggccctcat aacagcggca ttggaaaaca caggcccggg
     5281 cccgcggcgc aacttgcttg aacttaagct tcggcggatc gaaaccgccc gtcttatcgg
     5341 cggctgggtc tcaccgacgg gggtgcgccc ttcacccgga acaaggttgg cgcgtcctaa
     5401 gtccccggag tgagcaatat tatacagacc gccccgagag cttctgctta agtcgaccaa
     5461 ttgtcccgcc cttagtactt gcattcagcc cggcctcgcg ccgggctttt gcttgtgtgc
     5521 cccagttcgt ttcggcggat gtcaatgcaa cggccccgac gcggggttgg accgcgccgg
     5581 agtcatccag ggaacgcagc gcgggacggc gggtgatgac gcggcgttcg tatgcggctt
     5641 cttgcgtgtc ccagatgacg cgatttgatc agtagcaccc cttgaaatta tgctgcagcg
     5701 caccacatgt aaggttcaca atccgatcct ctagaggacc gagaaagagt ttaatgagca
     5761 acgttgagca attgcaaaag cacgcccaag agcaaaatga agcagcgatg tcatcaggaa
     5821 atgcgtttgc ggaaaacgct aagacgatca caattgccca tgctgaatac accaggaagg
     5881 cgttcctgga cttctcagaa ttcatttcga agctgagcag cctgaaatcg ccggatgagg
     5941 cgatgaaact tcaagctgag tacgcaaggt ctgcttatga gacatttgtt acagattcaa
     6001 aaaagatttc cgaactctac actgatttcg ctaagcagac gttcaagccg ttcgagggtt
     6061 tgatcgcaaa gatgactccc gccaagtaag agaagccacg taagagaagc tgcagctcgg
     6121 tcaaattagg tcgagctgtt cgatatggac cggccttcct tgctatgtcc ttcgatcaag
     6181 caacatgcgg agttgggtca ctcgcgtcgg actatgcgac gttccgagga agggttcatc
     6241 ctcgccgaag cagccgcatt tggtgaccac gtgcctcatg ccacaggagg cgaggtggaa
     6301 ccctgccttt agctcgatgt tgtcattcca gcaacggaac cggcctaact ggcagttgct
     6361 ccaaaagccg ctcggcgtaa tgcccgcgcg gctttttctt ttaacattgg gagggtaaca
     6421 tgccgaatga taagcccctc aagcccgacg actttccggt tgaggttgag ggcgaggaac
     6481 tagtgaccgc agacggcaag ccaatcgc
//
LOCUS       DATNDT010000064         6539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1632951, whole genome shotgun sequence.
ACCESSION   DATNDT010000064 DATNDT010000000
VERSION     DATNDT010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1632951"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..299
                     /locus_tag="VN788_01920"
     CDS             <1..299
                     /locus_tag="VN788_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19320.1"
                     /translation="MLNHLLSAVNPATLTQVLSGAGLSGLVGGGGAQLTPDEAQKVSP
                     EVVQQLAAHAEKANPSIIDSLSRFYAQHGTLVKTLGGAALTVALAKVAERQRQG"
     gene            350..601
                     /locus_tag="VN788_01925"
     CDS             350..601
                     /locus_tag="VN788_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXU19321.1"
                     /translation="MRLLWWIIVGLIAGWATGKIMKGGGYGTLMDIVIGIAGAIVGGW
                     IMSLLGFASAGGLIYTILVAIGGAIVLTALVRMFTKSRV"
     gene            702..1325
                     /locus_tag="VN788_01930"
     CDS             702..1325
                     /locus_tag="VN788_01930"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXU19322.1"
                     /translation="MRNLPKRIVLFAACSVLVFGAAAQTAFSAQTRAKTSKSETASAQ
                     KTGATVDLNTASEKELDSLPGVGPSTAKKIIAGRPYSSIDGLSKAGVSSATIKKIAPL
                     VTVGGGRAATASESSKKPSRKTAKETQPEPTPATPSQPPPPPKTTSATTSRGTPGAGT
                     VWVNLDTGIYHYSGSRYYGKTKNGKYMSEAEAMKAGYHAAKREKKPQ"
     gene            1340..1675
                     /locus_tag="VN788_01935"
     CDS             1340..1675
                     /locus_tag="VN788_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015862100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HXU19323.1"
                     /translation="MIRKRILSAATLFALAFLFLAPIGRGQSKPTHKGTTASSQASSA
                     ANADLVDINSATKDQLDALPGIGATYSQKIIDGRPYKAKTDLVRKKIIPQATYNKIAK
                     LVIAKQPAK"
     gene            2004..3038
                     /locus_tag="VN788_01940"
     CDS             2004..3038
                     /locus_tag="VN788_01940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HXU19324.1"
                     /translation="MATTKVGIAPMKVAQISKAGGDFEIVEREIPNPEAGQVRIKVQA
                     CGVCHSDSITKEGFLPGISYPRVPGHEIAGVIDELGASVTQWKKGQRVGVGWHGGQDN
                     TCPSCRRGDFGTCANLKICGISYDGGYQEYMVAPVEALAAMPESLNAAEAGPLMCAGI
                     TTFNALRHSGAMPSDLVAVQGIGGLGHLGIQFAKKFGYRVAAVGRGAENAALAKKLGA
                     HVYIDSAATDAAAELQKLGGARAILATAPNAKAMSSLIGGLAPNGVLMVVGAPSEPME
                     VSVLQLILGRRRVQGWPSGIPTDSEDTLRFAELAGVRPMIETYPLEKAAEAYARMMSG
                     KAQFRVVLTM"
     gene            3110..4735
                     /locus_tag="VN788_01945"
     CDS             3110..4735
                     /locus_tag="VN788_01945"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXU19325.1"
                     /translation="MRVLLVNLGAESSEAVKQALSGQGYEIPTAQTQAVDEVLGFSPE
                     VLITEATPSDLSCCGLISQIKANPQTLKIVMIVHGGALERARALDLGADDVISFPFER
                     LEFAARIRAQFRERQPGLELEAKLREAMQRRRMAEATAEALRGRNAKRRYWLIPAIFV
                     LGAAAVVAGVSTVISNRHSRKDTLQLKAEIARLNGGLLQQGELLSRVEQDRASLKASD
                     AGATRESLKAKTVAIRKRIAASGDADDESLEKQLQVTENRLNRLENESRIAETIVRTY
                     GPSVCLLHVVVAFRDKNSGRVIRVTTDANGKPEVDDKGMMSLQTEGTGPPLQLDVFGT
                     GFLVGRDGRLLTNHHVAEPWWGNKELKPLLDRGATAFVVSYTAYFPDASEGIAAEVEQ
                     ISPDADLATLKLQNAAPAHAAVLKLDDRSQASVTGDAVVLIGYPTGIDGILARAGGDV
                     TRKLAENAQNVTEIVTQLAAQHLIRPTTTQGHIGDVLKDKIVYDAATTSGGSGGPLFN
                     REGKVIGVNFAILTDFGGSNLAVPVRYADGLVK"
     gene            complement(4756..5091)
                     /locus_tag="VN788_01950"
     CDS             complement(4756..5091)
                     /locus_tag="VN788_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012427285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HXU19326.1"
                     /translation="MKITCFIRYQIDPLQRDGFKKYAENWGRIIPRCGGHLVGYFLPY
                     EGTNDVAWGLISFDSLAAYEAYKARLRSDREARDNFAMAQNRRLILREERNFVEVVEG
                     TSNLPSTLA"
     gene            complement(5161..6099)
                     /locus_tag="VN788_01955"
     CDS             complement(5161..6099)
                     /locus_tag="VN788_01955"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXU19327.1"
                     /translation="MATMKAVRIHEYGGPEALRYEDAPRPQPSSGEVLIRVHAAGVNP
                     VDWKVRQGLAKERLKYKMPFIPGWDVSGVIEAVAPGVTRLKPGDEVYSRPDISRDGSY
                     ADYIVVKESEVALKPKSIDHVSAAAIPLAALTAWQSLFDAAKLTSGQTVLIHGAAGGV
                     GTFAVQFAQLNGAHVIGTASKKNHDFLRSIGADELIDYNTTKFEDVVHDVDVVLDTIT
                     GETEARSYNVMRKGGIYVSILMPPSPEKAAQHGVRCAHTFVQANAEQLSEIAKLVDSG
                     KVKPIIEKVFPLAEARAAQESNATGHTRGKIVLRVV"
BASE COUNT         1488 a         1687 c         2058 g         1306 t
ORIGIN      
        1 gtatgctgaa tcaccttctc tccgcggtga acccagccac gctcacgcag gttttgtcag
       61 gggcagggct ctcaggattg gtaggaggag ggggcgcgca gctgacgccg gatgaggcgc
      121 agaaggtgtc gcccgaagtt gtgcaacagc tggcggctca tgctgaaaaa gcgaatccgt
      181 cgatcatcga ctcgctcagc aggttctatg cacagcatgg aacgctggtg aagacgcttg
      241 gcggagcagc tctaacggtg gcgctcgcga aagtggcgga acgccagagg cagggctgag
      301 ccgcgaggta tcggtcgagg tgcagtcgta agacgtggag gaaacgacga tgaggcttct
      361 atggtggatc atcgtggggc tgattgccgg ttgggctact ggaaagatca tgaaaggcgg
      421 cgggtacgga acgttgatgg atatcgtgat tgggattgcc ggcgcgatcg ttggtggctg
      481 gattatgtcg ctcttgggtt ttgcgagcgc cggcggtctg atttatacga tcctggtggc
      541 gatcggcggt gcgatcgtgc tgacggctct ggttcgaatg tttacaaagt cgcgtgtatg
      601 agctcctcga atgttgtgac ttacagacgt gcctttacga aggccttttc gacgcggtca
      661 ttcgcgtgtc aagggcagct tggctagaaa gcgagactaa aatgaggaat ttacctaagc
      721 gaatcgtatt gttcgcggct tgttcagtgc ttgtgtttgg cgcagcagcc caaacagcat
      781 tttcggctca gactagagcc aagacgtcca aaagcgaaac cgccagcgcg caaaaaacag
      841 gtgcaacagt agacctgaac accgcaagtg aaaaggaact ggatagcctg ccaggagtgg
      901 ggccttcgac ggcaaagaaa attatcgccg ggcgacccta ttcttctatc gatggtttgt
      961 cgaaggctgg cgtttcgtcc gccaccataa aaaagattgc cccgctcgtg acagtgggcg
     1021 gtggaagagc agccaccgcg agtgagtcga gcaagaagcc gagccgtaag accgccaagg
     1081 agacacagcc tgagcccact cccgcaactc caagccagcc acctccgccg ccgaagacga
     1141 cgtctgccac aacctcgcgg ggcacacctg gggcagggac cgtttgggtc aatctggata
     1201 ccggcatata ccactattcg ggcagtcggt actatgggaa gacgaagaat ggtaagtaca
     1261 tgtctgaagc tgaggccatg aaggcgggat atcacgctgc caagagagaa aagaaaccac
     1321 aatgagagga gaccgtttca tgattcgaaa acgaatatta tctgccgcaa cattgtttgc
     1381 tttagctttc cttttccttg caccgattgg cagaggccaa tcaaaaccca cccacaaggg
     1441 caccactgct tcgtcgcaag cctcgtcagc tgcaaatgcc gacctcgttg acattaattc
     1501 ggcgaccaag gaccagttgg acgcgctccc gggcatcggg gcgacgtact cgcagaaaat
     1561 catagacggc cgaccctaca aggcgaagac agacctggtg cgaaagaaaa tcattccgca
     1621 agcaacctac aataagatag cgaaattggt gattgccaag cagcctgcca agtagcggaa
     1681 cgagtccgac gatcacccaa cggtagcgag agcctgaccg ttcggcgtcg tgcggttgaa
     1741 atcacaacgg caaggcgcat gaagcggtcc tgggaggaga gccggcctgc tctccttggc
     1801 cacacccgag cgttgacgcg acaactcgca taggttgaac ccgcgacccg gtaggcgctc
     1861 ccaagggcct cgggtattca actccaaatt cccctattca caaccctcta gccgatatcg
     1921 agaattgcag cgcaatgtcc ggtcgctcca gcggtcgcaa tagggcttct caacaattgt
     1981 tgaaaacatt cagaggaggc attatggcaa cgacaaaagt gggaatagca cccatgaagg
     2041 tggcgcagat ttcgaaagcc ggcggcgatt ttgaaatcgt cgaacgcgaa attcctaatc
     2101 cagaggcagg gcaagtgcgc atcaaggtgc aggcttgcgg agtctgccat agtgactcga
     2161 tcacgaagga aggtttttta cccggcatct cgtatccgcg tgtccccggg cacgaaatcg
     2221 cgggcgtgat cgacgaactg ggcgccagcg ttacgcaatg gaagaagggc cagcgggtcg
     2281 gcgtaggctg gcacggcggc caggataaca cttgtccctc atgcagacgc ggcgatttcg
     2341 gcacttgcgc gaacctgaaa atctgcggca tcagctacga cggcggatat caggagtata
     2401 tggtcgcgcc agtggaagcg ctggcggcga tgccggaatc tcttaatgcc gcggaagccg
     2461 gtccattgat gtgcgcggga atcacgacat tcaacgcttt gcgccatagc ggcgccatgc
     2521 cgagcgacct ggtcgcggtg caaggaattg gcgggctggg gcatttgggg attcagttcg
     2581 cgaaaaaatt tggctatcgc gtagcggcgg tgggccgcgg agctgagaat gccgctcttg
     2641 cgaaaaaact tggcgcgcac gtttatatcg atagcgccgc cacggatgcc gcagcagagc
     2701 tgcagaaact gggcggcgcg cgcgcgattc tcgcgaccgc gcccaacgcg aaggcaatgt
     2761 cttcacttat cggcggtctc gctcccaatg gcgtcctaat ggtggtggga gctccatcgg
     2821 agccgatgga ggtctctgtc cttcagttga ttttgggacg gaggcgcgtt caaggctggc
     2881 cttcgggaat tcccaccgat tccgaagaca ccttgcgatt cgccgagctg gcgggtgtcc
     2941 ggcccatgat cgaaacctat ccgctcgaaa aagcggccga ggcttacgcg cggatgatga
     3001 gcggcaaggc gcagttccgc gtcgttctga caatgtaaac gctgtaatgg acatgccgtc
     3061 aagcgggtga aaattgacgg tagaaactgc gcgatgataa gttcgtagtg tgcgcgttct
     3121 tctggtcaac ctgggtgcgg aatctagcga ggcagtaaag caggccttat ccggccaggg
     3181 ctacgaaatc cccacagccc agacgcaggc ggttgacgag gtcctcggtt tttcccctga
     3241 agtcctgatc acggaggcaa cgccctccga cctcagttgt tgcggtctga tcagccagat
     3301 caaggcaaat ccgcaaacgc tcaaaattgt gatgatcgtt catggggggg cgctggagcg
     3361 agcgcgggcg ctggacctgg gcgctgacga tgtgatttcg tttccgtttg aacgactgga
     3421 attcgcggcg agaatccggg cgcagtttcg cgagagacaa ccggggctgg agctcgaagc
     3481 aaagctgaga gaggccatgc aaagacggcg catggccgag gcaacggcgg aagcgttgcg
     3541 cggcagaaat gccaaaagac gctattggct gattcccgcg attttcgttc tgggcgcggc
     3601 cgcggtggtg gccggtgttt cgactgtgat ttcgaaccgg cacagtcgaa aggacacact
     3661 gcaactcaag gcggagattg cgcggctgaa cggcggactc ctgcaacagg gagagctcct
     3721 gagccgggtg gaacaggacc gcgcatcgct gaaggcgagc gacgcaggcg cgacgcgcga
     3781 gtctttgaaa gcgaaaaccg tagcgatccg caagaggata gcggcgagcg gagatgcgga
     3841 tgacgagtcc ctggagaagc agcttcaggt gactgagaat cgcctaaatc ggctggagaa
     3901 tgagagccga atcgcggaaa caatcgttcg cacatacgga ccgagcgtct gtttgctgca
     3961 cgtggtagtg gcgtttcggg acaaaaattc gggacgggtt attcgggtca cgaccgatgc
     4021 caacggaaag ccggaagtag acgacaaggg aatgatgtcc ctacaaacgg aggggacagg
     4081 accaccgctg caactcgacg tctttggcac gggattcctg gtgggacgcg atgggcgctt
     4141 gcttacgaac catcatgtgg cggaaccctg gtggggtaat aaggaattga agccacttct
     4201 tgaccggggc gccacagcat tcgtggtgtc ttacacggcg tactttcccg acgcgtcgga
     4261 aggcatcgcg gccgaggtcg aacagatttc gccggatgcc gatctggcga ctctgaagct
     4321 gcagaatgcc gcgccagccc acgccgcggt cttgaagctc gacgatcgga gccaggccag
     4381 cgtaacgggc gatgcggtgg tcctgatcgg gtatccgacg ggaattgacg gcattctggc
     4441 gcgcgcgggc ggcgacgtga cacggaaatt ggcggaaaac gcccagaacg tgaccgagat
     4501 cgtcacgcaa ttggccgcac aacacctgat tcgccccacg acgacccagg gacatatcgg
     4561 agatgtccta aaggacaaga ttgtttacga cgcggcgaca acttcgggcg ggtccggtgg
     4621 ccctttgttc aatcgcgaag gcaaagtgat tggggtgaac ttcgcgattc taactgattt
     4681 tgggggctcg aacctggcgg tgcctgtgcg ctacgcggat gggctggtga agtagcagaa
     4741 gatgagtggc gcaggttatg cgagtgtcga gggcaggttg gaggttccct cgacgacttc
     4801 cacgaagttt ctctcctcgc gaaggatcag gcgcctgttt tgtgccatgg cgaaattatc
     4861 acgcgcttct cgatctgacc tcaatcgtgc cttgtaggcc tcataagcgg cgaggctatc
     4921 gaatgaaatc aaaccccaag ccacatcatt tgtaccttca tacggcagaa agtaaccgac
     4981 aaggtggcca ccgcagcgag gaatgatgcg gccccagttc tcagcgtact ttttgaaccc
     5041 gtctcgctgg agcgggtcaa tctgatagcg aatgaagcag gtaattttca ttttgtcctt
     5101 cccgtgttgg gggtcttact gctagaggtc atgaagcaga cgaaaaaatt gtggcacacg
     5161 ttagacgacg cggaggacga ttttgccgcg ggtgtggccg gtggcgttcg attcttgtgc
     5221 ggcgcgagct tccgctagag gaaagacctt ctcgatgatt ggcttcactt tgcctgaatc
     5281 gaccagcttg gcgatttcgc ttagctgctc cgcgttggcc tggacgaagg tatgagcgca
     5341 gcgcactccg tgctgcgcgg ctttttcggg cgacggtggc atgagaattg agacgtaaat
     5401 tccgcccttt ctcatcacgt tataggaccg agcttcggtt tcgccggtga tggtgtcgag
     5461 gaccacgtcg acatcgtgaa ccacgtcttc aaactttgta gtgttgtagt cgattagttc
     5521 gtccgcgccg atggagcgca gaaaatcgtg atttttcttc gaggcggtac cgatcacatg
     5581 tgcgccattt aactgcgcga actgcaccgc gaaggtgccg acgccgccgg ctgcgccatg
     5641 aatcagtacc gtctgaccgg atgtgagttt cgcggcgtcg aacagcgatt gccaggccgt
     5701 caaagcagca agagggatag ctgctgcgct aacgtggtcg attgatttcg gcttgagagc
     5761 gacttcggat tctttcacaa caatatagtc ggcgtaggag ccgtcgcggg agatgtcagg
     5821 gcggctatag acttcatcgc caggcttgag gcgggtgaca ccaggcgcga cggcttcgat
     5881 cacaccggaa acgtcccagc ctggaatgaa aggcattttg tatttgaggc gttcctttgc
     5941 aagtccttgg cgaactttcc agtccaccgg atttacgccg gcggcgtgca cgcggatgag
     6001 aacctcgccg gagctgggct gggggcgcgg ggcatcctcg tagcgaagag cctcagggcc
     6061 gccatattcg tgaatacgaa ccgctttcat cgttgccatc gtggaatcct tcaccgggtg
     6121 gtttatgcgc gggacataat agcaggctcc gccttttgcc gcatatggcg aagccgtggg
     6181 atggctactg gtagggccgc cgtcgccgaa gcatagaaca caaaggcgac aggctaagct
     6241 aggttgtttg agggtaataa agtagttctc aaggggtgct gacagcattc ggcaaataga
     6301 ttttattgaa ccggcaaggc catcgtgtta agttccgcgc aaagaagcaa gaacaggcgg
     6361 agtatgcacc tgtgcgaatc cgacatcgaa tctgaattaa gcgcgtgcca ctcgcaccgc
     6421 gataggaaat gggcaaggta aggagggacc aaaatgttcg aagctcaaac gaaggaaaaa
     6481 tcgggcggat cggggccgct gatcgtcatc tgtatcgttg tggtattggc ggtggtggg
//
LOCUS       DATNDT010000065         2075 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_770882, whole genome shotgun sequence.
ACCESSION   DATNDT010000065 DATNDT010000000
VERSION     DATNDT010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2075)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2075)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2075
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_770882"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1000
                     /gene="eutB"
                     /locus_tag="VN788_01960"
     CDS             <1..1000
                     /gene="eutB"
                     /locus_tag="VN788_01960"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HXU19328.1"
                     /translation="SKIRNVTRCRNTMGERGVLGIRVQPNHPVDDIGGILLAAFEGLL
                     YGCGDAVIGVNPASDSVEMVSAILHALNRLVETYKVPTQICCLAHIGAQLSAMDRGAP
                     VDLLFQSIAGTEAANRSFGISLSTLREGREKALEHHRARNAEWKGTQAMYFETGQGSA
                     LSAEAHQGVDQLTLEARAYGVARVFDPFIVNSVVGFIGPEYLYDERQIIRAGLEDHFM
                     GKLLGLPMGCDVCYTNHAAADQNSADNLLLLLGAAGCNYFMGVPCADDVMLNYQSTSY
                     HDALAVRRLFKLRPAPEFLVWLESNGIYRDAEPALLDAPARRQLMHGLESSLAEIA"
     gene            1001..1783
                     /gene="eutC"
                     /locus_tag="VN788_01965"
     CDS             1001..1783
                     /gene="eutC"
                     /locus_tag="VN788_01965"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020472889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HXU19329.1"
                     /translation="MSDKPDSSLAASDLPEILRKIRARTPARILMGRAGASYRTSTLM
                     DLREAHAAARDAVRAEFDLQKVFGAAFARQWQLFETRTRATSKEEYLLRPDLGRHFEP
                     ASQDEIRKRGSRESDLQIAIGDGLSVPAVVAQVPALLPLLHEGATARGWKIGRTFVIH
                     HCRVGILNEIGELLNPRVAVLLIGERPGLATAESLSAYVAYRPRATHSDADRNLISNI
                     HACGLNPHNAATRILNFAAQMIQAGSSGFTLRENLTALDNNK"
     gene            complement(1844..>2075)
                     /locus_tag="VN788_01970"
     CDS             complement(1844..>2075)
                     /locus_tag="VN788_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXU19330.1"
                     /translation="GLQGDQIPMMARIIAVADTLDAITTNRPYQSAMDLEYALGRIRS
                     LAVAKFDPKVVAALEAAVANGQLRLSATLVEV"
BASE COUNT          422 a          652 c          582 g          419 t
ORIGIN      
        1 atcgaaaatc cgcaatgtga cgcgctgccg aaacaccatg ggcgagcgcg gagtgctcgg
       61 catccgcgtg caaccgaatc atccggtcga cgacatcggc ggaattctcc tggccgcttt
      121 tgagggtttg ctttacggat gcggtgacgc ggtgatcggc gtgaatcccg cctctgattc
      181 cgtggaaatg gtcagcgcga tccttcacgc attgaatcgt ctggtggaaa cctacaaggt
      241 acccacacaa atctgctgcc tcgcccacat cggcgcccag ctctccgcca tggatcgcgg
      301 cgcgcccgtg gacttgctct tccaatccat cgcaggcacc gaagcggcga atcgcagctt
      361 tggcatctct ctctcgacgc tgcgcgaagg ccgcgaaaag gctctcgagc atcatcgcgc
      421 gcgtaacgcg gaatggaagg gcacgcaggc catgtatttc gaaacgggcc agggcagcgc
      481 gctttctgct gaagctcatc aaggcgtcga tcaactcact cttgaggcac gcgcctatgg
      541 tgtcgcgcgg gtcttcgatc ctttcattgt gaatagcgtg gtaggcttca ttggacccga
      601 atatctctac gacgagcgcc agattatccg cgccggcctc gaagatcatt tcatgggcaa
      661 gctcctcggc ctgccgatgg gctgcgacgt ctgttacaca aatcacgccg ccgccgatca
      721 aaattccgcg gacaatctgc ttttgcttct cggcgcggcc ggctgcaact acttcatggg
      781 cgttccctgc gcggacgatg tgatgctgaa ttaccagtcc accagctacc acgacgcgct
      841 cgccgtccgc aggctcttta agctcaggcc cgctcccgaa tttctggttt ggctcgaatc
      901 aaacgggatc tatcgcgacg cggagcctgc tttgctcgac gcgcccgcgc gccgccaatt
      961 gatgcacggt ctggaatcat cgctcgcgga gatcgcctga atgagcgaca aaccggattc
     1021 ctcgctggct gcctcggact tgcccgaaat tctccgcaag attcgcgcgc gcactcccgc
     1081 tcgcattcta atgggacgtg ccggcgcgtc ctaccgcacc agcacactga tggatttacg
     1141 cgaggcccac gcagccgcgc gcgacgccgt ccgcgcggaa ttcgatctgc aaaaagtatt
     1201 cggcgccgct ttcgcgcggc aatggcaact ctttgagaca cgcacgcggg caactagcaa
     1261 agaagaatat ttgttgcgcc ctgacctcgg caggcatttt gaacctgcat cacaagacga
     1321 aattcgtaag cgcggctcgc gggaaagcga tcttcaaatc gcaatcggcg acggcctttc
     1381 cgttcccgcc gtggtcgcgc aagttcctgc gcttttgccg ctgctgcacg agggtgccac
     1441 ggcgcgcggc tggaaaatag gccgcacgtt cgtcatccat cactgccgcg tgggaatcct
     1501 caacgaaatc ggcgaattgc tgaatccccg cgtcgccgtg ctgctgatcg gcgaacgccc
     1561 aggcttagcc acagcggaaa gtctgtcggc atacgtggcg tatcgccccc gcgcgactca
     1621 ttcggacgct gaccgaaacc tgatctcaaa tatccacgct tgcggtctga atccgcacaa
     1681 cgcagccacc cgcattctaa attttgctgc acagatgata caggccggct cgagcggctt
     1741 cactttgcga gaaaacctta ccgcgctcga caacaataaa tagccggcat ggatgaagta
     1801 acctgcttga aaggggaatt ggatcagggc gcgctattac acgtcaaact tcgaccagcg
     1861 tggccgaaag ccgcagttgc ccgttggcga cagctgcttc caaagcagcg accaccttcg
     1921 gatcaaattt ggccacagcc agcgatcgga tgcggcccag cgcatattcc aggtccattg
     1981 cggactgata tggccggttg gtggtaatcg cgtcgagcgt gtcggctacg gcgatgatgc
     2041 gtgccatcat cggaatctgg tcgccttgca ggcca
//
LOCUS       DATNDT010000066         3604 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4207565, whole genome shotgun sequence.
ACCESSION   DATNDT010000066 DATNDT010000000
VERSION     DATNDT010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3604
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4207565"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VN788_01975"
     CDS             complement(<1..327)
                     /locus_tag="VN788_01975"
                     /inference="COORDINATES: protein motif:HMM:NF012576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXU19331.1"
                     /translation="MPVVMEDVAARAGVSATTVSHVLNETRTVAPQTRRRVLEAVREL
                     NYYTNASARLLVRGRADTFGLIISDIENPFFPELIKSFERACLERRMGVLLCTTNYDP
                     EQARNGV"
     gene            complement(346..1644)
                     /locus_tag="VN788_01980"
     CDS             complement(346..1644)
                     /locus_tag="VN788_01980"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU19332.1"
                     /translation="MSDFPTRRQFVWSSAGAALLAASPARAAGANGRINVGMIGLGGR
                     GSLLLEMVLHRATEQRDIQLVALCDVYQKRLNHAKGVAQSKGQDPKTYAHHQDLLARN
                     DLDAIVVATPDQWHAPLTMLAMERGLDVYCEKPMTHTLEEAKQVAHKAKELKRVVQIG
                     VQGTSWDRWHKVREAVHSGMLGKVVACQGTYSRNDPKGDWNWPIDAGAGPDATGDLHI
                     DWKQWLGPTPQRPFDADRFFRFRKYWDYSGGIATDLHYHIVAPFHIALENEHPTRVTG
                     MGGLWVYNDGREVPDTFSNASDFPGKYNITVISSQVNEIGPLLLLRGTEATLHLGDEW
                     EGPQGRDSDVGRIVPETPFKDDFKKKYGKDEIVLTGVGNQGDQKHMDNFFDCVKSRQQ
                     PNCHADLGYKVMTTIALSVRSYREGKMFYFDPEREEAVER"
     gene            1841..2497
                     /locus_tag="VN788_01985"
     CDS             1841..2497
                     /locus_tag="VN788_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HXU19333.1"
                     /translation="MIKRILLITLLSCFAAISALCADWRPLFNGKDLTGWQHVGPGGD
                     TVENGLIHTHGGMGLLYWKGGKIGHCTLRVVYKMRDKNDNSGVYIRIPIEPREAWMPV
                     HYGYEVQIDNHPETSHEDEYHVTGTLYSLTKPLAKPGKPGPEWNTMEITIDGPHTLVS
                     VNGVKVTDYTEGQPVPARKFDFEPLRGPRPDTGYFGLQNHSNNDIVFFKEVSIKPLGR
                     "
     gene            2712..>3604
                     /locus_tag="VN788_01990"
     CDS             2712..>3604
                     /locus_tag="VN788_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXU19334.1"
                     /translation="MPKRALITGITGQDGSYLAELLLQKGYEVHGMVRRVALEDPAHR
                     LSRVAHLAGNVVFHAGSLESFPSVYTLLAKIQPDECYHLAAQSFVSYSFEDEFSTLNT
                     NINGTHFVLAALKELVPKCRFYFAGSSEMFGKVAEIPQTENTRFHPRSAYGISKVAGF
                     HMTRNYREAYNMFASSGILFNHESPRRGFEFVTRKITSGIANIAAGKQDKLPLGNLDA
                     RRDWGHARDYVDAMWRMLQRDQPDDYVVATGETHSVKEFVELAFAHVGLDYRKHVVVD
                     PQFYRPAEVDLLIGNPEKARA"
BASE COUNT          757 a         1057 c          990 g          800 t
ORIGIN      
        1 aacgccgttg cgcgcctgct ccggatcgta attggtggtg caaagcagca cccccatgcg
       61 gcgttccagg caagcccgtt cgaagctctt gatcaactcg gggaagaatg gattttcgat
      121 gtcggagatg atcaatccga acgtgtcggc gcgtccgcgc accagcagtc gtgccgatgc
      181 gttcgtgtaa tagttcagct cgcgcaccgc ctcgagtaca cggcggcgcg tttgcggcgc
      241 aaccgttcgc gtctcattca gtacatgaga tacggtggtc gcggaaacgc ccgcccgcgc
      301 ggctacatcc tccattacaa ctggcataat cgcggtaaac gcgcttcagc gctccactgc
      361 ttcttcccgt tcaggatcga aataaaacat cttgccctcg cgataggacc tcaccgatag
      421 cgcgatcgtc gtcatcacct tgtagcccag atcggcgtgg cagttcggct gttggcgcga
      481 tttcacgcaa tcgaagaagt tatccatgtg tttctgatcg ccctgattgc cgactccggt
      541 cagcacgatc tcatctttcc catatttctt cttgaagtcg tctttgaacg gtgtctcggg
      601 cacgatgcgg ccgacgtcgg aatcgcgtcc ctgtggccct tcccattcgt ccccaagatg
      661 caatgtggct tctgttccgc gcagtagcag cagcggaccg atctcattca cctgcgacga
      721 gatgacggtg atgttgtact tgcccggaaa atccgaggcg ttcgaaaaag tatcgggaac
      781 ttcgcgcccg tcgttataaa cccataaacc tcccataccg gtgactcgcg tggggtgctc
      841 gttctccagc gcgatgtgga acggcgccac gatatggtaa tgcaggtcgg tcgcaatgcc
      901 gcccgagtaa tcccagtact tacggaatcg aaagaaacgg tctgcgtcga aaggccgctg
      961 tggagtcggc cccagccact gcttccagtc gatgtgcaga tctcccgtcg cgtccggccc
     1021 tgcgccggca tcgataggcc agttccaatc gcccttcgga tcgttgcggc tatacgtacc
     1081 ctggcaagcc accactttgc ccagcattcc ggaatgaacc gcttcccgaa ccttgtgcca
     1141 gcgatcccag gatgtgcctt gcacgccgat ttgcacgacg cgcttcagtt cctttgcttt
     1201 atgcgcgacc tgtttcgcct cctcaagcgt atgcgtcatg ggtttctcgc agtacacgtc
     1261 gagaccgcgc tccatggcca acatcgtcag cggagcgtgc cactgatccg gcgtcgcgac
     1321 gacgattgcg tcaaggtcgt tacgcgcgag aaggtcctga tgatgcgcat aagtcttcgg
     1381 gtcctgaccc ttcgattgcg ccactccctt agcgtgattc agacgctttt ggtagacgtc
     1441 gcacaaagcg actaattgaa tgtcgcgttg ctccgtggca cggtgcagga ccatctccag
     1501 taacagcgag ccgcggccgc caaggccgat cattccaacg ttgatgcgtc catttgcgcc
     1561 ggcagcccgc gccgggctgg ccgcaagcaa tgccgctcca gcggagctcc agacaaactg
     1621 acggcgagtg ggaaaatctg acatggcgaa tcctttcagg ggcggcaatt tcggtcggac
     1681 gcaagcgctt gccgcaagcg aatgcagttt cgattatagt tccagaaaca aagcctgcca
     1741 agcttttatt gacatgcgcg attgctttgc tatatatttt gaagccggga cgcatacgct
     1801 tgcggcaaag gtttgcgcgg cggcttgcca tcttaacctc atgatcaaac gaatccttct
     1861 gattacattg ctttcttgtt ttgccgccat ctcggccctc tgcgccgatt ggcgtccgct
     1921 gttcaacggc aaggacctca ccggctggca acatgttggg ccgggcggcg atacggtgga
     1981 aaatggtctg attcatacgc acggcggaat ggggttgctc tattggaagg gcggcaagat
     2041 cggccactgc actcttcgag tcgtgtacaa aatgcgcgac aagaacgaca attccggcgt
     2101 gtacattcgt attccgattg agccgcgcga agcttggatg ccggttcact atggttacga
     2161 agtgcagatt gacaatcacc ctgaaacatc ccatgaagac gaatatcacg tcaccggaac
     2221 gctttactcg ctgactaagc cactcgcgaa accaggaaag cccggtccgg agtggaacac
     2281 gatggagatc accatcgatg ggccgcatac gctcgtctcc gtcaatggcg tgaaggtcac
     2341 cgactacacg gaaggccagc cggtccccgc gcgaaagttt gatttcgagc cgctgcgtgg
     2401 cccgcgcccc gacaccgggt acttcggcct tcaaaaccac agcaataatg acatcgtctt
     2461 ttttaaagaa gtatcgatca agcccttggg ccgttaactg ccctcagcct cacgtagtaa
     2521 atgaaacgcc ggcggattta accgaagtct ctcatgaccg cccacgttgg acgtgttttt
     2581 gtcgattgtg gccgagcgga aaatcatcct ccatgtgaaa aattcgttgg ccctcctgta
     2641 atcgctccgg caccttcggc gcgctgatcg aatacgcaac atgcgcctgt ggtaaaaaag
     2701 gatacaaaca gatgccgaaa cgcgctttaa ttacggggat tactggccag gacggatcct
     2761 acctggcgga gttgctgctg caaaaaggat atgaggttca cggcatggtt cgccgcgttg
     2821 cgctggagga ccccgcgcat cgcctgtccc gcgtcgctca ccttgccggc aatgtcgttt
     2881 tccacgccgg ctctcttgag agctttccca gcgtctacac tctgctcgcg aagattcaac
     2941 ccgacgaatg ctaccacctg gccgcgcaga gtttcgtcag ctattcgttt gaggacgagt
     3001 tttccacctt gaatacgaat atcaacggga cgcattttgt cctcgcggca ttgaaagaac
     3061 tggtcccgaa gtgccgcttc tacttcgccg gatcgagtga aatgttcggt aaggtcgcgg
     3121 aaatcccaca gaccgaaaat actcgctttc atccgcgttc cgcatacggc atcagtaagg
     3181 ttgcgggttt tcacatgacg cggaattatc gcgaagcgta taacatgttc gcgtcgagcg
     3241 ggattctgtt taatcacgag tcgccgcggc gcggcttcga gttcgtcacg cgtaaaatca
     3301 cttccggaat cgcgaacatc gccgccggga agcaggacaa gcttccgctc gggaatctcg
     3361 acgcgaggcg cgattggggg cacgcgcgcg attacgtcga cgcaatgtgg cggatgctcc
     3421 agcgcgatca gcctgacgat tacgtggtcg caaccggaga gactcactca gtgaaggaat
     3481 ttgtagagtt ggcgttcgcc catgtggggc tcgactatcg caaacatgtg gtcgtcgatc
     3541 cccagttcta tcgcccggcc gaagtggatc tgttgatcgg gaacccggaa aaggctcgcg
     3601 ccga
//
LOCUS       DATNDT010000067         3525 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1702234, whole genome shotgun sequence.
ACCESSION   DATNDT010000067 DATNDT010000000
VERSION     DATNDT010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1702234"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VN788_01995"
     CDS             complement(<1..604)
                     /locus_tag="VN788_01995"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HXU19335.1"
                     /translation="MVRFGTWAIAIVLGANISASANAVRHALEARYQHARTLKAEFYE
                     RYSDGSGGVTAESGVVYFSRPGRMRWEYESPEQKLFLVDGKNAWFYVPADHTASRAKI
                     KESSDWRTPLALLAGKTDLSKLCRQIELAGDAPSSKDSDLRPVAGDAVLRCVPRGSEE
                     EGSAAPDVLLEVDPQGWLARIIIHEAGNAQTEFRFGNWADN"
     gene            complement(741..2186)
                     /locus_tag="VN788_02000"
     CDS             complement(741..2186)
                     /locus_tag="VN788_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19336.1"
                     /translation="MSSTESVGTPAGVPSLSSRATWLLIALFILTIPFTSARVRGDGI
                     GYYAYARSLIVDHNLQFKGDWKGAGSEPAVVVRDSHGLSVNSHITKTGHIANYFAVGP
                     ALLWSPFIAATHAGILLLRHMGYSIAADGHGHAYLLAMAFATALYGFIGLLLSFQLAR
                     AFTGEKWALLAAVTIWLASSLPVYMYVDPSWAHAPSAFAVAWFLWYWHRTNAHRTRVQ
                     WMILGLISGLMCDIYFANAVFLLVPILDFISEGRTTFHENASWLGTLKRALQSLFIYF
                     GALLIGFLPTLAVRQIIFGNPLAAAAYASRPWQWESPKFWSILFSSSRGLFVCTPVLT
                     LAVAGLFFLRRKQPALGNRLLACTAAFYALISVYPWWDGVASFGNRFFISLTPVFVVG
                     LAVTFEKFTNVWRDQRRATRRVVAVAAVLIVWNLGLVYQWSNGLFPNVGPVYWREVVY
                     NQIRVVPGDLLRNMGSLAAHVSNDSARSADE"
     gene            complement(2275..2841)
                     /locus_tag="VN788_02005"
     CDS             complement(2275..2841)
                     /locus_tag="VN788_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXU19337.1"
                     /translation="MTSMKYKNPIRAKREGHGENSGFSLIELLIVVAIIVIIAAIAIP
                     NLLRARMAANETSAAENIRTITTASVIYNTTYGNGFPPDLQTLGGPGPTATCDSAVLL
                     DQILSASPAVKSGYTFEYTGGDGDALLGAGCTKKGFNGYLVTATPLVKQVTGQRSFCA
                     NEPAAIYFDAAGKTAPSENACVALPVLQ"
     gene            complement(2913..3467)
                     /locus_tag="VN788_02010"
     CDS             complement(2913..3467)
                     /locus_tag="VN788_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXU19338.1"
                     /translation="MNKKQKGFSLIELLIVVAIILIIAAIAIPNLLRARMAANETSAV
                     GSLRTMVTAEVSYNSTYSIGFTNDLGTLGGAAGDTVASCTEALQIDSILSNNGTGNTS
                     QKSGYQFTFTAGTVDFTPASAVPTGCTAGKVDGFAIQADALNQGTTGQRHFCTDASGV
                     TRYDGTNAITVANTTCASTAVPLS"
BASE COUNT          827 a          996 c          997 g          705 t
ORIGIN      
        1 aattgtccgc ccagtttccg aaccgaaatt ctgtttgagc attgccggcc tcgtgaataa
       61 taatccgcgc aagccaccct tgagggtcca cttccaaaag cacgtccgga gccgcagatc
      121 cttcttcttc cgaacctcgg ggcacgcaac gcagcacggc atcgcctgcc accggtcgca
      181 ggtccgagtc cttgctggat ggggcatcgc cagccagttc aatctggcgg cagagcttgg
      241 acaaatcggt tttgccggca agcagcgcca gcggcgtgcg ccaatcggag ctttccttga
      301 tcttggcgcg gctggccgtg tgatcggcgg ggacgtagaa ccacgcattc ttgccatcca
      361 cgagaaaaag cttctgttcc ggtgattcgt actcccagcg cattcgtccc ggccgcgaaa
      421 aatagacgac tcccgattcc gccgtcacgc cgccgctacc gtccgagtag cgctcgtaga
      481 attccgcctt gagcgtccgc gcgtgctggt atcgcgcttc cagtgcatgg cgaactgcat
      541 ttgcgctggc tgaaatattc gcgcctaaca cgatggcgat agcccaggtt ccaaatcgca
      601 ccatagaact tcgagtttaa cgtactgccc gagcgcccct gtttatcaat ttagatttgg
      661 gtgttcggac ggccatcaaa ctggcttctc taaaagacat gttcgaaatg agaactctgt
      721 actgaagaag accaacaatt tcattcatct gcgcttcggg cggagtcatt ggaaacatgt
      781 gcagccagag agcccatgtt ccggagcaaa tctcccggca ctacgcgaat ctggttgtaa
      841 accacttcac gccaatacac aggaccaaca tttgggaaaa ggccgttact ccattggtag
      901 accaggccga gattccagac gatcagcacc gctgcgaccg caaccacgcg tcgcgtcgcg
      961 cgacgctgat ctcgccagac gttggtgaac ttttcgaaag tgaccgcgag acccacgaca
     1021 aaaactggcg tcagcgagat aaagaagcga ttcccgaagg acgccacccc atcccaccac
     1081 ggataaactg aaatcagcgc atagaaggca gcagtgcatg cgagaagccg gttccccaac
     1141 gcaggttgct tccggcgcaa aaagaatagg ccggcgaccg caagtgtgag aacaggggtg
     1201 cacacgaaca ggccgcggct ggaagaaaaa agaatgctcc aaaacttcgg cgattcccat
     1261 tgccaaggcc gtgaagcata ggcagccgcc gcaagcgggt tcccaaagat tatttggcgc
     1321 accgccaacg tgggtaggaa tccgataagg agcgctccga aataaatgaa caagctttgc
     1381 agcgcgcgtt ttagggtgcc gagccatgac gcattctcgt ggaaggtcgt tcggccctca
     1441 gagatgaaat cgaggatggg cacgaggaga aatacggcat tagcgaaata gatgtcgcac
     1501 attagccccg atatcaggcc caaaatcatc cactggacgc gagtgcgatg agcgttcgtg
     1561 cgatgccaat accatagaaa ccaagccacc gcgaatgcgg agggggcgtg agcccatgag
     1621 ggatcgacgt acatataaac cggcagggaa cttgccagcc atatcgtaac agcggccaaa
     1681 agcgcccatt tctctccggt aaaggctcga gctagctgaa aggaaagcag cagaccgata
     1741 aatccataca gagcggttgc gaacgccatg gccaaaaggt aggcgtggcc atggccatcc
     1801 gcggcaattg agtaccccat gtggcgcagt agcaggattc cagcgtgggt agctgcgata
     1861 aagggcgacc agagtaaagc cggaccgaca gcgaaataat tcgcaatgtg gccggtcttc
     1921 gtgatgtggc tgttgactga aaggccgtga ctatcgcgca ccactacggc cggctcagaa
     1981 ccggcgcctt tccaatcgcc cttgaattgg aggttgtgat cgacaatcag cgaacgagcg
     2041 tacgcataat agccaatccc atcaccccga actcgagcgc ttgtgaatgg aatggtcagg
     2101 atgaacagcg cgataagaag ccacgtggcc cgcgaagaga gggaaggcac gcccgccggc
     2161 gtaccgactg attcggtaga ggacattgtt tcttgcccaa gatactgtaa gtgacgggat
     2221 ccgttgtggg tgtcgcgcta caaactcggc acgatcgcca caactcgcca acggctattg
     2281 taaaacgggg agggcaacac aggcgttctc ggacggcgct gtttttccag cagcgtcgaa
     2341 gtaaatcgcg gctggctcgt tcgcgcaaaa gcttcgctgt cctgtcactt gcttgacaag
     2401 cggagttgca gttaccaggt agccgttaaa tcccttctta gtgcagccag cccccagcag
     2461 cgcgtcgccg tcgccccccg tatattcgaa cgtgtaacca ctcttcacgg ctggagaagc
     2521 actaaggatc tggtcaagaa ggaccgcgct atcgcaggtt gccgtcggtc ccggcccacc
     2581 gagagtctgt agatcggggg gaaagccatt cccgtacgtt gtgttgtaga tgaccgatgc
     2641 cgtggtgatt gtgcggatgt tctcggctgc agaggtttcg tttgccgcca tgcgcgctcg
     2701 gagaagattc gggatggcga tggcagcgat gatcacgatg atggctacga cgatcaggag
     2761 ctcgatgaga gaaaagcccg aattctcgcc gtgcccctcc cgttttgccc gaattggatt
     2821 cttgtatttc atcgaagtca ttcagagcac tttcacggca aagaaaaagg ggaggaagct
     2881 tgctccctcc cctctatttt cacttaccct tactacgaca gcggaacagc ggtcgaggcg
     2941 caggtcgtgt tcgctacggt gatcgcgttc gtcccgtcat agcgagtcac gccagacgca
     3001 tcggtgcaga agtggcgctg acccgttgtg ccctgattca gcgcgtctgc ctggatggcg
     3061 aaaccatcca ccttacctgc cgtgcaacct gtcggtaccg ccgacgccgg cgtaaagtct
     3121 accgtgcccg cggtgaaggt gaactggtag ccgctctttt ggctcgtatt tccagttcca
     3181 ttgttcgaaa gaatggagtc aatctgaagt gcctctgtgc aagacgcgac agtatcgccg
     3241 gccgcgccgc cgagggtacc cagatcgttc gtgaagccga tgctatacgt ggagttatag
     3301 gaaacctccg ccgtaaccat cgtgcgtagt gagcccacag ccgatgtttc attggccgcc
     3361 atacgggcgc gaagcaggtt cggaattgcg atggccgcga tgatcaggat gatcgccacg
     3421 acgatcagca actcgattaa cgagaagccc ttctgtttct tgttcattgt ctctcctagg
     3481 agcagcgctc cggaatctct agttgtacgc cctggcgggg tacgc
//
LOCUS       DATNDT010000068         6226 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_478033, whole genome shotgun sequence.
ACCESSION   DATNDT010000068 DATNDT010000000
VERSION     DATNDT010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6226
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_478033"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="VN788_02015"
     CDS             <1..174
                     /locus_tag="VN788_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU19339.1"
                     /translation="NPQKVLLKISQEMLAEMVGTTRSRVNFFMNKFRKLGFIQYNGEI
                     QIDDSLLSVVLHE"
     gene            460..2064
                     /locus_tag="VN788_02020"
     CDS             460..2064
                     /locus_tag="VN788_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXU19340.1"
                     /translation="MRVLLYNPDNGVTRNFMPHLWMFLLQALTPPGHEVVLIDGNAQP
                     MDEESIVRYVRENSIELVGIGAMTRMIAKAYRIADAVRAAGVKVVMGGPHVTEMADEA
                     LGRDGGSRHADAVALGEADETWPRIVQDAARGTLKDVYAPVDDFGQERKPSLQPYPKI
                     PWDRINLDQFNLLPGMLHPLLKRVGGGWGTFRIIPMESGRGCPYGCEFCTVTGFFGDA
                     IRFRTNESVVNELLLLKARARKEGGQIAVFFIDDNFAINIKRTKSLLRDIIAANAQVH
                     WVAQISANLLRDEELVDLIAASGGKWIFIGMESIDPSNLASVNKGFNKPGEYATVLER
                     LAKRNIYAITSFIFGMDNDTPGVAERTLHEIATWPPGLPIFGLLTPLPSTPLYKRLEA
                     AGRLTRPKHWQEFIPFAMAHTPLKMSIEEAHREVRIGWANSYSPQAIERAVDSLNDKL
                     LGYRINILIGRLCFRGIYFPQMGRFAWMKVMAGNRRTIFKLVKKAFKERLRTNRTSKR
                     PSPTLCSQSSLPSEGSGRNSKSFAGK"
     gene            2597..3586
                     /locus_tag="VN788_02025"
     CDS             2597..3586
                     /locus_tag="VN788_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GRP family sugar transporter"
                     /protein_id="HXU19341.1"
                     /translation="MFIPHTYWTALILMLVSMLCWGSWANTQKIDKRWRFELFYWDYV
                     WGLLACALAFGLTLGGRNSASPDSFFRNLNAASVRSLLEAFFGGVIFNVGNLLLVAAI
                     SVAGMAVAFPIGAGLALVIGAVINYVISPKGNPILLFGGIALICVAILLDAMAYRGLP
                     GGAKATTKGVGLSLACGVAVGLFYPFVAKALTGPNHLGPYMVFFVFVLGALVSNFPMN
                     YALMRRPVSGPPLQMRDYFNGHVSLHAWGILGGAIWGIGTIANFAASYVPMIGPATSF
                     SLGEGNTMISAIWGIFVWREFHGAGKRVKGLLAIMFVFFILGLSCIALAPVFR"
     gene            3626..6025
                     /locus_tag="VN788_02030"
     CDS             3626..6025
                     /locus_tag="VN788_02030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013334697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXU19342.1"
                     /translation="MTKLDNENGAFGSPGIHPRWTHGGKDGVGTAYAASSQIWFTLWN
                     GIITEVYYPTVDRPQLRDLQYLITDGTSFFHEEKRHLKSKFERLSDHALGYRCTNSDP
                     EGRYAVVKEIITSPHLACVLQRTKLTGDEPFVSNLRLYALCAPHLEVGGWGNNGYVAE
                     LSGRKILMAQKQGTWLALAATIPFSRVSCGYVGHSDGWTDLAGNFQMDWEFGHASDGN
                     VALTGQLDVSAHREFTLGLAFGDTQHHAIATLFQALGTPFEEHHQRYTEQWNRTAAHQ
                     FPLEKYSSDQGNLYHSSFSLLLAHEDKSYPGALIASLAIPWGEARGDKDQGGYHLVWT
                     RDMVNSASALLACGESGTPLRALIYLAVSQQEDGGFSQNFWVNGNAYWRGIQLDEVAF
                     PILLALRLCQQNALQDFDPYPMVLKAASYLLRHGPVTQQERWEEASGYSPSTLASNIA
                     ALIGAAYFCRQRGDKTAAEFLEQYADFLESHVDTWTVTTEGTLVPGIRRHYIRILPAD
                     VDNSTPAEDPNSGILKIANLAPGANNSFPAKEIVDAGFLELVRYGIRQPHDPVIVDSL
                     KVVDAVLKVNTRVGPVWHRYNHDGYGQREDGGPFKGWGKGGAWPLLTGERAHFELAAG
                     QDIKPLIRAIEGFASATGLLPEQVWDEQDKPERHMFLGRPTGSAMPLMWAHAEYIKLL
                     RSASDGRVFDLIPEVAHRYLGDRKDCQRFEIWKPNRRVRVVKKGYTLRFQVPAPFRLH
                     WTGDEWRTVNDTASARTTFGIEFVDLPITTSQRAPIRFTFFWPENNSWEGRDYMVEVE
                     H"
     gene            complement(6044..>6226)
                     /locus_tag="VN788_02035"
     CDS             complement(6044..>6226)
                     /locus_tag="VN788_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19343.1"
                     /translation="DVESDAKLNDQILAEIPMGRWGKPEEVAQLAVYLASDEAAYVTG
                     STHFIDGGMLRNAGRL"
BASE COUNT         1360 a         1685 c         1771 g         1410 t
ORIGIN      
        1 aatcctcaaa aggtactcct caaaatatcg caggagatgc tggcggagat ggttggcacg
       61 acgcgttccc gtgtcaattt cttcatgaac aaattcagga aactgggctt tattcaatac
      121 aacggcgaga tccaaatcga cgattcccta ttgagcgtcg tcctgcacga gtaaggagcc
      181 gtttccgagg gaacgaatct cgtaatcaac tatatggaag caatgaatta catgaaaggc
      241 aatcttcgat ctgactctga ccttagtcgt gtttatggtg ggtattactg cttgcgattc
      301 ggacgcgtcg cgcaacgaag tctcgtcacg aggaagttta agtgtccttg tcatcgagga
      361 ggaccatcct cgtcaatgaa ggacagtttc tcgccgttgc tagttacaag tgctgctagg
      421 ccggatcact agcaggtgct ttcctgccga ggaagttcca tgcgcgtttt actttacaat
      481 cccgacaacg gtgtgacacg caacttcatg ccgcatcttt ggatgttcct tctgcaggct
      541 ctgactccgc ctggccatga agtggtcctg attgatggca acgcccagcc catggacgaa
      601 gaaagcattg tgcgctacgt ccgtgaaaac agtatcgaac tggttggcat cggcgcgatg
      661 acccggatga tcgcaaaggc ttatcgcatc gcggacgcgg ttcgcgctgc aggagtgaag
      721 gtcgtgatgg gaggcccaca cgtaactgaa atggcagatg aggcgctcgg gcgcgacggc
      781 ggctcgcgtc acgccgacgc ggttgcgttg ggcgaagccg atgagacgtg gccacgaatc
      841 gtccaagacg ccgcacgcgg tacactcaag gacgtttacg cgccggtgga cgacttcggc
      901 caggagcgca aaccgtcact gcagccgtac ccgaagatcc cttgggacag aatcaacctg
      961 gaccagttca acttactgcc gggcatgttg catccgttgt tgaaacgggt gggcggcggc
     1021 tggggaacgt ttcggatcat tcctatggag tccgggcgcg gatgccctta cggatgcgag
     1081 ttctgcacgg tgacgggatt ctttggcgac gccatccgct ttcgcacgaa tgagagcgtg
     1141 gtcaacgaac tcttgttgct caaggcgcgc gcgagaaaag agggaggcca gatcgctgta
     1201 tttttcatcg acgacaattt tgccatcaac ataaagcgca caaaatcgtt gttgcgcgac
     1261 attatcgccg ccaacgcaca ggtgcattgg gtggcccaga tcagtgcgaa tttgctgcgc
     1321 gacgaggaac tcgttgatct cattgcggcc tctgggggta aatggatttt tattgggatg
     1381 gaatccattg acccgtcgaa tctggccagc gtaaacaaag gattcaacaa gcccggcgaa
     1441 tatgcgactg ttctggaaag actggcgaag cgcaatattt atgccatcac ctcttttatt
     1501 ttcggcatgg acaacgatac gcccggagtc gcggagcgca cccttcacga gattgcgacc
     1561 tggccgccag gattacccat tttcggttta ctcacgccat taccttcgac accgctctat
     1621 aagagactgg aagcggctgg acggctgacg cgtcctaagc actggcagga gttcatacca
     1681 tttgcgatgg cgcatacgcc gctgaaaatg agcatcgagg aggctcatag ggaagtgcga
     1741 atcggatggg ctaactcata cagcccgcag gcaatcgaga gggccgttga ctctctcaac
     1801 gacaagttac tgggttatcg catcaacatc ctgatcgggc gtctctgttt tcgggggatt
     1861 tattttccgc aaatgggccg gttcgcgtgg atgaaagtaa tggccgggaa tcgccgtacg
     1921 attttcaagc tcgttaagaa agcattcaag gagcgattgc gaacaaaccg cacctctaaa
     1981 cgcccttccc ccaccctctg ttcacaatcc tcgctcccat ccgaaggctc tggtcgcaac
     2041 agcaagtcgt tcgctggaaa atgatccgat cagtgccgat gcaatgttct tttggacggg
     2101 ttatgctgtg gtacgtatca attccacgct gaacggcaga accaaccaat tcggatggtt
     2161 tgcaggcccc tgcaaccgaa cttcttcttc ttcgcaaaga agttcggcgg aaccgtgcga
     2221 ccatccgaat ttgtgattct cgttgttagg gcgtatctgg acttcgtttc ccactccgcg
     2281 ttcctcgatg gatatacctg gcagataatt tcctcgtcct tctttattct ttcctgcaga
     2341 tcgtccacaa tcgcacagct ttggatgtcg cagagcgtga tactcgctcc cggaacagcg
     2401 aaaagaaggc gctcaggatc ggtgcgatcc acaaaatgtg cgtcagcgcg actactgcta
     2461 cgcgaaattg gcggatttgc ttcagggagc taaagctaaa tgaaaatccc gggggaccga
     2521 ctcaagaatt gagcggagcg tctactttga gccttcttgt ccgtgatcta ttcgcccagc
     2581 cgtggccgag gtgagattgt ttatcccgca cacctactgg actgctctga tcctcatgct
     2641 ggtcagcatg ctgtgttggg gaagctgggc gaacacgcag aagattgaca aacgctggcg
     2701 gttcgaactt ttctactggg attatgtctg gggccttctg gcatgcgcgc tggcgttcgg
     2761 attgaccctc gggggtagga actccgcgtc gccggacagc ttttttcgga atctaaacgc
     2821 cgctagcgtt cggagtcttc tggaggcttt cttcggcggc gtaatcttca acgtcggaaa
     2881 ccttcttctg gtggctgcga tatcagtggc cggcatggcc gtggcgttcc cgataggagc
     2941 aggccttgca ttggtgatcg gagccgtcat caactacgtc atttccccga aggggaatcc
     3001 cattctcctt ttcggcggca tcgcactgat ttgtgttgcc atcctgctcg atgcgatggc
     3061 ctatcgcgga ctgcccggag gcgcgaaggc cacgacaaaa ggggttggat tgagtctggc
     3121 ctgcggcgtt gccgtcggcc tgttctaccc gttcgtcgcc aaggcgctaa caggcccgaa
     3181 tcacctgggg ccctatatgg tgtttttcgt ttttgtcctg ggcgcgctcg tatccaattt
     3241 cccaatgaat tacgcgttga tgcgccggcc agtgagcggc cctcccttgc aaatgagaga
     3301 ctatttcaat ggacatgtga gccttcacgc gtggggaata ttaggcgggg cgatttgggg
     3361 catcggtacg attgccaact ttgcggcttc ctacgtgccg atgattggac ccgccacctc
     3421 attctcgctt ggcgaaggca acaccatgat ctccgccatc tggggaattt ttgtatggag
     3481 ggagtttcat ggtgctggaa agcgagtcaa gggtctcctc gcgattatgt tcgtattctt
     3541 tattttggga ttgagttgta tagccctagc tccggtattc cgatagaaag ccgaaggcgg
     3601 acaaagacag gaggggagaa gtaagatgac caagttggac aatgagaacg gagcgtttgg
     3661 aagccctgga attcatccca gatggaccca tggtggcaaa gacggagtcg gaaccgcata
     3721 cgcggcttcc agccaaatct ggtttacgct ttggaatggc atcatcaccg aagtctatta
     3781 cccaaccgtg gaccggccgc aactgcgcga cctgcaatac ctgattaccg atggaaccag
     3841 cttctttcac gaggagaagc gccacctcaa atccaaattc gagcgcttat ccgatcacgc
     3901 gctcggctat cgctgcacga actccgatcc cgaaggccgc tacgccgtgg taaaggaaat
     3961 tatcacaagc cctcatcttg cctgcgttct ccagcgcacg aaattgacgg gcgacgaacc
     4021 gttcgtgtcc aatctgcggc tgtatgccct gtgcgctcct cacctggaag tcggaggatg
     4081 ggggaacaat ggatatgtcg ccgaattaag cggccgcaag attcttatgg cgcagaaaca
     4141 aggaacgtgg ctggccctgg cagcgaccat tccgttttcc cgcgtgtcgt gtgggtatgt
     4201 cggccatagc gacggctgga ctgatttagc gggcaatttt cagatggact gggaattcgg
     4261 ccacgcgagt gacgggaatg tagcactcac tggacaactg gatgtgagtg cccaccggga
     4321 gttcacgctg ggccttgctt tcggcgatac acaacaccac gcgattgcga cactatttca
     4381 ggcgctgggc actccctttg aagagcatca ccaacgctac accgagcaat ggaaccgcac
     4441 ggccgctcac cagtttccgt tggagaagta ttcgagtgac cagggcaatc tctatcacag
     4501 cagtttcagt ctgcttctgg cgcacgagga caagtcctat ccgggggcat taattgcctc
     4561 tctcgcaatc ccctggggag aagcgcgagg cgacaaagat cagggggggt atcacctggt
     4621 ttggacgcga gacatggtca acagcgcctc cgctctgctc gcctgcggtg agagcggcac
     4681 cccgctgcgg gcgctcatct atctcgcggt gtctcagcag gaagatggcg gcttctcaca
     4741 gaacttctgg gtcaacggca acgcgtattg gcggggaatc caactggatg aggtcgcctt
     4801 ccccatattg ctggccttgc gcctctgcca gcaaaatgcc cttcaggatt tcgaccctta
     4861 tccgatggtc cttaaagccg cgagttactt gcttcggcac gggccggtga cgcaacagga
     4921 acgatgggag gaagctagcg gttattctcc atcgactctg gcctccaaca tcgcggccct
     4981 gatcggcgcg gcctattttt gtcgccagcg gggggacaaa accgctgccg agttccttga
     5041 gcaatacgcc gactttctgg aatctcatgt cgacacctgg acggtcacaa cggaagggac
     5101 tctggtcccc ggcattcgac gccactacat tcggattctg ccggccgatg tggacaattc
     5161 tacgccagcg gaagacccga actcgggaat cctgaaaatc gccaaccttg cgccaggcgc
     5221 taacaactca tttccggcaa aggaaatcgt cgatgctggc tttcttgagc tggttcgtta
     5281 cgggataaga cagccgcacg atccggtgat tgtcgattca ttgaaggtgg ttgatgcagt
     5341 tctgaaagtg aatacccgcg ttggacccgt ctggcatcgt tacaaccacg atggctacgg
     5401 ccagcgggag gacggaggac ctttcaaagg atggggcaaa ggtggtgcgt ggccgcttct
     5461 gaccggagaa cgggcgcatt ttgaactcgc tgcgggacaa gatataaaac cattgatacg
     5521 ggcgattgaa ggctttgcct cggcgacggg cctcctgccg gagcaggttt gggatgagca
     5581 ggacaagccg gaaaggcata tgttccttgg ccgcccgacc ggctcggcga tgcctctgat
     5641 gtgggcgcac gcggagtaca tcaagctcct caggtccgca tccgatggcc gcgtcttcga
     5701 cctgattcct gaagtagcgc accgctacct aggagatcgc aaggattgtc agcgatttga
     5761 gatctggaag ccaaatcggc gagtgcgtgt cgtcaagaaa ggctacactc tgcgcttcca
     5821 ggttccggcc cccttccgac tgcactggac aggtgacgag tggcgcacgg tcaacgatac
     5881 agcctccgcg cggaccactt tcggaattga gttcgtcgat cttccaatca ccacatccca
     5941 gcgggcgccg attcgattca ctttcttctg gccggaaaac aattcatggg aaggccgcga
     6001 ctacatggtt gaggtggaac actaagaagg acgggttggc cagttacaga cggccggcgt
     6061 ttctcagcat accgccatcg atgaaatgcg tgctgccggt aacgtaggcg gcttcgtcgg
     6121 aagccaaata cacggcgagc tgagcgacct cttcgggctt gccccagcgc cccatgggaa
     6181 tttccgccaa aatctggtcg ttcaatttcg catcgctttc gacatc
//
LOCUS       DATNDT010000069         3884 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1334453, whole genome shotgun sequence.
ACCESSION   DATNDT010000069 DATNDT010000000
VERSION     DATNDT010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3884
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334453"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1641)
                     /gene="typA"
                     /locus_tag="VN788_02040"
     CDS             complement(<1..1641)
                     /gene="typA"
                     /locus_tag="VN788_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HXU19344.1"
                     /translation="MNTPIRNIAIIAHVDHGKTTLVDAMLRQSGIFRENQEVPRRVMD
                     SNELERERGITILAKNTAITYRGTKINIVDTPGHSDFGGEVERALRMVDGVLVLVDAS
                     EGPLPQTRYVLQKALAAKLPPIMVLNKIDRPDARPAAVLDEIYDLLIDLDATEDQLDF
                     PVIYTNAKTGVAHRKIGDDSTTLEPLFEEIVASIPEPAGDPSGVLQIQVTNLDYSDFL
                     GRLAIARVFQGTLERGTEVAISKLDGRIQPTKITKIFTFRGLERDEAETVSAGDIVAI
                     AGVEGIQIGESITDLANPSPLEPLTIDEPTLTMVFTINTSPFAGREGQFVTSRDLRAR
                     LEKELLTNVSLRVEDTGTTDAFRVLGRGELQLAILIETMRREGYELMVGKPEIVVRTE
                     NGRRLEPLELLVVDCPESFIGIVMESLGTRRGEMKKMVNHHSGRVRMEFSIPSRGLIG
                     LRSQLLTDTRGTALIHSLLEGWTEYAGDMAMRLTGALVCDRPGVAVAYAIWGIQERGE
                     MFVGPGVEVYEGMIVGENSREDDMNVNITKEKKQTNMRS"
     gene            2026..3390
                     /locus_tag="VN788_02045"
     CDS             2026..3390
                     /locus_tag="VN788_02045"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HXU19345.1"
                     /translation="MSLVRSIGRWAMAALVVNCIIGSAIYGIPSELTGLLGRASPLVM
                     VGAGLAMATIMLSFAEVASQFSDPGGPYLYVRTAFGRFAGLQVAWFWLLSVVAAAGAA
                     ANLFTSYLAGMLPSVEHGIWRTLLMTILIAVPLMANWVGARSGAALSSVLTVAKVLPL
                     ALLIVFGLIRFGPHAQLVHASEITAPGWKPWLTALLVVFFAYSGFEDAIVPAGEVKNP
                     KRAMPFAILVGLLVSMVVYTLVQFVSAAAAGAGHGDRPLADVASLLIGRGGETFVEIA
                     AMLSTYGYISATVLSAPRLACAFAAHGDGPAFLGKLHHRRHTPTLAIAIYAASVWVLA
                     LTGTFRWALELSVGASTIYFAGVCATLIRLRKLHPQVDAVRIPLGPVWAVAGIALSLL
                     LITQLNLREILLMGVTALIATANWVWAKRRHSSMRNGLKSSGAVGASSGDVAGDDRFA
                     ELRE"
     gene            complement(3506..>3884)
                     /locus_tag="VN788_02050"
     CDS             complement(3506..>3884)
                     /locus_tag="VN788_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013565853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXU19346.1"
                     /translation="AETEKLLLVKKAEAEAESKRLQGEGIANQRKAIVEGLRDSVAKF
                     SESVEGATPKDAMAMVLLTQYFDTLKEVAGTNRSNTIMMPHTPNALTDLFAQFRNAII
                     TGDIAANGADGNVAPLPTPTHRV"
BASE COUNT          679 a         1195 c         1107 g          903 t
ORIGIN      
        1 cgaccgcata ttcgtctgct ttttctcttt ggtgatgttc acgttcatat cgtcttcgcg
       61 cgaattttcg ccgacgatca ttccctcgta aacttccacg cccggcccca caaacatctc
      121 gccgcgctcc tggattcccc agatcgcgta cgcaaccgcc acgcccggcc ggtcgcacac
      181 tagcgcgccg gtaaggcgca tggccatgtc gcccgcatat tcggtccagc cttcaagaag
      241 cgaatgtatg agcgccgtgc cgcgagtatc cgtaagcagt tggctgcgca gtccgatcaa
      301 tccgcgcgaa ggaatgctga actccatgcg cacgcgtccc gagtggtgat tcaccatttt
      361 tttcatttcg ccgcgccggg tgccaagcga ctccatcaca attccgataa acgattccgg
      421 gcaatccacc accagtaatt cgagcggctc gagccgccgt ccattttcgg tgcgcaccac
      481 gatctccggc ttgcccacca tcagttcgta tccttcgcgc cgcatcgtct cgattaaaat
      541 cgcgagctgc aattcgccac ggcccagcac ccgaaacgcg tcggtggtgc cggtatcttc
      601 cacacgcagc gaaacattcg tcagcaattc cttctcgagc cgcgcgcgca aatcgcgcga
      661 agtaacgaac tgcccttcgc ggcccgcgaa cggcgaagta ttgatcgtaa acaccatcgt
      721 cagtgtcggc tcgtcgatcg tgagcggctc gagcggcgac ggattcgcga ggtcggtgat
      781 gctttctccg atctgaattc cctccactcc cgcaatcgcc acgatgtcgc ccgccgaaac
      841 tgtctccgcc tcatcgcgct ccaagccgcg gaacgtgaaa atcttggtga ttttcgtcgg
      901 ttgaattctc ccgtcgagct tcgagatcgc tacttcggtg ccgcgttcga gcgtgccttg
      961 aaacacgcgc gcaatcgcta agcgccccaa aaaatcgctg taatccaggt tcgttacctg
     1021 aatttgcagc actccggagg gatcgcctgc cggctcagga atcgacgcga cgatttcctc
     1081 aaaaagaggc tcaagcgttg tcgaatcgtc gccgattttg cggtgcgcca cgccggtttt
     1141 tgcatttgtg taaatcaccg gaaaatcgag ctggtcttcg gtggcatcca gatcgatcag
     1201 aagatcgtag atctcatcga gaacggcggc agggcgcgcg tccggccggt caattttatt
     1261 cagcaccatg atcggcggaa gttttgcggc gagcgctttt tgcagcacgt agcgcgtctg
     1321 cggcagaggc ccctcgctcg catccacgag aaccagcacg ccatccacca tgcgcagcgc
     1381 gcgctccact tcgccgccaa aatcgctgtg gcccggcgtg tccacgatgt tgattttcgt
     1441 cccgcgatac gtgatcgccg tatttttcgc cagaatcgtg atgccgcgct cgcgctccag
     1501 ttcgttcgag tccatcactc gtcgcggcac ttcctgattc tcgcgaaaaa ttccgctctg
     1561 gcgcagcatt gcgtccacga gcgtcgtctt gccatgatcc acatgggcga tgatggcgat
     1621 attgcgaatg ggcgtgttca ttgattgctc aggatcttcg aaccagacga ccgttgccaa
     1681 aagagggctg ttggttttct gccgaattga tcctctatca tacccgaaat cctaacctgg
     1741 gaccccgttc cccgattgcg cggcggttgt aggggcacgg catgccgtgc ccgcctgtcc
     1801 tgagcggtgt ggcgaagggg caaacatact tggctacgtg tccgccactc gccccgcgca
     1861 acgggcacct catgtggagt gcgcgagcgt gcccctcgca tcaaggtcca accgtggcgc
     1921 agacactcct gcctgcgcgc gtgtgttgag ttactaccca ccgccgattt ggcggttcga
     1981 gtcgatcttg atgctatact ccgcgccact tctccggagg cttgcgtgag cctcgtacgc
     2041 tccatcggac gctgggcgat ggccgccctg gtcgtcaact gcatcattgg cagcgccata
     2101 tatggaatcc cttcggagct gactggtttg ctcggcaggg ccagtccgct ggtaatggtt
     2161 ggcgccgggc tggccatggc aacgatcatg ctgtctttcg ccgaagtagc ctcccaattt
     2221 tccgatcctg gcggcccgta cctttatgtg cggacggctt tcggccgttt cgccggcctg
     2281 caagtggcat ggttctggct gctttcggtc gtggctgcgg cgggggcggc tgcaaacctc
     2341 ttcaccagct atctcgcggg catgttgccc agtgtagagc atggaatctg gcgtaccctg
     2401 ctcatgacaa ttttgattgc cgtgccactc atggccaact gggttggcgc acggtccggc
     2461 gccgcactca gcagcgtcct tactgtggca aaggttctgc cgcttgcgct gctgatcgta
     2521 tttggcctca tacgttttgg tccgcacgcg caactcgtcc atgcctcgga gatcactgcg
     2581 ccaggatgga agccatggct gacggcctta ttagtggtgt tcttcgcgta ttcgggattc
     2641 gaggatgcca tcgtgcccgc tggtgaagtg aaaaatccaa agcgtgccat gccttttgcg
     2701 attctggtgg gcttgctggt aagcatggtg gtatacaccc tcgtgcagtt cgtatccgcg
     2761 gccgccgctg gcgcagggca tggcgaccgt ccgctcgcgg atgtggcttc gttgttgatc
     2821 gggcgcggag gggaaacgtt tgtcgaaatc gcggcgatgc tttcgaccta cggatacatc
     2881 tccgcaacgg ttttgagcgc gccgcggttg gcgtgtgcgt ttgccgcgca cggagacggc
     2941 ccagcctttc tcggcaaact ccaccaccgc cgccacacgc ccacgctggc cattgctata
     3001 tacgccgcgt ccgtctgggt gctcgcgttg acgggaactt tccgctgggc gctcgagctc
     3061 tccgtgggag cgtccacgat ttatttcgcc ggcgtgtgcg ccacgctgat tcgtctgcgg
     3121 aaactgcatc cccaagtgga cgccgtgcgc attccgcttg ggcccgtttg ggccgtggcc
     3181 gggatcgccc tctcgttgct attgataact cagctcaatc tccgcgaaat tcttctgatg
     3241 ggtgtcacgg cacttatcgc cacggcgaat tgggtgtggg cgaagcggcg gcactcaagt
     3301 atgaggaacg ggctcaagag cagcggcgcc gtcggtgcct ctagcggcga tgtcgccggt
     3361 gatgatcgct tcgcggaatt gcgcgaataa gtctctaagc ctacgcccct cgcgaccggt
     3421 tacagccttg tacaaatcct cgggaattct ttcgcaggga taagtccaaa cgcggagtca
     3481 cagcgtaagc cggcatcgtg ggtggtcaga cgcggtgcgt aggcgtcggc aatggcgcta
     3541 catttccgtc ggcgccattg gcggcaatgt cgccggtgat gattgcgttg cggaattgcg
     3601 cgaaaagatc cgtcagcgcg tttggagtat gcggcatcat gatcgtgttg ctgcgatttg
     3661 ttcccgccac ttccttcaaa gtatcgaaat attgggtgag caggaccatc gccatggcgt
     3721 ccttcggtgt cgcgccttcc accgattcgg agaattttgc gacagaatcg cgcagacctt
     3781 ccacaatggc cttccgctga ttggcgattc cttcaccttg cagccttttc gattcggcct
     3841 ctgcttcagc tttttttacc agcagaagtt tttcggtctc ggct
//
LOCUS       DATNDT010000070         3765 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2357221, whole genome shotgun sequence.
ACCESSION   DATNDT010000070 DATNDT010000000
VERSION     DATNDT010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3765
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2357221"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VN788_02055"
     CDS             complement(<1..401)
                     /locus_tag="VN788_02055"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXU19347.1"
                     /translation="MKTRALAAIPVLALVLAGLVTSASARTVGETNRPVAEQVRHQLA
                     LLPWYGVFDDLQYQVNGTQVILSGNVSPLHDQTKYDAEKVVKRIPGVTQVVNDIHVLP
                     LSRFDDQIRRAEFRTIFSRPGLGRYTLGTIP"
     gene            779..1150
                     /locus_tag="VN788_02060"
     CDS             779..1150
                     /locus_tag="VN788_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19348.1"
                     /translation="MRKNANWLLGAALACASLFLAAAASGQVTLEVMNPRGEIPPPKT
                     AGISPRGANLDGKKIVLIDNGKFGASNFLAVLADLLKQKYPKATVVMYPKPAAQTITD
                     LPKWYPTVKQQGDLFVFGVGD"
     gene            1205..2584
                     /locus_tag="VN788_02065"
     CDS             1205..2584
                     /locus_tag="VN788_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXU19349.1"
                     /translation="MPGVYFTTTEFLDDAKSASKDRGMPGVRMLALPADKYYVARGNK
                     QEAQPIADAFFDKMVDALTRPLTAEEAKPSQAQKQHEDANINVTGKDFLDASGKVNEL
                     FLENRWADGLPIVPPTPELVKAMEAGTALSPQKVIGQVAPKNGTATVEKIAINAVMAG
                     AKPEYFPVILAAMAGFTDKHYDLTHVVASTGSFTPVVIVDGPITQELGFNSGIGMLGI
                     GWQANSTVGRALQLSLLNIGQTWPQVNFMGLTGRLEGYTFYTFAEDTAKSPWEPYNMS
                     QGFQKGDSTVTVSSAGNPAVFGGGAVAPWTAQGVIDQMVAQIRNRGVAWPHAQKHIVV
                     LHPDCAVELANKGYTRKSLQEYFYEHTRVPYEKLVGGLAAFGEGSEEALIRASVADGR
                     IRPDRAQIFLDNLKPGGRVPVVQSPEDFHIVVAGGSPGYDLLFTYPGTNWAHQTVKIT
                     GATPTKTGN"
     gene            2994..>3765
                     /locus_tag="VN788_02070"
     CDS             2994..>3765
                     /locus_tag="VN788_02070"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU19350.1"
                     /translation="MKKLAALAILVLSAVMLLRAQEGAPAPAAPGQGARPGARNSAQK
                     EAEAAAASRGSTIFKENCAACHGENLTGGRGPDLIRSSLVRHDKNGDLIGPVVTNGRT
                     EKGMPAFPFTQAQVADLIAFIQAQLALFDLHTRFSPYPNDIPASRLATGTVEAGKSYF
                     EAHCSSCHSPTGDLAHVASKYNPPDLEVRFLYPSGAPSTATVTLPSGDKFEGTLLLND
                     GFNVAIQGKDGWYHSWPRSDVKLEVHDPLAAHAALLQKY"
BASE COUNT          832 a         1077 c         1124 g          732 t
ORIGIN      
        1 tgcggaatcg tacccagcgt gtagcgcccg agtccaggtc gagagaaaat cgtcctgaat
       61 tccgcccggc gaatttggtc atcaaagcgc gagagcggga gaacatggat gtcgttgacg
      121 acctgtgtca ctcccgggat cctcttgacg accttttccg cgtcgtattt ggtttgatcg
      181 tgtagcgggc tcacgttgcc gctcaaaatc acctgcgtgc catttacctg gtactgcaaa
      241 tcatcgaaca caccgtacca aggaagcagg gccaactgat ggcgtacttg ctcagctacg
      301 ggacggttcg tttcgccaac cgttcttgca ctcgcgctag tcaccaaacc agccaacaca
      361 agagcgagaa cggggattgc cgccagagca cgtgttttca tgtgattttc ctttcgtacc
      421 tttcttactg ctgctcgctt ggacctttga gtcgactcgt gccgagtgca cttgcgcaag
      481 tagcaaccac ggggccatct gcgaactagt tgataacagg cagtctgtcg gtcctgcctg
      541 gctgagccaa gcggctgaaa tcgaccactg atggttaatc tcagccgaca gatcgaactc
      601 gaaactaaat ttgcacgcga cagccgggcg cgcgaatctt gttaacgcgc gaccgggaaa
      661 ccgaccgatt actcaacgat gcgtaattga caatcgggtc ggtggtgact atcgtgaagg
      721 cacctcgcgc cgcacagatg ccatttgcga cgcgctgagc atcaggggga tacaaatcat
      781 gcgaaaaaac gcgaattggc tgctaggcgc tgcgctggct tgcgcgagct tgtttctggc
      841 tgcggcggct tcggggcagg tgacgctcga agtgatgaat ccgcggggcg agattcctcc
      901 accgaaaacc gcggggatta gcccgcgcgg cgcaaacctc gatggcaaga aaatcgtgct
      961 gatcgacaac ggcaaattcg gagcgagcaa tttcctcgcg gtgcttgcgg acctgctgaa
     1021 gcagaagtat ccgaaggcga cggtggtgat gtatccgaaa ccggcggcgc agacgatcac
     1081 ggatctgccg aaatggtatc cgactgtgaa gcaacaaggc gatcttttcg ttttcggcgt
     1141 cggtgattga gggagctgca cgtggtgggg tgcccacgac acaatcgaaa ttgagaagac
     1201 aggaatgccc ggcgtttact tcacaaccac agaatttcta gacgatgcaa aatccgcctc
     1261 gaaggatcgc ggcatgccgg gagtccgcat gctggcgctc cccgcggaca agtactacgt
     1321 cgctcgcggg aacaagcaag aagcgcagcc aattgctgac gcgtttttcg acaaaatggt
     1381 ggacgcactc acgcgccccc tgacggcaga agaggccaag ccttcccagg cgcagaagca
     1441 gcacgaagac gcgaacatta acgtgaccgg caaagatttc ctggatgcct cgggaaaggt
     1501 gaacgaactg ttcctcgaga atcgctgggc ggacggattg ccgatagtcc cgccgacgcc
     1561 cgaattagtg aaggcgatgg aagcggggac agctctttcg ccacagaaag tgatcgggca
     1621 ggtggcgccg aagaacggca cggcaaccgt cgagaaaata gccatcaacg cggtgatggc
     1681 gggagccaaa ccggaatatt tccccgtgat tttggcggca atggcgggct tcaccgataa
     1741 gcactacgac ctgacgcacg tggtagcgtc cacgggatct ttcacgcccg tcgtcatcgt
     1801 ggacggaccc atcactcagg aacttggctt taactcgggt atcgggatgc tgggtatcgg
     1861 gtggcaggca aatagcacgg taggccgggc gctccaactg tccctgctca acattggcca
     1921 gacatggccg caggtgaatt tcatggggct caccgggcgg ctcgaggggt acaccttcta
     1981 cacgttcgcg gaggacacgg cgaagagccc gtgggagccc tacaacatga gccagggctt
     2041 tcagaaggga gacagcaccg tcacggtttc gtcggcggga aacccggcgg ttttcggcgg
     2101 cggagcggtc gcgccatgga cagcgcaagg cgttatcgat cagatggtgg cgcaaattcg
     2161 gaatcgggga gtggcttggc ctcacgccca aaagcatatc gtggtgctgc atcccgactg
     2221 cgcggtcgaa cttgcgaata aaggctacac acgcaagagt ctgcaggagt atttctacga
     2281 gcacacgcgc gtgccatacg agaagctagt cggaggactg gcggcatttg gcgaaggctc
     2341 ggaagaggca cttatccgtg cctcagtcgc cgatggccgc atccgtcccg atcgtgctca
     2401 gattttcctc gataatctga aacccggcgg ccgcgtcccc gttgtccagt cccccgagga
     2461 ttttcatatt gtggtggccg gaggatcgcc cggatacgat ctgctcttca cctatccggg
     2521 aacgaactgg gcgcaccaaa cggtgaaaat cacaggcgcc acaccaacca aaactggcaa
     2581 ctagctagtt ctttttttct gatgcttgtt gcgaagcgcc ttcgtggtgg aatccggcgc
     2641 ggagaaatta cgtgcagggt tcgaatctgg acgcggccgc acgttcgact gggcggccgt
     2701 tacgggatgg acgaaggcga gatcgaggtg gactcgatcg agccgattgg cttcccggac
     2761 atcacaccgg aattggtgcg ggcatcgggt tttctgggag tcctcgacct gctctaagtg
     2821 gccaagcacg gcggtggcga caggatctat ttgattcggt tccgctacgt gccaccgcgc
     2881 cagaaacaca aggctcggac gcggtgatcg tgatcgaggc tttgcgccgg gaaaccacgc
     2941 ggaaaccgag cgcgtctggc gccgtagatg cgctcgcatg ggggaattta catttgaaaa
     3001 aacttgcggc cctcgcgata ttggttcttt ccgccgtcat gttgctcaga gctcaggaag
     3061 gcgcgccagc tccggctgcg ccagggcaag gcgctcgtcc tggtgcacgc aactcagcgc
     3121 agaaagaggc tgaagccgcg gcggccagcc ggggaagtac catcttcaaa gagaattgcg
     3181 ccgcctgcca cggcgaaaac ctcaccggcg ggagaggccc tgacctcatt cggtcgtctc
     3241 ttgtgcggca cgataaaaat ggcgatctga tcggccccgt ggtcacgaac ggacgcacgg
     3301 agaaaggcat gccggccttt ccctttaccc aagcccaagt tgccgatctc atcgcgttca
     3361 tccaggcgca acttgcgcta ttcgatctcc atacgcgctt tagtccttat cctaacgata
     3421 ttccggcgtc gcgacttgcc acgggcacgg tcgaggcggg gaaatcctac tttgaagctc
     3481 actgctccag ttgccattcg ccaaccggcg acctcgcgca cgtcgcaagc aaatacaatc
     3541 cacccgatct cgaagttcgg ttcctctatc cttccggcgc gccttccact gcgacggtaa
     3601 cgctgcccag cggcgacaaa ttcgaaggca cgctcctgct gaacgacggc ttcaacgtcg
     3661 ccatacaggg caaagacggc tggtatcact cctggccgcg cagtgacgtg aaactcgaag
     3721 tgcatgatcc gctcgccgcg catgccgcgt tattgcaaaa ataca
//
LOCUS       DATNDT010000071         2935 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3434097, whole genome shotgun sequence.
ACCESSION   DATNDT010000071 DATNDT010000000
VERSION     DATNDT010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3434097"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1998)
                     /locus_tag="VN788_02075"
     CDS             complement(<1..1998)
                     /locus_tag="VN788_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU19351.1"
                     /translation="MKIILAAALCLGGLAAGAAPQTSTPTPAQPPAYKATGEEVLLDV
                     VVRDKKGRFVNDLKPENFEVFDNGEKRAIKSLRLVEGSEAVTSAGARSQLDPLRQIRL
                     LTLIFEGLDQDGRRLSRDAGMSLLKSELGPNVYMSIMVIDHKLEVIQPFTNDRDLLKK
                     AIARATGGANDFTADNLQVRAELEQMLGPNQSGAQSLADRAAAMSNGATAASGPGAAP
                     NGSATANAVMAQMMLKIVNSNQEDVATDAGRAIIYSLLNAVKEQYLLPGRKTILLFSP
                     GFSVPQGMEEPFRMVISVANRSNVSFYAIDSRGLTIAGLNANSVDQLANAAAGSKANS
                     TSNGSGVTMDMAQSVDRSIDSQRANTQDTLGQLARDTGGALIANTNDFKGPLRKLDED
                     IQTYYEISYNPEIRNYDGAFRRVSVKTDEADLRVQSRSGYFALPPSMTAGGQVIAAYE
                     VPLLKALDEKPVPHSFGYESTGMHFRGDTGAPTCDVVIDIPLGNLTLTEDKGSQVYQG
                     SLAYVALLKDGKGQIVKKFRSEVPVKIGADQIEAFKANSHFVSAENFDVPPGRYILET
                     AVLDGAGQKISARKSVFIVPGQSSSLGMSSVAVVRDTKAKGPATAANDPLLMADKVIT
                     PTVSPTIKKSVRQGVPFFVIVYPDKAVSAKPTLKMVFSRDGQ"
     gene            complement(2015..>2935)
                     /locus_tag="VN788_02080"
     CDS             complement(2015..>2935)
                     /locus_tag="VN788_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HXU19352.1"
                     /translation="PQPGTSMAHAMETVAECAILRSHPLLTFRANGYSYKIERKGDES
                     IYSVTNGTQTLTVPIGWAFGLGSAGQTYVFEREGVFYESFVSYYKEPNALDITMGDQK
                     LHPSNLTEAAGRLMGDRETVSCFGCHSTSSVAGAKLTLGSLIPGVQCERCHGPTEHHL
                     EGLKTGNAQLFAMKKLGSMSAEETNEFCGQCHRTWAQIAMNGPHGVLNVRFQPYRLTN
                     SRCYDVDDQRIKCTSCHDPHREVDKISTDYDRKCLACHGGGKPGAKACTVASKGCTSC
                     HMPKIDLPGAHHRFTDHNIRIVKANGAYPD"
BASE COUNT          532 a          888 c          856 g          659 t
ORIGIN      
        1 ctggccatct ctgctgaata ccatcttgag cgtcggcttc gccgaaacgg ccttatccgg
       61 gtagacgatc acgaaaaaag gaacgccctg ccgcacggat ttcttgattg tcgggctgac
      121 cgttggcgta atcactttgt ccgccatcag cagcgggtcg tttgccgccg tcgccggtcc
      181 cttcgctttg gtatcgcgga ccaccgccac gctgctcatg ccaagcgaag agctttgccc
      241 gggaacaatg aagaccgatt ttcgcgcgct gattttctgc ccggcgccat cgaggacggc
      301 cgtttcgagg atgtagcgcc ccggagggac gtcgaagttt tccgcggata cgaagtggct
      361 gttggccttg aatgcctcaa tctgatcggc gccgattttc accggaacct cggagcggaa
      421 tttctttacg atctgcccct ttccgtcctt caacagggcg acataggcga ggctgccctg
      481 gtacacctga gagcctttgt cctctgtcag tgtcaggttg ccgagcggaa tgtcgatgac
      541 gacgtcgcag gtgggagcgc ccgtgtcccc gcggaagtgc atgcccgtcg actcgtagcc
      601 gaaggagtgt ggcacgggct tttcgtcgag ggccttcagc agcgggacct cgtaagcggc
      661 gatcacctgg ccgccggccg tcatggatgg aggcagcgcg aagtagcccg agcgcgattg
      721 cacccggaga tcggcctcat ccgtcttcac gctgaccctg cgaaacgctc catcgtaatt
      781 acgaatctcc gggttgtagc tgatttcgta gtaggtctgg atgtcttcat ccagtttccg
      841 gagcgggccc ttgaaatcgt tcgtgttggc gatcaaagcg ccgcccgtgt ctctggcgag
      901 ctggccgagg gtatcctgcg tgttagctcg ctggctatcg atggagcgat ctaccgactg
      961 cgccatatcc atagttaccc cgctgccatt cgaagtcgag ttggctttgg agccggcagc
     1021 cgcgttcgcg agctggtcta ccgagtttgc gttcaggccg gcaatggtca aaccgcgcga
     1081 atcgatggca tagaagctta cgttagagcg gttcgccacg ctgatgacca tcctgaacgg
     1141 ctcttccatg ccttgcggaa cgctgaagcc gggagaaaag agaaggatgg tttttcgtcc
     1201 gggcagaaga tactgttcct ttaccgcgtt aagaagcgag tagatgatcg cccgccccgc
     1261 atcggtggct acatcctcct gattcgagtt cacaatcttc aacatcattt gcgccatcac
     1321 ggcatttgcg gtggcggagc cgttcggcgc ggctcctgga cccgatgccg cggttgcgcc
     1381 gttcgacatc gcggcggcgc ggtccgccag actttgagcg ccgctttgat tgggccccag
     1441 catctgctcc agttcagcgc gcacctgcag gttatccgct gtaaagtcgt tcgcgcctcc
     1501 ggtcgcgcgg gcgatcgcct tcttgagaag atcccggtcg ttcgtgaacg gctggatcac
     1561 ctccagcttg tgatcgatca ccatgatcga catgtaaacg ttcggcccca gttcggactt
     1621 gagcaggctc attccggcat cccgcgaaag gcggcggcca tcttgatcca atccctcgaa
     1681 gatcaacgtc agcaggcgaa tctgccgcag cggatcgagt tggctccttg cgcccgcgga
     1741 agtgacagcc tcgctccctt ccaccagccg gagggacttg atcgcgcgct tctcgccatt
     1801 gtcaaaaacc tcgaagttct ccggcttaag atcgttgacg aagcggccct tcttgtcgcg
     1861 caccacgacg tccagaagta cctcttcgcc ggtggccttg taagcgggcg gttgcgccgg
     1921 agttggagtt gacgtctgag gcgccgcgcc cgcggcaagt cctccgagac ataatgcggc
     1981 agcgagaata atcttcatgg tcctcacctt tctgttaatc cggataggct ccattggcct
     2041 ttacgatccg gatgttgtga tccgtaaacc gatggtgcgc gcccggcaaa tctattttcg
     2101 gcatgtggca cgacgtgcag cccttggacg caaccgtgca agctttcgcc cccggtttgc
     2161 cgccgccatg acatgccaga catttccgat cgtagtcggt tgaaatttta tcgacttccc
     2221 tgtgtggatc gtgacagctc gtgcatttaa tgcgctggtc gtccacgtcg tagcagcggc
     2281 tgttggtcaa gcggtagggt tggaagcgga cgttgagcac gccgtgcggc ccattcatcg
     2341 caatctgcgc ccacgtccgg tggcactgcc cgcagaactc attggtttcc tccgctgaca
     2401 tggaaccgag cttcttcatc gcaaataact gggcgttgcc cgtcttcaaa ccctccaggt
     2461 gatgttccgt tgggccgtgg cagcgttcgc actgcacccc cggtatcagc gatcccagag
     2521 tcagcttcgc gccggcaaca gagctggtcg aatggcaccc gaagcagctt accgtttcgc
     2581 ggtcgcccat cagacggccg gctgcttccg tgagattgga cggatgaagc ttctgatcgc
     2641 ccatcgtgat atcgagggca ttcggctcct tgtagtagct gacgaaactc tcgtaaaaga
     2701 cgccctctcg ttcaaacacg tacgtctgtc cggcggaacc gagtccgaat gcccatccaa
     2761 tcggcacggt cagggtctgc gttccattgg tcaccgaata aatgctttcg tcgcccttcc
     2821 gttcgatttt gtaagagtac ccattggccc ggaacgtgag cagcgggtga gaccgcagga
     2881 tcgcgcactc cgcaacagtc tccatcgcat gcgccatgga tgtgccgggc tgcgg
//
LOCUS       DATNDT010000072        12059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2691312, whole genome shotgun sequence.
ACCESSION   DATNDT010000072 DATNDT010000000
VERSION     DATNDT010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12059
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2691312"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            127..1053
                     /locus_tag="VN788_02085"
     CDS             127..1053
                     /locus_tag="VN788_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HXU19353.1"
                     /translation="MLPFKLIYADGYDLNLGAHVFPSQKFRMIRDRLLEQGIAERSDF
                     LVPEPAADEDVLRVHSREYVRKLKAATLSEAEIARLEVPYSRELVEACWLAAGGSILA
                     GQCALRDGWAANIGGGFHHACPDHGEGFCAIHDVAVAIRRLQHDGAVQRAMVVDTDVH
                     HGNGTAVIFNGDETVFTLSIHQENNYPFFKPPSTVDIDLQDGIGDDDYLGVLEKHLHK
                     AFQDFAPQILFYIAGADPYREDQLGGLALTMEGLARRDALVFEYARRNGVPVASALAG
                     GYARRVADTVSIHVATITAARDAARQSTRAGA"
     gene            complement(1316..2710)
                     /locus_tag="VN788_02090"
     CDS             complement(1316..2710)
                     /locus_tag="VN788_02090"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXU19354.1"
                     /translation="MLTSLATWPNPSPSNNVLARRRAYKIPSMRVNFFRRHGASWALA
                     LLIGLLLSFSARAQSSAEAGSGKLASVRVTGSSRFRSDQIAPTTGLKSGATVTKDDLQ
                     RGADRLAQLGPFTGVHYRYGDTDAGVVVEYQVTDAPAVPVWFDNFPWFSDAELISAVK
                     SSVPLFDGTAPDHGTLLDDISDALQLALASRNVHTSVTHSFVTAPGGSEKVQQFRVDG
                     MDLNVASVEFSDTLARSDRAIQARLSDLVDHAYSRTVIELFEFEQVRPVYLAQGFLRV
                     RFKPPSTRLVGSGPGARVAVTITVEPGSAFTWNGVTWSGNTVILSPELNALVTLKPGD
                     PANEMKIQPIWESVHDDYARRGYLDATVTPHAQFDDTAKHVSYTATISEGPQFRMGKL
                     VLTGLSIEGERRIRAAWQIEPGAIFDKDVYDEFITSGIKQAFTGLPFHYEKLGRFLQE
                     DPKAGTVDVLLDFQ"
     gene            complement(2664..4520)
                     /gene="glmS"
                     /locus_tag="VN788_02095"
     CDS             complement(2664..4520)
                     /gene="glmS"
                     /locus_tag="VN788_02095"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HXU19355.1"
                     /translation="MCGIVGYIGPKRVVPLILDGLKRLEYRGYDSAGIAVVGDNGKLE
                     IRRAPGKLRNLEESIRLTPLDGLYGIGHTRWATHGRPTEENAHPHRDCTGQIVVVHNG
                     IIENYVELKNQLLAEGHKFITETDTEIIAHLVEKFSKDASLEDAVLKTVKIMTGAYAM
                     VVVSAKDPNKIVAARLGPPIVVGLGEHEFFVASDIPAILQHTRSIFFLGDGEIAVLTP
                     QGVRLFDFESHPVSRAPQHITWDPIMAEKGGFKHFMQKEIFEQPRAVRDTLLGRISQE
                     TGQVFLDKMDISEEEFRNFRDVKIVACGTSWHAGLAGKFMIERLAHLPVEVDYGSEFR
                     YRDPILDKHSLIIAISQSGETADTLAAEREARQKGAKVLSICNVVGSMLTRESSGTIY
                     THAGPEIGVASTKAFTGQLTALLLTALHLGQVRGKLASAASKSLMQEVTRIPHKIETI
                     LQHDAEMEDLARQFFRYTDFLYLGRGIHYPIALEGALKLKEISYIHAEGYPAGEMKHG
                     PNALIDENLPVVMIATRDESDPASMTRYEKTISNLQEVKARDGIVIAIVTHGDKVACE
                     TADHVIEIPPAPDLIAPILEIVPLQLLAYHIAVRRGCDVDQPRNLAKSVTVE"
     gene            complement(4671..4913)
                     /gene="purS"
                     /locus_tag="VN788_02100"
     CDS             complement(4671..4913)
                     /gene="purS"
                     /locus_tag="VN788_02100"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HXU19356.1"
                     /translation="MKAHVWVMLKSTVLDPQGQTIHRALSSLGYAKIQNVRQGKFFVL
                     QLDGLSRDEAKSQVERIAKDVLTNPVIEEFRFEILE"
     gene            complement(4974..6065)
                     /gene="add"
                     /locus_tag="VN788_02105"
     CDS             complement(4974..6065)
                     /gene="add"
                     /locus_tag="VN788_02105"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HXU19357.1"
                     /translation="MDQCWTPTASRFLNQIVSEAGQQRTVLDTLPKAELHLHLEGSIR
                     PETAVELAGRHGTRLTLGDVTARYNYSNFAGFIESFKWVTSFLREPEDYAFITRKLLE
                     ELISQRVVYAEITISAGVMLRRMQNVEANFNAIREAADGILYRRLRTAWIFDAARQFG
                     PEAAMEVSRFASKLHRSGLVAFGMGGDELSFPAAHFRPAFDLARSEGLRVVCHAGEIG
                     GPESVRDAAEILGAERIGHGIAVVHDEALAKSLANGHVVLEICPSSNLATGALAKQTG
                     KPEASLAEHPLRKLVELGSLVTISTDDPAMFHTDLLAEYSHAESLGLSKEQLLQIAEQ
                     SFNAAFLPPIDKRKLLEDFRAAAKAGALL"
     gene            complement(6026..6889)
                     /locus_tag="VN788_02110"
     CDS             complement(6026..6889)
                     /locus_tag="VN788_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19358.1"
                     /translation="MRPLTGPSMRYGFLSALFLALLVFLMAASSSFGWGESAERTMVE
                     KAVDTLPAEMLPFFQAHRPFLVEHVADAAASEAKNPSERRNDFIQLDHYGQFPFASLP
                     RSYTEAVAKFGKRSVEAHGQLPWQIGLYSQKLTEAFRTHDWNNAELSGAILAHYVAAA
                     HNPFDTTINSDGKLSAQPGVNERFSHSLVDRYQRFFYVKPNEAAFVADPTDHAFEMCL
                     SAHSWLESILLADRRAYAGLSGYNEEYYDRFYAQEGAVLIRQLTDASTDIGSYWMTAW
                     INAGRPQLPGS"
     gene            complement(6903..8444)
                     /locus_tag="VN788_02115"
     CDS             complement(6903..8444)
                     /locus_tag="VN788_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXU19359.1"
                     /translation="MDPQLFRTKSIDQLISDADRPEKRLRKTLGWLSLTSLGIGAVIG
                     SGIFTIIGTAIAGQKFKASSVLNAPLLEYLIYHSASFGRPGAGPAIALSFALVGIVCC
                     FAALCYAELAAMIPIAGSAYTYTYATMGELIAWIIGWDLILEYAVSNMAVSVGFSAHM
                     VALFDWFNFHPNLRWITPAYLPGGLSDLQGKTLYDPGWHFGFNWPAFVIVMLLTIVLV
                     RGIRESARTNNVMVLLKIAAILIFIGVASPHIRTPNWHPFAPNGWPGILTGGSIIFFT
                     YIGFDSVSTAAEECKNPQRDLPIGIIATLVICTVLYIGVVVVLTGLVPWQTLVDDAAP
                     VVNTMNRLHFGNVRLAVLIGALLGMISSLLVFQLGQARVWFAMSRDGLLPGIFSRVHP
                     RYRTPDFSTWVAGFLVGIPAGLLDIGTLADLSNIGTLFAFALVGGGVLILRYREPDRP
                     RPFRAPGGLLAPIMTIVTCLLLMAGLPITNWVRFFVWLLIGLILYHFYGRKHSGLSTA
                     KIEAA"
     gene            complement(8584..10875)
                     /locus_tag="VN788_02120"
     CDS             complement(8584..10875)
                     /locus_tag="VN788_02120"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HXU19360.1"
                     /translation="MSNFPNASPLLFGLNERQRDAVLATEGPVLVLAGAGTGKTRVIT
                     YRVAHLIEQGVPPESILAVTFTNKAAGVMRDRISELLRSSGLGAVDVWVSTFHSFCAR
                     LLRREARHLGLPRDFAIYDDEDQTSAVKRALMQLGFTAEDYPPRSLRAQISHAKNHGI
                     TPDEMESQAAQARDKDRQDAANIFRVYNDILRKAAAIDFDDLLLRAVDLLRDHADVRA
                     TWNRRFQYLMVDEFQDTNQAQEELVRLLAGTRKNVCVVGDEDQSIYGWRGARAGNLKR
                     FTQDFPGAKVIRLEENYRSTQTILDAAAAVVKNNSDRLGKNLQATLGSGDRLRFFEAP
                     DSIAEAEFICEDLSALVRGDTDARVAVLYRTGAQSRSFEEVLRRLGIRHRVVGGFSFY
                     ERAEVRNALAYIRLLFHPEDDVALLRILNVPPRGIGAVTIAALETQARETGKSLWKVI
                     DDRDSLVGKKSAGALNYFRQMIEDLQAERDDLAPAQLTERVLEQSGYLDWVEQQDNLE
                     HTSRAENLRELSNAMSEAAAQGQTLEDLLDRAALTSDSDEYDVTIPVSLMTLHSAKGL
                     EFDAVYLAGLEEGVLPHSRSFNTNADIEEERRLFYVGMTRAKKSLVLSRATYRRTYGE
                     DRLRASLPSRFLAEIPADLIEAAEGSQSEPGEARRYEPDPEYSAGYSYRGRSAHYSRS
                     GYVGSKSIASAYGRAPKPSRSSAPDRASGDSLVGTRVRHSKFGIGTIISVEGEGDDRR
                     LTVSFQDYGPKKLLERYANLQIA"
     gene            complement(11042..>12059)
                     /locus_tag="VN788_02125"
     CDS             complement(11042..>12059)
                     /locus_tag="VN788_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU19361.1"
                     /translation="ATKKIEMIDTCYGTHHLLFASDANDTLWTSSGGGGGVVGWLNTK
                     MWDQTHDAAKSIGWTALVLDTNGNGKRDAYVEGEQRVATTPGGESLGASSAVNVSTDP
                     TKDTRLNAAFYGIGIGTDGMVWGTVLGFPGGIVRLNPGTNPPATALAEYYEVPWKDPK
                     APVWGFSPRGMDVDSNNVVWVALASGHLASFDRRKCKGPLNGPKATGRQCPEGWTLYQ
                     TPGPKFKKVTGSGSDDSHYYDWVDRFDTLGLGKDTPIVTGNDSDSLIALVKGKFVVLR
                     VPYPLGFFAKGMDGRFDDPNAGWKGKGVWTTWGTRAPFHSETGKGTFPKVVHFQIRPN
                     PLAH"
BASE COUNT         2577 a         3358 c         3640 g         2484 t
ORIGIN      
        1 ggtaactttg gcgcgttgca tctctgcgag cctaccacag cgatgttgca gacgcgaatg
       61 cggtatgact acaccagttt tgtggggttt gcgtcggcag agggttcgag gtaggctggc
      121 gaaattatgc tgccgttcaa attgatttac gccgacggat acgatttgaa tcttggcgcg
      181 cacgtcttcc catcgcaaaa gtttcggatg atacgcgacc gtctgctgga gcaaggaatc
      241 gccgagcgca gcgattttct tgttcccgag cctgcggcgg atgaggatgt gctgcgcgtc
      301 cattcgaggg agtatgtgcg gaagctcaag gcagcaacgc tgagcgaggc cgaaattgcg
      361 cggctggagg tcccgtattc aagggaatta gtggaagcgt gctggctggc ggcgggagga
      421 tcgattctcg cgggccaatg cgcgctgcgg gatggctggg ccgcgaatat cggcggaggg
      481 tttcaccatg cgtgtcccga tcatggcgag ggattttgcg cgattcacga tgtggccgtg
      541 gcgatccggc ggcttcagca cgatggagcg gtccagagag caatggtggt cgatacagat
      601 gtgcaccacg ggaatgggac ggcggtgatt tttaacggag acgagacagt attcacgctt
      661 tcgatccacc aggagaataa ttatccgttc ttcaagccac cgtccacagt ggacatcgat
      721 ttgcaggacg gcatcgggga cgacgattat cttggggttc tcgaaaagca cctgcataaa
      781 gcttttcagg attttgcgcc gcagattttg ttctatatcg cgggcgccga tccttatcgc
      841 gaagatcaat tgggcgggct ggcgcttacg atggagggac tggcgcgaag agacgcattg
      901 gttttcgaat atgcgcggcg aaacggcgta cccgtggcga gcgcgctggc gggcggttac
      961 gcgcggcgcg tggcggacac cgtgagcatc catgtcgcaa cgattacggc agcgcgagat
     1021 gcggcgcggc aatcgacgcg cgcgggagcg taacgccgac gaatgcgagg agcacgccgg
     1081 gcaagccccg cggcaaacag cggcggcgag gcgccgcact ccacatggcc ccgggagcgt
     1141 aacgcccccg gcgaacggga tctggctgcc ttgatgatgg gctttgcctg tgcagcgcgg
     1201 cgagtggcca acaatcacgc caagtagctt tgccgggcac agcatgctgt gcccctacga
     1261 atgccacggc gaatgcgacg cgacgctcgg cgcgtcgaaa acagcgatcg gaaaattatt
     1321 ggaagtcgag caggacgtct accgtgccgg ctttgggatc ctcctgcaag aagcggccca
     1381 gcttctcgta gtggaagggt aggccggtga aggcctgctt gatgccggaa gtaatgaatt
     1441 cgtcgtagac atccttatca aaaatggcgc ccggctcgat ttgccatgcg gcgcgtatgc
     1501 ggcgctcgcc ttcgattgaa agcccggtga ggaccagctt gcccatgcga aactgaggac
     1561 cctccgagat cgtagcggtg tagctgacgt gctttgccgt atcgtcgaat tgagcatggg
     1621 gggtgacggt ggcgtccaaa tagccgcgac gtgcatagtc gtcgtgcacc gattcccaga
     1681 tcggctggat tttcatttcg ttggctgggt cgccgggttt cagggtgacg agcgcattta
     1741 gttccggcga taaaatcacc gtgtttccgc tccaagtaac tccgttccac gtgaaggccg
     1801 atcccggctc gacggtaatc gtgaccgcga cgcgagcgcc agggccgctg cctacaaggc
     1861 gcgtgctggg cggcttgaac cgcacgcgga ggaagccctg cgcgagatag acggggcgaa
     1921 cctgctcgaa ttcgaaaagt tcgatcacgg tacgggaata ggcgtggtcg acgagatcgg
     1981 aaagccgcgc ctggattgcc cgatcgctcc gcgcgagcgt gtcgctaaat tcgacactgg
     2041 cgacattcaa gtccatacca tcaacgcgga attgctgaac tttttcggag ccaccgggcg
     2101 cggtgacgaa cgagtgcgtg acgctggtat ggacattgcg ggaagcaagc gcgagctgga
     2161 gcgcgtcgga aatgtcgtca agcaaagtgc cgtggtcggg ggcggtgccg tcgaataaag
     2221 gcacggaaga ttttacggcg gagatcaatt ccgcgtcgct gaaccaggga aaattatcga
     2281 accaaaccgg aactgccgga gcgtcggtga cctgatattc gacgaccacg ccggcgtccg
     2341 tatcgccgta gcggtaatga acgccggtaa aggggccgag ttgggcgagg cgatcggcgc
     2401 cgcgctgcaa atcgtctttc gtgacggtgg cgccggactt gagacctgtg gtcggcgcga
     2461 tctgatcgga tcggaacctg gatgatcctg tgactctgac cgaggcgagc ttcccggagc
     2521 cggcttccgc ggacgattgc gcacgcgcgg aaaatgagag aagcaggccg atgagcaggg
     2581 cgagggccca tgaagcgcca tgccgccgaa agaaattgac gcgcatagaa ggtattttat
     2641 acgcgcggcg gcgagcgagc acgttattcg acggtgacgg atttggccag gttgcgaggc
     2701 tggtcaacat cgcaaccgcg gcggaccgca atgtggtacg caagcaactg tagcgggacg
     2761 atttcgagaa tgggcgctat caaatcaggt gcggggggaa tttcgatgac gtggtcggcc
     2821 gtctcgcacg ccactttatc gccatgggtt acgatagcaa ttacgatgcc gtcgcgagct
     2881 tttacctcct ggagattgga gatggttttt tcgtagcgcg tcatcgaagc cggatccgat
     2941 tcatcgcggg tggcgatcat gactacggga agattttcgt cgatcagagc gttcggaccg
     3001 tgcttcatct cgccagccgg gtaaccttcg gcgtgaatgt aggaaatttc cttgagcttc
     3061 agtgcccctt cgagagcgat cggatagtgg attccgcgac caaggtacag gaaatcagta
     3121 tatcggaaga actgacgggc gagatcttcc atctcggcat cgtgctggag aatggtttcg
     3181 atcttgtgcg gaattcgagt gacctcttgc atcaggctct tggatgcggc actcgcgagc
     3241 ttgccgcgca cctggccgag gtgaagtgcg gtgagcagga gcgcggtgag ctggccggtg
     3301 aaggccttcg ttgaggcgac gccgatctca gggccggcgt gcgtgtagat cgtgccgctg
     3361 gactcgcgcg tgagcatgga gccgacgacg ttgcaaatcg aaaggacttt cgcgcctttc
     3421 tggcgggctt cgcgctccgc agcgagagtg tccgccgttt cgccggactg gctgatggca
     3481 atgatcaggc tatgcttatc gagaatcgga tcgcggtaac gaaattcgct gccgtaatcg
     3541 acctcgacgg ggagatgagc gaggcgctcg atcatgaatt tgccggcgag tcctgcgtgc
     3601 caactggtgc cgcaggcgac aatttttacg tcgcggaaat tgcggaactc ttcctcggag
     3661 atgtccatct tgtcgagaaa tacctggccg gtttcctgcg aaattcggcc gagcagagtg
     3721 tcgcggacgg cgcggggctg ctcgaaaatt tccttctgca tgaaatgttt gaagccgccc
     3781 ttttcggcca tgatcgggtc ccaggtgatg tgctggggcg cacggctgac cggatgactt
     3841 tcaaaatcga agaggcgaac gccttgcgga gtgagcacgg ctatttcgcc atctcccagg
     3901 aaaaaaatac tgcgggtatg ttggaggatg gcgggaatat cactagccac gaagaattcg
     3961 tgctcgccga gaccgacaac aatcggtgga cctaaccgcg cggcgacaat tttgttcgga
     4021 tctttcgcag agacgacgac catcgcgtag gcgcccgtca ttatctttac ggttttgaga
     4081 acggcgtctt ccaaggacgc gtcctttgaa aatttctcga cgagatgcgc gatgatttcg
     4141 gtatcagttt ccgtgatgaa cttgtggcct tccgcgagga gctgattttt cagctcgacg
     4201 tagttctcaa taattccatt gtgcacgacc acaatttggc cggtgcaatc gcggtgaggg
     4261 tgggcgtttt cttcggtcgg gcgcccatgc gtggcccaac gcgtgtgccc gatgccatac
     4321 aggccgtcga gcggagtgag tcggatggat tcctccagat tacgcaattt tcctggcgcg
     4381 cgccgaatct cgagtttccc gttgtcgccc acaacggcga ttcccgccga atcgtagccg
     4441 cgatattcga gacgcttcag gccatcgagg atcaacggca caacccgctt cggtcctatg
     4501 tacccgacga ttccacacat tagacaatgc tcctagaggt agctattcca gggcccgtcg
     4561 cgacagaggg gctccaacta gggtagcaca atgaaggcgg gcgtccggga ctgtccctgt
     4621 ggaactgttc cgaagcgggt aatcgcaagc gaaggacgac acactgaaaa ttattcgagg
     4681 atttcgaagc gaaattcttc gatcacgggg ttggtgagca cgtccttcgc gatccgctcg
     4741 acctgactct tcgcttcgtc gcggctcaac ccgtcgagct gcaggacgaa gaattttccc
     4801 tggcggacgt tttggatctt ggcatagcca agagaggaaa gcgcacggtg gattgtctga
     4861 ccttgcggat cgaggactgt ggatttcagc atgacccaga cgtgagcttt catggatctc
     4921 gttttctcaa ctgcaagatg gacgtacgcg cactgcgttt cggcgccgca agattatagc
     4981 agggcccccg ccttggccgc ggcgcgaaaa tcctccagga gcttgcgttt gtcgatcgga
     5041 ggcaagaacg cggcgttaaa gctctgctcg gcaatttgca acagctgctc tttcgatagg
     5101 cctagcgatt cggcgtgaga atattctgcg agcagatcgg tgtgaaacat cgccggatcg
     5161 tctgtggata tggttacgag cgaacccagc tcgacaagct tgcgaagcgg gtgctcggca
     5221 agcgatgctt ccggcttgcc ggtctgcttg gcaagggctc cggtggccag attactcgac
     5281 gggcaaatct caagcaccac gtgaccgttg gcgagcgatt tggcgagggc ctcatcgtgg
     5341 actacggcga tgccgtgacc tatgcgttcg gcgcccaaga tttcagcggc gtcgcgcacg
     5401 gattccggtc ccccgatttc accggcgtga caaacgaccc gcaggccttc gctgcgggcc
     5461 aaatcgaatg caggccgaaa atgggccgct ggaaatgaca gttcgtcacc gcccatccca
     5521 aacgcaacga ggcccgatcg gtgtaacttg gaggcgaatc gcgatacttc catagcagcc
     5581 tctggaccaa actgccgggc ggcgtcgaat atccatgcgg tacgaagccg gcggtaaagg
     5641 atgccgtcgg cggcctcgcg tatggcgttg aaattcgctt ccacgttttg catccgccgc
     5701 agcattacgc ctgccgaaat cgtgatttcg gcgtagacga cgcgctggct gataagttct
     5761 tcaagaagtt ttcgggtgat gaaggcgtaa tcttccggtt cacgcaaaaa agaggtgacc
     5821 cacttgaaac tttcgataaa gcccgcgaag ttagaatagt tgtaacgcgc ggtaacgtcg
     5881 cccagcgtca atcgagtgcc gtgacggcca gcgagctcaa cggccgtttc cggccggatg
     5941 gacccttcga gatgcaagtg caattcagcc ttggggaggg tatccaatac ggtccgttgc
     6001 tgcccggcct cgctcacaat ttgattcaag aaccgggaag ctgtgggcgt ccagcattga
     6061 tccacgcggt catccaatag gaaccgatgt cggtggaagc gtccgtgagc tgccggatca
     6121 aaaccgcgcc ttcctgagcg tagaagcgat cgtaatattc ctcattgtag ccggacaagc
     6181 ccgcataagc gcgacggtcc gctagaagaa tactttcgag ccaggaatgc gcgctcaggc
     6241 acatctcgaa cgcatgatcg gtaggatcgg caacaaaggc agcttcattg ggcttcacgt
     6301 aaaagaaacg ctgataacgg tctacaaggc tatggctgaa tcgttcgttg actccgggct
     6361 gagcggacag tttcccgtcg ctgttaatcg tggtatcgaa gggattgtgc gctgcagcta
     6421 cgtagtgcgc aagaattgct ccggaaagtt cggcattatt ccaatcgtgg gtgcgaaatg
     6481 cctcggttaa cttttgacta taaaggccga tttgccatgg aagttgccca tgcgcttcga
     6541 cgctccgctt gccaaacttt gcaactgctt cggtgtaaga gcgcggcaga cttgcgaacg
     6601 gaaactgccc atagtggtcg agctggatga aatcgttgcg acgttccgat gggttcttcg
     6661 cttccgaagc ggccgcgtca gcaacgtgct ctaccaggaa tggccggtga gcctggaaga
     6721 acggaagcat ctccgcgggc agtgtatcga cggccttctc caccatagtg cgctccgcac
     6781 tttcgcccca gccaaaggac gatgacgccg ccatcaggaa cactaggaga gccaggaaaa
     6841 gcgcactcag aaatccgtac cgcatcgatg ggcccgtcaa tggccgcatc tgtggaatct
     6901 ccttacgctg cttcaatttt tgccgtggaa agcccgctat gtttgcgccc gtagaaatga
     6961 taaaggatca gtcctatcag gagccaaacg aaaaagcgga cccagttcgt aatcggaagc
     7021 ccagccatca aaagcaagca tgtgacaatt gtcatgatgg gtgcaagcag tcccccgggc
     7081 gcccgaaatg ggcgcggccg gtcaggttcg cggtaccgca gaatcaatac gccgccgccg
     7141 accaacgcga atgcgaaaag cgttccgatg ttggagaggt cggcaagcgt accgatgtcc
     7201 aacagaccgg ccgggatgcc caccagaaac ccggctaccc acgtggaaaa atccggtgtg
     7261 cggtagcgcg gatgtacgcg gctaaaaatg ccgggtaaga ggccgtcgcg cgacattgcg
     7321 aaccacacgc gcgcttggcc aagttgaaaa acgaggaggg atgaaatcat gcccagcagc
     7381 gcaccgatca gcactgccaa ccgcacgttg ccaaaatgga ggcgattcat cgtgttgacg
     7441 actggcgccg catcatcgac cagcgtctgc cacggcacca gacccgtcag caccacgacc
     7501 acaccgatgt aaagcaccgt gcagatcacc agcgtcgcga tgattccgat cgggagatcg
     7561 cgctgagggt ttttgcactc ttctgcggcg gttgagactg agtcgaaacc gatgtaagta
     7621 aagaagataa tcgagccgcc agtgaggatt cccggccaac cgttaggcgc gaacggatgc
     7681 cagtttggag tgcgaatgtg gggtgaagcg accccgatga agatcaatat tgcggcaatt
     7741 tttaacaaca ccataacgtt gttggtgcga gccgactccc tgattccgcg cacgagcaca
     7801 atcgtgagca gcatcacaat gacgaatgca ggccagttga atccgaagtg ccagccggga
     7861 tcgtagagcg tcttgccttg cagatcggag agtcctccag gcaaatatgc cggagtgatc
     7921 cagcgcagat tgggatgaaa attgaaccaa tcgaatagcg ccaccatgtg cgccgaaaac
     7981 ccgacgctga cggccatatt cgacactgcg tattcgagaa ttaaatccca gccaataatc
     8041 caagcgatga gttcgcccat cgtcgcgtac gtataggtat aggcgctgcc tgcgatcgga
     8101 atcatggccg cgagttcggc atagcaaagg gccgcaaagc agcagacaat tcccacaagc
     8161 gcgaacgaca gcgcaatcgc cggacccgcg ccgggcctgc caaatgacgc ggagtggtag
     8221 atcaggtatt ccagcagtgg agcattcagc acggagctgg ccttgaactt ctggcccgca
     8281 atggccgtgc cgatgatggt aaagattcca gaaccgatta ccgctccgat tccgagagag
     8341 gtaagactca gccagccgag agttttcctg aggcgttttt cgggccggtc ggcgtcagag
     8401 atgagctggt cgatgctctt ggtgcggaaa agctgtgggt ccaatcgcca ccttcccccg
     8461 tgcgaccgag gagaactcta ccttgagaga cccctcggcg caacctgtgc ttcaggtacg
     8521 cggggaatgc actcactgct tatttgggag ccatgggact gaaccgtttt ctcgtcttgg
     8581 cagtcaagcg atctgcaaat tggcgtaacg ttcgagaagc tttttgggac cgtaatcctg
     8641 gaagcttacg gtaagtcttc tatcgtcgcc ttcgccctcc acgctgatga tcgtgccgat
     8701 cccgaatttc gagtggcgca cgcgagtgcc cactagggaa tcgcccgacg cgcgatcagg
     8761 cgcggaggaa cggctgggtt ttggcgcgcg accgtaggcc gaagcgatgg atttggaacc
     8821 cacgtaaccc gatcggctgt aatgagcgct acggccccgg tacgaatatc ccgccgagta
     8881 ttcgggatca ggttcatagc ggcgcgcttc tccgggttcc gattgcgaac cctcggcggc
     8941 ttcgatcaag tcagcgggaa tttcggcgag aaagcgcgag ggaagcgaag cgcgcaaacg
     9001 gtcttcgcca taggtcctgc gataggtcgc tcgtgaaagc acgagcgatt ttttggcgcg
     9061 cgtcatccct acatagaaca atcgccgctc ttcctcgatg tccgcatttg tattgaatga
     9121 acggctatgc ggcaacacgc cctcttcgag tcccgcgaga tatacggcgt caaattccag
     9181 gcctttggcg ctgtgcagag tcataagcga gacgggtatg gtgacgtcgt attcgtccga
     9241 atcgctggtg agcgcagcgc gatcgaggag atcttcgaga gtctgtccct gcgcagcggc
     9301 ctcggacatg gcgtttgaga gttcccgcag attttcggcg cgcgaagtgt gttcaaggtt
     9361 gtcctgctgc tcgacccaat cgaggtaacc tgattgttcg aggacgcgct cggtcagttg
     9421 tgcaggggcg agatcatcgc gctcggcctg gaggtcttcg atcatttggc gaaaataatt
     9481 cagcgcgccc gcggacttct tgccgaccag agaatcgcga tcatcgatca ctttccacag
     9541 cgatttgccg gtttcgcgcg cctgtgtttc cagagcggcg atggtgacag cgccaatccc
     9601 gcgaggtggg acattcagta tgcgtaacaa tgcaacatca tcttcaggat ggaaaagcag
     9661 tcgaatataa gccagagcgt tgcggacttc cgcgcgttcg tagaagctga atcctccgac
     9721 gacgcggtgt cggatcccca gccggcgcaa aacctcctca aacgatcgcg attgcgcgcc
     9781 cgtgcgatag agaacagcaa cacgcgcgtc cgtatcgccg cggaccagcg ccgagaggtc
     9841 ttcacaaatg aattccgctt cggcgattga gtccggcgcc tcgaaaaagc gcagcctgtc
     9901 gcccgacccc agtgtggcct gcaaattctt gccaagccga tctgaattgt ttttgaccac
     9961 ggcggccgcc gcatcgagga ttgtctgcgt ggagcggtaa ttttcctcca ggcgaatcac
    10021 cttggctccc gggaaatcct gcgtgaagcg cttcaagttc ccggctcggg ccccacgcca
    10081 gccataaatc gactggtctt catcgcccac gacgcacacg tttttccgtg tgcctgccaa
    10141 gagacgcacg agttcttcct gcgcctggtt cgtgtcttga aattcgtcca ccattagata
    10201 ttggaagcgc ctattccaag tcgctcggac atcggcgtgg tctcgaagca ggtccaccgc
    10261 acgcagaagc aggtcgtcga aatcaatcgc ggcggccttg cggagaatat cgttgtagac
    10321 gcggaagatg ttggcggcat cttggcgatc tttatcgcgc gcctgggcgg cctgggactc
    10381 catttcgtcc ggcgttatgc cgtgattctt agcatggctt atttgggcgc gcaagctgcg
    10441 cggaggatag tcctctgccg tgaaaccaag ttgcatcaac gcccgcttca cggccgaggt
    10501 ctgatcttcg tcatcgtaaa tagcaaaatc gcgcgggagg cccaaatgcc gcgcttcgcg
    10561 acgcaacagc cgcgcacaga aagagtgaaa ggtcgaaacc cagacgtcta cggcgccgag
    10621 tccgctggat cgaaggagtt cgctgatcct gtccctcatc acgcccgcgg ctttgttcgt
    10681 gaacgtgacg gcgaggatcg attcaggggg aacgccctgc tcaatcaggt gggccacgcg
    10741 gtaggtgatc acgcgagtct taccagtgcc ggcacccgca agaacgagga caggcccctc
    10801 cgtcgcaagc acggcatcgc gctggcgctc gtttaacccg aagaggagtg gcgatgcgtt
    10861 tggaaaattg ctcaagttcg aattctagcc gacgaaatac aattgagcaa tttaggcgga
    10921 caaggtagcg acggacacac acaatgcgcc gcccaatctg atcaacgcgg aaaccctcag
    10981 atttaagatc gccttgagcg caggggacaa tgcacatttc tcctgctatc tccgcggcca
    11041 gctagtgtgc gaggggattt gggcgaatct ggaagtggac gaccttcgga aatgtgcctt
    11101 ttccggtctc gctgtgaaat ggtgcgcgcg ttccccatgt ggtccagact cctttgcctt
    11161 tccagccggc attcgggtca tcgaagcgcc cgtccattcc tttcgcgaag aagccgagcg
    11221 ggtagggaac gcgcaggacc acgaattttc ccttcaccaa agcaatcagg gaatccgagt
    11281 cattacccgt aacaatcggc gtgtccttgc ccagtccgag cgtgtcgaac cgatctaccc
    11341 aatcgtaata gtgagaatcg tcgcttcccg accccgtcac ctttttgaac ttcggtccgg
    11401 gcgtctggta caacgtccat ccttcaggac actgccgccc tgtcgccttc ggtccgttga
    11461 gcggcccttt gcacttgcgc cggtcgaagc ttgcgagatg cccactcgcc agcgccaccc
    11521 aaacgacgtt attgctgtcg acgtccatcc cgcgcggcga aaagccccat acaggagcct
    11581 tcggatcctt ccacggtact tcataatact ccgccagagc ggtcgccgga ggattggttc
    11641 ccgggttcaa ccgcacgatt cctccgggaa atcccagcac cgttccccag accatcccat
    11701 ccgtgccgat cccgataccg tagaacgctg cattgagccg tgtgtccttc gttggatcag
    11761 ttgacacgtt cactgccgac gaagcgccca ggctttcacc gcccggcgtc gtagccaccc
    11821 gctgctcgcc ctccacatat gcatcgcgct ttccattacc attggtgtcg agcaccagag
    11881 ccgtccaccc gattgatttg gcggcatcat gagtctggtc ccacatcttg gtattcagcc
    11941 atccgaccac accgccacca cccccactgc tggtccatag cgtgtcattg gcgtcgctgg
    12001 cgaaaagcaa atggtgcgtc ccgtaacatg tgtcgatcat ctcgatcttc ttcgttgcg
//
LOCUS       DATNDT010000073         3240 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3784166, whole genome shotgun sequence.
ACCESSION   DATNDT010000073 DATNDT010000000
VERSION     DATNDT010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3784166"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VN788_02130"
     CDS             complement(<1..254)
                     /locus_tag="VN788_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19362.1"
                     /translation="MAIKRKIIGGFLACACAALPCAHAQEQALTRRTDPALSHPADTP
                     PAMQMVDLTVPQGTPLQVALAKETRVKKAGQLIQGRIVEPV"
     gene            430..1077
                     /locus_tag="VN788_02135"
     CDS             430..1077
                     /locus_tag="VN788_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03914.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HXU19363.1"
                     /translation="MKRPREDRPSAKVQRRPPVPPNPTLPRLQDAARNCRACDLWRNA
                     TQTVFGAGPPRSKIMLVGEQPGNDEDLDGRPFVGPAGKLLDRALIEAGIDRTEVYVTN
                     AVKHFKWEPRGKRRIHKKPNSAEIAACRPWLDAEIAVVRPQVIICMGATAAQSLFGRE
                     FRVTKHRGEFLESSLAPTVTATIHPSAILRAPDEETRRMEFAKFVADLKKASQRH"
     gene            complement(1105..2196)
                     /locus_tag="VN788_02140"
     CDS             complement(1105..2196)
                     /locus_tag="VN788_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXU19364.1"
                     /translation="MIRVIRRIFVVLAAVVVLAAAGGLCYRAWRQHHIANEMKINSRN
                     GINEAKFVEINGAQEWITIRGQDRDNPVILFLHGGPAEANSPFAELYLPFEENYVFVQ
                     WDQPGAGMTFIKAGDHQPKLTLDGAVSDGIAVAEYLRTELHAPKIILIGQDWGAVVGI
                     RMIEKRPDLFKLLVGTGLVVGDQARQVVLYRYALSHATGNRDEKMLAALKQVGPPPYS
                     TLERYGRFQDCCRNPFWPPDDIAGINRLRGLLVVTPSLSISQAYGWYKALRTDEVKLD
                     AIAVNMADLRDTDTKFSVPAFFIQGENDNVAPTSLVADYAAKIQAPAKKLDIVPDAGH
                     FVMWTHPREFLSALREDVPMASADNHLGN"
     gene            complement(2193..>3240)
                     /locus_tag="VN788_02145"
     CDS             complement(2193..>3240)
                     /locus_tag="VN788_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXU19365.1"
                     /translation="KGEDREMGSQPQTHVNPERLMQITWSFAQPLAIGSAIQNRVFDV
                     LDSGPKSVAEISKETGASVRGLTAVMNLLAHINLLAKLSDGRYSLTPESAAFLVSSKP
                     GYMGGFIRQVGTRLAEGFLQLPEVMRTGTPPEGEESERPEAAAFFEELVVDIMPLAFP
                     PAQKLAEVLGLSRATQPISVLDLASGSGAWGIALAQASPQVSVTAVDWPSVLTVTRRI
                     AGRYGVAERFHDLGGDLFKVDYGKDHSIATLGNILHMLGPTENKTLLKKTFDALAPGG
                     TIAIGEFLVDDDRNGPLVPLTFAINMLLHTKNGNAYTFAEIRSWLEEAGFVNARLQEA
                     PGPSPLVLAEKPRP"
BASE COUNT          647 a         1014 c          899 g          680 t
ORIGIN      
        1 acgggttcaa caattcgccc ctgaattagc tggcccgcct tcttgacgcg cgtttctttg
       61 gcgagtgcga cttgcaaggg cgtaccctgc ggcaccgtca gatccaccat ctgcattgcc
      121 ggaggtgtat cggctgggtg cgaaagcgcg ggatcagtgc gccgagtgag agcttgttcc
      181 tgcgcatgcg cgcatggcaa ggccgcacat gcacatgcaa gaaaaccgcc gattattttg
      241 cgcttgatag ccatgtcgac ttccctgcgg cgtgacttcc gttcggagat tcgttcagaa
      301 cttgaacgtc gctgatacgc cgcaattccg gtcggatttg cgcagtcgct accctcggcg
      361 ggcgcctcta ttgtacccct tcgagcttgg caactgcagc gatcgcttac ccccgtgcat
      421 caaaacgtcg tgaagcgacc acgcgaagat cgtcctagcg cgaaggtcca gcgccgcccg
      481 cccgttccgc ccaatccgac gcttccgaga ttgcaagacg ccgcccgcaa ttgccgcgct
      541 tgcgatttat ggcgcaatgc cactcaaacc gtattcggcg ccggaccgcc gcgcagcaaa
      601 atcatgctgg tcggagaaca gcccgggaat gatgaggacc tcgacggccg cccgttcgtt
      661 ggaccggccg ggaaacttct cgaccgcgcc ctgattgaag cgggaattga tcgaaccgaa
      721 gtttacgtca ccaatgccgt aaagcacttc aaatgggagc cgcgaggcaa gcgccgcatt
      781 cacaaaaagc cgaattccgc ggagattgcg gcatgccggc catggcttga cgccgaaatt
      841 gccgtcgtgc gtcctcaagt gataatttgt atgggagcca ccgccgcgca atccttgttt
      901 gggcgcgagt ttcgcgtgac caagcaccgc ggtgagtttc tggaatcctc gctcgctccc
      961 accgtgacgg ccacgattca tccttccgca atcctgcggg cacccgatga agaaacgcgc
     1021 cgcatggaat tcgcaaagtt cgtagccgac ctgaaaaaag cttcgcagcg gcactgaaac
     1081 ttcatttgcg cgtttttcgg gcgatcagtt cccgagatga ttgtccgcgc ttgccatggg
     1141 cacgtcttcg cgcagagcgc ttaggaattc gcggggatgc gtccacatca cgaaatgccc
     1201 ggcatcgggt acgatatcaa gtttctttgc cggcgcctgg atcttcgcag cataatctgc
     1261 cactaggctg gtaggtgcaa cattgtcgtt ctcaccctgt atgaaaaatg ccggcacgga
     1321 aaacttcgtg tccgtgtcgc gcaggtcagc catattgaca gcgatggcgt ccagctttac
     1381 ctcgtccgtt cgcagcgcct tgtaccaccc gtacgcctgg gagatcgata ggcttggcgt
     1441 gaccacgagc aaacctctca ggcggttgat ccctgcaatg tcatcaggcg gccagaatgg
     1501 attgcggcaa cagtcttgaa accgtccgta gcgctcaaga gtgctgtagg gcggagggcc
     1561 cacctgcttg agcgcagcca acatcttttc atcacggttc ccggttgcgt ggctcagtgc
     1621 gtatcgatat agcacaacct gtcgcgcctg atcaccgacg accagtcccg tcccaacgag
     1681 caacttgaat aggtccggtc ttttctcgat catgcggatg ccgaccaccg ctccccaatc
     1741 ctggccaatc aggatgatct ttggcgcgtg cagctcggtc cgcaggtact cggcaaccgc
     1801 gatcccgtcg cttacggcac cgtccagcgt gagcttcggc tggtggtcgc ccgccttgat
     1861 aaacgtcatt ccggcgccgg gttgatccca ttgcacgaaa acgtagttct cctcgaaagg
     1921 taagtagagc tcggcgaaag gggagttcgc ttcggcgggg cctccgtgca gaaatagaat
     1981 caccggatta tcgcggtcct gcccgcgaat ggtgatccat tcctgcgcgc cattaatctc
     2041 gacgaacttc gcttcgttaa tgccgtttcg cgagttgatc ttcatctcat ttgcgatgtg
     2101 gtgttgccgc caagctcggt aacaaaggcc cccggccgcc gccagaacaa ctactgcagc
     2161 cagcactaca aatattctgc gtatcactcg aatcacgggc gcggtttttc cgcgagcacc
     2221 agtggcgagg gcccgggcgc ctcttgcaat cgcgcgttca caaagccggc ctcctcgagc
     2281 cacgaacgaa tctcggcgaa cgtgtatgca ttcccgttct tcgtatggag cagcatgttg
     2341 atggcaaatg tcagcgggac cagcggccca ttgcggtcgt cgtccacaag gaactctccg
     2401 attgcgatcg ttcctccggg agcgagcgcg tcaaacgttt tcttcagcag cgttttgttt
     2461 tccgtcggac ccagcatgtg caggatattt cccagcgtcg cgatagaatg gtccttcccg
     2521 taatccactt taaatagatc gccgccaaga tcgtggaatc gctcggcgac gccgtatcgt
     2581 cccgcaattc gccgcgtcac cgtcagcaca ctgggccaat ccacggccgt cacgctgacc
     2641 tgcggcgaag cctgcgccag cgcgatgccc cacgcgcccg aacccgatgc caggtcgaga
     2701 accgagatgg gctgcgtcgc gcgcgaaagg cccagcacct cggccagctt ctgcgccgga
     2761 ggaaacgcca gcggcatgat gtccaccacg agctcttcaa aaaaggcagc tgcttccggt
     2821 ctctcgctct cttcgccttc cggaggcgta cccgtgcgca tcacctcagg caactggaga
     2881 aagccttcgg cgagccgcgt gccaacctga cgaataaagc cgcccatgta ccctggcttc
     2941 gagctaacca aaaaagcagc gctttcagga gtcagcgagt agcggccgtc ggacaacttc
     3001 gccagcaaat tgatgtgagc gagcaagttc atcaccgcgg taaggccgcg caccgaggcg
     3061 cctgtctctt tgctgatttc ggccacggac ttcggtccag agtccagcac atcgaacacg
     3121 cgattctgta tggcgctgcc aattgccagc ggctgcgcga agctccacgt gatttgcatc
     3181 agccgctccg gattcacgtg cgtttgaggt tgtgatccca tttctcggtc ctctcctttt
//
LOCUS       DATNDT010000074        11874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3505543, whole genome shotgun sequence.
ACCESSION   DATNDT010000074 DATNDT010000000
VERSION     DATNDT010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11874
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505543"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..93)
                     /locus_tag="VN788_02150"
     CDS             complement(<1..93)
                     /locus_tag="VN788_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013296623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HXU19366.1"
                     /translation="MPDFAPSITRLIDELRRLPGIGQKTAQRLAF"
     gene            505..954
                     /gene="rplM"
                     /locus_tag="VN788_02155"
     CDS             505..954
                     /gene="rplM"
                     /locus_tag="VN788_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HXU19367.1"
                     /translation="MRTYVPKGREAEALTVGANWFVIDANGQVLGRLATRVARLLIGK
                     DKPSYTPYLDSGDHVVVINAERIKLTGNKIDQKIYRHHSGYPGGLKEIPIRLMMQRRP
                     EEVVREAVLGMLPKNKLRARRAKKLRVYAGNEALARHAGQKPQLLAQ"
     gene            1063..1485
                     /gene="rpsI"
                     /locus_tag="VN788_02160"
     CDS             1063..1485
                     /gene="rpsI"
                     /locus_tag="VN788_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HXU19368.1"
                     /translation="MSSTPNSVADAAGKQFYGTGRRREAVARVYIKVGPARFTVNGRP
                     VDEYFRNIAWNTAAVEPLKFTQMHDQVEVKAQVKGGGVGGQAGAVRMGIARALSKLNP
                     ELRPALRKNGFLTRDPRMKERKKYGQKGARRRFQFSKR"
     gene            1602..2459
                     /gene="rpsB"
                     /locus_tag="VN788_02165"
     CDS             1602..2459
                     /gene="rpsB"
                     /locus_tag="VN788_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HXU19369.1"
                     /translation="MKELLEAGVHFGHQTRRWNPKMKEYIFGERNGIHIIDLQKTLKM
                     FRDAARYVSEHAAQGKNVLFLGTKRQAQEAIAEEAQRCGMFYVNHRWLGGTLTNWATL
                     QKSIKRFKLLKAMSEDGRMAEFSKKEGARLDRELKHLRQNFCGVENMTTLPDTIFVID
                     PNAEVIAVHEARRMGIPVVAIVDTNCDPSLVDWVIPGNDDALRAIRLFTSKISDAVLA
                     GRQSFEQSQIADEKTAEGAPGEEGPEYVDTSAYEQYEKQEGDFVEGEPSSTEEVPTPV
                     TGEDETPTK"
     gene            2536..3216
                     /gene="tsf"
                     /locus_tag="VN788_02170"
     CDS             2536..3216
                     /gene="tsf"
                     /locus_tag="VN788_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HXU19370.1"
                     /translation="MATATEPGKGNPIPAQLVKELRDKTGAGFADCRAALIEVNGDIE
                     QAIGILRKKGQMAAAKKAQRATSEGLVSSYIHAGGKIGVLLETNCESDFVARTEDFQS
                     LCHDVAMHVAALDPQFLRREEVTQEMLDQKRKLYSEEAKASGKPEPVIEKIVNGKMEK
                     FYEENCLLEQHFIKDEGITVKELVDQAIAKLGENISIRRFSRFKVGETDGAGASAGSP
                     EPVAGPSV"
     gene            3346..4122
                     /gene="pyrH"
                     /locus_tag="VN788_02175"
     CDS             3346..4122
                     /gene="pyrH"
                     /locus_tag="VN788_02175"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HXU19371.1"
                     /translation="MASKTIVKKVRRVVYGSDRAGKPAFQRIVLKLSGESLSGPDAFG
                     IHAETIQNIAREVKEVHDLGIDVALIVGGGNIFRGARQHGLTIDRATGDYMGMLATVL
                     NGLALQDALEKAGCHTRLMSAIEMHQVAEPFIRRRATRHLEKHRVVIFAAGTGNPYFS
                     TDTAAALRAMEIKAQVILKATRVDGIYDADPEKVANAMMFDQISYLDVLQKGLAVMDS
                     TAISLCMDNKMPIVVFNMNVRGNLRRVVMGERVGSTVTAA"
     gene            4151..4726
                     /gene="frr"
                     /locus_tag="VN788_02180"
     CDS             4151..4726
                     /gene="frr"
                     /locus_tag="VN788_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HXU19372.1"
                     /translation="MTTIATSKDALAQAKTRMEKAVEDFRKELSGVRTGRANVSLLDH
                     VRVDYHGTPMPINQLATLNVPDPASIVIAPWDAGALPLIDKAIRTSDLGLNPANDGKV
                     LRVPIPPLTEERRKDLVKHIHKVLENHRTAVRNIRRDLKEAVEKLEKEKKISEDEKKR
                     SLDELEKLTHSETKKIEDLSATKEKEVMEIK"
     gene            4900..5724
                     /locus_tag="VN788_02185"
     CDS             4900..5724
                     /locus_tag="VN788_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU19373.1"
                     /translation="MHVAIRRAAHQIFDRPPVFEDPLAVPIVGLEAAARLASEEKRYG
                     QRFGRSIRAFMAARSRYAEDQLALAVARGARQYVILGAGLDTFAYRNPYPKEALRVFE
                     VDHPATQEWKRQQLAKASIPIPPSVIHAPVDFERQTLADGLAKAGFDIEAATFFSWLG
                     VVMYLTEGVTMSTLGFIASTPPGGGVVFDFAVPRESLNWMGRLALDAISRRVAAAGEP
                     FRTFFDPHVLSEKLATIGFHAVETLGAEEINARYFTGRDDELRVSGNVGRLMKAQV"
     gene            complement(5804..9016)
                     /locus_tag="VN788_02190"
     CDS             complement(5804..9016)
                     /locus_tag="VN788_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HXU19374.1"
                     /translation="MNRLFLALICLSILPVPTLAQKRLAPARGSESKTPISTEKGPDH
                     SREPLVIEQYFSTARFENDGTGERDIAVKARVQNDAGAKQLSQIVFPYNSASEQIDVR
                     YVRIRKSDGKITTAGQDAIKELTAPIARNAPAYSDCKEKQISLPALAPGDTLEYEIST
                     RIVKPAAPGQFWFEHRFLKDSIVLDERLEVSVPASRKIVLKSPAISRYQTETANGRIT
                     YRWERSNLAHAPAGSSNKEPEQQVENPPDVQFSTFASWQDLAGWYTKLAGRAESSPEV
                     RVKTQELTQSSTSQIEKIQAIYDFVSKNIRYVDIPFGTGPYQPHRAAEILANGYADSN
                     DEHVLLAAMLDAVGIRSEAAFVSYRRELDASLPSPAQFDHLVTAVPLRSEVVWMDPTP
                     GVAPFRLLASPLRDKSALLVSAEGDGQIVKTPADPPFVSTQNVHIDGEVSDLGKLTAH
                     AHYTLRGDTEFVLRLAFHKTPEAQWTQLGQTILSLDGIQGEVISVKPSDPVDTKDPFA
                     LDIAFTQSNLVDWSAKRAKAELPLLAIGVPSAPAKSSQPIHLGSPLKVNVSLKLALPA
                     LFAAQPPVGTSLTRDYAEYKSSYRYAEHTVTAQRSLDFKMRELPASRADDYDAFTRAV
                     TKDETQPLSIENTTAGAPTVPASAAADELLEAGLAAFNAGNAASAIPLFERLVQIDPR
                     HKSAWNDLGLARLRVGNYDAAITAFQKQLEVNPSDQHANDYLGLAYQQQRNYPEAIAA
                     FHKQITANPLDAVAHATLGEILLAQNEYAAAATELDKAAILSPENVGLRLALGRASLN
                     SGDDKKALAAFEAAAQLSPTPPVWNNIAYNLADRAADLDKAQHYAESAISATEADLQK
                     IDLSHLTPEHLSDVANLGAYWDTLGWVYFRKVDLASAERYVRAAWMLDQSGEIADHLA
                     QIDEQLGQKARAIHEYALSLATPGSLPETRAKLILLLGGNTQIDALVEQARPDLAALR
                     RIPAGKLDAEDIQADFLILLSPGEKSARVEDVRFIGGSDRLKPFSDRLRSLNYGVVFP
                     NHTPVKLVRRATLACAAKTGACTLTLIRPEDLAAAYKSVK"
     gene            9736..10119
                     /gene="rpsL"
                     /locus_tag="VN788_02195"
     CDS             9736..10119
                     /gene="rpsL"
                     /locus_tag="VN788_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXU19375.1"
                     /translation="MPTFSQLVRSGREQIRAKTKSPALDGCPEKRGVCIRVYTQTPKK
                     PNSALRKVARARLTNHAEVTTYIPGIGHNLQEHSIVLIRGGRVKDLPGVRYHVIRGTL
                     DAAGVDNRKQSRSKYGAKRPKSAQK"
     gene            10132..10602
                     /gene="rpsG"
                     /locus_tag="VN788_02200"
     CDS             10132..10602
                     /gene="rpsG"
                     /locus_tag="VN788_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXU19376.1"
                     /translation="MPRKGWVLRRKIPGDPVYGSDLVQKFISSMMYDGKRSTAQTIFY
                     DAMDQVAKKTNDDAFKLFKKAIENCKPMLEVKSRRVGGANYQVPVEVNPFRKQSLAIH
                     WLVLYSRTRSGKSMTDKLAEEIMDAANGRGGAVKKKEDVHRMADANKAFAHYRW"
     gene            10619..>11874
                     /locus_tag="VN788_02205"
     CDS             10619..>11874
                     /locus_tag="VN788_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011365698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXU19377.1"
                     /translation="MARQVQLGQIRNIGIMAHIDAGKTTCTERILFYTGVTYKIGEVH
                     EGTTVMDWMEQERERGITITSAATTASWKRFDKSYRINIIDTPGHVDFTVEVERSLRV
                     LDGAVAVFDAVAGVQPQSETVWRQADKYRVPRIAFINKMDRIGADFDHSIRSMRQRLS
                     AHPVPVTYPIGREDNFVGIIDFIEERAIVWKDETLGAEYEVLPLEKLWDAEYLKTRPA
                     LETAVKATALSPEFYKEHREKVVEYIAEHDDAILTKYLEQHKLEPAELRASLRRSTIA
                     LKLVPVIAGSAFKNKGVQPLLDAVIDYLPSPVDVPPVEGTTPDGTEPELRRAADDQPF
                     AALAFKIMTDPFVGHLTYIRVYSGVLKTGQSVYNSTRKTRERMGRLLRMHANKREEIE
                     SIEAGDIAACVGLKNATTGDTLCDEE"
BASE COUNT         2705 a         3057 c         3693 g         2419 t
ORIGIN      
        1 aaacgccaga cgctgcgcgg ttttttggcc gataccgggc aaccgtcgaa gttcatcgat
       61 cagtcgtgta atggaaggcg cgaaatcagg catgcgcgct cattattgca aagtggcccg
      121 atctccgcca gtgtgctgcc tatgtgccgc agcgaagtcg atccgcgaac cgatctcacg
      181 ggtccttcgc caacggcgag attcgtagga tttctccggc gcgtctgagt tcaaccggcc
      241 attgggcatt tcttcgcatt ctaatcgacg cttcagccga gtataatgcg acccaactta
      301 cagaaaacaa gggacggcga ctgactctac tcgacacttt tcgaaaaggg tccatcgacg
      361 tctgccttcg atggcgtctt caatatctgg cgagtccgcg cttcgacatc gcctcacgag
      421 ccgctgcgca gtcttcgttg acagaaattg ctccgtagcc tacactttgc gtttgccttg
      481 acagggaaag gtgcgaccgg actgatgcgg acatatgttc caaagggccg tgaggcggag
      541 gctctcacgg taggggctaa ctggtttgtg attgacgcga atgggcaggt gctggggcgc
      601 ctggcaacgc gcgtcgcgag gctattgatt ggcaaggata aaccgagcta cacgccatac
      661 ctggattcgg gcgatcatgt ggttgtcatc aatgcggagc ggatcaagct gacgggcaac
      721 aagattgacc agaagattta tcggcatcac agtggttacc cgggtgggct gaaggaaatt
      781 ccgattcggt tgatgatgca acgccgcccg gaagaggtcg ttcgcgaagc ggtgctgggg
      841 atgctgccga aaaacaaact tcgcgcgcgc cgtgccaaga agttgcgtgt ttacgccggg
      901 aatgaagcgc ttgcccgcca cgccggacag aagcctcagc tgctcgcgca gtaacctcgc
      961 gaactgagtt tcgaaatttc aagcgccccg tgcatcggtt cgtcgcaggg gttgcgttga
     1021 ggctggcaca ataacgtgcc tagtcaagaa agaggaggac agttgagttc aacgcctaat
     1081 tcggtcgccg atgcggctgg gaaacaattc tatggtacgg gacgccgccg cgaagcagtg
     1141 gcgcgggtct acattaaagt tgggcctgcg cgattcacgg tgaatggccg gccagtggac
     1201 gaatattttc gtaatatcgc ctggaacacg gccgcggttg agccgctgaa atttacgcag
     1261 atgcacgacc aggtcgaggt gaaagcgcag gtgaagggcg gcggagtggg cggacaggcg
     1321 ggggcggtgc gcatgggcat agcccgggcg ctctcgaagc tgaatccgga gttgcgtccc
     1381 gcgctgcgaa aaaacggttt cctcacgcgc gatccgcgca tgaaggaacg caagaagtat
     1441 gggcagaaag gcgcacgccg ccgcttccag ttcagcaagc ggtagtgggt gcctgattaa
     1501 attcacggcc ggcaaggaac cggcagaaaa ggatgctgtc ccggtaatcg ggatcgaagc
     1561 atcccttcaa tagaggaggt actttgggag ttgaaattac gatgaaggag cttctggaag
     1621 ccggagttca cttcggccac cagacgcgcc gctggaatcc gaaaatgaag gagtacattt
     1681 tcggcgaacg caatggaatc cacattatcg atctgcagaa gaccttgaaa atgttccgcg
     1741 acgcagcgcg ttacgtttcc gaacatgccg cgcagggaaa aaacgttctc tttttgggga
     1801 ccaagcgcca ggcgcaagaa gccatcgccg aggaggcgca gcgctgcgga atgttttacg
     1861 tgaatcaccg ctggcttggc ggcacgctca cgaactgggc gacgcttcaa aaatccatca
     1921 agcgattcaa gctgctgaaa gccatgtccg aagacggccg tatggccgaa ttttcgaaaa
     1981 aagagggcgc gcggctggac cgcgagctaa aacatttgcg gcagaatttt tgcggcgtcg
     2041 aaaacatgac cacgctgccg gacacaattt tcgtgatcga tccgaacgcg gaagtcatcg
     2101 cggtgcacga agcccggaga atgggtattc ccgtggttgc gatcgtggac acgaactgcg
     2161 atccgagcct tgtggattgg gtgattcccg gcaacgatga cgcgctacgg gcgattcgtt
     2221 tgttcacctc gaaaatttcg gacgcggtgc tcgccggcag gcaatcgttc gagcaatcgc
     2281 aaatcgcgga tgaaaagacg gccgagggag caccaggcga ggaagggccg gagtatgtgg
     2341 acaccagcgc ctacgagcag tacgagaaac aagaaggcga ttttgtggaa ggcgaaccgt
     2401 cttcaacgga agaagttccc actcctgtaa cgggcgaaga tgaaactccc acgaagtagc
     2461 gcgggaaggg tttaggaaac cgtcggcttg ccgcatccca gcaggccaaa cccagataag
     2521 gaccgagcag atcccatggc gacagcaaca gaacccggca agggcaaccc aatacccgcg
     2581 caactcgtga aggagttgcg cgacaaaacc ggcgccggtt tcgccgactg ccgcgcggcg
     2641 ctgattgaag ttaacgggga tatcgagcaa gccatcggca tcctgcgcaa gaagggacag
     2701 atggccgcgg cgaaaaaggc ccagcgggcg acttccgagg gattggtttc cagctatatt
     2761 cacgccggag ggaaaatcgg cgtgctcctg gagaccaatt gcgagagcga cttcgtagcg
     2821 cggaccgagg attttcagag tctctgccat gacgtggcca tgcatgttgc cgcgctcgat
     2881 ccgcaattcc tgcggcgcga ggaagtgacg caggaaatgc tcgaccagaa gcgaaaactt
     2941 tatagcgagg aagcgaaggc cagcggcaag cccgaaccgg tgatcgaaaa aatcgtcaac
     3001 ggaaagatgg aaaagttcta cgaagaaaat tgccttctcg aacagcattt catcaaggac
     3061 gaaggaatca ccgtgaaaga gttggtggac caggccatcg cgaagcttgg tgaaaacatc
     3121 tcgattcgtc gcttttctcg gttcaaggta ggcgaaacgg acggggctgg agcctcagcg
     3181 gggagtccag agccggtggc aggaccgtcg gtgtagcatc ttttattcgg cgtgaccgcg
     3241 tacgttcctc cgttgtggaa aagataaagc ctcccgagcc gtgaaatcct ttcgtccaac
     3301 gacctctcat cgaacagcct attctctgct agaatgccgc gagccatggc gtccaaaacg
     3361 attgtgaaga aggtgcgaag ggtcgtgtac ggctccgacc gggcagggaa acccgctttc
     3421 caacgaatcg tgctgaaact ttcgggcgaa tcgctatcgg gaccggacgc tttcggcatc
     3481 catgcggaga caatccaaaa tatcgctaga gaagtaaaag aagtgcacga tctgggaatc
     3541 gatgtagcgc ttattgtcgg cggcggaaat atttttcgcg gggcccggca gcatggcctc
     3601 accatcgatc gcgccaccgg agactacatg gggatgctgg cgaccgttct gaacggactg
     3661 gcattacagg acgcgctgga aaaagccgga tgccatacgc gcttgatgag cgctatcgaa
     3721 atgcatcagg tggcagagcc cttcatccgc aggcgagcca cgcgccacct ggaaaagcac
     3781 cgcgtggtga ttttcgccgc gggtaccggg aacccctatt tttcaacgga cacggcggcc
     3841 gcccttcgcg cgatggaaat aaaagcgcag gtgatcctaa aggccacccg agtggacgga
     3901 atctatgacg ctgatccgga gaaagtggca aatgcgatga tgttcgatca aatcagctat
     3961 ctggacgtct tgcagaaagg cctcgcggtg atggattcca cggctatttc actgtgcatg
     4021 gacaacaaaa tgcccatcgt ggttttcaat atgaacgttc gtggaaattt gaggcgcgtg
     4081 gtaatgggcg agcgggtcgg ttcgaccgtc actgcggcat gaaactgcaa acgcggagga
     4141 gaacttttcg atgaccacga ttgccacgag caaggatgct cttgcgcagg caaaaactcg
     4201 aatggagaaa gcggtcgagg atttccgcaa ggagctttca ggcgtgcgca ccggccgggc
     4261 gaacgtgtct ttgctcgatc acgtgcgcgt cgattaccac gggacgccga tgccgatcaa
     4321 tcagctggcc acgctcaatg ttcccgaccc agcaagcatc gtgatcgcgc cctgggatgc
     4381 cggagcgctt cccctgatcg acaaagctat acgcacgtcc gacctgggac tgaatccggc
     4441 gaatgacgga aaggtgctgc gtgtcccaat tccgcctctg actgaagagc gccgcaaaga
     4501 tttggtgaag catattcaca aagtgctcga aaatcatcga acggcggtgc ggaatatccg
     4561 gcgcgacttg aaagaagcgg tggaaaaact cgagaaagag aaaaagatca gcgaggatga
     4621 aaagaagcgg tcgctcgatg agcttgagaa acttacccac tccgaaacca agaagatcga
     4681 agacctttcc gccacgaaag aaaaggaagt aatggaaatt aagtaagagt ttctctgtgg
     4741 cagtacgaag agagattcct catctgcgtg tcctggcgct tcgcgcgaaa ccaaagggca
     4801 cttcgctccc ttcctgcgtc agggcaagcg gaatgacggt tttggctgcc tatcggcgat
     4861 ggctcctttg tgattgaggc gcggcctagc aggacagcga tgcatgttgc aatacggcgc
     4921 gccgcgcatc agatttttga tcgtccacct gtgtttgaag atcccttggc ggttccgatc
     4981 gttgggctcg aagcagcggc aaggctcgcg agcgaggaga agcgatacgg gcaacgcttc
     5041 gggcgaagca ttcgcgcgtt catggcggcg cgcagtcgtt acgcggaaga tcaactcgcg
     5101 ctggcggtcg cgcggggagc acggcaatac gtgattctcg gggccgggct ggacaccttc
     5161 gcttatcgca atccttaccc caaagaggca cttcgcgttt tcgaagtgga tcacccagcg
     5221 acgcaagaat ggaagcgcca gcaactggcg aaagcctcga ttccaattcc cccgtccgtc
     5281 atacatgcgc ctgtagattt cgagcggcag acgttagcgg atggattggc aaaagccgga
     5341 ttcgacattg aagcggcgac atttttctcg tggctcggcg tcgtaatgta ccttaccgag
     5401 ggagtgacga tgtccacttt gggcttcatc gcttcgactc cgcccggagg gggcgtggtc
     5461 ttcgactttg ctgttccccg cgaatcgctg aactggatgg gacgcctagc gctggatgcc
     5521 atctcgcggc gtgtggccgc cgcaggagag cctttccgca cattttttga tccgcacgtc
     5581 ctgagcgaaa agctcgcgac aattggattt catgccgtgg aaactttggg tgcggaagag
     5641 atcaatgcgc gttacttcac aggtcgcgac gacgagttac gagtttcggg caacgtcggc
     5701 cggttgatga aagctcaggt gtagcgaagg cgacgggcga aggaagagat ggccgggagc
     5761 cgcagcgctt ttctttcagg ccgcgcgttc aacgagtacg cgcctatttt accgacttgt
     5821 acgcggcagc gagatcttcc gggcgaatca gcgtcaaagt gcaagcacct gttttcgcag
     5881 cgcaggccag cgtggcgcgg cgaacgagtt ttaccggcgt gtgattcgga aacaccactc
     5941 cgtaatttaa cgaacgcagg cgatcggaga atggcttgag cctgtcactt cccccgatga
     6001 atcttacatc ctcgactcgc gcggattttt cgccaggcga cagcagaatc agaaaatcag
     6061 cttgaatgtc ttcggcgtcg agttttcctg cgggaatccg gcgaagcgcg gcgagatccg
     6121 gccgggcttg ttcgacgaga gcatcgattt gtgtgttgcc cccgagcagc aaaatcaatt
     6181 tggcgcgagt ctccggaaga gagccgggag tcgcgagaga aagcgcatac tcgtgaatgg
     6241 cgcgagcttt ctgtcccagt tgttcatcga tttgcgccag gtggtcggcg atctcgccag
     6301 attggtcgag catccaggcg gcacgaacgt aacgctcggc tgatgcgaga tcgacctttc
     6361 gaaaataaac ccaaccgagc gtgtcccaat aagcgcccag gttagcgaca tcgctaagat
     6421 gttcgggcgt taagtgcgat aagtcgattt tttggaggtc cgcttcggtg gcggagatcg
     6481 cggattcggc gtaatgctgc gctttgtcga ggtctgccgc ccgatcggcg agattgtagg
     6541 cgatgttgtt ccacacgggt ggcgtgggcg agagttgcgc ggcggcttcg aaggcggcga
     6601 gcgctttttt gtcatcgcct gaattgagag aggcgcgccc taaagcgagc cggagcccga
     6661 cattttccgg agataaaata gcggccttgt cgagttcagt tgcagcggcg gcgtattcat
     6721 tttgtgcgag cagaatttcg ccgagtgtgg cgtgggccac ggcgtcgagc ggattcgctg
     6781 tgatctgttt gtggaaggca gcaatcgctt ccggataatt tcgctgttgt tggtaggcga
     6841 ggccgaggta gtcattcgcg tgctggtcag agggattcac ttccagctgc ttttggaacg
     6901 cggtgatcgc tgcgtcgtaa tttccgacgc gaaggcgggc aagccctaaa tcgttccacg
     6961 cacttttgtg gcgtggatct atttggacga gacgttcgaa gagcgggatt gcagaggcgg
     7021 cgttgccggc attaaaagcg gcaaggccag cttcgaggag ttcgtccgca gccgccgatg
     7081 cgggaacagt gggagcgccc gcggttgtat tctcgataga cagcggctgt gtttcgtcct
     7141 tggttaccgc ccgagtaaaa gcatcataat cgtcggcgcg cgaagcgggc agttctcgca
     7201 ttttgaagtc gagggaacgc tgcgccgtga ctgtgtgttc cgcgtagcga tagctcgatt
     7261 tgtattcagc gtagtctcgg gtgagagacg ttcccacggg cggctgagcc gcgaaaagcg
     7321 cggggagcgc aagtttcaga ctgacattga ctttgagcgg gctgcccaga tgaatcggct
     7381 gggaggattt ggcaggcgca ctaggaacgc cgattgcgag cagtggcaat tcagccttcg
     7441 ctcgcttcgc agaccagtca acgaggttag attgagtgaa ggcgatatcg agcgcaaaag
     7501 gatccttggt atccacggga tcacttggtt tcacggagat tacttcgcct tgaatgccat
     7561 ccagagacag aatggtctgc ccaagttgtg tccattgtgc ttcgggcgtt ttgtgaaagg
     7621 cgaggcgcag cacgaactcg gtgtctcctc gcaaagtgta gtgcgcgtgc gctgtgagtt
     7681 tgcctaaatc gcttacctcg ccatcaatgt gaacgttttg ggtagagacg aaaggcggat
     7741 cggcgggagt tttgacgatc tgaccgtcgc cttctgccga cacgagaagc gcggacttat
     7801 cgcgcaatgg cgaagccaga agcctgaatg gcgccacgcc gggtgttgga tccatccaaa
     7861 caacttcgct gcgaagcgga acggctgtga ccaggtgatc gaattgtgcc ggggagggaa
     7921 gcgaggcatc cagttcgcgc ctgtagctga cgaatgcggc ctccgaacgg attccaacgg
     7981 catcgagcat cgcggcgaga agcacgtgct cgtcattcga atcggcgtaa ccattcgcaa
     8041 gaatttcggc cgctcgatgc ggctggtaag gtcctgtgcc aaaaggaatg tccacatagc
     8101 gaatgttttt cgatacgaaa tcgtagattg cttggatttt ttcgatttgg ctcgtgctcg
     8161 attgagtaag ttcctgggtt ttcacacgga cttccggcga ggattcagcg cggccggcga
     8221 gcttcgtgta ccagccggct aagtcctgcc aggatgcgaa agtagagaac tggacatccg
     8281 gaggattttc gacctgctgt tctggttcct tgtttgaaga accagcgggt gcgtgggcaa
     8341 gattcgatcg ttcccagcgg taagtgatgc gaccatttgc ggtctctgtt tggtagcggg
     8401 agatcgccgg cgatttcagt acaatttttc tggaggcagg gacgctgact tcgaggcgct
     8461 cgtcgagaac gatcgagtcc ttgaggaaac ggtgctcgaa ccagaattgg ccgggtgccg
     8521 cgggtttgac aatccgcgtg gaaatttcgt attcgagggt gtctccgggc gccagagctg
     8581 gcagcgagat ttgtttttct ttgcaatcag agtaggcggg ggcgttgcgc gcgatgggcg
     8641 cagtcagttc tttgatggcg tcctggccgg cagttgtgat cttgccgtct gattttcgga
     8701 tgcggacgta acgaacgtca atttgctcgc tcgcggaatt ataggggaat acaatttggc
     8761 tcaactgctt cgcgcctgca tcgttttgaa cgcgcgcttt cacggcaatg tcgcgctcgc
     8821 cggtcccgtc attttcaaag cgagcagtgc tgaaatattg ctcgatcact aaaggctcgc
     8881 gagaatgatc gggtcccttc tccgtagaga tcggcgtctt ggattccgag cctcgcgctg
     8941 gcgcgagacg tttttgggca agcgtgggta ccggcaggat tgagaggcaa atgagggcga
     9001 ggaaaagacg attcatccat gctccctgtt gaaatctttc gccatgagtt taggtgcgtt
     9061 tgcgggcatc ggtaaagcag ggatcgggga tcgggtgaga aattggcaat gggtgtccga
     9121 aagctagaag cataggacaa cgcaccgcgc caggccgcaa acatcctttt gcgatcctaa
     9181 ccccgcttgt aagtcttgta tcgtctagcg gatacgggat tttaccgtgt aggggcccgc
     9241 tcctaaagcg ctcaggactc gcgatccgat aggcataacg aaaaatggga cggaacgcgt
     9301 ttgccgcgga aatgacccct cgggttgagg accgggagcc gcagggaata aagagcggaa
     9361 gtcttttggt tgcatatttc cacagaagta tcgtgttgac acaatttgtt gttttaccta
     9421 tactcttgcg gttgtcttca ggcgattttg gcctggtggt gaaagggaag tcttcgcacc
     9481 ccggaacgca gaaaaaagga acggggtgaa gaagagcggc cgaaatgggg cgccagcccc
     9541 gcaaagccgt gcgagcctct ccgcagtgta agatgcagtt cactccttgg ggtgaagcga
     9601 agcgtcagcg gagtacctgg cagttccttc aggtttggcg accttttctg ttcatgcact
     9661 cgcagaattt cgcgcgagcg gcacttgtgc tgcgcgaaat cgcaacaaca aacggcttgg
     9721 ggagcggggg gaagagtgcc gacgttttca caattggtgc gcagtggccg cgaacaaatc
     9781 cgagcgaaaa ccaagtcgcc ggctctcgac ggatgtccag agaagcgcgg ggtgtgcatc
     9841 cgcgtttaca cgcagacgcc gaagaagccg aactcggcgc tgcgaaaagt ggcgcgcgcg
     9901 cggctcacca atcacgctga agtgaccacc tacattcctg ggatcgggca caacttgcag
     9961 gaacactcga tcgtgctgat tcgcggtgga cgcgtgaagg atttgccggg cgtgcgctat
    10021 cacgtgattc gcggaacgct cgatgcggcg ggcgtcgata atcgcaagca atcgcgctcc
    10081 aaatatgggg cgaaacggcc caaatcagcg cagaagtagg agaacggaaa gatgccacga
    10141 aaaggatggg ttcttcggcg caagattccg ggcgatccgg tgtacggaag cgacctggtg
    10201 caaaagttca tttcgagcat gatgtacgac ggcaagcgct ccaccgcgca gacgattttc
    10261 tacgacgcga tggatcaagt agccaagaaa acgaacgacg acgcgttcaa gcttttcaag
    10321 aaggctatcg aaaattgcaa gccgatgctg gaagtaaagt cgaggcgcgt cggcggagcg
    10381 aactaccagg tgccggtgga agtgaatccg ttccggaagc aatcgctcgc gattcactgg
    10441 ctggtgcttt actcacgcac gcgttcgggc aagtcgatga ccgacaagct ggctgaggaa
    10501 attatggacg ccgcgaatgg ccgtggcgga gcggtaaaga agaaagagga cgttcaccgc
    10561 atggcggatg cgaacaaagc gttcgcccat tatcggtggt aacggatcga ttctaatcat
    10621 ggcgcgacaa gtacaactcg gccagattcg caacatcggg attatggcgc atatcgatgc
    10681 gggaaagaca acctgcaccg agcgcatcct gttttataca ggtgtgactt acaaaatcgg
    10741 cgaagtgcac gaaggcacca cggtgatgga ttggatggag caggagcgcg agcgcgggat
    10801 caccattacg tccgccgcga cgacggcttc ctggaagcga ttcgacaaat cctatcgcat
    10861 taacatcatc gatactccgg gccacgtcga tttcaccgtg gaagtggagc gttcgctgcg
    10921 cgtgctggat ggcgcggttg cggtattcga tgccgtggcc ggcgtgcagc cgcaatcgga
    10981 aacggtgtgg cggcaagccg acaagtatcg cgtgccgcgc attgcgttca ttaacaagat
    11041 ggaccgcatc ggcgccgact tcgatcattc cattcgctca atgcggcaaa ggctttccgc
    11101 gcatcccgtt cccgtcactt atcccatagg ccgcgaagac aatttcgtgg gcatcatcga
    11161 ttttatcgag gagcgcgcca tcgtctggaa ggacgaaacg ctgggcgcgg agtacgaagt
    11221 tcttccgctc gaaaaacttt gggacgcgga gtatctgaag acgcgtcctg cgctggaaac
    11281 tgcggtgaag gcgacggcgc taagccccga gttttacaaa gagcaccgcg aaaaagtcgt
    11341 ggagtacatc gccgagcatg acgatgcgat cctcacaaaa tatctcgagc agcataagct
    11401 cgagcctgcc gagctccgcg cttcgctgcg ccgctcaaca attgcgctga agctggtgcc
    11461 tgtgatcgcc gggtccgcgt ttaagaacaa gggagttcag ccgctgctcg atgcggttat
    11521 cgattatctg ccttcgccgg ttgacgtgcc tcctgtggaa ggcaccacgc ctgatggcac
    11581 ggagccggaa cttcgacgag ccgcggacga tcagccattt gccgcgctgg ccttcaagat
    11641 tatgaccgac cccttcgtgg gccatctgac ctacatccgc gtgtactcgg gcgtgctgaa
    11701 gacgggtcaa tcggtttaca actcgacgcg aaaaactcgc gagcgcatgg ggcgcttgct
    11761 gcggatgcac gcgaataagc gcgaggaaat cgagtcgatt gaagcgggcg acatcgccgc
    11821 ttgtgtgggc ctgaagaacg cgacgaccgg cgacacgctc tgcgatgaag agaa
//
LOCUS       DATNDT010000075        12351 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4054440, whole genome shotgun sequence.
ACCESSION   DATNDT010000075 DATNDT010000000
VERSION     DATNDT010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12351
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4054440"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VN788_02210"
     CDS             <1..487
                     /locus_tag="VN788_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXU19378.1"
                     /translation="RQAKTEYFDIMLLHFQHSGTWPVDTVAWQDGIEEAEHKKAVLSR
                     GASVHGLPALRQVPGDSWLDVAMIRMNHKGVHMDAEDYATDGLGNVPEVVKHVEQARA
                     KGMGVISMKLIGEGVFNREDRQKAMKFAFRNAGVDSVTVGYKNTQEIDEAIENLNMAL
                     A"
     gene            519..1091
                     /locus_tag="VN788_02215"
     CDS             519..1091
                     /locus_tag="VN788_02215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HXU19379.1"
                     /translation="MAAVQLDPKSTALVLIDLQNGIVGRTMAPHALDEVVKNAAKLAD
                     AVRSKGGTVVYVRVDVSDMLHLPVDAPSRDPNAPPPPPTASELVAGCGYQSGDLLITK
                     RQWGAFYGTGLEQQLRRRGVRTIVMGGVATNFGVESTARVGYDRGFELVFVEDAMSSM
                     SDGLHRFAVQSIFPRMGRVRTTEEVIQALG"
     gene            complement(1152..2063)
                     /locus_tag="VN788_02220"
     CDS             complement(1152..2063)
                     /locus_tag="VN788_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19380.1"
                     /translation="MDEVVFDVIMQSTGAFVGDIRNYQTEPPPEEDLELGLNCWVGHP
                     PLGISPDVIFDACSPAGYNFHPFRQFGCRYAFCRKVEPGHTDYYRWDTDGWLRRILFL
                     SRLIRPTTIGTNLSARLYFRDAELQTIVPGPTQGFGTHAWVVATSRWRDWLSVQEFER
                     LRDSIPIYNLNPPDRVRQARKHIDNAFHSFYLDQRCASIVTAFESLLKVSSFKATRQF
                     ASRVPKLAERAGLEITEPEAIALYADRSAFVHGTQVSFMDLSDELIAHYNRFEEVLRC
                     CLLRASTEPAFAALFSSDETVVDAFGM"
     gene            complement(2337..2984)
                     /locus_tag="VN788_02225"
     CDS             complement(2337..2984)
                     /locus_tag="VN788_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018974369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXU19381.1"
                     /translation="MSKLRVNCFSVSFDGYSAGPDQSLENPLGVGAMQVFNWQFATRA
                     FHQMTGQPGGETGMDNDLVNRGFENVGAWILGRNMFGPVRGPWPDDSWKGWWGDTPPY
                     HVPVFVLTHHARAPLTMEGGTTFHFVTDGIHSALHQAVKAADGKDVRLGGGVSTIQQY
                     LRAGLIEELHLVSSPVLIGRGESIFRDLDLPALGYELTSHKASPAATHMFLTRKT"
     gene            3155..3721
                     /locus_tag="VN788_02230"
     CDS             3155..3721
                     /locus_tag="VN788_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19382.1"
                     /translation="MKALDVDELTKWCNESPSGIQLEMPHHLRYATPRPFGLKIDVPS
                     EAARVAALVYCLLAVEEDVGYYGSLIWFTNWDTGTPEIERCGLRILEQMRSGYGVTQS
                     IENAPGQLFRSDEIVDAHAFLTLAMLWGWDAYFTPHGTRYFAYCRQGTSLYLVTDDEK
                     VFEKLLASLITYHPVSQLPVYLKGAQGI"
     gene            complement(4343..5068)
                     /locus_tag="VN788_02235"
     CDS             complement(4343..5068)
                     /locus_tag="VN788_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXU19383.1"
                     /translation="MKLRNPLHSLRAALPGLLVLCLTVSGCRHHHVQAVAPPPPTIRQ
                     APPASFPERAPEPTSPVLHTEIGIASWYGAPYHNARAANGEIYDENGMTAAHRTLPMG
                     TVVRVTNLSNSKSVVVTISDRGPFVPGRILDLSRAAALKIGVWRTGTARVRIDVLRYP
                     SSISSSGKWCVQIGVFHHEHDARRLQQKLQREYPSANVIDFKGATGYWVRIRPYGQSH
                     TSAEQIARVIDPVEGQAYIVRLD"
     gene            5347..6432
                     /locus_tag="VN788_02240"
     CDS             5347..6432
                     /locus_tag="VN788_02240"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU19384.1"
                     /translation="MRRLIVTWCLLMVGFASNSLFGLAARSAELPDAPVPQNGQASAK
                     PSAGYSSVTGTVEDMHGKPIAGVVVTAVKANETAGKTATVDEKGMFRISDLEPGTYIV
                     SITLPGRAPQVATQVVLGAREEQELPIVTERNPHSTTTVQVNADLTQVATAQVHLQEK
                     QRILGVVPNFYTTYLWDSAPLTPKLKFKLALRAEIDPIEFVVDAGVAGAEQYHNTYPG
                     YGGGWQGYGKRFGSAYADSAIGAMLGRALFPIWFHQDPRYFYRGTGSWSSRTLYAVAQ
                     TVVTRGDNGETQPNYSHVLGNFTTAGISNIYRAPSDRSFDLTMRDALVETAGNAVGNV
                     LREFISRPFTSNVPDFAKGRKSDKSSQ"
     gene            6698..7753
                     /locus_tag="VN788_02245"
     CDS             6698..7753
                     /locus_tag="VN788_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19385.1"
                     /translation="MAGRELAAHVVVVLLLLMPSGVLAQLPDAPTPAESVQGARQLQA
                     DWARIQNDLERDWVTRKAEWPATTTAEDSDSGAGPQQVAFAEPNGLGAQQSFAPSPKR
                     QFRPTYSSSTTYERAVPGAAPSLQSSCPVNFCPAQPLDICCQFTSSPFEQYLNWPDTI
                     PLTAKDNLRSAVRGVTDPFNLMTIAADAAIGIGSDSHTVYGPGFEGFAKYAGVSLTED
                     MTGEFFGTFLVPSIMHQDPHYHRQPFMSIKRRFLHTFAAVVWSQSYTGKPMFNYANIV
                     GGIATAVVSNTFVPGPNRQGFSNTAQRLALAFAISPSGNMIAEFVPDLASRVNLRVVI
                     FQRILNSVTLEESGVAQ"
     gene            complement(7791..8195)
                     /locus_tag="VN788_02250"
     CDS             complement(7791..8195)
                     /locus_tag="VN788_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXU19386.1"
                     /translation="MESANASIDRKIKELGDWRGKTLAKVRTLIHQADPDIVEEWKWA
                     KAASPGTPVFSRSGIVCTGETYKNAVKLTFAKGASLPDPSGLFNSSLEGNVRRAIDIH
                     EGDKINESALKSLIRAAVALNLEAKSKPKPRH"
     gene            8494..9243
                     /locus_tag="VN788_02255"
     CDS             8494..9243
                     /locus_tag="VN788_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19387.1"
                     /translation="MKIVVIGGTGLIGSKLVSKLRQRGYEAVAASPNSGVNTITGEGL
                     AEVLKGAAVVVDVSNSPDWEDAAVLKFFETSTRNLLAGEAAAKVGHHVALSVVGTDRL
                     SESGYFRAKIAQEKLIREGSIPYSIVHATQFFEFLKGLADISMVGDKVHLPPVLFQPM
                     AAEDVATGVARVAIGSPVNGIVEIGGPEQFRVDELVRRRLASLKDSREVIADPNARYA
                     GAKLSEKTLVPGNDAQLGETRFESWVAQASA"
     gene            9643..10089
                     /locus_tag="VN788_02260"
     CDS             9643..10089
                     /locus_tag="VN788_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU19388.1"
                     /translation="MNSVQIEQKSFEMPPQEGISIAYFLTVADIERSARYYEKVFGAR
                     ILSLGDGNAPAYLLLANTWMILNLGGGPTPDKPTVTLSVPDPNHINSFMNFRVADIQA
                     CYKLWKSRGADFVTEPIPKYGEIRCYIRDPDGYLIEVGQSTTLTYG"
     gene            10119..10916
                     /locus_tag="VN788_02265"
     CDS             10119..10916
                     /locus_tag="VN788_02265"
                     /inference="COORDINATES: protein motif:HMM:NF028434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019172 - glyoxalase III activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXU19389.1"
                     /translation="MPIKGKVLVLVSSGHGLPLKDGKVYAGAGYYLNELTVPVRALMK
                     EGYEITFANPKGNTPQLDVNSAIAQFFGGDEAKLQDYLKFRDSLTGLKNPTRISDVIA
                     SGLDQYDAVFVPGGHGPMIDLLDDPDAGTVMRHFHQTSKPTAVLCHGPISLLSALPNS
                     TEVVAALATGDAAGARAKAQGWIYAGYKMTIFSTAEEQQREPLEIGGKVLFYPDFALL
                     TAGGDVSVVAPWQSYVLQDRELISGQNPFSDEALLKLLLPALDEKKK"
     gene            11034..11918
                     /locus_tag="VN788_02270"
     CDS             11034..11918
                     /locus_tag="VN788_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXU19390.1"
                     /translation="MNYPTLYRTLQVDGLSIFYREAGSKDAPTLLLLHGLPSSSRMFE
                     PLFARLSDRYRLIAPDYPGFGHSDWPDPKKFAYTFDNYAEIMNHFTEALGLSRYTLYM
                     QDYGGPVGFRMILAHPQRVEALIVQDAVAHNEGLAANWKTRRAFWADRATNESALRAN
                     LLSLSATRTRHIGNDPNVERYDPDLWTDEFYFLNRPGQAEIQSDLFYDYRTNVDDYPK
                     WQAWMREKQPRLMVIWGKYELSFDPSEPERYRTDVPNAEVHIVDGGHFALDTAADQIA
                     AFVQGFIGSSRKADVRPG"
     gene            complement(12088..>12351)
                     /locus_tag="VN788_02275"
     CDS             complement(12088..>12351)
                     /locus_tag="VN788_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19391.1"
                     /translation="FGMSPVQAFEAPRFSTEHFVGSFSQDAPKFGSLSVPKSLPENVK
                     ADLRARGHVVTDGSGRVGGVALIGIDSKTNQDTAVGPAAGKVE"
BASE COUNT         2683 a         3110 c         3904 g         2654 t
ORIGIN      
        1 gcggcaggcg aagacggagt acttcgacat tatgctgctg cactttcagc actcgggaac
       61 gtggccggtg gatacggttg cgtggcagga cggcattgaa gaggcggagc ataagaaggc
      121 ggtgctttct cgcggagcca gtgtgcatgg gttgccggcg ctgcggcagg tgccgggaga
      181 ctcgtggctg gatgtggcca tgatccggat gaatcacaaa ggcgtgcaca tggatgcgga
      241 ggattacgcg accgacggcc tgggcaatgt gccggaggtg gtgaagcatg tggagcaggc
      301 gcgcgcgaag ggtatgggcg tgatcagcat gaagctgatt ggcgagggcg tatttaatcg
      361 cgaagatcgg cagaaggcga tgaagttcgc gtttcgcaat gcgggcgtgg acagcgtgac
      421 ggtgggttac aagaacacgc aggagattga cgaagcgatt gagaatttaa atatggcgct
      481 ggcgtagccg atcgaaacca atagaggagc ggggcggaat ggcggcagtg cagctggatc
      541 cgaagtcgac ggcgctggtg ttgattgatt tacagaatgg aattgtgggg cggacgatgg
      601 ctccgcatgc gctggacgaa gtggtaaaga acgcggcgaa actggcggat gcggtgcggt
      661 cgaagggcgg cacggtggtg tatgttcggg tggatgtttc ggacatgctg catttgccgg
      721 tggatgctcc gtcgcgcgat ccgaatgcgc cgccgccacc gcctacggcg tctgaactgg
      781 tggcgggttg cggataccag tcgggcgacc tgctgattac gaagcggcag tggggcgcgt
      841 tttatgggac cgggctggag cagcaattgc ggcgtcgcgg agtgcgcacg attgtgatgg
      901 gcggggtggc aacgaacttt ggcgtggagt cgacggcgcg ggtggggtat gaccgcgggt
      961 ttgagctggt gtttgtcgaa gatgcgatgt cgagcatgag cgacgggctg catcgcttcg
     1021 cggtgcaatc gatctttccg aggatggggc gggtaaggac tacggaggaa gtgattcagg
     1081 cgctcgggta ggatttttag cgagcgatac tgctcgagta gacggaaacc ttggacaggg
     1141 aacctgctgc attacatccc gaaagcatcc actacggttt catccgaact gaaaagagcg
     1201 gcaaacgcgg gttcggtact ggcgcgaaga agacaacagc gaagtacttc ctcgaaccgg
     1261 ttgtaatggg cgatcagttc atcgctaagg tccatgaagc tgacttgggt tccatggaca
     1321 aatgctgacc ggtcggcgta gagcgcaatg gcttccggtt ccgtgatttc gagcccagca
     1381 cgttccgcca attttggcac tcgtgacgcg aactgcctgg ttgccttgaa ggaagagact
     1441 ttcagcaaac tttcgaaggc tgtcactatc gaagcgcagc gttgatcgag gtagaagcta
     1501 tggaacgcat tgtcaatgtg cttgcgggct tggcgcacgc ggtccggcgg atttaagtta
     1561 tatatgggaa ttgaatcgcg cagccgctcg aactcttgta cagacaacca atcacgccaa
     1621 cgtgaggtcg caacgaccca ggcatgtgtt ccgaagccct gcgttggacc tggaacgata
     1681 gtttgaagtt ctgcgtctct aaaatagagc cttgcagaga ggttcgtgcc gatggttgtc
     1741 ggccgaatca gtcgggatag aaaaagaatg cgtcgcagcc agccgtcagt gtcccaccga
     1801 tagtagtcgg tatgtccggg ttctactttc cgacagaagg cgtatctgca gccaaattgc
     1861 cggaatggat ggaagttata ccccgccggg ctacaagcgt cgaagatcac atcaggactg
     1921 ataccaagcg gcggatgccc tacccagcag ttgagcccaa gttcgaggtc ttcttccggc
     1981 ggaggttctg tttgatagtt gcgtatgtcc ccgacaaatg cgcctgtcga ttgcatgatg
     2041 acatcaaaaa ctacttcgtc cataagatta ggctacttct gattcgcact cagacgtcgg
     2101 acgaggaaat taaatattgc tgctagctgc gatgcccggc gaatcattag tttcagccgt
     2161 acgccatgtc tacctcgtac atgggtcagt aagcgttgaa tcgggaaaag aggaggtcgg
     2221 tctctcacgg agacacaatt agctcggcac tcgatcggca aattaagatt tggccaacgc
     2281 ttggtttgct gtgcgagagg cggacgattt atttgcgaag agaattcgca tgggggttat
     2341 gttttgcggg ttaggaacat gtgggtggcg gcgggggagg ctttgtggct ggtgagttcg
     2401 tagcctagcg cggggagatc gaggtcgcga aagatgcttt cgccgcgacc gatcaggacc
     2461 ggcgaggaga cgagatgcag ttcttcgatg aggccggcgc gcaggtattg ctggatggtg
     2521 gagacgcctc cgccgaggcg aacatctttc ccgtcggctg ctttgacggc ttggtggagg
     2581 gcgctgtgga tgccgtcggt gacgaagtgg aaggtggtgc ctccttccat ggtgaggggc
     2641 gcgcgcgcat gatgggtgag aacgaagacg ggcacgtgat agggcggggt gtcgccccac
     2701 caacctttcc aggaatcgtc tggccaggga ccgcgaacgg ggccgaacat gttgcggccg
     2761 agaatccagg cgccgacatt ttcaaagccg cgattgacga ggtcgttgtc catgccggtt
     2821 tctccgccgg gctggccagt catttgatga aaggcgcgcg tcgcgaattg ccagttgaag
     2881 acttgcatgg cgccgacgcc gagcggattt tcgagggatt gatcgggacc ggcgctgtaa
     2941 ccgtcgaagg aaactgaaaa gcagttgacg cgaagcttgg acatcgaggg ttcctctcaa
     3001 ggtgcatctg cgaacagttt acgatgggag gattcggcga acgtgaggat tgaggtgttg
     3061 tacatcccac ccttcgcaaa gacagcgaag aacggggccc cctcaagttt gtaaataacg
     3121 aaagcatggg ctaccagcca tttaggagaa cacgatgaaa gcccttgatg ttgatgagtt
     3181 aactaagtgg tgtaacgagt caccctcagg tatccagctt gaaatgcccc atcatttgcg
     3241 ttatgcaaca ccgcgcccat ttggtctcaa aatcgacgtg ccgtcggagg cagccagggt
     3301 ggcggcgctc gtctattgcc tattggcagt cgaggaggat gttggatatt acgggagcct
     3361 catctggttc acgaattggg acacaggcac gccggaaata gagcggtgtg gattgcgaat
     3421 tctggaacaa atgcgaagtg gatacggagt cacccaatcg atagagaacg ccccgggcca
     3481 actgttcagg agtgacgaaa tcgtggatgc ccatgctttc ttaacactcg caatgctttg
     3541 gggctgggat gcgtacttta caccgcacgg gactcgatat tttgcttatt gccgacaggg
     3601 tacttcactt tacctggtta ctgatgacga aaaggtattc gagaagctgc tggcctcgct
     3661 tattacatat catccggtgt cgcaacttcc tgtttaccta aaaggcgcac aagggatata
     3721 gagggttgcg aagagcaatt cgcgtatgaa gacgagatat gggaaaattg cgcgaaggtg
     3781 catggataca ggcgatttat gcacaattct tccgcgattt gcaccgaaat aaattcgtag
     3841 aattgcggga atgtcgtggc atactcattt caacggaaga gcaatctttc agagaagaaa
     3901 gtggaacgaa agccggccgg gggctcggca acggacccca attcgggcaa ccgaaggcgg
     3961 caggcggacg gaaagctaac acgacatgaa agaccggtag gtcaagggag gtgcgggctt
     4021 cgctcgcatg gcctaagtca gccgggacga cagagttgtg atctggtcac cgaaacacca
     4081 gatcttcgaa agttcggatc gctgggggcg agttctcgca agaggattcg ccctttggca
     4141 tttttatgca ccgagattat gcggagggcg ggccagaatc ccatgtctca aaagcgagac
     4201 atggggcacc caggatttct cccctcccac tcaagccaac gattatgcgg agggcgggcc
     4261 ggaatcccac atctcaaaag cgagatgtgg ggcacccagg cgacctcaga atgacgctct
     4321 tcattccatg gcggataaaa agtcagtcga ggcggactat gtaggcctgg ccctcgacgg
     4381 gatcgatcac gcgggcgatt tgttcggcgg aggtgtgcga ctggccgtag gggcggatgc
     4441 ggacccagta gccggtggcg cctttgaaat cgatgacgtt ggcgctggga tattcgcgct
     4501 gcagtttctg ctgaaggcgg cgggcgtcgt gttcatgatg gaagacgccg atctgcacgc
     4561 accattttcc tgatgaagaa attgaggacg gatagcggag cacatcgatg cggacgcgcg
     4621 cggtaccggt acgccagacg cctattttta gggcggcggc acgggacaag tccagaatgc
     4681 ggccgggaac gaagggtccg cgatctgaaa tggtgacgac gacggactta ctgttcgaca
     4741 aattggtgac gcggacgacg gtgcccatgg gaagggtgcg atgggcggcg gtcatgccgt
     4801 tttcgtcata gatttcgcca ttggcggcac gcgcgttgtg atagggtgcg ccgtaccaac
     4861 tggcaattcc aatttcagta tgaaggaccg ggctggttgg ttccggagcg cgttcgggaa
     4921 aggacgcggg cggcgcttgc cgaatcgtcg gcggcggcgg tgcgacggcc tggacgtgat
     4981 gatgacggca gccggagacg gtgaggcaca ggaccagcaa tccgggcaaa gcagcgcgaa
     5041 gggagtgcag gggattccga agcttcacag tttctaatgc tatcgtgttg cagccgggag
     5101 gagagcgctg tggccgcgaa tgagcgttgt caattgagat gccacgcgag gaaccgcttg
     5161 ttttcgcaac agattccgcg atctgcaacc ggcggaaggc aagagcgtgc atcttaagag
     5221 aagaaaatga atctatcgag gtatgatcaa agaaatctac ctcaagaggg atgcaatcct
     5281 ctgatcacgc gccgtatccg ggaacgccca agacttagac tttcaaaact ttgccaggag
     5341 tacactttgc ggcgcctgat tgtgacatgg tgtttgttga tggtggggtt tgcgtcgaat
     5401 tcgctctttg gcctggcagc acgatccgcc gagttgccgg atgctcctgt gccgcagaat
     5461 gggcaggcga gtgcgaagcc gagcgccggg tattccagcg tgacgggtac ggtagaggac
     5521 atgcacggga agccgattgc gggcgtggtg gtcacggcgg tgaaagccaa cgagacggcc
     5581 ggcaagacgg ctacggtgga tgagaaggga atgtttagga tttccgatct tgagccggga
     5641 acgtatatcg tttcgattac gctgccgggg cgggcaccgc aagtggcaac ccaggtagtg
     5701 ctgggagcgc gggaagagca agagctgccg atcgtgacgg agagaaatcc gcactccacg
     5761 acgaccgtgc aggtgaatgc cgacctgacc caggtggcga cggcgcaggt gcatttgcag
     5821 gagaagcagc gaatcctggg agtggtgccg aatttttata caacctattt gtgggattcc
     5881 gcgccgctga cgccgaaact gaaattcaaa ctggcgctgc gagcggagat cgatccgatt
     5941 gagtttgtcg tggacgcggg cgtggccgga gcggaacagt atcacaatac ctatccggga
     6001 tatggcggcg gctggcaagg gtacgggaag cgattcggat cggcgtatgc ggactctgcg
     6061 attggcgcga tgctgggacg ggcgctgttt ccgatctggt tccaccagga cccgcgctat
     6121 ttttatcgcg gcaccggcag ttggagctcg cggacgttgt acgccgtggc gcagacggtg
     6181 gtgacccgcg gggacaatgg cgagacgcag ccgaactact cgcatgtgct agggaacttt
     6241 acgacggctg ggatttcgaa catctatcgt gcacccagcg accgcagctt cgacctgacg
     6301 atgcgggatg cgctggtgga gacggcaggc aacgcggtcg gaaacgtgtt gcgagaattt
     6361 atttcacgtc cgttcacgtc caatgtgccg gattttgcca agggcaggaa aagcgacaaa
     6421 tcctcacagt aatctggccg atcgttcgcg tgaggcaact ttttctcttc tgaaatggtc
     6481 tatcgtaaag acatacagca ctcggacgct tgggataggt ctgattcact cagggagaaa
     6541 tccaggtatc gacggcatcc gccgcggcgg atagctctgc cctgatatcg atcagactgc
     6601 atcggagttt tgatgtgttt ggtcgtggag aggggcggtc cggcggctct ggcctgcgtt
     6661 gattgcgctg caggtcgatt ccattgcgga caaaggaatg gcagggagag aactggcggc
     6721 tcatgttgta gttgtgctgt tgttgctgat gccgtcggga gtgctggcgc agttgccgga
     6781 tgcgccgacg ccggcagaaa gtgtgcaagg ggcgcgacaa ctccaggccg actgggctcg
     6841 aatccagaac gatttggaac gcgattgggt aacgcggaag gcagagtggc cggcaacgac
     6901 cacggcggaa gattccgatt ctggcgcggg accgcagcag gttgcgttcg cggagcctaa
     6961 cggattgggc gcgcaacaat cttttgctcc gtcgccaaaa cgtcaattcc ggcccaccta
     7021 ttcctccagc acgacttacg agcgggcggt tcccggtgcg gcaccttcgc tgcagagtag
     7081 ctgccctgtg aatttctgtc cggcacagcc actggacata tgttgccagt tcacgtcatc
     7141 gccattcgag cagtatttaa attggccgga tactattccg ctgacggcga aggataattt
     7201 acgctcggcg gtgcgcgggg tgacggatcc attcaacctg atgacgattg cggccgacgc
     7261 ggcgatcggc attgggtctg actctcatac cgtgtacggg ccgggcttcg aaggttttgc
     7321 gaaatacgcg ggcgtgagct tgacggaaga catgacggga gaattttttg ggacgtttct
     7381 ggtgccgtcg ataatgcatc aggatccgca ttatcatcgt cagccgttta tgtcgatcaa
     7441 acggcgattt ctgcatacgt ttgcggctgt agtgtggtcg cagagttaca ccggtaagcc
     7501 gatgttcaac tacgccaata tcgtcggcgg cattgcgacg gcggtggtga gcaatacatt
     7561 tgtgccgggg ccgaaccggc agggattcag caatacggcg cagcgactgg cgctggcgtt
     7621 tgcgatcagt ccgtcgggca acatgatcgc ggaatttgtg cccgacctgg cgagccgcgt
     7681 gaacctgcgc gtggtcatct tccagcggat attgaattct gtgacgctgg aggaaagcgg
     7741 cgtggctcaa tagtgggatg tgggatggta tggaggctta gccagcgggt ttagtggcgc
     7801 ggctttggct tgctcttggc ttcgagattg agggctacgg cggcgcggat gagggatttc
     7861 aaggcggact cgttgatctt gtcgccttca tggatgtcga tggcgcgacg aacgttccct
     7921 tcgaggctgg agttgaacag gccggatggg tcgggcaagg atgctccttt ggcgaaggtc
     7981 agcttgacgg cgttcttgta agtttcgcca gtgcagacaa tgccgctgcg ggaaaagacg
     8041 ggcgttcccg gcgaagctgc cttggcccac ttccactctt cgacgatgtc cgggtcggcc
     8101 tggtgaatga gagtgcgtac tttcgcgagc gtttttccgc gccagtcgcc gagttctttg
     8161 atcttccggt caatgcttgc attggcggat tccataggta tgggtcctct cctgtacgga
     8221 ttcaactctc gcccttaatt tagatacgtt cagcggtatc tgcaactgtg ttccgcatcc
     8281 aataaaagag ggaagcggcc aaggagctat gacgggccct gaagaacccg gtttggctga
     8341 accggcctca cggaagtatc cttctcaacc tccacgattt gctcaaccac aacgagctct
     8401 tgacgggata atgttcagaa cgatgggatg ctcgctgtta acgaatttac cggcatgttc
     8461 gcaatgccga ggtcgaaaac cagggagaca atcatgaaaa tcgtagtgat cggcggcact
     8521 gggctcattg gatcaaaact tgtcagcaag cttcgtcagc gcggatacga ggcggtagcg
     8581 gcatcaccta attcgggagt caacactatc acaggtgagg gactggccga agtgctgaaa
     8641 ggtgcggcgg tggtcgttga cgtatccaac tctcccgact gggaggatgc agcggtgctg
     8701 aagttcttcg aaacatccac ccgcaatctg ctcgccggtg aagccgccgc aaaggtagga
     8761 catcacgttg cgttgtcggt ggtaggaacg gatcgcttgt cggaaagcgg ttatttccgc
     8821 gccaagatcg ctcaggagaa attgatcaga gaaggttcaa tcccgtactc gatcgtgcat
     8881 gccacgcaat tttttgagtt cctcaagggc cttgccgaca tttccatggt cggagacaag
     8941 gtgcatctgc ctcctgtgct ttttcagccg atggctgccg aggatgtcgc aacgggcgtg
     9001 gccagggtcg ccataggttc gccggtaaac ggcatcgtcg aaattggagg gccggaacag
     9061 ttccgcgtgg atgaactcgt tcggcggcgc ttggcatcgc tcaaagattc acgcgaagtc
     9121 atcgctgacc caaacgcgcg ttacgcagga gctaaactca gcgagaaaac actggttcct
     9181 ggcaatgacg cgcaactcgg cgagacgcgc ttcgagagct gggtggctca ggccagcgcg
     9241 taaattccga atgcgtatcc tcggcccaca gtcggtgcag tgggtgcgag tttgccgtga
     9301 aggtctgcga gttcccaatc ctttcgtcaa ggccgattac tcccgcgaag tcggcttctc
     9361 atgagcaatc cattgcttct gacaaaggcg tttgaacttc gcgcggggca gtcaagacag
     9421 agtcgtaagg tttctcgtta ccgatccaac ttcatactgc cgcgaacgac taagcgtccg
     9481 aggagggcgc tacctgtctc ctggagcgga caatcaaatt caaattcggc ctggccgagg
     9541 tgcgcgactt cacgtcgtcg caaggcgacg gaattggagt atcaaacgac gtgtttaagg
     9601 tgtttaattg ccgctggttc tgtatcggaa aaggagttgc taatgaacag cgtacaaatc
     9661 gagcagaaga gctttgagat gcccccgcag gaggggatca gtatcgcgta ttttctcacc
     9721 gtcgccgaca tcgagcgatc ggctcgctac tacgaaaagg tgttcggcgc tcgcattctg
     9781 agcctgggtg acggcaacgc gcctgcgtac cttttgctcg cgaatacctg gatgattttg
     9841 aacctcggag gcggcccgac accggacaag ccgacggtaa cgctcagcgt tccggacccg
     9901 aatcacatca acagctttat gaatttccgc gttgcggata ttcaagcttg ctacaaatta
     9961 tggaaaagtc gaggagccga tttcgtcacg gagccgattc ccaagtatgg cgagatacgc
    10021 tgctacatcc gcgatcctga cggttatctt atcgaggtcg gacagagcac tacgctcacg
    10081 tatggttaga ccgtcattcg atttcagagg agaacgaaat gccaataaag ggaaaagtcc
    10141 tggtgctagt ttcgagcggt cacggcttgc cattgaaaga tggcaaggtt tatgccggcg
    10201 ccggctatta tctgaacgaa ttgacggttc cggttcgcgc cctgatgaag gaaggctacg
    10261 aaatcacttt cgccaacccc aaaggcaaca cgccgcaatt ggatgtcaat tcggctatag
    10321 cccagttctt cggtggagac gaggctaagc ttcaggacta tctgaaattc cgcgacagcc
    10381 tgaccgggct caaaaatcca actcgtatct cggatgtcat cgcatcgggc ctggatcaat
    10441 atgacgctgt tttcgtcccc ggcggccatg gcccgatgat cgatcttctc gacgatcccg
    10501 acgccggcac cgtgatgaga cactttcatc aaacatccaa gcctacggcg gtgctctgcc
    10561 atggcccgat ttcacttctc tcagctcttc cgaattcgac tgaggtggtc gcagcgctgg
    10621 cgacgggcga tgccgctgga gcgcgggcaa aggctcaagg atggatttat gcgggataca
    10681 agatgaccat cttttcgacg gcggaagagc agcagcgaga gccgcttgag attggcggta
    10741 aggttctctt ctacccggac tttgcattgc ttacggcggg cggcgacgta agtgtggtcg
    10801 ctccatggca gagctacgtc cttcaggatc gcgagttgat ctcaggtcag aacccgttct
    10861 ctgatgaagc gcttctgaaa cttcttcttc cggcactcga cgagaagaaa aagtaagttg
    10921 gcaacagggg ggtgtcccat cctccgttgg acagatctcg acttttcatc gcaatcggcg
    10981 cctggcttcg ccctggccgc ccggtttttt tgacgcaatg aaaggaatgt tcgatgaact
    11041 accccactct ctatcgcacc ctgcaggtcg acggcctgtc gattttctat cgagaggctg
    11101 gatctaagga tgccccaacg cttctcctgc tgcatggact tccttcttcg tcgcggatgt
    11161 tcgagccgct cttcgcaagg ctctcggaca ggtatcgcct gatcgcgcct gattatcccg
    11221 gtttcgggca tagcgactgg ccggacccga agaaattcgc atacaccttc gacaattacg
    11281 ccgagatcat gaaccatttc accgaggcac tcggcctctc gcgctacacg ctctacatgc
    11341 aggattatgg cggcccggtg ggctttcgca tgatcctggc gcacccgcaa cgcgtggaag
    11401 ctctcatcgt tcaagacgca gttgcgcaca acgaaggcct ggccgcgaac tggaagacgc
    11461 ggcgagcctt ttgggccgat cgcgcgacca atgaaagcgc gctgcgcgcg aacttgctct
    11521 cgctgtccgc aacacgcact cgtcacatcg gcaacgatcc gaacgtggaa cgctacgacc
    11581 ccgatctctg gacggatgaa ttctattttc tcaatcggcc cggccaggct gagattcaga
    11641 gcgatttgtt ctatgactat cgaaccaatg tggatgacta cccgaagtgg caagcgtgga
    11701 tgcgcgagaa gcaaccgcgg ctcatggtga tatggggtaa atatgagctt tcgttcgatc
    11761 cgagcgagcc ggaacgctat cgcacagacg tgccgaatgc tgaggttcac atcgtcgacg
    11821 gaggacattt cgcattagac acggcggcag atcagatcgc tgcgttcgtg cagggattta
    11881 tcggttcttc acgcaaggcg gacgtacggc cggggtgagg cgcgcttctg ccaatgttgc
    11941 ctcaaacaat cgccaactcg ggctatggct gtgccgcact actatcttcg gcttgatggg
    12001 tcgggcccgc gtcaggatca aggtctcgca aacgccgcta cgaaattttt agaaaactcc
    12061 attcgttgtg cacccactat tactggtcta ttccactttt ccggcggccg gacctacggc
    12121 ggtgtcctga ttcgtcttcg agtcgatgcc gatcagcgct accccgccga cccggccact
    12181 gccgtcggtc actacgtggc cacgcgccct caggtccgcc ttgacgtttt ccggcaggga
    12241 tttcggcaca gagaggctgc cgaatttggg agcgtcctgg ctgaaggagc cgacgaaatg
    12301 ttcggtggag aagcgcggcg cttcaaacgc ctgcacggga ctcatgccga a
//
LOCUS       DATNDT010000076         2778 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3802677, whole genome shotgun sequence.
ACCESSION   DATNDT010000076 DATNDT010000000
VERSION     DATNDT010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3802677"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..996)
                     /locus_tag="VN788_02280"
     CDS             complement(169..996)
                     /locus_tag="VN788_02280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU19392.1"
                     /translation="MKAYSPKEYWAGLADNFHSADTAGFAPILHPGAPAWFNSRIDHL
                     QFCAMRRALALAEVSRPARILDVGCGTGRWLRRYCDLGFDATGIDATPQMLRLARGRG
                     TMAPLVTGEANRLPFRSAEFDVVSDVTVIQHIPAARQEQALLEMTRVLKPGGRMILFE
                     LIRGEDLHIFPRNPQSWMDLGASCGLRARGWFGQEYLLLDRLFVRVARGVMKRRETSV
                     ATGVDSAETMSSRATILRRVYWELRHGIVPLSAWAEPIVGRLCPGRLATHGVFVFQK"
     gene            complement(1033..2232)
                     /locus_tag="VN788_02285"
     CDS             complement(1033..2232)
                     /locus_tag="VN788_02285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU19393.1"
                     /translation="MSATESRPRLAIVSPFLDRSHGTERIVVEWISVLSETFEIHVYS
                     QDLEDVDRGKVTWHRISKLPGPHLFNFIWWFAANHCRRVWDRCVRDLRYDVVFTPGAN
                     CLDADVISVHIVFGQFLRSGQHELALGGNPPRTWPRLVHRRIYYRLAIALERRAYRNS
                     RTLLVLLSRKTASDLAKLYGRRNRMHVLYPGLDHSSFNPDRRAALRDGARTQLELPGD
                     RIAVLMVGNDWRQKGIRVLLDAMTKLRDLPVNLMLVGEEDPQPFLLMVNERNLNGRVR
                     FLLPRKDIEFYYAAADVYAGPSLEDTGPLPPVEAMACGVPAILSASCGTAEIITDGVN
                     GLILEDPTDATALAAMVRRLCDDEEFRERLGQNAAAKAREFTWESNGQELARIFEEIV
                     RQKARVT"
     gene            complement(2288..>2778)
                     /locus_tag="VN788_02290"
     CDS             complement(2288..>2778)
                     /locus_tag="VN788_02290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19394.1"
                     /translation="DPESSAYAGHNRAWDYPIANLEIAFQRPNWVMGNGIGTASLGMQ
                     YVDQVVGQARPQLWVEEGYGVLIVEMGIIAPFLWILWTAALLYYSWKIVRRLKGTRFA
                     PLGIAILWYAFFLLYPLTYVGLSPYQNYICNAYLWLLVGMLFRLPDLLENATAPNPVL
                     LP"
BASE COUNT          651 a          852 c          747 g          528 t
ORIGIN      
        1 ccggtgaatc attgaggctc aaaaaatccg cacgccaacc gtgcgcgtga gcaatttcaa
       61 cgagagccac ggcagtcagc cttccggccg cctgaatccc gccaattcca agcagttcag
      121 gaaacagccc gatgattcgc ctcgtcgacg gactgaattt tcccatgctc acttttgaaa
      181 aacgaaaacg ccgtgagtcg ccaaacggcc agggcagagt ctgccgacga ttggctccgc
      241 ccatgccgaa agtggaacga tgccatgccg aagctcccag tagacgcgac gaagtatcgt
      301 cgcgcgtgac gacatggttt ccgcggaatc cacaccggta gcgacagaag tctcgcgtct
      361 cttcatcact cctcgcgcaa ctcgaacgaa gagcctatcc agcaaaagat attcctggcc
      421 aaaccagcct ctcgcgcgaa gtccgcacga tgcgccaagg tccatccagc tctgtggatt
      481 cctcgggaat atatgcaagt cttcccctcg aataagctcg aataaaatca tgcggccgcc
      541 cggcttgagc acacgagtca tttcgaggag agcctgctct tgccgcgccg ccgggatgtg
      601 ctggatcacc gtcacgtccg ataccacgtc gaattccgcg ctccggaaag gcaaacgatt
      661 ggcctcgccg gtgacaaggg gcgccatcgt cccgcggccg cgcgccagtc gaagcatttg
      721 aggagtggca tcgatgcctg tggcatcaaa ccccaggtca cagtatcgac gaagccatcg
      781 gcccgtgccg caacccacgt ccaaaatgcg ggcgggacgc gaaacctctg cgagtgccaa
      841 agcccgccgc atcgcgcaaa actgcaaatg gtcgatgcga gagttgaacc acgcaggggc
      901 tcccggatgc aggatgggcg caaatcccgc agtgtcggcg gaatgaaaat tgtcggccag
      961 gccggcccaa tattcctttg gtgaatacgc tttcataggt ctctaaaaac ggactacttc
     1021 acgccgtggt catcacgtaa cgcgggcttt ctgacgcacg atctcttcaa atatccgcgc
     1081 cagttcttga ccattgcttt cccacgtaaa ctcgcgggct ttggcggcgg cgttttgacc
     1141 aagccgctcg cgaaactcct catcgtcgca gagtcgccgg accattgcag cgagtgcggt
     1201 cgcatcggtt ggatcttcaa ggatcagccc gttcactcca tcggtgataa tttccgccgt
     1261 cccgcaggac gcggaaagaa tcgcgggcac gccgcaagcc attgcttcca cgggcggcaa
     1321 cgggcccgta tcttcgagcg agggcccagc gtacacatcc gcagcggcgt aataaaattc
     1381 aatgtctttt cgcggaagga ggaaacgcac ccgtccattc aagtttcgct cgttcaccat
     1441 aagcaggaac ggctgcggat cttcctcacc cacaagcatc aggttcaccg gcagatcgcg
     1501 cagttttgtc atcgcatcca ggagaacgcg aatgcccttc tggcgccagt cgttgccaac
     1561 cattaggacc gcgatccggt cgcccggcag ctccaactgt gttctcgcgc cgtcgcgcaa
     1621 cgccgcacgc cgatctggat tgaaagagct atggtcgagt cccgggtaaa ggacgtgcat
     1681 gcgattgcgg cgtccgtata gcttcgcaag atcgctcgcc gtctttcgcg agagcagaac
     1741 caaaagagtc ctactgtttc ggtaggcacg ccgttccagc gctattgcca gtcgatagta
     1801 tatgcgccga tgcacgagcc gcggccaggt ccgcggcgga ttaccgccca gcgccaactc
     1861 atgctgcccg ctacggagga actgaccaaa cacaatgtgc accgatatca cgtccgcgtc
     1921 gaggcagttc gctccgggcg tgaaaacaac atcgtaccga aggtcgcgaa cgcaacggtc
     1981 ccagacccgt cggcagtggt tggcggcgaa ccaccaaatg aaattgaaga ggtgcggccc
     2041 cggcagcttc gaaatgcggt gccatgtaac tttgccacga tccacatctt ccagatcctg
     2101 gctgtaaacg tgaatctcaa aagtttcgct caaaaccgaa atccattcga ccacgatgcg
     2161 ctcggtccca tggctcctgt cgagaaacgg ggaaacaatc gcgaggcgag gcctggattc
     2221 cgtggcgctc atgctctctt gctcggcgcc atcagcagtg ggccgaaaca gtatttacag
     2281 gcgctgctca cgggaggaga actggattag gggccgtggc gttttctaga agatccggca
     2341 gccgaaaaag catccccacc aaaagccaca agtacgcatt gcaaatgtaa ttctgatatg
     2401 gcgagaggcc aacatacgta agcggatata gcaaaaagaa ggcgtaccac aaaatggcaa
     2461 tgcccagggg ggcaaagcgc gttcctttta gtcgccgtac aattttccag gagtaataga
     2521 gtagcgccgc cgtccataga atccacagaa acggagcaat gatccccatt tcaacgatca
     2581 gtactccata tccctcctca acccacaact ggggtcttgc ttgccccact acctgatcga
     2641 catattgcat cccgagagag gcggtcccga tgccgttacc catcacccag ttcggtcgct
     2701 gaaacgcaat ttccagatta gcgatagggt agtcccatgc gcggttgtgc cccgcgtatg
     2761 cggagctctc gggatcca
//
LOCUS       DATNDT010000077         7725 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_54298, whole genome shotgun sequence.
ACCESSION   DATNDT010000077 DATNDT010000000
VERSION     DATNDT010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7725
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54298"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..825
                     /locus_tag="VN788_02295"
     CDS             <1..825
                     /locus_tag="VN788_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXU19395.1"
                     /translation="NGANLASTASALPQGPEVPLSRTNSSAVASTHNAISSDSATDHN
                     PQALAAEARSVQAAPTHAMSTPPRVTNDSPASARTVASIPSRSLQSERQAAPGGQPTY
                     TQSFDPSPFDGAAQALTSSAQPAHPEGGGIVADRFENAPHAASAPQTAETTVPSEVAA
                     DAPETRVLPGGPPGASVGATVGSPGHIESSQLIHSVQPVYPLTAKQLHVEGDVELRVV
                     VGVDGTVRSVGLVSGPPSLVMAAIDAARQFLYKPALLNGKAIETVQTIEMSFKLKD"
     gene            891..980
                     /locus_tag="VN788_02300"
     tRNA            891..980
                     /locus_tag="VN788_02300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:925..927,aa:Ser,seq:tga)
     gene            complement(1036..1269)
                     /locus_tag="VN788_02305"
     CDS             complement(1036..1269)
                     /locus_tag="VN788_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19396.1"
                     /translation="MRSPSSFMETFRRHGLQLLTGLAGQEPPTPWKKRSIAVGIKEVD
                     DRIWLVTFMDDLGYIDLEEKTLQPLNNPFGPKA"
     gene            1364..2170
                     /locus_tag="VN788_02310"
     CDS             1364..2170
                     /locus_tag="VN788_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU19397.1"
                     /translation="MDTPAISTTNLTRRFGDFVAVDSINLRVTPGQFFGFLGPNGAGK
                     STTIKMLTGLLAPSAGRMEILGLDLASHSLEVKQHIGVVPEGMALFGRLTGPEYLNFA
                     GRMYGLNRETAAKRTTELLEFMQLDREPKKLITDYSHGMQKKLALAAAVIHGPKILFL
                     DEPFEGVDAIASGTLKAMLQRMILRGATIFLTSHVLEIVERLCSHLAIIHQGKLVAQG
                     SLEELRSGVQAMPADQHLAGAQPEKLTLEEIFLRIVGGARSEDQELSWLT"
     gene            2158..3831
                     /locus_tag="VN788_02315"
     CDS             2158..3831
                     /locus_tag="VN788_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19398.1"
                     /translation="MADVAPTGVPLALPDAGMQLRTIALLRWQIFKNSLRTMRGRLEA
                     VSWIFVGFWFVALGLGGAFGIAAASWWMVSHNRSDLLAALFWPIFIFWICFPLVATAF
                     TEVFDSSNLLRYPLRYSSFFLVNLIYGSLDGGTVVGLLWLIGAVLGISIAQPSSTPWA
                     VLALAIFGAANIFLVRALFSWIERWLAQRKTREIMGIIFFLIIIGFQFIGPLTQRAHR
                     QHFQLPAYISQAILIQKFLPPGLVAGALGGSVRADWLSAAGSLLLLSAYAGVFCLLFH
                     IRLRGLYAGESFSESVSREKVSMEKAETQSGWALPGLSGQLAAIMEKEARYLSRSGPM
                     LFALIMPIVVLLLFHMGGNPNRPNAVAHNPNLAFPIGAAYCLLLLTNLIYNNFGADGM
                     GVQFFFVAPVKMRSVIIAKNLIHGGVLALEMCLVFVATYFMYGSPAPAVVSATFVGVL
                     FAIPLDFAVGNLLSLYSPKKYDYGAFGRQRAPGLTVLASFGVQAVTIGIAIVVVLVAR
                     HYGSLWLATIVFAALALAAWALYQAVLGRIDGIALSKRESLIATIAKTG"
     gene            3883..4533
                     /locus_tag="VN788_02320"
     CDS             3883..4533
                     /locus_tag="VN788_02320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HXU19399.1"
                     /translation="MPGPAFRAPRYIAIEGPIRVGKSTLARILAEQLHARRIFDSEDN
                     PFLAQFYDEKPGAAFRAQMHFLYERYRRLADAAADEKAGPLLSDFIFEKDKIFAYINL
                     DNEELRLYEKYFDLLSPSLPVPDLVIYLQAKAEVLRKRVSKKGDPSETEISAEYLEAV
                     ANAYEHFFFRYSASNLLVVDTSEIDFVEHNQDLQELLNRLRQPVKGTQYFLPLGQG"
     gene            complement(4620..5105)
                     /locus_tag="VN788_02325"
     CDS             complement(4620..5105)
                     /locus_tag="VN788_02325"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU19400.1"
                     /translation="MSHWIGAASPVDAGMTDFKECLKLLRDGEAGEALLHARRALGTA
                     PKNPFYLSYTGLLAAIAERRFVDGESLCREALGLRHNHAQLYLNLAEVYVQAGRTPDA
                     IQTLEKGMISAGRDFRIRRALEKIGTRREPVFSFLDRGHPLNRRLGKLRHRMSGPSKA
                     A"
     gene            complement(5290..6060)
                     /locus_tag="VN788_02330"
     CDS             complement(5290..6060)
                     /locus_tag="VN788_02330"
                     /inference="COORDINATES: protein motif:HMM:NF016623.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HXU19401.1"
                     /translation="MVALLLAGCSKKPARKARAKKEESETRSAHREPAFRSARRLAII
                     IDDMGNDRAEDDAVIALPYPVTVSVLPHLPLSTTIADEAARRGDEVMLHVPMQPDSES
                     TAHEAVELRPGMSAKEVRSVFAGMLETVPHAAGVNNHEGSKATADPQLMDALMPALRE
                     RGLFFVDSRTTASTVAYNTARRYGVRAASRKVFLDDALNANAIEKQIDLAIKDADRDG
                     QAIAIGHPHPETIATLRQMLPRLKEDGIQLVFASDLAH"
     gene            complement(6170..7378)
                     /locus_tag="VN788_02335"
     CDS             complement(6170..7378)
                     /locus_tag="VN788_02335"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXU19402.1"
                     /translation="MSNLSKAIVVVLTVVAFAYVGVGYVLAHSSDDKAYQALTVYSEV
                     MDRIQRDYVDEPNIPKVTSGALHGLLDSLDAESSYMSPLEYKDYKQRILVKDKATAGM
                     ALTKIRAGYISVLSVLPGSPADKLGLQYADVLEKIGDFNTEQMSIEQARLLLSGDPGA
                     TVKLSVITRAKPEPQEMNITLAKIAPPKVTEAKLQDNVGYIGVPEFDESTTKEIHDDL
                     LQMSHEGEQKLILDLRNCSLGEDAEGIATAQLFVPSGTITTLKGQTVSPVVSSADPSK
                     VVWNGPVTVLIGNATAGPAEIVAAAISDNKRGQTIGDRTFGKASMQKLIPMDDGSALI
                     LTVANYYTPSGKEIPSEGVAASVPVAPAMDQMTGAPLPPPAQTSSPDDPTVKKALQVL
                     QGTAPAQKAA"
     gene            complement(7443..>7725)
                     /locus_tag="VN788_02340"
     CDS             complement(7443..>7725)
                     /locus_tag="VN788_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXU19403.1"
                     /translation="ERELPELLAFFELPQPLWRKLRTTNAIERCFVEVRRRTRPMVLF
                     VNVASVERIIYAIFHSFNQQWQIAPSPFLHKLLDVTGGHARRSEHQVLL"
BASE COUNT         1548 a         2307 c         2082 g         1788 t
ORIGIN      
        1 aatggtgcga acctggcgag cacggcaagc gctttgccac agggtcccga agtgccgtta
       61 tctcggacaa attcctcagc agtagccagc acccataatg cgatttcgtc cgatagcgcc
      121 accgatcaca atcctcaagc gctggctgcc gaggcgcgaa gcgtacaagc tgcacccact
      181 catgcgatga gcacgcctcc gcgcgtgacg aacgattctc ctgcctccgc ccggaccgtg
      241 gcttcgattc cgtcacgtag cctacagtct gaacggcaag cagcccccgg gggccagcca
      301 acatatacac agtccttcga tccgtctcca tttgatggcg ccgcgcaggc gcttacatca
      361 tccgcacaac cggctcatcc ggaaggaggc ggaattgtcg cagaccggtt cgagaatgcg
      421 ccccacgccg cgtcagctcc gcagaccgca gaaacaaccg tgccatctga agttgctgcc
      481 gacgcaccgg agacccgagt gcttcctggc gggcccccgg gtgcgtccgt gggagcgacc
      541 gtcggatctc ccggccatat cgaatcgtct caactcattc attcggtgca gcctgtctac
      601 ccgctgacag caaaacaact ccacgtggaa ggcgatgtcg agctgcgtgt tgttgttggt
      661 gtagacggga cggtccgcag cgtcggcttg gtaagcgggc ccccatcgtt ggttatggct
      721 gctatcgatg ccgcccgcca gttcctctat aaaccggccc tgctcaatgg aaaggccatc
      781 gagacagtgc aaaccataga aatgtcattc aaattgaagg actgatctca tcccaattcc
      841 gttaaaccgc agcaaggcaa agcaagctac aatgcctgga taaactagac ggaggggtag
      901 gaaagcggtt gactccggcg gtcttgaaaa ccgttaggcc cgaaagggtc tcgtgggttc
      961 gaatcccacc ccctccgcca gatttggttt gtacaatccg aggacatcct ttacagaacg
     1021 ttccgaagac atactttacg ccttcgggcc aaaagggtta ttgagtggct gaagggtttt
     1081 ttcctccaga tcgatgtaac cgagatcatc cataaaagtc accaaccaga tacgatcgtc
     1141 gacctccttg attccgacgg cgatcgagcg ttttttccat ggcgtcggcg gctcctgacc
     1201 ggctagtcca gtcaggagtt gtagaccatg tctccggaac gtttccataa aggatgaggg
     1261 cgaacgcact acgtggttcc cctgcatcag cgcacgcgat tgttttacaa gccctcattc
     1321 gtaacattcc gcggctccgg cacgtatgct agattgatcc cacatggata cccctgcgat
     1381 ctcgacgaca aatctcacgc gccggttcgg cgatttcgtg gccgttgact cgataaacct
     1441 gcgagtcacg cccggacagt tcttcggttt tcttggcccg aatggtgccg gcaagtcgac
     1501 caccataaaa atgctcacgg gtcttctcgc gccgtccgcc ggacgaatgg agattctcgg
     1561 actcgatctt gcatcgcatt ctcttgaagt gaagcagcac atcggagtcg tacccgaggg
     1621 gatggccctc ttcggacgcc tcacaggtcc cgaatatctg aattttgcgg gtcgcatgta
     1681 tggactcaac cgcgaaaccg ccgccaagcg caccactgaa ttgctcgaat tcatgcagct
     1741 tgatcgcgag cccaaaaaac tcatcaccga ttattcccat ggaatgcaaa aaaagctcgc
     1801 gctggccgcc gctgtaattc acggccccaa aatcctcttt ctcgatgagc cgttcgaagg
     1861 cgttgacgcg atcgcatccg gtactctgaa agcgatgctc cagcgcatga ttctccgcgg
     1921 cgctaccatc tttctcacct cgcatgtact cgaaatcgtc gaacgccttt gcagccatct
     1981 cgcgatcatt catcaaggca agctcgtggc ccagggctcg ctcgaagagt tgcgcagcgg
     2041 tgttcaagcg atgcctgcag atcaacatct tgccggtgcg cagcctgaaa agcttacgct
     2101 ggaagaaata ttcctgcgca tcgtgggcgg cgctcgctcg gaggatcagg agctttcgtg
     2161 gctgacgtag ctccaaccgg cgtgccgctc gctctgcccg acgccggcat gcaacttcgc
     2221 acaatcgccc ttctccgctg gcagatcttc aagaactctc tcaggacgat gcggggcagg
     2281 ctggaagccg tctcatggat ttttgtcggc ttctggttcg tagctctggg cctgggtggt
     2341 gcgttcggaa tcgccgcggc ttcttggtgg atggtttctc ataatcgcag cgatctgctc
     2401 gccgcgctgt tttggccaat attcattttc tggatttgct ttccgctggt tgccactgca
     2461 ttcacggaag tgttcgattc ctcgaatttg ctccgctatc ctctgcgcta ctcttcgttc
     2521 tttcttgtca accttatcta cggctcgctc gacggcggca cggtcgtggg tctcttatgg
     2581 ttaatcggcg ccgtcctcgg catctcaatc gctcagccat cgtccacccc atgggctgtt
     2641 ttggccctcg caatcttcgg cgccgccaac attttccttg ttcgtgccct tttctcttgg
     2701 attgaacgct ggctcgccca gcgcaaaaca cgcgaaatca tgggcattat tttcttcctc
     2761 atcattatcg gtttccaatt catcggcccg ctcacgcaac gcgcccatag acaacacttt
     2821 caactgccag cctacatttc ccaggcaatc ctgattcaaa aatttctgcc gcccggtctg
     2881 gtcgcaggag cattaggcgg ttctgttcgc gctgactggt tatccgcagc cggttcgctg
     2941 ttgttgctct ccgcttatgc cggtgttttc tgtcttctgt tccacatccg tttgcgcggg
     3001 ctgtacgcag gagaaagttt tagcgaatcc gtctctcgcg aaaaggtgag catggaaaag
     3061 gccgagaccc aatccggatg ggctttgccg ggtctctcgg gccaactcgc cgccattatg
     3121 gaaaaggaag cgcgttacct ctcgcgcagc ggcccaatgc tctttgcttt gatcatgcct
     3181 atcgttgtgc tgctgctgtt ccacatgggc ggcaatccca accgcccgaa cgccgttgct
     3241 cacaatccca atctcgcctt cccgattggc gcggcctact gtctgttgct tcttacgaat
     3301 ctgatctaca acaacttcgg agccgacggc atgggcgtcc agtttttctt tgtcgcccca
     3361 gtaaagatgc gctcggtcat aatcgcaaaa aatcttatcc atggcggcgt tctcgccctg
     3421 gaaatgtgcc tcgtgttcgt ggctacttat ttcatgtacg gatcacctgc ccccgctgtg
     3481 gtcagcgcca cattcgtagg tgtgctgttc gcgattcccc ttgatttcgc ggtcggcaat
     3541 ctgctttcgc tctattcacc caaaaaatat gactatggtg ccttcggccg tcagcgcgcg
     3601 ccaggtctca cagtccttgc cagtttcggt gtgcaggcgg tcacgattgg tatcgccatc
     3661 gtcgtggtgc ttgtcgcgcg tcattatggg agcctctggc tggccacgat cgtatttgcg
     3721 gctttagctc tggctgcatg ggctctatat caagccgtgc tcggccgcat cgacggcatc
     3781 gcattgtcca aacgcgaatc gctcatcgcc acaatcgcga aaacaggttg aatcctcctg
     3841 acctgtcggg cttcggcgaa ggctttgcta taatcgctcg ccatgcccgg tccagctttc
     3901 cgagcgccac gctatatcgc tatcgaaggg cccatccggg tcggcaaatc cacgctggcg
     3961 cgcattctgg ctgaacagtt gcacgcacgc aggatcttcg attccgaaga taatcccttt
     4021 ctggcgcagt tctacgacga gaaacccggc gcagcttttc gcgcccagat gcatttcctt
     4081 tacgaacgtt atcgccgcct cgccgacgct gccgcagatg aaaaagcagg tccactgctg
     4141 agcgacttca ttttcgaaaa ggacaaaatt ttcgcctaca tcaacctcga caacgaagaa
     4201 cttcgcctct atgaaaagta tttcgatctt ctctcgccct ccttgcctgt tcccgacctt
     4261 gtcatctatc tccaggccaa ggcggaagtc ctgcgcaagc gggtttcgaa gaaaggcgat
     4321 ccatccgaaa cggaaatatc cgcggaatac ctcgaagccg tcgccaatgc ctacgaacac
     4381 ttcttctttc gctactccgc ttcgaatctt ctagtagtgg atacttcgga aatcgacttt
     4441 gttgagcaca accaggattt gcaggagttg ctgaaccgtc tgcgccagcc ggtgaagggg
     4501 acgcagtatt ttcttcctct tggccaaggc tagttttcac ttcgtatcgg cgcatcgaaa
     4561 agaaaatccg cgccggtgcg cgaagttggc gcagtccggc gcggttatga ttctgctgtt
     4621 tacgcagctt tcgacggccc agacatgcga tgccgcagct tgcccagcct tcggtttaac
     4681 ggatggccgc gatcgagaaa cgagaaaacc ggctcgcgtc gcgtcccgat tttctccaac
     4741 gcgcgccgaa tccggaaatc ccgccccgct gagatcatcc ccttctcgag tgtttggatg
     4801 gcatcgggtg tgcggcctgc ctgcacgtaa acctcggcca gattcagata gagttgagca
     4861 tggttgtgcc gcagccccag tgcttcccga cacaacgact ccccatccac gaagcggcgt
     4921 tctgctatcg cggcaagcaa tcctgtataa gaaaggtaga acggattctt gggggccgtt
     4981 ccgagggcgc gtcgagcgtg gaggagagcc tcaccggctt ctccgtctct tagaagtttg
     5041 aggcattctt tgaagtccgt catcccggca tccacgggtg atgccgctcc aatccagtgg
     5101 gacatattca ccttccttac ccttgagcgt gttacgtaac gccgcgcgtc acgtgcatga
     5161 agattattaa tgacggggtt gcctgcggga aataggacgt acgaacagat ttcgtcccag
     5221 tacgaatcac tgtacacaat gacaggcgaa gacatggaga ggacgctcgc agtgccttag
     5281 tacgggaatt tagtgcgcca aatccgacgc gaatacgagt tgaattccat cttccttcag
     5341 ccgcggaagc atctggcgta gcgtcgcgat cgtctccgga tggggatggc cgatggcaat
     5401 cgcttgcccg tcgcgatcgg catcctttat tgcgaggtcg atctgcttct caatcgcatt
     5461 cgcattgagc gcatcgtcca gaaacacctt ccgggacgca gcgcgaacgc catagcgtcg
     5521 cgcggtattg tatgcaaccg tcgaggccgt cgtccgactg tcgacgaaaa agagcccacg
     5581 ctcgcgaagc gcaggcatta gcgcatccat cagctgtggg tcggccgtag ccttggatcc
     5641 ttcgtgatta ttcactcccg cggcgtgagg cacggtctcc agcatcccgg cgaacacgga
     5701 ccggacctct ttggcggaca tgccggggcg cagttcgaca gcttcgtgcg ccgtgctttc
     5761 agaatcgggc tgcatcggca cgtgcagcat tacttcgtcg cctcggcgag cagcctcgtc
     5821 agcaatcgtg gtggagaggg gtagatgcgg aagcacggag accgttacag gatagggcag
     5881 ggcgatcacg gcgtcgtctt cagcacggtc gttgcccata tcgtcgatga tgattgctaa
     5941 ccgccgtgca ctgcggaaag ccggctcgcg atgagccgaa cgggtctcgc tctcttcctt
     6001 cttagcgcga gctttgcgcg cgggtttctt cgaacacccg gcaagcagaa gggctaccac
     6061 ggcgcatccg cacatcaagc ccgtagaaag ccctctaaac agcagaaaat gataggtttg
     6121 tgagattctg cgcttgcggg gatacacaga tgccaagcga gcgagccgct caggctgcct
     6181 tttgcgccgg agcggtgcct tgcaaaacct gcaaggcctt tttcaccgtc gggtcgtccg
     6241 gtgaagatgt ttgcgcgggc ggcggcagcg gtgctcccgt catctgatcc attgctggcg
     6301 cgactggcac gcttgccgcg acaccttccg acggaatttc tttgccgctt ggggtgtagt
     6361 agttggcaac cgtcagaatc aatgccgatc catcatccat cgggatcagc ttctgcatgg
     6421 aggctttgcc gaacgtgcgg tcgccgatcg tctgcccacg tttgttatct gagatcgcgg
     6481 ccgcgacgat ttcagccgga cccgccgtcg catttccgat cagaactgtt acaggcccat
     6541 tccacacaac cttggagggg tcggccgacg aaactaccgg gctgacggtt tggcctttca
     6601 gggtcgtaat tgtcccagag gggacgaaga gttgcgcggt ggcgatgccc tcggcgtctt
     6661 ctcccagcga gcagttgcgg aggtcgagga tcaacttttg ctcgccttcg tgactcattt
     6721 gcaggagatc gtcgtgaatt tctttggtgg tgctctcgtc gaactccggc acaccgatat
     6781 aaccgacgtt atcctgaagc tttgcctccg tcactttcgg gggtgcaatc ttcgcaagcg
     6841 tgatgttcat ctcctgcggc tccggctttg cccgggtgat tacggagagc tttaccgttg
     6901 ctcccggatc gccgctcagg agaagtctcg cttgctcgat ggacatttgc tctgtgttga
     6961 agtcgccgat cttctcaagc acgtccgcgt attggagtcc caatttatcg gcagggctgc
     7021 cgggcagcac cgagaggaca ctgatgtatc cggcgcgaat tttcgtcagc gccatacccg
     7081 ccgtagcctt gtcctttacg aggatgcgct gcttgtaatc cttatattcc agcgggctca
     7141 tgtagctcga ttccgcatcg agcgaatcga gcagcccgtg aagcgcgcct gaagtcactt
     7201 tgggaatatt gggttcgtcg acataatcgc gctgaatccg gtccatcacc tcgctataga
     7261 ccgtgagggc ctgataggcc ttatcgtccg aagaatgcgc gagtacataa cccacgccaa
     7321 cgtaggcaaa cgcgacgacc gtgagcacca cgacaatcgc cttcgaaaga ttgctcatgc
     7381 caatattcct ttacgcccct gcgcttcttc cgcccatatc ccaatcgcga agcgaactcc
     7441 gattatagca gaacctgatg ctcagaacgg cgtgcatggc caccggtgac gtcaagcagc
     7501 ttgtgtaaaa agggagaggg cgcgatttgc cactgctggt tgaagctgtg aaagatggca
     7561 taaataatcc gctcgacact ggcgacgttg acgaacaaga ccatcggccg ggtgcggcgc
     7621 ctgacctcga cgaagcagcg ctcgatggca ttggtggtgc gcaacttgcg ccacagtggc
     7681 tggggtaatt caaagaacgc tagcagttca ggcagttcgc gctcc
//
LOCUS       DATNDT010000078         7261 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_922126, whole genome shotgun sequence.
ACCESSION   DATNDT010000078 DATNDT010000000
VERSION     DATNDT010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922126"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            506..2797
                     /locus_tag="VN788_02345"
     CDS             506..2797
                     /locus_tag="VN788_02345"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU19404.1"
                     /translation="MRKAYVFLFAAALLAFGQPARATVFGGVRGVVHDPQHRPIPGAQ
                     VQLKSATSDWSQQTQTNQDGEFAFATLPLGDYIVNITASGFESARQSVTVTSDSSPVL
                     HFPLAIAAVNQTATVSAQAPVGNVESATPTTLLDRNDIALTPGADRTNSLAIVTDFVP
                     GAYLTHDQLHIRGGHQVSWLIDGVPIPNTNIASNVGPQFDPKDIDYLEVQRGSYEADT
                     GDRTYAAFNVVPRNGFERNNEAQLITSFGNFYQTNDEFNLGGHTPRFAYYVSVNANRS
                     NLGLETPIAKIHHDAENGVGGFGSLIFNAAPKDQLRFVTSLRRDYYQIPYDPDPNDFE
                     NQQFQTSGLRDGQHEADDFVVFSWVHTFNPNAIVTISPFYHFNRANYGGSPNDTPAQT
                     TDDRSSNYVGGQATVALTLPKNNLQVGTYTFAEHESEFFGLLLNPRPTPAPLANPIRD
                     SETSSGALEAFFVSDKFSVAPWLTLIGGFRATYFSGSITENTIDPRLGAALRVPYLRW
                     VFRAYYGRYYQAPPLASITGPLLSEINSLGGIAFTPLHGERDEEHQFGVTIPVRGWSL
                     DIDDFETRARNFFDHNNVGESNIFIPVTIAEARIRGLEVTIHSPRLWNRGEVHLAYSN
                     QMAQAAGAITGGLICFPPTDPSCLPEPGFSALDHDQRNTLNVGFDANLPWRLFAAANV
                     YYGSGFSNGSPNAQFPDPYLPGHASIDASLRKDLGERFSVAVNALNIADRHLLIDNSL
                     TFGGFHYNDPREVYVELRYKFHY"
     gene            2852..3808
                     /locus_tag="VN788_02350"
     CDS             2852..3808
                     /locus_tag="VN788_02350"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXU19405.1"
                     /translation="MRLESSSARSHRQARTTLLIVTAAFALIFAACKRAPAPQAKRYH
                     LVGTVISVDTAHGSANIDGQEVPGFMAAMAMPYSVHNSKALAALSPGDQITADIVVTD
                     TDSYLDNIVVTKKGANPANTTGPTHLPQPGEKVPDFALTNQNGKRIHLRSYQGDVLLV
                     TFIYTRCPFPTFCPLVSRNFAQIYAQLRKDPAIGSKKIRLLSVSFDPKHDTPTVLKRY
                     AETFSSTTGGNPFDRWEFAAVPAKELPKVANFFGLYYSASRDQIVHSLSTSVISPDGT
                     IYKWYQDNTWKPTDLLQDARQSLEQDNRRNAGPRSTTSVPTA"
     gene            complement(3827..4843)
                     /locus_tag="VN788_02355"
     CDS             complement(3827..4843)
                     /locus_tag="VN788_02355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HXU19406.1"
                     /translation="MAFAVQIAKGIFDLVRDDLALVEQEIAAQNSAAIEPVEQIASYL
                     YQGGGKRLRPALLLLAAGAAGYRGESAIRLGAVVEMIHSATLVHDDVIDGAQMRRGRP
                     STNARWGNHMSVLAGDWLYMRSFEMALRERNFSILDILIGLTQNMVEGELLQLTLLGR
                     MDVTETDATELAYRKTACLFSGCARLGAVLGRQPKHVEESLAEYGRNAGLAFQLVDDL
                     LDFTASPEQLGKPVLSDLKEGKVTLPLIFSLQNGASQSGAAGFHREARQLVAKVLEER
                     DFLSVPPEQITRLVMETGALDRASNLARDYVRRAKMCLEELPDTEYRRALLAVPDFIL
                     DRQS"
     gene            complement(4863..5672)
                     /locus_tag="VN788_02360"
     CDS             complement(4863..5672)
                     /locus_tag="VN788_02360"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HXU19407.1"
                     /translation="METIDSHAHLEFAQFDGDRDAMLDRARAAGVQTLLAIGSGTGPL
                     ERLDAAIPFAEQHDWIYATVGIHPHDASAATDEHFVKLEELARHPRVIALGELGLDYY
                     YDHSPRDVQQQVFRRQLDRAAAAKLPVVIHCRDAWDDCLAILDQDWRPTGLGGIFHCF
                     TGTLEEARRGMDMGFMISFAGNVTYPKMQHLRDVSREIPLEMILTETDSPFLPPQTQR
                     GKRNEPAFVVQVAEALANVRDLAPEDVAATAAANFRRFFRLSAAASPAEPR"
     gene            complement(6260..>7261)
                     /gene="metG"
                     /locus_tag="VN788_02365"
     CDS             complement(6260..>7261)
                     /gene="metG"
                     /locus_tag="VN788_02365"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HXU19408.1"
                     /translation="GNFSRESLIIRYNADLANGLGNLASRVLTMMEQYFDGRIPNMPV
                     PWPGEREKQIENLKTWVFEAARARYDSFDFSGALEAIWRLIAEVDKYLVEFQPWVLAK
                     DKAKWDQLETVLSAASDAVRVATFLAHPVLPESTQRIWPQLGQVGKLGSEFLDDLLLP
                     LGRQPVRIGKPEGVFPRADKKELYERIEAMENEIRNPGTAQPSATSPAPAATGASAAS
                     STAPAASSKITIEDFAKVELRVGRIDSAERIQGADKLLKIMVNIGDEVRQVLAGIALA
                     YAPENLVGRKVVVVTNLAPRKMRGLESNGMLLAASTADGNPVLCTFAEEIPPGSKVK"
BASE COUNT         1502 a         2148 c         1995 g         1616 t
ORIGIN      
        1 tttgaacggc agcataattt cgccagccta cctcgaaccc tctgccgacg caaaccccac
       61 aaaactggtg tagtcatacc gcattcgcgt ctgcaacatc gctgtggtag gctcgcagag
      121 atgcaacgcg ccaaagttac catcgttatc gttgctcttt tggcggtgcc tctggcgttg
      181 ctcgcccgcg gcatggcttg cgaatcgtct tccggcccca gggtttgccg catgctctgt
      241 ggcccgcaac atgggaatca gccgatggtg tgccactgtg ccgaaaaatc ttcgaagcat
      301 attcctgact tcggtttgat agctcctctc ccgcctaccg agcccgaagc ttttgtcgtc
      361 ctcgcggccc cagactctct gaggcagccc atgcattcgc aatctcaggt ttcacgcccc
      421 ggatttgtgc cagtgccgtt cgaacccccc aaagcctaac cgcccctcac cgcaatccag
      481 aaagcaaatt ggaacggaga agttcatgcg gaaggcttac gtgtttttgt ttgccgcggc
      541 gctgctcgcc tttggacagc ctgcacgagc aacggttttt ggcggagtgc gcggcgtcgt
      601 ccacgacccg cagcatcgtc cgattcccgg cgcccaagtg cagctaaaat cggccacttc
      661 ggattggtcc cagcagacgc agaccaacca ggatggcgag tttgccttcg ccacgctgcc
      721 gctcggcgat tacatcgtca acatcacggc ctcgggcttt gaaagtgcgc gccagagcgt
      781 cacggtcacg tcggattctt cgcccgttct ccattttcct ctcgcgattg cggcggtaaa
      841 ccaaacagcg acggtttccg cacaagcccc ggtgggaaat gtagagtcgg cgacgccgac
      901 gacgcttctg gataggaacg acattgccct cactcccggc gcggaccgca caaacagcct
      961 cgctatcgtc accgacttcg ttcccggcgc ttacctcacg cacgatcagt tgcacatccg
     1021 cggcggccat caggtgagct ggctgattga cggcgtgccc atcccaaata ccaatatcgc
     1081 cagtaatgtg ggcccgcaat tcgatcccaa ggatattgat tatctcgaag tccagcgcgg
     1141 cagctatgag gccgataccg gcgaccgcac atatgcagct ttcaacgtgg tccccagaaa
     1201 tggctttgag cggaataacg aagcgcagct cattaccagc ttcggtaatt tttatcagac
     1261 caatgacgaa tttaatctgg gcggtcacac tccacgcttc gcctactacg tcagcgtcaa
     1321 cgcaaaccgc agcaacctgg gtctggaaac tcccatcgcg aaaatccatc acgacgcgga
     1381 aaacggcgtc ggcggcttcg gctcgttgat cttcaacgcg gctcccaagg atcaactgcg
     1441 cttcgtaact tcattgcggc gcgattacta tcaaattccg tacgaccctg atcccaacga
     1501 ttttgaaaat cagcaattcc aaaccagcgg cctgcgcgat ggccagcacg aagcggatga
     1561 cttcgtggtc ttctcttggg tccacacttt caatcccaat gcgattgtca ccatttcgcc
     1621 gttttaccac ttcaaccgcg cgaactacgg cggctcgcca aacgacactc cggctcaaac
     1681 cacggatgat cgctcttcga actacgtggg cggacaggca accgtcgcgc tgacactccc
     1741 caagaataac ttgcaggtcg gcacctatac cttcgcggag catgaaagcg aattcttcgg
     1801 cttgctactc aatccccggc ccactccagc gccacttgcg aatcccatca gggattccga
     1861 gacgtcttcc ggcgcgctcg aagctttttt cgtcagcgat aaattcagcg tcgcgccatg
     1921 gctcacgctg attggcggat tccgggccac ttatttctcc ggcagcatca ccgaaaatac
     1981 aattgaccca cggctcggag ccgccctgcg cgtgccgtat cttcgctggg tttttcgcgc
     2041 ttactacggg cgttactacc aagcgccgcc gctcgctagc atcacgggac ccctgctctc
     2101 cgaaatcaac agcttaggcg gcattgcctt tactccactc cacggagagc gcgacgaaga
     2161 acaccaattt ggcgtcacca ttccggttcg cggctggtct ctcgacatcg acgatttcga
     2221 aacccgtgcc agaaatttct tcgatcacaa taacgtcggc gaatccaaca tttttattcc
     2281 cgtcaccatc gccgaagccc gaattcgtgg cctcgaagtc acgatccact cgccgcgcct
     2341 ttggaatcgc ggagaagtcc acctcgccta ttcgaaccag atggcgcagg cggcgggagc
     2401 aattaccggc gggttgatct gctttcctcc caccgatcca agctgcttgc ccgagccagg
     2461 attttcggcc cttgatcatg accagcggaa cacactaaac gtgggtttcg atgcgaacct
     2521 tccctggcgg ttgttcgcag cggcgaatgt ctactacggt tcgggattca gcaatggctc
     2581 gccgaacgct caatttccgg atccgtatct tcccggacat gcttcgatcg atgcttcgct
     2641 gcggaaagat ctcggcgaga ggttttctgt tgccgtaaac gcgctgaaca tcgcggaccg
     2701 tcacctgctg atcgacaaca gcttgacctt cggcggattc cattacaacg atccgcgcga
     2761 ggtttacgtc gaacttcgtt acaagtttca ttactgatgt aatgcgcttc attgcgggcg
     2821 tacaatgaga aattcaaaag gagtgaaaag agtgagactt gaatcaagca gtgctcgcag
     2881 tcatcggcaa gctcgaacca ctttgttaat cgtaacggct gccttcgccc tcatttttgc
     2941 agcgtgcaaa agagcgcccg cgccacaagc gaaacgctac catctcgtcg gcacggtcat
     3001 ctcggtggac accgctcacg gctccgcgaa tattgacggt caagaagtcc ctggatttat
     3061 ggccgcgatg gccatgcctt attcagtgca caattccaag gccctcgcgg cattgtcgcc
     3121 gggtgaccaa attactgccg acatcgttgt cacagatacc gactcctacc ttgacaatat
     3181 cgttgtcact aaaaaaggcg cgaatcctgc gaacactacg ggtcctaccc atttgccgca
     3241 gcccggcgaa aaggtccccg atttcgcgct cacaaaccaa aatgggaaac gcattcacct
     3301 caggtcgtat cagggcgacg ttctccttgt tacttttatt tatactcgat gtccttttcc
     3361 cactttttgc ccgctggttt ccaggaactt cgcgcagatt tacgcgcagc ttcgcaaaga
     3421 tccagctatc ggttcaaaaa agattcgcct gttaagtgtg agtttcgacc cgaaacacga
     3481 tacgcccaca gtcctgaagc gctacgcgga aacgttcagc agcaccacgg gtggcaatcc
     3541 attcgatcga tgggaattcg ctgctgtccc cgccaaggaa cttccgaagg tcgcgaattt
     3601 cttcgggctg tactacagcg catcccgcga tcagatcgtc cactcgttga gcaccagcgt
     3661 catttcgccc gatggcacga tctataagtg gtaccaggac aatacttgga agccgaccga
     3721 tcttttgcag gatgcgcggc aatcgttgga gcaagataat cggcgcaacg cgggaccacg
     3781 cagcaccaca agcgtcccca cagcataaag tcacatcatt gtgacgctag ctctggcgat
     3841 cgaggatgaa gtcggggacg gcgagcaaag cccggcggta ctcggtatcc ggaagttctt
     3901 cgaggcacat tttggctcgc ctcacgtagt ctcgcgcgag attggatgcg cgatcgagcg
     3961 cgcccgtttc cattacaagt cgagtgatct gctccggagg aacactcaag aaatcccgtt
     4021 cctccagcac cttggccacg agctgacgcg cttctcggtg gaagcccgca gccccgcttt
     4081 gcgaggcacc gttctgtaga gaaaagatta gcggcagcgt gacttttcct tcttttaagt
     4141 cgctcagcac aggttttcct aactgctcgg gtgatgccgt gaaatcgagt aaatcgtcta
     4201 caagctgaaa ggccaaaccg gcattgcggc catattccgc cagagactct tccacatgct
     4261 tcggctggcg gccgaggact gctcccaatc gggcgcatcc gctgaaaagg catgcggttt
     4321 tgcgataggc cagttcggtg gcatccgttt cggtcacatc catacggcca agcagcgtca
     4381 attgcaggag ttcgccctcc accatgtttt gggtcaaacc gatcaagata tcgagaattg
     4441 agaagtttcg ttcgcggagt gccatctcga acgaccgcat gtagagccag tcgcccgcca
     4501 acacgctcat atgatttccc cagcgcgcgt tggtgctggg tcggccgcgg cgcatctgcg
     4561 cgccgtcaat cacgtcgtcg tgaaccagcg tggcgctgtg aatcatttcc accacggcgc
     4621 ccaggcggat ggcgctctca ccgcgatatc cagccgcgcc ggctgccagc aggagcaggg
     4681 ctgggcgcag ccgctttccg ccgccctggt acagatagct ggcgatttgc tctaccggtt
     4741 cgatggcggc gctattctgg gcggcaatct cctgttcgac gagcgcaaga tcgtcgcgca
     4801 ccagatcgaa aatcccttta gcgatttgga cagcgaaagc cattccgtat gatccttcag
     4861 ccttaacgag gctcggcggg agaggctgcc gcgctaagcc ggaaaaaccg tcgaaaattt
     4921 gctgcggctg ttgccgctac gtcctcgggc gctaagtctc tcacattcgc caaggcttcc
     4981 gctacctgaa ccacaaaggc aggctcgttg cgcttgccgc gttgtgtttg cgggggcaaa
     5041 aagggcgaat cggtttccgt aagtatcatt tccagcggga tttcgcgcga aacgtcgcga
     5101 aggtgctgca tttttggata ggtcacattc cctgcgaacg aaatcatgaa ccccatgtcc
     5161 attccgcgcc gcgcctcttc aagggttccc gtaaagcagt gaaaaattcc accgagaccc
     5221 gtcggccgcc agtcttggtc gagaattgcg aggcagtcgt cccaagcgtc gcggcaatgg
     5281 attacgacgg gcagtttcgc ggcggcggcc cggtcaagtt gccgccggaa aacctgttgc
     5341 tgcacgtcgc ggggcgaatg atcatagtag tagtcgaggc ctaattcgcc gagggcaatc
     5401 acgcgcggat gccgggccag ttcttcgagt ttaacgaagt gctcgtcggt tgcggcgctg
     5461 gcatcgtggg gatggatgcc gactgtggcg taaatccagt cgtgctgctc ggcgaaggga
     5521 atcgcggcgt cgaggcgttc aaggggcccg gtgccgctgc cgatggccag cagtgtctgg
     5581 acgccggccg cgcgggctcg gtcgagcatt gcgtcccggt cgccatcaaa ttgggcgaat
     5641 tcgaggtgcg cgtgtgagtc gattgtttcc atgattagtc tcagttagct cttttcaccc
     5701 ttctaaaact gtgacgggta gaatgggtgc ccgtcctttt ttttcaataa gatggagcat
     5761 gttctactcg gccgtagtgt cgaatagagt tgcaagccgc tcggcttgtg cagccagcac
     5821 ccggatcgta tcggccaccg catagcggct cgttggcgtt gtctttcggc cggatttttt
     5881 cgaccacttt gatcatgcgg tcaggttatc aggataggga tgccttgcaa ggacgagttt
     5941 gtgtggactt tggaggcgcg cattcgcgaa tgggcatgga gatgatggga aacttgaaac
     6001 acactacttg ctgcgggtga agccgcgcag cggaaggcgc ttcgcgccgg tcgttatgtc
     6061 agggctaaag ctctgacctt ccaccgaagt agaggcgctt cgcgcaagag caaagtcgcc
     6121 cgcctgaaag gcgaggcggc cgctacaaag gcaaagacgc aggacactcc gctcccttcc
     6181 tttgtcaggg caagcgggat ggcgatctcc ggtatgtctg gaatgcggtg gactcccaac
     6241 accggacctg atcggatcgt catttcactt tggagccggg gggaatttcc tcggcgaagg
     6301 tgcagaggac ggggttgccg tcggcggtgg acgcggcgag gagcatgcca tttgattcta
     6361 ggccgcgcat ttttcgcgga gctaggtttg tgacgacgac gactttgcgt ccgaccaggt
     6421 tttcaggggc gtaggctagg gcgattccgg cgaggacctg gcggacttcg tcgccaatat
     6481 tgaccataat tttcaggagt ttgtccgcgc cttggattcg ctcggcggaa tcgatgcggc
     6541 cgacacggag ctcgaccttg gcgaaatcct cgatcgtaat tttagaactt gccgcgggcg
     6601 cggttgatga cgctgcgctc gcgcctgtgg ctgctggagc gggagaggta gccgaaggct
     6661 gcgcggtccc gggattccgt atttcgtttt ccattgcttc gatcctttcg tatagctctt
     6721 ttttgtccgc gcgtggaaac accccctctg gtttcccgat tctgaccggc tgacgcccga
     6781 ggggtaacaa taagtcatca aggaattcgc ttccaagttt gccaacttga cctaattgag
     6841 gccagattcg ctgggtcgat tctggtagga cggggtgcgc aaggaaagta gctactcgga
     6901 cggcatcgga ggcggccgag agcaccgtct ccagctggtc ccatttcgct ttgtcctttg
     6961 ccaataccca gggctgaaat tcgaccagat acttatcgac ctcggctatt aatcgccaaa
     7021 ttgcctcaag cgccccggag aagtcgaaac tgtcgtagcg agcgcgagct gcttcaaaaa
     7081 cccaggtctt cagattctcg atctgcttct ctcgctcgcc aggccagggg actggcatgt
     7141 ttggaattct gccgtcaaaa tattgctcca tcattgtcag gacgcggctg gcgaggttgc
     7201 ctaggccgtt ggccaggtcg gcgttgtagc ggatgatgag ggattcgcgg gagaagttgc
     7261 c
//
LOCUS       DATNDT010000079        10147 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2071362, whole genome shotgun sequence.
ACCESSION   DATNDT010000079 DATNDT010000000
VERSION     DATNDT010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10147
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071362"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="VN788_02370"
     CDS             <1..528
                     /locus_tag="VN788_02370"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU19409.1"
                     /translation="VATEDGKTVEVGVIGNEGFAGSVLAVGLRRSSLREIVQIAGEGL
                     RIEASALHAVIPIVPQLQLNLSRYAVLLGMQVAQTAACNRLHTVEQRLARWLLVTQDR
                     VDAGMLPLTHDFLATMLGTDRPSVSLAAASFQRKQILQYGRGAVTILNRKELERLSCE
                     CYGQIQQFNGELGLR"
     gene            622..1374
                     /locus_tag="VN788_02375"
     CDS             622..1374
                     /locus_tag="VN788_02375"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU19410.1"
                     /translation="MHAKGRLAAVEPSERRDLDGRAIRNKILISLPDTEYRAARHHLE
                     YVVLPQLSVLHEPSEKLEFAYFPNAGLISLVVAMENGKTVEAAVLGREGAAGIPSAVH
                     LKHSPLREVVQVAGDGFRVKVSALQPILKSAPEFQMTLSRYAIIQGIQVAQTAACNRL
                     HDVEQRLARWLLMAQDRVDNAPVTITHDFLSTMLGTDRPSVSLAAGVLQRKRYIEYTR
                     GAVKVLNRKKLESSACECYGVIRQYNAEIGVR"
     gene            1484..1687
                     /locus_tag="VN788_02380"
     CDS             1484..1687
                     /locus_tag="VN788_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19411.1"
                     /translation="MDRIYDIFRKMPDNSPIWVESVDGLEALKERLLYLASTKPNEYL
                     VYDHGLQRFIDPFDNPREFLQAK"
     gene            1812..4004
                     /locus_tag="VN788_02385"
     CDS             1812..4004
                     /locus_tag="VN788_02385"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXU19412.1"
                     /translation="MRKPSISLSARRTVDLRFSELTDKVRRNRPGDDLDLLRRAYEFA
                     AEQHKTQTRRSGEPFLSHPLEVAHLLADIKLDVTSLCAALLHDVVEDTKIPVEKIAEQ
                     FGPDVARLVEGVTKISKLDLVAPEDRQAENVRKMLLAMVNDVRVVMVKLADRLHNMRT
                     LEYLTPESQQRIARETLDIYAPVANRLGMGLIRGELEDLAFRYIETDAFLELQKKVAS
                     KQNVFDKFLDEVQETIREKMIENGIPAEVQARIKRLYSVHLKIQKQQRNLDQIYDLLA
                     VRVVTDTVKNCYAALGVIHQIWRPVPGRFKDYIAMPRPNLYQSLHTAVIHSGQPFEVQ
                     IRTQEMHRIAEQGVAAHWKYKGGPEVASSADDQRIVWMRHLIEWVQEMQEPSEFLSTL
                     RVDLYPEEVYTFTPKGRVVVLPRGATPVDFAYAVHTEVGHQCSGAKVNGEMVPLRHAL
                     TNGDVVEIVTQKGHTPSRDWLSFIHTSRARSKIRQWINLHERQEATDVGRRLLEKEAR
                     QAGVSLKKISSEDLQRVATEYGCSRVDDLYADLGYGKWSARQVIAKATGQPLPANFEE
                     KPPKPASNIRRMLGLDEAAIIVRGHDDLMVYRSKCCNPIPGDDIIGYVTRGRGIAVHS
                     KNCPNVENLLYEADRRIPVEWAGSTHAEFPVQLRIITEDRPGMLASITGIISETGANI
                     RTFESGGEDIRARIEVALDVRDRKQLERILARIKRIPGVFDIERVYNV"
     gene            4081..4728
                     /locus_tag="VN788_02390"
     CDS             4081..4728
                     /locus_tag="VN788_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19413.1"
                     /translation="MGCCSTDAHDAAQTRFSGLILCSLLMSFGLLLSGCHILHPVDTK
                     PLDAVGMSYDAVQELKSLRVSTAEVTEIAKARQSGFSDADCIDAFKVFRSRSQSFDAG
                     DAIASLVEVSVSEDTILELARINQLGLDSGELQAMKLAGLSDDILLEVARHRAHGKPV
                     LSGASLARLKNTGMRASTLFELAQRGVPDWQESAIVSYRRHGASDDQVLRRFAGI"
     gene            complement(4804..4880)
                     /locus_tag="VN788_02395"
     tRNA            complement(4804..4880)
                     /locus_tag="VN788_02395"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4844..4846),aa:Ile,seq:gat)
     gene            5047..6618
                     /locus_tag="VN788_02400"
     CDS             5047..6618
                     /locus_tag="VN788_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU19414.1"
                     /translation="MRRARFSPVALILLLLCCVRVTARQAPQENLAVSTKSIEAPLLK
                     VCLRLEDNTSFGGVMRVRLLPAEGDKISGVPNGSDGETTFANVQPGTYLLEASAPGFV
                     TVRREVRIELGHGLQTLFVTMKPMPFAARLPEASPTSSDAQPLPASLTGGPSPPRAAS
                     RGAALVSWIPPSLDTTVPAVNPSVPCPLPGLLKIAGVRMTEFVANLERFNATEQVEHF
                     KINAAGLRGSADVRSFDYVVLVSRNRSGLFQLEEYRNGTVDPGLFPAGVATLGLPAMA
                     LIFHPFLVSDFDFKCEGLGQLGGRAAWQIHFAQRPDRPSRIREYVIGKNSYPVPIKGR
                     AWIDPGTDQVVRLESELVKPIPEIALTKERLSIEYEPVEFHSNGQRLWLPETAEVYSE
                     RGTVRYYRRHTFSNFKLFTVAADQSIQPKEAYVFTNTSDRDVAGILTVFPVPGSTLQP
                     VSITFTIPAGKTILKSVGVGKDIRVPPVSLGSATFSHNGPEGSITVNAYLTNDSSVDV
                     TPNADISMSTVPIQN"
     gene            6723..7484
                     /locus_tag="VN788_02405"
     CDS             6723..7484
                     /locus_tag="VN788_02405"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19415.1"
                     /translation="MDSSSPKIAIVTGAGSGIGKSVAVALAQNCYSVVLAGRRKAPLE
                     TTALEVRQANSQALVVCADINDPAAVRELFAKTKDAFGRLDLLFNNAGISGPAIPLED
                     LAYEKWKSIVDTNLTGTFLCTQEAFKIMKNQEPRGGRIINNGSISAHAPRPNSAPYTA
                     SKHGVTGLTKATSLDGRKYDIACGQIDIGNASTHMAERMKKGVPQPNGTVAVEPTMDP
                     ADVARAVVYMASLPLDANVQFMTVMATKMPFIGRG"
     gene            complement(7541..9514)
                     /locus_tag="VN788_02410"
     CDS             complement(7541..9514)
                     /locus_tag="VN788_02410"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXU19416.1"
                     /translation="MSEIHEQAWFPKNLRDHVTDALQVILNWGNIYRPVASRLAQAIK
                     AAGADRVVDLCSGAGGPWTWLHRTIADQATAKIGVCLTDKYPNLAAFENAQQESRGAI
                     TYFAEPVDAVRIPAQVAGFRTIFSSFHHFSPKEAAGVLQNAVDRRQGIGIFEAAARHP
                     LNVLLTFLAPVAALLVAPFMRPFRWSRLFWTYVIPVIPFVLLIDGILSCLRAYSTTEL
                     SQLVSSISADGYRWQIGKGAAGIVPVTYMLGYPEVLATSVVDPLPENSNKTSIPSTSS
                     KSSADKGTADVLIAGGGIAGSSLAIMLGRRGLAVQLFERAEFPREKPCGEGLMPGGVA
                     VLDRLKVTDSVGGTRFRGVRYHFNGRTVEGNFPKRVGFGQTGIGQRRKVLDHVLYSEA
                     AATPGVRIRTGCRVEGPSIENGRVAGLIMGGKRVLAPLVVGADGMHSVIRQKLGLSCP
                     PRRGRFGIRAHFRLSENKEQSDRVEIFVGKGKEIYVTPLPDHEINVIALGEVSGTQED
                     RKISLLQRCEAFPELASLLEGANQVSATIGASPLSGRAIAGVAPGVVLLGDAAGFVDP
                     VTGGGMTQALMAAELLAGYIAANELNEDDWLWSFERERQRMLRGYVLLTQAILRLSDK
                     PHLTDVVLSVLGHWPALLSHGLAVSAGTKPLLG"
     gene            complement(9668..>10147)
                     /locus_tag="VN788_02415"
     CDS             complement(9668..>10147)
                     /locus_tag="VN788_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HXU19417.1"
                     /translation="ATAARHGRAANDSQKKDRAAITQTVQNYMDMWNKHDVHAVAMTY
                     TEDCDFVNNFGDPTHGRAGMEATFGPFMTGVYSETHQTGQVRSIRFLKPDVAAVDVDW
                     QMTGAKNQDGSMRPTRKGIHSLIMMKQNDGSWLIAVMHVHEFTVTPRLANPPAARRQ"
BASE COUNT         2211 a         2949 c         2802 g         2185 t
ORIGIN      
        1 gttgccacag aagacggtaa aacagtcgaa gtcggcgtga ttggaaacga gggcttcgcg
       61 ggatccgttc tcgctgttgg cctgagaaga agctcacttc gcgaaatcgt gcagatagcc
      121 ggtgagggtc ttcggataga agcgagtgcc ctccatgccg tgattcccat cgtgccccag
      181 cttcaattga acttgagccg ctacgcggta ttgctcggaa tgcaagtggc gcaaacggca
      241 gcttgcaacc gcctgcatac ggtcgaacag cgtctcgcgc gatggctgct ggtgacgcaa
      301 gaccgggtgg atgcgggcat gcttccgctg actcacgatt ttctggcgac catgctcggg
      361 acagacagac cgagcgtaag ccttgcggcc gcctccttcc agagaaagca aatccttcaa
      421 tacgggcgtg gcgcggtcac tattctgaat cgcaaagaac ttgaacggct gtcctgcgaa
      481 tgttacggtc agattcagca atttaacggc gagctcggcc tcagataacc tcgtttaatt
      541 tcattagtgt tcgacggacg acagacagta atcctctggg tatctaaagt cccgccatgg
      601 ctatacatag taggactccg agtgcacgct aaagggcggc tcgcggctgt ggaaccgagt
      661 gagcggcgcg atttagacgg cagggcaata cgcaataaga ttcttatctc gctccccgat
      721 accgagtatc gagcagcgcg ccatcatttg gaatatgtcg tgctgccgca actcagtgtt
      781 ttgcacgaac ctagcgagaa gctggagttc gcgtatttcc ccaacgcggg cttgatctcg
      841 ctcgttgtgg ccatggaaaa tggcaagacg gttgaggcgg ccgtgctcgg cagagagggg
      901 gcagccggta taccatctgc ggtccactta aagcacagtc cccttcggga agtagttcag
      961 gtggccggag acggatttcg ggtaaaggtt tcggcccttc agcccatact gaaatcggct
     1021 ccggagtttc agatgacttt aagccggtac gccatcattc agggaataca ggtcgcgcaa
     1081 acagccgctt gtaaccgact tcacgatgtc gagcagcgcc tcgcgcgctg gctgctaatg
     1141 gctcaggacc gtgtcgacaa cgcgcccgta acgattaccc atgatttcct ttccaccatg
     1201 ctcggtacgg atagacctag cgtcagtctc gctgccggcg tgcttcaaag gaagagatac
     1261 atcgagtaca cccgcggcgc ggtgaaggtg ctcaatcgca agaagctaga aagttcagca
     1321 tgcgaatgtt acggggtaat tcgccaatac aatgccgaga ttggtgttcg gtagggtctt
     1381 ccgggttgcg tcgggtatca aacagacaag caaagccaca aatagcatct tgtagaactt
     1441 tggctgctaa ggctgtgggt aaatagcaga caaggaccgc cggatggacc gcatttacga
     1501 tatctttaga aagatgcccg ataattcgcc gatttgggtc gagtccgtgg atgggctgga
     1561 agcattgaag gaaaggcttt tataccttgc atccacgaaa ccgaatgagt acctcgtcta
     1621 cgaccacggt ttgcaaagat ttatcgaccc gtttgataat ccacgcgagt tcctgcaagc
     1681 gaaataacgc gcgctagcag ccggatcttt cgaggccgcg ggacgcccgg agcagttttg
     1741 gtcatccccc accacaaagt tccgctgcca ccgatcgcta ggtttacaat atttgcatcc
     1801 cgatggaagc attgaggaaa ccgtcgatca gcctttctgc tcgtcgcacc gtggatctgc
     1861 gcttttccga gttaacagat aaggtacggc gcaatcgccc gggcgacgat ctcgatctgc
     1921 tccgccgggc ctacgagttc gcggccgaac aacataaaac gcagacccgg cgttccggcg
     1981 agcccttcct gtcgcatccg cttgaggtgg cgcatctgtt ggccgatatc aagctcgatg
     2041 tgacttcgct gtgtgccgct ctgctgcacg atgtggtgga agacaccaag attcccgtcg
     2101 aaaaaattgc cgagcagttc ggcccagatg tagcgcgcct cgtggaaggt gtcacgaaaa
     2161 tcagcaagct cgatttagta gctccagaag accggcaggc cgaaaacgta cgcaaaatgc
     2221 ttctcgcgat ggtcaacgac gttcgcgtcg tgatggtcaa gctggccgac cgcctccata
     2281 acatgcgcac gctggaatat ctcactcctg aaagccaaca gcggatcgct cgcgaaacgc
     2341 tcgatattta tgctcctgtc gcaaatcggc ttggcatggg cttgattcgc ggagaactcg
     2401 aggatctggc ttttcggtac atcgaaaccg acgcttttct cgaattgcag aagaaagtgg
     2461 cctcgaagca aaacgtgttt gacaagtttc tggacgaagt ccaggagacg atccgcgaaa
     2521 aaatgatcga aaacggcatt ccagccgagg tgcaggcgcg aatcaaacgg ctttactcgg
     2581 tgcacttgaa gatccaaaaa cagcagcgca atctcgacca aatctacgat ctgctggccg
     2641 tgcgcgtggt caccgatacg gtgaaaaatt gttacgctgc cctcggggta atccaccaga
     2701 tttggcgtcc ggtgccggga cgatttaagg actacatcgc gatgccgcgc cccaacctct
     2761 accagtcact gcacacggcc gtgattcatt ctggacagcc gttcgaagtg caaatccgca
     2821 cgcaggaaat gcaccgcatc gccgaacagg gcgtcgccgc gcactggaag tacaaaggcg
     2881 ggccggaggt agcatcatcg gcagacgatc aacgcatcgt ctggatgcgt cacttgatcg
     2941 agtgggtgca ggagatgcag gagcccagcg agtttctatc aacactgcgc gtagaccttt
     3001 acccggagga agtgtacaca ttcactccaa agggacgggt ggtcgtcttg ccgcgcggcg
     3061 ctacccctgt agatttcgca tatgcggtac ataccgaggt gggccaccag tgctcggggg
     3121 cgaaggtaaa cggcgagatg gttccgttgc gccatgccct caccaatggc gacgtggttg
     3181 aaatcgttac gcagaaagga cacacgccgt cgcgcgattg gctctcgttc attcacactt
     3241 cgcgagcccg cagcaaaatt cgccaatgga tcaatctcca tgaacgccag gaagccaccg
     3301 atgtggggcg gcgcttgctc gaaaaagagg cgcgccaagc aggtgtaagc ctcaagaaaa
     3361 tttccagtga ggacttacag cgcgtggcca ctgaatatgg ctgctcgcgc gtggacgatc
     3421 tttatgccga tctgggctac ggaaaatggt ccgcgcgcca ggtaatcgcc aaagctacgg
     3481 gccagccctt gcccgcaaat ttcgaggaaa aaccgcccaa gcccgcttca aatatccggc
     3541 gaatgctagg cctggatgaa gccgcgatca tcgtgcgcgg ccatgacgac ctgatggttt
     3601 atcgttccaa gtgctgcaat cccattcctg gcgacgacat cattggctac gttacacgcg
     3661 gccgcggaat cgctgtccat agcaagaact gccctaacgt tgagaacttg ctatatgagg
     3721 cggatcggcg aatccccgtc gaatgggctg gctcgacgca cgctgagttt ccggtgcaac
     3781 tgcgcatcat cactgaagac cggcccggca tgcttgcctc catcactggc atcatcagtg
     3841 aaacgggcgc caacatccga acttttgaaa gtggcggaga agatattcgc gcgcgcatcg
     3901 aagttgcgct agatgtgcgc gatcgcaagc aactggaacg gattctggcc cgcatcaagc
     3961 gcattcccgg cgtcttcgac attgagcggg tttacaatgt ctagcttcca ataacgacgc
     4021 ctattaatac ttccgcgaca ccgccgactt cagctctgca gctccgcgta caatggatac
     4081 atgggatgct gctccacgga cgcgcatgat gccgcacaga cgcgattttc cgggcttatt
     4141 ttgtgctcgc tcttgatgag cttcggtctt ctcctttcgg gatgccacat cctgcacccg
     4201 gttgatacaa agcctcttga tgctgttgga atgagttacg acgccgtcca ggaactcaaa
     4261 tccctgcgcg tcagcacggc cgaggtgacg gaaattgcca aagcacgcca gagcggattt
     4321 tccgacgcag attgcatcga cgctttcaaa gttttccgga gccgcagcca gtccttcgac
     4381 gccggtgatg ccattgcaag tttggtggag gtgagcgtta gtgaggacac gatcctagaa
     4441 ctcgccagga tcaatcagct cgggctagac tccggcgaac tccaggcgat gaagctcgcc
     4501 ggcctctcgg acgatattct cttggaagtg gctcggcacc gcgcgcacgg caagcccgtg
     4561 ctctccggcg cttcgctggc gaggctaaag aatacgggta tgcgggcatc aacgctattc
     4621 gaactcgcgc aacgaggtgt tccggattgg caggagtccg cgatcgtgtc ctatcgccgc
     4681 cacggggcca gcgacgatca agttctccgc cgttttgccg gtatctaggc ttctgcccat
     4741 caatttgttt acccaagctc cttctcagcc tgaaggaaaa acctatcgtt tttgttggta
     4801 ttttggtggg cctgggtaga cttgaactac cgacctcacc cttatcaggg gtgcgctcta
     4861 gccacctgag ctacaggccc aatgagcgtc attttatcat cagcgaatcg atgagcctag
     4921 caactcgtca cagcgatccg tgttgcgcct gagaattctc cctccccgcg ggcaacacgt
     4981 gtacaatacg ggccactgtc acctcgggat gcggggtggg tttccgcgac cagcgcggag
     5041 gtgtgcatgc gtcgggcgcg tttttctccg gtagctttga tcctgctcct tctgtgttgt
     5101 gtccgcgtga ccgcacgcca agctccgcag gaaaatcttg ctgtctcgac gaagtcaatc
     5161 gaagctccac tactcaaagt ttgcttgcgc cttgaggaca acacctcctt cggcggagtg
     5221 atgcgcgtcc gtctattgcc tgccgagggc gacaagatca gcggcgttcc caacggatcg
     5281 gatggcgaaa cgacttttgc caacgtgcag ccgggcactt atctcctgga ggcgagcgct
     5341 ccaggattcg tgactgtccg gcgagaggtg cggattgaac tgggtcatgg ccttcaaact
     5401 ctcttcgtga cgatgaaacc catgccgttc gcggcgcgtc ttccggaagc atcgccgaca
     5461 tcgagcgacg cccagccact tcccgcgtct ctcaccggag ggccctcgcc tccgcgcgcc
     5521 gcttcgcgag gggccgcgct cgtctcctgg attccaccga gcctcgatac caccgtgccg
     5581 gcggttaacc ccagcgttcc ctgccccctg cctggtctct tgaaaatagc aggcgtgcgc
     5641 atgactgaat tcgtggctaa tctggaaaga ttcaacgcca ccgaacaggt ggagcatttc
     5701 aagattaatg cagcgggctt gcgaggctcc gcggatgtgc gctcttttga ttacgtcgtt
     5761 ctcgtctcgc gaaatcgttc tggattgttc caattggaag aataccggaa cgggaccgtc
     5821 gacccggggc tattcccggc cggggtggcc acactcggat tgcccgcgat ggctttgatc
     5881 tttcatccat ttctcgtctc ggatttcgat ttcaagtgcg agggactcgg gcaattggga
     5941 ggacgtgccg cttggcaaat tcactttgcg cagcgacctg accggccaag ccggattcgc
     6001 gaatacgtca ttggcaaaaa ttcatatccc gttccaatca agggacgcgc ctggatcgat
     6061 cccggcacgg atcaggtggt gcggttggaa tctgagttgg tcaagccgat cccggagatc
     6121 gccctgacaa aagagcgttt gtccattgag tatgagccgg tggaattcca ttcgaacggg
     6181 cagcggctct ggctccccga gaccgccgaa gtttactccg aacgcggcac agtgcgctat
     6241 taccgccggc atactttcag caacttcaaa ctcttcacgg tggcagcgga ccaaagcatt
     6301 cagccgaagg aagcatatgt tttcacgaat acgtcggacc gtgacgttgc tggaatcctc
     6361 acagtgttcc ccgtcccggg atcaacgctt caaccggttt cgatcacatt cacgatcccg
     6421 gctggcaaaa ccatcttaaa atccgtggga gtcggcaagg atattcgtgt tcccccggtg
     6481 tctctagggt ccgcaacgtt ttctcacaat ggcccggaag gctccatcac cgtgaatgct
     6541 tacctgacca acgacagtag cgtagacgtc actcccaatg ccgacatctc gatgagtact
     6601 gttccgatcc aaaactagac aaggcgaata actgctcggt ctagacaatc tgtagtccgt
     6661 atagccaaac gcgaacgtcc acggcaaact agtgccttgt cgtcttctag cggaggacat
     6721 tcatggactc atcgtctccg aagatcgcca tcgtcactgg cgcaggcagc gggatcggca
     6781 agagcgtggc cgttgcgctg gcgcagaact gctactcggt ggttcttgcg ggccggcgca
     6841 aggcgccctt ggaaaccacg gcgcttgaag ttaggcaggc caattcgcaa gctctcgtcg
     6901 tttgcgccga tatcaacgat cccgccgccg tgcgtgaact tttcgcgaaa acaaaggatg
     6961 ctttcggccg tctcgatcta ctcttcaata atgccggcat ctccggcccc gccattccgc
     7021 tcgaggattt agcctacgaa aagtggaagt cgatcgttga tacgaacctc accggaactt
     7081 ttctttgcac gcaggaggct ttcaaaatca tgaagaacca ggagccgcgc ggcgggcgca
     7141 tcatcaacaa cggctcgatt tccgctcacg cgccccgtcc caattccgct ccctacaccg
     7201 catccaagca cggcgttacc ggtctaacca aagcgacgtc tctcgatggc agaaagtacg
     7261 acattgcctg cggtcagatc gacattggaa atgcttcgac gcacatggcg gaacggatga
     7321 agaagggcgt ccctcaaccg aacggcacgg tcgccgtcga gcccaccatg gatcccgccg
     7381 atgttgcccg cgccgtcgtg tacatggcct ccctccctct cgatgccaac gtccagttca
     7441 tgactgtcat ggccaccaag atgcctttca tcggccgggg atgatcctcc gtgatttgac
     7501 gatgaatttg cgattgagtt aagtccgtag cgcgcctgaa ttatcccagc aatggttttg
     7561 tgcccgctga aactgccagg ccatgcgaca acagcgccgg ccaatgcccc aacacgctca
     7621 acaccacatc agttagatgg ggcttgtcag acaaacgcaa aatcgcctgc gtcagtaaaa
     7681 catagccgcg aagcattctt tgccgctcgc gttcgaatga ccacagccaa tcatcttcgt
     7741 tgagttcgtt cgcagcgatg tagcctgcca aaagctcggc agccatcaac gcttgagtca
     7801 ttccgccgcc ggtgaccggg tcgacgaatc ccgccgcgtc gccaagcaaa actactccgg
     7861 gtgccactcc tgcgatcgcg cgtcccgaga gtggcgatgc cccgatcgtg gcactcacct
     7921 ggttcgcgcc ttccagcagc gaggccagtt ccggaaatgc ctcgcaacgc tgaagcaagc
     7981 tgattttcct gtcttcctga gtcccactaa cctcgcccag agcgatcaca tttatttcgt
     8041 ggtcggggag cggagtaacg tagatctcct ttcccttgcc gacaaatatt tctacccgat
     8101 cggactgctc tttgttttcc gacaggcgaa agtgggcacg aatcccaaat cggccgcggc
     8161 gcgggggaca gctgagcccc aacttttgcc gtatcaccga gtgcatgcca tcggccccga
     8221 ccacgagcgg cgcgagcacc cgttttcctc ccatgatcaa ccccgcaaca cgtccgttct
     8281 cgattgatgg cccttcgact cggcatccgg tgcgaatgcg aaccccgggg gttgctgcag
     8341 cctccgaata gagaacatga tccagaactt tgcgtcgctg gcctatcccc gtctggccga
     8401 acccgactcg ctttgggaaa ttgccttcca ctgttcggcc gttgaaatga tatcggacac
     8461 cccgaaagcg agttccgccc acggaatcag tcactttcaa gcgatccaaa acggccactc
     8521 ctccaggcat cagtccttcg ccgcacggct tttctctcgg gaactcagcg cgctcgaaca
     8581 actgaaccgc aaggcccctt cgtccaagca tgatggccaa tgacgaacct gctattccgc
     8641 ctccggcgat aagcacatcc gcagttcctt tgtccgcgga actcttggag cttgtgctgg
     8701 gaatgctggt cttgttgctg ttttccggga gaggatcgac aaccgaagtc gctaaaactt
     8761 ctggatatcc cagcatgtag gtcacgggaa ctatccccgc agcccctttt ccgatctgcc
     8821 acctatagcc gtcggcgctg atagaggaaa caagctgcga aagttcggtt gtcgaataag
     8881 cgcgcaggca agagaggatg ccatcgatca gtagcacgaa ggggattact ggtatcacgt
     8941 aggtccaaaa cagccgggac caccggaagg gccgcatgaa gggtgccacc agaagagccg
     9001 caaccggcgc gaggaaggtc agcagcacat tcaaggggtg gcgcgctgct gcttcaaaaa
     9061 tcccaattcc ttgccgcctg tctaccgcat tctgaagaac gccagccgct tctttcggag
     9121 aaaagtggtg gaaggaacta aagatcgttc gaaacccggc gacttgcgcc gggatcctga
     9181 ccgcgtcgac cggttcggcg aaataagtga tcgcaccacg cgattcttgc tgcgcgtttt
     9241 caaatgcggc tagatttgga tatttgtcag tcagacagac accaatcttc gcggtcgctt
     9301 gatcggcaat cgttcgatgc aaccacgtcc acgggcctcc ggcgcctgaa cacaggtcga
     9361 ccactcggtc tgctcccgca gctttgattg cttgtgcgag gcgcgaggca acagggcgat
     9421 agatgttgcc ccaatttaag attacctgga gcgcatccgt cacgtgatcg cgaagatttt
     9481 taggaaacca cgcctgctcg tgaatctcgg acacattgat gcgcttcatc gagaatagtt
     9541 ggcgtgagtg tgctgtaagg tgctgatgcc gtcaacagcc ttagagtggc ggggggcaca
     9601 aatggtttgc cgagggttag gagaatcacc tcttgcttcg cagcgggcag tcgcctgagt
     9661 gtcgcgacta ttgccggcgc gctgctggcg gattggcgag tcgcggcgtg acggtgaact
     9721 cgtggacgtg catcacggcg atgagccatg agccatcgtt ttgcttcatc ataatcaaac
     9781 tgtggatgcc cttgcgggtg ggccgcatgc ttccatcttg attcttcgcg ccggtcatct
     9841 gccagtccac gtcgactgcc gccacgtcag gcttcaaaaa ccgtatactt cgtacctggc
     9901 ccgtctgatg cgtctcgctg taaactccgg tcataaacgg tccgaaggtg gcttccattc
     9961 cagcacgtcc gtgcgttgga tccccgaaat tgttaacgaa atcgcagtct tccgtatagg
    10021 tcatcgcaac ggcgtgtaca tcatgcttat tccacatatc catatagttc tgcacggtct
    10081 gtgtgatggc ggcgcggtcc tttttctgtg aatcgttcgc cgcgcggccg tgtcgtgccg
    10141 cggtcgc
//
LOCUS       DATNDT010000080        12195 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2034455, whole genome shotgun sequence.
ACCESSION   DATNDT010000080 DATNDT010000000
VERSION     DATNDT010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2034455"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1537)
                     /gene="mtaB"
                     /locus_tag="VN788_02420"
     CDS             complement(173..1537)
                     /gene="mtaB"
                     /locus_tag="VN788_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HXU19418.1"
                     /translation="MATFSIENFGCRATEADAAGIRHELLNSGLTLASSHDSASVIVL
                     NTCTVTAAADAQAREAIRKIHAANPGACVIVTGCYAQRAPAELAAIPGVTWVVGNSHQ
                     TRIPGLVREFAGGIAHERAAGAADFISVDTLRGVISSGAAKVISGDIFEQLPVQFAPS
                     KFMDGDRTRPILKLQDGCSNRCSYCVIPFVRGRSRSLPPDQAISKIRALADAGVQEIV
                     LSGINLGSYGRDLTPRVELAGLLQRILTETSIPQLRFSSIEPQDVTEDFVSLMASSTR
                     VAPHFHIPLQSGSDRVLKAMHRWYRAAHYAERVRMIRRVLPDAAIGADVIVGFPGETD
                     KDFSETIGLIRDFSFTYLHVFSFSARPGTAGATLNDRVPPNKIRERARALRALARQKS
                     TEFRASQSGRVLRALTLARGCDAWTEAITGNYLKVRIRGRHAANSWVEVRVGAEGNVE
                     AVRA"
     gene            complement(1620..2306)
                     /locus_tag="VN788_02425"
     CDS             complement(1620..2306)
                     /locus_tag="VN788_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU19419.1"
                     /translation="MSKSNGKASLKVCLLSPHPMVLDEFHRLLEDSGFQITSKHLTSM
                     LAPDLRNLDIPRASVYVVDAHAAKQATGALLGNVLERYSGARLLVVGDKLSETESYSL
                     LRLGAKGILSYQEARDQLSRALPQVAAGGIWVPRPVLSRFVDSILSSTHGRRLRVDSP
                     NELSRREQEVLSSLLENLANKEVAERLHISERTVKFHVSNLLAKFGVRRRADLILLCY
                     QRRNTAVPQL"
     gene            complement(2405..3496)
                     /locus_tag="VN788_02430"
     CDS             complement(2405..3496)
                     /locus_tag="VN788_02430"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU19420.1"
                     /translation="MVSSAQASPEAASVELPPEQIYFGPSATMQAVRQRVGRAAGLNV
                     PILILGESGTGKEVLARFIHSRSPWSNGPFVKVNCPAIPGTLLESELFGFEKGAFTGA
                     YAAKPGRMELAEGGTLFMDEIAELDSSLQAKLLHVLQDGHFTRIGDHEERRMGARVVC
                     ATNRLLREEVANGNFRADLLYRINVITITLPSLRERREDIPYLIEYLRNQFNRRFERD
                     VAPLSQETVHLLQQRDWPGNVRELENSIARYVVLGSEETFYGERVDRPARIVYEPNAD
                     GHIPLKRISQQAIRQMEHDVILRVLQANHWNRRKAAQVLKISYRALLYKVRQAGLPAK
                     HPRQNVQTDLEVSPPAGPPIRKTAVRGTS"
     gene            complement(3827..4165)
                     /locus_tag="VN788_02435"
     CDS             complement(3827..4165)
                     /locus_tag="VN788_02435"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU19421.1"
                     /translation="MTERRMARRYDLSLPVNIRLPINLEPKSDSGQTRDISTRGVYFT
                     AHKDLAAGTELDFTLTLPSEITRGSEVFVRAHGRVVRVDKRSDDNHETIGVAAVIERY
                     DIVRGEGTRV"
     gene            complement(4584..6098)
                     /locus_tag="VN788_02440"
     CDS             complement(4584..6098)
                     /locus_tag="VN788_02440"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU19422.1"
                     /translation="MAEKQKIMVVDDEPSIRKYLRTLLEVDGFDVETLPNGKEALKEV
                     GAGSRPDFIILDVLMPEMDGLETLRQLMQMDRSLNVIMLSCANEVTTVVEAIRLGAMD
                     YLTKPFEKPELDAAFLKCQQKQQLRSENQALREYCEALTEDISFLAASPQMLKIRQQI
                     LQIAPVDVPIFISGESGVGKEVVARMVHLRSKRQHQPFIKVNCAALPGELLESELFGF
                     EQGAFTGAVRAKPGKFELANKGTIFLDEIAEMSPHLQAKLLHVLQDGQYSRLGARAVV
                     NVDVRVLAATNMDVKEAMKTGRFREDLYYRLNVLSLHIPPLRERITEIPLLFRHFLVK
                     YSEKYQKQAPDPSKSLLEAAVRYPWPGNLRELENFVKRYVILEDDEGSFRELLEMTGQ
                     QQRMPVQDEAPPPKEQGLKALVRGLKDEAEMEAIAGALEKTNWCRKDAARMLGISYKA
                     LLYKMRQFNLDSGRGSRSSAAREAAKAAKEAAAKEAAVREATLKDGPAIPAIRD"
     gene            complement(6123..7340)
                     /locus_tag="VN788_02445"
     CDS             complement(6123..7340)
                     /locus_tag="VN788_02445"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HXU19423.1"
                     /translation="MSKNMIVEKQPSGAVTFQLVASLLDALDRPLLVSDRSGQVLFAN
                     LHAQDALAVRNFNTAGELNLFRDLLRVDRKEIIGQLEAGEQEIDLPLDHSSGKFRARV
                     RWLPEPDWLAVFVEPSASEAPADEAEMRQTVQELMQEREITYRNLLAAYLRLQEANRQ
                     KTVFLASAAHELKTPLAVMKGYYELLLTGSLGKLSEKQTDILRESKESCDRLVRLVSM
                     FLNYSALESGKLVLQLRENDLGDCIRDLAGRWQDAFQRSGVRLEVRIGPGLPPFKFDY
                     QKVQQCIVNLLDNALKHSSAGGAVTLAAELHFWERRMGEGAAPHERRRDRGQHPNSAL
                     ISIADTGKGIAAEFHQEIFEEFVRVDPSSSGMGLGLAITKRLIQAHRGKVWVDSEVGR
                     GSSFSFLLPIRME"
     gene            7663..8004
                     /locus_tag="VN788_02450"
     CDS             7663..8004
                     /locus_tag="VN788_02450"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU19424.1"
                     /translation="MRPSADLASLTEEHRRSPRILLQVPLFVRGEDPQGEQFIELGKT
                     LDISSLGAFIASPRALQVNGFITLTIPAPSITSSGLVPAGTAPIQAKVKRQQKAGDVY
                     LAGVEFTKPLG"
     gene            8235..9422
                     /locus_tag="VN788_02455"
     CDS             8235..9422
                     /locus_tag="VN788_02455"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXU19425.1"
                     /translation="MSQPGDAFQPRPAAEDSIFELLIETLEGLDDSARAQFLKQLFKT
                     LTRLDLTETQSSEYWSRILQRRAEFTTLGKRISWKAALLDVLFSEGQLRVPVLVEYDD
                     FKRLQVDAATDSLTGLYNRRLFDEYLNKELIRAKRYSEQLAVVILDLHKLKEVNDRHG
                     HMRGDDVLKIAAATLRTTLRASDFAFRIGGDEFALLLPRTDSEQAVTLCRRVRSQYEV
                     ELLPLNLNIGVTLDFGAAVYPQDGDQRSELLGTADARLYALKASNRPSQRVIPMEPET
                     PPTAGAAPSRFPGPSVSPVKPSAVPSAEARSEHRKWERVSLAGTKAYALLSEAEQKTA
                     KVIDLSYGGVALQLDKPEDLPNQFSAILHVPILPPVRVMLRKAYMLRSVGERSRIGCI
                     FVS"
     gene            9647..10306
                     /locus_tag="VN788_02460"
     CDS             9647..10306
                     /locus_tag="VN788_02460"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXU19426.1"
                     /translation="MSLRINDEAPNFTAETSQGTISFHEWIGNGWAVLFSHPKDFTPV
                     CTTELGYMAGLQPEFEKRNCKIIGLSVDPVASHGKWAVDIEETQGHKVKYPMIGDPEL
                     KIAKLYDMLPADAGNTSEGRTAATNATVRTVFVIGPDKKIKLMLSYPMSTGRNFDEVL
                     RVVDSMQLTAKHQVSTPVNWKPGDDVIISGNVSDDEAKKKYPGGWKAPRPYMRIVPQP
                     R"
     gene            10333..11724
                     /locus_tag="VN788_02465"
     CDS             10333..11724
                     /locus_tag="VN788_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXU19427.1"
                     /translation="MIFKQFYLSCLAHASYIVGDESTGTAAVVDPQRDADQYIAFAAE
                     HALKIRHVFLTHLHADFVAGHLELRDRVGAKIYLGAAAKAAYAFTPLRDGDIVEFGCV
                     LLKALETPGHTPESISLLVYDLSVSDTQPHAVLTGDTLFIGDVGRPDLRVALGWSATE
                     LGGMLFDSLHAKLLALPDQSLVYPAHGAGSLCGKALSKETVSTLGEQRRSNYALQPMT
                     KEAFIKVVTADQPDAPSYFTYDAVLNSQERPTLDQTLERVNAMTLDDVLALQAVGAQI
                     LDTRDPGEFAAAHLAGSINVGLGGQFATWAGTVLDRDHPIVIIADPGRENESTTRLGR
                     IGFDHIVGYLQDGLHSLRSRPDLTVVTERVSAPFAAELLSSIQPPLAIDVRGPGERKQ
                     KHIAGSEAFPLNHLVENLHKLPRNRSLLVYCAGGYRSSIAASLLQRGGFASVSEIAGG
                     IAAWETAQLPLQA"
     gene            11914..>12195
                     /locus_tag="VN788_02470"
     CDS             11914..>12195
                     /locus_tag="VN788_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19428.1"
                     /translation="MAVQILLVDDNEVVRGRISETLKAHEGWEVCAEVANGEQAVMKA
                     RELNPDLIILDLAMPVMDGLRATREIVKILPSVPIVIYTLHYADWVELEA"
BASE COUNT         2623 a         3599 c         3376 g         2597 t
ORIGIN      
        1 ctggcgcgat atcgaggtca cccgcgaagt gacagggaag cctaccttgc gtctggccgg
       61 cgccgcgctt caaattgcgg aacgcatggg cgtcaagaat atttcggtgt ccatcacgca
      121 cagcggaaat ctggcgctcg cgcaagtgat tttcgaaaac taagcggcag agctaggccc
      181 gaaccgcttc cacattgcct tcggctccga cccgcacctc gacccaactg ttcgctgcgt
      241 gccgtccgcg aatgcgcact ttcaaataat ttccagtaat tgcttccgtc caggcatcgc
      301 agccgcgagc cagagtgagg gcacgcagta cgcggcccga ttgcgaagcc cgaaattcgg
      361 tggacttttg ccgcgccagg gccctcagcg cgcgggctcg ctcgcggatt ttattcggag
      421 gcaccctgtc gttgagggtg gcgccggcag ttccgggacg cgccgaaaac gagaagacgt
      481 gcaaataagt aaacgaaaaa tctctgatca acccaatcgt ctcgctgaag tctttatccg
      541 tctcgcctgg aaatcccaca atcacatctg cgccaatggc cgcatccggc agcacccggc
      601 gtatcatgcg cactcgctcg gcataatgcg cggcgcggta ccaacgatgc atggccttca
      661 gaactcggtc ggagcccgat tggagtggta tgtgaaaatg cggtgccacc cgggtggagg
      721 aagccatcag agacacgaaa tcttcggtaa cgtcctgcgg ctcgatggaa ctgaaccgaa
      781 gctgcgggat tgaggtttcg gtgagtatcc gttgcagcag accagccagc tcaacgcgcg
      841 gagtgagatc acgtccatag ctcccgaggt tgattccgct tagaacgatt tcctgcacgc
      901 ccgcatcagc cagcgcgcgg attttagaaa tcgcctgatc aggcgggaga ctgcggctgc
      961 gcccgcggac aaatggaatc acgcagtagg agcaacgatt gctgcagcca tcctgcaact
     1021 tcaaaatggg ccgcgtacga tccccgtcca tgaatttcga cggggcgaac tgcaccggca
     1081 attgctcgaa aatgtcgccc gaaataactt tcgccgcacc cgatgaaatg acacctctaa
     1141 gcgtgtcaac tgaaatgaag tcggccgcgc cggccgcgcg ctcgtgtgca atgccccccg
     1201 cgaattcccg gacgagaccc ggtatccgcg tttggtgaga atttccgacc acccatgtga
     1261 cgcccggaat cgcagcgagt tcggcgggcg cgcgttgtgc atagcagccc gtaacgatga
     1321 cgcacgcccc cgggttggcg gcgtgaattt tacgaatagc ttcccgcgct tgtgcatctg
     1381 cggctgccgt aaccgtacat gtgttcagca cgatgacgct ggcggaatca tggctcgacg
     1441 ccagggtcag cccagaattc aacagctcgt ggcggatacc ggctgcgtcg gcttccgtcg
     1501 cccggcagcc aaagttttca atcgagaatg tggccatgct gtgtgcagct tcttggtgtg
     1561 acgaagggaa attataacag ccactcgagc tttcaacgat gcgcgcagcg ttggagtgac
     1621 tacagctgcg gaaccgccgt gttccggcgc tggtaacaga ggaggatcag atccgcgcgg
     1681 cggcggacgc cgaacttggc gagcaggttc gatacgtgaa atttcacggt acgttcggaa
     1741 atgtgcaggc gctccgccac ttctttgttg gcgaggttct cgagcaagct gctgagcacc
     1801 tcttgctcgc ggcggctcag ttcattggga gagtccacgc gcaagcgccg tccatgcgtg
     1861 ctgctcagaa tggagtccac gaaacgggaa agaactgggc gcgggaccca aatcccccca
     1921 gcggctacct gcggaagtgc gcgcgagagc tggtcccgag cttcttggta actcagtatc
     1981 cctttcgctc ctaggcgcag caaggaatag ctttcggttt cactcagctt gtctcccacc
     2041 accagcaaac gcgcgccgga atagcgttcc aggacgttgc cgagcagtgc cccagtcgcc
     2101 tgtttcgcgg catgggcgtc cacaacataa acggaggcgc gaggaatgtc caaattgcgc
     2161 aagtcgggtg ccagcatcga ggtcagatgc ttggacgtga tctgaaagcc cgaatcctcc
     2221 agcagtcgat ggaattcgtc caacaccatc gggtgcggcg agagcaggca gactttcaga
     2281 ctcgctttgc cattggattt cgacatgttt ccaatcaggc tagccgaatt accaggaagg
     2341 ggcaaggcgg agtcaagatc gaatttcata ccaggcacgg ggtcgtaccg gtgtaggcaa
     2401 ggaactagga tgtgcctcga accgccgttt tccgtatcgg tggcccagca ggcggcgata
     2461 cttccagatc ggtttgtaca ttttgccgag gatgcttcgc cggcaaacct gcctggcgga
     2521 ctttgtagag cagcgcgcga tagctgattt tcagcacctg ggctgccttg cggcgattcc
     2581 agtggttggc ttgcaggacg cgaagaatca catcgtgctc catctgccga atcgcctgct
     2641 gagagatgcg ctttaaggga atatggccat cggcgttagg ttcgtagacg atgcgcgctg
     2701 gccgatcgac gcgctcgccg tagaaagttt cctcggaacc gaggacgacg taccgggcaa
     2761 tcgaattttc cagctctcga acattcccag gccagtctct ttgctgcaga agatgcaccg
     2821 tctcctgcga cagcggggcg acgtctcgtt caaagcgccg attaaattga tttcgcagat
     2881 actcgataag ataagggata tcctcacggc gctcgcgcag ggaaggaagg gtgatcgtga
     2941 ttacgttgat gcgatacagc agatccgcgc gaaagtttcc attcgcgact tcctctcgaa
     3001 gcaaacggtt ggtggcgcag actacccgcg cgcccattcg cctttcctca tggtccccta
     3061 tgcgagtgaa atggccgtct tgtagaacgt gcagcagttt ggcctgaaga ctcgaatcga
     3121 gttctgcaat ctcatccatg aagagcgtgc cgccctcggc caattccatg cggccgggct
     3181 tcgcagcgta ggctccggtg aaggcgcctt tctcgaagcc gaacaattcg ctttccaaca
     3241 gtgtcccggg aatagccggg caattgactt ttacgaaggg cccgttgctc cagggcgagc
     3301 ggctatggat gaagcgcgca aggacttctt tgcccgtacc gctttcgccg aggatcagga
     3361 tgggaacgtt taggccggca gcgcgcccca cccgttgccg cacggcctgc atggtggcgg
     3421 atggtccgaa gtaaatctgc tccggcggca actcgacgga ggcggcttca ggactagctt
     3481 gagcggaaga aaccattcgg tgcgtagtgg aagaacctcc cccgcaagtg tctagcacac
     3541 ctgcaaccaa accagaatcg gcgaagatcg ctggagccgc ttcgaactcc agccttctgg
     3601 ccctgcaaac ccttagtttc tttctctttg gaagatcgtg actgcaggca gacctgcggt
     3661 ggaggacggc cggtgaaccg gagaacatgc aagttgcctg aaaacggttg gcacttaggg
     3721 gaagcgcggt tcctcaccag ggcaccggct ttcctcgccg tagccagaca aagttagtac
     3781 gcgctcttgg tgctcaggaa gttcgttgga tagccggcca atctacctaa acgcgagtgc
     3841 cctcgccacg aacgatgtcg tagcgttcaa ttaccgccgc caccccaatc gtctcgtggt
     3901 tgtcgtcgga gcgtttatcc acgcgcacca cgcgcccgtg cgcgcgtacg aaaacttccg
     3961 aaccgcgggt gatttcggag ggaagagtga gcgtgaaatc gagttcagtg ccagcagcga
     4021 gatccttatg cgcggtgaag tagacaccac gtgtggagat gtcacgggtc tggccactgt
     4081 ccgatttagg ctcgagatta atgggcaaac gtatattgac cggaagtgat agatcgtagc
     4141 ggcgcgccat gcgacgttcc gtcatccctt atgctccttc aggattggat tgaagtgcac
     4201 agcggtaccc ggatgggcag caatcgctgt tccaaacctc gcgtcgcctt cacgatagcc
     4261 cgtgatcctc ggagacgact tgaaatgaaa cggtgcaaaa gaaactctcc acgaataacg
     4321 aacacctgac ctgggctcac acagttcgca aggcgcgcga ggacgctcaa agcgaaacgt
     4381 atttttcgga cgagaatata aactaagcag cggagaaaag gatagcctat ttcgctacgt
     4441 gcgtgccaga tgggtacccc cggcgggata taggactgct caaactgccc agcaccgtgt
     4501 gaaggaaaaa atcgcccagg ctgtactctc agaagcgctt ggttgccaga ttttggatct
     4561 tctaaccgaa acatctctcg gaatcaatcg cggatggctg gaattgccgg tccatccttt
     4621 agcgtagcct ccctcaccgc cgcctctttc gcagcagctt ccttcgctgc cttagctgct
     4681 tccctcgctg cggaggatcg cgagcctcgg cccgagtcaa gattaaattg gcgcatttta
     4741 tacagcagtg ccttgtagct gattcccagc atgcgcgccg cgtccttacg gcaccaattc
     4801 gttttctcca gggcgccggc gattgcttcc atctccgctt cgtcttttaa gcctcgaacc
     4861 agcgctttga gcccctgttc ctttggaggg ggagcctcgt cctgcaccgg cattcgctgc
     4921 tgttgccccg tcatctcgag gagttcgcgg aaactgccct cgtcgtcttc gagaatcacg
     4981 taacgcttca cgaaattttc cagttcgcgg aggtttcccg gccacgggta tcgcacggcc
     5041 gcctccagca agcttttgga gggatcgggc gcctgcttct gatatttctc gctgtactta
     5101 acgaggaaat gacggaacaa cagcgggatt tccgtgatcc gttcccggag cggcgggata
     5161 tgaagagaga gcacattcaa ccgatagtaa aggtcctctc ggaagcggcc cgttttcata
     5221 gcctccttca cgtccatatt ggtagctgcc agaacgcgaa cgtccacatt caccaccgcg
     5281 cgggctccca ggcgcgaata ttggccgtcc tgcaaaacat ggagcaactt ggcttggagg
     5341 tgcgggctca tttcggctat ctcatcgaga aaaatagtgc ccttgttcgc caattcaaac
     5401 ttccccggtt tggcgcgtac tgcgcccgtg aatgcgcctt gctcgaagcc gaaaagttcg
     5461 gattccagca attcccccgg cagcgccgcg cagttgacct tgataaacgg ctgatgctgg
     5521 cgtttcgagc gaaggtggac catccgggcc acaacttcct tccccacgcc gctctcgccg
     5581 ctgataaaaa tgggcacgtc cacgggagcg atctgtaaaa tctgctggcg tattttgagc
     5641 atttgcgggc tggcggccag aaaggagata tcctcggtca gagcctcgca gtattcgcgc
     5701 agcgcttgat tttcggagcg aagctgctgc ttttgctggc acttcaggaa ggcggcatcg
     5761 agttcgggtt tttcaaacgg cttggtaagg taatccatcg cgccgaggcg gatggcttcg
     5821 accaccgttg tgacttcgtt ggcacaggaa agcatgatga cgttcagcga ccggtccatc
     5881 tgcatcaact gccgcagagt ctctaggccg tccatttccg gcatcagcac gtcgagaatg
     5941 atgaagtcgg gacgcgatcc ggccccaacc tctttcagag cttctttgcc gttgggcaaa
     6001 gtctccacat caaacccatc gacttcgagc agcgttcgaa gatatttccg aatgctcggc
     6061 tcgtcatcca ccaccataat tttttgtttc tcggccatga tcgtccctcg aaaatctttc
     6121 ccttattcca tacgaatggg taatagaaat gaaaaggagc ttccgcggcc gacctcgctg
     6181 tcgacccaca cttttccccg atgcgcctgt atcagtcgct tggtgatcgc caagcctaat
     6241 cccatccccg acgacgacgg atcgactcgc acgaattcct cgaaaatctc ctggtgaaac
     6301 tcggccgcaa tccccttgcc ggtgtcggcg atggagatca gtgcgctgtt cgggtgctgg
     6361 cctcggtcgc gacgcctctc gtgcggcgcg gctccctcgc ccattcgccg ctcccaaaag
     6421 tgcaactctg cggctaaagt aaccgcccct cccgccgacg aatgtttcag cgcgttatcg
     6481 agcagattca cgatgcactg ctgcactttc tggtagtcaa acttaaatgg cggcaatcca
     6541 gggccgatcc tcacttccaa gcgtaccccg ctgcgctgaa atgcgtcctg ccagcggccc
     6601 gcgagatcgc ggatgcagtc tcccaggtcg ttctctcgga gctgcagcac aagtttaccg
     6661 ctctccagcg cggaataatt aagaaacatc gaaaccagac gcaccagacg atcgcagctc
     6721 tcttttgact cccgcaaaat gtctgtctgc ttctcgctta gtttcccgag agagccggta
     6781 agcaacagtt cgtaatatcc cttcatgacg gcgagcggcg tttttagttc gtgagcggcc
     6841 gacgccagaa aaacagtttt ctggcgattc gcttcttgaa ggcgaagata cgccgccagc
     6901 agattgcggt aggtaatttc ccgctcttgc atcaactctt gtacagtctg tcgcatttcc
     6961 gcttcgtcag ccggtgcttc cgatgctgat ggctcgacaa acaccgccag ccaatcgggc
     7021 tccggtagcc agcgaacccg tgcgcggaat ttccccgagg agtgatccag tggaagatcg
     7081 atttcctgct ccccagcttc cagttggccg ataatttcct ttcgatcgac gcgcagcaaa
     7141 tcgcggaaaa gattgagctc tccggctgtg ttgaagttgc ggacggcgag ggcgtcctgg
     7201 gcatgcaggt tcgcaaagag gacttggcca ctgcgatccg ataccagaag gggacggtcc
     7261 aaggcgtcca gtaggctcgc aaccaattgg aacgtcacgg ccccgctcgg ctgcttttcc
     7321 acaatcatgt tcttggacaa ctttcgctcc gttggtatcc gattaaagcg gaagacaccc
     7381 ctctgtttag ggccgaaaat ggggtcgacc cgtatgctcg caaagggatt agggggtgtc
     7441 aatggtaaat gcgtcccact ttgcacaatc gtggaaatga attgccgcaa attagccctt
     7501 tctcgcgttc ttaaatctct cgatgacgcc cgataaactt tgtaagatac tgataaataa
     7561 cggtttgcaa aacaacatgt ccagtgcgtc ctaacttgca actgaaaaag ggcgtccgcc
     7621 cgagtactaa tactggcgtt tatgaccgac agggaaactc ttgtgagacc gagtgctgat
     7681 cttgcctcgt tgacggagga gcatcgccgt tcgccgcgaa tcctcttgca agtgccgctt
     7741 ttcgtccgcg gagaagaccc acaaggtgag cagttcatcg aactcggtaa gaccctcgat
     7801 ataagcagtt taggagcttt catcgcaagc cctcgagcgc ttcaggtgaa tggcttcatc
     7861 accctcacca tcccggctcc ttcaataacc tcgtcgggtc tggtgccggc aggcacggca
     7921 cccattcagg ccaaggtaaa gcggcagcag aaagccggtg acgtgtactt ggccggcgtt
     7981 gaattcacga agccgcttgg ctagctgcct cgtcgctggc cacaattttc gacgtgccgt
     8041 ttgcaagagt ggaacgggtt ttcacctttc gcgctgcctc tcacttcggc gcgctagtca
     8101 ccataagact tacaaccctc tgcgggcctc caagttgtac ccagaatggc ctaaccattg
     8161 ctagaatcta tgtgcccccg taatctgccc accttatgcg aggtaggctg agagtagttc
     8221 ggaattaacc gttcatgtcc caacctggcg acgctttcca gccgcggcca gcggctgagg
     8281 attctatctt cgagctgttg atcgagaccc ttgaaggcct cgatgactcc gcacgtgcac
     8341 aatttctgaa acagcttttc aagactctta caagactcga tttgaccgag actcagagca
     8401 gcgaatactg gtctcgaatt ctccagcgcc gcgccgagtt caccactctc ggaaagagaa
     8461 tctcctggaa ggcggcgctg ttggacgtgc ttttttctga gggccagctg cgcgttcccg
     8521 tccttgtcga gtatgacgat ttcaaaaggc tgcaggtcga tgccgcaacc gattctttga
     8581 cgggcctcta taacaggcgg ctcttcgatg aatatttaaa taaggaatta attcgcgcca
     8641 agcgctacag cgagcagtta gcggtcgtga tcctcgatct gcacaagctc aaagaggtca
     8701 atgatcgtca cggccacatg cgaggcgatg acgtgctgaa aattgccgcc gccacgctgc
     8761 gcaccacgtt gcgcgcttct gacttcgcct tccggatcgg cggagatgaa ttcgcattgc
     8821 ttttgccgcg gacggattcg gagcaggcag tcaccctatg ccgtcgcgtg cgctcgcaat
     8881 acgaagtcga actccttccc ctgaatttga atattggagt gacactggat ttcggcgctg
     8941 cggtctatcc acaggatggc gatcagagga gcgaactgct cggcaccgcg gacgcgaggc
     9001 tatacgcttt gaaagcctct aatcggccct cccagcgggt aatcccgatg gagcctgaga
     9061 cgcctccgac ggctggcgct gcgccgtcca gattcccggg tccctctgtt tcgccggtaa
     9121 agccgagtgc tgtgccttct gccgaggcgc gcagcgagca ccgcaaatgg gaacgcgtat
     9181 cgcttgccgg aaccaaggct tacgccctgc tcagcgaagc agaacagaaa acagccaagg
     9241 ttatcgattt gagctatggc ggcgtggcac tgcaactcga taagcctgag gatttgccca
     9301 accagtttag cgccatactt cacgtgccaa ttttgccccc cgttcgcgtg atgctgcgca
     9361 aggcctacat gctccgaagc gtcggcgagc gctcgcggat cggctgcatc ttcgtctcct
     9421 aagcacacca tcgaaaatcc acgccgcccg gctgaagctg gatcatcgcc ggggacgatc
     9481 cagcatcaca tctgatcgct ccggcactcg ctcggctacc tcttctcaaa ttacacaaat
     9541 ttggtttccg cgccgcgtaa actttgctaa gctcccacgc actggcgggg aaatcgagtc
     9601 acccaagcca taagtgaaat gaagccgaag ataaaaggag aaatacgtgt ccttgcgcat
     9661 aaatgacgaa gcgccgaatt tcacagccga aacttcccaa ggcacgatca gcttccacga
     9721 atggatcggc aacggctggg ccgtcttgtt ttcgcatccg aaagatttca ctcccgtttg
     9781 caccaccgaa cttggctaca tggccgggct tcaacccgaa ttcgaaaagc gcaattgcaa
     9841 aattatcggc ctgagcgtgg acccggtcgc gagccacggc aaatgggccg tcgatatcga
     9901 agaaacgcag ggccacaaag tgaaataccc gatgatcggc gatccagaac taaaaatcgc
     9961 caagctttat gacatgctgc cggcagacgc aggcaatact tcagagggcc gcaccgctgc
    10021 gacgaatgcc accgtgcgca ccgtgttcgt gatcggcccc gacaaaaaga tcaagctgat
    10081 gctcagctac ccgatgagca ccggccgcaa tttcgacgaa gttctccgag tcgtcgattc
    10141 catgcaactc accgccaagc accaagtgtc aacgccagtg aactggaagc ccggcgacga
    10201 cgtgatcatc agcggtaacg tttcagatga cgaggccaaa aagaagtatc ccggcggctg
    10261 gaaggcgcct cggccctata tgcggatcgt tccccagccg cgttagcagt cgcgaagcca
    10321 aggtgccgca ccgtgatctt caaacaattc tatttgtcgt gcttggcgca cgcttcctac
    10381 atcgtcggag acgaatcgac aggcacggcc gccgtcgttg atccgcaacg cgacgcggat
    10441 caatacattg ctttcgccgc cgaacacgct ctcaagatca ggcacgtttt cctcacccat
    10501 cttcacgccg attttgtggc tgggcatttg gagctgcgcg atcgagtggg cgcaaaaatt
    10561 tatttaggcg ccgcggcgaa agcggcatac gcgtttactc ctcttcgcga tggcgatatc
    10621 gtcgaattcg ggtgcgtcct cctgaaggcg ctggaaacgc cgggccacac tccggagtcg
    10681 atctccctcc ttgtttacga cctgagcgtc agcgatacgc agccccacgc tgtgctcact
    10741 ggagacactt tgttcatcgg tgatgtaggc cgtcccgatc tgcgcgtggc actcggttgg
    10801 tccgccaccg aacttggcgg aatgcttttc gattcgctac acgccaaatt gctggctctg
    10861 ccagaccaaa gcctggtgta tccggctcac ggcgcgggct cgctgtgtgg caaagcgctc
    10921 agcaaggaaa ccgtatcgac actgggggaa cagcgccgct cgaattacgc gttgcagccc
    10981 atgaccaagg aagccttcat taaagtggtc accgccgatc agccggacgc tccttcctac
    11041 ttcacttacg acgccgtgct gaatagccag gagcggccta cgctcgatca aacgctcgag
    11101 cgcgtaaacg cgatgacgct cgacgacgtg cttgccctgc aagccgtcgg cgcgcagatc
    11161 cttgataccc gcgatcccgg agaatttgcg gccgcgcacc ttgcgggcag catcaatgtc
    11221 ggacttggcg gccagttcgc aacctgggcc ggtacggtgc tggaccgcga tcatccaatc
    11281 gtaatcatcg ccgatcccgg acgcgaaaat gaatcgacaa ctcgcctcgg gcgaatcggc
    11341 ttcgatcaca tcgtcggata tttgcaagat ggattgcaca gtttaagatc gcgccccgat
    11401 ctcacggttg tcactgagcg cgtcagcgcc cccttcgccg ccgagctttt gtcgtcgatc
    11461 cagccgccgc tggccatcga cgtacgcggt cctggcgagc gcaaacagaa acacatcgct
    11521 ggaagcgagg cattcccgct caatcatctg gttgagaatc tgcacaaact tccgagaaac
    11581 cgttcgctgc tcgtctattg tgccggcggt tatcgctcgt ccatcgccgc cagcttattg
    11641 cagcgcggcg gattcgccag cgtcagcgaa atcgccggcg gcatcgccgc ctgggaaacc
    11701 gcacaactcc ctctccaagc ctgaccttcc gccctttctt ctcggcctgg gattcccgta
    11761 tccatggtca acgacggcgc tggataccag gaatcctgta gaggattctg gaccatcttc
    11821 ttacggtctc cgtggtattc cccaacactc ccgcctactg tagggtaccc gacgttggtc
    11881 cgggacgctc ctgtcccggg gggagcgctc ttcgtggcgg tgcaaatact tcttgtcgat
    11941 gacaacgaag tggtcagagg gcgcatcagc gaaacgctga aggcccacga agggtgggaa
    12001 gtatgcgcgg aagtcgcgaa tggtgaacaa gcggtgatga aggcaaggga attaaatccc
    12061 gacctgatca ttctcgattt ggcgatgcct gtgatggacg gcctaagggc aactcgcgag
    12121 attgtcaaaa tcctaccgtc agttccgatc gtaatttaca ctctgcatta cgcggactgg
    12181 gttgagttgg aagct
//
LOCUS       DATNDT010000081         4580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3499228, whole genome shotgun sequence.
ACCESSION   DATNDT010000081 DATNDT010000000
VERSION     DATNDT010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4580
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3499228"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1014)
                     /locus_tag="VN788_02475"
     CDS             complement(<1..1014)
                     /locus_tag="VN788_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015225514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase"
                     /protein_id="HXU19429.1"
                     /translation="MNQPLSPELLHRMDAWWRAANYLSVGQIYLKDNPLLREPLRLDQ
                     VKPRLLGHWGTTPGLNFIYVHLNRLITSHDLDMMYVIGPGHGGPGIVASTYLEGSYSE
                     IYPNIGHDVDGLQRLFKQFSWPYGIPSHVAPETPGSIHEGGELGYSLIHAYGAAFDNP
                     NLIVVCVVGDGEAETGPCATSWHSNKFLNPATDGVVLPILHLNGFKIANPTVLARISR
                     EELIKLFEGYGYHPYVVEGHEPEAMHQLMAGTLDQILAEIQGIKAEAQAKGPDHRPRW
                     PMIIMISPKGWTGPAEVDGKKTEGSWRSHQVPFAELAEKPEHLKLLESWMKSYRPEEL
                     FDDS"
     gene            1102..1176
                     /locus_tag="VN788_02480"
     tRNA            1102..1176
                     /locus_tag="VN788_02480"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1134..1136,aa:Gly,seq:tcc)
     gene            1600..2172
                     /locus_tag="VN788_02485"
     CDS             1600..2172
                     /locus_tag="VN788_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19430.1"
                     /translation="MDIEDAQREVRSVHVGGFWGQLISSVIWLASAALGTWVTPKASI
                     LTAIVGGFFIFPLTQTALRLSGRRAQVGRENPLHWLGMQVAFVLPFSMLLLVPVGLYR
                     LNWFFPALMILLGAHYLPFASLYGMRMFLFLAGILIAMGVMIAFWFSGTLSLGAWATG
                     LVLLVFAWIGRSIATDETRTRVTTGIERTI"
     gene            complement(2273..>4580)
                     /locus_tag="VN788_02490"
     CDS             complement(2273..>4580)
                     /locus_tag="VN788_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HXU19431.1"
                     /translation="LPNGAGKAETVRICSQCHSLEQATSLRQGQAAWTDTISKMINLG
                     AQGSPDDFNRIVSYLVKFYGPIPGPSGINAPSPIKGQPTNHTATTGAASPNVESAFPP
                     VRAANRKEASLPPGGLVVDAAKEWRTYGHDPGGMRFSPLDQITPENVSRLKVAWVYHM
                     RPPGFSSAGGGGSAAPKMQGRGGAVGDEPRTPGGGRFAFGSRFRPSEDTPLVIHGLMY
                     VATPYSRVAAIDPVNGKEIWTYRLPSSNPSTRGLEYWAGDGHTPAQIVFGSRDGKLYS
                     LDAKTGKPNSAFGDNGVINLNTEAIMHGLPGRNALSSPPIVYKNLVITGGTSQENPPH
                     GPAGDVRAWDMRTGKLVWTFHSIPQPGEKYHDTWADDSWKNRAGVNVWGFLTVDEKRG
                     IVYMPFGAPSVDQYGGDRAGDNLFSSSLVAADANTGKYLWHFQVVHHDIWDADLTGAP
                     ALIDVKQGGKTIPAVVAMDKIGMVFLLNRVTGKPIYGVEERPVPPSEVPLERPSKTQP
                     FPLKPPPLVRMTFSKTDIATITPELESACKQLVQGMEVGGPFLPVSYNHLRVQFPGNH
                     GGVNWGGTSFDPALGYLFANVNQLGQISGLKDHDPKNGPAMANGQGNRVDPDGPYEGI
                     PGGGRFSVKGPGTQQLPCQQPPWGELAAVNVNTGEIAWRVPLGVTDSLPEDKRNTGRP
                     GNGGTIATAGGLVFVGATDDARFRAFDGRTGKELWTVKLKGAAEATPITYEGRDGRQY
                     VVITSTGGGFFGNPVNDDSILAFALDSK"
BASE COUNT          878 a         1291 c         1343 g         1068 t
ORIGIN      
        1 cgaatcgtcg aacaactcct cgggccggta gctcttcatc cagctttcga gcagtttcaa
       61 gtgctccggc ttttcggcaa gctcggcgaa cggcacttgg tgggagcgcc aggaaccctc
      121 ggtcttttta ccgtccactt cggcgggtcc agtccatcct ttcggcgaga tcataataat
      181 cattggccat cgcggacgat gatcggggcc tttcgcttgc gcttccgcct tgatgccctg
      241 gatctccgcg agaatttgat cgagcgtgcc cgccattagc tggtgcatcg cttcgggttc
      301 atggccttcg acaacgtagg gatggtagcc gtacccttca aaaagcttga tcaattcttc
      361 gcggctgatg cgggcgagca cggttggatt ggcgatcttg aagccgttca ggtgcaggat
      421 cggaagcacg acgccgtcgg tcgccggatt caggaattta ttcgagtgcc agcttgtggc
      481 gcatggcccg gtctcggctt cgccatcgcc cacgacgcaa acgacaatca ggttgggatt
      541 gtcaaacgcg gcgccatatg cgtgtatcag gctgtagcca agctcgccgc cttcgtgaat
      601 ggagccgggc gtttccggcg cgacgtggct cgggattcca tagggccagg agaattgctt
      661 aaagaggcgc tgtagcccgt ctacatcgtg accaatgttc ggatagatct cgctgtaaga
      721 gccttcgagg tacgtgctcg cgactatgcc cggccctcca tgccccggcc cgatgacgta
      781 catcatgtcg aggtcatgac tggtgatcaa tcggtttaga tgaacgtaga taaaattcaa
      841 tcccggcgtt gtgccccagt gcccgagcag gcgcggcttg acctgatcga ggcgcagagg
      901 ctcgcgcagc agcgggttgt ccttcaaata aatctggccg acggaaagat agttcgcggc
      961 gcgccaccag gcgtccattc tgtgaagcag ttcgggagag agcggctggt tcataatatc
     1021 ttgagcttag cgaacgactt ggacgcctgg aatttgaccg tcgcgagcgc gcttccgtac
     1081 aatgaggtca tcggacaatc agcgggagta actcaatggt agagtcacag ccttccaagc
     1141 tgttggttgc gggttcgatt cccgtctccc gctccaggac ctcaataact tacggcgatt
     1201 ttgagccgtg gccacattcc gttacattcc aataaacgtt tcactgaatt aaaccctcca
     1261 gcttttgcac ccgtggaatg caatattggt ttcgaggaat catcgatatg agcgctctgc
     1321 ccgtggaatt gccgcgcgag acgatcgtcg ttcttcggtt cgattctgac gccccgattc
     1381 cgccaagcaa gtgacattga tgtagcggta acagatgctc acgttccatc accagacgca
     1441 gaggacgacg tctggcctta tcccagatgt cgtccttaga tgtccaacca aaacttcgcg
     1501 agtgggtcag tttgaattct ctgttgatgg caccccttca aggatgcgcg gtttgttaac
     1561 gtaaagggcg gcgacacatc acaagcatcg gagatttgaa tggacattga ggacgctcaa
     1621 cgcgaagtgc ggtctgtcca tgtgggcggc ttctggggac agcttatctc gtccgtaatt
     1681 tggttggcgt ccgcggcgtt aggcacgtgg gtaaccccaa aagcatctat tctgactgcc
     1741 atcgtcggag gatttttcat ctttccgctc acgcaaacgg cgctacgttt gtcgggtcgc
     1801 cgcgctcagg tgggcagaga aaacccgctg cattggctcg ggatgcaagt cgcgtttgtg
     1861 ctgccgtttt ccatgctgct tcttgtgccc gtaggcctct accgcttgaa ctggtttttc
     1921 ccggctctga tgatcctcct cggcgcacac tatttgccgt ttgcttctct ctacgggatg
     1981 cgcatgttcc tgttcctcgc ggggattctg atcgctatgg gtgtcatgat tgcattttgg
     2041 ttttcgggaa cgttaagtct aggggcatgg gccaccggtc tggtcctact cgttttcgcc
     2101 tggattgggc gctctatcgc gaccgacgaa acaagaacac gggtgactac cggcatcgaa
     2161 cgcacgatat gaataccgca aactgagtgg cggaaggaaa atcaaactga cccactacca
     2221 acttctcgct cgtctgggca tctgaggacg tctgagctca ttcctcgatg tgctatttcg
     2281 agtcgagcgc gaatgccagg atgctgtcgt cgttcacggg attgccgaag aagccaccgc
     2341 cagttgaggt gatcacgacg tactgtcgtc catcgcggcc ttcataggtt atgggggttg
     2401 cctccgcggc gcctttcagt ttcacggtcc atagctcttt gccggtcctc ccgtcgaatg
     2461 cacggaagcg cgcatcgtca gtcgcgccga caaagaccag ccctccggcc gtcgcgatgg
     2521 tgccgccgtt tccgggacgc ccagtattgc gcttgtcttc aggcaggctg tcggttacgc
     2581 caagtggtac cctccacgcg atctcaccgg tattcacatt gactgccgcg agttctcccc
     2641 agggcggttg ttggcaagga agctgctgcg tgcctggtcc cttgacgctg aatcttcccc
     2701 cgcccggaat gccctcgtag ggaccgtcgg gatcgacccg gttgccttgc ccgttcgcca
     2761 tcgctggacc gttcttcgga tcgtggtcct tgaggcccga tatctgccct aattggttga
     2821 cgttcgcgaa cagatagccc agcgctggat cgaacgaggt gcccccccaa ttcacgccgc
     2881 cgtggttgcc cgggaattgc acacggagat ggttataact gaccggcaaa aaaggccctc
     2941 cgacttccat gccttggacg agctgcttgc aggcagattc gagttcgggc gtgatggttg
     3001 cgatatcggt cttgctgaat gtcattcgca caaggggcgg cggcttgagg ggaaacggct
     3061 gcgtcttcga ggggcgctcg agtgggactt cgctgggagg tacggggcgc tcctcaacgc
     3121 cataaatcgg ttttccggtg acgcgattca gcaaaaagac cattccgatc ttatccatgg
     3181 caactacggc gggaatggtt ttacctccct gctttacgtc aatgagcgct ggagcgcccg
     3241 tgagatcagc gtcccagata tcgtgatgga ccacctggaa gtgccacaga tactttcctg
     3301 tgttggcgtc ggctgcaacg aggctggagc tgaagagatt atcgcccgcg cggtcaccgc
     3361 catactggtc tacggaaggc gcgccgaagg gcatgtatac gataccgcgc ttttcgtcga
     3421 ccgtcaggaa gccccagaca ttgacgcctg cgcgattttt ccaactgtcg tcagcccagg
     3481 tgtcgtgata cttttcgccc ggctggggaa tggaatggaa cgtccagacc agctttccgg
     3541 ttcgcatgtc ccaggcgcgg acgtcgccgg ccgggccatg aggcggattt tcctgcgaag
     3601 taccaccagt gatgacaagg ttcttgtaca cgatgggcgg ggatgacaga gcgttgcggc
     3661 cgggcaggcc gtgcatgatg gcctcggtat tgagattgat cacgccgttg tcgccgaaag
     3721 cagagttcgg ttttcccgtt ttcgcatcga gcgagtacag tttcccgtcc ctggacccga
     3781 agacgatttg agcgggagta tggccatcac ccgcccaata ttcaagcccg cgcgtggatg
     3841 ggttactgga ggggagccga taggtccaga tttctttgcc gtttacagga tcgatcgccg
     3901 caacgcgcga gtacggagtg gcgacataca taagcccgtg aatcacgagc ggtgtatctt
     3961 cgcttggacg gaagcgcgag ccgaatgcga agcggccgcc acccggagtt cgcggttcgt
     4021 caccaactgc tccgccgcgg ccctgcattt ttggtgcagc cgagccgccg cctccggcgc
     4081 tgctaaaacc gggcggcctc atgtgataca cccacgctac tttaagccgg ctgacatttt
     4141 cgggggtgat ttggtcgagg ggtgagaacc gcataccgcc tggatcatga ccgtacgtcc
     4201 gccactcctt tgccgcatcc accaccagac caccgggagg aagactggct tccttcctat
     4261 tcgcagccct gacaggaggg aaagcgcttt ctacgtttgg cgaagcggcg ccagtcgttg
     4321 ctgtatgatt tgttggctgc ccttttatcg gagatggggc attaatgccg cttgggccgg
     4381 gaatgggtcc atagaacttc acgagatagg aaacaatgcg attgaaatcg tcgggactac
     4441 cttgcgcgcc gaggttgatc atcttcgaaa tcgtatctgt ccaggccgcc tgtccctgac
     4501 gcagagatgt ggcttgctca agtgagtggc attgggagca aatcctgacc gtttccgctt
     4561 tgccggctcc attcggtaga
//
LOCUS       DATNDT010000082         5301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1394906, whole genome shotgun sequence.
ACCESSION   DATNDT010000082 DATNDT010000000
VERSION     DATNDT010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5301
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1394906"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2229)
                     /gene="uvrA"
                     /locus_tag="VN788_02495"
     CDS             complement(<1..2229)
                     /gene="uvrA"
                     /locus_tag="VN788_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXU19432.1"
                     /translation="MSLRKIVVRGARQHNLKNIDLEIPRNTLTVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLDQMERPEVDSVEGLSPSIAIEQKTTTRSPRSTVGT
                     ITEIYDYLRVVYSAIGTPHCPRCGKPIARQSTEQIVQAVMALKPDDRIMVLAPVVRGR
                     KGEYKKELEKYAKEGYVRARVDSELISLDEPVPLDRRKNHTIEIVVDRLLLKSGIVQR
                     LEQSIETALKLAGGLVTIAVVGGEEHVYSEKLACPDCGISIPQLEPRSFSFNSPYGAC
                     PECNGLGSKYEFDPAKVIVDWTKPLFEGGLGPGSGSTPLQRTLRIAALAHGFDLDAPF
                     EKLPRKVQNLILHGNNSDALGKGLNFEGIVGYLESSLRESKSDSYREWLTEYMSPAPC
                     TSCGEQRLRPESLAVKIGGLSVADFTSLALSDARAAVDKMRNGLTLRQRKIAGRPLSE
                     ISERIDFLLNVGLGYLTLARSAATLSGGEAQRIRLATQIGSRLRGVLYVLDEPSIGLH
                     PRDNNRLLSTLETLRDLGNTVLVVEHDEETIRRADYVIDLGPGAGKAGGHLVAAGSPA
                     EIAANSASLTGQYLTGQLKIAVPVQRRAANGKTLTILGAHSHNLKNIDVAIPLGLLSV
                     VTGVSGSGKSTLVNDILYHALAAKLYRSMEKPGAHRALVGAEHIDKVVQIDQAPIGRT
                     PRSNPATYTGVFAPIRELYAMLPESRERGYKPGRFSFNVKGGRCEACQGDGQRRIEMN
                     FLP"
     gene            complement(2390..2953)
                     /locus_tag="VN788_02500"
     CDS             complement(2390..2953)
                     /locus_tag="VN788_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016490794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXU19433.1"
                     /translation="MCIVEIETERLILRTWQISDRAEFARLNSDFRVMEFISACLSPA
                     ESDLFLDRILEHFRNHGFGLWAVELREERRFIGALGLMVPVFQAHFTPCVEIGWRLAA
                     DYWGRGLATQGARAVAHHAFESLHLESLVSFTAPANLRSRRVMEKIGMTRDSSDDFEH
                     PNLPEAHPLRPHVLYRLSRSNWPRARR"
     gene            3280..3355
                     /locus_tag="VN788_02505"
     tRNA            3280..3355
                     /locus_tag="VN788_02505"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3313..3315,aa:Asn,seq:gtt)
     gene            complement(3391..3762)
                     /locus_tag="VN788_02510"
     CDS             complement(3391..3762)
                     /locus_tag="VN788_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015879093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXU19434.1"
                     /translation="MDHAQGFLNLVNDAKQRIKQETFRETKKRLDNGEKLILVDTRED
                     AEWARGHIPGAIHLGRGIIERDIEKAIPDKGAPIVLYCGGGFRSALAADNLQKMGYRN
                     VISMDGGWRGWTEAGFPVTKD"
     gene            complement(3858..5132)
                     /locus_tag="VN788_02515"
     CDS             complement(3858..5132)
                     /locus_tag="VN788_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19435.1"
                     /translation="MANRGGFGEFKEAIGLALDVLRRNVLRSFLTILGIMIGVTTIIV
                     IGAVINGLNSNVMGNIQSLGSSTIIVSKFSWATLGRLSPKVLQRKDLNPDWVKDLTQL
                     PHVVAAAPVSQIENYAVNAGSSEVRRGNLRAKNVILQGNTPDIAKISDLDLAAGRFFN
                     GTDEQHHSPVTVLGHITANTLFPSGESPVGQEVLLEGHDFTVIGVLDERKQALGTGEN
                     PEDNIALVPFSVIRKLHPEFKNVVFMAKADTTENLPLVVDEVREYMRRERKLASDQDD
                     DFAILTTDTFLDLWKQISGGIFIVMLAVGSVALLVGGIGVMNIMLVSVTERTREIGVR
                     KAIGARRANILLQFLLEAVTLAGIGGIIGVLLGSAIVLCIRLAFSSFPASVSVLLVLL
                     GVSVASMIGIFFGVYPAWKAARLNPVDALRYE"
     gene            complement(5148..>5301)
                     /locus_tag="VN788_02520"
     CDS             complement(5148..>5301)
                     /locus_tag="VN788_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19436.1"
                     /translation="VSMTTALPSSVQIWSVLMGLIVATSVGLFFGVYPAAKAARLDPV
                     VALRSE"
BASE COUNT         1164 a         1558 c         1438 g         1141 t
ORIGIN      
        1 gggaagaaaa ttcatttcga tgcgccgctg cccatctccc tggcacgctt cgcaacgtcc
       61 gcctttcaca ttgaaactga agcggccggg cttatagccg cgctcgcgcg attccggaag
      121 catcgcatac aactccctga taggcgcgaa gacgccggtg taggtcgctg gattcgagcg
      181 cggagtgcgg ccgattggcg cctgatcaat ttgcacaact ttatctatgt gttccgcgcc
      241 caccagcgcg cgatgcgcgc ccggcttttc catcgaacgg taaagcttgg ctgcaagggc
      301 gtgatagaga atgtcgttca caagcgtcga tttccccgaa ccagaaacgc cagtcacaac
      361 ggacagcagc ccaagaggta tggccacatc gatgtttttg agattatggc tgtgagcccc
      421 gaggatcgtg agagtcttgc catttgcggc gcggcgctga accggcacag cgatcttcaa
      481 ttggcccgtt aggtattgtc ccgtaagaga ggccgagttt gccgcgattt ccgcgggcga
      541 accagccgct accagatgcc cgccggcctt gccggcgccc gggccgaggt caattacgta
      601 atcggcacgg cgaatcgtct cctcgtcatg ctcaacaacc aggacggtat ttcccaaatc
      661 gcgcagagtt tccagagtgg aaagcagacg attgttatcg cgaggatgca ggccgatgga
      721 aggctcgtcg agcacgtaca gcacgccccg gaggcgcgat ccgatttgcg tggccagccg
      781 aatgcgctgc gcctcgcctc ctgaaagggt tgccgccgaa cgcgcgagcg tcaggtatcc
      841 cagccctacg ttcagcagaa aatcgatgcg ctcagaaatt tctgatagcg gccgtccggc
      901 gatttttcgc tgcctcagcg tgagtccatt tcgcattttg tccacggccg cccgcgcatc
      961 cgatagcgcc agggacgtga aatcggcaac ggaaagacct cctattttca cggccaagct
     1021 ttcgggacgc agccgctgct caccgcaaga ggtgcaggga gcgggtgaca tgtattccgt
     1081 caaccactcg cgataggaat ccgatttcga ttctctcagg ctgctttcca aatatccgac
     1141 gataccttcg aaattcaaac ccttgcctaa agcgtcggaa ttatttccgt gaaggatcag
     1201 attctgcacc ttgcgtggaa gtttttcaaa aggcgcatcg aggtcgaatc cgtgggcgag
     1261 agcggcgatt cgcaaagtgc gttgcaaggg agtggaaccg gagcccggtc cgaggccgcc
     1321 ttcaaaaaga ggcttggtcc aatccacgat caccttcgct ggatcaaatt catatttcga
     1381 tcccagccca ttgcattccg ggcatgcgcc gtagggcgaa ttgaagctga atgaacgggg
     1441 ttcgagttgt ggtatggaga ttccgcaatc ggggcaggca agtttttcgg aataaacgtg
     1501 ctcctctcct cccaccacag cgatcgtgac caaaccgccc gcgagcttca aagcggtttc
     1561 aatggattgc tcgaggcgct gcacaatgcc gctcttcagc agcaaacgat ccaccacaat
     1621 ttctatggtg tgatttttcc ggcgatccag cggcacaggt tcgtccagac taatcaactc
     1681 gctatccacg cgggcgcgga cgtaaccctc tttcgcatac ttttcgagtt cctttttgta
     1741 ttcgcctttg cgtccgcgga ccacaggtgc cagaaccatg atgcgatcat cgggcttgag
     1801 tgccatcacg gcttgcacga tttgctccgt ggattggcgc gcaatgggct tgccgcatcg
     1861 tgggcagtgc ggcgtcccga tggcgctgta aactacgcga agataatcgt aaatctcggt
     1921 gatcgtcccc acggtcgatc gcggcgagcg cgtggttgtt ttctgctcga tggcgatcga
     1981 cggtgagagt ccctcaaccg aatcaacctc cggccgctcc atttgatcaa ggaactggcg
     2041 cgcgtaagct gaaagagatt cgacgtagcg ccgctggccc tctgcgtaga gcgtgtcaaa
     2101 agcaagcgag gatttacccg aaccagaaag tcccgtgacc accgtgaggg tattgcgcgg
     2161 aatttccagg tcgatgttct taaggttgtg ctgccgggcg ccccggacga cgatttttct
     2221 tagactcatg cctcgattgc cgatacagag agaaccgcca cacactgggt agactgatcc
     2281 cagtgtaact ttttaacata gcaaccgcca cgcagtgagg tcaaggcagc gcgactgtgg
     2341 attcgtctgc aattgaggtt gaaatcaacc gcgcagcggc gagggttcac tatcgccgag
     2401 cgcgcggcca attgcttcgg ctcaggcgat acaggacgtg cggccgcagc ggatgagcct
     2461 ccggcaggtt tggatgttcg aagtcgtccg atgaatcgcg agtcattccg attttctcca
     2521 tcacgcggcg cgagcggagg ttcgccggag cggtgaaaga aaccaaggat tccagatgca
     2581 gcgactcgaa tgcgtggtgc gctaccgccc tcgctccttg ggtggcgagc ccgcgtcccc
     2641 agtaatcggc cgctagtcgc cagccgatct cgacacatgg agtgaaatgg gcttggaaca
     2701 caggcaccat taatcccagt gctccaatga atcgtcgctc ttcgcgcagt tcaaccgccc
     2761 acaatccgaa accgtggttg cggaagtgct ccagaattcg atcgagaaaa agatcgcttt
     2821 ccgcaggcga aaggcaagcg gagatgaatt ccatcactcg aaaatcggaa tttagccgcg
     2881 caaactccgc cctgtcgctt atttgccacg ttctgaggat gaggcgttct gtttcaatct
     2941 ccacaatgca cattttctag gccagctcgc tacagcgaca gactgtggat tcccagcttt
     3001 gccccggtga cgcgtcgaaa aatttactgg gggctgggag tttttacgca agccgcaatt
     3061 ttgacgacgt ttggtttcag catcttaact ttggttttga gcgtgcgata tatgggcccg
     3121 tcagagggaa ggaaagccga ggatcccttt aggggattga gggctgggcc ggcttccttc
     3181 cctcggccat ttaccttagt actctgtagg ttcccaccaa tccgcgttca cctccgcgac
     3241 agaatcgtgt aaattattac acttagtttg cggccgagtt ggggcgtggt ccaatcggta
     3301 gggcacctca ctgttaatga ggacggtgtt ggttcgagtc caaccgcccc agccaactct
     3361 tttcggtcgc cggacggacg gatcgcaaac ttaatccttg gtgacgggaa agcccgcttc
     3421 ggtccaaccg cgccagccgc cgtccattga aatcacattg cgatagccca ttttctgaag
     3481 gttgtccgcg gccagggctg aacggaatcc gccgccgcaa tagagaacga ttggcgcgcc
     3541 tttatctgga atagctttct cgatatcgcg ctcgatgatg ccgcggccca gatgaattgc
     3601 gccgggaatg tgcccacgag cccattcggc gtcttcgcgc gtatccacca gaataagttt
     3661 ctcgccgtta tccagccgtt tttttgtttc gcgaaatgtt tcttgcttga ttcgctgctt
     3721 ggcatcgttt acgagattca aaaatccttg ggcgtgatcc atttttcacc tcatcctata
     3781 ggttagctta gagattttag cacccgggat tcgagcgaac attgcgtgag agcaaaacca
     3841 gttagcgcga cgccgaccta ctcgtagcgc agtgcgtcca ccgggtttaa tcgagcggcc
     3901 ttccaggctg gatacacacc gaagaaaatc ccgatcatgc tggctacgga tactccaagc
     3961 aggacaagca acacggaaac ggacgcaggg aaactggaaa aagccagccg gatgcaaaga
     4021 acgatggcag agccgagaag cacaccaatg atcccaccga tccccgcaag ggtgaccgcc
     4081 tccagcagaa attgcagcag gatattcgcc cgccgcgcgc caatcgcctt gcggactcca
     4141 atttcgcgcg ttcgctcggt aacgctcacg agcataatat tcataacgcc gattccaccg
     4201 accagcagcg cgacggatcc gaccgcaagc atcacaatga aaattccgcc ggagatctgc
     4261 ttccacaaat ccagaaacgt gtccgtggtg aggatcgcga aatcgtcatc ctggtccgaa
     4321 gccagcttgc gctcccgacg catatattcc cgcacctcgt ccaccaccag aggaagattt
     4381 tccgtggtgt ccgccttggc catgaagacc acgttcttaa actcgggatg cagcttccgt
     4441 attactgaaa acggcaccag cgcgatattg tcttcgggat tctccccggt ccccaatgct
     4501 tgcttgcgct cgtccaaaac gccgatcacg gtgaagtcgt gcccttcaag caacacttct
     4561 tggcctacgg gactctcccc gctggggaaa agcgtattgg ctgtgatgtg ccccagcacc
     4621 gtaaccgggg agtggtgctg ttcatccgta ccattgaaaa acctgcctgc ggcgagatcc
     4681 aaatcgctga tcttcgcaat atcgggcgta ttcccctgca ggatcacatt cttcgcgcgg
     4741 agattccccc gccgcacttc gctcgagccg gcgttgaccg catagttttc gatctgcgaa
     4801 acaggagccg cggcaaccac gtgcggcagt tgcgtgagat ctttcaccca atccggattc
     4861 agatccttcc gctgaaggac tttcggcgaa aggcgtccga gtgtggccca cgaaaatttc
     4921 gagacgataa tggtgctcga gcctagcgac tgaatgttgc ccatcacatt cgagttcagc
     4981 ccgttaatca cggctccaat cacgatgatc gtggtcacgc cgatcatgat gccgagaatc
     5041 gtgaggaatg aacgcagcac gttgcgccgc aacacatcga gcgccagccc aatcgcttcc
     5101 ttgaattcgc cgaacccgcc cctatttgcc atcgttcctt caaccaacta ttccgaccgc
     5161 aatgccacaa ccggatcgag ccgcgccgcc ttcgccgccg gatacacgcc gaagaacagg
     5221 cccacgctgg tggccacgat caatcccatc agcaccgacc aaatctggac cgagctcggc
     5281 agcgccgtcg tcatgctcac a
//
LOCUS       DATNDT010000083         2179 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1770587, whole genome shotgun sequence.
ACCESSION   DATNDT010000083 DATNDT010000000
VERSION     DATNDT010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1770587"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2179
                     /gene="uvrA"
                     /locus_tag="VN788_02525"
     CDS             <1..>2179
                     /gene="uvrA"
                     /locus_tag="VN788_02525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXU19437.1"
                     /translation="FSTPESLLELNFAEPVFALVDRIVFSPDQRARIVDALEIAYREG
                     GEAIFEIVPRDDAPRRNLRFSQRFECKNCGRRYEEPEPRLFSFNNPYGACQRCQGFGN
                     TIDFDLKLVIPNPTLPLAAGAIEPWTKPKYRGFQTELRKFAKAHDIPTDVPWCELDGD
                     QRLLITQGEGKFSGITGFFSLLERKKYKLHVRVFLSRYRGYATCQECQGQRLRAEARS
                     VMIDGKNICEVTAMTVEEAAKFIAGVTLTQHQAEVAERILHEIRDRLGFLNSVGLEYL
                     TLDRTASTLSGGESQRIQLATSLGSRLVGALYVLDEPSIGLHSRDTERLVRILHELRD
                     LGNTILVVEHDAEVMAASDRIVDLGPGAGELGGRIIASGSFPEISRCSQSLTGRYLSH
                     NLSIPVPAVRRKSAGKIRVIGARANNLKAIDVTIPLGMLVAITGVSGSGKSTLVHDVL
                     YKALAPAGSPESGNSAGSFQRLEGAEQIREVVLVDQSPIGRTPRSNPVTYIKAFDAIR
                     ELFASLPEAQKRGYSAGHFSFNVPGGRCEACQGDGTVTVEMQFLADVELICEECRGTR
                     YKSSVLEVRYKGKNIHDVLNLTVKEGLQFFSGVPKIVDKLRVLEEVGLGYLRLGQSAT
                     TLSGGEAQRMKLAAHLTPRSREEQERNRESPNRNPGAGRPLPKRTLYIFDEPTTGLHF
                     DDVSKLLAAFRRLIDAGGSLVVIEHNLDVIKAADWVIDLGPEGG"
BASE COUNT          445 a          617 c          655 g          462 t
ORIGIN      
        1 gttctctacg ccggaatctc tgctggaatt gaacttcgcg gaaccggtgt ttgccttggt
       61 tgaccgcatc gtcttttcgc ccgaccagcg cgcgcgaatc gtcgatgcgc tggagatcgc
      121 ctatcgcgaa ggtggcgaag cgatcttcga gatcgtgccg cgcgatgatg cgccacgcag
      181 gaatctgcgg ttctcccagc gtttcgaatg caaaaattgc gggcgccgtt acgaagagcc
      241 ggagccgcgg ctgttttcct tcaataaccc ttacggagcg tgccagcgct gccagggctt
      301 cggcaacacg atcgatttcg acttgaagct ggtgattcct aatcccacgc tcccgttggc
      361 tgcgggggca attgagccct ggacaaagcc gaaatatcgg ggttttcaga cggagctcag
      421 aaaattcgcc aaagctcacg acattcctac tgacgttccc tggtgcgaac tcgatggcga
      481 tcagcgtctc ctgattacgc aaggcgaagg aaagttctcc ggcattacag gattcttcag
      541 cctgctggag cgcaagaaat acaagctgca tgttcgggta tttctcagcc gctaccgtgg
      601 atacgccacg tgtcaggagt gtcagggaca gcgccttcgc gccgaagctc gtagcgtgat
      661 gattgatggc aagaacattt gtgaggtcac tgccatgacc gtggaagagg cggcgaagtt
      721 cattgcgggc gtgactctca cgcagcatca ggccgaagta gccgagcgaa tcctccacga
      781 aattcgcgat cgtctgggct ttctcaacag cgtcgggctc gaatacctca cacttgaccg
      841 cactgcttcc accctctccg gcggcgaatc gcaacgcatt cagttggcga cttcactagg
      901 ctcgcggttg gtgggggctc tctacgttct cgatgagcct tcgattggat tgcacagccg
      961 cgataccgaa aggctagtgc gcattcttca cgaactgcgc gacctgggaa atacgattct
     1021 ggtggtggag cacgatgccg aagtcatggc tgcttcagat cggatcgtcg atctcggtcc
     1081 gggagccggc gagttgggcg gcagaatcat tgcctcggga tcgtttcccg agatctcgcg
     1141 ctgtagccaa tctctgaccg gccgctattt gagccacaat ctctcgattc cggtgccggc
     1201 ggtgcggcgc aagtccgcag gaaaaattcg cgtgatcggc gctcgagcca acaacctgaa
     1261 ggccattgat gtgaccattc cattgggaat gctggtggca atcaccggcg tctcagggtc
     1321 ggggaaatcg acgctggtgc acgacgtgtt gtacaaagcg ttggccccgg ccgggtctcc
     1381 cgaatcggga aattccgcgg gatcgtttca gcggctggag ggggccgagc agattcgcga
     1441 ggtggtcctc gtggatcagt caccgatcgg acgcaccccg cgctcgaatc ccgtgaccta
     1501 catcaaggcc ttcgacgcca tccgcgagct cttcgcttcc cttcccgaag cgcagaaacg
     1561 cggatacagc gccggccatt tctcctttaa cgttcccggc gggcgctgcg aggcttgcca
     1621 gggcgatggc accgtcaccg tggaaatgca atttctcgcc gatgtggagc tgatctgcga
     1681 ggagtgccgg ggcactcgct ataagtcttc agtcctcgag gtgcgctaca agggcaagaa
     1741 catccacgat gtcttgaatc tcaccgtaaa agagggcctc cagttttttt ccggagtgcc
     1801 caagattgtg gacaagcttc gggttcttga agaggtggga ctgggatatc tccggctggg
     1861 gcagtcagcg actaccctca gcggcggtga agctcagcgc atgaaattgg cggcacacct
     1921 gacgccgcgt tcgcgcgagg agcaagagcg gaatcgagag tcccctaaca ggaacccggg
     1981 agcaggacgg ccattaccga aacgaaccct gtatattttt gacgagccta ccactggtct
     2041 gcatttcgat gatgtaagca agctgctcgc agcattccgc cgcctcatcg acgcgggcgg
     2101 gtcgctggtg gtaattgaac ataatctgga tgtgatcaaa gccgccgatt gggtcatcga
     2161 tctcggtcca gagggaggc
//
LOCUS       DATNDT010000084         4207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3495459, whole genome shotgun sequence.
ACCESSION   DATNDT010000084 DATNDT010000000
VERSION     DATNDT010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495459"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            452..652
                     /locus_tag="VN788_02530"
     CDS             452..652
                     /locus_tag="VN788_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXU19438.1"
                     /translation="MEQGTVKWFNDAKGFGFISRQSGDDVFVHFSAIQAGGFRSLQEG
                     QQVEFNVTKGPKGFQAEGVRPL"
     gene            815..1765
                     /locus_tag="VN788_02535"
     CDS             815..1765
                     /locus_tag="VN788_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXU19439.1"
                     /translation="MRSFAIAFMACAILSIVSGQSGVPPATRVHVVMLGTGTPLPDPE
                     RSGPSIAVVVDNHAYLFDAGTGVVRRAAAATRQKGIAGLEPRTLRVAFLTHLHSDHTL
                     GLPDLILTPWTMGRTEPLELWGPKGTRAMVEHILAAYREDIAIRTTSLEGSNATGYKV
                     NVHEIAPGDVYKDGIVTVKPFLVKHGAWKQALGYRIEAPGRVIVYSGDTSRSESIVES
                     CHGCDYLISEAYTLASFDLVSPHWQTYRRAYHASTRELGDIATRAKPRLLILTHRGNA
                     GCDQSQAAGCLESGSEEQMLKEVKHYYSGNVLAAHDLDVY"
     gene            complement(1977..2909)
                     /locus_tag="VN788_02540"
     CDS             complement(1977..2909)
                     /locus_tag="VN788_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXU19440.1"
                     /translation="MEHLSRRTFLAHTIVVASAPLLPALDGASTKTRLILLGTGGGPR
                     PRKARSASAQVIITNNTAYVIDCGSGVARQLVFADVPLPIVRHVFLTHHHSDHNADYG
                     NLIWLAWESGLSTRVDTWGPPPLDTMTKLFFEMNAYDIETRIKNEGRVPIGPLVHVHE
                     KSEGGSVMQDENVRVTATLVDHPPVFPAFAYRFDASDRSIVISGDTRPCENLVKLAQK
                     ADVLVHSAMYVPALDRLVARVPNARTLKASILAHQTSAEDAGRIAQAAAVKTLVLSHL
                     VPPDDPEVTDQMWIDAAHKYFHGPIIVGRDLLEI"
     gene            3099..3641
                     /locus_tag="VN788_02545"
     CDS             3099..3641
                     /locus_tag="VN788_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009281456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXU19441.1"
                     /translation="MQISIDGMVAAQKGKTFFNWDDEVRRYSIANAANADCILLGRKT
                     AMGFIPHWKSVADNPKDADFEIGKVITDIPKVVFSRTLHKSEWPNASIANGEIVDSVN
                     QLKQQTGKDLLVYGGTSFVASLIGHNLIDEYHLLVNPIALGSGQTIFSSLADTLRLNL
                     VFNRAFSCGTVLLHYESIRD"
     gene            3891..>4207
                     /locus_tag="VN788_02550"
     CDS             3891..>4207
                     /locus_tag="VN788_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018481971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU19442.1"
                     /translation="MDNVGIVVDDLGATIDFFRELGLELEGRAMIEGEWAGRVTGVRD
                     QRVEIAMMRMPDGHGRLELSRFLSPPVVADHRNAPVNALGYLRVMFAVDDIDETLERL
                     RKRG"
BASE COUNT          948 a         1156 c         1157 g          946 t
ORIGIN      
        1 ctctgaaatg atttcgcgaa tatccaaagg gttagtttct cctttttcac tgagcatttt
       61 acttgaacac gatatacgtg ctcttgaata cgcaaagaaa cgccccaatg cgaaaatggt
      121 ggagcttcat ggctattgcc aagggccacg cggaagggtc gcggcacagg ctgtgccgat
      181 cccatctatc gagccctttg cgaggaagcc gccttcgata cgcatcgtcg tggtacgcgc
      241 gtcagccgag ttctcacgag attcgattgc ccgcgtttgt taagtctcgg caaaacagaa
      301 tcgcaatgaa tcgtttgcct cacatcgcac atctaattaa tatcagctaa gtttgattca
      361 cctgcagtga cagtttaatt tcgcgacagc gttctctctc acccgcaccg actcttctaa
      421 gcagaaattc aaaatagaaa caggtaacac aatggaacaa ggaacagtga aatggtttaa
      481 cgacgccaaa ggcttcggct tcatcagccg ccagtctggc gacgatgttt tcgtgcattt
      541 ttcggccatc caggcaggcg gctttcgcag cttgcaggaa ggccagcaag tcgagttcaa
      601 cgtgacgaaa ggtccaaagg gcttccaggc agaaggcgtc agacctctct aagccgaaag
      661 caagcaactt gcccggagcg atcactcgct gccgggcttt ttctgcgacg aattccccga
      721 caccggcagt tttgtcgatg cactgggttg tggggcgagg attccctgtg caacgcaccc
      781 accagctaca atcttgcact gagcggaggc agcgatgcga agtttcgcga ttgcattcat
      841 ggcgtgtgct attttgtcga ttgtgtctgg tcaaagtggt gtaccaccgg cgacccgcgt
      901 tcacgtcgtt atgctgggta ccggaacacc gctgccggat ccggagcgct ctggaccgag
      961 catcgctgtt gtggtggaca accatgcata tttgttcgat gctggcacgg gcgtcgtgcg
     1021 ccgggccgcc gcagcgacaa ggcagaaggg catcgcaggc ctggaaccgc gcacactgcg
     1081 cgtcgctttc ctgacacatc tccattccga ccacacactc ggtctgcccg atctcattct
     1141 cacgccttgg acgatgggcc gcaccgaacc ccttgagctt tggggtccta aaggcacacg
     1201 cgccatggtc gagcacattc tcgccgccta ccgggaagac attgcgatcc gtaccaccag
     1261 tcttgagggc tcgaatgcaa ccggctacaa agtgaacgtg catgagatcg cacccggcga
     1321 tgtctataaa gacggtatcg tcaccgtgaa accattcctg gtgaagcacg gcgcgtggaa
     1381 gcaggctctc gggtatcgga tcgaagcacc ggggcgcgtc atcgtgtatt ccggcgatac
     1441 cagccgcagt gagagcatcg tcgagagttg ccacggttgc gactatctga tctctgaggc
     1501 ctacaccctc gccagctttg atctcgtctc cccgcattgg caaacatacc gtcgcgccta
     1561 ccatgcctcc acacgcgagc taggcgacat cgccacccgt gccaaacccc gcctgctcat
     1621 acttacccat cgcggcaacg ccggctgcga ccagtcccag gctgcgggct gcctcgagtc
     1681 cggaagtgaa gaacagatgt tgaaggaggt gaagcactac tattcgggga acgtgcttgc
     1741 agcacacgac ctggatgtgt actaggctgg catgtgcccg cagctccgcc gaactgtcgg
     1801 cgagggcgga caactttacc tctctgtata gtcggtcaac aggaagtaat ttggttattt
     1861 ccggcttgcc cgtcattcac caagggtcac taccgggctc cctgtgtagc cgcggagcgg
     1921 caacagtctg ttctctgttc acttggacaa atcaagggtg agcactattc gaccaatcaa
     1981 atctccagca aatccctgcc gacaatgatc gggccatgaa aatatttgtg ggccgcatca
     2041 atccacattt gatccgtgac ttcaggatca tcaggcggca cgagatgaga gagcaccaga
     2101 gttttgaccg cagcggcctg cgcaattcga cccgcatctt ccgcggaagt ctgatgagcc
     2161 aggatgctgg ctttgagggt cctcgcgttc ggtacacgcg caacgaggcg gtcaagcgcg
     2221 ggcacgtaca ttgcagagtg caccaggacg tcggcctttt gcgcgagttt caccaggttc
     2281 tcgcatggtc gtgtatcgcc ggaaataacg atcgaacggt ccgaagcgtc gaagcgataa
     2341 gcgaatgccg gaaaaaccgg cgggtgatcc actagggttg ccgtcaccct tacattctcg
     2401 tcctgcatga ccgaaccacc ctcactcttt tcatgtacat gaacaagtgg tccaataggc
     2461 actcgcccct cgtttttgat tcgagtttcg atgtcataag cattcatctc gaagaacagt
     2521 ttggtcatcg tgtcgagggg tggcggtccc caggtgtcga cacgcgtgct gagcccactc
     2581 tcccaggcaa gccaaatcag gtttccataa tccgcattgt gatcggagtg gtgatgcgtc
     2641 aggaagacat gccgcactat gggcagcggc acatcggcaa agacgagttg tcttgcgacg
     2701 ccgctgccac aatctattac gtacgctgta ttgtttgtaa tgatgacctg tgcggaagcc
     2761 gacctcgctt ttcgtggtct tgggccgccg cccgttccga ggagaattag ccgggtcttc
     2821 gtactcgcgc cgtccagggc cgggagtagc ggagcgctag ctacaacaat tgtatgtgcc
     2881 aggaatgtgc ggcggcttag atgctccatt agtgggactc cgtcgggctc tcattgtatg
     2941 gcaatggaaa acctcgccgt ttcattaacc gggttcttgg tagagcgagt caaagttaaa
     3001 ctcttttgga tatacttgtc gatttccgct gtggccgttc gactaatcaa tagagggaca
     3061 agaaaatgga agaacggacg agaaggctga aattgcaaat gcagatatcg atcgatggga
     3121 tggtggcggc gcaaaagggc aagacatttt tcaattggga tgacgaggtc aggcggtatt
     3181 caatcgcgaa tgcggcgaat gccgactgta tcctcctcgg gcgcaaaacc gcaatgggct
     3241 tcatccctca ctggaaatcg gtagccgaca accccaaaga tgccgatttt gaaataggca
     3301 aggtaattac agacataccc aaggttgtct tctcaagaac tcttcacaaa tcggaatggc
     3361 cgaatgcgag catagcgaat ggcgagatcg tcgattctgt gaatcaactg aagcagcaaa
     3421 caggcaagga ccttcttgtg tatggtggaa ctagtttcgt cgcatcctta atcgggcaca
     3481 accttatcga tgaatatcac ttgcttgtaa atcccattgc gctcggaagc ggccaaacga
     3541 tcttctctag tttggctgat accctacgcc tcaatctggt attcaaccga gcattttctt
     3601 gcggaacggt gttgctgcat tacgaatcaa ttcgtgacta ggctctagaa atggttttgg
     3661 caggtcctcg gcaggcgtgc agtcgctaac agttgattgc agtgaaccac tcatgctcgc
     3721 ggtgccaagt agatatacgc caccttccga atagctcgtg atccgccccg agtaagctat
     3781 ttgggagcga tgtctcaggc cccaagctgc tgttgcttcg gcgttgctga catgctccat
     3841 tgtcagattt aaacggcacc ttgtagattg gacacacggc gctcaaacgg atggacaacg
     3901 tgggcatcgt cgtcgatgac ctcggagcga cgattgattt ctttcgcgag ctcggcctcg
     3961 agctcgaagg acgggccatg atcgaaggag aatgggccgg acgtgtcacg ggagttcgcg
     4021 atcagcgggt tgagattgcc atgatgcgca tgccggacgg ccatggccgg ctcgagctct
     4081 cccgcttcct cagcccgcct gtcgtcgcgg atcaccgcaa cgctccggtg aacgctctag
     4141 gctacctccg cgtcatgttc gccgttgacg acattgatga gacgcttgaa aggctgcgta
     4201 agcgggg
//
LOCUS       DATNDT010000085         2940 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_343794, whole genome shotgun sequence.
ACCESSION   DATNDT010000085 DATNDT010000000
VERSION     DATNDT010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2940
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_343794"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1435
                     /locus_tag="VN788_02555"
     CDS             23..1435
                     /locus_tag="VN788_02555"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU19443.1"
                     /translation="MGAIAGQATVAVTRKNLSVCVLDSDPGQLKLATNALERAGFQAV
                     GTTSPAEALQTVRTGGCRVVMIDCKMPAMDGFTFLEKTLQHDPGMPVLFTGFYSVESA
                     IEAIKRGAYDYLCKPVDLPRLVRTLDDLAELFWQRSEIRHLEDQLLQNLHFQGIVAKS
                     PAMMNVFDLVKKISRHYTNVLITGPTGAGKEPVARALHQLSPVAQEQFAVCNCSALVD
                     TLLESQLFGHIRGAFTGATDTKPGLFEYANGGTVFLDEIGETSLPLQAKLLRVIQNRE
                     IQCVGSPELKRVNVRIVAATNRDLRAEVLAGRFREDLFYRLSSIEIGVPGLAERPEDI
                     PVLVQHFLKKYGQAYGKKLQGLSRRAQLVLRDHNWPGNVRELENVISSAAIVATADFI
                     DVGDLPEHLRKPQRNGSNAVGDWQALPLDEIRSMHIQRVLDACHGNRVRAARMLGIGR
                     TSLYRFLKRTNKQSSPVLVG"
     gene            complement(1432..2202)
                     /locus_tag="VN788_02560"
     CDS             complement(1432..2202)
                     /locus_tag="VN788_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19444.1"
                     /translation="MNREGNKKIRALIVDDEDLARQVLREFISAHSEIEIVAECANGF
                     EAVKFATELEPDLLFLDIQMPKLDGFEVLELIGANRAVVFVTAYDTFAIRAFEVHAVD
                     YLLKPISADRFDAALERAKERLSGKLPPAPVPANDLAAAARPPSQYLDRIPVRDGTSV
                     FIIPVGKLDYAEAQDDYVALVSDGKKHLKQQTISSLEGALDPSRFLRIHRSYIVNLER
                     VAKIEPYGKDSHIAVLAGGTRLPVSRSGHARLRSFLDR"
     gene            complement(2199..>2940)
                     /locus_tag="VN788_02565"
     CDS             complement(2199..>2940)
                     /locus_tag="VN788_02565"
                     /inference="COORDINATES: protein motif:HMM:NF018305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXU19445.1"
                     /translation="DARLAKDFPLLFGTGVLLYLLAVGFHYVLLAVDASREAEARVMK
                     ASVLARDAELRALKAQVNPHFLFNSLNSISALTTSDAAKAREMCILLGDFLRRTLGLG
                     EKQSIPLEEEMSLVHAFLAVEKIRYGSRLQMEETVEEEALTALVPPLLLQPLVENAVG
                     HGIANLVEGGLIRLEAKQRDGYLSIAIENPFDPQAPQRRRNGVGLANVRQRLEARYGN
                     SATFGATTEDGRFRVALSLPAGGKERTA"
BASE COUNT          599 a          883 c          825 g          633 t
ORIGIN      
        1 tgtcgtgcct tagaaccgtc ctatgggagc cattgcgggg caggccacgg tcgccgtcac
       61 gcgaaaaaat ctttccgtct gtgtgctgga tagcgatcca ggtcagttga aactggcgac
      121 gaacgcactt gaacgcgccg gtttccaggc cgtgggtacc acaagtcctg cggaagccct
      181 ccaaaccgtg cgaacgggcg gctgccgcgt cgtcatgatc gactgcaaga tgcccgcgat
      241 ggatggcttc acctttctcg aaaaaacctt gcagcacgat cccgggatgc ccgtgctctt
      301 caccggcttc tattcggtcg agtccgcgat agaggctatc aagcgcggtg cttacgacta
      361 cctgtgcaag cccgttgatt tgccgcggct tgttaggacg cttgatgatc tcgcagagtt
      421 attctggcag cgctctgaaa ttcggcactt ggaagatcag cttctgcaaa atcttcactt
      481 tcaaggaatc gtggcaaaaa gtcccgccat gatgaacgta ttcgacctcg taaagaaaat
      541 ctcgcgacac tacaccaatg ttctgatcac cgggcccaca ggcgccggaa aggagcctgt
      601 cgcccgcgcg ctccaccagt tgagcccggt ggcgcaggaa caattcgccg tctgcaattg
      661 ctcggcgctg gtcgatacct tgctcgaaag ccagctgttc ggacatattc gaggggcttt
      721 cacgggcgcg actgatacca agcccggtct cttcgagtac gccaatggcg gcactgtctt
      781 cctcgacgag attggtgaaa cttcattgcc gctccaggcg aaattacttc gcgtcatcca
      841 gaatcgcgaa attcagtgcg tgggatctcc tgagttgaaa agggtaaacg tgcgaatcgt
      901 ggcggcgacc aatcgagacc ttcgcgccga agttctcgcc ggccgcttcc gcgaggatct
      961 cttttacagg ctcagcagca tcgagattgg agttcccggc cttgccgagc gtcccgaaga
     1021 tatccccgtt ctcgttcagc attttctgaa aaaatatggc caggcgtatg gaaagaagtt
     1081 gcagggactt tcgcggcgcg cgcagctcgt tttgcgagac cacaactggc ccggcaatgt
     1141 ccgcgagctc gaaaatgtaa tttctagcgc ggccatcgtt gccaccgccg attttatcga
     1201 tgtcggagac ctgcccgaac atctgcgaaa gccgcagcga aatggatcga atgctgttgg
     1261 cgactggcag gccctgcccc ttgacgagat tcgcagcatg cacatccagc gcgttcttga
     1321 tgcctgccac ggcaatcgcg tgcgcgcggc tcgtatgctc ggcatcggcc gcaccagcct
     1381 ctatcgcttc ctcaaacgca ccaacaaaca atcttcgccg gtgttggttg gctagcgatc
     1441 caaaaaggag cgaagcctcg cgtgtccgga gcggctgacg ggaagccgag ttccgccggc
     1501 cagcaccgca atgtgtgaat ccttgccgta cggctcgatt ttagccacgc gctccaggtt
     1561 tacgatgtac gaccgatgga tgcgcagaaa ccgtgatggg tcgagcgcac cttccaggct
     1621 cgatatcgtt tgctgcttca aatgtttctt gccgtccgac accagcgcca cgtagtcgtc
     1681 ctgtgcttcc gcgtagtcca gctttcccac cggaatgatg aagacgctgg tgccatcgcg
     1741 caccggaatt cggtcgagat attgcgacgg cgggcgggcg gctgcggcaa gatcgttcgc
     1801 cggaacgggc gccggcggca gcttcccgga gagccgttct ttcgcgcgtt ccagcgcggc
     1861 gtcgaagcgg tcggcgctga tcggtttcaa aaggtagtcc acggcgtgta cttcgaaggc
     1921 gcggatcgcg aatgtatcgt acgcggtgac gaacaccacg gcacgattcg cgccaatcag
     1981 ctccagcact tcaaagccgt ccagcttcgg catttgaata tccaggaaca agagatcagg
     2041 ctcgagttct gtggcgaatt tgacggcttc gaaaccgttc gcgcattccg ctacgatttc
     2101 aatctccgaa tgggcggaaa taaactcacg cagaacttgt cgtgcgaggt cttcgtcatc
     2161 cacaatcagc gcgcgaattt tcttgttccc ttcgcggttc acgctgttcg ttcctttccg
     2221 cctgccggta gactgagcgc cacgcgaaag cgcccatcct ctgtcgtggc gccgaatgtc
     2281 gcgctatttc cataacgtgc ttccagccgc tgtcgaacat tagcgagccc cactccgttt
     2341 ctgcggcgtt gcggagcttg cggatcgaac gggttctcga tcgcgatcga caaatatccg
     2401 tcgcgttgct tggcctcgag cctgatcaaa ccgccttcga cgagattggc gattccgtgc
     2461 ccgactgcgt tttcaaccag aggctgcagc aaaaggggcg gcactagcgc ggtgagtgcc
     2521 tcctcttcga cagtctcttc catctgcagg cgcgagccat agcgtatctt ttccacggct
     2581 aaaaatgcat ggaccaacga catttcttcc tcgagcggta tcgattgctt ttctccgagc
     2641 cccagcgtcc ggcgcaggaa gtcgccgagc agtatgcaca tttcgcgggc tttggcggca
     2701 tccgaagtgg taagggcgct gattgaattc aagctgttga acagaaagtg cggattcacc
     2761 tgcgctttca aggccctcaa ctccgcgtcg cgcgcaagca cgctcgcttt catcacgcgg
     2821 gcctcggctt cgcgcgaggc atccacggcg agaagcacgt agtgaaaccc gaccgccagc
     2881 aggtacagca aaactcccgt tccaaacaac agcggaaaat ccttcgcgag ccgtgcgtcg
//
LOCUS       DATNDT010000086         3149 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1774194, whole genome shotgun sequence.
ACCESSION   DATNDT010000086 DATNDT010000000
VERSION     DATNDT010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3149
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1774194"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="VN788_02570"
     CDS             <1..756
                     /locus_tag="VN788_02570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit A"
                     /protein_id="HXU19446.1"
                     /translation="IRELYAMLPESRERGYKPGRFSFNVKGGRCEACQGDGQRRIEMN
                     FLPDVYVTCEVCRGKRYNAETLSVRYKGCSISDLLDLPIEDALKVLENIPSIQQKLQT
                     LVDVGLGYVQLGQSATTLSGGEAQRTKLARELSRRQTGRTLYILDEPTTGLHFDDVSK
                     LLTVLNRLVELGNTVLVIEHNLDVIKQADWIIDLGPEGGQEGGRIVAQGTPEHVARNK
                     KSYTGQILARALNGSNSNGPGRSTNAAARQLSA"
     gene            764..1711
                     /locus_tag="VN788_02575"
     CDS             764..1711
                     /locus_tag="VN788_02575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family glutamic-type intramembrane
                     protease"
                     /protein_id="HXU19447.1"
                     /translation="MSAANPSPTMNFEENIPPPAQPSESFDGPSPQPEPAPLSTSQLP
                     QEGPPTESHIPVSPTLSLPEDIRVPWGWTDLLLFVVLALAATFLFSILLAIGFAVFGV
                     PLSRLQKPGSAEGLFVVVSQAILFFVLLGYLFIQARVRFHAPFWRTLGWRSLETHRIR
                     RGWAYLGLIVSGLLLAVLVQIGSAVFGTKVQLPMQQLFQDRRTALLLMLMAVLLAPVV
                     EETIFRGYLYPLIARSFGIATGVVATGIVFGLLHAPQLWGGWVQIALLVVVGIVFTYV
                     RAVTRTVVGSYLLHVSYNSFISIAFLIGTHGLRILNPGP"
     gene            1775..2827
                     /gene="mtnA"
                     /locus_tag="VN788_02580"
     CDS             1775..2827
                     /gene="mtnA"
                     /locus_tag="VN788_02580"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HXU19448.1"
                     /translation="MRTVEPIEWTSKGVVMLDQRQLPGEVVYHTYTDYRDVAKAIKDM
                     VIRGAPAIGVAAAMGVALGVQRSEANTLAEMRSQFATICDTISRTRPTAVDLFWAVKR
                     FKRRFDELASIPASDEAKALARIREVLIEEAKNLHQERREADERIGRFGAELMPKSGR
                     VMTQCNAGALATGGIGTALGVIRMAVEGGYRIEVLVPETRPYLQGSRLTAWELHQDHI
                     PLTLITDNMVGHFLKTGGVGAVVVGADRIARNGDTANKIGTYGIAVLAKENKVPFYVA
                     APISTLDLSLASGDQIPIEERSEEEVTHLAGLRISPDVRAAHPAFDVTPARYIAAIIT
                     ERGVARPPFEDSLAKL"
     gene            complement(2844..>3149)
                     /locus_tag="VN788_02585"
     CDS             complement(2844..>3149)
                     /locus_tag="VN788_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016356372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXU19449.1"
                     /translation="EINSPQPNLRNFAERTAMNTPMQGAAADLIKLAMIELDRRLTGE
                     FEARMILQVHDELLFEAPETEIPRLRNLVKEVMEGVYKLRVPLLVESKVGPNWRDMK"
BASE COUNT          627 a          966 c          864 g          692 t
ORIGIN      
        1 atcagggagt tgtatgcgat gcttccggaa tcgcgcgagc gcggctataa gcccggccgc
       61 ttcagtttca atgtgaaagg cggacgttgc gaagcgtgcc agggagatgg gcagcggcgc
      121 atcgaaatga attttcttcc cgatgtttat gtaacctgtg aagtttgccg gggcaagcgc
      181 tacaatgccg agacgttgtc cgtgcgttat aagggttgct cgatttcaga tctgctcgat
      241 ttgcccattg aggacgcgct gaaagtgctc gaaaacattc cctcgatcca gcaaaagctg
      301 caaactctgg ttgacgtcgg cctcggctac gtgcaactcg gccaatccgc caccacccta
      361 tccggcggcg aggcgcagcg caccaagctg gcgcgcgagc tttcccgcag gcagaccgga
      421 cgcactctct atatcctcga cgagcccacc accggtttgc acttcgacga tgtaagcaag
      481 cttctcaccg tcctcaatcg cctcgtcgag cttggcaaca cagtcctcgt catcgagcac
      541 aatctggacg taattaaaca agccgattgg atcatcgatc ttggtcccga gggcggacag
      601 gaaggtggac gcatcgtcgc ccagggcact cccgaacacg tagcgcgcaa taaaaaatcg
      661 tacacaggac aaatattagc ccgcgcccta aacggatcga actcgaatgg gcccggccgc
      721 tcaacgaatg ccgccgcgcg ccagctaagc gcatgatgcg cgattgtccg ccgccaatcc
      781 atctccaact atgaacttcg aagaaaacat tccgcctcct gctcagccct cggaatcttt
      841 cgatggccct tctccccagc ctgagcccgc gccgctgagc accagccagc tgccgcaaga
      901 aggcccgcca accgaatccc acattccagt ttcgccgaca ctctcccttc cggaagatat
      961 ccgcgtcccc tggggctgga ccgatctgtt gctgttcgta gtgctggcgc ttgcggcgac
     1021 atttctgttc agcatcctgc tagcaattgg ttttgcagtc ttcggcgtgc ctctttcgcg
     1081 tctccaaaag ccgggaagcg ccgaaggcct tttcgttgtg gtcagccagg caattctgtt
     1141 tttcgtgctg ctgggttatc ttttcatcca ggcgcgtgtg cgctttcatg cgcccttctg
     1201 gcgaactctc ggctggcgct ctctggaaac acatcgcatc cgtcgtggct gggcctacct
     1261 ggggctgatc gtgagtgggc tcttgctcgc ggttctcgtc caaattggtt cggccgtctt
     1321 tggcacaaaa gtgcagctac caatgcagca gttgttccag gatcgccgca ccgcactgct
     1381 tcttatgctg atggcggtct tgctggcgcc agtagttgaa gagacaattt tccgcggcta
     1441 tctttatcct ctgatcgcgc ggagcttcgg aatcgccacg ggcgtcgtcg ccacgggaat
     1501 agttttcggt ctgctgcacg cgcctcagct ttggggtggc tgggtgcaaa tcgcattgct
     1561 cgttgtcgtc ggcatcgttt tcacatatgt gcgcgccgtc acgcgaacgg tggtcggcag
     1621 ctatttgctt cacgtaagtt acaattcttt tatttctatc gcgtttctta tcgggactca
     1681 cgggctccgg atcctgaatc ctggtccgtg agccacgctg ttttcgcgac agatgtgaca
     1741 gacgcggcaa acgcactcga tcggagccaa ttcattgcgc actgttgaac ccatcgaatg
     1801 gacatcaaaa ggtgtggtca tgctcgatca gcgccaattg ccgggcgagg ttgtctatca
     1861 cacctacacc gattaccgcg acgttgccaa agccattaag gacatggtca ttcgtggagc
     1921 gcccgccata ggtgtcgccg cggcgatggg cgtggctctc ggagtgcaac gctccgaagc
     1981 aaatactctc gcagagatgc gctcgcaatt cgctacgatc tgcgacacga tttcacgcac
     2041 ccgccccacc gcagtggatt tgttttgggc tgtcaagcga ttcaagcgcc gcttcgatga
     2101 acttgcctcc attcccgcaa gcgacgaggc gaaagcgctc gctcgaatcc gcgaagtcct
     2161 catcgaagaa gcaaaaaatc ttcaccagga acgccgtgaa gcggacgaac gaattggccg
     2221 cttcggcgct gagctgatgc caaaaagcgg ccgggtgatg acgcaatgca acgctggcgc
     2281 gctagccacc ggcggaatcg gcaccgcgct aggcgtgatt cgaatggctg ttgagggcgg
     2341 ctaccgaatt gaagtgctgg ttcccgaaac aaggccgtac ctgcagggtt cgcggctcac
     2401 agcctgggaa ctgcatcagg accatatccc cctcacgctc attaccgaca acatggtggg
     2461 ccactttctg aaaacgggcg gcgtgggagc ggtggtcgtc ggcgccgacc gcatcgcgag
     2521 gaatggcgac accgccaaca aaatcggcac ctacggcatc gccgtgctcg cgaaagaaaa
     2581 caaagttccg ttctacgtgg ccgcgccgat ttcgacgctc gatctttcgc tggcctccgg
     2641 cgatcaaatt cccatcgagg agcgctccga ggaagaagtc acgcaccttg ccggcctgcg
     2701 catttcgcct gacgtgcgcg ccgcgcatcc cgcctttgat gtcactcccg cgcgttatat
     2761 tgccgcgatt atcaccgagc gcggcgtggc ccgccccccg ttcgaagatt ccctcgcaaa
     2821 attgtaggaa ttgtaagcgc ggatcatttc atgtcgcgcc agttcggccc cactttgctt
     2881 tccaccagca aaggaacgcg caatttatat acgccttcca tcacttcttt caccagattc
     2941 ctcagccgcg gaatttccgt ctcgggcgct tcaaacagca gttcgtcgtg cacctgaaga
     3001 atcatccggg cctcgaactc tccggtcaaa cggcgatcga gctcgatcat ggccagcttg
     3061 atcaggtcgg ccgccgcgcc ctgcatcggc gtgttcattg ccgtgcgctc ggcgaagttg
     3121 cgcaagttcg gctgcggcga attgatttc
//
LOCUS       DATNDT010000087         4047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1773984, whole genome shotgun sequence.
ACCESSION   DATNDT010000087 DATNDT010000000
VERSION     DATNDT010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1773984"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..785
                     /locus_tag="VN788_02590"
     CDS             <1..785
                     /locus_tag="VN788_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXU19450.1"
                     /translation="TSAGLIQLFEARWSPDQLQLHLEAGRRVDAIRAAAFAKVRENLA
                     AGMAIGELYVKRFILESFERVGLVADHGPIVAVNRNMSNPHYEPMDGRSEAIRRGDGL
                     LIDMWAKLNEPDAVYYDITWTGFCGTEPPAELVRIFDAVRGARDAAIGRVKLATETGE
                     DLRGFQVDDAARSYIDGQGFGEYFVHRTGHSIGEEVHGTGANMDNLETHDDRKVIPGT
                     GFSIEPGIYLPDFGVRSEVNMYVGERGAMVTGEIQQELLRIA"
     gene            778..2328
                     /locus_tag="VN788_02595"
     CDS             778..2328
                     /locus_tag="VN788_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU19451.1"
                     /translation="MHSSKTKYFAIIIFLACALTSQAADWPTFAGNPQRTGWAKDETA
                     VNKDNVSKMKLEWSLKVDNTALELNALTVPVIIENVITPGAFRDLVVVGGSSDNLSAI
                     DADTGKLVWHKQFQVEGKSKQQANWLCPNALNAAPVIDLASHTVYTVTSDGILHSLNV
                     VNGEDRKPPIRFVPSYAKTWSLNLYKDVLFTATSQGCNGVRSLVYAMKLNDPNRAISS
                     FNAGPTGGAGIWGRAGVAISDVTALAYAEAGDGPWNPVEGKYSNSFLAVSADGKLADY
                     YTPTDYAWISKKDLDMGNISPVVFPFDKWELVAGGGKQGVLYLLDAKSLGGVDHRTPL
                     YRSPLLLNDGVDFAGHGFWGALSSWKDEMGTTWLYAPAWGPVATEAPKFPVSYGTVTA
                     GSIMAFKLSVKDGKPVPEAAWISRNLSYPEPVVIANGIVFAVSNGENVKQVDSAGDIL
                     SSAQRANAPTGNATLYAFDAETGKELFSSGKTIPGFTHFSGLAISSGRVYVVTHDSIV
                     YAFGLGRK"
     gene            2401..3159
                     /locus_tag="VN788_02600"
     CDS             2401..3159
                     /locus_tag="VN788_02600"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXU19452.1"
                     /translation="MSVTEPDSSQVEILHRISSIVSSELSLDEMLGEIVGLTVQVTGC
                     DACLVYLIERETGEIVLRASQVPHAADLGHIRLKMGEGVTGWVAEHKSVVALASNAFA
                     DSRFKPFERLIEDTYQAFLSVPLVSGGDLIGVINVHHRQRHDHTAGEIALLTFVGEQM
                     GGAISKSLLADENARLLEETQEMKRQLEIRKLVERAKGILQHRHNLTEEEAYLRLRNE
                     SRRLRRPMKDLAEAIILAEDINRKPDSADGSQLG"
     gene            3197..>4047
                     /locus_tag="VN788_02605"
     CDS             3197..>4047
                     /locus_tag="VN788_02605"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXU19453.1"
                     /translation="MRPFIPLFRNAHLLTVAGNFWPRPLDIVRFPVRDRYYAVEPGVE
                     ILVHEQQPQGTPRGQIVLVHGLEGGSDSGYMRSLAQAALERGFGVHRFHMRSCGGTES
                     RSGNVLYHSGQTGDLKFVTCTIAEERGGSLFLTGFSLGGNVVLKLAGELGEDAATWIA
                     GVCAVSTPIDLGICARSLEKPSNFLYENRFLGRLKDRIRRKAALMPGLFCLEDLPRAK
                     TVRDFDDLFTARCFGFGTAEHYYESQSSGQYLERIRIPALLVQAKDDPLIPFEIYNNH
                     PAFRRNP"
BASE COUNT          866 a         1062 c         1235 g          884 t
ORIGIN      
        1 caacgtcggc ggggctgatt cagttgttcg aagcgaggtg gagtccggat cagttgcagt
       61 tgcatctcga agcgggccgg cgcgtcgatg cgatccgcgc ggcggcgttc gcgaaggtgc
      121 gcgaaaacct cgccgcagga atggcgattg gcgagttata tgtcaagaga ttcattctcg
      181 aatcgtttga acgcgtcggg ttggtggcgg atcatggtcc gatcgtggcg gtaaatcgta
      241 atatgtcgaa tccgcactac gaaccgatgg atgggcggag cgaggcgatt cgcagaggcg
      301 acggtctgct gatcgacatg tgggcgaaac tgaacgagcc agacgcagtg tactacgaca
      361 tcacgtggac cggattttgc gggacagaac cgcctgcgga actggtgcgc atctttgatg
      421 ccgtgcgagg cgcgcgcgat gcggcaattg gcagagtgaa gctagccacg gaaaccggtg
      481 aggatcttcg cggatttcag gtggacgacg ccgcgcgatc gtatatcgat ggccaggggt
      541 tcggcgaata tttcgtccat cgcaccggtc attccatagg agaggaagtg cacggtaccg
      601 gcgctaacat ggacaatctg gagacccacg acgaccgcaa ggttatccct ggaacgggtt
      661 tttcgattga acctggcatc tatcttcccg actttggcgt ccgctcggaa gtaaatatgt
      721 atgttggtga gaggggcgcg atggtgacag gagaaattca gcaggagtta cttcgcattg
      781 catagctcaa aaacgaaata cttcgcaatt atcatttttt tagcttgcgc attgacctcg
      841 caggcggccg attggccgac gtttgccggt aaccctcagc ggactggctg ggcgaaagac
      901 gagacggcgg ttaataaaga caacgtctcg aagatgaagc tcgagtggag tctgaaggtc
      961 gacaacacgg cgctagaatt gaacgctctt actgtgccgg tgattattga aaatgtcatc
     1021 acccctggag catttagaga ccttgtcgtg gttgggggaa gttcggacaa tttgtccgcc
     1081 atcgacgccg acacggggaa gctggtttgg cacaagcagt ttcaggtgga gggtaagtcg
     1141 aagcagcaag cgaactggct ttgcccgaac gctctgaacg ccgcccccgt gatcgatctg
     1201 gcgtcgcata ccgtctacac cgtcacgagc gacggaatcc ttcattcgct caatgtggtc
     1261 aatggcgaag atcggaaacc cccgatacga ttcgtgcctt cctatgcgaa aacatggagc
     1321 cttaatctct acaaggacgt gctattcacg gcaacgtctc agggctgcaa cggcgtgcgc
     1381 tccttggtgt atgcgatgaa attgaacgat ccgaaccgcg cgatttcttc attcaatgcc
     1441 ggccccacag gtggagcggg aatctgggga cgcgccgggg tcgcaatcag cgacgtgacg
     1501 gcgcttgcgt acgcggaggc cggcgatggc ccgtggaatc ccgtggaagg aaaatactcg
     1561 aacagctttc tcgcggtttc ggccgacggc aagctggccg attattacac gcccaccgat
     1621 tacgcctgga tttcaaaaaa ggatcttgac atgggcaaca tcagtccggt cgtgtttccg
     1681 ttcgataaat gggaactcgt cgctggcggg ggcaagcagg gagtgcttta tcttttggac
     1741 gccaaatcgc tcggaggcgt agaccatcgt acgccgcttt atcgcagccc tctgcttctg
     1801 aacgacggcg tcgatttcgc gggacatggt ttctggggtg cgctctcctc gtggaaggac
     1861 gaaatgggga cgacatggct ctacgcgccg gcgtggggtc cggttgcgac agaagctccg
     1921 aagtttccgg tcagttatgg cactgtgacc gccggcagca tcatggcgtt caagctctcc
     1981 gtgaaggacg gcaagcctgt accggaagcg gcctggatct caaggaatct ctcctatccg
     2041 gagccggtcg tcattgcaaa tgggatcgtg ttcgcggttt cgaatggcga gaatgtgaag
     2101 caagtagact cagctggaga cattctgtcg tccgctcagc gcgcgaacgc tccgactgga
     2161 aacgcgacgt tgtacgcatt cgacgcggaa acgggcaagg agcttttttc gagcgggaag
     2221 acgattccgg gcttcactca tttcagcggc ctcgcgatct ctagcgggcg cgtgtatgtc
     2281 gttacgcacg atagcatagt gtatgcgttc gggttaggcc gcaagtaggc ggataccctc
     2341 aatcgaaagt ttcgattgga ctgaagcggt ccgccacggg taccctgaaa tatggcaatc
     2401 atgtctgtta cggagcccga ttcctcgcag gttgaaattc ttcatcgcat cagcagtatc
     2461 gtgagttcgg aactgtcgct cgacgagatg ctgggagaga ttgtcggtct aacggtacaa
     2521 gtcactggct gcgatgcctg tctagtctac ttgatcgaaa gggagaccgg tgagattgtg
     2581 ctgcgggcct cgcaggtacc gcacgcagcc gatcttgggc atatccggct caagatgggc
     2641 gagggcgtta cgggctgggt agcggagcat aaatcggttg tcgcgctcgc ttcgaatgcg
     2701 ttcgccgact cccgtttcaa accgtttgag cgcctgatcg aagataccta tcaagctttt
     2761 ctttcggtgc ccctggtcag cggtggcgac ctgattggtg tgatcaacgt acaccatcgc
     2821 cagcggcacg atcacacggc cggcgagatc gcattgctga ctttcgtcgg cgagcagatg
     2881 ggcggagcaa tttcaaaatc actgcttgcc gacgagaacg cgcgacttct tgaagagacg
     2941 caagaaatga agcgccagct cgaaattcgt aagctggtgg aacgcgcgaa gggaattctc
     3001 cagcaccggc acaatctgac ggaagaagaa gcctatttgc gcttgcggaa tgagagccgc
     3061 cgtttacgca ggcccatgaa ggatctggcg gaagcgatca ttttggccga ggatatcaac
     3121 cgtaagccgg attcagcaga cggatcgcag ctgggctgat cgcaggcgcg gttcgaaaca
     3181 acgctaaaat agcgctttgc ggcctttcat tcctctattc cgaaacgcgc acctgcttac
     3241 cgttgcgggc aacttctggc cgcgtcctct cgatatcgtt cgttttcccg tgcgggatcg
     3301 ttattacgcc gttgaaccgg gcgtcgaaat tctagtgcat gagcagcaac cccaaggcac
     3361 gcctcgcgga cagatcgtac tggtgcatgg ccttgaagga ggcagcgatt cgggttatat
     3421 gcgcagtttg gcgcaggcgg cgctggaacg tggattcggc gtccatcgct ttcacatgcg
     3481 cagttgcgga ggcacggaat ctcgcagcgg caacgtgctt tatcactccg gccagaccgg
     3541 tgacttgaag tttgtgacct gcacgattgc ggaggaacgg ggtggctctt tgttcctgac
     3601 cgggttctcg ctcggcggca acgtggtttt gaaactcgcg ggtgaacttg gcgaggatgc
     3661 ggcgacgtgg atcgccggcg tctgcgcggt ctccacgccc atagatctgg gcatctgcgc
     3721 ccgatctttg gaaaagccat ccaacttcct ttacgaaaac cgttttctcg gtcgcctgaa
     3781 agaccgcatc cgccgcaagg ctgctctcat gccaggattg ttctgcctcg aggatctgcc
     3841 cagagcgaag accgttcgcg atttcgacga cctcttcacc gcgcgttgct tcggctttgg
     3901 cacggcggag cactattacg agagccaatc ctccgggcaa tatctggaac ggattcgaat
     3961 tcccgcgttg ctggtgcaag ccaaggacga tccattgata ccgttcgaga tttacaacaa
     4021 tcatcccgct ttccggcgaa atccaaa
//
LOCUS       DATNDT010000088         7904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1490575, whole genome shotgun sequence.
ACCESSION   DATNDT010000088 DATNDT010000000
VERSION     DATNDT010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7904
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490575"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..316
                     /locus_tag="VN788_02610"
     CDS             <1..316
                     /locus_tag="VN788_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19454.1"
                     /translation="LPLNPSPPPPNFEQRSNQLRTLFGYGDQNRPGLNLMMGASYDFT
                     RVAFQNQIVEVSYNGLCCGIGFEYRRFSFGAIRNENQYSVVFRIANLGSAGTLRKQEK
                     IF"
     gene            326..1738
                     /locus_tag="VN788_02615"
     CDS             326..1738
                     /locus_tag="VN788_02615"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXU19455.1"
                     /translation="MTLKSESELAFFSIEEAASLLRRKELSPVDLVNASLARIERWNP
                     DMHAFLTILAEPARRRAKLAEKEILRGSDRGPLHGIPISLKDNYYTRGIRTTAGSKIL
                     ADFVPETDSHVAARLARAGAILLGKTNLHEFAYGITGENPHYGSSRNPWAPERITGGS
                     SGGSAVAVATGMGFASMGTDTGGSIRVPSALCGIVGLKPTFGLVDISGVVPLAVSFDH
                     AGPIARSVTDACIVLESIAGKYPKGAARPDHRKLHENLPARFRLGWPKDFFFDRLDDE
                     VRRLIAATAKVFEQLGAEIQEVSLPHLSNSVDQATSAVVAEASHYHESQGYFPARAAE
                     YGEDVRGHLEWGHRLLAVDYLRAVDARREVEQDFASAFENVDAIIAPTSPITAPLIGE
                     SEVRVAGQRESAVRAELLRLTRPANLTGSPAISLPCGFTPQGLPVGLQLIAPRWSEAR
                     LLAIALAYEQSTDWHLRHPM"
     gene            complement(2013..2429)
                     /locus_tag="VN788_02620"
     CDS             complement(2013..2429)
                     /locus_tag="VN788_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXU19456.1"
                     /translation="MKYLLDVNTLLALVVVEHEFHARAASWVERLGDSGTSELATCSI
                     TELGFVRVLGQVQQYGSSVAQARELLLKVKNSDGMRWIFIPDDRDIAHLPKWVRTPKQ
                     ITDGHLAELAKANEAVLATLDRQIPGAFVVPQSRHS"
     gene            complement(2426..2683)
                     /locus_tag="VN788_02625"
     CDS             complement(2426..2683)
                     /locus_tag="VN788_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXU19457.1"
                     /translation="MRTTISTKGQVVVPSQIRRKLGLQPGDSLEATVEGEKIVLTPRR
                     IRSRKARIIRDPVTGLPVLTAGPKAPKLMSAEVREILAEFP"
     gene            2905..3771
                     /locus_tag="VN788_02630"
     CDS             2905..3771
                     /locus_tag="VN788_02630"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HXU19458.1"
                     /translation="MEVSIFGVFLAGVISFLSPCVLPLVPGYVSMLSGVGVEQLKEGE
                     GGTGRLLTLAFAFVIGFSVVFITLGASASAVGSFMLRNRTLIAPIAGALIILFGLHLV
                     GWLIKIPVKIGIIVGVVLVAIGLALNLSYGNRFEIKPVQFYAASLIFLLGPGMTRWLN
                     RDVHLRNVGGGSQPGIVSGFLLGFAFAFGWTPCIGPILTSVLALAATKDTIGQGVLLL
                     AFYSAGLAIPFLLTAVGIGRFLKFYKRFRKHLHTVEVFSGVLLLAIGGLIFFNELTLL
                     SQHLTFLNRFVK"
     gene            3785..4348
                     /locus_tag="VN788_02635"
     CDS             3785..4348
                     /locus_tag="VN788_02635"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXU19459.1"
                     /translation="MNRVYSAFLSAIVILATAVSFSGCMSSSKSSSSSSRTTVAPPLA
                     NEPDVTFKDLQGNNVTLSSFKGKVVLVNFWGTWCEPCRGEIPILIKMQEQYGSKGFTV
                     LGVATNDDIKAVDPFIHHTQFNVDGHEMTMNYPIVMGSDEISTKFGGLLGMPTSFLIS
                     RDGKIIKRYIGAFANGDEINKQVESHL"
     gene            4345..4920
                     /locus_tag="VN788_02640"
     CDS             4345..4920
                     /locus_tag="VN788_02640"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HXU19460.1"
                     /translation="MSLSKSSRYLAMLSALLSIGAIAMAQDASSPKRAADVVKAHPYV
                     SLDPVPRGKTFEAAVVVQIAEGYHMNSHKPSDAYLIPTTLTPKLPEGIHLVDTIYPPG
                     RLKKFAFSPDKPLDVYSGSVTLRLKLTAQDSAAIGATTIPMVLRYQACNNSACLPPVK
                     IPVTAELKIAAAGATAKPVHSDVFAAPKPSN"
     gene            complement(4996..6480)
                     /gene="gltX"
                     /locus_tag="VN788_02645"
     CDS             complement(4996..6480)
                     /gene="gltX"
                     /locus_tag="VN788_02645"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXU19461.1"
                     /translation="MPQSQKPRVRFAPSPTGYLHVGGARTALFNWLYAKREGGTLLLR
                     IEDTDVERNRPELVEGILDGLKWLGIEWDGEPIYQSQRLPLYRAAAERLLASGAAFAC
                     FCKAAAYAGGDAATSEEEREGEGDDEGAKTYKAIPCPCRDLTESERTEKERAGAARAI
                     RFRVAREGVTKFEDAVFGPREVQNSEIEDFVLLRSNGVPTYQLSVVVDDIDMQISHII
                     RGADHLSNTPKQALIYQALGATPPVFAHVPLILGPDRTRLSKRHGATSVGSYAEEGFL
                     PEAFRNFLALLGWSPGDDSEFLRTKELIERFALSGVSRTNAVFDRAKLEWFNTQYLQK
                     PPVEELLPYVEAELKRAGLWEDSQASRDPAWFARTVDLIRPRTRLLGDFTTWARAFFT
                     DDFEYETEARQKFWKDERLPAMLAKLADALAGVADWNHDACDAALRALATAEGVKAGL
                     LINATRVAIVGRAVAPPLFETMVVLGKERVVARLRRALPAVSAR"
     gene            complement(6644..6898)
                     /locus_tag="VN788_02650"
     CDS             complement(6644..6898)
                     /locus_tag="VN788_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19462.1"
                     /translation="MNGKPNVRFSELCSKYSSFWQSLLRFGLPFILIYYLVNYIIFRV
                     TLKNTGLQYPWKSAVIPDVFFMFLVSAVWWIVMRQFAGRK"
     gene            7106..7735
                     /locus_tag="VN788_02655"
     CDS             7106..7735
                     /locus_tag="VN788_02655"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXU19463.1"
                     /translation="MMNRLERRYGLGHLHFITCSCYRRKPLLGTERARSLFLKILAEV
                     RERYDFALIGYVVMPEHIHLLMGEPRRGTPTTVMQVLKQRVSRALHGRRPRKGRSPGQ
                     MRLWDEASAKKRNPFWQRRFYDFNVWSWKKKNEKLNYMHFNPVKRGLVRHPKEWLWSS
                     YAFYWKGITNACPPNPDWRPGSEAGGGLKRKTHPSETRRVRHPLSFFEA"
BASE COUNT         1692 a         2297 c         2179 g         1736 t
ORIGIN      
        1 cttgccgtta aacccctcgc cgcctccgcc gaatttcgag cagcgctcca atcagttgcg
       61 cacgctcttc ggttacggcg atcaaaaccg cccgggcttg aacctgatga tgggagccag
      121 ttacgatttc acgcgagtgg cctttcagaa ccaaatcgtc gaggtctctt ataatggctt
      181 gtgctgcggt atcggttttg agtaccgccg cttttcgttt ggcgcgattc gtaatgaaaa
      241 tcaatacagc gttgtgtttc ggatcgccaa tctcggctcg gctggcactc tgcgaaagca
      301 agagaaaatc ttctagcgag attacatgac cttaaaatcc gaatccgagc tcgcgttttt
      361 ctcaattgaa gaagcggcct cgttactgag acgcaaggag ctttcccccg tcgatctcgt
      421 caacgcatcg ctcgcgcgca tcgagcgatg gaatcccgat atgcacgcct tcctcaccat
      481 cctcgcggaa ccggcgcgcc gccgcgcgaa gctggccgaa aaagaaattc tccgcggctc
      541 ggaccgcggc ccgctccatg gcatacccat ttcgctgaag gacaactatt acacgcgcgg
      601 cattcgcacc accgccggct ccaaaattct cgctgatttc gtccccgaaa ccgatagcca
      661 cgtcgctgcc cgtctggcgc gtgcgggcgc catcttgttg ggcaaaacca acctgcatga
      721 atttgcctac ggtatcaccg gcgaaaatcc gcattacggc agctcgcgca atccttgggc
      781 tcccgagcga attaccggcg gatcaagcgg tggttcggct gtcgccgtgg ccaccgggat
      841 gggattcgcc tccatgggaa cggataccgg aggctccata cgcgtcccat ccgctttgtg
      901 cggaatcgtc ggattgaagc ccacattcgg actggtggac atttctggcg tcgtgcccct
      961 cgcggtcagc ttcgatcacg cgggaccgat cgcgcgcagc gtaaccgacg cgtgcatcgt
     1021 cctcgaatcc atcgcgggaa aatatcccaa gggcgcggcg cgtcccgatc accgaaagct
     1081 ccacgagaac ctgcccgcgc gattccgcct cggctggcca aaggattttt tctttgacag
     1141 gctcgacgat gaagtgcggc gcctgatcgc agccaccgca aaagtcttcg agcaattggg
     1201 cgccgaaatc caggaggtgt cgctacctca tctgtcgaac tctgtcgatc aagcgaccag
     1261 cgccgttgtc gctgaagcga gccactatca cgaatcgcag ggatatttcc ccgcgcgcgc
     1321 cgccgagtat ggcgaagacg tccgcgggca cctcgagtgg ggtcatagac tgctcgccgt
     1381 ggattatctt cgcgccgtgg acgcaagacg tgaggtcgaa caggatttcg cgtccgcttt
     1441 cgaaaacgtg gacgccatca tcgctcccac ttcccccatc actgccccgc tgatcggcga
     1501 aagcgaagtc cgtgtcgcgg gccagcggga atcggccgtt cgagcggaat tgttgcggct
     1561 cactcgcccc gccaatctca ccggctctcc ggcaatctcg ctcccctgcg gattcacgcc
     1621 gcaaggtttg cccgtaggtc tgcagctaat cgccccgcgc tggagcgaag cccgtctgct
     1681 tgccatcgcc ctcgcctacg aacaatccac cgattggcac ctccgccacc ccatgtagct
     1741 caccccgctt tcacggggtg aggctttcgc acccatggtg cgatccaacg cccatcctcg
     1801 cgtcgtcatc cctcgcgcag cgaagaatcc ctcttgatag gggcacgata tatcgtgccc
     1861 ggcgcacacg cctttgcctt ggtttcgtag gggcatagca cgctatgccc tccgcagatg
     1921 cttggctaca tgcaagccat ctatctcaac acggggagcg gtttcgctct tgcatttacc
     1981 cgctgaaaac taaaacctgt agcatggcct cttcacgaat ggcggctttg ggggacgacg
     2041 aacgcaccgg ggatttggcg gtccagcgtg gccaaaacgg cctcgttggc cttggcgagc
     2101 tccgcaaggt gcccgtcggt gatttgcttt ggagttcgga cccacttggg gagatgggcg
     2161 atgtcgcggt catcgggaat gaagatccag cgcattccat cgctattctt caccttgagc
     2221 aagagctcac gcgcctgcgc gactgacgac ccgtactgtt gaacctgtcc taggacgcgc
     2281 acaaagccca attcggtgat cgagcatgta gccagttccg aagttccgct atcgccaagg
     2341 cgttccaccc agcttgcagc tcgcgcatga aactcatgct ccactacgac gagcgcaagc
     2401 agagtgttca catccaatag gtatttcatg gaaactcggc gagaatttcg cgcacctctg
     2461 cgctcatcag cttaggagcc ttcggaccgg cggttagaac cggcaatccc gtgaccggat
     2521 cccgaatgat gcgtgccttt cgcgaacgaa ttcttcgcgg ggtcagcaca atcttttccc
     2581 cctcgactgt ggcctccaac gagtctcccg gttgcagtcc gagcttgcgc cggatctggc
     2641 tggggacgac aacttggccc tttgtcgaaa tcgttgtacg catggtaaga ccatcttacc
     2701 agaaataggg cgcgcaagaa ggcaggaatg ctaagctaat cgaaaggtca tcgcttccaa
     2761 aatccttccg cttttacgcc ctccaaagcc ctgaaccaaa aatggatagt aagcgtctaa
     2821 tggatatggg aaaatgcgcg tttgtgcgca gcccgacgct acgccccgat ggctcggaag
     2881 cgtcgaaaat aggaaggtaa ccggatggaa gtttccatct ttggcgtatt tctcgcgggc
     2941 gtgatctcgt tcctttcccc gtgcgtcttg ccccttgtgc ccggatacgt ttcgatgctt
     3001 tcgggcgtgg gtgtagagca attgaaggaa ggcgaaggag gcacaggcag gcttctgacg
     3061 ttggccttcg cattcgtgat tggtttttcg gtcgtcttta tcaccctggg cgcttcggcc
     3121 agcgcggtgg gctcgttcat gttgcgtaat cgcaccttga tcgcgcctat cgcgggtgca
     3181 ttgatcatcc ttttcgggct gcacctggtc ggatggctga tcaagattcc ggtcaagatc
     3241 ggaatcatcg tgggcgtcgt gctcgtcgcc atcggtcttg ccctcaacct gagttatgga
     3301 aacagatttg agattaagcc ggtgcagttt tacgccgcgt cgctcatctt cctgctggga
     3361 cccgggatga cgcgctggct caaccgggac gttcatctgc gcaatgtcgg tggaggcagc
     3421 cagcccggaa ttgtgagcgg attcctgttg ggcttcgcct ttgccttcgg atggacgccg
     3481 tgcatcggtc cgatcctcac cagcgtgctc gcgcttgcgg ctaccaagga caccatcggc
     3541 cagggcgttc tgttgctggc gttctattcg gcgggactgg cgattccatt tttgctcacg
     3601 gcagtgggca tcggacgctt tttgaaattt tacaagcgtt tccgaaaaca ccttcacacg
     3661 gtggaggttt tcagcggagt gcttctgctt gccatcggcg gcttgatttt cttcaatgaa
     3721 ttgacgttgc tttcgcaaca tttgacgttc ctaaatcgat ttgtgaaata aaatggagtc
     3781 gaggatgaat cgggtctaca gcgcatttct atcggcaatc gtaatcttag cgactgcggt
     3841 gagtttttcc gggtgcatga gcagcagcaa atcgtccagc agttcttcta gaacgaccgt
     3901 ggccccgccc ctggccaacg agccggacgt taccttcaag gatttgcaag gcaataacgt
     3961 tacgctctcc agcttcaagg ggaaggttgt tctcgtgaat ttctggggca cctggtgcga
     4021 gccttgccgc ggcgaaattc ctatactcat caaaatgcag gaacagtacg gcagcaaagg
     4081 cttcacggtc ctcggcgtcg ccaccaatga cgacataaaa gccgtcgatc cgttcataca
     4141 ccacacacag ttcaacgtcg atggtcatga gatgaccatg aactatccga ttgtcatggg
     4201 aagcgatgag atttcaacaa agttcggcgg cctgctcgga atgccgacga gcttcctgat
     4261 ttcgcgcgat ggtaaaatca tcaagagata catcggcgcg ttcgcgaacg gcgacgaaat
     4321 caataagcaa gtggagagtc acttatgagc ctttccaaaa gttcccggta cctcgcaatg
     4381 ctgtccgctt tgctttcaat tggcgcgatt gccatggcgc aagacgcttc aagtccgaag
     4441 agagccgccg atgttgtgaa agcccacccg tatgtttcgc tggatcctgt tccgcgcggc
     4501 aagacctttg aggctgccgt agtcgtccaa atcgctgagg gataccacat gaattcgcac
     4561 aagccctcgg acgcctactt gatcccaact actctcactc cgaagttgcc ggagggcatc
     4621 catttggtgg acacaatcta tccgccgggc cgcctcaaaa agtttgcttt ttctccggat
     4681 aagcccctgg atgtttacag cggaagcgtg accctgcgtc tgaaactcac tgcgcaagat
     4741 agtgctgcaa tcggagccac cacgataccg atggtcttgc gctatcaagc gtgcaacaac
     4801 tcggcctgtc tgcctccggt taaaattccc gtaaccgccg agctgaaaat tgccgccgcg
     4861 ggcgccaccg caaaaccggt ccattccgac gtttttgccg cgccaaaacc ttctaattga
     4921 aagcctggaa agattctagt tcgaagcatc caaatcatct aactagaaac aataagcggt
     4981 cccaaaacgg caaattcagc gcgctgagac cgctggcaag gcccgcctga gccgcgccac
     5041 cacgcgttcc tttcccagca cgaccatagt ctcaaaaagc ggcggagcaa ccgcccgccc
     5101 cacaatcgcg acgcgcgtcg cgttgatcag caacccggct ttgacgcctt cggcagtcgc
     5161 gagggcgcgc aaggccgcgt cgcaagcgtc gtggttccaa tccgcgactc ccgcgagcgc
     5221 gtctgcgagc ttcgcgagca ttgccggcaa ccgctcgtct ttccagaatt tctgtcgcgc
     5281 ctcggtttca tactcgaaat cgtcggtgaa aaacgcgcgc gcccacgtcg tgaaatcgcc
     5341 aagcaagcgc gttcgaggac gaatcaaatc caccgtacgc gcgaaccacg ccgggtcgcg
     5401 gcttgcttgc gaatcttccc aaagccccgc gcgtttgagc tccgcttcca cgtatggaag
     5461 cagctcttcc acgggcggct tctggagata ctgcgtgttg aaccattcga gtttcgcgcg
     5521 atcgaagacg gcgttggtgc ggctcacgcc cgataacgcg aagcgctcga tcaattcttt
     5581 tgtgcgcaga aattccgaat catctcccgg tgaccagccc agcagtgcga gaaaattccg
     5641 aaacgcctcc ggcaaaaatc cctcttcagc gtacgagccg acgctcgtgg ctccgtggcg
     5701 tttggaaagc cgcgtgcgat cgggaccgag aatcaatggc acgtgggcaa acaccggcgg
     5761 ggttgcgccg agcgcctgat aaattagggc ctgtttcgga gtgttggaaa ggtgatcggc
     5821 gccgcgaatg atatggctga tttgcatatc gatgtcgtcc accaccacgc tcagctgata
     5881 ggtgggaact ccgttggagc gcaacagcac gaaatcttct atttccgaat tctgcacttc
     5941 gcgcggacca aaaacagcgt cttcgaattt cgtaacgccc tcgcgcgcca cacggaagcg
     6001 gatcgcgcgc gctgctccgg cgcgttcctt ctcggtgcgt tcagattccg tgagatcgcg
     6061 acacggacac ggaatcgcct tataagtttt tgcgccttcg tcgtcgcctt caccttcgcg
     6121 ctcttcctca ctagtcgcag cgtcaccgcc ggcgtaagcc gcagctttac aaaaacacgc
     6181 gaaagccgct ccgctggcga gcaggcgttc cgctgcagcg cggtaaagcg gaaggcgctg
     6241 cgactggtaa attggttctc catcccattc aattcccagc cacttcaatc cgtcgagaat
     6301 tccttccact aactcaggac gattgcgctc aacgtccgta tcttcaattc gcagaagaag
     6361 cgtgcctccc tcgcgcttgg cgtagagcca gttgaagagc gcggtgcggg ctcctcccac
     6421 gtgaagatat ccagttgggg aaggcgcaaa gcgaacgcga ggtttctggg attgaggcat
     6481 gccttgtttt cggtagatcc gaagcgattc atcttaacgg aacgtcacgg caaaatcgag
     6541 ggactcaggc ctggtaactt cggtggactg agatcagcga cccgcgtcat tcgcaaggag
     6601 ggtgaagctt cctacatcgt gaaaattggc ttgcggcagc agctcacttg cgaccggcaa
     6661 actggcgcat aacaatccac cagacagcag aaacgagaaa cataaagaag acgtctggaa
     6721 tgactgcgga tttccaaggg tattgaagcc cggtgttctt aagggtcacg cggaaaatta
     6781 tgtagttaac taaatagtaa attaggataa aaggaaggcc aaaccgcaaa agcgattgcc
     6841 aaaaactgct atactttgag caaagttcac tgaagcgcac gttcggtttt ccattcatga
     6901 tcgatctcgt cctcgtttca ctgagagcac gcggcgaggt ccttcttcag ttggcgggtg
     6961 gcgcaccctt tctctccgta ttttccacca ttgacatcaa cgcgcgggcg gatgatacca
     7021 caccagaatt aagctagaga ttcgatccaa cttcgtgggt gccgcactct tcgcggcgtt
     7081 tgcgaagggt gcgggggtgt ttcaaatgat gaaccgactc gaacgccggt acgggctggg
     7141 tcacctgcac ttcatcactt gcagttgcta tcggcgcaag cctttgctgg gaaccgagcg
     7201 ggcgcgcagt ctttttctta agattctggc tgaggttcgg gaacgctatg actttgcgtt
     7261 gatcggttat gtggtgatgc cggagcacat tcatttgctg atgggagagc cgagaagagg
     7321 aactccgacg acggtgatgc aggttttgaa gcagcgagtt tcgcgagccc tgcacggtcg
     7381 gcggccgaga aagggcagat cgcccggaca aatgcgttta tgggacgaag cgtccgcgaa
     7441 gaaacgcaac ccattctggc agcgacggtt ctacgacttc aacgtgtgga gctggaagaa
     7501 aaagaacgag aagctgaatt acatgcattt caatccggtg aaacgagggc tggttagaca
     7561 tcccaaggag tggttatgga gtagctacgc gttttattgg aaagggatca cgaatgcgtg
     7621 tccgccgaat cccgattgga ggccgggcag cgaggccgga ggaggactga aaagaaagac
     7681 gcacccttct gaaacgcgaa gagtgcggca cccactaagt ttttttgagg cttgagggca
     7741 gatcgggtgt gactgcgcac gcggaaatga attggaaagg gtgcgccacc ctctatgacg
     7801 ttgtcggctc tgtcaagaga cacctacagt ttgcgaacca cggatggttc gttttcttct
     7861 ttcctaagca ggtcctttct ctgatcgcga ccaaggctcg cacc
//
LOCUS       DATNDT010000089         4125 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2347315, whole genome shotgun sequence.
ACCESSION   DATNDT010000089 DATNDT010000000
VERSION     DATNDT010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2347315"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1558
                     /gene="trpE"
                     /locus_tag="VN788_02660"
     CDS             77..1558
                     /gene="trpE"
                     /locus_tag="VN788_02660"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HXU19464.1"
                     /translation="MIQPDFKTFRCLAQQGNLIPVYETYTADLLTPVGAYLRITRDSK
                     YSFLLESVEGGETIGRYTFAGVNPEEVFRARGRHCTLEKGGKKVHFVENPIEQLRRLT
                     AQYRTVRVPGLPPLIAGAIGYFSYDMVRLVERIPDTGRDDVAADDCVMMFYLGLLAFD
                     HVKHRVWVIRNVFIEGPGSLRAKYDAAIREIRHTRGLLEKPLAPRRHARRGSSLRVRS
                     NMTKSAFTNAVGRAKEYIRAGDAFQIVVSQRFEARTKANPLDIYRALRVVNPSPYLYY
                     LQLGDMSVVGSSPEMLVKVQGRDAFYRPLAGTQPRGRNEMEDRELEEKLLADPKERAE
                     HVMLVDLGRNDLGRVCEYGSVKAERLMFVERYSHVMHLASSLRGRLRGDVDCFDALMA
                     CFPAGTLSGAPKIRAMQIIDELEPTRRGVYGGAILYLDFSGNLDSCIGLRTLIAKNGR
                     AYVQAGAGIVADSVPEREYEESGDKARAVLKALEIAHRGGSKE"
     gene            1610..2173
                     /locus_tag="VN788_02665"
     CDS             1610..2173
                     /locus_tag="VN788_02665"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HXU19465.1"
                     /translation="MLLLLDNYDSFTYNLAQYLGQMGQALEVRRNDQITLDEIEYLQP
                     ERIVISPGPCTPAEAGISVPLIECFAGKIPILGVCLGHQAIGAAFGGRVILADCVMHG
                     KTSEIHHDGKTIFRDLPQNFPATRYHSLIVERKSLPPTLEISAETADGTIMGLRHRKL
                     KVEGVQFHPESVLTEAGFQLLQNFLSI"
     gene            complement(2230..3291)
                     /gene="trpD"
                     /locus_tag="VN788_02670"
     CDS             complement(2230..3291)
                     /gene="trpD"
                     /locus_tag="VN788_02670"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HXU19466.1"
                     /translation="MLHEAVLKVDAKTDLSRSEAEAAMEEILSGRATNAHIIGLLKGL
                     QTKTETLDEIVGFAKAMRRHATPIFRPNHPAADKALIDTCGTGGDGCGTFNVSTVAAF
                     VVAGAGVRVAKHGNRSISSRCGSADVLEQLGGRIDLTPGSVARCIESVGIGFLFAPAV
                     HAATRFAMPARRRLNGRTVFNLLGPLTNPAGASAQLVGVYDKGVIELMARALGELGVR
                     RAFVVHGDDGLDEISISGVTHVAELQDGRVRSFTVTPADFGLQGAPPEAIRGGDSKQN
                     AQIVHKILGRSMLYRDHGPHREIVLANAAAALVAGGRAENFLEGARLAAQSIDSGAAR
                     ERLDAFIAFSQIESGPVFP"
     gene            3401..>4125
                     /locus_tag="VN788_02675"
     CDS             3401..>4125
                     /locus_tag="VN788_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HXU19467.1"
                     /translation="MKIHEYQAKAILAKFGVSVPRGRVVRKKDKARAAAEELGLPVVV
                     KAQIHAGGRGKGGGVKVVKSADEAASFAEHILGANLVTPQTGSEGRMVRCLLVEEALD
                     IRRELYLSVLVDRVARAPLFMASMAGGMEIEEVANDRPESILREPIYPETGFQPYHAR
                     KIAFGLGLPADMVNAAVPFLQSLYRAFLETDASLLEINPCVITADGRLMALDAKVNFD
                     DNALYRHPELRELRDLDEETPLEV"
BASE COUNT          889 a         1141 c         1216 g          879 t
ORIGIN      
        1 gccgtgcggt tctccgcgtg ctctccactg cggatagccc gcatcgtttt agccgccgtg
       61 gcaggcgaac ttcgagatga tccaacccga tttcaaaact ttccgctgct tggcacagca
      121 gggaaatctc ataccggttt atgaaaccta taccgcggat ctcctcacgc ctgtaggcgc
      181 ttacctgcgg atcacacgcg attcaaaata ttcctttttg cttgagagcg tggaaggcgg
      241 cgaaacgatc gggcgttata cgttcgcggg tgtgaaccca gaagaagttt ttcgagctcg
      301 cggtaggcac tgcacgctcg aaaagggagg caaaaaagtt catttcgtcg aaaatccgat
      361 tgagcaattg cggaggctca cggctcagta ccgcacggtg cgcgtgccgg ggcttccccc
      421 actgattgcg ggggcaatcg gatatttttc ttacgatatg gtgcgactcg tagaacggat
      481 tccggacaca ggccgcgacg acgttgccgc agatgactgc gtaatgatgt tttatttagg
      541 cctcttggcg ttcgaccatg taaagcaccg agtatgggtg atccgaaacg tgtttatcga
      601 aggccctgga agcctgcgag ctaagtacga cgcggcgatt cgagagatac gccacacgcg
      661 gggcctgctc gaaaagcccc tggcaccgag gcgtcatgcg cgaaggggca gttccctgcg
      721 ggtccgttcg aatatgacaa agtcagcttt caccaacgcg gtggggaggg ccaaggaata
      781 cattcgagcg ggcgacgcgt ttcaaatcgt ggtgagccag cgcttcgagg cgcgaacaaa
      841 ggcaaatccg ctcgatatct atcgcgcgct gcgagtcgta aacccttcgc cgtacctcta
      901 ttatctacag ttgggtgaca tgtccgtggt gggaagctcg ccagagatgc ttgtaaaagt
      961 gcagggacgt gacgcctttt atcgccctct cgccggcacg cagccgcgcg gtcgcaatga
     1021 aatggaagat cgggagttgg aagaaaaact cctcgcagat ccaaaggaac gcgccgaaca
     1081 cgtgatgctg gtcgatctgg gccgtaacga cctcgggcgg gtttgcgaat acggctccgt
     1141 taaagcggag cggctcatgt tcgtcgagcg atactcccat gtgatgcact tggcatcgag
     1201 tcttcgagga cgcttgcggg gagatgtcga ttgcttcgac gcgctaatgg cttgttttcc
     1261 agcggggacg ctttcgggcg ctccgaagat ccgcgccatg cagatcatcg acgaacttga
     1321 gccgacgcgt cggggagtct atgggggtgc gatcctctat ctcgatttct ccggcaatct
     1381 cgattcctgt atcgggcttc gtacattgat tgcaaagaac gggcgagcgt atgtccaggc
     1441 aggggcagga atcgtggcgg attcggtgcc cgagcgcgaa tatgaggaat caggggacaa
     1501 agcgcgtgcc gtgctcaagg cgcttgaaat tgcacatcgt ggaggcagca aggaatagga
     1561 ctcgcttcac gcgacgaatt tgatttcgcg ctattctggc aaggccacca tgcttctact
     1621 tctcgataat tacgactcat ttacatacaa cctggcacag tatctcgggc agatgggcca
     1681 agcgctagaa gtccgccgta acgatcaaat tacgttggat gagattgagt atcttcagcc
     1741 ggaacggatc gtgatttcac cgggaccatg cacgccagcg gaagccggca tcagcgttcc
     1801 gttgatcgaa tgctttgccg ggaaaattcc gattctcggt gtttgcctgg gccaccaggc
     1861 gatcggggcg gcgttcggag gccgtgtgat cctggcggac tgcgtgatgc atggcaagac
     1921 gagtgagatc catcatgacg gcaagacgat ttttcgtgat ctaccgcaga attttcccgc
     1981 cacgcgctac cactctctaa ttgtggaacg gaaatcactg ccccccacgc tggaaatctc
     2041 ggccgaaacg gccgacggaa caatcatggg tctgcgtcac cggaaactca aagtggaagg
     2101 cgtgcaattc catcccgagt cggtgctcac ggaggccggt tttcagctgc tccagaattt
     2161 tctaagtatt taagaccccg ctgatgccat tcggctgagc ccgtcaggaa agagccaacc
     2221 ctatcgacac tatgggaaaa cagggccact ttcaatttgc gagaaggcta tgaaggcgtc
     2281 taggcgttcg cgcgccgcac ctgaatcgat cgactgggcc gcaagacgcg cgccttcgag
     2341 gaagttttcc gcgcggcccc ctgcgaccag agccgcagcc gcgttggcaa gaacaatttc
     2401 gcggtgcggc ccatggtcac gatagagcat cgaccgccct agaatcttgt gaacgatctg
     2461 tgcgttttgt ttcgagtcgc cgccgcgaat cgcttcggga ggggcgcctt gcaatccaaa
     2521 atcggcggga gtgactgtaa agctgcgtac ccgcccgtcc tgcagctcgg caacgtgggt
     2581 aacacccgag atagaaattt cgtcaaggcc gtcatcacca tgaaccacaa aggcccggcg
     2641 gacacccagt tcaccgagcg cccgagccat aagctcgatt acacctttgt cgtacacgcc
     2701 aacaagctgc gcggatgctc cggcaggatt cgttagcggg ccgagcaaat tgaagacggt
     2761 ccgcccattt agtctgcgcc gcgcaggcat tgcaaaccgg gtggccgcat gaacggcggg
     2821 agcgaaaaga aacccaatgc caacactctc tatacaacgg gcgaccgagc ccggtgtaag
     2881 atcaatgcgc ccgccaagct gctccaggac atccgccgaa ccgcagcgcg agctgatcga
     2941 gcggttgccg tgcttcgcca cgcgaactcc tgcccccgca actacgaatg ccgcaacagt
     3001 cgagacgttg aatgtcccac agccgtcgcc gccggttcca caggtatcga tgagagcctt
     3061 atccgcagct ggatggtttg gtcggaaaat cggtgtggcg tggcgccgca tcgcctttgc
     3121 aaagcccaca atttcatcca gtgtctcggt cttcgtctga aggcccttaa ggagaccgat
     3181 aatatgcgcg tttgtagcgc gcccggagag gatctcctcc atggctgcct cagcttcgga
     3241 acgcgacagg tccgttttcg cgtcgacttt tagaaccgcc tcatgcagca ttctttttgc
     3301 taccttgccc gtctcgcagc ttacaagtac actgaagtgc gttattggtt cataaattct
     3361 agcatttctc aagcctggtt ttgccggtgt cgagcccgcg atgaagatcc acgaatatca
     3421 agcgaaagca atcctcgcga aattcggcgt ttctgtgcct cgcggaaggg tggtgcgcaa
     3481 gaaggataag gcgcgagcgg cggcagagga attgggcttg ccggtggttg tcaaggctca
     3541 gattcacgct ggtgggcgcg gcaaaggcgg cggcgtgaag gtcgtcaagt cggccgatga
     3601 ggctgcgtcg ttcgctgagc acatcttggg cgcgaatctc gttactccgc agactggatc
     3661 ggaggggcgg atggtccggt gcttgctggt tgaagaagcg ctcgatatcc ggcgcgaatt
     3721 gtatctgagc gttttggtgg atcgcgtggc ccgcgcgcct ctctttatgg ccagcatggc
     3781 tggcggcatg gaaatcgagg aagttgcgaa cgatcgtccc gaatcaattc tgcgtgagcc
     3841 gatttatcca gagacaggtt tccagcccta tcacgcacgg aagattgcgt tcggcctggg
     3901 acttccggcc gacatggtta acgctgcggt gcctttcctc cagtcgcttt atcgcgcgtt
     3961 cctcgaaaca gacgcttctc tcttggagat caatccatgc gtcattaccg ccgacggccg
     4021 acttatggcg ctagacgcca aagttaattt cgacgacaac gccctctatc gtcatcctga
     4081 attgagggag ctgcgcgatc tggatgaaga aacgccgctg gaggt
//
LOCUS       DATNDT010000090         6786 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3249383, whole genome shotgun sequence.
ACCESSION   DATNDT010000090 DATNDT010000000
VERSION     DATNDT010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6786)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6786)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6786
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3249383"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..278
                     /locus_tag="VN788_02680"
     CDS             <1..278
                     /locus_tag="VN788_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19468.1"
                     /translation="GKLAKIFTVTRCEYISGLTCRIRYNGAAPLPSDVFFTEFDDMGR
                     QAVARVRLIYPELKTGETGVATLRIRSARPAKIVLRGEWNGPWRSPY"
     gene            complement(426..3332)
                     /locus_tag="VN788_02685"
     CDS             complement(426..3332)
                     /locus_tag="VN788_02685"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012222545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HXU19469.1"
                     /translation="MTTAANPTTAAQGKQQSENPLKALARFGQSVWLDYIRRNLLTTG
                     ELGRLIAEDGLRGMTSNPAIFEKAIAGSTDYAQALEELAKQKDLDAKAIFEHLAIRDI
                     QDAADILRPVYLQTKRRDGYVSLEVSPYLAHKTRETIDEARRLWSAVARENIMIKVPG
                     TPEGLPAIRELIGSGINVNVTLLFAQEVYEKVVEAFIAGLEDFARRDGDVSRVASVAS
                     FFVSRIDALVDSKLSELVKNPPPGIDRAFLNGLQGKVAIANAKLAYDLYKKIFDGPRW
                     QSLAQRGATTQRLLWASTSTKNPAYSDILYVEELIGPDTVDTIPPATFDAFRDHGKPR
                     ASLEENIDQARQTMSDLARAGISMKAVTDQLTEDGVKLFAEAFDKLLAAVEKSVQKTG
                     PSASGSLSYKLPPDLAQAVKSALDDWNANNKVKRLWARDATLWTNSDESKWLGWLSIT
                     EDQRSNADRFRQIAAEIASEGFTHAVLLGMGGSSLCVEVLKVTFGKISGHPQFLILDS
                     TSPTQIKAIESKLDLAKTIFIVSSKSGSTLEPNIFKQYFFERVKQVVGPAKAASHFIA
                     ITDPGSHMQKVAESDGFRGVFFGSPSIGGRYSALSNFGTIPGAIQGIDIPKFLDRTEE
                     MVHACASVVPADQNPGVILGAILGTLQKAGRDKVTIFTSPGISDLGAWLEQLLAESTG
                     KEGRGLIPVDREEIGAPDIYGNDRVFAYLRLSAAPDSRQDKSIDALEKAGHPVIRINV
                     PEIIDIGQEFFRWEIATAVAGSIIGINAFNQPDVEASKIETRKLTDQYEKSGSLPQES
                     PILEQDGIKLFTDEKNAAALKPPSGKDAMLAAYMRAHLDRLKPGDYFALLGFIQMNDA
                     HEAVLQSIRHAVRDKKRVATCLGFGPRFLHSTGQAYKGGPNSGVFLQVTCDYAPDLPV
                     PGQKYTFGIVQAAQARGDFQVLAERNRRALRVHLGPDVAAGLAKLREAITQALA"
     gene            3533..5017
                     /locus_tag="VN788_02690"
     CDS             3533..5017
                     /locus_tag="VN788_02690"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF004816.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HXU19470.1"
                     /translation="MDSAAAFSSTGASSDLDFASALQAAEAIRKKQISSVELTRRVFE
                     RIDRYDPALNAFVYQLRENALIRARAADEAVASGGVLGVFHGVPILVKESFAIAGHPC
                     TWGISALKDSKASRNAAAVDRLLGAGAIIVGATNVPVNLQDWQSYNPIYGTTNNPWDL
                     KRTPGGSSGGAAAALAAGLGYLAMGSDIGGSIRMPSNFCGIYGHKPTLDLVNLRGHMA
                     GGGFSALGFSTLLAVVGPMARSASDLLAALKVVGGPQDWDAKAWKWELPPPRGKDLKE
                     FRVGYVLDDPIASPVPEVAAVLEEAVEDLGRAGATMKPGWPTGFRPTALLENYLFMLS
                     AFGMSMAPPAEQETRKQKLANASDPMSVGALSSFADWQQQNLQRLAFRAQWQAYFDDV
                     DVFLSPVAFAIAFPHDHTEPQSDRSIATVGGPRKYMDFLNWIAPATLTGCPATVAPVG
                     RTQEGLPVGIQIMGPYWEDATTITFAELLAREVGGFVAPPGYDK"
     gene            complement(5060..6184)
                     /locus_tag="VN788_02695"
     CDS             complement(5060..6184)
                     /locus_tag="VN788_02695"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXU19471.1"
                     /translation="MPSATAAEIRSLAAGPLNWDLLLSNAAGHTVTPLLAKQLAAVAG
                     DALSAARLKQLKDLARAAVVRSLAFTAELVTILDLFRSEGLQAIPYKGPVLAEQAYGN
                     FALREFDDLDLILRQKDMPKANRLIASLGYRAKYPWTLSQDAAASLVPGEYCYRNERR
                     RMVVELHTERTLRHFPLPPDIGRLAERLVRVSLSGHAIETFAPEDGLPLLCIHGSKDF
                     WERISWVADVAEFVQANPRLDWDQVVRLAESLRAMRMLHLGLALAMRFFSLPLPGEIR
                     HRVEEDKIAGVLAEKVELRVFHDGANSFNAARRFWFRRRMIEGSFEGWRYSARLTVSP
                     AEEDWEMVRLPRPLAPLYVALRPLRLLVKYGASDTRPEHP"
     gene            6404..>6786
                     /locus_tag="VN788_02700"
     CDS             6404..>6786
                     /locus_tag="VN788_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXU19472.1"
                     /translation="MILRLPSEYLLSFAIIRHQSMDFDHLITFLEIAKQGSFSRAGQK
                     LYRSQPAVSAQIRQLEQEYGQKLFDRVGKTVRLTIAGETLLEYAARLLTLRNESLRAV
                     ADQSSTPHGTLALGANEATCLYVLP"
BASE COUNT         1393 a         1768 c         2182 g         1443 t
ORIGIN      
        1 gtggcaagct ggccaaaatt ttcacagtca cccgctgcga gtacatttcg gggctgactt
       61 gccggattcg ttacaatggc gctgccccac tgccgagcga cgtattcttc acggagttcg
      121 atgatatggg gcgccaggca gtggctaggg tgcggctaat ctatccggaa ctcaagaccg
      181 gcgaaaccgg tgtggcaacg ctccgaatcc gctcggccag gccagcgaag atcgtgctgc
      241 ggggtgaatg gaatggcccc tggagaagtc cgtactaatt ttgcagcctc tattcgttgt
      301 gagtgacggt acggaagaga tccaagagag attcttcgac tgcgtgtccc ggcgcttcgc
      361 gcaaaaccaa agacgcggga cactccgctc agaatgacgg cgttcacttt ttagttggcc
      421 ccaccttaag cgagggcttg ggtgatggcc tcgcggagct tggctaggcc ggcggctacg
      481 tcggggccga ggtggacgcg aagggcgcgg cggttgcgtt cggctagcac ttgaaaatcg
      541 ccgcgcgctt gagcggcctg cacgattcca aaagtatact tttgtcctgg caccggtaga
      601 tccggggcat agtcacacgt gacttggaga aacacgccgg agtttggacc gcccttgtag
      661 gcttgtcctg tggagtggag gaagcgcggg ccgaagccaa ggcaagtggc gacgcgtttt
      721 ttgtcgcgca cagcgtggcg gatagattgg agcacggcct cgtgcgcgtc attcatctgg
      781 atgaaaccga gcaaagcaaa ataatccccg ggtttgaggc ggtcgagatg cgcgcgcatg
      841 taggcagcga gcatggcatc tttaccagat ggcggcttca gggctgcggc gttcttttca
      901 tcggtgaaga gcttgatgcc atcctgctct aagatcggcg attcttgcgg aagcgagccg
      961 gatttttcgt attgatcggt tagcttgcgc gtttcaatct tgctggcttc gacgtccggc
     1021 tgattgaaag cgttgatgcc gataattgag cctgcgacgg ccgtcgcaat ttcccagcgg
     1081 aaaaattcct ggccgatgtc gatgatctcg gggacgttga tgcgaatgac gggatggccg
     1141 gctttttcga gcgcgtcaat ggatttgtcc tgcctggaat cgggagcggc gctcaggcgc
     1201 aggtaggcga agacgcgatc atttccgtag atatcgggcg cgccgatttc ctcgcggtcc
     1261 acggggatga ggccgcgtcc ttctttaccg gtagattcgg cgagcaattg ctctagccag
     1321 gcaccgagat cggaaatgcc gggcgaagtg aagatggtga ctttatcgcg gccggctttt
     1381 tggagcgttc caaggatcgc gccaagaatg acgccgggat tttggtccgc agggacgacg
     1441 gaggcgcagg cgtggaccat ttcctcagtg cggtcgagga acttgggaat gtcgatgccc
     1501 tgaatagcgc cgggaatagt tccgaaattc gaaagcgcgg agtaccggcc gccgatactc
     1561 gggctgccga agaacacgcc gcgaaaacca tcggactcag cgactttttg catgtgcgag
     1621 ccggggtcgg tgatggcgat gaaatgcgat gcggctttcg ccgggccgac gacctgcttc
     1681 acgcgttcga agaaatattg cttgaagata ttaggctcaa gggtgctacc ggatttgctg
     1741 gagacgatga aaatagtttt agctaaatcg agcttcgatt cgatggcttt gatttgagta
     1801 ggagaagtcg agtccaggat cagaaactgg ggatggccag aaatcttgcc gaatgtgacc
     1861 ttcaaaactt ctacgcacag gctggagccg cccatgccga gaagaactgc gtgggtaaag
     1921 ccctcggacg cgatttcggc agcgatttgg cggaagcgat cggcgttcga tctttggtcc
     1981 tcggtgatgg aaagccagcc gagccacttg ctttcgtcgg agttcgtcca cagcgtggca
     2041 tcgcgcgccc agagacgctt gactttgtta ttggcattcc aatcatcgag ggcggacttg
     2101 accgcctggg cgagatcggg ggggagctta taagacagac ttccggaagc tgacggcccg
     2161 gttttctgga cgctcttctc aacggcggcg agaagcttat cgaaagcttc ggcaaaaagt
     2221 ttgacgccgt cttcggtaag ctgatcggta accgccttca tggaaattcc ggcgcgggcg
     2281 agatcgctca tcgtctgacg agcctgatcg atattttcct cgaggctggc gcgcggttta
     2341 ccgtggtcgc ggaatgcgtc gaaggtcgcc ggaggaatgg tatcgaccgt gtcgggaccg
     2401 atcaattctt cgacatagag aatgtcgctg taggcgggat tcttcgtact ggtgctggcc
     2461 cagaggaggc gctgagtggt ggcgccacgt tgggcgagag attgccagcg ggggccatcg
     2521 aagattttct tgtatagatc gtaagcaagc ttggcattgg cgatggcaac tttgccctgc
     2581 aatccgttga gaaacgctcg gtcgatcccg ggcggcggat tcttgacgag ttcggagagc
     2641 ttgctatcga caagcgcatc gatgcggctg acgaagaaac ttgcaacgct ggcgacgcga
     2701 ctcacatcgc catcacgcct tgcaaaatcc tcaaggccgg cgataaacgc ctcaacaact
     2761 ttttcgtaga cttcttgcgc gaaaagaaga gtgacattca cattgatgcc gctgccgatc
     2821 agttcgcgga tggcgggcag gccctcgggc gtgccgggaa ctttgatcat gatgttctcg
     2881 cgagcgacgg cgctccagag gcggcgcgcc tcatcgattg tttcgcgcgt tttgtgcgca
     2941 aggtagggcg acacttccag gcttacgtag ccgtcgcggc gctttgtttg aagatagact
     3001 ggccgcaaga tgtcggcagc atcctgaata tcgcggatgg cgagatgctc gaagatggcc
     3061 ttggcatcga gatccttttg cttggcaagt tcctcgagcg cttgagcgta atcggtgctg
     3121 ccggcgatgg ctttttcgaa gatggcggga ttggaggtca tgccgcggag gccgtcctcg
     3181 gcgatcaggc ggccaagttc gccggtggta agcaggttgc gacggatata gtcaagccag
     3241 acggattggc cgaagcgggc gagggctttg agcggattct cggattgctg tttgccttgc
     3301 gcggcggtgg ttggattcgc ggcggtggtc atagtgtccc ccagagaaac aaacaacgat
     3361 tttagcgcag gtgtaggctg ggcgcgaatt tcctgcgttg tggacaatgt ggccgcaggg
     3421 gcgcagtcgc ggcgaagttt tttgtggcct tgcctggacg cttgtcttcg aggcaaattt
     3481 tgttgtgcac ggggaatgag aagcgtacga tgcgtcccct aagaggtgct acatggattc
     3541 agcggcggcg ttttcgtcca ccggagcttc cagcgatttg gattttgctt cggcattgca
     3601 agcggctgag gctattcgca agaaacagat ttcgtccgtc gaattgactc gccgtgtttt
     3661 cgagcggatc gaccgctacg atccggcgct caatgcgttc gtttaccagt tgagagaaaa
     3721 tgcgctgatt cgcgcgagag cggctgacga agcggtggcg agcggaggag ttctcggtgt
     3781 gtttcatggc gttcccattc tggtgaaaga gagcttcgct atagcgggcc atccatgtac
     3841 gtggggaatc tccgctttga aggactcgaa ggcgtcgcgg aacgccgctg ctgtggaccg
     3901 gctgttgggt gcgggagcga ttatagttgg cgcaaccaac gttccggtaa accttcagga
     3961 ttggcagtcc tacaatccga tttatggaac gaccaacaat ccctgggatt tgaagagaac
     4021 gccgggcggg tcttcaggag gagcggcggc ggcgctggcg gcggggctcg ggtatcttgc
     4081 aatgggaagc gatatcggcg gctcgatacg catgccgtca aatttttgcg ggatttatgg
     4141 gcacaagccg accctggacc tcgtgaatct tcggggccat atggcgggcg gcggtttctc
     4201 cgcgctgggt ttctcgacgt tgctcgccgt cgttggacct atggcgcgaa gtgcctccga
     4261 tctgctggcg gcgctgaaag tcgtgggagg gccgcaggat tgggatgcca aggcatggaa
     4321 atgggaattg ccgcctccac gcggcaaaga tttgaaggag tttcgcgtcg gatatgttct
     4381 ggatgatccg atagcttcgc cggtgccaga agttgcggcg gtgttggaag aggcagttga
     4441 ggacttgggc cgggcaggag caacgatgaa gcccgggtgg cccacgggat tccggccgac
     4501 ggcgctacta gaaaattatc ttttcatgct gtccgcgttc ggcatgagca tggctcctcc
     4561 ggctgaacag gaaacgcgaa aacagaaact cgcaaacgct tcagatccca tgtccgtcgg
     4621 agccctgtcc tcattcgcgg attggcagca gcagaacttg cagcgattgg cgttccgggc
     4681 gcagtggcag gcttacttcg atgatgtgga cgttttcctc tcgcctgtcg cgtttgctat
     4741 agcgttccct cacgatcaca ctgagcccca aagcgaccgt tcgattgcca cagttggcgg
     4801 gcctcgaaaa tacatggact tcctgaactg gattgcgccc gcgacgctta ccggctgccc
     4861 ggccaccgtc gcgcccgtcg gaagaaccca agaaggactg cctgtcggaa ttcagatcat
     4921 ggggccgtat tgggaagacg ccacgacgat cacctttgca gaattgcttg ctcgcgaggt
     4981 gggcgggttt gtagcgccgc cgggatatga caagtagctc tcccaaggcc gtcgcaatgc
     5041 ttcctgtgcg atgacgaagc taggggtgct cggggcgtgt gtccgaggct ccatacttta
     5101 caagcaagcg tagcgggcgg agcgcgacgt agagcggagc gagaggacga gggaggcgga
     5161 ccatttccca gtcctcttcg gcaggcgaaa ccgtcaggcg ggcggaatag cgccagccct
     5221 caaaacttcc ttcgatcatt cgacgccgaa accaaaatct cctggcagcg ttaaatgaat
     5281 tcgcgccgtc gtgaaaaact cttagctcga ctttctccgc aaggacgccg gcgattttat
     5341 cctcttccac gcggtgccga atttcgcccg gcagcggcag gctaaaaaaa cgcatcgcga
     5401 gcgcaagccc gagatgaagc atgcgcatcg cccgcagcga ctccgcgagg cggacgactt
     5461 ggtcccaatc aagccgcgga ttggcttgga caaactccgc gacgtctgca acccaactga
     5521 tgcgctccca gaagtccttc gatccatgaa tgcagagaag gggaaggcca tcttcgggag
     5581 caaaggtttc gattgcgtgt ccgctgagag agacgcggac gagacgctcg gcgagcctcc
     5641 cgatgtccgg cggcagcgga aagtggcgca acgtgcgctc cgtgtgaagc tcaacgacca
     5701 tgcggcggcg ctcattgcga tagcaatatt cgcctgggac gagcgacgcg gccgcgtcct
     5761 gtgatagagt ccagggatat ttcgcacgat agccgaggct ggcgatcaga cgattcgcct
     5821 tgggcatgtc cttctggcgc agaataaggt cgaggtcgtc aaactcgcgc agggcgaaat
     5881 ttccgtaggc ttgctcggcg agaacggggc ctttgtaggg aatcgcctga aggccttcgg
     5941 agcggaagag atctaggatc gtgacaagct ccgcggtgaa ggcaaggctg cgaacaacgg
     6001 ctgcgcgcgc gagatctttc aactgcttga ggcgtgcggc agagagtgcg tcacctgcca
     6061 cggcagcgag ttgcttggcg aggagaggcg tgaccgtatg acctgcggca ttgctcagaa
     6121 gcaaatccca attgagcggg cctgcggcca gcgagcgaat ctcagccgcg gttgctgagg
     6181 gcatcttcgt ccgcgtgcag cagacaagca gccgtttttc tgaagataca ctagcgctca
     6241 aggagtgacc gtcccttacc gcgcaaaacg agagccgcca caattttacg atatcgccag
     6301 ggcgaccgaa gaaattcttc ggaatgttag cttgcgagca ccggattgtc gcgggcgcaa
     6361 tgggtgctca taacggaata gcgagcgaag tcgtgacggc cgtttgattt taaggcttcc
     6421 gagtgagtat cttcttagtt ttgctataat tcgtcaccaa tccatggact tcgatcacct
     6481 aatcactttc ctggaaattg ccaagcaggg cagcttttct cgcgcgggcc agaagctcta
     6541 ccgttcgcaa cctgccgtga gcgcgcagat caggcaactg gagcaggagt acgggcaaaa
     6601 gcttttcgat cgagtgggaa agacggtgcg cctgaccatt gccggagaaa cgctgctcga
     6661 atatgcggca aggttgctga cactgcgcaa tgaatcccta cgcgcagtgg cagatcaatc
     6721 gagcacgccg cacggcacgc tggcccttgg cgcgaacgaa gcgacctgcc tctacgtgct
     6781 gccgga
//
LOCUS       DATNDT010000091         5803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_528048, whole genome shotgun sequence.
ACCESSION   DATNDT010000091 DATNDT010000000
VERSION     DATNDT010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5803
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528048"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1306
                     /locus_tag="VN788_02705"
     CDS             <1..1306
                     /locus_tag="VN788_02705"
                     /inference="COORDINATES: protein motif:HMM:NF014879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HXU19473.1"
                     /translation="GHLRAHTKAPVVIVGYDTRFFSEDFARTSAAVLRSDGCRILLCG
                     APTPTPAIAHAIICGKLDGGVNITASHNPAQYNGLKFSGSDGGPALPEVTRDLEKRAA
                     KLEDEPGQDHEIEDTFERIDPREAYFEQLCRVIRFDVLKKAKGNFVCDAVHGCGAGWL
                     DRILSENGLGVTSIRANRDVLFDGTGPDPSEENLAPLKSAVREKRALAGLATDGDADR
                     FGIIDGDGVFISPNHILALCFDYLLETRGYKLGASRSVATTHLIDAVAKLHGVRVYET
                     PVGFKYVGPLLREDKIALGGEESAGMTIRGHLPEKDGILACLLVGEMIAARQTSLSDQ
                     LHDLFRRVGREFWPVRENLHVSDDVQAKLPNRLKNDFKEFAAHRVSRTDRTDGLKLIF
                     DDDTWLLMRLSGTEPIVRVYAEAATPAASKKLADEAREWVSP"
     gene            1303..1677
                     /locus_tag="VN788_02710"
     CDS             1303..1677
                     /locus_tag="VN788_02710"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HXU19474.1"
                     /translation="MSEQSISFYEQMRNFLRQNLNWFLALGLALLLLQDVFGTHGLLA
                     MRRAQKESARVQREIDQINQENRRLQENVKALKTDPAAIERIAREEMGLARPGEYIFK
                     LPAKPGDAPALVSPPSDSAAKP"
     gene            complement(1674..2801)
                     /locus_tag="VN788_02715"
     CDS             complement(1674..2801)
                     /locus_tag="VN788_02715"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HXU19475.1"
                     /translation="MVQSGTGRLAYLDWLRGFACLGMFEVHCYDAWLGGAARHSGFFG
                     LTQFSGTLPAPLFIFLAGISSALVTDRMRRKGAKPGAIAKRIMRRGAEIFALGLVFRV
                     QEYCLGQPWAPSTDLLRVDVLNMIGISIVLVGIPCGLVQSRKINIALAASASIGMALI
                     TPPLWTTWRAHWMPWYLESYINGVHTYTNPQPWLFPIFPWTAFAFAGLATGFLLTCDW
                     ARRNASQMATILGGSGIAIFFVSRWLDAAPMQLYSVYDYWHTSPNFFFARVGIVLVVV
                     WVAYAWCQWGLGAKGFSPLIQLGQTSLLVYWVHIEFVYGRFSILAQHSQGIAMATLGL
                     LIIFAAMLMLSMARTRTKGRGAEILARLRWRRGMPSVARET"
     gene            2891..3871
                     /locus_tag="VN788_02720"
     CDS             2891..3871
                     /locus_tag="VN788_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXU19476.1"
                     /translation="MTPDSRSRQITFAAVAAIWAVIVSMAAFAGLWMGFGGRRFAVAL
                     IVAAVLFGFELFLAVPSVLKLALFSLRIPGILLAPLVPLFAVLIYSFTVSGNLKTVLA
                     GAAYAVLPALLLARSTGKSPGRWEDYAAAIVVWMPVEFRWMYKIFPYPPPLTHVLTIL
                     MALGTGVAAFVLLRRLEGIGYAIDWRRGFGWIVALYFIIFAVIAIPIGIKTHFLAFAP
                     SLHRQHFFPLTALGIVFFTAWPEEFLFRGILQNLISSTMKNEWAGLAMTSIIFGLSHI
                     FHAPYPNWKYMLLATIAGLFYGHAWMRTRSLVPGTLIHAFVDISWHILFR"
     gene            complement(3912..4688)
                     /locus_tag="VN788_02725"
     CDS             complement(3912..4688)
                     /locus_tag="VN788_02725"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HXU19477.1"
                     /translation="MTDQLVIAGRAFRSRLIVGTGKYRSFQEMARAHAASGAAMVTVA
                     VRRVNLTDRSKESLLDYIDREKLFILPNTAGCYSADEAVRAARLGREVGLSEWVKLEV
                     IGDEQTLFPETEELVRATKTLVKEGFIVLPYTNDDLIVARKLIDAGASAVMPLGAPIG
                     SGMGIQNLANLRILRERITEVPLIVDAGVGTASDAAIAMELGADGVLMNTAIAGAQDA
                     VLMAEAMRAAVEAGRKAFLAGRIEKKLYATASSPAAGVVR"
     gene            complement(4748..>5803)
                     /locus_tag="VN788_02730"
     CDS             complement(4748..>5803)
                     /locus_tag="VN788_02730"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXU19478.1"
                     /translation="QALLEVRPTTMAAVPRFYEKTYANIIEKGHRESGLKRKIFDDAM
                     RVAAKAVPWRAHGKSVPWALKLRWWVADKLVYSKIHQGVGGRIRIFSSGGAPLAPELA
                     EFFWSIDLPVYQGYGLTETSPVVTFNSPKMNKVGTVGRPIPHVQLRLANDGEILVKGP
                     CVMQGYYRKPEETRAVLTPDGWFSTGDIGRIDADGFLTITDRKKELLKTAGGKFVAPA
                     PIENLLKTSPLISNAMVVGDRRKFVSVLVVVNFAGVEEAARKAGKDFPTAAQAIGDPW
                     VRELMSHEIERLTSSLAQFEKPKRFALLEQDFTYANGELTYTLKLKRRAIEQRYQDTI
                     ARLYADVEEPHPQPAAG"
BASE COUNT         1224 a         1792 c         1539 g         1248 t
ORIGIN      
        1 tggccatctt cgcgctcaca ccaaagctcc tgtggtgatc gtgggctacg acacccggtt
       61 tttttccgaa gacttcgcgc gaacgtccgc cgccgttctc cgctcggatg gttgccggat
      121 actgctgtgc ggtgcgccta cacccactcc cgctattgcc catgccatca tttgcggaaa
      181 actggacggc ggcgtaaaca tcaccgcgag tcataatcca gcgcaataca atggcctgaa
      241 attttccggg tccgatgggg gaccagccct accggaagtc accagggacc ttgaaaagcg
      301 ggcggctaaa ctggaggacg aaccgggcca ggatcatgaa attgaagata cttttgagcg
      361 tatcgatccc cgtgaggcgt acttcgaaca gctttgccgc gtgattcgtt tcgacgttct
      421 gaaaaaagcg aaaggcaatt ttgtgtgtga cgccgttcac gggtgcggcg cgggctggct
      481 cgatcggata ctctctgaaa atggcctggg cgtcacttcc attcgcgcga accgcgacgt
      541 gctcttcgac gggactggcc ccgacccgtc cgaagaaaat ttggcgccat tgaagagcgc
      601 cgtgagagag aaaagagctc ttgcaggact ggcaaccgac ggtgatgccg accgtttcgg
      661 cattatagac ggtgacggcg tgttcatttc gcccaatcat atcctggcgc tttgctttga
      721 ctatctgctc gaaacgcgcg gatacaagct gggcgcctcg cggagcgtgg ccaccacgca
      781 cctgatcgac gcggtcgcga aattgcatgg cgtgcgagtg tatgaaactc cggtaggttt
      841 caaatacgtg ggtccattgc tgcgagaaga taaaatcgcg ctgggcggag aagagagcgc
      901 cggcatgacc attcgtgggc atcttcccga gaaggatggc attctggcat gtttgctcgt
      961 cggtgaaatg atcgctgcgc gccagacatc actttcggat caattgcacg atctctttcg
     1021 ccgagtgggc cgggagttct ggccggttcg ggagaacctg cacgtatcag atgatgttca
     1081 agccaagttg ccaaatcgtc taaagaacga ctttaaggaa ttcgccgcgc atcgcgtctc
     1141 gcgcacggat cgcacggatg gtctgaaatt gattttcgat gacgacacct ggcttctaat
     1201 gcggctctcc ggcaccgagc ctattgttag ggtttacgcg gaggccgcca cgcccgccgc
     1261 ttccaaaaaa ctcgccgacg aagctcgcga gtgggtttcc ccatgagtga gcaatcaata
     1321 agtttttacg agcagatgag aaattttctg cggcaaaatc tcaactggtt tcttgccttg
     1381 gggctcgcat tactcctgct acaggacgtg ttcgggacgc acggcttgct cgcgatgcgg
     1441 cgagcccaaa aggaatcggc cagggttcag cgcgaaatcg accagattaa tcaggaaaat
     1501 cgtcggctgc aggagaatgt caaggcgctg aagaccgatc cggcggccat cgagcgaatc
     1561 gcacgcgagg agatgggcct cgcccgtccc ggcgaataca ttttcaaact tccagccaag
     1621 ccaggtgacg caccagcgct tgtttcccct ccgtctgatt ccgccgcaaa gccctaggtt
     1681 tctcgcgcca cgctcggcat tccgcggcgc cagcgcaaac gcgcgagaat ttccgcacct
     1741 cgacctttcg ttcgcgttcg cgccatcgac agcatcagca tcgccgcaaa gattatcaga
     1801 agaccgagcg tcgccattgc gatcccttgg gagtgctgtg cgaggatcga aaagcgacca
     1861 tagacgaact cgatgtgcac ccaataaacc aacagcgagg tttgtccaag ttgtatcagc
     1921 gggctaaaac ccttggcgcc caatccccac tggcaccacg cgtacgccac ccagacaacc
     1981 accaatacaa tccctacgcg tgcgaagaag aaattcgggc tcgtgtgcca gtaatcgtaa
     2041 acgctataca gctgcattgg cgccgcatcc agccatcgcg aaacaaaaaa aattgcgatc
     2101 cccgagccgc ccagtattgt cgccatttgc gatgcattgc gccttgccca gtcgcaggtg
     2161 agcaagaatc cagttgccaa cccggcaaac gcgaacgccg tccagggaaa tatcggaaaa
     2221 agccatggct gggggttcgt gtaagtgtgc acgccgttga tgtacgactc gagataccac
     2281 ggcatccaat gcgctcgcca agtggtccag agcggcggcg taatcaacgc cataccaatt
     2341 gaggccgacg ccgcgagggc gatgtttatc tttcggctct gcaccaagcc gcacggaatt
     2401 ccgacgagca cgatcgaaat tccgatcata ttcaacacgt ccacccgcaa taggtcggtc
     2461 gaaggcgccc acggttgacc caagcagtac tcctgcactc gaaaaacgag accgagcgcg
     2521 aaaatttccg ctccgcgacg catgattcgc tttgctattg cgccaggctt cgcgcccttg
     2581 cggcgcatgc ggtccgttac caatgcggaa gaaatgccgg caagaaatat gaatagggga
     2641 gctggaaggg tgccgctaaa ttgggtcaat ccgaagaagc cgctgtgccg ggccgccccg
     2701 ccgagccatg catcgtagca atgcacttcg aacatgccga gacaggcgaa cccccgcaac
     2761 caatcaaggt aagcaaggcg tcccgttcca gattgcacca tggcgcgcgg cacgcctcag
     2821 aatcgccgag accgaaaatc aaatcaagct cttatcgggc agtcggactc tccggttaga
     2881 atcacttacc atgaccccgg attctcggtc acggcaaata acttttgcag cggtcgcagc
     2941 catctgggcc gtgatcgttt caatggcggc ctttgcgggc ctgtggatgg gatttggcgg
     3001 gaggcgattc gcggtggcgt tgattgtcgc cgccgtcctt tttggattcg aactgttcct
     3061 cgcagttccg agcgtactca agcttgctct attctcgctc agaatcccgg gaatactgct
     3121 tgcgccgctt gtgcccctgt tcgcagttct tatttattcg tttacagtca gcggcaattt
     3181 gaagacggtt ctggcaggag ctgcctatgc cgtacttccc gccctcctgc ttgccagaag
     3241 caccgggaaa tcgccaggca gatgggaaga ctacgccgcg gccattgtgg tttggatgcc
     3301 tgtcgaattc cggtggatgt acaaaatatt tccctatcca ccgccgctaa cgcacgtcct
     3361 cacgatcctg atggcgctcg gcaccggcgt tgcggccttt gtgctgcttc gcagactgga
     3421 aggcataggc tacgccatcg actggcgtcg cggcttcggc tggattgtgg cgctgtattt
     3481 catcatcttt gcggtcattg ccatccccat cggcataaaa acgcatttcc tcgccttcgc
     3541 gccgtctttg caccgtcagc atttctttcc attaacggca ttgggaattg tgttctttac
     3601 agcttggcct gaggaattcc tatttcgcgg cattttgcaa aatctgattt cgagcaccat
     3661 gaagaacgaa tgggccggac tggcaatgac atcaataatc ttcggactct cgcatatctt
     3721 ccacgcaccc tatcccaact ggaagtacat gttgctggcc acgatcgccg gactatttta
     3781 cggccacgcc tggatgcgaa cccgctcgct cgtccctggc accttgattc acgctttcgt
     3841 ggatatttcg tggcatatcc tatttcgctg aaggcgcgcg atggtgttga cgtcgcgcct
     3901 cacgcggaac cttacctcac gacgccggcc gcggggctgg acgccgtcgc atacaacttt
     3961 ttctcgatgc gcccggctag gaatgccttc cggcccgcct cgaccgctgc ccgcatcgct
     4021 tctgccatca gcaccgcgtc ctgcgcccct gcaatcgctg tgttcatcag cacaccgtcc
     4081 gcgcctagct ccattgcaat cgctgcatcc gatgccgtac cgacgccggc atcgacgatt
     4141 aacgggacct ccgtgattcg ctcgcgcaaa atgcgcagat tcgccaaatt ctgaattccc
     4201 atgccggacc cgatcggcgc gcccaaaggc atcacggccg atgcgcccgc atcaatcagc
     4261 ttacgcgcca caatcaaatc gtcgttggtg tacggcagaa cgatgaatcc ttccttaact
     4321 aacgtcttcg tcgcccgcac caactcttca gtctcgggga aaagagtttg ctcgtcgccg
     4381 atcacttcga gcttcaccca ttcagaaagg cccacctcgc gtcccagtcg cgctgcgcgc
     4441 actgcttcat cggccgaata gcatcccgcc gtgttaggca ggatgaacaa tttctcccga
     4501 tcaatgtaat cgagcaagga ttccttgctg cggtcggtca agttcacgcg ccgcaccgct
     4561 accgtcacca tcgccgcacc cgatgccgca tgcgctcgcg ccatctcctg aaagctgcgg
     4621 tatttcccgg tccccacaat cagccgcgat ctaaatgccc gaccggcaat caccagttgg
     4681 tcagtcatgt ctcactccgc tgatattcta ctccgcccgc catattttcg cggtatggag
     4741 gggaaggcta cccggccgcc ggttgaggat gcggttcttc cacatccgca taaagccgcg
     4801 caatagtgtc ctggtaacgt tgttcgatcg cgcgccgttt cagcttcagc gtgtaggtca
     4861 actccccgtt cgcgtacgtg aaatcctgtt ccagcagcgc gaagcgcttc ggcttctcaa
     4921 attgcgccag cgatgaagta agccgctcga tttcgtgcga catcaactcg cgtacccacg
     4981 gatcaccaat cgcttgcgct gccgttggaa agtccttgcc cgctttgcgc gctgcctctt
     5041 cgacacccgc gaagttcaca accaccagca ccgagacgaa tttccgccgg tcgccgacca
     5101 ccatggcatt tgagatcagc ggcgaagtct tcagaagatt ttcgatcggc gccggcgcca
     5161 caaatttccc gcctgcggtc ttgagcaact ccttctttcg atcagtgatg gtcagaaaac
     5221 cgtctgcatc gatccggccg atgtctccgg tggaaaacca tccatctggg gtgagcaccg
     5281 cgcgcgtctc ttccggcttg cgatagtagc cctgcatcac gcacggcccc ttcaccagga
     5341 tttctccatc gttggcaaga cgcaactgca catggggaat cggacgcccg accgtgccca
     5401 ccttattcat cttcggcgaa ttaaatgtaa ccactggcga ggtttccgtc agcccatagc
     5461 cctgatacac gggcagatca attgaccaga agaattctgc cagctccggc gccagcggcg
     5521 cgccacccga actgaaaatt cggatccggc cacccacacc ctggtgaatt ttcgaataca
     5581 cgagcttgtc tgcaacccac caccgcaact tgagggccca aggaacgctt ttgccgtgcg
     5641 ccctccacgg cacggccttc gcggccacgc gcatcgcgtc gtcgaagatc ttgcgtttca
     5701 accccgattc tcgatgccct ttctcgatga tattcgcgta ggtcttttcg taaaagcgcg
     5761 gcacggccgc catggtcgtc ggccggacct ccagcaacgc ctg
//
LOCUS       DATNDT010000092         3987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1826352, whole genome shotgun sequence.
ACCESSION   DATNDT010000092 DATNDT010000000
VERSION     DATNDT010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1826352"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VN788_02735"
     CDS             <1..546
                     /locus_tag="VN788_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19479.1"
                     /translation="ARGISPAIAELAGEPARAPLYKVVFDERFPASRKFASEAKSLGV
                     AVQGMKGDITDFWFNDLYFRWKQGPAAIAGLTAHGPIFCLERLAWDQRMRVVFRADHR
                     YREDGSIEHALSGPESMLHEATKLNSDGANWITRMASLVTRCPASRSQSSQANVIAST
                     VKHADDPDHLISWVIAPISRA"
     gene            583..2211
                     /locus_tag="VN788_02740"
     CDS             583..2211
                     /locus_tag="VN788_02740"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXU19480.1"
                     /translation="MKVPPGVSPQDFSDALKQFEDIVGKDWVFTSDQDVAMYRDAYSP
                     FYGEEDELVASAALAPDNVEDVQKIVKVANTYKIPIYTISTGKNLGYGGSAPAYSGSV
                     VLDLKRMNRILEVNEKSAYALVEPGVSYFDLYRYIQEKGLKLWVDVPDPGWGSPVGNS
                     LDRGGGYLYKEYRNHFDSHCGMEVVLANGELLRTGMGAMPGAKTWQQYKSGFGPWIDG
                     IFSQSNFGVVTKMGFWLLPAPDSYLAGTVTVPRYNDLTPLIEIMDYLENTGATQGMPF
                     IGSPLLARGPLGQGGPVPDPELTSMLADPNGPSPEAMDAYATKKGIHHWSCTLGFYGA
                     PKANEANWEFSKEKFSAIPGAQFKDSGTMKLPLTPEQQAHFNLPEFGIPSLQMFSIGA
                     RSATNPTPGEGHIWFSPIIPRNGEAVFEANRVFRQAAKDLDLPVPAFSLPTCYWNRAF
                     IFLFGFPITHDIETNKKNRAKFKKLVKIAAEHGWGEYRTAPAYQAAVMDTYSFNNHAL
                     LRFHETLKDAVDPNGILSAGRYGIWPKHLREAKS"
     gene            2208..2600
                     /locus_tag="VN788_02745"
     CDS             2208..2600
                     /locus_tag="VN788_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011236013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXU19481.1"
                     /translation="MKNFYACLIVASAFIFVAQAAAAQQQPARSSAWTRATVNGQGEP
                     KGYVQFQNYCSMCHGEGPGQPGTMALQAKYKGAQPALLSQRTDLTPQLIKLYVRNGIS
                     IMPIFRKTEISDADLDLIADYLTRNNKK"
     gene            2639..>3987
                     /locus_tag="VN788_02750"
     CDS             2639..>3987
                     /locus_tag="VN788_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXU19482.1"
                     /translation="MRLPPGVSSKDFSSAIDQFVNAVGKEWVFTSDDDVNTYRDSYSP
                     FWHEAEEPIPSAAVAPDGVEQVQAVVKIANNFKIPLWIISTGKNLGYGGSAPLLGGSV
                     VLDLKRMDRIIEVNEKNHYALVEPGVSYFDLYRYIQDKGLKVLIDPADPGWGSLIGNG
                     VDRGGGRTPMRDHFDAHCGMEIVLPDGEVVRTGMGALPDSKTWQQYRYGFGPYVDGLF
                     SQSSLGVVTKMGFRLFPQPEAYLTGTVTVMKRDDLIPLVAGLAYLMNSGIVLGTTSVA
                     NPLAFARQDPALAALYAKPGGASTEELEQYAQEKNLGMWSIELPFYGPAKVVAAQWEH
                     AKEKLSAIPSAKFTEGTTHRFPIKPDELPQFNSVAVGIPSLSVFGVGSARSKGHIWFS
                     PIIPMTGEAILEAQKVFTDAYKELGMSMGPMFFLGPSSNYPRCFLVLFGFPIEHDVE"
BASE COUNT          893 a         1154 c         1103 g          837 t
ORIGIN      
        1 gcgcgcggca tttcgcccgc gattgctgaa ctggccggag aaccggcgcg cgcgcctctt
       61 tacaaagttg tatttgacga aagattcccc gccagccgca aattcgccag tgaagcaaag
      121 agcctcggcg ttgctgtcca aggaatgaag ggcgacatca ccgatttctg gttcaacgat
      181 ctgtactttc gttggaagca aggcccggcg gccattgcgg gactcaccgc gcacggccct
      241 atcttctgcc tcgaaagatt ggcgtgggac caacgaatgc gtgtcgtttt tcgcgccgac
      301 catcgctacc gcgaggatgg ctcgatcgag cacgctctct ccggccctga aagcatgttg
      361 catgaggcga caaagctgaa tagcgacggc gcgaattgga tcacccgtat ggcgagcttg
      421 gtaacgcgct gccccgccag tcggtcgcag agttcgcaag caaatgtgat cgcatccaca
      481 gtgaaacacg cggatgaccc ggaccacctc atttcctggg tcattgcgcc gatcagtcga
      541 gcgtaaaacc gagcatagag cggcccgctc tcaggagttc cgatgaaagt tccgcctggc
      601 gtgagtccac aggacttttc cgacgcactc aagcagtttg aagatattgt gggcaaagac
      661 tgggttttta ccagcgatca ggatgtggcc atgtatcggg acgcatattc gcccttctac
      721 ggtgaagaag atgaactggt ggcgtcggct gctctcgcgc cggataacgt cgaggacgtg
      781 caaaagatcg tgaaagtagc gaacacttac aaaattccga tctacaccat ctccaccgga
      841 aaaaacctcg gatacggcgg ctccgctccg gcgtattccg gcagcgtcgt cctcgatctg
      901 aagcgcatga accgcatcct ggaagtaaat gagaagagtg cctatgcgct ggtcgagccg
      961 ggcgtcagct acttcgatct ctatcgctac attcaggaaa agggactgaa gctttgggtc
     1021 gatgttcccg atcccggctg gggcagcccc gtgggcaatt cgctggatcg cggcggcggt
     1081 tatttataca aggaatatcg caatcatttc gattcgcact gcggaatgga agtggtccta
     1141 gccaatggcg agttgctgcg caccggcatg ggcgccatgc ccggagcgaa aacctggcag
     1201 cagtacaagt caggattcgg tccgtggatt gacggcatat tttcgcagtc taatttcgga
     1261 gtcgtaacga aaatgggatt ctggcttctt cccgcgccgg attcctacct tgcgggcacc
     1321 gtcactgtgc cgcgctacaa cgatctgact ccgctcattg aaattatgga ttatctcgag
     1381 aacaccggcg ccacccaggg tatgcctttc atcggcagcc cactgctcgc ccgcggacct
     1441 ctgggtcaag gtggcccggt gcctgacccg gaactaacct ccatgcttgc cgatcccaac
     1501 ggcccctccc ccgaagcgat ggacgcgtac gccacgaaga aaggcattca ccactggagt
     1561 tgcacgctgg gcttttacgg cgctcccaaa gccaatgaag ccaactggga attttccaaa
     1621 gagaaattct cggccattcc aggagcccaa ttcaaagaca gcggaaccat gaagctgccc
     1681 ttgactcccg agcagcaagc gcatttcaat ctgccggagt tcggcattcc cagcttgcaa
     1741 atgttttcca tcggcgcgcg ctcagccacg aatcccacgc ccggcgaagg acacatctgg
     1801 ttctctccaa tcattcccag aaacggagaa gctgttttcg aggccaatcg cgtgtttcgc
     1861 caggccgcga aggacctaga cctgcccgtc ccagccttta gcctcccgac ttgttattgg
     1921 aatcgcgcct ttatcttcct ttttggtttc ccgatcacgc acgatatcga aaccaacaaa
     1981 aagaatcgcg ccaagtttaa aaagctggtg aagatcgccg ccgagcacgg ttggggcgaa
     2041 tatcgcaccg cgcccgcgta tcaagcggcg gtgatggaca cctactcctt caacaaccac
     2101 gcgctgctgc gcttccacga aactctcaag gacgccgttg atccgaacgg cattttatcc
     2161 gccggccgct acggcatttg gccgaagcat cttcgggagg ccaagtcatg aagaatttct
     2221 atgcatgcct gatcgtggcg tcggccttta ttttcgtagc gcaagccgct gccgcgcagc
     2281 aacagccggc aagatcctcg gcttggactc gtgcaaccgt gaatggtcag ggcgagccca
     2341 agggctacgt gcaattccag aattattgtt cgatgtgcca cggcgaaggt cccggacaac
     2401 ccggcaccat ggccctgcaa gcgaagtaca aaggcgcgca acccgcgctc ctttcgcagc
     2461 gcaccgattt gacgccgcaa ttgatcaagc tctacgtccg caacggcatc tcgatcatgc
     2521 caatttttcg caagacggag atcagcgacg ccgatttgga cctcatcgcc gattacctga
     2581 cgcggaacaa caagaaatag cattacgcat ctgattcgaa gggaaacgag agctgggaat
     2641 gagattgccg ccgggcgtca gcagcaaaga tttttccagc gcgatcgatc aatttgtcaa
     2701 tgcggtcggc aaagaatggg tattcacctc cgacgatgac gtaaatacct atcgcgattc
     2761 ctattctcct ttctggcacg aagccgagga acccattcct tccgcagccg tcgcgccgga
     2821 cggcgtcgag caggtccaag ccgtcgtcaa aatagccaac aatttcaaaa ttcctctgtg
     2881 gataatttcc actgggaaaa atctcggata tggcggctct gctccgctac tgggcggaag
     2941 cgtggtcctc gacctgaagc gcatggaccg gatcatcgag gtcaacgaaa aaaatcacta
     3001 cgccttggtc gagccgggcg tcagctattt cgatctctac cgctacattc aggacaaagg
     3061 actgaaagtt ctgatcgatc ccgccgatcc tggctggggg agtttgatag gcaacggcgt
     3121 ggatcgcggc ggcggacgca cgccgatgcg cgaccacttc gacgcgcact gcggtatgga
     3181 aattgttctg ccggatggag aagtagtgcg cacgggcatg ggtgcgctgc ccgactctaa
     3241 aacgtggcag caataccgct acggttttgg cccgtacgtt gatggactct tctcgcaatc
     3301 gagcttgggc gtggtcacca aaatggggtt tcggttgttt ccgcaacccg aggcgtacct
     3361 caccggcact gtaacggtga tgaaacgcga cgatttgatt ccgctggttg cggggctagc
     3421 gtacttgatg aattccggca tcgtcctcgg caccacgagc gtcgctaacc cgttggcgtt
     3481 cgcgcggcag gatcccgcac ttgcggcact ttacgccaag cccggcggcg cttccacaga
     3541 agaactagag cagtatgccc aggaaaaaaa tctcggcatg tggagcattg agctgccgtt
     3601 ttacggcccc gcgaaagtgg ttgcggcgca gtgggaacac gcgaaagaga aattgtccgc
     3661 gattcccagc gccaaattca cggaaggcac gacgcaccga ttcccgatca agcccgacga
     3721 gttgccgcaa ttcaattcgg ttgccgtggg catccccagt ctctccgtat tcggcgttgg
     3781 atcggcgcgc agcaaagggc acatctggtt ttcgccgatc attcccatga cgggcgaggc
     3841 aatactcgag gcgcaaaaag tgtttaccga cgcttacaag gaactgggaa tgtccatggg
     3901 gccaatgttt tttcttggtc cttcgtcgaa ttatccgcgg tgctttctgg tgcttttcgg
     3961 ttttccgatc gagcacgatg tcgagaa
//
LOCUS       DATNDT010000093         4408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3836656, whole genome shotgun sequence.
ACCESSION   DATNDT010000093 DATNDT010000000
VERSION     DATNDT010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4408
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836656"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="VN788_02755"
     CDS             <1..357
                     /locus_tag="VN788_02755"
                     /inference="COORDINATES: protein motif:HMM:NF020421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
                     /protein_id="HXU19483.1"
                     /translation="INYVMWRSQLCAAPDLWGLPYPGIQTGGSAVDSRELPNEGDSEQ
                     SVRRLKIEEHGDFFTGRVKPKIRLMGHWLERAGFKPGRHVSVRCLGSGVIELRSEGSK
                     MNEQNQASSDEVDCPS"
     gene            complement(429..1442)
                     /locus_tag="VN788_02760"
     CDS             complement(429..1442)
                     /locus_tag="VN788_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXU19484.1"
                     /translation="MTTSDFGTLLRNFFEQHLVAHRGLSTHTVLAYRDTWKLLLQFAS
                     RRHRKSCASLTLADLKADTVRRFLDHLEHERHNAVATRNNRLAAVHAFFQYVAQTEPR
                     ELAHCQSVLAVPFKRHAQRVPEYLERNEVLQIFSQINCQTALGRRDDSLLRLLYNTGM
                     RAQELVNLDVNHIRFSRPYMARILGKGRKERTCPLWTETIQALKTYLTARTVHLDEAV
                     PLFVNAQGNRITRFGLRHIITHRVAVAASVCPSLFTRKVTPHTWRHTTAMHLLQSNVD
                     LNMIRSWLGHASIETTNGYVEIDLEMKRKTLQSAEKLIPAKPSRKCSWRDDDKLMSWL
                     TKL"
     gene            complement(1429..2286)
                     /locus_tag="VN788_02765"
     CDS             complement(1429..2286)
                     /locus_tag="VN788_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXU19485.1"
                     /translation="MSELKPGQSISREVVERWNESIAHLSPNTRINRLCVLRRLCRYL
                     SYFDPDTCIIHSSFSPRRTRPAPHVYSRSDIRRIMAAARRLGPPGSLRPVVAANLIGL
                     LYATGLRIGEALNLTLQDMDLKRRVIEVREGKFHKSRYVPLSSSTTQQMKKYLRQRQR
                     AGFSTDPAAPVFLNIIGTRHGHPGFVTLFLELLRGLGLRGPKGQPGPRIHDLRHTFAV
                     HRLLAWYRQGGNLAAKLPLLSTYLGHSTITGTEIYLQATAQLLEQAGQRFHARFAVPF
                     TRGKSNDNF"
     gene            complement(2418..3674)
                     /locus_tag="VN788_02770"
     CDS             complement(2418..3674)
                     /locus_tag="VN788_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXU19486.1"
                     /translation="MLEYWFKEKRTLVDFRRGLLGVHFDGFAAYLQAKGYAHHTGCEL
                     LAKCCQFNAFLLEQRVERCDQICAARVEAFLKAYYAHLPEPVYGYSPRRTVRRAIKCL
                     FDYLIQIKVLTPPKPERIIKPFDWLLLPYLHHLRVDCHFSERTIKRCGVQVGALLEAM
                     GRNSQRRRLRALKAARIESIVKELLRTSTDPNGLTGTLRRFLRYCALRRHTRTDFSGL
                     VPPIRRYRHASLPKGLDDSVLEKMLKAIPRDTPVGARDYAIIVLMMAYGIRGVSAAQL
                     LLDDLDWQHSRIRIRAQKGGKEVVLPLMQAVGEALIQYLQHRPGQIPCRKLFLSAKAP
                     FRPLDSMAISMIVRRQLERAGVKTTGNGSRSLRHSWAIRALAHDSPIKSIADVLGHRY
                     IDTTFIYAKADLASLRQVALPWPTRN"
     gene            3765..4289
                     /locus_tag="VN788_02775"
     CDS             3765..4289
                     /locus_tag="VN788_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HXU19487.1"
                     /translation="MRRLRYHVAMSLDGFIAGPKGEYDWIVMDPTIDFTAFYSQFDTA
                     LMGRRTFELALTGPGAIMPGIQTIVCSRTLRGSDHQGVTIVSEATDAVSTLKSKPGKD
                     IWLFGGGTLFRGLLDAGLVDTIEVAVTPIMLSQGLSLLPAGRRSPPLELVDCKSKPNG
                     IITLSYQVGSVRSP"
BASE COUNT          963 a         1145 c         1339 g          961 t
ORIGIN      
        1 ataaattatg ttatgtggcg ctcccagtta tgtgcagcgc ccgatttgtg gggcttgcca
       61 taccccggca ttcagacagg agggagtgcc gtggattctc gtgagctacc gaacgagggc
      121 gattctgaac agtccgtccg ccggttgaag atcgaggagc atggtgattt cttcaccgga
      181 cgtgttaaac ccaagatacg ccttatggga cactggcttg aacgcgccgg ctttaagccg
      241 ggcaggcacg tttcggtcag atgcctcggt tcaggcgtca tcgagctgcg ctccgagggc
      301 tccaagatga atgagcaaaa ccaggcctca tccgacgaag tggattgtcc gtcctgatgc
      361 gggtctttcg ttcttttcac ttcacataac cgctgtgctc ttttccgggc gcagcggttt
      421 tcatgcggtt acagctttgt cagccacgac atgagtttgt cgtcatcccg ccaagaacat
      481 ttgcggctgg gcttggccgg gatgagtttt tccgccgact gaagtgtttt gcgcttcatc
      541 tccagatcga tctcgacata accgttcgtt gtttcgatgg aagcgtgccc cagccacgaa
      601 cggatcatgt tcagatcgac attggattgg agcagatgca tcgccgtggt gtggcgccag
      661 gtgtggggag ttaccttgcg ggtgaacaac gagggacaca cgctggcagc cacggcaact
      721 cgatgggtaa tgatgtgtcg caggccgaaa cgggtgatcc gattaccctg ggcgttgacg
      781 aaaaggggga cagcttcatc taggtgcacg gtgcgcgcgg tcaggtaggt cttaagggcc
      841 tggatggttt cggtccacaa cggacaggtt cgttctttcc ggcccttgcc gaggatgcgc
      901 gccatgtagg ggcggctgaa gcggatgtgg ttgacatcca gattcaccaa ctcttgggca
      961 cgcatgcccg tgttgtagag cagccggagc aagctgtcgt cgcgccgccc cagtgcggtc
     1021 tggcagttaa tttgcgaaaa gatttgcagc acttcattcc gctccaggta ttcgggcacg
     1081 cgctgggcgt ggcgcttgaa tggaactgcg agaacggatt ggcagtgggc cagctcgcgt
     1141 ggctcggtct gggcgacgta ttgaaagaag gcgtgaacgg ctgccagccg gttgttccgg
     1201 gtcgctaccg cgttgtgccg ttcatgctcc agatggtcca gaaagcggcg cactgtatcg
     1261 gccttgagat cggccaatgt caggctggcg caggatttgc ggtgacgtcg ggaggcaaat
     1321 tgcagcaaaa gtttccacgt atcgcgataa gccagcacag tgtgggtgct gagaccacgg
     1381 tgtgcgacga ggtgttgctc gaagaagttc cgtaacaaag tgccaaagtc agaagttgtc
     1441 attggatttg cctctggtga acggcacggc aaagcgggca tgaaaacgct gaccagcctg
     1501 ttccaggagt tgcgccgtgg cttgcaggta gatttcagtg ccggtgattg tgctgtgtcc
     1561 caaataagtc gagagcagtg ggagtttggc cgcaagattg ccgccctgcc gataccatgc
     1621 cagcaggcgg tggacggcga aggtgtggcg cagatcgtgg atgcgcggtc cgggttggcc
     1681 ctttggtcca cgcaggccaa ggccgcgaag gagttccaga aaaagcgtca cgaaaccagg
     1741 atgtccgtgc cgggttccaa tgatgttcag gaatacaggc gcagcagggt ccgttgaaaa
     1801 accggcgcgt tgccgctgcc gcagatactt tttcatctgc tgtgtcgtgg aggacgatag
     1861 aggcacgtaa cgggatttat ggaacttgcc ctcgcgaacc tcaatgaccc ggcgcttcag
     1921 gtccatatcc tgcaacgtca gattcaatgc ctcaccgatg cgaaggccgg tcgcgtagag
     1981 aagcccgatc aggtttgcag ctacgaccgg tcgcaagcta ccgggcgggc cgagtcgccg
     2041 agccgcggcc ataatgcggc ggatgtcact acgggagtag acgtgggggg ccggacgggt
     2101 ccgccggggc gagaagctgg agtggatgat gcaggtgtcc ggatcgaaat agctcaagta
     2161 acggcagagc cggcgaagaa cgcaaagccg gttgatccgg gtattgggac tcaggtgggc
     2221 tatgctttca ttccagcgtt ccaccacctc ccggctgatg gattgccctg gcttgagttc
     2281 actcatcaga aaacaatcca ggctgaggag gattttgtgg ctgctaagtc cacgttggcc
     2341 tcgcgcataa tgcgtctccc aaaaagtctg aagccggggc gccagcgcgc tgcggaaaaa
     2401 gcacgttgcc atacgcttta gtttctggtc ggccacggga gagccacttg gcgaagcgaa
     2461 gcgaggtctg ccttggcgta aatgaacgtt gtgtcgatgt agcgatgtcc cagcacatca
     2521 gcgatggatt tgatgggtga gtcatgggcc agcgcacgaa tggcccagga atggcgcaag
     2581 gagcgggagc cgttgccggt ggttttgacg cccgctcgtt cgagctggcg gcgaacgatc
     2641 atggagatgg ccatactgtc gagcggacga aacggcgctt tggcgctcag aaagagtttc
     2701 cgacacggga tctgtccggg acgatgctga aggtattgga tcagagcctc gccaaccgct
     2761 tgcatcaacg gcagcaccac ttccttgccg cccttttgtg cgcgaatacg aatgcgtgag
     2821 tgttgccagt ccaaatcatc caacagcagt tgggcggccg agacgccacg gatgccatag
     2881 gccatcatca gaacgatgat cgcgtagtcc ctggccccca ctggcgtgtc tcgcggaatc
     2941 gccttgagca ttttttccag cactgagtca tccaacccct tgggcaagga ggcatgccga
     3001 tagcgacgga ttggaggaac caagccggaa aaatcagtgc gggtgtgtcg tcgcaaagcg
     3061 cagtagcgaa ggaaccgacg caaagttccg gtaagaccat ttgggtctgt gctggtgcga
     3121 agcagctctt tcacaatgct ttcgattctc gccgccttga gcgcgcgcaa acgcctgcgc
     3181 tgactgtttc gccccatcgc ctcaaggagg gcgccgactt ggacgccaca gcgtttaatg
     3241 gtgcgctccg aaaagtggca atccacccgt agatgatgga ggtaaggcaa caggagccag
     3301 tcgaagggtt tgatgatccg ctccggcttg ggtggcgtga gcactttgat ctggatcagg
     3361 tagtcaaaaa gacacttgat cgcacgccgc acggttcgtc ttggggaata gccatatacc
     3421 ggttcaggta aatgagcgta gtaagctttg aggaaggctt cgacgcgagc cgcgcaaatc
     3481 tggtcacaac gctcgacacg ctgctccaac agaaaggcat tgaactggca acacttggcc
     3541 agcagttcgc atcccgtgtg gtgagcatag cctttggctt gcaaatatgc ggcgaagccg
     3601 tcgaaatgaa ctccgagcaa accgcgccgg aaatcgacca gggtccgctt ttctttgaac
     3661 cagtattcga gcataaaaag gcttgaaaaa gctgggtgga gtggaacact tttttgtttc
     3721 catcgaaaat gtttggcaat tctcgaatgc cgtctgcgaa gcctatgaga cgactaagat
     3781 atcacgtcgc aatgagcctc gacggcttta ttgcaggccc gaaaggtgaa tacgattgga
     3841 tcgtgatgga ccctacgatc gactttacgg ccttttactc gcaattcgat acagccctga
     3901 tggggcgtcg gacattcgag ctggcactca caggtccggg agccattatg ccggggattc
     3961 agacgatcgt ctgttccaga acattgcgtg ggtccgatca tcagggagtc acaatagtct
     4021 cggaagcaac ggacgccgtt tccactctca aatccaaacc tggaaaggat atctggctct
     4081 tcggaggagg aactctcttt cgaggtttac tcgacgcggg tttggtcgac acaatcgaag
     4141 tagccgtcac gccgattatg ctaagccaag gtctttcctt gttgccggca ggaagacgct
     4201 ctcctccctt ggaacttgtt gactgtaaat ccaagccaaa cggaattatt actctttcct
     4261 accaagtcgg ttcagttcgc tcgccctaat cgcccgatcc gtgcacggat ttatgtgaaa
     4321 aaaccggctc gatcacaaag atcaaaccgg cctgttctcg aagttttcgt tttcaactcc
     4381 acataaacgg atgcttcaca taacacaa
//
LOCUS       DATNDT010000094         2883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3221973, whole genome shotgun sequence.
ACCESSION   DATNDT010000094 DATNDT010000000
VERSION     DATNDT010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3221973"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..1307)
                     /gene="hisD"
                     /locus_tag="VN788_02780"
     CDS             complement(66..1307)
                     /gene="hisD"
                     /locus_tag="VN788_02780"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXU19488.1"
                     /translation="MRTLSGKAAAEQVRKLAMRASCFNEVEPKVQRIIDDVRTRGDAA
                     VREYSRQWDGLGEKDSLQVRESELREAWKGSSPKFRSSLKLAAANIRRFCQLQKPNEW
                     KKSYNGITLGQLVRPLDSVGCYIPGGRHPLVSTLLMTVVPAQVAGVKNIRVVSPRPSK
                     EVLAAAAMLGIRAFNQIGGAQAVAALAYGTSSIPRVDKIVGPGNVYVTAAKKLVSFDC
                     AIDMLAGPTEVVVVSHGGKPMYIASDLVAQAEHDPDAISIFITTSAKLAEAVSKSVDA
                     LAQGNDLAKQSLANSGVILIASSRRKAMDWANQIAPEHISVAKSDVSRVQNAGSVFVG
                     DYSPQAGGDYASGPNHVLPTAGVARFRGGLSVMDFVKIITVQELSKKGLRSIAPAVEF
                     LAETEGLKAHAESMRVRCGNA"
     gene            complement(1308..2177)
                     /gene="hisG"
                     /locus_tag="VN788_02785"
     CDS             complement(1308..2177)
                     /gene="hisG"
                     /locus_tag="VN788_02785"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HXU19489.1"
                     /translation="MKLRVGIPKGSLQEATLALFANAGLKIHTSSRSYFASTGDPEIE
                     CMLIRAQEMARYVEHGALDCGLTGQDWVIESGLEVQTVADLIYAKQSRNKVRWVLAVP
                     EDSRYQKAEDLGGCIIATELVSVTKNYFRDRNVDVKVEFSWGATEIKPPTLADAIVEI
                     TETGSSLRANHLRIIDTLLESNTQVIANKTSWQDAAKRRKIENLAMMLRGAMEAKERV
                     GLMLNVRKEDLNSVLAVLPSLKGPTISTLSDPNWLAVNTVIEEGTAWNVIPRLKEAKA
                     QGIVEYPLNKVVM"
     gene            complement(2174..2533)
                     /gene="hisI"
                     /locus_tag="VN788_02790"
     CDS             complement(2174..2533)
                     /gene="hisI"
                     /locus_tag="VN788_02790"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HXU19490.1"
                     /translation="MEPTIDFEKMGGLAPAMVQDADNGEILMLGFMNAESYAKTLSTG
                     YVTFFSRTRNKLWTKGETSGNRLKVLFAATDCDNDALLFKVHVEGDGLVCHEGTRSCF
                     TKNIAVAAAPVAQGAIR"
BASE COUNT          610 a          825 c          781 g          667 t
ORIGIN      
        1 gcagcccttc acgatttccc agcggcgggt ggtattcctt taggctgtga acggcgccac
       61 gtggattaag cattgccgca cctcacgcgc atggattcgg cgtgggcctt caggccctcg
      121 gtttccgcga gaaattcaac ggcaggcgcg atgctccgta gtcctttttt agaaagctcc
      181 tgcacggtga tgatttttac aaaatccatc acgctcaggc caccacgaaa acgcgccacg
      241 ccagcggtag gaagcacgtg gtttgggccg gaagcgtagt cgccgccggc ttgaggcgaa
      301 taatcgccga cgaaaaccga gcctgcgttc tgaacgcgcg acacatccga tttagcaacg
      361 ctgatatgtt ccggcgcaat ctggttggcc cagtccatag ctttccggcg agaggaggcg
      421 atgaggatca cgccgctatt cgcgagtgat tgtttcgcca gatcgttgcc ctgcgcaagc
      481 gcgtccacgg atttcgagac cgcctccgcc agctttgcgg atgtcgtgat gaaaatggaa
      541 atcgcatcgg gatcatgctc cgcctgcgcg acaagatcgg aggcaatgta cataggcttg
      601 ccgccgtggc ttacgaccac aacctctgta gggcccgcga gcatgtcaat cgcgcaatca
      661 aacgatacca atttcttcgc agcggtcacg taaacattcc ccggaccaac aatcttatct
      721 acgcgaggaa tgctcgatgt tccataggct agagccgcta cggcctgcgc cccgccaatt
      781 tggttgaacg cccgaatgcc gagcatcgcg gcggcggcaa gaacttcttt cgagggacga
      841 ggtgaaacca cgcgaatatt ctttacgcca gcgacttgcg cgggaacgac ggtcatcaga
      901 agggttgaaa ccagcggatg tcggccgccg ggaatatagc atccgacgga atcgagcgga
      961 cgaactagct gccccaaagt gattccgttg tacgacttct tccactcatt tggtttttgc
     1021 aattggcaga agcggcgaat attggcggcg gccagtttta aggaggaacg gaactttggc
     1081 gacgaccctt tccacgcctc ccgcagttcg ctctcccgca cttgcagcga atctttttct
     1141 ccaaggccat cccattgccg ggaatattcc cggacggcgg catccccacg agtgcgcacg
     1201 tcgtcgataa tgcgctgcac tttcggttct acttcattga agcacgaggc gcgcatggcg
     1261 agtttacgca cttgctccgc cgccgctttg cctgagagcg tccgcattta catcaccacc
     1321 ttgttcagcg gatactccac aatgccctgg gctttggctt ccttgagccg tggaattacg
     1381 ttccacgcgg ttccttcctc aatcaccgta tttacggcaa gccagttggg gtcgctcaag
     1441 gtggaaatcg tagggccttt cagtgaagga agcacggcga gaacagagtt aagatcttct
     1501 ttgcggacgt tcaacatcaa gccaacgcgt tctttcgctt ccatggctcc gcggagcatc
     1561 atggccagat tttcaatctt gcgtcgcttg gctgcatcct gccatgaagt tttattggcg
     1621 atgacctgcg tgttcgattc cagcagcgta tcaataattc gcaggtgatt ggcgcggagc
     1681 gagctgccgg tttcggtgat ttccacgatg gcgtcggcca gcgtcggtgg cttgatctcc
     1741 gtcgctcccc aggagaattc cacttttaca tctacattgc gatcgcggaa ataattcttg
     1801 gttacgctca ccagttcggt ggcgataatg caaccgccca ggtcctcggc tttttgatac
     1861 ctggagtctt ccgggactgc gagcacccag cgtactttgt ttcggctctg tttggcataa
     1921 atgagatcgg ccacagtctg tacttcaagg ccgctttcga tcacccagtc ctgccccgtc
     1981 aaaccgcaat ccagcgctcc atgttcgaca tagcgggcca tctcctgcgc gcggatcaac
     2041 atgcattcga tttccgggtc gccggtcgag gcgaaataag agcgcgaact ggtgtggatt
     2101 ttcaagccgg cgttcgcgaa gagcgcgagt gtggcctcct gcaagctgcc tttgggaatg
     2161 ccgacgcgga gcttcatcgg atggccccct gcgcgacggg cgcagccgca accgcgatat
     2221 ttttagtgaa acaagaccgc gtgccttcgt ggcagaccag cccatcgcct tcgacgtgca
     2281 ctttaaagag cagcgcgtcg ttgtcgcaat cggttgcggc gaagagcacc ttgagccggt
     2341 tgccggaggt ttcgcccttg gtccaaagct tgttgcgagt ccggctgaag aacgtcacat
     2401 accctgtgct taaagtcttg gcgtaagact cggcattcat aaagccaagc atcaggattt
     2461 cgccattgtc ggcgtcctgc accatggcgg gcgccagacc gcccattttc tcgaaatcaa
     2521 ttgtcggttc cattgctctt tccttaagtg tttcgttttt ccttcactac tttttaagta
     2581 ttgtcattcc gaaaccgctt caggctgtga ggaatctgct tgaaagcaaa aacagattcc
     2641 tcactacgct cggaatgaca attcgttatg cacaaacaaa aagcccgctg actctagcgt
     2701 cagcgggccc gaaactttgt gtgctggtta ctttcagcta caccgccgcc aacgcgccgt
     2761 gcgcgtgatg atggcgatga tgatgccgag tcagcatgaa gaaatagatt actggacagg
     2821 ctgaaagctg tcaacgaaaa ctggctttca gaaaaaaccg gccaggacgg aatcctggcc
     2881 ggc
//
LOCUS       DATNDT010000095         4928 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2950352, whole genome shotgun sequence.
ACCESSION   DATNDT010000095 DATNDT010000000
VERSION     DATNDT010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2950352"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="VN788_02795"
     CDS             <1..275
                     /locus_tag="VN788_02795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19491.1"
                     /translation="WVVFTWPHFRSVQLFGIPSGETHPVLLILVVLGFLANVCYCAAY
                     LVDIPLQLTFATWRRYRWALLLIGILFAFLLENYWIADEIYPFVTK"
     gene            742..1479
                     /locus_tag="VN788_02800"
     CDS             742..1479
                     /locus_tag="VN788_02800"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HXU19492.1"
                     /translation="MKMKILALLVMTAAAISLVAQTESPVQTPPAQTAPAAQTPATPQ
                     PARRPLSPSGIASAQVLGQWVKPNRQTYTMGGETYQGGKWIDITYGRPLMRGREAFTG
                     SGSDYGKSTIAQIPGGPNAPVWRAGANLSTRLKTEVPLVIGGATIPAGEYSLFIDLKQ
                     PTEWTFIVSSYGAATKIDPNNKDAIYGAFGYTPDKDVARAPMTVETLPYAVEELTWAF
                     VDMTADSGRIAIMWDKTIATVPFKVAQ"
     gene            complement(1583..2374)
                     /locus_tag="VN788_02805"
     CDS             complement(1583..2374)
                     /locus_tag="VN788_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19493.1"
                     /translation="MMLNAINFARSYGFEYVHTPFSSIGHTERPMEEWTAAWESFFNL
                     GSGETPCTEKRHEVVEFSHNFTELAKCFGCRWRWDEMAERFRAIIPEVRRKYYVNKSP
                     GKANQFTVAVHIRRGDVSQDNPGYFTADEAILRTIATTQSLLNARNVSYKIAVHSQGV
                     PADFANFSRLGVELFLNTDAVWTMRQLIQADVLIMAKGCFSYCAGLISHGIKIFEPDA
                     LPNHDVLPSWKWRAVPLADSWIPCKQDGSFDSSALERQLTLLTGR"
     gene            2914..4293
                     /locus_tag="VN788_02810"
     CDS             2914..4293
                     /locus_tag="VN788_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HXU19494.1"
                     /translation="MALLRKLIAAGIVLTFAASMMFAGKGEAAPKSLLAATPPMGWNS
                     WDSYGLRITESEFRDNVAVLASKLKPFGYTYAVIDEGWFLVNPEDRPHPERLKYEIDA
                     NGRYIPVAARFPSAMQSDRNSGFTALAGWVHSQGLKFGIHIVRGIPRESVQRNLPIEG
                     SSFSAEDAADTSDACPWDPTNWGVKDNAAGQAWYDSLLRQYASWGVDFLKVDCIANNP
                     YKASEIRQIHLAIQHSGRPIVLSLSPGPTALEHAAEVGSLSQMWRISNDIWDVWSSNR
                     EFPRSVKSQFDLAAQWVKFARPGNWPDADMLPVGELRPYPDVGPGARHTRLTADEQRT
                     QISLWAFARSPLIVGANLTALDSETLQLLTNGDILRIDQSALASRQALHEGDVYVWTA
                     DFPRGEQAVGVFNTGDTTVSFDRALPALGLGNKRVSLRNVWEGSVQKHASSLQAQLPP
                     HGVVVDWVH"
     gene            4401..>4928
                     /locus_tag="VN788_02815"
     CDS             4401..>4928
                     /locus_tag="VN788_02815"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HXU19495.1"
                     /translation="MRNAASRIALAASVLTVAVITMCAALQTTAAPAQPQTPPSVTQA
                     AGRGRGNFREPDPIDFDDRAGYTEMFDGATLNGWDGNPDMWKVVDGAIVGERPAPASG
                     MTASPFRQTFLVWQGGQPADFELKLEIKLEGPNADSGIQYRSFIAPLNAGRAGAPPLD
                     PREAKWNLAGYQLDFN"
BASE COUNT         1081 a         1324 c         1437 g         1086 t
ORIGIN      
        1 cgtgggtggt gttcacatgg ccgcactttc gttctgtgca attattcggc atcccttccg
       61 gtgagactca tcccgtcctc ttaattcttg tcgttctcgg gtttctggcc aatgtctgct
      121 actgcgcagc gtaccttgta gatatcccgc tccaactcac attcgctaca tggcgcaggt
      181 atcgctgggc cctgctgctt atagggatat tgttcgcttt cctgctcgag aattactgga
      241 ttgccgacga aatctacccg ttcgtaacaa aataaaatct ggcaccggtt gcgtcttgcc
      301 gaacacgagg cagagtgcgg aagcggcatc gtcccttacc gcacatgcga tagccctgca
      361 cggacggcgc ggcgagatgt tggagccgtg gaccattgtg gtttcggctt cgaagcctct
      421 gcagcaggct cggtggcgaa agaccgggag gccagcactc tatgtgcggt gacgcgcgtt
      481 aattccgtgc tcgcaacgca agagtcactc gcagcacgac ccggtgcgga tgcttcttga
      541 ctttcgatgg aacgggcatc cgccgctgca ccttgtagtt tccgcgtcgg attgaacgct
      601 aaattcgtgt aacattgcgc cactacaagg cgtcactaca tgacgccacc acacgcgtgg
      661 cgggcgctga agttgggctt caacattgag cttcaatatt aagcttcgat gaagcttcaa
      721 cttcgagatc gggaggattc gatgaaaatg aagattttgg ccttactcgt gatgactgcg
      781 gctgccatat cgctggtggc tcagacggag tcgcctgttc agacgcctcc tgcgcagaca
      841 gctcctgccg ctcaaacgcc ggctactcct caaccggctc gacggccact tagtccgtcg
      901 ggaatcgctt cggcgcaagt cctcggccaa tgggtgaagc caaaccgcca gacgtacacg
      961 atgggcggcg agacctatca aggcggcaag tggattgaca ttacgtacgg gcggccgttg
     1021 atgcgcggcc gagaggcgtt taccggaagc ggcagcgatt acgggaagtc gaccatcgcg
     1081 caaatcccgg gcgggccaaa tgcgccggta tggcgcgccg gggcgaacct ctcaacgcgc
     1141 ctcaaaaccg aggttccgct ggtgatcggt ggtgcgacga ttccggcagg agagtacagc
     1201 ttgtttatcg atttgaagca gccgacggag tggacgttca tcgtctccag ctatggagcg
     1261 gcgacgaaaa tcgatcccaa caataaggat gcgatctacg gcgcgtttgg ctacacgccg
     1321 gacaaagacg tggctcgcgc gccgatgaca gtggagacgc taccatacgc ggttgaggaa
     1381 ctgacttggg cgtttgtgga tatgaccgcc gacagcggac gcatcgcaat catgtgggac
     1441 aaaacgatcg ctacggtgcc attcaaggtc gcgcaataaa atggcggagt ggctcgtaag
     1501 tctgactata gattacggac gctttgatcg aatgcgccgc gcgccaggca cgctttgagc
     1561 ggatggccat cgtcatcctc tcttagcgtc cggtcagaag agtgagctga cgttcgagcg
     1621 ctgaggaatc aaaagagcca tcttgtttgc acggaatcca actatcagcg agaggaacag
     1681 cacgccattt ccacgatggt aagacatcat ggttgggcaa tgcgtccggc tcaaaaatct
     1741 taatgccatg ggaaatgagc ccggcgcagt agctgaaaca acctttggcc atgatgagaa
     1801 cgtcggcttg tatgagctgc ctcatcgtcc aaacagcatc ggtattcaga aatagctcaa
     1861 cgccaagacg agaaaagttg gcaaagtccg ccgggacgcc ctgagagtga acagctattt
     1921 tgtaggaaac gttgcgggcg ttaagtaggg actgcgtcgt ggcgatcgtt cgtagaattg
     1981 cttcatcggc ggtgaaataa cccggattgt cttgcgacac atcaccccga cggatgtgca
     2041 cggcaactgt aaattgattc gcctttccgg gagacttatt cacatagtac ttacgacgga
     2101 cctcgggtat tatggccctg aatcgttcgg ccatttcgtc ccaccgccac ctacaaccaa
     2161 aacacttagc aagctccgta aaattgtggg aaaactcgac cacctcatgt cgtttctcag
     2221 tgcaaggcgt ctcccccgac ccgagattga agaatgattc ccaggctgca gtccactctt
     2281 ccattggtcg ttcagtatgc ccaatagaac tgaacggtgt atgaacgtac tcaaaaccgt
     2341 atgaacgagc aaaattgata gcattcagca tcatcagcgc ctgcgaacca gctccttcgc
     2401 tcggaaggcc cctgcatgta atctttcgga tcggtcgaat tccagtcaaa tgaagtaggt
     2461 actgccgcag aaacaaaccg gtctcgtcga aagtggtgtt ccggcccacg atgaatttgg
     2521 cgcagttttt tagcttacgc cacccgcttt tgccagacgc actcaacaga cacagggcta
     2581 tatctcggcc ttgcagtagt gtgcggcgtg atgaatgaga acttgcggtt tgatcggggt
     2641 gttgctgggg cggatgcatt gtcttaggcc gtaactcctt ctcgatcaaa atgtcatact
     2701 ccgcccaccg cggacaaaac atttgtctct gcaagtccgt atattcggcc aggcgagaca
     2761 tccatgggcg atgctactaa ctacatgtcc gcgtcgaggg gcttagatta attcgattgc
     2821 gtgttcgcaa ggcggatatt tctgcgatac tctcagcgga attttaacta tgactcgtac
     2881 ctacgattta cgttcgctat gaggccgaca aagatggcgt tgctgaggaa gctgatcgct
     2941 gcggggatag tgctgacttt cgcagcgagc atgatgttcg cgggaaaggg cgaagctgcc
     3001 cctaagtcct tgctggctgc cactccgcca atgggttgga acagttggga ctcttacggg
     3061 ctccgcatta cggaaagcga gttccgcgat aacgtcgccg tgctggcgtc caaactgaag
     3121 ccgttcggat atacgtatgc ggtgatcgat gagggctggt tcctggtgaa tcccgaagat
     3181 cggccgcatc ctgagcgctt gaaatacgaa atagacgcga acggcagata tattcccgtg
     3241 gccgcacgct ttccatcagc gatgcaaagc gaccggaaca gcggctttac ggcgttggcg
     3301 gggtgggtgc actcgcaggg attgaaattc ggaatccata ttgtgagggg cattccgcgc
     3361 gagagcgtgc aacgaaatct tccgatagag gggtcgtcgt tctctgccga agacgccgcg
     3421 gacacaagcg acgcttgtcc gtgggatcca acaaactggg gcgtgaaaga taacgccgcc
     3481 ggtcaggcat ggtatgactc tttgctaagg caatatgcgt cgtggggcgt ggatttcctt
     3541 aaggtggatt gcattgccaa caacccttac aaagcgagcg agatccgcca gattcatctc
     3601 gccatccaac acagcggaag gccgattgtc ctgagcttgt caccggggcc gactgctctg
     3661 gaacacgccg cggaagtagg ttcgctctcg cagatgtggc gcatctcgaa cgatatttgg
     3721 gatgtgtgga gcagtaacag ggagttccct agaagcgtca aaagccagtt tgatctcgct
     3781 gcgcagtggg tgaaattcgc gcggcccggt aactggcccg acgccgatat gcttcccgta
     3841 ggcgaactgc gtccttatcc cgacgtgggg ccgggggcgc gccatacgcg gcttactgcg
     3901 gatgagcagc ggacgcaaat cagcctgtgg gcgttcgcgc gtagcccgtt aattgtaggc
     3961 gcgaatctta ccgcgctaga tagtgagacg ctgcaacttc ttacgaatgg ggatattctt
     4021 cgcatcgacc aatctgcgct tgcgtcacgg caggcacttc acgaaggcga cgtgtacgtc
     4081 tggacggcgg actttcctcg tggagagcag gcggttggcg tcttcaacac cggcgataca
     4141 accgtgtcgt tcgatagagc attgcccgcg cttggcctag gtaacaagag agtcagtctt
     4201 cggaatgttt gggagggcag cgtgcagaag cacgcttcat ctctgcaggc gcaattgcct
     4261 cctcacggcg tggtcgtgga ttgggtgcac tgaaccgtgg cgttttggcg aggatcgtgg
     4321 taattttcgc ggccaacttg cgagcccatc agtagcgaag ttaacgtctt ttcaaaagtc
     4381 ggcgggcccg gagggattcc atgcgaaatg ccgcttctag aattgcgctc gctgcttcgg
     4441 tgcttaccgt cgcggtgata actatgtgtg ccgcgttgca aacgacagcc gcgcctgcgc
     4501 agcctcagac tccgccctca gtaacgcagg cggctggccg tggtcgcgga aatttccggg
     4561 agcccgatcc catcgatttt gacgaccgcg ccggttacac cgaaatgttc gacggcgcca
     4621 cgctgaacgg atgggacgga aatcctgaca tgtggaaagt ggtcgatggc gcgattgtcg
     4681 gcgagcggcc ggcaccggcg tccggcatga ctgccagccc gtttcgtcag acatttctcg
     4741 tttggcaagg tgggcagcca gccgattttg aattgaagct ggaaatcaaa cttgaaggac
     4801 ccaatgccga tagcggcatt caatatcgca gcttcattgc gcctttgaac gcggggcgcg
     4861 ccggtgctcc gccgctcgat ccgcgcgaag caaagtggaa tcttgccggg tatcagctcg
     4921 acttcaat
//
LOCUS       DATNDT010000096         3665 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3576470, whole genome shotgun sequence.
ACCESSION   DATNDT010000096 DATNDT010000000
VERSION     DATNDT010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3665
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3576470"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..526
                     /locus_tag="VN788_02820"
     CDS             <1..526
                     /locus_tag="VN788_02820"
                     /inference="COORDINATES: protein motif:HMM:NF021126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HXU19496.1"
                     /translation="VNDPGAIYTDANWKVYENSAAFPRAWIVHESAVERSRDKLLHEL
                     ASPATQLDRVALVPEPLTEALEPLSQSGSELATVERHGINGPQIKVHAQSRALLVTSE
                     IFYPGWHATVNGRAARIHEVDGALRGVVVPKGESDVVFHYAPRPVFFGATLTGLAFFG
                     VGIMAFLDRKRSRS"
     gene            complement(521..2029)
                     /locus_tag="VN788_02825"
     CDS             complement(521..2029)
                     /locus_tag="VN788_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 39"
                     /protein_id="HXU19497.1"
                     /translation="MSTRRGRSLRYLIVGFALLLAPGLRAQEQVEINTRAQTSAFPHF
                     WEQMFGSGRAILSLRESYRNDLRAVKQVTDFRYVRFHAIFHDETGVYNEDERGNPVYN
                     FAYIDEIYDGLLKNGVRPVVEIGFMPKKLAFNPDALHPFWYKQNVSPPKSWERWDGLI
                     EHFARHLLERYGVDEVAQWYCEVWNEPNIDFWNGIPRQKSYFELYDHTARDLKRVNPR
                     LRVGGPATAAAAWVGDFLKHAAEDHVPVDFVSSHGYADDTVENLFGTHENIPMDDRVC
                     RAIAKVRGQIHSSAMPELPLFWTEWNVPGMDGARDTSYVGPALANTIRECDGMVDMMS
                     FWTFSDVFEEGGPIPQPFEGHFGLRAKGGINKPSYYDFGLLHKLGERRIANAASDVIV
                     TKRKDGTLVIAVWNLVDPDKTGSTKTVRLIFDGLRNDAKATVSRVDDEHGNTLAAYKA
                     MGSPRYPTEDQIRRLNTTTALPMPTRRQLEGNRLDLTLEPNALVIVEIEGGL"
     gene            complement(1993..2979)
                     /locus_tag="VN788_02830"
     CDS             complement(1993..2979)
                     /locus_tag="VN788_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HXU19498.1"
                     /translation="MKQFQMRHSGSVGVNAAISKGLKFLLARQSAQGFWADWQLPPGE
                     SLIWTTAYVGYRLSSLCETHRNSAEAAMQKAARWLLIRELPGSGWGYTEEVPCDADST
                     ALAILFLTSQGFSIPERTWERLREFQRPDGGFATFTAHAGVGSWMLSHPEVTATALMA
                     LIPENPSSGPSIERGIAYSRKQMTSAGLWNSFWWKSSLYATESNLLLFRRAGFESGLT
                     RTALRTIRPENSFEHALLILSLLHAGNAINAPELNESVEALLSAQLQDGSWPSVPILR
                     LTDRDCSEPWTRNDAGPLFADQNRIFTSATVVAALCNKESLCQHAVAGHCVI"
     gene            complement(2976..>3665)
                     /locus_tag="VN788_02835"
     CDS             complement(2976..>3665)
                     /locus_tag="VN788_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19499.1"
                     /translation="GPGIYWIGVSFPIAGAPALTVYINSRLSAELGQWERMSEFTRYF
                     RGMSLGGLDAWRHVEPELRGRMSPLGMAVTISPSRPARGRVYFSAYGLGFDYYRGLLL
                     KASDSEDCATLYDRYIEAMVREDRRYPARSAVWSIEFESGRSPNIKFELCAHCAFSSD
                     AEAASRCESWLVSQGLDTKLYWDTIKLLTAGSRLSNTTPPELHAYAGVGQRSEESYAT
                     FYLNPGPFLAG"
BASE COUNT          776 a         1084 c         1021 g          784 t
ORIGIN      
        1 ggttaacgac ccaggagcga tctatactga cgcgaactgg aaggtctacg aaaactccgc
       61 tgcattcccg cgcgcctgga ttgtgcatga gagcgccgtg gaacgctcgc gagacaagct
      121 gctacatgag ttagcatcgc ccgccaccca acttgatcgc gtggcgttgg ttcccgagcc
      181 gctcaccgaa gcgctggaac cactatccca gagcgggtct gaactggcca cggtcgaacg
      241 gcacggaatc aatggaccgc aaatcaaggt tcatgcgcag agccgggcac tgttggtgac
      301 gagtgagatt ttctatcccg gatggcacgc cacggtgaat gggcgggcgg cgcggatcca
      361 tgaagtggac ggcgctctgc gtggtgtcgt tgtgcccaag ggcgagagcg acgtcgtttt
      421 ccactatgcc ccgcggccgg tattcttcgg cgcgacgctc accgggctcg catttttcgg
      481 ggtagggatc atggcttttc tggaccgtaa acgttcacga tcataaaccg ccttcgatct
      541 cgacgatcac cagcgcgttt ggttccagag tcagatcgag acgatttcct tccaactggc
      601 gccgggtggg catcggaagc gccgttgtgg tatttaatcg gcgaatctga tcttcggttg
      661 gatagcgggg gcttcccatg gctttgtacg ccgccagcgt gtttccatgt tcgtcgtcga
      721 cgcggctcac cgtggccttc gcgtcattcc gaaggccgtc aaaaatcagt ctcaccgttt
      781 ttgtactccc ggttttgtca gggtcgacta agttccaaac cgcgattact agagtcccgt
      841 ccttgcgctt tgtgactatt acgtccgaag cggcgttcgc gatacgccgc tcgccgagct
      901 tatggagaag accgaagtcg tagtaactcg gtttattgat gccgcctttg gctcttaatc
      961 caaagtgccc ctcgaacggc tgcggaatcg ggccgccctc ttcgaatacg tcggaaaagg
     1021 tccagaaaga catcatgtcc accatgccgt cacactctcg aatggtgttc gcaagcgccg
     1081 ggccaacgta agaagtgtcg cgcgcgccat ccatgccagg aacgttccat tccgtccaaa
     1141 atagcggtaa ttccggcatt gcagagctat ggatttgtcc tctaactttg gcgattgccc
     1201 ggcacacgcg atcatccatg ggaatatttt cgtgcgttcc aaaaaggttc tcgacggtat
     1261 cgtccgcgta gccatgagat gaaacgaaat ccactgggac atggtcctcc gccgcgtgtt
     1321 tgagaaaatc gcccacccag gcagcggcgg ccgtggccgg accgcccacg cgcaacctgg
     1381 gattaacgcg cttgagatcc cgggcggtat ggtcgtaaag ctcgaagtaa ctcttctggc
     1441 gagggatgcc attccagaag tcgatattcg gctcgttcca cacttcgcaa taccattgtg
     1501 cgacttcatc cacgccgtag cgttcgagca agtgccgcgc aaaatgctca atcaggccgt
     1561 cccatctctc ccagctcttc ggtggcgaga cgttctgctt gtaccagaaa gggtgcaacg
     1621 cgtcgggatt gaatgccagc ttttttggca tgaagcctat ttccacgacc ggccgcacgc
     1681 cgttttttag caggccgtcg tagatctcgt cgatataggc gaagttgtaa accggattgc
     1741 cgcgttcgtc ctcgttgtac acgccggtct catcgtggaa gatggcgtga aagcgtacat
     1801 agcgaaaatc cgtgacttgt ttcaccgcgc gtaaatcgtt gcgatagctc tcccgcagtg
     1861 acaggatggc gcgcccggag ccgaacatct gttcccagaa atgagggaat gcggaagttt
     1921 gcgcgcgtgt gttgatctcc acttgctctt gagcgcgcag tcccggcgcg agtaacagcg
     1981 caaatccaac gatcaaataa cgcaatgacc ggccacggcg tgttgacata gactttcttt
     2041 attgcatagc gccgccacga ccgtcgccga agtgaagatc cggttctggt ccgcgaatag
     2101 agggcctgcg tcatttcgcg tccacggctc cgagcaatcg cgatcagtca gacgcaggat
     2161 cggcacgcta ggccaactcc catcctgcag ttgggccgac agcagcgcct ccaccgattc
     2221 attaagctca ggcgcattga tagcgtttcc ggcatgtagc agactcagga tcagcaaggc
     2281 atgctcgaag gagttctccg gccggatggt acgaagcgcc gttcgagtta ggccactttc
     2341 aaagcctgcg cgccggaata agagtaagtt cgattcggtt gcataaagag acgacttcca
     2401 ccagaaggaa ttccacaaac ccgccgacgt catttgtttg cgtgagtatg caatgccgcg
     2461 ctcgattgac ggtccggacg atggattttc gggtattagc gccatcagcg ccgttgcagt
     2521 aacttccggg tgcgataaca tccacgagcc cacgcccgcg tgcgcggtga acgtcgcgaa
     2581 tcctccatcg gggcgctgga attcccgcag gcgctcccag gtccgttccg ggatggaaaa
     2641 cccctgcgat gtcaggaaga gaatcgcgag cgcagttgaa tcggcatcgc acgggacttc
     2701 ctccgtataa ccccaaccgc tgccaggaag ctcgcgtatc agcagccagc gggcggcctt
     2761 ttgcatcgcg gcttccgcgc tattccggtg ggtttcgcac aaagaggaga gccggtatcc
     2821 aacgtacgcg gtcgtccaga tgagagactc tcccggcggt agttgccaat cggcccagaa
     2881 gccctgcgcc gattgccgcg caagcaagaa cttcaagcct tttgaaatcg ctgcattgac
     2941 accaacgctg ccggaatgcc tcatctggaa ctgcttcatc ccgccagaaa cggtccagga
     3001 ttcagataga acgttgcgta tgactcttcg gaccgttgcc caacgcccgc gtaagcatgc
     3061 agttcaggcg gcgttgtatt actaagccgg ctccccgcgg ttagtaactt aatcgtatcc
     3121 cagtacagct tcgtatcaag cccctggctc acgagccagc tctcacatcg actggcggcc
     3181 tccgcatcgg atgaaaaagc gcaatgcgcg caaagctcaa acttgatatt cggactccgg
     3241 ccggactcaa actcgatcga ccagaccgcc gaccgcgccg ggtatcgccg gtcctctcta
     3301 accatcgctt cgatatagcg atcgtataat gtagcgcagt cctcgctatc gctggcctta
     3361 agcagcaacc ctctgtagta atcgaagccg agaccatagg cgctgaaata gactcttccg
     3421 cgggccgggc ggcttggcga aatagttact gccattccaa ggggcgacat tcggccgcgc
     3481 aattccggct ccacgtgccg ccatgcgtct aatcctccta agctcattcc tcgaaagtaa
     3541 cgcgtgaatt cgctcatgcg ttcccattgg ccgagttcgg cgctcaatcg ggaattgatg
     3601 tagaccgtaa gagccggcgc gcccgcaatc gggaagctga cgccgatcca gtaaattccc
     3661 gggcc
//
LOCUS       DATNDT010000097        18808 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2783576, whole genome shotgun sequence.
ACCESSION   DATNDT010000097 DATNDT010000000
VERSION     DATNDT010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 18808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18808
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2783576"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="VN788_02840"
     CDS             <1..630
                     /locus_tag="VN788_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19500.1"
                     /translation="RLEAYTFFTFAEDTARSPWEPYHVSLGYKPEDSTVTVATSGTAP
                     LVFGGGAVMPWTGQGVIDSIVAQIKSRGVVWPHSQTYILALHPECAVDLATRGYTRKS
                     LQEYLFERTRVSYEKLAGPVFFGHDNEIDFIRTSIADGRIRPDRAPLFLEALKPGGFV
                     PVVQSPADFHIVVAGGSPGYDLLFSYPGTNWANQTKRISAAMLTTAGRG"
     gene            complement(717..1055)
                     /locus_tag="VN788_02845"
     CDS             complement(717..1055)
                     /locus_tag="VN788_02845"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HXU19501.1"
                     /translation="MLTFIDTNKLPRKKTAEGEVTEVLNRELCGAKNVVAALRWLKPG
                     ERFEAKASDKYQLLYLMEGSGRIRLDDKDYEVLKGAGVYLGPSESATIQPAEGASLKL
                     LHLVVPQIPA"
     gene            complement(1083..1499)
                     /locus_tag="VN788_02850"
     CDS             complement(1083..1499)
                     /locus_tag="VN788_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU19502.1"
                     /translation="MAYIFHENDLPRLISAVPGRERTFFVNKELTKIDDMLAGVMHYV
                     KGASSPYHLHKNCEHFYFIMEGQGTVESEQETRDVGPGDLVFIPAEAKHRLRASKTPL
                     FYFEFQAPNRFVTTILDGTPDDLRWNRVEGGVWVQT"
     gene            complement(1527..2897)
                     /locus_tag="VN788_02855"
     CDS             complement(1527..2897)
                     /locus_tag="VN788_02855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HXU19503.1"
                     /translation="MTYRSLEDVLQTVGSPVKLLRNSQIGPYAFPVVRPEFTNWRDEQ
                     RAWKESCALFDQSHHMTDLYVEGPDALKLFSDLAINSFKNFKVNQAKQFVACNHDGYV
                     IGDAILFYLNENRFNLVGRPPAHNWVQYNVETGKYRASAERDERSAVNQGQRKAFRYQ
                     IQGPNALRVMEKVTGKPTPDIRFFNIDVLTIAGRRVSALRHGMVGQPGWELYGPWEYG
                     DEVRNAIVEGGQEFGIRQVGSRAYPTTCLESGWIPSPLPAIYSGEEMKAYRQWLKPTS
                     YEATASLGGSFYASDIRDYYLTPYDLGYGPFVKFDHDFVGKQALEKMVNDQKRKKVTL
                     VWNKDDIKRVTGSLYEGGDIFKYIDTPLANYSSLPFDKVVKGGKTIGVSTYTGYTYNE
                     RVMISLAVVDNQHSEIGTEVTVVWGEENRGSPKPTVERHRQAEIRATVAAVPYAEVAR
                     VAYRPH"
     gene            3047..3964
                     /locus_tag="VN788_02860"
     CDS             3047..3964
                     /locus_tag="VN788_02860"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXU19504.1"
                     /translation="MELRHLRYFLAVADALNFSRAAERLHLSQPPLSQQVRRLEAELG
                     AKLFDRSKRQIKLTAAGEIFATKARAILAQCDEAAKLAARAERGEFGHLRLGLWQLPV
                     PPVQTRLIIECLRAFVKRSPDVHLTLQSLLPAEQIKAVKDGGIDVGIVRLPVAQKSRG
                     IEIETILREPMVLAMSENNSLARFKTVPLRELSNQRYIMFGRDANADFHHFIARACRK
                     EGFSLNIAYFQDDLYSVWALIGAGLGVALAPASLQELRLPGIVFRKFEPAAPVMEMAL
                     AHMHTDKVSVVSQFLEVARKIAKAYSGGH"
     gene            complement(4023..5651)
                     /locus_tag="VN788_02865"
     CDS             complement(4023..5651)
                     /locus_tag="VN788_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HXU19505.1"
                     /translation="MDRRSFLSYGTAATASLFARGAFHQALAQDLGCSTPSAVVRTTA
                     GRVRGITRDKVSAFFGIPYGASTADARRFMPPSKPDSWTGVRDALEFGPRSPQGPSGL
                     IPEVAAVDRREPASEDCLRLNVWSPGLGKGNRPVMVWLHGGGYSQGSGAFIIYDGANL
                     ACRHDVVVVTVNHRLNVFGYLYLAELGGEKYANSSNVGMLDIVAALEWVRDNISAFGG
                     DPSNVTIFGQSGGGGKVSTLMAMPSARGLFHRAIVESASAIRGVPKDEATKSAEAYLG
                     RLGVKSNQIDDVQKMPAEQLLAAMGTGGAAGGGGLRLAPVVDGHSLPRNPFDPDAPEI
                     SATVPLLIGSVETEVTFMRGTPVDPMDDAALHMHVKQALRGAADDSVDRLIEVYRKGR
                     PNISNTDLYLVLASDATFRAGVLTEAERKADQGMAPVYMYYFTWKSPVREGKLRTFHT
                     LEIPFVFDNVDLATAMTGSGQDRYALEDKISSAWVAFARTGNPSHKGIPNWPAFNTKQ
                     RATMILNNECEVVDDPNGEERQALEAIRHAGAGA"
     gene            6222..9938
                     /locus_tag="VN788_02870"
     CDS             6222..9938
                     /locus_tag="VN788_02870"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU19506.1"
                     /translation="MLALGLGVLLLCIPAFSQLNLGSISGTVTDTSGAVITGATVTVT
                     DVERGVSRTLTTDTAGQYSAPSLTPGSYSVRVEFAGFQTLNRTGIAVGVGQAVRVDLA
                     MQPGAQTQTVTVTEAVPLINTANEVLSATVEAPQLAELPINGQLYTKVLEFQPGIHGN
                     PGGNSPNYSTNGASTQGNYFMLDGVENSNIFVNSGPLIGAATSTDELTILPQDAVEEV
                     NVMTNPPAEFGWFQGAVVNVGLKSGTNALHGTAYGYGRNNNLDAYDPYLNGAPKQDDN
                     FKQFGGSLGGPIKKDRLFFFGAYDGMRYKVGTAGTATVPTVNGGAVDAPSNSVPLAID
                     DMINNHGVLPNQLSLNLSDCTVAGSAPTAVATCGTKGVWRNNSLQTIGIGVPGDSFGH
                     SNNVIGRVDFHPNDRNSINGEYFFGQAVTSTPNVGEPSFWNNLNLSRTQMMRAVWTYT
                     PSSNWVNDVRFGYNRYNLADGNAECTGNGNFSSGNVGQPNYAALGFVSGANPPAPFCG
                     FPAVNFNQSGFPSFGALSFFISDQGVFQYTYTAYDNASYTHGSHNFHFGFEFHHSRFH
                     GIGAPGFLDGVLNFDGGVAFGPCGAVGEPACPAGAAAGENLASTDLQDFLAGSLGANN
                     QILVNPQQEDTGVGFNREALYFSDDFRASSRVTVNLGLRYELEPAIKVDNNTAGNFSA
                     SSPTGMVQQNGSAVYQSFHKAFAPRAGFAWDITGKGTTVLRAGMGVSWDTPQVDDLVA
                     QSFGAGINAIPTGFSLFNDAGLVFPASTNPQAVKTAVVSIPGPGLTWALNTPVFGAGL
                     NSDLACGTGNPAIVLPDGSNPSPCTLKARGTVVRLPGGGLSMNTQNARSPMYTWTLGI
                     QHAFGARTSLTVNYVGTHTYDLASEININQAAPGATGAGLLPPGVGLGTVDGPLQQRQ
                     PYFGQFPWFNGIFVYGPAGFSNYHALQVSFVQRSFHGLTLNAAYTFSRDLATPKGGNN
                     PYIDNSQCVSCDYGLQTPTQDLGVTLVYAVPGFKAPAQMLEGWEISSAIDVQSGDPFT
                     GLDGSDDFAGVGNGRNLFGGASEPWSLFGKGTNFKGIGKLAPVPCFGSSFVFAVLGGC
                     NPVLPAACISAANAEPVNAAMNAQIPGSSSGLASLMSAGCYMSPNGKSVIIPPAQGTF
                     GNMRPGALIGPPFHEWDLSLRKAWKIHERLNLQASLSAYNVLNNHAYAGGFTGGIVNL
                     PFLFGQSSGQPNDGNPINGTGGARIVSVGLKATF"
     gene            complement(10463..11080)
                     /locus_tag="VN788_02875"
     CDS             complement(10463..11080)
                     /locus_tag="VN788_02875"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXU19507.1"
                     /translation="MQIDAFLKVIEATRFATTIRDSIWMFPIIESIHVMSFTLVVGTI
                     AIIDLRLLGLASTQRSFQRISSDILKWTWAAFVLAVATGVMMFTTNARVYYHNPFFRT
                     KMLLLVLAGVNMAVFEITGRRTVHRWDRDPSPPRAGKVAAILSLTLWISIIFMGRLIG
                     FTTHPGKVLPPPPGVNFDNFLQQAPTGNGGAAPAPAKPPANQPQR"
     gene            complement(11102..11623)
                     /locus_tag="VN788_02880"
     CDS             complement(11102..11623)
                     /locus_tag="VN788_02880"
                     /inference="COORDINATES: protein motif:HMM:NF042488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6644 family protein"
                     /protein_id="HXU19508.1"
                     /translation="MSRFAEWLTKTSLSHTFRVQEGWLIPGVQSIHIIGIAVIIGSVF
                     MIVLRILGWAGTDQTLRQTTDRFGPWLIGGLCLQLVTGIVMIIAEPARELVNFSFWTK
                     MVFVAIATVLSGIFLVRVPRHEQRWETLVTHRSIKSLAILTFLIFLFIIVLGRLIAYD
                     HIWGSWSPATQEY"
     gene            complement(11666..12136)
                     /locus_tag="VN788_02885"
     CDS             complement(11666..12136)
                     /locus_tag="VN788_02885"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXU19509.1"
                     /translation="MKKTLITSCVIAACVAFLSLGIYVKPAVAHHSFAMYDQTKVLVL
                     TGVAHQFIAQANHAELHFYLIGPDGKLEKDKNGQLIDWGVEMAGAAAVAQQGITADSF
                     KAGTIFTVHMNPLRTGGNFGSRVGPIYKCPWRTPPAAGKTCDTVPGMTTISGRF"
     gene            complement(12205..13602)
                     /locus_tag="VN788_02890"
     CDS             complement(12205..13602)
                     /locus_tag="VN788_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19510.1"
                     /translation="MNSRRLVVVFGFMALIMLTWMCPVSSAQDQQGPQAKQDPQAEQQ
                     CAKAQGTRGQFRGRGNALSGPHGPYTNVIGPLSICTPTFDGPPAGMKALPIDLFKSKN
                     FYLDKKYWSDPRYFRCNTPRQLTDMWTSRRFFGPGKAPENAAWGDCKWDIPRDQLVSH
                     YPYKTAKEQYDALLAAAKAKGGPTVYTKATTPDWDGWYTRDMTTDTQAEWIWGTVTQV
                     PTILSLLTPEYQGRMVQDNYHEAVDNAPQWEASFCWPEGFMRWWAQASRGGDFQLTIT
                     PWSAEFLSGIAANFLRQVWIGRQPVQQVPQWYGETVGFWDGTTLVTWTSHVQAWNLSH
                     SMFENSGKMETVEIWKPVKDASGKYVGLDQETIFYDPEAFVQPLRATYRYRRTALLDS
                     PTMRYTYIECLSNIKDRNGRATQLTSDDPDYVDYYGRPWAEDWEKYFEKGWKDTPNEN
                     PVPDDILNMLNKDKK"
     gene            complement(13825..14928)
                     /locus_tag="VN788_02895"
     CDS             complement(13825..14928)
                     /locus_tag="VN788_02895"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXU19511.1"
                     /translation="MRCKTAFVNFMWIWAPALLLCQALTVSPVAAQNSTCMPTAEQAA
                     QLFSAGARGPSRPNPEPAAEAAPRNEGITAIPGVVTSGGKWIKVWQEGGNSADGIIAD
                     KDGSVLVAQEDYDAVLKINSNGQTSVDVAEAKGVGSLSMDRQGRLYGAHRTERPGSTK
                     PDKASIENAITMLAPERMIIADKWTDGTTLTVRPNDLAADSHGGAYFTSGCLYYASSK
                     GASVMADNIRTNGIAFSPDDKILYVTNGGQLVAFDVEGPGMLTNRRDFAMLPAGNSGD
                     GMAVDSKGRLYVSSREGVQVFDKTGKHLGVIPTPRGIISVAFAGPNKKTLYVVGSGAD
                     DEKGQPIRQGPQHTAATVYKLPVIAQGLKDRAK"
     gene            15117..15992
                     /locus_tag="VN788_02900"
     CDS             15117..15992
                     /locus_tag="VN788_02900"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU19512.1"
                     /translation="MHYVSRIDERAAETPQGYAKHSNQFRRATYVDRAIGSVHMGVGI
                     CFLEPQGSIQTHVHSFEESFYILEGSVAVQIGEGTHSLGPGHFGLVSTGVRHSWRNTS
                     NRPVRWLEMQAPQPRPVGHSQDTFFVGNDVVVPAASPATPNSSDSPLGHFDESKLPRP
                     GGPSQMEGFNPTTGVAIKMFVDRSLGAIHQSLFLIQYSPGAKIDPHDHTFEESYLIVA
                     GRVRATADGKTYDLGPGDVIWTGVGCIHSFENAGTEPVRWIETQAPLPPAKEVFRFER
                     DWSHLVQHDKEDAPR"
     gene            16173..17177
                     /locus_tag="VN788_02905"
     CDS             16173..17177
                     /locus_tag="VN788_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXU19513.1"
                     /translation="MTRKTKIVEKSSAAGGETEKWLHMYRNMVAIRLFEEQVNDLYTR
                     TLMPGLAHLYIGEEAVAVGVCSALRPDDYITSTHRGHGHCLAKGASPDRMFAELLGKE
                     PGYCRGKGGSMHIADPETGNLGANAIVAGSTGIATGAAFSAKRLGNGRVAVCFFGEGA
                     LGQGVLYESMNLAQLWKLPVIYVCENNTYNEYTHYSETTAGEVLGRPKAFGIHAESVD
                     GQDVRAVYATAKNVVERARGGDGPAFLLCNTYRYRGHHVGDVNREYYRSKKEEQKWMS
                     ERDPIKLLRQGLIAENDADAAVLDSIEAEVKSNIDEAVKFAIAAPYPDPNEVQQNVYA
                     "
     gene            17164..18192
                     /locus_tag="VN788_02910"
     CDS             17164..18192
                     /locus_tag="VN788_02910"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXU19514.1"
                     /translation="MSTREMKTALQEPSVAPRELTMAEAIKEALAEEMRRDPRVFIMG
                     EDVAEAGTVFKVLSGLSAEFGTERVIDTPISEAGFTGLGIGAAMTGLRPVVDIMFGDF
                     IALVMDQMANQAAKVHYMSGGKWKVPMVMRTTLGATRRSAAQHSQSLHAWFSHVPGLK
                     VVLPSTPYDAKGLLKSAIRDDNPVVFFEDKMMYRLKGPVPAEDYTIPLGVADIKRSGD
                     DITIVATSSMVQVALGAAKMLEESDISAEVLDPRTTWPLDEDTLIESAKKTSRAIVVD
                     EGYARYGVTAEIASVIAEGAFHYLDRPVKRIGAMHVPIPFSPPLEDVTVPTEKMVFDA
                     ARAMCSGS"
     gene            18227..>18808
                     /locus_tag="VN788_02915"
     CDS             18227..>18808
                     /locus_tag="VN788_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HXU19515.1"
                     /translation="MALPTHGTMAVDWEQRIDFDRLRRERLARIKSLLEKSELGALLC
                     FDMNNVRYITATHIGTWAQDKNSRFTLLSRGAEPIVWDFGSAAKHHQLHCPWLGERSR
                     AGIALLRGAMSPEMGRAEDVAKKIKVELEKLGLEKEPLGVDIIEPPILFALQKQGIRI
                     VDGQQMLSDARVIKTQDEIALLNHSAMMVDAAYD"
BASE COUNT         4160 a         5245 c         5148 g         4255 t
ORIGIN      
        1 agactggagg cctacacatt tttcactttc gcggaggata cggcgagaag cccctgggaa
       61 ccatatcacg tgagcctcgg ttacaaaccc gaggacagca cggtgacggt ggcaacatct
      121 gggactgctc cgctggtttt cggcggcgga gccgtgatgc cgtggactgg acagggcgtg
      181 atcgattcga tcgtcgctca aatcaagagc cgaggagtgg tttggcctca ctctcaaact
      241 tatatcctcg ctcttcatcc tgagtgcgcg gtcgatctcg caacgcgtgg ctacactcgg
      301 aaaagtttgc aggaatacct tttcgaacga acacgtgtat cgtatgaaaa gctggcggga
      361 ccggtatttt ttggtcacga caacgaaatt gactttatcc gcacatccat agccgacgga
      421 cgaattcgcc ccgaccgtgc cccacttttc ctggaggcgc tgaagcctgg gggtttcgta
      481 cctgtggttc aaagccctgc cgattttcac atcgtagttg ccggtggctc acccggatac
      541 gatctgctct tcagttaccc gggaacgaac tgggcgaatc aaaccaaacg aatttcggca
      601 gcgatgttga cgaccgcggg acgcggctaa gccactgtca tagattgcca ggccggcggc
      661 gctcctttta agccgaaggt cgtgggttcg atccccaccg gcctaccaaa gtgcgcctat
      721 gcgggaatct gcgggaccac caggtgcaac aacttgagcg aggcaccctc cgcgggctgg
      781 atcgtcgcgc tctctgaggg gccaagatac acgccggcgc ccttcaagac ttcgtaatcc
      841 ttatcgtcga ggcgaatgcg tccactgcct tccatcaggt aaaggagttg atacttgtcc
      901 gaagcttttg cttcgaacct ttctccgggc ttgagccaac gcaaggccgc caccacattt
      961 tttgcgccgc aaagttcccg gttgagcact tcggtgactt cgccctcggc agtttttttg
     1021 cgcggaagct tattcgtatc gatgaaagtc agcatgaatt ctcctttggt ccgaccgtgg
     1081 acttaggtct gaacccagac gccgccctcg acgcggttcc agcgaaggtc gtcgggggtt
     1141 ccgtctagaa tcgtggtgac gaagcgattc ggcgcctgaa attcaaaata gaacaacggt
     1201 gttttggagg ctcgcaggcg atgcttcgcc tcggcgggaa taaaaaccaa gtcgcctggc
     1261 cccacgtcac gcgtttcttg ctccgattcg accgtgcctt gtccttccat gatgaaataa
     1321 aaatgctcgc aattcttatg aaggtggtag ggcgaggaag cacccttcac atagtgcatg
     1381 actcccgcga gcatgtcgtc gattttagtg agttccttgt tgacgaagaa cgtgcgctcg
     1441 cggccgggca ccgcggagat cagtctcggc agatcgtttt catgaaatat gtatgccatc
     1501 gaattctctc ctaagcgaat gcggtttcag tgtggccggt acgcgacgcg tgcaacctcg
     1561 gcgtagggta cagcggcaac cgttgctcgg atttcagctt gcctgtggcg ctcgaccgtg
     1621 ggtttcggcg atccgcgatt ttcttccccc catacgaccg tgacctctgt accaatctca
     1681 ctgtgctggt tatccacaac ggccagggag atcatcacac gttcattata ggtgtagccg
     1741 gtgtaagtgg atacgccgat ggttttgccg cctttcacta ccttatcgaa tggaagcgat
     1801 gaatagttag ccagcggagt atcaatgtac ttgaaaatat caccaccctc gtataaagaa
     1861 ccggtaacac gtttgatatc gtccttgttc cacaccagcg ttaccttctt cctcttctga
     1921 tcatttacca ttttttcgag ggcttgtttg ccgacaaaat catggtcgaa tttgacgaaa
     1981 ggcccgtagc cgaggtcgta gggagtcaga taatagtccc tgatatcgct ggcgtaaaag
     2041 ctacctccga gtgaagccgt ggcttcgtag ctcgttggct tcagccactg tcgatatgcc
     2101 ttcatctctt caccggaata aatcgccgga agcggcgagg gaatccatcc cgactcgagg
     2161 caggtggtcg ggtaagctcg agagcctact tgtcgaattc cgaattcttg accgccttca
     2221 acgatggcgt tgcggacttc atcgccgtac tcccaggggc catacaattc ccatccgggt
     2281 tgcccgacca ttccgtgacg caaggcacta acacgccggc ccgcgatggt gagtacgtct
     2341 atgttaaaga accgaatatc cggcgtaggc tttcccgtga ccttctccat aacccgcaga
     2401 gcattcgggc cttgaatctg gtatcggaaa gcttttcgtt ggccttgatt gacagctgac
     2461 ctctcatctc tttccgccga agcccgatac ttgccagtct ccacattata ttgcacccaa
     2521 ttgtgagcgg gcgggcgtcc gacgagattg aatctatttt cattgagata aaagagaatc
     2581 gcgtcaccaa tgacgtagcc atcatggtta cacgcgacaa attgcttggc ttgattgact
     2641 ttgaagtttt taaagctatt gatagcgaga tcggagaata atttgagggc gtcaggaccc
     2701 tccacataaa gatcggtcat atggtgagat tggtcgaaaa gagcgcagct ttccttccat
     2761 gcccgctgtt catctctcca attcgtaaat tccggacgca ctaccggaaa ggcatatggt
     2821 cctatctgag aatttcgcaa cagctttact ggactcccga cggtttgtaa cacatcctcc
     2881 aagcttcgat aagtcatccg cagtagctcc ttctgctttc gaaagcatag gttcgcccaa
     2941 agaacctaga acgctatacc aggcgtagta ttctgtaaaa tacattcttc gtacctaaac
     3001 cagacttttg ggatatagga cgcgaggagt atatcgaggt ctatctttgg agcttcgaca
     3061 tctacgctat ttccttgccg tggccgatgc cttgaatttc tcccgggctg ccgaacgact
     3121 gcatttgagc cagccgccat taagtcaaca ggtccgccgc ctggaagcgg agctcggtgc
     3181 gaaacttttc gataggagca agcgccaaat taaactgaca gctgccggtg agatctttgc
     3241 caccaaggca cgcgcgatcc tggcgcaatg tgatgaggct gcaaagctgg ctgcgcgcgc
     3301 ggagagaggc gaattcggtc acttgcggct ggggctctgg cagctcccgg tgcccccggt
     3361 tcaaaccaga ttgatcatcg agtgcctgcg agcattcgtg aagcgcagcc cggatgttca
     3421 tttgaccttg cagtcgttgt taccggcaga gcagatcaag gctgttaaag atggtggaat
     3481 cgatgtgggc atcgtcagat tacctgttgc ccagaagagc agaggtattg agattgaaac
     3541 catcctgcga gagccgatgg tgctcgcaat gtctgagaat aactcactcg cgcggttcaa
     3601 aacggtacca cttcgagagc tctcgaacca acgctacatc atgttcgggc gcgatgcgaa
     3661 tgcggatttc caccatttca ttgcaagagc gtgccgcaag gaaggcttta gtctcaatat
     3721 cgcgtacttt caagacgatc tttattcggt ctgggcgctg atcggtgctg gtctaggtgt
     3781 ggctttagct ccggcttcgt tacaggagct acggctaccc ggaattgtgt ttcgcaaatt
     3841 cgagcccgca gctccagtaa tggaaatggc gctcgcacac atgcacactg acaaagtgtc
     3901 tgtggtgtct caattcctcg aagttgccag aaaaatagcc aaggcgtact caggtggcca
     3961 ttgagaaccc caagatcgcg tgatccgttc taatctggtg tcgtggctat ccgttcacac
     4021 gctcatgctc cggctccagc atgacggatg gcttcgagcg cctgccgctc ctcgccgttc
     4081 ggatcatcga cgacttcgca ctcgttgttg agaatcatgg tggcgcgttg tttggtattg
     4141 aaggcgggcc agtttgggat gcccttgtgg cttgggttcc ctgtacgcgc aaaagcaacc
     4201 caggcgctgc tgattttgtc ttcaagggca tagcgatcct ggccggagcc agtcatggcc
     4261 gtcgccaaat cgacattgtc aaaaacaaac ggaatttcga gcgtgtggaa ggttctcaac
     4321 tttccttctc gcaccggtga tttccaggtg aagtaataca tgtagacagg cgccattcct
     4381 tggtccgctt tgcgctcagc ttcagtaagc acgcccgccc ggaacgtggc gtccgatgca
     4441 agtaccagat agagatcggt attgctgatg ttcgggcggc cctttcgata aacttcgatc
     4501 agccggtcca cagagtcatc ggcagcgccg cgtagtgctt gcttgacgtg catgtgcaga
     4561 gcggcgtcgt ccatcggatc caccggcgtg ccacgcatga aggtgacctc ggtttcaacg
     4621 gacccgatca gcagcggaac ggttgctgag atttccggtg catcgggatc gaacgggttc
     4681 ctcggaagcg aatgaccatc caccacgggg gccaagcgga gaccaccccc gccagccgca
     4741 cctccggtgc ccattgccgc gaggagttgc tccgcaggca tcttctggac atcgtcaatc
     4801 tgattggact tcactccgag tctccctaga tatgcctccg ctgactttgt tgcttcatct
     4861 tttggcacac ccctgattgc cgaggcactt tccacaatgg cgcggtgaaa caggcccctg
     4921 gccgatggca ttgccatcag ggtgctgact tttccgccgc cgccggactg gccgaagatc
     4981 gtgacgttgc tcggatctcc gccaaatgcc gaaatgttgt cccgtaccca ttccagcgca
     5041 gccacgatgt ccagcatccc aacattgctc gagtttgcat acttctcgcc acccagctca
     5101 gcgagataca gatagccaaa cacattgagc cgatggttta cggtgacgac cacgacgtcg
     5161 tgccggcacg ccaagttcgc tccgtcataa attatgaaag caccggatcc ttgggaatac
     5221 cctccgccgt gcaaccacac catcacgggc ctattgcctt tccctaggcc gggactccat
     5281 acgttcaaac gcaggcaatc ctcgctcgcc ggttctctcc gatccacagc ggcgacctcg
     5341 ggaatcaaac cggacgggcc ttgaggcgat ctggggccga attccaaagc gtcgcgaacg
     5401 cccgtccaag agtcgggttt ggatggcggc ataaaacgcc gtgcatcggc ggtcgacgca
     5461 ccgtagggaa tgccaaaaaa tgcgcttact ttatcgcgcg ttatgcctcg gactcgtcct
     5521 gcagtcgtgc gaacgacggc gctgggcgtt gaacacccaa gatcctgcgc caacgcttga
     5581 tgaaatgccc cgcgcgcaaa aagactggct gtcgccgccg tgccataact gagaaaagat
     5641 cttcggtcca tcgccgtccc cccttcaaat ccgaaatgtc ttgggtcctc atccttcatt
     5701 tatgcgtccg cgaatgagac tgccattgat acgccctgtg atctaaacca ctctggcgac
     5761 aaagtccaat acattatgat tgctggccat gccgcagcgg tcgagtggca taagctattt
     5821 atcaaaagaa ttgaatgtga cgcgtgtcgg gcccacggaa tgccgcataa gactcagccg
     5881 gttcgataac tttattatta aaataatttc atctaattga catcactacc tgcagggcgt
     5941 acatatgcgc caagccgaaa agggcagcag gacgggtgtt cgacaatttt cagactgatc
     6001 gaccatcgtt cagcacgtac ttgtcaggct tggtagcgat atatgggtgg gctatccacg
     6061 aagttatttc gaattattag cgctcgatgc gcattgcgtt agcgtcgcgt ttttcggctt
     6121 ggcgccgcca aagttttgcg aagacgactg gggggggaat tatggacacg tattcaatgt
     6181 tttcctgttt gcggtgcctt agtactaaga aggcatttca ggtgttagct ctcgggttgg
     6241 gagtgttgct actctgcatc cctgcttttt cacaactcaa tctgggaagc attagcggga
     6301 ccgtaacgga taccagcggc gcggtcatta ccggcgccac cgtgaccgtc accgacgtgg
     6361 aaaggggtgt ctcgcgaacc ctgaccacgg atacggccgg ccagtattcg gctccaagcc
     6421 ttactcctgg cagctactcc gtgcgcgtgg agttcgcagg gttccagaca ctgaaccgaa
     6481 cgggcatcgc ggtcggagtt ggtcaggcgg tccgcgtgga tctagcgatg cagccgggcg
     6541 cccaaacgca gacggtgacg gtaacggaag ccgtgccgct cataaacact gccaacgagg
     6601 tgttatccgc cacggtggag gcgcctcagc tcgccgagtt gccaatcaat ggccaactgt
     6661 acacgaaagt gctggaattc cagccaggca tccatgggaa cccgggcgga aattcgccga
     6721 actactcaac caacggtgct agcacccagg gcaattactt tatgctcgac ggtgtggaga
     6781 atagcaatat attcgtcaac tcgggacctc tcatcggcgc ggcaacgagt accgacgaac
     6841 tgaccatcct gccccaggat gccgttgagg aagtcaacgt gatgaccaat ccaccagcgg
     6901 aatttggatg gtttcagggt gccgtggtca atgtcggtct gaaatcgggt acaaacgccc
     6961 tacacggtac cgcctacgga tacggccgca acaacaactt ggatgcgtac gacccatatc
     7021 tgaatggcgc gccaaagcag gacgataatt tcaagcagtt tggcgggtct ctgggcgggc
     7081 cgatcaagaa ggataggttg ttcttcttcg gcgcctatga cggaatgcgc tacaaggttg
     7141 gaactgctgg caccgcaaca gtgcccaccg ttaatggggg ggcggttgat gctccgtcca
     7201 acagcgtacc gcttgccatc gatgacatga ttaacaacca cggcgttttg ccgaatcaac
     7261 tgagtctgaa cttgtcggac tgcaccgtgg cgggctcagc gcccactgcg gtagccacct
     7321 gcggaacgaa gggtgtgtgg agaaacaatt cactccagac gataggtatt ggcgtgccgg
     7381 gggattcatt cggtcattcg aacaatgtaa tcggacgagt cgattttcat cccaacgatc
     7441 gcaactcaat caatggcgaa tatttctttg gacaggcagt tacctccaca cccaacgttg
     7501 gcgaaccatc cttctggaat aacttgaacc tcagtcgcac gcaaatgatg cgcgcggtgt
     7561 ggacctatac gccaagttcc aactgggtga acgacgttcg gttcggctat aaccggtaca
     7621 acctcgcaga cggcaacgcg gaatgcaccg gcaacggcaa cttctcgtcg ggaaatgtgg
     7681 gccagccaaa ctatgcggcg cttggcttcg tttccggagc gaatccacct gccccgttct
     7741 gcggattccc ggccgtgaat tttaaccagt ctggctttcc cagctttggt gctctgtcgt
     7801 tctttatcag cgaccaggga gtgttccagt atacctacac ggcgtacgac aatgcttcct
     7861 atacgcacgg cagccacaat ttccatttcg ggtttgagtt ccatcacagc aggttccatg
     7921 ggataggcgc gccaggcttt cttgacggcg tcctcaattt tgatgggggg gttgcatttg
     7981 gaccatgtgg cgcagttggc gaaccggcgt gtccggccgg agctgcagcg ggagaaaacc
     8041 tggcttccac ggatctgcag gatttccttg ctggatcact cggcgcaaac aaccaaatcc
     8101 tggtcaatcc tcagcaagag gacaccggtg ttggctttaa ccgcgaagct ctctattttt
     8161 cagatgactt ccgggcgagt tcgagagtaa ccgtgaacct gggtctccgc tatgaattgg
     8221 agccggcgat taaggtagac aataacactg caggcaactt cagtgcgtct tcgccgacag
     8281 gcatggtaca gcaaaatgga agcgccgtgt atcaatcctt ccacaaagca tttgcccctc
     8341 gggcgggctt cgcctgggat atcaccggaa agggaaccac ggtcctgcgt gcaggaatgg
     8401 gagtcagctg ggatacacct caggtcgacg acttagttgc ccagagtttt ggcgcgggta
     8461 tcaacgctat tcctaccgga tttagcctct tcaatgatgc tggcttggtg tttccagctt
     8521 ctacaaatcc gcaggccgtc aagacggccg tggtctccat cccgggaccg gggttgacct
     8581 gggcgcttaa cacaccagtg ttcggcgcgg gcctcaactc cgacttggcg tgcggcaccg
     8641 gtaaccccgc gatcgttctg cccgacggca gcaaccccag cccctgcacg cttaaggcta
     8701 ggggcactgt cgttaggcta ccgggcggtg gactgtcaat gaacacccag aatgcccggt
     8761 ctccgatgta cacgtggacc ctgggcattc agcacgcctt tggggctaga acttcactga
     8821 ccgtgaacta tgtgggcact cacacgtacg atctagcaag tgagatcaac atcaaccagg
     8881 cggcgccggg agccacgggt gctggactgc tcccgccggg tgtcggccta ggaactgtcg
     8941 atggacccct gcagcaacgg cagccgtatt ttggccagtt tccatggttc aacggcatct
     9001 tcgtatatgg gccggctggg ttttccaact accacgcact acaggtgagc tttgttcagc
     9061 ggagtttcca tgggttgacg ctgaacgcgg cctacacatt ctccagggat ttggctactc
     9121 caaagggtgg caataacccc tacatagata acagccagtg cgtgtcatgc gactatgggc
     9181 ttcagacacc cacgcaggac ctgggtgtta ctctggttta cgccgttcca gggtttaagg
     9241 cgccagcaca gatgctggag ggatgggaga tctccagtgc gatagacgtg caaagcgggg
     9301 acccttttac cggtctcgat ggcagtgacg acttcgctgg agtcggcaac ggaagaaacc
     9361 ttttcggcgg agctagcgaa ccatggtccc tttttggcaa ggggaccaat ttcaagggca
     9421 ttggaaagct tgcccctgtt ccgtgcttcg ggtcgagctt tgttttcgcg gttttggggg
     9481 gatgcaaccc ggtcctcccg gccgcttgca taagcgcagc caatgcggaa ccggtgaatg
     9541 ctgcaatgaa tgcgcagatt ccaggttcgt cgagcggatt ggcttccctc atgtcagcgg
     9601 ggtgctatat gtcccccaac ggcaagtcgg tgatcattcc gccggctcag ggaactttcg
     9661 gcaacatgag gcctggtgca ctcatcggcc caccattcca tgagtgggat ttgtcgctgc
     9721 gcaaggcgtg gaagatccat gagcggctca atctgcaggc cagcctctcg gcctacaacg
     9781 tgttgaacaa ccatgcatac gccggcggat ttacgggtgg tatcgtaaac ctgccattcc
     9841 tattcggcca atcgagtggg caaccaaacg acggcaatcc gatcaacggg accggcggcg
     9901 ctcggatagt atcggtgggt ctgaaggcga ctttctgagc ggctttaagc tctaaacatc
     9961 cacggttgtc gtcgcttgag gttcgtaatt cgctgtcgtg cagccggcgt ggtgttcggt
    10021 ctgatcctcg ccggatgcag tggagaaacg tccgctccaa cgagcggtcc tcgtccgctg
    10081 cgctggaaca atccgagcct acgaaggttg tcgatcccgc cggaacgatc acagacacag
    10141 tcactctgga aggaccccgc ccgcagggca tgagattacg ctgggcgcgt cgccagcgtg
    10201 cgcatagctg tattcatcca tgggatgtgc acctgcgttc tcgccttttc gcatccatat
    10261 ttcgacgtca cgtcgaagaa gggcgcattt gaattgaaga atccccccgg gcacgtacac
    10321 gattgagcca tggcaaaaaa agtacggcac gcaagaccaa agtgggacgc cgaccgcgaa
    10381 aggacgaaag tctatttcgt ttacgttcaa ggcctgtcac agctgaggga acctacaacg
    10441 cgggcctctg cacggctccg attcacctct gaggttgatt agctggtggc ttcgctgggg
    10501 ccggggctgc gccaccatta ccggtcggcg cctgctggag aaaattatca aaattgacgc
    10561 cgggtggcgg cggcagcact ttgcccgggt gggttgtgaa accaatcagg cggcccatga
    10621 atatgatgct gatccataag gtcagtgaca gaatcgccgc cactttgcct gcacgaggcg
    10681 gtgatggatc cctatcccaa cgatggaccg ttcggcgccc ggttatctca aacacggcca
    10741 tgttcacgcc ggcgagtacg agcaacagca tctttgtcct gaaaaagggg ttgtgataat
    10801 agacgcgggc attcgtagtg aacatcatca ctccggtcgc cacggccaaa acaaaagcgg
    10861 cccacgtcca cttcaggatg tccgaggaga tccgctgaaa cgacctctga gttgaagcaa
    10921 gcccgagcag ccggagatct attatcgcaa ttgtcccgac aaccaaagtg aagctcatga
    10981 cgtgaatcga ttcgattatc ggaaacatcc agatcgaatc gcgaatggtt gtggcgaagc
    11041 gagtggcttc gattaccttg aggaatgcat caatctgcat tgaggctttg ttccaaaatc
    11101 attaatattc ctgcgttgcc ggcgaccacg aaccccagat gtggtcgtaa gcgatgaggc
    11161 gtcccagcac aataatgaac agaaaaatca ggaacgtcag tattgctagt gacttgatgg
    11221 atctgtgggt gacgagcgtt tcccaccgtt gctcgtgtct cggaaccctg accagaaaaa
    11281 taccggaaag cactgtagct atggctacga aaaccatttt cgtccaaaac gagaaattca
    11341 ccagttcacg agcgggctcc gcgattatca ttacgatccc ggttaccaac tgcaggcaca
    11401 atccgcctat caaccacgga ccgaaccgat ctgtggtctg gcgcagcgtt tgatcggtcc
    11461 cagcccatcc cagaatgcgg agaacgatca tgaatacgga cccgattatg actgcaatgc
    11521 caatgatgtg gatggattgg acgcccggga taagccaacc ctcttgaact cggaaggtgt
    11581 gactcaggga cgttttggtt agccattcag cgaatcgact cacgccaaac caccttcctt
    11641 accatcgacg aagatacaaa tagaactaga accttccgct aatagtcgtc atgccaggca
    11701 ccgtgtcaca ggtcttaccc gcagctgggg gcgtcctcca agggcatttg taaatcggac
    11761 caacccggga cccgaaatta ccgccggttc ggagaggatt catgtgcaca gtgaatatcg
    11821 tgcccgcctt gaacgagtcc gcggtaatcc cttgctgcgc taccgctgcg gcacccgcca
    11881 tctcgacgcc ccaatcgatc agttgtccgt ttttgtcctt ctcaagtttg ccgtccggcc
    11941 caatcaggta aaagtgcaac tcggcatgat tcgcctgggc aatgaattgg tgggccacgc
    12001 cggttaacac taataccttg gtctgatcgt acatggcgaa cgagtgatgt gcgaccgccg
    12061 gcttgacgta gatacccaac gatagaaatg ctacgcacgc agcgatcacg cagcttgtga
    12121 ttagtgtttt tttcatcttt gcgctctccg ttttgtgtgg cagcctcaag ctatcgatgg
    12181 tgcctcgcgc ggccgctcaa gtttttactt cttatcttta ttcagcatgt tgagtatatc
    12241 gtcaggcacc ggattttcgt tcggcgtgtc cttccagccc ttttcaaaat acttttccca
    12301 gtcctcagcc cacggccggc cgtagtagtc gacatagtca ggatcgtcgg aggtaagctg
    12361 agtcgccctg ccgttcctat ccttgatgtt gctcaggcac tcgatgtatg tgtatcgcat
    12421 ggtcggagag tccaacaacg ctgtccgacg ataacggtag gtggcacgta gtggttggac
    12481 gaacgcttca ggatcataaa atatggtctc ctgatcgagt cccacgtact ttccactggc
    12541 atcctttaca ggtttccaga tctctaccgt ctccatcttt ccgctatttt caaacatgga
    12601 gtgcgatagg ttccaggcct gaacatgaga agtccaggta accagggtgg ttccgtccca
    12661 gaacccaaca gtctctccgt accactgtgg aacctgctgt acaggctgcc gtccgatcca
    12721 aacctggcgc aggaaattag cggcgatgcc agatagaaac tcggcgctcc aaggagtgat
    12781 cgtgagctgg aagtccccgc ctcgcgaagc ctgagcccac cagcgcatga atccctctgg
    12841 ccaacagaag gaggcttccc attgcggcgc attgtccacg gcttcgtgat agttatcctg
    12901 aaccatacgc ccctgatact cgggagtgag taaagacaga attgtaggaa cttgagtgac
    12961 ggtcccccaa atccattcgg cttgagtatc cgtcgtcatg tcccgcgtat accatccgtc
    13021 ccaatccgga gtcgtcgcct tggtgtagac agtcgggcca cctttcgcct tcgctgccgc
    13081 cagcaaggcg tcatattgct ccttagcggt cttataaggg taatggctca caagctgatc
    13141 tcgcgggata tcccacttgc agtcgcccca agccgcgttt tctggcgctt tacccgggcc
    13201 aaaaaaacgg cgcgatgtcc acatatccgt tagctggcga ggcgtattgc aacgaaaata
    13261 gcgcggatct gaccagtact ttttatccaa atagaagttc ttcgacttaa acaaatcgat
    13321 tggcaatgcc ttcattcctg caggtggccc atcgaaagtc ggcgtacata tgctgagtgg
    13381 cccgatgaca tttgtatatg gtccgtgcgg tccggaaagc gcgttaccgc ggcctcgaaa
    13441 ttggccccgt gtcccctgag ccttcgcgca ctgctgctcc gcctgtgggt cttgcttggc
    13501 ctgcggacct tgctggtcct gtgctgacga cacaggacac atccaggtga gcataatcag
    13561 ggccatgaag ccaaacacaa ctacaagccg tctactgttc atctatcgct ccccctcgca
    13621 atgagacgtg cgaggttata tggcggtttc cgaaacttcg agagaaatta gaccggtctt
    13681 tcctgtcctg gccttcgtta cgcactaaaa tcaaagtgac accgagtggg gataccctcc
    13741 cccaaagtta atgtcaacag aaaattaatt aaggaaaatt aacaatgcca gcgacgcaag
    13801 atgagacaag tggtgaacta agacctattt tgcgcggtcc tttaggcctt gcgcgattac
    13861 cggcagcttg taaacagtcg cggcagtatg ctgcgggcct tgacgaatcg gctgaccctt
    13921 ctcgtcgtcc gcgccgcttc ccaccacgta aagtgttttc ttgttgggac ccgcaaaagc
    13981 gacgctgatg atcccgcgcg gtgtcggtat tacgccgaga tgctttccgg ttttgtcaaa
    14041 aacctgaacg ccctcgcgcg aactcacgta cagtcgtccc ttcgaatcga ccgccatacc
    14101 atccccggag ttgcctgccg ggagcatcgc gaaatcgcgg cggttggtga gcatgccggg
    14161 tccttccaca tcgaaagcca caagctgacc gccattggtg acgtagagaa tcttatcgtc
    14221 cgggctgaac gcaatcccgt tggtgcggat gttatccgcc atcacgctgg cgccctttga
    14281 actggcgtaa tagaggcagc cgctcgtgaa gtacgcgccg ccatggctgt cagcggcgag
    14341 atcgtttggc cgcaccgtca gcgtagtacc atccgtccat ttgtcggcta tgatcatgcg
    14401 ttctggcgcc agcatggtga tcgcgttctc gattgacgct ttgtccggct tcgtcgatcc
    14461 gggtctttcc gttcggtgtg cgccgtagag ccggccctgc cgatccatcg acagtgatcc
    14521 cactcctttg gcttccgcca cgtcgacgga agtctggccg tttgagttga ttttgaggac
    14581 tgcgtcgtag tcttcctgcg ccacgagcac gcttccatcc ttgtctgcaa taataccgtc
    14641 tgcgctgttg ccgccttcct gccagacttt tatccatttc ccacccgagg taacgacccc
    14701 tggaattgcc gtgatgcctt cgttgcgtgg cgcggcttcg gccgctggct ccgggttcgg
    14761 tcttgaaggt cctcgcgcac cagcgctaaa gagctgcgct gcctgctccg ctgtcggcat
    14821 gcatgtcgaa ttctgcgcag cgacagggct caccgttagc gcctggcaca acaagagcgc
    14881 cggtgcccaa atccacataa aattcacgaa tgccgttttg catctcattt tgcctccgag
    14941 aagttacgag aacggagatg ttacaccgag atgccgcgcg gagatactac ccaacttagt
    15001 ttcatatcga atcgctgtga tataaactct ctcgtccacc acggtacaaa agctcgggca
    15061 gaccgggccg gttcgtgtcc ccaaggcttg agccaatatc gagacggagg gtcaacatgc
    15121 actacgtatc cagaatcgat gagcgcgctg ctgagacacc tcaaggctat gccaagcata
    15181 gtaatcaatt ccgacgtgcc acatatgtgg atcgtgcaat cggttcggta cacatgggtg
    15241 tcggcatctg ttttctagaa cctcaaggat cgattcaaac gcatgtccac tcttttgaag
    15301 aaagcttcta cattttagag ggatctgttg ccgtccaaat tggagaggga actcattctc
    15361 tcggccccgg ccacttcggg ctggtgagca ctggcgtgcg gcatagctgg cgcaacacga
    15421 gcaatcgccc tgtccggtgg ctggaaatgc aagcgccaca gccgcgtccg gtcggccaca
    15481 gccaggatac gtttttcgtc ggcaatgatg ttgtcgtgcc ggcggcatcg ccagccaccc
    15541 ccaattcgtc ggacagcccg cttggccact tcgacgaatc aaaattgccg cgccccggcg
    15601 gcccatctca aatggaaggt ttcaacccaa ctaccggcgt ggcaataaaa atgtttgtgg
    15661 accgctcact cggagcaatt catcagtcgc tatttttgat ccaatattca cctggcgcaa
    15721 aaatagatcc gcacgatcat acgtttgagg agtcctatct cattgtagcc ggccgcgtcc
    15781 gcgcgacagc ggacggcaag acgtatgact tggggccggg agatgttatc tggacgggtg
    15841 tcggctgcat tcacagcttt gagaatgccg gcacagagcc ggtacgctgg atcgaaaccc
    15901 aggcgccgct gccgccggcg aaggaagttt ttcggttcga acgcgactgg tcgcatttgg
    15961 tccagcatga caaagaagat gctcctcggt agttggccga gttgcgttcg attcgccagt
    16021 cagcagttga aaaggtggct gaccggactt gtgagcttaa atctcgccta gtacactagg
    16081 aacggatctc gttctcacgg cgagagcgga aagcgattga actggcatct gctgttatct
    16141 ctgtttctac gggagaaggt tgagaaagtg gcatgacaag aaaaaccaaa atcgttgaga
    16201 agagttccgc cgccggcggc gagaccgaaa aatggctcca tatgtaccga aatatggtgg
    16261 cgatccgtct attcgaagaa caggtaaatg atctctatac tcgcacgttg atgcctggac
    16321 tcgctcacct ttatataggc gaggaagctg ttgccgtggg cgtttgttcc gcactgcgac
    16381 cggatgacta catcacaagc acgcaccgcg gccatggtca ctgtttggca aaaggcgctt
    16441 cacctgatcg aatgtttgcc gaattgttgg gaaaagagcc gggttactgc cgcggaaaag
    16501 gcggatctat gcacattgcc gaccccgaaa ccggcaatct tggcgcgaat gcgatcgtcg
    16561 cgggcagcac cggtatcgcc accggtgcgg cgttctctgc caagcgcctt gggaacgggc
    16621 gcgtcgccgt ctgttttttt ggtgagggcg cgcttggcca aggcgtgctc tacgaatcca
    16681 tgaatcttgc gcaactctgg aagctgcctg tgatctacgt ttgcgaaaat aatacttata
    16741 acgaatacac gcattattcc gagacaacgg ccggcgaggt tctaggccgg cccaaggctt
    16801 ttgggattca tgccgaaagt gtagacgggc aggatgtgcg agccgtgtat gccactgcga
    16861 aaaatgttgt cgagcgcgcg cgtggaggcg acggcccggc cttccttctc tgcaatacgt
    16921 accgatatcg cggccatcac gtcggcgatg tgaatcggga atactacagg agcaagaaag
    16981 aagagcagaa gtggatgtcc gaacgcgatc ccatcaagct gctgaggcaa gggctgatcg
    17041 cagaaaatga tgctgatgcc gctgttctgg acagcataga agctgaagtc aaatcgaaca
    17101 ttgatgaagc ggttaagttc gccatagcgg cgccgtaccc tgatccgaac gaggtccagc
    17161 aaaatgtcta cgcgtgagat gaaaaccgct ctgcaggaac catccgtcgc tccgcgtgaa
    17221 ctgactatgg ccgaggcgat caaagaagcg cttgctgaag agatgcggcg cgatccgcgc
    17281 gtattcatca tgggcgagga cgtagccgaa gccggcaccg tcttcaaagt tctctccggg
    17341 ctttcggcgg aattcggcac ggaacgcgtg atcgatacgc cgatttccga agcgggtttc
    17401 acaggcctgg ggattggtgc cgcgatgact gggcttcggc ctgtggtcga tataatgttc
    17461 ggcgatttca tcgcactcgt catggaccag atggccaacc aagctgcgaa ggttcactac
    17521 atgtcgggcg gcaaatggaa ggttcccatg gtgatgcgga caacgctggg ggcgacgcgg
    17581 cgctccgctg cacagcattc gcaatctctc catgcctggt tcagccacgt tcccgggttg
    17641 aaagtggttt taccctccac tccctacgac gccaagggtt tgctcaaatc cgcaattcgc
    17701 gacgacaatc ccgtggtttt tttcgaagat aagatgatgt acaggttaaa aggaccagtt
    17761 ccagccgagg attacacgat ccccttgggc gtcgccgaca taaaaagatc cggagatgac
    17821 atcaccatcg tggcaaccag cagcatggtg caggtagccc ttggcgcggc gaaaatgctc
    17881 gaggaatccg acatcagtgc cgaggtgctt gacccgcgca caacgtggcc gttggacgag
    17941 gacaccctga ttgaatccgc caagaaaact tcgcgcgcga tcgttgttga cgaaggttac
    18001 gctcggtacg gtgtgaccgc ggagattgcc tcggtgattg ctgaaggcgc gttccactat
    18061 ttggatcgcc ccgtcaagcg tattggcgca atgcacgtcc cgattccttt ttctcctccc
    18121 ctcgaggatg taaccgtgcc aaccgagaaa atggtattcg atgcggcccg cgcaatgtgt
    18181 agcggttcct aaggcctcgt cccgcatcta gaacaggaga actgaaatgg cactaccaac
    18241 ccacggaacg atggcagttg attgggagca gcggattgat ttcgaccgtt tgcgccgaga
    18301 gcgccttgcc cgcataaaaa gcctcctcga aaaatctgaa ctcggcgcgc tgctgtgctt
    18361 cgatatgaac aatgtgcgct acatcacggc gacgcacatt ggcacctggg cccaggacaa
    18421 gaacagccgc ttcacgctct tatcgcgggg agccgagccg atcgtttggg attttggttc
    18481 agccgcaaag caccatcagt tgcactgccc ttggctcggg gagcgctcgc gcgccggaat
    18541 agcgctgttg cgtggtgcca tgtccccgga aatggggcgc gccgaggacg ttgccaaaaa
    18601 aatcaaggtt gaactcgaaa aactcggtct cgaaaaagag ccgctcggcg tggatatcat
    18661 cgagccacca attcttttcg cccttcaaaa gcaaggcatt cgcatcgttg acggccaaca
    18721 aatgttgtcg gatgcccgcg tcatcaaaac acaagatgaa attgccctgc tgaaccattc
    18781 agcgatgatg gtcgatgccg cctatgac
//
LOCUS       DATNDT010000098         6030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2367505, whole genome shotgun sequence.
ACCESSION   DATNDT010000098 DATNDT010000000
VERSION     DATNDT010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6030
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2367505"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="VN788_02920"
     CDS             <1..1129
                     /locus_tag="VN788_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19516.1"
                     /translation="NLNTKEKRIMPIPYRADHGGSFLRPIELLEARANGTDPGRLRAL
                     EDQQILRLLAKQKELGFEIFTDGELRRGNFMSGFTDAVEGFDLEGTIARDWDASQGKP
                     KVSAVMGIVNKKLRQVRPLTGGELPFLKKNSPGDIKMTLPSPTQFPAIAFKRGVTDKV
                     YKDHSELLWDIVEIMKKDIGTLATDGVKYIQIDAPRYSYYMDPKWRDWIQKEIHMDPE
                     SALDEAIKADNACLEAARKRKPGVTLAIHLCRGNNRSHWYAQGGYDAVAEKLFGTLDV
                     DRFHLEYDDERSGTFEPLRFVPKSKIVVLGIVSTKLPQLEKSEDVIKRINEAAKYFPL
                     ENLTLSPQCGFASTAEGNLLSEDEQWAKMKLVVDTAQRVWS"
     gene            1448..2779
                     /locus_tag="VN788_02925"
     CDS             1448..2779
                     /locus_tag="VN788_02925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19517.1"
                     /translation="MRVHLDWLQYKQNFREPVLVLKTTGEQPEGPASIEIRVDSRQVT
                     PGSLRDPLKRALAVSNPGSAEGPDRIYLEEFKSFRSSIAWEFNRLYWQRLKDWEHATG
                     KSYEKALPGGKSDGNRPEAISDSVSDLWALLQNLDSKNQLPAEIFLLEIGVGMGIRAG
                     MFLDLFRDLDKEKGTNYYPRIRFLLGDYSLATLERSRPAVQSHVDLCSFIALDALDPL
                     RTLSFLRHKILYVHLTNTYDNLPDEEVVWRNGRVYWVQVRAYISAAEAARIGRDCDVA
                     EADIPRAVKRLLEVGAEALPDRAKGMRFWMEIWNAMRTEERLENMLDLPDASFPAGLS
                     IPQMEDILKGAPDDLRFHLSSGALESFRNTLPLLHPRGYLQVQDIFVTDLNDYGMGFY
                     GPGKLEGSIVSWVNGALLREVAERGGYDLHFAPYTYRPGSKTSILYTTRRE"
     gene            2785..3786
                     /locus_tag="VN788_02930"
     CDS             2785..3786
                     /locus_tag="VN788_02930"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="catalyzes the formation of methionine from
                     methylcobalamin and homocysteine; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXU19518.1"
                     /translation="MTLPILPTTMVGSYSMPGWLERLKTEYFARRISRVELDEIHDTA
                     VKAAIKDQEIAGLDIVTDGELRRDNMIDYFVERLPGVQVDRSSKKFYYDFYDSEVIGK
                     IPMAAIGLVDDFRFLLANTERETKICITGPHSLTKRIRNKHYPSEEALATDIARVMNM
                     ELKELVKAGAKFIQIDEPYYSGFPEDINWGVKTLNALVEGVNAKIAVHICYGNRYGKP
                     SWEGSYRYLFPAILDAKVQQLSLEFARRGGEDLELFREFSPTFELGAGVIDVKDHAVE
                     TPDIVAERIRKTMKVVPAEKLYILPDCGLFHLPRDIAFAKLRAMVAGTRIVRKELGR"
     gene            complement(3823..4947)
                     /locus_tag="VN788_02935"
     CDS             complement(3823..4947)
                     /locus_tag="VN788_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXU19519.1"
                     /translation="MPKTPEEWLPVSAPPEKTKGVLKLFLGYAPGVGKTYGMLSEAIR
                     RHARGEDIVIGFVETHGRKGIAELLPEMETVPRRKLEYKGTVFEEMDVDAILARRPQV
                     ALADELAHTNIPGSKHRKRYEDVLELLEAKIDVLSTVNIQHIESIAPTVHTITGVVVR
                     ETVPDWVLQVAEETVMVDLTPEALANRMRRGDIYRQEKVEQSLRNFFRRGNLIALREL
                     ALRQVAEQVDRSLSSYMDEKDIKENWAVRERLAVCISANPRAQYLLARAARMARRMDA
                     ELYVVHVDIGEHLNARDEKTLAANLQFAESLGATSVRLKGANVPDAAAKFVREKHVTQ
                     MIFGRTATEGWRKLRYLNSINRFLRDAPAVDVHIVTQEPD"
     gene            4996..>6030
                     /locus_tag="VN788_02940"
     CDS             4996..>6030
                     /locus_tag="VN788_02940"
                     /inference="COORDINATES: protein motif:HMM:NF024884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HXU19520.1"
                     /translation="MRAPILNHVLQFGCAFGILAAITFLYQRFLPVNATTAALTFLLA
                     ILAVSTMWGMAVSVPMSIGAMLAFNYFFLFPAGTLRIADPQNWVALVAFLVVAIIGSH
                     LSSRARQRAADASARQRDIQRLYSFSQGLLESRNAMELVNRIPAQVVDAFEVGAAALF
                     VADKQKVYRSGPVIPHLDTERLKAILARDEPLTDTANSVCFVPVRLGVQPIGSIGISG
                     TILPRQTLEAIGTLIAIAMEHARAVEQVGQTEAAREAEKLRTALLDAVAHALRTPLTS
                     IKVSVTNMLSNPSLSDAQRLELLTIINEESDRLNRLVGEAAEMARLDAGEVELRIEPQ
                     PIDHVITAALE"
BASE COUNT         1314 a         1608 c         1832 g         1276 t
ORIGIN      
        1 aaatctgaat acgaaagaga agcgaatcat gccgatacca tacagagccg atcatggcgg
       61 aagttttctg cggcctatcg aattgctgga agcgcgggcc aacggaactg acccgggccg
      121 tctgcgcgcc ctggaagatc aacaaattct gaggctgctc gcgaaacaga aggaactggg
      181 ctttgagatt ttcacggatg gcgagctgcg gcggggcaat ttcatgagcg gttttaccga
      241 cgcggtggaa gggttcgacc tcgaaggcac gatcgcgcga gactgggacg cctcgcaggg
      301 caagcccaag gtgagcgcgg tgatgggcat cgtcaataag aagcttcggc aggtgcggcc
      361 actgacgggt ggcgaacttc cctttctgaa aaaaaacagt cccggcgata tcaagatgac
      421 gctgcccagc ccgacgcagt ttccggcgat cgcgttcaag cgcggcgtga ccgacaaggt
      481 gtacaaagac cattcggaac tgttgtggga catcgtcgaa atcatgaaga aagatatcgg
      541 tacccttgcg actgacggcg tgaaatatat ccagattgat gcgccgcgct acagctacta
      601 catggacccg aaatggcggg attggattca gaaagagatt catatggacc cggaatccgc
      661 gcttgacgaa gccatcaagg cggacaatgc gtgcctggag gcggcgcgca aacgcaagcc
      721 cggggtgacc ctggcaatcc atttgtgccg cggcaacaac cggagccact ggtatgccca
      781 agggggctac gatgccgtgg cggagaagct tttcggaact ctggacgtag atcgtttcca
      841 cctggagtac gacgacgagc gctccgggac gttcgagccg ctgcggtttg ttcccaagag
      901 caaaatcgtt gttctcggga tcgtcagcac caagctgccg cagcttgaaa agagcgagga
      961 cgtaatcaag cgaatcaacg aggcggccaa gtattttccg ctggagaacc tgacgctgag
     1021 tccgcagtgc gggtttgctt cgaccgcgga aggcaacctg ctgagtgaag acgagcagtg
     1081 ggcgaaaatg aaattggtgg tggacaccgc tcaacgtgtt tggagctaaa gaacccagcg
     1141 cccgcctgcc ggtagtgact cagtttgcaa gcagcacgac cgctcgtgcc gattgcctcg
     1201 actcacgcgc aggcaagaat ggctgcgcca caaaatggcg gggttagtta aaaacctaaa
     1261 ccgcgctctt ccaaaaccca aggttgtcac ataaactctt aagccctaga gctacgccaa
     1321 caggaccttg atccttcatc tcaatcacac gcgacgcgat tcgatcatcg ctaccacaaa
     1381 tgaggtggta agcacgcttc tatcattggt tgatgaagga tgtttgcagc cggagcgttt
     1441 tgcgcggatg cgagttcatc tggattggct gcagtacaaa caaaatttcc gcgagccggt
     1501 tctggtgttg aaaacgaccg gggagcagcc cgaaggcccg gcgtcgatcg agattcgcgt
     1561 cgattcccgg caagtgactc cgggatcgct gcgcgacccg ctgaaacgag cgctcgcagt
     1621 ttcaaatcct ggatccgcgg aagggccgga ccgaatttat ttggaagaat tcaaatcgtt
     1681 ccgcagcagc attgcctggg aatttaaccg tctttactgg cagcgattga aagattggga
     1741 gcacgcgacg ggcaagagct acgagaaagc gcttccggga ggcaagtccg acgggaatcg
     1801 tccggaggca atcagcgatt ccgtttccga tctgtgggcg ctgcttcaga acctcgacag
     1861 taagaaccaa ttgccagcgg agattttcct actggaaatt ggcgtaggaa tgggaattag
     1921 agccgggatg tttctcgatc tctttcgcga tctcgataag gaaaagggca cgaactatta
     1981 tccgcgcata cgctttcttt tgggcgatta ctcgcttgcc acgctggagc gttccaggcc
     2041 ggctgttcaa agccacgtgg atctgtgcag ctttatcgca ctggatgcgc tcgatccgct
     2101 gaggactctc tcatttttgc gccacaagat tttgtacgtt catttgacca acacgtacga
     2161 caatcttccg gacgaggaag tagtatggcg gaatggccgg gtctattggg tgcaagtccg
     2221 cgcgtacatt tccgcggcgg aggccgctcg catcggccgc gactgcgatg tagcggaagc
     2281 cgatatcccg agggccgtga aacgattgct cgaagtgggc gccgaagctc tccctgatcg
     2341 cgcgaagggc atgcgcttct ggatggagat ttggaatgcc atgcgaaccg aagagcggct
     2401 ggaaaacatg ttggatttgc cggacgcctc gttcccggcc gggctgagca ttccgcaaat
     2461 ggaagacatc ctgaagggcg cgccggacga tttgcgtttt catctcagct ccggtgcgct
     2521 tgaaagcttt cgcaacacgc tgccgctgct gcacccgcgc ggctaccttc aggtacagga
     2581 tattttcgtg accgatctga atgactacgg catggggttc tatggcccgg ggaaattaga
     2641 aggctccatc gtaagctggg tcaacggcgc ccttttgcgc gaggtggccg agcgcggcgg
     2701 ctacgacctg catttcgcgc cctacacgta ccggccaggc tcgaagactt cgatcctgta
     2761 tacgacaaga cgggagtaag gcccatgact cttcccattc ttcccaccac gatggtcggc
     2821 agctactcga tgccgggctg gctggaacgg ctgaaaacgg aatatttcgc tcgacggatt
     2881 agccgcgtgg aactcgacga gatccacgac acggccgtga aagcggccat caaggaccag
     2941 gaaattgcag ggctcgatat tgtgacggat ggcgagcttc gccgcgacaa tatgatcgac
     3001 tacttcgtcg agagacttcc gggcgtgcag gtggatcggt cgtccaagaa attttattac
     3061 gatttttacg acagtgaggt aatcgggaag atccccatgg ccgctatcgg tctggtagac
     3121 gacttccgct tcctgcttgc caataccgag cgggaaacta agatctgcat caccggcccg
     3181 cactcgctga cgaagcgcat tcgcaacaag cactacccga gcgaggaggc gctggccacg
     3241 gatatcgcgc gggtgatgaa tatggaactg aaggaactgg tgaaggcggg cgctaagttt
     3301 attcagatcg atgagccgta ctattcaggt tttcccgaag acattaactg gggcgtgaaa
     3361 acgctgaatg cgctggtgga aggcgtgaac gcgaagatcg ccgtccatat ttgctacggc
     3421 aaccgctacg ggaagccgtc gtgggagggc agttaccgtt atcttttccc agcgattttg
     3481 gatgcgaagg tccagcaact gagccttgaa ttcgcgcggc gcggcggcga ggatttggag
     3541 ctgttccggg aattttcgcc aacgttcgaa ttgggcgccg gagtgattga cgtgaaggat
     3601 cacgccgtgg agacgcccga tatcgttgcg gaacgcatcc ggaaaaccat gaaggtggtg
     3661 cctgccgaga aactctacat tctgccggat tgcggcctct ttcatttgcc gcgcgacatc
     3721 gcgttcgcaa agctgcgcgc catggtggca ggcacgagga tcgttcggaa ggaactgggg
     3781 cgctaaacag ggtttgagga ttttcgggcg gcgagcggac cctcaatcgg gctcttgcgt
     3841 gacgatgtgg acatccacgg cgggggcatc gcgcaaaaaa cggttgatag aatttagata
     3901 gcggagcttg cgccatcctt cggttgctgt cctgccaaaa atcatctgag tgacgtgctt
     3961 ttcgcggacg aacttggccg cagcatcggg gacgtttgcg cctttcaaac ggacagatgt
     4021 agcacccaaa ctttcggcaa attgcaagtt tgccgcgaga gttttttcgt cgcgtgcgtt
     4081 gagatgttcg ccgatatcaa cgtggaccac gtacaattcc gcgtccatgc gtcgagccat
     4141 ccgcgcggcg cgcgccagca ggtattgcgc gcgggggtta gcgctgatgc agacggcgag
     4201 gcgttcgcga accgcccaat tttccttgat atctttttcg tccatgtagg aggagaggct
     4261 gcgatcgact tgttcggcga cctggcgcag agcgagttcg cggagggcga tcagatttcc
     4321 gcggcggaaa aaatttcgca gggactgttc gactttttcc tggcggtaaa tgtcgccgcg
     4381 tcgcatgcgg tttgcaagcg cttccggggt aaggtcaacc atcactgttt cttcggcgac
     4441 ctggagcacc cagtcgggca ctgtttcacg gaccacaacg ccggtaatgg tgtgaacggt
     4501 aggcgcgatg ctttcgatat gctgaatgtt gacggtggaa agcacgtcga ttttggcttc
     4561 gagcagctcg agtacatctt cgtaacgttt gcggtgcttg ctgccaggaa tattggtgtg
     4621 cgccagctca tccgccaacg cgacctgcgg gcgccgggcg agaatggcgt caacgtccat
     4681 ttcttcgaac accgtgccct tgtattcaag cttccggcgc gggacggttt ccatctcggg
     4741 taaaagttcc gcgattccct tccggccatg cgtctcgacg aacccaatca caatgtcttc
     4801 gccacgcgcg tggcggcgga tggcctcgct cagcatgccg taagttttgc cgacaccggg
     4861 ggcgtaaccc aaaaatagtt tgaggacgcc cttggttttc tcgggcggcg cggatacggg
     4921 tagccactct tcaggtgtct tcggcacgag gtattctctt tagggtgcct tcgcgagcta
     4981 cgatagaatg gcgcgatgcg agcgccaatc ctaaaccacg tcctgcaatt cggatgcgct
     5041 tttggaattc tcgccgcaat aacatttttg taccagcggt tcctcccggt gaatgccacg
     5101 accgcggcgc tcacgtttct gctggctatt ttagcggttt cgacgatgtg gggcatggcc
     5161 gtttccgtgc caatgtcgat cggggcgatg ctggcattca attacttttt tttgtttcct
     5221 gcagggacgc tcagaatcgc cgatccacag aattgggtgg cgctggtggc cttcctggtg
     5281 gtggcgatta tcgggagcca tctctcgtcg cgcgcgcgcc agcgggcagc ggatgcttca
     5341 gcgcggcagc gcgatattca gaggctgtat tcgttcagcc aaggattgct cgaatcgcgc
     5401 aacgcgatgg aactggtgaa tcggattccc gcgcaagtgg tggatgcctt tgaggtggga
     5461 gcagccgcgc tctttgttgc cgataaacag aaggtgtatc gctccggccc ggtaattccg
     5521 cacctggata ccgagcgcct gaaagcaatc ctggcgcgcg acgaaccact gacggatacc
     5581 gccaatagtg tttgctttgt cccagtgcgg ctgggagtgc agccaatcgg cagcattgga
     5641 atttcaggca cgatccttcc gcgccaaact cttgaagcca tcgggacact gatagcaatt
     5701 gccatggagc atgcgcgggc cgtcgagcag gtgggccaga cggaagccgc gcgagaagcc
     5761 gaaaaactgc ggaccgcgct gctcgacgca gtagcgcatg cgctgcgaac gccgctgacc
     5821 tcaatcaaag tttccgttac gaacatgctg tccaacccgt ccttaagcga cgcgcaaagg
     5881 cttgaactgc tgaccatcat taacgaggaa agcgatcgtt tgaatcgcct ggtcggtgag
     5941 gcggcggaaa tggcgcggct cgatgccggt gaggtcgagt tgagaatcga gccccaaccg
     6001 atcgatcacg taatcactgc ggcgctggag
//
LOCUS       DATNDT010000099        12565 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_901611, whole genome shotgun sequence.
ACCESSION   DATNDT010000099 DATNDT010000000
VERSION     DATNDT010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12565
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901611"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VN788_02945"
     CDS             <1..295
                     /locus_tag="VN788_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HXU19521.1"
                     /translation="MTDDIAERGRKIGGATIRSISDITRHQRLKDNNAEVVSPKDMLA
                     ELSADNQHLTRSLRSTHELCEKYSDVATASLIEVWIDQTERRTWFLSEIVRDL"
     gene            complement(452..1378)
                     /locus_tag="VN788_02950"
     CDS             complement(452..1378)
                     /locus_tag="VN788_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXU19522.1"
                     /translation="MTITSRDFDYVRKLALESSALAIGPGKEYLVESRLNVLASSEGF
                     SSLHHLIQGLHLDRSGNLKRKVVEALMTHETTFFRDVRPFETLRRVIVPAMIMQRASE
                     RSLNIWCAGCSAGQETYSIAMLLLENFPALAGWTVYLMGSDISKRVIDRARSGRYTQL
                     EVNRGLPANLLVKYFQKAKGEWQINGSVRRMAAFQEINLAGAWPRLPPMDIIFLRNVL
                     IYFDAETRTDILRRAHRLLNPDGYLFLGGSETTAGATDKFERLPDERSTVFRPTEHHD
                     ILGKSKGQSIEINAPRNPPHTLAAIVRNTRAT"
     gene            complement(1375..2505)
                     /gene="cheB"
                     /locus_tag="VN788_02955"
     CDS             complement(1375..2505)
                     /gene="cheB"
                     /locus_tag="VN788_02955"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017313321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HXU19523.1"
                     /translation="MPKMRVLVVDDSVVVRRVLANVLSSDPAIDVVGTAPNGRIALAK
                     IAQATPDLVTLDVEMPEMGGLETLAALRKTNPLLPVIMFSAFIEQGSPNAANALAFGS
                     VECVTKPVGGAVDSASTAIRNYLIPKIKGHLPHEVVPAAPVPHADSVRSVFPGKSFPS
                     QTDHPVPRVDVVVIGASTGGPSALEKLLAFLPDDFPVPILIVQHMPAGFTALLANRLA
                     AKCRISVAELRADPLLCPGHAWLAAGGRHMVVKVMNGEVHARIFDGPPENSCRPSADV
                     LFRSAAEVFKEHVLAVVMTGMGQDGLRGCEKIREAGGQVLAQDQASSVVWGMPGLVAN
                     AGLVNRVVGLDELGREILRRVMFLRPRSMASHRDSPRARSQS"
     gene            complement(2510..2881)
                     /locus_tag="VN788_02960"
     CDS             complement(2510..2881)
                     /locus_tag="VN788_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19524.1"
                     /translation="MNALVIDDSSTMRLILKRFLAKMGFHVVEATNGREALERLREMG
                     QTDLVLVDWNMPEMNGVDFVRSVRADRSFDSLPLVMVTTNTELEHVAEALEAGANEYV
                     MKPFTMDVIREKLSLLGFLEA"
     gene            complement(2919..4658)
                     /locus_tag="VN788_02965"
     CDS             complement(2919..4658)
                     /locus_tag="VN788_02965"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXU19525.1"
                     /translation="MSMTIREKLYYGYGTVVAMLVIIFAINIAATVRERSARAGAASA
                     LENVHLVETIRFQIMEDRVYLLNYLLSGDLREENQMTDGVRDLVQMFHKLVPDSVSEK
                     MADSLRQVDNNEERWNNEFAKPLIAKREQVDSGDTTVADLQILYLQKDPNSWITKSSA
                     LLDQAEEGIRKAQDKSTASVSTATAISAGVSTLGAIVAILLGLTIAYRSAKSITQPLK
                     ETVLALDDIAHGEGDLTRRVGESRKDELGDLGRCFNIFIGKLEDLVAQIADSTQGVAG
                     SSEQLFAVSRQMGSNAEETSAQASVVATAAEQVTVSLNAVATATEEMTASIGEIAKNA
                     GEAAEVSTLAVRKTDAANATMTRLGQSSAEIGSVVKLITSIAQQTKLLALNATIEAAR
                     AGAAGKGFAVVANEVKELANETAKATDQITQKIQMIQHDTGASMEALADISGVIARMN
                     DISSTIASAVEEQTATTNEIARNVSDAAKGGSLVTNNIESVAQAAKGTAEGAQETLGA
                     AGELAKMAAELQKMVAQFKYHSRSGSANWDASESQETFRPPWREPARADQKPELFHAS
                     VDNPAARKRLYPA"
     gene            complement(4704..4943)
                     /locus_tag="VN788_02970"
     CDS             complement(4704..4943)
                     /locus_tag="VN788_02970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXU19526.1"
                     /translation="MIKTVKPEYPALADKMHMTGSVKIAVIISPDGKVKSIRVVGGHP
                     LLIPAATDAAKRWEFAPDRRETSQVIEFKFDAADH"
     gene            complement(5048..5518)
                     /locus_tag="VN788_02975"
     CDS             complement(5048..5518)
                     /locus_tag="VN788_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXU19527.1"
                     /translation="MPEGRQFCSFIVDDLLLGADVRNVQEVVRDLEITAVPSAPRAVR
                     GLANFRGQVLEVIDLRCYLGINDPALESASFYVIFRTPEGPISLLVDEVGSVMEVGEE
                     DCSSVAPETLKGPMREIISVVYQLSDRLLPILDMDRLLSEVVAAEHRTIPGDAA"
     gene            complement(5511..7823)
                     /locus_tag="VN788_02980"
     CDS             complement(5511..7823)
                     /locus_tag="VN788_02980"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXU19528.1"
                     /translation="MNEMDIIIKEFLVESNENLDQLDRDLVQLEREPTSRELLGRIFR
                     TVHSIKGATGFLGFGKLGGVAHAGENLLTRLRDRNLVITPAITTGLLSLVDAIRGMLS
                     QIAENGTEGGGDYARLVETLTHLQEVAHAGKDPPATARPESSIASQPVQSENSADERI
                     REASRPGVTQKADPNGETHTMEAQEQQETRAGKFASSTLRVNVEQLDSLMELVGELVL
                     ARNHILEVASRQREPDFVAVSQHLDSITSEIQQRIAQLRMQPINTVWGKFPRLVRDLA
                     VQCGKRVRLVMHGEETELDKMILEMIQDPLTHLVRNAVDHGIESPEVRIASNKPEEGT
                     VTLRAFHENGQVNIEISDDGGGIDVERVKETAVKKKLITAETAALLSARDLLQLIFLP
                     GLSTAKNVTDVSGRGVGMDVVKSNIERIGGALDIQTRKGAGTTIKIAIPLTLAIVPAI
                     LITSDGESYAIPQANVVELVRLSDGAKEVRIESVREASFYRLRSELLQCASLENAVWP
                     NRLSGERSRPEQPAAGGVMVVLQAQDRRFGLLVDEANDTQEIVVRSIGKRLKHIPVYA
                     GATILGDGRVALILDVPGLAARVCTRRENGRPVPLIQPSESSTCVAESDALLIFEHRE
                     SKRFALPISGIARLEQLPRSRIEATGGQRLVQYDGAILPVFGISDDLTANQPSHGSEN
                     TWVTTDENIELVICQHQGRSLALAVNHIVDIVEEGVTNVGESIADGASASRIIDGRVT
                     RVLDLEELLTRAEPSISQAPALVKIGARDA"
     gene            complement(7863..9998)
                     /locus_tag="VN788_02985"
     CDS             complement(7863..9998)
                     /locus_tag="VN788_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXU19529.1"
                     /translation="MASALANLGLADERGIAVAIAKSLQLESLSDELPDVSPDVSALL
                     PADFCRKRQIVPLSLKGKALRLALIDPMDYTTTQDVEFRTGKRVTAVVATEVSIRALL
                     DRVYPAQDEADQVFSAGELEGEVESVADAEFEIVDPAKLAKDTKMPPVVRLVNLILSG
                     AAKEGASDIHMEPKESHLQVRYRMDGLLRDVIKIPKNQQDATISRMKIISGMDIADRR
                     RPQDGRSRLRFEGKRIDLRVSTLPTHFGEKVVIRLLDSRRAQMAMDQIGLTGENLHSF
                     QMMLSRPQGMMLVTGPTGSGKSSTLYTALNWVKSPTKNIITVEDPVEYQLDGVNQVQI
                     NPKAGITFAAGLRSILRQDPNVILVGEIRDQETAGIALEAAQTGHLLLSTLHTNDAPG
                     SVTRLLDLGIEPFLISSAVIGILAQRLVRKPCPRCSEPQEPSADAIEKAGGASRLPAD
                     AKWVAGRGCGECNHSGAKGRMAIHELLVVNEDVRELITRRASEHAIRKAARDGGMRTL
                     LEDGIVKAAAGLTTLEEVVRVVAADDAAVLKEDATKPAASSPVAHKIPEEEIQIDAEE
                     VPDTPISDASGAESRAQQRVLVVEDSRTILAVVKYFLELEGFAVLEAKDGQTGLEVAK
                     RDHPDVIVTDCNMPGMDGMAMVRKIRAHPPIRDIAVIMLTSENSVEKEEEALVAGADD
                     FILKPVEPRRLAARVKSVLARKPARRASN"
     gene            complement(10346..11017)
                     /gene="deoC"
                     /locus_tag="VN788_02990"
     CDS             complement(10346..11017)
                     /gene="deoC"
                     /locus_tag="VN788_02990"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HXU19530.1"
                     /translation="MASWQNLAAIIDHTNLRPEATPRDITRLCDEAREIGFGAVMVNP
                     CYVALASANLQNGGVKVGAVVGFPLGATLTSVKVFETQEALALGAREIDMVLNIGALK
                     AGNRELVLSDIASMAETAHQRKALLKVILETGLLTDEEKLAACTISEKAGADFVKTCT
                     GFLGGIATIEDVALMRSAVKIGVKASGGIRTASDARAMVEAGANRLGTSSGVNIIREL
                     RVHGE"
     gene            11176..11628
                     /locus_tag="VN788_02995"
     CDS             11176..11628
                     /locus_tag="VN788_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008182373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXU19531.1"
                     /translation="MARAIQLSIENVRAARGGPFGAVVVRGGKILAEGVNCVTLANDP
                     TAHAEVVAIREACRKLQLFELKGCEIYTSSEPCPMCLGAIYWARLDRAYFGNTTADAS
                     KAGFDDSLIYFEISRPHSQRKIPVIQLMRDEALEAFRVWRQQPNKLSY"
     gene            11918..12382
                     /locus_tag="VN788_03000"
     CDS             11918..12382
                     /locus_tag="VN788_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013043487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXU19532.1"
                     /translation="MAKETEARLANLGIILPAVPQAGGNYIPAKTVGHIVYLASAIST
                     SSEGVITGTVGLNRTIDEGYAAARSCALTQLAVLKRHLGSFDPVKSIISANGYVNAVP
                     GFTDSPKVINGASDLLVEIFGDSGRHVPAAIGVSALPRNALVELQMTVELEA"
BASE COUNT         2553 a         3732 c         3376 g         2904 t
ORIGIN      
        1 gatgaccgac gatattgccg agcgcggccg taagatcggt ggggcgacga ttcgttccat
       61 cagcgatatc acgcggcatc aacgtttgaa ggataacaac gcggaagttg taagcccgaa
      121 agatatgctg gccgagcttt cagccgacaa ccagcacctc acgcgctctc tgcgctcgac
      181 gcacgagctc tgcgaaaagt acagcgacgt cgcaaccgcg agtttgattg aggtttggat
      241 cgaccagacg gagcggcgga cctggttctt gtcggaaatc gtgcgcgatc tttagagcgc
      301 ggtgctcccg cacgacgcgt cgtggcttag ctccacttgt cgatagagac gctgaggccg
      361 taactcaagc tgccgcccta gaagaacgcg ttggtttgtc cactagcttg gcgaagtcat
      421 ttaagaactt cctcatccaa gatggcgaca tttatgttgc tcgagtattc cggacgattg
      481 cggccaacgt gtgaggtggg ttacgcggag cattaatctc gatcgattgt cctttcgatt
      541 ttccgaggat gtcgtggtgc tccgtgggcc gaaatacggt ggaccgctcg tcgggtagcc
      601 gttcaaattt gtcggtcgcg ccagccgttg tctcggagcc gccgagaaag agatagccat
      661 ccgggttcag cagacggtgc gctcgtcgaa gaatatctgt tctagtttct gcgtcgaagt
      721 aaatcagtac gttccgcaga aatataatgt ccataggcgg taaacgaggc caagcgccag
      781 cgagatttat ttcctgaaaa gcggccattc gtcgcacgct tccgttgatt tgccattcgc
      841 ctttcgcttt ctgaaagtat ttcacgagca agttagctgg cagaccgcga ttgacttcga
      901 gttgcgtata gcgcccggaa cgcgcgcggt cgatcacccg cttggatatg tcgctgccca
      961 tcaagtacac cgtccatcct gcgagagcgg gaaaattttc gagcagcaac atggcgatgc
     1021 tataagtttc ctggccggcc gagcaccccg cgcaccatat gttgagcgaa cgctccgaag
     1081 cgcgctgcat gatcatggcg gggacgatga ccctgcgcag cgtttcgaag ggccgcacat
     1141 cgcggaagaa agtggtctca tgggtcatca gcgcttcgac gactttgcgc ttcaagtttc
     1201 cagatcggtc gagatgcaga ccttggatca gatgatgcaa ggacgaaaag ccctcactgg
     1261 aggccagcac gttcaggcgc gactccacca aatactcctt gccgggccct atcgccagtg
     1321 ccgaactttc gagggctagc ttccgaacat aatcgaagtc gcggcttgta attgtcatga
     1381 ttggcttcta gcgcgcgggc tgtctcgatg tgacgccatg ctgcgtgggc gcaagaacat
     1441 cacccggcgc aatatctcgc gtccgagctc atcgagcccc acaacacggt taaccaggcc
     1501 cgcatttgca accaaccccg gcatgcccca taccacggag cttgcttggt cttgagcaag
     1561 cacttggccg ccagcctcac ggattttttc gcaacctcga aggccgtcct gacccatccc
     1621 cgtcattact acggcgagca cgtgttcttt gaacacctca gccgcggaac ggaagaggac
     1681 atccgctgag gggcggcagg aattttccgg cggtccatcg aatatcctgg cgtggacctc
     1741 gccgttcata actttcacaa ccatgtgcct gccgccggcc gccagccacg catgaccagg
     1801 gcacaaaagc ggatcagcgc gcagttccgc cactgaaatc ctgcacttcg ccgcgagccg
     1861 attcgcaagt agtgcagtga agccggcagg catatgctgc acgatgagaa ttggaacagg
     1921 gaagtcgtcc ggcagaaatg ccaagagctt ttcgagggcg ctaggtccac ccgtcgaggc
     1981 gccaataacc acaacgtcga cgcggggcac gggatggtca gtctgcgagg gaaatgactt
     2041 gccaggaaaa acgctccgga ctgaatcggc gtgagggaca ggcgcagcgg ggaccacctc
     2101 atgcggtaaa tgtcctttga ttttgggaat gagataattg cgaattgccg tggaggcgga
     2161 atcgacggct cctccgacgg gtttggttac acactcgacg ctgccgaagg cgagtgcgtt
     2221 cgcggcattg ggcgatcctt gctcaatgaa agcgctaaac atgataaccg gcagcagcgg
     2281 attcgtttta cgcagcgccg caagggtttc caggccaccc atttccggca tttcaacgtc
     2341 gagtgttacc aaatccggcg tagcctgtgc gattttggcg agcgcgattc gtccattcgg
     2401 cgcggtgccg acgacatcga ttgcagggtc gcttgagagc acgttcgcga ggacacggcg
     2461 caccaccacc gaatcgtcca ccacaagaac acgcattttg ggcataaaat caggcttcca
     2521 gaaatcccag aaggctcagt ttttcgcgaa tcacatccat cgtaaatggt ttcatcacgt
     2581 attcgtttgc accggcttct aatgcttcag ccacgtgctc caactcggtg ttggtcgtga
     2641 ccatcaccag cggaaggcta tcgaaactgc gatctgcgcg gaccgatcgc acaaaatcca
     2701 ccccgttcat ttccggcatg ttccaatcca ccagtacgag gtcggtctgg cccatttccc
     2761 gaagccgctc caatgcttcg cgtccattcg tcgcctccac aacgtggaag cccatcttgg
     2821 ccaggaaacg tttcagaatc agccgcattg tgctcgagtc gtctatcacc agggcgttca
     2881 ccgggactcc ttcgtcgtcg ctcgccgaat cgttatgctt atgcgggata taaccgcttt
     2941 cgcgcagccg gattgtccac gctggcgtga aatagctcag gcttttggtc cgcgcgagct
     3001 ggttccctcc agggcggccg aaaagtttcc tggctttcgg atgcgtccca attcgcgctg
     3061 ccgcttcggg agtgatactt aaactgagcg accatttttt gaagttccgc agccattttt
     3121 gcaagttcgc ccgcagctcc cagcgtttcc tgcgctccct cagcggttcc ctttgcggcc
     3181 tgcgccacag actcaatgtt gttggtaact agcgaaccac ccttggccgc gtctgaaacg
     3241 ttccgcgcaa tctcattagt cgtcgctgtc tgttcttcga ctgcactagc gatcgtgctg
     3301 gatatatcgt tcatgcgcgc gataacgccg ctgatatccg cgagcgcttc catggatgct
     3361 ccggtatcgt gctggatcat ctgaattttc tgggtaatct gatcggttgc tttggcggtc
     3421 tcgttcgcaa gttctttcac ttcatttgcc actactgcaa atcccttgcc ggcggcgccg
     3481 gcccgcgctg cttcgattgt ggcgttcagc gccagcagtt tggtttgctg ggcgattgaa
     3541 gtgatgagct taacgacact tcctatctcc gcgctcgact ggccaagcct ggtcatcgtt
     3601 gcattcgccg catccgtctt gcgaacagcc aaagtagaga cttcggccgc ctcaccggcg
     3661 tttttggcaa tctcaccaat gctcgcggtc atctcctcgg ttgcggtagc caccgcgttc
     3721 aaactgacag ttacctgttc ggccgccgtc gcaaccacgc tcgcctgggc cgacgtttcc
     3781 tcggcgttcg atcccatctg acgactcacc gcgaagagct gttcagatga acccgctact
     3841 ccttgggtgc tatcggcgat ttgtgcgact aggtcttcca gttttccgat gaaaatattg
     3901 aaacatcttc cgaggtcacc gagctcatcc ttgcggcttt ctccaacgcg gcgagtaaga
     3961 tcgccttcac catgagcaat gtcgtcgaga gcgaggactg tttccttcaa aggctgcgta
     4021 atggatttcg cggagcgata cgcgatcgtg agccccagca gaatggcgac tatcgcccca
     4081 agggtgctaa caccggcact gatcgcggtg gcagtggaga ctgaagcggt ggatttatcc
     4141 tgcgccttgc ggatgccttc ttcggcttga tcgagcaaag cgctggattt ggttatccaa
     4201 ctgttcgggt ctttctgcag atagagaatc tgcaaatcag caacggttgt atctcctgaa
     4261 tcaacctgtt cgcgtttcgc gatcagcggt ttcgcgaact cgttgttcca tcgttcctcg
     4321 ttattgtcga cttggcgaag cgagtcggcc atcttttccg agacggagtc tggaactaac
     4381 ttgtgaaaca tttgtacgag atcgcggacc ccgtcagtca tctgattttc ttcgcgcaag
     4441 tcgccgctaa gcaaatagtt gagcaagtaa acccggtcct ccatgatctg gaaccgtatc
     4501 gtttccacca aatgcacatt ttccagagca gaagcagctc ccgctcgagc ggaacgttcg
     4561 cgcacagtgg ccgcgatatt gatggcgaaa attatcacca gcatcgcgac aactgtcccg
     4621 tatccgtagt agagcttctc gcgtatcgtc attgacattg agatctgccc gtggtggcgt
     4681 tgccctgcga accctggttc ttgctaatgg tcggcagcat cgaatttaaa ctcgatcacc
     4741 tgggaggttt ccctgcggtc gggcgcaaac tcccagcgct tcgcagcatc cgtagcagcg
     4801 ggaatcagaa gcggatgacc accgaccacg cgaatgcttt ttactttccc atctggcgaa
     4861 atgattaccg caattttcac ggaaccggtc atatgcattt tgtccgctaa tgccggatat
     4921 tccggcttca ccgttttgat caccttgcga tcagcctcca cctgaggcca gatgcaaggc
     4981 acacacgtcg ttgtcgctaa taggatctcc aacgataggc agcaaaagat tcgtggcagc
     5041 cttcccctca cgcggcgtct ccaggaatcg ttctatgttc ggctgcgacg acttcgctca
     5101 gcaggcggtc catatcgaga attggcagca gtctgtccga tagctggtag acaactgaga
     5161 tgatttcacg cattgggccc tttaaagttt ctggagcgac tgacgagcag tcttcttcgc
     5221 cgacctccat caccgagccc acctcgtcca ctaacaggct tataggtcct tctggtgtgc
     5281 ggaagatcac ataaaacgag gctgactcga gcgcgggatc attaataccg aggtagcatc
     5341 gaaggtcaat tacttcgagt acttggccac gaaagtttgc cagcccgcgc accgcccgcg
     5401 gcgcggatgg tactgcggtg atttcgaggt cccgcacgac ctcctggacg ttccgaacat
     5461 ccgcccccaa cagcagatca tcgacaatga atgaacagaa ctgccgcccc tcaggcatcg
     5521 cgagcaccaa ttttcacgag ggctggcgct tgcgatatgc tcggctccgc gcgcgtcaaa
     5581 agttcttcca gatcgaggac tcttgtaacg cggccgtcga ttatccgcga cgcggaagcg
     5641 ccgtcggcga tactctcgcc tacattcgtt acgccttctt ccacaatgtc aacgatatgg
     5701 ttgacagcca gcgcgagcga acggccctga tgctggcata taaccagttc aatattttcg
     5761 tccgttgtta cccaggtatt ttcgcttccg tgggacggtt gattggcggt gagatcgtcg
     5821 ctaattccaa aaaccggcag tattgcgcca tcgtattgaa cgaggcgctg ccctccggtc
     5881 gcttcgatgc gggaccgcgg cagttgctcc aatcgcgcta ttcccgagat aggcagcgcg
     5941 aaccttttgc tttcgcgatg ttcaaaaata agcagcgcgt ccgattccgc gacgcatgtg
     6001 gatgattcag atggctgaat tagcgggacg ggacgcccat tctcccgcct ggtgcacacg
     6061 cgagcagcca gcccgggcac atctagaatc agcgccaccc gaccgtcgcc aagaatcgtg
     6121 gctcccgcat aaacggggat gtgcttcagg cgctttccga tcgagcgcac gacgatctct
     6181 tgggtgtcat ttgcttcgtc taccaggaga ccgaaccgtc ggtcctgcgc ttgaagcacg
     6241 accattacac cccctgcggc gggctgttct ggtcgagacc gctcgccgct cagtctattt
     6301 ggccaaaccg cattctctag cgaggcacat tgcaataatt ccgatcttag gcggtaaaac
     6361 gacgcctccc ggacactttc gattcggact tccttcgcgc catcgctcag tcgcaccagc
     6421 tccacgacgt tagcttgcgg gatcgcgtag gattcaccgt ccgatgtgat cagaatcgcc
     6481 ggaacgatag cgagagtcag cggaatggcg atctttatgg tcgtccccgc tcctttcctg
     6541 gtctgaatat ccaaggcgcc gccgattcgc tcgatattgc tctttacaac atccatgccc
     6601 acgccgcggc cggaaacgtc cgtaacattt ttcgccgtcg agagcccggg aagaaaaatc
     6661 aattgcaata aatctctcgc gctcagcaga gccgctgttt ccgcggtgat aagcttcttt
     6721 ttcaccgctg tttctttcac gcgctccacg tcgatgccgc cgccatcatc ggatatctcg
     6781 atattcacct gcccgttttc gtggaaggca cgcaatgtga cggttccctc ttccggtttg
     6841 ttgctcgcga tgcgtacttc ggggctttcg attccgtggt caaccgcgtt gcgtaccaga
     6901 tgtgtgagcg gatcctgaat catttcaagg atcatcttgt ccagttcggt ttcctcaccg
     6961 tgcattacga ggcgcacgcg cttaccgcat tgaacggcta aatcccgcac caagcgtggg
     7021 aatttgcccc agacagtgtt aatcggctgc atgcgaagct gtgcgatccg ctgttgtatt
     7081 tctgacgtaa tcgagtcgag gtgttgagaa accgccacaa aatccggctc gcgctggcga
     7141 gaggcaactt cgaggatatg gttgcgggcg agcaccaatt caccaacaag ctccatcagg
     7201 ctgtcgagct gctcgacatt tacacgcaat gtgctgctgg cgaatttgcc ggctcgtgtt
     7261 tcttgttgct cctgtgcctc catcgtgtgg gtctcaccat tgggatcggc tttctgtgtg
     7321 acgccggggc gagatgcttc gcggatgcgc tcgtcagctg agttttcgga ctgcaccggc
     7381 tggctcgcaa tgcttgattc aggacgagcg gtcgcaggcg ggtcttttcc ggcatgcgcg
     7441 acctcttgca ggtgagtgag cgtctcaacc agccgcgcat agtcgccgcc tccctccgtc
     7501 ccattctcgg caatctgcga aagcattcct cgaatcgcgt ccaccagaga caaaagaccg
     7561 gttgtgatcg ccggagtaat aaccagattc ctatctcgca agcgggttag caggttttcc
     7621 cccgcgtgtg ccacgccgcc aagttttcca aaacctagaa acccggttgc gcccttgatg
     7681 ctgtgcacgg tgcgaaaaat tcgaccgagc aattcgcgcg aagtcggttc tcgttcgagt
     7741 tggaccaaat cccgatcgag ctggtccaaa ttctcgttac tttccaccag aaactctttg
     7801 atgatgatat ccatctcgtt catcggctat ctcctatcgc gattgcgcgg gcgtcatagc
     7861 ttttaattcg aagcccttct tgccggtttg cgtgccagga cggacttcac acgggctgcg
     7921 agtcggcgcg gctcgacggg cttcagaata aaatcgtccg ccccggcaac gagagcttcc
     7981 tcctcctttt cgacgctatt ttccgaggtg agcataatca cggcgatgtc tcgtataggc
     8041 ggatgagcgc gaattttccg caccatggcc atgccgtcca tgccaggcat gttgcaatcc
     8101 gtcacaatca cgtcagggtg atcccgcttc gcaacttcca ggcccgtctg gccgtccttc
     8161 gcctcaagaa ccgcgaaccc ctcaagttcc agaaaatatt tcacgacggc gaggatcgtg
     8221 cggctatcct caaccaccag cacgcgctgc tgtgcgcgcg attcggcccc agaggcgtca
     8281 ctgatggggg tatcaggaac ttcctcggcg tcaatctgga tctcttcttc ggggattttg
     8341 tgggccacag gcgaactggc ggctggtttg gtggcgtcct ctttcagcac agccgcatcg
     8401 tcggcggcta caacgcggac cacctcctca agcgtcgtta gacctgctgc cgcctttacg
     8461 attccgtctt ccagaagcgt acgcattcct ccatcccgcg ccgctttccg aatcgcatgt
     8521 tccgaggcac ggcgggtaat cagctcgcgc acgtcttcat tcactaccaa cagctcgtgt
     8581 atggccatcc ggccctttgc cccagagtgg ttgcattccc cgcagccgcg tcccgcgacc
     8641 cacttggcat cggctggcag ccgcgatgct ccgcctgcct tctcaatggc atccgcgctc
     8701 ggctcctgcg gctccgaaca gcgcggacac ggtttgcgca ccaatcgctg ggccagtatc
     8761 ccgatgactg cggacgaaat caggaaaggc tcgattccga gatcaagcaa gcgagtaacg
     8821 cttcccggtg cgtcattcgt gtgcagagta ctaagcagca ggtggccggt ctgagccgcc
     8881 tcgagcgcga tgcccgcagt ttcctggtct cgaatctcac ccacgaggat gacgttcgga
     8941 tcttgccgca agattgaacg taagccagcc gcaaaggtga tccctgcttt cgggttgatc
     9001 tgcacttggt tcacaccatc caactgatac tcaacagggt cttcgacagt gataatgttt
     9061 ttggtaggcg atttcaccca attcagcgcc gtgtagagag tcgagctttt gccgcttccc
     9121 gttgggcctg tgacgagcat cattccttgc gggcgcgaga gcatcatttg aaaactgtgg
     9181 agattctcac cggtcaatcc tatttgatcc attgccattt gcgcccggcg actatcgagc
     9241 agacggatta cgaccttctc gccgaagtgc gtaggcagcg tcgaaacgcg cagatcgatg
     9301 cgcttcccct cgaatctcag tcggctgcgg ccgtcctggg gccgccgccg atctgcgata
     9361 tccatcccgg agatgatttt catccgcgag atcgtcgcgt cctgctggtt tttgggaatt
     9421 tttatgacgt ctcgaagcaa gccatccatg cggtatcgca cctgcagatg gctttccttg
     9481 ggctccatgt gaatatcgct ggcgccctcc ttcgccgcgc cgcttaaaat caggtttacg
     9541 agtcgcacga ccggcggcat ttttgtatcc ttggcgagct tggcaggatc cacgatttca
     9601 aattccgcgt ccgcgacaga ctcaacttca ccttcaagtt cgccggccga aaatacttga
     9661 tctgcttcgt cttgggcggg ataaacacgg tctagcaatg ctcggattga aacctcggtc
     9721 gccacaacgg cggtcacccg ctttcccgtt cgaaactcca cgtcttgcgt ggtcgtgtag
     9781 tccattggat cgatgagagc cagccgaaga gcttttcctt tcaggctcaa gggcacaatt
     9841 tgcctttttc ggcaaaaatc agctggtagc aaggccgata cgtccggaga cacatccggc
     9901 agttcgtcgc taagggattc gagttgcaag ctcttcgcta tggccacggc tatacctcgt
     9961 tcatcggcca gtcccagatt tgcaagagcc gaggccagtc agattccgca tttctattgt
    10021 tcctcgcgtg cgcgaaaagg acggacgtat taatcaggcc cgcgtatcag aaggttgcca
    10081 atcaatgctt ggctgaccat cccgtcatcc cggatttgaa attagaattt tcttaattca
    10141 cgccggcccg ttttggcgcg tgggtatcag actgcatgcg catagccaca cgattcctcc
    10201 ttgaaaaatt gcacggagtt gacgtctatc gattacgttg gggggcacac ttgaggtggt
    10261 gagctgaact ctcagcgaaa tgggcgcgtc agtctggaac cactcgcaga atgggctgac
    10321 accgggctgc taccgggttg acgcatcact ctccgtgtac gcgcagttcc cgaataatat
    10381 tcacgccact gctggtaccg agccggttcg cgccggcctc gaccatcgcg cgtgcatcgc
    10441 ttgcggtccg aattccgcca gacgccttta cgccgatctt cacggcactc cgcatcaggg
    10501 ccacatcctc tatggtggca attccaccga gaaagcccgt gcaagtcttc acgaaatcag
    10561 ctccggcctt ttcgctgatt gtgcatgcgg cgagcttttc ttcatcggta agcaaccctg
    10621 tttccagaat tactttgagc agcgccttcc tctggtgcgc agtctcggcc atgctggcaa
    10681 tgtccgataa caccagttcg cgattgccag ccttcagcgc tccgatgttc aggaccatgt
    10741 cgatctcgcg ggccccaagc gccaacgctt cttgcgtctc aaatactttc acgctggtaa
    10801 gagtagcccc caacggaaaa ccaaccaccg ctcccacttt aaccccgcca ttctgcaaat
    10861 tcgcggatgc cagcgcgaca tagcagggat tcaccatcac cgcaccgaag ccgatctcac
    10921 gagcctcatc gcacagacgg gtgatatcgc gcggtgtcgc ttcgggccga aggtttgtgt
    10981 gatcgatgat ggcggcgagg ttctgccagc tagccatcgg agactttcgg tccaccactg
    11041 agtttcgact cggcatcgtc actagatgtt actacgctgt caccatcgcc cagcgcaaag
    11101 tcggccagcg gggctttcta ttcctgccgc gggaatcgca ccgcagtcta tactcgttga
    11161 gtgaccaacc cattcatggc gcgcgccatt cagctttcaa ttgagaatgt gcgcgcggcc
    11221 cgaggcggtc cgttcggcgc tgtcgtcgtg cgagggggaa aaattctcgc cgagggagtg
    11281 aattgcgtaa ctttagcgaa tgatcccacg gcgcacgccg aagtcgtcgc tattcgcgaa
    11341 gcctgcagaa agctgcagct cttcgaactg aagggctgcg aaatctatac gtcatccgag
    11401 ccatgtccaa tgtgcctggg cgcgatttat tgggcgcggc tcgatcgcgc ctattttggt
    11461 aatacgacgg ccgacgcttc caaagctgga ttcgacgatt ccttgattta tttcgaaatc
    11521 tcccggccgc attcccagcg caaaattccc gtgattcaac tgatgcgcga cgaagcactc
    11581 gaagcgttcc gcgtctggag acagcagccg aataaactct cctactagcg cttgccgctg
    11641 gtttttcgcc gggcgctcac cattgccgtt ttcagagcga agtccaaagg cgactcggtg
    11701 ctgaccgatc tttacctttt acgcgggatg agcacgtgat acgcagcgaa ttcgtgcgca
    11761 ttgcatcctg cctcctcgat gaaacaaaat tcggcgtccg atgcacggtc gttgcatggc
    11821 acatgctatc gcacgggctc gtcgtcgcaa tcgggaatct ggcgacgaac tgcctatgtt
    11881 agagtttcaa gtcgctttgc ggaggcagtc acattccatg gcgaaggaaa cggaagcgcg
    11941 gctcgcgaac ctgggcatta ttttgcccgc cgttccccaa gcgggaggga attacatccc
    12001 tgcgaagacc gtgggccata tcgtgtacct ggcgagtgcc atttcgacga gctccgaggg
    12061 cgtcatcacc ggcaccgtgg gtctgaatcg tacgattgat gagggctacg ctgcggcgcg
    12121 atcgtgcgcg ctcactcaat tggctgttct gaagcggcac ctcggctcgt tcgatcctgt
    12181 gaaaagcatc attagtgcaa acggctacgt gaatgccgtg ccgggcttta ccgattcgcc
    12241 gaaagtaatc aacggagctt cggacctact cgtcgaaatc ttcggcgact ccggccgtca
    12301 cgtgcccgcg gcaatcgggg tctctgcctt gccgcgaaat gcgctcgtcg agttgcagat
    12361 gaccgtcgag ctggaagcgt agaatttata aatggccaac catgtcctct ccgccgaacc
    12421 cattcactcg cgctggaatc gcgatctgcc accgcgcctc accatcgctt cgggcgacac
    12481 cgtgcgtctc gaatgtcttg acgcaagcgg agcgcaggta cgcccgggca tgacgctcga
    12541 cgaatacatc gagatcaaca ccgaa
//
LOCUS       DATNDT010000100         2997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1556948, whole genome shotgun sequence.
ACCESSION   DATNDT010000100 DATNDT010000000
VERSION     DATNDT010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1556948"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..351)
                     /locus_tag="VN788_03005"
     CDS             complement(<1..351)
                     /locus_tag="VN788_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19533.1"
                     /translation="MACFLIGFTVTTRTSYCQTEADAAGGSSQASTRSVGEGPDDPVR
                     PGLTSSDSTGYAQDYDNSLGVSLLKHIAQDQERLWTAPARVTLQDTIWLVPLGGLTAG
                     LIATDRDTSLHISNS"
     gene            complement(390..>2997)
                     /locus_tag="VN788_03010"
     CDS             complement(390..>2997)
                     /locus_tag="VN788_03010"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SLBB domain-containing protein"
                     /protein_id="HXU19534.1"
                     /translation="SGGVNPSAYSNYYPNYFSNRFRSPNQSLLQPAAMVHKPNPYKDI
                     PSLYDMYMQATPRPAAATRFGMDVFQNNSQNPYAIPMDLPAGPDYVVGPGDGLAIDLW
                     GGVSRRIYRSVDREGRISLPEVGPLLVSGKSLAEVQQNLQQVLRTQFRNISADVSLAR
                     LRTVRIYVVGDVANPGAYDVSSLSTPLNALFIAGGPTREGSLRIVRHLRGGQLVQNVD
                     LYDLLLHGVTSDIQRLQNGDSVLIPPIGSQVTVEGMVRRPAIYELKDEKSLSSVLELA
                     GGLLPTATLRHIEVQRTIAHQKQTMLSLNIPTSDGDAEVTKKLDSFQVQDGDRIRVFP
                     ISQGNEDAVYLEGHVIRPGRYSYHDDMRVTDLIPSFKDLLPEPSMQYAEIIRLNAPDF
                     RPSVQSFNLADALANPAQAPVLHAMDTVRVFSRFDFENPPTVSVLGDVRVPGTYRTSG
                     QIHLADAVHLAGGMEPDAQTDDAQVFRYMPDGKAEIFSVELNRALAGDSTQNIILEPR
                     DRLLIHKNTNAMQPAVVYIQGEVGNPGRYPLTTNMTVADLVRVGGGLKPDADTENGDL
                     TRYEYAGQGELTGQHEPIAISAALSGDPKNNAQLHNGDVVSIRQLPGWEDLGATITLK
                     GEVRHPGTYGIRPGERLSSVLERAGGFLPDAYPYGAILERVQVRDLETKEQNKLVLRV
                     KDAEGSLQAMPDSTPAQRQAKEMALGQYETTLMQLTTNAPIGRIAIRISSHIDRWKNK
                     SADFQARAGDTLIIPKRPDYVMVTGQVFNPTAVAYRPGKNVKWYLEQAGGATTTANRK
                     AIFVIRADGSIIGRREGLWTGSSLGSVLQAGDTVVVPEKAIGGGVQWANLFTAASVAS
                     SIVSTVFIATHY"
BASE COUNT          647 a          873 c          773 g          704 t
ORIGIN      
        1 gctattcgat atgtgcaggc tcgtgtctcg atccgttgca atcagcccag cggttaatcc
       61 acccaggggc acaagccaaa tggtgtcctg cagcgttact cgcgctggcg ccgtccagag
      121 ccgttcttga tcttgtgcga tgtgcttcag cagcgaaact cccaatgaat tgtcataatc
      181 ctgcgcataa cccgtcgagt ccgacgaggt cagccccggc cgcaccgggt cgtcgggtcc
      241 ctcgccgacg ctccgcgtgg aagcttggct cgatccacct gcagcatccg cctccgtttg
      301 gcaataactc gttctggttg taacagtaaa gccaattaga aaacaagcca acacaagttt
      361 tgcccaatgt tctacccttg cgccatccat cagtagtgag tagcgatgaa cacagtgctc
      421 acaatggacg aagccaccga cgcggcggta aacaggttgg cccattgcac gccaccaccg
      481 atcgcttttt ctggcactac caccgtgtcc ccagcttgta gcaccgatcc aagagagctt
      541 cccgtccaca agccttctct tcgcccgatt attgaaccgt ccgcacgaat cacgaagatg
      601 gccttccgat tcgctgtcgt tgttgctccg ccagcttgtt ccagatacca tttcacattc
      661 tttcctggcc ggtaggcaac cgccgtcgga ttgaagactt gtccagtgac catcacgtag
      721 tcgggacgct tgggtataat gagggtgtca cccgctctgg cttgaaaatc ggccgatttg
      781 tttttccaac ggtcgatgtg ggatgagata cgaatcgcga tgcgcccaat cggcgcattg
      841 gtcgtcaact gcatcagtgt agtctcgtat tggcctagag ccatttcctt tgcctgtctc
      901 tgggccgggg tgctatccgg catggcttga agacttccct cggcgtcctt cacccgtagc
      961 acgagtttgt tctgttcctt cgtctcaaga tctcgtactt ggacgcgttc aagaatggca
     1021 ccataaggat aggcgtccgg caaaaatccg cctgctcgct ccaaaacgga actcagccgc
     1081 tcaccgggcc gaatcccgta cgtaccggga tgcctcactt cgcccttaag tgtaatcgtg
     1141 gcgcctaaat cctcccagcc aggtaactga cgaatactaa ccacatcgcc gttgtggagt
     1201 tgcgcgttat tttttggatc gcccgacagg gccgcagaaa tagctatcgg ttcatgctgt
     1261 ccagtcagct cgccttgtcc cgcatattcg taccgcgtca aatctccatt ttccgtatcg
     1321 gcatccggct ttaggccgcc ccccacgcgg accagatcgg ccacggtcat attcgttgtc
     1381 aaaggatagc ggcccggatt tccaacttcg ccttgaatgt acacaacagc gggctgcatg
     1441 gcattggtgt ttttatgtat gagcagccgg tccctcggtt ctagaatgat attttgagtt
     1501 gagtctccgg caagggctcg atttagctct acgctgaaaa tttcggcttt gccgtcgggc
     1561 atgtaccgga atacttgcgc gtcatccgtc tgcgcatcgg gctccattcc tccggccaag
     1621 tggacggcgt cggccagatg aatctgcccg gaagtccggt acgtgcccgg aactctaacg
     1681 tcacccagca cagataccgt cggaggattt tcaaaatcga accggctgaa aactcgaacc
     1741 gtatccatag catgaagaac cggtgcttgc gccgggtttg ccagcgcatc cgcaaggttg
     1801 aaactctgca cgctaggacg gaaatccggc gcgttgaggc gaattatttc cgcgtattgc
     1861 atcgaaggtt ccggtagcaa atccttgaat gatgggatca ggtcggtcac tcgcatgtcg
     1921 tcgtgatacg aatagcggcc gggacgaatc acgtgaccct cgagatacac tgcgtcttcg
     1981 ttgccttggc ttataggaaa tactcgaatg cgatcgccgt cttgtacctg aaaggagtcc
     2041 agctttttcg taacttcagc atcaccgtcc gatgttggaa tattaaggct gagcatcgtt
     2101 tgtttctggt gagcaatggt tcgctgcact tcaatatgcc gcaacgtagc tgttggaagc
     2161 aggcccccgg ccagttccag gacactggac aagctcttct cgtccttcaa ttcatagatc
     2221 gccgggcgcc gaaccattcc ttcaacggtc acttgcgaac caatcggcgg gatcaaaaca
     2281 ctatcgccgt tttggaggcg ctgaatatcc gatgtcactc catgcagaag caaatcgtac
     2341 aagtccacgt tttgaaccag ttgaccgccg cggaggtgtc gcacaatccg gagagatccc
     2401 tctcgcgtgg gtccgcccgc aataaacaac gcgtttagcg gggtcgagag cgaactcaca
     2461 tcataggcgc ccggatttgc gacgtcaccc accacgtaga tccgaactgt tctaagacgc
     2521 gcaagcgaaa cgtcggccga aatattgcgg aattgagtgc gcaggacctg ctgcaaattc
     2581 tgctgaacct cggcaagact cttcccgctc accagcagcg gtcccacctc gggcaagcta
     2641 atccggcctt cgcgatcgac acttctatat atgcggcgcg acacgcctcc ccacaaatcg
     2701 attgccaagc catctcctgg cccgaccacg taatccggcc ccgccggcag atccatcgga
     2761 atcgcataag ggttctgtga gttgttttga aatacatcca taccaaaccg cgtcgcagct
     2821 gcgggccgag gcgtcgcttg catgtacatg tcataaagcg aaggaatgtc cttatacggg
     2881 ttcggtttgt ggaccattgc ggcaggctgc agaagtgatt ggttcggact cctgaaacgg
     2941 ttggaaaaat agttagggta ataattgctg tatgctgatg ggttcacgcc accgctg
//
LOCUS       DATNDT010000101        21065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1189991, whole genome shotgun sequence.
ACCESSION   DATNDT010000101 DATNDT010000000
VERSION     DATNDT010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 21065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21065
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1189991"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..220
                     /locus_tag="VN788_03015"
     CDS             <1..220
                     /locus_tag="VN788_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19535.1"
                     /translation="SVKKARRADCHFKGGVASNFSRNRAIHSEDVGVANGKDAKFSHR
                     VEDALFAGETLGRNPGDSLGAAIARTAH"
     gene            217..390
                     /locus_tag="VN788_03020"
     CDS             217..390
                     /locus_tag="VN788_03020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19536.1"
                     /translation="MSVEEAAADQTDRVAGFFEKSGDAILHSVQLNQQSAGISGLTAP
                     HLRGKQSHRLYQL"
     gene            complement(460..1686)
                     /locus_tag="VN788_03025"
     CDS             complement(460..1686)
                     /locus_tag="VN788_03025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU19537.1"
                     /translation="MRIAIANWSSRLVGGVEHYISAILETLLDRGHELAFWRQTHGPE
                     NRNVIPAAVRIPIWDSSELGLDRSLAALRRWQPDIIYCQLIPDPATEAKILEVAPSVF
                     FAHDYHGTCISGGKATHFPVLQPCTRRFGPACLGHFYLRRCGGKNPITMVQDYRLNVQ
                     RLRLLTQYNQVITHSIHLAEEYKKHGIPCGRVRFFASGSKLQEGFTAPPWTPRPRWHF
                     LMLGRMLATKGGSLFLNSLPRIASQTSRPIAVTIAGDGPSRARWESKAKRVERQCSNV
                     EIKFPGWLSGIEREDALSDADLLVLPSVWPEPFGMVGLEAGLRGVPAAGFRVGGIPDW
                     LNDGVNGALAPANPPSPEGLADAILRCISTESAYRTLRDAAFGMAKSVNVQLHCDDLV
                     DVFERVLAKSATVSAT"
     gene            complement(1694..2872)
                     /locus_tag="VN788_03030"
     CDS             complement(1694..2872)
                     /locus_tag="VN788_03030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU19538.1"
                     /translation="MDKANFFLAQHLLDRGTPVHLVGHFIDARLVNHPLATIHRIARP
                     LHSYALGAPLLAHSAQIVYRALRRTHPNVRAVANGGNCQLADVNWVHCVHRAWRPRNG
                     EGPWWFQLKRWFTHARACRSEIRAFQRAHLIITNSNTTKHEVERGLGHTNGLIHTVYL
                     GSDVGVTAPTPEEKLRARIDWGISPGRKVAVFVGALGFDGNKGFDTLFKIWRSLCSGP
                     DWDVDLLVAGAGRALADWRAKVHTAGLGDRIRCLGFCDKIPSLLTAADVLISPVRYEA
                     YGLNVQEAICRGVPALTSARAGIAERYPGELKSMLFENPENVPGCMERIRLWRSQPEY
                     WAEKFLRFGSSLRQRDWNIMAAELVAIIEQHCPLTAQSGVVARPAVRDTAIAAMKPSH
                     "
     gene            complement(2946..4076)
                     /locus_tag="VN788_03035"
     CDS             complement(2946..4076)
                     /locus_tag="VN788_03035"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU19539.1"
                     /translation="MRVLQIGKYYPPYMGGIETHLRALCGGLAREMEVEVLVASNNAR
                     EVKERIEGILVTRVATPLHLASAPICPALAARIRASNPELVHVHLPNPAAVLAYLASG
                     HSGPLIVTYHSDTVKQKFLGLLFEPLLHRFLNRASIIIATSPNYLDSSPVLLRHRNRC
                     KVIPYGITVEEFSRTPEPDVSLIRRRFGQRLLIAVGRMVYYKGFEYLVKAMPDVRGHL
                     LLVGDGPLTTSLMDLATSLGVAERVTFLGNLPNEAVIPYYHAADIFVLPSIARSEAFG
                     IVQIEAMAAGKAVVNTDLDSGVPYVSQHGITGITVKPKNSAALAEAINLLLDNGDLRY
                     AYGSAALARVRSEFGLDRMIAQIREIYTEVLSGSCALAVAAR"
     gene            complement(4093..5154)
                     /locus_tag="VN788_03040"
     CDS             complement(4093..5154)
                     /locus_tag="VN788_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002690074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU19540.1"
                     /translation="MKILITGGAGFIGSNAATRYLMAGHKVVVIDNLCREGTQKNLAA
                     LQILGGQLEFMRLDLRDAVAVNAAFEKHRDVDRILHLAAQVAVTTSVLDPRHDFEVNA
                     LGSLNVLEAIRRFGIEAPVIYSSTNKVYGDMKDVGVVLRDNRYAYADFPNGMPESRNL
                     DFHSPYGCSKGVADQYFLDYHRIYGLNTMVFRQSCIYGYRQFGAEDQGWLAWFIIASQ
                     LNHPLTIFGDGRQVRDVLFIDDLLDAYDAGFAAFSSRAGRAYNIGGGAGNVLSLLDLI
                     SYIEKMQGRKIFYSVRNWRAGDQRVFVSDIRRAQNELGWSPRIEYKTGFARLYDWVAS
                     NLHLFEDQPCHLQLAAVSQ"
     gene            complement(5205..6362)
                     /locus_tag="VN788_03045"
     CDS             complement(5205..6362)
                     /locus_tag="VN788_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXU19541.1"
                     /translation="MLHLALVCDFLEERWPSMDLVGDMLFDQFSTRLMDQVNVDRIRP
                     ALRARLSTLPGMRSSKFAWNADRLLNRMADYPRLLGRRLSNYDLYHIVDHSYAQLVHE
                     VPPERTIVSCHDLDTFRCLTQPEIEPRPKWFRAIARRVLDGFQRAAHVICDSEAVRNS
                     ALKSGLLNGDRVTVIHNGIHPSCSSGPDTIADAAANRLLGVPPEALLLLNVGSTIPRK
                     RIDLLLKIFAAVREQVPAARLVRVGGPFTKAQLRLASQLRIADDILELPFLSREVLAA
                     VYRRSTLLLQTSEAEGFGLPLVEAMACGCQVVASDIPVLREIGGDAATFCPVGEVVQW
                     AETVARLLHEKSVGADAWSLRNLASGRNARRFNWEQTANQTLDLYKRISSH"
     gene            complement(6344..7510)
                     /locus_tag="VN788_03050"
     CDS             complement(6344..7510)
                     /locus_tag="VN788_03050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU19542.1"
                     /translation="MWHIVTPEYPPSTGGVGDYTKQIAIGLRDAGDEVHVWCPFWESG
                     DPGHPSWVHPFAGGMKRRNLRRLNEQLDSFPAPRRLLVQWVPHGYGYRSMNLPFCLWL
                     WKRARSGDTVDLMLHEAFLKFGEGNWRQDAAAAVHRLMTLILLRAASRVAVSTPAWES
                     LWKPYALGKRVPFTWLPIPSNIPVTARPEETALLRKRLALQGQPILGHFGTYPRAVTR
                     MLDELLPEIFVAKDGRVLLLMGSASDAYRESLIRKYPEVAGRVLATGTVSSESISSHL
                     AACDLLLQPFPDGITTRRCSAMAALAHGTPMVTTSGYLTESFWEDNRAVAITSAHQID
                     EFIRCVEQLLRDPEELERMGRAARHLYVEKFDTRHVISALRETLVEKEPCYILP"
     gene            complement(7531..8619)
                     /locus_tag="VN788_03055"
     CDS             complement(7531..8619)
                     /locus_tag="VN788_03055"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU19543.1"
                     /translation="MTTASSILHIVPALFDKDDGIVGGAERYAFELARHMAEEEPTTL
                     LSFGAKDMERRVGRLRIRVIGSPLYLRGQKNNPIAAALIREVLKAKVVHCHQQHIIAS
                     SSAALACRLTGRKAFVSDLGGGGWDISSYISTDRWYHGHLHISEYSRKVFGHTSKTWA
                     EVIYGGVDTGIFSPESCASARDTVLFVGRLLPHKGVDYLVRAIPENMRLELIGQPYDE
                     RFVADLHQLAKGKQVIFRHACTDDDLVAAYRKALCVVLPSVYRNCYGDESKIPELLGQ
                     TLIEGMACGVPAVCTNVASMPEVVQDGITGFIVAPNDPDSLREKLIWLKEHAAAAAAM
                     GAAARQRVLDRFTWPGVVKRCLEIYESL"
     gene            complement(8626..9375)
                     /locus_tag="VN788_03060"
     CDS             complement(8626..9375)
                     /locus_tag="VN788_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXU19544.1"
                     /translation="MVYDITGKKITESSFSIMTSVMQNLSECRLRNAKLSGGTSSNEI
                     YAAVACCIEQYHLRGSVLDFGAGRGELTLQLFHMGRFSKIVGVDILERPVNLPETVQW
                     LNADLNDSLSGCGDKTFNTVLMVETIEHLENPRASFREAYRLLQPGGTLILTTPNNES
                     GRSLISLLVRGHYVAFNENCYPAHITALLRKDIERISLETGFLKPVFHYTNFGGIPGM
                     PYISWQSASHRILKGLRFSDNLLAVCQRAAI"
     gene            complement(9321..10472)
                     /locus_tag="VN788_03065"
     CDS             complement(9321..10472)
                     /locus_tag="VN788_03065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012626571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU19545.1"
                     /translation="MLKVLHVGSGNLYGGIETVLATLAKYRNSCPQMEQHFALCFTGR
                     LTLELATAKVPVYHLGKVKASRPWLLANARKRFRKVLENEKFDAVICHSSWPMAMFGK
                     TIFSSKQPLVFWMHGVPSGRHWVDRWARRNRPELMICNSHYTARHGSNLYPGVASCVI
                     RCPVEPSSVPNRSDVRQSIRRELGAPQEDIVIVQVSRMERWKGHLLHLEALATLRDIP
                     GWRCWIVGGAQRPGEANYLREIILAAEKLGIANRVQLLGQREDVRRLLVAADIFCQPN
                     TGVEPFGVVFIEALAGGLPVVTTAVGGPCEIVSEDCGLLTPPGDAPAVARALQRLMID
                     PELRKSMGKAGRERARELCAPETSLHELYDRLWSMTSPVKRLPKVLFQS"
     gene            complement(10525..11472)
                     /locus_tag="VN788_03070"
     CDS             complement(10525..11472)
                     /locus_tag="VN788_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19546.1"
                     /translation="MNLIALGFATAEYFFTVPRFFPRNEVTSIIYASGDVAGYQYYRI
                     PSTFSSAHAFAGAMVVSLPILLGCWTIPNQTSRKRLLYLAGAFSGLLGVLMSATRVHF
                     VIAAVLILIFVLTGNFGNKGRFAIIALLAVLGFFAANNARLGRFESLGNTEYVSERVT
                     GSVNRTFWEVLEEYPIGNGLGGGGTSIPYFLQGLVRRPIAMENEYGRILLELGIVGLL
                     LWVVFIGWFIVGSNAFVQSPWRNGRRLAWACCCGYFVTGMIGLGMLTAIPQSISFLLM
                     IGWVAARPAVENAGMDGCTDSRSVRAIASIRNSHEYALR"
     gene            complement(11880..13130)
                     /locus_tag="VN788_03075"
     CDS             complement(11880..13130)
                     /locus_tag="VN788_03075"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU19547.1"
                     /translation="MRILFLNPTGQIGGAETSLLALVAGLRKLNPSWEFNLITGEEGP
                     LLAKAVALGAKVEVLPFPLSLAGTGDSGLGGAMWERTVERAAMLRGGFASCGYARRLS
                     AAIRRIQPCVIHTNGFKMHLLGSWAAPRKMPIVFHIHDYVSTRALSGRLLRASMKRSA
                     ALVANSNSVAADLAKTIPGAARIVPIYNAVDLGHFTPQGKEMDLDAACDLPPVLPGTI
                     RVGLVATFARWKGHLTFLRALSLLPEHTKIRGYVIGGPIYRTSGSQYSFTEIRDEAAR
                     LGIAQRVGFTGIVEDPAGAIRALDIIVHASTAPEPFGMVIAEAMACGKALIVSNAGGA
                     AEIFEDGITALGHAPGDALTLAGQIGRLSRNGELRTRLGVNARNAAENRFNPDRMADR
                     FSTLYREVAAQPSPLGIACEPALQ"
     gene            complement(13138..15396)
                     /locus_tag="VN788_03080"
     CDS             complement(13138..15396)
                     /locus_tag="VN788_03080"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HXU19548.1"
                     /translation="MHEKSRVKPQPTVVLPPLSADPGPEPAALNYRILVRKYLPVALL
                     LIILGAFAGLAALITDTPMYKARVLLEIQNFSANLSGNSASPILVSDDQGGVNLETQI
                     RLLQSGPVVGEVVDNMLKQPAPAPVRPDVFAKLRHRARRASADPKAVLEQGVMTAADS
                     FDARPVNGTRLVELSCESTHPALAAAFLNNVASRFIQEADRSRTESAEKTNQWLTQRF
                     EDTKVKLQQAEANLRNFVAKSGNIFADPDHTLDDVKLTQLQGDLAKAQTDRIAKQARY
                     QTALNSKPESVPEIAADDTYQTLRSKLEDLRNQRTILSETLTPKHYKIQRLDAQEQEL
                     LASEKDEITAVMNRLRNEYEDAARHEKLLASAYASQGGQVNAQAGNAAQYIALKRDVD
                     NLRQMYDTTLQRINQSGAPGSLPIAPIRLVEPCSPPSAPYKPRPATTIGFGIVAGLAL
                     SVGFAFLRERADRSVKAPGTARILLNIPELGVIPAAGKLPRNANRGLFVGVKRQQPLS
                     ISGQIFSGTPVAEDRSVVHAAWEQGVSMLAESFRTTLASLMREAGTGTSSKAIMITSP
                     QAGDGKTTITSNLGMALAESGRRVVIVDADFRRPRIAKVFGISGSTTLEALIGGTSPV
                     ADYPLDRIAVETSTSGLWILPSYPSETHISRLIYSPRLPLILDRLRREFDVVLLDVPP
                     ILELADARVIGQFVDGVALVIRAGETDRESALAACQCLADDGVPLLGSVLNNLDPRWG
                     KQGSYGYYYNSR"
     gene            complement(15434..16411)
                     /locus_tag="VN788_03085"
     CDS             complement(15434..16411)
                     /locus_tag="VN788_03085"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXU19549.1"
                     /translation="MKKIRILLLVPFLPVAYAQLQQPPTPAPVTEAQFRPAYTLGPGD
                     QVVVRATNVEEFGDRPYRIESDGSMTLPLVGKIHAAGLTVEQFEADLTTRLKEYVRNP
                     QVSVNVAQYRSEVVFFVGAFKAPGIYSLTGRRTLVEMLATVGGLQTNASRRIKITRRK
                     EMGPIPLPNAVQDPSGETTSVQISMNSLEQNVSPAEDLVLRPFDVVSVETAEVIYVTG
                     DVTRVGAVPLGEHDFITMTQLLSLVGGIAPDGSTKVRVLRPVLDSARRAAIDVDVDKI
                     MKGEANDFPLMPNDVLFVPRAGGKAIWSRAAATGVALAIPLVTSLAVTR"
     gene            complement(16408..16740)
                     /locus_tag="VN788_03090"
     CDS             complement(16408..16740)
                     /locus_tag="VN788_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19550.1"
                     /translation="MAVLRPRTRVVFFRVSQEEFQQFMHACESSGARSASDFARMAVQ
                     RMIAAHGAPEPSTQSAREPNNVDATLKHLATRLDQLSELIRERISKQGDSRDPEAAGA
                     LTQWQETR"
     gene            complement(16949..18472)
                     /locus_tag="VN788_03095"
     CDS             complement(16949..18472)
                     /locus_tag="VN788_03095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HXU19551.1"
                     /translation="MGTLAILGVSSFLFALILTPLVRDAFLRWNVVDVPDNRRKLHVT
                     PIPRIGGIGIFSAYLLAFGLLLAIPGPGSTIVGAVLPKVWGMLVAVVIVFATGLIDDL
                     VWLSPKRKLAGQIAAALVAYFSGVEVHLLRGNALDQWTSPLFTVLWLVICTNAFNLID
                     GLDGLASGVGLLATGAIFCSAVAGHNVGLALVTVPLLGCLLGFIRYNFNPASVFLGDC
                     GSLSLGFFLGCAGAIWSEKTTLLGMTAPLMVLSVPLMDTSLSVLRRFLRHKPIFGADR
                     GHIHHRLLARGISVRGTALVLYAVCGVAAVFSVLQHTFYDRVGGILIVLFCVAVAVGI
                     QFLGYIEFGITRRLLTRGYFRQIVDDEVHLKQLEKSLRSAGSMTESWTALSSACKELG
                     FSRVRLKLSGIVRDEVFSHCDETYQLRISLEDGSYVNIYQVLDGDSRPLQVAALARVL
                     RTTMRIPELWAEVPSPTALPALIPLDVTSFNRIVSTSPASLEQSWNHVAESSKTNIA"
     gene            complement(18573..19115)
                     /locus_tag="VN788_03100"
     CDS             complement(18573..19115)
                     /locus_tag="VN788_03100"
                     /inference="COORDINATES: protein motif:HMM:NF033644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UpxY family transcription antiterminator"
                     /protein_id="HXU19552.1"
                     /translation="MSNNSLAWFAVQVRSPLEQWLSVTLREKGYEAYLPTYRSRRAWS
                     DRVKEIDQPLFPRYLFCRFDPMYRLPILKTPNVQGIVSIGRVPIAIPDHEIEAVRRMV
                     SEGKVAGPWPYLREGQHVRIRKGPLTGVEGILTEFKNSYRLVVSIELLQRSIAAEVDG
                     DWVEVLHSASATSRQVARAL"
     gene            19689..20147
                     /locus_tag="VN788_03105"
     CDS             19689..20147
                     /locus_tag="VN788_03105"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HXU19553.1"
                     /translation="MSLKNQTRPIARRDHLLIEYLHEETFVYDAEHHKAHCLNQTVSY
                     VWNNLNGENTVSDLAMLVEREFGVARGEPLVRVALKQLGKACLLEENGAAEENGALPS
                     RREIARKVSMAGGAAFLLPAIASTIAPTPAMAGSWDGGRHRPPPPPPWWW"
     gene            20226..>21065
                     /locus_tag="VN788_03110"
     CDS             20226..>21065
                     /locus_tag="VN788_03110"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXU19554.1"
                     /translation="MPRVTGCRGLRAISGLTAGRAELELLLCCARSAPDAGTIQFIQE
                     LSVSDVNWQRFVELASSHDVLPLVYWNLSRFCADLAPSVLMADLRQKFETNALANLAR
                     AAELRAILQNFHDHGIGVAPFKGPVMAASLYGSPVLRSFGDLDILVRPGDVDRAKDLL
                     FSRGYRIDEQYLPASKHLVFVRADWGSVVELHWAFFDPALRCRLDTRRLWNRVREVQF
                     YGAPAPGLNPEDEILILAVHGANHGWPILKWVCDIAEFVRVYPDLDWRDVLKQAKCAR
                     SERM"
BASE COUNT         4839 a         5920 c         5754 g         4552 t
ORIGIN      
        1 ctccgtgaaa aaagcgcggc gcgccgattg tcacttcaaa ggcggggtcg cgagcaactt
       61 ctcgcgcaat cgcgcgattc actctgagga cgtaggagtg gccaatggca aggatgcgaa
      121 gttttctcat cgcgtcgagg acgccctatt cgcaggagag accctagggc gtaatccagg
      181 ggattcattg ggagccgcga ttgcgaggac agcccattga gcgtcgaaga ggccgccgcc
      241 gaccagaccg accgtgtagc cggctttttt gagaaaagtg gcgacgccat cctgcattcc
      301 gtgcaactca atcagcaaag tgcaggcatc tccggattga ctgctccaca ccttcgagga
      361 aagcaatctc accgccttta tcaactttaa tgatttgggg cccagtgagg agtgggacgc
      421 catttaatgt agcatgcagc aaagtgagca agataattct caggttgcgg aaaccgtggc
      481 tgatttcgcc agaacgcgtt caaacacgtc gaccaaatca tcgcagtgca actgaacatt
      541 cacgctcttt gccatcccga acgccgcgtc gcggagcgtc cggtatgccg attctgtcga
      601 tatacatcgc aaaatggcat cggccaaacc ctcaggagag ggtggattcg cgggagcgag
      661 agcgccattg acgccgtcgt taagccaatc tgggatgcct ccgactcgaa aaccggcggc
      721 cggaacgccc ctaagtcccg cctcgaggcc gaccatcccg aacggctctg gccagacact
      781 cggaagcaca agcagatcag cgtctgacaa ggcgtcctcc ctctcaatac ccgacaacca
      841 gcccggaaat ttgatttcca cattggagca ttgacgctcg actcgtttcg ccttcgattc
      901 ccatcgcgca cggctcggtc cgtcaccggc gatcgttaca gcgatcgggc gtgaagtctg
      961 actcgcgatc cgaggcaaac tattcaaaaa taggctgcct ccttttgtcg cgagcatgcg
     1021 tcccagcatt aggaagtgcc agcgcggccg tggggtccag ggtggagccg tgaatccctc
     1081 ctgtagctta gaaccgctgg caaagaatcg cacgcgcccg cacggaattc catgcttctt
     1141 atactcttcg gccagatgta tgctatgcgt gattacttgg ttgtattgcg tcagtaagcg
     1201 cagccgctgg acgttcaggc ggtagtcctg gaccatcgtg attggatttt ttccaccaca
     1261 gcgccggagg taaaaatggc caaggcatgc cggcccgaac cgcctcgtac acggctgcaa
     1321 aaccggaaaa tgggtagcct tgcctccgct gatgcaggtt ccgtgatagt cgtgggcgaa
     1381 aaacacactg ggagcgacct ctaggatctt cgcttctgtg gcgggatcgg gtattaattg
     1441 acagtagatg atgtccggct gccagcggcg aagagccgca agggatctgt ccagccccag
     1501 ttcgctcgaa tcccagattg gaatgcgaac ggctgccggg ataacattgc ggttttccgg
     1561 gccatgcgtc tggcgccaaa aagctaattc atggcctcga tcgagaagcg tttccaggat
     1621 tgcagatata taatgctcaa ctccccccac cagtcggctg ctccagtttg cgatcgcaat
     1681 gcgcatgacg gagctaatgg gatggcttca tggctgcaat tgccgtatcg cggaccgcgg
     1741 gtcgggccac aacgccggac tgggcggtaa gcggacagtg ctgctcgata attgccacca
     1801 actccgcggc catgatattc caatccctct gacgaagaga ggagccaaat ctcaggaatt
     1861 tctctgccca gtattcgggc tgcgaccgcc atagccgaat gcgctccatg caaccgggaa
     1921 cattctcggg gttttcaaat aacatcgatt tgagttctcc cggataccgc tcggcaatac
     1981 ccgcacgagc agacgtcaac gccgggacgc ctcgacaaat cgcctcctgt acatttaagc
     2041 catacgcctc atatcgaact gggctaatca gtacatctgc cgctgtaagt aagcttggaa
     2101 ttttgtcaca aaaaccgaga catcggattc gatctccgag tccagcagtg tgcaccttgg
     2161 ctctccaatc ggcaagagct cttccagcgc ccgccacgag taagtcgaca tcccaatcag
     2221 gcccgctgca caaggatcgc caaattttga aaagagtatc gaaccctttg ttcccatcaa
     2281 atccgagcgc tccgacgaac acggcgacct tacggcctgg agagattccc caatcgattc
     2341 gggctcgcaa tttttcctct ggagtgggtg ccgtcactcc tacgtcgctt ccaagataga
     2401 cggtatgtat caatccgttt gtatggccga ggccccgttc cacctcgtgc tttgtcgtgt
     2461 tcgaattcgt aatgataagg tgtgcgcgct ggaatgctct tatttcgctc cggcaggctc
     2521 tcgcgtgcgt aaaccagcgc ttcaattgaa accaccaagg gccttctccg ttcctgggcc
     2581 gccatgcccg atgtacacag tgaacccagt tgacgtccgc caactggcag ttgccgccgt
     2641 tcgccactgc ccggacattt ggatgagttc tccttaatgc ccggtatacg atttgcgcgg
     2701 agtgagccaa taatggcgct ccgagcgcat aggaatgcag gggtcgggca atccgatgga
     2761 tggtagcgag cggatggttc actagtcttg cgtcgataaa atggccgacg aggtgtaccg
     2821 gtgtcccgcg gtcgagaagg tgttgcgcca ggaagaaatt tgctttatcc atgccacccc
     2881 gttcatggaa tccgcccgat acgatcaacc acggtgattc aagcttactc gacattcgaa
     2941 gtgatttacc gcgcggcaac agcgagtgcg caagacccgc tcaatacttc ggtatatatt
     3001 tcgcgaatct gggcgatcat tcgatccagt ccgaattcgg aacgaactcg cgcgagggcc
     3061 gccgatccgt atgcatagcg aagatcgcca ttgtccaaaa gcaggttgat tgcttccgct
     3121 agagccgctg aattcttcgg tttgactgta atgcccgtga tgccgtgctg tgagacatag
     3181 ggaactccag aatcgagatc tgtatttacg actgccttgc ctgccgccat tgcctcgatc
     3241 tggacaattc cgaaagcttc acttcgggcg attgacggaa gaacgaatat atcggcggca
     3301 tgataataag gaatgacagc ctcgtttggc aggttgccta ggaacgtgac gcgttccgcg
     3361 acgccgaggc tggttgctaa atccattagc gaggtggtta atgggccatc gccaaccagt
     3421 agaagatggc cccggacatc gggcatagct ttgacaaggt actcaaatcc tttgtaatag
     3481 accatcctgc caacggcgat caataaacgt tgtccaaacc ggcggcggat gagtgaaaca
     3541 tcgggttccg gcgtccgcga gaactcttcc accgttatcc catagggaat gactttacaa
     3601 cgattccgat gacgcagcaa caccggagaa ctatccaagt aatttggcga ggtcgcgata
     3661 atgatgctcg cgcggttgag gaagcggtga agcaacggct caaagagcaa tcctaaaaac
     3721 ttctgcttga ccgtatcgct atgatacgtg acaatcaatg ggccggagtg accgctggca
     3781 agataggcaa gtacggcggc ggggttaggt aaatggacat gtacaagttc gggatttgaa
     3841 gcgcggatac gtgcggcaag agctggacag attggcgccg acgcaagatg cagcggagtg
     3901 gcaacgcgtg tcacgagaat tccttcaata cgttccttta cctctctcgc gttattgctg
     3961 gctacaagaa cctcaacttc catttcacgg gccaggccgc cgcataaagc gcgcaggtga
     4021 gtctcgattc cacccatgta tggaggatag tacttgccaa tctgtaagac tctcattcgt
     4081 tacctatgga tactattgag aaactgccgc aagttggagg tgacagggct ggtcttcaaa
     4141 caagtggaga ttagaagcca cccaatcgta cagacgagcg aacccagttt tatactcgat
     4201 ccggggagac catcccagct cattttgagc gcggcggatg tcgctcacga acacccgctg
     4261 gtctcctgct cgccaattgc ggacgctata gaaaatcttc ctgccttgca tcttctcgat
     4321 ataggagatc aaatcgagta gcgagagaac gttaccagcg cctccaccga tgttgtaggc
     4381 ccggccggcc ctggaggaaa aggcagcgaa accggcatcg taggcatcca gcagatcgtc
     4441 aatgaatagg acatcgcgca cctgccgtcc atcaccgaag atggtaagcg gatgattcaa
     4501 ttgactcgcg atgatgaacc aggcaagcca tccttgatcc tccgctccga attgacggta
     4561 accatagatg caggattgcc ggaataccat tgtgttcagg ccatagatcc ggtgatagtc
     4621 gagaaaatac tggtcagcca ctccctttga gcagccatac ggcgagtgga agtccaggtt
     4681 acggctctcc ggcatgccgt tcgggaaatc ggcgtaagcg taacgattat cgcgaagtac
     4741 gacgccgaca tccttcatgt caccgtagac cttgttagtt gaggaataga taaccggcgc
     4801 ttctattccg aaacgacgta tcgcctcaag gacattcaag cttccgagcg cgttaacctc
     4861 gaaatcatgg cgtggatcga gcacggaagt agttacagca acctgtgcgg caagatgcaa
     4921 gatccgatcc acatcgcgat gcttctcgaa cgcggcattg actgcgaccg catcccgaag
     4981 atccagccgc ataaattcga gctggcctcc aagaatctgc agggcggcga gattcttttg
     5041 agtaccttcc cggcagaggt tatcgataac gactacttta tgacccgcca taagatagcg
     5101 agtcgctgca ttcgagccga taaacccggc tcctccggtg atcaatatct tcatattttt
     5161 gtagcgcttc ccacgctgtg aaggagatac gaacagtacg actgtcagtg gctggatatt
     5221 cgtttgtaaa ggtcgagagt ctgattggcc gtctgttccc agttaaaccg ccgggcattc
     5281 ctgccagaag ccaagttccg aaggctccac gcatctgcac ccaccgattt ttcatgaagc
     5341 agccgcgcga cagtttccgc ccattggaca acttctccca cgggacagaa ggtcgccgca
     5401 tcccctccaa tttcgcgaag aaccgggata tcgcttgcga caacctggca tccgcaggcc
     5461 atcgcctcga cgagaggaag gccaaaccct tcggcctccg acgtctgcaa taaaagcgtg
     5521 gagcgacgat aaacggcggc caggacttcg cggctaagaa agggaagctc caggatgtca
     5581 tcggcgattc tcaattgact ggctagacgg agttgtgctt tggtgaacgg acccccgaca
     5641 cgcaccagcc tggcggctgg gacctgttcg cgaactgccg cgaaaatctt gagtaatagg
     5701 tcgatgcgtt tccgggggat cgtgcttccg acgttcagaa gcaggagagc ctccgggggg
     5761 acccctagca ggcggttggc tgctgcatcg gcgatggtgt caggaccgct gctacaactc
     5821 ggatgaatcc cgttatggat tacagtcacc ctgtcgccat tcaataatcc ggacttgaga
     5881 gcggaattcc tcaccgcttc gctgtcacag attacatggg ctgcccgctg aaagccatcg
     5941 agcactcggc gcgctatggc acgaaaccat ttgggccttg gttcgatctc cggctgtgtc
     6001 aaacagcgaa aggtatccaa gtcgtggcag ctaacgatag tgcgctcagg tgggacttca
     6061 tgaacaagtt gcgcatagct atgatcgacg atgtgataaa gatcgtaatt tgataaccgc
     6121 cggcccagca agcggggata atcggccatg cggttcagta gcctgtcagc attccaggca
     6181 aatttcgaac ttctcatgcc cggcagggtc gatagacgcg cgcgtaatgc cggtcttatt
     6241 cgatcgacat taacctggtc cataagtcgt gtggaaaact gatcaaagag catgtcgccc
     6301 accaagtcca tgctgggcca gcgctcttcc aggaaatcgc agactaaggc aagatgtagc
     6361 atggctcttt ttcgaccagt gtctccctta gcgcggaaat aacatgtcgc gtatcgaact
     6421 tctcgacata gaggtgccgc gcggctctgc ccatgcgctc gagttcttcc ggatcacgca
     6481 ggagctgttc cacgcatcgt atgaattcgt cgatttggtg cgccgaagtt atcgctacgg
     6541 cgcgattgtc ctcccaaaag ctctcagtga ggtatccaga ggtcgttacc atgggtgtgc
     6601 catgggcgag ggcggccatc gcgctgcacc gccgtgtcgt gatcccgtct gggaacggct
     6661 gcagaagcag gtcgcacgcg gccaaatggg aagagatgct ctccgaactc acggtgccag
     6721 tggccagcac gcgacctgcg acttcgggat atttgcgaat cagggattca cggtaagcat
     6781 cactagctga gcccatcagc agcagaacgc ggccatcttt ggccacaaat atctctggaa
     6841 gcaattcatc gagcattcga gtgaccgcac gtgggtacgt gccgaagtga cccaaaatcg
     6901 gttggccttg cagcgcgagc cgtttcctca gcagcgcggt ttcttccggc cgggcggtca
     6961 ccgggatatt gctcggaatg ggcagccacg tgaatggaac gcgtttgccc aaggcataag
     7021 gcttccataa gctctcccat gccggagttg aaactgccac gcgacttgcc gcccgcagca
     7081 gaataagcgt catgagacga tgcacagcgg ctgctgcatc ctgacgccaa tttccttcac
     7141 cgaatttaag aaacgcttcg tgtagcatga gatccaccgt gtcgccgctc cgcgcgcgct
     7201 tccaaagcca caggcagaat ggcagattca tagaacggta gccgtatccg tgggggaccc
     7261 attggaccag cagccgccta ggtgcgggga agctatcgag ctgttcattc aggcggcgaa
     7321 gatttcttcg cttcatgcca ccggcgaacg gatgaaccca ggaaggatga ccgggatctc
     7381 cggattccca aaagggacac cagacgtgaa cttcatctcc cgcatcgcgt aagcctatgg
     7441 caatctgctt cgtataatca ccgacaccgc ccgtgctggg tggatactcg ggagttacta
     7501 tatgccacat ctttgaccta ggagaaccaa ttacagagac tcgtatatct caagacagcg
     7561 cttaactacg ccgggccatg taaagcgatc aagaactcgt tgacgggcag ccgcacccat
     7621 tgcagcggca gcggctgcgt gttccttgag ccatatgagc ttttctcgca ggctatcggg
     7681 atcgttaggg gccacaatga atccggtaat tccgtcctga acgacctcgg gcatgctagc
     7741 cacattcgtg cagacggcgg gaacgccgca cgccattccc tcaatcagcg tctggccaag
     7801 cagctcaggg atcttgcttt cgtccccgta gcagttccga taaacacttg gcaacaccac
     7861 gcacagcgct ttccgatagg cggcgaccag atcgtcatcc gtgcaggcat ggcgaaaaat
     7921 tacctgcttt cccttcgcaa gctggtgcaa atcagccacg aaccgttcat cataaggttg
     7981 gccaatcaat tccaatcgca tgttttccgg gatcgccctt actagataat caaccccttt
     8041 atgaggaagt aatcgcccaa cgaaaagcac ggtatcgcgc gcgcttgcgc atgactccgg
     8101 cgaaaatatt ccggtatcta ctcccccgta tatcacttcc gcccaagtct tactggtgtg
     8161 tccaaaaacc ttacggctgt actcgctgat atgcagatgc ccatgatacc aacggtcggt
     8221 cgaaatgtaa gaagagatgt cccaaccacc accgccgaga tcgctgacga aggctttccg
     8281 tcccgtgagt ctgcaggcta gtgcggccga ggaactggcg ataatgtgtt gctgatggca
     8341 atggactact ttagccttga gcacttcccg aatcaaagca gccgcaatcg gattgttctt
     8401 ttggccacgc aaatacaaag gagagccaat aacccggatc cgaaggcggc cgactctccg
     8461 ctccatgtcc ttcgcgccaa aactcaacaa cgttgtgggt tcttcttccg ccatatgccg
     8521 tgccagttca aatgcgtatc gctcagcccc gccgacgatt ccgtcatctt tgtcgaatag
     8581 tgctggaacg atgtgaagaa tagatgaagc cgtcgtcata gtaggctaaa tcgccgcccg
     8641 ttgacaaact gccaataaat tatcgctgaa acgcaaacct ttcaatatcc ggtgagacgc
     8701 tgattgccag cttatatagg gcataccggg aatgcctcca aaattggtgt agtggaatac
     8761 cggcttcaaa aaccccgttt caagcgatat acgttcaata tccttacgga gcagggccgt
     8821 gatgtgtgct gggtaacagt tttcattgaa cgcaacatag tgcccacgga ccagcaggga
     8881 gataagtgac cgaccgcttt cattattggg ggttgtcaga atgagcgtgc cgccaggttg
     8941 aagaagccga taagcctcac gaaacgacgc tcgaggattt tctaaatgct ctattgtttc
     9001 caccattagt accgtgttga acgttttatc tccacacccg ctcaaactgt cattcaggtc
     9061 cgcgttaagc cactgaacag tctccggaag attcacgggc cgttccaaaa tatccacacc
     9121 gacgattttt gagaatcgac ccatgtgaaa cagttgcagc gtcagttcac ctcttccggc
     9181 tccgaagtcg agtaccgatc ctcgaaggtg atactgctct atgcagcacg cgaccgctgc
     9241 atagatttcg ttactgctgg tgccgccgct gagtttcgcg ttgcgaagac ggcactctga
     9301 gaggttttgc ataaccgagg tcatgattga aaaagaactt tcggtaatct ttttaccggt
     9361 gatgtcatag accacaagcg atcgtaaagc tcatggagag aggtttcagg tgcgcagagt
     9421 tctcgcgccc gctcccggcc ggctttcccc atggattttc gtaattctgg gtcgatcatc
     9481 aatcgctgta gtgcgcgcgc caccgccgga gcgtcaccag gtggcgtcaa taggccacaa
     9541 tcctcggata cgatctcgca tggaccgccc acggcggtgg tcaccaccgg aagaccacct
     9601 gccagagctt cgatgaagac taccccaaac ggctccacac cggtattagg ctgacagaaa
     9661 atgtcagcgg ctaccaacag tcgcctgaca tcttcgcgct gtcccaacaa ctgcacgcga
     9721 ttcgcaatcc caagtttttc cgcggccaag atgatttccc gcaagtagtt cgcttctccc
     9781 ggccgctgcg ctccgcccac gatccaacat ctccagcccg ggatgtcgcg tagagttgcg
     9841 agtgcctcca gatgcaacaa atgccctttc caccgctcca tccgactgac ctgaacgatc
     9901 acgatatctt cctgcggcgc acccaactcc cggcgaatcg actggcggac gtctgaacga
     9961 ttcggcacac tgcttggctc gaccggacag cggatcacgc aactcgctac tccaggatat
    10021 aaatttgaac cgtgtcgagc tgtgtagtgg ctgttgcaga tcatcagctc cgggcgattt
    10081 cgtcgagccc atcggtctac ccagtgccgg ccgcttggca cgccgtgcat ccagaatacg
    10141 agtggctgtt ttgaggaaaa tattgttttg ccaaacatgg ccataggcca agatgaatgg
    10201 cagatcacgg cgtcaaattt ctcgttttcg aggaccttgc gaaagcgttt cctcgcgttc
    10261 gcaagcaacc acggccgact ggctttgact ttccccaaat ggtaaacagg aactttcgct
    10321 gtagctaatt ccaaggtgag gcgtcccgta aaacacagag cgaagtgttg ctccatttgt
    10381 ggacatgagt tccggtattt tgccagcgtg gccaagacgg tttcaatgcc gccgtataaa
    10441 ttaccgctgc cgacatgcag cactttcaac attctttttg gatagtcctc cgcaggcatg
    10501 acaatacttc gatccaggac gctattatcg aagagcatat tcgtgcgagt tgcgaattga
    10561 agcgatcgca cgaacgcttc gtgaatcggt gcagccatcc atacccgcat tctctaccgc
    10621 cggccgtgcc gcgacccagc cgatcattag aagaaaggag atgctttgcg gaattgccgt
    10681 caacatacca agcccgatca tgccggttac aaaataaccg cagcaacaag cccacgcgag
    10741 ccgccggcca ttccgccaag ggctttggac gaaagcgtta gatccgacaa tgaaccaccc
    10801 aatgaaaaca acccataata ataatccgac gattccaagc tctagcagaa ttcgcccata
    10861 ctcgttctcc atggcgatcg gcctgcgcac gagaccttgc aggaaatagg ggatgctcgt
    10921 cccacctccg cccagaccgt tgccaattgg atactcttcg agaacctccc agaaagtacg
    10981 attcacgctg ccagtaacac gctctgaaac atattcggta ttgcccaacg actcgaaccg
    11041 gcctaagcgg gcattatttg ccgcgaaaaa tcccagaaca gcgagcagtg caatgatggc
    11101 gaaacgtcct ttatttccaa aattgccggt taaaacgaaa attaagatca acacggccgc
    11161 aatcacgaaa tgcactcggg tcgcggacat gagtacgccg agcaagccgg agaaagcccc
    11221 cgcaaggtat agaagtcgct ttctggaggt ttggtttgga atcgtccagc atcccaataa
    11281 aattggaagc gacactacca tcgctccggc aaaggcatgc gcgctggaaa acgtgctcgg
    11341 tattcgatag tattggtatc ctgcgacgtc gccgctggca tagatgatgc tggttacctc
    11401 attgcgcggg aagaatcttg gcacggtgaa gaagtactcg gcagtggcaa atcccagcgc
    11461 aatcaagttc atcgtggcaa ttcctttgcc caatctaagg agatcgctcg tgtctagcat
    11521 tactccaacc aggatcattg gaagaaagaa gatattcccg cgcaaaccaa cgagggaaat
    11581 aaggggattt tgcaggggca agaggcaaac aaagcagggc catgcgatca aaacaatgac
    11641 ccatgttttt acggtccgca gtcgggggga cgtgttcgaa aatagttttc cggcgtgagt
    11701 gaaatagaag ccgatcagac tcgaatcgaa tataaagtgc gatgccgccg taggaaagtt
    11761 agcgcgaagg atcccataga aatagcccca agcgagtacg gcaattaagc cctttgccat
    11821 cgatttccga ctggcccaaa ggcttgtggc gaaggcgatg acgcataaca gaagcgcgat
    11881 cattggaggg cgggttcaca ggcgattcct agcggcgatg gttgcgccgc aacctcccta
    11941 tatagggtcg aaaaccggtc cgccattctg tcggggttga aacgattctc cgcggcgttt
    12001 cgggcattta ctccaagacg ggtgcgtaat tccccgttcc tgctcaatcg cccgatttgt
    12061 ccggccaacg tcagcgcgtc tccgggcgcg tggccgagcg cggtaattcc atcctcgaaa
    12121 atctccgcgg cgccccctgc gttgctcacg atcagcgcct ttccgcaagc catcgcttcc
    12181 gcgatcacca tcccgaacgg ctcgggggcg gtactcgcgt ggacaatgat atccagcgct
    12241 cttatcgctc ctgccggatc ttcaacaatg cccgtgaagc ccacccgttg cgcaattccg
    12301 aggcgtgctg cctcgtcccg gatttcggta aacgagtact ggcttccact ggttcgatag
    12361 atcggtccac cgatgacgta accgcggatt ttagtgtgtt caggaagtaa cgaaagcgcg
    12421 cgaagaaaag tcaggtggcc cttccatcgc gcgaacgtcg cgaccaatcc cacgcgaatg
    12481 gttccgggca gcactggagg aagatcgcag gccgcatcga ggtccatctc ttttccttgc
    12541 ggcgtaaagt ggccgagatc gaccgcgtta taaattggaa cgatccgcgc cgcgccaggg
    12601 attgttttcg cgagatcggc tgccacgctg ttcgaattcg cgaccagcgc cgcgctccgc
    12661 ttcatgctgg cgcgaagaag ccggccagat aacgcacgcg tgctcacgta atcgtgaatg
    12721 tgaaaaacaa tcggcatttt gcgcggagcg gcccacgagc cgagcagatg catcttgaaa
    12781 ccgttggtgt ggatcacaca gggctggata cggcggatcg cggcggatag gcgcctcgcg
    12841 taaccacaac tcgcgaagcc accccgcagc attgcggccc gttccacagt gcgttcccac
    12901 atcgcgccgc ccaatcccga atcgcctgtg ccggcgagac taagcgggaa cggaagcacc
    12961 tcgaccttag cgcccaaagc aaccgctttg gccaagagtg gtccttcttc tcccgtaatc
    13021 agattgaatt cccaggaggg gttcagcttt cgaagtccgg ccacgagtgc gagcaggctt
    13081 gtctccgcgc cgccgatttg ccccgtggga ttcaggaaaa gaattctcat gactctgcta
    13141 gcgggagttg tagtaatatc cgtagcttcc ctgcttgccc cagcggggat cgaggttgtt
    13201 aagcacactg ccgagtagcg gtactccatc atcggcaaga cactggcaag cggcgagcgc
    13261 gctttcccgg tcagtttccc cggcacggat cactagggcc actccatcta cgaactgtcc
    13321 gattacgcgc gcatcggcta gttccagaat tggaggaacg tccaacagga caacatcgaa
    13381 ttcccgccgc agccgatcga ggatcaacgg aagtctcggc gaatagatta gccgggaaat
    13441 gtgagtctcg gacggatagc tcgggagaat ccagagtccc gaagttgacg tctcgacggc
    13501 aattcgatca agcggatagt ccgcaaccgg ggaggttcct ccgatcaacg cttcgagtgt
    13561 cgtgcttccg gatatcccaa aaactttggc gatccgcggc cgccggaaat ccgcatcgac
    13621 tattactaca cgccggccgg actcagccag cgccatacca agattcgagg tgatcgtggt
    13681 cttgccgtcg ccagcttgcg ggctggtgat catgatcgct ttcgatgagg ttccggtgcc
    13741 cgcctcacgc atcagggaag caagcgtcgt gcggaacgac tcggccagca tggagacgcc
    13801 ttgttcccag gcggcatgta cgacgctgcg atcctcggcc acgggcgtgc cagaaaatat
    13861 ctgtccggag atggatagcg gttgctgcct tttcacgccc acgaataagc cgcgattcgc
    13921 attccgcggc agcttgccgg ccgccggaat cacgcccagt tccggaatat tcagcaagat
    13981 gcgcgccgtg ccgggcgctt ttacgctgcg atccgctctt tcccgcagga aagcgaatcc
    14041 gacggataag gcgagtccgg caacaattcc aaacccgatc gtagtagccg gccttggttt
    14101 gtatggtgcg gaaggcggag aacagggttc taccaggcgg atcggggcga tgggcaatga
    14161 gccgggagcg ccgctctggt taatccgctg cagggttgta tcgtacatct gccgcagatt
    14221 gtccacatcc cgcttcagcg cgatatattg agccgcattg ccagcctgcg cattaacctg
    14281 accgccttgc gaagcataag ccgacgcgag cagcttctcg tggcgggccg catcttcata
    14341 ctcgttccgt agacggttca tgaccgctgt gatctcgtcc ttctcagacg caagcagttc
    14401 ctgctcctga gcgtcaagtc gttgaatttt gtagtgtttc ggagtcaacg tctcgctcaa
    14461 gatggtcctt tggttacgca aatcctcaag tttcgagcgc agcgtctggt acgtatcgtc
    14521 cgccgcgatt tccggtacgg actccggttt actattaaga gccgtctggt aacgggcttg
    14581 tttggcgatc cggtcggtct gcgctttcgc cagatcgcct tgtaattgcg ttagtttcac
    14641 gtcgtccagc gtgtggtcgg gatcggcaaa gatgtttcca gatttcgcca cgaagttgcg
    14701 caaattggct tccgcctgct ggagtttgac cttagtgtcc tcgaaccgtt gcgtgagcca
    14761 ctgatttgtc ttttcggcgc tttctgtgcg agatcggtcg gcttcctgga taaaccgact
    14821 cgcgacattg ttcaggaacg cggctgccaa tgccgggtgc gtggattcgc aactcagctc
    14881 cacaagccgc gttccattga ccgggcgggc gtcaaaactg tcagccgccg tcatgactcc
    14941 ctgttcaaga accgccttgg gatccgccga tgcgcgccgg gcacgatggc ggagtttcgc
    15001 gaaaacatcc ggcctgacgg gggcgggcgc cggttgtttg agcatattgt cgacgacctc
    15061 gccgacgacc gggccggatt gcagcagcct gatctgggtc tcgagattga cgccgccctg
    15121 gtcgtcgctt accagaattg ggctggcgct gtttcccgag agattcgccg aaaagttctg
    15181 aatctccagc agcacacgcg ccttatacat cggagtatcc gtaatcagag ccgcaaggcc
    15241 agcgaatgcg ccgagaatta tcagcagcag cgcgaccggc aaatattttc ggaccaggat
    15301 tcgataattg agcgcagccg gttcgggccc cggatcggcg gataaaggtg ggagtaccac
    15361 ggtaggctgc ggcttcaccc gcgatttttc gtgcatttcg tgtttctccc gatcaggcat
    15421 gtcttcgacc ggcttagcgc gtaaccgcga gactagtgac aagaggaatg gccagagcga
    15481 ctcccgtagc cgccgcgcgc gaccagatcg ccttccctcc cgcgcgaggg acgaagagta
    15541 catcgttggg catcagcggg aaatcgtttg cctcgccctt cataatcttg tcgacatcta
    15601 catcgatcgc cgcgcgtctg gccgaatcga gcacggggcg caagacgcgg actttcgtcg
    15661 agccatccgg ggcgatgccg ccgacgagag acaaaagctg agtcatggtg atgaagtcat
    15721 gttccccgag cggcactgca ccgacgcgag ttacatcgcc ggtcacataa atcacctccg
    15781 ctgtctcgac cgacaccacg tcgaacggcc ggagaacgag atcctcggcc gggctcacgt
    15841 tttgttcgag actgttcata ctgatctgca ctgaggttgt ctcgcccgat ggatcctgaa
    15901 ccgcattggg cagcggaatc ggccccatct ccttgcgacg cgttattttg atacggcggc
    15961 tggcattagt ctgcaatccg ccgacggtcg ccagcatctc taccaacgtc cggcgtccgg
    16021 tcagtgaata gatgcccggc gctttgaatg ctccgacgaa gaacaccact tccgagcggt
    16081 actgtgccac gttgacactg acctgcgggt ttctcacata ctctttcagt cgcgtcgtga
    16141 gatccgcctc gaactgctcg accgtcagac ccgccgcgtg aattttccca accagcggca
    16201 gagtcatgga gccgtcgctc tcgatccggt acggcctatc gccgaactct tccacgttcg
    16261 tggcccggac caccacctga tcgccaggac caagcgtgta tgcgggacgg aactgcgctt
    16321 cggtaaccgg cgctggagtt ggcggctgtt gaagctgcgc gtatgccacg ggcagaaatg
    16381 gcacgagcag tagaatacgg atcttcttca tcgtgtctcc tgccattgcg ttagcgcacc
    16441 cgcagcctcg ggatcccggg aatctccttg cttgctgatg cgctcccgta tgagttcgct
    16501 gagttgatcg agacgtgtgg ccagatgctt cagtgtcgcg tcaacgttgt tcggttcccg
    16561 agccgattgc gtcgacggct cgggagcgcc atgagcggcg atcatccgct gcactgccat
    16621 gcgggcgaag tcggaagcgc tccgcgctcc cgagctttca cacgcatgca tgaactgttg
    16681 aaactcctct tgcgaaaccc ggaagaatac tactcgggtg cgcggcctta gcacagccat
    16741 tgcctgcccc ctaatacggt ctgcatgata attgcctcac gatgtgattg taaatatagg
    16801 aaactctaaa tatgtggatc gaacggacaa gctaccgctt attcgatccc atctaaaatt
    16861 tgcaccttgt gggttcttag aaagagaagt tactgtatac tcatcttgag tctcttattt
    16921 acaaaagtca atagtactga ctcacttact aagcgatgtt agttttggat gattccgcca
    16981 catggttcca ggattgttcg agggaggcgg gagatgtcga aacgattcgg ttgaagcttg
    17041 ttacatcgag cggaattagc gcaggaaggg ccgtcgggga cggaacttct gcccatagct
    17101 ctggaattcg catggtagtc cgcaaaactc gcgcgagcgc agccacctgt aagggcctcg
    17161 agtccccatc gagcacctgg taaatgttga cgtaactacc atcctcaagt gaaattcgaa
    17221 gctggtaggt ttcgtcacag tggctgaaca cttcatctcg gactataccg gatagcttga
    17281 ggcgtaccct cgagaaacct aattctttgc aggctgaaga gagggccgtc caggactccg
    17341 tcatcgagcc ggctgagcgc agcgactttt ctagctgctt taagtggacc tcgtcgtcga
    17401 cgatttgccg gaaataaccg cgtgttagca gccggcgtgt aatcccaaat tcgatatacc
    17461 ccaagaattg aatacccacc gctaccgcaa cgcagaaaag aacaataagg atgccgccga
    17521 cgcggtcgta aaaagtatgt tgcaataccg aaaacacagc ggctacgccg cagacagcgt
    17581 agaggaccag ggcggttccg cggacggata tgccgcgtgc gagcagacgg tggtgaatgt
    17641 gcccgcggtc cgccccgaag atcggcttgt ggcggagaaa acggcgtaat acggaaagcg
    17701 aagtgtccat cagggggacg gataagacca tcagcggagc ggtcattccc agaagtgtcg
    17761 ttttttcact ccaaatggcg ccggcgcatc ccaggaaaaa tccgagcgat aaactgccgc
    17821 agtcgccgag aaacaccgaa gctggattga agttgtatcg aatgaatccg agcaggcagc
    17881 cgaggagggg cacggttacg agagccagac caacattatg gcccgcaacc gccgagcaga
    17941 aaatcgcccc tgtcgcgagc agtcccacgc ccgaagcgag gccatccagt ccatcaatga
    18001 gattgaaagc gttcgtgcag atcaccagcc ataaaaccgt gaacagcgga ctggtccatt
    18061 gatccagggc attcccgcgc aggaggtgga cctcaacacc ggaaaaataa gccacgagcg
    18121 cagccgcgat ttgtcccgcc agtttgcgtt tcggtgagag ccagaccaaa tcatcgatca
    18181 gccccgtggc aaatacaatc acgaccgcca ccagcatgcc ccagaccttc ggcaacaccg
    18241 ctccgacaat cgtgctacct ggtcccggga tcgccagcag caagccaaaa gccaataagt
    18301 aggcactgaa gataccaatt ccgccgatcc ggggaatggg agttacatga agtttccgcc
    18361 ggttatctgg tacgtcgacc acgttccagc gcaggaaggc gtctcggacc agcggcgtga
    18421 gtatgagagc aaaaagaaat gaactgaccc caagaatggc taacgtgccc atggtgatct
    18481 ctactagcga atcaagatat aaaacgcccg aggcgtgaaa cggatattaa gcgtggaaag
    18541 caagcggaaa gaactatcgc gtgtcggcga acttaaagag cgcgggccac ctggcgagat
    18601 gtggctgagg cggagtggag aacttccacc caatcgccgt cgacttccgc tgcgatggac
    18661 ctttgcagca actcaatgga gacgactaga cgatagctgt tcttgaattc cgtcaggatt
    18721 ccctccactc ccgtgagcgg cccttttctg atccggacat gctgcccttc gcgcaaatat
    18781 ggccacggtc cagccacttt gccttctgaa accatcctcc ggactgcttc aatttcgtga
    18841 tccgggatgg cgatcgggac tctaccgatg ctgacgatgc cttgcacgtt gggcgttttg
    18901 aggatcggga ggcggtacat cggatcgaat cggcaaaata agtatctcgg gaacaggggc
    18961 tgatcgattt ctttgacccg gtcagaccaa gcccgcctcg aacggtaagt cggaagatac
    19021 gcttcgtaac ccttctcgcg cagggtaacg gagagccact gttccagagg cgaccgcact
    19081 tgaactgcaa accaagccaa agaattattg ctcataactt actccaaaca ctcctaaaag
    19141 atgtccgtga ttttgccggc cgaggacaaa gctaccgctt cactagtcac atccggctag
    19201 tcacgattcg cgctttcgcg cgctatttac tgggtgtact cacatattca ggtacctctt
    19261 aactcttgtc aatgcacttt agtccctcct caagcaggca cgatttgcaa atcgttccgg
    19321 tacgttcagt ctcgatgcag tccgtagcga ctacttacgt aaggcgcatc actggaaacg
    19381 tttctagcgg cggtaaatta atgtgaaatg gtaaataatt ccgtgcaatt gtagcggaaa
    19441 caaccttttg gtaccgcagt gtccgtacta ggagggtttc agtagtatgc agtacgtgga
    19501 tgattacttg gagtgtattt tagcttgatg gcgggtaacc gctcgcctat actaacatcg
    19561 tcggaacagg taagcgtagt gggcaatggc cttgagtggc gcgccattga gagtgttgct
    19621 aatgagcggc attatctgat tttgttgtga gacatagctg aggcaaacgg taagttgcgg
    19681 gagatggagt gagtttaaaa aatcaaacga gaccgattgc acgcagagat catcttctga
    19741 tcgagtatct acatgaagag acatttgtct acgatgcgga gcaccacaaa gcgcattgct
    19801 tgaatcagac tgtctcctac gtttggaaca acctgaatgg agagaacacc gtgtcggacc
    19861 ttgccatgtt ggtcgaacgc gagtttggcg tcgctcgcgg tgagccgctg gttcgggtgg
    19921 ccctaaagca attgggaaag gcctgcttac tcgaagaaaa cggtgcggct gaagaaaacg
    19981 gcgcgttacc atccaggcgg gagatagcgc gtaaggtgtc gatggccggt ggcgcggctt
    20041 tccttctgcc agccattgct tcaacgatcg cgccaacgcc cgccatggcg ggatcctggg
    20101 atgggggacg ccatcggccg ccgccgcccc ctccttggtg gtggtgatgg aattaaatcc
    20161 tagcttgctg ggacgcaaca gattctgcga ccgctctcgc ggtcgtggct tggaataagt
    20221 gtttcgtgcc gcgcgttact gggtgtaggg gtctgcgcgc gatttcgggt ttgacggctg
    20281 gtcgcgcaga gttggagctt ttgttgtgct gtgcccggag cgcgcctgac gcgggtacta
    20341 tccagttcat tcaggaatta tcagtttcag atgtcaattg gcagcgtttc gtcgaactgg
    20401 cttcatcgca cgatgttctc ccactggtct attggaacct gagccggttc tgcgcagact
    20461 tggctccgag cgttctgatg gcggatctcc ggcagaagtt tgaaacgaat gcactagcga
    20521 accttgcccg agcagcagaa cttcgggcga tccttcaaaa cttccacgat catggcatag
    20581 gagtggcgcc tttcaaaggc cctgtaatgg cagcctctct gtatggatct ccggtgttgc
    20641 gctccttcgg cgatcttgac attctagttc gccccggcga tgtggatcgc gcgaaggatc
    20701 tcttgttctc acgaggctat cggatcgatg aacagtatct ccctgcttcg aaacatctcg
    20761 tctttgtccg ggcggactgg ggttccgttg tcgaactgca ttgggcgttt ttcgacccgg
    20821 ctttgcggtg ccgcctggat acccgccgct tgtggaaccg cgttcgtgaa gttcagttct
    20881 acggagcacc ggcaccggga ctaaatcccg aagacgagat cctgatcctg gcggtccacg
    20941 gagcaaacca cgggtggccg attcttaaat gggtttgcga tattgccgaa ttcgtgcggg
    21001 tttatccgga tctcgattgg cgagacgtgc tgaagcaagc caaatgcgcc cgttcggagc
    21061 gaatg
//
LOCUS       DATNDT010000102        11650 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2079005, whole genome shotgun sequence.
ACCESSION   DATNDT010000102 DATNDT010000000
VERSION     DATNDT010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11650
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2079005"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            567..875
                     /gene="flgM"
                     /locus_tag="VN788_03115"
     CDS             567..875
                     /gene="flgM"
                     /locus_tag="VN788_03115"
                     /inference="COORDINATES: protein motif:HMM:TIGR03824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HXU19555.1"
                     /translation="MKINSEFEPQAGQGPSATGAAGGASHVHSSLNSHAGRSSETDKA
                     DLSSEAQQFAKLSAHANKVPDVRADRVATLKNAIQNGSHSVSNHQIAQAMARDSHPRA
                     "
     gene            1033..1212
                     /locus_tag="VN788_03120"
     CDS             1033..1212
                     /locus_tag="VN788_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19556.1"
                     /translation="MFDQTSKIAAINSFREPETGTSEHGQKRKPQGKHFAQAVPVVES
                     SGSDDGDEHELDTLA"
     gene            1209..1703
                     /locus_tag="VN788_03125"
     CDS             1209..1703
                     /locus_tag="VN788_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19557.1"
                     /translation="MNSSIESAALLGALGKQLRLFRELSADLVASRTAYGTMNLAAIY
                     QHIATQTAICEEFRQIEDDRKSAWRAACTAVDLDAEKSDLRELTARMDRSFAEPIRDV
                     VTKLAIAEGELRNLNRAHTVLIEGSRRTLAILGNVLASFAPTYGRPAGISEPAAAAPK
                     GAVR"
     gene            1700..3076
                     /gene="flgK"
                     /locus_tag="VN788_03130"
     CDS             1700..3076
                     /gene="flgK"
                     /locus_tag="VN788_03130"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HXU19558.1"
                     /translation="MSLGSSLSIAVQAMLTDQGALAITSNNISNVNTPGYTRQVANLE
                     EAPPAQYGNLNFGGGVALANIQSIRNNVLQLRLNQETQAQAKLNTFTNGMDQIQTVFN
                     EASGTGLQSLLSNFFNSFQSLSVDPANDGDRQAVIGTAQSLAAGFQQSATTLVQQQQN
                     SDQGVVQTVQQINQLTAQIATLNGEITATAGAGLNTNSFEDQRAQLITQLSGLIDVRT
                     ISANGNTVTLTTNNGAELVVGNQSFDLQTGVNASTSFHDVYAEGTDISSSIQGGSLAG
                     DIHLRDQEIPSLQNNLDTLAYSIENAVNTQNAAGFDLNGNPGGNIFQPPTGVSGAALN
                     MTVMLTDPNLIAASGDGTPGDNANAIALADLQKQNIVSGQPPVAYYAALVSQIGNDAA
                     TASSQLTGGTLLIQQLNDQINSVSGVSLNEEGANLIQYQNAYAAASRVATVVASLYQT
                     VINMVPAS"
     gene            3258..4154
                     /gene="flgL"
                     /locus_tag="VN788_03135"
     CDS             3258..4154
                     /gene="flgL"
                     /locus_tag="VN788_03135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HXU19559.1"
                     /translation="MSIRVNPNIIPDMIAGLSQTQQQLNQADQQIASGRTIDQPSDNP
                     AGMAALILNHDVQSQTDTFSTNVSNLQDRLQTADSALSSAITAVNQAISLGVEAGNST
                     LSNIDRQAIVSQLSGIQQQLVSLANTAYGGTYLFGGSLVETTPFALDSISPNGVTYSG
                     NSSTLSVEINDGATVATNVPGDQVFLNPSGSLMGAIQGLITAIQNNSGISAASVTLGT
                     AASVFNGERVTYGSSLTQLQSMGNFLASQQTQLATQENNIDAADLAKASSNFSQASVA
                     YQSLIEAEANILKLPNLLTFLQ"
     gene            5950..6513
                     /locus_tag="VN788_03140"
     CDS             5950..6513
                     /locus_tag="VN788_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19560.1"
                     /translation="MFSGVLGKRAEPCFLGPPFKEPPRCAQFERFGRRRRRWPQVVNV
                     NNSALDRELRETLDSRSQIEFALYLIKERTSMSIKRFIAQEQEINCGTAATQSVSTSW
                     FGRLSAEIVHRCRLALFAPTVQRSLAVERERRQIEREQMAEEYARGYLEGWHQCYSAC
                     LEAVEESVAENRDIWTAGEVLVGSRAN"
     gene            6582..6971
                     /locus_tag="VN788_03145"
     CDS             6582..6971
                     /locus_tag="VN788_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19561.1"
                     /translation="MRSKEVFEVCHGKYKGLRNWLSDSTGAVKSVDLDLPLVGYQWRP
                     NRGKACEYVADFERIGRHGLRRPEWKGRLRLFETYFVTGMDYQKARSAVGVAEGTFDY
                     WYQEVKKTLGAEFSRTGLYPPSKYFHA"
     gene            7311..7526
                     /locus_tag="VN788_03150"
     CDS             7311..7526
                     /locus_tag="VN788_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19562.1"
                     /translation="MQILTEGQTVRHEQYGIGTVTESNNERTTIDFDNHGLKKFVTSI
                     WTAELIGEPPADRAPRRRGRRKAPKKA"
     gene            complement(7559..8239)
                     /locus_tag="VN788_03155"
     CDS             complement(7559..8239)
                     /locus_tag="VN788_03155"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU19563.1"
                     /translation="MTAIVKNYKIVVADDHAVVRRGLRALLDAQPGLEVCAEASSGPE
                     TLDCVRKFRPDLVVLDLTMPDMNGLDVTRNIRATSPSTAVVVLTMHFSEELAREVLRA
                     GALAYVLKSDADGELLAAIDHVRHKQPFFTGALALTMAHSFLHGPAKPQEAPGRKPLR
                     GSPLTRREVDVLQLLAEGQSNKQAAMELGVSIRTIESHRTHIMHKMGFQNFSELVRFA
                     VRNNLVDL"
     gene            complement(8247..9776)
                     /locus_tag="VN788_03160"
     CDS             complement(8247..9776)
                     /locus_tag="VN788_03160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXU19564.1"
                     /translation="MPGQSPDSLINIFWTDALSASSSHSINNDLIGIYTWHWHIKSNT
                     FVWCIDQFKLSSRRRYSCADGIDTFLQLVSAQDRDIVAENVKAAMSGISCACDITLHQ
                     ENGGDRHLLLRILPICARSGQVVSVVGFCCAKSSASQEIPSQRAVLLTQAEEIARFGT
                     WEFDIESRIATLSPNLAQMLGIAPEICLTEDRYWKRIHSADRTSVLESVRSAIAAGTP
                     FQYVARYLSPMGGTRHHFVRGLPNFEVTGGLKSIVGITFDFSDQNQIEGELRRLSQKL
                     LRTRDDERRAIARELHESTGQTLAAVKMSLGRLRDVLPESDDQVNKFLESAVQLAETA
                     IREIRTLSYLMHPPMLDEAGLRSAVRWYAKGFTERSGIPIEVFIPEDFGRHRQEIETT
                     LFRIVQEALTNIHRYSGSRTASIRLTVEDGLVHAVIQDDGCGLSNPGTTHGRDFPLGV
                     GILGMRERVQQLNGTFEIYTAAGKGTTIRTTLPADSSSKTVREKKPIRTRHKQASSRI
                     G"
     gene            complement(10025..10864)
                     /locus_tag="VN788_03165"
     CDS             complement(10025..10864)
                     /locus_tag="VN788_03165"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXU19565.1"
                     /translation="MIPFLHLGPITIPTYGLMVATAMIFAYFVLRADFARRGIAKGSE
                     SNLAELFIAVPCICGIIGARLYHVLETPRELLADPWGQLFSRYGLAWFGGLIAGAAAW
                     IFLSRRHRIPLLVSLDAGSPAAALGYGIGRLGCFLSGDGDYGIPTRLHWPFGMAFPNG
                     LVPTTQTCTALGWPTNCRVYPTPLLEFLIGVAISWFLWRLARVRLAAHSTYRSAGFIF
                     GAYLILTGIVRFLVEFIRINPRSFMGMTNAQVASLISIVAGSVFIVGVGHRSSTSKHR
                     TIA"
BASE COUNT         2822 a         3062 c         3213 g         2553 t
ORIGIN      
        1 tcgcaattct tcaagcaatc aaggctgcag gcgcactcca ggccgatctt gaggtgatct
       61 aaaaatgcat attggcaatt cgcaggcgtc tcagctaagc aacgcccaaa atccgctcgc
      121 aatatcggcg agcgatccgc cgcgcgtgca gaagcttaag cgcgcagcga cagaatttga
      181 ggccatgctc ctcgccaaat ggtggtcatc aatgaaagag agtgccttgg gcgagcagga
      241 cgatagtacc gacccagggc acgatactct tgacgctatg ggcatccagg ctatgtccag
      301 tgcggtggct tcacgtggcg gcctcggaat aggtgcaatg ttggtgcaca gcttgctccc
      361 taatgttgag gaaggtagtg cggatatttc tgcggtgcct ttcgtaagga atagcaatag
      421 cgcgacttaa tcttcgatca actcaggcct gcatttggcc gatctgctag acgtgtgagc
      481 gccgaaagcg gcggtattgc attaaagtct tctcatcgat agccgataga gaagagggga
      541 gcgttaccgc tccgggagcg acattcgtga agattaattc agagttcgaa ccgcaagctg
      601 gtcaaggacc gtcggctaca ggcgcagcgg gcggcgccag tcatgttcat agctcgctta
      661 attctcatgc cggacgttcg tccgaaacgg acaaagccga cctttcgtcc gaagcacagc
      721 aattcgcaaa attaagcgct cacgcgaata aagtgcctga tgtccgcgct gaccgcgtcg
      781 caacgctgaa gaatgcgatt cagaatggtt cacattcggt aagcaatcat caaatcgcgc
      841 aagcgatggc tcgcgattcc cacccaaggg cgtaatttgc accacaggca cgcgcctgaa
      901 tcggtccggg atatcgccgg atcgacgtct tctctgatgc cggcgctttt tgtagctccg
      961 ggtaaagggc gcgaggggga gtaagcctta ggcgtgggaa aacgtgttca gggatccggg
     1021 ccggagtttc aaatgttcga ccaaacgtcc aaaatcgccg cgataaattc tttccgcgag
     1081 ccggaaactg gaacatccga gcacggacaa aaacgcaaac cgcaaggaaa gcatttcgcc
     1141 caagcggttc cagttgtcga gtcgtccggc tccgacgatg gcgacgaaca cgaattggac
     1201 actctggcat gaattcttca attgaaagcg ccgcgctact tggggcactg ggaaaacagc
     1261 tccgcctttt ccgcgagctt tcggccgatc ttgttgcgag tcgcaccgca tacggcacga
     1321 tgaatctcgc cgcaatatat cagcacattg ccacgcaaac tgcgatatgc gaggaatttc
     1381 ggcaaatcga agacgatcgc aagagtgcgt ggcgtgctgc ctgcacggca gttgatctcg
     1441 atgcggagaa aagcgatctt cgcgaactta cagctcggat ggaccggagc ttcgctgaac
     1501 cgattcgcga cgttgtaacg aagctcgcaa ttgcagaagg agagctccga aatctgaacc
     1561 gcgcgcacac tgttctcatc gaagggtcgc gccggacgct tgcgattctc gggaatgtcc
     1621 ttgcgtcctt tgcgcccact tacggtcgtc ccgcaggcat ttcggaacct gcggccgccg
     1681 ctcccaaagg cgcagttcga tgagcctcgg atcaagcctt tcgattgctg tgcaggcgat
     1741 gcttacagat cagggggcgc ttgccatcac cagcaacaac atctcgaatg tgaacacccc
     1801 tggctacaca cgccaagtcg caaatctcga ggaagccccg cctgcgcagt acgggaacct
     1861 aaatttcggt ggcggcgttg ctctggcgaa cattcagagc attcgcaaca acgttcttca
     1921 gcttcgactc aatcaggaaa cgcaagctca ggccaaactc aacactttca cgaacggaat
     1981 ggatcagatt cagactgtct tcaacgaagc ttccggtacc ggcttgcaaa gtctgctgag
     2041 caattttttc aacagtttcc agtctctctc ggtcgatccc gcaaacgacg gcgaccgtca
     2101 ggccgtgatc ggcacggcgc aaagcctagc ggcgggcttc cagcagagcg cgaccactct
     2161 ggttcagcaa cagcagaatt ccgatcaggg cgtcgtccag accgttcagc aaatcaatca
     2221 actcacagct caaatcgcca cgctcaatgg ggaaatcacc gccaccgcag gcgctggtct
     2281 caatacgaat tcgttcgagg atcagcgcgc tcaattgatt acgcaacttt cgggcttgat
     2341 cgacgtgcgg acgatttcgg caaacggcaa cactgtcact cttacgacaa ataatggcgc
     2401 cgaattggtc gtcggcaacc agagtttcga tttgcagacc ggcgtaaacg ctagcacgag
     2461 tttccatgac gtctatgcgg agggcacgga catctcctcc tcgattcaag gaggatctct
     2521 cgctggcgac atccaccttc gcgatcagga gattccgtcg ctacaaaaca acctggacac
     2581 gcttgcgtac agcatcgaga acgccgtaaa cacgcaaaac gcagctggct ttgatctcaa
     2641 cggcaacccg ggagggaaca tttttcagcc gcccaccggg gtttctggtg cagccctcaa
     2701 catgacagta atgcttacag atccaaacct gatcgctgcc agcggtgacg gaacacctgg
     2761 cgacaatgca aacgcaatag cactcgccga tttgcaaaaa cagaacatcg ttagcggtca
     2821 gcctccggtc gcctattatg cagcgctggt ctcgcagatc gggaatgacg cagcgacggc
     2881 atcgagtcaa ctcaccggcg ggactcttct gatccagcaa ttaaacgatc aaatcaattc
     2941 agtttcaggc gtctcattga atgaggaggg agcaaacttg atccagtatc aaaacgcata
     3001 cgcggcggcc tcgcgtgtcg ccacggtcgt cgcgagcctg tatcaaaccg taatcaacat
     3061 ggtcccggca agttagtggc accgtatttt gccgtgtggc accgtcgtgc tcacggctac
     3121 agccgtcagt ttcgtcgcga tcgccgttca gccgctcgcg gttttgggca gcactggatg
     3181 tttacggact ctttccgcaa actctgcgct agcagatttg tggtttgaat aactgctgaa
     3241 tcaaaaaggg aactctcatg agcatccgcg tcaacccaaa catcattccg gacatgatcg
     3301 ccggcctctc gcaaactcag caacaattga accaagcgga tcagcaaatc gcctcgggcc
     3361 gcacgatcga ccaaccatcc gataatcccg ccggaatggc tgcactaatt ttgaatcatg
     3421 atgttcagag tcagacggac actttcagca cgaacgtcag caacctgcag gatcgccttc
     3481 agacagcaga ctcggcactg agttctgcga tcacggccgt taatcaggcg atttccctgg
     3541 gagttgaagc ggggaactca actctttcaa atatcgatcg gcaagccatc gtgagccaac
     3601 tctccggcat tcagcagcag ttggtctcgc tcgcaaatac tgcgtacggg ggtacgtatt
     3661 tgttcggagg ctcgctcgtg gaaactactc ccttcgctct cgattcgatc tctccgaacg
     3721 gcgtcacgta cagcggaaac agctcaacgc tgtctgtgga aatcaatgac ggggcgaccg
     3781 ttgcgaccaa tgttccgggc gatcaggtct tcctgaatcc ttcggggagc ctcatgggtg
     3841 ccatccaagg gctgatcacc gcgatccaaa acaattccgg catcagcgcc gcgagcgtga
     3901 cgcttggaac cgccgccagt gtattcaatg gagaacgtgt gacgtacggc tcgtcgctga
     3961 ctcaattgca atcgatggga aacttcctcg ccagccagca aactcagctc gcaacgcaag
     4021 agaacaatat cgacgccgcc gaccttgcga aagcctcgtc gaacttcagc caagccagcg
     4081 tagcttatca gtctctgatc gaagcggaag ccaacattct gaaattgccg aacttactga
     4141 cgttcttgca gtagcgtgcg gcggacctca gcgcgaacga aaagtaaata cttgcaccag
     4201 agcactcgcg ctttgccgca ggggttagcg ttgccacaac agcaaaacgt cctgagttct
     4261 gtttcagcgg atcagtgcct catacatgac gatcttccag cttacgtggc gcgccagcct
     4321 gaactgcgac gcgctcagcg ggctaggccc aactcgcaaa ggaagcccct gatatatcat
     4381 tgaacgactc tctcggtgcc agcacctccg cgtgctttcg cgtcttcctg gaatggtgtg
     4441 tcgctgattc gacgggaaga taatgtggct aaaattcatt tcagacggcg ccaacgatcg
     4501 tgagcacaga gaatgttgag aaacgtctcg tagatggaat gccaccatgt cggaccaaaa
     4561 gcgccgctgc tcaatccgcg caaaatgctc ttcggcattt gccgagattt tttaggagca
     4621 cccagctttg agacgtcatg gccgtttcgc aagtagaggc tgaaccctgg tgccctgttc
     4681 gtcgtcgcta ggctgaccat atcgcaccgc tggatgctcg cgctggtacc tggagtcatc
     4741 gccatttccg cgaaaattag cgccactgat gaacggttat gtatagttta aaatagtcgt
     4801 cagcaatcac catcgcgagg aaaacagctg ggaggctgca gcaagcttca ctcttgccac
     4861 tacgaaaaat cgcaaaccca acgcaagcct ctaaatcgtc gggccaattc gtgcgctcat
     4921 gccagagccc tcgatccttg ttcacttcgc acccagaaca cgcagccccc ttgatcctta
     4981 ttcacctacc ccggggtacg caaaccattg atcctttctg ctcggcacag aggctctcga
     5041 cccgttaatc ttggtcgctc cacaaaggcg gcgcttaacc cattgatgta catcttgatg
     5101 aacatccgca tgtgcaccac gccgtgtgat gtgcacctgc accacgccct cgcgagtggc
     5161 aaagctgccg ccatatcgtc gcgcacgtca cgtgtgcttc cacatccttc agctccacgc
     5221 gtcgcaagac gtcgtgtccg atcacgcttc gaagaatcca cgcttcggcg tcgcgagcat
     5281 cagtgcttgc atcgcaatcc cgtgcatcgc acgtgctatt caacgttgta ccgcgagccg
     5341 caaaaacctt cgtccgcagt ctgaatatta tatgttgtgg ggcggggcct atgggtgccg
     5401 agatcttgtg aatttttatg gatgatttag gaaagagata agcgcgcgca atttatgcag
     5461 agcgcgcgac gggccgtggg tcgtcggtgc cggttcgcag cgtgattaaa gaatgatcga
     5521 cgggctgatg gaaagaaatg aacgaggctg ttggtggttg tcgatgcggt aatcgagagt
     5581 atgtcgggta cggaccttct tgttgttggt tgcgagggtc cttcagcctt cgtgctctgc
     5641 gggcgcgtta tttgcactgt tcaaaaaaac ctctgagcaa catttgcgcc cggttgtgta
     5701 acggagtcac gcggggcgta cggcgtcgag gcgaaaggct taaggacggg atcaatgcgg
     5761 tgcggagagg ctgcaattga gacgtcggct aaaggatgta gatgcgggaa taaagatctg
     5821 agcgggagga cagcggctag tccatgtgat ttagctccgt gctcgttgaa gacagcgtca
     5881 agcgtgatgt caaaacagcc tcagccttct cctagccctg aaacttcgga tttgcgaaac
     5941 ggagttctgt tgttttcggg agtgcttggc aagcgtgccg aaccgtgttt cttggggcct
     6001 ccgttcaagg agccgccaag gtgcgcgcaa tttgaacgat ttggccgccg ccgccgtcga
     6061 tggccacaag tagtcaatgt aaacaacagt gctttggata gagagctaag ggagacactc
     6121 gatagccgtt ctcagattga gtttgcgctg tatctcataa aggaaaggac atccatgtct
     6181 ataaaacgct tcatcgcaca ggagcaggaa attaattgtg gcactgcggc tacacaatct
     6241 gtaagtacaa gctggttcgg gcgcctctct gctgaaatcg tacatcgatg tcgtctcgca
     6301 ttgtttgccc cgactgttca acgttcgctg gcggttgagc gggagcgccg acagatcgag
     6361 cgtgaacaga tggcggagga atacgcgcgc ggctaccttg aaggatggca ccagtgttat
     6421 tcggcctgtc tggaagctgt cgaagaatcc gtcgccgaaa accgcgacat ttggaccgcc
     6481 ggcgaagtgc ttgttggttc cagagcgaat tagaacttct ggtcaccgat taaaaattac
     6541 ccatcggcaa aatgggcggg agccgcctca agaggagaac aatgagatcg aaagaggtgt
     6601 ttgaagtgtg tcacggcaaa tataaagggc tacgaaactg gctttcggat tctactggtg
     6661 ccgtaaaatc agtcgacctc gatttgccat tggtcggcta tcagtggaga ccaaatcgtg
     6721 gcaaggcttg tgaatacgtg gctgatttcg aacgaattgg acgacacggg cttcgcagac
     6781 cggagtggaa gggcaggcta cgcctattcg aaacttattt tgtgacggga atggactacc
     6841 agaaagcacg ttccgcagtt ggcgtggccg aaggtacgtt cgactattgg taccaagaag
     6901 ttaaaaagac cctgggagca gaattttcga ggacgggtct gtatcctccc tcgaagtatt
     6961 ttcacgctta acggcgactg ctcccacggt gacgtctcag tccacgggtg gggcaggcct
     7021 tgggggagac ggcacctgga ggcccacttg gttcagggga acgagcaggc gcaccagccg
     7081 ctataccgcc gcattagtac ccgtgctaaa ttctgtgatt ttagcgtttt acggatggga
     7141 tgcggcatga tatcctacct ctgcgcaaca tagttcggcc atggcaatgg agaatggttg
     7201 tcgcctcatt ttttgatcgc catgcaataa taatgaacgc ttccttatgt gaaagaaaac
     7261 aataggctta gcttggacgc ggcattcagc agttccggag ggaggaaatc ttgcagattc
     7321 tgacggaagg ccagaccgtg cggcacgaac aatatggcat tggaaccgtt acggagtcca
     7381 ataatgagag aaccaccata gatttcgaca accacggcct caaaaagttt gtgacgtcga
     7441 tctggacggc ggaactgatt ggcgaaccgc ctgccgaccg tgcgccgcgg cggcgaggtc
     7501 gtcgcaaggc gcctaagaaa gcctaagaga gtgcgccccg tatgtctgat gctgggcttt
     7561 ataggtcaac taggttattc cgcacggcaa agcggacaag ttcgctgaag ttttggaatc
     7621 ccattttgtg catgatgtga gtgcggtgac tttcgatggt acgtatcgaa acacccagtt
     7681 ccatcgccgc ctgtttattg ctctgtcctt cagcaagtaa ctgtaaaacg tcaacttctc
     7741 tccgcgtaag tggtgagcct cggagtggtt ttcgaccggg cgcctcctgc ggcttggctg
     7801 gcccatgcag gaagctatgt gccatggtga gtgctaatgc gccagtaaag aaaggctgct
     7861 tgtggcgaac gtggtcgatg gctgcgagaa gttcgccatc ggcgtctgac tttagaacat
     7921 atgcgagcgc gccggcccga agaacctccc gtgccaactc ctcagagaaa tgcattgtaa
     7981 gcacaacgac tgcagtggag ggggatgtcg cacgaatatt gcgggtcaca tctaagccat
     8041 tcatgtcagg catggtcaga tccagcacaa ccagatcggg acggaacttt cggacacaat
     8101 ccagcgtctc gggcccggaa gatgcttctg cgcagacttc caatccgggt tgggcgtcca
     8161 ataaagcgcg aagacctcga cggacgactg catgatcatc cgcaacgacg atcttgtaat
     8221 tcttaactat ggcggtcacg gtattctcaa ccgatgcgcg acgaggcctg cttgtggcgc
     8281 gtgcgtattg gcttcttttc gcgaacagtt ttggagctgg aatctgctgg caatgtggtt
     8341 cggatagttg ttcccttgcc agctgccgtg taaatttcaa aagttccatt gagttgttgt
     8401 actcgttctc tcattccaag gattccgact ccgagtggaa aatctcttcc atgcgtcgta
     8461 ccagggtttg acaagccgca gccgtcgtcc tggataaccg catgaaccaa gccatcttcc
     8521 actgtaagcc gaatgctagc cgtgcggctg cctgaatagc ggtgaatatt cgtaagtgct
     8581 tcctgtacga tgcggaatag ggtggtttca atttcttgcc tgtgccgacc gaagtcttca
     8641 ggaataaaaa cctcaatagg aataccggat cgttccgtga agccctttgc ataccagcgc
     8701 actgccgatc gcagtccggc ttcatcgagc atcggcgggt gcatcagata ggagagggtc
     8761 cgaatttcgc ggatggcagt ttcggcaagc tgaacggccg attcaagaaa cttgtttact
     8821 tgatcgtcac tttctggaag gacgtcccta aggcgaccaa ggctcatttt tacggcggca
     8881 agcgtttgcc ctgtcgactc atgaagctcg cgagctatag cgcgacgctc atcatcacgg
     8941 gtgcgcagga gcttttgaga gagacggcgg agctcacctt caatttggtt ttgatcgctg
     9001 aagtcgaagg taatcccgac aatcgactta aggccgccag ttacttcgaa attcggaagg
     9061 cctcttacaa aatggtgccg ggtgccgccc atcggcgaaa gataacgagc gacatactga
     9121 aacggtgtcc ccgcagcgat cgcagatctg acgctttcaa gaacggacgt acgatcagcc
     9181 gagtggatcc tcttccagta tcgatcttcg gtcagacaga tctctggggc aatgccaagc
     9241 atttgcgcca aattgggcga aagagtggca atgcgtgact cgatatcaaa ttcccaggtg
     9301 ccaaatctcg cgatttcttc ggcttgcgtt aagaggactg ccctttgcga tggaatttcc
     9361 tgcgaggctg aagatttggc gcaacaaaat cccaccacgc ttacaacctg acctgaacgg
     9421 gcgcaaattg gcaggattct aagtaagaga tgccggtcac caccgttctc ctgatggaga
     9481 gtgatatcgc atgcgcaaga tatgccagac attgcggcct tcacattttc cgccacgatg
     9541 tctcgatctt gggcactaac cagctgtaag aacgtgtcaa tgccatcagc gcagctgtaa
     9601 cggcgcctag atgacaactt aaactgatca atacaccaaa caaaagtgtt cgatttaatg
     9661 tgccagtgcc acgtgtatat tccaatcaaa tcgttgttga ttgaatggct tgatgaagcg
     9721 gaaagcgcat cggtccagaa aatatttata agtgaatccg gcgattgtcc cggcataggc
     9781 gcagacaatg taaaggcaag aacttaacgt gtcaatgtga catattctca aaatgaacag
     9841 tgatccgtaa tactactgat aaattgcgaa agaagccgta gttttgcgga ttgctttctc
     9901 ggggaggaat attctattct cagttcaatg ctggaaggga agtagcttag cggcgcggcg
     9961 gatcctatga acggccttga ggggttcgcc gccgtcgcca cctaacgggc tgcaaacggc
    10021 aaacttaagc gattgttctg tgcttagacg tggatgatcg gtggccgacc cccacgatga
    10081 atacagaacc cgccacaatc gaaatcaggc tcgcaacctg cgcgttggtc attcccatga
    10141 aggaccttgg gttgattcgt atgaattcga caaggaaccg cacaattccc gtcaatatca
    10201 agtaggctcc aaatatgaat ccagccgaac ggtacgtcga gtgcgcggct aggcgtactc
    10261 gagcgaggcg ccacaggaac caactaatcg ccacacctat caaaaactcg agcagaggag
    10321 tgggatagac gcggcaattc gtcggccagc ccagggctgt gcaggtttgc gtggtgggga
    10381 cgagaccatt gggaaatgcc ataccgaaag gccagtgtag acgagtgggg atcccataat
    10441 cgccgtcgcc cgagagaaag cagccaagtc ggccgatgcc gtaaccgagg gcggctgcag
    10501 gcgagccggc atcgagggag acgaggagtg ggatgcggtg gcggcgagac aaaaagatcc
    10561 aggcggccgc gcctgcgata aggccgccga accacgcaag tccataacgg ctaaagagtt
    10621 ggccccaagg gtcggcgagc agctcgcggg gcgtctcgag gacgtgataa aggcgggcgc
    10681 caatgattcc gcagatgcat gggacggcga tgaagagctc ggcgagattc gattcggagc
    10741 ctttggcgat gccacggcgc gcgaaatcgg cgcgcaagac gaagtaagca aagatcatag
    10801 cggtggcgac catgaggccg taggtgggga tggtgatcgg gccgagatgg agaaacggga
    10861 tcatttttcg gtgcagggga atagactagc aggatgtgcc gctcatcttt cagaaaagtg
    10921 cgcgtggcaa attcggccac gaatgcgtcc tataattcga ttagttggga accatgggac
    10981 ggtttttgcg gtgttggctc cttatttttt gaaaagttca aaacctggat tgcagagtgt
    11041 agcgggctcg atagagtggt aaaaatttac tgtcgagaaa tcggtatcgc gagcgcgatt
    11101 gccttggacg attaaagttt ttagaaaaaa gtacttgcat tgttttacgt gttctgctat
    11161 aatctttttc gttaacctcg aagagtgcgc aactgaccta gcggtacaag aacccaagaa
    11221 aatgtaaagc agagggatgc gagatggcaa acacatctgc ccagcgggcg aagtgtgcct
    11281 ccgcaccgtc cacatccaat tagttgagga aatttattga gaatcgaaag agagtggcga
    11341 gtggctagtt tgactgctcc accgttcacg gtggcttact taacgccctc ttcggcagcg
    11401 ccgcatttac gacatcgctg agagccacgc ccttcacttt ggtgagtttt tccgtcaatt
    11461 tttcatctca cagggacttc gaagtgcaat gcaggatctg atgaccgaga tccgcaatat
    11521 cacgattggg agagatttcg atgccgaatt acaacgcgca ggtaccaccc tattcagtca
    11581 atccgggcga tgtggccctg gcttttaata atgagtctcc gacagcaggg caggcgagcc
    11641 agcagtttgc
//
LOCUS       DATNDT010000103        23622 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3212727, whole genome shotgun sequence.
ACCESSION   DATNDT010000103 DATNDT010000000
VERSION     DATNDT010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 23622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23622
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3212727"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..2973)
                     /locus_tag="VN788_03170"
     CDS             complement(160..2973)
                     /locus_tag="VN788_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU19566.1"
                     /translation="MNEHLRTRLGAVALAVLTLAAVVFAVLNYQQRSRFIQPDDGVTW
                     MDSTQGVVAWHVVPGSPADRAGIQQGDYVESVRGVEVHRATDVTRLLWRAGPWMEVKY
                     GIRRDGQTLQVPLVTAPQENPSSLEDYLRITALLYLFIGLFIFVRRWNAPRAVHFYVF
                     CLVSFIFYSFHYSGKLNSFDWTIYWSNVVALLLQPALLVHFALVFPERRGAVWPKVAA
                     LYSIPAALLGLHVFVATGTLDFLPSISNRDFLDTIELLYLGAYFLLAAVIFLASYFRA
                     PSGILRQQLKWVTGGTFAGILPVFLLYILPRLVSAVPQPWMKLSVFSLVLIPLCFGYA
                     IIRYRLMDVDIIFKRGLAYTFATVGLVAAYFAVIALIGELFHTAWPTGPAGAVIAIVA
                     AAFLFQPLRDWVQARLDHFFYRDRLDYRRTLIEFGRTLANEVRLDPLLDSVLDRISQT
                     LLVDRLAVFLEDPANPGQFLLSRSMGVKRTDALNLSFLDPSRSGFEQGCLFFESARAA
                     RESESIRRTLEELELNYFIPCRFRDRTAAFLGLGKTVDGDYLSSEDLELLSTIAGYVA
                     IAIENARLYQSLEQKALQIERLKDFSENIVESLRIGVFTIDLEDRIESWNPQLEELLA
                     MPRSEALHRKMEEAVPRDLAAEIAVSSPSEHVAGIYRLHLDTRVGRHLVVNASVAPLL
                     GKDGSRLGRLILLDDITQRVRLEDQMVQTEKLTSLGLLAAGVAHEVNTPLAVISNYAQ
                     MLAKQMPADDPRQKTVDRIVKQTFRASEIVNNLLNFSRTGGAESVELDLNAVIDETLT
                     LVQHPFKTAHVTVLKNYAEKLPPVLGSINRLQQVFLNLLMNARDAMPTGGMVEVRTGA
                     HNGSVEIEVSDTGGGIPPENLHRIFDPFFTTKASGCGTGLGLSVSYGIIKEHAGKVDV
                     RSTPGKGTSFRLEFPVARKSVHA"
     gene            complement(3150..4802)
                     /locus_tag="VN788_03175"
     CDS             complement(3150..4802)
                     /locus_tag="VN788_03175"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU19567.1"
                     /translation="MAAAPNTGAISGVVVDGTGTPQMGAAVLISSEKIAASVPLRLLT
                     NDLGHFATPSLPVGMYTIHVTLAGFLPAVEQHVRVNGEQATLLEIVVDSVFSSFEKLR
                     RQPDDTVSSDDWTWVLRSAAATRPVLRWQDEDDTAVAASTEPADTPSDEPLRHGRVEL
                     TSGADGAGSIANLANAPATAVVYDLGLGEQGDLVVAGQFSYSGVDTSSGLAGEWRPNG
                     PAGAGPLTTFLVRESRIGPNGSVFRGLRLTQDNQMALGDRVSVRYGAKFIVAGFNGTT
                     AAVRPHGEVAVQVAKTWRASIILATDPWADPASGDGAVDSTLNTLDAFPSLMLHDGRP
                     VLENGSHQEIAVEHSLSRRADITASFFHDRSTHTAVIGRGGVSNPDFLQAYLSESFAY
                     DGGSTGSMGGRVVYRETLPKNLKTTLVYSYAGALAPSGGADGLALRDELATRYRQSLA
                     AGISGKSRHFGTQISASYKWLDGPAVSQQDPYGESLYHMDPYLSFEIRQPLPAVFPGH
                     MQLEANVGNLLEQGYVPVATRDGYVVLIPSYRYFRGGLSVQF"
     gene            complement(4871..6598)
                     /locus_tag="VN788_03180"
     CDS             complement(4871..6598)
                     /locus_tag="VN788_03180"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HXU19568.1"
                     /translation="MGQPAKTLLGLFLAACVSTIPIHLAKAAQQDNQDQQQDQQADQN
                     HGKSSKAKQKSLKDLYKELNSQYKKWLNEDVVYIISPEERRSFLQLQTNEEREQFIEA
                     FWQRRNPDPDSPENTFKEEHYRRIAYANEHYASGIPGWKTDRGKIYIMWGPPDEVDSH
                     PTGGQWDRPMDQGGGSTTTYPYEDWRYRYLEGMGENVELEFVDPTSTGEYHLTMDPSE
                     KDALLRVPGAGLTLAESMGMASKNSRFNNTDGTNMAEGQLGGLSEKDNEFTRLENYAN
                     IMKPPPVKFKDLEAVVTNRLVRDQVKFDYRYDFVRITSDTVLVPITVQIPVRQLSFQE
                     KGGVDSATMDLFARVTSLSGRIVQTFEDTLRADFPSGQLQKSLSTSRIYQKAVPLSPG
                     LYRLDIVIKDVNSGNVGVVNTRLPVPRFQDDQLSASSLILADDIQRVSSKDIGLGQFV
                     LGDVKVRPRMDGTFTQKDPMGVYLQVYNLKVDDKTHKPDTSVEYRVMQGKGTAPVLKF
                     DVPADKLPQHGEEMTLENLITLGSLAPGKYHLEVEVTDNLTKQTITPTADFTVKAAPA
                     AAAVTAQGR"
     gene            6951..8342
                     /locus_tag="VN788_03185"
     CDS             6951..8342
                     /locus_tag="VN788_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU19569.1"
                     /translation="MKTWQKATIAACVVVLIGGIAWYSIYKSNQGVTAVQTARAARED
                     LVSLVTASGEIRPKNYTNVLGEGIGKITDIAVKEGDHVKKGDILLHLENVQPGADVQA
                     QEDSINATEAGLKAASASYDVAVATLAQRQADLDKAKLDWQRGQELYRGKLIARQEYD
                     SDKAAYDSAVASVNSSQAQVDQARAAREQARATLDQTQAVLRRTQDILRKTTYRAPID
                     GIVSYIAVRVGENVVPGIQGTVGSSIMTISDMSVVTAEVKVDETDIVSVKDGQPADVS
                     IDAIPGKTFRGHVTEVGELAILRTSGNASMTETTANTQEARDFKVVITLDNPPNTLRP
                     GLSLTAKIQTAQKQNVLTVPIQALAERSQKELDEAKNGNKGSVTLAASTSEGASSVKA
                     DIQGVFVLRNGKAEFVPVQTGITGVTDIEVTSGLHEGDLIVTGSYKAIRTLKPGAPVK
                     IDNSVSQTDTSSS"
     gene            8371..9123
                     /locus_tag="VN788_03190"
     CDS             8371..9123
                     /locus_tag="VN788_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU19570.1"
                     /translation="MAVEEVGSQSFRQDLVDSGIVIHTEDLWKTYEMGTEHLHALRGV
                     SITIHKGEYVAIMGPSGSGKSTLMNLIGCLDTPTRGNYWLAGRLVSQLDDDELASIRN
                     QEIGFVFQTFNLLARATALHNVELPMIYNGTPSEERLDRAKKALAQVDLSERMMHRPN
                     ELSGGQRQRVAVARALVNNPSILLADEPTGNLDSATGAEIMALFARLHQTGNTIILVT
                     HEADIAEHAHRVIRVRDGKVERDEPTPHLPPD"
     gene            9474..12785
                     /locus_tag="VN788_03195"
     CDS             9474..12785
                     /locus_tag="VN788_03195"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU19571.1"
                     /translation="MTRLGAQVANTATIQGTVTDASGGVLPNAQVQVTNTGTGLVRTT
                     TADAQGRYSVLTLQVGTYDVQALATGFQKVTHTGVLLTVGNIQTVDFSLPVGTAQETV
                     TVEGTATQVQTSDTSIAHLVEPTQMTQLPLNGRNFSSLLLLEPGIQSTQSGSSFYGTQ
                     NNYTIAGQRPQGQGFLLDGSDVSTFWDHGTGSGATGNSLGIDSIAEFQALTNTYSTQF
                     GGSGAVVNATTKSGTNGFHGTAYEFLRNSALDARNFFDPPKIPEFRRNQFGGSLGGPV
                     VKDKAFFFVNYEGLRQSLGHTEVANAPDLAAHQGYVPNSMGVLVGAMPGCPAYSPALP
                     MNTCVAANVQSTLALFPVAQNDSVAPGVGTVAEVASDVANENYLVARFDYTLSEKDSF
                     FLRYIRDTASQTLPFSGSPIPLWPEGDTTANHFATIGERHIFSNNLINSVHFEFDRPK
                     DVGNTTSSQAATKYFDGREDGNCCGGFGGSFPLSPIGANALLPFSLPQDKFVFADDIL
                     WTHGSHSFAIGAAVKREYTYTFAPFEWGGSWTFANLQAFLGGQASSLFSAAAGNVISN
                     KDFRELFVSPYFNDTWKVSSKLTVNYGVRWDWETDPIQTVYGMQAFPNPPVGSSPGCL
                     GGGCFESVTHAFASNFNYKNWEPRVGIAYDPFGDHKTSIRAGFGLFDDVVMPREYMPG
                     YWLDPPFQLAIQINPPYPTPFGSGGFSFPLAPIQPEGQVYAWKYTPYSLQYNLNVQRE
                     LFRNTVLTIGYVGSQGVHLTKANDVNPPGMIVNGKAVPYINNGTQWPVQRGGGLCTTL
                     PPPDGQIVFGCLDATGLSVDPLQQANPTLGQLSEREPVAHSHYNSLQASVNRPFTRNV
                     QTQLSYTYSKCIDNGSVSSGLEAFNLAAQLFTNPYNLALDVGPCDFDQTHSFRGSGVV
                     GLPFHGNLFVEGWQLSGILSANSGYPINISTGYDISGEASGDRPNLASGSPRPTSLGH
                     PAQWWAFPANMTMPLVGELGNVPRNSALGPGFLNFDFALLKDTPVRKISEQFRIEFRA
                     EFFDIINHANFGTPNGGLFIACSTTDPACTPAFAGANGGFERSSTFGLVQTTVGNAST
                     GGSERVIQFGLKLLF"
     gene            complement(12853..13812)
                     /locus_tag="VN788_03200"
     CDS             complement(12853..13812)
                     /locus_tag="VN788_03200"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXU19572.1"
                     /translation="MGIAPMIAASGPRSSTVTPKNEIFGARETHGRALKAENRLIAHS
                     PEVGWRSLYAAIIEEGPLEAVETPIPHPFLIYHLYHPTEVTRKIPGAHVERALIGPRR
                     ICVTPGGTAARWQHHGHPEILQIYLRQSIYSAAVNEMYGCDASAAEIVPRFAILDPLL
                     EQLALTIATGLRDNSVRDGLYIDTLAQMIAVQLARYHSSRSGPSNRAIANAAPTWKIR
                     RLMDFIEENLDRDLSLEALAAEVNISPLYLPRAFKSAVGQSTHQYVLQRRVERARKLL
                     GNSDMPIAEIALCSGFSSQSHLSDWFLRLVGVSPAAYRRHGLS"
     gene            complement(14044..14928)
                     /locus_tag="VN788_03205"
     CDS             complement(14044..14928)
                     /locus_tag="VN788_03205"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXU19573.1"
                     /translation="MQGLYFWVRSFAIVGALIFCTMMVGVPSQAQSVGGVQLSAGSAS
                     TPGQLPDILKGVYFEQRLGKQIPLDATFTDENGNPVVLRSYFEKKPVVLIMAYYRCPM
                     LCSQVLHGAASAFKKLNFQIGDQFNVLTVSIDPQETPALAASTKRTYIKQYGEPSAAA
                     GWHFLTGKKPQIDSLANAVGFHYKYIPQLNQYAHAAGIMVLTPAGKVAQYFYGIEYPV
                     QDLRLALVQGSHEQIGSVVDQILLYCCTYDPGTGRYHALVGRLLQIAGGATILLIASA
                     LFFLFRWDARRKSHGTPA"
     gene            complement(15379..16305)
                     /locus_tag="VN788_03210"
     CDS             complement(15379..16305)
                     /locus_tag="VN788_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HXU19574.1"
                     /translation="MKWARQILIAASENAWLRKTMPRLPFVRRAATRFLPGENMGNAL
                     AAASRFSRAGISSLLTHLGENVNERAEAQSEIQQYLNLLDRIQQGGLPSEISVKLTQL
                     GLDIDAEFCFANLVALAEHSPHKTIWIDMEQSRYVEATLELHARARRTHANIGVCVQA
                     YLRRTEQDVESLIALGSSVRLVKGAYSEPPEIAFAKKKDVDENYFRLAQNLLSSRARE
                     TGVRVVIATHDTKLVARIAEWASAQSIEKSQLEFAMLYGIQRKEQFRLARKGYRSDVL
                     ISYGSFWFPWFMRRLAERPANIFFLARNLFSG"
     gene            16479..16787
                     /locus_tag="VN788_03215"
     CDS             16479..16787
                     /locus_tag="VN788_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family peptide modification chaperone"
                     /protein_id="HXU19575.1"
                     /translation="MASMDARLSIDSIVSATSEQLSCALGEESAILNLKNTVYYGLDP
                     VGTRIWNLLQQPIGVRELRDRITDEYEVDAKQCEGDLLELLTTMQNEGLIRVEGSDRG
                     "
     gene            16851..17276
                     /locus_tag="VN788_03220"
     CDS             16851..17276
                     /locus_tag="VN788_03220"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HXU19576.1"
                     /translation="MRGFALEAASVLTITWLGLRSLGFRRWQRLLTRVMPAAPNTFEA
                     SESLLDRCRAIARIEQSVARRLPFQSNCLDQSLVLWWMLRRRGMAAEMHIGGRKDAAN
                     FEAHAWVEVGSYTFPEMSGGHRPFTPFDGPIESVETQTR"
     gene            17273..19120
                     /locus_tag="VN788_03225"
     CDS             17273..19120
                     /locus_tag="VN788_03225"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HXU19577.1"
                     /translation="MSGIVGILHLDGAPVDAELLQRLVDFQKFRGPDAQQIWISGPIG
                     LGHTLLKTNDEPVHECQPFHLGDDIWIAADARVDGRSDLAAKLTAAGQKHPPDATDAE
                     LILRSYRAWGEACLEHLLGDFAFAIWDGREQKLFCARDQMGVKPFYYANIGSLLVFSN
                     TLDCVRQHPAVSDRLNDLAIADFLLFETNQDAATTAFADIRRLPAAHSLRCDRGAVSI
                     RRYWTLSVTEQIRFKQDAECIERFRVLLDQAVADRLRTKSAGILMSGGLDSTTVAATA
                     RRILTASGNDNGLFAETVVLDELTPDNERHYATLAANALKIPIRFQTADHCGLFKGAE
                     NPEYRPPFPEHSAWPDQTRDLLREIGARSRIALTGSGSDPGLSSRITVHFRQLLRKRR
                     YVRAASDAVRYLSAEGRFSRLYLRARWRLLVSSTNAFSSYPNWLNEDFQREWNLEERW
                     KAYGVSDRATQLRTESNVNAFRPEALTSMIHVSWQDLFETLDPSATRVPIEVRHPFFD
                     LRVVTFLLGLPRLPWCCDKELLRQAGRGILPEAVRIRRKSPVENDPIVALLQKSESAW
                     VDRFEPVPELGRYICQHRIPPVWMEKSSGTAWINLKPLSLNFWLRTGKN"
     gene            19504..20529
                     /locus_tag="VN788_03230"
     CDS             19504..20529
                     /locus_tag="VN788_03230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19578.1"
                     /translation="MAFHRLYGLLLETNIAIPGVPVLPYADGIIDVQIRLKSERSPLW
                     SSKLPSEAFYVSRMRDASGNPVLRAGLLGDARHLSLVYSDGTHFLLEDRGKEIFADWP
                     DPLTLEDASLYLVGPILGVVLRLRGMVPLHASAVSIGDQAVALVGPAGAGKSTTAAAF
                     ARCGYRVISDDVVALGQEASRFAIPPGYPRVNLWVESVKAMFGPGASLPPICPTWDKH
                     FMPLDPHKEFEARSLPLGGIYVLQKRAAGITGPIVEQLTGTEAFVALLGNTYMNYLPD
                     PDKRCREFELLGRVVGEVPIRRIQAAADLSMLPELCATIAADMENLLAARQLEKAPSS
                     ALQAGPC"
     gene            20989..21519
                     /locus_tag="VN788_03235"
     CDS             20989..21519
                     /locus_tag="VN788_03235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU19579.1"
                     /translation="MTRRLLSICFFIAIFGLFSGSRAKADDLFTYQFGGNTFTWELPA
                     SPTVVIPPDMATAGMFFELVGVPFSENGGAQTQGTLDFFQAPDNGGGFQLFPMGNSAA
                     IVNAFGPQLYNGGEGSPTFKLGTFALNNGGADGPLGTLTINSFSTPEPSSLLLLGAGA
                     LALLGLTRKRIFAHQM"
     gene            21901..22569
                     /locus_tag="VN788_03240"
     CDS             21901..22569
                     /locus_tag="VN788_03240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19580.1"
                     /translation="MQMTKLAGICALLAVFAATTAIAQDQTTSDQTEPASTPSAPQAP
                     KKVKRVYTAWKYEASAGFAHRSYYAPGGGTFGLVGWYASGEYNWKKWLGAEGEVTDTW
                     YTLKSPGFPRDTIYIYTFLAGPRFYPLGHHKVTPFGHVLIGAGHYSSATPAFGGFHGA
                     TLTSNAYTYEVGGGLDLNLWEHWGVRMIQADFGSANYVNNPNYTNRGSTRFSVGFVYH
                     FGAR"
     gene            complement(22598..23185)
                     /locus_tag="VN788_03245"
     CDS             complement(22598..23185)
                     /locus_tag="VN788_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HXU19581.1"
                     /translation="MPFINFPAREINCKLVYYGPGLGGKTANLQWVYDHTGTSQKGKM
                     VSLATETDRTLFFDFLPLDLGTVRGFKTRFHLYTVPGQVFYEASRRLILKGADGVVFV
                     ADSQEERMDANLETLDNLREHLKDHNLDFTTIPYVLQLNKRDLPNVLPVDELQKQLNV
                     KGEPVREAVAITGEGVFDTLKEVAKLVLAELKKNS"
BASE COUNT         5211 a         6841 c         6501 g         5069 t
ORIGIN      
        1 ctcacatcca gcagcatcag atcgaacggc cggtcgtcaa gcttttgcaa acccgccgcg
       61 ccggtctcag ccagcgtcac gtcgaagctc tcggaagtca gcaacgcctc gaggccttcg
      121 cggatttcaa tctcgtcatc gacaactaga atggaccctt tacgcatgca ccgatttcct
      181 tgctacgggg aattccagac ggaaggaagt tccctttccc ggggtggagc gcacgtctac
      241 ttttccggca tgttctttga tgattccata gctcaccgag aggcccagtc ctgtgccgca
      301 gccgctggct tttgtggtaa aaaacgggtc gaagattcga tgcaggttct ccggcggaat
      361 gcctccgccc gtatcggaca cctcgatctc caccgaacca ttgtgagcgc cggttcgaac
      421 ttcaaccatg cccccggtcg gcatggcatc gcgagcgttc attagaagat tcaggaacac
      481 ctgttgcaac cggttaatcg agccgagaac cggcggcaat ttctcggcat aattcttcaa
      541 gacggttacg tgagccgttt tgaatggatg ctgcaccagc gtcagtgtct cgtcgatcac
      601 cgcattcaaa tccagctcga cggattccgc cccacccgtg cgcgagaagt tcaaaagatt
      661 gttgacgatc tcgctggcgc gaaacgtctg ctttacgatc cgatccactg ttttttgccg
      721 cggatcgtcc gccggcatct gcttcgccag catttgcgcg taattggaaa tcactgccag
      781 tggcgtattc acttcgtgcg ccacgcccgc cgctagcagg cccagcgagg tgagtttctc
      841 ggtttgcacc atttgatcct caaggcgcac ccgctgggtg atatcgtcga gcagaatcag
      901 gcgtcccagc cgcgagccat ccttgccgag caggggggca accgaggcat tcaccactag
      961 gtggcgtcct actcgcgtat ccagatgcaa ccgatagatg ccggcaacat gctcgcttgg
     1021 cgagctaacc gcgatctccg cggcaagatc acgcggcacc gcttcttcca tctttcggtg
     1081 cagcgcctcg ctccgcggca ttgctagaag ctcttccaat tgcgggttcc acgactcgat
     1141 cctgtcctcc agatcgatcg tgaatacccc gattcgcagc gactccacga tattttcgct
     1201 gaagtccttc agccgctcga tttgcagcgc tttctgttcg agcgactggt acaaccgcgc
     1261 attctctatc gcgatcgcca catagccggc aatcgtcgaa agcaattcca ggtcttcgct
     1321 ggaaagatag tcgccgtcaa cggttttccc cagtcccaga aacgccgccg tgcgatcacg
     1381 gaagcggcaa gggatgaaat aattaagttc cagttcttcc agcgtccgcc gaatggattc
     1441 gctctcccgc gctgcgcgcg ccgattcgaa aaagaggcac ccttgttcga aacccgagcg
     1501 cgacggatcg agaaaactca agttcagcgc gtccgtgcgc ttcacaccca tcgaccgcga
     1561 aagcaaaaat tggccggggt tcgcgggatc ctccaggaaa acagccagcc ggtcgaccaa
     1621 caaagtttgc gaaatcctgt cgagaacgga atccaacaaa ggatccaggc gaacttcatt
     1681 ggctagtgtt cgaccgaact caatcagcgt gcggcgatag tccagacggt cgcggtaaaa
     1741 gaaatgatcg aggcgcgctt gcacccagtc ccgcagcggt tgaaaaagaa aagccgccgc
     1801 tacgattgca atcacggctc ccgccggccc tgtgggccat gcggtatgga acagctcgcc
     1861 gatcaaggca atcaccgcga agtacgccgc caccagcccg accgttgcaa acgtataagc
     1921 gagtccgcgc ttgaaaataa tatccacgtc catcaagcgg tagcggatga tcgcgtagcc
     1981 gaaacacagg ggaatcaaaa ctagcgaaaa aacggagagc ttcatccacg gttgtggaac
     2041 cgcgctcacc agccgcggaa gtatgtacag caaaaatacc ggcagtattc ccgcgaacgt
     2101 tccgcctgta acccacttta gttgctgcct cagaatgccg ctgggcgcac ggaaatagct
     2161 cgccaggaaa atgacggccg ccagcaaaaa gtaggctccc agataaagga gttcaatcgt
     2221 atccaaaaag tcgcgattgc tgatggaggg caaaaaatcc agagtgcccg tggccacgaa
     2281 cacgtgcaag ccgagtaagg cagccggaat actgtacagt gcggccacct tcggccacac
     2341 cgccccgcgc cgttccggaa aaaccagcgc gaaatggacc agcaacgctg gctgcagcag
     2401 cagtgcaacc acgttgctcc aataaatcgt ccagtcgaaa gagttcagct tcccgctgta
     2461 gtggaaacta taaaaaataa acgaaaccag gcagaaaacg tagaagtgaa ccgcacgagg
     2521 cgcgttccat cgtcgcacaa aaataaacaa tccgataaaa aggtaaagca gcgccgtaat
     2581 ccgcaagtag tcttcaaggg aagagggatt ttcctgcgga gcggtgacca gcggtacctg
     2641 caaggtctgg ccgtcgcggc ggattccata ctttacttcc atccaagggc cggctcgcca
     2701 aagcagccgc gtaacgtccg tggcgcgatg gacttctacg ccgcgaacgc tttccacgta
     2761 gtcgccctgt tgaatcccag ctcgatcggc tggcgagccg ggcaccacgt gccaggccac
     2821 cacgccctgg gtggaatcca tccaagtaac gccatcatcg ggttggataa atcgcgaacg
     2881 ctgctggtag ttaagaacgg caaaaaccac cgccgccagc gtcaataccg ccagcgcaac
     2941 agctcccagc cgtgtccgca gatgctcgtt catcgcgctc cacccaaacc cctgcgtgca
     3001 ttgtacgcca gcacgctagg ttccagccaa tcatagggta gggtcccaac aaccggcttt
     3061 atcacttgag tttagagaaa agcaggacag acaaaaaccg tctgatacac cttatggaag
     3121 agggcttcct attttggtag gcgaacgtct tagaactgga cgctcaatcc gccccggaaa
     3181 taccgatatg acggaatcaa aaccacatag ccgtcccgtg tagccactgg gacatacccc
     3241 tgttcgagca ggttaccgac gttggcctct agctgcatgt gccctgggaa gacggccggc
     3301 aacggctgcc gaatctcgaa gctcaagtag gggtccatgt ggtagagcga ttcgccgtac
     3361 ggatcttgct gagagacggc aggaccatcc agccatttat agctcgcgga aatctgagtt
     3421 ccaaaatggc gcgacttccc cgaaattccc gcggccaggc tttgccggta gcgtgttgcc
     3481 aattcatcgc gcaaagccaa gccatcggct ccgccactcg gcgccagagc cccggcatag
     3541 gaatacacca atgtggtctt caggtttttg ggaagagtct cgcgatatac cacgcggcca
     3601 cccattgaac ccgtcgaacc tccgtcgtac gcaaacgatt cggataaata cgcctgcaaa
     3661 aagtcgggat tggaaacgcc gccgcgtcca atcacagcgg tgtgggtgga gcgatcatga
     3721 aaaaaggatg cagtgatgtc ggctcgcctg ctcagggaat gctccaccgc aatttcctga
     3781 tgactaccgt tctccaacac gggccggcca tcatgaagca tcaaactcgg aaaggcatcg
     3841 agtgtgttga gggtagagtc aacggccccg tcaccggaag ccgggtcggc ccaaggatcg
     3901 gtagccagaa taatcgatgc ccgccatgtc ttggcgactt gaacggccac ctcgccgtga
     3961 ggacgcacgg ccgccgtcgt tccgttgaag cccgccacaa taaatttcgc gccgtatcgg
     4021 acgctgacgc gatcccccag cgccatctga ttgtcctgcg tcaatcgcag tccacgaaac
     4081 acggaaccgt tgggcccaat ccgcgattca cggaccaaaa atgtggtcaa cggacccgcg
     4141 cccgccggac cattcggccg ccactcgcca gccaatccgc tggaagtgtc gacgccgctg
     4201 tagctgaatt gccccgccac cactagatcg ccctgctccc ctaggcccag gtcatagaca
     4261 accgctgttg ctggcgcgtt cgccaggttc gcaatggaac cggcgccatc cgcgccggac
     4321 gtcaattcaa cccgcccgtg ccgcagcggc tcgtcgctcg gcgtgtcagc tggctccgtc
     4381 gatgcagcta ccgccgtatc atcctcgtcc tgccagcgca gcacagggcg tgtggccgcc
     4441 gcgcttcgca gcacccaagt ccagtcgtcg gaggaaactg tgtcatccgg ttggcgtcgc
     4501 agtttctcaa aagaggagaa taccgaatcg accacgattt ccagaagcgt ggcttgttct
     4561 ccgttcactc gaacgtgctg ctccaccgcc ggcagaaatc cggccaaggt tacatgaatc
     4621 gtgtacattc ccaccggaag tgagggagta gcgaagtggc ccaaatcgtt ggttagaagc
     4681 cgcagcggta cgctcgctgc gattttttcg gaagagatca aaacggcggc gcccatctgc
     4741 ggcgtgccgg tgccatccac taccactccg gaaattgcgc ccgtattcgg ggcggctgcc
     4801 aaaggcgacg cccatgcaca caccaaccat ccgaggctca gcatgcagat tgcccggaga
     4861 gtcaaatggc tcacctcccc tgtgcagtga ccgccgcagc cgcaggcgcg gcttttaccg
     4921 tgaaatccgc tgtcggcgta atcgtttgct tggtgaggtt atcggtcacc tctacctcga
     4981 gatgatattt tccgggagcc agcgatccca gggtaatcag gttctcgagc gtcatttctt
     5041 cgccgtgctg cggaagttta tccgcgggga cgtcgaactt gagaaccggg gccgtgccct
     5101 tgccttgcat cactcgatac tccaccgatg tatccggctt gtgggttttg tcgtccacct
     5161 tcaggttgta cacttgaaga tatacgccca tggggtcttt ctgcgtaaac gttccatcca
     5221 tccgcggccg gaccttcaca tcgcccagaa caaattggcc aagcccgatg tccttgctcg
     5281 atacgcgctg gatgtcatcc gccagaatca gtgagctggc ggaaagctgg tcatcctgga
     5341 agcgtggaac gggaagcctt gtgttcacca ctcccacatt gccgctgttc acgtccttta
     5401 tcacaatgtc cagccggtag agaccggggc tgagcggcac ggccttctgg taaattctcg
     5461 atgtgcttag tgacttctgc agttgccctg aggggaagtc cgcccgaagc gtatcctcga
     5521 atgtctgcac tatccgtccg ctcaggctgg ttacccgcgc gaacagatcc atggtggccg
     5581 aatccactcc gcctttttct tgaaagctca attgccgcac cggaatctga actgtgatcg
     5641 gcacaagcac tgtatctgac gtgatgcgga cgaaatcata ccggtaatcg aacttcactt
     5701 gatcgcgcac caaccggttc gtaaccaccg cctccagatc tttaaacttc accggcggcg
     5761 gcttcatgat gttcgcgtaa ttttccagcc gcgtgaattc gttgtctttc tcgctcaacc
     5821 cgcccaattg cccttccgcc atgttggtgc cgtctgtgtt gttgaaacga gaattcttgc
     5881 tcgccattcc catggattcc gccagcgtga ggccggcacc cggcacgcgc agcagcgcgt
     5941 ctttttcgct gggatccatt gtcaggtgat actcgcccgt cgacgtcggg tccacgaact
     6001 ccaactctac gttttcgccc atgccttcca gataccggta acgccagtct tcgtatgggt
     6061 acgtcgtcgt cgaaccgccg ccctggtcca tcggccgatc ccactgtccc ccggtcggat
     6121 gcgaatcgac ctcatctggt ggcccccaca tgatgtagat cttgccgcgg tccgttttcc
     6181 agcccggaat gcccgatgcg tagtgctcgt tggcataggc aatccgccga tagtgttcct
     6241 ctttgaaagt gttctccggc gaatccggat cgggatttcg gcgctgccag aaggcctcga
     6301 taaactgctc ccgttcctca ttagtctgca gttgcaaaaa ggaacgccgc tcttcaggag
     6361 aaatgatgta aaccacatcc tcgttaagcc atttcttata ttgggagttt aattctttgt
     6421 agaggtcctt cagactcttc tgcttggctt tggaggactt gccgtggttt tgatctgcct
     6481 gctggtcttg ctgctgatcc tgattatcct gctgcgctgc tttggcgagg tgaattggta
     6541 tagtgctgac gcatgccgcc agaaaaagtc cgaggagcgt cttcgcaggc tggcccataa
     6601 ttgccccttt tccgattgcg aaatcttatc ctgtaccaat gtacaggtca attaaagttt
     6661 acccgtggtc ccgtgcgcca gagtcaggct caaactattc tcttttgccc gaggcgaagc
     6721 ggaattatga tggttggcat ccggctaatg ttgcttcgtg agctgtggca tggactgcag
     6781 gaagtccgtt gggaacgtga aataggttag actatccaga attcggagat ttgggttcag
     6841 tttgccgctt tttgcgcctc gtccgctggc cgcgaaacat tatgtctcgc tcgggcgtat
     6901 ctatcaagtg gcgagtatgt gtgtcgaaac ccttgcccag aggataagta atgaagacct
     6961 ggcagaaggc gacgattgca gcttgtgtgg tagtgctgat cggcggtatc gcctggtaca
     7021 gcatttataa atctaatcaa ggtgtcaccg ccgtccaaac cgcgcgagcc gcgcgcgagg
     7081 atctcgtatc gctcgtcact gcctcgggcg aaatccggcc gaaaaactac accaacgtcc
     7141 tcggtgaggg catcggcaag attacggaca tcgccgtcaa ggaaggcgat cacgtcaaaa
     7201 agggcgacat tctcctgcac ctcgaaaacg ttcagcccgg cgccgacgtg caagctcagg
     7261 aagacagcat taacgctacg gaggccggtc ttaaggcggc ttcagcaagt tacgatgtcg
     7321 ctgtcgccac gcttgctcag cgtcaggccg atctcgacaa ggccaaattg gattggcagc
     7381 gcggccagga actctacagg ggcaaactca tcgccaggca ggagtacgac tcggacaagg
     7441 ctgcttacga tagcgcggtt gcgtcggtta actccagtca ggcgcaggtg gaccaagcac
     7501 gcgcggcacg cgagcaggcg cgcgccacgc tcgaccaaac ccaggcagtc ctccgccgca
     7561 cccaggacat cctgcgtaag acaacctatc gggctccgat cgacggcatc gtcagttaca
     7621 ttgccgtcag ggtcggtgaa aacgtcgttc caggcattca agggaccgtc ggcagctcca
     7681 tcatgaccat ctctgacatg agcgtggtca ccgctgaggt caaagtagat gagaccgata
     7741 tagtcagcgt gaaggatggt cagcccgctg acgtcagcat cgatgccata cccgggaaaa
     7801 ctttcagggg ccatgttacc gaagtgggcg aactggcgat cctgcgcaca tcaggcaacg
     7861 cttcgatgac ggaaaccact gcgaatacgc aggaggcgcg cgatttcaaa gttgtaatca
     7921 ctctcgataa tccgcctaat acgcttcggc ccggactctc gctcaccgcg aaaatccaaa
     7981 cggcgcagaa gcaaaatgtt ctcaccgtgc cgattcaagc gctcgcggag cgatcacaaa
     8041 aagaattgga cgaagcaaaa aacggaaata agggcagcgt taccttggcg gcttcgacca
     8101 gcgaaggcgc gtccagcgtg aaggccgata ttcaaggagt tttcgttctc cgcaacggca
     8161 aagccgaatt cgtgcccgtg cagacgggaa tcaccggtgt caccgatatc gaagtcacca
     8221 gcggtttgca cgaaggcgat ttgatcgtca ccggcagcta caaagccatt cgcacgctga
     8281 agcccggcgc gcccgtcaag atagacaatt cagtttccca gaccgacact tcgtccagct
     8341 agaatggtcg cttccaggag tcctatgccg atggccgtcg aagaagtcgg ttcgcaaagt
     8401 ttccgccagg acttggtcga ttccggcatc gttatccaca ccgaagacct ctggaaaacg
     8461 tatgaaatgg gcacggagca cttgcatgcc ttgcgcggcg tcagcatcac catccataaa
     8521 ggcgagtacg tcgcgatcat gggtccttcc ggctccggca agtccacgtt gatgaacttg
     8581 atcggctgtc tcgatacgcc cacgcgcggc aattactggc ttgcaggccg cctggtaagc
     8641 caactcgatg acgacgaact cgcctccatc cgcaatcagg aaatcggttt cgtctttcaa
     8701 acgttcaacc tcttggcccg cgccactgca ctccacaacg tcgagctgcc catgatttat
     8761 aacggtactc cttccgagga gcgcctcgat cgcgcgaaaa aagcgctcgc gcaggtggac
     8821 ctcagcgaac gcatgatgca caggcccaac gagctttccg gcggacagcg ccagcgtgta
     8881 gccgtcgccc gcgcgttggt caataaccct tccatcctgc ttgccgacga gcctaccgga
     8941 aatctcgatt ccgccacggg cgcagaaatc atggcgctgt tcgctcgcct tcatcaaacg
     9001 ggcaacacta tcattctcgt tacccacgaa gccgacattg ctgagcacgc tcatcgtgtc
     9061 atccgcgtcc gcgacggcaa agtcgaaaga gacgaaccca ccccgcacct tcctcccgac
     9121 tgacgccatc tcgggaacgc cgtttcggcc gtcagatctc cgtgtcacca tcctgttcac
     9181 gttggtcagg tttctgaaag gcacgccagc ggtgattatc taccattccg ccaatcatga
     9241 aggtgtgtct ccccatcgtt gcgacatttt tggggaattg gagtgacgac atggaacgac
     9301 ccgcgttttc tgcttcaatc cattcagcct cggaacgcaa tcttctaggg ttctcagtgc
     9361 tatccggtgt tgaaaaaaat gtacgagatc cgcgcgcaat ttgcgcgcgg cgaatcctgg
     9421 cgcaagtttc ctcgctctta gtggctgttc tctttctcat aggggttctg agtgtgacaa
     9481 gactgggagc tcaagttgcg aataccgcaa caatccaggg cacggtaacc gatgcttcag
     9541 gtggcgtcct gcccaacgct caagtgcagg taaccaatac cggcacaggt ctcgtgcgaa
     9601 cgacgaccgc ggacgctcaa ggtcgttaca gcgttctcac gctgcaggtg ggtacgtatg
     9661 acgttcaagc ccttgctacg ggcttccaaa aagttactca cacaggggtc ctccttactg
     9721 ttggcaacat ccaaaccgtg gatttttcgc tgccagtcgg cacggcgcag gaaaccgtta
     9781 ccgtggaagg taccgcgaca caagtacaaa cttccgacac atcgattgcc cacctggttg
     9841 aaccaactca gatgacgcaa ctgccattga acggccggaa cttctcatcg ctattgctcc
     9901 ttgagcccgg aatacaatca acgcagtcgg gttcttcttt ttatggaacg cagaataact
     9961 atacgattgc cggccaaagg ccccaaggac aggggttcct gttggatgga tctgatgtct
    10021 ccaccttctg ggatcacggc accgggtcgg gtgccaccgg gaattcactc ggcatcgatt
    10081 cgatcgctga gttccaagca ttgacgaaca cctacagcac tcagtttggt ggcagcggcg
    10141 ccgtggtgaa cgccacgacc aagtccggaa caaacggttt tcacgggacg gcctacgagt
    10201 ttttgcggaa tagtgcgctc gatgctcgca acttcttcga cccaccgaaa atcccggagt
    10261 tccgccggaa ccaatttggt ggaagcctgg gcggaccggt cgttaaagat aaagccttct
    10321 tttttgtcaa ctatgaggga ctccgtcaat cgctaggcca caccgaagtc gccaatgccc
    10381 ccgatctcgc tgcccaccag ggctatgtcc cgaactcgat gggagtcttg gttggagcta
    10441 tgccggggtg tccagcttac tcacccgcgc tgccgatgaa tacgtgcgtt gctgcgaacg
    10501 tccagtcgac actagcgcta tttcctgttg cccagaacga cagcgtggca ccgggcgtcg
    10561 gaaccgtggc ggaggtcgcc agcgacgtcg cgaacgaaaa ctatctcgtc gcccgtttcg
    10621 attacacctt gtcggaaaaa gactcgttct tcctccgcta catccgtgac acagcaagcc
    10681 agactctgcc gtttagcggc tcgccaattc cgctctggcc ggaaggagac accactgcca
    10741 accacttcgc tacaatcgga gagcggcaca tcttttcgaa taaccttatc aactcggttc
    10801 actttgaatt cgatcgaccc aaagatgtag ggaatacaac cagcagccag gctgcgacca
    10861 agtatttcga tggacgcgaa gacggcaact gttgtggcgg cttcggtggc agcttccctc
    10921 tgagcccgat cggagcgaac gctttactac cgttcagtct ccctcaggac aagtttgttt
    10981 ttgcagatga tatcctatgg acccacggct cccacagctt cgcgatcggc gctgccgtga
    11041 aaagggaata cacctatacg tttgcacctt tcgagtgggg cggttcttgg acgttcgcga
    11101 atctccaggc ctttctgggt ggccaggcta gctcgctctt ctccgcggcg gcaggtaatg
    11161 tgatttccaa caaagatttc cgcgaacttt tcgtctcgcc atatttcaac gatacatgga
    11221 aagtatcgtc gaagctgacc gtcaattatg gagtccgctg ggattgggag accgatccga
    11281 ttcaaacggt ctacggcatg caggccttcc cgaatccacc ggtgggttca tcgccaggct
    11341 gcctgggcgg cggatgcttt gagtcggtca ctcatgcctt cgcctcaaac tttaattaca
    11401 agaactggga acctcgtgtc ggcattgcct acgatccatt cggggaccac aagacttcga
    11461 ttcgcgcggg ttttggtctc ttcgatgacg tggtgatgcc acgcgaatat atgcctggtt
    11521 actggctcga cccgccgttt cagcttgcca ttcaaatcaa tccgccatac ccgaccccat
    11581 tcggctctgg tgggttcagc tttccgcttg ccccgattca accggaggga caagtctatg
    11641 cttggaagta cacaccgtat tcattgcagt acaacttgaa tgtgcaacgt gagctctttc
    11701 ggaacaccgt tctcacgatt gggtatgttg gctcgcaggg ggttcatcta acgaaagcta
    11761 acgacgtcaa cccacccggg atgatcgtga atggcaaggc ggtgccctac atcaataacg
    11821 gcacgcagtg gccagtccaa cggggaggag ggctttgcac gacacttccc cctccggatg
    11881 ggcagatagt ttttggctgc ctcgacgcca ccggtctttc cgtcgaccca ttgcagcagg
    11941 ccaatccgac tttgggtcaa ctcagtgaga gggagcccgt cgctcattcc cactacaatt
    12001 cgctgcaggc gagtgtcaac cgtcccttta cgcgtaatgt gcaaacacaa ctgtcctaca
    12061 catattcgaa gtgcatcgac aacggttcgg tgagttccgg tctcgaagct ttcaatttgg
    12121 ccgcgcagct cttcacgaac ccctacaatc tagcgctaga cgtgggtccc tgcgatttcg
    12181 atcagacgca tagtttccgg ggcagtgggg tggtgggcct gcccttccac gggaatctct
    12241 tcgtcgaagg ctggcagctc agcgggattc tctctgccaa cagcgggtat ccgatcaaca
    12301 tcagcactgg atacgatatt tcgggcgagg cgtctggtga ccgtcccaac ctggcttctg
    12361 gttccccacg ccccacttcc ctcgggcatc cagcccagtg gtgggccttc ccggcaaaca
    12421 tgacaatgcc tctggtcggc gaactgggta acgtgccaag gaacagcgca ctcggcccgg
    12481 gcttcttgaa tttcgacttt gccttactca aagacacgcc tgtaagaaaa atctcggaac
    12541 agtttcgtat cgaattccga gccgagttct tcgatatcat caaccatgcg aactttggta
    12601 ctccgaatgg aggactcttc atcgcctgtt caactacgga cccagcatgc actccggcct
    12661 ttgccggcgc caatggcggc ttcgagcgca gctcgacgtt tggcttggtt cagacgacag
    12721 ttggtaatgc ttccacaggc ggcagtgaac gcgtcatcca attcggactg aaattgctgt
    12781 tttgaggtcg ctggcgcacg cgacatggct gagccgttca gggtgaatca ctcatcttgt
    12841 gcgcgggtag tcttaggaaa gcccatgtcg ccggtaagcc gccggagata cacccacaag
    12901 ccgaagaaac caatcggaaa gatggctttg ggatgagaac ccggaacaaa gggcaatctc
    12961 agcgatgggc atatcgctgt tacctagaag ctttctagct cgctccaccc ggcgctgcag
    13021 cacgtattga tgcgtcgatt gcccgacagc gcttttaaag gcccgcggca ggtacagagg
    13081 gctgatgttc acctccgctg ccagcgcctc gaggctgaga tcgcggtcca aattctcctc
    13141 gataaaatcc atcaggcgtc gaattttcca cgtgggtgcc gcatttgcaa ttgccctgtt
    13201 gctcggtcct gagcgcgagg agtgataacg cgcaagctgc actgcaatca tctgcgcgag
    13261 cgtgtcgata taaagaccgt ctctcacgct gttatcgcgc aatcccgttg caatcgtcag
    13321 cgccagttgc tcgagaagcg gatccaaaat agcgaagcga ggcacaatct cggccgccga
    13381 tgcgtcgcag ccgtacatct catttacagc ggcgctgtaa atggactggc gcaggtaaat
    13441 ctgaagaatc tcaggatgac catggtgctg ccatcgagcg gcggtccctc cgggagtcac
    13501 gcagattcgg cgaggcccga taagcgccct ctccacgtgc gcccccggaa tcttgcgcgt
    13561 cacttccgtt ggatggtaca ggtgataaat cagaaaagga tggggaatgg gtgtttctac
    13621 cgcttccaaa ggaccttctt cgataatcgc cgcgtacaga gagcgccagc caacctctgg
    13681 gctgtgggcg attaggcgat tttcggcttt gagcgcgcgc ccatgcgtct cacgagcccc
    13741 aaatatttca ttcttcggcg taactgtgga actacgtggt ccgctagctg caatcatcgg
    13801 agcgatgccc acccaaatcg cgcaaatcct aatgcgccgc ttttcagccg tcaagccaaa
    13861 ttaatgcaag ccattcgctc ggcgcccgaa ccgagcgccg acttccggcg cccagcgcgt
    13921 acctttctcg ccgcttcaca ccgggcgagc cttccgtttc gtgtgattct cggcatcgta
    13981 cgatgcgagc cacaggtaca tgagggtaca atcccgcgcg acctagatgc gtcaaacaga
    14041 gacctaggcg ggcgtgccgt ggcttttgcg tcttgcatcc cagcggaata gaaaaaacaa
    14101 cgcgctggca atcagcagga tggttgcgcc gcccgcaatt tgcagcagtc ggcccaccag
    14161 agcgtggtat ctccccgtcc ccggatcgta cgtgcagcag tacaaaagaa tttgatcgac
    14221 gaccgagccg atttgttcgt gcgatccttg caccagcgcc agccgcaaat cttggaccgg
    14281 atactcgatc ccgtaaaaat attgagccac tttcccggcc ggcgtgagga ccatgattcc
    14341 cgcggcgtgc gcgtattgat tgagctgcgg aatatatttg tagtgaaagc cgacggcgtt
    14401 cgcgaggcta tcgatctgcg gttttttccc tgttagaaaa tgccagcccg ccgctgccga
    14461 cggctcgccg tattgtttta tgtacgtgcg cttcgtcgac gcggccagcg ctggcgtttc
    14521 ttgcgggtcg atgctcacgg tcaaaacgtt gaactgatcc ccgatctgga aattcaactt
    14581 tttgaacgcc gaagccgctc cgtgcaggac ttgactgcaa agcatagggc agcggtaata
    14641 cgccatgatg aggaccaccg gttttttttc aaaataagaa cgcaggacga ctgggtttcc
    14701 attttcatcc gtaaaagttg catcgagcgg aatctgtttg ccaagccgct gttcgaaata
    14761 aactcctttc aaaatatcgg gcagctgccc cggcgttgaa gcggagcccg ccgacagttg
    14821 cacgccgcca accgactgcg cctgcgacgg aacgccaacc atcatcgtgc aaaagataag
    14881 tgcgccgaca atcgcgaaac tgcgaaccca aaaataaagc ccttgcatat gaggccccct
    14941 aaaatgccgc gaaaaaaaaa ctgcgcttcg gaacgcgtgg gcactggcga gcaggaggaa
    15001 aataaaaacg cgcagcagct accatccgac gagagtcatc gagcgatctc gcggcgcaat
    15061 gggcgtctgc tacgaacgga ggcggacact acgtcaaaaa aaccaaacct cgccgtcccg
    15121 gaacccagga aaatccaaat ccgacgaagc gaggccgaaa atcgctgcga tcactcagat
    15181 ccatgaccgc catttaccgt cgaagagtgt agcaaagaac cgcgcaccaa atccgtacaa
    15241 aatcgggcgg cgtggagggc tgtacggagc ccaaaacgac cgcatattgt ttctagttag
    15301 aatgatttga tgctgcaaac tagaagtaaa ttcggtgaga aatagcaacc gaatggggct
    15361 gcgaaaagcg gtggcaaatc atccggaaaa gaggttgcga gcgaggaaaa agatgttggc
    15421 ggggcgctcg gcgaggcgcc gcatgaacca cgggaaccag aaagagccat acgatatcag
    15481 cacgtcgctt cggtagcctt tgcgcgccag gcggaactgc tccttccgct gaattccgta
    15541 gagcatcgcg aattccaatt gagatttttc gatgctttgc gccgatgccc attccgcgat
    15601 tcgagcaact aacttcgtat catgcgtggc tatcaccacg cgcacgccag tctcgcgtgc
    15661 gcgcgaactt aaaagatttt gcgcgagacg aaaatagttt tcatccacat ctttcttttt
    15721 cgcaaaggcg atttcgggcg gctcgctata cgctcctttc accaggcgga cggaagatcc
    15781 cagggcgatc agcgattcga catcttgttc agtgcgacgg agataggcct gcacgcatac
    15841 gccgatgttt gcatgtgtcc gccgcgctcg cgcgtgcaac tcgagagtcg cttccacgta
    15901 gcggctctgc tccatgtcaa tccagattgt tttgtgcgga gaatgttcgg cgagggccac
    15961 aagatttgcg aagcagaatt cggcgtcgat atccaatccg agttgcgtca gcttcaccga
    16021 aatttccgag gggaggccgc cctgctgaat gcgatccagg agatttagat attgttgaat
    16081 ttccgactga gcctccgccc gctcattcac attttcgccg agatgagtca agagcgatga
    16141 aatgccggcg cgcgaaaaac ggctggcggc cgccagcgca ttgcccatat tttctccggg
    16201 caaaaaacgc gtggcggcgc gccggacgaa gggaagacgc ggcattgttt ttcgcagcca
    16261 cgcattttca gacgccgcta tcaggatttg gcgcgcccat ttcatgtgaa tttcacccgc
    16321 cccggctgag agcctcgctc cgattcgctg gccagaatag catcttcgtt gtgctcgcgg
    16381 gccaacgccg atagaatgct agtgtttcgc gatacagtcc gcttcccgca cgtggcaatg
    16441 ccgccgcggt cggcgctcac ctcagcccgg gaattgcgat ggcctccatg gacgcgcgtc
    16501 tctcaattga ttcgatcgtt tccgccacgt ccgagcagct ttcctgcgcg ctcggggagg
    16561 agtcggcgat cctgaatctg aaaaacaccg tgtattatgg cctcgatcca gtgggcacgc
    16621 gcatatggaa tctgctccag cagccgattg gcgtccgcga attgagagac cgaataacgg
    16681 atgagtacga agtcgatgca aagcaatgcg aaggcgactt acttgaactg ctgacgacga
    16741 tgcaaaatga agggctcatc cgggtggaag gttcagaccg aggctaagaa acggcgcggc
    16801 ggtgatcgtc cgtgaacagc tggcgtgcat tttggcattt gagccgggac atgcgaggtt
    16861 tcgctctgga agccgcgagc gtgctgacaa tcacctggct gggattgcga agcctgggtt
    16921 tccggcgctg gcaaagactg cttacgcgag tgatgcccgc cgctccgaac acctttgaag
    16981 cgagcgaatc cctgctggac cgatgccgcg cgattgcgcg aatcgagcag tccgtggccc
    17041 gccgtctccc ttttcaatcc aattgcctcg atcaatctct ggtgctctgg tggatgctac
    17101 gcagacgcgg gatggccgcc gagatgcata tcggcgggcg aaaagacgcg gcgaatttcg
    17161 aggcgcacgc ctgggtcgaa gttgggagtt atacatttcc cgagatgagc ggagggcatc
    17221 ggcccttcac gccatttgat ggcccaatcg aatctgtcga gacgcaaact cgatgagcgg
    17281 tatcgtcgga attcttcatc ttgatggcgc tcccgttgac gctgaactgc ttcagcggtt
    17341 ggtcgatttt cagaaatttc gtggcccgga cgcgcaacag atttggatca gcggccctat
    17401 cggtttgggg cacacgctgc tcaagacaaa cgacgagccg gtccacgaat gccagccatt
    17461 tcatttgggt gacgacattt ggattgcggc ggacgcgcgc gtggacggtc gatcggatct
    17521 cgcagccaag ctgaccgccg caggccagaa gcatcccccc gacgctactg atgcggagct
    17581 catactgcgc tcctaccgag cgtggggcga ggcatgcctg gagcatctcc tcggagattt
    17641 tgccttcgcg atttgggatg ggcgggagca aaagctattt tgcgcgcgcg atcaaatggg
    17701 tgtgaagccg ttctactacg caaacatcgg ctctctgctc gtattcagca acacgctcga
    17761 ttgtgtccgc caacatccgg cggtatccga tcgcttgaac gatctggcca tcgcagattt
    17821 tctgctcttc gaaaccaatc aggatgccgc aaccacagcg tttgccgaca tccggcgcct
    17881 gccggccgcc cactcattgc ggtgcgaccg cggagctgtc tctatccgcc gctattggac
    17941 tctttccgtc acggagcaga ttcggttcaa gcaggacgcc gagtgcatcg agcgcttccg
    18001 tgtgctgttg gatcaagctg tcgcagaccg tcttcgcacg aaaagcgctg gcattttgat
    18061 gagtggcggc ttggattcga caacggtagc ggcgacagcc cggcgaatcc tgactgcaag
    18121 cggcaacgat aatggattgt ttgccgagac cgtggttctc gacgaattga ctcccgataa
    18181 cgagcgccat tacgccacgc ttgcggcaaa tgctctgaaa atccccattc gatttcagac
    18241 ggcagatcat tgtgggcttt tcaaaggcgc cgaaaacccg gagtaccgtc cgccgtttcc
    18301 tgaacactca gcttggcctg accagactcg cgatctgctt cgcgaaatcg gcgcgaggag
    18361 tcgaattgca ctcacaggca gtggcagtga tcccggtctc tcaagtcgga tcacggtcca
    18421 tttcaggcaa ctcctgcgaa agaggcgtta cgttcgcgca gcgagtgacg cggtgcgcta
    18481 cctgagcgct gaaggccgct tctcgaggct ttaccttcgc gcgcgttggc gattattggt
    18541 tagctcaacg aacgcattca gctcgtaccc gaattggcta aatgaggact ttcaacgaga
    18601 atggaatctc gaggagcggt ggaaagctta cggagtatcg gaccgcgcga cgcaactgcg
    18661 caccgaaagt aacgtcaatg cgtttcgacc tgaggcgctg acgtcaatga tacatgtcag
    18721 ctggcaggac ctgtttgaga cgctcgatcc gagtgctacc cgtgttccaa tcgaagtccg
    18781 gcatccattt ttcgatttgc gagtggtcac tttccttctc ggcctgcccc ggttaccctg
    18841 gtgttgcgat aaagagctac tacggcaagc ggggcgcggc attttgcccg aagccgtgcg
    18901 aatccggcgc aagtcgccgg tcgaaaacga cccaatcgtc gccctcctgc aaaaatcaga
    18961 atcggcgtgg gtagatcgat tcgagccggt tccagaacta gggcgctaca tctgccaaca
    19021 caggattccc ccagtgtgga tggaaaaatc ctctggaaca gcctggataa acctcaaacc
    19081 tctcagcctg aatttctggt tgcggaccgg caagaattga ttcagtatca aatctggcgg
    19141 aggtcacgcg gtgaaatcag agaccaagtc acgtcagcca atcgactctc ccaagaagag
    19201 ttatcaaaag ccaactctca ccgtttatgg caacatagag aaaattacct tgcagggcac
    19261 cacctccacg gctggtgaca atcgggtcaa ggcgaaccat aagactggcg ggtgatttct
    19321 ctttgtcgcg tcaaggcatc tttagaattg gagtagcctc tgtggcgctc atgccggtga
    19381 tgcagaggta agctcgttcc taatgcagag tgtggtcctg tgcgccaaaa ccagaacttg
    19441 cgccgatcct gcgcgaattg aaaaaggcgc ttgacctaac gagttcacaa ccacggtccg
    19501 tccatggctt ttcacagact ctacggcctt cttctcgaaa cgaatatcgc catacccggt
    19561 gtccctgtct tgccctatgc ggacggcatt atcgacgtcc aaatccgact taaatccgag
    19621 aggtcaccgc tgtggagttc gaagttgccg tcagaagcct tctacgtgag ccggatgagg
    19681 gacgcgagcg ggaatcccgt tttgcgagct ggcttattgg gcgatgctag acacttgtcc
    19741 ctcgtttata gcgacggaac acattttttg ctcgaagatc gagggaagga gatctttgcc
    19801 gactggcctg atccgttaac gcttgaagac gcttccctat acctggtggg gccgattctg
    19861 ggagtcgtcc tccggctgcg gggcatggtt cctttgcacg ccagtgcggt ctcaattggc
    19921 gatcaagcgg tcgcgttggt tgggcctgcg ggagcgggaa agtccaccac tgcagcagct
    19981 tttgccagat gtggttatcg cgtaatttcc gacgacgtgg tcgccctggg acaagaagca
    20041 tctcgttttg cgataccgcc aggctatccc cgagtgaacc tgtgggttga gtcggtgaaa
    20101 gctatgttcg ggcccggcgc ctcgctgccg cccatctgtc ccacctggga caaacacttc
    20161 atgccgctcg atccgcacaa ggaattcgaa gcgaggtctt tgcctttggg cggtatctat
    20221 gtcttgcaaa aacgcgcggc gggaatcact gggccgatcg tggagcaact gacgggcaca
    20281 gaagcgttcg tcgccctcct tgggaatacc tatatgaact atctgccgga tcccgataag
    20341 cgctgtcgag agtttgagct gctgggacgc gtggtaggcg aagttccaat tcgcaggatc
    20401 caggcagccg ccgatctttc aatgctaccc gaactatgtg cgacgatagc ggccgacatg
    20461 gagaatctgc tggcggcccg ccagctcgag aaggctccga gcagcgcctt gcaagctggg
    20521 ccttgttgat ccccacaaac ctctcggctt gtcggcgcta agtttcgctc gaaattgcac
    20581 tggtgccgct gggcgctcgg tgtctttgcg aagcgatctg tattgcgagg ctcccggaag
    20641 ctgcaaatat cctacatgga tcttaggcgg gttagcagcc tagtaacagt accataattg
    20701 catgagcggt cgcaggttag cggcaacaca cggaggcaga aaactgccta gaatatgaaa
    20761 aactttgttc cgaaacaata caggcggatg aacccttcat ttatgggtgt cggggcgttg
    20821 cgacccgatc gaggttataa gcgagacgta tcctgtatta cgttcaatgc gttacgcaat
    20881 aggccgcggc cttggggaag ggtccgattg cagcttcgcg acgcacaggt tttggctggc
    20941 aacgtgcctc tcatccagca gactgcgagt tgcaggctgg gagagtctat gacgcgtcga
    21001 ttgctgtcta tttgcttttt catcgctatc tttgggcttt tttccgggag ccgcgctaag
    21061 gcggacgatc tgtttacgta ccaattcggt ggtaacacgt ttacctggga gttgccggcc
    21121 tcaccgaccg ttgtcattcc ccctgatatg gcgaccgctg gcatgttttt tgagctggtg
    21181 ggcgtaccgt tctccgaaaa cggcggggcg cagacccagg gcacgcttga cttctttcaa
    21241 gcccccgaca acggcggcgg attccagctt ttcccgatgg gtaactcggc cgcaattgtg
    21301 aacgcgtttg gcccgcagct ctataacggc ggagaaggtt cgcccacatt taagttagga
    21361 acattcgcac taaacaatgg tggtgctgac ggtcccctgg gtactctgac aatcaatagt
    21421 tttagcacgc ctgagcccag cagcttgcta ttgcttggcg ccggggcgct tgctttgctc
    21481 ggtttaacgc gcaagaggat attcgcccat cagatgtaat cgattgccca gcgggcgagt
    21541 ccaacgagtg gtctctccac tcgccttgtg aattcctacg gcaccaacct gctggacagt
    21601 gaaatgcggg cgcgcgaaat tcgagcctgc tgggggccgc ttggtcgctc ccgttccatt
    21661 tggaatatcc ggccgcaacg ccgacccctt cgcggatcca tcgcttggcg acctgatctg
    21721 gtccacgatt gagttcgcgc ttccatctct gcccagaatt tgagacttca aaattcgacg
    21781 cagcaaagat caaccgtagc tttcttctga agataagttc gcgcggcaaa ccaaccacat
    21841 cggggtatca tgccttccaa gcatccacgg ttcgcttacg cattttagga gaaggtttcg
    21901 atgcagatga cgaagctggc gggaatttgc gcgctgttgg cggttttcgc ggcgacaacg
    21961 gcgatagcgc aggatcaaac cacatcggat cagacggagc cagcttcaac gccgagtgcg
    22021 ccgcaagcgc ccaagaaggt gaagcgagtt tacacggcgt ggaaatacga ggcttccgcg
    22081 ggattcgcgc atcggtcgta ctacgcgccc ggcggaggaa ctttcggatt ggttggctgg
    22141 tatgcgtccg gcgaatacaa ctggaagaag tggcttggcg cggaagggga agtgacagat
    22201 acctggtaca cgctgaagag ccctgggttt cctcgtgaca cgatttacat ttacacattt
    22261 ctcgccggcc cgcggttcta tcctttgggg caccacaagg taacgccgtt cgggcacgtg
    22321 ctcatcgggg cggggcacta tagcagcgct actccggcgt tcggcggttt tcacggcgcg
    22381 acgctcacgt ccaacgcata cacctacgaa gttgggggcg gactcgattt gaatctttgg
    22441 gagcactggg gagtccggat gattcaggcc gattttggca gtgcaaacta cgtgaacaat
    22501 cccaattata cgaatcgcgg gagtacacgg ttctcggtgg gattcgtgta tcacttcggc
    22561 gcgcggtaac tgcgccgcta aatttttgct gctcttttca ggagttcttc tttagttcgg
    22621 cgaggaccag cttggctact tccttgaggg tgtcgaagac gccctcgccg gtgatggcga
    22681 cagcttcgcg cacgggctcg cccttcacat tcagctgctt ctggagttca tcgacgggaa
    22741 gcacattggg cagatcgcgc ttgttgagct gaagaacata gggaatggtg gtgaaatcga
    22801 gattgtgatc tttcaggtgt tcgcggaggt tatcgagcgt ctcaaggttg gcgtccatcc
    22861 gctcttcctg ggaatcggcg acgaagacta cgccgtccgc gcccttcaga atcagacggc
    22921 ggctggcttc gtaaaagacc tgccccggga cggtatagag atggaagcgg gttttgaagc
    22981 cgcgaaccgt acccaggtcg agcggaagaa aatcgaagaa aagcgtgcga tcggtttcgg
    23041 tagcaaggga gaccattttg cctttttgcg aagtgcccgt atggtcgtag acccactgca
    23101 agttggcggt tttcccgcca agtccgggac cgtagtacac aagcttgcag ttaatctccc
    23161 gagcgggaaa gttaataaat ggcacgattt cctcggaaag caggcagcac tattctttat
    23221 agcatacgcc ctatgtgcgg caaaacgcgc ctgataggcc cgtgcttcgc aactgacaac
    23281 tcaatgccgc cggacggtgc cgggaccaag ggtagaagat tgagaaacag cacgcgtggt
    23341 tgggagggcc tttgcgcctt ttcggattta caatataggc cgctccacca ctagaaccag
    23401 acccgggcct cccagccaac agagattacc tcgggcctcg atgatcacag ggcgactact
    23461 agctggtgtg tccgatgagc aaaatctgaa tacgaaagag aagcgaatca tgccgatacc
    23521 atacagagcc gatcatggcg gaagttttct gcggcctatc gaattgctgg aagcgcgggc
    23581 caacggaact gacccgggcc gtctgcgcgc cctggaagat ca
//
LOCUS       DATNDT010000104         4317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3535973, whole genome shotgun sequence.
ACCESSION   DATNDT010000104 DATNDT010000000
VERSION     DATNDT010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3535973"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VN788_03250"
     CDS             <1..496
                     /locus_tag="VN788_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU19582.1"
                     /translation="FNDTVRNNLCYGRPDMPDDKIIAAAQAALAHDFIIRMPQGYQTV
                     IGDRGQRLSGGQRQRLAIARALLKDAPILILDEATSELDSESEMLVQKALNNLMTGRT
                     VFVIAHRLSTIRRADMIAVLDEGSIRERGTHEELLERGGSYARLYEMQFRDAETVTRA
                     AGPA"
     gene            649..1530
                     /locus_tag="VN788_03255"
     CDS             649..1530
                     /locus_tag="VN788_03255"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HXU19583.1"
                     /translation="MTGYGEARLSESGFNVRVAVRSVNHRFLDLHLRMPEGFEPIEPR
                     IRKIVRQRLRRGHLDVTVRHELVGPSAVGVNQDVAAAYLDAVDALRKRFDISTRPDVA
                     AILRLPGVIGPAAALLDGDLPRLEELVAKCLAAAIDKLDRMREEEGAALLTEMSDHLS
                     RITSLTGKVETLAERARPAFAKRLQMRLKELLDEAPLDPARLAQEAALAAQRSDVSEE
                     IARLASHIGQFQTLLSSAVDVGKKLDFLLQEMQRETNTLLSKTPGNDAEGLEITALAL
                     EMKSEIEKIREQVQNVE"
     gene            1527..2147
                     /gene="gmk"
                     /locus_tag="VN788_03260"
     CDS             1527..2147
                     /gene="gmk"
                     /locus_tag="VN788_03260"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HXU19584.1"
                     /translation="MSESEPLVLIVSGPSGSGKSTLVGKILELPGTMLSVSCTTRPPR
                     KTESDGKWYNFVSESEFQRMVESGEFLEYAQVFGKNWYGTPRKSLDEALALKRDLVLE
                     IDVQGALQAKRELPNAVAVFVLPPSAADLERRIRERGQDSEDEIRRRLERARQEMLNY
                     RSYDFAIINDDLDRAGREVQSIALGSRCRIARKEPRIREILKSFGG"
     gene            2155..2379
                     /gene="rpoZ"
                     /locus_tag="VN788_03265"
     CDS             2155..2379
                     /gene="rpoZ"
                     /locus_tag="VN788_03265"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005548260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HXU19585.1"
                     /translation="MAALRQVPDSQFAYVVVVSRRARQLMIGGRPMVDNPKSRKTSRI
                     AEEELLQGLLEYELPELPEGSEESDAKRRR"
     gene            2395..3609
                     /gene="coaBC"
                     /locus_tag="VN788_03270"
     CDS             2395..3609
                     /gene="coaBC"
                     /locus_tag="VN788_03270"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HXU19586.1"
                     /translation="MKIALGVAGGVAAYKAAELVRRLQQEKLDIQVVMTRAAREFITP
                     LTFAALTGKKVITEMFGADDASPANVESAIEHIAVAQRIDLLLVAPATADILGQFAHG
                     IAGNFLSTLYLATKAPVVVAPAMNVNMWEHPATQENLNVLRARGVHVVDPDEGYLACG
                     MTGAGRLAATETIARRVCAVLGLRHDFEGQTVLVTAGPTREDIDPVRFLTNRSSGKMG
                     YALADAAARRGAHVILISGPTGLPIPESTDWVPVRSTDEMRNAVNERSREAEILIMAA
                     AVSDYRPIATRTQKLKRGDSRLILELEPTPDILAELGAEPLRRESKRILVGFAAETSS
                     IAENARAKLARKGADMIVANDVSQEGAGFDTDTNIVTLFLRDGREVSLPKLSKFDVAN
                     QVLDHVLQLQGK"
     gene            complement(3685..>4317)
                     /locus_tag="VN788_03275"
     CDS             complement(3685..>4317)
                     /locus_tag="VN788_03275"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family substrate-binding domain-containing
                     protein"
                     /protein_id="HXU19587.1"
                     /translation="CDSIFSTLQNAALAEGKIDIGFLRPPVNTVRLNSEFLYEERLIA
                     MMSRANLLAKRKTLRIKELAAETFFLPDASVGGGLRERTLELFAKAGISPRVSAMAAN
                     PLSHGEVHKVLLAANKGIFIIADEASTRLDNGNVAVAVPLDDPDAKIDVYMAWRKNEK
                     SPAVLAVLDTARSVLGTSAETTAVAASGVGLTHNRAGMYSEKTLVPRLQR"
BASE COUNT          945 a         1260 c         1226 g          886 t
ORIGIN      
        1 cttcaatgac accgtacgga acaatctctg ttatggtcgc ccagatatgc ctgatgacaa
       61 aatcatcgct gcagctcagg cggctctggc ccacgatttc atcatccgca tgccccaggg
      121 ctaccaaacg gtgattggtg atcgcgggca gcgcctgtct ggcggccaac gccagcgcct
      181 ggccatcgcc agggctttgc tcaaagacgc accgattctg atcttggacg aagccacgtc
      241 cgagctcgat tcggaatctg agatgctcgt tcaaaaggcg ctcaacaatt taatgactgg
      301 ccgcactgtg tttgtaatcg cgcatcgcct ttccacgatt cgccgggcgg atatgattgc
      361 agtgctcgac gagggctcga ttcgcgagcg cggcacgcac gaagaactcc tggagcgcgg
      421 cggaagttat gcgcgcctgt acgagatgca gtttcgcgat gccgaaacag ttacacgtgc
      481 ggctggtccg gcataaaggg gtcgcggggg ccgagtttaa tcttagctgg agcttttttt
      541 acaagaaacg cgaagtttgg tcggcgagcg aaaaacatgt ccacaaaatc acagcgagca
      601 cccgttgctc agctagactc ccgagcgctg cctttgagtt taaaatcgat gaccggctac
      661 ggggaagctc gcctcagcga gagcggcttt aacgtgcgcg tggccgtccg cagcgtcaac
      721 catcgctttc tcgacctgca tctccgcatg ccggaaggtt tcgagcccat cgaacctcgc
      781 atacgcaaaa ttgtccggca gcgcctgcgc agggggcacc tcgacgtcac cgttcgtcat
      841 gaactggtgg ggccatctgc tgttggcgtg aatcaggacg tcgccgcggc gtatctcgat
      901 gccgtggacg cgttgcgaaa gcgattcgac atatcgacgc gcccggacgt cgccgcaatc
      961 ctgcgtttgc ccggagtgat cggcccggca gcggcgctgc tggatggaga cttgccccgt
     1021 cttgaggagt tggttgcaaa gtgtctcgcc gcggcgatcg ataagcttga tcgcatgcgg
     1081 gaagaagaag gggccgccct actcaccgag atgtccgatc acctttcccg cataacaagc
     1141 cttacgggaa aggttgagac cctggctgaa cgtgctcgcc cggcgtttgc caagcgcctt
     1201 cagatgcgac tcaaagagct tctcgacgaa gcgcccctcg accctgcgcg cttggcacag
     1261 gaagccgccc tcgccgccca gcgcagcgat gtctcagagg aaatcgcccg cttggcgagc
     1321 cacataggtc agtttcagac gttgctttcc agtgcggtgg atgtcggcaa gaaactcgac
     1381 tttctgctgc aggaaatgca gcgcgagacc aatactctgc tgtcaaaaac tccaggtaac
     1441 gatgccgaag ggctcgaaat cacggctctt gcgctcgaga tgaagtccga gatcgaaaaa
     1501 attcgcgaac aggtgcagaa cgtagaatga gtgagtccga acccctggtt ctgatcgtct
     1561 ccggcccgtc cggatcggga aagtccacgc tcgttggcaa gattttggag cttcccggaa
     1621 cgatgctatc tgtttcgtgt accacgcgcc ctccgcgcaa gacggaaagc gatggcaaat
     1681 ggtacaattt cgtatctgaa agcgaattcc agcgaatggt tgaaagcggc gagtttctgg
     1741 aatacgctca ggtgttcggg aagaattggt atggaacgcc gcggaaatcg ctcgacgagg
     1801 cgcttgcact aaaaagagat ttggtcctcg aaatcgacgt gcagggagcg cttcaggcca
     1861 aaagagagtt gcccaacgcc gtcgcggttt tcgttctgcc gccgtctgcc gcggacctcg
     1921 aaaggcggat acgcgaacgc gggcaggatt cagaggacga aattcggcgc cgcctcgagc
     1981 gtgcacgtca ggaaatgctc aactatcgca gctatgattt cgctatcatc aacgacgatc
     2041 tcgatcgggc cggacgcgaa gttcagtcca tcgctctcgg ctcgcgctgc cgcattgccc
     2101 gaaaagaacc gcggattcgt gaaattctga agtccttcgg aggctgattt gctcatggct
     2161 gctctcagac aagtgccgga tagccagttt gcttatgtcg tggtcgtctc ccgccgcgca
     2221 cggcagttga tgatcggggg gcgcccgatg gtggataacc ctaagtcgcg caagacttcg
     2281 cgcatcgccg aagaagaatt gctacagggc ctcctcgaat acgagctgcc tgagctgccc
     2341 gagggatcgg aagaaagcga cgccaaacgc cgcagataat cggtcggttt ctccatgaaa
     2401 attgcactgg gtgtagccgg cggggtagca gcgtacaagg ccgccgaact ggttcgccgc
     2461 ctccagcagg aaaaactcga catccaggtc gtgatgacgc gtgccgcgcg cgagttcatc
     2521 acgccactca ccttcgccgc gctaacaggt aaaaaagtaa tcaccgaaat gttcggtgcg
     2581 gatgatgctt ctcccgccaa tgtggaaagc gccatcgaac acatcgccgt agcgcagcgc
     2641 atagacttgc ttcttgtcgc acccgccaca gccgacattc tcggacaatt cgcgcacggt
     2701 atcgctggga actttctttc gactctctat ctcgccacca aagctccagt tgtcgtcgcg
     2761 ccggcgatga atgtcaacat gtgggagcat cctgccacgc aggaaaattt gaacgtcctt
     2821 cgcgcgcgtg gcgttcacgt cgtcgatcct gacgaaggct acctcgcctg cggtatgacg
     2881 ggcgccggcc gtctcgccgc tacagaaacc atcgctcgca gggtctgtgc tgtgctggga
     2941 ctgcgccacg attttgaggg ccaaaccgtt ctcgtaactg ctggccccac tcgtgaagac
     3001 atcgatcccg tccgctttct taccaatcgt tcttctggaa aaatgggcta tgcactcgcc
     3061 gacgccgcag cccggcgcgg cgcgcacgta atcttgatca gcgggcccac gggacttcct
     3121 atacccgaaa gcactgattg ggtgccggtg cgctcaacgg atgaaatgcg aaacgccgtg
     3181 aacgaacgat ccagggaagc agaaatctta atcatggctg cggctgtctc ggactatcgt
     3241 cccattgcaa cgcgaacgca aaagctcaag cgcggcgata gccgtctcat acttgaactt
     3301 gaacccacac ctgacattct cgcggagttg ggcgctgagc ctctccgacg cgaaagcaaa
     3361 cgtatcctcg tcggcttcgc ggcggaaacg tcaagcatcg ccgaaaacgc gcgggccaag
     3421 ttagcgcgca agggtgcgga tatgatcgtc gcaaacgacg tcagtcaaga aggtgctggt
     3481 tttgacaccg acacgaatat cgtcacactt tttcttcgcg atggcagaga agtctctctc
     3541 ccgaagctat cgaaattcga tgtagccaac caggtcctcg accacgtcct ccagcttcaa
     3601 ggtaagtgac gatccggcaa ggatcgtcgc tctacatacc ggatgcagaa ataaagcgga
     3661 gcgagcacga aggcgactga ggtttcagcg ctgtaatctc ggaaccaagg tcttttcgct
     3721 gtacatgccc gcgcgattat gcgtaagccc gacccctgaa gcagctactg ccgtcgtttc
     3781 ggctgatgtg ccgaggacgc tgcgcgcagt atcgagcacc gccaaaacgg ccggagactt
     3841 ttcattcttg cgccacgcca tatacacatc gattttagca tcggggtcgt caagcggaac
     3901 agccaccgcc acattcccgt tatctaggcg agtagaggct tcgtccgcaa ttatgaaaat
     3961 tcccttgttc gccgccagta gaaccttgtg aacctcgcca tgagacaacg gatttgcggc
     4021 catggcgctg acgcgcggtg atatgccagc tttggcgaac agctcgagcg tccgttcgcg
     4081 cagccctcct ccgacacttg cgtctggaag aaaaaaggtc tcggccgcga gttctttgat
     4141 tcgcagcgtc ttgcgttttg ccagcagatt cgctcggctc atcattgcga taaggcgttc
     4201 ctcgtagagg aactcggagt tcagtcgcac tgtattcacg ggcggtcgca ggaaaccaat
     4261 atcgatcttt ccctccgcca gcgccgcgtt ctgcagtgtt gagaaaatgc tatcgca
//
LOCUS       DATNDT010000105        10020 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3608192, whole genome shotgun sequence.
ACCESSION   DATNDT010000105 DATNDT010000000
VERSION     DATNDT010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10020
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3608192"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2142
                     /gene="uvrA"
                     /locus_tag="VN788_03280"
     CDS             <1..2142
                     /gene="uvrA"
                     /locus_tag="VN788_03280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HXU19588.1"
                     /translation="SEGEKAPERLVFNERFECKCGASYQEPEPRLFSFNNPYGACPRC
                     QGFGNTIDFDLDRVVPDKGKSLAEGAIEPWTKPRYKQLAMEMRRYARAKGIPFDVPFR
                     DLSASQRAAIIEGDRKEDFPGVKGFFSWLERKKYKLHVRVFLSRYRGYALCPECHGTR
                     LRAEARAVKVAGRSITEVCQMTVKEARPFFAELHLSPAETAIAEKILDEIQQRLRFLD
                     EVGLDYLTLDRLTSTLSGGEAQRIQLATSLGSHLVGALYVLDEPSIGLHPRDTHRLIE
                     ILKGLRDLGNTLLVVEHDPDTILAADNVLDLGPGAGEHGGEMLFAGTRDELLSDPRSL
                     TARYLRGELRIAPPARRRKAQGRFLKIIGARSHNLKDVDVMIPLGMLVTVTGVSGSGK
                     STLVYDVIYKALEAKRLGGSWRQFCSRIEGAAAIVAVEMIDQSPIGRTPRSNPATYMK
                     AFDSIREVFAATSDAKKRGLGAGHFSFNVPGGRCETCQGDGTVTVEMRFLADVELTCE
                     ECRGTRYKSSVLEVFYKGKNIHDVLQMTVHEALAFFAPHPRVISRLKVLEEVGLGYLR
                     LGQSGTTLSGGEAQRLKLAVHLTRQDNAGVLYIFDEPTTGLHFDDIQKLLTAFRKLIE
                     GGASVLIIEHNLDVIKSADWIVDLGPEGGDAGGRIVAVGTPEQVARNSQSHTGKFLER
                     VLSSRSDGNHNHHQATAPTAHGEGKKAASGF"
     gene            2170..3513
                     /locus_tag="VN788_03285"
     CDS             2170..3513
                     /locus_tag="VN788_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU19589.1"
                     /translation="MSRFSRVLLGCALCIVAGSLPESVAQKRSTTAKHAARDTSAQEL
                     NQLLDAAQQAIDRQDYASAAQNYQDYLTKNPDDATVHYDLGYAYTALQKPADAKSEYE
                     KAIALNPKLGPAYQNLGLTLLPTDPAGALAALQKAAELLPDDARTKWLLGRALEDNRK
                     FAPAIDQFEAAKNLDAKDPDIRISLGEALLRLGRDKEAFAAFQDALTLQPKGHALAAA
                     HLGLGRAAIAQKQLDEGVSELAAYLQMEPGDASVRIDRASALVDLGKDDEALAELDRA
                     AAPGQEQLRALTLRSQIYWEKKRYDDALPVLQKAVALAPRDPDLLARLGRVYLQKKNY
                     PNAVRYLAAAYNLNPNANDLLGDAIEAEYLNKNYTEALAALDALAKREELPLGSWFMR
                     AACYDNLGETVDALNAYQRFLQLNKDENSDMYFIATARSRVLARELQNKKEKKKR"
     gene            3554..4027
                     /locus_tag="VN788_03290"
     CDS             3554..4027
                     /locus_tag="VN788_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19590.1"
                     /translation="MALALAGSSFGQTRIEELRQRFAQESDPVRKAKKMPQLGEAEFH
                     QIQKDVAGEQFTEALALLRQYRGEVQSCLQGLDATGVNAEKHSSGFRQLQISLRESLR
                     RLDTMIPLISPEAQSGFTGTRQALSEMNKHLIEELFPRPKQPPTKGPSPGHKGHE"
     gene            4024..4557
                     /locus_tag="VN788_03295"
     CDS             4024..4557
                     /locus_tag="VN788_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19591.1"
                     /translation="MRCRAIISVALATVGLSALILTRAAIAQRQRPDYLSDGEADQIR
                     NADTPSQRIKLYLSFAEDRLKKFDYELNRKVPEARRGEILNSLLNGYSGCVDDGADQI
                     DLAKEKQVDIHAALKLMQTKYKSFLDQLEMYDKGGPEIDTYRDTLEDAIDGTKDALSD
                     VQDAEKKMLPAPVRRKP"
     gene            complement(4731..4937)
                     /locus_tag="VN788_03300"
     CDS             complement(4731..4937)
                     /locus_tag="VN788_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19592.1"
                     /translation="MVLSGGPVFYPPCSEPTQLQETLARRAGIGKMAVAFEALGWWTP
                     PIKPAQKNRGYSLDDSAWGASQGI"
     gene            complement(5085..5231)
                     /locus_tag="VN788_03305"
     CDS             complement(5085..5231)
                     /locus_tag="VN788_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19593.1"
                     /translation="MGETLLLFAETCRMGLSAAGRKSDRVLDMEHFVVKNVGDNVVRH
                     PWVV"
     gene            5451..7256
                     /locus_tag="VN788_03310"
     CDS             5451..7256
                     /locus_tag="VN788_03310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU19594.1"
                     /translation="MKLQSSRSAESLLRKIERREWVLWSFAILVTLLLSAGLASFIVP
                     MLHERTEEFDSLHLNLAVRGLVGLVLLFDIYTIYQQLQIFRIRHELVRREELFRLISE
                     NAADMIAVVDGDGKRLYNSPAYERILGYTTEELQSTSSLDQIHPDDRNVVEEAAREAR
                     TGGVGRTIQYRMRHKNGAWHILESGASGVLNAHGQVEKLIIVNRDVTARRNLEDQFRQ
                     AQKMEAVGRLSGGMAHDFNNLLGVIIGYAEFLQEQLDPENTLRSSVDEILNAGKRAAA
                     LTRQLLAFSRQQVLDPKVIDLNGAVSDMEKLLRRLIGEDVELSTQLVSNLGRVKADQG
                     QLEQVVMNLAVNARDAMPQGGKLIIATENMVMDEAFVRRYPYPVQPGPYVCLSVTDSG
                     IGMDAETKARAFEPFFTTKEKGKGTGLGLSTVYGVVKQSGGYIDIISSPGAGTTFKIY
                     LPRIDEAVNLPAAGQGLAGSFEGKETILLAEDEASLRRLTRTTLEQSGYKVLEAKDGV
                     EALTVSEACDLPIDLLLTDVVMPGMGGRALAQELIRRRPEIRVVYMSGYTGQGVGTQG
                     PIDPGSDFLPKPFTRVVLARKIREALDRRMVAQSK"
     gene            7253..7981
                     /locus_tag="VN788_03315"
     CDS             7253..7981
                     /locus_tag="VN788_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19595.1"
                     /translation="MNVLTSNRSVVAEDGALKALKLLVVDDDESIRGLLRTRLSDSYQ
                     IVETGDPEQALALALEHKPDAILLDLMMPGFSGFELCQSFHTLSYTSRIPIFVITGEA
                     GTKYKEHCESLGARGYFQKPVDFAALKGTLAQELKGKIPERRAHKRVQMRVVLGLRGS
                     DASGKEFSESVTTENISAGGFLCNCSATLVKGTAIDVFLASGGHERYAGRAQVVRKDA
                     PGSPWQRYGFQFIETTADWVLQGK"
     gene            complement(8005..8397)
                     /locus_tag="VN788_03320"
     CDS             complement(8005..8397)
                     /locus_tag="VN788_03320"
                     /inference="COORDINATES: protein motif:HMM:NF024572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19596.1"
                     /translation="MTGRRALLVLMVFLAALPGWAGQQKQKPAAAAQSEQQGNGAGSQ
                     ASAEEDIEVGTFYMHKGDTDAAIPRFQDAIRQQPKLGKPRILLAEAYEKKGDKLRAAK
                     SYKEYLQVFPNAADKKKIEKKIEKLTGR"
     gene            8501..>10020
                     /gene="uvrC"
                     /locus_tag="VN788_03325"
     CDS             8501..>10020
                     /gene="uvrC"
                     /locus_tag="VN788_03325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HXU19597.1"
                     /translation="MDLREKADQLPAQPGVYLFQDAGGAILYVGKARSLRGRVKSYFL
                     ESRWQDAKTGSLVREIADLEYIVVDNEREALALENNLIKRHQPKFNVLLRDDKTYPYI
                     RYTAFEKYPRVYVTRRLVKDGSLYFGPYFPASLAYRLVHLIHKHFLVPSCTVDLTRLH
                     PRPCLQYYIHRCLGPCAGLVLDERYEEAARDVRLFLDGRRNDLSRSLNERMEKAAGEE
                     RYEEAAGYRDLLRTLAEMEERQKIAATQGDDTDVIAWYAEPPQVAANLFHLRGGQVVD
                     RRDIYWENLDEFDPAEFLPSLLKQLYLDAAYMPRFIHVPMDFEDCALLEEVLTDAAGH
                     RVEILNPQRGQKHAFLELVEKNAKHSFVQRFRVLKPSSKAIAEALESALALPETPKRI
                     ECFDISHIQGSDTVASMVVWDDGRMKKSDYRKFIIRGDAMQEGSEKSEGVLRDDFASM
                     REVVERRYRRLRDENKPMPSLILIDGGIGQLHAAAQALEKLEIINQPMASIAKKEE"
BASE COUNT         2267 a         2812 c         2825 g         2116 t
ORIGIN      
        1 tccgagggcg agaaggcacc cgaaagactt gtgttcaatg agcgctttga gtgcaaatgc
       61 ggagcgtctt accaggagcc ggaaccgcga ctattctcgt ttaacaatcc gtatggcgcc
      121 tgcccgcgct gccagggctt cggcaacacg atagatttcg atctcgaccg ggtggtacca
      181 gacaaaggga agtcacttgc cgagggtgcg atcgagcctt ggacaaaacc taggtacaaa
      241 cagctggcga tggagatgcg ccggtacgca cgcgcgaaag ggattccctt tgatgtgccg
      301 ttccgcgacc tttcagccag ccagcgcgcc gcgatcattg agggcgatcg caaggaagat
      361 ttcccgggcg tgaaagggtt tttctcctgg ttggaacgaa agaaatacaa gctccacgtg
      421 cgagttttcc tgagccgata tcgcgggtac gccctttgtc ccgaatgcca cggtacacgt
      481 ttgcgcgccg aagcacgggc tgtgaaggtc gcgggacggt cgatcacgga agtttgccag
      541 atgactgtga aggaggcgcg gccatttttc gcggagctgc atcttagtcc agcggaaacc
      601 gctatcgccg agaaaatact tgatgaaatt cagcaacgcc ttcggtttct ggacgaagta
      661 gggttggatt atcttacgct cgatcggctg acttcaacgc tttcgggcgg tgaagcgcaa
      721 cgcatccagc tggcaacttc tctcggttcg catttggtgg gcgcgcttta cgtgcttgac
      781 gaaccctcga tcggtttgca tccgcgggac acgcaccgac tgatcgaaat tttaaagggc
      841 ttgcgggatt tgggaaatac gctactggtt gtcgaacacg atcccgatac gattctggct
      901 gcggacaatg tattggacct tgggcctgga gcgggcgagc atggcggtga aatgcttttt
      961 gcgggcacgc gcgatgaact tctttcggac ccgcgttcac tgacggccag gtatttgcgc
     1021 ggggagctac ggattgcgcc tcctgcgcgt cgccgcaaag cacaaggacg gtttctaaaa
     1081 ataatcgggg ctcgcagcca taatttgaaa gatgttgatg taatgattcc gctaggtatg
     1141 ttggtgacgg tgacgggtgt ttctgggtcg ggcaaatcca cgctcgtgta tgacgtgata
     1201 tacaaggcgc ttgaggccaa gcgcctcggc ggaagttggc gccagttttg tagccgtatc
     1261 gaaggcgccg ccgcaatcgt tgcggttgag atgatcgatc agtctcccat tggaagaacg
     1321 cctcggtcca atcccgcgac ttatatgaag gccttcgatt cgatacgcga ggtttttgcc
     1381 gcgacttcgg atgccaagaa gcgagggttg ggagctggtc acttttcctt caacgtcccg
     1441 ggtggacggt gcgagacatg ccagggcgac gggaccgtga ccgtcgagat gcgttttctg
     1501 gccgatgtcg agttgacgtg cgaggaatgc cggggcaccc gctacaagag cagcgtccta
     1561 gaagtctttt acaaggggaa aaatattcat gacgtcctcc aaatgacagt gcacgaagca
     1621 ttggcgttct tcgcgccgca tccgagagtg atatctcgtt tgaaagtgct cgaggaagta
     1681 ggtctaggct atttacgcct cggccagtcc ggcaccacgc tctccggcgg agaagcacaa
     1741 cgtctaaaac tcgcggtgca tcttactcga caggacaacg ccggcgttct ttacattttt
     1801 gatgagccga ccacgggtct tcacttcgac gacattcaaa agctactgac tgcatttcgg
     1861 aaattgatcg aagggggagc ctcagtcctt attatcgagc acaacctgga tgtgattaag
     1921 tccgccgact ggatagtcga tctcgggccg gaaggcggcg acgcgggcgg ccgcatcgtt
     1981 gcagtaggca cgccggagca ggtcgcgagg aattcgcaat cacatacggg aaagttcctg
     2041 gagcgggtcc taagctctcg cagcgatgga aatcacaacc atcatcaagc aacggcgccg
     2101 acggcccacg gcgaaggtaa gaaggctgct tcaggattct aaggatctta ctggcgtcca
     2161 gggagaagta tgagtcggtt ttcgcgagtt ctcctcggtt gcgcgctctg catagtcgcc
     2221 ggcagtttac ccgagtcagt ggcgcagaaa cgcagcacga ccgcgaagca tgcagccagg
     2281 gacacctcgg cgcaggagct gaatcagcta cttgatgccg ctcagcaagc gatcgacagg
     2341 caggattatg caagcgcggc tcaaaattat caggattacc tcactaagaa tcccgacgat
     2401 gcaaccgtcc attacgatct gggctatgcg tacacggcac tgcagaagcc cgctgacgcg
     2461 aaatctgaat acgagaaggc catcgctcta aatccgaagt taggtccggc atatcagaac
     2521 cttgggctca ctcttctgcc tactgaccct gcaggcgcgt tggcggcgct ccaaaaggcc
     2581 gcggaactct tacccgatga cgcgcgaacg aaatggctgt tgggcagagc tctggaagac
     2641 aaccggaagt tcgcaccggc gatcgaccaa ttcgaagccg cgaaaaattt ggacgctaag
     2701 gaccctgaca tccgcatatc gctcggagag gcgctcttac ggctcggacg cgacaaagaa
     2761 gcttttgcag cattccagga tgcgctcaca ttacagccga agggccacgc gcttgctgcc
     2821 gcacatcttg gattggggcg agcagcgatc gcccaaaaac aactcgacga aggcgtgtct
     2881 gagctcgcgg cttatttaca aatggagccg ggtgatgcca gcgttcgaat cgaccgtgcc
     2941 tcggctctgg tcgatttggg caaggacgat gaggcgctag cggaactgga ccgcgcggct
     3001 gcacccgggc aggagcaatt gcgagcgttg acactgcggt cccaaatata ctgggagaag
     3061 aaacgatacg atgatgcgct gcccgttttg cagaaggctg tggcccttgc gccgcgcgac
     3121 ccagatcttc tcgcccggct cgggcgggtt tacttgcaga agaaaaacta tcccaatgca
     3181 gttcgctatc tcgccgctgc atacaacctg aatccgaacg caaacgattt gctgggagat
     3241 gccatcgagg ccgaatatct gaacaagaat tacacggaag ctttagcagc gctcgatgcg
     3301 cttgctaagc gcgaggaact tcctctcgga agctggttta tgcgggcggc ttgttacgat
     3361 aatctaggag agacggtcga cgctttgaac gcctatcaga gatttctgca gctaaataag
     3421 gacgagaaca gcgacatgta ttttatcgct actgcccggt ctcgagttct agcgcgggag
     3481 ctacaaaata agaaagagaa aaagaaaagg taactccttg cgtgccacac ttcgcatttg
     3541 cggaattata cttatggcgc tggctttggc cggctccagt tttggtcaga cccgcataga
     3601 ggaattacgg cagcgtttcg ctcaggaatc cgacccggtg cgaaaagcaa aaaaaatgcc
     3661 acaactcggc gaggcggagt tccatcagat tcagaaagat gtcgcgggcg agcagtttac
     3721 cgaggctctg gctctgcttc gccaatatcg tggtgaggta caatcctgcc tgcaaggcct
     3781 ggacgccacc ggcgtcaatg cagaaaagca ctcatcgggt ttccggcaac tacagatttc
     3841 cctccgagaa tctctccgtc gcctcgatac catgatcccc ctcatttcac cggaagccca
     3901 gtccggattc accggcacgc gccaagcgct tagcgaaatg aacaagcatc ttatcgaaga
     3961 gttattccct cgcccgaaac agcctcccac caaagggcct tcgcccggac acaaggggca
     4021 tgaatgaggt gccgcgccat aatctccgtc gctttggcaa ccgttgggct gagcgcactg
     4081 attctcacgc gagcagcgat cgctcagcga caacggccgg attatctcag cgacggtgaa
     4141 gcggaccaga ttcggaatgc cgacactcca tcgcagcgca taaaacttta cctatccttc
     4201 gcggaggaca ggttgaaaaa gttcgattac gaactaaatc gcaaagtgcc ggaagctcgg
     4261 cgcggcgaaa ttctcaatag cctcctaaat ggatactccg gctgcgtgga cgacggtgcg
     4321 gaccagatcg atttggccaa ggagaagcaa gtagacatcc acgccgcctt gaagttgatg
     4381 cagacaaagt acaaatcttt ccttgatcag cttgaaatgt acgacaaagg cgggccagaa
     4441 atcgacacct atcgagacac gctggaagac gcgatcgacg gaacaaaaga tgcattatca
     4501 gatgtgcagg atgccgagaa aaaaatgcta ccggcgccag ttcggcggaa gccgtgaggg
     4561 tgctccgcgg cgcaccctct gccgttcccg gcggagagcg gcttttccag cgaatttaca
     4621 ctcgcctcgg atgccaagga cgtccgccac gttttgtcgt tgaatatcac ccgtattcga
     4681 atctcactca cacaatccgg ctcagggatg atacggccta cgtcaggcta tcagatgcct
     4741 tgggaagcgc cccacgcgct atcgtcgagg ctgtagccgc ggttcttctg agccggcttt
     4801 atcggcggcg tccaccaccc gagtgcctcg aaagctaccg ccatttttcc tatgcccgct
     4861 cgacgcgcca gcgtctcctg cagctgcgta ggctccgagc acggcggata gaacacgggc
     4921 cctccggaaa gcaccacaat ctacagctcc tgtttgatca cttgaatcgc cagtatttca
     4981 agagtgccct ggtagcgcct cgcctagcgt ggagcagccg tgcgtggcgg acgcaattgg
     5041 gctgcttcga tcctgcccta agccagattg ttatgaaccg ttggctagac cacccaaggg
     5101 tgccggacta cgttgtcgcc tacgttcttt accacgaaat gctccatgtc aagcacccgg
     5161 tccgatttgc gtcctgccgc cgagagtccc attcggcacg tttccgcgaa gaggagaagc
     5221 gtttcgccca atacgctctc gctttgagat tcctggcacg cttccccgca agctaagcac
     5281 gctgagcctt tttaccgcta cagttcggtc agtttcggcc gattacctat acagcaagcg
     5341 gccctaactc ttctgcgttt ataggcttag cgggctagcg gatttcaaac gcgacgcaac
     5401 gctgctagga cggagctagc agcgtgggtg gcgtaagcgg cagaaacttt atgaaattgc
     5461 aatcgtcacg cagcgccgaa tcgcttttgc gaaagattga acggcgtgaa tgggttctat
     5521 ggtcgtttgc catcctggtc accctcttac tcagcgccgg tctcgcttcg tttatcgtcc
     5581 cgatgctgca tgagcggacc gaggaattcg atagtctgca cctgaatctg gcggttagag
     5641 gcctggttgg cctggttcta ctctttgaca tctataccat ctaccagcag cttcagatct
     5701 ttcgcattcg ccacgaacta gtccgccgag aggagttgtt ccgtttaatc agcgagaatg
     5761 cagccgacat gattgctgtc gttgatgggg acggaaaacg tctatacaac agcccggctt
     5821 acgagcgaat tctcggatac acaaccgagg agctccaaag cacgtcttcg ctcgatcaga
     5881 ttcaccccga cgatcgtaat gtggtcgaag aagcggccag agaggcgcgc accggcggcg
     5941 tcggccgcac cattcagtac cgtatgcgcc acaaaaatgg cgcctggcac attttggaat
     6001 ccggcgccag cggcgtgctc aacgctcacg ggcaggtgga aaagctcatc atcgtgaatc
     6061 gtgatgtcac cgcgcgcaga aacttggaag accagtttcg ccaagcgcag aagatggagg
     6121 ctgtcggcag gctctcgggt ggcatggccc acgatttcaa caatcttctc ggtgtgatta
     6181 ttggctatgc cgaattcctg caagaacagc tcgatccaga aaatacgctt cgcagcagtg
     6241 ttgacgaaat tttgaacgca gggaaacgtg cggccgccct cactcgccaa ctcctcgcct
     6301 tcagccggca gcaggtcttg gatcccaagg tcatcgacct aaatggcgct gtttccgaca
     6361 tggaaaaact cctgcgccgc ctcatcggcg aggacgtcga gctatctact caattggtgt
     6421 cgaatctggg ccgggtgaag gccgatcaag gccaactcga gcaagtggta atgaacctcg
     6481 ccgtcaacgc gcgcgacgcc atgccgcagg ggggaaagtt gatcattgca acagaaaaca
     6541 tggtaatgga cgaagccttt gtgcgccggt atccctatcc ggtgcaaccc ggcccgtatg
     6601 tttgcctcag cgtcaccgac agcggcatcg gtatggacgc cgaaacaaaa gcgcgcgcct
     6661 ttgagccttt cttcaccaca aaggaaaagg gcaaaggtac ggggctcggc ctgtccaccg
     6721 tctacggcgt ggtcaagcag agcggcggat acattgacat cattagctcg cccggagcgg
     6781 gcaccacgtt caagatctat ttgcctcgaa tcgacgaggc cgtcaacctc ccggctgccg
     6841 gccagggctt agccggttcg ttcgagggaa aagaaaccat cctgctcgcg gaagacgaag
     6901 catcgttgcg aaggttgacg cgcaccacac tcgagcaaag cggctataaa gtactcgaag
     6961 ccaaggatgg cgtcgaggcc ctcaccgtga gcgaagcctg cgatttgcca attgatctgc
     7021 tgctgactga cgtcgtaatg cccgggatgg gtggccgtgc gctcgcacag gaattgatcc
     7081 ggcggcgccc tgagataaga gtggtctaca tgtctggtta taccggacaa ggggtcggca
     7141 cccagggacc aattgacccc ggcagtgact tccttcccaa gccgtttacg cgggtggttt
     7201 tggctcgcaa aatacgagag gcgttggatc ggcgtatggt ggctcaatcg aaatgaatgt
     7261 ccttacaagt aatcgttccg tggtggcgga agatggcgct ttgaaggcgc taaagctgct
     7321 ggtcgtggac gacgatgaat ccattcgtgg attgcttcgc acgcgccttt ccgacagtta
     7381 tcaaatcgtc gaaaccggcg atcccgagca ggctctcgcg ctcgccctcg aacacaaacc
     7441 tgatgcgatc ctgctcgatt tgatgatgcc cggattctcg ggcttcgagc tttgccagag
     7501 ctttcatact ttgagctaca cctcccgcat tccaatcttc gtaatcaccg gcgaggcggg
     7561 aacgaaatac aaagagcatt gcgaaagcct cggtgcaagg gggtattttc agaagccagt
     7621 ggactttgcc gccctcaaag gaacactggc gcaggaattg aagggaaaga tacccgaacg
     7681 ccgcgcgcac aagagggttc agatgcgcgt cgttctgggt ctgcgcggat cggatgcaag
     7741 cggcaaggaa ttcagcgagt ctgtaacaac ggaaaacatc agcgccggtg gttttctttg
     7801 caactgctct gcaaccctgg tcaaaggcac tgccatcgac gttttcctcg cgtccggcgg
     7861 tcacgaacgc tacgcaggac gggcccaagt agtgcgcaag gacgcaccag gctcgccttg
     7921 gcagcgctac ggatttcagt tcatcgagac aacagccgac tgggtgctgc aaggcaaata
     7981 atccccatct tcatgggaca aaagttagcg gcccgtcagc ttctcgattt tcttttcgat
     8041 tttcttcttg tccgccgcgt tgggaaacac ctgaagatat tccttgtagg atttcgctgc
     8101 acggagttta tcgccttttt tctcgtaagc ttctgccagc agaatgcgag gcttgcctaa
     8161 cttcggttgc tgtcgaatgg cgtcttgaaa gcgcggaatc gctgcgtcgg tgtctccctt
     8221 atgcatgtag aacgtaccca cttcaatatc ttcctccgcg gaggcttgac tacccgcgcc
     8281 atttccttgc tgttcggact gtgccgcggc cgcgggcttc tgcttttgct gcccggccca
     8341 tcccggcaaa gcggctagga aaaccatcaa taccagtagt gcgcgtctgc cggtcatcgg
     8401 attttccgtt ccttggaatg cctttgctat catagaagtt gccccaaacg gctcacgcga
     8461 gccgcaccgt ccaaatttcg atcgctgtcg cttctggcaa atggacctcc gcgaaaaggc
     8521 cgaccagctt cccgcacagc cgggcgtgta tctattccag gatgcgggcg gcgcgattct
     8581 ctacgtcggg aaagcgcgct cgctgcgggg acgcgtcaag tcctacttcc tcgaatcccg
     8641 ctggcaagac gccaaaacgg gctcgctcgt ccgcgaaatc gccgatctag aatatatcgt
     8701 ggtggacaac gaacgcgagg cgctcgcgct cgaaaacaat ctcatcaagc ggcaccaacc
     8761 gaagttcaac gtactgctgc gggacgacaa aacatatcct tacatccgct acaccgcatt
     8821 cgagaaatat cctcgcgtgt acgtcacgcg ccgcctcgtc aaagatggct cgctttattt
     8881 cggcccgtac ttccctgcca gcctcgccta ccggcttgtg catctgattc acaaacattt
     8941 tctggtgccg tcttgcacgg tagatttgac tcgcctccat cctcgtccct gccttcagta
     9001 ttacatccat cgctgtctcg gtccctgcgc cggccttgtg ctggatgagc gttatgaaga
     9061 agcggcgcgc gacgtccgct tattcctgga tggccgccgc aacgatctct ctcgtagttt
     9121 gaacgagcgg atggaaaaag ccgcgggcga agagcggtac gaggaggccg cgggctatcg
     9181 cgaccttctc cgtaccctcg ccgagatgga ggagcggcag aaaatcgctg ccactcaagg
     9241 cgatgatacg gatgtcatcg cctggtatgc cgaaccaccc caagtagcgg caaatctttt
     9301 ccatcttcgc ggcggacaag tggttgaccg ccgcgatatc tattgggaaa accttgacga
     9361 atttgatcca gcggagtttt tgccctccct gctgaagcag ctttatctcg atgccgccta
     9421 catgccgcgc ttcattcacg tgcccatgga ctttgaggat tgcgcgctgc tcgaagaagt
     9481 cctcaccgat gccgcggggc accgcgtgga aatcttgaat ccgcagcgcg gccagaagca
     9541 cgcgttcctg gaattagtcg agaaaaacgc gaagcactcc ttcgtccaac gatttcgcgt
     9601 tttgaagccc agctccaagg cgattgccga ggcgctcgaa tctgcgttgg cccttcccga
     9661 aacgccgaaa cgaatcgaat gcttcgatat ttctcacatt cagggctcgg atacggttgc
     9721 gtcaatggtg gtctgggacg acgggcggat gaaaaaatcc gactacagga aattcatcat
     9781 ccgtggcgac gccatgcagg agggatcgga aaaatccgaa ggtgttctgc gcgacgattt
     9841 cgccagcatg cgcgaagtcg tcgagcgaag gtatcgacgc cttcgcgatg agaacaagcc
     9901 catgccaagc ttgattctga tcgacggcgg aatcggccaa ttgcacgctg ccgcgcaggc
     9961 tttggagaaa ctcgaaatca tcaaccagcc catggccagc atcgccaaga aagaggagat
//
LOCUS       DATNDT010000106         4771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1799200, whole genome shotgun sequence.
ACCESSION   DATNDT010000106 DATNDT010000000
VERSION     DATNDT010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4771
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1799200"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..924
                     /locus_tag="VN788_03330"
     CDS             <1..924
                     /locus_tag="VN788_03330"
                     /inference="COORDINATES: protein motif:HMM:NF023899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HXU19598.1"
                     /translation="PAGITGEACYYYRAMAWQLRQSGKNSEWKKVADESLYVPFFIED
                     ATGDALVDPQGAELDIHRNFKDEFGGSFFGSHDMTPENVNAFLLRYGLSGSEKIRLEE
                     YCIKPGDPLFVLGTLCQNEAAARWTPCPHVAALGSSFKSRLNFFGPTSSSMFRALGWT
                     SGLKVPVDANVNTSIAGATESASSAAGNWSSVSLEEEAMHSMSATSNGKTAGTATTSA
                     PQAPSGIASVETETAPPETTFGAQSATGFNMHPSVAIGKGTNHDFFMISWHSQREVVQ
                     SLAWKSALCIWGGPALTLACAYFLVLSLGWM"
     gene            950..1522
                     /locus_tag="VN788_03335"
     CDS             950..1522
                     /locus_tag="VN788_03335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003284216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HXU19599.1"
                     /translation="MSLIPWLILLFVVGGAVAYIVTLYNGLIAVKNNVDKAWANIDVL
                     LKQRHDELPGLIEVCKGYMKYESETLQRVTQARNAYAQAATVDQKVQASADMTAALGR
                     LIATAENYPDLKANANFTHLQGRITELESEIADRREFYNDSVNTFNIRIQEMPDAILA
                     GPMNLHPHQMFQVAEADKAPVAISFSDVSH"
     gene            complement(1543..2268)
                     /locus_tag="VN788_03340"
     CDS             complement(1543..2268)
                     /locus_tag="VN788_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HXU19600.1"
                     /translation="MSPSQRKPASKAHDSEFLLLVRRKLARVADARKAPMMQAYMKSA
                     MPYHGVQTVLMRQTCEKLFANLNLSTRDLWQRTVLDLWRGAKYREERYAAIALSGIRR
                     AAEFQTLAAMPLYKEMIVTGAWWDYVDEIAANRVGPILRGDPAPMKKKMLAWSKCENM
                     WKRRTSILCQLRFKQDTDLDLLYACIEPSLDSKEFFLRKAIGWALRQYAWTDAAEIRR
                     YVRKQKVRLSNLSQREALKNLGK"
     gene            complement(2265..3977)
                     /locus_tag="VN788_03345"
     CDS             complement(2265..3977)
                     /locus_tag="VN788_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXU19601.1"
                     /translation="MANRRAAPEAITAIGIAMLLGIASGHAARGQNGSDVTLAHFSGE
                     RMLADIRVLSSDRFQGRGPGTEGEKLTIAYVEKQFRAAGLEPGNPDGTYLQKVPMIGI
                     RSSRNEDLAFTGAGAPMHPTLEKDFVAFTRYPVKDARLDAEMVFAGYGVQAPEYKWDD
                     FKGTDVKGKVLVVLVNDPQIKKDGRLDPKIFGGKAMTYYGRWTYKYEEAERLGAAGCL
                     IVHETAMAGYPWQVVRNSWSGENFTLPSADPGAHGSFVQGWLTHEEAQALFKAAGYDF
                     ASLKEKALSPDFRPVALKIRAKIDIHNDIRRIESHNVIAKLSGSDPALKNEYVIYSAH
                     WDHFGIGPEVNGEKIYHGALDNASGSAALMELGRAFASLKTRPKRTILFLSDTGEEQG
                     LLGSAYYAEHPLYPLSHTAADINMDGVNVLGKTRDITEVGMGESSLDGVVAAVAREQG
                     RTVRPDPEPEKGSYYRSDHFSFAKVGVPAFDPGSGIDYVGKPAGWGMKMRNQFTEHDY
                     HKPSDVIKPYWNMDGAEQDCALYFGVGYRVANAPQMPVWKPGSEFKAIREKSLAGNQT
                     HARL"
     gene            complement(3985..>4771)
                     /locus_tag="VN788_03350"
     CDS             complement(3985..>4771)
                     /locus_tag="VN788_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002700500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HXU19602.1"
                     /translation="IDPPLHEFVPHDRAKQEELAKKIGISAEAVARRVEQLHEANPML
                     GHRGCRLSITYPEILEMQVRAIVEAAIDCQKRKIKVLPEIMHPLTMEKKELQILVDAT
                     RRVAEEIISKSKVKLEYLVGTMIELPRAALLADQIAEVAEFFSFGTNDLTQTTMGLSR
                     DDAGRFLPIYVDQAKAAIFKDDPFQTLDVDGVGMLIKWAIERGRKTRPKLKIGICGEH
                     GGDAQSVKFCHRAGMNYVSASPFRVPIARLAAAQAVIEDKKRR"
BASE COUNT          977 a         1437 c         1302 g         1055 t
ORIGIN      
        1 ccggcgggaa tcacgggcga ggcatgctac tactatcgcg ccatggcttg gcaattgcgg
       61 cagtccggaa aaaatagcga gtggaaaaag gtggcggacg aaagcctcta cgttccattt
      121 ttcatcgagg acgccacggg cgacgccctc gtggacccgc agggcgccga acttgatatt
      181 caccgcaatt ttaaagacga attcggcggc tcctttttcg gcagccatga catgacgccc
      241 gaaaatgtga acgcgttcct gctgcgctac ggcctaagcg gctcggaaaa aattcggctg
      301 gaggaatatt gcatcaagcc cggcgatcca cttttcgttc ttgggacgct ttgccaaaac
      361 gaggcggccg cgcgctggac accctgtccg cacgtcgccg ctctcggctc gtcattcaaa
      421 tctcgcctga acttcttcgg gcccacgagc agcagcatgt tccgagcgct cggttggacc
      481 tcgggattga aagtgcccgt ggacgcaaac gtgaacactt ccattgccgg cgcaacggaa
      541 tctgcctcgt cggctgccgg caattggtcg tcggtctcgc tcgaagaaga agccatgcac
      601 tcgatgtccg caacctcgaa cggcaaaacg gccggcaccg ccacgaccag cgcgccgcag
      661 gcgccctccg gaatcgcaag cgtcgaaacc gaaaccgcgc cgccggaaac tacattcggt
      721 gcgcaatcgg cgacgggatt caacatgcat ccttcggtcg ccatcggcaa aggaacgaat
      781 cacgactttt tcatgatttc ctggcacagc cagcgcgaag tggtgcagtc gctcgcctgg
      841 aaatccgcgc tgtgcatctg gggtggccct gcattgacgc tggcctgcgc ttattttctg
      901 gttctgagcc tgggatggat gtaacttcgc gaatcaccaa ggagatctca tgagcttgat
      961 tccgtggttg atccttttgt ttgtagtcgg cggcgcggtg gcctatatcg tcacgctata
     1021 caacggcctc atcgccgtaa aaaataatgt cgacaaggcc tgggccaaca tcgacgtcct
     1081 gctcaaacag cggcacgacg agcttcccgg cctgatcgaa gtctgcaagg gctacatgaa
     1141 atacgagagc gaaacgctcc agcgcgtcac gcaggcccgc aacgcctacg cccaggccgc
     1201 cacggtcgat caaaaagtcc aagccagcgc ggacatgacg gctgcgctcg gccgcctgat
     1261 cgctacggcg gaaaattatc ccgatctcaa agccaacgcc aacttcacgc atctccaggg
     1321 ccgcatcacg gaactggaaa gcgagattgc cgaccgccgc gagttctaca acgattcggt
     1381 gaacaccttc aatattcgca ttcaggaaat gcctgatgcc atcctggccg gccccatgaa
     1441 tctgcatccg caccagatgt ttcaagtcgc cgaagcggat aaagcgcccg tcgccatttc
     1501 tttcagcgac gtctcacact aaaaatgcgg ccaaaatagg gcctatttac cgagattttt
     1561 cagcgcctcg cgctggctga ggttgctcag ccggactttc tgcttgcgca cataacgccg
     1621 gatttcggcc gcgtcggtcc acgcatattg gcgcagcgcc catccgatgg ccttgcgcag
     1681 gaaaaattcc ttcgaatcga gcgaaggctc gatgcacgcg tacagcagat cgagatcggt
     1741 atcctgcttg aaccttagct ggcacaagat ggacgtccgg cgcttccaca tattttcgca
     1801 cttgctccac gccagcattt tctttttcat cggcgccgga tcgccgcgca gaattggtcc
     1861 cacgcgattt gccgcgattt cgtccacgta atcccaccat gcgccggtca caatcatttc
     1921 cttgtagagc ggcatcgccg cgagcgtctg aaattccgcc gcgcgtctaa tcccactaag
     1981 tgcgatcgcc gcgtagcgct cctcacgata tttggctccg cgccagagat cgagcacagt
     2041 cctttgccac aaatcacgtg tggacaaatt gagattcgca aagagctttt cacaggtttg
     2101 gcgcatgagt acggtctgca cgccgtggta cggcatcgcc gacttcatgt aagcctgcat
     2161 catcggcgct tttcgcgcgt cagcgacgcg cgcgagtttc cggcgcacaa gcagcagaaa
     2221 ctcagagtcg tgggctttgc tcgcgggttt gcgctgagat gggctcatag ccgcgcgtga
     2281 gtttgatttc cggcgaggga tttctcgcga atcgctttga attccgaacc gggcttccac
     2341 acgggcattt gcggtgcgtt ggccacgcga taccccacgc cgaaatacag cgcgcaatcc
     2401 tgctcggccc cgtccatatt ccaatagggc ttaatcacgt cactcggttt gtgataatcg
     2461 tgctcggtaa attgattccg catcttcatg ccccatccgg cgggcttgcc aacgtagtcg
     2521 atgccgctgc cgggatcgaa cgcgggcacg ccaactttcg caaagctgaa gtgatctgaa
     2581 cggtaatagg aacccttctc aggttccgga tcgggtcgaa cggttcgacc ctgctcgcgg
     2641 gcaacggcag cgacaactcc atccagggat gattcgccca tgccaacttc ggtgatatcg
     2701 cgcgttttgc cgaggacatt tacgccgtcc atgttgatgt cggcggcagt gtgggagagc
     2761 ggatagagcg gatgttccgc gtagtatgcc gagccgagca aaccttgctc ttcccctgtg
     2821 tccgaaagaa ataggatggt gcgcttaggc cgggtcttca ggctcgcgaa tgcgcggccc
     2881 agttccatca aagcggctga gcccgacgcg ttgtccaggg cgccgtgata aattttttcg
     2941 ccgtttactt ccggaccgat accgaaatga tcccaatgcg cgctgtaaat tacgtattca
     3001 ttcttaagcg cgggatcgct gccagacaat ttcgcaatca cgttgtgcga ttcgattctg
     3061 cgaatatcgt tgtggatgtc gattttggcc cgtattttca gcgctacggg acgaaaatca
     3121 ggactcagcg ccttttcctt gagcgacgca aaatcgtagc ccgcagcctt gaaaagcgcc
     3181 tgagcttctt catgcgtcag ccaaccttgc acgaatgaac catgcgctcc ggggtcggcc
     3241 gatggcagcg taaaattttc tccggaccag ctattgcgca ccacttgcca cggatagccc
     3301 gccatcgccg tttcgtggac gatcaaacag ccggcagcgc ccaggcgctc cgcttcctca
     3361 tatttgtatg tccagcggcc ataatacgtc atggccttgc cgccaaatat cttcggatcg
     3421 agtcggccgt ctttcttgat ttgcggatcg tttacaagga caaccagaac tttccctttc
     3481 acgtctgtgc ccttgaaatc gtcccacttg tactcaggcg cttgcacgcc atatcccgcg
     3541 aaaaccattt ccgcgtccag ccttgcatcc tttaccggat agcgagtgaa cgcgacaaag
     3601 tctttctcga gtgtcgggtg catgggagca cccgcgcctg taaacgccag atcctcattt
     3661 cgactggaac ggatgccgat catcggcacc ttttggagat aagttccgtc cgggttccca
     3721 ggctcaaggc cggcggcgcg aaactgcttc tcgacgtaag cgatggtgag cttttcgcct
     3781 tcggttccgg ggccgcgccc ttggaatcga tctgaagata ggacgcgaat atccgcaagc
     3841 atccgttcgc cggaaaaatg cgcgagcgta acatcggacc cgttttggcc gcgtgcggca
     3901 tgcccgcttg caatccccaa aagcatcgct ataccgattg cggtgatggc ttccggggct
     3961 gcgcgcctgt tcgccattac ggccctacct tcgtttcttg tcttcgatca cggcttgcgc
     4021 cgccgcgagc cgcgcaatcg gcacgcggaa gggcgaggcg ctcacgtagt tcattcccgc
     4081 gcggtggcaa aactttacgc tttgagcgtc gccgccgtgc tcgccgcaaa ttccgatctt
     4141 cagcttcgga cgcgtcttgc gcccgcgctc gatcgcccac ttgatcaaca tgcccacgcc
     4201 gtccacgtcg agtgtctgga aaggatcgtc cttgaagatc gcggccttgg cctggtcaac
     4261 ataaatgggc agaaatcggc ctgcgtcgtc gcgggaaagg cccatggtgg tttgggtgag
     4321 atcgtttgtg ccaaacgaga agaactcggc cacttcagcg atctggtcgg cgagaagcgc
     4381 ggcacgggga agctcgatca tcgtgcctac aaggtattca agttttactt tggattttga
     4441 aatgatctct tcggctacgc gccgcgtggc gtcgaccaga atttgcagct cttttttctc
     4501 catggtcagc ggatgcatga tctcaggcaa tactttaatc tttctctttt ggcaatcgat
     4561 ggctgcttcg acgatcgcgc gaacctgcat ctccagaatt tccggatagg tgatcgaaag
     4621 cctgcaaccg cgatgcccca gcatcgggtt cgcttcgtga agttgttcca cccggcgtgc
     4681 cacagcctcc gccgagatgc cgattttctt cgctagctct tcctgcttgg cacggtcgtg
     4741 cgggacaaac tcgtgcaagg gcggatcgat c
//
LOCUS       DATNDT010000107         5464 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_617146, whole genome shotgun sequence.
ACCESSION   DATNDT010000107 DATNDT010000000
VERSION     DATNDT010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_617146"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..749)
                     /locus_tag="VN788_03355"
     CDS             complement(312..749)
                     /locus_tag="VN788_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU19603.1"
                     /translation="MAISVGKSQDATVSDPNIVPLIDVLLVLIIIFLVITPKVPTGLP
                     TLIPQPVPPQWKLEPAEPHTIVVQVMKGGKLMINQEQSDWNVLGTRLSDIFKERADKV
                     AFVKGAEEVPFAEVARAIDIMRGAGIVHVGLITDKNKLHNGSS"
     gene            983..2185
                     /locus_tag="VN788_03360"
     CDS             983..2185
                     /locus_tag="VN788_03360"
                     /inference="COORDINATES: protein motif:HMM:NF042637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6569 family protein"
                     /protein_id="HXU19604.1"
                     /translation="MRTDRMRTFSLLSLPIFILLAALWALGNTDSRANATKGSQSFSQ
                     MWRLTQPVTYENMTIFPIVAAQNPDTTAFVTLDDALASGDVVITEQGSYLRRTRDGVR
                     QVPYGSGPEVNQLVLVNRGKRPLILLAGEVVSGGKQDRIIGKDRIVPAGAVPLPLDVF
                     CVEHGRWTGDSDKFSGAQLMVHPTVRKEAAIEQDQSKVWAAVRGQTTQAGNGLAARRG
                     VAGSVLGGTLGSGQSSTAPPPISPLRLDSTIASEAPTQAYAKIYKSSPIGTTVESFAE
                     EMQRRFQRVTEKMKGEHVVGVVIAYGGEVAWSDIFASSQLFDDYWPKLLRSYAVEALT
                     RPEIRRVASLDDARDFLRPPTGHVHEESDPGVYRWTEQNEGRITSIVLEALEPKPITL
                     HWLKVAKE"
     gene            2507..3136
                     /locus_tag="VN788_03365"
     CDS             2507..3136
                     /locus_tag="VN788_03365"
                     /inference="COORDINATES: protein motif:HMM:NF025687.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HXU19605.1"
                     /translation="MRNPTPGASPRLKARIAGAIYLVIFIVAPAGAATATAARMVITL
                     TCDTGVALIFYSLFKPVSRNLSMFAAIFRLILVAIMAVSSLNYFGALNLFNAGHSSAT
                     FNRGEEISLVPFGIHCLLIGYLIFRSSFLPRILGVLMMFAGLGWVTFLSPAFANHLYP
                     YILAPGILGEGALTLWLLVMGLNEQRWREQARAAGLLSPEKANVSGQAG"
     gene            3501..4199
                     /gene="mtgA"
                     /locus_tag="VN788_03370"
     CDS             3501..4199
                     /gene="mtgA"
                     /locus_tag="VN788_03370"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HXU19606.1"
                     /translation="MPDTETTAPHRTGFLRAFIRWLVIGVVLLWSVAALILVAARWIN
                     PPTTAVHIERHLQAWMHGKPYRERYKFVPLRQISTDLQHAVIAAEDARFYQHHGFDWH
                     QIQIDAEDDLEGDRTRGASTITQQLVKNLFFGTGRSILRKGAEATLVPVAELVLGKRR
                     ILEIYLNVVEWGPGIYGAEPACRYYDKTAARNIGRQDAARLAAILPAPLKRRPERMNR
                     YSAIILKRMDQMGW"
     gene            4454..>5464
                     /locus_tag="VN788_03375"
     CDS             4454..>5464
                     /locus_tag="VN788_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19607.1"
                     /translation="MKPLRPKYYAAGLLCLLVALPVSAQKSGPSGGGSKPSQPGAPVS
                     PGQPMGNNQPMTSQPQMQTPTYVRGRVLMDTGQPVPEPVSVGLDCGAQLRQAIYTDQK
                     GYFEFVLGAGPQGNMDMSASNDSTGLRGSSMGGPGGFGGMSGTGDVANSLRGCELQLS
                     VAGYEPLTKEITDPGDIRGVDAGTLQLRRLAGVEGSSISVTSMLVPSKAHKEFDKAEN
                     DLRHNHQASATQHLEKAVSEYDKYAAAWNLLGGIYSSSGEKEKADQAYAKAIASDSHY
                     VPAYVGLASLQLRDAEYESARDTAGKALALDPQTAAASLVQAAADLSLNQLDDAEKIA
                     RNA"
BASE COUNT         1186 a         1542 c         1628 g         1108 t
ORIGIN      
        1 tgggtatttg agctgccgga gaagcatgcg ggcgaggctg ggcactaggt ctaagcgggc
       61 tggacatggt tgcagcacgt ccgggcgagc ccgaacggca aacagcggga gctcgctccc
      121 gcactccaca cggagtgagt aagctaagcg gaggtagcgg agcgatgccg ggctaaagcc
      181 gctggcgcta cacaggcaac ggcaaacgcc ctgaagaaga gagctgaccg aggactccaa
      241 aaagcaattc ttagtagtgc ggaagggccg cttccgttga gccgaatgaa atctaaagtc
      301 caaaatacta attaggagga tccgttgtgg agcttgtttt tgtcggtgat taggcccacg
      361 tgaacgattc ctgctccacg cattatgtca atcgcgcgcg ccacctccgc gaacgggact
      421 tcttcggcac ctttgacgaa agccacctta tcggcgcgct ccttaaaaat atcggagagg
      481 cgagtgccga gcacattcca gtcactctgc tcctggttaa tcattagctt cccacccttc
      541 atcacttgaa ccacgattgt atggggctcg gctggttcga gtttccattg cggcggaaca
      601 ggctgcggaa tgagcgtcgg cagtccagtg ggcaccttcg gcgtgatcac caagaagatg
      661 atgatcagga ccaacagcac gtcaatcaat ggaacaatgt tgggatctga aactgttgca
      721 tcttgactct tgccaactga aattgccatg agcgactcct tataggaagc gctgccgcgt
      781 ccgagccaca cggcgaacga gccgtggcgc aggtttgttc gtctgcggtt tttgacaccg
      841 acgctcgcaa tttgtgccga cggacgcgtg aaatcccgct agaaaatcct gcaagaaatt
      901 ggctctaggc cgttctcacc gaagagaccg cgaaagagct gtcgccggtg tctaactttt
      961 tcgagcgggc aaggaggcga ttatgcgaac ggaccgtatg cgtacgtttt cgttgttgag
     1021 ccttccgatc ttcattctac ttgcagcgtt gtgggcgctg ggtaacaccg actcgcgtgc
     1081 gaatgcaaca aaaggcagcc aatctttctc gcaaatgtgg cggctgacgc aaccggtcac
     1141 ctatgaaaac atgacgatat tccctatcgt cgcggcgcaa aaccccgaca ctaccgcttt
     1201 cgtaactctt gatgacgctc ttgcttctgg cgacgttgta atcacagagc agggcagtta
     1261 cctccgcaga acgcgcgacg gcgtgcggca agttccctac ggctcgggtc ccgaagtaaa
     1321 tcagttggtg ctggtgaacc gcggcaaacg gccactcata cttttggcgg gagaggtcgt
     1381 ttcaggcggg aaacaggacc gcattatcgg gaaggatcgg atcgtgccgg caggagccgt
     1441 gccgctaccg ctcgacgtct tttgtgtgga gcatgggcgt tggacgggtg attccgataa
     1501 gttttcaggg gcgcagctca tggtccatcc gacggtccgc aaagaggcgg ccatcgagca
     1561 agatcagagc aaggtctggg cagcagtccg cggccaaacg acacaagctg gcaacggctt
     1621 ggccgcaaga agaggagtgg ctggaagcgt tctcggaggc accctcggat ccggtcaatc
     1681 aagtacggcg ccgccgccaa tttctcccct tcggctcgac tcgacgattg cttccgaggc
     1741 tccgacgcaa gcctacgcga agatctataa atcgtcgccg atcggaacaa ccgttgaatc
     1801 attcgcagaa gaaatgcagc ggcgcttcca acgggtaacc gaaaaaatga agggcgaaca
     1861 cgtcgtgggg gtagtaattg cttacggagg cgaggttgcc tggagtgaca tcttcgcgtc
     1921 ctcgcaactt ttcgatgact actggccgaa gctgctccgc agctacgccg tcgaagccct
     1981 gacgcgtccc gaaatcaggc gagtcgcttc gctggacgat gctcgtgatt ttctccgccc
     2041 gcccacaggt cacgtccatg aggaatccga tcccggtgta tatcgctgga ccgaacagaa
     2101 cgaagggcgt attacgtcca tcgtactgga ggccctggag ccaaagccaa tcacactgca
     2161 ctggctaaaa gtagccaagg aatgatcgcg cctgacgtag cagtcgtgac tgccgaaaca
     2221 gcattgagtt gacacattgg acgggtaagc cacaagattc cgtcccagcc taacgattcc
     2281 gcgcggcttt tctggcgtcg tcggcagcgg cggtccgatc gtgggaactt atctcgtggc
     2341 cgtgccggaa attcagaatc caccggacca tggcgcggcg atttgggttt tcgagctgtc
     2401 ggagaaggac gcgggtgcgc agtgacgtgg ggcggtcagt cagttcttgg gatgagtgac
     2461 attcggcgtc gccgggatca ggtagtggca ccactggagg ctgaaaatga ggaacccgac
     2521 cccgggagca tcgccacgcc tcaaggccag gatcgctggt gctatttacc tggtgatctt
     2581 catcgtagct ccagccggtg cagcgacggc gacagcggca aggatggtga taacgctcac
     2641 gtgcgacacc ggtgtagcgc tcatcttcta cagcttgttc aaaccggtga gcaggaatct
     2701 ctctatgttc gctgcaattt tccggctcat ccttgtcgcg attatggccg tcagctcgct
     2761 gaactatttt ggcgccctga acctcttcaa cgcgggacac tcgtcggcca cgtttaacag
     2821 aggcgaggag atctcccttg tgcccttcgg catccattgc ctcctaatcg gttacctcat
     2881 tttccggtcg tctttcctgc ctcgcatcct cggcgtgctg atgatgtttg ccggtttggg
     2941 ttgggtgacc ttcctgtcac ctgcgtttgc aaatcatctg tacccctaca ttctggcccc
     3001 cggaatcctc ggcgaaggag cgctgaccct gtggctcctc gtgatgggct tgaacgaaca
     3061 acgatggagg gagcaggcgc gcgcagcggg acttttgtcg cctgaaaaag ccaacgtttc
     3121 agggcaggca gggtaaacaa gctcccgcac tccacacgat tggggtgaat gggctgagcg
     3181 gaggcagcgg agagatgccg ggctgaagcc gctggcgcta cataggcaac ggctccgcgc
     3241 taaagtgcgc cacatcaaag ctggcatttg ccggagcgct gaagcgctcc tcccccgcat
     3301 gaatgcgggg gcttcgacca aagcggccag gctccgactg aaaatgccca gggctaaagc
     3361 cctttttcat tttagggctg tttcccccgc taaatgcggg ggcttccacc gaatacttcc
     3421 accgaatact ccactaaata cgaccgacga atccaccagg agtgaccccg cccgggctat
     3481 tattctgaaa tctgactgca atgccggaca cagagaccac ggccccgcat cgcacgggtt
     3541 ttctccgagc ttttattcga tggcttgtca ttggcgtggt gcttctttgg tcggttgctg
     3601 cgctgatcct tgtggccgcc cggtggatca atccgccgac aaccgcggta cacatcgagc
     3661 gccatctaca ggcctggatg cacggcaagc cgtatcgcga acggtacaaa ttcgttccgc
     3721 tcaggcaaat ctcgaccgac ctccagcacg ctgtgatcgc cgcagaggac gcgcgcttct
     3781 atcagcacca tggattcgat tggcaccaaa tccaaatcga tgccgaagac gatttggaag
     3841 gcgatcgcac tcgcggggct tccaccatca cgcagcaact tgtaaaaaac ctattcttcg
     3901 gaacgggccg gtcgatcctc cgcaagggcg cggaggccac gctggtcccg gtggccgaat
     3961 tggtgctcgg caaacggcgc attctggaga tatacctcaa cgtggtcgaa tgggggcctg
     4021 gcatttatgg ggcagagccg gcgtgccgct actacgacaa aaccgccgcg cgaaatatcg
     4081 gccggcagga cgcggcgcgg ctcgctgcaa ttctgccggc tccgctgaaa cggcgacccg
     4141 agcgcatgaa tagatacagc gcgattattc ttaagcggat ggaccaaatg ggttggtaat
     4201 ggtggttaac atttcaacgc gcggctctta gggcaaccgt gattgacgtg aatgggatac
     4261 gcccgaggga cgccaaaaga tgccgggctg aagccgctgg cgctacatag gtaccgacgc
     4321 ccgaagagtt cgcaaaaagt ggcaggcgac gtcggtctct ccctgctcgc gtgaattctg
     4381 atcttcaggc caaatacggg cgtatacttt gcacgcgaat gtaaggtctg ttcctgccgg
     4441 tgtgaggtgt tccatgaaac cattgcgccc taaatattac gccgctggat tgctctgttt
     4501 gctcgtcgct ttgccggtgt cggctcagaa gagcggaccg tcgggaggag ggtctaagcc
     4561 atcccagccc ggagccccgg tgtcgcccgg gcagccgatg gggaataacc agccgatgac
     4621 atcgcagcca caaatgcaga ctccaactta tgtccgggga cgagtcctta tggataccgg
     4681 ccagccggtc cccgaaccag tttcggtggg actcgactgc ggggcgcagc ttcggcaggc
     4741 gatctacacc gaccagaagg gatacttcga attcgttctg ggcgccggcc ctcaaggcaa
     4801 catggacatg agcgccagca acgattcaac ggggctacgt ggtagcagca tgggcggtcc
     4861 cggtgggttt ggcgggatga gcgggaccgg cgatgtggcc aatagtttga ggggttgcga
     4921 gctgcaattg tcggtcgcgg gctacgagcc gctgaccaag gaaattaccg accccggcga
     4981 catcaggggc gtcgatgcgg gaactctgca actgcgtcga ttggccggag tcgaaggttc
     5041 gtcgatcagc gttacctcta tgctggttcc gagcaaggct cataaggaat tcgacaaggc
     5101 cgagaacgac ctccgccaca atcaccaggc ttcggcaacg cagcatctgg aaaaagcagt
     5161 ctcggaatac gacaaatacg ccgctgcatg gaacttgttg ggtgggattt actcgtcttc
     5221 cggcgagaag gaaaaggccg accaagccta tgctaaggcg attgccagcg actcgcacta
     5281 cgttcctgcg tatgtcgggc tcgcatcgtt gcaattgcga gatgcggaat atgagagcgc
     5341 ccgagacact gccggtaagg ctctagctct ggatcctcaa accgcagccg ctagcttggt
     5401 ccaagcggcg gcagatcttt ctctgaacca gctggacgac gccgagaaga tcgcgcggaa
     5461 cgcc
//
LOCUS       DATNDT010000108        12521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2316328, whole genome shotgun sequence.
ACCESSION   DATNDT010000108 DATNDT010000000
VERSION     DATNDT010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12521
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316328"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1940
                     /gene="argS"
                     /locus_tag="VN788_03380"
     CDS             <1..1940
                     /gene="argS"
                     /locus_tag="VN788_03380"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HXU19608.1"
                     /translation="LAMGEVASPVCFELAKRLKKPPRALAQEIANSLKPIEGVARVEV
                     AGGGYLNLFLDRAAFFRAAGEETGRQTAGASAPSSQGVQAKTVVEHTSINPNKAAHIG
                     HLRNAVLGDTFSRILRYAGRCVEVQNYIDNTGVQVADVVLGFLRLEPKTLDEVRALAA
                     APKFDYFCWDLYTRVTQWLAEDKSRLEIRSRTLKEIEEGQGDAAKMAEVVSTSIVHCH
                     LRTLDRLGIDYDLLARESDVLHLKFWDAAFELLKQSGAIELAAAGKNAGCWVMQLPSD
                     KETSETGVDAEAAEEPTELAEAKIIVRSNGTVTYVGKDIAYHLWKFGLLNRDFHYRRF
                     HKHPDGQEAWITDSTADDAGAPEFGHAHEVFNVIDSRQAYPQQVVVAGLRALGHAEEA
                     DHLKHFSYNVVALTPRCALEMGYEIPPEDAKKPYIEVSGRKGQGVKADDLIDQLIARA
                     GEETASRHPEMEGTAEFNDMVNKIAVGALRYFLLRFTRSTVIAFDFKDALNFEGETGP
                     YAQYAVVRVNGILRKSAELHPEFESVDAANFTRLKSGELDTAKFLAMPAGDDLWELIV
                     LAGSLDARVDAAIGGQEPAFLARYAFELAQAFNIFYHKHHILSEEDAEKRAFLLRITS
                     LVREQLVITLGLLGMAAPEKM"
     gene            complement(2076..3569)
                     /locus_tag="VN788_03385"
     CDS             complement(2076..3569)
                     /locus_tag="VN788_03385"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HXU19609.1"
                     /translation="MQIQLETQPFTSIQADALVTYVFDKENKFDGLLGEMDGAMNGRL
                     SSLAANGEITGKMLETLLLHFPEGLAAQKLLLVGAGKSDKFSASDLRKIAGTAVRQLK
                     SRGAKKIVFLAREGNRDSAAAQAVVEGLIAADFESDKYRSEKKNREVQSIGLAGFDAG
                     LGVDLTAAINQGTVIAESQNFARDLINEPSNKMTPRILAARAEAMAKEAGLGIEILDE
                     RKIAELKMGALIGVAQGSVEPPRVIVLRYEPLNPKLGAPVLGLVGKAVTFDTGGISIK
                     PANNMEKMKYDMGGGATMLGAMRAIAALKPSISVIAVIPSTENMPGGRAQKPGDVQVA
                     MSGKTIEVINTDAEGRLILADGVFYARKLGCTHLIDAATLTGAIEVALSNVHVGAFGT
                     PREYLDEFLQSAKATGEKMWPMPIDDEYQQMIKSDIADIRNTGSGKGGGASTAAWFIK
                     EFVDDTPWVHLDIASTCWVDEGRPWLAKGPTGVAIRSIVDFALKLKT"
     gene            complement(3609..4094)
                     /gene="smpB"
                     /locus_tag="VN788_03390"
     CDS             complement(3609..4094)
                     /gene="smpB"
                     /locus_tag="VN788_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXU19610.1"
                     /translation="MNSEKKGDKERNKIVAQNRAASYNYDLLDRLEAGLVLVGTEVKT
                     LREGKGTLREAYAEIRAGEPWLVNFHIPEYQPGGPRNHDPLRKRKLLLNRREIDKLIV
                     QTQQKGMTVVPLKIYFRDGIAKCELAVARGKKFHDRRESERRKEAKREADDAIYRSRR
                     R"
     gene            4304..6559
                     /locus_tag="VN788_03395"
     CDS             4304..6559
                     /locus_tag="VN788_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HXU19611.1"
                     /translation="MRRSPLLAILALATVVLSGCPKGNQDYNAGRKAETLQDYDTALV
                     DYQRALRTDPSNAEYKLREMHTRFAAGQFHLEQGEKALKTNDLQLAVAEFQKAAAIDP
                     SNSAADQELKKTMDLLAAKNATSTSKAINPNPSEDSELLAAPPQLKPVSTELINLKMT
                     NDSRVVFETVAKLAGLSVIFDPDFTSRRIAADLPNVTLEQALDAVAFESKAFWKPLTS
                     SVIIVAPDNPQKRRDLEDEEVRTFYLSNTLTPQDLTEVVTGLRQLLDMRRVQQVNAQN
                     AIVIRDTPDKLMLASKIIRDIDKAKPEVLVHVQVLTASVDRLRDLGIMPGQSATVTFT
                     PRSALQPNGGTSTSGSSSSGSGTSNPLQITLDNLRNLSGGDYSVTLPGATANAILTDN
                     KTRIIQDPEVRVSDGEKATLKIGDRVPVATGSFQAGVGVGTSSINPLVNTQFQYIDVG
                     VNIDVTPRVHPDGDVSMKLSIEVSQITGTSNIGGINQPVISQRKIEHDIRLKNGEVSV
                     LGGLIERTQTKNINGIPGVAQLPLFRYLFSDNSNDVKEDEVLIVLTPHVLRLPSITAE
                     NLRTLAAGTDTAARVYRDDQEATPTPAIPNAPSTSIPPQTVPPGGATPAAQLHFDPPA
                     VTLKPGDTTTLGLAISNVSDLYSIPLLIHYDPAVVQVEEVRNGGFLSGGTQEIAIVQR
                     VDAQRGEVVVSATRQPNTPGVNGSGTLLGVVVRGVAPGRTALQILQVNAHDSQQRTIP
                     VVSGEATIQVQ"
     gene            6565..7095
                     /locus_tag="VN788_03400"
     CDS             6565..7095
                     /locus_tag="VN788_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXU19612.1"
                     /translation="MSGTSHGTKGIEVKRRARLWGRAQGGEAGLTLLELIVACAILMV
                     LAGVAMPMARVSVVRHKEEMLRYDLREMRDAIDHYKDDADKNLIQVQAGTEGYPPDLQ
                     TLVNGVQLTSAQDKDKKVRYLREIPKDPLTGNQDWGLRSTQDDPDSKSWGGQDVFDVY
                     SQATGTALDGTNYSDW"
     gene            7121..7543
                     /locus_tag="VN788_03405"
     CDS             7121..7543
                     /locus_tag="VN788_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXU19613.1"
                     /translation="MKRAHTTAKRRRHANGGFTLLELMIVAAIIVVLATIGSARYDQA
                     MVRAHEAALSQDLSEMNKAIQNYTADKEAAPTSLDDLVQAQYLGRIPKDPITGNTDWT
                     TENCDLLVNADQTSTGICSVHSSSDKVSPFTNTPYSSW"
     gene            complement(7760..8410)
                     /locus_tag="VN788_03410"
     CDS             complement(7760..8410)
                     /locus_tag="VN788_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXU19614.1"
                     /translation="MPEVFHRRIFLKGPAGRLQASLWTASTAEPGFVAVVCHPHPLYG
                     GTMHNKVVYQAAKALQQRGASVLRFNFRGVEQSEGAHDRGIGEQEDVRAALDYLSAEF
                     PGKAILLAGFSFGSSVGLRVGCEDARATRLIGLGLPADNIDLSYLRSCIKPKLIIQGG
                     QDQFGTRATMETLFATIPQPKYLVFVEGADHFFTGHLNAIGTAIDAWLDRPSPPRD"
     gene            8612..8938
                     /locus_tag="VN788_03415"
     CDS             8612..8938
                     /locus_tag="VN788_03415"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU19615.1"
                     /translation="MANGVERRAARRFSMMLPLKVRFSAENGVTEKSGETRDVSFRGL
                     YFLIEASLETGSSIEFVLTLPQQITLAGDVHIRCYARVLRVDSHDGRRGVAARIERYE
                     FLPASA"
     gene            9047..9733
                     /locus_tag="VN788_03420"
     CDS             9047..9733
                     /locus_tag="VN788_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXU19616.1"
                     /translation="MARHTLLIDADDTLWENNIFFEKAIDDFISQLEHLGYTREYIRH
                     ILNETERRNIRQHGYGVRSFRRSLEETYLKLAGNSARREIVKEIERVALELESTPPHI
                     MDGVPETLVYLSKHHRLILLTKGEPAEQAAKVERSGLQSYFEAIEIVLEKDSGTYGRI
                     IDQFKIVKSHGWMVGNSPRSDINPALQSGLNAVFIPHSATWELEKSEVESGTGKLLIL
                     SAFRDLRGHF"
     gene            9835..10974
                     /locus_tag="VN788_03425"
     CDS             9835..10974
                     /locus_tag="VN788_03425"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXU19617.1"
                     /translation="MIADYRHEFADFEDVTYLNAALQGPMPLVAAHAAEVALEWKKRP
                     DRLPDSAYFDLPDRIREKTAALIGSRADEIAVTTGASAGLACVAAGIDWKPDDEVLVG
                     QGEFPAHFSTWLRYQKAGKLRVRVITPKGRFITADDYIEHIGPQTRVVSASLVRFDNG
                     ARLDARRVAQACEKYGAALLLDVSQCAGAMKLDILDLGAAMAVCSGYKWLLGPYGTGF
                     FWVANEWIERLTLGSVYFMALEGARTFHALPIENQQPAPGVRRWDSAETANFTNLAAL
                     DSSLDFLLRIGTDRIAAHTRSLVDELIERLPGTRCKLASPEECDRRGPYACISASTPS
                     DTSALYEKLRGAQVHVSLRENALRIAPYIYNTREQISQLLEILSE"
     gene            complement(10989..>12521)
                     /locus_tag="VN788_03430"
     CDS             complement(10989..>12521)
                     /locus_tag="VN788_03430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXU19618.1"
                     /translation="APKEAELTKERGFHVDWIRGRVPSKAFAASSLWHVAVIWLLILP
                     IWGGLPEHTPTLAPVQIELTWTGEQPDLPRISLPAAVPSPREKPKNIAPSVPESSGAD
                     ASRARQTILSIPVRVTHPRQALIRPESPSAPPKVVPQLPNIVQWSSDQPQKPKFQLSV
                     ANSAPKIRRRTVRDDAAPEVPNPAKNLVPFNIVSKANAIPRPKMPVSAMSAPATRQRE
                     TRADTAAVPEIGAADTSSSRRIIALSATPSPPAPAAGVPEGNLAARVLTSPDGKKPGA
                     VADAANHSTRPGPGGAAAPSATGGTGAGSNSDSLPAAISITGGGKPSSRGGIAPSAAS
                     RLNTLNLNPPNSYQPPSNSRRGPPDVSGLDPSLPPEKILSGSEVYTMHVNLPNLTSAS
                     GSWILSFSELDEDRPSVAPRERLAGPVPIRKADPKYPPDMIKAHVQGEVVLYAIIRKD
                     GSVDSIQVVRGLDPKLDQNAIQALSEWKFRPATRASHPVDVEAVVHIPFLYQNPRDYN
                     SQ"
BASE COUNT         2677 a         3470 c         3790 g         2584 t
ORIGIN      
        1 ggctggctat gggcgaggtc gcttcgccgg tgtgtttcga actggccaag cgcctgaaga
       61 aaccgccgcg ggccctggcg caagagattg ccaattcgct gaagccgatt gaaggcgtgg
      121 cgcgtgtgga ggtggcgggc ggcggctatc tgaatctatt tttggaccgc gcggcatttt
      181 ttcgcgcggc gggcgaagaa accggacggc aaacggcggg cgcaagcgcg ccatcatcgc
      241 aaggtgtgca ggccaagacg gtcgttgaac acacgagcat caatccgaac aaagcggcgc
      301 acattggaca cttgcgcaac gcggttctgg gcgacacatt ctcgcggatt cttcgctacg
      361 ccggacggtg cgtggaggtg cagaactata tcgacaacac aggcgtgcag gtggcggacg
      421 tggttctggg atttcttcgc ttggaaccga aaacgctcga tgaagtgcgc gcactggccg
      481 ccgctcctaa gtttgattat ttttgctggg acctttacac gcgcgtcaca cagtggctgg
      541 cggaagataa atcgcggctg gagattcgct cgcgaacgct caaggaaatc gaagagggcc
      601 agggcgacgc ggccaagatg gcggaggtgg tgtcgacgtc aatcgtgcat tgccatcttc
      661 ggacactgga tcgattgggg atcgattacg atctgctagc gcgcgaaagt gacgtgctgc
      721 acctgaaatt ctgggatgct gctttcgagc tactgaaaca gagcggcgcg atcgagctgg
      781 cggctgccgg gaaaaacgca ggctgctggg tgatgcagct tccttcggat aaagagactt
      841 cggaaaccgg agtagacgcc gaggccgcag aggaacccac cgaactggcc gaagcgaaaa
      901 ttatcgtgcg ctcgaacggc acggtgacct atgtgggcaa agacatcgcc taccatctgt
      961 ggaaattcgg cttactgaac cgcgattttc actatcgccg ctttcacaaa catcccgacg
     1021 ggcaggaggc atggattacc gattcgactg ccgacgatgc gggcgcgccg gaatttggtc
     1081 acgcgcacga agtattcaat gtgatcgatt cacggcaggc gtatccgcag caggtggtgg
     1141 tggccggatt gcgcgcgctt gggcacgcgg aagaggccga tcacctgaaa catttttcct
     1201 acaacgtggt ggcgctgact ccgcgctgcg cgctggagat gggctacgag attcctcctg
     1261 aggatgcgaa aaaaccttac atcgaagtga gcgggcgcaa aggccaaggc gtgaaggcgg
     1321 acgacttgat cgatcaattg attgcacgag ccggcgaaga aactgcttcg cgacatccgg
     1381 aaatggaggg cacggccgag tttaacgaca tggtgaataa aatcgcggtc ggagcgctgc
     1441 gctattttct gctgcgcttc acgcgttcga ccgtgatcgc attcgatttc aaggatgcgc
     1501 tgaacttcga gggcgagacc gggccctacg cgcaatacgc ggtggtgcgc gtgaacggaa
     1561 ttttgcgcaa gagcgcggag cttcatccgg aatttgaaag cgtggatgcg gcgaatttta
     1621 cgcggctgaa aagcggtgag ctcgacacgg cgaaatttct ggcgatgccc gcgggcgacg
     1681 atttgtggga gttgatcgtg ctggcagggt cgctggatgc gcgcgtggac gcggcaatcg
     1741 gcggccagga gccggcattt ttggcgcgct acgctttcga gttggcgcag gcgttcaaca
     1801 ttttctacca taagcaccac attctttcgg aggaagatgc ggagaagcga gcatttttat
     1861 tgcgcattac gtcgctggta cgcgaacaac tggtgattac gctcgggcta cttggcatgg
     1921 ctgcgccgga gaagatgtga cacttgtcat gcctacatcg aagagagatt cttcgagtgg
     1981 tgttccggcg cttcgcgcaa cgacaaaggc gcgcggggca ctccgctcgg ctcgacgccc
     2041 tttgcgactc gggcaagacg caacaagtgg ggcggttacg tcttcaactt caacgcaaag
     2101 tccacgattg aacgaatggc aacgccagtg ggccctttgg cgagccaggg gcggccttcg
     2161 tcgacccaac aagtcgaggc aatatcgaga tggacccatg gcgtgtcgtc gacaaattcc
     2221 ttgatgaacc atgccgcggt gctggcgccg ccacctttgc cgctgccggt gttgcgaatg
     2281 tccgcaatat cacttttgat catctgctga tattcgtcgt cgatcggcat gggccacatt
     2341 ttttcaccgg tggctttggc gctctggaga aattcgtcga gatactcgcg cggggtgccg
     2401 aacgctccca cgtgaacgtt ggagagggcg acttcgattg cgccggtgag cgtggcggcg
     2461 tcgatcaggt gcgtgcagcc gagcttgcgg gcgtagaaga cgccgtcggc gaggattagg
     2521 cgaccttcgg cgtcggtgtt gatcacttcg atggtcttgc cggacatcgc gacctgaacg
     2581 tcgccgggct tttgggcgcg gccgccgggc atgttttcag ttgaagggat cacggcgatc
     2641 acggaaatag aaggcttcaa cgcggcgatc gctcgcatcg cgcccagcat ggtggcgccg
     2701 ccgcccatgt cgtatttcat tttttccatg ttgttggccg gtttgatgga gatgcccccg
     2761 gtatcgaaag tgacggcctt gccgacgagc ccgagcacag gcgcgcctag ctttggattc
     2821 agaggctcgt agcgcaggac aatcacgcgc ggcggctcga cgctgccctg cgcgacacca
     2881 atcagcgccc ccatttttag ctcggcaatt tttcgctcat ccagaatttc gattccgagg
     2941 ccggcctctt tggccatagc ctcggcgcga gcggccaaga tgcgcggcgt cattttattg
     3001 gaaggctcgt taatcaggtc gcgggcaaaa ttttgcgatt cggcgatcac ggtaccctgg
     3061 ttgatggcgg ccgtgagatc gactccgaga cctgcgtcga atccggcgag accgatcgat
     3121 tgcacctcgc gattcttctt ctcgctgcgg tatttgtcgg attcgaaatc cgcggcgatc
     3181 aagccttcga cgacagcctg cgctgctgct gaatcgcgat tgccttcgcg agcgaggaag
     3241 acgattttct tcgcgccgcg cgacttcaac tggcggacag cggtgccggc gattttacgc
     3301 aggtcagagg ccgagaattt atccgatttg cccgcgccca cgaggagcag cttttgggcg
     3361 gcgaggcctt cgggaaaatg aagcaaaaga gtttccagca ttttgccggt gatctcgcca
     3421 ttcgcggcga gtgagctgag gcggccgttc atcgcaccgt ccatttcacc aagaagcccg
     3481 tcaaacttat tttccttatc gaagacgtag gtaaccaggg cgtcggcttg tatggaggta
     3541 aaaggctgag tctcgagttg gatctgcatg tgtcctttca ctttcgtttt tgtatatgag
     3601 tcccgcggtc agcgccggcg agaacgatag attgcgtcat cggcttcgcg tttggcttcc
     3661 ttgcggcgtt cggattcgcg ccggtcgtga aactttttgc ctctggccac cgctagctcg
     3721 cacttcgcga ttccgtcgcg gaaataaatc ttcagcggaa caacagtcat gcccttttgc
     3781 tgcgtctgca cgatcagctt atcgatctcc cgccgattga gaagcagttt gcgttttcga
     3841 agcggatcgt gattcctggg gccgccgggc tggtactccg gaatatgaaa gtttaccagc
     3901 cagggctccc ctgcgcgaat ctcggcatag gcttcgcgca gcgtgccttt gccttcgcga
     3961 agagtcttga cctcagtgcc taccaggacc aagcccgctt ccagcctatc gagtaggtca
     4021 taattgtagg atgcagcccg attctgcgcg acgatcttat tgcgctcttt gtctcctttt
     4081 ttttccgaat tcatggtgcc gcaatgatag ccgccgcgca caagggcccg caagtaacgt
     4141 cgcctgtgga cgttccgcaa tgaaccagct tgggtgctgt taacgcgcag gtcacaaaac
     4201 aaagatcatt agaaataacc ggcaacgctg acgttttctt gccgcatcat agggcgggtg
     4261 ctagactcgc tggcacccaa gcccaatttt ttggaggttc cggttgcgcc gctcaccgct
     4321 tttagccatt ttggcgctgg ccacggttgt tctaagtggc tgtcccaaag gaaatcaaga
     4381 ttataacgcg ggccggaagg ccgagacatt gcaggattac gacacggccc tcgttgacta
     4441 ccagcgcgct ctgcgaaccg atccctcgaa cgccgagtac aagctgcgcg aaatgcacac
     4501 gcgctttgcc gccggccaat ttcacctgga gcaaggggag aaggcgctga agaccaacga
     4561 tcttcagctt gcagttgcgg aattccagaa ggctgctgcg atcgatccct cgaatagcgc
     4621 cgcggatcaa gaactaaaga agacgatgga tctgctagcc gcaaagaatg cgacatccac
     4681 ttcaaaagcc atcaatccga atccgtcgga ggattcggaa ctactagcgg ctccgcctca
     4741 gttgaaacct gtttcgaccg aattgatcaa tctgaagatg accaacgatt cgagggtggt
     4801 cttcgagacc gtcgccaagc tggcgggatt gagcgtgatt ttcgatccgg acttcacgtc
     4861 gcgccgaatc gcggcggacc tcccaaacgt gacgctcgaa caagcgctcg acgccgtcgc
     4921 gtttgagagc aaggcttttt ggaagccgct cacgagcagc gtgattatcg tggcgccgga
     4981 caaccctcaa aagcgcaggg acctggagga tgaggaggtc cggacctttt acttgtcaaa
     5041 tacgctgacg cctcaggatt tgacggaagt ggtgactggg ttgagacaac tcctcgatat
     5101 gcgccgggta caacaggtaa atgcgcagaa tgcaatcgtg atccgggata ctccggacaa
     5161 actgatgctg gcatcgaaaa ttatccggga catcgataaa gccaagcccg aagtcttagt
     5221 ccacgtgcaa gtgcttactg cgagcgtgga cagactgcgc gatttgggca tcatgccggg
     5281 tcagagcgct acggttacat tcaccccacg cagcgccttg cagccgaacg gcggaacgag
     5341 cacttcgggc agttcaagca gcggctccgg cacaagcaac ccgctacaga tcacgctcga
     5401 taatctgagg aatctctccg gcggggacta cagcgttacg ctaccgggcg cgacggccaa
     5461 tgccattcta accgacaaca aaacgaggat cattcaggat cccgaggtcc gcgtgagcga
     5521 cggggaaaaa gccacgctga aaatcggaga ccgagtcccc gtagcgacag gcagctttca
     5581 ggccggcgtc ggagtgggga ccagctcgat caaccccctc gtgaatacac agttccaata
     5641 catcgacgtc ggagtaaata ttgacgtaac gcctcgcgtg catccggatg gcgatgtcag
     5701 tatgaaactt tcgatcgaag tatctcagat tacgggcacc agcaacatcg gcggcatcaa
     5761 ccaaccggtt atcagccaac ggaagatcga gcacgacatt cggctgaaaa acggcgaggt
     5821 gagcgtgctg ggcgggttaa tcgagcggac ccagacgaaa aacatcaacg gcattccggg
     5881 cgtcgcacag cttcctcttt tccgttacct tttctcggat aacagcaacg acgttaagga
     5941 ggacgaagtg ttgatcgtgc tgacgccgca cgtgctgcgg ctgccctcga tcacggcaga
     6001 gaacctcagg acgctggcgg ctggaacgga cacggctgcc cgcgtatatc gcgacgacca
     6061 agaggccacc cccacgccgg cgattcctaa tgcgccgtcc acgtcgatcc cgccgcaaac
     6121 ggttccgccg ggcggcgcga cccccgcagc gcaactgcac ttcgatccgc cggccgttac
     6181 cctgaaaccg ggcgatacaa ccacgcttgg cctagcgatc tcgaacgtca gcgatcttta
     6241 ttcgatcccg ctcttgattc attacgatcc ggcagtggtc caagttgagg aagtccgcaa
     6301 cggcggtttt ctctccggcg gcacgcagga aatcgcgatc gtgcagcggg tcgatgcgca
     6361 gcgcggcgag gtggtcgtgt ctgcgacgcg gcaaccgaat accccaggcg tgaacggatc
     6421 cggaacgtta ctaggggtcg tggtaagagg cgtggccccc ggcagaaccg ccttgcaaat
     6481 tcttcaggtt aatgcccacg actcgcagca gcgaaccatt ccggtagtct ccggggaagc
     6541 cacgatacag gtacaatagg ctgtatgagc ggtacgtcgc acggcacaaa aggcatcgaa
     6601 gtaaagcgaa gagcgagact gtggggccgc gcccaaggcg gcgaagccgg cctaactctt
     6661 ctcgaactga tcgtggcgtg tgccattctg atggtgctcg ccggtgtagc gatgccgatg
     6721 gcgcgtgttt ccgtcgtccg gcacaaagaa gaaatgcttc gctacgatct gcgtgaaatg
     6781 cgcgacgcga tcgatcacta taaggacgat gcggacaaga acctaatcca agtgcaggcg
     6841 ggcaccgaag gatatcctcc cgacttgcaa acgttggtga acggggttca attgaccagc
     6901 gcacaggaca aggataagaa ggtccgatac cttcgtgaaa tcccaaagga tcccctgacg
     6961 ggaaaccagg attggggcct gcggtctacg caagacgatc ccgattcgaa gtcttggggg
     7021 gggcaggatg tcttcgatgt gtacagccag gcaacgggaa cggcgctcga cgggacgaac
     7081 tattcggatt ggtaataggc gcgctttgcc agaggatttt gtgaagaggg cacacaccac
     7141 ggctaaacga agacgacacg cgaacggagg gttcacgctg cttgaactaa tgatagtggc
     7201 ggcgatcatc gtggtcctag cgacaatcgg gtcagctcgg tacgatcagg cgatggttcg
     7261 cgcgcatgag gcggccttga gccaggacct ttcggagatg aacaaggcga ttcaaaatta
     7321 cactgcggat aaagaggctg cgcctacgtc gctcgacgac ctggtgcaag cacaatatct
     7381 cggaagaatt ccgaaggatc cgattacagg caatacggat tggaccactg agaattgcga
     7441 tttgctcgta aacgccgatc aaacttcaac gggaatttgc agcgtgcatt cttcatccga
     7501 caaagtctcg ccattcacga atacgccgta tagttcctgg taagcgggct cgagaagttt
     7561 ggaccggctc cggcgcacct ccaaaataaa aacgggaggc aaaagcccaa aggcctcgcc
     7621 tcccggggtg ggtggggttt gttaccgaac acccagcatg gaattttggt gttccgcgcc
     7681 tgggtgaatc ggcaaaccgg ccttgagccg gtgccacgta tattccacct ttatcacggg
     7741 gccggaacgg gtgtcaatgt taatcgcgcg gcggggaggg gcggtcaagc caggcatcga
     7801 ttgcggtccc aatggcattc agatggccgg tgaagaaatg gtccgcacct tccacgaaga
     7861 cgaggtactt tggctgcggg atcgttgcga aaagagtctc catagtcgca cgggtaccga
     7921 attggtcctg gccgccctgg atgatcagct tgggcttgat gcaggagcgg aggtagctca
     7981 gatcaatgtt gtcggcaggt aagcccagcc cgatcagcct agttgctcgt gcgtcttcac
     8041 aaccgactcg caggcctacc gacgaaccga agctgaatcc ggcgagaagg atggctttgc
     8101 cgggaaactc cgccgacagg taatccaggg ccgcgcggac atcctcctgc tcgccgattc
     8161 cacggtcatg tgcgccttcg ctttgctcca cgccgcgaaa gttaaaccgc agaacggatg
     8221 caccacgctg ctggagagcc ttggcggcct ggtagacgac cttattatgc atggtgccgc
     8281 cgtagagcgg atggggatgg cagacaacgg ccacgaagcc aggctcggca gtagaagcag
     8341 tccaaagaga tgcttggagg cggcctgcgg gtcctttgag gaaaatcctt cgatggaaaa
     8401 cctcgggcat tcgcgaagcc taaggctccg gcacccatgg tttggccacg gagacactgc
     8461 catgctgccg cgcatgcgga aacatgcatt catcgtagcg gtagtggacg aattggtcaa
     8521 acagcttcgt ccttccttcg ctggggaaat gcgttgcgca gcctagcgag caatggtagc
     8581 tttggcgcag gtgcggggaa gcggagggct catggcgaat ggcgtggaaa ggcgagctgc
     8641 ccgacgtttc tcgatgatgt tacccttgaa ggtacgattc tcagcagaaa acggcgtcac
     8701 cgaaaaatcg ggcgaaacgc gagacgtcag ctttcgcggg ctgtacttcc tgatcgaagc
     8761 gagccttgaa accggcagtt cgatcgagtt cgttctcacg ttgccgcagc agattacgct
     8821 cgcaggggac gtgcacattc ggtgctacgc gcgcgtcctg cgggtggact cgcacgatgg
     8881 caggcggggc gtagcggcgc gaatcgagcg gtacgaattc cttcccgcct ccgcctagga
     8941 tgacgaacca tatcctctac taattggtat ccgcgtaaag agtctcgctg aaaaattcct
     9001 tctcttatct ccttctctga cgcaagatct acgctcgggg tctcgtatgg cgcgacatac
     9061 actgctcatt gacgcggacg acacgctttg ggaaaacaat attttcttcg agaaggcgat
     9121 cgacgatttc atttcccaac tcgagcatct cggttacacg cgcgaatata tccggcacat
     9181 cttgaacgaa accgaacgcc gcaatatccg ccagcacggc tacggagtcc gcagctttcg
     9241 ccggtcactc gaagagacgt acctgaaatt ggcgggtaat tcggcgcgac gcgagatcgt
     9301 gaaagaaatc gagcgagtgg cgctggagct ggaaagcacg cctccccata tcatggatgg
     9361 agtccccgaa acccttgtct acctttcgaa gcaccatcga ttgatactgt tgaccaaagg
     9421 cgagcctgct gaacaagccg ccaaagtgga gcgatccggg ttgcaatctt attttgaggc
     9481 gatcgaaatc gtactggaga aggattcagg cacgtacggc cggatcattg atcaatttaa
     9541 gatcgtcaag tcacatggct ggatggtcgg gaacagcccg cgcagcgaca tcaatcccgc
     9601 attgcaatca ggcctgaatg ccgtattcat ccctcattcc gctacttggg agctggaaaa
     9661 atcggaggtc gagagcggca cggggaaact gctgatcctt tccgcattcc gggacctgcg
     9721 agggcatttt tagcggccgt cacatctgcc gtccaatttg tcccgtgcgc ccggaacttc
     9781 cccactttcc gtcaaccccc ttactcgata ggatcagtaa gggagggtgc cgatttgata
     9841 gccgattatc gccacgaatt tgccgatttt gaagacgtca catatctaaa tgcggctctt
     9901 caaggcccga tgccgcttgt ggcagctcac gcggcggaag ttgcgctcga atggaaaaag
     9961 aggcccgacc gattgccgga ttccgcttac ttcgatctgc ccgaccgcat acgagaaaaa
    10021 acggccgcgc tgatcgggtc ccgagctgat gaaatcgccg tgaccaccgg ggcgagcgcg
    10081 ggtctcgcct gtgtcgcggc cggcattgat tggaagccgg acgacgaagt gctggtcgga
    10141 cagggcgagt ttccggcgca tttctcaact tggctacgct atcaaaaggc cgggaaactc
    10201 cgagtgcgcg tcatcactcc gaagggccgc ttcatcacag ctgacgatta tattgagcac
    10261 atcgggccgc agacacgcgt tgtttcggcg agcctggtgc ggttcgataa tggtgcgagg
    10321 ctggacgcgc gtcgcgtcgc gcaagcgtgc gagaaatacg gcgccgcgct tcttctcgat
    10381 gtatctcagt gcgccggagc tatgaagctg gatattcttg acttgggcgc ggccatggcg
    10441 gtctgcagcg gctataaatg gctccttggt ccgtatggca ccggattttt ctgggttgcg
    10501 aatgaatgga ttgaacgttt gacgctgggc tcggtctact tcatggcact cgaaggtgcg
    10561 cgaaccttcc acgcgctgcc gatcgaaaat caacagcccg ctcctggggt ccgccgctgg
    10621 gactccgcgg agacagcgaa tttcacgaat ctggccgcgc tcgattcgtc gctggatttt
    10681 cttttgcgca tcggcaccga tcggatcgct gctcacaccc gctcgcttgt ggatgagttg
    10741 atcgagcgtc tacccggcac gcggtgcaag cttgcttctc cggaggaatg cgatcgacgc
    10801 ggaccctatg cgtgtatttc cgcgtccact cccagcgata cgtcggcact ttacgagaaa
    10861 ctgcgcggcg cgcaagtgca tgtcagcttg cgcgagaacg cgctgcgcat cgcgccgtac
    10921 atctacaata cgcgcgagca aatttcgcaa ctgcttgaaa ttttatcgga gtagctggcc
    10981 ttcgaaagct attgagagtt gtaatctcgc ggattctgat acaggaaagg gatgtgcacg
    11041 acggcctcga cgtctactgg atggctggcg cgcgtcgccg ggcgaaactt ccattcgctc
    11101 agcgcctgga ttgcattttg gtccagtttt gggtcgagcc cgcgaacaac ttgaatgcta
    11161 tccactgaac cgtccttgcg aatgattgca taaagaacta cttcgccttg cacgtgcgcc
    11221 ttgatcatgt cgggcggata cttcggatcg gccttccgga tggggaccgg cccggcgagg
    11281 cgctccctcg gcgcgactga cggccggtct tcgtccaatt ccgaaaagct taggatccag
    11341 cttccgctgg cgctcgtgag attcggcaga ttcacgtgca tcgtgtacac ctcgctgccg
    11401 gagagaattt tttccggcgg caagctcgga tcgaggccgc tcacatctgg cggtcctcgt
    11461 cgagaattgc tgggcggctg ataagaattc ggcggattca gattcaaagt gttcaaacgg
    11521 ctggctgctg atggggcgat gcctccccga ctggatggtt tgccgcctcc agtaatactg
    11581 atcgctgccg ggagcgaatc tgaattggat ccggcacccg taccgccagt cgccgaaggc
    11641 gcggcagcgc caccggggcc cggtcttgtc gaatgatttg ccgcatcggc aacagctccc
    11701 ggctttttcc catctggcga agtcaaaact cgcgcggcca aatttccctc aggtactcca
    11761 gccgctggcg cgggcggaga tggtgtggcg gacaacgcga tgatgcgacg gctgctgctg
    11821 gtatcggcag cgccgatttc cggaacggcc gcggtatccg cgcgggtttc gcgctgccgc
    11881 gtggcgggcg ccgacattgc gctcacaggc attttggggc gcgggattgc gttggccttt
    11941 gaaacgatat tgaaaggaac tagattcttg gcgggattcg gaacttccgg cgcggcatca
    12001 tcccgaacag tgcggcgccg aattttcggc gctgagttcg caacggacaa ttggaacttc
    12061 ggtttctgcg gctgatcact agaccactgt acgatattag gcagttgcgg caccaccttc
    12121 ggaggggcgc tcggcgattc aggtcgtatc agagcttgtc tgggatgcgt tacgcgcacg
    12181 ggaatcgaaa ggatggtctg ccgcgcgcgg gaagcatccg cgcccgaact ttccggaaca
    12241 ctcggggcga tatttttcgg tttctcgcga ggggagggga cggcagcggg caacgaaatt
    12301 cgcggcagat caggctgttc gccggtccac gttagttcga tttgcaccgg agcaagcgtg
    12361 ggagtgtgct cgggcaaccc cccccaaatg ggcaggatca acagccaaat caccgcaacg
    12421 tgccacaaag atgacgcggc gaaagctttg ctcggaactc tgccgcgaat ccaatccaca
    12481 tggaagccgc gctcttttgt aagttcggcc tctttcggcg c
//
LOCUS       DATNDT010000109         6959 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2630902, whole genome shotgun sequence.
ACCESSION   DATNDT010000109 DATNDT010000000
VERSION     DATNDT010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6959
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2630902"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            342..3188
                     /locus_tag="VN788_03435"
     CDS             342..3188
                     /locus_tag="VN788_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HXU19619.1"
                     /translation="MDFVKEAAPDTVEAARGLIPAEELPSAANALAELFPEDPAPQTD
                     EPQSVGTPINISRDSAPRPKRRNIFLRLFSPKQSPAAPNESGATLHHNGNGKSKPTAT
                     DSFTPETGAVNNPPAPHDGTETANIEPEAAPIAASELPSAVQALAELFPPEATQTEVA
                     ESVSGDSVGLLDASDIEAALESLSPSEPAPIEASTDAVIPSTAELIEPSASVAAESEA
                     IASKPPVENLQAEPPEEKSIAQDENCPDELLSPAESSTEDLHAQVASGPVQDNPAEKF
                     DEENTPQSEEKAAESSEGSARRRPYKNWAFDDKLASHKEWIDSRGLSGKRADLAGADL
                     EAADLISVNLRLADLHDANLRAADLLLADLRDACLVRADLEDACLVGANLEAANLEGA
                     SLETAMGLVPRQIAGANLRDALLAPELMEFQAESTFNHSARRARAYFNIVTGASILSW
                     LMIWRTKDIQLVTDSSIFPFLHSHAAAALPTAELYLIFPVALLTLYVLLHFHLLRMWD
                     SVHELPAIFPDGRTLGDDQPGVITGLLRAHFRWMNPEPTASNMTERRLWQLAAYWVTP
                     LTLLLFWARYLTRQEIHGTLLHALLTAIATGIAFYAATRVGRPQEKWAVEWHQAQRVV
                     EKIKAINPMKLTGVLLFILILFSAGTIDGVPHERSRAPQYSIASIRRWAPTVFWSMGF
                     DPYADLTEASISRRPPESAIADDQLASIEGARLNNFRVRYAQAYAAFLAGAHLWQADL
                     QGAFLSEADLRGADLGQSNLRYALMDQARLYHANLDRSHLDGADLRRADLHESNLSYC
                     SFTNAILADARLDGANLYTSNLNFATLTRANIQKADLRNASLSNAHMDHADLRGAYLW
                     SSRLLGADLGGAQLGNAIFIDANLEGANLGGAQFSGTVLTGANLKGASLEGADLRGAS
                     GLTAGQVCSAKSRRGALLNDDLQMQVDAQCGRAQ"
     gene            3303..3491
                     /locus_tag="VN788_03440"
     CDS             3303..3491
                     /locus_tag="VN788_03440"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HXU19620.1"
                     /translation="MARYGKASQKRVKSAMHRRKKGTLRSGKGGKGGKVKSRKQAIAI
                     GLSEARKKGAKVPRKKSS"
     gene            3645..3986
                     /locus_tag="VN788_03445"
     CDS             3645..3986
                     /locus_tag="VN788_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19621.1"
                     /translation="MLQYLLRFVVGGLIVSAFAAVGGIVKPKSFAGLFGAAPSVALAT
                     LGLTIHASGKQFAATEGRSMIAGALAFCLFGYVARLLLAKGRFGAATVSIAGLGIWLI
                     AAIGAWLLVLK"
     gene            3993..4421
                     /locus_tag="VN788_03450"
     CDS             3993..4421
                     /locus_tag="VN788_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011090440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HXU19622.1"
                     /translation="MRVYFDASVFRRTTWDEYASRFLFGGAITLIAGILAKRYGPVFG
                     GLFLAFPAIFPSSVTLIEKHERQKKQRAGIPRTIRGQLAAALDARGAAMGSIGLACFG
                     LVVWKLLPIWSPALVLLVASVIWFAVSFLIWRARKFRLTR"
     gene            complement(4425..6107)
                     /locus_tag="VN788_03455"
     CDS             complement(4425..6107)
                     /locus_tag="VN788_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU19623.1"
                     /translation="MRRWPFKLSLFIAALTFVTALFSIQAFAQSNEAPAASARPAAAQ
                     NSETIVATPVVSDQLTNRCTAVRKLSSKTPAWTGWGANPPNWRYQNAMQAGFSVASVP
                     QLKLKWAFGIPNVKLVRSQPAIYGGRVYVGGNDGTVYSLDAATGCTYWATTEPKAVRS
                     GLLIGKAGSADAVFFGDAGGAVIALDAASGKFLWRTQADRHPAATITGTPSYFDGKLY
                     VPVSSGEEQARRRPGYECCTFRGSVVALDAATGKMIWQTYMAAETPVPHGKTADGRTI
                     MGPSGMAIWSAPTIDAAEKRIYVGTGDNYSEPDTKTSDAVVALDLETGKILWSAQFDK
                     EDVYKIECGNPPAAGCAQPGVPEFDLGASPILVMLPRRKRVLILGQKTGMVYGVNPDA
                     QGKLLWHQRAGTGGLLGGVQWGPATDGTNVFVAVSDLAFQDRGPDPSKGGGISAYQVS
                     NGKLLWKTPPPGCGERRPCSPAQSQAVTAIKGAVFSGSIDGHLRAYSAADGKIIWDFD
                     TARSFDTVNGVAAKGGSLDVGGPVIANGMMFVVSGYPGFGAMPGNVLLAFGR"
     gene            6357..6665
                     /locus_tag="VN788_03460"
     CDS             6357..6665
                     /locus_tag="VN788_03460"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HXU19624.1"
                     /translation="MPARAALNLSSRKGFAIVTNLVVPKTGGMNTTKVTVVKWLKNEG
                     DALKQGEPVVELETEKVSYELDAPIAGTLLKIITREPAEIDVGGLLGYIGQPGDRVPA
                     "
     gene            6695..>6959
                     /locus_tag="VN788_03465"
     CDS             6695..>6959
                     /locus_tag="VN788_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXU19625.1"
                     /translation="MAVQNGLGAQNGLGKDRLIWMYTQMCRIREFEERVKRTFVEYPG
                     TIRGHAHLGDGAEASIVGALATRHENDLVMPSYRCHGYPLVLGT"
BASE COUNT         1432 a         2136 c         1958 g         1433 t
ORIGIN      
        1 gtgcaatacg aatcccggaa ttcacgccag cgctaaaccg aagcaaatca ccctcttatg
       61 acagccataa ttgcgactcg ccatactaca ttcccaccta tcgcgtgtaa acccagattt
      121 gcccggacgc tttatcgccg ctagtccccg ctgcctatca gttccgatgc aaacgggggg
      181 aaccgaacgg acagctgtac tgctattgca ctcttggaca gcagcctccc gaaggctccg
      241 cacgatatcc taggcctgag taaactagac gttccggatc ggggaatccg gtcagttcta
      301 ctctgcccaa aatcgctcgc gaaacgctaa tcgggggttg tgtggatttc gtcaaagagg
      361 cggcgccaga tacagttgag gcagcacggg gactcattcc agcggaagaa ttgccctctg
      421 cggcaaacgc gctggcggag ctgtttccgg aggatcccgc gccgcaaacc gacgagccgc
      481 agagtgtcgg aacccccatc aacatttcac gtgattcggc tcctcgaccc aagcgccgaa
      541 atatattctt gcggctcttt tctcccaagc aatcgccagc cgccccgaat gaatctggag
      601 cgacgctcca ccataacggg aacgggaagt ccaaaccaac tgcaacggat tcgttcacac
      661 cggaaacggg tgcggtaaac aatcctcctg cacctcacga tggcaccgaa accgcgaaca
      721 tagaaccgga ggcggcgcca atcgcagcca gcgaacttcc ttccgcggtg caagcattgg
      781 cggaattatt cccgcccgaa gcaacgcaaa cagaagtcgc ggaatccgtc tccggcgatt
      841 ccgtgggcct ccttgacgcc tctgacatag aagcagccct ggaatctttg tctccgagtg
      901 aacccgctcc catcgaagcg agcaccgatg cggtgattcc gtccaccgcg gagcttatcg
      961 agccttcagc ctccgtcgcg gcagaatcgg aagcaatcgc atcgaagcca cccgtcgaga
     1021 atttacaggc tgaaccgccg gaagaaaaat caatcgcaca ggacgaaaat tgcccggacg
     1081 agcttctgag tccagctgaa agttcaactg aagatttgca tgcacaggtt gctagcggcc
     1141 ctgtgcaaga caatcccgct gaaaagtttg atgaagaaaa tacgccgcaa tctgaggaaa
     1201 aagccgcgga atcctccgag ggttccgcga ggcgcagacc ttacaaaaat tgggctttcg
     1261 acgacaagct ggcgagccat aaggaatgga ttgattcgcg cgggctcagt ggcaagagag
     1321 ccgatctggc cggcgcggac ctcgaagcag cggacttgat cagcgtgaat ttgcgcctcg
     1381 ccgatttgca tgacgccaat ttacgcgccg ctgacctttt gctggcggac ctgcgcgacg
     1441 cgtgcctcgt tcgcgccgat ctggaagacg catgtctcgt cggtgcaaat ttggaagccg
     1501 caaatctaga gggcgcttcg ctggaaacgg ccatggggct ggtgcctcgc caaatcgcgg
     1561 gagcgaacct gcgcgatgcc cttctcgctc cggaactgat ggagttccag gcggagagca
     1621 ctttcaatca ctcggcacgg cgcgcgcgcg cgtacttcaa tatcgtcacc ggcgccagta
     1681 ttctctcctg gttgatgatc tggaggacga aagatattca gctcgtgacg gattcttcca
     1741 tatttccatt cttgcattcc cacgcggccg ctgcgttgcc gacggccgaa ttgtatttga
     1801 tttttcccgt ggcgctcctc acgctgtacg tcctgctgca ctttcatttg ctgcgaatgt
     1861 gggattccgt ccatgagctg ccggcaattt tccccgatgg gcggaccctg ggcgacgatc
     1921 agcccggcgt catcaccggc cttctgcggg cgcactttcg gtggatgaat cccgagccta
     1981 ccgcgtcgaa tatgacggag cgtcgcctct ggcaattggc cgcctactgg gttactccgc
     2041 tcacgctgct gctattctgg gctcgctatc taacgcgcca ggaaattcac gggacacttc
     2101 tgcatgccct cctcacggcc atcgcgaccg gcatcgcgtt ttacgccgcc accagagtcg
     2161 ggcgcccaca agaaaaatgg gcggtcgagt ggcatcaggc gcagcgtgtc gtcgaaaaaa
     2221 tcaaagccat caatccgatg aagctgaccg gcgttctgct attcattttg atccttttct
     2281 ccgccggcac gattgatggt gtgcctcacg aacgcagccg cgcccctcaa tacagcatcg
     2341 cgagtattcg ccgctgggcg cccactgtct tctggtcgat gggttttgac ccttacgccg
     2401 atctcaccga ggcgtcgatt tctaggcggc cgcccgagtc cgcgattgcg gatgaccagc
     2461 ttgcttctat tgagggagcc cgattgaaca atttcagagt gcgctatgcg caagcctacg
     2521 cagcgttttt ggccggcgcc cacctctggc aggcggacct tcaaggcgcg ttcttgtccg
     2581 aggccgattt gcgcggcgcc gacctcggac agagcaactt gcgctatgcg ctgatggacc
     2641 aggcgcgcct gtatcatgcg aatttggatc gttcgcatct cgatggcgcc gacctccgtc
     2701 gcgccgattt gcacgagtcg aatctatctt actgttcgtt tacgaacgca attctggccg
     2761 acgcccgctt ggatggggcg aatctctata catcgaatct caacttcgca acgctaacgc
     2821 gagccaacat tcagaaggca gacctgcgca acgcgtctct ctccaacgct cacatggacc
     2881 acgccgacct gcggggcgca tacctttggt cgtcccgatt gctgggtgcg gatttgggcg
     2941 gggcccagct cgggaatgcc attttcattg acgcaaacct cgaaggcgcg aatctcggtg
     3001 gagcgcagtt ctccggcacc gtgttgaccg gtgccaacct caagggtgcc agcctggaag
     3061 gcgcggacct tcgcggtgcc tcgggactga ccgctggcca agtctgctcg gcaaagtccc
     3121 ggcgcggcgc cctgctgaac gacgacttgc agatgcaggt tgacgcccaa tgcggccgcg
     3181 ctcagtagtc atacctaaca cgtctctttt ttccgatccc cgaagattca cctggaaaat
     3241 attcgcgagg ctggcgtcac tccggcgctg aattttcatc tacctttaca ggaggattgt
     3301 tcatggccag atacggcaag gcttcgcaaa agagggtcaa gagcgccatg caccggcgaa
     3361 agaagggaac tctgcgctcc ggcaaaggcg gaaaaggtgg caaagtcaaa agtcgtaagc
     3421 aagcgatcgc cattggtctc tcggaggccc gaaagaaagg cgccaaggtc ccgagaaaaa
     3481 aatcgagctg agcgcgggac tcgacagctc gcgtcgcgca tccgctttaa ctccacgctg
     3541 cgcttcgttt tgctcgccgg cgcccgaggc tcgactaggc gtgagacctg cgcgccagaa
     3601 gggaagcctg tcccttcccg cttgacagcg gagagcgacg taagatgctc caataccttc
     3661 ttcggtttgt ggtgggcgga cttattgtct cggcatttgc agcggtaggc ggcatcgtaa
     3721 aaccaaaaag cttcgctgga ttgttcggcg ccgctccttc ggtggctctg gcaacacttg
     3781 gattgacgat ccatgccagc gggaagcaat ttgccgccac cgagggccga tccatgattg
     3841 ccggagccct cgccttttgt ctctttggct acgtagcgcg ccttctgttg gccaaggggc
     3901 gtttcggtgc cgcgacagtt agcatcgccg gtctcggcat ttggttgatc gccgccatcg
     3961 gcgcgtggct gctggtgctg aagtaacagg ccatgcgagt ttatttcgac gcatccgtgt
     4021 ttcgcaggac gacgtgggac gaatatgcct cgcgcttctt gtttggcggc gcaattaccc
     4081 tgatcgcggg cattctggcg aaacgatacg ggcctgtctt cggcggcctt ttcctggctt
     4141 tccccgcgat tttcccctcc agcgttactc tcatcgaaaa acacgagcgc caaaagaagc
     4201 agcgcgcggg aataccgcga accattcgcg gccagctcgc ggcggcgctg gacgctcgcg
     4261 gcgcagcgat gggtagcatc ggattggcgt gctttggcct ggtggtctgg aaactgctgc
     4321 cgatttggag tcccgcgttg gtcctactcg tcgcgtcagt gatatggttt gcggtttcat
     4381 ttctcatttg gcgcgcacga aaattccgcc ttacgcgcta ggccctagcg gccgaacgcc
     4441 agcagcacat tgccaggcat tgcgccaaac ccaggatagc cggacaccac gaacatcatt
     4501 ccgttcgcaa tcaccggtcc gccgacatcc agcgaaccgc ctttcgcggc cacgccattc
     4561 acggtgtcaa atgaacgcgc ggtatcgaaa tcccaaataa tctttccgtc tgccgcggaa
     4621 tatgcgcgca aatgtccatc gattgagcct gagaacaccg cgcccttgat cgccgtcacc
     4681 gcctgggatt gcgcggggct gcacgggcgt ctttcgccac aacccggtgg cggcgttttc
     4741 caaagcagtt tgccgttgct cacctggtaa gccgatatgc cgccgccttt cgacggatcg
     4801 ggcccgcggt cttgaaaagc caggtccgac accgccacga acacatttgt gccgtccgtg
     4861 gccggacccc actggactcc gcccagcagc ccgccggtcc ccgcgcgttg atgccagagc
     4921 agtttcccct gcgcgtcggg attaacgcca taaaccattc cggtcttctg ccccaatatc
     4981 agaacgcgtt tcctgcgcgg cagcattacc agaatgggtg acgcgcccag atcaaattcg
     5041 ggaacgccgg gttgcgcgca gccggctgca ggaggattgc cgcattcgat tttgtaaacg
     5101 tcttctttgt cgaattgcgc cgaccacaga attttgccgg tttcgagatc gagcgcaacc
     5161 accgcgtcgc tcgtttttgt gtctggctcg gagtagttgt ctccggttcc cacatagatt
     5221 ctcttctcag ctgcatcaat cgtcggcgcg gaccaaatcg ccatcccgga aggccccata
     5281 atcgtgcgcc catccgctgt tttgccgtgc ggcaccggag tttccgctgc catatacgtc
     5341 tgccaaatca tctttccggt cgccgcgtcg agcgcaacga cgctgccgcg aaacgtgcaa
     5401 cactcatagc cggggcgtcg ccgtgcctgt tcctcgcccg acgataccgg cacgtacaac
     5461 ttcccgtcga agtagctcgg cgttccggtg atcgtcgcgg ccggatggcg atccgcctgg
     5521 gtgcgccaca gaaattttcc ggaagcagcg tcgagcgcga tcaccgcccc gcccgcgtcg
     5581 ccgaagaaaa ctgcatccgc cgaacctgcc tttccgatca gcaggccgga gcgaaccgcc
     5641 ttgggttcgg tcgtcgccca gtacgtgcaa cccgtcgcag catcgagcga gtagacagtg
     5701 ccgtcgttcc cgcccacata tacgcggccg ccataaatcg ccggctgaga tcgcacgagc
     5761 ttcacattgg ggattccaaa cgcccacttg agtttcagtt gcggcacgct cgcgacggaa
     5821 aagcccgcct gcattgcgtt ctgatagcgc caatttggcg gattcgcgcc ccagcccgtc
     5881 cacgccggag ttttcgagga cagtttgcgg accgccgtac accgattcgt aagctgatcg
     5941 gagaccaccg gcgtggcgac tatcgtttcc gaattttgtg ccgccgcagg ccgcgcactt
     6001 gccgcaggtg cctcattcga ttgagcgaaa gcctgaatac tgaaaagcgc ggttacaaag
     6061 gtcaaagcgg caataaaaag ggaaagctta aaaggccagc gtctcattat ttctcccgaa
     6121 cgtaagcggc aatcgcagat ttacacagtc gcactacaat tgcggagcac gagagaattg
     6181 tactgaactt cttcgcgacc tcgctcgatt ttaggccgct ccatatccga tggattacgc
     6241 cgagtcgcac gctaacgtcg cttggtccgt ccggtccgtt aggttgacat cctgactgaa
     6301 actgcgctaa taaagccgtt gcggtgggcg tccttcgcct atccatgtcc ggatgattgc
     6361 cagctcgcgc cgctttgaac ctcagctcca ggaagggatt tgccatcgtc accaatctgg
     6421 tagtgcccaa aacaggcggc atgaacacga ccaaagtaac cgtggtcaag tggctcaaga
     6481 atgaaggtga cgccctcaag caaggcgagc cggtcgttga gctggaaacc gaaaaagtga
     6541 gttatgaatt ggacgccccg attgcaggca ccctgctcaa aattattact cgcgagccgg
     6601 cggaaataga tgtcggcggg ctgctaggct acatcggcca gccgggtgac agggtccccg
     6661 cataggaatc gctggaagtt cggaggaatt caccatggcc gtacagaatg gactcggcgc
     6721 gcagaatggg cttgggaaag atcggctgat ttggatgtac acccaaatgt gccgcatccg
     6781 cgaattcgaa gaacgcgtga agcgcacctt cgtggagtat cccggcacga ttcgcggcca
     6841 cgcgcatctc ggcgacggcg cggaagccag cattgttggc gcgctggcca cacgccacga
     6901 aaacgatctg gtaatgccca gctatcgctg ccacggttac ccgctggttc ttggcacgc
//
LOCUS       DATNDT010000110         4979 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4077687, whole genome shotgun sequence.
ACCESSION   DATNDT010000110 DATNDT010000000
VERSION     DATNDT010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4979)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4979)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4979
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4077687"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..785)
                     /locus_tag="VN788_03470"
     CDS             complement(<1..785)
                     /locus_tag="VN788_03470"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HXU19626.1"
                     /translation="MSTATRNSYLLESFADVDAFAEIPYSRLTDMQPSHKPHALRPGD
                     VVRVLSLASPVQEDRLQKGYDEIKRLGYTPQMEAPAARQEGSFFADSAENRLRALQGA
                     LQDSGTRAIFCSRGGYGSNYLIEGLGAAATAPKIFLGSSDITSLQAYLWQKLNWVTFY
                     GPMVASNFDQGAGAGNGYDEASLIRALTATEEGWMLDLQGEPLIKGSARGIAVGGCLT
                     LLETTLGTPWEIETNGAILVLEDRGMKPYQVDRALMHMKQAGK"
     gene            complement(782..1717)
                     /locus_tag="VN788_03475"
     CDS             complement(782..1717)
                     /locus_tag="VN788_03475"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXU19627.1"
                     /translation="MNGWRRAALASLAGVLVFAGCGGGSSLTATNATPVPQSMVPSNI
                     TAGSDGFAVFITGSGFVSSPKGVTFAYWNGAPRSTTFNLTTNQLTVQILASDVAATGV
                     GSITLVNPPPGGGPSQNGLDFTIVAAQPGGRTISSFSPASANAGDKAFTLTVNGDNFT
                     VNDVVTWNGSVRPTTFMSSTQVSAQINATDVADSGLASVAVNAPGLLIASPAVNFPIG
                     GPDNASPSISSLSPSSTSAGEEDLQIFVKGSGFATSSVVELDNVPLATSFLSASQLIA
                     VIPAADLAAKGMLQVAVTTPAPGGGLSKTLTFTVN"
     gene            complement(1896..2798)
                     /locus_tag="VN788_03480"
     CDS             complement(1896..2798)
                     /locus_tag="VN788_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXU19628.1"
                     /translation="MSYRAIILAALVICVSPQVAMTQAAGQSAAATPAAVSTSPDQAK
                     LLKSTEVFVRVLFGWGSDVKVDVGPLSPSAAADYYRVPVSVVLNGQKESGEVYVSKDG
                     KTLLRGEIFDMGTDPFAANRAKIHVDGSPTKGPADASVTLVEFSDFECPHCRALWAAM
                     KAVETRYPQIRIVYKDFPLTNIHPWAQTAALGGRCAFEQSPAAFWKVEDAIFDNQDVL
                     SPENVWDKLVEFATGAGLNADTFKACLASPEAAKAVDASHAEGVALGVDSTPTVYING
                     RPVVGGDENTLVQYINFELAAAKK"
     gene            complement(2994..4424)
                     /locus_tag="VN788_03485"
     CDS             complement(2994..4424)
                     /locus_tag="VN788_03485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19629.1"
                     /translation="MLRPILIGLVLSILLCGSRRAGADVGLVLNDSLDTSVARITGSG
                     HSAIYFSRICPETPIKMRLCRSGENGSVVSNYTSLGEDQPFEWNIVPLDVYLYGVANP
                     ANRPLFASAKIKNLLEMEYRERTLRDYCASHSCQTSEKAEWREMVSATSERTIYIFIV
                     KTTRRQDLDLIAEFNAAPNVNRFNGFGRNCADFSKRIINTYFPHATHRDFLNDFGMTS
                     PKAVARTLARYAHHHPELQYRVVHFAQSPGTIKHSSECRSGTEQLYRSKKLLVPMAFF
                     AWHELPIAAGSYLLTGRFNPEREFERHASVRETSTEDELKIAESDYDKTAAASLKKVE
                     ARERTHAVGTPKEWAAYREEFDSMVSESASNGLIPGRESLNHVFRDLNERGKPFVDDR
                     GGLWMEVDEGGETVKVGLSPSNVLAPGSNRPLAYQMILAHIDDVLKSSPRRRETIPEF
                     QQTWGMLQQVRAQLQSGKGPALAMKR"
     gene            complement(4800..>4979)
                     /locus_tag="VN788_03490"
     CDS             complement(4800..>4979)
                     /locus_tag="VN788_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009262250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19630.1"
                     /translation="GKLHGAKIDPQGDHRIAMALSVAALGAEGGTTIRDAECVGVSFP
                     EFFPTLDRLRGGEAS"
BASE COUNT         1056 a         1491 c         1369 g         1063 t
ORIGIN      
        1 aactttccgg cctgcttcat gtgcatcagc gcgcggtcaa cctgataagg tttcatcccg
       61 cgatcttcga gcaccaaaat tgcgccgttc gtctcgattt cccacggtgt gccgagagtc
      121 gtctcgagca gcgtcaggca gccgccaact gcgattcctc gcgccgaacc tttgatcaac
      181 ggctcgcctt gcaaatcgag catccagcct tcctcggtag ccgttaacgc gcgaatcagg
      241 ctcgcttcat cgtagccgtt ccccgctccc gcgccttgat cgaagttcga cgcgaccatc
      301 gggccataaa acgtcaccca attcagcttt tgccacaagt acgcctgcag cgatgtgata
      361 tcgctcgatc caagaaaaat cttcggagcc gtcgccgccg cgccgaggcc ttcaatcaga
      421 tagttggagc cgtaaccgcc gcgcgaacaa aaaattgctc gcgtgcccga atcttgcaac
      481 gcgccttgca aagcgcggag acgattctcc gccgaatctg caaaaaagga cccttcttgc
      541 cgcgcagccg gagcctccat ttgtggcgta tagcctagcc gcttaatttc gtcgtagccc
      601 ttctgcaagc gatcctcctg caccgggctg gctagtgaaa gcacgcggac cacgtcacct
      661 gggcgaagcg catgcggctt gtgcgacggc tgcatatcgg tcaatcgaga atagggaatt
      721 tccgcgaacg cgtctacatc cgcaaaggac tcgagcaggt aagagttacg agtagccgtg
      781 ctcaattgac tgtaaaggtc agagttttgg aaagaccgcc gcccggcgcc ggcgttgtta
      841 ctgccacctg taacataccc ttcgccgcaa gatccgcggc cggaatcact gcgatgagtt
      901 gcgaggcgct caagaaagaa gtagctagcg gtacgttgtc caattcgacc acggagctgg
      961 tcgcgaagcc cgagcctttc acgaaaatct gcaaatcctc ctctcccgcg gaagttgacg
     1021 aaggcgaaag cgacgaaatg ctcgggctcg cattgtccgg ccctccgatg ggaaagttga
     1081 cggcgggaga cgcgatcaac agcccgggcg cattcaccgc aacgctcgct aatccgctgt
     1141 ctgccacatc ggtagcgttg atctgcgcgc tcacctgtgt ggaactcatg aaggtcgtcg
     1201 gtcgcacgct tccgttccat gtcaccacgt cattcacggt gaaattatcc ccattcacgg
     1261 ttagtgtgaa cgccttgtcg ccggcattag cgctcgcagg agaaaacgaa ctgatcgttc
     1321 gcccgccggg ctgcgccgcc acgatcgtga aatccagccc attttgtgac ggtccaccgc
     1381 ctggaggcgg attcacgagt gtgatgctac caacacctgt cgcggcaacg tccgacgcca
     1441 ggatctgaac ggtcagttga ttggtggtca ggttgaaggt ggtggagcgc ggcgcgccat
     1501 tccaataggc aaaggtaacg ccctttggac tcgatacaaa gccggatccg gtgatgaaaa
     1561 ctgcaaagcc gtcgctcccg gccgtaatgt tcgaaggaac catggactgc ggaacgggcg
     1621 tagcgttcgt cgccgtgagg ctcgagcctc ctccgcaacc tgcgaacacc agcactcctg
     1681 ccagcgatgc aagcgcagcc cgccgccaac cgttcaagaa agcctcctca aaatttcaaa
     1741 cggatatccc gaagctaccg ccaccggcag cctggaaaac ggagcgagga aaaacacaac
     1801 acgcccggca aaacgaccaa agtgatagca cccccgcaag agccacgtca actcggattc
     1861 aggctcctgg gaaagggttg gcctcgggtg cgatttcact ttttagctgc ggcaagttca
     1921 aaattgatat attgcaccag cgtgttctca tcgcctccca ccaccggccg cccattgatg
     1981 tagaccgttg gcgtgctgtc cacaccaagc gccacgccct cggcgtgact cgcatcgacc
     2041 gcctttgccg cctcgggtga tgccaagcag gccttgaacg tgtcggcatt gagccccgcg
     2101 ccggtcgcaa actccaccag cttgtcccac acgttttcag gagagagcac gtcctgatta
     2161 tcaaaaatcg cgtcttccac tttccagaaa gccgccggag attgctcgaa ggcgcagcgt
     2221 ccgccgagcg ccgctgtttg cgcccaggga tgaatgttgg tcagaggaaa atccttgtac
     2281 acgattcgaa tctggggata gcgcgtttcc acggctttca tcgctgccca caacgcgcgg
     2341 caatgcgggc attcaaaatc gctgaactcg accagcgtca ccgaagcgtc agccggtccc
     2401 ttcgtgggac ttccgtccac gtgaatcttg gcgcgattgg cggcgaaagg atcggtcccc
     2461 atgtcaaaaa tttcgccccg cagtaaagtc ttgccgtcct tgctgacata aacttctccc
     2521 gattccttct ggccgttgag gaccaccgaa accggaaccc tatagtaatc cgccgccgcg
     2581 gacggcgata aggggcccac atcgaccttc acgtccgagc cccagccaaa aagcacgcgc
     2641 acaaacacct cggtcgattt gagcaacttc gcctggtccg gtgaggtaga gactgccgcg
     2701 ggcgtagcgg ctgctgattg gccggcggcc tgcgtcatgg caacttgcgg cgacacgcag
     2761 atcaccagcg ccgccaagat aatggctcga taagacacgc gaatcacctc cactgcgaat
     2821 ccatcttaga tttagatgag accaaccagc ataaagaaaa agtcttcgaa gcgcaaaaac
     2881 cgttcgcagg ctgcgaaaaa gcggcctcga atcacagtgg taaagtagtt cgaatcgtag
     2941 ggatcgaaca cacctgtctt gcggcagcgt gcggctggtc ggaatcgttg gcgctaccgc
     3001 ttcatagcca gcgcgggccc ttttccactt tggagttgcg ctcgcacctg ctgcagcatg
     3061 ccccatgttt gctggaattc gggaattgtc tcgcgtctgc ggggcgaact tttcagcacg
     3121 tcatcgatgt gcgcgagaat catctgatag gcaagcggcc ggttcgagcc tggcgccagc
     3181 acgttgctag ggctcaagcc aaccttgacc gtctcaccgc cttcatcaac ttccatccaa
     3241 aggccgccgc gatcgtctac gaagggttta cccctttcat ttaggtctct aaacacgtga
     3301 ttcagggatt cgcgccccgg tatcaggcca ttgctggccg attcgctaac catcgagtca
     3361 aattcctcgc gatatgccgc ccattccttc ggcgtgccca ctgcgtgcgt gcgttcccgc
     3421 gcttccacct ttttgaggct agcggccgcg gtcttgtcgt aatcggattc cgctattttt
     3481 agctcgtctt cggttgacgt ttctcgaaca gatgcatgcc gttcgaattc gcgctcgggg
     3541 ttgaatcgtc cggttagcaa gtacgatccc gcggcaatgg gcagctcgtg ccaggcgaag
     3601 aaagccatcg gaacgagcag ctttttcgaa cgatacagtt gttcggtacc cgaccggcat
     3661 tcgctgctgt gcttgatcgt ccccggcgac tgggcgaaat gaaccacgcg atactgaagc
     3721 tcgggatggt gatgggcgta tcgcgccagc gtgcgcgcaa ctgctttcgg gctggtcatt
     3781 ccgaaatcat tcaaaaagtc gcggtgagtg gcgtgtggaa aataggtgtt gatgatacgc
     3841 ttgctgaaat cggcgcaatt gcgaccgaaa ccgttaaaac gattcacgtt tggagcggca
     3901 ttgaattctg cgatcagatc gagatcctgc cgcctggttg tcttgacgat aaaaatgtaa
     3961 atagttcttt ccgaagtggc gcttaccatt tcccgccatt cggccttctc gctggtttgg
     4021 caagagtggc tggcgcagta atcgcgcagc gttctctcgc gatattccat ttccagcagg
     4081 tttttgattt ttgccgacgc aaaaagcggc cgatttgccg ggttcgcaac gccgtagaga
     4141 tacacatcca gcggcacaat gttccattcg aatggttgat cctcgcccag agacgtgtaa
     4201 ttgctgacga ccgaaccgtt ctctccggaa cggcaaagac gcattttgat tggtgtttca
     4261 gggcaaattc gggagaaata aatcgcgctg tggccggaac ctgtgatccg cgccacgctg
     4321 gtgtccagcg aatcgttgag gaccagaccc acgtccgccc ctgccctgcg ggaaccgcaa
     4381 agcaggatgg ataacaccag gccaattaaa attggtcgga gcacatctct ccctttcagt
     4441 gcgaatttgg cgggtctgtt cagtgaaacg catccctgcg gcaagcgtca agcagcagtc
     4501 accgccttgg ttccacttgc ggcacctcat tcctccttga ttttggatct gtaagttaga
     4561 tgcaagttat tggcacggag tcacttgcgc catccatgca ccaaatgtgg agtgcgggag
     4621 cttgtttacc ctgggtttcg aagggctccc gccgtttgcc gctcgggctt gctcgggcgt
     4681 gctccgaggc ggcgcagcga tcgcggctaa ttgacgaagt atgaggcatc cgctccctct
     4741 ttagtctttg agggtgcggc ttccgttcag actgtgaagg ttgcttctgc gtggattttc
     4801 taggaagctt caccgcctcg caaacggtcg agcgtgggaa aaaactcagg gaacgagact
     4861 ccgacgcact cggcatcgcg gatggtcgtg ccgccctcgg ctcccagggc cgccaccgac
     4921 agcgccatgg cgatgcggtg gtcgccctgt ggatcgattt tggctccgtg cagtttccc
//
LOCUS       DATNDT010000111         5328 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2053838, whole genome shotgun sequence.
ACCESSION   DATNDT010000111 DATNDT010000000
VERSION     DATNDT010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5328
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2053838"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VN788_03495"
     CDS             complement(<1..372)
                     /locus_tag="VN788_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19631.1"
                     /translation="MMMQKYLMRMAMILAVLLLAPSIAQRAAAQEAQQGQQGQQPSYT
                     LPEYNAEQAAAAEKDPQARIKKLDAFVAQFPNSTLMEYIYQFYYQTYYQLKQYPKAIE
                     YADKLIALDKANLALRVQALQA"
     gene            600..1328
                     /locus_tag="VN788_03500"
     CDS             600..1328
                     /locus_tag="VN788_03500"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU19632.1"
                     /translation="MGAIATNRLLEGRVALVTGASRGIGKAIARTLGRMGAKLSLCAR
                     DTQTLQSARRELEAEGSTVLDVPADISRPDDITSLVQATERSLGPIEILVNNAGIGHF
                     APIQECSDANWDATLDTNLRAVFLLSKAVAPGMISRRSGHIINIASLAGKSAFAGGSI
                     YCASKWGLLGLTQCAAEDLRPYGIRVSAICPGSVATDFSPHSGKDQGKMLQPDDVAHA
                     VEMILTQAPQSFISEVLLRPTQKP"
     gene            1392..2081
                     /locus_tag="VN788_03505"
     CDS             1392..2081
                     /locus_tag="VN788_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HXU19633.1"
                     /translation="MINIRLSEDRGHNQLPWLDSHFTFSFDRYYDPDHVQFRSLRVIN
                     EDVIAPAQGFPMHPHRDMEILTWILEGALEHRDSSGGSGVIRPGELQHMTAGSGVMHS
                     EFNPSPQKPAHLLQIWLLPERKGLAPSYEQLAFPDSDLRGKFNLVAGPKAPITIRQDA
                     NLYIGRLNEGETAKHAIASGRHAWLQIARGSVELNGRELKAGDGAAVSGESELTVKAR
                     EASEVLAFDLA"
     gene            2262..3452
                     /locus_tag="VN788_03510"
     CDS             2262..3452
                     /locus_tag="VN788_03510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014693007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXU19634.1"
                     /translation="MKFGGIHLMGLPRPWTSESEHALLNDALNLAELSDQCGFDYVWG
                     TEHHFLEEYSHSAAPEMFLAACSQRTKNVRLAHGIIQTPPNINHPARVAERIAVLDLI
                     SNGRCEFGMGSGATTTELGGFNVSQEEKKAMQIEGFRVAVRMLVENPFTGFEGQYVKV
                     PPRNVVPKPLQKPHPPLWMACSRRESILEAARLGLGALTFSFANPSEARQWVKDYYET
                     IENECEPLGYAVNPNVAIAAPFLCDRDQTRAEEIGAENYGFFVYGLGHYSFFGEHRPG
                     KTDIWKGYKTNPKEAARPEFTANHCIGTPKKLRETLREFEEAGVDQVVAVRQIGHLSL
                     DIQSSSTQLFSEEVLPEFKERELANARRQAERNARISEKAMARKPKVEAPIVETVIQS
                     AGRH"
     gene            3643..4380
                     /locus_tag="VN788_03515"
     CDS             3643..4380
                     /locus_tag="VN788_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009950247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
                     /protein_id="HXU19635.1"
                     /translation="MTVKTVVDGTFRQTNLTNESPEYLAKREELRLAEIELTRQRERV
                     ADLRRHLPKGAAIQDYEFEEGPRDLNAGDEPVRNIQLSELFTKPNRSLVIYHLMYGKR
                     QTKACPMCTAWLDAANGIAHHLAQNLDFAVVAAADIPTLRAHARARGWDKLRLLSAGN
                     NTFKYDFGSENREGQQDSTISVFTRDADGTVRHFYSAHPWLAPDVLERGIDLLNPMWH
                     FMDLTPQGRGDWYASLNYGTKVQAAST"
     gene            complement(4445..>5328)
                     /locus_tag="VN788_03520"
     CDS             complement(4445..>5328)
                     /locus_tag="VN788_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19636.1"
                     /translation="DMAKFTIAPHMRLHEWVAEEKGYFAAEGLDYEFREALASDEAKK
                     HNLGDKVGAFQTFEKGRGSDIGCACHWTVNVSASVGHGKLYADAYSVAPAGIFVPPES
                     PIHDPAELAGVPISVGYQSGSHYSAIQSLEQYMKPADIKLSFADGMLFSRMELLIDRK
                     VPAASLFSGPYYLLEQLGFRKVIDTTFMMASLITRDPDMDDVRKYFRALRKAQRDIDL
                     RPDLYTHYYKKEFPTRFHDVMDTRRWGPGERLVFEPYTKDVFKQSFDWISEHGIFPEG
                     AMGSGSYEDSCVTLASK"
BASE COUNT         1209 a         1512 c         1541 g         1066 t
ORIGIN      
        1 agcctggagc gcttgcactc gaagagccaa gttcgcttta tcgagggcga tcagcttatc
       61 ggcatactcg atggccttgg gatactgctt cagctggtaa taggtctggt agtaaaactg
      121 atagatgtac tccatcagcg tcgagttagg aaactgcgcg acgaacgcgt cgagcttttt
      181 gattcgtgct tgcggatcct tctccgccgc tgccgcttgc tcggcattgt actcgggcaa
      241 ggtgtagcta ggttgctggc cctgttgacc ctgctgggcc tcttgggccg cagcccgctg
      301 cgcgatggaa ggcgccagga gaagaacggc caaaatcatt gccatcctca tcagatactt
      361 ttgcatcatc actcttcgcc tcctgggctg aaactcattt cgggctgcca cggcggcata
      421 gagatcaccg ccgctgctgc gaggcaaaaa ccgcgcaagc aactgaaatc cacgggatta
      481 tagggaaagc cttccacgca cgcaagtctt aaaccagtaa agccttcgat gggtgttttc
      541 ccgtgccacc ttgtccacat gttaggatgt aattgagacg gcagaagtgg cctttgcgaa
      601 tgggtgccat agcaacgaat cgtttgctcg aaggcagggt cgctctggtt acgggcgcga
      661 gccgtggtat cggcaaggcc attgcgcgga cccttgggcg catgggcgcg aagctcagcc
      721 tgtgcgcgcg agacacccaa acacttcaaa gcgcgcgccg cgagcttgag gccgaggggt
      781 ccacggttct ggacgtgcca gcagacatct ctcgccctga tgacatcaca tcgctcgtgc
      841 aagccacgga gcgttccctt ggtcctatcg agattcttgt gaataacgca ggaattggcc
      901 atttcgcccc gatccaagaa tgttccgacg cgaattggga cgctacattg gacactaacc
      961 ttagggcggt tttcttgctt agcaaagcag tggctccggg aatgatttct cgccgcagcg
     1021 gccacatcat caatatagcg tcgctcgcgg gcaaaagtgc gtttgccggc ggcagtattt
     1081 actgcgcgtc gaaatggggc ctgttgggac tcacccagtg tgcagccgag gatctccgtc
     1141 cttatggcat aagggttagc gcgatctgtc ccggttccgt ggccacagat ttttctcctc
     1201 actcgggcaa agaccagggc aaaatgcttc agcccgatga cgtggcccat gccgtggaga
     1261 tgatcctcac ccaagcgcca cagagcttta tcagcgaagt gcttctgcga cccacacaaa
     1321 agccctgatc cgccgccgaa tccttgcaga cctgtgcggc atcaaaccac ttccaaagca
     1381 gaggcaactc tatgattaac atccgacttt ccgaggatcg cggccataac cagcttccgt
     1441 ggctcgattc gcacttcaca ttttcgttcg accgctacta cgaccccgat cacgtgcaat
     1501 tccgttcgct gcgcgttatc aatgaggacg taatcgcgcc cgcccagggt ttccccatgc
     1561 atccacaccg cgacatggaa attctcacct ggattcttga aggcgccctg gaacatcggg
     1621 atagctcggg gggcagcggc gtgattcgcc ccggcgaact ccagcacatg accgcaggca
     1681 gcggcgtgat gcacagtgag ttcaaccctt cgccccaaaa accagctcac ctgcttcaaa
     1741 tctggcttct tccggagcgc aagggcctgg caccgtcgta cgagcagctc gcatttccgg
     1801 actcggactt gcgcggcaag ttcaatctcg tcgccggacc gaaggcccca atcacgattc
     1861 ggcaggacgc gaatctttat atcgggagat taaacgaagg cgagaccgca aaacacgcca
     1921 tcgcatcggg ccgccacgcg tggctacaaa tcgcgcgcgg gtctgtggaa ctcaatggac
     1981 gcgaattgaa agccggcgac ggcgcggcgg tttccggcga gtctgaactc acggtgaaag
     2041 ctcgagaagc ttcggaagtc ctcgcgttcg atctcgcttg aacctgaggc ggtctcccag
     2101 gtgccattcg gccgtgcgga gctgcttgtt cgaacccatc taaaaaattc tcgcagactc
     2161 agcacgatcc aaaaattcgc gcgagcagcg cggatgcgga tgtttgcgcg atttaggcgt
     2221 tggcgtagaa tacgctgcgc actcgcgcga caggaattga aatgaaattt ggcggcatac
     2281 atctgatggg acttccgcgg ccttggacga gcgagtccga gcacgcgctg ctgaacgacg
     2341 ctctcaacct ggcggaactt tccgaccagt gcggattcga ttacgtctgg gggacggagc
     2401 accatttcct cgaggagtat tcgcactccg cggccccgga aatgttcctc gcagcctgca
     2461 gccagcgaac gaaaaatgtg cggctagcgc acggaatcat ccaaaccccg cccaacatca
     2521 atcatcccgc acgcgtggcg gagcgcattg cggtgctcga tctgatcagc aacgggcggt
     2581 gcgaattcgg catgggttcg ggagcgacaa cgacggaact cggggggttc aacgtttcac
     2641 aggaagaaaa gaaggccatg caaatcgagg ggtttcgggt tgcggtgcgg atgctggtag
     2701 aaaatccgtt cacgggattc gaagggcaat acgtgaaggt gccgccgcgc aacgtggtgc
     2761 cgaagccgct gcagaagccg catcccccgc tttggatggc gtgttcccgg cgtgaatcga
     2821 ttctggaagc tgcaaggctg ggactgggcg cgctgacatt ttccttcgcc aacccatcgg
     2881 aagcgcgcca gtgggtaaag gattattacg aaacaattga aaatgaatgc gagccgcttg
     2941 gttacgccgt caatccgaac gtggcaattg cggctccttt cctttgcgac cgggatcaga
     3001 cgcgggcgga agaaatcggg gccgaaaatt acggcttttt cgtctatgga cttggacatt
     3061 acagcttttt cggcgagcac cgccccggaa agaccgacat ttggaagggt tacaagacga
     3121 accctaaaga agccgcgaga ccggaattca ccgccaacca ctgtatcggc accccgaaaa
     3181 aacttcggga aacccttcgc gaattcgagg aagcaggggt cgatcaggtg gtcgccgtgc
     3241 ggcagatcgg gcatctctca ctggacatac agagttcctc gacgcagctg ttctcagaag
     3301 aagtattgcc tgaattcaag gaacgagagc ttgcgaatgc gcgacggcag gccgagcgga
     3361 acgcacgaat ttccgaaaag gcaatggctc gcaaaccgaa ggttgaggca ccgatcgtgg
     3421 agaccgtgat tcagtcagct gggcgccatt aaaaatcgaa ttcctgacgg ctagcggctt
     3481 ggaaagcaca agcgcgcccg gtgctattgg cgtgtttagg cgcaaccttc gagagacgaa
     3541 aagtgagagc gaatcgtccc ggcttgcgga gccaaccgga cgcccttgtg cgcgattgcg
     3601 tcttcctcgt gcgggagaga gaatcaggag aggagaaaag ccgtgacggt aaagacagtt
     3661 gttgacggaa catttcggca aaccaatttg accaatgaat cgcccgaata cttagctaag
     3721 cgcgaagagc ttcgactcgc agaaatcgag cttacgcgcc agcgcgaacg cgtagctgat
     3781 ctgcgccgcc atctgccgaa gggagctgct attcaggact acgagttcga agagggacca
     3841 cgcgatctca acgcaggtga tgaacccgtg cgcaacatcc agctgagcga actcttcacc
     3901 aagcccaatc ggtcgctcgt catctatcac ttgatgtacg gaaaacggca aaccaaagcc
     3961 tgcccaatgt gcactgcgtg gctcgatgcc gcgaatggca tcgcccatca cctcgcgcaa
     4021 aatctcgact tcgccgttgt cgcagcggcg gatatcccta ctctgcgcgc ccacgctcgc
     4081 gcgcgcggct gggacaaact gcgcctcctc agcgccggca ataacacctt caaatatgac
     4141 ttcggcagcg agaaccgcga agggcagcag gactccacga tttccgtttt cacgcgcgac
     4201 gcggacggca cagtgcgcca cttctatagc gctcatccct ggctggcgcc tgacgtactc
     4261 gagcgcggca tcgacctgct caatccgatg tggcacttca tggacctaac accgcaaggc
     4321 cgtggcgatt ggtacgccag cctcaattac ggcacgaagg tccaggccgc gtctacgtaa
     4381 gcgaaatctc gcaaaacatc agcagctgaa gcgccttgtc atggtgggta tctgttcagg
     4441 acagctactt cgaggccagc gtcacgcagg aatcctcata gctgccggaa cccatggcgc
     4501 cttcggggaa gatcccgtgc tcggagatcc aatcgaaaga ctgtttgaac acatccttgg
     4561 tatacggctc gaagaccagg cgctcgccag gcccccagcg gcgcgtatcc atcacatcat
     4621 gaaagcgcgt ggggaattct tttttgtaat agtgcgtata gaggtcgggg cgtagatcga
     4681 tatcgcgctg agcttttcgc agggcgcgga aatatttgcg gacgtcgtcc atatccggat
     4741 cgcgagtgat gagcgacgcc atcatgaagg tagtatcaat gaccttgcga aatccgagct
     4801 gttcgagcag gtagtaaggc ccgctgaaaa gagaagcggc cggaactttg cggtcgatca
     4861 ggagttccat gcggctaaag agcatgccat cggcaaagga aagcttgatg tccgcgggct
     4921 tcatatactg ttcgagagat tggatagcag aatagtgact gcccgactgg tagccgacgg
     4981 aaatgggaac gccagcaagt tcggcggggt catggatagg cgactcgggc ggcacaaaaa
     5041 ttcctgcggg cgcgacggaa taggcgtcgg cgtagagctt gccgtggccg actgaagcag
     5101 agacgtttac ggtccagtga caggcgcagc caatgtcaga accgcgtccc ttttcgaagg
     5161 tttggaaagc gccgactttg tctcccaaat tgtgtttctt ggcttcgtcg gaagcgagcg
     5221 cttcccgaaa ctcgtagtcc aggccttcgg cagcgaagta gcccttttcc tcggctaccc
     5281 actcgtgaag ccgcatatgc ggtgcaattg tgaacttcgc catgtctt
//
LOCUS       DATNDT010000112         9580 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2916408, whole genome shotgun sequence.
ACCESSION   DATNDT010000112 DATNDT010000000
VERSION     DATNDT010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2916408"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1524)
                     /locus_tag="VN788_03525"
     CDS             complement(<1..1524)
                     /locus_tag="VN788_03525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19637.1"
                     /translation="MLLFGGALMVCAAAAQAPSQSAGVKPAKEVPREASRADAKKAKA
                     AYEEGVRAEQRLDWDAAFAAYSDAANSDPTEREYLLRREIAKSRIVQKKVDAAERDAV
                     SGRLDAARSELLAASSIDPSNKMVQKRIVELTAAQPGRVREILEPEIAGEPTLAYRPG
                     KQSFDFRGDTQGAYQQLASQFGVEAAFDSDLHARAVRFRVTDVDFPTAARLLGDMTGT
                     FWRPLTSRLFFVTDNTPQKRKDYDASVVRTILLPSSETPAQMTEMLRMVRDITGITRS
                     DLDAPSRTITLRASPQAIAVASDLISNLEQPTGEVVLEIEVLEVDRSRARQLGITPPQ
                     TAKVYSLSSQQIQEAAQSDQGLIDVINQIFGTSSIPPVIAFGGGKTTFLATLPGAAAN
                     FAEMLSLVRHGRRILLRAQDGQPATFFVGDRIPVSLTTFAPSITGTGVSSSGSAGAVA
                     PLTNYAAGNSPSFVTTADLRANSINDLIVANSADNTVSVLLGNGDGTFGAQATFPTGT
                     "
     gene            1738..3000
                     /locus_tag="VN788_03530"
     CDS             1738..3000
                     /locus_tag="VN788_03530"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing protein"
                     /protein_id="HXU19638.1"
                     /translation="MIPALLVALREGVEAALIVGIVLVYLDRSGRRGLKSYAWGGVIF
                     ACAASIAAAILLQRWAISEDGFEGLLMLLASVLVVTMIIWMNRVARSLRKDIEARVEA
                     YAQRSTSAAGWAIGAFVFFMVVREGAELVLILRAVELSTAGVQVWIGTLLGIAIAVGV
                     GFFFFQGTLRIPLHRFFRATSVILMVVAFQLGLTGVHELSEAQWIWSSKTEMATVGPI
                     VRNEVFFFVVILGVAALVVMREWFGARRPAEDASPNPAERRLLEWEFRRQRRWSFAAA
                     ILCVLVVVSLAAEFAYTHAMNAPQPAKMLTAQNNEVRIPLSELTDSSLHFYTAEVDGT
                     SIRFMVIHRTNGDYATALDACQICGRAGYRQEGQNVICKNCGSAIYIPSIGDRGGCNP
                     IPVKATVAGGEVIVDLSALAGANAMVHS"
     gene            3004..4302
                     /locus_tag="VN788_03535"
     CDS             3004..4302
                     /locus_tag="VN788_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19639.1"
                     /translation="MFARIVRQSFARNPRRKILTAAALVVGMAVATATLTVALEVGDR
                     MAREFRSLGANLLVTPQSDTLPIEIGGIDYRPVDEGAYLQEADLGKLKTIFWRHNILG
                     FTPFLDVPAYFGSREMQAKTSATVIGTWYDHAVPVPDGTTFKTGASVTHPWWKIQGRW
                     FQDGAEEAVVGEALAAKQPDTFAIGKTIYVAEEENSGSARPLVVTGIVSTGDSEDDAV
                     LVPLSIAQSLSGHPGQFRQLFVSALTKPADALASRDPKTLTPTEYDRWFCSPYISSIS
                     YQIAEVLPGTDVHAIRRVADSEGKILSHVSTLLWIVTLAALVGAALAVAATSAAAIID
                     RRFEIGIMKAMGATNTSVSALFLTEHLMLALIGGAIGFIFGSGLARFLGASVFGAPAS
                     PKIILLPIVLGLAALVALAGGLIPLRRAARFDPAPILRGE"
     gene            4299..5453
                     /locus_tag="VN788_03540"
     CDS             4299..5453
                     /locus_tag="VN788_03540"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19640.1"
                     /translation="MMFGRVLWKLLRGNRGRLAVALVAVISGATVIAGLLNLQFDMRE
                     KLTHEFRLLGANLVISRSGTPGTSSGALGSSGSPALMDQGAVLNQIQRLQTPDVVGAA
                     PFLYIVARNQDVPVVVTGTWLDQLPKLDPTWKIDGQWIASRDDDGKCIVGRNAAREFE
                     LSPGDRITLNYLGRRETLAIAGVIDSGTAADNQVFVNLPAAQQLAAVNRQIELVQMSV
                     SGTAASIGSYAAKLASSLPGYDVRPIRQVTEAEGNLLQRTRLLVASMVVLILALTALC
                     VLATMAALAMERREDVGLMKALGGSMARIVALFLAEVGVLGAVGGLIGCIAGIALARW
                     MGERVFGAAIAARWEVFPLTIAMMVIASMAGALPLRALGKVKPAVILRGE"
     gene            5460..6128
                     /locus_tag="VN788_03545"
     CDS             5460..6128
                     /locus_tag="VN788_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005910824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU19641.1"
                     /translation="MLAPLVQIEDLRKEFGSVRALDGVSFDVDAGEWIAIMGPSGSGK
                     TTLINILGGLDTPSSGRALVDGTDVARLDESGLARFRAQKIGFIFQQFHLVPYLTALE
                     NVMLAQYFHSTTDETEARQALERVGLADRVEHLPGQLSGGEQQRVAVARALINHPKLV
                     LADEPTGNLDEANEEIVLRLLRELHSEGHTILMVTHAPSIGALADRRIDLAHGRLAQV
                     VTRA"
     gene            complement(6430..6816)
                     /locus_tag="VN788_03550"
     CDS             complement(6430..6816)
                     /locus_tag="VN788_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXU19642.1"
                     /translation="MATSYLLKTEASAYSFEDLQRDGTTLWDGVSNPVALRNLREMQP
                     GAKLVIYETGDRKSAVGTATVVSVDTSNPKNPEVKIKAGKPLPKPVTLAEIKANKLFS
                     DSPLARQGRLSVVPLSADQYKFLAGS"
     gene            complement(7309..9390)
                     /locus_tag="VN788_03555"
     CDS             complement(7309..9390)
                     /locus_tag="VN788_03555"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020298890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU19643.1"
                     /translation="MNCDLYRRGLPIMAAVWLLTVIGCARNVQVDDAALRNADSDSAN
                     WITYGRTYSEQRFSPLKKINEQSVSRLGLAWSMDFPTLRAYEATPLVKDGVMYTTGAW
                     SVVYAMNAQTGAPLWTYDPHVPKDYSKFACCDVVNRGVALYKGRVYVGALDGRLIALD
                     AKTGAVAWEEQTTPKGAPYSITAAPRIAKGRVLIGNAGSEYAVRGYVSAYDAETGKLA
                     WRTYTVPGDPSQPGESDALRKASSTWQGQWWKTGGGGSPWDSLVYDPDLGMIYVGTGN
                     ASPWYDQVRGKGDDLYIASILALRADTGEQVWHYQTTPGDNWDYDATQPLLLATLTID
                     GKQRRVLMQANKNGFFYVLDRENGSLISASPFTEINWATGIDSNGRPVENAEIRAMKD
                     ATIVKPSTEGAHNWNPISFNPATGLVYLSVLDSASIHATPRDWQMDVHDQTTGADRGY
                     HGPARDQWLKIQSKGRLVAWDPVARREVWHADQPFPRSGGTLTTAGNLVFQGRGDGKF
                     IAYRATDGKPLWEFDTGVGISAGPMTYSVSGTQYVAVMAGWGGPTTLGNRSIGKGKIG
                     NGRLVVFAIGGTATLQQRPKRAVPPVSMPTFKLAVSPREIEHGSNLFAAYCARCHGGD
                     AVSSGLVPDLRYTSESKHEIFQQIVRGGALRELGMPSFSEDLTGAQVRSIEGYILKRA
                     RESAQSDAEKH"
BASE COUNT         1824 a         2716 c         2966 g         2074 t
ORIGIN      
        1 cgtccccgtc ggaaaagtcg cctgcgcgcc aaatgtgccg tcgccatttc caagcaagac
       61 ggacaccgta ttgtcggccg aattcgcgac gattaggtcg ttgatgctat tggcgcgaag
      121 atcggccgtg gtcacgaaag acggtgagtt tcccgccgca tagttggtga gcggagccac
      181 tgctccggca gagccgctgc tgctgacccc ggtccctgta atactcggag caaaagtcgt
      241 aagggagact ggaatgcgat cgcccacaaa aaatgtagcc ggctggccat cctgcgcgcg
      301 gagcaaaatg cgccggccgt gcctgaccag cgagagcatc tcggcaaaat tggccgccgc
      361 gccgggaagc gtggcgagaa acgtcgtctt gcctccgccg aaagcaatca ccggcggaat
      421 cgatgacgtc ccaaaaattt ggttgatcac gtcaatgagg ccctggtcgg attgtgccgc
      481 ctcctgaatt tgctgcgagc tgagggaata aactttcgcc gtttgcggcg gagtaattcc
      541 cagctgccgc gcgcggctgc ggtccacttc gagaacttca atttccaaaa ccacctcgcc
      601 cgtgggctgc tcgagattgc tgatcaggtc cgaggccacc gcaattgcct gcggagacgc
      661 gcgcaaggtg atcgtgcggc tgggcgcgtc gagatcggag cgggtgatgc cggtgatgtc
      721 gcggaccatc cgcaacattt ccgtcatctg ggcgggcgtt tccgaagacg gaagcaaaat
      781 cgtgcggacc accgaagcgt cgtagtcttt tcgtttttgc ggagtgttgt cggtgacgaa
      841 aaagaggcgc gacgtgagcg gccgccaaaa tgttccggtc atatcgccca gcaaacgcgc
      901 ggcagtggga aaatccacgt cggttaccct gaagcgcacc gctcgggcat gaagatcgga
      961 atcgaatgca gcttccacgc cgaattgcga agccaactgc tggtaggctc cctgcgtgtc
     1021 gccgcgaaag tcaaaactct gcttgccggg ccggtatgcg agggtaggct cgccggcgat
     1081 ttccggctct aagatttcgc gaacacgccc cggctgggcg gccgtcaatt ccacgatgcg
     1141 tttctgcacc attttattcg aaggatcaat ggaactggcg gcgagtagct cgctgcgagc
     1201 ggcgtccagt cgccccgaga ctgcatcgcg ttcggccgcg tcgaccttct tttgaacgat
     1261 ccggctcttc gcaatttcgc gccgcagcag gtattcccgc tcggtcggat ccgagttcgc
     1321 tgcgtcggag taggcggcga aagcggcatc ccaatccaat cgctgttcgg cccgcactcc
     1381 ttcttcgtat gccgctttcg cttttttcgc gtcagccctg gatgcttccc gcgggacctc
     1441 tttggcaggc ttaacccccg ccgattgcga aggcgcctga gcagcggcgg cgcagaccat
     1501 caatgccccg ccgaacagca gcaagcagga aacgctccgg attccggcgc gacggtacct
     1561 tctgaccttg ccgggataat tctgatcggc catggttgtg tgagatgcca ttgtacaaag
     1621 gaaggcaagt cgtgcggatg caaagagaga tcggtgtttc tgaaagagct tgaatttagg
     1681 ccaggacccg gatagactga gttttctagt tgcaactcgt ttacaactgg aaattatatg
     1741 attccagctc tcctggtagc cctccgtgaa ggcgttgagg ccgcgcttat cgtcggcatt
     1801 gtactcgtct atttggatcg cagcggccgc cgtggcctga aaagctatgc atggggagga
     1861 gttattttcg cgtgtgccgc cagcattgcc gccgccattc tcctgcagcg ctgggcgatc
     1921 agcgaagacg gtttcgaagg cctgttgatg ttgctggcct ccgtgctcgt tgtcaccatg
     1981 atcatatgga tgaaccgggt ggcgcggagc ctgcggaaag acatcgaagc gcgcgtggag
     2041 gcttatgcgc agcggtccac gagcgccgcc gggtgggcca tcggagcctt cgtgttcttc
     2101 atggttgtgc gcgaaggtgc ggagctcgtg ctcatcctgc gggctgtgga actttccacc
     2161 gcaggcgtgc aggtttggat cgggacactg ctgggaatcg cgattgctgt cggcgtggga
     2221 tttttctttt tccagggaac attgcggatt ccgctgcatc gcttttttag ggccacaagc
     2281 gtgatcctca tggtggtcgc gttccaactg ggtctcacgg gcgtgcacga attgagcgag
     2341 gcgcagtgga tatggtccag caaaacggag atggccactg tggggcctat cgtccgcaat
     2401 gaagttttct ttttcgtggt aattcttgga gtcgccgcgc tggtggtgat gcgggaatgg
     2461 ttcggcgcgc ggcgcccggc cgaggacgct tctccaaatc ccgccgagcg ccgcctgctc
     2521 gaatgggaat ttcgccgcca gcgtcgctgg agctttgccg ccgcaatcct gtgcgtgctg
     2581 gtggtcgtgt cgctggcggc agaatttgcg tatacgcacg ccatgaatgc cccgcaaccc
     2641 gcgaagatgc tgacggcaca aaacaacgaa gtacgcattc cactctcgga attgaccgat
     2701 tccagcctgc acttttatac ggccgaggtg gacggcacct cgattcgatt catggtaatt
     2761 caccgtacca acggcgatta cgccacggct ctggatgctt gccagatttg cggccgcgcc
     2821 ggctaccgcc aggaagggca aaacgtcatc tgcaaaaact gcggttccgc gatttacatt
     2881 ccttcgatcg gcgatcgcgg cggctgcaat ccgattccgg tgaaggcaac tgtcgcggga
     2941 ggagaagtaa tcgtcgatct ttccgcgctc gccggcgcca atgcgatggt tcactcttaa
     3001 aaaatgttcg caagaatcgt acgccaatcg ttcgctcgca acccgcgccg caagattctg
     3061 accgcagcgg cgctggtggt cggaatggcc gttgcgacgg cgaccctgac tgtggctttg
     3121 gaagtgggcg atcgcatggc gcgcgaattt cgcagcctgg gcgccaatct gctggtcact
     3181 ccacaatcgg atacgctgcc catcgaaatc ggcggcatcg attaccgccc ggtggacgaa
     3241 ggcgcatatc tgcaagaggc ggacctgggg aagttgaaaa ctattttctg gcgacacaat
     3301 attctcggct tcacgccgtt tctcgatgtt cccgcttatt tcggctcgag agaaatgcag
     3361 gccaaaacca gcgccacagt cattggcacg tggtatgacc acgcagttcc agtgccggat
     3421 ggaactacat tcaagaccgg tgcgagcgtc acgcatccgt ggtggaaaat tcagggccgg
     3481 tggttccagg atggcgcaga ggaagcagtc gtgggcgagg cactggctgc gaagcagcct
     3541 gatactttcg cgatcggcaa gacgatctat gtggcggagg aagaaaattc tggctctgcg
     3601 cgcccgctgg ttgtgaccgg aatcgtttcc actggtgaca gcgaagacga cgctgtgctg
     3661 gttccccttt cgatcgcgca aagtctctcc ggtcatcccg ggcaattccg ccagcttttt
     3721 gtgagtgcgc tcaccaagcc ggctgacgcg ctggccagcc gcgatccgaa gacgctgacg
     3781 ccgacggaat acgaccgctg gttctgctcg ccttacattt cgtcgatcag ttaccagatt
     3841 gcagaagtgc ttcccggaac ggacgtgcat gcgatccggc gcgtagcaga ttccgagggc
     3901 aaaattctct ctcatgtgag cacgctgctc tggatcgtca cgctggccgc cctggtgggc
     3961 gccgccctcg ccgtggcggc cacttccgca gcggctatta tcgacaggcg tttcgagatc
     4021 ggcattatga aagcaatggg cgcgacgaac acttctgtct cggcactgtt tttgacggag
     4081 cacttgatgc tcgcgctcat cgggggagcg attggattta tttttggctc cggccttgcc
     4141 cgttttctcg gagcgagcgt tttcggcgcg ccagcatctc caaaaatcat tcttttgcca
     4201 atcgttctcg ggcttgcagc cctggtggcg ttggccggcg ggctgattcc cttgcggcgg
     4261 gcggcgcgtt tcgatcccgc gcccatcctg cgaggcgaat gatgtttggc cgtgtactct
     4321 ggaaactgct ccgaggcaat cgcggccgcc ttgccgtcgc cctcgtggcc gtgatcagtg
     4381 gcgcgaccgt aatcgcggga ctcctgaatc tccagttcga catgcgtgaa aagctgacgc
     4441 acgagtttcg cctcctcggc gcaaacttgg tcatttcgcg cagcgggacg ccgggaacga
     4501 gcagtggcgc cctgggctcg tcgggttcgc cagcgttgat ggatcagggc gcagtattga
     4561 atcagatcca gcggctgcag acgcccgatg tggtgggagc ggcaccgttt ctgtatattg
     4621 tcgcgcgcaa tcaagatgtt cccgtcgtgg tgaccggcac ttggctcgac cagcttccga
     4681 aactcgatcc tacttggaag attgatggcc aatggattgc gtcgcgcgat gatgatggga
     4741 agtgcatcgt cgggcgcaat gccgcccggg agtttgaact ttcgccgggc gacaggatta
     4801 cgttgaacta tcttggccgt agggaaacgc ttgcaatcgc gggtgtgatt gattccggca
     4861 cggcagcgga caatcaagtt ttcgtgaacc ttccggcggc ccagcagctt gccgccgtga
     4921 acaggcaaat cgagctggtg cagatgagcg tgagtggaac ggcggcgagc attggcagct
     4981 atgcggcgaa attggcgagt tcccttcccg gctatgatgt gcgcccaatc cggcaggtga
     5041 cggaagccga gggcaatctg ctgcagcgca cgaggttgct ggtcgcgtcg atggtggtgc
     5101 tgattctggc gctgacggca ctgtgcgtgc tggcgacgat ggccgcgctc gcgatggagc
     5161 ggcgcgaaga cgtaggtctt atgaaggctc tcggtggatc gatggcgcgc atcgtggcgt
     5221 tgtttctcgc cgaggtggga gtgctcggcg cggtgggcgg actgattggc tgcatcgccg
     5281 gaatcgcgct ggcgcgttgg atgggcgagc gcgtattcgg cgcggccatt gcggcgcgct
     5341 gggaggtttt tccgctgacg attgcgatga tggtgattgc gtcgatggcc ggcgcactgc
     5401 cgctgcgcgc gctgggtaaa gtgaaacccg cggtaattct gcggggcgaa taagggacaa
     5461 tcctggcacc gctggtacaa atcgaggatc tgcgcaaaga gttcgggagt gtgcgcgctc
     5521 tcgatggggt gtcgtttgat gtggacgccg gggaatggat cgcgattatg gggccttcgg
     5581 gctccggcaa gacgacgctc atcaatattt tgggcgggct ggacacgccg agctcgggac
     5641 gcgcgctggt ggacggcacg gatgtggcgc ggctcgacga aagcgggctc gcccgatttc
     5701 gggcgcagaa aatcgggttt atttttcagc agtttcacct ggtcccctac ctgacggcgc
     5761 tggaaaacgt gatgctggcg caatattttc acagcacaac ggatgagacc gaggcgcggc
     5821 aggcgcttga gcgcgtcggc ctcgcggatc gcgtcgagca tttgccgggc cagctttccg
     5881 gtggcgagca gcagcgcgtg gcggtggcgc gcgcgctgat caatcatccg aagctggtcc
     5941 tcgccgatga gccgacgggc aaccttgatg aagccaacga ggagattgtt ctgaggctgc
     6001 tgcgcgaact tcactccgag ggtcacacga ttctgatggt gactcacgca ccgtcgattg
     6061 gcgcgctggc cgaccggcgc attgatttgg ctcatggccg attggcgcag gttgtgacgc
     6121 gggcttagcg agccgcacct gaccctctgg aaaaagtgtc gagtagattg tgcgccgatc
     6181 gtttgttttg aatgagttgc agcacattct actcggctca agtgtcgatt agagcgcggc
     6241 ggattcgagc agtttttggg gcgcctggcg cgcggtgaag gcggacggac tgcgggttcc
     6301 ctcggccgca gcgccctatt ttcggcgtca gaagacatag ttttcatgat cacgcagcca
     6361 aagtagcaag ctgcagagga atgcaaggaa caaatccctg tggactttct ctggcgctag
     6421 ttcaagtggt caggaaccgg cgaggaattt gtattggtcg gcgctgagcg ggacgacgga
     6481 gaggcgccct tgacgggcta gaggcgagtc ggagaatagc ttgttagctt tgatttccgc
     6541 taaggtgacc ggctttggta gcggcttacc tgctttgatt ttcacttcgg gatttttggg
     6601 attcgaagtg tcgacggaga cgactgtcgc tgtgcctacc gcgcttttgc gatcgcccgt
     6661 ttcgtagatc actagcttcg cgccgggctg catttcgcgc agatttcgta gcgcgactgg
     6721 attggagacg ccgtcccaga gcgtggtgcc gtcacgctga agatcctcga aggaatatgc
     6781 ggaagcttcg gttttcagca ggtagctagt agccatggaa ggtcaccttt ctcttaccac
     6841 tgtagacgcg taaagggtgt ggcaaatcgc cattctacac ttccggggcg tggttgcagc
     6901 acgcccgggc aagcccgagc accgatctcc caggctcgac taaccgtcga cctgggagcc
     6961 ctgactgggc gggagccctt cgaagctccc ttcctgcgtc aggacaagca gggcaggcaa
     7021 gctcctgcac tccacattcc tctgcctgcc gccgctcggc gttggtcgat cgaagagaga
     7081 ttcctcgact gcgtgtcccg gcgcttcgcg caaaaacaaa ggcgcgggac actccgctcg
     7141 gaatgactgc gcaaaccggc tttcacccga tcgtgcttta cgacagggtg acggcgcgtg
     7201 acggaactgc gaaagcgcaa aaactgacat tggctacgct cctatcctta gtcagggcgg
     7261 ccagaatgac gaaagcagcg gccagtgata aatccaggta ctcagtactc agtgcttttc
     7321 ggcgtctgac tgcgctgatt cacgggctcg ttttaggatg taaccttcga tggaacggac
     7381 ttgtgcgccg gtaaggtctt cggagaagga tggcattcct agctcgcgca aagcgccgcc
     7441 acgaacaatc tgctgaaaga tttcgtgctt ggactcgctg gtgtaacgca gatccggcac
     7501 caatccgctg ctcacggcgt cgccgccgtg gcagcgcgcg cagtatgcgg caaaaagatt
     7561 cgatccgtgt tcgatctcgc gcggagaaac agcgagtttg aaggttggca tcgacacagg
     7621 cggaacggct cgtttagggc gctgttggag cgtcgcggtg cccccgatcg caaaaacgac
     7681 gagtcttccg ttcccgattt ttccctttcc gatggatcga ttgccgagcg tggtgggtcc
     7741 gccccatccg gccattaccg cgacgtattg agttccgctc accgagtagg tcatcggtcc
     7801 cgctgaaatc ccgacgccgg tgtcaaactc ccacagcggc ttgccgtcgg tggcgcgata
     7861 ggcgatgaat tttccgtcgc cccggccctg aaaaacgaga ttacccgccg tggtcagcgt
     7921 tccgccactt ctcggaaaag gctggtcagc atgccacact tcgcgacgag caactggatc
     7981 ccatgcgacc aagcgtccct tcgactgaat cttgagccac tgatctcgcg ctggcccgtg
     8041 atagccgcgg tccgcgccag tggtctgatc gtgaacatcc atttgccaat cgcggggcgt
     8101 cgcgtgaatc gaggcgctat ccaacacgct gaggtacacc agtcctgttg caggattgaa
     8161 cgaaatagga ttccaattgt gtgcgccctc ggtggaaggt ttcacaatgg tggcgtcctt
     8221 catggcccgg atctccgcgt tctccaccgg gcggccgttc gaatcgattc cagtcgccca
     8281 attgatttca gtgaatggac tggcggagat gagcgagcca ttttcgcggt ctaagacgta
     8341 aaaaaaaccg ttcttgttgg cctgcatcaa aacgcggcgc tgctttccat caatagtaag
     8401 tgtggcgagc aacagcggct gagtggcgtc gtaatcccaa ttgtcgcccg gtgtagtttg
     8461 ataatgccaa acctgttcac cggtgtcagc acgcaatgcg aggatggagg cgatgtaaag
     8521 gtcgtctccc tttccgcgaa cctggtcgta ccaaggagat gcgttgccgg tccctacata
     8581 aatcatgccg agatcgggat catagacgag gctatcccat gggcttccgc caccgccggt
     8641 tttccaccat tggccctgcc atgtgctcga tgccttgcgc aatgcgtcgg attcaccggg
     8701 ctgagaagga tcgcccggta cggtgtatgt tctccacgcc agttttccgg tttccgcatc
     8761 gtaagcagaa acatagccgc gcaccgcata ttccgaaccc gcgttcccga tcagcacccg
     8821 gcctttggct atgcgcggtg cggcggtgat ggaatacgga gcgcccttgg gtgtggtttg
     8881 ctcctcccac gcaaccgcgc cggttttggc gtcgagcgcg atcagccggc cgtccagcgc
     8941 gcccacatag acgcgtcctt tatagagcgc gactccgcga ttcacgacat cgcagcatgc
     9001 gaatttcgaa tagtcctttg gaacgtgcgg atcgtacgtc catagcggcg cgccggtttg
     9061 tgcgttcatg gcatagacga cgctccaagc gcccgtggta tacatcacgc catcttttac
     9121 cagcggagtc gcttcgtatg cgcgcagggt tggaaaatcc attgaccacg ctagccccaa
     9181 gcggctaacg ctctgctcat tgattttctt gagagggctg aagcgctgct cggagtacgt
     9241 acgaccatag gtgatccagt tcgcggaatc agaatccgca tttcgaagcg cagcgtcgtc
     9301 cacttgaacg tttcgagcgc aaccaatcac ggttagaagc cagactgccg ccattatcgg
     9361 taatccgcgc cgatacaagt cgcaattcat aaggtctccg ctgcaacttt tcttgaacgg
     9421 tccgctactc cgctcgcgaa ccgattttga ccttgcggcg tacacttata caccgccccc
     9481 ttctggcagg cctaagtgtt ccttgtcgta tggttcttcg atctcgggac cacgaaaaga
     9541 gatctcaccg gcatccaacc acggacttag ggcggatggc
//
LOCUS       DATNDT010000113         2578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_329492, whole genome shotgun sequence.
ACCESSION   DATNDT010000113 DATNDT010000000
VERSION     DATNDT010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2578
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_329492"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VN788_03560"
     CDS             <1..849
                     /locus_tag="VN788_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase family peroxidase"
                     /protein_id="HXU19644.1"
                     /translation="KGILLSGTFTPSPSGRSLTRAPHIQRQSTPVTVRFSDFAGVPTV
                     ADNDPNAASPRGIAIRFHLAEHVHTDIIGHSVDGFPVRTAEEFVEFLRALGASGPDAP
                     KPSPIESFLGAHPAALAFVQTPKPIPTSFAKESFFAVNAYKFTSQDGVSRYGRYRILP
                     GTGNEYLTADDAAAKSANFLFDEIDERIAKGPVKLHIAVQLAEKGDVVDDSTIHWPAD
                     RRQVEIGVIELTGIAPNNDAEQRHIIFDPIPRVEGIESSGDPLLEPRAAIYLASGRRR
                     RAAAVE"
     gene            complement(859..1939)
                     /locus_tag="VN788_03565"
                     /pseudo
     CDS             complement(859..1939)
                     /locus_tag="VN788_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            2168..>2578
                     /locus_tag="VN788_03570"
     CDS             2168..>2578
                     /locus_tag="VN788_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase beta-grasp
                     domain-containing protein"
                     /protein_id="HXU19645.1"
                     /translation="MSPKEVLEFTKKNEVLQLDLRFTDIPGLQHHISYPISQLEADSF
                     EEGFGIDGSSIRGWAAINESDMLLVPDPATAFVDPFSQVKTLVMYGDVIDPITRQHYL
                     RDPRWIARRAEMYLKNTALGDTAYFGAEAEFFIFD"
BASE COUNT          548 a          796 c          708 g          526 t
ORIGIN      
        1 aagggcatcc tgctctccgg tacatttact ccatcgccca gcggacgttc gctgacgcgc
       61 gctcctcaca tacaacggca atccacgccg gtaaccgttc gcttctcgga tttcgcgggc
      121 gttcccaccg tcgccgacaa cgatccgaac gcagccagtc ctcgcggaat cgcaatacgg
      181 tttcatctcg ccgaacatgt tcacacggac atcatcggcc actccgtcga cggattcccg
      241 gtgcgtacgg cggaagaatt tgtcgagttc ctccgagcct tgggcgcaag cggtccggat
      301 gcgccgaaac cgagcccgat cgaatccttc ctcggcgcgc atccggccgc gctggcgttc
      361 gtccagactc ccaagccgat tcccactagc tttgcgaagg aatccttctt cgccgtgaac
      421 gcgtataaat tcaccagcca ggatggcgtc agccgttacg gccgctaccg cattcttccc
      481 ggcaccggca atgaatacct gactgcggac gacgctgccg cgaagtccgc caatttcctc
      541 ttcgacgaaa tcgatgaaag aatcgcgaag ggcccggtga aactccacat cgcggttcaa
      601 ttggccgaaa agggcgacgt tgtggacgat tcgaccatcc attggcctgc ggaccgccgt
      661 caagtcgaga tcggtgtcat cgagttgacc ggcatcgctc cgaacaacga tgccgagcag
      721 cgccacatta ttttcgatcc gattccccga gttgaaggca tcgaatcgtc gggtgacccg
      781 ctcctggagc cgcgtgccgc catctatctc gcgagtggac gccgccgccg cgcagccgcc
      841 gtcgaataaa gaacgcggct atggcttcgc ttcaaagacc aaattaaagg gcgtttgagt
      901 cgcgcgccgg aagtggacga agccccctgc cacgaccaca tcccgaatgc gattctcgcc
      961 ggcttgtgcg cccagagcgg cgcctacttc ctgcgaaatc gacgcgggaa cgcaaagtaa
     1021 cgtggaggct gagtagtaca cccggccgat tgggttgtgg ttggcttccg cggcatcctc
     1081 ggcgaacggt tccacgatca tccatgttcc atcgcgtttc aagctggagt gaacatggcg
     1141 ggcggcgccc tttgggtcgc ccatatcgtg caagcaatcg aagaaggcca cgaaatcgta
     1201 atccgtgccg ggaaacgctt tcgcgggcgc aacttcaaaa cggatccggc ccgctacccc
     1261 ttcccggtcc gccgcctctc gcgccagatc tatggaagcg gcgtgatagt cgaatccaaa
     1321 agaattcgga ttgcggatac gccttcgcca tcagaatcgt cgatgctccg aggccgcacc
     1381 caatatcggc gactttcgct ccgcggcgta gtttctcttc gactccatcg agcgccggaa
     1441 tccagctcga cgtcagattc gcgatgtaat taggacggaa gaagcgttcg gttccatgga
     1501 acagatcgtg gtgatggtcg tgccacccga cgccattgcc ggtgcggaat gcatcctcga
     1561 ttttgtccgc gtcgtggaag cacgatgaaa tgatttggaa agcacccggt aaaaaaaccg
     1621 gactgttttc aaccgccagc gcgaatgcct gttcgggagg cagtgaatag gtctccgcgg
     1681 cggaatcgta gactacatag ccgctagcgg cattggcgtt aagccactcc cgcacatagc
     1741 gctcacttgt accggtacgg ccggcaagct cggcgggcgt gaccggcttc gagtcggcca
     1801 tcgccttgta gagcccaagt ttgtcgccga gacgaacgag aacggcgtgc atcgccgctc
     1861 ccatatccat cacggcctta cccatgaatt cgttaagctt cgattcatca attggagcgg
     1921 cggtccccgt cgatgccatt taataatctc cctggaggcc gattatagac aatttgacga
     1981 gcggcgggaa gggtgaaaat tcaaagggcg ttgcgtcccc gccgccccaa cattccctca
     2041 taagaaatgt tttgcaggcc cacaaaatct tttgctttga cgcggcatcc tcgcgggaaa
     2101 tacgctcaaa gactgagcca tccttagcgg cggccggttg cacgcttaca tcaactccgg
     2161 agaatcaatg agccccaaag aagtgttgga atttaccaag aagaacgagg tccttcagct
     2221 tgacctccgt tttaccgaca tacccggtct gcagcatcac atctcgtatc cgatctcgca
     2281 acttgaggcc gattctttcg aagagggttt cggcatcgat ggctcgagca tccgcggctg
     2341 ggcggctatc aatgagagcg acatgctgct cgtccccgat cccgcgaccg cgttcgtcga
     2401 tccgttcagc caagtcaaga cgctcgtgat gtacggagac gtgatcgatc cgattacgcg
     2461 tcagcattat ctgcgcgacc cgcgctggat cgcgcgcaga gccgagatgt atctgaagaa
     2521 caccgctctc ggcgacaccg cctatttcgg agcggaggcg gaattcttca ttttcgat
//
LOCUS       DATNDT010000114         6024 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2062461, whole genome shotgun sequence.
ACCESSION   DATNDT010000114 DATNDT010000000
VERSION     DATNDT010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6024)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6024)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6024
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2062461"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VN788_03575"
     CDS             complement(<1..197)
                     /locus_tag="VN788_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19646.1"
                     /translation="MALFLILAMIVGSQVVLRNLVTLRRRQREADTIWRGRQYERAIR
                     LYYHKTGHYPQSLDELQKGLP"
     gene            complement(306..809)
                     /locus_tag="VN788_03580"
     CDS             complement(306..809)
                     /locus_tag="VN788_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19647.1"
                     /translation="MVLVAYSGYYYFSSRSHVAGFPGVFAANSKFQPLDVQEPDLHMN
                     LLERIRKTEYAGSHRNIFSATAPPIEKPKTEASKFVGPRSAPPPPPVQIPAEFFGYAW
                     RPHSEKRVAFFTSGDDVLIVHEGDSFLGRFRLDRVGNDSADVEELSTGRHATVPLVQP
                     PDQGSNQ"
     gene            complement(854..1405)
                     /locus_tag="VN788_03585"
     CDS             complement(854..1405)
                     /locus_tag="VN788_03585"
                     /inference="COORDINATES: protein motif:HMM:NF022201.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspMb/PilO family protein"
                     /protein_id="HXU19648.1"
                     /translation="MTTGHNSPSWKKWIGIALAVPLVLDLGLAVYLWQGGRGNPREIR
                     AEAGRLATQAKLLRADVDRGEKIRASLPQVGKDADSFYKDSFLDTSSGYSRIESDLGA
                     IAAQAGVRTSGFAFKQAKTKQPGVTQISVTTSLQADYPAIVKFINGIERSKNFYLLDQ
                     MQINSVSTGGLNLQLELHAFFRT"
     gene            complement(1402..1968)
                     /locus_tag="VN788_03590"
     CDS             complement(1402..1968)
                     /locus_tag="VN788_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19649.1"
                     /translation="MRLHLNLATAPQPNNRPFMAGAVVTGAIGLIALAFLSHAAYASW
                     RNTRALRAQVTNLEATIQANRRRQQSLGDYFRSPGARQILNRAAFLNSLIDERSFPWT
                     KIFTDLENTLPPGVRVISISPQLTNGRAEVTLKVGALTDESKIRFLQAIEKSNVFSGM
                     VVKDERRSTGAPTEDPIVLNLTVWYSTT"
     gene            complement(2060..3049)
                     /locus_tag="VN788_03595"
     CDS             complement(2060..3049)
                     /locus_tag="VN788_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19650.1"
                     /translation="MTNPAGTEGLESRFYQIGLVRRIDAWLHKMPHPGIAVEISGQHV
                     AAARWGKIRGHLDEVAVQLLPAGAVMPSPVETNVTQPDGVRSALRRVFNSVPASGAHV
                     ALLVPDPCVRVFILPFDNLPRSANEALPLLRWRLKKSVPFDVEETVVSWMRQEGRHDS
                     LEVVASVARQGIIREYEELVESLGAHASVVLSSTLATLPLLEEEGATLLVRLCGKMLT
                     TVIVRGSGLCVYRSSEMRAEAASLEPQAMLDEVFPAVAYYQDSWNASVDRARLAGFGA
                     REELFAQALAAEVGVNVGPMSASQGASRLDRAAKDLLPNGLDALAGWAMNGAA"
     gene            complement(3042..4676)
                     /locus_tag="VN788_03600"
     CDS             complement(3042..4676)
                     /locus_tag="VN788_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXU19651.1"
                     /translation="MATAGDNPIPIGVLDDRAEREAAKRLAERYRCSFIDLREQRIDP
                     ELFRSIPAELMFRYNFVPLESHDSTLVVAIADPSQLHLTDELSLLLGKRLHLKVATPS
                     QIGDLLKRTEQSQRVLDQATEGFTLQVVGEEEDSDENISMEKLTRGDTGVSPVVRLVD
                     TVIFTALERRASDVHIETRNAEVVVKYRIDGVLQYAMQPLAKDWHGTILSRIKVMSEL
                     DIAERRTPQDGRFRVKYKGRFVDFRVSIMPSVHGEDAVLRVLDKETLSEKFRTLTLDV
                     VGFDHDEVTRFRRYIHEPYGMVLVTGPTGSGKTTTLYAAINEIKNDEDKIITIEDPVE
                     YQVRGITQVPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAINSALTGHLV
                     FTTVHANNVTDVIGRFINMGVEPYNFVSALNCIMAQRLVRLVCENCKRRVTYSPDVLR
                     ESGLDPSEWGEAAFAEGAGCLECSGSGYRGRTAITELLDLSDRIREMIIDRRPTSEIK
                     RAAREAGMMFLRESGLRKVRAGVTTLREINKVTFLD"
     gene            complement(4683..5897)
                     /locus_tag="VN788_03605"
     CDS             complement(4683..5897)
                     /locus_tag="VN788_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXU19652.1"
                     /translation="MAEFVCKIGDTSGRIFQQVETAQSEGEARQKLAERGFYVFSVRS
                     HFDLLAQIGSGRPERKIRPDHFLIFNQQFHTLIKAGLPILRALDLLAERAAAPQIRPI
                     LIEVRQRVKDGALLSEALAAQGSFPPVYVTVITAGERSGNHIGVLEQYIEFLRVSTGF
                     RSRLMTALIYPSVLVAVVLLVITYIVTYAMPQFANLYHELDVPLPTPTRLLLAAALPL
                     RTYFLVLIAILAVVALSIFVWTRSKRGALAIDQMKPRIPYLGDIWLKTQIAQFVRTLS
                     TLLGGGTPLVPALLTSSSAMGSALISKSVDLAAGQVKEGKALHASLAETRLIPPLALE
                     MIEVGEASGALTAMLTSVAEFYEEEVGIRLQRTLTWIPIVILIVMALVIGFILIALYL
                     PMFSLQLGNAGG"
BASE COUNT         1352 a         1628 c         1840 g         1204 t
ORIGIN      
        1 tcgggcaatc ccttttggag ttcgtcgagg gactggggat agtgcccggt cttgtgatag
       61 tagaggcgga tggcgcgctc gtattgccgg ccgcgccaaa tggtgtccgc ctcacgctgg
      121 cgcctgcgga gggtaacgag atttcgcaag acgacttggg agccgacaat catggcgaga
      181 atcaaaaaca gcgccatgat gaaggcatag ccggattccg caggacggcg cctggttggt
      241 tgctcccgct tgaagaagcg caccgagcgg cgcgcagaaa atcgaattgg gaagcaagac
      301 aacatctact gattcgaacc ttggtccgga ggctgaacga ggggaacggt ggcgtgcctg
      361 ccggtggaaa gctcctcgac atcagcggaa tcgttgccga cacgatccag acggaatcgg
      421 ccgagaaagg aatcgccttc gtggacgatc aggacgtcgt ctccgctggt gaaaaacgca
      481 acgcgttttt cggaatgggg acgccaggcg tagccgaaga attcggcggg aatctggaca
      541 gggggcggcg ggggagcgga ccgcggccca acgaatttcg aagcctccgt ttttggcttt
      601 tcgatgggag gcgcggtggc agaaaaaatg ttgcggtgcg aaccggcata ctcggttttg
      661 cggattctct ccaaaagatt catatggaga tcgggttcct ggacgtcgag aggctggaat
      721 ttggaattgg cagcgaagac gccgggaaat cccgcgacgt ggctccgact cgagaaatag
      781 tagtaacccg agtaggcaac cagaaccatg agggccgcga aaatataaag ttcagtgcgc
      841 cgtgacatac gaatcaagtc ctaaaaaacg cgtgcagttc gagctgcaag ttcaggccgc
      901 cggtggatac ggaattgatc tgcatctgat ccaacagata aaagttcttg gagcgttcga
      961 tgccattgat gaacttgact atcgccggat aatcggcctg aaggctggtg gtaacagaaa
     1021 tttgagtgac gcccggttgc ttggtcttcg cctgtttgaa ggcaaagccg gatgttcgga
     1081 cgccggcctg ggcggcaatg gctcccaaat ccgactcaat gcgggaatag ccggaggagg
     1141 tatcgagaaa ggagtcttta taaaaggaat ccgcatcctt gccgacctgg gggagcgaag
     1201 cccgaatttt ctcaccacgg tcaacatctg cccgtagcaa tttggcctgg gtcgccagac
     1261 ggccggcctc agcgcgaatt tcccgcggat ttccgcgacc gccctgccaa agatatacgg
     1321 ccaagccgag atcgaggaca aggggcaccg ccagggcgat ccctatccac tttttccaac
     1381 tcggcgagtt gtggcctgtg gtcatgtagt tgaataccag acggtaagat tcaggacgat
     1441 aggatcttcc gtcggggccc cggtcgaacg ccgctcgtcc ttcaccacca tgcccgaaaa
     1501 tacgttggat ttttcaatgg cctgaagaaa ccgtattttg ctttcgtcgg tgagcgcgcc
     1561 gaccttgaga gtgacctccg cgcggccatt cgtcagttgg ggcgaaatgc tgatcactcg
     1621 aacgccggga ggcagcgtgt tttccaaatc ggtgaagatt ttcgtccatg gaaaactgcg
     1681 ctcgtcgatc aaagagttga ggaacgccgc tcggttgaga atttgccgcg cgcccgggct
     1741 gcggaaataa tctcccaagg actgctggcg ccggcggttg gcctggatgg tggcttccaa
     1801 gttggtgacc tgtgcgcgaa gagctcgagt gttgcgccac gatgcgtaag cggcgtgaga
     1861 gagaaacgca agggcaatca atccgatggc gcccgtgacc accgcgccag ccataaaagg
     1921 ccgattattc ggctggggcg cggttgcgag gtttagatga agtctcatga gtgatattga
     1981 aaattgctat cgtttctcct gccgattcct gaagagccgc aatgcttgat tgcatagacg
     2041 gccaggcagg ccaggcgcat caggccgccc cattcatcgc ccaacccgcc agagcgtcca
     2101 agccgttggg caggaggtcc ttggcggcgc gatcgagccg cgaagcgcct tgggaggccg
     2161 acatcgggcc aacattgaca cccacttcgg cggccagcgc ctgggcaaaa agttcttctc
     2221 tggcaccgaa tcctgccagg cgggcgcgat cgacggaagc gttccaagaa tcctgataat
     2281 aagcgactgc gggaaaaacc tcgtcgagca tggcctgcgg ctcgagagag gcagcctcgg
     2341 cgcgcatttc gctggagcgg taaacgcaaa gaccgctgcc ccggacaatc acggtggtaa
     2401 gcattttgcc gcagaggcgg acgagcaagg tggcgccctc ttcttcgaga agaggcaagg
     2461 tggccaacgt ggaactgagg acaacgctcg cgtgggcacc gagagattcc acaagttctt
     2521 cgtactcgcg gatgatgccc tggcgggcga cggaggcgac aacttcgaga ctatcgtggc
     2581 gcccttcctg acgcatccaa gagacgaccg tttcctcgac gtcgaatggg acgctttttt
     2641 tcagcctcca gcgcaacaac ggcaaggctt cgttcgcact gcgggggaga ttgtcgaaag
     2701 gcaaaatgaa gacgcgcacg cagggatcgg gaaccaagag agccacgtgc gctccagagg
     2761 cgggaacgct attgaaaacc cggcgcaaag cggaacgcac gccgtcaggc tgggtcacat
     2821 tggtctccac gggcgaaggc atgacggctc cggcggggag caactgaaca gctacttcgt
     2881 ccaaatgccc gcgaattttt ccccatcgcg ccgcggccac atgctggccg gaaatttcca
     2941 cggcgattcc cgggtgcggc atcttatgga gccaggcgtc aatacggcgt accaagccta
     3001 tctggtagaa gcgcgattcc aaaccttccg tgccagcagg gttagtcaag aaaggtcacc
     3061 ttattaattt cgcgcaaagt ggtcacgcca gcgcgcactt tgcgaaggcc ggactcacga
     3121 agaaacatca tgccagcctc gcgagcggca cgcttgattt ccgaagttgg gcggcggtcg
     3181 atgatcattt cgcgaattcg atcggaaaga tcgaggagct cggtaatcgc cgtgcgcccg
     3241 cgatatccgc tgccggaaca ttccaagcag cccgcgccct ccgcaaatgc ggcttccccc
     3301 cactcagagg gatcgaggcc ggattcccga aggacatccg gcgaatacgt gacgcgcctc
     3361 ttgcaatttt cgcacaccaa acgcaccagc cgctgcgcca tgatgcagtt gagcgccgag
     3421 acaaaattat acggctcaac tcccatgtta atgaatcggc cgatgacgtc tgtgacgtta
     3481 ttggcatgta cggtggtgaa gacgaggtgt ccggtaagcg ccgaattgat ggcaatttgg
     3541 gcagtttcct gatcgcgaat ttcgccgacc atgattttgt ccggatcatg gcgaagaatc
     3601 gagcgaaggc cgcgggcaaa tgtgaggccc tttttctcgt tgaccggaac ttgagtgatc
     3661 ccacgcactt gatattccac gggatcttcg atggtgatga ttttgtcttc gtcgtttttg
     3721 atttcgttga tcgccgcgta gagagtagtc gttttccccg aaccggtggg tccggtgacg
     3781 agcaccatac cgtacggctc atgaatatag cggcggaagc gggtgacttc gtcatgatcg
     3841 aagcccacca catcgagagt gagggtgcgg aatttttcgc taagggtttc cttgtccagg
     3901 acgcgcagca cggcgtcctc accatggacg gagggcatga tggagacgcg gaaatcgacg
     3961 aagcgtcctt tgtacttgac ccgaaaccgg ccgtcttggg gggtgcggcg ctcggcaatg
     4021 tccagctcgc tcattacctt gatgcgggac aaaattgtgc cgtgccagtc tttcgcaaga
     4081 ggttgcatgg cgtattgcag cacaccgtca atccggtatt tcaccaccac ctcagcgttt
     4141 cgcgtttcaa tgtggacgtc gctcgccctg cgctcaagag cggtgaaaat gacagtatcg
     4201 acgaggcgga cgacggggct cacgccggta tcgccgcgcg tgagcttctc catggagatg
     4261 ttttcgtcgc tatcttcttc ttcgccgact acctggagcg tgaaaccttc ggtggcttgg
     4321 tcgagcacac gctgagactg ttccgtccgt ttcaaaagat cgccgatttg cgatggcgtc
     4381 gcgaccttta ggtgcaggcg ctttcccaga agcaacgata attcgtcggt aagatgcaat
     4441 tggctgggat ccgcgatggc gacgaccaga gtggaatcgt gggattccag cggcacgaag
     4501 ttatagcgaa acatgagttc cgccgggatg ctgcggaaaa gctctgggtc gatgcgctgc
     4561 tcacgaaggt cgatgaagga gcaacgatat cgctcggcga gacgcttggc ggcctcgcgc
     4621 tcagcccggt cgtccaggac gccaatgggt atcggattat cgcccgctgt tgccataaat
     4681 cactagcccc ctgcgttgcc tagctgcaga gaaaacatgg gcagatagag agcgattaag
     4741 atgaagccta tcacaagggc catgacgatt agaattacga tcgggatcca ggttagcgta
     4801 cgttgcaacc tgatgccgac ctcttcctcg taaaattccg caacgctggt cagcatcgcg
     4861 gtgagggcgc cggatgcctc tccaacctcg atcatctcca gcgcaagagg cggaatcaag
     4921 cgggtttccg ccagactggc gtggagcgcc ttgccctcct tcacttggcc cgcggccaaa
     4981 tccaccgatt tggatatcaa cgcgctgccc atggccgagg aggaggtgag cagggcaggc
     5041 accagtggag ttccgccacc gaggagggtg gacagcgttc gcacaaactg cgcgatctgg
     5101 gtcttcagcc agatgtcgcc gagataagga atccgcggct tcatttggtc tattgcgagg
     5161 gcgccccttt tggatcgggt ccagacaaaa atcgaaaggg caaccaccgc cagaatcgcg
     5221 atgaggacca ggaaatacgt tcgcaaaggc agcgccgccg caagaaggag acgcgtggga
     5281 gttgggagcg gcacgtcgag ttcatgatac aggttggcga attgcggcat ggcgtaggtt
     5341 acgatgtagg tgattaccag cagcactaca gccacgagaa ccgagggata aatcagcgca
     5401 gtcatcaacc tgcttcggaa tccggtactc acacgcaaga attcgatata ttgctccaac
     5461 acgccgatgt ggtttccgct gcgttcgccg gcagtgatca cggttacata cacggggggg
     5521 aatgagcctt gggcagcgag cgcctcagat aacagagcgc catccttaac tcgctggcgt
     5581 acttcgatca ggatcgggcg aatctggggg gctgcggcac gctcggccag gaggtccagc
     5641 gcgcgcaaaa tgggcaagcc tgcctttatt aaggtgtgaa actgctggtt gaaaatcaga
     5701 aagtggtcag ggcgaatctt gcgctcgggg cggccgcttc ctatctgcgc cagcaagtcg
     5761 aaatgactgc gcacagagaa aacataaaag ccgcgctcgg caagcttctg cctggcttcc
     5821 ccttccgact gggccgtttc aacctgttgg aagatccgtc cggaggtatc cccgattttg
     5881 caaacaaatt cagccatttt tgatcgctcg gcgcgccgcc gcaatccact agggccgagt
     5941 ttaacatagg cccgcgatct cgcagggaga tcgcaagtat tgtccgttat taagatgtga
     6001 cattttggaa aaggctgtat gggg
//
LOCUS       DATNDT010000115         4948 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3709730, whole genome shotgun sequence.
ACCESSION   DATNDT010000115 DATNDT010000000
VERSION     DATNDT010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3709730"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1251
                     /gene="dnaE"
                     /locus_tag="VN788_03610"
     CDS             <1..1251
                     /gene="dnaE"
                     /locus_tag="VN788_03610"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721943.1"
                     /note="catalyzes DNA-template-directed extension of the
                     3'-end of a DNA strand by one nucleotide at a time.
                     Proposed to be responsible for the synthesis of the
                     lagging strand. In the low GC gram positive bacteria this
                     enzyme is less processive and more error prone than its
                     counterpart in other bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXU19653.1"
                     /translation="VVKIFDLMEQFAGYGFNKSHSAAYAYLAYVTAYLKAHYPVEFMC
                     ALLTSETGNTAKVVKYINECRDMGIQVLPPDINSSDKDFTPAAGGIRFGLCAVKNVGG
                     GAVESVIQSRADKGPFHSIYEFCERVDLGAVNRRMIESLIKAGAMDTLEGNRAQLFGV
                     VEGAMEVGQRAWKDRQSGQAGLFGGAFDDETRPEPPLPALPAWTSREKLSGEKEMLGI
                     YVTGHPLDEFEDKVRDLATHDSSNVEGLEKGVDVALCGILTGILRRRNREGKLWASMQ
                     LEDRQGALEAMVFTTNYENLLTQLVEDKAVLVRGMILPEENAPPKISVQSIVALENAR
                     IDLPTLISIRIPLSANGGINRAEELRKLFERKHGATGVRLRLEKSRDFSVILDVAGAV
                     RPDKEFRAEVERICGPESFEVLAN"
     gene            1255..2181
                     /locus_tag="VN788_03615"
     CDS             1255..2181
                     /locus_tag="VN788_03615"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXU19654.1"
                     /translation="MSDEANPFPDDLGDELESGAEEIAAAESLESLAPEPEPTNPAWQ
                     RVQLARHSKRPHSLDYIQLIFNNFEELHGDRFFGDDPAIVGGFAWLDGKAIMVIGQQK
                     GRDTKQKLIRNFGMPKPEGYRKAMRLMHLAEKFNRPIVTLIDTMGAYPGIDAEERGQA
                     EAIAMNLREMSRLRVPVITVVIGEGGSGGALALGVANRVYMLENAWYSVISPESCAAI
                     IYRDAGKAARAAAALRLTADELLRLNLIDAVVPEPPEGAQTDRETAAALLKQTLQASL
                     AELRQLTAIDLIEDRYVKFRRMGSFFLSAAGA"
     gene            2178..2453
                     /locus_tag="VN788_03620"
     CDS             2178..2453
                     /locus_tag="VN788_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19655.1"
                     /translation="MRKRVWVLIGVAFALILITVLVKSSMNLAKYRVEVCIDFQGRNS
                     CRTAAAVTEADALRAAHDNACALVASGVTETIACEQIQPTKVRWIKR"
     gene            2697..4121
                     /locus_tag="VN788_03625"
     CDS             2697..4121
                     /locus_tag="VN788_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU19656.1"
                     /translation="MKKKWKILLIIGLVVVVGGGVFASIRYNQRGIVAVQTGRVVRQD
                     LTSLVTASGEIKPKNYYNLGANAMGPLTAINVKEGDQVRKGEVLAQIEAIQSKADVEA
                     QKAQIQTSLADSAAAEAGAKAMQDAIATAQAGLEKANAELERTKLNLDREAQLYKDKL
                     VAKQDYDQKKAEYDTAVAGILDANTRIAQAKSQEAQSRAQLVSAQRHVTQAQANLTRV
                     VDVFDKYSVISPIDGVVTNLPVRIGETVVPGIQSSTASTVMTVADMSLITAEVKVDET
                     DIVNVQLNQKAALTIDAIPDKTFHGHVIEIGNTAIVRSTGVAASQSQTSSQEAKDFKV
                     VIAMDDPPDDVRPGLSCTAKITTATRSNALTVPIQALTIRQKGDLEQQKKTGGVQAAT
                     KLTPAEEKARKQEIQGVFVIANGKAEFRKVDTGITGATDIEVLGGLKEGDEIVTGSYS
                     VIRTLRNEAKVKVDNKAPVKVEAT"
     gene            4124..4861
                     /locus_tag="VN788_03630"
     CDS             4124..4861
                     /locus_tag="VN788_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU19657.1"
                     /translation="MATATQELVDRGEQLRRDGIVIRTYDLWKTYIMGDQEIHAVSGV
                     DVQIRRGEYVAIMGPSGSGKSTLMNLIGCLDSPTKGLYYLNGRLASDTSDDELARIRN
                     KEIGFVFQTFNLLPRATALHNVELPLIYAGLTSDARLEKAKQALRAVDLEQRMFHRPN
                     ELSGGQRQRVAIARALVNNPSILLADEPTGALDTATGNEIMALFERLYQQGNTIVLVT
                     HEHDIALHAHRIIHIRDGKVERDETVK"
BASE COUNT         1105 a         1386 c         1509 g          948 t
ORIGIN      
        1 gtcgtcaaga tcttcgatct gatggagcag ttcgccggct acggttttaa taagtcgcac
       61 tccgcggctt acgcgtatct cgcgtacgtc acggcgtacc tcaaagccca ttacccggtt
      121 gaattcatgt gcgctctgct cacgtcggaa accggcaaca cggcgaaggt ggtcaagtac
      181 atcaatgagt gccgcgatat gggcattcag gttttgccgc ccgatatcaa ttcgagcgat
      241 aaggatttta cgccagccgc cggtggcatc cgcttcggac tctgcgcggt gaagaacgtg
      301 ggcggaggtg cggtggagtc ggtcatccag tcccgcgcgg ataaaggccc gttccactcc
      361 atctatgaat tctgcgagcg tgtcgatctc ggcgcggtca accggcgcat gatcgaaagc
      421 ctgatcaaag ccggcgcaat ggatacgctg gaaggcaatc gcgcccagtt gtttggtgtt
      481 gtggaaggcg cgatggaagt gggacagcgt gcctggaaag atcgccagag cggccaggcg
      541 ggtcttttcg gtggcgcttt cgacgatgaa acccgcccgg agcctccgct tccagcgctg
      601 ccggcgtgga cttcccggga aaaattgtcc ggcgaaaaag agatgctcgg catctacgtc
      661 accggccatc cgctcgatga atttgaagat aaggttcgcg accttgccac gcacgattcc
      721 agtaacgtcg agggcctcga aaaaggtgtg gacgtcgcgc tctgcggcat tctcaccggc
      781 attctgcggc ggcggaatcg cgaaggaaaa ctttgggcgt ccatgcaact ggaagaccgc
      841 caaggcgcgc tcgaagcgat ggtttttact actaattacg aaaatctact cactcagttg
      901 gttgaagata aagccgtctt ggtccgggga atgatcctgc cggaggagaa tgcgccgcct
      961 aaaatctcgg tccagagtat cgtggcgctg gaaaatgctc gcatcgattt gcctactctg
     1021 atttccatac ggattccgct cagcgcaaat ggtggcatta accgggccga agaactccgc
     1081 aagctcttcg aacggaagca cggcgccacg ggagtccggc tgcgtcttga aaagagccgg
     1141 gacttttccg ttattctgga tgtggccggc gccgttcgac ccgacaaaga gtttcgggct
     1201 gaggtcgaac gcatctgtgg gccggaatcg ttcgaggtcc tcgcaaactg aatcatgtcc
     1261 gatgaagcca atccatttcc ggacgaccta ggggatgagc tggagtccgg cgcggaagaa
     1321 attgccgctg cggaatcgct ggaatcgctc gcgcctgaac ccgagcccac gaatccggca
     1381 tggcagcggg tccaacttgc ccggcactcc aaacgcccgc attcgctcga ttacattcaa
     1441 cttatcttca ataacttcga ggagttgcac ggcgaccggt tctttggcga cgacccggcg
     1501 attgtaggcg gattcgcgtg gctggatggt aaagccatca tggtgatcgg ccagcagaaa
     1561 ggccgtgaca ccaagcagaa gttgattcgc aatttcggga tgccaaagcc cgaaggttac
     1621 cggaaggcga tgcggctgat gcatttggct gaaaagttca accggcccat cgtgacgctg
     1681 atcgacacga tgggcgctta tcccggtatc gacgccgaag aacgggggca ggctgaagcg
     1741 attgccatga accttcggga aatgtcccga ttgcgcgtgc ccgtgatcac cgtcgtaatc
     1801 ggggaaggcg gcagcggagg cgcgctggcg ctcggcgtcg ccaaccgagt ctacatgctc
     1861 gagaatgcct ggtatagcgt gatttcgccg gaaagttgcg ccgctatcat ctaccgggat
     1921 gcgggaaagg ctgcccgggc ggctgcggcc ctgcggctga ccgcggatga gttgctgagg
     1981 ctgaacctga tcgatgccgt agtccccgag ccgcccgaag gcgctcaaac ggatcgcgaa
     2041 accgcggcgg cgttgctcaa acaaactctt caggccagtc tcgcggagtt gcgccaatta
     2101 acagccattg atcttatcga ggaccgctac gtgaagttcc gccggatggg tagcttcttt
     2161 ctgtcggcgg ctggagcatg agaaagcgcg tctgggttct catcggcgtc gctttcgcgc
     2221 tgatcctgat tacggttctc gtgaaatcgt ccatgaatct ggcgaagtac cgcgtcgaag
     2281 tctgcattga tttccagggc aggaacagtt gccggaccgc ggctgccgtt accgaggcgg
     2341 atgcgctccg ggccgcgcac gataacgcct gcgcgttagt cgcttccgga gtcacggaga
     2401 caatcgcctg tgaacagatc cagccaacga aggtccgctg gatcaaaagg tgaccgtact
     2461 cggtggattt gggttcgttt tcgcattttc caagtcactg tataatctta ggcttagggc
     2521 aatccaatgg cgctccttcg tggaaggggc ctccccccgg gaaagccgtc tccagaacgt
     2581 ctgccctgtc tggcgaacgc tgagccgtaa cggtttgtaa gtaatcccgt gataacctgg
     2641 atttgacgaa caaaatcagg acaacttacg tctaagctgg acggagccgt gtattcatga
     2701 aaaagaagtg gaagatacta ctgatcattg gtttggtcgt agtggtaggt ggtggggttt
     2761 tcgccagcat tcgctacaat cagcgaggta ttgtggccgt tcaaacgggg cgcgtagtga
     2821 gacaggatct cacttccctg gtgacggcat ccggcgaaat caagccgaag aattattaca
     2881 acctgggcgc gaacgcgatg gggccgttga cggccatcaa tgtgaaggaa ggcgaccagg
     2941 tccgcaaggg cgaggtactg gcgcagatcg aggcgatcca gtcgaaagcc gacgtcgagg
     3001 cgcaaaaggc ccagatccag acgtcgttgg cggattcggc ggcagccgag gcgggcgcga
     3061 aggccatgca ggacgcaatc gccaccgctc aggccggcct tgaaaaggcc aacgccgagt
     3121 tggaacgcac caagctgaac ctcgaccgcg aggcgcagct ctacaaagac aaactggtgg
     3181 ccaagcagga ttacgaccag aaaaaggcgg agtacgatac tgcggtagcg ggcatcctgg
     3241 atgcgaacac tcgtattgcc caggcgaagt ctcaggaagc gcagtctcgc gcgcagctcg
     3301 tctcggcgca gcgccatgtg acccaggctc aggcgaacct gacgcgggtc gtcgacgttt
     3361 tcgacaaata ttccgtgatt tcgccgattg acggcgttgt cacgaatcta ccggtccgca
     3421 tcggcgagac cgtcgtgccc ggtatccaga gttcaaccgc cagcacggtc atgaccgtgg
     3481 ccgatatgtc gctcattacc gccgaggtga aggtggatga gactgacatc gtcaacgtcc
     3541 agctcaacca gaaagcggcg cttacgattg acgcgatccc cgacaagaca ttccatggcc
     3601 atgtgatcga gatcggaaac accgcaattg tccgctccac gggcgtcgcg gcatcgcaga
     3661 gccaaacatc tagccaggaa gccaaggatt tcaaggtcgt tattgcgatg gacgatccgc
     3721 cggacgatgt ccggcccggc ctgtcctgca cggcgaagat caccacggcg acgcgcagca
     3781 atgcgttgac ggttccgatc caggcgctga cgatccgtca gaaaggcgac ctcgaacaac
     3841 agaagaaaac cggcggagta caagcggcca cgaaactcac gccagccgaa gagaaggccc
     3901 ggaagcagga gatccaggga gtcttcgtaa tcgcgaacgg aaaggccgaa ttccgcaagg
     3961 tggatactgg catcaccggc gcgaccgaca ttgaggtgct cggcggtctg aaggaaggcg
     4021 acgaaatcgt caccggcagt tacagcgtga tccgcactct gcgaaacgaa gccaaggtga
     4081 aggtggataa caaggccccg gtgaaggtcg aggcgaccta acgatggcaa cagccaccca
     4141 ggaactcgtc gatcgcggcg aacagctcag gcgcgatggc atcgttatcc gtacctacga
     4201 tctatggaag acatacatca tgggcgatca ggagattcat gccgtttcag gcgttgacgt
     4261 ccagatccgc cgaggcgagt atgtcgcgat catgggcccg tccggctccg gtaaatcgac
     4321 gctgatgaac ctgatcggct gcctcgattc gcctacgaaa gggctgtatt acttgaacgg
     4381 cagacttgcc agcgatacca gcgacgacga gctggcgcga atccggaaca aagagattgg
     4441 gttcgttttc caaactttca atctgctgcc acgggcgacg gctctccaca atgttgagct
     4501 gcccctgatt tacgcggggc tgacatcgga cgcccgcctc gaaaaggcga agcaggcgct
     4561 gcgggccgtg gatttggagc aacgcatgtt tcaccgtccg aacgagctct cgggcggcca
     4621 gcggcagcgc gtcgccatcg cccgcgcgct ggtgaacaat ccctctatcc tgctggcaga
     4681 cgagccgacc ggcgctctcg acacggcaac cggcaacgag atcatggcgt tattcgagcg
     4741 gctctatcag cagggcaaca cgattgttct ggtcacgcac gaacacgata tcgcgctaca
     4801 cgcccatcgg attatccaca tccgcgatgg caaagtggag cgggacgaga cggttaagta
     4861 acgccccgac tccttctgtc atcatattcg acagaaggac gtgcaatgag agttgctcct
     4921 gcgcctgact ctggcgttcg cgctggtt
//
LOCUS       DATNDT010000116         4627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3486079, whole genome shotgun sequence.
ACCESSION   DATNDT010000116 DATNDT010000000
VERSION     DATNDT010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3486079"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..551
                     /locus_tag="VN788_03635"
     CDS             153..551
                     /locus_tag="VN788_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19658.1"
                     /translation="MRIKISMAVLVAVACLSLVESQTRTPRSIFGLYAPPAGRDSIRV
                     TRRRDGKIGVALKLYYANGHTCQIDKAGEWHGGRVLVIADGIDPNQPCKLEASFTNGH
                     ILLKDDGLRCAPVYCGTRGKLDGVSLPKKH"
     gene            561..1232
                     /locus_tag="VN788_03640"
     CDS             561..1232
                     /locus_tag="VN788_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19659.1"
                     /translation="MYPSVQAQFRAFNEPFEGSIPYMYLDVKGLVTVGVGNLIDPVAL
                     ATALAFTFKNKPGIATPGAAATPDQIAAEWQKLKSNTSLAQRGHLACAPITDLELADG
                     PLNDLILKRLTQNESFLKRQAPFAQFDTWPADAQLGLLSMAWAMGPAAPLNFHHFCAA
                     CQNLDFKTAAAQCEMSTAGNPGVIPRNNANQKLFQNAAAVLAGSADSGLQSATLYYPQ
                     ALASG"
     gene            1261..1803
                     /locus_tag="VN788_03645"
     CDS             1261..1803
                     /locus_tag="VN788_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19660.1"
                     /translation="MIKTLIKNWWLLAFCGVLDAMVSVVYFNHVGHGFHGLKAVVFLG
                     EVTLAAGVCTIAAGIWSSRNRKSWLLMLNGLACSALGLILTFWKGPLAFNTIALLIVV
                     MAISFGIYELATARTLRRLAGVVSVGFALVFLAFIFRWIELEPGSPGQTLLWLGSYFG
                     FSAICMLGLMRNNSTFAPQS"
     gene            complement(1914..3056)
                     /locus_tag="VN788_03650"
     CDS             complement(1914..3056)
                     /locus_tag="VN788_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU19661.1"
                     /translation="MRKLKAAIIGTGFMGKVHAEGIRRLGNVELAAVAGSSDARAKAF
                     ADSIGVERSTGDYKTILADPSIDAVHVLTPNALHFPMTKAALEAGKNVLCEKPLTVSS
                     AQAKELVDLAAKTGLANCVEHNLRYYPVVQQIHAMRENGDLGDILIVQGTYSQDWLLY
                     DTDWNWRIEEKANGPLRCMGDIGSHWMDMIQYLTGLKISSLCADLQTFFKERKRPKGS
                     VETFSGKKLKPEDYEPIHIDTDDFGSVLLRLGDRARGAFTVSQMSAGRKNRFAFEIFG
                     TKSGVSWNQECPDELWIGHRNSPNQVIVKDPSLLYPAAAAFADLPGGHSEGYDDSHKQ
                     VFKRFYARVADKSAPVEYPTFADGLLGMQLLEKVLESSRKQAWVKV"
     gene            3579..3926
                     /locus_tag="VN788_03655"
     CDS             3579..3926
                     /locus_tag="VN788_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19662.1"
                     /translation="MSVIRLAEAIQPRFEKTAEAVSVRFQPALMRGAVFTLGVASLVA
                     YLLRPLALAAAALGVWRLSADLGWTDAFFITHGLFSHFQVWFALAIGLHFVTVALDRS
                     VRPAAQQPPAEQS"
     gene            4335..4627
                     /locus_tag="VN788_03660"
                     /pseudo
     CDS             4335..4627
                     /locus_tag="VN788_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004627863.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          892 a         1349 c         1349 g         1037 t
ORIGIN      
        1 ctctctcgcg cgagttcgcc gccgcgcatc cgaacgtccg tctccacgag atgccctccg
       61 gccacgagct aaccaacgta ctggaaccga tgtgggaaac gacgaaatct tttttgcaat
      121 cggtggaatc gtgatacact cgcgcagaac tcgtgcgcat taaaatcagc atggccgtcc
      181 tggttgccgt ggcgtgtctc tctctagtgg aaagccagac gcgcacgccg cgatcgattt
      241 tcggactcta cgcgccgccc gccggacggg acagcatccg tgtcacgcgc cggcgcgatg
      301 gcaaaatcgg cgtcgctctc aagctctact atgcgaacgg ccatacctgc caaatcgaca
      361 aggcgggcga gtggcacggt ggccgtgtgc tcgtgatcgc cgatggaatc gacccaaacc
      421 agccgtgcaa gctggaagct tcatttacca acgggcatat tttgctcaag gacgacggcc
      481 tgcgctgcgc gccagtgtac tgcggaacgc gcggcaagct ggacggcgtc agtctgccca
      541 aaaaacatta ggagacccca atgtatccga gcgtccaggc gcagtttcgc gcgttcaacg
      601 agccctttga aggctcgatc ccctacatgt atctcgacgt gaaaggtctg gtgacggtgg
      661 gcgtcggcaa tctgatcgac cccgtagcgc ttgccaccgc gttggcgttt acgttcaaaa
      721 acaagccggg aatcgccacg cccggcgcgg ccgccacgcc cgatcagatc gccgcggagt
      781 ggcaaaagct caagagcaat accagcttgg cgcaacgcgg ccatctcgcc tgcgctccga
      841 tcacggacct ggagctcgcc gacggcccgt tgaacgatct gatcctgaag cgcctcaccc
      901 agaacgaaag ctttctaaag cgccaagcgc cgttcgcgca attcgacacg tggccggcgg
      961 acgcccaatt ggggctgctg agcatggcct gggcgatggg accggcggcc ccgctgaatt
     1021 tccatcattt ttgcgccgcg tgccaaaacc tggactttaa gacggcggcc gcgcaatgcg
     1081 agatgagtac cgccggaaat ccgggcgtga tcccacgcaa taatgcgaac cagaagctgt
     1141 tccaaaacgc cgccgccgtg ctcgccggga gcgccgattc gggccttcaa agcgctactt
     1201 tgtactatcc gcaagcgctt gcgagcggtt aagcgtatca tgatcatgga ggtttgctcc
     1261 atgatcaaaa cgctgattaa gaactggtgg ttactggcct tctgtggcgt tcttgacgcg
     1321 atggtttcgg tcgtctactt taatcacgta ggacacggat tccatgggtt gaaagcggtg
     1381 gtgtttctgg gtgaggtgac gctggcggcc ggcgtttgca cgatcgcagc cggcatttgg
     1441 agttccagaa accgcaaatc ctggcttctg atgctgaatg gccttgcttg cagcgcactc
     1501 ggactgatct taactttctg gaaaggtccg cttgccttca atactatcgc gctgttgatc
     1561 gtcgtaatgg caatcagctt cggcatttat gaattggcaa ccgcgcgaac tcttcggcgt
     1621 ctggccggag tggtctcggt tggttttgct ctggtgtttc ttgcttttat atttcgctgg
     1681 atcgaactgg agccagggtc gccgggccag actttgcttt ggctgggctc gtattttggt
     1741 tttagcgcta tctgcatgtt ggggctgatg cgcaataact ccacattcgc cccacagtcg
     1801 tgacgcaagc gtttgcgccg gcgttccggg cagcctaaac gcttgcttgc gcggctcgga
     1861 aatgcgctca cgcattagga aaaccctttc cgagccccga ccaaagggag cggtcagacc
     1921 ttcacccaag cttgcttccg ggagctttcc agcacttttt cgagcaactg cattccaagc
     1981 aagccgtcgg cgaaggtcgg atattcgact ggcgcggatt tatcggccac gcgcgcgtaa
     2041 aagcgcttga acacctgctt gtggctatcg tcgtagccct cgctgtggcc gcccggcaga
     2101 tcggcgaacg cggcggcggc gggatagagt agcgacggat ctttaacgat gacctggttc
     2161 gggctgttgc gatgcccgat ccacaattcg tcggggcact cctggttcca cgagacgccg
     2221 ctttttgtcc cgaaaatctc gaaggcgaac ctgttcttgc ggccggccga catctgactg
     2281 acggtgaatg cgccgcgcgc gcgatccccg agccgcaaga gaaccgagcc gaagtcgtcc
     2341 gtatcgatgt ggatcggttc gtaatcttcc ggtttgagtt ttttacccga aaaagtttcg
     2401 accgatcctt tcggccgctt ccgctccttg aagaaggttt gcaaatccgc gcacagcgag
     2461 ctaatcttca atcccgtcaa gtattggatc atgtccatcc agtgggagcc gatgtcgccc
     2521 atgcagcgca gcgggccgtt ggccttttcc tcgattcgcc agttccagtc ggtgtcatag
     2581 agcagccagt cctgcgaata cgttccctgc acgatcagga tgtcgccgag gtcgccgttt
     2641 tcacgcatgg cgtggatctg ctgaaccacc ggatagtagc ggagattatg ttccacgcag
     2701 ttcgcgaggc cagttttggc ggccagatcc accagctctt tcgcttgcgc ggaagagacg
     2761 gtcagcggct tctcgcagag cacgttcttg ccggcttcga gcgcggcctt ggtcatcggg
     2821 aaatgcagcg cgttgggcgt cagcacatgg acggcgtcaa tcgatgggtc ggcgagaatc
     2881 gttttgtaat cgcctgttga gcgctccacg ccaatgctgt ccgcgaatgc tttggcgcgc
     2941 gcgtcgctcg atccggccac ggcggcgagt tcgacatttc caagccgccg gatgccttcc
     3001 gcatgcacct tccccataaa tccggtgccg attatggcag cctttaattt ccgcatgttc
     3061 gagttccggt ccttttcaaa ttaaatttgg ttgcgattgg taaactgaga ttctacagga
     3121 atggtcgttc gaatacgact cggcagcggg tcaaaggtaa ggcggagcgg gcggcggaat
     3181 cagcggctcg cgttggctgc cgcggcgcta cttcagccgt tggtcgctat gtccggagcg
     3241 ctcggatgtt ggcggctcgg cgcagacctc ggcgcgttcc gcgcgttcgc aattccggct
     3301 ggcctgtttt ctcattcgca agtgtggatg tgcttggccg cggcgctcga agcttccgcg
     3361 atttctctga accggtatgg gcggaagcga ggcgtgtcag cgaaaaaagc gctaccaaaa
     3421 tcggaagaag tcactcctcc gatacgaaat cccggattta atgcttcggt accactttcc
     3481 gccgaaacta aacccgcaaa agtccgataa gactcactcg tagatccgaa accaacgcca
     3541 acatcgttgc atctatgtag gcaggcagga ggggccaaat gtcagtgatt cggcttgcgg
     3601 aggcgattca gccgcgattt gagaaaaccg ctgaggcggt atcggtgcgg tttcaacccg
     3661 ccctgatgcg cggcgccgtc ttcacgcttg gggtggcttc cctggttgcc tatctgcttc
     3721 ggccgctggc tttggccgcc gcggcattag gtgtgtggcg attgtcggcg gacctgggat
     3781 ggacagacgc gtttttcatc actcacgggt tgttctcgca ttttcaggtt tggttcgcgc
     3841 tcgccatcgg cctgcatttc gtaaccgtgg cactcgaccg ctccgtgcgg cccgccgctc
     3901 agcaaccgcc agccgagcaa agctaatcca cccattgacg ggatggtagt gctctagatg
     3961 tgcgtggctg attcatgctg tctccagcag ctcgcggact gtccagatgt ggccttgcgt
     4021 gtttttcgat gtcggctagt tctaaaacca tagttccaaa actataatgt tgccgaaaac
     4081 gcgagtcttg gagcgtggcc ttgccgctac cggaggcagt ttctccgcag gccaggccgt
     4141 tagctttgtc agctggtggg gtagactggc tcatgccact tcgtagaaaa tcgcgaactc
     4201 ctaaatccac gcaaacgacg caaagccact tcgcgaatgt caacggcgtg cggctgcact
     4261 atctggccgc tggcacgggc gacccagtgt acctcttgca tggttacgcc taaacgagcc
     4321 gcaggtggca tcccttgatt gcagacctcg caatcgccca catcatgata gccccggatt
     4381 tgcgcggcgc cggtcaatcg tccaccccat ccgctggcta cacgaaggcc gaaatggcgc
     4441 aagacatcca cgctcttgat tgggttgatg gctgcatacg cctatgcagc tcaatatccg
     4501 aacgaagtga atcggattgc gctcctggac gccttcttgc cgggaatagg aaactggcgc
     4561 gacgtatggc tgatgcggga tctttggtat ttccattttt atggcaagac gctgctcgcg
     4621 ctcgtag
//
LOCUS       DATNDT010000117         7051 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_326988, whole genome shotgun sequence.
ACCESSION   DATNDT010000117 DATNDT010000000
VERSION     DATNDT010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7051
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326988"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1259)
                     /locus_tag="VN788_03665"
     CDS             complement(<1..1259)
                     /locus_tag="VN788_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HXU19663.1"
                     /translation="MRRSLLIVSVLLLPLAFAQDTHYHIEGEQIPGPANPADFSTWLA
                     DISQWRVERLIRAGYDGSQYARPELQWAQHNFVQPQMMVEDRYFYDPAAGKYTVDRYL
                     DDVTKRYGGIDSVLIWHVYPNIGIDNRSQYDLLRDMPGGLAGVRQMVEDFHRHGVKVF
                     FPAMPWDRGSHDEGMPMWDATARIMAEVNADGINGDTFGGIPRAFRVASDKTGHPLVF
                     EPEGAPDDEALAWNNQSWGYWKYPSVPMISKQKWLERRHMVNVCDRWAHDHNDDLQSA
                     FFNGVGFESWENIWGIWNQMTPRDAEALRHIAVIYRKLGDLLVSDDWEPHTPTLQYGI
                     YASKFPGQTETLWTFINRNEYDVTGRQIRIPYRQGISYFDLWHGLELTPEIEKSGNEQ
                     TAVFRFAVGAHGYGALLATSRPDEFKA"
     gene            1264..2070
                     /locus_tag="VN788_03670"
     CDS             1264..2070
                     /locus_tag="VN788_03670"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HXU19664.1"
                     /translation="MKRFSKIVSVDAAKKDRGKIDRQKTGGAPKTLDRVLSKAGLGSR
                     TEARKWIGGGRVRVNGKLVQTPDHWVDLKNDRVTLDGKTVREREKEYLLLYKPKGYIT
                     TYKDPEGRPTVYDLFGDDREWMFPVGRLDLETSGLLLMTNDSQFAERITNPAFKVPKT
                     YLVKAADLLATEQIDALRTGVVLNDGPTQPAEVRRLRDQGKHTFIEMTIREGRNRQVR
                     RMIEAIGSKVLKLVRIRIGEIEIGNLEIGKHRELRPDEICGLLQYTKCKP"
     gene            2103..2843
                     /locus_tag="VN788_03675"
     CDS             2103..2843
                     /locus_tag="VN788_03675"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU19665.1"
                     /translation="MSAQSPSAPSSPGQALFRSNCAFCHGLTATGGRGPSLVTSELVR
                     AGTPDTLQKVIRGGVPGTSMPAFSDFTDAQLGQLVDFLKGLAVHTVKQGPIPGNAEHG
                     KQIYAANGCSGCHQINSSGSVFGPDLSRVGAARPAEYIQTSITDPSADVPIESEGVTV
                     VTNDGKQITGVLVNRDTFTVQLRDTSQRFRMFQNDQVREVRALTKSLMPPYQSFSATD
                     LQDLVKYLTTLRSPAEKSAVVTTEEGIK"
     gene            2844..4412
                     /locus_tag="VN788_03680"
     CDS             2844..4412
                     /locus_tag="VN788_03680"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU19666.1"
                     /translation="MKSILAILFVCAAGAAQPVSYDRIVNARKEPQNWLTYWGDYSAV
                     RYHDLKQINTANVKDLRVDWIFQTPQVGAFETVPLVVDGVMYFTTTNGLVYALDAMSG
                     RQLWMYQYPLKDPGGPNRGLAILGDRLFMTTPNAHLIALDSRNGALLWDVLAAPPKKG
                     YTATMAPLAIKDKILMGISGGELGIRGFIDAYDPKTGKRLWRFWTIPSKDEPGGDTWA
                     ADSWQRGGGATWMTGTYDPDLNTVYWGVGNPGPDLYGESRKGDNLYTASLVALDGDTG
                     KLKWYFQFSPHDTHDWDAAETPMLLNLTWKGQPRKVVVQANRNAFFYVLDRVTGEFLL
                     GKSFARQTWAKGLDAKGRPIPMPNIEPSADGTKLCPGLAGGANWMAPSYNPQTGLFYV
                     PYIEGCDTYFSAPPVYIEGKPFWGSVFRAQESERASGRLKALDPLTGEAKWTFPMYHQ
                     TWSGTLATSGGLVFAGDEDGYLMAFDAKTGKILWKLNTGNRLVTGPITYMVNGRQYIA
                     MPSGAALIAFALPR"
     gene            complement(4416..5375)
                     /locus_tag="VN788_03685"
     CDS             complement(4416..5375)
                     /locus_tag="VN788_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HXU19667.1"
                     /translation="MAENFKDLTERDILALAISLEEEDGRIYGDFAEGLRESYPATAK
                     MFEEMQAEESTHRARLIEMYRQRFGEHIPLIRRQDVKGFVERRPLWLIRPLGISTVRK
                     QAELMELETRRFYERAIQRVTDASTRKLLGDLADAERQHYAAAESLERKHVTPEVREQ
                     EETSQRRLFVLQIVQPGLAGLMDGSVSTLAPLFAAAFATHNSWDAFLVGLAASVGAGI
                     SMGFAEALSDDGSLTGRGQPVVRGVICGLMTTAGGIGHTLPFLIPNFHIAMTVAIIIV
                     AIELGAISYIRHRYMDTPFLSAAFQVVVGGVLVFIAGILIGSS"
     gene            complement(5382..5840)
                     /locus_tag="VN788_03690"
     CDS             complement(5382..5840)
                     /locus_tag="VN788_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXU19668.1"
                     /translation="MFHNLIDTRNAVLPTILRIVLGIVFFAHGAQKMLGWFGGFGFSG
                     TMGSFENQMHIPAAFAFLAICAEFFGGIGLIIGLLGRVAAFGITINMLVAIFMVHHRF
                     GLFMNWNGNQKGEGFEYHLLAICVALAIMVLGSGAISIDLLLSRGAARTA"
     gene            5891..>7051
                     /locus_tag="VN788_03695"
     CDS             5891..>7051
                     /locus_tag="VN788_03695"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU19669.1"
                     /translation="MVIAKAAAVLLMGAFLASAGEYEDLFQEAAALSRQGKFAQAITK
                     YKAALLLRPGAPEALNNLAVMYYQTREYGDAWETASRIWKNHPEMSSAALIAGLSAVQ
                     LKKPTDAIAPFEQALAESPNNRDAILGLAAAHLALNQLPEAAGLYERQAARAPGDADG
                     WYGLAICYERMAEGASRKLAKMPGGGFYSKRLLGEFLSASGDAQLAREAFGESAGAAT
                     DASPEAAAEYARARDLAGKSRDAFQHLVTAAPDSWQANLFFGDVERQHRNFQQALDHY
                     EKAARQKPENAAALLGIGTVHWEMGEFDQAEKSLKQALHLNPDAIQATFELANIAVRK
                     HQDDRAIPLLKKYLAQQPDAFAARADLGRAYLHLGQFALAAEQLGKATPADTQ"
BASE COUNT         1593 a         1962 c         2086 g         1410 t
ORIGIN      
        1 aaggccttga attcgtccgg gcggctggtg gcgagcaacg ctccgtaacc gtgtgcgccc
       61 acggcgaaac gaaacaccgc agtttgctcg ttgccggatt tctctatctc cggtgtcaat
      121 tccaagccgt gccagagatc aaaatacgaa atcccctggc gatatggaat acggatctgg
      181 cggcccgtca cgtcatactc gttgcgattg ataaacgtcc acaacgtttc tgtttggccg
      241 gggaacttgc tcgcataaat gccgtattgc agcgtgggcg tatgcggctc ccaatcgtca
      301 ctgacgagga gatcgcccaa tttgcgatag ataaccgcga tgtggcgcaa cgcctccgcg
      361 tcgcgcggcg tcatctgatt ccagatgccc cagatgtttt cccagctctc gaagcccacg
      421 ccgttgaaga aggcggattg gaggtcgtcg ttgtgatcgt gcgcccagcg atcacaaaca
      481 ttcaccatgt ggcggcgctc cagccatttc tgtttgctga tcatcgggac cgacgggtat
      541 ttccagtagc cccagctctg gttgttccag gccagcgctt catcgtccgg ggcgccttcc
      601 ggctcgaaga cgagcggatg gcccgtctta tccgaagcaa cgcggaaggc ccgtggaata
      661 ccgccaaacg tgtcgccgtt aattccgtcg gcgttcactt cggccatgat gcgcgccgtc
      721 gcatcccaca tcggcattcc ctcgtcgtgc gagccgcgat cccaaggcat ggcgggaaaa
      781 aagaccttca cgccgtgacg atgaaaatcc tccaccatct ggcgaactcc cgcgaggcca
      841 cccggcatat cgcgcagcag atcgtattgg ctgcgattgt cgattcctat attcgggtag
      901 acatgccaga tgaggaccga gtcgatgcct ccgtatcgct tggtgacgtc gtcaagatag
      961 cgatccaccg tgtacttgcc tgcggcgggg tcgtagaaat agcggtcttc caccatcatc
     1021 tgcggttgaa cgaaattatg ctgtgcccac tgaagctccg gccgggcata ctgcgatccg
     1081 tcgtaacccg cgcggatcag gcgctccacc cgccactggc tgatatcggc gagccatgtg
     1141 gaaaaatcgg ccggattcgc ggggccggga atttgctcgc cctctatgtg ataatgcgtg
     1201 tcctgcgcga atgcgagcgg gagcagcaga acggagacaa tgagaagtga tcggcgcatg
     1261 gttttgaaaa ggttctcaaa aatagtatca gtagatgcgg caaaaaagga ccgaggtaag
     1321 attgatcggc aaaagaccgg cggcgctcca aagacgctgg accgcgtgct ctccaaagcg
     1381 ggcctaggat cgcgcactga agcgagaaag tggattggcg gcgggcgcgt gcgcgtgaac
     1441 gggaagctgg tgcaaacgcc cgatcattgg gtggatctta aaaacgaccg cgtcaccctg
     1501 gacggaaaga cggtgcgcga acgtgagaaa gagtatctgc tgctctataa gccgaaaggt
     1561 tacatcacaa catacaagga cccggaagga aggccaacgg tctacgacct cttcggggat
     1621 gatcgcgagt ggatgtttcc ggttggccgg ctcgatctcg agaccagcgg attgcttctg
     1681 atgaccaacg attcgcagtt cgccgagcgt atcaccaacc ccgctttcaa agtccctaaa
     1741 acgtatctgg tgaaggcggc cgatctgctc gcaactgaac aaatcgatgc gctgaggacg
     1801 ggtgtcgtat taaacgacgg accaacgcaa cccgccgaag tgcggcggct gcgcgatcag
     1861 ggtaagcaca cattcattga aatgacgatt cgcgaaggac gcaaccggca agtccgcaga
     1921 atgatcgagg caatcggcag caaggtcctt aaattggtcc gcatacgtat tggagagatc
     1981 gaaatcggca atctggagat cggaaaacac cgtgaactac gcccggatga gatatgcggg
     2041 ctactgcaat atactaagtg taagccgtga agtttatatt accgttactt ctcgctatat
     2101 ctgtgtccgc gcagagccca tccgcgcctt cgtcgcccgg ccaggcttta tttcgaagca
     2161 attgcgcttt ttgccatggc cttacggcaa ccggcggaag aggtcccagc ctggttacaa
     2221 gcgagttggt gcgcgccggc actccggata cgttgcagaa agtaattcgc ggcggcgtcc
     2281 ccggtacgtc gatgcctgca ttttcggact tcaccgacgc gcaactcggg cagctcgtcg
     2341 actttctgaa gggtttggct gtacacaccg tcaaacaggg cccgataccc ggcaatgcgg
     2401 aacacggaaa gcagatttac gcggcaaacg gctgttcggg gtgccaccaa atcaatagca
     2461 gtgggagcgt attcgggccg gatttgagcc gggtcggcgc ggctcgcccg gctgaatata
     2521 tacaaacgtc aattaccgat ccttccgcgg atgttccgat cgaatccgaa ggcgtgacgg
     2581 tggttaccaa cgacgggaaa cagatcactg gagtgttagt caatcgggac acgttcacgg
     2641 tgcagttgcg ggatacatcc caacgattcc ggatgtttca gaacgatcag gtgcgagagg
     2701 tcagagcatt aaccaaatcc ctgatgccgc cgtaccaatc tttttccgcc acggatttac
     2761 aggatcttgt gaaatatctg accactctgc gcagccccgc cgaaaagagc gccgtggtca
     2821 cgacggaaga agggatcaag taagtgaaga gtatcctggc gatactattt gtctgcgccg
     2881 ccggagccgc gcaaccagta agttacgacc ggatcgtgaa cgctcgaaag gaaccccaaa
     2941 actggctgac ttattggggc gattattccg cggtccgcta ccacgatctg aaacagatca
     3001 atacagccaa cgtgaaggat ctccgggtcg attggatctt ccagactccc caggttggcg
     3061 cctttgaaac ggtaccgctg gtcgtcgatg gcgtaatgta tttcaccacc accaatgggc
     3121 ttgtgtacgc gcttgacgcg atgagcgggc gccagttgtg gatgtatcaa tatcctttga
     3181 aggatcctgg aggcccgaat cgtggcctcg cgatactggg cgaccggctt tttatgacga
     3241 cgccgaacgc acacctgatc gctctcgatt cgaggaacgg ggctttactt tgggacgtgc
     3301 tggcggcccc gcctaagaag gggtatacgg ctacaatggc tcccctcgct ataaaagaca
     3361 agatcctgat gggaattagc ggcggcgaat tgggaatccg cggattcatc gacgcttacg
     3421 acccgaaaac cggcaaacgc ctatggcgat tttggacaat cccatcgaag gatgagccgg
     3481 gtggcgatac gtgggcggcc gattcctggc agcggggcgg cggcgccacc tggatgaccg
     3541 gcacttacga tccagatttg aatacggtgt actggggtgt cgggaatccc gggccggacc
     3601 tctatggtga atcccgaaag ggcgacaact tgtacaccgc atcgctggtg gctctcgatg
     3661 gagacacggg aaaactgaaa tggtatttcc aattctctcc tcacgacacg catgattggg
     3721 acgcggccga gacgccaatg ctgctgaacc tgacgtggaa gggacagccg cgcaaagtag
     3781 tggtccaggc caaccggaac gctttcttct atgtgcttga ccgtgtgacg ggcgagttcc
     3841 ttctggggaa gagcttcgcg agacagacgt gggcaaaggg attggatgcg aaaggccgtc
     3901 cgattccgat gccgaacatc gagccttccg cggacggcac taagttatgt ccggggcttg
     3961 ccggaggagc taactggatg gcgccatctt acaatcccca aacggggtta ttctatgttc
     4021 cttatatcga aggctgcgat acttactttt cagcgccccc ggtgtatata gagggaaagc
     4081 ccttctgggg cagcgtattt cgggcgcagg aaagcgagag agcctcgggg agattaaagg
     4141 ctctcgatcc gttaaccggg gaggcgaagt ggacgttccc catgtatcat cagacctggt
     4201 ctggaaccct ggccacgtca ggcggccttg tattcgcggg agatgaggac gggtacttga
     4261 tggctttcga tgcgaagacc ggaaagatac tgtggaagct gaataccggc aaccgtcttg
     4321 tgactggccc gatcacttac atggtaaatg gccggcaata tattgcgatg ccgtccggcg
     4381 ccgcactaat cgctttcgcc ttgccgcggt agttactaag aactgccgat caagataccg
     4441 gcaataaaga caagcactcc gccgaccacc acctgaaacg ccgccgatag aaatggcgtg
     4501 tccatgtagc ggtgcctgat gtagctgatg gctcctagct caatcgccac gataataata
     4561 gccacggtca tcgcgatatg aaaattcggg atgagaaacg gaagcgtgtg gccgatccct
     4621 ccggcggtgg tcattaatcc gcaaatcact ccgcgaacaa ccggctggcc gcgcccggtg
     4681 agcgagccat cgtcggagag cgcttcggcg aatcccatgg agataccggc tccgaccgac
     4741 gccgccagtc ccaccagaaa cgcgtcccag gaattatgtg ttgcaaaggc ggcggcgaac
     4801 agcggcgcga gcgtggagac ggacccatcc atcaggcccg cgaggccggg ttgcacaatt
     4861 tggagaacga aaaggcggcg ctgggaagtc tcctcctgct cgcgcacctc gggcgttacg
     4921 tgcttgcgtt cgagcgattc cgccgctgca tagtgctggc gctcggcgtc ggccaaatct
     4981 cccagcagct tccgcgtgga tgcatcggta acgcgctgaa ttgcacgttc ataaaatcgg
     5041 cgcgtctcca gttccattag ttcggcctgc ttgcggaccg tactgatgcc aagcggacga
     5101 atcagccata ggggccgccg ttcgacgaat cctttgacgt cctggcggcg gatcagcggg
     5161 atgtgctctc caaatcgctg gcggtacatc tcgatcagcc gcgcgcgatg cgtcgactct
     5221 tcggcctgca tctcctcgaa cattttcgcc gtggcgggat acgattcacg aaggccctcg
     5281 gcgaaatcgc cgtaaatgcg gccgtcttct tcttcgagag agattgccag cgcgaggata
     5341 tcgcgctctg tcaggtcctt gaaattttcc gccatcggcc gctacgcggt tctggccgcg
     5401 ccccgcgaaa gcagcagatc aatcgatatt gccccggaac cgagcaccat gatcgccagc
     5461 gcgacgcaaa tagcgagcag atgatattcg aagccttcgc ccttctggtt gccgttccaa
     5521 ttcatgaata agccgaagcg gtgatgaacc atgaaaatcg cgaccagcat gttgatcgtg
     5581 attccaaagg cggcaactcg gccaagcaat ccaatgatga ggccgatgcc gccgaagaac
     5641 tccgcgcaga tcgcgagaaa cgcgaatgcc gccgggatgt gcatctggtt ttcgaaagac
     5701 cccatggtgc cggaaaatcc gaagccgccg aaccaaccaa gcattttctg tgctccatgc
     5761 gcgaagaaaa cgatgccgag aacaatgcgt agaatggtgg ggagaacggc attgcgagtg
     5821 tcgatcaggt tgtgaaacat aaggctcctt ctgctggata ttacctcacg ctgctacact
     5881 tccgttggga atggttatcg cgaaggccgc cgctgttctg ctgatgggag cgtttttagc
     5941 cagcgccggc gaatacgaag atctctttca ggaagcggcg gcgctcagcc ggcagggcaa
     6001 gttcgctcag gccattacga aatacaaagc ggctctgctg ctgcgccctg gagcgccgga
     6061 agcgctgaac aatctagcgg taatgtacta tcagacacgc gagtatggcg acgcgtggga
     6121 aactgcatca cggatctgga agaatcatcc tgaaatgtct tccgccgcgc tgatcgcggg
     6181 gttatcggcg gtacaactca agaagccgac tgacgcgatt gcgccattcg agcaggcact
     6241 cgcggaaagt ccgaataatc gtgacgcaat cctgggcctt gcggccgctc atcttgcgtt
     6301 gaaccaactt cccgaagcag cgggactcta cgaacgacag gctgcgcgcg cgccgggtga
     6361 tgccgatggc tggtacggtc tcgcgatctg ctatgaacgg atggctgaag gcgcttcgcg
     6421 caagctggcg aaaatgccag gtggcgggtt ctattcgaaa cggttacttg gagaatttct
     6481 atccgcgagc ggagatgctc aactggcgcg cgaagccttt ggcgaatcgg cgggcgcggc
     6541 tacggatgct tcgcccgaag cggcggcgga atacgcgcga gcccgcgatc tggcgggaaa
     6601 atcgcgggac gcgtttcaac atctggtcac cgctgcgccc gattcgtggc aggccaatct
     6661 gtttttcgga gacgtggagc ggcagcatcg aaatttccaa caggcgctgg atcactatga
     6721 aaaagccgcc cgccagaaac cggaaaacgc cgcggccttg ttgggaattg ggacggttca
     6781 ctgggagatg ggtgagttcg atcaggcgga aaagtcgctc aagcaagcgc tgcatctgaa
     6841 ccccgatgcg atccaagcca cctttgagtt ggccaacatt gccgtgcgaa agcatcagga
     6901 cgatcgtgcc attccgttac ttaaaaaata tctggctcaa caaccggatg catttgccgc
     6961 gcgcgcagat ctgggccggg cttacttgca tctcggccag ttcgccctgg cggccgagca
     7021 actcggcaaa gcgacgccgg ccgatacgca a
//
LOCUS       DATNDT010000118        10221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1792393, whole genome shotgun sequence.
ACCESSION   DATNDT010000118 DATNDT010000000
VERSION     DATNDT010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792393"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="VN788_03700"
     CDS             complement(<1..592)
                     /locus_tag="VN788_03700"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HXU19670.1"
                     /translation="MTAATKKARLALVKPNKHVESLPVLRTIDLFSGAGGITEGFRQA
                     GYACLYANDCMTEAVETFRQNHPEAWADCRNIEHVKPSEIRSKLKLAKGQIDVLVGGP
                     PCQGFSINAPERFLADPRNKLFKDYVRFLEEFQPKTFLFENVPGLLSLGDGKVLDHIL
                     SEFTRLDYHVAIKILFAAHYGVPQERWRLILLGSRFG"
     gene            complement(589..816)
                     /locus_tag="VN788_03705"
     CDS             complement(589..816)
                     /locus_tag="VN788_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011829974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HXU19671.1"
                     /translation="MTRGAKRKRERKPGPTEGTSVRATISFPSELYKTLEGIAREKKV
                     SLAWVVREASEQYIVDKWPLFGKGLIGGEKQ"
     gene            complement(1411..2016)
                     /locus_tag="VN788_03710"
     CDS             complement(1411..2016)
                     /locus_tag="VN788_03710"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXU19672.1"
                     /translation="MKTSNDEQRILDAAHRHLRVAIVLLVQTGGRTYSEGLSLRWDQI
                     DLVHSVIHLDGAVKTSDSAQPLPLSWLACDVLKEWKKEQGSDSPYVLPSPRTADKPIR
                     SVKHAWRTTLKNAGVSYFPISNLRHVFCTRLSWVAADAVVQRAMRHSSPETKRHYQLG
                     MVDQVREGIELANEPAHHGKQELLRFYYGSAKAEKKGSRAS"
     gene            complement(2224..2463)
                     /locus_tag="VN788_03715"
     CDS             complement(2224..2463)
                     /locus_tag="VN788_03715"
                     /inference="COORDINATES: protein motif:HMM:NF024988.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXU19673.1"
                     /translation="MAILTVLAEAEEAEFCCVEQISGLTKGNLSAHVSRLEEAGYLTV
                     RKFFRAKIPATALTITVLGRKALRGYRKQLRAALR"
     gene            complement(2749..4131)
                     /gene="zwf"
                     /locus_tag="VN788_03720"
     CDS             complement(2749..4131)
                     /gene="zwf"
                     /locus_tag="VN788_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXU19674.1"
                     /translation="MTNFDSDAFVFFGATGDLAYKQIFPALQAMILRDHFDMPIIGVA
                     RSAKGLDGLRARAHESVEKHGGVDSRAFATLSAKLQFVNGDYGNQETFTNLRKALGGA
                     SHPLYYLAIPPDMFATVAEGLAKAGCDKGARVVVEKPFGRDLASAQALNRTLRKSFPD
                     SAIFRIDHYLGKEPVQNLLYFRFANSFLEPIWNNNYIQSVQVTMAEALGVEGRGGFYE
                     EVGAIRDVVQNHMLEVVALLTMEAPIARDLDALRDEKQRAFRAMRPVALADVVRGQFR
                     GYRNEKGVAPDSQVETFAAVKLYIDTWRWAGVPFYIRAGKRMPVTATEVLVELKRPPQ
                     AVFDSITQSQADCFRFRLSPSVTISLGARVKMPGEAMVGEAVELVVRQSPADEMTPYE
                     RLLGDAIRGDATLFVREDGVEAAWAVVDPILGNAVPVHDYEPNTWGPAEADQIIARDD
                     GWHNPKPFSV"
     gene            complement(4230..4925)
                     /locus_tag="VN788_03725"
     CDS             complement(4230..4925)
                     /locus_tag="VN788_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HXU19675.1"
                     /translation="MKAAFKKANAVLVIDVGGTHVKVLATGQREEREIPSGSAMTASK
                     MVRDVKRVAKDWKYDHVSIGYPGPVLHGRPLHEPYNLGGGWVGFDFGKAFRCPVKVIN
                     DAAMQALGSYKRGRMLFLGLGAGLGSAMIVDGVVEPMELAHLPYKKGKTYEDYVGVRG
                     LKRLGKKKWRRHVIDVVERLKSALEAEYVVLGGGNAKSMKKLPPGIRLGDNRNAFVGG
                     FRLWKEKIKRRRG"
     gene            complement(4922..5935)
                     /gene="gnd"
                     /locus_tag="VN788_03730"
     CDS             complement(4922..5935)
                     /gene="gnd"
                     /locus_tag="VN788_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011491942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HXU19676.1"
                     /translation="MQLGMIGLGRMGSNMVQRLRHGGHDCVVFDVNSSSVEKLVSEGA
                     TGAASLDNFVAKLKPPRAAWLMVPAAVVDETLHELAGRMQSGDTIIDGGNSYYVDDIR
                     RASELKPKSIHYVDVGTSGGVWGSERGYCLMIGGEPDVVQRLNPIFATLAPGIGTATR
                     TPGRDKTDGTAEQGYLHCGPSGAGHFVKMVHNGIEYGIMAAYAEGLNILHHANAGTKK
                     VEVNAETTPLRQPELYQYELCLPDVAEVWRRGSVIASWLLDLTAAELLKDPSLKSYRG
                     RVSDSGEGRWTIKAAIDEAVPASVLSIALYERFSSRGDADFADRVLSAMRHEFGGHVE
                     KKA"
     gene            complement(6019..6384)
                     /locus_tag="VN788_03735"
                     /pseudo
     CDS             complement(6019..6384)
                     /locus_tag="VN788_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018507016.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
     gene            complement(6430..7170)
                     /locus_tag="VN788_03740"
     CDS             complement(6430..7170)
                     /locus_tag="VN788_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003634929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXU19677.1"
                     /translation="MSTAAASPIFMSKLKDRKEVAERTIAFRFEKPSGWTFKAGQFID
                     MTLLDPSETDSEGNTRGFSIASGPHEEMLMVATRIRDTAFKRVLKTMPIGTAVKIEGP
                     SGDLTLPNNFKRAVALLAGGIGITPFRSMLVRAAKEKLPHRIFLFYSNRRPEDAPFLE
                     ELQTLERANPNYKLIASMTEMKKSQRPWDGETGLINPEMLTRHLKGAGTPIYFIAGPP
                     AMVKGLHEMLNKAGVDDDDIRAEEFAGY"
     gene            complement(7213..9681)
                     /locus_tag="VN788_03745"
     CDS             complement(7213..9681)
                     /locus_tag="VN788_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXU19678.1"
                     /translation="MKAQPARIARSKAGNTLQSIAVGDRKKSTKKDSAFGRPGIEPRW
                     THGDKDGVGTSYAASSRIWFTLWNGVITEVYYPTVDHPQVRDLQFLITDGKTFFHEER
                     RDLKSKCERLSDHALGYRVTNLDPNGHYTVIKEIITDPHLACILQRVHLGGDDAFISK
                     LRLYTLCAPHLQVGGWGNNGYAIEAAGRNILMARKKGIWMALGGTVQFSRVSCGYVGR
                     SDGWTDLASNFQMDWEFDDAPDGNVALTGELDLEGNREFTMGLAFGDTEHCAITNLFQ
                     ALGIPFNEQLKRYTEQWDRTSSHLLPLEKCSSDKGNLYRSSFSLLLAHEDKSYPGALI
                     ASLSIPWGEAKGDADHGGYHLVWTRDMVSSASAMLAAGDRVTPLRALIYLAVSQQQDG
                     GFAQNFWVDGGPFWRGIQLDEVAFPILLAWQLQREDALQDFDPYPMVLKAAAYLMRHG
                     PVTQQERWEEASGYSPSTLASNIAALIGAACFARDRGDEAVATLLEDYADFLECHIES
                     WTVTTEGSLVPGIKRHYIRILPEEVDNANPLEDPNSKVLKIKNHPAGAQDSFPARGIV
                     DAGFLQLVRYGIRRPDDPLILDSLKVIDAVLKVDTSVGPVWHRYNHDGYGQREDGGPY
                     IGWGKGRAWPLLTGERAHYELSVGHDVKPFIRAIEGFASTTGLLPEQVWDEPDRPEAY
                     MFLGRPTGSAMPLMWAHAEYIKLLRSVSDSRVFDLIPEVATRYLGDRKARQLFEIWKP
                     NRQARSVKRGYTLRIQVPAAFSLHWSANDWKSAEDSPSSAQVLGFHIVDIPISNAQRP
                     PIRFTFFWTVENRWEGRDYVVAVE"
     gene            complement(9807..>10221)
                     /locus_tag="VN788_03750"
     CDS             complement(9807..>10221)
                     /locus_tag="VN788_03750"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXU19679.1"
                     /translation="SDDHLIAHTEVPGFSPHELKVSLESRWLRIGGRAELSGNRNAGG
                     TVMYSLEHSLLPAEQIFHVAELPVEVDPSKAKVTFKEGTLEIVMPKPSLAKNAPAQTN
                     LRPHVDSYSSDPTATVADVAAKGTGNKGRAVSSAR"
BASE COUNT         2205 a         2980 c         2658 g         2378 t
ORIGIN      
        1 cgccgaacct ggagccaagc agtatcaaac gccagcgttc ctggggcacg ccgtaatgtg
       61 ccgcgaacag tattttgatg gcgacatggt agtcgagccg cgtgaattcg ctaagtatat
      121 ggtcaagaac cttgccgtcg cccagcgaga gtaggccggg gacgttctca aagagaaagg
      181 tcttgggctg aaactcctcc agaaatcgca cataatcctt aaaaagtttg ttcctggggt
      241 ccgcgaggaa acgctcggga gcgttgatgg agaaaccttg gcaaggaggc cctccgacaa
      301 ggacatcgat ctgccccttg gccagtttga gctttgagcg gatttcagaa ggcttgacgt
      361 gttcgatgtt gcggcagtcg gcccaagcct ccggatgatt ctgcctgaaa gtctcaacgg
      421 cctcggtcat gcagtcgttc gcataaaggc acgcatatcc cgcttggcgg aatccttccg
      481 tgatgccccc tgccccggag aacagatcga tggtgcgcag tacgggcaag gactcaacat
      541 gtttattcgg cttcacaagt gcaagcctgg ctttttttgt tgctgcggtc attgtttctc
      601 gccgcctatc aggccttttc caaacagcgg ccatttgtcc acgatgtact gttcggacgc
      661 ttcgcggacg acccaggcca gcgagacttt tttctcgcgc gcgatgcctt cgagagtctt
      721 gtaaagttca gacgggaaac tgatggttgc tcggacagaa gtgccctcgg tcggcccagg
      781 ttttcgttct cgttttcgtt ttgcgcctct ggtcacttgg gttcgctcac ttgccgggga
      841 gtatagcacc gcaccacaat tcaccatagc ggtgaattct gtggaaagcc ttaaaatatg
      901 cgttagataa ccccgcacag cgcgattcct aacctcatgg actttgaaac ctctggctca
      961 cgcctgtgtc tcggagaatc ctagcatatt cgctttatat tcgcccatta atcctccttg
     1021 cggatacata tttgggcagc cccggccctt tttcaagtta gttattacaa aggctaatac
     1081 agctgtgcca tcacagcaga gggggtgtac gaccaggcgg cacggcgaac cattgcgaaa
     1141 ctgagagcca atgccaacga attcgggtac ccagcgaccc gccaaaaaca acatacattt
     1201 gccgcgcggt gcaacttcaa aaaatctaat accggaatgc tcgggacaac ttcgggacat
     1261 ttgagagagc gagaatctcg ttgattttct gtgtaagcga cgccagacaa agcatttagt
     1321 ggtttatccc caccttgttt gcaagcaggg ggtcgccggt tcgatcccgg ccacgtccac
     1381 caactatctt cctgcaattt caatgattta ctaggaggct cggcttcctt ttttttcagc
     1441 tttggccgaa ccgtagtaaa aacgtagtag ttcttgtttt ccgtgatggg ctggttcgtt
     1501 ggctagttca atgccctcgc ggacctgatc aaccattccg agttgataat ggcgtttcgt
     1561 ttctgggctg ctgtgccgca tggcccgctg caccaccgca tctgccgcga cccaactgag
     1621 gcgcgtgcag aatacgtggc gcaggttgga gatcgggaaa tacgatacgc cagcattttt
     1681 cagagtcgtt cgccatgcgt gtttgacact gcggataggc ttgtccgccg ttcgcggact
     1741 cggaagcacg taggggctgt ccgagccttg ttctttcttc cactccttga gaacgtcgca
     1801 agccaaccag ctcaatggta acggttgagc cgaatcgcta gtcttcacgg cgccatcaag
     1861 atgaatcacg ctgtggacga gatcaatttg gtcccatcga agcgataatc cctcactgta
     1921 ggttcgacca ccggtctgaa cgagcagcac aatcgccact cgaaggtgcc tgtgcgccgc
     1981 gtccagaatc cgttgctcgt cgttgctcgt cttcaacagt aagcatcctc ttgagtggcc
     2041 gggcgaatct gcaggtcttt gaacttgtcc aagacggagc ggcggtactc aagacggccg
     2101 caaggcttcc gagattcaat cctctgttgg catgcgtatc taatgacctc tcccgtcgac
     2161 cagtgcctca cgcggagacc atttccatta ataatgcctt ctaatataat aacctctcat
     2221 ttgtcatcga agtgccgccc tcaattgttt acggtaacct ctcagcgcct tgcgtccaag
     2281 aactgtgatt gtgagcgcag ttgcaggaat ttttgcgcgg aagaactttc taacggtaag
     2341 atagcctgcc tcctctaacc tcgaaacatg agctgacaaa tttcccttcg tgagcccaga
     2401 tatttgttcg acacagcaaa actcagcttc ttcagcctcg gccagcaccg tcaagattgc
     2461 cagcctcgcg ggttagtgca cgacacggtc cactccaaga actgcgtgaa cgtcgacttt
     2521 cagctcggat tccttggggc acatatttgc cgcctcagct tcataaattt cgcgtgctga
     2581 acgattccct ccagaaccat cacgagagcg attacatgtc ccggccattg aacaccattc
     2641 gcaacggaag gtccaaacgc tcgtccgctc aaaagaagcg cgcagatacc cagaactaaa
     2701 agggcagcgc aaaactcata tggagcgcga gcaaacaacc atatatagtc aaactgaaaa
     2761 gggcttggga ttgtgccagc catcatcacg agcaatgatt tggtcagctt cggcgggtcc
     2821 ccaggtattt ggctcgtagt cgtgaacggg aaccgcattt cccaggatcg gatccacaac
     2881 tgcccacgca gcttccacgc cgtcctcacg aacgaacaag gtggcatcac ctcggatggc
     2941 atcgcccagc agacgctcgt acggcgtcat ctcatcggct gggctttgtc gcacgacaag
     3001 ttccacagct tcgccgacca tcgcctcgcc cggcattttg acgcgcgcgc ccagcgagat
     3061 tgttacactg gggctgagac gaaagcgaaa acaatccgcc tgactctgag taattgagtc
     3121 gaacacggcc tgcggaggac gcttgagttc aaccaatact tcagttgcgg tgacaggcat
     3181 ccgcttacca gctcgaatgt agaatggaac gcctgcccat cgccatgtgt cgatatacaa
     3241 cttgaccgca gcaaatgtct ccacctgcga atccggagcg acacctttct catttcggta
     3301 gccacgaaac tgaccgcgga cgacatcggc aagagcgacc ggccgcatcg ctcgaaaagc
     3361 ccgctgcttt tcgtctcgca acgcgtccag atcgcgggcg atgggtgcct ccatcgtgag
     3421 cagcgcgact acctccagca tgtggttttg caccacgtca cggatcgcac ctacctcttc
     3481 gtagaagcct ccgcgaccct ctactccaag agcctcggcc atggtgactt gaacgctctg
     3541 gatatagttg ttgttccaga tcggctcgag gaacgaatta gcgaagcgga agtagagcag
     3601 attctggacc ggttctttgc cgaggtaatg atcgatccgg aaaatcgccg aatcgggaaa
     3661 ggatttgcgg agcgttcgat tgagcgcctg cgcggaggcg aggtcacggc caaacggctt
     3721 ttcgacaacg acacgcgctc ccttgtcgca gcctgccttg gccagtccct cggcaacggt
     3781 tgcaaacatg tctggcggga tggcaaggta ataaagagga tgagacgctc cgcccagcgc
     3841 ctttcgcaga ttggtgaatg tctcctggtt gccgtaatct ccgttcacaa actggagttt
     3901 tgccgatagc gttgcaaacg cgcgcgaatc cacgccgccg tgcttctcga cgctttcgtg
     3961 agcacgggct cgaagcccgt caaggccctt cgcagacctg gccaccccga tgatcggcat
     4021 atcgaagtga tcgcgcagga tcatcgcttg aagcgcaggg aaaatctgct tgtaggccaa
     4081 gtcccccgtc gcaccgaaga aaacaaacgc gtccgaatcg aagttggtca ttttccaccg
     4141 gtcctgttca cacttacttc gctgaggcct tctccacgtg tccaacccac gacctatcgt
     4201 cgtatcggct tgacgcacgc actagaaaac tagccacgcc gacgtttaat cttttccttc
     4261 catagacgaa agccgcctac aaacgcgttc cggttgtcgc ccagtcgaat gcccggaggt
     4321 agcttcttca tgcttttggc atttcctccg cccagcacca cgtattcggc ctctagcgcg
     4381 ctcttgagcc gttccacgac gtcgatcaca tgacgccgcc acttcttttt ccccagccgc
     4441 ttcaggccgc gaacaccaac gtagtcctcg taagtcttcc ccttcttgta aggaaggtgc
     4501 gccaactcca tcggctcaac taccccatcg acgatcatgg ccgatcctag cccagcaccg
     4561 agacccaaaa agagcatccg gcccctttta tagcttccaa gcgcctgcat ggcagcatca
     4621 ttgatgacct tgaccggaca cctgaatgcc ttgccgaaat caaatccgac ccagccgccg
     4681 cctagattgt aaggctcgtg gagggggcgt ccatggagca cgggtccggg atagccgatt
     4741 gagacgtggt catatttcca gtccttagcg acgcgcttaa cgtctcgcac catcttgctt
     4801 gcggtcatgg ctgagccaga gggaatctca cgttcctcgc gttgtccggt tgccaacacc
     4861 ttgacgtgcg ttcctcccac atcgatcacc aggactgcat tggccttctt gaaggcagct
     4921 ttcatgcctt cttctcgacg tgtccaccga actcgtgtcg catcgcggac agaaccctgt
     4981 cggcaaagtc cgcatcgccg cgtgaactga aacgttcata aagcgcgatg cttaggacag
     5041 acgcaggaac cgcttcatca atcgcggcct tgatggtcca gcgtccttcg cctgaatctg
     5101 acacgcgtcc tcggtaactc ttcaggctcg gatccttcaa tagctcggcc gccgtaaggt
     5161 ccaagagcca tgacgcaatc acgctgccgc ggcgccagac ttcagcgacg tcgggcaagc
     5221 acaactcata ttggtataat tctggttgcc gtagcggcgt ggtctcggca ttgacttcaa
     5281 ctttcttcgt gccggcattg gcgtgatgca agatgttcag gccctcggca taagccgcca
     5341 tgatgccgta ctcgattccg ttgtggacca tcttgacgaa atgaccggcg ccgctcggtc
     5401 cgcaatgcag atatccttgc tcggccgtgc cgtcagtctt atcgcgaccc ggtgttcggg
     5461 tcgcggttcc gatcccggga gcaagggtgg cgaagattgg gttgaggcgc tggaccacat
     5521 ctggttcgcc gccaatcatc agacaatatc cgcgttcaga accccagacc ccgccgctgg
     5581 tcccaacatc gacatagtgg atactcttag gcttgagttc gctcgcgcgg cgaatgtcgt
     5641 ccacgtagta ggaattgccc ccgtcaatga tggtgtcgcc ggattgcatg cggccagcaa
     5701 gttcgtgcag agtctcatcg acgacagcgg caggcaccat cagccaagca gcacgcggag
     5761 gcttcaactt ggccacgaag ttatcgagcg aggcggctcc ggtcgcacct tcactcacta
     5821 acttttccac gctcgaggag ttcacatcga acaccacgca gtcgtgcccg ccgtgtcgca
     5881 agcgttgcac catgtttgag cccattcttc ccaggccaat cattcccagt tgcattttca
     5941 tcctcaattc acgtcaacaa cgcaccgcat ctcgtttgtt tcagattttc ttcggctgac
     6001 attctcgtct caggcaaatc aagcatgacc agcaaatcgc tcgtctttgc tgagtgtttc
     6061 aagcagcttt ttcatgtcgt cagcatgctc ttcctccatg gccagaatct tttccatcag
     6121 gatgcggctg gtggaatcgt cattaccgag gtaacggatg atctcgctat aggactcaat
     6181 cgcgatcctc tcggccacta ggtcctcccg gatcatgtcc agcagcgatt tgccttcgac
     6241 atactgtgaa tggctacgag ttgtcagacc atccggattg aggttgggtt cgccgttcaa
     6301 ttgcgtgatc cgctctgcaa tcatgtcggc gtgaccttgc tcctcatttg catgttggag
     6361 aaactcttcc gccacggcct gtgcatagcg taacgcttgt ctgtttccgc tggcagaata
     6421 aggaccggct tagtagccag cgaactcttc cgcgcggata tcgtcatcat ctactcccgc
     6481 cttgtttaac atctcgtgca agcctttcac catggcaggg ggcccggcaa taaagtagat
     6541 gggtgtcccg gcgcccttca gatgcctggt aagcatttct ggattgatca gccccgtttc
     6601 accatcccag ggccgttgtg atttcttcat ttccgtcatg ctggcgatca gcttgtagtt
     6661 tggatttgct cgctccaatg tttgaagctc ttcaaggaat ggagcatcct ctggccgacg
     6721 gtttgaatag aagaggaaaa tgcgatgtgg cagcttctcc ttcgccgccc gaaccagcat
     6781 acttcggaat ggcgtgatgc caatgccgcc cgcgaggagc gcgaccgctc gcttaaaatt
     6841 attggggaga gttaagtccc cggacgggcc ttcgatttta accgcagtgc cgattggcat
     6901 ggtcttcaac acacgcttga acgccgtatc acggatgcgg gtcgccacca tcagcatttc
     6961 ttcgtgagga ccgctcgcga tcgaaaaccc tcgcgtattt ccttctgaat ccgtctccga
     7021 tgggtcgagc aacgtcatgt cgataaattg acccgccttg aacgtccacc cggatggttt
     7081 ttcgaaccga aacgcgatgg ttcgctcggc gacttctttc cgatccttca gtttggacat
     7141 aaaaatcggc gatgccgcag ctgtgctcat cttttgtctc cctccgcaag cttctttcta
     7201 accctttaag gttcactcaa ctgctaccac gtaatctcgc ccttcccagc gattctcgac
     7261 cgtccagaaa aacgtaaagc gaatcggcgg ccgctgcgcg ttcgagatcg gaatatcgac
     7321 aatatggaat ccgagaactt gggccgatga gggactatct tccgcagact tccagtcgtt
     7381 ggccgaccag tgaaggctaa aggccgcagg gacctgaatg cgaagagtat aacctcgttt
     7441 gacgctgcga gcctgtcgat tgggtttcca gatctcgaac aattgacgag ccttgcgatc
     7501 tccgaggtag cgggttgcaa cttcaggaat tagatcgaaa acgcggctat cggatacgga
     7561 cctgagcagc ttgatgtatt ccgcgtgagc ccacataaga ggcatagccg agcccgtcgg
     7621 tcgaccaagg aacatataag cctccggccg gtcgggctcg tcccatactt gttcgggcaa
     7681 taagcccgtt gtcgatgcga atccttcgat tgctcgtatg aacggcttta catcatgtcc
     7741 aacggagagt tcatagtgag ctcgttctcc cgtcagaagc ggccatgccc ggcccttgcc
     7801 ccagccgatg taagggcctc cgtcctcccg ctggccgtaa ccgtcgtggt tgtaacgatg
     7861 ccataccggc ccgaccgaag tgtccacctt caataccgca tcaattacct tcaaagaatc
     7921 caggatcaac ggatcgtcgg ggcgcctgat cccgtagcgc acgagttgaa ggaagccggc
     7981 gtcgacgatg cctcttgcgg ggaatgaatc ctgtgctccg gctggatggt tcttaatctt
     8041 tagaactttt gagtttgggt cctccaaggg attggcattg tcgacttcct ccggcagaat
     8101 ccgtatgtag tgccgcttaa tccccgggac aagtgaaccc tccgttgtaa cggtccaact
     8161 ttcgatatga cattccagaa aatcggcgta atcctccaag agggttgcaa cagcctcatc
     8221 ccctcggtcg cgcgcaaagc atgccgcacc gatcagggca gcaatgttgg aagcgagcgt
     8281 ggaaggtgaa tagccactgg cttcttccca gcgctcctgc tgagtgacag gtccatggcg
     8341 cattagataa gcagccgctt tcaggaccat cgggtacggg tcaaaatctt gaagagcgtc
     8401 ctccctttgg agctgccagg cgagaagtat cggaaacgcg acttcgtcaa gctggattcc
     8461 ccgccagaat ggtccgccat caacccagaa attctgggca aagcctccgt cttgctgctg
     8521 tgaaactgca agataaatca aagcccgcag aggcgtgacg cggtcacccg cggcaagcat
     8581 cgccgaagcg ctgctgacca tatcgcgggt ccagaccaga tggtatccgc cgtggtccgc
     8641 atcacctttt gcttctcccc aaggaatcga gagagaggca atcaaggcgc ctggataaga
     8701 cttgtcctcg tgagcgagaa gtaagctgaa actgctgcgg tataggttgc ccttgtcgct
     8761 cgaacacttc tccaagggaa gcaggtgcga ggaagtgcgg tcccattgtt ccgtgtatcg
     8821 cttcaactgc tcattgaaag gaatgccgag cgcctgaaag aggtttgtga tcgcgcagtg
     8881 ctccgtgtca ccaaacgcca gccccatggt gaactcgcgg ttaccctcaa ggtccagttc
     8941 tccagtcagc gcaacgttcc cgtcaggtgc atcatcgaac tcccaatcca tttgaaagtt
     9001 actggcaagg tcggtccagc cgtcgctacg gccaacgtaa ccacacgaaa cacgagaaaa
     9061 ctggaccgtc ccgccgagtg ccatccatat tccttttttc ctggccatca ggatgttccg
     9121 gccagcagcc tcgattgcgt agccgttgtt accccatccg cccacttgaa gatgaggggc
     9181 acatagcgtg tacaggcgca gcttcgagat aaacgcatca tcgcccccaa ggtgaacgcg
     9241 ttgaagaatg catgctaggt gtggatccgt aataatttcc ttgatgacgg tgtaatgacc
     9301 gttgggatcg aggttcgtaa cgcggtagcc caaggcgtga tcggaaagac gctcacactt
     9361 cgacttgagg tcacgtcttt cttcatgaaa gaacgtcttt ccatcggtga tgagaaactg
     9421 caagtctcga acctgcgggt gatccacggt cggataatag acttcggtga tgacaccatt
     9481 ccacaaggtg aaccagattc ggctcgaagc tgcgtaggat gttcccactc cgtctttgtc
     9541 gccgtgagtc catcgtggct cgatgccagg tcgtccgaaa gcgctatctt tcttggtgga
     9601 tttcttgcga tcgccaacgg cgatggactg taacgtgttt cccgccttcg atcgggcgat
     9661 tctcgcaggt tgtgctttca tgatttaact ctatctcctt gttgcggtcc tatttcagcg
     9721 gtagtcgctt ctgcgaattc cggcgatacc ccgtcaccgc gcacgctacc aacggccttg
     9781 tgcctcgcgg aatggctcga gcaccatcat cgagctgacg agacggctct tcccttattg
     9841 cctgtcccct tcgctgccac atcggccact gtggctgtgg gatccgaaga atagctatcg
     9901 acatgcggac gaaggttcgt ctgcgcggga gcgttcttcg ccagagatgg ttttggcatc
     9961 actatttcga gcgtgccttc cttgaacgtc accttagctt tcgaaggatc gacctccacg
    10021 ggcagctcgg ccacatggaa aatctgctcc gcgggtagga gagaatgctc aagcgaatac
    10081 ataacggttc cgccagcgtt gcgatttccc gagagttccg ctctgccgcc gattctcagc
    10141 cagcgtgact cgaggctgac cttgagctcg tgcggactaa atccgggaac ctcggtgtgc
    10201 gcgatgagat ggtcgtcgga t
//
LOCUS       DATNDT010000119         4478 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2627320, whole genome shotgun sequence.
ACCESSION   DATNDT010000119 DATNDT010000000
VERSION     DATNDT010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627320"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..622)
                     /locus_tag="VN788_03755"
     CDS             complement(182..622)
                     /locus_tag="VN788_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19680.1"
                     /translation="MNIAPDTNATPPENPVPAPPAVADSSAQPVEIIPDQPAAPRKPG
                     SSVEEAASQPSAHPRAPQISPQLSPGDQAAYQRKTNDDVSVTEKNLEHTKGKQLSAPQ
                     RDLIEKIHSFISQSRDAGRDGDWSRAENLAQKARLLSAELINSL"
     gene            complement(764..2668)
                     /locus_tag="VN788_03760"
     CDS             complement(764..2668)
                     /locus_tag="VN788_03760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU19681.1"
                     /translation="MRLSLKTKFTLAISLLVLAVVAVVSALYIARLTRQTMHQANDTA
                     AFIAQQVYDACGNALKEAAERGETPASPGAADLREYVRRAFDNSSMLNSLIESDVGIS
                     PTIYEIAITDADGIALVSSDASLRDQIVAKRPPISSLVRAGFFRQMRELYGPPQTFQF
                     SLPFQVGTQPFGDIRVGLSSTLLRGQVLPGLVSAAYWGLGSVFVSTLLALLVSTIALA
                     PITRISAQLDRISAGQFDVEPVVERGDEFGIVSTKIVGIGKQLRDVREIFSALRENLD
                     QVMSGLEDGLLLFNAEGRAVLVSPSVEKFLGVRSSELRGSRLAEIFPAGHPFRNALSI
                     EGNRIEPTDSKEIMMENGARSQRIGVSVQVIREQGVQMGTLITLRDVESLERIGSQLQ
                     VSERLAALGRVTAGVAHEVKNPLNSMRLWLEILKANMPVEPEPQQAVKMLDSEIDRLD
                     RAVKTFLNFTKPVELNLQETDARDLVDEVLDAARPSIARASLMLSAERPSEPLLIAVD
                     RQIIHHAILNLVLNACDFTGPNGAIAVNLRRTGNLATIEVHDSGKGIPPEDQKKIFQL
                     FFTTRPGGTGIGLANTFRFIQLHNGQIEFESGPGRGTTFRVELPLVRVADESAPKLRD
                     FSQPFAAGNR"
     gene            complement(2646..4052)
                     /locus_tag="VN788_03765"
     CDS             complement(2646..4052)
                     /locus_tag="VN788_03765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU19682.1"
                     /translation="MASENASLQRILIVEDEDNARKGYEALLRKWDCEVLGVPSGEEA
                     LAKYAEFSPHVMIADVELPGMNGLELLRQLGDEILRVPVVIITGRGSDERVVQAIEAG
                     AFWYIEKPLKPPVLRALLDRALGRVRDQQAVAALRRDLRGAGKLGAMVGASNVMQELM
                     RQVEMAAPSTASVLITGETGSGKEIVARSIHMLSPRATRPFVAINCSAIPESLMESEI
                     FGHEKGSFTGAAERRIGCFELAHGGTLLLDEIGEMPAPTQAKLLRVLEDHKVRRLGSK
                     VETPVDVRVLAATNKNPEQAVASGHLRQDLYFRLNVFHIHLPPLREHKEDLQVLVDHL
                     LAEISAKHGKRVSGVGTDAMDLFRSYPWPGNVRELRNVLERAVIASDRGTIARQHLPP
                     DFGHAPAIGAPGLGGLRFPVGTTVDAAERELILQTLTATSQNKTRAAELLGISLKTLH
                     NKLKEYEAGRNNASQPEN"
     gene            complement(4150..>4478)
                     /locus_tag="VN788_03770"
     CDS             complement(4150..>4478)
                     /locus_tag="VN788_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19683.1"
                     /translation="VPGGRAPLVAIVDDVPGVQSVHVNFAREGDIEGNTQPRRTAYEA
                     AAAGALVGAAPGVAAHNKKDAAMGAAVGAVTAFMATSGLGEELTLEKGTKLEITLERS
                     LYLAEN"
BASE COUNT          910 a         1298 c         1326 g          944 t
ORIGIN      
        1 cacagcggga atgctcttct gcgagactgc gaaattctgg atcccttgca atgaggaact
       61 gccgcacgtc ttgggtggtg acgggcattt gttccctccg aggttggacc atcgctcaca
      121 tatcagtact acttaccata acccgggctg gtagccaata gggtgaattc cgtaccgcgt
      181 tttaaagcga attgatcaac tcggccgaaa gcaaccgcgc cttctgagcc aggttctcgg
      241 cacgagacca atcgccatct ctgccggcat ctcgcgattg agagatgaag ctgtgaattt
      301 tctcgatcaa atcgcgctga ggagcgctca attgcttgcc cttggtgtgt tccagatttt
      361 tttcagtcac ggagacatca tcatttgttt tacgttggta ggcggcttga tcgccgggcg
      421 ataactgcgg agaaatttgc ggcgcgcgtg gatgtgccga tggctgcgat gctgcctctt
      481 ccacggacga cccgggcttc cttggcgcgg cgggctggtc cgggatgatt tcaaccggtt
      541 gagctgacga atccgcgacg gctggagggg caggcacagg attctctggt ggagtcgcgt
      601 tggtatcagg ggcgatgttc atgggtcgct cggcggttgg cgggaccgga gccgctgcgg
      661 gactggccgc gcgaactcgc tgctgggaac aaccggcggt caagaaggaa agtccggcta
      721 ggaaggcaat gaagctaatg cgtgcgcttg gccgaataaa cattcatcga ttcccggctg
      781 cgaacggttg gctaaagtcc cgcaacttcg gcgcggattc gtcggcaacc cgtaccaaag
      841 gcagctccac gcggaatgtc gttccgcgac ctggtccgga ttcaaattcg atctgtccgt
      901 tgtgcagctg tatgaatcgg aaagtattcg ccaggccgat tccagtgccg cccggccgtg
      961 ttgtgaaaaa gagctggaag attttctttt ggtcctcagg agggattcct tttcccgagt
     1021 cgtgtacctc gatggtcgcg agattgccgg tgcgccgtag attgacggca atggcgccgt
     1081 tcggaccagt gaaatcgcag gcattcaaaa caaggttcaa gattgcatgg tgaataatct
     1141 ggcggtcaac cgctatgagc aagggctcgg agggccgttc agcgctgagc atgaggctgg
     1201 cgcgcgcgat cgaaggccga gcggcgtcga gaacttcatc gacaagatcg cgtgcatcgg
     1261 tttcctggag attcagctcc actggtttgg tgaagttcaa gaaggtcttc acggcgcgat
     1321 ccaggcggtc aatttcgcta tccaacatct ttactgcctg ttgaggttcg ggttcaaccg
     1381 gcatgttggc tttcagtatt tcgagccaca ggcgcatgga gttcagcgga tttttaactt
     1441 cgtgagccac gcccgccgtc actcggccca gcgcggcaag ccgctcggac acctgaagct
     1501 ggctgccaat ccgctcgagc gactcgacat cgcgcagggt gatcagcgtg cccatttgta
     1561 cgccttgctc gcgaataacc tgcacactca ctccgatgcg ttgcgagcgc gcgccgttct
     1621 ccatcattat ctctttgcta tcggtcggct cgatccggtt tccttcgata ctcaaggcgt
     1681 tgcgaaaggg atgccccgcc gggaaaattt cggccaagcg gctgcctcga agttcgcttg
     1741 agcgcacgcc caggaatttc tcaaccgacg ggctcaccaa cacggcgcgt ccctccgcgt
     1801 tgaagagcag tagaccgtct tcgaggccgc tcattacctg gtcgagattt tcgcgcagcg
     1861 cgctgaaaat ttcacgcaca tcgcgtaact gttttccgat tccgacaatc ttggtgctta
     1921 cgattccgaa ctcatcgccg cgctcaacga ccggttccac gtcaaattgg cccgccgaga
     1981 tgcggtcaag ctgcgccgag atgcgggtga tgggagccag tgcgatggtg ctgaccaaca
     2041 gagcgaggag cgtcgacaca aataccgacc caagtcccca ataggcggct gagaccagtc
     2101 ccggcaaaac ctgtccccga aggagggtag aggagagacc gactcgaatg tcgccaaacg
     2161 gctgcgtccc tacctgaaaa ggaagcgaga actggaacgt ttggggcggt ccatataatt
     2221 cgcgcatctg tcggaaaaag ccggcgcgca ccaaagaact gatcggcggc cgcttcgcca
     2281 ctatctggtc gcgaagggaa gcgtcgctgg atacaagtgc gatgccatcg gcgtcggtga
     2341 tcgcgatctc ataaatcgtg ggcgaaattc ccacatcgga ttcgatcaga gagttgagca
     2401 tactgctgtt gtcaaacgcg cggcggacgt actcacgtaa atcggctgcg ccgggcgacg
     2461 caggtgtctc gccgcgttcg gccgcttctt tgagggcatt gccgcaggcg tcgtaaacct
     2521 gttgcgcgat gaaggcagcg gtgtcatttg cctggtgcat ggtctggcgc gtaaggcggg
     2581 cgatgtagag cgcggatact actgcaacaa ccgccagcac cagcagcgag atcgccaggg
     2641 tgaatttagt tttcaggctg agacgcatta ttccgccccg cctcgtattc tttgagcttg
     2701 ttgtgaagag tcttgaggct aataccaagc agttccgcgg cccgcgtttt gttctggctg
     2761 gtggcggtca gggtctgcag aatcaattcg cgttcagcag catccacagt ggttccaacg
     2821 ggaaagcgaa gcccccccaa tccgggagcg ccgatggccg gagcatggcc gaagtccggc
     2881 ggaagatgct ggcgggcaat cgtgccccgg tccgaggcga tcaccgcccg ctccaacacg
     2941 ttgcgcaact cgcggacatt gccaggccaa ggataggaac gaaaaaggtc catggcatcc
     3001 gtgccgacgc cggagactcg cttgccgtgc tttgcggata tttcggcaag caagtgatcg
     3061 accaggacct gtaagtcttc cttgtgctca cgcagcggcg gtagatggat gtggaaaaca
     3121 ttgagacgaa aatataaatc ctgccgcaaa tgaccgctcg cgacagcctg ttcaggattc
     3181 ttgtttgtcg cggcgaggac gcggacgtcc acgggcgttt caactttgct gcctagacgc
     3241 cgaactttgt gatcctcgag gacgcggagc aatttcgctt gcgtgggggc gggcatttca
     3301 cctatttcat ccagcaacag agtgccgcca tgagccaact cgaagcaacc gatgcgtcgt
     3361 tcggccgcac ccgtgaacga tcccttctcg tggccgaaaa tttcgctctc catcagggat
     3421 tccgggattg ccgagcaatt gatggccacg aacgggcgcg tggcgcgcgg ggagagcatg
     3481 tggatcgagc gggccacaat ctctttcccg gatcccgttt ctcccgtaat caacacggat
     3541 gccgtggacg gcgcggccat ttctacctga cgcatcaatt cttgcatcac gttcgaggcg
     3601 cccaccatcg caccgagttt ccccgcgccg cggaggtcgc gccgcagggc ggccacggcc
     3661 tgctggtcgc gcacgcggcc gagagcgcgg tccagcagag cccgcaaaac cggaggcttc
     3721 aacggcttct cgatgtacca gaaggcacct gcttcgatag cctggaccac ccgctcgtca
     3781 ctgccgcggc cggtgatgat tactacgggc acccgcagaa tttcgtctcc cagctggcga
     3841 agcaattcga gtccattcat tcctgggagc tcgacatcgg caatcattac gtgaggactg
     3901 aactccgcat acttggcgag agcctcctcg ccgctgggca cacccagcac ctcgcagtcc
     3961 catttgcgta gtagggcttc gtagcccttg cgcgcgttat cttcgtcttc gacgatcaga
     4021 atccgctgga gagatgcgtt ctcggaagcc atctatgatc tgaatctacc agcagtttcc
     4081 agatattcca agggacggca acggaatcct gcgtcactgg agtccgaatg aggtcaacac
     4141 gcctgtgatt caattctcag ctagataaag cgaacgctcg agggtgatct cgagcttcgt
     4201 tcctttttcg agagtgagtt cttcgcccaa tccagaggta gccatgaatg cggtcacagc
     4261 gcccaccgcg gcgcccattg ccgcatcctt tttgttatgt gccgcaacac ccggtgccgc
     4321 gccaactaaa gcgcccgcag cggccgcctc atacgcggtc cggcgcggct gggtgttgcc
     4381 ttcaatgtcg ccctcccgag cgaaattcac gtgaacggat tgcactcccg gcacgtcgtc
     4441 cacaatagca acgaggggag cgcgtccacc tggcacgc
//
LOCUS       DATNDT010000120         2229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4119029, whole genome shotgun sequence.
ACCESSION   DATNDT010000120 DATNDT010000000
VERSION     DATNDT010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4119029"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..382)
                     /locus_tag="VN788_03775"
     CDS             complement(<1..382)
                     /locus_tag="VN788_03775"
                     /inference="COORDINATES: protein motif:HMM:NF013298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19684.1"
                     /translation="MPTGPPNSYRVAIVGASSLRGKEIKQVLEDRHFPASDVILLDEG
                     VMAGTLTEAAGEPTFIRALDEDSFEHANFVFFGGTVADAEKNWQTARKAGATVIDLTG
                     AVAADESSDSQTAWIPSLDSILPPR"
     gene            complement(386..1213)
                     /locus_tag="VN788_03780"
     CDS             complement(386..1213)
                     /locus_tag="VN788_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HXU19685.1"
                     /translation="MDFDSIDPIGREEEPGRQSRRKRLRRGIYILPSMFTVANLLCGY
                     YAILATLEGQPGYFDNAARAIGIAIVFDSLDGRIARAMGTNSEFGKEFDSLADIVSFG
                     IAPAFLAYAWGVHALGISNDAAPHLIPLGWLIGFFYVGCCAWRLARFNIQGMAPGGNR
                     FFAGMPTPAAAGMVAAVVHAFPTPVQDGRVSVLWLGLVVALGLLMSSTVRYYSFKDIP
                     WTRRQPSLAILLLVLLIGGIVLFSQITLLAIASAYFVHGIALYTVRSVRHRLASRHA"
     gene            complement(1195..1731)
                     /locus_tag="VN788_03785"
     CDS             complement(1195..1731)
                     /locus_tag="VN788_03785"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005010859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HXU19686.1"
                     /translation="MIIILLGGFIFYFFRDPERAIPPDPAAIVSPADGKVVDIVSEEL
                     DSRSGRRISIFLSIWNVHVQRAPVAGRIMEVTYCPGRFYGAFRARASAENEQNVIYID
                     TPQGILVFKQIAGAVARRVLCWKRKGEMVARGERVGLIRFGSRVDIWLPLEAEITVRR
                     GQMVKGGESILAKWTSIA"
     gene            complement(1949..2179)
                     /locus_tag="VN788_03790"
     CDS             complement(1949..2179)
                     /locus_tag="VN788_03790"
                     /inference="COORDINATES: protein motif:HMM:NF016231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF465 domain-containing protein"
                     /protein_id="HXU19687.1"
                     /translation="MPVTTQDVRQFLIARDPEFRSLAEEHSRCEFQLEQIVKSPYLSA
                     EDLVEEAMLKKLKLRLKDRMEFILSRHQEFAR"
BASE COUNT          522 a          615 c          658 g          434 t
ORIGIN      
        1 aacgtggcgg caggatcgag tcgagcgatg gaatccaagc ggtctggctg tcgctcgatt
       61 cgtcggcggc gacggcgccg gtcaagtcga ttaccgtggc gcccgctttt cgtgcggtct
      121 gccaattctt ttcggcgtca gctacagtgc cgccaaagaa tacgaagttt gcgtgttcga
      181 aactgtcctc atccagggcc cggatgaagg tgggttcgcc ggcggcttcg gtaagggtgc
      241 cggccattac gccctcatct agaaggatga cgtcactggc ggggaaatgg cggtcttcga
      301 gaacctgttt gatttccttg ccgcgcagcg aagatgcgcc gacaatcgct acccgataag
      361 agttcggtgg cccggtgggc atgggtcagg cgtgacgtga tgcgaggcgg tgcctcaccg
      421 accgcacggt ataaagggcg atcccatgga cgaaatatgc gctggcgatc gcaagaagcg
      481 tgatttggga gaaaaggacg atcccaccga tcagaagaac gagaaggagt atcgcgagag
      541 acggctggcg gcgcgtccac ggaatatcct taaagctgta atagcgcacg gtactggaca
      601 tcaagagacc gagcgcgacg actaggccga gccacaagac cgacacgcgt ccgtcttgaa
      661 cgggagtggg gaatgcgtgg acgaccgccg cgaccatacc tgctgcggcc ggagtcggca
      721 ttcctgcgaa gaagcgattg ccacctggcg ccatcccctg gatattgaag cgcgcgaggc
      781 gccatgcgca gcaaccaacg tagaaaaagc caattagcca gccaagcggg atcagatgcg
      841 gcgcggcgtc gttggaaatg ccaagtgcat ggacccccca cgcataggca agaaatgcgg
      901 gagctatgcc gaagctcacg atgtccgcga gcgaatcgaa ctcctttcca aactcactat
      961 tggtgcccat ggcgcgggcg atgcggccgt cgagggaatc gaacacgatt gcgatgccga
     1021 tcgcgcgagc ggcgttatcg aagtaacccg gctggccttc gagggtggcg agaatggcat
     1081 agtagccgca gagaagattg gccactgtga acatgctagg gaggatgtaa atgccgcggc
     1141 gcaatcgttt gcggcgcgat tgccggcccg gttcctcttc gcggccgatt ggatctatgc
     1201 tatcgaagtc cattttgcca gaatactctc accgcccttg accatttgtc cgcgccgaac
     1261 ggtgatttcc gcctccaggg gcagccagat atccacgcgg gatccaaagc gaatcaaacc
     1321 cacgcgctcg ccgcgggcga ccatttcgcc ttttcgcttc cagcatagca cgcgccgcgc
     1381 gacggctccg gcgatttgtt tgaagactaa tatgccttgt ggtgtatcaa tataaataac
     1441 attttgctcg ttttccgcgg aggcacgggc gcgaaaggcg ccataaaagc gtccggggca
     1501 ataggttact tccattattc gcccagctac cggcgcacgc tgtacatgca cgttccaaat
     1561 cgaaaggaaa atactgattc gcctgccgct acgggaatcg agttcctcgc tcacgatatc
     1621 cacgaccttg ccatcagccg gcgaaacaat tgcggcagga tcgggcggaa tcgcgcgctc
     1681 cggatcacgg aagaaataaa aaatgaaacc gccaaggaga attattatca cgccaggaat
     1741 ctcccagcca aaaaaaagac acccaacgcc gacaacgatg ggcgggaccg caaacctgta
     1801 cgcgtctcta atcatgaccg tatggatgga atctcgcgac gcagcaaaaa aacggccgat
     1861 tgcctgttgg caagacgcct gctgcggtga gaatagccct tttgaatgca gatgtattgt
     1921 acgacagaag cgaagcgctt accgggccct agcgggcgaa ctcttgatgc cgggagagga
     1981 tgaactccat ccgatctttg aggcggagct tcagcttttt cagcatggcc tcttcgacca
     2041 gatcttcggc actgagataa ggagatttaa caatctgttc tagctggaat tcacagcggg
     2101 aatgctcttc tgcgagactg cgaaattctg gatcccttgc aatgaggaac tgccgcacgt
     2161 cttgggtggt gacgggcatt tgttccctcc gaggttggac catcgctcac atatcagtac
     2221 tacttacca
//
LOCUS       DATNDT010000121         8708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_326157, whole genome shotgun sequence.
ACCESSION   DATNDT010000121 DATNDT010000000
VERSION     DATNDT010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8708
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326157"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..140
                     /locus_tag="VN788_03795"
     CDS             <1..140
                     /locus_tag="VN788_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXU19688.1"
                     /translation="FKSPMCAPELYPEHDLFIQLMKLKNTLRWMMGEELITHLGAEYY
                     D"
     gene            complement(214..537)
                     /locus_tag="VN788_03800"
     CDS             complement(214..537)
                     /locus_tag="VN788_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011062198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXU19689.1"
                     /translation="MAKRKIFDELMEGIEAMKGHRDGKITLRTYKVEAAPLPKVDSKL
                     IRDTRKRLRCSRAVFARRLRINERTLEKWEQGRARPNPQAAALVLLVRNFPDTLDRLE
                     RVAMG"
     gene            complement(543..911)
                     /locus_tag="VN788_03805"
     CDS             complement(543..911)
                     /locus_tag="VN788_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019365248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXU19690.1"
                     /translation="MEFFEAPAFTRFVSSYLTDDEYRAIQGRLSIAPEAGDVIPGTGG
                     FRKLRWAHAMRGKGRRGGLRVIYYYFSAANQIWFMTLYDKNEASDLTPKQKQALKGAV
                     EAETQTRQTSRLAKQGRARR"
     gene            1196..1826
                     /locus_tag="VN788_03810"
                     /pseudo
     CDS             1196..1826
                     /locus_tag="VN788_03810"
                     /inference="COORDINATES: protein motif:HMM:NF016207.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
     gene            2164..4380
                     /locus_tag="VN788_03815"
     CDS             2164..4380
                     /locus_tag="VN788_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU19691.1"
                     /translation="MFLRKWRVIKTGANDQATKTRANFRKIERREWWLWAGAFVITLV
                     LMGGLLSFFLPADRIFLNRYSPEVSPNIIRGLVGLVFLFDLYTIYQHLMIYRIRRQLL
                     HREELFHLISENAADMIAVVDMEGKRIFNSASYERVLGYTPEELEASSPYEQIHPDDR
                     DRLKQALDTARRTGTGQTLDYRFRHKNGTWLALESTSSVIRNPVGLPEKLVIVNRDIT
                     ERKRAEEALQRSEGDFRSVVEYAPYGIYRARADGRFLQANPALQKMLGYASNQEIFAK
                     NLKTEIFRHEAEYTRLTEILTRSEQIKDIEVEWKKQDGAPITVRCSGRRVNDAHGSLA
                     YFEVFAEDVTEKHALAKQLQMAQKMEAIGRLSGGIAHDFNNLLGVIIGYAGVLKKALG
                     EHGALQEHAVEIEKAGQRAASLTRQLLAFSRQQVLTPAVLDLNTLAADLEKMLPRLLG
                     EDIQISMELSHGLRKVKADRTQIEQVILNLAINARDAMPSGGHLRIATSNADLDQLYT
                     WNHAGSKVGSYVLLTVADTGIGMNSETLAHIFEPFFTTKEPGKGTGLGLATVYGIVKQ
                     SNGYIWVESAPGKGATFQVYLPQHAGEAQKNESRPDRGEPLSGSERILLVEDAEPLRK
                     LAQRFLEMGGFRVVSAGSGEEALQVAADHPGTFDLLLTDVVMPGMNGRALAERLLPRE
                     PGMKVLYMSGYTDSFIAGHGVLEKGLHLLHKPFTDDVLLRKVREVLDNVREPVGVR"
     gene            4382..5098
                     /locus_tag="VN788_03820"
     CDS             4382..5098
                     /locus_tag="VN788_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19692.1"
                     /translation="MSANTETTKTMDQESKSMGKLLVVDDDEALRRLIRIQLSDVYEI
                     VDSGEPDQGLALALESKPDAILLDLRMPKYSGYELLQTFSSFSRTHTIPVVIVSGEAG
                     GQTKEYCQQLGAAGYFEKPIDFDALRACLQDLIKSRPAVPQTEVRVRLRISLKLRGTD
                     ANGKQFEEPASTDEVSLSGFLCACNADMGTSSLVDVYLARHGAQHVGKARIIHVSANG
                     GPSRRYGCRFVEKTGPWVLQ"
     gene            5263..5544
                     /locus_tag="VN788_03825"
     CDS             5263..5544
                     /locus_tag="VN788_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU19693.1"
                     /translation="MTEKHAEPRHSEPTAREVEIIELLAKGKSNKQIALELGISVRTV
                     ESHRRHIMQKLRARSVVELVYYAMDRGIAAQPKSWSEDQAVMSIPEDLA"
     gene            5885..6349
                     /locus_tag="VN788_03830"
     CDS             5885..6349
                     /locus_tag="VN788_03830"
                     /inference="COORDINATES: protein motif:HMM:NF018925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HXU19694.1"
                     /translation="MKKITKLLIGAAGGVAGAALMGEAYKLTAKLVEAKPAKGEDATE
                     KVANSVATKFTGRKLRSPAKRTGGQIVHYAFGASMGMLYASLADAFPSATAGCGALFG
                     LSLYAGAHGLAVPALGLAPNPLENGAPRECAELSSHIAFGLTTETLRRLLTR"
     gene            complement(6406..7326)
                     /locus_tag="VN788_03835"
     CDS             complement(6406..7326)
                     /locus_tag="VN788_03835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HXU19695.1"
                     /translation="MSSRPSIFIAALACIAILLATLDSRLVQAQSADDWEKEAGGKMS
                     FDVASVKQDKGDVPGQSNIPLFSGNVFPPNGGRLSITNLPAFNFIVFAFKLSGSEGRT
                     LSRELPDWTLEERYDIEAHAPEGTTKDQMRLMMQSLLADRFKLAMHYETHQGSVYDLV
                     LVKPGKMGPHLRLYRSDEVPCSTPDAKDAQSTVEGEFPAICGGIQPLVASSPGMRRFG
                     ARNITMTSFANSLSLDRPIVDKTGINGTIDFVQEYYQVNTDHDQIQPDVSPGPTPTEA
                     MKDQLGLRLQPDTAPITRLVVDGIEKPTPN"
     gene            complement(7383..7877)
                     /locus_tag="VN788_03840"
     CDS             complement(7383..7877)
                     /locus_tag="VN788_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19696.1"
                     /translation="MKLVRVLSRLVVLLAAAGAFVGLTAMYYDSSPHGPDSRDWPTPT
                     DFAQWQAEIRHQPSAPEVGYFSEFAGTAIILAIFVWMGRMLFRLRLNPAPRNEGQLIL
                     LGLYETPQENPKPLATRATAVLALGILVITLSSHVATLRIGLVIVSSAAITLFLHAIR
                     RNEL"
     gene            complement(7874..8356)
                     /locus_tag="VN788_03845"
     CDS             complement(7874..8356)
                     /locus_tag="VN788_03845"
                     /inference="COORDINATES: protein motif:HMM:NF025716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HXU19697.1"
                     /translation="MKKHWRLRTTFWLDMALLVSVCVLQTVRFTGLVVHEWLGLAVIA
                     MVLAHLLFSWSWIVTQSRRLFAKQTIRERVNYLLNFALFLFVTAAIFSGILISQKAIP
                     TLTGTKASPDDMNLRWAVLHEEFATNVLLLAGLHVAVNWEWLLAATGNIFRRFRESAL
                     "
     gene            complement(8432..>8708)
                     /locus_tag="VN788_03850"
     CDS             complement(8432..>8708)
                     /locus_tag="VN788_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19698.1"
                     /translation="VVMFAYPREFRLRFQNEMLSTFSDLIHDEWERKGLSGIAHVWRS
                     ALAEVFSAAVPLRLQSPAVIAISVAILSSFALFAGLIGEVMHGFYGK"
BASE COUNT         2068 a         2513 c         2339 g         1788 t
ORIGIN      
        1 atttcaagag cccgatgtgc gcgcccgagc tttaccccga gcacgatctt ttcatccagc
       61 tcatgaaact caaaaacacg ctccgctgga tgatgggcga agaactcatc acccatctcg
      121 gcgccgaata ctacgactag caccggcgga aggcgattta tagagctcag gtttcgcgaa
      181 cccaacgaac gagcgcagca ggttcgcttc ccctcagccc atcgcaacgc gttcgagccg
      241 atccagtgtg tccgggaaat tacgaaccaa aagaacgagg gcggcggctt gaggattcgg
      301 cctagcgcgc ccttgctccc atttttcaag agttcgttcg ttgattcgca agcggcgcgc
      361 gaatacagcg cgtgagcagc gcaacctctt ccgcgtgtcg cgtatgagtt tcgaatcgac
      421 cttcgggagc ggtgcggctt caactttata ggtacgcagc gtgatctttc cgtcccgatg
      481 ccctttcatc gcctcaatgc cttccatcag ttcgtcgaaa atcttcctct tagccattga
      541 acctacctcc gcgcccttcc ttgtttcgcg agtctgcttg tctgccgcgt ctgagtctcc
      601 gcttcgacgg cgcccttcaa agcttgcttt tgcttgggcg taaggtccga tgcctcattt
      661 ttatcgtaaa gagtcatgaa ccaaatctga ttcgctgctg aaaagtaata gtagataacc
      721 ctcaggccac ctctgcgccc tttgccgcgc attgcatggg cccatcgcag cttccgaaaa
      781 cccccagtgc ccggaatcac gtcgccagcc tccggcgcta ttgacaatcg gccctgaatt
      841 gcccggtact catcgtcagt cagatagctt gatacgaagc gcgtaaaggc cggggcctca
      901 aaaaattcca ttcggctcct tctacgctgc tagcgtacca tttacaagtg tacgctattg
      961 gcgtatacag tcaatgctgg aaatccgcta ctattccaca aggcgcaaag gtatatggcg
     1021 taacgaggca tatgcgaact gggtggaatc cagtgtcgta ttacgcgcct gtccatgctt
     1081 ctcgatcagt gtcggtcaag cgccaaacga agactgcatc gcataatcat acctgtcttt
     1141 ctgaaaccaa atacttccct acggcccggc taaggttcgc gataatctac agcgtatgta
     1201 cctgccacct gcatttaaag aagaacgcct ggagacactc catgcgttga ttcgcgctca
     1261 tccgctggcc acgctgatca caacgggcgc cggtggactg ctcgccaacc ttgtgccgtt
     1321 cactttggta aatggcggag agaaaggcac actccgctgc cacgtcgcgc gagccaacga
     1381 tcaagtcgag gcgctgaaag cgggcgccga gacgttaatc gtgtttcaag gcccagaggc
     1441 gtatatcacc ccgtcttggt acgcatccaa aaaaggaaca cggacgtgtt gtgccgacgt
     1501 ggaattacgt tgtcgtgcaa gttcgcggca cgccccgcct gatggaagaa cccgcgtgga
     1561 taagagagca gatcagccac ctcacctcga tccacgaagg caagcgcacg aaaccgtgga
     1621 acgtaaccga cgcgccggaa ccgtacatcg agggccagat aaaagcgatt gtcggcgtcg
     1681 agattccgat cgcaatgatc gaaggcaagt ggaaagcaag ccagaaccgc ccagaggccg
     1741 atcgccacgg cgtggaagaa ggtctgcgcc aggaagcgtt agatgaaatg gccgcccttg
     1801 tagcacagaa gcgcaaaacc gactgagttc aggcgtcacc acaaaaacgg aatgtctttg
     1861 agaatgacat acttacgagg caaagcgaga agcgcctcgc cgtctgatgg attcactgta
     1921 cgtagtttcg cgatttagac ttcaggatag actcctcgtt tccgccccgc cgaaccattt
     1981 gtttttgttc cgaacaggga caaactaacg attacagcga agtcaaagtg gcctgccctc
     2041 gttccttttc gaacgcgagg caagcggtat tgcctggtgc ggcgggatat agaagtttcg
     2101 tgtcgattgc tgcggacaga ccggcgcggc acgcgacacg acaacccgga aggtatgaga
     2161 tcaatgtttt tacgcaaatg gcgtgttatc aagaccgggg caaatgacca agccacaaaa
     2221 acccgcgcaa atttccggaa aatcgagcgg cgcgaatggt ggttgtgggc aggcgcgttc
     2281 gttattacgc tggtcctcat gggagggctc ctgtcgtttt tcctccctgc agatcgtatt
     2341 tttctcaacc gctactcacc cgaagtatca ccgaacatca ttcgcggatt ggtagggctg
     2401 gtctttcttt ttgacttgta cacgatttat cagcacttaa tgatctaccg cattcgccgg
     2461 caattgctgc accgcgaaga gctatttcat ctcatcagcg aaaacgctgc tgacatgatc
     2521 gccgtcgtcg atatggaggg aaaacgaatc ttcaatagcg cctcctacga gagagtcctc
     2581 ggatacacac cggaggaact ggaggcctcg tctccgtatg agcagattca tcccgacgat
     2641 cgcgaccgcc taaaacaggc cctggatact gcacgccgca ccggcaccgg acagaccctc
     2701 gattaccgct ttcgccataa gaacggcact tggctcgcct tggaatcgac gtcaagcgtc
     2761 attcgcaacc cggtcggcct gcccgaaaaa ctcgtcatag tgaatcgaga tattaccgag
     2821 cgcaaaaggg cggaagaggc gctgcagcgg tcagaaggag actttcgctc cgtcgtcgaa
     2881 tatgcgcctt acggaattta tcgtgcccgc gccgacggcc gtttcctgca ggcaaatcca
     2941 gccttgcaga agatgctcgg atacgcctcg aaccaggaaa tcttcgccaa aaacctaaag
     3001 accgaaattt ttcgtcacga ggctgaatac acgcgcctga ccgaaatcct cactcgttcc
     3061 gaacagatca aggacattga agtcgagtgg aagaaacaag acggtgcccc gatcaccgtt
     3121 cgatgctctg gacgccgtgt gaacgatgcc cacggcagcc tcgcttactt tgaagttttc
     3181 gcggaagacg taacagaaaa gcacgcgcta gcaaaacaac tccagatggc ccaaaaaatg
     3241 gaggcgatcg gcaggctgtc cggtggaatc gcgcacgatt tcaacaatct gctcggcgtg
     3301 atcattggct acgccggcgt cttgaagaag gcgctgggtg aacacggcgc tctccaggaa
     3361 cacgccgtgg aaatcgaaaa agcgggtcaa cgcgcggctt ccttgaccag gcagttgctc
     3421 gccttcagcc gtcagcaagt tctcacgccc gcggttctcg acctcaacac cctcgcggcg
     3481 gatttggaaa aaatgcttcc gcgcctgctg ggcgaagata ttcagatatc catggagcta
     3541 tcgcatggcc tccgcaaggt gaaagccgat cgcacgcaaa tcgaacaggt gatcttgaac
     3601 ctcgccatca acgcgcgcga tgcgatgccc tccggaggcc acctcaggat cgcgacttca
     3661 aacgcagatt tggatcaact ctacacgtgg aatcatgccg gttcgaaggt cggcagttat
     3721 gtgctgctca ccgtcgctga caccggaatc ggcatgaact cggaaacgct cgcgcacatt
     3781 ttcgagccat tttttacgac gaaagaaccg ggaaaaggaa cggggcttgg tttggccacg
     3841 gtgtacggca tcgtgaaaca aagcaatggc tacatctggg tggaaagcgc tccgggaaaa
     3901 ggcgcgacgt tccaggtcta cctgccgcag cacgcaggcg aagcccagaa aaacgaatcg
     3961 aggcccgacc gcggcgaacc attgtccggt tctgagcgca tcctccttgt tgaagacgcc
     4021 gagcccttgc gaaagctggc acaacgattc ctcgaaatgg gcggattccg cgtcgtgtcg
     4081 gccggcagcg gcgaagaggc gctgcaagtg gcggcagacc atccaggcac gtttgacttg
     4141 ctgctgacgg acgtcgtgat gcccggcatg aacggccgcg ctttggccga gcgattattg
     4201 ccgcgcgagc cgggaatgaa agtgctctat atgtccggtt acaccgatag ttttatcgcc
     4261 ggccacggag tcttggagaa aggccttcat cttttgcata agccattcac cgatgacgtt
     4321 cttctccgca aagtccgtga agtgctcgat aacgtaagag agccagtggg agtgcgatag
     4381 cgtgagcgca aataccgaaa caaccaaaac catggatcag gagagcaaat cgatgggaaa
     4441 gctgctggtc gttgatgatg atgaagcgct gcggcggctc attcgcatcc agttgagcga
     4501 tgtttacgag attgtcgact ccggcgagcc ggatcaaggc ctcgcgctcg cgctcgagtc
     4561 caagccggac gccattctgc tcgatcttcg catgccaaaa tattcggggt acgaattgct
     4621 tcagacattt tcatctttca gtcgcactca tactattccc gtggtcatcg tcagcggaga
     4681 ggcgggtgga caaacgaagg agtattgtca gcagctcggt gccgccggct atttcgaaaa
     4741 gccgatcgat tttgacgcgc tgcgcgcttg tttacaggac ctcatcaaat cgcggcccgc
     4801 ggttcctcaa accgaagtcc gcgttcgttt gcgcatttct cttaagctgc gaggtacgga
     4861 cgcaaacgga aaacaattcg aagagcctgc gtccaccgac gaggtgagtc tcagtggatt
     4921 tctctgtgcc tgcaatgccg acatgggaac gagttcactc gtcgacgtat acctcgcccg
     4981 ccacggtgcc caacacgttg gcaaggcgcg gatcattcac gtgagcgcaa acggaggccc
     5041 atcccggcgc tatggttgcc gcttcgtcga gaagactggc ccttgggtgc ttcagtaacg
     5101 ctcgctagcc tgtttctttg ttcgcaagac taacaggctg cggaaattga ggggaattac
     5161 tgtgaaacgg ctgtcatatt tacggccaga atctgcttac tcttgtaagc gcgattttgt
     5221 tttcgtcttt tggaatattg aaacgcggga aaattctaac ttatgactga aaaacacgct
     5281 gagccccggc actcggaacc gactgcgcga gaagtggaaa taattgagct cttggcaaag
     5341 ggcaagtcga acaaacaaat agcactcgaa ctcggcattt ccgttaggac agtcgagtcg
     5401 caccgccgcc acattatgca gaaactccgc gctcgctcag tcgtcgaatt agtgtattac
     5461 gctatggacc gcggcatcgc agctcaaccc aagtcgtggt cagaagacca ggcagtcatg
     5521 agcattcccg aagatctggc ctagaccatg ccggcgccgc cggtgaggcg cagtcttcaa
     5581 tccaactcca acacgcgcat ccaccgccaa atttccgctc cggctaacgt ctcacaggct
     5641 cgagaagacg gcagccatct accctacagc gcgtcattta ttgacgcagt ccatgcgcaa
     5701 agctaaagct aaaagagcaa ggcggccctg tcccttcagg ggagtgactg cacacggtga
     5761 cccctgcgga actttgcagt ttttccgctt ggaagtcatc cttggccgcc ccgtcttttg
     5821 cggttttgcc gcttcgctct ggcacgcaat aaacagagtc cgcggcatca ccgacgagtc
     5881 ttccatgaaa aaaatcacga aacttctgat tggcgcagca ggtggagtcg caggcgcggc
     5941 gctgatgggc gaggcataca aactgacggc caagctcgtc gaggccaaac cggcaaaggg
     6001 cgaagacgca accgaaaaag tagcaaattc cgtcgcaacg aaattcaccg gcaggaaatt
     6061 gcgatcgccc gcgaagagga ccggcggcca aatcgtccac tacgcattcg gcgcatcgat
     6121 gggaatgtta tacgcctcgc tggccgatgc cttcccgtcc gctacagccg gatgtggcgc
     6181 gctcttcggc ctctcgctct acgccggcgc acacggcctc gcagtgccag ccctcggcct
     6241 cgcgcccaat cccctcgaaa atggcgcgcc ccgcgaatgc gccgaactct cctcccacat
     6301 cgccttcggg ctaaccaccg aaaccctccg ccgcctcctc acgcgctaac tatagtcgtc
     6361 gcgggaattt cttcgggaca agagggcgct acaacaacat tcggctcaat tcggcgtcgg
     6421 cttctcgatg ccatcgacta caaggcgtgt tatcggcgcc gtgtcgggct gcaacctcag
     6481 accgagttgg tctttcatgg cttcggtcgg agtggggccg ggcgacacgt ccggctgaat
     6541 ttgatcgtga tctgtgttga cctgatagta ttcttggacg aaatcgatag taccgttgat
     6601 tccagttttg tctacgatgg gacggtcgag agacaatgag ttcgcaaaag acgtcatcgt
     6661 gatatttcgc gcaccgaatc gacgcatccc aggtgagctt gcgacgagcg gctggatgcc
     6721 gccacagata gccgggaact caccctcgac tgttgactgg gcgtctttgg catcgggggt
     6781 cgaacacggt acctcatcgc tgcgatagag ccgcaggtgc ggccccatct tccccggctt
     6841 caccaaaacc agatcgtaca cagaaccctg atgcgtttca tagtgcatcg ccaacttgaa
     6901 acggtcggca agaagagatt gcatcatcaa tcgcatctgg tctttcgtgg tgccttccgg
     6961 ggcgtgagcc tcaatatcgt agcgctcctc aagagtccaa tctggaagct cgcgggacag
     7021 agtccttcct tcgcttccac tcagcttgaa agcgaagaca atgaagttaa acgctggaag
     7081 gtttgtgatt gacagacgcc caccgttggg tggaaatacg ttgccagaaa atagcggaat
     7141 gtttgactgc ccgggaacat cccctttgtc ttgcttcaca gacgccacgt cgaacgacat
     7201 tttgccgccc gcctcttttt cccagtcgtc ggcagactgt gcctggacta gccgactatc
     7261 aagcgtggcc aacaagatag caatgcaggc tagagctgcg ataaagattg atgggcggct
     7321 tgacatgagt cggttcctga ctggagggta cgccggagtg tgatggcgcc agcgatccat
     7381 tcctaaagct cgtttcttct aatcgcgtgc aagaacaggg tgatcgcagc ggaggacacg
     7441 atgaccaagc caatccgcag cgttgccacg tgcgacgaga gagtgataac gaggattcca
     7501 agggcgagga cggctgttgc gcgcgtggct aaaggcttag ggttctcctg cggtgtttcg
     7561 tacaagccca acaagattag ttgtccttcg ttgcgcggcg cagggttcag ccgcagtcgg
     7621 aatagcattc gtcccatcca gacaaaaatt gccaaaatga tggcagtacc agcgaactcc
     7681 gaaaaatatc cgacctccgg tgcagacggc tgatggcgta tctctgcttg ccactgcgcg
     7741 aaatctgtag gagttggcca atcgcgactg tcagggccat gcggcgaact gtcataatac
     7801 atcgcggtca gtccgacaaa cgcaccggcc gccgccaaga gaacgacaag ccgtgacagt
     7861 acgcggacca atttcatagc gcggactccc gaaaccgccg gaaaatgttt ccagtggccg
     7921 cgagcagcca ctcccagttg accgccacgt gaagccccgc caaaaggagc acgttcgtcg
     7981 cgaactcctc gtgaagcacg gcccagcgta agttcatgtc gtccggtgaa gccttcgtgc
     8041 cggttaacgt gggaatcgcc ttctgcgata tcaaaatgcc cgaaaagatc gccgccgtga
     8101 cgaaaagaaa aagagcaaag ttgagaaggt aattgacgcg ctcccgaatg gtttgctttg
     8161 cgaacaggcg gcggctctgc gtgacgatcc aactccacga aaacagtaaa tgcgcgagaa
     8221 ccatggctat cactgcgaga ccgagccact cgtggaccac aagcccagtg aaacgaaccg
     8281 tctgcaacac gcagaccgaa accagcaacg ccatgtccag ccagaaggtg gttctcaagc
     8341 gccagtgctt cttcaagcta aatctcctag ccttttcggc tttccccaat tgccgaccag
     8401 acttccgctc cgactcaacc tcaagccgct tctatttgcc gtaaaaccca tgcattactt
     8461 cgccaatcaa acccgcaaac aaggcaaaag aggaaaggat cgccaccgaa atcgcgatca
     8521 ctgcggggct ttgcaggcga agcgggacag ctgccgaaaa cacttccgca agagcggaac
     8581 gccacacgtg cgcgatcccc gacaggccct tccgttccca ctcgtcgtgg attagatcag
     8641 aaaacgtgct cagcatttcg ttctggaagc gcagccgaaa ttcgcgcggg tatgcgaaca
     8701 tcaccacc
//
LOCUS       DATNDT010000122         9479 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3061776, whole genome shotgun sequence.
ACCESSION   DATNDT010000122 DATNDT010000000
VERSION     DATNDT010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9479
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3061776"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(459..1730)
                     /locus_tag="VN788_03855"
     CDS             complement(459..1730)
                     /locus_tag="VN788_03855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19699.1"
                     /translation="MRRSWCVVAGLLMFLFGASNLNAQIDQFSYYGPRLQMDSLNNLS
                     IGCHVSAADYRFTPLHSGTLSTVTAYLMGGNSPVGYADDHKTDGTLDTTDPGGTLLLQ
                     VETDDGSNLHLPSGTVLGSFTLADTYTKIATSDDAFTPMAISPQPSLQSGNIYHLVFT
                     NTDTTEICEPGSNVGNYFSIDATYVFAPNQPQPTIPMNQFGALQIPEGGSQWVDLSDP
                     GTGNPDNQGNQGTTPLVSIQFADGTTGGLGYYQVSRAIVGSQSGTAQGVREDFMPTNS
                     ITVNSVSVRFAWIQGGELHVQFQDSTGGDLDDCYFEPGTDFPSQSGVAVWKTCAFSGP
                     YTMSGGSTYHVVMAVDTGTEYYAYGIENGAKTHDQPNGGTGTTYGSGTTYPNGLAEIK
                     PASSWLDFSPNGGSCNCFDWEMYFTSPASGG"
     gene            2049..2771
                     /locus_tag="VN788_03860"
     CDS             2049..2771
                     /locus_tag="VN788_03860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU19700.1"
                     /translation="MIALSSVRVYVQGLFTRMGARLEPGHIRFLNSTINYLEVGRWLK
                     FRGYASVPRVSTRTDLHLALAKRFAAERVLYLEFGVYRGASLRRWAAASPNAASEFHG
                     FDSFQGLPEDWDAVRRKGTFNMNGILPQFDDPRVVLHVGLFSETLPGFAIPPHNRLVM
                     NIDADLYSSADFVLRRLEPHIRLGTVLLFDEFCDRLNEMRAFEEYLQRTDARFRFLIG
                     TANLETVAFECTCVTRASGGGT"
     gene            complement(2911..5514)
                     /locus_tag="VN788_03865"
     CDS             complement(2911..5514)
                     /locus_tag="VN788_03865"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HXU19701.1"
                     /translation="MQLRAGAAIGVLLCCAAAAPAQQRRATTETKRPPAAHGDAHAAP
                     PRHDKPNDIVISRYYETVRFASDGSSTRTLQVRARVETAAGAQQLQELAFTLDQNTEK
                     LSVSYLRVLGADGSVESGTTGTVNEVTSAAIPGAPPYANYKLVRVPAPALKPGQAVEY
                     EVVRRVAKNPASNDFWYEHRFARDAIVRDERLEVDVPAGRAVRIESPGFAYETHAGKN
                     ETAYVWTHANLKIAEAAPAGTPESKKATTPDVRLTTFQNWPAVARWYADLELPATHGS
                     PELTAKALELTRGAADELGKVQAIYDYVARNTQHVFLPMGFGPSSSPSAVRALLGGDA
                     GFGFHPPRAAAEIFKDHFADFEDERVLLGAMLRAAGIASDAVLISPSRDPDNAAPSPS
                     QFDRVLTAVPLRGKLIWMDTRTTVAPFQLLASPLRGRDGLLVAVNGPGEIVRTPADPP
                     FPSTQRVEISAKVNSLGTLSGKIHYTLRGDTELLLRGEFHAAPREQWKEIGQNILNLD
                     GLDGDVTGVRPSDPLSTHEPFQLEMDFTQPAFLDWASARARVAAPLLTFGVPAAPRKE
                     SERAGPLIKLGSPLRIDARLRLTLPANLTVQPPAGVAVARDYAQFKSAYSVENGTLEA
                     ERSLDFTLRALPASRAGDYAAFTRAIQADEAQPLLIENARPGISSVPADATAGDLIEA
                     GAALLKAGNVRSAMPLLERATQLDPRHKQAWSYLGLGYLREKEPRRAIEAFRKQVAVN
                     PQDARAWDYLGITLEGQKQHADAAAAFRHAIELNPLDLVAYPSLGAILSEQGDYAGAV
                     GMFEKATIVLPENAQVRVGLAEAYLRADSREKAAASFEKACQLTRASAIRKEVASRMA
                     NAKLGVPKACL"
     gene            6109..6492
                     /gene="rpsL"
                     /locus_tag="VN788_03870"
     CDS             6109..6492
                     /gene="rpsL"
                     /locus_tag="VN788_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015129154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HXU19702.1"
                     /translation="MPTFAQLVRYGREKIRTKTKSPALEGCPEKRGVCVRVYTQTPKK
                     PNSALRKVARARLTNSAEVTTYIPGIGHNLQEHSIVLVRGGRVKDLPGVRYHVIRGTL
                     DAAGVENRKQGRSKYGAKRPKAATK"
     gene            6511..6981
                     /gene="rpsG"
                     /locus_tag="VN788_03875"
     CDS             6511..6981
                     /gene="rpsG"
                     /locus_tag="VN788_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HXU19703.1"
                     /translation="MPRKGWVLRRKIAGDPVYGSDLVQKFISSMMYDGKRSTAQTIFY
                     QAMDQVGKKANDDAFKLFKKAIENCKPVLEVKSRRVGGANYQVPVEVNPFRKQSLAIH
                     WLVLYARTRSGKSMTDKLAEEILDAANGRGGAVKKKEDVHRMAEANKAFAHYRW"
     gene            7010..9274
                     /gene="fusA"
                     /locus_tag="VN788_03880"
     CDS             7010..9274
                     /gene="fusA"
                     /locus_tag="VN788_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HXU19704.1"
                     /translation="MARQVQLEKTRNIGIMAHIDAGKTTCTERILFYTGVTYKIGEVH
                     EGTATMDWMEQERERGITITSAATTASWKRFNESYRINIIDTPGHVDFTVEVERSLRV
                     LDGAVAVFDAVAGVQPQSETVWRQADKYRVPRIAFINKMDRIGADFDHSIRSMRQRLS
                     AHPVPVTYPIGREDSFIGVIDFIEERAIIWKDETLGAEYEVLPLEKLWDAEYLKTRPA
                     LEAAVKAGALSAEFYKEHREKVVEYIAEHDDAILTKYLEQHKLEPAELRASLRRSTIA
                     LKLVPVVAGSAFKNKGIQPLLDAVIDYLPSPLDVPPVEGTTADGAEPELRRAADDQPF
                     AALAFKIMTDPYVGHLTYIRVYSGVLKTGHSVYNSTKKTRERIGRLLRMHANKREEIE
                     SIEAGDIAACVGLKNVTTGDTLCSEEKPIVLESIEFPAPVISVAIEPKTKADQEKMGT
                     ALGRLAQEDPTFRVHTDHDTGQTLISGMGELHLEIIVDRMMREFNVQATVGRPQVAYR
                     ETIVKPAEAEHRHIKQSGGRGQYGHVKLTVQPLPNAGHEDMHDMSTDELDELAKQVAG
                     KGGKWRFDKEHRLLFIDKVVGGVIPKEYIPPIEDGVKEAMENGVLAGYEMVDLAVVVT
                     DGTYHEVDSSEIAFKIAGSMAFKEACRRANPKLLEPIMRVEVVVPEEYMGPVISDLNS
                     RRGQLQGRESRAGSEIINVQVPLAEMFGYATDIRSRTQGRGSFTMHFSHYQQAPPNVA
                     EEVIGRTTGKAVRS"
     gene            9339..>9479
                     /locus_tag="VN788_03885"
     CDS             9339..>9479
                     /locus_tag="VN788_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXU19705.1"
                     /translation="MSKEKFERSKPHVNVGTIGHIDHGKTTLTAAITKVLSKHNPKVS
                     FRA"
BASE COUNT         1943 a         2866 c         2860 g         1810 t
ORIGIN      
        1 gaaaaagaaa agaaaatcag cgaagacgat aggaagcgct ccctcgaaga tctcgaaaag
       61 ctcacccagg ccgaatccaa aaaaatcgaa gacctctccg ccgccaagga aaaagaagtc
      121 ctcgaattta agtagccgcc gttccgtctg ctattgtcgt ccgcgcccca tcaaatggaa
      181 atgtacgggc gcagcacgct gcgcccggcc ttttcccgcg ctcaagccca tcgcacgccc
      241 cgccactctc atcgggccgc ggaagccacg ccgcactctg cggaacgcgc ctcctttgcc
      301 tgctccgcct gccgtgtctg ctgtacacgc ttttgctgca ccgctaccgc gagccgcgtc
      361 cccgcaatcg cgaacgcgcc agcgccgttg ctcccgatta tgatcgaacg cggcgtgatc
      421 cgccgaccac gccgcgctca gccaggtaca cagtgcgatt agccgccgct agctggcgag
      481 gtgaagtaca tctcccaatc gaagcaattg caactgccac cgttgggact gaagtccaac
      541 cagctgcttg ccggcttgat ctcggccaat ccattaggat aggtcgttcc gcttccgtaa
      601 gtcgtcccgg tcccaccatt gggctggtca tgtgttttgg cgccgttctc tatgccatat
      661 gcgtagtatt ccgtgcccgt atcgacggcc attacgacgt gatatgtgct tccgcccgac
      721 atggtgtacg gacccgagaa agcacacgtt ttccatacgg ccacgccgga ttgcgaaggg
      781 aagtccgtgc caggctcaaa gtagcagtcg tccagatcac cgccggtgga atcctggaac
      841 tgaacgtgaa gctctccgcc ctgaatccac gcgaaccgta cggacacgct attcacagtg
      901 atgctgttcg ttggcatgaa gtcctcccgc accccctggg cggttcccga ctgtgagccg
      961 acaatggctc ggcttacctg gtaatacccc agaccccccg tcgttccgtc agcaaactgg
     1021 atgctgacca aaggtgtggt gccttggttg ccttggttgt ccggattgcc agtgccaggg
     1081 tccgacaggt ctacccactg gctaccgcct tccggtattt gaagcgcgcc gaactggttc
     1141 atgggaatcg tcggttgtgg ttgattaggc gcaaatacgt atgtggcatc gatcgaaaaa
     1201 tagttcccga cgttcgaccc gggctcgcag atttccgttg tatccgtatt ggtgaacacc
     1261 aagtgataga tgttgcccga ttgcagcgac ggttgaggcg atatagccat cggcgtgaac
     1321 gcatcatccg aagtggcgat tttagtgtac gtgtccgcga gcgtaaatga gccgagcacg
     1381 gtcccggacg ggagatgcaa gttgctgccg tcatccgttt cgacttggag caacaacgtt
     1441 cctcccggat ccgtggtatc cagcgtgcca tcggttttgt gatcgtcggc atacccgact
     1501 ggcgagtttc cccccataag ataggcagtc acggtcgaca gcgtccctga atgtagcggc
     1561 gtgaagcggt aatccgcggc actgacatga catccaatgg aaagattatt cagtgaatcc
     1621 atctgtagcc tagggccata atatgagaac tggtcgattt gagcattgag gttggatgca
     1681 ccaaaaagaa acatcagcag ccctgcaact acacaccacg accttcgcat cgtagagctc
     1741 ctttaatctg tgattatgta ccccgccgta aatacgggct gtggcggaag gtacgctgca
     1801 caggacccat ctacaatcgg ggcctccgtg cattccttca cgatttttag aggcgaaggt
     1861 cggcgcgtag acctcaaaga tatataaggg tttggattaa gcagcttttg tcgcccaatg
     1921 ccttttttgt caccccaggc cccccggcgg gccctctaac ctacagtcac cacccgattg
     1981 cacaggcaag ggctccggta tttactgcaa ttatatttgc ggccaggttt gaagagagga
     2041 ggaggaaagt gatcgctctt tcgtcggtgc gcgtttacgt acaaggactg tttacgcgaa
     2101 tgggcgcgcg gcttgaaccg gggcatattc gctttttaaa ttcaacgatt aattatctgg
     2161 aagtcggccg gtggcttaag ttccgtgggt atgcgagcgt gccacgcgta tctacgcgga
     2221 ccgaccttca cctcgcgcta gccaagcgct ttgcagcaga aagggttctt tatttggagt
     2281 tcggcgttta tcgtggtgca tcgctgcggc gttgggcggc tgcctcaccg aacgcagcct
     2341 ccgagttcca cggtttcgat agtttccagg gattaccgga ggactgggac gcggttcgca
     2401 ggaagggaac cttcaatatg aatggtatcc tgccgcagtt tgacgatccc cgagttgtcc
     2461 tgcacgtggg tctgttcagc gaaacgttgc cgggatttgc aattccccct cacaatcgcc
     2521 tcgtcatgaa cattgatgcg gatctctact cgtcggctga cttcgtgctt cggaggctcg
     2581 agccgcacat ccgcctcggg acagtgctcc tctttgatga attctgcgac cgtttgaacg
     2641 aaatgcgggc atttgaggag tacctgcagc gaactgatgc ccgattccga tttctcattg
     2701 gaactgcgaa cctcgaaacg gtcgccttcg agtgcacgtg tgtcacccgc gcttctggtg
     2761 gaggaaccta ggcggcgaat cgttccaagc ccggttcacg cgaggggaat tccgcgcaaa
     2821 tcatgagtga taggaggggc ttctatcgct cacgtcaccc ccattttaca taccgcgctt
     2881 gactcttgct ctgagcgcgg ctggtccgcg ctacaaacag gccttcggca cccccagctt
     2941 cgcgtttgcc attcgcgaag ccacctcttt ccgtatggct gacgcgcggg taagctggca
     3001 ggctttctcg aagctggctg cggccttttc gcggctgtcg gcccgcaaat acgcctccgc
     3061 aagcccgacg cggacctgcg cgttctcggg cagcacaatc gtcgccttct cgaacattcc
     3121 caccgccccc gcgtagtcgc cctgctcgga caaaatcgcg cccagcgacg gatacgccac
     3181 caaatcgagc ggattcagtt caatcgcgtg ccgaaatgcc gccgcggcgt ccgcgtgctg
     3241 cttctggccc tcgagcgtga tccccagata atcccacgcg cgcgcatcct gcggattcac
     3301 cgccacctgc ttgcgaaatg cctcgatcgc gcgcctgggc tccttttcgc gcagatagcc
     3361 gaggcccaga taactccacg cctgcttatg ccgcggatcg agctgcgtcg cgcgttcgag
     3421 cagcggcatt gccgagcgca cgttcccggc cttcagcagc gcggccccgg cttcgatcaa
     3481 atccccggcc gtcgcatccg caggcacgct cgaaattccg ggtcgcgcgt tttcgatcag
     3541 cagcggctgc gcctcgtccg cctggatcgc ccgagtgaac gccgcgtagt cgcccgcacg
     3601 cgaagccggc aatgcccgca gcgtgaaatc aagtgaacgt tctgcctcga gcgtcccgtt
     3661 ctccaccgag tatgccgact tgaactgcgc gtaatcccgc gccacggcca ccccagcggg
     3721 cggctgaacc gtcaaattcg ccggcagcgt caaccgcagc cgcgcgtcaa tccgcagcgg
     3781 gctgcccagt ttgataagag gtccggcgcg ttcggattcc ttccgcggcg ccgcgggcac
     3841 gccaaacgtc agcagcggcg ccgccaccct cgcacgcgcg ctcgcccaat cgagaaacgc
     3901 gggctgcgtg aaatccatct cgagttgaaa aggctcgtgc gtcgaaagcg ggtcgcttgg
     3961 ccgcactcct gtcacgtcgc catccagccc gtcgagattc aggatgttct gtccaatctc
     4021 cttccactgt tcgcgcggcg ccgcgtgaaa ttccccgcgc agcagcaatt ccgtatcccc
     4081 gcgcaacgtg tagtggattt tgccggaaag cgtgcccaac gaattcacct tcgcggaaat
     4141 ttccacgcgc tgcgtcgaag gaaatggcgg gtccgccggc gtgcgcacga tttcgcccgg
     4201 cccgttgacc gccaccagca acccatccct gccgcgcagc ggtgaagcca gcaattgaaa
     4261 cggcgcaacc gtggtcctcg tatccatcca gatcagcttg ccgcgcaagg gaaccgccgt
     4321 aagtacgcgg tcgaattgcg aaggagacgg cgccgcatta tccggatcgc gcgaaggcga
     4381 gatcagcacc gcatccgaag caatgcccgc cgcgcgcagc atcgccccga gcagcacccg
     4441 ctcatcctcg aaatccgcga agtggtcctt gaaaatttca gccgcggctc gcggcggatg
     4501 aaagccgaag cctgcgtccc cgccaagcag agccctcacc gccgaaggcg aagagcttgg
     4561 accgaagccc atcggcaaaa agacgtgctg cgtattccgc gccacgtaat cgtagatcgc
     4621 ctgaaccttg ccgagttcgt ccgccgctcc gcgcgtcagt tcgagcgcct tcgcggtgag
     4681 ttccggcgag ccgtgcgtcg cggggagttc cagatcggcg taccagcgcg ccaccgccgg
     4741 ccaattttga aaagttgtaa gtcgcacgtc cggcgtcgtt gctttttttg attctggcgt
     4801 ccctgcgggc gccgcctccg cgattttcag gttcgcgtgc gtccacacgt acgcggtttc
     4861 gttcttgccg gcatgcgttt cgtacgcgaa ccccggcgat tcgatccgca ccgcgcgccc
     4921 ggcggggacg tccacttcca gccgctcgtc gcgcacaatc gcgtctcgcg caaacctgtg
     4981 ctcgtaccag aaatcattcg acgccggatt tttcgccacg cgccgcacaa cttcgtattc
     5041 gactgcttgg ccaggcttca acgcgggcgc cggaacgcga accagcttgt agttcgcata
     5101 gggaggtgca ccgggaatcg ccgcgctcgt cacctcattc acggtgccgg tcgtgccgct
     5161 ctcgacggag ccgtccgccc caagcacccg cagataactc acgctcaatt tttcggtgtt
     5221 ctgatccagc gtgaacgcaa gctcttgaag ctgctgcgcc cccgcggccg tttccacccg
     5281 cgcccgcact tgcaaggtgc gcgtgctcga cccgtcggac gcgaatcgga cagtctcgta
     5341 gtaccgggaa atcacgatgt cgttgggttt atcatggcgt ggcggcgcgg catgcgcgtc
     5401 gccatgcgca gccggaggac gctttgtttc cgtcgtcgcg cgccgctgct gtgccggcgc
     5461 ggcggccgcg cagcacaaca aaacgccaat cgccgccccc gcgcgcaact gcattcacgc
     5521 tcccccaatg gaaagagtaa aaaaactgga aatagactca ccagtgtagg tgtgccgccc
     5581 aaagccgtaa agcccgccgc ggcccgccgt gccgaaagcc aaaacaaatc ggcttctaat
     5641 ctacggtacg gggattagaa ttccgcgtaa ctgactgaaa acaaagggcc ggaacagttc
     5701 taatctacgg taaccgcact cccatcccgc atcccaattc aagattcccg cccgcctccg
     5761 aactcggttg acatgagttc ttgtttttcc tatacttgcg cggttgtgtt gaggccatgg
     5821 ggcctggtgt tgaaagggaa gtcttccttt ccccggaaca aagagcgacg gaacggggga
     5881 agtgaagagc ggccgaaatg gggcgccagc cccgcaaagc cgtgcgagcc tctccgcttt
     5941 tcgtagatgc agttcactcc tcggggtgaa gcgtcggcgc ggagtacctg ggtagttccc
     6001 tcggggtttg gcgacctttt ctgttaagca cccgcagagt cctgcgcggc gagtttgtcc
     6061 gcgcgccgat ttacaacgac attagtgtgg cgcatcggag ggaagagcgt gccgacgttt
     6121 gcacagctgg ttcgatacgg gcgggagaag attcggacga agacgaagtc gccggccctt
     6181 gagggctgcc cggaaaagcg cggcgtctgc gtccgcgtct atacacagac gcccaagaag
     6241 ccgaattcgg ccctccggaa ggtggctcgc gcgcgcctga ccaatagcgc cgaggtgacg
     6301 acgtacattc ccggcatcgg ccataacctg caggagcact cgatcgtgct ggtgcggggc
     6361 ggccgcgtga aggatctgcc gggcgtgcgc taccacgtta tccgcgggac gctcgatgcc
     6421 gcaggcgtcg aaaatcggaa gcaggggcgc tcgaagtacg gcgccaagcg gcccaaagcc
     6481 gccacgaagt aagcgcggga gaggaacgag atgcctcgca aaggctgggt attgcgccgg
     6541 aagattgccg gcgatccggt atacggcagc gatctggtgc agaagtttat ctcgagcatg
     6601 atgtacgacg gcaagcgctc gaccgcccag acgattttct atcaggcgat ggatcaggtg
     6661 gggaagaagg cgaacgatga cgcctttaag ctgttcaaga aggcgatcga gaactgcaag
     6721 ccggtgctcg aagtgaagag ccgccgcgtg ggcggcgcga actatcaggt tcctgtggaa
     6781 gtgaacccct tccggaagca atcgctcgcg attcactggc tggtgctcta tgccaggacg
     6841 cgctcgggca agtccatgac ggataagctc gcggaagaaa ttctggacgc cgcgaatgga
     6901 cggggcggcg cggtgaagaa gaaagaagac gtgcaccgca tggcggaggc taacaaggcc
     6961 ttcgcgcatt atcgctggta agggagaggc gcgaggcgcg gcaattttca tggccaggca
     7021 agttcaactc gaaaagaccc ggaacatcgg gatcatggct cacattgatg cggggaagac
     7081 gacctgcacg gagcggatcc ttttctacac gggtgtgacg tacaagattg gcgaggtcca
     7141 cgaaggtacg gcgacgatgg actggatgga gcaggagcgc gagcggggca taactatcac
     7201 ctccgcagct accactgcct cctggaagcg cttcaacgag agctatcgca tcaatatcat
     7261 tgatactccg ggccacgtcg atttcacggt ggaagtcgag cgttcgctgc gcgtcctgga
     7321 cggagcggta gcggtgttcg acgcggtcgc cggcgtccag ccgcagtcgg agacggtgtg
     7381 gcgccaggcg gacaagtatc gagttccgcg catcgccttc atcaataaaa tggaccgcat
     7441 cggcgccgat ttcgaccact ccatacgctc gatgcgccag cggctttcgg cgcaccctgt
     7501 tcccgtgacg tatcccatcg ggcgcgaaga cagctttatc ggcgtgattg atttcatcga
     7561 agagcgcgcg atcatctgga aggatgagac cctgggcgca gagtacgaag tgttgccgct
     7621 cgaaaagctt tgggatgcgg agtatctgaa gacgcgcccg gcgctggaag cggcggtaaa
     7681 ggccggggcg ctgagcgcgg agttctacaa agagcaccgc gagaaggtcg tcgaatatat
     7741 cgccgagcac gatgacgcga ttctgacgaa gtatctcgag cagcataagc ttgaacccgc
     7801 cgaattgcgc gcgtcgctgc gccgctcgac gatcgcgctg aagcttgtgc cggtcgtggc
     7861 cggctcggcc ttcaagaaca agggaattca accgctgctc gatgcggtga ttgattatct
     7921 tccgtcgccg ctggatgtgc cgccggtcga aggaacgacc gcggacgggg ccgaaccgga
     7981 gctgcgccgg gcggcggacg atcagccctt tgcggcgctg gcgtttaaga tcatgaccga
     8041 tccatacgtc ggacacctga cttatatccg ggtttattcc ggggtgctaa agaccgggca
     8101 ctcggtctac aactcgacca agaaaacgcg cgagcgcatt gggcgcctgc tgcgcatgca
     8161 cgcgaataag cgtgaagaga ttgaatccat cgaggcgggc gacatcgccg cgtgcgtcgg
     8221 attgaagaac gtgacgacgg gcgacacgct ctgtagcgaa gagaagccta tcgtcctcga
     8281 atccatcgag tttcccgcgc ccgtgatttc cgtagccatc gagcccaaga cgaaagccga
     8341 tcaggaaaag atgggaaccg cgcttggccg gctggcgcag gaagacccta ccttccgcgt
     8401 ccataccgac cacgatacgg gtcagacgct gatttccggc atgggcgaac tgcacttgga
     8461 aatcatcgtg gaccggatga tgcgcgaatt caacgtgcag gcaaccgtag ggcggccgca
     8521 ggtggcctat cgcgagacga tcgtgaagcc ggcggaagcc gagcatcggc acatcaagca
     8581 gagcggcggc cgtgggcagt acggccacgt gaagctgacg gtgcagccgc tgccgaacgc
     8641 cgggcacgag gacatgcatg acatgtccac cgacgaattg gacgaattgg ccaaacaggt
     8701 ggccggcaag ggcgggaaat ggcgcttcga taaggagcac cggctgctct tcattgataa
     8761 ggtggtcggc ggcgtgatcc cgaaggaata catcccgcct atcgaggacg gcgtaaaaga
     8821 agcgatggag aacggcgtcc tggcgggcta tgaaatggtg gatctcgccg tggtggtgac
     8881 cgacggcacc taccatgaag tggacagctc ggaaatagcg ttcaagatcg cgggttcgat
     8941 ggcgttcaag gaagcttgcc gccgcgcgaa cccgaagctg ctcgagccga tcatgcgcgt
     9001 ggaagtggtg gttccggaag aatacatggg cccggtgatc tcggacctga actcgcggcg
     9061 cgggcaattg cagggccggg aatcgcgcgc gggcagcgaa atcatcaacg tgcaggtgcc
     9121 gctggccgaa atgttcggct atgcaacgga tattcgcagc aggacgcagg gtcgcggcag
     9181 ctttacgatg cacttttcgc attaccagca ggcgccgccg aacgtggcgg aggaagtgat
     9241 cggccgaacg acaggcaagg ccgtccggtc gtaagtcgtc gcaagcgaag gattcgcccg
     9301 gcctggtccg ggcacgctga agcggaggaa gggcgcacat gtccaaggag aaattcgagc
     9361 ggagtaagcc gcacgtgaac gtggggacga ttgggcacat tgaccacggg aagacgactt
     9421 tgacggcggc gattacgaag gtgctgtcga agcacaaccc gaaggtatcg tttcgcgcg
//
LOCUS       DATNDT010000123        16033 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1155775, whole genome shotgun sequence.
ACCESSION   DATNDT010000123 DATNDT010000000
VERSION     DATNDT010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16033
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155775"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2045)
                     /gene="ligD"
                     /locus_tag="VN788_03890"
     CDS             complement(<1..2045)
                     /gene="ligD"
                     /locus_tag="VN788_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXU19706.1"
                     /translation="MKRALADYERKRNFAATPEPKGRAKKSAMPIFVVQRHAARRLHY
                     DFRLEVNGALASWAVPKGPPEERGEKRLAVHVEDHPIDYAKFEGEIPKGNYGAGQVQI
                     WDKGTYEIEGPEPAAAQIERGDFKFRLHGERLNGRFVLVKMQRSERGNEWLLIRKESD
                     AEPSVVQHAGKPPSISSRKPGEVTRKAILNPAELPGAKKASMPDQVQVALATLADKPF
                     SNPEWLFEIKWDGERALAFVRDGEVELRARSGRDITQEYPELKPLPKTLNARKAIVDG
                     EVVVLDEAGRSDFMRIQQRFGVLNPSVRLQQSAPVTYYAFDLLYYDGYDLRNVALESR
                     KELLRSLLTPSERVRFSDHQNEKGIELYEVAKQQGLEGIVAKRRDSIYPGKRSSFWLK
                     LKIVQDIDVVIGGWTAPRRSRERFGALLMGMYDADGKLEYIGSVGTGFTQNLLNSIYK
                     MLQELHTNDCPFRVRPKLKEVAHWVKPQLVARVKFGQWTHDKKLRAPVFLGFQEDRAA
                     SDCRFENQVTPGVAVPTGASVSDTSKSDPSTASGAAHTPREEKQRGTPPKMTPRERRG
                     KAGTQAAKERSKPSSTGEVAVGDLEKELASGRGESLAVDLDGKRVNLTHLNKIYFPED
                     RLRKRDVLLHYLRVAPFILPFLKDRPLVLKRYPNGIDEQFFFQKEATSAPDWIRTVQ"
     gene            2194..3030
                     /locus_tag="VN788_03895"
     CDS             2194..3030
                     /locus_tag="VN788_03895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HXU19707.1"
                     /translation="MASAVWKGTISFGLVSIPIRMYAAARAKRANLHQIHKVCNTRIK
                     QPLFCPHCDRIVDRSEIVKGYEYEKGQYVVIDPDEIKKITPSSGHTMEILTFLDGNEV
                     DPVYFESSYMALPESHAEKAYLLLLKALEDTNKIGIAKVTMHQREYTVFIRPRDHGLT
                     LHTMFYENEIASVEGYGKKYDIKLKPEEVKLANQLVESLSAPFKPQQYKDEFQARLSE
                     LIEAKLKGRAVIATPKAPHRAPVIDMMEALKKSLASGHGGKREPKVASVAPAHRARRK
                     AS"
     gene            complement(3547..4125)
                     /locus_tag="VN788_03900"
     CDS             complement(3547..4125)
                     /locus_tag="VN788_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19708.1"
                     /translation="MNLQHVNVKLMLDSPADADLAPLIPIFYSWIQGQVFEELLLDVA
                     DYRHVPAGPGVVLIGHQANYSVDNIDNRLGVRYNRKAELDGGNQDRLAQAARAALIAC
                     RRLEAEPAVAGKLQFNGRDLEIFINDRLLAPNSDSTFAEARPDLQRFGERLFRGSQYS
                     LTHDPDPRKLFAVSLKASDSFPLATLLHNLGD"
     gene            complement(4145..6400)
                     /locus_tag="VN788_03905"
     CDS             complement(4145..6400)
                     /locus_tag="VN788_03905"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HXU19709.1"
                     /translation="MKVTNASWKEELAGKMPEHLAREIDIFETEIALRKQGKIDERLF
                     AETRLRRGTYGQRYDNGERHDGTKVQQIPYPSGELTKGPNTMWDAPGMQRIKIPGGGM
                     NADQLETMADLAEEYSDGIAHVTTRQDFQLHYVHIEDTPSIMRRLAAANITTREACGN
                     SVRNVTACPYAGVCQDEVFDVTPYSRVMSKFLMGHPDTQNFGRKFKPAFSGCSQHACG
                     LTSLHDIGFIAATKNENGEEKRGFQVFVGGGLGAVPYMAKLFDSFVPVEEMLPLTQSV
                     SRVYARLGEKKNRSRARIKFLVQDLGIDKFRELVLEERKTLEPDPRWTDFVDGAERFE
                     EEPLRPGGELPASASPAFQRWLKHNTRIQKQAGYVTVTVALPLGDITADQLRSLADIV
                     RRFTKETVRTTVEQNFAIRWVSQSDLPAIYEALEAAGLGQYGAGAIVDVVTCPGTDTC
                     KLGISSSRGLAAELRKRLLEKGAEIDESVENLHIKISGCFNSCGQHHVADLGFYGVSR
                     KIAGYAVPHFQVVLGGEWEHNGGSYGLPVVAIPSKNIPEVVDRLTTRYLSDRTKGESF
                     KDFVKRVGKVEMKKLLEDLAKPPANPSDRSFFSDWGDPREYTLGDMATGECAGEVVFP
                     IEFDLAAAERQLFEAEVAFDGGRSEEAGKKAYLAMLQAARGLVKLEMPQVSDRPDEVV
                     SEFRARYYDTQKFWDPFAGGKFANYLFDAHRKSNDLHSEDSSRYLIDEAQLFIDAAHS
                     CYNKLGTPVSA"
     gene            complement(6668..8947)
                     /locus_tag="VN788_03910"
     CDS             complement(6668..8947)
                     /locus_tag="VN788_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXU19710.1"
                     /translation="MADTSSIVGSTISHYSVLEKLGGGGMGVVYEAKDTRLGRSVALK
                     FLPDDISQDAQSIERFRREARAASSLNHPNICTIYDIGGLEGRPFIAMELLEGQTLKR
                     RIAEKPIEISELLQIGIQIADGLEAAHAKGIVHRDIKPANIFLVQRGPAKILDFGLAK
                     LATTRRQIGNFPGESSTTQTTNLENEIFLTSPGSSVGTVAYMSPEQARGEEVDARSDL
                     FSLGVVLYEMCTGVVPFSGSTAALIFDGILHTAPAPVTKVNPQLPLALENIFAKALEK
                     DSDLRYQTAAELRTDLKRLRRDLGSSRHPAEEKTEGSHAHPSSIAPTTKSVAVLYFEN
                     LSGNKEDEYLRDGITEDVITELSKIRGLNTFSRPTVLAFRDKPVTPAQIGKQLGAAYA
                     LTGTLRRAGARLRINAQLVDTHTDFPLWSERFDREMKDVFEVQDEMARKIAEALRVTL
                     SPQELEALAVKPTENLQAYDLFLRGKRYARRQTRQDLEFALQMFENAVVLDPSFALAY
                     AACANACAMFYCNYSRDQLWVERARDASGKAVALRWDLPEVQVSQAWVLYAAELHNEA
                     VRMVKKAIERKRDCEGAFYMLCRALFAAGRYQEVVDIADTAIEASGEDYNVYVPIMNS
                     LGAMGKEEAKRNMTQRFTVALEVHLKQVPEDARARVLLGNYYAERGREDDALRELNLA
                     ITLRANEASILYNAACVYCSLKRKPEALDALRKAWEAGFQDAVWARRDPDIAILHGEP
                     EFERLYPDKSSTPSGSTPR"
     gene            complement(9002..10009)
                     /locus_tag="VN788_03915"
     CDS             complement(9002..10009)
                     /locus_tag="VN788_03915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19711.1"
                     /translation="MIAMKISRKLWTAIACAAALATASAAQQSPTQPRAQILSFTAGS
                     NSVQPGQSITLKWAVVNADRITLNRGVGIVAARGTRTVTPKVTTTYTLTALGFGGSGK
                     DTRSITVTVAGTTPAPAQSAAASEPPASRPVPRTPDGKPDLSGIYIAPFHALKPIGKI
                     TLKPGAEKYKVGPDYSFSLGEHCLPRGVPDTINEPYPIQIVQTPKQVVILYEAGELFR
                     VIPTDGRPHPKDLDPTWMGNSVGHWDGDTLVVDVIGVNDKVSVGEYRHTTAYHVIERF
                     ERPSYDTLKYSAIIEDPNVFAAPWTEASTLTLHPEWDIQEYICDENNHDYQKLFERYK
                     Q"
     gene            10124..10861
                     /locus_tag="VN788_03920"
     CDS             10124..10861
                     /locus_tag="VN788_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXU19712.1"
                     /translation="MADTTTTAPQLYESSGRNPVAPIWHTTVFVLLILGLSSLQATKL
                     PMLEAHGRTQIYLSGMAFEIALVLYVWLLGVRPQGKRLSDLVGGRWAKPMDVLRDIGV
                     ALLFWCAVIGFLLAMRLILGANAEGTRAVKMLLPRTTAEIISYLALCVTAGFCEELLF
                     RGYLQRQFFAWSGRVEMAVALQALVFGLGHLYQGWKGALTITLYGAMFGILAVMRKSL
                     RPGMIQHMMQDSFTGVVGSLLVRHGRL"
     gene            complement(10893..11696)
                     /locus_tag="VN788_03925"
     CDS             complement(10893..11696)
                     /locus_tag="VN788_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19713.1"
                     /translation="MSRKFSLRWPLLFSAFTFLSVLFPILAQAPAQSAGRASQRAPIS
                     MEQEHHHHLEFENSYVRVFFVEIPSHESTLFHRHDFPYLSVPPTGIGGAIPSSRGAAG
                     QGTGLPMIGYSPGNFSHAVSNSQDFALRNVAIELLRPQGGIHNRCEQAVRDQPKENCD
                     IAEPSAAAPATHTPLLETDELSVEAWHIAANVTAPPWNDRADILLASLGGVSVTSSGG
                     IDSVNVLKGGLLWLPAGSKPVFKTAPARDGHFIAITFKDSGSRPVSPSL"
     gene            complement(11702..12187)
                     /locus_tag="VN788_03930"
     CDS             complement(11702..12187)
                     /locus_tag="VN788_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HXU19714.1"
                     /translation="MELEVKIRGLMMDPVTNMPVVVLKEAQGAGILPIWVGVYEANAI
                     ALEIEKVQTPRPMTHDLLKNVLTGLNVHVQKVVVSDLRDDTFYALIWMERDGQTMSMD
                     SRPSDALALALRLDCPIFVEDEVLKNSKISSVVSEKSSNEELRKWLENLNDEDLGRYK
                     M"
     gene            complement(12249..13691)
                     /gene="miaB"
                     /locus_tag="VN788_03935"
     CDS             complement(12249..13691)
                     /gene="miaB"
                     /locus_tag="VN788_03935"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HXU19715.1"
                     /translation="MSDRQFDIALESLASREAMPSSAAVMRTLDSLDGPSPGGRASGA
                     AAGKRFFIETFGCQMNVHDSEKVAGVLLDRGYTAAPNPAEADLMLYNTCSIREKAAQK
                     VFSRLGEWRGVSKDKIIGVLGCLAQQEGEDIFERAPWVSLVCGSASYRKLPELIAEIE
                     SGGRRVMGLDLDTDEAFETDVTRRDNPFRAYLTIIEGCNYACSYCVVPHTRGPERSRA
                     SAAILSEVRRLADAGYTEIQLLGQTVNSYRDPSPRHLDFAGLLSEVAAIQGIRRVRFT
                     TSHPNDFCRAIVDAIDATPTLCDHIHLPVQSGSTRILQAMRRTYTREEYLEKISWIRA
                     ARRPVSITTDVIVGFPGESEKDFEDSISLLESVGYDGVYAFTYSPRPNTPASTMLDAV
                     PEAEKSRRLAVLQERQRQIQVQRNGELVGEVFEALVDGHHAARHQWAGRSTSNRVLNF
                     TSPRENLLGEYVQVHVTRAAPYSLFGEHLF"
     gene            complement(13770..14789)
                     /locus_tag="VN788_03940"
     CDS             complement(13770..14789)
                     /locus_tag="VN788_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008612 - peptidyl-lysine modification to
                     peptidyl-hypusine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase family protein"
                     /protein_id="HXU19716.1"
                     /translation="MITTKEKPTVSAEKHSANAISSFMKHHFRHFNAAVVIDAAEAYV
                     DHLSSGGKMLITLAGAMSTAELGLSLAEMIRRDKVHGICCTGANLEEDVYNLVAHDHY
                     RRVPQYRHLTPDQEVELLDQHLNRVTDTCIPEEEAMRRIERVVIEEWYAADRAGQRFF
                     PHEFLFKVLKAGKLKQYYQIDPKDSWMLAAAEKNLPMIVPGWEDSTLGNMFAAQVISG
                     KVKNATTVRGGVEYMIELARWYTETATATPLGFFQIGGGIAGDFPICVVPMLHQDLRR
                     ENVPLWAYFCQISDSTTSYGSYSGAVPNEKITWGKLSVDTPRFVIESDATIVAPLIFA
                     WVLGW"
     gene            15264..15902
                     /locus_tag="VN788_03945"
     CDS             15264..15902
                     /locus_tag="VN788_03945"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXU19717.1"
                     /translation="MARVILGLLLFLSIFSWALILQKSYQLGGIDRRTARFLQMFRSG
                     RGLSDPNTLRAGVGGTPLISVYAAGYRELEQQIGGPQSRVPASANKIRNANAVGVEMQ
                     VAAGEEVRRLEKGMSTLATIASVSPFIGLFGTVWGVMDAFSGLGDVGAASLRAVAPGI
                     SEALITTAAGLFAAIPALIAYNHYLHGIRAVASRMSDFAAEFVAKVETLYSS"
     gene            15909..>16033
                     /locus_tag="VN788_03950"
     CDS             15909..>16033
                     /locus_tag="VN788_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019568039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU19718.1"
                     /translation="MPQAQREVGTSLSDINMVPFIDVVLVLLIIFMITAPILQSG"
BASE COUNT         3387 a         4500 c         4639 g         3507 t
ORIGIN      
        1 atctggaccg tgcgaatcca atccggtgcc gaagtcgcct ctttctgaaa gaaaaactgc
       61 tcgtcaatcc cgttcggata tctcttcagc accagcggac gatcctttag aaatggcaaa
      121 atgaatggcg ccacgcgcag gtaatgcagc aaaacatcgc gtttgcgcag acggtcctcg
      181 ggaaagtaaa tcttattcag atgcgtcaga ttcacgcgct tgccgtccaa atcaaccgca
      241 agcgattccc cgcgcccgct tgcaagttct ttttcgagat cgcctacagc cacttccccg
      301 gtcgaacttg gtttcgagcg ctctttggcg gcctgcgtgc cggctttccc ccgccgctcg
      361 cgcggcgtca tcttaggcgg agtcccacgc tgtttttcct cgcgcggtgt gtgcgcagcg
      421 ccggacgcag tcgaaggatc gctcttcgat gtatcggaca ccgaagcacc ggtgggcaca
      481 gcgactcccg gagtcacttg attttcaaac cggcaatcgc tcgccgcgcg atcttcctgg
      541 aatcctagaa aaaccggggc gcgcagtttc ttgtcatgcg tccactgccc gaatttcacg
      601 cgtgcgacca gctgaggttt cacccaatgc gccacttcct tcagcttcgg ccggacgcgg
      661 aatgggcaat cattcgtgtg aagctcctgg agcattttgt aaatgctgtt cagcaagttt
      721 tgcgtgaaac ccgtaccaac gcttccgata tattcaagct tgccgtccgc gtcgtacata
      781 cccatcagca gcgcgccgaa acgctcgcgc gatctgcgcg gcgccgtcca gccaccgatc
      841 accacgtcaa tatcctgaac gattttcagt ttcagccaga aagatgaacg cttccctgga
      901 taaatgctat cgcgcctctt ggcgacgatc ccttcgaggc cctgctgttt ggcaacttcg
      961 tacagctcga ttcccttttc attttgatga tcggaaaaac ggacccgctc cgacggagtg
     1021 agcaagcttc gcagcaattc cttgcggctt tcgagtgcca catttcgcaa atcgtaaccg
     1081 tcgtagtaca aaagatcgaa cgcatagtac gttactggcg cgctttgctg cagccgcacc
     1141 gaaggattca aaacaccaaa gcgctgctga attcgcatga aatccgagcg cccggcttcg
     1201 tccaatacca cgacttcgcc atcaactatc gccttgcgtg cattgagtgt tttaggcagt
     1261 ggcttcagct cgggatattc ctgcgtgata tctctgccgg aacgggcgcg aagttccact
     1321 tctccgtcgc gcacaaaggc caaggccctt tcgccgtccc actttatttc aaagagccat
     1381 tcgggatttg agaagggttt gtccgcaaga gtggctagcg ccacctgcac ttgatccggc
     1441 atcgaagctt ttttcgcgcc cggcaattcg gccgggttca gaatcgcttt ccgtgtaacc
     1501 tcgcccggtt tgcggcttga aatggaaggc ggcttccccg cgtgctgtac caccgagggc
     1561 tccgcatccg attccttgcg aatcagcaac cattcatttc cccgttccga gcgctgcatt
     1621 ttcacgagga caaatcttcc gttgagcctt tcaccgtgca gtcgaaattt aaagtcgccc
     1681 cgctcgattt gtgcggccgc gggctccggt ccctcgatct cgtacgtgcc tttatcccaa
     1741 atttgaacct gcccagcgcc gtaattgcct ttcggaattt ctccttcgaa cttcgcatag
     1801 tcgatcgggt gatcctccac atgaaccgcc agccgttttt ctccccgctc ctcgggcgga
     1861 cctttcggga cagcccaaga cgccagcgcc ccattcactt ccaagcggaa gtcgtaatgc
     1921 aaccgccgag ccgcgtggcg ctgcaccaca aaaatcggca tggccgattt cttagcgcgc
     1981 cccttcggct caggagtcgc cgcaaaattc cgtttccgct cgtaatcggc cagcgcgcgc
     2041 ttcacgaaat tagtataaac ggtgtcgact tgcttttaag ctgctgacgt gaaaaaaggc
     2101 gcggaaatgg cgcattaggc cgcgttgccc ctaaggtctt tcctttattg cgcgatttca
     2161 gcggggcgct atgctcgttg gcggagggtg tctatggcct cagccgtatg gaaagggacc
     2221 atttcgtttg gcctagtttc gattccgatt cgcatgtacg ctgcagctcg cgcgaagcgc
     2281 gccaaccttc atcagatcca taaggtgtgc aatacacgaa tcaagcagcc gcttttctgt
     2341 ccgcactgcg accgcatcgt cgatcgttcc gaaattgtca aaggctacga atatgagaag
     2401 ggccagtacg tcgtcattga tccggatgaa atcaagaaga tcacgccttc atcgggtcac
     2461 acgatggaaa tcctcacttt ccttgatgga aatgaagtcg atccagtcta tttcgaatct
     2521 tcctacatgg cattgccaga atcacacgcc gaaaaagcgt atctgctcct gctgaaggct
     2581 ctcgaagaca ccaacaagat cgggatcgcc aaagtgacca tgcaccaaag ggaatacacg
     2641 gtcttcattc gaccgcgcga ccatgggctg accctgcata caatgttcta cgagaatgaa
     2701 atcgccagcg tggaaggcta cggcaaaaaa tacgacatta aactcaagcc tgaggaggtc
     2761 aagctggcca accagcttgt cgaaagcctt tcggcgccgt tcaagcctca gcaatataaa
     2821 gacgaatttc aggcgcgtct cagcgagttg attgaagcca aactcaaggg ccgcgcggta
     2881 atagccacgc cgaaggcgcc gcatcgcgcc ccggtaattg acatgatgga agctctgaag
     2941 aaaagcctcg cctccggaca tggcggcaag cgcgaaccta aagtcgcatc ggttgcgccc
     3001 gcgcatcgcg ctcgccgaaa ggcaagttag ccttcttcat ccgcaccgag cggatgtcta
     3061 atttatcttc gccccataat ttatcggtaa ttgccccctc gcagtacagc gtatccctga
     3121 tggaaagaac ccctttggat ttccatactg gattcgggaa cacagttctc acagcaacgc
     3181 gaaaggagtt actgatggca ttgcgcaata ttcgaacgaa ccagccgagc caagggccag
     3241 gcctcacccc gattgacaac gcagcgcgaa gcggacgcga gcttgaaaaa tacgagcccg
     3301 accgaaccac gggacgtttg cccgcccaag aaaagaccag gaaaccaaaa agtaagaagc
     3361 gggcgcggcg taatggctag ccgagagcgg gagcgcccgc gcgtgcggaa accggacgct
     3421 cacgctcgcc aatcatatcc cccgcgacgc ttcgacaaat atgcctggat caatcatgtg
     3481 ctgggctaca aacggagatt cgcgtggctg gccgcgaaat gactaaataa tttggccgac
     3541 agaaaactaa tcgcccaaat tgtgaagcaa agttgccaat ggaaaagaat ccgaagcttt
     3601 taacgaaact gcgaatagtt tccggggatc tggatcatgc gttagagagt attgcgagcc
     3661 gcgaaacagc cgctccccaa atcgctgaag atctggacga gcttctgcaa aggttgagtc
     3721 ggaattcggc gccagcagac gatcattgat gaaaatttca aggtcgcggc cattaaactg
     3781 aagcttcccc gcaacagcgg gctcagcctc caaacgccgg caagcgatca atgccgcgcg
     3841 cgcagcctgc gccaaacgat cctgattccc gccatccaat tccgctttcc gattgtatcg
     3901 aacgcctaga cggttatcga tattgtccac gctgtagttg gcctgatggc cgatgaggac
     3961 gacgccaggg ccggcgggta cgtgccggta gtcggccacg tccaggagca gttcttcaaa
     4021 aacttgacct tgtatccagg aatagaaaat cgggatgagc ggcgcgaggt ccgcgtcggc
     4081 cggcgagtcc agcatcagct tcacattcac atgctgaaga ttcacgaggc tggaagttcc
     4141 ttgctcaggc gcttaccggc gtgcccaatt tattgtagca actgtgcgcg gcgtcgatga
     4201 atagttgcgc ttcatcgatc aggtagcggc tggaatcctc gctgtgaaga tcgttcgact
     4261 tgcgatgagc gtcgaagaga taatttgcga actttccgcc cgcaaaggga tcccagaatt
     4321 tctgcgtgtc gtaatagcgc gcgcgaaatt cactgaccac ttcgtccggc ctgtccgaga
     4381 cctgcggcat ttcaagttta accaggccgc gcgcagcctg gagcatcgcg aggtaagctt
     4441 tcttaccggc ctcttcgctg cggccgccat cgaaggcgac ctccgcctca aatagctggc
     4501 gttcggcggc ggcaagatcg aactcgatcg ggaacaccac ttcgccggcg cattcgcccg
     4561 ttgccatgtc gcccagagtg tattcgcgag gatcgcccca gtcgctgaag aaggaccggt
     4621 cggagggatt ggcgggaggt tttgccaaat cctccagcag cttcttcatt tcgacttttc
     4681 ccacgcgctt gacgaaatct ttgaaacttt cgcccttagt ccggtcggac aagtagcgcg
     4741 tcgtgagacg gtccacgact tcaggaatat tcttcgatgg gatcgccaca actggcaaac
     4801 cataggagcc gccgttgtgt tcccattcgc ctccgagtac tacctggaag tgaggcacag
     4861 catagcccgc gattttgcga ctgacgccat aaaagcccaa atcggccacg tgatgttgcc
     4921 cgcagctatt gaagcagccg ctgattttga tgtgcagatt ttcgacggac tcatcgatct
     4981 ctgcgccttt ttcgagcagg cgcttgcgga gttccgcggc aagtccgcgc gaagacgaaa
     5041 ttcccagctt gcacgtatcg gtgccggggc aagtgactac gtcgacgatg gcgcctgcgc
     5101 cgtattggcc gagaccggct gcttccaggg cttcgtaaat cgccggcaaa tcgctttggc
     5161 tcacccaacg gatggcaaaa ttctgttcga cggtagttcg aaccgtttct ttcgtgaagc
     5221 ggcgaacaat gtcagccagg gagcgcaact gatcagcggt gatatcgccg agaggaagcg
     5281 ccacggtcac agtgacgtaa ccggcctgct tctgaatccg cgtgttgtgt ttcagccagc
     5341 gttggaaggc cggcgaagcg gatgcgggca attcgccgcc cggccgcaaa ggctcttctt
     5401 cgaagcgctc ggcgccatcc acaaagtcgg tccagcgcgg atcgggttca agagtcttcc
     5461 gctcttcgag gaccaattct cggaatttgt cgatacccaa atcctgaacc aagaacttga
     5521 ttcttgcgcg gctgcgattt ttcttttcgc caagcctggc ataaaccctg gaaaccgact
     5581 gagtgagcgg caacatttct tcgacgggaa caaaagaatc gaaaagctta gccatgtacg
     5641 gaaccgcgcc gagcccgccg ccgacaaata cctggaagcc gcgcttctct tccccgtttt
     5701 catttttggt ggcggcgatg aaaccaatat cgtgcaagct ggtgagtccg caggcgtgct
     5761 gcgagcagcc gctgaatgcg ggcttgaatt ttctgccgaa attctgggta tcaggatggc
     5821 ccatcaaaaa ttttgacatc acccgcgaat agggagttac gtcgaacact tcgtcctggc
     5881 agacaccggc ataggggcag gccgtgacgt tacgaaccga atttccgcaa gcctcgcgcg
     5941 tagtaatgtt cgccgccgcg agccggcgca ttattgacgg cgtgtcttcg atgtgaacgt
     6001 agtgaagttg aaaatcctgg cgcgtggtga cgtgcgcgat gccatcggaa tattcctcag
     6061 ccagatcggc catcgtttcg agctgatcgg cgttcatgcc gccgcctgga atcttgatgc
     6121 gctgcatgcc gggcgcgtcc cacatggtgt tggggccttt ggtcagctcg ccggatggat
     6181 acggaatttg ttgaaccttg gtgccgtcgt ggcgttcgcc gttatcgtaa cgctggccgt
     6241 aggtgccgcg tcgcaaacgg gtttcggcga aaaggcgctc gtctattttg ccctgtttcc
     6301 ggagggcgat ttcggtttcg aaaatatcga tctcgcgagc cagatgctcg ggcatcttgc
     6361 cagcaagctc ttctttccaa gaagcgttgg ttactttcat aaaatcaatc agatgctgcc
     6421 tgtatggagt cgatgcatca aggatagcaa atccgcggtg gaagtgcatc cttgggcgac
     6481 cgcgcggcgg gcaaaggaag cgcgatgcag acagatggct tgaatctggg gtcctcaaaa
     6541 agtgtcgagt agaacgtacc gtcgcccttt cttttctgta agttgcgcca tgttctattc
     6601 ggcctgcctc gaagattggt gccgcaattt tgctgaaccc gcgaattcgt ggttgtcatc
     6661 cagttcgcta gcgaggggtg gagccggagg gcgtggagga cttgtcgggg tagaggcgct
     6721 cgaattcggg ctcgccgtga aggatggcga tatcggggtc gcgacgcgcc cagacggcat
     6781 cttgaaagcc ggcttcccag gctttgcgca gcgcatccag cgcttccggc ttccttttga
     6841 gcgagcagta aacgcacgcg gcattgtaga gaatcgaggc ttcgttggca cgcaaggtga
     6901 tcgccagatt gagttcgcgc agggcatcat cttcccggcc tcgctcagcg taatagttgc
     6961 ccagcaggac gcgggcgcgc gcatcctcgg gcacctgctt taggtggacc tccagcgcga
     7021 cagtgaaacg ctgggtcata tttcgcttgg cttcttcttt gcccatcgca cccagtgaat
     7081 tcatgatcgg gacataaacg ttgtagtcct caccgctcgc ctcgatcgcg gtatccgcaa
     7141 tatccacaac ctcctggtag cggcccgcag cgaagagcgc gcggcaaagc atgtaaaagg
     7201 cgccttcgca atcgcgcttg cgctcgatgg cctttttcac catgcgcacg gcttcgttgt
     7261 ggagttcggc agcgtagagg acccaggctt ggctgacttg aacctcgggc aaatcccagc
     7321 ggagggcgac ggcttttccg gacgcgtcgc gcgcgcgttc gacccacaac tggtcgcggc
     7381 tgtaattgca gtagaacatg gcgcaggcat tggcgcaggc ggcataggct aaggcgaagc
     7441 tggggtccag cacaaccgcg ttctcgaaca tttgcagcgc aaattcgagg tcctggcgag
     7501 tctggcgtcg cgcgtaacgc ttgccgcgca aaaagagatc gtaggcttgc aggttttccg
     7561 tgggctttac ggccaaggct tccaattctt gcggtgaaag ggtgacgcgc agggcttcgg
     7621 cgatcttgcg agccatttcg tcttggactt cgaaaacgtc tttcatttcg cggtcgaagc
     7681 gctcggacca cagggggaaa tcggtgtgcg tatcgacgag ttgggcgttg atgcgcaggc
     7741 gagcgccagc acgccgcagc gtgccggtaa gcgcataggc cgcgccgagc tgcttaccaa
     7801 tctgcgccgg agtaacgggc ttatcccgaa aggctaaaac ggtgggacgc gaaaaggtgt
     7861 tcagtccacg aatcttggaa agttcggtga tcacgtcttc ggtgatgcca tcgcgcagat
     7921 actcgtcttc cttgttgccg ctgagattct cgaaatagag tacagcaacc gatttcgtgg
     7981 tcggcgcgat gcttgaagga tgcgcatgcg aaccttcagt tttttcctcc gcaggatggc
     8041 ggcttgaacc cagatctcgc cgcagccgct tcaggtctgt gcgaagctcg gcggctgtct
     8101 ggtaacggag atccgaatcc ttttcgagcg ccttggcgaa aatgttttcc agcgctaaag
     8161 gcaattgagg attcaccttg gtcacgggag cgggcgcggt gtgcaggatg ccgtcgaaaa
     8221 ttaaggccgc tgttgagccg gaaaaaggca ccacgccagt gcacatctca tagaggacca
     8281 ctccgagcga aaacaaatcc gagcgtgcgt ccacttcttc gccccgcgcc tgttccggag
     8341 acatgtaggc cacagtgccc acggaactgc cagggctggt gagaaatatt tcgttctcaa
     8401 gattcgtcgt ctgcgtagtg gatgattccc ctgggaaatt tccgatctgc cgccgcgtgg
     8461 tggcgagctt cgcgagaccg aagtccagta tcttcgccgg acctctttgg accaggaaaa
     8521 tattcgcggg cttgatatcg cggtggacga tgcccttggc gtgcgcggct tccaagccgt
     8581 cggcgatctg aatgccaatc tgcaaaagtt cggaaatttc gatgggcttt tccgcgatgc
     8641 ggcgcttgag ggtctggccc tcgagaagtt ccatggcgat gaacggccgg ccctcgagtc
     8701 ctccaatatc gtaaatggtg cagatattcg gatgattcaa agaggatgcg gcgcgcgcct
     8761 cgcggcggaa tcgctcgatc gactgcgcgt cctgggaaat gtcgtcggga agaaatttta
     8821 gggccacgct gcggccgagg cgagtgtcct tggcttcgta gaccacaccc ataccgccgc
     8881 cgcccaactt ttcgagaacg ctatagtggg agatggtgct gccgacaatc gaagaagtgt
     8941 ccgccatcgg gcatgcagtt taccctagcg cctcagcgca ggcaaacgcg ccgcgattcg
     9001 attattgctt gtagcgctcg aatagtttct ggtagtcgtg attgttttcg tcgcagatgt
     9061 actcttgaat gtcccattcg ggatgaaggg tgagcgtgct cgcttcggtc cacggcgcgg
     9121 cgaacacgtt gggatcttca atgatggctg agtatttcag cgtgtcgtat gacgggcgct
     9181 cgaagcgctc aatcacatgg tatgccgtgg tatggcgata ctcgccaaca gagactttgt
     9241 cgttcacgcc gataacatcg accactagcg tgtcgccgtc ccaatgcccg acggaatttc
     9301 ccatccatgt aggatcgagg tcttttgggt gcggacgccc gtccgtgggg attacgcgga
     9361 aaagctcgcc cgcttcgtag aggatcacaa cctgcttcgg cgtttgcacg atttgtatgg
     9421 gatacggctc gttgatcgta tccggcactc cgcgcggcag gcaatgctca cccagcgaaa
     9481 aagaatagtc gggaccgact ttatatttct ccgccccggg cttaagcgtg attttgccga
     9541 tcggcttgag cgcgtgaaag ggcgcgatat agattcccga gaggtcgggc ttgccgtcgg
     9601 gtgtgcgcgg cactggccgg ctcgcgggtg gctcgctggc tgcggcactc tgggcgggcg
     9661 cgggcgtcgt gcccgcaaca gtgacggtga tcgaacgggt atccttgccc gagccgccga
     9721 agccgagcgc ggttagcgtg taggtggtcg tcaccttggg cgttaccgtg cgggtgccac
     9781 gcgcggccac gattccaacg ccgcggttaa gcgtgatcct gtccgcgttc accaccgccc
     9841 atttcaacgt aatggactgg ccgggttgaa ctgagttgga acccgccgtg aagctgagaa
     9901 tctgcgcgcg cggctgtgtg ggcgattgct gggccgcgga ggcggttgcc agcgcagccg
     9961 cacacgcaat cgcggtccaa agttttcgtg aaattttcat ggcgatcatt cgcgcgtggc
    10021 ctcggctccg tttctcggca ggaccataat ccactccatc ttacatgtca aaccggcgtc
    10081 cgcttgatac actggcgtcc ggatcatcag agcttcgccg atcatggcag acaccactac
    10141 aacggcgcct caactgtacg aatcgagcgg caggaatccg gtcgcaccaa tttggcatac
    10201 caccgttttt gtgctgctga ttttgggatt gagttccctg caggcaacca agctgcccat
    10261 gctcgaggcc catggccgga cgcaaatcta cctaagcgga atggcgttcg aaatcgcact
    10321 cgttctctac gtttggctgc taggcgtacg cccgcaaggc aaacgcctga gcgatttggt
    10381 tgggggaaga tgggccaaac ctatggacgt actgcgagat atcggggtgg cgcttctctt
    10441 ttggtgtgct gtgatcggct ttctgctggc catgcgtttg attctcggag caaacgccga
    10501 gggtacgcgc gcggtgaaga tgctgcttcc gcggactacc gcagagataa tctcctacct
    10561 ggcgctgtgc gtgacagcag gcttttgtga ggagctactt tttcggggct acctgcaacg
    10621 gcagtttttt gcgtggagcg ggagagtgga aatggcggtg gcattgcaag ccctggtttt
    10681 tgggcttggc cacttatatc aaggatggaa gggcgccttg acgatcacgc tctacggagc
    10741 gatgttcggc atactggcgg tgatgcgcaa aagcctgcga ccgggcatga ttcagcacat
    10801 gatgcaagac agcttcactg gagttgttgg cagcctgctc gttagacacg ggcgcttgta
    10861 gcgtcttaag gcgagtaaga ccccacgccg gctcacaagg acggagagac gggacgcgag
    10921 ccggaatcct tgaaggtgat ggcgatgaaa tggccgtcac gcgcgggcgc ggttttgaaa
    10981 acgggctttg aacctgccgg tagccagagc aaccctcctt ttaggacgtt gaccgaatcg
    11041 attcctcccg atgaggtgac cgaaactcca ccgaggcttg ccagaagaat atcggcgcga
    11101 tcattccacg gcggagcggt gacgttggca gcgatgtgcc aggcttcgac cgaaagttcg
    11161 tctgtttcga gcaggggcgt gtgcgtcgcg ggagcggccg cgcttggctc ggcaatatcg
    11221 caattctcct taggctggtc gcggaccgct tgttcgcagc gattgtgaat accgccctgc
    11281 ggacgcagca actcgatggc aacattacga agcgcaaaat cttgggagtt gctgacggca
    11341 tgggaaaagt ttccgggcga atagccgatc attggcaggc cagttccctg ccctgcggcg
    11401 ccgcgagacg acgggattgc gccgccgatg ccggttggtg gcacgctcag ataagggaag
    11461 tcgtgccggt gaaaaagagt cgattcgtgc gatggaattt cgacgaagaa tacgcggacg
    11521 taggaatttt cgaattcgag gtgatgatgg tgctcctgct ccatcgaaat gggcgcgcgc
    11581 tgcgaagctc ggccggctga ttgcgcgggc gcctgcgcta gtattggaaa cagaacggac
    11641 aggaaagtga aggcggaaaa aagaagtggc caacgcagcg aaaactttcg ggacatcgcg
    11701 gctacatctt ataacgaccg agatcctcgt cattcaaatt ctcgagccac ttccgcagct
    11761 cttcattgga gcttttttcc gacactacgc tgcttatttt ggaattcttg aggacttcat
    11821 cctcgacgaa tatcgggcaa tcgaggcgca gcgcgagggc caaggcatcg ctcgggcgcg
    11881 agtccatcga catggtttgg ccgtcgcgct ccatccagat gagagcgtag aaagtgtcgt
    11941 ccctcagatc ggaaacgaca actttttgca catggacgtt caagccggta agaacattct
    12001 taagaaggtc gtgcgtcatg ggtcgcggcg tctgcacttt ttcgatttcg agggcgatgg
    12061 cgttggcctc ataaacgccc acccagatcg gcaggattcc ggcgccctgc gcttctttga
    12121 gaaccaccac gggcatgttg gtgacggggt ccatcatcag acctcggatt ttaacttcga
    12181 gttccatgat ggcctcccgg accccgaaaa gaacggcgaa acagcggcgc tacgtaccgc
    12241 accgcattct aaaacaagtg ctcgccgaac aaactgtacg gcgccgcgcg cgtcacatgc
    12301 acctgaacgt attctcccag aagattttca cgcggcgaag tgaaatttag cacccggttg
    12361 ctagtagacc gtcccgccca ctggtgtcgc gcagcgtgat ggccgtcgac gagcgcctcg
    12421 aacacctcgc caacgagttc gccattgcgt tggacctgaa tttggcgctg cctttcctgg
    12481 agaacggcga gccggcgcga tttctccgct tccggcacag catcaagcat ggtcgaagcg
    12541 ggtgtattgg gccggggcga gtaggtgaag gcatacactc cgtcgtatcc gacggactca
    12601 agcagcgaga tggaatcctc gaaatctttt tccgactcgc ccggaaaacc aacgatcaca
    12661 tccgtggtga tgctgaccgg gcggcgcgcg gcgcgaatcc aggaaatttt ctcgaggtat
    12721 tcctcccggg tataagtgcg gcgcatggcc tgcaaaatcc gcgtcgagcc ggattgaacc
    12781 ggaagatgaa tgtggtcgca gagcgtgggt gtggcgtcga tcgcatcgac tatcgcgcgg
    12841 cagaagtcat tcggatgcga ggtggtgaag cgcacgcggc ggatgccctg gattgcagcg
    12901 acttcggaga gcaagccggc gaaatccaag tggcgcggcg aagggtcgcg ataggaattg
    12961 acggtctggc ccaacaattg aatttcggtg tagcccgcgt cggccaggcg tcgaacctcg
    13021 gataatattg cggcgctggc gcgactgcgt tccggtccgc gtgtgtgcgg caccacgcag
    13081 taggagcaag cgtaattgca tccttctatg atggtcagat aggcgcggaa tggattatca
    13141 cgccgggtca catcggtttc gaacgcttca tcggtatcaa gatcgagacc catcacgcgg
    13201 cggccgcccg attcgatttc ggcgataagt tcgggcagct tgcggtagct tgccgatccg
    13261 cacacgagac tgacccaggg cgcgcgctcg aaaatgtctt caccttcttg ctgagccagg
    13321 cagccgagaa cgccgatgat tttatctttc gagactccac gccattcgcc gaggcgcgaa
    13381 aaaactttct gcgccgcttt ttcgcggatg ctgcaagtgt tgtacagcat aaggtccgct
    13441 tccgccggat tcggcgcggc cgtgtatcca cgatcaagca gcacgcccgc cactttttcg
    13501 gaatcatgca cgttcatctg gcagccgaag gtttcgatga aaaagcgctt accagcagct
    13561 gcaccggaag ctcggccgcc gggcgacggg ccatcaaggg aatcgagcgt tcgcatcacg
    13621 gctgcggacg atggcatcgc ctcgcgggaa gcgagcgatt cgagcgcgat atcgaattgc
    13681 ctatccgaca ctgtcggttt cagtctagca caccgcaaaa agtgcgcggg actgcgcaac
    13741 cgtaaagttt caaatcgcct cgcggtgcgc taccaaccca agacccacgc aaaaatcagc
    13801 ggggccacga tggtggcatc cgattcgatg acgaatcgcg gagtgtccac actaagcttg
    13861 ccccaggtga tcttttcatt gggcacggcg cccgaatagg agccgtaact ggtggttgaa
    13921 tcgctgatct ggcagaaata agcccacagg gggacgtttt cgcggcgcag gtcctggtga
    13981 agcatgggaa caacgcagat gggaaaatct ccggcgatgc cgccgccaat ctggaaaaac
    14041 ccgagcggcg tcgcagtcgc ggtttccgtg taccagcggg ctagttcgat catatattcc
    14101 acaccgccgc gcacggtcgt cgcattctta acttttccgc tgatcacttg cgcggcgaac
    14161 atattgccaa gtgtggagtc ttcccagccg ggaacgatca tcggaagatt cttctcggcg
    14221 gcagccagca tccagctatc tttcgggtca atctggtaat actgcttcaa ttttccggcc
    14281 ttcagaacct tgaaaagaaa ctcgtgaggg aagaagcgct ggcccgcgcg gtcggccgca
    14341 taccactctt cgatcaccac gcgttcaatg cggcgcatgg cttcttcttc ggggatgcac
    14401 gtatcggtca cccggttcaa atgctgatcg agcagttcga cttcctgatc gggtgtgaga
    14461 tggcggtact gcgggacgcg cctgtagtga tcgtgcgcaa cgagattgta aacatcttct
    14521 tcgagattgg cgcccgtgca gcagatgccg tggactttat cccggcggat catctccgct
    14581 agggagaggc cgagttcggc tgtactcatg gcgcctgcga gggtgattag catttttccg
    14641 ccgctagaca gatggtccac gtaggcttcg gcagcgtcga ttacaactgc cgcgttgaaa
    14701 tggcggaagt gatgcttcat gaaactggaa attgcgttag cgctgtgctt ctcagccgac
    14761 accgtaggtt tttccttggt tgtaatcatg ggcttctcct tgtctggatg gaaattcgaa
    14821 acacccgtag cgtgaaggcg atatgcgagg agaaacggct aaaagtagct ggcggtgtac
    14881 gtcaaaagcc tgaaaaactt atgcggagaa atgtgatatt ggaacgtgcc gcaatgaaac
    14941 ttgtggagca ccgagtcata gataacacat ttctgttctt gcggcctacg gaatttttgc
    15001 gggatcgaag gagcggggtt ctttgcgccg cggaaaaacc tggaggaact ccacagtctg
    15061 gtgtaccacg gcacgcaccg aggcatctgt actataagag caactaaatt taggtcttcc
    15121 ggcttcataa gtttgaatct gcggattgcg tgtgctacgg tagatttgcc tggcacaaaa
    15181 actctttcct gggggaaaca atttccagcc tggcgtttct tcttttcgcc aacccgctca
    15241 cgcttttgga gcaaacaagc ggcgtggcac gcgttattct gggtctcttg ctgttcctat
    15301 ccatcttctc ctgggcgcta attcttcaaa agtcgtacca gctcggcgga atcgatcgaa
    15361 ggacggccag attcctgcaa atgttccgct cgggacgcgg cctatccgat cccaacacac
    15421 ttcgggcggg ggtgggcggc acgccgctca tctcggtcta tgcggcaggc tatcgggaac
    15481 tcgagcagca gatcggcgga ccgcagtcac gcgtgcccgc cagcgcaaac aagatcagaa
    15541 atgccaacgc ggtcggcgtt gagatgcagg tcgccgcggg tgaagaggtt cgacgccttg
    15601 aaaaggggat gtcgacgctg gcgaccatcg cctcagtgtc gcctttcatc ggactattcg
    15661 gaaccgtctg gggtgtgatg gacgcatttt cgggcctggg cgatgtgggc gcggcaagtt
    15721 tgcgcgcagt ggcgccaggt atctccgagg cactgatcac cacggcggcg ggattgttcg
    15781 ccgcaattcc cgcgctgatc gcttacaacc actatttgca cgggattcgc gccgtcgctt
    15841 cccgaatgtc cgatttcgcc gcggaattcg tggcgaaagt cgagacgctg tacagttcgt
    15901 agacgctcat gcctcaagcc cagcgagaag tcggcacctc cctatccgac atcaacatgg
    15961 tgccgtttat cgatgtggtt ttggtgctgc tgattatttt catgatcacc gcgccgattc
    16021 tgcagtccgg cat
//
LOCUS       DATNDT010000124         2575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1473594, whole genome shotgun sequence.
ACCESSION   DATNDT010000124 DATNDT010000000
VERSION     DATNDT010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473594"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VN788_03955"
     CDS             complement(<1..726)
                     /locus_tag="VN788_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXU19719.1"
                     /translation="MGLIEHIESKQSEHLEELKGLLRIPSVSAKTEHKPDIERAARWV
                     ADKLRAAGLDRVEMVPTKMHPLVYGESLQAPGKPTVLIYGHYDVQPAEPLDLWTTPAF
                     EPTVRGENLYGRGTADDKGQVHIHLKALESLRQASGKLPVNVKVMIEGEEEIGSISLW
                     DFVQQNRARLKADALIVSDTSMLAKGVPSITYGLRGLNYYELEITGPAQDLHSGAFGG
                     AVPNPTTILAEMIAKLHDRDFRVA"
     gene            879..1241
                     /locus_tag="VN788_03960"
     CDS             879..1241
                     /locus_tag="VN788_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19720.1"
                     /translation="MTPETQATVQRRSSRVFTRIAIQAKGKNDHGRRFHSKSQTIVIN
                     AHGGLLYLGESLSVGTEIILVNPATEEEQECRIVYVGDTSDKGTRIGVEFLSPSPHFW
                     GVEFAQQDWPANPPSSQD"
     gene            1389..>2575
                     /locus_tag="VN788_03965"
     CDS             1389..>2575
                     /locus_tag="VN788_03965"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6351 family protein"
                     /protein_id="HXU19721.1"
                     /translation="MSAKKSRLVFCVLGMFVAGASAASVRAQSDAGGTIAPVQKCADL
                     VNLHIPGTNVTITKAEAIPTAPPGTIRASALSPATIAVAIPSYCRADGVIDPRTGFDG
                     KPYAIGFAIALPDKWNGRFLFQGGGGLNGSINPPYGGQATGEAPGLARGFAVVSTDTG
                     HKGAVFDAAFMKDQLASLDFAYIAVGKVAVLAKQIIAQYYGQPAKHSYYDGCSTGGRE
                     GMLMSQRYPTYFDGIVSGDPAMRTGYSNIGLTWARVAFAQMAPKDTSGKPDPTKDFSA
                     GDKKLLADAIMKTCDDKDGLKDGMIYNPEACNFDPEIIKCKGAKTDSCLTAAQVGALR
                     KAFAGPKDSRGNQIYARFPWDTGYLSGVGGPFGSFLPSAGPNILASVQPPADLSIDEL
                     VANV"
BASE COUNT          520 a          715 c          810 g          530 t
ORIGIN      
        1 cgccacgcga aaatcgcgat cgtgaagctt cgcgatcatt tcggccaaaa tcgtggtggg
       61 gttgggaacg gcgccgccga aggctccgga gtgcaggtcc tgcgccggtc cggtaatctc
      121 caactcgtaa tagttcaaac cccgaaggcc ataggtgatc gaaggaacgc ctttggccag
      181 catggaggta tcggagacaa tcaaggcgtc ggccttgaga cgtgcgcggt tttgctgcac
      241 gaaatcccac aagctgatgc tgccgatttc ctcttcgccc tcgatcatca ctttcacgtt
      301 gacgggaagt ttgccagagg cttgccgcag cgactcgaga gctttgaggt ggatgtgcac
      361 ctgccctttg tcgtcggccg tgccgcgacc gtaaaggttt tctccgcgga cggtaggctc
      421 gaaggcaggg gtggtccaca aatcgagagg ctcggcgggc tgcacgtcgt aatgtccgta
      481 aattaagacc gtgggcttgc cgggcgcttg aagggattca ccatacacaa gcgggtgcat
      541 cttggtggga accatttcca cgcgatcgag ccctgcggcg cggagtttat cggccaccca
      601 ccgcgcggcg cgctcgatat cgggcttgtg ctcggttttc gcactgacgc tgggaatacg
      661 gagcagcccc ttcagctctt cgagatgctc ggattgtttc gattcaatat gctcgatcag
      721 acccattcag agctcctgta gctaattttc gccgcaatcc gcggtgccat cttatccgac
      781 gatgagggtt tcaagcgaat ttttggtaac tgccgttttg ggcacgcgcg cgccgctctc
      841 gaattgactc ggctcgggcg gcgttgcacg atagcgttat gacgccggaa acacaagcga
      901 ccgtgcagcg acgcagttcg cgcgtcttca cgcgaattgc gattcaagcg aagggtaaga
      961 acgaccacgg acgacgcttc cactcgaaga gccagaccat agtgatcaat gcgcacggcg
     1021 gactgctcta tctgggcgaa tcgctcagcg tggggacgga aatcattttg gtgaatcccg
     1081 ccacagagga agagcaagag tgccgcatcg tgtatgtggg tgacacgtcg gacaagggaa
     1141 cgcgcatcgg cgtggagttt ttgtcgccca gcccgcattt ctggggcgtg gaatttgcgc
     1201 agcaggactg gccggcaaac ccaccttcgt ctcaagatta accgggcctc aactccgagt
     1261 gaatcacgcc gccgcgcgca aagcgcggcg tttcgcttca aacatctgct tgcatccccc
     1321 tgcggggtga ctacaatggc gtagtttttg ggcggaaatg cgcgatgccg cgaaggagac
     1381 gcgccgtgat gagcgccaag aaatctagat tagttttctg cgtgttgggc atgtttgtgg
     1441 cgggagcttc ggctgcgagc gttcgagcac agagtgatgc gggcggaaca atcgcgccgg
     1501 ttcaaaagtg cgctgacctg gtgaatctcc acattcccgg cacaaacgtg accatcacga
     1561 aggcggaggc gatcccgacc gctccgccgg ggacgattcg cgcttcggca ctttctccgg
     1621 ccactatagc agtggcaatt ccgtcatatt gccgcgcgga cggcgtgatc gacccgcgaa
     1681 cgggctttga cggcaagcct tacgctatcg ggttcgccat cgcgctgccc gacaaatgga
     1741 atgggcgatt cttgtttcag ggaggcggcg ggctcaacgg ctcgatcaat cctccttatg
     1801 gcgggcaggc cacgggcgag gcgcccggac tggcgcgagg cttcgcggtt gtttccaccg
     1861 ataccggaca caagggcgcg gtgttcgacg cggctttcat gaaggatcaa ctcgcgagcc
     1921 tcgattttgc ctacattgcg gtgggcaagg ttgcagtgct cgcgaaacag atcatcgcgc
     1981 agtattacgg gcagcccgcg aagcattcat attacgacgg ctgctcgacg ggcgggcgcg
     2041 agggcatgct gatgtcgcaa cgctatccga cgtactttga cgggattgtt tcaggcgatc
     2101 cggcgatgcg caccggctac tcgaatatcg ggctgacgtg ggcgagagtc gctttcgcgc
     2161 agatggcacc aaaggacacg tccggcaagc ccgatcccac gaaagacttt tcggccggcg
     2221 acaagaagct gcttgccgat gcgattatga agacttgcga tgacaaggat ggactaaaag
     2281 acgggatgat ctacaacccg gaggcttgca atttcgatcc tgagattatc aagtgcaagg
     2341 gagcgaagac ggattcgtgt ctgacggcgg cacaggttgg cgcgctgcgg aaggctttcg
     2401 ccgggcccaa ggattcgcgc ggaaaccaaa tctacgcgcg gttcccgtgg gataccgggt
     2461 atctttcagg cgtaggcggg ccgttcggca gttttctgcc gagcgccggg ccgaatattc
     2521 ttgcttcggt gcagccgccg gctgatttga gcatcgatga actggtcgca aacgt
//
LOCUS       DATNDT010000125        13235 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3272860, whole genome shotgun sequence.
ACCESSION   DATNDT010000125 DATNDT010000000
VERSION     DATNDT010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13235
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272860"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3136
                     /locus_tag="VN788_03970"
     CDS             <1..3136
                     /locus_tag="VN788_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXU19722.1"
                     /translation="ECAALVRSIRTGELDRILIPEAPLDILAQQIVAACSAEDWSEDD
                     LFALVRRAYSYRTLVRKDFDEIIGVLSEGIAARRGRYGAYLHRDGVNHRLRGRRGSRL
                     AAITSGGAIPDNALYTVRAEPEGFTVGTVDEDFAVESLRGDIILLGNTSWRIRRVQSG
                     LVLVEDAKGAPPNVPFWRGEAPSRTAELSQQVAAIRENLSALTEGMAPDAVRGNTGQG
                     SAAAEWLQQECGLDRAGAEQTVEYIVAGRAVLRAVPSQQTVIAERFFDESGGMQLVIH
                     APFGGRINKAWGLALRKRFCRSFNFELQAAATDNGLNISLSEQHSFPLEDVFRFLHPE
                     SVQEVLEQAVLTSPIFTTRWRWVAGRALALLRFMGGKKVPPQIQRMRSDDLLAAVFPE
                     ALACQENISGEIQIPDHPLVRETMKDALTEAMDVEGLKRVLEGIRAGQIRCVAVDTPV
                     PSQFSHEILNANPYAYLDDAPLEERRARAVEMRRALPEAVLSEIGRLDPSAIAEVRGE
                     AWPDVRDADELHDVLQTLVLLPASFSQDVSPLVESAGKSMREWSTFFDELTAAGRAAR
                     AFAGNRAYWVAAEKASSFASILPEARFDSPLPSVEKPPLSREDAVLGCVVGWMAHAGP
                     VTTIEVSDFLGLGAADVEKAFLRLEAGGSILRGQFTNSGSTEWCERRLLARIHRLTLG
                     ALRSQIQPLTAAEFMRWLLRWQHLAPGTQVLGEHGTFEVLRQLQGFEAPANAWERQIL
                     ARRIANYDPRVLDQLCLSGAVGWGRLSPHPATIAEYAATNRRVIPTSVAPIAFFVRED
                     SEWMIQRHSPEGQGVPHGLSEAARDALNYLRQRGASFFTDLVRGTQRLKSEVEAALWE
                     LVAAGMITADGFDNIRALIDPKRRSGQGRGQTARPRHSVGRWSLLYTEEVPERAQLAE
                     ATCWMLLRRYGVVFREMTAQETIAPRWRELLIAFRRLEDRGQVRGGHFVSGFMGEQFA
                     LPIAVDSLRAMRTDSLPGESLTISAADPLNLTGILLPGDRISSVSGKTITLRDGIYSA
                     GDEAASGFSDYVAV"
     gene            3275..3922
                     /locus_tag="VN788_03975"
     CDS             3275..3922
                     /locus_tag="VN788_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007684362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU19723.1"
                     /translation="MGSLRILVADDHDLMRRGIKTIIASRKDWEVCGEAVTGSQAVAK
                     AQELKPDIAILDISMPELNGIEAAKRIRKMSAKTEILVLSAHYSDQIVRDVMEAGIRG
                     YLVKSDSSRDLSTALDSLATHRPFYTSSVSEIMLDEGRSSTKAPMATKSRITSRENEI
                     VQLLAEGKSSKDIAVFLNISVKTVETHRANIMRKLELHSVTELVRYAIRNSLVEA"
     gene            complement(3957..12767)
                     /locus_tag="VN788_03980"
     CDS             complement(3957..12767)
                     /locus_tag="VN788_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020482746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HXU19724.1"
                     /translation="MVSAPPAPNVVVEIEEPIVAELFSADRLEQHAESLAAAQTVAAA
                     AAEGGPSLIERTEGNARSLIEYYRATARALQREQIITPAEQWLVENFYIVEDQLREIR
                     DDLPIGYYRRLPKLASGHLIGYPRVYGIAWAFVAHTDSRLDPHVFRRFINAYQRVQPL
                     SIGELWAVAITLRIVLVENLRRLAGRIVFSRQARHQADQLADALLGTGGQSALSPAAA
                     LRQFEKKPLDRAFAVELIQRLRDLDPKVGPILLWLDERLAAQGTTADDIVRAEHQQQA
                     AMSVTVRNVITSMRWTSEFDWHTFFESVSLVDQTLRKGSKFGEMDFSTRDAYRHAIED
                     LSRGSELSELEITELVVEHTRDAAATGADHDHQPHDETFSDPGYYLIAQGRTAFERQI
                     GFRVSKRRAFLRWYMRAAVRGYLGTIALFTGIILAFPVARSLQLGLRIPEIIILTVLA
                     VVPASELAIALINRLVTNLFKPRTLPRLEFEKGVPADLRTIIVIPTLLSSTGGIAEQI
                     HQLEIHYLANQDGDLRFGLLSDWIDAATETLPSDQELLAVAAEGIANLNKRYGPAKDG
                     GNRFFIFHRKRVWNESEQKWMGWERKRGKLHELNLLLRGSTNTTFIPLAGHAIESIPD
                     VRYVITLDADTRLPRGTAGRLAGAMAHPLNRPKFSNEQSRVVAGYGIIQPRVTPSLPM
                     DNRGSIFQRVFSGPSGIDPYSSAVSDVYQDLFHEGSYTGKGIYDIDAFEAALAGKVRE
                     NVVLSHDLFEGIFARAALVSDIELFDDFPSHYEASAARQHRWARGDWQLLPWILGWGT
                     RLTDGRHYKIPVIGRWKMLDNLRRSLLAPCVFLAFLASWLVMGSFPWLWIRFILATIV
                     IPPLIPFLAGLNPRIGGAISNRNYFRDLVSDLSLGLSQAGLALTFMAYQAWLMSDAIL
                     RTLFRLFITRKNLLEWVTAAQAKHRIDLDIYGSFQRMFGGIAFAVAAFIAVWFGHRQA
                     IPIAIPFVLLWLSAPAVAHWISLPPKPTEDETLSAVDTSTLRTIARRTWRFFETFVGS
                     EDHWLPPDNFQEDPKPVVAHRTSPTNIGLYLLSTVAARDFGWLGTHEMLERIEPTLQT
                     MGGLEKFRGHLCNWYETLSLQPLEPKYVSSVDSGNLAGDLLTLAAGCRQLIHQRAVAP
                     TILEGVQDSIVLLREALDKLSDTARLHAVTPKQLREAVNALADSTRPFTGDAADWGAR
                     ILELFRRAQTVTDMAQALAEELRRDAESELNIWAQAAKATVESHLRDARVLLPWLRLV
                     EGNPLGISTNGSARPAEWAEIEPFFGIDSTLADAPERFVAAAGELADLRDHLVHEGPS
                     QKDQLERIEALIGALKEGSAEAAALRARLRALTATSENMFYAMDFGFLFDPDRKLLSI
                     GYRLSEGTLDEGCYDLLASEARLASFIAIAKGDIPASHWFKLGRPLTQIGRGSALISW
                     SGSMFEYLMPAIIMPSPANSLLSQTYHQIVLRQIEYGAERGVPWGMSESAFSARTIEL
                     VYHYSGFGVPGLGLKRGLNEDLVIAPYATALAAMIEPTAAAKNFERMARAGAEGAYGF
                     YEALDYTRSRLPEGEQVAVVRTYMAHHQGMSIVALDNVVSAGIMREKFHSQPIVQASE
                     LLLQERTPRDVLVTMPRAEEVSAGPYVRDLLPPTTRRFKTPQEIAPRTALLSNGRYAV
                     MLTATGSGYSRWRDIAITRWREDVTRDCWGSYFYLRDEYTASIWSAGYHPTATEPDFC
                     EAIFHEDHAEFMRRDRSITTSVEVMVSSEDDAELRRISISNGGIRARDIQVTSFAEIA
                     LTTQAADAAHPAFSNLFVETEFESELGVLLATRRKRSDDEASVWAAHLLVVDGGETVG
                     DLQYETDRVRFIGRTRTPRNPVSIMDGRPLSNTVGPVLDPILSLRRTVRVPPGRTVHL
                     IFATIVGPTREAVLDIADKYRDAKTVDRTRTLAWTQSQVQLRHLGIGAEEAHLFQSLA
                     NAVLYSDRSLRPPADMLGRTTIDKHALWSQGISGDLPIVLARIEDTEDLDLIRQLLRA
                     HEYWRMKQLSADVVIINEKTSSYIQELQGSLEALVRGSQLRLSPDSSEASGKIYLLRA
                     DLVSLHVREQIEAAARVVLVARRGPLSDQVIRAQQDQPSVPSPDRAARIGKRMDVPLP
                     QQELQFFNGLGGFTQDGREYIVVLSEGLRTPEPWINVIANPEFGFTVSESGSGFTWFG
                     NSHENQLTPWSNDHVTDSAGEAIYLRDEYSGEVWTPTALPIRDENATYLSRHGQGYSR
                     FHHGSRGILIDLVQFVPLEDPIKISRLTLQNESGRSRRISVTAYAEWVLGTSRSASAP
                     HIITEVDSRTGALLARSAWAGEFGGRIAFADLAGRQTALTADRAEFLGRNGAHEFPEA
                     LEFGRPLSGRVGAALDPCAALQTVIELRPGARVEIKFFLGQAESKKQVVELLERYRAA
                     DLDKTFSQVTGYWNDLLATVQVATPDPSLDLLLNHWLLYQTLSCRVWARAAFYQLSGA
                     YGFRDQLQDIMALTVAKRGIAREHLLRAAKHQFFEGDVQHWWHPPSGRGIRTRMSDDL
                     LWLPYVVTNFIEATGDMTVLEEVIPFIEGPLLADGQKESYFEPRESGTSATLFEHCAR
                     ALDRSLPVGSHGLPLMGTGDWNDGMNQVGQNGKGESVWLGFFLHSVLWEFSKLADARG
                     EHKRAETWRLHVSALKAALEREGWDGEWYRRAYFDDGTPLGSAADQECRIDSIAQSWG
                     IMTGAAERARGARAMAAVDRLLVRRGDNLVLLLTPPFNHTTHNPGYIRGYVPGIRENG
                     GQYTHAAVWTVTAFAAMSDGDKAHELFRSLNPINRTSSRAGVQRYKVEPYVVAGDVYA
                     EPPHVGRGGWTWYTGAAGWLYRAGLEWILGFRLRGMVLGIDPCIPRNWARYSVKFRYH
                     SSIYDIQIENPSGVTRGVVLTELDGKLLAKAANIPLANDGAVHLVRVVLG"
     gene            complement(12966..>13235)
                     /locus_tag="VN788_03985"
     CDS             complement(12966..>13235)
                     /locus_tag="VN788_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19725.1"
                     /translation="GTYRDVVRQLRRQPVEIPVIVVCAPGCSQEYRDYLAALNMRAFD
                     YICYPYRKADLERILHSAISTQHASMRSRDTYSTLEAGSGKLRAV"
BASE COUNT         2851 a         3753 c         3936 g         2695 t
ORIGIN      
        1 cgagtgcgca gctctggtgc gttccatccg gacgggcgag ctggatcgca ttcttattcc
       61 cgaagcgccg ctggatattt tggcacagca gattgtggcc gcgtgctcgg cggaggattg
      121 gagcgaggat gatttgttcg cgctggtccg ccgcgcttat tcgtaccgta ctttggttcg
      181 aaaagatttc gatgaaatta tcggagtgct ttcggaggga atcgccgcac ggcgcggacg
      241 ttacggcgcg tacctgcatc gcgatggagt gaatcatcgc ctgcgcggac ggcgtggaag
      301 ccggctggcg gcaatcacga gcggaggggc gatcccggat aacgctctct acaccgttcg
      361 agccgagccg gaaggcttta ccgttggcac ggtggatgag gatttcgccg tcgaaagcct
      421 gcgtggagac atcatccttc tcggaaacac ttcgtggcgg atacggcgcg tgcaatcggg
      481 actggtgcta gtcgaagacg ccaagggagc gcccccgaac gtgccttttt ggcgcggaga
      541 agcgccgtcg cgcaccgcgg agctttcgca gcaagtcgcc gcaattcgcg aaaatcttag
      601 tgcgctcacg gagggaatgg cgcccgatgc ggttcgtggg aacacggggc agggatctgc
      661 cgcagcggaa tggctgcaac aggaatgcgg gctcgaccga gccggcgcgg aacaaacggt
      721 ggagtacatc gtggccggcc gcgccgtatt gcgcgctgtc cccagccagc aaacggtgat
      781 cgctgagcgg tttttcgatg aaagcggcgg catgcagttg gtgattcacg cgccatttgg
      841 agggcgaatc aacaaagcgt ggggactggc gctgcgaaaa cgcttttgcc gttcattcaa
      901 ctttgagctg caagcggccg caaccgacaa tggactgaat atctcgctca gcgagcagca
      961 cagctttccg ctggaagatg tatttcgttt tcttcacccg gaatccgtgc aggaagtact
     1021 ggaacaggcg gtgcttacgt cgccgatttt cacgacacgc tggcggtggg tggcgggccg
     1081 tgctttggca ttgctgcgct tcatgggcgg gaagaaagtg ccaccgcaaa ttcaacggat
     1141 gcggtcagac gatcttctgg cggctgtttt ccccgaagcg ttggcgtgcc aggaaaacat
     1201 ttcgggagag attcaaattc ccgatcatcc gctggtgcgc gaaacgatga aggacgcgct
     1261 caccgaagcg atggacgttg agggattgaa acgagtgctc gaaggtattc gagcggggca
     1321 aattcgatgc gttgccgtgg acacccccgt gccctcgcaa ttttcccatg agattctgaa
     1381 tgcgaatccc tatgcgtatc tggatgatgc gccactcgag gagcggcggg cgcgggcggt
     1441 ggagatgcgg cgcgcgttgc cggaggctgt gctctccgaa attgggcgcc tggacccgtc
     1501 ggcgatcgcc gaggtgcggg gagaagcatg gccggatgta cgcgacgcgg atgagcttca
     1561 cgatgtgctg caaacacttg tgcttctgcc cgcaagtttt tcgcaagatg tttcgccact
     1621 tgtggagtcc gccggtaaat ccatgcgaga gtggtcaact ttctttgatg agcttactgc
     1681 tgcgggccgc gctgcgcgcg ccttcgctgg caatcgcgct tactgggttg cggcggagaa
     1741 ggcgtcatcg tttgcgagca ttcttccaga ggcgcgtttc gattcaccgc ttccgagcgt
     1801 cgagaagccg ccgctatcgc gcgaggacgc ggtgttggga tgtgttgtcg gatggatggc
     1861 gcacgcgggg cctgtgacca cgattgaggt gagtgatttc ttgggcctcg gggctgcgga
     1921 tgtggagaag gcgtttctgc ggctggaggc cggcggctcc attttgcgcg ggcaattcac
     1981 aaatagcggc tcgactgaat ggtgcgaacg ccgcttgctg gcgcgcatcc accgcttgac
     2041 gcttggcgcg ttgcgcagcc agattcagcc gctcactgcc gccgagttca tgcgatggct
     2101 gctgcgctgg caacatttgg cgccgggaac gcaggttctg ggtgagcatg gaactttcga
     2161 agtactgcgg cagcttcaag gattcgaagc gccggccaat gcttgggaga gacaaatcct
     2221 cgcccgcaga atcgccaatt acgacccacg tgttctggac cagctctgcc tatcgggagc
     2281 cgtgggttgg ggccggctgt ctcctcaccc ggccacgatc gcggaatacg cagcaacgaa
     2341 tcgccgcgtg attcctacga gcgttgcgcc gatcgcgttt ttcgttcgcg aagattccga
     2401 gtggatgatc cagcggcact cacctgaggg gcaaggcgtg cctcatgggc tgagcgaggc
     2461 ggcgcgcgat gcactgaact atttgcgcca gcgcggcgct tcatttttca ccgatcttgt
     2521 tcgcggcacc cagagattga agtcggaagt tgaagcagcg ctgtgggaac tggtggctgc
     2581 aggaatgatt accgcagacg gcttcgataa tatccgcgcg ctgatcgatc cgaagcgccg
     2641 ctcgggacaa ggccggggac aaacggcgcg gccacggcac agcgttgggc gctggtcgct
     2701 gctctatact gaagaggtcc ctgagcgcgc acaattggcc gaggcaacat gctggatgtt
     2761 gctacgccgc tatggagtag tgttccgtga aatgaccgcg caggaaacga ttgctccgcg
     2821 ctggcgcgaa ttgctcattg cgtttcggcg tcttgaggat cgcggccagg tgcgtggcgg
     2881 acacttcgtc agcggattta tgggggaaca attcgcgctg cccatcgcgg ttgactcctt
     2941 gcgcgccatg cgtacggatt cattgccggg agaaagcctg acgatctctg cggccgaccc
     3001 attaaacttg acagggattt tgctgccggg tgatcgaatt tcgtcggtat cggggaagac
     3061 gatcactctg cgagacggca tatattctgc aggtgacgag gcggcgtcag gcttttccga
     3121 ctatgtagca gtttaaggac tgtcgcgcgg aacctacagg tgttatcccc ctctttgtca
     3181 gtgagatacc gtatggacac aaagggcgtg cggcggcaca ctacggcgtc aatttcagat
     3241 ttcaagtgac gccgattgtt ccaaggaggc tcttgtggga tcactcagaa ttcttgtggc
     3301 ggatgaccac gatctgatgc ggcgcggcat taagacgatt atcgcatcgc gcaaagattg
     3361 ggaagtttgc ggagaggccg tcaccggttc ccaggccgta gccaaggccc aggagttgaa
     3421 acccgatatc gccattctgg acatttcgat gcccgagctt aatggcatcg aggccgcgaa
     3481 gagaattcgg aaaatgtcgg cgaagaccga gattctggtt ctttcggcgc actattcgga
     3541 ccaaattgtc cgagatgtaa tggaggcggg gatacgcgga tacctggtga agtcggattc
     3601 cagccgcgac ctctcgaccg cccttgacag tctggccacc caccggccgt tttacacatc
     3661 ttcggtatcc gaaatcatgc tcgacgaggg ccgctcaagt accaaggccc caatggcgac
     3721 caagtcccgc atcacttcgc gggaaaacga gatcgtccag ctgctcgcag agggaaaaag
     3781 cagcaaggac attgccgttt tcctgaacat cagcgtaaag accgtcgaga ctcatcgcgc
     3841 caatatcatg cgcaaacttg aactacacag cgtgacggaa ctggtgcgct acgcaatccg
     3901 caacagcctc gtggaggcgt gacaagcaga cagaacaata atttcccgcc agggagtcag
     3961 ccgagcacga cacgcacaag atggactgcg ccatcattgg cgagaggaat gtttgccgct
     4021 ttagctaaga gcttgccgtc cagctccgtg aggaccacgc cgcgagttac cccagacggg
     4081 ttttcgattt gaatatcgta aatcgacgag tggtagcgaa actttacgga gtagcgagcc
     4141 cagttcctgg gaatgcacgg gtcaatgcct aagaccatgc cgcgcagtcg aaagccgagt
     4201 atccattcca agccagcacg atagagccaa ccagccgcac ccgtatacca cgtccatccg
     4261 ccacgtccaa cgtgaggagg ctcggcataa acatctccgg caacgacata cggctcgacc
     4321 ttatatcgct gaacgcccgc gcgtgaactg gtgcggttga tgggattcag actacggaaa
     4381 agctcgtggg ctttgtcgcc atcgctcatc gcggcaaagg cggtcaccgt ccagacggcg
     4441 gcatgcgtgt actggccgcc gttttcgcga atgccgggga catagcctct gatgtagccc
     4501 ggattgtggg tcgtgtggtt aaaaggcggc gtcaaaagaa ggacgagatt gtcgccccgc
     4561 cggaccagca aacgatccac tgcggccatg gcacgcgctc cccgcgcgcg ttccgccgcg
     4621 cccgtcatga tgccccacga ctgggcaatg gagtcgatac ggcattcctg gtcggctgcg
     4681 gaacccagcg gagttccatc gtcaaaataa gcccggcgat accattctcc gtcccagcct
     4741 tcacgctcga gcgcggcctt cagtgcgctg acgtgtaatc tccaagtctc ggcgcgcttg
     4801 tgttcaccgc gggcatcagc cagtttggaa aattcccaaa ggacggaatg aagaaagaat
     4861 ccgagccaaa cactttctcc ctttccgttc tgtccaacct ggttcattcc gtcgttccag
     4921 tcccccgtgc ccattaaggg cagcccatgg ctgccgacgg ggaggctgcg gtccagagcg
     4981 cgcgcacagt gttcgaagag agttgcactc gtcccggatt cgcgcggctc aaaataggac
     5041 tctttctgac cgtcggccag gagcggcccc tcgataaagg ggatcacttc ctccagcacg
     5101 gtcatgtcgc ctgtagcttc gataaagttg gtcaccacgt agggaagcca aagcagatcg
     5161 tctgacattc gcgtgcgtat tccacgaccc gacggagggt gccaccagtg ctggacatcg
     5221 ccctcgaaga actggtgctt ggctgcacga agcaaatgct cgcgggcgat tcctcgcttc
     5281 gcgaccgtga gcgccattat gtcttgaagc tggtcgcgga aaccatatgc gccgcttagt
     5341 tgatagaacg ccgcccgggc ccaaacgcgg caggagagag tctgatacag gagccaatgg
     5401 ttcaggagca aatccaaact gggatccggc gtcgcaactt gaacggtggc caaaagatca
     5461 ttccaatagc ctgttacttg actgaaggtt ttgtcgagat ccgcggcgcg ataccgttca
     5521 agcaattcga caacttgttt cttgctttcg gcttgaccca ggaagaattt gatttccact
     5581 cgcgcgccgg ggcgaagctc gatcaccgtc tgaagcgcgg cgcacggatc gagcgcggct
     5641 cccacccttc cggaaagcgg ccggccgaat tcgagcgctt ccgggaattc gtgcgcgccg
     5701 ttgcgaccga gaaattcggc gcgatccgcg gtaagagcag tttgacggcc ggccaggtcg
     5761 gcaaacgcaa ttcgtccgcc gaattcgccc gcccatgcgc ttcttgcgag aagcgcgccc
     5821 gtccgcgagt ccacctccgt gatgatatga ggagctgacg cgctgcgaga ggttcccagg
     5881 acccactctg catacgctgt gacggaaatg cgtcgactgc ggcccgattc attctgcaag
     5941 gtaagccttg aaatcttgat cggatcctcc aacggaacaa attgcacgag gtcgatcagg
     6001 atgccgcgtg agccatggtg gaaacggctg taaccttggc catggcgtga aaggtaggtt
     6061 gcgttttcgt cgcgaatcgg aagggccgtc ggagtccaaa cctcacccga gtattcatct
     6121 cgaaggtaga tcgcttcgcc cgcagaatcg gtaacgtgat cgttggacca gggcgtaagt
     6181 tggttctcgt ggctattccc gaaccacgtg aatcccgatc cggattccga gacagtaaat
     6241 ccaaattccg gattggcgat cacattgatc cagggctccg gagtcctcag tccttcgctc
     6301 agaactacga tgtactcgcg accatcttgg gtgaagccac ccagaccatt gaaaaattga
     6361 agctcctgtt gagggagagg gacatccatg cgcttgccta tgcgtgcggc gcgatcgggc
     6421 gagggcactg aaggctggtc ttgctgcgcg cgtatcacct gatcggatag ggggcctcgc
     6481 cgcgcaacaa gaaccacgcg cgcggctgcc tcaatctgtt cacgaacgtg cagcgaaacc
     6541 aagtccgcgc gcaaaagata tattttccca ctcgcttcac ttgaatcggg agaaagccgc
     6601 aattggctgc cacgaaccag cgcctcgagg gaaccttgga gctcctgaat ataggatgag
     6661 gtcttctcgt tgataatcac cacatcggcg gacaactgtt tcatgcgcca gtactcgtgc
     6721 gctcgcagga gctggcggat caaatcgaga tcctcggtat cctcgatgcg tgccaagacg
     6781 atgggtagat cgcccgaaat gccctgcgac cataacgcgt gcttatctat cgttgtacgc
     6841 cccagcatat ccgcaggcgg acgcagggaa cggtcggaat acaggaccgc attcgccaga
     6901 ctctgaaaca agtgcgcttc ctccgccccg ataccgaggt ggcgaagttg aacttgcgac
     6961 tgcgtccaag cgagcgtgcg tgtgcggtcc acggttttcg cgtcccgata tttatcggcg
     7021 atatccagca ctgcttcccg cgtggggccg acaatcgtcg caaagatcaa gtgcaccgtc
     7081 cttccagggg gcactcgaac ggtccgccgt agactcaaaa tcggatcgag cacgggacct
     7141 actgtattcg acaaagggcg gccatccata attgacactg ggtttcgtgg agtacgagta
     7201 cgtccgatga agcgcacgcg gtcggtttcg tactggagat ctccaacggt ttctccaccg
     7261 tcgacaacca acagatgggc ggcccagacc gatgcctcat catcggaacg ctttcgacgt
     7321 gtcgccagca gcacaccgag ctcggattcg aattccgtct ccacgaacaa gttcgaaaat
     7381 gccgggtggg ccgcatcggc ggcctgagtg gtcagcgcaa tctccgcgaa ggaagtcacc
     7441 tgaatatccc gcgcacgaat tccgccattt gaaatcgaaa tgcgccgcaa ttccgcgtcg
     7501 tcttcagaag agaccatcac ctccacggaa gtggtgatcg agcggtctcg ccgcatgaat
     7561 tccgcatggt cttcatggaa gatcgcttca caaaaatccg gttcggtagc cgtcgggtga
     7621 tatccagccg accaaatgct ggccgtgtac tcgtcgcgga ggtaaaaata gctgccccag
     7681 caatctcgag ttacgtcttc tcgccaccgg gtaatcgcga tgtctctcca acggctgtag
     7741 ccggatccgg tcgcagtcag cattaccgca tagcgaccgt ttgatagcag cgcggttcgc
     7801 ggggcgatct cctggggggt cttgaaacgg cgagtcgtag gaggtagaag gtcgcggacg
     7861 taaggaccgg ccgagacctc ctcggcgcgt ggcatcgtca ccagcacatc cctcggcgtg
     7921 cgctcctgta ataggagttc ggacgcctgg acgatcggct gcgaatgaaa tttttcgcgc
     7981 atgatgcccg cgctgacgac attgtccagc gcgacaatag acatcccctg atgatgagcc
     8041 atataagtgc ggacaacggc gacttgctcg ccttccggta agcgagagcg ggtgtagtcc
     8101 agggcctcgt aaaatccata cgctccctcc gcccctgcac gggccatgcg ttcgaaattt
     8161 ttggccgccg ctgtgggctc aatcattgcg gcgagcgccg tggcgtacgg ggcgattacc
     8221 aggtcctcgt tcaggccgcg cttcagcccc agccctggca cgccaaagcc ggagtagtga
     8281 tagaccagct caatggtccg cgctgaaaaa gccgactccg acattcccca tggcactcca
     8341 cgctccgcgc cgtattcgat ctgccggagt acgatttggt gatatgtctg gctgagaagg
     8401 ctattagccg gcgacggcat gatgatcgcc ggcatcaggt attcaaacat cgatccggac
     8461 caggaaatga gcgccgaacc ccggcctatc tgcgtgaggg ggcggcctag cttgaaccag
     8521 tgcgatgcag gaatatcgcc tttggcgatg gcgataaagc tggccagtcg tgcctccgaa
     8581 gcgagcaggt cgtaacagcc ttcgtcgaga gttccttcgg atagacggta cccgatggag
     8641 agcaacttgc gatcgggatc gaacaggaat ccaaaatcca tcgcgtaaaa catgttttct
     8701 gaagtggcag tgagggcccg tagtcgagca cgcaatgccg cggcctccgc ggacccttct
     8761 ttcagcgccc caatcagagc ttctatccgt tctaattggt ctttttgaga cggcccttca
     8821 tgaaccagat ggtcacgcaa gtcggcaagt tcgccagccg cagctacgaa tcgctcggga
     8881 gcgtccgcaa gagtgctgtc gatcccgaaa aacggttcga tctccgccca ttcggctggc
     8941 ctggctgacc cgttggtgga gatgcccaga ggatttcctt caacgagtcg caaccaagga
     9001 agcagcaccc tcgcatctcg caaatggctc tcaacagttg ctttcgccgc ctgagcccaa
     9061 atgtttagct cggattcggc atctctccgg agctcttcag ccagcgcttg cgccatatcg
     9121 gtaaccgttt gcgccctccg gaataattcc aggattcggg ctccccaatc tgcggcatcg
     9181 ccagtaaatg gtcgcgtgga gtcggcaagc gcgttcaccg cttcgcgaag ctgcttcggc
     9241 gtaacggcgt gaagccgggc cgtgtcgctt agcttatcga gagcctcgcg gagaagaacg
     9301 atcgaatcct ggacgccttc aaggatggtc ggcgccacgg ctcgctgatg gatcaactgg
     9361 cggcagccgg ccgcaagtgt cagcaggtcc ccagcaaggt tgccgctatc caccgaggaa
     9421 acatatttcg gttcgagcgg ttgaaggctc agcgtttcat accaattgca caaatgcccc
     9481 cgaaactttt caagcccgcc cattgtttgc aacgtgggct cgatgcgctc aagcatttcg
     9541 tgcgtcccca accaaccgaa atcgcgcgcc gccactgtcg aaagaaggta caggccgata
     9601 tttgtcggag aagtgcggtg agcgaccacc ggcttgggat cctcctgaaa attatcaggt
     9661 ggcagccagt ggtcttccga cccaacgaag gtctcgaaga acctccacgt acggcgcgca
     9721 atcgtgcgca gcgtactggt gtcgacggcg gatagggttt catcttccgt cggtttcggc
     9781 ggaagactga tccagtgagc tacggcgggc gctgagagcc acaacaaaac aaatggaatg
     9841 gcgatgggta tcgcttgccg atgtccaaac cacaccgcga tgaaggccgc aacggcgaag
     9901 gcgatccctc cgaacatccg ttggaaactg ccgtagatgt ccaagtcgat gcgatgcttt
     9961 gcttgcgccg ctgtcaccca ctccagcaga ttcttgcggg tgatgaacaa tcggaaaagt
    10021 gttcgaagaa tagcgtccga catcaaccaa gcctggtacg ccatgaacgt cagggcgagg
    10081 ccagcctgag acaatcccag ggagagatcg gaaaccaagt cgcggaagta attccggttt
    10141 gatattgcgc cgcctattct tgggttgagt cccgccaaga aagggatcag gggtgggatt
    10201 acgatcgtgg ctaaaataaa tctgatccag agccaaggga acgatcccat tacgagccaa
    10261 ctcgcgagga atgccaggaa cacgcacggc gcaagcagag agcgacgcaa gttgtctaac
    10321 attttccatc ggccaataac cggaatcttg tagtggcggc catcggtcag acgtgttccc
    10381 cagccaagaa tccaaggcag taactgccaa tccccgcgcg cccagcgatg ctgacgcgcc
    10441 gccgaagcct cgtagtggga tggaaagtcg tcaaaaagct cgatatcgct aacgagggcg
    10501 gcgcgagcaa agattccctc aaagagatcg tggctcagga ctacgttctc ccgaaccttc
    10561 ccggccaggg cggcttcgaa ggcatcaatg tcgtaaattc ctttacccgt gtacgagcct
    10621 tcgtgaaata gatcctgata tacgtcggag acggcggacg agtacgggtc gattccactg
    10681 ggcccggaaa atactcgctg gaaaatcgag ccacggttgt ccatcggcaa agaaggagta
    10741 actcttggct ggatgattcc gtagcctgcc actacgcggc tctgttcgtt gctaaacttg
    10801 ggccgattga gagggtgggc catagcgccg gcaagccgcc cagctgtgcc acgtggcaaa
    10861 cgggtatcag catccaaagt gatgacgtag cgaacatcgg ggatcgattc gatggcgtgt
    10921 ccggctagag gaatgaaagt cgtgtttgtc gatccacgaa gcagcagatt tagctcgtga
    10981 agtttcccgc gcttgcgttc ccatcccatc cacttctgtt ccgattcatt ccagacgcgc
    11041 tttctatgga atataaaaaa gcggttacca ccgtctttag ccggaccgta gcgcttgttg
    11101 agattcgcga tgccctcggc agcgacggcc aaaagctcct gatcgctggg gagagtttcg
    11161 gtggccgcgt caatccaatc ggaaaggagg ccgaaacgaa gatcgccatc ctgatttgcg
    11221 aggtaatgaa tttcgagttg atgaatctgt tcggcaatcc cgcccgtgga acttagaaga
    11281 gttggtatca cgatgatagt tcgaaggtcc gccggaacgc ctttttcaaa ttcgagacgc
    11341 ggaagggtcc ggggtttgaa caggttggtt acgagcctat tgatcagagc aattgccagc
    11401 tcggaagcgg gaacgacggc gagcacagtt aaaatgataa tttctgggat acgcaggccc
    11461 agctggagcg aacgggccac tggaaaggcc aagataattc cggtaaagag cgcgatcgtg
    11521 ccaaggtatc cgcgcacagc agcgcgcatg taccagcgaa ggaacgcgcg ccttttggac
    11581 acgcggaatc caatttggcg ttcaaaagct gtgcgtccct gcgcgatcag gtagtagccc
    11641 ggatctgaaa acgtctcgtc gtgaggttga tgatcgtgat ctgcgcccgt cgcagccgca
    11701 tctctcgtat gctccacgac gagctccgta atctcgagct cggagagttc cgagcccctg
    11761 gagagatcct cgatggcatg cctgtaagca tcccgcgtgc tgaagtccat ctctccaaac
    11821 ttgctaccct tccgaagggt ctggtccacc aggctgacac tctcgaaaaa agtgtgccaa
    11881 tcgaactccg acgtccaccg catgctggtg atgacgttgc gcaccgtcac gctcatcgcc
    11941 gcctgctgct ggtgttcggc gcggacaata tcgtccgctg ttgtaccttg tgccgcgagc
    12001 cgttcatcga gccacaagag aataggaccg accttcgggt ccaagtcccg caaacgctga
    12061 atcaattcaa ccgcaaaggc ccgatcgagt ggcttctttt cgaactgccg gagggccgcg
    12121 gctggtgata gcgcggactg cccgcctgtg cctaaaagcg cgtcggccag ttggtcggcc
    12181 tggtgacgcg cctggcggct gaatacgatt cggcctgcca gtctgcgcag attttccacc
    12241 agcacaattc gcaaggtgat agcaacggcc cagagctccc caatcgaaag aggctggacg
    12301 cgctgatatg catttatgaa gcgacgaaaa acatgaggat cgagcctgct atcggtgtga
    12361 gctacaaacg cccacgcaat accgtagacc cgcgggtatc cgatcaaatg ccccgaagcc
    12421 aacttaggca atcggcgata gtagccgatt ggcaagtcat cgcgaatctc tcgaagctgg
    12481 tcctcgacga tatagaagtt ttctaccagc cattgttcgg ctggcgtgat gatttgttca
    12541 cgttggagcg cgcgcgcagt cgcgcggtaa tactcgatca gcgaacgcgc atttccctcc
    12601 gtacgctcga tgagagacgg gccgccctct gctgccgcag ccgccaccgt ctgggccgcg
    12661 gcaagactct ccgcgtgctg ctccagccga tcggcgctaa atagctcagc aacgatgggt
    12721 tcttcgattt caacgactac gttcggagca ggtggtgcgg aaaccattgg cttcctcggg
    12781 ctcgcttcca agggctcgcg ctcggctgaa gccgggctgc cctcattgcg aaatagcgcg
    12841 cgctactcgt taacggaagc ttctttacat cctatcgggc caggaaaacc ttaccagagg
    12901 cggggagaga acggcggaca ttttctgagt caacaatcaa acttcatttt tccgagttgt
    12961 gagcatcaca cggctcgcag cttaccggaa ccggcttcga gggttgaata agtgtcccgc
    13021 gagcgcatgc tggcgtgctg cgtcgagatg gcggaatgta gtattcgttc tagatcggcc
    13081 ttgcgatagg gatagcagat atagtcaaac gctcgcatat ttagagctgc aaggtaatcg
    13141 cggtattctt gagagcaccc gggagcgcat accacaatta caggtatctc cacaggttgg
    13201 cgcctcaact gccgcactac gtctcggtaa gtacc
//
LOCUS       DATNDT010000126        14494 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2037809, whole genome shotgun sequence.
ACCESSION   DATNDT010000126 DATNDT010000000
VERSION     DATNDT010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 14494)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14494)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14494
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2037809"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(48..1082)
                     /gene="tdh"
                     /locus_tag="VN788_03990"
     CDS             complement(48..1082)
                     /gene="tdh"
                     /locus_tag="VN788_03990"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HXU19726.1"
                     /translation="MKALVKSNAKPGLWLEDVPVPEFGINDVLIRVLMTGICGTDLHI
                     YEWDEWARSTITVPLVIGHEFVGEITEVGSNVVDLRAGDIVSGEGHVVCGRCRNCLAG
                     RRHLCAHTMGVGVNRPGAFAEYIVLPMSNIWRHSRKIDLESAAIFDPFGNAVHTALSF
                     PVLGEDVLITGAGPIGIMAAAVVRHAGARHVVITDIKPKRLQLAEQLGVTLAVNTNEM
                     RLSDVQKKLGMHEGFDVGLEMSGNADAFREMLANMSHGAKIAMLGIPSKETAIDWKQV
                     IFNMLTIKGIYGREMYETWYKMTVMLESGLDIKPVITHRFAWCEYEKGFAAMKSGDSG
                     KVILDWREAR"
     gene            complement(1131..1649)
                     /locus_tag="VN788_03995"
     CDS             complement(1131..1649)
                     /locus_tag="VN788_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HXU19727.1"
                     /translation="MTVIYVVTTGGTIEKAYSEQNGIVANLDSKIDRYLRLLRLPDCD
                     VHIVPLMNIDSLEMKDSDRAMILGTVNALLQEKAPILITHGTDTMVETGLYLQRALPK
                     LEVPIVMTGAMAPLGFEGSDALQNLTESLIALRILSPGIYVVSHNQVFPISNVRKDRV
                     LGRFVRAEENSA"
     gene            complement(1807..2355)
                     /locus_tag="VN788_04000"
     CDS             complement(1807..2355)
                     /locus_tag="VN788_04000"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU19728.1"
                     /translation="MRKATVILLCGIAALFVASAVSSRSTSRTSYAEDRAEIEDLQGR
                     YMFALDFHDADMYASTFTEDGVLDYGPVVKGREAIRKVIADMGKRAPVQAADASDKRP
                     VVGRHNISNIVIKVDGNKAEGRAYWFHYGNNNPKRTASLDSFGHYEDEMVKVDGKWLF
                     SKRKIYNEQVDKWAYKGGNPAW"
     gene            2921..5326
                     /locus_tag="VN788_04005"
     CDS             2921..5326
                     /locus_tag="VN788_04005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HXU19729.1"
                     /translation="MEERFYRATMKIGGGTLFGRLALAFLLLLSIGIGAAIGLLFVYS
                     SDLPEIRALEDYRPVVVTELYADDGQSIGTFSLQRRILVTYNQIPPVLKDAILTTEDQ
                     HFEEHWGVDFPRVLEAAWRDIVSHRVAEGASTLTMQLAGGLFLNRSDRSFHRKIEETI
                     LALQIERHYTKDQIFTMYCNQIYLGSGNYGFEAASEYYFSKPIGQLNLPEAAMLAAII
                     RGPIYSPVNHPKRALARRNLVLSLMAHDGKITSQQADAASRLPLGLHIQSQRNDLAPY
                     FVEDIRQYLEQTYGTNAVHEQGLRVYTTLNVAMQKAADQALRDGLHSYDRRHGWRGNL
                     PNILRDHLGTLTSYQNPDWHGEIQKGAYVTGLVTAAQPTFAWIKIGPYRAILAPADYS
                     WTGHKVATDLLKVGDLVTVQIKEITGTAAQVELEQIPAAQAALVAIDNSTGEIKAMVG
                     GYDFETSKFNRATQAFRQTGSSFKVYVYSEALAQGASPFDTVVDEPVTFRSGGQNYSP
                     HNYDAKFEGRITLRRALADSRNIPAVRLLDKVGIQNVVSLARRFGITSPLPPYLPLAL
                     GAADLTLLEHTSAFTVFPDDGIHIDPHDIRRVLSYDGAILEEPRAKITDVLEPQVAQT
                     MTAMLEDVVQFGTGVRAKALGRPSGGKTGTTNDFTDGWYIGFTPQITAGVWVGNDDKR
                     VSLGKKETGAQVALPIWLEFMQQAMQGMPIEKFPNVTPLEKLAPTQQVQVDTPDTAPP
                     ADAAEQGLTQVSAPAPTPPKPESAAKPEPAKPAPPKPESAKPSPPLPVPVAVERSKPT
                     SPQ"
     gene            complement(5583..6611)
                     /gene="thiL"
                     /locus_tag="VN788_04010"
     CDS             complement(5583..6611)
                     /gene="thiL"
                     /locus_tag="VN788_04010"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HXU19730.1"
                     /translation="MEQEAQLIERVRRAIPSRESGGLRLGIGDDAAVIAARDGSEWVL
                     SCDAFLEGVHFFADRHPADSVGFKALARAASDLAAMGAQPRIFLLTLALPGSRTGKWL
                     DEFLAGMGRAARKLGMTIAGGDLTSNLKVSISITVVGEVARGLAIKRVGARPGDILCV
                     SGRLGGAKLGLELIKSGALTTANLKQKMYRKPLQAHLYPQIRVGLGAWLATRRIASAM
                     IDISDGLSTDLARLCSASRVGARVWADRIPCVEPPSGKTGRAVSKLLAKLKSDPLRMA
                     LEGGDDYELLFTVPRRKVVRLRHAPGFSEIASVGEIERGREITLVGNGGRPRRLHPGG
                     WDPFERKR"
     gene            6890..7807
                     /locus_tag="VN788_04015"
     CDS             6890..7807
                     /locus_tag="VN788_04015"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HXU19731.1"
                     /translation="MKGKSYTFFIASHDSGGIKRVRVPVYLLHAVAILAVVGTVTVAV
                     AMSSYSRMLWKATNYNSLRRDQDSLKKQYVQLQTQVKDTNTRLNSLQSLAGEVAMAYG
                     ITRFHQTAFDLSDASTRPDVEFQESVDEFSYLAKNVTAVSMSNGGMRLLPASQLSNLG
                     IVPTLWPVLGQITGHFGERLDPFSGEGAFHAGLDIASRYGDAIHVTADGVIEEAGQRA
                     GYGRVVVVDHGFGVTTLYGHLSAFHVQAGMRVKAGDVIGYEGDSGRSTGPHLHYEVRI
                     YNTPVNPWRYLRNVAPVVKAESTPVTTGD"
     gene            complement(8045..8557)
                     /locus_tag="VN788_04020"
     CDS             complement(8045..8557)
                     /locus_tag="VN788_04020"
                     /inference="COORDINATES: protein motif:HMM:NF018942.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030416 - methylamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MauE/DoxX family redox-associated membrane
                     protein"
                     /protein_id="HXU19732.1"
                     /translation="MKLGKILLLVGRIALGAIFLIAAYGKLKPQYAMPWSAHSIKMSV
                     SLFAMQVDSYQMLPPHAVNFVAHVLPFFEVFLGLWLISGIALRVSSIVATLAFCGFMV
                     AIFSAYHRGLGINCGCGIGPVEQVGPGALIRDGLKFLPLSLAVVIGSFWLHRKSRPAP
                     SPETAASIQA"
     gene            complement(8951..9712)
                     /locus_tag="VN788_04025"
     CDS             complement(8951..9712)
                     /locus_tag="VN788_04025"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXU19733.1"
                     /translation="MSYASKDSSGANASKKPGNRLGAWCALAATIVLGLSFASLAVGQ
                     GAHARTVSAKGARSKSVEAPSAPLKSYGSSSAPITMEVFTDYECPSCRSLYENTLRPM
                     INDYVASGKVYLVHHDFPLPGHKYGFEAARWLNAAARVGQFQNAEAALYDNQDAWAAD
                     GNIEKFIAAAMPASEFKRVQKLMVGCEYEPPGTAGQSCALDNYINADRELGMKVPVQA
                     TPTYVITYKGQKLPAGSGAVSWPILKQFFDSLLSK"
     gene            10044..11993
                     /gene="dnaK"
                     /locus_tag="VN788_04030"
     CDS             10044..11993
                     /gene="dnaK"
                     /locus_tag="VN788_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HXU19734.1"
                     /translation="MSKIIGIDLGTTNSAVAVMQGGEPVVVPNQEGARTTPSVVAITK
                     SGERLVGQVAKRQAITNPENTIYSIKRFMGRRYDEVNEEMKMVPYKVVRQGDHVAVTA
                     QGKEYSPPEISAMILQKLKTAAEDFLGEKVTEAVITVPAYFNDAQRIATKDAGKIAGL
                     DVKRIINEPTAAALAYGLDKKKDETIAVYDFGGGTFDISILEVGEGVIEVKATNGDTH
                     LGGDNIDQRIVDWLIEEFKKDEGLDLRGKGNEMALQRLRDAAERAKIELSTTMETEIN
                     LPFVTADASGPKHLVKKLTRGRLEAMVDDIIRRSVEPCKLCMKDAGVDPGKINEVVLV
                     GGQTRMPKIQELVKGLFGREGHKGVNPDEVVAVGAAIQGGVLGGEVKDILLLDVTPLS
                     LGVETLGGVTTVLIPRNTTIPTRKSETFSTAADNQTSVEIHVLQGERPVASGNRSLGK
                     FHLIGIPPAPRGVPQVEVTFDIDANGILNVSAKDNATNKEQKITITASTGLSKEEAER
                     MKKEAESHAAEDKARLSEVEARNRLDNLVYQTEKLTKENREKLDEADAKAAEEAIEEA
                     RRALTDGGLEKLNAAFDGLTKASHRIAESLYKSQQAAAGSSGPGGGAGQPGPEGSTNA
                     NGNPPGQGDVVDAEFVDVDESKKPN"
     gene            12139..13311
                     /gene="dnaJ"
                     /locus_tag="VN788_04035"
     CDS             12139..13311
                     /gene="dnaJ"
                     /locus_tag="VN788_04035"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXU19735.1"
                     /translation="MATATKQDYYELLGVSRKAPVKEIRAAYRKLARKYHPDLNPGDK
                     TAEEKFKQIQEAYEVLSDTKKRQMYDQFGFNTPGMGGGAPGPGAGYGGAAPQDIHFDF
                     GGFDFGGGGAEGAAGGASFRDLFSQFFRGASAAQAGREREAGSDLEYQIDITFAEAMR
                     GTVKKLSFTRLDVCNVCHGQGYQGEEKVCPTCGGSGHVTQVSGKMRFQVGCSRCGGSG
                     HLRTICRNCGGEGRVSRVDTLDVRIPPGARTGSRVRVAGRGNAGTNGAPPGDLYIVMN
                     VQPHPFFERRGDDLYTVIPVTVTEASLGAKVEVPTIDGRSQVRIPPGTSSGKKLRLRE
                     KGAPSMRQTGKRGDQIMEVQVVVPKPEDERVRNLLKELSKLDPEDPRQEIFSRATV"
     gene            13341..13739
                     /locus_tag="VN788_04040"
     CDS             13341..13739
                     /locus_tag="VN788_04040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020259293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU19736.1"
                     /translation="MAVRPRKKAKAGYMISAVAELYQLHPQTLRLYERVGLLKPSRSQ
                     GNTRLYTDGDLERLEVILNLTREMGVNLAGIEIILNMRDKMSEMQAQMEAFTKFVRNE
                     VARNLGIPDESRSRNAIVRAAPRHAIRVQK"
     gene            13892..14203
                     /locus_tag="VN788_04045"
     CDS             13892..14203
                     /locus_tag="VN788_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019018950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXU19737.1"
                     /translation="MYDMKNLSKLKLMDASAPDGMKAFWAFDKAAMAAGAIPVKYKEL
                     LALAVAFTTQCPYCIEIHSGRARDAGASNEEISETVVVAAALRAGAAITHGTHALKAG
                     E"
     gene            14242..>14494
                     /locus_tag="VN788_04050"
     CDS             14242..>14494
                     /locus_tag="VN788_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19738.1"
                     /translation="MRRLALAGWAAYACEMARADCPLCQGSGWKVVERTTEGAQALAT
                     KESARPGSASGEPKMVWAVPCDCTSGDRTGRVLAKARVPE"
BASE COUNT         3207 a         4184 c         4148 g         2955 t
ORIGIN      
        1 tcatattgtg ttcttgcggc ttcgaacata tgcgtgcgct cccactctca gcgcgcttcg
       61 cgccaatcca ggatgacctt gccggaatcg cccgacttca tggccgcgaa tcctttttca
      121 tattcgcacc atgcgaaccg atgcgtaatc acaggcttaa tgtcgagtcc cgattccagc
      181 atcaccgtca ttttgtacca ggtctcgtac atttcgcggc cataaatgcc cttgatcgtc
      241 agcatattaa aaataacttg cttccaatcg atcgctgttt ccttcgacgg aatgccgagc
      301 atcgcaattt tcgcgccatg gctcatgttc gcgagcattt cacggaacgc atccgcgttt
      361 cccgacattt ccagtccaac gtcaaaccct tcgtgcattc caagtttttt ttgcacatcc
      421 gataagcgca tttcattcgt gttcactgcc aacgtcacgc cgagctgttc ggccagctga
      481 agacgcttcg gtttaatatc ggtaatgacc acgtgacgcg ccccggcgtg gcgcaccacg
      541 gcagcagcca taattccgat tggccctgcc cctgtgatca atacatcttc gcccaaaact
      601 gggaacgaca gcgcagtgtg caccgcgttc ccgaacggat cgaagatggc cgcgctttca
      661 aggtcgatct tacggctgtg cctccaaata ttgctcatcg gcagcacaat atattccgcg
      721 aacgcgccgg gacggttcac tcccacgccc atggtgtgcg cgcagagatg ccgccgtccg
      781 gccaggcagt tgcggcaacg tccgcaaacg acatggccct cgccactcac gatatcgccc
      841 gcccgcagat cgacgacgtt cgaccccacc tcggttattt ccccaacaaa ttcgtgcccg
      901 atcaccaatg gcacagtaat cgtcgaccgc gcccactcat cccattcgta gatatgcagg
      961 tccgttccgc aaataccggt catcagcacg cgaatcaaaa cgtcattgat gccaaattcc
     1021 ggaacgggca catcttcgag ccatagccct ggtttggcgt tgctttttac cagcgctttc
     1081 atattcaccc tcgcttctca cattaacacc aagggaaggc catgcggtcg ctacgcggag
     1141 ttctcttcag ccctcacaaa tcgcccgagc acgcggtcct tgcgaacgtt tgagatggga
     1201 aacacctggt tatgactcac aacataaatt ccaggtgaaa ggatgcgcag cgcgataagg
     1261 ctctctgtca gattctgcaa agcgtcgctg ccttcaaacc cgagcggcgc catcgctcct
     1321 gtcatcacaa tcggaacctc taacttcggc agcgcgcgct gcaaatatag tcctgtttca
     1381 accatcgtgt ccgttccatg cgttatcaga ataggagcct tctcctgaag aagagcattc
     1441 acggttccga ggatcatggc gcggtccgaa tccttcattt ctaaactgtc gatgttcatc
     1501 aacggcacga tgtgcacatc gcaatcagga agtcttagta gccgcaggta gcggtcgatc
     1561 ttgctgtcga gattcgccac aatcccgttc tgttcggaat aagctttctc gatcgtcccg
     1621 cccgtggtca ccacatagat caccgtcatc gccccggtcc ccccttttaa caatccagcc
     1681 ggcgagtgaa agcaaatcag ggatctagcc gccgcgctag cctcgcggct ctgaagcaac
     1741 aacttgaatt ttgaatttgg tcctcgtcgg ttttgcgagc gttggccgct ggctcggcgc
     1801 gagttcctac caggccggat ttccgccttt gtaggcccac ttatccacct gctcgttgta
     1861 aatctttcgt ttgctgaata gccacttgcc gtccactttc accatttcat cctcgtaatg
     1921 gccaaacgaa tcgagactgg cagttcgttt gggattgtta tttccgtagt gaaaccaata
     1981 ggctcttcct tctgccttgt tcccatcgac ttttatgacg atgttcgaaa tattgtggcg
     2041 tcccacgacg gggcgcttat cggaagcatc ggccgcttga actggcgcgc gtttgcccat
     2101 atccgcgatc acttttcgaa tcgcttcgcg gcctttcacc acaggaccgt aatcgagcac
     2161 cccatcctct gtgaacgttg atgcgtacat atcggcatcg tgaaaatcca gcgcgaacat
     2221 gtaccgtccc tgaagatcct cgatctcagc ccgatcctcg gcgtagctgg ttcgactagt
     2281 cgatcgtgaa gaaacagcgg acgccacgaa cagagccgca ataccacaca acaaaataac
     2341 tgtcgctttc cgcatttccg ttcccctcct gtttggattc tctggtccgg cggattccgg
     2401 tgcaccggat aatacaaaaa gcatctcccc ccggctattc gttttagacg aatctctctt
     2461 cgatgtaagc cgcggcaaca tttcacctcc agcgagtcgc ccaggccgtc aagcgtgcgt
     2521 ttttgaattt gcggcgggtg ccccatgctc gttttgtgag cgtggggttt ttcgcttccc
     2581 gctcacgaga ttgtgctctc gcaattgtcg cttcctcgcc gtgaggcctt tggcgattga
     2641 accctcaaac tcatcgcctc acgctctggg tgtcattccg agcgaagtgt cccgcccttt
     2701 tgcctttgcg cgaagcgccg ggacacgcga ggaagggctc ccgggtcgac ggttagtcga
     2761 gcccgggaga tcagacgaat ctctcttcga tctaacccgc ggcaacattc acttccaggc
     2821 gatcgacagc cgctctagcc aacgaatcgt gtctcgtccg ccagaaacca attcgtcaac
     2881 cagcccgact caagaccccc aatgcgatag aatatcccca atggaagagc gattttaccg
     2941 ggccacaatg aagattggcg gcggcacttt gttcggccgc ctagccctcg cgttcctgtt
     3001 gctgctttcc atcggaatcg gcgcagccat cgggctgctc tttgtgtact caagcgacct
     3061 gccggaaatt cgcgccctcg aagattaccg ccctgtcgta gttactgagc tttatgccga
     3121 cgatggccaa tccataggaa cgttctctct tcagcgccgc attctcgtca cttacaacca
     3181 aattccgcca gttctaaagg atgccatcct caccacggaa gatcagcatt tcgaggagca
     3241 ttggggtgtg gacttcccgc gcgtgctcga agcggcctgg cgcgatatcg tttctcaccg
     3301 tgttgccgaa ggcgccagca cccttaccat gcagctcgcg ggcggtcttt ttctgaatcg
     3361 ctcggaccgc agcttccacc gcaagatcga ggaaaccatt ctcgctcttc aaatcgagcg
     3421 ccactacacc aaagaccaga ttttcaccat gtactgcaat cagatttacc tgggctccgg
     3481 aaactacggc ttcgaggccg cctcggaata ttatttcagc aagcccatcg gccaattgaa
     3541 ccttcccgaa gccgcgatgc tggccgccat catccgagga cctatctatt ccccagtcaa
     3601 tcatccgaag cgggctctgg cgcgccgcaa tctggtcctc tcactcatgg cccacgacgg
     3661 caaaatcacc tcgcaacaag ccgacgccgc aagccggttg cccctgggac tccacatcca
     3721 atcgcagcga aatgatctcg cgccctactt tgtcgaggat attcgtcaat atctcgagca
     3781 gacttacggc accaacgccg ttcacgagca aggattgcgc gtttacacca ctctaaatgt
     3841 agcgatgcag aaagctgctg accaggctct tcgcgacggc ttgcactcct acgatcgccg
     3901 tcacggctgg cgcggtaatc ttcccaatat tcttcgcgat catctgggga cactgacaag
     3961 ctatcaaaac cccgactggc acggcgagat ccaaaagggc gcctacgtca ctggtctggt
     4021 caccgccgcc caaccaactt tcgcctggat caagatcggg ccctatcgcg ccatcctcgc
     4081 tccagccgat tattcctgga cgggccataa agtggctacc gacctactaa aggtcggcga
     4141 ccttgtcact gtgcagatca aagagatcac cggcaccgcc gcgcaagtcg aactcgaaca
     4201 aattcccgcc gcgcaggccg ctctagtcgc catagacaac tccacgggcg agatcaaagc
     4261 catggtcggc ggctacgatt ttgaaacctc gaagttcaat cgcgccacgc aggcattccg
     4321 tcagacgggc agttccttca aggtctatgt ctactctgag gcgctggcgc aaggagcctc
     4381 gcccttcgac accgttgtgg acgagccagt cactttccgc agcggcggcc aaaattattc
     4441 gccgcataac tacgacgcca aattcgaggg ccgcatcacc ttgcgccgcg ccctcgccga
     4501 ttcgcgaaac attccggccg tgcgcttgct cgataaagtg ggcatccaaa atgtcgttag
     4561 tttggcgcgg cgctttggca tcaccagtcc tctgccgccg tatcttccgc tcgctctcgg
     4621 cgccgcggac ttgacgctcc tcgagcacac ttccgcgttc acggtttttc ccgacgatgg
     4681 aattcacatc gatccgcacg atatcaggcg cgttctttct tatgacggag ccattctcga
     4741 agagccacgt gcgaagatca ccgacgtgct cgagccgcag gtggctcaaa ccatgacggc
     4801 gatgttagag gatgtcgttc agttcggcac tggcgttcgt gccaaggcgc tcggccggcc
     4861 ctccggcgga aagactggca ccaccaacga tttcaccgat ggctggtaca tcggtttcac
     4921 tccgcagatc accgcgggag tttgggtcgg caacgacgat aaacgcgtct ccctcggcaa
     4981 aaaagaaacc ggcgctcagg tcgctcttcc catctggctc gagttcatgc aacaagctat
     5041 gcagggaatg ccaatcgaga aattccccaa cgtgacgccg ctcgaaaaac tagcgcccac
     5101 gcagcaggtt caagtggata caccggacac agctccgccg gccgatgccg ccgagcaagg
     5161 cctcacccaa gtttccgccc cggcgcccac tccgccaaag cccgagtccg cagcaaagcc
     5221 cgagcccgcg aaaccggcgc cacccaaacc tgaatcggca aaaccgtcac caccgctacc
     5281 ggttccagtg gccgttgaaa gatcgaagcc aacttctcct caataaattt caatctccag
     5341 acgaaatcgc gccgccgcag caaaagcttc ggccttgcct tgtgtggcgc catccctttt
     5401 agggtggcat cccgcttttg ctcggccttt gaatttcgtc gcagcgcaca ccttgatttt
     5461 taggcgggcg ccgtgtcttg tgtagtgcca tcccctttta ccctgacgaa ggaagggcag
     5521 ggtggcatcc cgcttttgcc tgcttctctg aactaagcgc gacgtgaaaa gaaaacgtat
     5581 cgttaccttt tccgttcgaa gggatcccaa ccgcccggat gcaggcgcct cggccgcccg
     5641 ccgttaccaa cgagcgtgat ttctcttccg cgctcgattt ctccaacgga cgcgatctca
     5701 gaaaatcccg gcgcgtgccg aagccgcacc accttccgtc gagggacggt gaaaagcaac
     5761 tcgtagtcgt cgccgccctc gagggccatt cgcagcgggt ccgattttaa ctttgccaaa
     5821 agtttggata cggcccggcc tgtttttccg ctcggcggct cgacgcaagg aattcggtcg
     5881 gcccaaaccc gcgcacctac ccggctcgca gagcacaatc gcgccaagtc ggtcgaaagt
     5941 ccgtccgaaa tgtcgatcat cgcagacgcg atccgccggg tcgcgagcca ggctcccaag
     6001 ccgacgcgaa tttgcggata aaggtgcgct tgcagcggtt tgcggtacat tttttgtttc
     6061 aagtttgctg ttgttaatgc gccagacttg attaattcca agcccaattt ggcgcctccc
     6121 agccttccac tcacgcacaa aatatcgccg ggccgcgccc caacccgttt aattgcaagc
     6181 ccgcgcgcca cctctcccac taccgtaatg ctgatcgaaa ccttcaagtt tgaagttaaa
     6241 tctcctccag caatggtcat accgagtttt cgcgccgcgc ggcccattcc ggccaaaaat
     6301 tcgtcgagcc attttcctgt tcgcgagcct ggcaacgcca gcgtcagcaa aaagatgcgc
     6361 ggctgcgcgc ccatcgccgc gagatcgcta gcggcgcgcg ccagcgcttt aaagccaacc
     6421 gaatccgccg gatgccggtc agcaaaaaag tgaacgcctt ccagaaacgc gtcgcagctc
     6481 agcacccact cggagccatc gcgtgcagcg atgaccgcag cgtcgtcgcc aattcccaga
     6541 cgcaagccgc cgctctcgcg tgaagggatt gcgcgcctaa ccctttcgat cagttgagct
     6601 tcctgttcca ttcctgtcca tctgtacagt tgtaaccctc agcccgtttc gggtagagtt
     6661 tcagaagggg gcactggaat tataaagtgt cgccaggacc gacgcgagcc cagcgctgcg
     6721 ggtggaagat cggcattgac gccgatggtc gggctgacta caatccgcgc gggtttcgaa
     6781 ccagggctct cggttcagac tctcaatcga agttgcacgc gaatcgctaa ccgcaatctc
     6841 aggcagcggt ttcaggccgg cgaaaaatca aacgggtgaa gctaagagca tgaaaggcaa
     6901 gtcgtacacg tttttcattg cgtctcacga ctcgggcggt attaagcgag ttcgagtacc
     6961 cgtgtatctt ctgcatgcag tagcaattct cgcggttgtc ggcaccgtca ccgtggccgt
     7021 cgcgatgagc tcctactcgc gaatgttgtg gaaagcgacg aactacaatt cgcttcgacg
     7081 cgatcaggac agcctcaaga agcagtacgt ccagcttcaa actcaggtga aggataccaa
     7141 cacgcgcttg aattctctgc aatcattggc gggcgaagtg gcgatggcct acgggatcac
     7201 gcgcttccat caaaccgctt tcgatctcag cgatgcttcg accagacctg acgttgaatt
     7261 ccaagaatcg gtggatgagt tcagctatct cgcgaaaaac gtgactgcgg tttcgatgtc
     7321 gaatggcggg atgcggctcc tgcctgcatc gcagctgagc aacttgggaa ttgtgccgac
     7381 gctttggccg gtgctgggac aaatcaccgg gcatttcggc gaacggctgg atccattcag
     7441 cggtgaaggc gcgttccacg ctggtctgga tatcgcttca cgctacggcg atgcaatcca
     7501 cgtaactgcc gacggcgtga tcgaagaagc gggtcagagg gcgggatacg gccgcgtggt
     7561 tgtggtggat catggttttg gagtgaccac gttgtatggc cacctttctg ctttccatgt
     7621 gcaagctgga atgcgcgtga aagctggaga tgtaattggg tacgaaggcg atagcggccg
     7681 ttcgacgggg ccgcatcttc actacgaagt ccgcatctac aacacccctg tgaatccctg
     7741 gcggtatctc cgcaacgtcg cgccagtagt aaaagccgaa tccactcccg tcaccacggg
     7801 cgactaagct tttccagcga cttatcgcgc tttgctcatc gtgtaaaata tttcggtgtg
     7861 gcaccaaagg aggccgtcat ggccctatct gatgaagaat tcgaacgcct aagcaacagt
     7921 cgcgccagcc acagaatctt gtggagttct ttgcggaatc tccgctggcc gaagtcgtga
     7981 tcgatttgga gcgcgagccc gactacggcc gggtagtcga actctgagcg gctttctttg
     8041 cgaattaggc ttggatggaa gctgcggttt cgggagatgg ggcggggcgg gatttgcgat
     8101 ggagccagaa ggagccgatt actacggcga gagagagcgg gaggaacttc aggccgtcgc
     8161 ggatcagggc accggggcct acttgttcga cggggcctat tccacagccg cagttgatgc
     8221 ctaagccgcg atggtaggcg gagaagatcg cgaccatgaa tccgcagaac gccaaggttg
     8281 cgactatgga tgagacgcgc aaggcgatgc cgctgatcag ccaaagtccc agaaacactt
     8341 cgaaaaatgg caggacgtgg gcgacgaaat ttacggcgtg cggcggaagc atttgatacg
     8401 aatccacctg catcgcaaac agagaaacgg acattttaat tgagtgtgca gaccaaggca
     8461 ttgcgtactg cggcttcaat ttcccgtaag cggcgatcag gaaaatggcg cccagggcga
     8521 tgcgtcctac gagcagcagg atttttccaa gtttcatgcg cgcgtctgag ttcatgggaa
     8581 ttttaagttc atcagttccg cagctaaagt tcttacaccg ctgctttata tccgccgcaa
     8641 gctcgtcggc aacgcgagcg ccaatgaaga catgctgcgg gtgaagctgc gcagtggaaa
     8701 gcgctgcgcg ctgtcttctt atggaagggc tgaagccccg cccttccacc gatgtcctag
     8761 gcgctaaagc ggcatcggcg gcgatcttcg tgtcggagtt gaagctccgt ccccaaagga
     8821 agcgcttggc gcgtcagttt atggcgctga agtcccgacc cactaggcat cgaatttcct
     8881 tcgggccggt aagtcgcgcc tgacgcagcg tctaagctca tcgtttttgt cttgtgggcc
     8941 gaccccttta ctacttactc agaagggaat cgaagaactg cttcaagatg ggccaggaaa
     9001 cggcgccgga acctgccgga agcttttgtc ccttgtaagt gatcacgtat gttggcgtag
     9061 cttgcaccgg aaccttcatc cccagctcgc ggtcggcgtt gatgtagtta tcgagggcgc
     9121 aggactggcc cgcggtgccg ggaggctcat attcgcaccc gaccattagc ttttgaactc
     9181 gcttgaactc cgatgccggc atggcggctg cgataaattt ttcgatgttg ccgtcggccg
     9241 cccacgcgtc ctgattgtcg tacaaagccg cttcggcatt ctggaattgg cccacgcggg
     9301 ctgcggcgtt cagccagcgc gccgcttcga atccgtactt gtgcccgggc agagggaaat
     9361 cgtgatgaac cagataaact tttccgctgg cgacgtagtc gttgatcatg ggacggagag
     9421 tattttcgta aagactgcgg caggatgggc attcgtaatc ggtgaagact tccatggtga
     9481 tgggggcgct gctcgacccg tagcttttta gcggcgcact gggagcctcg acgcttttcg
     9541 aacgagcgcc tttagcggag acagttctcg catgcgcgcc ctggccgacg gccagagatg
     9601 caaacgagag accaaggacg atggtcgccg ctaaggcgca ccatgccccg aggcggttac
     9661 ccggtttttt cgacgcattc gcgcccgatg aatcctttga tgcgtagctc aaatatgcct
     9721 ccagaaatcg aaataaactt gattgtccgc attgtagcgc gaatcgccgc ccgagcatcc
     9781 tacggttagc agcatgaagg cgattttgat accgctgaat ggttaggatg cgtcggccgt
     9841 ggagttggtt cgctgggagc gaaagtagga ggcggacggg aagagtcaag ggaaggaggg
     9901 tttattgcgg gggaagtggc gcggtatcag cgtgatacag gcacacaaca atcttgacaa
     9961 tgttacgctc aacgtgtaat ataaaccgtg ttttgaggct aatttagtcc gaccgaggcc
    10021 aggcccggtc aggagttgat tccatgagca aaattatagg tattgacctc ggtaccacca
    10081 actcggcagt agcggtgatg cagggaggcg agccggtggt ggtccccaac caggaaggcg
    10141 cgcgaaccac gccctcggtg gtggcgatta cgaagagcgg cgagcggttg gttggccagg
    10201 ttgccaagcg gcaggcgatt acgaatccgg aaaacactat ttactcgatc aagcggttca
    10261 tgggacgccg ctacgatgaa gtgaacgagg aaatgaagat ggtcccctac aaagtggtgc
    10321 gccaaggcga ccatgtggcc gtgacggcgc aggggaagga gtacagcccg ccagaaatct
    10381 cggcgatgat tctgcaaaaa ttgaagacgg ccgcggaaga tttcctgggc gaaaaagtaa
    10441 cggaagcggt gatcaccgtt cccgcttatt tcaacgacgc gcagcgaatc gcgacgaaag
    10501 atgccgggaa aattgcgggt ctcgatgtga aacgaatcat caacgagcct actgcggcag
    10561 cactggccta cggcctcgat aaaaagaaag atgaaacgat cgccgtgtac gactttggcg
    10621 gcgggacttt cgatatttcc attctggaag ttggcgaagg cgtgatcgaa gtgaaagcca
    10681 ccaacggcga cacgcatttg ggcggcgaca acatcgatca acgcatcgtc gattggctga
    10741 tcgaggagtt caagaaggat gaagggctcg atctgcgcgg aaaaggcaac gagatggcgc
    10801 tgcagcggct gcgcgatgcg gccgaacgcg ccaagatcga actttccaca accatggaaa
    10861 cggaaatcaa ccttccgttc gttacggcgg atgcctccgg tccaaagcac ttggtgaaga
    10921 agctgacgcg gggacgcctc gaggcgatgg tggacgacat cattcgccgt tcggtggaac
    10981 cttgcaaact gtgcatgaag gacgcgggcg tcgatccagg aaaaatcaat gaagtggttc
    11041 tggtcggcgg gcagacccgc atgcccaaaa ttcaagagct tgtgaaagga ctttttggac
    11101 gcgaagggca caagggcgtg aacccagacg aagtggtggc ggtgggcgcg gcgattcagg
    11161 gcggcgtgct gggcggcgaa gtgaaagaca ttttgctgct cgacgtgacg cctctctcgc
    11221 tgggcgttga aacgctgggc ggagtgacga ctgtcctgat ccctcgcaac acgacaattc
    11281 caacgcggaa gtccgagaca ttttcgaccg ccgcggataa tcaaacgtca gttgaaattc
    11341 acgtgctgca aggggaacgt cccgtggcga gcggaaatcg atcgcttgga aaattccact
    11401 tgatcggaat tccaccggcg ccgcgcggcg tgccgcaagt tgaagtgacg ttcgacatcg
    11461 acgcgaacgg cattctgaac gtttcggcga aggacaacgc gacgaacaag gagcaaaaaa
    11521 tcaccatcac cgcgtccacc ggtctttcca aggaagaggc cgagcgcatg aagaaggaag
    11581 cggaatcgca tgcggccgag gacaaggcgc ggctttcgga ggtggaggcg cgcaaccggc
    11641 tcgacaacct tgtttaccag acagagaagc tgaccaagga aaaccgcgag aagttggacg
    11701 aggcagacgc gaaggcggcc gaggaagcga tcgaggaagc gcggcgcgct cttacggatg
    11761 gcggcctcga gaagctgaat gcggcttttg acgggttgac caaggcgtcg catcgcatcg
    11821 ctgaatcgct gtacaagagt cagcaggcgg ctgcgggctc ttcgggaccc ggaggcggcg
    11881 caggacagcc ggggccggaa ggctcgacga acgcgaacgg caatccgccg ggacaaggcg
    11941 acgtggtgga tgccgagttc gtggacgtcg acgaatcaaa gaagccaaat tgaaaaacgc
    12001 gcagggcgtt ttcgccatgc tggcgcgcga cgcgatccgg caggattcat gtgatagggg
    12061 cgtctgcaac cgcaggcgcc cttacgcacg tatggggata ttgcgggcgc ggcggacgct
    12121 ttatttcgaa tagaaaaaat ggccacagca accaaacaag attattacga gctgctggga
    12181 gtttcgcgga aggctccggt gaaggaaatt cgcgcggcgt atcgcaagct ggcgcggaaa
    12241 tatcatcccg atctcaatcc cggtgacaaa acggcggaag aaaagttcaa gcaaatccag
    12301 gaagcgtacg aggtgctttc ggacaccaag aagcgccaga tgtacgacca gtttgggttc
    12361 aatacgccgg ggatgggggg tggcgctccg gggccgggag cgggctacgg cggcgcggct
    12421 cctcaggata tccacttcga tttcggagga ttcgatttcg gcggcggggg cgcggaaggc
    12481 gccgcgggcg gagccagttt tcgcgacctt ttcagccagt tttttcgcgg agcgagtgcc
    12541 gcccaggccg gacgggaacg cgaggcgggc agcgatctgg aatatcagat cgacattacg
    12601 tttgccgaag ccatgcgcgg aacggtgaag aagctgagct ttacgcggct ggatgtgtgc
    12661 aatgtttgcc acggccaggg atatcagggc gaggagaaag tttgtccgac gtgcggggga
    12721 tcggggcacg ttacgcaagt gagtgggaaa atgcgttttc aagtggggtg ttcacggtgc
    12781 ggcggcagcg gacacctgcg cacgatttgc cgcaactgtg gcggcgaggg gcgcgtctcg
    12841 cgcgtggaca ccttggatgt gcgcattcct ccgggcgcgc ggacgggatc tcgcgtgcgc
    12901 gtggccgggc gcggcaatgc tggcacaaac ggagcgccgc cgggcgatct ctacattgtg
    12961 atgaatgtgc aaccgcaccc atttttcgag aggcgcggcg acgatttgta cacggtgatt
    13021 ccggtgactg tgaccgaggc ttcgctggga gcgaaagtgg aagtgccgac gatagacggg
    13081 cgttcgcagg tgcgcattcc gccgggaaca agcagcggga aaaagctgcg gctgcgcgag
    13141 aaaggggcgc cttcgatgcg gcaaacgggc aagcgcggcg accagatcat ggaagtgcaa
    13201 gtggtggtgc ccaagccgga agatgagcgc gtgcggaact tgttgaaaga gctgagcaag
    13261 ctcgatccgg aagatccgcg gcaggaaatt ttctcgcgcg cgactgtttg aggcgcgaac
    13321 ttcagcctca ggagaagaat atggcggtgc ggccgaggaa aaaagcgaaa gcgggataca
    13381 tgatcagcgc agtcgcggaa ctgtatcagc tgcatccgca gactcttcgc ctttacgaac
    13441 gcgtgggcct gctgaaaccg tcacggtcgc aaggtaacac gcggctttat accgacggag
    13501 acctggagcg gctggaagtg atcctcaact tgacgcgcga gatgggcgtg aatctggcgg
    13561 gcatcgaaat tatcctaaac atgcgcgaca aaatgagcga gatgcaggcg cagatggaag
    13621 cgttcacaaa attcgttagg aatgaagtgg cgcgcaatct ggggattccg gacgagagca
    13681 ggtcgcgcaa cgcaatcgtg cgcgccgctc cgcgccacgc tattcgcgtg caaaagtgac
    13741 cacaagggcg tcgccgttag caaccagccg aggcacgcag aatctccgtt cccacctcga
    13801 gcgagacaag ctctgaattc caaagaagtc gaacctatcc agaactttcg gcatcgaatg
    13861 agaattcaaa accaccaaac ggaggatggc catgtacgac atgaagaatc tcagcaagct
    13921 caagttgatg gacgctagcg ccccggacgg aatgaaggca ttttgggcct tcgacaaagc
    13981 cgccatggct gcgggcgcga tccccgtgaa atacaaagaa ctgctggcgc tggcggtcgc
    14041 gttcaccacg cagtgtccct attgcatcga gattcattca ggaagggcgc gcgacgccgg
    14101 agcttcgaat gaagaaattt ccgaaacagt ggttgtcgcc gcggcgcttc gtgcgggcgc
    14161 ggccatcacc cacggcacgc acgcgctgaa ggccggcgaa tagcgcgtcc gagattccac
    14221 aggccgcgcg gatttgcaca catgcggcgt cttgcgctcg cgggatgggc cgcatatgct
    14281 tgcgaaatgg cgcgagcgga ttgtcccctt tgccagggca gcggttggaa agtagtcgag
    14341 cgaaccacgg aaggcgcgca ggcgctggcg acgaaagaat cggcgcggcc cggttcggct
    14401 agcggcgaac caaaaatggt ttgggcggtc ccgtgcgatt gcacgtcggg agaccgcacg
    14461 gggcgtgtgc tagcgaaggc gcgcgtcccc gaac
//
LOCUS       DATNDT010000127         3091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3505570, whole genome shotgun sequence.
ACCESSION   DATNDT010000127 DATNDT010000000
VERSION     DATNDT010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3091
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3505570"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..493
                     /locus_tag="VN788_04055"
     CDS             8..493
                     /locus_tag="VN788_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19739.1"
                     /translation="MSHENRHLPDRLIRIIETDAEQLTQGTVKQLQTSPRTPSYHQLS
                     YGELHERAYSVYHDLGRWLWEKSDRAIQSWYNDLGEKRCSEGVPLAEVLWALVLTKNR
                     LMDYLGKWGLADSAIELYQQQEFDRMVGHFFDRAVCYAAEGYEHQASIQSSSGTETMA
                     G"
     gene            complement(567..1256)
                     /locus_tag="VN788_04060"
     CDS             complement(567..1256)
                     /locus_tag="VN788_04060"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXU19740.1"
                     /translation="MATIVRSQRASPGRRSSPSGNNRNRNGGSPGEPVYELHATAQLD
                     PRGTPISAYRLITFLAIIWIVTLFVTLTLVLEWRWAHSTDWVSIPLPYVLYINTVILL
                     GSSLTMEFARLSLRDDASEKCARWIRVTLLLGLVFLAGQLVAWRELVSGGLHLASNPG
                     SFFFYLITGAHGLHLLGGIVALALVRRFVSRAAPRVKQQAAVDTIALYWHFMDGLWLY
                     LLALLFIGIQR"
     gene            complement(1439..1927)
                     /locus_tag="VN788_04065"
     CDS             complement(1439..1927)
                     /locus_tag="VN788_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19741.1"
                     /translation="MTESAQTPKASVAQQHHRKRSFFHEHSLSIASIGILFLWIGFYV
                     FSNPSTHWGSFFGNAIADWSGVVVTVIATKYLYEKGSAESKPLPKHTLTPVLEWLRDH
                     SLTIFLLITGIGWVVLFARMNPDAKWGQVVGNIVSEWTQIFGLVLLTTRMIERHSKES
                     RR"
     gene            complement(2168..2773)
                     /locus_tag="VN788_04070"
     CDS             complement(2168..2773)
                     /locus_tag="VN788_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19742.1"
                     /translation="MAVKPTNHSVENAEDVAEINSGVPFGKYFDVGRFKDEFEARSAS
                     DDLARIGIHAIVIQKHRMWASSYQVIAGPYPDPQQMQIASTNLRSHGFKTHSLPRKSR
                     DLTLVSSTAPHGLAETPIEQFVVNWETYSPEVTVNFLKAGDTVAKAQGKWVKRSAPYD
                     YDAIVFKQNGNGSRTLLEIWFHGMSRAVVLPTTSPNHSVTF"
     gene            2820..>3091
                     /locus_tag="VN788_04075"
     CDS             2820..>3091
                     /locus_tag="VN788_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19743.1"
                     /translation="MLPGSGGTGPGRAAAKSINGDAAGDGVNEIAGAIGLGASLAGAG
                     LAGCVAGWIRASSKCGAGTGVESGTEIVARRWIVGREAWDEADVGAG"
BASE COUNT          726 a          801 c          901 g          663 t
ORIGIN      
        1 ataaaacatg tcacatgaaa atcggcatct tccggaccga ttgattagga tcatagaaac
       61 cgacgcggaa cagcttacgc agggtacggt caaacagttg caaaccagcc cacgaacccc
      121 ctcgtatcac cagctttcct atggcgaact gcacgagcgg gcctattcgg tgtatcacga
      181 tctaggtcgc tggctttggg agaagtcgga ccgagcgatt caatcttggt ataacgacct
      241 cggggagaaa agatgcagcg agggcgttcc tttggccgaa gttctgtggg cgctcgttct
      301 aaccaagaac cgcctaatgg actatctcgg caagtggggt ctggcggatt ccgccatcga
      361 actttatcag cagcaggaat tcgataggat ggtcggccac tttttcgatc gggctgtctg
      421 ctatgcggcg gaaggctacg agcatcaggc gtcgattcaa agcagcagcg ggacggaaac
      481 gatggcgggt tgaggagggc ttaagggtct cagctgcgcc aaaggtgcct gttacgcgca
      541 agggacgact tacaatggtc gttgcgctat cgctgaatac cgataaaaag cagcgccaat
      601 aaataaagcc agaggccgtc cataaaatgc cagtaaaggg cgatggtgtc taccgcagct
      661 tgctgcttaa cccgcggtgc tgctcgacta acgaagcgac gcactagagc aagagcgacg
      721 attcccccca gtaaatgaag accgtgcgcc ccggtaatca ggtaaaagaa aaagctgccg
      781 ggattggaag caaggtgcag tcctccggaa acgagttctc gccacgcgac aagctggccg
      841 gcaagaaaca caagccccaa tagtagagtg acgcgtatcc aacgagcgca tttctcgctc
      901 gcgtcgtcgc gcagcgagag ccgcgcaaat tccatcgtca gactgctgcc cagaaggatg
      961 actgtattga tgtacaggac atacggaagc ggaatcgaaa cccaatctgt cgaatgagcc
     1021 caacgccatt ccaaaacgag cgtcaacgtc acaaaaagag taacaatcca aataatcgcc
     1081 aagaatgtga tcaagcgata cgcgctgata ggtgttcccc ggggatcgag ttgtgcggtg
     1141 gcgtgaagct cataaacggg ctcgccggga ctgcctccgt ttcggtttcg attgttgccg
     1201 gatggtgaag accttcgtcc aggcgatgct cgctgagaac gtacgatggt agccatgtcg
     1261 attttctcta gtttcttcgg cagcctcttt tagtcatccc agtttcaaac cgggtggctc
     1321 cgctccgcgc gtcgaatcgg ataatatcac tgcgctggtc gagagagcag tccacagatt
     1381 atccgacgaa cgtgaggttt gcgcagaccg ccgctgattc ctcgccgagt tcgccagatc
     1441 accggcggct ttctttggaa tgccgctcga tcatgcgcgt ggtcagtaag acgagcccga
     1501 aaatctgagt ccactccgac acgatattgc cgacaacctg gccccatttc gcatccggat
     1561 tcattctggc gaacagcacg acccagccga ttcccgttat tagaagaaag atagtcagcg
     1621 aatgatcgcg aagccattcg agaactggag tgagcgtgtg tttcggcaga ggcttgcttt
     1681 cggcggatcc tttttcgtac aagtatttcg tcgcaatgac ggtaacaaca acgcccgacc
     1741 aatccgctat tgcgtttccg aagaacgacc cccaatgagt actgggattc gagaagacat
     1801 aaaagccaat ccacagaaaa aggatgccga tggaagcgat actcagcgag tgttcgtgaa
     1861 agaaacttcg cttgcggtgg tgttgctgcg ccactgacgc cttaggcgtt tgagccgatt
     1921 cagtcattca gttgctccat ttaacttctt gccggccggg ctccatgtct gtctgactat
     1981 taggatcgcg gcggctggcg atgcgaatgc agaaaactga aaggtaaatg aacgacacgt
     2041 gacaaccact gcgcacggtt cgggagtcaa ttctcgcgtt tgaacgggcg gaagtctagc
     2101 cgtcccctta acgcccgggg gcgcggagac aatgtctgcc acgcgcttgt tcgcgtggtg
     2161 gccagagtta aaaagttacg gagtgattgg gtgaagtggt tgggagcaca acggcgcggc
     2221 tcatcccgtg aaaccaaatt tcgagaaggg ttcgagatcc gttgccattt tgcttgaaga
     2281 caatcgcatc ataatcgtag ggagcagatc gttttaccca ctttccctga gccttggcaa
     2341 ccgtgtcccc ggcctttaga aagttgacgg taacctcggg gctgtaagtc tcccaattga
     2401 cgacgaactg ctcgatgggt gtttctgcca atccgtgagg cgccgtcgat gaaactaaag
     2461 tcaagtctcg ggactttcgt gggagcgaat gcgtcttaaa gccgtgggag cgtaaatttg
     2521 tacttgcgat ctgcatttgc tgagggtcgg gataaggtcc tgcgattacc tgatacgaac
     2581 ttgcccacat acggtgcttc tgaatcacga tggcgtgaat tccgatgcgc gccagatcgt
     2641 cgctcgcgct gcgagcttcg aattcgtcct tgaatctgcc gacatcgaaa tactttccga
     2701 aaggcacgcc cgagttaatc tccgcaacat cctccgcgtt ctctacggaa tggttcgtcg
     2761 gcttcactgc cataactgtg gttttgtcaa gggcgcgatc ggctgattcg ggtttcagag
     2821 tgctgcccgg ctcaggcggg acagggcccg gccgggccgc agcgaagtca atcaatggcg
     2881 atgccgcagg tgatggcgtg aacgaaattg ccggagcgat cggtttgggt gcgagcttgg
     2941 ccggtgcggg cctggccgga tgcgttgcgg gctggattag ggcctcctca aagtgcggag
     3001 ctgggaccgg agtggaatct gggactgaga tcgtagcgcg ccgctggatc gtcggtcggg
     3061 aagcctggga cgaagcagac gtgggcgcag g
//
LOCUS       DATNDT010000128         4484 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1471535, whole genome shotgun sequence.
ACCESSION   DATNDT010000128 DATNDT010000000
VERSION     DATNDT010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4484
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1471535"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..818
                     /locus_tag="VN788_04080"
     CDS             354..818
                     /locus_tag="VN788_04080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008097527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXU19744.1"
                     /translation="MTAISLKVNGHVHKVDVPPETPLLYVLSDDLQLRGPKFGCGLAQ
                     CGACTVIIQGKAVRSCVMPVSAVKNREVTTLEGIGTPERPHAIQQDFIDEGAAQCGFC
                     LSGVILSAKALLDDNPKASDEQIQEALSNVLCRCFAHTRMMRAIKRYQARRA"
     gene            815..3115
                     /locus_tag="VN788_04085"
     CDS             815..3115
                     /locus_tag="VN788_04085"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXU19745.1"
                     /translation="MKKELSRRDFLKASGILVVSFSASSVIEPLAAAQGPFDTHPSHV
                     NPDKLDSWLAVASDGTVTAYTGKCDLGQGMYTVQTQLVAEELCVSIEKVKLIQCDTSV
                     TPDQGTTSGSQSTPTNFNTRNLAQAAATAREALIGMAAQKLGVPGDQLTVADGAVSAA
                     GGRRVSYSDLVGGKHFDLAVSATAKRRSPSEWKVLGTSVPSLDRVALMTGTFEFVHNV
                     HVPGMVHGRVVRPPEIGATVEHVDEMSVQHIPGLIKVVVKNNFVGVVAEKQWQAAQAA
                     KDLKVTWKPGAGLPAQKELYDYIRKQASRDVMVVNSKDVDDKLKSAAHVLKATYSYPY
                     QAHGSMGSSCAVADVQADHATIWSPTQSVYPTQHGISMLTGLTAKNVRVIFVRGSGCY
                     GLNAADTVSYDAALLSQAVHKPVRVQLTRQDEFLSENYGSACVIEQQVGIDANGAIQA
                     WDCNAWSISLGGRPGYGKPGNVITGMLAGFDPEMVTPRAAAEPTGELRNGSNAVPSYV
                     AGCINGKCGGAGSIRSERVLMHSVPSPFFTGPLRSPLRLQNTFAHECFMDEVSTYAKA
                     DPVAFRLQHLSDARIIGVVKAAAQKANWVARPSPEHGNARGGKVTGRGIACVAYEGGN
                     GYAALIAEVEVDQASGRVQPKRFIAALDCGPISNPDGLRNQIEGGVLQGTSRALSEEI
                     TWDERKVTSIDWRSYHTLPLGIQAPIFESVLVDQPAAKATGAGETAITLAAAAIGNAI
                     FDATGARIRQVPFTAERVREALAARG"
     gene            3191..3481
                     /locus_tag="VN788_04090"
     CDS             3191..3481
                     /locus_tag="VN788_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19746.1"
                     /translation="MTKVRAALLALALCSALSLGACQNAKTQQENQRLKAQVADLQKQ
                     LGEMGNRVDEATTAKNELTKENAELKQENDRLKARHGKTKKAKSKRRRHRAA"
     gene            3546..>4484
                     /locus_tag="VN788_04095"
     CDS             3546..>4484
                     /locus_tag="VN788_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXU19747.1"
                     /translation="MTAEYSPLPRLFLDAVDRFANPRAQMYRPAGGWEAFSAGEMLRR
                     VAGLARELVALGIQSGDRVGIFAPNCPEWHVADFAIQGLGAVVVPVYFRESPERLTYI
                     LKDSGARIAFTAGEEQSHKIAECRPNLPALEHVIHVGSAMGSGTFDGVRGSAPSYSTL
                     RYQDLIAIVGGPEVTEYRRRAGEVTSDQLATIIYTSGTTGEPKGVMLTHANLSSNSRD
                     FTDDFELSPSDVALSLLPLAHVYERTIDYGNIFRGVSIAYVEQPETAAQALLEVRPTT
                     MAAVPRFYEKTYANIIEKGHRESGLKRKIFDDAMRVA"
BASE COUNT          978 a         1186 c         1445 g          875 t
ORIGIN      
        1 tgcgcatgct cccatttgac tccatgcgaa ctacagcggc atctttggcc acagccgggg
       61 gaccagcgga gagggcctga gtaaggtact cggagtccga cattcgacgc tgctgagcat
      121 cggtcgtggc tgcgacggca agcaccgaga tggcgaccgc gagaaatgtc tttaacattg
      181 tttcctccgt gaggtttcga ctggattccg cgtggctcgg aattgagcac attctcactt
      241 tcaaatgcgt tgttgtcaag cctctagggc ctcgatcaga aattcgaatt gcttcaagtc
      301 aagacgtgac ctcgcatgta aacttttgta agctgtgagc ttggagcgag acgatgactg
      361 ctatttcttt gaaggtgaac ggccacgttc acaaggtaga tgtacctccg gaaactccgc
      421 tactgtacgt gctcagcgat gacctgcaat tacgcggacc caagtttggc tgcgggctcg
      481 cgcagtgcgg cgcctgcacg gtgatcattc aagggaaggc ggtgcgttcg tgcgtgatgc
      541 cggtcagcgc ggtgaaaaac cgggaggtca ccacgctcga aggcatcgga actcctgaga
      601 ggcctcatgc gattcagcaa gattttatcg atgagggcgc ggcacaatgc gggttttgcc
      661 tgagcggcgt gattctttcg gcaaaggcgc tgctggatga caatccgaaa gcgtccgatg
      721 agcagattca ggaggcgcta tcgaacgtct tatgccggtg tttcgcgcat acgcggatga
      781 tgcgcgcgat caagcgttac caagcgaggc gcgcatgaaa aaagaacttt cccgccgcga
      841 ttttctcaag gcttccggca tactggtagt gagtttcagc gcgtcatcgg taatcgaacc
      901 attggccgcg gcgcaagggc ccttcgacac gcatccgtcc catgtgaatc cggacaagct
      961 ggactcatgg cttgcggtgg catccgacgg cacagtgacc gcgtacacgg gaaagtgcga
     1021 tctgggccaa ggtatgtaca cggtgcaaac gcagctggtc gcggaggaac tgtgcgtatc
     1081 gattgagaag gtgaagctga ttcagtgcga cacgtctgtg acgccggatc aagggacgac
     1141 ctcaggcagc cagtccacgc ccacaaattt caatacgcga aatttggcgc aggcggcggc
     1201 gaccgcacgc gaagctctaa tcggaatggc ggcgcagaag ctcggcgtgc ccggggatca
     1261 gcttacagtg gcggatggcg cggtgagcgc ggcgggcgga cgccgcgtga gctatagcga
     1321 cctggtcggc ggcaagcact tcgacctggc agtaagcgcg acggcgaaac gccgttcgcc
     1381 cagcgaatgg aaagtgctgg ggacatctgt gccatcgctg gatcgagtcg ccctgatgac
     1441 cggcactttc gaattcgtcc acaacgtgca tgtaccggga atggtccacg gccgggtggt
     1501 gcgtccgcca gagatcggag caacggttga gcacgtggac gaaatgtcgg tgcagcacat
     1561 tccggggctc attaaggtgg tggtaaaaaa taactttgta ggggtggtgg cggaaaagca
     1621 gtggcaggct gcgcaagcgg cgaaggatct gaaggtcact tggaagccgg gcgcgggact
     1681 gccggcacaa aaggaacttt acgattacat caggaagcag gcgtcacgcg atgtgatggt
     1741 cgtgaactcc aaagatgtgg acgacaaact gaaatcggcc gcgcacgttc tgaaagccac
     1801 gtattcgtat ccctatcagg cacacggttc gatgggttcg tcgtgcgcgg tggccgatgt
     1861 ccaggcggat catgcaacta tctggtcgcc aacacaatcg gtgtatccca cgcaacacgg
     1921 catctcgatg ctcacgggtt tgacggccaa gaacgtgcga gtgattttcg ttcgcggctc
     1981 gggctgttac gggctcaatg ccgcggatac ggtttcctac gatgcggcgc tgctatcgca
     2041 agctgtgcac aaacccgttc gtgtgcaatt aacccgacag gatgaattcc tttccgagaa
     2101 ctacggctcg gcatgcgtga tcgagcagca agttggcatc gatgcgaacg gcgcgatcca
     2161 ggcatgggac tgcaacgcgt ggtctatatc gttgggggga aggccgggct acggaaagcc
     2221 cggtaacgta atcacaggga tgctggccgg atttgatccg gagatggtaa ctccgcgggc
     2281 ggctgcggag cctaccggag aactgcgcaa cgggagcaat gcagtccctt cgtatgtggc
     2341 gggatgcatc aacggcaagt gcggcggagc aggttcgatc cggagtgagc gggtgctgat
     2401 gcactcggtt ccgtcgccgt tttttaccgg accgttgcgc tcaccattgc ggttgcaaaa
     2461 caccttcgcg catgaatgtt tcatggacga ggtgagcacc tacgcgaagg ccgatccggt
     2521 ggcgttccgg ctgcaacact tgagcgacgc gcgaatcatt ggcgttgtaa aagccgcagc
     2581 gcagaaggcg aactgggttg cgcgtccatc gcccgagcac ggtaatgcgc gcggcggtaa
     2641 ggtaactgga cgcggtattg cttgtgtcgc gtacgagggc ggcaacggat acgcggccct
     2701 gatagctgaa gtggaagtgg atcaggcgag cgggcgagtg cagcctaaaa gattcatcgc
     2761 ggcgctggat tgcgggccga tttccaatcc cgacggcttg cgtaatcaga tcgagggtgg
     2821 cgtgctgcaa ggaacgagcc gcgcgctgag cgaagagatc acctgggacg aacgcaaagt
     2881 gacgtcgatc gactggcgga gctatcacac tttgccgctt ggaatccaag cgcctatctt
     2941 cgagagcgtt cttgtcgacc agcccgcggc gaaagcaacg ggcgcaggag agacagcgat
     3001 tacactggct gccgcggcga ttggaaacgc gatttttgac gccaccggtg cacgaatccg
     3061 gcaagtgcca ttcacggccg aacgtgtgag agaagcgctg gccgcgcgcg gatgagagac
     3121 acccgctaac agagctataa cgatccaagc attggatcga attcgttggg atttttcgag
     3181 ggaggcgtac atgacgaaag tccgcgcggc tttgttggca ttggctcttt gctccgctct
     3241 ttcgctcggt gcctgccaga atgcgaagac gcaacaagaa aaccagcggc tcaaggccca
     3301 agttgccgat ctccaaaaac aacttggtga gatgggcaac cgcgtggacg aagctacgac
     3361 cgccaagaat gaattgacga aagaaaacgc cgagctaaag caggagaacg atcgcctgaa
     3421 ggcgagacac ggcaaaacga aaaaggcgaa atcgaaaagg cgccgtcacc gggcagcgtg
     3481 agggcttgtt agccgtggtg cgagccgcat tgcgcaacag ttccggctag gctagagtgg
     3541 cgcgcatgac cgccgagtac tctccgcttc ctcgattatt tctggatgct gtggaccgtt
     3601 tcgcgaatcc gcgggcgcag atgtatcgcc ccgccggggg ctgggaagca ttttcggcgg
     3661 gtgaaatgtt gcggcgcgtg gcgggactgg ctagggaact ggtcgcgctt ggcatccaaa
     3721 gcggcgaccg ggtgggaatt ttcgcgccga actgtcccga gtggcatgtg gccgattttg
     3781 caattcaagg ccttggcgct gtggttgtgc ccgtctattt ccgcgagtcc cccgagcggc
     3841 ttacctacat tttgaaggat tcgggagcgc gcatcgcttt cacggcagga gaagaacagt
     3901 cgcacaagat cgccgaatgc cgcccgaatt tgcctgcgtt agagcacgtc attcacgtgg
     3961 gttccgcgat gggctccggt acgttcgatg gggtgcgggg aagcgcgccg agctacagca
     4021 ctttgcgcta ccaagacttg attgcaatcg tcggcggccc ggaagtcacg gaatatcgcc
     4081 gtcgcgcggg ggaagtaacc tcggatcagc ttgcaacgat catttacacc tcgggcacca
     4141 cgggcgagcc caaaggtgtg atgctgactc atgcgaatct cagctcaaac tcacgcgact
     4201 ttactgacga tttcgagttg tcgccgtcgg atgtggcgct gtcgctgttg ccgctggcgc
     4261 acgtgtacga gcgcaccatc gattacggga atatttttcg aggggtctca atcgcgtatg
     4321 tggaacagcc ggaaaccgca gcccaggcgt tgctggaggt ccggccgacg accatggcgg
     4381 ccgtgccgcg cttttacgaa aagacctacg cgaatatcat cgagaaaggg catcgagaat
     4441 cggggttgaa acgcaagatc ttcgacgacg cgatgcgcgt ggcc
//
LOCUS       DATNDT010000129         3892 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4063568, whole genome shotgun sequence.
ACCESSION   DATNDT010000129 DATNDT010000000
VERSION     DATNDT010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3892)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3892)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3892
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4063568"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            207..1577
                     /locus_tag="VN788_04100"
     CDS             207..1577
                     /locus_tag="VN788_04100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXU19748.1"
                     /translation="MKRLYQIMLIALGLVTLLFIVARNGRAIAAQFTRPPAHAVDPNA
                     KPATPGVFTDFKGEEPGKIHHITAADIPGPYATKSAAIFSRPVPKPADAWPQAPAGFK
                     VTLYAEKLADPPRTIVTAPNGDFFVVSTNKGKVLVFHGMTADGKPEKTEEFASGLKLP
                     FGIAFYPPGPNPKYVYIGDTDAAVRYPYQNGDMKARGPQEVVVSNLPAGPNHFARGVA
                     FSLDGKQMFISVGSHSNVPPESDPDPDPLEFHRADILIADPTAVNQDGKDLQVYASGI
                     RNGSGIAVDPKTGELWTSVNERDEIGDNVPPDYITHVQKGGFYGWPWYYTGGHPDPRF
                     PGKHPELKDKVIVPDVLLEPHNASLNITFYEAKQFPKQYQGQLFAAEHGSWNRSVRTG
                     YEVIFVPMKNGKATGAYEDFLTGFLTPSGDCWGRPVGVAVAADGSLMVTDDGSNSIWR
                     VTYGKK"
     gene            1630..3036
                     /locus_tag="VN788_04105"
     CDS             1630..3036
                     /locus_tag="VN788_04105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HXU19749.1"
                     /translation="MAAAVSLAALLLGGCKVGPRYVRPTADVPPQYKEAGNWMPAQPS
                     DAISKGQWWRIYGDPQLNTLEDQVTVSNETLKAAQAQFLEARAAVRVARSPFFPVVSG
                     GASASRIRTSQNRPSVTSAKTLNDFQLPLDVSYEPDVWGRVRRSVEAARSEAQATAAD
                     LASTDLSLHAELALDYFQLRGLDSEQQLLNSTVTSYERALQLTRSRYQGGIASAVDVA
                     QAQTQLETTRAEAEDVAVQRAAFEHAIAALIGKTSYEFTQQPLPLAYAPPPIPVGLPS
                     ELLERRPDVAAAERRVQEANAEIGVARAAYFPSISITGSGGFESAKIGNLLEGPSGFW
                     SLAGSAAELIFDGGQRRGINEQARASYDKFVDNYRQTTLTAFQEVEDNLAALRILDTE
                     TSTQDAAVAAAQHSLDLSNTRYRGGVANYLEVTTAQSAALGDERAAVDVRTRRLVASV
                     LLIKALGGGWNASQIPQI"
     gene            3337..>3892
                     /locus_tag="VN788_04110"
     CDS             3337..>3892
                     /locus_tag="VN788_04110"
                     /inference="COORDINATES: protein motif:HMM:NF025878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19750.1"
                     /translation="MRCISTDGIKESDRCEFWRANTVPVFGPLDIELLENQVFDGNFQ
                     FTTVSDLIFSRLASRTPHRVVLSGLSALHSQGFVKALFLTDGRSILQQNGRTAILRPG
                     EWSVYDTAAPCSLTVPDRSEMLVVLIPRERLLARGLDLHDLVARRFSGRHGLGKLIWS
                     LISNTFNEIPQIGRRSNRDVANIVV"
BASE COUNT          793 a         1250 c         1018 g          831 t
ORIGIN      
        1 gaggtcgaag tcgtatccgg catctcagca gccgattcca ttatcgaaac tccgccggac
       61 tccctggtta cgggtgaaac cgtgcgcgta gtcgccgccg cacgtattcc gcaacgcgag
      121 ccggccggaa attccgaata ataaatgggc cggcttcacg taaaattaaa actcgggaag
      181 catcgaagtt taagggggga agaaccatga agcgtcttta tcaaatcatg ctgatcgctt
      241 tgggcctcgt aacgctgttg ttcattgtcg cgcgaaatgg acgcgcgatc gccgcgcagt
      301 tcacccggcc gccggcacac gcagtagatc cgaacgccaa gcccgccact ccgggcgtgt
      361 ttactgattt caagggcgaa gagccgggca aaatccatca cattacggct gcggatatac
      421 cgggacccta cgccacgaaa tccgcggcca ttttctcgcg ccccgttccc aaacccgcgg
      481 acgcgtggcc gcaagcgccg gccggcttca aggtaacgct ctacgccgaa aaattagccg
      541 atccgccgcg aaccatcgtc actgctccca atggcgattt cttcgttgtc tccacaaaca
      601 agggcaaagt cctcgtcttc cacgggatga ccgccgatgg caagcccgag aaaactgaag
      661 agttcgcctc aggcctgaag cttcctttcg gtatcgcgtt ctatcccccg gggccaaatc
      721 ccaagtacgt ctatatcggc gacacggacg ccgctgtccg ctacccctat caaaatggcg
      781 atatgaaggc ccgcggtcct caagaagtcg tggtttccaa tcttcctgcc ggcccgaatc
      841 acttcgcccg cggcgtggcc ttctccctcg acggaaagca aatgttcatt tccgtcggct
      901 cgcacagcaa tgtgccgccg gaatcggatc cggatcccga tccgctggaa ttccaccgag
      961 ctgacattct catcgccgat cccacggctg taaatcagga tggaaaggat ttgcaagttt
     1021 acgcttctgg catccgcaac ggctccggga tcgctgtcga tccaaaaacc ggcgagcttt
     1081 ggacgtccgt aaacgagcgc gacgaaatcg gtgacaatgt ccctcccgat tacattactc
     1141 acgtccagaa gggcggcttc tatggttggc cttggtatta cactggcggc catcccgatc
     1201 ctcgctttcc cggtaagcat ccagaactca aagacaaagt cattgttccc gatgtgcttc
     1261 tcgagcccca taatgcttcg ctgaacatta ccttctacga agcaaagcag ttccccaagc
     1321 aatatcaggg gcagctattt gccgccgaac acggctcgtg gaatcgctcc gtccgtaccg
     1381 gctacgaagt cattttcgtg cccatgaaga atggcaaggc cacgggcgcg tacgaggatt
     1441 ttctcaccgg ctttctcacc ccgtcgggag attgctgggg ccggcccgtt ggagtcgcgg
     1501 ttgccgcgga tggctccctt atggtgacgg atgacggctc gaattcgatc tggcgcgtca
     1561 cttacgggaa aaagtaggat cgtggttttc ggtcacgggt tggaaaaaca tacctcgcgg
     1621 tgcatccgct tggcggcagc cgtaagcctc gcggcgcttc tgctcggcgg atgcaaggtc
     1681 ggacccaggt acgtccggcc cacagcggat gttcccccgc agtacaagga agcgggcaat
     1741 tggatgcccg cacagccgag tgacgctatc tccaagggcc aatggtggcg gatttacggc
     1801 gatccgcagc tcaacactct cgaagatcaa gtcaccgtct ccaatgaaac gttgaaagcc
     1861 gctcaggcgc aatttctcga ggcccgggca gcggttcgcg tggcgcgctc tccatttttc
     1921 cccgttgtca gtggcggagc ctctgcttcc cgaattcgca cctcacaaaa tagaccctcg
     1981 gtcacttctg ccaaaactct taacgacttt caattgccgc tcgatgtttc ctatgagccg
     2041 gacgtctggg gccgcgtccg ccgcagcgtg gaagcagctc gttccgaggc ccaggcgact
     2101 gccgccgatc tggccagcac ggacctcagc ctccatgccg aactcgctct cgattatttc
     2161 caactccgag gcctcgattc ggagcagcag ctcctgaact ccactgtcac gtcgtacgaa
     2221 cgcgccctcc agttgactcg cagccgttat cagggcggca ttgcctccgc ggtggacgtc
     2281 gcccaggccc aaacccagct tgaaaccacc cgcgccgagg ccgaggatgt cgccgttcag
     2341 cgcgcagctt tcgagcacgc cattgccgcg ctgattggca agacaagtta cgaattcacc
     2401 cagcagcctt tgcctctcgc ctatgcccct ccgcctatcc ctgtcggtct gccctccgaa
     2461 ttgctcgagc gccgtccaga tgtagccgcc gccgaacgcc gcgtccagga agccaatgcc
     2521 gagatcggag tcgctcgcgc cgcctatttt ccgtccatct ccatcacggg atcgggcggt
     2581 tttgagagcg ccaaaatagg taatcttctc gaagggccta gcggattttg gtcccttgcg
     2641 ggatcggccg ccgaattgat tttcgatgga ggccagcggc gcggcatcaa cgagcaagcg
     2701 cgcgcctcct acgacaaatt cgtggacaac tatcggcaga caaccttaac cgcctttcaa
     2761 gaggtcgagg acaatctggc tgccttgcgt attctcgata ccgaaaccag cacccaggac
     2821 gccgcggtcg ccgccgcaca gcattctctc gatctctcca acacccgata caggggcggc
     2881 gtcgccaact atctcgaagt aactaccgct cagagcgccg ctctcggcga cgagcgtgcc
     2941 gccgttgacg ttcgcactcg ccgcctggtc gccagcgttc tccttatcaa agccctcggc
     3001 ggcggctgga acgcctctca gattcctcaa atctagtttc ccatcgccga gagcaaaagg
     3061 accggtcttt tgattcgtgg gggcacgggc atgccgtgcc cggcaacaat acttggagct
     3121 atgtccacaa ttttgctctt cacaaaagac gggttccatg tctgcctcca ccgaacgggc
     3181 gacaatggtt tccttttgat cctaagattc ccctcaggcc ctagctccgt ccgaatgcca
     3241 aaaaactaga cagtatcggc gcattttcga taatatgcag ccgtgatccc cgcccccgct
     3301 gcttccgcgg ttgcattggc agaccatgcg ccggccttgc gatgcatttc caccgacggc
     3361 atcaaggaat ccgaccgctg cgaattttgg cgcgccaaca cggtacccgt tttcggtccg
     3421 ctcgatatcg agctgttgga aaatcaggtc tttgacggca actttcaatt cactacagtg
     3481 tcggatctga tcttttcgcg tctcgcatca cgaacgcccc atcgcgtcgt cctctctggt
     3541 ttatccgccc tgcatagcca ggggttcgta aaggccctgt tccttactga cggacgcagt
     3601 atcctccagc agaatggacg caccgctatc ttgcgacctg gcgaatggag cgtttatgac
     3661 accgctgcgc cttgttccct cacagttccg gatcgctcgg agatgcttgt tgttctgatt
     3721 ccccgcgaaa ggctcttagc tcgtggtctc gatctccacg atcttgttgc caggcgcttt
     3781 tccggtcgcc atggactcgg caaactgatt tggagcctga tctccaacac ctttaacgaa
     3841 atcccacaaa taggtcgccg ctccaatcgc gacgtcgcca atatcgtcgt tc
//
LOCUS       DATNDT010000130         3465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1775643, whole genome shotgun sequence.
ACCESSION   DATNDT010000130 DATNDT010000000
VERSION     DATNDT010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1775643"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1242)
                     /locus_tag="VN788_04115"
     CDS             complement(139..1242)
                     /locus_tag="VN788_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HXU19751.1"
                     /translation="MSAYRADQVGSFLRPPQLLEARRADPPNPARLHEIEDAAIERVL
                     AKQKELGFDVFTDGEFRRSNFMGDFTDAVKGFDFSDGVARSWDTAQAGGSLLGRGRVA
                     GVVVEKLQVVRPLTGHELPFLKQHSPGAIKMTLPSATQFPAISYKRGRSDKAYPSYSA
                     MLWDIVEIMKKEMARLASEGVKYIQIDAPRYSYFMDPKWREWIRTEMQVEPDAWLDES
                     IKADNACFEAARKPGVTLAIHLCRGNSRSHWYAEGGYDAIAEKLFGTLAVDRFLLEYD
                     DARSGTFEPLRFVPHTKTAVLGLISSKRPELESGDQIIARIKEASRHLPLENLALSPQ
                     CGFASTMEGNLVSEDDQWAKLRLVVETAHKVWS"
     gene            complement(1297..2322)
                     /locus_tag="VN788_04120"
     CDS             complement(1297..2322)
                     /locus_tag="VN788_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013092460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU19752.1"
                     /translation="MIIDVHGHITAPDALYVYKANLVSHRGSHGRGKVEISDDEMIAF
                     LNKPTFGTGKSHLDLLKEVGTDMQLISPRPFQMMQAEKPPRVSQWFIEECNNLVAQQC
                     RLFPKVFRGICGLPETPGVSPKNCIPELERCVKEFGFVGCLISPDPSEATDYETPGLG
                     DEYWYPLYEKLVELDVAGYIHGAGCRSIRHSYSVHFINEETISVVSLVNSRAFQDFPK
                     LKLVCSHGGGAVPYHAGRFLAPSLRRGQSTFMENLRKLYYDTVLYTPLACELLIKTVG
                     ADRCVFGTERPGVGTAKNPQSGKWMDDVRVYIDGFDWLSEADKKTIFEDNAKKIFRLG
                     AEAKTAA"
     gene            complement(2532..3161)
                     /locus_tag="VN788_04125"
     CDS             complement(2532..3161)
                     /locus_tag="VN788_04125"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HXU19753.1"
                     /translation="MRFKMIAIFIFVISLSIFAGATAAQAPTSDPGAQASTGQSGAQG
                     QQESGDRARPEQRRMRREMRDGGMMMGRQRGGRRGEGLGGPLQRVSKLLAALDDPRVR
                     TVLGLTDQQADSLRKIIVGTETFTIKTGADIAVDSIDLRELLRADKPDRAAVMSKGDE
                     ISKYTSQLINHYLDAMLAAKAVLTPAQQKMIRAYIERGAPALSAPAARP"
BASE COUNT          721 a         1115 c          839 g          790 t
ORIGIN      
        1 tttggattct tccggtccgc ctcgccgcgc aaatgccaca tgtcgggagg cgttgaaagc
       61 atcggcccgt gcgatgaccc catgccgagc accacttttg ccatgtcctc ctccgaattt
      121 cctcggcgcc gaaaaaccct agctccagac cttgtgcgcc gtctccacaa ccagccgcag
      181 tttagcccac tggtcgtctt cgctgactaa atttccctcc atcgtcgagg cgaagccgca
      241 ctgcggactc aatgccaaat tttcaagcgg caggtgcctc gaagcttctt tgatccgcgc
      301 gatgatttga tcgccgctct ccagttcagg ccgcttagag ctgattagtc caagcacagc
      361 cgtcttagtg tgcggcacga atcgcagcgg ctcgaaagtg cctgagcgcg cgtcgtcgta
      421 ttccagcagg aaccggtcca cggccagtgt tccaaaaagt ttttccgcga ttgcgtcgta
      481 gccgccttcg gcataccaat ggctgcggct gtttccccgg cacaagtgaa tcgccagcgt
      541 cactcccggc ttccgcgccg cctcgaagca agcattgtcc gccttaatcg actcatcgag
      601 ccacgcatcc ggctcaacct gcatctccgt gcgaatccac tcgcgccatt tcgggtccat
      661 gaaataactg tagcgcggcg catcgatctg aatgtatttc acgccctctg atgccagtcg
      721 cgccatctcc ttcttcatga tttcgacaat atcccacagc atcgccgaat agcttggata
      781 agctttgtcg gacctgccgc gcttgtatga aatcgcgggg aactgcgtag cgctcggcag
      841 tgtcatcttg attgcgccgg ggctgtgttg tttcagaaat ggcagctcat ggcctgtcag
      901 aggccgcacc acctgaagtt tttcgaccac cactcccgcc actcttcccc gcccaagcaa
      961 cgacccgccc gcctgcgcgg tatcccagct gcgtgccacg ccatcgctga aatcaaatcc
     1021 ttttaccgca tcggtaaaat cgcccatgaa attgctgcgg cgaaattcac catcggtaaa
     1081 aacatcgaac cccagctctt tctgcttggc aagcacacgc tcgatcgcgg catcttcgat
     1141 ttcatgcaat cgcgctggat tcggcggatc ggcccgcctc gcctccaata gctgcggcgg
     1201 ccgcaaaaaa ctacccactt gatcggccct gtacgctgac attcttaaat ttctcttttc
     1261 tatttgtatt ccagcgtctt cactcgcagc cgaatcctac gcggccgttt tcgcttcggc
     1321 acccaggcga aagattttct tggcattgtc ctcgaagatc gtcttcttat cggcctcgct
     1381 gagccaatcg aatccatcaa tataaacgcg cacgtcgtcc atccactttc cgctctgcgg
     1441 atttttcgcg gtgcccaccc ccggacgctc ggttccgaaa acgcagcgat cggcgccaac
     1501 tgtcttaatc agcaactcgc aagccagcgg agtgtagagc accgtgtcgt aatacaattt
     1561 ccgcaaattt tccatgaacg tgctctgccc gcgcctcaag cttggcgcca gaaaacgccc
     1621 cgcgtgatac ggaaccgctc cgccgccatg cgagcacacc agtttaagtt tcggaaaatc
     1681 ctgaaatgcg cgcgaattga ccagcgaaac caccgaaatc gtttcctcgt tgatgaaatg
     1741 cacggaatag ctgtgacgga tcgagcggca cccggcgcca tgaatgtatc ccgccacatc
     1801 cagttccacc agtttctcgt agagcggata ccaatattcg tcgcccagtc caggcgtttc
     1861 gtagtcggtt gcctcgcttg gatccggact gattaagcat ccgacaaatc cgaattcctt
     1921 cacgcacctc tccagctccg gaatgcaatt tttcggactg acgcctggcg tttccggcag
     1981 cccgcaaatc cctctgaaca ccttcggaaa aaggcggcat tgctgcgcga ccagattgtt
     2041 gcattcctcg atgaaccact gcgaaactct cggcggcttt tcggcctgca tcatctgaaa
     2101 tggacgggga gaaatcagtt gcatgtccgt gccaacttcc ttcagcagat ccaaatgcga
     2161 tttacccgtg ccaaaagttg gcttgttgag gaaagcgatc atctcgtcgt cgctgatttc
     2221 cactttgccg cgcccatgcg atccgcggtg cgaaaccaga ttcgccttgt acacatacag
     2281 cgcatcaggc gccgttatat gtccgtgaac gtcaatgatc atgccaccct ccccgcctca
     2341 aggtgtttca ctgcgaacgg tcttaatatc gccgaatgca cgggatgtca aattgattcc
     2401 cgtcgtcggc gaaagcgttc atccccgctc aaaggcccgt ccaaaataga cagccatgcc
     2461 tccgtcagca tgacgacgcc ggtaagtcga acttgtatcg aaagcaggcg gtacctctta
     2521 ccgcgcccgc tttacgggcg agcggcaggc gccgaaagag caggagcacc acgctctatg
     2581 taggcccgaa tcatcttttg ttgcgccggc gtcagcacgg ccttcgcggc cagcatcgcg
     2641 tccaggtaat gatttataag ttgcgacgtg tatttggaaa tctcatcgcc tttgctcatc
     2701 acggcagccc tgtctggctt gtcggctcga agaagttccc gcagatcgat cgaatcgacg
     2761 gcgatgtcgg ctcccgtttt gatcgtgaat gtctccgtac cgacgatgat ctttcgaagg
     2821 ctgtcggctt gttgatcggt gagaccgagt acagtacgaa cacgcggatc gtccagcgcc
     2881 gccagcagct tcgaaactct ctgaagaggt ccgcccaatc cttcaccgcg ccttccgccg
     2941 cgctggcgcc ccatcatcat gcccccatcc ctcatctctc tacgcatgcg tcgctgctcc
     3001 ggacgcgctc gatcacccga ttcttgctgg ccctgtgccc ccgattgacc ggttgaagcc
     3061 tgcgccccag gatcggacgt cggagcttgg gcagcagtgg cgcccgcaaa tatcgaaagg
     3121 cttattacaa aaataaaaat ggcaatcatc ttgaatcgca tcaatgtccc tcccatcggc
     3181 tcacgccctc gcaatccgaa cttccgcttt gcccggcgcg cccgatgcca ataatcgtaa
     3241 gtatacccct tggatctcgc agaaacgttt cccactaaca gtggacggcc gtgcccgccc
     3301 tagccggatt ccccgctttt ggggccccat acagcctttt ccaaaatgtc acatcttaat
     3361 aacggacaat acttgcgatc tccctgcgag atcgcgggcc tatgttaaac tcggccctag
     3421 tggattgcgg cggcgcgccg agcgatcaaa aatggctgaa tttgt
//
LOCUS       DATNDT010000131         4896 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1759906, whole genome shotgun sequence.
ACCESSION   DATNDT010000131 DATNDT010000000
VERSION     DATNDT010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4896
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1759906"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VN788_04130"
     CDS             <1..607
                     /locus_tag="VN788_04130"
                     /inference="COORDINATES: protein motif:HMM:NF018454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion IcmF C-terminal
                     domain-containing protein"
                     /protein_id="HXU19754.1"
                     /translation="ASQELALGDFVGFFQPGSGTFSKFLDQNKAVYELQNGQYVQKSG
                     NPQPEVTKLVNRVADIQRALFPAGATAPQFKFSVKAHPQPEISSETLTIEGQPLKVAG
                     NHEGEKGFVWSGAGGEASLSINGTSFGEFQGPWATFRLFDYYNWTAGPAGYHLSWPVR
                     GFGGQQAKINGKPLVAEFDLDSGNIPLFQRNYLAALKCPAH"
     gene            complement(609..2222)
                     /locus_tag="VN788_04135"
     CDS             complement(609..2222)
                     /locus_tag="VN788_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HXU19755.1"
                     /translation="MKRRDFLKSAAVANALPFLPKGAAAQSGTPRQVVFILGESVRWD
                     MLNCYRQTGLKTPHLDRLAAAGMRFDRAYNCQPVCAPARSAIWTGLFPHTNGVWGNSM
                     PLGDTVHSIGQRLHDRFIHTAFIGKWHLDGVDYFGTGRPPDGWDPEYWYDMRDYLTEL
                     SPADRVRSRQPKTGSDPTWTADMCFAHRVTNRALQFLVDNKEKEFMLCVCYDEPHGPS
                     LCPKQYSDAYENFVFPSSANVKDPLTNKPAEQVIWAGPRINEQPPPIRAKQFFGSHTF
                     VDAEIGRLLDQIEQSAPNALIIFTSDHGVFLQSHRLLDKGPAMYEEITRIPFIVRWPG
                     HTPAGSVNKNLVSHVDLAATLMDFWGFDVPATLEGVSVLNGFRDTQAPTRDAVFLEWG
                     RYEIDHDGFGAFQPIRCICDERYKLSIHLLTTDALFDLQSDPDEMNNLIDSPAHAEIR
                     NHLHDKLLDWMNTSRDPFRGYYWGRRSWRPDFPETWGNAGFTRQRESDGYLPRELDYD
                     TGLTMTEATRPKKDGATNSYPPAREPNAQ"
     gene            complement(2320..2395)
                     /locus_tag="VN788_04140"
     tRNA            complement(2320..2395)
                     /locus_tag="VN788_04140"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2360..2362),aa:Val,seq:tac)
     gene            2533..2655
                     /locus_tag="VN788_04145"
     CDS             2533..2655
                     /locus_tag="VN788_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19756.1"
                     /translation="MKKILNEDLPSISVALQALVGNIIMVGAAALLLYFATGKW"
     gene            complement(2704..3216)
                     /locus_tag="VN788_04150"
     CDS             complement(2704..3216)
                     /locus_tag="VN788_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU19757.1"
                     /translation="MTAQQQPEPWLRGTLTDVAAVPRAVLHALELAREDIFRWCSGLT
                     DDELNARPAGIASVAFHIRHIVRSADCLLTYAEGVQLTPAQLDAMKSELDPGATNATV
                     FSEFNEGIERASARIRAIGAGHLEESRKVGRKELPTSVGGLLVHVADHTQRHVGQAIT
                     TAKIVIAARA"
     gene            4098..>4896
                     /locus_tag="VN788_04155"
     CDS             4098..>4896
                     /locus_tag="VN788_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19758.1"
                     /translation="MASQSRRISIAIHPEQALGETPADFLGLGYEISSVARPGLLSAR
                     NTAYVQFVRNLGRRGVIRIGGNTSDFSSYAKDGPAVCATKGTVVNDAVIRDLGEFLDA
                     TGWQLIWGLNLGSRDLANAIEQATSVIAGTKDKLLAFEIGNEPDLFAGGVAHRPKGYS
                     YADYLKEYREYKTALRAKFPKSPFAGPDVASHTDWLTQFAADEGSDLKLLTHHHYAEG
                     PPTSPASTIERLLTPSEGLTRMLNQCEAASRSAGVPYRICETNSCFGG"
BASE COUNT          978 a         1273 c         1515 g         1130 t
ORIGIN      
        1 ggcatcgcag gaactcgccc ttggcgattt tgtcgggttc ttccagccgg gcagcggcac
       61 attttccaaa tttctcgatc agaacaaggc cgtgtacgaa ttgcagaacg ggcaatatgt
      121 gcagaagagc ggcaatccgc agcccgaggt gactaagctg gtgaatcgcg tggcggacat
      181 ccagcgcgcg ctctttccgg ccggcgcaac ggcgccgcaa tttaaattca gcgtgaaggc
      241 gcatccgcag ccggaaatca gtagcgagac tttgacgatc gagggccagc ctctcaaggt
      301 cgccggcaac catgagggcg aaaaggggtt tgtttggtca ggagcgggcg gggaagcgag
      361 cctcagcatc aatgggacga gttttggcga gttccagggc ccgtgggcaa cgttccggct
      421 cttcgattat tacaattgga cggcaggacc cgctggctat catctctcct ggcccgtgcg
      481 cggattcggc gggcagcagg caaaaattaa cggcaagccg ctagttgctg aattcgattt
      541 ggattccggc aacattccgc tgtttcagcg gaactatctt gccgcgctga agtgcccggc
      601 gcactgatct actgggcgtt aggttctctt gcaggcggat agctgttcgt cgcgccatct
      661 ttcttcggac gcgttgcctc ggtcatcgtc agccccgtgt cgtagtcaag ctcgcgcgga
      721 agatagccgt cgctttcgcg ctgtctagtg aatccggcgt tgccccacgt ttccggaaaa
      781 tccggccgcc acgaacgacg tccccaataa tagccgcgga aaggatcgcg cgacgtattc
      841 atccaatcga gcagcttatc gtggagatgg ttacgaattt cggcgtgcgc gggcgaatcg
      901 atcaggttgt tcatctcatc gggatcggat tgcaaatcga aaagcgcgtc ggtggtaagg
      961 agatggatcg atagcttata acgttcgtcg cagatgcaac gtatcggctg gaatgcgccg
     1021 aagccatcgt gatcgatttc gtagcgcccc cactccagga agacggcatc gcgagttggc
     1081 gcttgcgtat ctctgaagcc gtttagcacg ctcacgccct cgagtgtcgc cggcacgtcg
     1141 aatccccaaa aatccattag ggttgccgcg agatcgacgt gggaaacgag atttttgtta
     1201 acggaacccg ccggtgtgtg tccgggccac cggacgataa acggaatgcg cgtgatttcc
     1261 tcatacatgg ccggtccctt gtccagcagc cgatgggact ggaggaacac gccgtgatcg
     1321 gacgtgaaaa tgatcagcgc attgggcgcg ctttgctcga tttggtccag cagccggccg
     1381 atttccgcat ctacaaacgt atgcgaaccg aaaaactgtt tcgccctgat cggaggaggc
     1441 tgttcattaa tgcgcggccc ggcccaaatg acctgctcgg ccggcttgtt cgtgagcggg
     1501 tcttttacat tggcgctcga agggaacacg aaattttcat aagcgtctga gtattgtttc
     1561 gggcacaggc tcggtccatg cggctcgtca tagcagacac agagcatgaa ttccttctct
     1621 ttattatcaa cgagaaactg gagcgcacgg ttcgttacgc gatgggcgaa gcacatatcc
     1681 gccgtccacg tgggatcgga gccggttttg ggctggcggg agcgaacgcg atcagccggc
     1741 gaaagctcag tgaggtagtc gcgcatgtcg taccaatatt cggggtccca tccgtcggga
     1801 ggacggcccg tgccgaaata atccacgccg tcgagatgcc atttgccgat gaacgcggtg
     1861 tgaataaaac ggtcatggag gcgctggcct atcgaatgga ctgtatcgcc gagaggcatg
     1921 ctgttgcccc agacgccgtt tgtgtgaggg aataaaccgg tccagattgc cgagcgcgcc
     1981 ggtgcacaga ccggctggca gttgtaggcg cgatcaaatc gcattccggc tgccgcgagc
     2041 cggtcgaggt ggggcgtctt cagtccggtc tgtcgataac agttgagcat gtcccagcgg
     2101 acagactcgc cgaggatgaa tacaacttgt cgaggagtgc cggactgtgc cgctgcgcct
     2161 tttggcaaaa atggcagggc gttcgctact gctgcggact tcagaaaatc tcttcgcttc
     2221 atggagtgcc cccaaagccg gaattaccct agttggggag caaatatatt gtgcttcgag
     2281 cgattgtcaa ctcgaatcga ggatcgctgg ctgcggcgat ggtgggcggt tagtgatttg
     2341 aacactagac cttccgggtg taaaccggat gctctaaccg ctgagctaac cgcccgcgta
     2401 gaataagtta gcatagattg tgcgcgattt tggtgactcg gcatcttggg tcttgatgca
     2461 agcgcgcgcg ctctataaaa tgtgggttca tcgtaagcat aagttgggta gtgatctggg
     2521 tcaggggaga caatgaaaaa gattctgaac gaagatctgc cgtcgatttc cgtcgcgctc
     2581 caggctttgg tcggcaacat cattatggtt ggcgcggcgg cgcttcttct ttacttcgca
     2641 acgggcaaat ggtaattgcg cggatcggct gctgcgcgtt gtaaacgacg gatttcgttc
     2701 ggtttatgcg cgtgcggcga tcacgatttt tgcggtggta attgcctggc ctacgtggcg
     2761 ctgcgtgtga tcggcgacgt gaaccagcaa cccgccgacc gaagttggca attccttcct
     2821 tccgactttt cgagattcct ccagatggcc agcaccgatg gcgcggattc tcgcgcttgc
     2881 gcgctcgatt ccttcgttaa attcagagaa tacggtggcg ttcgttgcgc ccggatcgag
     2941 ttccgatttc atggcatcta gctgtgccgg cgtgagttgc acgccttcgg cgtaggtgag
     3001 caggcagtcc gcgctgcgta cgatgtggcg aatgtgaaat gcgaccgacg cgattcctgc
     3061 cggccgcgcg ttcaattcat cgtccgtgag gccgctgcac cagcgaaaga tgtcttcgcg
     3121 ggcgagttcc agcgcgtgaa gcacggcgcg cggcacggca gcaacgtcag tgagagtgcc
     3181 gcgaagccac ggttcgggtt gttgttgggc tgtcattttg ccattttgcg ccgccttggc
     3241 gcgccatatc acgctttatt taggttaaat ggccaaaatt gcgacgtttt ggcacttgtg
     3301 acaccgaaac attgcagctt cgatactaca agtctccaag gagagcgatt gttgcgtgcg
     3361 ccatgcgggt ttctggatca cccgatcggc tgttttcttt ggatttcgtc tagaaaattc
     3421 cacctttttt ctcgattaga atcgcggttt ttattaaggc gcaatgactt agattgattt
     3481 ctaatcgagg cttctcgatt aggtttttct tcgcctcctt ccggcacgcc tcctctccat
     3541 tcaaaaaccg agcaaagtta gcacgctcac agagcgattt ctattgtgga aaatacggac
     3601 atagtactta ggtgggtgcg cggtgttgtt gccgggtcac cgttggtctt tcatcggcat
     3661 atagaggttt gtgccgcgcc tacggcgctc tgttgtggtt ggcgcgccca cccagcgctt
     3721 acgcggctgg gctaagttgt gccgcgctta cagcgctggt ctgtgtgccg aagcggcgtt
     3781 gggagttttc atttgatccg gggccatcgt gcagatagcg gggcgggagt tccgacggct
     3841 gcggctgggg aacagggtct gtgttcgatc gtcgcagtgc tgagaaaacg cgatgtgagg
     3901 ttgacgttta cggcgaggcg gcagctttca tgctgcgctg aagcccgcag cgctacattt
     3961 aaaccttcaa ggcgcggcca gggcaccgtc gtctccagca ggcttgactt cgcagggcgc
     4021 gtgcggcaga gtttgcggcg ttgctatgga tcgcagaact tttttgaggg cttcggcggg
     4081 ggcggcggct gcggcagatg gcgtcccaat ctcgacgaat ttctattgcc attcatccag
     4141 aacaggcact tggggaaact ccggcggatt ttcttggact tgggtacgag atttcttccg
     4201 ttgcgaggcc ggggttgctc agcgcgcgca atacggctta cgttcagttt gtgaggaatc
     4261 ttggcaggcg cggagtgatc cgcatcggag gcaatacttc ggatttttca agttacgcga
     4321 aggacgggcc ggcagtttgc gcgaccaagg gcaccgtggt gaacgatgcc gtgatccgcg
     4381 acttggggga atttctcgac gcgaccggtt ggcaactgat ctgggggctg aatcttggca
     4441 gccgcgatct cgcaaacgct atcgagcaag caacgagcgt gatcgcgggt acgaaggaca
     4501 agctgcttgc gttcgagatt ggtaacgagc ccgatctttt tgcgggtgga gtcgcacacc
     4561 ggcccaaggg atattcgtat gcggattatc tgaaggaata tcgcgaatac aaaacggcgc
     4621 tgcgagcgaa attccccaaa agcccttttg ccgggccgga tgtggcaagt cacaccgatt
     4681 ggctgacgca atttgcagcg gacgaagggt cggatctgaa gctgctgacg catcatcact
     4741 acgccgaagg gcctccgacg agtccggcaa gcacgatcga gaggcttttg actccgtccg
     4801 aaggcttgac gcgcatgctg aatcagtgcg aggcggcttc gcgtagcgcg ggcgtgccgt
     4861 acagaatttg cgagacgaat tcttgttttg gcggcg
//
LOCUS       DATNDT010000132         6963 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3740850, whole genome shotgun sequence.
ACCESSION   DATNDT010000132 DATNDT010000000
VERSION     DATNDT010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3740850"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..3081)
                     /locus_tag="VN788_04160"
     CDS             complement(97..3081)
                     /locus_tag="VN788_04160"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19759.1"
                     /translation="MNAPGQESTELFLQEASENLQYLREYSGLLQEPQTKVEDLERLY
                     IASHTLAGTSASYGFPQFSEVAAKMAHIFHYAMNAALTPDMHGPLTEFLSDAISVLEF
                     DLLQISSSGSETSSDIAAFKQRYAFAFPAAPTADTSGDERVESVGEGEPIPTIFPAAS
                     LADTLPQDGEVPDEILEFFIPEAEEHLQAVTECLLALEGHPNADDINRLFRSMHTVKG
                     SAAQVGLHRLSAVAHRVEDLIGRLRDGALQPSTEIVDLFLQSVDVLKKFLHRQWQTDA
                     EMVAAVDPLLTSIAELAPEEVAEESGRDSLVLETASSSEISEAPAEQLSSAPRPVAAQ
                     ALPQAKSVRISLEWLDRMMNAVGELVINRTRMLGRLSELSKLVEVLQFSKARLTGKVS
                     DFQEKHEFSRVRSTLVPGSQAPQMDTFGGHRALPGVTSNDLLDFSELEMDRYDDFNIL
                     SRSLTEISADVTEVLTQLEGFMGRVDSDIDEFTKLAHHLQDEITAARMVPIGNLYTRL
                     SRTVRDAAKASGKPVDLSLQGADTELDNNIVQHISDPLIHLVRNAIAHGIEDGELRSR
                     AGKPEKGRITVRAYHRGNHIFIEVEDDGRGIDYEAIRRSLVESATISSIAAAELSERE
                     LREFLFRPGFSTASSTSELAGRGVGLDVVRANVHALNGEIEVRSEPGRGACFTVKVPL
                     TLIISQALFVRCGTSVFAFPLAFVEEIRRLRPAEIEDVGGKLLTRVRDVVTEVVRLDL
                     QLALSPLEQLNGYFHMVIVKVAGRQVGVVVEEVLGKDEIVIKNLGDYLRRVKLFPGTT
                     IAPDGSLILLIDLNRLTAADTAERHALPASSPAARVFAPGAEAVASGSIPSEAVDAIE
                     NDRVVVVADDSISVRKFVGRMLEKAGYRVKLASDGLEASEIVAQVGCHLLITDLEMPR
                     MNGYELMAHLRQDPVTRRIPVLVVTSRAGAKHRDRALKEGALGFLTKPVQEDQLVAAV
                     ENLIGSEAPQGRPLVGVPSRQD"
     gene            complement(3078..3962)
                     /locus_tag="VN788_04165"
     CDS             complement(3078..3962)
                     /locus_tag="VN788_04165"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXU19760.1"
                     /translation="MKPHPVTTARLTESELDQIRTLIEHRSAILLDSSRERFFSTRLR
                     EYLEEKGLASGTDLLAHVRGSSIEYEALLERLLTQETSFFRYPAVFEALEKRILPEVQ
                     ERKFWENPRTLRIWSAGCSTGEEPYSVAITVADALKFAEAWEIEILATDISRRALRHA
                     ERGVYSKRSLQNVSLGQIEAHFTNSKAGFQVKPRIRRMISFAQMNLVESVYVGKIDCI
                     FCMNVLMYFSEERRLGILRRFYDALEPGGYFLLGHSETLINVPVKFEPVVLGDCRIYR
                     KPGPVEVRRAPVLAEGAQ"
     gene            complement(4012..5466)
                     /locus_tag="VN788_04170"
     CDS             complement(4012..5466)
                     /locus_tag="VN788_04170"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HXU19761.1"
                     /translation="MKARLSSTLWFLVLWAGVALLGVLALAYHAGKESGGIALLDASS
                     LTGAAGGALLGALALALIAALSLAFVLSKRVIKPVGELAKFSERLAAGDGRSRGELTS
                     NDEFGFIVENFNRTAAKIAHAATNQQAQDSLQRSITDLLNTINLVARGDLTIRGKVTN
                     DALGNVVDSVNFMLDNFTKVLERVRKAAIDVSASANQILDATDDMTAGATQQDQEITN
                     TSSAVEELTVSMKQVSNNAEASAEAARRALDAAEQGNRAVSDTLEGMQRIRASVQATA
                     KKIKSLGDRSLEISEIINVINDITEQTNLLALNAAIEAARAGEAGRGFAVVADEVRKL
                     AEHSRSATKDIAALIKAIQAETNEAVVVMEEGTREVEVGAGLADQAGKALEAISSVVR
                     QSAELVQEISLASKQQVRGTEGVANAMQIISGITRQTTQGARQTASTVGNMVKLSEQL
                     NEALAQFRSQSTRAIESSTTAEEARPAMPVGAHR"
     gene            complement(5583..6080)
                     /locus_tag="VN788_04175"
     CDS             complement(5583..6080)
                     /locus_tag="VN788_04175"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXU19762.1"
                     /translation="MPDLEPGAFLESESAQEERRPERQYCVFRAGRERFCFSVLEVEE
                     VVDWPIVTRIPLAPSFLMGIFNLRGTIVPLMDIAFTEGRRPGLLPKHVVVAALSGDAL
                     REDLRLGIAADEVIGTYSATGDSLLDQAPAEVPHCRGMLRHEDRLALIVDLRRLTDVF
                     PVPVI"
     gene            complement(6110..6472)
                     /locus_tag="VN788_04180"
     CDS             complement(6110..6472)
                     /locus_tag="VN788_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19763.1"
                     /translation="MIKKVLVVDDSQAEIRLLQSVLQQAGFHSVGISDPTRIEETIEE
                     ERPNLILLDVVMPRRNGFQACRDLKNQEPYSKIPVILVTSKTAPSDRYWGEQQGANGF
                     VAKPFTSDELISTVKRFA"
     gene            complement(6607..>6963)
                     /locus_tag="VN788_04185"
     CDS             complement(6607..>6963)
                     /locus_tag="VN788_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19764.1"
                     /translation="LVSIEALRRVEATNRAQRAENHSQACQRHAVLGRYMLEMRPSVN
                     NALTSVLGNADLLLLEPGQNSGESREQIRAIQVMALRLNEIMQRFSSLASEMRSDEKE
                     SQAETQTASNRIVAVP"
BASE COUNT         1454 a         1962 c         2095 g         1452 t
ORIGIN      
        1 ggatcggagt tgataatttc tttcaggacc ttgcacatga aaggcgaatc atccaccacg
       61 aggacgcgta tttttttact gatggctgcg ttcatctcaa tcctgccgcg aagggacgcc
      121 aactagcggc cgcccctgcg gcgcctccga gccaattaaa ttttcgaccg cggcaaccag
      181 ctggtcctct tgcacaggct tggtgagaaa gcccagagcg ccttccttca atgcgcggtc
      241 gcgatgcttc gcgccggcgc gggaagttac gacgagaacg ggaatgcgcc gggtaacggg
      301 atcctgccgc aagtgggcca tcagctcgta gccgttcatg cggggcattt cgaggtccgt
      361 gataagcaga tggcagccaa cttgcgcgac gatttcagag gcttccagtc catccgaggc
      421 aagcttcact cgatagccgg ctttttcgag catgcgtcca acgaatttgc gcacgctgat
      481 ggaatcgtcg gcgactacta ccacacggtc gttttcaatc gcatcgaccg cttcggaggg
      541 gatgcttccg gaagcgaccg cttcggcacc gggcgcgaag acacgggccg ccggagaaga
      601 tgcgggcaga gcgtggcgct cggccgtgtc cgccgcggtc aggcgattca gatcaatgag
      661 aaggatcaag ctgccgtcgg gggcaatggt cgtgcccggg aagagcttaa cgcggcgcag
      721 gtaatcaccg agatttttga tgacgatttc gtccttaccg agaacttcct ccaccacaac
      781 gcccacttgc ctgccggcga cttttacgat caccatgtga aaatagccgt tcagctgctc
      841 gaggggtgac aatgcgagct gcagatcgag gcgcacaact tcggtgacca cgtcgcgcac
      901 acgcgtaaga agcttcccgc caacatcctc gatttccgcg gggcgaagcc gtcgaatttc
      961 ctcgacgaaa gcgagcggaa atgcgaagac cgaagtgccg cagcggacaa acagggcctg
     1021 ggaaataatc agcgtcagag ggaccttgac ggtaaagcaa gcgccgcgac cgggctcgga
     1081 gcgaacttca atttcgccat tgagcgcgtg cacgttggcg cgcacgacgt cgaggccaac
     1141 gccacggccg gccagttcgg atgtggagga ggccgtcgag aagccgggac gaaacaggaa
     1201 ttcacgcagt tcgcgctcgg aaagctctgc ggcagcgatg gacgatatcg tcgcagattc
     1261 gaccaggctg cggcggattg cttcgtagtc gatgccgcgg ccatcgtctt ccacctcgat
     1321 gaagatgtga ttgccgcggt ggtaggcgcg cacggtgatg cggcctttct ccggcttccc
     1381 ggcgcggctg cgcagttcgc cgtcctcgat tccgtgcgcg atggcattgc ggacaagatg
     1441 aatcagagga tcggagatgt gctgaacgat attgttgtcc aactcggtat cagcaccttg
     1501 gagagagagg tccacgggct tgccagaggc tttggcggcg tcacgaacgg ttcgcgaaag
     1561 gcgcgtgtag agattaccga tcgggaccat gcgcgcagcg gtgatctcgt cctgcaggtg
     1621 atgcgcgagc ttggtaaatt catcgatatc ggaatcgacg cggcccatga aaccttcgag
     1681 ctgagtgagc acttcagtga cgtccgcaga aatttcggtg agggagcgcg acaagatgtt
     1741 gaagtcgtcg taacgatcca tttcgagttc gctgaaatca agaagatcgt tggaagtgac
     1801 gcccggtagc gcgcgatgcc ccccgaacgt atccatttgc ggggcttgac tgcctggcac
     1861 gagcgtagag cgaacgcggc tgaattcgtg cttttcctgg aagtcggaaa ctttcccagt
     1921 taggcgtgcc ttcgagaact gcagcacttc gacgagtttt gaaagctcgg acagacgacc
     1981 gagcatgcgg gtccggttga tgaccagttc gccgaccgcg ttcatcattc tgtccagcca
     2041 ttcgagcgaa atgcggacgg actttgcctg cggaagggcc tgcgccgcaa cgggacgggg
     2101 tgccgaggaa agctgctcgg cgggtgcctc agagatttcg gaggatgatg ctgtttcgag
     2161 caccaacgaa tcgcggcccg attcctcagc tacctcctcg ggcgccagct cggcgatgct
     2221 cgtgaggagc ggatcaactg ccgccaccat ttcggcatca gtttgccact gccgatgcag
     2281 aaatttcttc aggacgtcga cagactggag aaacaaatcg acgatctcgg tactcggttg
     2341 gagcgcgcca tcgcggagcc gtccgattaa atcttccacg cgatgggcca ctgcggatag
     2401 ccgatggaga ccgacctgcg cggccgaacc ttttacggtg tgcatcgaac ggaagaggcg
     2461 attgatatcg tccgcgttgg gatggccttc gagggccagc aggcactcgg ttactgcctg
     2521 caagtgttct tcagcctcgg gaatgaaaaa ttcaaggatc tcatcgggca cttcaccatc
     2581 ttgaggcagc gtgtccgcga gagaagcggc ggggaaaatc gtcgggatgg gctcaccttc
     2641 gcccacagat tcgacgcgct catccccgga tgtgtcagcg gtcggcgccg ccggaaacgc
     2701 gaacgcgtag cgctgcttga atgccgcgat gtcgctcgaa gtctccgaac cgctggaact
     2761 gatctgcaag agatcgaact cgagcacgga aatcgcgtct gaaaggaatt cggtgagcgg
     2821 gccgtgcata tccggagtta gtgcggcatt catggcgtag tggaatatgt gcgccatctt
     2881 ggccgcaact tccgagaatt gcgggaatcc gtaacttgct gaagtaccgg caagcgtgtg
     2941 cgacgcaatg tatagacgtt cgagatcctc gactttcgtt tgaggttctt gcaaaagacc
     3001 ggagtattcc cttagatact ggaggttttc ggaggcctcc tgcaggaaca gttccgtgga
     3061 ttcttggcca ggcgcgttca ctgggctccc tccgccaaaa cgggcgcgcg gcgcacttcc
     3121 acgggacccg gtttgcgata aatccggcag tctccaagga cgaccggctc gaatttaact
     3181 ggtacgttta tgagggtttc ggaatggccc agcaggaaat atccgccggg ctccagcgcg
     3241 tcatagaagc ggcgaagaat ccccaggcgg cgctcctcgg agaagtacat caacacgttc
     3301 atgcaaaaga tgcagtcgat tttgccgaca tacacggatt cgacaagatt catctgcgcg
     3361 aaggagatca ttcggcgaat gcgtggcttt acctgaaatc cggccttgga attggtgaaa
     3421 tgggcttcga tctggccgag tgagacattc tggaggctgc gcttggaata gacgccgcgt
     3481 tcggcgtgcc gcaaggcgcg gcggctgata tccgtggcga gaatttcgat ctcccaggct
     3541 tccgcgaatt tgagcgcgtc ggcgactgtg atcgccacgg aataaggttc ctcgccggtg
     3601 gaacagcctg cgctccaaat gcgcagcgtt cggggatttt cccaaaactt tctttcctgc
     3661 acttcgggca ggatgcgttt ctcgagcgct tcaaaaacag ctggatagcg gaagaaggag
     3721 gtctcttggg taagtagccg ctcgagcagc gcttcgtatt cgatactcga gccgcgcacg
     3781 tgcgccagca gatcggttcc gctggccaaa cccttttctt cgagatattc ccgcagacga
     3841 gtggaaaaaa atcgctcgcg agaagaatcg agcaaaattg ccgagcggtg ttcgatcagg
     3901 gtgcgaatct ggtcgagctc ggactccgtg agcctggcgg tcgtgactgg gtgaggtttc
     3961 atgttcggcg ttcagctccg gctgcaatgg accggtaggc ggaatgtgcc gtcagcgatg
     4021 cgcccccact ggcatggcgg ggcgcgcttc ttccgcagtc gtcgaggatt caatggctcg
     4081 agtggactgg gaacggaatt gcgccagcgc ttcatttagc tgctcggaaa gtttgaccat
     4141 gtttccgacg gtcgaagcgg tctgacgcgc gccttgggtg gtctggcgag tgatgccgga
     4201 aatgatttgc atggcgttcg ccacgccttc cgtgccgcgc acttgctgct tcgaagcgag
     4261 tgaaatttcc tgaacgagtt cggcggattg ccgcacaacg ctggaaatcg cctcgagtgc
     4321 cttaccggct tgatcggcga ggccggctcc cacctcaact tcccgggtac cctcttccat
     4381 gacgactacc gcctcattcg tttccgcctg aatggccttg ataagcgctg cgatatcctt
     4441 tgtagcgcta cgcgagtgtt cggccagttt gcggacttcg tcagcgacca cggcgaaacc
     4501 acggccagct tcacccgcac gggctgcttc gatggcggcg ttcagcgcga gcaggttggt
     4561 ctgctcggtg atgtcgttaa tgacgttgat gatttcggag atttcgagcg aacggtcgcc
     4621 gagagattta atcttcttgg cggtggcttg cacggaggca cggatgcgct gcatgccttc
     4681 gagcgtgtca ctcacggcgc ggttaccctg ttccgcggca tcgagagcgc ggcgcgctgc
     4741 ttcggcgctt gcttccgcgt tgttggaaac ctgcttcatc gagaccgtca gttcttctac
     4801 ggcggacgag gtgttggtga tttcctgatc ctgttgagtg gccccggccg tcatgtcgtc
     4861 cgtggcatcg agaatctggt tggcgctggc gctgacgtcg attgcggcct tgcggacgcg
     4921 ttcgagaacc ttggtgaagt tgtcgagcat gaagttgacg gaatcgacga cgttgccgag
     4981 ggcgtcgttg gtcaccttgc cgcggatggt gaggtcgcca cgagcaacca gattaatagt
     5041 gttgagcaag tcagtaatgc tgcgctggag cgagtcctgg gcttgctggt tggtggccgc
     5101 gtgcgcgatt ttggcggcag tgcggttaaa attttcgacg atgaatccaa attcatcatt
     5161 gctcgtaagc tcgccacgag acctgccatc acccgccgcg agccgttccg aaaactttgc
     5221 cagttcgcca accggcttga tcacgcgctt gctcagcaca aaagcgaggg acagcgccgc
     5281 aatcagcgct agagcgagcg cgccaagcaa ggctccgccg gcggctccgg tcagtgaaga
     5341 cgcgtcaagc agagctattc ccccggattc ttttccggcg tgataggcga gggcgagcac
     5401 gcccaaaagg gctactccgg cccagagaac gagaaaccaa agcgtggaac tcaaacgagc
     5461 tttcatgact gcctcccaag acttgcgcgg ccatcgccgt gaacaatatg ccgtagccga
     5521 tttcgaccgg accgccttaa aaatcgcgaa ttaacggcct cgcaaaacat tgccgagcct
     5581 tttcaaatta ccggaaccgg gaaaacgtcc gtcaagcgcc gcaggtcgac gatcaacgcc
     5641 aggcgatcct cgtgccgcaa cattccgcgg cagtgcggca cttcggccgg cgcctgatcg
     5701 agcaacgaat ccccggttgc tgaatacgtt ccgatcactt cgtctgccgc gatgccaaga
     5761 cgaaggtctt cacgaagggc atcaccgctg agcgcggcaa caaccacatg tttgggcagc
     5821 aatccaggcc gacgaccttc agtaaaagcg atatccatca acggtacgat cgttccgcga
     5881 agattgaaga tgcccatcag gaacgacggc gccagcggaa tgcgcgtaac gattggccag
     5941 tccacaactt cctcgacctc cagcaccgag aagcaaaatc gctcgcggcc ggcccgaaaa
     6001 acgcagtatt gccgttccgg gcgccgctcc tcctgagccg attcgctctc tagaaaggcg
     6061 cccggttcaa ggtcgggcat gacccccaca ttttccggcg catccgaaat caagcgaacc
     6121 tcttcacagt gcttatcaat tcatccgagg tgaaaggctt agccacgaag ccgttggcac
     6181 cttgttgctc gccccagtac cggtcgcttg gcgcagtctt cgacgtgacg agaatcacgg
     6241 ggattttcga atacggttct tggttcttca aatcgcgaca ggcctgaaag ccgttgcgcc
     6301 gcggcatcac gacgtcgagc aagatcaggt tgggacgttc ttcctcaata gtttcctcaa
     6361 ttcgggtagg atccgaaatc ccgaccgagt gaaatcccgc ctgctgcagg acggattgca
     6421 ataatcggat ttccgcttgt gaatcatcga ccactagaac cttcttaatc accgccgcac
     6481 ctccggagtt aatttgcccc gagagaagga ttgcaagtcc tcggccaccc ttcgaagagg
     6541 aatcactgca gacttgctct tggtaagcat cctgattgca gcggcttaga cattcagact
     6601 gccaagtcag ggcacggcca caatccggtt ggaagccgtt tgagtctcag cctgagattc
     6661 tttctcatcg ctccgcatct cgctagccag cgatgagaat cgctgcatga tttcattgag
     6721 ccgcaaagcc ataacttgaa tcgcacggat ttgctcgcgg ctttctccgg agttctggcc
     6781 tggctccagg agcaggagat cggcattgcc gagtacggat gtcagagcat tatttacgct
     6841 tggtcgcatc tcaagcatgt aacggccgag gactgcgtgc cgctggcaag cttgagagtg
     6901 gttttcggca cgttgtgcac ggtttgttgc ttcgacgcgc cggagcgctt caatcgaaac
     6961 gag
//
LOCUS       DATNDT010000133         9372 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1665078, whole genome shotgun sequence.
ACCESSION   DATNDT010000133 DATNDT010000000
VERSION     DATNDT010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9372
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1665078"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..448)
                     /locus_tag="VN788_04190"
     CDS             complement(<1..448)
                     /locus_tag="VN788_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXU19765.1"
                     /translation="MKITSAAFAVAALLGVGAVVSALNAQENGKQPDALVHVLPVQAN
                     IYMLVGAGANITVQIGDTGVLLVDTGLPAMSDQILAAIRTLSDKPIRFIIDTTDDPDH
                     IGGNEEIAKAGSTITGGNVVGDIGASAGNTAAIIAFQTILDRMSAPT"
     gene            complement(445..1332)
                     /locus_tag="VN788_04195"
     CDS             complement(445..1332)
                     /locus_tag="VN788_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19766.1"
                     /translation="MSNAERFKVLSLSLLLLVLASGPALAQNDPSGEWANRFHEDEPE
                     RIPGPEIGDYLGLPINEAARLRGDSWDATLLELPENQCRPHPSDYAWRGPALLRIWKE
                     VDQQTQKLIAYHTHISWQSPEQTIYMDGRPHPPAYAPHTWQGFSTGKWQGDILEVTTD
                     HLKEGWIRRNGIPRSDRATVTTYIMRHDRYLTVAVLISDPAYLTEPFIRTTDFVSAPQ
                     QQIGPYPCESVDEVDRPIGAVPNHLPGTNTFLDEFPAAYGIPPQAARGGAETMYPEYR
                     SKMKTMTTLPRPTKKHTDR"
     gene            1667..3253
                     /locus_tag="VN788_04200"
     CDS             1667..3253
                     /locus_tag="VN788_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007576557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXU19767.1"
                     /translation="MVSNEKSQIANASGTTRAQPFTSREFMDSLRDDREIWIYGERVK
                     DVTTHPAFRNTVRMMARLYDALHDKRKGVLCTETDTGSGGYTHKFFKASRNVEEMVGA
                     RDAIAEWARVTYGWMGRSPDYKAAFLATLGANSEFYTPFQENARRWYKKVQEEVTFIN
                     HAIVNPPVDRNKELAEVRDVYMHVEKETDGGLIVSGAKVVATTSTLTNYNFIANNGAL
                     PIKTKDFAFVCIVPTNAKGLKLFCRPSYEMTAAVMGSPFDYPLSSRLDENDSILVFDK
                     VFIPWEDVFAYGDIEKVNNFFPRSGFLPRFMFHGCVRLAVKLDFIAGLLLKSVDATGA
                     KDFRGVQAQVGEVLAWRNLFWGLTDAMARTTTPWTPGYVLPNLDYGLAYRVMASMAYP
                     RLKEIIENVLASALIYLPSHANDFKQPEIRKHLDQYVRGSNGYSAVDRVKLMKLMWDA
                     MGTEFGGRHELYERNYFGNHESIRFEVLMVADSMGQSAKYKGFAERCMAEYDLDGWTV
                     PDLINPDDISAVMKSGSLKR"
     gene            3302..3832
                     /locus_tag="VN788_04205"
     CDS             3302..3832
                     /locus_tag="VN788_04205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXU19768.1"
                     /translation="MQFRNALGRFASGVTVVTVRYEEHTHGMTANAFVSVSLDPPLVL
                     VSLDNRSYMHKILPRVARYGISVLAENQQALSNHFAGHTVEGLRIHFTTRKEIPLLEG
                     AVAYFVVQVIDAHPAGDHSLYIGRVEHFECRDDRPLLFYAGSYQQLGVQKAKPTPWPQ
                     DEFSLFSIGSVDPPIT"
     gene            5065..5430
                     /locus_tag="VN788_04210"
     CDS             5065..5430
                     /locus_tag="VN788_04210"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXU19769.1"
                     /translation="MPRLRSFSPKRLSKIEEERTEAATLIVGRNPVYPRIAKDHLISG
                     NVEVRFRINPEGKIYGVESVKGAPILAAAAIDAVKAWRYRPARQNGVPIDSQASTNFD
                     FSLDPDSSDCSVVQITSQK"
     gene            5440..6726
                     /locus_tag="VN788_04215"
     CDS             5440..6726
                     /locus_tag="VN788_04215"
                     /inference="COORDINATES: protein motif:HMM:NF021375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU19770.1"
                     /translation="MYQSYFGLQENPFNVTTDPRYLYVTQRTQEALNCLTYGIEARKG
                     FILLTGEVGTGKTTLLNKLLDTLHQQRVATAFVFNPRMPAAQLFDYMMADFGIECKSK
                     KRSHVLFKLHQWLLDRYQAGEQTVLVVDEAQILSRQALEEVRLLTNLETSTEKLLQIV
                     LAGQPEIEQKLNQRELRQLRQRIALRAKTVPLTVEETCGYILERLRIAGANGEEIFSP
                     LAIDAVHRHARGIQRVTNLLCEQALLRAWVSQRRPIPPEIIDEVARDFSLDEVELLAQ
                     GFPHERPSSHGNRHRNGRKRGSSKRRGSRTASESRSEQSGHEPPLGVTQARLPLFVYG
                     YGADGNLFYEDAYTVAMSARGGLISMNTSVKPGQRLILTNKENECSQECVVEFLGARL
                     ARGVDVGFEFPTPRPDFWSIREIDQESIGEGEEACA"
     gene            6775..8703
                     /locus_tag="VN788_04220"
     CDS             6775..8703
                     /locus_tag="VN788_04220"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXU19771.1"
                     /translation="MYEDFFRMNESPFQLSPDPFFLFRTKKTNEVLASIAHAINLRKG
                     FVVLTGEVGTGKTLVLRCLFELWEGERIPFAYFIGPKLSTVDFLSYITFELGINVKEP
                     TKGKLLRALYGFLLTQFDKGLTTVLVIDEAHEMPRNVLEEIRVLANFETAQQKLIQIV
                     LVGQPELEKKLDLVELRSLKQRIAVRCQLEPLSAEEIRHYIDRRLQLAGAPLEAGAIF
                     PSRTIEAIHRYSMGIPRLVNSICDQALMAAFARQIHTVQVDIIDEVATHFRLDHNPHL
                     STRTEKPSSVARQIETSNQNKSRQASPTANASGMKAGDLETPPTPMAVSEATPAQMAT
                     SVESETSNHRKLNDKETAGSKFNSRFMRAIRGPQLGPWLEPGLRLSILVIAAALLPMA
                     LAAGFFVARHQKELTMTPRQVMSMRDTPLGPEAGTIEPVGVGSTKQTDIGSNPIPRQR
                     ESRDLKSPAELDHSTSRMKMVMSKLSRPVRKSFNLSLPAPPPTVVMQTKQLDVGKGLL
                     DLSVPSPPPPQAITTGQVRQMKPAPSFPSATILGKGAARLTPNAGDGLQPAKLVLSRP
                     PAYPAIARAASVAGLVVIDAVVDETGKVTDMKVISGHPLLTGAARDALHAWKYDPARL
                     DGRPVVAHVQVRMNFRLP"
     gene            complement(9179..>9372)
                     /locus_tag="VN788_04225"
     CDS             complement(9179..>9372)
                     /locus_tag="VN788_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19772.1"
                     /translation="SFGTDSHGPTQLRFIAFAAASALQSGIKRDRILNFMSREELLAW
                     VGDVRELARKFERKNGLFR"
BASE COUNT         2217 a         2527 c         2571 g         2057 t
ORIGIN      
        1 ccgtgggcgc gctcattcga tcaaggatgg tttgaaacgc aataatggct gccgtgtttc
       61 cggcactggc gccgatgtcg cccacgacgt tgcccccggt tatggtgctg ccagccttcg
      121 caatctcctc attccccccg atgtgatcgg gatcgtcggt ggtgtcgata atgaagcgaa
      181 tcggtttgtc cgagagggtg cggatcgccg ccaagatctg gtcgctcatt gcagggagtc
      241 cggtatcaac gagcagcacg ccagtgtcgc caatctggac agtgatgttg gctcccgcac
      301 cgaccagcat atagatgttg gcctgtaccg gcaggacatg gacgagcgcg tcgggttgct
      361 taccgttctc ctgcgcgttc agcgccgaaa cgacggcgcc gactcctagc agagcggcga
      421 ctgcgaacgc ggctgaggtg attttcatcg gtccgtatgt ttcttggtcg gacgcggcag
      481 cgtggtcatg gtcttcattt tcgatcggta ctccggatac atcgtctccg caccgcctct
      541 tgcagcttgg gggggaattc cgtacgcggc gggaaactcg tcgagaaatg tgtttgtccc
      601 tggcaaatgg tttggcacag ccccgatcgg gcgatccacc tcgtccaccg attcgcacgg
      661 atacggcccg atttgctgct gcggtgcgct gacaaaatcg gtcgtgcgaa tgaaaggttc
      721 tgtcaggtac gccgggtcgg aaataaggac ggcgacagta aggtaacgat cgtgccgcat
      781 gatatatgtg gtcacagtgg ctctgtcgct gcggggaatg ccatttctcc ggatccagcc
      841 ttccttgagg tggtcggtgg tcacctcaag aatgtcccct tgccactttc ccgtagagaa
      901 gccctgccag gtgtgcggcg cataggctgg cgggtgcggg cggccgtcca tgtaaatagt
      961 ttgttcggga gactgccacg aaatgtgcgt gtggtaggcg atgagcttct gagtttgctg
     1021 atctacttcc ttccagatcc tgagcaaagc gggcccacgc cacgcatagt cggaagggtg
     1081 aggtctgcac tgattttccg gaagttccag aagcgtagcg tcccagctat cgcctctgag
     1141 gcgcgcggcc tcattaatcg gcaagccgag gtagtcccca atctccggcc cggggatgcg
     1201 ctcaggctcg tcctcatgga acctatttgc ccattccccg ctcggatcgt tttgggcgag
     1261 tgccgggccg ctcgccagta cgagcagcag caacgaaagg ctcagaacct tgaatcgctc
     1321 ggcgttactc attcctcact cctgggtgcc gctggccatg ggcaaacaaa gtggaggcaa
     1381 ggggaccgat tcagcgttgc cccttctttg aggggccatg accaatcctg ggataccaaa
     1441 ggcccggtca atttgcttgc agaaactcca ctggaatgga cagattcgtg accgtgcgag
     1501 cgaaggcaat aaggtgccat ttttagcatc cggaagggtt ggaatcgaga gtcatgattc
     1561 tgctcacgat agcggagttc ctgcaagccg gagctttcga ggtgctttag cctgaatctt
     1621 gagaggaatt gccgaaaaga ccgaaagtca ttgagggagg attgcgatgg ttagtaatga
     1681 aaagagccag atcgcgaacg caagcggtac cacacgggcg caaccattca cgtcgcgcga
     1741 attcatggac agtctccgcg acgatcgcga gatttggatt tacggtgaac gagtaaagga
     1801 tgtcacaacg cacccggcct tccgcaatac cgtgcgtatg atggcgcgcc tctatgacgc
     1861 gctgcacgat aaacgaaaag gtgtgctctg caccgagact gacacgggca gtggcggcta
     1921 cacccacaaa tttttcaaag catcgcgcaa cgtcgaagaa atggtcggag cgcgcgatgc
     1981 catagcggag tgggcacgag taacgtacgg ctggatgggt cgaagccccg actacaaagc
     2041 cgcatttctc gcaaccctgg gcgcgaactc tgagttctac actccgtttc aggagaacgc
     2101 gcggcggtgg tacaagaaag tgcaggaaga agtcacgttt ataaaccacg cgatcgtcaa
     2161 tccacccgta gatcgcaata aggaactcgc cgaagtgcgc gacgtttaca tgcacgtcga
     2221 aaaggaaacc gacggtgggc tgatcgtcag cggcgcgaag gttgtggcta cgacttccac
     2281 gcttacgaac tacaatttta ttgcgaacaa tggcgcgctt ccgatcaaga ccaaagattt
     2341 cgctttcgtc tgtatcgtcc caacaaacgc aaaaggtcta aagctcttct gccggccatc
     2401 atatgagatg accgcagccg tgatgggcag tccgttcgac tacccgctat cgagccgtct
     2461 cgacgagaac gactcgatcc tcgtgttcga taaggttttc ataccatggg aagatgtctt
     2521 tgcgtatggc gacatcgaaa aggtgaacaa tttctttccc cgctctggtt tccttccgcg
     2581 attcatgttt cacggttgcg tgcgcctggc ggtgaagctc gatttcattg ccggcctctt
     2641 gctgaagtcg gtcgatgcta cgggcgcaaa agattttcgt ggtgtgcagg cgcaggtggg
     2701 agaagtgctg gcttggcgca atctgttctg gggcctgacc gatgccatgg cccgcacaac
     2761 tactccatgg acgcctggtt acgtactgcc gaatcttgat tatgggttgg cttatcgcgt
     2821 gatggcgagc atggcttatc cgcgcctcaa agaaatcatc gaaaacgtgc ttgctagcgc
     2881 gctcatctat ctcccatctc acgccaatga tttcaaacag ccggaaattc gcaagcatct
     2941 cgatcaatat gtgcgcggct caaacggtta ttcggcggtc gatcgagtga agttgatgaa
     3001 gctgatgtgg gacgcgatgg ggacggaatt tggcggccga catgagcttt acgaacgcaa
     3061 ctacttcggc aatcacgaaa gcatccgctt tgaagtcctg atggtcgctg attcgatggg
     3121 acaatccgcg aaatacaagg gcttcgccga acggtgcatg gccgagtacg atctggatgg
     3181 ctggactgtg ccagatttga tcaatccaga tgacattagt gcggtgatga agagcgggag
     3241 cctgaagcga tagacgaaat agaggagaag ttcgatcgaa cgcggtcagc ctttcgaccc
     3301 cattcagttc cggaacgcgc tagggcgttt tgccagcggt gtaaccgtgg ttaccgtgcg
     3361 ttacgaggaa catacgcacg gtatgactgc caatgcgttt gtgtccgtgt cgctcgatcc
     3421 gccgcttgtg ctcgtttctt tggacaatcg ctcgtacatg cacaaaatac tgccccgcgt
     3481 tgcgcgctac ggcattagtg tactggcgga aaatcaacag gcgttgagca atcactttgc
     3541 cgggcatacg gtcgaaggcc tgcgaattca cttcaccact cgaaaggaaa ttcctctgct
     3601 ggaaggcgcg gtcgcgtatt tcgttgtgca ggtaatcgac gcccatccgg ccggagacca
     3661 ctcgctctac atcggccgcg ttgagcactt tgaatgtcgg gacgacaggc cattgctatt
     3721 ttacgccgga agctatcagc agttgggcgt ccagaaagca aagccgacac cctggccaca
     3781 ggacgagttt tctctattct caatcggcag cgtcgatccg ccaatcactt agcctcgagt
     3841 accctgccca cgctccgaat ctgtcgcact caagacatgg cttgctgccg ataaatggtc
     3901 gacgaaactt cgacaagcgg ccaaaaccca ttggaacgat ggctgcgcaa agaaaaaccc
     3961 caacatggcg attcaggcta gctggctatc tgtgctcgtc agcagatctc gacttttgaa
     4021 tacagcgccg aagcaggtac tggtgggaag agtagtgcga gcttgcgtag ctaacagagc
     4081 gatgcgaaag gtaaaaatcg cctacaattc atgagcaagg cagcgccatg ctcaatcacc
     4141 ctagtgcgac gtactgaaaa taaggaagac atttggtcgg ggcgtggaga tttgaactcc
     4201 agacctcctg cgcccaagac aagagtgcca ccagcgaaga ggtctcccta atcgaatcag
     4261 ttggttgtga cctccgaccg aaatggcgtt acaggattcc acggggtcat tttgtcggtc
     4321 acaacggtcg ttagggtcaa tttggagaac cgatggcagg caaacgcctc ggccgccagc
     4381 gcagcgaatt tccttggggc agatctaatt tctcggaaag tcgcgttaat tgggacctga
     4441 gctcgttatg cgaacgagat tcctccagat cagccgtggg agttcaattg aagcgtcctc
     4501 cgagtggggc tttgcatatt cgctgggctt gtctttgccc tgctcgacat ctcgcaccga
     4561 ctcagaagga gcaatcaatt acttggcatt gagatcagca accctttacc tgacccgaag
     4621 aagaggtttc ctgaaacatt tccacaatcg cgcggttttc cacagcattg atccattgct
     4681 tatctgattg acgccagtcc tagcaagcct agtatgtgcc tcagaaaaat tcgattcagc
     4741 ggtcaattga agctatcgcg gcaactctag cagaagtagt gtgtgtggag acgtcaggtg
     4801 agcatcgttt tcgacgcccg tcggttcgcc gaacagtcaa aacgcgggcg gggagtgagt
     4861 tctggcattc cgcaatcgcc tgactccgaa cgatgttcgc cggagtcgca tggcggtgcg
     4921 ccaagtaagg accacgtaag cctccaagac gaaccggtcc gcgggaatgc acgtttagcg
     4981 tgttccgacg gttcatggac gagtgacata agcgccgcca cggaagacgc cgcgcaaaca
     5041 cctctcgtgc gaaccctatt gcggatgcct cgtctacgct cgttttctcc aaagcgtctg
     5101 tcgaagatcg aagaggaacg gaccgaggca gccaccctaa ttgtgggccg aaatccggtc
     5161 tatccgagaa tcgccaagga tcaccttatt tctggaaacg tagaagtgcg ttttcgcatt
     5221 aatccggaag gcaagatcta cggggtcgaa tcggtaaagg gggcacctat cttggccgcg
     5281 gccgccatag acgccgtaaa ggcgtggcgc tacaggcctg cgcgtcaaaa cggggtacct
     5341 atcgattcac aagccagcac gaactttgat ttcagtctgg acccggattc gagcgattgt
     5401 tcagttgttc aaataaccag ccaaaagtga ggtactgaga tgtatcaaag ctatttcgga
     5461 ctccaagaga atcctttcaa cgtaactacc gacccgcgtt acctgtacgt tacgcagcgc
     5521 acacaggagg ctctaaactg cctgacgtat gggatcgaag cacgaaaggg ctttatcctc
     5581 ttaaccggcg aggtaggaac cggcaaaacc acactgttaa ataagctgct cgacacctta
     5641 catcagcagc gcgtcgccac ggcgtttgta tttaatccgc ggatgccggc agcgcaactc
     5701 ttcgactata tgatggcgga ctttggtatc gagtgcaaat ccaagaagag gagccatgtt
     5761 ttgttcaaat tgcaccaatg gctgctcgac cgctatcagg ccggagaaca gaccgttcta
     5821 gttgtcgatg aggctcagat tctctcccga caggcactgg aagaggttcg gctgttgacg
     5881 aacttagaga cttcgaccga gaaactcctc cagatcgtac tcgccgggca accagagatc
     5941 gaacagaaac ttaatcaacg ggagcttcgt cagcttcgcc agcgtattgc ccttcgcgca
     6001 aaaactgttc cactcacggt ggaagaaacg tgtggataca ttctcgagcg cttgcgcatc
     6061 gccggtgcca atggagaaga aatttttagt cctcttgcta ttgacgcggt gcaccgacat
     6121 gcacgtggaa tccagcgcgt aactaatctc ctttgcgagc aagcgttgct tagagcctgg
     6181 gtcagccagc ggagacctat tccaccggaa atcatcgacg aggttgcacg cgattttagc
     6241 ctagatgaag ttgaactgtt ggcacaaggt ttcccacacg agcggccaag ctcgcatggt
     6301 aatagacaca ggaacgggcg taagcgcggg tcttcaaaac gtcgtggatc cagaaccgca
     6361 agcgaaagcc gatcggagca atccggccac gagccgccat taggggtcac tcaggcaagg
     6421 cttccactgt ttgtctatgg ctacggggcg gatggcaact tgttctacga ggatgcctac
     6481 actgtcgcaa tgagtgcgcg cggcggattg atttccatga acacgtcggt gaagcctggc
     6541 cagcgcctaa tcctaacgaa caaggaaaat gaatgctcac aagaatgcgt ggtcgaattt
     6601 ctgggggcac gccttgcgcg aggtgtggat gtgggttttg agtttcccac tccgaggcct
     6661 gacttctgga gtatccggga aatagaccag gaatcaattg gagaaggcga agaggcttgc
     6721 gcatagctaa aaaacttgtt cgcaagcgtc acgcagcgac cagattggaa ccttatgtac
     6781 gaagactttt ttcgcatgaa tgagagtcca tttcagctct cacccgatcc gttctttttg
     6841 tttcgaacga aaaagaccaa cgaggttctg gcctcgattg cacatgccat aaacctgcgc
     6901 aagggcttcg tagtcctgac cggcgaagta ggaaccggca agacgctggt actgcgctgt
     6961 ctatttgagt tgtgggaggg tgaacgaatc cctttcgctt actttatcgg cccaaaactg
     7021 tcgaccgttg attttttgag ctacataact ttcgagctag gaattaatgt caaagaaccg
     7081 acgaagggaa agctgctacg cgcactatac ggatttcttt taacgcaatt cgataagggc
     7141 cttacgactg tgctggtaat cgacgaagca cacgaaatgc cacggaacgt gctggaagag
     7201 atcagggtgc tggccaactt cgaaactgct caacaaaagc tcattcagat tgtcctagtg
     7261 ggacaacccg agcttgaaaa gaaattggat ctagttgagc tgcgttcgtt gaagcagcgc
     7321 attgccgtcc gttgccagtt agaaccgctc tccgcagaag aaattcgcca ttacattgac
     7381 cggcggctgc aactcgcggg cgctcctttg gaagcaggcg caattttccc gtccaggaca
     7441 attgaagcga ttcaccgata ttcgatgggc atcccaagat tagttaacag catttgtgac
     7501 caggccttaa tggctgcctt cgcgcggcag attcacactg ttcaggttga cattatcgat
     7561 gaggtagcaa cccattttcg acttgaccac aatccacacc tttcaacccg aaccgaaaag
     7621 ccttcctcag ttgcgaggca aatcgaaacc tccaaccaga ataaatctcg gcaggcctca
     7681 ccaaccgcga atgcatcagg tatgaaagct ggcgatctgg aaacgccgcc aacaccgatg
     7741 gccgtgagcg aagcaacccc tgcgcaaatg gcgacgtcag tcgaatcaga aacatcgaat
     7801 caccgcaagc taaatgacaa ggaaacggcc ggttcaaaat tcaattcaag gtttatgcgt
     7861 gctatcaggg ggccccaact aggcccctgg ttagagcccg ggcttcgatt atcgatcctc
     7921 gttatagcgg ctgcgttgtt gcccatggcc cttgcagccg gtttcttcgt ggcccgtcat
     7981 caaaaggaac taacgatgac accccgacag gtcatgagca tgcgagacac gcctcttggg
     8041 ccagaagctg gaacgataga acctgtcgga gtaggatcaa ccaagcaaac cgacattggt
     8101 tcgaacccca tcccacgtca gcgcgagagc cgggacttaa agtcacctgc tgagctcgat
     8161 cattcgacat ctcgcatgaa aatggtaatg agcaagttgt cgaggcctgt tcgtaaatca
     8221 tttaacctgt cgcttcctgc gcccccaccg accgtggtga tgcagacgaa gcagttggac
     8281 gttggaaagg ggctgcttga cctttccgtg ccctctccgc caccaccgca ggcaattacc
     8341 accggccagg tgcggcaaat gaagccagcg ccgtcatttc catcagcgac tatattgggg
     8401 aagggcgctg cgcgactcac cccaaacgct ggtgacggac tgcaaccggc gaaattagtg
     8461 ttgtcacgcc cgccagccta cccggccatc gcgcgtgcag cgagtgtcgc aggcctagtg
     8521 gtaatcgacg cagtcgttga tgaaaccggt aaagtcactg acatgaaagt gatttcagga
     8581 cacccccttc tgacaggggc ggcgagggac gctcttcacg catggaagta cgatcccgcg
     8641 cggttggatg gccgacctgt cgtggctcac gtacaagtca ggatgaattt taggttgcct
     8701 tgagtttcgg cgggcacgca ctccggcgac ctcgtttccg cattcaaggt ccgatgtcgt
     8761 gccctttaca caccaccggc tccagagcaa ggtcggaatt tcgttggtac accacttaat
     8821 aaccggaaaa cgaatagcct cgcgtcctcc gcacccgatc gtgaggccta tagcagtacg
     8881 ctaagcatgc cggacccgca tcgtccctca tcccataggc atgttcacta gacgggcgaa
     8941 cccacgcgtc gttgtgaccg acttggcgca ccccttggag taaaattttg agaaagaata
     9001 aggtaatttc cacccctttc aaacgccatc gtgcgcaaag gtgtccattc attgctggag
     9061 ttcaaataac cgctctcgca ttacattctt tctttcccca ccgcaatgcg aggacggaag
     9121 cctcgatctt acgtcccgcc gttttcctga taacagactt cgactcagca cacagtcttc
     9181 atctgaacaa accattcttt ctttcgaact tacgtgccag ttcgcgcacg tcaccgaccc
     9241 aagccaggag ctcttcgcgc gacatgaagt ttaggatgcg gtcacgcttg attccagact
     9301 gaagagccga tgctgcggca aatgctataa accgaagctg tgtgggccca tgagagtccg
     9361 tcccaaacga ta
//
LOCUS       DATNDT010000134         7037 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2351871, whole genome shotgun sequence.
ACCESSION   DATNDT010000134 DATNDT010000000
VERSION     DATNDT010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2351871"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(511..894)
                     /locus_tag="VN788_04230"
     CDS             complement(511..894)
                     /locus_tag="VN788_04230"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HXU19773.1"
                     /translation="MDRIGEFLGKALRRMERPEAAMAWLSNAWPTIVGKALAEHAHPL
                     KCQSGCLELAADGKPWQQQLENMKREICFRINQAWGGNLVSEVKFPSSGDSSGVRNSL
                     STPGPARASLEADNEHIPFIRRRRA"
     gene            complement(1021..1575)
                     /locus_tag="VN788_04235"
     CDS             complement(1021..1575)
                     /locus_tag="VN788_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19774.1"
                     /translation="MGRYGEALDEIHRSLELDPLSAVSNYMEGDIFFSQRDYRGAIAQ
                     EEKTLEIDPNFALAYSVLRDCYDAQGMYDNAVNAYERLFTLNGTPEVAEELKQVYESS
                     GIRGAREWWIQQRSDRAGRRYSPYTVAINYLGLGDKDKTFLWLEKAYEHRDIGFIGFK
                     DPVFDSVRSDPRYIDLLRRISSPQ"
     gene            1899..3137
                     /locus_tag="VN788_04240"
     CDS             1899..3137
                     /locus_tag="VN788_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007221409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19775.1"
                     /translation="MATEVNWGIYRDTLRMALRALKRNKLRTSLTMLGIMIAIAAVIC
                     TVAVGQGATRQVQDQLTALGTNMIWIEAGGRNVNGVRTGNGATKTLTVDDAAAIKASV
                     PQIASVAPNVDGPMQVVYGNQNWYTRFRGVSSDYFEIRNWPQASGTLFTSMDSQHETN
                     VCLLGKTVAKLLFGSDNPIGKTIRLQNLPFKVIGTMEEKGFSPTGFDQDDIVVIPYTT
                     AMKKLKGIYWLDDIYASAVSQEAITPAIDEITAVLRERHHLRPDEPDDFNIRHPEETL
                     QAQEEASRTLTLMLAGIASVSLLVGGIGIMNIMLVSVTERTREIGVCLAVGATETDVQ
                     KQFLAEAVTVSLLGGAAGVLFGVGASAFISRILQWPSLISPLAIMVATMLSVAVGVLF
                     GYYPARLASRLDPIEAIRYE"
     gene            3300..3374
                     /locus_tag="VN788_04245"
     tRNA            3300..3374
                     /locus_tag="VN788_04245"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3333..3335,aa:Glu,seq:ctc)
     gene            3507..4043
                     /locus_tag="VN788_04250"
     CDS             3507..4043
                     /locus_tag="VN788_04250"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXU19776.1"
                     /translation="MPSRISCPDNHATKGISIHEAAIAISAGRAIGICKKCGKELQYR
                     IDHAYPGDPNERLHSFTVTRAIRLGTGLIGNEGYDPFLLVLRQIETGKERILPTFWVD
                     GQTAQRGGQFPPLLTFEEWKTLFRRLDATFDEMEERIRVRAYELYEKGGRRDGSALAD
                     WLQAEAELAEQVILGIAA"
     gene            complement(4048..5148)
                     /locus_tag="VN788_04255"
     CDS             complement(4048..5148)
                     /locus_tag="VN788_04255"
                     /EC_number="1.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016346817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018506 - maleylacetate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetate reductase"
                     /protein_id="HXU19777.1"
                     /translation="MTADSLAPFTYEAAPLRVVFGLGSLEKAPEEIDRLGAKRVLILT
                     TPGRKKLGEELAWRLGPKAAGVFGAAVMHVPAETAEAGRAEAARLQADCYIAIGGGSA
                     TGLAKAIAVETGNPILAIPTTYSGSEMTPTWGLTDHGIKKTMRDTRARPKTAIYDPAL
                     TTSMPTMLSATSGMNAIAHCVEALYSQEASPIVSLIAEEGIRALTKSLPTVVKEPSNM
                     EARSLALYGAWLGGTSLGAVSMALHHKLCHVLGGAFNLPHSETHTVLLPHATAYNAVA
                     APEAMERIARALGAKDGANSAAQGLYDLEAAMGAPLSLESIGMKREGLEHAAELPVQK
                     PFYNPRKLSKDEIRTLLEDAFEGRPPRSWTAP"
     gene            complement(5377..6198)
                     /locus_tag="VN788_04260"
     CDS             complement(5377..6198)
                     /locus_tag="VN788_04260"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXU19778.1"
                     /translation="MNRPVVLVSARRTARKNKLIDYVGELHLELLIRLRILPVIVPVV
                     AGTEACLPQYMESMDGLLLVEGEDIEPKRFPARKANFEYLEKTHPLKDEIEIRLLRFA
                     LRRHLPILGICRGSQLLNVVCGGTLYGDVQKEKHSHRPHINYHNYDTYRHPLSIVPGT
                     PLAQWYRRKTLEVNSYHHQGIRELARRFRPMAYADDGLIEAYYDSRPAFVVGLQFHPE
                     RMLKEYAGNWRVWKSFAAAVHRGHRSRLEMAPKAGRRLHRTRKSSISSKSTGAHA"
     gene            complement(6195..>7037)
                     /locus_tag="VN788_04265"
     CDS             complement(6195..>7037)
                     /locus_tag="VN788_04265"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXU19779.1"
                     /translation="GNGATELIALIDVALIDRMAVPIPTFGEYIEKMKDQRDAELYAL
                     EPESGYTLRLEDYLAWVHQRKLESLLIINPGNPTGQFIPLEEMVEFLQQARDLELVIV
                     DESFIDFAGDPIPSLLPLADRFTNLLIVRSMSKHCGVPGLRLGYCYSANLFVLNRLRR
                     FVPTWNLNTLAQYFLSLLPPTDAAYHEGRKRLIADARWLYGNLRTIPRLQVYPTGANF
                     VLFKIQNAMTATELQARLLAEHQMYVRDCSNKLGMDAFHIRVASQGYEKDARLVEALR
                     TLMR"
BASE COUNT         1463 a         1825 c         2183 g         1566 t
ORIGIN      
        1 gatgtcgatt tgcttgagct tatcgatgta gctgaggatg ccatcgagct ggcctctgta
       61 ggtgtccact tcctcgggag acaaaccaag atgggccagt tcggcgattc tcagcacttc
      121 ttcgcgacta attttcatgg cggatttgat ttctcggcgg cgtgcgtttc gaaaaagagc
      181 cttctcggtt ccgtgacggg taggcgcggc accggccgtt tgttttcatg gttttacatc
      241 actttctact cggctcgtgt gtcgattagg attcttcgcg tgagcggtct tgccgaggac
      301 cggcctgaaa tggtcgggtc tccctgcggg gtgcgctcgc ttcgcgctcc caggcgtcaa
      361 tgtcaggcga cgcgacgcgg ccagccggct gctgccggct tctcgacaga tgcaatcgta
      421 aatcatggga attatcttag aggaaacgca tggcaatgca agcggcagcg cctgtggact
      481 tttttttgga cgggttttgc aggctatttg tcaggcgcgg cgtctgcgaa tgaaggggat
      541 gtgctcgttg tcggcttcaa gggacgcgcg ggctggaccg ggcgtcgata gggaattccg
      601 aacgccggag gaatcgccgc tggaagggaa cttcacctca ctgaccagat ttccgcccca
      661 ggcttggttg atgcggaagc agatttcgcg cttcatgttt tcaagttgct gctgccacgg
      721 ctttccgtcc gcggctaatt ccaagcagcc gctttggcat ttcagggggt gggcgtgttc
      781 cgcgagggct ttgccaacga tcgtgggcca ggcgtttgaa agccacgcca tcgccgcttc
      841 gggccgctcc atccggcgga gcgccttgcc cagaaattct cctattcgat ccatgagggt
      901 cgcgattcta acacagagga caaccgagag tttgcgtcga ggctcgggtc gggtgaaaca
      961 gatccctcac ccccatccgc gaacagcgcg gctggattcg ggacgacatc gcagtatcgg
     1021 tcactgcggg gagctgatgc ggcggagcag gtcgatgtaa cgcgggtcgg agcgcacgct
     1081 atcaaatacc ggatccttga agcctatgaa tccgatatcc cgatgctcgt aggccttttc
     1141 cagccagagg aaggttttgt ccttgtctcc cagaccgaga tagttgattg ccaccgtgta
     1201 agggctatac cttcggccag cgcgatcgct tcgctgttgg atccaccact cgcgcgcgcc
     1261 cctgattcca gaggactcat aaacctgttt caattcttct gccacttcgg gtgtaccatt
     1321 caaggtgaag agccgctcgt acgcattgac agcgttgtcg tacatgcctt gggcgtcgta
     1381 gcaatcacgt aaaacggagt acgccagcgc aaaattcgga tcgatctcca aagttttctc
     1441 ttcctgggca atggccccgc ggtagtcgcg ctggctgaaa aagatgtcgc cctccatata
     1501 gttcgagaca gctgacaagg ggtccaattc aagagagcgg tggatctcgt ccagcgcttc
     1561 gccgtatcgg cccacggttc tcaggtaggc tccattgagc tcatgcgcaa tccggaagtt
     1621 tggatttagt ttccgggcgc gttgaatttc agatccggcg ggtccgaatt ccagggaaag
     1681 ccagtggatt ccgcccatag acgcgtgtgc cgccaccgag ccttgcaacg atgcggtagt
     1741 gcgagagccg ttggttttcg gtgaggtctg cgccgggcat ggtggaggga ttctaagctg
     1801 ctttgctgct tcctcggagc aaatttgtgg aatgggacgt gagcgagcgt cgcagcacgc
     1861 agccggggta aaatgcggga cggagtcgac atattcgaat ggcaaccgag gtcaactggg
     1921 gaatctatcg ggatacgttg cgcatggcgc tgcgcgcgct gaaacgaaat aaactacgca
     1981 cgtcactaac gatgctcgga atcatgattg ccattgcagc ggtgatctgc actgttgctg
     2041 tggggcaagg ggcgacacgg caagtacaag atcaactaac ggcgcttggc actaatatga
     2101 tctggatcga agcgggcggg cgcaatgtga acggggttcg aaccggcaac ggagcaacga
     2161 agacgcttac tgttgatgac gccgcggcaa tcaaggcgag cgttccacaa atagcgagcg
     2221 tggctccgaa cgtcgatggg ccgatgcagg ttgtgtacgg aaatcaaaac tggtacacgc
     2281 gttttcgcgg tgtctcatca gattatttcg agatacggaa ctggccgcaa gcgagcggaa
     2341 cgctttttac ttcgatggac tcgcaacacg aaactaacgt gtgccttctg gggaaaacgg
     2401 tggcgaagct gctcttcggt tccgataatc cgattggaaa aacgatccgc ctgcagaatc
     2461 tgcccttcaa ggtgatcggc acgatggaag agaaaggctt ttcgccgacg ggtttcgatc
     2521 aggatgacat cgtggtaatc ccctacacta cagccatgaa aaagctgaag ggtatctatt
     2581 ggctcgacga tatttatgcg tcggcagttt cgcaggaggc gataacgcct gccattgacg
     2641 aaattactgc cgtgctacga gagcgacatc acctgcggcc cgacgagccc gacgacttta
     2701 atattcgcca cccggaagaa acgcttcagg cccaggaaga agcgagccgg acgttgacgt
     2761 tgatgctggc gggaattgct tctgtctcgt tgttggtggg aggcatcggc attatgaaca
     2821 tcatgcttgt ctccgtgacg gaaagaacgc gtgagattgg cgtgtgcctt gcggtcggcg
     2881 ccacggaaac cgatgttcaa aagcaatttc tggcggaagc ggtgacggtg agtttgctcg
     2941 gcggagcggc gggagtgttg ttcggagttg gcgcatccgc attcatctcc agaatattgc
     3001 aatggccctc gctgatttcg cccttggcga taatggtcgc cacgatgctt tcggtcgcgg
     3061 taggagttct ctttggttac taccctgctc gcctggcctc tcgcctcgac ccgattgagg
     3121 ctattcgcta cgaataggta ttttctcttg taagagaatt ttcagagcag agcgcgcgga
     3181 gttgccggtc ggttgcgcgg ggaattcagg catgcgaaaa ttgcctgcat cgtttatctg
     3241 caagattctt ctccgcaaat ttgcgaggct ttttgcgatt gcgaatggga atctgccggg
     3301 gcgatatggt ctaagggtta ggacgcgacc ctctcaaggt cgaaatccgg gttcgaatcc
     3361 cggtatcgct accaatcgaa gtgccatcgg atctgcgaag atcacctaaa aaaaaccgca
     3421 ggaagcatgc gccgcgcgtg aagtttagat gtaaactttt tgtattccac ggcggccgac
     3481 ggatagaccg ccaagaggtt gaatttatgc cgtctcgaat aagttgtccc gataaccatg
     3541 ccacgaaggg aatctcgatt cacgaggccg ccattgccat ttcagcaggg cgcgcaattg
     3601 gcatctgcaa gaaatgcgga aaggaactgc agtacagaat cgaccatgcg taccccggcg
     3661 atccaaatga aaggctccac agctttacgg tgacgcgggc gattcgattg gggactgggc
     3721 taataggcaa cgaaggctat gatcctttcc ttttagttct ccgccagatc gaaacgggta
     3781 aagagcggat tcttccgacc ttttgggttg atgggcaaac cgctcaacgc ggtggacaat
     3841 ttccgccgct tctgacgttt gaggaatgga agacgctctt tcggcggctg gatgccacgt
     3901 tcgacgaaat ggaagaaaga attcgagtga gggcctacga gctttacgaa aagggcggga
     3961 ggcgcgacgg ttcggccctg gcagactggc tgcaagcgga agcagagcta gcggagcaag
     4021 tgattctcgg cattgccgcg tagctggcta cggcgccgtc caggaacgcg gcggacgccc
     4081 ctcgaaagca tcttccaaca gcgtgcgaat ttcgtccttc gatagcttgc gcggattgta
     4141 aaaaggcttt tggacaggaa gctcggcggc gtgttccaga ccttcgcgct tcattccgat
     4201 ggattcgagc gacagcggcg cgcccatcgc tgcttccaaa tcgtagagtc cctgcgcggc
     4261 ggaatttgcg ccgtcctttg cgccgagcgc gcgcgcgata cgttccattg cttcgggagc
     4321 ggcgacggca ttgtaggctg tggcatgcgg gagcaagacg gtgtgcgttt cggagtgtgg
     4381 caagttgaag gcgccgccga gaacgtggca cagcttgtga tgcagcgcca tggaaaccgc
     4441 gccgagcgag gtcccaccaa gccacgcgcc gtagagggcg agcgatcgcg cttccatatt
     4501 tgacggttcc ttcactacag taggcagact tttcgtcagc gcgcgaattc cttcttcagc
     4561 gatcagcgac acgatggggc tcgcctcctg cgaatagagc gcttcgacac agtgagcaat
     4621 ggcgttcatg ccgctggtgg ctgaaagcat ggtgggcatg ctggtggtga gcgcgggatc
     4681 gtagatcgct gtttttgggc gggcgcgcgt gtcgcgcatc gtttttttga tgccgtggtc
     4741 ggtgaggccc caggtgggag tcatctccga accggaatag gtggtgggga tggcgaggat
     4801 tggattgccg gtttccacgg cgattgcttt cgcgagcccg gtggcagagc cgcccccaat
     4861 cgcgatgtag caatcggctt gcaggcgcgc ggcctcggcg cgtcctgctt cggcggtttc
     4921 cgcggggacg tgcatcacgg cggcaccgaa aactccggct gcttttgggc cgaggcgcca
     4981 ggccaattct tctccgagtt ttttgcggcc gggcgtggtc aggatcagaa cgcgcttggc
     5041 gccgaggcgg tcgatttcct cgggcgcctt ttcgaggctg ccgaggccga acactacgcg
     5101 caatggcgcg gcttcgtaag tgaagggcgc taaggaatcg gcagtcatgc ccgaaccact
     5161 cgggaaagtg tcgagtagcg cgtacgtcgg tcttttgttt tgtaagagtt acaggcgatt
     5221 ctactcgact taactgtcga gcagaaatcc tgcaaagcgt ggtgcgcagg atggagggct
     5281 agtgttcgag gcacttggcc tcccgtgccg ccgaactgcg cgttgagtgc ggtcttttgg
     5341 accatgtgag catgttgttg gagagggacg gtcgtgtcaa gcgtgggcgc ctgtggactt
     5401 cgaggaaatt gagcttttgc gggtacgatg gaggcggcgt ccagctttgg gcgccatttc
     5461 caaccgactg cgatgaccgc ggtgcacggc cgctgcgaac gacttccaga ccctccaatt
     5521 cccggcgtat tccttcagca tgcgctcggg gtgaaattga aggcccacta caaaagcagg
     5581 tcgggaatcg tagtaagcct cgatcaatcc gtcatcggca tatgccatag gccggaagcg
     5641 gcgcgctaat tcgcgaattc cctggtgatg atagctgttt acctccagcg tttttcggcg
     5701 ataccactgc gcgagagggg ttcccggaac tatggaaagc ggatggcggt atgtatcgta
     5761 attgtgatag ttgatgtggg gacgatgcga atgtttttcc ttttgtacgt ctccatagag
     5821 agtccctccg caaaccacat tcaataactg cgacccgcgg cagatgccca gaatcgggag
     5881 gtggcggcgc aaggcgaagc gcaggagccg aatctcgatt tcgtccttga ggggatgcgt
     5941 tttttcaaga tattcgaaat tggctttcct ggctgggaag cgcttgggtt cgatgtcttc
     6001 accttcgacg agcagaagac cgtccatcga ttccatgtac tgtggaaggc acgcctctgt
     6061 gccggcgacg acagggacga ttaccggcag gatgcgcaag cggatgagaa gctcaagatg
     6121 aagctcgcca acgtaatcga tgagcttgtt cttccgagcg gttcggcggg cgctgacgag
     6181 aacaaccggc cggttcatcg catcagtgtc cgcagcgctt ccaccagtcg agcgtccttc
     6241 tcgtaccctt gcgaggcgac acgaatgtga aacgcatcca tgccgagttt attggagcaa
     6301 tcgcgcacgt acatttgatg ttcagccagc aggcgcgcct ggagttcggt ggcagtcatt
     6361 gcgttttgta ttttgaagag aacgaaattt gcgcctgtgg gataaacctg aagacggggg
     6421 attgttcgca ggttgccata gagccagcga gcgtcggcga tcaagcgctt gcggccctcg
     6481 tgataagcgg cgtcagtggg cggcaaaaga gacaggaaat attgcgcaag ggtattcaaa
     6541 ttccaagtgg gaacgaagcg gcgcagacgg ttgagtacaa aaagattcgc gctgtagcaa
     6601 tatcccagcc ggaggccggg cacgccacag tgcttgctca tgcttcgcac gatcaggaga
     6661 ttcgtgaacc ggtcggcaag cggaagaaga ctgggaatcg ggtcaccggc gaaatcgatg
     6721 aaagattcat cgacgatgac cagctccaaa tcccgcgcct gctgcagaaa ttccaccatc
     6781 tcttcgagcg ggatgaactg gccagtagga ttgccgggat taatgatcag cagggactcg
     6841 agttttcgct ggtgcaccca ggccaagtag tcttcaaggc gcagcgtgta gccgctctcg
     6901 ggttcaagcg catagagctc ggcatcccgc tggtccttca tcttttcgat gtattcgcca
     6961 aaggtaggga tgggcaccgc catgcggtcg atcaaggcca cgtcgattag agcgatcagt
     7021 tcggtggcgc cgttgcc
//
LOCUS       DATNDT010000135         6170 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4136023, whole genome shotgun sequence.
ACCESSION   DATNDT010000135 DATNDT010000000
VERSION     DATNDT010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6170
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4136023"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1675
                     /locus_tag="VN788_04270"
     CDS             <1..1675
                     /locus_tag="VN788_04270"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HXU19780.1"
                     /translation="QILNQMIFQRALEYEAGQMGLRVTPEEEAQRIKEILPDAWSGNT
                     WLKDRYTTEVQTRTGMDVSEFESLLRDQMLVDKFRQLVTDGVTVSQPEIEQEFRWRNE
                     KVTIAYALIKPADLASSIHPGDSELSAWFAKNKSRYQIPEKRSARYVLLDLAKLRAST
                     TVSDDVLRDYYKSHIDEYKVENRAHVEHILFKTVGKTDAEIAEIHQKAEDVLKQAKHG
                     ANFEDLAKKYSEDDGTKAKGGDLGWIVDGQTVPEFQQAAFSLPKGSISDLVKTQYGFH
                     IIKVLDRETAHTKTFEEVRDSILQTVLDQKVTTEADDLSNQMAQAVRQSDRQSLDDLA
                     KKFHLQVGDTPAASMTDAIVPLGNSPELHQTLFALHVGELSQPIQIDSGFVIITPKNI
                     SPAHQATLAEAHDQVLADYQHEKSVDLARSRSEELLKRVRAGEDFDKVASSLGLTVKK
                     SDPFARNGSIPDVGTSRQLAAAFTLPVGKTGNPIQSGENWVVFRTLTHEVPDPADLAK
                     QATDLRQELLQTKQNAAFEAFHTGLVNRLTQEGKLTINTDAVDRMTRSS"
     gene            1935..2537
                     /locus_tag="VN788_04275"
     CDS             1935..2537
                     /locus_tag="VN788_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19781.1"
                     /translation="MPIKSFTPGRLAVVVRGDRLIRVGRGADRAAASYQKKLAFINQG
                     NGSRLNEYHCAVERTQNRKYNVRLRAIFGSSPEGRDSAGSAKPRPAGWTLSAYFLASS
                     FNAAMKQLALSLQLLQKNEDRLRFWGVERTDDPNVVGDLLREFGLWLDRRKEFPRKIA
                     EFRVPRERAISAASLAPVRRILADSIAERRPIYRTELAGD"
     gene            complement(2592..2873)
                     /locus_tag="VN788_04280"
     CDS             complement(2592..2873)
                     /locus_tag="VN788_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19782.1"
                     /translation="MAAEKMVMVGVLPEAIVIDPGEQVVWFSNAGNIKIEFDSQRSPF
                     SSNIFQAPPGVRLSSGATRPGLNPGSYRYRLSLNDVVVAHGEVVLRGKQ"
     gene            complement(2937..4811)
                     /locus_tag="VN788_04285"
     CDS             complement(2937..4811)
                     /locus_tag="VN788_04285"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HXU19783.1"
                     /translation="MSATTQAAPSASAVETIKEKARRLSLLSMMETTAAGSGHPTSCM
                     SAAELMAGIFFYAMKFDPKNPNSPDADRFVLSKGHAAPVLYAALAEAGVFPDSRVMTL
                     RQFSSELEGHPTPRIPGVDAATGSLGQGLSVGAGLAIGARLDKSPTRVYVLLGDGEMA
                     EGQIWEAAEFAGHYKLDNLTVIADVNALGQSDPTMYQHDMEIYRQKFAAEGFAAEVID
                     GHDVPAVLAALDRAKGTKGRPQAILARTIKGHGFSEVAGKEHWHGKPFSKEQLARAEK
                     ELGGAIEVPPDPGKSYARSSLPKPPDFPAPAAPDYEAGKPVATREAYGFALKRLGEVN
                     PHIVAASGDVKNSTFSEIFQKAFPDHFFQGYIAEQNLVSLGVGLAARGKVPFLDTFAC
                     FLSRAYDNVRMAAISRANINLCGSHCGVSIGEDGPSQMALEDIAMFRAVHGSAVLYPS
                     DAMSAERLTETMARRGGINYLRTSRPKTAILYSKEEKFPVPGFKVPRKSAQDRVTVIG
                     AGITVHEALKAADELKSQGTAIRVIDLYCVKPLDGKALAAEISATGGRVVTVEDHWAE
                     GGIGEAVLSALAQAGTAPSKFRLLAVREMPHSGKPDELVAAFGISARHIAEAVREIAS
                     "
     gene            5023..5601
                     /locus_tag="VN788_04290"
     CDS             5023..5601
                     /locus_tag="VN788_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018602295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXU19784.1"
                     /translation="MYPRSVPRRKEPIHPPTFTIGHSTRPIDDFIALLRANGIRQLID
                     IRTIPKSRHNPQFNGDALAKSLRAARIQYLHMKDLGGLRHAKPDSINLGWRNASFRGY
                     ADYMQTPEFEAALERAINLAQKKPTALMCAEAVPWRCHRSLVADALLARGIPVLEIVS
                     RAGPKEHKLTPFARVRGTKVTYPSDQQSLLPI"
     gene            complement(5625..>6170)
                     /locus_tag="VN788_04295"
     CDS             complement(5625..>6170)
                     /locus_tag="VN788_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HXU19785.1"
                     /translation="YAATSVGLYHMATNLSLAMDFGTAAMLRTYQVAGLAFIFIPSNV
                     LCYAGIPREKNNQVSSMMNFVRNIGGSIGIAVVSTMVTRGTQTRQVYLSANMQNGNPR
                     FRAMVDGIAASLRSGGISAAESTHQAYVRIAMMMQQQASALAFRDVVAILAVIVLCLV
                     PVAFIMQRPAKAAPGAVPPPH"
BASE COUNT         1355 a         1908 c         1640 g         1267 t
ORIGIN      
        1 gcagatcctc aatcagatga ttttccagcg ggccctcgaa tacgaagctg gccagatggg
       61 ccttcgtgtc acccccgagg aggaagctca gcgcattaag gaaattctgc cggacgcttg
      121 gtcgggaaac acgtggctca aagaccgcta caccactgaa gtgcagactc ggacggggat
      181 ggatgtgtcg gaatttgaga gccttcttcg agaccagatg cttgtggata aattccggca
      241 gctcgtaacc gacggcgtta ctgtcagcca gcccgaaatc gaacaggaat ttcgctggcg
      301 caatgaaaaa gtcaccattg cctacgcgct gatcaagcct gccgatctgg catcgtcaat
      361 ccaccccggt gattccgagc tttcggcctg gttcgccaaa aacaagtcgc ggtaccagat
      421 accggaaaag cgttccgctc ggtacgtcct gctcgatctc gcgaagcttc gcgccagcac
      481 caccgtcagc gacgatgtgc tgcgtgacta ctacaaatcg catattgacg aatacaaagt
      541 cgaaaatcgc gctcacgtcg agcatattct cttcaaaacc gtcggcaaaa ccgacgccga
      601 aattgccgaa atccaccaga aggccgaaga cgttctgaag caagcgaagc acggtgcgaa
      661 tttcgaggat ttggcgaaga agtattccga ggacgatggc acgaaggcaa aggggggcga
      721 tctgggctgg atcgtcgatg gccaaactgt tcccgaattc caacaggcag cgtttagtct
      781 cccgaagggc tcgatatcgg acttggtgaa aacccagtac ggctttcaca tcatcaaggt
      841 cctcgaccgc gaaacggcgc ataccaaaac ctttgaggaa gtgcgggatt ccatccttca
      901 gaccgtgctc gatcagaagg taactaccga agcggacgac ctttcaaacc agatggccca
      961 agccgtccgt cagtccgacc gccagtctct tgacgatctc gcgaaaaagt tccatctcca
     1021 ggtaggtgat acgccggcgg cctccatgac ggacgcgatc gtgccgctcg gcaattctcc
     1081 cgaattgcac cagacgcttt ttgctctcca cgtcggcgag ttgagccagc ccattcagat
     1141 tgatagcggc ttcgtgatta tcactccgaa gaatatttcc ccggcgcacc aagccaccct
     1201 cgccgaagcc cacgaccagg ttctggcaga ttatcagcac gaaaagtccg ttgatctggc
     1261 ccgttcccgt tccgaagagc tcttaaagcg agtgcgagcg ggcgaagatt ttgacaaggt
     1321 agctagctcc ttaggcctaa cagtgaaaaa gtccgatcct ttcgctagga acggttccat
     1381 tcccgatgtt ggcaccagcc ggcagctcgc ggcggccttc actctccccg tcgggaagac
     1441 tggcaatcca attcaaagcg gcgaaaactg ggtagtcttc cgcaccttga cgcatgaagt
     1501 ccccgatcct gccgatcttg ccaaacaagc aaccgatctt cggcaggaac ttctccaaac
     1561 gaagcagaac gccgccttcg aagcattcca caccggcctc gttaatcgtt tgactcaaga
     1621 aggcaagctc actatcaata ccgacgctgt tgaccgcatg acgcgttcaa gctaattcct
     1681 catttcgcgc tggctcatca ttcgattttt gagccttctt acaaaatgac gttgccccaa
     1741 gagctccttg tatcctattg taaataggat acttacttgt tagtttgttg ggcttgtcgg
     1801 gccgcaccgg gccgcgatag aatactgttc ttcgtggagc ctgccccaag tcagcggggt
     1861 tcggtctcta cccgaagccg tcccggtctg gacgcgtggc aaccgagcag cggcttcttc
     1921 ttaggagtat ttatatgcct atcaagagtt ttaccccagg aagactcgca gttgttgtgc
     1981 gcggggatcg ccttatccgt gtaggacgcg gagccgacag ggcagcagct tcctatcaga
     2041 aaaagctcgc attcatcaac caggggaacg gttctcggct caacgagtat cactgcgccg
     2101 tcgagcgcac tcagaatcgt aagtacaatg tgcgtctgcg agccatattt ggctcaagcc
     2161 ccgaaggacg cgattccgca ggttctgcga agcctcgccc agcgggatgg actctctcgg
     2221 cctatttcct ggcctcatct ttcaacgcgg cgatgaaaca gctcgctctg tctctgcaac
     2281 tccttcagaa gaacgaagat cgcctccgtt tctggggcgt ggagcgtacc gacgatccca
     2341 atgttgtcgg cgaccttctc cgggaattcg gcctctggct tgaccgtcga aaggaatttc
     2401 cccgcaagat tgctgaattc agagtgcctc gcgagcgcgc gatttcggct gcctcgctgg
     2461 cgcccgtccg gcgaatcctg gcggactcga tagctgagag acgcccgatt tatcgcacgg
     2521 aactcgcagg cgactgaagg caattgaagt cctgccctga cggtaaagac gccggaccgc
     2581 aatcaaaacc gttactgctt gcctcgaagc accacctcgc cgtgggccac cactacatcg
     2641 ttgagggaga gccggtagcg atacgaaccg gggttcaggc cggggcgcgt cgctccgctg
     2701 gatagacgga cgcccggcgg cgcctgaaaa atgttcgagg aaaatgggga tcgctgcgag
     2761 tcgaattcaa tctttatatt gccggcattt gaaaaccata caacttgctc cccgggatcg
     2821 atgacgatcg cctcgggaag cacacccacc atcaccattt tttcagcagc cactcaacgc
     2881 gtcctttcgc ccgcctgaag gcggccgctg caaccagttg tagctggcgc taaagtctat
     2941 gaggcgattt cgcggacagc ttccgctata tgccgcgcgg agatcccgaa cgcagccacc
     3001 agttcatcgg gctttccgga atgaggcatc tctctaaccg caagcaaccg gaacttcgaa
     3061 ggtgccgtac ccgcctgggc cagcgcggaa aggacggctt cgccaattcc accctccgcc
     3121 caatgatctt ccaccgttac aacacgtccg ccagtggcac taatctccgc cgcaagcgct
     3181 ttcccatcaa gcggtttcac gcaatataaa tcgattacgc gaatcgccgt gccctgcgac
     3241 ttcagctcgt cggccgcttt cagcgcctcg tgaaccgtga tgcccgctcc aatcaccgtc
     3301 actcggtcct gcgcgctttt acgtggaact ttgaagcctg gaactggaaa cttctcctct
     3361 ttcgagtaga gaatcgctgt cttcggtcgc gacgtgcgta ggtaattgat gccgccgcgc
     3421 cgcgccattg tttccgtcag gcgctcggcg gacatcgcat cgctcggata caacaccgcc
     3481 gagccgtgaa cggctcggaa catggcaata tcctccagcg ccatctgtga agggccgtcc
     3541 tcgccgatcg agaccccgca atgagatccg cagaggttga tattcgcccg gctgatggcc
     3601 gccatgcgaa cattgtcata cgctcgcgaa aggaagcacg caaacgtatc gaggaatgga
     3661 accttaccgc gcgcggccaa cccgacaccg agactcacca gattctgctc cgcgatgtag
     3721 ccttggaaaa agtgatcggg aaaggccttc tggaatatct ccgaaaaggt ggaattcttc
     3781 acgtcccctg aagccgccac aatatgcgga ttcacctcgc caagccgctt cagcgcaaag
     3841 ccgtaggcct cacgtgttgc caccggcttg ccagcctcgt aatcgggcgc agcgggcgcc
     3901 ggaaaatccg gcggtttggg caaggaagag cgcgcatacg atttgcccgg atcgggcgga
     3961 acttcaatcg cccctcccag ttctttttca gctcgcgcga gttgctcttt tgaaaatggc
     4021 ttgccgtgcc aatgctcttt ccccgccacc tcgctgaaac cgtggccctt aatcgtccgc
     4081 gcgagaattg cttgcggccg gcccttcgtg cccttcgcgc ggtcaagcgc cgcaaggacc
     4141 gcaggtacgt cgtgcccgtc aatcacttca gcggcgaagc cttccgccgc gaatttctgg
     4201 cggtaaattt ccatatcatg ctggtacatc gtcgggtcgc tttgcccaag agcgttcacg
     4261 tccgcaatca ccgtcagatt gtccagcttg taatggcccg caaactccgc cgcttcccag
     4321 atttggcctt cggccatctc gccatcgcca aggaggacgt acacccgagt cgggctttta
     4381 tccaggcgcg caccaatggc aagacccgcg ccaaccgaga gcccctgtcc cagcgagccg
     4441 gtggcggcat ccacacccgg aattcgcggt gtagggtgcc cttcgagttc gctggaaaat
     4501 tgccgcagcg tcatcacccg cgaatcggga aacacgcccg cttcggcgag cgcggcatac
     4561 agcaccggcg cggcgtggcc cttcgaaagc acgaagcggt ctgcatccgg cgagttcgga
     4621 ttcttgggat cgaatttcat ggcgtagaaa aaaatcccag ccatcaattc ggctgcggac
     4681 atgcaggacg tgggatgacc cgatccggcg gcggtcgtct ccatcatgga aagaaggcta
     4741 agccttcgcg ccttttcttt gatcgtctct actgcggaag cggagggtgc tgcttgcgtg
     4801 gtcgcgctca tgggaacggc tccttagttc gtgcggctag gccgaaattg aagtttacaa
     4861 ctttgagccg aggtccgcga ggcccgattg gccccgcggc attatcaatg attcatgtgg
     4921 ttgccgcaga ttccaccgta attctttaca ccaaccgtgg acgacttttc tttacaccaa
     4981 caaccacttg cgctccccag gtgccttcgg ctaaaaatat taatgtatcc tcgtagtgtg
     5041 cccagacgca aagaaccgat ccatcctcca acctttacca tcgggcattc gacgcggcca
     5101 atcgacgatt tcattgcgct attgcgcgcg aatggcatcc gccaactgat cgatatacgc
     5161 acaattccca aatcgcggca caatccccaa ttcaatggcg acgcgctcgc aaaatcgctc
     5221 cgcgccgcgc gcattcaata tctccacatg aaggacctag gcggcctccg ccacgccaag
     5281 cccgattcaa tcaatctagg ctggcgcaat gcgagctttc gcggctacgc ggattacatg
     5341 caaactcccg agtttgaggc ggcgcttgag cgcgcgatca atctcgcaca aaagaaacca
     5401 accgcgctca tgtgcgccga ggcagtcccg tggcgctgcc atcgctccct cgttgccgat
     5461 gcgttgctcg ctcgcggcat ccctgtcctc gaaatcgtca gcagagccgg accaaaagag
     5521 cacaaactga cgccattcgc ccgcgtccgc ggcacaaaag tcacctatcc cagcgatcag
     5581 caatcgttgc tgccaatcta agtgctgttg gggaagaatg ccgctcagtg cggcggagga
     5641 actgctccgg gagcagcttt tgccggacgc tgcatgatga aagccacagg gaccaagcat
     5701 aggactataa ccgccagaat ggcgaccaca tcgcggaatg ccagcgccga cgcctgctgc
     5761 tgcatcatca tggcaatgcg aacatacgcc tggtgggtgg attcggccgc gctgatccct
     5821 ccgctccgca ggctggcggc gattccgtca accatggcgc gaaatcgcgg gttgccgttt
     5881 tgcatgttgg ccgagagata cacctgccgc gtctgcgtac cgcgcgtcac catcgtgctg
     5941 accaccgcga tgccgatgct cccgccgata ttgcggacaa aattcatcat gctcgaaacc
     6001 tgattatttt tttctcgcgg aatccccgcg tagcacagca cgttcgacgg gatgaagata
     6061 aatgccagcc ctgcaacctg gtacgtccgc agcatcgccg ccgtgccgaa atccatcgcc
     6121 agactcaaat tcgtcgccat atggtagagc cctaccgacg tggcggcgta
//
LOCUS       DATNDT010000136         4696 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_31549, whole genome shotgun sequence.
ACCESSION   DATNDT010000136 DATNDT010000000
VERSION     DATNDT010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4696
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31549"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="VN788_04300"
     CDS             complement(<1..593)
                     /locus_tag="VN788_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXU19786.1"
                     /translation="MPVYTYRGTNRMGAAVMGERQSQNKAQLAAALRRENINVSKLSE
                     KGKEFNIPTFGAGVDSKELAIFTRQFSVMIDAGLPLVQCLEILAGQQENKTFQKILNT
                     VRASVEGGATLSTSMKSHEKVFDPLYYNMVEAGETGGILDTILQRLSTYIEKNVKLKR
                     AVKSAMIYPISVLSIAAGVIILLLWKVVPIFITLFNG"
     gene            complement(625..1737)
                     /locus_tag="VN788_04305"
     CDS             complement(625..1737)
                     /locus_tag="VN788_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HXU19787.1"
                     /translation="MAGITLSELLKKMIEMSGSDLHLSTNSAPRVRVHGKLRPLDMPP
                     LTAADTKALAYSVLTDVQKHRLEENLELDFSFGLKSLARFRGNIFHQRGAVAAVFRTI
                     PWEIKSFDALGLPPIVRSLCDKPRGLVLVTGPTGSGKSTTLAAMLDKINNERDEHMIT
                     IEDPIEFLHNHKKCLVNQREVHSDTHSFANSLRAALREDPDIVLIGEMRDLETIESAL
                     RIAETGHLTFATLHTNSAVSTINRIVDVFPAHQQPQIRAQLSMVLEGILCQSLLPKID
                     GRGRAMVMEILIPNAAIRNLIREDKIHQIYSAMQTGTGATGMQTFNQSLANAHSQKLI
                     TLETALARSSNPDELQDLINRGVTSPPAGGRIPVRK"
     gene            complement(1750..3468)
                     /gene="pilB"
                     /locus_tag="VN788_04310"
     CDS             complement(1750..3468)
                     /gene="pilB"
                     /locus_tag="VN788_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HXU19788.1"
                     /translation="MSSRLGEILVKDSLISADQLKQALDYQKKNGGRLGTCLVKMGLV
                     SDDDITAVLSRQYGVPSINLKFYEVDPAVIKLVPQETAVRYQIVPLSRVGSTLTIAMT
                     DPTNVFAMDDIKFMTGFNVEPVVASETAISDAIHKFYGEVESVEELDKVMKDLTGEDE
                     DALELTGEEAEIDLATLSKAAEEAPIIKLCNLILTDAVKRGASDIHVEPYEKEYRVRF
                     RIDGILQNVMAPPMKLKDAMTSRIKIMSKLDISEKRLPQDGRIMIKYLKDGKKKELDF
                     RVSTVPTLFGEKIVLRLLDKENLRLDMTKLGFEPEALAKFERQILKPYGMVLVTGPTG
                     SGKTNTLYSSVARLNTSETNIMTAEDPVEFQLPGINQVQMKEQIGLNFASALRAFLRQ
                     DPNIILVGEIRDFETAEIAVKAALTGHLVLSTLHTNDAPSTISRLMNMGIEPFLVATS
                     VNLICAQRLVRRICVQCKEPLQIQAEALTDAGFSSEEAAKITVQHGKGCATCNNTGYK
                     GRVGLYEVMEINDELRELVLVGASALELKKKAMEQGMITLRRSGLQKVASGLTTMEEV
                     LRETVL"
     gene            complement(3848..>4696)
                     /locus_tag="VN788_04315"
     CDS             complement(3848..>4696)
                     /locus_tag="VN788_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HXU19789.1"
                     /translation="AEIYLHVFLASQDTNKKLIESSMKLVDQGCRDLLIAMFIRDGAV
                     RNLYGQPKFDDGTLPFDRDKETRKVLSACEIFRKEMPPEIGLGIDVHSLLDSVRATEF
                     CKDVEPFKLFYCEDLFPPEESGHYKIVRQMCTTPLAIGELWNNPHEWQPLVEQRQIDY
                     VRHHVSHIGGFTAARKVAAFAANYEVKFAWHGAPNSPVGHMTNLTLDLTNPNFGIHEH
                     FDYTPLVRDVFQGCLEVKDGYAWISEKPGWGIEVDEALAAKHPITDERPNEMRTRDGS
                     VINGTG"
BASE COUNT          968 a         1361 c         1216 g         1151 t
ORIGIN      
        1 aatccattga ataacgtgat gaaaatcgga acgactttcc agagcaacaa aatgatgacg
       61 cccgcggcga tgctcaaaac cgaaatcgga tagatcatcg cggacttcac cgcccgcttc
      121 agcttcacat tcttttcaat gtacgtggac aaacgctgca gaatcgtgtc caggataccg
      181 ccggtctcgc cggcctccac catgttgtag tagaggggat cgaaaacttt ttcgtgtgat
      241 ttcatcgagg tcgaaagcgt cgcgccgcct tcgacggaag ctcgcacagt gttcaggatc
      301 ttttggaatg tcttgttttc ctgttggccc gccagaatct ccaggcactg gaccagcggc
      361 aatccggcgt cgatcatcac ggaaaactgg cgcgtaaata tcgcgagctc cttggaatcg
      421 acgcccgctc cgaaagtggg gatgttgaac tccttgccct tttcggaaag cttgctgaca
      481 ttgatatttt cacggcgcag cgccgcggcg agctgagcct tgttctgcga ctggcgttcc
      541 cccatgaccg ccgcgcccat ccgattggtc cctcgatacg tataaaccgg catgatgtgt
      601 tacctccttc gtcgatctct tcggttactt tcggacggga atcctgccac cagcgggcgg
      661 cgacgtcacc ccacggttaa ttaaatcctg taattcgtct ggattcgacg atcttgcgag
      721 cgcggtttcc aaagtaatca acttctggga atgagcgttc gccaggctct ggttgaaggt
      781 ctgcattccg gttgctcctg ttcccgtctg catcgccgaa taaatctggt gaatcttgtc
      841 ttcgcggatc aggttccgga tggctgcatt gggaatcaat atttccatca ccattgctcg
      901 gccgcgtccg tcgatcttcg gaagcagcga ttggcaaagt attccttcta aaaccatcga
      961 aagctgtgcg cgaatttgcg gttgttgatg agccggaaaa acatcgacga tacggttgat
     1021 cgtcgagacg gccgaattcg tgtgcagggt ggcaaaggtc aggtgtcctg tttcggcgat
     1081 gcgcagtgcg gattcgattg tctctaaatc gcgcatttcg ccgatcagaa cgatgtccgg
     1141 atcttcgcgc agcgccgcgc gaagagaatt cgcgaacgaa tgtgtgtccg aatgtacctc
     1201 gcgctgattt accaggcact ttttgtggtt gtgaagaaac tcgatcggat cctcgatcgt
     1261 aatcatgtgc tcgtcgcgct cgttgttgat cttgtcgagc atcgctgcga gcgtcgttga
     1321 ttttcctgag cctgttgggc ccgttaccag caccaacccg cgcggtttgt cgcaaaggct
     1381 gcgtacgatc ggcggcagtc cgagcgcgtc gaaacttttg atttcccagg gtatcgtcct
     1441 gaaaacggcg gccaccgcgc cccgctgatg aaaaatgttg ccgcggaacc gggccaggct
     1501 cttcaatcca aacgaaaagt ccagttccag gttttcttcc agccggtgtt tctgcacgtc
     1561 tgtcagcacg ctgtacgcca gcgccttcgt atcggccgcc gtgagaggcg gcatgtccaa
     1621 gggacgaagc ttcccatgta cgcgcactcg cggggcgcta ttggtcgaga gatgcaaatc
     1681 gctccccgac atctcgatca ttttcttcaa gagttcactc aatgtgatgc cggccatttt
     1741 ctcctccgat tacagaaccg tctcgcgcaa aacttcttcc atcgtcgtca gtcccgatgc
     1801 aactttctgc aaaccgcttc ggcgcaacgt gatcattccc tgttccatcg cctttttctt
     1861 cagttccagc gccgaagctc ccaccagcac cagttcgcgc aattcgtcgt taatttccat
     1921 cacctcataa agtcccacgc ggcctttgta tcccgtgttg ttgcaagttg cgcatccttt
     1981 gccatgttgc acggtgattt tcgccgcctc ttcggaagaa aaccccgcat ccgtcagcgc
     2041 ctccgcttgt atctgcagcg gttccttgca ttgaacgcaa atgcggcgca ccaaccgctg
     2101 cgcgcagatc agattcacgg atgtggccac caggaacggt tcaatgccca tgttcattaa
     2161 ccggctgatc gtggagggtg catcgttcgt atggagcgtg gaaaggacca agtggccggt
     2221 gagcgctgcc ttcaccgcga tttcagccgt ctcgaaatct cgtatttctc ctaccagaat
     2281 aatgttggga tcctgccgaa ggaatgcgcg cagcgccgac gcaaagttca acccgatctg
     2341 ctccttcatt tgcacctggt tgatccctgg aagctgaaat tcgaccgggt cctcggccgt
     2401 cataatgttc gtttcggacg tgttcaagcg cgcaaccgat gaataaagcg tatttgtctt
     2461 gcccgatccc gtgggtccag tcaccaggac catgccgtaa ggcttcagga tttgccgctc
     2521 gaatttcgcc agcgcctccg gctcaaagcc gagcttggtc atatccagcc gcaggttttc
     2581 tttgtccaac aaccgcagaa caattttttc cccgaaaagc gtaggcactg tcgaaacgcg
     2641 aaagtcgagc tccttcttct ttccatcctt caggtacttg atcatgatgc gtccgtcttg
     2701 cggcaggcgc ttttcgctga tgtccagttt ggacatgatc ttgatgcggc tcgtcatcgc
     2761 atcttttaat ttcatgggcg gagccatcac gttctgcagg atcccgtcga tgcgaaaccg
     2821 gacgcggtat tccttttcgt agggctccac gtgaatatcg ctcgcgccgc gcttcacagc
     2881 gtcggtcagg atcagattgc agagcttgat gattggagct tcttcggcgg cctttgaaag
     2941 cgtcgccagg tctatctccg cttcttcacc ggtgagctcg agagcatcct cgtcctcgcc
     3001 cgtcaggtcc ttcatcacct tgtcgagttc ttcgacgctc tccacttccc cgtagaactt
     3061 gtgaatggca tcgctgatcg ctgtttcaga cgcaactacc ggctccacgt taaaacccgt
     3121 catgaacttg atgtcgtcca tggcgaacac attcgtcgga tccgtcatcg cgatcgtcaa
     3181 cgtcgatccc acgcgtgaga gcggcacaat ctggtagcgc accgccgtct cttgcgggac
     3241 cagcttgatc accgctgggt ctacttcgta aaatttcaaa ttgatggaag gcacaccgta
     3301 ctgccgggag agaaccgcag tgatgtcgtc gtcgcttacc agccccatct tcaccaggca
     3361 ggtgcctagg cggccgccgt ttttcttttg atagtcgagc gcctgcttca gctgatctgc
     3421 ggagatcagg ctgtccttga ctagaatttc acccagccgg gaagacataa ttgtgcgccc
     3481 gcagtactag aactgcggta ttcgggagag tagcatgtga ttcgcagcga cggccgccct
     3541 cgcttcaccc ctcgtagtga atcagctaac atccagtccg ccgcatcaca tttctgtact
     3601 ttcgtacagg ccatttactg acctgtactt aggtacaggt gctttccagt accttatcgg
     3661 gcgctgggcc agtccactcc gccgcctgtt ggtttgaagg tttcgaaggc agaactaagc
     3721 cgtgcgaacg gccagcgtga tggcgaaaag cctgctgggt ttgagtaaag gggataaact
     3781 tcgcggaatc gatgccgcca tccgacgcgc gatacgctgt ggatggctaa accaataaag
     3841 tctgattcta gccggtgccg ttgattacgg agccatcccg cgtgcgcatc tcattaggac
     3901 gttcgtcggt gatggggtgc tttgccgcca gcgcttcgtc aacctcaatg ccccagcccg
     3961 gcttttcgct gatccacgcg tagccatcct taacctcaag gcagccctga aaaacatcgc
     4021 ggaccagcgg cgtgtaatcg aaatgctcat gaatgccaaa attcggattg gtcagatcga
     4081 gtgtcagatt ggtcatatgg cccacaggag agttgggagc gccatgccag gcgaatttca
     4141 cctcataatt tgccgcaaac gccgccacct tccgcgcggc cgtgaagccg ccgatatgcg
     4201 aaacatggtg acgcacgtag tcaatctggc gttgttcgac gagcggctgc cactcatgcg
     4261 ggttattcca tagctcaccg atcgccagcg gcgtcgtgca catctgccgg acgatcttat
     4321 agtgaccgga ctcttccggc ggaaacagat cttcgcaata aaacaatttg aacggctcca
     4381 catccttgca aaactcagtg gctcgcacgc tatcgagcag cgaatgcacg tcgatcccaa
     4441 ggccgatttc cggcggcatc tcttttctga aaatctcgca cgccgatagg accttgcgag
     4501 tttccttgtc acgatcgaaa ggcagggtgc cgtcgtcgaa tttcggctgt ccatacaaat
     4561 tgcgcacggc cccgtctcga atgaacatcg cgatcagcaa atcccggcag ccttgatcga
     4621 caagtttcat tgagctttcg atcagcttct tattggtatc ctgcgaggcc aggaacacgt
     4681 gcaggtaaat ctccgc
//
LOCUS       DATNDT010000137         3627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4088079, whole genome shotgun sequence.
ACCESSION   DATNDT010000137 DATNDT010000000
VERSION     DATNDT010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4088079"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..800
                     /locus_tag="VN788_04320"
     CDS             204..800
                     /locus_tag="VN788_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXU19790.1"
                     /translation="MKLIPSLEEPVRTIVLVAVLTGLRIGELLALRWKHLDFLCGAIQ
                     IRETVSEGKFGTPKTKSSRRDVPMSDPVREALLAQRGRSRQTGSDDLIFATRKQTPLN
                     PKNLLRRQLRPACAALELPLVSWHSFRHTHATLLGESGESLKTAQALLGHSDLGTTLN
                     VYTHAIPESQKRAVDKVAEILFPNVPKFSGNLERKDVN"
     gene            complement(829..904)
                     /locus_tag="VN788_04325"
     tRNA            complement(829..904)
                     /locus_tag="VN788_04325"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(869..871),aa:Thr,seq:cgt)
     gene            1027..1500
                     /gene="tsaA"
                     /locus_tag="VN788_04330"
     CDS             1027..1500
                     /gene="tsaA"
                     /locus_tag="VN788_04330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HXU19791.1"
                     /translation="MFTPQAIGFVRSPYTGTKQIPKGLGTKHEAEGVLEIQPEFERGL
                     ADIEGFSHLFVIWVFDRSQGFELVGSPPFDNRPHGVFATRSPRRPNPIGLTVVELLRR
                     DGLMLHVRGLDMLDGTPILDIKPYMSSVPPEKLRRGWLAEAETRSSKECRRLPGD"
     gene            1574..2761
                     /locus_tag="VN788_04335"
     CDS             1574..2761
                     /locus_tag="VN788_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXU19792.1"
                     /translation="MSNFDIIVVGGGLAGSSFAIAMAKHGAKVLVLEQESRFRDRIRG
                     EFLCSWGTAEVKALGLLETFRNSSGRDIPWVDMGTGPRDLLTTTKSQLPGFSYSHPQL
                     QQGLIEAAQKAGAEVRRGIAVQKIEPGVIPVVECQGNGSPQRITARLVVAADGRNSAA
                     RRWGGFTSLRDEHPFLLAGVLLEGASFRPDLCAFVFNPDAGTITGTIPQTGGLTRAYF
                     AYPLAAGYRMQGKERLSLLLAESVKSSPFLAEAYANARDVGPLASFDANDSWVDHPYK
                     NGVALIGDAAATSDPCFGQGMSLLMRDVRVLRDSLIANSDWDRAGNLYAEAHDSYFHP
                     CHKACQMLRTVFQEQTPEAALIRQRALPLIAEDPARVPDHLLSGPDLPTDESVRARFF
                     GEC"
     gene            2805..3269
                     /locus_tag="VN788_04340"
     CDS             2805..3269
                     /locus_tag="VN788_04340"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HXU19793.1"
                     /translation="MKKYKFKARIEEGQGGGADVLFPYDTQKEFATNGKVPVKATFEG
                     VPYAGSLVKYGHPHHMLGMPKAIREQIAKRPGDTVQIVVWKDEEVRTVQVPAEFERLI
                     KKEGLLSVFNELGYTHRKEYCRWITDAKKEETRLRRLQKAIQMLKKGVRTPG"
BASE COUNT          811 a         1080 c          987 g          749 t
ORIGIN      
        1 gggtagacgt tcaatgcttt gttgccgaaa agcgaaggcg cggctattcg ggttcgagta
       61 ttcacggcat gcggactgca cttgggtagg cactacaatc cgcggtcgag tggggctacg
      121 tcgagcagaa cgcagcgcgc ggcgtcaata tcggtagccg cgagccgaaa acggaaaggc
      181 tctacctctc agccgcggaa gcagtgaaac tcattccgtc actcgaggag ccggtccgca
      241 caatcgttct cgtggccgtg ttgacgggcc ttcggatcgg tgaactgctc gcccttcgtt
      301 ggaagcatct cgatttcctt tgtggggcga ttcagattcg cgagactgtt tccgaaggca
      361 agttcggcac accgaaaacc aaaagcagcc ggcgggacgt tccgatgagc gacccagttc
      421 gtgaggcttt actcgctcag cgcgggcgtt cgcggcaaac tgggtccgac gatctcattt
      481 tcgccacccg gaaacaaaca ccactgaacc caaagaatct tctgcgtcgc cagcttcgac
      541 ccgcatgcgc agcgctcgaa ctgccgctcg tttcctggca cagtttccgg cacacgcacg
      601 cgacgctgct cggtgaaagc ggagaatcac tcaagaccgc ccaggcgcta ctggggcatt
      661 ccgatcttgg gacaaccctc aacgtctaca cgcacgcgat tcccgaatcc cagaagcgtg
      721 cagtggacaa agttgccgaa attttgttcc caaatgttcc caaattttct ggtaacttgg
      781 aacgcaagga tgtcaactga ctgatgccgc aagaacttac aaagcggatg gagccgaggc
      841 ccggacttga accggggacc tgccgattac gaatcggctg ctctaccaac tgagctacct
      901 cggcggtgta atcgactgta gcatccgccc gaaaaacctg tcaaacaaac aggcccgcgc
      961 gtagtgtcct aaaatattac tttcatgtgc tcgggtgcga aatttgccaa ggcaggaggc
     1021 cgctaaatgt tcacgccaca ggcgattgga ttcgtgagaa gcccctacac gggcacgaag
     1081 cagattccga aaggtctcgg cacgaaacat gaggccgagg gcgtgctcga aattcaacca
     1141 gaatttgagc gcggcctcgc tgacattgaa ggcttctcgc accttttcgt catctgggtg
     1201 ttcgaccgct cccaaggatt cgaattggta ggctcgccgc ctttcgataa tcgtccgcac
     1261 ggagtcttcg ccacgcgctc gccgcgacgt cccaatccga tcggccttac cgtcgtcgaa
     1321 ctccttcgcc gagacggcct aatgctgcac gtccgcggcc tcgacatgct cgacggcacg
     1381 cccatcctcg atatcaagcc ttacatgtca agcgtcccgc cggaaaaatt aagaaggggc
     1441 tggctcgcgg aagcggaaac ccgcagcagc aaagaatgtc ggcgcctgcc tggagattga
     1501 ctcgcgggcc gcgtcgcgtt taccatcctg aacaataaaa atccctgagc cagaccggac
     1561 ggatcatttc gccatgtcga atttcgatat catcgtggtt ggcggcggcc ttgcgggctc
     1621 ttcgtttgcc atcgcaatgg caaaacatgg agccaaggtt ttggttcttg aacaggagtc
     1681 ccgcttccgc gatcgaattc gcggcgaatt tctttgttca tggggtaccg cggaggtaaa
     1741 agcgctcggt cttctcgaaa ccttccgcaa ctcgagcggc cgcgacattc cttgggtcga
     1801 tatgggcacg ggcccgcgcg acctgctcac gaccacgaag tcgcagcttc ctggtttttc
     1861 ctactctcat ccccaactgc aacaaggctt gattgaagcc gcgcaaaaag ctggtgccga
     1921 ggttcgcaga ggtatcgccg ttcaaaaaat tgaaccgggc gtgatccctg tagtcgaatg
     1981 ccagggaaac ggctcgcccc agcgaatcac cgcccgcctg gttgttgctg ccgacggccg
     2041 caactcagcc gccaggagat ggggcggatt cacttcgctg cgagacgagc acccattcct
     2101 cctcgccggc gtgcttttgg aaggagcatc attccggccc gacctttgtg catttgtctt
     2161 caatcccgat gctggcacta tcaccggaac catcccgcag accggtggct taacgcgcgc
     2221 gtatttcgcc tatccgcttg ccgccggata ccgcatgcag ggaaaggaaa ggctctctct
     2281 cttgcttgcg gaatccgtga agtcctcgcc gtttctcgct gaggcatacg ccaacgctcg
     2341 cgatgtcggg ccgctcgcct cattcgatgc caacgattcg tgggtagacc atccgtataa
     2401 gaacggcgtc gcgttgatcg gagacgccgc tgccaccagc gacccatgct tcggccaggg
     2461 aatgtctctc ctgatgcgcg atgtccgcgt cctacgcgac tcgctcatcg caaattcaga
     2521 ctgggaccgc gccggcaatc tttacgcaga agcgcacgac tcctattttc atccctgcca
     2581 caaagcgtgc caaatgctgc gcaccgtctt ccaggagcag acgccggagg ccgcgctcat
     2641 ccgccagaga gcgctacccc tcatcgcgga ggaccccgca cgcgtacccg accatctgct
     2701 gagcggaccc gacttaccca cggatgagtc ggtccgagcc cgcttcttcg gcgaatgcta
     2761 aaaccgcgat ttcgaaaagt tcgcgcgcga atttttgcaa atctatgaag aaatacaaat
     2821 tcaaggcgag aattgaagaa ggccaaggcg gaggcgccga tgtccttttt ccgtacgata
     2881 ctcaaaagga atttgccacc aatggaaagg tgcctgtaaa ggcgaccttc gagggtgtgc
     2941 cttacgctgg ttctctcgtc aagtatggtc atccacatca tatgctgggt atgccgaagg
     3001 ccatacgcga gcaaatcgcg aagcggccgg gcgatacggt tcagatcgtt gtctggaaag
     3061 atgaagaggt aagaaccgtc caggtgcccg ctgaatttga aaggctaatc aagaaagaag
     3121 ggctgctttc ggttttcaac gaactgggct acacccaccg caaggaatac tgccggtgga
     3181 ttaccgatgc caagaaggaa gaaacccgat tacggagatt acagaaagct attcaaatgc
     3241 tcaaaaaggg agtgagaact ccagggtagc cctgactgtc tgtgtcgtcc cccgccgcaa
     3301 tttagcgctt ggttcctgcg ctcagctggc aagccgcgct tacaattctt tacaccaaca
     3361 accacttgcg tcgtgtcccg ccgggtgtca cgctaatctt ggaagtatgt gcggtccctg
     3421 ggagcgccat tctcctgaat ggcgcctttc ggcccgccac aaaaaattga gaaaggaggc
     3481 agcagcagga tgcgacaaaa cgccgcaggg gtttcccagc catcgcggga tattcagact
     3541 ccgcatccgt tcgcgcaagc ttcaccccgc gtcttcttca acatcttatc gacacttctg
     3601 ccgagtagaa tcattcgtaa ttcattg
//
LOCUS       DATNDT010000138        15570 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3693342, whole genome shotgun sequence.
ACCESSION   DATNDT010000138 DATNDT010000000
VERSION     DATNDT010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 15570)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15570)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15570
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3693342"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..115
                     /locus_tag="VN788_04345"
     tRNA            29..115
                     /locus_tag="VN788_04345"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:63..65,aa:Leu,seq:gag)
     gene            406..1173
                     /locus_tag="VN788_04350"
     CDS             406..1173
                     /locus_tag="VN788_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HXU19794.1"
                     /translation="MATLVAMTVYIFASRKYSAPSPITSVGLQIDRQYDLTLYVTGAA
                     FILAQLVLGYAILRFRDRGQQAHFTHGSTVAEVLWTSVIIISFLGLGIAGSKTWGEMR
                     FTPASRDSVQVEVTTTQFVYTFRYPGADGKFGRLDASLVNAPAGNPLGLDPNDATGRD
                     DVVVPILTVPVNHPVELLLRSQDVIHNFFVRELRLQQDAVPGMMIPIHFTADRIGRYE
                     IVCTQLCGLGHYKMHSFMDVVSERDYERFLKKQGPPQ"
     gene            1229..3007
                     /locus_tag="VN788_04355"
     CDS             1229..3007
                     /locus_tag="VN788_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HXU19795.1"
                     /translation="MSSPARDVHATSGAPGSFVRRYIFSSDHKVVGLQYFFLALTAVT
                     IGIVLSLLMRFHLVWPAAHLPFVAGGIMEPEQYLALVTMHGTLMVFFVLTVAPQSAFG
                     NYFLPIQIGAPDMAFSKLNMLSFWLTFIAFLCLIAAFFVRGGAPISGWTAYPPLSGLG
                     EVTGPGEGLGQTLWIVSILIFCFGSLLGSVNFIATIIDLRAPGMTLMRMPLTVWAWLV
                     TAVLTLLSFSVLLAGGILLLLDRSAGTSFFLPAGLIVNGEVQSHSGGSPLLWQHLFWF
                     FGHPEVYIAILPGMGAVSHILANFSRKPVFGYRAMAATLCAIGFLGFLLWGHHMFVSG
                     MNPYSGIAFSLLTLSIGVPSAVKTFNWLGTLWGGKIQFHSPMLFAIGFVSFFVTGGLS
                     GIFLAQPSLDSVLHSTYYVVGHFHLVMGVASIFGIFAATYYWFPKMFGKMMNETLGKV
                     HFWLTFAGAYCIFMPMHFVGTAGGIRRYADWTGANYLAALQPLHRFMTIAAFATAAAQ
                     IIFFYNFFRSLAKGARASANPWSGTTLEWTTASPPPFDNFNGHYPRVYRGPYEYSVPG
                     ASQDFLPQNIAPGKSLTDDVESQHAG"
     gene            2997..3656
                     /locus_tag="VN788_04360"
     CDS             2997..3656
                     /locus_tag="VN788_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXU19796.1"
                     /translation="MPDSALTEFVKSDNANGMGNGGASRTDGPNAGGPSPFDGGRVPQ
                     RIYMTGMMIALGAIFMFFVALASASIVRKGLGSTDWQPFELPRILWLNTLLLVASSLT
                     LANARRRFLAHQFAEFRHWWGVTTILGIFFLVGQIIAWWKLASSGVFLSTSPNSGFFY
                     VFTVAHGLHLLGGTAGLLAVFWWPPGPMLRKTATEVVALYWHCMTALWLGVFVFLVAT
                     K"
     gene            complement(4008..5087)
                     /locus_tag="VN788_04365"
     CDS             complement(4008..5087)
                     /locus_tag="VN788_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXU19797.1"
                     /translation="MAHEKILVVDDEEAIREVISTLLDAQGFQCTTCVNGKVGLETFR
                     KDSYDLVLSDIVMPEMDGLKLLAELRMADPDVPAIMVTAMHDISIALEAIRAGAYDYI
                     LKPFEKDQLYLSVRRALEHRRLVMENRTYQSDLEQLVAERTRQLSIALQDLEQSYDYT
                     LEALGGALDAKDAETEGHCQRVTAFTITIAKAMGVDSSLLRQIARGAFLHDIGKMGVP
                     DQILRKPGPLTAQEREIMRRHCDIGYAVLERIPFLKEAAEIVLAHQEFYDGTGYPRGL
                     RGEEIPLGARIFAVADTLDAMISDRPYRKALPISAAREEIERFSGTQFDPQVVKVFLA
                     QPEQLWRELHTNSGDPFLLTQLEAV"
     gene            5259..8390
                     /locus_tag="VN788_04370"
     CDS             5259..8390
                     /locus_tag="VN788_04370"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU19798.1"
                     /translation="MTFASNSGFLAIAFMEGSAAFILLIVYWLLLPSFPARFFRYWLV
                     GWTLYTALQGLRIFSIWRGGPDMPHSGQALSFLAAVFFLASVLDCRDPGKPVWKLWPL
                     WLFAASDVAALAWVGRIHYLPNWSETLLECSLYLSAGWIFWGSQARHRGVGWKLLAGA
                     LLLRGLHGLDQPQWAAHSAGLFRVSFQGLFGIMMGIAMAVLVLEAGRGRTEDLNEKLR
                     RLSLITAEATQSLRVDEVLQGVLSHLVESLGGSHGLVFLVDDSKQPPALACRGSVGFS
                     ERFRKQRPQISADEQWLQQLLLREKPLTLSLQTKNVAPRSWMETEKLASLMLVRIPGK
                     DEPRGFLAIGSLTPRDFERDEQDFLVNVANLLGLTAQNVALFQNAATARRQWLDTFDS
                     MDDLILVHSLDGIILRANRALADRLGAELSTLVGRSVRDVLHSSGFGWSRCPYCEGVA
                     GKADEIDPSFGGYFLASDSSLHDSEGGNLGTIHVLKDLTDRRQAENKFRTLFEKVQEG
                     IFISTPDGRFLDFNDAFMRILGYESHDELLQADIPAQLYVNPADRDRRQRLLNEYGEV
                     TDFEFQFRRHDGDIRTAHESSFATRDDSGTVIAYQGFLLDVTDQKQAEFDVRRRNRAL
                     LALNNIGELLGQSQSLEEGLTASLAKVAELFATDFGTVYLLDETSNSLKRAAAIGCRS
                     NQAARPEPIAIPAALLEQIRQAHATLLSGSLPSLPEALREFHEREGIQSSQLAMLWAK
                     DSVVGALVIGSREARAFSTAELNLLSAVGNQIATTIDKSLLLEKTREAYDSLRRTQEQ
                     LLQSEKMAAVGQLISGVAHELNNPLTAILGYTQLLKSDELVLPRGLDYVEKLYKQAQR
                     THHIVQNLLSFARQQKPERASVQINQILEETLILREFDMKYNNIVIRREFDAQLPPTG
                     GDFHQLEQVFLNILNNAVDAIQETGAPGEITIRTEVAGNRLCVEFRDNGPGVTNPHRI
                     FDPFYTTKPVGKGTGLGLSICYGIVKEHGGEIRVRNVEPRGAAFTVTLPLLVVGPSEP
                     AELASPVSTVSGS"
     gene            complement(8428..10584)
                     /locus_tag="VN788_04375"
     CDS             complement(8428..10584)
                     /locus_tag="VN788_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU19799.1"
                     /translation="MNSAVRAATAFLVLCFGFHSAGAIHLATAQNTSTTAGVAKRIQA
                     GKKLYASGCVFCHGVDGNGSGAAVSLRKLDLSAEKITTIISEGKPGTAMPAFKGNYSS
                     EQIGDLADYVLSLKKNGESAAEARPATASNSPEAEGAAIYASKCASCHEVGTAPLFNH
                     YILKSMSPDYILYVLRSGAMRELAANMPFRQRVAVAEYLTGKRAGSSRPTNPLAGQCT
                     GRASSDFSGPEWNGWGVDMDNSRFQPADQAGLSAAQVPKLKLKWAFGFPADWGAYAQP
                     VIAGGRVFVGSSMGMVYALDEKTGCTYWKFRAEGAVRSAVVLGPGNLAYFGDYRTNIY
                     AVNAITGKLVWKTRISDHPYARVTDAPKLYEGRLYAGVSSREEWMASDPHYECCTFRG
                     VLVSLDAATGKQIWKTYTISTPPQPTTTKPDGTRRWGPSGAAIWSSPTVDAKKGLLYV
                     GVGDNYSDPPTPLSDSILAVDMKAGKIVWSKQLTAGDAFNTSCIQKNMVNCPAKPGGD
                     YDFAAPPLLRPLPSGQRVLILSQKSATVLAIDPDKQGAILWKAQIGKGGPLGGIEWGP
                     AADAKVVYEALSDLDVISFPEGIKGDSTKGGGLFALDIATGKRIWTAPPVLGTCHVAR
                     CTPAQSAAATAMPGVVFSGSDDGHMRAYSAENGKIEWDFDTAHDFKTVNKVPAHGGSI
                     DGGGGAAIAGGMVFVNSGYGALFGMPGNVLLAFSPE"
     gene            complement(10705..11778)
                     /locus_tag="VN788_04380"
     CDS             complement(10705..11778)
                     /locus_tag="VN788_04380"
                     /inference="COORDINATES: protein motif:HMM:NF019468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5808 domain-containing protein"
                     /protein_id="HXU19800.1"
                     /translation="MIQHSEWIFGPFLLALAALVTWAFWMLPQWTRPGIYFGVTVAPD
                     FGNTPEAERLLQRYRLEAMIHVAIAFGMVLVGALPGYSFLPIIGGLWLAVGPLTAFVE
                     AHKRVKRYAVRAVTVREATLEPRNTRPPGGWFVQVGPFAILLAVGLFLHFHWDEIPAR
                     FPVHWGIDGRPNGWSSRTQMGVYAPLLFGALISACMWLTANTLLRSARITDRARPVLV
                     LHDFAHRVGLFLLLIEFFLAVIFSCVGLLPLAGAMPVLIVTILMLPAVFVLIVWLNKG
                     RAHIERSGETNPTAAGDGTPDECWKFGMLYFNPDDAALWVEKRAGIGYTMNFAHVSAW
                     IIMALILLLPLMLGLVAVHNQLK"
     gene            complement(11775..12179)
                     /locus_tag="VN788_04385"
     CDS             complement(11775..12179)
                     /locus_tag="VN788_04385"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXU19801.1"
                     /translation="MKSPRTLHIDLAAAQPVYEQIVSGLRALLVSGAFESGEQLPTVR
                     RLAIDLGVHHNTVAEAYRILAEEGWLDLRRRRGAVVLDRRDPKATPEAKQDFRKHLEE
                     LIAKAITNGVPRSVIAGRMADIAKKLGHGGTR"
     gene            12372..13784
                     /locus_tag="VN788_04390"
     CDS             12372..13784
                     /locus_tag="VN788_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19802.1"
                     /translation="MKRWIGLLFVLAVGITAAARQSASPSAALPLNSVSSQAPVAPNP
                     EFLKTADEVLQQMSVILHLPIDQPLKKTLRSKQEIRDYLIREEKEDKNEAQRYADTKS
                     LEAFGLIPKGFPLDSFMLDVLTDQVAGLYDPKAKEFYIADWIPASEQREVMSHELTHA
                     LEDQSFNVDPWIKAARPNDDAEMARDAVSEGTAMAAMVDYALRDQNVGVRDLVDVSML
                     IRAGAVAEMNNDPKLSKAPLYIRDGLLFPYLAGTSFAQQFLKAHTGWSDLHLLFEHPP
                     VSTQQILHPDFYLKDVGPAKVTLPAWKGLVPADWKLLEENVMGEFGLEEVLKQFLDPA
                     RAAALSPAWDGDRYAVFEDTKTKETPLVFHLALETPDDTARFFGQYSEALETKYKTRT
                     ALYRRPNFFQFQTDAGGVFLRCMELQCLSVERATRATFDTINRAIGWPAAPGPVEAEP
                     SVAQLREAKPAEATRAPRSH"
     gene            13804..14496
                     /locus_tag="VN788_04395"
     CDS             13804..14496
                     /locus_tag="VN788_04395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU19803.1"
                     /translation="MSIFELQRVAEPEVMDDSAEVEAYSSAAAQSHLNDIDDTFVDHA
                     TRLVNKRERGRALDIGTGPGQIVLKLARRLTRWKFIGVDRSPNMIAQGIAGLAPSTEV
                     AGRVHFYIADGNQIPFCDGSFDLVACNSVLHHFADPQKLLAEIARLAKPESAILLRDL
                     RRPSRLAYPLHVRRHGRHYQGLMYKLYCDSVRSAYTVEELQRLLGSSPLKGARIFEHN
                     HTHIGIERPISS"
     gene            complement(14514..>15570)
                     /locus_tag="VN788_04400"
     CDS             complement(14514..>15570)
                     /locus_tag="VN788_04400"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXU19804.1"
                     /translation="ARISEPENKAASGITDPYLREQLEKRRAELKTNIAVIPVDDPEA
                     ATPLLDLLQEVDSAMERMDEGTYGICEACHDTLGSERLISDPLAQMCLDCLTGEEQRA
                     LERDLELAARIQRGLLPQTDTRFQDWRIHYRYKPAGVVSGDYCDVIPSATGDGKVIFL
                     LGDVTGKGVAASLLMTHLHAMFRSLAGIGLELDKLLEMANRLFCESTIAGQYATLVCG
                     RIGRDGEVEIASAGHFPALLLGKNGVSRIEATGLPLGMFTTSSYAVRRARLEPDDSLL
                     LYTDGISEARDPSGNEYGISGLSIAASEKHGLTPEALVAACMKDLERHSSGTRQADDQ
                     TLMAIQRAQSGHSSFND"
BASE COUNT         3074 a         4720 c         4242 g         3534 t
ORIGIN      
        1 aatcctcgcc gcttaggatt gacgcggcgc ggtcgtggcg gaactggcag acgcaccagc
       61 ttgaggggct ggcgctcgca agagcgtggt ggttcgaatc cactcgaccg caccaacatt
      121 tccgacgagg aaatgttgcc cgagcgaacg gagcgagccg aagggaattc ccgtatcaga
      181 caaccactcg accgcaccaa acctaccgca aaaatcctgg tgccaagctc ggttacatcc
      241 gctgctcttt gaaatgtcct accggttttg tatgatggcg cttcgtaaaa gacgatgcga
      301 ccttcccgat tgttttttac cgatggattc acgcggataa attctccccg caccgccgcg
      361 cataatcggg gctccgctgc accttcagtt ctgctggcgg ttttattggc gacactggtt
      421 gcgatgaccg tctatatttt tgcctcacgc aaatactcgg caccttcgcc gatcacttct
      481 gtcggactgc aaatcgatcg gcagtatgac ctcacgctgt acgttaccgg agcggcattc
      541 atcctggcgc agcttgtact tggttacgca atacttcggt ttcgcgatcg cggccagcag
      601 gcccacttca cgcacggaag cacggttgcc gaagttttgt ggacttccgt catcatcata
      661 tcgtttctcg gcctcggaat tgctggaagc aagacctggg gagagatgcg tttcacgccg
      721 gcgagccgcg attccgtgca ggtagaagtt accaccacgc aatttgttta caccttccgc
      781 tatccgggcg ccgatggaaa attcgggcgt ctcgatgcga gcctggtgaa cgcgccggcc
      841 ggtaatcccc tcggcctcga tccgaacgat gcgacgggcc gcgacgacgt cgttgtcccg
      901 atcctgactg tcccggtgaa tcatccggtg gaactgctgc tgcgctcgca ggatgtgatc
      961 cacaattttt ttgtgcggga gttgcggctt cagcaagatg cggtgccggg gatgatgatt
     1021 cccattcatt tcacggcgga tcgaattggc aggtacgaga ttgtctgcac gcagctatgc
     1081 ggactgggtc attacaagat gcacagcttc atggacgtgg tgtcggagcg cgactacgag
     1141 cgattcctaa aaaagcaggg acccccgcaa tgatagtggt actgactctg ccgtgtccta
     1201 cgcgccggca ttccacgcga ggccgcgcgt gagttctccg gcccgcgatg tccatgccac
     1261 cagcggcgcg cccggcagtt ttgtgcgacg gtatatcttc agctcggacc acaaggttgt
     1321 gggcctgcag tatttctttc tcgccctcac ggcggtgacg atcggaatag ttctttcgct
     1381 cctgatgcgc tttcatttgg tgtggccggc ggcgcatctt ccctttgtag cgggcggcat
     1441 catggaaccg gagcagtatc tcgcgctcgt caccatgcac gggacgctga tggtgttctt
     1501 tgtgctcacg gttgctcccc agagcgcttt cggcaattat tttctaccaa tccaaatcgg
     1561 cgcgcccgat atggcgtttt cgaaactcaa catgctttcc ttttggctga ccttcatcgc
     1621 ttttctctgc ctgatcgccg ctttttttgt tcgcggcggc gcgcccatca gcggttggac
     1681 ggcctatccg ccgctgagcg gcttgggaga agttacgggt cctggcgagg gcctcggcca
     1741 aacgctgtgg atcgtcagca tattgatctt ttgctttggc tcgctgctgg gctcagtgaa
     1801 tttcatcgcc accatcattg acttgcgcgc cccaggaatg acgctgatgc gcatgcctct
     1861 caccgtttgg gcctggctgg tcactgcggt tctcaccctg ctctcgtttt ccgtgctgct
     1921 tgcgggtgga attctgctgt tgctcgaccg ttctgcggga accagctttt tcctccccgc
     1981 cggtctgata gtgaatggag aagtgcaaag ccatagcgga ggatcgccgc tcctctggca
     2041 gcatcttttc tggtttttcg ggcatccgga ggtctacatc gcgattttgc ccggcatggg
     2101 cgccgtctcg cacatccttg cgaacttttc gcgcaagccg gtcttcggat atcgggcaat
     2161 ggctgccact ctatgtgcga tcggttttct gggctttctg ctctggggcc atcatatgtt
     2221 tgtgagtggt atgaacccct actcaggaat tgcattctcg ctgctgacgc tctcgatcgg
     2281 agtcccgtcg gcggtaaaaa ccttcaattg gcttggaaca ctgtggggcg gcaagattca
     2341 attccattcc ccgatgcttt ttgccatagg ctttgtttca ttctttgtca ccggcgggct
     2401 ttcgggaatt ttcctcgcgc aaccttcact agatagcgtg ctgcactcca cctattacgt
     2461 agtcggtcat tttcatctcg taatgggcgt agcgtcgatc tttggaattt tcgccgcaac
     2521 gtattactgg tttccaaaga tgttcgggaa aatgatgaac gagactctgg gaaaggttca
     2581 tttttggctt acattcgccg gagcatactg catcttcatg cccatgcatt ttgtgggcac
     2641 cgctggcgga atccgccgct acgcggattg gacgggcgcg aattatctgg cggccctcca
     2701 gccgctgcat cgctttatga ccattgcggc attcgccacc gccgctgcgc agatcatctt
     2761 cttctataat ttctttcgga gccttgccaa aggcgcgcgc gcttcggcca atccctggag
     2821 cggcacgacg cttgaatgga caactgcgtc gccgcctccg ttcgacaatt tcaacggcca
     2881 ttatccgcga gtttatcgcg gcccttacga atacagcgtt cccggcgcaa gccaggattt
     2941 tcttccccag aacatcgcgc cgggaaaaag tttaactgat gacgtggagt ctcagcatgc
     3001 cggatagcgc cctcacggaa ttcgtcaaat cggacaatgc aaacggtatg ggaaacggtg
     3061 gcgcctcgcg aaccgatgga ccgaacgcag gaggtccgag tccctttgac ggcggccggg
     3121 ttccgcagcg aatctacatg acgggaatga tgatcgctct cggcgcgata tttatgttct
     3181 tcgtggcgct cgccagcgcg tccatcgtcc gcaagggcct tggcagtacc gattggcaac
     3241 ccttcgagct accgcgcatt ctttggctca acacactgct cctcgttgct agcagcctca
     3301 cactggcaaa tgccaggcga cgttttctgg cgcaccagtt cgcggaattc cgccactggt
     3361 ggggtgtcac gacgattctg ggtatcttct ttcttgtggg gcaaattatc gcgtggtgga
     3421 aactggcgtc ctcgggagtg tttctatcaa caagccccaa cagcggcttc ttctatgtgt
     3481 tcacggtggc gcacggtctt catcttctag gcggcaccgc aggcttgctg gcggttttct
     3541 ggtggccgcc tggcccgatg ctgcggaaga cggctactga ggtggtagcg ctatactggc
     3601 attgcatgac tgcactatgg cttggtgtat tcgtgttttt ggttgcgacg aaataagcat
     3661 gaacacgata gcccgcaaaa cggccgttcg acccaccact cggttggcca ggattttggc
     3721 ctgcgcgcta ggaatcgtcg gcctcgcatc cttccgcgtg gtcgcgcagg gttgtgcgat
     3781 gtgctaccag aacgctgctg cggtcgattc tcacggccgg accgcgcttc agcacggaat
     3841 tttaattctc ctactccctg cactcagttt tttcgtcgga atatttggcc tgatgtatca
     3901 cagacggaac acgactcgct aaagattact tggcgcaatg gacggagaag cgcaggcagg
     3961 gctaattgct cacttcagag agaacctcta ctgcgaggaa gaagggctta gacagcctcg
     4021 agctgcgtga gaaggaacgg atctcctgaa ttagtgtgga gctcgcgcca taattgctca
     4081 ggctgtgcca ggaaaacttt caccacttgc gggtcgaatt gtgttcccga gaagcgctct
     4141 atttcttctc gtgcggccga aattggcaga gccttcctat aaggacggtc ggatatcatc
     4201 gcatcaagcg tgtctgcgac agcgaaaatt ctggcgccga gtgggatctc ttcgcctctc
     4261 agtccgcgcg gatagccggt cccgtcatag aactcctgat gcgccaagac aatctccgcc
     4321 gcttccttca ggaacggaat cctttccagc acggcatagc cgatatcgca atggcggcgc
     4381 atgatctcac gttcttgcgc ggtcagggga cccggtttgc gcagaatttg atcgggcact
     4441 cccatcttgc cgatgtcatg gaggaatgcg ccgcgagcga tttgacgcag cagcgagctg
     4501 tccaccccca ttgcttttgc aatcgtaatc gtaaaagccg tcacgcgctg gcagtgtccc
     4561 tctgtctcag catctttcgc atcgagggca ccgcccagtg cttcaagtgt gtaatcgtac
     4621 gattgctcca ggtcttgcaa agcaatggag agttgcctcg tccgctcggc gaccaattgc
     4681 tctaggtcgc tctggtaagt ccggttttcc atcaccaacc tgcgatgttc cagtgcgcgc
     4741 cgcacgctca ggtaaagctg atctttctcg aatggtttga ggatgtagtc gtaagccccg
     4801 gcgcggatcg cttcaagcgc gatggaaatg tcgtgcatgg cggtcaccat aatcgctgga
     4861 acatccggat cggccattcg tagctccgcc agtagcttca ggccgtccat ttccggcatc
     4921 acaatgtcgc tcaggacgag atcgtacgaa tcttttcgaa aggtttcgag tccaaccttg
     4981 ccattcacgc aagttgtgca ctgaaaaccc tgagcatcca gcaaagtgga aatcacttcc
     5041 cggatagctt cttcgtcgtc caccacaagt atcttttcgt gggccatgtg cgctccgccg
     5101 ctgctgccca ttataaagga cggcacattc aacggttgct ctcgttgccg gatttcttgg
     5161 cgttgtgcta gtttgcacaa tggctctcta atatccccga aatctgtggc gcgacgtgcc
     5221 gttgggcggc ggacacgtca agttcgaagt gccttcaaat gacgtttgct tccaactcgg
     5281 gctttctcgc gattgccttc atggaaggct ctgctgcctt catccttttg attgtgtatt
     5341 ggctactgct cccatctttt ccggcgcgat tcttccgcta ttggctagtt ggttggaccc
     5401 tctataccgc attgcagggg cttcgcattt tttctatctg gcgcggtggt cccgatatgc
     5461 ctcattccgg ccaagccctc tctttcctgg ccgcggtctt tttcctggca tcagttctgg
     5521 actgtcggga tccaggcaag ccagtctgga aactatggcc cctctggttg tttgctgcat
     5581 cggacgtagc cgcgctcgcg tgggtcggcc ggatacatta cctgccgaac tggtccgaaa
     5641 cgctgctcga gtgttcactc tacctttcag ccggctggat tttttggggg tcgcaagccc
     5701 ggcatcgcgg cgttggatgg aagcttctcg cgggcgccct gcttctgcgg ggccttcatg
     5761 gcctggacca gccccagtgg gccgcacact ccgcggggct gttccgagtt tccttccaag
     5821 ggttattcgg aatcatgatg ggtatcgcca tggccgtcct cgtgcttgaa gcgggccgcg
     5881 gccgcaccga agacctcaat gaaaagttgc gccgcctgtc gctgatcact gcggaagcga
     5941 ctcagtctct ccgcgttgac gaagttctcc agggggtcct ctctcacctg gtcgaaagcc
     6001 ttggcggtag ccacggactt gtattcttgg tagacgacag caagcagcct cccgccctcg
     6061 cgtgccgagg ctcggttgga tttagcgaac gttttcgcaa acaacgtccc caaatttcgg
     6121 cagatgaaca gtggttgcag caattacttc tccgggaaaa gccacttacc ttgtcgctac
     6181 aaactaagaa cgtcgcccct cgttcctgga tggaaactga gaaactcgcc tccctcatgc
     6241 tcgttcgtat ccccggaaag gacgagcccc gcggcttcct agcaatcggc tccttgacgc
     6301 cccgtgattt cgagcgcgat gaacaggact tcctcgtcaa cgttgccaat cttcttggtc
     6361 tcacggcgca gaacgtcgcg ctctttcaaa acgccgccac cgctcgcagg cagtggctcg
     6421 ataccttcga ttcgatggac gatctgatcc tggttcactc actcgacggc atcatcctca
     6481 gggccaatcg cgcactggca gaccgtctgg gcgccgaact ctccactctg gtcggacgtt
     6541 cggtccgcga tgttcttcat tcctccggct ttgggtggtc gcgctgcccc tactgcgaag
     6601 gcgtcgccgg aaaagccgac gagatcgatc cttccttcgg cggatacttt cttgccagtg
     6661 attcttcgct tcacgactct gaaggcggca atctcggcac cattcacgta ctgaaggact
     6721 tgacggaccg gcgccaggct gagaacaagt tccgcaccct ttttgaaaag gttcaggagg
     6781 gcattttcat ttccacgccc gacggccggt tcctcgattt caatgacgct ttcatgcgta
     6841 tcctcggata cgaaagccat gacgaattgc tccaagccga tattcccgcg cagttgtatg
     6901 tcaaccccgc tgatcgcgat cgccgccagc gcttgctgaa cgagtatgga gaggtgactg
     6961 atttcgaatt tcagtttcgc cgccacgacg gagacattcg caccgcccac gaatccagtt
     7021 tcgccactcg cgacgattcc ggcaccgtca tcgcttacca gggctttttg cttgatgtca
     7081 ccgatcaaaa acaggccgag tttgacgttc gcaggcgcaa tcgcgccttg ctggcgctaa
     7141 ataacatcgg ggaacttcta ggccagtctc aatcgctcga ggaaggactg accgcctctc
     7201 tcgcgaaggt cgccgaactt tttgctaccg attttgggac agtctatctg ttggatgaaa
     7261 ctagcaattc acttaagcga gccgccgcca tcggttgccg ctcgaatcag gccgcgcggc
     7321 ctgagccaat tgccattccc gcggcgcttc tggaacagat acgccaggct catgccaccc
     7381 ttctatccgg atcccttccc tcgctgccgg aggccctgcg cgaattccac gagcgcgaag
     7441 gcatacaatc ttctcaactg gccatgcttt gggccaagga cagcgtggtc ggcgccctcg
     7501 tcatcggcag ccgcgaggca cgcgcctttt cgacggccga attgaatttg ctctcggccg
     7561 tcggcaatca gatcgccacc acgattgaca aatccctttt gctcgagaaa actcgcgaag
     7621 cttacgattc attgcgccgc acgcaggagc agttgctgca aagcgaaaag atggccgctg
     7681 tcggacaact gatttccggc gtggcgcacg agctcaacaa ccccttaacg gccatactcg
     7741 gttataccca gctgctgaaa tccgacgaac ttgttctacc gcgcgggctg gattatgtcg
     7801 aaaaactcta taaacaggcg cagcgaactc accacattgt ccagaacctg ctcagttttg
     7861 cccgccaaca gaagcctgag cgtgcctcgg ttcaaatcaa tcagatcctc gaggaaactc
     7921 tgatccttcg ggaatttgac atgaagtata acaatatcgt gattcggcgg gaatttgacg
     7981 cccagctccc acccaccggt ggcgactttc atcagctcga acaagttttt ctcaatattc
     8041 tcaacaacgc cgtggatgcc attcaggaga caggcgcgcc gggggaaatc accattcgaa
     8101 ccgaggtcgc cgggaatcgc ctgtgtgtcg agtttagaga caacggtccg ggtgtcacca
     8161 acccgcaccg catttttgat ccgttttaca ccacaaagcc cgtgggaaag ggaacaggtc
     8221 tcggtctcag catctgctac ggtatcgtga aggagcacgg cggtgaaatc cgcgttcgca
     8281 acgtggaacc tcgaggtgcc gctttcaccg ttacgctgcc cttgctggtg gtcggtccct
     8341 cggagccggc agagctagca tcgcccgtct ccaccgtctc cggctcctaa gaaaccgtgc
     8401 ccgcgtctgc cgcgctttct gttgtgctta ttcgggactg aatgccaaca gcacgttgcc
     8461 cggcattcca aagagcgcgc cgtatccgga atttacgaat accatcccgc ccgcaattgc
     8521 cgcaccgccg cccccgtcga ttgacccgcc gtgcgccgga accttgttca cggtcttgaa
     8581 gtcgtgcgcc gtatcgaagt cccactcaat ctttccgttc tcggccgaat aggcccgcat
     8641 gtggccgtca tccgaacccg agaaaaccac acctggcatg gccgtcgctg cggccgattg
     8701 ggcgggcgtg catcgcgcaa cgtggcaggt tcccaacacg ggaggagcag tccatatccg
     8761 tttgcccgtt gcaatatcga gggcgaataa tcccccgcct tttgtggaat cgcccttgat
     8821 cccttcgggg aacgaaatta cgtccaggtc cgataacgcc tcgtatacca ctttggcgtc
     8881 cgccgcaggc ccccattcta ttccaccaag cggtcctccc ttgccgattt gggccttcca
     8941 gaggatcgct ccttgcttgt ctggatcaat ggccagcacc gtcgcggact tttggctcag
     9001 gatcagcacc cgctgcccgc tcggaagcgg gcgcaaaagg ggcggagcgg cgaagtcata
     9061 atcgccgccc ggtttggccg ggcaattaac catatttttt tgaatgcagc tggtgttgaa
     9121 cgcatctccc gccgtgagct gttttgacca aacgattttt cctgccttca tatccaccgc
     9181 gagaattgaa tcgctaagcg gcgtcggcgg atcggaatag ttgtcgccga cccccacgta
     9241 aagaagtccc tttttcgcgt ccaccgttgg cgatgaccat atcgccgcgc cggaaggtcc
     9301 ccacctccgc gttccgtctg gcttcgtcgt agtcggctgt ggcggggtcg aaatcgtgta
     9361 agtcttccaa atttgctttc cggttgcggc atccagcgag accaggactc ctcgaaaagt
     9421 gcagcattcg taatgtgggt cggatgccat ccattcctcg cgcgacgata caccggcgta
     9481 aagccgcccc tcgtagagct ttggcgcatc cgtcacacgc gcgtacggat gatcgctgat
     9541 gcgggttttc cagacgagct ttcccgtaat cgcattcacc gcgtaaatat ttgtccggta
     9601 atcgccgaag taggccaggt ttcctggacc cagcaccacg gcgctccgaa ctgcaccttc
     9661 ggcgcggaat ttccaatacg tgcatcccgt tttctcgtcc agcgcataga ccatccccat
     9721 tgagctgccg acgaaaaccc gcccgcccgc gatcaccggc tgcgcatacg cgccccaatc
     9781 ggccggaaag ccaaacgccc atttcagttt tagctttggc acctgagccg cactcaggcc
     9841 ggcttgatcg gcaggttgga accggctgtt gtccatgtcc acgccccagc cgttccattc
     9901 gggtccggaa aaatctgaag atgcccggcc tgtgcactgg cccgcgagcg gattcgtcgg
     9961 ccgtgaactt cccgcgcgct tgccggtcag atattctgca accgccacgc gctggcgaaa
    10021 aggcatattg gcggcaagct cccgcattgc tcccgatctg agaacgtaga ggatatagtc
    10081 cggcgacatg ctcttcagga tgtaatgatt gaagagcgga gccgttccca cctcgtggca
    10141 ggaggcgcac ttgctggcat atattgccgc gccttccgcc tctggcgagt tcgaagccgt
    10201 cgcgggacgc gcttcggcgg cgctctcgcc gttcttcttt agtgaaagca catagtccgc
    10261 gagatcacca atctgctccg aactatagtt gcccttgaat gcaggcattg ccgtgcccgg
    10321 cttgccctct gaaattatcg tcgttatttt ctcggcggat aaatcgagtt tgcggaggct
    10381 gaccgccgcg ccgctaccgt ttccatccac gccatggcag aagacgcagc ctgatgcgta
    10441 gagtttcttg ccggcttgga ttcgcttcgc gacacctgcg gttgtcgagg tattttgtgc
    10501 agtggccagg tggatcgccc cagcggaatg gaaaccgaaa cagagtacga ggaaagccgt
    10561 cgcggcgcga accgcgctat tcatgcagat cgtccgtgac ttcaagtccg cgcgccatgc
    10621 gatgtctcca ctggaccgct ttccgtcgtg ccaagataaa gattgccacg gtgcgtgtca
    10681 atcgcacagc gcagcaccca tgcctcattt caactggttg tgcaccgcca cgaggccaag
    10741 catcagcggc agcagcaaaa tcagcgccat gatgatccac gccgaaacat gggcaaaatt
    10801 catggtgtac ccgatgccgg cccgcttttc gacccagagt gccgcatcgt ccggattgaa
    10861 atagagcatt ccgaacttcc aacattcgtc gggcgtgcca tccccggcgg ctgtcggatt
    10921 cgtctcgccg gacctttcaa tatgtgcgcg ccctttgttc agccacacga tgagaacgaa
    10981 aaccgccggc agcatcagaa tggtcacaat caacactggc atcgcgccgg ccagcggcag
    11041 cagtcccaca cacgaaaaga tcaccgccag aaaaaattcg atcagcagga gaaacagtcc
    11101 cacgcgatgc gcgaaatcgt gaagcaccaa gaccggtctc gcgcgatcgg taatgcgcgc
    11161 ggatcgaagc agtgtattgg ccgtcagcca catgcacgcc gagattaggg ccccgaaaag
    11221 cagtggggcg tacaccccca tctgagtgcg gctggaccac ccgttcggtc gcccgtcaat
    11281 tccccagtgg acggggaagc gcgccggtat ttcatcccag tgaaaatgaa gaaagaggcc
    11341 cacggcgagc aaaatcgcaa acggtcccac ctgcacgaac cacccgcctg gcgggcgcgt
    11401 gttgcgcggc tcgagtgtcg cttcgcggac ggttacagct cgcacggcat aacgcttcac
    11461 ccgcttgtga gcttctacga acgccgtaag gggtccgacc gccagccaca atccgccaat
    11521 gatcgggaga aagctatatc ccggtagcgc acctacaaga accatcccaa aagcaatcgc
    11581 cacatgaatc atcgcttcaa gtctgtacct ttgaagcagg cgttcagctt ccggtgtgtt
    11641 cccgaaatcc ggtgccaccg tgacgccaaa ataaattccg ggccgcgtcc attgcggcag
    11701 catccaaaat gcccaggtca ccagcgccgc cagtgcgagc aagaatgggc cgaaaatcca
    11761 ttcggaatgc tgaatcatct cgtgcctcca tggcccagtt tttttgctat atccgccatg
    11821 cgacccgcga taactgaccg cggaactcca ttcgtgatgg ccttggcgat cagttcttcc
    11881 agatgctttc ggaaatcctg cttcgcctca ggagtcgcct tcggatcacg ccggtcgagg
    11941 acaaccgctc cgcgacgcct ccgcaaatcc agccagcctt cctccgccag tattcggtac
    12001 gcctctgcga ccgtattgtg atgaacgccg agatcgatcg ccagtcgccg taccgtggga
    12061 agttgctccc cggattcgaa cgcgcccgaa actagcaacg cacgcagccc gcttacaatc
    12121 tgctcgtaaa ccggctgcgc ggcggccaaa tcaatgtgta atgtgcgtgg gcttttcatt
    12181 gcctattgta catgtacagt atacaaaagc gagcctcggg tcaagtccga cgccctggac
    12241 cgggtaacga tgtcgcggcc cccccggctg ccgcatcggg tttgccggag cgcaccgctc
    12301 ttacccccgt cgcgctcagg ctatccaaac ctgctatcct tcttgcgcgg ccatatttcc
    12361 gggaggcggg aatgaagcga tggatcggct tgctgtttgt ccttgcggtc ggaattacgg
    12421 cggcagcgcg ccaatccgca tcgccgtccg cagcgcttcc gttaaattcc gtttcctccc
    12481 aggctccagt agctccgaat cccgaatttc tgaagaccgc cgacgaagtc ctgcagcaga
    12541 tgagcgttat tcttcacctt cccatcgacc agcctctcaa gaaaaccctg cgctcgaagc
    12601 aggagattcg cgactacttg attcgcgagg aaaaggaaga caaaaacgaa gcgcagcgct
    12661 acgccgatac gaagtctctc gaagctttcg gcctcatccc caaaggattt cccctcgatt
    12721 cgttcatgct cgatgtcctc accgatcagg ttgccggtct ttacgatccc aaagcgaagg
    12781 agttctatat cgcggattgg atcccagcga gcgagcagcg agaggtgatg tcgcacgagc
    12841 ttacgcacgc tcttgaagac cagtccttta atgtggaccc ttggatcaag gccgcacggc
    12901 cgaatgacga tgccgaaatg gcgcgcgacg cggtcagcga aggcaccgcc atggctgcca
    12961 tggtcgatta cgcgctgcgc gatcagaatg tcggcgtccg cgatctggtt gacgtcagca
    13021 tgctgattcg cgcgggcgcc gtcgcggaaa tgaacaacga cccaaaactc tcgaaagcgc
    13081 ccctgtacat tcgcgacggc cttttgtttc cctatcttgc gggcaccagt tttgcgcagc
    13141 agtttctgaa ggcacacacc ggctggtcgg accttcatct gcttttcgag catccgcccg
    13201 tttcgacaca acagattctt catcccgatt tttacctgaa ggatgtggga cccgctaaag
    13261 tcacgcttcc ggcctggaag ggtttggttc ctgcggactg gaagttgctc gaggaaaatg
    13321 taatgggcga attcggcctc gaggaagtgc tcaagcaatt tctggatccg gcgcgggccg
    13381 ccgctctttc acctgcatgg gatggcgacc gttatgccgt cttcgaggac acgaaaacga
    13441 aggaaacgcc cttggtgttc catctggcgc tcgaaactcc ggacgatacg gcgcgtttct
    13501 tcggacaata cagcgaggct ctggaaacga aatacaaaac acgcaccgca ctttaccgcc
    13561 gtcccaattt tttccagttc cagacggatg cgggcggcgt cttccttcgc tgcatggagt
    13621 tgcagtgcct gagtgtcgaa cgcgccacgc gcgcgacctt cgacacaatc aatcgcgcca
    13681 tcggctggcc cgccgcgcca ggcccagtag aagctgagcc gtcggtggcg cagctcagag
    13741 aagccaagcc agccgaagct acgcgggcgc cacgatctca ctgaaaacca aatattttcg
    13801 ccaatgagca ttttcgaatt acagcgcgtc gctgaacccg aagtgatgga cgattccgcc
    13861 gaggtggaag catattcttc ggcagccgcg caatctcacc tgaacgacat cgacgacacg
    13921 ttcgttgacc acgccacaag gttggttaac aaacgcgaaa gaggccgcgc gctcgatatt
    13981 ggcaccggtc cagggcaaat cgtcctgaag cttgcgcgcc ggctcacgcg atggaagttt
    14041 attggagtgg accggtcgcc caacatgatc gcccagggca ttgccggcct ggcgccttca
    14101 acggaagttg ctggccgcgt tcatttctat atcgctgacg gaaatcagat tccgttttgc
    14161 gatggaagtt ttgatttggt ggcgtgcaat tccgtgctgc accattttgc cgatccgcaa
    14221 aaattactgg ccgaaattgc gcgcctcgcg aaaccggaaa gcgcgatcct gctccgcgat
    14281 ctccgaaggc cgagtcgctt ggcgtatccc ctccacgtcc ggcggcacgg acggcattac
    14341 caggggctga tgtacaagct ctactgcgat tccgtgcgct cggcgtacac ggtcgaggaa
    14401 ttacagcggc tgctcggctc ctctccgctg aagggcgcgc gaattttcga gcacaaccac
    14461 acgcacatcg gaatcgagcg ccccatctcg tcgtaggatc gcgccctagt cggttagtcg
    14521 ttgaaagatg agtggccgga ttgggctcgc tgaatcgcca tcaaagtctg gtcgtccgct
    14581 tgccgcgtcc cggatgaatg tctttccaaa tctttcatgc aagccgcaac cagcgcttcc
    14641 ggcgtcaaac cgtgcttttc gctggccgcg attgaaagtc cgctgatccc atattcattt
    14701 cctgaaggat cgcgcgcttc cgaaatgccg tcggtataga gcagcagact gtcgtcaggt
    14761 tcgaggcgcg cgcggcgaac agcgtagcta ctggtcgtaa acattcccag cggcaggccc
    14821 gtagcctcga tgcgactgac gccgtttttc ccgagcagca gagcagggaa atggcctgcg
    14881 ctggcgattt cgacttcgcc gtcgcggccg attcgcccgc acaccagagt agcgtactgc
    14941 cctgcgatgg tgctttcgca gaagaggcga ttcgccattt ccagcagctt atcgagttca
    15001 aggccgatgc ccgccaggct gcggaacatc gcgtgcaagt gggtcatcag cagtgacgcc
    15061 gccactccct tgcccgtcac gtctccaaga aggaaaatca ctttgccgtc gcccgtggcg
    15121 gagggaatca cgtcgcaata atcgccgctg acgacgcccg ctggcttgta ccggtagtga
    15181 atgcgccagt cctgaaatcg cgtgtccgtt tgcggcagta atccccgctg gatgcgcgca
    15241 gcaagctcga gatcgcgctc aagcgctcgt tgctcctcgc cggtcaaaca gtcgaggcac
    15301 atctgagcca aaggatccga aatcagccgt tcgctcccca gagtatcgtg gcaggcctcg
    15361 cagattccgt aggttccctc gtccattcgc tccattgcgg aatcgacttc ctgcagtaga
    15421 tcgagcagag gagtggcggc ttccggatca tccacaggga tcacggcaat gtttgtcttc
    15481 aactccgcgc gccttttctc caattgctcg cgcagataag gatcggtgat tccagacgcg
    15541 gctttgttct ccggctccga gattctcgcg
//
LOCUS       DATNDT010000139         7983 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3799287, whole genome shotgun sequence.
ACCESSION   DATNDT010000139 DATNDT010000000
VERSION     DATNDT010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7983)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7983)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7983
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3799287"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..813)
                     /locus_tag="VN788_04405"
     CDS             complement(<1..813)
                     /locus_tag="VN788_04405"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HXU19805.1"
                     /translation="MQLQEKYVRADKLRGSVCFQRDWKSCSPGTRDRRWIARTLYLTF
                     ALIAVCALLPGHAWSSQRSESARRASASRHSARTRAKRKHIGLQISKINDPNNRETVG
                     AHSHGDAVVRAAILLDRLKLSPGEISKSYDDNLKKAIQAFQSASGLPSTGEVDSATWA
                     ALNSDQGTKGEVEQKQGGPEQNGPNPQQNNAPQNQTSGTKPQPQPNSAQGLGQSSGQG
                     QSQTGKPQQPAQPQSDASQTEALTTYIIAFEDVAGPFTRLPRIRGRDSGERRM"
     gene            1063..1902
                     /locus_tag="VN788_04410"
     CDS             1063..1902
                     /locus_tag="VN788_04410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006889744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19806.1"
                     /translation="MSSPVRANSTIQQFGGVSASNEAYASAISWSAVIGGAFAAAALS
                     LALLTLGAGTGLSAISPWSSRGASASAVGIGAIIWLIVIEILASAMGGYLAGRLRTKW
                     VNVHTHEVYFRDTAHGFLVWCVGLVITAAFLATAAASVVGATGNATAIATTEGQTGPN
                     AYFVDALFRSDRAERVDPSERTEAGLIIARGIQEKNLPTADRTYLAQRVSARTGLSET
                     DAQKRVSEVFDQARQNADTARKAAAHSLYWTFLALLIGAFCASFAATIGGRQRDRMAA
                     ATI"
     gene            complement(2059..3717)
                     /locus_tag="VN788_04415"
     CDS             complement(2059..3717)
                     /locus_tag="VN788_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014194029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HXU19807.1"
                     /translation="MINDLWYKNAIVYCLSVGTYLDTNGDGVGDFRGLLRRLDYLQGL
                     GVTAIWLLPFQPTPGRDDGYDVSDYYGVDPRHGSLGDFVEFTQGCRQRGLRVLIDLVV
                     NHTSDQHQWFQEARKDPKSKYRNWYIWSKKKPRNANKGMVFPGVQKSTWTYDDAAKAW
                     YFHRFYDFQPDLNTTNPEVQAEILKIMGFWLQLGVSGFRMDAVPFVISTKGVANRQST
                     KHYDMLRMFREFLTWREGDAIILAEANVLSDVDMDYFGTSGERLQMMFNFEVNQHLFF
                     ALATGDTRPLTEALKTTKPRPANAQWAQFLRNHDELDLGRLTSKQRQAVFAAFGPEPN
                     MQLYKRGIRRRLAPMLQGDRRRLELAYSLMMTLPGSPVFRYGDEIGMGDDLSLPERDC
                     ARTPMQWSSEPHAGFTESDKPVLPVISEGPYGFEHVNVAAQKRDPNSFMNWTERMIRM
                     RKEVPEIGWGDFSIVATRKPEVFALRYDWRNNSVLFVHNLSPIPTEVEFGAGTKVDGQ
                     LVNVLSDDHSTPDAGGKHRMVLEPYGYRWFRIGGLDYLLKRTEF"
     gene            3833..5425
                     /locus_tag="VN788_04420"
     CDS             3833..5425
                     /locus_tag="VN788_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018748607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HXU19808.1"
                     /translation="MQDLKWWQRGIIYQIYPRSFQDSNGDGIGDLPGIIQRLDHLVTL
                     GIDAIWISPIYPSPMADFGYDVADYRDVAGIFGTLADFDRLVAETHSRGLRLILDFVP
                     NHTSNEHPWFLESRSSRHNPKRDWYIWRDPPAEGALPNNWLSDFGGPAWEWDETTKQF
                     YYHTFLRQQPDLNWRNPAVKHAMYDVLRFWLNRGADGFRVDALWYLIKDDQFRDNPVN
                     PGYVPGQPSSNRVLPFYTANRPEVHPIVAEMRSVIDAYPDRVLIGEIYLPIKQLVTYY
                     GSNLNGANLPLNFQLLRTAWNAESIARIMEDYEAALPQGAWPNWVLGNHDNTRIASKI
                     GAAQARVAAMLLLTLRGTPTIYYGEELGMKDVLIPPEQVHDPAEKNEPGLGLGRDPER
                     SPMLWDGSPHAGFTSGEPWLPLEADHASVNVAAFRKVPASILNLYRRLIELRRAHAAL
                     TQGRITAVGVQGNILRYERCLGNERLIILLNLGHQEAQVPHPSGRLLLSTYLDRVGKE
                     AVESTLIRTNEGIILQANCESP"
     gene            6016..6570
                     /locus_tag="VN788_04425"
     CDS             6016..6570
                     /locus_tag="VN788_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="PEP-CTERM proteins occur, often in large numbers,
                     in the proteomes of bacteria that also encode an
                     exosortase, a predicted intramembrane cysteine proteinase.
                     The presence of a PEP-CTERM domain at a protein's
                     C-terminus predicts cleavage within the sorting domain,
                     followed by covalent anchoring to some some component of
                     the (usually Gram-negative) cell surface. Many PEP-CTERM
                     proteins exhibit an unusual sequence composition that
                     includes large numbers of potential glycosylation sites.
                     Expression of one such protein has been shown restore the
                     ability of a bacterium to form floc, a type of biofilm;
                     Derived by automated computational analysis using gene
                     prediction method: GeneMarkS-2+.
                     GO_component: GO:0031240 - external side of cell outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU19809.1"
                     /translation="MGKSIWIILATLLLAVCAPSAHADIISTFDASGTFGDGSALTGT
                     ITIDTSNGLVTATDLFVGSPGNVGPFTSFVQSLPSGQTAIDTTGAGGSELFLMLGAGS
                     LVGYSGSDLCSTTEVCTVFGAFSFYAEGGMLVSALSSGDLTEAGTGVNTPEPPSMFLL
                     IAGLLGLAGALRHKPLDGRLRRCA"
     gene            complement(6750..7850)
                     /locus_tag="VN788_04430"
     CDS             complement(6750..7850)
                     /locus_tag="VN788_04430"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXU19810.1"
                     /translation="MHVGYGTGFQHLGGYSDTKFMREELGNCEAAESRGFDSVWVTEH
                     HFDNYSLSPDVCQLLSYVAGKTKRIKLGSMVIVVPWHDPVRLTEQISLLDHLSGGRYI
                     LGVGRGLARREFEGLRINQNEGRMRFDEYTKMVLDALESGFVEGGETLKQPRREIRPR
                     PYKSFKGRSFAAAASRDSVPVVAKLGLGLLVILQKAWDQVAADFETYHKHWREFHPNT
                     EPPKPFCSGFCFVDESADRAEELARKYLAANYREALNHYEMFGKHFDSTKGYERYADL
                     SKYLNSIGADKAVEDYIQLMPWGTPQQVLDKFSKMKDLTDMQGIMPNLYFGGMPYEES
                     TRNINCFVKHCLQELKSWPAASLKEPAELTAA"
BASE COUNT         1622 a         2275 c         2235 g         1851 t
ORIGIN      
        1 cattcgccgc tctccactgt cgcgacctct tattcgtgga agccgagtga acgggcctgc
       61 cacatcctca aacgcaatga tgtacgtggt gagcgcttcc gtctgagatg cgtcgctctg
      121 gggctgtgcc ggttgctggg gttttccggt ttgcgattgc ccttggccgg aactctgacc
      181 aaggccttga gccgaattgg gctgcggctg tggcttagtg ccagaagttt gattctgcgg
      241 cgcattattc tgctgcggat tcggcccatt ctgctcgggg ccgccctgtt tttgctccac
      301 ttcacctttg gttccttggt cggaatttag agcggcccag gtagccgaat ccacctcgcc
      361 agttgacggc aatccgctag ccgactgaaa tgcctggatc gccttcttta aattgtcgtc
      421 gtaactttta ctgatttcgc cgggagagag tttcaatcgg tcgagcagga tagccgctct
      481 gacgaccgcg tcaccgtggg aatgcgcacc tactgtctcc cgattatttg gatcgttaat
      541 cttcgatatt tgcagcccaa tatgtttccg ctttgcccga gtccttgccg aatggcgcga
      601 tgcgcttgcg cgtctcgcgg attctgaccg ctggctgctc caggcatggc cgggcagcag
      661 cgcacaaact gcgatcagcg caaaagtaag atacagagtt ctcgcgatcc aacgccgatc
      721 ccgggttcca ggcgagcagc ttttccagtc gcgttggaag cagacactcc cacgtagctt
      781 gtccgcgcga acatattttt cttgtagctg catatgcggc cgcaaaggag aagggcgata
      841 gcgggtcggg tgaaacaaac cgccttcagt atggtagtag caggctcgat tcctcttcca
      901 tatggggaac tatctactcg gaaggtgggg aattctgtag tcggggccag cctcgatgac
      961 cgcctcccgc cgggtacagc aacttcggcg aaggggtagg cgttcccaag tatgtccaac
     1021 cggcccattg ggcaatacct tcgagtgcca gaggtaactc caatgtcaag tccagtccga
     1081 gcgaactcaa ccattcagca gttcggtggc gtctctgctt caaacgaggc atacgcatcc
     1141 gctatttctt ggagtgctgt aattggcggc gcgttcgcgg cagccgcatt gtcgcttgct
     1201 ttgctcacct tgggcgctgg aaccggctta tcggcgattt cgccctggtc gtccagaggc
     1261 gcgtcggcat cggcagtcgg tataggggcg atcatttggt tgatcgtcat tgaaattctc
     1321 gcttccgcaa tgggcgggta tctcgcggga cgtctgcgca cgaagtgggt taacgtgcac
     1381 acccacgaag tatattttcg agatacagcc cacggttttc tcgtgtggtg cgtcggcctg
     1441 gtgatcacag ctgcttttct ggcgacggcc gccgcgtctg tcgtaggcgc cacggggaac
     1501 gcgaccgcca tagctacaac cgaaggccaa actggtccga atgcgtattt tgtggatgcc
     1561 ctgttccgtt cagacagggc agagcgggtt gatccttccg agcgcacgga ggcgggattg
     1621 attatcgcca gaggaattca ggaaaagaat ctgcccacag ccgaccggac gtatctggct
     1681 cagcgtgtat cggcgagaac cgggctcagc gaaacggacg cgcaaaagcg ggtgtccgaa
     1741 gtttttgacc aggcgcgaca gaacgcagac accgctcgca aggcggcggc tcactcgctc
     1801 tattggacgt ttctcgcgct tctgatcggc gcgttctgcg ccagctttgc cgctaccatc
     1861 ggcggcaggc aacgcgatcg tatggctgct gcaacaattt aaatactgca aggaggcttc
     1921 gccatgcgtt ccatcttgct gcttttgctc ggcgtaccga tacccatcat catcttaatc
     1981 gggattttta gtcacttctg attttcaagc gtctcgaact gccaagcgtg cggtagagcg
     2041 aggcacgcca taagccagct agaattccgt gcgttttagc aggtaatcca ggccgccgat
     2101 gcggaaccag cggtagccgt agggctcgag caccatgcga tgtttgccgc cggcatcggg
     2161 cgtgctgtga tcgtccgaca gtacattgac gagttggcca tccaccttcg tgccggcgcc
     2221 gaattcgact tccgtgggaa tggggctcag attgtgcacg aatagaacgg aattgttccg
     2281 ccagtcatag cgaagcgcaa acacctcggg tttgcgcgtc gctacgattg aaaaatcgcc
     2341 ccaccctatc tcgggaactt cttttctcat gcggatcatg cgctccgtcc agttcataaa
     2401 agagttgggg tctcgcttct gcgcggccac atttacgtgc tcgaaaccgt acggaccttc
     2461 ggagattact ggaagtaccg gcttatcgct ttccgtgaac ccggcgtgcg gttcacttga
     2521 ccattgcatc ggagtgcgcg cgcagtcgcg ttcgggcagg ctgagatcgt cgcccatgcc
     2581 gatttcgtcc ccatagcgga agacggggct tcccggaaga gtcatcatca gactgtatgc
     2641 cagttcaagc ctgcggcggt cgccttgcag catgggggcg aggcggcgtc gaatcccccg
     2701 cttatacaat tgcatgttgg gttcggggcc gaatgcggcg aacaccgcct ggcgctgctt
     2761 gctcgtgagg cgaccaagat caagctcatc gtggtttcgc aagaattggg cccactgcgc
     2821 gttcgcgggg cgcggcttcg ttgttttcaa agcctcagtc agcggacgag tgtcgcccgt
     2881 ggccagcgcg aaaaaaagat gttgattgac ctcaaaattg aacatcatct gcaggcgctc
     2941 gccggaagtg ccaaagtagt ccatgtccac atcggagagc acgtttgcct cggcaagaat
     3001 gatggcgtcc ccttcgcgcc atgtgagaaa ttcgcggaac atgcgaagca tgtcgtagtg
     3061 cttcgtggat tgtcgattcg caacgccctt ggttgaaatc acaaagggta cggcatccat
     3121 gcgaaaccca gagacgccta actgaagcca aaaccccatg atcttcaaaa tctcagcctg
     3181 cacctcggga ttcgtagtgt taaggtcggg ctgaaaatcg taaaacctgt gaaaatacca
     3241 ggctttcgcc gcgtcatcgt acgtccacgt cgatttctgg acgcccggaa agaccatgcc
     3301 tttgttcgcg tttcgaggct tcttcttaga ccagatgtac caattgcggt atttggactt
     3361 agggtctttg cgcgcctctt gaaaccactg gtgctgatcc gaagtgtgat ttaccaccag
     3421 atcgatcaat acccgcaggc ctcgctgacg acaaccctgc gtaaactcga cgaaatcgcc
     3481 taacgatccg tggcggggat ccaccccgta ataatcggaa acgtcgtagc cgtcgtcccg
     3541 tcccggcgtc ggctgaaacg gaagcagcca gattgccgtc accccgaggc cctgcaggta
     3601 atcgagccgc cgcagcaggc cgcgaaaatc gccgacgcca tccccgttgg tgtcgagata
     3661 ggtcccgacg gagaggcagt aaacgatcgc atttttgtac cagaggtcgt tgatcatccg
     3721 cggtcctcgg gatattcctc agtgacgcct tgcggatcga ttctaccttg agccatattc
     3781 gttttgacgg gcgatctagt tgtgctggcg atctcttggg ggcctcgagc acatgcagga
     3841 tttgaaatgg tggcagcgtg gcattatcta tcagatttac ccgcgctcat ttcaggatag
     3901 caatggtgat ggaattggcg atcttccggg aattatccag cgtctggatc atctcgtaac
     3961 gcttggaatc gacgccattt ggatttctcc aatttatcct tcgcccatgg cggattttgg
     4021 atacgacgtc gcggattatc gcgatgtcgc gggaatcttc ggaacccttg cggatttcga
     4081 tcgtttggtc gctgagacgc attcgcgagg gctcaggctc attctggatt tcgttcccaa
     4141 ccacacgtcg aacgagcatc cctggtttct tgaaagtcga agctcgcgcc acaacccgaa
     4201 aagagattgg tacatctgga gagatcctcc tgcagagggc gctctaccca ataattggct
     4261 gagcgatttc ggcggccctg cttgggaatg ggacgaaacg accaaacagt tttactacca
     4321 cacgtttttg cggcagcaac cggacctcaa ttggcggaat cccgcagtta agcacgcaat
     4381 gtacgatgtg ctgcgatttt ggcttaatcg tggcgcggat ggctttcgag tggatgcgct
     4441 ctggtacctg atcaaagatg atcagtttcg ggacaatccc gtgaatcccg gctacgttcc
     4501 ggggcaaccg tcatccaatc gcgtgttgcc cttctatacc gccaatcggc ctgaggttca
     4561 tccgatcgtt gccgagatgc gcagtgtgat cgatgcttat ccagatcgcg ttttgatcgg
     4621 tgagatttat ctgccgatta agcagttggt cacctattac gggtccaacc tgaacggcgc
     4681 gaatttgccg ctcaactttc agcttctgcg gactgcgtgg aacgcggaga gcattgctcg
     4741 gatcatggaa gactacgaag cggcgttgcc acagggagct tggcccaact gggtattggg
     4801 aaatcacgac aacactcgga tcgcgtcgaa aattggcgcc gcgcaggccc gcgtcgcggc
     4861 aatgctgctg ctgaccttgc gcggcacgcc aaccatttat tacggggaag aacttgggat
     4921 gaaggatgtt ctcattccgc ccgagcaggt tcacgacccc gccgagaaaa acgagccggg
     4981 gctgggattg gggcgcgatc cggagcgatc cccaatgctg tgggatggat ctcctcatgc
     5041 aggttttact tctggagagc catggctgcc tttggaggcg gatcacgcga gcgtgaatgt
     5101 ggcggcattt cgaaaagtgc ctgcatcgat actgaatctt taccggcggc tcattgaatt
     5161 acgacgagcg catgcggcac tgacgcaggg gcgaatcact gcggtgggtg ttcagggcaa
     5221 cattttgagg tacgaaaggt gcctggggaa cgaacgactc attattctgc tcaacctcgg
     5281 tcatcaagag gcgcaagtac cgcatccatc cggccgttta ctactatcca catacctcga
     5341 tcgcgtcgga aaggaagcag tagaaagtac gttaattcgc accaacgagg gaatcatcct
     5401 tcaggccaac tgcgaatctc cataaatttc cagctgacga gtacctcgtg ttgaagagga
     5461 aaatgccggt ccgggcaccc aggacccgtt tcgcgaacaa gtttccccca tgatttagag
     5521 agactgacgg tagcccaggt gctgtatctg ttttgaaatt taccggtgac gccgcgctgc
     5581 gttaggactg ttgtgctcgg aatcgcggat ggccgcgagt ccacgccacc agtcggcttg
     5641 gtgccggcag cgccggctcg ccctgaggaa cgaagggagg cgcgattgta attagcccgg
     5701 cacgcagcga agcgaagtgc cgggatacgc ttcattctca ttgcgcgcgc gactcttccg
     5761 gccggggtgc ccaaaggcat ctcgggcgcg cagccttacg ctcgcgcttc acccacgcga
     5821 gccttcagct tcgtgtctgt gaacgtgtgc gtttccccgg tccctacgcg gacaacttag
     5881 gcttttgtgc caaagccgcg gcatggtacg ggtactcaca aacactattt tcttcttgca
     5941 atcaggcggc aaggcctcat actcccctca tttcggcccg aatcgccaga aaattccgtt
     6001 gggggaaaat cgattatggg aaaatctatt tggattatcc tcgccacact gctcctggcc
     6061 gtttgtgcac ccagcgctca cgccgatatc atttccacgt tcgacgcatc cggcacgttc
     6121 ggagatgggt cagctctcac tggaacgata acaatcgaca cctcaaatgg gttggtaact
     6181 gcgaccgatt tgttcgtggg gtcgccgggg aatgtgggac cattcacctc attcgtgcag
     6241 tcgctaccct cgggacagac cgcgatcgat acgacaggag cggggggttc ggaacttttc
     6301 ctcatgttgg gggcgggctc gcttgtcgga tacagcggca gcgacctgtg ctcaaccacc
     6361 gaagtgtgta ccgtctttgg tgccttttcg ttctatgcgg agggcggcat gcttgtatcg
     6421 gcattgtcta gcggagacct caccgaggcc ggcaccgggg tgaacactcc agagccgcca
     6481 tctatgtttt tgcttatcgc cggcttactg ggcttggccg gcgcattacg ccacaagcct
     6541 ctcgacgggc gcctcagacg ctgcgcctga ttttgaattt gagcttcccc atcgtcgccg
     6601 gttctgagag gggtggggtt ttctatatct ctccccgctc gacggcgatg agttggaacg
     6661 accgccttag tcgctcaatc tgggcttgct cccctcgtcg ccgcgtgctt caagcctcag
     6721 tacaaattag cggcggcacc tcgcagaacc tacgcggcgg ttaattccgc gggttccttc
     6781 aacgacgccg cgggccagct tttcagctct tgcaggcagt gcttcacaaa acaattgata
     6841 ttccgagtgg attcctcgta cggcattcca ccgaagtaca agttcggcat gattccttgc
     6901 atatccgtta gatctttcat cttggaaaat ttatcgagca cctgctgcgg agtgccccag
     6961 ggcatcagct ggatgtaatc ctccaccgct ttgtccgccc cgatcgaatt tagatacttg
     7021 ctgagatcgg cataccgctc gtagcccttt gtgctgtcga aatgcttgcc aaacatttcg
     7081 tagtgattga gcgcctcgcg ataattcgcc gccagatatt tgcgcgcaag ctcttccgcc
     7141 cggtccgcgc tctcatcgac aaaacaaaag cccgagcaaa acggcttcgg cggctcggtg
     7201 ttgggatgaa actcgcgcca gtgcttatgg taggtttcga aatccgccgc cacctgatcc
     7261 cacgccttct gaagaatcac gagtagcccc aatccaagtt tcgcgaccac gggaaccgaa
     7321 tcgcgcgacg cagccgctgc gaaagatctt cccttgaatg acttgtatgg ccgcgggcga
     7381 atctcgcgac gcggctgttt caacgtctcg ccgccctcga cgaatccgct ttcgagcgcg
     7441 tccagcacca ttttcgtgta ctcatcaaag cgcatgcgcc cttcgttttg gttgatgcgc
     7501 agcccctcga attcccgtcg cgccagtcct cgtccaacgc ccaaaatgta gcgccctccc
     7561 gaaagatggt cgagcagcga aatctgttcc gtcagccgca ccggatcatg ccacggcacc
     7621 acaatcacca tcgaacccag cttgattcgc ttcgtcttgc cggccacgta agaaagcaat
     7681 tggcaaacgt cggggctgag cgagtaatta tcgaaatgat gttccgtcac ccaaacagaa
     7741 tcgaagcccc gcgattccgc cgcttcgcaa tttcccagtt cctcgcgcat gaatttcgta
     7801 tccgaatagc caccgaggtg ttggaagccg gttccgtaac ctacatgcat gatgtcatcc
     7861 ttcctgtttt cttcccgcgt gaatcaaaat ttcggcgggt tcgccaagca gcgttcgcgg
     7921 cggcggggac tgtatcagat tactccagac ccggtcattc gcgtcaaggt ggcgccggat
     7981 ccg
//
LOCUS       DATNDT010000140         6256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4074326, whole genome shotgun sequence.
ACCESSION   DATNDT010000140 DATNDT010000000
VERSION     DATNDT010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6256
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4074326"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..531
                     /locus_tag="VN788_04435"
     CDS             <1..531
                     /locus_tag="VN788_04435"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXU19811.1"
                     /translation="GDSLDCLGGAGIDGPAALDNGGVYFSMGGLLYADPQGHVTRYGS
                     PDVEPNDMVLSHDQKTLYAGSGGKVLAFDVGADGALSNQRVFATIPGGGNDGGSIDAD
                     GRLYFTGTRPGVDVFSPSGQFLGIIPTPFPVICTAFGGPDGKTLYAVGLNAGRGGLID
                     EVLAISTIARGYLGGE"
     gene            complement(652..1098)
                     /locus_tag="VN788_04440"
     CDS             complement(652..1098)
                     /locus_tag="VN788_04440"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU19812.1"
                     /translation="MNEPIPSEVIRVGQIEIRFRLEAAQTGGHFTMFEYLIPANARVP
                     IAHSHEAFDETIYGLDGATAVTLEGRKVLVERGDVVFIPRQSVHQLENLAAVDARGLS
                     VITPGLLGPEFFREVADLLNAGGPPNVERIVAVMRRHGLRPVQPTP"
     gene            1449..2678
                     /locus_tag="VN788_04445"
     CDS             1449..2678
                     /locus_tag="VN788_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HXU19813.1"
                     /translation="MSEKYLRENQWWVSPYNFISEVTTQFQLPKRVSIHDATLRDGEQ
                     TPGVVFSVADKVAIAEKLAEIGVDRIEAGMPAVSESDAKALKEIAHLGLKSKIFAFAR
                     AMNADIDTAAECGAQGVIVEIPIGYPKLKYQFKWTWEDVLKKSVDVVNYAKKRGLYVV
                     YFPYDTTRAREEDLRNLLTRMMQDAPPDSIGIVDTMGCILPEAMKYMVRLVRSLTNLP
                     VEVHTHNDFGMAVATELAGVEAGAEVVHSCANGLGERTGNAALEELIVALHVLYGYET
                     HYDLGRLPELGALVRQLSGMQFALNKPILGERNFTRESGIGVDLVVKQPLAMFGTHPA
                     LTGRSGDVVLGKKSGKASITYHLEQMGRKDVSDDAVSEMLRRVKQLGESKRGLLNDAE
                     FKAIVDSVLSGQAVARA"
     gene            complement(2713..4506)
                     /gene="polX"
                     /locus_tag="VN788_04450"
     CDS             complement(2713..4506)
                     /gene="polX"
                     /locus_tag="VN788_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HXU19814.1"
                     /translation="MENRDVARILRETAQLLEIDGAIIGRYRSYEKVAELLYSIHERI
                     EDVAKDPKKLKELPGVGANMAEHICEILATGDYALRQKLLKKYPPTLLDLLHLQSLGP
                     KKVAFLWSNFKVATVEGVEKLAREGKLRDLPGFGEKSEQNILKAAEQHKNLSGSGRVR
                     LNAADDEAQKLSAYIATVGDIVESVTPAGSLRRGRETIGDLDLLVTMRPGRDKQKDID
                     AVAEHILKYPGIDQTLAHGENKVSFLLGSGLQVDVRLLEKKSFGAALLYFTGSKAHNV
                     ALRGRALDMGWTLNEYALTTLKGGRAVAGKTEEEVYAKLGLTYIEPELREMTAEIEAA
                     EQGKLPQLVTLKDIRGDLQMHTTASDGRNSIEEMGEAARALGYEYIALTDHSQAVTVA
                     NGMDEKRTLEQIKKIRAAQERVSSIRLLAGIEVDIKKNGTLDLDNEVLAQLDVVVASV
                     HSYMNLERAEMTDRILAAMENPYTQIIAHPTGRLLLRREPFDYDMEKILDTAAACGIA
                     MECNAYPDRLDLRDVHLRMAKHRGVKVVISTDSHATLHLRMMKYGVITARRGWLEKKD
                     VINTLPLNEFLAALRPKPESANPKTAKRKSA"
     gene            complement(4496..5098)
                     /locus_tag="VN788_04455"
     CDS             complement(4496..5098)
                     /locus_tag="VN788_04455"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXU19815.1"
                     /translation="MKSRKSRKPAKVKVLSSKTLVRARVFSVKRDRVLEPGGVTAIRE
                     FVEHPGSVVVLPVLPDGRIVLIRQYRHAAGQFLWELVAGHKEPHEDFLSGAHRELREE
                     TGYTARKIRKLLEVFPSPGLLGEKMVIFLAEGLTRGKARPEDNEKITQRVLTLTDARK
                     WIRSGRIRDSKTVAGILYYDRFIARGKIARSANRSNTSGK"
     gene            5189..5974
                     /locus_tag="VN788_04460"
     CDS             5189..5974
                     /locus_tag="VN788_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HXU19816.1"
                     /translation="MQINTVIVDDERPARDELAYLLKGFSEVNVVAQGKNGLEAVALI
                     KEHDPDLVFLDVQMPGLDGFAVIKKLMERKLRMPQIVFATAYDHYAVQAFEVNAVDYV
                     LKPFDKARIARALQRAKKMVDTNASPVERLEALVGQLGKGMPKLQPLAPVKLLVKSQS
                     RMILIDADEMIYASIQDGEITIFARDAEGVSNYRTVEELMETLDPGVFWRPHRSYLVN
                     INHIKEVMPWFKSSFMLKMNDKRASEIPVSRNQTKRLRELLKL"
     gene            6129..>6256
                     /locus_tag="VN788_04465"
     CDS             6129..>6256
                     /locus_tag="VN788_04465"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013515390.1"
                     /note="catalyzes a two-step reaction, first charging a
                     tryptophan molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HXU19817.1"
                     /translation="MAKRKRVLSGMRPTGRLHLGHYFGALQNWLKLQEEYECFFFVA"
BASE COUNT         1253 a         1752 c         1869 g         1382 t
ORIGIN      
        1 ggcgatagcc tcgactgcct cggcggtgcg gggatcgacg gtccggccgc actcgacaac
       61 ggcggggtgt atttttcgat gggcggcctg ttgtatgccg acccgcaggg ccacgtcacc
      121 cgctacggca gcccggacgt agagcccaac gacatggtcc tgagccatga tcagaagacg
      181 ctctacgcgg gcagcggcgg caaagttctc gccttcgacg tcggcgcgga cggcgcgcta
      241 agcaatcaac gcgtgtttgc aacgatcccc ggcggcggca acgacggtgg cagcattgac
      301 gcggacggac gcctttattt caccggtacc cggcccggcg tggacgtgtt ctctccaagt
      361 ggccaattcc tcggcatcat tccgactccc ttccctgtga tctgcacggc gttcggcggt
      421 ccggacggga agacgctgta cgcggtgggg ctgaacgccg gccgcggagg gctgatcgat
      481 gaggtcttgg ccatttcgac gatagcgcgg ggctacctgg gcggggagta acagttacta
      541 ggggcaatca gaagccaccg tgaactgagt ggcctgccga cgcgcagacg cggactgtcc
      601 ggactcaatt gcgatccgcg agacggaaca agtcctacgc agggacgcgg gttacggcgt
      661 tggttgaacg gggcggaggc cgtgacgtcg catgacggct acgatccgtt cgacattcgg
      721 cgggcctcct gcattgagga ggtcggccac ctcgcgaaag aactcggggc cgagcagacc
      781 tggcgtaata acgctgagtc cgcgcgcgtc gacggcagcg aggttttcga gctgatggac
      841 gctctgacgc gggataaata caacgtcgcc gcgttcgaca agcactttgc gaccctcgag
      901 agtgacggca gtggcgccgt ccaatccgta aatcgtctcg tcaaatgcct cgtggctgtg
      961 cgcaatcggc acgcgagcat tcgcggggat aagatattcg aacatcgtga aatgtcctcc
     1021 cgtttgggcc gcctccaatc taaagcggat ctcgatttga cctacgcgga tcacctcgct
     1081 gggaattggc tcgttcacgt gtacctccat ccatgtttgg cgcagaaaat atactctgga
     1141 aaggcgccag cccaaaatcg gggcggccgc tacgaacgcg agattccagc ccctgaatcc
     1201 gcgttggtgg cgagcaggta gccaagtacg tttgccgggc acggcatgct gtgccctacg
     1261 aaggcaatgg cagagatggc acagcatgct accacctagc aagcgaaagc ggcgcgagtg
     1321 gggcgacgga tgacgatacc gtttccattc gatatgagtc cgggcgaaac ggttgcggat
     1381 gtgatttcct agctcagggg gaggaaattc cgctaaactg agcctcagtt tttggagagg
     1441 ataaccagat gagcgagaaa tacctgcgtg aaaaccagtg gtgggttagc ccttataact
     1501 tcatttctga ggtaaccaca cagttccagt tgccgaagcg cgtgagcatc cacgacgcca
     1561 ccctgcgcga cggcgaacag actccgggtg tggtcttcag cgtcgcggac aaagtcgcta
     1621 tcgccgagaa gctggcggag atcggcgtgg accggattga agccggaatg cccgcagtat
     1681 ccgagtcgga cgccaaggcg ctgaaggaaa ttgctcattt gggtcttaag tcaaagattt
     1741 tcgcgttcgc gcgggccatg aatgccgata tcgacacagc ggcggagtgc ggcgcgcagg
     1801 gcgtaatcgt tgaaatcccc atcggctatc caaagctgaa gtatcagttc aagtggactt
     1861 gggaagatgt gctgaaaaaa agcgtggacg tcgtcaatta tgcgaagaag cgcggcctgt
     1921 acgtggtcta ttttccgtac gacactacgc gcgcacgcga ggaggatctg cggaatctgc
     1981 ttacgcgcat gatgcaggat gctccgccgg actcgatcgg tatcgtggat acgatgggat
     2041 gcatcttgcc ggaggccatg aagtacatgg tgcgcctggt gcgttccctg acgaaccttc
     2101 cggtggaggt gcacactcac aatgatttcg gcatggccgt cgccacggaa ctggcgggtg
     2161 tcgaggcggg cgcggaggtg gtacatagct gcgccaacgg attgggtgag cgcacgggta
     2221 acgcggcgct ggaggaatta atcgtagcct tacatgtgct ctacggatac gagactcact
     2281 atgatttggg tcgcctgccc gaattgggcg cattggtccg gcagctgagc ggaatgcaat
     2341 tcgcgctgaa caagccgatt ttgggggaac gaaatttcac gcgcgaatcg gggatcggcg
     2401 tcgatctggt cgtcaagcag ccgctggcga tgttcggaac gcatccggcg ctgacggggc
     2461 gcagcgggga cgtcgtgctg gggaagaaga gcggcaaagc gtccatcacc tatcacctgg
     2521 aacaaatggg gcggaaagac gtgagcgacg atgcggtttc ggagatgctg cggcgcgtca
     2581 agcaattggg cgagagcaag cgcgggctgc ttaacgacgc ggaattcaag gcgattgttg
     2641 acagtgtcct ctccggccaa gcggtcgcgc gagcgtaagg aactggcctt ccaccttttg
     2701 tgagacgggg tcctaagcgg atttgcgctt ggctgttttt gggttcgccg attcgggctt
     2761 tggcctcagc gctgcgagaa attcgttgag tggcagggta ttgattacat cttttttctc
     2821 gagccagccg cgccgcgccg tgattactcc gtatttcatc attcgcaggt gaagtgtggc
     2881 gtgcgaatcc gttgaaatta cgactttcac gccgcggtgc ttcgccattc gcagatgaac
     2941 atcgcgcagg tcgagccgat ccggataggc attgcactcc atggcaatgc cgcaggcggc
     3001 ggctgtatcg agaatttttt ccatgtcgta atcaaagggc tcgcggcgca gcagaaggcg
     3061 gcccgtggga tgcgcgataa tttgcgtata gggattctcc atcgccgcca aaatcctgtc
     3121 cgtcatttcg gcgcgttcca gattcatgta ggagtgaacg gacgcgacga ccacgtcgag
     3181 ctgcgcgagg acttcgttat ccagatcgag tgtgccattt ttcttgatgt ccacttcgat
     3241 gcccgcaagc agtcgaatgc tggaaacacg ttcctgcgcg gcgcggattt tcttgatctg
     3301 ctcgagcgtg cgcttttcat ccatgccgtt ggcgacagtt acggcctgcg aatgatcggt
     3361 tagcgcgatg tattcgtagc cgagagcgcg cgctgcctcg cccatttcct cgattgagtt
     3421 tcgtccatcg gaggcggtgg tgtgcatctg caaatcgccg cgaatatctt tcaatgtaac
     3481 aagctgcggc agcttgccct gttccgccgc ttcgatctcg gccgtcattt cgcgcaattc
     3541 cggctcgatg taagtaagcc cgagcttggc gtaaacttcc tcctcggtct tgcccgcgac
     3601 cgctctgccg ccttttagag tggtgagcgc gtattcgttg agcgtccagc ccatatccag
     3661 ggcgcggcct cgcaacgcca cgttgtgcgc cttcgagcct gtgaagtaga gcagcgcagc
     3721 gccgaaagat ttcttctcga gcagcctcac atctacttgc aagccgctgc ccagcagaaa
     3781 gctgactttg ttttcgccgt gcgccagcgt ttgatcgatg cccggatatt tcaaaatatg
     3841 ctcggcgacg gcatcgatat ctttctgttt gtctcgcccg ggacgcatgg ttaccagcaa
     3901 gtcgagatcg ccaatggttt cgcggccccg tcgcagcgaa cccgccggag ttaccgattc
     3961 cactatgtcg cccactgtcg caatataagc gctcagtttc tgcgcctcgt cgtccgcagc
     4021 gtttaggcgc acgcgccccg atcccgaaag atttttgtgc tgttcggcag ctttcagaat
     4081 gttctgctcg cttttttcgc cgaagcccgg cagatcgcgc agcttgcctt cgcgcgccag
     4141 cttctccacg ccttcaactg tggccacctt gaaattgctc caaaggaaag cgaccttttt
     4201 tggcccgagc gattgcagat ggagcaaatc aagcagcgtg ggcggatatt tcttcagcag
     4261 tttctggcgc agtgcgtaat cgccggtggc gaggatctcg caaatgtgct cggccatgtt
     4321 cgcgccaacg ccgggaagtt ctttcagctt cttgggatct ttggcgacgt cctcgatgcg
     4381 ctcgtgaatg ctgtaaagca gttcggccac tttttcgtag ctgcgatagc gaccgataat
     4441 tgcgccatcg atttcaagca gctgcgccgt ctctcgcaat attctggcga cgtctctatt
     4501 ttccactggt gttgctccga ttggctgacc tagctatttt tccgcgcgcg ataaatctat
     4561 cgtagtagag gatacccgcc acggtctttg agtctcgaat cctgccgctg cgaatccatt
     4621 tccgagcgtc ggtcaatgtc aaaactcgct gcgtgatttt ttcattgtcc tcgggacgcg
     4681 ctttgcctct agtgagcccc tccgccagga atatcaccat tttctcgccg agcaaaccgg
     4741 gggaagggaa cacttcgagc agtttgcgga ttttgcgcgc ggtgtagccg gtctcttcac
     4801 gcaattcgcg gtgggcgccg cttagaaaat cctcgtgcgg ttctttgtgg ccggcgacaa
     4861 gctcccacaa aaattgcccg gcggcatggc ggtactggcg aatcagcacg atgcgtccat
     4921 caggcagaac cggcagaacc actaccgacc caggatgctc cacaaactca cgaatcgccg
     4981 tgacgccgcc gggttcgagc acgcgatcgc gtttcaccga gaaaacgcgg gcgcggacga
     5041 gagtcttgct gctcagaact tttactttcg ccggctttct acttttcctc gatttcacgc
     5101 gcgctccttg gcccgcaccg aattgcaatt ctaccctagg ctcggtggtt gcccgcgatt
     5161 tcctatatac ttccatttag gcgcatccat gcagatcaac accgtcattg tggacgatga
     5221 gcgtcccgcg cgcgatgagc ttgcctacct gctgaagggt ttttccgagg tcaatgtcgt
     5281 cgctcagggt aaaaatgggc tcgaggcagt cgcgttgatt aaggagcacg atcccgattt
     5341 ggtatttctg gacgtgcaaa tgccgggcct cgatggcttc gccgtaatca agaaactgat
     5401 ggagcgcaag ctgcggatgc ctcaaatcgt tttcgccacg gcctacgatc actacgcggt
     5461 gcaggctttc gaagtgaacg ccgtggacta cgtgctcaag ccattcgaca aggcgcgcat
     5521 cgcgcgggct ttgcagcgcg ccaagaaaat ggtggatacg aatgcgtcac ccgtcgaacg
     5581 cctggaggcg ctcgtcggac agctcggcaa gggcatgccg aagctgcagc ccctagcccc
     5641 cgtgaaactg ttggtgaagt cgcaatcgcg catgattttg atcgatgcag acgaaatgat
     5701 ctacgcgtcg attcaggatg gcgagatcac aatattcgcg cgcgatgctg agggcgtctc
     5761 gaactaccgc acggtggaag agttgatgga aacgctcgat cccggcgtgt tctggcggcc
     5821 gcaccggtcg tatcttgtga atatcaacca catcaaggaa gtgatgccgt ggttcaaatc
     5881 gagcttcatg ctcaagatga atgacaagcg cgccagcgaa attcccgtct cgcgcaacca
     5941 aaccaagcgc ctgcgcgagc tgctgaagct ttagcgttct aggacgcttt tgcgcgcggg
     6001 cggctcgttg tcaactctga actttcaact ctcaactctc aactcgtcac cgcgaatttc
     6061 tgtgctagca tcgcgtggag cgttttcttt cttacatttc aatttcccgg ctgaggtttt
     6121 cccacagagt ggcgaagcgc aaacgagttc taagcggcat gaggccgacc ggccgtctgc
     6181 accttggcca ttatttcggc gccctccaga attggctgaa gctccaagaa gaatatgagt
     6241 gctttttttt cgtcgc
//
LOCUS       DATNDT010000141        49641 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2619004, whole genome shotgun sequence.
ACCESSION   DATNDT010000141 DATNDT010000000
VERSION     DATNDT010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 49641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49641
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2619004"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="VN788_04470"
     CDS             <1..234
                     /locus_tag="VN788_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU19818.1"
                     /translation="DGWVPEQKISVAEAVRAYTLGSAFASKTEHIKGSLESGKLADIA
                     VLSNDIFKIDPREIRNAKVDMTIFDGEVIYERA"
     gene            327..2372
                     /locus_tag="VN788_04475"
     CDS             327..2372
                     /locus_tag="VN788_04475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXU19819.1"
                     /translation="MAKRLFACIAAISFFCVLDAGAQGHKIIEPADIVNLQQVSDPQI
                     SPDGRYVAFVKSGPEGGPHIWLVPADGNSPARPFILSGGADVSPRWSPDSKEIAFLSN
                     RKNPLAGTGDFHFSVTGTNYKGKMPDQEEQPLAPNEGNPMPSKQIWLISLDGGEATPL
                     TNVPGGIRSFEWSKDGKSLAFIHKDPFTAEELKEKQEKKDQVLVNKNYKFDRLWIYDF
                     QTRQASLLTHDNMEIDAFAWSPDGTQFVARVSPTTSYNDHWYVSSIVLLSAKTGAVEQ
                     TLLEHSSWTAVHWSPDGRSVLFGKRGPRGIATIPVLYNLQTRNEIPIGPSYPATIGEV
                     AWTGNNSLTAAAIADTSCGFLKINLENGDLTKPSDFTGPCEKFTSSENGVLAYLRQTP
                     EHPDDVYVHSQSGERRLTRANPQVAGWALPTSQQIAWKSTKDGQTIHGILLLPPGYQK
                     GQRYKTIVQVHGGPEEAWVNGFHGEWYDWFAVLASHGYVVLLPDPRGSDGQGAAFTEA
                     NYRDWGYGDFQDIMDGVDWLVTKGIADPGRLGIGGWSYGGFMTGWTITHTDRFKVAVD
                     GAGIADVFSMAATSDIAPSYFSEYFGPFAENRSLYDAHSPVRFLEHCHTPVLVLAGEA
                     DFRVPFSQSEELYNGLHDLGREAEMVAYPREHHIFGEKAHQIDSLQRVLDWYASHLH"
     gene            2464..2604
                     /locus_tag="VN788_04480"
     CDS             2464..2604
                     /locus_tag="VN788_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19820.1"
                     /translation="MRQNLGAKRANNNAQLCLGKIGYIEILRLHGFDMARVQQALAIY
                     GL"
     gene            3213..6728
                     /locus_tag="VN788_04485"
     CDS             3213..6728
                     /locus_tag="VN788_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU19821.1"
                     /translation="MKTNLRKAGWRLLLMMVAVSAGLAIPAHGQTSPSTGAIQGLVQD
                     PSGAVVSGAAIALVNPTLGINRSVHSASDGTFLFPLLQPGSGYEITVEHSGFQKASVS
                     NVAVNVTQITAVNVRLVVGSVTQQVVVSGAATPIHTSDATLGDTLTSRVIESMPLPTR
                     DVLDILATDAGVVSQLTSPAATILQGSEAMFVGGNRATSNNYMVNGIDANNFEFHTLA
                     TGIVPIPNPDAVEEFRTETSLYDASTGFSGGGNIVLVTRRGTNKYHGTAYEFLRNTDM
                     NANDFFFNAAGKPRPVMQQNQFGFSMGGPVPKAPNTYWFFNYEGQRQRNGVTGAATGL
                     LPVLPDDRSSASLASLYNLPQTAIDPVAIAILNAPGPYGGKLFPSGSGASQGELGSFA
                     FSSPAQLNSNQYNARIDHSFRIGGMDNQFSVSYFANPDTFTNSSGANGSTIGQPYQYL
                     LQNETLSVQDTQTIRPTLLNSINVGFTYNKRDIGAIHPLTLNDFGMTRFNSSIIDRGP
                     NFSFADQMNCCGASVSVGETQHNESQDFRDMLSYSHGVHFFRFGFEARTQQFNFGSPL
                     DPGSLFFQGGLADALYGPPSDPNADLSIRDFLVGAPFFISIGSGLNDFEYRAHDFGLF
                     GEDDYHLTSRLTLNLGLRWDYLGNITEKHDLISNFDPSLLTPLAMQTGGPGLQDGFVI
                     PSGAPKFGTPGVSKSTYNNQPLKDFEPRIGFGLDVLGNGRLALRGGFGLYFSRIGPFG
                     LLQTISNPPFALSTAIVNTSQAQLLSDPFPTLPLPSQFPIFTPFPQLTSLNSDGSPNF
                     TGPQLNVIELQRDSHPPYTEQWNLTTEYQFLKGWTIQVGYMGSHGIHLQDFQGLNNAL
                     LRNANNPGPSGLDVNSAQNRESRVPYVGISSTGMLALTDAGASSYNALLVTLSHEFSR
                     GLFLKAAYTFSKTIDNVQAQAGFEPTVGPSGNQFLPQLNRGISNFDVPHRLVLTYVYD
                     IPGPRSGWQAALLNHWTLSGITTLQSGFAGEIDQFTAGNSLSGTDGYGILTPGCALVN
                     GGNVSAHTRDYLHSSCVSTTAALLSGSTFGPLSPFEGPGNQTYTIDPSNPAAIGNLQG
                     PSTRGAFFGPFETRWDMALAKTIPLRELGEQGNLQFRAEAFKTFNTPIFSNPHSVAGL
                     SSFGRITSTIDNTGRQLQLAVRLNW"
     gene            6789..9983
                     /locus_tag="VN788_04490"
     CDS             6789..9983
                     /locus_tag="VN788_04490"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HXU19822.1"
                     /translation="MTKWDNSVCIVSLALTCLLFGGASLAARTVPPNFYDSMQWRLIG
                     PFRGGRVEAVTGIPGNPLVYYFGSVAGGVWKTVDGGHTWLPLFQKELVSSIGALAIDP
                     SDPEVIYVGTGEACPRGDMSFGDGVYKSSDGGKTWSNLGLNDTRHIAKIIVDPRNPEV
                     IYVAALGSVYGPSEDRGVFRSSDGGKTWQKVLFHDASTGAIDLAMDPHNRHVLFAALW
                     QVRRKPWDFTSGGPGSGLYRSTDGGDHWTEIKGHGLPVGVLGRIGVSVGAQSNRIYAI
                     VEAKDGGLYRSDDGGTNWNRVNGSWDLRGRPWYYSHIFADPNDVDEVYVFSFGAYRSV
                     DGGKTFAALYTPHGDYHALWIDPRDSRRLIVGNDGGATISVDDGTSWTTEDNQPTGQF
                     YRVATDNRFNYYLYGSQQDRGTVAITTRSDEALIGQNDWFSVGGGESGFVLPSPLNSD
                     VVYAGSLYSAFTRFDRGAAQSRDISPWPLNELNLPAKDARYRFGWTAPMLISPHDPHT
                     LYIGAQVLFKTRDEGKTWDVISPDLTRNDRSKQNSAGGPITQDNTTVEYFDMISTIAE
                     SPLESGLIWVGTDDGWIQVTRDAGKSWVNVTPKGMPDWSLVSMIEPSRFSVGTAYAVV
                     DDHQLNDFRPHVYRTADFGRTWVSITEGLPADSYAHTVREDPARRGLLYAGTETGVFV
                     SFDDGSHWQSLQLNLPTVPVYDLTVHENDLAVATHGRGFWVLDDLTPLRQAAEASASS
                     DFYLYRPEPAYRVRGNNFYATVSSGAPSSPVAGQNPPAGAILDYYLSAQPQTSAKLEI
                     VDDKGRIVREFDLRSAAVSASGKQVLITAGAVTLADGQEYRTDPTVHRGLNRFAWDLL
                     YQGNESSPRGMRVPEPLVVPGNYVVRLTVDGKTQKQPLVVREDPRVQVSLADLQAQFN
                     LQSKIADRLQEAAVAVAEMKQVRGELVALGKRLDASRSPEKLKKALDNVESKTEDAER
                     SITGWKAAGDGYALNYPPALDDQINWLFMFAGAGDGAPGQPFLDLYDQLSRELAADLG
                     RWHEIKTADLVQLNELIRENKIPAIAAFAPPPSH"
     gene            complement(9994..11322)
                     /locus_tag="VN788_04495"
     CDS             complement(9994..11322)
                     /locus_tag="VN788_04495"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HXU19823.1"
                     /translation="MSKHWRIAAVAACAALSISWGARGRTRDAYQRAAIGAAHWIEEN
                     GSNTGNGIVWPADPRNPESVNTTLYAGTPGPILFFLEAYRYTQDRDFLEKGKKGADAL
                     LASISEKDGTGLYEGLAGSGFTLGEAYLITHDTRYRDGALLCVRWLEKRAQMTPTGAK
                     WNDVTDVIAGSSGTGLFLLWAADHLQAPGAKQLATRAGDFLIATAQRKQDGLMWMMDP
                     SYPREMPNFSHGTAGVSYFLATLYQATGQEKFLDAALAGAKYLLSIADLNNRYCMIYH
                     DSEHQDLFYLGWCHGPAGTARLFYRLYQVTRDAQWRDLVEKCAEAVVANGGPAKAVTP
                     GVWHNVSMCCGIAAESEFLYDAFLVTHDRRWFRAAKKGSEQIVELADEDGKRYRWVQV
                     ETRVRPDIAVAQTGYMQGASGIGMWLLHFSDALAGGHRPVLTFPDNPFAY"
     gene            complement(11375..12772)
                     /locus_tag="VN788_04500"
     CDS             complement(11375..12772)
                     /locus_tag="VN788_04500"
                     /inference="COORDINATES: protein motif:HMM:NF017000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HXU19824.1"
                     /translation="MKHLLQGVAVVLIVLMVSILPAQAADSPYEKAALGAAQWLRMNG
                     TAKNSGIVWAANPEDPKSMNTTLFAGTPGPILFFLEAYRYTGNRVYLEQARKGADALL
                     ASVSKNDDTGLYTGLAGSGFTLGEAYLITRDAKYREGALQCLHWIEAKATNTADGSQW
                     NGVTDIISGASGTGLFLLWAADHLQAVEARALAVRVGEHLIRMGQPQPGGGLKWMMDN
                     SYPFEMPNFCHGTAGVAYFLATLYQVTGRKEFLEAARKGAKHLIAIANSSEQYFLVYH
                     DNHNTHLFYLDWAHGPAGTAALFYRLYQITKQPKWLELVEKCAAATRIFGGPEKAFTT
                     IPSGSATTLTVWRQGQPKQVPNPGVWRNVSICDGTAGEAEFFYNVYLVAHDRKWLQAA
                     KEGSEDLLRRADVHDGQYRWVQVETFVRPDYAIAQTSYMQGASGIGMWLLHFSAALAG
                     QHRPVIRFPDNPFPY"
     gene            complement(12796..14961)
                     /locus_tag="VN788_04505"
     CDS             complement(12796..14961)
                     /locus_tag="VN788_04505"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU19825.1"
                     /translation="MRSKFRSSRLSSFVGSDNCGSLRLRSLPACLSGFLGIVLSVVSA
                     VFFAPPLWAEAGSVVIDHGARDPAMSPDGTRIAVSVLGRICLLPAAGGTGQLISSGIG
                     WDRFPAWSPDGAFLAFSHEVNYRSYLVVYDIPVGTWAVVYETDGVIGQIAYDPAGGDI
                     FFTVRHGQGDAHIWKIPTSGGAPEEITETRGWHEWSFAPSPSGKRLLLTSGLYGGANL
                     YMMDVEKRRAVRLTNTPFDDTSVAWSRDGVRVAYVETGNGADEIRVDQAEGNGKPRVA
                     FRSAYQDEQITFGPDANTLIVCAARKLYRLDISSGALAPIPFHVAFQVPPQWPADLLV
                     IHAHLIDGTGRPPIADASVLIRGGRIVKIQRGAFRPGDIAGVPVFDAQGDSVLPGLMD
                     NHYHFWDAFDGSRLLSRGITCIRDPGADLADSLNFKEAIALGLEPGPDIYTAGPLIDG
                     FGTYHPMVAVQINQAEQAKPLVDSLKAQGVDLLKVYFLLKPEVLRAVIEAAHRDHLRV
                     TGHIGVRTSWTEAIGAGIDGLNHIRVWPEFLPASAQPQGADRTLDATWHLVARMQADW
                     RNIDPNGARAQELIARMLRAKIGFDPTLSIQSPAPSLRKQLSLEEVATWEQSYERMSQ
                     FVHNAVAAGVPLLAGTDDGSLFDELEAYEQAGIPREQIIRAATGSGASWLGRGDDFGT
                     IQVGRRADIIVVHGDPLASIKSLRNIIVVVKNGRLVFHK"
     gene            complement(14965..15381)
                     /locus_tag="VN788_04510"
     CDS             complement(14965..15381)
                     /locus_tag="VN788_04510"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXU19826.1"
                     /translation="MAEINATVHYGGNGFFVGISPGGQALVMETNSERSSAPTPVELL
                     LLAIGGCMGSDVVDILTKKREQVTDYRIEVHGERRQEAPRIYETIRVHHVVKGPNLSE
                     DAVRHAVELSNAKYCSVAASLRPTAEVSVTIQVVRG"
     gene            15544..16560
                     /locus_tag="VN788_04515"
     CDS             15544..16560
                     /locus_tag="VN788_04515"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXU19827.1"
                     /translation="MELRQIEIFKMLAEELNFTRAGRRIHCVQSNVSVQIRSLEKEFG
                     VQLFERLGQRVRLTAQGQTFLPYAERILRLIQEAAAVTRGGENPAGTLVIGMPESVLT
                     YRLPPVLQVFRSNWPHVELVFRGISSLELIPCLERGDLDLGLVIDDAIRIPGIHAEVL
                     CNEPLVLTVQPGHPLLRAPIVQPEDLGSYTFLLTDQGCAYRSKLETALARVHVRPRTV
                     MEFTSVEAIKQCAALGMGIACLPKIVVERELASSKLVVLPAASFELTMNTLAVWHRDK
                     WISPAMKAFLSLIRLHLGSASISNVPTKSPATIIDGRKPAASAAGKNREARMTSGRHR
                     GVAP"
     gene            complement(16575..16757)
                     /locus_tag="VN788_04520"
     CDS             complement(16575..16757)
                     /locus_tag="VN788_04520"
                     /inference="COORDINATES: protein motif:HMM:NF022018.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HXU19828.1"
                     /translation="MPKLTSQERAMTKKEAMVRAERIESRILLIRGQRVMLDSDLAEL
                     YGASRRMAARLPGCPN"
     gene            16886..17575
                     /locus_tag="VN788_04525"
     CDS             16886..17575
                     /locus_tag="VN788_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015177776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HXU19829.1"
                     /translation="MCNRYRLTHAQRYLAERFQAWDDFDETPRYNIAPTQPVLTVRRE
                     QGKKIRRFTMMRWGLIPHWAKGTSIGTRTLNARSETVTSTPPFRESILTKRCLIPADG
                     FYEWRAMGRIKQPFCFEVGEGDVFAFAGLWGQWEGPEGEVIESCTILTTGPNSLVAEM
                     HDRMPVIVPPDKYEIWLDPDITNFDAIKDILKPYDAGQMRRYPVSTKLNNSKNEGAES
                     AAPVTLDVPLF"
     gene            17634..18116
                     /locus_tag="VN788_04530"
     CDS             17634..18116
                     /locus_tag="VN788_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008533483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU19830.1"
                     /translation="MTQQIVDVHINPSEEAIRLGPVAVRFLITGENSSGSIAAFEVIV
                     PGAQRLTAPAHSHDHYEETIYGIAGILTWTVDGKQIDVAPGQALCIPRGAVHRFDNNA
                     SQHAKALCVITPAAIGPQFFRECADVLRATAGGPPDRAKLVEIMRRHGLTPAPPPANA
                     "
     gene            18322..18558
                     /locus_tag="VN788_04535"
     CDS             18322..18558
                     /locus_tag="VN788_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19831.1"
                     /translation="MPDEEKGAAKNGGCELLLALIHDATIEGLDGTHVVRVELCKGGN
                     FAGCDLRKRFEHGLCQRTGCMARGYRIADNHMDI"
     gene            complement(18714..21113)
                     /locus_tag="VN788_04540"
     CDS             complement(18714..21113)
                     /locus_tag="VN788_04540"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HXU19832.1"
                     /translation="MGNAKRRAALFFVTVFFSVGPLWQFAIRGLTQSSTAKLDSRAKK
                     AISEIRGTLQIAGLEKPVTVLRDRWGVAHIYAQNQHDLFFAQGYVVSQDRLFQMELWK
                     RSGEGRLAEILGPSALQRDVNARLLRYSGDIDAEYKSYSPDTKEILRAFTDGINAYIA
                     ERTRPSGPGLPIEFQIAGFKPEPWRPEDCLNRMAAFSMTGNSTAELYHAEAVARIGAA
                     RATKLFALDPAVQLDPAPGVNFAGLSPQLLRNLVGSDTRIEFPASALEGSNDWTVSGA
                     LTASGKPIVANDPHRVIAEPSLRYIVHLVAPGWDVIGAGEPGLPGVAVGHNQNIAWGF
                     TIFGLDQQDLYIEKLNAANPLEYKTERGWAKMRVEKETFGVRGQTNVVVDLKFTRHGP
                     VLWADKTRALALRWVGAEPGTAGYLGSLAVDRAQNWQQFVEAMKRWKVPSENIVYADR
                     AENIGEYSVGLAPVRKNWYGLLPVPGDGGYEWNGFLPLAELPHSFNPERGFVATANHK
                     MIPPNYPYRVGYEWAPGFRFRRVTAVLEAAKSSGHKLTVADMEKLQNDVLSTPAVELI
                     GLLRQAAGNSSDPDVRLLLGWNGLVARDSAAAALYELWLPEVTAAVSRRAVPKELWNM
                     MRGWPVAKVLDSISHPSPEIFGGHPEAARNELLLDALKSAHEKLAQLEGPDPAKWSWG
                     RIHVVEFRHPLDEAPQGKILFDLGPIARPGDDYTVDATGFRGSDFRQVAGASYREIMD
                     LSDWDKSVAVNVPGQSGQPEAPHYSDLLPLWGDGRYFPLWFSRGAVQAHAADKLVLKP
                     K"
     gene            complement(21425..21964)
                     /locus_tag="VN788_04545"
     CDS             complement(21425..21964)
                     /locus_tag="VN788_04545"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HXU19833.1"
                     /translation="MSPELVSPWREFLEDLDNLLPARLDLHCIGGFAAVAMYGLPRST
                     NGLDYIHIESNNAPDLESIAGEGSPLAKKHKVFVHYVTIATYPEDYDRRLKQLFPNRF
                     KNLRLLVLDPYDLVLSKLSRNSAKDQEDVKYLARMQHLDPEILRERYNEELRCNLIGP
                     AERADNTLKFWIEAYFSQQ"
     gene            complement(21954..22634)
                     /locus_tag="VN788_04550"
     CDS             complement(21954..22634)
                     /locus_tag="VN788_04550"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU19834.1"
                     /translation="MSYNIHMTSEQLRQARFKQGQTQQQVAARLGVTQAYYSMLESGA
                     RKPSAELARKLIREYHVSPVVLPVSETTMLNPTPAFLARELAALDYPGFAHLRRGSKK
                     VNPAQFLLLALKQNNLEARVAEGLPWLVFRYPDMDFRWLTAQARMNNLQNRLGFTVTL
                     AKIAGHNADLEGPEQVLTESKLAKEDTFCRELNEPERRWLRENRSEEARQWNLLSDLR
                     SDQLRYVA"
     gene            22707..23033
                     /locus_tag="VN788_04555"
     CDS             22707..23033
                     /locus_tag="VN788_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017296448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXU19835.1"
                     /translation="MEVREYLDRRGHSPFAAWSDSLNKEAAAKVAAALARMQQGNFSN
                     AKGVGTGVYEYRIDFGPGYRIYFGKDGERLVILLGGGTKKRQQTDISTALARWQDYKQ
                     RKGTER"
     gene            23039..23344
                     /locus_tag="VN788_04560"
                     /pseudo
     CDS             23039..23344
                     /locus_tag="VN788_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726658.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            complement(23419..23700)
                     /locus_tag="VN788_04565"
     CDS             complement(23419..23700)
                     /locus_tag="VN788_04565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19836.1"
                     /translation="MMQYLVQLRLSSSARPMSPGDGFIEELILPTLERCDKLQKDRKM
                     LAGGPMSGAVALALIVTAESAQELDHLITSLPVWQRMEIRSSDSPATAG"
     gene            complement(24100..24885)
                     /locus_tag="VN788_04570"
     CDS             complement(24100..24885)
                     /locus_tag="VN788_04570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008061623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19837.1"
                     /translation="MKNLHGKWALVTGASSGLGVEFAKLFAERNANLVLVARRTEPME
                     QLAEALRKEQSVQVVVMGMDLSQPGAAAELKAQLDARGIEVEVLVNNAGFGAYGDFLD
                     QSVEKLGEMMRLNVMALTELTHIFGWEMANRGHGRVLLVASVLGYQAVPGYAAYAATK
                     GYVLLLGEALHQELSPRGVSVTTVSPGATATAFSAVAGEKSSLVLKALRMKPQVVAKT
                     AVLAAMRDKASVVPGFLNKANVFMDRLMPRSMQRMVFGRVMAG"
     gene            complement(24950..26296)
                     /locus_tag="VN788_04575"
     CDS             complement(24950..26296)
                     /locus_tag="VN788_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013820956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HXU19838.1"
                     /translation="MQLAEHPTVKQFQARETANKEVVRPQTLDREWLRQMCLEAGADD
                     VGFVEIDRPEIADQKTDVLWLLPKSKTLVSFVLRMNRENIRTPARSVANLEFHHTTDE
                     TNEVARKIVAALERIGVRAVNGGAAGFPMEADRWGAKMWVVSHKPVAVAAGLGRMGIH
                     RNVIHPKFGNFILLGTVLMDAEASEYSHPIDYNPCLECKLCVAACPTGAIGSDGSFNF
                     SACYTHNYRDFMGGFNDWVEKIADSKDALDYRRKVSGAESVSMWQSLSFGANYKAAYC
                     LSVCPAGEEVIAPFLTNRKQFLADVVKPLQDKVETVYVVPGSDAEDYVARRFPHKKTK
                     RVSNGLAGQGTIRAFLRGLNFVFQSGKSEGLNATYHFTFTGQEEVKATVVIRDKKLAV
                     SDGHSGTPDFRITADSQTWLRFLRKEANLAWALLTRKFRIHGSPKVLLAFARCFPS"
     gene            complement(26349..26810)
                     /locus_tag="VN788_04580"
     CDS             complement(26349..26810)
                     /locus_tag="VN788_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HXU19839.1"
                     /translation="MSTQNTSAEYIAGLPTLPCTCANLRRAARAVTRLYNHELRPQQI
                     EITQFTILMALARAGEITQGKLGKVLALDSTTLTRMLELLRNRGWIQEKEGEDRRFRI
                     IRLTNAGRAKLQHSVPHWKRAQNRLQNVLGEGTMGQLAVLLAKVTATSAGG"
     gene            complement(27151..30357)
                     /locus_tag="VN788_04585"
     CDS             complement(27151..30357)
                     /locus_tag="VN788_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXU19840.1"
                     /translation="MIARVIHWCTRNAFLVFTAVALLVLGGLWAMRRIPLDALPDISD
                     VEVIIYTNWAGEPPNITEDQVTYPIVTTMLAAPQVKAVRAQTMFGDSYVFVVFEDGTD
                     LYWARSRVLEYLQQIQGRLPAAVHPVIGPDATGAGWVYEYALVDHTHKYSLADLRSLQ
                     DWYLRYQLETVPGVAEVASIGGFVRQYQVNLDPDRLRAYNIPLSTVIDRVRASTNEVG
                     GRLLELGGAEYMIRGRGYLHSLNDLEDVPVATKNGTPVLIRDLGTVSFGPDIRRGVAE
                     WNGEGQTVGGIIVMRYGMNALTVINGVKQKLAQIKKSLPPGIEVVSGYDRSGLIQASI
                     HTLERDLIEEAIVVSLVIIIFLWHFRSALIPILTIPIAVVASFIPMYYLHVSSNIMSL
                     GGLALAIGVLVDAAIVMVENGYRHLAERQSHPPDQKNLPFDSAVASREASPSAVRIPQ
                     TERRSILLAAAQQVGPALFFSLIIIVVSFLPVFLLEAQEGRMFKPLAWTKTLAIAFSS
                     VLAITLVPVLMPLFVRGRLRAEARNPVSRITQAIYLPFLRLCLRFRKSTIIVALICLA
                     LSVPLARRIGSQFMPPLFEGSALYMPTALPGISIGQASDLLQEQDRIIKTFPEVESVF
                     GTVGRSDSATDNAPLDMYDTTIMLKPRNQWPAGMTYEKLIQQMDAKLQFPGLSNTWTM
                     PVENRLDMELTGIKTPLGMKIQGPSLDGIQQVGAKIQQLLSGLPETRSIFAERVSQGF
                     YINVAMNREVAARYGLTVADVQRAVESGIGGEDIAENIEGRERYPINVRYNRDFRGNL
                     DELRQVLIATPSGAQIPISEIANVSFSRGPAMIRDEDGALTGYIYIDLNTSDYGGFVQ
                     KADSLLRQKLALPAGYTYQWSGEYQFELRAKKRLELILPVVFFVIFLLLYMTFHSVAE
                     SAMLFFPVFFALIGGLLLQVILGYNFSVAVAVGYIDLFGIAVETAVVMIVYLHEALDA
                     RLALGIPLKHEDIEAATIDGAVLRLRPKLMTVFAVIGSLGPLLWETGIGSDVMKPIAA
                     PIVGGMVTSTIAVMILVPILFAQLKERALRHGTLRSTQ"
     gene            complement(30359..31921)
                     /locus_tag="VN788_04590"
     CDS             complement(30359..31921)
                     /locus_tag="VN788_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU19841.1"
                     /translation="MKTYRAGFIWALAANVVFIAVIAFVWFRYHPGDPTPNAQSPKSS
                     VMVQRTPAPNPAPSTTAPETRLVPVQISAQRLQSIGVTLGQVQRKYVEDEIRTTGNVA
                     IDETKLAYVQVRFSGYIQRVFADATYQYVHKGQPLFTIYSPDLVATEREYILAKQNQQ
                     QVAHSTVPGVASGAASLVSAALERLTQWGIPRREIARLESTDQVQQELEIDSPISGYI
                     TERNALPGLTVQPETRLYTIADLSTVWVQVQIFQNDLGRIRKGDPASLAVDSYPGRTF
                     SGHLDLIYPQVDMNTRTANVRIVFANPELLLKPGMFVSVSLKIPMGRQLVIPASGVLQ
                     SGMRSIAFVDRGDGYLDPRDVQLGARVGDNFIVLKGLQAGERIVTSANFLIDSESQLQ
                     AALGSFIPPPPGAGAASAINVSQSNIEFSSEPNPPHKGTNIFRVKLTNRSRAPILGAQ
                     VRVTFFMPAMPGMGMSAMRTTATLTDKGSGVYEGTGQLGSGGTWQVTIVAQKNGQTIG
                     SKQLSVNAAGGM"
     gene            complement(31918..33282)
                     /locus_tag="VN788_04595"
     CDS             complement(31918..33282)
                     /locus_tag="VN788_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXU19842.1"
                     /translation="MFPPFRQLRTRKLTFAPCLIAALLLFLLQASEVFGQTPVTPNNP
                     VHVVAADAQTPARLSDLLTEAQRNNPQIQAAHQAWIAAQQVPAEVSALPDPQVQVQQF
                     SVGSPRPFAGYTNSNFAYIGLGASQDFPYPGKLRLKGQIAKQDSAVAQQQYESVRREV
                     LANVKSAYFRLAYLSQTLAILQSDGELLNEAAQAADAQYRAGMGNQQDLLQAQLEKTK
                     LLREIIMHHLEVAKLEAQMKQLLNRSQSSPDIETIQISQTPLTYTFDELLNAAKSQNP
                     EITAAQSLVQKGKLQVDLAHKDFYPDFNLQYMWQRTDPTQYRAYYMLTFGVNIPIHRS
                     KLHHELAEAQADLNRSRSEAEAQFQQVAFQLRTEYDIAQKDAELLNMYNHGLLPQARA
                     EYQAGIASYQNNRSDFQTLLNSFLDVLHLDEECWQTFADREDALAGIEQLTGLSLRQE
                     GAQQ"
     gene            35541..35864
                     /locus_tag="VN788_04600"
     CDS             35541..35864
                     /locus_tag="VN788_04600"
                     /inference="COORDINATES: protein motif:HMM:NF022321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2703 domain-containing protein"
                     /protein_id="HXU19843.1"
                     /translation="MEIEVLYVSECPAHPAALKLVKDVLAAQGVAADIHEVLVADEKM
                     AHELQFVGSPTIRINGRDVAEESQERQNFSLSCRLYAGSKYVGLPPVEVIHRAVSEAR
                     RGGKP"
     gene            35861..36199
                     /locus_tag="VN788_04605"
     CDS             35861..36199
                     /locus_tag="VN788_04605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19844.1"
                     /translation="MKQAKGISASIGAILSVLATVGCCFPIGFTAALGAGAASAFFTT
                     LRPFLLGLSIVLIGLGFWQQRRAKQCALRGRWISEIMLWAAVVIVVGMILFPQQVAGF
                     LAGEFWRWPK"
     gene            36196..36396
                     /locus_tag="VN788_04610"
     CDS             36196..36396
                     /locus_tag="VN788_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19845.1"
                     /translation="MKKRLVVLGLVVLAAVVAAIFLGGPGTVPAGQKPLATLSSANLG
                     AFERAFNGNADMPRLVLLLSPT"
     gene            complement(37203..37772)
                     /gene="greB"
                     /locus_tag="VN788_04615"
     CDS             complement(37203..37772)
                     /gene="greB"
                     /locus_tag="VN788_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HXU19846.1"
                     /translation="MREPPGPALKNYITPAGLQRLKDEHVFLLSRERPAVVEVVAWAA
                     SNGDRSENADYLYGKRRLGQIDSRIRFLTKRIDAAVVVDPAARRPDSVATRVFFGATV
                     TYKDASGLEHVVSIVGIDEVDLSRGYISWRSPLAIALMKASPGDHVELRAPAKTEHLE
                     IIDVEYAPIPIDPFREPLGAQSTPKIERS"
     gene            38386..38577
                     /locus_tag="VN788_04620"
     CDS             38386..38577
                     /locus_tag="VN788_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19847.1"
                     /translation="MKCLVAIAEYNSETKGTAFSIELGELYYNRMLRFVGIKQCEAQL
                     SKVLDSNNAANDQAVLVSP"
     gene            38606..39241
                     /locus_tag="VN788_04625"
     CDS             38606..39241
                     /locus_tag="VN788_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19848.1"
                     /translation="MSALVFRNWKARAYFSSSDSDLASVRATSLDPVRIISSFCYYFE
                     RGSSPLPSQWGLFPVSSKFVTPSAILPRMNRARVTVALCLLLFFPPITSRTSGAQGQV
                     SMTLEQVHSIVQKYAEQWNRQNPKYPLWVALASPGDPVNIPGATFGTSDNPQAGMELP
                     VCAHVIVINKSMVPESILTTFTHEYGHLNIDWPTPVILTMLLQKLPPLRAR"
     gene            39545..40201
                     /locus_tag="VN788_04630"
     CDS             39545..40201
                     /locus_tag="VN788_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19849.1"
                     /translation="MKTNVFSKPEESRDRLRQLGLDALVFVQAIQRGQAIAAGYTPNH
                     PAICRGISQWGEVVAAIREQLLPRAWTRMDEDNFPLTVNPPRNIAISVSTGDASTGIV
                     DATPSTRSTKGPRTANAVIVNALQLNLFGNVPLRPEELKCSNGVMTWIFLFHRDVAAQ
                     ETRFELSRPVKMDSDGHVDVWVERIIFPALPFGTDGLLVPADLPVSPEIIPEVKRKRA
                     "
     gene            40194..41297
                     /locus_tag="VN788_04635"
     CDS             40194..41297
                     /locus_tag="VN788_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018293196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HXU19850.1"
                     /translation="MPEAAGMFNPNRLSVARKRQGLTKTDLAKRARVDLRSVTAYEAG
                     VYPPKLETAESLAAATKFPLEFLYGDDLDEPQPDTASFRAMSKMTATQRDMALSQGTI
                     ALHLSRWLETRFELPELDLPDLSREDSPEAAADSLRRHWGIGELPVRNMIHLLEAKGV
                     RIFSLAINAREVDAFSLWKDITPFVFLNGNKSSEHSRYDAAHELGHLVLHRHGTPQGR
                     DSEREADRFASTFLMPTSSIIAHAMRNPSLPDLIKLKVIWIVSVAALNRRLYSAGMIS
                     DWQYRTLCIEIAKRGYRKHEPREAPREMSHILPSVFGALYEDGIGRADVAKALKIPRS
                     ELEQLMFGLTMTSIDGGRNQTGSPVKAKLTVVE"
     gene            complement(41669..42505)
                     /locus_tag="VN788_04640"
     CDS             complement(41669..42505)
                     /locus_tag="VN788_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19851.1"
                     /translation="MNASLRALISEAMSKRMANPEARARLSESTKKQWANPITRAKWL
                     AAFKHRKPEARAAFLAANKKAWADPQKRAERIAAMRKAASDPQRRARLAAKAKARWST
                     PEAKARLSEFSKKQWANPLTGEKMIAAIKEAAARPEVKAAVAAAIKKGWAPEKRAARI
                     ASMKKNLSDPEINARRVATLRKTLASPEERARRSARMKDVWRKLKGGRPADERKWQLA
                     ITLRAEGHTWTRVARKIAPKAFAVDPRKAGRAVRSGVERYMKKGSTEHRDGSERGREA
                     CT"
     gene            43112..44281
                     /locus_tag="VN788_04645"
     CDS             43112..44281
                     /locus_tag="VN788_04645"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXU19852.1"
                     /translation="MARRRFQTGWIRVRGKNRPVFEGRWREDVIQPDGTIRRVPRSMV
                     LGTVSELKTKRNAQRLLEPILARINSFDYRPSKFTTIERFADTWETQVLIHQKPSSVK
                     AAKSHLRSHIRKQLGKVLLHEFTTQMQQAFVTLLAQKRVSRKTIQNVLGTLASMVKTA
                     KAWGYSIQPIVTGELHLPADSVREPARFFTGEEAKNIITIAPQPYRAMFAIAGMAGLR
                     VGEIVGLQKADLDFDTRRIFVRRSAWYGRVQTAKSPASQKPVAMSKDLVPLLEEYLET
                     WRPNPEGFLFLNRNGRPYAANKVVEYGLWPVLDKLKIPRAGMHAFRHAHNSLLGHVGA
                     NPIVMKTQMRHSDARVTLDFYSHIIGDDQRAAVDKVGEILRPNEPKSGSDGEYIQ"
     gene            complement(44505..47747)
                     /locus_tag="VN788_04650"
     CDS             complement(44505..47747)
                     /locus_tag="VN788_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011940111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXU19853.1"
                     /translation="MNLSKIFIHRRVMTSLVMLAILGFGILAYRDLAVSDLPNIDFPT
                     IQVSAGLPGASPDTMASSVATPLEKQFTTIAGLDSMTSTSSLGRSQITLQFSLSRDIN
                     SAAVDVQTAISAAANQLPANMPFPPTFHKVNPADQSIFSLAVTSPTLPLYEVDKYAEN
                     LIATQISMIPGVAQVNVNGQATYAVRVGLDPDAMASRQIGIDEVEQAIQSANVNMPMG
                     TLFGAHKAYNLQSNGQLNDAAAYRPLIVAYRNGAPVRLDQIASVTDGVQDRYVANWIN
                     SIPGILLGVQRQPGTNTIDIVNRIRKLIPHFFTLIPPSINLSIEYDRSVPIRQSVNDV
                     KFTLLLAVILVVLVIFLFLRNASATVIPSLALPMAIVGTFAVMYLLGYTIDNLSLLAL
                     TLSVGFVVDDAIVMLENIVRHMEMGKDPVEASLDGSQEVGFTILSMTLSLAAVFLPVF
                     FMAGVFGRLLHEFSVVIISAVLVSGFVSLTLTPMVCSRYLRAEHEKQHGWLYRKLEGV
                     LDGSLRWYGVSLRWSLRHRVFIMLVGAAILVGTIWEFWIIPKGFLPEEDLSQIQVSTE
                     ANQGISFDAMVAHQEAINRIFQADPNVVQFHSSVSDSSSNGLNNGNIFLHLRDLPDRP
                     WTDSPAYDRLVTSYGHTPVLDSLVRLIRPLFEHHMTIDDIMQELRPKLKRVPGIEVFL
                     QNPPAIRIGGRQSKSLYQFTLSSPETGTLYDHAQELVQKLKDLPDLVGVTSDLQAKNP
                     QANVVVDRDKASALGVTPQQVEGALYSAYGQRLVSPIYTSNNEYWVVLDLQQRFQADP
                     NMLSDLYIHSTSGQLVPLSAVSKFTTNVGPLTVNHTGQLPSVTISFSLAPGVSLGQAV
                     AEVQKLASSMLPISISTSFQGSAQAFQESLTSLPILLIMAVLVIYLVLGILYESYVHP
                     VTILSGLPAAAFGGLLTLSLFRMQLDIYGFVGLIMLIGIVKKNAIMMVDFALDLERQG
                     EISPAESVYQGSMIRFRPIMMTTAAAIMGTLPIAIGFGADAGARRPLGLCVVGGLIFA
                     QFVTLYYTPVFYTYADNFLKWRKKAKVAPAREIAGLQEPLLDPRLKRLGR"
     gene            complement(47762..48901)
                     /locus_tag="VN788_04655"
     CDS             complement(47762..48901)
                     /locus_tag="VN788_04655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU19854.1"
                     /translation="MIGVLKYRAMILLLFGLCLVGCTDSPGTKQGSDASGAITRPQST
                     SAVPVVTAKVERKSIPIELHAIGTGQAFKTVSVASQTAGIVKEVNYRPGQFVREGDLL
                     VSLDKAPFLAALSQAQAALARDKAQAQLGRADMQRYDQLYKEGVVSKQQYDQSEATSA
                     SAGATVAADEAAVETAKIQLSYCSIKAPISGVTGAQLVYPGATVAANGTPPLVVINQV
                     SPLYVTFSVPQQYLSPIKRAMERSRLAAQATPPDSTVPEKGFLSFVNNTVDANTGTIQ
                     LMGTFANTDHHLWPGQYSNVLLRLGEQENVMVVPSQAVQSGQQGQFVFIVKPDMTVDV
                     RQVTAGQTVENQAEILKGLAVGETVVTDGQGSLVPGTKVYFTKAI"
     gene            complement(49440..>49641)
                     /locus_tag="VN788_04660"
     CDS             complement(49440..>49641)
                     /locus_tag="VN788_04660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19855.1"
                     /translation="EGKMATATKAIAPKNGERVRSAEHEGVFEIVGVNSLMQTANIRL
                     IDGSGHVVPNVPWTALLPANKK"
BASE COUNT        10911 a        13893 c        13987 g        10850 t
ORIGIN      
        1 gatggctggg tgccggaaca gaaaatctct gttgcggaag cggttcgcgc atacacgctg
       61 ggttcggcgt tcgcgtcaaa aaccgaacac atcaaaggct ctctcgaatc cggcaagctg
      121 gccgatatcg ccgttttatc caacgacatt ttcaagatcg atccgcgcga aattcgcaac
      181 gcaaaagtcg acatgacgat tttcgatggt gaagtcattt acgaacgcgc ctagttcgcg
      241 ggaaacacta gccgcgccct gaatgctgcg gcatctgcga gattcgaaac gaaatcttgg
      301 tcatcttgct gtctcttgga ggagccatgg cgaagcgtct gtttgcctgt atcgcagcca
      361 tttcattctt ttgtgttctc gatgccggcg cgcaaggaca caagattatc gaaccggccg
      421 acattgtgaa cctgcagcag gtgagcgatc cccagatttc tccagacggc cgctacgtcg
      481 catttgtcaa atccggcccg gagggcggtc cgcacatttg gctcgttcct gctgacggaa
      541 acagtcccgc gcggccgttc attctgagcg gcggcgcaga tgtttcgccg cgttggtcgc
      601 cagatagcaa agaaattgcg tttctctcaa accgcaagaa tccgctcgcg ggcactggtg
      661 atttccattt ctctgttacc ggaacgaatt acaaaggcaa gatgcccgac caggaagagc
      721 agcccctggc gcccaatgaa ggaaatccca tgccgagcaa gcagatctgg ctgatttcgc
      781 ttgacggcgg agaagcgaca cctttgacca atgtccccgg cggtatccgc agcttcgagt
      841 ggtctaagga tggaaagtct cttgccttta tccacaaaga tccattcacg gccgaggagc
      901 tgaaggaaaa gcaggaaaag aaagatcaag ttctggtgaa caagaattac aaattcgatc
      961 gcctgtggat ctacgacttc caaacgcggc aagcgagctt gctcacgcac gacaatatgg
     1021 aaattgacgc cttcgcttgg tcgcccgacg gaacgcaatt cgtggcgcgc gtttctccca
     1081 cgacgagcta caacgaccac tggtacgtct cgagcattgt gctgctgagc gcgaaaacag
     1141 gcgcggtgga acaaacgctg ctcgaacatt cctcgtggac tgccgttcat tggtcgcccg
     1201 atggccggag cgtcctcttc ggaaagcgcg ggccgcgcgg aattgccacg attcccgttc
     1261 tttacaattt gcaaacgcga aacgagattc ccatcgggcc ttcgtatccg gcgacaatcg
     1321 gcgaagtcgc gtggacgggc aataactcgc taaccgcggc agctattgcg gatacatcct
     1381 gcggcttcct caaaatcaat ctggaaaatg gcgacctaac gaagcccagc gatttcaccg
     1441 gtccatgcga gaaattcact tccagcgaga atggggtcct cgcctatctg cgtcagacgc
     1501 cagagcatcc tgacgacgtt tacgttcatt cgcaaagcgg cgaacggcgg ctcactagag
     1561 cgaatccgca agtggccggc tgggcccttc cgacttcaca gcaaatcgcc tggaaaagca
     1621 ccaaggatgg gcagacgatt cacggcattt tgcttttgcc tcccggctac caaaaggggc
     1681 agcgctacaa aacgattgtc caggtgcatg gcgggccgga agaagcgtgg gtcaacggat
     1741 ttcacggtga atggtacgac tggtttgccg tgctcgcctc gcatggttac gtcgtgctgc
     1801 ttcccgatcc gcgtggctcg gatggccagg gcgcggcgtt cacagaagcg aattaccgcg
     1861 actggggcta tggagatttt caggacatca tggatggggt ggactggctc gttacaaaag
     1921 gaatcgcgga ccccggtcgc ctcggcatcg gaggctggag ctacggtggg ttcatgaccg
     1981 gctggacgat cacgcacacg gatcgcttca aagtggccgt agacggcgcc ggaatcgcag
     2041 atgtcttctc gatggcggca acttcggata ttgcacccag ttatttcagc gaatattttg
     2101 gcccgttcgc cgaaaatcgc agcctctacg acgcgcactc gcctgtccgg ttcctcgaac
     2161 actgccacac gcctgtgctc gtgctcgccg gcgaagcgga ttttcgcgtt cccttttcac
     2221 aaagcgagga gttgtacaac ggcctgcacg atctcggcag agaagcggag atggttgcgt
     2281 atccgcgcga gcatcacatt ttcggcgaga aggcgcacca gatcgattcg ctgcagcgcg
     2341 tgctcgattg gtacgccagc catttgcatt aaagacgatt tcgcggcgga aacattcgac
     2401 gtgcccgatt gtttttgttg cgggacagtc ttcgatttca gacggcgcaa ggcgcgagaa
     2461 cggttgcggc aaaatcttgg ggccaaacgg gccaacaata atgcccaact ctgtctgggg
     2521 aagatcggtt acatagaaat cctgcgtctg cacggcttcg atatggcgcg agtgcagcaa
     2581 gctctggcca tttatggttt gtgatcccaa cttctaatgg cgcgagggcg tgaccaccgt
     2641 tcaaatggat agagcgtcta ccttctagga ggtcaagcaa tacaggattt tgttcgaagt
     2701 gtggcgattg cttgtcaaac agtttcaatc cacgcgccca cggacaaggg cctcggcgaa
     2761 agccgaagcc ctttttggct caggcgctgg ttgccttttg ctttgcggat aggaattcat
     2821 tcgcaatcgt cctgaccaag ttgctgaatg aagaatctgg atcaaaagca tttggtaggt
     2881 tggagtacca tgagcgattc cctcggagac gtcaggcagg cttatggcgg ctgcatagag
     2941 ctagccgcaa agagtttccc cctcggcggt ccgcgtggct attcgtctga cggatgcctt
     3001 ttatctatac aattcattgg acagctagga ttgaaacagc cagtattgcg ccagttttcg
     3061 cgttccagcg accacttggg gcagggtcgc tctcaatggg ttccttttta cggaaaaaat
     3121 ggcgaccgcg ccggcttcag ggcgtagcta gaagcggcaa gctaacggcg gggcggaagc
     3181 tccacgaaga atagctaggg aggaagacac ctatgaaaac gaatttgcga aaggccggtt
     3241 ggaggttgct cttgatgatg gtggcggtgt cggcgggcct ggcgattcct gcccacggac
     3301 agactagccc ctcgactggg gcgattcagg gcttggtgca agatccaagc ggtgcagttg
     3361 tctctggtgc tgcaatagcc ctggtcaacc ctaccctggg tataaaccgc tcagttcatt
     3421 ctgcctcgga cggcaccttt ctgttccctc tcctccagcc aggcagcgga tatgaaatca
     3481 cggttgagca ttccggcttt cagaaagcaa gcgtctccaa cgtggctgtt aacgtcacac
     3541 agatcaccgc ggttaatgtc cgcttggtgg tgggcagcgt aacccagcaa gtcgttgtca
     3601 gcggcgcagc cactccaatc catacctcgg atgcgacgct cggcgacacg ctgacgtcaa
     3661 gagttattga gagcatgccg ctgccgacgc gcgatgttct ggatattttg gcaactgacg
     3721 cgggagtggt ctcgcagttg acctctccgg ccgcaacaat cctgcagggc tcggaggcca
     3781 tgttcgtggg cggaaatcgc gccacatcca acaactatat ggtcaacggg atcgacgcga
     3841 acaatttcga attccacact ttggcaacag ggatcgtccc catccccaac ccggatgccg
     3901 tcgaggagtt ccggacagag accagtctgt acgatgccag cacaggattc agtggcgggg
     3961 gcaatatcgt cctcgtcacg cggcgcggca cgaataaata ccacggaacg gcatacgaat
     4021 ttctgcgcaa cacggatatg aatgccaacg acttcttttt caatgccgcc ggaaagccgc
     4081 gtccagtcat gcagcaaaac caatttggat tttccatggg aggcccggtg cccaaagcgc
     4141 cgaacaccta ttggtttttc aattacgaag ggcagcgtca gcgtaacggt gttaccggcg
     4201 cggcgacagg actgcttccc gtgctcccgg acgatcgaag ctccgcttcc ctggcatcac
     4261 tctataacct tccccaaacc gccatcgatc cggtggcaat cgccattctg aacgccccgg
     4321 ggccttacgg cgggaagctc ttccccagcg gttcaggcgc gtcacaaggg gaactcggat
     4381 catttgcgtt ttcctcccct gcgcaactga atagcaatca atacaacgcc cgcatcgatc
     4441 acagctttcg gatcggtggt atggacaacc agttcagcgt ttcctacttc gcgaatcccg
     4501 atacgttcac aaacagcagc ggcgcaaacg gaagcacgat tggccagccc tatcaatacc
     4561 ttctccaaaa cgagacactt tcagttcagg atacccaaac cattcgcccc accctgctga
     4621 acagcatcaa tgtgggattc acatacaaca aacgggacat tggggcgatc cacccgctga
     4681 cgctgaacga ttttggaatg acgcggttta attcttcgat tatcgaccgg ggccccaatt
     4741 tttcatttgc ggaccagatg aattgctgtg gcgctagcgt ttcggtgggt gagacgcagc
     4801 acaacgaatc gcaggatttc cgggatatgc tgtcctactc tcatggcgtg catttcttcc
     4861 gtttcggctt tgaagcgcgc acccagcagt tcaatttcgg ctccccgctc gatcctggct
     4921 cgcttttctt tcagggcggc ctggccgatg cgctctacgg cccgccgagc gatcccaatg
     4981 ccgatctttc cattcgtgat ttcctcgtcg gagcgccttt tttcataagc attggcagcg
     5041 gactgaacga ttttgagtac cgcgcccatg attttgggct cttcggtgag gacgattatc
     5101 acttgacgag tcggcttacc ttgaatcttg gtttgcgctg ggattattta gggaatatca
     5161 ccgagaagca tgatttgatc tcgaatttcg atccctcctt gctaaccccg ctggccatgc
     5221 aaaccggcgg gccgggtctg caggacggat tcgtgattcc ctccggtgcc ccgaagtttg
     5281 gtacccccgg agtctcgaaa tccacttaca acaaccagcc gctcaaggat ttcgagccgc
     5341 gaatcggttt tggacttgac gtgctgggga atggccggct ggcgcttcgc ggcggattcg
     5401 gcttgtattt ttctcggatt gggccttttg gcctgctgca gaccatcagc aatccgcctt
     5461 ttgcgctgag cacggcgatt gtcaatacct cacaggcaca gctcctctcc gatccgttcc
     5521 ccacgcttcc gctcccaagc cagtttccta ttttcactcc ctttccgcag ctcacttccc
     5581 tcaactctga cggctcgccc aattttacgg gtccacagtt gaatgtcatc gagttgcagc
     5641 gcgattcgca cccgccgtac accgaacagt ggaacctgac cacggaatac caattcctta
     5701 agggctggac catacaggtt ggctacatgg gttcccacgg cattcacctt caggatttcc
     5761 agggcttgaa caatgctttg ctccgcaatg ccaacaatcc tggcccctcg ggtctggacg
     5821 tgaattccgc gcagaatcgt gaatcccgtg ttccctacgt gggcatcagc tcgacgggaa
     5881 tgctggcgct cacggacgcg ggagcttcga gctacaacgc tctgcttgtc acgctgagtc
     5941 acgagttttc tcgcggcctg tttctcaaag ctgcctacac cttttcgaaa accatcgaca
     6001 atgtacaagc tcaggcaggt ttcgaaccca ctgtcggtcc aagcggaaat caatttctcc
     6061 cgcagctcaa tcgaggcatc tccaatttcg atgtcccgca tcgcctggtg ctcacctacg
     6121 tctacgacat cccgggaccc agaagcggat ggcaagccgc ccttctgaat cactggactt
     6181 tgtcggggat caccacgctt caatccggct tcgcgggaga aatcgatcag ttcaccgcag
     6241 gaaactccct gtcgggaacc gatggatacg gcatcctgac accgggttgc gcgctcgtga
     6301 atggaggcaa tgtaagcgcc cacacacgcg attacctcca tagctcttgc gtatcgacga
     6361 ccgctgcgct tctgagcgga agcacgttcg gcccgttgtc tcccttcgag ggacctggaa
     6421 accagaccta tacgatcgat ccgagcaacc ccgcagcgat cggaaatctg caaggcccat
     6481 ccactcgggg cgcatttttt ggcccgttcg agacgcgctg ggacatggcc ctggccaaaa
     6541 ctattcccct gcgggagctc ggcgagcagg gaaatttaca gttccgcgcc gaagccttca
     6601 aaactttcaa tacaccgatc ttcagcaatc cgcacagcgt ggccggattg tcgagcttcg
     6661 ggagaattac cagcacaatc gacaacactg gccgtcaatt gcagctggcg gttcggctga
     6721 actggtaatt gggtggggga gtggccgtcg ctcccttttc ttcctggcga gtgaaggaga
     6781 tcaagatcat gactaagtgg gacaactcgg tgtgtatcgt ctcgttggct ctcacctgct
     6841 tgctttttgg cggggcttcg cttgctgcgc ggaccgtgcc gcctaatttt tacgacagca
     6901 tgcagtggcg cctcatcggc ccctttcggg gcggtcgcgt ggaagcggtg acggggatcc
     6961 ctggcaatcc gctcgtctac tattttggtt cagttgcggg cggcgtgtgg aaaacggtcg
     7021 acggaggcca cacgtggctg ccgctgttcc aaaaagagct cgtttcctcg attggcgcct
     7081 tagccattga tccttccgat ccggaggtaa tttacgtcgg cacgggagag gcctgccctc
     7141 gtggcgatat gtccttcggc gatggtgttt acaaatcttc ggatggggga aagacctggt
     7201 caaaccttgg cctgaacgat acacggcaca ttgccaagat catcgtggat ccacgaaacc
     7261 cggaggtaat ctatgtcgct gcgctcggct ccgtgtatgg gcctagcgaa gaccgcggag
     7321 tgtttcgttc cagtgacgga ggaaaaacct ggcaaaaagt gcttttccac gatgccagca
     7381 cgggggccat tgatctggcg atggacccac acaatcgaca tgtactcttt gccgccctct
     7441 ggcaggttcg acgaaaacct tgggatttta ccagcggcgg tccaggcagc ggactctacc
     7501 gctcaactga tggcggggat cattggacag aaattaaagg ccatggtctt ccggtcggcg
     7561 tgctcggccg gatcggcgtc agtgtgggcg cccagtcgaa tcggatttat gccatcgttg
     7621 aggcgaaaga tggcggcctt taccgttccg acgatggagg gacaaactgg aatcgggtaa
     7681 acggaagctg ggatttgcgc ggccgcccct ggtattactc ccatattttt gccgatccca
     7741 acgatgttga tgaagtctat gtctttagtt tcggggcgta tcgctcagtg gatgggggaa
     7801 agacgtttgc tgcgctttac acaccgcacg gagattacca cgcactgtgg attgatcctc
     7861 gggactcccg gcggctgatt gtgggaaatg atggcggtgc cacgatttcg gtcgatgatg
     7921 ggacgagctg gactacggaa gacaaccaac ccaccggcca gttctaccgc gtagctacgg
     7981 acaatcgatt caactactat ctttacggat cccagcaaga ccggggaacc gttgccatca
     8041 ccacaaggag tgatgaggcc cttattggtc agaacgactg gttttcggtt gggggagggg
     8101 agagcggatt tgttctgcct tcgcctctca actctgacgt tgtctatgcg ggaagcctgt
     8161 atagcgcgtt cacacgcttt gaccggggtg ccgcgcaatc gcgggatatc tcaccttggc
     8221 ctctcaatga actgaatctt cccgcgaaag atgcccgcta ccgcttcggg tggacggcac
     8281 caatgctgat ctccccgcat gacccgcaca ccctgtacat cggggcgcag gtgttattca
     8341 agactcggga tgaaggcaag acctgggatg tcatcagccc tgatctgacg cgaaacgata
     8401 gatctaagca gaattctgcc ggtggcccga tcacccagga caacacgaca gtcgaatact
     8461 tcgacatgat ctccacaatc gcggagtccc cgcttgaatc gggactgatc tgggtgggaa
     8521 cggacgatgg atggatccag gtcacacgag atgccggtaa aagctgggta aacgtcaccc
     8581 cgaaaggaat gccggattgg agcctcgtga gtatgatcga gccgtcgcgt ttttctgtcg
     8641 gtacggccta tgccgtagtc gatgaccatc agctcaatga cttcaggccg cacgtttacc
     8701 gcactgccga tttcggccgc acctgggtat ctatcactga aggtttgccg gccgattcct
     8761 acgcgcacac cgtgcgcgaa gatccagcgc ggcgaggctt gctttacgca ggaaccgaaa
     8821 ccggagtgtt tgtctctttc gacgatggga gtcactggca atctcttcaa ctgaaccttc
     8881 cgacggtgcc ggtgtatgac ctcacggttc acgaaaatga tctggctgta gccacgcatg
     8941 ggcgtggttt ctgggttctc gacgacctaa cccccttgcg acaagccgca gaagcttcgg
     9001 cgagcagcga tttttatctc tatcgacccg agcccgccta tcgcgtgcgc ggcaataact
     9061 tctatgcgac agtctcttcg ggtgcgccca gcagccctgt ggccggacag aatcctcctg
     9121 caggcgccat actcgactac tatctcagcg cacagcctca gacatcagcc aagcttgaga
     9181 tcgttgacga taaaggaaga attgtacggg agttcgacct gcgttcggct gcggtctcgg
     9241 caagcggaaa gcaagtgctc atcacagcag gcgctgtgac cctcgcggac ggccaggagt
     9301 ataggactga tcctacagtg caccgcggcc tcaaccgctt cgcttgggat ctgctatacc
     9361 aagggaacga gagtagcccc aggggcatga gagttcccga gccgctcgta gtaccgggga
     9421 attacgtggt gcgactcacc gtcgacggca aaactcagaa gcagccgctc gtggtgcgcg
     9481 aggatcctcg ggtccaggtt tctctcgccg atctgcaggc gcaatttaac ctacagagca
     9541 agattgcaga tcgtttgcaa gaggccgcag tcgctgttgc tgaaatgaag caggtgcgtg
     9601 gtgagcttgt ggctctcggc aagcgactgg atgcatcgag aagtccagaa aaacttaaga
     9661 aggccctcga caacgtagag agcaaaactg aagatgcgga aagatccatc acaggctgga
     9721 aagcggcggg cgatggttat gcgctgaact acccgccggc attagatgac cagatcaatt
     9781 ggttgttcat gtttgcggga gcaggcgacg gagcacccgg gcagccattt ctcgatttgt
     9841 acgaccagct gagtcgcgaa ctggcggctg acctgggccg ctggcatgaa atcaagactg
     9901 ccgatcttgt tcagctcaac gagttgattc gagagaacaa gatacctgca atcgccgcct
     9961 tcgcccctcc cccgagccat taacccgagg agcttagtac gcgaatggat tatccgggaa
    10021 ggtcagaacg ggacgatgcc ctccggcgag tgcatcgctg aaatgcagga gccacatacc
    10081 gatcccggaa gctccttgca tgtacccggt ttgcgccacc gcaatatccg ggcggacccg
    10141 ggtttccacc tgcacccagc ggtaccgttt gccgtcctcg tcagccagtt cgacgatttg
    10201 ttcgctgcct ttcttggccg ccctgaacca tctgcgatca tgagtgacca ggaaagcatc
    10261 gtagagaaac tccgattcgg ccgcaatccc gcagcacatg ctaacgttat gccaaacacc
    10321 gggagtgacc gcttttgccg ggcctccgtt ggctacgacg gcttccgcac atttctccac
    10381 gagatcccgc cattgggcat cacgagtcac ctggtagagt ctatagaata gccgtgctgt
    10441 tccggccggt ccgtgacacc agcccaagta aaaaagatct tggtgctcgc tatcgtggta
    10501 gatcatgcag tagcggttat tcaggtcggc gatggacagt aagtacttcg cgccagcaag
    10561 cgccgcatcg aggaacttct cttggcctgt ggcctggtac agcgttgcta agaaataaga
    10621 aacaccggcc gtgccgtgcg agaaattggg catctcccgg ggatagctgg gatccatcat
    10681 ccacatcagg ccatcttgtt ttcgctgagc ggtcgcgatc aagaaatcac cggcgcgcgt
    10741 cgcgagctgt tttgctcccg gagcctggag gtggtcggcg gcccaaagca ggaagagacc
    10801 ggtgccgctc gaacccgcga tgacgtcagt gacatcgttc catttggccc ctgtgggtgt
    10861 catttgcgcg cgtttttcca gccagcgcac gcaaaggaga gcgccgtcgc gataacgggt
    10921 gtcatgcgta atcaagtagg cttccccaag cgtgaagccc gaaccggcca ggccttcata
    10981 caatccggtc ccatctttct cggaaatcga tgccagaaga gcgtcggctc ccttcttgcc
    11041 cttttccagg aaatcccggt cctgcgtgta gcgataggct tcaagaaaaa acagaattgg
    11101 acctggagtg ccagcataca gcgtagtgtt aacactctcc ggatttcgag ggtccgcagg
    11161 ccagacgatc ccattccccg tgttcgagcc gttttcctcg atccagtgcg ccgccccaat
    11221 ggctgctcgc tggtacgcgt ctcgggttcg tcctcgcgcc ccccaggaaa tggaaagcgc
    11281 ggcgcaagcc gccaccgctg caattcgcca atgcttgctc attgagttcg ctcctcggca
    11341 agaacggttc ggactttctg accccgcagc gttttcagta agggaacggg ttatcgggaa
    11401 accggatgac tggccggtgt tgtccggcga gcgcagcgct gaagtgcagc agccacatac
    11461 ctatccccga ggccccctgc atataggacg tttgcgcgat ggcatagtca ggccgaacga
    11521 aagtttcgac ctgcacccag cggtattgtc catcatgcac atcggctctc cgcagtaagt
    11581 cttcactgcc ttctttggcc gcctggagcc atttgcgatc atgcgcaacc agataaacat
    11641 tgtaaaaaaa ctccgcctcg ccggcagtgc cgtcgcagat gctgacgtta cgccatacgc
    11701 ccggattcgg gacttgcttg ggctgccctt ggcgccaaac ggtgagcgtc gtggccgatc
    11761 ccgatggtat ggtcgtaaaa gccttctctg gcccaccaaa aatccgtgtg gctgccgcgc
    11821 acttttccac taattccagc cacttcggct gttttgtaat ttgataaagg cggtagaaga
    11881 gcgcggcggt tcctgccggg ccgtgcgccc aatcgagata aaagagatgc gtgttgtgat
    11941 tgtcatgata gacgaggaaa tactgctctg aggaattggc gatcgcgatc aggtgcttgg
    12001 cgcctttgcg cgcggcctcc agaaactcct tgcggccagt gacttgataa agcgtagcca
    12061 gaaaatacgc cactcctgcc gtgccgtgac agaaattcgg catctcgaat ggatagctgt
    12121 tgtccatcat ccacttgagc ccgcccccgg gttgcggctg gcccatccga atcagatgtt
    12181 caccaacccg cacggccaag gcacgggcct ctaccgcctg aagatggtcg gccgcccaaa
    12241 gcaagaatag gcctgtcccg ctcgctccgc taataatatc cgtgacccca ttccactggc
    12301 ttccatccgc agtattcgtc gccttcgcct caatccagtg caggcactgc agcgcacctt
    12361 cccgatactt ggcatcgcga gtgatcaaat aggcctcgcc caaagtaaaa ccagaacctg
    12421 caaggccagt gtacagacct gtgtcgtcat tcttggaaac ggatgcgagg agcgcatcgg
    12481 ctcccttgcg cgcctgttcg aggtacacgc gatttcccgt gtagcggtag gcctccagaa
    12541 aaaaaagaat cgggccagga gtgccggcga acagagttgt attcatactc ttggggtctt
    12601 cgggattggc ggcccagacg attcccgaat tcttggctgt gccgttcatg cggagccact
    12661 gcgccgctcc gagagctgcc ttttcataag gggagtccgc agcctgtgcg ggaagaatgg
    12721 aaaccatcag aacgatcagg acgactgcga cgccttggag cagatgtttc attgatccac
    12781 ctcgctgcac tgctgctatt tgtgaaaaac cagccggccg ttcttgacga cgacgatgat
    12841 gtttctcaag ctcttgatgg acgcaagcgg gtcaccgtga acgacgatga tatcagcgcg
    12901 gcggcctact tggattgtgc cgaaatcatc tcctcggccc agccaagagg ccccacttcc
    12961 agtcgccgct cggatgatct gctcacgagg aataccggcc tgctcgtagg cctccagttc
    13021 atcaaagagg ctgccgtcat cggtgcctgc caagagaggt actcccgcgg ccacagcatt
    13081 gtgcacgaac tgcgacatcc gttcgtagct ctgctcccaa gtggcaactt cctcgagact
    13141 gagttgttta cgcagggaag gagcgggaga ctggatcgac aaggtcgggt caaagccaat
    13201 tttcgcccgc aacatgcggg caatcaattc ctgggcacga gcgccgttgg gatcgatgtt
    13261 tcgccagtcc gcttgcattc gcgccacgag gtgccatgta gcatcgagag tgcggtcggc
    13321 tccttgcggc tgggcgcttg ccggcagaaa ttccggccat acgcggatgt ggttgagacc
    13381 gtcgatgccg gcgccgatgg cttcggtcca gctggtccgc actccgatgt gtcccgtgac
    13441 ccgcaggtgg tctcgatgcg cagcctcgat cacggccctg aggacttccg gcttcagcag
    13501 aaagtaaacc ttcaacagat ccacaccctg agccttgagc gagtcaacca ggggcttggc
    13561 ttgttctgcc tggttgatct ggacggcaac catcggatgg taggttccga atccatcaat
    13621 caaaggccca gcggtgtaga tgtccggacc cggctcgaga cccagcgcga tggcttcctt
    13681 gaagtttaag ctatccgcca gatccgctcc cggatctcga atgcaagtaa tgccccggga
    13741 aagcaagcgg gagccgtcaa aagcgtccca gaaatggtag tggttatcca tcaggccggg
    13801 aagaactgaa tcaccctgcg catcgaaaac cggaacgccg gcaatatcac cggggcggaa
    13861 cgccccacgc tgaatcttga caatgcgccc tccacgaatg aggacgctgg cgtcagcaat
    13921 cggcggacgc cctgtaccat cgatcaaatg agcatggatg accaggagat cggcgggcca
    13981 ctgcggtgga acttggaacg ccacgtgaaa aggaatcggc gcgagcgctc cgctagaaat
    14041 atccaggcgg tagagtttgc gggctgcgca aacgatcagg gtgttcgcgt ctggtccgaa
    14101 cgtgatttgt tcatcctgat aggcactacg gaaggccact cggggctttc cgttgccctc
    14161 cgcctggtcg acgcggatct cgtctgcccc gttgccggtc tccacgtacg cgactcgcac
    14221 cccatctcgg ctccaggcaa cgctggtgtc gtcgaaggga gtgtttgtaa gacggactgc
    14281 ccgcctcttc tccacgtcca tcatgtagag atttgcgccg ccataaagcc ctgaggtcag
    14341 aagcagacgc ttccccgaag gcgacggcgc aaaagaccac tcgtgccacc cgcgagtctc
    14401 ggtaatctct tccggtgcgc ctccactggt ggggatcttc caaatatgcg catcgccttg
    14461 accatggcgc accgtgaaga aaatgtcgcc ccctgcaggg tcataggcga tttggccgat
    14521 cacgccgtca gtttcataaa cgacggccca agttccgact gggatatcgt agaccaccag
    14581 ataactgcga tagttcacct catgcgaaaa cgccagaaat gcgccatcgg gcgaccaagc
    14641 cgggaagcgg tcccacccga tacctgacga aattagttgt cccgtgccgc ccgcagcagg
    14701 caagagacag atcctaccca gcacactgac ggcaatccgg gttccatcgg gggacatggc
    14761 cgggtctctc gcgccatggt cgatgaccac cgatcctgct tcggcccaaa gcggtggggc
    14821 aaaaaaaacc gcagagacca ccgagagaac aatcccgaga aatccagaca gacaggccgg
    14881 aagagagcgc agccgcaaac tcccgcaatt atcagagccg acgaaagagc ttaagcggga
    14941 gctgcggaat ttggatctca cgacctagcc tcggacgacc tggatggtga cagaaacttc
    15001 tgctgtgggt cgcagcgacg cagccacgct gcagtacttg gcattcgaga gctcgactgc
    15061 gtgcctcaca gcatcttcag agagatttgg gcctttcacg acgtgatgga ctcggatagt
    15121 ttcataaatg cgaggagctt cctgacggcg ctcaccgtga acctcaatcc gatagtccgt
    15181 tacttgctcg cgcttcttag tgagaatatc gacaacgtcc gaacccatgc atcctccgat
    15241 cgcgagcagt agcaactcaa ccggcgttgg cgccgaactc ctttcgctgt tggtctccat
    15301 aaccagggcc tgcccgcccg gactgatccc cacaaaaaaa ccgttgccgc cgtagtgcac
    15361 ggtagcgttg atctctgcca ttctgctctc cttacctgat gttagggttg atggacgacg
    15421 tgacgagcgt ataatgtggg ttcctgcact agctgtccaa tgaatttgat agatgaaacc
    15481 tatgcttctt aggaaacggt tcgggcgcat cataatagcg ggtcagtcct caaggaggtt
    15541 ttgatggagt tgcgccagat cgaaattttt aaaatgcttg ccgaggaact gaacttcacg
    15601 cgagccggca gaagaatcca ctgcgtgcag tccaacgtga gtgtgcagat acgctctctt
    15661 gagaaagaat tcggagtcca gttatttgag agattaggtc agagggtgcg tctgacagcc
    15721 cagggccaaa cgttcctgcc atatgcagaa cgcattttac ggctgataca ggaagccgcc
    15781 gcagtcacga gaggtggtga gaatccggca ggaactttgg tcatcggcat gccggaatcc
    15841 gtgctcacct atcgtctgcc gcccgtactt caagtctttc ggagcaactg gccgcacgtc
    15901 gaactggtct ttcgcggaat cagctccctt gagctcattc cctgcttgga gcgcggtgac
    15961 ctagatctcg ggctggtcat cgatgatgcg atccggatcc cgggaattca tgcagaggtc
    16021 ttgtgcaacg agccgctagt gctgactgtg cagcctggac atccgctttt gcgcgcgccg
    16081 attgtgcaac ccgaggatct cggatcctat acttttctgc ttacggacca gggttgcgct
    16141 tatcgctcta aacttgagac agcgctggcg cgcgtgcacg ttcggccaag gacggtaatg
    16201 gaatttacaa gcgttgaagc gattaagcaa tgcgctgctt tggggatggg aatcgcctgt
    16261 ctgcccaaga ttgttgtgga gcgtgagctc gctagtagca aactggttgt tcttccagca
    16321 gcgagtttcg aactgacgat gaatacgttg gcggtctggc atcgggacaa atggatctct
    16381 ccagcgatga aggccttcct ttcgctgatc cggttgcacc tgggcagcgc atctatttct
    16441 aatgtcccaa caaaaagtcc ggcgacgatc attgacgggc ggaagcctgc agccagtgcc
    16501 gcgggaaaga accgtgaagc ccgaatgact tctgggagac accgcggagt cgcaccgtag
    16561 cttttgctcg gacatcaatt cgggcatccg ggcaatctcg ctgccattct tctagacgca
    16621 ccatacagct ctgccagatc ggaatcgagc atcactcttt gcccgcgaat cagcaaaatg
    16681 cgggattcga tccgttcagc ccgtaccatc gcctcttttt tggtcatcgc cctttcttga
    16741 gacgtcaact ttggcatttc aactcgaaat cacatttgtg atttcaagtt gtttcgatcg
    16801 ctgtggttcg ttccgtcctc gattccaatc cgactatcaa ttcgtagtac ggtttcgtcc
    16861 tgaaataatc tgggataata aatgcatgtg caaccgctac cgattgactc acgcccaacg
    16921 ctacctcgcc gaacgattcc aggcgtggga tgactttgac gagactcccc gctacaacat
    16981 cgcgccgacg cagccagtcc tgaccgtccg ccgcgaacag ggaaagaaga ttcgccgatt
    17041 cacgatgatg cgttggggtc tgataccgca ttgggctaaa ggcacaagca tcggcactcg
    17101 aactctgaat gctcggtcgg agaccgttac gagtacaccc ccttttcgag agtccatcct
    17161 aactaaacgc tgtctcatcc cggcagatgg tttttacgaa tggcgcgcga tgggccgcat
    17221 taagcagcct ttttgttttg aagtcggcga gggagatgtc ttcgcgtttg caggtctatg
    17281 gggccagtgg gagggtcccg agggcgaggt gatcgagagt tgcacgatcc tgacgaccgg
    17341 gccaaattct ctcgtagcgg agatgcatga ccgaatgcct gtgattgtgc cgcctgataa
    17401 atacgaaatt tggctcgatc cagacattac caattttgat gcgatcaaag atattctcaa
    17461 gccatacgac gcaggtcaaa tgcggcggta tccggtgagc acgaaactca acaactcgaa
    17521 gaatgaaggt gcggaatccg ctgcgccggt cacattggac gttccgttat tctaggacgt
    17581 ttccaaagac caaacatatt gcgcgggccg ttcaagctga ggagaaaacg caaatgacac
    17641 aacagatcgt cgatgttcac atcaatcctt ccgaagaggc catccgtctc gggccggttg
    17701 cggttcgctt cctgatcact ggagagaatt cgagtggcag tatcgcagca tttgaggtca
    17761 ttgtcccggg cgcgcagcgc ctaacagctc cggctcatag ccacgatcat tatgaagaga
    17821 cgatctacgg catcgccggc atattgacct ggacagtcga tggcaaacaa atcgatgtcg
    17881 cgcctgggca ggcgctatgc attccgcgtg gcgctgttca ccggttcgac aacaatgcaa
    17941 gtcagcatgc aaaggcgctt tgtgtgatca cgccagccgc gataggcccg cagtttttcc
    18001 gcgaatgtgc cgatgtgctc cgcgcgacgg ctggcggtcc gccggaccga gcaaagttgg
    18061 tggaaatcat gcgtcgccac ggtctcacgc cggctccgcc tccggctaac gcgtagctct
    18121 ggatgccttg cgcgccgcat cagcgctcgg agagaatgga accatatcaa gacccagttg
    18181 ttcttatcgc gctgagcgcg gactcttgcc agagctcggc agcttcgttc cagaacatct
    18241 ttgcctcctg atcattagcc gcgccttttg gctgcatagg attctcctta gcgaaaaaag
    18301 tgaaggatgc ggccgcggtt cttgcccgat gaagaaaaag gcgcggcgaa aaacggcgga
    18361 tgcgagctgc tactcgctct tattcacgac gcgaccattg aggggctgga cgggacgcac
    18421 gtcgtgcggg tagagctttg caaaggcggc aattttgccg ggtgcgattt gcgcaagcgt
    18481 tttgagcacg gcttgtgtca gaggacaggg tgcatggcga gggggtaccg gatcgccgat
    18541 aaccacatgg atatctagcg ggccctcgtc gcttagcccg gttgagaaac ccggcaacac
    18601 gattcatttg aaccggagaa aactttgagc agttcatctc actcgccgtc gcgggactcg
    18661 cggcacgcca cgcgcatgcc gcaagccaag tgcatcagcc gtttgcgatt cgcctatttc
    18721 ggcttgagca cgagcttgtc agcagcgtgt gcttgcaccg ctccccttga aaaccacagt
    18781 ggaaaatagc gtccgtcgcc ccacagaggc agcaagtcgg aataatgagg ggcttccggc
    18841 tgcccgcttt gtccgggaac gttcaccgcg acggatttat cccagtcgct cagatccatg
    18901 atttcgcgat agcttgcgcc tgcaacctgc cggaagtcac tgccccgaaa tcccgtcgca
    18961 tcgacagtgt agtcgtcccc cggacgtgca atcgggccaa gatcaaacag gattttgccc
    19021 tgcggagctt cgtccaaagg atggcggaac tccacgacat gaatacgtcc ccaagaccat
    19081 ttcgccgggt ccgggccttc gagctgcgcc aatttctcgt gcgccgattt cagcgcatcg
    19141 agcagcagct cgtttcgtgc tgcctcggga tgcccgccaa agatttccgg acttggatga
    19201 gagattgaat caagcacttt cgccaccggc cagccgcgca tcatgttcca caactccttt
    19261 gggacagcgc gacggctgac cgcggcagtg acttctggca gccaaagctc atagagcgcg
    19321 gcagccgccg aatctctcgc caccagtccg ttccaaccga gcaacagtcg cacgtctgga
    19381 tcactgctgt ttcctgctgc ttgtcgcaga agccctatga gctcaacggc tggtgtcgag
    19441 agcacatcgt tttgcagctt ctccatgtcc gcgactgtta gcttatgccc actcgatttc
    19501 gccgcttcga gtacggcggt gacgcgccgg aaacgaaatc caggggccca ctcgtagccc
    19561 acccggtacg gataattcgg cggaatcatc ttgtggtttg cggtcgcgac gaatcctctt
    19621 tctggattga acgaatgtgg caattccgcg agcggcagga aaccgttcca ctcataaccg
    19681 ccgtcaccgg gaacaggaag caatccgtac cagttcttcc gcacgggcgc aagtcccaca
    19741 gagtattcgc caatgttttc ggcgcgatcg gcatacacga tattttccga gggcactttc
    19801 catcgcttca ttgcttcgac gaactgttgc cagttctgcg cgcggtccac ggcaagcgaa
    19861 cccagatagc ccgccgtccc cggctcagct ccaacccatc gcagcgccag tgcacgcgtt
    19921 ttgtccgccc acagcaccgg cccgtggcgc gtgaatttca gatcgacgac aacatttgtc
    19981 tggccgcgca ctccaaacgt ttctttttcg acgcgcattt tcgcccagcc ccgctcagtt
    20041 ttgtactcga gaggattcgc tgcattcagt ttctcgatat ataaatcctg ctgatcgagg
    20101 ccgaaaatcg tgaatcccca cgcgatattc tgattgtggc ccacggccac gccgggaagg
    20161 cccggctcgc cggctccgat cacgtcccag cccggcgcaa ccaggtgaac gatgtagcgc
    20221 agcgacggct cggcaattac gcgatgcgga tcattcgcca cgatcggttt gccgctcgcg
    20281 gtcagagcgc cagaaaccgt ccaatcattg cttccttcaa gagcgcttgc gggaaattcg
    20341 attcgcgtgt cgctgcccac cagattccgc agcaattgcg gtgagagtcc agcgaaattc
    20401 acgccgggtg caggatcgag ttgcactgcg ggatcgagcg caaacagttt tgtcgcgcgc
    20461 gcagcgccaa tcctggcgac tgcttccgcg tgatacagct cggcggtcga gtttccggtc
    20521 atggaaaacg ccgccatgcg attcagacaa tcttccggcc tccacggctc gggtttgaat
    20581 ccggcaatct gaaattcaat cggcaagccc ggaccggagg gcctcgttcg ctctgcgatg
    20641 taagcgttga tcccgtcggt gaatgcccgc aggatttcct ttgtgtccgg cgagtagctc
    20701 ttgtactcgg catcgatgtc gccggagtag cgcagcagac gggcattcac gtcgcgctgc
    20761 agggcactcg gccctagaat ctcggccagc ctgccctccc ccgaccgctt ccatagctcc
    20821 atctggaaca ggcgatcctg cgaaacgaca tatccctgcg cgaagaagag atcgtgctgg
    20881 ttctgagcgt aaatgtgcgc cacaccccag cggtcgcgca gcaccgtgac cggcttttcc
    20941 aggccagcga tctggagcgt cccgcgaatc tctgaaatag ccttcttagc ccgcgaatca
    21001 agttttgcgg tcgagctttg ggtcagtccg cggatcgcaa attgccaaag agggccaaca
    21061 gagaagaaaa cagtcacgaa aaagagcgca gcacgtcgct tcgcgttgcc cacggtgcct
    21121 cctcaacatc caaatgaatc aatgcggcgc gacaataaag aatctgccct ccagagtcaa
    21181 ggaacgcact ggacaatggt ccagctgtgg cctcccggtt gcagcgctcc gccccactgc
    21241 aaaattgctc cgttgaaccc gtgcaagtcg gggtcggcac ttccaatttg ccgctaaaca
    21301 catccctgat gaagagggac tgaaaggtgt tgcatgcgac acagagagca cgatccggtg
    21361 catgatgagg ccgatgtgtc gtggtgtcgc tgctagacgg tagaggattt atccgcgcaa
    21421 aagactattg ttgtgaaaag taggcctcaa tccaaaactt caaagtattg tccgcccgct
    21481 cagcaggccc aatcaaatta catctcaatt cttcgttgta ccgctcgcgc aatatttccg
    21541 gatcgagatg ctgcattcgc gccaagtact tcacgtcttc ctggtctttc gcgctatttc
    21601 gactcaactt cgagagaacc aaatcgtaag gatcaaggac aagaaggcgg agattcttga
    21661 atctattagg gaatagctgt tttaaccttc ggtcgtagtc ttcgggataa gtcgcgattg
    21721 tcacgtaatg gacaaacact ttatgtttct tcgcgagagg cgagccttct ccggcaatgc
    21781 tctccaaatc aggcgcattg ttggattcga tatgaatgta atctaggccg tttgtgctgc
    21841 gcggcagacc atacatcgca actgccgcga atccgccgat gcagtgaaga tcaaggcgcg
    21901 caggaagaag attatcaagg tcttcgagaa actcccgcca cggtgaaacc aattcaggcg
    21961 acatagcgca gttggtcaga ccgcagatcg ctcagaagat tccattgccg cgcttcttcc
    22021 gaccgatttt ctcgaagcca gcgacgctcc ggctcattta attcacggca gaaagtatct
    22081 tcttttgcca atttgctctc ggtaaggacc tgttcaggcc cttcgaggtc ggcattatga
    22141 ccggcgatct tcgcaagagt cacagtgaag cccaagcggt tttgcagatt gttcattcgc
    22201 gcttgtgccg ttagccagcg aaagtccata tccggatagc ggaaaacgag ccacggcaga
    22261 ccctcagcaa cgcgggcctc aagattgttt tgtttcagag ccagcaataa aaactgtgcc
    22321 ggatttacct tctttgagcc tcggcgaaga tgcgcgaagc cgggataatc caatgcagca
    22381 agttcgcggg ccaggaaagc cggagttgga ttcaacatgg tagtctccga gacgggtaaa
    22441 acgacaggcg atacgtggta ttcccgtatc aattttcggg caagttcagc agatggtttt
    22501 cgtgcaccgc tttccagcat cgagtaataa gcctgagtca cacccagacg ggcagcgact
    22561 tgttgctgag tctgcccctg tttgaaccgc gcctgtctta attgttctga agtcatgtgg
    22621 atgttataac tcattattgt tatattgtca aggccctgtt atgcggcgta ttgacattgg
    22681 taccgtttgt gttaccatcg aatatgatcg aagtacgcga atacctggat cgtcgtggac
    22741 acagcccttt tgctgcgtgg tccgacagct tgaacaaaga agcagccgcg aaagttgccg
    22801 ctgccctcgc acgaatgcag caaggcaatt tttctaacgc gaagggcgtt gggactggcg
    22861 tttacgagta caggattgat tttggtccgg gataccgcat ttacttcggg aaggatggcg
    22921 agcggctggt gatactcctt ggcggcggca cgaaaaaacg ccagcagacg gacatctcga
    22981 cagcactcgc ccgatggcag gactataagc aacgaaaagg gacagagagg taaaaccaat
    23041 ggctttgacg cgagatttca aagacacgat ccgagcgcgc gttcaacgcg atgccggttt
    23101 tcgcgaggcg ctcctgcgcg aggggatcga aaactttctc tccggtgatg tggaaaccgg
    23161 gaagatcatc cttcgcgatt tcatcaacgc cacagtgggg tttccgaaat tgagcgaaat
    23221 cacgcattgt tctgcaaaga gtctgatgcg aatgctcggg ccgcgtggca atccccaagc
    23281 gaaaaatctg tttgagatcg tcaagtactt gcaggagacc gagggcgtac agtttgaagt
    23341 ccggcctagg cgccgcgccg cacgcacgaa gcgcaccgaa ttgccagctc cacatcgtat
    23401 gacgcgacgt tctgccttct agcccgcagt tgctggggaa tccgatgatc gaatctccat
    23461 ccgttgccaa actggaagac tcgtaatcag atggtctaat tcctgcgccg actcggcagt
    23521 tacgatcagc gccagggcaa cggcaccact catgggcccg cctgccagca tctttctatc
    23581 tttttgcagt ttgtcgcatc gctcaagagt agggaggatg agctcctcaa taaatccgtc
    23641 accggggctc attggacgag ccgaactgct caaccgcaac tgcaccaagt attgcatcat
    23701 atctccttcc catcacatgc ccttctgaaa tggtgattcg tcggatattg acgccacaga
    23761 ttaagatggc tgtttcttgt acgcctgcag tatggatgct tacgcaccgc gctgactgga
    23821 ttctggtggt cagcaacccg taagtcatga tcacactgaa gagtgtgcac gatcagaccg
    23881 gctcactttt gggggttcac tccacggctc cgccaaaaat ggggcaagtg tgccgaatgc
    23941 aggcgacact ggttgtagta gggggcggca acagactgaa ctttgtccct tccgcgccgg
    24001 ggagattctc aactgtgccc caataatcgt gatgtgaaag attcatttgc caatccgatc
    24061 tcggcgcggc agccgatgtg cggagccaaa gggattcctt cagcccgcca tcacccttcc
    24121 gaacaccatc cgttgcatcg agcggggcat gagacggtcc atgaacacgt tcgccttgtt
    24181 caaaaaacca ggcaccacac tcgctttgtc gcgcatggca gccagtacgg cagttttggc
    24241 tacgacttga ggtttcattc tcagcgcctt gagaacgagc gaggatttct ctccggcgac
    24301 agcgctaaag gcggtcgctg tcgcacctgg cgagacagtg gtcaccgaca caccgcgagg
    24361 tgacagttct tgatggagcg cctcgcccaa gagaagcaca taccccttgg tcgcggcata
    24421 ggccgcatat ccaggcaccg cctggtaccc cagcacgctc gccacgagca ggactcggcc
    24481 atgccctcga ttggccattt cccagccaaa gatgtgtgtc agttcggtta aggccatgac
    24541 gttcagccgc atcatttctc ctagcttctc gacagactgg tcgaggaagt cgccatacgc
    24601 cccaaatccg gcgttgttga cgagaacctc gacctctatg ccgcgcgcat cgagctgcgc
    24661 cttcaattcg gcggcagccc ctggctgtga gagatccatc cccatcacga caacctgcac
    24721 cgattgctct ttccgcagcg cttcagccaa ctgctccata ggttccgtgc gccgcgcaac
    24781 cagaacaaga ttggcattgc gctccgcgaa gagctttgca aactcgacgc cgagcccgct
    24841 cgatgccccg gtgaccaacg cccactttcc gtgcaagttc ttcattgtcc tctcccattt
    24901 ttcaaatttg gaactgaact gttttgcgtg ttgacgtgtc ccatccccgc tacgacggga
    24961 agcagcgcgc gaatgccagc aacaccttgg gcgagccgtg gatgcgaaac ttgcgcgtca
    25021 gcaaagccca cgcgaggttc gcttcctttc gcaggaagcg cagccaagtc tgactgtcgg
    25081 ccgtgatgcg gaaatccggt gtaccgctgt gcccgtcgga tactgcgagc ttcttgtcgc
    25141 gaatcacgac cgtggccttg acctcctcct ggccggtaaa tgtgaagtga tatgttgcgt
    25201 tcagcccctc cgatttgccg ctctggaaca cgaaattcag cccgcgcagg aacgcgcgga
    25261 tcgtcccctg tcccgccagc ccattcgaga ccctcttggt tttcttatga ggaaagcggc
    25321 gagccacgta gtcttcggca tcggagccag gaaccacgta aaccgtttca actttgtctt
    25381 gcagcggctt cacgacatcc gcgaggaatt gtttgcggtt ggtcagaaag ggcgcgatca
    25441 cttcctcgcc agcggggcag acggacaggc aatacgccgc cttgtagttc gcgccaaacg
    25501 agaggctctg ccacatcgaa actgactctg ctcccgaaac cttcctgcgg taatcaagcg
    25561 cgtctttact gtccgcgatc ttctcgaccc agtcgttgaa tccacccatg aagtcgcggt
    25621 agttgtgcgt atagcaagcg gaaaaattga agcttccgtc gctgccgatc gctccggtcg
    25681 ggcaggccgc gacgcacagc ttgcattcga ggcatgggtt gtaatcgatg ggatgcgaat
    25741 attcggacgc ttcggcatcc atcaacaccg tcccgagcag gatgaaattg ccgaatttcg
    25801 gatgaatcac attacgatgg attcccatgc gcccgaggcc tgcagcaacg gccacaggct
    25861 tatgggaaac gacccacatc tttgctcccc agcgatccgc ttccatgggg aagccggcgg
    25921 ctcccccatt gacggcgcgg acgccaattc gttccaaggc cgcgacaatt ttgcgggcca
    25981 cttcgttcgt ttcatccgtc gtgtgatgga attcgagatt ggcgacagag cgggcaggcg
    26041 tgcgaatgtt ttcgcggttc atgcgcaata cgaagctgac gagcgttttg gacttgggga
    26101 gtagccacag gacgtccgtt ttttggtcgg caatttccgg gcgatcaatc tcgacgaagc
    26161 cgacatcgtc cgcaccagct tcaaggcaca tttgacggag ccactcgcga tcgagcgtct
    26221 gtggccggac gacttctttg tttgccgttt ctctcgcctg aaactgcttt accgtaggat
    26281 gttctgcaag ttgcaccgtc gaaccctcct ggttatacat gtatatgcat ctataaactc
    26341 aaaaaaatct agccgccggc ggacgtggcc gtaaccttcg ctagaaggac tgccaattga
    26401 cccatagtgc cttcacccag cacgttctgc agacggttct gagcccgctt ccagtgcggc
    26461 actgaatgct gcaacttcgc ccgcccagcg tttgtcagtc ggatgatgcg aaatcgccgg
    26521 tcctcgccct ctttctcctg aatccagcct cgatttctca acagttccag catgcgcgtc
    26581 aaagtcgtcg aatccagcgc caacactttg cccagctttc cctgtgtgat ttcgcccgcc
    26641 cgcgcgagtg ccatcaggat cgtgaattgg gtgatttcga tctgctgcgg gcggagctcg
    26701 tggttgtata gccgcgtgac cgcccgtgcc gcccgccgca agtttgcgca ggtgcaaggc
    26761 aacgtcggca gccccgcgat gtattccgcg cttgtatttt gcgtgctcat acacatgtat
    26821 atacatctag cgtcgccgcc gtgtcaagtt gcttttttgg tccggcaaat ggcggtctga
    26881 agatggcgct acgcgggcgg cgcggagaat tcctgcgcgc cgatgacgtt aagaatttgg
    26941 gttgcggtcc gctggatata tattcgaggc tctcgctgag gagctccgca tccccgccgt
    27001 acaggccgat gtaatcttgc gcggcgctgc ccgtttggag gtcgattgcg ctgcaaacgg
    27061 gctcggaagc ttggtttgca gacccgcgcg tgaaacaaac gatcatccta gtcgcaatgt
    27121 cattatgcgg cttcctcaaa caacgtcact ctattgagtg cttcggagtg tgccgtgccg
    27181 cagtgcgcgt tctttaagct gagcgaacag aatcggtacc aagatcatga cggcaatggt
    27241 ggatgtaacc atgccgccga cgattggggc agcaatcggc ttcatcacat cggaaccgat
    27301 tcccgtttcc cacagaagcg gcccgagact cccgataacg gcgaacaccg tcattagctt
    27361 cggcctcagg cgcagaacgg ctccgtcgat ggttgccgct tcgatgtctt cgtgcttgag
    27421 cggaattccc aaagccaagc gtgcgtccag agcctcgtga aggtaaacga tcatcacgac
    27481 agctgtttca acagcgattc caaataaatc gatgtagccg actgccacgg cgacgctgaa
    27541 gttgtaacca agtatcacct gaaggagcag gcctccgatc aaagcgaaga aaaccggaaa
    27601 gaacagcatc gcggattccg ccacagagtg gaaagtcatg tagaggagca agaaaatgac
    27661 aaaaaatacc acgggcaaaa tcaattcaag tcgttttttg gcgcggagtt cgaactgata
    27721 ttcccctgac cattggtacg tgtaacctgc tggaagagcg agcttctgtc gaagcaggct
    27781 atcggctttt tggacgaatc ccccgtagtc gctcgtattc agatcaatgt aaatatatcc
    27841 agtcagtgcg ccatcctcat cgcggatcat tgccggtccg cgagagaacg agacgttggc
    27901 tatttcggaa atcgggattt gagcgccgga aggagtcgca atcagaactt gtcgtaattc
    27961 gtccagattg ccccgaaaat cgcgattgta gcgaacattg attggatacc gctcgcgtcc
    28021 ttcgatattt tcagcgatgt cttcgccgcc gataccagat tcaaccgcac gctgcacatc
    28081 cgcgacggtg agtccatagc gtgcagcgac ttcccgattc atggccacat tgatatagaa
    28141 gccttgcgaa acacgttccg cgaaaatgga ccgcgtttca ggaagcccgg aaagtaattg
    28201 ctggattttc gcgccaacct gctgaatgcc gtcaagagat ggcccttgaa tcttcattcc
    28261 taatggagtc tttattccgg tcagctccat atcgagacga ttttcaaccg gcatcgtcca
    28321 cgtgtttgat aaaccgggga attgcagctt cgcatccatt tgctggatga gtttctcgta
    28381 agtcatgccg gcaggccact gattgcgagg tttgagcatg atcgtcgtgt cgtacatatc
    28441 gaggggcgcg ttatccgtcg cgctgtcgga gcggcccacg gttccgaata cgctttcgac
    28501 ttcggggaac gttttgatga tccggtcctg ctcctgtaga aggtctgagg cttgcccgat
    28561 ggagattccg ggcagcgccg tcggcatgta cagggcggaa ccttcaaaga gcggtggcat
    28621 gaactggctg ccaatcctgc gtgccagcgg cacactaagg gcaagacaga tcagcgcaac
    28681 aatgatggtg cttttccgaa atcgaaggca gagccgcaaa aacggaagat aaatcgcttg
    28741 cgtaatccgg gagacaggat ttcgtgcttc agcgcggagg cggcctcgca cgaacagcgg
    28801 catgagaacg ggtaccagag tgatggcgag caccgaagaa aatgcaatcg ccagcgtctt
    28861 agtccatgcg agcggcttaa acatccggcc ttcctgcgct tcgagcaaga aaaccggcaa
    28921 gaaggagacc acgataataa tgagtgaaaa gaagagcgcg gggccgacct gctgcgcggc
    28981 cgcaagcagg atgctccgcc gttcggtttg gggaatgcgt acggcactcg gagaggcttc
    29041 tcggctcgca accgcagaat cgaatggcaa gttcttctga tcgggaggat gactctgccg
    29101 ctcagccaaa tggcgatatc cattttcgac catgacaatc gccgcatcta cgagcacacc
    29161 aattgcgagc gcaagtccgc ctagggacat gatgttgctc gaaacatgca ggtagtacat
    29221 cggaataaac gacgcgacga ccgcgatagg aatcgtgagg atcggaatta acgccgaacg
    29281 gaagtgccag agaaatatga tgatgaccaa gctgactaca atggcttctt cgatcaaatc
    29341 gcgctcaagc gtgtgaatcg aggcctggat caagccggag cggtcgtaac cagaaacgac
    29401 ttcgatgcca ggcggcaaag attttttaat ctgcgcgagt ttctgtttga cgccattgat
    29461 gactgtcaga gcattcatcc cgtagcgcat cactatgatt ccgcctacgg tctgcccttc
    29521 gccattccac tcggcgaccc cgcgccgaat atccggccca aacgagaccg ttcctaggtc
    29581 acgaatcaga accggcgtgc cgttcttcgt tgcgacggga acatcttcca agtcgttgag
    29641 cgaatgaaga tagccgcggc cgcgaatcat gtattccgcg ccgcccagct cgagcaatcg
    29701 cccaccgacc tcgtttgtgc tggcgcgcac gcgatcaatt acggtggaaa gcgggatgtt
    29761 gtaagcgcgg agcctgtcag ggtctaaatt gacctgatac tgccgcacga atccgccaat
    29821 gctggcgacc tccgcgactc cgggaacggt ttcgagttgg tatcgcagat accaatcttg
    29881 aaggctgcgc aaatccgcga ggctgtattt gtgtgtgtgg tcaacgagcg cgtactcgta
    29941 tacccagcct gcgccggttg catcggggcc gataacagga tggacggctg cgggcaagcg
    30001 gccctggatt tgctgcaaat attccaagac gcgcgagcgc gcccaataca gatcggttcc
    30061 gtcctcgaaa accacgaaaa cataggaatc accaaacatc gtctgcgctc gcacggcttt
    30121 cacctgagga gcggctagca tcgtggtcac aattggatag gtgacttgat cttcggtgat
    30181 gttgggcggc tctcctgccc agttggtgta gatgattact tccacatcgg aaatgtcggg
    30241 gagagcgtcg agcggaattc ttctcatggc ccagaggccg ccgagtacaa gcaaagcaac
    30301 ggctgtgaag acgaggaatg cattccgcgt gcaccaatga atcacccgcg cgatcacgct
    30361 acattcctcc cgcagcgtta acgctcagtt gcttgctgcc aatcgtttgc ccgttctttt
    30421 gggcgacgat ggtcacttgc caagtgccgc cgcttcccag ctggcccgtg ccttcgtaaa
    30481 ctccgcttcc tttatcggtg agagtcgcag tcgtgcgcat tgccgacata cccattcctg
    30541 gcatcgcagg cataaagaac gtcaccctga cttgtgcgcc caaaatcggc gctctacttc
    30601 tatttgtgag cttcactcgg aagatgtttg tacctttgtg gggaggattg ggttcggaac
    30661 tgaattcgat attcgattgc gacacattga ttgcggaggc cgcccccgct cctggaggtg
    30721 gcgggataaa cgatccgagc gcggcttgca gctggctctc ggaatcaatc aagaaattcg
    30781 cggaagtcac gatgcgttcg cccgcttgca accccttcag cacgatgaag ttgtcgccaa
    30841 ctcgcgcacc aagctgaacg tcgcgaggat caagatagcc atccccgcga tccacaaacg
    30901 caatgctgcg catgccggac tgaagaacgc cgcttgctgg aattaccagt tggcgtccca
    30961 ttggaatttt tagactgacg ctcacgaaca tgccgggttt gaggaggagt tcagggtttg
    31021 caaacacaat ccgcacattg gcagttcgcg tgttcatatc cacttgggga taaatcaaat
    31081 ccagatggcc actgaacgtg cgacccggat atgaatccac cgctaggctg gcgggatcac
    31141 ctttcctgat tcttccaagg tcgttctgaa aaatctgaac ctgcacccac actgtcgaga
    31201 ggtcagcgat ggtgtagaga cgcgtttccg gctgcacggt gagaccgggt aacgcattgc
    31261 gttcggttat gtaacccgaa atgggggaat cgatttcaag ttcctgctgg acttggtctg
    31321 tcgattcgag cctcgcgatt tctcgtcgag gaattcccca ttgcgtcaag cgctcaaggg
    31381 cggcgctgac aagcgatgct gcgccggaag ccactccggg aaccgtgcta tgcgccactt
    31441 gctgctgatt ctgctttgcc aaaatgtatt cgcgctccgt cgcaaccaga tcggggctgt
    31501 aaatcgtgaa cagcggctgt cctttatgga cgtactgata agtggcgtcg gcgaagactc
    31561 gctggatgta accggaaaaa cgcacttgca cataagcgag cttggtttca tctatagcga
    31621 cattaccggt cgtgcgaatt tcatcctcaa catatttccg ctgaacttga ccaagcgtca
    31681 cgccaatgct ctgcaatcgt tgtgcggaga tttgcaccgg gactaatcgt gtttcagggg
    31741 cagttgtgga cggcgctgga tttggagcag gggttcgctg gaccatgact gaggattttg
    31801 ggctctgcgc attaggtgtc ggatcaccgg ggtgatatcg aaaccagacg aaagcgatca
    31861 cggcaatgaa cacgacattg gctgctaacg cccagatgaa tccagcacga taggttttca
    31921 ttgctgtgct ccttcctggc gtagcgacag gccagtgagt tgctcaatac cggccagtgc
    31981 gtcctcgcga tcagcaaaag tctgccagca ctcctcgtcc aaatgcagga catcgaggaa
    32041 agagttcaag agggtctgaa aatcggagcg gttattctga taggaagcaa tcccggcttg
    32101 atattcggca cgcgcctgcg gcagaagtcc gtggttgtac atattcaata gctcggcatc
    32161 tttttgcgct atgtcgtatt cggttcgaag ctgaaacgcg acctgttgga attgtgcttc
    32221 cgcctcactg cgagagcggt ttaggtctgc ttgcgcctcg gctaattcgt ggtgaagttt
    32281 gctgcgatgg atgggaatat taacgccgaa agtcagcatg tagtaagcac ggtactgggt
    32341 gggatcggtg cgctgccaca tgtattggag gttgaaatcg ggatagaaat ccttgtgcgc
    32401 cagatcgact tgtagttttc ctttctgaac gagactctgc gccgccgtaa tttctggatt
    32461 ctgcgatttg gcggcattca gaagctcatc gaaggtgtac gtcagcgggg tttgcgatat
    32521 ctggatcgtc tcgatatctg gtgatgattg tgaacgatta agaagctgtt tcatctgcgc
    32581 ttctagcttt gctacttcga ggtgatgcat aatgatttcg cggagcagtt tggttttttc
    32641 aagctgcgct tgcagtaggt cttgctggtt ccccattccc gcgcgatatt gtgcgtcagc
    32701 ggcttgcgcg gcctcattga gcagttcgcc atcgctctga aggatcgcaa gcgtctgaga
    32761 caaataggcc aatcggaagt atgcggattt gacgttcgcc agcacctccc tacgcacgga
    32821 ctcgtactgt tgttgcgcga cagcgctatc ctgcttcgcg atctgcccct tgaggcgcaa
    32881 tttacctgga tacgggaagt cctgcgacgc accaagtccg atgtacgcaa agttgctgtt
    32941 cgtgtatccg gcgaacggac gcgggctgcc gacgctgaac tgttggactt ggacctgtgg
    33001 gtcgggcagc gcagacactt cagcgggcac ctgttgtgcc gcgatccacg cttggtgcgc
    33061 tgcctgaatt tgcggattgt tccgctgtgc ttccgtaagc aggtcgctca agcgcgcggg
    33121 cgtctgcgcg tccgccgcta caacgtgcac tggattgttc ggcgttactg gcgtctgccc
    33181 aaatacttcg gacgcctgca gcaagaaaag caagagcgcg gcaatcagac atggggcgaa
    33241 ggtaagcttg cgggtgcgca attgacggaa tggtgggaac atgttcgtgc tctccttcaa
    33301 gaaatctaag tgtgcgctcg tcgagagtcg cacaggcact cgcctcgata aaacaagggc
    33361 tcagagtgaa aaatggcaga gataaaacac cacgtgtgca gaacgcacac ggataaggcc
    33421 ggtctagatc agaagcgatg cgtgaagggt gtacagctcc ggcggagagt gttgctgcgc
    33481 gtctgtaatt ggcggaaatt ccaaaaatcg atgcaaaagc ttaacaatgg gcgggtattc
    33541 aaaaactcca aggctcggag cctgcaagga aacagattgt gcgggaatga tagtcggcga
    33601 tttgccggaa gccatgtcct tgcagcaccc atgactgtga ttcgcgggtg tgcatggcat
    33661 tgaagcgcag catttcatgc tctgctcgat tgctcctgcc gtaggcaggc aggatgcgaa
    33721 ctgcatcatg agtgcaagag ccgttgccgc gagtgctgta aagagccgct tcatggcgtc
    33781 cctcaaatct ataatccttg tacgcgacag tcaaccgatc gattgtgagg atgtcgagtt
    33841 gtgcctgctg tgttgctata agcgttcggc tctaacaatt gcgaggattt tacttcgaag
    33901 gaatccccta ctacaatccc actacttgat cgcgcggggg caatcatatg gcctgaaaag
    33961 tggacgcctg agcaagtggc tgcgggccgc catgaagagc agcggcgtca aggttactat
    34021 cgggaactgt atcttaggag gagcggcagc atacgcagat tcttcgactg gttaaggcgc
    34081 ctttggactc gaagggagaa cctttagcgc ggatcgacgg cagtcagcgg cagtcagtcg
    34141 tgtagcgtgg ttcaatgatt cggaaaaggg gaactgtcgc gctgatcatc gcatcgcacc
    34201 cgcgttggct cagtagccga aggcagcagg accgtctgtg ccaaataatc tcaaagcgtt
    34261 tcagaacggg atcgaaaaca aaagaccacg cctgagcgcg ccgtaagcac gtgctctcgc
    34321 tgtgccagaa atatgtcaaa acgctatgtg tttcggccct tgggcttagc tttcgagcga
    34381 aaagctgccc caagttatta caaaagtgaa cggaacgtca gaaggtgtgc agttgcctcg
    34441 gcggcttgca aagcaggtgc acggtctcgc agtcgttctg cacgaagagg gtagggcggc
    34501 atcgcgccga atgacggggt tcatccccaa gaattcagtc catcttcggt ccaattcaag
    34561 ggtagggtaa gggccggaag gtcccgcaag cgacaccaga agcggcactc agatttctaa
    34621 accgcattct tcgggaccag cagatcgtgg ttcaaatcca ctcgccccaa ccgatctttc
    34681 ttgaatcagc ggcttacaac tttagcaatt caagaaaaag cgcaatgagc gcctggtacc
    34741 aaacaggcgt tcttgcctca tctccctttg acgcgaacac tctttgtcct ttgagttcat
    34801 gttccgtggc cccgccaaga tgcaatcccg ccaatatgtt gattgcagga aatcattaga
    34861 gaaatgtcca ccacaacgag ttcgtgcgag gggaaggcgc ttcggtgaag acggaattcg
    34921 tggcacgggg acaagctgat gcccaagcct aagtttttag gctctgctgc aatttcggcg
    34981 gacctgaccg acaaaacggc cggcatcctc cgctccttga acccgcgaat gcgcagggag
    35041 cgacacgtat ccccgcgaaa gccgcctgga taaaatggcg cttgactctg gaccgtactc
    35101 tagggtgtag ccttccgaaa ggagtcaagc gatgcaaatt ggcgtcgttg caaaaaggct
    35161 cggcctcagc gttgatgcaa ttcgctttta cgagcgcaat gcattgctcc cgcggcctcc
    35221 cagaacggag ggggggttcc gtcagtacgg agaaaacgat gttgagacgc tggttttcat
    35281 tcgccgcgtg cagggactgg gattcaagct cagcgagatc cgcggccttt tgaatctgcg
    35341 cggaaaccgc ttgcggccat gcgcgccggt gcgcagccgt ttggaagaaa aacttgctga
    35401 cgtacagcgg aaacttgctg accttcagaa gctaaagcat gaactgcgat tggctctgcg
    35461 cagttgcgac aaagagctgc gcaaacagaa tgctctttgt ccgatcctaa gagaaaccaa
    35521 tagaaaacgg agggtgcgag gtggaaattg aggtcttgta cgtatcagag tgcccggctc
    35581 atcccgctgc gttgaaactc gtcaaagacg tgctggcagc gcaaggtgtg gcggcggaca
    35641 tccatgaagt gcttgttgca gacgaaaaaa tggctcatga gttgcaattc gttggttcac
    35701 cgacgattcg aatcaacggg cgagatgtag ccgaagaatc gcaagagcgc cagaactttt
    35761 cgctgagctg ccgtttgtac gcggggtcga aatatgtggg gcttccgcca gtagaagtaa
    35821 ttcaccgcgc tgtctccgaa gcccggcgag ggggaaaacc gtgaaacaag ccaaaggcat
    35881 ttcggcgtcg ataggagcga ttctcagtgt cctggcgacg gtcggttgtt gctttccgat
    35941 cggattcacg gccgcgttag gagcgggcgc tgcaagcgct ttcttcacta cactccgtcc
    36001 atttcttttg ggactgtcca ttgtgctaat cggcttggga ttctggcagc agcgtcgagc
    36061 caaacagtgt gcgctccggg gcagatggat tagcgaaata atgctttggg ccgccgtggt
    36121 tattgtcgtg ggaatgattc ttttccctca acaagtcgcg gggtttctcg cgggcgagtt
    36181 ctggcggtgg ccgaaatgaa aaagcgactc gtggttctcg ggttagtggt tttggccgcc
    36241 gtggtcgccg cgatcttctt gggtggacct gggactgtac cagcgggcca gaagccgtta
    36301 gcaacacttt catcggcgaa tctcggcgcg ttcgaacggg cctttaatgg gaatgcggat
    36361 atgcctcggc tggttttgct gctctctccc acatgaccgc tttgccggcg gggggcctcc
    36421 gcaaccgagc agattcttgc caagcatcct gaatggaaac tgcacgttct ggttgtctgg
    36481 gaaccgattc tcccgaccga ctggcgaccg ccgagcggga gcacactagg caggatttca
    36541 gacctccgag cgcagcaatt ctgggatcca caacatgaag tggcgaaggc attgagccga
    36601 atcgagcagc cggattcagg cgagaagttt cattgggatg aagcactctt gtatgcgccg
    36661 cattccgaat ggaaggaagc accacagcca ctattttggc agggaccggt gtacaaggtg
    36721 gttgcggggc ttgaagctgc ggtgcgtaat gtctggtgct gccaatccag acgaccggct
    36781 tgatgctggg cgatgcagtc atttccaaat tatgcctaaa cgagcatagc agaacttttg
    36841 gccaacgtcg gtgatctcac aaaagtctat cgtgcagcgg actgtgcaca atccaaagga
    36901 gcttcgactg tgcaccaatt gtgcaacaat tcacctgttt tcagcgcatt tgggttagct
    36961 tgagcgaaaa gcaaattccc cgatttgttg taaacgccgg cggttagaaa ttaccagcgc
    37021 ggttgttgga acagattcgt ccgctttgca agcagggttc gaagcccctt aggggtccgt
    37081 ggtcggcggg attgggcctc aaacgggtgc gcaccttttt agagttttca gactgataaa
    37141 ctgggtaacc ggactttgtg ggcattagat cgaatctttc cgacagagtc gggcagcttg
    37201 acctaggagc gttcgatctt gggcgtcgat tgcgctccta acggttcacg gaaaggatcg
    37261 atcggaatcg gcgcgtattc aacgtcgatg atctcaaggt gttccgtctt tgctggcgcg
    37321 cgcagttcca cgtggtcgcc ggggctcgcc ttcatcagtg cgatggcgag gggtgaacgc
    37381 caactgatgt agccgcggct aaggtccacc tcgtcgatgc ctacgatcga gacgacgtgc
    37441 tcgaggccgg aggcgtcctt gtaggtcacg gttgcgccga agaaaacgcg cgtagcgact
    37501 gagtccggcc gccgagcggc gggatcgacg accacggccg cgtcaatgcg tttcgtgaga
    37561 aagcgaatcc gcgaatcgat ctggcctaac cgtcgtttgc cgtagagata gtcggcgttt
    37621 tcactgcggt ccccgttact agcagcccac gctacgacct ccaccactgc gggccgctcg
    37681 cgactcagca ggaacacatg ctcgtctttc agtcgctgca acccggcggg cgtgatgtag
    37741 tttttgagtg ccgggcccgg gggctcgcgc accggccctc ggagcggccg cttttctcgc
    37801 atttgccaat ctgtcatggg attccgtgta cttgtggttg accgaatctc ggaaaattcg
    37861 cgcctcggtc cagataattc ctcatgcggt gaacgtgacg tacccagcct agatgtcgaa
    37921 tgatatatcg gtctttcctg tggcactctt agaaaatgct gtaaccggcc cgattacata
    37981 tcaaggcgaa gacgtgcgcg gttcgcctac ccacgctaac aattctgcga atgcgatcgt
    38041 gagagaagct atacctcccg attaacgggc aaaccggaag taatcgaaca tcggcgcggg
    38101 tggcggggtg cgcggggtaa caattctccg ttttgttacc tctgcactca gtatccctcg
    38161 agcagctgaa gtatcattca tcgaaattaa cgtttgaagt atcgtgctac aatccgcggc
    38221 cattgtggcc agcgagaaat ccgtaacggt ccccgtaaaa cctcaatttt gggaatttta
    38281 tcggacaaat tttaagctct tcgtacgccg tcgttggcaa cggacctgaa gacatagcgc
    38341 tcgctcgggc gattacgcga gcaaaggtgc cgaacgcgaa aatcggtgaa atgcctcgta
    38401 gcaattgcgg agtacaactc tgaaaccaag ggcactgctt tctccataga gcttggagag
    38461 ctttactaca accgcatgct ccgatttgtg ggtatcaagc agtgtgaggc gcaactaagt
    38521 aaagtgctag acagcaataa cgctgccaac gatcaggcgg ttttggtcag cccgtaggcc
    38581 gagatcctgg ggacagcagg ggactttgtc cgccctggtt ttccgaaact ggaaagccag
    38641 ggcttacttt tcgtcgtccg attcggatct tgcatccgtc cgcgccacgt cgctcgaccc
    38701 cgtgcgtatc atttcgtcgt tttgttacta cttcgagcgc ggatcctcgc cgcttccgag
    38761 ccaatggggc ctttttcccg tctcctcgaa gttcgtcacg ccatctgcta tcctgccgcg
    38821 catgaacagg gcgcgagtta cggtagctct ctgccttctg ctttttttcc caccaatcac
    38881 gagtaggacg tcaggtgcac agggacaggt gagcatgacc ctcgaacaag tccattccat
    38941 tgtccaaaaa tacgccgagc agtggaaccg ccagaaccca aaatatcccc tgtgggtggc
    39001 tctcgcgagc cccggtgatc ctgtcaatat tccgggcgcg acatttggca cgtcggacaa
    39061 cccacaagcc ggaatggaac taccggtctg tgcacacgtg attgtgataa acaaatcgat
    39121 ggtgcccgag agcatcctga cgacattcac ccacgagtat ggacatctca atatcgactg
    39181 gcccaccccg gtgattttga cgatgttgct tcagaaattg ccgccattaa gagctcgctg
    39241 acgttgcttc cgaaagaagg tttagaaagt ctagcgtttc gcgaggccag agccatcaga
    39301 gagatggcaa accgcgaccc ctaccgaagc gcggtccagc ggctcgccga tgatccgatc
    39361 tggataaaat attctcgata gcccaaagtt catagctgaa cggcaaaacc ttgcgttttt
    39421 cgcagccaga ctgaatcgat ggccctgacc gcggaccgcc aaaagtttct ttcgggctga
    39481 acatatttcc tctttttcca cctcatttgt ggtataaaaa caggaagaag ttcagaggga
    39541 gcgaatgaaa acaaacgtgt tctcgaaacc tgaagaatcc agggaccgtc ttcgacagct
    39601 tggtctcgat gcattggtct tcgtgcaggc tattcagcgc ggacaggcga ttgccgctgg
    39661 gtacactcca aaccatccgg cgatctgccg cggaatttcc cagtggggag aggttgtcgc
    39721 tgcaatccgt gagcagctct taccgcgggc atggactcgc atggacgaag acaattttcc
    39781 cctcaccgtc aatccccctc ggaacatcgc gatatcggtc tcaactggag acgcgagcac
    39841 ggggatcgtc gacgcgaccc catcgacgcg atctacaaaa ggcccacgca ccgcgaacgc
    39901 ggtaatcgtg aatgcgctgc agctcaacct cttcggcaac gttccgctcc ggccggaaga
    39961 attgaagtgc tcgaacggcg tgatgacctg gattttccta ttccaccgcg acgtagccgc
    40021 gcaagagacg cggtttgaac tttcacggcc ggtcaaaatg gattccgacg gccacgttga
    40081 tgtttgggtc gaaaggataa tcttccccgc cttgccgttc ggaacggacg gacttcttgt
    40141 gcctgcagat cttccggtgt cgccggaaat cattcctgag gtcaagcgca aacgtgcctg
    40201 aggctgctgg gatgttcaat ccgaatcgcc tctcggttgc tcgaaagagg cagggcttaa
    40261 ccaagaccga ccttgcgaag cgcgcgaggg tcgatctccg atctgtcact gcgtacgagg
    40321 ctggcgtcta cccgccgaaa ttggaaaccg cggagagcct ggccgcggcg accaagttcc
    40381 ccctcgagtt tctgtacggc gacgacctcg atgagcctca accggacact gccagttttc
    40441 gggcgatgtc gaaaatgaca gcaacgcagc gcgacatggc gcttagccaa ggaacgatcg
    40501 ctttgcacct gagcagatgg ctggaaactc gctttgaact tcccgagctc gatctcccgg
    40561 acctcagccg cgaagattct ccggaagccg cggcggactc gttgcgtcgt cactggggca
    40621 tcggtgaatt gccagtcaga aacatgattc atctactcga agcgaaggga gtgcgaattt
    40681 tctctttggc gatcaatgca cgcgaggtcg acgcgttctc actctggaaa gacatcacgc
    40741 ctttcgtttt tctcaatggg aacaaatcgt cagagcacag tcgttacgat gcggcacacg
    40801 aactcggcca cttggtgctg caccgtcacg gaacgccgca aggccgagac tccgaacgtg
    40861 aggcagacag attcgcctcg acattcttga tgccgacctc cagtatcatc gcgcatgcca
    40921 tgcggaatcc ttcactgccg gatctgatca agctcaaagt gatttggatc gtatctgtgg
    40981 ccgcgttgaa tcgccgactg tacagcgcgg ggatgattag cgactggcag tatcggacgc
    41041 tgtgtatcga aatcgccaaa aggggatatc gcaagcacga accccgtgag gccccgcggg
    41101 aaatgtccca catcttgcct tccgtttttg gtgctcttta tgaagacgga atcggcaggg
    41161 cggatgtggc gaaggcactt aaaattccgc gctcggagct agagcaactc atgttcggtc
    41221 tcacgatgac cagcatcgat ggcgggcgga atcagaccgg gtcaccggtg aaggcgaagc
    41281 tgacggtcgt cgagtaaaat ccagttctaa aattccccgc tgccgctgcg cgcgggtcct
    41341 tcccccgcgc caccctccac gggtgacggc ccggccgaga ggtgaccagc gcattccccc
    41401 ccaaagcgga cttgacttta ctttcgcaga attgctttgc ggacgttttg agcgtctgga
    41461 cagaacgtat ggagcgatag cgatcgcgag agcgcattcc gaaaaaccga aacgaagtct
    41521 gtccagtgcg gtcttacgca gaacctaaga cgcgcgcgcc gtcacccgcg gctgacgcgg
    41581 cgcgggaagg accccaaggt ccttcttaga ggtcagaact cggagcggtg atttcgaggt
    41641 ctgtcgcgcg actcgctctt ggcgccagtc aggtacaagc ttcccgccct cgttcagaac
    41701 catctcgatg ttcggtagaa ccctttttca tataccgctc tacgccactc cggacggccc
    41761 ggccagcctt cctcgggtcc acggcgaagg ctttgggggc gattttcctg gcgactcgtg
    41821 tccacgtatg cccttcagca cgaagcgtga ttgcaagctg ccattttcgc tcgtccgcgg
    41881 gtctaccgcc tttgagtttt cgccagacat ctttcatccg agcgctacgc cgggcccgct
    41941 cctcgggaga ggccagggtt ttccgcaggg tcgcaacacg tcgcgcgttg atctcaggat
    42001 ctgagaggtt tttcttcata gacgcgatcc tggccgcgcg tttctctggc gcccatccct
    42061 ttttgatcgc cgccgcgaca gcagctttca cttcggggcg ggcagccgcc tctttgatgg
    42121 ccgcgatcat cttttccccg gtgagtggat tagcccattg cttcttagag aactcgctta
    42181 aacgcgcctt agcctcgggg gtggaccatc tcgcctttgc cttagcggcc agtcgcgcgc
    42241 ggcgctgcgg atctgatgcg gctttcctca tggcggcgat ccgctcggca cgcttctgcg
    42301 ggtcggccca ggctttctta ttcgccgcaa ggaaggcggc tcttgcttcg ggcttcctat
    42361 gtttgaacgc ggcaagccat ttggccctcg taatgggatt agcccattgc ttcttagtgg
    42421 actcgcttag ccgggctctc gcttcgggat ttgccattcg tttgctcatg gcttcgctga
    42481 ttagcgcacg cagagaagcg ttcattgtat ggatctctcc tgactcgcct tttttcgccc
    42541 ccttcccttc cggtaccgtc gtcccttgta gacgacgacg cgggcgacat cggagtcagt
    42601 ccagtagcga acggtcgcca acccgacctg cgcggtcctg ggacctgaaa acttgccggt
    42661 ggccagccag cgttcgagtg tcgcccgcga tatgcctgcc tttactgcta cctgtttcgt
    42721 tgagtagtgg ttcatgcgcg acagcttgtc tcacattaac aaccgatggg tcaattgcca
    42781 tgaaaggttt cgtggccact ttttacaagc tcttatggcc atgaaacctt tcgtggcctt
    42841 ttctacagtt cctatgggca cgaatccttc cctggccact ttttgcagct cttatggaca
    42901 cgaaagcttt cgtgtccttg ctgagcgtac acgattacgc catgttcatg cccgcaaacc
    42961 cgtgcagaac ccatcgagag gacctcaaag tgaatgccgc cagcgatcag ccatcgcgat
    43021 cgtcagacaa tcctctttgc aggcgggcag tagtacaggt acactccgca cccaaagggc
    43081 agccgagtca gcgagccagg agaacggctc gatggcgagg cgacggtttc aaaccgggtg
    43141 gatccgtgtt cgagggaaaa accgcccggt gtttgagggt cgatggcgtg aggacgtgat
    43201 ccagccagac ggaacgatca ggcgtgtgcc gcgctcgatg gtgctcggaa cggtctccga
    43261 gctcaaaacc aaacgcaacg cacaacggtt gctcgagcca atcctggcgc gtattaactc
    43321 cttcgactat cggccgagca agttcaccac aatcgagagg ttcgcggaca cgtgggaaac
    43381 ccaggtgctc atacatcaga agccttcgag cgttaaagcg gcgaagtctc acctgcggag
    43441 tcacataaga aagcaactcg gcaaagtgct tcttcacgaa ttcaccacgc aaatgcagca
    43501 agcgttcgtg acgctgctcg cgcagaagcg cgtttcccga aagacgattc aaaacgtact
    43561 cgggacgctc gcctcaatgg taaaaaccgc gaaggcctgg ggttactcaa tccagccgat
    43621 cgtaaccggc gagctccatc tgccggccga ttccgttcgg gaaccagccc gttttttcac
    43681 cggcgaggaa gcgaagaaca tcatcactat cgcgcctcag ccctaccggg cgatgttcgc
    43741 gatcgctgga atggctggat tgcgggttgg agagatcgtc ggcctacaga aggccgatct
    43801 cgatttcgac acccgtcgga tcttcgtgcg gcgatctgct tggtatgggc gagtccagac
    43861 ggcgaaaagt ccagccagcc agaagccggt tgcgatgtca aaagatctgg tgccactgct
    43921 cgaagaatat ctcgaaacgt ggcgcccgaa tccggaaggc tttctgtttt tgaatcgcaa
    43981 cggacgccct tatgccgcga acaaagtcgt cgagtatggg ctctggcccg tgctcgataa
    44041 actaaaaatc ccgcgcgccg ggatgcatgc tttccggcac gcacacaata gcttgctggg
    44101 gcacgtggga gccaatccaa tcgtgatgaa aacgcagatg agacactcag atgcacgtgt
    44161 cacgctggat ttctactccc acatcattgg cgacgaccag cgcgcagccg tggacaaagt
    44221 gggcgaaatt ctgcgcccaa atgagcccaa atctgggagt gacggtgagt atattcagta
    44281 acttgcgggt tcgcggacgt gggttcgatt cccatcgccc gctccataaa cttccttggt
    44341 tgcgttgcct gattcctcga aggcgagtgg ggacgcacta cggccgcaag ggtccttgcg
    44401 cctgaatttg cgtaacggaa ctgcctaacc acggttgtgg tatctctagc aggccgactt
    44461 tatacaattt ttttacaaag tccggggcgc cggaccgcgc cgtcctatcg gccgagacgc
    44521 tttagtcgcg ggtcgagcag gggctcctgc aagccggcaa tttcgcgggc tggtgccacc
    44581 ttagccttct tccgccactt caggaaatta tctgcatagg tgtaaaaaac cggggtgtaa
    44641 taaagggtaa cgaactgcgc gaagatcagg ccgccaacga cgcagaggcc caacgggcgt
    44701 cgcgcacctg catcagcgcc aaagccgatg gcgatgggca gcgtgcccat aattgccgcg
    44761 gcggtggtca tcataatcgg ccggaaacga atcatcgagc cctgatagac ggactcggca
    44821 ggcgagattt cgccttggcg ctcgaggtcg agcgcgaagt caaccatcat gatggcgttt
    44881 ttcttcacga ttccgatcag catgatcagg ccaacaaaac catagatgtc gagctgcatg
    44941 cggaagagcg aaagggtgag caagccgccg aaggcagcag caggaaggcc ggagagaatc
    45001 gtgacgggat gaacatagct ttcatagaga atgccgagca cgagatagat cacgagaacg
    45061 gccataatga gcaggatagg tagactggtg agcgactctt ggaaggcttg ggcgctgcct
    45121 tgaaaactcg tgcttatgga gatgggcagc atggaactcg cgagtttctg tacttcggca
    45181 acggcttggc ctagggagac tccaggagcg agactaaacg aaatggtgac ggagggaagt
    45241 tggccagtgt ggttgacggt gagcggaccc acgttggtag taaatttcga gacggcgctg
    45301 aggggaacca gctgccccga ggtcgaatgg atgtagagat cggaaagcat gttgggatcc
    45361 gcctggaagc gctgttgcag gtcgagaaca acccaatatt cattgttcga agtatagatg
    45421 ggcgagacca accgctggcc ataagcacta taaagagcgc cctcgacctg ctgcggcgtc
    45481 actcccaagg ccgaggcttt gtctcgatcg acaacaacat tggcctgagg attcttggct
    45541 tgcaggtcgc tggtgacgcc gacgaggtca ggaagatcct tcaatttttg taccaactct
    45601 tgggcgtggt cgtaaagagt gccagtctcg ggactggaaa gagtgaattg gtagaggctc
    45661 ttgctctgcc ggccgccgat gcggatggcg ggtggatttt gaagaaaaac ctctatgcca
    45721 gggactcttt ttagcttcgg ccgaagctcc tgcatgatgt cgtcgatcgt catgtggtgc
    45781 tcgaaaagcg ggcgaatgag acggacgagc gagtcgagaa cgggcgtgtg tccgtagctg
    45841 gtgaccaggc gatcgtaggc ggggctgtca gtccaaggac gatcgggaag atcccgcagg
    45901 tgcaagaata tgttgccgtt attcagtccg ttgctgctac tgtcgctgac tgaggagtgg
    45961 aactgcacta cgttgggatc cgcctggaaa atgcgattga tcgcttcctg atgagcaacc
    46021 atagcatcga aagagatgcc ctgattggct tcggtggaaa cctgaatttg ggagagatcc
    46081 tcctcgggca aaaaaccctt gggaataatc cagaattccc aaatggtgcc caccaaaatg
    46141 gccgcgccga caagcatgat gaagacccgg tgccgcagcg accagcgcaa gctaactccg
    46201 taccagcgta acgatccgtc gagtacgcct tccagtttgc gatagagcca gccatgttgc
    46261 ttttcgtgct cggcgcgaag atatcggctg cacaccatcg gcgtgagcgt aagcgagacg
    46321 aagccggaaa cgagcaccgc ggagataatg acgacggaga actcgtggag caggcggcca
    46381 aaaacgccag ccatgaaaaa gacaggcaga aagaccgccg cgagcgaaag cgtcatcgag
    46441 agaatggtga atccgacttc ctgggagccg tccagcgaag cttccaccgg atctttgccc
    46501 atctccatgt ggcgcacgat gttttcgagc atgacgatgg cgtcgtccac gacgaagccg
    46561 acggataagg taagggcgag cagggataaa ttgtcgatgg tatagccgag cagatacatc
    46621 accgcgaatg tgcccacgat cgccatgggc agtgcgaggc tcggaatgac ggttgcggaa
    46681 gcattgcgta gaaacaggaa aatgacaaga acgacgagga tgacagcgag gagaagtgtg
    46741 aacttcacat cgttgactga ctgacgaatg gggacggacc ggtcgtattc gattgagagg
    46801 ttgatcgatg gaggtatcag ggtaaagaag tgcggaataa gtttgcggat gcggttaacg
    46861 atatcgatgg tgttcgttcc aggctgccgc tgaacgccga gcaggatacc cgggatgctg
    46921 ttgatccagt tggcgacata ccggtcttgc acgccatcgg taacggatgc gatttgatcg
    46981 agccgcaccg gggcgccgtt ccggtaagcg acgatgaggg gacgatacgc agcggcgtca
    47041 ttaagctggc cgttggattg taggttatag gccttatgcg cgccgaagag cgtacccatg
    47101 ggcatattga cattggcgct ttgaatggcc tgttccactt cgtcgattcc gatctggcgg
    47161 ctggccatgg catcggggtc gagacccacg cgaaccgcat aagtggcctg gccgttaacg
    47221 ttgacttgag caacgccagg gatcatggaa atctgcgtgg cgattagatt ttcagcatat
    47281 ttatcgactt cgtagagcgg caaagtaggc gaggtgacgg cgagcgagaa gatggactga
    47341 tcggcgggat tgaccttatg aaacgtagga ggaaaaggca tattcgccgg aagctgattg
    47401 gcggcggcgg aaatggcggt ttggacatcg accgcggcgg aattgatatc gcggctaagt
    47461 gaaaattgaa gcgtaatttg acttctgccc agcgaactgg tggaagtcat cgaatccaag
    47521 cccgcgatgg tggtgaactg cttctcgagc ggtgtggcca ccgaggaagc catggtgtcg
    47581 gggctggccc cgggaaggcc ggcggagact tggatcgtgg ggaaatcgat gttaggcaga
    47641 tcgctgacag ccaagtcgcg gtaggcgaga atgccaaagc caaggatggc cagcatgacc
    47701 aggctggtca tcacccggcg gtgaatgaat attttgctga ggttcatcga attccaaact
    47761 ttcagattgc ttttgtgaaa tacaccttcg tacccggcac caggctcccc tgcccgtcgg
    47821 taaccacagt ctccccgacg gccagccctt tcaaaatttc agcctgattt tccacggttt
    47881 gccccgccgt cacttgccgc acatcgaccg tcatgtcggg tttcacgatg aaaacgaatt
    47941 gcccttgctg gcccgactgc acggcttgag atggcactac catgacgttc tcttgctcgc
    48001 caaggcgaag gagaacgtta gagtattggc cgggccagag gtggtggtcg gtattggcaa
    48061 acgtccccat caactgaatc gtgccggtgt ttgcatcgac tgtgttgttg acgaacgaaa
    48121 gaaatccctt ttcgggaacc gtgctgtcgg gcggcgtcgc ctgggcggct agcctcgatc
    48181 tctccatcgc ccgcttaatc gggctcagat actgctgcgg cacagaaaac gtcacataca
    48241 gcggtgacac ttgattgatt accacaagcg gtggcgtacc gttagccgcc acggtagcgc
    48301 ctggataaac gagctgcgca cccgtaaccc cgctgatggg ggccttgatt gagcaatagg
    48361 aaagctgaat tttggcggtc tcgacggctg cttcatcggc agcgacagtg gcacccgcgg
    48421 acgccgacgt cgcctcagac tgatcgtact gctgtttgga tacgacgcct tccttgtaaa
    48481 gctggtcata gcgttgcatg tccgctcgcc ccagttgggc ctgcgccttg tcccgggcga
    48541 gcgccgcttg agcctgtgaa agcgccgcga ggaaaggagc cttgtctaga ctcaccagca
    48601 agtcgccttc gcgaacaaac tgacctggcc gatagttcac ttctttcacg attccggcag
    48661 tctgcgatgc gactgagacg gtcttgaacg cttgcccggt accaatggca tgcaactcga
    48721 tcgggataga cttgcgctcg actttggcgg tgaccaccgg cacagcggat gtgctctgcg
    48781 gtctcgtaat tgcgccgctg gcatcggagc cctgcttggt tcctggacta tccgtgcagc
    48841 cgacaagaca aagcccaaac aatagcaaaa tcatcgctcg atatttgagt acgccaatca
    48901 ttttttcccg agccaaactg atttggctgg caagcttcgc cgcccgaacc gcccaaacac
    48961 atactcgcgc cgcggaaacg atgaacctgg ttcgacgaag attctagcaa agcactgcat
    49021 cccgtcttcc gcctttattt gacggtcaac gacggcattg cgttccgcgg gaataacatc
    49081 aagtgacgaa gctggattgc gcgccgttta atgctcgata ggatttcaac ggttaaacgt
    49141 cacaccgcct ggagaggcga tttctaggcc gtcgctctca attggaccct caagccactg
    49201 gatggcctga gcgatcgccg cacatctgag aaacctctgg cagtacagag cagcaacgga
    49261 gctggcaccg gagttcgagc aagggtagat tgtgccgccc gaacgcccgg catcccttta
    49321 gaatctgaag ttctgtgtgg atctacgggc ccaatcgggc atgccgacgc ctgggcccga
    49381 gagggcataa ttcgactgga cacttcaaac ggcgagggtt ccaatcctcg ccagtctggt
    49441 cacttcttat tggcaggcag cagcgccgtc cacggtacat tcggtaccac gtggccgctc
    49501 ccatcgatca gtctgatatt cgccgtttgc atgagtgagt tgacgccaac tatttcaaac
    49561 acaccctcgt gctctgcgct acggacgcgt tcgccattct ttggagcaat cgctttcgtt
    49621 gcagtggcca tcttcccctc c
//
LOCUS       DATNDT010000142         4706 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2908561, whole genome shotgun sequence.
ACCESSION   DATNDT010000142 DATNDT010000000
VERSION     DATNDT010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2908561"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1581)
                     /locus_tag="VN788_04665"
     CDS             complement(<1..1581)
                     /locus_tag="VN788_04665"
                     /inference="COORDINATES: protein motif:HMM:NF012931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HXU19856.1"
                     /translation="MKTLSLLFVVISAFSFAHSQQTPNAPQTQLNLMPWPANIQTGSG
                     QLVINSDFTIAISGSGDARVRGAAERFVHRLSQETGIPLRFTPAKSGAATLAIRAEHN
                     GKKIQELGEDESYVLDVNQAGAKLTAPNPLGVIHGLQTLLQLAAPGPQGFAFPAVHIE
                     DAPRFPWRGLLLDAGRHWMPVPVVERTLDGLEAVKMNVLHFHLSEYQGFRVESKKYPK
                     LQELGSNGKYYTQDEIKDLIAYARERGIRIVPEFDMPGHSQSWFVGYPELASGPGPYQ
                     IEKRWGVFDPAMDPTRESTYKFLDKFIGEMAHLFPDEFFHLGGDEVNGKQWDANPKIQ
                     AFMKSHELNSNADLQTYFNQHVVKIIEKYKKTPIGWDEVLNPGLPKDVVVQSWRGQES
                     EATAVRNGHRALLSNGYYLDLAEPASKHYMVDPLSGDAATLSPEQQKMVLGGEACMWS
                     EMVTAENVDSRIWPRNAAIAERLWSPGEVNDVASMYLRLEVISDWLDSVGLTHHSALR
                     EMRERLAPGRVDPLSVLAD"
     gene            complement(1595..2017)
                     /locus_tag="VN788_04670"
     CDS             complement(1595..2017)
                     /locus_tag="VN788_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19857.1"
                     /translation="MSHFGLNENHLRRLATSMSMVDAAAARVLDLLDEKTTPKSMTLM
                     EGASVTSSDRERIRELVARLQPLAIEFARKYELAKLSKDLRRTIAAEVSQIWTILENS
                     HAQNLRGMGPLSLGVAQQLDRDLEVMLNLIKEVIQTLR"
     gene            complement(2014..2760)
                     /locus_tag="VN788_04675"
     CDS             complement(2014..2760)
                     /locus_tag="VN788_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU19858.1"
                     /translation="MQPETLDRNGTIFGREAELTRLRTALAARRSFLFHGPRGAGKTL
                     LMTSLVREDSKLLYCSGARSLEACMRTIGRLLANNGNRMAAGKLDTRKAISAVSLNGI
                     LRAALAVSAYTIVLDHCGFTSQSFVSGLSEIVVPSATPIVAIARSPHMEELGYIRKLF
                     PFRSDNVELTNFAPATANNFVACAIATAQLHATNLPDFRERLLNASAGNPGAILTMIQ
                     MARQPKYRSGEQILFSPLYVDFRLRSEMLA"
     gene            complement(2750..4471)
                     /locus_tag="VN788_04680"
     CDS             complement(2750..4471)
                     /locus_tag="VN788_04680"
                     /inference="COORDINATES: protein motif:HMM:NF012570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HXU19859.1"
                     /translation="MRSEPRAERDRVLSLHRLAEIAAEFGADRIGSDALTLAERVSEG
                     RYFVACVGQFKRGKSTLLDALIGQRLLPAGVLPVTAIPTIIRYGNELRARVRNSSREW
                     HEIAPESIVDYVSEERNPENIKRVTAVEVFVCNPRLRSGMCLVDTPGLGSVYSGNTEE
                     TKTFLPHIDAAIVVIGADPPLSATELELVKHASKHVKDMLFVLNKADRVSTSEVTAAG
                     EFARRILERDLGKPIGHIYQVSALEELEGKRGRDWNGFVAALELLNRNSGRTLVEESL
                     HRGTSRIAGQVRQLVVDERGALLRPREESQRRIDDLNRLAVSVERSLKDLNPLFAAEQ
                     QRLSRIFAERSTRFIQSVAPAVHAELESFLRHTARGSGVTFRRAALAEAQRIVASQIE
                     PWLTQEQEFGEKEYSGSVARFAAMALDFIHRAAESGVTDVGSVSDMFSMPERFREKSR
                     FQFYRMIHIARPASPLLMITDLLLGLLNFYGPIERDAHKFLDKLLEHNASRVQNDVAE
                     RAAQSRIKLELEIQGLLRELAAAAQRSFNRASEVQQHGEEAVQAALDRLRALGVEIQE
                     IASSDAA"
     gene            complement(4449..>4706)
                     /locus_tag="VN788_04685"
     CDS             complement(4449..>4706)
                     /locus_tag="VN788_04685"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HXU19860.1"
                     /translation="TCRKLDIAGATVYRGIGGYGASTRIRRERHLLSFSSDAPITVII
                     IDTEEKIDKLIPALEPMLKEGLIVVSDVEVIKYVHEERAPR"
BASE COUNT          972 a         1362 c         1347 g         1025 t
ORIGIN      
        1 gtcggcgagc acgctcaggg gatctactcg acccggcgcg agccgttcgc gcatttcccg
       61 cagcgccgag tgatgtgtca ggcccaccga atcgagccag tcgcttatca cctcgagtct
      121 caggtacatc gaagctacat cattgacctc cccaggggac caaagccgct cggcaatagc
      181 ggcattgcgc ggccagatgc gcgagtccac gttttctgcc gtaaccatct ctgaccacat
      241 gcaggcctca ccgccaagaa ccatcttctg ctgttctgga gacaacgttg ccgcgtctcc
      301 gctcagcgga tcgaccatgt aatgtttcga cgcgggttcg gcaaggtcca ggtagtagcc
      361 gtttgagagc agggcccgat gcccatttcg cactgccgtg gcctcggatt cctgcccgcg
      421 ccaggattgc accactacgt ctttcggcag ccccgggttg aggacctcat cccagcctat
      481 cggcgtcttc ttgtacttct cgatgatttt caccacgtgc tgattgaaat aggtctgaag
      541 atcagcattg gagttgagct cgtgcgattt catgaacgcc tgaatcttcg gattggcgtc
      601 ccactgcttg ccgttcacct catctcctcc gaggtggaag aactcgtcgg gaaaaagatg
      661 cgccatttca ccaatgaatt tgtcgagaaa cttgtaagtc gattctcggg tgggatccat
      721 tgccggatcg aagactcccc agcgcttctc gatttggtaa gggccagggc cggaagccag
      781 ctcgggataa ccaacgaacc agctctggct atgtccgggc atatcgaatt ccggcactat
      841 gcgaatgccg cgctcgcgcg cgtaggcgat gagatcttta atttcgtcct gcgtgtagta
      901 cttgccattg gaaccgagtt cctgcagttt cggatacttc ttactctcca cccgaaatcc
      961 ctgatattcg ctgagatgaa agtgcagcac gttcatcttc acggcttcca gtccgtcaag
     1021 cgtgcgctct acaaccggga ccggcatcca atgacgtccg gcatccagca gcagaccccg
     1081 ccaggggaag cgcggtgcat cttcgatgtg gactgcggga aaagcgaatc cttgcggccc
     1141 cggagcagcc aattgcagca gcgtctgcaa accgtgaatt acgcccaggg gattcggcgc
     1201 ggtgagcttt gcgcccgcct ggttcacatc aaggacgtag gattcgtctt cgccgagttc
     1261 ttgaattttt ttcccattgt gttcggcgcg gatggcgagg gtggcagccc cggattttgc
     1321 cggcgtgaag cgcaacggaa tgccagtttc ctgggacaag cggtgcacaa atcgctccgc
     1381 cgcaccgcgc acccgcgcgt caccggaacc ggagatcgct attgtgaagt ccgaattgat
     1441 gactaattgg ccggagccgg tctgaatgtt tgccggccag ggcatcaggt tcaattgtgt
     1501 ttgtggcgca ttcggagttt gttgtgaatg cgcaaatgag aatgcagaaa tcaccacgaa
     1561 gagcagcgat agagtcttca tttcaggttc aactctatcg taacgtctgt atcacctctt
     1621 tgatcaggtt gagcatcact tccagatcgc gatcgagctg ctgagcaaca cccaaactta
     1681 gcggtcccat gccgcgtaag ttttgagcat ggctgttttc caggatcgtc cagatctggg
     1741 aaacctctgc ggcgatggtg cgacgcaaat ccttcgagag cttcgcaagt tcgtacttcc
     1801 tggcaaactc aatcgctagt ggttgcaggc gtgctaccag ctcccgaatg cgctcccggt
     1861 cggagctggt gacggaagcg ccttccatca gcgtcatgct ctttggcgtg gtcttctcgt
     1921 ccaataaatc gaggacgcga gcggccgcag cgtccaccat cgacattgat gtcgccaggc
     1981 ggcgaagatg gttttcgttc agcccaaaat ggctcacgcg agcatctccg agcgcagtct
     2041 gaaatccaca taaagcggac tgaaaagaat ttgttcgccc gaacgatatt tgggttgtct
     2101 ggccatctga atcatggtca aaatagctcc cgggtttccc gcgctcgcat tcagcagccg
     2161 ttcccggaaa tcaggaagat tcgtggcgtg caactgcgcc gttgcgatcg cgcaggcgac
     2221 gaaattgttg gcggtggcag gagcgaaatt ggtcagctca acgttgtctg aacggaaagg
     2281 aaagagtttc cgaatgtaac cgagctcctc catgtgcgga gagcgggcga tagcgacaat
     2341 cggcgtggcg ctcgggacga caatctcgga aagcccagag acaaagctct gcgaagtaaa
     2401 accgcagtgg tcgaggacta ttgtgtaggc gcttacagcc aatgcagccc gcaaaatgcc
     2461 gttcagcgat accgcggata tcgccttacg cgtatcgagc ttacctgctg ccatccggtt
     2521 gccgttgttg gcgagaagcc ggcctatcgt tcgcatgcag gcttcgagac tcctggcgcc
     2581 agagcagtaa agaagcttcg aatcttcacg caccaagctc gtcatcagca gcgtcttacc
     2641 tgcaccccgc ggtccatgga acaagaaaga gcgtcgcgca gccagcgcgg tgcgcagccg
     2701 agtgagctcg gcctccctcc cgaatatcgt tccattccgg tccaaggtct caggctgcat
     2761 cgctgctcgc aatctcttga atttcgacgc ccaaagcccg gagcctgtcc aacgcggcct
     2821 gcaccgcctc ttccccgtgc tgctggactt cgctggctcg attgaatgac cgttgcgctg
     2881 ccgccgcaag ttcgcgcagc aatccctgaa tttccaattc cagcttgatc cggctttgtg
     2941 ctgcgcgctc tgccacatcg ttctgcacgc gagatgcgtt gtgctccagc agtttgtcga
     3001 ggaacttatg tgcgtcccgc tcaatcggtc cgtaaaaatt caggaggccc agcaaaagat
     3061 cggtaatcat caggagggga gaagctggac gcgcaatgtg aatcatccga taaaactgaa
     3121 agcgggactt ctcgcgaaac cgttccggca tggagaacat gtcggagacg gaaccgacat
     3181 ccgtcactcc gctttctgcg gccctgtgaa tgaaatccag cgccatggct gcaaaccgcg
     3241 caacgctgcc ggagtattcc ttttccccga attcctgctc ctgtgtgagc cacggttcaa
     3301 tttgggaagc aacgatccgc tgcgcctcgg ccaacgcagc gcggcggaaa gtcacgccgg
     3361 aaccgcgcgc agtatgccga agaaaagatt ccaattcggc atgcacagcc ggagcaacgc
     3421 tttgaatgaa tcgagtgctg cgttccgcga agattctgga taagcgctgc tgttcggcgg
     3481 caaacagggg attgaggtct ttaagagagc gttcaacaga gactgccagt cgattgaggt
     3541 cgtcgatcct gcgttgtgat tcctcgcgtg gccggagcaa cgcccctcgc tcatcaacta
     3601 cgagttgccg tacctggccg gcaattcttg aggtgccgcg gtgcagagac tcttccacca
     3661 aggttcgccc agagttgcga ttgagtagct cgagagctgc cacgaagcca ttccaatcgc
     3721 ggccgcgctt accttcgagt tcttcgagag cgctcacttg ataaatgtgg ccgatcggtt
     3781 tgccgaggtc tcgttccagg atgcggcgcg cgaattctcc ggcggccgtc acttcgctgg
     3841 tgctgacgcg gtctgcctta ttgagcacga acagcatgtc ctttacgtgc tttgaagcat
     3901 gcttcaccag ctcgagttcg gtagcggaga gcggggggtc ggcgccgatc accacgatgg
     3961 cggcgtcgat atgcggaagg aaggttttgg tctcttcggt atttccggaa tagacggagc
     4021 ccaggcccgg tgtatccacc aggcacatgc ccgagcgcag acgcggattg cagacaaaca
     4081 cctcgacggc tgtgactcgc tttatgtttt ccgggttccg ttcctcggac acgtagtcca
     4141 cgatcgactc gggcgcgatc tcgtgccatt cgcgggagct attgcgaacg cgggcgcgga
     4201 gttcatttcc gtagcggatg atggtgggga ttgctgtaac tggtaggacg ccggcgggca
     4261 gcaaccgttg accgatcagg gcatcaagca gcgtcgattt gccgcgtttg aattggccga
     4321 cgcaggcgac gaagtagcgt ccctccgaca cccgctcggc caacgtgagc gcatcagacc
     4381 cgatccgatc cgctccaaac tcagcggcaa tttcggcgag ccggtggagc gacaggactc
     4441 gatctctctc agcgcggggc tcgctcctca tgcacatact tgatcacttc cacgtccgag
     4501 acgacgatca ggccttcctt cagcattggt tcaagcgccg gaatgagttt gtcaatcttc
     4561 tcttccgtat cgatgatgat cacggttatg ggagcgtcgg aggagaacga caaaagatgg
     4621 cgttcgcggc ggattcgggt gctggcgccg tatccaccga tcccgcgata aacagtggcg
     4681 ccggcgatgt cgagcttgcg acaggt
//
LOCUS       DATNDT010000143         3638 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1862435, whole genome shotgun sequence.
ACCESSION   DATNDT010000143 DATNDT010000000
VERSION     DATNDT010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1862435"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..869
                     /locus_tag="VN788_04690"
     CDS             <1..869
                     /locus_tag="VN788_04690"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020739514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HXU19861.1"
                     /translation="YRKTVSGFPRAKLCSMPFNGLRVIAFESRRSAEIAELIRRQQGE
                     PFTAPAIREEMLEDHQEAFDFAERLFRGEFDMVILMTGVGIRGLEKIVGSKYGEGRLP
                     QALRKITIVARGPKPAAVLREMQVPVTVQVPEPNTWRELLQVIEGRPERKIALQEYGR
                     SNDDLIAALRERGAQVTPMRIYLYQLPEDTAPLIEAARRIAQGEADILLFTTSVQAVH
                     LVDIAAEENIETRMRSKLREMVIASIGPTTSETLEEIGIAVDLEPSHPKMGFLVKETA
                     DRAAEILARKRV"
     gene            874..1077
                     /locus_tag="VN788_04695"
     CDS             874..1077
                     /locus_tag="VN788_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008870806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HXU19862.1"
                     /translation="MWKQFGKYYGLAILLPTCTLVGYIIGFLLDKGFGTRFFKLVFLL
                     LGIAAGFIELIRELNKDQDDGGS"
     gene            1061..1438
                     /locus_tag="VN788_04700"
     CDS             1061..1438
                     /locus_tag="VN788_04700"
                     /inference="COORDINATES: protein motif:HMM:NF015834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HXU19863.1"
                     /translation="MTAEADAGYERMLGRVAKILVILGVVGTAAFTVARGWRYGAGFL
                     LGAAISCGSLWRWQKVVNAIGVNPKRRSSWWLVLRLLVLGAAAYVIVRYLEVNVLAAL
                     VGLLASAAAVVIEILYELFTTRT"
     gene            1442..2179
                     /gene="atpB"
                     /locus_tag="VN788_04705"
     CDS             1442..2179
                     /gene="atpB"
                     /locus_tag="VN788_04705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HXU19864.1"
                     /translation="MPEHATWLTALFNQFLAAPADALLQAVHWRIVDPAHPWTNWMVM
                     ELLVVVITMITFAILRTRLSVDNPGKLQQTFELIHTFLVDQTAEADVPHGGKYLAFFG
                     TIFIFVLFMNLIGIIPTFEAPTMYPMVPAGLALAVFFYYNWMGMREHGLGKYLAHFAG
                     PMPLLAPLMIPIEIISHLARPLSLTIRLFANMFAGEKVTAAFLALTYFILPAAFMGLH
                     VFVSLLQAYIFALLAMIYVGGAVAHEH"
     gene            2285..2587
                     /locus_tag="VN788_04710"
     CDS             2285..2587
                     /locus_tag="VN788_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HXU19865.1"
                     /translation="MKWKLMMLMAVTAVLSSPLFAQGTQAATSPKDWKPVFAGIGMAI
                     ASGLCGLGQGKAVAGSAEAMARNPGATTAIRGALILGLVLIESLALYTLVIIFAIN"
     gene            2637..3521
                     /locus_tag="VN788_04715"
     CDS             2637..3521
                     /locus_tag="VN788_04715"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXU19866.1"
                     /translation="MKLHGIFPPLATPFDHHGDIWKVKVQHNVSKLNLTPVGGYVVGG
                     STGEVVMLTNDEMARAWEWVAEASAPEKILIAGTAAEGVRETLERTKRAAGIGYKVAL
                     VRTPAYYKSVVMKPETQMLFFRAVADQSPVPVLIYNFPQVTGIDLSVETVAALSEHPN
                     IAGIKESSGNQEKVQRLAMETKDGFNVLVGQAQTFASALNAGACGGILGFANAAPYAA
                     VSIWEAHRTREYAAALDWQKRISRPARLVTAKYSIPGLKHAMDLMGYYGGPPRLPLVP
                     IGPDAKQEIEEAFQDIRG"
BASE COUNT          747 a          969 c         1104 g          818 t
ORIGIN      
        1 gctaccgaaa gacggtgagt gggttcccgc gcgctaagct gtgttcaatg ccattcaatg
       61 gactgcgcgt aatcgcgttc gaaagccggc ggtccgccga gatcgcggag ttgatccggc
      121 ggcagcaggg tgagccattt accgcgccgg caatacgaga agagatgctc gaggatcatc
      181 aagaggcctt cgatttcgcg gagcgccttt ttcgtggcga attcgacatg gtgatcctga
      241 tgaccggcgt tggtattcgc gggctggaga agattgtcgg atcgaagtac ggagaaggcc
      301 gcttaccgca agcgttgcgg aagattacca tcgtcgcacg cgggccgaag ccggcggcgg
      361 ttctacgaga aatgcaggtg ccggtgaccg ttcaagttcc ggagccaaac acttggcgcg
      421 aactgctcca ggtaatcgaa ggccggccgg aacggaaaat agccttgcag gaatatgggc
      481 ggtctaacga cgatcttatc gcggcgttgc gcgagagagg cgcgcaggtg acgcccatgc
      541 gcatttatct gtaccagctt ccggaagaca cagcaccatt gatcgaggcc gcgcgccgga
      601 tcgcacaagg cgaagcggac atattattgt tcacaacttc ggtgcaggcc gtgcatctcg
      661 tcgatatagc ggcggaggaa aatatcgaaa ctcggatgcg gtcgaagctg cgcgaaatgg
      721 tgatcgcttc cattggacca acgaccagcg aaacgctgga agagatcggg atcgcggtgg
      781 atctagagcc gagccatccg aagatgggtt ttctggtgaa agagaccgcg gaccgcgcgg
      841 cggagatcct cgcccgcaag cgagtctgac ggcatgtgga aacagttcgg caaatactac
      901 ggcctcgcga ttctgctgcc cacctgcacg ctggtcgggt acatcatcgg gtttctgctt
      961 gacaagggat tcggcacgcg atttttcaag ttagtgtttc tgctgcttgg aatcgcggcc
     1021 ggatttatcg agctgatccg cgaactaaac aaggaccagg atgacggcgg aagctgacgc
     1081 cggttacgag cggatgctcg gacgcgtcgc gaaaattctg gtcatcctgg gcgttgtggg
     1141 aacggctgca ttcaccgttg cgcgcggctg gcgatatggc gccggatttc tgctgggcgc
     1201 cgcgatttcg tgcggtagcc tgtggcgttg gcagaaggtt gtgaacgcca tcggcgtcaa
     1261 tccgaagcgc cgttcctcgt ggtggctggt gctgcgtctg ctggtgttgg gcgctgccgc
     1321 ctatgttata gtcagatatt tagaagtcaa cgttctggcg gctctcgttg gccttttggc
     1381 gagtgcggcg gctgttgtaa tcgaaatcct ttacgagctt ttcacgaccc gaacctgaac
     1441 tatgcctgaa cacgcaacct ggctgactgc tctgtttaac cagttcctcg cggccccagc
     1501 ggacgctctg ctccaggccg tacattggcg catcgtcgat ccggcccatc cgtggacaaa
     1561 ctggatggtg atggagctgc ttgtggtggt catcacgatg atcacttttg cgattctgcg
     1621 cacgcgcctt tccgtcgata atccaggaaa attacagcag acctttgagc tgatccacac
     1681 attcctggtc gatcagaccg ccgaagcgga tgtcccccat ggtggaaagt atcttgcctt
     1741 tttcgggact atttttattt ttgttctttt catgaacctc atcgggatca tcccgacatt
     1801 cgaggctccc accatgtacc cgatggtccc ggccggcctc gcattggctg ttttctttta
     1861 ttacaactgg atgggcatgc gcgagcatgg cctgggcaag tatctggccc acttcgccgg
     1921 tccaatgccg cttctcgcgc cgttgatgat tccaattgaa atcatcagtc acctggcgcg
     1981 cccgctctcg ctgacaattc gtctttttgc gaacatgttc gcgggcgaga aagtgactgc
     2041 cgcgtttctt gcattgactt actttattct tcccgcggcg ttcatgggcc tgcatgtgtt
     2101 tgtaagtttg ctgcaggcgt atattttcgc gctgctggcc atgatctacg tgggcggcgc
     2161 ggtggcacac gaacattgat actcatagcg gctttatgca gacctcaggc gtgagcttcc
     2221 gtttccccag aagcggtaag gaccacctcc ctggaggaat ttattcaaga aggagaaccc
     2281 acttatgaag tggaaattga tgatgctgat ggcggtgaca gctgtgctgt cgagcccgct
     2341 gttcgcgcaa ggcacacaag ctgcaacaag cccgaaagac tggaagccgg tgttcgctgg
     2401 aatcgggatg gccatcgcat ccggtctttg tggcctcggc caaggcaaag cggttgccgg
     2461 atcggctgag gcgatggcgc ggaatccggg agctacgact gcaatccgcg gcgcgctgat
     2521 tctcggcctg gtgctgatcg agtctctcgc gctctacacg ttggttatta ttttcgccat
     2581 caactaataa gagatggcgt ctgaaagagg ggcgggtcac taacccgccc tcacttatga
     2641 aactgcacgg cattttccct ccgctcgcga ctccgttcga tcaccatggc gatatctgga
     2701 aagtcaaagt tcaacacaac gtttccaagc tcaacctgac gccggtcggc ggatatgtcg
     2761 ttggcggctc tacgggtgaa gtggtcatgc tcacgaatga tgagatggcg cgggcctggg
     2821 agtgggtcgc ggaagcctcg gctccagaga aaatcctgat cgcgggtacg gcggccgagg
     2881 gcgtccggga aactctggag cgaaccaagc gcgccgctgg aatcggttat aaagtcgcgc
     2941 tggtccgcac gccggcttat tacaaaagcg tcgtgatgaa gcccgagacg cagatgctct
     3001 tttttcgggc ggtggcggat caatcgccgg tgccggtgtt gatctacaat ttcccgcagg
     3061 ttactggcat cgatctttcc gttgaaactg tagccgcatt atcggagcat ccgaacatcg
     3121 ccggcatcaa ggaaagctcg gggaatcagg aaaaagtgca aaggcttgca atggaaacaa
     3181 aggacggttt taatgtgctt gtagggcagg cgcaaacgtt tgcgtcagct ttaaatgcag
     3241 gtgcatgcgg gggtattctc ggttttgcaa acgctgcccc atatgccgct gtgtcgatct
     3301 gggaggcgca tcggacgcgc gagtacgcgg cagcgctcga ttggcaaaag cgaatcagcc
     3361 gccctgcgcg cctggtgacc gcgaaataca gcattcctgg tctcaagcac gcgatggacc
     3421 tgatgggata ttacggcggt ccgccgaggc tgccgcttgt gccgattggt ccggacgcga
     3481 agcaggaaat cgaagaggca ttccaggata tccgcggcta ggccggccgc atctctaagg
     3541 aacgcgtcta catcaaagat ggcggcgcgt acagacgcga tctcgttatt tttccacccg
     3601 taaacctggt ctcggactcg atttctcgct tgagggaa
//
LOCUS       DATNDT010000144         4744 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4308998, whole genome shotgun sequence.
ACCESSION   DATNDT010000144 DATNDT010000000
VERSION     DATNDT010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4308998"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            306..1559
                     /locus_tag="VN788_04720"
     CDS             306..1559
                     /locus_tag="VN788_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HXU19867.1"
                     /translation="MNAEIIAIGSELLTPHRLDTNSLFITNELNQVGVRVVHKAVVGD
                     TPDEMMSSFRHALDRADVVVSCGGLGPTDDDRTRQTVSELLGRKLLRNEEVLQKLQAR
                     FRRFGRTMPEINTRQALVIEGATVMPNPRGTAPGQWIEAKGHILILLPGVPSEMRALM
                     ETEVKPRLAKLGIRERRYARDLRIVGLFESEVEQRVSPLYAQYPDTDTTILATAGGIQ
                     LHPSIWSDDPAVAQARLDELVGRMALVLGDNLISTCGEVIEDIVGRLLMENRATIAVA
                     ESCTGGLLAERLTNLPGSSAYFLGGIVCYSNDLKTRLVGVPAEMIEAKGAVSSEVALA
                     LAAGIRKLTGATLGIGITGVAGPGGGTPEKPVGLVHIGIADEQGPREKSFQFPGDRER
                     IRFFATQNALDAVRRHFLFPSHGYA"
     gene            1761..2366
                     /gene="thpR"
                     /locus_tag="VN788_04725"
     CDS             1761..2366
                     /gene="thpR"
                     /locus_tag="VN788_04725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HXU19868.1"
                     /translation="MRLFVALDLSDEVRRALGDLIAGLKPLCKDARWVRPEAMHVTLK
                     FIGHAVPTGDTGKLDAVRASLATVHSGRPVEMHFRGVGFFPNASRPRVVWCGVQASEN
                     LAPLAADIDKVLEPLGIPGEDRAFLPHLTLARMKAARSGGRSRSNRGNSEGLRSAATE
                     MSEKNFGAAREAEFHLFESITKPGGSEYRKLESYSFVKARE"
     gene            2363..2974
                     /gene="plsY"
                     /locus_tag="VN788_04730"
     CDS             2363..2974
                     /gene="plsY"
                     /locus_tag="VN788_04730"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HXU19869.1"
                     /translation="MSAHWLLIVAAYLLGSIPFAFLIVKALGGDDIRSTGSGNIGAAN
                     VVRNAGWLAGLLTLFFDAGKGYLAVWIAGHYTNGSGRWMIAAAVAAVAGHIFPVWLRF
                     KGGKGVATSLGAFLPITAGAVAAAGALWLLVVLFWRYSSLGSIVAAASLPALVYLFYA
                     PRHAPPTYMLAGTVVISLLVLAKHQANIRRLIAGTESRLGSRR"
     gene            3066..>4744
                     /locus_tag="VN788_04735"
     CDS             3066..>4744
                     /locus_tag="VN788_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HXU19870.1"
                     /translation="MSIKSLTHLSTRRLRILWIVLGALLLVSITPLWLYHRQALKLSE
                     DKLQDTERVQQSEITRSLATETLQFEQGLRGQLLSERQLLALTGWIENVDDPSHSPQL
                     SRLLQDFAENNPNILYVTAVGKQAKGQSAGSFNADQDPFVQAALKRAFTSSIQDFDSI
                     SEPFALGQDNRPALVVSIPLLSAGQFAGMVAAVVSLEQLQARLRDASVRDRTVYIVDL
                     DGRIVAYPNTRTLVPGSDVKSTSPLAKQVSELPQDLRATQTTNFQMRSKDGRHSIEMI
                     GTYSTIPELRWAVIAQRSLDDARVDAGVTELTSEALQFVVGVTFAALILGYLFAVGIT
                     RPIRGLVQSTRAISRAEFHERVEVRGAAEISELAETFNHMAGDIEEHVEKLQQAAEEN
                     RELFLGSIKMLAAAIDEKDPYTRGHSGRVAKYSSIIGEGLGMNAEDLDRLRISALLHD
                     VGKIGVDDRVLKKPGQLTEEEFELMKQHPSKGANIMRPVAQLKEMLPGIELHHERMDG
                     GGYPYGLQGDQIPMMARIIAVADTLDAITTNRPYQSAMDLEYALGRIRSLAV"
BASE COUNT          958 a         1269 c         1556 g          961 t
ORIGIN      
        1 gacgccgcaa gcggaagctg aagtggtgct cggcggaagc aatatcgtgg gcgtcgcgct
       61 tcttcccacg catcgtgcgg tggtggcaac taatagtgca ctgtttaccc tcgactggga
      121 cgtcgaggga ctcccattga acggatagag gtcgcggcgc gtcctctggc tcgacctttg
      181 attggtgtga cctgcctctt caaatgtcaa atagctgtgc atgccgagaa atacccacga
      241 tgagagagcg ttcgtcgtgg gtacccttgc acttgcttgc gatcatcgag taaaactctt
      301 agacaatgaa tgcggagatt atcgcgatcg gttcggaact gctgacgccc caccgtttgg
      361 ataccaattc actttttatt acgaatgaat tgaatcaggt gggcgtgcgc gtggtgcaca
      421 aggcggttgt gggagacacg ccggacgaaa tgatgtcgtc gttccgacac gcgctcgatc
      481 gagccgatgt ggtggtgtcg tgcggcggcc tgggaccgac cgacgacgat cgcacgcggc
      541 agactgtgtc ggaattgttg gggcgcaagc tgctgcgaaa cgaagaggtg ttgcagaagc
      601 tgcaggcgcg gtttcgtaga ttcggacgca cgatgcccga aatcaacacc aggcaggctc
      661 tggtgatcga aggggcgacg gtgatgccta atccgcgcgg caccgcgcca ggacaatgga
      721 ttgaagcgaa gggacacatt ttaattttgc ttcccggcgt gccgagtgaa atgcgcgcgc
      781 tgatggaaac cgaggtcaag ccgcggctgg cgaaactggg gatcagggag cggcgttatg
      841 cgcgcgacct tcgcatcgtc ggtttgtttg aatcggaggt ggagcagcgc gtctcaccgt
      901 tgtacgcgca gtatccagat acggacacca ccattctggc gacagctggc ggcatccagc
      961 ttcatccgag catctggtcg gacgaccctg cagtcgccca ggcacggctc gacgagctgg
     1021 tgggacgcat ggcactggtg ctcggcgata acttaatttc cacatgcggt gaggtgattg
     1081 aagacattgt ggggcgcttg ctgatggaga atcgcgcaac gattgccgtc gcggaaagct
     1141 gcaccggtgg attattggcc gagcggctga ccaatcttcc gggtagctcg gcatattttt
     1201 tgggcgggat cgtttgctac agcaatgatt tgaagaccag gttggtgggc gtgcccgcgg
     1261 agatgatcga agcgaaggga gctgtgagct cggaagtggc gctggcgctc gcggcgggca
     1321 ttcgcaaact tacgggcgca acgctgggca tcggaatcac gggcgtcgcg ggtccgggcg
     1381 gcggcactcc ggagaaaccg gtcggcctgg tgcatatcgg aatcgcggat gagcaggggc
     1441 cgcgcgaaaa aagttttcaa tttcccggcg accgggagcg catccgattt ttcgcaacgc
     1501 agaacgcgct ggacgcggtg cggcgacatt tcctgtttcc gtcgcatggc tacgcgtaag
     1561 ttacatcgaa gagagattct tcgactgctt ccacgccgcg cgcaaaccgc gcggcgagta
     1621 aaagcatcgg actccgctcc cttcctgcgt caggggacat tcgctgcgct cactgcaagc
     1681 aggtaggatg acgaaaatga ggcggcgcta cggtgcgttt cacgtagtct ccacggcctc
     1741 tgaaaaacgt gtgagaaaac atgaggctgt ttgtggcgct cgatttatct gatgaggtgc
     1801 ggcgcgcgct cggggatttg atcgcgggac tgaaaccgtt gtgtaaggac gcccggtggg
     1861 tgcggccgga agcgatgcac gttacgctga aattcatcgg acacgcggtg ccaaccggag
     1921 acacgggaaa acttgacgcg gtgcgcgcgt cgctggccac cgttcactcg ggccgacctg
     1981 tagaaatgca tttccgcggc gtgggattct ttccgaatgc gtcgcgtccg cgagtggtgt
     2041 ggtgcggcgt gcaagcgtcg gaaaatctgg cgccactggc tgctgacatc gataaagtgc
     2101 tcgagccgct ggggattcct ggcgaagacc gagccttctt gccgcacttg acgctagctc
     2161 gcatgaaggc ggcgcgctcg ggcggacgta gccgttcgaa ccgggggaac agcgaagggc
     2221 tgcggtccgc cgccacggaa atgtcggaaa agaatttcgg tgcggctcgc gaggcggaat
     2281 ttcatctttt tgaaagcatt acgaaacccg gaggatcgga gtatcggaaa ttggagagct
     2341 attcgttcgt gaaggcacgc gagtgagcgc ccattggctc ctgatcgtcg ccgcatatct
     2401 gctggggtcg atcccctttg cgtttttaat cgtcaaagcg ctgggcggcg atgacatacg
     2461 ctcgacaggc agcgggaata tcggcgctgc caacgtggta agaaatgcgg gctggttggc
     2521 gggactgctt acgctatttt tcgatgcggg caagggctac ctggcggtgt ggatcgcggg
     2581 ccactacacg aatggcagcg gacgatggat gatcgctgcg gcggtggcgg ccgttgccgg
     2641 tcatattttt ccggtctggc tgagattcaa aggcggcaag ggagtggcga ccagtctggg
     2701 agcctttctg cccatcaccg cgggtgcggt ggcggctgcg ggcgctctct ggttgctggt
     2761 ggtcttgttt tggcgttact cgtcactggg ttccattgtg gcggcggcgt cgctgcccgc
     2821 gctcgtttat ttgttttacg cgccgcggca tgcgcccccg acctacatgc tggcgggcac
     2881 ggtggtgatc tcgctgctgg tcctcgccaa gcatcaagcc aatatacgac gcctgatcgc
     2941 cggcaccgag agccggctgg gttcgcggcg ctagctaaaa ggcgtggctt atacggcacg
     3001 cgcggtacca tcgtttgatt gtcgcttcca tccggctccg atagcatcat tgggcaggta
     3061 gtttcatgtc cattaagtcc ttaacgcatc tttcgacgcg gcgattacga atcctgtgga
     3121 tcgtgctagg cgcacttttg ctcgtcagca tcacaccgct gtggctctac catcggcaag
     3181 cgctgaaact tagcgaggat aagctacagg atacggagcg cgtgcaacaa agcgaaatca
     3241 cgcgttcgct ggctactgaa actttgcaat tcgaacaggg gctgcggggg caactgctga
     3301 gcgagcggca gcttcttgcg ctaacagggt ggatcgagaa cgtggacgat ccgagccatt
     3361 cgccgcagct ttcgcggctg ctgcaagatt tcgcggaaaa caatccgaat attctgtacg
     3421 taacggcggt agggaagcag gctaaaggac aatcggccgg cagcttcaat gcggaccagg
     3481 atccattcgt tcaggcggca ctgaagcgcg catttacttc gagcattcag gatttcgatt
     3541 cgatcagcga gccctttgcg ctggggcagg acaaccgtcc cgcgctggtg gtttcgatac
     3601 cgttgctctc ggccgggcag ttcgcgggaa tggtggccgc ggtggtgtca ctcgagcagt
     3661 tgcaggcccg gctgcgggat gccagcgtgc gcgacaggac ggtatacatt gtggatttgg
     3721 atgggcggat cgttgcctac ccgaatacgc ggacattggt gccggggagc gatgtgaagt
     3781 caacttcgcc gctggccaag caagtgagtg aattgccgca agacctccga gcaactcaaa
     3841 cgactaactt ccagatgcgt tcgaaggacg ggcgccattc catcgaaatg attggaacct
     3901 atagcacgat cccggagttg cgatgggcgg tgatcgcgca gcgcagcctg gacgacgcgc
     3961 gcgtggacgc cggcgtaacc gagctgacct cggaggcgct gcaattcgtg gtcggcgtca
     4021 catttgccgc gctgatcctg ggatatctct tcgccgtggg gatcacgcga ccgattcgcg
     4081 gcttggtgca atcgacacgg gccatcagcc gcgcggagtt tcatgaacgc gtggaggttc
     4141 gcggcgccgc cgagatcagc gaactcgccg aaacgttcaa ccacatggcg ggcgacatcg
     4201 aagagcacgt tgagaaactg caacaggccg cagaggaaaa tcgcgaactg tttttgggct
     4261 cgatcaaaat gctggccgcg gccatcgacg aaaaggatcc ttatacgcgc ggccactccg
     4321 ggcgcgtggc taaatattcg agcatcatcg gtgagggcct gggaatgaac gcggaagacc
     4381 tggaccgatt gcgcatctcc gcgctcttgc acgacgtggg caaaatcggc gtggacgacc
     4441 gcgtgctgaa aaagccgggc cagttgacgg aggaagaatt tgaactgatg aagcagcatc
     4501 cctcaaaagg cgcgaacatc atgcggcctg tggcgcaatt gaaggaaatg cttccgggca
     4561 tcgagctgca tcacgagcgc atggacggcg gcgggtatcc atatggcctg caaggcgacc
     4621 agattccgat gatggcacgc atcatcgccg tagccgacac gctcgacgcg attaccacca
     4681 accggccata tcagtccgca atggacctgg aatatgcgct gggccgcatc cgatcgctgg
     4741 ctgt
//
LOCUS       DATNDT010000145         3341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1329908, whole genome shotgun sequence.
ACCESSION   DATNDT010000145 DATNDT010000000
VERSION     DATNDT010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1329908"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..382
                     /gene="ssrA"
                     /locus_tag="VN788_04740"
     tmRNA           29..382
                     /gene="ssrA"
                     /locus_tag="VN788_04740"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(391..2676)
                     /locus_tag="VN788_04745"
     CDS             complement(391..2676)
                     /locus_tag="VN788_04745"
                     /inference="COORDINATES: protein motif:HMM:NF023418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HXU19871.1"
                     /translation="MVSAPSPTMLHGSTPVGTLPAVERYFQVSLYLLVATGVLAIVST
                     GKLDLFSTMAPVGALLYKGIRIRRGLGPELSSRAATWCVLGYFLFFPLDLWVLSRGLA
                     VGAPNPPLYAALLSAIHLVIFATLIRLYSARTNRDFAFLAVLAVASMLVSAILTVETG
                     FLIALAAFLVLSVSTFVALEIRRSAAGAVSPALELDSTAAHKLNRALGITSALVAGSV
                     LIFGALIFFLIPRFTTGYLSALNLQPSLMTGFSDDVALGQIGELKKNTAVVLRIRVDG
                     DPARASDIHWRGIVLTNFDGSRWFTPTQDQTVVMPNPDGDYRFAFPRSDRENFYSLHY
                     TVLMEPVATDAIFVAPQVATLHGQFGNSEASRQDTLPHRGLGLLFIDRTGSIFNSARN
                     SAKIRYEGLSYLPTIPPSDLRKAPSTFPPAIEATYLQLPVLDPRIPKLAADATASLKN
                     EYDKASAIRGYLTSHYSYTLDLSGSHGTDPLANFLFVRRAGHCEYFASAMTVMLRSIG
                     IPARYVTGFLPGEYNDLGGDYIIRQSDAHSWVEVYFPTYGWVTFDPTPSVSGNHTGVL
                     SRISLYWDWLQLTWGEWIINYDFSHQLALGQNIHKYSRGWSDRAREIYRQKEDAMMRG
                     LLAADRRAEASPYLLPGVLIFLIAILFAMRGRAVIRYAVARWALRTKRGGNLTASLAT
                     LEYAEMLRLLEARGWKKSLSQTPLEFATAVPADLSAQILQFTEIYQAARFGSQPAPIE
                     RMSSLLRLIKSALRSGAASHQ"
     gene            complement(2787..>3341)
                     /locus_tag="VN788_04750"
     CDS             complement(2787..>3341)
                     /locus_tag="VN788_04750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19872.1"
                     /translation="LLAAHAPGDWNQALMELGETICTPQIPRCGLCPVSRWCLAHKRG
                     LAGKIPAGRRKRASVKVRIAAAVLRDPRGRTILVRNPGAHHDALFSRMWQFPAVEITH
                     DPATEVKAHLGEMLRIDTASLELTPLQAVRHGVTFRNITFMPFLVRVSRLPKLPRTRI
                     APLANLGRIPVSSATRKIAATALP"
BASE COUNT          788 a          892 c         1042 g          619 t
ORIGIN      
        1 aggagagtgg tattctggcg gttccaacgg gggcgacacg gtttcgacgg aagctgtcgc
       61 ggcccgaagg catgccgggc gccacctgcc cgtaaccggg tggaacaaca caactggcaa
      121 ctcacagttg gctctcgctg cctaaccaaa agtaggtagc gcgtctcctc aggcgtcgcc
      181 tgcgcgcttg agatgaggcg tcacacagta ggctggccgt cacatttcgg tccgtaatgt
      241 ggcggcgaga ccatcggact agccaggggc gcgtcttgcc cgctctgagt tcctctggcg
      301 aaaccttcgg aacgggacaa gcatgtagtc ttcgggtggc aaggcttctc ggacgggggt
      361 tcaactcccc ccgcctccat cgttttccac ttattgatgc gaagcggcgc ctgatcgcag
      421 cgcgcttttg attagccgca agagcgacga cattcgctca attggggccg gctggctgcc
      481 gaagcgagca gcctggtaga tttccgtgaa ctgcaagatc tgcgccgaaa gatcagcagg
      541 aactgcggtg gcgaattcga gcggagtctg cgaaagagat ttcttccagc cacgtgcttc
      601 caacaggcga agcatttctg cgtattcaag agtcgcgaga gacgccgtga ggtttcctcc
      661 tcgttttgtg cggagggccc agcgtgcaac cgcgtagcga ataacggcgc gaccgcgcat
      721 ggcaaacaaa atagctatga gaaaaattaa aacgcccggc agaagataag gggaagcctc
      781 tgcccggcgg tccgcggcaa gaaggccccg catcattgca tcttcctttt gacggtagat
      841 ctccctggcg cgatcgctcc agccccgtga gtacttgtgg atgttttgtc ctagcgccag
      901 ttgatgcgaa aaatcatagt tgatgatcca ttcgccccag gtgagctgaa gccagtccca
      961 ataaaggctt atgcgagaga gaacgccggt gtggtttcca ctaacagaag gcgtgggatc
     1021 gaacgttacc cagccgtaag taggaaagta aacttccacc cagctgtgcg catcgctttg
     1081 ccggatgatg taatcgccgc cgaggtcgtt gtactcgccg ggcaaaaagc cggtgacgta
     1141 gcgcgcgggg attccgatgg aacggagcat cactgtcatc gccgaagcga agtattcgca
     1201 gtggccggcg cggcgaacaa acagaaaatt ggcgagaggg tcggttccat gagagccaga
     1261 aagatccagg gtgtaggagt agtgcgaggt gagatagcct cggatcgcgg aggctttgtc
     1321 gtattcattt ttcagagagg cagtcgcgtc cgccgcaagc tttggaatgc gtgggtcgag
     1381 gaccggcaat tgcagataag tggcctcaat tgccggcgga aaagtggacg gagccttgcg
     1441 cagatcggaa ggcggtattg tcggaagata ggacagcccc tcataacgaa ttttggcgct
     1501 gttacgggct gaattaaaaa tcgagccagt tcggtcgatg aacaataacc ctaaccctct
     1561 gtgcggcaat gtgtcctggc ggctcgcttc gctattgcca aattggccgt ggagggtcgc
     1621 cacctggggc gcgacaaaaa tcgcatcggt agcgactggc tccatgagaa ccgtatagtg
     1681 aagcgaatag aaattctcgc gatcggagcg gggaaacgca aagcggtagt ccccgtccgg
     1741 attgggcatg acgaccgttt gatcctgcgt cggcgtaaac caccgactgc cgtcgaagtt
     1801 ggtaagcaca atgccgcgcc agtggatatc ggatgctcgt gcgggatcgc catccacgcg
     1861 gatgcgcaat acaaccgcgg tgttcttctt cagttcgcct atttggccga gagcgacatc
     1921 atcgctaaag cccgtcatca agctgggttg caggttgagg gcactgaggt atccggttgt
     1981 aaaacgagga atcagaaaaa agatcagggc gccgaaaatc agcacgctgc cggccacgag
     2041 ggccgacgtg atgcctagtg ctcggtttag tttatgagcg gccgtcgaat cgagttcgag
     2101 agccggcgac acagcacctg ccgcgctacg ccggatttcg agcgcgacaa acgtcgaaac
     2161 ggacaagacc aggaatgctg caagcgctat caggaaccca gtttcgactg tgaggatggc
     2221 ggaaacgagc atggatgcaa cagccagcac ggcgaggaaa gcgaagtcac ggttcgttcg
     2281 cgccgaatag aggcgaatca gcgtcgcgaa aatgactaaa tgaattgcag agagcagtgc
     2341 cgcgtagagt ggaggattcg gagcgccgac ggccaaaccg cgcgaaagca cccaaagatc
     2401 caatggaaaa aaaagaaagt acccgaggac acaccacgtg gccgcacgcg acgaaagttc
     2461 ggggccgagg cctcggcgga tgcggatgcc cttatagagc agcgcgccca ctggggccat
     2521 agtcgaaaaa aggtcgagct tgccggtcga aacgattgcc agcacgccgg tagctaccag
     2581 aaggtagagc gatacttgaa aatatcgttc cacagcggga agcgtgccga cgggcgtact
     2641 accgtgcagc attgttggtg aaggcgcgga aaccatctcg tccttaagat gctatcactc
     2701 cgcgctctac cctggattga aggcacgaca aacgatgcca atggggcaac aagggcgctt
     2761 gatggaatac ggacggcgcg atgctatcag ggcaaagccg tagccgcgat tttgcgcgtg
     2821 gcgctggaaa cgggtatgcg cccaagattg gcaagcggag cgattcgcgt acggggaagc
     2881 tttggaagcc gcgacactcg cactagaaag ggcataaatg tgatgttgcg aaatgtgact
     2941 ccgtggcgca ctgcttgtag cggcgtaagc tccaaagagg ccgtatcgat tcgcagcatt
     3001 tcaccgaggt gagccttaac ctctgtcgca gggtcatgcg taatttcgac ggcgggaaac
     3061 tgccacatac gggaaaaaag tgcatcgtga tgcgcgccgg ggttgcgaac taagatcgtc
     3121 cgcccacgag ggtcgcgcag gaccgcagcc gcaatgcgca ccttcacgct ggcgcgcttc
     3181 cgacgcccgg ccgggatctt acctgcgagg ccgcgcttat gcgcgagaca ccatcgtgat
     3241 accgggcata aaccacaacg cggaatttgc ggcgtgcaga ttgtctcccc gagttccatc
     3301 aacgcctggt tccaatcccc cggcgcgtgg gcggcaagga g
//
LOCUS       DATNDT010000146         2566 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1757056, whole genome shotgun sequence.
ACCESSION   DATNDT010000146 DATNDT010000000
VERSION     DATNDT010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1757056"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VN788_04755"
     CDS             <1..799
                     /locus_tag="VN788_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXU19873.1"
                     /translation="IQGDAPAWIHNAPAPHQATDAKTAAALRGMMEDVMIEGTGKHSQ
                     LDGYTSGGKSGTAQQIDPATGRYSATRYNASFVGFAPVNNPAVTILAVLDSPVGPHHG
                     GQVGGPIFKRVAEQVLAYLGVPHDVPSPSDVETAKNEARRRGSDVTSQDAKTDDSTRE
                     SFAASIGMTRKMEAAPIAAFAEQDFIAVPSLAGESVRGVTEECSRLGLAPTLIGNGIA
                     IEQFPEAGTQVQRGSRVTVRFGRSAELEPVAARGSGNRGSVRTQRRR"
     gene            838..2328
                     /locus_tag="VN788_04760"
     CDS             838..2328
                     /locus_tag="VN788_04760"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXU19874.1"
                     /translation="MKIQRLLDGVEVLKIVGTADAEILSLAYDSRRVGPGAIFFALRG
                     EKTNGAKFITDALQGGAIALASEGPRPDPLPSHITWVELAPGSGRRGLARAASNFYRH
                     PADGLQLISMTGTNGKTTTAFLVDSILREAGFRPGLIGTIEHRTPKRSRAAVNTTPES
                     ADLQQMFAEIRDSGGTHVVLEASSHALAMERLWGCHFAVAIFTNLTRDHMDYHQTFEE
                     YFAAKKTLFEGTGAGAPGIGVINADDPYAARLTGLAQRTLTYGLKGAPEVTARKFVLS
                     FDGLEFVANTPIGNFEVRSPLVGRFNVYNILGAIGAGIGLDIPKGKIEDGIARLQSVP
                     GRFQRIDEGQPFRVIVDYAHTDDALRNLITTARELGPSARIITVFGAGGERDRSKRPL
                     MGEVAGSLSDLVVLTSDNPRGEDPLRIINDVVVGLQKSSAKYRIEPDREQAIVGAVEE
                     ARPGDTVLLAGKGHESYQVLRDRTVEFDDREKARAVLHSKGYSKTH"
     gene            2375..>2566
                     /locus_tag="VN788_04765"
     CDS             2375..>2566
                     /locus_tag="VN788_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HXU19875.1"
                     /translation="MRWTAVQVSEALGVPLAQRIEPSAIIGGVSIDSRTVGAGELFVA
                     IRGPRHDGHAFVASALERGA"
BASE COUNT          565 a          675 c          839 g          487 t
ORIGIN      
        1 gattcagggc gacgcgcctg catggattca caacgcgccg gctcctcatc aggctaccga
       61 cgcgaagacg gctgccgctc tgcgcggaat gatggaagat gtgatgattg aggggaccgg
      121 caagcactct cagctcgatg gatacacgtc cggagggaag tcgggcacgg cgcagcagat
      181 cgatcctgca acgggccgct attcggcaac ccgttacaat gcgtctttcg tgggttttgc
      241 gccggtgaac aacccggcgg tcacgatttt ggccgtgttg gattcgccag tgggtccgca
      301 tcatggaggg caggtcggag gcccgatttt caagcgcgtg gccgaacaag tattagcgta
      361 tctgggcgtg ccacacgatg tgccgtcgcc gtccgatgtg gaaacagcaa agaacgaggc
      421 gcggaggcgc gggtctgacg tcacgtcaca agacgcgaag accgacgatt cgacacgaga
      481 gagttttgct gcatctatag gaatgactcg aaagatggaa gccgcaccca tcgcggcgtt
      541 tgctgaacag gatttcattg ccgttcccag ccttgccggg gagagtgtgc gaggtgtcac
      601 cgaggaatgc tcgagattgg gcctagctcc gacgctaata ggcaacggca tagctatcga
      661 gcagtttccc gaagccggga cccaggtgca gcgcggcagt cgagtgacgg ttcggttcgg
      721 acgttccgcc gaactcgagc cggtggcggc tcgggggagt gggaatcgag gttcggtgcg
      781 aacccaaagg cgcagatgag aaaaaacggg cgaaccgaca cactagaagc acgcggcatg
      841 aaaattcaaa gattgctgga cggcgtcgag gtgctcaaaa tcgtcggcac ggccgatgct
      901 gagatcttat cgctggctta cgacagccgc cgcgtcggcc caggagccat tttcttcgca
      961 cttcgcggcg aaaagacgaa cggagcaaag ttcatcaccg acgctttgca gggcggcgcg
     1021 attgctttgg cgagcgaagg ccctcggcca gatccactgc cctcgcacat tacctgggtc
     1081 gagttggctc caggctcagg gcgacgtggc ttggcacgag cggcctcgaa tttctaccgg
     1141 catccagccg atggtctgca gttaatcagc atgacgggaa cgaacgggaa gaccacgacc
     1201 gcatttcttg tggactcgat cctaagagaa gcgggcttta ggccggggct gatcgggaca
     1261 atagagcatc ggacgccgaa acgaagccgc gccgccgtaa acactacgcc cgaatcagcc
     1321 gatctgcagc agatgttcgc tgaaatccgc gactcgggcg gcacccatgt ggtactggaa
     1381 gcgagttcgc acgcgcttgc aatggaacgg ttgtggggct gccatttcgc tgtcgcgatt
     1441 tttacgaatt tgacgcgcga tcatatggat tatcaccaaa cgttcgagga gtacttcgcc
     1501 gcgaagaaga ccttattcga aggcacgggc gcaggggcgc cagggattgg ggtgatcaat
     1561 gcggacgatc cctatgcagc gcggcttacc ggattggctc agcgcacgct gacgtacggg
     1621 ttgaagggcg cccctgaggt gactgcacgc aaatttgtgc tctcgttcga cggacttgaa
     1681 ttcgtggcga acacgcccat tggaaatttt gaggtgcgct ccccgctcgt tgggcgcttc
     1741 aacgtttaca acatactggg tgcgatcgga gctgggattg gattggacat tccgaagggc
     1801 aaaatcgagg atgggattgc gaggctccaa tcggttccgg gacggtttca acgcatcgac
     1861 gagggacagc catttcgtgt aattgttgat tacgcgcaca cggacgacgc gctgcggaac
     1921 ctgataacca cggcccggga gttaggacct tcggcacgga tcatcacggt atttggcgcg
     1981 ggcggggagc gagatcgtag caaacggccg ctgatgggtg aagtggcagg ttccttgagc
     2041 gatctggttg tgctgactag cgacaatccg cgaggcgagg atccgcttcg gatcataaat
     2101 gatgtagtag taggacttca aaaatcgagc gcgaaatatc gaatcgagcc agaccgcgaa
     2161 caggcgattg tcggcgcagt ggaagaagcg cgcccaggcg acaccgtact gctcgcgggc
     2221 aaaggccacg aaagctatca ggtgctgcgc gatcggaccg ttgaattcga cgaccgcgaa
     2281 aaagcgcggg ctgtcctgca tagcaaaggc tattcgaaaa cgcactgaat gcagattggc
     2341 gcgacgaagc ggtggaaagt caattggaga tacgatgcgg tggacagcag ttcaagtttc
     2401 tgaagccctg ggcgttccac tggcgcagcg gattgagccg tcggccataa tcggtggcgt
     2461 atccatcgat tctcggaccg tgggtgctgg ggaactgttt gtggctatac gcggaccgcg
     2521 acacgatggg cacgctttcg tggcctcggc attggagcgc ggcgcc
//
LOCUS       DATNDT010000147         4692 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2614954, whole genome shotgun sequence.
ACCESSION   DATNDT010000147 DATNDT010000000
VERSION     DATNDT010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2614954"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1600)
                     /gene="aroE"
                     /locus_tag="VN788_04770"
     CDS             complement(62..1600)
                     /gene="aroE"
                     /locus_tag="VN788_04770"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HXU19876.1"
                     /translation="MKLLGLNKICAVIAAPDAEAMGKQLTKALRLTRMAELRLDWLSD
                     DREISIFLRRLATSRPRAILIATCRRREAGGRYRGTISNQLAHLAQAIRAGCVWYDLE
                     IETVRKCSQALLSGLLGQGRQLTSAHFFYEPPKDLTAVAAEMARNQSDAIKIAMKCNS
                     LAESRRLLGFAEKRRNTIAVPMGDVAQPARLLALRGTNGFAYAPVENSTAPGQISLDE
                     MKMIYRADQVTARTRVYGVVGDPITHSLSPAMQNAGFSARRMNAVYLPFLTHDLRDFV
                     DSIEPLGIKGFSVTLPLKERILRFLHRCDPLAEKIGAVNTVAVRNGGKLYGYNTDYVG
                     ILRTLERRTKLHGSRVLIVGAGGAARSVAFALAQAGAAVFVSARRYKRAKHLARAAGG
                     EAIARTALRHTAFDVIVNTTPVGMYPDVSTSPLEADELNCRLVFDIIYRPQTTRLLQI
                     AARRGIAAVSGVEMFVAQGAAQWTIWTGEPAPVVAMRRAVLRVLSTADSPHRFSRRGR
                     RTSR"
     gene            1793..2671
                     /locus_tag="VN788_04775"
     CDS             1793..2671
                     /locus_tag="VN788_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19877.1"
                     /translation="MYGKTVLATLLLAVCAVAVAAQDQTSSLTPGVLYCSGVVSNEKV
                     PQDTYLITGEQSNSKLTFNQGDYVYFNKGTKQGAKVGDEFLVIRPVIDTVKIHWTKWQ
                     ASILHKMGTVWADEGRLKVIVARSDVSIAQVEDTCDPMQRGDYVVPFTERPLPPLKPA
                     NNFDRFAPSNGKPLATIITGKRFQQEVGTQDIVYVNLGSQQGVKVGDYFRIFRYQGTE
                     HETAYIPHRTAFDVEGVQGPTWGFGRAPSKYNWSNVPREGVGEGIVLRTGPNSSTVLI
                     TFVLREAFPGDYVEIE"
     gene            complement(2653..3264)
                     /gene="amrA"
                     /locus_tag="VN788_04780"
     CDS             complement(2653..3264)
                     /gene="amrA"
                     /locus_tag="VN788_04780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HXU19878.1"
                     /translation="MRSLDSPEKRSLLNLARGAVMTAVEGTDPVANPSCDVASAGFTG
                     AFVTLRYRSQLRGCIGEINSKEDLVTVIPRCARAAALDDPRFRPVRRDELAEIKIDIS
                     ILSPPRESKAEEIEPGKHGVIVSRGSRRGVLLPQVAMERGWGAQRFLEEACVKAGLER
                     EAWKTRGVMIETFTAETFGETDSQIDGGRNPPDRLKAGYSIST"
     gene            complement(3248..3880)
                     /gene="pgsA"
                     /locus_tag="VN788_04785"
     CDS             complement(3248..3880)
                     /gene="pgsA"
                     /locus_tag="VN788_04785"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HXU19879.1"
                     /translation="MNLPNSLTILRIFFVPLLVVVLLTRQPNWNFWGLPIHFEVWGVL
                     ILLVAAATDAMDGYFARSRREITTLGILLDPIADKLLISAAFISLVSMGLVQAWMVVI
                     IIGREFIVVGLREIASVEGLVIPASPLGKTKMVLQVLAGCAVILTANHPALKTVGIVL
                     LWLVVISAVLSAMHYFQMFWSQINFRVQERRRSSVVLVESKEKPQDALTR"
     gene            complement(4331..>4692)
                     /locus_tag="VN788_04790"
     CDS             complement(4331..>4692)
                     /locus_tag="VN788_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXU19880.1"
                     /translation="AQNGVRVNWTASSDAGGNPSLTYNVYRAGSCLGQLTKVNSAPLS
                     GTSYLDKDVATGAIYCYHVTAALNGIESPPSNQVIVAVPPPESRQGVCRHRGALIGWI
                     RCQITQAKKLDRTLSSH"
BASE COUNT         1054 a         1340 c         1246 g         1052 t
ORIGIN      
        1 taaaccggta tgagatttcc ctgctgtgcc aagcagcgga aagttttgaa atcgggttgg
       61 atcatctcga agttcgcctg ccacggcggc taaaacgatg cgggctatcc gcagtggaga
      121 gcacgcggag aaccgcacgg cgcatggcaa ccaccggggc tggctcgccg gtccaaattg
      181 tccattgcgc cgcgccctgt gccacaaaca tttccacgcc ggaaacggct gctatgcctc
      241 gacgcgccgc tatttggaga agcctcgtgg tttgcggacg ataaataatg tcaaatacca
      301 gcctgcaatt caattcgtcg gcctccaatg gcgaagtcga cacatcagga tacattccga
      361 cgggcgtggt gttcacgatt acgtcaaagg ctgtgtggcg gagggcggtt cttgcgattg
      421 cttcgccgcc cgctgcccgc gcgagatgtt tcgctctttt atatcgccgg gcgctcacga
      481 aaaccgccgc gccagcctgg gcaagcgcga aggctaccga gcgcgccgcc cctccggcgc
      541 ccacgatcaa tactcggctg ccgtgaagct tggtccgccg ctctagggtc cgcaatatac
      601 ccacgtaatc cgtattgtag ccatatagtt ttcctccgtt tcgcaccgca acggtgttca
      661 cggccccgat tttctcggca aggggatcac atcggtggag gaatcgcaaa atgcgctcct
      721 taagaggcag tgtcacgctg aaacctttga tgcccaaggg ttcgatcgaa tcaacaaagt
      781 ctcgcaggtc gtgtgtgagg aatggcaagt acacagcgtt catccgtcgc gcggagaagc
      841 cggcattctg catcgcaggg gaaagcgagt gggtgatcgg atcgccgacc acgccgtaga
      901 cgcgagttct ggcagtgact tgatccgctc gatagatcat tttcatttcg tcgagcgaaa
      961 tctggcccgg cgctgtcgaa ttttccacgg gcgcatacgc aaagccgttc gttccgcgaa
     1021 gcgccaaaag tcgagccggc tgtgccacat cacccatcgg cactgcgatc gtgttccgcc
     1081 gcttctcggc gaatcccagt agccgccggc tttcagccag ggagttacat ttcatagcga
     1141 tcttgatcgc atcggattgg ttacgtgcca tctcggcggc gactgccgtc aaatcttttg
     1201 gcggttcata aaaaaaatgc gccgatgtca gctgtcgacc ctggcccagg aggccgctga
     1261 gcaacgcctg tgagcactta cgcaccgttt caatctctag gtcgtaccac acgcagccag
     1321 ctcgaatagc ttgggccaaa tgtgctaact gattggaaat ggttccgcgg tagcgtcccc
     1381 ccgcctctct tcgacggcat gtggcgatca ggattgcgcg gggcctacta gtggctaggc
     1441 ggcgaaggaa aatgctgatc tcgcggtcgt ccgaaagcca gtccaaacgg agttctgcca
     1501 tgcgcgtaag gcgcaaagct ttggtgagct gcttgcccat ggcttccgca tcaggggcag
     1561 ctatcactgc acagatttta ttcagcccta aaagcttcat tttcaatcag ttcggcaata
     1621 gtacagcgag cctctagccg gtcacgacgg ggagacttcg cttctaccat gccgtgcaca
     1681 atgtgtaaag tccaaccgac cattaccttg acgcccgctg aatccgtctg ctacgttcga
     1741 tttaccgaga gaagccactt tcagcgccgg attcgataag gagcgagcct caatgtacgg
     1801 aaaaactgtt cttgcgaccc tattgttggc agtttgcgcg gtagctgtgg cggcgcagga
     1861 tcaaacatcc tcgctaacgc cgggtgtttt atattgttcc ggcgtcgtct cgaatgaaaa
     1921 ggtgccgcag gacacgtacc tcattaccgg ggaacagtcg aactcgaaac tgaccttcaa
     1981 ccaaggcgat tacgtctact tcaacaaggg tactaagcaa ggtgcaaagg tgggcgacga
     2041 gtttctggta attcggcctg tcattgacac agtcaaaatc cactggacca aatggcaagc
     2101 ttccattctt cacaaaatgg gtaccgtatg ggcagatgag ggccgcctca aagtgatcgt
     2161 tgcgcggtcc gatgtctcta tcgcgcaggt ggaggacact tgcgacccaa tgcagcgtgg
     2221 cgactatgtc gttccgttta ctgagcggcc cttgcctccg ctgaaaccgg ccaataactt
     2281 tgatcgattt gctccctcga atgggaaacc gctagcgacg attattacgg ggaagaggtt
     2341 ccagcaggag gtcggcaccc aagatatcgt ctatgtaaat ctgggcagcc agcaaggcgt
     2401 aaaggtcgga gactatttcc gaatctttcg ataccaggga actgaacatg agactgccta
     2461 catcccgcat cgtaccgcct ttgatgtcga gggtgtccag ggacccacgt ggggcttcgg
     2521 gcgagcgcca tcgaagtaca attggagcaa tgtacctcga gagggcgtgg gcgaagggat
     2581 cgtgctgcgg accggaccga attcgtccac cgttttgatc actttcgtct tgcgcgaggc
     2641 ctttccaggc gattacgtag agatcgagta gccagctttc aagcgatcgg gcggattacg
     2701 ccctccgtcg atctgcgagt cggtttcgcc gaaggtttca gcagtgaagg tttcgatcat
     2761 aactcccctg gttttccagg cttcgcgctc caacccagct ttaacgcacg cctcttctaa
     2821 aaaacgttgg gctccccagc cgcgctccat cgcgacctgg ggaagcagga cgccgcgcct
     2881 gctgcctcga ctcacaatca ctccgtgctt tcccggctca atctcttccg ctttgctttc
     2941 tctcggtggc gaaagaattg aaatgtcgat cttgatctct gccagttcgt ctcgtcgaac
     3001 tggcctgaag cgcggatcat ccagtgccgc ggctcgagcg cagcgaggta tcacagtaac
     3061 gagatcctcc tttgaattaa tctcgccgat gcatccgcgc agctgactgc ggtaccgtag
     3121 cgtgacaaaa gctccagtga atccggcgga agccacatcg caagacgggt ttgcgactgg
     3181 gtcggtgcct tcaactgccg tcatcactgc accgcgggcc agattcaaaa gcgaccgttt
     3241 ttccgggcta tcgagtgagc gcatcctggg gcttttcctt cgactcgacg agcacaacgc
     3301 tacttcgccg cctttcttga acccggaaat taatctggct ccagaacatc tggaaataat
     3361 gcatggctga gagcacggcg cttatcacca ccagccataa gagaactatg ccgaccgtct
     3421 tcagtgcagg atgatttgcc gtcaaaatga cggcgcatcc ggctagtacc tgcagaacca
     3481 ttttcgtttt tcccagcggc gaagctggaa taaccagacc ttcgacagaa gcaatttccc
     3541 gcaatcccac aacaatgaat tcgcggccga tgatgatcac taccatccaa gcttgcacca
     3601 gccccattga gaccagtgat ataaatgcag ccgaaattag cagtttatcc gcgatcggat
     3661 cgagcaaaat ccccaaagtt gtaatttcac gtctactccg cgcgaagtag ccgtccatgg
     3721 catccgtcgc agccgccacc agcagaataa gtactcccca aacctcaaaa tggatcggca
     3781 acccccagaa gttccagttc ggctggcgag tcagcagaac aacaaccaga agaggaacaa
     3841 agaagatccg aagaatcgtc agactattcg gcaggttcat ttcattctcg gggtcaaagc
     3901 aatcggctcc gtcaagtgct tggcacttaa tcggctaact gaatatagtc cccgcttccg
     3961 gttgagtcaa cgattcaagg ctagcaaacc ggttccaccg ttgggaaagc gagtgctcca
     4021 tctccaggca caactcctga tctagtagcc ccttacatgc gaaaatgcca catttctgtg
     4081 caaattgtct cgaagggcct cttctcaagg acgccgggcg cgacaggtcc gtactttctc
     4141 ataactcacg tgctatgaat ggcctgcacg aataagctag ggctagaaga ctcaggcgct
     4201 ggagttttaa tcagctttct gagtcttcct ttgcctccgt tttccacaag gttttccaca
     4261 cacgtgccct atggccgaag cgtaaatggt gtccccgtcc ggcctttttc agctactagg
     4321 tacgggtcgt ttagtgagat gacaatgttc ggtccagctt cttagcttga gtgatttgac
     4381 atcgaatcca cccgataagc gcgccccgat gccggcaaac gccctgtcgg ctttcaggtg
     4441 gagggaccgc cacaattact tggttcgagg gtgggctttc aataccgttg agggcagccg
     4501 tcacgtgata gcagtagatg gctcctgtcg ccacatcctt atcaagatac gaggtacctg
     4561 ataggggcgc cgaattcact ttcgtaagtt ggccgagaca agaaccggct cgatacacat
     4621 tgtaagtcag tgaagggttt cctccggcgt ccgaacttgc cgtccagttc actcgtacgc
     4681 cattttgcgc tc
//
LOCUS       DATNDT010000148         7646 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2703167, whole genome shotgun sequence.
ACCESSION   DATNDT010000148 DATNDT010000000
VERSION     DATNDT010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7646
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703167"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..529
                     /locus_tag="VN788_04795"
     CDS             <1..529
                     /locus_tag="VN788_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU19881.1"
                     /translation="FGSMTVGENVALPLREHTQLDESTIEIMVRLKLDQVGLSGFEDY
                     MPAQLSGGMKKRAAIARALAMEPEILFFDEPSAGLDPIIAAGIDELILKLKKAFRITI
                     LVVTHELASAFLIADRMILLDKGKIVANGTSKALRESEEPRVRQFLDRVPEPEVAGEI
                     DYLQMLTGEGPFERR"
     gene            632..1606
                     /locus_tag="VN788_04800"
     CDS             632..1606
                     /locus_tag="VN788_04800"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HXU19882.1"
                     /translation="MESKREQVVVGIFVLVVAALLAVTVFLLSGTFGRGNVPYRTYFK
                     NAGGLSPGAEVRYAGGPAVGRVKEVKSDPQNPTRMEIDFTVQPTVPVKIDSIAEITSV
                     SPLGDNFLGIVPGTLAAPKAPSGSTLKSIEYTSFSDVTALIAQLGPNANELLGNLNDR
                     VVALRETIDRVNNLLDARNRANISASLADLHGMLDENRPAIHSTLNNVNASSAKLGPL
                     IDDFRKTSSQANEALAHLDAAVSENRADLHKAVSDLRKTLDSTVTLTSQLDRTLDANS
                     ENLDEIIDNLRHVTENLNSFTETIKTRPYTLIRSSGVKPRKPGEAPPK"
     gene            1623..2255
                     /locus_tag="VN788_04805"
     CDS             1623..2255
                     /locus_tag="VN788_04805"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HXU19883.1"
                     /translation="MTTRQVQQALLIILASGITAACAASRPVKYYVLDTGPAATPESS
                     VARFPIKLLVGRVTASHLYRDDRLVYGSGPVQLGTYEYQRWAEPPAGMIEDMLIRSLQ
                     STGAYRSVSGLRSNARGDYIVRGHLAALNEVEGPVLSARFSFQLELFDPKTSATIWSD
                     SYAHDEPVTGKKVSDVVEALDRNVSAGLAQLTSHLGQYFAAHPPEPAAAQ"
     gene            2609..3073
                     /locus_tag="VN788_04810"
     CDS             2609..3073
                     /locus_tag="VN788_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012971782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19884.1"
                     /translation="MSKPDGVEILLVEDNPADAELTLHALNKHNLANRIQHARDGEEA
                     LDFIFCRGAFSARSKEAKPRLILLDLKLPKIDGMQVLREIKSDLTTKPIPVIVLTSSK
                     EEQDLVSSYQLGVNSYIQKPVNFAEFQDVVRQLGMYWLLVNAAPPAVASPVR"
     gene            3101..5461
                     /locus_tag="VN788_04815"
     CDS             3101..5461
                     /locus_tag="VN788_04815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19885.1"
                     /translation="MNKVHRPTSTSGKPVRLLIADDDPNDLELCLRDLKKSGLDFQAD
                     SAFTREEFAQKLGAEPADVVVSDYRMKGWTALDALAMIQESWPGVPLILVSGTLGDEL
                     AVECIKKGVTDYVLKGQLARLPTAIRHAQESRALQEAELRALEALRESEEHYRTLVEN
                     APEAIVLLDIEQQKFVDSNGRALSLFGLTRDTLLRSGVRDVSAAKQIDGATAEAMERL
                     FSEPTLDGATAHFEWRYRNSQGEEHPAEVHLVRLPSTNRRLVRASIVDITERKRSEQA
                     LRESEARYRGLVDNATYGILWIEEHGGRLLDANPAVAAMLRYDSVDDLLSIQFTKTLY
                     CNPADQNAIVAEYRRLGRAHGTVEWKRKDGKVAAVRLSSRRATNPDGSACVEVIVEDV
                     TEHKALERQLVQAQKFEAIGQLAGGIAHDFNNMIGAILGWVDLGLDETDANSRLHRHF
                     EKVRHQAERAAALTRQLLAFARRQILEPRNVDLNQTTIETLSLLEKVIGSNIEIKANL
                     APNLAVARADPTQIEQVLMNLCINARDAMPDGGSLIIDTSDMTLDATFCSLQPLARPG
                     EYVMLAVTDSGTGMDAATLDRIFEPFFTTKEMGKGTGLGLATVYGIVRQHGGFLNVYS
                     EPGVGSTFRVYLPISTTATPVRDSAEDTGPVAGGTETILVVEDHEGLRQLAQETLAGL
                     GYNVLLAADGQQAVKEFQRCPETNLVLLDVVLPKLSGPEVYAKLQETHPGLPVIFATG
                     YSPDLALIQKVQQQGFPVLQKPYSSRSLARAVRKTLDSETRPVSSG"
     gene            complement(5498..6637)
                     /locus_tag="VN788_04820"
     CDS             complement(5498..6637)
                     /locus_tag="VN788_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXU19886.1"
                     /translation="MPSSATDNQRIPGRATAQSTLRYAARFQGRAAAGHFRDVQGGLV
                     LSSIGIGTYLGEPDRATDEGYTNAVVAAVRGGVNVLDSAINYRLQRSERSVGAALKIL
                     SSDEFPRDEVLVCTKAGFLTPDGAMPEDASEYFSREYVERGILRPEDIAAGCHCMAPG
                     YLSDQLDRSRRNLGIECVDVFYLHNPETQLSEVTTDEFRRRVREAFLFLESAVSAGKI
                     GAYGLATWNAFRDERNSPGYLSLAAMEAIAREAGGQDHHFHFVQLPLNLAMPEALLRP
                     NQVVEGKTMAMVQAGRALGITIVSSAALLQGQLARKLPPYIGAALGLKDHSAQALQFA
                     RSVPGVTTALVGMSSVEHVRTNLEVVAVEPAPRDQFLKLFEPQTC"
     gene            complement(6716..>7646)
                     /locus_tag="VN788_04825"
     CDS             complement(6716..>7646)
                     /locus_tag="VN788_04825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU19887.1"
                     /translation="GSVNAFDIPARQSFLVEMVGREDLMNAIALNSTMFNGARIIGPA
                     IAGILVASIGEGWCFFANSVSYIAVIAGLLMMRIPPKPAVTHHVSPFEHIAEGFRFVR
                     HTAPIRALLLLIGLVSLVAMPYTVLMPIFAARVLHGNAKTLGILMGATGVGAMCGALM
                     IASRRGVRGFGKLVPIACGSFGAFLIVFTFSRWWILSAILLVPVGFSVMTQMSCSNTL
                     VQSMVPDRLRGRTMAVYSMMFLGMAPFGALIEGAAAGHIGAPLTVAIGGVLTIAGAIA
                     FARYLPKIRVQAREMIDAQGLGEPVEEIAARVS"
BASE COUNT         1714 a         2235 c         2208 g         1489 t
ORIGIN      
        1 attcgggtcg atgaccgtgg gagagaatgt cgcccttccc ttgcgcgagc atacacagct
       61 cgatgagtcc accatcgaaa taatggtgcg cttaaagctc gaccaagtgg gactttcggg
      121 cttcgaagac tacatgcctg cgcaattgag cggagggatg aaaaagcgcg cggcaattgc
      181 ccgcgcgctg gccatggaac ccgaaatttt gtttttcgat gagccctcgg caggtctcga
      241 ccccattatc gcggcgggaa tcgatgaact gatcctgaaa ttgaagaagg cattccgaat
      301 taccatcctg gtcgtaacgc acgaactggc cagcgcattc ctgattgccg atcgcatgat
      361 cctgctcgat aagggaaaga tcgtcgccaa cggaacgtcg aaggcgcttc gcgaaagtga
      421 ggagccacgc gtgcggcaat ttttagaccg cgtccccgag cccgaggtgg ccggcgaaat
      481 cgattacctg cagatgctga cgggagaagg ccctttcgag cggcgctgaa actggcgaat
      541 cgcgcgccgc atgttatact cgccggaatt ctgcaggcgg ccagcccgac ttgctggggc
      601 gtgaccgggg ctggaaacta tagaggaacc agtggaatcg aagcgggagc aggttgtcgt
      661 tgggatattc gttttagtgg tcgccgcctt gctcgccgtg acggtttttc ttctttcggg
      721 aacattcggc cgcggaaacg tcccctaccg aacttatttc aagaacgcgg gcggactgag
      781 ccccggcgcg gaagtccgct atgccggcgg gccggcggtt ggtcgcgtga aagaagtaaa
      841 gagcgacccg cagaatccga cgcggatgga aatcgatttt accgtgcagc ccacagtccc
      901 agtaaagatc gacagcatcg ccgaaattac gagcgtcagt ccgctgggcg acaattttct
      961 tggaatcgtt cccggcacac tcgccgcgcc gaaagcgccg agcggctcca cgctcaagtc
     1021 aattgaatac accagctttt ccgacgtcac cgcgctcatc gctcaactgg gtccgaacgc
     1081 gaatgaactt ctggggaatt tgaacgatcg cgtggtggcc ttgagggaga caatcgatcg
     1141 cgtgaacaac ctgctggatg cgcggaaccg cgccaacatc tccgcgagtc tcgcggacct
     1201 tcacgggatg ctcgatgaga accgtccggc gattcattcg acgctgaata acgtgaatgc
     1261 atcgagcgca aaactcgggc ctctgatcga tgacttccgc aaaacttcct cacaagccaa
     1321 cgaggcgctc gctcatctgg atgccgcggt ctccgagaac cgcgccgatc tccacaaggc
     1381 ggtgagcgat cttcgcaaga cgctagattc aacggtaacg ctcacgagcc agctcgatag
     1441 gacgctggac gcaaattctg aaaatcttga tgaaattatc gataatcttc gccacgtcac
     1501 tgagaattta aattccttca ccgagacgat caagacgcgg ccttacacgt taattcgctc
     1561 gtccggggtc aagccgcgaa agcctggcga agcaccgccc aagtagagag gggagacgaa
     1621 gagtgacaac gcgccaggtg caacaagcct tgctgattat cctggcctcg ggaattaccg
     1681 cggcgtgcgc cgcttcacgg cccgtgaaat attacgtgct ggatacgggt cctgcagcga
     1741 cgcccgagtc ctcggtggcg cgctttccca taaagctcct cgtgggccgc gttaccgcct
     1801 cgcatctgta ccgcgacgac cggcttgtct acgggagcgg gcctgttcag cttggcactt
     1861 acgagtatca gcgttgggcg gagccgccag ccggtatgat cgaggatatg ctcattcgct
     1921 ctttgcagtc gacgggagcg tatcgttcgg tatccggcct ccgcagtaac gcgcgcggcg
     1981 actatattgt ccgcggccat ctcgccgccc ttaacgaagt cgaaggaccg gtcctctccg
     2041 cgcgcttttc ctttcaactc gaattgtttg accccaaaac cagcgccacg atctggtccg
     2101 attcctacgc gcacgatgaa ccggtgactg gaaagaaagt ttctgatgtt gtggaagcgc
     2161 tggaccgcaa cgtgagcgcc ggcttagcgc aactaacctc acacctcggc caatacttcg
     2221 cagcacatcc tcctgaaccg gcagctgcgc aataactgag tttagtattt tctaatcttc
     2281 cttccaattt cctggggctc gctcgatagt cgtacacccg gtgattcaag catagatcca
     2341 cttggcctga cacccgctga cgatatttgt ccgcgaatgc ggctggccct tcgacccgga
     2401 ttcgccgata agttgcttac gggctgcaag cggcggcggc gcggacatgg ctcccaagcc
     2461 atggttctgg gaggccacgc ttcggcgaac attcggcggc gcagcggcag gtatggacag
     2521 aatccgaccg gccaggtaac aacattctat tttgcgctgg aaccgcgttt gcagggtgcc
     2581 acatgggtac cttaaagggg ggcgagctgt gagcaagcct gacggagtgg aaatactgct
     2641 agtggaagac aatcccgctg atgcggagct gaccttgcac gctttgaaca aacacaatct
     2701 tgccaaccgc attcagcacg cccgggacgg ggaagaagcc ctggatttca ttttctgccg
     2761 cggagccttt tcggcgcgca gcaaagaggc gaaacctcgg ttgatactgc tggatttgaa
     2821 actgccaaaa atcgatggca tgcaggtgtt gcgagagatc aaaagcgatc tcaccaccaa
     2881 gccgattccc gtgatcgtgc tcacctcatc gaaggaagaa caggatctag tgagtagtta
     2941 ccagttgggc gttaacagct acattcaaaa gccggtgaac tttgccgaat ttcaagatgt
     3001 ggttcggcaa ctcggtatgt actggctttt ggtgaacgcc gcgcccccag cagtagcatc
     3061 gcctgtccgc tagcgccgcc aaacggatat gcaaacgaga atgaataaag tccacaggcc
     3121 gacatcaacg tccggcaagc ccgtgcgtct gctcatagcc gacgatgatc cgaacgatct
     3181 ggaactatgc cttcgcgatc tgaagaagtc aggcctggat tttcaggctg actccgcctt
     3241 cacccgggaa gaattcgcgc agaagctggg cgcggagccc gccgacgttg tggtctccga
     3301 ttaccgcatg aagggttgga cagcgttgga tgcgctggcc atgatccagg agtcttggcc
     3361 tggcgtaccc ctgattttgg tgagcggtac gctcggcgat gagcttgcgg tggaatgcat
     3421 aaagaagggc gtcaccgact acgtgttgaa ggggcagctt gcgaggctgc cgacggccat
     3481 ccggcacgcg caagagagtc gagcactgca agaagccgag cttcgggcgc tcgaggcgct
     3541 gcgcgagagt gaggagcatt accggacact cgttgaaaat gctccggaag caatcgtgct
     3601 gctcgatatt gagcagcaga aattcgtaga ttccaatggc cgcgcgctat cgctctttgg
     3661 tttaacgagg gacactcttc tgcgcagcgg agtgagagac gtaagcgccg ccaaacagat
     3721 tgatggggca acggctgagg cgatggaacg gttgttctca gaaccgacat tggatggagc
     3781 caccgcccac ttcgagtgga ggtaccgcaa ctcacaggga gaggaacacc cagccgaagt
     3841 gcatttggtt cgcttgccat cgacaaaccg acgcctggtg cgtgccagca tcgtagatat
     3901 caccgagcgc aaacggtcgg agcaagcgtt gcgggagagc gaggcccgct atcgcggact
     3961 ggtcgacaat gccacgtatg gaatcctctg gatcgaggag catgggggca gactgttgga
     4021 cgcgaatccg gcagttgcgg caatgcttcg ctacgactcg gtagatgatt tgttgagcat
     4081 tcagtttaca aagacgcttt actgcaatcc cgccgaccag aatgcgatcg tcgctgaata
     4141 ccgacggctg gggcgcgcgc acggcaccgt tgaatggaag cgtaaagatg gaaaggtagc
     4201 ggccgttcgc ctgagcagcc gtcgcgcgac caacccggac ggatccgcgt gcgtcgaggt
     4261 tatcgtcgag gacgtaacag agcacaaagc tctcgaaaga cagctcgtgc aggcgcagaa
     4321 attcgaagcg atcgggcagc ttgccggcgg aatcgcgcac gactttaaca acatgattgg
     4381 cgcgattctc ggctgggttg atctgggact tgacgaaacc gacgcgaatt cacggctgca
     4441 ccggcatttc gagaaggtac gccaccaggc cgagcgcgcc gctgcattga cgcgccaatt
     4501 gctggccttc gcccgccggc agatactcga gccgcgcaac gtagacctga atcaaaccac
     4561 gatcgagact cttagcctgc tggaaaaagt gattggcagc aacatcgaga tcaaggccaa
     4621 tctcgcgccc aatcttgccg tggcacgagc ggatccgact cagatcgagc aggtgctgat
     4681 gaatctgtgc atcaacgccc gggatgccat gcccgacggt ggctcgctga ttatcgacac
     4741 ctccgacatg acgctggatg cgacgttctg ttcgcttcag ccgcttgctc gccccggcga
     4801 atatgtgatg cttgccgtca ccgattcggg cacgggaatg gacgcagcca cactcgaccg
     4861 aattttcgag ccgttcttca cgaccaaaga gatggggaag ggaacgggac tcggactcgc
     4921 tacggtctac gggattgtcc ggcagcatgg tggctttttg aatgtatata gtgagccggg
     4981 tgtcggcagc acatttagag tttatctgcc gataagcacc acggccacgc ctgtgcgcga
     5041 ttcggccgag gacacagggc cggttgccgg tgggacagag acgatcctcg ttgtcgagga
     5101 tcacgaagga ttgcgccagc tagcccagga aactctcgcc ggcttggggt acaacgttct
     5161 tctggcggcg gatggtcaac aggcagtgaa agagttccag cgatgcccgg aaacgaatct
     5221 tgtgctcttg gatgtggtgt tgccgaaatt aagcggcccc gaagtttacg cgaagctaca
     5281 ggaaacgcat ccgggtcttc ccgtaatctt cgcaaccggt tatagccccg atttggcctt
     5341 gatccaaaag gtgcagcagc aaggctttcc cgtgttgcaa aaaccctact cctcgcgaag
     5401 tctggcgcgc gcagtgcgaa aaacgttgga tagcgaaacg cgtccggtct caagtggcta
     5461 aagttttaga ttgaggattc agcatgccgc ggcatcccta gcaagtttgt ggctcgaata
     5521 atttaaggaa ctgatcgcgg ggagcgggtt caaccgctac aacttccaga ttggtgcgca
     5581 catgctccac actgctcatt cccaccagcg cggtagtgac tccgggaacc gaccgtgcaa
     5641 actgcagcgc ctgcgccgag tgatccttca gcccaagcgc ggcaccaatg taaggaggca
     5701 gcttccgcgc gagctgtccc tgaagaagag cggcgctcga tacgattgtg attccaagcg
     5761 cacggcctgc ctgtaccatg gccatggtct ttccttcaac cacttggttc ggccgcagca
     5821 atgcctcagg catcgcaagg tttagcggta gctgcacgaa atggaaatga tggtcctgac
     5881 cgccggcctc gcgggcgatt gcttccatgg ccgctagcga gagatatccc ggcgagttgc
     5941 gctcgtcgcg gaaggcgttc cacgtggcca gcccgtacgc gccgattttc cccgcggaga
     6001 ccgcggattc caggaataga aacgcttcgc ggacgcggcg gcggaattcg tcggttgtca
     6061 cctcgcttaa ttgcgtctcc ggattgtgca aatagaaaac gtcgacgcat tcgatgccca
     6121 gattcctgcg gctgcgatcc aattggtccg aaagatagcc gggagccatg cagtggcagc
     6181 cagccgcgat atcttccggc cgcaagattc cgcgttccac atactcacgc gaaaaatatt
     6241 cgctggcgtc ttccggcatt gccccatcgg gagtcaggaa gcccgccttg gtgcagacaa
     6301 ggacctcgtc ccggggaaat tcatccgagc tcaagatttt caaggccgcg cccaccgagc
     6361 gctcgctgcg ctgcaaccgg tagttgatcg ccgaatcgag cacgttcacg ccgccccgca
     6421 cagcagcaac gaccgcgttc gtgtatccct catcggtcgc acgatcgggt tctccgagat
     6481 acgtcccgat cccgatcgag gaaagcacca agccgccctg cacgtcgcgg aaatgcccgg
     6541 cagcggcgcg tccttgaaac ctagctgcat agcgcaaagt gctctgtgcg gtggcgcgac
     6601 ccgggatgcg ttggttgtct gtcgcggaag acggcattgc aaccctcact tcaatgagcg
     6661 cgttggaaaa tccccgcaag ttgacgcaag tcgccgcggc actgtctctg aatcctcatg
     6721 aaacacgcgc cgctatttct tccaccggct cgcctagtcc ctgcgcatcg atcatctctc
     6781 ttgcctgcac tcgaatcttc ggaaggtatc gcgcgaatgc gatcgctccc gcaattgtca
     6841 ggaccccgcc gatggccacc gtcaaaggag cgccgatgtg accggccgcc gctccctcaa
     6901 tcagcgctcc gaagggcgcc atgcccaaga acatcatcga atacacggcc atggtgcggc
     6961 cccgcagccg gtccggcacc atcgattgaa cgagtgtgtt cgagcacgac atctgcgtca
     7021 tcaccgaaaa accgaccggc accagcagga tcgctgagag aatccaccag cgcgaaaatg
     7081 tgaacacgat caaaaaagct ccgaacgagc cgcaagcgat cgggactaat tttccaaaac
     7141 cgcgaacccc cctgcgcgac gcaatcatca gcgcgccgca catcgctccc acgccggtgg
     7201 cccccatcaa tatacccagc gtcttggcat taccgtgcag cactctcgcc gcaaagatag
     7261 gcatcaatac cgtatacggc atggcgacca ggctcaccaa gccgattagc aggagcagcg
     7321 cgcgaatcgg cgccgtgtgg cgcacgaagc gaaatccttc ggcaatgtgc tcgaagggag
     7381 acacatgatg cgttactgcc ggtttcggtg ggatgcgcat catcagcaat ccggcgatca
     7441 cggcgatata acttaccgaa ttcgcgaaaa agcaccaacc ctcgccaatg ctggccacca
     7501 gaatccccgc aatcgcaggc ccgataatgc gcgccccatt gaacatcgtg gagttcaaag
     7561 cgatcgcgtt catcagatct tcccggccaa ccatttccac caaaaatgac tggcgagccg
     7621 gaatgtcgaa tgcgttcacg ctaccc
//
LOCUS       DATNDT010000149         4893 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_340231, whole genome shotgun sequence.
ACCESSION   DATNDT010000149 DATNDT010000000
VERSION     DATNDT010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4893
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340231"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1998
                     /gene="pheT"
                     /locus_tag="VN788_04830"
     CDS             <1..1998
                     /gene="pheT"
                     /locus_tag="VN788_04830"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXU19888.1"
                     /translation="DLTSAGITVEGVSGEGANTVFEMEITTNRPDAMNHYGVARECSA
                     IYDLPLKSLETELPASTGEDARASIEIQDKQGCARYTARVIHGVKIKASPEAIVNRLA
                     SVDQRAINNAADASNYTLWEMGHPTHAFDLDLLEGKKIIVRRATKGETLKTLDGVERK
                     LYVDDLIIADAKKPVALAGVMGGFDSMITEKTENILIESAWFDPTVVRKTAKRHGLHT
                     DASHRFERGADYGATSIACARVAMRILESGGGELQGNEIDAVARVLDLAPIQLDRAEV
                     KRILGEDLGDTEIVRILRRLGFVVIPDTNSDYLVSVPSWRLDVEREIDLIEEIARLHG
                     YDKFRKTLPAFAGAVAESPDSRKDERLRSALLALGYNEAVSLSFISHADAEQFSSLPV
                     IELENPLSEEASVMRTSLVPGILNMLAYNLNRGNDDTRLFEAGSVFSTADGKPHEPKQ
                     ICLGATATNLEVQLPKGGILDVGKGESAIAAEVFRSFKGDIETLLDEFHHGAIHYDSQ
                     TSDYYHPSRSARALLNGEAIAQFGQLDPGVIAQRKLRHNIFIGEIYLDRLYNHELRKI
                     QYQPLPRYPAVERDFSFIFSDDVVFAKIQAAVSSLSLSEPREFSPAEIFRGGSIPPGK
                     YSVLLRATFQSYERTLRDEEVAEWSAKIVKALESLGGAQRA"
     gene            2315..3469
                     /locus_tag="VN788_04835"
     CDS             2315..3469
                     /locus_tag="VN788_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide isomerase"
                     /protein_id="HXU19889.1"
                     /translation="MPLVTYEQAKPFAPAIAASVQSKMMPPWFADPSIGHFADDPSLT
                     PQQIATILAWAKTGAPAGDPKAAPPAPHWTKGWNITHPDQVFEMPQPVPIPADGDIEY
                     TYEIVPTHFTEDRWVQMSEVRPSSPQYVHHAVVYIRPPNSNWLRHAPIGTPFTASTLQ
                     DPIERREAHETTNDLLLVYAPGSAPDQWPQGMAKLIPAGSDLVFQMHYTSNGHAGTDQ
                     TALGLVFAKQPPAQRVITLQLNNHALLIPPNADNFRVEVEGTLPYNATLLSFFPHMHL
                     RGKAFEYDIVHDDGTIEPLLRVNHYHFHWQLSYKLAQPRFLKAGTRLRAVAWYDNSRN
                     NPHNPDPDALVTWGDQTYQEMMVGFFDVAVPANVDKQKFFIRKPVSKQVN"
     gene            3808..>4893
                     /locus_tag="VN788_04840"
     CDS             3808..>4893
                     /locus_tag="VN788_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19890.1"
                     /translation="MTQARAMTRNLLIAIGLFALLLFFAGCGGNAAMSAGSASGGSGS
                     GGGPGSGGGSGSGGGSGGSGGTTTPNTSCAMTAGQGASLNGFIPFTSDNPWNQDISSA
                     TVDPNSSQIINFIGPTIGLHPDFGAGQFQGSTIGIPYTIVDGTQPTVTINFNQSADES
                     DPGPMPMPASAPIEGYPNPGNGDRHVLVLDNSNCFLYELYASSANSNGSWTAGSGAVW
                     DLLANEQRPWTWTSADAAGLPIFPGLVRYDEVAGGKIQHALRFTLNDSRAAVVPPASH
                     WAANSTNSLAAPMGMRLRLKASVDISSFSPNVQVILTALKKYGMIMADNGSSMFLSGA
                     PDDRWDNDDLHTLSQVPASAFEVVQMTP"
BASE COUNT         1088 a         1628 c         1219 g          958 t
ORIGIN      
        1 gacctgacct ccgccgggat caccgtcgaa ggcgtcagcg gcgaaggcgc gaacaccgtc
       61 ttcgaaatgg agatcaccac gaatcgaccc gacgcgatga atcactacgg cgtcgcgcgt
      121 gaatgctcag cgatctacga tctcccgctc aagtccctcg aaaccgagct gcccgccagc
      181 accggcgagg acgcccgcgc ctccatcgag attcaagaca agcaaggctg tgcacgctac
      241 accgcgcgcg tgattcacgg cgtgaagatc aaggcttcgc cggaagcaat cgtaaaccga
      301 ctggccagcg tggaccagcg cgccatcaac aacgccgcag acgcgtccaa ctacaccctc
      361 tgggaaatgg gacatcccac ccatgcattc gatctcgatc tactcgaagg caaaaagatc
      421 atcgtccgcc gcgccaccaa gggcgaaacg ctcaaaacgc tcgacggcgt agagcgcaaa
      481 ttgtacgtag acgacctcat catcgccgac gccaaaaagc cggtcgcgct cgccggagtc
      541 atgggcggct tcgattcgat gatcacggaa aagacagaaa atatcctgat cgagtcagcc
      601 tggtttgatc ctaccgttgt ccgcaaaacg gcgaagcgcc acggactgca caccgatgct
      661 tctcaccgtt tcgagcgcgg cgcggactac ggtgcaacct ccatcgcctg cgcgcgggtt
      721 gccatgcgca ttctggaatc cggaggcggc gaactccagg gaaatgaaat tgacgcagtc
      781 gcgcgcgttc tcgatctcgc tcccatccaa ctcgaccgtg cggaagtaaa gcgcattctc
      841 ggtgaagacc tcggcgatac ggaaatcgtt cgcatcctgc gccgcctcgg attcgtcgtc
      901 attcccgaca caaactccga ttacctggtg agtgttccca gttggcgcct cgacgtggaa
      961 cgcgaaattg acttaatcga agaaattgcc cgtctgcacg gctacgacaa attccgcaaa
     1021 acacttcccg ccttcgccgg ggccgttgcc gagtcccccg attcgcgcaa agacgaaaga
     1081 ctgcgctccg cacttctcgc cctcggctac aacgaagccg tctcgctgag ttttatctcc
     1141 cacgcagacg ccgagcaatt ttcctcgctt ccggtcatcg aacttgaaaa tccgttaagc
     1201 gaagaagcct cagtcatgcg gacatcgctc gtccccggca ttctcaatat gctcgcctac
     1261 aacctgaatc gtgggaacga tgacactcgg ctattcgagg ctggctcggt tttttcgacg
     1321 gcggatggaa agccgcacga gcccaaacag atttgccttg gcgcgactgc caccaacctc
     1381 gaagtacagc tgcccaaggg cggcattctc gatgtgggca aaggtgaaag cgcaatcgcg
     1441 gcagaggtct tccgcagctt caagggcgac atcgaaacct tgctggatga gttccatcac
     1501 ggcgcgatcc actacgattc gcaaacatcc gattactatc accccagccg ctccgcgcga
     1561 gctttgctga atggcgaggc aatcgcccag ttcggccaac tcgaccccgg cgtcatcgcg
     1621 caaagaaaac tccgccacaa tattttcatt ggcgagattt atttagatcg tctatacaac
     1681 cacgaactgc gcaaaattca gtaccagccc ttgccgcgct atccggcggt cgagcgtgat
     1741 ttctccttca tcttcagcga cgatgtcgtc ttcgcaaaaa ttcaagccgc ggtctcctca
     1801 ctcagtctca gcgaaccgcg tgaattctct cccgctgaaa tcttccgcgg cggctccatc
     1861 ccgccaggaa aatattccgt cctgctgcgc gcgaccttcc aatcttacga acgcaccctt
     1921 cgagacgaag aagtagccga gtggtctgcc aagatcgtga aagcgctcga gtctctcggt
     1981 ggagctcagc gagcttagcc ctaaacgaat cacgtgggga cagccgccct cggctgtccg
     2041 agctgacaag cgatgtaggc gcgggcgtcc cgcccgtgca atgtcgagcg aagctcgaca
     2101 ctcctattcc cgcaaatcca ctaagataaa cacatccgtg ccacgcctaa tctactcttc
     2161 gtttttcctc gcaattgtca ctgccatatt attttccatc gttcatccgc aaccgatttc
     2221 agcttcaaat ccaacgccaa ctttttacca agacatcctc cccatcctcc agaaccactg
     2281 ccaaagctgt caccgccccg gcgaagtagc gcccatgccg cttgtaacct acgagcaagc
     2341 caagccattt gccccagcaa tcgccgcctc cgtacaaagc aaaatgatgc cgccctggtt
     2401 cgccgacccc tccatcggac acttcgccga cgacccttcg ctgaccccgc agcagatcgc
     2461 cacaatcctg gcttgggcga aaacaggcgc gcccgccgga gacccgaaag ctgctccgcc
     2521 cgctcctcac tggacgaaag gctggaacat cacgcatccc gatcaagtct tcgagatgcc
     2581 gcagcccgtg ccgattcccg ccgacggaga catcgaatac acctacgaga tcgtacccac
     2641 gcatttcacc gaagaccgct gggtgcaaat gtcagaggtg cggccctcaa gcccgcagta
     2701 cgtacaccac gccgtcgtct atattcgccc gccaaattcc aattggcttc gccatgcgcc
     2761 aatcggcacg cccttcaccg cttccacttt gcaagatccc atcgagcgcc gcgaagcgca
     2821 tgaaactacc aatgatttgc tgctggtata cgcgccgggc agcgctcccg accagtggcc
     2881 gcaaggcatg gcgaaactta ttcccgccgg ctccgacctg gtttttcaaa tgcactacac
     2941 ttcgaatggc cacgcaggca cagaccaaac cgcgctaggg ctggttttcg ccaagcagcc
     3001 tcccgcgcag cgcgtgatca ctctgcaact aaacaatcac gcgctcctca tcccacccaa
     3061 cgccgacaat tttcgcgtgg aggtggaggg aacgctcccc tacaatgcaa ccctgctcag
     3121 ctttttcccg cacatgcacc ttcgcggcaa agcttttgaa tatgacattg ttcacgacga
     3181 cgggaccatc gagccgctct tgcgcgttaa tcattatcac ttccactggc agttgagtta
     3241 caagctggcc cagccgcgct ttctaaaagc cggcacgcga ttgcgcgccg tcgcctggta
     3301 tgacaactcc cgcaacaatc cccacaatcc cgaccccgac gcattggtga cctggggcga
     3361 tcagacttat caggaaatga tggttggctt tttcgacgtc gccgtccccg ccaacgtgga
     3421 caagcaaaag ttcttcatcc gcaaacccgt ctcgaagcaa gtgaactagc atggggctta
     3481 gaggatatgt gattcactct gcgaacctcg cgcgcagtaa gggaactccg atccactagc
     3541 gagattctga tgcaccgagc ggactccgat ttcacccgat cctcttcact aaatttcttg
     3601 tcatttcgag cgcaaccaaa aagtgttttt ttgctttttt gggaagcgag aaatctgctg
     3661 ttagcgtaaa tcagcacgga ctgagcacct cctacttctt cgcctgaact actggctctg
     3721 tcattccgcg gtacatgtcc ctattctgga tgcgtactga cctctttagc tgtgaacatc
     3781 tccgcatttt cccggagaat aggagccatg acgcaagcta gagccatgac cagaaatctg
     3841 ctcatcgcaa tcggactatt cgccctgctg ctgttcttcg ccggctgcgg cggaaacgct
     3901 gccatgtccg caggaagcgc atctggcgga agcggctctg gaggcggccc aggcagcgga
     3961 ggaggctctg gctccggcgg cggttccggc ggaagcggcg gcactacaac tccgaatact
     4021 tcctgcgcca tgaccgccgg tcaaggcgcg agcctcaacg gattcatccc cttcaccagc
     4081 gacaacccct ggaaccagga catttccagc gcgaccgttg atccgaactc cagccagatc
     4141 atcaatttca tcggccccac gatcggcctg catcctgact tcggtgcggg ccagtttcaa
     4201 ggctcgacta tcggcattcc ctacaccatc gtggacggca cacagcccac ggtcaccatc
     4261 aacttcaatc agtccgccga cgagagcgat ccaggcccca tgcccatgcc cgcaagcgcg
     4321 cccatcgagg gctatccgaa tccgggcaac ggtgaccgcc acgtgctcgt gctcgacaac
     4381 agcaattgtt ttctctacga gctgtacgcc tcttcggcga atagcaacgg aagctggacg
     4441 gctggctccg gcgcggtgtg ggatttgctg gcaaatgaac agcgcccctg gacgtggacc
     4501 tctgccgacg ccgccgggct cccgattttc cctggcctag ttcgctatga cgaagtcgcc
     4561 ggcggaaaaa tccagcacgc cttgcgcttc accctgaacg acagccgcgc agcagtcgtt
     4621 cctcccgcat cgcactgggc cgccaactcc accaactctt tggccgcgcc catgggcatg
     4681 cgcctgcgtc tgaaggccag cgtcgacatt tcgtcgttct cgcccaacgt gcaggtaatt
     4741 ctcaccgcgc tgaaaaaata cggaatgatc atggccgaca acggcagctc catgttcctg
     4801 agcggcgcgc ccgacgatcg ctgggacaac gacgacctgc acacgctctc gcaggttcca
     4861 gcatcagctt ttgaagttgt gcagatgacc ccg
//
LOCUS       DATNDT010000150         6164 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1756062, whole genome shotgun sequence.
ACCESSION   DATNDT010000150 DATNDT010000000
VERSION     DATNDT010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6164
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1756062"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..646)
                     /locus_tag="VN788_04845"
     CDS             complement(137..646)
                     /locus_tag="VN788_04845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010587962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU19891.1"
                     /translation="MALRDTFLPEFEYEMTTTRKTLERVPEDKVDWKPHDTSMAMGRL
                     AGHIAEMCGFAAATFQGDSFDFAPPGGTPTQPTVMKSRQQLLDIFDKNVASSRAAISK
                     ASDEDLQKTWTLLSGGKTFFAMPRIQVLRSMILNHVIHHRGQLSVYLRMNQVPVPSIY
                     GPSGDEGRM"
     gene            complement(875..1558)
                     /locus_tag="VN788_04850"
     CDS             complement(875..1558)
                     /locus_tag="VN788_04850"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXU19892.1"
                     /translation="MLTRTIGRYSSWPIDHLAALLAATGIPPNVITWAALFGNFWAGV
                     LFAEGRFAAAGGVMILAGLCDLLDGPVARRQNRISLFGGFLNSIVDRYADLILFLGLL
                     VYYAQVNRFRYAVLAGAAMAGAVMVSYAKARAESLVPRAEVGFWDRPERLVLMILGAL
                     SNRMEIALWILAIGPNLTVIHRIVHTWRHTKAPAPEPPPTVAHESNHDEMHEAPVLSR
                     SHSAGRGRA"
     gene            complement(1576..2076)
                     /gene="greA"
                     /locus_tag="VN788_04855"
     CDS             complement(1576..2076)
                     /gene="greA"
                     /locus_tag="VN788_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HXU19893.1"
                     /translation="MAPTNIHMPDIADKIKKKLQDEIAVLEHELHSDLPKEIMTARAH
                     GDLKENAEYKYAKERQSYVSARLGQLHQRLADISMLNLNNLPKDRAAYGSRILVLDVA
                     KSSKIEYKLVTVEEADAAKGLISTTSPIGRALLGKKVGDEVSVTTPAGIKEYEVVRLA
                     TIHDED"
     gene            complement(2209..2685)
                     /locus_tag="VN788_04860"
     CDS             complement(2209..2685)
                     /locus_tag="VN788_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXU19894.1"
                     /translation="MEPRLNPYKLSPKIYETMSALQAYVDSCGLERPLLELVKLRASQ
                     INGCAYCIDMHTKDARALGETEQRLYLLNAWRESPVYTERERAALEYTEAVTLVADTN
                     VPDDVYEIAARQFKPNELANLTLAIATINSWNRLSIAFRAVPGSYKSNLQRLEAKA"
     gene            2831..3976
                     /locus_tag="VN788_04865"
     CDS             2831..3976
                     /locus_tag="VN788_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HXU19895.1"
                     /translation="MNLELTEEQKLLQRSVRDFAEAEVKPLAKELDETGRFPHEIVRK
                     AAELGLTGISVLEKYGGAGMDHICYSIVMEEIARVCASTSVILSVQNSLYCATIDRFG
                     TEEQKQKFLVPFARGEKIGCYALTEPQAGSNAAALATKAVRKGDCYKVNGTKAWITNG
                     GVADAGVVYVNTQPEKGEKGITALIVEKGMPGFRVGKEEKKLGIHATACTELSFSDCE
                     VPAENRIGNEGEGYKIALSMLDGGRIGIASQATGIAQGAFEAALDYARQRQAFGHPIA
                     EFQAIQFMLADMATEIDAARLLARRAAWKQDSGSRFSMEAAIAKLFASEMATRVAHKA
                     IQIHGGNGYSREYPVERAYRDARITEIYEGTSEIQRLVIASWVLKGN"
     gene            3984..5189
                     /locus_tag="VN788_04870"
     CDS             3984..5189
                     /locus_tag="VN788_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19896.1"
                     /translation="MYSSPVLPLDSSEPFDSARDTEFFAALPSRPGVLLIEIREPGRP
                     YLTRTADIRRAAQRLLGVPEENSKRLNLREQAARVVYRATGSKFEQTLTLYEQARKFL
                     PDRYRDLLRLRPPAVLKINLRNAYPRCHVTRRIRADEGFYFGPFASRRSAEAFSESFL
                     DLFKIRRCQIKIRRDPNFSGCIYSEMKMCLAPCFAGCTREEYEAEVALVVETLDTAGG
                     ALTAKFEREREIASEALDFERAAALHKRLEKVAAVLRGLPELARRVGELDAVILQRGN
                     DEKTIVVFPLLGGVLAEAIFLNFAELSSQPRSVEAILRQALQPHPATASRGSGESSAI
                     NLRKRYGLHAAPEELPEHLSLVARWFYSKPRGGEILFREGDWPYRRILRACSRLLAPN
                     PSSPEPPAS"
     gene            5249..>6164
                     /locus_tag="VN788_04875"
     CDS             5249..>6164
                     /locus_tag="VN788_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU19897.1"
                     /translation="MAETSATGPAAARGLASRSRFHDTIRSLKHRNFQLFFSGQSISL
                     IGTWMDTIAEAWLVYRLTGSSLLLGTVAFAGQIPVFLLSPVGGMVADRFNRRKVVIAT
                     QVAFMIFAFILAMLTLTKRVTVMEVIILAALMGSVNAFDIPARQSFLVEMVGREDLMN
                     AIALNSTMFNGARIIGPAIAGILVASIGEGWCFFANSVSYIAVIAGLLMMRIPPKPVV
                     THHASPFEHIAEGFRFVRDTPPIRALLLLIGLVSLVAMPYTVLMPIFAARVLHGNART
                     LGILMGATGVGAMGGALMIASRRGVRGFG"
BASE COUNT         1259 a         1631 c         1970 g         1304 t
ORIGIN      
        1 gccaaaaccc tcaccccgtg acggcggggc tgagctacat gaaccggacg acgccgaaaa
       61 cccctcacgg cgatccgcga cagcaccttt ttcaaatcga ccgcaacaac tcagtacgct
      121 gacgaaatgc gggcggttac atgcggcctt cgtcgccaga gggaccgtag atagaaggta
      181 cagggacctg attcatccgc aaataaacgg agagctggcc gcgatggtgg atcacgtgat
      241 tcaaaatcat actgcgtagg acttggatgc gcggcatggc gaagaacgtc ttcccgccgc
      301 tcaatagggt ccaggttttc tgcaggtctt catcgctggc ctttgagatc gcggcgcgcg
      361 aggaggcgac gttcttgtcg aagatatcga gcaactgctg gcgggacttc atgaccgtcg
      421 gttgtgtggg agtccctccc ggcggtgcaa agtcgaagga gtcgccttgg aacgttgcgg
      481 ccgcgaagcc acacatctca gcgatgtgcc ccgccagccg gcccatcgcc atcgaggtgt
      541 catgcggctt ccaatcgact ttatcctcag gaacccgctc cagggttttt cgggtggtgg
      601 tcatttcgta ttcgaactcg ggaagaaatg tgtcgcgtaa tgccatgact gttgcctcct
      661 tagtttttcg gacggcgcga ttttacccca ggggtgaatg gcgaataccc aggcaatcct
      721 gcctggacaa gcagcaaatc taaaccagat tggatggccg ggtccttagc tgagattctg
      781 gatttatttc tccgcgagct cgagatttcg cgcatgttta tcaacctgcc cacttgaacc
      841 acgccttagg taccagatca ggcaggagta cggcttaggc gcgaccgcgg cctgccgaat
      901 gggagcgcga gaggacggga gcctcgtgca tttcgtcgtg gttgctctcg tgagcgacgg
      961 tcgggggcgg ctccggcgcg ggtgctttcg tgtgcctcca cgtgtgcacg atgcgatgga
     1021 tgacggtgag attggggccg atagccaata tccacagggc gatctccatg cgattggaga
     1081 gagcgccgag gatcatcagc accagacgct cgggccggtc ccaaaatcca acttcggcgc
     1141 gaggcaccag agattcggcg cgcgccttgg catagctgac cattaccgcg ccagccatgg
     1201 cggcgcccgc gagtaccgcg taccggaagc ggttcacctg ggcgtagtag acgagcaggc
     1261 caaggaacag gattaggtca gcatagcggt caacgatcga attgaggaaa ccgccaaaaa
     1321 gggaaatccg attttggcgg cgcgccaccg gtccatcgag gagatcgcag agccccgcca
     1381 gaatcatcac gccaccggcg gcagcgaagc gtccctcggc gaataggact ccggcccaaa
     1441 aattgccaaa cagggcggcc caggtgatta cgttgggagg gattcccgtt gcagccaaaa
     1501 gagccgcgag gtgatcaatg ggccaggaac tatagcggcc gatggtccgc gtcaacatga
     1561 agagatgtcg ctccgctaat cttcatcatg gatagtggcc aggcgtacaa cctcatattc
     1621 ttttatgcca gccggagtag tgacgcttac ttcgtcgccc acttttttcc cgaggagcgc
     1681 ccggccgatg ggcgaggtag tggaaatcag gcctttcgcc gcgtcggcct cttcaacggt
     1741 aaccagcttg tactcgattt tggaggattt cgcaacgtcc agaacgagga tgcgcgagcc
     1801 ataggccgcg cgatccttgg gaagattatt cagattgagc atggagatgt cggccaggcg
     1861 ttgatgaagc tggcccagcc gcgctgagac gtaggactgc ctctccttgg catacttata
     1921 ctcggcattt tccttgagat cgccatgggc gcgcgccgtc atgatctcct tggggagatc
     1981 gctatgcagt tcgtgctcga gaacggcgat ttcgtcctgc agcttctttt tgattttgtc
     2041 ggcgatgtca ggcatgtgga tgtttgtggg cgccacgacg ggcgtgcgcc gcagtcagta
     2101 tgggtttttc ttcctattgc caagtataga ggatttacgc gcgtagccgc aaatggacca
     2161 gtcgacacgc atccggcgcg tgcccagcgg cgttgactgg tggtggcctc aggctttggc
     2221 ttccaaccgc tgcaggttgg acttgtagga tcccggcacg gcgcgaaaag caatgctcag
     2281 cctgttccag ctattaatgg tggcaattgc caaggttaag ttggcgagtt cgttgggctt
     2341 aaactggcgc gcggcaatct catacacatc gtcgggcacg ttggtgtccg cgactagggt
     2401 gacggcctcg gtgtattcga gggccgctct ttcacgctcg gtgtacacgg ggctctctcg
     2461 ccaggcgttg agcaggtaaa ggcgctgctc tgtctcaccg agggcacgcg catccttggt
     2521 gtgcatgtcg atgcagtacg cgcagccgtt gatctgcgat gcgcggagct ttacgagttc
     2581 gagaagaggc cgttcgaggc cgcaggaatc gacataggcc tggagggcgg acatggtttc
     2641 atagattttt ggagagagtt tgtatggatt gaggcgtggt tccatcgtag acgctccttt
     2701 caatgtgcag tttcgagtat atcgctggag gaagagggac gaaacccgcc cgtggcgggt
     2761 tcgctgtaca attgcagatt gttaccggtc atccgaggac tcgaatgagg ggaagcggca
     2821 ggagaagctt gtgaatctgg aactcaccga agagcagaag cttttgcagc gaagcgtgcg
     2881 cgatttcgcg gaggcggaag tgaagccgct ggcgaaagag ctggacgaaa ccggccgctt
     2941 cccgcatgaa atcgtgcgca aagccgcgga actgggtctg acgggaattt cggtgctgga
     3001 aaaatacggc ggcgcgggaa tggaccacat ttgctattca atcgtgatgg aagaaattgc
     3061 gcgagtttgc gcgtcaacga gcgtgatcct ttcagtccag aactcgctgt actgtgcgac
     3121 gatcgaccgt tttggcaccg aagagcagaa gcagaagttc ctggtgccgt ttgcgcgagg
     3181 agagaagatc ggctgctacg cacttacgga gccgcaggct ggttcgaatg cggcggcgct
     3241 ggcaacgaaa gcagtgcgca aaggcgattg ctataaggtg aatggcacga aagcgtggat
     3301 cactaatggc ggcgtggcgg acgcgggggt cgtctatgtg aacacacagc cggaaaaagg
     3361 cgagaagggg atcacggcgc tgatcgtcga gaaaggcatg ccgggatttc gcgtcggcaa
     3421 agaggaaaag aagctgggca tccatgcgac ggcttgcacg gaactttcgt tctcggattg
     3481 cgaagtgccg gccgagaacc gcatcgggaa tgagggagaa ggctacaaga tcgcgctctc
     3541 gatgctcgat ggcggccgga tcggaatcgc gtcgcaagcg acaggcattg cgcagggcgc
     3601 ttttgaagcg gctctcgact acgcccggca gaggcaggcg ttcggccatc caatcgccga
     3661 gtttcaggcg atccagttca tgctggcgga catggccact gaaatcgacg ctgcacgatt
     3721 gctggcccgg cgcgccgcgt ggaaacaaga ttcgggctcg cggttttcaa tggaggcggc
     3781 tatcgcaaaa cttttcgcaa gcgagatggc cacgcgtgtg gcgcacaagg caattcagat
     3841 tcatggcggc aacgggtaca gccgggaata tccggtcgaa cgcgcctatc gcgatgccag
     3901 aatcacggag atttacgagg ggaccagcga gattcaaagg ctggtgatcg cctcgtgggt
     3961 tttgaaaggc aactagcttt ccgttgtatt cttccccggt gcttccgctc gatagttccg
     4021 agccattcga ctcggcgcgc gatacggagt tttttgcggc gctgccctcg cgacccggcg
     4081 tcttgctgat cgaaatacgc gagcccggac ggccctatct gacgcggacg gcggacattc
     4141 ggcgcgccgc gcagcgactt cttggcgtgc cggaagaaaa ctcgaagcgc ctcaacttgc
     4201 gggagcaggc tgcgcgagtc gtgtacagag ccaccggttc gaaattcgaa cagacgctga
     4261 cgctttacga gcaggcacgt aaattcttgc ccgatcgcta ccgagaccta ttgcggctgc
     4321 gcccgccggc ggtattgaag atcaatctgc gcaacgcgta cccgcgctgc catgtgacac
     4381 ggcgcatacg cgccgacgaa ggattctact tcgggccttt tgcttcgcgc cgatcggcgg
     4441 aggcgttttc ggaaagcttc ctcgatttgt tcaaaattcg gcgctgccag atcaagatcc
     4501 gccgcgatcc gaatttttcc gggtgcatct attcggaaat gaagatgtgc ttggcaccgt
     4561 gtttcgctgg ttgcaccagg gaagaatatg aggcggaggt ggcgctggtc gtcgaaacgc
     4621 tcgatacagc ggggggcgcc ctcacggcaa aatttgaacg cgagcgggaa atagcgagcg
     4681 aagctctgga tttcgaacga gccgctgcac tgcacaagcg gctggagaaa gtggcggccg
     4741 tgttgcgcgg gctgccggaa ctggcccggc gggtggggga actcgatgcg gtgatcctgc
     4801 agcgcgggaa cgatgaaaaa accattgtcg tgtttccgtt gctgggcgga gtgcttgcgg
     4861 aagcaatatt tcttaatttc gccgaactct cgagccagcc gcgttctgtg gaagccatct
     4921 tgcgtcaagc gctgcaaccg caccccgcta cggcttcgcg tggaagcggg gaatcgagtg
     4981 ccataaattt gcgaaaacgg tatggcttgc acgccgcgcc agaggaattg cccgagcatc
     5041 tatcgcttgt ggcgcgctgg ttttattcga agccgcgagg cggcgaaatt ctgtttcggg
     5101 aaggcgattg gccttatcgc cgcatcctgc gggcatgcag ccggcttctg gctcccaatc
     5161 cttcttcacc agaaccgccg gcttcctgaa gaaacgaggg cacaatcacg ggtcgtgcca
     5221 gcggcgttgc atcgtacaat tcaatattat ggcggaaacg agcgcaacgg ggcctgccgc
     5281 ggcccgcggg ctggccagcc gttcacgatt ccacgacacg atccggtctc tgaagcaccg
     5341 gaacttccaa ttatttttca gcgggcaatc gatttcgctg atcggcacgt ggatggacac
     5401 gattgccgag gcgtggctgg tttaccggct gacgggatcg tcactgctac tgggcacggt
     5461 ggcattcgcc gggcaaattc cagtttttct attgtcgcct gtgggtggga tggtggcgga
     5521 tcgtttcaac cgtcgcaagg tggtaatcgc cacgcaggtt gcgttcatga tttttgcgtt
     5581 cattctggcc atgctgacgc tgacgaagcg cgtgacggta atggaggtaa ttattctggc
     5641 ggcgctgatg ggtagcgtga acgcattcga cattccggct cgccagtcat ttttggtgga
     5701 aatggttggc cgagaagatc tgatgaacgc gatcgcgttg aactctacga tgttcaatgg
     5761 agcgcgcatt atcgggcctg cgattgcggg aattctggtg gccagcatcg gcgagggctg
     5821 gtgttttttc gcgaattcgg taagttacat cgccgtgatt gccgggttac taatgatgcg
     5881 tatcccgcct aagccggtgg taacgcatca tgcgtctccc tttgagcata ttgcggaagg
     5941 atttcggttt gtgcgcgata ccccgccgat tcgcgcgctg ctcctgctaa tcggcttggt
     6001 gagcctggtc gccatgccgt atacggtatt gatgccgatc tttgcggcga gagttctgca
     6061 cgggaatgcc aggacgctgg gaatcttgat gggggccacc ggcgtgggcg cgatgggagg
     6121 cgcgctgatg attgcgtcgc gcaggggagt tcgtggattt ggca
//
LOCUS       DATNDT010000151         6096 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3654480, whole genome shotgun sequence.
ACCESSION   DATNDT010000151 DATNDT010000000
VERSION     DATNDT010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6096
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3654480"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..409)
                     /locus_tag="VN788_04880"
     CDS             complement(<1..409)
                     /locus_tag="VN788_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19898.1"
                     /translation="MSHSAEELLEFDRLREIVGRETTCAPGRRAIQALAPHQDVEALE
                     GEFRLIQETIAYLRAGSELGFGALADPEAWFARIAVPGAVLSPAELLEVVSLAEVAGG
                     AKQTFKNEGAKYPLLAARAASLSDFRPLSQAIRR"
     gene            complement(460..1038)
                     /locus_tag="VN788_04885"
     CDS             complement(460..1038)
                     /locus_tag="VN788_04885"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HXU19899.1"
                     /translation="MTIFGSSRPVAGDAHYVEALALGAALAAKGLAVCSGGYGGVMEA
                     VSRGAKESGGRTLAVTAEFFKARANRWVEEEIRVRTWQERLFELVERGHGYVACPGGT
                     GTLVELAVVWEMLNKGAMKRKPLVVLGDFWQPIIERVREVELGHASRWGERDEPLVHR
                     ANSPSEAADYLAAHFQTHAPHASASEHESSVR"
     gene            complement(1053..2573)
                     /gene="dacB"
                     /locus_tag="VN788_04890"
     CDS             complement(1053..2573)
                     /gene="dacB"
                     /locus_tag="VN788_04890"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HXU19900.1"
                     /translation="MQTSAAGAVAAFRARVEAVLSKSDAARAYWGIFIVDADTGETLY
                     QLNSDHYFAPASNAKVFTTALALAMLGPNYRIRTTLESKSELRSDGHLGGDLVLVGRG
                     DPDISNRKFPYSGRSEREGPAEEPLAEMADRAVSKGLREVDGDVVGDDSYFPYDPYPA
                     GWTVGDIFFKFGAPVSAIDFNDNIVSVEVQPGTRMGEPALVLTEPRAAVDTMSYEIST
                     GPAGSESEFAVVRQPGAKFILLRGTIPLNASPVKLDLSMTEPAETSALALKQLLEARG
                     VKITGRARVEHAPPPESTPSGEPVIPHASAPSSPEPASATVLVEHVSQPLSEIVRVTN
                     KESQNLHAELLLRAVGRERTGVGSTAAGLQVERAFLKAAGIADGDVLLSDGSGLSPGD
                     LMTPRAMVTLLRYAAMQPWSRDFISSLPVAGVDGTLQYRMNKTAATGRIEAKTGEIEH
                     TRALSGYATTMHGERVVFAIFYNNNPRSGAGTSASVDAIADVMVETLGAPPSKKKR"
     gene            complement(2723..2935)
                     /locus_tag="VN788_04895"
     CDS             complement(2723..2935)
                     /locus_tag="VN788_04895"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HXU19901.1"
                     /translation="MKGKASITLSSDILAKVDRAAGSKQSRSAVIEQALRDLEGINAA
                     ADRLNSEATEILDYRAVNSRGWLTSK"
     gene            complement(2976..3722)
                     /locus_tag="VN788_04900"
     CDS             complement(2976..3722)
                     /locus_tag="VN788_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19902.1"
                     /translation="MRAIYILCIRQLKRYVRSPARIIGSLGQPILFLVALGFGFGSMF
                     ARAGGGSYMNFLSPGIVAMAILFNAVFSGIEIIWDRQFGFLKETLVAPVSRLEIVFGR
                     TLGGAIVAMAQGAVVLGICMIAGFRIHSALLVPLGIVFMFLIALFFTSMGTVFGSVLK
                     DMQGFPLIMNFLVMPLFFFSSALFPVESLPKVMQAALKLNPLTYGVDGLRGSLGHAFA
                     FSLVTDFVVLSALTAILLAIGSYCFSKIQL"
     gene            complement(3719..4525)
                     /locus_tag="VN788_04905"
     CDS             complement(3719..4525)
                     /locus_tag="VN788_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011035033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU19903.1"
                     /translation="MGEMIVVNDLVKNYGDFCAVNHIAFTVPEGEIFGFLGPNGAGKT
                     TTIKMLTTVLDPTSGSIRVNGHDPKHEQKQVRRSFGIVFQDPSLDDELTAFENMDLHG
                     ILYGVPRTERLKRIDMLVEFVGLSDRKSNFVKEYSGGMKRRLEVARALLHKPRILFLD
                     EPTIGLDPQTRNHIWSYIQQLSKEENVTVFFTTHYMEEADRVAANVAVIDHGKIVSQG
                     TPSELKTDTGSATLEDAFIALTGHAIREEEANAIDRMRTMGRAWRGGGRR"
     gene            4717..>6096
                     /gene="mdlC"
                     /locus_tag="VN788_04910"
     CDS             4717..>6096
                     /gene="mdlC"
                     /locus_tag="VN788_04910"
                     /EC_number="4.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF005485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoylformate decarboxylase"
                     /protein_id="HXU19904.1"
                     /translation="MSTVREATYQVLRDLGMTRIFGNPGSSELPFLKDMPADFEYILA
                     LHERSAAGMALGYAMATNEAAFVNLHSIASAGNGLSAIIDALACHAPLVITTGQQDRR
                     QILMEPFLVSRAVEVVKPYVKWAYEPLRPEDVPAAIARGYHLAMQPPRGPVFISIPMD
                     DWNKTCTPPVARKVTQTVLPETSALDEVVRAIEASKKLALVTGSQIEEDRAWHDVIAL
                     AERLNADVYAEPIASRWTFPRSHRLFRGGLLPAQKPLAAQLAAYDTIVVLGAPIFLYY
                     AFVPGDAIAPGTKLFQITNSPHDAAAALAGTGIVGNISVAAQYIASRVKPVERNVSSR
                     TKAPEPKPEHPMTTAFVFRSLNELLPRDAIIVEECPSSKGDLDRYVALDEPGSFYSVR
                     TGILGFALPTAVGLQLAHPKRRVVCAVGDGSAQYSIQALWSAVQQKAPVIFLVICNGN
                     YSALKGFRDF"
BASE COUNT         1259 a         2055 c         1538 g         1244 t
ORIGIN      
        1 cccgccgtat agcctgcgat aacgggcgga aatcgctgag cgacgcggcg cgtgccgcca
       61 gcagcggata ttttgcacct tcgtttttga atgtttgctt tgcgccgccc gccacttctg
      121 cgagcgagac cacctccagc agttccgccg gagacagcac cgcgcccgga accgcaattc
      181 gcgcaaacca cgcttccgga tcagccagcg cgccaaagcc aagctccgat cccgcgcgca
      241 aatatgcgat ggtttcctga atcagacgaa actcgccttc cagtgcctcc acatcctgat
      301 gcggcgcaag tgcctgtatt gcccggcgcc cgggcgcgca cgtcgtctcg cggccgacga
      361 tttccctcag ccggtcgaat tcgagcaatt cttctgcgga gtgcgacacg atgcctcgtg
      421 gatagatcta gagattgtag cccggttgcc tgtgggaatt cagcgcacgg aggactcgtg
      481 ctcagaagcg ctcgcgtgcg gagcgtgcgt ctgaaaatgc gcggcgagat agtccgctgc
      541 ctccgagggt gagttcgccc gatgcaccag cggttcgtcg cgctcgcccc aacgcgacgc
      601 gtgacccagt tccacttcgc gcacgcgctc gatgattggt tgccagaaat ctcccagcac
      661 caccagcggc tttcgcttca tggcgccctt gttcagcatt tcccaaacca ccgcaagctc
      721 caccagcgtt cccgtcccgc cggggcacgc gacgtagccg tgcccgcgct cgaccaactc
      781 gaacagccgc tcctgccatg tccgcacgcg aatctcttcc tcgacccacc tgttcgcgcg
      841 cgctttgaaa aattccgctg tcaccgccag tgttcgcccg ccactttctt tcgctccgcg
      901 cgaaacggct tccatcactc cgccgtaacc tccgctgcag accgcgaggc ccttcgcagc
      961 tagcgccgcg ccaagcgcca gcgcctccac gtaatgcgca tcgcccgcca ctgggcgcga
     1021 acttccgaat atcgtcacga tctccatccg ttttaccttt tctttttcga gggcggcgcg
     1081 ccaagcgtct ccaccatcac gtcggcgatt gcatcgacgg aagcggacgt gcccgctccg
     1141 ctgcgaggat tgttgttgta gaaaatcgca aataccacgc gctcgccgtg catcgtcgtc
     1201 gcgtaacccg atagcgcgcg cgtatgttca atctcgcctg tcttcgcctc gatccgccca
     1261 gtcgccgccg ttttgttcat gcgatattgc agcgtcccat cgacacccgc aactggaagg
     1321 gacgaaataa aatcccggct ccacggctgc atcgcagcat atcgcagcag cgtgaccatc
     1381 gcgcgcggcg tcatcaaatc cccgggcgag agcccggagc cgtcgctcag caaaacatcg
     1441 ccgtccgcaa tccccgccgc cttcaaaaat gctcgctcca cttgcagccc cgctgccgtt
     1501 gagccaacgc cagttcgttc acgccccacc gcgcgcagca gcagctccgc gtgcagattc
     1561 tggctttcct tattcgtcac gcgaacaatt tccgatagcg gctgcgaaac atgctccact
     1621 aaaaccgtag cgcttgcagg ttccggcgac gagggcgcac tcgcgtgcgg aatcaccggc
     1681 tcgcccgacg gagtgctctc cggcggcggc gcatgctcca cccgagcgcg gcccgtgatt
     1741 ttcactccgc gcgcttccag aagttgcttc agtgcaagcg cgctggtttc ggcgggctcg
     1801 gtcatcgaaa gatcgagttt cacgggcgac gcattgagcg gaattgtccc gcgcagcaaa
     1861 ataaacttgg cccccggttg ccggaccacc gcaaattccg attcgctgcc cgccggtccc
     1921 gtcgaaattt cgtaactcat ggtatcgaca gccgcgcgcg gctcggtgag aacgagtgcc
     1981 ggctcgccca tccgagttcc cggttgcact tcaaccgaaa cgatgttgtc gttgaaatcg
     2041 atcgccgaga ccggcgcccc aaatttgaag aaaatgtcgc ccacggtcca tcccgcggga
     2101 tacggatcgt agggaaaata actgtcatcc cccacaacgt ctccgtccac ctcccgcaga
     2161 cctttcgaaa ccgcccgatc cgccatttcg gcaagtggtt cctctgccgg cccctcccgc
     2221 tcgctcctgc ccgaataggg aaacttgcga tttgaaatgt cgggatcccc acgccccacc
     2281 aaaaccaaat ctcctccaag atgcccatcg ctgcgaagtt cgctcttgga ttcaagcgtt
     2341 gtgcgaatac ggtagtttgg accgagcatc gccagcgcca gcgccgtcgt aaaaactttt
     2401 gcattcgatg ccggagcgaa gtagtgatcg gaattcaact ggtagagcgt ctcgcccgta
     2461 tccgcatcca caataaaaat tccccagtac gccctcgccg catcggattt gctaagcaca
     2521 gcctccacgc gcgcgcgaaa agcagccacc gcgcccgcag cggaagtctg cacaccgtgt
     2581 ttacgtgcgc cgaaatcgcg cgaccctcct gaggaagccg tctctccggc gaaaagcgcg
     2641 gacagtaaca aaacgacagt gatccaagcc ccgcgagcac aacggtgcct atgaattgga
     2701 aatcgagcca tttctagcgc ggtcactttg aagtgagcca tccgcgggaa ttcactgccc
     2761 gatagtcgag gatttcggta gcttcggaat tcaggcggtc cgccgcagcg ttgatgccct
     2821 ccaaatcgcg aagagcctgc tcaataaccg cggaacgaga ttgcttcgag cctgccgcac
     2881 ggtccacctt cgccaatata tccgaagaga gcgtgatcga agcctttccc ttcatactac
     2941 agtcatacta ctcgctcttc gcgcggtcac ggcccctaca attgaatctt cgagaagcag
     3001 tagctcccaa tagccagcag aattgctgtc aaggcgctca gcaccacgaa atccgtcacc
     3061 aggctgaacg caaacgcatg ccccagcgac ccgcgcagac cgtccactcc atatgtcagc
     3121 gggttcagct tgagcgccgc ctgcatgact ttcggcaaac tttccacggg gaataacgcg
     3181 ctggaaaaaa agaacagcgg catcaccaga aaattcatga tcagcggaaa accctgcatg
     3241 tccttcagca ccgaaccgaa aaccgtgccc atcgacgtaa agaacaacgc gatcaaaaac
     3301 atgaacacaa ttcccagagg caccaacagc gcgctgtgga tgcgaaaccc ggcgatcatg
     3361 cagattccca ggaccaccgc gccttgggcc atggctacga tcgcgccgcc cagcgtccgc
     3421 ccgaaaacaa tttccagccg cgaaacaggc gcaaccagcg tttccttcaa aaaaccgaat
     3481 tgccggtccc agataatctc gatacccgaa aaaaccgcgt tgaatagaat tgccatcgcg
     3541 acgattcccg gcgacaagaa attcatatag cttccgccgc ccgccctcgc gaacatcgag
     3601 ccgaatccga atcccagtgc cacaagaaac aaaatcggct gccccagcga gcctataatt
     3661 cgtgccggcg aacgcacgta cctcttgagc tgccttatac agagtatgta aattgccctc
     3721 atcgccgacc tccgccccgc caagccctcc ccatcgtccg catgcggtca atggcattgg
     3781 cttcctcttc tcgaatcgca tgtcctgtca gcgcgatgaa cgcatcttcc agcgtcgcgc
     3841 ttccggtatc ggtttttaac tccgaaggcg tgccttgcga cacgattttc ccgtggtcga
     3901 tcaccgccac attcgccgcc acgcgatcgg cctcctccat gtaatgcgtg gtgaaaaaca
     3961 ccgtcacatt ttcttccttg ctcaattgct gtatgtagct ccagatgtga ttgcgcgttt
     4021 gcgggtccag tccgatggtt ggctcgtcga gaaaaagaat ccgcggcttg tgcagcagcg
     4081 cccgcgccac ttccagccgc cgcttcatcc cgccggaata ttccttcacg aaattacttt
     4141 tgcggtcgga gagtcccacg aattccacca gcatatcgat gcgcttcagc cgctcagtgc
     4201 gcggcacgcc atacaaaatc ccgtgcaaat ccatgttttc gaaggccgtc agctcgtcgt
     4261 cgaggctggg atcctgaaaa acgattccaa atgaacgccg cacctgtttt tgctcgtgct
     4321 tcggatcgtg cccattcacg cgaatcgatc cgctggtcgg atcgagcact gtcgtcagca
     4381 tcttgatcgt ggtcgttttg cccgctccat tggggcctag aaagccgaag atttctccct
     4441 cgggcaccgt aaaggcaatg tgattcacgg cgcaaaaatc cccgtagttc ttcaccagat
     4501 cgttcacaac gatcatttcg cccattcgtc tttttttcct cgtgatccag gctggctgcc
     4561 cgcaaaaagc ggcgagacag gatacgctgt gttcgagtcc tcgatcaatc aagatatagc
     4621 cgctcggaag cagaactttt ctgctttgcc ttccccctgc ggtatattcg ctgaggtacg
     4681 ttctcgatga atttcatcgc ggagaagcgt gcaaacatga gcacagtgcg agaggcaact
     4741 taccaggtcc tgcgcgacct gggcatgacc cgcattttcg gcaatcctgg ctcgagcgaa
     4801 ctcccttttc taaaagacat gcccgcagac tttgaataca ttctcgcctt gcacgagcgc
     4861 agcgcggctg gaatggccct cggctacgcc atggcgacga atgaagccgc cttcgtaaat
     4921 ctgcactcca tcgccagcgc cggcaacggc ctttctgcca tcattgacgc gcttgcctgt
     4981 cacgcgccgc ttgtcatcac cactggccag caagaccgcc gccagatttt aatggagcct
     5041 tttcttgtca gccgcgccgt cgaagtcgtg aagccctacg tcaaatgggc ttacgagcct
     5101 cttcgccccg aagacgttcc cgccgccatc gcacgcggct atcacctcgc tatgcagccg
     5161 ccgcgcggac ccgtattcat ctccatcccc atggatgact ggaacaaaac ctgcacgcca
     5221 ccggtcgcgc gcaaggtgac gcaaacggtt ttaccggaaa cgtcggcact cgatgaagtg
     5281 gtccgcgcga tcgaagcgag caagaaactc gcgctggtga ccggctcaca aatcgaagaa
     5341 gatcgcgctt ggcacgacgt aatcgccctc gccgaacgcc tgaatgccga cgtctatgcc
     5401 gagcctatcg cctcccgctg gaccttcccg cgctcccatc gtctttttcg cggcggttta
     5461 ttgcccgcgc aaaaacctct cgctgcgcag ctcgccgcct atgacaccat cgtcgttctg
     5521 ggcgctccca tttttctgta ttacgctttc gttcctggcg atgccatcgc gcccggcacc
     5581 aagctttttc agatcaccaa ctctcctcac gacgccgcag ccgctctcgc cggcaccggt
     5641 attgtcggca acatttccgt tgccgcgcaa tacatcgcca gccgcgtaaa gccggtcgag
     5701 cgcaacgtgt cctcgcgaac caaagcgcct gagcccaagc ctgaacatcc catgaccacc
     5761 gccttcgtct tccgctcctt gaacgagctt ttgcctcgcg acgcaatcat cgtcgaggag
     5821 tgcccctctt caaagggcga cctcgatcgc tacgtcgctc tcgacgaacc cggctcgttt
     5881 tactccgtac gcaccggaat cttgggtttt gctttgccca ccgctgtcgg tttgcaattg
     5941 gctcatccga aaaggcgcgt ggtctgcgcg gtcggtgacg gctcggcgca atattcgatt
     6001 caggcgctat ggtccgccgt gcagcagaaa gcgcccgtga tttttctcgt gatctgcaac
     6061 ggaaattatt ccgcgctgaa aggttttcgc gatttc
//
LOCUS       DATNDT010000152         3904 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_16740, whole genome shotgun sequence.
ACCESSION   DATNDT010000152 DATNDT010000000
VERSION     DATNDT010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3904
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16740"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1419)
                     /locus_tag="VN788_04915"
     CDS             complement(<1..1419)
                     /locus_tag="VN788_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXU19905.1"
                     /translation="MRRRAFIKLSGAAALGLSGAKAFADQQVPRPPSKPSYTGEPAQA
                     APPAGKADITLRIAPVPVELAPTRIISTIGYNGTSPGPVLRMREGKPITVDVINETDT
                     PEFVHWHGFLIPAEVDGTQEEETPPVPPRGRRRFQFTPGPAGTRWYHSHAMAMADLHK
                     GTYTGQFGFAMVESGQDPGRYDQELFLALRDWEPFFTSQLMDDDDAAQSGPQPERPAV
                     LDTRPNGLEVSSAMYSINDKALGFGEPLRVKQGERLLMHILNASAIENKSIALPGHQF
                     RVFALDGNPVPSPQPVDLLLLGPGERADAFVDMNQPGIWILGAPQDNIRNFGLGVIVE
                     YANQHQQPQWVPPRQSIWDYTIFGRSAPAASPPPPPDQTPDQTIDMVFTKIPSGAGRF
                     NAWLVNGKQYPHENEFVLKKGTRYRIVFRNRTDDAHPLHLHRHRFEIVDINGKKTSGI
                     IKDTVIVPYYGRATVDFTADQPG"
     gene            1512..2357
                     /locus_tag="VN788_04920"
     CDS             1512..2357
                     /locus_tag="VN788_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HXU19906.1"
                     /translation="MNLARRISVATVLVVLGGGLLALQSNGYQFYGSGPYAPVTPEQS
                     REKAEFSWSRLRFTAAQRSFGGYGSRGGFGYGGYGYGGGWTWGQDYPKADRQFLLALR
                     RLTRVNARSTEQVVDLDDDKIFNYPWVYAVDVEDWTFTPAEAKRLHDYLLKGGFLMVD
                     NFHGTADWENFMNGMRLVIPDRPVEDLSNSDEIFHVLYDLSDRFQVPGEQYVQTGRTY
                     EKDGYVPKWRAIRDDRGRVMVAICHNMHLGDAWEWADSPEYPERFASLAFRVGINYVI
                     YGMTH"
     gene            2420..>3904
                     /locus_tag="VN788_04925"
     CDS             2420..>3904
                     /locus_tag="VN788_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HXU19907.1"
                     /translation="MFQFLFKYPAATFSKGQFVLLGPWPKWALLLAILAAAAGLGWLI
                     RSRLPRAAPRLQSWRAGIIWLLQAALAIVVLILLWQPAITVAQLKPQQNIIALLMDDS
                     HSMSIADGGTTRLAQGVKLLQGGVLAELQKKFQTRLYSVDSHLTRIPSLKKLQPTAPA
                     TRIGESLKQFVAETSDLPIGAVVLLTDGDDNSGGIDLDTIAALRNRRIPVHTVGLGAE
                     EMPHDVEIDDATVAPRAMADSRLTATVAFHQQGYAGQKAMLSVRDKQKLLSSRQITFS
                     GNGKNQSETLLFNAGDAGAKALQFSIDPMPGEENRLNNGVARLVNVTSDKKRILYVEG
                     EPRWEYKFIRRAEDDDKLVQVASMLRTTENKIYRQGINDPKELANGFPSNTDDLFGYQ
                     AIIIGSVEAGYFTPTQKDLIRQFVDRRGGGLLLLGGRFALADGGWGGSELADVLPVNL
                     PNRKNTFERVPVTVELTPAGADSLICRLADDPARNAERWKTLPYL"
BASE COUNT          770 a         1029 c         1273 g          832 t
ORIGIN      
        1 gccgggctga tcggcggtga agtcgacggt ggcgcggccg taatagggaa cgattaccgt
       61 gtccttgatg atcccggagg tttttttgcc gttaatatcg acgatttcga aacggtggcg
      121 atgcaggtgc agcgggtgag catcgtcggt gcgattgcgg aagacgatcc ggtagcgcgt
      181 accctttttt aggacgaatt cattctcgtg cggatattgc ttgccgttca cgagccacgc
      241 gttgaacctg cccgcgccgc tgggaatttt ggtgaacacc atgtcaatcg tttgatcggg
      301 ggtctgatcg ggcgggggcg gcggtgatgc cgcgggagcg cttcggccga aaatcgtgta
      361 atcccagatg gattggcgtg gcggcaccca ttggggctgc tgatgctggt tcgcatactc
      421 gacgatgacg ccgagcccga agttgcgaat gttgtcctgc ggtgcgccga gaatccagat
      481 tcctggctgg ttcatatcaa caaaagcgtc tgcgcgctcg ccggggccga gcaacagcaa
      541 atcgacgggt tggggcgatg ggacagggtt gccatcgagc gcgaatacgc gaaactggtg
      601 gccgggaagc gcgatgcttt tgttttcgat cgcgctggcg ttgagaatat gcatgagcaa
      661 gcgttcgccc tgcttgacgc ggagcggctc gccaaagccg agagctttgt cattgatcga
      721 gtacatcgcc gaactgactt cgaggccatt cggacgcgtg tcgagcacag cggggcgctc
      781 gggctgtggg ccgctctggg ctgcatcgtc gtcgtccatc agctgagagg tgaagaatgg
      841 ctcccaatca cgcagcgcga gaaaaagttc ctggtcgtag cggccgggat cctggcccga
      901 ctcaaccatg gcgaagccga actggccggt ataggtgcct ttgtgcagat cggccatggc
      961 catggcgtgg gagtggtacc agcgcgtgcc cgccggtccc ggcgtgaact gaaagcgccg
     1021 ccggccgcgc ggcgggacgg gcggagtttc ctcctcttgc gtaccgtcaa cttcggcggg
     1081 aatcagaaaa ccgtgccaat ggacgaattc aggcgtgtcc gtttcgttga taacgtccac
     1141 ggtgatgggc ttgccttcgc gcatgcgaag cacggggccg ggcgaggtac cgttgtatcc
     1201 gatggtgctg atgatgcgag tgggagcgag ttcgacagga accggggcga tgcgcagggt
     1261 aatatccgct ttgccggctg gcggagcggc ttgggcaggc tctccagtgt aggacggttt
     1321 tgaaggcggg cgcggcacct gctgatcggc gaaggctttt gccccgctga ggccaagcgc
     1381 ggccgcgcca cttaatttta tgaaagccct tcgccgcacg gctcccccgc agctttcctc
     1441 gtggttggag atggatacgc caatctgtta ggctctcctt gcgcatcaaa gtatctcatg
     1501 aagtgtgatt catgaacctc gcgcggcgga tttcggtggc aacggtcctt gtggtcctcg
     1561 gcgggggtct gttagccctc caatcgaacg gatatcaatt ttacgggtcc ggcccctacg
     1621 cgccagtcac ccctgagcag agccgcgaga aggcggagtt ttcctggtcg cggctgcgat
     1681 tcacggcggc acaacggagc tttggcggtt acggcagccg cggtggcttc ggctacggag
     1741 gatatggata tggcggagga tggacctggg gccaggatta ccctaaggcc gacaggcaat
     1801 tcctgctggc actgcgccgc ttgacgcgcg taaatgcgcg atccaccgag caagtggttg
     1861 atctcgacga cgataaaatt ttcaactacc cgtgggtgta cgccgtcgat gtggaagatt
     1921 ggacatttac tcccgccgaa gccaaacgtc tgcacgacta tctgctgaag ggcggctttc
     1981 tgatggtgga caatttccac ggcacagccg actgggaaaa tttcatgaat ggcatgcgat
     2041 tggttatccc ggaccgtccg gtggaggatc tcagcaactc ggatgagatt tttcacgtcc
     2101 tatatgatct gagtgaccgc tttcaggtgc cgggcgagca gtacgttcaa accggccgga
     2161 cgtatgagaa ggatggctac gtgccgaagt ggcgggccat ccgggacgat cgggggcgcg
     2221 tgatggtcgc catttgccac aacatgcatc tgggagatgc atgggaatgg gcggacagtc
     2281 ccgagtaccc ggagcgcttc gcctcgctgg cttttcgagt gggcatcaat tacgtgattt
     2341 acgggatgac gcactgagtg tctgaggcgg cgcggtccgc cgcaattcga ttaagcggtt
     2401 ttcgagaaat gtgcgacgaa tgttccaatt tctttttaag tatccggcgg caacgttttc
     2461 aaagggtcag ttcgttttgc ttggcccatg gccgaagtgg gcgctgcttt tggcgatttt
     2521 ggcggcggct gccgggctgg gatggctgat tcgttcgcga ttgccgcgcg ccgcacctcg
     2581 attgcaaagc tggcgagcgg gaattatttg gctcttgcag gcggcactcg caatagttgt
     2641 cctgattttg ttgtggcagc cggcgatcac ggttgcgcaa ctgaagccgc agcagaatat
     2701 tatcgcgctg ctgatggacg attcgcacag catgtctatt gccgatggcg gcaccacgcg
     2761 gctcgcacag ggcgtaaaac ttctgcaggg cggcgtgctg gcagagttgc agaagaaatt
     2821 tcagacgcga ttgtatagcg tcgatagcca cctcacgcgc atccccagcc tgaaaaaact
     2881 gcaacctacg gcgcccgcga cccgcatcgg cgaaagtctc aagcaatttg tggcggagac
     2941 ttcggatctg cccatcggcg cggtcgtgct actgactgac ggagacgaca attcgggtgg
     3001 catcgatttg gacaccattg ccgctctgcg caaccggcgc atcccagttc atacggtggg
     3061 gttgggagcg gaggagatgc cgcacgatgt ggagattgac gatgccaccg tggcgccgcg
     3121 cgcgatggcc gattcgagat tgaccgcgac agtggcgttt caccagcaag gctacgcggg
     3181 acaaaaagcg atgctttcgg ttcgcgataa gcagaagctg ctcagctcgc gccaaatcac
     3241 ctttagcggc aatgggaaga atcaatctga gacgcttcta ttcaacgccg gcgatgccgg
     3301 agccaaggca ctgcaatttt cgattgaccc tatgccgggc gaggaaaatc ggctaaacaa
     3361 cggcgttgcg cggttggtga atgttacgtc ggataagaag cgcatccttt acgtcgaggg
     3421 cgagccgcga tgggaataca agttcatccg gcgcgctgag gacgatgaca agctggtgca
     3481 ggtagcatcg atgttgcgca caacggagaa taagatttat cgccagggga tcaatgatcc
     3541 aaaggaactc gcgaatggtt tcccatcgaa taccgatgat ctttttggat accaggcgat
     3601 tattattggc tcggtcgaag cgggatattt cacgcccacc caaaaggatc tgatccggca
     3661 gttcgtcgat cggcgaggag gcgggctgct gctgctcggc gggcgattcg cgctcgccga
     3721 cggtggttgg ggcggatcgg aacttgcgga cgtgcttccg gttaacttgc cgaatcgaaa
     3781 aaacaccttt gagcgcgtcc ctgttactgt tgaattaacg ccggcggggg cggacagtct
     3841 gatctgccgc ctggcagacg acccggcgcg caacgctgag cgttggaaaa ctcttcccta
     3901 cctg
//
LOCUS       DATNDT010000153         9220 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_729109, whole genome shotgun sequence.
ACCESSION   DATNDT010000153 DATNDT010000000
VERSION     DATNDT010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9220)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9220)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9220
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729109"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="VN788_04930"
     CDS             <1..1081
                     /locus_tag="VN788_04930"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXU19908.1"
                     /translation="DKSKWQVDPFGAVIQNGYLYGRGAIDDKGMTIANVAVMVALKRS
                     NVRLTRDVVLLAEGDEEQGGQAGMKFALDKYWEKIAAGYSLNEGGRVTLKDGKVQYVG
                     VQAAEKVPVNVTVTATGTSGHGSVPLPDNAILRLSDAIGKIGAYQTPYQLTPVTETYF
                     EQLSTVIDPEIGKWMRALDNPVRSDLAEKKISEASPVWNSMMRDTIAPTMLNAGIRPN
                     VVPSEAQATLNVRLLPGNSINGLLLNLKGVVNDPKITFHADTSIGAAAPSSSLDSELF
                     KTIQQATQEEFPGVPVVPMMSTGATDSAYLRLRNVQAYGLLPFPLTEEDSLRMHADNE
                     RIPLDSFRKGIEFLYRVVSDFAVAK"
     gene            complement(1099..1878)
                     /locus_tag="VN788_04935"
     CDS             complement(1099..1878)
                     /locus_tag="VN788_04935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXU19909.1"
                     /translation="MRRYSTSSSGFQFDWDWSITPAIKVLVLVCAAVFLLQELSRMFF
                     GPAGWNFWLIWFGLIPRYAIHGFIWQPFTYLFLHEGIWHILVNMFVLWMFGRDVERAW
                     GQRRFYIYYFLCGVGAGVINIIVKTILDPHGLGSALTPTIGASGAIYGVLIAAAVLFP
                     HQRVLLIPFPVAISMRVYVGIMAAIEFFGTLGAGGDNVSHICHLGGMLVGYLYLRRGS
                     YLYSYRNRVSDWKRRRMLRKFEVYQKRHRDEPPPPPDNWVN"
     gene            complement(2152..4320)
                     /locus_tag="VN788_04940"
     CDS             complement(2152..4320)
                     /locus_tag="VN788_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HXU19910.1"
                     /translation="MASKPWSSIAPPGEFHPYIPSEKVIPEFTGRAIVLGIFAGLFFG
                     AITVYVGLRAGLTVSASIPIAVLSISILRAFGKATVLENNIVQTTGSAGESIAGGVIF
                     TLPALIFLGFPLEYWSVFFLALLGGWLGVLFMIPLRRQLIVKEHGALLYPEGTACADV
                     LVAGDKGGSFASRVFWGFGLGGAYAFLMETMGFWRDTPEVQPNWLPGASFRANITPEY
                     LGVGYIIGPRVAGVIFAGGVFSWLVLMPAIKFFGGLMTQPLFPGLIPIGQMSPDDLYR
                     SYIRPMGAGAVAAAGVITLIKTMPTIVSALGAGLKDMRESRAMGAVNTAMRRVDRDFS
                     MKIVVLGSLAIIAMMWALLTFRPIPGAATTLFENFIAAVFVVVFGFLFVTVSSRICGL
                     IGNSSNPISGMAIATLMATCAMFLVLGWTGSAFSVLALTIGGVVCIASSNAGGTSQDL
                     KTGYLVGATPAKQQLALMIGVMVSVFAVGATLNLMNVGLEEYKPAHIALDINQLPSGV
                     SVQNQSFAYARKNYVLLNAIGSHVVPDGNYLYDSAVHEIDIQWVHGIGSEKASAPQAR
                     LMATVINGILQRQLPWRLVLVGVFLVIAIELLGIRSLPFAVGAYLPIDTTMAIFAGGV
                     VRWLAERGAKDKSTADSEIGPGPLYASGLIAGGGIFGLLGIVIYLFGDPNFRYHFLPE
                     HFLHIGPQVLGALASNRIFAVLMFALLGASQYYFARRKME"
     gene            4664..6649
                     /locus_tag="VN788_04945"
     CDS             4664..6649
                     /locus_tag="VN788_04945"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HXU19911.1"
                     /translation="MASTQRVLSAVLLCLAIGLLTAFPSARAGGDEWLPINPADLALK
                     DNPASPGSSAMILYREEAVDDSHHFESEYFRIKVFTDAGKKWADVEIPYSKGFENVKD
                     VRARTILPNGSIVDFSGQVYDKVVVKAGGLKIYEKTFSLPNVQPGSIIEYKYQEPFDS
                     DQYFTVMRWDVQQDLYTKEGKFTFTELSNPQETGFQYFAWRTLFLPPDARPERQHDGS
                     YTLNVHDIPAFADEPYSVPKNMLRSRVEFYYTGHGKEPTDQFWKDTGKDWGKRLDTFV
                     NKKSALESDLAQTIHPNDSPETKLREIYSRVQKIRNLSFEQEKTAEEQKREKVSANNN
                     VQDVLKHNYGTWQDINALYVGLARTAGFDATLVYVAPRDRTLFLPNYQDTSELQTDVV
                     DVKLASQDVYLDPSSPQYPYGLIPWYENAMTGLRVSKDGGVFVKIPPDDDSQSEIVRH
                     SALHLNPDGSLSGTLVVDYTGQEGAVWRSSDRNDDDTGRRKDLHDEIRSWLPSNATFT
                     ITKFSDWDDNSKPIHIEGTLTIPSDNTRVLGRIILPLSMIQSQVARSFTSAERINDVY
                     FHYPYQEEDATSIELPAGYKLESFPKVTTIAQGAIQYVIAGGAQGNTVHLTRKLVVNG
                     IEFPANKYPIVRAIFDAVKAGDANDMVLAASSTAQHN"
     gene            6606..8600
                     /locus_tag="VN788_04950"
     CDS             6606..8600
                     /locus_tag="VN788_04950"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HXU19912.1"
                     /translation="MTWFSQHPLRHNTIKALLLACLAGLCGLTAAARNHAPPWLTAAA
                     QEQIPQYPADTVAVTLFEQQQITVDANGHVQMLYRAAYKILRPEAHDTYGTLVVPFDS
                     ETKIKSINAWSIPPSGDVYEVGAKRAMETGISPDALYSDDRQKILSIPAADPGSVIGY
                     EYVQEERPYIREEEWRFQQTIPVLKASFSLQLAPGWKMDSYWVNYPDSKPQISDRGNY
                     EWDLTNIPALPHGQDFMPAFAAVRGRLAIEYYQPVSADDPVTWQSLGSWYGNLTKDSR
                     QITPEIQQEVAQLTANAHTTLDKITAITNFVHSQIRYVAIEIGIGGFQPHSADVVFQN
                     RYGDCKDMTTLASAMLEQIGVKSYYVLIDADRGVVMPNVPTLHVDHVILAIQLPPDVP
                     AEKLYATVSDPKLGKLLFFDSTDPHCPLGYVPASLQDNYGMVVTPAGGELIKLPLLPP
                     ATNRLMRVAQFTLSASGDLSAQAQEVRWGAPAEDLRWTLLDAAPKDRVKVVENFLSTF
                     LTNFQLTKAALGNLDVSDKNLIETYNFVAPNYAKQAGDLLIVRPCVLGEKELTFDPEK
                     PRQYPVEFSTSTLDTDQFSITLPPGYAPEQMPPPVNISDSFLSYKSETKMVGNVLQYT
                     RSYELKTAVVAVKDLPQLKRDFQAISVDERQLAVLHHTAP"
BASE COUNT         2263 a         2684 c         2407 g         1866 t
ORIGIN      
        1 cgacaaatcg aaatggcagg tcgatccatt tggcgccgta attcagaatg gatatctcta
       61 tggccgcggc gcgattgacg acaaaggcat gactatcgcc aacgtcgccg tgatggtcgc
      121 gctgaaacgt tccaacgtgc gccttactcg cgacgttgtc ttgctcgctg aaggcgacga
      181 agaacagggc ggccaggcag gaatgaaatt tgcactggac aagtactggg agaaaatcgc
      241 tgcagggtac tctttgaacg agggcggccg cgtgactctg aaggacggca aggtgcaata
      301 cgttggcgtg caagcggcgg aaaaagtgcc ggtgaacgtc accgtgacgg cgacgggaac
      361 ttccggacac ggatcggttc ctctgccgga caacgccatc ttgcgtctct ccgacgcgat
      421 tggcaaaatc ggtgcgtacc aaacgccgta tcagctcacg cccgtgaccg aaacctattt
      481 cgagcagctt tcgacggtca ttgatcctga aatcggaaaa tggatgcgcg ctctcgataa
      541 cccggtgcgc tcggacttgg cggagaagaa aatctccgaa gcgagccccg tttggaattc
      601 catgatgcgc gatacgattg ctcccacgat gttgaacgcg ggtatccggc caaacgtcgt
      661 accttccgaa gcgcaagcga cgttgaatgt tcgcttgctc cccggaaatt caatcaacgg
      721 tctgctgctc aatctgaaag gcgtggtgaa cgatcccaaa attacatttc acgccgatac
      781 gtcaattggt gcggcggctc cgtcttcatc cctcgacagc gaattgttca agacgattca
      841 gcaggccacg caggaggaat ttcccggagt gcccgttgtg ccaatgatgt cgacgggtgc
      901 gaccgattcc gcgtatctgc ggttgcggaa cgttcaagcg tatgggctgc tgccgtttcc
      961 gcttacagag gaagactcat tgcgaatgca cgccgacaat gagcgtattc cgctcgattc
     1021 gtttcgcaag ggaatcgaat ttctctaccg cgtcgtcagc gatttcgcag tggcaaaata
     1081 gacttcagcg aacttaattc aattgaccca attgtcgggc ggcgggggag gctcgtcgcg
     1141 gtggcgcttc tgatagacct cgaatttgcg cagcattcgc ctgcgcttcc aatcggaaac
     1201 gcgattacgg taagaataga gatatgaccc tcttcgcaga taaagataac cgacgagcat
     1261 gccgccaaga tggcaaatgt ggctcacatt gtctccgcca gcgccaagag tcccaaaaaa
     1321 ctcgatcgcg gccatgattc ccacgtaaac acgcatcgaa atcgccacgg gaaacggaat
     1381 cagcagcacg cgttgatgtg gaaatagaac cgccgccgca atcagtacgc cgtaaatcgc
     1441 tcctgacgcg ccaatggtcg gagtcagcgc gcttccgagt ccgtgcggat ccagaatcgt
     1501 cttgacaata atattgatca cgcccgcgcc gacaccgcat aggaagtaat aaatatagaa
     1561 tcggcgctgg ccccatgcgc gctccacatc gcgcccaaac atccacagca cgaacatgtt
     1621 gacaaggatg tgccagatgc cttcgtgcaa gaaaagatag gtgaatggct gccagatgaa
     1681 accatgaatt gcgtatcgcg gaattaatcc aaaccatatc agccagaaat tccatccagc
     1741 cgggccaaaa aacatgcgag acaattcctg aagaagaaaa acagcggcgc agactaaaac
     1801 aagaaccttg atcgcaggcg tgattgacca atcccagtcg aactgaaagc ccgaagagct
     1861 tgtggaatag cgccgcatgg cggtcgagtc agtgtaagcg gcaaaggccg cggagtcaaa
     1921 acggggcgaa gctcagaggg gaaactgcct cgaaaggaca aaaaagcggg gtttcaaaaa
     1981 ggctttatcc tagacacgcc tgtcagtcaa attgctcaaa gagttaacac gctgaaggac
     2041 tttggatcat ctggtgctcc atttgccccc ttttcgttga cgattgccac ttcgattaca
     2101 gttttaggcg gtggcccttt cgcaccgggg gctctatcgt ggggaatttc ttcactccat
     2161 tttgcgccgc gcgaagtaat actgcgatgc gcccagtagc gcgaacatca gtaccgcaaa
     2221 aatgcgattg ctggcgagcg cgcccaggac ttgcgggccg atatgcagga aatgttccgg
     2281 caggaagtga tatctgaaat tcggatcgcc gaaaagataa atcacgattc cgagaaggcc
     2341 aaaaatccct cctccagcga tcagtccgct ggcgtaaagg ggtccaggac caatttcgct
     2401 atcggcggta ctcttatctt tcgcgccgcg ttcggccagc cagcgcacca ctcctcccgc
     2461 aaaaattgcc atagtcgtgt caatcggcag atacgcgccc accgcaaacg gcagcgagcg
     2521 tatgcccagc agttcaatcg caatgactag gaacacgccc accagcacaa gcctccacgg
     2581 aagttgccgc tgtaaaatcc cgttgatcac ggtggccatc aaacgcgctt gcggagcgga
     2641 cgccttttcg ctcccgatgc cgtggaccca ctgaatatcg atctcgtgca ccgcagaatc
     2701 gtaaagatag ttgccgtccg gaaccacgtg cgagccgatg gcattcagca gcacataatt
     2761 cttcctcgcg taagcgaaac tctgattttg cacgctaacg cccgacggga gttgattgat
     2821 gtccagcgcg atgtgcgcgg gcttgtattc ctccaggccc acgttcatca aattcagcgt
     2881 agcgcccacg gcaaacaccg aaaccattac gccgatcatc agcgcgagtt gctgtttggc
     2941 gggagtggcg cccaccagat aacctgtctt caagtcctgc gaagtcccgc ccgcatttga
     3001 cgaagcgatg cacaccacgc caccaatcgt cagcgccagc accgaaaacg cgctccccgt
     3061 ccagcccagc accagaaaca tcgcgcacgt cgccatcagc gtcgcgatcg ccatgccgct
     3121 aatgggattc gacgagttgc cgatcagccc gcaaatccgc gacgacaccg tgacgaacaa
     3181 aaatccgaac accaccacaa acaccgcagc gatgaaattc tcgaacagag tcgtggccgc
     3241 tccgggaatg ggacgaaagg tcagtagcgc ccacatcatc gctataatcg ccagcgatcc
     3301 cagcaccaca attttcatcg agaagtcgcg atccacacgc cgcatggctg tattcacagc
     3361 gcccatcgcg cgactctcgc gcatatcttt caatcccgcg ccgagcgccg acacgattgt
     3421 cggcatcgtt ttgatcagcg tgatgacgcc cgccgcggcc acggcacccg cgcccatcgg
     3481 acgaatatac gaacggtaca aatcatccgg agacatctgc ccgatgggaa tcaggccggg
     3541 gaagaggggt tgcgtcatca acccgccgaa aaatttaatc gccggcatca gcaccagcca
     3601 cgaaaaaacg ccgcctgcaa aaatcacgcc tgcaacacgc ggtccgatga tgtatccaac
     3661 gcccagatat tcgggcgtaa tgttcgcgcg gaacgacgcg cccggcagcc agttcggctg
     3721 gacttccggc gtgtcgcgcc agaatcccat cgtctccatc aaaaacgcat acgcgccgcc
     3781 cagtccaaag ccccaaaaaa cgcggctcgc gaacgacccg cctttgtctc ccgcgacgag
     3841 cacgtccgcg cacgccgttc cttccggata cagcagcgcg ccatgttcct tgacgatcaa
     3901 ttgccgccgc agcggaatca tgaacagcac gcccagccat ccgcccagca gggccagaaa
     3961 aaacacgctc cagtattcca gcgggaagcc caaaaaaatc agcgcgggca gcgtgaaaat
     4021 cacgccgccg gcaatggatt cacccgcaga gcccgtggtc tgcacgatat tgttttcgag
     4081 gaccgttgct ttgccgaaag cgcgaagaat gctgatcgag agcaccgcga tggggatcga
     4141 cgcggataca gtcaggccgg cgcgcaggcc gacgtaaact gtgatggccc cgaaaaacaa
     4201 tcccgcgaaa atgcccagga caatggcgcg tccggtgaat tcaggaatta ctttctcgga
     4261 gggaatataa ggatggaact cacccggtgg tgctatcgaa ctccacggtt tggacgccac
     4321 gcattgcctc cgagcctcga cagcagataa cagccataag cgtacttagt atcgttgccg
     4381 ccgcaccgaa acgcaccgca aggtagcatt agatagcata ggaaacaagc aactcgcatg
     4441 aaacggctgc agaatagcca atttggggct agcctagtag tcctattgct ctctccgaaa
     4501 ctaaactcca aagtacaccc acaaagcggt tgacatcttg tcgtcgcacg gatagcgtag
     4561 ccgtcaagtt attcaaaagt acggctcatt ggggtgtgcc ggcttcgaca cgaagcatca
     4621 agtcacgttc aaagctgctc caattttacg catggagtgt gccatggctt caacacagcg
     4681 cgtattgtcc gcagttctcc tttgtttggc tattggtctg cttacggctt tcccgtcggc
     4741 gcgagccggc ggtgatgagt ggctgcccat caatccggcc gaccttgcgc tgaaggacaa
     4801 tcctgccagc cccggatcga gcgccatgat tctctaccgc gaggaggccg tcgacgattc
     4861 gcatcacttc gaatccgaat actttcgcat caaggtcttc accgacgccg ggaaaaaatg
     4921 ggctgacgtc gagattccct attccaaggg attcgagaat gtaaaggacg tccgcgcgcg
     4981 tactattctc cccaatggtt caatcgtcga tttctccggc caagtctacg acaaagtcgt
     5041 cgtcaaggct ggtggactca aaatctatga gaaaacgttc agtctgccta acgtgcaacc
     5101 cggctccatc attgagtata agtaccaaga gcctttcgac tccgaccagt acttcaccgt
     5161 gatgcgatgg gatgtccagc aagacctcta taccaaagaa ggcaaattca cttttacgga
     5221 gctctcgaat ccgcaagaga ccggctttca atatttcgcg tggagaactc tattcctgcc
     5281 gcccgacgcg cggcccgaac ggcaacacga tggcagctac accctcaacg tccacgacat
     5341 ccccgctttc gcggatgagc cctactcggt gccgaagaac atgctccgaa gcagagtgga
     5401 attctattac acgggacacg gcaaggagcc caccgaccaa ttctggaagg acacgggaaa
     5461 ggactggggc aagaggctcg atactttcgt caacaagaag agtgccctgg aaagcgacct
     5521 ggcgcaaacc attcacccaa acgactcgcc ggagaccaag ctgcgcgaaa tttactctcg
     5581 cgtgcagaag atccgcaatc tttcgttcga gcaggagaag acggccgagg aacaaaaacg
     5641 tgagaaggtc tccgccaata acaatgtgca ggacgtcctg aagcacaact acggaacttg
     5701 gcaggatatt aatgccttgt acgttgggct cgcgcggacc gcgggatttg atgccactct
     5761 cgtctacgtt gccccgcgcg atcgcacgct gtttttgccg aattatcagg acacaagcga
     5821 gctccaaacg gacgtcgtgg acgtgaagct cgcctcccag gacgtctatc tggatccaag
     5881 ctcacctcaa tatccctatg gactgattcc gtggtacgaa aatgcgatga ccggcttgcg
     5941 agtcagcaaa gatggcggcg tgtttgtcaa aatcccgccc gatgatgatt cgcagtcgga
     6001 aattgtccgc cattcggctc tccatctgaa tcccgatggc tctctttccg gaacacttgt
     6061 tgtcgattac acggggcagg agggcgcggt gtggaggtcg agcgatcgga atgatgacga
     6121 cacaggccgc cgcaaggatt tgcacgacga gataaggagt tggttgccgt cgaatgccac
     6181 atttaccatc accaaattct ccgattggga cgacaactcg aagcccattc acattgaagg
     6241 aaccctaacc atcccgagcg acaacacacg cgtgctggga cgaattatcc tgccgttgtc
     6301 tatgattcag tctcaggtag ctcgctcctt tacctcagcg gagcgtataa acgatgtcta
     6361 ttttcattat ccctatcaag aagaagatgc cacctcaatc gagcttccgg ccgggtacaa
     6421 gctcgagagc tttccaaaag taaccaccat cgcgcagggt gcaattcaat atgtgattgc
     6481 cggcggcgcg cagggcaata cagtacattt aactcgcaag cttgtggtga acggaatcga
     6541 gtttccagca aacaaatacc cgattgtgcg ggccattttc gacgcagtga aggcgggtga
     6601 tgcaaatgac atggttctcg cagcatcctc tacggcacaa cacaattaag gcacttttac
     6661 tcgcgtgtct cgccggcctc tgcggtctta cggcggcagc tagaaatcat gctcccccgt
     6721 ggctaaccgc ggcagctcaa gaacagattc cgcaatatcc tgcggatact gtagccgtga
     6781 ctctgttcga gcagcaacag ataaccgtcg atgccaacgg ccatgtccag atgctgtatc
     6841 gcgccgccta caaaatactt cgcccggaag cccacgatac ctacggcacg ctggttgtcc
     6901 cttttgacag cgagacaaag attaaatcga tcaatgcatg gtcgattccc cccagcggcg
     6961 acgtttatga ggtcggagca aagagggcga tggaaacagg catttcgccg gatgctctct
     7021 atagcgacga ccgccagaag attttgtcca ttcccgcagc agatccgggc agcgtcattg
     7081 gttatgaata tgtccaggag gaacgtccct acattcgtga agaagagtgg cgatttcagc
     7141 aaacgatacc tgtattgaaa gcttctttct ctctgcagct agcgcctgga tggaaaatgg
     7201 attcgtactg ggtgaattat cccgactcca agccgcaaat ctccgatcgc ggaaattacg
     7261 aatgggatct cacgaatatt ccggcgcttc ctcatggcca ggatttcatg ccggcgtttg
     7321 ccgcagtcag aggacgcttg gcgattgagt attatcagcc cgttagcgcg gatgatccgg
     7381 tcacgtggca atcacttggc tcgtggtacg gcaacctcac caaggacagc cggcagataa
     7441 cgcccgaaat ccagcaggaa gtggcgcaat tgacagccaa tgcgcatacg acgctagaca
     7501 aaatcacagc cattacgaat ttcgttcaca gccagattcg ctacgtcgcc atcgaaattg
     7561 ggatcggtgg gttccagccg cattccgccg atgtggtttt ccaaaaccgc tacggcgatt
     7621 gcaaggacat gaccacgctc gcgagcgcca tgctcgaaca aattggcgtc aaatcgtatt
     7681 acgtcctgat cgacgcggat cgcggcgtgg tcatgccgaa cgttcccacc ttgcacgtgg
     7741 atcatgtgat cctcgcgatt caattgccgc ccgatgttcc ggcagaaaaa ctttacgcca
     7801 cggtgagtga tccaaagctc ggcaaattgc tgttcttcga ttccaccgac ccgcactgtc
     7861 cgttgggcta tgtccccgct tcgttgcagg acaactacgg catggtcgtc acgccagcgg
     7921 ggggcgaatt gatcaaactg ccgctgcttc cgccagccac gaatcgcctc atgcgcgtcg
     7981 cgcagttcac actcagcgcg tcaggcgatc tttcagcgca ggctcaggaa gtccgctggg
     8041 gagcgcccgc cgaggatctt cgttggacgc ttctcgatgc cgcgccgaag gaccgcgtca
     8101 aggtggtcga gaatttcctc agcacgttcc tcacgaattt tcaattgacg aaagccgcgc
     8161 tcggcaatct ggatgtctcc gacaagaatt tgatcgaaac ttataacttt gtggcgccca
     8221 attacgccaa gcaggcggga gacttgctca tcgtgcggcc ttgcgtgctc ggtgaaaaag
     8281 aactgacttt cgatccggag aagccccgcc aatatcccgt cgagttttcc acctcaacac
     8341 tcgacacgga tcaattcagc atcactttgc ctcccggcta tgctcccgag cagatgcctc
     8401 ctcctgtgaa catcagcgat tcttttcttt cttataaaag tgaaacgaaa atggtcggca
     8461 acgtgttgca gtacacgcga agctacgaac tcaaaaccgc cgtcgtggcc gtgaaggact
     8521 tgccccagct caagcgtgat tttcaggcca tttccgtcga cgagcgccag ctcgccgtcc
     8581 tccaccacac ggccccgtaa gccgcatcga tccggacggc caggcggttg gaattgcatg
     8641 tgctcgggca tcaccacata gcccacgaga gaaaactggt ggcgattcct tgcctctccc
     8701 agcgcctgca caaaaagact tcgtgcacgg gccatgccca gcagttgttg tctccgatgg
     8761 caactaaacg tgatgaaatg caaatgcccg tggccataaa ttcgaagcaa tttatttcgc
     8821 atgtttcgag cctgaagaac cgaagaaaac ccgcaccctt agaaaccaaa gggtgcggca
     8881 cccacggcca aaccaagtaa gtcactgttc ggtgaactgg cgcggtggta tcatccgggc
     8941 gctgttgcta tgtcacaaaa ggaaaacgtg tcgccgaaga tgcgccaccc gcccttctgc
     9001 tttccgcagc cctctttgag tccttcggtg ggcgtcccag cctaaagaag cacttccgga
     9061 gccacacact tgctaaagca actcctcatt cgagtgctca ttggcgggag cgtggtctcc
     9121 gtgttttccg tgctcggaga tttgtttgaa cccaagagtt ttgccggcct tttcggatcg
     9181 gcgccgtcca ttgcgctggc cactttggga cttacgttgt
//
LOCUS       DATNDT010000154         2398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3454648, whole genome shotgun sequence.
ACCESSION   DATNDT010000154 DATNDT010000000
VERSION     DATNDT010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3454648"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VN788_04955"
     CDS             complement(<1..528)
                     /locus_tag="VN788_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HXU19913.1"
                     /translation="MSTLTHPETKSSATPATQNSAEQNALMFEYSSAADPVSSGSVPP
                     IPYAEFAADLYATGPSRVIPLDLSKALKSAAPASTPSLAASFLRIEADDTLETSANST
                     SELFYVIRGSGMSKVGDQEIHWSEGDFFTLPGSTARHRALETSALYWVNDQPLLDYLG
                     VQRSHPRFAAAHYTRG"
     gene            771..1040
                     /locus_tag="VN788_04960"
     CDS             771..1040
                     /locus_tag="VN788_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19914.1"
                     /translation="MTENNDGRSWWRRIFGRSLLKMVVGDFFSDKNNAASIIAILLVA
                     TLCYVVAFRQKYEYVNQLLNIVFVVIGYYFGSKRERDADDDDSGS"
     gene            complement(2021..>2398)
                     /locus_tag="VN788_04965"
     CDS             complement(2021..>2398)
                     /locus_tag="VN788_04965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HXU19915.1"
                     /translation="SAFYGPFREAADSAPQFGDRRSYQMDGANRREAMREIDLDLSEG
                     ADMILMKPAMPYLDIIREARDRFDVPLGAYQVSGEYSMLRAAIDRGWLDRDRAILESL
                     VSIRRAGADFIVTYFAQAAAKLL"
BASE COUNT          547 a          594 c          728 g          529 t
ORIGIN      
        1 gccgcgggtg taatgggccg cggcgaagcg cggatggctg cgctgcacgc ctagatagtc
       61 gagcaatggc tgatcgttga cccagtaaag cgcggaggtt tccagggcac gatgacgtgc
      121 ggtgctgccg ggcagcgtga agaaatctcc ctcggaccag tggatctctt ggtcgccgac
      181 tttggacatg ccgctgccgc ggatgacata aaacagctcc gaagtcgagt tggcggaggt
      241 ctccaacgtg tcatcggcct cgatgcgcaa aaagctggcg gcgagcgatg gcgtgcttgc
      301 cggggcggca gacttgagcg ccttcgaaag atcgagtggg atgacgcggc tgggcccggt
      361 cgcgtacagg tctgcggcga actccgcgta gggaatcggc ggcacgcttc ccgacgaaac
      421 gggatcggcg gcagagctgt actcgaacat cagagcgttt tgctcggcgc tgttttgtgt
      481 ggctggggtt gcggatgact tggtttcggg atgggtgagc gtactcatgg atcaacctcc
      541 gtcagcggct ggcgccgctg atttcattat aggaactgac gcaggttttc gtctcgcgtg
      601 cgtttgtggg tgtccatacg cgccgcgttt tggcagcgac gtgggggaaa agcaaacacc
      661 agcaatctat cgtgaaactt tggtggcgct gcctaaatca gggcgatgac aacatgcggg
      721 ctattctaaa ggcattcgag tctaaaggcg ctcgaaaact aggggattag atgacggaaa
      781 acaatgacgg aagaagctgg tggagaagaa tattcggtcg aagccttctc aaaatggttg
      841 ttggggattt tttctccgat aaaaataatg cagcgtcaat cattgcgata ctcctggtcg
      901 ccaccttgtg ttatgtcgta gcatttcgcc agaagtatga atacgttaac cagctgctta
      961 acatagtatt cgtcgtgatt ggttattact tcggatcgaa gcgcgaacgc gatgccgacg
     1021 atgacgactc gggaagctaa taggcggaaa tcatgtttgt cttacggaac ggtcttcgtc
     1081 aggcgtgtga gcgattgcgc gttttcttga cacttgagtc attcatacct aaggtgtgac
     1141 taaattgagg taaccgtgtg aattcgagca gggtatcgag gccaaagttt tgcaatctct
     1201 tatgtctggc gttaactatt gcggaagtat tagcatttcc aattccctct ttcgcccgtt
     1261 ccaagccaat tccacttcaa aaatctttcg atgctccagt tgagcgtgtt ttcgcggcag
     1321 aagcagaagc ggctggttcg accatcgtaa atacagtcag ggccgcctgc ttggtgaact
     1381 tccagaccaa agcttacttc ggaaacgggt tctatcgtgt gattagctgg acggctgttt
     1441 gcaaagacgc cggaagcggg aaaacaaccg ttacgctttc aggacgggtg aactcaaact
     1501 cgttcggaaa tgggggagcc gaaaggaagg atgccactac tttctggtcc aacatggatg
     1561 cagccctagc gaacggcaga tcgtcgagcg tatccgcatc catttccgcg acttcgcagc
     1621 ccatcccgaa agctggacag tcagtcccgg taccttccga ggcagttgtt cagatatctt
     1681 ctgagccgag cgacgctgac ttttcaattg atggagatta ccaagggaac acccccagcc
     1741 aaatcaaact caaacctggt tctcattcca taatgatcag caaggccgga ttcaacccct
     1801 gggagagatc aatcaatgta aaagacggtg aggttcgcaa tatctccgcg cggttggaga
     1861 aaccaaataa atgactaaaa gtcgaagaat ggcgccgttg agagatccaa ggtcccatat
     1921 tcgagaccct ttggcaatct tagggcaggc gctggcgcgt ccgacgaatt gttgaaagtc
     1981 ggatccctcc tctctgcagc gtttcggtcg gggtctctgg ctacaacagc ttggcggcgg
     2041 cctgggcgaa gtaggtgacg atgaagtcgg ccccggcgcg gcgaatggag actaaggatt
     2101 ccagaatggc gcggtcgcgg tcgagccagc cacggtcgat ggcggcgcgc agcatggagt
     2161 attcgccgga aacctggtag gcaccgagag gaacgtcgaa gcgatcgcgg gcctcgcgga
     2221 tgatgtccag atagggcatg gccggcttca tcaggatcat gtcagcgccc tcggagagat
     2281 cgaggtcgat ttcgcgcatg gcttcgcggc gattcgcgcc atccatctga tagctgcggc
     2341 ggtcgccgaa ttgcggcgcg gaatcggcag cctcgcgaaa cgggccataa aacgcgga
//
LOCUS       DATNDT010000155         3921 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4094010, whole genome shotgun sequence.
ACCESSION   DATNDT010000155 DATNDT010000000
VERSION     DATNDT010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4094010"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(489..1706)
                     /gene="argJ"
                     /locus_tag="VN788_04970"
     CDS             complement(489..1706)
                     /gene="argJ"
                     /locus_tag="VN788_04970"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXU19916.1"
                     /translation="MNAPLSASDIPSGFTFSATHCGLKRSRLDLGILVSDTPASAAAV
                     FTTNQVVAAPVVLSRKHLRKSRKRMRGLIVNSGNANCCTKQDGYPASVATASKLADEL
                     GGIDPSQILVCSTGVIGAPLRVEKILAAIPHLALTRSADPAAFEEFARSIMTTDTRPK
                     WAIARRRIGGKEVRFLGCAKGSGMIQPNMATMLAFVATDAAISPPLLSRGLRDVVGST
                     FNSITVDGDTSTNDTLAVLANGGSGAPRISSARGSDYQRFVAALEGVCASLALSIVDD
                     GEGAQRVIEIQVKGAPSDSAADQVARAIANSPLVKTAFAGADPNWGRILAAAGRSGVR
                     FNLGRTDISIAGVAVCRYGREHSFDERAVHRKMLAKHVPVRVDLRSGLGAARIWTCDF
                     TIEYVHINSSYRT"
     gene            complement(1720..3129)
                     /gene="argH"
                     /locus_tag="VN788_04975"
     CDS             complement(1720..3129)
                     /gene="argH"
                     /locus_tag="VN788_04975"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HXU19917.1"
                     /translation="MTVSEGQKMWGGRFDRGPDASFYEFERSWKFDRRLLPYELALDR
                     AWARAIAAAGILTREESQSLVGALDEIERRAQSDSAWLDTSKAEDVHHFAETALIEKL
                     GPLGAKLHTGRSRNEQVTTEFRMYVKDATREIRAALASLLRAIATQARSNLGIPMPGT
                     THMQHAQPLLLSHWLLAHGEALQRDSERLRAAAAAADSCPLGSGALAGCAFPLDRKAV
                     AHDLGFSRITANSLDAVSDRDFALEFLFALATLAMHLSRLSEDMILFASPEFGFIDLP
                     DEYSTGSSLMPQKKNPDSWELIRGKTGRIYGSLFALLTTCKGLPSSYQRDLQEDKEPL
                     FAAHDQIVPMLRIAAGALAGTHFREARLREAAQDPALVATEVADYLVAQGIPFREAHE
                     IVGKLLRAADQDGKSIREMPLDRLRQFSPVFGAELSTVLTLESALARRAVAGGTALGA
                     VRAALDDFTSRLAKLEENT"
     gene            complement(3126..>3921)
                     /locus_tag="VN788_04980"
     CDS             complement(3126..>3921)
                     /locus_tag="VN788_04980"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HXU19918.1"
                     /translation="LAPELTIIAPWREWKINSREEAIAYAREHNIPVEQTTKNIYSRD
                     RNIWHLSHEGGVLEDPANAPEESMWQWIVSPAKAPDQPMTVEIEFEAGTPVSVNGRKF
                     GPLALLNQLNEIAAANGVGRMDIVENRLVGMKSRGAYETPGGTLLVVAHRELESLCLD
                     RETMHYQQLLSLRYAEMVYYGMWFTPLRESLDAFFTRSQQRVTGSVGLSLFKGNVNPT
                     SRRSPHSLYRAGLASFSMTGYNPKDAEGFINLFALPITVPVEAGVR"
BASE COUNT          830 a         1116 c         1209 g          766 t
ORIGIN      
        1 cccgcggcca gttgtaaatc gacgcgcggc tcagggctga agcgagcatc ccagcaactc
       61 aggagcatct tgctgcaact gcccccccca gtcaactcat tggcccatga aagggtgtga
      121 agtgccaggc ggagcggatg ggagcatcgg cgactcagtc gcgcggcaag ctccgcccgc
      181 ttggcgacga atccagccgc taccgctaca ttgtaatgtc catgcgcatc tgagcgattg
      241 tcgttttgaa cagctcagcg tccatactgt tgctgggagt ttgccaagcg aggccgaaga
      301 gggcgcagta cgaggataca agacaaattg tggattaaag gcgtggaatg atgcaagtgg
      361 ttgttggtgt aaagaaaaat cgtccacggt gggtgtggtg aatgatgcga tttcgacgtg
      421 cttgagctgg gtgccagcta agaaaggggc cgcgaacgag gtccaaggtt tttttcacac
      481 aaattttctt acgtgcggta gctggaattt atgtggacgt attcgatggt gaagtcgcaa
      541 gtccagatac gggcggcgcc gagaccggaa cgcaagtcga cgcgaaccgg gacgtgcttt
      601 gccagcattt tccggtgcac ggcgcgttcg tcgaaggaat gctcgcggcc gtaacggcaa
      661 acggctaccc ccgcgatcga aatatccgtg cgccctagat tgaaccgcac gccggagcgg
      721 ccagcagcgg caaggatccg gccccagttg gggtcagctc cggcgaaagc ggtcttcact
      781 agcggggaat tggcgatcgc gcgcgcgacc tgatccgcgg cgctgtcgga cggcgcgcct
      841 ttgacttgga tttcaatgac gcgctgagcg ccttcgccat cgtccacaat agaaagtgcg
      901 agtgacgcgc agactccttc gagagcggca acgaatcttt gataatccga gccacgcgcg
      961 ctggaaattc gcggcgcgcc ggatccaccg ttcgcgagaa ctgccaacgt atcgtttgtg
     1021 gaggtgtcgc catcgactgt gattgaatta aatgtcgagc cgaccacgtc ccggagacca
     1081 cgagaaagca gcggcggaga tattgcagcg tccgtggcga cgaaggcgag catcgtcgcc
     1141 atattgggtt ggatcatgcc ggaacctttt gcacagccca ggaagcgcac ctccttccct
     1201 ccgatacggc ggcgggcgat ggcccacttc ggccgcgtgt cggtggtcat aattgaacgc
     1261 gcaaattctt cgaaggccgc tgggtcggcg ctgcgcgtta aggccaagtg cggaatagcg
     1321 gccagaattt tttcgacgcg caacggcgcg ccgataacgc ccgtggagca gaccagaatc
     1381 tgcgaaggat cgatgccgcc cagctcgtcg gcgagctttg aggccgtggc gacagatgcg
     1441 ggatagccat cttgctttgt gcaacagttc gcgttgccgg agttaacgat caggccgcgc
     1501 atgcgcttcc gcgatttgcg caggtgcttg cgcgacaaaa cgaccggcgc ggcaacaact
     1561 tgattcgtcg tgaagacggc agcggcgctg gccggcgtgt cgctgacgag gatgcccaga
     1621 tcgagcctcg agcgtttgag gccgcaatgg gtggcggaga aagtgaatcc cgaaggaata
     1681 tcagaggcgg agagcggcgc gttcatgcga aacgcgccct cacgtatttt cctcaagctt
     1741 cgcgagccgg gaagtaaaat catcgagcgc ggcacgaacg gctcccagag ccgtgccgcc
     1801 ggcgactgct cgcctggcga gcgccgattc gagggtaagc actgtgctta gctccgcacc
     1861 aaatacgggc gagaactgcc gcagccggtc aagcggcatt tcgcgaatcg atttgccgtc
     1921 ctgatccgcc gcgcgaagca acttgcccac gatttcgtga gcttcgcgaa atggaattcc
     1981 ctgagccacc agataatccg ctacctcggt ggccacgagc gccggatctt gcgctgcctc
     2041 gcggagccgt gcctcgcgga agtgcgttcc cgccagcgca cccgccgcaa ttcgcaacat
     2101 ggggactatt tgatcgtggg ccgcgaaaag cggttccttg tcctcttgca agtcgcgctg
     2161 ataacttgag ggcaggccct tgcaggtggt aagaagagcg aaaagcgaac cgtagatgcg
     2221 gcccgtcttg ccgcgaatca gctcccacga atctggattt ttcttttgcg gcatcaagct
     2281 gctgccagtg gagtattcgt ctggcagatc aatgaatccg aattcaggcg aggcgaagag
     2341 aatcatgtcc tctgaaagac gtgacagatg catagccaaa gttgcgagcg caaacagaaa
     2401 ttcgagcgca aaatcacggt cgctgaccgc atcgaggctg ttggcagtga tgcgcgaaaa
     2461 accaagatcg tgcgcaaccg ccttgcggtc cagcggaaat gcgcagcccg ccaatgcgcc
     2521 cgagcctagc ggacacgaat cggcggcggc cgcagcagct ctaagccttt cggagtcccg
     2581 ctgaagagcc tctccatggg ccagtaacca gtgtgaaagc agaagcggct gtgcatgctg
     2641 catgtgcgtg gtacccggca tcggaatacc gagattgctt ctggcctgcg tggcaatcgc
     2701 acgcaatagc gaagcgagtg ccgcccggat ttcgcgtgtc gcgtccttca catacatccg
     2761 gaattcagta gtcacctgct cgttgcggct gcggccggta tggagcttcg cgcccaaagg
     2821 gccaagtttc tcgatcagcg cagtttcggc aaaatggtga acgtcttcgg cctttgaggt
     2881 atcgagccac gccgaatccg actgtgcacg cctctcgatt tcatcgagag cgcctacaag
     2941 actctggctc tcttcgcgcg tgaggattcc agcagcggca atagcacgag cccaggcccg
     3001 gtcgagagcc agttcgtacg ggagaagcct gcgatcgaac ttccacgagc gctcgaactc
     3061 gtaaaaagaa gcgtccgggc cgcggtcaaa gcggccaccc cacattttct gtccttcgga
     3121 aacggtcatc gcaccccggc ttcaaccgga accgtgatag gcagagcgaa aagattaatg
     3181 aagccttcgg cgtccttcgg gttgtagccg gtcatgctga agctggctag cccggcgcgg
     3241 tacaaggaat gcggtgaacg gcgagaagtc gggttgacgt tgcccttaaa gagcgacagc
     3301 ccgacggaac ctgtgacgcg ctgctgtgat cgcgtaaaga acgcatcgag ggattcgcgc
     3361 agcggtgtga accacattcc gtagtagacc atctccgcgt aacgtaaaga aagaagctgc
     3421 tgatagtgca tcgtctcgcg atcaaggcac agcgattcga gctcgcgatg cgctacaacg
     3481 agcagggtcc cgcctggcgt ctcgtacgcg ccccgggatt tcatacccac caggcggttt
     3541 tcgacaatgt ccatgcgccc cacgccgttg gctgcggcga tttcgttgag ctgattgagg
     3601 agggcgagag gaccgaattt cctgccattt acggaaacgg gagttccagc ttcgaactct
     3661 atctctaccg tcatcggctg gtcaggagct ttcgctggcg aaacgatcca ttgccacatg
     3721 gattcctctg gcgcgttcgc cggatcctcg aggacgcctc cctcgtggct caagtgccaa
     3781 atattgcggt cgcggctgta gatgttcttt gtggtttgtt cgaccggaat attgtgctcg
     3841 cgggcatacg cgatcgcctc ttctcgcgag ttgatcttcc attcacgcca cggggcaatg
     3901 atggtgagtt cgggtgccag c
//
LOCUS       DATNDT010000156         5564 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2721581, whole genome shotgun sequence.
ACCESSION   DATNDT010000156 DATNDT010000000
VERSION     DATNDT010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5564)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5564)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5564
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2721581"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..990
                     /locus_tag="VN788_04985"
     CDS             <1..990
                     /locus_tag="VN788_04985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU19919.1"
                     /translation="QLRAMRDGRRVASVFGVLRRADPPFLAAANDPDDPLPNVSVKIR
                     SRDDRFETSTDADGVYRFYDVHAGEYSLTADLPAGTGLTQKSIGSLQPFSIPDGACFE
                     YDVDALPTGSIHGSVLGPNGKPLDIASLELYRVGNYSSSRPGLWAFQGAEGAFNFDHI
                     GPGDYILVFNRANRKNPNAPFKRAFFPGVADLSEAQPIHLDDGQNLSKIDINVSDRYP
                     TRQLRVQLKWIGARPPGSVTVGVKADQGENPAADEIGNNLYEVTLLESASYKISAWED
                     LDPGRVPSSRRRKVDCPTPARVDATSVSVLGSDANVKKVTLTFAKPGCGENQQ"
     gene            1025..2386
                     /locus_tag="VN788_04990"
     CDS             1025..2386
                     /locus_tag="VN788_04990"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HXU19920.1"
                     /translation="MADIRPFRAYRYDSHRVALQDVLTQPYDKITPAMQDRYYAASPY
                     NLIPVEKGRALPDDSPEENVYTRAARKIDEWIAEKIIVQDAAPNIYVYSQEFCVPGTK
                     SRRTRIGFIALARLENYEAHVVFRHEHTLSGPRADRIELLRHTRAQTGQLFLLYDDPS
                     KQIDTWLDQVAHTSPDVELHDEFGVTHRMWPVPNPAFAERIENAMAGKSIVIADGHHR
                     YETALSYRDECRLRAGKADPQAAYEFAMATFINTHSKGLTILPTHRLVRNLPGFDFDH
                     FRAVLTPYFDWYAYPFVSADDRAGVYAEFRKDLERPNHGRRAIGAYPRAAQSGVDAFY
                     LFVLRRGVDLESLLPDLSEGQRGLDVVLLHRLILNKGLGITAEAVTAEKNLTYEREME
                     AAIASVDRGDAQLALLLNPVRVDQVMKIALAGDVLPQKSTDFYPKMLSGIAVYRIEGR
                     VGE"
     gene            complement(2793..3803)
                     /locus_tag="VN788_04995"
     CDS             complement(2793..3803)
                     /locus_tag="VN788_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007767215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU19921.1"
                     /translation="MRKVDIFTHIWPKAFHDALKKQTGQMKDMHRRSEAVPIMTNLDM
                     RLKMMDTFGEYQQILSLASPPPDALGDPVSGRELSKIGSDGMAELCRKYPDRFPSFIA
                     SIAMTDAEGAAEEARRAIKDLGACGVQIYTNVNGKPLDSGEFRHIFKAFEALGKPVWV
                     HPARTAKFPDYQTEELSQYEIWWTFGWPYESSAMQARLIFSKMLDEMPNLKVIIHHAG
                     GMTPFFEGRVGAGLDQLGSRTSNVDYKPLLKQLKHRPFDYFKMFYVDTATFGALGALE
                     CALKFYGVDHVLFASDSPFDPEGGPMYIRETIRCVDALDISKEDREKIYYRNACKLLG
                     IR"
     gene            complement(3823..4644)
                     /locus_tag="VN788_05000"
     CDS             complement(3823..4644)
                     /locus_tag="VN788_05000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19922.1"
                     /translation="MAGLLQGQIAIVTGAGRGIGKAIALRFASEGAAIAVTSRNQAQL
                     DQVLAQIEGAGGRGLAAAGDVTKAADVARVARATVEKLGGATILVNNAGIGGPYGPVG
                     VLDPEAWWAAQEVHILAPFLFMSAVLPGMIERRAGRIINISSPRCHMLTANLSAYSLG
                     KTAQNRLAELVANEVKEYGVKAFALDPGSIMTEMADETMGSRDAQRWVPKMVERLHEL
                     KGKVGPQDGMAWCTDRCLAFAAGRYDALSGRYVDRTDDPEEMLKHPQPKVPRANP"
     gene            complement(4653..>5564)
                     /locus_tag="VN788_05005"
     CDS             complement(4653..>5564)
                     /locus_tag="VN788_05005"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HXU19923.1"
                     /translation="HFFGYEASGLATRDVSYCVADKEGQLVKEEWFQVPYVSLMHDFA
                     VSKEHVIFPVFPTTADLDRMKAGGTHWVFEPEKDAYVGIMPIDGHTKDMRWFRHPACS
                     AFHFMNAYTEGKFVHLDFGIGKYAPFPFIQEASHIHPKAEDFAYGRVVRWTFDMSKPG
                     EKFEEYKLAPSGDFPRIANKDHMKDYSIGYYERFDPEVGPPMVAGPVGAGFNTLSRLE
                     IKSGKMKSWRPTRPVTIQEEVHIPSRTPGHEGYLALVADLHDTQLSDLLVFEAEHLDK
                     GPIATAHLPLRLRNQVHGNWVTAEELQ"
BASE COUNT         1167 a         1620 c         1555 g         1222 t
ORIGIN      
        1 caattgcgag ccatgcgtga tggacgccga gtggcatccg tttttggcgt tctgcgccgc
       61 gctgatccac ctttcttggc tgccgcaaat gatcccgatg acccgttgcc caacgtatct
      121 gtgaaaattc gttcgcgcga cgaccggttt gagaccagca cggatgcgga cggcgtctac
      181 cgcttttacg atgttcacgc aggcgaatac agccttacgg cagaccttcc tgccggcact
      241 gggctcactc aaaagtctat tggaagcttg cagccattct caattccaga tggcgcatgt
      301 ttcgaatacg atgtcgacgc gctccccaca ggatccattc acggcagtgt gctcgggcca
      361 aatggcaagc cgctggatat tgcctccctt gaactttatc gtgttgggaa ctatagctct
      421 tctcgccctg gtttgtgggc gttccaggga gccgaaggcg catttaattt cgatcatatc
      481 ggcccgggag actacatcct cgtattcaac cgtgcgaatc gcaagaaccc gaacgcgcca
      541 ttcaagcgcg catttttccc cggtgtcgcg gacttgagcg aagctcaacc gattcatctc
      601 gatgatgggc agaacctctc gaagatcgat attaacgtca gcgatcgtta tccaacgcgg
      661 cagcttcgcg tgcaattgaa atggatcggt gcgaggccgc ctgggagcgt tacggtgggg
      721 gttaaagccg accaaggcga gaatccggcc gccgatgaaa tcggaaataa tttgtatgaa
      781 gttactctgc tcgaatccgc gagctacaag atttccgcgt gggaggatct cgatcctgga
      841 cgcgttccat cgagcaggcg gcggaaggtc gattgcccta ccccagctcg cgtcgatgcc
      901 acgtcggtga gcgtgttagg ctccgacgcc aacgtgaaga aagtgacttt aacatttgcc
      961 aagccgggat gcggcgagaa ccagcagtaa agccacacgg aacgacgctg tttgaggact
     1021 caccatggct gacatccgac cttttcgcgc ataccgctat gattcccacc gagtagcgct
     1081 ccaagatgtc ctgacacaac cctacgacaa aatcactccc gcaatgcagg atcgctatta
     1141 tgcggcgagc ccctacaatc tgatcccggt ggaaaagggc cgcgccttgc ccgacgattc
     1201 acccgaagag aatgtttaca ctcgcgccgc gcgcaaaatc gacgaatgga ttgcagagaa
     1261 aatcattgtg caagacgcgg ctccaaacat ttacgtgtat tcgcaggagt tttgcgtccc
     1321 cgggacgaag agccgccgca cgcggatcgg ttttatcgcc ctggcgcggc tggaaaatta
     1381 cgaagctcac gtcgttttcc ggcacgagca cacgctgtcg ggtccgaggg ccgatcggat
     1441 cgaactgctg cggcacacgc gcgcccagac ggggcagctc tttcttctgt acgacgaccc
     1501 gtcaaaacaa attgatacct ggctcgatca ggttgcccac acgagcccag acgtcgagct
     1561 gcacgatgaa ttcggcgtga cgcaccgtat gtggccggtc ccaaaccctg cgttcgcgga
     1621 aagaattgag aacgcgatgg ccgggaagag catcgtgatc gcagacgggc atcatcgtta
     1681 tgaaacagcc ctcagttatc gcgacgaatg ccgcttgcgc gccggcaaag ccgacccgca
     1741 agcggcctac gaattcgcga tggcgacatt tattaatacg cattcgaagg gcctcacgat
     1801 tttgccgacg catcgactcg tgcgcaatct gccgggcttc gatttcgacc actttcgcgc
     1861 ggttcttact ccctatttcg attggtatgc ctatcccttt gtaagcgcgg atgaccgcgc
     1921 aggggtctac gccgaattcc gaaaagactt agagcgtcca aatcacggcc ggcgcgccat
     1981 tggcgcatac cctcgcgcgg ctcaatccgg cgtggacgca ttctatcttt ttgtgctgcg
     2041 gcgtggcgtg gatcttgagt cgttactgcc cgatctttcc gaagggcaac gcggcctgga
     2101 tgtagtcctg ttgcaccggt tgatccttaa taaaggattg ggaatcaccg cggaagcagt
     2161 gaccgccgag aaaaacctca catacgagcg ggagatggaa gcggccatcg cttcggtcga
     2221 tcgcggagac gcgcaactgg cgctgctttt gaatccggtc cgcgtggacc aggtgatgaa
     2281 gatcgcgctt gcaggcgacg tgctgccgca aaagtcgact gacttctatc caaagatgtt
     2341 gagcgggatc gccgtgtacc ggattgaggg gcgagttggc gaatgagaat tgccgcgtgt
     2401 gtgacgagta gaatgtagcg cggcgtttgt tgtcaatgac ttgcggcgat ttctactcgc
     2461 cgtgtgtgtc gattagaaat tgggcttctc ggttgcatta atctaccgcg cagggcccat
     2521 ttcgccagtt tagtcctcct tcctttccca cagcatgacg ctcgttagta cgggtcggca
     2581 cgccgaccag gaagtaaaat actcccaagt tacaatactg tgcgaggagt tcgatttcaa
     2641 ttgtggaaaa tacggatacc gcaaaccggc tgcgaagagc cggtgacatg ctggtgcgac
     2701 aagggcccag gacgctagca tacccttcgc aacaatcgcc agcgccctac gagatttccc
     2761 gccccacctg ggatcacagc acgaagtgag agctaccgga tgcccagaag cttgcaggca
     2821 ttcctgtagt agattttttc gcggtcttct ttcgaaatat caagggcatc aacgcaacgt
     2881 attgtttcgc gtatgtacat cggaccgcct tcagggtcga atggggaatc agacgcgaaa
     2941 agtacatgat ccacgccgta aaatttcagg gcacattcga gcgcacccaa cgcgccaaac
     3001 gtggccgtat cgacgtaaaa cattttgaaa taatcaaagg ggcgatgctt caactgcttc
     3061 agcagcggct tgtagtccac attggaggtg cgcgagccaa gctgatcgag tcccgcgccg
     3121 acgcgccctt cgaagaaagg cgtcatccct ccagcgtgat ggatgatgac tttgaggttc
     3181 ggcatctcat cgagcatttt tgagaaaatc aaacgtgcct gcatcgcact cgattcataa
     3241 ggccatccga aggtccacca gatttcgtac tgtgaaagtt cttcggtctg atagtcgggg
     3301 aatttcgccg tgcgcgcggg atgaacccag acgggtttac caagagcctc aaacgctttg
     3361 aaaatgtggc ggaattcgcc cgagtccagc ggcttgccat ttacattggt gtaaatttgg
     3421 acgccgcacg cgccgagatc cttgatcgcg cgtcgcgctt cctcagccgc gccttcggcg
     3481 tccgtcatcg caatggaggc gatgaagctt ggaaaacgat cgggatattt tcggcaaagc
     3541 tcggccatcc cgtcacttcc gatttttgag agttcgcgtc ccgaaactgg atcgcctagc
     3601 gcgtcgggcg gcggcgatgc gagcgaaaga atctgctgat actcgccaaa cgtgtccatc
     3661 attttcaagc gcatgtccag attggtcatg attgggacag cttcgctgcg gcggtgcatg
     3721 tccttcattt gccccgtttg cttcttgagt gcatcgtgaa acgccttcgg ccaaatatgg
     3781 gtgaaaatat cgactttcct catgatcgcg ttggtctccg tatcaaggat tcgctcgcgg
     3841 gactttgggt tgcgggtgtt tcaacatctc ttctggatcg tccgttcgat cgacataacg
     3901 cccggaaagc gcgtcgtatc gccccgcggc aaacgcgaga caacggtcgg tgcaccacgc
     3961 cattccgtcc tgcggtccaa ccttgccttt caactcgtgc agccgctcca ccatttttgg
     4021 cacccaacgc tgcgcgtccc gcgaccccat cgtctcgtca gccatctccg tcataatcga
     4081 gcccgggtct agtgcgaatg ctttcacacc gtattctttt acctcgttgg ccacaagttc
     4141 cgccagcctg ttctgcgcgg tcttaccgag gctgtaagcg gaaagattcg ccgtcagcat
     4201 atggcaacgc ggcgaggaaa tattgatgat gcggccggcg cgccgttcaa tcattcccgg
     4261 cagcacggcg gacatgaaca aaaatggcgc caggatgtgc acttcctgcg ccgcccacca
     4321 cgcctcggga tcgagcacgc ccaccgggcc gtaggggccg ccgatgccgg cgttgtttac
     4381 gaggattgtc gcgccgccca gcttttcgac ggtcgctcgc gccacgcgcg cgacatctgc
     4441 tgccttggtg acgtcgcccg cggcggccag tcctcttcca cctgcgccct cgatttgcgc
     4501 gagtacctga tcgagctgcg cctgattgcg tgaagtgacc gcgatggccg cgccttcaga
     4561 cgcgaatcgc agcgcgatgg ccttgccgat tccccggccc gcaccggtga cgatcgcgat
     4621 ttggccctgc agcagtcctg ccatcagatc gcttactgca gctcttccgc ggtcacccag
     4681 ttgccgtgca cctgattgcg caatcgcaac ggcaggtgcg cggtcgcgat cggccccttg
     4741 tcaaggtgtt cggcctcgaa caccagcaag tctgataact gcgtatcgtg cagatcggcg
     4801 accagcgcga gataaccttc gtgccccggc gtcctcgatg ggatgtgaac ttcttcctgg
     4861 atcgtcactg ggcgcgttgg acgccacgac ttcatcttgc cgctttttat ttccaggcgg
     4921 ctgagcgtat tgaatcctgc gcccaccggc cccgcaacca tgggcgggcc aacctcagga
     4981 tcgaagcgct cgtagtagcc gatggagtaa tccttcatat ggtctttatt cgcgatgcgc
     5041 ggaaaatcgc cggagggcgc gagtttgtac tcctcgaatt tttctcctgg cttcgacatg
     5101 tcgaatgtcc agcgcaccac gcggccatat gcgaaatcct cggctttcgg atggatgtgc
     5161 gatgcctctt gaatgaacgg gaacggcgcg tatttgccga tgccgaaatc caaatgcaca
     5221 aatttgcctt ccgtatacgc gttcatgaaa tgaaacgcgg agcacgcggg gtggcggaac
     5281 cagcgcatgt ctttggtgtg gccgtcgatc ggcatgattc cgacatacgc atccttttcc
     5341 ggctcgaaca cccaatgcgt gccgccggcc ttcatgcgat ccagatcggc agtggtggga
     5401 aacacgggga agatcacgtg ctccttcgat accgcgaaat cgtgcatgag agaaacgtag
     5461 ggaacctgga accattcctc ttttaccagc tggccctctt tgtccgccac gcagtaggaa
     5521 acgtcgcgcg tggccaggcc gctggcttcg tagccgaaaa agtg
//
LOCUS       DATNDT010000157        35319 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1431995, whole genome shotgun sequence.
ACCESSION   DATNDT010000157 DATNDT010000000
VERSION     DATNDT010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 35319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35319
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1431995"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..2632)
                     /locus_tag="VN788_05010"
     CDS             complement(146..2632)
                     /locus_tag="VN788_05010"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19924.1"
                     /translation="MYWLQSMGSDVRYALRMMRRTPGLTTTAVVSLALGIGGTIAIFS
                     VMYAFVLRPLPVLQPDRLVEVADPGGSNFHSYLEWKEFQRLQNLFSGVFAYNDLDRSF
                     SLAEGDKRENLEGLYVSGGYFSALGIKAILGRTLQPYDDEPGAAPVCVIGYGLWRTQF
                     NQSRSVIGRTLILNGHAFQVVGIAPGSFFGVDIGQRPQLFMTLESERIYKDYQIYEGH
                     PTPSLDDPHPAYLLLTMFGRLKPGVSLSQASARLQVLGAEVYRAIPPIIDSSTGQRYV
                     GTYIAHPIFSYAWGDHRDTLLLLMLMAGIALFITCANLGNLQLARMTKRRGEIAARLA
                     LGATRWRLMRQLLTESVALALAGGVAGLFVAHWGSQALLSLISNPNDPTVLDLSWDAK
                     LVAFAVGITVLCALLFGLAPAIRAANLPLYSAINSGASGGKGRNRFSNAALLMVQISL
                     SIVVFVGAGLMVRTVHALLSVDLGYQARGVLAIQADWPQTANNARQAAAASQLLTAFR
                     SLPGVVSASGVISYLGGARPTMVVLQAGGYQRSVPGIGLFISSDYFRTRRTPLLGGRD
                     FNDGDQQGSLRVAILSGFAARTFFPGMNPVGMRYEERAQTGDNAGQEYPVEIVGVAKD
                     INERPANYAPMPIVYRPVSQCGTQCSAMSTFEVRYTGPLHDVTSQLKDSAANIDSHVP
                     LKFSLLTDHILSGIRRNRAREFLATFFGFFAGLLAMIGIYGVTSYAVAERTREIGIRI
                     ALGAQPRRVFRMVLGETTIVVFVATALGIAGAYDVAKAMRGILWGISPNDPLSFVIAA
                     CLMLTIVEVAAFVPALRASRTDPMISLRCE"
     gene            3342..4046
                     /locus_tag="VN788_05015"
     CDS             3342..4046
                     /locus_tag="VN788_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19925.1"
                     /translation="MFHNRIARQSTGTDIHGREDVFIRLPETIPNPRLAMLARPGLAK
                     TLLGMGPRFLLMMYPRSNIYRALNVNPGTGIYQDAERIYARNLEVPSGNGVGTARAIA
                     RAYSVFAGDGHELGLRRETLELLAAPAIAPACGFYDECMKARDVRFSLGFMKPGSVWR
                     FRNTESFGSPGAGGALGFADPAVGVGYAHVTSQMGTRLTGDPREMALRNALYSAIPVH
                     GKQPTACENLAKRVVA"
     gene            4169..6094
                     /locus_tag="VN788_05020"
     CDS             4169..6094
                     /locus_tag="VN788_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008702050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXU19926.1"
                     /translation="MEPTVKTPPSCSQPNVAQRQLSQKPLTGFWHGKTTVIAAFSVAA
                     ILLDLVLRFGFHATPRIYQTPLLAALIIGGLPLLYDLLRKLLKREFGSDLLGGISIIT
                     SILLGEYLAGSIIVLMLSGGDALESYASRSASSALVALAKRMPSIAHRRRIAEVLDVA
                     LAEIAVGDTLVIYPHEICPVDGVVTDGHGVMDESYLTGEPFKITKTNGSTVISGAING
                     ESALTIRTTQRAADSRYAKIMAVMRESEAKRPQLRRLGDQLGAAYTPVALAVAILAWV
                     ISGESIRFLAVLVIATPCPLLLAIPITIVGSISLCARRAIIVKSPVVLEQIARCRTAI
                     FDKTGTLTYGEPRLAEQLVTPNFARREVLTLVASLERYSKHPLARAIIASAKEEALEL
                     PEATEVSEPPGQGLRGTVSGHQLQITSRAKLAGQKIAGLDQLPQVGGGLECVVAIDNR
                     YAASLRFRDAPRAESRSFVKHLGPKHQFQRVMILSGDRESEARYLAEQVGITEIYAQK
                     SPEDKLAIVRKETAVAKTLYVGDGINDAPAMLAATVGMAIGQNSDVTAEAAGVVIMDN
                     SLEKVDEFMHISRRMRSIALQSAVGGMALSVIGMAFAAAGLLSPVSGAISQEVIDILA
                     VMNALRAAFAPKVIHDL"
     gene            6165..7610
                     /locus_tag="VN788_05025"
     CDS             6165..7610
                     /locus_tag="VN788_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004999357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXU19927.1"
                     /translation="MSDEILKELQREARVPNRIVADQAFSRAAGAPLIPGNRIRLLRD
                     AAENYPAWLDAIRTARNTIHFESYIIRSDEVGCQFRDALAAKAREGVRVRLIYDWMGA
                     LGHTHYPFWRSLKSAGIEVRCFNPPRLDSPLGWLRRDHRKMLSVDGRIGFVTGLCVGR
                     SWVGDSRRGVEPWRDTGVEIQGPAVADIEHAFAQMWMILGSPLLTNDLWDGKDARPEG
                     DVALRIVPSYPNMGGLYRFDQLIGALARQTIWLTDAYFIGTASYIQSLRAAALDGVDV
                     RLLLPRINDVPIVRTLSRAGYRTLLEAGVRIFEWNGSMLHAKTAVADGRWARVGSSNL
                     NVASWLGNWELDVVVEHEGFAREMEQMYLNDLVYSTEIVLSPNQKVRSAGQTSRQPHH
                     LKITEGSGGRAATGLLRVGNTIGAAITSRRGLGPAETGVLAIAAALLLCVAIIGILWP
                     PTISIPVVVFCVWVVLALFARIYRLRMNQDR"
     gene            7713..8516
                     /locus_tag="VN788_05030"
     CDS             7713..8516
                     /locus_tag="VN788_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HXU19928.1"
                     /translation="MIKADYTNRIRVATYNIHKCRGLDGRVRPARILEVLREVDADII
                     ALQEVVRIEADRREKDQANFMASELGMNFHFGENRQLNGGTYGNALLSRFPLRASCNY
                     DISTSDREPRGCLRVDMNLPGGSLLHVFNIHMGTAFLERRKQALKLVSPKILHQPTLS
                     GTRIVLGDFNEWTRGLTTRLLRQHFVAPDLRDHMEHSRTYPGVLPFLHLDHVYFDPTL
                     RLDHLTVHKTRASLIASDHLPLVADFSLTAWTKDSTSAKTSDKRSEVAA"
     gene            complement(8606..9466)
                     /locus_tag="VN788_05035"
     CDS             complement(8606..9466)
                     /locus_tag="VN788_05035"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXU19929.1"
                     /translation="MKEHKKLGNILFATDLSENSARAIGWLRWFCGNFGSSVDIVYVL
                     NQFPFSVSAEETAQARSVASRQFDGFVRKHHLNQKPFAQKLIVGDAAVAVSEFAGKHD
                     ISLVVLGSQAIGLNRLLQGSISEEVFRSVDCPVLTVGPRAKSPRGQDGISHVLFATNL
                     EKRSESVFPHFEFLFKGNSRPELALAHFLGKESKSVVERHKTRKRLRGKLINLVPEEV
                     RSQIDDVVVESCSPVNGILELSREGRFDLVVLGVKDARPFTRAATHRPRSITHQIIQH
                     ATCPVLTIRV"
     gene            complement(9553..10620)
                     /locus_tag="VN788_05040"
     CDS             complement(9553..10620)
                     /locus_tag="VN788_05040"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXU19930.1"
                     /translation="MNRRNANIVVLLILLAIGIIVAYGILRPFLRPIVFALVIGIGFY
                     PLHVRIGRSLSQTNAHALISTLAVFLIFVVPAVLLVSAASGEIIHAAKSFNDRAAEGG
                     GLLPYFLQGPDRLMNWLEKYVDIKKSGLAGAIDSLPIKASQLLVTFATSLVKGLAGFV
                     GESVITLLILFFMFRDGPDILNRVVAMLPLDRDRTRQLFSQVRESVFANLYGILAVAL
                     AQGILTSAGVAIVGVGSSLLLGIAAAVCSLIPFVGPTLVWLPLVIFLFVKGHWWKGLF
                     LLIWGAIAVGTADNIIRPLVITTRVKLHPLLLLFSLIGGVQEFGFVGLFIGPVVMSVI
                     LSLVSMLREEFGDTNGKPASA"
     gene            10873..13608
                     /locus_tag="VN788_05045"
     CDS             10873..13608
                     /locus_tag="VN788_05045"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017301653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HXU19931.1"
                     /translation="MSDFYQHDSADVLTQLNVNASRGLTEAEAAHRLKKYGRNELVGM
                     GIRSPWGILWGQLTALMVLILIGAAVVSALMADYKDAIAIAVIVVLNTLLGFGQEYRA
                     ERAMAALQKLSLPFVRVRRDAEIKNVQSPQLVPGDIVFFEPGNIVAADCRVFESTYLQ
                     TQESVLTGESQPVQKIASALNQPNLPLGDRHNMVYMGTSVTAGRGQAVVTATGMGTEL
                     GRIASLVQAVNREPVPLQRRLGHLGRRLAVIALLLVAVIFTLGLLRGGDLKLMFLTAV
                     SIGVAAIPEGLPAVVTIALTLGAQRMLKRKVLIRRLAAVEALGSVTVICSDKTGTLTE
                     NRMTVATLNLANRVLELPGNLRNRAVERPPVEEMRDSGFKLLLGGAALCNDAIEQRND
                     QQPGSAVILGDPTETALLTAARNLDLSKTDLERLMPRVEEIPFTSERKRMTTIHPLPT
                     GPPPIPIEIATPNNSGGLSYVAFTKGAPAELLRVSDTVWMDGKIEDLSESLRQHLGQA
                     TDDLAGKGMRVLGIAFRYMGSLPASMDDEAIERELTFVGLVGMIDPPRSEVVAAVATC
                     GAAGIRPVMITGDHVLTAKYIADQVGITDEGESIAGPELDHLSNDQLKSVAGRTSVYA
                     RVSPEHKLRIIQALQERGEVVAMTGDGVNDAPALKRADIGVAMGVTGSDVAKETADMV
                     LLDDNFTTIVAAVEEGRVIYDNVRKFVRYILATNSGEILVMLVAPFLGMPLPLLPLQI
                     LWMNLVTDGLPALALAMEPAESDIMRRPPFAPQESIFARGLGSHIVWVGILMASLSTG
                     VGYFYWRADRPNWQTMVFTTLTISQMAHVMAIRSERNSLFRIGFLSNKPLLGAVMLTI
                     ILQLSLIYAPVLQGVFRTSALSPLDLGLSILLSGWVFAAVEFEKWLFRVQRPDPR"
     gene            13961..14533
                     /locus_tag="VN788_05050"
     CDS             13961..14533
                     /locus_tag="VN788_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19932.1"
                     /translation="MSKMRIGIRGTTAVSILALLSVLFLSAAPAVLASPRPKDSEQVT
                     KLLTNVKSEAVQLKDDAEDMKSFTWSKQLSWQSHAAKIEQIKQHVNASGKLLTDLQNS
                     KASASAWQGQAIDRIAPILQELAASVSSTIEHLNKNQDRIHTAPYTDYVASTADLASD
                     LSELITDYVAYGEAKNRSENLARKLEVPGA"
     gene            complement(15364..16047)
                     /locus_tag="VN788_05055"
     CDS             complement(15364..16047)
                     /locus_tag="VN788_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXU19933.1"
                     /translation="MKTDKQLQQDVLDELVFEPSVDAAEIGVSVENGVVILNGTVKSL
                     TEKWTAERVTQRVEGVRAVTDELTVKLAGDGKHNDQDIAQAAVNALDWNASVPPKRVK
                     VLVENGWVTLDGSVEYHFQKAAAEGAIRNLKGVKGVSNVIVIKPRVSAEDVIHAIKKA
                     LHRAAQVDAEKIAVEASAGTVILRGNVRTWAEREEAERAAWAAPGVSNVQNDIRIVSA
                     VGAQSATAK"
     gene            complement(16104..16622)
                     /locus_tag="VN788_05060"
     CDS             complement(16104..16622)
                     /locus_tag="VN788_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXU19934.1"
                     /translation="MTANATATQMARVPTKALGPRDVFDRFEQIYNLIARRAFEIFES
                     DGRSFGHELDDWFKAESELLHPVHVRIAESDEALTVEAEVPGFEAKDLQVNIEPLRLT
                     ISGKKETKEEQKKGKAVYQEQCSNEILRVIDLPAEVVASKATATLKNGMLELQMPKNP
                     KTKGTHVEVTPA"
     gene            16840..18465
                     /gene="groL"
                     /locus_tag="VN788_05065"
     CDS             16840..18465
                     /gene="groL"
                     /locus_tag="VN788_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011087406.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXU19935.1"
                     /translation="MPFKRLLFKSAAREKVLKGATALTEAVRVTLGPKSKCILIEKKW
                     GRPIVCNDGVTIAKEIEFEDPEENLGAKMIREAAERTGDAVGDGTSTSAILAHAIFAE
                     GLHNVAAGASAVELKRGLDRGLRAAVESLHGISRPVSSRLEKAQIATVSAHNESTIGE
                     LVADAMEKVGKDGVVTIEEAKTTETALEVVEGMQCDRGYLSPYFVTDPEKMEVILEDP
                     VILIHEKKITNLQEFLPFLEKVAKSGRPLLVIAEDVEGEALATLVVNKVRGVLFSAAV
                     KAPGFGDRRKEILQDLAVVTGGKVLAEELGVKIENLELDELGKAKRVIIDREKTTIVQ
                     GAGLKKAIQGRCNEIRKQIDQTTSDYDKEKLQERLAKLSGGVAVIRVGAPSETEMKNR
                     KEAFEDAISATKAAMAEGIVPGGGLVLLRAIDAVQRAESECSGDERTGLVILKRALEA
                     PTRQIAENSTLDGGVVVDRMRNGAGNFGLDAAKGEYVDLVAVGIIDPTKVVRTALENA
                     VSVAGVLLLTEATLTEVPEPKMGRTPSHDMPLE"
     gene            18552..19304
                     /locus_tag="VN788_05070"
     CDS             18552..19304
                     /locus_tag="VN788_05070"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HXU19936.1"
                     /translation="MANRLTGKVALITGAARGMGEADARIFAQEGARVIVADVLDTEG
                     QRVAGEINHAHPSCAEYVHLDVTLASDWENAVATAERSFGGLHILVNNAGILSFAGVE
                     QTTEAEWQRIVDVNQKGVWLGMKAAAPALRRAGGGSMVNISSIYGLIGSGGAAAYHGT
                     KGAIRILSKTAAAEFAPANIRVNSVHPGIITTPMVEPLAQDLRKNIASAGCIKREGRP
                     EEVAYGVLFLASDEASYVTGAELVIDGGYTAL"
     gene            19537..19680
                     /locus_tag="VN788_05075"
     CDS             19537..19680
                     /locus_tag="VN788_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19937.1"
                     /translation="MLPLWQLDVVEPDTTWKPWDQKKVNRLLETGWRLLHIYTRKYEE
                     DGV"
     gene            19801..20022
                     /locus_tag="VN788_05080"
     CDS             19801..20022
                     /locus_tag="VN788_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HXU19938.1"
                     /translation="MKSRLQITFRNMEPSKEIEEWIRAEAAKLDLLYNQVMGCRVMVE
                     IPHRHHRNPYRFTCSLSAPVSAWGDPPAA"
     gene            20049..20192
                     /locus_tag="VN788_05085"
     CDS             20049..20192
                     /locus_tag="VN788_05085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19939.1"
                     /translation="MMQFSLNVLIQLECWQGKRFVSEVLEIRGHDPEADHYDLYPIFA
                     AQS"
     gene            complement(20202..20582)
                     /locus_tag="VN788_05090"
     CDS             complement(20202..20582)
                     /locus_tag="VN788_05090"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HXU19940.1"
                     /translation="MRKRKSSEVDGYIATCPKEAQGDLAKIRAAIRAAAPGAAERTDY
                     FQIPGYSYPGFDYDGMFAWFSFKKPHIRLHVRPPVIQEHKKELAGYATTKAIVSFPLG
                     KPVPMTLVKKLVKASLKAMKDKSE"
     gene            20727..21164
                     /locus_tag="VN788_05095"
     CDS             20727..21164
                     /locus_tag="VN788_05095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU19941.1"
                     /translation="MTLASKSVCDEETVNNAGKLEAPSNQILLACILLLPKDAAPLLA
                     LLKQVNGNSGPSTTGQIADVRTMNSPRPQSESWLAHLEAADYLGISKSTLYRFACEGS
                     IGRSGTSCRGMIQPSFQIRAKAPSSIGPSRQGSWFGLRLCSLC"
     gene            complement(21181..22374)
                     /locus_tag="VN788_05100"
     CDS             complement(21181..22374)
                     /locus_tag="VN788_05100"
                     /inference="COORDINATES: protein motif:HMM:NF037429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19942.1"
                     /translation="MVTMSQLESFSNLPPPLLTAYVRTVSVEAFLHGATPEYLAWLKD
                     ESRPVAESLPPTEQELFRKQLDRVEEFLRQRQPHERSLVIFAGPAVWEIASLQREVEN
                     ELHWGKPALTQLLWHASEHKPYGIVAVDHAGARFFHYWLGEMVEHEERKFAIDTSQWK
                     NKELSHVVDSGFKGRLRPIPAGGRGAQRDIFKKRMESQYAHLCHETAQEAVIFCKKKD
                     LAAVFLVGLDRLIRPMVAAFPRGFRQPIVLVHQDLARITLLEFQKHLEPQIASWERTH
                     EAEIVAAVADEERGTIAGFDEILAHLQKGKIRTLVLARGLDADLRECVQCGWMDRSAD
                     PTCSICGSKRRKVTLRDALPELARRHQVEIDVVSGDAAERLNKVEGIGAWLRQPKSAS
                     ASGKR"
     gene            22582..23028
                     /locus_tag="VN788_05105"
     CDS             22582..23028
                     /locus_tag="VN788_05105"
                     /inference="COORDINATES: protein motif:HMM:NF024304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HXU19943.1"
                     /translation="MLIQEMTKQESLDLLARTRLCRLGCVQGNQPYVVPFYFAYQNNS
                     LYGASTVGQKIDWMRKNPLVCVEVDEVESPQKWISAVVFGRYEELPDTPEYHRERAVA
                     HNVLQKQRMWWEPGYVKTILHGTERPLVPVFYRISIGHITGHRATP"
     gene            23133..23738
                     /locus_tag="VN788_05110"
     CDS             23133..23738
                     /locus_tag="VN788_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXU19944.1"
                     /translation="MRVFPINCPLTRFESFRTKMLAKDLMTSAVICCTPWDTAQKAAS
                     LMKAYGVGAIAVVAGTLDPLLEGIVTDRDLCCGVIAAGKNLDAVQVANIMTRIPVTCG
                     PEHTLDECLELMQENQVRRIPVVNPRGRCIGIVCQADIALHAPAAQVAKTIKEFSQPA
                     KPCLEMYFEKGYFYCGQPHEQDQILLLNRRRELKHKHEVYV"
     gene            23735..24430
                     /locus_tag="VN788_05115"
     CDS             23735..24430
                     /locus_tag="VN788_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016875095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HXU19945.1"
                     /translation="MIFANRTEAGQELALRLRTYANRDDVIVLGAPRGGVPVAFEVAT
                     ALKAPLDVFVLRKLGVPGREELAFGAIASGGVRILDRDTIESLGITGLDIERVTRVEK
                     QELERREQSYRMGRPAPDVSGLTVILVDDGIATGSSMRAAIRALRQMKPARIVLAVPV
                     APPSTCSQLQFEVDELVYLEMPEPFYGVGRFYDDFTQVSDEDVKELLDEASRQRRAPH
                     YQGVRTITPGTQQ"
     gene            24515..25846
                     /locus_tag="VN788_05120"
     CDS             24515..25846
                     /locus_tag="VN788_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014394589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin esterase family protein"
                     /protein_id="HXU19946.1"
                     /translation="MVPKHEHDLIKAIRQAAHPLTGETADFDPLLKMIGDSRFVLVGE
                     ASHGTHEFYRLRAQLTKRLIAEKGFTAVAVEADWPDAYRINQFVRFDGEDEDAVDALA
                     GFRRFPAWMWRNADVLDFVGWLRNYNEHRHSQRVGFYGLDLYSLHASMRAVLDFLDKV
                     DPEAARRARYRYACFENFGEDTQAYGYAASFGLSKSCENEVVSQWVEMRQRAADLARR
                     DGHVAPDAFFFAEQNARLVKNAEEYYRSMFHERTSSWNLRDSHMAEILDALTHHLGAK
                     SKIVVWAHNSHLGDARATEMGQKGELNLGQLVRERYDKEAILVGFTTYTGTVTAASGW
                     DSAAERKHVRPALPGSYEALFHETDIQNFLLTLDRKEKVASLLREPRLERAIGVIYLP
                     RSERASHYFHAQLPDQFDAILHFDETRAVEPLERTAAWETGEVPETYPSML"
     gene            complement(26102..26590)
                     /locus_tag="VN788_05125"
     CDS             complement(26102..26590)
                     /locus_tag="VN788_05125"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXU19947.1"
                     /translation="MKVTDVMAKPPAYCSPQTNLGSAVEILWRQNCGILPIVDSKEEV
                     VGLVTDRDICVALGTRNRLPSELTVSEVTSGKVVACKPDDDLRAALAIMAQARVRRLP
                     VIDAAGKLQGILSIDDIVVRTETETPQGDSELSLAELANTLKIVYAPRPLRAVRQKAA
                     AA"
     gene            26798..27055
                     /locus_tag="VN788_05130"
     CDS             26798..27055
                     /locus_tag="VN788_05130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19948.1"
                     /translation="MGVQFWRGALPRSPSPEIKTIQERLGHASAGSLTLDVYTHSEWS
                     EKVEAAQVSGEQIEKAVNSVSLTAIEEKGPADRNQQALQYA"
     gene            27547..27903
                     /locus_tag="VN788_05135"
     CDS             27547..27903
                     /locus_tag="VN788_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXU19949.1"
                     /translation="MRDALRQFKSEIFQGLAHPTRIAIVELLREGELSAGSLIEKLRI
                     EQANASQHLAVLRAKQIVVSRKVGNQVYYSIRDRALIEVLDILRRYFYAQLDNTVNML
                     REVASEQPKSRLRTNR"
     gene            27900..30725
                     /locus_tag="VN788_05140"
     CDS             27900..30725
                     /locus_tag="VN788_05140"
                     /inference="COORDINATES: protein motif:HMM:NF021578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HXU19950.1"
                     /translation="MTDCSPPRLARLDSLRRAVEQAAHLLPMQGPIGVFVHHNTLHAF
                     EHLPFEEAVIQAAQLFDAEPYMSEAAYRAELSRGRIQFVDVDAVLDREPDSLIFARLS
                     RSSLRRAMITPGVREFDGATIIWRTEQGDLAKDFRRPALRALFDACFARAVAHEEEPT
                     PPRPVDEVIHPWLIRLCSVFLDQGTAYWSMPNREKGFYECVRALFLCRGGMFPRYLVG
                     LDDEFGKQECLSFSASDAVLDYLDSQSFREAEWPEIVKAELLALPGWAGLMHRLEKDP
                     SLAPHKTLPCSLMDLLAVRLTMSRVAQRRGAAETVSQESPLKTQEKRRLSRSARIYDA
                     ARVLRLSAGEINRLSESEWSALVNEVKAFNGLERRRILHLAYERWHEREILRGLASHR
                     KYRGLFDQRGRPPAQVFFCLDEREESMRRALEEADPRVETYSAAGYFGVAVDYKGIDD
                     PHGAALCPVVIKPEHAVVERPKAEDSVLLESRRWRRRILGLLMRNSFVSSKTLLRGWL
                     STSALGLLSAVPLIGHLLAPRRYALLRDWLNREFLPEPRTELTLMRNTKESQNVVTGL
                     LTGFAPGEKAERVASVLRPAGLARGFARLVVILGHGSTSLNNPHESAHDCGACGGRRG
                     GPNARLFAAMANRPEVRERLREEGIDIPGDTWFVGGYHDTCSDDVELFDLDAVPATHQ
                     SDLASIRKSLDQARAQNAQERARRFESSHSEADPRRSLRHVEERSEHLAQPRPEYGHC
                     TNSVCIIGRRSLTRGLFLDRRAFLVSYDASQDRDDHSLSALLSAAVPVCAGISLEYYF
                     SFVDNDKYGCGTKLPHNVTGLVGVMDGHASDLRTGLPWQMVEIHEPVRILFVIETTPN
                     RLDKVINASASLEQFVENRWIRLATIDPISGRVHVQRDSGYEEFHGALERLPVALSSP
                     EWFAGKLQHLPMAWIQAPQTPAGDSMR"
     gene            30722..32062
                     /locus_tag="VN788_05145"
     CDS             30722..32062
                     /locus_tag="VN788_05145"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXU19951.1"
                     /translation="MSLSQAILVFVVLAPGVVFAAFALLWLLGWTPGERFLSSITGLT
                     FVACTFGIASLVWSVASGSTVTVSFGNWFAVGDYHFPLVLMADRLSLPFLALTVVLSG
                     LIAQFSATYLHREPGFLRFFLLLHLFAFGSLLAFAAGSFDLLTAGWEVVGITSVLLIG
                     FFQFRPAPVENGLRVFGIYRACDIGLFVGVFALHHWAGTASFESGLPALTGTQAVIVC
                     LLLLLAAAGKAAQIPFSGWLPRAMEGPTPSSAIFYGAISIHAGAYLLLRAQPLLARSI
                     LASALVILIGLATAIHGTIVGRASADAKTSLAYATLTQVGVVFIEIGLGWKWLAVAHI
                     LGNATVRTMQFLRAPSMLHDYHQMHSAIGGDVSPTGKQIEELLPERVQLWLYRWAFDR
                     GHLDTILDCWVIDPLLQVSTYLAKADRVGMRLTAIRRGNAVPISLHGPAKQGND"
     gene            32068..33573
                     /locus_tag="VN788_05150"
     CDS             32068..33573
                     /locus_tag="VN788_05150"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HXU19952.1"
                     /translation="MPGWVLHQPLLIGVSVGVPWFAAALLTGYRHHARIVGCAAAFVS
                     ILGSAVLLADAPKGESLYQALMLLYSCLTFGATLLLPRRDCTTGTISGILFLLGSTLL
                     AYSTGNLLVLLAAWILSTVPFLWAGWFRARAWRPLAGLLLSSLALALAVGLISTSAGV
                     FSIDNLKGQSPGGVAAFALLLIAVVFRKGICPAHSWVADAADTGPVVPTALLLNGHFG
                     ALLVAKLIVPLYPRTDSLFPVLSYLAIVTAVYVAIRALAENSPSRLIAFLALSQSAYI
                     LSGLESRTAEGIAGAIVHWFVVAISTMALFGILRLLEVRFGENLTVSKHFGLAEHAPR
                     LAVSFVIFGLALVGLPGTLGFCSQDLLIHGALTSHRLIGLLLPIATAMNAISFFRLFT
                     RLFLGKRRTGFTVMADALPRERWILAAGVLFVVFGGVFPNAVVIQRSSEADMVQEAIH
                     TAAELNPVGKVNRHPGNYQSLPRADAGPMAAEAHGRALSWVLRTTFVGASR"
     gene            33570..33791
                     /locus_tag="VN788_05155"
     CDS             33570..33791
                     /locus_tag="VN788_05155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19953.1"
                     /translation="MRNDKFGTHLTSGNGTGRKDAVARGLEKQQEHTRNLRFGIFYLV
                     VGLLLFLAINLQSSRRRTVTSPTRSRTSA"
     gene            complement(35116..>35319)
                     /locus_tag="VN788_05160"
     CDS             complement(35116..>35319)
                     /locus_tag="VN788_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19954.1"
                     /translation="VKRNLPQTPKNFRAQTLWMRGRKLSREPFQKRTIVEASRADCGR
                     HAPPNYPAPIARGTERGKASEIP"
BASE COUNT         7749 a         9765 c         9879 g         7926 t
ORIGIN      
        1 acctgctgct cttcctgcag gccaagaccg tctccgagaa aatggacgca gctagatagc
       61 ctaagcgtca ttccggttct tttatgctaa ttgcgagtca tggctaactc cccgaataag
      121 aatcctcggg cagcaaagct ctatcctact cacatcgaag cgaaatcatc ggatcagtcc
      181 tcgatgcgcg aagagcggga acaaacgcgg cgacttcgac gatcgtgagc atcaagcagg
      241 cggcgataac gaagcttagc ggatcgttgg gcgatatgcc ccacagtatg ccacgcatcg
      301 cctttgccac atcataggca ccggcaatgc caagcgcggt ggcgacaaag actacgatcg
      361 tcgtttcccc aagaaccatc cggaacacgc gacgaggttg cgctcccaaa gcaatgcgaa
      421 tgccgatttc gcgtgtcctc tctgcaactg cgtacgaggt cacgccgtag atgccgatca
      481 tcgccagtaa tccggcgaag aagccgaaga aggtcgccag gaactccctc gcgcggttcc
      541 ttcggattcc gctgaggatg tggtcagtga gcaggctaaa tttcagcgga acatgcgaat
      601 caatgtttgc ggcggaatcc ttcaactggc tggtgacatc gtgcaacggg cccgtatagc
      661 ggacctcgaa ggtgctcatc gcggagcatt gcgttccgca ttgcgatacg ggcctgtaca
      721 cgataggcat tggcgcgtag ttggccggcc tttcgtttat gtcttttgct acccccacaa
      781 tttcaaccgg atattcctgc ccggcgttgt ctccggtctg ggcgcgctcc tcgtatctca
      841 ttccgacagg attcatgcct gggaagaatg tcctggccgc aaagccgctg agaatggcga
      901 cgcgtagtga tccttgctga tcgccatcgt taaaatccct gccacccagc aaaggagtcc
      961 gtctggtccg gaaataatcg gaggagataa agagtccaat acccggaacg ctgcgttggt
     1021 aaccaccggc ctgtaaaacg accatggtcg gccgagcacc gccaagatac gagataacgc
     1081 ccgaagccga tacaacaccg ggaagcgaac gaaatgcagt gagcagctgg ctagcggccg
     1141 cagcctgccg ggcgttgttc gccgtctgcg gccagtcggc ttgaatcgca agaacccccc
     1201 tggcttgata gccaaggtcc acggagagca gcgcatgcac ggttcgcacc attaagccag
     1261 cgcccacgaa aaccacgatg gataagctaa tttgcaccat gaggagcgcg gcattcgaaa
     1321 accggttccg acctttcccg cccgacgctc cactgtttat ggccgaatac aacggaaggt
     1381 tcgccgcgcg gatcgcaggg gcaaggccaa atagcagcgc gcacagcacc gtgatgccca
     1441 ccgcgaaagc gacaagcttg gcatcccatg ataggtccaa aaccgtgggg tcgttggggt
     1501 ttgaaatcag ggacagcaag gcctggctcc cccagtgcgc tacgaaaagg cctgccactc
     1561 cgcctgcgag cgccagtgca acgctctcgg tgagcagttg ccgcataagg cgccagcgag
     1621 tcgcaccgag cgcaagccgc gcggcgattt caccgcgccg cttggtcatt cgcgcgagct
     1681 gcagattgcc taggttggcg caggtgatga acagagcaat cccggccatg agcatgagca
     1741 aaagcaacgt atcgcggtga tcgccccacg cgtaagaaaa tatcgggtgg gcaatatagg
     1801 tcccaacgta gcgttgccca gtcgaggagt cgatgatcgg tggtattgcc cggtacactt
     1861 ccgctcccag aacttgtaac cgcgcgctcg cctggctaag gctaactccc ggttttaacc
     1921 ggccgaacat ggttaacaac aaatacgcag gatgcggatc gtccaaggag ggggtcgggt
     1981 gcccttcgta tatctgataa tccttataga tcctctccga ttcgagcgtc atgaatagtt
     2041 gtggtctttg gccgatatcg acaccgaaga atgatccagg ggcaatgcca accacttgga
     2101 acgcgtgtcc gttcaatatg agtgtccggc caattacgct tcgggattga ttaaactgag
     2161 tccgccacaa cccatagccg attacgcaga ccggcgccgc tccgggctcg tcatcgtaag
     2221 gttgcagcgt tctgcccaat atcgccttga taccaagagc tgagaaatag cccccgctga
     2281 cgtacagccc ctccaggttc tcgcgcttgt ccccctccgc cagagaaaaa gaacggtcca
     2341 ggtcgttgta agcgaagaca cccgaaaaga ggttctgcag gcgctggaat tccttccatt
     2401 ccagatacga gtggaaattg gacccgccgg gatccgccac ctcgaccaag cggtcaggtt
     2461 gaagcacggg caaaggccgt agaacgaagg catacatgac ggagaaaatc gcgatcgttc
     2521 cgccgatccc cagcgcgagg gacacgacgg cagtggtcgt caaccccggg gtccttcgca
     2581 tcattcgaag cgcgtaccgg acatccgacc ccatcgactg aagccaatac attttcagcc
     2641 ccttaccatg tggactcaat ccacctcaga acaggctcta ggaatcggca tcctcctggc
     2701 ggatcgccca tttcccgtgt acgccaggta atcatcgctg cggtcagata gcagcaacgc
     2761 gcggtcccac tgcggttcta tagtcgtgac cgccgcaagc aacgccagtg cgattccgat
     2821 cgacccgtcc aaaaaggcgg gccacggttc ccaaccgaga ttaccatcct ccctagacac
     2881 acacttcgag tagccaccga agccagttcc ggacttaaac atttcaagcg cgcgcttgta
     2941 ccaaaccagg gcggcgcctc ggaagcgctc acctccggtc gcctggtata ttcgattgta
     3001 gatgtgcgct attccgaccg ccccatgaca caggccagca tcttcgatct gatatgatcc
     3061 aacgggcaac ttaacgcacc tttccagtac cttccgtccg aatgcaccca cgtcacggcc
     3121 ggcgcgcccc ttcccaactt gcacgagtat ggcgccgacg cccaaatccc catagcacca
     3181 cacgggcctt gagctctccc agtttccgtc cggagacacg cgcgaaccga atcgtgcgcc
     3241 cctcctgttt tggtcaacct gatcgcgagc gcctatctgt cgcattcgcc gtggtgacct
     3301 gtatcacagc cgaatcccga tgccagacat agaagtagct tatgtttcac aatcgaatcg
     3361 cccgacagtc cactggtacc gatattcacg gccgagaaga cgttttcatc cggttgcctg
     3421 agaccatccc gaacccccgc ctggcgatgc ttgcgcggcc gggcctagcc aaaacactcc
     3481 ttggcatggg acctcgtttt ctgctgatga tgtatcctcg ctccaacatc tatcgcgcgc
     3541 tgaacgtaaa cccaggcaca ggaatttacc aggacgccga acgcatctac gcgcgcaacc
     3601 tggaagtgcc gtctggcaac ggcgtcggca cggcgcgagc tatcgcccgc gcgtatagcg
     3661 tcttcgctgg cgacgggcac gagttgggtt tgcgcagaga gactcttgaa ctgctggctg
     3721 caccagccat cgctcccgcg tgcggctttt acgacgaatg catgaaggcc cgcgacgtcc
     3781 ggttctccct gggtttcatg aagccgggct cagtttggcg gttccgtaac accgagtcat
     3841 tcggatcacc tggagccggt ggggcgcttg gttttgcgga tccagctgtc ggcgtcggct
     3901 acgcgcacgt aacaagccag atgggaacgc gattaaccgg cgacccacga gaaatggcac
     3961 tcagaaatgc cctctactca gctattcctg ttcatggcaa acagccgaca gcttgtgaaa
     4021 accttgccaa acgtgtcgtt gcctaacttt gtggtggata gaacacggat acccggcagc
     4081 agtcgggtgt ccgagcgcgc cgagtcgtat ctgaacgcac gcaccgcagc gtcgaagacc
     4141 tcagggattc aatatgtcgt ggtaacttat ggaacctacc gtgaaaaccc cgccctcctg
     4201 tagccagccg aatgtcgcac agcgacagtt gagtcaaaag ccccttaccg gtttctggca
     4261 cggcaagacc acggtcattg cggcattctc cgtcgcggca attcttctcg atctagtgct
     4321 ccgcttcggc tttcacgcga cgccgcgaat ctaccagact ccgctgctgg ctgcgttaat
     4381 aatcggcggc cttccgcttc tctatgattt gcttcggaag ctgctgaagc gggaatttgg
     4441 ttccgatctg ctaggtggca tttccatcat cacttccatc ctgctgggcg aatatctagc
     4501 cggctcgatt attgtcttga tgctctctgg cggcgatgca ttggaaagtt atgcctcgcg
     4561 gagcgcctct tcggcgctgg tggctctggc gaaacggatg ccttcgattg cccaccgaag
     4621 acgtattgcg gaagtgcttg acgtagcact ggcagagatt gcggtgggtg ataccctcgt
     4681 gatttatcca cacgaaattt gcccggttga tggagtcgtg accgacggcc acggggtgat
     4741 ggatgaatct tatctcacgg gtgagccatt caagattacg aagactaacg gctcgacggt
     4801 gatctccgga gcgatcaatg gcgagtcggc cctgactatt cggacgaccc agcgtgcggc
     4861 cgattcgcgg tacgcgaaga tcatggcagt catgcgcgag tctgaggcta agcggccgca
     4921 gttgcggcgg cttggcgatc aactcggagc tgcttatacg ccggtcgcac tggccgttgc
     4981 gattctagct tgggtgatca gtggcgaatc gatccgtttt cttgccgtgt tggtcattgc
     5041 cacaccttgc cccttgcttc tcgccattcc cattacaatc gtcggctcga tttccctctg
     5101 cgcccgcaga gcgattattg tcaagagccc ggtcgtgctt gagcaaatcg ccaggtgtcg
     5161 gactgcgatt ttcgacaaga ccggaacgct cacctatggc gagccaagac tggcggagca
     5221 actcgtcacg cccaattttg cgcggcgcga ggtactgaca ctcgtggcaa gtctggaacg
     5281 ttactcgaag catccgttgg cgagggccat catagcgtcg gcgaaagagg aagccctaga
     5341 gctccctgag gcgaccgagg tgagcgaacc gcccggccag gggctacgag gaaccgtctc
     5401 cggccatcaa ttacaaatta ctagccgcgc caagctcgca ggacaaaaga tcgctggcct
     5461 tgatcaactc ccacaggtcg gcggcgggct cgaatgcgta gtggcaatcg acaatcgcta
     5521 cgctgcgagt ttgcgctttc gcgacgcgcc ccgagcggag agccgttcct tcgtgaagca
     5581 tttggggccc aagcaccaat tccagcgggt gatgatcttg tcgggagacc gcgagtcgga
     5641 ggctcgttac ctcgctgagc aagtgggaat caccgaaatc tacgcccaga agagtcctga
     5701 agataagctt gctatcgtcc gcaaggaaac tgctgtcgcg aagacccttt atgtggggga
     5761 cggcatcaat gacgccccgg caatgttggc tgcgacagtg ggcatggcaa tcggacagaa
     5821 cagcgacgtg acggcggaag cggccggcgt cgtgatcatg gataactccc tcgagaaagt
     5881 ggatgagttc atgcacatca gccgccggat gcgatcgatc gccctccaga gcgcagtggg
     5941 tggcatggcg ctaagcgtta ttggcatggc ttttgcagcg gccggtcttt taagtccggt
     6001 cagcggcgca atcagtcaag aggttatcga cattttggct gtgatgaacg cgttgcgggc
     6061 tgcctttgcc cccaaagtga ttcatgatct gtgacggagt ctcgaacatg gggatgcgac
     6121 ccttcagttt ccgcaacctc gctgaggcac aagggtgaag tcatatgtct gacgagattc
     6181 tcaaagaact tcagcgcgaa gcgcgtgtgc cgaaccggat tgtcgctgac caggcttttt
     6241 caagagccgc aggtgctcct ctgataccag gaaacagaat taggctcctc agagatgctg
     6301 cggagaatta tccggcttgg ctggatgcca ttcgtacagc cagaaacacc attcattttg
     6361 agagctacat tattcgttct gacgaagtcg ggtgccagtt tcgagatgcc cttgctgcga
     6421 aagcgcgcga gggagtgcgc gtcaggctca tctacgactg gatgggagca ctcggacaca
     6481 ctcattatcc tttttggcgt tcgctgaaaa gcgctggaat tgaagttcgt tgttttaatc
     6541 cccctcgctt ggacagccca ctcggatggc ttcgacgaga ccatagaaag atgctttcgg
     6601 tggatggccg catcggtttt gtgacaggcc tctgcgttgg tcgtagctgg gtgggcgact
     6661 caagacgagg cgttgagccg tggcgtgaca ccggtgtcga gatccaaggc ccggctgtag
     6721 cagacatcga gcacgctttt gcccagatgt ggatgatttt gggatcgccc ctccttacga
     6781 acgatctttg ggatgggaaa gacgctagac ccgagggtga tgtggccctg cgaattgtac
     6841 caagctatcc gaacatgggc ggtctctacc gcttcgatca actgatcggc gcgctggccc
     6901 ggcaaacgat ctggctcacg gacgcgtact ttatcgggac cgcatcatat atccagtccc
     6961 ttcgggcggc agcgttggac ggtgtggatg taagactatt attgcccaga atcaacgacg
     7021 ttccgatcgt tcggacattg tcgcgcgcag gatatcgaac actcctggaa gcgggagtgc
     7081 gaatttttga gtggaacggt tcgatgctcc acgccaagac ggccgtcgct gacggacggt
     7141 gggcccgagt aggttcatcg aatcttaacg tcgcgagttg gctcggaaat tgggaactcg
     7201 atgtcgtagt cgaacatgag ggattcgccc gcgagatgga acagatgtat ctgaacgatc
     7261 tggtttactc cacggagatc gtgctgagtc caaatcaaaa ggtacgttcg gctgggcaga
     7321 catcgcggca accacaccat ttgaaaataa ccgagggaag cggaggtcgc gcggcaaccg
     7381 ggctgcttag agtcggtaac accattggcg ccgcgatcac aagtcggcgc ggtctgggac
     7441 ccgcagagac gggcgttctg gcgatcgccg cagcgctact gctgtgtgtg gcgatcatcg
     7501 gtattctgtg gccacccact atttcgattc cggtcgtcgt attctgtgtc tgggtagtgc
     7561 ttgctttgtt cgcccgaatt tacagactgc gtatgaacca ggaccgatag cggcacgatc
     7621 aggagcgtcg ttcataattc ctgcatggcc ggtgtcggtt cttcatcgaa tccccgttgt
     7681 gcttgcaggc atccaagcat agggagatct ttgtgattaa ggctgactac acgaaccgca
     7741 ttcgggtagc tacgtacaat attcataagt gccgcggcct cgacggacgg gttcgcccgg
     7801 cccgaatatt ggaagtcttg cgcgaagtag atgcagacat tatcgcatta caggaagtcg
     7861 ttcgcattga ggcggaccgg cgcgaaaaag accaggcgaa cttcatggcc tccgaactgg
     7921 gaatgaattt ccactttggc gagaaccgcc aactcaacgg agggacatac ggcaacgccc
     7981 tattgagccg ctttccgttg cgcgccagct gcaattatga catcagcaca agtgaccggg
     8041 agccccgagg ctgccttcgt gttgacatga atctgcccgg aggcagtctg ctccatgtgt
     8101 tcaatatcca catggggaca gctttcctgg agcgcaggaa gcaagcgctg aaactcgtta
     8161 gtccgaaaat attacatcaa ccgacgctaa gtgggacccg catcgtgctt ggcgacttca
     8221 atgaatggac gcgtggactc accacccgtt tgctccgcca acacttcgtc gcgcctgatt
     8281 tgcgggatca catggagcat tcgcgcacct atccgggcgt gctgcctttc cttcatttgg
     8341 atcacgtata ttttgaccca actctccgtc tcgaccacct caccgttcac aaaactcgcg
     8401 cctcgctgat cgcatccgat catcttcccc tggtggcaga tttctcgctc actgcctgga
     8461 caaaagattc cacatccgcc aagacctccg acaaacgctc tgaggttgca gcctaagttc
     8521 cggacaagac cgaatcgttt acgagcccac cgtcatctgc tcgcaaaact ccagtttggt
     8581 tacgcagagt cgcgtcctgc agcattcaaa cccggattgt gagcactggg catgttgcat
     8641 gttgaatgat ctgatgtgtg atcgagcgcg gcctgtgcgt agccgctcgc gtaaacggcc
     8701 gtgcatcctt gacgccgagc acgacgaggt cgaagcggcc ttccctggac aattcaagaa
     8761 ttccgttcac aggcgagcag gactccacaa caacatcatc gatctggctt cggacctctt
     8821 cgggcacgag gttgatgagt ttccctcgca accgtttgcg agttttgtga cgctcgacaa
     8881 cggacttgct ctccttccca agaaaatgag caagtgcaag ttcagggcgc gaattcccct
     8941 taaataaaaa ttcgaagtgg ggaaagaccg attcagatcg cttctccaag ttagtggcaa
     9001 aaagtacatg tgaaatgcca tcttggcctc ggggagactt cgcgcgcggc ccgacggtaa
     9061 gtaccgggca atctacgctt cgaaacactt cttccgaaat agaaccctga agaaggcgat
     9121 tgagcccgat ggcctggctt ccgaggacga caagggagat gtcgtgcttt cctgcaaact
     9181 cgctaacggc gacggcagca tcgccaacaa tgagcttttg tgcgaacggc ttttgattca
     9241 gatggtgctt tcgaacgaag ccgtcgaatt gcctagacgc tacgcttcgt gcctgtgcag
     9301 tctcttctgc gctcacgctg aacggaaatt gattcaagac atagacaatg tcaacgctcg
     9361 aaccaaagtt gccgcagaac catcgaagcc agcctatcgc gcgggcggaa ttctcggaaa
     9421 gatcagtcgc aaacaggatg tttcccaact tcttgtgctc tttcataggt cattagctca
     9481 catctctcgg aatctcggta tgctcgctta gaattgaatc gtaaagatcc agagaaagca
     9541 tgttcttatc tttcaggctg aggcgggctt cccgttagtg tcgccaaact cctcacgcaa
     9601 catagatacc aaagatagta ttacagacat aactactggc ccaataaaaa ggccaacaaa
     9661 tccgaactct tggactccac caatgaggga aaacaaaagt agaagaggat ggagcttcac
     9721 tcgtgttgta atgaccaacg gacgaatgat gttgtcggcc gtccccactg caattgcccc
     9781 ccagatgagc aaaaatagtc ccttccacca gtgtcccttg acgaacagga agattaccag
     9841 cggcagccac accagagttg gtccaacaaa tgggatcagt gagcagacgg cggcagcgat
     9901 accgagcagc agggaggagc caactcccac tatcgcaacc cctgcgctgg tcaatatacc
     9961 ttgggccaaa gccacggcga gaatcccgta gaggttggcg aagacgcttt ctcggacctg
    10021 ggaaaatagc tgacgggtgc ggtcgcgatc cagtggcagc atggctacga ctcgattcaa
    10081 tatgtcagga ccgtcgcgga acataaagaa cagaatgagc aacgtaatga cggattcgcc
    10141 gacgaaaccg gccaaacctt tcaccaatga agtcgcgaaa gtgacgagca actgactggc
    10201 ttttatgggc agcgaatcaa ttgctccagc caaaccagat tttttgatat ccacatattt
    10261 ctcaagccag ttcattaacc tgtcaggacc ttgcaagaag taagggagga gccctccgcc
    10321 ttcggcggcc ctgtcattga acgatttggc cgcgtggata atctctccac ttgccgcaga
    10381 aaccagaaga acagccggaa ccacaaagat gagaaacacc gctagagtgg aaataagcgc
    10441 gtgggcattt gtttgagaga gagatctacc gatgcgtaca tgaagtggat agaagccgat
    10501 tccaatcacg agggcgaaaa cgatgggacg caagaaaggg cgcaagatcc cataggcaac
    10561 gatgattccg attgccaaca atatgagcag gacgactatg tttgcgtttc tgcgattcac
    10621 tcgttatacc cgcgtttttc tggctgctta gaccttcagg gcagtgcgat ggagcaaaag
    10681 tcccttggct cattttggac caaattgtac tcaaattaaa gccaactgtc cgctctggca
    10741 ctgggtctga cccagtaccg tccgctcttc atcattttca cagttctcgt caccatggcg
    10801 tctatgattg agtgccgctc ggcttgacgg gtgggcttcg atacgaatgc cgagagtcac
    10861 ttctagcccc aaatgagcga tttttaccaa cacgattcag ccgatgtttt aacccaactt
    10921 aacgtgaatg cgtctcgtgg gctcacggaa gcggaagccg ctcaccgcct caagaaatac
    10981 ggcaggaacg aacttgtggg aatgggcatc aggagtccct gggggattct gtggggccag
    11041 ttgaccgcat tgatggtcct aatcctaatc ggcgctgctg ttgtgtccgc actgatggcc
    11101 gattataaag acgcaatagc tatcgcggtc atcgttgtac tcaacaccct cctcggtttc
    11161 ggtcaagagt atcgcgccga gagggctatg gcggctctac aaaaactctc gctgccattt
    11221 gtaagagttc ggcgcgacgc cgaaattaag aatgtccaat cgcctcagtt ggttcccggg
    11281 gacattgtgt ttttcgaacc cggcaatata gtggccgcgg attgtcgcgt tttcgaaagc
    11341 acatatcttc aaacacagga atccgttctt acgggtgaat cgcaacctgt tcagaagatt
    11401 gcgagcgcct taaatcagcc gaacttacct ttgggggacc gccacaacat ggtgtacatg
    11461 ggcacctccg tgacggctgg ccggggacaa gcggtcgtta cggccaccgg tatgggtacg
    11521 gaactcgggc gcatcgccag ccttgttcag gccgtcaatc gcgaaccggt tccgctgcaa
    11581 cgacgtttgg ggcacttggg acgacgatta gccgtaattg ctctgctcct cgtagcggtg
    11641 atttttactc taggcttgct ccgtggagga gacttgaagc tcatgtttct cacggcggtg
    11701 agcatcggcg tggcggcaat accggagggg ctgccagcag tcgttaccat tgcgctgacg
    11761 ctgggtgctc aacgaatgct caagcgcaag gtgctgatcc ggaggcttgc ggccgtggag
    11821 gctttgggtt cggtgacggt tatctgctcc gacaaaacgg gaacgctcac cgaaaatcga
    11881 atgaccgttg cgactctcaa ccttgctaat cgtgttctcg aactaccggg caatcttcgt
    11941 aacagagctg tagaaaggcc acccgtcgaa gagatgcggg attctggttt caagcttctg
    12001 ctgggaggtg ccgcgctttg caacgatgcc atcgaacagc gaaatgatca gcaacctgga
    12061 tcagcagtca ttctgggtga cccaactgaa acggcgctgc tgactgcggc tcggaatctt
    12121 gacctctcaa agaccgatct agagcgattg atgccgcgtg ttgaagagat cccctttact
    12181 tctgagcgaa aacggatgac gacgatccat ccgctgccca ccggtccgcc cccgattcct
    12241 attgaaattg caactccgaa caactcgggt ggcctgtcct atgttgcttt caccaagggg
    12301 gcaccagccg agttgctgcg tgtttccgac acagtgtgga tggatggaaa gatagaagat
    12361 ctgagcgaat cactccgcca gcatctcgga caagccaccg atgatcttgc tggaaaagga
    12421 atgcgtgttc tgggcatcgc gttccgctac atgggctccc taccagcctc gatggatgat
    12481 gaagcaatcg agcgagaatt gacgtttgtg ggcctcgtgg gaatgatcga ccctcccaga
    12541 tcggaggtgg tggctgcagt tgcgacctgc ggcgcagcgg gaattcgtcc ggtgatgatc
    12601 actggggacc acgtccttac agccaaatat atagcagacc aggtcggcat cacggacgaa
    12661 ggggagagta tcgccggtcc cgaactcgac cacctttcca acgaccaact aaagagtgtg
    12721 gccggaagga caagcgttta cgcaagagtt tcgccggaac acaaactgag gatcattcag
    12781 gctcttcaag aacgtggaga ggtagtagcg atgactggcg acggagtcaa cgatgcaccc
    12841 gcactcaaaa gggctgacat tggtgtggcg atgggagtca caggaagcga tgtcgctaaa
    12901 gaaacggcgg atatggtctt gctggacgat aacttcacca cgattgtggc cgcagtggaa
    12961 gagggccgag tcatttacga caatgtgcgt aagttcgtaa ggtatatctt ggcgactaat
    13021 tccggcgaga ttttggtcat gcttgttgcg ccattccttg gaatgcctct gccgttgcta
    13081 ccgcttcaaa tcctctggat gaatctagtc acggatggcc ttcctgctct ggcactcgcg
    13141 atggaaccag cggaatccga cattatgcgc cgtccgccgt tcgccccgca ggagagtatc
    13201 ttcgctcgcg gcttgggtag ccacattgtt tgggtaggca tactgatggc gtcactatcc
    13261 acgggggtgg gctatttcta ctggcgagca gaccgtccga actggcaaac gatggtgttt
    13321 accaccctta cgatctcaca gatggcccac gtgatggcga tccggtccga acgcaattcg
    13381 ctgtttcgga tcggattcct gtcgaacaaa cctttacttg gagctgtgat gctgaccatc
    13441 atcctgcaac tcagtttgat atacgctccc gttctacaag gggtcttcag gaccagtgcg
    13501 ttgtcacctc tcgatctcgg attgagcatt ctgctcagtg gttgggtctt tgctgcggtc
    13561 gaatttgaaa agtggctgtt ccgagtccag cgaccagatc ctcggtaacc gacgctcctg
    13621 cgttcgttcg agcgtcatat cggtctcacg ctaagccgag atgcagcgga tacgttacgc
    13681 agtgcttccc cggcagcagg ccgtcacaaa cgtcactgcc tgtaaagtgg ccgcagctag
    13741 cacctacggg ccctcgttca ttccccacca gaatctgagt tgtctatttg atgacaaata
    13801 cagatacgtt cactaatgcg tgtatctcca taaacgtcaa aagcgacatt cctcacaaca
    13861 gtctgtgact tgcatcacat acacgcccca gaccccaaga taaaaaggcg acagcgttaa
    13921 ggagtatctg acacagtcag ggagaagaga ggagaaaaca atgagcaaaa tgcgaatagg
    13981 tattcggggg acgaccgccg tgtcaattct ggccctactt tcggtgctat ttctttccgc
    14041 cgcaccggcg gtacttgcta gcccgagacc gaaagattcg gagcaggtta caaagctttt
    14101 gacgaacgtc aagtcagagg ccgttcaact gaaagacgat gccgaagaca tgaagagttt
    14161 tacgtggtcg aaacaactaa gctggcagag tcacgcggcc aagatcgaac agatcaagca
    14221 gcacgtcaac gcgtccggca aattgcttac cgacctccag aattcgaaag cgtcggcttc
    14281 ggcgtggcaa ggacaagcga ttgaccgaat tgctcccatc ttgcaagaac tggcagcaag
    14341 tgtgtcttca acgatcgagc acttgaacaa gaatcaagat cgaatccata cggcacccta
    14401 tacggattac gtcgcgtcaa ccgccgatct ggcctctgac ctgtctgagt taattaccga
    14461 ttacgtggca tacggcgaag ccaaaaatag atctgaaaac ctggcgcgga aactcgaagt
    14521 gcccggcgcg taatgcgcag cggcgctggc tgggcgggtc tcgcgcactc agccagcaag
    14581 ccgcatcaag cggagggtgg gaactgaatc gcacgcgtaa tgaatgggaa gcgcaagttg
    14641 aacgaaaagg cactacagcg acagggaggc caagtacaac agcttatcca gcatcgggat
    14701 gattttccgc tcgggatcta tcgagaactc caaagcgtcg attgcgaggt actgctcgtt
    14761 attgcccctc actacgcggt atcccgtact atccatgttt acgtagatcg tcgttagaac
    14821 gtcaccttcc ggtccgtatt atgagacggc cccgcttctt ttccgagcag atcccacgtg
    14881 ccttcagcga gatagtgggg ccttccttcg ccatgctggg ggaccatgcg aatctcactg
    14941 atatgttttt ggagttcggt cctagctggg gctgtttcgc cactcaaaag cccttgaaga
    15001 tcggctagcc tcttcgtcac aaactgccga atagcactca ggtgacgcga tcaagctgcg
    15061 agcggaactc acgggcacgg gtacgagctt cgaggagata ttcctcatca agatcaccga
    15121 tcctgagaag aagcaggaga agaagaaaga gggaaaggat gagccggata acacgctagg
    15181 cctcccaaag ctcgcgctcg tttacaagga cgaaggccag ggcgaaatca cctgggcgca
    15241 gctggaggag cacaccagcg ggtgcattac aacaccgcga tcaatctcga taccctatcc
    15301 ctcgcgatct gcgatccagc cttttcgaaa gagatgaaca tcaccctggc cgcatacgga
    15361 cggtcatttg gctgttgctg actgtgcacc aacggcggaa acgatccgta tgtcattttg
    15421 aacgttggat acgcctggtg cggcccaagc cgcccgctcg gcttcctctc gttcagccca
    15481 agttcggacg tttccccgca ggatgacggt accggcactc gcttccacgg caatcttctc
    15541 cgcgtctacc tgtgcggctc gatgcagcgc cttcttaatc gcgtgtatga catcttccgc
    15601 agaaacgcgt ggctttataa cgatcacgtt ggacacgccc ttcacaccct ttaagttgcg
    15661 tattgcgcct tctgccgcgg ccttctggaa atgatattcc acgcttccgt cgagagtgac
    15721 ccatccgttt tcaacaagga ctttgactcg tttgggcggt acggaagcat tccagtccag
    15781 cgcattgact gcggcttggg cgatgtcctg gtcgttgtgc ttgccatctc cagcgagttt
    15841 aacggtgagt tcgtctgtaa cagcccgaac tccctccacg cgttgagtca cgcgctccgc
    15901 cgtccacttt tcggtgagac ttttcacggt gccattgagt atcacgacgc cgttctcgac
    15961 ggacacgcca atttcggcag catcaacact gggttcaaaa acgagctcgt ccagaacatc
    16021 ttgctgcaat tgcttatctg tcttcattgt catctcctct tcattttcga actcggaaat
    16081 tccacactat gccctcaacg caactacgcg ggcgtcactt cgacatgcgt gccttttgtt
    16141 ttggggttct ttggcatttg aagttcgagc atgccattct tcagtgtggc tgtggctttc
    16201 gacgcaacca cctcggctgg caaatcgatg acgcgtaaga tctcgttcga gcactgctct
    16261 tggtacacag ccttcccctt cttttgctct tctttggttt ccttcttgcc gctaatcgtc
    16321 agccgcagag gttcgatgtt gacctgcaga tctttggctt cgaagccagg aacttccgcc
    16381 tcaaccgtga gtgcctcatc cgattctgcg attcggacgt gaaccgggtg aagcaattcg
    16441 gattcggcct tgaaccagtc gtccaactca tggccaaaac ttctgccgtc gctttcgaag
    16501 atttcgaacg cgcgtcgggc gattaagttg tagatctgtt cgaatcggtc gaacacgtcc
    16561 cgcggtccca gtgcttttgt tggaacacgt gccatctgtg ttgctgtagc attcgctgtc
    16621 atctcatttc cccttttcaa aaattggagc cgatccatac gacctttgaa agccttctcc
    16681 accggctacc tcgaatgaac gatacacggc cttatggttg aacgatgtga ttaacatcac
    16741 agattcgagt ggcgacaatc acatcactat ctcagctgtc tcatatcatg agcatgaggg
    16801 ttgcaaacat ctgtcaccct aacgaacgga gcagccacaa tgcctttcaa acgtcttctt
    16861 ttcaaatccg cagcccgaga gaaagtccta aaaggtgcga ccgcgctgac agaggccgtt
    16921 cgagtcacgc ttggcccgaa atcgaagtgc atcctcattg agaagaagtg gggccgcccc
    16981 atcgtttgca acgacggcgt cacaatcgcg aaggaaattg aattcgaaga cccggaagag
    17041 aatctcggtg ccaagatgat tcgcgaagcg gcagagcgca ccggagacgc agtgggtgat
    17101 ggcacgagca cttccgccat actggcgcat gcgatttttg ccgagggact gcacaacgtg
    17161 gcggcgggcg caagcgcagt cgaattaaag cgcgggctgg accgaggcct ccgggctgcc
    17221 gtcgaatcgt tacacggtat ttcccgccca gtttcgagcc gcctggagaa ggcgcagatt
    17281 gcaaccgtgt ctgcgcacaa cgagtcgacg atcggcgaat tggttgccga cgcgatggag
    17341 aaagtcggaa aggatggggt ggtcaccatc gaggaggcaa agacaacaga aacagcgcta
    17401 gaggtagtcg aaggcatgca atgtgatcgc ggctatcttt ctccttattt tgtgaccgat
    17461 cctgaaaaga tggaggtgat actcgaagat ccagtcatcc tcatccatga gaaaaagatc
    17521 accaatctcc aggagttcct tccattcctt gagaaggttg ctaaatctgg ccgccccttg
    17581 ctggtcattg ccgaagatgt tgaaggagag gctcttgcga cgctggttgt caacaaggtt
    17641 cggggcgttc ttttttctgc cgcggtgaaa gcgccgggat tcggtgaccg gcgcaaggag
    17701 atccttcagg acctagccgt tgtgacggga ggcaaagtgc tagcagaaga gctgggcgtg
    17761 aagatcgaaa acctcgagct cgacgagctc ggcaaagcca agcgagtcat catcgatcgg
    17821 gaaaaaacga cgattgtcca aggcgccggg ctgaagaagg ccattcaggg ccgctgcaac
    17881 gagattcgta agcaaattga ccagacgact tccgattatg acaaggagaa attgcaggag
    17941 cggctagcca agctttcggg aggggtcgct gtcatccgcg tcggtgctcc ctccgaaacc
    18001 gaaatgaaaa atcgcaagga agcattcgag gatgccatca gcgcgaccaa agccgcaatg
    18061 gcggaaggaa tcgtcccggg cggaggactc gtcctactgc gcgcaatcga tgccgtccag
    18121 cgtgcggaga gcgaatgctc tggagacgaa cgaaccggac tggtcattct caagcgagct
    18181 ctcgaagctc ccacacggca gattgcagaa aactcgactc tcgatggcgg cgtggtggtg
    18241 gaccgaatgc gcaacggagc tgggaatttc ggacttgacg ctgcaaaggg agaatatgtg
    18301 gacctggttg cggtaggcat tatcgatcca accaaggtcg tccgaacggc gctcgagaat
    18361 gctgtctcgg tggcaggcgt gctcttattg actgaggcaa ctctcaccga agtgcctgaa
    18421 ccgaagatgg gtcggacacc ctctcacgac atgcccctgg aatgaagtct ggcttctacc
    18481 agctgctggg cagccaaaac aggagccgaa aacaacgaat acaagtttaa ggggatgatc
    18541 ggagaccagc tatggcgaac cggctgacgg gaaaggtggc tttgattaca ggcgcggccc
    18601 gcggaatggg cgaagccgac gcacgcattt tcgcgcaaga aggggcaaga gtcatagtcg
    18661 cggacgtcct ggacactgag ggccaacgag ttgccggtga gatcaaccat gcacatccca
    18721 gctgtgccga gtatgttcat ctggacgtaa cgctggcatc ggattgggag aatgccgttg
    18781 cgacggcgga acgatccttt gggggactcc acatcttagt caacaatgca ggcattctca
    18841 gttttgctgg ggttgaacaa accactgaag ctgagtggca gcgcattgta gacgtaaatc
    18901 agaagggcgt ctggcttggg atgaaggctg ctgcgccagc cctgcgccgt gccgggggcg
    18961 gctcgatggt caacatttcc tcgatttacg gcctgatcgg ttctggaggg gccgccgcct
    19021 atcatggcac caagggagcc attcgaattc tctcgaagac cgccgccgca gaattcgctc
    19081 ccgcgaatat tcgcgtgaat tctgtccatc ctggaatcat cactacacca atggttgagc
    19141 cacttgctca ggacctgcga aaaaacatcg cttctgccgg gtgcattaaa cgggaagggc
    19201 gacccgagga agtcgcctat ggcgtcctct tcctcgcctc agacgaagca tcctatgtta
    19261 cgggggccga actggttatc gacggcgggt acaccgcttt gtagtttttg cacccccaac
    19321 gtgcgatttt gtctccgcac gccggaagtc ctcgggctgg taaatcctgg gtgcttttga
    19381 cgattggtga ttgcggtcac agaccttctg cgatgaaggg tctcctatga aactgcgcat
    19441 tgattgtgag gttgtgctcg atcttagaaa gccgtagcca agcctaaaaa gtgtcccgaa
    19501 atagtcaccg ctgatgtgcc aatcggcgtg cttgagatgt tgccgctatg gcaactcgat
    19561 gtcgtcgaac cggacacgac ttggaagccc tgggaccaga agaaggttaa ccggcttctc
    19621 gaaacaggat ggcgtttgct ccacatctac acacggaagt atgaggaaga cggcgtgtga
    19681 cgcgaacggc ccaatgggga ctccggggag attaaaaagc ggccagcaac cagaccaaag
    19741 aaaaagtgga ccgccaatga ggcaatcatc cgaactataa cgcatgccgg aggcatcgcc
    19801 atgaaatcac gactgcagat tacatttcga aatatggagc cgtcgaagga aatcgaagaa
    19861 tggatccgtg ccgaagcagc caagctcgat ctcctctaca atcaagtgat gggatgtcgc
    19921 gtgatggttg aaattcctca tcgtcaccac aggaacccgt acagattcac ctgttcgttg
    19981 agcgcgccag tttcggcgtg gggcgaccca cctgccgctt gagcgaaggc agctgtcgag
    20041 agaaagcctt gatgcaattc tcgttgaacg tcctcatcca actggagtgt tggcaaggga
    20101 agcgcttcgt gtctgaagtt ctcgaaattc gcggccacga tcccgaagct gatcattacg
    20161 acctttatcc gatttttgcg gcgcagtcct gatggcggag tctattcaga cttatccttc
    20221 attgccttta gacttgcttt caccagtttc ttcaccaatg tcattggtac cggtttaccc
    20281 agggggaagc taacaatggc tttcgtcgta gcataacctg ctagctcctt cttatgttcc
    20341 tgtattactg gagggcgcac atgtaggcgt atgtgtggtt tcttgaagct gaaccaagca
    20401 aacatcccgt catagtcaaa tccaggatag gaatatccgg gaatttggaa ataatcggtg
    20461 cgttcggcgg caccgggtgc tgccgcccgt atggccgctc taatcttcgc cagatcgcct
    20521 tgcgcctctt ttgggcacgt tgctatgtat ccgtctactt cactgctttt gcgctttctc
    20581 acggctgcgt ctccccttgt cgttctgatt ttttcatcgt aatcgacagt tgtgatctca
    20641 tcgacccggc atgcagatta actcacgctt ttggccatag cgaatagctt taccaccccc
    20701 atttcctctt ggtttcagga ggacccatga ccctcgcatc aaaatccgtg tgcgacgaag
    20761 agaccgtcaa caacgctgga aagttggagg cgccgagcaa tcagatcctc cttgcttgca
    20821 ttctgctgct accgaaagat gccgcgccac ttttggccct cctcaagcaa gtgaatggaa
    20881 attccgggcc atctaccacc gggcaaatcg ctgacgttcg cacgatgaac tcgccacggc
    20941 ctcaatctga atcgtggctg gcacatctag aagcagccga ctacctcggt atatccaaga
    21001 gcacgctcta ccgcttcgcg tgcgagggga gcattggaag aagtggcacg agttgccgcg
    21061 gaatgattca gccgtcattt caaatccgtg caaaagcccc aagttcaatc ggcccgagcc
    21121 gccaagggtc ttggtttggc ctgaggctct gctccctctg ctgattcaac tcggggcgcg
    21181 tcagcgtttg ccgctcgcgc tggcactctt cggttgtcga agccaagcgc ctattccctc
    21241 cactttgttc aagcgctcgg cggcatcacc gctcactacg tcaatctcaa cctggtgtct
    21301 tcgtgccagt tcgggcaaag cgtcccggag agtgactttc cgccgcttgc tcccgcaaat
    21361 agagcatgtt ggatcagccg accggtccat ccagccacac tgaacgcatt cacgcaggtc
    21421 cgcatcgagt cctcgggcaa gcaccagggt acgtatcttt cccttctgca gatgggcaag
    21481 tatttcatcg aagccggcga tagtgccgcg ttcctcgtca gctacggcgg cgacaatctc
    21541 tgcctcatgc gtgcgctccc agctcgcaat ctggggttcg agatgcttct ggaactcaag
    21601 cagcgttatc cgagccaggt cttggtggac caagacaatc ggttgtcgaa agcctcgtgg
    21661 aaacgctgcc accatgggcc ttattagtcg atcaagaccg accagaaaga cggccgcgag
    21721 atctttcttt ttgcaaaaga tgaccgcttc ctgggccgtt tcatggcaca agtgtgcgta
    21781 ctgactctcc atgcgcttct tgaagatgtc tctctgggcc ccccggcctc ctgctggaat
    21841 agggcgtaat cggcctttga agccgctgtc gacgacgtgg ctgagttctt tgttcttcca
    21901 ctgagaggtg tctatggcga acttcctctc ctcatgctca accatctcac ccagccagta
    21961 atggaaaaaa cgtgcacccg catggtccac cgctacgatg ccataaggtt tgtgctcact
    22021 tgcatgccag agcaactgcg ttagcgccgg ctttccccag tgaagttcgt tttcgacttc
    22081 tcgttgcaga gatgcaatct cccaaacggc aggccctgca aaaatcacta ggcttctctc
    22141 atgcggttgg cgctgtcgca agaactcttc gacacggtcc agttgcttcc ggaacagctc
    22201 ttgctctgtg ggaggaagac tctcggcaac gggtcggctt tcgtccttca gccaagccag
    22261 gtattcgggt gtagctccgt gcaaaaaagc ctcaacagac acggttcgca cgtaagccgt
    22321 tagcaaagga gggggtagat tggagaacga ctctaattga ctcatcgtaa ccatattgtg
    22381 tctccagact tcgcacgttt ccggtatcgc catacgcatg tttccactac attcacagac
    22441 tgctctaatc gatgaacaat cggcgtgatc tgcatcacat cgagctaggt gtagacgagc
    22501 taaacttaca cccttctcgc aaactcgata agcgcttgga cttgacgggg aggcattcgg
    22561 aatcccagta caggagttgt tatgctaatc caagaaatga cgaaacaaga gagtctggac
    22621 ctgttggctc gtacgcgtct ttgtagatta ggctgtgtcc aaggaaatca gccctacgta
    22681 gtgccattct atttcgccta ccagaataat tcactttatg gcgctagcac ggtcgggcaa
    22741 aaaatcgact ggatgcgcaa aaaccccctg gtctgcgtgg aagtggacga ggtggaaagc
    22801 ccgcagaaat ggatcagtgc agtcgttttc ggtcgctatg aagagctgcc cgatacgccc
    22861 gaataccacc gcgagcgcgc agtcgctcac aatgtgctgc aaaagcagcg tatgtggtgg
    22921 gagcccggtt acgtgaaaac gatcctgcat ggcacggagc gtccgctcgt gccggtcttt
    22981 taccgcatta gcatcggtca cattaccggt caccgcgcga ctccctgagc tcggcgggct
    23041 cgattggctc aaagctgtca caccgaatag tgataccggt cacagctata tagctaaatc
    23101 tgggtccatt ctataagtag gcgcagtacg ccatgagagt tttccccatc aattgcccgt
    23161 tgacgcgatt tgagagcttt cgcacaaaga tgctcgcaaa ggatctgatg accagtgcgg
    23221 tcatctgttg cacgccatgg gacaccgcac agaaagccgc aagtttgatg aaggcgtacg
    23281 gggtgggcgc tattgcagtg gtggctggca cattagatcc cctgcttgaa gggatagtga
    23341 cagatcgcga cttgtgctgc ggtgtgatcg ctgccgggaa gaaccttgat gcggtccagg
    23401 ttgcgaatat catgacacga attcctgtga cgtgtggccc ggaacataca ttggacgagt
    23461 gcctggagct gatgcaggaa aaccaagtcc gcagaattcc cgttgtaaac cctcggggaa
    23521 ggtgcattgg cattgtgtgt caggcagata tcgcacttca cgctcctgcc gcgcaagtcg
    23581 caaagacaat caaggaattt tctcaacctg cgaaaccctg cctggagatg tatttcgaga
    23641 agggttactt ctattgcgga cagcctcacg agcaggacca aatcttgctg ctcaatcgcc
    23701 ggcgagaatt gaagcacaag cacgaggtgt acgtatgatc tttgcaaatc ggacagaagc
    23761 tggtcaggaa ttggcgttgc gtttgagaac atatgcaaac cgtgatgatg tgattgtatt
    23821 gggagcgccg cgcggaggag ttccagttgc tttcgaggta gccacggccc ttaaggcgcc
    23881 actggatgtt tttgtgttgc gcaagctcgg cgttccgggc cgcgaggagt tggcattcgg
    23941 agccattgcg agcggagggg ttcggattct tgaccgggat acgattgaga gcttgggaat
    24001 aactgggttg gatatcgagc gagtgacaag ggtagaaaaa caggaactgg aacgtcgcga
    24061 acagtcttat cggatgggga gacccgcgcc agatgtaagt ggactgaccg tcattctcgt
    24121 tgatgacggt attgcgaccg ggtctagtat gcgcgctgcc atccgtgcgc tacggcagat
    24181 gaagcctgcg cggatagtcc ttgcggtacc tgtggccccg ccttccacct gcagtcaact
    24241 ccaattcgag gttgatgaac tcgtgtattt ggagatgccg gagccgtttt acggcgtcgg
    24301 acgattctat gatgacttta cgcaggtatc agacgaggac gtaaaagagt tgctcgatga
    24361 agcttcgcgg cagcgccgtg caccgcacta tcagggtgtg cggaccataa cgccaggaac
    24421 acagcaatga gtaccagccg agtgctcctt acgtgatgct actgaaactg ccagtccaaa
    24481 tcggtagcgc gtttgcgaga agcgaggtgt cagaatggtt cctaaacacg aacacgatct
    24541 tatcaaagcg atccgccaag cggcccatcc gctgacggga gaaacggcgg attttgatcc
    24601 tttgcttaaa atgattggtg actctcggtt cgttctggtc ggagaagctt cgcatggaac
    24661 ccacgagttc tatcgcctcc gcgcgcagct caccaagcgt ttaattgcgg aaaaggggtt
    24721 taccgcagtc gctgtcgaag cagactggcc ggacgcatat cgtattaatc aatttgttcg
    24781 gtttgatggt gaagacgaag acgcagtcga cgcccttgca ggcttccgaa gattccctgc
    24841 atggatgtgg cgcaatgctg acgtcctcga ttttgtgggg tggctgcgca attacaatga
    24901 gcaccgacat tcccagcggg ttggtttcta tggactggat ctctacagct tgcatgcctc
    24961 gatgcgggca gtactggatt tcttggacaa agttgaccca gaggcggcac gccgcgcccg
    25021 atatcgctat gcctgctttg agaactttgg ggaagacact caagcttatg gttatgccgc
    25081 cagcttcggc ctgagcaaga gctgtgagaa tgaagtcgtc agccagtggg tggaaatgcg
    25141 acagcgggct gcggatctgg ctaggcgcga tggccacgtc gccccggatg cattcttctt
    25201 tgccgagcag aatgctcggt tggtgaagaa cgccgaagaa tattatcgct cgatgtttca
    25261 cgaacgaacc tcatcctgga atctgcggga cagtcatatg gccgagattt tggacgcatt
    25321 gacgcaccac ctgggtgcga agtcgaagat cgtcgtctgg gcacataatt ctcatctcgg
    25381 agacgctcgt gccaccgaga tgggacaaaa gggcgaactg aatctcggac aactcgtgcg
    25441 agagcgatat gacaaagagg cgatcctggt gggcttcaca acctatactg gcacggtcac
    25501 tgcggcatct ggatgggata gcgctgccga gcgaaaacat gttcgaccgg cgctgccggg
    25561 cagttacgag gcacttttcc acgaaaccga catacagaat tttctgctta ccttggaccg
    25621 gaaggaaaaa gtcgcgtccc ttttgcggga accacgcctg gagcgagcaa ttggcgtcat
    25681 ctatcttccg cgatccgagc gtgccagcca ttacttccat gcccagcttc cggaccaatt
    25741 cgacgccatt ctgcatttcg atgagacgcg ggcggttgag cctctcgaga gaaccgccgc
    25801 ttgggagact ggtgaggttc ccgagactta tccttcaatg ctgtaaccaa gaccaggaag
    25861 catgcggacc gggagccggc cggacacgcc aaatcgtttt cagcagcttt tgacgccccg
    25921 ccgaccgtat agcgccttca gaatggaatg aggctaacga tcctcgccgc aggcatggga
    25981 atcatttgtg tgtttagaca caagtgtatt gacgacacct ctggggcagc gaaagcagct
    26041 atagaagcat cgctagaatt ttgctggcat agaccaattg ccgataaccg ctccagtcag
    26101 attaagccgc ggcagccttc tgccgcaccg ctcgaagagg tcgcggcgcg taaacgattt
    26161 tcagcgtatt tgcaagttcc gcgagcgaca attctgagtc accttgtggc gtttctgttt
    26221 cagtacgaac gacgatgtcg tcgatcgaaa gtattccttg aagcttgcct gcagcatcga
    26281 ttacgggcaa gcgccgtact ctcgcctggg ccattatcgc caatgcagca cggaggtcat
    26341 catccggttt gcatgcgaca acctttccag acgttacttc gctcacagtc agttcgctcg
    26401 gcaaccggtt ccgtgtaccc aacgcgacgc agatgtctcg gtctgtgacc agaccaacga
    26461 cctcttcctt ggaatccaca ataggaagaa tcccacagtt ttgtcgccac aggatttcca
    26521 cagcagatcc caagttcgtc tgcggactgc aataagctgg cggcttcgcc atcacatctg
    26581 taactttcat agcttttctc cttttccgat tgaggttttg ttttcgcaat gtgtgcgtgt
    26641 ttcatagcca ttgactagcg ggtctttccc gaaggcagcg gaccgcgcag ccagcgccat
    26701 cggaaacaca atcgcaaact ataagaacta gcacagtgct atggatcacg cttgtgtgcc
    26761 cccggagtta cgaaggaatg gaaagtgggg acgagagatg ggcgtccagt tttggcgggg
    26821 tgcactgccc agatctccgt ccccagagat caaaacgatt caggaacggc taggccatgc
    26881 ctctgccggt tccttgacgc tcgatgtgta cacgcattcg gagtggagtg agaaggtcga
    26941 agcggcgcaa gtttcaggcg agcagatcga aaaagccgtg aattctgtca gcttaactgc
    27001 aatcgaagaa aaagggcctg ccgaccgaaa ccagcaagcc cttcagtacg cgtaagatag
    27061 ctggttgcgg gggcctgcaa ccatccgaat tggttggtac tgcccttcca ttttgtattg
    27121 agaaaaacag ctgcctagtc ggacgggaca tccctcctgc ctttgtggtg aagaggaatt
    27181 cccgcgactt tgtctcgaag ccgttcaatt tcctcgtgca aaaccctttg caattcctga
    27241 gcgagcttta gcgacaaacg tcgtcttcgg actttgcgag ccgctcacac agatctcgta
    27301 cacgagcttc gaatggcatt ttcgtgagcg caaaaagcgg agtatttcat tccaacggga
    27361 cgataaggaa tacagtgtaa cctgtaccgt tgcaatgctt ggcccgaggc cgctgcctca
    27421 tgctggttgt gcacacggca gcgcgtcgct ctgcggccat gccgagcatc cctttgaaaa
    27481 aaaccgagat ggatcattac gcacaggcag ttacatatat tcgtagttgc gaatatacta
    27541 gagtatatga gagacgcact gcgccagttc aagagtgaga tttttcaagg ccttgcccat
    27601 cccacccgga ttgcgattgt tgagcttctg cgcgagggtg agttatcggc cggaagtctg
    27661 attgaaaaac tccgcatcga acaggccaac gcctcgcaac atctggcggt tctccgcgcc
    27721 aaacagatcg tggtcagccg aaaagtcggc aatcaagtgt actattcgat ccgcgatcgt
    27781 gcgctgattg aggtcctcga tatcctccgc cgctattttt acgctcagct ggacaacacc
    27841 gtaaacatgt taagggaagt cgccagcgag caaccaaaat cacgtctcag gacgaatcga
    27901 tgaccgattg cagccctccc aggctggctc ggctggacag ccttcgtcgc gcagttgagc
    27961 aggcagcgca cctgctcccg atgcagggcc cgattggcgt ctttgttcat cacaacacgc
    28021 tgcatgcttt tgaacacctg cccttcgaag aggctgtaat acaggcggca caactgttcg
    28081 acgctgagcc ctacatgagc gaggcggcct atcgggccga gttgtctcgg gggcgtattc
    28141 aattcgtaga tgttgatgcc gtgctcgacc gcgaaccgga ttcgctcatc ttcgcgcgcc
    28201 taagtcggag ctcgctgcgc agagccatga ttacacctgg ggtcagggaa ttcgacggcg
    28261 caacgattat ctggcgtacc gaacagggcg atctggctaa ggactttcgc cggccagcat
    28321 tgcgggcact gttcgatgcc tgctttgcgc gcgccgtggc tcatgaagaa gaaccaactc
    28381 cgccccggcc tgtggatgag gtgatccatc cgtggctgat ccggctctgc tctgtgttct
    28441 tggatcaagg cacggcgtat tggtccatgc caaaccgcga gaaaggcttt tatgaatgcg
    28501 tcagggcatt gttcttgtgc cgtggtggaa tgttccctag gtatcttgtt ggactagacg
    28561 acgaatttgg aaagcaggag tgcctttcct tttcagcatc ggacgcggtc ctggattatc
    28621 tggacagtca gtctttccga gaagccgaat ggcctgaaat cgttaaagcg gaacttctgg
    28681 cgctacccgg ttgggcgggc ttgatgcaca ggcttgagaa agatccctct ctggcgccgc
    28741 acaaaaccct cccatgttcc ttgatggact tactcgctgt gaggctgacg atgtcccgcg
    28801 tagcccagcg caggggagca gcggagactg tttcacaaga gagtccgctc aagacacaag
    28861 agaagaggcg tctgagccgg tcggcgcgta tctatgatgc cgctcgcgtg ttgaggcttt
    28921 ccgctggcga aatcaatcgc ctatcggaat ctgagtggtc ggcgttggtc aatgaagtta
    28981 aagcgttcaa tggtttggag cgcaggcgga ttctacacct cgcttatgaa cggtggcacg
    29041 aacgcgagat tctacgcggg ctagcgagcc acagaaaata ccgcggcctc ttcgatcagc
    29101 gggggagacc cccggcgcaa gtcttcttct gcctcgacga gagggaggag tcgatgcggc
    29161 gtgctctcga agaagctgac cctagggttg agacttacag cgccgccggc tactttggcg
    29221 tggccgtgga ttacaaggga attgacgatc cgcacggcgc ggcgctttgc cccgtggtaa
    29281 tcaaacctga gcacgccgtc gtggaacggc cgaaagcgga ggacagcgtg ctgctagaga
    29341 gtcgccgttg gcgtaggcgc attcttggat tgctgatgcg gaattctttt gtttcttcaa
    29401 agacattgct gcggggctgg ctttccactt cggctctcgg cttgctgtcg gcagttcccc
    29461 taattggtca tctcttggct cctcgacgtt atgctcttct tcgtgactgg ctaaacaggg
    29521 agtttctccc agagccgagg actgagctta cgttgatgcg aaacacgaag gaatcccaaa
    29581 acgtggtgac aggtttattg acggggttcg ctccgggcga aaaggctgaa cgcgtcgcca
    29641 gcgtgttgcg cccggctggc cttgcgcgcg gatttgctcg tctagtggtg atcctgggtc
    29701 acggatcgac gagcctcaac aatcctcacg agtcagccca cgactgcgga gcctgtggag
    29761 ggcgtcgcgg cggtccaaac gctcgacttt ttgcagcaat ggccaaccgg ccggaggttc
    29821 gcgagcgcct gcgcgaggaa ggcatcgaca ttcccgggga cacatggttc gtcggcggct
    29881 atcacgatac ttgtagcgac gacgtggaac tcttcgatct cgatgcggtt ccggccaccc
    29941 atcagtcaga cttggcctcg attcgcaagt ccttggatca agccagagcg caaaacgcac
    30001 aagaacgcgc ccgacgattc gaatcatctc actcagaagc ggatccacgc cggtcgcttc
    30061 gccatgtgga ggagcgcagc gaacatctcg cacaaccgcg ccccgaatat gggcattgca
    30121 cgaattcggt ctgcatcatc gggcgacgca gcttgacgcg cggcctattt ctcgatcgtc
    30181 gggcctttct tgtttcctac gatgccagcc aagatcgcga cgatcacagc ctctcggcgc
    30241 tactgtccgc agccgtgccg gtctgcgcgg ggatcagtct tgaatactac ttttcgttcg
    30301 ttgacaacga caagtacgga tgcgggacga agcttccgca caacgtgacg ggcttggtgg
    30361 gggtaatgga cggccacgct agtgatctcc gtactgggtt gccatggcaa atggttgaaa
    30421 tccacgagcc tgtgcgaatt ctttttgtga tcgagacgac cccaaacagg ctcgataagg
    30481 tgatcaatgc gagcgcttcg ctggagcagt tcgtcgaaaa tcgttggatt cgactcgcga
    30541 cgatcgatcc gatctcagga cgcgtccacg tccagaggga cagcggatat gaggagttcc
    30601 atggagcgtt agaacggctg ccggtagcgc tctcgtcacc tgaatggttc gctggcaagc
    30661 tccagcatct tccgatggct tggattcaag cgcctcaaac gccggccgga gacagcatga
    30721 gatgagtctc tcgcaagcca ttctggtttt cgtggtgctg gctccgggtg tcgtgtttgc
    30781 cgcattcgcg ttgctgtggc tgcttggttg gaccccgggt gaacgcttcc tttcttcgat
    30841 taccggtttg acgttcgttg cctgcacctt tggaattgcg tcactcgtct ggagcgtcgc
    30901 gtctgggtca actgtcacgg tgagcttcgg caactggttc gccgtcggag actatcattt
    30961 tccgttggtg ctgatggcgg ataggctttc gttgcctttc ctggcgttga cggtcgtgct
    31021 atcgggactc atcgcgcagt tctcggcgac ttaccttcat cgcgagccgg gattcctgcg
    31081 attttttctt ctcctgcacc tctttgcctt cggatcctta ctcgcattcg ctgcgggttc
    31141 gtttgacctg ctcaccgcgg gatgggaagt ggtagggatc acttcggtct tgctcatcgg
    31201 tttttttcaa ttcaggccag ctcctgtgga aaatgggctc cgggtgttcg gcatatatcg
    31261 cgcctgtgac attgggttgt tcgtgggcgt gttcgccctg caccactggg ctgggacggc
    31321 gtcttttgaa agcggcctcc cagccctgac gggtacacag gcggtgatcg tatgcctcct
    31381 tctgctgctg gcggcggccg ggaaggccgc gcagattcct ttttcgggat ggctgccgcg
    31441 agctatggaa gggccgacgc catcgagtgc aatcttctac ggagcgattt ccatccatgc
    31501 gggtgcgtac cttctgctgc gagcgcaacc gctgcttgca aggtcgatcc tcgcttccgc
    31561 tctggtgata ctgattggtc tggctacggc aatccacggc accatcgttg gacgtgcgag
    31621 tgcggatgcc aagacgtcgc ttgcctatgc cactttaacg caagtcggcg tggtctttat
    31681 tgagattgga ctgggatgga aatggctcgc ggtggctcat attttgggaa atgccacggt
    31741 tcgtacgatg caatttctgc gcgcaccatc gatgcttcat gactatcacc aaatgcactc
    31801 ggcgatcggg ggcgacgtct cgccgaccgg caagcaaatc gaggaactac tcccggagcg
    31861 agtccagttg tggctctatc gctgggcttt cgatcgtggc cacctcgata caattctgga
    31921 ctgctgggtg attgatcctc tgctacaagt ctccacctat cttgcgaaag ccgatcgcgt
    31981 cggaatgcgt ttgactgcga tccggcgcgg caatgcggtg ccgatttcct tgcacggccc
    32041 ggcgaaacag gggaacgatt agtgtccatg cccggatggg tcctccatca accgcttctg
    32101 atcggtgtct ccgtcggcgt gccgtggttc gcagctgccc ttttaactgg ttatcgccat
    32161 cacgcgcgca tcgtcggttg tgcagctgca ttcgtatcga tcctcggaag cgctgtactt
    32221 ttagcggacg caccgaaggg cgagtcgttg taccaagcgc tgatgttgct ctattcctgt
    32281 ctgactttcg gcgcgacttt gctgcttcca cgccgcgatt gcacaacagg gacgatcagc
    32341 ggaatcctgt tcctgctcgg ttcgacgctc ttggcgtact cgaccggaaa tttacttgtg
    32401 ctgcttgccg catggatttt gtccactgtt ccgttcttat gggcggggtg gttccgtgct
    32461 cgtgcatggc gtccgctggc tggtcttctg ttatcaagtc tcgcccttgc cctggccgtt
    32521 ggactgattt cgactagcgc aggcgtcttt tccatcgata acctgaaagg gcaaagcccg
    32581 ggtggcgtag cagccttcgc gcttctcctc atagctgtgg tctttcgcaa gggaatttgc
    32641 cccgcccact cttgggtcgc agatgcggct gacaccggtc cagtagttcc gacggcgctt
    32701 ctgcttaacg ggcatttcgg agcactgctg gttgccaagt tgattgtgcc gttgtatcca
    32761 cgcacggaca gtttatttcc tgtgctttcc tatctcgcca ttgtgacggc ggtttatgtc
    32821 gcgattcgcg ctcttgcgga gaattctccc agccgactga tcgcctttct ggcgctcagc
    32881 cagtccgcct atatcttgag cgggctggaa agtcgaacag ctgaaggaat cgccggcgcg
    32941 atcgtgcatt ggttcgtggt tgcgatctcg acaatggctc ttttcggaat cctgcgattg
    33001 ctcgaagtgc gtttcggcga gaacctaacc gtgagtaagc acttcggttt ggccgagcac
    33061 gcgccccgcc tcgccgtgtc gtttgtgatc ttcgggctgg cgttagttgg tctgccgggc
    33121 acgctgggtt tctgttctca agaccttttg attcacgggg cgctcacatc gcaccgtctc
    33181 atcggattgc tgctacccat tgctacggcg atgaacgcga ttagcttctt ccggttattc
    33241 acgcgccttt tcttgggcaa gcgtcgaacc gggtttacgg tcatggcaga tgcattgccg
    33301 cgcgagcgct ggatacttgc tgccggtgtt ttgttcgtcg tcttcggggg ggtgttccca
    33361 aatgcggtcg taattcagcg ctcgtctgag gctgatatgg tgcaggaggc tattcacacg
    33421 gcggcggaac tgaaccctgt tgggaaggtt aaccggcatc cgggaaacta tcaatcattg
    33481 ccacgcgctg acgctggacc tatggctgct gaggcacatg gtcgcgctct aagttgggta
    33541 ttgagaacga cgttcgtcgg agccagccga tgagaaatga caagtttggc acgcatctta
    33601 cgtccggcaa tggtacgggg cggaaggatg cggtagctcg tggtctcgaa aagcagcagg
    33661 agcacacgcg gaatttgagg ttcggcattt tctatctggt ggttgggctt cttctctttc
    33721 tcgccatcaa tttgcaaagc agccgcaggc gaacggtaac cagcccaacg cgctctcgaa
    33781 cgtcggctta gatctgtccg taggacgtaa gccagagccg actggagctg ctccaattgt
    33841 gtcattcggg gagcgccgaa tcagagcacg cccacgaagt aggcgatcat gggctcttct
    33901 tctcaggtcc ggtatcacaa attcggatgg ttgcggggtg cgccgaagtc ctttgcgcag
    33961 aaaaagtttg gttgcggggg ttggatttga accaacgacc tccgggttat gagcaagatt
    34021 tcggactgta agtgccgtcg aatcaatcgc gctgttgcac gaattgacgc ttcggagcac
    34081 attctgtcag cttaaatgca gtagggaatt gggcttaatt cgatttgaaa gtttgtcagc
    34141 ttaaccgcag ttgcccgcgc cgtacaggtg cgctgattcc gcaatgttcc attgacttgc
    34201 gagctatcga ggcgtagggt ttgcgttgag cgggatgcgc cggtgcttga gacaccgaca
    34261 cgtccctagc cgccgaactt ccgaggaagc tgcgacggtt gcaggggatt gtaccatccg
    34321 gtctgggcaa acaggccgag tgctgcgcgt cccgcccatt cccaaaaggg gcgcgccgat
    34381 gtttccaccg ccaggctttc ccgccggatt ccagtacgcc gcaaaccctc ccggccacgc
    34441 agccgcaatc ctaggcgtcg tgctcgcggt cgcgctgtgc gttgcgcttg tatgggcact
    34501 ctggccgcgt agcatcggcg gaaaacagcg ccgtgagcgc aaaccgccga ggtaacgcag
    34561 cgtgagctat ctgaaacgcc accacgtggc accgaatctc ttcgcgagac cttcccgcat
    34621 aagaatcctg agcgcctcat cgtaagacgg cttgcatttg tcgggtaggt agcttttgag
    34681 tttcttgtca tcgaatgctt gcgtgcagcc tgcattattc agcgcttcca gcacacgctg
    34741 ccagagtata cgggctgcct cctccgcgcg cctctcaggt gctccgcgcc ggaactcaaa
    34801 gaagtctttc gccacgctta gcacaacgtc ccacataaac gcctcctgcg tactgcctgc
    34861 gcgtaacact gtgcgccact gaagcttccc ggtcaacgac ccctacttgc tttttcgcgc
    34921 cccaacacgc cagttcgcgc cccgcgaaaa ctcgatcctg atttgtcgcg ccgtggacgg
    34981 ttcgactaac atttcttcgc gcgatgggaa agcctaaacc gctgcccgga aactctcgcg
    35041 cggtgcgagc cgagcaagcc cgcaaattgc cgcgcaaatc gcgtgcaaat ccgctgcaaa
    35101 tccgcgcgaa attcgctagg gaatctcgct cgccttgcct cgttcggtgc cgcgcgcaat
    35161 cggtgccggg taattgggcg gcgcgtgccg tccacaatct gcccggctcg cttccacaat
    35221 cgttcgcttt tggaatggct cgcgtgacag tttgcggcca cgcatccaaa gggtttgcgc
    35281 gcgaaagttt tttggggttt gtggaagatt ccgcttgac
//
LOCUS       DATNDT010000158        21043 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_955432, whole genome shotgun sequence.
ACCESSION   DATNDT010000158 DATNDT010000000
VERSION     DATNDT010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 21043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21043
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_955432"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1399)
                     /locus_tag="VN788_05165"
     CDS             complement(<1..1399)
                     /locus_tag="VN788_05165"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU19955.1"
                     /translation="MFSRLRVLASRMRGFFSGHRLDEDFQRELASHLDMLTEENIRRG
                     LPPEEAKRQARLRPGGAAQLRETQHDVRGLPWLETFLQDIRFGLRILRKNPGFTVIAV
                     LTLALGIGANAAIFSVIDGALLDPVPFPEPQRLVNIYANWPHFDKAFLSYPNFLDLQR
                     DNSTFDQMAVWRIEWFTRTGQGDPQRLRGEMISANLLSVLGVRPLVGRTFRTDEDQLG
                     AAPVAMIGERLWKQDFGSDRNIAGQRIILDGKDYTIIGVMPSSIHLVRYQNSFFDDVY
                     PPVGQWDSKILRDRGFSVGLHAVGRIKRGMTIAQAGTDVDHLGKNLAAEFRNGNDGLN
                     IRIEPLSEDLAGNVRPTLLMLWAAVGFVLLIACANVANLLLARSTARSHEFAARAALG
                     AAQARIVRQFVVESALIVLGGGLAGVFLAGWASRVLLSMLPQALPASAHVAINWRVLL
                     FALATSVLAGILFGVV"
     gene            complement(1403..1747)
                     /locus_tag="VN788_05170"
     CDS             complement(1403..1747)
                     /locus_tag="VN788_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXU19956.1"
                     /translation="MPEAKSEILQGTLDLMVLKTLHAIGPLHGYGIARRLEQISEDVL
                     QLNQGTIYAALARLLQRGWISAAWGASENNRKARFYSITKAGQKQLAAETQNWKRMAG
                     VIGRVLQLSEKS"
     gene            2066..2461
                     /locus_tag="VN788_05175"
     CDS             2066..2461
                     /locus_tag="VN788_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19957.1"
                     /translation="MRIMRQVQGTSGFLGGRLLVEAEKVFWTLTAWEDGSAMNVFRTQ
                     GAHGEMMPKLLDWCDEAALVHWNQMTDVLPSWGEAHYRMMKEGRPSKVRHPSPAQVAY
                     EIPKPRAGRMERVLRPSRQGAESKASSRT"
     gene            complement(2508..3374)
                     /locus_tag="VN788_05180"
     CDS             complement(2508..3374)
                     /locus_tag="VN788_05180"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TylF/MycF/NovP-related O-methyltransferase"
                     /protein_id="HXU19958.1"
                     /translation="MPEAELYIDLLKRSVADALQERTWYVPIPRPKSLIKRVLSNLLA
                     ARGITLVREVDPSVRGEGSERPPRGTAWLDATSAFTLLGQDRLDNLQWCVEDVLRRKV
                     PGDMIETGVWRGGACILMRAILKAYGVRDRKVWAADSFEGLPPADPAQYPLDRHSTFH
                     EQRGFAVSLEEVRHNFDKYGLLDDQVEFVKGWFCDTLPKLAGHTWAVIHLDGDMYEST
                     AEGLENLYPGLSPGGYVIVDDFGAVPACRQAVLDFRKKYHIRDEMRVADWTGVYWQRD
                     STDVSVLMNRAS"
     gene            3558..4715
                     /gene="amrS"
                     /locus_tag="VN788_05185"
     CDS             3558..4715
                     /gene="amrS"
                     /locus_tag="VN788_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HXU19959.1"
                     /translation="MKVFGVSSMRDFTTLQEAAALHEARWWEPEPGTSGKGDKVHCYL
                     CPRHCHIGEGQAGFCFIRANHGGKLYSLGYAHPAALQIDPIEKKPLNHFLPGTRVFSM
                     GTAGCNMGCFFCQNWDISKSRADQVRSSHVPPEDVVALAIRNGCPTIAFTYNEPTIWG
                     EYVIDICRAAKEHGLKTVMVSNGYITREAFHDVYDHIDAANIDLKAFTENFYGRITLT
                     HLQPVLDTLQWLKNETNVWFEITNLLIPGLNDAPSETRALVDWVLEHLGPDIPLHFTA
                     FHPDFKLRDRAGTPPETLHSARAIALEAGLHYVYEGNIYSDGGNTTCPSCKTLLIRRS
                     WHDVRKNNLKNGCCPSCGRPIPGRWTNSKAPTVLSAEASTLISRAYGDLNV"
     gene            4708..5478
                     /locus_tag="VN788_05190"
     CDS             4708..5478
                     /locus_tag="VN788_05190"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HXU19960.1"
                     /translation="MSNAMKSVKLTAAASYLIEQKYDEYTPEQHAVWAELVRRRRPHI
                     ESFACREYLEGYEIIGLKADKLPNLAAISARLKPRTGWNATPVSGFLPSDAFFEMLAA
                     RMFPTTTKPRSRDSLEYTPEPDIFHDVFGHVPMHAHRVFADFLQHYGNVCAKIQDKET
                     LERLGRLFWYTVEFGLIREGAQTKMYGSGIISSQGECSNILEGGCEVRDFSLDEVLNT
                     PVKVDSIHHLLFAIESFDQIYEAMHEAEKRVLASPPTR"
     gene            5627..7186
                     /locus_tag="VN788_05195"
     CDS             5627..7186
                     /locus_tag="VN788_05195"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011446516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXU19961.1"
                     /translation="MSAVLPPNVSEKTFQKALDAFAGVVGKQWVLATDEDRRAYMDIY
                     AAGDADRHLPSAAVGPQSTEEVQAIIRIANEHKVPLWPISRGKNLGYGAAAPCMSGTV
                     VLDLGRMKRIIEVNERFGYCLIEPGVGFFDLFDYLQENKIPLWMSVPANAWGSVVGNA
                     LERGYGYTPYGDNTTHICGMEVVLPNGEMVRTGTGAMANSPCWQLFKYGFGPAWDQLF
                     VQSNFGVVTKMGLWLMPEPEATLGLAMQLQEPDDVGWAVDELAPLRLNGVLLQSPSIG
                     NSLRIAATLSQRSQWYDGDGAMPDDVLRQVLQKFGLGWWNIDLWLYGYDDVNEAHARI
                     IKNAFAKHTKQEFKVTKWHRGEPIANSGAGVPSTNALQIVNWRGGRGGHLGFSPVMPP
                     TSEHVLKQFHSTKKRYEEIGVDYYGSFTLMERYTTNVNLMVYDQNDSAMTAKVRALFK
                     VLVADAAKEGYSEYRTHLSYMDDVALTFDYNNHALLRLNETVKDALDPNGILAPGKQG
                     IWPKSIRSDKT"
     gene            7188..7616
                     /locus_tag="VN788_05200"
     CDS             7188..7616
                     /locus_tag="VN788_05200"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXU19962.1"
                     /translation="MQTFPGCIARLAIVIALGVFGAAHITAQEKAPDTSARKIAEGKA
                     LFNARCGYCHLAGGTGTLMLGRRLGKDKELLASRTDLSAEYIKRITRVGINSMPPHTR
                     IEVPDSELDLIAAYLTRPASERGASEPKADNLPGSYPVRE"
     gene            7609..8205
                     /locus_tag="VN788_05205"
     CDS             7609..8205
                     /locus_tag="VN788_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19963.1"
                     /translation="MNRRDLLKRAGAAIIPAAFLGSRLSAAGISRQIGTPRIAAVVYD
                     ERYSDCRSFADILVRHGATPFAVNSDCGRLWHGALRENPVWRAGSVAGLTTDSDFLVS
                     RACGREVGLGVSYEGAHDSRELNCVKHRFQSCAYPHEIAAALSNEDASWAESLAQSLV
                     RARWRNGTAIFRAPVWVETSRSHDHPGYLTSWLLEKST"
     gene            8285..8593
                     /locus_tag="VN788_05210"
     CDS             8285..8593
                     /locus_tag="VN788_05210"
                     /inference="COORDINATES: protein motif:HMM:NF022815.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3175 domain-containing protein"
                     /protein_id="HXU19964.1"
                     /translation="MVPKSKRWVAKVKTDSTHPPPGLFTKDASTIARVLASKKVSPKG
                     PQSGMRMLTYFINRGGRGLSRSRKRELERAKSLLSRRSRRTRGTRGTRRRSTARGRAS
                     "
     gene            complement(8637..9506)
                     /locus_tag="VN788_05215"
     CDS             complement(8637..9506)
                     /locus_tag="VN788_05215"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU19965.1"
                     /translation="MILITGASGTVGKAVLAEVARSGAKRRAMYRSKDEAAKAPAGTE
                     TVIADFSDKASLAPALRGVESVYLVCSPIPDLVQLEGNVIEACVSAGVSRVVLNSALG
                     AGDYSKSFPSWHRRVEDKLKASKLAHCILRPNSFMQNILAFYAPSIRAQGAFYAAMDN
                     ARTSYIDVRDVAAVAAKALQSREHDGQTYELNGPEALTCAEIAEKISRHAAVPAQFVD
                     IPVEAQRKAMLDQKMPEWQVTALLDLQAYYTGGQGGTVDGLLQKLLGRSPITMDHFLQ
                     EFAGEFRAHAAKA"
     gene            9800..11407
                     /gene="murJ"
                     /locus_tag="VN788_05220"
     CDS             9800..11407
                     /gene="murJ"
                     /locus_tag="VN788_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011164736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXU19966.1"
                     /translation="MTATATDPRARGASKRHAFLVASGILLSRIAGLIRERVFAHYFG
                     NSDAADAFKAAFRIPNFLQTLFGEGVLSASFIPVYAGLLARKDEEEARRTAGAIAGLL
                     ALTTSIIVLIGVLATPYLIDAIAFGFHGEKRELTIRLVRILFPGAGLLVFSALCLGVL
                     NSHRRFFLSYTAPVFWNIAMIVTMWGWGSRHAQFPLAVILAWGSVVGSGIQVAVQLPV
                     VLKLLRGLRLSLEHQSENVREVIRNFFPVFTGRGVVQISAYVDGLLATLLPTGAVAAL
                     AYAQILYTLPVSLFGMSVSAAELPHMSGALGDDREVAEALRARLDSGLRQIAFFVVPS
                     IAAFLFLGDVIVAAIYRTGQFKQADVIYVWGILAGATVGLLAQTLGRLYSSTYYALRD
                     TRTPLKFAIVRVALTTILGYLCAIPLPPAIGISSCWGVAGLTISAGVSSWVEFTLLRR
                     TLNQRIGRTGLAAGYVTKLWVAALAASAIGWLIHRHAGRHSAILIAILVLGAYGIAYF
                     AAAWVLGVREALGYFGRLIAPRGSKPG"
     gene            complement(11438..12172)
                     /locus_tag="VN788_05225"
     CDS             complement(11438..12172)
                     /locus_tag="VN788_05225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19967.1"
                     /translation="MRHAAFLLGAVLLFSTTSSAQDTSANPLLFAPFDPGLALAEFSS
                     VPTEPLVLASAAGSASTEPTAAPAIGAEPSSEPPAQQPRVFGVFETYKWQAYVGYAFF
                     RFFALPRQKENMNGIDLGAVYYPTASRIGVDGDILGQFGTFHNQSVNQSSKFTAYMGG
                     PRFRWQAPRGIELWAHGLAGFAKFLPQTAHGGQLAFSYEAGGGVDIGAFRRRIGYRIE
                     VDAVGTHFFHTNQVSPKITFGVVYKF"
     gene            12456..12935
                     /locus_tag="VN788_05230"
     CDS             12456..12935
                     /locus_tag="VN788_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HXU19968.1"
                     /translation="MSSIQSWEPERALERIRELQHLPGPLLPILHALQEEFGYIDKAA
                     IPLIADTLNISKAEVYGVISFYHDFRHESPGRHMLKMCRAESCQSMGCERMIRHVEDR
                     LGVKLGETTRDGGFTVEPVYCLGNCALSPAVMLDGKLYGRVSPEVADFLIDSAQRHA"
     gene            12932..14524
                     /locus_tag="VN788_05235"
     CDS             12932..14524
                     /locus_tag="VN788_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015832971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXU19969.1"
                     /translation="MSVKVYVPCDAAAVSVGADAVARAIAEQIASRRGDVSLIRNGSR
                     GMFWLEPLVEVETPRGRVAYGPVSADTVPSLFDSGFLEGKPHQLYLGLTEEIPFLKKQ
                     ERLTFARCGITDPLSLSDYMAHGGYRGLEKAIKLTPQAIVEEVTNSGLRGRGGAGFPT
                     GIKWQTVLNTPDDQKYIVCNADEGDSGTYADRMIMEGDPFVLIEGMTIAGMAVGATRG
                     YIYLRSEYPNAFRTLQRAIEIAHSKGYLGENVAGSGKSFDMDVRLGAGAYICGEETSL
                     LESLEGKRGQIRFKPPLPAIKGLFGKPTVVNNVISLATVPIILDKGGAYYRDFGLGKS
                     RGTLTIQLAGNIKHGGLVEKAFGLTLREAIYDYGGGTHSGRPLRAVQVGGPLGAYFPE
                     SLLDTPLDYEAFAEKKGMLGHGGIVVFDDTVDLAHQARFAMEFCAVESCGKCTPCRVG
                     STRGVEVIDKIIANEDRSKNLELLADLCEVMVDGSLCALGGLIPFPVQSAIRHFPEDF
                     DKPAPPPPVTNPSNTQESRSWT"
     gene            14515..17358
                     /gene="fdhF"
                     /locus_tag="VN788_05240"
     CDS             14515..17358
                     /gene="fdhF"
                     /locus_tag="VN788_05240"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HXU19970.1"
                     /translation="MDLIREIDYGTPKRVSEKNVTLEIDGKSVTVPAGTSLIRAAAEA
                     GIQVPKLCASDNLEPFGSCRLCLVEIDGRPGTPASCTTPALEGMKVRTQTPRLAKLRR
                     GVMELYASDHPMDWLNGGKTVSSEFQDMVEAVGLREVRYGRSGKNHQNAEKDDSNPYF
                     TFDPAKCIVCSRCVRACDEVQGTFALTIDGRGFGSMVSAGDKPFIESECVSCGACVQA
                     CPTNSLLEKSVMEKGEANRSVVTTCAYCGVGCSFKVELKGDEVVRMMPYKDGKANHGH
                     SCVKGRFAWGYATHKDRILKPMVRAKITDPWREVSWEEAIGRAASEFKRIQAKYGRDS
                     IGGITSSRCTDEETYLVQKMVRAAFGNNNTDTCARVCHSPTGYGMKTTLGTSAGTQNF
                     DSVEHADVIMVIGANPTDAHPVFASQMKRRLRQGAKLIVVDPRRIDLVKSAHVKADYH
                     LPLLPGTNVAMINAMAHVVVTEGLVDEPYVKERCDLVAFEGWRKFVAEERNSPEATEP
                     YTGVPAADVRAAARLYATGGNGAIYYGLGVTEHSQGSTMVMGMVNLAMATGNIGKEGV
                     GINPLRGQNNVQGSCDMGSFPHEFTGYRHVSETKVRESFEADWKVPLQSEPGLRISNM
                     IDAALDGTFKGLYIQGEDIVQSDPNTQHMSAGLSAMECVVIQDLFLNETASYAHVFLP
                     GSSFLEKDGTFINAERRIGMVRKAVPPMAGMADWEVTVALSNALGYPMHYNHPSEIMD
                     EIARLTPTFAGVSFEMLDRVGSVQWPCNEKAPMGTPIMHIGGMVRGKGRFMITEFIPT
                     DERVNARFPLILTTGRILTQYNVGAQTRRTDNNVWHDMDVLEIHPSDAEDRGIKDGDL
                     VSLASRSGDITLPAKVSERMQPGVVYTTFHHAMTGANVVTTEFADWATSCPEYKVTAV
                     QVQLARHISEWQEHYRKNRELTTRISHETPASD"
     gene            17361..17567
                     /locus_tag="VN788_05245"
     CDS             17361..17567
                     /locus_tag="VN788_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020163647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HXU19971.1"
                     /translation="MEAENMVHMANQIALFFASYPKDEAVASVTDHFKKFWEPRMRKQ
                     IIEYVAHGGTGLHDLALEAVKHLQ"
     gene            17581..18279
                     /locus_tag="VN788_05250"
     CDS             17581..18279
                     /locus_tag="VN788_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19972.1"
                     /translation="MKKQEQTAIEAVARRFSATWDKGSHPPDAYIMVAGKRVAVDVAT
                     LKRRGTGRGNTVKLHLRFDKVATRLIERLQATLGGTVPDGMTVLLTITAPIRLPSKTA
                     AALEDKIKTLLGRKSPGSDKKDTIHGNSFRIRLLRNDAERAPKMIGFVHNSDSDALLL
                     LNMTRELLERIGATAGRRATRLAGDRWLVLISPEGASGLQAYRYISSQLRMAAGFQKI
                     LMAFGDGRVEMLKG"
     gene            complement(18344..20599)
                     /locus_tag="VN788_05255"
     CDS             complement(18344..20599)
                     /locus_tag="VN788_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXU19973.1"
                     /translation="MASKTTDSSLASAVRPAAKVLVATTAMLAFISYWRAAAVVLNDL
                     GSSAFYAGAIAEHFIGKTAPWFVLAIMLFAFAVQQLYYESCGMFVRGGVYRVVKEGMG
                     STLAKFSVSALMFDYILTGPISGVSAGLYLVGLLNELLRYAHFNLVLPVNATAAAFAI
                     LVTLYFWWENIKGIPESSEKALRIMYVTTVMVVLMIVWCGCTLWVRGVHLPPWPHLSN
                     ITYSSDALGWLRHTSLPYTIGLIGILIGLGHSVLAMSGEETMAQVYREIEHPKLPNLK
                     KAGLVIFLYSMIFTAGVAFFAVMIIPDSVRGNFSDNPISGLAMHLVGPLTARIAFRIF
                     VVVVGVLMLAGAVNTAIVGSNGVLNRVSEDGILPSWFRHPHRRFGTSHRILNLVVGLQ
                     IFTIVLSRGDIFLLGEAYAFGVMWSFAMKGLAVLVLRYKQPGGREFRVPLNLKIANVE
                     IPVGVALITLTLFALCFINLFTKQVATVSGVAFTLIFFAVFEISEKIAKKESKSHAEL
                     DKFNLTQEGELTLENVGVRPGNILVPLSNYYALYPLEAALRRAKRREAEIVVLHVRML
                     RRAASGEYALAADQLFTNIEQLLFTKVLAIAEKEGKPVRLAVAAANDLWEGILRTAAN
                     LQSSSIVAGSSSNTSVTEQARDIGLAWERIPEPKPRVSLEVFSPSGREQIFYLGPHAP
                     RLTTKEIDLLHRVWLEFSDYLPGKEIHHHDIVHFALDEVEREICNEKKSEVLDRLKEH
                     LHEIENRRQHS"
     gene            20771..>21043
                     /locus_tag="VN788_05260"
     CDS             20771..>21043
                     /locus_tag="VN788_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HXU19974.1"
                     /translation="MKEFVPHRLLRNRHLMTIASAFLRRRFPRLPAAQPRLFDVEPET
                     QMRGHCYWHAEPRKHPTLLLVHGLEGSSDSAYILGCAEKGFVAGFNV"
BASE COUNT         4556 a         5912 c         6262 g         4313 t
ORIGIN      
        1 gaacaacacc aaagagaatt ccggccagca ccgaagtggc cagggcgaaa agcaaaaccc
       61 gccagttaat tgccacgtga gccgacgccg gcagcgcctg cggcaacata cttagaagca
      121 cccgacttgc ccaacccgcc aggaagacgc cggctaaacc tccgcccagc acgatcaagg
      181 cgctctcgac gacgaactga cgcacgatac gcgcctgggc agcacccagc gctgcacgtg
      241 cagcgaattc gtggctgcgc gcggtcgacc ttgcaagcaa aagattggct acattcgcgc
      301 acgcaatcaa aagcacaaac cctaccgcgg cccaaagcat cagcagcgtc ggccgaacat
      361 tgccagcgag atcttcggag agtggctcga tccgaatgtt cagtccatcg ttgccattgc
      421 ggaattcagc ggccaagttc ttccccagat ggtcgacatc tgtccctgct tgcgcgatgg
      481 tcatgccccg cttgatgcgg cctaccgcgt gcaagccgac gctgaagccc cggtcccgca
      541 gaatcttgct gtcccattga ccgacgggag ggtacacgtc gtcgaaaaag ctgttctgat
      601 accgcaccag atgaatgctc gacggcatga cgccgatgat cgtgtagtcc ttgccatcca
      661 aaatgatcct ttggccggcg atattgcgat ccgagccgaa atcttgcttc cacaaccgtt
      721 cgccgatcat cgcaacgggc gcggcgccga gctgatcttc gtctgtgcga aatgtccgtc
      781 ctacgagcgg acgcacgccc agcacggaaa gcaaattcgc cgaaatcatt tcgcctcgaa
      841 ggcgctgcgg gtctccttgc cctgttcgcg taaaccactc gattcgccaa acagccattt
      901 ggtcgaacgt gctgttgtcg cgctgtaggt ccaagaagtt agggtaggaa agaaatgcct
      961 tatcgaaatg cggccagttc gcatagatgt tcacaaggcg ttggggctcg gggaagggaa
     1021 ccgggtccag cagcgcgcca tcgatgacgc tgaatatcgc ggcattcgcg ccgatgccga
     1081 gagcgagagt gagtacggcg atgacggtga aaccaggatt cttgcgcagg atgcggaggc
     1141 cgaagcggat gtcttgcagg aacgtttcca gccagggaag gccgcggacg tcgtgctgcg
     1201 tctcgcggag ttgcgcggcg cctccggggc gaaggcgcgc ttggcgtttg gcttcctcgg
     1261 gcgggaggcc gcggcggatg ttttcctcgg tgagcatgtc gaggtgagag gcaagctcgc
     1321 gttgaaaatc ttcgtcgagg cgatggccgg agaagaagcc gcgcatgcgg gaagccaaga
     1381 cacgcagcct ggaaaacata ggttagctct tttccgaaag ctggaggacg cggccgatga
     1441 cgccggccat gcgtttccag ttttgcgttt cggcggcgag ctgcttctgg ccggctttgg
     1501 tgatggagta gaagcgagcc ttgcgattgt tttccgacgc gccccaggcc gccgaaatcc
     1561 agccgcgctg cagcagccgg gcgagtgccg cataaatggt gccctggtta agctgcaaca
     1621 cgtcttcgct gatctgttcg aggcggcgag caatgccgta gccatggagc gggccgatag
     1681 catggagagt tttcagtacc atcagatcga gggtgccttg caggatttcg gatttggctt
     1741 cgggcatggg ttctcctgtg tgttggccac aggagaatgg catacgtcct gtaggatgtc
     1801 aacaggaacg cggaacgtga cagcccgcgt gcggttcttg aaaaaagaaa ccagaaattg
     1861 gaaataacag ggtggagatt gaaactcata aatcgaaact ggcaactggg cgattcgaaa
     1921 atcggacgcg agcctcgctt ccttttgagc gtgcgagcca tcgaatagaa gtggcatcag
     1981 gctaaggagc gtcatggttc tcgtttctgt aacgcgcttg cgggtgcgtt cctcgtggta
     2041 tctcgcgtcg tttttctgga gaaacatgag gattatgcgc caagtgcagg gcacctcagg
     2101 gtttcttggc ggcaggctgc ttgtcgaggc cgagaaagtc ttttggacat tgacggcttg
     2161 ggaagatgga tcggcgatga atgtgtttcg cacgcagggc gcgcatggag agatgatgcc
     2221 gaagctgctt gactggtgcg acgaagcggc tttggtgcat tggaaccaga tgacggacgt
     2281 gcttccctcg tggggggagg cccactatcg catgatgaaa gaaggcaggc cgtcaaaagt
     2341 caggcatccc tctcccgctc aggtggcgta tgaaattccg aagccgcgcg cggggcgcat
     2401 ggagcgagtt ttgcgtccgt cacgccaagg tgcggagtca aaagcatcaa gccgaaccta
     2461 aaaatcgtgc cgcccgctgc aatcgagttg cgtttggttc gggttcgtca ggatgcgcga
     2521 ttcatcagaa cggagacgtc cgtcgagtcc cgctgccagt acactcccgt ccaatccgcg
     2581 acgcgcattt catctctgat atgatatttc ttgcggaaat ccaagactgc ctggcgacat
     2641 gcaggcaccg ctccgaaatc gtccacgatg acgtaacccc ccggcgagag tccgggatag
     2701 aggttttcga ggccctcagc cgttgactca tacatgtcgc catcaaggtg gatcaccgcc
     2761 caggtatgcc ccgccagctt cggtagcgtg tcgcaaaacc agcctttcac aaactctacc
     2821 tgatcatcta atagtccgta cttgtcgaag ttgtgccgga cctcttcgag cgatactgcg
     2881 aatccacgtt gctcgtgaaa tgtgctgtgc cggtcaagag gatactgagc agggtcggcg
     2941 ggcggtaagc cctcgaagga atccgcggcc cagactttgc ggtcgcgcac accataagct
     3001 tttaagattg cacgcatgag gatgcatgcg ccaccccgcc acacgccggt ctcgatcatg
     3061 tctccaggaa ctttgcgccg gagcacatcc tcaacacacc attgaagatt gtccaggcga
     3121 tcttgtccca gcagagtgaa cgctgaggtc gcgtcaagcc acgcggttcc gcgcggcggt
     3181 cgttcggatc cttcgccgcg aacactagga tctacctcgc gcaccagagt tattcctcgg
     3241 gccgcgagca gattggaaag cacccgtttt atcaagcttt taggtctggg tattggcaca
     3301 taccatgttc gctcctgcag ggcatcggcg acggatcgct tcagaagatc tatgtatagc
     3361 tccgcctccg gcattggcct cgcctccaag tccggatttc aagttattaa taatccgagc
     3421 atcaatgtag cctcctgtta atcgatgagt ccatacaatt aaaacggtag atacttcaaa
     3481 aaatcagtgg acattttttg gcagcgcgaa aagtgaaagc tggtgaaacg ccgctcacca
     3541 tacggaagag gcgtataatg aaagtcttcg gggtttcgtc catgcgcgac tttacaacac
     3601 tccaagaggc ggcggctctt cacgaggctc ggtggtggga gccggagccc ggtacctccg
     3661 gcaagggcga caaggtgcat tgttacctgt gtcctcggca ttgccatatt ggcgaggggc
     3721 aggcgggatt ctgtttcatt cgcgcgaatc atggcgggaa actttacagc ctgggctacg
     3781 cgcacccagc cgcgctacag atcgatccca tcgagaaaaa accgctgaac cattttcttc
     3841 cgggcacgcg cgttttttcc atgggcacgg ccggctgcaa catgggctgc tttttctgcc
     3901 agaactggga catctcgaaa tcgcgcgccg atcaggtgcg gtcgtcgcac gtaccgccgg
     3961 aggatgtggt ggcactggcg attcgcaacg ggtgccctac gatcgcgttc acctacaacg
     4021 agccgacgat ctggggcgag tacgtgatcg atatttgccg cgccgctaag gaacacggcc
     4081 tcaagacggt gatggtttcg aacggctaca tcacccgtga ggcttttcac gatgtttacg
     4141 atcacattga cgccgcgaat atcgacctga aggcgttcac cgaaaatttt tacggacgca
     4201 ttacgctgac gcatctgcag ccggtgctcg acactcttca atggctcaag aacgaaacga
     4261 atgtgtggtt cgagattacc aacctgctga ttcccggact gaacgatgcg ccgagcgaga
     4321 cccgcgcgct tgtggattgg gtgctggagc atctgggtcc ggatattccg ctgcacttca
     4381 ctgcgtttca tccagatttc aaattgcgag atcgggcggg tacgccgccg gaaacgctgc
     4441 actcggcgcg cgcgatcgcg ctggaagcgg gactgcatta cgtttatgaa gggaatattt
     4501 acagtgacgg cggaaacaca acctgtccgt cgtgcaagac tttgctgatc cgccgatcgt
     4561 ggcatgatgt gcggaaaaat aatttgaaaa atggatgctg cccgagctgc ggccggccaa
     4621 tcccggggcg gtggacgaac tcaaaagcgc cgacagtgct ttcagctgag gcatctacgc
     4681 tgatcagtcg agcttacgga gatctcaatg tctaacgcaa tgaagagcgt taagctcacg
     4741 gctgccgcgt cgtatctgat cgagcagaaa tatgatgagt acactccgga acagcacgcg
     4801 gtgtgggcgg aacttgtccg gcggcgacgg ccgcacatcg agagctttgc ttgccgcgag
     4861 tatcttgaag gatatgaaat tatcggactc aaagccgaca aacttccaaa tctcgccgcg
     4921 atcagcgcgc gattgaaacc gcgcacgggt tggaacgcca cgccggtgag tggctttctg
     4981 ccgagcgacg cgttttttga gatgctcgct gcgcgcatgt ttcccaccac cacgaagccg
     5041 cggagccgcg actcgctcga atacactccc gaacccgaca tttttcacga tgttttcggc
     5101 catgttccta tgcacgcgca tcgtgtgttc gctgatttcc tgcagcacta cggcaacgtg
     5161 tgcgccaaga ttcaggataa ggaaacgctg gaacggttgg ggcggctttt ctggtacacg
     5221 gttgaatttg gcctgattcg agaaggcgcg caaacgaaga tgtacggcag cggcattatc
     5281 agttcccagg gagagtgttc gaatatattg gagggaggct gcgaggtgag ggatttcagc
     5341 ctcgatgaag ttctgaacac cccggtgaag gtggatagca tccaccacct gctttttgcc
     5401 atcgaaagct tcgatcaaat ctacgaagcc atgcatgagg ctgaaaagcg cgtgctggcg
     5461 tctccgccga cgagatgatt tcgttttcgc cgcgcggcaa atccggcaat cgacaaattc
     5521 attgacggaa aaattcccgc gtgctatcca cgcattcgcg ccgcccattt gtttttgtgt
     5581 tacgcgggat tcaccagccg gcgcacgcaa gggagaatga ttgcccatgt ccgctgtgct
     5641 gccgccaaac gtatccgaaa aaacttttca gaaagcgctg gacgcgttcg ccggagtggt
     5701 ggggaagcaa tgggtgctgg ccaccgatga agatcgccgc gcgtacatgg atatttatgc
     5761 ggcgggcgat gccgaccggc atcttcccag cgcggcggtg ggtccgcaat cgaccgagga
     5821 ggtgcaggcc atcattcgca tcgcgaatga acacaaggtg ccgctctggc cgatttcgcg
     5881 cggcaaaaac ttgggatatg gcgcggcggc gccgtgcatg tccggcacag tggtgctcga
     5941 tctggggcgg atgaagcgga ttatcgaagt gaacgagcgc ttcggctact gcctgatcga
     6001 gccgggcgta ggcttcttcg atctgtttga ctatttgcag gaaaataaaa tcccgctatg
     6061 gatgtcggtt cccgcgaacg cgtggggcag cgtggtgggc aacgcgctgg aacgcggcta
     6121 cggctacacg ccctacggcg acaacaccac tcatatatgc ggcatggaag ttgtgcttcc
     6181 gaatggcgaa atggttcgca ccggcactgg cgcgatggcg aacagcccgt gctggcaatt
     6241 gttcaaatat gggttcgggc cggcgtggga ccaattgttc gtgcaatcga atttcggcgt
     6301 ggtgacgaaa atgggcttgt ggctgatgcc ggagcctgag gccacgctgg gcctcgcaat
     6361 gcagttgcag gaaccggacg acgtaggctg ggcggtggat gaacttgcac cgctgcggct
     6421 gaacggagtg ctgctgcaaa gtccgagcat cggcaattcg ctgcgcattg ccgcgacgct
     6481 gtcgcaacgc agccaatggt atgacggcga tggagcgatg ccggatgatg tgcttcggca
     6541 ggtgctgcag aaatttgggc tgggctggtg gaacatcgac ctgtggcttt acgggtacga
     6601 cgatgtgaac gaagcgcatg cgcgcatcat caagaacgcg ttcgccaagc atacgaagca
     6661 ggagttcaaa gtgacgaagt ggcatcgtgg cgaacccatc gccaattccg gagctggcgt
     6721 tcccagcacg aacgcgttgc agatcgtaaa ttggcgcggc ggccgcggcg ggcatctcgg
     6781 gttctcgcca gtgatgccgc cgacaagcga gcacgtgttg aagcagtttc atagcaccaa
     6841 aaaaaggtac gaggaaattg gagttgatta ctacggctcg ttcacgctga tggaacgcta
     6901 cacgacgaac gttaacttga tggtttacga ccagaacgat tcggcgatga cggccaaagt
     6961 gcgcgccctc ttcaaagttc tggtggcgga cgcggcaaaa gaaggctact ccgaataccg
     7021 aacccacctt tcctacatgg acgatgtggc gctcacgttc gattacaata atcacgcctt
     7081 gctgcggctg aatgaaacgg tgaaagacgc gctggatccc aatggcatcc tcgcgccggg
     7141 gaagcaagga atttggccga agtcaattcg gagcgataag acgtaatatg cagacatttc
     7201 caggatgcat cgcaagattg gcgattgtga ttgcgctggg tgttttcggc gcggcgcaca
     7261 tcacggccca agagaaagcg ccggacactt cagcgaggaa aatcgcggaa gggaaagcgc
     7321 tcttcaacgc gcgctgcggc tattgccatc tcgccggagg gacgggcacg ttgatgctcg
     7381 gacggcggct gggcaaggat aaggagctgc tcgcgtcacg cacggattta agcgccgagt
     7441 acattaagag aattacccgc gtgggaatca acagcatgcc gccgcatacg cgaattgaag
     7501 ttcccgatag cgaactggat ttgattgcgg cgtacctcac gcgtccggca tccgagcgcg
     7561 gcgcttctga acccaaagct gacaatttgc cggggagtta tcctgtccgt gaatagacgc
     7621 gatcttttga agcgagctgg ggccgcgata attcccgctg catttctggg gtcgcggctt
     7681 tcggcggcgg gtatttcgcg gcagatcgga actccgcgta ttgcagcggt tgtgtatgac
     7741 gagcggtata gcgactgccg atcgttcgcc gatattcttg tgcgacacgg cgcaactccg
     7801 ttcgcagtga acagcgattg cggcaggctt tggcacggcg cgctgcgaga aaatccggtg
     7861 tggcgtgccg gaagcgttgc cggcctcacg acagattcag atttcttggt atcgcgcgcg
     7921 tgcggaaggg aagttggcct cggtgtcagt tacgagggcg cgcacgactc tcgcgaatta
     7981 aattgcgtca aacaccgctt ccaaagctgc gcttacccgc acgaaatcgc cgctgcactc
     8041 agcaacgaag acgcatcctg ggccgagtcg ctcgcgcaat cgttggttcg cgcgcggtgg
     8101 cgcaacggca ctgcaatttt tcgcgcgccc gtatgggtag agacctctcg ctcgcacgac
     8161 caccctggct acctgacttc ctggctcctc gaaaagtcca cgtaacctta ccgctccgac
     8221 gctaagtcat tcgctgtatt gatccgtgtg cggcgtcgcg ctaattacaa tgggggagga
     8281 atacatggtg ccgaaatcca aacgatgggt agcaaaggtt aagacggact caacgcatcc
     8341 gcctccaggg cttttcacga aggacgcctc cacgattgcg cgagtgctcg cttcaaagaa
     8401 agtttcgccc aaggggcctc agtccggcat gcggatgctg acgtatttca taaatcgggg
     8461 cgggcgagga ttaagccggt cgcgcaagag ggagctcgaa agagcgaaat cgttgctctc
     8521 gaggcgaagt cggagaacgc ggggaacacg gggaacacgg agaagaagta ccgcaagggg
     8581 cagggcctcg tgagctagtc cacacgatta ctgcgttcgc gtacgcggcg tgtcctttat
     8641 gctttcgcgg catgagcgcg gaactcgccg gcgaattcct gaaggaaatg gtccatcgtg
     8701 attggagaac gtccaagcag cttttgcagc aggccatcta ccgttccgcc ctggccgccg
     8761 gtgtaatatg cctgcaaatc gaggagggcg gtgacctgcc actccggcat tttctgatcg
     8821 agcatggcct tccgctgggc ttccaccgga atgtcaacaa attgagctgg gaccgccgcg
     8881 tggcgcgaaa ttttctcggc aatttccgca caggtgagcg cttccggccc attcaattcg
     8941 tacgtctggc catcgtgttc gcggctttgc aacgccttgg ccgctacggc ggcgacgtcc
     9001 cgcacatcga tgtagcttgt gcgcgcattg tccatcgcgg cgtaaaacgc accctgggcg
     9061 cggatgcttg gcgcgtaaaa cgccagaatg ttctgcatga agctgttcgg gcgaagaatg
     9121 cagtgcgcca gcttcgacgc tttcagcttg tcttcaactc tgcgatgcca gctcggaaag
     9181 gattttgagt aatctcccgc acccaatgct gaattcagca cgacgcgact tacgcccgcg
     9241 gagacgcagg cttcgataac gtttccctcc aattgcacga gatccggaat cggcgagcag
     9301 accagataaa cgctctcgac tccgcgcagc gctggcgcga ggcttgcttt atcggaaaaa
     9361 tccgcaatca cggtttcggt tcccgccgga gctttggccg cttcgtcctt ggaacggtac
     9421 atcgcgcggc gtttcgcgcc actgcgcgcc acctcagcca agacggcttt gccgacggtt
     9481 ccgcttgcac cagtgattag gatcatttct tcctcccggg agggatgctc tccagacagg
     9541 atgaaggcga aacgagccgc gaaagcctac gtcctgcccc gtagggaatg gtataaccat
     9601 ccgcagcgaa ccggaagtac gcacaatcgt gtaaccctga gtctccatcg agaaccgtca
     9661 tggcttcccg cgcaggtgac tacggctgaa tgttccgcgc acacagagca acagcagccg
     9721 gaatccaagt tgacatctca gcaggtacct tttgtaatga attgtttcag ccaatgtaca
     9781 cgcccgttcc atcctgctcg tgacggccac ggcgacggac ccgagagccc gaggggcgtc
     9841 gaagcgccac gcttttctgg tcgcatcggg tattttactc agccggatcg cgggcctgat
     9901 ccgggaacgc gtgttcgcgc attacttcgg caactccgac gcggccgacg cttttaaagc
     9961 cgccttccgc attcccaatt tcctgcagac gctgttcggc gaaggggtgc tttcggcttc
    10021 atttattccg gtgtacgccg gtttgctggc acggaaagac gaggaggagg ctcggcgcac
    10081 cgccggcgcc attgccggtc ttttggcgct gaccacttcg atcatcgtcc taatcggcgt
    10141 gcttgcgacg ccctacctga ttgacgcaat cgcttttggc tttcacggag agaagcggga
    10201 gctaacgatt cggctagtgc gcatcttgtt tccaggcgcc ggcctgctgg ttttctccgc
    10261 gctatgcctg ggcgtcctca atagccaccg gcgattcttt ctttcctaca ccgcgccggt
    10321 cttctggaac atcgcgatga tcgtaaccat gtggggatgg ggatcacggc acgcgcagtt
    10381 tccgctggcc gtgattctgg cgtggggatc ggtggttggc agcgggatac aggtggccgt
    10441 tcaattgccg gtggtgctga agctcctgcg cggcctgcgt ctttcgctgg aacaccagag
    10501 cgaaaacgtg cgcgaggtga tccgcaattt ctttcctgtg ttcaccggcc gcggcgtggt
    10561 gcaaatcagc gcgtacgtgg atggattgct agccacgctt ctgcccacgg gcgccgtggc
    10621 tgctttagcc tacgcgcaga ttctgtacac gcttcccgtg agcctcttcg gaatgtcggt
    10681 ttctgccgct gaactgccgc acatgtccgg cgcgctcggc gatgatcggg aggtggcgga
    10741 agcgcttcgc gcacgcctcg attccggctt gcgccagatt gcgtttttcg tggttccatc
    10801 catcgctgca ttcctatttc taggcgatgt gattgtggcg gccatctacc ggacggggca
    10861 attcaagcag gccgatgtga tctatgtttg gggaatcctg gcgggcgcca cggtcggact
    10921 gctcgcgcaa acactgggcc ggttatattc ctcgacgtac tacgcgctgc gcgatacgcg
    10981 cacgcccctg aaatttgcga ttgtgcgagt cgccttgact acgattctcg gctatctgtg
    11041 cgcgattccg ttgccgcctg ccattggaat cagctcttgc tggggcgtgg ccggtctaac
    11101 aatttcagct ggggtatctt cgtgggtcga gttcacttta ttgcgacgca cattgaacca
    11161 gcggatcggg cgaaccggac tggcagcagg ctatgtgacg aaattatggg tggctgcgct
    11221 tgctgcgtcc gcaatcggct ggctgataca ccgacacgcg ggccggcatt ccgcgatttt
    11281 gattgccatt ctggtgctcg gggcgtacgg gatcgcgtat ttcgctgcgg cgtgggtgct
    11341 gggagttcgc gaagcgctgg gatacttcgg caggttgatt gcaccacggg gatccaagcc
    11401 cggctagcac ggcaaggacg gcgttgaaat ttcgcggtta gaacttgtaa acgacaccga
    11461 atgtaatctt cgggctcacc tgattcgtgt ggaagaaatg agttccgacc gcgtcgacct
    11521 caatcctgta gccgatgcgc cggcgaaacg ctccaatgtc caccccgcct ccggcttcat
    11581 aggagaaggc aagctggccg ccatgagctg tttggggaag gaacttcgcg aacccagcta
    11641 agccgtgggc ccaaagctcg atgccgcgtg gggcctgcca tcggaagcgc ggaccgccca
    11701 tgtaggctgt gaattttgag ctctgattca cactctgatt atgaaatgtt ccaaattggc
    11761 cgaggatgtc gccgtccact ccaatccgcg aggcagtcgg ataataaacg gcccctaggt
    11821 cgatgccgtt catgttttcc ttctggcggg gcagcgcaaa gaaacgaaaa aatgcgtagc
    11881 cgacgtatgc ctgccactta taggtttcga atacgccgaa aactctcggt tgttgggctg
    11941 gcggctcgct ggatggttca gctccaatgg ccggggccgc cgttggctcg gtgctggccg
    12001 acccagcggc ggacgcaagg accagcggct cagtgggaac tgacgaaaac tcggcgagag
    12061 ccaggccggg atcgaatggc gcaaataaga gcggattcgc ggaagtatcc tgagcggaac
    12121 tggtggtaga aaatagaagg acggccccta gcaagaatgc agcgtgtctc atcgcgtgtc
    12181 tcccttgagc tgagctgccc gcctcggtct tgcgccttct aatttggctg agaatagggc
    12241 gaggtgtcca ccctaaattc aatgtaggcg ggaccgagcg aggacctctc ccggcgggtg
    12301 ggctagccga gggatcgcgc gaagcattcg gcgacgggag caaagtaaaa accggggctt
    12361 agcagcgaat ggcgtgtgtt agaatattgg ggaaatggca gtgagaatag gcatttgaag
    12421 tagctctcta accacgaagc gggagttcgc cgcacatgag cagtatccaa agttgggaac
    12481 ctgaaagggc tttagaacgt attcgggagt tgcagcacct gcctgggcct ctcctcccga
    12541 ttttgcatgc attgcaagag gaatttggat acatcgacaa ggcggcgatc ccgctgatcg
    12601 ccgacacgct gaacatttcc aaggccgaag tgtacggcgt gatcagtttt tatcacgatt
    12661 ttcgtcacga atctcccggt cgtcacatgc tgaaaatgtg ccgcgcggaa tcgtgccagt
    12721 caatggggtg cgagcgcatg attcggcacg tggaagatcg attgggtgtg aaactggggg
    12781 agaccacgcg cgacggcggc ttcaccgtgg agccggtgta ttgcctgggc aattgcgcac
    12841 tgtctcccgc ggtgatgctc gacggcaaac tttacggccg agtctcgccg gaagtagcgg
    12901 actttctaat cgatagcgcg cagaggcacg catgagcgtc aaagtctatg tcccgtgcga
    12961 cgctgcggct gtttccgtcg gagcggatgc cgtcgccagg gcgatcgcgg agcaaatcgc
    13021 ttcgcgtcgg ggcgatgtct cgctgattcg caatggctcg cgcggcatgt tctggctgga
    13081 accgctggtc gaggtggaaa cgccgcgtgg ccgcgtagcc tacggaccgg tttccgcgga
    13141 cacagttccg agcctgttcg attccggctt tctggaagga aagccgcacc aactttatct
    13201 cggccttacg gaagaaattc cgtttctcaa gaagcaggaa cggctcacct tcgcgcgctg
    13261 cggaatcacc gatccgcttt cgctttccga ttacatggcg cacggcggat atcgcggcct
    13321 cgaaaaagcc atcaagctca cgccgcaggc gattgtggaa gaagtcacaa actccggcct
    13381 tcgcgggcgc ggcggagcgg gctttcccac aggaatcaaa tggcaaaccg tccttaatac
    13441 tccggacgat caaaaatata ttgtgtgcaa tgcggacgaa ggcgacagcg gcacctacgc
    13501 cgaccgcatg atcatggaag gcgatccctt tgtcttgatt gagggaatga ccatcgccgg
    13561 aatggccgtc ggcgccacac gcggctacat ctatctgcgc tcggaatatc cgaatgcgtt
    13621 cagaaccctg cagcgcgcaa ttgagatcgc tcacagcaaa ggctacctcg gcgagaatgt
    13681 cgcaggcagc ggcaagagct tcgatatgga cgtgcgctta ggcgcgggtg catatatctg
    13741 cggcgaggaa acctcgctgc tcgaaagcct ggaagggaag cgcggacaga ttcgtttcaa
    13801 gccgccgctg cccgcaatca aaggactttt cggcaagccg acggtggtca acaacgtgat
    13861 ttcgcttgcc acagtcccga ttattctgga caaaggcggc gcgtactatc gcgatttcgg
    13921 cctgggcaaa tcacgcggca cgctgaccat ccaactcgcg ggaaatatca agcatggggg
    13981 cctggtggaa aaagcctttg gcctcacgct gcgcgaggca atttacgatt atggcggcgg
    14041 gactcacagc ggacggccgt tgcgcgccgt gcaggtcggc gggccgctcg gcgcgtattt
    14101 ccccgaatca ttgctggaca ctccgctcga ttacgaagcc ttcgctgaaa agaaaggtat
    14161 gctcggccac ggcggtatcg tcgttttcga cgatacggtt gatctcgcgc accaggcgcg
    14221 cttcgcgatg gaattttgcg ccgtggaaag ctgcggcaag tgcacgccct gccgcgttgg
    14281 ctccacgcgc ggtgtagaag taatcgacaa gatcatcgcc aatgaagatc gctcgaaaaa
    14341 tctcgagctg ctcgccgatc tttgcgaggt aatggtagat ggttcgctct gcgctctcgg
    14401 cggactcatt ccttttcccg tgcagagcgc gattcgccat tttccggaag attttgataa
    14461 gccggcgcca cccccgccgg tcactaatcc aagcaatact caggagtcgc gatcatggac
    14521 ttgattcgag aaatcgatta cgggacgccc aagcgcgttt ccgaaaaaaa cgtcacgctg
    14581 gaaatcgatg gcaaatccgt caccgtgccc gcgggcacct cattaattcg cgccgcagcc
    14641 gaggctggca tccaagtgcc caagctttgt gctagcgata acctcgagcc atttggttcc
    14701 tgtcgcctct gcttggttga aattgatgga cgccccggga ctcccgcgtc ctgcaccacg
    14761 cccgccctag agggaatgaa agttcgcacc caaacgcctc gccttgccaa gttgcgtcgc
    14821 ggcgtgatgg agctgtacgc ctccgatcac ccgatggatt ggctgaacgg cggaaaaacc
    14881 gtgagttcgg aatttcagga tatggttgag gcggtcggct tgcgcgaggt ccgctacggc
    14941 cgctccggta agaatcacca gaacgccgaa aaggacgatt cgaatccgta tttcaccttc
    15001 gatccagcga agtgcatcgt gtgctcgcgc tgcgtgcgcg catgcgacga agtccaagga
    15061 actttcgcgc tcaccatcga cgggcgcggc tttggttcca tggtctccgc tggcgataag
    15121 cctttcatcg aatcggagtg cgtttcctgc ggcgcctgcg tgcaggcttg tcccacgaat
    15181 agccttctgg aaaagtcagt catggaaaag ggcgaggcca accgcagcgt cgttacaaca
    15241 tgtgcgtatt gcggcgtggg ctgctcgttc aaagtagagc tgaaaggcga cgaagttgtt
    15301 cgcatgatgc cctataagga cggcaaagcg aatcacggcc actcttgcgt gaaaggccgc
    15361 tttgcctggg gctacgccac gcacaaagac cgcattctca agcccatggt ccgcgccaaa
    15421 attaccgacc catggcgcga agtaagttgg gaagaagcta tcggccgagc cgccagcgaa
    15481 ttcaagcgta tccaggcgaa atacggacgc gattcaatcg gcggcattac ctcctcgcgc
    15541 tgcacggacg aagaaaccta cctcgttcaa aagatggtcc gggcggcctt cggcaataac
    15601 aatactgata cgtgcgcccg cgtctgccat tcccccacgg gctatggcat gaagacgact
    15661 cttggaacct ctgccggcac gcagaatttc gattccgtgg aacatgccga cgtgattatg
    15721 gtcattggcg cgaatcccac cgacgcgcat ccggttttcg cttcgcaaat gaagcgccgg
    15781 ttgcgccagg gcgccaagct catcgtcgtt gatccgcgcc gcattgacct agtgaagtcg
    15841 gcgcacgtca aagccgatta tcatctgcca ctgctgccgg gcaccaacgt cgccatgatt
    15901 aacgcgatgg cgcacgtggt ggtcaccgag ggcctggtgg atgagccgta tgtgaaggag
    15961 cgttgcgatc tggttgcctt cgaagggtgg cgcaagtttg tcgctgagga acgcaattca
    16021 ccggaagcca ccgagcctta cactggagtg cctgccgcgg acgttcgcgc ggcggcgcgc
    16081 ctgtacgcca ccggcgggaa tggagccatc tactatgggc tcggcgtcac cgaacacagc
    16141 cagggctcca cgatggtgat gggtatggtc aatctcgcga tggccaccgg taatatcggc
    16201 aaggaaggcg tcggcatcaa tccattgcgc ggacagaaca atgtgcaggg ctcctgcgac
    16261 atgggttcat tcccgcacga gttcacggga tataggcacg tgtccgaaac taaagtgcga
    16321 gaatcgttcg aagccgattg gaaagttcct cttcaatcgg aaccgggcct gcgcatttca
    16381 aacatgatcg acgcggcatt ggacggcacc ttcaagggcc tctatatcca gggcgaagac
    16441 atcgttcagt ccgatcccaa cacgcagcac atgtccgccg gtttgtctgc catggaatgc
    16501 gtggtgatcc aggatttgtt cctcaacgaa accgccagtt acgctcatgt gtttctgccc
    16561 gggtcgtctt tcctcgaaaa agacggcacg tttatcaacg cggaacgccg catcggcatg
    16621 gtgcgaaagg ccgttcctcc catggccgga atggccgatt gggaagtcac cgtcgcgctc
    16681 tccaacgccc ttggctaccc gatgcattac aaccatccct ccgaaattat ggacgagatc
    16741 gcgcgcctca cgcctacctt cgcaggcgtc agcttcgaga tgctcgatcg cgttggcagt
    16801 gtccaatggc cgtgcaacga gaaggctccc atgggcacgc ccatcatgca cattggcggc
    16861 atggtgcgcg ggaagggccg cttcatgatt acggaattca ttcccaccga cgagcgcgtc
    16921 aacgcacgct ttccgctcat tctcacgacc gggcgcatcc tcacgcagta caacgtgggt
    16981 gcgcaaacgc gccgcacgga taacaacgtt tggcacgata tggatgtgct cgagattcat
    17041 ccttccgatg cggaagatcg cgggatcaag gacggagacc tggtctcact ggccagccgc
    17101 tcgggcgata tcacgctgcc cgcgaaagtc tctgagcgca tgcagcccgg cgtggtctat
    17161 accacgttcc accacgccat gaccggcgcg aacgtggtga ccacggagtt tgccgattgg
    17221 gccacaagtt gtcccgaata taaagtgacg gcagtgcagg tgcagctcgc gcgtcacatc
    17281 tccgaatggc aggagcatta tcggaaaaac cgagagctga ccacgcgaat ttcgcacgaa
    17341 actcccgcga gcgactagcc atggaagccg aaaacatggt tcacatggcg aaccagatcg
    17401 ccctcttctt cgccagctac ccgaaggatg aggccgtggc cagcgtcacc gaccacttca
    17461 aaaaattctg ggaaccgcgc atgcgcaaac agatcatcga atacgtcgcg cacggcggca
    17521 caggtctcca cgacctggct ctcgaagccg taaaacacct ccagtaagcg cagctccgac
    17581 ttgaaaaagc aggagcaaac cgccatcgaa gccgtcgcca gacggttctc ggcaacgtgg
    17641 gacaagggta gtcatcctcc cgacgcctat atcatggtcg ctggaaaacg cgttgcggtt
    17701 gacgtcgcaa cgcttaagcg gcgcggcacc ggccgaggta ataccgtcaa gcttcatttg
    17761 aggttcgata aggtcgcgac ccggctcatt gaacgtctgc aagccaccct tggcggaacc
    17821 gtacctgacg gaatgacggt gttgctcacc atcaccgctc ccatccgttt gccatcgaaa
    17881 accgctgccg ctttggaaga caaaataaaa actcttctcg gccggaaatc gcctggttcg
    17941 gataagaaag acacgattca cgggaatagt ttccggattc gactcttgag aaacgacgct
    18001 gagcgagcgc caaaaatgat tgggttcgtg cacaattccg attccgacgc acttctactt
    18061 ttgaacatga cacgtgaact tcttgagcga atcggtgcga cggctggtag gcgggcgacg
    18121 aggcttgcgg gtgatcgatg gctcgttctg ataagccccg aaggagcctc aggcttacaa
    18181 gcataccgct acatctcttc gcagttgcga atggcggccg gctttcagaa gattctcatg
    18241 gcgtttggcg acgggcgcgt tgagatgtta aagggataac cccagttgcg cgcagtgcct
    18301 ctcagttcat cagtcagatg ccggttacaa gggagcggca gcgtcaggaa tgctggcggc
    18361 gattttcgat ttcgtgcaga tgctctttca gccggtccag aacctcgctc tttttttcat
    18421 tgcagatttc ccgctccact tcgtcgagcg caaaatggac gatatcgtga tggtggattt
    18481 cttttccggg aaggtaatcg ctgaattcga gccacactct gtgcagcagg tcgatttctt
    18541 tggttgtcaa tcgcggggcg tgagggccga gatagaaaat ctgttcccgg ccggaagggg
    18601 aaaagacctc gagcgagaca cgaggcttgg gttcggggat gcgttcccac gcgaggccga
    18661 tgtcgcgcgc ctgttcggtg acgctggtat tcgaggaact gcctgcgacg atggagctcg
    18721 actgaagatt ggccgcggtg cgcaagatgc cttcccagag gtcattcgcc gccgcgacgg
    18781 ccagtcgcac cggcttgcct tccttttcgg cgatagccag aactttggtg aagagcaact
    18841 gctcgatgtt ggtgaagagc tggtccgccg ccagagcgta ttcgcccgaa gcggcccgac
    18901 gcagcatgcg cacatgcagc accacaattt ccgcctcgcg ccgtttggcg cgccgtaaag
    18961 cggcttccag aggatacagc gcgtagtagt tgctcaatgg cacaagaatg ttgccgggcc
    19021 ggacgcccac attttccagg gtgagctcgc cttcctgggt gagattgaat ttatcgagct
    19081 cggcgtggga tttactttcc tttttggcga tcttttccga gatttcgaag accgcaaaga
    19141 aaataagcgt gaaagcgact cccgaaacgg tggcgacctg cttggtgaag agattgatga
    19201 agcacaaggc gaacaatgtc agcgtgatca gagccactcc cacaggaatt tccacgttgg
    19261 cgatttttag gttcaaaggc acgcgaaatt cacggccgcc cggctgcttg tagcgcagca
    19321 caagcacggc cagacctttc atcgcaaagc tccacatcac gccgaacgcg taagcctcgc
    19381 cgagcagaaa gatgtctccc cggctcagca cgattgtgaa gatctgtaag ccgacgacaa
    19441 gattcaggat tcgatgggag gtcccgaagc ggcggtgcgg atggcgaaac cagctgggca
    19501 gaatgccgtc ctcggaaacg cggttcaaga cgccgttgga gcccacaatc gccgtattaa
    19561 ccgctccagc cagcatcaaa actcccacca caacaacgaa aatgcggaac gcgattcgcg
    19621 cggtcaaagg cccaacgaga tgcatggcca gcccgctgat gggattgtcc gagaagttgc
    19681 cgcgcaccga gtccggaata atcatcactg cgaagaacgc cacgcccgcc gtgaaaatca
    19741 tgctgtagag aaagatcacc aacccggctt ttttcaggtt aggcagcttg ggatgttcga
    19801 tttcgcggta gacctgcgcc atcgtttctt caccgctcat ggccaaaacc gagtggccca
    19861 gaccgatcag aatgccgatc aagccaattg tgtacggcag cgaggtgtgg cgcagccaac
    19921 cgagggcgtc gctcgagtaa gtgatgttcg aaaggtgcgg ccagggtgga agatggacac
    19981 cacgcaccca gagcgtacag ccgcaccata caatcatcag aaccaccatc acggtggtca
    20041 cgtacatgat gcgcagcgct ttttcgctgg attcgggaat gcccttgatg ttttcccacc
    20101 agaaatagag cgtaactagg atggcaaacg cagcggcggt tgcgttcacc ggcagcacca
    20161 gattgaagtg cgcgtagcgc agcaattcgt tcaaaaggcc aaccagatac aaccctgccg
    20221 aaacgccgct gatcggaccc gtgaggatgt agtcgaacat caacgctgat acggaaaact
    20281 tcgccagcgt cgaacccatt ccttctttca ccacgcgata cacgccgccg cgcacgaaca
    20341 tcccgcagct ttcgtagtaa agctgctgaa ctgcgaaggc gaaaagcatg atggcgagca
    20401 cgaaccacgg agctgtcttg ccgatgaagt gttcggcaat tgcgcccgcg tagaatgcgg
    20461 acgatcccaa atcgttgagg accacggcgg ccgcccgcca atacgaaatg aaggccagca
    20521 tggccgtggt ggccacgagg acttttgccg ccggccgaac agcactagcg agagaggaat
    20581 cggtagtctt tgacgccatg aatagcccgt tacaactgta gcgggacggg tgtaagcacg
    20641 ccataaggaa ggcatgaaga tttcataaag acgatttcga aaacctccac cgtctcaatg
    20701 acatacggca tgccaaaatt tcggggcctc caggttgcac gatcgaacga caatctctac
    20761 gatgaagtga gtgaaagagt tcgtccctca ccggctgctt cgcaatcgcc acttgatgac
    20821 catcgcgtct gcgttcttgc ggaggcgctt cccgcggctg ccagcagccc aaccaaggct
    20881 ctttgacgtg gagccggaaa ctcaaatgcg cggccattgc tactggcacg cggaaccgcg
    20941 caaacatccc actctcctgc tggtgcacgg cctggaaggg tcgagcgatt cggcgtatat
    21001 ccttggctgc gccgaaaagg gatttgtggc gggcttcaat gtg
//
LOCUS       DATNDT010000159         4922 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4115082, whole genome shotgun sequence.
ACCESSION   DATNDT010000159 DATNDT010000000
VERSION     DATNDT010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4115082"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            537..956
                     /gene="ndk"
                     /locus_tag="VN788_05265"
     CDS             537..956
                     /gene="ndk"
                     /locus_tag="VN788_05265"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HXU19975.1"
                     /translation="MAVERTLAIIKPDAVGRAFVGEILSRIHAAKFQIVAIKSLRLTK
                     SEAEGFYAVHRGRPFFGELTEFMSSGKAVVMVLEAENAILKWRDTMGATDPAKAALGT
                     IRKELGTSIQYNCTHGSDAPDTAAFEIGYFFAGFDLI"
     gene            1185..2855
                     /locus_tag="VN788_05270"
     CDS             1185..2855
                     /locus_tag="VN788_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXU19976.1"
                     /translation="MAKPILLSVDDDSDVLRAIERDLRSKYGSEYRVIGTDSPEGALN
                     LLKQLQLRNDSVALLLADQRMPHMDGVAFLQEGMRIFPEAKRALLTAYADTNAAISAI
                     NEASINYFFMKPWDPPSEHLYPQLDDLLDDWRASFKPKFEGIRVLGTRWSPQSYAMRD
                     FLARNHVPYQWIDAELSANDPETKRLLEALGPEAVNLPIVLFPDGTKLLESVPADVAQ
                     KVGLRTRAQTNFYDLAIVGGGPAGLAAAVYGASEGLKTVMIEQEAPGGQAGMSSRIEN
                     YLGFPTGLSGGDLARRAVVQAQRFGVEILSPQEACGVRIEGPYRIVKLADGNEISCHA
                     LMIATGVQWRRLEAPGIARLQGAGVYYGGGSTEALSCKGEIVYVVGGANSAGQAAMNF
                     AKYAERVVIAVRGASLSSTMSQYLIDQIKETSNIQIWPHASVTEAHGDTHLEEISFLC
                     SDTNRIERVPATSMFIFIGALPRTDWLAGLVDRDDYGFILTGPDLIREGQRPKNWALD
                     RDPYLLETNIPGLFAVGDVRHASVKRVASGVGEGSVAVQFIHQYLSKV"
     gene            2856..4238
                     /locus_tag="VN788_05275"
     CDS             2856..4238
                     /locus_tag="VN788_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU19977.1"
                     /translation="MIEKSELLHVPAFADLPDDQIDWFLSQSEEIRLKVGDTPFHPGD
                     PADAMFVILDGQLQARGDLGGETVVIAVKPGTVAGVLPFSRMKQFPVGVQALTDARVL
                     RFPSSKFPDLIQKMPELTQRLVGLMSDRIRETTRREQQRDRLVSLGKLSAGLAHELNN
                     PASAAKRAASQLRDALRRIKDSSHELGRRDLTAPQKAEIEKLEASFVQSGDVPSDPLA
                     ISDLEDQIDSLLRSHGQNDLWQMAAELARRNVKPEALESLFATLDPQSARAALVRIAA
                     SVELATLLNEIESGTSRISDLVRAIKEYTFMDQAPLQNVDIVKSLETTLVIMNHKLKH
                     GVTVQRDYQKIPLLVNSFGSELNQVWTNLIDNAIGAMGGKGELRVRTRRDDKCVVIEI
                     ADNGPGIAPDILPHIFEPFFTTKGVGEGTGLGLDTVQRIVNKHRGDIQVSSKPGDTRF
                     QVWLPLSDAS"
     gene            complement(4307..>4922)
                     /locus_tag="VN788_05280"
     CDS             complement(4307..>4922)
                     /locus_tag="VN788_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19978.1"
                     /translation="NPNRMFIWAHSAGNVPLGTYIGHPDLYGPKGVGVKGVIFMSAAA
                     FDIAPLKPPPVGGSNPMSMLANAGKTCGVKGGAFSTEGALPGRAAGQPGGPPAAGGPG
                     GFGPPVDAATQLARSNLPELKKTSVKIMLANGDLDIGADPSVDGGVMPFNRMLHDELC
                     KEGPAHCPKLLLAKGESHMSMVFSIDTADKSVSGPILAWMKGIR"
BASE COUNT         1097 a         1378 c         1375 g         1072 t
ORIGIN      
        1 gaaaatcgat gagcgtgctg ctaccaccag tcagcccaca gagccaacca aagtagctgt
       61 ggccgcgccc tcgagccaag atgtggccca ccgagtcatt cccaacgcag ctcaaagcgc
      121 tctggacacc atccggggaa ccgtaagagt cagcgtcagg gcccatgtca attcctccgg
      181 cgacgtgtcg gaggttacct tggattcgcc aggaccaagc aagtatttcg caaggctcgc
      241 ccagcaagcg gcgcagcagt ggaaatttgc gcccaataga accacaagag attggatttt
      301 gcgttttgaa tttacaaccc cgggcgtcaa agctttcgcc gcgcccgccg ccccctaaac
      361 aatcgtccgc cacatttccg gattgccagt cgcggaactt cctgggcgaa aactggaagg
      421 ataaggcgct ggcaggcgcc agagttttcg cggtgcactt tttcctttgg agaccttaca
      481 atggtttgct ctcgcacctc cgcaagcact taaaatcgac ttgaggggaa tatcgaatgg
      541 cagtcgaacg cactctcgca atcatcaaac cggacgcggt cggccgcgcg ttcgtcggcg
      601 aaattctcag ccgcattcac gcggcgaaat ttcagattgt cgccatcaag tcgctgcggc
      661 ttaccaaatc tgaggcggaa ggcttctacg ccgtccatcg cggccggccg tttttcggcg
      721 agctcactga attcatgagt tctggaaaag ccgtggtgat ggttctcgag gcggaaaacg
      781 ctatcttgaa gtggcgcgac accatgggcg ccacggatcc ggctaaggcc gccctcggta
      841 cgattcgcaa ggaactcggt accagcattc agtacaactg cacgcacggc tccgacgccc
      901 cggataccgc cgcgttcgaa atcggttatt tttttgcagg ctttgatcta atttaatccg
      961 atcgatccca gtcgttctct gccgtccctg caagggtctg gattttctta aatttcaagc
     1021 gccatcaaga tccaccggtg acggctccgg attgtctgat ctgtcggcgc acgccgctgt
     1081 gaaaatctat atgcgaccaa accggagtcc tttcatccta taaggctgtt gtctaggcga
     1141 ttcaaacgtc tagaattgcc ctataacaaa aatgggaacc gatcgtggca aagccaattc
     1201 tgctgagcgt ggatgacgac tctgatgtct tgcgagcgat cgagcgcgac ttgcgctcga
     1261 agtacggctc tgaataccga gtgataggaa cggattctcc ggaaggcgcg ctgaatcttc
     1321 taaaacagtt acagctacgg aacgacagcg tggcgcttct tcttgccgac caacgcatgc
     1381 ctcacatgga tggtgtcgcg tttctacagg aaggaatgcg tattttcccc gaggcaaagc
     1441 gcgcattgct caccgcctat gccgacacaa acgccgcgat ctccgcgatc aacgaagcca
     1501 gcatcaacta ctttttcatg aagccatggg atcctccgtc tgagcatctg tacccgcaat
     1561 tggatgatct gctcgacgat tggcgagcgt cgttcaagcc caaattcgag ggaatccgcg
     1621 tgttgggcac acggtggtcg ccacagtcat atgcaatgcg tgactttctc gcgcgcaacc
     1681 atgttcccta tcagtggatt gatgccgaac tctcggccaa tgatccagag acaaagcgtc
     1741 ttttggaagc gctcggaccc gaggctgtga acctgccaat agtgctcttc ccggacggta
     1801 ccaaacttct cgaaagtgtg ccggcggatg tagcccaaaa agttggattg cggacccgag
     1861 cgcaaaccaa cttttacgac ctcgcaatcg tgggtggtgg accggcggga ttggctgctg
     1921 ccgtctatgg cgcatcggag ggactgaaga cggtgatgat cgagcaagag gcgcccggtg
     1981 ggcaggcggg aatgagctct cgcatcgaga attacctcgg atttccgaca gggcttagcg
     2041 gcggcgattt agcgcggcga gcggtggtgc aggcccaacg ttttggagtg gaaatcctct
     2101 cacctcaaga agcctgcggc gttcggatcg aagggcctta ccgcattgtc aaattggcgg
     2161 acgggaacga aatttcgtgc cacgcgttaa tgatcgcaac aggagtacag tggcggcgac
     2221 tcgaagcgcc cggcatcgcg cggttgcaag gcgcgggtgt ttattacgga ggcggttcta
     2281 cggaagcgct ctcctgcaag ggagaaatcg tttacgttgt gggcggcgcg aattctgctg
     2341 gtcaagccgc catgaatttt gcaaagtatg ccgagcgcgt ggtcattgcg gtgcgtggtg
     2401 cctctttatc gagcaccatg tctcagtatt taatcgacca aatcaaggaa acatcaaaca
     2461 tccagatttg gcctcacgcc agtgtgaccg aggcccacgg agatacgcat ctggaagaaa
     2521 tctcttttct ttgctccgat acgaacagga tagagcgcgt gccggcgact tccatgttca
     2581 tttttattgg cgcactgccg cgaactgact ggctcgcggg tctcgtcgac cgcgacgatt
     2641 acgggttcat cctgacaggc cccgatctca tccgtgaggg gcagcgtcca aagaactggg
     2701 cgctcgatag agacccatat ctccttgaga caaatattcc cggccttttc gctgtaggcg
     2761 acgtgcggca cgcttccgtg aagcgagtgg cttccggcgt tggcgagggg tcggtagccg
     2821 tccagttcat ccatcaatat ttgagcaaag tttaaatgat cgaaaaatcg gaactacttc
     2881 acgtgcccgc atttgccgac cttcctgacg atcagattga ctggtttctg agccagtcag
     2941 aagagatacg gctcaaggtg ggtgacacgc cattccatcc gggcgatccc gccgatgcaa
     3001 tgttcgtaat tctcgatggt caacttcagg cgagaggtga ccttggcggt gagacggtgg
     3061 taattgcggt caagcccggc acggtcgccg gcgtgctccc cttttcgaga atgaagcagt
     3121 ttccagtggg agtacaagca ctcacggacg cccgcgttct gcggtttccc tcttcgaagt
     3181 ttcccgatct tatacaaaag atgccggaat tgactcaacg cctcgtcggc ctgatgtcag
     3241 accgaattcg cgagaccacc cgccgtgaac aacaacgcga ccggttggtt tctttgggga
     3301 agctctcggc cggtctcgcg cacgaactca ataatcccgc ttccgccgcc aaacgcgctg
     3361 ccagccagtt gcgggacgcc cttaggcgaa tcaaagattc aagccatgag ctgggtaggc
     3421 gggatctgac tgctccccag aaagccgaaa ttgagaagtt agaggcatcg ttcgtgcaaa
     3481 gcggcgatgt tccatctgat ccgcttgcca tcagcgacct cgaggaccag atcgattcgc
     3541 ttttgcggag tcacgggcag aatgacttgt ggcagatggc ggccgaattg gcgcgccgga
     3601 atgtgaagcc ggaagcgctg gaatctctat tcgcaactct tgatccacag agtgcgcgag
     3661 cggcgttggt caggatcgcg gcgtcggtgg aactggcgac gttgctcaac gaaatcgaga
     3721 gtggcacatc gcgcatttcc gacttggtcc gtgcgatcaa ggaatacacg ttcatggatc
     3781 aagcgccact gcaaaatgtg gatatcgtga aaagtctgga aacgacgctt gtcatcatga
     3841 accataagct caaacatgga gtaaccgtgc agcgcgatta tcagaagatt cccctgctcg
     3901 taaattcatt tggaagcgag ttgaatcagg tttggaccaa tctgatcgat aacgccattg
     3961 gcgctatggg cggcaagggc gagcttcgag ttcgaacccg ccgtgacgac aagtgcgttg
     4021 taattgagat tgccgacaac ggtccgggaa tagcgcccga tatcttgccg catattttcg
     4081 agccattttt cactacgaag ggggtaggag agggtaccgg gctggggctt gacactgtcc
     4141 aacggatcgt gaacaaacac agaggagata tccaggttag ctcgaaaccc ggcgacacgc
     4201 gctttcaagt gtggctgccg ctgtcggatg cttcttgact gccgccgttg cccacgtgac
     4261 gctagtcact caactacgtg atttttcgat tggcaggctc tcgaagttac ctgatcccct
     4321 tcatccacgc gagtatcgga cccgaaacgc tcttatccgc agtatctatc gaaaatacca
     4381 tggacatgtg actctcaccc ttcgcaagca gcagcttcgg gcaatgagcc ggaccttcct
     4441 tgcagagttc gtcgtgcaac atccggttaa aaggcatcac gcctccatct acggacggat
     4501 ccgcgccgat gtccagatca ccgttggcaa gcatgatttt cacggaagtc ttctttagct
     4561 caggcaggtt tgatcgcgcc aattgcgtcg ctgcatccac aggggggcca aatcctccag
     4621 gacctccggc ggcgggtggc ccgcctggtt gaccagcggc acggcccggc agcgcgccct
     4681 cggtgctgaa ggctcctccc tttacgccac aagttttgcc tgcattggca agcatggaca
     4741 ttggattcga cccacccact ggcggcggtt tcagtggcgc tatatcgaac gccgccgcag
     4801 acatgaaaat cactcccttc acgcccactc ccttgggtcc gtagagatct ggatggccaa
     4861 tgtacgtccc gagcggcaca ttaccggccg aatgagccca gatgaacatc cgattcgggt
     4921 tg
//
LOCUS       DATNDT010000160         4213 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2627286, whole genome shotgun sequence.
ACCESSION   DATNDT010000160 DATNDT010000000
VERSION     DATNDT010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2627286"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VN788_05285"
     CDS             <1..625
                     /locus_tag="VN788_05285"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU19979.1"
                     /translation="TGKKIINPAFEPKANKTDFICPNANGARNWPGTSYDSATHILYV
                     PIAESCMDYTWVPNDAAQTAAGGLDIRFTMRLRPNTDGKMGRIEAVNLETKKVVWMDR
                     QRAPIASAMLATAGGLLFGGAQDRQFTAYDAETGKALWQVGLNATPSSSPVTYSANGK
                     QYVAVVSGGGGPLDAGGHSLAPEFDNPAGGTTLWIFKLPDAHQVAQQ"
     gene            832..2634
                     /locus_tag="VN788_05290"
     CDS             832..2634
                     /locus_tag="VN788_05290"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU19980.1"
                     /translation="MTFGAIEVRAQAQRSSSGAQGAPAPEPGNVLPPNHDAAFNATTA
                     RRAALLNNIAPVTDDMLRHPSDGDWLTWRRTYDSLGYSPLHQIDANNVGNLREAWAWT
                     LPTGRNEIAPLVHDGIIFIKSANTIQALNGRTGDLLWQYVRPLPASLHGGSSAIVRNL
                     AIYQDILYAPTADGHIVALNVKTGAPLWDQQVLGPKEIAHHLELDGGPIVAKGKVMMG
                     ASGCNTYPGGCFIVGLDAHSGKETWRFHTIARPGQPGGDSWNGAPVEQRFGASVWTSG
                     SYDPDLNLVYFGIGQTYDTGTLLEAHSENSDSNDGLYTDSTVALDPDTGKLVWYYQHM
                     NRDVWDLDWAFEQSLITLPIDGKPANLVVTGGKLMVFDAVDRASGKYEFSKDLGLQSL
                     VRAIDPKTGKKIINPALEPEANVTKHFCPHSGGGRNWPATSYDPETKILYVPLYESCQ
                     DFTWVPRDSAKTAEGGSDMHFVLHPRPDSDGKMGRIEAINLQTKKVVWAYRQRAAISS
                     SMLTTAGGLVFAGSRDRRFRAFNAATGKILWQNTLNASLSSTPVTYSIGGEQYVAIVA
                     GNGGPVAWPQLTPEIPNPVGGTTLWVFKLPAAGN"
     gene            complement(2713..3891)
                     /locus_tag="VN788_05295"
     CDS             complement(2713..3891)
                     /locus_tag="VN788_05295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU19981.1"
                     /translation="MRILKVVQAYYPFQEKGGPVVKVRALARGLAQRGHPVTVLTADL
                     GLRHFRDAGGNFEACKWGWRSEQDGVEVIYLSTLCRYRAVTLNPHIIGFCRESLNRFD
                     VAQIYGLYDWLGPTVGFFCRRRGVPYVVEPMGMFRPIVRSLALKRLYHGVFGNSLIAG
                     ARFLIATSEQERQEMIGGGVATTRIVVRRNGIDSPEKLPNRGEFRRQWNIPENARVVL
                     FLGRVVSKKSPDLLIDAFSRWHAKSGNENSVLVIAGPAEGDGFLPRLKMMAQQLNLSQ
                     SVRFVGPLYEAAKWQAYRDADVFVLPSQNENFGNTAAEAAACATPVIVTDQCGVAPFV
                     GRAGLVVRHDPRAIELALAQLLEDSATRRRCQDGCAEMARSLSWDAPLLENEQLLERC
                     "
     gene            complement(3957..>4213)
                     /locus_tag="VN788_05300"
     CDS             complement(3957..>4213)
                     /locus_tag="VN788_05300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018778364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU19982.1"
                     /translation="ASGGTTDVIEDGVNGLLVPPNDPERLAQALDRLLRDESLCAELG
                     RRGAEIVRQKFQFSTFQAELERLVTDCVLVGENLASANRG"
BASE COUNT          949 a         1239 c         1199 g          826 t
ORIGIN      
        1 aacgggaaag aaaatcatca atcccgcgtt cgagccaaaa gccaacaaga ctgatttcat
       61 ctgccccaac gcgaatggcg cgagaaactg gcccgggacc tcgtacgact ctgccactca
      121 cattctgtat gtaccaatag ccgaatcttg catggactat acctgggtcc ccaacgacgc
      181 cgcgcagaca gcagcgggcg gcctcgatat tcgcttcacc atgcgcctgc gtccaaatac
      241 cgacggcaag atggggcgca tcgaagccgt gaatcttgag acaaaaaaag tagtatggat
      301 ggatcgtcag cgtgctccta tagccagtgc gatgttagcg acggcgggcg gccttctgtt
      361 cggcggcgcg caggaccggc agttcaccgc ttacgacgcg gaaactggca aagcattatg
      421 gcaagttgga ttaaacgcaa cgccgagctc ttctccggtg acttacagcg ccaatggcaa
      481 gcaatatgtc gccgtggttt ccggcggcgg aggaccgctc gacgccggtg ggcacagcct
      541 agcgccagag tttgataatc cggcgggtgg gacaacgctc tggatattca agctgcccga
      601 cgcacaccag gtcgcgcagc aataggacta gaggcgagac agagacccgt cagaaaagtc
      661 agataaggaa tgccaagccc atcaaaatct ctcagtagga acgaaaacgc acttcggcac
      721 gccctctcaa tcctcggccc aaaatgccgg ccatcatcta gcaacaagtc cgcctgtaac
      781 actacagttc agagatggtt ttcgcgaggc gcgctttttg ttttagcggc catgacgttt
      841 ggtgccattg aagttcgcgc gcaggcccag agatcttcat cgggagcgca gggagcgccg
      901 gcaccagagc cggggaatgt tcttccgccg aatcacgatg ctgcattcaa tgcgaccaca
      961 gcgcggcggg cagccctgct caacaatatc gcgccggtga cagacgatat gctccggcat
     1021 ccatcagatg gagattggct gacttggcgg cgcacttacg acagtttggg ctatagccct
     1081 cttcatcaga tcgacgccaa caatgtaggc aatctgcgcg aggcgtgggc ttggacgttg
     1141 ccaacgggtc ggaacgaaat cgcaccgctc gttcatgacg ggattatctt catcaagagc
     1201 gccaacacga ttcaggcgct gaatggcagg acaggcgatc tgctctggca atatgtccga
     1261 cctctgcccg cgtctctcca tggcgggagc tcggcgattg taagaaacct tgccatttat
     1321 caggacattc tctacgcgcc caccgccgat ggccatatcg tcgccctgaa tgtgaagact
     1381 ggcgcgccct tgtgggatca gcaggtccta ggcccgaaag agattgcaca ccatctggaa
     1441 ctagacggcg gtccgattgt cgccaaaggc aaagtgatga tgggcgccag cggctgcaac
     1501 acgtatccgg ggggttgctt catcgtcggg ttggatgccc acagcggcaa ggaaacttgg
     1561 cgattccata ccatcgcgcg ccccggccaa cccggtggcg acagttggaa tggcgcgccc
     1621 gtggaacagc gctttggcgc ctcggtgtgg acatcgggca gttacgaccc cgatctcaac
     1681 ctcgtttatt ttggtattgg ccaaacttat gacaccggga cattgctgga ggctcattcc
     1741 gagaacagtg attcgaacga cggcctctac accgactcca ccgtagcgct cgatcctgat
     1801 accggcaagc tcgtctggta ttaccagcat atgaaccgcg atgtctggga tctggattgg
     1861 gcgttcgagc aatcgctgat cacgctgccg attgatggca aaccggcaaa tctcgttgtc
     1921 accggcggca agctcatggt attcgatgca gtcgatcggg cgagcggtaa atatgaattc
     1981 tccaaggatc tcggcctgca aagtctggta cgtgcgatcg acccgaaaac gggaaagaag
     2041 atcatcaacc ctgcgctcga gccggaagcc aacgtgacca agcacttctg cccgcattca
     2101 ggcggcgggc gcaattggcc tgcaacatct tacgatcccg agaccaaaat tctgtacgtt
     2161 ccgctgtacg aatcctgtca ggactttacg tgggtgccgc gcgattccgc caaaacagct
     2221 gaaggcggta gcgacatgca tttcgtcctc catccccggc cggatagcga cggcaaaatg
     2281 ggccgcatcg aagcgataaa ccttcagact aagaaagtgg tgtgggccta tcgtcagcgc
     2341 gcggccatct caagttcaat gctgacgact gcgggtggac tcgttttcgc cggctcgcgt
     2401 gaccggcgct tccgtgcttt caacgcggcg acgggcaaga tattgtggca aaacacatta
     2461 aatgcctctc tcagttccac cccggtcacg tatagcatcg gcggcgaaca atacgtggct
     2521 atcgtcgccg gcaatggcgg accggtcgct tggccccagc tgacacccga gatccctaat
     2581 cccgttggtg gaactacgct ctgggttttc aagctgcctg ctgctgggaa ttgaagttgc
     2641 ccacgcgggt gttgcaacgg ccgctcgtga tcgcctaccg gggtgcaggc gctacttcgt
     2701 gttcgcctag cgctaacatc gttcaaggag ctgctcgttt tcgagaagag gagcgtccca
     2761 agagagagaa cgagccattt cggcgcaacc atcttggcag cgacggcgag tagcagagtc
     2821 ctcgagtagt tgcgcgagtg ctagctcgat cgcgcgcgga tcgtggcgca ccacgaggcc
     2881 cgccctcccg acgaatggcg ccacgccgca ctggtccgtg acaatcactg gcgtggcgca
     2941 agcagcggct tcggccgcgg tgttgccaaa attctcgttt tgagagggaa gcacgaaaac
     3001 atcagcatcg cgataagcct gccatttcgc agcttcgtaa agcggcccaa cgaaccggac
     3061 gctctggctc aaattcagct gttgagccat catcttaagt cgaggaagga agccgtcgcc
     3121 ttcagcaggg ccggctatca ccaacacact attttcgttg ccggactttg catgccatcg
     3181 cgaaaacgca tcgatcagca agtctggact tttttttgac accacccgtc cgaggaagag
     3241 cacgacacgc gcattttcgg gaatgttcca ctggcgacga aactcgccgc ggttggggag
     3301 tttctcaggc gagtcgatcc cgttgcgacg gacgacgatc cgggtagtgg cgacgcctcc
     3361 cccgatcatc tcttgacgct cttgctcgga cgtggcgatc aaaaagcgcg cgccagcaat
     3421 gagggaattg ccgaacacgc catggtaaag ccgcttcagt gccaagctgc gaacaatcgg
     3481 acggaacatg cccatcggct cgacgacgta cgggacacct cgtcgccggc agaagaaacc
     3541 cacagttggc cccagccagt catacaaccc gtaaatttgg gcgacgtcaa agcgattcag
     3601 ggactcgcgg cagaatccga ttatgtgcgg attcagcgtg acagcacggt accggcagag
     3661 ggtagataga tagatgacct cgacgccatc ttgctccgag cgccatcccc acttgcatgc
     3721 ttcgaaattg ccgcccgcgt ctcgaaaatg gcgaagccca aggtcagcgg tgaggaccgt
     3781 cacggggtgt cctctttgag ccaaaccccg cgcgagggcg cgaactttta ctacgggtcc
     3841 acccttttcc tgaaaaggat agtaggcctg cacgaccttc aggatgcgca tggcggaatc
     3901 gctttcacgc taccaaaaac ctcgtgggtg gggttgtaat aggcgctccc agaatactat
     3961 ccgcgatttg ctgaagccag attctcgcct acgaggacgc aatcggtcac caagcgttcg
     4021 agttcagctt gaaaagtgct gaactgaaat ttttggcgaa cgatttccgc gccccgccgg
     4081 cctaactccg cgcaaagtga ctcatctcgc agcagacggt caagcgcctg agcgaggcgc
     4141 tccggatcgt tgggtggcac aagcaggccg ttgactccgt cctcaatcac gtccgtagtt
     4201 cctccgctcg ccg
//
LOCUS       DATNDT010000161         6334 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1441462, whole genome shotgun sequence.
ACCESSION   DATNDT010000161 DATNDT010000000
VERSION     DATNDT010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6334)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6334)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6334
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1441462"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3585)
                     /locus_tag="VN788_05305"
     CDS             complement(<1..3585)
                     /locus_tag="VN788_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU19983.1"
                     /translation="MSISGYVTYTDTVSSLMFVQDSTGGIRVQRPLPFAAVAAGQAVE
                     VTGAAGAGLPNPAVIRAHVKALPNHWQPEPIPIGARDFERNDLQYRRVEVRGIVRATN
                     IERTGRLGLTIHVNGIDIQAHVLEFLLNDYNALIDSTIRVPGVLDTSFDASGRPAEVR
                     LWVNTADEIQVEHKAENSAFLPIRSARNLLAMKPGHWPDNLVRVHGVVSAKGSGSELV
                     VRDDTGQLPMQLEPSSSQIAGAGLDAYGFLAKANGRTILEHAFLRKPGGINRSGNSTD
                     SLPVLTTVSEIHALSAVEANRQYPIHIQGAVTYFNPEDEILFVQDATGGIFVYSHGLN
                     VPGLKAGKLADVRGVSGPGDFAPMVMAPQIHVLGDTSLPPIPSVTTENIFSGKEDSVW
                     VQVDGIVHALGMREGMAMISLKSDLRDFQAVVQGVTKLPNSLINKKIRLKGVCGSRFN
                     TRRQLLGILVYVPGPGYLEIEPDTAMAEKVASTPIADLMEFSPTEIPGHQALITGSVT
                     LASPQGPTYIQDSSGGILIRNHKEAALQIGDIVEVSGFPYTGQFIPVMHDAVIKKLAS
                     GHSPEPIRVTADQILEDGDDAELVQLEAIAQNQVSTPTGEVLLLQAGSTLFQARMPQE
                     GELLNIRPGSLLRLTGICSIQVENSDDTAIPEAFTLLLRSPADIVILKDAPWWTIQRA
                     AGLVALLGSLFGLAFGCVVVLRRRVKQQTAVIRDKLAQVETLKEAAEGANRAKSEFLA
                     AMSHEIRTPMNGVIGMTSLLLDTPLTGEQQDYLDTIRTSGDSLLTIINDILDFSKIEA
                     GKLDFEKIDFDLRCLVEETAELVSELAERKALELQVVVDDDLPAGLIGDPGRLRQILL
                     NLLSNAVKFTERGSVTVRARKQGQSANGIFIHFAVMDTGIGITPAAQARLFRSFSQAD
                     SSTTRKYGGTGLGLVISKRLAEMMGGGIGVESIPGEGSTFWFTVWLPESQAIHLTPVP
                     WDSMPHQRVLVVDDNATNRQIARQHLNYAGLIATEAASGPEALMHLLSAAQGEQPFAL
                     AILDLHMPVMDGLMLARAIRSQEAGQRLPLLLLASHRDADLVAEAQSIGLSSYLLKPV
                     RRAHFLSAVCRALGRMHSERDRPLPVQIEPQAVPAQSRRRILVAEDTPTNQKVAALLL
                     DRLGYHAEVVANGLEAIEAIKMAPYDLILMDCNMPEMDGFAATQAIRQM"
     gene            complement(3859..5088)
                     /locus_tag="VN788_05310"
     CDS             complement(3859..5088)
                     /locus_tag="VN788_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXU19984.1"
                     /translation="MSPSSPVEEPQTLAREIEDLENRYLLQNYARYPLTLHRGRGCYV
                     YDTAGKRYLDLISGIGVNALGYAHPRIVKTIREQAGLLLHTSNLYYHEYQGRLAERLA
                     KISGLQRTFFCNSGAEGIEGALKMARAHGHKISPEKYEIVSLDNSFHGRTLGALSITG
                     QPKYRKDFEPLLPGPKFVPINDAAALEAAVNERTAGIFIEWIQGEGGINPMTDEYARK
                     ARELADKYDALLVFDEIQCGVGRPGTHFAYQLANPVVLPDVMVAAKPLACGLPLGVIV
                     ANERAASAIGPGMHGSTYGGGPVACRVALEFLDILEELLPSIRSTGEYFRAQLKDLGK
                     RYGFIKEVRGKGLMIGLELAIPGKQIVRDAIEEGVLINCTHDVVLRFLPPYIITESQV
                     DQAVRTLNKLFRKAKAT"
     gene            5128..>6334
                     /locus_tag="VN788_05315"
     CDS             5128..>6334
                     /locus_tag="VN788_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU19985.1"
                     /translation="MRRRVASYTEPDSAMRTRVSVFRLAGIVLLLLLFYTIAGGRLPF
                     EHQLIGFTRYDRAIQTGAVHASSRDLDHSLRLSLTILEFLLLLLAVAGAFGKTRIERW
                     LNLRVFSLSVTVASACTLFVFLSRLGVEAEWYPLAVLMTNPATLPIFGHRLLLVWPAD
                     ALKYLVPRLSYLQCYYATQIVAIFLAVITIGEWSAIFIGGRLKFIGQLLLVAMLGPTF
                     YYFTFYDIAMVFFFAWSLLLLYRGKYALFVLVLGLGTLNHENTLLLVPLAAFVLWREK
                     PPKIAMGVPCAGLGAYILARLAMQWAMPMHTLFYFRVWTNILELAHPSRGLVQSALSL
                     VFWWACAGIALPHAEPFLQRAAILFPLLLIVTILFGQFHEARQFDAFIPVAIALILSL
                     VRKVLANYMR"
BASE COUNT         1327 a         1849 c         1706 g         1452 t
ORIGIN      
        1 catttgacga atggcttgcg ttgcggcaaa accgtccatt tcgggcatgt tgcagtccat
       61 caggatgagg tcgtaaggcg ccattttgat ggcttcaatg gcctcgagcc catttgccac
      121 tacctcggcg tggtagccaa ggcgatccag cagcagcgcc gccaccttct ggttcgtcgg
      181 agtgtcttcc gcaaccaaga tccgtctacg gctctgggca ggtacggcct gtggctctat
      241 ctggaccggc aacggcctat cgcgctccga atgcattcgg ccaagcgcgc ggcagacggc
      301 cgataggaaa tgcgcccggc gcaccggttt gagaagataa ctgctcaagc caatgctttg
      361 cgcttccgcg acgagatccg catcccggtg agacgctagc agcagcaaag gcaggcgttg
      421 cccagcctcc tgggaccgga tagcgcgcgc cagcatcaac ccgtccataa caggcatgtg
      481 gagatccaga attgccagag cgaatggctg ttccccctga gccgccgata acaggtgcat
      541 cagcgcttcc ggaccgctgg ctgcctcggt agcgattaaa ccggcataat tgagatgttg
      601 ccgcgcgatc tggcgatttg tggcgttatc atccacgaca agaactcgct ggtgaggcat
      661 cgaatcccag ggaaccggcg tgaggtgaat cgcttgactc tcgggaagcc acaccgtgaa
      721 ccaaaatgtg ctgccttcgc cgggtatgct ttccacgcct atcccgccac ccatcatttc
      781 cgccaaacgc ttcgatatga ctaaaccaag cccggttcca ccgtatttgc gagtggtaga
      841 actgtctgcc tgagaaaaac tacgaaagag acgtgcttga gccgccggtg tgattccgat
      901 acccgtgtcc ataacggcaa aatggatgaa gatgccgttg gcggattgcc cctgtttgcg
      961 ggcccgcacc gtaaccgagc cgcgttccgt aaacttgacc gcatttgaaa gtaagttgag
     1021 aaggatctga cgaaggcggc cgggatcgcc gatcagaccg gctggaagat cgtcgtccac
     1081 cacgacctga agttccagcg cctttcgttc cgcgagctcc gagacaagct cggctgtttc
     1141 ctcgaccaga caacgcagat cgaaatcgat tttctcgaag tccagctttc ccgcctcgat
     1201 tttcgaaaaa tctaggatgt cgttgatgat cgtcagaagc gaatcccccg acgtccggat
     1261 cgtatcgagg taatcttgct gctctccggt caagggagta tcgagtaaaa gcgatgtcat
     1321 cccgatcaca ccgttcatgg gtgtgcgaat ctcatggctc atcgcggcta ggaattcgct
     1381 cttggcgcga ttcgcgcctt cggctgcttc cttcagcgtc tccacttgcg cgagcttgtc
     1441 ccgaataacg gccgtttgtt gcttgacgcg ccgccgcaga acgacgacac acccgaacgc
     1501 gagtccgaag aggctgccca gtagggccac caggccagcc gcgcgttgaa tcgtccacca
     1561 tggggcgtcc ttaaggatca ctatatccgc aggcgatcgg agcaggaggg taaacgcctc
     1621 cggtatagcg gtatcgtccg aattctcgac ctggatggag cagatgcccg taagccgcag
     1681 caacgaaccc ggccgtatgt tcaacagttc gccttcctgt ggcatcctcg cctggaagag
     1741 cgtgctgccg gcttgcagca ggagaacttc cccggttgga gtactgacct gattttgcgc
     1801 gatagcttcg agttgaacta actccgcgtc gtcgccatct tccaggatct gatcggctgt
     1861 gacgcggatc ggttccggag aatggcctga ggcgagcttc ttaattaccg catcgtgcat
     1921 caccgggata aattggccgg tatacggaaa gcctgaaacc tccacgatat ctccaatctg
     1981 gagagccgct tctttgtggt tgcgaatcaa aatgccacca cttgagtcct gaatgtaagt
     2041 agggccttgt ggactcgcca gcgtaacaga ccctgtaatc agggcttggt gtcccggaat
     2101 ctcagttgga gaaaactcca tcagatccgc aatcggcgta gacgcgacct tctcagccat
     2161 ggccgtatcc ggttcaattt ccagataacc gggaccgggg acatacacga gaattcccaa
     2221 caattggcgt cgcgtattga agcggctgcc gcaaacgcct ttcagacgaa tctttttatt
     2281 gatgaggctg ttgggcagtt tagtgactcc ttggacgacc gcctgaaaat cacgcaagtc
     2341 cgatttcagg ctgatcattg ccatgccctc ccgcatacca agcgcatgta caatcccgtc
     2401 aacttgcacc caaacgctat cttctttgcc cgaaaagata ttttcggtag ttaccgaagg
     2461 aattggaggc aagctagtgt cgcccaagac gtgtatttga ggcgccatta ccataggcgc
     2521 aaaatcaccg ggaccactta cacccctaac atcagcgagc ttgccagctt ttaagccagg
     2581 cacatttaac ccgtgggagt aaacaaaaat gcccccagtg gcgtcttgaa caaacagaat
     2641 ctcatcttcc ggattgaagt aagtgacggc gccttggatg tgaatcggat actgacgatt
     2701 cgcttccact gccgatagag catggatttc agagacagtg gttaggaccg gcaaggaatc
     2761 cgtgctgttc cccgaccggt tgatcccacc cggctttctc aggaaggcat gctcaagaat
     2821 cgtgcggccg ttcgcttttg ccagaaatcc ataagcatcg aggcccgctc cggcgatctg
     2881 cgaagacgat ggctcaagct gcatgggcaa ctgaccggta tcgtcccgca caacaagctc
     2941 cgaaccggat cccttggctg aaaccactcc atggacgcga accagattgt ccggccaatg
     3001 tcccggtttc atggccagta gatttctcgc ggatcgtatt ggaaggaaag cggagttctc
     3061 ggccttgtgc tcgacctgaa tctcatccgc tgtgttgacc cacaagcgta cttccgcggg
     3121 cctaccgctc gcgtcgaaac tcgtatcgag gacccccgga acccgaatcg tggaatcgat
     3181 cagcgcgttg taatcgttca agagaaattc caggacgtgc gcttggatat caattccatt
     3241 cacgtgaatg gtcagaccga gccgcccggt cctctcgatg ttcgtggcac gcacgatccc
     3301 ccgcacctcc acgcggcgat attgcagatc attcctttcg aagtcacgcg ccccgattgg
     3361 tatcggttcc ggctgccagt gattgggtaa cgctttcacg tgcgctcgga tgaccgccgg
     3421 attgggcaga cccgctccgg ccgcgccagt aacctccacg gcctggccgg ccgcgaccgc
     3481 agcaaaaggc aaagggcgct gcacgcgaat gccgccggtc gaatcctgaa caaacatcag
     3541 ggacgaaacg gtatcggtat acgtaacata cccggagata ctcacgggga tatgcgctgc
     3601 cgctgcccta acggggagat tgcgtaactg cgcggccgtg gttacgcgct tggccaacgg
     3661 tttatggagc gcggacccgc atcccgcaga aaaaacgaag gaagccgtga gataggcaca
     3721 gacaagcttc caatagccgt tatttccatc cccccacaac cccacatccg ttatatcgac
     3781 attaatgtgg tttttctgta tagggttagt atgattccgt tagataggtc aggccaccgg
     3841 cacgcgacgg aaatcctgtc aggtcgcttt cgctttacgg aacagtttgt tgagcgttcg
     3901 gaccgcctga tccacctggc tttccgtaat gatatacggt ggcagaaaac gaagcacgac
     3961 gtcatgcgtg caattgatca acacaccctc ttcaatagcg tcacgcacga tctgtttgcc
     4021 aggaatagct agttccaaac cgatcattag tcccttgccg cgcacttcct ttatgaatcc
     4081 atacctcttt ccgagatctt ttaactgcgc ccggaaatac tctccggtac tgcggatcga
     4141 aggtagcagc tcttccaaaa tatccagaaa ttcgagcgcg acccggcacg ctaccggtcc
     4201 tccgccatac gtcgatccat gcattcccgg gccaatcgcg gaagctgccc gctcattcgc
     4261 gacaatcacg ccgagcggca gcccgcacgc gagcggtttg gcggcgacca tcacgtccgg
     4321 cagaacaacc ggatttgcca gctgataggc gaaatgcgtt cccggccggc ccaccccgca
     4381 ttggatttca tcgaacacga gcagcgcgtc atacttgtcc gcgagttccc gagcctttcg
     4441 cgcatattcg tcggtcatcg gattgattcc gccctcgcct tgaatccatt cgatgaagat
     4501 ccccgccgtc cgctcgttta ccgccgcttc caaggcagca gcatcgttaa tcggcacgaa
     4561 cttcgggccg ggcagcagcg gttcgaaatc cttacgatac ttcggttgcc ccgtgatcga
     4621 aagcgctccg agcgtccgcc cgtgaaacga gttgtcgagc gagacgatct cgtatttttc
     4681 cgggctgatt ttatggccgt gggcgcgcgc catcttcaac gcgccttcga tgccctccgc
     4741 gcccgaattg cagaagaatg tgcgttggag gccgctgatc tttgcgagcc gctccgccag
     4801 acgcccctgg tactcgtgat aatagagatt cgacgtatgc aatagcaggc cggcctgttc
     4861 gcgaatcgtt tttacaatgc gcggatgggc atacccaagc gcgttcacgc cgattcccga
     4921 aatcagatcg agataacgct tgcctgccgt atcgtacacg tagcaaccgc ggccgcgatg
     4981 tagcgtgagc ggataacgcg cgtaattttg aagcagatac cggttctcaa gatcttcaat
     5041 ctcccgcgcg agggtctgcg gttcttccac tgggctgctt ggggacataa ctccataata
     5101 gggcacatgt tactaacgct tggagtgatg aggcggcgtg ttgcaagtta tactgaaccc
     5161 gactcggcca tgcgtacgag ggtttccgtt ttccgattag ccggcatcgt tctcctgctg
     5221 ctcctgttct acacgatcgc cggcggccgc cttccattcg agcaccagtt gatcggcttc
     5281 acgagatacg atcgcgcgat ccagactggc gcggttcatg caagctcgcg ggacttggac
     5341 cactcacttc gcttgagtct cactatcctg gaattcctgc tgctcttgct tgcagttgcg
     5401 ggcgcattcg gaaaaacgcg aatcgaaaga tggttgaacc ttcgcgtctt ctcactctcc
     5461 gtgacggttg caagcgcgtg cacgttgttt gtctttctgt cgcggctagg agtggaagcg
     5521 gagtggtatc ccctcgccgt gttgatgacg aaccccgcta cgcttccgat ctttgggcac
     5581 cggctattgc ttgtgtggcc ggcggatgcg ttgaaatacc tggttccgcg actctcgtat
     5641 ttgcagtgct actatgccac gcaaatcgtc gctatatttt tagcggtcat cacgattggc
     5701 gaatggtcgg ccatttttat cggaggacgc ctgaagttta ttgggcaact cctcttggtc
     5761 gccatgctgg ggccgacttt ctattacttc accttttacg acatcgcaat ggtgtttttc
     5821 ttcgcctgga gcctgctgct tctatatcga ggcaaatatg ctctttttgt gctggtgctg
     5881 ggtctcggta cgctcaatca cgaaaatacg ctgcttctgg ttccattggc ggcatttgtc
     5941 ctttggcggg aaaagccacc gaaaatcgcg atgggcgttc cttgtgcggg tcttggcgcc
     6001 tacattctgg cccggcttgc catgcagtgg gcaatgccga tgcacacgtt attttacttt
     6061 cgggtctgga cgaacattct cgagctggcg cacccatcgc gaggcttggt tcaatccgcg
     6121 ctgtcgctag ttttctggtg ggcctgcgcg ggcatcgcgc tgcctcacgc cgaaccgttc
     6181 ttgcagcgcg cggcgattct gtttccgcta ttactaatag tgacgatcct gttcggccaa
     6241 ttccacgaag cccggcaatt cgatgcgttt attccggtgg ccatcgcttt gatattgtcg
     6301 cttgtgcgca aggttcttgc gaattatatg cgtg
//
LOCUS       DATNDT010000162        13829 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3232208, whole genome shotgun sequence.
ACCESSION   DATNDT010000162 DATNDT010000000
VERSION     DATNDT010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13829)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13829)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13829
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3232208"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            220..501
                     /locus_tag="VN788_05320"
                     /pseudo
     CDS             220..501
                     /locus_tag="VN788_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(620..1402)
                     /gene="cobA"
                     /locus_tag="VN788_05325"
     CDS             complement(620..1402)
                     /gene="cobA"
                     /locus_tag="VN788_05325"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HXU19986.1"
                     /translation="MSANVYLVGAGPGDPELLTCKALRVLQSADVVLHDDLVSADVLR
                     MIRPGALKQNVGKRCGQKHITQEEIHMRMIDTARKGQTVVRLKGGDPLLFGRAGEEMD
                     ALRRAGIDFEVVPGVTAAFAAAASAKVPLTDRRAASRVIFLTAHSCGPDTLPDFGSGI
                     SSGATLAIYMPGQDYAHLQERLLRSGVDPMTPCLVVSRAMLPDESFCAAELGGLALAP
                     RMASPTILLVGPLTTSLQPTDSATKSASWLGEAAARPWRCLL"
     gene            complement(1399..1977)
                     /locus_tag="VN788_05330"
     CDS             complement(1399..1977)
                     /locus_tag="VN788_05330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HXU19987.1"
                     /translation="MALFPLFVRLENRPCLVVGAGAVGEAKTESLVQAGAAVWVVAPC
                     ATPRIKEWAASGGIAWLPRKFSPADLEGKFLVVAATSSVAVNDFVFQEARSRDVLCNV
                     VDDPPRCDFFYPAVLRRGDLQIAVSTNGKSPALAQRLRERFAREFGPEYAESVRKLGA
                     ARERLFQLRIAPARRREMLLRMARQLPLPGEK"
     gene            complement(1983..2678)
                     /locus_tag="VN788_05335"
     CDS             complement(1983..2678)
                     /locus_tag="VN788_05335"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006284387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HXU19988.1"
                     /translation="MISMKDEVLGQQAEAEVWSVGKLLRWAFDRFHPRLAVASSFGAE
                     GVVLIDIAARIQPGLRVFTLDTDFLFAETRDLMARIESKYGIDVERTRPALTPEEQAV
                     LHGQALWSREPNLCCQIRKVNPLKAKLAELDAWVTGIRRDQTPARASAKKLDWDAAFG
                     LVKLNPLADWTSAQIWDYIRANGVPYNALHDRNYPSIGCTHCTQPVQPGEDPRAGRWA
                     GFQKTECGLHAKE"
     gene            complement(2847..3053)
                     /locus_tag="VN788_05340"
                     /pseudo
     CDS             complement(2847..3053)
                     /locus_tag="VN788_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708480.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
     gene            complement(3403..5100)
                     /locus_tag="VN788_05345"
     CDS             complement(3403..5100)
                     /locus_tag="VN788_05345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase"
                     /protein_id="HXU19989.1"
                     /translation="MEARETKAQKAERLKKSLNPWEGLAEIRCFAREGYESIPAEWLG
                     TYFRWWGIYTQGDGMGAVGGSGGEGRAVRFFMMRIRIPNGFLSSRQLRTIADLIDQFG
                     LGFADITVRQNIQLHWITIEVLPEVLDSLARCGLSSLGACGDVVRNITGCPLAGVDAQ
                     EICDASPLVEQASRLLAGNSEFYNLPRKFKISITGCRDWCTYPEINDIGLTAVTRKRH
                     GGAETGFSLRIGGGLSSKPHLAVRLNAFIRQDQVLPVVQGVAEVFRGADVLRESRERA
                     RLKFLFLHHGWTAEQFQEELERRLGFRLDPAEPEEAPTSIYRDHIGIHAQKQPDLFYV
                     GANVLRGRILPGQMRRAADLAEQFASGELRATIMQNLLLVGVPKKYTAFVADELAAAG
                     LRVDASPFRRGVIACTGTELCKLAITETKAFSLWLVEDLEERFPGFDQHLKLHVTGCP
                     NSCGQHWIADIGIEGKKLRVNGEMVDAYYFCVGGAVGQLASIARPVGYRCAATEVPAA
                     IERLLRSYLERRRNEENLQQFFTRYTDQELRSFLAGINVEAVPRNVAPGHAPHEVQG"
     gene            complement(5481..6575)
                     /gene="asd"
                     /locus_tag="VN788_05350"
     CDS             complement(5481..6575)
                     /gene="asd"
                     /locus_tag="VN788_05350"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXU19990.1"
                     /translation="MKQYRVGVLGATGMVGQQIIHRLRRHPWFRVTALAASERSAGKN
                     YADVARWRIGEQLPLEVAGLTVQPLEANLDCDFCLSALDSSVAGPAELDFARAGYPVI
                     SNSRNHRMEANVPLLVPEVNPEHCALVAVQRRQRGYTSGFIATNPNCSAAGLVLALKP
                     IDDAFGLEALSVVTMQALSGAGFPGVPSLDAMDNVIPFISGEEEKMESEPRKILGRLL
                     NGAIELADIRISAQCHRVPVIDGHMEAVSLKLRRKASLADVAAALSEFRGEPQRMGLP
                     SAPARPIEVREEPDRPQTRFDRDAGEGMAVVVGRIRACHVLDIRLVLLSHNLGRGAAG
                     AAILNAELLAENGYIVPRDPSHAKHSVAHL"
     gene            complement(6599..7783)
                     /gene="thrC"
                     /locus_tag="VN788_05355"
     CDS             complement(6599..7783)
                     /gene="thrC"
                     /locus_tag="VN788_05355"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXU19991.1"
                     /translation="MTYDFDRVEPELLYRKWAQRKLSNEPADRSGVWRFRELLPFLDP
                     GLAIISLGEGSTPLVEALRAGEWAGGVGLTIKHQGNNPTGSFKDLGMTACITQAASQK
                     VRVVACASTGNTSSSMAAYAARAGMKAMLVVPVGGISSAKLAQAMDFGACVVEVGETF
                     DKAFQLLNAIAPEMGLYLVNSINPFRIEGQKTIVAELLEQRGWRPPEYIVVPGGNLGN
                     TAAIGKGLRELRFLSLIEKVPRVVVVQAAGANPLYKMLASGSTELVPVADPRTDATAI
                     RIGNPANWRKARQVLQWTNGLCESVTDEEISEAKAVLAADGVGCEPASAATAAGLRKL
                     CRAGQIEKGAEVVAILTGNQLKDTDYILKRRSQGNASGWREQAEPTQSGLRRVLEAHS
                     IP"
     gene            complement(7898..9313)
                     /locus_tag="VN788_05360"
     CDS             complement(7898..9313)
                     /locus_tag="VN788_05360"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXU19992.1"
                     /translation="MIRATGEHQLLVMKFGGTSVGDAECISRVAQRISEHAREANMTV
                     VVSAMAGVTESLIRGAHAAGNGDREQWKNIARGLTRGHQEVANQLLSGSELTGVWPLL
                     AEQVQIFENLCSGFSLVGEVTPRALDTVSSLGEMMSANLLAAVLRSKGHRAEAIDAME
                     LIVTDDNFGNASPLFDETNARLCRRLKPLLQSGSIPVVPGFRGATRGGACTTLGRGAS
                     DYTATIVGSALDADEVWIWTDVDGVMTADPRLVPGARIIPEISYRETIELSFFGAKVL
                     HAKAVQPAMKKGITVLIRNTFSPSCLGTRIGPNAVGQPGVRAITAVSNASLFSVVGND
                     SLSFGHLAAKIFGALAPEEIPTLMATQSSAENVLCFAIHAAEEPRVRARLEKAFTLEM
                     RHDYVAAIDIMPKVGIVVAVGEKMKGTPGIAGRMFGALGRRGINVIAIAQGSSELSVS
                     LAVQSGEVAEAVRTIHGEFQL"
     gene            complement(9310..10524)
                     /gene="thrB"
                     /locus_tag="VN788_05365"
     CDS             complement(9310..10524)
                     /gene="thrB"
                     /locus_tag="VN788_05365"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXU19993.1"
                     /translation="MPVTVHIPTALQKFTGGEQSVELAAPLPSLLDQLAARFPGIAPH
                     LRDKHGALRHFLNIYVNDEDIRLLGAAAYQFRDGDDVLLVPSIAGGKPAAREAEVCVP
                     ATSANLGCALDCGAVAVNRYLRARARQRREPGVTLSYEGVNAERIPPDKSNLLIQAIQ
                     EIAASRGEELPGLHVQVESEIPVGAGLGSSAASIVAGLVLGAQLLGAKHEDDEILSLA
                     ARMEGHPDNVTAAYHGGFTIALLEKQSSRVLFAKALMPSDLRLIAIVPDIPLPTKSAR
                     AVLPPSYSREDVVHNLQRAVLLAAACFSGSFAFQPELFRDRLHQPYRSRLIPGLEVCL
                     EVRHPALLGVFLSGAGSSVLALVRNERAEAEVAERLAQGFQRHGLSTQALFLQAENRG
                     VRELAREGVWKA"
     gene            complement(10528..11799)
                     /gene="thrC"
                     /locus_tag="VN788_05370"
     CDS             complement(10528..11799)
                     /gene="thrC"
                     /locus_tag="VN788_05370"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXU19994.1"
                     /translation="MAELYQLRCRECGRLWGNAPRSYCEECFSPLEITYDYAALRDRV
                     TRDGIVARPSNLWRYAELLPLPEGFQSELATGFTPLVRAHRLGARLGVDSLYVKNDAV
                     CFPSLSFKDRVVAIALTAAKRFGFQVVSCSSTGNLANAVAAQAAREGLQACIFVPADL
                     EAAKILNTQVYGARLIRIAGNYDQVNRLCSQIADAYRWGFVNVNLRPYYAEGSKTVGF
                     EIAEQLGWRLPDNVVVPMAGGALIGKIAKAFRELTDLGLVDAKPVKFFGAQATGCSPI
                     SDAVKNSKEQFDPQKPNTIARSLAIGNPADGNYASAAIRASGGWAEDVSDPEIVSAIG
                     LLAETEGIFGETAGGVTLGVTQKLLAQGRIAPGGTTIICNTGNGLKTTESLAGRFELG
                     EPIPPRLADFERAMASAFAAATAGLPAVAGS"
     regulatory      complement(11947..12061)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(12119..12670)
                     /locus_tag="VN788_05375"
     CDS             complement(12119..12670)
                     /locus_tag="VN788_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HXU19995.1"
                     /translation="MCQAFAIPPTRKYQSEGGPGIRDIVELLTANSTSHAEDVATFLD
                     SVAYNWLIVGTDAHAKNYALLIGAEGRIRLAPLYDVASVLPYPDIDIEKVKLSMKLGG
                     EYRLRNITLHHWRKLAAELHVDPDAMTARVDSFAVRLSDHVSEIRRRMAEEGIAHPIV
                     ARLADALVARAAACRKLLHAALA"
     gene            12984..13427
                     /locus_tag="VN788_05380"
     CDS             12984..13427
                     /locus_tag="VN788_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXU19996.1"
                     /translation="MRKTRSMVVSMRLPTESGNRLKRLASRHGWTPSDASARLVEEGL
                     RRSEFAFIDFRDSAAGRQAYVQGSSLAVWEVMLLVQSYKASVEAAAKHLKWPEAKVQA
                     AVHYAKAFPEEIESALSENAATDFETLKRMLPQAARFGLVSVTKS"
BASE COUNT         2796 a         4275 c         3974 g         2784 t
ORIGIN      
        1 agaaaacaaa tctgttgcca actgggatgg taatcgcatc caataacaat catcgaaggc
       61 tccttcctac cgagtgcttc ggtcctccaa cccaaagcat actcggctac cgaacggagc
      121 cttcgttctt atccaatcaa ctcttggccc ggctggccat tcgcaccagc gatgatacgg
      181 tgctgcggcg tgtgaaggct ccggcgtcgc cagatccgag tgcccccgac ggaggttctc
      241 ggtgtggatg actgggcctg gcgcaaaggg cagtcctacg gaacgattct ggtcgatttg
      301 gagcagcgct ccgtgcggga ccttctggcg gaccgctcgg cggagagctt ccgggcatgg
      361 ctgcaacagc agccgcgtat tcgtgtgatt agtcgggacc gcggtggcat ttacgcggag
      421 ggcgcgcaat ggggtgctcc tgctgcccgg caagtcgccg accgctttca tctgttcttg
      481 ggaaattctg gatgcgcctt gaactagccc tttggtggct ggtgctgctg accggcccga
      541 gaagctatcc tttattggta cacttcataa aaaaaacgtc agcagttgcg cgcccgcgac
      601 atcgagatcc gataggcgat cacagcaagc aacgccaagg tcttgcggct gcctctccga
      661 gccaactcgc cgacttcgtc gcgctatcag tcggctgaag agatgtggtc agtggtccca
      721 ccaaaaggat cgtcggcgat gccatgcgag gagcaagagc gaggcctccg agctcggcgg
      781 cgcaaaaaga ctcatcaggt aacatcgcgc gggacacgac aagacaaggc gtcattgggt
      841 caacgccgct gcgcagcaac ctttcctgga ggtgggcgta atcttgcccc ggcatgtaga
      901 tagcgagcgt ggccccagaa gaaatgcccg atccaaagtc tggcagcgta tctggaccgc
      961 agctatgcgc cgtaagaaag attactctcg atgcggcgcg cctgtccgtc agggggactt
     1021 ttgccgaggc ggccgccgca aacgccgcag ttactccggg cacgacttcg aaatcaattc
     1081 ccgcgcgtcg aagcgcgtcc atctcttctc ccgcacggcc aaagaggagc gggtcgccgc
     1141 ccttgagtcg aactacggtc tgccctttcc gagcagtgtc aatcatacgc atgtgaatct
     1201 cttcttgcgt gatgtgtttt tgcccgcagc gcttgcccac attttgtttc agcgctccgg
     1261 gacgaatcat tcgcagaaca tccgccgaaa cgagatcgtc atgcaagacg acgtcggcgc
     1321 tctgcagcac gcgcaatgcc ttgcacgtca gcagttcggg atcgccgggt ccggcgccaa
     1381 ccagatagac gttcgcgctc atttttctcc aggcaacgga agttgccgcg ccatgcggag
     1441 caacatctcg cggcgccgcg ccggtgcgat ccgcaactgg aacaaccgct ctcgcgctgc
     1501 accgagtttc cgtacgctct ctgcgtattc cggcccgaat tcccgcgcaa agcgctcacg
     1561 caatcgctgc gccaacgccg gactcttgcc attcgtcgaa acggcaatct gcaaatcgcc
     1621 gcggcgaagc accgcgggat agaaaaaatc gcagcgcggc ggatcgtcca ccacgttgca
     1681 gaggacatcg cgcgagcgag cttcctggaa aacgaaatcg ttcaccgcca ccgaagatgt
     1741 cgcggcaacg accagaaatt tgccctcgag atcggcagga ctgaattttc gcggcagcca
     1801 cgcaattccg ccactcgcgg cccactcctt gatccgcgga gtcgcgcacg gcgcaaccac
     1861 ccacacggcg gcgcccgctt ggaccaagct ctcggtcttg gcttcaccaa ccgcacccgc
     1921 cccgacgacg aggcagggcc ggttttccaa cctgacaaaa agtgggaaga gggccatttg
     1981 cgctattcct ttgcgtggag accgcattcg gtcttttgga atccggccca acggccagcg
     2041 cgcggatctt cgccgggctg gacaggttgg gtgcagtgcg tgcaaccgat gctcggatag
     2101 ttgcggtcat gcaacgcgtt gtacggaacg ccgttcgcgc ggatgtagtc ccagatttgc
     2161 gcggaggtcc agtccgccag cggattgagc tttaccaacc cgaaagccgc atcccaatcg
     2221 agcttcttgg cgctggcacg cgcgggcgtc tgatctcgcc ggatgcccgt cacccaggca
     2281 tcaagttcag ccagtttggc cttcaatgga ttgaccttgc ggatctggca gcataggtta
     2341 ggctcgcggc tccagagtgc ctggccgtga agaaccgctt gctcctccgg cgtgagggcg
     2401 gggcgagtcc gttcgacatc aataccgtat ttgctctcga ttcgcgccat cagatcgcgc
     2461 gtctccgcaa aaaggaaatc ggtgtccagc gtgaatacgc gcaaaccggg ttgaattcgc
     2521 gcggcgatgt cgatgagcac gacaccttca gcgccaaaac tggatgcaac agccaggcgc
     2581 ggatggaagc ggtcaaaagc ccatcggagc agcttaccca ccgaccagac ctcggcttcc
     2641 gcttgctgcc ctagcacttc gtctttcatt gagatcatct tcgttcctct ccgtttggcc
     2701 gatggtcttt acctggcaac ccgcgggact aaggattcag cccacagaga cttttcacaa
     2761 gttcgagtgc ctgctggacc gcaatttcgt cgtgtgagtt ttgtcctgct ttgcgatctg
     2821 accacgccgg cctccccggc ataccagtcg cggaatgcga tcgtctccgg gaatttatag
     2881 ctggtatcca cgtgcaaaag cgggaaggga ggtttccggg ggaaaaacgc cttctgcgcc
     2941 aatcgcagca tcacggagga atccttgccg atcgagtaca gcatcaccgg cctcgcgaat
     3001 tccgccactg cttcgcgcaa gatgtgaatg ctctcggctt ccaaagcccg caaaaatggt
     3061 tcagatgtcc ggacgaaagg cgggtctcgc ctgttcccgt tgtactgggt acatctgcga
     3121 tttgtctcga ttcaatttcc gatccttgtc ccatttgttt tccttgaccg aaaaataaaa
     3181 aacccgcggc gttgatcgtc gcgggccatt ggagtttggt ttgggtgctt gcctaccctg
     3241 ccttgctcct cgatgcctgc ggcgcctccg tacaacaaca gcacgtgcta caacaggcgc
     3301 ggcacatacc gatacgagcg cccatcgata tccgtggctg gcgcttcgag cgcgttgtca
     3361 tttgccttcc ggcaatgcag atgttgcatg ccctcacgtc gttcaccctt gcacttcgtg
     3421 aggcgcatgc ccgggagcca cattgcgggg taccgcctcg acatttatcc cggccagaaa
     3481 actgcgcagc tcctggtcgg tgtaacgcgt aaagaattgc tgaaggtttt cttcgttgcg
     3541 ccgccgttcc agataagagc gcaatagccg ttcgatcgcc gccggaacct ccgtcgccgc
     3601 gcagcgatac ccgacggggc gcgcgatgga agcgagctgg cccaccgcgc cgcccacgca
     3661 gaaataatag gcatccacca tttctccgtt cacccggagc tttttgcctt cgatgccaat
     3721 gtccgcaatc cagtgctgtc cgcaactgtt ggggcatccc gtcacgtgca gcttcaggtg
     3781 ttgatcgaag ccgggaaagc gttcttcgag atcctccaca agccagagcg aaaacgcttt
     3841 cgtttccgta atggcgagct tgcaaagttc cgttccagtg cacgcgatca ctccgcggcg
     3901 gaagggtgac gcgtcgacgc gcagtccggc ggcggccagt tcatccgcca caaaggccgt
     3961 atatttcttc ggcacgccca cgagcagcaa gttttgcatg atcgtcgctc gcagttctcc
     4021 ggaggcaaac tgctccgcca ggtccgcggc gcgccgcatc tgcccgggca ggatcctccc
     4081 gcggagcacg ttcgcgccga cgtaaaacaa gtctggctgt ttctgtgcgt ggatgccgat
     4141 atggtcgcgg taaatgctcg tgggcgcttc ctccggctcg gccggatcga ggcggaagcc
     4201 aagacgccgc tccaactctt cctgaaattg ctccgccgtc caaccgtgat gcaggaacag
     4261 gaatttcagc cgggcgcgct cccggctttc gcgcagaaca tccgcgccgc gaaatacctc
     4321 tgcgacacct tgaaccacgg ggagaacctg atcctggcgg ataaatgcat tcagccgtac
     4381 cgccaggtgt ggtttggagg atagcccgcc gccgattcga agggaaaaac cggtctctgc
     4441 tccgccgtgg cgtttgcgcg tcaccgccgt cagtccaatg tcgttgatct cggggtatgt
     4501 gcaccagtcg cggcagccgg tgatcgagat tttgaacttg cgcggcaaat tataaaattc
     4561 cgaattcccg gccagcaggc ggctggcctg ttcgacaagc ggggaagcat cgcagatttc
     4621 ttgcgcgtcc actccggcca gcgggcagcc ggtgatgttg cggaccacgt cgccgcaggc
     4681 gcccagggaa gaaaggccgc agcgcgcgag actgtccagc acttcgggaa gcacctcaat
     4741 cgtgatccaa tgcagttgga tgttttgcct aacggtgatg tccgcgaagc ccaggccaaa
     4801 ttggtcgatc aggtccgcaa tcgtgcgtag ttgccgcgag gaaagaaatc cattggggat
     4861 gcggatacgc atcatgaaga agcgcacggc ccggccttcg ccgccgctgc cccccaccgc
     4921 tcccatgccg tcgccctgtg tataaatgcc ccaccagcga aaatacgtgc ccagccattc
     4981 cgccggaatc gattcatatc cctcgcgcgc gaagcagcgg atttccgcga ggccctccca
     5041 gggattcagt gatttcttca accgctcggc tttctgcgct ttggtctctc ttgcttccat
     5101 gatcgctcca aaaacaaaaa acccacctgc cagcttctcg tctggcggtg ggctcgaact
     5161 cagcttttca gcttggattc tgttctagct tgcctccggc ccagaccacg gacgcgcaca
     5221 acaacaaata caacacgcca tgcacatggt cacctggccg agattcatca tcacgccgtc
     5281 agattgcaga agcggggcct tgaagtcaac acttttcatt gaacggcgca atttcgaggc
     5341 tgaatggttt gaaaaaaggc cgcgttgcgc tttccacgcc gcttgttctg gccttcaagt
     5401 cttgcgcttt tccatgcgcc aactgctcga tccattggta ttggaaatgc ctgttactgg
     5461 cgagacgatt atctgtccgc tcacaaatgg gcaacagagt gtttggcgtg gcttggatca
     5521 cgcgggacaa tgtagccgtt ttcggcaagc aactcggcgt tcaagatggc ggcaccggcg
     5581 gccccgcgac cgaggttgtg tgagagcagc accaggcgaa tatccagtac gtggcacgca
     5641 cgaatgcgtc cgacgactac cgccatgccc tcgcccgcgt cgcggtcgaa tcgcgtctgc
     5701 ggacggtcag gttcttcgcg gacttcgatg ggacgcgcgg gcgccgaagg caatcccatt
     5761 cgttgcggct cgccccggaa ttcgctcagt gctgccgcta catccgcgag agaagctttc
     5821 cgtcggagct tgagcgagac tgcttccatg tgcccatcaa tcaccggtac gcgatgacac
     5881 tgcgcgctga tacggatgtc cgcaagctca atggcaccgt tgagcagcct gcccagtatc
     5941 ttccgcggct cggattccat tttttcttct tctccggaga taaacggaat cacgttatcc
     6001 atcgcatcga gcgatgggac ccccggaaag ccagcgccgg aaagcgcctg catcgtgacg
     6061 acggataacg cttcaagtcc gaaggcgtca tcaatcggct ttagcgccag caccagtccg
     6121 gctgccgagc aattgggatt tgtggcaata aaacccgaag tatatccgcg ctgccgacgt
     6181 tgcacggcaa cgagcgcaca atgctccgga ttcacctcgg gaactaggag cggcacattg
     6241 gcttccatgc ggtgattgcg gctgttgctg atcaccggat agcccgcgcg cgcaaagtcg
     6301 agttcggccg gccccgccac ggaagaatcg agcgccgaaa ggcagaagtc gcaatccaaa
     6361 tttgcctcga gcggttgcac cgtcagccca gcaacttcga ggggcaactg ctcgccaatt
     6421 cgccagcgcg ccacatcggc gtagtttttt ccggcggaac gttcggaagc agcaagcgcc
     6481 gtcacgcgaa accaaggatg ccgtcgcagg cgatggatga tttgctgtcc caccatgccg
     6541 gttgctccga gcacgcctac acgatactgt ttcatcgatg tgcgacctac cttgtttctt
     6601 aggggatcga gtgcgcttcc agcactcttc gcaaaccgct ctgcgttggt tcggcctgct
     6661 ctcgccagcc agacgcgttg ccctgcgacc gacgtttcag gatatagtcg gtgtctttca
     6721 actggttgcc ggtgaggatg gcgaccactt ccgctccttt ctcgatttgc cccgctcggc
     6781 aaagctttcg caaacccgct gcggttgccg ccgacgccgg ctcgcagcct accccgtccg
     6841 ccgcaaggac agccttggcc tccgagattt cctcgtccgt cacggattca cacaggccgt
     6901 ttgtccactg cagcacctgg cgagctttcc tccagttagc tggattgcca atacggatcg
     6961 cggtggcatc ggtgcgcgga tccgccacgg gaaccagttc cgttgagccc gaggccagca
     7021 ttttgtacaa cggattcgct ccggcggcct gcaccaccac tacgcgcggc actttctcga
     7081 tcaggctcag gaatctcaac tcacgaagac ccttgccaat cgccgctgtg ttgcccaggt
     7141 tgccaccggg gaccacgatg tattctggag gacgccagcc gcgctgttcc aacaactccg
     7201 caacgatcgt tttctggccc tcgatacgaa acgggttgat cgagttaacc aggtagaggc
     7261 ccatttctgg cgcgatcgcg ttaagcaact gaaacgcttt atcgaatgtc tcgcccacct
     7321 ccacaacgca cgcgccaaag tccatcgcct gcgccagttt ggcggaggag attccaccca
     7381 cggggacaac gagcattgct ttcatacccg cgcgggctgc gtaggcggcc attgaagacg
     7441 atgtgttgcc ggtcgacgcg caagccacca cacggacctt ctgcgaggcc gcctgcgtga
     7501 tgcacgcagt catacccagg tccttgaacg atccagtggg gttgttcccc tggtgcttga
     7561 tcgtcagtcc cacgccgcca gcccattccc ccgcgcgcag cgcttcaacc aacggagtcg
     7621 aaccctcgcc gagtgaaatg atggctaggc cgggatcgag aaatgggagc agctcgcgga
     7681 aacgccagac gccgctgcgg tccgcaggct cattgcttag cttgcgctgc gcccacttgc
     7741 ggtaaagtag ttccggttcg acgcgatcga aatcataggt cacatccaga agtccgccac
     7801 aagcgtcgca atggtgtccg ctgcgactgg actcctgccg gttccggcac gactcgtccg
     7861 cgcattggag aaagaatcga gacatgtgtc tgagtactta cagttgaaac tcgccgtgaa
     7921 tcgtccggac ggcctcggcc acttctcccg actgcaccgc cagagaaacg ctcagttcag
     7981 aggagccttg cgcgatggcg ataacgttga tgccacggcg tcccagggcg ccgaacatgc
     8041 gccccgcgat gcccggcgtt cccttcattt tttctcccac ggctaccacg atccccacct
     8101 tcggcattat gtcgatggct gccacatagt catgacgcat ctccagggta aacgcctttt
     8161 ccaatcgggc acgcacgcgc ggttcctccg cggcatgaat ggcaaaacat agcacatttt
     8221 cagcggacga ctgggtggcc atcaatgtgg gaatttcctc gggcgcaagc gcgccgaaga
     8281 tcttggccgc caagtggccg aagctcagcg aatcgttgcc aaccacggag aacaggctcg
     8341 cgttcgacac tgcggtaatc gctcggactc ccggctggcc gaccgcattg ggaccgatgc
     8401 gcgtccccag gcacgatggg ctaaaggtgt tgcggatgag caccgtgatt cccttcttca
     8461 ttgcgggttg tacggctttg gcatggagca ctttggcgcc gaagaatgaa agttcgattg
     8521 tctcgcggta ggaaatctcc gggatgatgc gcgcccccgg gaccaggcgg ggatcagcgg
     8581 tcatcacacc gtccacgtcg gtccaaatcc agacttcgtc tgcgtcaagc gcgctgccga
     8641 cgattgttgc cgtatagtcg gaagcccccc ggcctagcgt cgtgcaggcg cctccgcggg
     8701 tggccccgcg gaatccaggc accaccggga tgcttccgct ttgcagaaga ggcttcaagc
     8761 gtcggcacaa tcgggcattc gtttcgtcaa acaacggaga ggcattaccg aaattgtcgt
     8821 ccgtcacgat caattccatg gcgtcgatcg cttccgctcg atggcctttg gaccgcagaa
     8881 ctgccgccag cagattcgcc gacatcattt ctccgagact ggaaacggta tcgagcgcgc
     8941 gcggcgtcac ctcaccgacc agagaaaatc ctgaacagag gttttcgaag atctggactt
     9001 gctccgccag caaaggccac acgccggtca gttcgcttcc agacagcagc tggttggcta
     9061 cttcctgatg cccgcgcgtc aggccgcgcg cgatgttctt ccattgctcc cgatcgccgt
     9121 tgccggcagc gtgggcgccg cggatcagac tctccgtcac cccggccatc gcggagacga
     9181 ccacggtcat attggcttca cgggcatgct cgcttatgcg ttgcgccacg cgggaaatac
     9241 attccgcgtc gcccaccgac gttccgccaa acttcatcac cagaagttgg tgttctcctg
     9301 ttgctcggat catgctttcc acactccctc gcgggccagt tcccgaactc cacgattttc
     9361 ggcctgtaga aaaagtgctt gggtggacaa cccgtggcgc tggaatccct gtgcaagacg
     9421 ctcagccact tccgcctccg ctctttcgtt tcgtaccagg gctagaacgg aagagcccgc
     9481 gccgctcaag aaaactccca acagcgccgg atggcgcact tccagacaga cctccaaacc
     9541 tgggatcagc cggctccgat acggttgatg cagccgatca cggaatagct cgggttgaaa
     9601 tgcgaagctc cctgaaaaac acgcggcggc cagcaagacg gcgcgctgca ggttgtgaac
     9661 aacgtcctcg cgactgtaag aaggaggcaa caccgcacgg gcagacttcg tcggcagcgg
     9721 aatatccggc acgatggcga tcaatcgcag atcggaaggc atcagagcct tggcaaagag
     9781 cacgcggctg gattgttttt ccaagagagc aattgtgaag ccgccatggt atgccgctgt
     9841 gacgttgtcg gggtgacctt ccattctggc cgccagggaa agaatttcgt cgtcttcatg
     9901 tttcgcgccc agaagctgcg cacccagcac tagcccggcc acaatcgacg cggcactgga
     9961 accaagacca gctcccacgg gaatctcgct ttcgacttgc acatgaagcc ccggaagctc
    10021 ctctccccgt gacgccgcga tctcctgaat cgcctgaatc agcagattgg atttgtccgg
    10081 cggaatgcgc tcggcattca ctccttcata ggaaagggta acgcccggtt cgcggcgctg
    10141 gcgggcacga gcgcggaggt aacggttgac ggctaccgcg ccacagtcca aggcgcatcc
    10201 gaggttggcc gatgtggccg ggacgcacac ttccgcctct ctggctgcgg gcttcccccc
    10261 ggcaatcgaa ggcaccagca gcacgtcgtc accgtctcgg aactgataag cggcggcgcc
    10321 gagaaggcgg atgtcctcgt cattcacata gatattcaga aaatgtcgca acgcgccatg
    10381 cttatcgcgc aggtgcggcg cgattcccgg gaaacgcgcc gccagttggt caagcagact
    10441 cggcagcggt gccgccagct caacggattg ctctccaccg gtaaactttt gcagcgctgt
    10501 gggaatatgc acagtgaccg gcatcggtca gctccccgcc acggcgggca ggcccgccgt
    10561 ggcggcagcg aacgcgctcg ccatcgcgcg ctcgaaatca gcaagacgcg gaggaatcgg
    10621 ctcgcctagc tcgaatcgtc ccgccagcga ttcggtcgtt ttcaagccgt tccccgtgtt
    10681 gcagatgatt gtcgttcccc ctggggcgat gcggccttgc gcgagcagtt tctgggtgac
    10741 gcccagcgtg acaccgcccg ccgtctcgcc gaagatgcct tcggtttccg cgagcagccc
    10801 gatggccgag acgatttctg gatcgctcac gtcttccgcc catccgcccg aagcgcggat
    10861 agctgcgctc gcgtagtttc cgtccgctgg atttccgatc gccagcgacc gcgcgatcgt
    10921 gttcggcttt tgggggtcga attgttcttt gctgtttttc acggcgtctg aaattggcga
    10981 gcaccccgtc gcctgggcgc caaaaaactt cacaggcttt gcgtcaacca atcccagatc
    11041 cgtcagttcg cgaaatgctt ttgcgatttt cccgatcagt gccccgcccg ccatcggtac
    11101 gacgacgttg tccggcaatc gccagccaag ctgctccgca atctcgaagc caaccgtctt
    11161 cgaaccttcc gcgtagtagg gccgcaggtt gacgttaacg aaaccccacc gataggcgtc
    11221 cgcaatctgc gaacagagac ggttcacctg atcgtagttt ccggcaatgc gaatcagccg
    11281 cgcgccatag acttgcgtat tcaggatttt tgcggcttcc agatccgcgg ggacaaagat
    11341 gcacgcctgt aagccttcgc gcgcggcctg cgcggccacc gcgttcgcaa gattgcccgt
    11401 ggaggagcaa ctcacgacct ggaacccaaa ccgcttggca gcggtcagcg ctatggcaac
    11461 gacgcggtct ttaaacgaaa gcgacggaaa gcaaaccgcg tcgttcttaa catagagact
    11521 gtccactccc agccgcgcac ccagccgatg agcgcgcacc aatggcgtga agccagtggc
    11581 caactccgac tgaaaccctt ccggtagcgg cagcagttcg gcgtagcgcc acagattcga
    11641 gggccgcgca acgatgccgt cccgagtcac ccggtcgcgc agcgctgcat agtcataagt
    11701 gatttccagc ggagaaaaac attcttcgca gtacgagcgc ggcgcgttgc cccacaagcg
    11761 tccgcattcc cggcaccgca actggtaaag ctcggccatg gccatcctcg cacgacaccg
    11821 agtccgtgaa ttgcgggcgg cgtggaggac ggacttacac agcggaccgc cggtgcggca
    11881 gatgcaccag cgcgctgttc gtccttcaaa cttgggatga gaatatttag gagtgaaacg
    11941 cctaaattcc catgtccccc cgcgactcgg ggcgggaatt ggcaccggaa tgaagagctt
    12001 tgcgctccac cggttgccgt ggcgtcctag ggcccgttcc ctcggccact ctgcatgaaa
    12061 actcaggcct tgcagcttga gttttacgcg gcgaaagtac caaaagttca ctcgtgtgtc
    12121 aagcaagtgc agcgtgcagg agctttcggc aggcggcagc gcgcgcgacg agagcatctg
    12181 cgaggcgcgc gacgatggga tgggcaatcc cctcttctgc catgcggcgc ctgatttcgg
    12241 aaacatggtc ggacagtcgc actgcgaaac tgtccacgcg ggccgtcatt gcgtcaggat
    12301 caacgtgcag ctcggctgcc agcttgcgcc aatggtgaag cgtgatattg cgcaggcggt
    12361 attcgccgcc gagtttcatc gaaagtttga ccttctcgat gtcgatgtcc ggatagggga
    12421 gaacgctcgc cacgtcgtaa agaggcgcaa gacggatgcg gccttcggcg ccgatgagga
    12481 gagcgtaatt cttggcatgg gcgtccgttc cgacgatcag ccagttatat gcaaccgagt
    12541 ccagaaaagt ggcgacatct tccgcgtggc tggttgaatt tgccgtcagc aactcgacga
    12601 tgtcgcgaat gcccggccct ccttcgctct gatatttgcg agtgggaggg atggcaaatg
    12661 cctggcacat aggtaaatga caattttccc gtcttgtcgc gcgcaacgcg gcccatttcc
    12721 cggccttcaa taatcgcgat gagttctgtg gtcattttct ttttccgcgg gcggttgcaa
    12781 tgatggaatc gatatcgaca ggaggagtcc taccggtatt tttcgtattg gccggagatt
    12841 ccttgtccgt ggacaaccgg attcccagga ccccgatcgt gcgcagcaaa aggccgatct
    12901 cggctcaaaa agaacgcatg acgacaaatc acgcgagtgc ggaatagtgc tacaatagtt
    12961 acaggagtta caaaggagga cttgtgcgaa aaactcgttc gatggtcgtc agtatgcgtc
    13021 tgccgacaga gagcggaaat cgcctcaagc gtctggccag ccggcacgga tggacaccca
    13081 gcgatgcgag cgcgcgtctg gttgaagagg gcctgaggcg gtcggagttc gccttcattg
    13141 actttcgtga ttccgccgcc ggccggcaag cctatgtcca agggagcagc ctcgcagttt
    13201 gggaagtaat gctcttggtt caaagctaca aggcgagcgt ggaagcggcg gctaaacacc
    13261 tcaaatggcc ggaggcgaag gttcaagctg ccgtccatta cgcaaaggcc tttccggaag
    13321 agatcgaaag cgcgctctca gagaacgctg ctactgactt cgagacgcta aagcgaatgt
    13381 tgcctcaagc ggccagattt ggtctcgtat ctgtcacaaa gagttgaaat gctcaggctc
    13441 cttttggatg agcacatctc gccagctgtg gcaggcggtc ttcgccgcag gcatcgagca
    13501 ctctcggtgg tctgcatggt tgaatgggaa gatggagaat ttctagggca atcggactcg
    13561 gcatgcctgc gacaggccgc ggcgcaaggt ctgtcgcttg tgacctatga tcgaagaacg
    13621 ataccttcgt tgctcaaggt ttgggcggaa gaaggcagaa cgcacggcgg gttaatcttc
    13681 gtagacgaaa agactatccc accatccgat gtaggaaccc tcgtgcgagc cctaagcgcc
    13741 ctctggctta aaactgtgaa gtgggactgg agtgaccgaa tctgtttttt gcaaagctga
    13801 aagagttctg gcacacaacg acgctcagt
//
LOCUS       DATNDT010000163        10116 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_974548, whole genome shotgun sequence.
ACCESSION   DATNDT010000163 DATNDT010000000
VERSION     DATNDT010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10116
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_974548"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..108
                     /locus_tag="VN788_05385"
     CDS             <1..108
                     /locus_tag="VN788_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HXU19997.1"
                     /translation="GEKNPDAAWYYPAPKPAAAEIQNHVAFWKGVHVEA"
     gene            138..893
                     /gene="nth"
                     /locus_tag="VN788_05390"
     CDS             138..893
                     /gene="nth"
                     /locus_tag="VN788_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXU19998.1"
                     /translation="MAGEKKKSLARAPAKNQRTRKPPKSASGKTGAQKNGAGRGTNPA
                     RVRAILKGLDEAYPAATCALTHDNPFQLLISTILSAQCTDERVNQVTSTLYRKYPKPE
                     AFAYANPAELEQEIRPTGFFRNKTKSIMSASKLIVEKFDGKVPKTMEELLTLPGVARK
                     TANVVLGTAFGIASGVVVDTHVQRLAERLDLSKNTDPKKIEKDLMEVIPPERWILFSH
                     QLIWHGRRVCRARYPMCAECNLYKLCYFPHKTA"
     gene            complement(900..3188)
                     /locus_tag="VN788_05395"
     CDS             complement(900..3188)
                     /locus_tag="VN788_05395"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU19999.1"
                     /translation="MTKVGHNRTVKAILAALLAMGCLIPVHLRAQQSRPTTVPAPRES
                     QAPPRTNQLNPASVIRAQSNVVRIDIEVTGRDGKPIKGLRADQFVITDDGKGQKISSF
                     SYADIEAVEQAGAEDAKPLVVPVDSPAPVVSSNTSDTVADQIRNRRMIVMFFDLTSME
                     TDDLIRAHDAAVKFLKSQMKPPDLVAVVTFGSNLSVLANFTNNRATLEKAVAQLIPGV
                     SSGIANPLYAAAQNGESDVQQYTGDAYTADETEFNVFNTDQKLVAIEGLANVLASIPG
                     RKALVEFTGGITQTGEENRAELQAATDAANRANISIYSIDARGIYTTPPGGDVTTAAA
                     SGTSMFSGASVFHQTDQREDSRDTLATLSTDTGGRAFFDLGDLSDAFPRIQQENGGYY
                     LIGYDLPADAKRDGRWHAIRVKVKASGAHVRYRNGYYAPRDFQHLEKESRDQQLADAL
                     NSENPEVDLPIAVETSVFRLNDEQAYVPISTKLSPRALDWAQKHDRREAAFDFAVEVR
                     AVPSGQSVAALRDTINVHLDQQRFEQVNRSSLVYQGGVVLAPGNYRLKLVARENESGK
                     IGTFEEKLTVPPRPPARISLSSVLLSSQLVPVEKSTEAETKGQGFHAKLATTPLEISG
                     ERIVPSVTRFFTQRQTLYVFFQAYYPEKPEKNQAFDPSTLRAGLIFFRGGVQVNMTPL
                     IEPTQIDEKMHTASFRISLPLAKLPSGRYTVQAVVIAAGTQQSAFGRAYLALEQPPQA
                     PPSAAPSAAPASSRETQKSPNP"
     gene            complement(3203..6814)
                     /locus_tag="VN788_05400"
     CDS             complement(3203..6814)
                     /locus_tag="VN788_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU20000.1"
                     /translation="MCNSRVVKRVAAFTSMVVAMILAAPSGHHHGLLSGAERPNSQHE
                     TGRQRSQPESKPPASTTTFSVGGVAHTAEGAPVPGATVRFTNTDTNQIWVSWTDPSGK
                     FEFPALPAGPYRAEASQLGFVASSLEVRLGAGPAAPSMQFVLRVATLAQLATPAGDEK
                     VPHPATAARRRSNRNATAENGATSNAGSTAPAGRAGYRGRGQLPPGVLNAVTQGLAAG
                     GFQQTDLADESTGDNEEAGPPPTEMPSGGAGLSSDAFLLQGTVGMGLSPAGPGAAGGF
                     GAFGPGGMGPDFPGIGIPGGPAGGVGAQRGGGPGPGTVQGGGGAVFFARGAGGGGPRG
                     GGGGGRFFRQAVNRVRFSFYDRYENSAFDAKPYSLTGNQFPKISHYNERFGANIGGPL
                     AIPHIYNGKDRTYFFANYQHDTQQSPINTFSTVPTLDERQGIFCGTTLYQPFSSPGTP
                     FPSASPGCQQVTVSAISQALLAYIPAPNVPCTAGRCPVTRNYLLQATTPSNTDSVSAH
                     VLHTLNAKFNLNGGYNFNSVRENTLSNFLDTAGRESTRNQGFNLSLAHNWSPRFVENT
                     SINWSRSRAQILSDNSFINNIAGNLGINGIATTPIDYGLPLIQFTNFSSLNDPVPSLV
                     RNQTLRFDDSLTWVHAKHTMRFGGEVRRIQLNNNSNPIPRGQFTFTGLVTAQEGSNGF
                     PLEGTGNDFADFLLGFPYNTRVQFGNPSTHFRSWGFALYAQDDFRVSKTFTIQYGIRY
                     DAVTPPFELANQIANLDLNSSATAVALVTPGSPGTLNGVTVPGDIGPFHGTYPRALIH
                     GDYRNWAPRIGFAWQPGIKPKTVIRGGFSIFYDTSIYNTLAQRYLAYQPPFDVSHTVL
                     TTPQQTLTLSDGLLVQDQSSSTISNTGSIDPFYRDPYAQIWTLGTETSFSQNWILDLT
                     YTGTKGTDLETLRSPNRAPLGTPPNQTQLDLQIPNATSFYFLQSNANSIYNALQARLM
                     HRFTRGFMMQGIYTWGKSLDDAGSLGGTSATVQQQDGNIHAEYGLSSFDVRDQFRLLS
                     VYQFPFGERNRHTNHGWTRNIFGDWRLQNVFTWQTGTPYTVLVGGAATDNGTGANFSL
                     RADQVRNPNVGICGGSQAQFFNTSAFVAPPAFAYGNEHRGAVEGPCTISWNMSLAKTF
                     RFGPEQRHRVDARWEVQNLTNTVNYSGVGTSLGSRYFGEITSAGAMRTMDLMVRFNF"
     gene            complement(6876..7595)
                     /locus_tag="VN788_05405"
     CDS             complement(6876..7595)
                     /locus_tag="VN788_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20001.1"
                     /translation="MIGSFRKTYRNPGKDSSPYLPAILLGCALALMPLGILAQNAPAG
                     PIAPGASSDQAASARGPAPAASSGKHPNFAGTWQLNKKQSDDPHEKMREARDGQRGGG
                     GRWGGRGMGRGRRGQGQGNDMMKEYSKLTITQTDSRAKVTNESGRVLAFSSDGDQSAN
                     PPVSSDSNQSDSTERYSQPAQWQQDQLVVTAQTRGGSATRTYELSPDGKHLYLTTRIE
                     NPRLSQPVIIHFVYDPIKPSE"
     gene            7810..9159
                     /locus_tag="VN788_05410"
     CDS             7810..9159
                     /locus_tag="VN788_05410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20002.1"
                     /translation="MKIKIWTALHTGSRIPQLWGFRVTNLATDPLIDKQMLTEVERIL
                     RSQSLHNSETLKRLLKFLGEKSAVGEADQLKEYTIAVDALERPSTYDPRHDSAVRIQI
                     SRLRQKLAEYYRTEGKDNPLVIDLPKGHFKLTCGPRAPIIQPAASSAKTNAERTRTLV
                     AAFLLLTLTFAVAWGGYSIIKIRNAEAMSSAIDTRWTPDLEELWRPFLVTNRPMLVAI
                     EDPLFVELQRGSGIYYRDKSFDEWNGALGSPGVRALRKQFKDPNMQPSRYYTSFGEVN
                     AAFLIGRLLGAKESNLSVVKTSQLSWQQLADNNVVFVGVRAFFDGPLRSMPLQPELVS
                     VSKGIQNLKPRPGEPEMFSDRFSTAPTEEGEIYALVTHLPGPVGGSDVESFMSNRAAG
                     YVGAVQCFTDANFAKNLVANLKKAYGRIPRYYQVVLKVQFKDEVPTKTSFVLSRKLG"
     gene            complement(9184..>10116)
                     /locus_tag="VN788_05415"
     CDS             complement(9184..>10116)
                     /locus_tag="VN788_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU20003.1"
                     /translation="GLVHTYMAFMILRALFGIGMGGQWGIGASLAMEKVPAQRRGLLS
                     GLLQQGYAAGSLLAAICYLLLLGKWGWRPLFFVGCIPALFLVVFVYFRVRESEVWERS
                     GGQDWRGLAREFGSHWRLFVYLTVFMMMMLFASHGTQDMYPTFLQRQWHFTSTQSSAI
                     TAFSMVGAIIGGIVTGHLSDRWGRRRAMISAFVVGILIIPLWAYAPSKLLLILGAFLM
                     QFMVQGAWGVIPAHLTELAPDSVRASLPGLAYHCGLILSSLIVYIEAVFAEKASYATA
                     MALTALFVFALASIVAALGREQRGTVFGTSTDGG"
BASE COUNT         2226 a         2688 c         2995 g         2207 t
ORIGIN      
        1 ggcgagaaaa atccggatgc ggcatggtat taccctgctc cgaagcctgc cgcggcggaa
       61 atccagaacc acgtcgcatt ctggaaaggc gttcacgtcg aagcgtaatt ccggcgtgtg
      121 ctatagttcc tgactccatg gcaggagaga aaaagaagtc actggcgcgc gcgccggcga
      181 aaaatcagcg cacaagaaag cccccgaaga gcgcatctgg taaaacgggc gctcagaaaa
      241 atggcgcggg gcggggaacg aacccggccc gagtccgggc gattctgaag ggcctcgacg
      301 aagcctatcc cgcggccact tgcgcactta cgcacgacaa tcccttccaa ctgctaatct
      361 ctacgatcct ctcggcgcaa tgtacggacg agcgcgtcaa tcaggtcacc tcaacgcttt
      421 accggaagta tccgaagccg gaagcattcg catacgcgaa tccagcggaa cttgaacagg
      481 aaattcgccc cactggattt tttcgcaata agaccaaatc gatcatgagt gccagcaaat
      541 tgattgtcga aaaattcgat gggaaagtgc caaagacgat ggaggaattg ctgacactgc
      601 cgggtgtggc gcgaaaaact gcaaacgtgg ttctcggaac agcttttggg attgcgagcg
      661 gcgttgtggt ggatacgcat gttcaaagac tcgcggagcg gctcgaccta tcgaaaaata
      721 ccgatccgaa aaaaatcgaa aaagacctga tggaggtgat tccaccagaa aggtggattc
      781 ttttctctca ccagttgatt tggcacgggc ggcgcgtttg ccgcgcgcga tatccgatgt
      841 gcgctgaatg caatctctac aaactttgct atttcccgca caagacagcc tgaaaatctc
      901 taaggattcg gcgacttctg cgtttcacgg gaactggctg gcgccgcaga gggagccgcg
      961 gaagggggag cctgcggagg ctgctcgagc gcgagatagg cgcggccgaa tgcggactgc
     1021 tgggtccccg ccgcgatgac caccgcttgc acggtatagc gccccgacgg aagcttggcg
     1081 agcggcaggc tgatacgaaa cgaagcggtg tgcatttttt cgtcaatctg cgtaggctcg
     1141 atcagcggag tcatattcac ctgcactccg ccgcggaaaa atatcagtcc cgcgcgcaag
     1201 gtgcttggat cgaaggcctg atttttctcc ggtttctccg gatagtaggc ctggaagaaa
     1261 acgtagagcg tttgacgctg agtgaaaaat cgcgtgacgc tggggacgat tctctcgcct
     1321 gaaatttcga gtggcgtggt tgccaatttt gcgtgaaatc cctgcccctt cgtttcagct
     1381 tccgtggatt tttcaaccgg cacaagctgg ctggacagaa gcacggagct gaggctgatc
     1441 ctcgcaggcg gacgcggagg cactgttaac ttttcttcga aggtgccgat ttttccggac
     1501 tcgttttcgc gggcgaccag cttcaaccga taattgcccg gcgcgagaac taccccaccc
     1561 tggtagacca aactgctgcg gtttacctgt tcaaagcgct gttgatcgag atgcacattg
     1621 atcgtatctc ttaaggcggc tacggactgg ccggagggta cggcacgcac ctcgactgca
     1681 aagtcgaagg cggcttcgcg gcggtcgtgc ttttgcgccc aatccagcgc acggggggaa
     1741 agttttgtgg agatcggaac gtatgcttgc tcgtcgttga ggcggaagac tgaagtttcg
     1801 accgcaattg gcaaatcaac ctcgggattc tcggaattca aagcgtctgc gagctgttgg
     1861 tcgcggcttt ctttttcgag atgctgaaaa tctcgaggag cgtagtagcc attacggtag
     1921 cgaacatgtg cgcccgaggc tttcaccttc acgcgaatgg cgtgccagcg gccgtcgcgt
     1981 ttggcatcgg caggcagatc atagccgatg agatagtacc cgccattttc ctgttggatt
     2041 ctcgggaaag catcgctcag gtcgccgaga tcgaaaaacg ctcggccgcc ggtatcggtg
     2101 gaaagggtcg ctagcgtgtc acgcgagtcc tctcgctggt ctgtctggtg gaatacggag
     2161 gcgccgctaa acatggaggt gccgctggcc gccgcggttg tgacgtcacc tcccggcgga
     2221 gtggtgtaga tcccgcgagc atcgatggaa tagatggaaa tgttggcgcg attcgcggcg
     2281 tccgtggcag cttgcaattc agcgcgattt tcttcgccgg tttgcgtgat gcccccggtg
     2341 aattcaacga gcgcttttcg gcctggaatc gaggcgagga cattcgcgag accctcgatg
     2401 gcgactagtt tttgatcggt gttgaatacg ttgaattcgg tttcgtcggc tgtgtaggcg
     2461 tcgcccgtat attgctgcac atcggactcg ccgttttggg cggccgcata aagaggattt
     2521 gcaatcccgg atgagacgcc tggaatcagt tgcgcgacgg ctttttcgag agtggcgcgg
     2581 ttgttcgtga agtttgcgag cacggaaaga ttggagccga aggtgacgac ggcgaccaag
     2641 tccggcggct tcatttgcga cttgaggaac ttcactgcgg catcgtgggc gcgaatcaaa
     2701 tcgtccgttt ccatcgatgt gagatcgaag aacatcacaa tcatgcggcg gttgcggatt
     2761 tgatcggcga cggtgtcgga ggtgttcgag ctgacgacgg gcgctgggct atcgactgga
     2821 acaaccagcg gcttggcatc ttccgcgccg gcttgctcaa ctgcttcaat gtcggcatat
     2881 gagaagctgg aaattttctg gcctttgcca tcgtcggtga tgacaaattg atcggctcgc
     2941 aaacccttga tgggttttcc atcgcggcct gtcacttcga tatcgatgcg caccacgttg
     3001 gattgcgcgc ggatcacgga cgccggattc aattgattgg ttcgcggggg agcttgcgat
     3061 tcacggggag ccggaacggt agtcggcctg gattgctggg cgcgcaagtg cacgggaata
     3121 aggcagccca tagccaacag cgctgcgagg attgctttga ctgttcggtt gtgtccgact
     3181 tttgtcatgc tggcatcccg gactaaaaat tgaacctgac cataagatcc atggtgcgca
     3241 tggcgcctgc gcttgttatt tcgccgaagt agcgcgagcc cagggaggtg ccgacaccac
     3301 tgtaattgac ggtgttggta agattttgca cttcccagcg cgcatccacg cggtggcgct
     3361 gttccggtcc gaagcgaaaa gttttcgcga gagacatgtt ccaactgatc gtgcacggac
     3421 cttcgaccgc gccgcggtgc tcgttgccat aggcaaaggc gggcggggcg acaaaggcgc
     3481 tggtgttgaa gaactgcgcc tgagagccgc cgcagatgcc tacgtttgga ttgcgaactt
     3541 gatcggcgcg caaggaaaag ttggcgcccg tgccattgtc agtggcagcg ccaccgacga
     3601 gcacggtgta gggagttccc gtttgccagg tgaagacatt ctgaaggcgc cagtcaccga
     3661 agatatttct cgtccagccg tggttcgtat ggcgattgcg ttcgccaaaa gggaactgat
     3721 aaaccgacag gagccgaaat tgatcgcgga catcgaatga agacaatcca tattcggcgt
     3781 gaatgtttcc gtcttgctgc tgcaccgtcg ccgacgtgcc gccgagcgag ccggcgtcat
     3841 cgagcgattt tccccacgta tagatgcctt gcatcatgaa gccgcgcgtg aaccggtgca
     3901 tcaggcgcgc ctgcaaggcg ttgtaaatag agttagcatt tgactggaga aagtaaaagc
     3961 tggtggcatt cggaatttgg agatcgagtt gcgtttgatt cggcggcgta ccgagcggcg
     4021 cccggttcgg tgagcgcaaa gtttcaagat cggtgccctt cgttcctgtg taggtcagat
     4081 ccaagatcca attttgcgaa aaggatgttt ctgtgcccag cgtccaaatc tgcgcgtagg
     4141 gatctctgta gaagggatcg atactgcctg tgtttgagat ggtcgaggac gattggtcct
     4201 gtactaagag gccgtcggaa agcgtcaggg tttgctgcgg cgtggttagg acggtgtgcg
     4261 aaacgtcgaa aggcggctgg taggctaggt atcgctgagc gagcgtgttg taaatggacg
     4321 tgtcgtaaaa aattgagaag ccgccgcgga tgactgtctt aggcttgatt cctggctgcc
     4381 acgcgaaccc gatgcgaggc gcccaattgc ggtagtcacc gtggatcaac gcccggggat
     4441 aggtgccgtg gaacgggcca atgtcgccgg gaacggtgac gccatttagc gtgcctggcg
     4501 agccgggcgt tacgagagct acggctgtag cgcttgaatt caaatccaaa ttcgcgattt
     4561 gattggcaag ttcgaaggga ggcgtgactg cgtcgtagcg aatcccatat tggatagtga
     4621 aggtcttgct cacgcgaaaa tcgtcttgag catagagtgc gaagccccaa ctccggaagt
     4681 gggtgcttgg attcccaaat tggacgcgag tgttgtaggg aaaacccaag agaaaatccg
     4741 cgaagtcgtt tcccgtgcct tcgagcggaa agccattcga gccttcctgt gcggtcacaa
     4801 gtcccgtgaa cgtaaactga ccgcgcggaa tgggattcga attattgttg agctggatac
     4861 gcctaacttc tccgccgaat cgcatggtgt gttttgcgtg gacccaggtg agactgtcat
     4921 cgaaacgcag cgtctgattg cgcactagcg agggaacagg atcattcaag cttgaaaaat
     4981 tcgtaaactg aatcagtgga aggccgtagt cgatgggagt ggtcgctata ccgttgattc
     5041 ccagattgcc ggcgatattg ttgataaacg aattgtcgct caggatttgc gcccggcttc
     5101 ggctccagtt aattgaagta ttttcgacga atctgggaga ccagttgtga gcaaggctca
     5161 gattgaatcc ctgatttcga gtggactcgc gcccggctgt atccaggaaa ttggacagag
     5221 tgttttcgcg cacagaattg aaattgtagc cgccattcaa gttgaacttg gcgttgagcg
     5281 tgtgaagcac gtgagcactc acggaatcgg tgttcgatgg agtggtggct tgcagtaaat
     5341 agtttcgagt gacggggcat ctccctgccg tgcagggcac attcggcgct ggaatgtacg
     5401 ctagcaaggc ctgagaaatt gcgctgacag taacttgctg gcagcccgga ctcgccgagg
     5461 gaaatggagt gccagggctc gaaaaaggct ggtagagagt ggtgccgcaa aaaatgccct
     5521 gccgctcgtc aagcgtcggc acggtcgaaa atgtgtttat cggactttgc tgagtatcgt
     5581 gctggtagtt cgcgaaaaag tacgtgcggt ctttgccgtt gtaaatgtgc ggaattgcga
     5641 gcggaccgcc tatgtttgct ccaaaccgct cgttgtagtg gctgattttt ggaaactggt
     5701 tgcccgtgag tgaatatggt tttgcatcga atgctgaatt ttcgtagcgg tcgtaaaagc
     5761 taaagcgaac gcgattcacg gcttgacgaa agaacctgcc gccaccgcct cctccgcgag
     5821 ggccgcctcc cccggcccca cgagcgaaaa agacagctcc gccgcctcct tgcaccgtac
     5881 caggtcctgg ccctccgcct cgctgggcgc cgacaccacc ggcaggtccg cccggaatcc
     5941 cgatcccggg gaaatcagga cccattccgc cgggaccaaa tgctccgaaa ccgcccgcag
     6001 cgcccgggcc ggccggcgac agtcccatcc cgacggtgcc ttgcaagagg aacgcgtcgg
     6061 aagataatcc cgcgccaccg gaaggcattt ccgtgggagg agggccggct tcctcattgt
     6121 cgccagtcga ctcgtctgct aaatcggttt gctggaaacc gcctgcagct agcccctggg
     6181 tcaccgcatt tagtacgccc ggcggtagtt ggcctctgcc gcgatacccg gcgcgcccgg
     6241 caggcgcagt cgatcccgca ttcgatgtgg ccccattttc ggcagtggca tttctgttgc
     6301 tgcgccgtcg ggctgcggtc gctggatgtg ggaccttctc atcccctgcc ggggtggcaa
     6361 gctgcgcaag cgtggccacg cgtagcacaa actgcatcga gggcgcggct ggtccagccc
     6421 ccagacggac ctcaagagag gatgcgacga aaccaagctg gctcgcctcg gctcgataag
     6481 gtcctgcggg aagggcgggg aattcgaact ttcccgatgg gtccgtccaa ctgacccata
     6541 tttggttggt atcggtattc gtgaagcgca ccgttgctcc aggaaccggg gcgccttcgg
     6601 ccgtatgcgc cactccgccg acgctgaaag tggttgtaga tgctgggggt ttcgattcgg
     6661 gctgtgaccg ttggcgtcca gtttcgtgct gcgaatttgg ccgctcagcc ccggaaagca
     6721 gaccgtgatg gtgcccggac ggcgcggcga gaatcatcgc gaccaccata gacgtgaagg
     6781 ccgcaactct tttcaccacc ctactgttgc acaatgaccg ttcctccggc gcatgggcca
     6841 cagcaggcgg ctccttggag tatcgtcacc ggtagctact cgctaggctt aatcggatcg
     6901 tacacgaagt gaatgataac tggctgactc aatcgtggat tttcgatgcg tgttgtcaga
     6961 tagagatgtt tgccgtccgg agacagttca taagtccgag ttgcggagcc gccgcgagtc
     7021 tgcgccgtca ctacaagttg atcctgctgc cattgcgcgg gctgagaata tcgctccgtg
     7081 ctatcggact ggtttgaatc gctgcttaca ggcggattcg ctgattgatc cccgtctgaa
     7141 gagaaggcta gaacgcggcc cgattcattg gtcaccttag cccttgaatc cgtctgagtg
     7201 atggtgagct tgctgtactc cttcatcata tcgttccctt gaccttgtcc ccggcggcct
     7261 ctgcccattc ccctgccgcc ccatcggccg cctccgcccc tttgcccatc gcgggcttcg
     7321 cgcattttct catgcggatc gtcactctgt ttcttattga gctgccaagt tccggcgaaa
     7381 ttcggatgct tgccagaaga agcggctggt gctgggcctc gcgctgacgc agcctggtcc
     7441 gacgacgcac cgggagctat cggtccggct ggagcgttct gggccaaaat tccaagcggc
     7501 atcaaggcga gagcacatcc cagcagaatc gcgggcaagt aaggcgaaga gtccttacct
     7561 ggatttcgat aagttttgcg gaaagatcca atcatggagg aactccccag tgtcaggcaa
     7621 ttcggtgctg atgctcagcc ggccaacaga tgcgattccg tgctgagctc gatggagttc
     7681 cacaatttaa cgggggcggc cagttggcca agttagtccc tgcattgtct ggccattgtc
     7741 cgcggtccac cggatcggcg cgcttgtagg gactcgaacc cctaccatga ctgtgagtct
     7801 aagttaaata tgaagatcaa aatatggaca gcgttgcata ccggtagtag aattccgcag
     7861 ctttgggggt ttcgcgtgac caacctcgca acggacccac tgatagacaa gcagatgctg
     7921 actgaagtgg aacgcatcct gcgcagccag agtttgcaca attctgagac gctaaaacga
     7981 ctgctgaaat ttcttggaga aaagtcagcc gttggcgagg ccgatcaact aaaggagtac
     8041 acgattgctg tcgatgcctt ggagaggcca tccacttatg atcccaggca cgactcagcc
     8101 gttcgtatcc agatcagtcg gcttcgacaa aagctggctg agtactatcg gactgaaggc
     8161 aaggataatc ccctggttat tgatttgcct aaagggcatt tcaagttaac ttgcgggccc
     8221 cgggcgccga ttatccagcc tgccgcttcc agcgcgaaga cgaatgccga acggactcgc
     8281 accttggtag cagcgttcct actcctaacc ctaacgtttg ctgtagcgtg gggcggatat
     8341 tcaattatca aaatccgaaa cgcagaagcg atgtcctctg caattgacac gaggtggacg
     8401 cctgatttgg aagaactttg gagaccgttc ttggtcacga accggccaat gctcgtggcg
     8461 atagaagacc cactgtttgt agagttgcag cgaggatctg ggatctatta tcgagacaaa
     8521 tcgtttgacg aatggaatgg tgctcttggt tctccggggg tgagggccct tcggaaacag
     8581 ttcaaagatc cgaacatgca gccgagccgg tattatacga gctttggaga ggtgaatgcg
     8641 gcctttctta ttggaaggct gctcggcgcg aaagaatcca acctttcggt tgttaaaacg
     8701 agtcaattat cttggcagca acttgccgat aataatgtcg tattcgtggg agtgcgggct
     8761 ttttttgatg gacccttgcg ctccatgccg ctccaaccgg aactagtgtc cgtttctaaa
     8821 ggaattcaaa accttaaacc gcgacccgga gaaccggaga tgttctcaga tcggttctcg
     8881 acggcaccga cggaagaagg ggagatctat gctttggtca ctcacctccc cggcccggtc
     8941 gggggcagcg acgtcgagag ctttatgagc aatagagcgg ccggttacgt aggcgcggtt
     9001 cagtgtttta cggacgcaaa ttttgcaaag aacttagtcg cgaacctaaa gaaggcgtac
     9061 ggaaggatcc ctcgctatta tcaggtagtg ctcaaagttc aattcaagga tgaagttcct
     9121 acgaagacat ccttcgtgct aagccgcaag ttaggctagc gggttacgcg tcattctgtc
     9181 ctgctatccc ccgtccgtcg aggtgccgaa aactgttcct cgctgttctc tgcccagggc
     9241 ggctacgatt gaagcaagcg cgaaaacgaa tagtgcggtg agcgccatcg ccgtagcata
     9301 gctagccttt tcggcgaaca ccgcctcaat atagacaatt aagctcgaaa gtatcagccc
     9361 gcaatgatag gccaggccag gaagtgacgc gcggaccgaa tccggtgcaa gctcggtcaa
     9421 atgagctggg ataacccccc aagcgccctg gaccatgaac tgcatgagaa aagctcccaa
     9481 gatcaacagc aacttggacg gggcatatgc ccacaacgga attatcagaa ttccgaccac
     9541 aaatgctgag atcatcgctc gtcgcctgcc ccatcgatcg gagaggtgac ccgtcacaat
     9601 tcccccaata atcgctccaa ccatagagaa ggcggtgatc gccgaactct gcgtcgacgt
     9661 aaagtgccac tgccgctgga ggaatgttgg atacatatcc tgggtgccat gagaggcgaa
     9721 cagcatcatc atcatgaaga cggtcagata aacgaaaagt cgccagtgcg atccgaattc
     9781 gcgggctaga cctctccaat cttggccgcc cgaacgctcc cacacctccg attctctgac
     9841 gcggaaatag acgaatacaa ccaggaatag cgccggtatg cacccgacaa agaacagagg
     9901 acgccatccc cacttcccga gcagaagcag gtaacagatc gctgcgagca gactaccggc
     9961 tgcatagcct tgctgcaata gtcctgacag caaacccctt ctctgtgcgg gtaccttctc
    10021 catcgccagc gatgccccga tgccccactg tcctcccatg cctatgccaa atagtgctcg
    10081 aaggatcatg aaagccatgt aggtgtggac caagcc
//
LOCUS       DATNDT010000164         9378 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3173360, whole genome shotgun sequence.
ACCESSION   DATNDT010000164 DATNDT010000000
VERSION     DATNDT010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9378
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3173360"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            208..1101
                     /gene="pgeF"
                     /locus_tag="VN788_05420"
     CDS             208..1101
                     /gene="pgeF"
                     /locus_tag="VN788_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HXU20004.1"
                     /translation="MQVLEAPALTRTNWLVHAFSTRRVGGSQVRAPSSAKTRTSRQQA
                     KADGHALNLGFTAGDNRRRVLLNRKQFLQAIGADELSLVAIEQMHSDIVYRIDSAGGA
                     VGNHPQGDALLTNVPGILLAIQTADCVPILIADMKHRAVAAIHAGWRGTHLRIAAKAL
                     GRMRMEFGTSPQDTVAAIGPSIGPCCYEVGSEVAAAFQSQFPNAREWFDGPFDTLAAG
                     ENDPNWLPWLTMKAPGHQPPPPRVQLDLVAANRAILAEAGVPPAQIFQSGFCTSCRTD
                     LFFSYRRERITGRMMAVIGIR"
     gene            1257..1679
                     /locus_tag="VN788_05425"
     CDS             1257..1679
                     /locus_tag="VN788_05425"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU20005.1"
                     /translation="MADMKAPMPAEGAADRRRSHRVNIAMPILVRGIRGTQRFEEESQ
                     TVSVSAHGCNLRVTNPVARGQEIALVNKKTAEELPCAVTFIGQRDAGKIEIGVEFIEP
                     SPLFWRIAFPPEDWDPSERKRPGGARAPTTPITPPRRR"
     gene            1703..3217
                     /locus_tag="VN788_05430"
     CDS             1703..3217
                     /locus_tag="VN788_05430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20006.1"
                     /translation="MSTDSPYLPRKAIGGSPTWAETSALAIGFRFVGEYWLAVSGISV
                     LILAPCFWHREIVASDLGSHLYNAWLVQLIQRGELPGLWIAHQPTNILFDILLSNLGS
                     IFGLHAAEKLAVSFAVLIFFWGTFALISAAVRRPPWSLLPCIALVTYGWTFHLGFFNY
                     YLSLGLSFFSLALWWRGKGRDWIISLLLTPLVFMAHPLGLIWLVGAGVYIGLAKMLPH
                     RAYQWALCTAVAALLLAAHIFISHYYVADPGAKPFYAFNGADQLVLFGERYRLLARAL
                     LAFALVSIAADLIRRRHESALLRYYGIPIQLYLLAMFSVVVLPDAIHFPQPTATIALL
                     TERFTLITAVIGCCMLGAMQPRKWHFAAAALIASVFFVLLYQDTAIVNRMEAQVVRLV
                     SKLPPNQRVLATIEPFPGSRILNQHIIDRACIGHCFSYGNYEPGSRVFRVRATPGNPY
                     VLDSYELATETEEGEYLVQPRDLPLYEFYQCSEDGTNICMRPLNAGEYNGKPSE"
     gene            complement(3243..3902)
                     /gene="msrQ"
                     /locus_tag="VN788_05435"
     CDS             complement(3243..3902)
                     /gene="msrQ"
                     /locus_tag="VN788_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HXU20007.1"
                     /translation="MNKILTSKWTKVVVFVVCLGPAAWLVWRGFHQDLTADPIKYITH
                     FTGDWILRFLVITLAITPLRKILHVPQLIRFRRMFGLFAFFYAFLHFSTWIGLDKTFN
                     WTAMWQDVMKRPFITVGFAGFVLLIPLALTSTAWSIRRLGGKRWQMLHRLIYATAVLG
                     VIHYYWLVKSDVRKPLEYAFLVGILLAWRIGSWLMARRKRPVPRTAARQEPRAAETVR
                     Q"
     gene            complement(3912..4904)
                     /gene="msrP"
                     /locus_tag="VN788_05440"
     CDS             complement(3912..4904)
                     /gene="msrP"
                     /locus_tag="VN788_05440"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008330155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HXU20008.1"
                     /translation="MFIKKKPELTYADVTPKALYMGRRNFLLGVAATGMAVAGWKELP
                     GLLARGGTGGSVPVKLNGVKKWPYSTTEAETPIEKVTTYNNFYEFGVEKGDPSKNSKG
                     FVTSPWSVSVEGECAKPRKYTMDEIMKLAPLEERIYRHRCVEAWSIVVPWIGYSFNTI
                     VKLAEPTPKAKYVAFESYYDPKQMPLGRQAGIQLPYVEGLRLDEAMNSLVLLCVGMYG
                     ETLPNQDGAPVRMVIPWKYGFKSIKSIVKIRFVKSEPPTTWDLYRGFGGQPEYGFYAN
                     VNPHVDHPRWSQARERRIGPGFGLFNKMIPTQMFNGYGDQVASLYTGMDLRKNF"
     gene            complement(4986..7577)
                     /gene="glnD"
                     /locus_tag="VN788_05445"
     CDS             complement(4986..7577)
                     /gene="glnD"
                     /locus_tag="VN788_05445"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: protein motif:HMM:TIGR01693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0018177 - protein uridylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HXU20009.1"
                     /translation="MSTQSTPGHELRDLYSEGLARLQRDFASTGDGRAAVGGRADLVD
                     SLALRLWNTLIPEEETAKNLALVALGGFGRRRLFPYSDIDILFLQSGTEIESRVKGGI
                     RDLSQQMWDLRLKLSPATRTVSECEKFDPNNVEFAISLLDCRYLAGDRALFDRLHDQV
                     IPKLVARESRAIVQRLTDLTRERYNKFGDTVFHLEPNVKDGPGGLRDHNLAYWLALIA
                     AIEETNTWPDVKTLLPPSLRPKFDAALDFLASVRCFLHWRHGRDDNTLAWAAQDEAAA
                     QKIGVRTGNVPTASDWMRIYFNQARLIHRVAAQLLDEFPATRPSLYRQFQNLRSRLSN
                     AEFAVVDGRIFLQRQAAIEDTELPLRMFRFMARHGLTLSSATEHQVEEFVPAIAATPP
                     KGPELWHYLQEILLAPHAAEALRTMHSLGLLTALLPEFAVIDSLVIRDYSHRFTVDEH
                     SFVAIENLHALRKAQSKWDRGFAELLDELEQPDLLYLALLLHDIGKGTNPENHVAGSL
                     EVARTCLTRLELDAADRDTVFFLIERHLDLGAALRRDIFDPETIHQIAEKMATPERLK
                     MLCLLTYADIKAVNPEALTPWKTENIWQLYIGAANFLMRSVDERLHGHSDDELMHHLR
                     TLSPAAGKRLDDFLNGLPRRYLRTQAASDVIRHMEMARKLDHDPVQLELTRRRHWYDL
                     TLVTRDRPFLFARMCGALAAWGMNIVKAAAFSNDEGIVVDTFFFTDRFQTLELNLPEW
                     ERFKGSIHDVLLEKADLDRMLRDRLRSEKGTVPKIKVETRVEIDDACSKQSTLIEVIA
                     QDQLGLLHRISSQLVREDCNIEIALIETEGQTAIDVFYLTSKGAKLNPAHQEQLRAAL
                     IKELGAK"
     gene            complement(7813..8343)
                     /locus_tag="VN788_05450"
     CDS             complement(7813..8343)
                     /locus_tag="VN788_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase family protein"
                     /protein_id="HXU20010.1"
                     /translation="MGARNFLAIPAFLFVCFTVAAADSAPKSAHADILNAQGAKIGTA
                     RITSSGGGVKIAVKVDQLPPGSHGIHIHAVGKCDAPDFKTAGGHFNPTNAHHGVNNAE
                     NPHPHAGDLPNLTVGANGKGSASLVAKGVTLDDGANSLFHEGGTSLVIHAKADDLMSD
                     PSGNSGDRIACGVIEK"
     gene            complement(8448..8750)
                     /locus_tag="VN788_05455"
     CDS             complement(8448..8750)
                     /locus_tag="VN788_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014779988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HXU20011.1"
                     /translation="MADEKESGTQTVRMVKCVKLGRELPGLDRVPWRGEIGKRVYENI
                     SKEAWAMWVNHSKMLLNEFRLNPLDPNAQKIMEEQMEQFFFGEGSKLPEGYVAPRH"
BASE COUNT         1917 a         2811 c         2544 g         2106 t
ORIGIN      
        1 acagccttgt gttatcccag taggaacggc cgcgattata cctataattg agtgccctgc
       61 aggaatggca gaggttgtag aagacgagcc gccgcgcggc caaacgagat gatcggaaca
      121 gtgtcaaaaa agcgccgacc agaatctgga gctgctcgcc ctcggctttc agccaatgac
      181 aaatggaaac tacaagctgc agggggcttg caagtcctcg aagcgccggc gctcacgcgg
      241 acgaactggc ttgttcacgc tttcagcacg cgccgtgtcg gcggcagcca ggttcgggca
      301 cccagtagcg ccaaaactcg aacgtctcgc caacaagcga aggccgacgg ccacgctttg
      361 aatcttggct tcacagccgg agacaatcga aggcgtgttc tattgaatcg caagcaattt
      421 cttcaagcga tcggcgccga tgagttgagc ctcgtggcaa tcgagcagat gcattcggac
      481 atcgtctatc gaatcgattc agcgggtgga gctgtcggca accaccctca aggcgacgcg
      541 cttctcacca acgttccggg gatactcctg gccatccaga ccgcggattg cgtgccaatc
      601 ctgatcgccg acatgaagca ccgggccgtg gcggcaattc acgcgggttg gcgcggcacg
      661 catctccgga ttgccgcgaa ggccctgggt cgaatgcgga tggagttcgg tacttcgccg
      721 caagacacag tcgcagctat cgggccttcg atcggcccct gttgctacga agtcggctcc
      781 gaagtcgctg ccgccttcca atcacaattt cccaatgcgc gagaatggtt tgacggccca
      841 tttgacacgc tcgccgctgg tgaaaacgat ccgaattggc ttccgtggct caccatgaag
      901 gctccgggac accagcctcc gccgccacgc gtgcaacttg atctggtcgc ggcaaaccgc
      961 gcgattctgg cggaggccgg agttccaccc gcgcagattt tccagtcggg cttttgcacc
     1021 tcatgtcgca cggatctgtt ctttagctac aggcgggaac gcattacggg ccgcatgatg
     1081 gccgtcatcg gtattcgcta gcttcaatcg ccgccctcta tttcccaggc cggaaatctt
     1141 ctcaagactg cggtccacct attgaccagt tgtggcgatc ggaaacccgg cattccgtcc
     1201 aaccacagcc ggtggtgtag aatgctgttc gcatctcatc gaatgaggag atcctgatgg
     1261 cggacatgaa ggccccaatg ccggcagagg gcgcggcaga ccgccggcgc tcgcaccgcg
     1321 tgaatatcgc aatgcccatt ctcgtccgcg gcattcgagg cactcagcgg tttgaggaag
     1381 aatctcaaac tgtgtctgtc agcgcgcacg gctgtaactt gcgcgtgacg aatcccgtgg
     1441 ctcgcggcca ggaaattgcg ctggtaaaca agaagacggc cgaggaacta ccctgcgcgg
     1501 tcacatttat aggccagaga gacgccggca aaatagaaat cggcgtggaa ttcatcgagc
     1561 cgtcgccgct cttttggcgc attgctttcc caccggaaga ttgggatccc tctgagcgca
     1621 aacgcccggg aggtgcccgc gcgccgacca caccgatcac gccgccgcgc cgccgctaga
     1681 ccccgccaaa tgcggccctg atgtgagcac ggactctcct tacttgcccc ggaaagcgat
     1741 cggcggctcg cccacttggg ctgagacttc agcacttgcc atcggatttc gtttcgttgg
     1801 cgaatactgg ctagccgttt cagggatctc cgttcttatc ctggcgccct gcttctggca
     1861 ccgcgagatt gtcgcctcgg accttggaag ccacttgtac aacgcctggc tcgtgcaact
     1921 gatccagcgg ggcgaattgc cgggcctttg gatcgctcat caacccacca acatcctctt
     1981 cgacattctg ctgagcaacc ttggctcgat cttcggctta cacgcggccg aaaagctcgc
     2041 tgtatctttc gcggtgctga tctttttctg gggaactttc gcgttgatat cggccgcggt
     2101 tcggcgtcca ccgtggtctt tgctgccctg cattgcactg gttacgtacg gttggacgtt
     2161 ccatctcggc ttcttcaatt attatctatc cctaggtctt tcgtttttca gtcttgcgct
     2221 gtggtggagg gggaaaggtc gggactggat tatctcgctg ttgttaacgc ccctcgtttt
     2281 catggcgcat ccgctcggat tgatatggct ggtgggcgcg ggagtttata tcggccttgc
     2341 aaagatgctt ccgcaccgcg cttatcaatg ggcgctctgc actgcagttg cggctttgct
     2401 tttggcggca cacatcttta tttcgcatta ctacgtcgcc gacccgggag caaagccgtt
     2461 ctacgcgttc aacggcgcgg accagcttgt actctttggc gagcgctatc gcctgctcgc
     2521 gcgcgctctg ttagctttcg cgctcgtttc cattgccgcc gatctgattc gaagaaggca
     2581 cgaatccgct cttctacggt attacggcat ccccattcag ctttacctcc tagcgatgtt
     2641 ttcggttgtc gtgctgccgg atgcaattca ttttccccag cccacggcca ccatcgctct
     2701 cttgacggag agattcacat tgattaccgc cgtcattggc tgctgtatgt tgggcgcgat
     2761 gcagccgcgg aagtggcact tcgcggctgc ggcgttgatt gcgagcgtct ttttcgtgtt
     2821 gctttatcag gacacggcca tcgtcaaccg catggaggca caagtcgtcc ggttggtgag
     2881 taaacttcca ccgaaccagc gcgtgctagc cacgatcgaa ccgtttcccg gctcgcgaat
     2941 attaaatcag cacatcatcg accgcgcctg cattggtcac tgtttcagtt acggcaatta
     3001 cgagcctggc tcgcgcgtct ttcgcgtgcg cgcaaccccc ggcaatccct atgttctcga
     3061 cagctatgag ttggcgacag aaaccgagga gggagagtat ctggtgcaac cgcgcgatct
     3121 gccactctac gagttttatc aatgcagcga ggatggaact aacatctgca tgcggccgct
     3181 gaatgccggc gagtacaacg gcaaaccatc agagtaagcc ggatttcgaa gcgattgttc
     3241 gcttactgcc ggacagtttc tgcggcacga ggttcctgac gagcggctgt tcgtggcacg
     3301 ggacgctttc gtcgtgccat aagccacgag ccaattctcc acgctaatag tattcccaca
     3361 aggaatgcat attcgagcgg tttgcgaaca tccgatttca ccagccagta gtagtgaatt
     3421 acgccgagaa ctgctgttgc ataaatcagc cggtgcaaca tctgccagcg cttcccaccg
     3481 aggcgacgga tcgaccaggc cgtcgaggtg agcgccagcg ggataagcag cacgaaaccc
     3541 gcgaatccga cggtgatgaa cgggcgtttc atcacatcct gccacatggc cgtccaatta
     3601 aatgttttgt ccagcccgat ccacgttgaa aagtgcagga aggcgtaaaa aaaagcgaac
     3661 aatccgaaca ttcttcggaa gcgaatcagt tgcggcacgt gcaggatttt gcgaagcgga
     3721 gtgatcgcca gcgtgatcac caaaaatcga aggatccagt ctccggtgaa atgtgtaatg
     3781 tacttgatgg gatcagcggt cagatcctga tgaaatcctc gccaaaccag ccaggccgcg
     3841 ggcccgaggc acaccacgaa aaccaccact ttcgtccatt tgctggtcag aatcttattc
     3901 acgaagattc tctagaagtt tttgcgcaag tccatgccgg tatagaggct tgccacctgg
     3961 tcgccgtacc cgttgaacat ctgcgttgga atcatcttgt taaatagccc gaatcctggc
     4021 ccgatgcggc gctccctagc ctggctccaa cgaggatgat ccacatgcgg attcacgttg
     4081 gcataaaacc cgtattcagg ctgccccccg aatcctctgt acaggtccca ggtcgtcggc
     4141 ggctcacttt tcacaaagcg tatcttcaca atggacttga tgctcttgaa tccgtacttc
     4201 cagggaatca ccatgcgcac gggtgcgcca tcttgattcg ggagcgtctc gccatacatt
     4261 cccacgcaga gtagaaccag cgaattcatt gcttcgtcca atcgcagccc ttcgacgtac
     4321 ggcagctgga tgcccgcttg cctgcccagc ggcatttgct tgggatcgta atagctttcg
     4381 aaagccacgt acttcgcctt gggcgtcggt tcggccagct tcacgatcgt gttgaacgag
     4441 tacccgatcc agggcactac aatcgaccaa gcttctacgc aacgatggcg ataaattcgt
     4501 tcctccaacg gcgccagctt catgatttcg tccatggtgt acttgcgcgg cttggcgcat
     4561 tcaccttcca cggaaacgct ccatggagag gtcacgaaac ctttggaatt cttcgaagga
     4621 tcgcccttct cgacgccaaa ctcgtagaag ttgttgtagg tggtcacctt ctcgatcggc
     4681 gtctccgcct ctgtagtgct atacggccat ttcttaacac cgttaagctt caccggcacc
     4741 gatccacccg tgccaccgcg cgcgagcaac cccggcagtt ctttccatcc agccactgcc
     4801 ataccggtcg ccgccacacc gagcaaaaaa ttccggcggc ccatatataa cgccttgggc
     4861 gtcacatccg cgtaggttaa ctccggcttt ttcttgataa acatggggcc tcctttattg
     4921 ataatacgcg ccgtatatta gattcgatgc ccaaaatcgc cgaatggcat ggcaattcgg
     4981 caccttcact tcgcgcctaa ttccttgatc aacgcagcgc ggagctgctc ctgatgcgcc
     5041 gggttaagct tggcaccttt tgaggttaga taaaacacgt caatcgccgt ctggccctcc
     5101 gtttcgatca gcgcgatctc gatgttgcag tcctcccgca ccagttgcga gctgatccgg
     5161 tgcagtaatc cgagctggtc ctgcgcaatc acttctatca acgtgctttg cttggagcac
     5221 gcatcatcaa tttcgacgcg cgtctccacc ttgatcttcg gtaccgtacc tttctccgat
     5281 cgcaaccgat cgcggagcat tcggtccaga tctgcctttt ccagcagtac gtcgtgaatg
     5341 ctccccttga atctttccca ttccggcaaa ttcaattcca gcgtttggaa tcggtcggtg
     5401 aagaagaatg tatccactac gattccctcg tcgttcgaga atgcggcggc cttcacaatg
     5461 ttcattcccc acgccgccag cgcgccgcac atccgcgcaa aaagaaacgg ccggtcgcgc
     5521 gtgaccagtg tcagatcgta ccaatgccgc cttcgcgtca attccagttg gacgggatcg
     5581 tggtccagtt tcctggccat ttccatgtgt cggatcacat cgcttgccgc ctgcgttcgc
     5641 aggtatcgtc ggggcaagcc attcaaaaaa tcgtcgagcc ttttcccggc agcgggggaa
     5701 agcgtccgca gatggtgcat gagttcgtcg tcgctgtgtc cgtggagtcg ttcgtcaacg
     5761 cttcgcatca gaaaattggc ggcgccgata taaagttgcc agatgttttc cgttttccaa
     5821 ggtgtgagcg cttcagggtt taccgctttg atgtccgcat aggtaagcag gcaaagcatc
     5881 ttcagccgct cgggcgttgc catcttctcc gcgatctggt ggatcgtttc aggatcgaag
     5941 atatcccgcc gcaaggcggc gccgaggtcc aggtgccgtt cgatcagaaa gaagaccgta
     6001 tcccggtcgg cggcgtccaa ctccagccgc gttaggcacg ttctcgcaac ctccaagctg
     6061 ccggccacat gattctccgg gttcgttcct ttacctatgt cgtgaagcag cagagcaaga
     6121 tacagcaggt ccggctgctc gagttcgtcc aggagctccg caaaacctcg atcccatttc
     6181 gattgggcct tccgaagtgc gtgcaaattt tcgatggcca cgaagctgtg ttcgtccacg
     6241 gtgaaccggt gcgaatagtc tcggataacc agcgaatcta tgaccgcgaa ttccggaagc
     6301 aacgcggtga gcagacccag cgaatgcatg gtccgcagcg cctcggccgc atggggcgcg
     6361 agaagaatct cctgcaaata gtgccagagt tccggtccct tcggcggggt agccgctatg
     6421 gccggaacga attcctctac ttgatgttcg gtagctgagc tgagggtcag tccatggcgg
     6481 gccatgaagc ggaacattcg taagggcagc tcggtatctt cgatcgccgc ttgccgctgc
     6541 aggaaaattc ttccgtccac taccgcgaac tctgcgttcg aaaggcggga ccgcagattt
     6601 tgaaattggc ggtaaaggga cggccttgtc gccggaaact catccagaag ttgcgcggcc
     6661 actcggtgaa tcaatctggc ctggttgaag tagatacgca tccagtccga ggctgttgga
     6721 acatttccgg ttcgtacgcc gatcttctgg gccgctgctt catcctgcgc ggcccacgcc
     6781 agggtgttgt cgtcccgccc gtggcgccaa tgcagaaagc agcgcaccga cgcaaggaaa
     6841 tccaaagcgg cgtcgaactt tggccgcagc gacggcggca gcagcgtttt gacgtctggc
     6901 caggtgttcg tttcctctat ggcagcgatc agtgcgagcc agtaggccag attgtgatcc
     6961 cgcaaacccc cggggccatc ctttacgttc ggttccagat gaaataccgt gtcgccgaat
     7021 ttgttgtatc tctctcgggt aagatccgtc agtcgctgaa cgatcgcccg cgattcgcgc
     7081 gccacaagtt tcggaatcac ttgatcgtgg agacggtcga aaagcgcgcg gtctccggcc
     7141 agatagcggc aatcgaggag ggagatggcg aactcgacgt tattgggatc gaatttttcg
     7201 cactcggaca ccgttcgggt tgcgggactc aattttagcc gcagatccca catctgctgc
     7261 gataaatccc gaatgccgcc cttaactcgc gattctattt ccgtcccact ctgcagaaat
     7321 agaatgtcga tgtccgaata agggaaaaga cgccttcgcc cgaatccccc cagcgccacg
     7381 agcgccagat tcttggcagt ttcctcttca gggatcaacg tgttccacag gcgcagagcc
     7441 aacgaatcca caagatcggc tcggccaccc accgcagctc gcccgtcgcc cgtgctcgcg
     7501 aaatcccgct gcaaccgggc caatccctcg gagtatagat cgcgcagttc gtgccctggt
     7561 gtggattggg tggacatatc tttattgaat acaatttctt gctagcgtta agggtcgctc
     7621 actgcctgaa acgctgggcc tggaacccaa tgattctatg gttttttgca aggacggcga
     7681 gaaaaatctc tacccacgta accgccgatc cctgggacag cggaagtctg agtggaaagt
     7741 caccagcacg gatcacaccg gatgattctc cgacgcagtt tccggcggcg gatcttaacg
     7801 cgtcgacgcg gactattttt cgatgacccc gcaggcgatg cgatcgccag agttcccgga
     7861 agggtcgctc atcaagtcgt cggcctttgc gtgaatcacc agcgaagtgc cgccctcgtg
     7921 gaagagcgaa ttcgcgccgt catctagagt gacgcctttc gccacaagcg aggcgctgcc
     7981 cttgccgttc gctcccaccg tgagattcgg caagtcgccg gcgtgcggat gcgggttttc
     8041 ggcattgttg acgccatggt gcgcattcgt gggattgaag tggccgccgg ctgttttgaa
     8101 atcgggtgcg tcgcactttc cgaccgcatg gatatggatc ccgtggcttc ccggcggtaa
     8161 ctgatccacc ttcaccgcaa tcttcactcc gccgccgcta gaggttatgc gcgccgttcc
     8221 aatcttcgca ccttgggcgt tcagaatatc cgcatgcgcg gacttcgggg cggaatccgc
     8281 ggccgcaacc gtgaagcaga cgaaaagaaa cgcgggaatt gccaggaagt tgcgagcgcc
     8341 catgatgttc cctccagttg ggctggcggg ctaggagcca gcgactggca ttctatcacc
     8401 gggcaaggtc gctaggtagt ttggagcctc cacgatcagc ggaggcatca gtgccttggg
     8461 gcgacgtaac cttcgggcag cttcgaacct tctccgaaga agaattgctc catctgctct
     8521 tccatgatct tctgggcgtt ggggtccagc gggttcagcc gaaactcatt gagcagcatc
     8581 tttgagtggt ttacccacat cgcccacgct tctttcgaga tattctcgta gacgcgcttc
     8641 ccgatctcgc ccctccacgg cacgcggtcc aacccgggca attctcgccc caactttaca
     8701 cacttcacca tgcgaacggt ctgcgtgcca gattccttct catcagccat ctcgaatgcc
     8761 tcccaagaac tcagttccat cttatcttaa aaaagccacg acgccgccag caactcccga
     8821 tcggatagtt ggctactttg gattatggat ttacggacga agcgtggtgt gctttgcccg
     8881 gtggaagggc aggcttcagc cctgccacaa aacgatagcg cgaagcgcct tcatagatgc
     8941 cctgagcgct ggcgaagggt gcgctgcctt tctcgcaaca tgtactcgca ttcccgctca
     9001 tttccgatgg catgcaagtt tcttgtagac taggttgcaa agactcaagt gcacctctgg
     9061 aaatttaaag tagcccactg ccgccgaatc gtcggcgtgc tccgcttgta cgaaccgtcg
     9121 ctccgcatgt agcgattgct tgaacgcccg acaataggta aactcgcgcc ctcgatttgc
     9181 gcagtcatcc aggaaatgag gtccgctatg caaatgaaac gggtccgtct cgccgccggg
     9241 catttggccg ccgtgatcgc attggctgcg gcaatttcat cgccgctccg cgcgcagcag
     9301 gccaaagccg aagactaccc gccgcccaag cccgcctttc ccggccagac gaacgctccg
     9361 cctcccgcaa agaaatca
//
LOCUS       DATNDT010000165         7926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1481834, whole genome shotgun sequence.
ACCESSION   DATNDT010000165 DATNDT010000000
VERSION     DATNDT010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7926
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481834"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            418..1353
                     /locus_tag="VN788_05460"
     CDS             418..1353
                     /locus_tag="VN788_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXU20012.1"
                     /translation="MAWAGTGTLCVMQGGILKSFALSAMNKHDVSSMHADFAFAQISD
                     SHIGFNKAANPDVTATLREAIAKLNALPQAPEFVLHTGDLSQLSKADEFDTLDQSLKS
                     VRTGKIFYVPGEHDVLNDNGQQYRERYGKDSKGDGWYSFDHKGVHFIGLVNVMNLKAG
                     GLGDLGHDQLKWLEDDVKHLKHNTPIVVFAHIPLWTVYPQWGWGTDDSEQALSYLKKF
                     GSVSVLNGHIHQVMQKVEGNVTFHTAMSTAFPQPAPGQAPSPGPMKVPEEKLRSVLGV
                     TNINFVRGKHTLAIVDSTLQDNSTEGMPAQGEMRQ"
     gene            1416..1712
                     /locus_tag="VN788_05465"
     CDS             1416..1712
                     /locus_tag="VN788_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin family copper-binding protein"
                     /protein_id="HXU20013.1"
                     /translation="MNRPNYCVRAAEGQPAASTIVAVDNFTFTPKEITVPKGTTVTWV
                     NHDDVPHTVVSTDKKFKSRALDTDEKFSFTFSDSGIYGYFCSVHPVMRGKVIVR"
     gene            1824..2282
                     /locus_tag="VN788_05470"
     CDS             1824..2282
                     /locus_tag="VN788_05470"
                     /inference="COORDINATES: protein motif:HMM:NF016153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HXU20014.1"
                     /translation="MRGFLGTPASFSADLNLVVQIAMGIALLAGALLARSKRYSAHGA
                     CMAAVLLLNLVMIGLVMWPSFHELVLPRIPARLARRYYAVATIHGALGAATELFGLYI
                     LLVAGTQVLPKTWRFQRWRLWMRVELAIWWVVVLSGIGTYFLWYGTPHMP"
     gene            2589..3146
                     /locus_tag="VN788_05475"
     CDS             2589..3146
                     /locus_tag="VN788_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU20015.1"
                     /translation="MQDETGGASGQRSFDEVVLPHLDAAYNLARWLVRNPHDAEDVVQ
                     EACLRAVKFFEGYHGGDARAWLLRIVRNASYSFLEKRRPADSAQEFDERIRVPSVERP
                     DAEMLLVRSAESQKLQGALEQLPIAFREALILRELEGLSYKEIAEVMEIPIGTVMSSL
                     ARGRAQLRKSLSHVRERELQRGVRG"
     gene            3130..3936
                     /locus_tag="VN788_05480"
     CDS             3130..3936
                     /locus_tag="VN788_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXU20016.1"
                     /translation="MACADKVLLLHAYVDGELDLVRSLEIEEHLKACPACTEELAGHR
                     SLRSSLQSGDLYQRAPQALKDRIRPTAVGEGAPAAAPVANKAGWVASTRPIRRRPIFE
                     WLAIAAAILIAFAVGSRMVPGIASPRPDLVAEEAVASHIRSLQPGHLMDIQSTDQHTV
                     KPWFDGKLDFSPPVRDFSDQGFPLVGGRLDYLGNRNVAALVYQRRKHLINVFVWPENF
                     ADVANSSSSEAQRFVNGYNFLGWRYGTLHICAVSDVSVADLRQLKELLDQ"
     gene            complement(4165..4872)
                     /locus_tag="VN788_05485"
     CDS             complement(4165..4872)
                     /locus_tag="VN788_05485"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012992129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXU20017.1"
                     /translation="MIPLKDMTPRRSIPFVTLLLIAINAIVFMHQISLPPTATDAFIK
                     TYGLIPAKISLALAGRHYTMEQALLPLFTCMFLHGGFLHILGNMWFLWIFGGNVEDRF
                     GSLPYLVFYLVCGIGSGISQVLFSWGSHIPSIGASGAISGVLGAYIVFFPKSRILTLV
                     PLFIIFFLWRIPAIIFIGLWFAMQFLSGVVSLGQANVGGVAWWAHVGGFLVGMLIAGI
                     TGGSKRAPTSSWTSSSA"
     gene            complement(4869..5873)
                     /locus_tag="VN788_05490"
     CDS             complement(4869..5873)
                     /locus_tag="VN788_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HXU20018.1"
                     /translation="MNPARAFGSVDVVGVGINATDTIIRLPHFPAHDSKVEIRSSEVK
                     AGGQVASAMVACQRWGLSTRYVGKVGDDAAGKMQIGEMDREGVDARWIIAGGCASQSS
                     YILVDEASGERTVLWNRDSSIALRTEDLRREWISGASALLVDGHDTEAGAQAARWARE
                     EEIPVVGDFDNCYPGVEALLEYTDFAIASKNFAERLTAEKDLLKALPQICRRFKCRMV
                     GATLGKMGALIWDGEQFLLCPGFRIRAADTTGAGDVFHGAFLFGLVRGWQPEEILEFS
                     CAAAALNCTATGARGHIATLEEIENFRRGASRSEIAYAPGSLRAAAIAATKPSCESKP
                     "
     gene            6068..6820
                     /gene="recO"
                     /locus_tag="VN788_05495"
     CDS             6068..6820
                     /gene="recO"
                     /locus_tag="VN788_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HXU20019.1"
                     /translation="MPLHESEAIILQRYALGESDRLVSFFSRTMGKMRGVAGGARKPK
                     SRFGATLERLSYIRIWFFERETRELVRISQCEMIESFLDAFSDYPSSVALALFSEITE
                     TVLPDREPSDANFRLLLLAAQAVKRTGKPDLPLAYFALWTVKLGGWLPSLTRCAQCGA
                     ALDADAAAYFVPSVSAAVCGKCRKPGMRMLSPAAQGAARKVLEERLDRLTEKSDAPSP
                     RAARELADLMLDIIERQIDRKLKARELLESHA"
     gene            6867..7811
                     /locus_tag="VN788_05500"
     CDS             6867..7811
                     /locus_tag="VN788_05500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HXU20020.1"
                     /translation="MTKTDSKRRGLAFQDLLLKLEHYWTSRDCILQQPYDIEVGAGTM
                     HPETFLRVLGPQPYRVVYAQPSRRPTDGRYGENPNRLYKHTQLQVILKPSPSDIQDLY
                     LESLSAVGINLKEHDVRFEEDNWESPTLGAWGIGWQVLLDGLEITQFTYFQQCGGIDL
                     DPISVELTYGLDRIAAYLQGVDNVFDVRWSNTMTYGQVRLAEEQQFSAYSFDYADAAS
                     TRKQFDLNEAEAGRLLSKWGAASKKDAIETKRFPILQAYDLCLKCSHLFNVLDARGAI
                     SVTERAGMIGRVRKLATQVARAWLDQRAAVEATAGVVA"
BASE COUNT         1778 a         2258 c         2178 g         1712 t
ORIGIN      
        1 cacccccgct gtatccgata gccttgccct gcgctggaac gggtaccccg ttgtacgaac
       61 ttttcatcga ttgccatctc ctatgctgtc tagcgatgcg aggacggaac caaatacctt
      121 agcacaatcc gcgcatgctg tacccatcgc aaccgcggcc cggtgcgcaa tttcccaagg
      181 aatttgacgg cgctttaaat catacggacg cctcagggcc gggcactggt ttcaattttc
      241 tgctcaaact tgctattcgc gggaatattc ccccgcatag cccagtctat acgagggcag
      301 ggcaagcgcg gcccgaagtg agtgggcggc taaacgaaag tgaggcgttc ggtgggcaac
      361 atcatcaagg aaaacttgaa ccccgacggc gttgaccgcc ggggcttctt gaagtgcatg
      421 gcgtgggcgg gaacgggaac actctgcgtg atgcagggtg gcattctgaa atccttcgcg
      481 ttaagcgcga tgaataagca cgacgtgtct tcaatgcatg cggactttgc cttcgctcaa
      541 atcagcgatt cgcacatcgg cttcaacaaa gccgcaaatc cggacgtgac ggccaccctg
      601 cgcgaggcca ttgcgaaact caacgccctt ccccaagcgc ctgaattcgt gctgcatacc
      661 ggagatctca gccagctctc aaaggctgac gaattcgaca cgctcgatca atcccttaaa
      721 tcggtgcgta ccggcaagat tttctacgtt cccggtgaac acgatgtgct gaacgataac
      781 ggccagcagt atcgcgagag gtacggcaag gacagcaagg gagacggctg gtatagcttc
      841 gatcacaaag gcgttcactt catcggtctc gtcaacgtga tgaatctaaa agcgggcggc
      901 ctcggcgatc tcggccatga tcagttgaag tggctcgaag atgacgtcaa acacctgaag
      961 cacaacacgc cgatcgtggt tttcgctcac ataccgctct ggacagtcta tccccagtgg
     1021 ggttggggca cggacgatag tgaacaggcc ctttcatatc tcaaaaaatt cggatccgtt
     1081 tcggttctca acggacacat ccatcaggtg atgcaaaagg tcgaaggcaa cgtgacgttc
     1141 catacggcca tgtccaccgc ctttccgcag cccgctcccg gccaagcgcc ttctccaggg
     1201 ccgatgaagg ttcccgaaga gaagttgcgg agcgtgcttg gcgtcaccaa catcaacttc
     1261 gtacgcggta agcacacgct cgccatcgtc gattcaacct tgcaggacaa ctcaacggaa
     1321 ggaatgccag ctcaaggaga gatgcgtcaa tgaaaaacta cgcgttaaga attgccctgt
     1381 gtgccagttt gattggggcg gccgctgttg tcgcgatgaa tcgtccaaat tattgtgttc
     1441 gcgcggccga aggacagcca gccgcttcga cgattgtcgc agtcgataac ttcactttca
     1501 cacctaagga aatcacggtg ccgaaaggga cgacggtaac gtgggtcaac cacgatgacg
     1561 tgccgcacac cgttgtcagc acggacaaga aattcaagtc cagggccttg gatacggacg
     1621 agaagttttc cttcacattc agcgacagtg gcatttatgg atatttttgc tcggtgcacc
     1681 cggtgatgag aggtaaggtg atcgtcaggt aacgaaaaaa cacatctgcc atttcgcttt
     1741 atcgagatct agtgatgcgg ctcaaatgct gcgaccgagc gcgccccgct aagtcgaccg
     1801 ttgcgtctcc acggagtcct tcaatgagag gttttctggg cacgccagct agtttcagcg
     1861 cagacctgaa cctcgtcgtt cagatcgcga tggggatcgc gctacttgct ggcgcgcttt
     1921 tagctcgttc aaagcgctac tcggcccacg gcgcgtgcat ggcggccgtt cttcttctga
     1981 accttgtgat gattggcctc gtgatgtggc catcgttcca tgagctggta ttaccgagga
     2041 ttcccgcgcg tcttgctcgg cgatactacg ctgtcgccac cattcatgga gccctggggg
     2101 ccgcgacgga gttgtttggg ctttatattc tcctggtagc tggcacccag gtcctcccga
     2161 aaacgtggcg ctttcaacga tggaggcttt ggatgcgcgt tgaacttgcg atttggtggg
     2221 tcgtcgtgct ctcggggatt ggcacgtatt tcttgtggta tggcacgcca cacatgccct
     2281 gatcggtcct cactcagaac tagccaggcg agctcatttt tgttcttgca atacctaaac
     2341 gaaagcgtga ggccctgaag ccgtgtcgct ctaagcccga acataaaaat tcagggctta
     2401 tcaaaaggtt agggaagccc tgcgaagcga tttgggggcg aaaaaccgcc tcgcagagta
     2461 agggagaaac tgctgcgtgt ttgagtgtga gactcccggc agcgtcaata tattcccgtc
     2521 gcgcccgaga tatcgcggga ataaaatcgc ttggcatttg gtctcaacac ctgagagtct
     2581 acggctctat gcaagacgaa acgggcgggg catccggtca acggagcttc gacgaagtcg
     2641 tgctgccgca tctcgacgcg gcgtacaacc ttgcgcgctg gctcgtccgc aatccgcatg
     2701 acgccgaaga tgtcgtccag gaggcctgcc tgcgcgcagt aaaattcttc gagggctatc
     2761 acggaggcga cgctcgcgcg tggcttcttc gcatcgtgcg taacgcctcg tactcgtttc
     2821 tcgaaaagcg ccgcccggcc gattccgcgc aggaattcga tgaaaggatt cgtgtgccct
     2881 cggtggaaag gcccgatgcg gagatgcttc tggtgcgttc ggccgaaagc cagaagcttc
     2941 aaggagcact tgaacaatta ccaatagcct ttcgcgaggc cctgattctc cgcgagcttg
     3001 agggtctctc ctataaggag attgctgagg taatggagat tcctattggg acggtaatgt
     3061 caagcctggc ccgcgggcga gcgcaattgc ggaagagcct ctcgcatgtg cgcgaaagag
     3121 agttgcagcg tggcgtgcgc ggataaagtg ctgttgctcc acgcttacgt agacggcgag
     3181 ttggatctcg ttcgcagtct cgaaatcgag gaacatctga aggcgtgccc cgcctgcacg
     3241 gaggaactcg ccggtcatcg aagtctgcgt agctcgttgc agtccggcga cctctatcag
     3301 cgggctcccc aggcgctaaa agatcgaatt cgccctactg cagtgggtga gggcgcgcca
     3361 gccgctgcgc cagttgcaaa taaggcaggt tgggtggctt caacccgacc catccggcgg
     3421 cgtccgatct tcgagtggtt ggcgatcgca gccgccattc ttattgcctt cgcggtaggc
     3481 tcgagaatgg ttcccggtat agcgagtccg cgaccggatc tcgtcgccga agaagctgtc
     3541 gcgagccaca ttcgctcatt gcagccggga catctcatgg atatccaatc caccgatcag
     3601 cacaccgtga aaccgtggtt tgacgggaaa ctcgattttt cgccgcccgt gcgtgacttc
     3661 agcgatcagg gatttccact cgtcggtggc cggctcgact atctcggtaa tcgcaacgtg
     3721 gcggcgctgg tctatcagag gcggaagcat ctcatcaacg ttttcgtgtg gcccgaaaat
     3781 ttcgctgacg ttgccaactc ttcttcctcg gaagcgcagc ggttcgtgaa cggctacaac
     3841 tttctcggct ggcgatacgg caccttgcac atctgcgctg tttctgacgt gagtgtagcg
     3901 gatctgcgcc agcttaaaga actccttgac caatgatttc gcacgcgatc aggcacatga
     3961 agaacgcgtt catgcgattt tgaaaactta aagtcgaatc tacccgtgcg cgctttccag
     4021 ccgcgctgct caacctcaac tggaactgtc ggcaagccga ctcgccgacg ttatgaattg
     4081 gtttatgtag ctcaccccgc cttcacgagg tgaggttttt cggcttcaat cggcttgcac
     4141 atcaagccat gactctgcca gtttctacgc gctgctactt gtccagcttg atgtcggcgc
     4201 gcgcttggag ccgccggtaa tccccgcgat cagcatgccg accaaaaatc ctcccacgtg
     4261 cgcccaccac gccactccgc ccacattcgc ttggcccagc gaaaccacgc cgctaagaaa
     4321 ctgcatcgcg aaccacaggc cgataaaaat aattgccgga attcgccata ggaaaaaaat
     4381 gataaaaagc ggcaccagcg taagtattcg cgacttcggg aagaatacga tgtacgcccc
     4441 cagcactccc gaaattgctc cactcgctcc aatcgacgga atgtgcgagc cccaggaaaa
     4501 tagaacttgc gagatccccg atccaatgcc gcacactagg tagaaaacca ggtagggaag
     4561 tgagccgaat cggtcctcca cattgccgcc gaagatccac agaaaccaca tgttgcccag
     4621 aatatgaagg aagccgccat gcaagaacat gcaagtgaag agcggcaaca gcgcctgttc
     4681 catcgtgtag tggcgtccgg ccagcgccag cgaaattttc gctggaatca gtccgtacgt
     4741 ttttataaat gcgtccgtgg ccgtgggcgg cagcgagatt tggtgcatga aaacaatcgc
     4801 gttgatggcg atcagcagca gcgtcacgaa tggtatcgac cgcctcggcg tcatatcctt
     4861 cagcgggatc atggcttaga ctcgcacgat ggtttggttg ctgcgattgc cgcagcgcgc
     4921 agggatccag gcgcataggc aatttcagat cgtgaggcgc ctcgacggaa attttcaatc
     4981 tcctccaaag ttgcgatgtg cccgcgcgca ccagtcgcgg tacaattcag cgcggccgcc
     5041 gcgcaactga attcaagtat ttcttccggt tgccacccgc gcaccaatcc gaacaaaaac
     5101 gcgccgtgaa aaacgtcgcc cgcgccggtg gtatccgcgg cgcgaatgcg aaatccgggg
     5161 caaagcagaa actgctcacc atcccagatc agcgcaccca tctttccgag tgtcgccccc
     5221 accatgcggc acttgaaccg ccgacaaatt tgcgggagcg ctttcagcaa atccttctcg
     5281 gcagttaatc tttcggcaaa gtttttcgac gcaatcgcaa aatctgtata ttcaagtaac
     5341 gcctcaactc ccggataaca attatcgaag tcgcccacaa ccggtatctc ttcctcgcga
     5401 gcccagcgag ccgcctgcgc cccagcctcg gtgtcatgtc catccacgag aagggcactc
     5461 gcgccagaaa tccattcgcg cctcaaatct tctgtacgta aagcgatact tgaatctcga
     5521 ttccaaagca cggtccgctc gcccgacgct tcgtcaacca gtatgtatga ggattggctt
     5581 gcgcatccgc cggcaatgat ccaacgcgcg tccacgcctt cacggtccat ttcgccaatt
     5641 tgcattttac cggcggcatc gtcgccaact tttcccacgt atcgcgtcga aagtccccag
     5701 cgctggcatg caaccatggc actggcgacc tgtccgccgg ccttcacctc ggacgaacga
     5761 atttcaacct tggaatcgtg cgcagggaag tgcggcagtc ggatgatcgt atccgtggcg
     5821 ttgattccca cgccgacaac gtccacggaa ccgaaggctc tcgctggatt catcttgcga
     5881 ccactttagc cgagcttttg atcttttcaa atgactcgcc gaaaagtccg cgagcgcgtt
     5941 tattaattgc gccggctgat ttgctgtgat agaaaaattc ctccgaaatg tatgcgagtc
     6001 gcgcgcctga taaactactt tacatcgcgg tgccctcgcg tcaggagtcg gcaagccgac
     6061 acttcagatg ccacttcatg aatctgaagc tatcattctt cagcgctacg cgctcggcga
     6121 atccgatcga cttgtcagtt ttttttcgcg caccatgggt aaaatgcgcg gagtggctgg
     6181 cggcgctcgc aagccgaaaa gtcggttcgg cgccacactt gaaaggcttt cttacattcg
     6241 aatctggttt ttcgagcgcg aaacgcgcga actggttcgc atcagccagt gcgaaatgat
     6301 cgaatctttc ctcgacgcgt tcagcgatta tccgtcgagt gtggccctgg ccctgttcag
     6361 cgaaataacg gaaacggttt tgccggatcg ggagccatcg gacgcaaatt ttcgcttgct
     6421 gttgctcgca gcgcaggccg tgaagcgcac cggcaagccg gatttgcctc tggcctactt
     6481 cgcgctgtgg acggtgaaat tgggcggctg gcttccatcg ttgacgcgct gcgctcaatg
     6541 cggcgctgcc ttagatgcag atgcggcggc atattttgtg ccaagcgttt ccgcggctgt
     6601 gtgcgggaaa tgtcgaaaac cggggatgcg gatgctttca ccggcagctc agggcgctgc
     6661 ccgaaaagtc ctggaagagc ggctggatcg cctcaccgaa aaatcggacg cgcccagccc
     6721 gcgcgcagcc agggagctgg cggacttaat gctcgatata atcgagcgcc aaattgatcg
     6781 caaattgaag gctcgcgaac ttctagaatc gcacgcgtaa agcttctagt ttgaaacgtg
     6841 caaagcttct aactcgaaac attaagatga caaaaacgga ctccaaacgc cggggattgg
     6901 ccttccaaga ccttctacta aagctcgaac attattggac cagtcgcgac tgcattttgc
     6961 agcagcccta cgacatcgaa gtgggcgccg ggaccatgca cccggaaact tttctgcgcg
     7021 tcctcgggcc gcagccctac cgcgtggtgt acgcgcagcc gagtcggcgc cccaccgacg
     7081 ggcgctacgg cgaaaaccca aataggttat acaaacacac gcagcttcaa gtgatactta
     7141 aaccatcgcc gtccgacatt caggacctct atttagaaag cctctccgct gtcgggatca
     7201 atctaaaaga gcacgatgtt cgattcgagg aagataattg ggagtcgcct acgctgggtg
     7261 cctggggaat cggttggcaa gtgctgctgg atggacttga gatcacacag ttcacttatt
     7321 ttcagcagtg cggcggaatc gacctcgatc caatttccgt ggagcttact tacggccttg
     7381 atcgtatcgc cgcgtatctg caaggcgtcg acaatgtatt tgacgttcgg tggtcaaaca
     7441 cgatgacata cggacaggtg cggctggcag aggagcagca gttctcagct tacagttttg
     7501 attatgccga tgcggcttcc acaagaaagc aattcgattt gaatgaagct gaggccgggc
     7561 gcctgctttc caagtggggt gctgccagca agaaggatgc gatcgaaacc aagcgctttc
     7621 ctatcttaca ggcctacgac ttgtgcctga agtgctcgca cctcttcaat gtgctcgacg
     7681 cacgaggcgc aatttcggta acagagcgcg ccgggatgat tgggcgcgtg cgaaagctcg
     7741 caacccaggt tgcgcgggcg tggctcgatc aacgggcggc agtggaagct accgccggag
     7801 tcgtcgcgtg aagccgaaag taaaatccag cggcgcgcgc aaatcttcaa gcgatctgaa
     7861 atccacgaaa acttccgccg attttctttt cgagatcggg tgcgaagaaa ttccggcggg
     7921 catgat
//
LOCUS       DATNDT010000166        12088 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3792590, whole genome shotgun sequence.
ACCESSION   DATNDT010000166 DATNDT010000000
VERSION     DATNDT010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12088
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3792590"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="VN788_05505"
     CDS             complement(<1..317)
                     /locus_tag="VN788_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXU20021.1"
                     /translation="MASKVRKAVFPAAGLGTRFLPATKAQPKEMLPLVDKPLIQYGVE
                     EALHSGIQNIFIVTGRGKSSIEDHFDVSFELEHTLEMKNKKDLLAIVRSVSDMINVAY
                     IRQ"
     gene            444..704
                     /locus_tag="VN788_05510"
     CDS             444..704
                     /locus_tag="VN788_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HXU20022.1"
                     /translation="MAYVIAEPCIGTKDTACVDACPVDCIHPKKDEPDFEKHELIYID
                     PVECIDCGACVPVCPVSAIFALDDLPEKWTSFTKMNADYFGR"
     gene            741..1865
                     /gene="tgt"
                     /locus_tag="VN788_05515"
     CDS             741..1865
                     /gene="tgt"
                     /locus_tag="VN788_05515"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HXU20023.1"
                     /translation="MRFEIQAECPATGARAGLLHTAHGVIETPVFMPVGTQATVKGLP
                     QRNLIEDLDARIILANTYHLFLRPGHELIRQLGGLHRFMSWPRALLTDSGGFQVFSLS
                     SLRKITESGVTFRSHLNGDAHLFTPESTVDVQLAFGSDILMALDECLPYPASHESARD
                     AMRRTVRWARVAYSHFQTRKEQAEIASSLFPIVQGSMFRDLREECAGELVGLGANGYA
                     IGGLSVGEPRELSLEVAGYTAPMLPRDRPRYAMGVGMPEELPEYVALGVDMMDCVLPS
                     RNARNGYLFTSGGRIIIKQSRYRDDASPLDPKCSCYTCRTFSRAYLRHLFQAGEILFS
                     TLATLHNLRYYLDTMRGIRQSILLGGFPAFLDSVRSATVE"
     gene            1957..2274
                     /gene="yajC"
                     /locus_tag="VN788_05520"
     CDS             1957..2274
                     /gene="yajC"
                     /locus_tag="VN788_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HXU20024.1"
                     /translation="MISLGFCFVLLQSSSNPIVGFLPLILIVGIFYLLVFMPMQRQKK
                     QQQKMLRELQNGNTVTTTGGIVGTIVSLADDMLVIRVKPDNIKLQVSRSAVSGLVSEE
                     KKV"
     gene            2338..3951
                     /gene="secD"
                     /locus_tag="VN788_05525"
     CDS             2338..3951
                     /gene="secD"
                     /locus_tag="VN788_05525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HXU20025.1"
                     /translation="MKRNIELRTGIIIAVVLICIYGIVGIPKSKAELIANWKKNIHLG
                     LDLRGGSHLVLQVQLQDAFMASADQVMERLKAEAVKQNVTVGSTDRNELASLDDADKI
                     QINIHGVPSNNSSAFRSLVSNEFPDWILTPVNSTDYRMTIKPSSALKLKDDTLTQTMH
                     TIERKINGLGLTESSVQQRGRADAEAELLVQMPGVDDPGRVRQILQTQAMLELYEVKD
                     GPFASREEVLAKHGGVLPLNTKLFPAQARAGNPAGWYLLARSPVIRGNDLRDAHASQG
                     EMGRWETNFVLTQDAARRFEQFTGANIGNRLAIVLDGQVISAPTIQARIGDTGVITGA
                     SSQEEAADLALNLRAGSLPARVVFEDERTVGPSLGNDSIHEGFRAGIFGVLAVVIAML
                     LYYKGAGINATLALILNAVILIAALSYFDATLTLPGIAGIILTIGMAVDSNVLIFERV
                     REELRAGKGTVAALDAGFSKAFLTIIDTHVTTIVSCAFLFLFGTGPVKGFAVTLVIGL
                     FANVFTAVFVSKTIFDWELSRKRVATLSI"
     gene            4043..5248
                     /gene="secF"
                     /locus_tag="VN788_05530"
     CDS             4043..5248
                     /gene="secF"
                     /locus_tag="VN788_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXU20026.1"
                     /translation="MQIFENTNFDFLGKKWPFIILSLILTAAGLVSLALKGGPRYGID
                     FRGGALMYVRFSQKPPVEKIRAALDKKLTVDVQEEVGHNEVIIGTELASNEQLNTARQ
                     IMAQVLESTFGNADGKLDINNTGAQALADRLRDPLQRANVPISEQQLQDLTKNILNYR
                     DTPPRSGLIAKLDDLASVPGVTPQIVNVLKQETWPAQFAVRDTEIVGPKIGADLRHQA
                     ILATLYALGGMLVYVAFRFEWIYGVAAVVAVFHDTIITIGLFSIFDKEISLTVIAALL
                     TLVGYSMNDTIVVFDRIRENLKILRRETLTNLINKSINQTLSRTVLTSGLTFLTAVSL
                     YLFGGQVLNGFSFCLVVGIVIGTYSSIYIASPILVWSQNIAERRKRPSAAPAKTQREE
                     VRRGAAKVK"
     gene            5316..6059
                     /locus_tag="VN788_05535"
     CDS             5316..6059
                     /locus_tag="VN788_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXU20027.1"
                     /translation="MFEQTFVEGTAKTHKSWTVLVSFAGQMVLIGVLVLLPLIYTDVL
                     PKAQLTSFLVAPPPPPPPPPPPPAAPPKVVHVAPRQFDAGKLMAPKVIPKEIATIKDE
                     ALPPPGAVGGVPGGVPGGVAGGVIGGIVGSVPSVAPPPPPPPKAKPVTPSRIRVGGNV
                     QRANLIRQVMPQYPPLAKQARIQGTVRFTAIIGKDGHIQNLQLISGHPLLVAAAEQAV
                     QQWVYRPTELNGEPVEVVTTIDVNFTLSQ"
     gene            6100..6843
                     /locus_tag="VN788_05540"
     CDS             6100..6843
                     /locus_tag="VN788_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXU20028.1"
                     /translation="MFLQLQVLAFMFFQDAGTAPVNFDPRSMWGQMSWLARAVVIVLF
                     FMSAWSIGVMIDRLIAYNSARKQSRAFAPAVAGALREGKLDEAIKIADRYNKSHLAKV
                     VVAGLQEFQAHKLSAEISGEEIEASRRALERAEAIVHAELKRGISSLATIGSTAPFVG
                     LFGTVVGIIHAFTGIATEHATGLGAVAGGISEALVTTAVGLFVAIPAVWMFNYFTNRL
                     EAFDVEMGNSSSELLDYFLKRRERIGAAK"
     gene            6945..7406
                     /locus_tag="VN788_05545"
     CDS             6945..7406
                     /locus_tag="VN788_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU20029.1"
                     /translation="MKKPNAGSTIADINVTPMVDVMLVLLIIFMVITPMLQHGASVDL
                     VKAKNSIEMQAADKEDAVLIAVTRDGKIYLSPGNTQMTADALPSKVKDLLANKLDKEC
                     YIKADARARYEQVENVVDNLRAAGVDQIGLLTEPAQNSNPNNRPSATTVVQ"
     gene            7431..7856
                     /locus_tag="VN788_05550"
     CDS             7431..7856
                     /locus_tag="VN788_05550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU20030.1"
                     /translation="MSMAVGGGGRQVSDINVTPLIDVLLVLLIIFMVITPLTPHGLNA
                     LAPQPPKDKTPPPPDQDRTVVIIIDKDKSMKLNAEPTDMAKLGPRLEDIFKTRADRVV
                     FVKGDPDLEFQTVAQAIDIAKGAGIDKIGLMTPQVEAGK"
     gene            7873..8715
                     /locus_tag="VN788_05555"
     CDS             7873..8715
                     /locus_tag="VN788_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20031.1"
                     /translation="MKKILTAVALACFVLLGTGCNKLKSRDQLNKGVQAFKNAKYNEA
                     VEHFKTAVELDPSNQNARIYLATSYFVQYIPGAASPENDQMAKAATDEFMKALQQDPK
                     DKNALASLASLDYQEAQGIANLDDKLKKLDEAKEWNLKLVQVDPNNKEAYYSLGVIDW
                     LKWYPKWNDARVKLGMKPEDPGPLKDKKVKEELKTQYGSLISDGIEKLQKALSIDPNY
                     ADAMAYMNLLIRERADLQDTQDAYKQDIDTADNWVQKNLETRKILAAKASKSVGGVTP
                     DAAK"
     gene            8776..10962
                     /locus_tag="VN788_05560"
     CDS             8776..10962
                     /locus_tag="VN788_05560"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXU20032.1"
                     /translation="MSPVETLPACSSPADLYAELKEKVQALRPKEDLSALDRAYRLAA
                     DAHKGQCRDDGSPFMLHPLHVAHLLADMHMDLVCLQTGLLHDTVEDTRVGIERIGKEF
                     GEEVERCVDGVTKLKKLEAYSREERQAESVRKMLLATVKDIRVVIVKLADRLHNMRTL
                     GNIQDSERRQRIAQETLDIYAPIAHRLGMGKIRGELEDLSFEYLEPEAWAHLKQEIES
                     KRQQNEQALKRVQHDIEVNLAREGIPATVKGRVKRAYSVYQKLKRQKISLDQVYDLLA
                     LRIVTDSVKNCYAALGVIHNEWHPIPGRIKDFIAIPRTNLYQSLHTSVIGPDGTAFEV
                     QIRTEEMHRVAEEGIAAHWKYKEGKKGPAVDEQRMAWLRQLVDWMQEMKDPGEFMSTL
                     RVDLYTSDDVYAFTPRGRVVTLPRDSTPIDFAYAIHTDVGHACAGARVNGRIVPLRYT
                     LKNGDIVEIITQPGHQPSKDWLPIVKTPRARNKIRHVINASERAKAIDLGEKYLEREA
                     RRVGVQLGRLDKADFERVAADYGVSKMEDLYAALGYGKYSPRQVLEKLTPEHPAEPEP
                     AKAPPSARAEPKDLVIKVSGMDDLLIYRAKCCNPIRGQSIVGFVTRGKGVAVHSVNCT
                     NVQSLMYDPERKIDVEWAGGAEEIFAVKVAIQTDDRPGMLHNVTSLLVNEKSNIRSLE
                     ARSDEKRNGDGAIVDMTIEIKDQRQLSHILAGIRRISGVRDVQRIG"
     gene            10968..11327
                     /locus_tag="VN788_05565"
     CDS             10968..11327
                     /locus_tag="VN788_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXU20033.1"
                     /translation="MAVDPEHIAISKSKGIKIDWKDEHHSEYSLAYLRDECPCASCTG
                     AHGTEPQKSDYSAPPPASAFPMFKPALKMNSVEEVGSYAIRIFWSDGHSTGIYSFDHL
                     RRICPCDKCKAERQIAS"
     gene            complement(11659..>12088)
                     /locus_tag="VN788_05570"
     CDS             complement(11659..>12088)
                     /locus_tag="VN788_05570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20034.1"
                     /translation="GPDARSALLRNFFLSHHCALNSYNADFLEAADTNHLDWRLLPSL
                     AVVESGCVARNGTNNLFGWKSGRARFPSIQAGIRRVAARLATSRIYAGREMRQVLKAY
                     NSGHRRYAARVLAVMNSLSRNRNLIAEDTGKPDFNFADLQ"
BASE COUNT         2678 a         3427 c         3464 g         2519 t
ORIGIN      
        1 ttctgccgga tgtaggcgac gttgatcatg tccgatacgc tgcggacaat cgccagtaga
       61 tccttcttgt tcttcatttc gagcgtgtgt tccaactcga agctcacgtc gaaatgatct
      121 tcaatcgagc tctttccgcg cccagtaaca atgaaaatgt tctgaattcc tgaatgaagc
      181 gcttcttcca cgccatactg gatcagcggc ttgtccacca gcggcagcat ttctttgggt
      241 tgtgccttgg tggcgggcag gaagcgggtg ccgagtcccg ccgctgggaa cacggctttg
      301 cgcactttgg aagccatcag acctattgta tgcggcttgg cccgctgcct ctcgcgagac
      361 ttagaatttt tccattgcta taatacgaat tcatcctgtt tgatccccgt ttgggcaacc
      421 cgcttgggag ttttggagag gtaatggctt acgtaatcgc tgaaccgtgc atcggaacga
      481 aggatacggc ttgtgtggac gcttgtccgg tggattgtat ccatccgaaa aaggacgagc
      541 ccgactttga aaaacacgag ttgatctata tcgatccggt ggaatgtatc gattgcggcg
      601 cttgcgtccc agtgtgccca gtttccgcta ttttcgcgct tgacgacctc ccggagaaat
      661 ggaccagctt cacgaagatg aacgcggact acttcggccg ctaagcatca ccgttcctgg
      721 ctcggcttta cagtagaaac gtgcgttttg aaattcaggc ggagtgtccg gccaccggag
      781 caagagccgg gctgctgcat accgcgcacg gcgttattga aacccccgtc ttcatgccgg
      841 tcggaacgca ggcgactgtc aaaggtctgc cgcagcgaaa cctgattgaa gatctcgacg
      901 cgcgcatcat actcgcgaac acctatcatc tctttctgcg tccaggtcac gaactcatcc
      961 ggcaacttgg cggacttcac cggttcatgt cctggccgcg cgcgcttctg actgattccg
     1021 gaggatttca ggtcttttcg ctaagcagcc tgaggaagat cacggaatct ggcgtaacct
     1081 tccgctctca tctgaatggc gacgcgcacc tgttcacgcc tgaatccacc gttgacgtcc
     1141 aactcgcctt cggtagcgac attctgatgg ccttggacga atgcctccct tatccagcca
     1201 gccatgagtc cgcgcgcgat gccatgcgcc ggacagtgcg ttgggcgcga gtcgcgtaca
     1261 gccattttca aacgcgaaaa gagcaagccg agatcgcctc ctctctcttc ccaatcgtgc
     1321 aaggctctat gtttcgcgac ttgcgcgagg agtgtgccgg agaactagtg gggcttggcg
     1381 ctaacggtta cgcgatcggc ggcctatccg tcggagagcc gcgcgagctg agtctggaag
     1441 tcgccgggta caccgcgcct atgctgccgc gtgatcggcc gcgctatgcg atgggcgttg
     1501 gaatgccgga ggaactccct gaatatgtgg ccctcggcgt ggacatgatg gattgcgtct
     1561 tgccgtcgcg caatgcccgc aatggatatc tgtttacatc cggaggccgc atcattatta
     1621 agcagtcccg gtatcgcgac gacgcctcgc cgctcgatcc gaaatgctct tgttacacct
     1681 gccgtacctt ctcccgggcc tacctgcgtc atttatttca ggctggggaa atcctgtttt
     1741 cgacgctggc gacactgcac aatctccggt actaccttga caccatgcgc ggaatcaggc
     1801 agtctattct ccttggggga tttcctgcct tcctggactc cgtacgctcc gcgacggtag
     1861 aatgaaaggc aagttttatc ttcccgcgac gctcacggtt gtcctttgtt cccgaaagag
     1921 cctttgagga gttgaagctg agatagtttg accgcgatga tatcacttgg tttctgtttc
     1981 gtcctgctac aatcgtcctc aaacccgatt gtcggttttc ttccgctgat tctcattgtc
     2041 ggcatttttt atttgctggt cttcatgccg atgcagcgcc agaagaagca gcaacaaaag
     2101 atgctgcgcg aattgcagaa cgggaatacc gtcaccacca cgggcggcat cgtgggaacc
     2161 atcgtgagcc tcgcggacga tatgctggtg atccgggtga agccggacaa tatcaagctg
     2221 caagtcagta ggagtgcggt gtccgggcta gtgtctgaag agaaaaaggt atagaacagt
     2281 acagtaagga agtaaactgg gcttgccgcc gcctgcggcg cgcgcccggc tgaacacatg
     2341 aaacgcaaca ttgaactcag aacgggcatt atcattgccg ttgtcctcat ctgcatctac
     2401 gggatcgtcg ggattccaaa gtcgaaagcg gagttgatcg ccaactggaa gaagaacatc
     2461 caccttgggc tggatttgcg gggcggcagc cacttggttt tacaggtcca gcttcaggac
     2521 gcgttcatgg cgtcggcgga tcaggtgatg gagcggctga aggcggaggc cgtaaaacag
     2581 aacgtcacag tcggttcgac cgatcgtaac gaactcgcct cgctcgacga tgccgacaag
     2641 atccagatca acattcacgg tgtaccctcc aataattcct cggcgttccg gagtctcgtg
     2701 tccaacgagt tcccggattg gattctgact ccggtgaatt caaccgacta ccggatgacg
     2761 atcaagcctt cttcagcatt gaagctgaaa gacgatacgc tgacgcaaac gatgcacacg
     2821 atcgagcgga agattaacgg tctcggcctc actgaatctt cggtgcagca gcgcggccgc
     2881 gccgatgcgg aagcggagct gctggtgcaa atgccaggcg ttgacgatcc tggccgcgtc
     2941 agacagatcc tgcaaacaca ggcgatgctg gagctgtatg aagtgaagga tgggcctttc
     3001 gcttcgcggg aagaagtgct ggccaagcac ggcggcgttc ttccgctgaa tacgaaactg
     3061 ttcccggccc aagctcgcgc gggcaatccc gctggttggt acttgttggc cagaagtccg
     3121 gtaatccgcg ggaacgatct gcgcgacgcg cacgcgagcc agggcgagat gggccgctgg
     3181 gaaactaact ttgtcctcac acaggatgcc gcccggcgct ttgagcagtt caccggcgcg
     3241 aatatcggca accggcttgc catcgtgctt gacggacagg tgatcagcgc gcccaccatt
     3301 caggcccgca tcggagacac aggcgtgatt accggcgctt ccagccagga ggaagcggcc
     3361 gatctggctt tgaatcttcg cgccggctca ttgcccgcac gcgtggtatt tgaagacgag
     3421 cgcacggttg gcccctcgct tggaaacgac tcgattcacg agggattccg cgcgggtatc
     3481 ttcggcgttc tcgcggttgt gatcgcgatg ctgctctact acaagggagc cggtatcaat
     3541 gcgacgctcg cgctgatttt gaatgccgtc atcttgatcg ccgccctgag ctatttcgac
     3601 gcgacgctta ctcttcccgg tatcgccggc attattttga cgatcggcat ggcggtggac
     3661 agtaatgttc tcatctttga gcgtgtgcgc gaggagttgc gcgccggcaa ggggaccgta
     3721 gccgcgctcg atgccggttt cagtaaggcg tttctaacaa ttatcgatac gcacgtgacg
     3781 acaatcgttt cgtgcgcttt cctcttcctc tttggaaccg gcccggtgaa gggcttcgcc
     3841 gtcactctgg tgatcggcct cttcgccaac gttttcaccg cggtctttgt ttcaaagacc
     3901 atcttcgatt gggagttatc gcgcaagcgc gtcgcaactt tgagtatcta gcaaagtttg
     3961 tgtaaacttt tggaattaac aacgggaact ctttcggaca tgccggtgtc taacgctatc
     4021 gaatgccggc cctttgtgct tcatgcaaat ttttgagaat acaaatttcg acttcctggg
     4081 caagaaatgg ccattcatca tcctgtccct cattcttacg gcggctgggc tggtgagttt
     4141 ggcgctgaag ggcggcccgc gttacggcat cgatttccgc gggggagcgc tgatgtacgt
     4201 gcgtttctcg caaaaacctc cggtggaaaa gattcgcgcc gcgctcgaca agaaactaac
     4261 tgtggacgta caggaagagg tcggccacaa cgaagtgatc atcgggaccg aattggcgag
     4321 caacgagcaa ttgaacaccg cccggcagat catggcgcag gttcttgaat cgaccttcgg
     4381 taatgcggat ggcaagctgg atatcaacaa caccggcgcg caagccttgg ccgaccggct
     4441 ccgcgatccg ttgcaaaggg cgaacgtccc tatttcggag cagcagcttc aggatctgac
     4501 gaagaacatc ttgaattatc gcgacacccc gccgcgctcc ggcctgatcg cgaaactaga
     4561 cgatctcgcc tccgttccgg gcgtgactcc gcagatcgtc aatgtgctga agcaggagac
     4621 gtggcccgct cagtttgccg ttcgcgatac ggaaatcgtc ggcccgaaga tcggagccga
     4681 tctgcggcac caggccatcc tcgcgaccct gtacgcgctc ggcggaatgt tggtttacgt
     4741 ggcgttccgt tttgagtgga tctatggcgt ggcggccgtg gtagccgtat tccacgacac
     4801 cattatcacg atcgggctgt tctcgatctt cgacaaggag atctcgctca ccgtgatcgc
     4861 cgcgctgctg acgctagtcg gttattcgat gaacgatacc atcgtggtgt tcgaccggat
     4921 cagggagaat cttaagattc ttcgccggga gacgcttacc aacttaatta acaagagcat
     4981 caaccagacg ctgagccgga ctgtcctcac ctcaggattg actttcctga ccgccgtgtc
     5041 tctatatctg ttcggcggcc aagtactcaa cggattttca ttctgcctgg ttgttggtat
     5101 agtcattgga acgtattcgt cgatttatat cgccagtcca attctggttt ggtcgcagaa
     5161 cattgcggaa aggcgcaagc ggccctcggc cgcgccggcc aagacgcagc gggaagaagt
     5221 tcgaagagga gcagcgaaag taaagtagcg gccaaacaac actcgcgcca tgtggcgcga
     5281 aacccggccg cggaagtgaa aggggaacag agtttatgtt cgagcagact tttgtagagg
     5341 gtacagccaa aacccataag agctggacag tgctggtgtc cttcgcaggc cagatggttc
     5401 taattggagt cctggtattg cttccattga tctacaccga cgtcctgccg aaggcgcaat
     5461 tgaccagttt cctggtggcc ccgccaccgc cgccaccacc accgcctccg cccccggcag
     5521 cgccgccgaa ggtggttcat gtggcgcctc gccagttcga cgccggaaaa ttgatggcgc
     5581 cgaaagtaat tcccaaagag atcgcgacta ttaaggacga ggctcttcca cctcccggcg
     5641 ccgtcggtgg cgtccccggc ggagttccag gtggagtcgc cggaggtgtg attggaggaa
     5701 tcgtgggatc cgtgccgtcc gtggcgcccc cgccgcctcc gccgcccaag gcgaagcctg
     5761 tcacgccttc ccgaattcgt gtcggcggca atgttcaaag ggcgaatctg attcgtcagg
     5821 tgatgccgca atacccgccg ctcgccaagc aggcgcgcat tcagggaacg gtccgcttca
     5881 ccgcgatcat cggtaaagac ggtcacattc agaacctcca actcatcagc ggccatcccc
     5941 tgctggtggc ggccgccgag caagccgtgc agcagtgggt ctaccggcca actgagctga
     6001 acggtgagcc agtggaagtc gtgaccacga ttgatgtaaa tttcacgtta agtcagtagt
     6061 tcgacagtaa agttcaaatt caactcgcag gagagaacca tgtttttaca attgcaggtc
     6121 ttggcgttta tgttctttca agacgctgga acggcgcccg tcaacttcga ccccaggtcg
     6181 atgtggggtc agatgagctg gctcgccagg gcggtcgtga tcgtgctgtt cttcatgtcg
     6241 gcctggtcaa tcggcgtcat gatcgaccgc ttgatcgcct ataacagcgc gcgcaagcag
     6301 tcgcgcgctt ttgcccccgc cgttgccggc gcccttcgcg aaggcaaact cgacgaggcc
     6361 atcaaaattg ccgaccgtta caacaagagc catctggcga aggttgtcgt ggccggattg
     6421 caggagtttc aggcgcataa actgagcgcg gaaatttccg gggaagagat cgaggcatcg
     6481 cgccgcgctc tcgaacgcgc cgaagcgatc gtgcacgcgg aactcaagcg cggcatcagc
     6541 agtttggcga ccatcggctc cacggccccg ttcgtcggac tgttcggaac ggtcgtcggt
     6601 atcattcacg cgttcaccgg cattgccact gagcacgcga ccggtctggg cgcggtcgcc
     6661 ggcggcattt ccgaagcgct ggtcaccacc gccgtcggtc tgttcgtcgc catcccggcg
     6721 gtctggatgt tcaactactt taccaaccgc ctcgaagcgt tcgacgtgga aatgggcaac
     6781 tcgagttcgg agctgctcga ttatttcctg aagcgccgtg agcgtatcgg agcagcaaaa
     6841 taacaaaggt gcaggcgagt tgaataaggg tgaagcgcgg ccgggtattc tggccgcgct
     6901 tctctcgaat cgactgaacc ggaagtaaac ccaggagtaa aacagtgaaa aagcctaacg
     6961 cgggttcaac gatcgccgac atcaacgtca ctcccatggt cgacgtaatg ctcgtcctgc
     7021 tgatcatctt catggttatc acgcccatgc tgcaacatgg cgcgagcgtg gatcttgtga
     7081 aagccaagaa ctccatcgag atgcaggcgg cggacaaaga agatgccgtc ctcatcgccg
     7141 ttaccaggga cggaaagatt tatctgagtc ccggaaacac gcagatgact gccgacgcgc
     7201 ttccgagcaa agtaaaagat ctgttggcga acaagttgga caaagaatgc tacattaagg
     7261 ccgatgcccg cgcccgttac gaacaggtgg aaaacgtggt ggacaatctc cgggccgccg
     7321 gcgtggacca gatcgggcta ttgaccgagc cggcccaaaa ttcaaacccg aacaatcgac
     7381 cgtcggcaac caccgtggtc cagtaataga atttttagga gcctaatttt atgtcaatgg
     7441 cagtaggtgg cggcggacgc caagtatcgg acatcaatgt cactccgctg atcgatgtgt
     7501 tgctggtgct gctgatcatc tttatggtca ttacgccgct cactccgcac gggttgaacg
     7561 ctttggcgcc tcagcctccc aaggataaga cccctcctcc tccggatcag gatcgcacgg
     7621 ttgtcattat catcgacaag gacaagagca tgaaactcaa cgccgagccg accgatatgg
     7681 cgaaactcgg gccgcggctc gaagacattt tcaaaaccag agcggaccgc gtggtgttcg
     7741 tgaaaggcga tccggacctt gagttccaga ccgtggcaca ggccatcgac atagccaaag
     7801 gcgctggcat cgacaagatc ggcctgatga ctccgcaagt tgaagcgggc aaataggaga
     7861 accctggcac acatgaagaa gatattaacg gccgttgccc tggcgtgttt cgttcttctc
     7921 gggacgggct gcaacaagct gaaatcgcgc gaccaattaa acaagggcgt gcaggcgttc
     7981 aagaacgcca agtacaacga agccgttgag cacttcaaga cggccgtgga attggacccg
     8041 agcaaccaga acgcgcgtat ctatctggcg acgtcatatt tcgttcagta catacctggg
     8101 gctgcgtctc ctgagaacga ccagatggcg aaagcggcaa ctgacgagtt catgaaggct
     8161 ctccaacagg atcccaagga caagaatgcg ctcgcgtcgc ttgcctctct cgattatcag
     8221 gaggcgcagg gcatcgccaa tctcgacgac aagctgaaga aattggacga ggccaaggag
     8281 tggaacctca agctggttca agtcgatcct aacaacaaag aggcgtacta cagcctcggc
     8341 gtaatcgatt ggctgaagtg gtaccccaag tggaacgacg cccgcgtaaa gctcgggatg
     8401 aagccggaag atcccgggcc gctgaaggac aagaaggtca aggaagagct gaagacgcag
     8461 tacggatcgc tcatctccga cggtatcgag aagcttcaga aagcgcttag catcgatccg
     8521 aattatgccg acgcgatggc gtatatgaac cttctgattc gcgagcgtgc cgatctgcag
     8581 gatactcagg atgcgtacaa gcaggacatt gatacggcgg ataattgggt gcagaagaat
     8641 ctcgaaacca ggaagatttt ggctgccaag gcttccaagt ccgtgggcgg cgtgacgccc
     8701 gatgccgcca agtagtttcg tcgaaaaatc ccggttccgg tcgataatgg ctggaaccgg
     8761 gtaagctgta attagatgtc ccctgttgaa accctgcctg cctgtagttc ccccgccgat
     8821 ctctatgccg aactaaagga aaaggtacag gctcttcgcc cgaaggaaga tctttccgcc
     8881 ctggaccgcg cctatcggct tgccgcggat gctcacaagg gccagtgccg tgatgatggc
     8941 agcccgttca tgcttcatcc gctgcacgtt gcccatcttc ttgctgatat gcacatggat
     9001 ctggtctgcc ttcagacggg gttgctgcac gacaccgtgg aggacaccag ggttggtatc
     9061 gagcggattg gaaaagaatt cggcgaggag gtcgagcgtt gcgtcgacgg cgtgaccaag
     9121 ctcaagaagc tggaagccta ctcccgcgaa gagcgtcagg ccgagagtgt ccgcaagatg
     9181 ctactggcca cggtcaagga cattcgcgtc gtcattgtga agctggccga ccggctccac
     9241 aatatgcgaa ccctcggcaa tatccaggat tccgagcgcc gccagcgcat cgcccaggag
     9301 acgctcgata tttacgctcc catcgctcat cgcctcggaa tgggcaaaat acgcggcgag
     9361 ctcgaggatc tttctttcga ataccttgag ccggaagcct gggcgcatct caagcaggag
     9421 atcgaatcga agcgccagca gaatgaacaa gccctcaaac gtgtccagca cgatattgag
     9481 gtgaacttgg cgcgcgaagg aatccccgct acggtgaagg gccgcgttaa acgcgcctat
     9541 tcggtctatc aaaaactcaa gcggcagaaa atcagtctcg atcaggttta cgatctgctc
     9601 gcgctgcgca tcgtcaccga ttccgtgaag aactgctatg ccgcgctcgg agtgattcac
     9661 aacgagtggc atcccattcc ggggcgcatc aaggatttca tcgcgattcc gcggaccaat
     9721 ctttatcagt cgcttcatac ctccgtcatc ggcccggatg gcactgcgtt cgaggtccaa
     9781 atccgcaccg aagaaatgca ccgtgtcgcg gaggaaggta tcgcggcgca ctggaaatat
     9841 aaagaaggca aaaaaggtcc ggcggttgac gagcagcgga tggcgtggct gcgccagctt
     9901 gtcgattgga tgcaggaaat gaaggacccg ggcgagttca tgtccactct ccgggtggac
     9961 ctgtacacct ccgacgatgt ctacgccttc acgcctcgcg ggcgcgtggt cacgctgccg
    10021 cgcgactcaa cgcccatcga cttcgcttac gccatccaca ccgacgtcgg ccacgcctgc
    10081 gccggcgcga gagtgaacgg ccgcattgtc ccgctgcgtt acacgctgaa gaacggcgac
    10141 attgtcgaga tcatcacgca gcccggccac cagccgagta aagactggct gcccattgtt
    10201 aagaccccgc gagcccgcaa caagatccgg cacgttatca atgcaagtga gcgagccaag
    10261 gccatcgacc tcggcgagaa gtatctcgag cgcgaagccc gccgcgtggg cgtgcaattg
    10321 ggacgccttg acaaggctga tttcgagcgc gtcgccgccg attacggcgt cagcaagatg
    10381 gaagacttgt acgccgcgct ggggtacgga aagtattcgc cgcgccaagt ccttgaaaaa
    10441 ctgacgccgg aacatccagc cgaaccggag ccggcgaaag cgcctccgtc cgcgcgtgcc
    10501 gagccgaagg accttgtcat caaagtcagc ggcatggacg acctgctgat ctatcgcgcg
    10561 aagtgttgca atccgattcg cggtcaatcg atcgtcggct tcgtcacgcg cggaaagggc
    10621 gtggccgtcc attccgtcaa ttgcacgaac gtacagagtt tgatgtacga tcccgagcgg
    10681 aagatcgacg tcgaatgggc cggtggagcc gaggagatct tcgccgtcaa agtggccata
    10741 caaaccgacg accgccccgg tatgctgcat aatgtgacgt cgttgctggt gaatgagaag
    10801 agtaatatcc gcagcctcga agcccgctcc gacgaaaaac gcaatggcga cggagccatc
    10861 gtcgacatga cgattgagat caaggaccag cgccagttga gccacatctt agcgggaatc
    10921 cgccggattt ccggtgtgcg cgatgtgcag agaattggat agaaattatg gccgttgacc
    10981 ccgaacacat cgcgatctcg aaatcgaaag gcatcaaaat cgactggaag gacgaacatc
    11041 acagcgaata ctcgctcgcc tacttgcgcg atgagtgccc gtgtgcaagc tgcaccggcg
    11101 cgcacggaac tgagcctcag aagagcgatt attccgcgcc gccgcccgcg agcgccttcc
    11161 cgatgttcaa gccggctctc aaaatgaaca gcgtggaaga ggtcggaagt tacgcgatcc
    11221 gtattttctg gagcgatggc cacagcaccg gaatctattc gttcgatcat ctccgccgca
    11281 tctgcccgtg tgacaagtgc aaagcggaac gccagatcgc cagttagcaa tcgatgggcg
    11341 gctaattact ttgatcgcgg ctctttcccg ggcgtatgtg gctctccctg aactaacggt
    11401 gcgcctccac caagacgaat ggctggttcc cgatgtggtg atcgcaaaac gcggcacgct
    11461 ggagcggccc tatccaacga aaccagttca cgtctgcatc gaaatccttc tctggaggac
    11521 aggttttcca aggtactcac gaagctcgaa gattacttcg cgtggggcgt tcctttttgc
    11581 tggattgtcg atcaagtgaa acaaaaggcc tggagcgtcg agccggatgg aaggcccaat
    11641 gaggttccag ccgacggact attgcaggtc ggcgaaattg aaatcgggct ttccggtatc
    11701 ttcagcgatg agattccggt tccgcgatag tgaattcatc accgcgagca cccgggccgc
    11761 ataacggcgg tggccggaat tataggcttt cagcacctgc cgcatctccc gtcccgcgta
    11821 tatccgcgac gtcgccagcc tcgccgcaac ccgccgaata ccggcttgga tcgatggaaa
    11881 gcgcgctctc cccgacttcc acccaaagag attattcgtg ccgttgcgcg cgacgcagcc
    11941 cgattcgacg acagccagac tcggcagcag acgccagtcg aggtgattcg tatccgccgc
    12001 ttccagaaaa tcggcgttgt aagaattgag agcgcaatga tgactcagaa aaaaattgcg
    12061 caacagcgcc gatcgcgcat cgggccca
//
LOCUS       DATNDT010000167         5755 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3486951, whole genome shotgun sequence.
ACCESSION   DATNDT010000167 DATNDT010000000
VERSION     DATNDT010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5755
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3486951"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VN788_05575"
     CDS             <1..193
                     /locus_tag="VN788_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXU20035.1"
                     /translation="LLEQRRPRSIRIAALLDKPDRRQRPVHVDYVGFEIPDKFVVGYG
                     LDYAEDYRALRDICILEEG"
     gene            212..1093
                     /locus_tag="VN788_05580"
     CDS             212..1093
                     /locus_tag="VN788_05580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20036.1"
                     /translation="MTKEILEELLRQENSWAGRSTIILVIGILGEYVLPLFFNENNER
                     RQSRWKRVLSIGCGILVVAGISGEYHFGSRISQTANQIQILSDQEVSKAGERASNADR
                     EAARARRDAETTRMSGERLQLDVANANARAERERLARVKIEALLGARHLDRYQKEQIA
                     SRLARYSGIAVDVFVLDQEGDPAEEEAINFGMDIVDALGKPMKVSGYAGSHCLSSSVI
                     GVFISAPQDLSKERYAAGDIAQALKAAGVDVVPYVGTPIAVPCSMFSNLSVQQNSSTL
                     AAKIEVIIGKRPTPILK"
     gene            complement(1115..1348)
                     /locus_tag="VN788_05585"
     CDS             complement(1115..1348)
                     /locus_tag="VN788_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HXU20037.1"
                     /translation="MPATKVTVFNNGPVRIEGDFAIYDAEGKEYGLAGRTAISLCRCG
                     FSDKKPFCDGHHGVQGFESQCQAHELPPPKVKV"
     gene            1373..2497
                     /locus_tag="VN788_05590"
     CDS             1373..2497
                     /locus_tag="VN788_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HXU20038.1"
                     /translation="MIQFVAENDRDLFLEAMRFAQHQVRALVQKDPDFYPMYTVNGRW
                     RHKGPAWTNWCDGFLPGMMWIFLKRVEENSPEALFWREQAERYTKKLEHRKHDRDIHD
                     LGFLFLSTYYRWHQFTREPKLKDVLIEAGKTLALRFQEKGQYLRSFVADNSLFIDIMM
                     NVGLIFYAARETGDRNLRNIAIRHSLTTRRFLVRGDGSTAHEGIFDIESGEFLRQTTQ
                     QGYRGDSCWSRGLAWAIYGFTVAYEHSRDPRFLATAEACSDYFLTHCGADGVPPWDFG
                     APTNRTLLDTSAAAIAASGFLRLCQSITDPAKGHFYWSAAAVILRNLCQKYLAKNDKK
                     WEGILKGGVYHIHKELGVDESVMWGEYFFVEALEQVLRQL"
     gene            complement(2522..3313)
                     /locus_tag="VN788_05595"
     CDS             complement(2522..3313)
                     /locus_tag="VN788_05595"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXU20039.1"
                     /translation="MVLLHAGALAALFMFSWWTFAAAVFLYWMTTGLGISMGYHRLHT
                     HRSYKVPLALEYFFAICGALTLEGGPISWVATHRIHHQKSDRPGDPHSPRDGGWWAHA
                     GWILLGETNHNNTRAMAKYAPDLAKHRFYVWLNNYHWTPTIALAVLLFAIGGLPLMLW
                     GVCVRVVFGLHATWLVNSATHMWGRRRFATRDDSRNNWWVALITFGEGWHNNHHAFPT
                     SARHGLAWYEFDPTFIQITILKYFGIAKSICVAKVNSALAKREAA"
     gene            3704..5104
                     /locus_tag="VN788_05600"
     CDS             3704..5104
                     /locus_tag="VN788_05600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU20040.1"
                     /translation="MKITVVIAAYNEPENIGLLTARLIAVLDDVYHSQWELIYVIDGT
                     DGTLAAAQSFAARRPEIRILHNPYPRGLGSAFRKGFEAIAADTTIVVTMDADLNHQPE
                     EIPKLVRALFGRGADIVVGSRKVSGSVTKGAPFWKRALSDMVNRLMRRMMRMPVSDQT
                     SGYRAYRYEALRKIPFYNTGFAFLPEILMRAHSVGLRIVEEPIQFVFRTVGESKMKLL
                     PTARSYFSLLASSFSRRSPPNGSPQTFGGREWDRHWLSLGNGRSRSLFGLGSSAVRRF
                     IFQPAVRFFLDKYFSAEGIFFEMGCGTGESSAAVPRRSRHFVGLDFSAVALAQSRIAG
                     PFDSRVCADICRLPLQPASIDGIWNLGVMEHFSTPELMQALREFRGVLKPGGIVVLFW
                     PAEANASRWVLAPVEWLRSTIAGKQFRFFPDEISRLKSRGEGADILKSAGFDPLEVDF
                     SIRTFFIHMVVIGRKP"
     gene            5193..>5755
                     /locus_tag="VN788_05605"
     CDS             5193..>5755
                     /locus_tag="VN788_05605"
                     /inference="COORDINATES: protein motif:HMM:NF014518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar cap protein FliD N-terminal
                     domain-containing protein"
                     /protein_id="HXU20041.1"
                     /translation="MGTTPSSTSSLFAPQVFSGVSQFSSDFQSILQRAVSIAELPIQT
                     LQNQQSDLLQQKQLLIGLNTAAASLGSSLGALGSLGANQGLSAASSNSSTVSVTNSGA
                     TSPATYTISNITSVAAAAAETSVSGFADSTSAHVSATGIMKLVVGSSTYAINLASGQN
                     NLNGLADAINSLGAGVTASVLTTGTGGT"
BASE COUNT         1228 a         1671 c         1603 g         1253 t
ORIGIN      
        1 cctgcttgag cagcggcgtc cgcgatcgat ccgcatcgcc gcgttgctcg ataagcccga
       61 ccgccgccag cgcccggtcc acgtggatta cgtgggcttc gaaatccccg acaaattcgt
      121 cgtcggctac ggactcgatt atgcggagga ttaccgggcg ctgcgcgata tctgcattct
      181 cgaagagggt tagtgggttc tggtgggata aatgaccaag gaaatcctag aagagcttct
      241 cagacaggaa aactcatggg ctggacgatc cacgatcata cttgttatcg gcattctcgg
      301 agagtacgtc ctaccactgt ttttcaacga gaacaacgaa aggagacaat cgcgctggaa
      361 gagggttttg agcattggat gcggaatatt agttgttgcc ggtatcagtg gagagtacca
      421 cttcggttca cgaatctcgc aaacggctaa ccaaatccag attctatctg accaggaggt
      481 ttccaaagcg ggagaacgtg cgagcaatgc cgatagggaa gcggcgcggg cgcgaaggga
      541 tgcggaaaca acgcgaatga gtggagagcg ccttcagctt gatgtggcca atgctaatgc
      601 acgcgcagaa agggaacggc ttgcgcgagt aaaaatcgaa gccctccttg gcgcaagaca
      661 tctcgaccga taccaaaagg agcaaattgc ctcaagactg gcacgttact ccggaatcgc
      721 tgttgacgtg ttcgtattgg accaagaagg cgatcccgcc gaggaggaag ctataaattt
      781 cggcatggac atagtggacg ctcttgggaa gcctatgaag gtatctggat atgccggttc
      841 acactgtcta agttcttcag tgatcggagt atttattagt gctccacagg atctatcgaa
      901 agaacgatat gccgccggag atattgccca agccctaaag gcagctggcg ttgacgtcgt
      961 tccatatgtc ggaacaccaa ttgcggtgcc atgcagtatg ttttctaact tatccgtgca
     1021 gcaaaacagt agcacgctag cggccaaaat tgaagtcatt atcggcaaga ggcccacgcc
     1081 gatcttaaag taacgttgaa ccgcgcggcc tacctcacac cttcactttc ggcggcggca
     1141 gttcgtgcgc ctggcactgc gattcaaagc cctgcacgcc atgatgtccg tcgcagaacg
     1201 gcttcttgtc tgaaaatccg cagcggcata gcgaaattgc cgtgcgtccg gcaaggccgt
     1261 actcttttcc ttcggcatcg taaatggcaa aatcgccttc aattcgaacg ggtccattgt
     1321 tgaacacagt gaccttggta gctggcatca gtcagaatgg taccatcgcc aagtgatcca
     1381 atttgttgct gaaaacgacc gcgacctatt tctggaagcg atgcggttcg cccagcacca
     1441 ggtccgggcg ctggtccaga aggacccgga tttctatccg atgtacacgg taaatggccg
     1501 ctggcggcac aaaggccccg cgtggaccaa ctggtgcgac ggcttcctcc ctggcatgat
     1561 gtggattttc ctcaagcgcg tcgaggagaa cagccccgag gcccttttct ggagagagca
     1621 ggccgagcgc tacaccaaga aactcgagca tcgaaagcac gaccgcgaca tacacgacct
     1681 cgggttcctg ttcctctcga cgtactatcg ctggcatcag ttcacgcgcg agccaaagct
     1741 gaaggacgtg ctgatcgaag ccggcaaaac gctggctctc cgcttccagg aaaagggcca
     1801 atacctgcgc tcgttcgttg ccgacaattc actgttcatc gacatcatga tgaacgtcgg
     1861 cctgattttc tatgcggcgc gcgagaccgg cgatcggaat ctgcgtaaca tcgcgatccg
     1921 gcattctctc accacgcggc gatttctggt ccgtggcgat ggatcgacgg cgcacgaagg
     1981 catcttcgac atcgaaagcg gcgagtttct ccggcaaacc acgcaacagg gctatcgcgg
     2041 tgattcctgc tggtcgcgag ggcttgcctg ggccatctac ggcttcaccg tcgcctatga
     2101 gcatagccgt gatccgcgct tcctcgccac cgccgaagcc tgttcagatt acttcctgac
     2161 gcattgcggg gccgacggcg ttcccccgtg ggatttcgga gcgcccacga acaggacgct
     2221 gttggacact tccgccgcgg ccatcgccgc gtccggcttc cttcggcttt gccagtccat
     2281 caccgacccg gcgaagggcc atttctattg gtccgcggcc gctgtcatcc tccgcaattt
     2341 gtgccagaaa tatctagcca aaaatgacaa gaaatgggaa ggcattctca aaggcggcgt
     2401 ttatcacatc cacaaagaac tcggcgtaga tgaatccgtc atgtggggcg agtacttctt
     2461 tgttgaagcg ctggagcagg ttctgcgaca gttgtagccg gaagcgggtg tgctgggcgt
     2521 cttatgccgc ttcgcgtttc gccagcgcgc tatttacctt cgccacgcag atcgacttcg
     2581 cgatcccgaa gtacttcaga atcgttatct gaatgaacgt cggatcgaac tcgtaccatg
     2641 ctaggccatg ccgcgcggaa gtcggaaacg catggtggtt gttatgccat ccctcgccaa
     2701 acgtgatcag cgcgacccac cagttgttgc gcgaatcgtc ccgggtggca aaccggcggc
     2761 ggccccacat atgagtcgcg gagttgacca gccaagtggc gtgaagcccg aaaacgacgc
     2821 gcacgcacac gccccagagc atcaagggaa gccccccgat cgcaaacagc agcacggcga
     2881 gcgcaattgt cggagtccaa tgataattgt tcagccagac ataaaaccgg tgtttcgcga
     2941 ggtcgggcgc gtacttcgcc atcgcgcgcg tattgttatg attggtctct ccaagtagaa
     3001 tccaaccggc atgagcccac catccgccgt cgcgcggcga gtgcggatcg ccgggccggt
     3061 cggacttctg gtgatgaatc cggtgcgttg caacccagga aatcgggccg ccttcgagcg
     3121 tcaacgcgcc gcaaatcgca aagaaatatt caagcgccag gggaacttta taagaccggt
     3181 gcgtgtgcag gcgatggtag cccatgctga tgccgaggcc tgtagtcatc cagtagagaa
     3241 aaacagcggc ggcgaaggtc caccaactga acatgaaaag cgcggcgagt gcgccggcat
     3301 gaagcagaac catgacgatc gcgatgggcc aattgagccc ctcatggtcg cccctttcaa
     3361 aattggcgga gttgatcgcg ccggggttgt gggtggacgc atctcgcggc ccgttcagga
     3421 ccatcaattc agcaacggct ttatcggagg ttgcaggcgc cggacatctt gaaaatgccg
     3481 attggttgcc ggcaggcgtg gaattattca ttgtacagag atctactctt ccatcttaga
     3541 ccttcgcgga gacatccggc aaaacaacaa taagaagggg ttctgtttcg attttccgcc
     3601 gcgcgccgtt tcgcgagtct gccattagat tattcttatg gcaggttccc ggaaacacct
     3661 ggcatactga cgcgccgctt gccatgctcc gccgtcccat ccgatgaaga tcaccgtagt
     3721 aatagccgca tacaatgaac cggaaaatat tggccttctg accgcccgtt tgatagccgt
     3781 tcttgacgat gtctaccatt cgcaatggga actgatttac gtaatcgacg ggaccgacgg
     3841 gaccctcgct gccgcgcaat ctttcgccgc gcggcgccct gagatccgga tactccacaa
     3901 cccctatcct cgcggtctcg gaagcgcctt tcgtaaaggc ttcgaggcga tcgccgccga
     3961 tacaaccatc gtcgtcacca tggatgccga cctcaatcat cagcccgaag agatccccaa
     4021 gctggtgcgc gctctgttcg ggcgaggcgc ggatatcgtc gtgggttcgc gaaaggtgag
     4081 cggcagcgta accaagggag ctcccttctg gaaacgagca ctcagcgaca tggtaaaccg
     4141 cctgatgcgc cgcatgatga ggatgccagt ttccgaccaa acttccggtt accgcgccta
     4201 ccgctatgaa gctttgcgca aaatcccatt ctataatacc ggtttcgcgt ttctgcccga
     4261 gatcctgatg cgcgcccatt ccgttggcct ccgcatcgtc gaggagccga ttcagttcgt
     4321 cttcaggacc gtcggcgagt ccaagatgaa gttgcttccc accgcacgca gttatttctc
     4381 ccttctcgcc tcaagctttt cccggcgttc gccgccgaac ggtagccctc aaacattcgg
     4441 tggacgggaa tgggaccgtc actggctttc gctcggcaac ggaagatcgc gttctctgtt
     4501 cggcctggga tcgagcgccg tgcgccgctt cattttccaa cctgccgtcc gtttcttcct
     4561 cgacaaatac ttttccgcgg aaggtatttt tttcgagatg ggctgcggga ccggagagtc
     4621 gtctgcggca gttcctcggc ggtcgaggca ttttgtcgga ctcgattttt ccgctgttgc
     4681 tctggcgcaa tcgcggatcg cgggaccgtt tgactcgcgc gtttgcgccg acatttgccg
     4741 gcttccgctc cagcccgcat cgatcgatgg aatctggaat ctcggcgtaa tggagcattt
     4801 ctccacccct gagctgatgc aggccctccg ggaatttcgc ggagtgctca aacctggagg
     4861 aattgtcgtt ctgttctggc ctgccgaagc gaacgcctcc cgttgggtgc tggccccggt
     4921 ggaatggctt cgctccacga tcgccggcaa gcaatttcga tttttccccg acgagatcag
     4981 ccgcctcaaa tcgcggggag aaggcgccga tattcttaaa agtgcggggt tcgaccccct
     5041 tgaggtggat ttttccatcc gcactttctt tattcacatg gtggttatcg gccggaagcc
     5101 gtaaaaagat tagtggcgaa gttgccggaa tggcttgccg ttgattgacc gccctaatgt
     5161 actgcgctat ttctgccgat aagtttccag ttatgggaac taccccgtcc agcacgtcct
     5221 cgttgttcgc tccccaggtt ttttccggag tcagccagtt ctcgagcgat ttccagagca
     5281 tcctgcaacg cgcggtttcg attgccgaac tgcccattca gacgcttcaa aatcagcaat
     5341 ccgatcttct tcaacagaaa cagcttttga tcggcctgaa caccgcggcc gcgagtctcg
     5401 gctccagtct gggcgccctt ggctcgctcg gggcgaatca gggcctctct gccgccagtt
     5461 ccaacagttc aacggtttcc gtcacaaatt ccggcgcaac cagtcccgcc acctacacga
     5521 ttagcaacat tacgtcagtt gccgcggcgg ccgcggagac cagcgtctct gggttcgccg
     5581 attcgacctc ggctcacgtt tcggctaccg gcatcatgaa gttggtggtg ggttcgagca
     5641 cttacgcgat caatctcgct tcggggcaga acaacctgaa cggcctcgcg gatgcaatta
     5701 atagccttgg agcgggcgtc actgcctccg tgctcaccac cgggaccggc ggaac
//
LOCUS       DATNDT010000168         6048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4070678, whole genome shotgun sequence.
ACCESSION   DATNDT010000168 DATNDT010000000
VERSION     DATNDT010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4070678"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VN788_05610"
     CDS             complement(<1..401)
                     /locus_tag="VN788_05610"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXU20042.1"
                     /translation="MPSAIAAEIRSLLAGPLNWDLLLSDAARHTVTPLLAKQLTTVAG
                     DALSAARLKQLKDLARAAVLRSLAFTAELVTILDLFRSEGLQAVPYKGPVLAEQAYGD
                     FALREFDDLDLILRQKDMPKANRLIASLGYRA"
     gene            677..1600
                     /locus_tag="VN788_05615"
     CDS             677..1600
                     /locus_tag="VN788_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXU20043.1"
                     /translation="MDFDHLITFLEIAKQGSFSRAGQKLYRSQPAVSAQIRQLEQEYG
                     QKLFDRVGKTVRLTIAGETLLEYAARLLTLRNESLRAVADQSSTPHGTLALGANEATC
                     LYVLPDIFAEYSRCFPGVQISIYRNFSHKVLEKVEDGTVDVGIVTLPVKSPSLKVHPI
                     FRDRLMLMVSPTNPLAANKNVRTKDIADQPLIFPKTGFTRQVLDKQFRPFRSRLRITM
                     ELASVGMIKRFVAAGLGVSLISEQFAQDEVRAGQVKLIPISDMEMWRELGLVYRRDRT
                     LPRSASAFVALARQGVSGAVAENDGKPAVKR"
     gene            complement(1625..2491)
                     /locus_tag="VN788_05620"
     CDS             complement(1625..2491)
                     /locus_tag="VN788_05620"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HXU20044.1"
                     /translation="MAGTVPFYRVAIIGTGLIGGSFALAIRKQIPSVSLVGYARPDST
                     RQARERGAIEEAVSDLSSALRGADLVYVALPIGASIEMLPAIAAAAEPHALVTDAGST
                     KSAIGHVARNYFRTGARFLGGHPMAGKEVSGIEHADAELFRNAPYALIAAEADPDPRV
                     NCFVELVRAIGARPFWTDDETHDWAVGVVSHLPQLLSVALANVVKDETDETGLPLALA
                     GPGLQDMLRLSGSPYEVWRDIFLTNTENISRALDRLGQAIDHIRTHLATKELESEFHA
                     ANELYGLLHKQS"
     gene            complement(2562..3584)
                     /gene="ilvC"
                     /locus_tag="VN788_05625"
     CDS             complement(2562..3584)
                     /gene="ilvC"
                     /locus_tag="VN788_05625"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HXU20045.1"
                     /translation="MAKMFWEKDANPRALEGKRIAVVGYGSQGRAHALNLRDSKLNVV
                     VGLRRGGPSWAQAEQDGWKLAAPSEASDGADVIVMLAPDMAQPAIYVNEIAPNLKEGA
                     MLLFSHGFNIHYGQITPPKNIDVTMVAPKGPGNLVRRQYEAERGVPCLLAVHQDASGH
                     AFDRTLAYAHAIGGTRPGVIQTTFSEETETDLFGEQAVLCGGARALIQAGWETLVEAG
                     YQPESAYFECLHELKLIVDLIYEGGIAEMHKFVSETAKYGDLTRGNRVIDDNVRKTMK
                     TILDEIQSGQFAKEWVLENQAGRPQYTKLLERDKNHPIEKVGVELRKQMSWLKPESTA
                     AARGKK"
     gene            complement(3606..5255)
                     /gene="ilvD"
                     /locus_tag="VN788_05630"
     CDS             complement(3606..5255)
                     /gene="ilvD"
                     /locus_tag="VN788_05630"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HXU20046.1"
                     /translation="MQSDVIKKGIARSPNRAMLKATGLTDADLARPLVAVANTWSEIT
                     PCNFHLRSLAEKVKEGIRAAGGTPIECNTIVISDGITMGTEGMKGSLVSREVIADSIE
                     LFVRSHAFDAVVAICGCDKTIPGTVMALARLNLPGLSLYGGSIMAGKFQGHDVTIQDV
                     FEAPGAFAAGKMTEKEVWELEDHACPGAGACGGQFTANTMATAITFLGISPMGANEVP
                     AVDPRKAQIAFDCGKQVMQLLKDDIRPKQIITHASLENAIVSVAATGGSTNAVLHLLA
                     IARDAGVALKIDEFDQISAKTPVIADLKPGGRFTAPDMFHAGGMRLLAKRLQEAGLLH
                     DGKTASGHNLFDEIRNAAETPSQEVLRPISNPLKKDGGFAILRGSLAPDGCVVKLAGH
                     ERLLHTGPARVFDSEEAAFEAVQQRKIKPGDVVVIRYEGPKGGPGMREMLAVTGAIVG
                     QGLGDTVALLTDGRFSGATHGLMVGHVAPEAAHGGPIAFVRDGDKITFDVRARRLDLE
                     ANIEERRTQWKPPEPRYTSGVMAKYAKLVSSASEGAVTNAG"
BASE COUNT         1213 a         1741 c         1798 g         1296 t
ORIGIN      
        1 gcacgatagc cgaggctggc gatcagacga ttcgccttgg gcatgtcctt ctggcgcaga
       61 ataaggtcga ggtcgtcaaa ctcgcgcagg gcgaaatctc cgtaagcttg ttcggcgaga
      121 acggggcctt tgtagggaac cgcctgaagg ccttcggagc ggaagagatc taggatcgtg
      181 acaagctccg cagtgaaggc aaggctgcga aggacggcag cgcgcgcgag atctttcaac
      241 tgcttgaggc gtgcggcaga gagtgcgtcg cctgccacgg tggtgagttg cttggcgagg
      301 agtggcgtga cggtatgacg cgcggcatcg ctcagaagca aatcccaatt gagcggacct
      361 gcgagcagcg agcgaatctc agccgcaatt gcggagggca tctttgtccg cgcgcagcag
      421 acgagcagcc gtttttctgg agacgcagtc gcgctcaatg agtgaccgtc cctaacgcgc
      481 aaacaagagc cgcgacaatt ttacgatatc gccagcatgt ccgggaaact cttcgcagtg
      541 ttggcctgcg agcacgggac cgtcctgcca ccatcgggct cgtagcggaa cagggatcga
      601 atttgcttgc caaccgattg atcttaaggc ttgcgagtga gggtctctct agtttgatat
      661 aattcgtcac caatccatgg acttcgacca cctgatcact tttttagaaa tcgccaaaca
      721 gggcagcttt tctcgcgcgg gccagaagct ctaccgttcg caacctgcgg tgagcgcgca
      781 gatcaggcaa ctggagcagg agtacgggca aaagcttttc gaccgagtgg gaaagacggt
      841 gcgcctgacc atcgccggag aaacgctgct cgaatatgcg gcaaggttgc tgacattgcg
      901 caatgaatcc ctacgcgcag tggcggatca atcgagcacg ccgcacggca cgctggccct
      961 tggcgcgaac gaagcgacct gcctctacgt gctgccggat attttcgcgg aatacagccg
     1021 gtgctttccc ggagtgcaga tcagcatcta ccggaatttc agccacaagg tgctcgaaaa
     1081 agtggaagac ggcacagtgg atgtgggaat cgtcacgctt ccggtgaagt cgccgagcct
     1141 gaaggttcat ccaatctttc gcgatcgctt aatgttgatg gtcagcccca cgaacccgct
     1201 ggcggcgaac aagaacgtgc gcactaaaga tatcgccgac cagccgctga tatttcctaa
     1261 gaccggattt acccgccagg tactcgataa gcagtttaga ccattccgtt cacgcttgcg
     1321 gattacgatg gagctcgcca gcgtgggaat gatcaaacgc tttgtggctg cgggactggg
     1381 cgtttcgtta atcagcgaac aattcgcgca ggatgaagtt cgcgccggcc aggtgaaact
     1441 aattcccatt tcggacatgg aaatgtggcg cgagttgggg ctggtctacc ggcgcgaccg
     1501 gacgttgccg cgttcggcgt ctgcgttcgt ggccttggcg cgccagggcg tgagtggtgc
     1561 ggtagccgaa aacgacggta agcccgcagt gaagcgctga gatttgtgat cgagggccgc
     1621 aaagctacga ctgcttgtgc aggaggccat acagttcgtt tgcggcgtgg aattcggatt
     1681 caagctcttt ggtagccaag tgagtcctga tgtggtcaat agcctgtcct aggcggtcga
     1741 gcgcgcgcga aatattttcg gtgttggtga gaaagatgtc gcgccaaact tcgtagggac
     1801 ttcccgagag ccgcagcata tcctgcaagc ccggtcccgc aagcgccaga ggcaggcccg
     1861 tctcgtccgt ttcatcttta accacgttcg ccagcgcgac ggacaaaagt tgcggcagat
     1921 gcgagacgac gccgaccgcc caatcatgag tttcatcgtc agtccagaag ggccgcgccc
     1981 cgatggcgcg gaccagctcc acgaagcaat ttacgcgagg atcgggatcg gcctcggccg
     2041 cgatcaatgc gtacggtgca ttacgaaata gttccgcgtc ggcatgctcg ataccggaaa
     2101 cttcctttcc cgccatgggg tggccgccta gaaagcgcgc gccggtgcgg aagtagttcc
     2161 gagccacgtg gccaatcgcg gatttcgtgc tgccggcatc ggtaaccagg gcgtgcggct
     2221 cggcggcagc ggctatagcg ggcagcattt cgatcgacgc gccgatcggc agcgcgacgt
     2281 aaacgagatc ggcgccgcgc agtgcactag aaagatcgga gacggcttcc tcaattgcgc
     2341 cgcgttcgcg agcttgccgc gtggagtcag ggcgggcgta acccaccaga gagacgctgg
     2401 gaatttgctt gcggatcgcc agcgcaaacg atccgccgat caggcctgtg cctatgatag
     2461 cgacacggta aaagggaact gtgccggcca tgagtcaggc ccggcgcatc atgccgttgc
     2521 ttgcggtgcg cacgtggatt ggagagacct cgcggcggcg actatttctt tccacgcgcc
     2581 gctgccgttg attcgggttt cagccacgac atctgctttc gtagttccac gccaaccttc
     2641 tcgataggat gattcttgtc gcgttcgaga agcttcgtat attgcggccg gccagcttga
     2701 ttttcgagta cccactcttt cgcaaattgt ccggattgaa tttcgtcgag gatcgttttc
     2761 atcgtcttgc gaacgttgtc gtcgatcacg cggttaccgc gggtcaggtc gccgtacttg
     2821 gcggtttcgc taacgaactt gtgcatttcc gcgatgccgc cttcgtagat cagatcgacg
     2881 atcagcttca attcgtgcag gcattcgaag taggccgatt cgggctgata gcccgcttcc
     2941 accagggtct cccatcctgc ctgaatcagc gcgcgcgcgc cgccgcatag gacggcttgc
     3001 tcgccgaaga gatccgtctc ggtttcttcg ctgaaagtcg tttgaatcac gccgggccgc
     3061 gttcctccga tggcgtgcgc gtaggccagc gtgcgatcga atgcgtgacc cgaggcatcc
     3121 tggtgcacgg cgagcaagca gggaacgccg cgctcggctt catattgccg gcgaaccaga
     3181 ttaccgggcc ccttgggcgc gaccatagtg acgtcgatat tcttgggtgg cgtaatttgg
     3241 ccgtagtgaa tattgaagcc atgcgagaaa agcagcatgg ctccctcttt cagattggga
     3301 gcgatttcgt tcacgtaaat cgcgggctga gccatgtcag gcgcgagcat cacgatcacg
     3361 tccgcgccgt cagacgcctc ggatggcgcg gcgagtttcc agccgtcttg ttctgcttgg
     3421 gcccaggacg gtccgccacg gcgcagcccc actacgacgt tgagcttgct atcgcgcagg
     3481 ttcagcgcat gggcgcgtcc ttggctacca tatccgacta ctgcgatgcg ctttccttcc
     3541 agggctcgcg gatttgcgtc tttttcccag aacatcttgg ccatgtgttt ttctccttgg
     3601 aatctctatc cggcgttggt aacggcgcct tcggatgccg atgaaacgag ctttgcgtat
     3661 ttggccatca ccccactcgt gtagcgtggc tcgggaggct tccactgcgt gcggcgctct
     3721 tcgatattgg cttccaaatc gaggcgtctg gcgcgcacgt cgaaagtgat cttgtctcca
     3781 tcgcgcacga acgcgatggg gccgccatgc gccgcttccg gcgccacgtg gccgaccatc
     3841 agtccatgag tggcgccact gaagcgtcca tcggtaagca gtgccacggt gtcgccgagg
     3901 ccttggccta cgattgctcc tgtaacggcc agcatttcgc gcattccggg gccgcccttc
     3961 ggtccctcgt agcgaattac gaccacgtcg ccgggcttaa ttttccgctg ctgcacggct
     4021 tcaaacgccg cttcttcgga atcaaacacc cgcgccggcc cagtgtgaag cagccgttcg
     4081 tgccccgcaa gcttgacaac acatccatcc ggggcgaggg aaccgcgaag gatagcaaat
     4141 ccgccgtcct tttttagtgg atttgaaatc gggcgcaaga cctcctgcga aggcgtctcg
     4201 gcggcattcc gaatctcgtc gaaaagattg tgtcccgacg ccgttttccc atcatgcagc
     4261 aagcccgcct cttggaggcg tttcgcgaga agccgcatgc cgcccgcatg aaacatgtca
     4321 ggcgctgtga accgaccgcc gggcttgagg tccgcgatca cgggagtctt cgcggaaatt
     4381 tgatcgaatt cgtcgatttt caaggccacg cctgcatcgc gagcaatggc cagcagatgt
     4441 agcacggcgt tggtcgagcc tccggtggca gctacggaaa cgatcgcatt ttcgagcgac
     4501 gcgtgagtga tgatttgttt tgggcggatg tcgtccttca gaagctgcat cacctgtttc
     4561 ccgcagtcaa acgcaatttg cgctttgcgc gggtccactg ccggcacttc gttcgcgccc
     4621 atcggggaaa tgccgagaaa agtgattgcc gtggccatgg tgttggcggt gaattggccg
     4681 ccacaagccc ccgctcctgg gcacgcgtga tcttccagtt cccatacttc cttctcggtc
     4741 attttacctg ctgcgaaagc tccgggagcc tcgaacacgt cttgaatggt cacgtcgtgc
     4801 ccctgaaact tccccgccat gatggagccg ccatagagcg aaaggccagg cagattgagc
     4861 cgcgcaaggg ccatcaccgt gcccggaatg gttttgtcgc agccgcaaat cgcgactacc
     4921 gcatcgaacg cgtgcgaacg cacgaaaagc tcgatggaat ccgcaatcac ttcgcgcgat
     4981 accagcgaac ctttcatgcc ttccgtcccc atggtgattc cgtcggaaat cacgatggta
     5041 ttgcattcga tcggtgtgcc gcccgcggct cgaatgcctt ccttcacctt ctccgccagc
     5101 gaacgaagat gaaaattaca aggcgtgatt tccgaccagg tattcgctac agccacaagc
     5161 ggccgcgcca gatcggcgtc ggtgagcccc gtggccttca gcatcgcgcg gtttggagaa
     5221 cgcgcaattc cttttttgat gacgtcactt tgcatgttac ttggcctata tcctgtctag
     5281 ctcttccgcg aattcgacgg ggtgagagcc gttcgtggtg ccagcgtctc ggtggatccc
     5341 ttactgtctt ccgctcgcgg gaagtgctgc ttcagcttcg gtttttccgc aacaaaaaag
     5401 ccccgctacc tagcttccgg ggggcggggc gcacatgcct aagcttggct taggatggcg
     5461 gtccccatct ggctactaca tatacgggaa tcggaatcgg cagcgtcata gagtaacgtc
     5521 tcacttgcca atcgacaatt agttttctgg agcgaaaggc cggtcggctc agcaccgcca
     5581 gttacgcctg caagcccgat taggctcgtt cctggctgat aagtcaaatt tggaatcatt
     5641 gcaaccaata taaatcgcac ttatgcctga ctcgtcgaag aagcgcttga cggtccaggt
     5701 aagcgttcgg tatagttgcg attcaattca ggagcgttag accgcgccat gagcatcgct
     5761 tatcgaaact tcgcctttac ctaccgcttc ctctttttgg ggaagcgggc cggcgaaacg
     5821 cgcgcggatg gaatgaacag ctaaaagccg cacaaagttt caaggaccgg gccgcgactc
     5881 cgaacgggga cgcggccttt tttgttggcg gaaggatttg tggggccgac atacacaggg
     5941 acgcgacgtc atttccagtg tgccgcgcga taacataagc gggctcaact ttgcatttta
     6001 ggagaagcac gatgacggaa cgaccgcgaa tcatagactg gcgaagca
//
LOCUS       DATNDT010000169         4271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1168727, whole genome shotgun sequence.
ACCESSION   DATNDT010000169 DATNDT010000000
VERSION     DATNDT010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1168727"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="VN788_05635"
     CDS             complement(<1..275)
                     /locus_tag="VN788_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU20047.1"
                     /translation="MSGDEKVNILMVDDQPAKLLSYEAILHELGENLIKASSGREALE
                     HLLKNDIGIVLMDVSMPELDGFELAEMIRQHPRFQKTAIIFISGVHLT"
     gene            complement(338..>4271)
                     /locus_tag="VN788_05640"
     CDS             complement(338..>4271)
                     /locus_tag="VN788_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU20048.1"
                     /translation="RKITVDVKGEILELKNTINTMVDQLNAFAGEVTRVAREVGTDGK
                     LGGQAEVPGVAGTWKDLTDNVNFMASNLTGQVRNIAEVATAIATGDLSKKITVDVRGE
                     ILQLKETLNTMVEQLRSFAAEVTRVAREVGSEGRLGGQANVPGVAGTWKDLTDSVNAM
                     AGNLTAQVRNIAEVTTAVARGDLSRKITVDVKGEILELKNTINTMVDQLNAFAAEVTR
                     VAREVGTGGKLGGQAQVPGVAGTWRDLTDNVNVMAANLTEQVRGIVKVVTAVANGELT
                     QKLTVNAKGEVAALAETINNMTNTLATFADQVTTVAREVGVEGRLGGQANVPGAAGTW
                     KDLTGNVNLLADNLTNQVRAIAEVATAVTKGDLTRSIQVEASGEVAELKDNINTMIDN
                     LRLTTDRNKEQDWLKTNLARFTGMLQGQRDLTTVGRLLLSELAPLVGAHQGVIYRMES
                     EAAGMELLSAYADDELDGHRQHLAMGEGLVGQCAVEKRSMLISEMPMHSVPIRSGLFQ
                     ALPQNVIVLPVLFEDRVKAVIELASLSRFTASHLAFLEQLTASIGIVLNSIEATMQTE
                     GLLKQSQQLATELQTQQKELQQTNEQLAQKAQQLAEQNVEVERKNQEIEQARRALEEK
                     AKELALTSKYKSEFLANMSHELRTPLNSILVLGQQLTDNPDRNLSPKQVEFARTIHAA
                     GTDLLNLISDILDLSKIESGTVSVEAEEVFFSSLLDMVARPFRHEAENRRFTFEVRTE
                     PSLSRGLVTDSKRLQQVLKNLLSNAFKFTEQGSVCLSVSAVASGWSQDHPVLSTAPSV
                     IAFEVSDTGIGIPPEKQRIIFEAFQQADAGTSRKYGGTGLGLAISRELASLLGGEIQL
                     HSSPGQGSTFTLYLPQIYVGPSTGIVSVDRKTAGASNLPAQLASTMSERSMPQVRDDR
                     ESLDPGDAVLLIVEDDPHYATILRDLSHEKGFKVLIAPRGADALALAREFQPAAISLD
                     VFLPDMLGWTVLNHLKQDPTTRHIPVQMLTLDEDRNHGLSRGAFAFVTKPTTPEGLDS
                     AFTRIKEYAAPRRKRLLVVEDDPAEQFSIRELLGYDDIDVSIAATGKEALASIGTEPY
                     DCMVLDLRLPDMSGFDVLERLRDMPSSGDLPVVVFTGKELTSEEDLQLHALARSVVVK
                     GVESPERLLDETALFLHRVVADLPANKQEMLRRLHSSDEALAGKKVLLVDDDVRNIFA
                     LSSMLERRGMMVLTAGTGREAISILEATEDIAIVLMDIMMPEMDGYETMQVIRQNPSS
                     RRLPIIALTAKAMKGDREKCLEAGASEYLAKPVNTDQLLSVLRMWLHR"
BASE COUNT          823 a         1333 c         1121 g          994 t
ORIGIN      
        1 gtgagatgga cgccggaaat gaaaatgatc gccgtctttt ggaatcgcgg atgctgtcgg
       61 atcatttccg ccaactcgaa accatcgagt tccggcatgc tcacgtccat tagcacgatt
      121 ccaatatcgt tcttcagcaa atgctccagc gcctctcttc ccgaactagc cttgatcagg
      181 ttctcaccga gttcgtgaag aatagcttcg taactgagca gcttcgccgg ctggtcatcg
      241 accatcagga tgttgacctt ttcgtccccg ctcatatccc tccttccggc agtgtgcaaa
      301 atgctactaa aaccgttgct ctaacgaaca ccaccgccta tcgatgcagc cacatgcgaa
      361 gcactgaaag cagctgatcc gtattcaccg gcttcgcaag gtattcggag gcgccggctt
      421 ccaggcactt ttcgcggtcg cccttcatgg ccttggcggt caacgcgatg atcggcagcc
      481 gccgcgatga agggttttgc cggatcacct gcatggtctc gtatccgtcc atttctggca
      541 tcatgatatc catcaagacg atcgcgatat cctccgttgc ctccaaaatg gaaatcgcct
      601 cgcgtcctgt gcccgccgtg agcaccatca tgccccgcct ttcgagcatg ctgctcaacg
      661 caaagatgtt tcgcacatcg tcatccacaa gcaatacttt cttgccggcc agcgcctcat
      721 cggagctatg cagccggcgc agcatttcct gcttgttcgc gggaaggtcg gcgactaccc
      781 gatgcaagaa gagggctgtc tcgtccagga gcctctcggg tgattcgaca cccttcacca
      841 ccacacttcg ggccagcgca tgcaattgca agtcttcctc cgacgtcaac tctttgcccg
      901 tgaacaccac caccggcagg tcgccggacg acggcatatc tctaagccgt tccaacacgt
      961 cgaaaccgga catatccggc agccgcaaat caagcaccat gcagtcatag ggctcggtcc
     1021 cgatggaagc gagagcctct ttgccggtgg cggcaatcga aacgtcgata tcgtcgtatc
     1081 ccaacaattc gcgaatgctg aactgttccg ccgggtcgtc ttcaaccacc aacagccgct
     1141 tgcgccgtgg cgcggcatac tccttaatac gagtgaaagc cgaatcgagg ccctcgggtg
     1201 tagtaggttt cgtcacgaaa gcgaaggccc cgcgcgatag gccgtggttc cgatcctcat
     1261 cgagggtcag catctgcacc ggaatgtggc gcgtggtggg atcctgcttt agatgattca
     1321 gcacggtcca acccagcatg tctgggagga aaacatccag cgagatagcc gcgggttgaa
     1381 attcgcgagc caaagcgagc gcatccgccc cccgaggcgc aatcagaact ttgaatccct
     1441 tctcgtgaga aaggtcccgg agaatggtcg cgtaatgagg atcatcctca acgatgagca
     1501 aaacggcgtc gcctggatcg aggctttcgc gatcgtcgcg aacctgaggc atagatcgct
     1561 ctgacattgt acttgccaat tgagccggca ggttcgacgc ccccgccgtt ttccggtcca
     1621 ctgaaacgat ccccgtggaa ggacccacat aaatctgagg caagtagagt gtgaacgtgc
     1681 tgccctgccc cggcgaactg tgcagttgta tttcgccgcc cagcagactc gcgagctcgc
     1741 ggctgattgc gagacccaga cccgtgccgc cgtatttgcg actggttccc gcgtccgctt
     1801 gttggaaggc ctcgaagatg attcgctgct tctcgggggg aatgccaatc ccggtatcgg
     1861 aaacctcgaa tgcaatgacg ctaggcgctg tcgataaaac cggatgatcc tggctccaac
     1921 cgcttgcgac cgccgatacg gacaggcata cgctgccttg ctcggtgaac ttgaacgcgt
     1981 ttgaaagcaa atttttcagc acttgctgta aacgcttgga gtccgtcacg agaccgcgtg
     2041 acagggaggg ctcggtccgc acctcgaaag taaaccggcg gttctcggcc tcgtggcgga
     2101 agggccgagc caccatatcg agcaggctgc tgaagaatac ttcctcggct tccacggaaa
     2161 cggtcccgga ctcgatcttg gatagatcta aaatatcgct gatcaaattc aagaggtccg
     2221 ttccggcggc gtgaatcgtc cgcgcgaact caacctgctt cggtgatagg ttgcgatcgg
     2281 gattgtccgt aagctgctgg cctaaaacca aaatgctgtt tagcggagtg cgcagttcgt
     2341 gcgacatgtt cgccaagaat tcggacttgt actttgaagt gagggccaat tccttggctt
     2401 tttcttccag cgctcgacgg gcctgctcga tttcctgatt tttgcgctcc acttccacgt
     2461 tctgttccgc caattgctgg gctttttgcg ctaactgctc gttggtctgt tgaagctcct
     2521 tctgttgagt ctgaagctcg gtggccaatt gctgggactg cttcaaaagc ccttcggtct
     2581 gcatcgtcgc ttcaatactg ttgaggacga ttcctatgct ggcggtcaat tgctcgagaa
     2641 aagcgagatg cgacgccgtg aatctgctca gcgaggcgag ctcaatcacc gcctttacgc
     2701 ggtcctcaaa caggaccgga agcacgatca cattttgcgg cagcgcttga aagaggcccg
     2761 atcgaatagg aacgctgtgc atcggcatct cgctgatgag catgcttctc ttttccaccg
     2821 cgcactggcc caccagaccc tcgcccattg ccaggtgctg tcggtgtcca tccagttcat
     2881 cgtcggcgta cgcggagagc agctccatcc cggccgcttc actttccatt ctatagatca
     2941 cgccctggtg agcacccacc agcggcgcta actccgacag cagcaggcgg cccacggtgg
     3001 tcagatcgcg ctgcccttgc aacatgcccg taaatcgagc caggttcgtc ttcagccagt
     3061 cctgctcctt gtttcgatcg gtggtgaggc gcaggttgtc gatcatcgtg ttgatgttgt
     3121 cttttagctc ggcaacctcg ccgctcgcct ctacttgaat cgaccgcgtc aagtcgccct
     3181 tggtaacggc cgttgcgact tccgcaatcg cgcgtacttg gttggttaga ttgtctgcga
     3241 gcaggttgac gttgccggta agatctttcc acgtacctgc ggcgccaggg acgttagcct
     3301 ggccgcccaa gcggccttcc actcccacct cgcgcgccac agtggtcacc tgatcggcga
     3361 aggtggccag cgtgttggtc atattgttaa tggtctctgc gagagccgct acttctccct
     3421 tggcgttcac cgtgagtttc tgcgtcaatt ccccgtttgc caccgcggtc actactttaa
     3481 cgattcctcg cacttgctcg gtcaagtttg cagccattac gttgacgttg tcggtcaggt
     3541 cgcgccacgt gccggccact ccgggcactt gcgcctgccc gccgagtttt ccgccggtac
     3601 ctacttcgcg cgccacgcgc gtcacttcgg ctgcgaaagc gttgagctgg tccaccatcg
     3661 tgttgatcgt gttcttcagt tccagaattt cgcccttcac gtccactgta attttgcgcg
     3721 acaaatcgcc gcgcgccacc gccgttgtca cctcggcaat gtttcgaacc tgagccgtca
     3781 agttgccggc cattgcgttc actgagtctg tcaaatcctt ccacgttccc gccacacccg
     3841 gcacgttggc ctggccgccc agcctgcctt cgctacccac ctcgcgcgcc acgcgcgtta
     3901 cttcggcggc gaacgaccgc aactgttcca ccatcgtgtt cagcgtttct tttagttgca
     3961 aaatttcgcc gcgcacatcc acggtaatct tctttgataa atcgccggtg gcgatggccg
     4021 ttgccacttc ggcaatgttc cgaacctggc cggtcaaatt gctggccatg aagttcacgt
     4081 tgtcggtcaa atccttccac gttcccgcca cgcctggcac ttccgcctga ccgccaagtt
     4141 ttccgtccgt gcccacttcg cgcgccacgc gcgtcacttc gcctgcgaag gcattcaact
     4201 ggtccaccat cgtattgatc gtgttcttca gctccagaat ttcgcccttc acgtcgaccg
     4261 tgatcttgcg c
//
LOCUS       DATNDT010000170         3271 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3455369, whole genome shotgun sequence.
ACCESSION   DATNDT010000170 DATNDT010000000
VERSION     DATNDT010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3455369"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..232
                     /locus_tag="VN788_05645"
     CDS             <1..232
                     /locus_tag="VN788_05645"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HXU20049.1"
                     /translation="DYLKENTAFQKECPKIRMEFPPFSTWLVFTDGVAHAAMSGQYAI
                     EQTFLISPQVLVAPEQAPCRILETIAGRPLVY"
     gene            311..1780
                     /locus_tag="VN788_05650"
     CDS             311..1780
                     /locus_tag="VN788_05650"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HXU20050.1"
                     /translation="MKRSGVGIIVVMKAISSRNLRTFLLRNREAIEVVVLAAVYFVAG
                     KFGLTMAFVHPSSTAVWPPAGIALAALLIFGYRVWPGIFIGAFLVNLTTAGSVVTSLG
                     IAAGNTLEPVLGAYLVSKFANGRKCFESVHDTVKFAILAGVIGTTVSATMGVTSLSVG
                     GFAKWADFKVIWATWWLGDTVGAVVVAPLLILWSANPKVRWSWNRLSEFALLLISAMV
                     AGQFVFGPFVFPGLKNVPLEYWCIPFLIWAAVRFGPREAATIVAVLSGIAIRGTFFGF
                     GPFAIGTRNESLLLLQAFMGIIAVMTLTLAVMSEERRRAEERIRNLAVTDALTGLANY
                     GKLIDVLDSEMKRSNRTGRPFAVLLMDLDGLKKINDQHGHLTGSGALRRIADILRVYC
                     RDTDTAARYGGDEFALVISEGGSKEARQVASRIRERVSQDAGIPPLSISVGAAAYPQD
                     GCTREELLEAADRELYAMKRLPLGRMSSPESTSLLRSGR"
     gene            complement(1804..>3271)
                     /locus_tag="VN788_05655"
     CDS             complement(1804..>3271)
                     /locus_tag="VN788_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU20051.1"
                     /translation="DDEIEIREGLEALLTSESFDVTLAETGAAGLQKLDDRPFDLMLL
                     DVSLPDRNGLDMLREIRLRDPHLSIILITAYGSIEMARAAFKGGAQDYITKPWSNDEL
                     IAQISLAIEGRRLREENLHLKRALKQRYNFPNIVGKSEKMLAVLDLVTQVAPSRSTVL
                     ISGESGTGKEVIAKAIHAASTRAERAFIPINTGSIPVDLLESQLFGHVKGAFTSAIAA
                     KKGLFEVADQGTIFFDEISTVGPETQAKLLRVIQEREFMRLGGTETIKVDVRILAASN
                     EDLRKLVREGRFREDLFHRLNVISIQLPPLRDRREDIPLLVDRFLDQFCRENGKALRT
                     FSNRAMKLLMDYEWPGNVRELENAVERAVVLSTAESMDADLLPENIHSREIVKGVRFQ
                     LAEFMRSPQEGADLRNAVPPPSLFEILEEVERRVIVDMLERTNWNQTEAAERFQVPLS
                     TLNQKIKRLGIEIRRRPGVPRVPDSYGEDGPALSPGNC"
BASE COUNT          649 a          938 c          926 g          758 t
ORIGIN      
        1 cgattacctg aaggagaaca ccgcgtttca gaaggagtgt cccaagattc gaatggagtt
       61 tccgccgttt tccacgtggc ttgtatttac cgacggcgtc gcgcacgcgg cgatgtcggg
      121 acagtatgca attgagcaaa ctttcctcat ttcacctcaa gtgctggttg ctcccgagca
      181 ggcgccctgc cgaatcttag agaccatagc cggccgccct ttggtgtact aagtactttc
      241 actctcatcg cactgagcct acagcatttc gcgtataacg ctttggcgct tcgcttttca
      301 ttcagacgga atgaaacgct ccggcgtcgg catcatagtt gtgatgaaag caatttcctc
      361 aagaaacctt cgaaccttcc tccttcgaaa cagagaagcg atcgaggttg tggtattggc
      421 ggcagtatat tttgtcgctg gaaaattcgg gctgacgatg gccttcgttc atcccagctc
      481 gactgccgta tggccgccag cgggcatcgc gcttgccgct ctgctcatct ttggctatcg
      541 cgtctggcct ggtattttca tcggagcgtt tcttgtgaac ctgaccaccg caggttccgt
      601 ggtcacctcg ctcggaatcg cggcgggcaa tactttggaa cctgttttgg gagcctatct
      661 cgtttccaag ttcgcgaacg ggcgaaaatg cttcgagtct gtgcatgaca cggtgaagtt
      721 cgcgattctc gcgggcgtga tcggcaccac ggtgagcgcc acgatgggcg tcacatcgct
      781 ttcggtggga ggttttgcga agtgggcgga tttcaaggta atttgggcca cctggtggct
      841 tggagacacc gtaggcgccg tagttgtggc gccgctacta atcctatgga gcgccaaccc
      901 taaagtccgc tggagctgga accggctgag cgaattcgcg cttttgctca tatcggcgat
      961 ggtggcggga caattcgttt tcggtccatt tgttttccca ggcttgaaga acgtgccact
     1021 cgagtattgg tgcatcccat ttctgatatg ggcggccgtt cgcttcgggc cgcgagaagc
     1081 ggcaaccatc gtggccgtgc tttcggggat cgctatacga ggcacatttt tcgggttcgg
     1141 cccgtttgca atcggcacgc ggaatgaatc gctgctgttg ctgcaagcct tcatgggcat
     1201 catcgccgtg atgacgctga cgctcgcggt gatgtccgag gagcgccgcc gggcggaaga
     1261 gcgtatccgc aacctcgccg tcaccgatgc ccttactggc ttggcgaact atggcaagct
     1321 catcgatgtg ctcgattcgg aaatgaagcg atcgaatcgc accggaagac ccttcgcggt
     1381 tttgctgatg gatttggatg gcttgaaaaa gatcaacgac cagcatggcc atctgaccgg
     1441 cagtggcgcc ctgcgccgca tcgccgatat tctgcgggtc tactgtcgcg ataccgatac
     1501 cgccgctcga tatggaggcg atgagtttgc gctggtgatt tcggaaggtg gctctaagga
     1561 agctcggcag gtcgcgtcac gcatacgcga acgcgtttcc caggacgccg ggattcctcc
     1621 gctttcgatc agtgtgggcg cggcggccta tcctcaggat ggatgtacgc gggaagaatt
     1681 gctcgaagcc gcggatcgcg aactttacgc gatgaagcgg ctgcctctag gacgaatgtc
     1741 ctcgcctgaa tcgacgtcac ttctgcgaag tggcagataa cagcgtccaa atcgtccggg
     1801 gcattaacaa tttccggggg atagcgccgg tccatcttca ccatagctgt cggggacgcg
     1861 ggggacaccc gggcggcggc gaatctcgat gcccagacgc ttgattttct ggttaagcgt
     1921 cgagagaggc acttgaaagc gttccgcggc ttcggtttga ttccaatttg tgcgttcaag
     1981 catgtccacg atcacgcggc gctccacttc ttccagtatt tcaaataggg aaggaggtgg
     2041 tacagcgttt cgaagatcag ctccctcttg cggcgaccgc atgaattcag cgagttggaa
     2101 tcgcacgccc tttacaatct cccgcgagtg gatattttcc ggtagcaaat ccgcatccat
     2161 cgactcagca gtcgaaagca ccaccgcgcg ctccacggcg ttctcaagct cgcgcacatt
     2221 cccaggccat tcataatcca tcagcagctt cattgccctg ttgctgaatg ttcgaagcgc
     2281 ctttccattc tcgcgacaaa actggtccaa aaaccgatcc acgagaagag gaatatcttc
     2341 gcggcggtcg cgaagcggcg gcagttgtat cgagatcacg ttcaggcgat gaaataagtc
     2401 ctcgcggaac cggccttcgc ggactaactt ccggaggtct tcattcgacg cagccaggat
     2461 gcgtacatct accttgatcg tctccgtgcc tcccaggcgc atgaactccc gctcctgaat
     2521 cacccgtagc agtttggctt gcgtttccgg acctactgtg gatatctcgt cgaaaaaaat
     2581 cgtcccctgg tcggccactt caaacagccc cttcttggca gcgatcgcgc tggtaaacgc
     2641 tccttttacg tggccgaaaa gctgcgattc cagcaggtca actggaatgg agccggtatt
     2701 gatgggaatg aaagcccttt ccgcgcgagt cgacgcggca tggatcgcct tcgcaatcac
     2761 ttccttgccc gtaccgcttt cgcccgagat cagcaccgtc gagcgcgatg gcgccacttg
     2821 cgtcaccaag tcgagcacgg ccagcatctt ctcgctcttt cccacgatat tcggaaagtt
     2881 gtagcgctgc ttcaatgcgc gcttcaggtg cagattctct tcgcgcaggc ggcgcccctc
     2941 gatggccaga gaaatctgcg cgatcaattc atcgttggac cagggcttcg tgatgtaatc
     3001 ctgcgcgcct cctttgaaag cggcgcgcgc catctcaatc gagccgtacg ccgtgatcaa
     3061 aatgatcgaa agatgcgggt cgcgcaggcg gatttcacgg agcatgtcga ggccgttgcg
     3121 gtcaggcagg ctcacatcca gcagcatcag atcgaacggc cggtcgtcaa gcttttgcaa
     3181 acccgccgcg ccggtctcag ccagcgtcac gtcgaagctc tcggaagtca gcaacgcctc
     3241 gaggccttcg cggatttcaa tctcgtcatc g
//
LOCUS       DATNDT010000171         5331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3459473, whole genome shotgun sequence.
ACCESSION   DATNDT010000171 DATNDT010000000
VERSION     DATNDT010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5331
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3459473"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..173)
                     /locus_tag="VN788_05660"
     CDS             complement(<1..173)
                     /locus_tag="VN788_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013045231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HXU20052.1"
                     /translation="MTDSKKFYLTTPIYYANSRPHVGSAYTTIVCDVIARYKRMCGYE
                     VAYLTGTDEHGVN"
     gene            327..578
                     /locus_tag="VN788_05665"
     CDS             327..578
                     /locus_tag="VN788_05665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20053.1"
                     /translation="MLDQGFWAEIKTDGEHLRLFSEHNAQGVQASVFDVKNKRWIAPS
                     EPVGDIDEGKEKATEYAKAHLERLGIELPPLNWKMSRAV"
     gene            complement(609..1085)
                     /locus_tag="VN788_05670"
     CDS             complement(609..1085)
                     /locus_tag="VN788_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20054.1"
                     /translation="MTLAVSIPAAVPPQGDPVDFNACTVFSAADAAQVLGVAVRPITS
                     VGGCSYEAAKETSSGWRRNTAVNVYKYKSAADETSAWGDQKILHHFEPGRKNLTVVSG
                     IGSEAYLQVVPDRNAFEATIWVHKNLSHFRLVALSEQSPSPAVMKAIAQKIAAKLP"
     gene            1319..1927
                     /locus_tag="VN788_05675"
     CDS             1319..1927
                     /locus_tag="VN788_05675"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXU20055.1"
                     /translation="MATVRGRNADLHGNVPDASPIALILVDVINALDFPNNGYLLKRV
                     PRLAKNIATLKTRCRERRIPAIYVNDNHDKWRSDRSILIAHARSANPVSRLLTEALLP
                     QDDDYFILKPKHSAFYSTPLDAILSKLGTRTVILAGLTTSACILLTAGEVYVRDFKLF
                     VPSDCVAGLNRPDHLKALSLMRTSFQADTRPYRKLNLRSLAS"
     gene            1998..2729
                     /locus_tag="VN788_05680"
     CDS             1998..2729
                     /locus_tag="VN788_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU20056.1"
                     /translation="MPSPSAANRIRRIVIVAGGTGGLGRAVALAFLSEGDTVAVPYRN
                     ESEFAALKSQAGESAAALHGQQVDVTDESSVKKFIDGVVAQHGHIHALVNTVGGYIGG
                     PKLWELADADLDRMLTLNLRSGFVLARAAVRVMLRQGNGSIVNVAARAALEPAAGASA
                     YAASKAAAVAMFDSLAAELRGTGVRANSILPSIIDTEANRKAMPDANFSKWPKPEDIA
                     RVILFLCSDEAKLIHGAAIPVYGNA"
     gene            3007..3522
                     /locus_tag="VN788_05685"
     CDS             3007..3522
                     /locus_tag="VN788_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HXU20057.1"
                     /translation="MTIAKVTLVAFLSSLMGLLAVPASAQGRTDAAAIRNIVEGETVA
                     WNKADPVAYASHFAADGTFTNILGAFYKGHDAFLAEHTQIFKTIFLGSTLQQDIVSLQ
                     FPTPDVAVVEVLTAVTGAKIPGARSDSFDAKGRLRTRLLQVIARRNGKWEIVAYHNVE
                     VKDALHAPEPQ"
     gene            4113..4628
                     /locus_tag="VN788_05690"
     CDS             4113..4628
                     /locus_tag="VN788_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02391 family protein"
                     /protein_id="HXU20058.1"
                     /translation="MSDGEIQRDHVARTEVRKALLQGFVEQLEDLAPPSARTAPDRYT
                     FHSEIERVGGDLYRNGHYKSAALESYIRVIRQVRDISRIDDDGDSLMNKAFACDKQTP
                     VIQFNTLQTESERDEQRGIYFLFKGIVGLRNSKAHDNRLFDDPLRAHEYLALASLLMR
                     LLEIARVNPLP"
     gene            complement(4657..>5331)
                     /locus_tag="VN788_05695"
     CDS             complement(4657..>5331)
                     /locus_tag="VN788_05695"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXU20059.1"
                     /translation="RPIYTFGYFTGMRLGELKNLTWSRVDHQTETIRLESEDTKNAEP
                     RAIPYGKVPELAQVMAELWKTTKCASGLVFTRKGVAPLGSFRKAWIRACIKASITTKS
                     GVSRMLWECPTCHDRREVEKQPWPPELPKDEAPRCAKCEKSFRWTYCGLIFHDLRRAG
                     VRNLVRAGVPQSVAMRISGHKTTAVFQRYNITDEKDIAEAMERVAEFHVVEAKRTEAS
                     PNVRPN"
BASE COUNT         1216 a         1552 c         1428 g         1135 t
ORIGIN      
        1 atattgacgc catgctcatc cgtgccggtg agataggcga cttcatagcc gcacatgcgc
       61 ttataacgcg cgatcacgtc gcaaacgatc gtcgtgtaag ccgagccgac gtgcgggcgc
      121 gaattggcgt agtaaatcgg agtagtaagg taaaactttt tggaatcagt catttgtagt
      181 cggtttctag gttatcgcaa ccagcgccgt tcgcgaaagc ggagagaacg gcttaccggc
      241 gccactcagt ctcgcggatt cgtcagcccg caatcccatc gttcgtgtaa gaatgaatac
      301 cggctgaaga ttgtaagagg atcccgatgc tcgaccaagg tttttgggct gaaataaaga
      361 ctgacggcga acacctgaga ctattctcgg agcacaacgc gcaaggagtt caggcgtcgg
      421 tgttcgacgt gaagaacaag agatggatcg ctccttccga gccggtggga gacatcgacg
      481 agggcaaaga gaaggccacc gaatacgcga aggctcacct ggagcgcctc ggcatcgaac
      541 tcccgcccct caattggaaa atgtcccgcg ctgtgtaaca ccgcacagaa tccgctgcag
      601 aggctgcctt acggaagttt ggcggcgatt ttttgtgcga tagccttcat tacggccggc
      661 gaagggctct gctcgctcaa cgcaacgaga cgaaagtgcg aaagattctt gtgcacccag
      721 atagtcgctt cgaacgcgtt tcggtcgggc acgacttgta agtacgcttc ggaaccgatg
      781 ccactcacca cggtgagatt tttcctccct ggctcgaagt gatgcagaat tttctgatcg
      841 ccccatgcgg acgtctcatc ggccgcggat ttgtacttgt atacatttac ggccgtgttc
      901 cgccgccagc cactggacgt ttccttcgcc gcttcgtacg agcagccgcc aacactggta
      961 atcggacgca ccgccacgcc gagcacctgt gccgcgtcag cagcactaaa gacagtgcaa
     1021 gcgttgaagt ccactggatc gccttgcgga ggcaccgccg ccggaatcga aactgccaat
     1081 gtcataagta ctagcaggca aacacacgag ctaaccgcaa cggtccggcc ccgaacaaag
     1141 gcgccgctct cactcccatc ccacgtctcg ccttggaaag acataagtca cctttcgagt
     1201 tggttggaaa tcatgcagcg aggccggcgc gccgcatgag tccagatcat actatcgcaa
     1261 gcgtaatcgg caaatgccgt ggccgagcgc atgaatgacc atgccataat cgttctgtat
     1321 ggcaactgtt cgcggcagga acgccgatct tcacggcaat gtccccgacg cttccccgat
     1381 cgcgcttatc ctcgttgatg taatcaatgc tctcgatttt cccaacaacg gctacctgct
     1441 gaagcgggta ccgagacttg cgaaaaacat cgcgacccta aaaacgcgct gccgcgaacg
     1501 ccgcattccg gcgatctatg ttaacgacaa ccacgataaa tggcgctcag accgcagcat
     1561 cttgatcgcg catgcgcgca gtgccaatcc ggtgagccgg ttgctcacgg aagctttgtt
     1621 accccaggac gacgattatt tcatcctgaa accaaagcac tcagcgttct attccactcc
     1681 gctggatgca atcctctcaa aactcggaac gcgcacagtg attctcgcag ggctcacaac
     1741 ttccgcctgc atcctcttaa cggcaggcga agtgtacgtg cgagacttca aactatttgt
     1801 tccctcggac tgtgtcgccg gtctcaatcg gccggatcat ctcaaggccc tgtcgctcat
     1861 gagaacgagc ttccaagccg acacgaggcc ctatcgcaaa ttgaatctga gatccttggc
     1921 ttcttgaacc gcgacaattc ttgtgacaag cttttcccgc tacacgccac ggtcgtggca
     1981 cttccgaaag gcaaagcatg ccatcacctt cggccgcaaa ccgtattcgc aggatcgtaa
     2041 tcgttgcagg cggcaccggc ggcttggggc gcgctgtggc gctcgctttt ctcagtgaag
     2101 gagacacggt cgctgttccc taccgtaacg aaagcgagtt tgccgcatta aagagccagg
     2161 cgggcgaatc tgctgcggca ttacacggtc aacaggtcga tgtaacagac gagtcgtcgg
     2221 tgaagaaatt catcgatgga gtggtcgctc agcatggcca tattcacgcg ctcgtcaaca
     2281 ccgttggcgg ctacatcggc ggcccaaagc tgtgggaact tgccgacgcc gatctcgatc
     2341 gcatgctcac actgaatctg cgttccggat ttgtcctggc gcgtgcagcc gttcgtgtga
     2401 tgctcaggca aggtaacggc tccatcgtga acgtggccgc acgagcagct ctggaacctg
     2461 cagccggagc atccgcctat gccgcctcaa aagccgctgc ggtcgccatg ttcgattcgc
     2521 tcgccgccga actgagaggc acgggcgtgc gcgcaaattc aatcctgccc agcatcatcg
     2581 acactgaagc gaaccgcaaa gcgatgcccg acgccaattt ttccaagtgg ccgaagccgg
     2641 aagacatcgc gcgagtaatc ctcttcctat gtagcgacga agcaaagctg atccacggag
     2701 ccgcaatccc ggtctacgga aacgcttaat gacgagtcac aatcttaata agcgttcttc
     2761 ggcaaagcgc ggacgatagc cccttgcaaa tccggtgtgg tattatccgc gcgtctccga
     2821 agacataagc accaaatcca gcaatgcttc gccacacgat tgtattgcac gatgctgccc
     2881 tgcggcggag ggcaagaggt tctaactggg aagccaagcg cggagcaaga aattcttcga
     2941 agtttctcgc tccgacggat attgtctcac cgtgataaaa aatctcgatc cggtccggag
     3001 cctcctatga cgatagcgaa agtaactctg gttgccttcc ttagttcact tatgggcctt
     3061 ctcgcggttc cagcatcagc tcagggtcga accgatgcgg ctgctatacg caacatcgtt
     3121 gaaggcgaaa ccgtggcatg gaataaagca gacccggtcg catacgcaag ccacttcgcg
     3181 gcggacggca cattcacgaa catcctgggc gcgttttaca aaggacatga tgccttcctc
     3241 gcagagcaca cccagatatt taagacgatc ttcctcggta gcaccctcca gcaagacatc
     3301 gtctcgctcc agttccctac gccggatgtg gcagtggtgg aggtgcttac ggcagtgacc
     3361 ggagcgaaaa tacccggcgc tcgcagcgat agtttcgatg cgaaggggcg cctgcgcacg
     3421 cgattgttgc aagtgatagc gcgaagaaac ggaaagtggg agattgtcgc ctatcataat
     3481 gtagaggtaa aagacgctct tcacgcaccc gagccgcagt gacgggtcac acagtcaccg
     3541 gtgagccgcg ctgtccagat agagccctag ccaattgggg aaaatggaag atgcagtagg
     3601 cgaaggtact gacttctaag cgtctcgtgt ctgaggggtt agaagattgg ctgggaggcc
     3661 tggactcgaa ccaggataat cagatccaga attgggtcgc cctgccaaaa atcccttgta
     3721 aaatgcggtg aattgggctc actggcccca ttttggcccc atgcaggaat agctctcttg
     3781 gaacgagtgc tattatgctt gatcggttct cgctagtcga gaagctggct cgcccaagag
     3841 tgtggaggcg cggcgcgatg ggtccaagaa aagcacaagc tgagtcaatc cctccaagtc
     3901 tttctccaaa tcaggcgatc ccgctcctta ggcgccaatg cgagcgtgcc gatgcgattg
     3961 ctgcggcaca tttcaatgac ccacaaatag tcgtttggac gaattcgact gagaacatcc
     4021 tgaatcagac gtttgggcaa cctaacggag accggcatcc gaacactaag gctttcatgt
     4081 acgcggatag tggcctgtca atccacgtca acatgtctga tggcgaaatc caaagagatc
     4141 acgtcgcccg aacagaggta aggaaggctc tccttcaggg gtttgtcgaa caacttgaag
     4201 accttgctcc gcctagtgca cgaacggcgc ccgaccgata tacgtttcat tccgagattg
     4261 aacgcgttgg tggcgatctt taccgcaacg gccactacaa gtccgccgca ctcgaatctt
     4321 atatccgcgt cattcgccag gtacgggata tctctcgaat tgacgacgac ggggactctc
     4381 taatgaacaa ggcgttcgcc tgcgacaaac aaaccccggt tatccaattc aataccttac
     4441 agaccgaatc ggagcgtgac gagcagcgcg gtatctattt tctcttcaaa ggtatcgttg
     4501 gactgcgcaa ttccaaggct cacgacaacc gcctgtttga cgatcctcta agggctcatg
     4561 aatatttggc tttggcgagc ttgttgatgc ggctgctcga aattgctcga gtaaacccgt
     4621 tgccctaagg cgcgtttttt ttgccgactt tttggctcaa ttcgggcgca cgttcggtga
     4681 cgcttcggtc cgcttggcct cgacaacgtg aaactcagcc accctttcca tcgcctccgc
     4741 aatatccttt tcgtcggtga tgttgtaccg ctgaaacact gccgtcgtct tgtgtcccga
     4801 aattcgcatc gccactgact gaggcacgcc cgctcgcact aggttgcgaa cgcccgcccg
     4861 tcgcaggtcg tggaagatta ggccgcagta cgtccagcga aacgacttct cacacttggc
     4921 gcagcgcggc gcttcgtcct tcggcagttc agggggccac ggctgtttct ccacctcgcg
     4981 tcggtcatgg cacgtcgggc attcccatag catccggctg acgccactct tggtagtaat
     5041 gctggccttg atgcacgccc gaatccacgc cttacgaaaa ctgcccagcg gtgcaacgcc
     5101 cttccgcgtg aacacgaggc ccgaggcgca tttcgtcgtc ttccaaagct cggccattac
     5161 ctgcgccagt tccggcacct tgccgtacgg aatcgcgcgc ggctcagcat tcttcgtgtc
     5221 ctcggattcg agccgaatcg tttcggtctg atggtcaacg cgcgaccacg tgagattctt
     5281 cagttcccca agtctcatcc cggtgaaata gccgaaggtg tagatgggcc g
//
LOCUS       DATNDT010000172         2908 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3768374, whole genome shotgun sequence.
ACCESSION   DATNDT010000172 DATNDT010000000
VERSION     DATNDT010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2908
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3768374"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            268..2358
                     /gene="pheT"
                     /locus_tag="VN788_05700"
     CDS             268..2358
                     /gene="pheT"
                     /locus_tag="VN788_05700"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HXU20060.1"
                     /translation="MKVVYNWLKEFVEVTAPAAELRSRLSLAGVAIDSVEQTAAGPVL
                     DAEITANRPDCLGHYGIAREVAAIYRVPVKELQPKLKEAAEKTSDVTRVDIETPELCG
                     RYTARVLRGVKVQPSPGWLRQRLEAIGQKSINNVVDVTNYVMFELGQPMHAFDLDKLG
                     EKRIVVRRARTGEKIRTLDGAERTLTKDMCVIADATRAVAIAGVMGGAESEIGFSSRN
                     ILLESAWFDPISVRRTSKALGLRTEASYRFERGADPEMAETASRRAAELIQQMGGGEI
                     LADVVDAYPRREAPIAIELSRLELLRVMGADIPDRDIEEILTALGFEPSRQNSNSGRA
                     ESRETTWKCERPSWRRDVTRGIDLIEEAARHYGYDKFPPRLPPAKQPAHRLPEAQALD
                     RLRERVIALGYQEIVAIPLVDARRDEIFRADGVMPAVIGNPLAEDASVMRSNGVVSMI
                     GALEWNLNHGQRNVRLFEIGKAYALRDGEPTETPVLTLGVTGLASEKTIYEDAREFGF
                     ADLKGDLDRIGELAGALAWRLGGPKWLAGGCGAQISAAQPSEAVTIGVAGQLARRIAD
                     PLKLRQEIFLAELHLEPLLAAVERASAALKFAPIARFPSVERDFSLVLADGVTFAQVA
                     DAIRALGIRELRNIEAADLFRGGAIPTGKYSLMIRAVFQSEDTTLTEAQVAEFSSRIV
                     AALQTKVGATLRAG"
     gene            2566..>2908
                     /locus_tag="VN788_05705"
     CDS             2566..>2908
                     /locus_tag="VN788_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU20061.1"
                     /translation="MKRILVADDDSQTRKSLTEILRDEKFAVSTARDARTALNKLRKT
                     HFDLALLDLGMPKMSGLDVLKALRGKKSLPRMLVITSEDGRPGTVHEHAYQGVSKSIN
                     RETLIKLVREYL"
BASE COUNT          635 a          741 c          991 g          541 t
ORIGIN      
        1 cgggccatcc ggagcatgaa ccgatgctgg ccgagcttca gaggcttttt gatacgcacc
       61 aggaaagcgg cttcgtacgc ttcgaatacg agacgcacat ttactacggg cgattgcggt
      121 cgcagtaatg ctgacggcga cggaaaaata cttactgcga taggagcagc gcacccttcg
      181 ccacaacact cagggcaaga aggagacgct gcgcgtcgcg atttttatgt cggagctgaa
      241 gctccgactc cctatgggcc ggaataaatg aaggttgttt acaactggct gaaggaattt
      301 gtggaggtga cggcgccggc ggcggagctg cggtcgcggc tctcgctcgc gggcgtggcc
      361 atcgattccg ttgaacagac cgcggcgggg ccggtgctgg acgcggagat tacggcgaac
      421 cgtcccgatt gcctggggca ctacggcatc gcgcgcgaag tggcggcgat ctaccgggtg
      481 ccggtgaaag agctgcagcc gaaactgaaa gaagccgctg aaaaaacttc cgatgtgacg
      541 cgggtggaca tcgaaacgcc ggaactttgc ggtcgataca cagctcgggt gctgcgcggc
      601 gtgaaagtgc aaccttcacc gggctggctg cgccagcgac tcgaagcgat cggccagaag
      661 tcgatcaaca atgtggtgga tgtgaccaac tacgtgatgt tcgagttggg ccagccgatg
      721 catgcgttcg atctggacaa gctcggggaa aaacggatcg tggttcgtcg cgcgcggacg
      781 ggagaaaaaa ttcgtacact cgacggcgcg gaacgcacgc taacgaaaga tatgtgtgtg
      841 attgcagatg caactcgcgc ggtggcgatt gccggggtga tgggcggtgc ggagagcgaa
      901 atcggcttca gcagccgaaa tattttactg gagtccgcgt ggttcgatcc gatctcggtg
      961 cggcggacgt cgaaggccct ggggctgcgg acggaggcgt cctatcgatt cgagcgcggg
     1021 gccgatcctg aaatggcgga gacggcttcg cgtcgcgcgg cggagctgat ccagcaaatg
     1081 ggcgggggcg agattctagc ggacgtggtg gacgcgtacc cgcgccgcga agcgccaatt
     1141 gcgatcgaat tatcgaggct agaattgctg cgggtgatgg gcgcggatat tccggatcgc
     1201 gacatcgaag aaattttgac agcgctcgga tttgagccgt cgaggcagaa cagcaattca
     1261 gggagggcgg agtcccgcga aaccacgtgg aagtgcgaga ggccctcgtg gagacgcgat
     1321 gtgacgcgcg ggatcgattt gatcgaggaa gcggcgcggc actacggata cgacaaattt
     1381 ccgccgcggc tgccgcccgc aaaacagcct gcgcaccggc tgcctgaagc acaggcgctg
     1441 gaccgcttgc gcgagcgcgt gattgcgctc ggctaccagg aaatcgttgc gattccgctg
     1501 gtggatgcgc ggcgcgatga gatttttcgg gcagacggcg tgatgccggc tgtgatcggc
     1561 aacccactgg ctgaggatgc ttcggtgatg cggtcgaacg gggttgtgag catgattggc
     1621 gcgctggaat ggaatttgaa tcacggacag cgaaatgtgc ggctatttga aattggaaag
     1681 gcgtatgcgc tgcgcgatgg cgagccgaca gagacgcctg tgctgacgct gggagtgacg
     1741 gggctggcga gcgagaaaac catttatgag gatgcgcgcg agtttggatt tgccgatttg
     1801 aagggcgacc tcgaccgcat cggggaattg gccggggcat tggcgtggcg tctgggtggt
     1861 cccaaatggc ttgcaggtgg ctgcggagct caaatttctg ccgcgcaacc aagcgaagcc
     1921 gtcaccatcg gcgtggctgg gcagcttgcg cgcaggattg ccgatccgct gaagttgcgc
     1981 caagagattt ttctggctga gttacatctg gagccgctgc tggcggccgt tgaacgcgcg
     2041 agcgctgcgc tgaaattcgc gccgattgca cgtttcccgt ctgtagaaag ggacttttcg
     2101 ttggtactgg ccgatggcgt cactttcgcg caggtggcag atgcgattcg cgcgctggga
     2161 ataagggaac ttcgcaacat tgaagccgcc gatctttttc gcggaggcgc cattccgacg
     2221 ggaaaatact cgttgatgat tcgtgcagta ttccagagcg aagacacaac actgacggaa
     2281 gcgcaggtgg cggaattttc gtcgcgaatc gtggccgcgc tgcaaacaaa ggtcggagcg
     2341 accttgcggg ccggctgaaa aatagtcggc cagacgagct gagttcggcc gcccacgcca
     2401 ggttatccct gcgcgtcgcg agcaagagac cttcacggcg gagatatgag ggcgcgatag
     2461 actctcaatg aagacttcca catttaattt actggcgcgg ctaatgcaaa tagagaggcg
     2521 cggaatgaaa acgttcgggc gcaatatcgc atcacgagaa aagcgatgaa acgcatcctg
     2581 gtggctgatg acgattcgca gacgcgaaag tcgctaaccg aaatcttgcg cgacgagaaa
     2641 ttcgcggtat cgaccgccag agacgcgcga actgcgctca acaagcttcg aaagacgcac
     2701 ttcgatctcg ccctactgga cctcgggatg ccgaaaatga gcgggctgga cgtgctgaaa
     2761 gcgctgcgcg gaaagaagtc gctgccgagg atgctggtga tcacgtcgga ggatgggcgg
     2821 cccggtacgg tccacgagca tgcctatcag ggcgtttcaa aatcaatcaa tcgcgaaacg
     2881 ctgatcaagc tggtgcgcga atatctgg
//
LOCUS       DATNDT010000173         3614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3192126, whole genome shotgun sequence.
ACCESSION   DATNDT010000173 DATNDT010000000
VERSION     DATNDT010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3192126"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /locus_tag="VN788_05710"
     CDS             <1..1873
                     /locus_tag="VN788_05710"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HXU20062.1"
                     /translation="IIGVAPPTFSGIEIGIDPDFYLPAPNSGDSPAFVTIVARLKPGI
                     SIAEARASLAPIYQETVRESQLSGREREQDMSGLEIQPIPRGLSRVRDKFGLATQVAM
                     ALVGLVLLIACVNVANLLLARGISRRRELTVRMAIGAGRWRLIRQMLVEAALLGSVGA
                     IGGLLAANWTAKSLAAALSTKELPVQLDAALDVRVFVFAAAVLAITVLLCGLIPAMAA
                     TRGDLTSDLKVAGVNAAHAASRSRLGSLLLAAQISISAIVLIAAGLLLHSLFNLETYN
                     LGFDADHVLALTLTGKDASRALYDQLSDRAKYLPGVKGAAYSVMLPLQGNVVGINVTV
                     PGYTPGSAAETHAYMNGVSPGYFQAMRIPLLAGRDFTPQDGVPQMPGVVIISRNMAEH
                     YFAGKDAVGQEIRIGGSGRPPLRIIGVVADSVYFGVREKTTDVMYTPYGRSTLHTSMM
                     VRASGNPAALASAVTDLIHSMDRSIKIESIETMRHALNDSLHEDRLIGALCGVFAALA
                     LALTCIGIYGVLSFQVARRTNEIGIRMALGAHRCDILSLILLRGLRILIAGLAIGIVG
                     ALAITRVMAHMLFGLSPSDFFTYAAVALVLLSAALAALYVPARRAIRIDPMVALRYE"
     gene            complement(2849..>3614)
                     /locus_tag="VN788_05715"
     CDS             complement(2849..>3614)
                     /locus_tag="VN788_05715"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HXU20063.1"
                     /translation="DAASSLGIHHYWWGTFRFDETQPYWPQLDALKPKVEKIAKLNEK
                     YGIKGMAHPRDGATSVSGAFFDLLYVLKNFDPRWVSFQYDTHHLLQAFDSGWVQQLRL
                     GTPYIGGFVWKDVMIEPANSPNPDDEWRAAHPRPAPAAGAGAEGFGGRGGGRGRGRGG
                     FGGGRGRGGNPSAPTKTRIRQVPVGTGMINLALIAQTLKEIGFNGPMECEPEWPQLGG
                     ADQGATELSIPREQAIALLKRDYDTMTGIMSGAGLV"
BASE COUNT          683 a         1100 c          997 g          834 t
ORIGIN      
        1 gatcatcggc gtcgcaccgc ccaccttttc cggcatcgaa attggaatcg atccggattt
       61 ttaccttccg gcgccgaata gcggtgactc acccgctttc gtcacgattg tggcgcgcct
      121 caagccggga atttcgatcg cagaggcgcg cgccagcctc gcgcccattt atcaggaaac
      181 cgttcgtgaa tcccaattat ccgggcgaga aagggagcag gatatgtccg gcctggagat
      241 ccagccgatt ccacgcggcc tctcgcgcgt gcgcgataag tttggtctcg ctacacaagt
      301 cgccatggca ctggtcgggc tagtccttct gattgcttgc gtcaacgttg cgaaccttct
      361 gcttgctcgc ggcatcagtc gcaggcgcga attgactgtc cgcatggcga tcggcgccgg
      421 ccgctggcgt ctgatccgcc aaatgctcgt cgaagccgcg ctactcggtt cagtgggagc
      481 gataggcggt ctgctcgcag ccaattggac tgcgaaatcg cttgcggctg cgctctccac
      541 aaaagagctt ccggtgcaat tggacgctgc tctcgacgtg cgcgtgttcg tattcgccgc
      601 ggccgtcctc gcgatcacgg ttctgctttg tggcctgatt cccgcaatgg ccgccacgcg
      661 cggcgatctc acctccgatt tgaaagttgc aggtgtgaat gctgcgcatg ccgcatcgcg
      721 ctcgcgcctc ggcagtctgc tgctcgctgc gcaaatctcg atttccgcga tcgttctcat
      781 cgccgcaggt ctgctcctcc acagcctctt caatctcgaa acctacaatc tcggcttcga
      841 cgccgatcac gtcttggccc ttacgttaac cggcaaagac gccagcaggg ctctttacga
      901 tcagttgtca gatcgcgcga agtatcttcc gggtgttaaa ggagccgcct attccgtgat
      961 gttgccgctg caaggcaacg tcgtgggaat taacgtgacc gttcccggtt atacgccggg
     1021 ttctgccgcg gaaacccatg cgtatatgaa cggcgtttct cccggatact tccaggccat
     1081 gcggattccc ctactggcag gccgcgattt cacgccgcag gatggcgttc ctcagatgcc
     1141 cggtgtcgtg atcatcagcc gcaatatggc ggagcactac ttcgcgggaa aggatgcggt
     1201 cggccaggag atcagaatcg gaggaagcgg gcggccacct ctgcggatca ttggcgtggt
     1261 cgcggactcc gtttacttcg gtgtccgcga gaaaaccaca gacgtaatgt acacgccgta
     1321 tgggagaagc acccttcaca ccagcatgat ggtgcgcgca agcggcaatc ccgcggcgct
     1381 cgcaagcgcg gtcaccgatc tgatccactc tatggatcgt tcgattaaga tcgagtccat
     1441 cgagaccatg cggcatgcgt tgaacgattc tctccatgaa gatcgcctga tcggcgcgct
     1501 ttgcggcgta tttgccgctc tggcgcttgc cctcacgtgc atcggaatct atggcgtgct
     1561 gtcattccag gtcgctcgcc gcacgaacga gatagggata cgcatggcgc ttggcgcgca
     1621 ccggtgcgac attctctcgc tcatactgct gcgcggattg cgaatcctca tcgctggcct
     1681 cgcgatcggc atcgtcgggg cgctcgcgat cacccgcgta atggcccaca tgctcttcgg
     1741 gctctcgcca tccgatttct tcacgtatgc ggctgtcgcg ttggttttac tttccgccgc
     1801 actcgcggcg ctctatgttc cagcgcggcg cgccatacgc atcgacccaa tggtggccct
     1861 gcgttacgaa tgaacaatca atgcccaagc caggtgatgc gatgtttgcc tttgaatttg
     1921 tttttgtagc ggccggcctt gtggtttagt cgggcagttt ttgccgttgt tgttgaattt
     1981 gcttttgttt ggcttttgta gattctctct tcgcgtacaa gcggccaccg ttgcctgcgg
     2041 tgtttgcagg cgcttctcgg tgggcgtttt tgccgttggc tcgtgtaggt gatgtggctt
     2101 ttctgactgg attctggctt cccgcgtacg gagaaatccg ggacagcgac ctgtgccatt
     2161 tttctgcgtg cgtgccctgg gcgcctgctg tgccccaaat tcccgcacag gggcatccgt
     2221 ggctgctccg cgcccgcgcg tgcttcccta aaacatcgga ggaagtgccc ggtttccccg
     2281 ccttcggccc tgcgcatgaa taacaaaatt aagctcgttt tacgctgcca ttcgcgccat
     2341 tttgtgtcca atttgtttgt tttaaagcgc caatggcgca ctttttaacc agcgcccacc
     2401 acgcgccgag atactcatct cttcgagagt gtctacaaac tgacacacaa gaaaccctaa
     2461 aaaagtcctt gcaagtatct ttaacgtgtg gtaaacttgt gtctggagtt tgcgttactg
     2521 acgtcatgac aacagccgcc acagtccaaa gccgaagccc tgctagcccc ttgctactat
     2581 tcaataatca cagcctttct aatcgcaacc gcaaagaatt aaaaaccggc gtaactcctt
     2641 gcaaacaaag gacagcgccc agttctaatc gcagtagcca gcgggagaaa gcgaagaggc
     2701 gaaagagcga aatccacgtc ggcaatgatg cagccgccat gcattttcta atcgccagtc
     2761 tgccgattag aatcgaccgt aacccttgca ccctcaatac aaatcacatt tctaatcgac
     2821 aaaaaaaggg gggtttttag cgtaaaaact agaccagtcc tgcgccggac atgatgcccg
     2881 tcatcgtgtc gtaatcgcgt ttcagcagcg cgatggcctg ctcgcgtgga atcgaaagtt
     2941 cggtcgcgcc ctgatcggcg ccgcctagct gcggccactc cggctcgcac tccatcggcc
     3001 cgttgaagcc gatctccttt agagtctgcg cgatgagagc caggttgatc atgccggtcc
     3061 cgacaggcac ctggcggatg cgggtcttcg tgggagcgct tggattgcct ccgcgacctc
     3121 ggccacctcc gaaaccgccg cggcctcgac cacgcccgcc accgcgaccg ccaaatcctt
     3181 cggcacctgc gcccgcggcc ggtgcgggcc gcggatgcgc cgcgcgccat tcgtcatcgg
     3241 gattcgggct gttggccggt tcgatcatca cgtctttcca gacgaacccg ccgatgtagg
     3301 gcgttccgag gcgcagttgt tgcacccagc cgctatcgaa ggcttgaagg agatgatgcg
     3361 tgtcgtattg aaagctcacc caacgtggat cgaaattctt cagcacataa aggaggtcga
     3421 aaaaagcgcc tgatacgctc gtggcaccgt cgcgcggatg cgccatgccc tttatcccat
     3481 acttttcgtt gagcttggcg attttttcga ccttcggctt cagcgcgtca agttgcggcc
     3541 aataaggctg cgtctcatcg aagcggaacg ttccccacca atagtgatga atgcccagcg
     3601 acgatgcagc gtcc
//
LOCUS       DATNDT010000174         3524 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1307854, whole genome shotgun sequence.
ACCESSION   DATNDT010000174 DATNDT010000000
VERSION     DATNDT010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3524
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1307854"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VN788_05720"
     CDS             <1..1163
                     /locus_tag="VN788_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20064.1"
                     /translation="VAVPTALVSARQVVATVPANMLAQPRRYIVQVQNPSLAASNVAG
                     LTVVESVAVGSAPVGIAVDTDRDLAYVANSGDGTLSLVSLASPSPGLSPQSLGTAGTV
                     IGAPITVGTTPAGVAAISRFGMAIVANNGSNNASVVNVAAGIPVPAPISIPLCSACTA
                     PAGVAINQDTGIGAIADTNPSTSSSGTGDVSLLTLPATSSSTASTVAVDQDPVALAVD
                     PTLDFAGVVTASATSSMQFIEMSSGAIAGSVRTGLDNPAGIVFDPVNQVFLAANSLLN
                     SIVIVDPATFIQTPSPVGIGPTSIDYNFQTSTLVTVNSVSHTMSVMAYACPPVPSAPQ
                     CLGPRVRTVLGLGGTQISSPLLGPNAVAVDPKLNLGVLADEDNNRVLLVPLP"
     gene            1353..1967
                     /locus_tag="VN788_05725"
     CDS             1353..1967
                     /locus_tag="VN788_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HXU20065.1"
                     /translation="MKMRAAVPVLAALLFTPSVWAQAASAPAKVGVINVQAAIAGTAE
                     GKQAVAQLQSQFAPRTSELQNMQKQIEDVRARLQAGETTLSDEEKGRLTREGDQLTRS
                     FQRKQQEFQDDSNDAQQDLINRIGRKMVEVLDKYAKDNGFTVVLDTSSQQTPVIYASN
                     QVDVTQEIIHLYDQSYPVKAGAASTTPSRPAPKSAAPKPATPKP"
     gene            2202..2663
                     /locus_tag="VN788_05730"
     CDS             2202..2663
                     /locus_tag="VN788_05730"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXU20066.1"
                     /translation="MALSVGTAAPEFSLKDQNQKEVKLSDYRGKKNVVMVFYPLDWSP
                     VCTNEHVCLVNDMKQFDKLDAQVLGLSVDSVWSHKAFAEKMGIQYPLLADFQPRGAVA
                     EKFGVYLADKGITGRAIAIVDRKGNVAWFKNYDIPVVPDIKEVSEALAKVQ"
     gene            complement(2984..3457)
                     /locus_tag="VN788_05735"
     CDS             complement(2984..3457)
                     /locus_tag="VN788_05735"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXU20067.1"
                     /translation="MNGEQSRAPHARAPDGPSKSGLRKLGIIALFSVLGISMLVIVYL
                     ARNWSVMAKARKLQNPVPSTAEALAAGKESYRQHCQSCHGERGDGKGDKAPELSVAPG
                     DFTDVRVMHRFADGELYWQITYGRKPMPEFGSKLSEEKRWELVDYIRTFANQPER"
BASE COUNT          737 a         1076 c          961 g          750 t
ORIGIN      
        1 atgtcgccgt gcccactgcg cttgtgtctg cgcggcaagt cgtcgccacc gtgcccgcca
       61 acatgctcgc gcagcctcgc cggtatattg tccaggtgca gaatccctcg ctcgcagcct
      121 cgaatgtcgc cggccttact gtcgtcgaat cagttgccgt ggggagcgcg cccgttggca
      181 tcgcagtcga taccgatcgc gatctcgctt atgtcgcgaa ttccggagac ggcacgcttt
      241 ctctggtttc gctcgcctcg ccgtctcccg ggctttctcc gcaatcgctc ggcacggcag
      301 gcacggtgat cggcgcgcca atcaccgtgg ggaccactcc cgcgggtgtc gctgccatct
      361 cacgctttgg gatggccatc gtggcgaaca atggcagcaa caatgcttct gttgtgaacg
      421 tcgcggcggg aattccggtg cccgcgccga tttcaatacc gctgtgctcc gcttgcaccg
      481 cgcccgccgg agttgccatc aatcaagaca ccggcatcgg tgcgattgcc gataccaatc
      541 ccagcacctc gtcttccggc accggtgatg taagccttct cactcttcca gctacgtcat
      601 cgagcaccgc ttccacggtg gctgtcgatc aagatcccgt ggcgctggca gtcgatccga
      661 ccctggactt cgccggcgta gttactgcct cagcgaccag cagcatgcag ttcatcgaaa
      721 tgtccagcgg ggccattgcg ggaagcgtcc gcacgggtct tgataatccc gccggaatcg
      781 tttttgaccc cgtgaatcaa gtgttcttgg ccgcaaatag cctgctgaat tcgattgtga
      841 ttgtggaccc agccacgttt atccaaacac cgtcgcccgt gggaattggg cccacgtcga
      901 tcgattacaa ttttcagacc agcacgctgg ttacggtgaa ttccgtaagc cacacgatgt
      961 cagtgatggc ctatgcctgc ccgcccgtcc cgtcggcgcc ccaatgcctg gggccccgcg
     1021 ttcgtacggt gctggggctg ggagggacac aaatctcctc acccttgctc gggcccaatg
     1081 ctgtggctgt cgatccaaaa ctcaacctcg gcgtcctcgc ggacgaggac aacaatcgcg
     1141 tcctgctcgt tccgttgcct tagtgacgtg gctggcagcc tggtgcggca accccgccga
     1201 agaggcgagc atcggaatgt cagttaaatg ttaaaagagt gggttccacc ggccagaacg
     1261 gccaaatcac gttgaccatg ccgccgaata gtggctataa tttgcggctc cccattctga
     1321 aaatttaacg gtaattttgg caaggagtaa ggatgaagat gcgagcagca gtaccggtct
     1381 tggcggcgct attgtttaca ccctcggtgt gggcgcaagc agcctccgcg ccggcgaaag
     1441 tgggcgtcat caatgtgcaa gcggctatcg cgggaacggc ggagggcaaa caggcagtcg
     1501 cccaattgca gtcgcagttt gctccgcgaa ccagcgaact gcagaacatg caaaagcaga
     1561 tcgaagacgt gcgcgccagg ctccaggccg gcgagacaac cctgagcgac gaagaaaagg
     1621 gccgtctcac gcgggaaggc gaccagctaa cccgcagctt tcagcgcaag cagcaggagt
     1681 ttcaggacga ttcgaacgac gcgcagcagg atctcatcaa tcgcatcgga cggaagatgg
     1741 tcgaggtcct tgacaaatat gcgaaggaca acggctttac cgtggttctc gatacctctt
     1801 cccagcagac gcccgtgatc tatgcctcga atcaggtcga cgtgacccag gaaatcattc
     1861 acctctacga ccagagctat ccggtgaagg ctggggccgc atcaaccact ccttcgaggc
     1921 ccgcgccaaa atcggctgct cccaaaccgg caactccaaa gccctagatc gctggcaaac
     1981 attcttcgct ttcgactttg aatttgcggg tgccccactt gccctgagca ccgccgaagg
     2041 gtgctcgttt tgtgagcgtg gggtttttcg cttcttgcgt ttcgcattcg caatcattca
     2101 aacctcttca aacgcaattt gtccccttcg accgcttcgg ctatcatcaa ataatcgcgt
     2161 tcgatgtcac ggattatgcg cgaacaatca ggaggattgg aatggctctt tcagtcggca
     2221 cggcagctcc ggaattttcc ttgaaggatc agaatcagaa agaagtgaaa ctctccgatt
     2281 atcgcggcaa gaaaaatgtg gtgatggtgt tttatcctct cgactggagc cccgtctgca
     2341 ccaacgagca tgtttgcctc gtcaacgata tgaagcagtt cgataagctc gatgcgcaag
     2401 tgctcgggct cagcgtggat agcgtgtggt cccacaaagc ttttgccgaa aaaatgggca
     2461 tccagtatcc attgctcgcc gattttcaac cgcgcggcgc ggtagccgaa aagttcggcg
     2521 tctatctcgc cgacaaaggc atcaccggac gcgccatcgc catcgtggac cgcaagggca
     2581 atgtcgcttg gtttaagaat tacgatatac cggtcgttcc agacataaag gaagtcagcg
     2641 aagccctcgc caaggtccaa taacctttat cagcagttgt agtcgcacgg catgccgcgc
     2701 ccgccgaaca cgcttagctc agggccgcac cgcgtccgcc acctccgagc ggccgtacgg
     2761 gcgtagcacc gctacgcccg ccaaccatgc ttggcgttat gtccgccatc caaagcggct
     2821 aaataggcac cacagtcatt ccgcttgccc tgacgaagga agggagcgaa gcgtcccgcg
     2881 cttttgtatt tgcgcgaagc gccgggacgc gcagtcgagg aatctctctt cgattcaacc
     2941 agtaatagca gttaacccga agaaatctct cgttcaaacg tgttcagcgc tcaggttggt
     3001 tcgcaaatgt ccggatgtag tccacaagct cccagcgctt ttcctcgcta agcttgcttc
     3061 caaactcagg catcggtttg cgcccgtaag taatctgcca ataaagttcg ccgtcagcaa
     3121 agcggtgcat cactcgcaca tcggtaaagt ctcccggagc caccgaaagt tcgggagcct
     3181 tatcgccctt cccatcgccg cgttctccgt ggcaactttg gcagtgttgc cgatagcttt
     3241 ccttgcctgc ggcaagggct tcagcggtgg acggcacggg attttgaagc ttccgcgcct
     3301 tcgccatcac gctccagttc cgcgcaagat aaacgatcac cagcatgctg attcccaaaa
     3361 cactaaataa tgcgatgatg ccaagtttgc gaaggccgct tttcgacggg ccatcgggcg
     3421 cgcgcgcgtg cggcgctctt gattgttccc cgttcattta gctgcacagg attttctcac
     3481 aggccgcttg cgatcggcgg cgcgaattgc ccgaaataac cgcc
//
LOCUS       DATNDT010000175         2311 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3834596, whole genome shotgun sequence.
ACCESSION   DATNDT010000175 DATNDT010000000
VERSION     DATNDT010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3834596"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="VN788_05740"
     CDS             <1..1360
                     /locus_tag="VN788_05740"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HXU20068.1"
                     /translation="KSLVAKEGRIPKADLERVAERIFKLSEADETEVEIGAVTDALTR
                     FANNTIHQNVAEQTLAISVRAVVDGRTARATTNKTDDESLGRVTAAALALARNQPKNP
                     DLLPMHRSPKYQKVARFFPATAAASPQDRARAVTRVCRMVEKAKQTAAGIFSSGSSQS
                     LLANSRGLSAHYEQTRSEFSVTVLESKSSGWAKANSPNIRNFDPDKLAERASSTCAES
                     REPKEITAGHYTTILPPSAVLDLVGFLFYDFAGTAVLDKRSCLTGRMGKKLFGDNISI
                     WDDAYHPLQTGPPYDGEGMPRQKVTLVDRGTPTNLVYARATAKKMKAKPTGHGFSLPN
                     EYGEAPMNLVFAGGDKSMEEMIRTTERGVLVTRLWYIREVDPYAKVLTGMTRDGTFLI
                     ENGRVAGGLRNFRFNQSIIEMLANVEMLGPAVRAAGEESFEMVVPTMKVRNFHFSEVT
                     KF"
     gene            1494..1988
                     /locus_tag="VN788_05745"
     CDS             1494..1988
                     /locus_tag="VN788_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXU20069.1"
                     /translation="MANLARRESIFNDLFDFRRDFDQMFNRLLRTGSERGEHAGQTSA
                     DTFLPAVNAYIDKDGRKFVCQVALPGIEPKDVNIQVQGNVLSIRGERTLSNESKEANY
                     VHSELTYGSFERNILLPEGVDPDKLAAEYRNGVLEVTAPVSAAALPRRIEIKATESAK
                     RASA"
     gene            complement(2195..2269)
                     /locus_tag="VN788_05750"
     tRNA            complement(2195..2269)
                     /locus_tag="VN788_05750"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2234..2236),aa:Gln,seq:ttg)
BASE COUNT          546 a          651 c          662 g          452 t
ORIGIN      
        1 aaaatcatta gtggccaagg aaggccgcat cccgaaggcc gatctcgagc gcgtcgcgga
       61 gcgtattttc aagttgagcg aggcggacga gactgaagtc gaaatcggcg cagtgaccga
      121 cgcgctcacg cgcttcgcca ataataccat tcatcaaaat gttgccgagc agacgctcgc
      181 aatctcggtg cgagccgttg tggatggtcg cacggctcgc gccacgacca acaaaacgga
      241 tgacgaatca cttggacgcg tgacagcggc cgctctggct ttggcgcgca accagccgaa
      301 gaatcctgat ttgctgccga tgcaccgctc gccaaaatat caaaaggttg cgcgcttttt
      361 cccagctacc gccgctgcat cgccccagga tcgcgcccga gccgtcaccc gcgtgtgccg
      421 catggtggag aaagccaagc agactgcggc gggaattttt tccagcggat catcgcagtc
      481 tttactggcc aattcgaggg gactttcggc gcattacgaa cagacgcgct cggaattttc
      541 ggttaccgtg ctcgaatcga aatcttccgg ctgggcgaag gcgaattcgc ccaacattcg
      601 caatttcgat cctgacaagt tagccgaaag agcgagcagc acttgcgccg aatcgcgcga
      661 gcccaaggaa attaccgcgg gccactacac gacgatcctg cctccctccg ccgttctcga
      721 tctcgtcggg ttccttttct acgatttcgc gggcactgcg gtgctggaca agcggtcgtg
      781 cttgaccggc cgaatgggca agaaactttt tggcgacaat atcagcattt gggacgacgc
      841 gtatcacccg ctgcaaaccg gcccgcctta tgacggagag ggaatgccga ggcagaaagt
      901 cacgctggtc gaccgcggca ctccgacaaa tctggtttac gcgcgggcca cggcgaaaaa
      961 aatgaaggcg aaaccgacgg gccacgggtt ttccctgccc aatgaatacg gcgaagctcc
     1021 catgaattta gtatttgcgg gcggcgacaa atccatggaa gaaatgatcc gcacgaccga
     1081 acgcggcgtt ctggtcaccc gcctgtggta tatccgcgaa gttgatccat acgcaaaagt
     1141 gcttacggga atgacgcgcg acgggacgtt cctaattgaa aatggacgcg ttgcgggcgg
     1201 ccttcgcaat ttccgcttca atcaaagcat tatagagatg ctggcaaacg tcgaaatgct
     1261 ggggcccgcg gtccgcgcgg ccggagaaga gtccttcgag atggttgttc cgaccatgaa
     1321 agtacgtaac ttccatttca gcgaggtcac aaaattttaa ctgaatttct tcggtaccca
     1381 cttgacaagc aattgtgccg gacggagact cgcggaagct ggagccaatc cggcaagccg
     1441 cgaccgtaac gcccgccccg cgatcgccac gaaaatcaaa taggaggtag ccaatggcca
     1501 atctggcccg tcgtgaaagc atcttcaacg atctcttcga tttccgtcga gacttcgatc
     1561 aaatgttcaa tcgtcttctg aggaccggct cggagagggg cgagcatgcc ggtcaaacaa
     1621 gcgccgacac gttcctgccg gcggtcaacg cgtacatcga taaggatggc aggaagttcg
     1681 tctgccaagt tgcgttgcct ggcatcgaac cgaaagacgt gaatatccaa gtgcagggga
     1741 atgtgctctc gatccgcggc gagcgcacgc tcagcaacga aagcaaagag gcgaattacg
     1801 ttcacagcga gctgacatac ggctcgttcg aacggaatat tctactcccc gagggcgtgg
     1861 atccggacaa gctcgcagcg gagtatcgca acggcgtgct cgaagtcacc gctcccgtct
     1921 cagcggcggc gttgccacgc agaatcgaaa taaaggcgac cgaaagcgcg aagcgcgcca
     1981 gcgcatagaa aaatctccta ctttgactga aggcccggaa ctccgcggtt ccgggccttt
     2041 cagtttggct acgagatagg agagtgttgc gcctgagcct gttcgagatt cttgtcagcg
     2101 tgccaggtgg tgccagctgt gccgcaaggc agttcggttt tgaaattttt cgaacgcggg
     2161 aagcttcacg gaaggttcgc aaaatcgcaa ggaatggctc gggggcttgg actcgaacca
     2221 agattctagg ctccaaaggc ccatgtgcta ccaattgcac cacccccgag tagcgcagaa
     2281 agtttagcag agatcggccc ggtttttctt t
//
LOCUS       DATNDT010000176         4972 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3749870, whole genome shotgun sequence.
ACCESSION   DATNDT010000176 DATNDT010000000
VERSION     DATNDT010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4972)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4972)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4972
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3749870"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VN788_05755"
     CDS             complement(<1..730)
                     /locus_tag="VN788_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXU20070.1"
                     /translation="MTAPAQANDTSVAAITRRARKASRALAKLSADARVEVLMAAAQA
                     IEDARQRILDANERDRHAAEPAVTSGKMTASMLARLRVTDAGVAQMAAQIREVARLED
                     PIGRKLSAAELDDGLVLYKESCPFGVVGVIFESRPDVIPQVASLCLKSANAVILKGGS
                     EAAETNQELVAIWRECLIRFRGVPADSINLLESRADVMEFLTEDRDVDLVVPRGSYQL
                     VKHIMQHSRIPVLGHGEGICHVYVD"
     gene            complement(739..1926)
                     /gene="proB"
                     /locus_tag="VN788_05760"
     CDS             complement(739..1926)
                     /gene="proB"
                     /locus_tag="VN788_05760"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HXU20071.1"
                     /translation="MESDHKLETERAHLLRSARRVVIKIGTATVTEPEGNLCLERVEP
                     VVRSIAALMKERRQVVLVSSGAVGLGRGWLGLHWSRLEDMVTKQACAAVGQSLLMDAY
                     KSLFSRWGIKIAQVLLTEEDFTNWRRYSNLQHTIEKLLGFGVLPIVNENDTVSTAELE
                     PVASESRAAAFSDNDRLAALVMSGLEADALVLLTNVDGLLEQSRNGARSNKEGEVIPL
                     VAEMTPEIQALGAGPSESGRGGMRTKLEASEIAMKCGSIAVIANGGRPDTLEQIFAGE
                     STGTAFLPAKRMPGKRRWIAYAANVRGRVVVDAGAQKAITQGKASLLTSGIVRVETRF
                     ASMDVVSIVDSEGREFARGIAKYASDQAEKLSAKRGATAGSKTSSSPVLVTRDNIVLL
                     HKT"
     gene            2160..3038
                     /locus_tag="VN788_05765"
     CDS             2160..3038
                     /locus_tag="VN788_05765"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HXU20072.1"
                     /translation="MLQDRRQHQRVIPNQPLFVRWGQSESVLLSDLSEEGLAIDGIAP
                     NSSAAIIPLAFDLPGGRGHIRTAGEIAWTNDSQNRAGLRFVDLAGTCRQQLREWISAS
                     VFYTPELAVANQDSTQSFCLADETGHAGLPGSDDEDSSRQSMSAYLMLFALGALSLFS
                     GVVFARHYLGGPGDQQIKEITARSELSEISPAAPIAPANSSHATTRFLPPTLPLSVPG
                     LVLQVAAMQHEGNADALAKTLQKRNFPAFVFQRGANAFYRVAVGVYGDPSSAVRVKDQ
                     LEAQGFQAILKRWSPV"
     gene            3132..4370
                     /locus_tag="VN788_05770"
     CDS             3132..4370
                     /locus_tag="VN788_05770"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HXU20073.1"
                     /translation="MLKTGYLLTKAARLAIPFAIVCAMCAPALPQPPSIIITLTGQSM
                     IRSDIRATEPAAVPVIKGLLKGDVVFTNLEAAVAENGETVREGRGFLTPPAALDALKA
                     FGFNLLSLAGNHAFDLKVPGIQNTIREADLRHIVHAGTGNTVAEAVAPGYLRTPKGTI
                     ALIATASGLISPGASATADRPGVNELRVEAGDKENEATADLPGAPANTPNPEDSARIL
                     QSIRYASRHADLVIVYQHNHVFGNHSFSTIFTEGMPERLAPNDWLRKWTHAEVDAGAD
                     IVVMHGAPLLHGVEIYRGRPILYDLGNFIYNVPPTLTYIDEPMNWESAVAYVQFQRKK
                     LQSISFRPIVLNNAGQGQPDIHNEYANNQFLDTRGLPSPATGARAGYILQRLADSSKP
                     FGTTIEVKGNIAEIILKPGG"
     gene            4655..4909
                     /locus_tag="VN788_05775"
     CDS             4655..4909
                     /locus_tag="VN788_05775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20074.1"
                     /translation="MSKTIQVEIEYHDRNSGPTVAGKARVEAGSLPVGIGKALREVWK
                     TMDRKVRFDAAKNGLTIKARVLDASATIETPSDTKQVGEQ"
BASE COUNT         1033 a         1572 c         1316 g         1051 t
ORIGIN      
        1 gatcgacata cacgtgacaa atcccctcgc cgtgtcccag cacgggaatt ctgctgtgct
       61 gcatgatgtg cttcaccaac tggtacgagc cgcgaggaac aacgaggtcg acgtcgcggt
      121 cctccgtcaa aaattccatt acatcggcgc gggattcaag caaattgatc gagtccgccg
      181 ggacgcctcg gaatcgaatc aagcattccc gccagatggc gacgagctct tgattcgttt
      241 ccgccgcttc ggaaccgccc ttcaggatca cggcattggc cgatttcagg cacaacgaag
      301 cgacttgcgg aatcacgtcc ggccgcgatt cgaaaatcac ccccaccacg ccaaaagggc
      361 acgattcttt gtacagcacc aatccgtcat ccaactccgc ggcggagagc ttccgcccga
      421 taggatcctc gagacgcgcg acttctcgaa tctgcgcggc catttgcgcg acgccagcgt
      481 ccgtcacgcg taatcgcgcc aacatcgacg cagtcatttt tccggacgtt accgctggct
      541 ccgcggcgtg gcgatcgcgc tcattggcat cgagaattcg ctgccgcgcg tcttcgatcg
      601 cttgtgctgc cgccatcaga acttccacgc gagcatccgc cgacagctta gccaaggccc
      661 gcgaagcctt tctggctcgc cgcgttatcg ccgcgacaga cgtatcgttc gcttgcgccg
      721 gtgccgtcat tggctggctc atgttttatg cagcaacacg atattatcgc gcgtcactaa
      781 caccggcgaa gagctggttt tgcttccagc cgtcgcgcct cgtttagcgg aaagtttctc
      841 cgcttggtcg cttgcgtact tggcgatacc cctcgcgaat tcgcgcccct cggaatcgac
      901 gatgctcaca acgtccatgg aggcaaagcg agtttcgacg cgcacgattc cagatgtcag
      961 caggctcgct ttcccttgtg taatcgcctt ttgggcaccc gcgtcgacca ccacgcggcc
     1021 gcgaacattc gccgcatatg caatccagcg ccgtttgcct ggcatccgct tggcgggcag
     1081 gaacgcggtt cccgtacttt cgcccgcaaa aatttgctcc agcgtgtccg gccggccgcc
     1141 atttgcaatc acggcgatac tgccgcattt catggcaatc tccgaggcct cgagcttcgt
     1201 ccgcattccg ccgcgtccgc tttccgaagg acccgctccc agcgcctgta tctccggtgt
     1261 catctccgcg accaacggga tcacctcgcc ttctttgttg cttcgggcgc cgtttcgcga
     1321 ttgctcaagc aatccatcga cgttggtgag tagcactagc gcatccgcct ccagtccgct
     1381 catcactagc gccgccaggc ggtcattgtc gctgaacgcc gcggcgcgcg actcggatgc
     1441 caccggttcc aattccgctg tggaaaccgt atcgttttca ttcacgatcg gcagtactcc
     1501 aaaccccagc agtttctcta tagtgtgctg aagatttgaa tatcgccgcc agttcgtgaa
     1561 gtcttcctcc gttagcagaa cctgcgcgat ttttattccc catcgcgaaa acagactttt
     1621 gtacgcatcc atcagaagac tctggcccac ggccgcgcag gcctgcttcg tcaccatgtc
     1681 ttctaagcgc gaccagtgta gacccagcca gccgcggcca agccccaccg cgccggaaga
     1741 aaccagcacg acctgcctcc gctctttcat taaggcggcg atcgaacgaa cgacaggctc
     1801 gacccgttcc aggcacaggt tcccctctgg ctccgtcaca gtagccgtac cgatcttgat
     1861 tactactcgc cgagctgatc gcagcaggtg cgcgcgttcc gtttctagtt tgtggtcgct
     1921 ttccactcga aactccctct gcgccgcgct tatccgccac ggtgatcccc aattgtatcc
     1981 aaatcacggc aggcatgccc tattttgagg attgccgtcc cgctggcaca tcagcctgtg
     2041 gcccagtagc gctctacacg ttgcccgttt gaacaattct cctcatcaga ccgaacccgt
     2101 tcggcaaaag ttcggattac tatcgccccc aagttcttag cctctcactg gggagccata
     2161 tgctccagga ccgccgacaa catcagcgcg tgattcccaa tcagccgtta tttgttcgct
     2221 ggggtcagtc cgagagcgtc ttactctccg atctctccga agaaggcctg gccatcgacg
     2281 gtattgcccc caacagctca gccgcaatca tccctctggc atttgacctg ccgggcggtc
     2341 gcggtcatat ccgaaccgca ggggaaattg cttggacgaa tgactcgcag aatcgcgccg
     2401 gattgcgctt tgtcgatctg gccggcactt gccggcagca gttgcgggaa tggatatctg
     2461 ccagcgtctt ctatacgcct gagctagctg ttgccaacca ggactctaca caatcgttct
     2521 gtctcgccga cgaaacaggc catgcgggtc tccccggaag tgacgacgag gattcgagtc
     2581 gccaaagcat gtcagcctat ctgatgttgt tcgctctcgg cgcattgtct ctattttccg
     2641 gcgttgtgtt cgcgcgtcat tacttgggcg gcccaggcga tcagcagatc aaagagatca
     2701 cggcccgaag tgagctttct gaaatttccc cggcagcacc aatcgcaccc gctaattcgt
     2761 cgcacgcgac aacccgcttt ctccctccaa cgcttccgtt gagcgttcct ggtttggtgc
     2821 ttcaagtggc tgcgatgcag catgagggca atgcagacgc gttggcgaaa acgttgcaga
     2881 agagaaattt tcctgcgttc gtgttccagc ggggtgccaa cgccttctac cgggtggccg
     2941 tcggagttta tggcgatccc agttccgctg tcagggttaa ggaccaactc gaagcgcagg
     3001 gcttccaagc catcctcaag cgttggtcac ccgtataaaa ccgttgtgcg accatatggt
     3061 gcggcgatac cttatgatgc cgttagcctt atctgcggct agaagcctca ctatcgatgg
     3121 tcgcttagaa aatgttgaag acagggtatc tattgacgaa ggcagcgcgc ctagcaattc
     3181 cgttcgcgat cgtctgcgcg atgtgcgctc ccgcgctgcc gcaaccgcct tccataataa
     3241 tcaccttgac gggccaatcg atgatccggt cggacattcg cgccactgag cccgcagcgg
     3301 tgcctgtgat aaagggcctg ctgaagggcg acgttgtctt cacgaatctg gaagccgcgg
     3361 tcgccgaaaa tggcgagaca gtacgcgagg gcaggggatt tctcaccccg cctgcagcgc
     3421 tcgacgctct caaggcgttc ggctttaact tgctgtctct cgccggcaac cacgcattcg
     3481 atctcaaagt gccgggcatt cagaacacca tccgcgaggc ggacctccgg cacatcgttc
     3541 atgccggcac gggaaacacg gtggccgaag ccgtcgcgcc aggttatctg cgcacgccga
     3601 agggcaccat tgccctgata gcgaccgcct caggcctgat ttcgccggga gcgagcgcca
     3661 ccgcggaccg tccgggggtg aatgagcttc gcgttgaagc cggcgacaag gaaaatgagg
     3721 ccactgccga tctgcccggc gcgcccgcca acacgcccaa tccggaggat tcagcgcgca
     3781 tccttcaaag cattcgctat gccagccggc acgccgatct cgtgatcgtc taccagcaca
     3841 accatgtctt cggcaatcac tccttctcaa cgattttcac tgaagggatg ccggagcggc
     3901 ttgctcccaa cgattggctc cggaaatgga cgcatgcgga agtggacgcc ggggccgaca
     3961 tcgtggtgat gcatggcgcg cctctgctgc acggcgtgga gatttatcgc ggccgaccca
     4021 ttctctacga tcttggcaac ttcatttaca acgtgccgcc cacgctcacc tacatcgacg
     4081 aacccatgaa ctgggagagt gcggtcgcct acgtgcaatt tcagaggaag aaactacaat
     4141 cgatttcgtt ccgcccaatt gtgctgaaca acgcgggcca gggccagccc gatattcaca
     4201 acgaatacgc caacaaccaa ttccttgaca cgcgcggctt gccctcgcca gccacgggtg
     4261 ccagagctgg ctacatcctc cagcgcctcg ccgactcctc aaagcccttt ggcacaacaa
     4321 ttgaggtaaa gggcaatatc gcggaaatca tactgaaacc aggaggttaa cctgaacttc
     4381 ttttcgaaat tcggcccttc atcgtttaca attgagttcg ttgcatcggc gttcctgccg
     4441 agaaggaggt gatccctgag gtacacggat aattagtgtc aactagaggg gcgcttgtga
     4501 gtttggccag acgcctctct tttcatttat tcccacatcg cgaaagaccg ccgcgatagc
     4561 aacagcattt ggcgcaccga agctaattgc aaaccgccgc ccgcttaagt ccgaggagcg
     4621 cgctcggaac agatcacaac cataaggaaa acacatgagc aaaacgattc aggtcgagat
     4681 cgaatatcac gatcgtaaca gcgggccgac tgtcgcaggt aaagcgcgcg tagaggcagg
     4741 cagcctgccc gtgggcattg gaaaggcgct tcgcgaagta tggaaaacga tggaccgcaa
     4801 agtccgcttc gacgccgcaa aaaatggcct gacgattaag gcccgagtcc tcgacgcttc
     4861 ggccacaatt gaaacgccaa gtgacacaaa gcaggttggc gagcaataag caaggggcgc
     4921 ggtcaaagta acgtccagcc aggccattct gattcttcca gtgtattccg ga
//
LOCUS       DATNDT010000177         3839 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1572637, whole genome shotgun sequence.
ACCESSION   DATNDT010000177 DATNDT010000000
VERSION     DATNDT010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1572637"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..768
                     /locus_tag="VN788_05780"
     CDS             244..768
                     /locus_tag="VN788_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXU20075.1"
                     /translation="MDFKLGEKVVYPNHGVGVIEQISFGYLNGRSERYYMLKIVSSGL
                     KVMVPQTNIMSVGLRPVIRSNEAGDVITYLEKGRSASHHDWKHRFKENSERMRTGSLM
                     EVAAVLKGLVSLSRTKPLSFREKKMLERAKHLLVTELATVRNTTEQTMETNLVRALAK
                     ARLQMPEALEKLEN"
     gene            complement(877..1581)
                     /locus_tag="VN788_05785"
     CDS             complement(877..1581)
                     /locus_tag="VN788_05785"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU20076.1"
                     /translation="MKKGLLFAVGVLLSAVPAMGFCSVPQPRLVCAEYFASEVVVEAT
                     LVNIRTISDKDDPEGISAFVYSLRAGQVFRGNIAGILQVYEGNDSGRATFDWKIGQTY
                     LLFLSHSASEKAWELDGCGNSGPVNRARAVLEEIATIQKRHGGDGHIYGVVSQQALSV
                     PIPQVQVEARGTDGVYKATTNAKGEFEIKVPPGVYAVRATKSGFRFGDADISYANSLN
                     LRVEPGGCVQLQLAAR"
     gene            complement(1578..3161)
                     /locus_tag="VN788_05790"
     CDS             complement(1578..3161)
                     /locus_tag="VN788_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXU20077.1"
                     /translation="MNFDPVRLEIFKNLFHSVAEEMGGVLRRSAFSPNIKERRDYSCA
                     VFDRAGDVLAMGDHMPVHLGSMPASVEAAIRAIDLKQGDVALLNDPYAGGTHLPDLTM
                     VMPAYLPREKTPMFYVANRAHHADVGGAQAGSMGLSREIFQEGIRIPPVKIFENGRIV
                     QDVLRLLLANVRTPEEREGDLTAQLAACRVGERRLIEITRKYGKQETGAYGRHLLEYS
                     AKMMRATLRQVPNGTYRGEDFLDNDGVTSDPIAIRVAIHIRGERAEVDFIGSSPECAG
                     SVNAVKAIADSAVFYVFRCLVDDQVPATSGLLRPIKIIAPEGTIVNARPPAAVAGGNV
                     EASQRIVDTLLRALARAIPKRIPAASQGTMNNLTFGGRDTRPGRGNAPFAYYETIAGG
                     MGARPGLDGESGIHTHMTNSLNTPVEVFEHAYPVRVKRYGIRYGSGGAGKFRGGDGII
                     REVELLVPMQVGMLSDRRKFGPYGLAGGKPGATGKNEVVIAGQRKMVPAKGSFYAPAG
                     AVVRIESPGGGGWGREKGR"
     gene            complement(3158..>3839)
                     /locus_tag="VN788_05795"
     CDS             complement(3158..>3839)
                     /locus_tag="VN788_05795"
                     /inference="COORDINATES: protein motif:HMM:NF039581.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXU20078.1"
                     /translation="AGGLHACGLAAALEMPRVLIPKFPGGLSALGILRADVVKDFSMT
                     ILLAVQSGREANIALRREFAQIEKKARAAMRQEGFESRAMKIERILDMRYVGQAYELS
                     VPATADFVRSFHRAHEKRYGYADAKRSVEIVNVRARATGLTPKPALPRLPRGGAGVST
                     AVMQERSVFFSGRATKTKVYDRAKLQAGSRFRGPAIVAEYSATTVVLPGWNVRVDVHG
                     NLLMERGR"
BASE COUNT          785 a         1211 c         1030 g          813 t
ORIGIN      
        1 aaagtcgaga tcactcccgc caatttgccg tctcgcagaa cggccaaact tcccaatcga
       61 gaagaggtgt cgacggccag aagcagcata aacggccatt ttacaagatt tctggcttga
      121 cttttggact aaataagcgc ctaaaagaga caaaaatata agggttttcc cttagattct
      181 cttgaccttc accttacgca ggcctaagct tttggtgtag cttctgggga gggtacatcc
      241 tgtatggatt tcaaattggg cgagaaggtt gtttatccaa accacggtgt aggcgttatc
      301 gaacaaatca gcttcggtta cctgaatggc cgctccgaac gttactacat gctgaaaatc
      361 gtttccagcg ggctaaaagt catggttcca caaaccaaca tcatgagcgt cggtctgcgt
      421 cccgtgatcc gctcgaacga ggcgggtgac gtcattacct atctcgaaaa gggccgctcc
      481 gcctctcatc acgactggaa gcaccgcttc aaagagaact ccgagcgtat gcgcactggc
      541 tcgctgatgg aagtcgccgc ggtcctgaag ggactggttt cactcagccg gactaagccg
      601 ctttccttcc gcgagaagaa aatgctcgaa cgcgccaagc atttgctggt gaccgagctc
      661 gctaccgtcc ggaataccac cgagcagact atggaaacga acctggttcg tgcactggcc
      721 aaggcccgtt tgcagatgcc ggaagcgctc gaaaagctcg agaactaact actactgcat
      781 gcgggggctc cagcgccccc gcgcaattct ccctcgccat ttcgcgtttg acgtcttccc
      841 gaaggacgcg ttcccttcac agggcccttg catgcactac ctagcggcga gttgaagctg
      901 cacgcatcct ccgggttcga cccgcaagtt tagcgaattg gcgtagctta tatccgcatc
      961 cccgaaacga aacccgctct tcgtagcgcg gacagcataa acaccgggcg gtacttttat
     1021 ctcgaattcc ccctttgcat tcgtggtcgc cttgtaaact ccatcggtac ccctcgcctc
     1081 gacctgcact tgaggaatgg gcaccgacag cgcctgttgg ctgacgacgc cgtatatatg
     1141 accgtcccct ccgtgacgtt tttggatggt tgctatttcc tccaacacag ctcttgcacg
     1201 gttgaccggg ccggagttgc cacatccatc gagctcccac gccttttcgg aagccgagtg
     1261 ggagaggaat agcaagtacg tctgcccaat cttccaatcg aacgtcgcgc gaccgctgtc
     1321 gttgccctca tagacttgaa ggattccggc aatattgccg cgaaacactt gccctgcacg
     1381 caggctgtag acgaatgccg agataccttc tggatcatcc ttgtcgctaa tggttctgat
     1441 gttcacgagc gttgcctcaa ctacgacctc gctcgcgaag tactccgcgc aaacaagccg
     1501 tggctgtgga acggagcaga accccatcgc aggaaccgcg ctgagcagca cgccaactgc
     1561 aaagagaagc ccttttttca tcgccctttc tcccttcccc agccgccgcc gccggggctt
     1621 tcgattctga ctacggctcc cgcaggagca taaaaactgc cctttgctgg aaccatcttc
     1681 cgctggcctg ctatcaccac ttcgtttttc ccggtagcgc ccggcttgcc tcccgcaagg
     1741 ccgtaggggc cgaacttgcg gcgatccgac agcattccca cctgcatcgg cacgagtaat
     1801 tcgacctcgc ggatgattcc gtcgccaccg cgaaacttcc ccgctccgcc agaaccgtac
     1861 cgtatgccat aacgtttgac gcgtacggga tacgcatgct cgaagacttc caccggcgta
     1921 ttcagcgaat tggtcatgtg cgtgtgaata ccgctctcgc cgtcgagccc cgggcgtgcc
     1981 cccatgcctc cggcgattgt ctcgtaatag gcaaatggcg cattgcctcg gccgggccgc
     2041 gtgtcgcgac cgccgaaagt caaattattc atggtgccct ggctcgctgc ggggattctc
     2101 ttgggaatgg ctcgcgcgag cgcgcgcaac agcgtgtcga caatcctctg cgacgcttcc
     2161 acgttccctc ccgcgacggc tgcgggcggc cgggcattca cgatggtacc ctcgggcgcg
     2221 atgatcttta tcggacgcag aaggcctgac gtcgcgggga cttggtcatc caccaaacac
     2281 cgaaaaacgt aaaacacggc cgagtccgcg atggctttga cagcgttgac gcttccggcg
     2341 cactccggcg acgaaccgat gaaatccact tccgcgcgtt cgccgcgaat atgaatggcg
     2401 acacgaattg ctattgggtc gctggtgacg ccgtcattgt cgagaaaatc ctctccgcga
     2461 taggtaccat tgggaacttg gcgcagtgtc gcgcgcatca ttttcgcgga atattccagg
     2521 aggtggcgtc cgtaagcgcc agtttcctgc ttcccatact tccgcgtaat ctcgatgagc
     2581 cgcctttcgc cgacgcgaca tgccgcgagt tgcgccgtca aatcaccctc gcgctcttca
     2641 ggcgtccgca cattcgccag cagcagacgc aacacgtctt gaacgatgcg gccgttttcg
     2701 aaaatcttca ccggaggaat gcgaatgccc tcctggaaga tttcgcgcga cagtcccatc
     2761 gagcctgcct gcgcgccgcc aacgtccgcg tggtgcgcgc gattcgcgac gtagaacatc
     2821 ggcgtctttt cgcgcggcaa gtacgcgggc atcaccatgg tcaagtccgg cagatgcgtt
     2881 ccgcccgcgt atggatcgtt caacagcgcc acgtcgccct gcttcaaatc gatggcgcga
     2941 attgcggcct ccactgacgc aggcatcgac ccgagatgca ccggcatgtg atcgcccatc
     3001 gcgagcacgt ctcccgcgcg atcgaagacg gcgcaggagt agtcgcggcg ctctttgata
     3061 tttggcgaga aggccgaccg gcgcaggact ccgcccattt cctcagcgac ggaatgaaac
     3121 aggtttttga aaatttccag gcgcacggga tcgaagttca tcggccgcgc tccatcagca
     3181 gattgccgtg cacatcgacg cgaacgttcc agccgggcag cacgacggtg gtcgcgctgt
     3241 actcggctac aatcgctgga ccgcggaagc ggcttcccgc ttgcagtttc gccctgtcgt
     3301 agacttttgt tttcgttgcc cggccgctga aaaatacgct gcgttcttgc atcacagcag
     3361 tcgaaacacc ggctcctccg cgtggaagtc ttggcaacgc tggcttggga gtcaagccgg
     3421 tcgctctcgc acgcacgttc acaatttcga cggagcgctt cgcgtcagcg tatccgtatc
     3481 gcttctcgtg cgcgcgatgg aagctgcgca caaaatccgc ggtcgccgga acgctcaact
     3541 catacgcttg accgacgtag cgcatatcga ggatgcgttc gatcttcatt gcacgactct
     3601 caaaaccctc ctggcgcatt gccgcccgcg cttttttctc gatttgagcg aactcgcggc
     3661 gcagcgcgat atttgcctcc cgcccggatt gcacagccag cagaattgtc attgagaaat
     3721 ccttaactac gtccgcgcgc aaaatcccca gcgcggaaag tccgcccggg aatttcggta
     3781 tgagcacgcg gggcatttcg agcgccgcgg caagcccgca tgcgtgcaag ccgcccgcg
//
LOCUS       DATNDT010000178         4132 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3126647, whole genome shotgun sequence.
ACCESSION   DATNDT010000178 DATNDT010000000
VERSION     DATNDT010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3126647"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            420..1850
                     /locus_tag="VN788_05800"
     CDS             420..1850
                     /locus_tag="VN788_05800"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HXU20079.1"
                     /translation="MSNELRSFLEIPYGELEELNLKAKEQRQSRVPMEKLQEERVKYL
                     TDEKRIKAVTVLFSDLEGRLHMLDYDKKFLVKSWDNLTFDGSSIRGFTAQRESDLRLG
                     IDWSAFYWAPADFFGSGKVMVFGDVIDKQGGPYAADMRAMLKSFSDSMYARDGYTLNA
                     ANEIEGFLFAGTDAERRYYETGKFEYVNTGGYYHSLPGDPLRTFIDTVAEVQRAMGFQ
                     NEKDHPEVAPSQFEINYGYGEVVAAADQIQLYKLICREVATRSGMTASFLPKPVVGVN
                     GSGMHTNVSISKNGTNLFWDPKGQEKLSSCGWEFIDRILTHGNDICLALNPSVNAYRR
                     LDPHFEAPNQIKASAVDRGSMIRVPIGNSKSMRVEVRSVAPDANPYLVMLSVFRTGIE
                     GDTADLENLRQAERYLPDNIYTAIDDFRAAEWTTKLLGEDVKGRYADLKQASADRCAR
                     LLGTVVKGPEVQFHHEVYNQYLWNQF"
     gene            complement(1948..2664)
                     /gene="bshB1"
                     /locus_tag="VN788_05805"
     CDS             complement(1948..2664)
                     /gene="bshB1"
                     /locus_tag="VN788_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HXU20080.1"
                     /translation="MKLDLLAIAPHPDDVELTCGGTMLKMAQAGYRTGILDLTRGETG
                     TRGTPETRLKEAARAGKILGAKIRRNLGLPDAHLKVCDEQKEAIAAVIRELEPHTVIL
                     PYWQGRHPDHYTAAVLGWEACFVAGLKNYALAGEAFRPFKIIYAAAYADVRPTFAVDI
                     TAQYDRRRQAILAYASQFRPPKRDRRTKVSLPLDELEEYMGGIAKHYGRMIGVKYAEG
                     FVVKEIMRAEDVVKLPVRSM"
     gene            2782..3513
                     /locus_tag="VN788_05810"
     CDS             2782..3513
                     /locus_tag="VN788_05810"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU20081.1"
                     /translation="MAGKPLEGQVALITGAAKRIGRSIALRLAADGSAIAVNYKTSRG
                     EAESLVRELHDAGAKAAAIPADVSKRAEVEKMFADVEREFGRLDILVNNAGMFFSAKF
                     ESLNDEQWDRIMDTNLKSQFLCAQVAAPMMKRQGRGRIINLSSLGGLLAWPSYTHYCV
                     SKAGSIMLTRCLARALAPEILVNSVAPGTIQFPGEPPDEEYIRRVPLHRTGTGDDIAG
                     AVAYLATADFVTGQVIVVDGGRALV"
     gene            3560..3915
                     /locus_tag="VN788_05815"
                     /pseudo
     CDS             3560..3915
                     /locus_tag="VN788_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013111523.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
BASE COUNT          885 a         1054 c         1282 g          911 t
ORIGIN      
        1 tgatcgggta gctgacgtgt tgccatacgc cgggcacatc gataaaacgc aggtcaagta
       61 ttttagcgtt gtttttcgac gcgaattcaa gaacgccttt agaatccatc tgccctccaa
      121 gtgcactgcc gcgaggtcaa gtttgcgcgc agaagttaac ctagggcttg ggtggcgtca
      181 agcaaagggt tggggtgggc tcaatgcttt ttttcatggg atggataggt ttttgttatg
      241 gaattgcctg agcgtcagcg atgcgaggcg ccgcatgcga agacaaatcc ggtcgatcac
      301 ttctttcgat tggaagcacg tgccgatccg cgatatcgta tcgttgcaat tctggacttc
      361 tgtcctggtt gcgcgatctt tataaccgag aagttcattt caaaagatga aggagataca
      421 tgtccaacga acttcgcagc tttctcgaga ttccatacgg tgaactggaa gaactgaatt
      481 tgaaagccaa ggagcaacgg caaagccgcg ttcccatgga gaagctccag gaagaaagag
      541 tcaagtatct gacggatgaa aagcgaatca aagcggttac ggtgcttttc agcgatctgg
      601 aaggccgcct gcacatgctg gactacgaca agaaatttct ggtcaagagc tgggacaacc
      661 tgacatttga cggctcgtcg attcgaggat tcacggcaca gcgcgagagc gatttgcggc
      721 taggaatcga ttggagcgcg ttttactggg ctccggcgga ctttttcggc tccgggaagg
      781 taatggtttt cggcgatgtg atcgacaagc agggcgggcc ctatgccgcc gatatgcgcg
      841 ccatgctgaa aagtttttcg gatagcatgt acgccaggga cggatacacg ttgaacgcgg
      901 ccaacgaaat cgaagggttt ctctttgcgg gcacggacgc agagcggcgc tattacgaaa
      961 cgggcaaatt cgaatacgtt aataccggcg gttattacca ttcgcttccg ggcgatcctc
     1021 tgcgcacgtt catcgacaca gtggccgaag tgcagcgcgc catgggtttc caaaacgaga
     1081 aggatcatcc tgaggtggcg ccatcgcaat tcgaaatcaa ctacggttac ggggaagtgg
     1141 tcgcggcggc ggatcagatc caactctaca aactgatctg ccgcgaagta gcaacgcgat
     1201 ccggtatgac agcgtcgttc ttgccgaagc cggtggtggg cgtgaacggt agcgggatgc
     1261 acacgaatgt ttcgattagc aagaatggga cgaatctctt ctgggatccg aaggggcagg
     1321 aaaagctttc gagctgcggc tgggagttca tcgaccggat cctcacacac ggaaacgaca
     1381 tttgcctggc gctgaatccg agcgtgaacg cgtatcgccg cctcgatccg cacttcgagg
     1441 cgccgaatca gatcaaagcg tcggcggtcg atcgcggctc gatgattcgc gtgccgatcg
     1501 gcaactcgaa gagtatgcgt gtggaggttc gctccgtggc tcccgatgcg aatccgtact
     1561 tggtgatgtt aagcgtgttt agaacgggta ttgaaggcga tacggccgac ctggaaaatc
     1621 tgcggcaggc cgagcggtat ctgccggaca acatctacac ggcgatcgat gatttccggg
     1681 cagccgagtg gacaacaaag ttgcttggcg aggacgtgaa aggccgctat gccgacctca
     1741 agcaggcgtc cgccgatcgt tgcgcgcgct tgctgggcac ggtggtaaaa ggtccggagg
     1801 tgcaattcca ccacgaggtt tacaaccaat atttgtggaa ccagttttaa gtaattggcg
     1861 acgaaagtgc gaagtgggaa ggccctggcg cgcgacctgc gctggggcat tccagttttc
     1921 gtccgagtga gcgaattctt tttcgcttta catggatcga accggcagct tcacaacgtc
     1981 ttctgcgcgc ataatttcct tcacgacgaa gccttccgcg tatttcaccc cgatcattcg
     2041 gccataatgc ttcgcgatgc cgcccatgta ctcttcgagt tcatccaacg gaagtgaaac
     2101 ttttgtgcgg cggtcgcgct ttggcgggcg aaattgtgat gcatacgcga gaatcgcttg
     2161 gcggcggcga tcgtattgcg cggtgatgtc gaccgcaaag gtcgggcgaa cgtccgcgta
     2221 cgcggcggcg taaatgatct tgaaggggcg gaacgcctcg ccggccaatg cataattttt
     2281 taggccggca acgaaacaag cctcccatcc gagaactgca gcggtgtaat gatctgggtg
     2341 gcgtccttgc cagtacggaa ggattacggt gtgcggttcg agctcgcgaa tcacagcagc
     2401 gatggcttcc ttttgctcgt cacacacttt cagatgggcg tcgggcaacc cgagattccg
     2461 gcggattttc gcgccgagta ttttccccgc gcgcgctgct tccttcagtc tcgtctccgg
     2521 agtgccgcgc gtgcctgttt cgccgcgcgt aagatcgagg atacctgtgc gatagccagc
     2581 ttgcgccatt ttgagcatgg tgccgccgca ggtgagttcc acgtcgtccg gatgcggcgc
     2641 aattgccagc aagtcgagtt tcattcgtcc tccggttcgc gacgcacgat tttacatgaa
     2701 agcgacgcga gaaaaggtga tcggagactc cttcggaagc ttgatagtgt taatgtgtcc
     2761 gatgcattca ggagttacgc aatggcgggg aaacccctgg agggtcaggt tgcgctgatt
     2821 accggcgcgg cgaagcgcat cggacggagc atcgcgctgc gtttggccgc ggatgggtcc
     2881 gcgatcgcgg tgaattacaa aacgtcgcgc ggcgaagccg agtcgctggt tcgcgaactt
     2941 catgatgctg gagcgaaggc cgcggcgatt ccggcggatg tttccaagcg cgcggaagtg
     3001 gagaaaatgt ttgccgacgt agaacgcgaa ttcgggcggc tggacattct cgtcaacaac
     3061 gccgggatgt tcttctccgc gaaattcgag agcctcaacg acgagcagtg ggatcgcatc
     3121 atggatacga acctgaaatc gcagttcctc tgcgctcagg tggccgcgcc gatgatgaag
     3181 cgtcaggggc gcgggcggat catcaatctc tcgtcgctcg gcggcttact cgcgtggccg
     3241 tcctacacgc actattgcgt gtcaaaagcg ggctcgatca tgctgacgag gtgcttggcc
     3301 cgggcgttgg cccctgaaat tctggtgaac agcgtagctc ccggtacgat ccagtttccc
     3361 ggcgagccgc cggatgaaga atatattcgc cgcgttccgc tgcatcgcac cggcacggga
     3421 gacgatattg ctggagccgt cgcgtatctt gcgaccgccg atttcgttac cggacaagtg
     3481 atcgtcgtcg atggcggcag agcgctcgtg tgatccggtg cggcagtcgt gatgtgggag
     3541 tttgaatcgc gaggtgatca tgccgagttt gattgagaag ccaacccaaa tcgtggcggc
     3601 gggaacgaag ccgaagctca tcgaggaatt tgtgggtcac gtaaactcga agactgcggg
     3661 cgtgagcgtg gcgcgcatgc gaagtcccgc agggtggtcg gagccgggtc aaacgccgga
     3721 atttgacgag tacagcatcg tactgagcgg aacgcttcgc gttgcgcaca aatcaggttc
     3781 cttagaggtt cgcgctggcc aagcagtgat cgcgcagggc ggggaatggg tgcagtattc
     3841 gacgccggag gacgcggagt acatttcggt gtgtgtctgc cggcgttttc tccgaccaac
     3901 gtgcaccgcg attgagcgtt tttttatcga gtgcctctcg caaacacgca aaatcctgtt
     3961 tgcaaaagtt gagttctcga atattctagt tagttcgggg gtgcacaact agggtgtcag
     4021 agtcttcaac cgcaggggga ctgactcgtt ttgggagcac gcagcggcga gacgcgtggt
     4081 ggatcgagaa cctaccgttc atcatcgtcc tcggcgcttt cactgtttac ac
//
LOCUS       DATNDT010000179        11711 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3007629, whole genome shotgun sequence.
ACCESSION   DATNDT010000179 DATNDT010000000
VERSION     DATNDT010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3007629"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            75..1499
                     /locus_tag="VN788_05820"
     CDS             75..1499
                     /locus_tag="VN788_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HXU20082.1"
                     /translation="MKSSYNGVPVPAQGKAIGYSGGEFQIPDQPIIPFIEGDGTGRDI
                     WKASRRVFDAAVEKVYGGKKKIAWLEVYAGEKSFNQFKDWLPQDTVDAIRDFRVGIKG
                     PLTTPVGGGIRSLNVTLRQLLDLYACVRPVRYIEGVPSPVRNPERMDVVIFRENTEDV
                     YAGIEWKSGTPEVKKLIDFLNNEMLKDGKKKVREDSGVGIKPISPSGTKRLVRRAIQH
                     AIEHRRTVVTLVHKGNIMKFTEGAFRDWGYELATEEFRGQIVTERESWILGNKDRDAN
                     LTIEQNAAMIEPGLDQATDDFKKKICAEVKQTLDAIGATHGRGQWKKKILVNDRIADS
                     VFQQVLTRADEYQVFATPNLNGDYISDACAAQIGGLGMAPGANIGDGYGCFEATHGTA
                     PKYADKDVINPSSVMLSGAMMFDFMGWKEVARLIEDGIGRTIQQKRVTYDLERLMAGA
                     TKVGTAEFASAIIDNMHAPATVGR"
     gene            1560..2498
                     /gene="mdh"
                     /locus_tag="VN788_05825"
     CDS             1560..2498
                     /gene="mdh"
                     /locus_tag="VN788_05825"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HXU20083.1"
                     /translation="MRKKVTVVGGGFVGSTTAQRIHDAGLADVVLTDILEGVPAGKAL
                     DMAESAPILGADVHATGISTASGDYKETANSDIIVITAGFPRKPGMSRDDLLKANYDV
                     IKAVVEAVVKLSPNAILIVVTNPLDAMAQAAFKVSGFPKSRVLGMAGVLDSARMSTFV
                     AAELDVSVENVHSFVLGGHGDDMVPLARYSTVAGIPLPELIAKEKLEGIITRTRKGGA
                     EIVNLLKTGSAYYAPSAAVAEMVDAILKDKKKILPCAVYLEGEYGIRGLFVGVPVKLG
                     AKGVEQIIEIKLTPEEKSALDKSSASVRELVNVLGL"
     gene            complement(2511..4148)
                     /locus_tag="VN788_05830"
     CDS             complement(2511..4148)
                     /locus_tag="VN788_05830"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXU20084.1"
                     /translation="MKIALAQFNPTVGDFEGNRARILDLARDAKSGGAEIAVFSELCL
                     CGYPPQDLIERPAFAERNQTELARLARDISLPSLIGFVGKAQDDTGKPVANSAALVGC
                     GKILFEQRKMLLPTYDVFDESRYFQPAHTQFAFPFNTETLGITICEDSWNDKSFWPER
                     LYDRDPVSELVGKGSTLILNISSSPYRLGIRGLRQDMLRAIAIDRRVPVVYVNQVGGN
                     DSLIFDGSSVAFMPDGRVAALAKSFDEDLVFFDSVTGGGDIRAPMEDELEAAYRALVI
                     GTRDYVRKCGFRKVVIGLSGGIDSALVAVIAADALGPVNVTGVAMPGPYSAPSSLHDA
                     QRLAENLGIHLIAMPILDTFASYRKTLGTTFAGLSDDVCEENIQARIRGNLLMALSNK
                     FGSLVLSTGNKSELAVGYCTLYGDMAGGLAVISDVPKTMVYDLARFVNRSGVRIPPEI
                     IGKVPTAELRPNQTDQDTLLPYEDLDRIVKAYVEDLQSPEKIAEECLLPLDVVRAVAV
                     QVDKSEFKRKQSPPGLKVTSKAFSVGRRFPLAQKYSV"
     gene            complement(4320..4646)
                     /gene="erpA"
                     /locus_tag="VN788_05835"
     CDS             complement(4320..4646)
                     /gene="erpA"
                     /locus_tag="VN788_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HXU20085.1"
                     /translation="MVQLTPVAVNKVKEILSQQTPEPTGLRVAVVGGGCSGFSYKMAF
                     ENQVNEASDNVYEFDGLKVLVDQMSEMYLDGISIDYIETLEGAGFKFNNPNVKSTCGC
                     GSSFTV"
     gene            4836..5840
                     /locus_tag="VN788_05840"
     CDS             4836..5840
                     /locus_tag="VN788_05840"
                     /EC_number="1.1.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005962 - mitochondrial isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_component: GO:0045243 - cytosolic isocitrate
                     dehydrogenase complex (NAD+) [Evidence IEA];
                     GO_function: GO:0004449 - isocitrate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006102 - isocitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NAD(+))"
                     /protein_id="HXU20086.1"
                     /translation="MKHAITLIPGDGIGPEVSAAVQTILAAAGVQIEWEQASAPSDIH
                     QRGSGRMQAELVESIRKNGVALKGPLATGIATGPPSVNVGLRKALDLYANVRPVKNLE
                     GVKSRFQDIDLVIIRENTEDLYAGLEHEVVPGVVESLKIITEKASTRVARFAFEYARK
                     HGRKKIHGMHKANIMKMSDGLFLASVRKVAAEYSDIEYKEMIIDNACMQIILDPYQFD
                     MLLLTNLYGDIMSDLAAGLVGGLGVVPSGNIGASAAIFEAVHGTAPDIAGKGVANPTA
                     LLMSAIMMLDYLDEVKAARRIEDALHKVYREGKYLTRDVGGTTTTLQFTEAVIGGLDP
                     "
     gene            complement(5865..6260)
                     /locus_tag="VN788_05845"
     CDS             complement(5865..6260)
                     /locus_tag="VN788_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20087.1"
                     /translation="MLKLPSQLRAALVLVAMVSACVPFLAAGEKQEVFKGEISDSQCA
                     FNVHSTTHSHDEMLKTRTMGNNPADCVHMCVDNLGRVYVLRTKDKVYKLDKQNLAGKY
                     PAQAVKVTGVVNPKGNTIAVRSIVALDAE"
     gene            complement(6451..6831)
                     /locus_tag="VN788_05850"
     CDS             complement(6451..6831)
                     /locus_tag="VN788_05850"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HXU20088.1"
                     /translation="MVERCSSIIVLALLIVSAAIGVSAGSNDQVFKGEIGDSQCALNV
                     HSLSRSHREMLERKSIGKTSADCARYCVKNLGGVFVLQVKDKVYKLDNQELADKNAGL
                     KVEVVGVLDPKSNVIAVTSIEPLR"
     gene            complement(6887..7330)
                     /locus_tag="VN788_05855"
     CDS             complement(6887..7330)
                     /locus_tag="VN788_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20089.1"
                     /translation="MDVDKNPRVSSLRLICLVAVAFLFVLSPHFVWSQSPQVIEVLAD
                     HDSRFKMAGMKDPVITVHAGAPVTLRITAVKAKDHNRDGSIHGFSLLRANDRSPVPGW
                     DLLLKPGRQEFNLTAPAEPGEYVVVCTVVCSPDHDGMHMKFIVLP"
     gene            complement(7342..7692)
                     /locus_tag="VN788_05860"
     CDS             complement(7342..7692)
                     /locus_tag="VN788_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20090.1"
                     /translation="MTAKWLAPTVVMLGVLTLCSSLASAQEAKKLAASAPDSVPQVAG
                     SQPNAASSSASSQEDARWRFEGQKRFATNCGRCHQAPHKYPPRVMATVIRHMRVRATI
                     TDDDMRYILRYITQ"
     gene            complement(7689..8771)
                     /locus_tag="VN788_05865"
     CDS             complement(7689..8771)
                     /locus_tag="VN788_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20091.1"
                     /translation="MLFARFNSILSTGLRILFVAAALFGVAVGQANGQLEDSHAIKPV
                     PVFTMGAGFISSFEGGTPHLGPLVSPIFLIPIGQNWLIESRGTFEADLAPPPGSNDFK
                     GDVEKEVDYLQLDYIANRYTTVSIGRFLTPFGIYNERLYPVWIRDLQSDPLILPIGVG
                     PSNASTGAMLRGGFNITPKIELNYAAYFSAESSANRFESDRAIGERVGIFLPGPRLEV
                     GGSFEHLLQDNRSNAFGFHFAWQPRPLPLDIRGEYARSQNGSGYWIESAYRLSQFPVW
                     NDEMRHVQAVARMQQFYVGSSPGESVLPVNTNMFEFGLNYYFQDNFRFVSSYGRQFAP
                     LGGNENVWTLGLTYRLVIPLGDGEGE"
     gene            9044..9727
                     /locus_tag="VN788_05870"
     CDS             9044..9727
                     /locus_tag="VN788_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20092.1"
                     /translation="MPSPAKFKAAYIYRIFTWSSLAVLVLALALIFKKPHAPSVPSDP
                     AAAARVEQKFDEADEAEATGKPVDVDLDRTELNSYLHQNLQLDGSQPDVVAAGPATSA
                     SASDTQQPDPTAAIAGNGVTLEQAQSSVKDVNVDLDGDLVKAYVTFDFHGKDLALELD
                     GHLGTRDGYLKFDPVAGKLGSLPLPQSTLQAAVNKMMASPENREKLRLPPDISNIEVS
                     NGQAVISYK"
     gene            complement(9729..10172)
                     /locus_tag="VN788_05875"
     CDS             complement(9729..10172)
                     /locus_tag="VN788_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HXU20093.1"
                     /translation="MRSPFLAMLPAANAGLNAACTVFLLCGYVFIRNHKVRWHRACML
                     SAFGCSVVFLVFYLYFHMHAGVIRFSGQGWIRPVYFTILVSHTIFAAVIVPLVLITLA
                     RALRERFDRHRAIARWTLPIWLYVSITGVVVYWLLYVAYTPIWPA"
     gene            complement(10246..11142)
                     /gene="cyoE"
                     /locus_tag="VN788_05880"
     CDS             complement(10246..11142)
                     /gene="cyoE"
                     /locus_tag="VN788_05880"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HXU20094.1"
                     /translation="MGNTRADTLSTFDKPWAYVVLTKPDVTFLVVITTVAGFYLGSTG
                     PLDWVLMLHTLGGTLLVAGGTAALNQYVERKMDAVMRRTASRPLPSGLLQPKDVLIFG
                     LGTIVAGAAWLALLVNGLAALVALATCVLYLGLYTPLKTRTTLSTAVGAIPGALPPLI
                     GWAAARGSLSLGGWVLFAILFFWQFPHFMAIAWMYREDYARAGIRMLPVVDRKGDSTF
                     RQIIWTSAVLVWVSALPAVLGMAGKYYFFGALILGMILLQVGLWANRTRTNARAKWLM
                     HATVAHIPLLLAWLIVNRLITR"
     gene            complement(11150..>11711)
                     /locus_tag="VN788_05885"
     CDS             complement(11150..>11711)
                     /locus_tag="VN788_05885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20095.1"
                     /translation="AQIFFITIVGLTFYLSGWWQSDHPELDDRGSPRLRTLAAWTTAA
                     ILVQLVLGAAFRHGAFGIDPHLVGAGVVTVMVVWTGVVAKKRFRQERPLRRATILLHS
                     FFGIQILLGFAAWWAVAAARDAVQPTFTYVSLTVAHVLGGALTLAASVIVTLTSVRLI
                     RRATPMAVESRVISSGGEKSPERAGV"
BASE COUNT         2698 a         3181 c         3438 g         2394 t
ORIGIN      
        1 acagcatgcg cggattgtgc taaggtattt ggttccgtcc tcgcatcgct agacagcata
       61 ggagatggca atcgatgaaa agttcgtaca acggggtacc cgttccagcg cagggcaagg
      121 ctatcggata cagcgggggt gaatttcaaa ttcccgacca accgatcatt ccatttatcg
      181 agggcgatgg cacggggcgc gatatctgga aggcgtcgcg gagagttttc gacgcggctg
      241 tggagaaggt gtacggcggg aagaaaaaga ttgcgtggct ggaggtgtac gccggagaga
      301 aatcgttcaa tcaatttaag gactggctgc ctcaagatac tgtggacgcc attcgcgatt
      361 ttcgtgtggg aatcaaaggc ccgctgacaa ctcctgtggg cggcggaatt cgttcgctca
      421 acgtgacctt gcggcaactg ctcgatctct atgcgtgcgt gcgcccagtg cgctatatcg
      481 agggagtgcc ttcgccggtg cggaatcccg agcggatgga tgtggtgatc ttccgcgaaa
      541 ataccgagga cgtttacgct ggaatcgaat ggaaatccgg aacgcccgag gtgaagaagc
      601 tgattgattt tctgaacaac gagatgctga aggatggcaa gaaaaaggtg cgcgaggatt
      661 cgggcgtggg cattaagccg atttctccga gtggcaccaa gcgcctggtc cgccgcgcaa
      721 ttcagcatgc catcgaacat cggcggacgg tggtgacgct ggtccacaag ggcaacatca
      781 tgaagttcac cgagggcgct tttcgcgatt ggggctacga gctggccacc gaggaatttc
      841 gaggtcagat tgtgaccgag cgcgagagct ggattttggg aaataaggat cgcgacgcga
      901 atcttactat cgaacaaaat gcggcaatga tcgagcccgg gctcgatcag gcgaccgacg
      961 atttcaagaa aaagatttgc gcggaagtta agcagacact cgacgccatt ggcgctacgc
     1021 acggccgggg acagtggaag aaaaagattc tggtgaacga taggatcgcc gattccgtgt
     1081 tccagcaagt gcttacgcga gcggacgaat atcaggtgtt cgccacgccg aatctgaatg
     1141 gcgattatat ttccgatgct tgcgcggcgc agatcggcgg actgggcatg gctccgggcg
     1201 caaatatcgg cgatggctat ggatgttttg aagccacgca cggaacggcg ccgaaatacg
     1261 cggataagga tgtgatcaat cccagctcgg tgatgctttc gggcgcgatg atgttcgatt
     1321 tcatgggctg gaaagaagtg gcgcggctga tcgaggacgg gatcgggcgc accattcagc
     1381 agaagcgcgt gacgtatgat ctcgagcgct tgatggctgg agcgaccaag gtgggaaccg
     1441 cggaattcgc gagcgcaatt atcgataaca tgcacgcccc cgccacggtg ggacgataaa
     1501 gcgggagttg ttcacgcgca gcaggccgaa tttatcctca ataatcagga gattggcgaa
     1561 tgcgtaagaa agtgacggtt gtgggcggag ggtttgttgg gtcgacaacg gcacagcgga
     1621 ttcatgatgc aggcttagcg gatgtagtgc tcacggatat tctcgaagga gtgccggctg
     1681 ggaaagcgct cgatatggct gagtcggcgc cgatccttgg agcggacgtt cacgctacgg
     1741 gcatttcgac ggcgagcggt gattacaagg agacggccaa tagcgacatc attgttatca
     1801 cggcgggatt tccgcgaaag ccgggaatga gccgggacga tctgttgaag gcgaactatg
     1861 acgtaattaa agcagtggtt gaagctgtgg tgaagctttc gccgaacgcg attttgatcg
     1921 tggtgacgaa tccgcttgac gcgatggcac aggccgcgtt taaggtaagc ggcttcccga
     1981 agagccgagt gctgggcatg gccggcgtct tggactcagc gcgcatgagc acgttcgtgg
     2041 cggcggagct ggacgtgtcg gtcgagaacg tgcattcgtt tgtgctcggc gggcatggag
     2101 acgatatggt gccgctggcg cggtattcga ctgttgcagg gattccgttg cccgaactta
     2161 tagctaaaga aaaacttgaa ggcattatta cgcgcacgcg gaagggtggc gcggaaattg
     2221 ttaacttgct gaagactggc tcggcttact acgcgccttc ggctgccgtc gcggaaatgg
     2281 tggatgcgat cctaaaagat aagaagaaga ttcttccgtg cgcggtgtat ttggagggcg
     2341 agtacggaat caggggcttg tttgtgggcg tgcccgtaaa gttgggcgca aagggcgtgg
     2401 agcagattat cgaaattaaa ctgacacctg aagaaaagag cgcgctcgat aagtcttctg
     2461 cttcggttcg ggaattggtg aatgttctcg gattgtagag tggaaatcgc ttagacggag
     2521 tatttctggg cgagtgggaa tcgccggcct acgctgaaag cttttgaagt gactttgagg
     2581 ccgggcggcg actgcttgcg cttgaattcg cttttgtcca cttgaaccgc gacggcgcga
     2641 actacgtcca gaggtagaag acactcctct gcaatttttt cgggactctg caaatcctca
     2701 acgtatgctt tcacaatgcg atcgaggtct tcgtagggca gcagggtatc ctgatcggtc
     2761 tgattggggc gtagctcggc tgtcggcacc ttgccaatga tctccggcgg gattcgcact
     2821 ccgctgcggt tcacaaacct cgccagatcg tagaccattg tttttggcac gtcggaaatt
     2881 acggcgagac caccggccat gtcgccatac agggtgcagt aacccactgc caactcagat
     2941 ttatttcctg tgctgaggac tagcgaacca aatttattgg aaagcgccat caggagattg
     3001 ccgcggatgc gggcctgtat gttctcttcg cagacgtcgt cggacaagcc cgcgaatgtc
     3061 gtcccaaggg ttttgcgata gctggcgaat gtgtcgagga tcggcatggc gatcagatgg
     3121 atgcccaggt tttccgcaag gcgttgtgcg tcatgcaagc tggaaggggc cgagtacggc
     3181 ccgggcatgg ccacgccggt gacgttcacg ggccctagcg catcggcggc gatgacggcg
     3241 acgagcgccg agtcaatgcc gccgctcagg ccgattacga ctttgcgaaa gccgcacttt
     3301 cggacgtaat ccctagttcc gattacgagc gcgcggtagg cggcctcaag ctcatcttcc
     3361 atgggcgcgc gaatgtcgcc cccgcctgtg acgctatcga aaaaaacgag atcctcgtcg
     3421 aatgatttgg cgagcgcagc gacgcgccca tcgggcatga aagcgacgct ggaaccatcg
     3481 aagatgaggc tgtcgttgcc gccgacctgg ttgacgtaga ctaccggaac gcggcggtcg
     3541 atggcgatgg cgcgaagcat gtcctgccgg aggccgcgta ttcccaggcg atagggcgac
     3601 gacgaaatat tgaggatcag cgtgcttccc ttcccgacga gttcggagac ggggtcgcgg
     3661 tcgtagaggc gctccggcca aaagctttta tcgttccagc tgtcctcaca aatggtgatc
     3721 cccagcgttt cagtattgaa tggaaatgcg aactgggtgt gggccggctg gaagtagcgg
     3781 ctctcatcga aaacatcgta ggtagggagc agcattttgc gctgttcgaa gagaattttc
     3841 ccgcagccga ctaacgcagc ggaattggca acgggttttc cggtatcgtc ctgcgccttg
     3901 ccgacgaacc cgatcaagga gggcagcgat atatctcgag cgagccgtgc cagttcggtt
     3961 tgattgcgct cggcaaaggc ggggcgctcg atgaggtcct gcggcggata gccgcagagg
     4021 cacagttccg aaaagacggc tatctcggcg ccgccggact tcgcatctcg cgcgagatca
     4081 aggatgcgcg cgcgattccc ttcgaaatcg ccgactgtcg gattgaactg cgccaaagcg
     4141 atcttcattt gaaggcgata gcatacgggg cgcgcgcctg cttttcaatc gcgcgccttc
     4201 cagcgaaatt gcgacggtta agcatgccgg ggcgcaaggc gccgcggaat cgaatccgaa
     4261 aataaaaact cccggagaaa tgcgctccgg gagtcatatg cattcgcttg agatccaatt
     4321 tacacggtga aggaactgcc gcagccgcac gtgctcttta cattcggatt gttgaacttg
     4381 aacccggcgc cctcgagagt ttcgatgtaa tcgatcgaga tgccatcgag atacatctcg
     4441 ctcatctggt cgacgaggac cttcaagccg tcaaactcgt aaacattgtc gctggcctcg
     4501 ttgacctggt tttcgaaggc cattttgtag ctgaaacccg agcagccgcc cccgaccacg
     4561 gctacgcgca ggcccgttgg ctcgggcgtc tgctgcgaga gaatctcttt taccttgttt
     4621 accgccactg gcgtcaactg aaccattttt cagcctcccg gaagcgcgcg ctctgcctcg
     4681 ccgcacgccg ataattccaa gacaattata gcagatttcg ctcccatgcg cagacgcaaa
     4741 ctggcctctt cgaagagacc cgtgacaaaa atgaggcata gctcttaact agcctatcaa
     4801 cctgatgcaa atggctttac actagctgaa taaaaatgaa acatgcgatt acgttgatcc
     4861 cgggagacgg gattggccca gaggtttctg cggctgtcca gaccatactc gccgctgccg
     4921 gcgttcaaat cgagtgggaa caagccagcg cccctagcga tatccatcaa cgtggctccg
     4981 gccgcatgca ggccgaactc gtcgaatcga ttcgcaagaa tggcgtggcg ttaaagggtc
     5041 cgcttgcaac cgggattgcg acgggaccgc ccagcgtcaa cgtgggcttg cgcaaggcgc
     5101 tggacctcta cgcgaacgtg cggccggtga aaaatctgga gggtgtgaag tccagatttc
     5161 aggatattga cctggtgatc attcgcgaga acactgagga tttatatgcg ggactggaac
     5221 atgaggtggt gccaggcgtt gtggagagtc tgaagatcat taccgaaaaa gcgtcgacgc
     5281 gggtggcgcg attcgcattt gaatatgcgc ggaagcacgg gcgcaagaaa attcatggca
     5341 tgcacaaggc caacatcatg aagatgtcgg atgggctttt tctcgcgtcc gtgcggaagg
     5401 tggccgctga atattccgac atcgaataca aagagatgat catcgacaac gcttgcatgc
     5461 aaatcatcct cgatccttat cagttcgata tgctgctgct caccaatctc tatggcgaca
     5521 tcatgagcga cttggcggcg gggctggtgg gaggcctggg cgtggtgccc agcggaaata
     5581 tcggggcgag cgcggcaatt ttcgaggcgg tccatgggac cgcgccggat atcgccggca
     5641 agggagtcgc caatccaact gcactgctga tgagcgcgat catgatgctg gattatctcg
     5701 acgaagtgaa agctgcgcgg cgcatcgagg acgcgctgca taaagtctac cgcgagggga
     5761 aatacctgac tcgcgacgta ggcggcacga cgacaacgct gcaattcacg gaagccgtga
     5821 tcggcgggct ggacccgtag cgacgggcga cttctgtttt cgagttattc agcgtcgaga
     5881 gctacgattg accggacggc gatggtattg cccttcgggt tcaccacacc ggttactttc
     5941 accgcctgag caggatattt tcccgcgaga ttctgcttgt cgagtttgta aactttgtcc
     6001 ttggtccgca gtacgtagac tctgccaaga ttgtccacgc acatgtgcac gcaatcggcg
     6061 ggattgtttc ccatagtgcg cgtcttgagc atctcgtcgt gagaatgcgt ggttgaatga
     6121 acgttaaagg cgcattggga atctgaaatc tcgcccttaa acacctcctg tttctcgccg
     6181 gcggcaagga aagggacaca ggccgatacc atcgcgacta gaaccagtgc ggctctcaac
     6241 tgcgaaggta atttgagcat gatctctcct tgtagtccta cgataatgag tctgcaaatc
     6301 cgggtgacaa gatgcgaaat gaaggcttct aagcgagctt aggagtggcc ccgcgccgag
     6361 tcaattaatc cacccaagcg tgtgcctgct cacttaactg tcccgcgcgg caccaaaggc
     6421 cgcagcgacg tgcagtgttc acgaaaacga tcaacgcaaa ggctcaatcg aagtcactgc
     6481 gattacgttg gacttgggat cgaggacgcc gaccacttct acctttagcc cagcgttctt
     6541 gtcagcgagc tcctgattat cgagcttgta gactttgtct ttcacctgga ggacaaaaac
     6601 tccgccaaga tttttgacgc aatatcgcgc gcaatcggcc gaggtctttc cgattgattt
     6661 tctttccaac atttcccggt gggagcggct gagggaatgc acgttcaacg cgcattgcga
     6721 atccccgatt tcgcctttga aaacctggtc gttgctaccc gcactgacgc cgatcgcggc
     6781 cgatacgatt agtagagcca aaacgataat gctcgagcac ctttccacca ttatctatct
     6841 cctttttcgc taatttcgca ggcattccgt caaagcctgc gcccgatcaa ggcaacacga
     6901 tgaacttcat gtgcattccg tcgtggtcgg ggctgcagac caccgtacag accaccacgt
     6961 attcacccgg ctccgcaggc gcagtcagat taaattcctg cctaccgggc ttgagtagca
     7021 gatcccatcc cggaacgggc gaccgatcgt tggctcgaag caagctgaac ccgtgaatcg
     7081 agccgtcacg gttgtgatcc ttcgctttga cggcggtgat tcgtagcgtc accggggcgc
     7141 cggcgtgcac ggtgataacc ggatctttca ttcccgccat cttgaagcga ctatcgtgat
     7201 ccgcgaggac ttcgatcacc tgaggcgact gcgaccagac gaaatgcgga ctgagtacga
     7261 acaaaaaagc gacggcaacc aggcagatga gtcgcaaact gctaactctc ggatttttat
     7321 ccacatccat tttcgataag cctactgggt gatgtagcgc aggatgtaac gcatgtcgtc
     7381 gtcggtgatt gttgctcgca cgcgcatgtg gcggataacg gttgccatca cgcggggcgg
     7441 atatttgtgt ggcgcttgat ggcatcgtcc acaattggtc gcaaatcttt tctggccttc
     7501 gaaccgccag cgagcatctt cttgcgaaga cgccgaagaa gacgccgcat ttggctgaga
     7561 accggctact tgtgggacag aatccggcgc actggccgcc aattttttgg cttcctgagc
     7621 tgatgccagg ctagagcaca gtgtcagaac accgagcatc acgacggtcg gagccagcca
     7681 tttcgcggtc attcaccttc tccatccccg agaggaatca cgagccggta tgtgagccct
     7741 aaagtccaga cattctcatt tccaccgagg ggcgcaaatt gccggccgta gctgctcacg
     7801 aagcgaaaat tatcctggaa gtaatagttc aggccgaatt caaacatgtt ggtgttcacg
     7861 gggagcacgc tttcgcccgg cgaggagcca acgtagaact gctgcatacg tgcgacagcc
     7921 tgaacatgcc gcatttcgtc gttccagact ggaaactggc ttagccggta cgcggattcg
     7981 atccagtaac cgctgccgtt ctgcgaacgc gcgtattcgc cccggatgtc gagcgggagc
     8041 ggccttggtt gccaggcaaa gtggaatcca aaggcattcg accgattatc ctgaagcagg
     8101 tgctcgaatg atccccctac ttccaggcgt ggccccggca ggaagattcc tacacgctcg
     8161 ccgatggcgc gatcggattc gaaccggttg gccgaactct ccgctgaaaa ataagccgcg
     8221 tagttcagtt cgattttcgg cgtgatattg aatccgccgc gcagcattgc gcccgtgctg
     8281 gcattactcg ggccgacgcc gatgggcaaa attaacggat cgctctgcag gtctcgaatc
     8341 catacggggt ataagcgttc gttgtagatg ccgaaaggag tcaggaagcg gccaatcgaa
     8401 actgtggtgt agcgattggc gatgtaatcc aattgcaggt agtcgacttc cttttccaca
     8461 tctcccttaa aatcgttgct gcccggcggc ggggccaaat cggcttcaaa ggtgcctcgg
     8521 gattcgatca gccagttttg accgattgga atgagaaata tcggcgagac cagaggcccc
     8581 aggtgtggag tcccgccttc gaagctggat atgaaccccg cgcccatggt gaacacgggc
     8641 acaggtttaa ttgcgtggga gtcttcgagc tggccattag cctgtccgac ggccacgccg
     8701 aacagagcgg cggccacgaa caggattcgt agtccggtcg aaagaatcga gttaaagcgg
     8761 gcaaatagca tttgtcggcg accttcagct caagttacct aaggaaatgc agggtctagc
     8821 agacggccca ccctgcatgg caggttatgg cgaggaacag agcaggtcaa caacccgcgg
     8881 cacagaccgg ttcgcgaagt tattaacgat cagggtacag tggacgcatg tttccgttcg
     8941 tttagcgttc ctgcccatgc gtacagtgca catttggaac aattggccct gcgcaacgcg
     9001 tcattgccgg tggccatagg taaactggcg gctgggaggc accatgccca gtcccgccaa
     9061 atttaaggcc gcgtatatat atcggatatt tacctggtcg agtcttgccg tgctcgttct
     9121 cgcattggcg ctcattttca aaaagcctca tgcgcccagc gtaccttctg atccggccgc
     9181 tgccgcgcgc gtggagcaaa aattcgacga agcggatgaa gccgaggcca ctggcaagcc
     9241 ggttgatgtc gatctagatc ggacggaact gaactcctac ctccaccaaa acttgcagct
     9301 cgatggatca caacccgatg tagttgcagc ggggccggca acctccgctt ccgcgagcga
     9361 tacacaacag cccgatccca cagcggcaat cgcaggcaat ggagtgactc ttgaacaggc
     9421 tcagtcatcc gtgaaggacg tcaacgtgga tttagatggc gaccttgtga aggcttatgt
     9481 gacttttgat tttcacggca aagacttggc gctggaactc gatggtcacc tggggacgcg
     9541 ggatggctat ctgaaattcg atccggtggc aggaaaactt ggatccctgc ctctgcctca
     9601 atccacgctg caggccgcag taaacaaaat gatggcttcg cctgagaatc gcgagaaact
     9661 gcgcttgccg ccggatatca gcaatattga ggtttcaaac ggccaggccg tcatctccta
     9721 caaataactc acgcgggcca tatcggcgtg taggcaacgt agagcagcca ataaaccacc
     9781 acgccggtga tcgaaacata tagccagatt ggcagtgtcc atcgcgcgat cgcgcgatgg
     9841 cggtcaaatc gttcccgcag cgcccgcgca agcgtaatga gaacgagcgg tacaattacg
     9901 gccgcaaaaa tcgtatgcga caccaggata gtgaagtaaa ccggacgtat ccaaccctgc
     9961 ccgctgaacc gaatcacgcc ggcatgcatg tgaaaataga gataaaagac gagaaaaaca
    10021 acagagcatc caaacgccga aagcatgcac gcgcggtgcc agcgaacctt gtgattgcga
    10081 ataaaaacgt aaccgcagag caggaaaacg gtgcaggccg cattcagccc ggcattcgca
    10141 gccgggagca tggccaaaaa cggacttctc atcgggagcg gccgctatcc gcccgcatca
    10201 agccacatcc ctctcgcgca ttttgccgcg acgggatgcc tccgctcatc gcgtaatcaa
    10261 ccgattcacg atcagccacg cgagcagcag cggaatgtgc gccaccgtgg cgtgcatcaa
    10321 ccatttcgcg cgcgcatttg tgcgcgtgcg attcgcccac agccccactt gcagcagaat
    10381 cattcccaga ataagcgcgc caaaaaagta atatttcccc gccattccca gcacggcagg
    10441 aagggcgctc acccagacca gaaccgcgga ggtccagatg atctgcctga aagtcgagtc
    10501 cccttttcgg tccacgaccg gcagcatgcg aatgcccgcg cgcgcgtagt cttcgcggta
    10561 catccacgcg atcgccatga agtgcggaaa ttgccaaaaa aacaaaatcg caaatagcac
    10621 ccagccacca agggaaagcg aacctcgcgc ggctgcccag ccgatcagcg gcggcagcgc
    10681 tcccggaatg gcccccaccg cggtggaaag cgtggtgcgc gtttttagcg gcgtatacaa
    10741 tcccagataa agcacgcacg tagccagagc cactagcgcc gccaatccat tgaccagcaa
    10801 tgccagccat gctgcgccgg ccacgattgt gcccagtcca aaaatcagca cgtctttggg
    10861 ctgcaacagc cccgaaggca gaggtcgcga cgctgtacgc cgcatgaccg cgtccatctt
    10921 tcgttcaaca tattggttca gtgcggccgt gccgcctgcc accaggagcg tgccccccag
    10981 cgtgtgcagc atcagcaccc aatcaagcgg accggtcgaa cccaggtaga atccggcaac
    11041 cgtggtgatc actaccagga aggtgacgtc cggcttggtc aagacaacat acgcccaggg
    11101 cttgtcgaac gtggatagtg tatcggctcg agtattgccc attgcttctc tagacccccg
    11161 ccctctcggg cgacttctcg cctccactgc tgatcactcg cgactcgacc gccatcggag
    11221 tggcgcggcg aatcaggcgg accgaagtga gcgtcacgat cacggaagcg gccaatgtga
    11281 gcgcgccgcc caaaacgtgc gccactgtga gcgaaacgta cgtaaatgtc ggctgtaccg
    11341 catctcgtgc cgccgcaact gcccaccatg ccgcgaaccc tagcagaatc tgaataccaa
    11401 aaaatgagtg caacagtatt gttgcacggc gaagaggccg ctcctgccga aaccgcttct
    11461 tggcgacaac gcctgtccac acaaccatta cggtgacgac tcccgcgcca accaggtgcg
    11521 gatcgattcc aaaggcgccg tggcgaaacg ccgcgcccag gacgagctgc accagaatgg
    11581 cggctgtggt ccacgcagcc agggtgcgca aacgaggcga gccgcgatca tcaagttccg
    11641 ggtgatcgct ttgccaccag ccactcagat aaaacgtaag tccgacgatg gtgatgaaaa
    11701 atatttgcgc a
//
LOCUS       DATNDT010000180         8346 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2019230, whole genome shotgun sequence.
ACCESSION   DATNDT010000180 DATNDT010000000
VERSION     DATNDT010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8346)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8346)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8346
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2019230"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VN788_05890"
     CDS             <1..233
                     /locus_tag="VN788_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20096.1"
                     /translation="HRLMRYPCSYMIYSDAFDALPDELKSAVYERMWEILSGQEKEKK
                     YAKLTFADRKAVVEILRDTKKRLPDYFQPIRR"
     gene            complement(237..2492)
                     /locus_tag="VN788_05895"
     CDS             complement(237..2492)
                     /locus_tag="VN788_05895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXU20097.1"
                     /translation="MAASAPLIGQTVSHYHILKQVGAGGMGVVYEAEDSRLGRRVALK
                     FLPPEMAEDSQSLERFQREARAASSLNHPNICTIHAIEQHELRYFIVMELLEGQTLAQ
                     KMTKQPMDIDKLLPLSIQIADALESAHAKGIVHRDIKPANLFLTNHGDVKILDFGLAK
                     ITANEIAQTTSEGQRATMAGGGELTMPGSAVGTVSYMSPEQARGQLVDTRTDLFSTGT
                     VLYQLATGTLPFQGDTSAVIFEAILNRDPKPAGDVNPSLPPDFVRILEKALEKDRNLR
                     FQTATDLKTDLSRLKRDLEAHKRRAKEKTDSDPGTQKKMGKSVAVLYFENLSGAKEDE
                     YLRDGITEDIITELSKIRGLNTFSRPTVLGFRDKAVTPAQIGQQLGAAYVLTGTLRRS
                     GARVRISAQLVDTSTDFPLWSERFDREMKDVFEVQDEMARKIAEALRVTLSPQELEAL
                     ADKPTENPQAYDQYLRGKRYARRQTRQDVEFALQMFENAVSLDPSFALAYAASANACA
                     MYYSIFTRDEVWIERAREASGRAVALRWDLPETHVSQAWVLYAAGLHDEAVRMVKKAI
                     ERRRDCDGAYYLLGRALFAAGRYQEVLDVAEAAIEASGEDYNVYVPIMNSFGAMGKEE
                     AGRNMSHRVVVALEKHLKEVPEDARARVLLGGKYAELGRQEDALRELNLAVTLRANEA
                     SILYNAACVYCILKRKPEALDALRKAWEAGSKDSVWARRDPDLSMLHGDPEFERLYPE
                     KTASQPATNSR"
     gene            complement(2616..3680)
                     /locus_tag="VN788_05900"
     CDS             complement(2616..3680)
                     /locus_tag="VN788_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HXU20098.1"
                     /translation="MEKLAHFANSLESWQQRAETIRATILAGAHLTPLPERTPLHPIF
                     ADRRVRGSYSVEDVAFESVPGLYVFGNLYRPLRAKTRGSVPAILVAHGHFKQEGWYAR
                     TRPENQLLCATLAQMGAVVFTYDMVGYGDSTQLPHNTRNVLQIQLWDSIRALDFLESL
                     SEVDATRIGVTGASGGASQIILLAAVDPRVQASMPVVMVSSRYSGRERCEDGMKVRDV
                     PGPEDTNNAEIAAVVAPRPLMIVSDGADWTRHFPDEDFPYIQRIYDLYDAIGNVHNRH
                     LPNEHHDYGPTKRRIAYRFFSRSLGLAAPPKRETAPIESQADQAVRNWVDLPLTFSGD
                     FEAELTKSLPDQSASNNVGQ"
     gene            4248..5018
                     /locus_tag="VN788_05905"
     CDS             4248..5018
                     /locus_tag="VN788_05905"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXU20099.1"
                     /translation="MSTFSRGYGGYRFDLGGYFPRAVKTLCIACISVFVLQELSELLF
                     HSAGREFWLQWFGLVPYAVVHGGRVWQLFTYLFLHAGILHILLNLLFLAMFGADLEHT
                     WGSRRFYGYFFLCGVGAGVFNVAVKTMVDPYGLGATIGASGAIYGVLLANAVLFPDRR
                     VWMFPLPVTVSMKIFVAIMVAIEFFGTLGSSGDNVSHICHLGGMLVGYIYLRRGSYLF
                     NFRNSFSDWKRRRLRKKFEVYQRDHSDKPPSRPDNWVN"
     gene            complement(5434..6903)
                     /locus_tag="VN788_05910"
     CDS             complement(5434..6903)
                     /locus_tag="VN788_05910"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXU20100.1"
                     /translation="MARGIPRIALTIFAGALVCAFPIRAQSHPMPAPPAQAPSTASAA
                     SSIVGMENEAISWLQGLIRINTTNPPGNELKAAKYLAAILEQEGIHADVFESTPGRGF
                     LVARLSSSAVPNPSRALLLMGHLDVVGVDRSKWKVEPFSGAIQDGYLYGRGAIDDKGM
                     TIANMAVFIALKRSGARLDRDVIFLAEGDEEAGGDAGMTVAVEKHWDKIAAGFAINEG
                     GRVILKNGKVQYVGVQASEKVMVNVDVVATGTSGHASMPLKDNPVAHLASAIAKISTF
                     EAPVQFNSVTRAYFDGLAPIEDEETGKWIKALQTSDRAAHAARWISDANPTWNAMLRD
                     TVTPTMLQAGIRPNVVPSEARGVLNVRLLPGNMIDPLVAKLQQMVNDPQVKLEIEPGG
                     GEAAPSSSLTSDLFASITRVADKDFPSAPVLPYMSTEATDSTPLRMRDVQAYGLLPFP
                     MTQDDLSRMHGDNERIQLDNFRKGLEFLNDIVVDFAVAK"
     gene            7067..8176
                     /locus_tag="VN788_05915"
     CDS             7067..8176
                     /locus_tag="VN788_05915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU20101.1"
                     /translation="MHVKAYHLAILAVVLIAAIAVPRWDVSAQNNGPQQSAPPQQPEP
                     VKPPTSSAPNAPPQRQQPPPPQVAISVQSNLVTVDAIVTDQDGNLVTGLKRENFRVLD
                     DGQPQQITNFAPTDAPITVVILMEYSSTYWGYYQGYFGYKAQLWADGFLQALKPQDWV
                     ALKTFDLRTTLQEDFTQDKRSIDEAIRTLGFPSFHEAVLFDAVYETLNQLRDVKGKKS
                     ILLLATGFDTFSKHNLDQIINRLKESETTIFAVGMGEEIDLYSPGGAGIGYMQAKNQL
                     STFARMTGGFAFFPRFQGEMPDIFNTVASYLRSQYTLVFSPSTPQDGKYHKIAVQILD
                     DDGNPLMLVNKKGKKKKVVVTAREGYTAPHSSAGG"
BASE COUNT         1740 a         2424 c         2359 g         1823 t
ORIGIN      
        1 aacatcgcct gatgcgttat ccgtgcagct acatgatcta ttcagacgcc ttcgacgccc
       61 tgccggacga acttaaatcg gccgtctatg aacgcatgtg ggaaatttta tctgggcaag
      121 agaaagaaaa gaaatacgcc aaactcacgt ttgccgatag aaaagccgtc gtcgaaattc
      181 tgcgcgacac caaaaagcgc ctgcctgatt acttccagcc gatcaggcgc taggaactag
      241 cgcgaattcg tggcgggctg cgaggcggtc ttctccgggt agaggcgctc gaactccggg
      301 tcgccatgca acatgctgag atccgggtcg cggcgcgccc aaaccgaatc cttagatccc
      361 gcctcccagg ccttacgtag cgcgtccagg gcttccggtt ttctcttcag aatgcagtac
      421 acgcaagccg cattatagag aatcgaggct tcgttggcgc gcagggtgac tgccagattg
      481 agctcgcgca gtgcatcttc ctgtcgtccc agctctgcgt atttgccgcc cagcagaacg
      541 cgagcccgcg catcttcggg cacctctttc aagtgctttt cgagcgcaac gacgaccctg
      601 tgggacatgt ttcgtccggc ttcttctttg cccatcgcgc cgaaagaatt catgataggc
      661 acataaacgt tatagtcctc gccgctcgct tctatggcgg cctcagcaac atccagaact
      721 tcttgatagc gtcctgcggc gaaaagggcg cgccccagca ggtaataagc gccatcgcag
      781 tcacgtctgc gttcaatggc cttcttgacc atccgcacgg cttcgtcgtg cagtccggcg
      841 gcgtagagga cccaagcctg gctgacgtgc gtttctggca ggtcccaacg aagggcgacg
      901 gctctgccgg aagcttctct cgcgcgctcg atccaaacct catctcgagt gaagatggag
      961 tagtacatgg cacaagcgtt ggcgctggcc gcataggcca gggcaaagct tgggtccaga
     1021 gataccgcgt tttcgaacat ctgaagagcg aactcgacgt cctgccgggt ctgccgccgc
     1081 gcgtaacgtt tgcctcgcag atattggtcg taggcctgcg gattctccgt gggcttgtcg
     1141 gccagtgcct ccagttcctg cggcgagagc gtcacgcgca gggcctcagc aatcttgcgg
     1201 gccatttcgt cttgcacttc gaaaacgtct ttcatctcgc ggtcaaagcg ttccgaccag
     1261 agcggaaaat cggtgctcgt gtccacgagc tgagcgctga tgcgcacgcg ggcacccgaa
     1321 cgacgcagtg tgccggtgag gacataggcc gcgccgagct gctggccgat ctgcgcagga
     1381 gtaaccgcct tatcccgaaa gcccagaacg gtggggcgcg aaaacgtgtt cagcccccga
     1441 atctttgaga gctcggtgat gatgtcctcg gtaatcccgt cgcggagata ttcgtcttcc
     1501 ttggcgccgc tcagattttc aaaatagagc acggccacgg atttccccat tttcttttgg
     1561 gtgccagggt ccgaatcggt cttttccttc gcgcgccgtt tgtgtgcttc cagatcgcgc
     1621 ttcaggcggc tgaggtcggt tttcaggtcg gtcgcggtct ggaagcgcag attgcgatcc
     1681 ttctcgagcg ccttctccag gatgcgcaca aaatccgggg gcagcgatgg attcacgtct
     1741 ccggccggct taggatcgcg gttcaggatg gcctcgaaga tcaccgctga ggtgtctccc
     1801 tgaaagggga gcgttcccgt ggccagttga tagagaacgg ttcccgtaga aaaaaggtcg
     1861 gtgcgggtgt cgacgagttg gccgcgcgcc tgctcgggcg acatgtagga gacggtgccg
     1921 acggccgacc cgggcatggt aagttcgccg ccgccggcca tggttgctcg ctgtccctcc
     1981 gaggtggtct gcgcgatttc attcgctgtt atcttagcca agccgaaatc caatatcttc
     2041 acgtcgccgt ggttggtaag aaaaagattg gccggcttga tgtcccggtg aacgatgccc
     2101 ttggcatggg ccgattcgag agcgtcggca atctggatgg agagtggaag aagcttgtcg
     2161 atatccatcg gctgcttcgt catcttctgc gccagggttt gcccctccag caattccatc
     2221 acgatgaagt aacgaagctc gtgctgctcg atcgcgtgaa tcgtgcagat gttggggtga
     2281 ttgagggagg aagccgcccg ggcctcgcgc tgaaagcgtt ctagcgattg gctatcttca
     2341 gccatttccg gaggaagaaa tttcagcgcc acgcgacggc cgagccgtga atcctcggct
     2401 tcgtacacta cgcccattcc gccggcccca acctgcttga ggatgtggta gtgcgagaca
     2461 gtttgtccaa tcagaggtgc agaggccgcc atgaatggca catcttagat gctcgcggca
     2521 ggcgagtcaa cgacgcgcga cgctgagcga ggcgtcatgc gtatctgata tagggcgggg
     2581 aaaagtgata caacgcgtcg acctgtacca ggatcttact gacccacatt attactagcc
     2641 gactgatcgg gcaggctctt cgtcagctct gcctcaaaat ccccggagaa tgtgagcggc
     2701 agatcaaccc aattgcgaac cgcttgatcg gcttgggatt cgatcggcgc ggtttctctc
     2761 tttggaggag cagctaaccc tagagaacgc gagaagaatc gataggcaat acgccttttg
     2821 gtggggccat aatcgtggtg ttcgttgggc agatgccggt tgtgaacatt cccgatcgca
     2881 tcgtacaggt catagatccg ctgaatatag gggaaatcct cgtcgggaaa atgcctcgtc
     2941 cagtctgccc cgtcagaaac aatcatgaga ggacgtggcg caacgaccgc cgcaatttcg
     3001 gcattgttgg tatcttccgg accaggaaca tcgcgtacct tcatgccgtc ctcgcatcgc
     3061 tcgcgcccgg agtagcgaga cgaaaccatg acgaccggca tactcgcctg gacccgcgga
     3121 tccaccgcgg cgagaaggat aatctgcgaa gctccgcccg acgcgcccgt aacgccgatt
     3181 cgcgtggcgt caacttcaga gagggactca aggaaatcga gagctcggat gctatcccag
     3241 agctgaatct ggagtacgtt tctcgtgttg tgcggaagtt gcgttgaatc gccgtagccg
     3301 accatgtcgt aggtgaaaac gacggcgccc atctgcgcaa gcgtggcgca caaaagctga
     3361 ttttcggggc gcgtacgagc ataccagccc tcttgcttga agtggccatg cgcaacaagg
     3421 atagcaggca ccgagcctct cgttttcgca cgcagcggcc tgtagaggtt cccaaataca
     3481 tagaggcccg gcacggattc aaaagcgacg tcctccaccg aatacgagcc gcgaacacgg
     3541 cgatccgcga aaatcggatg aagcggtgtg cgctcaggaa gaggagtaag atgtgcgccc
     3601 gcaagaatgg tggcccgaat cgtttcggcg cgctgctgcc aggattcgag agaattagca
     3661 aaatgcgcca acttttccat ttgctgcgca gccagatgcg gagcaaagac gacgagcgca
     3721 gcagcacaaa ccaaaaagcg acaccggaac agcctcaccg cgggtaagta catcgccgta
     3781 tcctcacttg gttggaatca aagaaagcca ctggacaaag gttcaggaga gaatcgctct
     3841 gatcttcgtc gcctcctcca ggagtcacgg attggattag ccgaggagga tgagagatgc
     3901 aggttctcca acatttttaa tcattgggcc agaggggacc gacccatgcc ttgaagaccc
     3961 ggccactgca ccggcgagcc tgcctttgcg gcgctgaaag gggctttcgg caatcgatac
     4021 cttgatgctg gcctcggtac aacattggcg gtgactgttt cgcagaagca aaggttgcag
     4081 gggtggaagg gcggggcttc agccccgccg tgagtcggcc gatatgagac gagctttagc
     4141 cctgaaccca ttgccgattc gcccttccac gcattctatc ccgcgttccc gtcattacct
     4201 ctgcagcctt ttgactacgc accccccagc ctttagactg gtgctctgtg agcaccttct
     4261 ctcgcggtta tggcggctac cgcttcgacc tcggcggcta ctttcctcgc gccgtcaaga
     4321 cgctttgtat agcgtgcatc agtgtcttcg ttctgcagga attgtcggaa ttgctttttc
     4381 acagcgccgg aagggagttt tggttgcaat ggttcggact cgtgccttac gcagttgtgc
     4441 acgggggtag agtatggcag cttttcactt atctcttcct gcatgcggga attctccata
     4501 ttctgctgaa tcttttgttc cttgcgatgt tcggcgccga cctggaacac acctggggct
     4561 cgcggcgatt ctatggctac tttttcctct gcggagtggg tgctggagtt ttcaacgtcg
     4621 ctgtcaagac aatggtggac ccttacggtt tgggcgccac tataggggct tcgggcgcaa
     4681 tctacggtgt gctgctggcc aatgccgtgc tgtttcccga tcgccgcgtg tggatgttcc
     4741 ccttacccgt aaccgtatcc atgaagattt ttgtcgcgat catggtcgcg atcgaattct
     4801 tcggcactct aggttcttcg ggcgacaacg tcagccacat ttgtcacctg ggcggaatgc
     4861 tggtgggcta catctatttg cggcggggat cctacctgtt caacttccgc aattcttttt
     4921 ccgactggaa gcggcgccgc ctgcgcaaga agttcgaggt gtaccagcgc gatcacagtg
     4981 acaaaccgcc cagccgcccc gataattggg ttaattgaga gcgctaggaa agcatcacct
     5041 ggcgccgtca tcctgccctg ccctggcgga gcaagggagc ggagccccgt gcgtccttgg
     5101 cgcccgcgcc catcaacgtc atcctgagcg gagttgtgcg cgcctttgcc tttcccgcgt
     5161 ctttcgcggg cgcgcaacgc agtcgaagga tctctcttgg attgaaaata tgccgcagct
     5221 caaattgaag agtcgttccc cgactgcatg tccactcgtc tcgcgcaaaa ccaaaagcgc
     5281 ggccggtccg ctcaaaataa cgcccgaatc cacacccacc gtggacgatt tttctttaca
     5341 ccaacaacta cttgcgcgat tccaccccac ggtgtaaaga ttttcctcgt atcctcggat
     5401 tctgcccaca ccaccgcctc cgaggtcctt ccactatttg gcgacggcaa aatcgacgac
     5461 tatgtcgttg aggaattcga gtcccttgcg aaagttatcg agctggatgc gttcgttgtc
     5521 gccatgcatc cgtgagagat cgtcttgggt catcggaaat gggagaaggc cataagcctg
     5581 aacgtcccgc attcgcagcg gtgtggagtc cgttgcctcg gtggacatgt acggcagaac
     5641 tggtgccgac gggaagtcct tgtccgccac tcgcgtgata ctggcgaaga gatcggatgt
     5701 gagggacgaa gacggcgcgg cttctccgcc cccaggctcg atttccagtt tcacctgagg
     5761 atcgttcacc atctgctgca gcttggcgac cagcgggtca atcatgtttc caggcagcag
     5821 ccgcacgttg agcacgccgc gcgcttccga cggcaccacg ttcggccgga ttcccgcttg
     5881 cagcatcgtg ggcgtgaccg tgtcgcgaag catggcattc caggttggat tggcgtctga
     5941 gatccatcgc gcagcatgcg cggcgcggtc cgaagtttgt agcgccttta tccatttgcc
     6001 ggtttcttca tcttctatcg gcgccaagcc atcaaaatac gcgcgcgtaa cggaattgaa
     6061 ctgcaccggt gcttcgaacg tcgatatttt tgcgatggcg cttgccaaat gcgcaacggg
     6121 attgtccttc agcggcatag acgcgtgccc agacgttccc gtggcgacca cgtccacgtt
     6181 taccatcact ttttcgcttg cttgcacgcc cacgtattgc accttaccgt tcttcaaaat
     6241 cacgcggccg ccttcgttga tcgcgaagcc tgcggcaatt ttgtcccaat gcttttcgac
     6301 cgcaaccgtc atgccggcat cgccgcccgc ttcttcatct ccttctgcca ggaaaatcac
     6361 gtcgcgatcc aaccgcgctc ccgaacgttt tagcgcaatg aaaacggcca tattggcgat
     6421 ggtcatccct ttgtcgtcaa tcgcacctcg gccataaagg taaccatcct ggatagctcc
     6481 cgaaaatggt tccaccttcc attttgacct gtccacgccg actacatcga ggtggcccat
     6541 cagcagaagc gcacgcgaag gattaggcac cgcgctggac gaaaggcgcg ccaccagaaa
     6601 cccgcgtccc ggagtgcttt cgaagacatc cgcatgaatc ccttcctgct cgaggatcgc
     6661 cgcgagatat ttggcggcct tgagctcatt gcccggagga ttggtggtat tgatgcggat
     6721 caagccttgc agccaggata tggcttcatt ttccatcccg acgatgctcg acgctgctga
     6781 cgcagttgaa ggcgcttgcg caggcggagc gggcatgggg tggctctgtg cgcgaattgg
     6841 aaaagcgcaa accagcgcgc ccgcgaagat cgtcagggct attcttggaa ttcctcgcgc
     6901 catccttttt gcctcccgtg gaatctgaaa gagcatcgcc aagcgcacca aatcaatact
     6961 atcatgtgga ttcgtcatgc cccggataga cagcgcgctt cgtttctgga ttgcaatcaa
     7021 acatccagac taagatgtaa gtgcatcgaa gctctgtgag gcaaatatgc acgtgaaggc
     7081 ctatcatctc gccattctcg cagtggtcct gattgctgcg atcgcagtcc ctcgttggga
     7141 tgtatccgcg caaaacaatg ggcctcagca gtccgcgcct ccgcagcaac ccgagcctgt
     7201 aaagccgccc accagttcag cgccgaacgc cccgccgcag cggcagcagc cgccaccgcc
     7261 gcaagtggcg atttccgttc aatccaacct cgtgactgtg gacgcgatcg tgacggatca
     7321 ggatggaaac cttgtcacag ggttgaagcg agagaatttc cgagttttag acgatggcca
     7381 gccgcagcag atcacgaact tcgcccccac cgacgctccg attaccgtgg tgatcctgat
     7441 ggagtacagc agcacgtatt ggggctacta tcagggctac tttggctaca aagcccagct
     7501 ctgggcggat ggtttcctgc aggccttgaa gccccaggat tgggtcgcgc tgaagacttt
     7561 cgacctccga acgactcttc aagaagactt cacgcaagat aagcggtcta tcgacgaggc
     7621 tatccgcacc ctgggtttcc caagcttcca cgaagcggtt ttattcgacg ctgtgtacga
     7681 aacgttgaac caattgcgcg atgtgaaagg caaaaagtca attttgctgc tcgcgactgg
     7741 gttcgacacg ttcagtaaac acaatctgga ccagatcatc aatcgcctga aggaaagcga
     7801 gaccacgatt tttgcagtcg ggatgggcga agaaatcgat ctgtattccc cgggcggagc
     7861 aggcatcggt tacatgcaag cgaaaaatca actctcgacg ttcgcccgca tgaccggcgg
     7921 cttcgcattt ttcccgcgtt tccagggcga gatgcccgac atcttcaata ccgtcgcatc
     7981 ctacctgcgc agccagtaca cgctggtttt ttcgccgtcc acaccgcagg acggcaaata
     8041 tcacaagatc gcggtccaga ttttggacga cgacggaaat ccgttgatgc tggtgaacaa
     8101 aaagggtaag aagaaaaaag tggtcgtgac tgccagggaa ggctacacgg cgccgcattc
     8161 cagcgcgggc ggctaagtcc acactcaggc cggcgccgca atttatcggt ggacgcgccg
     8221 cttcacgaat tcatccagca gaacctgcgt gaggcgcgat tgatcgtggc gcgccatatt
     8281 tcctcgccgc agaaaatttc cagtaacgta gctcaagccc atctgcgaaa gttcgtcgag
     8341 tgaggg
//
LOCUS       DATNDT010000181         9879 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_813287, whole genome shotgun sequence.
ACCESSION   DATNDT010000181 DATNDT010000000
VERSION     DATNDT010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813287"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="VN788_05920"
     CDS             complement(<1..1087)
                     /locus_tag="VN788_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HXU20102.1"
                     /translation="MRLNGAAIGAALSLMLLALPLPSNSVLGQDRPTAPASITVGSDG
                     SVEIPAQSVPMSIYLSPEAQAYVTKHLQDMQNPQMLKGEGGVPRFMKPYLDRDYEEFP
                     LDKKDTTIAGVHAFIYTPKAGVPARNKHRVLIDLHGGGFSGCWPACAELESIPVSFLA
                     QIEVISLDYREAPANKFPAASEDVASVYRELLKTHNAKEIGIYGCSAGGMLTAESMAW
                     FQAHALPAPGAIGILCASASGTFGGDSSYTANWLGEARMPAGARGASVRGGPARPGYF
                     DGANMKDPLISPADSPEVLAKFPPTLVVTGTRGFEMSSAVYTHSRLVKAGVDAELHVW
                     EGLFHGFFYNADVPESKDCFNVILKFFD"
     gene            complement(1255..1581)
                     /locus_tag="VN788_05925"
     CDS             complement(1255..1581)
                     /locus_tag="VN788_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20103.1"
                     /translation="MVATKNGTNFVSSPDYEKAIDELVNRDFEPEAAFYQLLIGVTAY
                     FLCGIDPHCEDLDVFDAFISENMPVIRAAIDRVKSSLPQIRYAEEAWIKSASPSFADR
                     PATGTG"
     gene            complement(1632..3245)
                     /locus_tag="VN788_05930"
     CDS             complement(1632..3245)
                     /locus_tag="VN788_05930"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HXU20104.1"
                     /translation="MKRMPVVLAFLSAVVVGLFLTVPSRTTKGATPVSISEGLQEPIQ
                     VDGGLITGTPTPQWTYGVRLYRGIPYAAPPVGELRWHPPQPVVPWQGVKRADHFSPVC
                     MQMPTDTEGNAWREGLVPVSEDCLYINVWTPAKTASDRLPVMVFIYGGGNTRGAASEN
                     QYDGTYVAKKGVVFVSFNYRLNVFGFLAHPDLTAESEHHSSGNYALLDQIAALQWIQR
                     NISRFGGDPSTVMIFGHSAGASNVSSLLASPLAKGLFERALMQSGENLGKGVPLSAAE
                     KNGEKFAESLGAHSIADLRKLPAEQILKASRAPMSLIVDGWVLPQDVYSTFTEGKQND
                     VPVIVGSVGSDAPGPAPGPAKAADVPAYAQKTFGDLADQYLKVFPADTDEQATKSALA
                     FRTNRAEANARMLARLQVKTGKSKAYWYIFTHLSPFPEGLVWGGRPAKEWGAYHGSEI
                     VYVFDAFPLEDWAWRPVDYKLGDTVSSMWTNFVKTGDPNGPGLPKWPAYDPETDVLMN
                     FGDNPQAQPAPYKTALAFFDTLDREQRGK"
     gene            complement(3342..4511)
                     /locus_tag="VN788_05935"
     CDS             complement(3342..4511)
                     /locus_tag="VN788_05935"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HXU20105.1"
                     /translation="MRLKISALGVLVFLFGFSAMAQSPNDKWLPNDMDRPMPPAITFS
                     SAGVPSDAVVLFDGSNLNAWVGRGGSAPEWDIKDGALIVKPRTGSITTKQSFGDAQIH
                     VEWAEPLPAMGDGQNKGNSGVIIMGVYEIQILDDINNKTYADGIAASVYGQYPPLVVA
                     ARNPGEWQTMDILFRRPRFDSTGRLITPAHITIIQNGVYVQDDVTVTGPTGEPRAPYV
                     VGPEAAPLSLQDHGEPVRYRNLWIRPLAPREPDLSYTALTPYSHLNASDLPAYAGHYQ
                     VNENSSVDIQVSGNSLTAQVHGGFGGLGRGGRGGPAGRGRGAAGPTPQATAPTGPPPG
                     FQLPAMPLVPVNKDMFINAHLAGYLLITFTRGADGQVNGLTMLQGNNYRYAQKAQ"
     gene            complement(4631..6181)
                     /locus_tag="VN788_05940"
     CDS             complement(4631..6181)
                     /locus_tag="VN788_05940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU20106.1"
                     /translation="MAKDGITRRHFFIGSLLAGAIPAAGWGSVPSLKMLGYKSPNEKL
                     NIAGIGAGGQARADIGGVSGENLVAFADPDSVRGGQTFQQYPNVPKYTDFRKMFDKEE
                     KNIDAVTIAIPDHMHATAAMWAMERGKHVYVEKPLTRTPWEARMLTEAAARYNVATQM
                     GNQGYSNDGARVAAEIIWSGEIGNVTEVHAWTNRPIWPQGMTEIPDEEKTPDTLDWDT
                     WIGVAAMRPYTSGGATYRQQHRTQFGFYQPFNWRGFLDFGSGPIGDMALHILGAPNMA
                     LLLGNPTSVELIKQEGKNTLSYPNSTVTRFEFPSRGSMPPLTLYWYDAQRGAAYKPKD
                     LPEGEPLIGGEDAFGAPTPRNPFSAAFNAAAANGGNPPAGPARGGRGGAIPAGMQEAN
                     GAVFVGEKGYLTTDTYGAKVRLLPLERHKAYKLPPEVLTRSPEHHLDWLRACKGGERA
                     CSDFSVAGPFTEWILLSTIAMRVDGKLQWDSTKLRFTNSEEANRYVTPEFRKSWSQVW
                     AGKKLNKA"
     gene            complement(6220..>9879)
                     /locus_tag="VN788_05945"
     CDS             complement(6220..>9879)
                     /locus_tag="VN788_05945"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU20107.1"
                     /translation="DATGAVVPGAHVTLTNTGTNISQSAQTGSDGNYTFPLLQVGTYV
                     VKVEATGFQTFVAPSIPLSAGDRVRVDAKMIVGSQTTTVEVQAAAPALQTDTSNVSVL
                     VPSQQVEDLPLNGRNIITLVTRTAGANEGSPGSIVAGNRPDDRRQNSQISVNGQQDTQ
                     NNNLIDGMDNNERFIGSIGVRPSVDAIQEVNVQTNNYDASVGRTGGAVIDIITKSGTN
                     DLHGTVYEFFRNKVLNANPNYNFAQALNPGSKSPAAPNPAFRQNQYGGTLGGPIKKNK
                     TFFFGDYEGLRYATGKSAALFNVPTYCERGLGNSSSPFAKACPDGGTAPGDFSDIKLV
                     SSPGGSTTQPNTPGPNLSGLPLCTGSSAPGTCLSPLGLAYFNMYPLPNTGAAGAILQN
                     YTSTTENTQNSQTWDVKVDQHFSDKNTMYLRYTHNGESTLIGNGFPAVTINPATGAAV
                     SAGTSGGVLINPTVTTFSGTNSEAQDQGSGGFVHIFNSNLVLNLKFSIFQNSIYSYTP
                     NQGKPIMNELGFPCNATACIGSPSSLLGRPNPNFIIMSASISGANGAGGYTTIQDLSA
                     IPLEEWDTDFHYFGGLTWTKNAHSVRFGASLIRRRAAIDQASTPSMSFTGAYTGVAPG
                     DLLEGLDVSLNHPVALVKQQFRMWEPSVYVQDDWRFRRWLTLNLGVRYDIFTPFTEAN
                     GQISNYNPYIGLLVSPSLPGPQHSSPTANVPTRYNALAPRIGFAATLAHNTVIRGGFG
                     LSFFPVNYESPYYEKNAPFSFRGSCQAQNEVGTNVSCATAQFGSGAVGQFSNGLTVTY
                     GLPTSNPGSNVGKTGGALMAAGLPSPYFDVTLATNPANYAGTGIQAIQTTDFKQVYLE
                     QFNLAIQKQFGANVVSVGYVGQIGRHIEAFDTGENQNLAANPTENITGLPLTVGGMSN
                     EGFGVLQGYPYLKTVSVSEPAQIGTSFYNGLQSSFQRRFNHGLTVNFSYVWSHMLDNI
                     AGNRACVVSMFATPEPCWYDLSNGAGPSLASATSPGACGAEPTVCHATWGWQKADWGN
                     DSMNVFDRFTWGLNYQLPFGKSDTGVKAGFIKGWSTNFSGSWQTGIPFSVTDSVSNSQ
                     IAGSQYPDQICSGKLANPTIQHWFNYNCFVQPVAGTEGDMHPGQFKGPSLTRFDASLF
                     KDFALTERVKMQFRTEIFNLFNTPNFNGPSGTSIAFKNGAVDLTGSHATTGQISSMNA
                     AWNPRQIQFALKFLF"
BASE COUNT         2174 a         2756 c         2689 g         2260 t
ORIGIN      
        1 tgtcgaaaaa cttcagaatc acgttgaagc agtccttcga ttccggcacg tctgcgttat
       61 agaagaatcc atggaagagg ccctcccata cgtggagttc ggcatccacg cctgccttca
      121 cgagtcgaga gtgcgtataa acagccgaac tcatttcaaa cccacgagtt cccgtaacaa
      181 ctagggtcgg tggaaatttc gcgagtactt ccggtgaatc ggctggggaa atcagcgggt
      241 ccttcatatt ggcaccgtca aaataacctg gccgcgcagg cccacctctc acagacgcgc
      301 ctcgcgctcc cgccggcatt cgcgcttcgc ctaaccaatt ggcggtataa gaggaatcac
      361 caccaaacgt gccgctggcc gaggcgcaaa gaatcccaat cgcgccaggc gctggaagag
      421 catgagcctg aaaccacgcc atagattcgg ccgtcagcat tccgccagcc gagcatccgt
      481 aaatgccaat ctccttagca ttgtgggtct tgaggagttc gcgatacacg ctcgcaacat
      541 cctcgcttgc ggcgggaaac ttgttagcgg gcgcctcgcg ataatcaagg ctaattacct
      601 cgatctgcgc caaaaacgaa actggaatcg attcgagttc cgcacaggcg ggccagcaac
      661 cggaaaaccc tccgccgtga agatcgatga gaacgcgatg tttgtttcgc gcaggtacac
      721 cggccttagg cgtgtaaatg aatgcgtgta caccagcgat cgtcgtgtct tttttatcga
      781 gcggaaattc ttcatagtca cggtctagat agggcttcat aaagcggggc actccgccct
      841 cgcctttcaa catttgagga ttctgcatat cctgcagatg cttcgtcacg tacgcctgcg
      901 cttcgggact gagataaatc gacatgggaa ccgattgggc cgggatttcc acactcccgt
      961 cagaaccgac ggttatgctc gcaggggccg tggggcggtc ctggcctaag accgaattcg
     1021 acggaagtgg caaggctaac aacattagtg atagggcagc tcctatggct gcgccattta
     1081 gtctcataaa cgcctcctca caaatcgtaa atcgtcgcaa tttcgagctc ttgtctgata
     1141 cgacggcctc ggccgcatac aatgactatc actcccgata gcggcatgct gagatgcaaa
     1201 cgaacaatgt caaccacgcg ttctcctgcg gcgcgagcgg ttttgccgat gtattcaacc
     1261 cgtacccgtt gcaggcctgt cagcaaacga aggcgaagcg gattttatcc aggcttcctc
     1321 tgcgtaacgt atttgtggca gggagctttt cacgcgatcg atcgctgcgc gaataaccgg
     1381 catattttcg ctaatgaagg cgtcaaagac atccaggtcc tcgcagtgag gatcgatacc
     1441 gcacaagaaa tacgcggtaa ctccgattag cagctggtaa aaagctgcct ccggctcgaa
     1501 atcgcggttc acaagctcat cgattgcctt ttcataatca ggagaactga caaagttcgt
     1561 gccattcttt gtcgctacca actttcttcg actcctcccg tgtcgtcggc gcagtaacga
     1621 cattgcaggg actatttgcc tcgctgctcg cgatccaatg tgtcaaagaa agccagtgcg
     1681 gtcttatagg gcgccggttg cgcctgcggg ttatcgccaa aattcatcaa tacgtccgtc
     1741 tcaggatcat aggccggcca cttcggcaac ccggggccat tcggatcgcc tgtcttcacg
     1801 aagtttgtcc acattgatga aaccgtgtca cctaacttgt aatcgacagg tcgccacgcc
     1861 cagtcttcca gcggaaaggc atcgaagacg taaacgattt cgctgccatg gtacgcaccc
     1921 cactctttcg cgggcctgcc accccataca agcccttccg ggaatggcga gaggtgagtg
     1981 aaaatgtacc aatacgcctt agatttacct gtctttacct ggagcctagc gagcatacga
     2041 gcatttgctt cagctcgatt cgtgcggaac gccaaggccg atttggtagc ctgttcgtcg
     2101 gtatctgctg gaaagacttt gagatactgg tcggctagat ctccgaatgt tttttgggca
     2161 tatgcgggca cgtcggcggc ttttgcggga cccggtgcag ggcctggtgc gtcacttcca
     2221 acgctgccga caatcacagg cacgtcgttt tgtttcccct ctgtgaacgt cgaatacaca
     2281 tcctgtggta gaacccagcc atcaacgatc aaactcatcg gtgctctgga agccttcagg
     2341 atctgttcag caggaagctt gcgtaaatct gcgatggaat gcgcacctag cgactctgcg
     2401 aacttctctc cattcttttc cgcagcagac agaggtacgc ctttgcccaa attttctccg
     2461 ctctgcatga gcgcgcgttc aaaaagcccc ttagcgagcg ggcttgcgag tagagaactg
     2521 acatttgagg ctcctgcgga atggccaaag atcatcaccg tactgggatc gccgccaaac
     2581 ctcgaaatgt ttctctggat ccattgaaga gcagcgatct gatccaagag ggcatagttg
     2641 cctgacgagt gatgctcgga ttccgccgtc aggtcggggt gagctaagaa tccgaagacg
     2701 tttaagcggt aattaaagct gacaaacaca acacccttct ttgcaacgta tgtaccgtca
     2761 tactggtttt ccgatgccgc accgcgggta ttgccgccac catatatgaa caccataacc
     2821 gggagtcggt cagacgcagt ctttgctggc gtccatacgt tgatatacag acaatcctcg
     2881 ctcacaggaa ccaggccttc cctccatgca ttgccctccg tgtcggtcgg catttgcata
     2941 caaacaggcg agaaatgatc ggccctcttt acgccctgcc acggaacgac cggttgcggg
     3001 gggtgccagc ggagttcacc gacgggcgga gccgcataag gaattccccg gtacaagcgc
     3061 acaccgtatg tccactgcgg cgtcggcgtg ccggtgatga gccctccatc cacctgaatc
     3121 ggctcctgca gaccctccga aatcgacaca ggagtggcac ccttggtcgt gcggctaggt
     3181 accgtgagga agagaccgac cacaacggcg cttagaaagg ctaggaccac cggcatacgc
     3241 ttcatctcac acctcgtttc aaagccaatt caccttcaga cgagtcttga ccgccgagca
     3301 acttagtccg catcagaagc cgcgccgcct tgcgagtcca tctattgtgc tttttgcgcg
     3361 taacgatagt tgttgccctg caacatcgtg aggccgttca cttggccatc agctcccctc
     3421 gtgaaagtaa tgagcagata tcccgccaag tgtgcattta taaacatgtc cttattgaca
     3481 ggcactaaag gcatagccgg aagctggaaa ccaggcggcg gccctgttgg cgcggtagct
     3541 tgcggggttg ggccggcagc tccgcggcct cgaccggcag gaccgcccct gccgccgcgc
     3601 ccaagaccgc cgaatccacc gtgaacttgc gccgtaagcg agttcccact cacttgaata
     3661 tccacactgg agttttcgtt cacttggtaa tgcccggcat atgccggcaa gtcggacgca
     3721 ttgaggtgac tgtatggagt cagagccgtg taggagaggt ccggttctct tggcgcgagc
     3781 gggcgaatcc aaagattgcg gtacctcacc ggctcaccgt gatcttgcag gcttaagggg
     3841 gctgcttccg ggccgactac atacggcgct cgtggctccc cggtcgggcc cgtcacggta
     3901 acgtcatcct gaacgtacac gccgttttgg ataatcgtga tatgtgccgg agtgatgaga
     3961 cgtccggttg aatcgaaccg cgggcggcga aagaggatgt ccatcgtctg ccactcgccg
     4021 ggattgcgcg ccgcaacaac gaggggcgga tactgtccat agaccgatgc tgctattccg
     4081 tccgcatagg ttttgttatt tatgtcatcg agaatctgta tctcgtaaac tcccatgatg
     4141 atgacgccgc tattgccctt gttctgtccg tctcccatgg ccggaagagg ttcggcccat
     4201 tccacatgga tctgggcatc tccaaagctt tgcttggtcg tgattgagcc agtacgcggt
     4261 ttaacgatca aggcaccgtc cttgatatcc cactccggag cgctgccacc acgaccaacc
     4321 catgcattca gattggagcc gtcaaagaga acaacggcgt ctgatggaac tcccgctgag
     4381 gagaacgtaa tcgcaggagg catgggccga tccatatcat tcggcaacca cttgtcgtta
     4441 ggcgactgcg ccattgcact gaaaccaaaa agaaacacaa gcacaccaag agcacttatc
     4501 ttcaggcgca tgatcctctc ctcaacattt tgccgttgtc gcgaaagcga cacgaccaat
     4561 tgaccgtctt atttttgcta cacagctaga actcttcacc gaccgagtct cggaactgga
     4621 cgctttagtt ttaggccttg ttcaactttt tgcccgccca gacctgactc catgacttgc
     4681 gaaactccgg agtcacatat cgattcgcct cttcgctatt cgtaaagcga agctttgtgc
     4741 tgtcccactg cagcttgcca tctacgcgca tcgcaatcgt tgaaagcagg atccattcgg
     4801 tgaacggtcc cgcaacactg aaatccgagc aagcccgttc gccgccttta caggcacgaa
     4861 gccagtcaag gtgatgctcg ggtgaacgag tgaggacctc cggtgggagc ttgtaggctt
     4921 tgtgtctctc caacggcaat agccgcacct tagcaccgta tgtgtccgtc gtaaggtatc
     4981 ctttctcacc gacgaaaaca gcaccgtttg cctcctgcat gcctgcgggg attgcgccgc
     5041 cacgtccgcc gcgagcgggc cctgctggcg gattcccgcc gttcgctgct gctgcgttaa
     5101 aggcggccga gaatggattc cggggagtag gtgcgccgaa ggcgtcctct ccgccgatca
     5161 atggctcgcc ttctggaaga tctttgggtt tgtaagcagc tcccctctgc gcgtcgtacc
     5221 agtaaagagt gagcggcggc atgcttccgc gcgaggggaa ctcaaaccga gtgacggtgc
     5281 tattcggata actgagcgtg ttcttccctt cctgctttat cagttcaacg ctcgtcgggt
     5341 ttcccaatag tagagccata ttcggggcac ccaggatgtg caaggccatg tcaccgatgg
     5401 gaccagagcc gaaatccagg aacccgcgcc aattgaaagg ctgatagaaa ccaaactgcg
     5461 tacgatgttg ctggcggtag gtagcaccgc ccgaagtata aggccgcatc gctgccacgc
     5521 caatccaagt atcccaatcg agcgtgtctg gggttttttc ttcatcggga atttcggtca
     5581 tcccctgtgg ccagatcgga cggttggtcc acgcatgcac ttccgttacg ttgccgatct
     5641 cccccgacca aatgatttca gccgcgacgc gcgcgccatc atttgaatag ccttgattgc
     5701 ccatctgagt ggccacgttg tagcgcgcgg ccgcttccgt gagcatgcgg gcttcccagg
     5761 gagtgcgggt cagtggcttt tcgacatata cgtgcttccc tcgttccatc gcccacatcg
     5821 ctgcggtggc gtgcatatga tcgggaatgg cgatcgtgac tgcgtctatg tttttttctt
     5881 ctttatcgaa catcttgcgg aaatcggtgt acttgggaac gttcggatac tgctgaaaag
     5941 tttggccgcc ccgcacggaa tccggatcgg cgaacgccac gaggttttcg cccgagacgc
     6001 ctccaatatc ggccctggct tgcccccctg ctccgatacc tgcaatattt aacttttcgt
     6061 tgggcgactt gtagcccaac attttgagcg aaggaacact accccaacct gcagcaggga
     6121 tagcgcccgc gagcagcgag cctatgaaaa aatgccgcct tgtaattcca tcttttgcca
     6181 tgccgatctt ctccttgagc gcaattctgc tctgccgggc tagaacaaaa acttcaatgc
     6241 aaattggatt tggcgtgggt tccaagccgc gttcattgac gagatttgac cggtcgtcgc
     6301 gtgagaacca gtgagatcca cggcgccgtt cttgaatgca atgctggttc ccgagggccc
     6361 gttgaaattt ggcgtattga acaggttgaa aatttcagtc cggaattgca tcttaactct
     6421 ctcggtcagg gcaaagtcct tgaaaagcga tgcatcgaag cgcgtcagag aaggaccttt
     6481 gaactgacca ggatgcatgt ccccttccgt gccggccact ggctgcacga agcagttgta
     6541 gttgaaccag tgctggatgg ttgggttcgc caatttgccc gagcagatct gatcgggata
     6601 ttggctgcca gcaatttggg aattgctgac ggaatctgtt accgagaagg gaatgcccgt
     6661 ttgccaggat ccggagaagt ttgtcgacca tccctttatg aaaccggcct taactccagt
     6721 gtccgacttg ccgaagggca attggtaatt cagtccccaa gtaaaccgat cgaagacgtt
     6781 catcgaatcg tttccccaat ccgctttttg ccagccccaa gtggcgtggc atactgtcgg
     6841 ctctgctccg caagcacctg gagaagtagc agacgccaaa gaaggacctg cgccatttga
     6901 caggtcgtac cagcatggtt caggcgtagc gaacatcgaa acgacgcaag cccggttgcc
     6961 ggcaatgttg tctaacatat gggaccagac gtagctgaaa ttgacggtca ggccatggtt
     7021 aaagcgtctc tgaaaagaag actgcaagcc gttgtaaaac gaggtgccaa tctgggcggg
     7081 ttcgctcacg cttactgtct tgaggtaggg atagccttgc agaacgccga acccttcatt
     7141 actcataccg ccaaccgtaa gtggcagacc tgttatgttt tcagtgggat tcgctgccaa
     7201 attttggttc tcgccggtat cgaaggcctc gatatgacgt ccgatttgac cgacgtagcc
     7261 cacacttacg acattcgccc cgaattgttt ttgtatcgca agattgaact gttcaagata
     7321 gacctgcttg aagtcggttg tttgaatcgc ctgaatgccc gttccggcgt aattagcggg
     7381 atttgtagcc agcgtaacgt cgaagtaagg agagggaagg ccggccgcca ttaatgcacc
     7441 accggtcttg cctacgttgc taccaggatt cgaggtaggc aggccataag tgacagtgag
     7501 accgttgctg aattgtccaa ctgcgccact gccgaactga gcggtagcac aggatacatt
     7561 cgtcccgact tcgttttgcg cctggcagct tccgcggaat gagaatggag cgttcttctc
     7621 gtagtaaggt gactcatagt tgactggaaa gaaactcaga ccaaaaccgc cgcgaattac
     7681 cgtattgtgg gcgagcgtcg ccgcaaaccc aatacggggc gcaagggcgt tgtaacgcgt
     7741 cggaacgttg gccgtcggac tggagtgttg agggcccggc aagcttggag aaacaagtaa
     7801 gccgatgtac ggattatagt ttgagatctg accattggcc tcggtgaatg gggtaaagat
     7861 gtcgtatcgc actccaaggt tcagcgtgag ccaacgccgg aagcgccaat cgtcttgcac
     7921 ataaacgctc ggctcccaca tccggaactg ctgctttacc aacgccacag ggtggttgag
     7981 cgagacgtcc agaccctcga gcaggtcgcc cggagcgacg cccgtgtacg cgccggtgaa
     8041 agacatcgat ggcgttgatg cctggtcaat ggccgcgcga cggcgaatca ggctggcgcc
     8101 gaatcgcacg ctatgcgcat tctttgtcca agtgagcccg ccgaagtagt gaaagtcggt
     8161 gtcccattcc tccaaaggaa tggcgctgag gtcctgaatg gttgtatagc cgcctgcgcc
     8221 gtttgcgccc gagatcgagg ccgacatgat aatgaagttg ggattcggcc ttccaagcaa
     8281 tgacgaggga gaaccgatgc atgcggtggc gttacacgga aagcccaatt cgttcatgat
     8341 cggttttccc tggttgggtg tataggagta aatactattc tggaagatcg agaacttcag
     8401 attcagaacg agattggaat tgaaaatgtg cacgaagccg ccagatccct ggtcctgagc
     8461 ttcgctgttg gttcctgaaa atgttgtgac cgtcggattg atcagaacac ctccggaagt
     8521 tcccgccgac accgcggcgc cggtcgcagg gttgatcgtc actgccggaa aaccattgcc
     8581 gatcagcgtg ctttcgccat tgtgcgtata tcggaggtac atcgtgtttt tatctgagaa
     8641 gtgctggtcc acttttacgt cccacgtctg gctgttctgt gtgttctccg tagtcgaggt
     8701 gtagttctgt agaatggcac ccgcagcacc ggtattcggc agaggataca tattgaaata
     8761 ggcaagccca agaggagaca aacacgtgcc cggcgcggag cttcccgtgc agagcggcag
     8821 accggagaga tttggccctg gcgtattcgg ctgcgtcgtg gaaccacccg gggagctcac
     8881 gagcttaatg tccgaaaagt ctcccggcgc tgtgcctccg tctgggcacg ccttggcgaa
     8941 aggactgctg ctgttaccga gtcccctttc acaatacgtc ggcacattaa acagagccgc
     9001 ggatttgccg gtcgcgtatc tcaaaccttc gtaatcgccg aagaagaacg tcttgttttt
     9061 cttgatggga ccaccgagag tgccgccata ctggttctga cgaaatgcgg gatttggagc
     9121 cgcgggggat ttcgaacccg ggttaagcgc ctgagcgaag ttgtagttgg gattcgcgtt
     9181 caggacctta ttgcggaaga actcgtacac cgtgccgtgc aagtcatttg ttcccgattt
     9241 cgtaatgata tcgatgaccg cgccgccagt cctgcccaca gacgcatcgt agttgttcgt
     9301 ctgtacgttt acctcttgaa tggcgtcgac ggatgggcgg acgccgattg atccgataaa
     9361 ccgctcattg ttgtccatgc catcgatcaa attgttgttc tgcgtgtcct gttgaccgtt
     9421 cactgatatc tgggagttct ggcggcgatc atcgggacgg ttgccagcga caatcgaccc
     9481 gggtgagcct tcgtttgcac cggccgtccg tgtcaccagc gtgatgatgt tgcgtccgtt
     9541 gagcggcaaa tcttccactt gctgggatgg caccaaaaca ctcacgtttg aagtgtccgt
     9601 ttgcaaagcg ggagctgcgg cctgaacctc taccgtcgtt gtttgggaac cgacgatcat
     9661 tttcgcgtcc acacggacgc gatcccctgc cgaaagcgga atactcggag caacgaacgt
     9721 ctggaaccct gtcgcttcaa ctttgaccac gtatgtcccg acctgcagca atggaaaggt
     9781 gtagttccca tcggaacctg tttgcgccga ttgcgagatg tttgtaccgg tatttgtaag
     9841 cgttacgtgt gctccgggca cgacggcgcc ggtcgcatc
//
LOCUS       DATNDT010000182         2070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3850106, whole genome shotgun sequence.
ACCESSION   DATNDT010000182 DATNDT010000000
VERSION     DATNDT010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3850106"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="VN788_05950"
     CDS             <1..396
                     /locus_tag="VN788_05950"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011824553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HXU20108.1"
                     /translation="GEDARRALDEGAEGVIVSNHGGRQLDGVAASLRALPEVVKAVGG
                     RAEVLMDGGVRRGSDIAKAICLGARAVLIGRAYAYGLGAAGEAGVARAIAILRADLER
                     TLALLGCESIRELNRSYVDVPASWQRQLP"
     gene            complement(393..1847)
                     /locus_tag="VN788_05955"
     CDS             complement(393..1847)
                     /locus_tag="VN788_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HXU20109.1"
                     /translation="MRTENKAPLTSGGHYNRPTRERIESAVSDLKSLLGARVTVAESI
                     REHHSHGESYHPAALPDAVCFPHSTDEVSKILKISAKYQVPVIPFGAGTSMEAQVNAI
                     YGGITIDMREMNRVIRISPEDLQATVEAGMPRKQLNKALENTGFTFFIDPGADATIGG
                     MTSTRASGTTAVRYGTMRENVMALTVVLADGRIIKTGTAARKSAAGYDLTRLFVGAEG
                     TLGVITEVTLRLHPLPEAVAAACCTFNTIRDAVETAIETIQLGVAVARIELMDEAQID
                     ACNRYSKTQYPVAPLLFFEFHGDSERNVNDQAETVRNLAKEHNGRDFEWFTTPEERET
                     LWKMRHEAYFATLAIRPGCKMFTTDICVPISRLADCIVESKKDAESASFPTTVVGHVG
                     DGNFHMAFILDPDNPAELAEAKKLEEKIILRALEMGGTCTGEHGIGTGRAKYLSTEHG
                     AALDVMRAIKQALDPDNRMNPGKMVEPQALLPQS"
     gene            complement(1876..>2070)
                     /locus_tag="VN788_05960"
     CDS             complement(1876..>2070)
                     /locus_tag="VN788_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20110.1"
                     /translation="RVDPGALSRGKRTTHPTPELEREAHDILGRTVVDLRDRTFQVVR
                     IEPTLAGDGSVLYYDVIVHL"
BASE COUNT          394 a          580 c          609 g          487 t
ORIGIN      
        1 ggcgaagatg cgaggcgtgc gcttgatgag ggagcggaag gcgtgatcgt atcgaatcac
       61 ggcgggcgtc agctcgatgg cgtagccgca tcgctgcggg cgctgccgga ggttgtgaag
      121 gcggtcggcg ggcgcgctga ggttttgatg gatgggggag tgcggcgcgg gagcgacatc
      181 gcaaaagcaa tttgcctggg ggctcgcgcg gtgctgattg gacgcgcgta tgcatacggc
      241 cttggcgcgg ccggcgaggc gggcgtggcg agagctatcg cgattctgcg cgccgacctg
      301 gagcgtacgt tggcgctgct tggttgcgaa tcgattcgag agctgaatcg gtcctatgtg
      361 gatgttcccg caagctggca gcgacagctc ccctaggact gcggcaggag cgcctgcggt
      421 tcaaccatct tgcctgggtt catcctatta tctggatcca atgcctgttt aatggcgcgc
      481 atgacgtcga gcgctgcgcc gtgttccgtt gacaggtatt ttgcccttcc cgtgccaatc
      541 ccgtgctcgc cggtacaagt cccgcccatt tcgagcgcgc gcagaataat tttctcttcc
      601 agtttcttcg cttcggcaag ctctgccgga ttgtccggat cgaggatgaa agccatgtga
      661 aagttgccgt cgcccacatg tccgacaacc gttgttggaa aagatgcgct ctcggcgtcc
      721 ttcttcgatt cgacgatgca atcggccagc cgcgaaatag ggacgcagat gtcggtggtg
      781 aacattttgc agcccggacg aatcgccagg gttgcaaagt aggcttcatg gcgcattttc
      841 caaagcgttt cgcgttcctc tggcgtcgta aaccattcga aatcgcggcc attgtgttct
      901 ttcgcgaggt tgcgcaccgt ctctgcttga tcgttcacat tccgctcgct gtctccatga
      961 aattcgaaaa ataaaagggg cgcgaccgga tattgtgttt tggaataacg attgcacgcg
     1021 tcgatttggg cctcgtccat cagctcgatt cgtgccactg ccacgccgag ttgaatggtt
     1081 tcaatcgccg tctcaacggc atctcgaatc gtgttaaaag tgcagcaggc cgccgcaact
     1141 gcctcgggca atggatgcag gcgaagtgtg acttccgtaa tcacgccgag cgtcccctca
     1201 gcgcccacaa aaagacgagt cagatcgtaa cctgccgcgg attttcgcgc ggctgtgccc
     1261 gttttgatga tgcgtccgtc cgcgagtacc acggtcagag ccattacatt ttcccgcatg
     1321 gtgccgtaac ggaccgccgt tgtgcccgaa gcgcgcgttg acgtcatccc gccgatcgtc
     1381 gcgtcggctc cgggatcgat aaagaatgta aagccggtgt tctccagagc tttattaagc
     1441 tgtttgcgcg gcattcccgc ttccaccgtc gcctgcaaat cctcaggact gattcggatg
     1501 actctgttca tttcacgcat gtcaattgtg atgccgccat aaatcgcgtt cacctgcgct
     1561 tccatcgaag tgcccgctcc gaagggaatc accggcactt gatatttcgc gctgatcttg
     1621 aggattttgc tgacctcgtc ggtagagtgt ggaaagcaga cggcatccgg gagcgcagcg
     1681 ggatgataag actcaccatg gctatgatgt tcacgaatcg attccgctac tgttactctc
     1741 gcgccgagca gagatttcag atcgctaacg gcgctttcga tgcgttcgcg tgttggacga
     1801 ttgtagtgtc cgccgctggt caatggcgcc ttattttctg ttctcatgtt cgccgaatac
     1861 aacacaatcg acttgctaaa gatggacaat cacgtcgtag taaagaaccg atccgtcccc
     1921 cgcaagggta ggttcaattc gcaccacttg gaaggttcga tctcttagat cgaccaccgt
     1981 ccgtcccaat atgtcgtgcg cctcgcgctc cagctccgga gtgggatgcg tagtgcgctt
     2041 cccccgtgaa agagccccgg gatcgacccg
//
LOCUS       DATNDT010000183        14627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2763936, whole genome shotgun sequence.
ACCESSION   DATNDT010000183 DATNDT010000000
VERSION     DATNDT010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 14627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14627
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2763936"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..3814)
                     /locus_tag="VN788_05965"
     CDS             complement(23..3814)
                     /locus_tag="VN788_05965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU20111.1"
                     /translation="MVVEVHTCVRKLVFVFILTLGFFVFTSQAQTSGQGGLEGTITDS
                     TGAVIPSAAITATNQASGVSTSRQSSSAGAYEIAPLIPGVYTVTVNAKGFQTIRQENI
                     EVNGLTVTGYNAVMTVGRETQTVVVTTAPPQLQTTNATLGGVITNQTYESLPNLMGGQ
                     QRDPTAFATLAPGAQSGARAPVMAGTGSYLSEVYLNGIPTTTANMQSDNRLIVNGVPV
                     ESVDQMQVMSSSPTAEYQGAGAISFTTKSGGDQYHGTVADFVRNTMFDTWGFTAPYAT
                     KRVLVNGVLTTVPAGKPVEHQNELSASLGGPIPWTRHKGFFFVNWDQYHGRNGVSPSL
                     FTVPTALMRQGNFSELGTGTYIYNPLTNSCTAGVCTRQPFAGNIIPSDYISSISLYEQ
                     KFLPPPSLPGISNNYLAGGLSGFDNHEFVFKIDYNLTSKQRYSVVASHGVRQSVGYGA
                     QLPLPYTVGDSSVISPTTLIFEHTYVLTPSVVNQFNYGFTRFPQPVQAPTDNLKPYRG
                     GPDVGIGGLPPGQSSGNFPGSSFATSKAFPTAPSQWTENGAADASHNVVPNAFTIVDN
                     LQWTKGKHSITFGLQTQWLEDNVTSQTSPSGIYTQSWNPISTANYNGGANLDSTTTGY
                     SYASFLLGAVNSAGTSVPLFTETAGRYHTWSPYVQDDWKLSPKLTINLGLRWDYLPPY
                     HEDQDRFSFFNPTAINPLTGTPGELQFAGNRGADISCQCKTPVHTYWNNWGPRVGFAW
                     SPIAKTVIRGGFAMSYSRAGGVGGRAGDSTGTGQTGFNSSIILPPAVNTSANAGPSFY
                     LNDSPAFQAAGLANTNFGGPGYSIPAPAGPSAAALTIGTGNYVNSSGKYVSPGGTAAY
                     ADPYLSGRAPEFEFYNFGVQRAVTNALTLTANYAGSESHFVAGAGEPGFWSGQLDPAI
                     VAALGSTLATDNTTNILNAPATPANIAIAEAADPNITIPAFYAAAGAVSNSPTIGRAL
                     RPFPQYSNPPSVEWDNIANISYNALQISLQQRPWKGLSYTLNYTYSKDIGDDGAVRSA
                     FPVPAADSSNGKPIPGNNRSGRDLTSLDMPHSLNIYGLDELPFGKGHWGGNNFFVRNI
                     VGGWAISGIYTYYSGGPLFITGSGCTAPSQGTCMPDVVPGVNIRQNGNWGDGVTGNNL
                     AAISYLNSAAFTLPNAFPLPANATSKAVPITKIGDAPRSSLNVWGPNRFNLDLSIRRT
                     FNITERVKFLFQADCSNVTNSVTFGGINTTWSPSASSSFGKVTSARGNRDFQFSGRIT
                     F"
     gene            4128..4766
                     /locus_tag="VN788_05970"
     CDS             4128..4766
                     /locus_tag="VN788_05970"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HXU20112.1"
                     /translation="MRLLCINSTPMSDEAISRRLTKAFVQGWRCKNRDGIVIARDLAS
                     IDIPAINAEWICANYTPEGSRTKQQDELLRLSKELIAELHDADEYVIGMPMHNWGPPS
                     KFKLWVDQIVVPGCRGVLSQKSCTFVIATGWHHRPNSARASENYLVPWLRTLFGYLGV
                     EDVRFIMADGSMEIAKGGIDREAFLAPHIEAIHGLFMEGRDDYLAARYMEGV"
     gene            complement(4940..5431)
                     /locus_tag="VN788_05975"
     CDS             complement(4940..5431)
                     /locus_tag="VN788_05975"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU20113.1"
                     /translation="MGAAPIWDGQPAVVSPLRNPRPYTEITEIVPARTPKPGPRRYFT
                     RVPTHAIGVASERRRYPRAALSLPLRLVRVEGAVDFHPVLLVTRNISSSGVFFLAPRE
                     IHLGTAIELEVALVARPFGSGSVQMITAAHVVRTEPCDTPGWHGYGASFDDFALQRDD
                     FIP"
     gene            5685..6800
                     /gene="ada"
                     /locus_tag="VN788_05980"
     CDS             5685..6800
                     /gene="ada"
                     /locus_tag="VN788_05980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012411506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HXU20114.1"
                     /translation="MQLNILDTVGYVGAPAALWEAVEKRDSRADGKFVYGVRSTGIYC
                     RPSCASRRPSRDKVEFFALPEAAERQGFRPCKRCLPRNAEVTDGRLRAVSRVCRAIEQ
                     QIVNDGTDEGALRLPKLAGGVKMSTDQLERAFRRTIGITPRQYADAVRMKRLKSQLRK
                     GGNVTTALYDAGFGSSSRLYERAPSQLGMTPRAYGHGGEGMQIHYTVVGSPLGRLLVG
                     ATEKGISAVYLGKSDKELEDALREEYPRAEISEDAQARAELRTWVEKIVAHLKGKEPH
                     LDLPTDVQGTAFRRRVWEELRKIPYGKTMTYTDVAKRIGKPKAIRAVARACATNPVSV
                     VVPCHRVIRQDGSLAGYRWGLERKQALLEKETEMAKD"
     gene            6867..7253
                     /gene="panD"
                     /locus_tag="VN788_05985"
     CDS             6867..7253
                     /gene="panD"
                     /locus_tag="VN788_05985"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HXU20115.1"
                     /translation="MLTSFCVAKIHRATVTEADLNYVGSITIDEVLLEKSGIKPFQMV
                     KVHNLSNATAWQTYAIAGARGSGTICLNGPPARLFQPGDKVIIIAEAYLEPAEVDDLV
                     PVVVFVDEKNRVTKVEKHALRATSPA"
     gene            complement(7255..7974)
                     /gene="rlmB"
                     /locus_tag="VN788_05990"
     CDS             complement(7255..7974)
                     /gene="rlmB"
                     /locus_tag="VN788_05990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HXU20116.1"
                     /translation="MNWMTGFHAVEEALAAGRPLDRIVIARGRHGERMEAIVRQAKSR
                     GVPVRFEDRQQIDRLAGTREHQGLAALAAAKKSVEIEDLLAANDGHGLLVLLDGVEDP
                     HNLGAIIRSSLAAGAQGVVIPERRAAGLTDTVERASAGALAHLPIARVKNLVRAMEQM
                     KEAGYWLIGLDERAEKKYTEVDLKGSVGIVLGSEGEGLHDLTRKRCDFLVSIPTTGPV
                     RSLNVSVAAGIILFEAIRQRQ"
     gene            complement(8067..8543)
                     /gene="ispF"
                     /locus_tag="VN788_05995"
     CDS             complement(8067..8543)
                     /gene="ispF"
                     /locus_tag="VN788_05995"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HXU20117.1"
                     /translation="MNRSGIGYDLHRLSEGRKLVLAGVEIPFDKGPVGHSDGDVLCHA
                     LCDAILGAGGLGDIGTHFPDTDPKWKGAASLQFIEHACKLLASRRMRVVHVDVVVICE
                     RPKLGPHFPAIRSALVEVLKIPGEDISLKAKTNEGVDAVGRGEAIAAHAIATVSIH"
     gene            complement(8540..9256)
                     /gene="ispD"
                     /locus_tag="VN788_06000"
     CDS             complement(8540..9256)
                     /gene="ispD"
                     /locus_tag="VN788_06000"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HXU20118.1"
                     /translation="MNRVTAIIPAAGLGTRMGADQPKQFIEIDGVPLVIFTLRRLAAC
                     SAITDFILSTRAEEIVSLQDKVAKASLGRPARVIHGGDTRQQSVANAIAQVDPATEIV
                     LVHDAVRPFITAEQTARVIAEARLRGAAILAIPAIDTVKEVKRASLPEDVALITATIP
                     RERIVMAQTPQAFSFPLLRDAFRKAQEDGVTGSDEAVLVERLGHEVFAVLGSERNLKI
                     TRPSDLELAHFYLEQERVPK"
     gene            complement(9513..10748)
                     /locus_tag="VN788_06005"
     CDS             complement(9513..10748)
                     /locus_tag="VN788_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXU20119.1"
                     /translation="MQRPSNAPPRTQGSASPETPVSPRPVMLSRASKGVGTFWDTLVI
                     RIIFVVICGVAGFHFRPFSITPVEGTVAGVCFASAIILVEVRLRKVTLRRLIGAAVGS
                     ILGILGAYLTSLIMSHTTMPESTRSFFSLSIFVVSTYVGLMLGANKGDMLNLQAFGGV
                     FGSERGSRHITKLLDTSVIIDGRVADIADALFIDGALVIPQFVLRELQLVADSADPLK
                     RQRGRRGLEVLQRIQKMQHLDVQIAEDDFPHIPDVDLKLIELAKRYDAKIVTNDFNLN
                     KVATLQGIEIMNVNLLANALKPVVLPGETMRVFILREGKEYNQGVAYLDDGTMVVVDG
                     ARKMINKTIDISVTSVHQTTAGKMIFGRYDDRSEQARATAGPGAGPTAVDAPASRPAE
                     AGTPGERSPRALYPEPGRS"
     gene            complement(10951..11607)
                     /locus_tag="VN788_06010"
     CDS             complement(10951..11607)
                     /locus_tag="VN788_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3800 domain-containing protein"
                     /protein_id="HXU20120.1"
                     /translation="MALVIYVDESGDLGWNFSAPYRDGGSSRYLTIASLCVPSDKKHL
                     PKRLIKDLYNRFKWPYRVERKWVDLSEDERTYFAESAHKLCVAHNDIHLHSITVNKQN
                     VMAHIRQDGNKLYNYMIRLSLLNRMATHGVVTMVPDPRSIKVESGNSLHDYLQIELWF
                     RKNAMTNLLTQPVESHNCSNLQFTDMLAGVIQARFEDNDSKNFQIIQPVTTISRLYFG
                     "
     gene            complement(11801..12624)
                     /locus_tag="VN788_06015"
                     /pseudo
     CDS             complement(11801..12624)
                     /locus_tag="VN788_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_040609789.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(13424..13663)
                     /locus_tag="VN788_06020"
     CDS             complement(13424..13663)
                     /locus_tag="VN788_06020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20121.1"
                     /translation="MPKRISKNLKDPNQIAAAVVALSTSEEPQVDRETLSRVMAEMGR
                     KGGKIGGKRSMETMTAARRKKRAKAAAKARWKNKK"
     gene            complement(13855..>14627)
                     /gene="alr"
                     /locus_tag="VN788_06025"
     CDS             complement(13855..>14627)
                     /gene="alr"
                     /locus_tag="VN788_06025"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HXU20122.1"
                     /translation="IPFHLKINTGMNRLGIEATDIDSFAAALAECKHIALEGTFTHFA
                     SAEDFSARQTDDQEQLFRNCLARMRELGVSPGIVHMANSGAICARPTTFADMVRPGAV
                     LYGYYQSFDPPHKGEEVREKLPITPCLSLRARIITLRDVPAGEGVGYSARFVTERPTR
                     IAVINAGYADGILRQRTNRGCVLVRGRRVPLVGTISMDLTTLDVTDVPEAQLGDIVTI
                     YGKDGADSIVVSDVAREIGTVTSDLLCALGRRVPRYYV"
BASE COUNT         3250 a         3724 c         4452 g         3201 t
ORIGIN      
        1 gcgctcccgg ggaaaacatt cgttagaagg tgatcctccc tgagaactgg aagtcgcggt
       61 tgccgcgggc cgaagtaact ttgccgaagc tgctgctggc ggatggcgac caagtggtgt
      121 tgattccacc gaacgtgacc gagttcgtca cgttgctgca atcggcctga aagaggaatt
      181 tgacgcgctc ggttatgttg aaagtacgcc gaatgctgag atcgagatta aagcggtttg
      241 gcccccaaac attgagacta ctgcgcggag catcaccgat tttcgtgatg ggcacggctt
      301 tactcgttgc gttcgcgggt aatggaaagg cgtttggtag agtgaacgcg gcggagttga
      361 gataacttat cgcggcaaga ttattgccgg tgacgccatc cccccagttt ccgttttgcc
      421 gaatgttgac gcccgggacg acgtctggca tgcaagtgcc ttgtgacggc gccgtgcagc
      481 cggaacccgt aatgaagagc gggccgccgg aataataggt gtaaattccg gatatcgccc
      541 agccgccgac aatatttcgc acgaagaagt tgttgccacc ccaatggcct ttgccgaacg
      601 gcaattcatc gagaccgtaa atattgagac tatgcggcat atccagcgag gtcaggtcgc
      661 ggcctgaacg attgttacca ggaatgggct tgccgttgga tgagtccgca gcaggaaccg
      721 ggaaagcgct gcgtacggca ccatcgtcgc caatgtcctt tgagtacgta tagttgagag
      781 tgtagctcag acctttccat ggacgctgtt gcagcgaaat ttgcagcgcg ttgtaagaaa
      841 tgttggcgat gttgtcccac tcgacgctcg gagggttcga gtactgaggg aacggacgca
      901 aagcgcgacc gattgtcgga gagttgctga cggcaccggc ggcggcgtaa aaggccggta
      961 tggtgatgtt cggatcagcc gcttctgcga tggcaatatt cgccggcgta gccggtgcgt
     1021 tgagaatatt tgttgtgttg tctgtagcga gcgtagaacc gagagcggct acgatcgctg
     1081 gatccaactg tcccgaccag aagcctggct caccagctcc ggcaacgaaa tgggattcgc
     1141 taccggcgta gttggccgta agcgtcaatg cgttggtgac cgcacgctgg acgccgaaat
     1201 tgtagaactc gaactcgggc gcgcggccag acaaatacgg atcggcgtag gcagccgtcc
     1261 cacccggaga cacatacttg ccagaactgt ttacataatt tccggtgcca atcgtcaaag
     1321 ctgcggcgct gggtccggcg ggtgcaggaa ttgaatagcc cggaccgcca aaatttgtat
     1381 tggcgagtcc ggctgcctgg aatgcggggc tatcgttcag atagaaggat gggccggcat
     1441 tcgcgcttgt gtttactgcg ggtggcagaa tgatgctcga attgaaacca gtctggccgg
     1501 ttccagtcga atcgcccgca cgacctccga cgccgccggc gcgcgaatag gacattgcaa
     1561 aaccgccgcg aatgactgtc ttggcaattg gggaccacgc gaagccaaca cgaggtcccc
     1621 agttgttcca ataggtgtgg acgggagtct tgcattggca actgatgtcc gcgccgcgat
     1681 ttccagcgaa ttggagctct ccgggcgtgc cagtgagcgg attgattgcg gtcgggttaa
     1741 agaaggagaa ccgatcctga tcttcgtgat agggaggcaa gtaatcccaa cgaaggccga
     1801 gattgatcgt taactttgga ctgagcttcc aatcgtcctg aacgtaggga gaccaagtgt
     1861 gataccggcc ggccgtttcc gtgaagagag gaacggaggt gcctgcgcta ttcacagcac
     1921 ctaacaagaa gcttgcgtag gaatagcctg ttgtcgtgct gtccaaattc gcgccaccat
     1981 tgtaattggc ggtcgaaatg ggattccacg attgagtgta gatgccggaa ggactggttt
     2041 gggaggtgac gttgtcctcg agccactgcg tctgcaatcc aaacgtgatc gaatgctttc
     2101 cctttgtcca ttgcaggttg tcgacgattg taaatgcgtt tggcacgaca ttgtgtgagg
     2161 catcggccgc gccattctcc gtccactgcg acggggcggt ggggaaagcc ttcgatgtgg
     2221 cgaaggaaga tcctgggaag ttgcccgagg attgaccggg agggagaccg ccgattccga
     2281 cgtctggtcc gccgcgataa ggcttcagat tatcagtggg cgcctgcacg ggctgaggaa
     2341 agcgcgtgaa tccatagttg aactggttga cgacactcgg agttagaaca taagtatgtt
     2401 cgaagataag ggttgtgggt gaaatgactg agctatcgcc gacagtgtaa ggcagcggca
     2461 actgtgcgcc atagcccaca ctttgccgga caccgtggga ggcgacaacc gaatagcgct
     2521 gcttgctggt gaggttatag tcgatcttga agacaaactc atggttatca aacccggata
     2581 gaccgcccgc aagatagtta ttcgaaatac cgggaagcga tggcggtggg agaaacttct
     2641 gttcgtaaag cgagatggag gaaatgtagt cagagggaat gatgtttccc gcgaaaggct
     2701 ggcgggtgca aacgccagcc gtacatgaat tcgtaagtgg attatagatg taggtgcccg
     2761 tgccgagttc ggagaagttg ccctggcgca ttagcgccgt gggtactgtg aacaaggaag
     2821 ggctgacgcc attgcggccg tgatattggt cccaattgac aaagaaaaag cctttgtgcc
     2881 tggtccatgg gataggacca ccgagcgacg cagataattc gttttgatgt tcaaccggtt
     2941 tgccggccgg aacggtagtg aggacgccgt ttacaaggac gcgctttgtc gcgtacggag
     3001 ccgtgaaacc ccaggtatcg aacatcgtgt tgcgcacgaa atcggcaacc gtgccgtgat
     3061 attggtcgcc tcccgatttg gtagtgaagc tgatcgcgcc ggcgccttga tattctgctg
     3121 tgggcgaact ggacatcacc tgcatctgat ccactgattc gacgggcacg ccattgacga
     3181 tgaggcggtt atcgctctgc atgttggccg ttgtggtggg aattccattc aaatagacct
     3241 ctgataaata acttcccgtt cccgccatga cgggagcgcg tgcgccggac tgcgcacccg
     3301 gcgcaagtgt agcgaacgcc gtcgggtcgc gctgctggcc tcccataagg ttcggcaaac
     3361 tttcatacgt ttgattcgtg atgacgcctc caagcgttgc gtttgtcgtt tgaagctgcg
     3421 gaggcgcggt ggtaacgacg acggtctgag tttcgcggcc cacggtcatg acggcgttgt
     3481 atccggtaac cgtgaggccg ttgacttcga tattttcttg ccgaattgtt tgaaaacctt
     3541 tggcgttgac cgtgaccgtg taaacaccag gaatcaacgg cgcaatttca taggcgccgg
     3601 cagacgaact ctgacgactg gtagagacgc cgctggcctg attcgtggct gttatggcgg
     3661 cactcgggat gactgcgccg gtagaatcgg tgatggttcc ttcaaggcca ccttggccgc
     3721 tggtttgggc ttgtgatgtg aaaacgaaaa aaccgagtgt cagaataaaa acgaagacaa
     3781 gcttgcggac acaggtgtgc acttcaacta ccatttttcc ccctttagac cgataggggt
     3841 gccatgttgc atggccgcac cctaggacac atgtcgcgac agaacatagt tctgtattga
     3901 cagctgttcg gaaaaatagt tctggggaaa aatggtgtca atctgaaaca tttaaaggat
     3961 acgtttagtt ttgctaaaag accgaagaat cggtgcattt cgccatggcc tgtgcagcgc
     4021 aataggaaat gcaactacac gcgcgaggtt tatttcacaa gcggtcacat tgtcgtgttc
     4081 tcaggcgtca ttgttatgag caacgaaaac tggatcgaga ggcaacaatg agattgctgt
     4141 gcattaactc cacgccgatg agcgacgagg ccatatcgcg aagactgacg aaggcattcg
     4201 tgcaaggctg gcggtgcaaa aatcgagatg ggatcgtaat agcgcgagat ctggcatcta
     4261 tcgacattcc agcgatcaat gcagagtgga tctgcgcaaa ttacacaccg gaaggatcgc
     4321 gaacaaaaca gcaggatgaa ttgctgaggc tttcgaagga actgatcgcg gaattgcatg
     4381 acgccgacga atacgtgatc ggtatgccga tgcacaactg ggggccacct tcgaaattca
     4441 aattgtgggt agatcagatc gttgttccgg gatgccgagg cgttctgagc cagaaatcct
     4501 gcacatttgt catcgcgact ggttggcatc acaggccgaa ttcggcgaga gcatctgaga
     4561 attatctggt tccgtggttg cgaacgttat tcggctatct cggcgtagag gacgtgcgat
     4621 tcatcatggc agatggcagc atggagatcg ccaaaggcgg tattgatcgc gaggcatttc
     4681 tggcgccgca cattgaggcg attcatggat tgtttatgga ggggcgcgac gattatttgg
     4741 ctgcgcgata tatggaagga gtttgagcag tgatcggcta aaccgcccta gggaggcaag
     4801 agaaagccgc tcgctacgtg aagcaacatc aagaccaaag tcaaaagccc acaccgagga
     4861 agagcccgga aaaattggcg gccacaggcg acgctacgaa actgtgcgca taatcgcctg
     4921 agtttcttgc agcaaattgt tagggaatga agtcgtcccg ctggagggcg aagtcgtcga
     4981 agcttgcgcc gtaaccgtgc cagccgggtg tatcgcacgg ctctgtgcga acgacgtgag
     5041 cagcggtgat catctgcacg ctacccgaac cgaatggccg cgcgaccaag gcgacttcaa
     5101 gttcgatggc cgtgccgagg tgaatttcgc gtggagcgag gaagaagaca ccgctcgaac
     5161 tgatgttgcg cgtgacgaga agcaccgggt ggaaatccac ggctccctcg acgcgcacca
     5221 agcggagcgg cagggaaagg gctgcgcgcg gataacggcg gcgctcactg gctactccaa
     5281 tagcatgagt tggaacgcga gtgaaatagc gtcgcggtcc cggcttaggc gttctggccg
     5341 gaacgatttc tgtgatttcg gtatagggcc tcggattgcg cagcggcgag actacggctg
     5401 gctgtccgtc ccaaatcgga gcagctccca tgatactctc ccaatgcttt gcctaccctg
     5461 acccccgtca ggtccaccga atatgccctc gtagacagaa tgcgtcaacg ccgcatacac
     5521 gaaagaatgt agaaggtgtt ctaaaggtta acccttagat agaacctggg tgattgccct
     5581 cagggcacct gttcggccag agcggcgaca ttttgccgcg aaaatcggat agaagggcac
     5641 gtcgagtggg attatcttac tatacgaagc aaggcgggac cgaaatgcag ctaaatattt
     5701 tagatacagt gggatacgtg ggggcgccgg cggcgctgtg ggaggccgtg gaaaagcggg
     5761 actcgcgggc agacggaaag ttcgtctatg gagtccgttc cacggggatt tattgtcggc
     5821 cgtcgtgcgc ttcgcggcgg ccttcgcggg acaaagtcga gttctttgcg cttccggaag
     5881 ctgccgagcg gcaaggcttc cggccatgca agagatgctt gcctcggaac gccgaggtta
     5941 cggatggcag gctgcgggcc gtctcgaggg tgtgccgcgc gattgagcag cagattgtta
     6001 acgatgggac ggatgagggc gcgttgaggc tgccgaagct ggcgggagga gtgaagatga
     6061 gcaccgatca gctcgaacgg gcgttccgca ggacgatcgg aattacgccg aggcaatatg
     6121 cggacgcggt gcggatgaag cgattgaaat cgcaattgcg aaaaggaggc aatgtgacaa
     6181 cggcacttta cgatgcggga tttgggtcat cgagcaggct ttacgagcgg gctccgagcc
     6241 aactgggaat gactccgcga gcgtatgggc acggcggaga agggatgcag attcattaca
     6301 cggttgtggg ttctccgctg gggcggctgt tggtgggcgc gacggagaag gggatcagcg
     6361 ccgtctactt ggggaaatcg gacaaagagc ttgaggacgc gttgcgagag gaatatccgc
     6421 gcgcggaaat cagcgaggat gcgcaggcgc gcgcggagct tcgaacgtgg gtggagaaaa
     6481 ttgtggcgca tttgaaggga aaggagccgc atctcgatct gcctacggat gtgcagggga
     6541 cggcgtttcg caggcgggtg tgggaggaat tgcggaagat tccgtatggg aagaccatga
     6601 cgtacaccga cgtggcgaag aggatcggga agccgaaggc gattcgggca gtggcgcgcg
     6661 cttgtgcgac gaatccggtt tcggtggtgg tgccttgcca tcgagtgatt cggcaggatg
     6721 gaagtttggc ggggtaccgg tggggattgg aacggaagca ggcgctattg gaaaaggaaa
     6781 cggagatggc gaaggattag ggcgcgcgtg gtcacaggca tcggggcgag cgggtggtta
     6841 cgtgggaatt tgtgtaaaat ggaagcatgc tgactagctt ttgtgtggcg aagatccatc
     6901 gggcgaccgt cacggaggcc gacctgaatt atgtcgggtc gattacgatt gacgaagtgc
     6961 tgcttgagaa gagcggaatc aaaccgttcc agatggtgaa agtgcacaac ctttcgaatg
     7021 ccacggcatg gcaaacctat gccatcgccg gggcgcgggg cagcgggacg atctgtctga
     7081 atgggcctcc ggcgcggctg tttcaaccgg gcgacaaggt gatcattatc gccgaggcgt
     7141 atttggaacc tgccgaggtg gatgatctcg tgccggtggt tgtctttgtc gacgagaaga
     7201 accgcgtgac gaaagtcgag aagcacgcgt tgcgcgcgac gagtccggcg taagttattg
     7261 gcgctggcgg attgcttcga acaagatgat gccggcggcg acggatacgt tgagggagcg
     7321 aacgggcccg gtggtgggaa tcgaaacgag gaaatcgcag cgtttgcgcg tgagatcgtg
     7381 aaggccttcg ccttcactgc ccaggacgat gcctacggaa cctttcaggt cgacttccgt
     7441 gtatttcttt tccgcgcgtt catcgagccc gatcagccag tagcccgcct ctttcatctg
     7501 ctccatcgcg cggacgagat ttttcacacg agcaatcggc aaatgggcga gtgctccggc
     7561 tgacgcgcgt tcgacggtgt ccgtcaatcc tgcggcgcgg cgctctggaa tgaccacgcc
     7621 ttgcgcgcct gcagcgagcg agctgcggat aatcgcgccg aggttgtgcg ggtcctccac
     7681 gccatcaaga agaactaaca agccgtgacc gtcgttggct gcgagcaggt cttcgatctc
     7741 taccgatttc tttgctgccg cgagcgcagc taagccttga tgttcgcgcg tgcctgcgag
     7801 gcgatcgatc tgctggcgat cctcgaagcg gaccgggacg ccacgcgatt tcgcctgccg
     7861 gacgatggct tccatgcgct cgccatggcg accgcgggcg atgacaatgc gatcaagcgg
     7921 acggcccgcg gcgagagctt cttcgacggc atgaaatcca gtcatccagt tcatgatttc
     7981 accggtgtag tccagtgtgg cgcatcagta gcggaccgat ggggcagcaa gcatgcgccc
     8041 gtacggaaat aaatcgatat atcgcactag tggatgctga cggtggcgat ggcgtgggcg
     8101 gctatggctt cgcctcggcc tacggcgtct acgccttcgt ttgttttggc ttttaggctg
     8161 atatcttcgc ccggaatctt gagaacctcg accagtgcgg agcgaatcgc gggaaagtgc
     8221 gggccgagtt ttgggcgctc gcaaatgacc acaacgtcca cgtgaactac gcgcatgcga
     8281 cggctcgcaa gcagcttgca ggcatgctcg ataaattgca ggcttgccgc gcctttccat
     8341 ttcggatcgg tgtctggaaa atgcgttccg atgtcgccca atccgccggc gccaaggatt
     8401 gcatcgcaga gagcatggca gaggacatcg ccgtccgaat gaccgaccgg gcccttatcg
     8461 aatggaattt cgactcccgc gagaactaat tttcggcctt cggacagacg atgaagatcg
     8521 tacccgattc ccgagcggtt catttcggga cgcgctcctg ctcgagatag aaatgcgcca
     8581 actcgaggtc agatggccgc gttatcttca aattgcgctc cgaacctaat accgcaaata
     8641 cttcatggcc caggcgttcg acgaggacgg cttcgtcaga gccggtcacg ccatcctcct
     8701 gcgctttgcg gaaggcgtcg cggagcagcg gaaacgagaa cgcctgcgga gtttgagcca
     8761 ttacgatccg ttcgcgagga atcgtcgcgg taataagcgc aacatcttcg ggaaggcttg
     8821 cacgctttac ttctttcacc gtgtcgatgg ccggaatcgc caaaattgcg gctccgcgca
     8881 aacgtgcttc ggcgatcacg cgagcggtct gctccgccgt aataaagggc cgcacagcat
     8941 catgcacaag cacgatttcc gttgcggggt cgacttgggc gattgcgttc gccacggatt
     9001 gctgacgcgt atcgccgccg tggatgacgc gtgcgggccg cccgagactt gctttggcta
     9061 ctttatcctg cagcgaaacg atctcctcgg cgcgcgtgga gaggatgaaa tccgtaattg
     9121 ccgagcatgc agcgaggcgg cgcaacgtaa aaatcacgag cggaacgcca tcgatctcga
     9181 tgaattgttt gggctggtcc gcgcccatgc gcgttccaag cccggcggcc ggaatgatgg
     9241 cagtcacgcg attcataggt tggcagttta gcgcagttcg cgcgattcga ggagcaaact
     9301 aacgcggaag agagatagga aaagcccgag agagccttcg gcatgccgga acagccgacg
     9361 cgcctcagaa tttgcgacta aattcgcagg agagcgaagc aacagggatc cttcgccgcg
     9421 cccttaaaga cgggcgtggc agacaagcct cagggtgacg atcttcgttt actaagaacg
     9481 cgactcttat caacgctacg taagcggctg ggtcacgagc ggccgggttc gggatagagg
     9541 gcacgcggcg agcgttcacc gggtgttccg gcttctgccg gacggctcgc cggagcatcg
     9601 acagcggtag gtccggcgcc aggaccggcg gttgcacggg cctgttcgga tcgatcatcg
     9661 tagcggccga aaatcatctt gcctgcggtt gtctgatgga ccgatgtaac gctgatatcg
     9721 atcgttttat tgatcatttt gcgggcacca tcgacgacga ccatcgtgcc gtcatccaga
     9781 tacgcgacgc cctgattgta ttccttgcct tcgcgcaaga tgaatacgcg catcgtttcg
     9841 cccggcagca cgacaggctt gagtgcatta gcgagcagat tgacgttcat gatttcgatg
     9901 ccctggagcg tggctacttt gttcaggttg aaatcattgg ttacgatctt cgcatcgtaa
     9961 cgctttgcca gttcgatgag cttgaggtcg acgtccggga tgtgcggaaa gtcgtcttcg
    10021 gcaatttgca catccaagtg ctgcattttc tggatgcgct gcaatacctc gagtccgcga
    10081 cggccgcgct ggcgcttcag cgggtcggcc gaatcggcca cgagttgaag ttcccgcaag
    10141 acgaattgcg gaatcactaa ggcgccgtcg ataaagagcg cgtctgcaat gtccgccaca
    10201 cgcccatcga tgatcacgct ggtatcgagc agcttggtga tgtggcgcga gccgcgctcg
    10261 ctgccgaata cgcctccgaa tgcctggagg ttcaacatat ctcccttgtt cgcaccgagc
    10321 atgaggccta cataggtgga tacgacgaaa atcgacagac tgaagaacga gcgtgtgctt
    10381 tccggcatgg tggtgtggct catgatcagg gaagtgagat atgcgcccag gattcccaga
    10441 atcgagccta cggccgcgcc aatcaatcta cgcagagtga ctttgcggag gcggacttcc
    10501 accaggatga tggcggaggc gaagcaaaca cctgcgacgg tcccttcgac gggcgtgatg
    10561 ctgaatggac ggaagtgaaa accagctacg ccgcagatga cgacaaagat aatgcggatc
    10621 accagggtat cccagaacgt acccactccc ttgcttgcgc gggagagcat tacgggacga
    10681 ggagaaaccg gagtttcggg agacgcgctg ccctgcgtgc ggggaggcgc gttagaaggc
    10741 ctctgcatga tggcaccata accgctataa aggttcagga ggcgctaaca gcaatacgct
    10801 tcccgtattt ctccacggcc gtatgagaga taatccgccc gggccgggta tctatcgacg
    10861 cggcatatat taatgaatac gaggagtata acactcaaaa gcaacgcgga gtcaacgcca
    10921 gcgggcgcgc ggtagtgact cattttgagg ttagccaaaa tacagccttg aaatcgtggt
    10981 tacgggttga atgatttgaa agttcttaga atcattgtct tcaaatcgtg cctgaatcac
    11041 gccagccaac atgtccgtga actggagatt tgagcaattg tggctttcca cgggctgcgt
    11101 cagaaggtta gtcatcgcat ttttacgaaa ccatagttca atttgaagat agtcatggag
    11161 actgtttccg ctttccactt tgatcgagcg cgggtctggg accatggtaa ctacgccatg
    11221 tgttgccatg cgattaagca gagaaagccg aatcatgtaa ttgtagagtt tgttcccatc
    11281 ctgccgtatg tgggccataa cattctgttt gttgaccgta atgctgtgaa ggtgaatatc
    11341 gttatgagcc acacacaact tgtgcgcgga ctcggcaaaa tacgtccttt catcttctga
    11401 taggtcaacc cactttcgtt cgaccctata gggccatttg aagcggttgt agaggtcctt
    11461 gatgagcctt ttcgggagat gcttcttatc cgatggcaca cacagagaag caatagttag
    11521 atatctacta gaccccccgt cgcggtaggg tgcagagaaa ttccaaccca aatcgccgct
    11581 ctcatccacg taaatgacta atgccataga ctccccataa ataaaaaggg cccccaagta
    11641 tggaggcccc aaaagcctaa gggaatggac cctgaatccg acgcgcttac agcgcgctta
    11701 cgatacctga aaggcgtcgc ggtttgctca ggatttaccc attcctgaat ccgtagtgta
    11761 ggcctccgct cgtgagttag caagcatttt ataagggcca tcatttcaat aagttagcta
    11821 cttcttcgat tgtccaagcg tgatccgcaa ttccctcttc catggcggga gtaacgcgta
    11881 gcgtttgatg tatgcggcag aagttgtagt gcatgtagtg aagggcaatt gcccacaggt
    11941 ggttttccag cttctttgag aacgcattcg ttaggcgcgt gaaacggcgc atcttcatgc
    12001 gcatggttag gttctgccgt tcgacatggc tggtagagat gtgctgcgat ttcggctgac
    12061 cgatgattcg cacccgctgc atccgatgca ctctgctgga ctgtagcgcg tttccgattc
    12121 cgcatcgttc ccatacattt taacgagcat ggcgtagtcg atatcgcctc cgaatgcgtt
    12181 ctcgacggca tttagataga cgcggtgtcc gtccgtggtt agctggacgc gattcgcaag
    12241 gcgtgaagcc aaatcgcgca taaaggaata tgcggtctgt gcgccgcgat ctccaaccat
    12301 gaaagacgga acaatcttcg taacagggtc aatcgctacc catgtccaaa catcgcccca
    12361 ggcgtgctcc tctttttgct cgggcgtagc gttcttctct ttcgcgccca cgaggcacca
    12421 aatctcgtca cattgaatca ccttgcaggg aaggttgcga agagccttgt cctgatattc
    12481 gaggcacgcg cggccaatct ccggcacaag ctttagaacg gtgttaaacg cgacatcgca
    12541 catgcgagcc gtggcgcgaa gcgaattacc ttcgatcagg gccgagataa tctgcgcttg
    12601 cttctgtttg ttgagcttgt tcatgataaa cacattctgt gctaaagcgg tttagcatgt
    12661 caagaaaaaa agaaaagggc cacgctgcag acgcagccct ctccgcgctc ttaatgtaag
    12721 acttgaattc ttggtggtgg acgtgctata aacacaatga acctgaggcg caatgcgtct
    12781 ctcctttccg cgctcaagcg aaagattggt tcagcctcgc gccgggggtg cctccccggc
    12841 gtttggcgtt ttaaaccatc gttagcaact gactagctgc tttggacgct ctatcaatac
    12901 ctggattcgt aagccgatat ctcgtgcccc ttctaactcc cgttctcatt attagcccca
    12961 gcccttcggc ccgttgagca aaacggtcca tccggtctat tccgaagccg gactgcttca
    13021 tcccgtttaa cagctcatct gcactgacga gatcagtagc cctcatttct cgctgtccga
    13081 agagtattag taacatgcca tctaattcgc tatcgtcgcc tgtcggccta gccgttagcg
    13141 tcacaagttt gccgtcaacc ttgtatatgc cgttgaattc ggattccgat actaatgtag
    13201 cgtcggctgt gagattggat tgttgtgctt gctggacggg gacaatctgc tgtccggttt
    13261 gagggatgcg ttctgagaaa gagctgtagt gagtctctac aaaatctctc ggaccctccg
    13321 cttccatctc gtaagggccg atctttacac gtaacctgca ttggtccatg tgtgcctcca
    13381 tggtggcatg attatgcaca catgtactga ttcatgtcca gcgttatttt ttgtttttcc
    13441 agcgggcctt ggcggcagcc ttggctcgtt tctttctgcg tgccgctgtc atggtttcca
    13501 tgctgcgttt ccctccgatc ttgccgcctt tgcggcccat ttcggccatg acacgggaga
    13561 gggtttcgcg gtctacttga ggttcttcgg aggtggagag ggctacaact gcggctgcaa
    13621 tctgattagg gtctttaagg ttcttgctaa tacgctttgg catactgata tgatgccacg
    13681 cgagacgggc gggaggcaat atggaccaaa atcaaaatga gtcactaccg ggcgcgctat
    13741 aaccccttcg cttcccggcg gcgcattatg ggtgcagcaa gcgtgcggct acgtgagcac
    13801 gtcacggcga ggatacatac ctgcacttcc gaacgattgt gtatgtgctg ctttctacac
    13861 gtaataacga gggacgcgcc gacctaaggc acagaggaga tcagaggtta cggtgccgat
    13921 ttcgcgagct acatcagaga ctacgatcga atcggcgccg tccttgccat agattgtgac
    13981 gatgtcaccg agttgggctt ccggaacgtc ggtgacgtca agggtcgtga ggtccatgga
    14041 aatggtgccg acaagaggaa cccgcctgcc gcggaccagg acacacccgc gattggtacg
    14101 ctggcgcagg atgccgtccg cataacctgc attgattacg gcaatgcgcg tggggcgttc
    14161 ggtcacaaag cgcgcactat agcccacgcc ttcgcctgcc gggacatcgc gcaaggtaat
    14221 aatgcgggca cgcaatgaga ggcatggcgt gataggcagc ttctcacgga cttcttcgcc
    14281 tttgtgaggc ggatcgaacg attggtagta gccatagagc acggcgccgg ggcgcaccat
    14341 gtccgcaaag gtggtgggac gcgcgcaaat cgctccgcta ttggccatat gaacgatgcc
    14401 gggcgaaacc ccgagctcgc gcatgcgtgc cagacaattg cggaaaagct gctcctgatc
    14461 gtctgtctgg cgggcgctga agtcctcagc ggaagcgaaa tgggtgaacg tcccctcgag
    14521 cgcgatgtgc ttgcattcgg cgagcgctgc cgcgaacgaa tcgatgtctg tagcttcgat
    14581 gccgaggcgg ttcatgccgg tattgatttt caggtgaaag ggaattg
//
LOCUS       DATNDT010000184         4070 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_919579, whole genome shotgun sequence.
ACCESSION   DATNDT010000184 DATNDT010000000
VERSION     DATNDT010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4070
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919579"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1191
                     /locus_tag="VN788_06030"
     CDS             <1..1191
                     /locus_tag="VN788_06030"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXU20123.1"
                     /translation="ATYSQIGAQFGMGLLWTMLFSFPLMAAIQEICARLGRITGTGIA
                     ANLRMHYPKPVLYVVVLLLCVANVFNLGADIIAMGAAAKLVLGGNLNVYAVIFGVLSL
                     CLQIYIPYRRYVRYLKWLTIVLFAYVTTAFVVHVAWIAAIRATVIPSVSWKTDYWMAL
                     VAVLGTTISPYLFFWQASEEAEEVTVTAGESALKKKPFQAFKQFRRIHIDTLVGMAIS
                     NLIAFFIILTASVTIHAAGSAGGIQTAADAAKALQPVAGRLAFLLFSIGIVGTGLLAV
                     PVLAGSAGYAVAETFRWSASLEKKARQAPKFYLIIAGATMIGLSLNFIGLNPIRALYW
                     SAILNGIVATPLMIMMMVISSSRAVVGDFTLPVYLRIVGWAATAVMFLASAGFLMTSL
                     FRKL"
     gene            1440..1844
                     /locus_tag="VN788_06035"
     CDS             1440..1844
                     /locus_tag="VN788_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20124.1"
                     /translation="MTAEAKPQTGREHEVGILTAPLLMLDLNSEIQQLRSEGRWQSGH
                     TAKTLAKYSDFRVVLIVMKTGGRLEKHRTEGRISVQTLEGRIRFTTAERSVDLEPGQL
                     LTLEHDISHDVEGIVDSAFLLTIAWRDASSVR"
     gene            1858..2124
                     /locus_tag="VN788_06040"
     CDS             1858..2124
                     /locus_tag="VN788_06040"
                     /inference="COORDINATES: protein motif:HMM:NF022608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2945 domain-containing protein"
                     /protein_id="HXU20125.1"
                     /translation="MSKTFKHGDHVAWNSEAGRVRGTVVKKVVSDVTFKGYAHHATKE
                     EPQYLIKSDKTDHVAIHQGSALRLLSSRPKTKRGSSARRRKTKR"
     gene            complement(2169..3524)
                     /locus_tag="VN788_06045"
     CDS             complement(2169..3524)
                     /locus_tag="VN788_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HXU20126.1"
                     /translation="MPQVSRIANQRPAWLDDNTKSVMDRAIAREQSLSRLLMIYIVTG
                     LVFMLLPGTFLGVWNLIKISSREAAESISPAWIQAHGHAQLFGWVGTFILGIGFYSIP
                     KLRKLKPFALWSAWLTWALWIIGVTVRWFANIYPWHWRIALPLSAAFELAAFLIFFQA
                     VASHRPSSGSAKPFEPWIFVVITATLGLLASLLLNLGASFQLALNASSPAFPHLFDQR
                     YLIVSTWGFIVPMVWGFSARWLPVFMGLRPLRKSFLLVALGSNTAGVILGFLEHFFAA
                     GILLLAGAATAVAAIRFFEPAVKPAKIINVHRSFPVFIRMAYGWLLIAAAIGIWAARA
                     GEAPGIWGASRHALTVGFIAAMIFCVGQRTLPSFCGMRVLWSPRLMLVMLVLLMTGCT
                     LRVASEILAYQDYAKWAWNILPVSAFIELTAVALFAINLIATFARPPVVAPSPLTMTT
                     E"
     gene            complement(3573..>4070)
                     /locus_tag="VN788_06050"
     CDS             complement(3573..>4070)
                     /locus_tag="VN788_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20127.1"
                     /translation="GSPLRVRKLIPPLTLRQTDGRLVHAWDFKQKKNLVIALLDENCS
                     QCENFIRDLANQASDLREQEAIILLAFLGEPQQSLRDILSDEFFAGAASAPHLRQFLG
                     EDATSAERLSRRGVFVTDRYGEISSMWITQGHDFPRIEQILSALNLVEIACEECEVPF
                     WPVDD"
BASE COUNT          986 a         1101 c         1114 g          869 t
ORIGIN      
        1 gcaacatact cgcaaatcgg ggctcagttt ggcatgggcc tgctttggac catgctgttt
       61 tcatttccgc ttatggccgc catacaagag atctgcgctc gtctggggcg tatcacgggc
      121 accggcatcg ccgctaatct ccgtatgcat tatccgaagc ccgtcctcta tgtggttgtt
      181 cttctgttat gtgtcgccaa cgttttcaat ctaggcgccg atatcattgc catgggtgcc
      241 gctgcgaaac tcgtgctggg gggaaatctg aatgtttatg cagtgatttt tggcgtctta
      301 tctttgtgtc ttcagattta tataccttac cggcgctacg tccgctattt gaaatggctc
      361 acgatcgttt tgttcgccta tgtaaccacc gcgttcgtcg tgcatgtggc atggatagcg
      421 gcaatccgcg ccacggttat cccctcggtc tcctggaaaa cggattactg gatggcgttg
      481 gtcgcagttc tcggcaccac catcagccct tacttatttt tctggcaggc ctcggaggaa
      541 gccgaggaag tgacggttac ggctggcgaa tcggctctaa agaaaaaacc ctttcaggcc
      601 ttcaaacaat tcaggcgcat acacatcgac acgctggtcg gcatggccat ctctaacctc
      661 atcgcatttt ttataatcct gaccgcaagc gtcacgattc acgctgccgg gtccgccggc
      721 ggcattcaga cagccgccga tgccgcaaaa gcgctacaac ctgtcgccgg caggctggcg
      781 tttctgctat tttcgatcgg catcgtcgga acaggattac tggcggttcc ggtattggcc
      841 ggatcagcgg gttacgcggt ggccgaaacg ttccgttgga gcgcgagcct ggaaaaaaaa
      901 gcgcggcaag caccgaagtt ctatctgatc attgcgggag cgacaatgat cggtttaagt
      961 ctgaatttca tcggcctgaa tccgatccgt gctctgtact ggtcggcgat cctcaatggg
     1021 attgtggcca cgcccttgat gataatgatg atggtgattt cgtccagcag ggctgtagtt
     1081 ggcgatttca ctcttccggt ctatctgcgg attgtgggtt gggcggcgac tgctgtgatg
     1141 tttcttgcgt cggcgggatt cctgatgacg tcgctgttcc gcaaactctg atgtccacct
     1201 ctcgacactt ctggcaatgg taccgtctgc cgaattagag acgagggctc tcgaaattct
     1261 gtggttctcg ctaaagtcag catcgaggta ggatacgcca ctggatttga ggcatagccc
     1321 tcccagaaac gttttggcac agtctcgacc agccggcttc cgccccttga atcacacgtg
     1381 ccggtcgccc aaaatgctcg tgcgggcggc accagcatct ccaaaggaga gatgaaatca
     1441 tgacagcaga ggctaagcca caaacaggac gcgaacatga agtggggatt ttaaccgcgc
     1501 ccctcttgat gctggacctt aacagcgaga tccagcaact acgatccgaa ggacgctggc
     1561 agtctggaca taccgcaaag acgctcgcga aatattccga ttttcgtgtg gttctgatcg
     1621 ttatgaaaac tggcgggcgt ttggagaaac atcgaaccga gggtcgaatc tcggttcaaa
     1681 ccctcgaagg gcgaattcgg ttcactacgg ccgagcggtc ggtagatctc gagcctggcc
     1741 aactgctgac gttagaacac gatatttctc acgatgtcga aggcatagtt gacagcgctt
     1801 ttctcctgac cattgcatgg cgggacgcat cttccgttcg atagcaactg aagaacgatc
     1861 tctaagacgt tcaaacatgg ggatcacgtc gcatggaact cggaggcagg tcgagttcgc
     1921 ggtaccgtcg tgaagaaagt tgtttcagat gtgacattca aaggctacgc ccaccacgcc
     1981 accaaagaag agcctcagta tctcatcaag agtgataaga ccgaccatgt cgcaattcac
     2041 cagggctcag cacttcgact tttgagttcg cggcccaaaa cgaaaagggg cagttcggcg
     2101 agaaggcgaa agactaaacg ttgagcggcc tgtcttcgtc gagcgcttcc cgtgcgccca
     2161 gggcttcgct attccgtggt cattgtgagc ggagatggcg caacaaccgg cgggcgcgcg
     2221 aacgtcgcaa tcagattgat agcaaacagt gccaccgccg tgagttcaat gaacgcagaa
     2281 accggcagaa tattccaagc ccatttcgca tagtcctgat aagcgagtat ttccgaagcc
     2341 acgcgcaggg tgcatccggt catcagcagg accaacatta ctagcatcag tctcgggctc
     2401 cagagcacgc gcatcccgca aaacgagggc agggttctct gtcccacgca gaaaatcatt
     2461 gcagcgatga agccaacggt cagagcatga cgcgaggctc cccaaattcc gggtgcctcc
     2521 ccggcgcgag cggcccaaat gccaatagcg gcggcgataa gtaaccagcc atacgccatt
     2581 cggatgaata ccggaaaact ccgatgcacg ttgatgattt tcgccggttt gaccgcaggc
     2641 tcgaagaatc gaatcgcggc aacagccgtc gctgcgccag ccagaagaag tatgccagcg
     2701 gcaaaaaagt gttccaggaa gcccaagatc acaccggcgg tgttcgagcc cagcgccact
     2761 agcaagaaag atttccgcag tggccgcaat cccatgaaca caggtagcca gcgcgcgctg
     2821 aagccccata ccatgggaac gataaaaccc caggtggaga cgatcaaata ccgttgatcg
     2881 aacaggtgcg ggaacgccgg cgaactagca ttgagcgcca gctgaaacga agcgccgagg
     2941 ttgagcagaa gtgatgcgag caggccgaga gtcgctgtaa tcacgacgaa gatccaaggc
     3001 tcaaacggct tcgccgaacc gctcgacggg cgatggctgg ctaccgcttg aaagaaaatg
     3061 aggaaagcgg ccaattcaaa cgcagccgac aagggcagcg caatccgcca gtgccatgga
     3121 taaatgttcg caaaccagcg aaccgtcacc ccgatgatcc aaagcgccca cgttagccat
     3181 gcggaccaaa gcgcaaaggg cttcaacttt cgtagcttcg gtatcgagta aaagccgatg
     3241 ccaaggataa atgtgccaac ccaaccgaac agttgagcgt ggccatgcgc ttgtatccac
     3301 gccggggaaa tggattccgc cgcttcgcgg ctgctgatct tgatgagatt ccaaacgccg
     3361 agaaatgtgc cgggcagaag catgaatact aaccctgtaa cgatatatat catcagcagg
     3421 cgcgagagag attgctcacg tgcgatggcg cgatccatga ccgattttgt gttatcgtcg
     3481 agccaggcag gtcgctgatt agcaattcga gaaacttgag gcataaactt tcgtctctat
     3541 cgagagcaaa atcaccgggg gcttacagaa tctcagtcgt caacgggcca aaaagggact
     3601 tcgcactcct cacaagcaat ctcaacaaga ttaagcgcgc tcaagatttg ttcaatccga
     3661 gggaaatcat gtccttgagt gatccacatg ctggagatct cgccgtaacg gtctgtgacg
     3721 aagacgccac ggcgagacaa tcgttccgcc gaggttgcat cttcaccaag gaattggcgc
     3781 aaatggggag cactggccgc accagcgaaa aactcgtccg acagaatatc gcgcaatgac
     3841 tgctgtggtt cacccaggaa ggccaggaga attatcgctt cctgctcgcg caagtctgat
     3901 gcttggtttg ccaaatctcg aatgaagttc tcgcattgcg agcaattttc atcgagaaga
     3961 gcgatcacaa ggttcttttt ctgcttgaaa tcccacgcat ggacaagcct cccgtccgtt
     4021 tggcgcagag tcagaggcgg gatgagtttc ctgacgcgaa gggggctgcc
//
LOCUS       DATNDT010000185         6390 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1943634, whole genome shotgun sequence.
ACCESSION   DATNDT010000185 DATNDT010000000
VERSION     DATNDT010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6390
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1943634"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VN788_06055"
     CDS             <1..244
                     /locus_tag="VN788_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20128.1"
                     /translation="GLVYLLAIAVEVLSRRRAFQKLGVLRGYKEYLYTTAQKAASSCW
                     CKEKSSTVGVKKTSGGKGIEGDCASRRRWYAVGPAD"
     gene            complement(354..1073)
                     /locus_tag="VN788_06060"
     CDS             complement(354..1073)
                     /locus_tag="VN788_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HXU20129.1"
                     /translation="MKRTLLFLLTLALAGLPTLMATRTARADDKSKDEGRLENAGEVM
                     KEILDIPDDIPQSLLDKADCVIVIPSVLKAAFIVGGSYGRGAMTCRSGDNFRGPWGAP
                     TMMALEGGSFGFQIGAQATDFVLLVMNQGGAKSILSSKVKLGADASAAAGPKGRSAEA
                     DTDVAMRAEVLTYSRARGLFAGISLEGSTLRPDNDSNERIYGKAVDAKDVALHGAVPI
                     PASARALINTLNQHSPKNLSN"
     gene            complement(1223..2518)
                     /locus_tag="VN788_06065"
     CDS             complement(1223..2518)
                     /locus_tag="VN788_06065"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HXU20130.1"
                     /translation="MNTYGWRAALSTALVAAMLMPWTTNAYSVFTHEELIDLTWRSAI
                     RPLLLERFPGSTDAQLRQAHAFAYGGCDAQDMGYYPFGNKFFSQLTHYVRPGDFINNL
                     FRESHNVNEYAFAIGVLSHYIGDSIGHSVAVNPSTGINFPKLAKKFGPIVTYDENPHA
                     HVRTEYAFDIGQLSKRTFAPPAFMRFIGFRVPRGLLERAFVATYGIEVRELVGKTLPA
                     MKSYRSSVRTLIPIFSNAEIVLHGKQFAPDTDNAEYRHFVNQLRQADYERHWAYTYRR
                     PGLGSHLLAIIIKIIPKIGPASYLAIKIPDAQTESWYIESVNRSADDYRRLLDRLKIH
                     PTAVLDLPNRDLDTGAKVKAGEYALTDDTYAKLVDRLTSDPNRTIPNGLRENILEYYG
                     DLSAPISTKKHPREWQKLTSELQVMTEMKSAEVMATPTE"
     gene            3217..5064
                     /locus_tag="VN788_06070"
     CDS             3217..5064
                     /locus_tag="VN788_06070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20131.1"
                     /translation="MRALAACAAVLGLIVSPAITRAGRPGDKDSAANATPASAADAAA
                     KAAPSSMEVESDLRQMRDLLEAQARQLQEQQQRMNQLEEQLKASQPGLISESPAAAPA
                     NSASEPVAPVGPVGPATGVVAASQASQEKNPDEPTSLHFKGITLTPGGYMAAESVWRQ
                     HSLSADVNTPFNSIPFAGSPMAHTTEFNASGRQSRIAMLAEGKLSGVKIGGYYEADFL
                     SAGVTSNYNESNSFTLRQRQFWGQAAFASGWTVTGGQMWSLMTETKKGMDNRTEAVPL
                     TIDAQYHVGFGWARQYGFRLTKNFSNKFWLGASVENAETTTAFHGQNNDFLVGAPGSG
                     GGLLNPIANFAFTKAPDFIVKAVVEPGWGHYEIFGVVSTFRDRIYPCGGTAAGVACGG
                     LIGPSGAGAFNDTRVGGGVGANARVTVLKKIDLGIHFMGGDGIGRYGAGSLPDVTVRP
                     DGTLALIRSFQSLGTIELRATPKLDVYMYVGGEYAARTQFLKGGTFSEGYGAIALPNF
                     GCSVEQAPGSAVSTPTGVGGSGGFVPGSLASCTGDTRNVIEGTFGFWYRFYKGSKGTV
                     QWGPQYSYLRRNTWNGVGTGSVNGLTVTTNGAPHGVENMVFTSFRYVLP"
     gene            5218..6102
                     /locus_tag="VN788_06075"
     CDS             5218..6102
                     /locus_tag="VN788_06075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20132.1"
                     /translation="MKRKTFRTIVMLALSVLLIGVLQAKAGPPFQTDDPEPVDFHHYE
                     FYTFGNTDGTGVETDSSGPAVEFNWGILPNTQFHIVVPAVAIAPSNNPKYFPSGAGQS
                     AFGMGDIEIGVKYRYVKETKYRPMIGTFTIIEVPSGNSNRGLGVGKVWAKLPVWLQKS
                     FGPWTTYGGVGEQIVPQTGYKNFTYGGWLLERDIGKKWTLGTEVFSHGREGIATPLIR
                     ASTLVDFGGYYYIRNPGFQVLFCYGHAVAGMPETYGYLGLYWTWGGAKDDPQPAKSLL
                     EKLHRLQIPVLNAKFSED"
BASE COUNT         1380 a         1713 c         1857 g         1440 t
ORIGIN      
        1 tgggctggtg tacctgcttg cgattgcagt ggaagttctg tctcggaggc gggccttcca
       61 gaaactgggc gtacttcgag gatacaagga atatctttac accacggccc aaaaggctgc
      121 aagtagttgt tggtgtaaag aaaaatcgtc cacggttggt gtaaagaaaa cgtccggggg
      181 gaagggcatc gagggcgatt gtgcatcccg aaggagatgg tatgcggtcg gtcccgcgga
      241 ctgacgcggc ctcgatttag ccggcgcgcg cgaggaatac tttgagcgct tcaacggtaa
      301 gcgacgcgta tagtgcacag cgcttggcga tcagcgtgtt atggaatcgt gagttaattc
      361 gacaggttct tgggactgtg ttggttcagg gtattgatga gagctctcgc cgaggccggt
      421 atcggaacgg cgccatgcag cgcgacgtcc ttggcgtcca ccgcctttcc gtagatccgc
      481 tcgttggagt cgttatccgg acgcaaagtc gatccctcaa gagaaatacc ggcgaagagg
      541 ccgcgcgcac gggagtacgt gagaacttcg gcacgcatgg ccacgtcagt atctgcttcg
      601 gcagagcggc ctttcggtcc agccgcagct gacgcgtctg ctccgagctt cactttgctg
      661 ctcagaattg acttcgcacc tccctgattc atcaccagga ggacgaaatc tgtcgcctgg
      721 gcgccgatct ggaaaccgaa gctgccgcct tcgagggcca tcattgttgg cgcgccccat
      781 gggcctcgga aattatcgcc gctgcgacag gtcatcgctc cgcggccata acttccacct
      841 acgataaaag ccgccttgag aactgacgga atgacgatca cgcaatcggc cttgtccaac
      901 aggctctgcg ggatatcgtc tgggatatcg aggatttcct tcatcacttc cccggcgttc
      961 tcgagccggc cctcatcctt tgatttatcg tccgctcgcg ccgtgcgggt ggccatgagc
     1021 gttggaagac ccgcgagcgc cagcgtcagc agaaaaagca gtgttctctt catgttcacg
     1081 agccgcctcc tgaattcaga cctacatcat aagtgggccc gcacggcaac gcaacctttg
     1141 cgggatcagg gcgcccaaac ggggaaattt caggcaattc cgcgtggaag ttcttggtgc
     1201 catgtctgca tcttcaaatc agttattcgg tcggcgtcgc catgacctcg gctgatttca
     1261 tttccgtcat cacttggagc tcggacgtaa gcttttgcca ttcgcgagga tgtttctttg
     1321 tgctaatcgg cgcgctgaga tccccatagt actccaagat attttctcgc agcccgttgg
     1381 ggattgtccg atttgggtcg gaggtcaagc gatccaccag cttcgcatag gtgtcgtcag
     1441 tcagagcata ttcaccggcc ttcactttcg cgccggtgtc gaggtctcga ttcggaagat
     1501 caagtaccgc ggtcggatga atcttcagcc gatccaggag gcgccggtaa tcgtcggcag
     1561 aacggttgac actttcgatg taccaagact ccgtttgcgc atcgggaatc ttgatcgcca
     1621 agtacgaagc cgggcctatc ttggggatga ttttgatgat gattgcaagc agatgcgatc
     1681 ctaagccggg ccttcggtat gtataggccc aatgacgctc gtaatctgcc tggcggagct
     1741 gattgacaaa gtgacggtat tcagcgttgt ccgtatccgg cgcgaattgt tttccatgca
     1801 gaacaatttc ggcgttggaa aaaatcggta tcagagtgcg aacggagctt cggtagctct
     1861 tcatagccgg caaagttttg cccaccagct cgcgaacttc gattccgtaa gtggctacga
     1921 aggcgcgttc caacaggccg cgcgggactc ggaagccgat gaaccgcatg aatgccggcg
     1981 gagcaaaagt gcgttttgaa agctgtccga tatcgaatgc gtactccgtg cgcacatgtg
     2041 catgtggatt ttcgtcatag gttacgatcg ggccgaattt tttggcaagt ttgggaaagt
     2101 tgattcccgt ggagggattg acggcgaccg agtggccaat cgaatcgccg atataatgcg
     2161 acaagacgcc aatcgcaaag gcatactcat tcacattgtg cgattctcta aataaattgt
     2221 tgatgaaatc gccggggcgt acatagtgtg tcagctgact gaaaaacttg ttgccgaacg
     2281 ggtagtaacc catgtcttgc gcatcgcagc caccgtaggc gaaggcgtgc gcctggcgta
     2341 attgcgcatc ggtggaaccg ggaaaacgtt cgaggaggag cgggcggatc gcgcttcgcc
     2401 acgtgagatc gattagctct tcgtgtgtga acaccgagta cgcattggtc gtccacggca
     2461 tcaacatagc ggccaccaac gcagtcgata gagcagctcg ccagccgtaa gtattcaact
     2521 gctcgcctcc gtacaagctg tggagcttca gatgcgctgc gcttatttcc agcgccagcg
     2581 tctgtctccc gaatgcattc tcgcaccctc agacaaaaga ttaacagtgg cactcgtcat
     2641 ttcccatttc atgcttgcgt ctccgatacg ggcggcgtgg atggtggttc gacccttggg
     2701 agcacgtacg gctttattgc cggcaccatc cccaacagcg agcgcgagat ccaatttgcg
     2761 ttgaaattga tattctgatc gtggcagcga tccaacggaa gtggaccact gccacattca
     2821 gaattgcaga aggtattgcg tagcaggttc ctgctacccc gccaataacg ggcattcaaa
     2881 tacgggaagt cacgcaatta ataatttccg tcaatgtgcg ctcaagcggg acgcgtagcg
     2941 tccgtctagg aatttcgtga gccggaattc tgggcggacc taatttcgag cgcccgttgc
     3001 aagtgcgtgc tccttgtgct agtttctgtg gcgcaatgaa accgcgcgtg catcactcct
     3061 ccggcttcac ttgaatctga cctcagaaga cgccagtttg tcgttttcgc gttcgtaggc
     3121 attccaaatt cacagtcagt tggctgcgct taatatcggc ttaagtattc acgtcggcgc
     3181 cgtcgaccat ccgtaattaa tttcaaggag gaaataatgc gagcgcttgc agcatgtgca
     3241 gcggtcctcg gcctaatcgt gtcgccagca atcactagag caggccgtcc aggagacaag
     3301 gactcggcgg ccaatgctac gccggcatcg gcggctgacg cagccgcgaa ggcggctccg
     3361 tccagcatgg aagtggagag cgatctgcgg cagatgcgcg atcttcttga agcgcaagcc
     3421 aggcagcttc aggaacagca gcaaaggatg aatcaactcg aagagcagct caaggcgtcg
     3481 cagccagggc taatcagcga gagcccggcg gccgctcctg caaactccgc gagcgaaccg
     3541 gtcgcgccag ttggaccagt tggaccggcg acgggcgtcg tggcggcttc acaagcctct
     3601 caggaaaaaa atccagacga accgacttct cttcacttta aagggatcac tcttacgccg
     3661 ggtggttaca tggcggccga atcggtttgg cgccagcact ctctcagtgc agatgtcaac
     3721 acgcccttca actcgattcc gttcgccggc agtcctatgg cgcacacgac ggagttcaac
     3781 gcttccgggc gccaatcgcg gatagcgatg ctcgctgagg gcaagcttag cggtgtgaaa
     3841 atcggcggct actacgaagc ggacttcctc tctgcgggcg tcacctcaaa ttacaacgag
     3901 agcaacagct tcaccttgcg tcagcggcag ttctggggcc aagccgcttt cgccagcggc
     3961 tggaccgtca cgggcgggca gatgtggagc ttgatgacgg aaaccaagaa gggcatggat
     4021 aaccggaccg aggccgtgcc tctgacaatt gacgcgcaat atcacgtcgg cttcggctgg
     4081 gcgcgccaat acggtttccg cctcactaaa aactttagca ataaattctg gctgggcgct
     4141 tctgtagaaa acgccgagac taccacggcg ttccatggcc agaacaacga ctttctcgtt
     4201 ggagcacccg gatccggcgg cggtctcctg aatccgattg ccaattttgc cttcaccaag
     4261 gcgccggatt tcattgttaa agcggttgtt gaaccgggct ggggtcatta tgagattttc
     4321 ggtgtcgtga gcacattccg ggatcgcatt tatccctgcg gcggaacagc cgcgggagtt
     4381 gcttgcgggg ggttgattgg tccttccgga gcgggcgctt ttaacgacac gcgcgttggt
     4441 ggtggtgtgg gtgccaatgc tcgcgtcacc gtcctcaaga aaattgatct cggcattcac
     4501 ttcatgggag gtgatggcat aggtcggtac ggggcaggtt cactccctga cgtgacggtc
     4561 cgtcctgatg gcacgcttgc gctgatccgc agcttccagt cgctcggcac gatcgaactg
     4621 cgcgccacgc cgaagctcga tgtgtacatg tacgtcggcg gggagtatgc agcccgcacg
     4681 caattcctga aaggcggaac gttcagcgaa ggatacggag cgattgcact gcccaatttt
     4741 ggctgttccg ttgagcaagc cccaggctct gccgttagca ctccaactgg cgtcggcggg
     4801 tcgggaggtt tcgtccccgg ttcgctggct agctgcaccg gagacacgcg aaacgtgatt
     4861 gaaggtactt ttggtttctg gtatcggttc tacaaaggct cgaagggcac tgtacaatgg
     4921 gggccacaat actcctacct ccgacgcaat acctggaatg gggtgggaac tggctcggtg
     4981 aacggactga ctgtgaccac caatggcgca ccccacggcg tcgagaacat ggttttcaca
     5041 tccttccgtt acgtcctgcc gtgagggcgc acgtcagtga gcgaagacgg ggacagagct
     5101 ttttcgccct gtgtctccgc gccctgctac cctctgtata gttgggtgtt cgaattaggg
     5161 gcgtgtaaaa gcggtttctt ctgaatccct gttgaaaaac cttggagaga ggctaggatg
     5221 aagcgaaaaa catttaggac aatagtgatg ctggcgctat cggtccttct tatcggtgta
     5281 ttgcaggcga aggcaggtcc tccctttcaa accgacgacc cagaaccagt ggacttccat
     5341 cactatgaat tttatacgtt tggcaatacc gatgggaccg gcgtagagac ggactcatcg
     5401 gggccggcag tcgaattcaa ctgggggatt cttccaaata cccagtttca tattgtagtt
     5461 ccagcggtgg ccatcgcgcc ctcgaacaac ccaaagtact ttccgtctgg tgctggacaa
     5521 agtgcctttg gcatgggcga cattgaaatc ggtgtgaaat accggtacgt aaaggaaacg
     5581 aagtaccgcc cgatgattgg cacgttcacc atcatcgaag taccaagcgg aaactcgaat
     5641 cggggactgg gtgtcgggaa agtgtgggcg aaactgcccg tctggttgca aaagagcttt
     5701 ggtccctgga cgacctacgg cggcgtgggt gagcagatcg ttccgcagac aggctacaag
     5761 aattttacgt acggcgggtg gttgttggag cgggatatcg gcaaaaagtg gacattaggc
     5821 accgaggtct tctcccatgg ccgagaaggg atagcaacac cgctaattag agcgtcgacg
     5881 cttgtggact tcggcggata ttactacatc cgaaatcccg gctttcaagt ccttttttgc
     5941 tacgggcacg cagtggcagg catgccggaa acgtatggtt atctcgggct gtattggacg
     6001 tggggaggtg ctaaagatga cccgcagcca gccaagagtc ttctcgaaaa gcttcaccga
     6061 ttgcaaatcc cggtactaaa cgcgaagttt tccgaagatt aagtcagttt cttcaagaat
     6121 aattgaatcg gctgcggcat cacacactgc ctgccgctac tctccacgtg tgcggatacc
     6181 cgatgatgcc gtctgctaca atcagcggct cagcctgggt ttcaccgcga tatgaatcaa
     6241 atccgcctac cacgctggtt ggtgaagatc gttgccgtga tgggcggcgg cggattgttc
     6301 gtcgctactt ttctcgattc gtcggtaatc actcttccat ttatcgccga cgctctggtg
     6361 atccggctgt cggcgcaacg gccggcgcgg
//
LOCUS       DATNDT010000186         6834 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_673234, whole genome shotgun sequence.
ACCESSION   DATNDT010000186 DATNDT010000000
VERSION     DATNDT010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6834
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673234"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..871
                     /locus_tag="VN788_06080"
     CDS             74..871
                     /locus_tag="VN788_06080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HXU20133.1"
                     /translation="MPLAIEQIRRMRGGAQSHLMRCSDEAYYVVKFQNNPQGVRILAN
                     ELLGTRLAARIGLPTPVAAVVEVRENLIEQTEDLVIQLGRGRLRCRAGLQFGSRYPGA
                     PAETVVYDFLPDEQLREVVNLSDFCGMLVFDKWTCNTNGRQAIFLRDKGSSRYRAEMI
                     DQGFCFNAGEWKFPDAPLRGLYARHRVYESVRGIEAFDPWLARVETKMSEEIVEEALS
                     EIPPEWCGSERDALERMAEQLLRRRTLVRQLIISAWKSSAQPFPNWR"
     gene            874..1698
                     /locus_tag="VN788_06085"
     CDS             874..1698
                     /locus_tag="VN788_06085"
                     /inference="COORDINATES: protein motif:HMM:NF022681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3037 domain-containing protein"
                     /protein_id="HXU20134.1"
                     /translation="MPDETRSMLRYRILRYTPNLVRDEWVNIGVLLEAGAGEIEGNGG
                     ALRRLAIQVIEEESEMARVRRLHPGADEALLRALPAEFDARLRAPDAGGYLDKLDSTL
                     SNALQFSPPRGLLAENFDAELARLFREHVAAPPGARGIGQNVRNWMRARMADVFRRRR
                     ILGKLDYRVPVEEFTHPGDPLKLDYGYRYNGTRGYLHAIALDRDVSQSKVLAYTAESI
                     RARMPNSIFTAVTNADPSPENSRHRFIVETLRGQDIAILPLGQIDQFAEELRPRLQ"
     gene            1938..3947
                     /locus_tag="VN788_06090"
     CDS             1938..3947
                     /locus_tag="VN788_06090"
                     /inference="COORDINATES: protein motif:HMM:NF004952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXU20135.1"
                     /translation="MSPEIDHKPEPSALEPPAGEPAANNFPAAPAPAAQAQSASGAAT
                     AVARAQEQPASVAPASDYDVSQASDANQESVPPHENAAAEIEGSETALPAETSAPAET
                     VEATETMDQLLDQFSAPQPAGEGEIFDGRVLAVTDAGVVVDVGGKFEGLVPAQEFLDS
                     GNPIEFGSGQTIEVERLHERKDGYVLLSYARAHRRRVWERIEKSYREHTNLTGKTVER
                     IKGGLVVDIGVRAFLPASQIELRPVHDLEGWKDRDIEIRVLKLNRKRGNVVVSRRAIL
                     EEEQKAKRDSLAETLIEGTVVTGHVKNVTDYGVFIDLGGLDGLLHVSDLAWGRAPHPS
                     SVVKAGDEIQVQILKFDKEKGRISLGRKQLLPDPWATVPERYPAGTRVHGKTVAVTDY
                     GAFVQLETAVEGLVHVSEMSWSKHTKHPSKILKVGDEVEVVVLDVKPDLRRISLGLKQ
                     TLPDPWQEAAQKYPVGAIVSGRIRNLAEFGAFVELEEGMEGLIHISDVSWTERIKHPS
                     EKFKKGEVVEAKVLKLDPQNRRLSLGVKQVHDIWAEWFAAHKIGEVLKGKVARTADFG
                     AFVELAEGIEGLCHISEIEERRPKGERDKAKASRDGRGSSLLAPGQEYDFKILRLEPE
                     QRKISLSYRAAQKQAERHEIQTFRSSKSSPNATIGDAIMAKRQSS"
     gene            4137..4457
                     /locus_tag="VN788_06095"
     CDS             4137..4457
                     /locus_tag="VN788_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXU20136.1"
                     /translation="MTKADLVEEVTRITELPRKESEAVVETIFESIITALQADDKIEI
                     RGFGSFRTRERRGRTGRNPKTGAKVEVPAKKIPFFKPSKELKDYVNTQSAAGAASESH
                     GARD"
     gene            4464..4946
                     /locus_tag="VN788_06100"
     CDS             4464..4946
                     /locus_tag="VN788_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HXU20137.1"
                     /translation="MDYLWTPWRYRYIADAAKDKGCIFCEAAAANDDARTLVVLRGTK
                     NFVILNRYPYTSGHVMVVPYAHVAELAAAEADTLAEMMRIAQRVQVALGKTYHPEGYN
                     VGMNLGRAAGAGITGHIHLHVLPRWAGDTNFMTVAGETRVEPEELSTTFERLRKALAA
                     "
     gene            4915..5748
                     /gene="pcaH"
                     /locus_tag="VN788_06105"
     CDS             4915..5748
                     /gene="pcaH"
                     /locus_tag="VN788_06105"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007026593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HXU20138.1"
                     /translation="MSGCAKPSRLDASARGMHNGARSREFPIARSSSNSSAKSSESLI
                     VKPYRRYPRNSQPLYLYPPYRLTISRAPRKPLIVLPQTLSEVTGPVFGDDAIGETDND
                     LTRQHEGEPLGERITVSGRVLDDNGRPAPNTLIELWQCNAAGRYRHRIDQHQAPLDPN
                     FSGAGRTLTDAQGNYRFITIKPGPYPWENHPNAWRPAHIHFSLYGTAFATRLVTQMYF
                     PGDPLFPFDPIFNSVRDERARQRMIARFEINRTEPGWALAFRYDIVVRGRESTPFERK
                     K"
     gene            5732..6328
                     /gene="pcaG"
                     /locus_tag="VN788_06110"
     CDS             5732..6328
                     /gene="pcaG"
                     /locus_tag="VN788_06110"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HXU20139.1"
                     /translation="MSARSKAGRRLEPTPWHTVGPYFGITVNGANSIRSIAPPDAKGE
                     RVRLICAVFDRDGKCVPDTLIEIWQANGNGKYNHPADTQAKPIDPGFAGFGRQITDEN
                     GECEFETIKPGRVPGPHGALQAPHLVVGVFSRGVMKHLVTRIYFSGDAGNDGCPVLGL
                     VPKARRTTLMARQVAGQPGTWQHEIYLSGPKETVFFDI"
     gene            complement(6460..>6834)
                     /locus_tag="VN788_06115"
     CDS             complement(6460..>6834)
                     /locus_tag="VN788_06115"
                     /inference="COORDINATES: protein motif:HMM:NF014794.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXU20140.1"
                     /translation="RVVCAVGDGSAQYSIQALWSAVQQKAPVIFLVICNGNYSALKGF
                     RDFTQVGKNVPGIDIPGIDAVKIAQGYGMNARAVDRAEDLEPALKCAFASHEPILISV
                     NVQQGGQKTMGMDQSVNPPNYG"
BASE COUNT         1452 a         1864 c         2249 g         1269 t
ORIGIN      
        1 ttccgtccta ttttcctctt tgggaaatgt tccgcttgcc gcgagtgtat gcgtgtagct
       61 agcataccgc gcgatgccgc tggcgattga gcagattcga agaatgcggg gaggcgcgca
      121 atcgcatctg atgcgctgtt cggacgaagc gtattacgtg gtgaaattcc agaacaaccc
      181 ccagggagtg aggatcctgg cgaacgaatt gctaggaact aggctggcgg cgcggattgg
      241 gctgccgacg ccggtggcgg cggtggtgga agtccgcgag aacctgatcg agcagaccga
      301 ggatcttgta attcaactgg ggcgcggaag gctgcggtgc cgcgcggggc tgcagttcgg
      361 ttcccgatat cccggcgcac cggcggagac ggtggtgtac gatttcctgc cggatgagca
      421 actgcgcgag gtggtgaatc tttccgattt ttgcgggatg ctggtgttcg acaagtggac
      481 ctgcaacacc aacggcaggc aggcgatttt tctacgcgac aagggttcat cgcggtaccg
      541 cgctgaaatg atcgaccagg gattctgttt caacgcggga gaatggaaat ttccggacgc
      601 gccgctgcgg ggattatacg cgcggcatcg cgtgtatgaa tcggtacggg gaatcgaagc
      661 gttcgatccg tggctcgcgc gcgtggaaac taaaatgtcc gaggaaattg tggaagaagc
      721 gcttagcgag attccacccg agtggtgcgg ttcggagcgc gacgctctgg aaagaatggc
      781 cgagcaattg ttgcgcaggc gcacgctggt gcgtcaatta attatttccg cctggaaatc
      841 gtctgcgcag ccttttccca attggaggta aacatgccgg acgaaactcg aagcatgctt
      901 cgctatcgga tcctgcgata cacgccgaat ctggttcgcg atgaatgggt gaatatcggc
      961 gtgctgctgg aggcaggcgc gggcgagata gaaggcaacg gcggcgcgtt gcggaggctg
     1021 gccatccagg tgatcgagga agaatctgaa atggcgcgcg tgcgacgatt gcatccgggc
     1081 gccgacgagg ctctgctgcg ggcgctgccc gcggagttcg acgcgcgttt gcgtgcgccc
     1141 gacgcaggcg gctacctgga caaactcgac agcacacttt caaacgcgct gcaattcagc
     1201 cctccgcgag gcttgctggc ggaaaatttc gacgcggagc tggcgcggct ttttagggaa
     1261 cacgtggccg ctccgcccgg ggcgcgcgga atcgggcaga acgtgcggaa ctggatgcgc
     1321 gctcgcatgg cggacgtatt caggcggcgg cgaatcctgg ggaaactgga ctaccgcgtt
     1381 ccagtggaag aattcacgca tccaggggac cctctgaagc tggattacgg ctaccgctac
     1441 aatggcacgc ggggctacct gcacgcgatc gcgctcgacc gggacgtatc acaatccaag
     1501 gtgcttgcat acaccgcgga atcgatccgc gcgcgcatgc cgaattcgat tttcacagct
     1561 gtgaccaacg ccgatccgtc gccggaaaat tcccggcatc gatttattgt tgagacgctt
     1621 cgagggcagg acattgccat tctgccgctg gggcagatcg atcaattcgc tgaagaatta
     1681 cgtccccgtc tacaatgacc agccgcgccg agaccgccga aatcgctgga acgcgaagga
     1741 ccacaaaaga gtgccatgct ggaatttttt gcgcttgtcc gttttgttga caatgcgcaa
     1801 acgcggcgcg tatgatttct gcgatgcagc gccctgcgtg gtgttgtatt cccttctctg
     1861 ctctagcccc gcgggtggag tgaccggggg ccgaagactc agtaataggg cacgaatccg
     1921 tacggaggcc gctttccatg tcacccgaaa tcgaccacaa gccggagcca tcggctctcg
     1981 aacccccggc cggagaaccc gcggcgaata attttcccgc agcgcccgcg cccgctgcgc
     2041 aggcgcagag cgcgtccggc gcagccacgg cagtcgcgcg agcccaagag cagccggctt
     2101 cggtcgcgcc cgcttccgat tacgatgtga gccaagcatc cgacgccaac caagagtccg
     2161 ttccgccgca tgaaaacgcc gccgccgaaa tcgaggggag cgaaacagcg cttccggctg
     2221 aaacgagcgc gccggccgag acagtcgaag ctacggaaac aatggaccaa ttgctggatc
     2281 aattttcggc tccgcagccg gccggcgaag gcgaaatttt cgatggacgc gtgctggccg
     2341 tgacggatgc gggcgtggtg gtcgatgtgg gcgggaagtt tgagggtttg gttcccgcgc
     2401 aggaatttct cgattcgggc aacccgattg aattcggctc ggggcagacg attgaagtgg
     2461 agcggctgca cgaacgcaag gacggctacg tgctgctctc gtacgcgcgc gcccaccggc
     2521 ggcgggtgtg ggagcgcatt gagaagtcct accgcgagca caccaacctg accggaaaaa
     2581 cggtggagcg catcaagggc ggcctggtgg tcgatatagg cgtgcgcgcc tttctgcccg
     2641 cttcgcagat cgagcttcgt ccggtacacg acctggaggg ttggaaggat cgcgatatcg
     2701 agattcgcgt gctgaagctg aaccgcaagc gcgggaatgt ggtggtgagc cggcgcgcca
     2761 ttcttgaaga agagcagaag gccaagcgcg attcgctggc cgagacgctg attgaaggca
     2821 cggtggtgac cggacatgtg aagaatgtga ccgattacgg cgtgttcatc gacctgggcg
     2881 gtttggacgg gctgctgcac gtgagcgatc tggcatgggg acgcgcaccg catccatctt
     2941 cggtggtgaa ggcgggcgac gaaattcagg tgcaaattct gaagttcgat aaggagaagg
     3001 ggcggatttc actgggccgc aagcagcttc tgcccgaccc gtgggcgaca gtgccggaac
     3061 gatatcctgc gggaacgcgg gttcacggaa aaacagtggc ggtgacggat tacggcgcgt
     3121 tcgtgcaact ggagacggcg gtggagggat tggtacacgt cagcgaaatg agctggtcga
     3181 agcacaccaa gcacccttca aagattttga aggtgggcga cgaagtagag gtggtggtcc
     3241 tggacgtaaa gcccgatttg cggcgcattt cgctggggct gaagcagacg ctgcccgatc
     3301 cgtggcaaga agcggctcag aaatatccag tgggggcgat tgtgagcggg cgcatccgca
     3361 acctggcgga gtttggcgcg ttcgtggaac ttgaggaagg gatggaaggg ctgatccaca
     3421 tcagcgatgt gagctggacg gagcgcatca agcatcccag cgaaaagttc aagaagggcg
     3481 aagtggtgga agcgaaggtg ctgaagctcg acccgcaaaa tcggcggctg tcgctgggcg
     3541 tgaaacaggt gcacgacatt tgggcggagt ggtttgccgc gcacaagatc ggcgaagttt
     3601 tgaaggggaa agtggcccgg acggcggatt ttggggcgtt cgtggaactg gcggaaggca
     3661 tcgagggact ctgccacatt tcagaaatcg aagagcgtcg gccaaaagga gagcgcgata
     3721 aagcgaaagc gtcgcgcgac gggcgcggca gttcattgct cgcgcctggg caggaatacg
     3781 actttaagat ccttcggctg gaaccggaac agcgcaagat cagcttgagc tatcgcgccg
     3841 cacagaaaca ggctgagcgg cacgagatcc aaacattccg ctcgtcgaaa tcatcgccca
     3901 acgccacgat tggcgacgcc atcatggcga aacggcagtc gtcatagcga atcccaggat
     3961 cgcttgctaa gataagtcgg tggctagaaa gtcatttacc tagccgtgaa tcgatggtat
     4021 ccaaagattg tgtggtgctt tggcccagcg caagcggcgc gtgcggtgtg aaatcgtgag
     4081 aggaggcccc aaagatggct gaggctgccg aacagcgcgg aaacacggaa cacacgatga
     4141 ccaaggcgga tttggtggag gaagttacgc ggatcaccga actcccgcgc aaggaatccg
     4201 aggcagtggt cgagacgatt ttcgaaagca tcatcacggc gttgcaggcc gacgacaaaa
     4261 tcgaaattcg cggcttcggc agtttccgca ctcgcgaacg ccgcgggcgc accggccgca
     4321 atcccaaaac aggggccaaa gtggaagtac ccgcgaagaa aatccctttt ttcaaaccca
     4381 gcaaggaact caaggactac gtcaacacgc agagcgcggc gggagccgct tccgagtccc
     4441 acggcgcgcg ggactgaccg gaaatggatt atttgtggac gccctggcga tatcgctata
     4501 tcgccgatgc cgcgaaggac aagggctgca ttttttgcga ggccgcggcg gccaatgacg
     4561 atgcccgcac gctggtggtt ctacgtggca ccaagaattt cgtaatcctg aatcgctacc
     4621 cttacaccag cggccacgtg atggtggtcc cttacgcgca tgtggccgaa cttgcggcgg
     4681 cggaggcgga cacgcttgcg gaaatgatgc ggatcgcgca gcgcgtccaa gtagcgcttg
     4741 gaaagaccta ccatccggag ggatataacg tcgggatgaa tctcgggcgc gcggccggag
     4801 caggaataac agggcacatt catttgcacg tgcttccgcg gtgggcggga gacacaaatt
     4861 tcatgaccgt ggcgggggaa actcgcgtgg agccggagga gctttcgacc acgtttgagc
     4921 ggctgcgcaa agccctcgcg gcttgatgcc tctgcgcgcg ggatgcataa tggggcgcgt
     4981 tcacgggagt ttcccatcgc acgcagttca tcgaattcgt ccgccaaatc ttcagaatcg
     5041 ctgatcgtca agccctatcg gcggtatccg cgcaattcgc agccgctgta cttgtacccg
     5101 ccgtatcgtt tgacgatttc tcgcgcgccg cgaaagccgc taatcgtcct gccgcaaacg
     5161 ctttcggagg tgaccggtcc cgtgttcggc gacgacgcga tcggagaaac ggataacgat
     5221 ctgacgcgcc agcacgaagg cgagccgctg ggtgagcgaa tcacggtgag cgggcgggtg
     5281 ctcgacgaca acggccggcc ggcgcccaac acgctgatcg aattgtggca atgcaatgcc
     5341 gccggacggt atcggcacag aatcgaccag catcaggcgc cgctcgatcc aaatttttcc
     5401 ggagcggggc gcactttgac ggacgcgcag ggcaattatc gcttcatcac gatcaagcca
     5461 ggaccgtatc cctgggaaaa tcatccgaat gcctggcggc cggcgcacat tcatttttcg
     5521 ctttacggca ctgcttttgc gacgcgactg gtcacacaga tgtattttcc gggcgatccg
     5581 ctttttccgt tcgatccgat tttcaattct gttcgggacg agcgcgcgcg ccagcggatg
     5641 atcgcccgct tcgaaatcaa ccgcaccgag cccggttggg cgctcgcatt ccggtacgac
     5701 attgtggtgc gcggaaggga gagcactcct tttgagcgca agaagtaaag ccggccggcg
     5761 gctggaaccg actccgtggc acacggtggg accgtatttc ggcatcacgg tgaacggcgc
     5821 gaattccata cggtccatcg cgccgccgga cgcgaagggc gagcgcgtga ggctcatctg
     5881 cgcggtgttt gatcgcgacg gcaagtgcgt gccggacacg ctgattgaaa tctggcaggc
     5941 gaacgggaac ggcaagtaca accatccggc cgacacgcag gcgaagccga tcgatcccgg
     6001 attcgcggga ttcggccgcc agatcacgga cgagaacggc gagtgcgaat tcgagacgat
     6061 caagccgggc cgcgtgccag ggccgcacgg cgctctgcaa gcgccgcacc tggtggtagg
     6121 agtcttcagc cgcggcgtga tgaaacatct ggtgacgcga atttactttt ctggcgacgc
     6181 cgggaacgat ggatgtcctg tacttggttt ggtgccgaag gcgcggcgaa cgactctgat
     6241 ggcgcggcaa gttgcgggac agcctggaac ttggcagcac gagatatatc tgagcgggcc
     6301 gaaggaaacg gttttcttcg acatctagaa cgctttcgcg ggcgctcctc actttgaagt
     6361 gcatcacttt ggaacgggat tggaggcgaa ggcccacgat gaggaaggtc cgtcgtggct
     6421 accgctttcg gccagcagcg gcgctacgaa agccaaaggc tatccgtagt tcggcggatt
     6481 gacggactgg tccatgccca tggttttttg gccgccttgt tggacgttga cgctgatgag
     6541 gattggctcg tgcgaggcga aggcgcattt cagcgccggt tcgaggtctt cggcgcgatc
     6601 gacggcgcgc gcgttcatgc catagccttg cgcgattttt acggcgtcga ttccgggaat
     6661 gtcgatgcca ggtacatttt ttccgacctg cgtgaaatcg cgaaaacctt tcagcgcgga
     6721 ataatttccg ttgcagatca cgagaaaaat cacgggcgct ttctgctgca cggcggacca
     6781 tagcgcctga atcgaatatt gcgccgagcc gtcaccgacc gcgcagacca cgcg
//
LOCUS       DATNDT010000187         4761 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1253153, whole genome shotgun sequence.
ACCESSION   DATNDT010000187 DATNDT010000000
VERSION     DATNDT010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1253153"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VN788_06120"
     CDS             complement(<1..710)
                     /locus_tag="VN788_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20141.1"
                     /translation="MNGTRTKILLGALAASLFFALRAIPEPGRKQMPPGPQPTGAGVG
                     TEIAKAPKATTSAPEAAQAITSETALVNVDVLAVDEDGTVLGGLKKENFRLLDNGKPQ
                     MITNFSPVTAPITIAVLMEYNSSSYNYFSAKAVDWGSLFINRLEPLDYVALVTYDLKP
                     TVRADFTRNKAEVRDTLYTLGYPNFREANLFDSLLDTLDRLERIKGRKAMLILSTGAN
                     TFSSYTLNNVLDRLRRTDV"
     gene            926..1609
                     /locus_tag="VN788_06125"
     CDS             926..1609
                     /locus_tag="VN788_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20142.1"
                     /translation="MKIFKALLVGLMILVGAAMLAAPAGADAWNKKTQLTFSHPVEVP
                     GMVLPAGTYTFKLLPTMGSRNVVQIYNEDETKLLTTILTISDYRLTPAEDTIIEFHER
                     QAGAPAALRSWFYPGDNFGREFVYPKKRAVQLAQEAQAPVPAEAVEVPATKLETVPLV
                     AVTPEAKEEPITEAFETTPPASEVAQQTPAPSTLPKTASPTPLIALVGFISIIAGFGL
                     KLLFGKNRA"
     gene            1631..3349
                     /locus_tag="VN788_06130"
     CDS             1631..3349
                     /locus_tag="VN788_06130"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU20143.1"
                     /translation="MREEYKSGRRAIEYSGAALCILFAWPIIAAISTTSAKHAYSAQG
                     PVISVTSNLVVLPVSVLDSKGEFVSGLGIENFAVFENSQSQKIAFFRQEDTPVTVGLL
                     VDHSGSMNGKLPEVAAAAEIFARSSNPEDEMFVVNFGDDISIPAFAGGPFTADPADLQ
                     KSIEDANTIGRTALYDAIAAGLEHLRAGSHERKALIIISDGGDDASRIDYREITRLAR
                     ESRAAIYAIGLIGGLFQDQNPGVLRHLAQETGGIAYFPSDVSAVSDISKQIARDLREQ
                     YTIGYEPQQNASDRTFRKIQVKVTAAGENCTCGREWGTCSIHHPRSTFRRKANVNTSI
                     QRRRETPGHQLGRRIAIAVSYMLMLIGGMALVYAGYVVNEAHRYQNSAARVFETMRSA
                     NSETHAVPSSTVSGPSADTVSTEQATPDVSVVSVTKEAPPAIVSGDVIGRMAVPRIGL
                     SVMIAEGDSNAVLRRAVGHLSSTPLPGEAGNAVLAGHRDTFFRPLRNIRSADLITIET
                     LDGLYQYKVEWVRVMPPDNVSVLAPSQTNEITLITCFPFNYVGPAPDRFVVRALEIGP
                     GNGTEP"
     gene            3500..3664
                     /locus_tag="VN788_06135"
     CDS             3500..3664
                     /locus_tag="VN788_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20144.1"
                     /translation="MMPKKKVQKRTKPPKPAAIKRVVRPRSKAQVAGAAQIHQNIFAS
                     DWREEDKAPN"
     gene            3677..4588
                     /locus_tag="VN788_06140"
     CDS             3677..4588
                     /locus_tag="VN788_06140"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXU20145.1"
                     /translation="MATAIGAVATSPRIAFKSILFPTDFSTSSERALPFAVRLARAYG
                     ATIHMLHVLPPQPMSCETPDALARTMETQLEYAQSEVRGLETGLAGISHDVNITRAAS
                     VWEAIEESLRTTAADLIILGTHGRTGAEKFLLGSVAEEVFRRSPIPVLTVGPRVNSAV
                     QEPQRFHRILFATDFSPHSLAALPYAIALAQQNRAFLIMLHVMRAPEWHGGEAALGDS
                     FEADTNALAKIVPEDAQLWCCPLTVLAYGKPADRILQIAREDQVDLIVLGLRHSIGNI
                     GAATHLGHAVSHLVVSRAACPVLTVRA"
BASE COUNT         1069 a         1298 c         1338 g         1056 t
ORIGIN      
        1 acgtctgtcc ggcgaagccg gtcaagcacg ttgttcaatg tgtacgagct gaacgtattg
       61 gcgccagtgc tcaggatgag catcgctttg cgccctttaa tcctctcgag gcgatccaat
      121 gtatcgagga gagaatcgaa aagattcgct tcgcgaaagt tcggataacc cagcgtgtac
      181 agcgtgtcgc gcacttccgc tttgttgcgc gtgaagtcgg cgcgaacggt cggctttagg
      241 tcgtaggtca cgagcgcgac gtagtccagc ggctcaaggc gattgataaa tagggatccc
      301 caatcgactg ctttcgcgga aaaatagtta tatgaacttg agttgtattc catcagtacg
      361 gcgattgtga tcggcgcagt gaccggcgag aagttcgtga tcatttgggg tttgccgttg
      421 tcgagaagtc ggaagttctc cttcttcaac ccgccgagta cagtaccgtc ctcgtctacg
      481 gcaaggacat ccacgttcac cagcgccgtc tcgcttgtaa tcgcctgcgc cgcttcgggc
      541 gcggatgtag tcgctttcgg ggccttcgcg atttcggtcc ctactccggc accagtcggc
      601 tgcgggcccg gtggcatttg cttgcgtccc ggttccggaa tcgccctaag tgcaaaaaag
      661 aggctcgccg ccaatgcccc caataatatt tttgtccttg tgccattcat aacgaacctg
      721 ctttcgtcgg cctttgtgtg ggcgcccacg aacaactgtc aaagttgata gctcaaacaa
      781 agaaacctct gctgtgacgc ccgtcacgcc cagaggtgtg acgcaacgca cagcgtcgga
      841 attggacact gaggcacaaa taatcagagc gggcggggaa tgcagcttta cgagactctg
      901 agcccgcctc gaaaggagaa ggttgatgaa aattttcaag gcgttgctgg tcgggctgat
      961 gattcttgtt ggcgctgcta tgctggccgc gcccgcagga gcggatgcct ggaacaagaa
     1021 aactcagttg acatttagcc accccgtcga ggtgcctggg atggtattgc cggctggaac
     1081 ctatacattc aagctgctgc ccacgatggg atctagaaac gtggtgcaga tttacaatga
     1141 agacgaaacc aaactgctca cgacaatcct taccatttcc gactaccggc tgacgcccgc
     1201 cgaggacaca attattgaat ttcacgagcg ccaggcggga gcacctgccg ctctccgctc
     1261 atggttttat ccgggagata actttgggcg ggaatttgtg tatccgaaga agcgggccgt
     1321 ccaattagct caggaagcac aggcgcctgt cccggcagaa gctgtcgaag ttccagcgac
     1381 taagcttgag acagtgcctc ttgttgcagt tactcctgag gccaaggaag agccaataac
     1441 cgaggcgttt gaaacgacac cgccggcaag tgaggttgca caacaaacgc cggcgccttc
     1501 gactctgcca aagacagcaa gcccaacgcc gttgatcgcg ctggtcggat tcatttcgat
     1561 catcgcggga tttggcctga agctgctgtt tggcaaaaat cgggcatgag cgttgacggg
     1621 acttcagccc atgcgtgaag aatacaaatc agggcggcgc gcaatcgaat atagtggcgc
     1681 cgccctgtgc attttgttcg cgtggccgat catagcagcg ataagcacaa caagcgcgaa
     1741 acacgcgtac agtgctcaag gtccagtcat ttccgtcacg tccaatctcg tagttctgcc
     1801 cgtgagtgtg ctcgattcca aaggtgagtt cgtctccggc ttgggaatcg agaattttgc
     1861 ggttttcgag aatagtcagt cgcagaaaat agcgttcttt cggcaagaag atacgccggt
     1921 aaccgttggt ttattggttg accacagcgg aagcatgaat ggaaagttgc ccgaagttgc
     1981 tgccgcggct gaaatcttcg cgcgctccag caatccagag gacgaaatgt tcgtcgtgaa
     2041 tttcggcgac gacatatcca ttcctgcctt tgcaggcgga ccgttcacgg ccgatccggc
     2101 ggacctgcag aagtccattg aagatgccaa cacaatcggc cgaactgctc tttatgacgc
     2161 aatcgctgct ggactggagc atctgcgagc tggaagccac gaacgcaagg ccctcatcat
     2221 catcagcgat ggaggcgacg atgcgagccg catcgactat cgcgaaatta cacgcttggc
     2281 ccgcgagtca agagctgcaa tttacgctat cgggctaatc ggtggtcttt ttcaggatca
     2341 gaatccgggc gtattgcgtc accttgcgca ggagacgggc ggcattgcgt attttccttc
     2401 cgatgtctct gccgtgagcg acatatcgaa acagatcgca cgcgatctgc gcgaacaata
     2461 caccatcggc tacgagccac aacagaatgc aagtgataga acttttcgca agattcaggt
     2521 gaaggtcaca gccgcgggcg aaaattgcac gtgcggacgc gagtggggta cctgttccat
     2581 ccatcacccg cgatctacgt tccggagaaa agctaacgtg aatacgtcga tccagcggcg
     2641 acgagaaacg cctggacatc aactcggtcg aaggatcgcg attgcggttt cttatatgct
     2701 catgctgatt ggcgggatgg cgcttgtgta cgctggctac gttgttaatg aggctcatcg
     2761 ctatcagaac agtgctgcgc gggtgtttga aacaatgcga tcggctaact ccgaaacgca
     2821 tgcggtgccg tcttcgacgg tttcgggtcc gtctgccgat actgtttcga ctgagcaggc
     2881 gacgcccgac gtttcggttg tttccgtcac aaaagaggct cctccggcta tcgtcagtgg
     2941 cgacgttatt gggcgcatgg ccgtgccccg catcggtttg agcgtgatga tcgcggaagg
     3001 cgattcgaat gctgtgcttc gccgcgctgt cgggcatttg agttccacgc cccttccggg
     3061 cgaggccggt aatgctgtgc ttgcgggcca ccgcgacaca ttcttccggc ccctgcgaaa
     3121 catccggtca gccgacctca tcacgatcga gaccttggat ggcctgtatc aatacaaagt
     3181 cgaatgggtt cgcgtgatgc cgccagacaa cgtaagcgtt cttgctccat cccaaaccaa
     3241 cgagattacc ctcatcactt gcttcccatt caattatgtc ggtccagcgc ctgaccgctt
     3301 cgtagtaaga gcgttagaga tcgggccagg aaacgggacc gagccttaag tacatcaagc
     3361 ctgtccaata gtcccgccgc tcttaagcct ttctagccct ccgaggacgt gtgacgtatg
     3421 tcaccgcgcg tgaacggcag tccagcgatc ctatggccgg ggagggttat tcggaatcgt
     3481 ctcgcaggag gtaatcagca tgatgccgaa aaagaaagtc caaaaacgca cgaagccgcc
     3541 caagccggcc gcgatcaagc gcgttgttcg acctcggtct aaggcgcaag tcgcgggagc
     3601 cgctcagatt catcagaata tttttgcgtc agattggcgc gaagaagaca aggccccgaa
     3661 ttagtgggag gatcaaatgg ccacggctat tggagcagtc gcaacgagcc cgcgcatagc
     3721 cttcaagagc attctgtttc caacggactt ttctacatca tctgaacgag cgctgccgtt
     3781 tgccgtaagg ttggcgcgtg catatggcgc gacgattcat atgcttcatg tgcttccgcc
     3841 gcaaccgatg agctgtgaaa ctcccgatgc actcgcccgc acgatggaga ctcagttgga
     3901 atatgcccaa tccgaagtgc ggggtctgga aacagggctt gcgggaatat cacatgacgt
     3961 gaatattacc cgtgcggcga gcgtgtggga ggcgattgag gagtcgctac gcactaccgc
     4021 cgcagatctc attattttgg gtactcacgg tcgtacaggc gccgaaaaat tcctgcttgg
     4081 ctcggttgcc gaagaagtgt tcaggcgctc gcccattcca gtgcttacgg ttggcccccg
     4141 cgtaaactct gcggtccagg aaccacaacg tttccaccgt attctttttg ccacagactt
     4201 ttcgccgcat tcgctggcgg ccttgccgta cgcaatcgcg ttggctcagc aaaaccgtgc
     4261 attcctcatc atgcttcacg tgatgcgggc acccgaatgg catggtggag aagccgctct
     4321 cggagattcc ttcgaagcag acacaaacgc gctcgcgaaa atagtcccgg aagatgcgca
     4381 actctggtgc tgtccactga cggtactcgc atacggaaaa cctgcggatc gaatcctgca
     4441 aatcgcacgt gaagaccagg ttgacttgat tgtgctcggt ttgcggcaca gcattggcaa
     4501 tatcggcgcc gctacgcatc tcggccatgc cgtctctcat ctggtagtgt cgcgagctgc
     4561 ctgcccagtc ttaaccgtgc gagcctgact tcgtagaacg agaacattat tcgaaggagg
     4621 aacacatgaa agtcgtcgag ctaatgacag gaactccagc ctattgccgt cccgatacga
     4681 atctggggga agccgttgag ttgatgtgga attacaattg tggaatcctg cccctcgtcg
     4741 acgctgacga gaaagttgtc g
//
LOCUS       DATNDT010000188         3320 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3459535, whole genome shotgun sequence.
ACCESSION   DATNDT010000188 DATNDT010000000
VERSION     DATNDT010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3320
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3459535"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1291
                     /locus_tag="VN788_06145"
     CDS             <1..1291
                     /locus_tag="VN788_06145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20146.1"
                     /translation="GSSLYEIEPDGEPRTIWTDAHALVYAIAIGHDGRPILGTGNHGT
                     LFRIDSPTLNTRLADLAPTQATAFCPGPDGKLYVATGNIGKVYGVGPALSRAGTFESE
                     VLDAKSFSYWGRLSFLALGREGHVAFFTRSGNLNHPRDNWSPWSQVSVEGDGGNITSP
                     PARFLQYKIEMTAGTVSPEISEVDLAYLEKNAAPQVERIEITPPNYRFPEQTLMLTPS
                     RNITLPPIGAPEKRGGRPGAPAIGTAQTLSYAKGFLGARWQAMDPNDDTLTYKVEIRE
                     AGEKMWRPLKDKVREKELSWDSTAFPDGQYELRVTASDEPSNPPAQSLTGALISDTFR
                     VDNTPPLIEDLSAAKNGRTVTVRFKTSDALSVIDKAEYSIDGTEWKLVEPAGRLSDSR
                     SENYDLTLDSVAPGEHVIAVRVTDEYENQAVRQALVH"
     gene            complement(1357..2064)
                     /locus_tag="VN788_06150"
     CDS             complement(1357..2064)
                     /locus_tag="VN788_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013358.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR1/FUN34/YaaH family transporter"
                     /protein_id="HXU20147.1"
                     /translation="MPYESMQQPVAPDTPHRIYLQPIAAPSILGLYALAGGVFVVSTN
                     MAHWYGSSETMLLLIPFVVFFGGLAQFLAGMWAYKARDGLATVLHGMWGSFFMGYSLL
                     YLVFEFGRTAVPVPTTALFPDIGVCFIVLAAITWVIAGAAAAESRSLTSTMVLLAAGA
                     TLAAIGLLAGADALTVIAGWVLFVSSLFAWYTASALLLEDACGREIWGLGKSKRMKEA
                     PQVMTGIGEPGVIHGQA"
     gene            2136..>3320
                     /locus_tag="VN788_06155"
     CDS             2136..>3320
                     /locus_tag="VN788_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HXU20148.1"
                     /translation="MPTLPLPSYIAKSTPNPPSNRAAWFANTAPSYAGVFLWIAFYQS
                     IAEGTIDKAGLGICVLALLVAGLLSYGLYYYVPAMLGMKTGYPLYVVGSSTFGTAGGY
                     LMPGLLMGLLQIGWFAVGTFLSTQFILKGLHMDSRAGTLPFAVIAVIWTYVMAYIGVK
                     GIQYVAKVSLFLNIIPLLMILFVFVKTAPGMSKHVPSHPDPFGAFTVLIAIVIGFFAT
                     AGAAGADFGMNNRNASDVKWGGLVGITLPILIAGGLPLLSVAGARALDPSLQSYQYGD
                     VIGFIGGWIGPAMFFLFALASVTPACFCAFIAGNSFSTMIPSVPRVSSTMVGVTIAAI
                     LAITGAAANLITFFNIVGASFGPICGAMAADYLLSGRKWAGPRRGINWAGYIAWALGF
                     LVG"
BASE COUNT          698 a          963 c          961 g          698 t
ORIGIN      
        1 aggatcgagc ctgtatgaaa tcgagccgga cggcgagcct cgcacgatct ggacggacgc
       61 ccatgctctg gtttacgcga tcgcgatcgg ccacgacggc cggccaatac tcggcacggg
      121 aaatcacggt acgctattcc gtatcgattc tccgaccctc aacaccaggc tcgcggatct
      181 tgcgccaaca caggccaccg cgttctgccc gggaccggat ggaaagctat acgtagcgac
      241 cggcaatatc ggcaaagtgt acggcgtggg tcccgctctt tctcgcgcgg gtacgtttga
      301 gagcgaagtg ctagacgcga aatcgttctc gtattggggg cggctttcat ttctggccct
      361 gggacgtgag ggccacgtgg cattcttcac gcgcagcgga aacctgaacc atccacgcga
      421 taactggagt ccctggtccc aagtgagtgt cgagggggac ggcggaaaca tcacttcgcc
      481 gcccgcgcgc ttcctgcagt acaaaatcga gatgaccgcc gggacagttt cgccggaaat
      541 ttcggaggtg gatttagcct atctcgaaaa gaacgccgcg ccgcaagtgg aacgcatcga
      601 gatcacgccc ccgaattatc gctttcccga acaaaccctg atgctgacgc cctcccgcaa
      661 catcacgttg ccgccgatcg gcgccccgga gaagcgcgga gggcgccctg gcgcgccggc
      721 aataggaacc gcgcaaacct tgagttatgc gaaaggattc ctaggcgcgc gctggcaggc
      781 aatggatccg aacgacgaca cgcttacgta caaggtcgag attcgtgagg caggagagaa
      841 gatgtggcgg ccgttgaagg ataaggttcg cgagaaggaa ctcagttggg attccaccgc
      901 gttccccgac ggtcaatatg agctgcgcgt gacggcgtcc gacgagccat cgaatccgcc
      961 cgcgcagtct ttgaccggcg cgttaatcag cgatacgttt cgcgtcgata atacaccacc
     1021 cctcatcgag gatttatcgg cagcaaaaaa cggacgaacc gttaccgttc gcttcaaaac
     1081 cagcgatgcg ctcagcgtga tcgataaggc ggaatattca atcgacggga ccgagtggaa
     1141 actcgtcgag ccagccggac gcctctccga ttcgcgaagt gaaaactacg atttgaccct
     1201 agactcagtc gcaccgggcg agcacgtcat cgcggtgcga gtcaccgatg agtacgagaa
     1261 ccaagcggtc cgccaggcgc ttgtgcatta gcgatgtgtg attatttggg accacggcag
     1321 tttttttcct tggtcccaaa tggcggcgtt caggctttaa gcttggccgt ggatgacgcc
     1381 gggctcgcca attccagtca tcacttgcgg agcttctttc atgcgcttgc tcttcccaag
     1441 tccccaaatt tcgcggccgc acgcgtcttc aagaagcagc gcgctggcgg tgtaccatgc
     1501 gaacagagaa gatacaaaca gcacccagcc ggcaataacg gtcaaggcgt cggcgccagc
     1561 tagtaatccg atagccgcta gggtagctcc cgcggccagc aggaccatcg tgctggtgag
     1621 cgaccggctt tcagcggcgg cggcgcccgc gattacccaa gtgattgcgg cgagtacgat
     1681 gaagcacacg ccgatatcgg gaaacagagc cgtcgtgggt acgggaaccg cggttctccc
     1741 aaattcaaaa acaagataca acaggctata gcccataaag aacgatcccc acatgccgtg
     1801 gagcaccgtc gccagcccat ctctcgcttt ataggcccac attccggcca ggaattgggc
     1861 caagccaccg aaaaacacaa cgaacggaat gagcaacaac atggtttccg aactgccgta
     1921 ccaatgcgcc atgttagtgg aaacgacaaa gactccgcca gctaaggcat agagtccaag
     1981 gatggatgga gcggcaatcg gctgcagata gatacggtgc ggcgtatcgg gggcaaccgg
     2041 ttgctgcatt gactcgtaag gcataattgg tgccctcctt acctactttc attttaccgc
     2101 cggagttccc gcgcgatgat gtataatccg ctccaatgcc cacgttaccg ttaccgagct
     2161 acatcgccaa gagcacgccg aatccgccgt ccaatcgcgc ggcctggttt gccaacaccg
     2221 cgccgagcta cgcgggagtc ttcctctgga ttgcgttcta tcagtcaatc gcggagggga
     2281 cgatcgacaa agccggtttg gggatctgcg tgttggcttt gttggtcgcc gggttactca
     2341 gctatggcct ctactactac gtgcccgcaa tgctgggcat gaagaccggc tatcccctgt
     2401 acgtcgtggg aagctccact ttcggtactg ctggcggcta tttaatgcca gggttactga
     2461 tggggctgct acaaatcggc tggttcgctg ttggaacttt tctttcaacc cagttcatct
     2521 tgaagggcct gcacatggat tccagggcgg gcacgctgcc ctttgcggtc atcgccgtga
     2581 tctggaccta cgtaatggca tatatcggcg tgaaaggtat ccaatacgtc gcgaaagtat
     2641 cgctattcct gaacatcatt ccgctgttga tgatcctgtt cgtgttcgtg aagacagccc
     2701 ctgggatgtc gaaacacgtc ccatctcatc ccgatccctt cggtgcattc acggttctca
     2761 tcgcgatcgt gatcggtttc ttcgccacgg cgggcgcggc gggcgcggat ttcggaatga
     2821 ataaccggaa tgcaagcgac gtgaagtggg gcggcttagt cggcatcact ctgccgatat
     2881 tgatcgcagg tggccttcct ttgctttccg tcgccggcgc tcgcgcgctt gatccgagtc
     2941 ttcagagcta tcaatacggg gatgtcatcg gcttcatcgg gggttggatc ggtcccgcga
     3001 tgttctttct gtttgcgctc gcttccgtaa ctccggcctg cttctgtgcc ttcatcgcgg
     3061 ggaatagctt ttcgacgatg atccccagcg tgccgcgcgt ctcatccacg atggtcggcg
     3121 ttacgatcgc cgcgatttta gcgatcaccg gagccgctgc gaatctgatc accttcttca
     3181 acattgtcgg cgcgtccttt ggaccgatct gcggggcaat ggcggcagat tacctactat
     3241 ccggaagaaa gtgggcgggc cctcgccggg gaatcaattg ggcgggttat atcgcgtggg
     3301 cgcttggatt tctggtcggc
//
LOCUS       DATNDT010000189        11410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3656316, whole genome shotgun sequence.
ACCESSION   DATNDT010000189 DATNDT010000000
VERSION     DATNDT010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11410
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3656316"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1404)
                     /locus_tag="VN788_06160"
     CDS             complement(37..1404)
                     /locus_tag="VN788_06160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016836888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HXU20149.1"
                     /translation="MANTVYIVSALRTPIGKFGGMLADFTHPDLGTIAVRAALERAFG
                     EPLPSERTPGEFEYPPATGAATSKATNGVSNLRWHVDELIFGNARPAGVGPNPARQIA
                     WRSGLGDDVPAFTVNMACASGLRAVVLGWQEIITGQAGIIVAGGAESMSRVPYLVEGR
                     WGFRLGNQQLIDGMYRDGFLCPLSKMIMGETAEMLADQYKISREEQDEFAVESHRRAT
                     VASAECHFKEEIVPVPVTDKKGNVTILLKDEHVRKDASLVGMAKLAPVFSKPGIVVDK
                     KGDITILPAFSNPGTVTAGNSSGITDGAAAVVLASEEKVRELRLKPIARIVDASIAAV
                     DPRVMGIGPVPAVRNLTKRTGTKLEDYDAIELNEAFAAQVLACDHDLHFNRERLNPNG
                     GAIALGHPIACTGSRILTTLIHEVRRPSGPGTGPSASPRAGRRGLATLCVSGGLGMAV
                     EIETV"
     gene            1725..2099
                     /locus_tag="VN788_06165"
     CDS             1725..2099
                     /locus_tag="VN788_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20150.1"
                     /translation="MSDKPKIRDDSERELSHQLRTVSPIEKARENTTSSVQTADVEQR
                     LDALRQRVRNLKADSSVVWLEDRLHIFDPALDLRVKVEAVHLMLRAAELLSDSDKEKV
                     LLTVRNSLEQLEKAVELDVHAA"
     gene            2192..3202
                     /locus_tag="VN788_06170"
     CDS             2192..3202
                     /locus_tag="VN788_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016859293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HXU20151.1"
                     /translation="MNVYEIPKDVSKLEDLRRAQRADPTPGPYDVLVRIRAAALNYRD
                     HAIIVGSYRYNLDRATIPCSDGAGDVVAVGSNVTRFKPGDRVIPSFFQVWIDGVPPKN
                     RAALGAPLDGTLAEYIALHEDGWVAMPKSLSYEEAATLPCAGATAWNALMLSGSPIKP
                     GDTVLCLGTGGVAMFALQFARAAGARVIVTSSSDAKIDRARVLGAFGGVNYKTHPDWE
                     KEVLALTQGRGVDCVIENGGIGTLARSFQCVGWKGKIALIGVLAGREGNANPHELMFK
                     SASLHGIGVGSRACLEQLIQAIEVNRIKPVIEKVFPFDHALEAFRLQASGNFLGKIAI
                     AI"
     gene            3282..4547
                     /locus_tag="VN788_06175"
     CDS             3282..4547
                     /locus_tag="VN788_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HXU20152.1"
                     /translation="MPRTRRGDKHPLRPSTKVLTEGFDPTLSLGSARPAVFRSSTYVF
                     SSPEAAEHAFNVTSGRARLPEGEGPDLIYSRFNHPNAQMLEDQIVNLEKGSKAALAFN
                     CGMAAIVTSLFALLRPGQSIVYTVPIYGGTQHFIQDCLSQWGISGTPVFSGDAGRISR
                     AIASAKNLGAVMLETPANPTLIMTDIRQVCDAVAALKIPKGRARPLVLVDNTMLGPSF
                     QHPLELGADLVLYSGTKYLSGFSDMLAGIALAKDLGLIRRIRPTRGLFGTILQPDECW
                     MLGGRLPTVRLRMDKSSIDAQRIAERLAQHTKVKKVHYPTLFTDPEQKRIYAAQCDYP
                     GGMVSLELRGGKHAAFDFLRHLKIARNAVSLGGVETLACHPKTTTHSGFSEKEFAQAG
                     ITDGLVRISVGIEHWRDLLADFEQALDAV"
     gene            5041..5994
                     /locus_tag="VN788_06180"
     CDS             5041..5994
                     /locus_tag="VN788_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXU20153.1"
                     /translation="MKVKDAICLGRLAAIAILFVACAAPMVRAQGTAPETAPPLFPGG
                     ALISYNSIFHARGLAPGIFPSISATAKPTLSHEGDFNFTWGFFPNFDVTVLVPVVTNH
                     FDIPGAPAVGGTGLGDAMILVKYRFYRRDSPRGTTQASITFGPKLPTGLTHLNGINGG
                     RLPATLQPGSGSTDLFLSANWTYTGLFNLRRLVADEDFHSLIRTEGTQATRLGSDIES
                     RFWLSYRPYEAKDATREWFIGPALTWLHSQDDRIAGITQKGSGGDVLLAGVTTYVGVR
                     PGMHLWLGMDWDVAHSNGAMFMPIRRHISFGITQQFRFHMF"
     gene            6210..6539
                     /locus_tag="VN788_06185"
     CDS             6210..6539
                     /locus_tag="VN788_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20154.1"
                     /translation="MQIAPKEDGQIDPAHLLKAIYDSGVTATEMDVTVEGRMIKDASG
                     ALAFQFEPNRSWVITPNDQSRSLADLAGTPSKITVTGQLYLKSGKKKANPSVPLKLLI
                     TKIQKNE"
     gene            6552..7103
                     /locus_tag="VN788_06190"
     CDS             6552..7103
                     /locus_tag="VN788_06190"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXU20155.1"
                     /translation="MWHRADRYLVNLLLVFAAVALAGCHHGSASATKPAQSAIAAGEI
                     GSRLPDFSVKDFQGREISSSDFRGKVLLVDVWATWCQPCKKEMPGYQKLLDRYGSRGF
                     AVLGLKATMMADTEDPIQFAREVGVHYPLAIASPQAMQALGGILGLPTTFLYDRQGIL
                     RYKVIGFEYTNVIEAHLKPLLGR"
     gene            7427..7663
                     /locus_tag="VN788_06195"
     CDS             7427..7663
                     /locus_tag="VN788_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019489367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HXU20156.1"
                     /translation="MEKTYCVYILASRSRNLYTGVTNNLHRRLAEHREGHIAGFTSRY
                     NIFRLVHYEVFSDIRTAIAREKEIKAWRREKKFG"
     gene            8230..9558
                     /gene="serS"
                     /locus_tag="VN788_06200"
     CDS             8230..9558
                     /gene="serS"
                     /locus_tag="VN788_06200"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009609894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HXU20157.1"
                     /translation="MLDLGFVREHMDAIEKMARDRGITLDLGPFVEVDTERRKLITAT
                     EQMKAERNKANDIIGPLQKTIRDLEAKGQDVTDRIEQRDKLLRQMKESSDKIKRSDEL
                     VAKMDDALKEFLLTIPNIPHASVPLGKSAADNVEVRRSGEPPKFGFAPRPHWELGERA
                     GILDFESAVKIAGARFAVYKGLGARLERALANFFLDVHTREHGYTEILPPFIVNTAAL
                     TGVGQLPKFSSDMFHLEGTDLWLTPTSEVELTNLYRDETIDADELPIKVCAWTACFRS
                     EAGAAGKDTRGIKRQHQFQKVELFKFTRPEKSYDELETLVRNAEKMLERLGLHYRVMQ
                     LCTADMGFASAKTYDIEVWLPSAGEFMEISSCSNTEAFQARRSGIRFRPKGGPRNQKS
                     EFVHTLNGSGLAVGRAWIAIVENFQQADGSIAIPEVLVPYMGVEKIKPKD"
     gene            complement(9639..9986)
                     /locus_tag="VN788_06205"
     CDS             complement(9639..9986)
                     /locus_tag="VN788_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20158.1"
                     /translation="MKRAISMYSVLAVMLAAISLATFAENKTMTWTGWISDSHCGAKG
                     MSADHKACAETCVKTKGASWVFVSGKSKKVLPIKNQDAVNADQDLGASVKVTGHVTDD
                     GSIQVDSIAKAKT"
     gene            complement(10186..10977)
                     /locus_tag="VN788_06210"
     CDS             complement(10186..10977)
                     /locus_tag="VN788_06210"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HXU20159.1"
                     /translation="MSSHPTCDEDFDLYALGALEGTEKQALESHVAFCLGCAQKLADA
                     RGRIAILALATPAARAPVNVKERLLEQIRAAREGGAILTELKPQPARRPGMSRWTAIL
                     LPIAAVLAIAAFLLWNANRRLDIQLTALRASMQQEQRQLQDARDIASLITARDTITVQ
                     MAPQQAAMPHGTAHVMYNAKMGMMMYDGQIPPAPAAKSYQLWLVPASGKPISCGVFNP
                     VEGQPDHWMTKLPKGIQPKSFAVTLEPSGGMPQPTGPKVLVGAPS"
     gene            complement(10988..>11410)
                     /locus_tag="VN788_06215"
     CDS             complement(10988..>11410)
                     /locus_tag="VN788_06215"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU20160.1"
                     /translation="AVEEILQDIFFQLWNTASRFDSSRGSLPAWLVVMARNRAISRLR
                     RRNPAAGDTPVEGLATSSFNLESSAARQQLVERVKASLEKLPKEQRTAVELAFFEGLT
                     HSEIAARTGDPLGTVKTRLRSALETLRRTLHSAAQSGN"
BASE COUNT         2380 a         3488 c         3130 g         2412 t
ORIGIN      
        1 actccccttg gacgcgggga cgtcttcgtc caaaggttag accgtttcga tttcgactgc
       61 catgcccaat ccgccggaca cacacaatgt agcaaggccc cgccggccag cccttggact
      121 ggccgatggg ccagttcccg gtccggacgg cctgcgcact tcgtgaatta acgtggtgag
      181 aatgcggctg ccagtgcacg cgatcggatg cccgagcgcg attgcacctc cattgggatt
      241 aagccgctcg cgattaaagt ggagatcgtg gtcgcaggcc agcacctgcg ccgcgaaggc
      301 ttcgttcagc tcgattgcat cgtaatcctc gagcttcgtt ccggtgcgct ttgtcaggtt
      361 gcgcaccgcc ggcactggac cgattcccat cacgcggggg tccaccgccg caatgctcgc
      421 gtccacgatt cgcgcaatcg gcttaagccg cagttcgcga actttttctt cgctagccag
      481 gactaccgcc gccgcgccgt cggtgatacc actggaattt cccgccgtga cggtcccggg
      541 attgctgaag gcgggcagaa tcgtgatgtc acccttcttg tccactacga tgccgggctt
      601 gctgaacacc ggcgccagct tggccatccc cacgagcgac gcatccttcc gcacatgctc
      661 gtccttcagc agaatggtca cgttgccttt tttatccgtc acgggcaccg gaacaatttc
      721 ttctttgaag tggcactcgg cggaagcaac cgtggcgcgg cggtgactct ccaccgcaaa
      781 ttcgtcctgt tcctcgcgcg aaattttgta ttggtcagcg agcatctcag ccgtttcccc
      841 cataatcatc ttcgagaggg gacagagaaa gccatcacgg tacattccgt cgatcagttg
      901 ctggttcccc agccggaatc cccatcgccc ttccactagg tatggcacgc ggctcatcga
      961 ctccgcgccg cccgcgacaa taatcccagc ctggcctgta atgatctcct gccagcccag
     1021 cactacggcg cgtagccccg aagcgcacgc catattcaca gtgaacgccg gtacgtcatc
     1081 gccaagacct gaccgccacg cgatttgtcg cgcgggattc ggccccacac ccgccggtcg
     1141 cgcattgccg aagatcaatt catccacgtg ccaacggagg ttcgagacgc cgttcgtagc
     1201 cttactagtc gccgctccgg tcgcgggcgg gtactcgaat tcgcccggcg tccgctccga
     1261 tggcagcggt tcgccgaacg cgcgttcaag cgccgcacgc acagcaatcg ttcccaggtc
     1321 tgggtgcgtg aaatccgcca gcatgccgcc gaattttccg atcggcgtac gtaacgcgct
     1381 cacgatgtaa accgtatttg ccacagttgt tcgatgttag cacagctctt tccagcttcc
     1441 atatcgcccc aattcgcggc gcaaagccat cgcggattgc tttcttacgg cctggatcgc
     1501 acggaacaaa ctgcggcaga cgggaacaat ctccacggtt cagtaccaac gaagttttgt
     1561 aaagtaaatt tcgccctggg tcgctaaatc gcggcgttaa aagcacttcg ctaaaggcag
     1621 ggggttggta ctctcattgc tcagctaatt tcgtgtctca acacgagagt aacgacaggg
     1681 ttggaacaaa tttagaatgt ctagtaacag gtagcgagtc gctgatgtca gataaaccga
     1741 aaattcgcga tgattccgag cgggagctga gtcaccagct tcggaccgtg tctccaattg
     1801 aaaaggcgcg ggaaaatacc acctcaagtg tccagacggc cgacgtggaa cagcggctgg
     1861 atgctctccg gcagcgtgtg cgcaacctaa aggccgattc ctctgtagtc tggctggaag
     1921 accgcctgca cattttcgat ccggccctgg atttgcgcgt gaaagtggag gcagtgcacc
     1981 tgatgttgcg cgctgcggaa ctgctcagcg actccgacaa agaaaaggtg ctcctaaccg
     2041 tcaggaattc cctcgagcag ctcgaaaaag ccgtcgagct cgacgtgcac gccgcctgat
     2101 tcggcagccg gccgttgcca aaacggtttc cgtccttgca tcttcattcc tcgcttctca
     2161 gcacgcgaaa attccgttat tctctcgccc catgaatgtc tacgaaattc ccaaggacgt
     2221 cagcaagctc gaagacctgc gccgcgcgca gcgcgctgat cccacgccgg gtccctatga
     2281 tgttctcgtt cgcatacgcg cggccgcgct caattaccgc gatcacgcga tcatcgtcgg
     2341 ttcctatcgc tataacctag atcgcgccac gattccctgc tccgatggcg ccggagatgt
     2401 ggtcgcggtc ggttcgaacg tcacacgttt caagccgggc gaccgcgtga tcccttcttt
     2461 cttccaagtg tggattgacg gcgtcccccc gaaaaaccgc gcggctctag gcgcgccgct
     2521 cgatggcacg ctcgcggagt acatcgctct ccacgaagat ggctgggtcg ccatgccgaa
     2581 aagtctctcg tatgaagagg cggccacgct tccttgtgca ggcgccacgg cgtggaatgc
     2641 tctgatgctc tccggctcgc ctattaagcc gggagacacc gtgctttgcc tgggaacggg
     2701 cggcgttgca atgttcgcat tgcagttcgc gcgagccgca ggagcgcgtg tcatcgtcac
     2761 ctcgtccagc gacgcgaaaa tcgatcgcgc tcgcgtcctc ggcgccttcg gcggcgtgaa
     2821 ttacaagacg catcccgatt gggaaaagga agtgctcgca ctcacccaag gacgcggcgt
     2881 cgattgcgtg atcgaaaatg gcggcattgg gacgctcgcg cgctcgtttc aatgtgtcgg
     2941 atggaaggga aaaatcgcac tgatcggcgt gctcgcagga cgcgagggca atgccaatcc
     3001 gcacgagctc atgttcaaga gcgccagcct gcacggtatc ggagtcggca gtcgcgcctg
     3061 cctcgagcaa ttgattcagg ctatcgaagt caaccgcatc aagcccgtca tcgaaaaagt
     3121 ctttcctttt gaccatgccc tcgaagcatt ccgcctccaa gcctccggca acttcctcgg
     3181 caaaatcgcc atcgccattt gaaccgcgcc gctcggccgc aggataacca tctccgatcg
     3241 cgcggcgtgt tagcttataa ctgtgaaaag gagattccgc catgccacgc acccgccgcg
     3301 gcgataagca ccctttgagg ccgtcgacga aagttctcac cgaaggattc gacccgactc
     3361 tttctctcgg ctccgcgcgt cctgctgttt ttcgcagttc cacttatgtt ttttcaagcc
     3421 cggaagccgc tgagcacgcc ttcaacgtta cctccggacg cgcacgcctg cccgaaggcg
     3481 aagggccgga cctgatttac tcgcgcttca atcatcccaa cgcccagatg ctcgaggatc
     3541 agatcgtgaa cctcgagaaa ggttccaaag ccgcgctggc cttcaattgc ggcatggccg
     3601 cgatcgtcac atcgcttttt gcgcttttgc gtccgggcca atccatcgtt tacacagtcc
     3661 cgatttacgg cggcacgcag catttcattc aagactgcct atcgcaatgg ggcatctcgg
     3721 gcactccagt tttttcgggc gacgccggtc gaatcagccg cgcgatcgcg tccgcgaaaa
     3781 atctcggcgc ggtgatgctg gaaacgcccg ccaatcccac gctgatcatg accgacatcc
     3841 gtcaggtttg cgacgccgtc gccgcgctga aaattcccaa aggtcgcgcg cgccctctgg
     3901 tgctggtaga caacactatg ctaggcccct cgttccagca tccgctggaa ctcggcgccg
     3961 atctcgtact ctattcgggg acaaaatatc tttccggctt cagcgatatg ctcgccggaa
     4021 tagcgctcgc gaaagatctc ggcctgattc gcagaattcg tcccacacgc ggcctgttcg
     4081 gcaccatcct tcagcccgac gagtgctgga tgctcggcgg acgcttgcct accgtgcgct
     4141 tgcgcatgga taaatccagc attgacgcgc agcgcatcgc cgagcgcctt gcacagcaca
     4201 cgaaagtcaa aaaagttcac taccccacac tcttcaccga tccagagcag aaaagaattt
     4261 acgccgcgca atgcgattat cccggtggca tggtctcgct cgagcttcgc ggtggaaaac
     4321 acgcggcgtt tgattttctg cgccacctga aaatcgcgag aaatgctgtc tcgctcggcg
     4381 gcgttgaaac tctcgcctgc catccaaaaa ccaccacgca ttccggcttc agcgaaaaag
     4441 aattcgcgca agccggcatc accgacggcc tcgtccgcat ctccgtcggc atcgagcact
     4501 ggcgcgacct cctcgccgac ttcgagcaag ccctcgacgc cgtctgaccg ccgctcggat
     4561 tcgcaacttg actcctcctg cagcgcggtc tagcctgtat ttaggatgcg cttgaccaag
     4621 atattcctgc tttcggccct cgcgctaagc ctgtcaagtt atgcggtcga ttgctccgcg
     4681 ctccagaccc cagcggcggc gatgcactgt tgcgcttcga tgccctgttc gcacagccac
     4741 ggccagcagg actgctgcaa gaacatgccg tccatgcacg tgccttttgt gcagcccgcc
     4801 tcgcttcaca atatacagtt tccgttgtcc atcgctgcgg cattgcccgc ggcggcgaat
     4861 tcggaggctt tagaccgatc gtggccgttc gcaacactgt attgtcacgc ccctcctatt
     4921 tttccttcgc ctggtttttc accacttcgc atctgatcgc tcctccgggt aaaagcggtt
     4981 cgacccgcta gcgcggataa gtgtttccgc gcgcggccat ctttactgga ggaaaaatcc
     5041 gtgaaagtta aagacgcgat ctgcctaggc agactcgccg caatagctat tctatttgtc
     5101 gcttgcgccg cacctatggt tcgcgcgcaa ggtactgcgc ccgaaaccgc tccgccgttg
     5161 ttccccggcg gcgccctaat ttcctacaat tccattttcc acgcgcgcgg cttggcaccc
     5221 ggcatttttc cgagcatctc tgccacggcg aagcccacgt tgtcccatga aggcgatttc
     5281 aatttcacct ggggattctt tcccaatttt gacgtgaccg ttctcgttcc cgtggttaca
     5341 aatcactttg acatccccgg cgcgccagcc gtgggcggca cggggcttgg cgacgcaatg
     5401 attctagtga aatatcgctt ctaccgccgc gattcacccc gcgggaccac ccaggcgtcc
     5461 ataacgtttg gccccaagct tccgaccggc ctcacgcatt tgaatggaat aaatggcggc
     5521 cgcctccccg caactttgca gcccggctca ggctcgaccg atttatttct atcggcgaat
     5581 tggacgtaca ccggcctttt caatcttagg cgtctcgtgg ccgacgaaga cttccactcc
     5641 ctgatacgca cggagggaac ccaggcgact cgcctcggca gcgatatcga atcccgcttt
     5701 tggctctcgt atcgcccata cgaagccaaa gacgccactc gcgagtggtt catcggcccc
     5761 gcactcacct ggctgcattc acaggacgat cgcatcgcgg gcatcactca aaaaggaagc
     5821 ggaggtgacg tcttacttgc cggagtcacc acctacgtag gagtgcgccc cggaatgcat
     5881 ctttggctcg ggatggattg ggacgtcgcc cattcaaatg gcgcgatgtt catgccgatt
     5941 cgccgccaca taagttttgg catcacgcag cagttcagat tccacatgtt ctgaaagttc
     6001 tgaaaggaga aaaaaaaatg aaaatcaata aattggcgct gcttttactg ttgggcgcgg
     6061 ctatcgcgcc tgtctcgcag gcgcaaatca agcacgtcga gatgcgcgtc gaaggaatga
     6121 cttgaaactt ttgagcctac ggtgtgcaac aacacctgca gcgccagtct ggcgtacaaa
     6181 aagcggaagt cagcctcagg gatgggacag tgcaaatagc tccaaaagaa gatgggcaaa
     6241 tcgatcccgc tcaccttcta aaagccatat acgatagcgg cgtcaccgct accgaaatgg
     6301 acgtaacagt cgaaggccgc atgatcaaag acgcttccgg cgcactcgca tttcagttcg
     6361 aacccaatcg ctcctgggta atcactccga atgaccaatc tcggagcctc gccgacctcg
     6421 cgggcacgcc gtcaaaaatc accgtcaccg gccagttgta tctaaagtcc gggaaaaaga
     6481 aagcgaaccc atcagtgccg ctaaagctgt tgatcacaaa aattcagaaa aacgagtaga
     6541 agggaaacta aatgtggcat agagccgatc gttacctcgt aaacttgctt ctcgttttcg
     6601 cggctgtggc cctcgccgga tgccatcacg gttcggcgag cgccacaaaa ccggcgcagt
     6661 ccgccatcgc ggcaggcgag atcggttctc gccttcccga tttttccgta aaagattttc
     6721 aaggccgcga aatttcgtcc tccgattttc gcggcaaggt tctcctcgtt gatgtttggg
     6781 ccacctggtg ccagccttgc aaaaaagaaa tgccgggtta tcagaagcta ttggatcgct
     6841 atggctctcg cggtttcgca gtcctcggcc taaaggccac catgatggcg gacaccgagg
     6901 atcccattca gtttgcccgc gaagtcggag tccattaccc gttggcgatc gcgtcgccgc
     6961 aagccatgca ggcgctcggc ggaattttgg gtttacccac cacatttctc tacgaccgcc
     7021 aaggaatcct ccgctacaaa gtaatcggct tcgaatacac caacgtcatc gaagcccacc
     7081 taaaacccct cctcggtcgg tgaagcatcg attttgctgt tgcctctacg ttgtcatccc
     7141 gaaccaagcc gcgtcctttg cggattggcg tgagggatct gcttttgcca ttgtctctat
     7201 gctgtcattc cgaacgacgc tttttcgtgg tgggattctg cttctttttg cggagcgagc
     7261 tatgtgggct cgcaggcgtc gcctctgaat tggccgttgc cgttgcgttg tcatctcgaa
     7321 tccagccgcg cctttggcgg ctgtgtgcgg gatctgcttt tgcagttgtc cgtctccgcc
     7381 tctcttccct ttaggcctat attgctctcg ctccagatcc cgcaaaatgg agaagaccta
     7441 ctgcgtctac attctcgcca gtcgttcccg aaacctttac actggcgtga ccaacaacct
     7501 gcaccgaagg ctggccgagc atcgtgaagg acatatcgca ggattcacgt cgaggtacaa
     7561 cattttccgc ttggtgcatt atgaagtgtt cagcgacatt cgcaccgcaa ttgctcgcga
     7621 aaaagaaatc aaggcgtggc gacgcgaaaa gaagttcggc taatccagaa tcgcaatccc
     7681 gtatgggatg atctcgccgc agaatggttc ggaagtcagc ggaagcatca gtcgtcgtcc
     7741 cgcttaccct gacgtaggcc ggatgcagca aatagccgct ccgacgctat cgccgattcg
     7801 gcgcagttga accactcctc gcggagaaag tccacaggcg ctcgtccttg tctcgcaacc
     7861 tctcatctat aaaatggacg catggtccaa acgaccgcag gtcgggcgca gtcgtcgggt
     7921 ctcgcgagag cagggagacg tctgcgccgc cggcctggaa cctatggccc gaaaacacgg
     7981 acttccactg cgagagcgac cgcgcaaaag cacctggcta atcgacacta tggtcaagta
     8041 gaacatggtg caagtgattg caaacaaagg gtgggcgtac aatctacccg tcacaaaatc
     8101 ggaaccctcg attccagcaa tgcctgccgc cccgcacgaa cgaatgggcg ccccccacgg
     8161 cgggagccga cacgcccaac cactagccac tcgccactag ccactgcttt cggttattct
     8221 gcctccccta tgctggacct tggattcgta agagagcaca tggacgcgat cgaaaaaatg
     8281 gcccgcgatc gcggcatcac gctagacctc ggccctttcg tcgaagtgga tacagagcgg
     8341 cgcaagctga tcaccgccac cgagcaaatg aaggcggagc gcaacaaggc caacgacatc
     8401 atcggcccgc tgcagaaaac gattcgcgat ctcgaagcga agggccagga cgtgacggat
     8461 cgcatcgagc aaagggacaa gctcctgcgc cagatgaaag aatcatccga caaaatcaag
     8521 cgcagcgatg agttagtcgc caaaatggac gacgctctga aggaattctt gctgacaatt
     8581 ccgaatattc cgcacgcgag cgtccccttg ggaaaaagcg cggcggataa tgtggaggtg
     8641 cggcgctcgg gcgagccgcc gaaattcggt ttcgcgccgc gtccgcactg ggaactgggc
     8701 gagcgcgctg gaattttgga cttcgagtcc gcggtgaaaa ttgcgggcgc gcgcttcgcg
     8761 gtttacaaag gattgggagc gcgccttgag cgcgccctcg ccaatttctt tctggacgtg
     8821 cacacgcgcg aacacggcta cacggaaatt ttgccgccgt tcatcgtcaa cacggccgcg
     8881 ctcaccggag tcggccagct tccgaaattt tcctccgata tgtttcacct cgaaggcacg
     8941 gatttgtggc ttacgccgac atccgaagtg gagctcacga atctctatcg cgacgagacc
     9001 atcgacgcgg acgaattgcc catcaaagtg tgtgcgtgga cggcctgctt ccgctccgaa
     9061 gctggcgcgg cgggaaaaga cacgcgtggc atcaagcgcc agcatcaatt ccagaaagtg
     9121 gagctattca agttcacgcg gccggaaaaa agctacgacg aattggaaac gctggtccgc
     9181 aacgccgaaa agatgctcga acgcctcggc ctgcactatc gggtgatgca gctctgcacc
     9241 gcggacatgg gattcgcgtc ggctaaaacc tacgacattg aagtgtggct gccgtcggcg
     9301 ggcgagttca tggaaatttc atcgtgctcg aatacggagg cgtttcaggc acgtcgctca
     9361 gggattcggt tcaggcccaa gggtggccca agaaatcaaa agtccgaatt cgtccacacg
     9421 ctaaacggct cggggctggc ggttggacgc gcgtggatcg cgatcgtcga aaattttcag
     9481 caggccgatg gctcgatcgc aattccagaa gtgttggtcc cctacatggg agtggagaaa
     9541 atcaagccga aggattagcg agcaatcgca gaaaaaagcg cggagcgcag gtcccaaacc
     9601 cttcacctgc gcgccgcgcc cttgaaatgc gaaaaatttt acgtcttggc tttagcgatg
     9661 ctgtccacct ggatggagcc gtcatcagtt acgtggccag tgacttttac tgacgcaccc
     9721 agatcttgat ccgcgttcac tgcatcttga ttcttgatcg gtagcacttt cttggacttc
     9781 ccgctgacga aaacccagga agcgcccttc gtcttgacac aggtttcggc gcaggctttg
     9841 tggtccgcgc tcataccttt tgcgccgcag tggctgtcgc tgatccagcc agtccacgtc
     9901 atcgtcttgt tttcggcgaa ggtggcgagg ctgatcgcgg cgagcatcac tgcaagcacg
     9961 ctgtacatgc tgatggctct tttcatgttc acttcctcct caggactagc gcggcctccc
    10021 ggcgaatcat gacgaccagc aggccgatct ttcattcttc aggtacgcta cgtatacgaa
    10081 aagtggattg gttgaatcgg taaaaaaccg ttcgatgttt ctggccggac cacggaatta
    10141 cttatactcc aaggacttgc gaccggcaag aaagattgtc cggcgctagg agggtgcgcc
    10201 gactaggact tttgggccgg tgggctgcgg cattccgccg gaaggttcga gcgtgaccgc
    10261 aaaagacttg ggctggattc ctttgggcag cttcgtcatc cagtgatcgg gctgcccctc
    10321 gacgggatta aaaacgccgc agctaatcgg tttgccactc gcgggaacca gccagagttg
    10381 gtaactcttg gcagcgggag cggggggaat ctgaccgtcg tacatcatca tgcccatttt
    10441 ggcgttgtac atcacgtgag cggtgccgtg cggcatggcg gcctgctggg gagccatctg
    10501 cactgtaatc gtgtcgcggg cggtgatgag gctggcgata tcgcgggcgt cttgcagttg
    10561 ccgctgctct tgctgcatgc tggcgcgcag cgcagtgagt tgaatatcca ggcggcgatt
    10621 ggcgttccag agtaagaatg ctgcgatggc cagaacggca gcgattggga gcagaatggc
    10681 ggtccaccga gacatgcctg ggcggcgggc gggctgcggc ttcagttcgg taagaatggc
    10741 accgccctcc cgggctgcgc gaatttgctc gagcaggcgt tcctttacgt tgacgggcgc
    10801 cctcgcggcg ggcgttgcca acgcaaggat ggcgatgcgt cctcgcgcgt cggcgagctt
    10861 ctgcgcgcat ccgaggcaaa aggccacgtg cgattccagc gcctgttttt cggtgccttc
    10921 gagcgcgccg agggcgtaga gatcgaaatc ttcgtcgcaa gttggatggc tgctcatgtc
    10981 ccttagttca attccccgat tgtgccgccg aatgcaatgt gcgcctcagt gtttccagtg
    11041 cggaccgcag ccgcgtttta actgtgccca gtggatcgcc ggtgcgagcc gcaatttcgc
    11101 tgtgcgtcag tccctcgaaa aatgcaagct cgacagcggt ccgctgctct ttcggtagct
    11161 tttcaagaga ggctttcacg cgttcgacca attgctgccg tgccgccgaa ctttcgagat
    11221 tgaatgacga agtagccaaa ccttctaccg gtgtgtcgcc cgccgccgga ttgcgccgcc
    11281 gcagcctgga aatcgcgcga ttgcgagcca tcacgaccag ccatgcgggc agcgacccgc
    11341 gcgacgaatc aaagcgcgaa gcagtgttcc agagctggaa gaaaatatcc tgcaaaatct
    11401 cctcgaccgc
//
LOCUS       DATNDT010000190         4224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_223939, whole genome shotgun sequence.
ACCESSION   DATNDT010000190 DATNDT010000000
VERSION     DATNDT010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4224
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_223939"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1092)
                     /gene="hpnH"
                     /locus_tag="VN788_06220"
     CDS             complement(<1..1092)
                     /gene="hpnH"
                     /locus_tag="VN788_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HXU20161.1"
                     /translation="MAVPGPVSQMWAVSSYVVRQKLKGRKQYPLVLMLEPLLRCNLAC
                     AGCGKIQYPAHVLKKNLTPEECFRAVDECGAPVVSIPGGEPLMHPQIAEIVNGLVSRK
                     KYVYLCTNALLLSERLGEFTPSKYLSFSVHMDGQREHHDFSVCREGTYDKAVEGIRMA
                     LARGFRVTTNTTLFEGADPQSVRQFFDDMMELGVEGMMLSPGYTYDKAPDQEHFLSRK
                     KTRNLFRQILSNRSDEWRFNLSPLFLEFLMGKRDYKCTPWGMPTYNIFGWQKPCYLLQ
                     DGYADTFEELIRETNWDQYGTESGNPKCANCMVHSGYEASAVNDTFSSVGGFLSTVRA
                     TIFNKYKDPDLMGGASGNSGESSGQLIQIM"
     gene            complement(1289..2134)
                     /locus_tag="VN788_06225"
     CDS             complement(1289..2134)
                     /locus_tag="VN788_06225"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU20162.1"
                     /translation="MKRLALFAILVTFFAVGFSRDCRAQQNTSDSVQPAADHLHGAQV
                     ENANPPMTPRQTAEMRAAILMARKEFSDAAIAYQHLLETDPHNADFLNRTGMAYQELG
                     QDRLAEHFYKKAVHADKKFSQAINNLGTVEYSRKRYGKAIKYYKKALTVAPETAAAYS
                     NLGYAYCAIKKYPEAIQAFGKAVALDPMVFEHRGSSGSVLQQRTAADSGDLYFTIAKS
                     YAKLGDAERVARYLKLARDDGYKKMLEVEKDPAFAKVIKDARVQEVLRLHSTYAGEPS
                     KAESN"
     gene            complement(2179..>4224)
                     /locus_tag="VN788_06230"
     CDS             complement(2179..>4224)
                     /locus_tag="VN788_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HXU20163.1"
                     /translation="AEDKRFFEHGALDMVRVFGAAFYDLSQNRKAQGASTIDMQVART
                     FFFTTKREWRRKLKEVLMAAELDERFSKQQIFELYANDIYLGNRGSFSIRGFGEAAQA
                     YFGKDVRDLTAGEAAFLAGIIRAPNRYSSAERHVDRADEVRDRVLGEMAGDRYITADE
                     QAAAKKTKLKFVSGGVESSPAPYFVDMVKDHLLEKFSESDLESQSYRIYTTLDPDLQR
                     AASEAVQIGVKNLDKLLARRYAIWKKKGQAVPPAQVAVVVMDPHTGEIRALIGGRNYG
                     ESQLNRALSRRQPGSVFKPFVYAAAFDDAVENLEPVVTPVTTIDDSPTTFEFDGKDYT
                     PNNYGQEFYGTVTVRDALIHSLNVATVKVAELIGYQRVVDLARQMGLGGNIQATPAVA
                     LGAYDMTPIEVAAGYTAFAANGVRADPLFIRSVIAANGSTAELNAPSTRAVLDPRVAF
                     LTTTLMEDVINHGTGYPVRQIGFTAPAAGKTGTSHDGWFAGYTSNLLCVVWVGFDDNR
                     DLGLAGGQAAAPIWAEFMKRAVALPRYRDTQNFEPPPGIVAQSVDPQTGQLATPSCPK
                     IMTEYFISGTEPNQYCQLHGGSLAQSGPASWLGHLFGKGSEAAGPRPPETHEAPPNAT
                     KNGHVAIPPPAKSAQEAAPQGQTKQPEKKKGLFDKIFGIFGGEKQPEEKQPDTSKPH"
BASE COUNT          818 a         1312 c         1122 g          972 t
ORIGIN      
        1 catgatttga atcagttggc cgctgctctc gcctgaattg ccggaggcgc cccccatcag
       61 atcgggatct ttatatttat tgaagatcgt ggctcgcacc gtgctcaaaa agccgcccac
      121 tgaactgaac gtatcattca ctgcgctagc ttcgtatccg ctatggacca tgcaattcgc
      181 gcacttcggg ttcccgctct cagtgccgta ttgatcccaa ttggtctcgc ggatcagctc
      241 ttcgaaagtg tccgcgtatc catcctgcaa aagatagcaa ggcttctgcc acccgaaaat
      301 gttgtaagtc ggcatgcccc agggcgtgca tttgtaatcc cgcttgccca tcaggaattc
      361 gaggaacagt gggctgaggt tgaagcgcca ctcgtcggaa cggttgctca gaatttggcg
      421 aaatagattc cgcgtttttt tcctggagag gaaatgctcc tgatccggag ccttgtcgta
      481 cgtgtagccg ggcgaaagca tcatcccttc gacgcccaat tccatcatat cgtcaaaaaa
      541 ctggcggacg ctttgcgggt cggcgccctc aaaaagagtg gtattcgttg tgacgcggaa
      601 gccgcgcgcc agcgccatcc gaattccctc gacggccttg tcgtacgttc cctcgcggca
      661 aaccgaaaag tcgtgatgct cgcgctggcc atccatgtgc accgaaaacg aaagatactt
      721 actgggcgta aactcgccga ggcgttcgct tagcagcagc gcattcgtgc acaggtagac
      781 atactttttg cgcgagacga gcccattcac gatttcggcg atctgcggat gcatcaaagg
      841 ctcgccaccc ggaatgctca ccaccggcgc gccgcattcg tccactgcgc ggaaacattc
      901 ctctggtgtc aaattcttct tgagaacgtg cgcgggatac tgaatctttc cgcagccagc
      961 gcacgcgaga ttgcaccgca gcagcggctc cagcatcagg acgagcggat actgtttccg
     1021 ccccttcagc ttctggcgga ccacgtagga cgacaccgcc cacatctgcg atacaggtcc
     1081 cggaacagcc atcaataaac ctcgttcatc ctttatcgcg ctctgccaac gttattgatt
     1141 atttgatatc ttaatgatgc cagattccct cggttctgtg aaccagcgaa acttcatagg
     1201 tgtgcgaagc ggatgtacaa caagacgtcg caaggttagc ggccgctgcc cgaatgcggg
     1261 ccaacgattg cggcattagt tttaaaaatc aattagattc tgccttcgat ggctcgcctg
     1321 cgtatgtgga atgcagccgc aatacttcct gcacgcgcgc gtccttgatc accttggcaa
     1381 atgcaggatc cttctctacc tcgagcattt tcttgtaccc atcgtcgcgc gcgagtttca
     1441 ggtagcgcgc cacacgctcg gcatctccca atttggcgta ggattttgca atcgtaaagt
     1501 aaaggtcccc ggaatccgcc gccgttcgct gttgcaaaac cgaaccgctc gatcccctgt
     1561 gctcgaagac catgggatcg agcgcgacag ccttgccgaa cgcttgtatc gcctccggat
     1621 atttcttgat agcgcagtaa gcgtagccga gattgctata ggccgcggcc gtttccggcg
     1681 ccacagtgag ggccttcttg taatatttga tcgctttgcc gtagcgtttc ctcgagtatt
     1741 ccacggtccc gagattgttg atggcctgcg aaaatttctt gtccgcgtgc acggctttct
     1801 tatagaaatg ttcggccagc ctgtcctgcc ccagttcctg gtaggccatc cccgtgcgat
     1861 tgagaaagtc ggcgttgtgc gggtcggttt ctagaagatg ctggtaggcg atggcagcgt
     1921 cgctgaattc ttttcgcgcc atcaagatgg ctgcgcgcat ctccgccgtt tgccgcggag
     1981 tcattggcgg attcgcgttt tccacctgtg ccccgtgtaa atgatccgcc gctggctgga
     2041 cactatcgct ggtattctgc tgagcgcggc aatcccggct gaatccaact gcgaaaaaag
     2101 taactagaat ggcgaagagg gccaatcgtt tcattgcccg tctcctggct gtaccgtggc
     2161 agaggcgacc gctgtattct aatgcggttt cgacgtgtcc ggctgcttct cttccggctg
     2221 tttctctcct ccaaagatac caaatatctt gtcgaagaga ccttttttct tctcgggttg
     2281 cttggtttgc ccctggggcg ccgcctcctg cgccgatttt gccggcggag gaattgcaac
     2341 gtgtccgttt ttagtggcgt tcggcggtgc ctcgtgcgtc tcgggcggtc gaggccccgc
     2401 agcctccgat cccttgccaa acaaatgacc gagccaggat gcaggcccag actgcgccag
     2461 agatcctccg tgcaattggc aatactggtt tggttcagtg cccgatatga aatattcggt
     2521 catgatcttc ggacacgaag gcgtggccag ttgccccgtc tgcggatcga cggattgcgc
     2581 aacgatgcct ggcggaggct caaaattctg agtgtcgcga tatcttggca gcgccacggc
     2641 gcgcttcatg aactctgccc agatgggcgc ggcggcttgg ccgcccgcca accccaaatc
     2701 ccggttgtcg tcgaacccca cccatacgac gcatagcagg tttgacgtgt atcccgcgaa
     2761 ccagccatcg tgcgaggttc ctgtcttccc cgctgccggc gcggtaaatc cgatctgtcg
     2821 caccgggtac ccggtgccgt gattgatcac gtcctccatc aaagtggtcg tcaaaaaagc
     2881 cacgcgcgga tcgagtacgg cccgcgtcga tggagcattc aattccgcag tgctgccgtt
     2941 tgctgcaatc acgctacgga taaaaagagg gtcggcccgc accccattcg ccgcgaacgc
     3001 cgtgtaaccc gccgccactt cgatgggagt catgtcgtac gcgccaagcg ccacggcagg
     3061 cgtggcctgg atatttccgc ccagccccat ctgccgcgcc aagtcaacta cccgttgata
     3121 cccgatcaat tccgccacct tcaccgtggc cacattcagc gaatggatca gcgcatcgcg
     3181 taccgttacg gtcccataga attcttggcc gtaattgttc ggtgtgtagt ccttgccgtc
     3241 gaattcaaat gtggtcggac tatcgtcgat cgtcgtcacc ggcgtaacca ccggctcaag
     3301 gttctcgaca gcatcgtcga aagccgccgc gtaaacaaac ggcttgaaca ccgatcccgg
     3361 ctgccgccgc gacagcgcgc ggttgagctg gctttctccg taattccggc cccctatcag
     3421 cgcccggatc tctcccgtat gcggatccat caccaccacc gccacctgcg ctggcggcac
     3481 ggcttgtccc ttcttcttcc agatcgcgta gcgccgcgcc agcagcttgt cgagattttt
     3541 cacgccgatc tgcaccgctt cgctggccgc gcgctgcaag tcgggatcga gcgtcgtgta
     3601 aatgcggtag ctctggcttt ccagatcgct ctccgaaaat ttctcgagta aatggtcctt
     3661 caccatatcc acgaaatacg gcgcgggact gctttccacg ccgccgctca cgaacttcag
     3721 cttggtcttc ttcgcggctg cttgctcatc cgccgttatg tagcgatcgc cggccatctc
     3781 gccgagcacg cgatcgcgaa cttcgtcggc gcggtccaca tgccgctcgg ccgatgaata
     3841 acggttcggc gcgcggataa tcccggcgag aaacgccgcc tcgccggccg ttaaatcccg
     3901 cacgtccttt ccgaagtacg cctgcgccgc ctcgccgaat ccgcggattg aaaaactgcc
     3961 ccggttcccg agataaatgt cgttcgcgta aagctcgaag atctgctgct tcgaaaaacg
     4021 ctcgtcgagt tcggcggcca tcagcacttc cttcagcttc cgccgccatt cgcgtttcgt
     4081 agtaaagaaa aacgtccgcg ccacctgcat gtcgatcgtg ctggcgccct gggccttgcg
     4141 gttctggctc aggtcgtaaa acgccgcccc gaacacccgc accatgtcca gcgctccgtg
     4201 ctcgaagaat cgcttatctt ccgc
//
LOCUS       DATNDT010000191         4546 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3746874, whole genome shotgun sequence.
ACCESSION   DATNDT010000191 DATNDT010000000
VERSION     DATNDT010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4546
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3746874"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..420)
                     /locus_tag="VN788_06235"
     CDS             complement(<1..420)
                     /locus_tag="VN788_06235"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HXU20164.1"
                     /translation="MVSPVAHRVSAKVHRSLRKAHSKSPAFDVNLFLASAGLGRNVVK
                     FRERETIFAQGDPAKNVMYIQEGGVKLTVVNEVGKQAVVAILGPGDFWGEGCLAGQLI
                     CRATATTVAPTTVLVIEKNEMIRVLHGEHEFSDRFIAY"
     gene            complement(547..1203)
                     /locus_tag="VN788_06240"
     CDS             complement(547..1203)
                     /locus_tag="VN788_06240"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU20165.1"
                     /translation="MKRTKKMASSLSGFSSGASLGGKVSRYRDKQSIYKQGAPAYTLF
                     YIQEGGVRLSTRTKQQPAAVTAILGVGDFFGEICLAGYPLRTSTAVALTATSIRTIKK
                     EKMLQMLRKKGNKTSNSLVAYLLSSVQKYKDHVSELLTSSAEQRLARVLLRLAHLDKG
                     GRPAVEIPILSHQILAEMVGTTRPRVNLFMNRFRTQGFINYDGGLEVHPSLRKVLQNR
                     "
     gene            complement(1405..2274)
                     /locus_tag="VN788_06245"
     CDS             complement(1405..2274)
                     /locus_tag="VN788_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20166.1"
                     /translation="MVLCIALLAGGPDVRPNPAASRQSPTGVLHVPELDDGFHLLYEL
                     KLEEARAQFEAWQKSHPQDPLGSASEAAAYLFEECYRQGILTSDFFLDDKRFLGKVPL
                     KPDPELRAAFFAADKRAQALGQALLKTNPDDTNALFAMTLSVGMQADYASLIDKHQLD
                     SLKMIRAADRYAKKLLAVAPDAADAYLTLGAANYIIGSLPGTKRFFLGFAGIHGNKQL
                     GIQQLQIAADHGHYLRPFAKILLALAALRENKPEVARTQLNDLVAEFPENPLFASELA
                     KLRVAPVASIRSQ"
     gene            complement(2722..3915)
                     /locus_tag="VN788_06250"
     CDS             complement(2722..3915)
                     /locus_tag="VN788_06250"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXU20167.1"
                     /translation="MAQALSQRLPTSEAHVLKLLSQGAPMVEVLHELCNFIDAKSPGV
                     IPTVLLPDSDGHLRLAAGPKVPRIWNEAFDGMKLHSYASFLSMAGHEEGPVPVADMRS
                     DPSFAACWDLALSQGVQAAWSVPILSKERKILGTLILFYPTPQPPGERDLELMELVIH
                     MAAIAIERHRSEEELHAYCSRLHHSQDEERRRIARELHDSTGQKLAVLTMNLSLVRDR
                     IPTQPREIETAFSQCISLATSISDELRTLSYLLHPPLLDDCGLETAIQWYVSGINQRN
                     GLHVEAEIAHELRRLTEEAELAIFRVVQASLTNVHLHSKATQATIKIEQNSDEVIVTV
                     RDNGKGIADGVLDHSSRVKTVGVGIAGMRERMEQLGGRLEIETSCCGTKVNARVPKTH
                     FRAAT"
BASE COUNT         1064 a         1232 c         1211 g         1039 t
ORIGIN      
        1 ataggcgatg aaacgatcgg agaactcgtg ttccccgtgg agcacccgaa tcatctcgtt
       61 tttctcaatg acgagcacgg ttgtgggtgc aaccgttgtc gcagtcgccc tgcaaatgag
      121 ttggcctgca aggcaccctt cgccccaaaa atcgccgggt ccaaggatcg ccacaactgc
      181 ttgcttacct acctcattga caacggtaag cttcacaccg ccttcctgaa tgtacatcac
      241 atttttggca ggatcgccct gcgcgaagat ggtttctctt tctcggaact tgacaacgtt
      301 tctccctaga ccggcggagg cgaggaataa gttcacgtca aatgcaggac ttttggaatg
      361 cgccttccga agagagcgat ggaccttagc ggatacacga tgagcgacgg gagaaaccat
      421 ctcaaccgaa gcgtagcatg aagctttagc cttgtggtgt tcaatattgg acagtcggca
      481 tgctacccgg cacaccggag gaaaatttcc ataaaagcga ggcgcttccg gggttaccgc
      541 taactgctaa cgattctgaa gaactttccg caaagacgga tgaacttcta atcccccatc
      601 gtaatttatg aatccctgcg tcctgaagcg attcatgaac agattgactc gcgggcgcgt
      661 ggtcccgacc atctccgcga gaatttgatg gctcagaatg ggaatctcaa cagccggccg
      721 gccgcctttg tccaagtgcg ccagccgcag aagcacgcga gccagtcgct gctcggcgga
      781 agaagtcagt aattccgaaa catgatcctt gtacttctgg acgctggata gaagatacgc
      841 caccagggaa ttcgacgtct tattaccctt tttgcgaagc atttggagca tcttttcttt
      901 cttgatagta cgtatggaag tggcggtcaa cgccacggcc gtcgacgtac ggaggggata
      961 gcctgcaagg caaatctcgc cgaaaaaatc acccaccccc agaatggcgg taaccgctgc
     1021 tggctgctgt ttcgttcggg tactgagtcg caccccacct tcctggatgt aaaagagcgt
     1081 gtaagcaggc gcaccttgct tatagatact ctgtttgtcc cgatagcgcg agaccttgcc
     1141 tcccaagctg gctcctgaag agaaaccact aagggaggaa gccattttct tcgtgcgttt
     1201 cattcaagct ccatacattc ggctaacata acaggactcc ttcgacgtgg ctgtgctaaa
     1261 gcagacaggg ccggataacc gtctcgatgt atatttgaat cggtcacccg ccttcgcgcc
     1321 aagcgatcga tgatctgcgg cattgttcga gaccaacgcc agatgcgaac cgcccaaaga
     1381 catttgtgct ccagcgcgga tttgctattg agagcgaata gaagcgacgg gagcaaccct
     1441 gagcttcgcc aactcactcg caaagagcgg gttctccgga aactcggcga caaggtcgtt
     1501 aagctgcgtg cgcgctactt ctggcttgtt ttcgcgcagg gccgccagcg ccagcagaat
     1561 cttcgcaaac ggccgcaggt agtggccatg atcggctgcg atctgtagct gctggatgcc
     1621 tagttgcttg ttgccgtgga ttccggcgaa gccgagaaaa aacctcttgg ttccagggag
     1681 acttccgatg atgtagttcg ccgcacccaa cgtaaggtag gcgtcggcgg cgtccggcgc
     1741 gaccgccaac agcttctttg cgtacctatc ggccgcgcgg atcattttca ggctgtcgag
     1801 ctgatgtttg tcgatcaggc tggcataatc ggcctgcatg cccacgctca gggtcatggc
     1861 gaacagcgcg ttcgtatcgt ccggattcgt tttcaatagg gcctgcccca aggcctgcgc
     1921 ccgtttgtct gctgcaaaaa atgccgcacg cagctccgga tcaggtttaa gagggacctt
     1981 gccaagaaaa cgcttgtcat caaggaaaaa atccgaggtc aggataccct gccgatagca
     2041 ctcttcaaaa agatacgccg ccgcctcgga ggcgctcccg agagggtctt gcggatggga
     2101 cttctgccag gcctcaaact gcgcgcgagc ttcctcgagc tttagttcgt agagcagatg
     2161 aaagccatcg tccagttccg gtacgtgcaa aactccagtc ggactctgcc gggaggctgc
     2221 cgggttcggt cgaacatcgg ggccccctgc cagcagcgcg atgcatagaa ccaaaaaatg
     2281 aactccagac atcggcgaaa accgtttcgt gtctctcgca gacatacata tcattttgat
     2341 gccaggcgga gcaggaaggc agcgtcatac gatctcaaaa ctttgccttc cgctttgcac
     2401 agttgtcgga ttaggttcaa cgtggctggt aaactcggcg aaagagtttt ggcgatgggg
     2461 acgatttcca actcgcgatc ataagcagcg gcggcacaaa gggtatgttt agaaatgctg
     2521 ataaggccgc acaacaacat ccggaatagg ccgcagctaa tcgattattc ggttcgacgg
     2581 ggattttgtt tctactccgc gatgcgaaag ccttagcaca gtgaagctcg ctggcaagcc
     2641 cgagcttttt catgagccgg atgcgacacc aattaaaatc tatcatattg ccgagccgaa
     2701 acgggtttgc gttatgatga tttatgtagc tgcgcgaaaa tgtgtttttg ggactctcgc
     2761 gttcaccttt gtgccgcagc agctagtttc aatctcaaga cgaccaccga gctgttccat
     2821 tctttctcgc atgccagcga tcccaacgcc gaccgtctta actcgcgaag aatggtccag
     2881 cactccatcc gctatccctt tgccgttgtc ccttaccgtt acgatgactt catcggaatt
     2941 ctgctcgatc tttatggtcg cctgggtggc cttcgaatgc aggtgaacat tagtgaggct
     3001 agcctgcacg acgcggaaga tcgcaagttc ggcttcttcc gtcagtcggc gcaattcgtg
     3061 agctatctct gcttccacat gcaaaccgtt ccgctggtta atcccgctca cataccactg
     3121 gattgctgtt tccaaaccgc agtcatcaag caggggcggg tgaagcaggt aggaaagggt
     3181 tcggagttca tctgaaatgc tcgtagcaag agatatgcat tgcgagaaag ccgtctcgat
     3241 ttcgcgtggc tgcgtgggaa ttctatctct gacaagcgac agattcatcg tcaagacggc
     3301 aagcttctgg cccgtactgt cgtgcaactc acgagcaatg cggcgacgtt cctcatcttg
     3361 ggaatgatga agccggctgc aatacgcgtg tagctcttct tcactgcgat ggcgttcgat
     3421 agcaattgcc gccatatgga tcaccagttc catcagttcc aggtctcgtt cgcctggagg
     3481 ctgaggcgtt ggataaaata agatcagagt tcctaagatt ttccgctctt tcgacagaat
     3541 cggcacggac cacgccgcct gtacaccttg actaagggcc aggtcccagc aagctgcaaa
     3601 agagggatcg ctcctcatgt cggcaaccgg gacgggccct tcctcatgtc cggccatgct
     3661 caggaaactg gcataggagt gcaatttcat accgtcgaat gcttcattcc agatcctggg
     3721 cactttgggt ccggcggcga ggcggagatg cccatcgcta tccggcaaaa gaactgtcgg
     3781 tatcacccca ggtgacttgg catcaataaa gttgcagagt tcgtgcaaga cttccaccat
     3841 cggagcaccc tgggagagca atttgagtac gtgggcttct gatgtcggta gccgctgaga
     3901 aagagcttga gccatcttgg ttctccttcc tctttacaga ttcggaacag gacacgcttt
     3961 cttggacaaa ctacttccta tggtagagac cacgagaagg ggagaatggt accggtatca
     4021 gtgtaggcag actgttcaat tcgggacaca ttacactgtg catcaaaaat gacgcttttt
     4081 gagacgaaac acggttgtct gtgagaaccc tcaatgagcg cgggctgctg ctatggattt
     4141 cgtgcgtctt ctcaacttct cgccgtcccg cactcaatcc tgagaacggc ctccgagtag
     4201 gacatcacgc tgtcggaatg cgctgcctgc gaacggcata ttaacttata gactaaacca
     4261 ccattcgcga taaatacgac tctttgacgt tgccgatccg aatgtgacgc ttcgcacaag
     4321 gatcgtccgg acatttcgta actgtgcgga atgagactgt atggcttccg tctcgatgtc
     4381 atgatggatc catgctgcat aagaaaggca agaaggaatc cgatgcctcg acgagaaccg
     4441 acgtcagaga tgaaatggca aagccgcgca tggcgaggtc ggcgagaata gcggaattga
     4501 atctcgatac ggcgcgaacc ccggaacaac ccagtgtcgc catgcc
//
LOCUS       DATNDT010000192         3532 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2606125, whole genome shotgun sequence.
ACCESSION   DATNDT010000192 DATNDT010000000
VERSION     DATNDT010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3532
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2606125"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VN788_06255"
     CDS             complement(<1..331)
                     /locus_tag="VN788_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019239716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXU20168.1"
                     /translation="MAKEKFERVKPHVNVGTIGHIDHGKTTLTAAITKVLSKNNPKVS
                     FRAFDSIDNAPEERERGITIAVSHVEYETANRHYAHIDCPGHADYVKNMIPGAAQMDG
                     AILVVEAT"
     gene            complement(438..512)
                     /locus_tag="VN788_06260"
     tRNA            complement(438..512)
                     /locus_tag="VN788_06260"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Gly,seq:tcc)
     gene            complement(536..611)
                     /locus_tag="VN788_06265"
     tRNA            complement(536..611)
                     /locus_tag="VN788_06265"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(576..578),aa:Thr,seq:ggt)
     gene            complement(653..741)
                     /locus_tag="VN788_06270"
     tRNA            complement(653..741)
                     /locus_tag="VN788_06270"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(705..707),aa:Tyr,seq:gta)
     gene            complement(958..1030)
                     /locus_tag="VN788_06275"
     tRNA            complement(958..1030)
                     /locus_tag="VN788_06275"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(995..997),aa:Thr,seq:tgt)
     gene            1524..2540
                     /locus_tag="VN788_06280"
     CDS             1524..2540
                     /locus_tag="VN788_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20169.1"
                     /translation="MTSSQKKRLLIALLAIIAVIAGAVYLHLARRAASPATAALAPNI
                     LTLLPPHAPAIAYIDVTALRRLQNSPLAAILGLTQSGPQPDRDYQDFVRDTGFDYTRD
                     LDKAAIAFWPAISSSGYISENQVVAIADGHFDQPKIKAYALRTGRVVTRNARSIYEVP
                     GNPRVSFEFLSSTRIALASGPTADNLLPPSTSSTPDPAMQARINRVAGAPIFAIARTD
                     NLPPQFYESLRSSPQFQSLAHSVQGLALAGRPDGNLIHLTLEAECNSMKNAIEWVTLL
                     NSLRIFGPIALADPKTRSQMTPSQISLLETLINKTTITHQDRLVRLSLDLTPAMLGAA
                     NSKP"
     gene            2613..3224
                     /gene="ruvC"
                     /locus_tag="VN788_06285"
     CDS             2613..3224
                     /gene="ruvC"
                     /locus_tag="VN788_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXU20170.1"
                     /translation="MPSDSMPTIKKARKLRILGVDPAVTGATGYGVIEIDGSAARPMR
                     FGALRLPSRATFAVRLREIHCLISGLVEQFSPDAVAVESVFTALNMRTALKLAEMRGV
                     VLLAAAQAQIPAHSYSPREVKASVAGFGGASKQQIQQMVRSLLDLPDCPQPADASDAL
                     AVALCHAQATRARDRMATASLRWTVSVAASGKPRASRAPILRA"
     gene            3294..>3532
                     /locus_tag="VN788_06290"
     CDS             3294..>3532
                     /locus_tag="VN788_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20171.1"
                     /translation="MGLGTLAARHETNFLALARRAAVLGAFLLLPLAAVAHAVAPAPS
                     GATITFRKIFKSSYPEFVEIKVTESGTGTYDIRQL"
BASE COUNT          726 a         1190 c          830 g          786 t
ORIGIN      
        1 ccgtcgcctc caccaccaga atcgctccat ccatctgcgc cgcgcccggg atcatgttct
       61 tcacataatc cgcgtgaccc gggcagtcga tgtgcgcgta atgacgattc gccgtctcat
      121 actccacgtg cgacaccgcg atggtgattc cgcgctcccg ctcttccgga gcgttgtcaa
      181 tcgaatcgaa cgctcggaac gataccttcg ggttgttctt cgatagcacc ttggtgatcg
      241 ccgccgtcaa cgtcgtcttc ccgtggtcga tgtgcccaat cgtccccacg tttacgtgcg
      301 gcttaaccct ctcaaatttc tccttcgcca tctctcgtca ccctcgcatt aagacttcgc
      361 ggcagcagcc gcgccaaatc tcttcacttc cccgaggtgt cgtccaactc aagctcccaa
      421 caaataaccc tatcctatgg agcgggggac ggggctcgaa cccgcgacca tctgcttgga
      481 aggcagagac tctaccactg agttaccccc gctcatgcct tgctcgcctc caacatggag
      541 cctgggaccg ggattgaacc ggtgacctcg tccttaccaa ggacgcgctc taccaactga
      601 gctacccagg cccgaaccgg ttcccttatt ctttccgaac ctccgcgaaa gctggtggac
      661 gggggaggat tcgaacctcc gtagcccgca aggagcggca gatttacagt ctgctggttt
      721 tagccactca cccacccgtc ccgggatgca ctggaatgtc aattcagaac tcggtgaaat
      781 gcatccggga cacagctatc gtatcccgct aacccaacca gatctcaacc ggactgctgt
      841 acgggggtgc tccgcaaacg cagaaagggc cgtgtccaga gacaccagcc ctgttcgctt
      901 ttctcgcgag gaatccgtcc gtatcacaaa ccgaaaaatc tcggtcccct caaaatcagc
      961 tggcggaggg agttgaaccc ccgaccctct gattacaaat cagatgctct accaactgag
     1021 ctacgccagc cccgcgcaaa ccgagtaaaa atatcacgca ggaactaaaa ttgcaagcaa
     1081 gttccccgcc ccagcaaaac cgttgccact gttcaactta atatcggaac gctaagtata
     1141 ccatgccgta tcgctcaccg gctacgctaa ctttcctgcc caggcttttc gcgctccctc
     1201 gcttccgcct aagaaacacc gaaagtccac actcaccgtg gacgattttt ctttacacca
     1261 acaactactt gcgcgattcc gaccctgcat gtaaagattt tgtctgtatc ctcggacttt
     1321 gcccacggct gcatcacgcc aaactcacaa tatagaaagc tccgaagagc cgctccgctc
     1381 tcccaagcgc tgcgaattca aattgttttg tatatctatc agacgcattt tcagcatctc
     1441 aggttgcacg ctttttttct tctgccatcg cgcggccaga catacactag atcgccttaa
     1501 gcgaatacaa ctctcattct ttcgtgacct cttctcaaaa aaaacggctc ctgattgcac
     1561 tgctcgccat aattgctgtg atcgccggcg cggtctatct gcatcttgcc cgacgcgcag
     1621 catcgccggc gaccgctgcg ctggccccaa acatcctcac tcttcttcca ccgcatgcac
     1681 cggccatcgc ttatatcgac gtcacggccc tgcgccgatt gcaaaattct ccgctcgccg
     1741 caatcttggg tctcacccag tccggaccgc agcctgatcg cgactatcag gatttcgtcc
     1801 gcgataccgg cttcgattac acacgcgatc tcgacaaagc agccatcgca ttttggcctg
     1861 cgatctcttc atccggctac atcagcgaaa accaggtcgt cgcgatcgcc gatggacatt
     1921 tcgatcaacc taaaatcaaa gcgtacgcgc tccgcaccgg acgcgttgtc acgcgcaacg
     1981 cccgttcgat ctacgaagtg ccaggcaatc cgcgagtttc attcgagttt ctttcatcca
     2041 cgcgcatcgc tctcgccagc gggcccaccg cggataattt gctgcctcca tcgacttcct
     2101 cgactcccga tccagccatg caggcgcgca tcaatcgtgt tgcaggcgcg ccgattttcg
     2161 ccatcgcgcg cacggacaac ctcccgcctc aattttacga atctctgcgc agttccccgc
     2221 agtttcaaag cctggcccat agcgttcagg gtctcgctct cgcagggcgg cccgacggga
     2281 atttgatcca tctcactctc gaagcagaat gcaattcaat gaaaaacgcg attgaatggg
     2341 tgacgcttct caatagcctc cgcattttcg gccccatcgc tcttgccgac ccgaaaaccc
     2401 gcagccagat gacgccttcg cagatctccc tgctcgaaac tttaatcaac aaaacgacaa
     2461 tcactcacca ggatcgcctg gttcgcctct cactcgacct aaccccggcc atgctggggg
     2521 ccgccaattc aaagccttag ccggctgcgg gaacaacgca tcaaaaaact tcttcttcca
     2581 gtttccccac gcgatatagt tgtgccggat ttatgccttc tgattcaatg cctacaatta
     2641 aaaaggcccg caagctgcgc atcctcggcg tggaccctgc cgtgacaggt gccaccggat
     2701 atggcgtgat tgaaatcgat ggctccgccg cccggcccat gcggtttggc gcattgcggc
     2761 tgccatcccg cgccacattt gcagtccggc tgcgcgaaat ccactgcctg atctctggac
     2821 ttgtcgagca attttcgccg gacgccgtcg ctgtcgaatc ggtcttcacc gcgctcaata
     2881 tgcgtaccgc cctgaagctg gcggaaatgc gcggcgtggt acttcttgca gcggctcagg
     2941 cgcagattcc agcgcacagc tactcgcctc gcgaagtgaa agccagcgtg gcgggcttcg
     3001 gcggagcttc taagcagcag atccaacaga tggtgcgatc gcttctcgac cttcccgatt
     3061 gtcctcagcc cgccgacgct tctgacgcgc tcgctgtcgc tctctgtcat gcgcaggcca
     3121 ctcgggcgcg cgatcgcatg gccacggcct cgttgcgctg gaccgtttcc gttgctgctt
     3181 ccggcaaacc tcgcgcatcc cgcgctccca tccttcgcgc ctgacctgtt gcattttcgg
     3241 tgtggaggga tttagagtca tcaagttcac tttcaattct cgaggtgggt gtagtgggtc
     3301 ttgggacttt ggcagcacgg cacgaaacta acttcctcgc gcttgctcga agggcggcgg
     3361 ttcttggagc tttcttactt ctgccgcttg ccgccgtagc gcacgccgtg gcgcccgcgc
     3421 cctccggcgc caccatcacc tttcgcaaaa tcttcaaatc cagctatccg gagttcgtcg
     3481 aaattaaggt aaccgaatcc ggcaccggca cgtacgacat ccgccagctc ag
//
LOCUS       DATNDT010000193         5428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2090695, whole genome shotgun sequence.
ACCESSION   DATNDT010000193 DATNDT010000000
VERSION     DATNDT010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2090695"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..529
                     /locus_tag="VN788_06295"
     CDS             56..529
                     /locus_tag="VN788_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20172.1"
                     /translation="MFEAQTKEKSGGSGPLIVICIVVVLAVVGTLAYLNRGPAKSSEA
                     SASSATGAKPAGLKGNPVKDLHIVSAKMEKDYTGNLAVWSVDIRNQSQVYTYSNIGYQ
                     TNYFGGDNSVLSQNKGNISLSLGPGDEQSTQFRDVLYPDNTAWYRFTVVGADTSK"
     gene            complement(599..1456)
                     /locus_tag="VN788_06300"
     CDS             complement(599..1456)
                     /locus_tag="VN788_06300"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXU20173.1"
                     /translation="MRPTLPAMNSDTQRPEPEIADPHEIRQSLVHLDRSTAWFRWNTI
                     GALTLLFAALVILSWPNSFGHWATVIVQGLLGLMLTANSYMLYQHRQFKHFRRRLGEQ
                     MQTAITQRERADKFYGLAILDPLTGLYNRRYGEECLRKEVMRAERQNYDLAVIVLDLD
                     RFKEINDQFGHAAGDLVLKEFANRLRRSIRACDVPVRIGGDEFLVVLPECPRENVHII
                     LSRLEPFEVILNRRKVLVSYARGRAQYQVADTLETLLQRADEVLYAEKAGRSVATAQS
                     DDDQSVLSI"
     gene            1753..3192
                     /gene="cysS"
                     /locus_tag="VN788_06305"
     CDS             1753..3192
                     /gene="cysS"
                     /locus_tag="VN788_06305"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HXU20174.1"
                     /translation="MNEIRLHNTFTNALGVLDPVHPGEVRIYTCGPTVYASAHIGNFR
                     TFMFQDILRRFLRARGYSVLQVMNVTDVDDRIIQSAAAAGVGIREYTDKYIQAFLEDI
                     DALHIEMPEELVRATDHIEDMVALIEKLQQKGLTYTTDGSIYFRISKFPAYGKLSKID
                     VSGMQAGARVDVDQYEKENARDFALWKAPKPGEHFWETRIGPGRPGWHIECSAMAMKY
                     LGDTLDIHTGGVDLAFPHHENEIAQSEAATGKTFVRTWLHAEHLIIEGQKMSKSLGNF
                     YTLRDLFAKGHKPSTIRFLLLSVPYRRQLNFTEEGLLQARNSTERLRNFVTRLKAEQF
                     PAGSNPEIEKQIAAAENDFDAGLSDDINTAMALAAVFDLVREANTAMDRGAFLQQNAP
                     QAVAAMEKFDSVLALITDDDEEKLRKLGIGLAKPRMSSEQVEGLIEERTAAKKRRDFE
                     RSDEIRQNLADSGILIEDMKDGSVRWKYK"
     gene            3354..4706
                     /locus_tag="VN788_06310"
     CDS             3354..4706
                     /locus_tag="VN788_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HXU20175.1"
                     /translation="MQVAAKKRKLPHIITYLPGPKAREIVERDRKVVSPSYTRDYPLV
                     AKSGSGAIICDADGNEFLDFAAGIAVVATGHCHPEVVSAIQKQAAQLIHMSCTDFYYE
                     GTVQLAEKLASIAPGTGAKRLYFGNSGTEAIETAMKLARYHTKRDKFIAFYGCFHGRT
                     MGSLSLTASKAIQRKNFGGLVAGVFHAPYPNTYRGAYGVRPEHAASDALAQIENELFK
                     KLVDPEEVAAIFVEPIQGEGGYVPAPAEFLQGLERLCRKHGILLVADEVQSGMGRTGK
                     WWAVDHSGVEPDIICVAKGIASGMPLCAVIAKAEVMDWKPGAHASTFGGNPVAIAASL
                     ATIGVLEREAMANAARMGEFVFRKTADWRERHKSVGDIRGKGLMIGIEFVRDQKTKER
                     APNLRNRVIQEAFQRGLLVLGAGENSLRLAPPLIIHEEQAEFAIDTLDACIGAAERAG
                     "
     gene            4723..5208
                     /locus_tag="VN788_06315"
     CDS             4723..5208
                     /locus_tag="VN788_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXU20176.1"
                     /translation="MIARLIFAMVSFAARKGLSESRDTDSREVTWKQRARRTGIRGET
                     YAYWYLRRQGYTMVARNYVSTGMKGEIDLIGYDGAVLAFVEVKTRKAADGDRPTPEEA
                     VTREKQGHLTRMARQFLRARHIDGGSWRFDVLAIEENAGRRPEVRLHKGAFVPHAGWQ
                     A"
     gene            5310..>5428
                     /locus_tag="VN788_06320"
     CDS             5310..>5428
                     /locus_tag="VN788_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015724659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HXU20177.1"
                     /translation="MPELRKDPITGRWVIISTDRAKRPTDFIRESVQIKGVGM"
BASE COUNT         1277 a         1418 c         1665 g         1068 t
ORIGIN      
        1 aagcgcgtgc cactcgcacc gcgataggaa atgggcaagg taaggaggga ccaaaatgtt
       61 cgaagctcaa acgaaggaaa aatcgggcgg atcggggccg ctgatcgtca tctgtatcgt
      121 tgtggtattg gcggtggtgg gtacgctggc ctatttgaat agggggccgg cgaaaagctc
      181 ggaagcatct gcatcttccg cgaccggcgc caagccagcg gggctgaagg ggaatcctgt
      241 aaaggatctg cacatcgtga gcgccaaaat ggagaaggat tacaccggga atttggcagt
      301 gtggtcggtg gatatcagga atcagtccca ggtgtacacg tacagcaaca ttggctatca
      361 aacgaactat tttggcggcg acaacagcgt tctgtcgcaa aacaaaggga acatctcgct
      421 gagccttggc ccgggtgatg agcagagtac tcaatttcgc gacgtgctgt atcccgataa
      481 cacagcatgg taccgattca ctgtggttgg ggccgatact tcgaaatagg ggaaggctag
      541 ccgggtgcgg tgaacatttt gcagctttgt ccggagtgag aagacgggac ggaaattgtt
      601 agattgacag aaccgattga tcgtcatctg attgagccgt agccaccgag cgaccggcct
      661 tttcagcgta cagaacttcg tcagctcttt ggagaagggt ctcaagtgtg tcggccacct
      721 ggtactgcgc gcgcccgcgg gcataggaaa ccaggacttt ccgacgattc aggatgactt
      781 cgaagggctc aagacgcgaa agaattatgt gcacgttctc tctggggcat tcgggcagaa
      841 cgacgaggaa ctcatcgcca ccaatgcgaa ccggcacatc gcaggcacga atggaacgcc
      901 gcagacggtt ggcaaactcc ttcagcacga ggtcgccggc ggcgtgccca aactggtcat
      961 tgatttcctt gaatcggtcg agatccaaaa caatgactgc caggtcataa ttctgccgct
     1021 ccgctcgcat gacctccttc cgcaaacatt cctccccgta ccgccggttg tacaggccag
     1081 tgaggggatc gaggatcgcc aagccgtaaa atttgtctgc tcgctcgcgc tgggtgatcg
     1141 cggtttgcat ctgttcgccg agtcgccggc gaaagtgctt gaactggcgg tgctggtaaa
     1201 gcatgtagct attagcggtc aacatcaggc caagcaatcc ttgtacaatc acagttgccc
     1261 agtgcccgaa cgagttcggc caagaaagaa ttactagggc cgcgaagagc aaggtcaatg
     1321 ctccgatggt gttccagcgg aaccaggcag tgctgcggtc caggtgtacc aagctctggc
     1381 ggatctcgtg aggatcggcg atttccggct ccggtcgctg cgtgtcgctg ttcatcgctg
     1441 gtagagtagg cctcatcccc gccggatcaa atagtacgca aagcctagtt gtcactgatc
     1501 tcttgtacca ttccaagctt ctcagaactg cccgtaatct tcccggcaaa gctcgcgcaa
     1561 cgcgttgaga tcaccctgaa attagaggat agcatcggca cagttcccgg cacggtaggc
     1621 agcgaaaagg tcaacagtta gccaccctac agagcgccga gacaggcggg cgcaaacccg
     1681 acctccatct agcagctcgt ttgcggctga gaagggcgtc ggctacaata cccgttttgc
     1741 ataaaccata aaatgaacga gatacggctc cacaacacgt ttacgaacgc tctcggggtg
     1801 ctcgatccgg tgcacccggg cgaggtccgg atttacacct gcggcccgac tgtctatgcg
     1861 tcggcgcaca tcggcaattt tcgaactttc atgtttcagg atattttgcg gcgtttcctg
     1921 cgtgcgcgcg gctatagcgt gctgcaagtg atgaacgtga ccgacgtgga cgaccggatt
     1981 atccagtcgg cggcggcggc cggggtggga attcgcgagt acaccgacaa atacattcaa
     2041 gcttttcttg aagacatcga cgctctacac atcgagatgc cggaagaact cgttcgcgcc
     2101 acggatcaca tcgaggatat ggtcgcgctg atcgagaagc tgcaacagaa gggcctgact
     2161 tacacgaccg acggatcaat ttacttccgt atttcgaagt ttccggcgta cggcaagctt
     2221 tctaaaatcg acgtgagcgg gatgcaggcc ggagcgcgcg tggacgtgga tcaatacgaa
     2281 aaggaaaatg cgcgcgattt tgcgctatgg aaggcgccca agcctgggga gcatttctgg
     2341 gaaaccagga tcggtccggg acgaccgggc tggcatatcg agtgctcggc gatggcaatg
     2401 aagtacctcg gagatacgct cgacatccac accggaggcg tggaccttgc gttcccgcat
     2461 cacgagaatg aaatcgcgca aagcgaagcg gctaccggga aaacgtttgt gcgcacttgg
     2521 cttcacgccg aacacctgat aatcgaaggc cagaagatgt cgaagtcgct tggcaatttc
     2581 tatacgctgc gcgatctttt cgcgaagggg cacaagcctt cgacgatccg cttcctgctg
     2641 ctttcggtgc cgtaccgacg ccaactgaat ttcacggaag aagggttgtt gcaagcgcgg
     2701 aactcgacag agcggctacg caattttgtc acacgactga aagcagagca atttccggca
     2761 gggtccaatc cagagatcga aaagcaaatt gctgcagcgg aaaacgattt cgacgccggc
     2821 ctatcagacg atatcaacac ggcgatggca ctggcggcgg tgttcgatct ggtgcgcgag
     2881 gccaacacgg cgatggaccg cggtgcgttc cttcagcaga acgctcccca ggcggtagcg
     2941 gccatggaaa aattcgacag cgtgctggca ctaataacgg acgacgacga ggagaaattg
     3001 cgtaaactgg gcatcggact cgcgaagcca cgcatgagtt cagagcaagt cgaaggcctc
     3061 attgaagaac gcaccgcggc aaagaagcgg cgcgacttcg agcggtccga cgaaatccgg
     3121 caaaacctcg ctgattctgg tattctgata gaagacatga aggacggcag cgtccgctgg
     3181 aagtacaaat gagaatttcc ccgagacacc tgaatccctt tgatggccag ccttcccacg
     3241 cgcgcccgaa tcttttcggc gtagattaat tcacgctttt cccgatgatg tatccggctt
     3301 gttgcagaga gccagtccgc atgccggagg aaattaagct ccgggaggaa gacatgcaag
     3361 tcgctgcaaa aaagagaaaa ctgcctcaca ttattaccta tttgccgggc cccaaggcac
     3421 gggagatcgt ggaacgcgat cgcaaagtgg tttcgccttc ctacacgcgc gactatccgc
     3481 tggtagccaa gagcggaagc ggcgcgatca tttgcgatgc tgacgggaac gaatttctcg
     3541 atttcgcggc agggatagct gtggtggcta ccgggcattg ccatccagaa gtggtgagcg
     3601 ccatacagaa acaggcagct cagctgattc acatgtcctg cacggatttc tactacgaag
     3661 gcacggttca acttgcagag aagcttgcgt cgattgcgcc aggcacgggc gcgaagcggc
     3721 tctattttgg aaattcgggc acagaagcca tcgaaacggc aatgaaactc gcgcgttatc
     3781 acactaagcg tgacaagttc atcgcgtttt acggctgctt ccacggacgg accatggggt
     3841 cgctatcgct gacggccagc aaggccatcc agcggaagaa cttcggcggg ctcgtggcgg
     3901 gcgtatttca tgcgccgtat ccgaatacgt accggggggc ttacggagtt cgcccggaac
     3961 acgccgcgag cgatgcattg gcgcagatcg aaaacgaact tttcaagaag ctggtggatc
     4021 ctgaagaagt tgccgcaatt ttcgttgagc ccattcaggg agagggcgga tacgttccgg
     4081 ccccggcgga atttttgcag ggcctggagc gtttgtgccg taagcatggc atcttgctcg
     4141 tcgcggacga ggtgcaatcg gggatggggc gcaccggtaa atggtgggcc gtggaccact
     4201 ccggggtcga gccggatatc atctgcgtgg caaagggaat cgcatccggc atgccgctgt
     4261 gcgcggtgat cgcaaaagca gaagtgatgg attggaagcc cggggcgcac gcgtccacgt
     4321 tcggaggcaa tcccgtcgct atcgccgcgt cgctggcaac tatcggagtg ctggagcggg
     4381 aagccatggc gaatgctgcg cgaatgggcg aattcgtttt tcgcaagacc gccgattggc
     4441 gcgagcggca caagagcgtg ggtgatattc gcggcaaggg gctgatgatc ggcatcgagt
     4501 tcgtgcgcga ccagaaaacg aaagaaagag cgcccaacct gcgcaaccgg gtaatccagg
     4561 aagcgtttca gagaggattg ctcgttcttg gagcgggaga aaattcgcta cgactcgcac
     4621 ctccgctgat tatccacgag gagcaagctg agtttgcaat cgacacgctc gatgcctgca
     4681 tcggcgcagc ggaacgagcg ggctagtttc aaggattttg ccatgattgc gcgactgatt
     4741 tttgcgatgg tgagtttcgc cgctcgcaaa gggctgagcg aatcgcgcga taccgattcc
     4801 cgggaagtca catggaagca gcgagcgcgt cgcaccggaa ttcggggcga gacgtacgcg
     4861 tattggtacc tgcggcgcca gggatacacg atggtggctc ggaattatgt ctcgacggga
     4921 atgaaaggcg agatcgatct gatcggctac gatggagcgg tgctcgcttt cgtggaagta
     4981 aaaacgcgca aggccgccga cggggaccgg cccacacccg aggaagctgt cacccgagaa
     5041 aagcagggac atctcacgcg gatggcgcgg caatttcttc gtgcgagaca catcgacggt
     5101 gggtcttggc gttttgacgt gctggcgatt gaagaaaacg cgggccggcg acccgaagta
     5161 cggctgcata agggagcgtt tgttccacat gcgggttggc aggcgtagat gtatagcggt
     5221 gcgagggctg tggcagaatg gccccgcgat tgctccctgt ttggcaacgt agccggtctg
     5281 cgctacaaac aacgaaataa ggaggaggtt tgcctgagct tcgcaaggac cccatcaccg
     5341 gccgatgggt aatcatttca acggaccgag ccaagcgtcc cacggatttc attcgggaga
     5401 gcgtgcaaat caagggcgtg ggaatgtg
//
LOCUS       DATNDT010000194        11382 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2590575, whole genome shotgun sequence.
ACCESSION   DATNDT010000194 DATNDT010000000
VERSION     DATNDT010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11382
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2590575"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..916)
                     /gene="ccsA"
                     /locus_tag="VN788_06325"
     CDS             complement(<1..916)
                     /gene="ccsA"
                     /locus_tag="VN788_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HXU20178.1"
                     /translation="MDIFGSFALLLALLAAAYAFVAGITGILTRRTLLSKSARNAGMA
                     VCGLVTLAVAALEYFFFTDNFSLAYVAEHSNRALPLFYKFAALWSGQEGSLLFWSWLL
                     SIYAFCALFLNRKKHPELMPYVGVALAGVQIFFLILNVFVASPFHILAGADASGAMHM
                     ARVSEGSGLNPLLQYPEMVIHPPMLYLGYTGFTVPFAFAMAALLGRYPGEKWIHITRR
                     WTMVAWGLLTVGILLGAHWAYAVLGWGGYWAWDPVENASLLPWITGTAFLHSVMMQEK
                     RGMMKVWNVWLVFVTFMLCILGTMLTRSG"
     gene            complement(937..1413)
                     /locus_tag="VN788_06330"
     CDS             complement(937..1413)
                     /locus_tag="VN788_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HXU20179.1"
                     /translation="MKARGKFLTGSGIIVATLLVLAYVGFTQSKTYYHTISELPMLSA
                     PALHQRMRVSGNVRAGSISHADGHINFVLEEQGKDLPVSYVGRDPLPDTFKDGAQALV
                     EGRLMPQGGFVAEGVQAKCASKYEATPPGMAGAKHSAPPVDQSTTEPSVPTSGRKS"
     gene            complement(1596..2525)
                     /locus_tag="VN788_06335"
     CDS             complement(1596..2525)
                     /locus_tag="VN788_06335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXU20180.1"
                     /translation="MSGFKVLVVDDSPVYRKLVEHALSQDSSAVLFATSGHQALEIFE
                     LEHPEIIITDWLMPDLTGIELCQRIREGTYASYIYVIILTSNAEKENVVMGLSAGADD
                     YLTKPFHAAELLARVRVGQRLIGLHRQIKTKSRLLEELALTDALTGLPNRRAIETWAT
                     RQLSGAARHGFPFWVVMIDLDHFKLVNDAHGHDAGDMVLRKFAEILKSNTRLSDISGR
                     IGGEEFLQVITHADEASVALVLERIRSQFGAEKFLVNGAEFVVTASFGVAGFLGKNAP
                     DFSELVTRADAALYRAKHRGRNYVEVEPVAVPL"
     gene            complement(2546..2926)
                     /locus_tag="VN788_06340"
     CDS             complement(2546..2926)
                     /locus_tag="VN788_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20181.1"
                     /translation="MAEQEVPLMADVFRPYERLVEITILGKTFLVPERNSLLRAFQFI
                     SPETIPYGRFCWNQECQYCRVDCKLPDDDAARPILSCKFIVSEGMEISNLAPELIGCL
                     RAKLGQTQAASPEVADGPSDTRKI"
     gene            complement(3057..3500)
                     /locus_tag="VN788_06345"
     CDS             complement(3057..3500)
                     /locus_tag="VN788_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20182.1"
                     /translation="MIFAISGPAAGWSMRPIDKPQGRLGALWTIYGLICLAEAVWVAL
                     NVATLTIMWGTIVSRVANPFSWMSVFRFFLVGIIALAIVTGIFSLLAGFALMKRGSSS
                     RTLALTSSFLGILNGPLGIALGVYTLVLLVPRASWRTHERLPTAA"
     gene            complement(3566..4654)
                     /locus_tag="VN788_06350"
     CDS             complement(3566..4654)
                     /locus_tag="VN788_06350"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HXU20183.1"
                     /translation="MTDSLEKRKLARKRLRRMRALAKRRKLQAERGAAQSTRSSADNA
                     SVKDVNAQRQLEIDALERDISELSRLTDPTDTTAEEIGRIQREVEELKREFYSHLDSW
                     RRLQLARHPQRPYTDDFIRLLFEDFSEVHGDRYFADDPALITGMARFQGRSVLVVGNQ
                     KGRDTKQRLARNFGQAKPEGYRKALRAMHLAAKFGRPIFVFVDTQGAYPGIDAEERGQ
                     AEAIAYNIREIARLPVPILVTITGEGGSGGALAIAIGDRIFMLENSVYSVISPEGCAS
                     IMWRDPTKSEMAADALKITARDLLDLRLIDEIVPEPEGGAHMDHPACAKLVGDVLARA
                     LNQLLLVPPEKLLQQRYEKFRSIGQYFA"
     gene            complement(4651..8127)
                     /gene="dnaE"
                     /locus_tag="VN788_06355"
     CDS             complement(4651..8127)
                     /gene="dnaE"
                     /locus_tag="VN788_06355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HXU20184.1"
                     /translation="MASSASSNFVHLHLHTDYSLLDGACEIGELTAEAARRGMPAVAV
                     TDHGNLFAAANFYHQATTHGVKPIIGCEVYVAPDNHKNRGAEAERSNHLILLCENDEG
                     YRNLIKLVSTGFLDGFYYKPRIDHDLLARHSKGLIALSACLRGEVADALLSEKYEQAR
                     TNAYRLRDIFGKGNFFLEVQDQGLDVETRVNRDLVALSRETGIPLVATNDCHYLTQSD
                     SHAQEVLMCIQTGKTMSDAQRMKFATDQFYFKTAEEMAQVFRELPDAVSRTVAIADRC
                     NVKIQRVSNPFPEFKVPEGHTTDSYFERVVREGFAARAQQLERLAKENRLRHSLSDYE
                     SRLFAEIQMIQKMRFAGYFLIVWDFIRYARSQGIPVGPGRGSAAGSLVSFALQITDVD
                     PLQYDLLFERFLNPERISMPDIDIDFCMRRRGEVIEYVRQTYGAGSVAQIITFGTMAA
                     KAALKDVGRAIDMPYGEVDKIAKMIPATLNIELEDALKQTPQLDSLRKSDERVREMVE
                     VALRLEGLARHASTHAAGVVISPRPLTEIVPLYKTNKDEVTTQYDMNALERLGLLKMD
                     FLGLTTLTVLDDAARLIQQNRGVSVDLATLPLDDPAVYALFSRGDTTGIFQFESHGMR
                     DILRRYQPSRIEDLTALNALYRPGPIQGGMIDDFIARKHGKKKVTYDLPELEEILSET
                     WGVILYQEQVMQIANRLAGFSLGDADLLRRAMGKKKHEEMVAQREKFLTGCHARKVPQ
                     KKAEKIFDLMAEFAGYGFNKSHSCAYALLAYQTAWLKAHYPVEFVAAMLTSETGNTEK
                     VVKYINEARSMGITVLPPNVNSSDMNFTPVGGDIRFGLAAIKNVGENTVKGILAARKS
                     LGQFTSFFQFCESVDPRLINKRVLESLARAGALDGLGAHRAQMIAGIDRAVERAQKIQ
                     RTRESGQHGLFGSGTAVTETPRPEILPDAAEWAEHELLAAEYSTLGFYISGHPLDKYT
                     GRLQEMKAAELASLEGRRNGEEVTVAGIIVQSRAMRSRRGARWAILTLQDRTGVCEAL
                     AFPEAFLKLEAILKGATPLLMKGRIAVEDVGTRLIISDARLLDQVRQQPSAPNLLRVR
                     VNRSAIDAGIIDRLQELFASRPGRCRIAFELVHDDGTEATIDAGSAVRADSEFLNRVR
                     EICGADSVAVMQ"
     gene            8379..9017
                     /locus_tag="VN788_06360"
     CDS             8379..9017
                     /locus_tag="VN788_06360"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXU20185.1"
                     /translation="MVACAVSTANAQWSSTEPHRNFEITPFGGSRFGGVINTSSGGSN
                     VIDEFTIKSTWDYGAIADIDLLPDSLPGLQGEFMWSQEPTTLGARDSLTGIISPAGKL
                     ELNNYQWGIDYNLGSRFSKLHPFVVGGMGFTHFSSGGVLPFENKLSFNLGGGVKYFVV
                     RHAGVRFDVRWVPSRTTSHVETFFDPFFGFFQANVNNYAEQFQANLGLIFRF"
     gene            complement(9044..9289)
                     /locus_tag="VN788_06365"
     CDS             complement(9044..9289)
                     /locus_tag="VN788_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20186.1"
                     /translation="MNLSTIRIVPTGKVQVGQVWKKDGTDDSFLITKIYSEALATYAV
                     LRKAGAEGEPPIRIKVAKNGPAANLPGYTFAQQSDDF"
     gene            9402..10394
                     /gene="hemH"
                     /locus_tag="VN788_06370"
     CDS             9402..10394
                     /gene="hemH"
                     /locus_tag="VN788_06370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HXU20187.1"
                     /translation="MPAEKKIAVVCFQLGGPDRLSAVEPFLYNLFCDPDIIDFPGSFL
                     ARKPLAKLISTTRSKTVRQHYAEIGGGSPIRRLTEQQARALDMALRPHISAHTFVAMR
                     YWHPNTEEAIAALQRVSFDELVLLPLYPHYSLATAGSSLKEWNRLYAPHVPVHVVDHF
                     FDHPDYIAGVVERINGILQQLPYPDEVPLVFSAHGLPMALVEKGDPYPRQIEATVHLV
                     RELGAWANPYVLCFQSKVGPQKWLQPSLTGTIAKMAGEGIKRMLVVPISFLTEHIETL
                     YEINIEAREQAESLGVSDFHMMPALNDSPLLIRALADLTLRAVGRRAGVASLAL"
     gene            10558..>11382
                     /locus_tag="VN788_06375"
     CDS             10558..>11382
                     /locus_tag="VN788_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HXU20188.1"
                     /translation="MEFSLTDEQQHLRRTVREFAEAEIAPHLMEWDEASQFPSEIIPK
                     LAEMGFLGVIFPEKYGGAGMGYLEYAIIIEELSRVDGSVGIIVAAHNSLCTNHIYKFG
                     TEEQKQKYVSPLAQGKKLGCWSLTEPEAGSDAGGTRTVADKKNGGWVLNGAKTFTTNG
                     HYADVCVAMAVTDASKKHHGISAFIIEKGTSGFRPGKKENKLGLRASDTSEVVFTDCR
                     VPPENLLGPEGEGFVNSLQILDGGRISIASLGLGMAQGAYECSVRYAKERKQFGKPI"
BASE COUNT         2437 a         3328 c         3131 g         2486 t
ORIGIN      
        1 cgccggagcg cgttagcatc gtgccgagaa tgcacagcat gaatgtgacg aataccagcc
       61 acacattcca cactttcatc atgccgcgct tttcctgcat cattacggag tgcaaaaacg
      121 cagtgccggt gatccatggc agcagagagg cattctcgac aggatcccac gcccagtagc
      181 ccccccagcc gagcacggcg tatgcccagt gcgcgcccag cagaatgccc acagttagaa
      241 ggccccacgc aaccattgtc cagcgccgcg tgatgtgaat ccacttttcg ccgggatagc
      301 ggccaagcaa tgccgccatg gcgaaagcaa acgggacagt aaatcccgta tatccgagat
      361 agagcatcgg cggatggatc accatctcgg gatattgcag aagcggattc agtccgctgc
      421 cctcggaaac tcttgccata tgcatcgcgc ccgatgcatc ggcgccagcc aggatgtgaa
      481 atgggctcgc cacgaaaacg ttcagaatca gaaagaaaat ctggacgccg gccagcgcca
      541 ctcccacgta cggcatcagt tccgggtgtt ttttgcgatt caagaaaagc gcgcagaacg
      601 catagattga gagcagccag ctccagaaaa gcaaggagcc ttcctgtcct gaccaaagcg
      661 cggcgaattt ataaaacaag ggcagagcgc gattactgtg ctctgccacg taggccaggg
      721 agaaattgtc ggtgaaaaag aagtattcaa gcgcggcgac tgcaagcgta acaaggccgc
      781 agaccgccat cccggcattg cgcgcgcttt tcgaaagcaa cgtgcgcctt gtgaggatac
      841 cggttatgcc cgctacaaag gcgtacgcgg cggcaagtag tgcgaggaga agtgcaaacg
      901 aaccaaaaat gtccaacgag tttccccctg ccgaatctag cttttgcgcc ctgacgtcgg
      961 cacgcttggt tcggtcgtcg attggtcaac aggtggagcg gagtgtttcg cgcccgccat
     1021 gccaggtggc gtcgcttcat acttggatgc gcattttgcc tgtactcctt cggcaacaaa
     1081 tccgccctgg ggcatcagcc tgccttcaac gagcgcctgt gcgccgtcct tgaaagtatc
     1141 gggaagggga tcccggccaa cgtagctgac cggcaagtcc ttgccctgct cctctaggac
     1201 aaaattgatg tggccgtcgg catgtgaaat cgacccggca cggacattgc ccgaaactcg
     1261 catgcgctga tggagcgccg gagcagagag catgggcagt tcggagatcg tgtggtagta
     1321 ggttttgctt tgcgtgaaac cgacgtaagc cagcactaaa agtgtggcga cgatgattcc
     1381 cgaaccagtc agaaatttcc cacgggcctt catgcttgca ctcccactct atgattcccg
     1441 aaagatgaat tctaacacag cgtctgattg cgaagggagg tttgaacggc tggggcgact
     1501 cttttgggga tgatccaggc agtcgcgtcc gttcttgtcc ctgctagagt gcgagcagag
     1561 agaaatccat atctaagctg gcgccattcg gccaactaaa gaggtacggc gacgggctca
     1621 acttcgacgt aattgcgccc gcggtgcttt gctcgataaa gcgctgcgtc ggctcgggtg
     1681 accaactcgg agaagtcggg agcatttttg cccaaaaatc cggccacgcc gaaacttgcc
     1741 gtcacgacga attcggctcc attgacaagg aatttttcgg caccaaactg gctgcgaatg
     1801 cgctcaagca ccaatgcaac acttgcttca tccgcgtgag tgatcacttg aaggaattcc
     1861 tcgccgccta tgcggcccga gatgtcagac aaccgcgtat ttgatttgag gatttcagcg
     1921 aatttccgga gaaccatgtc tccggcatcg tggccgtgtg cgtcgttcac gagcttgaag
     1981 tgatcgagat cgatcatcac aacccagaaa ggaaagccgt gccgtgcggc tccgctcagc
     2041 tggcgggtcg cccaggtttc aatcgcgcgt cggttgggta gaccggttag cgcgtcggtg
     2101 agcgccagtt cttcaagaag tcggctcttc gtcttgatct ggcggtgcag gccaatcagc
     2161 ctctggccca cacggacgcg cgccaaaagt tcggcggcat gaaatggttt agtgaggtaa
     2221 tcatcggccc ccgcagaaag gcccatcacc acattttctt tttcagcgtt gctggtgagg
     2281 atgattacgt agatgtacga agcgtaggtg ccttcacgga tgcgctggca aagctcgatg
     2341 cctgtaagat cgggcatcag ccaatcagtg atgatgattt caggatgttc cagttcgaag
     2401 atttcgagag cttgatgacc gctcgtagcg aacagaaccg cagaggagtc ttgggaaagc
     2461 gcatgctcaa ccagcttacg atagacaggt gagtcgtcga cgaccaagac tttgaagccg
     2521 ctcatctaag cccctgctgc gtacctcaga tcttccgagt atcgctgggg ccgtccgcga
     2581 cttccgggct ggccgcttgt gtttggccga gcttggcgcg caagcagccg atcaattccg
     2641 gcgcgagatt ggaaatttcc ataccttccg agacaatgaa tttgcagctc agaatcgggc
     2701 gcgccgcatc gtcatcgggg agtttgcagt caacgcggca atattggcat tcctgattcc
     2761 agcagaatcg tccgtaggga atggtttccg ggctgatgaa ctggaaagcg cggagcaagg
     2821 aattcctctc gggcaccaga aaagtttttc ccaggattgt gatttctacc agccgttcgt
     2881 acggcctgaa tacgtctgcc atgagaggta cttcttgttc cgccatttag tgattccccc
     2941 gcggatttac gctagttcat ggcctcgcaa cgagcaatga gaactaagtt tttcggtgtt
     3001 caataccgca acgggaagaa aaacacggta ccgaagcagg tcgactttgg cgtttgctaa
     3061 gcggcggtag gaaggcgttc gtgtgttcgc caagaagcgc ggggaaccag caaaaccagt
     3121 gtgtacacac ctagggcaat accgagggga ccgttaagga ttcctaggaa actagaggtc
     3181 agcgccagcg tgcgactcga ggacccgcgt ttcatcagtg caaatcctgc cagaagcgag
     3241 aaaataccgg tcacaatagc cagcgcaatt attcccacca gaaaaaaacg aaagacgctc
     3301 atccaagaaa agggattggc cacacgcgaa acaatcgtgc cccacataat cgtgagggtc
     3361 gcaacattca gcgcaaccca cacagcctcg gccaggcaga ttaaaccata gatcgtccac
     3421 agggccccta atctaccctg cggtttatct attggcctca tcgaccagcc cgctgccgga
     3481 ccggagattg cgaatatcat ttcgagaaga tgcgctcccg cgccgctatc tgcaatacga
     3541 tgaaaactgt atatttttgt gggtgttagg cgaagtattg gccgatgctg cgaaactttt
     3601 catatcgctg ctggagcagt ttttccggag gcacaagcaa aagctgattg agtgcgcgtg
     3661 cgagaacgtc cccgacgagt ttggcgcagg caggatggtc catgtgagcg ccgccctcgg
     3721 gttcgggaac aatttcatcg atcagccgca gatcgagcag atcgcgcgct gtgatcttta
     3781 atgcgtcggc ggccatttcc gattttgttg gatcgcgcca catgatggac gcgcagccct
     3841 cgggagaaat caccgagtac acagaattct ccagcatgaa aatacggtcg ccgatagcaa
     3901 tcgccagcgc cccgccggaa ccgccctcgc cagtaatcgt gaccagaatc ggcaccggca
     3961 gtcgggcaat ttcacgaatg ttgtaagcga tggcttcggc ttggccccgt tcctcggcgt
     4021 ctatcccagg atatgcacct tgcgtgtcca caaaaacaaa aatgggacgc ccgaatttcg
     4081 cggccagatg catggcgcgc agagctttgc ggtacccttc gggcttggcc tggccaaagt
     4141 tacgagccag gcgttgcttt gtatcacgtc ctttttggtt gcccactacc aggacagagc
     4201 ggccctgaaa gcgagccatg cccgtgatta aggcgggatc gtcagcgaag tagcggtcgc
     4261 cgtgaacctc gctgaagtcc tcaaacaaga ggcgtatgaa atcgtcggtg taagggcgct
     4321 gaggatgccg ggctagctgg agacgccgcc atgaatccag gtgcgagtaa aactcgcgct
     4381 ttagctcctc aacttcgcgc tgaatacggc cgatctcttc cgcagtagta tcggtagggt
     4441 cggtcaggcg gctgagttcg ctaatatcgc gttcgagtgc gtcaatttct aactggcgct
     4501 gtgcgttaac gtctttcacg ctggcgttat ccgcagagga tctcgtgctc tgcgcggcac
     4561 ctctttccgc ttgaagcttg cgcctcttcg ccaatgcgcg catccggcgc aaacgcttgc
     4621 gcgcgagctt gcgcttttcc agagaatcgg tcattgcatc accgcaacag aatcggctcc
     4681 gcaaatctcg cggacgcggt tgagaaactc ggaatcggcg cgcacggcac tgcccgcatc
     4741 aatcgtggcc tccgtgccgt cgtcgtggac cagctcgaat gcaatgcggc atcgccccgg
     4801 cctgctggcg aacagttcct gcagtcgatc gatgatcccg gcgtctatgg cgctgcgatt
     4861 cacgcggaca cgcaacaagt ttggcgcgct gggctgctgg cgcacctgat ccagaagccg
     4921 cgcatcggaa atgatcagtc gcgtcccaac gtcctccacg gcgatccgtc ccttcatcag
     4981 caatggcgtt gcgcctttta gaattgcctc gagttttaga aatgcctccg ggaatgccag
     5041 cgcctcgcac acgccggttc ggtcttgcaa agttaggatc gcccaacggg cgcctcgccg
     5101 cgaccgcatc gcgcgggact gcacgataat ccccgctacc gtaacttcct ccccgttgcg
     5161 tcggccttcc agactcgcga gttcggccgc cttcatctcc tgcagtcgcc ctgtgtattt
     5221 gtcgagtgga tgtccggaga tgtaaaagcc gagcgtcgag tattcggctg ccagcagctc
     5281 gtgctcggcc cattctgcgg cgtcgggcaa aatttccggt cggggcgttt cagtcaccgc
     5341 ggttccacta ccaaaaaggc cgtgctggcc ggactcgcgc gtccgctgga ttttctgcgc
     5401 gcgctcgacc gcgcggtcaa tgcccgcgat catctgcgct cgatgagcgc ctagaccgtc
     5461 gagtgcgccg gctctcgcaa ggctctccag aacgcgttta ttgatcaatc gaggatccac
     5521 cgattcgcag aactggaaaa aactggtgaa ttgtccgagg gatttccgcg ccgccaaaat
     5581 tccttttacc gtgttctcgc ccacattttt tatagcggcc aggccaaagc ggatatctcc
     5641 gccgaccgga gtgaaattca tatcactcga attgacattg ggcggaagca cagttattcc
     5701 catgctgcgc gcttcgttga tgtacttcac cactttttcg gtatttcccg tttccgaagt
     5761 cagcatcgcg gccacgaatt ccaccggata atgtgccttg agccacgccg tctggtaagc
     5821 cagtagcgca taggcgcacg agtgtgactt attgaagccg tagcccgcga attcagccat
     5881 gagatcgaaa atcttttcgg cctttttctg cggcactttg cgcgcgtggc agccagtcag
     5941 gaatttctca cgctgcgcaa ccatctcctc gtgttttttc ttacccatcg cacggcggag
     6001 caaatcggca tcgcccagcg aaaatcctgc cagacgattc gcgatttgca tcacctgctc
     6061 ttgatagagg attacgcccc acgtctcgga aaggatctcc tcaagttccg gcaaatcgta
     6121 tgtgaccttt ttctttccat gtttgcgggc gatgaaatcg tcaatcatcc ctccctggat
     6181 gggaccagga cggtacagcg cgttgagggc cgttaggtcc tcaatccgag acggctggta
     6241 gcgcctcaaa atgtcccgca taccgtgcga ttcgaattgg aagatgcctg tcgtgtcgcc
     6301 gcgcgaaaat agtgcgtaca cggcgggatc atccagcggc aacgtcgcta gatcgacgga
     6361 aacgccgcga ttctgctgaa tcaaccgggc cgcatcatcc aggactgtca atgtcgtcag
     6421 ccccagaaaa tccattttca gcaggccgag ccgctcgagc gcgttcatgt cgtactgcgt
     6481 ggttacttca tctttattcg ttttatacag tggcacgatc tcggtgagcg gccgcggcga
     6541 gatcactact ccggcagcgt gtgtggacgc atgccgtgcc agtccctcga gtcgcagcgc
     6601 cacctcaacc atctccctca cgcgttcgtc cgacttgcgc aatgaatcca gttgaggagt
     6661 ctgcttaagc gcatcctcca attcgatatt cagcgtcgcg ggaatcatct tcgcgatttt
     6721 atccacctct ccatagggca tgtcgatagc gcggcccacg tccttcagcg ccgccttcgc
     6781 cgccatggtc ccaaaggtga tgatctgcgc aacgctcccc gcgccatacg tttgcctgac
     6841 atattcgatc acttcgccgc gccgccgcat gcagaaatcg atgtcgatat cgggcatcga
     6901 aatgcgctcg ggattaagaa agcgctcaaa cagcagatcg tattgcaaag gatcgacgtc
     6961 cgtgatctgt agcgcgaagc tcaccaggct acccgccgcg gaacctcgcc ccggccccac
     7021 gggaattccc tgcgatcgtg cgtaacggat gaaatcccac acgatcagaa agtagcccgc
     7081 gaagcgcatc ttctgaatca tttgaatttc ggcaaataag cggctctcgt aatccgaaag
     7141 cgaatggcgc aggcgattct ctttcgccag gcgttccaat tgctgggcgc gggcggcaaa
     7201 cccctcgcgc acaacgcgct caaaatacga atcggtggta tggccctcgg gaactttgaa
     7261 ctccggaaac gggttgctga cgcgctggat tttcacgttg cagcggtcgg caatcgccac
     7321 cgtgcgcgaa acggcatccg gcagctccct aaaaacttgc gccatttcct cggcggtctt
     7381 gaaataaaat tggtcggtcg cgaacttcat ccgctgggcg tcgctcatgg tcttgccggt
     7441 ctgaatgcac atcagcactt cctgcgcatg ggaatccgat tgggtcaggt aatgacaatc
     7501 gttcgtggcc actagcggaa tccccgtttc gcgcgaaaga gcgaccaagt ctcgattgac
     7561 gcgcgtctct acatccaaac cttggtcctg cacctccagg aaaaaattac ccttaccgaa
     7621 aatatcgcgc agacggtatg cgttcgtccg cgcctgctcg tatttttcgc tgagcagggc
     7681 atcggccact tcgccgcgaa ggcacgcgga aagcgcgatt aggcccttgc tatgccgagc
     7741 cagaaggtca tggtcgatgc gcggcttata gtaaaagccg tcgagaaacc cggttgatac
     7801 cagtttaatg aggttgcgat agccttcgtc gttttcgcac agcaagataa gatgattcga
     7861 tcgttccgcc tccgcgccgc gatttttgtg attgtccggc gctacgtaga cttcacatcc
     7921 gatgatcggt ttcaccccgt gcgtggtggc ttgatgataa aagtttgcgg cggcgaaaag
     7981 attgccgtga tccgtcaccg caaccgcggg cattccgcgt cgcgccgcct ccgccgtcag
     8041 ttcgccgatt tcgcaggcgc catccagaag agagtagtcc gtgtgcagat gcagatgtac
     8101 gaagttggaa gaagctgagg aagccatgca gacctattct aatccagctt gcaagcgcgc
     8161 gatctgattt tctcgctgcc ttctgtgccg atgcgccgct ctcagtttcg aagaatattt
     8221 cgagggcggt gctgcaactt gcaactaatg accgtctcga gtgttctaac gatagaggcg
     8281 cgagcgtgcc cttgcagtgg ccgaggctgt tttgaactgt cgacgattga agaggtgagc
     8341 caatgcactc agcaagacta attccaataa gccttttatt ggttgcctgc gcggtatcga
     8401 ccgccaacgc gcaatggagc tctaccgaac ctcatcgtaa tttcgaaatc acgccgttcg
     8461 gaggatcacg ttttggcggt gtgatcaata caagttcagg cgggtccaac gttattgacg
     8521 aattcacgat caaaagcaca tgggactacg gcgccatcgc cgatatcgac ttgctgcccg
     8581 actccctgcc tggccttcag ggcgagttta tgtggagtca ggagcctacc acccttggtg
     8641 cccgcgattc cctaaccggg atcatttcgc ccgctggcaa gctcgagctg aacaattatc
     8701 aatggggcat cgattacaat ctcggcagtc ggttctcgaa actacacccc tttgttgtcg
     8761 gcggtatggg tttcactcat tttagctcgg gaggcgtgct gcccttcgag aacaagctct
     8821 cttttaatct cggcggcggc gtgaagtatt ttgtcgtccg gcatgccggc gtgcgcttcg
     8881 acgtgcgctg ggtgccgtca cgcaccactt cgcatgtcga gacgtttttc gatccgtttt
     8941 tcgggttctt ccaagcaaac gttaacaatt acgccgagca gtttcaggcc aatctcggct
     9001 tgattttccg attctagcgt catgaggatg ctcgcgcgga tggtcagaaa tcgtcggact
     9061 gctgagcgaa ggtgtagccg ggcagattcg ccgcgggtcc atttttggcc actttgatgc
     9121 ggattggagg ctcgccttcc gcccccgcct tgcgcaagac ggcatacgtc gccagcgcct
     9181 cgctgtagat cttagtgatc agaaaggaat cgtcggttcc gtccttcttc caaacctggc
     9241 caacttgtac ctttcccgtg ggcacgatac gaatcgtaga cagattcaag gtatgcgtca
     9301 actcggtatg cgggagctcg tgcggaaatg aatgcctctc gagcacgaag ctgttttgcg
     9361 cgcggcggct tcttgtggca tcttagtctg gtagggaagc gatgcccgca gagaagaaaa
     9421 tcgcggttgt ttgtttccag ttgggaggac ccgaccggct gagcgccgtg gagcccttcc
     9481 tctacaatct cttttgcgat cctgacatca tcgattttcc aggctcgttt ctcgcgcgca
     9541 aaccgctggc gaaattgatc tcgaccacgc ggtcaaagac cgtccgccaa cattacgccg
     9601 aaatcggcgg cggctcgccc attcgccgcc tcacggagca gcaagcgcgc gctctggata
     9661 tggccctccg cccccatatt tccgcgcaca cattcgtggc catgcgctac tggcatccga
     9721 ataccgaaga agcgattgcg gctttgcagc gcgtctcctt cgacgaactc gtgctgctgc
     9781 ccctctatcc gcattactcc ctggcgaccg cgggcagcag cctaaaggaa tggaaccgcc
     9841 tttacgctcc gcacgttccc gtgcatgtgg tcgatcactt tttcgatcat cctgattaca
     9901 tcgctggagt cgtcgagcgc atcaacggga tacttcagca gttgccgtat cccgacgaag
     9961 tgcccttagt gttcagcgcg cacggacttc cgatggcgct ggtggaaaaa ggcgaccctt
    10021 acccgcgcca gattgaggcc acagttcatt tggttcgtga gctcggggcc tgggccaatc
    10081 cctacgtgct ttgctttcag agcaaggtcg gccctcagaa atggctgcag ccctcgctca
    10141 ctggcaccat tgcgaaaatg gctggcgaag gtatcaagcg gatgctcgtg gttcctattt
    10201 ccttcctcac ggagcatatc gaaaccctct acgagatcaa catcgaagcc cgggaacagg
    10261 ccgagtctct cggtgtgagc gattttcaca tgatgcccgc gctcaacgat tcgcccctcc
    10321 tgatccgcgc ccttgctgat ctcaccctac gcgcggtagg ccgccgcgcc ggcgtcgcct
    10381 ccctcgcttt gtagcggcgt cctaagcgaa gccgaaggcc atctcgcctt tgctgtcatt
    10441 ggcttgtcct ccatgctctg cgcctctgcg gtgagcctct ttgcttttgc cttgttaacg
    10501 ggtccaccct tggctttggc cccagccccg cccagggcgc catgatataa acgtattgtg
    10561 gaattctctc tcaccgacga acagcagcac ctccgccgca ccgtccgcga attcgccgag
    10621 gcggaaatcg ccccgcatct gatggagtgg gacgaagcct cccaattccc ctcggaaatt
    10681 attcccaagc tcgccgagat gggctttctc ggcgtcattt tcccggaaaa atatggcggc
    10741 gcgggcatgg gctatctcga atacgccatt atcatcgagg agctttcccg cgtagatggc
    10801 tcggttggca tcatcgtcgc cgcccacaat tcgctctgca ccaatcacat ctacaaattc
    10861 ggcaccgagg agcaaaagca gaaatacgta tcgccactgg cgcaaggcaa gaaactcggc
    10921 tgctggtcgc tcactgaacc ggaagccgga tcggacgcag gcggcacacg caccgtggcc
    10981 gacaaaaaaa acggcggctg ggtactgaat ggcgcgaaga cgttcaccac caacggtcac
    11041 tatgctgacg tctgcgtcgc gatggccgtc acggacgctt ccaagaagca ccacggcatt
    11101 tccgcattca tcattgagaa gggaacttcg ggctttcgtc ccgggaaaaa agaaaataaa
    11161 ctcggcttgc gcgcgagcga taccagcgaa gtggtcttca ccgattgccg cgtgcctccg
    11221 gaaaatcttc tcggtcccga gggcgagggc ttcgtcaaca gcctccaaat cctcgatggc
    11281 ggccgcattt cgattgcgtc gctcggcctc ggcatggcgc agggcgcgta tgagtgctcg
    11341 gtgcgctacg ccaaggaacg caaacagttc ggcaagccca tc
//
LOCUS       DATNDT010000195         7398 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1562922, whole genome shotgun sequence.
ACCESSION   DATNDT010000195 DATNDT010000000
VERSION     DATNDT010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1562922"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VN788_06380"
     CDS             <1..781
                     /locus_tag="VN788_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="succinate dehydrogenase"
                     /protein_id="HXU20189.1"
                     /translation="VRGCTTRVSESSTAGGLTRFGSTQRRDAWWIENLPFIIVLGAFT
                     VYTTWRAFAGDFYWADPYLSPFYSPLIDPQHHWWPFSPALLILGGPLGFRATCYYYRK
                     AYYRAFFLDPPGCAVGESSGSKYRGETAFPFILQNVHRYFFYIAVIFVAFLWYDAIIA
                     FDFDGKFGIGVGTLVMLVNIILLTIYTFSCHSLRHLMGGKLDCFSCATLGAPRESVWR
                     GITKLNEHHMFWAWVSMISVGLTDLYIRLVASGALKDIRIL"
     gene            778..2496
                     /locus_tag="VN788_06385"
     CDS             778..2496
                     /locus_tag="VN788_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HXU20190.1"
                     /translation="MTSKYETHEHDVLIIGAGGAGLRAAIEALAHGARVAVVCKSLLG
                     KAHTVMAEGGLAAAMANVDKADDWRTHFRDTMRGGKLLNNWRMAQLHAQEAPDRVREL
                     EQWGALFDRTEKGEILQRAFGGHTFKRLCHVGDRTGLEMIRTLQDRGVEMGFDVFMEC
                     TITRLLKDGDRVCGAFGYWRESGNFVTFKAKSVVIATGGIGKSWKITSNSWECTGDGM
                     ALAYQAGAELLDMEFVQFHPTGMVWPPGVQGLLVTEAVRGEGGILRNKNGERFMEKYD
                     PKRMELSTRDVVSRSIYTEVREGRGTPHGGAFLDISHKPAEYVKRKLPSMYHQFKELA
                     DVDITKGPMEVGPTCHYVMGGIRVDAETGRSSVPGLFAAGEAAGGLHGANRLGGNSLS
                     DLLVFGRRAGLAAAEQAQKTPAPPINDEQAQDAERQALAPFERSGGENPYTIHHDLQE
                     TMQNLVGIFRTDEDLRKALVEIEKLKERAKKTHVEGTRLFNPGWHLCFDLQSMLTVSE
                     ACTHAALARKESRGAHSRLDFPKTEDEWGKKNHVIVKKGDTMSLFEVPKPDLPADLQQ
                     VLAEDK"
     gene            2523..3257
                     /locus_tag="VN788_06390"
     CDS             2523..3257
                     /locus_tag="VN788_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HXU20191.1"
                     /translation="MADATLRVFRGNKEGGQAVDYKVPVTPGMVVLDAVHSIQAHQAP
                     DLAVRWNCKAAKCGSCSAEVNGKPRLMCKTRMDSLPLDQPIIVYPMKAFPIIKDLVTD
                     VSWNYKVNKKIPAFKPRAGATWKFYQRDVDRVQEFRKCIECFLCQNVCHVLREHDKKD
                     VFGGPRFFVRTAGLEMHPMDGVDRIPLLKEQLGIGYCNITKCCTEVCPEEIHITDNAI
                     IPLKERVVDQFYDPLTMIVRKISGSK"
     gene            3312..3725
                     /locus_tag="VN788_06395"
     CDS             3312..3725
                     /locus_tag="VN788_06395"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HXU20192.1"
                     /translation="MRTVVAAVIEHEGKLLACQRARGCRSELMWEFPGGKVEPWETLP
                     EALSRELREELGVTAQIGPEVFHTRHTYADTDEPLDLFFFAAHASPAEIQNLEFERIE
                     WCTPESLPNLAFLAADRPLIDALATGAVRVPRQSA"
     gene            3808..4653
                     /locus_tag="VN788_06400"
     CDS             3808..4653
                     /locus_tag="VN788_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HXU20193.1"
                     /translation="MKKFESFKAANAEEAALLEKLDPRRLPKHVAAIMDGNGRWAQRR
                     HLPRIAGHRAGVKASRVVIETCAQLHISCLTLYAFSLENWRRPQPEVDFLMRLLREYL
                     KRELPLIQQNNIQLLAIGRSNQLPETVRKDLSDAMRQTAHNTGMKLVLALNYGGRAEL
                     ADAFNAMLDHVRANGLAAFHADEQSISDHLYTAGLPDPDLLIRTSGEMRVSNFLLWQI
                     AYAEMYVTETLWPDFSRARLLEALVDFQDRERRYGGLESARGAAPPHKKDAPGMGRIA
                     RTGRG"
     gene            4660..5520
                     /locus_tag="VN788_06405"
     CDS             4660..5520
                     /locus_tag="VN788_06405"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HXU20194.1"
                     /translation="MLLRILTAAGLIPAVVALVWWGPAPLLVAVAAAVAILALREFFN
                     LGELVGLRAYRYWTFVCAVGIFYAQYVAGSADTLTLGSGVTIVRPSSNPLAISLDEVL
                     LVFVFGLMIAGLATRRPLQDVLSAVSISSAGLLLISLPFSYLVRINEIVPGGRDLVLF
                     TLFLIWAGDMLAYFTGRTVGRTPFAPILSPKKTWEGAIANFVASLLVAVAFARWVQLD
                     VVALAAIAALANIAGQMGDLIKSAYKRAASVKDSGTLLPGHGGMLDRIDSLILASPVV
                     WLAWHMLAPK"
     gene            complement(5799..6191)
                     /locus_tag="VN788_06410"
     CDS             complement(5799..6191)
                     /locus_tag="VN788_06410"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HXU20195.1"
                     /translation="MPIPIDTRKKPVDVKVYVSTGEGVAITWSDGHTSRYDFPYLREN
                     CPCATCNDERAKREADPKAAAAAAVLPMYKPRVTAKAAKAVGNYAIQIDFSDAHATGI
                     YSFGCLRELCPCDECAREFRAAGKKAAS"
     gene            complement(6404..7249)
                     /locus_tag="VN788_06415"
     CDS             complement(6404..7249)
                     /locus_tag="VN788_06415"
                     /inference="COORDINATES: protein motif:HMM:NF018983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016977 - chitosanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 75 protein"
                     /protein_id="HXU20196.1"
                     /translation="MLVLMQRIGRIFPLVVALALPASGLAGNIHAAAPNDAVDSLTSC
                     PTRSLLLNFDVYRGEQAVRQVPIWRLQGNAAFFFTSGLMIDADGAPNAYSPDDTGLDD
                     LSNAGQPGHWDGIIADDEGNPVVQGPGDPFPGYYISCTALSDRTKERIDPTRYVDASK
                     IPYIALPGELARDSGARLGDLAVVVNTRNGRFSYAIFADIGRLGEGSIALADNLGIWS
                     DARHGGRWAGIQYLVFPGSGDDRPMSLEEINERTETLFHDWGGIDHVRSCADHAPEKP
                     PFWYE"
BASE COUNT         1465 a         2156 c         2158 g         1619 t
ORIGIN      
        1 agttcggggg tgcacaacta gggtgtcaga gtcttcaacc gcagggggac tgactcgttt
       61 tgggagcacg cagcggcgag acgcgtggtg gatcgagaac ctaccgttca tcatcgtcct
      121 cggcgctttc actgtttaca ccacgtggcg ggcctttgcc ggcgactttt attgggccga
      181 cccgtacctt tcgccgttct attccccgct aatcgatcca caacatcatt ggtggccgtt
      241 ctcgcccgcg cttttgattt tgggcggccc tctcggcttt cgcgcgactt gctactacta
      301 ccggaaagcc tactatcgcg cgtttttctt agacccgccc gggtgcgccg taggggagag
      361 tagcggcagc aaataccgcg gcgagaccgc tttcccattt atcctccaaa acgttcatcg
      421 ctatttcttc tatatcgcgg ttatttttgt ggcgtttctc tggtatgacg cgatcattgc
      481 tttcgatttc gacgggaaat tcggcatcgg cgtcggcacg ctggtcatgc tcgtcaatat
      541 tattctgctc acgatttaca cgttctcgtg ccattcgctg cgtcatctga tgggcggcaa
      601 actcgattgc ttctcttgcg cgactcttgg ggcgccacgc gagtcggttt ggcgcggaat
      661 caccaaattg aacgagcacc atatgttctg ggcgtgggtg agcatgattt ccgttggcct
      721 cactgacctc tatatccgct tggtggcctc tggcgcgttg aaggatattc gaatcctgtg
      781 acctccaagt acgaaactca cgaacacgac gtgctcatta ttggtgcggg cggcgcggga
      841 ctcagagctg caatcgaggc gctggcgcat ggcgcgcgcg tggcggtggt ctgcaaatcg
      901 ctgctcggca aggcgcacac cgtgatggcc gagggcgggc tcgccgccgc aatggccaac
      961 gtcgataaag cggacgattg gcgcacacat ttccgagaca ccatgcgtgg gggaaaattg
     1021 ctcaacaatt ggcgcatggc ccaactccac gctcaggaag ctcccgatcg ggtccgcgaa
     1081 ctcgagcagt ggggggcgct ttttgaccgg acggaaaaag gtgaaattct acagcgcgct
     1141 tttggcggcc acacgttcaa gcgcctctgt cacgtcggcg atcgcaccgg cctcgaaatg
     1201 atccgcacct tgcaggaccg cggcgtcgag atgggcttcg acgttttcat ggaatgtacg
     1261 atcacgcggc tactcaagga cggcgaccgc gtctgcggcg cgttcggcta ctggcgcgaa
     1321 agcggcaact tcgtcacctt caaggctaag tccgtggtga tcgctaccgg cggcatcgga
     1381 aaatcctgga aaattacctc gaattcttgg gagtgtacgg gcgacggcat ggccctcgcc
     1441 taccaagcgg gcgccgaact cctcgacatg gaattcgtgc aatttcatcc caccggaatg
     1501 gtctggccgc cgggagtgca ggggcttctc gtcaccgaag cagtacgcgg cgagggcgga
     1561 atcctgcgca acaaaaatgg cgagcgcttc atggaaaagt acgatcccaa gcgcatggag
     1621 ctgtccacgc gcgacgtagt gtcgcgctcg atttacaccg aggttcgcga aggacgcggc
     1681 actcctcacg gcggcgcatt cctcgatatt tcgcacaagc ccgccgaata tgtgaagcgc
     1741 aagctgccaa gcatgtacca ccagttcaag gaacttgcgg atgtggacat caccaaggga
     1801 ccgatggaag tcggtcctac gtgtcattac gtgatgggcg gcattcgggt ggatgcagaa
     1861 actggacgat cctctgtgcc cggacttttt gccgctggcg aagcggccgg tggccttcac
     1921 ggcgcgaatc gcctgggagg gaattctctt tcggacctgc tggtattcgg tcgccgcgcg
     1981 ggcttggccg ctgctgaaca ggcccagaaa acgcccgcgc cgccaattaa tgacgagcaa
     2041 gctcaggacg cggagcgcca ggccttggcc ccctttgagc gctccggcgg tgaaaatccc
     2101 tatactatcc atcatgacct gcaagagacg atgcagaatt tagtcggaat tttccgcacg
     2161 gacgaagatc tgcggaaggc gctcgtcgaa atcgaaaagc tgaaagagcg agccaagaag
     2221 acgcacgtcg agggaacgcg cctgttcaat cccggctggc atctttgttt cgatttgcaa
     2281 tccatgctga ccgtttccga ggcttgcacg cacgcggcgc tcgcccgcaa ggaaagccgc
     2341 ggcgcgcaca gccgtctcga ttttccaaaa accgaagacg aatggggcaa aaagaatcat
     2401 gtaatcgtga agaagggcga tacgatgtcg ctcttcgaag tacccaagcc ggatctgccc
     2461 gctgatttgc agcaggtttt ggcggaagat aaatagcaag gagatagcgc ggagggtttt
     2521 tgatggcgga cgcaacttta agggtttttc gcggcaataa agagggcggc caagcagtcg
     2581 attacaaggt gccggtcacg ccgggcatgg tcgttcttga cgccgtccat tccattcagg
     2641 cgcaccaggc tcccgacctt gcggtgcgtt ggaattgtaa ggctgcgaag tgcggttcgt
     2701 gttcggcgga ggtaaacgga aagccgcgtc tgatgtgcaa gacgcgcatg gattctttgc
     2761 cgctagatca gcccataatc gtatatccga tgaaagcctt tccgatcatt aaagacctgg
     2821 ttaccgacgt ttcctggaac tacaaggtca ataaaaagat tccggcattc aagccgagag
     2881 ctggcgcgac gtggaagttt tatcaacgcg atgtggaccg cgtgcaggaa ttccgcaagt
     2941 gtattgaatg tttcctttgc cagaatgttt gccacgtcct tcgcgagcac gacaaaaagg
     3001 acgtgttcgg cggcccgcgc tttttcgtcc gcaccgcggg tctcgaaatg catccgatgg
     3061 atggcgtgga ccgcatcccg ctgttaaaag aacagctcgg catcggctat tgcaatatca
     3121 ccaagtgctg cacggaagtc tgccccgaag aaatccacat cactgacaac gccatcattc
     3181 ccctcaagga gcgcgtcgta gatcaattct acgatccgct aaccatgatc gttcgcaaaa
     3241 tctccggctc caagtaatcg tttcttcgcc atcgcggcgc accgcgttat gatgagattg
     3301 aagggatatc gatgcgtacc gttgtagctg cggttatcga acatgagggc aagttgctgg
     3361 cttgccagcg ggcgcgcggc tgccgttccg agctgatgtg ggagtttccc ggcggaaaag
     3421 tggagccgtg ggaaactttg ccggaagctc tctcgcggga attgcgggaa gaactcggcg
     3481 tgaccgccca gataggcccg gaagtttttc acacgcgcca cacttatgcg gacaccgatg
     3541 agccgctcga tctcttcttc ttcgcggcac atgcctcgcc cgccgaaatc caaaatttgg
     3601 agtttgagag gatcgagtgg tgcaccccgg aatcgctgcc caatttagct tttttggctg
     3661 cggaccggcc tctgatcgac gccctggcaa cgggcgcggt ccgggtgccc cgccagtcgg
     3721 cgtagcccta gcggcggccc ggctgcggtt gcggacggat agtcgctgtg ctactttcgc
     3781 tcgcgggcgt ccttgaactt atctcccgtg aaaaagttcg aatctttcaa agccgcgaat
     3841 gctgaagaag ccgcgcttct agaaaagctg gaccctcgca ggcttcccaa acacgtcgcg
     3901 gccatcatgg acgggaacgg gcgctgggcg cagcgccgcc atttgccgcg gatcgccggg
     3961 catcgcgcgg gcgtaaaagc ttcccgcgtg gtgattgaaa catgcgcgca attgcacatt
     4021 tcgtgcctta cgctgtacgc tttttcgctc gaaaactggc ggcggccaca gcccgaagtt
     4081 gattttctga tgcgcttgtt gcgcgaatat ctgaagcgtg agctgcctct gatccagcag
     4141 aataatattc aattgttggc gatcggtcga tccaatcaac ttccggaaac cgtgcggaag
     4201 gacctctcgg acgccatgcg ccaaacggcg cacaacaccg gaatgaaact cgttctcgcg
     4261 cttaattacg gcggccgggc cgagctcgcc gacgccttca atgccatgct cgaccatgtc
     4321 cgcgccaacg gtcttgccgc cttccacgcg gacgagcagt cgatttccga ccatctgtac
     4381 accgccggat tgcccgatcc ggatctcttg atccgcacca gcggcgaaat gcgcgtgagc
     4441 aattttctgc tttggcagat tgcctacgct gaaatgtacg taaccgagac gctctggccc
     4501 gatttctcgc gcgcgcgttt gctcgaggcg ctcgtggatt ttcaggatcg agagcgccgc
     4561 tacggagggt tggaatccgc gcgcggcgcc gcgccaccgc ataagaagga tgctcccggc
     4621 atgggacgca tcgcacgcac gggccggggc tgacgccgga tgcttcttcg catcttgacc
     4681 gctgcggggc tgataccagc cgttgtcgcg ctggtgtggt ggggcccggc gccgctgctg
     4741 gttgcggtag cagccgcggt tgcaattctt gcactgcgcg agtttttcaa tttgggcgaa
     4801 ctcgtcggct tgcgggcgta tcgctattgg acattcgtgt gcgccgtcgg aatattctac
     4861 gcgcagtatg tggctggttc ggcggacaca ttaacgctcg gctccggagt cacaatcgtt
     4921 cggccgtcat cgaatccgct tgccatttcc ctcgatgaag tgctcctcgt tttcgttttt
     4981 ggtctgatga tcgcgggtct cgccacgcgc cggccgttgc aggatgtgct ctccgcggta
     5041 tcgatcagct cggcgggact tctgctgatc tcgctaccat tcagctattt ggtgcgcatc
     5101 aacgaaatcg tccccggcgg ccgcgatctc gttctattta cgctttttct gatttgggcc
     5161 ggcgacatgc tggcctactt taccggccga actgtcgggc gcacaccatt cgcgccaata
     5221 ttaagcccaa agaaaacgtg ggaaggcgcg atagcaaatt ttgtggcttc gctgctggtt
     5281 gccgtggcct tcgcgcgatg ggtgcagttg gacgtcgttg ccctcgcagc tatcgcggcg
     5341 ctcgcgaaca tcgccggtca aatgggtgat ctgatcaagt cggcttataa gcgcgccgct
     5401 tccgtcaagg attcgggcac gcttctgccc gggcacggcg gcatgctgga ccgcattgac
     5461 agcttgattc tcgccagccc ggtcgtctgg ctagcctggc acatgctagc cccgaagtag
     5521 cgaggcgctg ccagcccggt atcgtggttt tgtagaggcg ctgccttgcc ccgagcggtt
     5581 tttagcaaag gtttctgccg cgcgctctcg ccttcgtagg ggcatagcat gctattcccg
     5641 gcgcagatgg ttggctacat gccggccatt ggtctcaaag tacagggata atgtttcgct
     5701 ctcgccttcg tggcggccgc ctttaggcgg gcttagaatt gcctttgctt ttcgtagcaa
     5761 gcccgatcgc ggcttgccgc tttaacgcaa ttctttcgct acgaagcggc cttctttcca
     5821 gccgcccgaa actcccgcgc gcactcatcg catggacaaa gctcccgcag gcaaccgaag
     5881 ctgtaaattc ccgtggcgtg cgcgtcggag aagtcgatct gaatggcata attacccacc
     5941 gccttcgccg ctttagccgt cacgcgcggc ttgtacatgg gcagcaccgc cgcagcggcg
     6001 gccgccttcg gatcggcttc cctcttcgcg cgttcgtcgt tgcatgtcgc gcacgggcag
     6061 ttctcgcgca agtaaggaaa atcgtagcgg ctcgtgtgac cgtccgacca agtgatcgcc
     6121 acgccctcgc ccgtggacac gtagaccttt acgtccaccg gcttctttcg cgtatcaatg
     6181 ggtatcggca ttcaacttat tctagcaaag gcagaagcct gtgccgtcat tctggctgcc
     6241 ctgacgaagg aagggagcgg ggtgtcccgc tttttttctt ttgcgcgaag cgccggaaca
     6301 cgcaaggaag ggctcttggg tcgacggcta atcgagcccg ggagatcaga agaatctctc
     6361 ctggatgctg ccaaggaagc aatgtgtctc ggggcaggct gttctattca taccagaaag
     6421 gaggtttctc gggtgcgtga tccgcgcagg agcgaacatg gtcgatgccg ccccaatcgt
     6481 ggaatagcgt ttccgttcgc tcgttgattt cttcaaggga catcggccga tcatcgcccg
     6541 atcccggaaa cactaggtat tgaatgcctg cccatctccc gccgtggcgc gcatccgacc
     6601 aaattcccag attgtccgcc agcgcgatgg agccttcgcc gagccttcca atgtccgcga
     6661 aaatcgcgta cgaaaacctg ccgtttcgag tgttcaccac aaccgccaaa tctcccaacc
     6721 gcgcgcctga atctcttgcc agttctccgg gcagcgcaat gtacgggatt ttggaggcgt
     6781 ctacgtaccg cgtcggatcg atccgttcct tcgttcgatc tgaaagcgcc gtgcaggaaa
     6841 tgtaatatcc cggaaaagga tcgccgggcc cctgaaccac aggatttcct tcgtcgtccg
     6901 caataatgcc gtcccagtgg cccggttgcc ccgcattgga aaggtcgtcc aatccggtat
     6961 cgtcaggact gtaggcattc ggcgctccgt cagcgtcaat catcaggccg gacgtgaaga
     7021 aaaaagcggc gttgccctgc aatcgccaga tgggcacttg tcgaacagcc tgctcgccgc
     7081 gataaacgtc gaaattcaaa agtagcgatc gcgtagggca gctagtcaat gagtccacag
     7141 cgtcgttcgg cgcggccgcg tgaatattcc ccgccaaacc cgacgcagga agcgccagcg
     7201 ccacaaccaa tggaaaaatt ctcccgatcc gttgcataag gaccagcatt atacctcgcc
     7261 atccgcccta agtccgtggt tggatgccgg tgagatctct tttcgtggtc ccgagatcga
     7321 agaaccatac gacaaggaac acttaggcct gccagaaggg ggcggtgtat aagtgtacgc
     7381 cgcaaggtca aaatcggt
//
LOCUS       DATNDT010000196         3645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1422436, whole genome shotgun sequence.
ACCESSION   DATNDT010000196 DATNDT010000000
VERSION     DATNDT010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1422436"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VN788_06420"
     CDS             complement(<1..750)
                     /locus_tag="VN788_06420"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-54 factor"
                     /protein_id="HXU20197.1"
                     /translation="MLSQRLEPKLWQRQVLTPGLVQMMSVLQMPRLELKEAIAQEIEK
                     NPMLEESLDGGEEVTPEEMHNLLEAERVAEPADASILEATNGSAIEREADAYTSESAG
                     TGDSQSGLSTAAPAETEPVEAKVETDPFDEIDFGEFFNDYLDPGPRTPAAESVDKPSF
                     ETFLSTQVTLSSYLESQLSLVAISDAVRDAADTIIGNLDENGWLTVPIEEIAQIGGHS
                     IEDAGEALTVVQSLDPDGIGARDLRECMLLQI"
     gene            1080..1574
                     /locus_tag="VN788_06425"
     CDS             1080..1574
                     /locus_tag="VN788_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017425108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4145 domain-containing protein"
                     /protein_id="HXU20198.1"
                     /translation="MLRDGGSSQDEFLAYPQTHFRPVPAEVGDPYRKDFAEACAVLPL
                     SGAASAALSRRCLQSILRDKAETTKKNLYDQIEEVIGAGHLPSHIVEGLHAVRNIGNF
                     AVHPMKSTSSGTILDVEPGEAEWNLDVIESLFDFYFVQPALAAKRKAELNRKLKDAGK
                     PEIG"
     gene            1782..2201
                     /locus_tag="VN788_06430"
     CDS             1782..2201
                     /locus_tag="VN788_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20199.1"
                     /translation="MKASIFYRIASVLIVLFAAGHTVGFRRSDPRWGVDALLASMKSL
                     RFNAQGFSRTYWDFFTGFGLFVSVFLILAAVLAWQLGGLPPIALRPLRGLAWVLAICF
                     VGATILSCRYFFMVPIVFSAVISLLLIAAAWLSQKAI"
     gene            complement(2407..3162)
                     /gene="lptB"
                     /locus_tag="VN788_06435"
     CDS             complement(2407..3162)
                     /gene="lptB"
                     /locus_tag="VN788_06435"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXU20200.1"
                     /translation="MQLKLETDDISKTYRGRKVVDDVSLWVKQGEVVGLLGPNGAGKT
                     TSFYMIVGLISPDSGSVLLDGQDVTHMPMYMRARRGISYLPQEPSVFRKLTVEENLMA
                     ILETLPMNGSERRQSMNQRIQQMGLETVRTSKGYTLSGGERRRVEIARSLVIDPKFLL
                     LDEPFSGIDPIQVRELQKIIYDLRNTGIGILITDHNVRETLAVTDRAYIINNGAIFKS
                     GDPDTLGRDPDVRRVYLGEDFDSGRRRRFGTEG"
     gene            complement(3169..>3645)
                     /locus_tag="VN788_06440"
     CDS             complement(3169..>3645)
                     /locus_tag="VN788_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20201.1"
                     /translation="PLFTVVTAPDMVYTDDTRLADYTGGAMLDRPGLRVNSKEIRAFL
                     KPPDDPKENAKSELKGSGDAKPAENQGSSLDHAFADGGVQIVETAKGRTRTGTADHAE
                     YYVSDAKVILRQGKPQLVDTTKGTTRGRELTYFANNDRLLVNGVESQPANSLLLRK"
BASE COUNT          723 a         1071 c         1026 g          825 t
ORIGIN      
        1 gatttgcagc aacatgcatt cgcgcaggtc tctcgccccg atgccatccg gatcaagcga
       61 ctgcaccacc gtcagcgctt cgccggcatc ttcgatcgaa tggccgccga tttgggcaat
      121 ctcctcaatc ggcaccgtca gccaaccgtt ttcgtccaga ttgccgatga tggtatccgc
      181 cgcgtcgcga accgcgtccg atatcgcaac caggctcaac tgcgattcga ggtagctcga
      241 aagcgtgacc tgtgttgaga ggaacgtctc aaatgacggt ttatccaccg attcggcggc
      301 gggagtccgg ggtccgggat ctaggtaatc gttgaaaaac tctccgaaat cgatctcgtc
      361 aaacggatcg gtttcgactt tcgcctctac cggctcggtt tccgctggcg cggcggtgga
      421 tagcccggat tgggagtcac ccgttccggc gctctcgctc gtgtaggcgt cggcctcgcg
      481 ctcgattgcg gagccattcg tcgcttcgag gatcgaagcg tccgccggct cggccacccg
      541 ctcggcttcc agcaggttgt gcatctcctc cggggtcacc tcctcgccgc cgtcgaggga
      601 ttcctcgagc atcgggttct tctcgatctc ctgggcgatg gcttccttca gctccagccg
      661 cggcatctgg agcacgctca tcatctgaac gagccccggc gtcagcacct gacgttgcca
      721 gagcttcggt tctaatcgct gagaaagcat ggctcgactt cataataaca agcggggaca
      781 tggatgaaac cgttaaatgc ggatgggatc gatgttaaaa tccgccggat gggccagtct
      841 tcggcgccga accggcggcg gcgtccgctg tcgaaatccg cgccgggata gacgcgccga
      901 acggcgacat tacccgtcgt tggcggtcag tcaagtgata agctacatct tccatgaaat
      961 gtcctcattg ctccgtggcg ttccatgatt cttggcaaga cgttcgaatt ggcaatcccc
     1021 tcatcgggag agggatagcg cgactgcaaa ggtgcccaga gtgtgacaag gtcgttatta
     1081 tgcttcggga cggtggctct agtcaggatg aatttctcgc ttacccgcaa acacactttc
     1141 ggccagtgcc cgctgaagtt ggcgatccct atcgaaagga tttcgctgag gcgtgtgcag
     1201 ttcttccgct cagcggagca gcgagcgccg ccctgagccg caggtgcttg cagtcgattt
     1261 tgcgagacaa ggccgaaaca acaaagaaaa acctatatga ccaaatcgag gaggtgatcg
     1321 gagcggggca cttgccatct cacattgttg agggcctcca cgccgttcgg aatatcggaa
     1381 atttcgccgt tcatcctatg aaaagcacca gctctggaac aattctcgac gtggagccgg
     1441 gagaagcgga atggaatctg gacgtgatcg aatcgctgtt cgatttctac ttcgtccagc
     1501 ccgccttagc cgcaaagcgg aaagccgaac tgaataggaa attgaaggat gcaggtaaac
     1561 ctgagatcgg gtaacccttc agccttcgat gtcgaagcgg cggcggcgtt tccaagtgga
     1621 tagcccttct cgcggcaaaa gtgaaccact gaacagctcg tggaagctat caattgatgc
     1681 gcttgatgat ccgggtaaag ctgttgccgt ggcgaatgcg aggatttacc atcgaacagt
     1741 agcgagcgtc ttaaccgcca tctcagacta ggggtcgacc aatgaaagcg tccatttttt
     1801 accggatagc ctcagtcctc atcgtgcttt ttgctgccgg ccatacggtg ggattccgca
     1861 ggagcgatcc tcgatggggg gtggatgcgc tactcgcatc catgaaatcg cttcgcttta
     1921 atgcgcaagg cttcagccgg acctactggg acttctttac cggctttgga ttgttcgtgt
     1981 ctgtcttcct catattggca gcggtgttgg catggcaatt gggcggccta cctccaattg
     2041 cgttacgacc gctgcgaggg ctggcctggg tgcttgccat ctgcttcgtg ggggcaacga
     2101 tcctcagttg ccgatacttt tttatggttc ccatcgtgtt ttccgctgtg atctcgctat
     2161 tgttgattgc cgcggcttgg ctctcccaaa aagcgatcta gcgcaataac gggcgcgtcg
     2221 aacagacctc gccgggatgg ccgccggtct gcggacggtc ccgaacgcgc gaaatcctgc
     2281 tctgtctgga gacgaggtaa aactcaatgc taaaagagct gttgccatag cgactcggag
     2341 aatcatcgag cagcagcaag tgtcttaagt gctgtttcag cccacgggcc atcccaacgc
     2401 gatctctcag ccttcggtgc cgaaccggcg gcgccgtccg ctgtcgaaat cctcgccgag
     2461 atagacgcgc cggacatccg ggtcgcggcc cagcgtatcc gggtcacccg atttaaagat
     2521 cgcgccgttg ttgatgatat aggcgcggtc ggtgacggca agcgtctcgc gcacgttgtg
     2581 atcggtgatc agaatgccga tgcccgtgtt tcgcagatca tagataatct tctgcaattc
     2641 gcggacctga atcggatcaa tgccggagaa cggttcatcc agcagcagga atttcgggtc
     2701 aatcaccagc gagcgcgcga tctccacgcg ccgccgctcg ccgcccgaaa gcgtataccc
     2761 tttgctagtc cgtaccgtct ccagccccat ctgctgaatg cgctgattca tgctctggcg
     2821 ccgttccgag ccgttcatcg ggagcgtttc gaggatcgcc atcagattct cttccaccgt
     2881 cagcttccgg aacacagaag gctcctgcgg cagatagctg ataccgcggc gcgcccgcat
     2941 gtacatcggc atgtgcgtga catcctggcc gtccaacagc acgcttccgg aatccgggct
     3001 gatcagccca acgatcatat agaacgatgt cgttttcccg gcgccgttcg gcccgagtaa
     3061 cccgaccact tcgccttgct tcacccacag agaaacgtcg tccacgacct tgcggccgcg
     3121 ataggtcttg gagatgtcgt cggtttcgag cttaagctgc atcgaaaatc acttgcgaag
     3181 aagcaagcta ttggcgggtt ggctctctac gccatttacc agcagcctat cattgttggc
     3241 aaagtaggtc aattcgcggc cccgcgtcgt gccttttgtg gtgtccacta actgcggctt
     3301 tccctggcga agaattactt tcgcatcgct gacataatat tccgcgtgat ccgccgtgcc
     3361 cgtccgcgtg cgtcccttgg cggtctcgac gatctgcaca ccgccgtcgg caaaggcgtg
     3421 atcgagagat gagccttgat tctccgccgg tttcgcatcg ccggaacctt tcagttcgct
     3481 tttggcgttt tctttgggat cgtccggagg cttcaaaaac gcgcgaatct ccttcgagtt
     3541 cacgcgcagt cccggccggt cgagcatcgc gcctcccgta taatcggcca gacgcgtgtc
     3601 atcggtatac accatatccg gcgccgtcac tacggtaaat aacgg
//
LOCUS       DATNDT010000197         4256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_215505, whole genome shotgun sequence.
ACCESSION   DATNDT010000197 DATNDT010000000
VERSION     DATNDT010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215505"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..520
                     /locus_tag="VN788_06445"
     CDS             <1..520
                     /locus_tag="VN788_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HXU20202.1"
                     /translation="VVIFPAKFDGLYARLDRPHSEIAPWSIWISYSPDLRFWGESQLI
                     IKPEQYHWDEMKVGPGAPPIKTDQGWLSIYHGVFQTMDGSVYRLGVALHELQNPAKIV
                     GVGDSWILQPEDPWEITGYVHNVVFTCGAVPESDGTIKIYWGGADTVMCVGEANISDL
                     VALCLERGRPAK"
     gene            complement(863..1246)
                     /locus_tag="VN788_06450"
     CDS             complement(863..1246)
                     /locus_tag="VN788_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXU20203.1"
                     /translation="MALTRHYEETVRVRVQRDAGFREALLREGIENFLSGDVETGKII
                     LRDSINATVGFPKLSEITHRSAKSLMKMLGQRGNPQARNLFEIVKYLQETEGVQFEVW
                     PKRVGSRTKRNELLAAPRGARPSNF"
     gene            complement(1322..1681)
                     /locus_tag="VN788_06455"
     CDS             complement(1322..1681)
                     /locus_tag="VN788_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013189767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HXU20204.1"
                     /translation="MSVNVNEVIRKLNPADRKKVEDRAAEIIAEEMSLRDLRKARKLT
                     QARVAKTLGITQDSVSRLEKRSDLLISTLRKTVKAMGGDVRIVAEFPDRAPVVLAELS
                     EDDPPRKSPRKHSHAEV"
     gene            complement(1684..2043)
                     /locus_tag="VN788_06460"
     CDS             complement(1684..2043)
                     /locus_tag="VN788_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXU20205.1"
                     /translation="MKWAVEMADEFEPEFQALHEDVRTEILALSIVLEQFGPQLGRPR
                     VDTLKGSQHANMKELRFSAADGEWRVAFAFDRRRAAILLVAGDKSGGSQKRFYRELIR
                     KADERFDAHLARIGKGK"
     gene            2196..2582
                     /locus_tag="VN788_06465"
     CDS             2196..2582
                     /locus_tag="VN788_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20206.1"
                     /translation="MPVQSPENLAHRRQSALWLFYGAAVVSAIAISAVAKVPFLRIPF
                     LALAVLTAGAAILFLLRFLRSNDEREQQINYRALTFAFAGTLIFSLAIGYSQAVGFHP
                     VSWLGIPALMVVLWSIGLVLYSWRYR"
     gene            2579..2797
                     /locus_tag="VN788_06470"
     CDS             2579..2797
                     /locus_tag="VN788_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU20207.1"
                     /translation="MKTRLRVLRAEVEWSQAELAARVGVARGTINAIETGKYDPSLPL
                     AFKIARAFGKNVEEVFLYAEDQDHASRT"
     gene            3068..3742
                     /locus_tag="VN788_06475"
     CDS             3068..3742
                     /locus_tag="VN788_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20208.1"
                     /translation="MGKFRVVPTEDLARFGYQGDQAHMEADIRNLRKHGLIDQRTIEG
                     HSSYSTKVLTLTKEGQRLLKHAQLVSNRQVTYHGFAKPKEARHDAGLYRLYQKVAKEI
                     ERSGGNVRRVILDLELKKELYQAISRMRPDKDPAYERIAIASRFDLKFVNDKIPIPDL
                     RIEYEDECRDVHRLDLEVATRDYRPQGLGEKAKAGFHVFARQQDHDRLRRVLDSQEIT
                     ARIFVL"
     gene            3739..>4256
                     /locus_tag="VN788_06480"
     CDS             3739..>4256
                     /locus_tag="VN788_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20209.1"
                     /translation="MKFLPIHIDALRGFGYTEEEARFLYLVATHSGYFTCQQYLHFIQ
                     TKPGKRSVAFAQKVIGKKHAKTKSYLRHGRVFHLFSRNLYEAIGRENVRFRRVHSTQY
                     IRMRLIALDFILRNQDVTYLETEGQKLTFFCDQMGIAKKILPHKRYSGAIKGQFTDRY
                     FVDKFPMFYNPS"
BASE COUNT          951 a         1187 c         1143 g          975 t
ORIGIN      
        1 cgtggttatt tttccagcga agtttgacgg gctctatgcc cggcttgatc gtcctcattc
       61 ggaaatcgct ccctggtcca tctggatttc ctactctcca gatctgcgtt tttgggggga
      121 gtcccagctc atcataaagc ctgaacaata ccactgggac gaaatgaagg tcggtcctgg
      181 tgcgccgccg atcaaaaccg accagggatg gctctccatc tatcatggtg ttttccagac
      241 aatggatgga tctgtctatc gtttgggggt ggcgcttcat gagctacaga atcccgcgaa
      301 gattgttggt gtgggcgact catggatttt gcaacctgag gacccttggg aaatcacggg
      361 atacgtgcac aatgtggtct ttacgtgcgg cgcggttccg gagagcgatg gaacgatcaa
      421 gatctattgg ggtggcgcag atacggtcat gtgcgttggt gaagcaaaca tctcggacct
      481 ggttgcacta tgcctggagc gcggaagacc ggccaagtag ctaagtttgt aagcggtgtc
      541 gatgcggcgt ttgcagtaga gcgcggccac aacaacatga gctgtgaaga gaaaaaacgc
      601 cttgccagcg aatacgaaaa cgccacgacg aagttttcgc acggggtcgc cgagctccgc
      661 aaaagaatgg gcacctcgac taaagaggaa tatcaacgac ttgaccgggc gtctaatgaa
      721 gcgagcgtca tttccgaaca cgctcggctt gcgcttgagc agcacgcgcc gctcatcgtt
      781 gctgataatt ccgacaagca aattccattc aaccatcgcc gagggatatt ggtcgaggat
      841 gcgggtttca cgcaactgcg ggctagaagt tcgaaggtcg tgccccacga ggtgcggcca
      901 acagttcgtt gcgtttcgtg cgcgagccga cacgcttagg ccaaacttca aactgcacgc
      961 cctcggtttc ctgcaagtac ttgacgatct caaacagatt ccgcgcttgg ggattgccgc
     1021 gctgcccaag catcttcatc agactctttg cagaacgatg cgtgatttcg ctcaatttgg
     1081 gaaaccccac tgttgcgttg atggaatcgc gaaggatgat cttccctgtt tcgacatctc
     1141 cggaaagaaa attctcaatc ccctcgcgca agagcgcctc gcgaaaaccg gcatcgcgtt
     1201 gaacgcgtac ccggacggtt tcttcataat gccgtgtcaa cgccattgct ctctccgagg
     1261 acaagcgcat tgaccgcctg ggcggcccca acttcttgac atcactgaga accggggagc
     1321 cttaaacttc agcgtgcgag tgtttccgag gtgatttgcg cggtgggtcg tcctcggata
     1381 gttccgcaag cacgactggc gcgcgatcag ggaattcagc gacaattcgc acgtcgccgc
     1441 ccatcgcttt gaccgtcttt cgcagtgtag agattaacag gtcactgcgc ttttcgaggc
     1501 gtgaaacact gtcttgagtg attccgaggg tcttggcgac acgcgcctgc gtgagcttgc
     1561 gggcctttcg gagatcacgc aagctcattt cctctgcgat tatctcagcc gcgcggtcct
     1621 cgaccttctt gcggtcggct ggattcagct tccgaattac ctcattcacg tttacggaca
     1681 tggttacttc ccctttccga tccgagcaag atgcgcgtca aagcgctcat ctgccttcct
     1741 tatcagttcg cggtagaaac gcttttgact cccacccgat ttgtctcccg ccaccagtaa
     1801 aatcgccgca cgcctgcgat caaaggcgaa ggccactcgc cattcaccat ctgccgcgct
     1861 gaatcgcaat tccttcatgt tcgcgtgttg ggagcctttc agtgtgtcaa cccgtggccg
     1921 acccaactgc ggcccaaact gttctaaaac aatcgaaagc gccaatatct cagtccgcac
     1981 atcctcgtgc agtgcctgaa attccggttc gaattcatcg gccatctcaa cggcccattt
     2041 cacgcgtaca aatatggcat ggaatacatt atggtgtcaa ctccatatat ttgcgggtga
     2101 gagctgcgaa agggcggtgt acgggaccgc aagaaccttg tgcttgacac tatgtacgct
     2161 atacattaca ctgcacgaag ttttcgggag ggctcatgcc tgttcaatcc ccggagaatc
     2221 tagcgcaccg ccgtcaaagt gcgctctggc tcttttatgg cgcagcagtg gtttccgcaa
     2281 tcgcaatcag cgcagtcgcc aaggttccct tcctccgcat tcctttcctc gccctcgctg
     2341 tcctgacagc aggggccgcg attctgttcc tgcttcgctt tctgcgctcc aacgatgagc
     2401 gcgagcaaca aatcaactac cgcgccctga cgtttgcctt cgcgggcaca ctaattttct
     2461 cgctcgctat tggttattcg caggctgtcg gctttcatcc cgtctcatgg ttgggaatcc
     2521 cggcattgat ggtcgtgctg tggagcatcg gcctcgtttt gtattcatgg cggtaccgat
     2581 gaagacccgg ctacgtgttt tacgtgcaga agtggaatgg tcacaggccg agctcgcggc
     2641 gcgtgtcggg gtagctcgcg ggaccatcaa tgcgattgaa actggcaagt acgatccgag
     2701 cttgccgctg gcattcaaga tcgcccgcgc gttcggaaag aatgtcgaag aggtgtttct
     2761 ctacgccgag gatcaggatc acgcgtcccg tacctgagtc cgaaggctaa gtgaaaaggt
     2821 tcctatggac cggccttaca gcatctccga tttcgctttg ccgtcccgtt gggattcgga
     2881 ctcacatccc agtgtaatcg aacgggcggg caggtcccga agcgacgaaa gaacacgtat
     2941 tcagacagac cgcgaaaaag gttccaattc tggcgcgtcg cgaggctcgg atcggtccag
     3001 aacaatctac cgtgggcgcg accgtgagta ttctctccgt gattcggaag tccgtacgtt
     3061 aaccgatgtg ggcaagtttc gcgtcgtgcc gaccgaggac ttggcacgat tcggctacca
     3121 gggcgatcag gctcacatgg aagcggacat cagaaatctg cgaaagcatg gcctaatcga
     3181 ccagcggaca attgaaggtc attcctcgta ttcgacgaag gtgctgacgc taacgaaaga
     3241 gggtcaacgg ctgctcaaac atgcgcagct tgtgtccaac cgccaggtga cgtatcacgg
     3301 ctttgcgaag ccgaaagagg cccgccacga cgccggactc tatcgactct atcagaaggt
     3361 cgccaaagag atcgaacgtt cgggaggcaa cgtccgccgt gtgattctcg atttggagct
     3421 gaagaaggag ctctaccaag ccatctcaag aatgcggcct gataaggacc cagcctatga
     3481 gcgcattgcc atcgccagcc ggtttgattt gaagtttgtg aatgacaaga ttccgattcc
     3541 cgacctacga atcgagtatg aggatgagtg ccgagacgtg caccggctcg atttagaagt
     3601 agccactcgc gactaccgac ctcagggtct gggcgaaaaa gccaaagctg gattccacgt
     3661 ttttgctcgg cagcaggacc acgacagact acgtcgcgta ctcgattcac aggaaatcac
     3721 ggcaaggatt tttgtgctat gaaatttctt ccgatccaca tcgacgccct gcgcggcttt
     3781 ggctataccg aggaagaagc gcgtttcctc taccttgtgg ctacgcattc cgggtacttc
     3841 acctgtcagc agtacttgca tttcatccag acgaagccgg gcaagcgcag cgtggcattt
     3901 gcgcaaaagg tgatcgggaa aaaacatgcg aagacgaagt cgtatctccg gcatggccgc
     3961 gtgtttcacc ttttctcacg caatctgtat gaagccatcg gcagggaaaa cgttcgcttc
     4021 cggcgcgtcc attcaaccca atacatccga atgcggctca ttgccctgga tttcattctc
     4081 cgaaatcagg acgtaacgta cctcgaaact gaagggcaga agctcacatt tttctgcgac
     4141 caaatgggaa tcgccaagaa aatccttccc cacaagcggt attcgggggc gatcaaaggg
     4201 cagttcaccg accgctattt cgtggacaaa ttcccgatgt tctacaaccc cagcat
//
LOCUS       DATNDT010000198         3168 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2222, whole genome shotgun sequence.
ACCESSION   DATNDT010000198 DATNDT010000000
VERSION     DATNDT010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3168
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2222"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VN788_06485"
     CDS             <1..742
                     /locus_tag="VN788_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXU20210.1"
                     /translation="IRKIYIVSEDGEQKEYSVPRGVHINVQEGERVRSGDPLMDGPRN
                     PHDILHVLGEKELQKYLVNEIQEVYRLQGVNINDKHLEVISRQMMRWVKIEDIGDTEF
                     LPEEVVDKFRFREENARVIEAGQRPAAGKPVLLGITKASLSTDSFISAASFQETTRVL
                     TEAAINGKVDYLRGLKENVIMGRLIPAGTGMEYYRAVKIAGEDVVEEEVVTEPDVLSE
                     SIPGYDEDARSLYVGGLSEDPGEETLAE"
     gene            complement(791..1594)
                     /locus_tag="VN788_06490"
     CDS             complement(791..1594)
                     /locus_tag="VN788_06490"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20211.1"
                     /translation="MKEKLVQAFGGLADSTIAAAPKVAVGILLIVAALIVAKIIESVL
                     RYALTKVRFDSLLGRVGVDRALQRLGIRQELAILIPRLVYFLVLLLLARTAGDALGLV
                     AISNGIGAFFSYLPNIVAALVLLILGSTVGQFAGQTVSQSAESSGIEFAPALGKLVSG
                     IILFVCAMMAIAQLKIDTEIIRIVTGLVLGGAALAFGLSFGLGTRDIVRNIAAGFYVR
                     KILVIGRPLEIAGQRGLLRAITATHVILQSGDSETTVSNATILDQVAKQ"
     gene            complement(1591..1848)
                     /locus_tag="VN788_06495"
     CDS             complement(1591..1848)
                     /locus_tag="VN788_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20212.1"
                     /translation="MTPGGSDSNGPPGAHREQPIRELMEQGREPSPDFMQRIQRKIYR
                     RAAGSQLASYSWHLPKIILVEMASLLSHLFKTFGTDKESEP"
     gene            complement(1845..2453)
                     /locus_tag="VN788_06500"
     CDS             complement(1845..2453)
                     /locus_tag="VN788_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU20213.1"
                     /translation="MKPNEESKDLDEELLRAARNAPEGDLRAFEELVQHHQKRILANC
                     RYLTRDESIAEDLAQEVFVKAFFGLRAFEGRSSFRHWLQTIKVHHCLNHLKKREGKAT
                     LAMDEEAVERYEQLHVQPVAAEELEAVERRKRIDGILNAMPATLRIPLIMRDMDELSY
                     EEVAASLGIGLSAVKMRIKRAREQFRRRYRGEEAALGAQTSR"
     gene            2695..3018
                     /locus_tag="VN788_06505"
     CDS             2695..3018
                     /locus_tag="VN788_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20214.1"
                     /translation="MKLNQLILLILAAFSLAVARGRLPRRFPIARKNGGKRPRGNTGR
                     YQYRNPSGTGRTARQRQGIGEKIVKNRPYRAKNELVEKHILPAAVYAKSRTRSSRSKS
                     KHGVN"
     gene            3053..>3168
                     /locus_tag="VN788_06510"
     CDS             3053..>3168
                     /locus_tag="VN788_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HXU20215.1"
                     /translation="MLKDFKAFIMKGNVLDLAVAVIIGVAFGAVVTSAVNDVV"
BASE COUNT          741 a          920 c          886 g          621 t
ORIGIN      
        1 catccgcaag atttacatcg tcagcgagga cggggagcag aaagaatact ccgttccgcg
       61 cggcgttcac atcaatgtgc aggagggaga gcgtgtccgt tccggcgatc cgctgatgga
      121 cggtccgcgc aatccgcacg atattctgca cgtgctcggc gaaaaagaac tgcagaaata
      181 cctggtgaac gaaatccagg aagtctatcg tctgcagggc gtcaacatca acgataagca
      241 cctggaagtc atctcccgcc agatgatgcg ctgggtgaag atcgaagaca ttggcgacac
      301 cgagttcctc cccgaagagg tcgtggacaa attcaggttc cgcgaagaga acgccagggt
      361 catcgaagcg ggccagcgcc cggcggccgg gaaaccggtg ctgctcggga tcacgaaggc
      421 atcgctttcc accgattcgt ttatttccgc cgcgtcgttc caggagacca cgcgcgtgct
      481 caccgaagcc gccatcaacg gcaaggtgga ctacctgcgc ggcctgaagg agaacgtcat
      541 catgggccgc ctgattccgg ccggcaccgg catggagtat taccgcgccg tcaagatcgc
      601 cggcgaggat gtggtcgagg aagaggtcgt cacggagccc gatgttctct ccgagtccat
      661 tcccggctac gacgaggatg cccgcagcct ttacgtcggc ggcctttccg aagatcccgg
      721 cgaagagacg ctggcggaat aagccactct ttccattcag cgcttcgagc ggccgcttaa
      781 aatgcggccg ctactgttta gcgacctgat ccagaatggt tgcgtttgaa acggtggtct
      841 cgctgtcgcc ggattgcagg atcacgtggg tcgcggtaat ggcccgcaga aggccccgct
      901 ggccggcgat ctcgagcggc ctcccaatca cgagaatttt gcgcacatag aagccagccg
      961 cgatattccg aacgatgtcg cgggtaccca gtccgaacga gagcccgaag gccagtgcgg
     1021 cgccgcccaa caccaggccg gtgacgattc gaatgatctc ggtatcgatc ttcagttgcg
     1081 cgatggccat catggcgcaa acgaagagga tgattccgga gaccagtttc ccaagtgccg
     1141 gagcgaattc gattcccgaa ctttccgccg attgcgaaac agtctgaccc gcgaattggc
     1201 caaccgtgct gcccagaatc aaaagcacga gcgccgcgac gatgttgggc aggtaggaga
     1261 aaaacgcccc gatgccgttc gagattgcga ccaagccaag ggcatctccc gcggtcctgg
     1321 cgaggagcag caaaaccaga aaatacacga ggcgcgggat gaggatggcc aactcctggc
     1381 gtatgccgag ccgctgtagc gcccggtcga cgccgaccct gcccaagagg ctatcgaaac
     1441 gcactttcgt cagcgcatat cgcaaaacgc tttcgattat ttttgcgacg atcagcgccg
     1501 cgacgataag cagaatcccg accgcgacct tcggggccgc cgcgatcgtg gagtcagcca
     1561 ggccgccaaa agcctggacg agcttttcct tcatggttca gactccttgt ccgttccaaa
     1621 cgttttgaag agatgactaa gcagacttgc catttcaacc aggattatct ttggcaaatg
     1681 ccaggaatag gaagctaact gcgagccggc cgcccggcgg tagatctttc tctgaatccg
     1741 ctgcatgaaa tcgggagacg gctcgcggcc ttgttccatc aattcccgga tgggctgttc
     1801 ccgatgcgcg ccgggcggtc cgttcgaatc gcttccaccc ggagtcatct tgatgtttgc
     1861 gctcctagcg cggcctcttc gccccgatag cgacgtctga attgttcgcg cgcgcgcttg
     1921 attctcatct tgaccgccga aaggccaata ccgagcgaag ccgcgacttc ttcgtaagaa
     1981 agctcgtcca tgtcgcgcat gatgagcggt atccggagtg tcgcgggcat cgcgttcaaa
     2041 atgccgtcga tacgcttacg gcgctccacc gcttccagct cctcggccgc aacaggctga
     2101 acgtgcaact gctcataccg ttccaccgcc tcttcatcca ttgccagcgt tgccttgcct
     2161 tcgcgcttct tcagatggtt caagcagtga tgcactttga tcgtttgcag ccagtgacgg
     2221 aatgacgagc ggccttcaaa agcgcgcaat ccgaagaaag ctttgacaaa cacctcctgg
     2281 gcaagatctt cggcaatgct ctcgtcgcgg gtcagatacc ggcagttggc cagaatccgc
     2341 ttctgatggt gctgcactaa ctcctcgaac gcgcgcagat caccctccgg ggcattgcgg
     2401 gccgcccgca gaagctcctc atcgagatcc ttagactctt cgttcggctt catggctcac
     2461 tgttcagaat tccgaggatc tcataactct ggacagttcg catccgcgca aagtcatatt
     2521 gaagccaact gtactaggct tactcaaccg tgataccaga gttttggatt gccagatgtg
     2581 actcggccaa acaaataact gtctcaacta atgaaagggc gctgaaccct tcgggaaaat
     2641 taaaaggcac cgtgggcgcc caaagtcaaa agaactggca gaggaaattc cgatatgaaa
     2701 ctgaatcaac tgatcctgct gatcctagcg gcgttctcat tggccgtggc tcgcgggcgg
     2761 ctaccaaggc gcttcccaat cgcacgtaaa aacggcggca aacgtcctcg cgggaacact
     2821 ggtcgatatc aataccgcaa ccccagcgga actggaagga ctgcccggca gcggcaaggc
     2881 ataggcgaaa agatcgtcaa gaatcgtccg taccgggcaa agaatgaact cgtcgagaag
     2941 cacattcttc cggcggccgt gtacgccaaa tcaagaacaa gatcgtcgcg aagcaaaagt
     3001 aaacatggcg tgaattaacc tttaaaacac aatgggagaa ggaaagaaaa acatgctgaa
     3061 ggatttcaaa gcctttataa tgaaaggcaa cgtactcgac ctcgccgtcg ctgtaattat
     3121 tggcgtggca tttggggccg ttgtaacctc tgcggtgaac gatgtcgt
//
LOCUS       DATNDT010000199         4102 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_294074, whole genome shotgun sequence.
ACCESSION   DATNDT010000199 DATNDT010000000
VERSION     DATNDT010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4102
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_294074"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /gene="wecB"
                     /locus_tag="VN788_06515"
     CDS             <1..1054
                     /gene="wecB"
                     /locus_tag="VN788_06515"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HXU20216.1"
                     /translation="DVHLGVGSGSHAVQTAEVMKRFEEVLLAERPDLLIVVGDVNSTI
                     GCALVASKISFDAGGSRPLIAHVEAGLRSFDRGMPEEINRILTDHLADLLFVTEQSGL
                     DNLREEGISDHKVHFVGNTMIDSLFTFKERADRSDILDRLNIRRPNGASAEVQRYALL
                     TLHRPSNVDQCATFLNILEGLDSLSSACPVIFPVHPRTRKRISEFGLERFFRSESANG
                     NGRNATSGDGIQLVEPLGYLDFLCLMAHAAVVVTDSGGIQEETTCLGVPCVTVRDNTE
                     RPVTVTVGTNVLAGTSKEGIRTATHKQLFSRVSGSVPEAWDGKTSQRILEVVCREFAK
                     KTLPTSPEMATCTRTS"
     gene            1079..1942
                     /locus_tag="VN788_06520"
     CDS             1079..1942
                     /locus_tag="VN788_06520"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006893119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU20217.1"
                     /translation="MPRYVIITPVRDEEAHIASTIASVRAQTVLPSEWVIVDDGSTDH
                     TGEILDEAAAQCSWMRVIHRQNRGFRKSGGGVMEAFYDGYNSLQWHDWDFIVKLDADL
                     SFSADYFEECFKHFVRDPKLGIGGGDIQHEINGKLKVEENPRFHVRGATKIYRNACWE
                     DIGGLWPAPGWDTIDEVKANMLGWKTSSFPELPLVHHKLTGSADGWARDRVKHGLVCY
                     ICGYHPLFVVASCVRRIGQKPYFLAAAAVLYGFLKGHITHAPRLNDRSYVSYIRDQQL
                     RRLCGMQTIWR"
     gene            1966..3867
                     /gene="asnB"
                     /locus_tag="VN788_06525"
     CDS             1966..3867
                     /gene="asnB"
                     /locus_tag="VN788_06525"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXU20218.1"
                     /translation="MCGICGKLMFSSDAKVPHALVNAMADTIRHRGPDDEGYYVSGSV
                     GLGFRRLAIIDLQTGHQPLSNEDGTVWIIFNGEIYNYHELHSVLVAKGHVFKTRTDTE
                     VIVHLYEEFGPECLQKLRGMFAFAVWDEKAKSLFVARDRVGIKPLYYHISEESLIFAS
                     EIKAILADPSVARNISPDVIDRFLTFLYLPGEQTLLAGIQKLEPGHYLIAKNGRATIV
                     QYWDLCFSDENSNDNLAEAERALEDLLAETVKLHMIADVPVGVLLSGGVDSTGVLSFA
                     VNGTTKKVSSFTVGFADTHVADERPYAKIAADKFGTQHHDMTIAASDFASFMPQYVWH
                     MEEPVCEPPAIALYYVSKLAREHVTVLLSGEGGDEAFAGYSNYRNLVWLERIKRGLPL
                     KAAAAKSLKLADSLFHAHRLGRYAPLMNDRFPSYYYSRTSNPYRFAGNSLGDVYASDF
                     AQAIDRERTLEPVRKLQNHVRGQNTLDAMLYIDTKTWLPDDLLIKADKMTMANSIELR
                     VPLLDHKILEFAASLPPRFKLNGLNLKYILKRALSQKIPQAIRDRKKTGFPVPYERWL
                     RADLKDLVYSVLTDSRTVERGYFRKEGIEGLLKLNGNGHDYSKEIFSLLSLELWQRTF
                     IDGEKVVLQ"
     gene            3854..>4102
                     /locus_tag="VN788_06530"
     CDS             3854..>4102
                     /locus_tag="VN788_06530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20219.1"
                     /translation="MSSSNFRRAAFSLGAKTFWRIPGAFNIARVVGPDYWLRCVVFHH
                     ISAKDLPFTHGINVRTSPEEFEMALAFIAANYTPVRLDD"
BASE COUNT          942 a         1054 c         1126 g          980 t
ORIGIN      
        1 cgatgttcat cttggagtgg gatccggttc gcatgcggtt cagaccgcag aggtgatgaa
       61 gcgattcgaa gaagtcttgc ttgccgagcg cccggacctg ctcattgtcg tgggtgatgt
      121 taactcaacg attggatgcg ccctcgttgc atcaaagatc tctttcgatg cagggggcag
      181 ccgcccgctt attgcgcacg tcgaagccgg actacgttcc ttcgaccgag gcatgccgga
      241 agaaatcaac cgcattctta ccgatcactt ggccgaccta ttgttcgtca cagagcagag
      301 cggcctcgat aatttgcgcg aggagggcat ttcagatcat aaggtgcatt tcgtcggcaa
      361 tacgatgatc gattcgctct tcacgtttaa agagcgagcg gaccgctccg atatccttga
      421 tcgattgaac attcgccgac caaatggagc gagtgctgag gttcagcgct atgccttgct
      481 tacgctgcac cgtccatcaa atgtggacca gtgcgcgacc tttctgaata tcctcgaggg
      541 gcttgactca ctttcgagcg cgtgccctgt tattttcccc gtgcatccga ggacaaggaa
      601 acggatatct gagtttgggc ttgagcgatt tttccgctct gaaagtgcga acggcaatgg
      661 ccgcaacgca actagcggcg atggcatcca gttggttgag ccgctggggt atctggactt
      721 tctttgtttg atggcgcacg cggccgtggt tgtgaccgac tcaggaggca ttcaggaaga
      781 gaccacctgt ctcggagttc cctgtgtcac ggttcgagac aacacggaac gccccgtcac
      841 ggtaaccgtc ggtacgaacg ttctagctgg tacgagcaag gaaggaattc gaacggccac
      901 gcacaaacaa cttttttcca gagtatctgg ttcggtgccc gaggcttggg atggaaagac
      961 ctcccaacga atcctcgagg tagtttgtcg cgagtttgcc aaaaaaactt tgccaacttc
     1021 gccggagatg gcgacgtgta cacgaaccag ctaaggcttc gctcttaggt cgtcaccgat
     1081 gcctagatac gtgatcatca ctcccgtccg cgatgaagaa gcgcacatcg cgtccacaat
     1141 cgcttccgtc cgtgcccaga cggtgcttcc ttctgagtgg gtgatcgtcg atgacggatc
     1201 gacggaccat acaggtgaga tcctggatga agcggcagcg caatgctcgt ggatgcgcgt
     1261 aatccaccga cagaataggg gctttcgcaa gtcggggggt ggcgtaatgg aggcgttcta
     1321 tgatggctac aacagcctcc agtggcacga ttgggacttt attgtaaagc tcgatgccga
     1381 tcttagcttt tccgccgatt atttcgaaga atgcttcaag catttcgttc gcgatccgaa
     1441 gctcggcata ggcggcggcg atattcaaca cgaaatcaac ggcaagctca aagtcgaaga
     1501 gaatccgcgg ttccacgttc gcggcgcgac caaaatctat aggaacgcct gctgggaaga
     1561 catcggtggc ctgtggccag cgcctggttg ggacacaatt gatgaggtca aggcgaatat
     1621 gttggggtgg aagacctcct cgttcccaga attgccgctg gttcaccata aattgaccgg
     1681 gtccgccgac ggctgggcgc gcgaccgagt gaagcacggt ttagtttgtt acatttgcgg
     1741 ctatcacccg ctgtttgttg tggccagttg tgtgcggcgt atcggccaga agccttattt
     1801 tcttgcggca gcagcggtgc tgtacgggtt cctcaagggg catatcactc acgcgccgcg
     1861 tttgaatgat cgctcttacg tttcgtacat acgtgatcag caacttcgcc gtctttgcgg
     1921 tatgcagacg atttggcgtt aggtctccgg agccatcgaa ctcacatgtg tggtatttgc
     1981 ggaaaattga tgttttcaag cgatgcgaag gtgccgcatg cgctcgtgaa tgcgatggca
     2041 gatacgattc gtcaccgcgg cccggatgat gaagggtatt acgtgtcagg ctcggtcggg
     2101 ctaggttttc ggagacttgc aatcatagat cttcaaaccg gccatcagcc gctctcaaac
     2161 gaggacggaa cggtctggat catcttcaac ggggagatct ataattatca cgagctgcat
     2221 agcgtccttg tagccaaagg ccacgtattt aagaccagaa cggatactga agtcatcgtt
     2281 cacttgtacg aagagtttgg ccccgaatgc cttcagaagc tgcggggcat gttcgcattt
     2341 gccgtctggg atgaaaaagc gaagagcctt ttcgtcgcga gagatcgagt cggaataaaa
     2401 cccctttact atcacatatc agaagaatcg ctcatttttg cgtcagaaat taaagcgatc
     2461 cttgccgatc cttctgttgc tcgaaatatt tctcccgatg ttatcgatag atttttgact
     2521 tttctctatt tgcctggaga gcagactcta ctagcgggta tccagaaact cgagccgggg
     2581 cactacttaa ttgcaaagaa cggtcgagca acgatcgtac aatattggga cctgtgcttc
     2641 agcgatgaga attccaacga taacttggcc gaagcagagc gtgcgttaga agatcttctg
     2701 gccgaaaccg tgaagctcca catgatcgca gacgtccccg taggcgtcct gctaagcggt
     2761 ggggttgact caaccggcgt gcttagcttt gccgtaaacg gaacgactaa aaaggtcagc
     2821 agtttcaccg tgggattcgc cgacactcat gtagcggacg agcggccgta cgcaaaaata
     2881 gccgccgata aattcggtac gcagcaccac gacatgacga tcgcagcgag cgatttcgca
     2941 tcgttcatgc cgcaatatgt gtggcacatg gaagagcccg tatgcgaacc gcccgcaatc
     3001 gctctgtact acgtctcgaa attggctagg gaacatgtga ctgtgctgct ctcgggcgaa
     3061 ggcggtgatg aggcgtttgc gggttacagc aattatcgca acttagtttg gcttgaacgc
     3121 atcaagagag gcttgcccct aaaggcagcg gctgcgaaga gtcttaagct agccgattcg
     3181 ctgtttcatg cgcaccggct cgggagatac gcgccattga tgaacgaccg cttcccgagc
     3241 tattactaca gccgcacctc gaatccttac cgtttcgcgg gcaattcgct gggcgacgtc
     3301 tatgcgtctg attttgctca agcgatcgat cgcgagcgca cgcttgaacc ggttcgcaag
     3361 cttcaaaacc atgttcgggg ccaaaacaca cttgatgcaa tgctctatat agacacgaag
     3421 acatggctac ctgacgacct actgatcaaa gcggacaaga tgacgatggc gaactcgatc
     3481 gaacttcgtg tcccactgct cgatcataaa atccttgaat tcgctgcatc gctgccgccg
     3541 cgtttcaagt tgaatggatt aaatctaaaa tatattttga agagggctct gtcgcaaaaa
     3601 atccctcaag ccatccgcga tcgcaagaag actggttttc ctgtccctta cgaaagatgg
     3661 ctccgcgcag atctaaagga cctcgtttac tcggttctca ctgacagccg aaccgtagaa
     3721 cgcggctact tccggaaaga gggcattgaa ggcctgctca agctgaatgg caatgggcat
     3781 gactactcga aggagatttt ttcgcttttg agcttggagc tgtggcagcg cacatttatt
     3841 gatggagaaa aagttgtcct ccagtaattt tcgccgagcg gcattttctc ttggcgcgaa
     3901 aacattttgg cggatccccg gcgctttcaa catcgcgcgc gtggttggac ccgattactg
     3961 gctgcggtgc gttgtgtttc atcatatctc agcgaaggat ttgccgttca cgcacgggat
     4021 taatgtccgc acctccccag aagaatttga aatggcgctc gcgttcatcg ccgcgaatta
     4081 cacgccggtc cgtctggatg ac
//
LOCUS       DATNDT010000200        10136 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1792824, whole genome shotgun sequence.
ACCESSION   DATNDT010000200 DATNDT010000000
VERSION     DATNDT010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10136)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10136)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10136
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1792824"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VN788_06535"
     CDS             <1..190
                     /locus_tag="VN788_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXU20220.1"
                     /translation="TRIWRVAEELEYGIVGINTGLISTEVAPFGGVKQSGLGREGSKY
                     GIEEFLEIKYLCLGGMNF"
     gene            complement(376..1500)
                     /locus_tag="VN788_06540"
     CDS             complement(376..1500)
                     /locus_tag="VN788_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20221.1"
                     /translation="MSTKKISRREWIASSTAALGAAAFAEPGDAGSRAQAPLQSVGGR
                     EQSSPPQPMTAPDGQQLYQGTRRADWMQNPRYAWGVMTHYLADWQSQVNKLDMNVDRW
                     NKMIDAWDVEGMSKRLASVGAGHYQLSIGQNSGYYLSPNATYDKTTGIAPSKCSRRDL
                     VADFYEPLRQRDIKLMVYLPSGAPNGDKVAKDALEWQSGGYPNKNFQRKWEAIIREWS
                     QRWGKRIAGWWLDGVVYPDSMYRSPEPPNFTSLAAAARVGNQDACVAFNPGVYNRLFA
                     MSPDEDFTAGEIDDPNLVKAMHQANGRIDGSQIHMLCFLGEHWGRGEPRFSTEQAIAA
                     TKKVRDWGGAVTWDVPVAMDGAISNAFMDQLEAIGKAFPR"
     gene            1904..2026
                     /locus_tag="VN788_06545"
     CDS             1904..2026
                     /locus_tag="VN788_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20222.1"
                     /translation="MKIQMCRQQKNKPPRRVGTDVTAYYARKSFDAVKAQQAAL"
     gene            complement(2038..5757)
                     /locus_tag="VN788_06550"
     CDS             complement(2038..5757)
                     /locus_tag="VN788_06550"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU20223.1"
                     /translation="MRLSVGLWKALALFLLLLVIAAGGIHAQTTSGEISGQVVDPTGA
                     AVVGATVTLTNQLTGDTRAAKTQDSGQFAFVDVQPGQFAVSVEATGFKKLEKSGLSLS
                     ASQRLSAGTLKLELGAVSQSVSVTATPTPVQTDSAERSALLDSNELEHISTPGHDVLA
                     LVRLLPGVVKDGEGASQLGTESAGAVSGTREVSNSVSVDGVNGNPRGGGNRFDTPLNS
                     EAVGEVKVLLNDYQAEYGQSGGATIIETMKSGTQAFHGEAYYYNRNEDYNANDTFNKN
                     QIDATHPNGQPRPLYRFNTVGYNVGGPIFWPGKFNTSKNKLFFFFSQEIWPSKTPGTT
                     EYFMMPTALEKQGDFSQSVDKQGDVVTLTDPKNCGPSGTSACLLDPKHVNPLYIDPNT
                     QALLNVLPTGNATPLGDAPSGGKYNYVLQSPVKKPVNQQALRVDYNVTEKLHMFVHAM
                     RMTNNTEGPTNSPGFSASGEWGPSYFYDTPARNLAIGITYAATPSIVNEFTAGYADWR
                     ERSGFVNPTDMKKVQRGSSTGVNLGQFDPQNNPLNLVPNASFGGGSTNASSGFGISNA
                     PAINFYTRFPFNNNTGTWEYTDSLTKVWNKHISKFGIFWQNGRYVQHPIGNQFNGSFN
                     FGVNTSDVTDAGYAYANALLGNFQSYVEGTRTVYAPKWKMLEWYAQDNWKLTRNLTLD
                     YGVRFSYDFPYTLQPGDGVSFVPSRYNASQVPALYLPVKYSTLSGAQQTACAAGTKKA
                     PSTCAQNPDDPSDVQPSGAIGDFVGPFNYTGSVINTDPTYPRSLRNSNGVLVGPRLGV
                     SWDPFGDGKTAIRAGAGIFYNLREDAGVVGDFATQAPVVSNVNVNLGNVSTFTPGCDS
                     APGGCANISSNLAPQNTLLMPTNHKIASTMSANFGIQRDIGFATVIDVAYVGTFGRHL
                     EVTPNINEVPYLSEFQPQNLDPTKGVVTSLNGTVKQQGAISDNFFRPYPGYGTISDRE
                     YTGTSNYNALQATANHRLSRNLEFGVSYTWSKTMSYADTSSTGTAGSIATYLNPRFWN
                     YGEANIDRTNDFVGHWVYDLPKFSNMWNTKVVRALADGWEWSGVVSFISGAPQQVSIS
                     LSGVNFTGGGDGTRILLFGSPYALPNSGKFQYLNPANFALPPVGSTAPGVINPAAIPS
                     PSMPGITGRNTYIGPGTNQWDMTLVKNFHLTERFGLQIRGEAYNVFNHVSYNSVQDTA
                     SYNSATGTLTTNGNFGQMNGDLGPRILQLVGKFTF"
     gene            6159..7637
                     /locus_tag="VN788_06555"
     CDS             6159..7637
                     /locus_tag="VN788_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20224.1"
                     /translation="MNRTQVPGTIGLGGPPPEDIQKQLSKIIHSKAFRHAVALQRLLQ
                     YLISKTLEDPFADIKEYTIGVEVFERGPDYDPQSDTIVRVQIHRLRLKVKEYYETEGA
                     ADPILVDIPKGHYIPTFELRKGSSRSNTEVALTQHKNEEVDLKEFPAAVLPQSQAPRA
                     TTQFFVLRPSVLVLAVLIFATGVFIGTHWQLWRGGASIGRTSNGAPSDAARDFWRGFL
                     GNDNSPVVGYADAVYLVDGAEDLFRFRRGASDYTGTVVDPHLAQQFASSPALVAKAGP
                     LYYEDGNTGTGDLQSVFVLTKLFTQMGLQMLVKRCRLITIDDLQQHDVILLGSPEEND
                     AVAQLTRPSDFVWNRLDVADPWKGEYVNRHPQPGESASYKTERDPNTQEIKTDFGLIT
                     LQPGAVPNRRIVMLGGLDTSGVAGAAQFVTSPMHMAELRSRIESMGAWNGEGVPPSFQ
                     ALLRVDVERGNDVFDVHLISVHITNLQTPGSAASTAIAQSKQ"
     gene            7844..9556
                     /locus_tag="VN788_06560"
     CDS             7844..9556
                     /locus_tag="VN788_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014222101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004650 - polygalacturonase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 28 protein"
                     /protein_id="HXU20225.1"
                     /translation="MDSNQFFPRRKWLEMAAGASIGAGLLASTGMAAEPPAGSTKRDY
                     DLGTRTYNIRDYGAKGDGKTLDTEALQAAIDACNKDQGGTVLVPAGVFVIGTVELKSN
                     VRLHLAAAGKLLGSADGKRYHAASKIPLRGDSTLEDGNVGLLYAVEAENITIDGPGTI
                     DGQGAQFHSPTRGVLPPAGISGNQRPYHLLFYQCKNLNLRDIFLYDSAYHSVRVIQSS
                     FVKADGLRIYNRVNGNNDGFHFISSQYVAVSNCTVQSQDDACALFGSCKYVTITNSSF
                     STRWSVFRFGGGQAENIAVSNCIIYQAYGCPIKIQGGPNSRFENLSFSDLIMNRVTGP
                     ISIGVGARRRQGPDLAAQPANGSPTASVPDGIARNISFRGIRATVVNGEDLPDVPFPS
                     NFRPGERKSCIDLNAINDGVLENISFNDCQFTFPGGGTDQEAALRDVPNVAGEYFEIG
                     VLPAYALYARNVRGLTLGNVRFGVSEPDMRPAVVFDHVQDALINGFGAEGNTKAESLL
                     RFIDTQDVLMSASRVLTPAQVFLQVEGGDSKGITVDGGDISKAAKPVAYHSGALDSSV
                     KLRL"
     gene            complement(9950..>10136)
                     /locus_tag="VN788_06565"
     CDS             complement(9950..>10136)
                     /locus_tag="VN788_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXU20226.1"
                     /translation="AFTNGMPMTSSMGCGIWGGNITNENISLKHYMQVTWVSRPIPED
                     RPSEAELFGDFYNTETF"
BASE COUNT         2214 a         2772 c         2853 g         2297 t
ORIGIN      
        1 aacgcgcatt tggagggtcg cagaagaact tgaatacggg attgtgggaa tcaacacagg
       61 tctcatttcg accgaagttg ccccattcgg gggcgtaaag cagtcggggc tgggtcgcga
      121 gggatcgaag tacggaatcg aggagtttct agagattaag tatttgtgct taggggggat
      181 gaacttctag tgatcgtcca cgataaggat ctcgattttc ggcatctttt taggaaaggc
      241 ggcggcatcg ctccattcga aaattgaagg cgtaaaattt ctaacatcgc agcccaatag
      301 caaaaacctg gtcaatgcct tcggtcaata tctgccaagc gccgaaagcg cagaatacct
      361 ctccgatctc ccctttcaac ggggaaacgc cttcccaatc gcctcaagtt gatccataaa
      421 cgcgttcgaa atcgccccat ccatcgcgac gggaacgtcc caagtcaccg ctcctcccca
      481 atcccgcact ttctttgtcg ccgcgatcgc ctgttccgtc gaaaagcgcg gctcgcccct
      541 accccaatgt tctcctagaa aacagagcat atgaatctgg ctcccatcga tgcgcccatt
      601 tgcctgatgc atcgctttca ccagatttgg atcgtcgatt tctcccgccg tgaaatcctc
      661 gtctggcgac atcgcaaaga gccggttata cacacccgga ttgaacgcga cgcacgcatc
      721 ctggtttccc acacgcgctg ccgccgcgag gcttgtgaag ttcggtggct ctggcgaacg
      781 atacatcgaa tccgggtaaa cgaccccatc gagccaccag cccgcgatcc gtttgcccca
      841 gcgctgcgac cattcgcgga tgatcgcttc ccatttccgc tgaaaattct tgttcggata
      901 gccgccgctc tgccattcga gcgcatcctt tgcgaccttg tcaccgttcg gcgcgcccga
      961 aggcaagtaa accatcaatt tgatatcgcg ctgccgcagc ggctcataaa aatccgcgac
     1021 cagatcgcgg cgcgaacatt tgctcggcgc gattcctgtg gttttgtcat aggttgcatt
     1081 tggcgagagg tagtagccgg agttttgccc gatactgagc tggtaatgcc ccgccccgac
     1141 cgatgcgaga cgcttggaca taccctcgac gtcccatgcg tcgatcatct tgttccaccg
     1201 gtccacgttc atgtcgagct tgttgacctg cgattgccaa tcggccaggt aatgcgtcat
     1261 cacgccccag gcgtagcggg gattctgcat ccagtccgcc cgccgcgtgc cttgataaag
     1321 ctgctgccca tccggcgccg tcatcggctg cggcggcgac gactgttcgc gcccgccgac
     1381 cgattgaagc ggtgcctgcg cccgcgaccc cgcgtctccc ggctcggcga acgccgcggc
     1441 ccccaacgct gccgtcgaac ttgctatcca ctctcgccgc gaaatctttt tcgtactcat
     1501 tgcttgcccc caagagctca ccgatggcat caacttagca cgccgcgccc gtgaagcgaa
     1561 tctcaaaccg ttgcgttcgc tgtcgattag aaatttgtat ctactggaaa ggtgaggctt
     1621 acggcgggtt tcaatcgcga gactgtcgat tagaaagcgt gactccaatt tttgattgtg
     1681 tattcgcgat gaaggcattg cgtgatcgca tgtttcgtcc ctccgctagc taccgcgatt
     1741 agagctggtc atcgtccttt acttgcaatg agttacattg agttttaatt ctttgcagtt
     1801 gcgattagaa cgacaaattc caaatatcgg gcgatacgtt tcggatctga ttttcctgtt
     1861 cgaggtggtg ggtcattgtt ggctgcctgc tgtcggcgat gccatgaaaa tccaaatgtg
     1921 tcgtcaacaa aaaaacaagc cgccacggcg ggtggggacc gacgtgacgg cttactacgc
     1981 gcgcaaatcg tttgacgcag taaaggccca gcaagccgcg ctctaagagg cgtcagccta
     2041 aaacgtgaat ttgccgacga gttgcaaaat ccgcggcccg agatcgccgt tcatctggcc
     2101 gaagtttccg tttgtggtca gcgttcccgt cgcagaattg taacttgcag tatcttgaac
     2161 cgagttgtag ctcacgtggt tgaacacatt ataggcttcg ccgcggatct ggagaccgaa
     2221 acgctcggtg aggtggaaat tcttcaccaa tgtcatgtcc cattgatttg tgcctggccc
     2281 gatatacgtg ttgcggccgg tgatacctgg catgctcggg ctcggaatgg cagctgggtt
     2341 gatcacgccg ggcgcggtgc tcccgaccgg cggcaaagcg aaattcgccg gatttagata
     2401 ttggaatttg ccactgttcg gcaaagcgta ggggctcccg aagaggagta tgcgagtgcc
     2461 gtcaccaccg ccggtaaagt tgacgccact gagactgatg ctgacctgct gcggcgcccc
     2521 gctgataaat gagacgacgc ccgaccattc ccatccatcc gctaacgcac ggacgacttt
     2581 ggtattccac atattactga atttcggtag gtcatagacc cagtggccga cgaaatcgtt
     2641 ggtgcggtcg atgttcgcct caccgtaatt ccagaagcgt ggatttaggt aagtggcgat
     2701 cgagccggcc gtgccggtgg acgaagtgtc cgcgtaggac atcgtcttgg accacgtgta
     2761 ggagacgccg aactcaagat ttctgctcag ccggtgattc gccgtggcct gcagcgcgtt
     2821 gtagttggac gtgcccgtat attcgcggtc gctgatcgtg ccataccccg ggtagggccg
     2881 gaagaaattg tcgctaatgg cgccctgttg ctttaccgta ccattgagac tggtgaccac
     2941 acctttggta ggatcgagat tctgaggctg gaactcggaa agatacggca cctcgttgat
     3001 gttaggagtg acctccagat gacgaccgaa ggtaccgacg taagcgacat caatcacggt
     3061 tgcaaatccg atgtcgcgct ggatcccgaa attggcgctc atggtcgagg cgatcttgtg
     3121 attcgtcggc atgagtaaag tgttctgtgg agcgaggttc gaggagatat tagcgcaacc
     3181 gccgggagcg ctatcgcaac ctggcgtgaa ggtagaaacg ttcccgagat tcacgttgac
     3241 gttgctgacg accggcgcct gcgtggcgaa gtcacccacg acgcctgcgt cttcgcgaag
     3301 gttgtagaaa atgcctgctc ccgcgcgaat ggcggtcttg ccgtcgccaa aggggtccca
     3361 tgacactccg aggcgcggac cgactagcac gccattgcta tttcgcaaag aacgcggata
     3421 tgtcggatcc gtgttgatca cactgccggt gtaattgaat ggccctacga agtcgccaat
     3481 cgcaccagag ggttgcacat cactgggatc atcaggattt tgtgcgcaag tggaaggtgc
     3541 ctttttcgtg cccgcggcac aagctgtctg ctgagcgccg gacaaggtgg agtacttgac
     3601 aggaagatag agtgccggaa cctgcgatgc attgtatcgg ctcggcacaa agctaacgcc
     3661 gtcgccgggc tggagggtgt atggaaagtc gtagctgaag cgcacgccat aatcgagcgt
     3721 gaggttgcgc gtgagcttcc agttgtcctg cgcgtaccat tcgagcatct tccattttgg
     3781 cgcatacacg gtgcgagtgc cttcaacgta actctgaaag tttccgagga gcgcgttcgc
     3841 atatgcgtaa ccggcgtcgg tcacatcaga tgtgttcaca ccaaaattaa acgaaccatt
     3901 gaactgattg ccaatgggat gctgcacgta gcggccgttc tgccagaaga tgccgaattt
     3961 cgagatgtgc ttgttccaga ctttcgtgag gctgtctgtg tattcccacg tgccggtgtt
     4021 gttgttgaat ggaaagcgcg tgtagaagtt aattgccggc gcgttggaaa taccaaaccc
     4081 ggaactcgcg ttggtgctgc ctccgccgaa gctggcatta ggaacgagat tgagcggatt
     4141 attctgagga tcaaactggc cgaggttgac tcccgtcgaa gagccgcgct gcaccttctt
     4201 catatcagtg ggattgacga aaccggaccg ttcgcgccaa tcagcgtacc ccgccgtaaa
     4261 ctcattgacg atcgatgggg tcgcagcgta ggtgatgccg atagcgaggt ttctcgcggg
     4321 cgtgtcataa aaatatgaag gtccccactc gcctgaagca ctgaacccgg gagaattcgt
     4381 cggcccttca gtgttattgg tcatgcgcat tgcgtgaacg aacatgtgca gtttttcggt
     4441 gacattgtaa tcaacgcgca gcgcctgctg attaaccggc ttcttaaccg gcgattgaag
     4501 aacatagtta tatttgccgc cgctgggagc gtcacccaag ggcgttgcat tccccgtcgg
     4561 aagcacgttg agcagcgcct gcgtattcgg atcgatgtaa agcggattga catgcttggg
     4621 atccagtagg caggccgacg taccgctggg gccgcagttt ttcggatcgg taagcgtcac
     4681 aacatcgcct tgcttatcga cgctctgcga aaaatcgccc tgcttttcga gcgctgtggg
     4741 catcatgaag tattctgtag tcccaggagt cttgctcggc caaatttcct gcgagaagaa
     4801 gaaaaagagt ttgttcttgc tcgtattgaa cttgccaggc cagaagatcg ggccgccaac
     4861 gttgtaaccg accgtgttga aacgatagag ggggcgcggc tggccattag ggtgcgttgc
     4921 atcaatttgg tttttattga aggtgtcgtt tgcgttgtaa tcctcgttgc gattgtaata
     4981 gtaggcttcg ccatggaatg cttgcgtgcc cgacttcatc gtctcgatga tggtggcacc
     5041 gccgctctgg ccgtactccg cctggtaatc gttcagcagc actttcacct cgccgacggc
     5101 ctcactattt agaggagtat cgaagcgatt accgccgcca cgcggattgc cgttgacgcc
     5161 gtctacgctg accgagttac tcacttcccg cgtccccgaa acggcgccgg cgctttcggt
     5221 gccgagctgt gaggcacctt cgccgtcttt cacaacgcct ggcagcagcc gcaccaaggc
     5281 gagcacatca tgccccggcg tgctgatatg ctcgagttcg ttactatcga gcagtgccga
     5341 gcgctcggcg ctgtccgttt gcaccggggt tggtgtggcc gtaacgctga ctgactggct
     5401 gactgcgcct aattcgagct ttagggttcc cgcggagagc cgctgcgacg cactgagact
     5461 caaaccactt ttttcgagct tcttgaagcc ggtggcctcc actgaaaccg caaattggcc
     5521 gggctgcaca tcaacaaagg caaactgacc ggagtcctgt gtttttgcgg ctctcgtgtc
     5581 gccagtaagc tgatttgtga gcgtcaccgt cgcgccgacc acagcggcgc ccgtcggatc
     5641 gacaacttgg ccggaaatct cgccgctggt cgtctgcgca tgaattcccc cggcagcaat
     5701 caccaaaagc aaaagaaata acgcaagcgc tttccacaac cccacggaca atcgcataac
     5761 ttctactcct cccctggatt ctttagccga atggttcgtt ttcctgtttg ccgccccaca
     5821 actttttgcc gttaggcagc cttatgaaaa ggaacgctgt ctgacggctg cacgccccac
     5881 taaaactgaa aatggagtcc aggctgacgc cacctgctga gaatcgaagt taattgggta
     5941 acgtcgaatc ccgcgccgtt cgacgtgcgg cagcgtcgcg actcataccc cttgttttct
     6001 gtgcggaatc taggactgta cgcagagaac ctgcaagtta attgaggata cggtaccgct
     6061 acgcgaaagc tacatagaag atacacggca gatacctccc gatgttataa tccggcagtt
     6121 ttgcctgcgc gccgggcggg tccgggggaa tgttcgtcat gaacaggacg caagttcctg
     6181 gaacaattgg acttggcggt ccgccgcctg aggatattca gaagcaactc agtaagatca
     6241 ttcattcgaa ggcgttccgc cacgcggtcg ccctgcagcg cctgttacag tatttaattt
     6301 ctaagacgct cgaagatccc ttcgctgaca tcaaggaata cacgatcggc gtggaagtct
     6361 tcgaacgcgg gcccgactac gatccgcaga gcgacacgat cgttcgcgtc caaatccacc
     6421 gtttgcgcct gaaggtcaag gagtattacg agacggaggg agccgcggac ccaattctcg
     6481 ttgatattcc caaaggccat tacatcccca catttgaact cagaaaaggg tcgtcgaggt
     6541 cgaacacaga ggttgcgctg acgcagcata aaaacgagga agtggacctt aaggaatttc
     6601 cagctgctgt tcttccgcaa tcacaggcac cgcgcgccac cacccaattc tttgtgcttc
     6661 gtccctctgt actcgtgctt gccgtgctga ttttcgcgac gggagtcttc attggaacgc
     6721 attggcagct gtggagaggc ggtgcttcca tcggccgaac ctccaatgga gcgccgtctg
     6781 atgcagccag ggatttctgg cgaggctttc tcggaaacga taactctcct gtggtgggat
     6841 acgcagacgc agtctacctc gtggatggcg ctgaggattt attcaggttt aggcgcggag
     6901 cgagcgatta caccggaacc gtcgtagatc ctcatctggc tcaacaattt gcatcgagcc
     6961 cggcgctggt ggcgaaagcc ggtcctttat attacgaaga cgggaatacg ggcacaggcg
     7021 accttcaaag cgtgttcgtg ctcaccaagc tctttacaca gatgggtttg caaatgctcg
     7081 tcaaacgttg tcgtctgatt acaatcgacg atctgcagca gcatgatgtg atcctgctgg
     7141 gatctccgga ggaaaatgat gccgtggcac agttgacgcg gccttcggat ttcgtgtgga
     7201 acaggcttga tgtggccgat ccatggaaag gtgaatacgt caaccgacat ccgcaacccg
     7261 gcgaaagcgc ttcctataaa accgagcgcg atcccaatac gcaagaaatt aagaccgact
     7321 ttggcctcat tactttgcag ccgggtgctg tgccgaaccg gcgcatcgtt atgcttggcg
     7381 ggcttgatac ttcaggcgtc gctggcgcag cgcaattcgt gacttcgccg atgcatatgg
     7441 cggaactaag gtcgaggatc gagtcgatgg gcgcatggaa cggagagggc gtaccgcctt
     7501 cgtttcaggc gctgctgcgt gttgacgttg aaaggggcaa tgacgttttt gacgtccacc
     7561 tgatttcggt ccacataacg aacttgcaga cgcccggctc agccgcctct accgcgatcg
     7621 ctcaatcaaa acagtagtaa caccgggtat ctgtcgtgta actttccggt atccctaatt
     7681 aacttgaaca agaggctgag gcggaactat attccgcgcc gacgattcgc acatggccgt
     7741 gaactaagac cgggttgtac aacccaacac gttgaacccg cccaagcgcc tacaatttca
     7801 gatgtgcggg ttggcgtgca gaacggagat ccaaaactaa gctatggact caaaccagtt
     7861 tttcccgcgt cggaagtggc tggagatggc ggcaggagca tcgatagggg ctggactttt
     7921 ggcgtcaacc ggcatggcag cagagccgcc ggcaggttcg acgaaaaggg attacgatct
     7981 cggtacacgc acgtacaaca tccgcgatta tggcgccaag ggcgacggta agacgctgga
     8041 taccgaagcg ctccaggccg cgattgatgc gtgtaataag gaccagggtg gaacggtgct
     8101 cgtcccggcg ggagttttcg tgatcggcac cgtcgaactg aagagcaacg tccgattgca
     8161 tctcgcggca gcgggaaagc tgctgggcag cgccgacggc aaacgatacc acgccgcaag
     8221 taaaatcccg ctaaggggtg actcgactct agaagacgga aacgtcggtt tgctttacgc
     8281 ggtggaggca gaaaacatca cgattgatgg tcctggaact atcgatgggc agggcgcaca
     8341 atttcattcg ccgactcgcg gtgtattgcc gccggcgggg atcagcggca atcagcggcc
     8401 gtatcatctg ctcttctatc aatgcaaaaa tttaaatctg cgcgacattt tcctctacga
     8461 cagcgcctac cattccgtcc gcgtgattca aagctcgttt gtgaaagccg acggtctgcg
     8521 catttacaac cgcgtgaacg ggaacaatga cggcttccat ttcatcagca gccagtacgt
     8581 ggcggtgagt aattgcacgg tgcagtcgca ggatgacgct tgcgctcttt tcggaagttg
     8641 caagtatgtg acgatcacaa actcgtcctt cagtacgcga tggtccgtgt ttcgctttgg
     8701 aggcgggcag gccgagaata ttgccgtatc gaactgcatc atttatcagg catatggctg
     8761 ccccatcaag atacaaggtg gtccaaactc gcgttttgaa aacctctcat tttcggatct
     8821 cattatgaat cgcgtgaccg gaccgatctc gattggcgtc ggggcgaggc gccgacaggg
     8881 gcccgatctc gcagcgcagc ccgcgaatgg ttcgcctacg gcatctgtgc cggacggaat
     8941 tgcgcgaaat atttcgtttc gcggaattcg cgctacggtt gtaaatggtg aagaccttcc
     9001 cgacgtacca tttccaagca atttcaggcc gggcgagagg aaatcgtgca tcgatttgaa
     9061 cgcaattaat gacggcgttc tggagaacat cagctttaac gattgccagt tcaccttccc
     9121 cgggggtggg acagaccagg aagccgcact gcgcgatgta ccaaacgtcg ccggcgaata
     9181 cttcgaaatc ggcgtgctcc ctgcgtatgc tctgtatgcg cgcaacgtgc gtggcctgac
     9241 gctcggcaat gtgcgatttg gcgtgagtga gccggatatg cgcccggcgg tcgttttcga
     9301 ccacgtgcag gatgcactca tcaacggctt cggcgccgag ggcaacacga aggcggagtc
     9361 gctcctgcgc ttcatcgata cgcaagatgt gctgatgagc gcatcacgcg tcctaacacc
     9421 ggcacaagtc tttcttcagg tagaaggcgg agacagcaag ggcatcacgg tggacggggg
     9481 agacatatcg aaggccgcga agcctgtggc gtatcattcc ggtgcgctag acagttccgt
     9541 caagctgcgg ttatgaacac ctgtgccttg gtcgactttg gaaaaagtac ttgacacgcc
     9601 cgtgctcatt ctcatagtta acacaatctg gttgtgcatt cccctcgcgc atgctcccct
     9661 gaaacaacac aacattggac gttcgaaaca cccatgtatc ccgcatctcg ctcattgggg
     9721 cgggaagcaa gtttcgagga tggcgccgtt caacgctttc acaggtggtg cacagcaaaa
     9781 gcggtgcgct ttgtgatcta gttcaaaagc tgttgtgcaa aaggaagctg gaaccgacac
     9841 aactcaaacc gtcgttgtaa acctggcaag actgttctat atgtgcgacc gaaaaatccc
     9901 gcctcgggat tgggagcttt ggcatggagt gagttgttct ggccgatact caaaatgtct
     9961 ccgtattgta gaaatcgccg aagagttcgg cttccgacgg gcggtcttcg ggaatcgggc
    10021 ggctcaccca ggtgacttgc atatagtgct tgaggctgat gttctcattc gtgatattgc
    10081 cgccccagat gccgcagccc atgctcgagg tcatcggcat gccgttcgta aacgcg
//
LOCUS       DATNDT010000201         3593 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_834635, whole genome shotgun sequence.
ACCESSION   DATNDT010000201 DATNDT010000000
VERSION     DATNDT010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834635"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1023)
                     /locus_tag="VN788_06570"
     CDS             complement(<1..1023)
                     /locus_tag="VN788_06570"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU20227.1"
                     /translation="MAHLLIVDDEPSIRELLEISFRKEGHKVEVAANGDAAKRQMASR
                     MFDIVVSDICMPDMDGVELLRYCKEVSPETVFILITGVPTIGTAIDAVNFGADRYVIK
                     GDRLLDELRPAVHHVAESMALRKEAGDLRRELRRLTGLDHMIGTSPKMRSIFELIQNV
                     APQSSRVLITGESGTGKELVARAIHENSARAKSPFVTINCGAFPETLLESELFGYVKG
                     AFTGANDNRRGLFHAAHTGTLFMDEIGNMSVTMQVKLYRVLQEGKVRPIGSTEEIDVD
                     VRVIAATNKDFEKEIAEGRFREDLYYRLSVIPIHLPPLRERRDDIPLLAREFLGRFAK
                     SMSKNIE"
     gene            complement(1033..2709)
                     /locus_tag="VN788_06575"
     CDS             complement(1033..2709)
                     /locus_tag="VN788_06575"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU20228.1"
                     /translation="MEPTPSISEWLPWLGRVRFLVITFLVAGIIAVRQWTPSPLPLRT
                     LLGLSVLWYVLATLYLLMQRALPRSRWHAPFQMAVDLGVITGVVYASGAQDSYFISLY
                     LLAILMASILFSRRGTFLVAGFSFVLLGCITELVYYGIIPRTAISTPSLRALESWLGS
                     NLFAFFAVAYLGSLLAQTVQSKGVELEEKSEALKDLQAFNNDIIESMRGGLLTVDPEG
                     RILLINRAGAEITGHGFGLLRGENVADVFPGFWPVEMDEAGIPRALRKEVEIQTPGGA
                     TRFLGISISPLRTRQNQASGFVFNFQDLTELKRLEHEVFTKERMAALGRLSAAIAHEI
                     RQPLTAMTGALKELARFAPLEDDDKKLVQIVSRESQRLNQIITEFLDYSREKSYSFAD
                     VDILSLIEDTLVLIERNAVASGGVRIERHFAERPLRARADRDAMKQVFWNLCNNALRA
                     MPDGGVLSVRVEAVKNSVRISIRDTGRGMEPAASTRIFEPFQSGFPGGTGLGLAIVYQ
                     ILQAHRGRIHVETGLGSGAEFIVDLPAAAGAGVIASALAGGTALQAAGKG"
     gene            complement(2834..>3593)
                     /locus_tag="VN788_06580"
     CDS             complement(2834..>3593)
                     /locus_tag="VN788_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HXU20229.1"
                     /translation="IEKNVKLKRAVKSAMIYPISVLSIAAGVIILLLWKVVPIFITLF
                     NGLDVSLPLPTRIVIGLSHFVGSVYGLMILIFGIACGFALKFWYGTPQGRMAIDTVIL
                     KLPVVGIVMRKIAVARFTRTLGTLISSGVPILEGLDITARTSGNAVVEKAISETRKAV
                     EAGRSLVDPLKETDVFPGMVTQMIGVGEQTGAMDAMLSKIADFYEDEVDAAVKDMLTA
                     IEPIMIVVLGVIVGGVVISMYLPLFSLIGKLAGH"
BASE COUNT          792 a         1077 c          983 g          741 t
ORIGIN      
        1 ctcaatgttc ttggacatgg acttcgcgaa acgccccaaa aattctcgcg cgagcagagg
       61 gatatcgtcg cgacgttcgc gtagcggcgg aaggtgaatg ggaatcacgc tcaggcgata
      121 atataagtcc tcgcgaaagc gtccctcggc aatttccttc tcaaagtcct tatttgtagc
      181 ggcaatcacg cgtacatcca cgtcgatttc ctcggtactc ccgatgggcc ttacttttcc
      241 ctcttgcagc acgcggtaga gcttcacctg catagtcacg ctcatattgc caatctcatc
      301 cataaagagc gtgcctgtat gagcggcatg aaaaagcccg cgccggttgt cgttcgcgcc
      361 tgtgaacgcg ccctttacgt agccgaacag ctcggattcc agcagcgttt ccgggaaagc
      421 accgcaattg atggtgacga acggactttt ggcccgtgcg ctattctcgt gaatggcgcg
      481 cgccaccagt tcttttcccg ttccgctctc gccagtgatc agaacgcggc tggattgcgg
      541 cgcgacattc tgaattaact cgaaaataga gcgcattttc gggctggtgc cgatcatatg
      601 atccagtccc gtgaggcgcc ggagttcgcg gcgtaaatcg ccggcttcct tccggagcgc
      661 catgctctcc gcaacgtggt gaacggcggg ccgcaactca tcgagcagcc ggtcgccttt
      721 tatcacgtag cgatcggcgc cgaaattcac ggcgtcgatg gccgtgccga tggtgggaac
      781 tccggtaatc aaaatgaaaa cggtttccgg cgaaacctct ttacagtagc gcaagagttc
      841 gacgccatcc atgtcgggca tgcaaatgtc ggagacgacg atatcgaaca tgcgcgacgc
      901 catctgccgc tttgccgcat cgccgttggc ggcgacttcc actttgtggc cctctttgcg
      961 gaagctaatc tcgagcaact cgcgtatgga gggttcgtcg tccacgatca agagatgagc
     1021 catatcggtg ctttaccctt ttcccgctgc ttgcagggca gtcccgcccg cgagcgcgct
     1081 agcaatgacg cccgcgccag cggcggcagg caggtccacg ataaattccg ccccgcttcc
     1141 gagccccgtt tccacatgaa tgcgtccccg gtgcgcctgc agaatctgat aaacaatagc
     1201 gagtcccaac cccgttcctc ccggaaaccc cgattgaaac ggctcaaata tgcgcgtgga
     1261 ggcggccggc tccattcccc gcccggtatc gcggatcgag attcggacgg agttcttaac
     1321 ggcttccacg cgaactgaaa gcacgccgcc atcaggcatc gctcgcagag cgttgttgca
     1381 aaggttccag aagacctgtt tcatcgcgtc gcggtccgcg cgggctcgca gtggacgctc
     1441 ggcgaagtgg cgttcgattc gaacccctcc ggacgcaact gcgttgcgtt cgatgaggac
     1501 gagcgtatct tcgatgaggc tgagaatatc cacatccgcg aaggagtagg acttttcgcg
     1561 cgaatagtcc agaaactcgg taatgatttg attcaggcgc tgcgattcgc ggctgacgat
     1621 ttgaacgagc ttcttatcgt catcctcgag tggcgcgaaa cgcgccagct ccttaagcgc
     1681 ccccgtcatt gccgtaagag gctggcgaat ttcgtgggcg atggccgctg agaggcgccc
     1741 cagagcggcc atgcgctctt ttgtaaaaac ctcatgctcg aggcgcttca gctccgtcaa
     1801 atcctggaag ttaaagacga atccactggc ctgattttgc ctcgtccgca gtggagaaat
     1861 tgaaatgcca agaaatcgcg tggcgccgcc cggagtctgt atctccactt ctttgcgcag
     1921 tgctcgcgga atccccgctt catccatttc caccggccag aagcccggga aaacgtctgc
     1981 aacattttcg ccgcggagca gtccgaatcc gtggcccgtg atttccgcac ccgcgcgatt
     2041 aatcagtaaa atgcggccct caggatctac ggtaagcaag ccgccgcgca ttgactcaat
     2101 gatgtcgttg ttgaaggcct gcaaatcctt caacgcctcg ctcttttcct ccagctcgac
     2161 gcccttgctc tgaaccgtct gcgccagcag gctgcctaga tacgccacgg cgaaaaatgc
     2221 aaagaggttg cttcccagcc aggattcaag cgcccgcaag ctcggagttg agatcgcagt
     2281 gcgcgggatg attccgtaat agacgagctc ggtgatacac ccgagaagca cgaagctgaa
     2341 tcctgccacc aggaaagtgc cgcgtcggga aaacaggatg ctggccatca gaatcgcaag
     2401 caagtacagc gagatgaaat aactgtcctg cgctccgctg gcatagacga cgcccgtgat
     2461 gactcccagg tccacggcca tttgaaaagg ggcatgccat cggcttcgcg gaagggcgcg
     2521 ctgcatcagc aaatacagag tcgcgaggac ataccataag accgataagc cgagcagcgt
     2581 ccgcaaggga agtggcgagg gggtccattg gcggacggcg atgatacccg cgaccaggaa
     2641 ggtaattacg agaaagcgaa cgcggcccaa ccaaggcaac cattcactta tgctgggtgt
     2701 gggctccatc aaggatccga taggtgctct cctcgcttga tcggagtgcc ccggcgatct
     2761 cgcatttcga tttggcgcgg ccgcgagagt agtccgattc gagatttcgc aatcgcggcg
     2821 ttgcgcgagc gggttagtgt cccgccagtt tgccgatcag cgagaatagc ggcaaataca
     2881 tcgaaataac cacgccgcct acgatcacgc ccaacaccac gatcatgatc ggctcgatgg
     2941 ctgtcagcat atctttcacg gcggcgtcca cttcgtcttc gtagaagtcg gcgatcttcg
     3001 aaagcatggc atccatcgca cccgtctgct cgccgacccc gatcatttgc gttaccatcc
     3061 ccggaaaaac gtccgtttcc ttcagaggat cgaccagcga acggccggct tcaacggcct
     3121 tccgcgtctc cgaaatcgct ttttccacaa cggcgttgcc ggatgtccgc gccgtgatgt
     3181 ctagtccctc gagaatgggg acgccgctcg aaataagcgt gcccagcgtt cgcgtaaatc
     3241 gggccaccgc gatttttcgc atgacgattc caaccaccgg aagtttaaga attaccgtgt
     3301 cgatggccat gcgcccctgc ggggttccgt accaaaattt cagcgcaaag ccgcatgcga
     3361 tgccgaagat caatatcatc aacccgtaaa cgctgccgac gaagtggctc agtccgatca
     3421 cgatccgggt gggcagcggc agagacacgt ctaatccatt gaataacgtg atgaaaatcg
     3481 gaacgacttt ccagagcaac aaaatgatga cgcccgcggc gatgctcaaa accgaaatcg
     3541 gatagatcat cgcggacttc accgcccgct tcagcttcac attcttttca atg
//
LOCUS       DATNDT010000202         4383 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3761641, whole genome shotgun sequence.
ACCESSION   DATNDT010000202 DATNDT010000000
VERSION     DATNDT010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3761641"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..928
                     /locus_tag="VN788_06585"
     CDS             <1..928
                     /locus_tag="VN788_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXU20230.1"
                     /translation="AVVGCGAIGAASSSLLARAGVGTLQIIDRDYVEASNLQRQVLFD
                     EEDARQSLPKAEAAARHLSSINSEVHVDAVVKDLTPANIDLLTNAHLILDCTDNFETR
                     YLLNDFAVNSRLPWIYAGAVASQAVTMSVVPEDTACLACIFPEPPRGNQMTCDTAGIL
                     NSTANLVACIQVTEALKLIIGARDQVRRTLLSLDLWTNQRSEVSTAAPRSDCRCCRGR
                     VFDHLDGKLRPQVTMCGRNSVQIHERERPIDFSEMNSRLAPHGQVRHNEMVLKFWRDA
                     YEMTLFRDGRAIIKGTTDPAVARSLYARFVGS"
     gene            1019..2155
                     /locus_tag="VN788_06590"
     CDS             1019..2155
                     /locus_tag="VN788_06590"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXU20231.1"
                     /translation="MSNKQTTPEDLAGAVAAEELNQPFDRIDLPIQPPFPVMEARVAA
                     QLPETGDWQYEPKWDGFRGLAFRRGNAVLVQSKAGQPLGRYFPELIEHLLALPVEQFV
                     LDGEIVILSEGRLDFNALLQRIHPAESRIRKLAKETPVTFLCFDLLVNAAGELLADRP
                     LTERRKALEDFFKKIPNSEGLWLSPATRDLGAAQRWMKDLAASGFDGVMAKRLGEPYH
                     SGDRKAMLKIKRHRTADCVVGGFRIASKGGQVGSLLLGLYDQAGKLNHVGFSSSFNAE
                     ARGKLLETLKPFVVEEKQGEGVGFSGNAPGGPSRWSTERSTEWTPLRDELVCEVQYDH
                     FSGGRFRHGCKFLRWRPEKSPRQCTYEQVQPATAGDLGGLLKTA"
     gene            2281..2967
                     /locus_tag="VN788_06595"
     CDS             2281..2967
                     /locus_tag="VN788_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HXU20232.1"
                     /translation="MLTSSAILFDLDGVLVDSTPAVERVWRRWATEHGLDPEFVMQWA
                     HGRRSFETIRRVAPYIDAARENLKVERREIEDLEGVRIIPGAAELIASIPPGHWTIVT
                     SATRELARARLGHVGLPLPAEAITAEMVQNGKPDPEPYLKGAMQLAFAPKSCIVIEDT
                     AAGIASAKRAGMQAIALTTTYPAHELREADIIVRSCADIRVEVKSGDDDDTLLQIFFE
                     YAVEADVVIS"
     gene            3038..4111
                     /locus_tag="VN788_06600"
     CDS             3038..4111
                     /locus_tag="VN788_06600"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP kinase"
                     /protein_id="HXU20233.1"
                     /translation="MPTSSNHTIVAHASCRVDLAGSTLDLWPLYLFHPGAVTVNFAVN
                     ILTRCQITPNADSGIHFKSIDTGREDYFRDFEEFCRARKIQHHLAAHLVRFFFPLGTP
                     QDCGFTLETNSESPAGAGISGSSALMVATVAALARYTGRKLSREQVREIAQNVEAQLI
                     RVPTGCQDYYPALYGGVSAIRLDVDGIHRQAIAVAPENIESRFVLVYTGAPRQSGINN
                     WEVFKAHINGDQRVFRNFKQIGEIARAMHAALEAGDWKQVPKLLGEEWKLRRTNAPGI
                     TTPLMDKLISAAKRKGALAAKVCGAGGGGCFVLFVSEGSHDAVAEAIRQHGGRVLPFR
                     VNPDGVTVVGRNRRPAREGNPLR"
BASE COUNT          929 a         1257 c         1287 g          910 t
ORIGIN      
        1 cgcggtcgta ggatgcggag ccatcggggc cgcatcctcc agtttgctgg cacgtgccgg
       61 tgtcggaacg cttcagatta tcgatcgaga ttatgtcgag gccagcaatc tccagcgcca
      121 ggtgctcttc gacgaagaag acgctcgaca gagtttaccg aaagcggaag ccgcggcgcg
      181 gcatttgtcg agcatcaatt ccgaggtcca cgtcgacgct gttgtaaaag acctgacgcc
      241 ggcgaatatc gatttgctca ccaatgccca cctcatcctc gattgcaccg acaattttga
      301 gactcgatac ctgctgaatg atttcgcagt gaacagccgc ttgccctgga tctatgcagg
      361 tgcggtagct tcgcaggcgg tcacgatgag cgttgtcccg gaggacaccg catgtctggc
      421 ttgcattttt ccggagcctc cgcggggaaa ccagatgacc tgtgacaccg ccggaatcct
      481 gaacagcacg gcgaatctgg tggcctgcat tcaggtaacc gaagccctga agttgattat
      541 cggcgctcgg gaccaagtcc ggcgaacgct gctctcattg gatctgtgga caaatcagcg
      601 ttcggaagtt tccaccgcag cgcctcgcag cgattgccgc tgttgccgtg gccgtgtatt
      661 cgatcatctc gatggcaagc tgcggccgca agtcacaatg tgcgggcgaa actcagttca
      721 gatccacgaa cgggaacgcc ccatcgattt ctcggagatg aactcgcgcc tggcgcctca
      781 cggacaggtg cgccacaacg aaatggtgct gaagttctgg cgagacgcat atgagatgac
      841 gctctttcgc gacggtcgcg ccatcatcaa ggggactacc gatccggcgg tggcgcgcag
      901 tttgtatgcc agattcgtgg gatcgtgatc cgccatccgc tctggatgac agcggttctg
      961 gcatcagccc gccaaagcaa aaacatcttc gctgaccttc tcgcatctta atatccagat
     1021 gtcaaacaaa cagaccacac ctgaagacct ggcgggcgcc gtcgctgccg aggaactgaa
     1081 ccagccgttc gaccgcatcg atttgcccat tcagccgcct tttccggtga tggaagcgcg
     1141 cgtcgccgcg caattaccgg aaaccgggga ctggcagtac gaaccaaaat gggacggatt
     1201 tcgcgggttg gcatttcgcc gcggcaatgc ggtcctggta cagtcgaaag cgggtcagcc
     1261 gctgggacga tactttcccg aattaatcga gcacctgctc gcattgccgg tggagcaatt
     1321 cgttctcgac ggtgagatcg tgatccttag cgaggggcgc ctcgacttca acgccctgtt
     1381 gcagcgcatc catccggccg agagccgtat tcgcaagctg gcgaaagaaa cgccagtcac
     1441 cttcctgtgc ttcgatctgc tggtaaatgc ggcaggagaa cttctggctg acaggccgct
     1501 tacggagcgc agaaaagcct tggaagattt cttcaagaag attcccaaca gtgagggtct
     1561 gtggctttcg ccggcgaccc gcgacctcgg cgccgcgcaa cgctggatga aagatctggc
     1621 ggccagcggc ttcgatgggg tgatggcgaa gcgactgggc gagccttacc actccggcga
     1681 tcgcaaagcg atgttgaaga tcaagcgaca tcgcaccgcc gactgcgtag tcggaggctt
     1741 ccgaatcgcc agcaagggag ggcaagttgg atcgctgctg ctcggcctct acgatcaagc
     1801 cggcaagctg aatcatgttg gattcagctc gagcttcaat gccgaagcgc gcggaaaact
     1861 gctggagaca ttgaagccat tcgtcgtcga ggagaaacaa ggtgagggag tcggcttcag
     1921 tgggaatgcg cccggtggcc ccagtcgctg gtcaaccgaa cgctccaccg agtggactcc
     1981 gctgcgcgat gagctggtgt gcgaggtgca gtacgatcac ttttccggcg gacgctttcg
     2041 ccacggctgc aaattcctgc gctggcgccc ggagaagtcg ccacgccagt gcacatatga
     2101 gcaggtgcag ccagcgacgg ccggggattt ggggggccta ctgaagacag catgaaactg
     2161 attcgcgatt tcgtaatttt caatttcgta atcgggtaat ttgaaaatca agaaaattac
     2221 gaattccccg attgcaaaat tcgaaatcaa ttctcatgac tctttgatac tctttgccca
     2281 gtgctcacct cctccgcaat cctcttcgat cttgacggcg ttttagtcga ttccactcct
     2341 gcagtcgagc gggtttggcg aagatgggcg accgagcatg ggcttgatcc cgagtttgta
     2401 atgcagtggg cgcacgggcg ccgctccttt gagaccatcc gccgcgtcgc tccgtacatt
     2461 gatgctgcac gcgaaaacct gaaagtcgaa cgccgcgaga tcgaagatct ggaaggggtc
     2521 cgcatcatac ctggtgctgc ggaactgatc gcgtcaattc cccctggtca ctggacgatt
     2581 gtcacctctg caaccaggga gctcgcgcgc gcccgcctgg gccatgtagg actgccgctg
     2641 cctgcagagg caattaccgc cgagatggtg caaaacggga aacccgatcc cgagccgtac
     2701 ctcaagggcg caatgcaact ggcatttgcc ccaaagtcat gcatcgtgat tgaagacacc
     2761 gccgcaggta tcgcatctgc gaagagagcg ggcatgcagg cgatagcgct gaccacaact
     2821 tatccagcgc acgagctgcg ggaagccgat atcatcgtgc gttcctgcgc cgacattcgc
     2881 gtcgaagtga aatcgggcga tgacgacgat actttgctgc agattttctt cgagtacgcg
     2941 gtggaagcag acgttgtgat ttcctgaagc ttcagcttca ccaggtacac gtacgattgc
     3001 tctaacgttt gttgccacgc ccggtaccga gcgaggaatg ccgacctcat ccaaccacac
     3061 catcgttgcc catgcgtctt gccgagtgga cctcgcggga agcacgctcg atctgtggcc
     3121 gctttatttg ttccatcctg gcgcggtgac ggtgaatttc gcagttaaca tcctgactcg
     3181 ctgccaaatt actccgaacg ccgattccgg aatccacttc aaatcaatcg ataccggccg
     3241 cgaagattat tttcgcgact tcgaggagtt ctgccgggcc cgaaagattc agcatcatct
     3301 cgcggcgcac ctggtgcgtt tctttttccc tcttgggact ccgcaggatt gcggattcac
     3361 cctggaaacc aattccgagt cgcctgccgg agccggcatt tccggctcat ccgcgctcat
     3421 ggtcgcaacc gtagccgcgt tggcgcgcta caccggacgc aagctttctc gcgaacaggt
     3481 tcgcgagatc gcgcaaaacg tggaagcaca actgattagg gtaccgaccg gctgtcagga
     3541 ttattacccg gccctgtatg gcggagtgag cgctatccgt cttgatgtgg acggaatcca
     3601 tcggcaagcg atcgcggtag cgccggaaaa catcgagtcc cgcttcgtgc tggtgtacac
     3661 cggggcgccg cgacaatccg gcatcaacaa ctgggaggtg ttcaaggcgc acatcaacgg
     3721 cgaccaacgc gtcttccgca atttcaaaca gattggcgaa atcgctcgag cgatgcatgc
     3781 ggccctggag gccggcgatt ggaagcaggt gccaaagctg ctgggggaag aatggaagct
     3841 gcgccggacc aatgcgccag gaatcactac tccgctcatg gataagctca tttcggcggc
     3901 aaagcggaaa ggcgctctgg cagcgaaggt ctgcggcgcc ggtgggggcg gctgtttcgt
     3961 gctattcgtg agcgagggca gtcacgatgc tgtagccgaa gccattcgcc agcacggcgg
     4021 ccgagtcttg ccattccgag tgaatcccga tggcgtgact gtcgtggggc ggaaccgtcg
     4081 cccggctaga gaagggaacc cgctgcgcta gccacgggtg ctgtttcgct aaatgccgag
     4141 agccaaaatc gccccgcagc tagcgcagcg gccttcagat aaaccgtggg caagcgaaag
     4201 gatccatgcg ttttcataca aactgcagtt gcaattcgtc gaatggaggc gaatcatagt
     4261 gtcagtacgc gggtagttcg atggtcccgg tacactcgcg ttccgggtgt cctatgaagc
     4321 tgttacgagg ttcgcctttt cttattgctt tcgcccttgt gtcgtctctg ttcgccttcg
     4381 ata
//
LOCUS       DATNDT010000203         7303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3243823, whole genome shotgun sequence.
ACCESSION   DATNDT010000203 DATNDT010000000
VERSION     DATNDT010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3243823"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /gene="menC"
                     /locus_tag="VN788_06605"
     CDS             <1..1030
                     /gene="menC"
                     /locus_tag="VN788_06605"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HXU20234.1"
                     /translation="DLRRILLLEADVDGAIGWGESTAGENPFYSYETVETAWLILRDH
                     LWPLLKEKEIGAASEVWDLLAPVRGHNMAKAALETALWDAEAQQKNLPLAKLLGGTRD
                     EIPCGVSIGIQPTNAALITKVEEALSARYQRIKIKIKPGHDIAPVRALRERFPSVRLM
                     ADANSAYRLEDAPLLKQLDAFYLIMIEQPLGWDDIFSHAKLQRQIETPICLDECIHDV
                     EHARAAIEVQACKIINIKLGRVGGHTSARRIHDLCQENSIPVWCGGMLESGIGRAHNI
                     AMSSLPNFSLPGDVSASRRYWSEDIIEPEVEVTPQGTIRVPNVPGIGYQPRLDRIESL
                     TKRREVLE"
     gene            1032..1991
                     /locus_tag="VN788_06610"
     CDS             1032..1991
                     /locus_tag="VN788_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HXU20235.1"
                     /translation="MSLFPAPSNAPASAPPVKRPSSFLLAMGLAGVLTVWSVNYIVAK
                     LTLAHIAAFTLVPFRAELVASLLLAIYFAQPRASRTPLRARDIPTFACIGFFGFVVNQ
                     GCFVLGLSYTTSQHSVVIIALGPVVILLLASAMKLEKLTPSKILGLAISFAGVVFLET
                     EHGSPLNSPLLAGDLITLAGMSAFSLYAVLGKRVASSYDSISMNAFNAAVAAIVFMPL
                     AIHQAIKLDWKSVGWVGWIGMLYMAGVSAVVGYLLFYWLLRHMDASRVVAINYFQPVA
                     VFLLSIPILGEHLTPRLIASAALVLLGVYLAEHISRRQPSLLS"
     gene            complement(2261..2842)
                     /locus_tag="VN788_06615"
     CDS             complement(2261..2842)
                     /locus_tag="VN788_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXU20236.1"
                     /translation="MKKWLVVAGLAAALAIPSFAATSTWKIDPMHANAQFLVKHLGIS
                     TIQGDFTKISGTVMLDDSDISNSSVDVTIDVNSVDTRVQKRDDDLRSEHFFDVAKYPT
                     MTFKSTKITKEGDDKFKMTGNLTIRGVTKEVTLDGTGPTKAITAMGGLRRGVSATTKI
                     NRQDFGVAADPGMVGDEITILIDLEMTAPAGTK"
     gene            3344..4408
                     /locus_tag="VN788_06620"
     CDS             3344..4408
                     /locus_tag="VN788_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HXU20237.1"
                     /translation="MKNIRTIAFALLLFAAPAPVRAQSTVMNLPRPSQHAVLTQRIGI
                     TDITINYHRPLVNGRKVWGGLVPYGQPWRAGANENTTIAFSDPVTIDGQALAAGTYGL
                     HMIPSENEWTIAFSKMNAAWGSFTYNQAEDALRVTVKPAATDFHDALTYDFDNLKPDS
                     AVVTLRWEKLAVPFTVAVNVHQVVEDSLHKQLRGLAQYTWEGWDDSANYLLAEKTDLD
                     EALKYSNQSIQVEERYDNLMTKSQVLDALGRKDEATVARNQALDKADVNQLYGYALLL
                     QQQKKQDEAFTVYRKVAKKYPDHWRAHAAMARVYSGQGDFNSALKEANAALPGAPDPV
                     KPLLQAFIKKLQDKHDINKS"
     gene            4419..6020
                     /locus_tag="VN788_06625"
     CDS             4419..6020
                     /locus_tag="VN788_06625"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXU20238.1"
                     /translation="MKLPPGVKANDFRDALKHFEELVGKDWVFTSDEDVALYRDAYSP
                     LWGEAEDSVPSAAVAPDTVQQVQAIVRLANQFSIPLYTISTGRNLAYGGSAPVLSGSV
                     VLDLKRMNRVLEVNEKNAFALVEPGVSYFDLYRHIREKGLKLWIDCPEPGWGSPIGNA
                     IDRGAGSTPLPYRDHFDAHCGMEVVLANGEVVRTGMGALPTAQTWQQFKYGIGPLMDG
                     IFSQSNFGIVTKMGFWLYPEPEACLVGRALVQRHDDVIPFVDILANLMYAGVINTHFA
                     IRSPVFNGPPDNEKTALDTKPDGGSAEQWNRYATSKGLHFWQTDLHFYGPAKVVAAQW
                     EHVKDRLSAISGAKFEDRPIYRFPLADADIEKLAERGALGIPSLSVFSSLLSLQADVP
                     VTGHLDISPIIPVSGEALLEARRVFARTFREMGVVPQNGYAETFHWRSFIMFHGLPVT
                     HDPKVNAKTRAAYGRVVQVAAEHGWGFYRTHIAFMDKVVDTYSYNNHALQRLHETVKD
                     ALDPNGILSAGRYGIWPKHLRERKS"
     gene            6103..6819
                     /locus_tag="VN788_06630"
     CDS             6103..6819
                     /locus_tag="VN788_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20239.1"
                     /translation="MQKKTIGLLAVAFFVQFVSLNATCQAGTQPKKNPYPVMAPLNQY
                     LMPDKNSEIALARSAAPASISDGAEVMVLGRDGYTTAVKGNNGFLCIVERSWGAATDD
                     SGFWNPKVRGPICFNPVAATTFAPIFLLKTKLVLAGKSKPEIVQAIASALDKKELPAL
                     KPGAMCYMLSKQQYLGDAGKNWHPHLMFFIPGNAAKSWGADLPGSPVMASDDPEERAT
                     IFMVWVGKWSDATPAPQPMH"
     gene            complement(6847..>7303)
                     /locus_tag="VN788_06635"
     CDS             complement(6847..>7303)
                     /locus_tag="VN788_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20240.1"
                     /translation="YLEFQVRSNDADKRRLEDTLGHARELVAQDKGQEPVAFANWFPG
                     AVSVMGSARSLISFFGAPSDLSAPSSVKYGPKIKVPALVIHGDSDELALPPNAQKIYE
                     SLTASPQRDLVWVKGASHYLTPGPLAESYARLIVDWLVKNFPASPRRGS"
BASE COUNT         1528 a         2286 c         1939 g         1550 t
ORIGIN      
        1 cgatttgcgc cgcattcttc ttctcgaagc tgatgtcgac ggcgcgatcg gatggggcga
       61 atcaaccgcc ggcgaaaatc ctttttattc ctacgaaacc gtcgaaaccg cctggctgat
      121 tctgcgcgat catctttggc cgctgctgaa agaaaaggaa atcggcgctg cttcggaagt
      181 gtgggacttg ctcgcgcccg tccgcggcca caacatggcc aaagccgcgc tagaaaccgc
      241 cctgtgggac gctgaggccc aacaaaaaaa tctgcctctc gccaaacttc tcggcggcac
      301 gcgcgacgaa attccatgcg gcgtgtccat cggaatccag ccaaccaacg ccgcgctgat
      361 cacaaaagtg gaggaagctc tttcggctcg ctaccagcgc atcaaaataa aaatcaagcc
      421 gggccacgac attgcgcccg tgcgcgccct ccgcgaacgc tttccgagcg tccgtctgat
      481 ggccgatgca aattccgcct atcgcctcga agatgcgcct cttctgaagc agcttgacgc
      541 cttctacctc atcatgatcg agcagcccct cggctgggac gacatttttt ctcacgccaa
      601 actccagcgc cagatcgaaa cgcccatctg cctcgatgaa tgcattcacg atgtcgagca
      661 cgcgcgggcc gcgattgaag tccaggcctg caaaattata aatatcaaac tcggccgcgt
      721 cggcggtcac acttccgcgc gccgcattca cgatctttgt caggaaaatt ccattcccgt
      781 ctggtgcggc ggcatgctcg aatcgggcat cggacgcgcg cacaacatcg ccatgtcctc
      841 gctcccgaat ttttctcttc caggcgacgt ctccgccagc cgccgttact ggtcagaaga
      901 catcatcgag cccgaagtag aagttactcc gcagggaacc attcgcgtcc ccaacgtgcc
      961 cggaatcggc taccagccgc ggctcgaccg aatcgaatcg ctcaccaagc gccgcgaggt
     1021 tctcgaatag cttgagcctc ttccccgctc cgtccaacgc accggccagc gcgcctcccg
     1081 ttaaacgccc cagttcattt ctgctggcta tgggactcgc gggcgtcctc accgtttggt
     1141 ccgtcaatta cattgtcgcc aagctcactc tcgcgcacat cgccgcattc acgctggttc
     1201 cctttcgcgc cgagcttgtc gcctcgctat tgttggcaat ttatttcgcg cagcctcgcg
     1261 cgagtcgcac gccgttgcgt gcccgcgaca ttcccacctt cgcctgtatt gggtttttcg
     1321 gattcgtggt gaatcagggc tgctttgtcc tcggactgag ctacacaact tcgcagcatt
     1381 ccgtcgtaat cattgcgctg ggcccggttg tcattctttt gctcgccagc gccatgaagc
     1441 tggaaaagtt gacgccctcc aaaatattgg gactggcgat ttcattcgca ggcgttgtgt
     1501 tcctcgaaac cgagcacggc tccccgctga attcacctct tctcgcgggc gatctgatta
     1561 ccctcgccgg catgtccgcc ttttcgctat acgccgttct cggcaagcgc gtggcgagct
     1621 cttacgattc aatttcgatg aacgccttca acgccgcagt ggccgccatc gttttcatgc
     1681 cgctagccat ccatcaagcc atcaagctcg actggaaaag tgtcggctgg gtagggtgga
     1741 tcggaatgct gtacatggct ggggtcagcg cggtcgtcgg ctatctgctt ttctactggc
     1801 tgctgcgcca catggacgct tctcgcgtgg tcgccataaa ctatttccag ccggtcgctg
     1861 tcttcctgct ctcaatccca attctcggcg agcacctcac gccgcgcctc atcgccagcg
     1921 cggcgctggt cctgctaggg gtctatctag ccgaacacat ctcccgccgc cagccgtcct
     1981 tgctttcgta gcgccggcgc gctcgccatc tcccgtcttc gcgtcgcaca cctctgggct
     2041 tacattccgt cattccgctc gccctgacgg aggaagggag cgcagtgtcc cgctcttttg
     2101 ctcttgcgcg gagcgccggc acgcgcagtc gaggaatctc tcttcgatct caattcccag
     2161 cacgcacctt cacaaaatcc caatgtgtca gctccccaaa aaaaacggcg ccgatgaatg
     2221 acgccgcttt cgagcaaaac agcaatgtga atgtgtcagc ctacttcgtc cctgcaggag
     2281 ccgtcatctc gagatcgatc aaaatcgtta tctcgtctcc caccatgccc ggatcggcgg
     2341 ctacgccgaa atcttgccga ttgattttcg tggtcgccga aacgccgcgc cgcagcccgc
     2401 ccatcgccgt gatcgccttc gtcggaccag tcccgtcgag cgtcacttcc tttgtgacgc
     2461 ctcgaatcgt caggtttccg gtcatcttga atttatcgtc gccttccttg gtaatcttcg
     2521 ttgatttgaa ggtcatcgtc ggatacttgg ccacatcgaa gaaatgctcg cttctcaggt
     2581 cgtcatcgcg cttctgcacg cgcgtatcga cggaattcac gtcaatcgtc acgtccacgc
     2641 tcgaattact gatatccgaa tcgtccagca tcaccgttcc gctaattttg gtgaaatctc
     2701 cctgaatcgt tgaaatcccg agatgcttca ccagaaattg cgcgttggca tgcatcgggt
     2761 cgatcttcca cgtgcttgtc gccgcaaacg acgggatcgc gagagccgcc gccagtccag
     2821 ccactaccag ccacttcttc attgctgcct cctcagaatg gtttctgaaa actggaatcc
     2881 gatgcgtgcg gcgaatcgcc tgccgcccag agcttcgatc agcccaccca ccaagtttca
     2941 cgtttgggtg tctgaccggg ccgaagtata taccggtttc ccaaccggtc aacaccccga
     3001 actttcgccg ccgccctttt gagttttcgt cattccgggc ggaggtgtcc cgcgcttttg
     3061 cttttgcgcg aagcgccggg acacgcagtc gaggaatctc tcttcggtgt tcgtagggta
     3121 cacggcccgg catgactgct agcctgcgca gccagcaacc ttgttgcctc tggcgcagga
     3181 gaggcatagg atgttcccac tgcggtccaa ttggaattca gggtggatac ctttggacca
     3241 aggactccga agtgaacgcg tcaaccaatt caatttctcc ttgcgggtcg gacgccaccg
     3301 caaccgcgcg atcgcgcaat tgagttcgat gataggagcc aacatgaaaa acattcgaac
     3361 gatagccttt gcattgttgc tctttgccgc gccggctccg gtcagggcgc aatccacggt
     3421 gatgaatctt ccgcgcccca gccagcatgc ggtgctcacg caacgaatcg gcatcacgga
     3481 tatcacgatc aattatcacc gccctctcgt caacggtcgg aaggtttggg gcggcttggt
     3541 tccgtacggc cagccgtggc gcgcaggcgc gaatgagaac accacgattg cgttcagcga
     3601 tcccgtaacg atcgacgggc aggcgctagc agccggcacg tacggtttgc acatgatccc
     3661 ctcggaaaat gaatggacga tcgccttttc gaagatgaac gccgcatggg gaagcttcac
     3721 ttacaatcag gctgaagacg cgctgcgcgt taccgtaaag cccgcggcaa ccgatttcca
     3781 tgacgcattg acgtatgact ttgacaatct caagcccgat tcggccgtcg tcaccctacg
     3841 ctgggaaaag ctcgccgtgc cgttcacggt cgccgtaaac gtccaccaag tcgtggaaga
     3901 cagcctccac aagcaacttc gcggcctcgc gcaatacacc tgggaaggct gggacgactc
     3961 cgccaattat ctgctcgctg aaaaaaccga tctcgacgaa gccctgaagt attcgaatca
     4021 gtcgattcaa gtcgaagaga gatacgataa cttgatgacc aagtcgcaag ttctcgacgc
     4081 acttggacgc aaggatgaag cgacggtcgc ccgcaatcag gccctcgaca aggcggatgt
     4141 gaatcaactc tacggttacg ctctgctgct tcagcagcag aaaaagcagg acgaggcctt
     4201 caccgtctat cgaaaagtgg cgaagaaata tcccgatcac tggcgcgccc acgccgcaat
     4261 ggctcgcgtc tacagcggcc agggagattt caatagcgcg ctgaaggaag caaatgctgc
     4321 gctgcccgga gcccccgatc cggtcaagcc actcctgcag gcattcatca aaaagctgca
     4381 ggacaagcac gacatcaaca agagctgacg atccgacgat gaaactgccg ccgggagtga
     4441 aagccaatga ttttcgcgat gccctcaagc acttcgagga actagtcggt aaagactggg
     4501 tgttcacatc cgacgaggat gtggcgctct accgcgacgc ctattctccg ctgtggggtg
     4561 aagcggaaga cagtgttcca tcggctgcgg tggcgcctga cacggtccag caagttcaag
     4621 cgatcgtccg gcttgccaat cagttctcca ttcctctcta caccatctcg acgggccgca
     4681 acctcgcgta tggcggctcc gcacccgttc tttccggcag cgttgttctt gatctcaaaa
     4741 ggatgaaccg tgttctcgag gtgaacgaaa agaacgcctt cgctctggta gagcctggcg
     4801 tcagctactt cgatctctac cgccatatcc gtgaaaaggg gctaaagctc tggattgact
     4861 gccccgagcc aggctggggc agtccaatag gaaacgcgat cgaccgcggg gccggcagca
     4921 cgcctctgcc ttatcgcgac cacttcgatg cgcactgcgg gatggaagtc gtgctggcca
     4981 atggtgaggt agttcgcaca ggcatggggg ctcttccgac cgcccagacc tggcagcagt
     5041 tcaagtatgg gatcggtccc ttgatggacg gaatcttctc ccagtcaaac ttcggcattg
     5101 tcaccaagat gggcttctgg ctgtacccgg agccggaagc gtgcctggtc ggccgcgccc
     5161 tcgttcagcg tcatgacgac gtcattccct tcgtcgacat cctggccaac ttaatgtacg
     5221 ccggggtgat caacactcac ttcgcgatca gaagccccgt gttcaacggt cccccagaca
     5281 acgaaaaaac cgccttagac accaagcccg atggcggttc ggccgagcaa tggaaccgct
     5341 acgcaacctc aaaaggcctg cacttctggc aaacggatct gcacttctac ggccccgcaa
     5401 aagtggtggc cgcgcagtgg gagcatgtca aagaccgact ctcggccatc agtggcgcga
     5461 agttcgagga tcgacctatc taccgcttcc ccctcgccga cgcggacatc gagaaactcg
     5521 cggagcgagg cgcacttggc attccgagcc tgtctgtctt ctccagtctg ctttccctgc
     5581 aggcggacgt tccggtcacc ggccatctcg atatttctcc aatcataccg gtctccggcg
     5641 aagcgctcct cgaggcgcgc cgcgtcttcg cccgcacctt ccgagagatg ggcgtcgttc
     5701 cgcaaaacgg ctacgcggag acgttccatt ggcgctcgtt catcatgttc catggattgc
     5761 ccgtcactca tgatcccaag gtgaatgcca agacgcgggc cgcctacgga cgcgtggtgc
     5821 aggtagccgc cgagcatggc tggggattct accgtacgca catcgccttc atggacaagg
     5881 tcgtggacac ctattcctac aacaaccatg ccctccaacg cctgcacgaa actgtgaagg
     5941 atgccctcga tccgaatggc attctatccg ctggccgcta cggcatctgg ccaaagcacc
     6001 ttagagagcg caaatcatga gttggaaaag ggaccgtctg acaagatcga gccgcatatt
     6061 cccaaacctt aagtgaagag tgtgcgtgca cgggagagtt tcatgcagaa gaaaacgatc
     6121 ggccttctcg ctgtagcatt ctttgtgcag tttgtatcgc tcaatgcaac ctgccaagca
     6181 ggaacgcagc cgaaaaaaaa tccttatcca gtcatggctc ctctgaatca gtacttgatg
     6241 ccggacaaaa actccgaaat cgcgctggct cgcagcgctg ctccagcgtc catttcggac
     6301 ggcgccgaag taatggtgct gggacgcgat ggatacacaa ccgcagtgaa gggcaataat
     6361 ggcttcctct gcatcgtgga acgatcatgg ggcgctgcca ccgatgattc gggtttctgg
     6421 aatcccaaag tgcgcggccc catttgcttc aatcctgtag ccgcaacgac ctttgcacct
     6481 atctttctgt tgaagaccaa actggtgttg gcgggaaaat ccaaaccaga gatcgttcag
     6541 gcgatcgcat cggcactgga taaaaaggaa ctgcccgcgc tgaagcccgg agcaatgtgc
     6601 tacatgttgt cgaagcagca gtatctgggc gatgccggta aaaactggca tccgcacctg
     6661 atgtttttta ttccaggaaa cgcggcgaaa agctggggag ccgatctgcc gggctcgcca
     6721 gtgatggcat cggacgaccc tgaagagcgc gcgactattt ttatggtctg ggttggcaaa
     6781 tggtcggatg caacgccggc tccgcagcct atgcactgat ttgcgattcg aggggcgagc
     6841 acatcgctaa gaacctcggc gaggcgaggc gggaaagttt ttcaccagcc aatcgacaat
     6901 cagtctagcg taggactccg caagcggccc aggcgtcaaa tagtggctcg cacccttcac
     6961 ccagaccaag tcgcgttgcg gcgacgctgt caggctctcg taaatcttct gcgcgttcgg
     7021 cgggagggca agctcgtcgc tatcaccgtg aatcaccagg gcgggaactt tgattttcgg
     7081 cccgtacttg accgaactgg gcgcgctcag atcgctcggc gccccgaaga agctgatcag
     7141 cgaccttgcg cttcccatca ccgacacggc gcccggaaac cagttcgcaa acgcgaccgg
     7201 ttcctgacct ttgtcttggg cgaccaattc gcgcgcgtgc ccgagcgtat cctccagccg
     7261 ccgcttgtcc gcgtcgttgc tccgaacttg aaactccaga tac
//
LOCUS       DATNDT010000204         5578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1493773, whole genome shotgun sequence.
ACCESSION   DATNDT010000204 DATNDT010000000
VERSION     DATNDT010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493773"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(232..1353)
                     /locus_tag="VN788_06640"
     CDS             complement(232..1353)
                     /locus_tag="VN788_06640"
                     /inference="COORDINATES: protein motif:HMM:NF044870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-propeller fold lactonase family protein"
                     /protein_id="HXU20241.1"
                     /translation="MKLRLNYRLLFLLILLGGFAISEVALELRPSFLRPGLHLFAYVG
                     NTGDGTVTAIDLVNLSPVATISVGPSPTAVRANPSRKEIWGLSSSGGDAWVLDSPSDR
                     VVARISVGAAPSALDFSPDGARAYIVAAKSNTLIEIDCQSRTVVAKAATGREPASVRI
                     SPDGKSVLVSNRGDSTVSIFDAASLALRGVVPVAPDPGRIAILPDGSKAFIASNAKDE
                     ISVVDLKTFSLLANLSLGGAPNDLILKPDGGELYVPSGNAHGLLVVNTQTNEVGDFLL
                     LGMSPTAGVLSADGATLYATDSAAGHVVPVAIGNRQVARPISIGQSPITAELTPGGDI
                     LAVVDTTSDDLAVIRTQTSSLITLIPTGPQPRSLAIKVF"
     gene            complement(1556..1629)
                     /locus_tag="VN788_06645"
     tRNA            complement(1556..1629)
                     /locus_tag="VN788_06645"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1595..1597),aa:Cys,seq:gca)
     gene            1885..2766
                     /locus_tag="VN788_06650"
     CDS             1885..2766
                     /locus_tag="VN788_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20242.1"
                     /translation="MKKILLAAIAVSCLAGAAVSVRAQDENAASPPKVLVVTREMVKI
                     GKGAAHTKNEAAWVRAAMAAKEPDRYLAATTMTGPNAALFFEGFDSYAAWGEADKFDS
                     QPKVTALTGPMAEKDADYVSEGRQMVATLNEKWSWKPGMNIAEMRYFELETIRMRPGH
                     DREWDELIALYQGAATKMNLDEHDIFYEVHYGAPAGTVLIFTPRKSLGDLDAAMGTGK
                     AFQEALGEKGREQWGKLVEETVAADSTELLSFSPEMSYPPDEWVKSDPSYWKSKPMGG
                     KPAAAAKKGAEAPAPKE"
     gene            2903..3958
                     /locus_tag="VN788_06655"
     CDS             2903..3958
                     /locus_tag="VN788_06655"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HXU20243.1"
                     /translation="MAAENHRVELTFDPADEYRPEPLPPPEIPAWKGGGLRIGIHTSI
                     AGDIAGALEIADGLGANALQIFSSSPRMWDRGNVRISEADAARFRTRRGELGLGPLVV
                     HSNYLINLASPNRVLRTRSVQAFHQEIVRAAALGADYLVLHPGSSMGGAVESAISAIA
                     LGLRQAARGSRPGELQILLENTAGHGSSIGSKFQELRAIIDACPELNLGVCVDTAHLF
                     AMGWDIRTANGLEAALGEIESTVGLDRVRVVHVNDSKMALGTRVDRHEHIGKGKIGLE
                     AFRMILKHPQLAACAFILETPIDLPGDDKRNIAALWRLTGRVVVAKGMGMRPRRKKQS
                     SGKQMVRKQRKKRPRGK"
     gene            3960..>5578
                     /gene="leuS"
                     /locus_tag="VN788_06660"
     CDS             3960..>5578
                     /gene="leuS"
                     /locus_tag="VN788_06660"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HXU20244.1"
                     /translation="MPEYDSQRIEQKWQKIWEEQHAFDAVDGDTSRPKYYLLEMLPYP
                     SGTLHMGHMRNYTIGDAVARYKRMRGYNVLHPMGWDAFGLPAENAAIKNGTHPREWTN
                     ANIQEFRRVLRRFGFSYDWRREISTCEPEFYRWNQWFFLRMLEKGIAFRKKSKVNWCP
                     ECATVLANEQVIDGFCWRHEATLVEQKELEQWFLRITQYSDELLADMAKLQGGWPERV
                     LVMQQNWIGKSRGARVRFDVDGIAGLGLEVFTTRIDTIYGVSALLVAAGHPQMGRLLE
                     GVHGRASMDAQIKAMLQKSAKAADIATAEKEGFFTGRFALNPFSGESVPVWVANFVLA
                     EYGTGAVMCVPAHDERDFEFAEKYRLPLKLVIQPLQGVPLRADRMKEAYTEYGRLVDS
                     GPYAGLASEQAIEKMTADAATKQIGAGETTYRLKDWGISRQRYWGTPIPVVYCEKDGI
                     VPVPDADLPVRLPDNVALTGLGQSPLAGVPEWVNTKCPKCGGAARRETDTMDTFVDSS
                     WYFYRYTDPHNDRAPFDGAKVQQWFPIDQYIG"
BASE COUNT         1240 a         1408 c         1947 g          983 t
ORIGIN      
        1 ggtagaaaac ctcggcttct ccgttgagat gatccagccg ccgctccacc acttctcctg
       61 tcgcgtcatc cagcatggcc atctgctgat agcgcgcgtg aaaatcgcag cctataatca
      121 acattgctcg gctccttcct ctgagctttg gtcagttcag cactgccaag tctactcggt
      181 cgcaaggagc cgacgccgtt atccaatcaa caactgcaaa ggcgtcaatc gtcagaagac
      241 tttgatggcg agggagcggg gttgggggcc ggtggggatt agggtgatta ggctggaggt
      301 ttgggtgcgg atgacggcta ggtcgtcgga ggtggtgtcg accacggcca ggatgtcgcc
      361 gcccggagtg agttcggcgg tgatgggcga ttgtccaatg gagatggggc gtgcgacctg
      421 gcgattgcca atggcgacgg gaacgacgtg gcctgcggcc gagtccgtag cataaagggt
      481 ggcgccatcg gcggagagca cgcctgccgt gggcgacata ccgaggagga gaaagtctcc
      541 tacttcgttg gtctgggtat tgaccaccaa caggccgtga gcgttgccgg atgggacgta
      601 gagttcgccg ccatcgggtt tcaggatcaa gtcattcgga gcgccgccca gggaaagatt
      661 ggcgagcaga gagaaagttt tcagatcgac tacggagatt tcgtcttttg cattggaggc
      721 gatgaaggct ttcgagccgt cgggcaagat ggcaatccgg ccggggtcgg gagcgaccgg
      781 cacgacgccc cgaagggcta gagacgcggc gtcgaagatg gagacggtgg aatcaccgcg
      841 attcgaaacg aggactgact ttccgtccgg agagatgcga acggacgcgg gctcgcggcc
      901 ggtggccgct ttggcgacga cagtgcgcga ttggcaatcg atttcgatca gcgtgttcga
      961 tttggccgcg acgatatagg cgcgcgcgcc gtcgggtgaa aaatccagtg ccgaaggagc
     1021 cgcgccgacc gaaatgcgcg ccaccacgcg atcgcttggg gaatcgagga cccaggcgtc
     1081 gccgcccgat gaacttagtc cccaaatttc cttccgcgac gggttggcac ggacggcggt
     1141 tggcgaaggg ccgacggaaa ttgttgcgac gggggaaaga ttgaccaggt caatcgcggt
     1201 gactgttccg tcgcccgtat tgcccacgta agcgaaaaga tgaaggcccg ggcggaggaa
     1261 ggaagggcgg agctcgaggg caacttcgct gatagcgaag cctccgagaa gtatgagcaa
     1321 gaagagaagg cgatagttaa ggcggagctt cattcgagtg cagtttaagc cagatagcga
     1381 cggtgggcag ggaaaaagga gggtccacgt ggcatcaggg cggatcgagc gtcggcggaa
     1441 aatgaagaga gaggtccttc gactgcgtcc cgcgccgcgc acacaccgcg cgcaagaaaa
     1501 aggcgcggga cttcgctcag gatgacttca accccgctgc agtaacggtg gaagttggag
     1561 gcgcgaggcg gaatcgaacc gccgatgaag gttttgcaga cgtttgcctt gccacttggc
     1621 gaccgcgccc ttacgaaagc gtgggaacaa gcaatattag caaagcttac gactatgtct
     1681 ccagcggagg gcgcggagac cccgcgggcg gcaagaattg attgtgcgcg cgagcgggca
     1741 ggtgtacagt gtcgcaccag aaataggccg gatttccggc catacccgac aagccctatc
     1801 aagaaaatac aacctcgatc gcggacgcgt tccggagtgt tcgcgtacct acacaattag
     1861 ccacagccga aaaggagatt gcgtatgaag aaaattttgt tggcagcaat agcagtgagc
     1921 tgtctggcgg gtgcggcagt gtccgtgcgg gctcaagatg aaaacgccgc atcgccgccc
     1981 aaggtgctgg tggtgacccg cgagatggtg aagattggaa aaggcgcggc gcacacgaag
     2041 aacgaagcgg cgtgggtgcg cgctgccatg gcggcgaagg agcccgatcg ttatttggcg
     2101 gcgacgacga tgaccggacc gaacgcggcc cttttcttcg agggcttcga ttcctacgcg
     2161 gcttggggcg aagccgacaa gtttgacagc cagcccaaag tgacggcgtt gactggaccc
     2221 atggcggaga aggacgccga ttacgtatcg gagggaaggc agatggtggc gacgctcaat
     2281 gagaaatgga gctggaaacc gggcatgaac atcgccgaga tgcgttactt cgaattggaa
     2341 acgattcgga tgcgtccggg gcacgacagg gaatgggacg agcttattgc gttgtaccaa
     2401 ggcgccgcca cgaaaatgaa tctggacgag cacgacattt tttatgaagt ccactacggc
     2461 gcgcccgctg ggaccgtgct gatattcacg ccgcggaagt cgcttgggga tctggacgcg
     2521 gcgatgggca cggggaaagc gtttcaggag gcgctgggcg agaagggacg agaacagtgg
     2581 gggaaattgg tggaggaaac ggtcgcagct gattcgacgg aactgctgag cttcagcccg
     2641 gaaatgagct acccgccgga tgaatgggtg aagtctgatc cgagttactg gaagtccaaa
     2701 ccgatgggtg ggaagccggc cgctgcagcg aagaagggcg ccgaggcgcc ggcgccgaag
     2761 gaataaccgg ccggagaggg ccgctggaag accgaagagt tgcgcgcctg cacgcggcgc
     2821 gatggcggag gtgtgcgcct gcgttgcgcg gtggtgacgg ttcggctaaa atggtttttg
     2881 taatcccgga caacctgagg gaatggcggc agaaaaccat cgagtcgagt tgacgttcga
     2941 ccccgccgat gagtatcgcc ccgaaccgct gccgccgccc gaaattccag cttggaaagg
     3001 cggaggcctg cgaataggga ttcatacgtc catcgcaggc gatattgcgg gcgcgcttga
     3061 aatcgcggat ggattaggcg cgaatgcgct gcaaatattt tcgtcgagcc cgcggatgtg
     3121 ggatcgaggg aacgtccgaa tttctgaagc ggacgcggcg cgatttcgca cgcggcgcgg
     3181 ggagctgggg ttggggccgc tagttgtcca cagcaattat ctgatcaatt tggcatcgcc
     3241 gaatcgcgtg ctgcgaacac gctcggtgca ggcatttcat caggagattg tgagggccgc
     3301 ggcgctcggc gcggattacc tggtgctgca tccgggcagc agcatgggcg gtgctgtgga
     3361 gagcgcgatt tcggcgatcg cgttgggcct gaggcaggcg gcgcgagggt cgaggccggg
     3421 cgagctgcaa attcttcttg aaaataccgc ggggcacgga agttcgatcg gttcgaaatt
     3481 tcaagagctg agagcgatca tcgacgcatg cccggaattg aatctgggag tttgcgttga
     3541 cacagcgcat ctttttgcca tggggtggga tattcggacc gcgaacggtc tggaagccgc
     3601 gcttggcgag attgagagca ccgttgggct cgatcgcgtg cgggtggtcc acgtaaacga
     3661 ttcgaaaatg gcgctgggga cgcgggtcga ccggcacgag cacatcggaa aagggaagat
     3721 cggtctcgag gcctttcgaa tgattttgaa acatccgcaa ctcgccgctt gcgcgttcat
     3781 cctggaaacg cccatcgatc ttccgggcga tgataagaga aacatcgcgg cgttgtggag
     3841 acttaccggg cgcgtggtgg tagcgaaggg gatgggcatg aggccgcggc gcaaaaaaca
     3901 aagcagcggc aagcaaatgg tccggaagca gcgaaaaaaa cgaccacgag ggaagtgaat
     3961 tgccggaata cgattcgcag cgcatcgaac aaaagtggca gaaaatttgg gaagagcaac
     4021 acgcgttcga tgcggtcgac ggagatacat cgcgtccgaa atactacctg cttgaaatgt
     4081 tgccgtatcc ttcagggaca ttgcacatgg gccacatgcg caactacacg attggcgacg
     4141 ccgttgcgcg atacaaacgc atgcgcggat acaacgtgct gcacccgatg gggtgggacg
     4201 cgttcggcct gccggcggaa aacgcggcga tcaaaaacgg aacgcatccg cgcgaatgga
     4261 ccaacgcaaa tattcaggaa tttcggcgcg tgctgcggcg attcggattc agctacgact
     4321 ggcggcgcga gatttcgacg tgcgagccgg agttttaccg gtggaaccag tggtttttcc
     4381 tgcgcatgct ggagaaaggc atcgcctttc gcaagaagag caaagtgaac tggtgcccgg
     4441 agtgcgcgac agtgctcgcg aacgaacaag tgattgacgg attctgctgg cgtcacgagg
     4501 cgacgctggt cgaacaaaag gaactcgagc agtggttcct gcggattacg cagtactcgg
     4561 acgaactact cgcggacatg gccaagctgc aagggggatg gccggagcga gtgctggtga
     4621 tgcagcagaa ctggatcggg aaatcgcgcg gtgcgcgcgt gcggttcgac gtggacggaa
     4681 ttgcgggcct ggggctggaa gttttcacca cgcgcatcga cacgatctac ggtgtgtccg
     4741 cgctgctggt ggcggcgggg catccgcaga tgggacggct gctcgaaggc gtgcacggcc
     4801 gcgcgagcat ggacgctcag atcaaggcca tgttgcaaaa aagtgcgaag gcggcggaca
     4861 tcgcgacggc ggagaaggag ggatttttca ccgggcggtt cgcgctgaat ccattttcgg
     4921 gcgaaagcgt gccggtttgg gtcgcaaact tcgtgcttgc ggaatacgga acgggcgcag
     4981 tgatgtgcgt gccggcgcac gacgagcggg atttcgagtt tgcggagaaa taccggctgc
     5041 cgctgaaatt agtgattcag cctttgcaag gagtgccgct gcgcgccgac cggatgaagg
     5101 aggcatacac ggaatacggt cggctggtcg attcggggcc gtacgcgggg ctcgcgtccg
     5161 agcaggccat cgagaaaatg accgcagacg cggcgacgaa gcaaatcggc gccggggaaa
     5221 caacgtaccg gctgaaggac tggggaattt cgcggcagcg ctactggggc acgccgattc
     5281 ccgtggtgta ctgcgaaaag gacggcatcg taccagtgcc ggatgccgat ttgccggtgc
     5341 gcctgccgga taacgtggcg cttaccgggc tgggacagtc gccgctggcg ggcgtaccag
     5401 aatgggtgaa tacgaagtgc ccgaaatgcg gaggagcagc gcggcgagag acggacacga
     5461 tggatacgtt tgtcgattcg tcgtggtatt tttaccgcta tacggacccg cacaacgatc
     5521 gcgcgccgtt cgacggcgcc aaggtgcaac agtggtttcc gatcgaccag tacatcgg
//
LOCUS       DATNDT010000205         9667 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1744565, whole genome shotgun sequence.
ACCESSION   DATNDT010000205 DATNDT010000000
VERSION     DATNDT010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9667
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1744565"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="VN788_06665"
     CDS             <1..632
                     /locus_tag="VN788_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20245.1"
                     /translation="YAQYVNIIVKGTVPSGTDTSGVFGPPNTNLAGQSFTVMFLFNRG
                     LGTQYDNGGDCVKTICVSSVGGNGAQSPGIAYLTIGSGVPYLLDVNGSPQGSSFSQRT
                     VSPDAPFPSAYFETAQVYTPFGSAEVILQRTVNHPLTIPGGWSTPFSDSGADNTSGAA
                     ISGAALAHGDDRFLGLALFHRRSWRRKVGAAGLELRARVDGTIRARHCG"
     gene            568..804
                     /locus_tag="VN788_06670"
     CDS             568..804
                     /locus_tag="VN788_06670"
                     /inference="COORDINATES: protein motif:HMM:NF025930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20246.1"
                     /translation="MRLGLNYVRGLTEQSARAIVDERSRRPFACIQDLGHCGRMRRRW
                     PMLKTASLKSAGRPDCGWPRRSFLRLNRWPEWSE"
     gene            complement(1056..2321)
                     /locus_tag="VN788_06675"
     CDS             complement(1056..2321)
                     /locus_tag="VN788_06675"
                     /inference="COORDINATES: protein motif:HMM:NF024627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HXU20247.1"
                     /translation="MPWATVGRAMNMCYGPGDRVFFRAGDTFKGRFYLNLGCTLEGTP
                     TAPITIATYGAGRAAIDGGLEVENKAGITLQNLLLFSSSPSNTLDGVAFYGTTHDYRQ
                     RRYIHIDRVEVKQFGGFGIVIGSWWADSGGYSDVRITNSVSHDNRKGGIVTYGSATGA
                     LYVNKNIYIGHSTAYNNTGYTLANFPAGWRAQGTGNGILLGSVDGALLEYSAAYNNGA
                     ENDYIDGPVGIMAYDSRGVVIQYCASYANKTRAHDGDGFSLDRNTSNSILRNNWSHDN
                     YGVGLYLAQNVDQPYHFGNTVAYNLSQNDGRRGAYAGISLWGPMHGAQIYNNRVVVGP
                     TSSGSPTAFSLSNAYLAGDYMQNISVHDNVFQTTGNVPLIYADPDLLAGSAGISLANN
                     WFFAQNFRVIWGAAEYVSSADWTSATGMR"
     gene            complement(2555..2734)
                     /locus_tag="VN788_06680"
     CDS             complement(2555..2734)
                     /locus_tag="VN788_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20248.1"
                     /translation="MEFAALSDIIPMRDGENVLPYRTSPNPLPPNQELVALASSVTPD
                     YLNVMGYSVACRPLL"
     gene            complement(2927..4369)
                     /locus_tag="VN788_06685"
     CDS             complement(2927..4369)
                     /locus_tag="VN788_06685"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU20249.1"
                     /translation="MPHLRRKYDKDTCAMLACLFALLLAGCSRTVKSELAEAARAYND
                     GNLSRAIELYGRITHKHKDSGEAFYGLGISRLADHDLAGAEVALTHATGLLKGTGEQT
                     EAYIQLSDVYLQLHRSDRMYMDAAQRMADLVLARDPNSFDGHRLWAVLAHVRGAELSK
                     TDPATARAWYEKSISESRRADSARPNQPAVLSGLAAALEETGRAAEAEQAYWKLVGIE
                     PAASDPYFHLLTIEQQRGETAEAVTTARSAIESHPADFDFLISMANHGLRLDREDLFE
                     LFANRMASVAGRSKLTRLRLGRLYESAGMMDKALEQYRLGESGEASERLACREAAVSL
                     LIKVHRLPEAEAGLKKLEQGEAASEDVKVLRASLLTEQNMAAKAVPLLEMLLSHNPKN
                     VKATYQLARAYRQMGREEMAAYMFDRCDHLSPGFTPALVERADIYLSRGDRIWAKSLA
                     ARVLNVEPANPEARRIEHACLQDGDGKRSH"
     gene            complement(4380..6107)
                     /locus_tag="VN788_06690"
     CDS             complement(4380..6107)
                     /locus_tag="VN788_06690"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXU20250.1"
                     /translation="MSEGLAGLPKAGNVKPAPRGLSARGVLDLIQHMADGFGALFDEL
                     SNSPWIVIPAFTLIYLAATLKIASAKLLWDDEFFTLYISRDVHGILGALATGADQHPP
                     SFYYLTHLITNILGTNHITFRLPAVVGYWLMCVCLYFIVGRLLSRAWGAVAMLIPLAS
                     PLYYYASEGRGYGLMVGLSALAWLIWLRACEGGNRKVLLPALAMCLAGAVSSHYYAIL
                     ILLPLGLGELTRWALLKKVDFPMALAFCAPAVPLLLFLPIIRKASGYSKHFWARPHWS
                     SLYYFYPSNLGPLQDIAIVAVIVALCYRVFFGHSAGAADPARKQRPWELASVIGLVAI
                     PLAAMAIAKLVTNGFSDRYALTGLIGVAVVLCYLMFYISRGVTVIALIVWTGCLLSFG
                     FNGNVLLSKGRTVRSDVAGEIHLLESTGKTPIAVAEVTVFHRLSFYAPRELVSRLTYV
                     ADPKESIKYLGHDTVDRGLLALRPWFPIHVVPLSIYLARTPKFLVYGYVGEWTWLTYD
                     LSRPGLHTTLVRRRKDRILLSVQNDNANEIPAPPDQGESGSLFAKLPPTGEPICDMYM
                     ESIHCPAFR"
     gene            complement(6104..7666)
                     /locus_tag="VN788_06695"
     CDS             complement(6104..7666)
                     /locus_tag="VN788_06695"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional class I SAM-dependent
                     methyltransferase/glycosyltransferase family 2 protein"
                     /protein_id="HXU20251.1"
                     /translation="MRDFRSLGESLRSLEMAREELWRKQARSARTKLRWRALMVKHCF
                     HILPGETVLELGGGSGMWSEQLNSVLRSENPLTVAVFTPELLAKARERQLPNTSFVRV
                     ESILDDLPHGEYDYLVGFAALAREHMKEELAAVYRLLKPGGQILFFEANSWNYWLKLS
                     PRFRRMQRAGGGFPVSRPKMLHACSHLGYTHIELAPYDLVPASLPDGIITRVQAKTVL
                     FEHAPVLRGLCWSAYLWARKPDNGASRRPTPNLAEHAALFGKISVVVPCHNEASNIAE
                     LVERLLQLYGPYIREIIIVNDNSSDGTAEVAEKSMALESRIRLINRGKPNGVGRALRD
                     GYKAATGAYILSMDCDFVEILPELRALFDVIAAGHDGAIGSRFSHESTLVNYPFSKLL
                     FNRVFHAVVKMTLLRTVRDVTNNLKLYRAEILKELDIESAHFSANLETGLKPMLAGYD
                     IKEVPISWINRTIGMGNSNFDARSVGMDYLRALFQIWRAWRFPKKHVIPVAPGPAAPA
                     VPGTSRSRAAGK"
     gene            7887..8963
                     /locus_tag="VN788_06700"
     CDS             7887..8963
                     /locus_tag="VN788_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXU20252.1"
                     /translation="MRALVTGSGGLIGSECVRMLGEQGWEVAGIDNNMRQIFFGEGGS
                     TLGNTEALKRGVKRFRHYDVDIRNRQDVRDLIERERPQFIIHTAAQPSHDKAASIPYL
                     DFDVNAGGTMNILVAARDFCPESPICFTSTNKVYGDRPNELPLVEKEERYDYADGRDG
                     IDETMSIDQCLHSVFGASKAAADLMCQEFGRYFKMPVGVFRGGCLTGPQHAAVELHGY
                     LAYIVMCAVTGKLYTLYGYKGKQVRDQIHCRDVASLFLEFLRNPRFGEVYNLGGGRRN
                     SLSVLETVRLLGDMGYKLDYRYDDRNRVGDHICYISDLTKIRNHFPAWKLEYGLPRIL
                     EEIVERRVNSAAAANAHARVRPSF"
     gene            9288..>9667
                     /locus_tag="VN788_06705"
     CDS             9288..>9667
                     /locus_tag="VN788_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20253.1"
                     /translation="MRGKVISDPPLLTNSFHVNIGIQETMKSIHSDIFNRVRKFRFVP
                     VLVCLGVSWAQGLVQNGTFEGYPAIVLSNSKLELTVMKKGSTLANLTLRDDSEKLSPF
                     WNPMRMARELGRPVKFDGGAGLFV"
BASE COUNT         2067 a         2802 c         2761 g         2037 t
ORIGIN      
        1 tctacgccca gtacgtgaac attatcgtca aaggcaccgt gccctcgggc acggacacgt
       61 cgggagtgtt cgggcctccg aacaccaatc tggccggtca atcgttcacc gtgatgtttc
      121 ttttcaaccg cggcctgggc actcagtacg acaacggcgg cgattgcgtc aagacgatct
      181 gcgtttccag cgtcggcggt aatggcgcgc agtcgccggg gatcgcttat ctgaccatcg
      241 ggtcgggcgt gccgtacttg ctcgacgtca atggcagtcc ccaagggagt tcgttctccc
      301 agcgcaccgt ttcgcccgat gcgccttttc cgtccgccta ttttgagact gcgcaagtgt
      361 acactccctt cggaagcgcc gaagtgatac tccagagaac cgtaaatcat cccctcacca
      421 ttcccggcgg atggagcacc ccgttctccg actcgggtgc cgataacacc agcggagccg
      481 ctatttcagg agcagctctt gcgcatggcg atgatcgttt cctcggattg gctctgttcc
      541 atcgaagatc atggcggcga aaagtgggtg cggctgggct tgaattacgt gcgcgggttg
      601 acggaacaat ccgcgcgcgc cattgtggat gagcgcagcc ggcgtccgtt cgcgtgcatt
      661 caggatctag gacattgcgg gagaatgcgc aggcggtggc cgatgctaaa gaccgcctca
      721 ctgaaatcgg caggacgacc ggactgtggc tggccgagaa gaagcttcct ccgattgaac
      781 agatggcccg agtggtcgga atgaaagcgc gatatacgac tatttctatc ggataacctc
      841 agatacggtt catttcaatc cgagggtcgc gttgcgaaac ggctggggcg aaaacccgcg
      901 gagcagcagg ttcggcatga agaacttctg caggtattac ctatgtttca gccagattta
      961 cagcattttc cttttcaacg ctttcagtaa gtcctttaag gcggagctta accttgccga
     1021 gttggactgg cgatcgccgg caccaggctg gacggtcagc gcataccagt ggcactggtc
     1081 cagtccgcgg acgagacata ttcagcggca ccccagatga ctcgaaagtt ttgtgcgaaa
     1141 aaccagttat ttgcaagaga gataccggcg gagcccgcca gcagatcggg atcggcgtaa
     1201 attaacggca cgttgccggt agtctggaat acattgtcgt gcaccgagat attctgcatg
     1261 taatccccgg ccagataggc gttgctcaac gaaaacgctg tcggcgagcc actcgacgtc
     1321 ggaccgacta cgacacgatt gttatagatc tgtgcgccgt gcattggtcc ccagaggcta
     1381 atgccagcgt atgcgccgcg tcgtccgtcg ttctggctca ggttgtaggc cactgtgttg
     1441 ccgaagtggt agggctggtc gacgttctgc gccaggtaca agccgacgcc gtagttgtcg
     1501 tgcgaccagt tgttacgcag gatggaattc gacgtgtttc gatcgagcga aaaaccatcg
     1561 ccgtcgtgtg cgcgtgtttt gttggcgtac gatgcgcagt actgaatcac aacgccgcgg
     1621 gaatcgtatg ccatgattcc gacaggtccg tcaatgtagt cgttttcggc gccgttgttg
     1681 tacgccgcgg aatactctaa caatgctcca tcgacgctac cgaggaggat gccattgcca
     1741 gtgccttggg ccctccaccc cgcagggaag ttagccagcg tgtatccggt gttgttgtac
     1801 gccgttgagt gtccgatata gatgttttta tttacgtaaa gcgcgccggt ggcgctcccg
     1861 tatgtgacga tgccaccctt gcgattgtcg tgactgacgg agttcgtgat tctgacatcg
     1921 gagtacccgc cggagtcggc ccaccagctg ccgataacga ttccgaagcc gccgaattgt
     1981 tttacttcaa cgcggtcaat atgtatatat cgccgctgcc gataatcgtg agtggttccg
     2041 taaaatgcca cgccatccag ggtattggaa ggagaactgc taaaaagtag aagattctgc
     2101 agggtaattc cggccttatt ttcgacttcc aatccgccat caatcgccgc tctgcccgcg
     2161 ccatacgtgg cgatggttat gggagctgtc ggcgtgcctt ccagcgtgca tcctaaattc
     2221 aaatagaatc ggcccttaaa ggtgtcgccg gcacggaaaa acacccggtc tcccggcccg
     2281 taacacatgt tcatcgcgcg gccgaccgtt gcccacggca tgccaatggt tcccggatac
     2341 gtatcagatc cgttttgcgc aacataataa tcggcgccga atgcaagtgt agttacagcg
     2401 aacaatatgc aatatttcat gctgaattta actggtggtg ggaacgggta tatgttctca
     2461 gcggcagggc acagtctcgc gaacaaattg cgatgcgaag cacggggcgc gtcgattgac
     2521 taccaccggt tcactgccct ctcgatcgtg ctcattaaag aagcggcctg caagcaacgg
     2581 aatagcccat gacgttcagg tagtcgggag ttacgctgga ggcgagcgcg accaactcct
     2641 ggttgggagg cagcggattg gggctggtcc ggtatggcag gacgttttcc ccgtcgcgca
     2701 tagggatgat atctgaaagg gcggcgaatt ccacttcggg cagatgccgc gtgcgatcga
     2761 gtacgtcccg ccaggctggc gtatctggga tgagttatcg cgccttcggg gaaattgcga
     2821 atcgggcggt cagtacgttc tgtgcgttga agccgggatc aagagaagac agtgtcaaca
     2881 gagtgcggcc gagcacgggt gcggaagcaa cctcggcggc cccggattaa tgcgacctct
     2941 ttccatcgcc gtcctgcagg caggcatgct cgattcttcg cgcttccggg tttgccggtt
     3001 ccacattgag tacgcgggcg gcaagactct tcgcccaaat gcgatctcct cgcgaaaggt
     3061 agatgtccgc gcgttcgaca agtgcgggag tgaagccggg cgacaggtga tcgcaccggt
     3121 cgaacatgta ggccgccatt tcttcccgcc ccatctggcg atacgctctg gccagttgat
     3181 aggtcgcttt gacgtttttg gggttgtggg acaggagcat ctccagcagc ggtactgcct
     3241 tggctgccat attctgctcc gtcaatagcg atgcgcgcaa cactttgaca tcctcggacg
     3301 ctgcctcgcc ctgttccagt ttcttgaggc ctgcctcggc ctccggcagg cggtgaacct
     3361 tgattagcaa gctcaccgcc gcctccctac acgcgagccg ctccgatgcc tcaccgcttt
     3421 cccccagccg gtactgttcc agcgccttgt ccatcatgcc cgctgactca tacagccgcc
     3481 cgagcctcag cctcgtcagc ttcgatctcc ccgcaacgga agccatccga ttcgcgaaca
     3541 gctcgaacag atcctcgcgg tcgagccgta aaccgtgatt cgccatggaa atcaggaaat
     3601 cgaaatcggc cggatggctc tcgatcgcgc tccgggccgt cgtcaccgcc tcggcagttt
     3661 caccgcgctg ttgctcgatc gtcaggagat gaaagtatgg atcggatgcc gctggctcga
     3721 tccccacgag cttccaatat gcctgctccg cctccgctgc cctgccggtc tcctccaggg
     3781 ccgccgcaag tcccgataac accgccggtt gattcggccg cgctgaatcg gctctccgcg
     3841 actccgaaat tgacttctca taccaggctc gcgccgtagc cgggtcggtt ttcgaaagct
     3901 ctgcgccgcg cacgtgcgcc agcacggccc ataagcggtg gccgtcgaag ctgttcggat
     3961 cgcgcgccag cacaagatcc gccattcttt gggcggcatc catatacata cggtccgatc
     4021 tgtgaagttg aaggtacaca tccgaaagct ggatataggc ctccgtttgt tccccggtcc
     4081 ctttaagcaa gccggtcgcg tgagtgagcg ctacttcggc gcccgctagg tcatggtccg
     4141 ccagtctaga tatgcctaag ccatagaagg cctcgcccga atccttgtgt ttgtgtgtga
     4201 tccggccgta aagctcgatc gcgcggctca gattgccgtc gttataagcc cgcgcggcct
     4261 cggcaagctc gcttttgacc gtcctggagc agccggccag aagcagcgcg aacaagcacg
     4321 caagcatcgc gcatgtatcc ttgtcgtatt ttcgtcggag atgaggcacg gcagtcagct
     4381 catcggaagg ccgggcagtg gatactttcc atatacatat cgcaaatcgg ctcgcccgtt
     4441 gggggcaatt tcgcaaacag actcccgctc tcgccttggt cgggtggcgc cggaatctcg
     4501 ttggcgttat cgttctgtac cgagagtaaa atgcggtcct tgcgccgtcg gacaagcgtc
     4561 gtgtgcaaac ccggtcttga cagatcataa gtgagccagg tccattcgcc cacgtaaccg
     4621 tagaccagaa attttggcgt ccgcgcgaga tagatgctca gcggaaccac gtggatcgga
     4681 aaccacggcc gcagagccag aagaccacgg tcgaccgtat cgtgccccag atactttatc
     4741 gactctttcg ggtccgccac gtaggtcagt cgcgagacga gctctctcgg cgcatagaaa
     4801 gagagtcgat gaaacacggt tacttccgcg accgctatcg gtgtcttgcc tgtagattcg
     4861 agtaagtgta tctcacccgc aacgtccgat cttacggtcc gacccttgga tagtaataca
     4921 ttaccattga aaccaaaact aagcaggcat ccggtccaga caatcagggc gataacggtt
     4981 actccgcggg agatgtagaa catcagatag cacaacacga ctgcgacacc gatgagtccg
     5041 gtcagagcat agcggtccga aaatccatta gtaaccagct tggcgattgc catcgccgcg
     5101 agagggatcg ccacaagccc gatcacggat gccagttccc agggccgctg tttccgcgcc
     5161 gggtcagccg cgccggcgga gtgcccgaag aatacgcgat agcacagggc cacgatcacc
     5221 gccacgatcg cgatatcctg cagcgggcca agattggacg ggtaaaagta atacagactg
     5281 gaccagtgcg gccgggccca gaagtgcttg gaatagccgc tcgctttccg gatgatcggc
     5341 agaaacaaca gtagcggcac tgccggagcg caaaatgcca gcgccatcgg aaaatccacc
     5401 tttttcagta acgcccatcg ggtgagttct cccaaaccga ggggcaacag aatcaggatg
     5461 gcgtagtaat ggctcgacac agcgcccgcc aggcacatcg ccagcgcagg cagcagcacc
     5521 ttgcggttgc cgccctcaca cgcccgcagc cagataagcc acgcgagcgc cgacaagccg
     5581 accatcaggc cgtagccgcg cccttccgaa gcatagtagt aaagcgggct ggcaagcgga
     5641 atcagcatcg cgaccgcgcc ccatgcccgg ctcagcagac ggcccacgat gaaatacaaa
     5701 cagacacaca tgagccaata acccaccacc gccggcagcc tgaatgtgat atggtttgtc
     5761 cccagaatgt tagtaatcag atgggtaagg tagtagaaac tgggtgggtg ctggtcggcg
     5821 ccggtggcca gagccccaag aatcccgtgt acgtccctcg atatatacag cgtaaaaaac
     5881 tcgtcgtccc acagcagctt ggcggacgcg attttcaaag tcgcggccag atagatgagc
     5941 gtgaatgcgg gaattacgat ccacggcgag ttgctaagct catcgaacag cgccccgaac
     6001 ccatccgcca tgtgctgaat caggtctaga accccgcggg cgctcaagcc gcgcggggcc
     6061 ggtttcacgt tgcctgcttt cggcaagccg gcaagtccct cgctcatttg ccggcagctc
     6121 gcgatcggga ggttccggga acagcggggg cggccggccc cggcgccacg ggtatcacgt
     6181 gtttcttcgg aaaccgccac gcccgccaga tctggaaaag cgcgcgcaaa tagtccatcc
     6241 caaccgaacg cgcgtcaaaa ttcgagttcc ccatgccgat cgtccggttg atccacgaga
     6301 tcggtacttc tttgatgtcg tatccggcaa gcatcggttt caggccggtc tcgagattcg
     6361 cggaaaaatg cgccgactcg atgtccagct ctttcagaat ctcagcgcgg tacagtttca
     6421 gattgtttgt aacgtcccgg actgtcctta gcagggtcat tttcacgacc gcgtggaaaa
     6481 cgcgattgaa caacagtttt gaaaacggat agttcaccag cgtcgattca tgggaaaagc
     6541 ggcttccgat tgcaccgtcg tgcccggccg cgatcacatc gaagagcgcc cgcaactccg
     6601 gcagaatctc gacaaaatcg caatccatcg acaatatgta ggctcccgtc gcggccttgt
     6661 aaccgtcccg caaggcccgc cccacgccat tcggtttgcc gcggttgatc agccggatcc
     6721 ggctttccaa tgccatcgat ttttccgcaa cctccgcggt cccatccgag ctgttgtcgt
     6781 tgacgatgat gatctcgcga atgtacggcc cgtaaagttg aagcaggcgc tccaccaatt
     6841 ccgctatgtt cgacgcctca ttgtggcatg gcacgaccac cgatattttc ccgaagagcg
     6901 ccgcatgttc ggcaaggttt ggggtgggcc tccgcgaagc gccgttgtcc ggtttgcgag
     6961 cccacaggta agcgctccag catagcccgc gcagcaccgg cgcgtgctca aaaagcaccg
     7021 tcttcgcctg cacgcgcgta atgatcccgt ccggtaggct tgccggtact aaatcgtatg
     7081 gagctagttc gatatgcgta tatcccagat gcgagcacgc gtgcagcatt ttcggccgcg
     7141 ataccggaaa gccgccaccc gcccgctgca tccggcggaa gcggggactc aatttcagcc
     7201 aataattcca cgaattcgct tcaaagaaca ggatctggcc gcccggtttc agtagccggt
     7261 aaaccgcggc aagttcctct ttcatgtgct cccgcgccag cgcggcgaag cccacgaggt
     7321 aatcgtactc cccgtgcggc aggtcgtcga gaatgctctc gaccctcacg aacgatgtgt
     7381 tgggaagctg ccgctcccgc gccttcgcca atagttccgg tgtaaagacc gcaactgtga
     7441 gagggttctc agaacgcagc acggaattca attgctcgct ccacattccg ctgccccctc
     7501 ccaattcgag caccgtttca cccggaagaa tatgaaagca gtgcttaacc atcagagcgc
     7561 gccagcgaag ctttgtgcgg gcgctccgcg cctgtttgcg ccagagctcc tcgcgcgcca
     7621 tctccaagga tcgaaggctt tcgcccagtg aacggaaatc tcgcattcaa atcaagcctt
     7681 atgactaatg gttccacgcc ttcgttacat ttcaaataat ttctggctag taggagatgc
     7741 tggaactggc ggtactattt cgcgctttga agtttctcta cgctaggttg ccttgagctt
     7801 agcgctcgcg agtagaaatc aattgcgcgt tcctccgcgc cgccagcacg ggtgctgtga
     7861 gatgaaaggc ggttaaatag aaatgtatga gagctcttgt aaccgggtcg ggcggattga
     7921 tcggttccga atgcgtgcgg atgctgggag agcagggctg ggaagttgct gggatcgaca
     7981 acaacatgcg ccaaatattt ttcggcgaag gcggatcgac gctggggaac accgaggcgc
     8041 tcaaacgcgg agttaagcgg ttccgccact atgacgtgga cattcggaac cggcaggacg
     8101 tgcgcgatct aatcgagcgg gagcgtccgc aattcatcat tcacacggct gcgcagccct
     8161 cccatgacaa ggcggcgtcg attccatacc tggattttga cgtgaatgca ggcggcacga
     8221 tgaatattct ggtggcggcg cgcgacttct gtccggagtc tcccatctgc tttacgagca
     8281 cgaacaaagt gtatggcgac cgtccgaatg agttgccgct agttgaaaag gaggagcgct
     8341 acgactacgc ggacgggcgc gacgggatcg acgaaacgat gtcgatcgac cagtgtcttc
     8401 attctgtgtt tggcgcgtcg aaggccgcgg cggatctgat gtgccaggaa ttcggccgct
     8461 atttcaagat gccggtgggc gtgttccggg gcggatgcct gaccgggccg caacacgcgg
     8521 cagtcgagtt acacggctat ctcgcttaca tcgtgatgtg cgcggtgacg ggcaagctgt
     8581 acacccttta tggatacaaa gggaagcaag tgcgtgatca gatccattgc cgtgacgtgg
     8641 cgagtctctt cctggagttc ctccgcaacc cgcgtttcgg ggaagtctat aatctaggcg
     8701 gcggccgtcg gaatagtttg tcggtccttg aaactgtccg gctgctcggc gatatgggat
     8761 acaagctgga ctaccgatat gacgacagga accgagtcgg cgatcatatc tgctacattt
     8821 cggaccttac gaagatccga aatcatttcc ccgcctggaa acttgaatac ggtttgccgc
     8881 gtatactcga agaaattgtg gagcgtcggg taaattctgc cgctgctgcc aacgcccacg
     8941 ccagggtacg cccttcgttc tgaaaaccac accagatatc cgatcgtctc gccgagccaa
     9001 cgagtaatcc cagccgtccg gcctgagtag tgaacgatct cgctttcacg gaatgtctcg
     9061 acgccgccgg gctggtgccg cgcgattccg cggaactgcg gaaacctaag gatgggggcg
     9121 cgatagtgat tgcaatcacc caaacaccat gccgggagta acgccatctc cggtgagaac
     9181 tcgcgcgggt cgattgtcgc gccagttggc ctccctgcgg aatgatctgc gaataaagac
     9241 cctttttcgc gacctacaac tgatgccgtc ggtgaaggcg gtaaaccatg cgcggaaagg
     9301 tgatttctga tccgccgcta ttaacgaatt catttcatgt gaacatagga atccaggaaa
     9361 caatgaaatc aatccactct gatatcttca accgcgtgcg taaattcagg tttgttcctg
     9421 tacttgtttg cctcggggta agctgggcgc aaggattggt ccaaaacgga accttcgaag
     9481 gttatcccgc tattgttctc tcaaactcca agctggaatt gacggtcatg aagaagggca
     9541 gcacgctggc gaatctgaca ctaagggatg acagtgagaa gctcagcccc ttttggaatc
     9601 cgatgcgcat ggcgcgcgaa ctcggacgcc cggtaaagtt tgacggcgga gctggacttt
     9661 ttgtttg
//
LOCUS       DATNDT010000206         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_879665, whole genome shotgun sequence.
ACCESSION   DATNDT010000206 DATNDT010000000
VERSION     DATNDT010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879665"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VN788_06710"
     CDS             <1..294
                     /locus_tag="VN788_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20254.1"
                     /translation="KDVYLQTTESMQADYTMVIPFLVKALLDNRKRYEARLDDVGEEK
                     LFASEPRARGYLRPRDGYRLFERREPLRARLLEDVRANAAWLRESIDYSLPTV"
     gene            352..1557
                     /locus_tag="VN788_06715"
     CDS             352..1557
                     /locus_tag="VN788_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXU20255.1"
                     /translation="MTSKRTGHSGTPLLSLRTKPLPPHPDPTAQRFGKTRRPFGDPVA
                     IAKNTPYFIPPPPTNLVNLNMPMSAILPDEASVSETAGKLVFHSVGDTGGIHGDDVEI
                     AISNAMEAQIDTTKTDGSQPAFLYLLGDVIYYNGVSTLYNAQFYEPYKYYPRAIFAIP
                     GNHDGDIRTRANDPVDHEPSLTGFMQNFCDTAPHNIYQYRTTMTQPYVYWTFDTPLAT
                     IIGLYSNVEGSLDARSTNEQQTWFEQQMKNASPDKALIVTVHHPPYSLDASHGGTPDI
                     GIAIDRAIQAASGRAPDAVLSGHVHNYQRFERTLGGKKIPYIVAGAGGYADTATLLHK
                     LQSGTTNLKLPFQTTLPDVKLMSFNESEPGFLRVTVDSTTLTFEYYTVPFGGAAVSRF
                     DSIQVKWKS"
     gene            1700..2536
                     /locus_tag="VN788_06720"
     CDS             1700..2536
                     /locus_tag="VN788_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20256.1"
                     /translation="MALVRRAGQFVIASVITLPLALAITRPNPKEIIRRSVAANEANW
                     KIAPHLVFTDHDVETKRGGNRSDKTVRVYMIDGSPYKKLLAVNGQPLSAQQEAAEKAK
                     MQHEIRRRGRESASARASRVAEYQKERTRDHAMMKEMGQAFVYKLRGEQKLNGHDVYV
                     MDATPKPGYQPKTRETKVLTGMKGTLWIDKETYQWVKVEAEVVQPVNFFGFIAKVGPG
                     TRFMLQQEPVGPDQNWMPTRFSMKVNATVLGIFNEDSTDDETFTDYQPMNKSNLTAEL
                     SE"
     gene            complement(2545..>3202)
                     /locus_tag="VN788_06725"
     CDS             complement(2545..>3202)
                     /locus_tag="VN788_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HXU20257.1"
                     /translation="SCEICPRLVAYRAEVARTKRRAYMDWTYWGKPVPGFGDPAAELL
                     IVGLAPAAHGANRTGRMFTGDRSGDFLYRALHETGFASQSFSTHIGDGLKLQNAYVTA
                     SVRCAPPGNKPTPEEFRACRPWLSQEIDLLPRVRCVVALGRIAFDSYLTILQERGLIA
                     RRSAFTFGHNKKHRIDPHLPVLITSYHPSQQNTSTGVLTFEMLRDVFRAAREELEDFR
                     "
BASE COUNT          770 a          902 c          938 g          592 t
ORIGIN      
        1 aaggatgtct acctacagac caccgagagc atgcaggcgg attacacgat ggtgattccg
       61 ttcctggtga aggctctgct cgataatcgg aagcgctacg aagcgcgtct ggacgatgtt
      121 ggcgaagaga aattattcgc cagcgagccg cgggcgcgcg gttatctgcg cccacgggac
      181 gggtaccggc tgtttgaacg tcgcgagccg cttcgggcac ggctcctcga agatgttcgc
      241 gctaatgctg cctggctccg cgaaagcatc gattattctc tgccaacggt gtaacgcaga
      301 gacgcgccgc gacagtttcc accgatcggg aaactccaaa aggaggtcca catgaccagc
      361 aagcgcaccg ggcactccgg taccccgctc ctcagcctga ggacgaaacc tctaccgccg
      421 catccggacc ccaccgccca gcgattcggc aaaacgcgcc ggcccttcgg agatccggtt
      481 gcgattgcca aaaatacccc atactttata ccgcctccgc cgacgaatct tgtgaacctg
      541 aatatgccga tgagcgcgat cctgccagat gaggcgagcg tatcggagac cgccggaaag
      601 cttgtcttcc attccgtggg cgataccggc ggcattcacg gcgacgacgt cgaaatcgcg
      661 atctcaaacg ccatggaagc ccagatcgat acgacgaaaa ccgatggcag ccagccggca
      721 tttctctatc tgctgggcga tgtcatctat tacaacggcg tcagcacgct ttacaacgcc
      781 cagttttacg agccctataa atattacccg cgggcgatct tcgcgattcc cggcaatcac
      841 gacggcgaca tccgtacccg cgcgaacgac ccggtggatc acgagccgtc gctgaccggt
      901 ttcatgcaga atttttgtga cacggctccc cacaatatct accagtaccg gaccacaatg
      961 acgcagccat atgtgtattg gacattcgat acgccgctcg caaccatcat cggactgtat
     1021 tcgaacgtgg agggcagttt ggatgcgcgc agcacgaatg agcagcaaac gtggttcgaa
     1081 cagcagatga aaaacgcctc tccggacaag gctctgatcg tgacggtaca ccacccgccg
     1141 tattcactcg acgcgagcca cggagggact ccggatattg gaatcgcgat cgatcgcgcg
     1201 atacaggccg cgagcggacg tgcgccggat gccgtactgt ccggccacgt ccacaattac
     1261 cagcggttcg agcgcacgct tggaggcaag aagattccct atattgtggc tggggcgggc
     1321 ggatatgcgg acacggccac gctcttgcac aagttgcaga gcggcacaac gaacctgaag
     1381 ctgcccttcc agacgacact gcccgacgta aagctgatga gctttaacga gagcgagccg
     1441 ggatttctgc gggtgaccgt ggactcgaca acgctgacgt tcgagtatta cacggtacct
     1501 ttcggcggag cggcggtaag ccgctttgat tcgatccaag tgaagtggaa gtcttaagac
     1561 caaccgggca agaattaccc gttttataca ttacctaccc tagggcaaga gccatctcac
     1621 ggtgacgcct caatgatttg cgatatgatg tgcgctttgg cagtctgttt gctgccttaa
     1681 ataacggaag gggaatcgta tggctttagt aaggcgggct ggacaatttg tgatcgcctc
     1741 cgtgatcacg ctgcctttag cgctcgccat tacgcgcccg aacccgaaag aaattattcg
     1801 ccggtcggtc gccgcaaacg aggcgaactg gaagatcgca cctcatttgg tatttaccga
     1861 ccacgatgtg gaaaccaagc ggggcggcaa tcggagcgat aaaaccgtgc gcgtctacat
     1921 gatcgacgga tcgccatata agaagctact ggcggtgaat gggcagccgc tttccgcgca
     1981 acaagaagcg gcggaaaaag ccaaaatgca acacgagatt cggcggcggg ggcgggaatc
     2041 cgccagtgcg cgggccagcc gggttgccga ataccaaaaa gagcgcacgc gggaccacgc
     2101 aatgatgaag gaaatggggc aggcattcgt gtataagcta cgcggcgaac agaagctcaa
     2161 tggtcacgat gtgtacgtca tggatgccac accgaagccc ggctaccaac cgaaaacccg
     2221 cgagacgaag gtcctaacgg gaatgaaggg gaccttgtgg atcgacaagg aaacgtatca
     2281 atgggtgaaa gtcgaggcgg aggtagtaca gccggttaat ttcttcggat tcatcgccaa
     2341 agtcggtccg ggaacccgct tcatgctaca acaggaacca gtcggccccg atcagaactg
     2401 gatgccgacg cgcttcagca tgaaggtaaa tgcaaccgtg cttgggattt tcaacgaaga
     2461 ttcgaccgac gatgaaacat tcaccgatta tcagccgatg aataagtcca atctaacggc
     2521 cgaattaagc gagtaactgg caactcagcg gaaatcttcg agttcctcgc gggcggcgcg
     2581 aaaaacgtca cgcagcattt caaatgtcag cacgcccgtg gaagtgttct gctggctggg
     2641 atgataagaa gtgattagca ccggaagatg cggatctatc cggtgctttt tgttatggcc
     2701 gaaggtgaaa gccgaccggc gggcgatgag tccccgttcc tgaagaatcg ttaagtagga
     2761 atcgaatgcg atcctgccga gagcgacaac gcaccggacg cgcggcagca gatcgatttc
     2821 ctgggagagc cagggccggc aagcccgaaa ctcttccggt gtgggtttgt tgcccggcgg
     2881 cgcgcagcga accgaggccg taacatacgc attttgcagc ttgagcccat cgccgatgtg
     2941 tgtgctgaaa gactgcgagg cgaagccggt ctcatggaga gcgcggtaaa ggaaatcgcc
     3001 cgaacgatcc ccggtgaaca tgcgcccggt ccgattggct ccgtgggcag cgggcgccaa
     3061 gccgacgatc agcagctccg ctgcggggtc tccgaagccg gggaccggtt taccccaata
     3121 ggtccaatcc atgtaagccc gccgcttggt tcgcgcgact tcggcgcggt aggcgactag
     3181 gcgcggacag atctcacagg aa
//
LOCUS       DATNDT010000207         2242 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1252408, whole genome shotgun sequence.
ACCESSION   DATNDT010000207 DATNDT010000000
VERSION     DATNDT010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1252408"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..372)
                     /locus_tag="VN788_06730"
     CDS             complement(<1..372)
                     /locus_tag="VN788_06730"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase N-terminal
                     domain-containing protein"
                     /protein_id="HXU20258.1"
                     /translation="MIRLFGAALTAALVLLFPFSLASQPPRNAASQESGLQGCFGFVS
                     EGAGGSPTTEDASAQHGFNLANLDRSVSPCDNFYEFADGGWMKNNPIPPDRASWATFD
                     KLRDHNDSLLHGILEEASKDTK"
     gene            569..1579
                     /locus_tag="VN788_06735"
     CDS             569..1579
                     /locus_tag="VN788_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU20259.1"
                     /translation="MSERAAIRNGSRKHGLACAALIFLGCLLTPSCIRAQIEPPGPTT
                     PPPSSDDHFKIHKGRPIRTETELTLVGASVTDPLGRIVTGLEQKDFRVFENGVEQEIV
                     SFMSEDVPVSIGVIFDMSGSMSDKIDKSRQAAVQFFRTANPQDEFFLVDFNDRAQLVS
                     PFTESVDDLQNRLMYVGARGLTALYDGVYLGLSQMRSARNTKKALLIISDGGDNHSRY
                     TETEIRRFVREADTQIYAIGLFEPGGGPTPEERGGPSLLADLTAMTGGRTFVVSNLNE
                     LPDIASKISMELRNQYVLGYRPAKRVHDGKWRKIKVKLRPPKGLPPLTVSAKSGYFAP
                     EQ"
     gene            1593..>2242
                     /locus_tag="VN788_06740"
     CDS             1593..>2242
                     /locus_tag="VN788_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU20260.1"
                     /translation="MLRILLIAFLAVCPCALAQNPPPILAPSSPPPPPTTPQSPDHRL
                     KANVDLVVLHVTVTDEKGQFVSDLKESSFRVFENKAEQKISFFSRDDIPVTMGLVIDN
                     SGSMREKRAQVNAAAMTFVRTSNPQDEAFVVNFNDEYYLDTDGEFTNDQKNLNEALSR
                     IDTRGSTALYDAVIGSLGHLKKGHKDKRVLLVVTDGDDDASEKSFEDAVKAAEQSNA"
BASE COUNT          534 a          585 c          689 g          434 t
ORIGIN      
        1 tttggtgtcc ttggaagctt cctcgagaat tccatggagg agggaatcgt tgtgatcgcg
       61 gagtttgtcg aaagtggccc aggaagcgcg atcgggcggg atcggattgt ttttcatcca
      121 gccgccgtcg gcaaattcgt aaaagttatc gcagggggaa acagagcggt caagattggc
      181 aagattgaag ccgtgttggg cgctcgcatc ttcggtggta ggggagccgc cggcgccctc
      241 gctgacgaaa ccgaaacagc cctgaaggcc tgattcctga gacgcggcgt tgcggggagg
      301 ctgcgacgca agcgagaacg gaaagagcaa taccagcgcc gccgttaatg ccgcgccaaa
      361 caggcgaatc atcaggaacc tctctctgaa tagaactttc gagcgcgcaa gggaacgaac
      421 gattgttgcg cgacctacag cctgcgactg taccaccgag cattttgaga gggtggggac
      481 ggggtgtcaa ggcaccaaca atttgaggca aggttgattc ctagcgggct gaaagtgcta
      541 caatcagccc gaaggcgttg ggggcagtat gagcgagcgc gctgcgattc gaaatggaag
      601 tcgcaagcac ggcctcgcct gcgcggcgct gattttcctg ggctgcctcc tgacgccatc
      661 ttgcatccgc gcgcagatag aaccccccgg tcccacaact cctccgccat cttccgacga
      721 tcacttcaaa attcataagg gccgcccgat ccgaacggaa acggagttga ccctggtggg
      781 cgcttcggtg accgatccgc tggggcgcat cgtgacgggg cttgagcaga aagattttcg
      841 agtgttcgag aatggcgttg aacaagagat cgtcagcttc atgagcgaag acgtgccggt
      901 ttccatcggc gtgattttcg acatgagcgg aagcatgagc gacaagatcg acaagtcgag
      961 gcaagcggcg gtgcagtttt tccgcacggc aaatccgcag gacgaatttt tcctggtgga
     1021 cttcaacgac cgcgcccaac tggtcagccc attcacggag agcgtggacg acctccagaa
     1081 ccggctgatg tacgtgggcg cgcgcgggct aactgcgctc tacgatggag tctatctggg
     1141 cttgagccag atgagatcag cacgcaacac aaagaaagct ttgctcatca tatcggatgg
     1201 aggggataat cacagccgat acaccgaaac cgaaattcga aggttcgtgc gggaagcgga
     1261 cacgcaaatt tatgcgatcg ggttgtttga gcccggtggc ggtccgacgc cggaagagcg
     1321 tggcgggcct tcgcttctgg cagacctgac tgcgatgacc gggggaagaa cgttcgtggt
     1381 gagcaacttg aatgagcttc cggatatcgc ctcgaaaata agcatggagc tgcgaaatca
     1441 gtatgtcctc gggtatagac cggccaaacg agtacacgac ggcaagtggc gaaagattaa
     1501 ggtcaagctg cgcccgccca agggcttgcc gccactcacc gtttcggcga agagcggata
     1561 ttttgctccc gaacagtagc cggctcaatc ccatgctgcg cattctactg atcgcgtttt
     1621 tggccgtgtg cccgtgcgcg ctggcacaga atccaccgcc aattctggcg ccgtcaagcc
     1681 ctccgccgcc cccgacaact cctcaatcgc ccgaccaccg tctgaaggca aatgtggacc
     1741 tggtggtgct gcacgtgacc gtgaccgatg agaagggcca gttcgtttca gatctaaaag
     1801 agagcagctt tcgagtattt gaaaataaag cggagcagaa gatttccttt ttctcccgcg
     1861 acgatattcc cgtgaccatg ggtctggtga tcgacaacag cggcagcatg cgcgaaaagc
     1921 gagcgcaagt gaatgccgcg gcgatgacgt tcgtgcgcac gagcaatcct caggacgaag
     1981 cgtttgtggt gaattttaac gacgaatact atctggatac ggatggcgaa ttcacaaacg
     2041 accagaagaa cctgaatgaa gcgctttcgc ggattgatac tcgcggtagc acggcgctat
     2101 acgacgcggt aatcggatcg ctaggtcacc tgaagaaggg gcacaaggat aagagagtgc
     2161 tgctggtagt taccgatggc gacgatgatg caagtgaaaa gtcgtttgaa gacgcggtga
     2221 aggccgcgga gcaatccaac gc
//
LOCUS       DATNDT010000208         6876 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_943581, whole genome shotgun sequence.
ACCESSION   DATNDT010000208 DATNDT010000000
VERSION     DATNDT010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943581"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="VN788_06745"
     CDS             complement(<1..701)
                     /locus_tag="VN788_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20261.1"
                     /translation="MRKKLPVAVGCLAIVTMGLAVARPRRVRAANNAQDGVAPGCDRT
                     CLYGFLDQYLDALKMKDPSRLPLAKNFRYSENNVIMDIGDGVWGTVTGLGNYNLRMAD
                     PTNGEVGFYGIVDETNATSTFALRLKVEDGKISEAENLIRRSEGGGPFPSKPNLVEKP
                     VLNEIVPPEARRPRARMIALVDGYFSTLQLNDGVLFTQFDDNCNRIENGLQTTHNPDL
                     MKIIPVANLGCAQQF"
     gene            934..1824
                     /locus_tag="VN788_06750"
     CDS             934..1824
                     /locus_tag="VN788_06750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU20262.1"
                     /translation="MGNEKRFDVDRLKAIDVHAHIEHEGQGTETDQAAKEYFGGAAAR
                     GRKEMAEYYRSRNIGCVVFTVDERLTGRPVVPNDDVIEFAAQNSDIMIPFASVDPTRG
                     PEAISEAKRLIATGMLRGFKLHPPLQQFFPNDRVAYPLYKVIEEAGLPVVFHTGHSGI
                     GTGMPGGGGIRLKYGNPMYIDDVAVDFPDMPIIMAHPSFPWQDEAISVCLHKRQVYID
                     LSGWSPKYFAPNLIQYANSLLKTKMLFGSDYPLITPDRWMADFEKAPFKDEVRPLILK
                     ENAMKLFGLTSKTAAPSARD"
     gene            1932..3884
                     /gene="acs"
                     /locus_tag="VN788_06755"
     CDS             1932..3884
                     /gene="acs"
                     /locus_tag="VN788_06755"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HXU20263.1"
                     /translation="MQEFDPHVLDEKAVPPPEDFRARARVRSLEQYREMYEQAEREPE
                     LFWRERAKMIDWFEPPAKVLEWDLPHAKWFTGGKLNVAYNCLDRHLKKNLNKPALLWE
                     AEDGSSLQFTYAELHDRVCRFANALNSLGVKPGDCVAIYLPMIPELPIAMLACARIGA
                     VHSVIFGGFSATALADRIADAKSKILITADGGFRRGKIVPLKETADAALRNCPTIEKS
                     VVVRRTEEKVAWHEGRDLWWHDLEKAASAECAAESFDSEHPLYILYTSGTTGKPKGVL
                     HTSAGYLLQTMWTAQIVFDLRDDDVYWCTADIGWVTGHSYIVYGPLANGATVLMYEGA
                     PDHPRRDRFWEIVQRYKVTIFYSSATAIRAFMAWGTDWIDSHDLSSLRLLGTVGESIN
                     PAAWKWYFEAIGKNRCPIIDTWWQTETGAMMISPLPGATPAKPGSATLPLPGVIAKVV
                     NMQGQPVAPGETGYLTIQKPWPSMLRTIYGDDERYVRDYWKKIPGVYFAGDAAQCDKD
                     GYIWVLGRVDDVIKVAGHRLSSMEIESALVSHTAVAEAAVVARPDEIKGEAIVCFVTL
                     RSGQSASGGLRTALMDHVSTTIGTIARPADVRFCDILPKTRSGKIMRRLLREVAHKGG
                     ISGDTSTLEDMTVIERIAKAQGLEEE"
     gene            4073..6019
                     /locus_tag="VN788_06760"
     CDS             4073..6019
                     /locus_tag="VN788_06760"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HXU20264.1"
                     /translation="MFTFLGRTIVFAAALILACILPQPTKAQRAGDWPMYSHDLASTR
                     YSPLKQINTGNVATLTQAWSYPMRPGGKGPSAITFSEVTPIVVNDVMYLAAGNRILAL
                     EPETGKEIWSFEVDKGLVPQRGVAYWPGDSNNPPRIIFTSLHRLMALNATTGKLDPKF
                     GNNGEVEMDVPFAEVPTIYKDIIIVGANVYGPGETNLHPQDEVGAGVPGDSRAYDART
                     GKKLWTFHTIPQPGEIGHDTWGDDSWQGRGGTNVWSITMTVDQKRGIVYMPIGGPAAN
                     YYGGDRPGNNLFSNSLVAVDVKTGKLKWYFQTVHHELWDFDLPPEPVLIDIVHNGQKV
                     PAVIQMGKSPYMFILNRYDGKPVFGVQERPVPKGDVPTEQYSPTQPFPVKPPPLARDS
                     FSAKDLVTADDTTPEHAQACKQLLDSYGGLYNAGPFTPWAFRPEGAQPKASLVFPGAT
                     GGTNWGGTASDPTTGHVFLNVQNSGSTGWIEKNPKYQPESGGPELPYHRGSGAGSQYG
                     AFSAIAKDASGHSLGNLPCQKPPWESLLAVNANTGDIVWQVPLGITEGLPSGKQNTGR
                     PGAFAGPIVTGGGLVFIGATNDDRFRAFDSKTGKQLWSIKLDYAATAVPMTYEGKNGK
                     QYVAIVDAGGGSGDKQALLVFALP"
     gene            6153..6249
                     /locus_tag="VN788_06765"
     tRNA            6153..6249
                     /locus_tag="VN788_06765"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6188..6190,aa:Sec,seq:tca)
     gene            6303..>6876
                     /gene="selD"
                     /locus_tag="VN788_06770"
     CDS             6303..>6876
                     /gene="selD"
                     /locus_tag="VN788_06770"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HXU20265.1"
                     /translation="MPADETVLKLTALSSCAGCASKLSRQALTQVLHQVPRLKDRNVL
                     VGAATGDDAGVYRIDRQRALVQTTDFFTPIVDDPYSYGQIAATNALSDVYAMGGRPLT
                     ALNLAGMPADTISLDVVNQILRGGAEKIKEAKCVLIGGHTIKAPEPIFGFAVTGVVSP
                     QRMMTNANARPGDTLVLTKPLGTGVITTAIK"
BASE COUNT         1537 a         1940 c         1998 g         1401 t
ORIGIN      
        1 ttgaattgct gcgcgcaacc caggtttgcg acggggatga ttttcatgag gtcgggattg
       61 tgagttgttt gcaaaccgtt ctcgatgcga ttgcaattgt cgtcgaactg ggtgaagaga
      121 actccgtcat tgagttgaag cgtgctgaaa tagccgtcga caagcgcgat catgcgggcg
      181 cgcgggcgtc gcgcctccgg cggaacgatt tcgttcagca ccggtttctc gaccaggttc
      241 ggtttgctcg gaaacgggcc gccgccttcg ctgcgccgga tcaggttttc ggcctcggaa
      301 atttttccgt cttccacttt gagccggagc gcgaaggtgg aagtagcatt ggtctcgtcc
      361 acgattccat agaagccaac ttcgccattg gtaggatcgg ccattcggag attgtagttg
      421 cccaggccgg ttaccgtgcc ccacacgccg tcgccaatat ccattatcac gttattttcg
      481 gaatagcgaa aattcttggc cagcggcaag cgcgagggat ctttcatctt cagcgcgtcg
      541 agatattgat cgaggaagcc gtacaggcaa gtccggtcgc aaccgggcgc aacgccatcc
      601 tgcgcgttgt tcgccgcgcg aacgcgcctc gggcgcgcca ccgccaggcc cattgtcaca
      661 attgccagac agccgacggc cacaggcagc tttttacgca tgatttcgtc ctccggattg
      721 aaccggcggg aagatactac atcgagaaaa aactacaagg ggtttccata cgcaggatgg
      781 tcccgccgac tcattaatgc tcgcctgttg aggaatgact gggaaaaaca gataatctgg
      841 tggcaaaatc gggcggccct tttcggcgtt gggccatctg aacgaactga accatctcat
      901 gatcgaactg caccaatcca aacagggggg gccatgggga atgaaaagcg tttcgacgtg
      961 gaccggttga aagcgattga cgtccatgcg cacatcgagc acgaaggaca aggaactgaa
     1021 accgatcagg cggctaagga gtatttcggc ggcgcagccg cgcgaggccg caaggagatg
     1081 gcggagtact atcgctcccg gaacatcgga tgcgtggttt ttaccgttga tgagcggctt
     1141 actggcagac ccgtggtgcc gaacgatgac gtgattgagt ttgccgcgca gaattccgat
     1201 attatgattc ctttcgcgag cgtggatccg actcgcgggc cggaggcgat cagcgaagcg
     1261 aaacggctaa tcgcgactgg aatgctccgc ggattcaagc ttcatcctcc cttgcagcaa
     1321 tttttcccga acgatcgggt agcctacccg ctgtacaagg tgattgagga ggcgggactg
     1381 ccggtggtgt ttcacacggg acacagcggc ataggcaccg gaatgcccgg cggcggcgga
     1441 atccgattga aatacggcaa tccgatgtac attgatgatg tggcggtcga ttttccggac
     1501 atgccgatta tcatggcgca cccgtcgttc ccctggcagg acgaagcaat ctcggtttgc
     1561 cttcacaagc ggcaggtgta catcgatttg tcaggctggt cgccaaaata cttcgcgcca
     1621 aatttgattc aatacgcgaa ttcattgttg aagacgaaaa tgctattcgg ctcggactat
     1681 ccgctgatta ctcccgaccg gtggatggct gatttcgaga aggccccctt caaggacgag
     1741 gtgcgaccgc tgattttgaa agaaaatgcc atgaagttgt ttggcctcac gtccaaaacc
     1801 gcagcgccca gcgcaagaga ctaggcgtag agagccatca gggaagcata tcgtccctgc
     1861 cgcttaaata ttaggtagat tgcgatacca acggcgcccg ctccgatacg ccccgccata
     1921 aaggaggagt catgcaggaa ttcgatcctc acgttctcga cgagaaggcc gtgccgccgc
     1981 ccgaagattt cagggcgcgc gcgcgagtgc ggtcgctcga gcaatatcgc gaaatgtacg
     2041 agcaggcgga gcgggagccg gaactgttct ggcgcgagcg agcgaagatg attgactggt
     2101 ttgagccccc cgcgaaagtg ctcgaatggg acttgccgca cgctaagtgg ttcacgggcg
     2161 gaaaactgaa cgttgcctac aactgcctgg atcggcactt gaaaaaaaat ctcaacaagc
     2221 cggctttgct ttgggaagcg gaagacgggt cgtcgcttca attcacttat gccgagttgc
     2281 acgaccgcgt gtgccggttc gcgaacgcac tgaattcgct gggcgtaaaa cccggcgatt
     2341 gcgtcgcgat ttacttgcca atgattccgg agctgccgat tgcgatgctg gcgtgcgcgc
     2401 gaataggggc cgtacattca gtgatctttg gcggatttag cgccaccgcg ctagccgatc
     2461 gaatcgcgga cgcaaaatct aaaatactca tcaccgcgga cggaggtttt cgccgaggga
     2521 aaatcgttcc actgaaagaa acggccgatg ccgcgctcag aaattgcccc accatcgaaa
     2581 aatcagtcgt cgtgagacgc accgaagaaa aagtcgcctg gcatgaaggc cgtgacctct
     2641 ggtggcacga cctcgaaaag gccgcttcag ccgagtgcgc cgcggagtcg ttcgattccg
     2701 agcatccgct gtacattttg tacaccagcg gcaccaccgg aaaacctaag ggcgtgctgc
     2761 acacttcagc cggctacctg ctgcaaacaa tgtggaccgc acaaatagtt ttcgatctgc
     2821 gggacgacga cgtgtactgg tgcaccgccg atatcggatg ggtgacgggg cacagttaca
     2881 tcgtttacgg gccgctcgcg aatggcgcca ccgttctcat gtacgaaggt gcgccggatc
     2941 atccgcgccg cgatcgattt tgggaaattg tgcagcgcta caaagtcacg attttttaca
     3001 gctccgctac cgcgatccgc gcgttcatgg cctggggcac ggattggatc gactcgcacg
     3061 atctgagcag cctaaggctc cttggcacag taggagaatc gatcaatccg gcggcttgga
     3121 aatggtattt cgaggcgatt ggaaaaaatc gatgcccgat catcgatacc tggtggcaaa
     3181 cggaaaccgg cgcgatgatg atttcgccgc tgccgggagc gactcccgca aaacccggct
     3241 cggccacact gccgttgccc ggagtgatcg cgaaagtggt gaacatgcag ggacagcccg
     3301 tagcgccggg cgagaccggc tacctcacca ttcagaagcc gtggccttcg atgctgcgca
     3361 caatttatgg agatgacgag cgctacgttc gcgattactg gaaaaagatt cccggagttt
     3421 atttcgccgg cgacgccgcg cagtgcgaca aagacggata catttgggtt ctcggtcgtg
     3481 tggacgacgt aatcaaagtt gccggccacc gcctcagctc gatggaaatt gaaagcgcgc
     3541 tggtcagcca cacagccgtg gccgaagccg cagtggttgc gcgcccggac gagatcaagg
     3601 gggaagcgat cgtctgcttc gtcacattgc gctccgggca atccgcttcc ggcggtctgc
     3661 gaaccgcact gatggatcac gtctccacaa ccattggaac gattgcgcgt ccggcggacg
     3721 ttcgcttctg cgatattctg ccgaaaacgc gcagcggaaa aatcatgcgg cgcctgctgc
     3781 gcgaagtggc gcacaaaggc ggcatctcgg gcgacacgtc cactctcgaa gacatgaccg
     3841 tgattgagcg gattgcaaaa gctcagggcc tggaagagga atgacgctcc aacattcaat
     3901 ccgaaacaag tagccgcaaa caatttgcac taatttcgct ctagctcact aattgcagcg
     3961 attctgccat cgcacccgac gcgctttctt gtatatactt cacggctcga cacagcgcag
     4021 cgccgcacgc tgcgtcgtcg ttttggaagc cgaattttta gtagggggcc tcttgttcac
     4081 atttttagga cgcacaattg tcttcgcggc tgcgttgatt ctcgcctgca ttcttcccca
     4141 gcccacgaaa gcgcaaaggg ccggcgactg gccgatgtac agccacgatc tggccagtac
     4201 tcgctattca ccgctcaagc agattaatac tggaaatgtt gcaacgctca ctcaggcctg
     4261 gtcgtatccg atgcggccgg gaggaaaggg tccatccgcc atcaccttca gtgaagtaac
     4321 tcccattgtc gtgaacgacg tgatgtatct tgccgccggc aatcgcatac tcgcgctcga
     4381 acccgaaacc ggcaaggaaa tttggagctt cgaagttgat aaaggcctcg ttccgcagcg
     4441 tggcgtcgcc tattggccgg gagactcgaa caatcctccg cggattatct tcaccagctt
     4501 gcacaggctg atggcgctca acgccactac cggcaaactc gatccaaagt ttggcaataa
     4561 tggcgaagtc gaaatggacg tgccgtttgc cgaggtcccc acaatttaca aggacatcat
     4621 catcgtgggc gcgaatgtgt acgggccagg cgagacaaat ctccatccgc aggatgaggt
     4681 tggtgcggga gtccccggcg attcgcgcgc ctacgatgcg cggaccggca aaaagctgtg
     4741 gacgttccac actattccgc agccgggtga gatcggccac gatacctggg gcgacgatag
     4801 ctggcagggc cgcggcggca ccaacgtctg gtccatcacc atgacggtgg accaaaaacg
     4861 cggcatcgtt tacatgccga ttggcggtcc ggcggcgaac tattacggcg gagaccggcc
     4921 cggaaataat ctcttttcca attcactggt cgcagtcgat gtaaaaaccg gaaaattgaa
     4981 atggtatttc caaaccgttc atcacgaact gtgggacttc gatttgccgc ccgagcctgt
     5041 gctgatcgat atcgttcata acggacaaaa agttccggcg gtcatccaga tgggtaaatc
     5101 tccatacatg ttcattctga atcgctacga tggcaagccg gtatttggcg tccaggagcg
     5161 tccagtgccc aagggcgacg tgccaaccga gcagtattcg cccacgcagc catttccggt
     5221 gaagcctccg ccgctcgcgc gagacagctt ttcggcgaag gatttggtca ccgcggacga
     5281 tacaactccc gagcacgcgc aggcctgcaa gcagctgttg gatagctatg gcggtcttta
     5341 caacgccggt ccttttacgc cgtgggcatt tcggcccgag ggtgctcaac caaaggcctc
     5401 gttggttttt cccggggcga caggaggaac gaactggggc gggaccgctt ccgatccaac
     5461 aacgggtcac gtgttcctga atgtgcagaa ctcaggttca acgggctgga tcgaaaagaa
     5521 tccgaaatac cagccggaat cgggtggccc cgagcttccg tatcatcgag gcagcggagc
     5581 cggcagccag tacggcgcgt tcagcgcaat tgccaaggac gcaagcgggc acagcctcgg
     5641 caacctgccg tgccagaaac cgccctggga aagcctgctt gctgtgaatg cgaacaccgg
     5701 agatattgtc tggcaggtgc cgctgggaat taccgaaggg ctgccttccg ggaagcaaaa
     5761 taccggaagg ccgggagcct ttgccggtcc aattgttaca ggcggcggcc tggttttcat
     5821 cggagcaaca aacgatgatc gctttcgcgc tttcgattcc aagacgggca agcagctctg
     5881 gtccatcaag ctcgattacg ctgcaaccgc cgtccccatg acctatgaag gaaaaaacgg
     5941 caagcagtac gtggcgattg tggatgcagg aggtggttcc ggagacaagc aggcgctact
     6001 ggtttttgcc ctgccgtaat gtggcgcagg cactcttgcc tgcgcgtgat accgcgcgcc
     6061 cgttctttaa aaaatcttct gcgaatagtc gaatggagta tcctatgcgg cgcaatagcc
     6121 agtggaattt gctgattcgc cgctgtttcg ccggagagtg atgcgtgcct ggtggtcgtc
     6181 gcggtcttca aaaccgctgc cccgacactt cgtgccgggg ggtaggttcg attcctaccc
     6241 tctccgccaa attttccaaa gtcgtatact agttgtgggt ttcaccgagg atctaaccgc
     6301 tgatgccggc tgacgaaact gtactaaagc tgactgcact ttcatcctgc gccggttgcg
     6361 catccaagct cagccgccag gcgctgacgc aagtattgca tcaggtgccc aggctgaagg
     6421 atcgaaacgt cctggtcggg gcggctaccg gcgatgacgc cggcgtttat cgcatcgacc
     6481 gccagcgcgc gctggtgcaa accacggatt ttttcacccc catcgtggac gatccctatt
     6541 cctacggaca gatcgcggca accaacgctt tatccgatgt gtacgcgatg ggcggacgcc
     6601 cgctcaccgc gctaaatctg gccgggatgc ctgccgatac aatttcactc gacgtggtaa
     6661 atcaaatcct gcgcggcggc gccgaaaaaa tcaaggaagc aaagtgcgtc ctgatcggag
     6721 ggcacaccat caaagcgccc gagccaattt tcggattcgc tgtgaccggc gtggtgtccc
     6781 cccagcgcat gatgaccaac gccaatgccc ggccgggcga taccctcgtg ctgacaaagc
     6841 cgctcggcac cggcgtcatc accaccgcca tcaagc
//
LOCUS       DATNDT010000209        10900 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1182534, whole genome shotgun sequence.
ACCESSION   DATNDT010000209 DATNDT010000000
VERSION     DATNDT010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1182534"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="VN788_06775"
     CDS             complement(<1..396)
                     /locus_tag="VN788_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20266.1"
                     /translation="MYNREAARYARWAAWTAALIALIVAGVYGGRVVREARARRHAPA
                     PVPASVQQQSAQISFSKVEQNRTVFTIRASHATQYKEGNRSLLEDVWITIYGPGGKRD
                     DNIHTRKCSYEPETGDVRCEGVVQIDIGKA"
     gene            complement(464..1627)
                     /locus_tag="VN788_06780"
     CDS             complement(464..1627)
                     /locus_tag="VN788_06780"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXU20267.1"
                     /translation="MPMLPSEFQDATKHAPPERQRRVSDGKPFDGVERRHNPTVPLEP
                     PDISEVAVFRELGKALTSSLQLDQVLRTIMEKINEVLHPDTWSLLLMDLAKNELYFQI
                     ATGQGSEALKDMRIKVGQGVAGWVAQSGEPVVVADTSVDSRFFGQADEKTKVSTRSIV
                     AVPVRFREQCLGVIELINCIGPEGFSPRDLALLQALADYAAIAIENARHVQRIHELTI
                     TDDCTSLYNARHLNFMLDTEIYRSHRYSFEFSVIFIDLDHFKSVNDTHGHLMGSKLLA
                     EIGEAIKEKCRMIDLAFRYGGDEFVVLLPQTSKENALGVARRLHNLIRETRWLKDTGL
                     DVTVTASVGVASYPTDSRTKAELLHLADEAMYLVKNTTRDCVAAAGVGVLPAP"
     gene            1766..2560
                     /locus_tag="VN788_06785"
     CDS             1766..2560
                     /locus_tag="VN788_06785"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HXU20268.1"
                     /translation="MSSTPFGEHLKREREMRGVSLNEISAATRISTRFLEAIENDRWN
                     ELPGGAFNRGFIRSIARYLGLDEDSIVAEYSLDTSGSANVREAAQFVKTGRNWRPVVV
                     AAVMLLALLGAGLLVHHFFGSRIVAMLHGRQSLTTSAARSRPASPDIQPSVPNQTKAQ
                     PGPGSADSQESDPAPEGTLALSIRTEKPGYLKVVTDGQTVLHERVRAGYSHNFQARNA
                     FEIFSSRASGVRLSLNGQNVPFPPTPGRMARIELSKNDLRSSGGVH"
     gene            2544..3509
                     /locus_tag="VN788_06790"
     CDS             2544..3509
                     /locus_tag="VN788_06790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20269.1"
                     /translation="MEESISSDLREQIRSGRARPEQKMAICAGGTPLAPEDRVELLAV
                     LAADSDPEIAERAQSALLSRPTEHFLAACARPDADPRLFAYCSDNLPDKKGIADTLAR
                     NASCPTANVARIASHLTSAGIQALLDNLERFISDPQLVRAVSASPASTAEQRELLGEM
                     QKGALTTHEIEEAAAEIEPDPIKRQTLFQKVAHMNVVQRLTLALKGGRSERMLLIKDP
                     NKLVQRCVLQSPRLTETEVEAFSAMTNLSGEILRTISMTRVFIKSYTIMKNLVNNPKT
                     PLDASLHMFPRLTATDLMKLTANKNIPDTLRSAALKLHRKRKMGG"
     gene            3577..4767
                     /locus_tag="VN788_06795"
     CDS             3577..4767
                     /locus_tag="VN788_06795"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HXU20270.1"
                     /translation="MISPAAAPTVREGSVVISSRGVERLRSGHLWVYRSDISSVQADP
                     GAVVRLSDDRGRFQGRAFYSDKSQISIRLLTRSDIPIDRAFFAERIERAAAYRKLVVE
                     DSDACRLVYSEGDLLPSLIVDRYGEYLVIQTLSQSTERLKGEFVDILVEMFAPKGVLE
                     RNDPKVRLLEGLDQRVGALYGEVPTEVLAKENGITFVHDLAKGQKTGSFLDQRENHRA
                     ARRYASGDVLDCFSYEGGFALTVADKCSHVEGIEMAPAALEAARRNQKLNAISNAHFR
                     EGNTFDLLKEYDEAGRRFQMVILDPPAFAKNRDSIPAARRGYKEINLRALKLLTPGGF
                     LITCSCSYHISEALFLQILAEAANDAGRTLSIVERRTQAQDHPIVLTMPETHYLKCLI
                     IRAF"
     gene            5041..5655
                     /locus_tag="VN788_06800"
     CDS             5041..5655
                     /locus_tag="VN788_06800"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXU20271.1"
                     /translation="MSSKNRKSQSGFSMIELMVVLIVILVVAGYAVPNILSFIHMTKL
                     RGSASDYASILQVGRIRSVQDNKFYSIYLVAGSPQEAYVDLTNNGGTVAAPGDPLIAL
                     SGEVVPVAAGGAPDATDLQNKFLPAGAPAPNDGSAVPVIFSSHGLPCTTQAATGGNVC
                     TGALATAFWVFFQDTVTSSWEAVTVSPAGRIQKWQHGSSDWTKL"
     gene            5704..6267
                     /locus_tag="VN788_06805"
     CDS             5704..6267
                     /locus_tag="VN788_06805"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HXU20272.1"
                     /translation="MRHKAERGMTLIELMIAMVVLAIGLGGITTLLVTAIASNNKNST
                     DTAATLLAQMVVDQISSQNIYAPNNVLVSDCVGNQLAITTAPGAVGTGTGATLKADGT
                     IDFTQAYSAIPAGYAMRYTVCSSSVGGIPMTYEVRWNVMKVSTNTTTRMITAAARPLA
                     SNANQLGGFYFALPVNLRSIGAPSAGQ"
     gene            6291..6959
                     /locus_tag="VN788_06810"
     CDS             6291..6959
                     /locus_tag="VN788_06810"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXU20273.1"
                     /translation="MNAERQGGFSLIELLVSLAILSIVIGVVLDGINTMQARNTVETN
                     KLDLTQQTREFMDQIINDLDQAGFPSLSMFDPVGLTSASDCTQDNNVACGFKYLSTSE
                     IQFEGDVDGSGVSYVDIKLNPPGGPCPCTIQRGAIAKPGTTPLFYTQVDNVMNTAVFT
                     AYDSGGNNLDLTAPITGAGGFDNITAIGITLYVKSSQPDPKTGQYPTVTMVSTAKVNN
                     IQSY"
     gene            6994..8805
                     /locus_tag="VN788_06815"
     CDS             6994..8805
                     /locus_tag="VN788_06815"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HXU20274.1"
                     /translation="MNNAARTKERGIALFFAIFALLLLTAIGAALIFMSSTETSVNSN
                     YRQEQVAYMGAKAGIEEARARMMASDPASIVCGATVGPCSQPVIGNASYPLFDSSAIK
                     QATPPSAANHMIYYILNPGGGSAVQPWSSGTRYADDELCHDGYSGLFSVSQVVAPDVR
                     CSATPSGTGWYDSYTSTLPFNGTSKALPYKWVRIAPKLNGSISYLTGTGSNVATVPYY
                     VNQTFSGSVDAFTPICWDGHEEKALTTQTVPIVINYCSQMTSNAVGFAGAYMTNVYTV
                     TALGVSSDGPNAARKVVQADVALQPTTPFPYGLYATSTACPAITFTGQHPSTDSYTTA
                     GGGTYATTRSSTGGDIGSNGGVSVQNGNIGGIVGVLQPPPTGNGTCATPLSINSPGGT
                     MVGTTTCPTGDNSNPPASCYLPTPYTFQTPPRPSPLPPVTNYTPPNCAGGKCMTPGLY
                     GNINVNGTLYLAPGIYNVNSLSMQGNGAIIVNPPGSVTLNVAGCANSTCSSTLGNPLS
                     IAGNGITDDAIANDFTINYAGTGTVSIAGNGNVTAILNAPNAPVTQQGNGAWYGSILS
                     STASIGGNAFFHYDKNAALAPNNNGYYTLIGYREVPY"
     gene            9591..>10900
                     /locus_tag="VN788_06820"
     CDS             9591..>10900
                     /locus_tag="VN788_06820"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXU20275.1"
                     /translation="MPNGQRLVVVEPNGTRRDVVVSASPFRIGRQAGNELTLRDSRVS
                     RHQAQILTVEGKLVLEDMGSSHGTFVNGEKILRHELKPSDRIDFGVADSYHVIYVGDG
                     ATIEEIVGRIEAPAPEQSGPRELHHLGVLLEVARALSSGLALEDILTAVLDAAIVLTR
                     TERGVLLLYSSSGEVQTAVARNAQGVSLRTEDVQISQGVLKRVASTRRELIVSETEEG
                     SGLTSQQESMARLALRTIVAIPVDKLPVIEALDATISARQGELLGILYLDSRAPSSAF
                     SALDREVLRTLAREAATVVENARLFAASRAKARLDHEMEIASQIQQNLLPKTLPNLPN
                     VAVAGSTLACYSVGGDCFDVIALDGGRHGFFVGDVSGKGITAALLATLLQGVFFTTAA
                     MDIPLPGIFSRVNQYLCERSGDDRYATVFYGVLDRTGRFEYVNGG"
BASE COUNT         2388 a         3260 c         2975 g         2277 t
ORIGIN      
        1 tgcctttcca atgtcgatct gcaccacgcc ctcacagcgc acgtccccgg tttccggctc
       61 gtagctgcat ttgcgggtgt ggatgttgtc gtcgcgcttg ccgccggggc cataaatcgt
      121 gatccacaca tcttctaata gcgaacggtt tccctcctta tactgggtgg catgagaggc
      181 gcgaatggtg aagacagtgc ggttctgctc tacttttgag aaggagattt gagccgactg
      241 ctgttggacg gaggccggta caggagcggg agcgtgcctg cgagcccgcg cctcgcgaac
      301 cactcgaccg ccgtacacgc cggccacgat caaggcgatc agcgccgcgg tccacgccgc
      361 ccaacgtgcg tagcgcgcag cttcacgatt gtacaatctg cagttccctc ttttgagaag
      421 tataggcgcg tgttcggccg gggacaacga ggcacgggct acattaaggc gcgggcagca
      481 ctcccacgcc cgcagccgct acgcaatcgc gggtggtgtt cttgactagg tacattgctt
      541 catcggcaag gtgcagcagt tcggcctttg ttcgcgaatc ggtagggtaa gaggctacac
      601 ccacgcttgc ggttaccgtg acgtctagac cggtgtcttt tagccagcgc gtttcgcgga
      661 taagattgtg cagccgccgc gccacgccga gggcattctc cttcgaggtt tggggaagta
      721 gcaccacgaa ctcatcgccc ccatatcgga aggcaaggtc gatcatgcga cacttctctt
      781 taatcgcttc accaatctcg gcaaggagtt tcgatcccat caggtggccg tgcgtgtcgt
      841 ttacactctt gaagtggtcg agatcgataa aaattactga aaattcgaat gaataccgat
      901 gcgaccggta aatctcggta tcaagcatga aattcaaatg acgtgcgttg taaagactgg
      961 tgcagtcgtc cgtgatcgtc agctcatgaa tgcgctggac gtggcgcgcg ttttcaattg
     1021 caatggcggc gtaatcagca agagcttgta gcaaggccag gtcacgcggg ctgaatccct
     1081 cggggccgat gcagttgatt agctcgatca ctccgaggca ctgttcgcgg aagcgcacgg
     1141 ggacggcaac gatcgaccgg gtggatacct tcgttttctc gtccgcttgc ccgaagaaac
     1201 gcgaatcaac gctggtgtct gcgaccacca caggctcgcc ggactgggcc acccagcccg
     1261 ccaccccttg gcccaccttg atgcgcatat ccttcaaagc ctccgagcct tggccggtgg
     1321 cgatttggaa gtaaagctcg ttcttcgcga gatccatcaa tagcaatgac caagtgtcag
     1381 ggtgcaacac ctcgttgatt ttttccatga tggtgcgcag cacctggtca agctgaagcg
     1441 aagaggtcag cgctttgccg agttcacgaa acacagcaac ttcactgata tccggcggtt
     1501 ccagcggcac cgtgggattg tggcgccgct caacgccgtc gaaaggcttt ccatcggaaa
     1561 cccggcgctg ccgttcaggt ggtgcgtgct ttgtcgcgtc ttggaattcg gaaggcaaca
     1621 ttggcattat agtgaaccat tttcatctgt cgcaacggac aatacaacgc gattgccgaa
     1681 aggccaatgg gctcctctca gatgcgctcg ggcgtcattg acactgattc caccggcgtc
     1741 tatatttttc cgaggtcccc acggaatgtc ctcaacacct ttcggcgaac acctcaagcg
     1801 ggaacgcgaa atgcggggag tttcgctgaa cgaaatttct gctgccacac gcatcagcac
     1861 tcggttcttg gaagcaattg agaatgaccg gtggaacgag ctgcccgggg gagcgttcaa
     1921 tcgcggcttc atccgctcga tcgcacggta cctggggctc gatgaggata gcatcgtagc
     1981 cgaatactca ctcgacacgt ccggcagtgc aaacgtccgc gaggcggcgc agttcgtaaa
     2041 gaccggccgg aactggcgac cagtggtggt ggcggcagtg atgcttctcg cgctactggg
     2101 tgccggactt ctggtgcacc atttcttcgg atcacgaatc gtggccatgc tgcacggacg
     2161 ccaatccctc acgacctcag ccgcacgctc tagaccggca tcgccggata tccagccaag
     2221 cgtcccgaat cagacaaagg cacagccggg gcccggaagc gcggactcac aagagtccga
     2281 tcccgctcct gaaggaactc ttgcgctcag catccgaact gaaaagccgg gctacctaaa
     2341 ggtcgtcacc gacggacaaa cggtgttaca tgaacgggtc cgagctggtt actcccataa
     2401 cttccaggcg cgcaacgctt ttgaaatttt ctccagcagg gcaagtggtg tccggctaag
     2461 tcttaatgga cagaacgtcc cgtttccccc gactccaggt agaatggcgc gcatcgagtt
     2521 aagcaagaac gatctgcgct ccagtggagg agtccattag cagcgacttg cgcgagcaga
     2581 tccgttccgg gcgcgcccgg cccgaacaga aaatggcaat ctgcgccggg ggcaccccgc
     2641 ttgctcccga agaccgcgtt gaattactcg ccgtactagc tgcggattcg gatcccgaaa
     2701 ttgccgagcg cgcgcaaagc gccctgctga gccgtccaac cgaacatttc ttggccgcat
     2761 gcgcccggcc agatgcggat cctcgcctct tcgcctattg ctccgataat cttcccgata
     2821 aaaaggggat cgctgatacc ttggcacgca acgccagttg tcccacggca aatgttgccc
     2881 ggatagcgtc ccatctaacc tcggccggca tacaggcgct gctcgacaac ctcgaacggt
     2941 ttatttctga cccgcagttg gtgcgtgccg tgtccgcctc gccagcatct actgccgaac
     3001 agcgcgaact cctcggagaa atgcaaaagg gcgctctaac gacccacgaa attgaagagg
     3061 ccgccgccga aattgagcct gaccccatca agcggcagac tctctttcaa aaggtcgcgc
     3121 acatgaacgt ggtgcagcgc ttgacgctgg ccctgaaagg cggtcgctcc gaacgcatgc
     3181 tcttgatcaa ggacccgaac aaactggtac aacgctgcgt gctccagtct ccgcgtttaa
     3241 ccgagacgga ggttgaggca ttttcggcga tgaccaactt gagcggagag atcctgcgaa
     3301 cgatttcgat gactcgggta tttataaaga gctacacgat tatgaagaat ctcgtcaaca
     3361 atccgaaaac tccattggac gcttctctgc atatgtttcc tcgcctcacg gcgaccgacc
     3421 tgatgaagct tacggccaac aaaaatatcc ccgacactct gcggtcggcc gccttgaagc
     3481 ttcatcggaa gcgcaaaatg ggaggctgat ggaacggaac cttttctcga atcactcggc
     3541 ggtaagcatg agccccgaag gtggtcggcg cggcgcatga tttctccagc cgcagcccca
     3601 accgtacggg aaggatcggt ggtcattagt tcgcgcgggg tcgaacgcct gcgttccgga
     3661 catctatggg tttatcgcag cgacatttcc tccgtgcagg cagaccccgg agccgtcgtc
     3721 cgtttgtccg acgatcgcgg ccgctttcaa gggcgcgctt tttacagcga taagtcgcag
     3781 atctcgattc gcctgctcac acgcagcgac attcctatcg accgcgcctt ttttgcggag
     3841 cgaatcgagc gtgcagctgc ctatcgcaaa ctagtcgtgg aagattcgga cgcctgccgc
     3901 ttggtttata gcgaaggcga tctgcttcct tcgctcattg tggaccgata tggcgagtac
     3961 ctggtcatcc aaactctcag ccaatctacc gagcggctga agggtgaatt cgtcgatatc
     4021 cttgtcgaga tgtttgcgcc gaagggtgtg ctcgagcgca acgaccccaa agttcgtttg
     4081 ctcgagggcc tcgatcagcg tgtcggcgct ctctacggcg aagttccaac cgaggtcttg
     4141 gccaaagaga atgggatcac tttcgttcac gatctggcga agggtcagaa aaccggttct
     4201 tttctggacc agcgggaaaa tcatcgggct gcgcgccgct acgcttccgg ggatgtcctc
     4261 gactgtttca gctacgaggg cggattcgct ctcacggttg cggacaaatg ctcccacgtt
     4321 gaaggcattg agatggcgcc ggctgcgctt gaggctgcgc gccgaaatca aaaattgaac
     4381 gcgatctcca atgcccactt ccgcgaagga aacacgttcg atctcctaaa ggaatacgac
     4441 gaggcaggcc gccgcttcca aatggtcatc ctcgatccgc cggccttcgc caaaaatcgc
     4501 gatagcattc cagcagcccg ccgcggatac aaggaaatca atctgcgcgc cttgaagctg
     4561 cttaccccag gcggattctt gatcacctgc tcgtgctcct accacattag cgaagctctt
     4621 tttctgcaga ttctagcgga ggccgcgaac gatgccggcc gaacgctttc tattgtcgag
     4681 cgtcgcactc aagcgcagga tcacccgatc gttctcacca tgcccgagac gcattacctc
     4741 aagtgcctca tcatccgcgc cttttaggcc gatcctccgt cgcaagaagt gaactaagga
     4801 tgcgcacagt ccaggtcact gaccggtggc ggcgcggaat gctcgatcca tggccggtct
     4861 tgaaatgtta cgaaatgtta catcgccccc ctgtccgcgg ctgcgcggac gtagaacata
     4921 tttagttaag atcctacttt ctagtcagat acgtaggtac tgggacctgg cacttaatat
     4981 gctcctatag ggctgactta tggagcgtcc aaggcaggac tccaccaagg aagctaactg
     5041 atgagttcca agaatcgcaa aagccagtca ggtttttcca tgatcgaact catggtcgtg
     5101 ctcatcgtga ttctcgttgt ggctggctac gccgtcccga acatacttag ctttatccac
     5161 atgaccaagc tccgaggctc cgcaagcgac tacgcctcga tcctgcaagt gggaagaatt
     5221 cgatcagtcc aagacaacaa gttttattca atttacctag tcgccggatc tccccaggaa
     5281 gcctacgtgg atctcacaaa caacggcggc acggtagcag ccccgggcga ccccttgatc
     5341 gcattatccg gcgaagtcgt tcctgtagct gcgggcggag cgccggacgc aaccgacctt
     5401 cagaacaagt tcttgccggc gggcgctccc gcgcctaatg acggaagcgc ggtgccagtg
     5461 attttcagtt cccacggcct tccctgcacc actcaagcgg ccactggagg gaatgtttgc
     5521 accggtgcct tggcgaccgc gttctgggtt ttcttccaag acacagtcac atcgagctgg
     5581 gaggcagtca ccgtaagccc ggccggccgc atccagaagt ggcaacacgg ttcgagtgat
     5641 tggaccaagc tctgaagtgc aacggtgatt aggacgaaag cataagcgat tcgcgaggca
     5701 catgtgagac ataaagcgga aagaggaatg accttaatcg agctgatgat tgccatggtg
     5761 gtcctggcca tcggtctcgg aggaatcacc actttgcttg tcacggctat tgcctcaaac
     5821 aacaaaaaca gcacagacac agcggctacg ctgcttgccc agatggtggt cgatcaaatc
     5881 agctctcaaa atatatacgc ccccaacaat gttctagtct ctgattgcgt cggcaatcaa
     5941 ctcgcgatca ccaccgcccc cggcgccgtg gggaccggaa ctggtgccac gctaaaagca
     6001 gacggaacga tcgacttcac acaggcctat tccgcgattc cagcaggcta tgccatgcgc
     6061 tacacggttt gttcaagctc agtaggcggc ataccaatga cctatgaagt tcgttggaac
     6121 gtaatgaagg tctcgacaaa tacgactacc cgcatgatca cagcggctgc acgtccgctg
     6181 gcttcgaatg caaaccaact cggcggattt tacttcgcct tgcccgtgaa tctgcggtcg
     6241 atcggcgcgc cgtcggcggg acaatagcga tggaagcgag ggttacaacc atgaatgccg
     6301 agcggcaggg tgggttctca ctaatagagc tgctagtttc gctggcgatt ctttctatcg
     6361 tgattggtgt tgtgctagat gggatcaata cgatgcaagc gagaaacacc gtggaaacca
     6421 ataagctgga tctcacccag cagacgcgcg agttcatgga tcagatcatc aacgatcttg
     6481 accaagccgg tttcccgagt ttgagcatgt tcgatccggt gggactcacg tcggcatccg
     6541 actgcacgca agacaataat gttgcttgcg gcttcaaata cctctcgacc agcgaaattc
     6601 agttcgaggg cgatgtggat ggcagtggcg tcagctatgt cgatatcaag ctcaatccgc
     6661 ccggggggcc ctgtccttgc acgattcagc gcggcgccat cgcaaagccc ggcaccactc
     6721 cgctctttta cactcaagtg gacaatgtaa tgaatacggc cgtatttaca gcctacgact
     6781 ctggtggcaa taacctcgac ttaactgctc ccatcacagg cgccggtggt ttcgacaaca
     6841 tcacggcgat cggaattacc ttgtacgtga aatcgtcgca gcctgacccc aagacgggac
     6901 agtatccgac tgtcacgatg gtctcgacag ccaaagtcaa caacattcag agctattgat
     6961 ccatagcact aaagccttga ggagggtctg accatgaaca acgcagccag gaccaaggaa
     7021 cgcggcatcg cgctgttctt tgccatcttc gcgctactct tgctcacggc aattggcgcg
     7081 gcgctcatct ttatgtcgag cacggaaacc tctgtgaact ccaactatcg ccaggagcag
     7141 gtggcttaca tgggagccaa ggcgggaatt gaagaagcca gggctcgcat gatggcatcg
     7201 gatccggctt ctatcgtgtg cggtgcgaca gtaggtccat gctcgcagcc cgtcatcggc
     7261 aacgcgagct acccgctctt tgactcaagc gcaatcaagc aggccactcc tccatcggcc
     7321 gccaaccaca tgatctatta catcttaaac ccaggaggcg ggagcgcggt gcaaccatgg
     7381 agcagcggaa ctcgctatgc ggatgacgag ctgtgccacg atggttattc gggcctcttc
     7441 tcagtctcgc aggtggtggc ccccgacgtc cggtgtagtg cgactccgtc tggcacggga
     7501 tggtacgatt cttacaccag cacccttccg ttcaatggca cgtccaaagc attgccttac
     7561 aagtgggtac gcatcgctcc caaactgaac ggttccatca gctatctcac tggcacaggt
     7621 tcaaacgtcg caacggtgcc gtactacgtc aaccaaactt ttagcggctc ggtagacgct
     7681 tttaccccga tttgctggga cggccacgag gaaaaggccc tgacgacgca aactgttccc
     7741 atagttatca attattgctc ccaaatgact agcaacgccg tgggcttcgc cggagcctac
     7801 atgacgaacg tttacacggt cacagcgctc ggggtcagtt cagatggtcc aaacgccgcc
     7861 cgaaaggtgg ttcaggctga cgtagccctg cagcccacca caccttttcc gtatgggtta
     7921 tacgcgacct cgacagcctg ccccgctata acgttcaccg gccagcaccc ctcgaccgat
     7981 agttacacca ctgcgggagg cggaacctat gcgaccacgc gatcgagcac cggcggagac
     8041 attggatcga atggtggggt tagtgtgcaa aatggaaaca ttggcggcat cgtaggcgtg
     8101 ctgcagccac cgccaaccgg gaatggaact tgcgcaaccc ctctgtcaat caacagtcca
     8161 ggcggtacga tggtcggcac cactacctgt ccgactggcg acaactcgaa tccgccagcg
     8221 tcgtgctatc tacccactcc ctacacattt cagactccgc ctcgcccaag tccccttcca
     8281 ccggtcacca actacacgcc cccgaattgc gcgggcggta agtgcatgac cccgggactc
     8341 tatggcaata tcaacgttaa cggcacgcta tacctcgcgc ccggcattta taacgtcaac
     8401 agcctttcta tgcagggaaa cggcgcgatc attgtaaatc cgcctggatc ggtaaccctt
     8461 aacgttgctg gctgcgcgaa ttccacatgt agcagcaccc tgggcaaccc cctctctatc
     8521 gccggtaacg gcataacgga cgatgcgata gccaatgatt ttacgatcaa ctatgctgga
     8581 actgggacag tcagcatcgc cggcaacggc aacgtcaccg ccatcctgaa tgctccgaac
     8641 gcccctgtca ctcaacaggg gaacggagcc tggtacggct cgatcctttc atctaccgca
     8701 tccattggtg gaaacgcttt cttccattac gacaagaacg ccgcgctagc ccccaacaac
     8761 aatggctact acacgttgat cggctatcgc gaggtcccct actgagatag ccgttggagc
     8821 cagagcaagc aacctgttac gtgtccccgg ttaaaggctc tgaaacgcct cgatcatcgg
     8881 cacccacggc ccgctccttt tcctcgggag cggccgtgtg cttggcgctg caatccaaga
     8941 gtcgatattt cgaacgcgca gtcagccgct ttcaggcgct gtcaccgggt tggtttcgct
     9001 gcacttcagc atagagcctc aatcgtcgga gccccctgaa aagtccacac aacaaccgct
     9061 tgcatccccg tctcccaccc tgtcaaccta gtcgcgatgt agtagcgtcc gccctctcga
     9121 cgaggccgga agttttgctc tttgacaact gaatcgcatg ctaaaacgag ctcacgcgtt
     9181 cggttagctc aatgccccct agacgagcgg tagctggcgg aggcgcaccg gccgttggac
     9241 attttgcttt agcagcgaga accggcgctt accggtcctt aacccgggct gccggcctag
     9301 gcgtggatca ttggcagtct aatcgacaca agagccgagt agaatatgtc gcatctgcta
     9361 gaaaacaaac ggctggggca gaagctactc gacacatctg tgggaaccca cgaaacaacc
     9421 taccggacta tgatccgcgg tggccagagt cttctccctt cttcggcttc cgccggactc
     9481 cgtcgtggcg ctgctcgtgt ttacttcgcc cgcgagtttc cctaccatag acctgcctcg
     9541 cgcaacttct tggagtgaat gcggcgcgca tctgcgcgcg ttaatggaca atgcctaacg
     9601 gccaaagact cgtcgtcgtc gaacccaacg gcacgcgccg cgatgtggtg gtgagcgcct
     9661 cgccgtttcg gattggccgc caagctggaa acgagttgac tctgcgcgac agccgagtca
     9721 gccgccatca ggcgcagata ttaaccgtcg aaggcaagct ggtgctggag gacatgggca
     9781 gcagccacgg cacttttgta aacggagaga aaattctccg ccatgaattg aagcccagcg
     9841 atcgaatcga cttcggcgtc gccgattcct accacgtgat ctacgttggc gacggcgcga
     9901 cgatcgaaga aatcgtcggg cgcatcgaag caccggctcc cgaacaatcg ggtccacgcg
     9961 aacttcatca tttgggcgtg ctgctcgagg tggcccgcgc tctcagctcg ggacttgcgc
    10021 tcgaagacat cctcacggcc gttttggacg ccgccatcgt cctcacccgt acggagcgcg
    10081 gcgtgctttt gctttacagc tcttcgggcg aggtacaaac agccgtcgcg cgcaatgcac
    10141 agggcgtcag cctgcgcacg gaagacgtcc aaatctcgca gggcgtgttg aagcgtgtcg
    10201 ccagcacgcg gcgtgaattg atcgtcagcg aaacggagga aggcagcgga ttaaccagcc
    10261 agcaggaaag catggcccgc ctcgctctcc gcaccatcgt ggccatcccc gtcgataagc
    10321 tgcccgtcat cgaagcgctc gacgctacga tctccgcgcg ccagggcgaa cttctcggaa
    10381 ttttgtatct cgatagccgc gctccttcga gcgccttttc cgcgctcgat cgcgaggtct
    10441 tgcgaacgct cgcgcgcgaa gccgccaccg tggtggaaaa cgcccgactt tttgcggcct
    10501 cgcgcgccaa agcgcgtctc gatcatgaaa tggaaattgc cagtcagatt cagcaaaacc
    10561 tgttgcctaa gacgttgccg aatctgccga acgttgcggt cgccggctcg accctcgcgt
    10621 gctattcggt cggcggtgat tgctttgacg tgatcgctct ggatggcggt cgccacggat
    10681 ttttcgtggg cgacgtttca ggaaaaggaa taaccgcggc actccttgcc acgttacttc
    10741 aaggcgtttt cttcaccacc gccgcgatgg atattccgct tcccggaatc ttttcgcgcg
    10801 tgaatcaata tctttgcgag cgtagcggag acgaccggta tgcaacagtt ttctacggcg
    10861 tcctggatag gacaggtcgc ttcgaatacg tcaatggagg
//
LOCUS       DATNDT010000210         4920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_45858, whole genome shotgun sequence.
ACCESSION   DATNDT010000210 DATNDT010000000
VERSION     DATNDT010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4920
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45858"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="VN788_06825"
     CDS             complement(<1..300)
                     /locus_tag="VN788_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20276.1"
                     /translation="MHTLLQRDQQPPVDSLIEMEPHSRLPRLDLGIDWESPRAEFISS
                     LRGVVSGPRAPKEAELTKERGFHVDWIRGRVPSKAFAASSLWHVAVIWLLILPIWG"
     gene            complement(343..906)
                     /gene="rsmD"
                     /locus_tag="VN788_06830"
     CDS             complement(343..906)
                     /gene="rsmD"
                     /locus_tag="VN788_06830"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HXU20277.1"
                     /translation="MRVIAGKFRSRKLKGPGLLPLRPTSDRLRETLFNILGPSIADSL
                     FVDLYAGTGAIGIEAISRGARQVIFVESNDKAAKLLRENLGALAIFPGAEFIEADAAK
                     ALEKLVKRRVLADFIFLDPPYESTDEHVATLDFLDASHLIAPQGMVIVEHSRKMPLIE
                     RLDRLERTRLVEQGDAALSFYRLAAAA"
     gene            complement(911..3010)
                     /gene="recG"
                     /locus_tag="VN788_06835"
     CDS             complement(911..3010)
                     /gene="recG"
                     /locus_tag="VN788_06835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HXU20278.1"
                     /translation="MTLANPVKYLRGVGPQRAAILEERGIGTVADLLSYLPFRYEDRM
                     HFTPIAEIVPGQVHTVLAEVASGNTVRFVRGRAPVFHLTVRDRSGALHARFFHGTYLQ
                     RLKEGQRLVLHGKADLDAYRPGRIEMVNPQIELVGSDQSAADSTEVGRIVPIYEAIGA
                     ISSRILRRIIYGVLLNFDGDLPDPLPEKLRERHRFPTRREAVLYAHFPPKNEDLELLN
                     SFRSPAQMRLIFEEFFYYQLALALRRQKENRQHGIAMRVREGKIREALKKILPFKPTA
                     SQKRVLSEIAADLERDYPMHRLLEGDVGSGKTIVAFEAATIVIENGCQVALMAPTEIL
                     AAQHFLSARRILEPSGYGIDLLVSGQKRPERGGVLARVESGETKLLVGTHALIEDPVT
                     FHRLGLAIVDEQHRFGVLQRKRLIEKGTAPHVLVMTATPIPRSLALTLYGDLDLSVID
                     EMPPGRTPIETRWTSDAQLPGVWEFLRREIGRGRQAFVLYPVIEESKQELKAATAEYE
                     RLAKTVFPNMRVGLLHGRLKNDEKDAVMERFRRRELDLLVATTVIEVGVDVPNATAMV
                     IEHADRFGLAQLHQLRGRIGRGSEKSYCILVAPKTITGDARERIETMVATSNGFEIAE
                     RDLKQRGPGEFFGTRQHGDAAFSFAQPLRDHELLELARSEAFALAENSEQAGKVVGSL
                     DSISPAWQKRYQLASVG"
     gene            complement(3061..>4920)
                     /locus_tag="VN788_06840"
     CDS             complement(3061..>4920)
                     /locus_tag="VN788_06840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU20279.1"
                     /translation="SLLAGAIAARAGQRGPAGESGEALYRQHCASCHDAGISRAPSRA
                     ALGQMSREGIRLALTSGSMAQQAANLSPAQIDALTHFLSNLDSSVAVAVAGTGACPAD
                     EARFSPRPDQPEWNGWGVDAEENRFQPAAMAQLRTADIPKLKLKWAFGFPYATQAYAE
                     PAVVGGRIFVGSAGPKIYSLDAKSGCTYWSSETEGPVRTAISVGARGNGWSAYFGDQH
                     GNVYAVDAETGKRLWKVRVDEHGSAQITGAPALVGDRLYVPVSSLEEVAGADAKDECC
                     KFRGSVSALDAVTGKVIWKSFTIAEEPKPVRKNSQGVQLWGPSGAGIWSSPAVDVKNH
                     MVYVTTGDNYSDPPTNTSDAFMAFDMDTGKMEWASQMHRGDAYNVNCDLPAAKQVNCP
                     AAKGPDFDFGSSPILVELPGGRRALVAGAKSGIVYAVDPDHRGKLLWQTRVGHGGTLG
                     GVQWGSAADTKNVYVALSDVQRRAAPPGSPAGHDSVFGVPYELDPNAGGGLFALSLDT
                     GSIVWHTPHPVCHKPGCSPAQSAAVTAIPGVIFSGGVDGHLRAYSAKDGKILWDVDTE
                     RDYQTVNGVKGHGGSLDGPGAVVVAGIVYVNSGYFFQGSAPGNVLLAFSVDGQ"
BASE COUNT         1000 a         1546 c         1353 g         1021 t
ORIGIN      
        1 cccccaaatg ggcaggatca acagccaaat caccgcaacg tgccacaaag atgacgcggc
       61 gaaagctttg ctcggaactc tgccgcgaat ccaatccaca tggaagccgc gctcttttgt
      121 aagttcggcc tctttcggcg cgcgcggtcc gctcaccaca ccacgcaggc tgctaatgaa
      181 ttccgctcgc ggggattccc agtcgatccc gaggtcgagg cgaggcagtc gcgaatgcgg
      241 ctccatctcg attaacgaat ccacgggcgg ctgctgatcg cgctgtagaa gagtgtgcat
      301 cggtccgagc attatacgtc gagaggattg gatgcgcgcc agctacgctg cggctgccag
      361 gcgatagaaa ctcagggcag catcaccctg ttcgaccaaa cgcgtgcgct caaggcgatc
      421 gagccgttcg atgagcggca ttttcctgct atgttcgacg atcaccatgc cctgaggcgc
      481 gatcagatgt gaggcatcga ggaagtcgag ggtggccaca tgttcgtcgg tgctctcgta
      541 cggcggatcg aggaaaatga aatcggcaag cacgcgccgc ttgacgagtt tttccaaagc
      601 tttcgccgca tccgcttcga tgaattcagc gccggggaaa atagcgagcg cacctagatt
      661 ctcgcgcagc aacttggccg ccttgtcatt ggattcgacg aaaattacct ggcgagctcc
      721 ccggctgatc gcctcgatac cgatcgcgcc ggtgcccgca tacaaatcca cgaaaagtga
      781 atccgcgatc gaaggaccga gaatattgaa aagcgtttcg cgcaagcgat cgctggtcgg
      841 ccgcaggggc agaagccctg gacccttcaa tttccggctg cgaaatttcc ccgctatcac
      901 gcgcatgtct tcatcccaca gacgccagtt ggtatcgctt ctgccaagcc gggctgatcg
      961 agtccaggct gccgaccact ttgccggcct gttccgaatt ttcggccaga gcgaatgcct
     1021 cgctgcgcgc cagttccagt agctcgtggt cgcgtaatgg ctgggcaaat gaaaatgccg
     1081 cgtcgccgtg ctggcgggtg ccaaaaaatt cacccggtcc ccgttgcttg aggtcgcgtt
     1141 cggctatttc gaagccgttc gacgtggcaa ccattgtctc gattcgctcg cgcgcatctc
     1201 ccgtgatcgt cttcggagcg acgaggatgc agtacgactt ttccgaacct cgtccgatac
     1261 gtccgcgaag ctgatgaagt tgtgcaagac cgaagcggtc ggcgtgctcg atcaccatcg
     1321 cagtcgcatt gggcacatcc acgccaacct caatcaccgt agtggccacc agaagatcca
     1381 attcgcgacg ccggaatcgc tccatcactg cgtctttttc gtcgtttttc agccgaccgt
     1441 gcagcaagcc cacgcgcatg tttgggaata ccgttttcgc caggcgctca tactccgccg
     1501 tggctgcttt caattcctgc ttcgattctt cgatcacagg atagagtacg aacgcttgcc
     1561 gcccgcgtcc gatttcacgc ctcagaaatt cccacacgcc cggcaattgg gcatccgaag
     1621 tccagcgagt ctcgatcggc gtgcgccccg gcggcatttc gtcgatcacg gaaagatcaa
     1681 gatcgccgta gagagttagc gccaggctgc gcggaatagg ggtcgcggtc atcaccagca
     1741 cgtgcggcgc agtgcccttc tcgattaggc gcttccgctg cagcacgcca aaacgatgct
     1801 gttcgtccac gatcgcaagc cccagccgat ggaatgtcac gggatcttcg atcagcgcgt
     1861 gcgtgccaac cagcaatttc gtttcgccgc tctcgacgcg cgccagcacg ccaccacgct
     1921 cagggcgctt ctggccgctc acgagcagat cgatgccgta tccggaaggt tccaaaatgc
     1981 gccgtgccga gagaaagtgt tgcgccgcca gaatttcagt gggagccatc agtgcgacct
     2041 ggcagccgtt ctcgattacg attgtcgcag cctcgaaggc cacaattgtt ttgccgctcc
     2101 caacgtcgcc ctctagcagc ctatgcatgg gatagtcacg ttccagatcg gcggcgattt
     2161 cgctcagcac tcgcttctgc gaggctgtcg gcttgaaggg aagaattttc tttagcgctt
     2221 cccgtatctt cccctcgcga acgcgcatcg caattccgtg ctggcgattt tctttttgtc
     2281 ggcgcagcgc aagcgcaagc tggtagtaga agaattcctc gaagatcaat cgcatctggg
     2341 cgggactgcg gaaactgttc agcagctcca gatcctcgtt cttcggcgga aaatgcgcgt
     2401 aaagcacggc ttcgcgccgc gtcgggaaac ggtgccgttc ccgcaatttc tcgggcagag
     2461 ggtcgggaag gtcgccatcg aaattcagca gcactccata aataattcgc cgcagaatcc
     2521 gcgagctgat tgcgccaatc gcttcataga tcggaacgat gcggcccact tctgtggaat
     2581 ccgctgcgct ctgatcgctg ccgacaagtt cgatctgtgg attcaccatt tcgatacgcc
     2641 ccggccggta cgcgtccaga tcggccttcc catgcaacac cagccgctgt ccctctttca
     2701 accgctgcag gtaggtgcca tgaaaaaaac gggcgtgaag cgcgccggaa cggtctctca
     2761 cggtcaggtg aaacaccggc gcgcggcctc ggacgaatcg aacggtattg ccgctagcga
     2821 cctccgcaag cactgtatga acctgcccgg gcacaatttc tgcgattggc gtgaaatgca
     2881 tgcggtcctc gtagcggaag ggcagatagc tcagcaggtc ggcaaccgtg ccaattccgc
     2941 gttcctccaa aatagcggct cgctgcggac caacccctcg caagtatttc accggattgg
     3001 caagtgtcac ggtcatcgca tggtcacacg aggaataatc ggattgcgca atgaatatgg
     3061 ctactggccg tccacggaaa atgccagcaa tacattgccg ggagcgcttc cttgaaagaa
     3121 atatccggaa ttaacgtaca caatccctgc tacgacgacc gcgccagggc catcgagcga
     3181 tcccccatgg cccttcactc cgttcaccgt ttggtagtcg cgctcggtat ccacatccca
     3241 gagaattttg ccgtctttag cggagtaagc tcgcaaatgg ccgtccacgc ccccagaaaa
     3301 aatcacgccg ggaatggcgg tcaccgccgc ggattgcgcc ggactgcatc ctggtttgtg
     3361 gcagacagga tgcggagtgt gccaaacaat acttcccgta tcgaggctaa gggcgaacaa
     3421 cccgccgcct gcattaggat ccagttcgta cggaacgcca aaaaccgaat catgtccggc
     3481 tggcgatcct ggcggtgcag cacgcctttg cacatccgac aaagcgacgt acacgttctt
     3541 cgtatccgca gccgagcccc actgcacgcc gcccagtgtc ccgccgtggc ctacgcgcgt
     3601 ttgccaaaga agcttgcccc gatgatcggg atcaaccgca tagacgattc ccgatttcgc
     3661 gcccgccacc agcgcacgcc ggccgccggg aagctccacc aaaataggcg acgatccgaa
     3721 gtcgaagtcc ggccccttgg ccgccggaca gtttacttgc ttcgcggcag ggagatcgca
     3781 attgacgttg taagcatcgc cccgatgcat ctggctcgcc cactccattt ttccagtatc
     3841 catgtcgaaa gccatgaatg catcggaagt gtttgtcggc gggtcggagt aattgtcgcc
     3901 ggttgtcacg tagaccatgt ggtttttaac gtccactgcc ggagaagacc agatgcctgc
     3961 gccggatggc ccccaaagtt gtactccctg cgaatttttt cggacaggct tgggctcttc
     4021 ggcgatcgtg aagcttttcc aaatcacttt gccggtcacg gcatccagcg ccgaaacgct
     4081 gcctctgaat ttgcagcatt catctttcgc atccgctccg gctacttcct ctagcgatga
     4141 caccggcacg taaagccggt ctcccacgag cgcaggcgca cctgtgatct gcgcggaccc
     4201 gtgttcatcc acgcgaacct tccagagacg tttgcccgtc tccgcgtcga ccgcgtacac
     4261 attgccgtgt tgatctccaa aatacgcgga ccagccgtta cctctggcgc ctacgctaat
     4321 cgctgtgcga accggcccct ctgtctcgct gctccaatat gtgcagccgc ttttcgcatc
     4381 aagggaataa atcttcgggc cggcgcttcc cacgaagatg cgccctccaa caacggcggg
     4441 ctcggcgtag gcttgcgtcg catacggaaa tccgaacgcc catttcaatt tgagcttcgg
     4501 aatatcggcc gtgcgtagtt gcgccatcgc tgcgggctgg aaccgatttt cctcagcgtc
     4561 aacaccccat ccattccatt cgggctggtc gggtctcgga gaaaatcggg cttcgtccgc
     4621 gggacatgct ccggttcccg cgactgcgac tgcgactgac gaatccagat ttgaaagaaa
     4681 atgtgttagg gcgtcgattt gcgcggggga cagatttgct gcttgctgcg ccatgcttcc
     4741 gctcgtcagc gccaagcgga tgccttcacg agacatctgc cccaacgccg cgcggctcgg
     4801 cgctcgtgat attcctgcgt cgtgacaact cgcgcagtgc tggcgataga gcgcctcgcc
     4861 gctttctccc gctggccctc gttgtcctgc gcgcgcagca atcgcgcctg ccaacaacga
//
LOCUS       DATNDT010000211         3259 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1111412, whole genome shotgun sequence.
ACCESSION   DATNDT010000211 DATNDT010000000
VERSION     DATNDT010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1111412"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1028)
                     /gene="hutI"
                     /locus_tag="VN788_06845"
     CDS             complement(<1..1028)
                     /gene="hutI"
                     /locus_tag="VN788_06845"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HXU20280.1"
                     /translation="MPDWLITGCSELLTLRGAAPRRGRDLSDLDIIRDGALLVRDDRT
                     VAAGPRRRIERLRAARRAEKLDLGGRVVLPGFVDSHTHLIFPASRADEYEQRISGKTY
                     EQIAKNGGGIRATVEALRRSRPEALVASASAHLREFAAHGTTTLEAKSGYGLNWMSEA
                     KILGILQQLHQDQPLDIVRTFLGAHIVPPEFHRRPDAFVDLLVKRWIPAVATAGLAEF
                     CDAFCDRGAFTVAQSRRILAAGRTCGLVPRIHAEQLAHIGAARLAIEVSAASADHLEK
                     IDASDIRALALSNVVCTLLPGCGFHLGTGCYAPARKLIDAGAIVALATDFNPGTSPTL
                     SMPMILSLA"
     gene            complement(1092..2780)
                     /gene="hutU"
                     /locus_tag="VN788_06850"
     CDS             complement(1092..2780)
                     /gene="hutU"
                     /locus_tag="VN788_06850"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013940947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HXU20281.1"
                     /translation="MVKLAVENPTGYQPIRAPRGTAISCKGWQQEAALRMLMNNLDPE
                     VAERPQDLIVYGGRGKAARNWECFHAIVASLKELESDQTLLVQSGKPVGIFRTHSYAP
                     RILIANSNLVGHWSDWQNFDELDRAGLMMYGQMTAGSWIYIGTQGILQGTYETFAAAG
                     RKHFGGDLAGKLVASGGMGGMGGAQPLAATLNGAAFLGIDVDPERIKRRVKSGYCDVM
                     VNDLDEALRILKNAVRKREATSVGLVGNCADLIPELARRGVVPDLLTDQTSAHDPIGG
                     YIPQELSVDQAAKIRKTDPESYRKRSLESIGMHVEGMLALQKLGSITFDYGNNIRTFA
                     FEQGVKNAYDFPGFVPAYIRPLFCEGRGPFRWVALSGEASDIHRTDKLVLEMFPQDEI
                     LCRWISLAQKRVRFQGLPARVCWLGYGQREKFGLALNDLVARGELKAPIVIGRDHLDC
                     GSVASPFRETEKMRDGSDAVADWPLLNALLNTASGASWVSIHNGGGVGIGYSLHAGQV
                     LVADGTPEMASRIERVLTNDPGIGVARHADAGYSGAIGFAGKSGIKIPMPGGKS"
BASE COUNT          662 a          941 c          990 g          666 t
ORIGIN      
        1 gccagcgaga gaatcatcgg catgctcagc gtgggactcg tgcccgggtt gaaatccgtc
       61 gctagcgcaa caatggcacc agcatcaatc agtttgcgcg ccggagcgta acaaccggtg
      121 cccaggtgaa agccacaacc cggaagcaag gtgcacacta cattcgagag tgcaagcgcg
      181 cgaatatcgc tcgcgtcgat tttttccaga tgatcggcgc tcgcggcgga aacttcgatt
      241 gccaggcgcg cggcgccaat gtgcgccagc tgctcggcgt gaatccgcgg caccaggccg
      301 caggttcggc ccgcggcaag gatgcgccgc gattgcgcta ccgtgaaggc gccgcggtcg
      361 caaaacgcgt cgcagaattc cgcaaggccg gcggtggcca ctgcgggaat ccatcgcttc
      421 accaacaaat ccacgaacgc gtccggccgg cggtggaatt ccggcggcac aatgtgggcg
      481 ccgagaaacg ttcgcacgat atcgagtggt tgatcctgat gcaattgctg gagaatccca
      541 aggatcttcg cttcgctcat ccaattgaga ccgtaaccgc tctttgcttc gagtgttgtg
      601 gtgccatgcg cggcgaattc acggagatga gcgctggcgc tagccacgag cgcttccggc
      661 cgtgagcgcc gcaaagcttc aacggtcgcg cgaatgccgc cgccattctt tgcgatttgc
      721 tcgtacgttt ttccggagat gcgttgctcg tattcatcgg cccggctcgc gggaaaaatt
      781 aggtgagtat gcgaatccac gaagccggga agaaccacgc gtccgccaag atcgagtttt
      841 tcggcgcggc gcgcggcgcg cagccgctcg atgcggcggc gcgggccagc ggcgacggtg
      901 cgatcatcac gaaccagcag cgcgccatcg cgaatgatgt ccagatcgga gaggtcgcgt
      961 ccgcggcgag gtgcagctcc gcgaagagta agcagttccg aacagccggt aatcagccaa
     1021 tcaggcacgg caattatcgg aatagtaact tattcgatag ctaaaagacc agaaatcgga
     1081 atgagcagcg atcacgattt accacccggc atcggaattt tgattccgga tttaccggcg
     1141 aagccgatgg ccccagagta gccggcgtcg gcatggcgcg cgacgccgat tcccggatca
     1201 ttcgtgagta ctcgctcgat gcgcgaggcc atttccggcg tgccgtccgc gaccaatacc
     1261 tgtcccgcat gcaatgaata gccgattccc accccgccgc cattgtgaat cgaaacccaa
     1321 cttgcaccgc tcgccgtatt gagtaaagcg ttgagcagcg gccagtccgc caccgcgtcg
     1381 gagccgtcgc gcattttttc agtttcgcga aaaggcgagg cgacggagcc gcaatcgaga
     1441 tgatcgcgtc cgatcacgat gggcgccttc agttcgccgc gcgcaactaa atcgttgagc
     1501 gccaagccga atttttcgcg ctggccatag cccagccaac agacgcgcgc gggcaatccc
     1561 tgaaagcgca cgcgcttctg cgcaaggctg atccagcggc agagaatttc gtcctgtgga
     1621 aacatttcga ggaccagttt atcggtgcga tgaatatcgg aagcctcgcc cgagagcgca
     1681 acccagcgaa acggtccgcg gccttcacag aatagcggac ggatgtaggc gggcacgaac
     1741 cccggaaaat cgtaggcgtt tttcacgcct tgctcgaagg cgaatgtccg gatgttgttt
     1801 ccgtaatcga aggtgatgga gccgagcttt tgcagcgcga gcatgccttc cacgtgcata
     1861 ccgatcgatt cgagggagcg tttgcggtag ctctcgggat cggttttgcg gatcttggcg
     1921 gcttgatcga cgctcagctc ttgaggaata tagccgccga tcgggtcgtg cgcgctggtt
     1981 tgatcggtta ggagatcggg gaccacgccg cgccgggcga gttcgggaat caagtccgcg
     2041 caattgccga ccaagccgac cgatgtggct tcgcgcttgc ggacggcatt tttcaagatg
     2101 cgtagcgcct cgtcgagatc gttaaccatc acatcgcagt agcccgattt gacgcggcgc
     2161 ttgatacgtt cgggatcgac gtcgatgccg agaaacgccg caccgttcag tgttgcggcg
     2221 agcggctgcg cgccgcccat tccgcccatg ccgccgctgg caactaattt tccggcgaga
     2281 tcgccgccga aatgtttgcg tccggccgct gcgaatgtct cataggtgcc ttgcagaatt
     2341 ccctgtgtgc cgatgtaaat ccagcttccg gcggtcatct ggccgtacat catcaggccg
     2401 gcgcgatcga gttcgtcgaa attttgccaa tcgctccagt gcccgactag attcgaattc
     2461 gcgatcagga tgcgcggggc gtaggagtga gtgcgaaata tccccactgg cttgccggat
     2521 tggaccagca gcgtctgatc gctttcaagt tctttgagcg atgcgacgat ggcgtggaag
     2581 cattcccaat tgcgggctgc tttgccgcgg ccaccgtaaa caatcaaatc ctggggcctt
     2641 tcagcaacct caggatcgag gttgttcatc agcatgcgca aggcggcctc ttgctgccat
     2701 cctttgcatg aaatggcggt gccgcgcggc gcgcgaattg gctggtagcc ggtcgggttc
     2761 tccacggcga gctttaccat gggcatgccc cttgggcgta tttagggtcg ttagttttgt
     2821 gtcgagtaga attggcaacg aagctttgtt ttgaataact tagaacacac tctattcgac
     2881 ccaactgtcg attaggctga cttgtctcag aggcgatata cccaagccag acgccgtcgc
     2941 cgagtgaggg accttcgccc tcacgaggac agtcctgcct ggcccttggt gagggccagc
     3001 actgcgcttc gaactcgatt cgagcgttca cgcgatcatc ttagtacgga attaagcggg
     3061 cgcaagggtc aggttctgtg gactaacaat gtgggcatct gggaaatgtg aatggccggg
     3121 cgcccgcaga gtggcaaatt gactttgccc ctgtgcggcg gtcgctcgac tatttgcaga
     3181 cgcgttctga catcgatatg cataaacttg gttaccttgg cgtcagcgtg ggttcggccg
     3241 aaggcgtcat ctataccac
//
LOCUS       DATNDT010000212         5883 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4116736, whole genome shotgun sequence.
ACCESSION   DATNDT010000212 DATNDT010000000
VERSION     DATNDT010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5883
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4116736"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..959)
                     /locus_tag="VN788_06855"
                     /pseudo
     CDS             complement(1..959)
                     /locus_tag="VN788_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014448370.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            complement(956..1351)
                     /locus_tag="VN788_06860"
     CDS             complement(956..1351)
                     /locus_tag="VN788_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU20282.1"
                     /translation="MEIKRIGSQASLKGTSEWFTGTVRIDPLFQAPDPALVQGARVTF
                     EPGARTAWHTHPLGQILIVTAGCGWAQREGKPIEEIRPGDVVWFSPGEKHWHGATPTT
                     AMTHIAIQEKLDGKVVDWMEQVSDEQYKR"
     gene            complement(1424..2407)
                     /locus_tag="VN788_06865"
     CDS             complement(1424..2407)
                     /locus_tag="VN788_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXU20283.1"
                     /translation="MQKRKLGKSGLEVSAIGLGCMGLSFGYGPAVEKEQAITLIRSAF
                     DYGVTFFDTAEAYGPFTNEEVVGEALAPFREQVAIATKFGFEDGDSHKGLNSRPERIR
                     KVAEAALKRLQTDVIDLFYQHRVDPNVPIEDVAGTVKDLLQEGKVKHFGLSEAGAQTI
                     RRAHAVQPVTALQSEYSLWWREPEQEVLPTLEELGIGFVPFSPLGKGFLTGAITENTT
                     FDSSDFRNIVPRFTPEARRANQTLVGLLDGIAERKNATPAQIALAWLLAQKPWIVPIP
                     GTTKLHRLEENIGAAEVELKAGDLRDIDSAVSKITVEGARYPAYLQQRVDR"
     gene            2544..3521
                     /locus_tag="VN788_06870"
     CDS             2544..3521
                     /locus_tag="VN788_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HXU20284.1"
                     /translation="MRRTKTSPVKNPSNQASPRHDRARDLRTELARKVVLFIGSEENL
                     ATAIPSLTLHRRTAPTAPCPATYEPSVTVIAQGRKQVDLGQTTFIYDESRFLLTSIDL
                     PIISRVVEASEEVPCLALVLKLDMSMVREFLSREEIHMLDVPSDSPAMSTGETTVEFL
                     SACCRLMDLLQTPRDIPFLGGLIEREITYRILCGPQGARLRAIATLGDQSHRTAKAIA
                     WIRANYAKPLRVEELAQIAGMGGSTLYHHFRALTAMSPLQYQKQVRLQAARARMLAYD
                     VDAATVAFEVGYESASQFNREYSRFFGQPPMRDIRSLRSLAERSAVRID"
     gene            3762..>5883
                     /locus_tag="VN788_06875"
     CDS             3762..>5883
                     /locus_tag="VN788_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU20285.1"
                     /translation="MLIHILTKLHNGNIKLSWLAALLVIFSISAVAQNRNAGEIRGTV
                     FDQTGAAVPAATVTILNKQTGITQTLTAAGNGVYDAPSVEPGTYDITFSKEGFRKLVR
                     GNVVLHVEAITIDAQLQIGSESQQITVESGAPLVQTETSDRQMILTTEAVTQLPNIGG
                     SWYDFTGQLPGVNPGKGGQDASGEGVGVNGTTSYLSNWLIDGGVGTFPVSQNPDFAKP
                     PLDAVSELNFNTSNFAAEYGSGVAVFNVITKSGTNAFHGSAYEFVQNDIFEARNFFAE
                     KVTPLRWNQYGGSVGGPIKRDKLFFFFSYQRNPINNFSPTFYTFPTTAMRNGDFSAPG
                     LPVIYDPTSGTGQNPRTPYPGNQIPAAQIDPVAQKIQSYLPQPNRPGIANNYYFAGSN
                     PITWTTYDGKLDFNLTASNRLTGSGLWVSQTNITPAPTCPMDCNNNDQYEYQAQVTDV
                     WTLSPSIVNEARVSTIRTYGTWFSPNQGQGYPAKLGLLNPAADAFPGITVGGTVGTSI
                     SGGLSAVLGFTSYVTSDTVTLVKGKHIIKFGGELDKWQDNQAWDNKRSGNFDFNGIFT
                     RNPADPTSSGLGYADFLIGLPDTWSVSNAPETGMRNWNVQAFAQDDYKIKRNLTLNFG
                     VRYQLQTGWVEEHNRLASFDPSITNPATGTPGALWFAGQNGRNALQKTIPNFFAPRLG
                     FAWSPRPSWSVRGGFGMFANMWGAN"
BASE COUNT         1269 a         1705 c         1630 g         1279 t
ORIGIN      
        1 cgatatttca cgtcggactt aagcagcctg tcgtaggctt cgttagcctt ctggatcggg
       61 atgacctcga cgtccgcggt gatgttatgc tctctgcaaa agtcgagcat ctcctgagtc
      121 tcgggaatac cgccgatgtt cgaaccagaa acgctgcggc atcccaaaat aagtcccaac
      181 gacgagacgt ccagaggctt cgccgaacgc cgacaagagt aagagcaggt tgatataggc
      241 gttgatgccg tgatccgccg atacggcgtc acgaatgaaa tcgaagctgc cggcgtgctt
      301 tttatctcgt gcgcattccg ggaaaggacg atttcgccgg cgccgagacg aagcgcgtcc
      361 tccttttgct gggcgaagtg gtgaagttca caacatgagt tccaagcgca tgtgcaaact
      421 tcacggccat gtgaccccgc cggccgagcc cggcgacgcc aacctttttg tccttcgtaa
      481 tgccccagtg gcgcatgggc gagtaggttg tgatgccggc gcagagcagc ggcgcggccc
      541 ggcgagatcc agattggagg gcactcgcag aacgaaaacg ctcattgatg tcgacactat
      601 cggagtagtt gaaggtgagc gtcagattcg ggcagaaatt ctcgaggcct gccttgcatc
      661 ttgggtaggt gcggtcgaag tcgatcaggc agccgactgc ggcgaggtcg cccggcttga
      721 acttggttac tgccgagccg gccttcgtga cgcgtccgac gattcatggc cgggcacaat
      781 cgggtagcta gtcggtatga actcgctcca ctcgttgcgg accgaatgga gatcggagtg
      841 gcagatgccg caaaaaagat ttcgatctgc acgccctgtt cggttggatc gcgccgcgcg
      901 attgtcgtag aggcaagccg cgacgtcgtt ctgcgagttt ccttcgaccc ctcagtcagc
      961 gtttgtattg ttcgtcgctg acctgttcca tccagtcgac aaccttccca tcgagctttt
     1021 cctgaatggc gatgtgcgtc atcgccgtag ttggcgtggc gccatgccag tgcttttcgc
     1081 cgggcgagaa ccagaccaca tcacccggcc ggatctcctc gattggtttg ccctcgcgtt
     1141 gagcccagcc gcagcccgcc gtcacaataa ggatctgacc aagcggatgc gtatgccagg
     1201 cggtccgcgc gcccggctcg aaagttacgc gggcgccttg aaccagtgcc ggatcaggcg
     1261 cctgaaaaag cggatcgatc cgtaccgtgc cagtgaacca ctctgatgtc cccttgagcg
     1321 aagcttgcga gccaattctt ttgatctcca tctctaaact cctctactta aggttgtcac
     1381 gtagtcgtac gcaatcagaa ctgttccacg caagtcttgc tgctcagcga tcgacccgct
     1441 gctgcagata cgccggatat cgtgcgcctt ccaccgtgat cttggagacg gcgctgtcga
     1501 tgtcgcggag atcgccggcc ttcagttcaa cttcagccgc tccgatattc tcctccaggc
     1561 gatgcagctt cgtggttccg gggatcggaa cgatccacgg tttctgagcc aacagccagg
     1621 cgagcgcgat ctgagcaggc gtggcattct tgcgctcagc gatcccgtca agcaaaccga
     1681 ccagagtctg gttcgccctc cgagcctccg gcgtgaaccg gggaacgatg ttgcggaaat
     1741 cggagctgtc gaatgtcgtg ttctcggtga ttgcccctgt aagaaagccc ttacccagag
     1801 gactgaaggg aacaaaacca atgccaagtt cctccagagt tggtagaacc tcctgctccg
     1861 gctcccgcca ccacagcgaa tactcgcttt ggagagcggt aacaggctga acggcgtgcg
     1921 cgcgacggat cgtctgcgcg cctgcttcgg agaggccgaa gtgcttgacc ttgccttcct
     1981 ggaggagatc cttcaccgta ccggcgacgt cctcgatcgg aacgttcgga tcaacgcgat
     2041 gctggtagaa cagatcaatg acatcggtct gtagccgctt cagcgctgcc tcggcgactt
     2101 tcctgatgcg ctccggccgg ctgttcagtc ccttatgcga gtctccgtct tcgaagccga
     2161 atttagttgc gatcgccacc tgttcgcgga agggcgcaag ggcctcgcct accacttcct
     2221 cgttggtaaa cgggccataa gcttcggcgg tatcaaagaa tgtcacgcca taatcgaatg
     2281 ccgacctgat cagcgtgatt gcctgctcct tctcaactgc ggggccgtag ccaaagctca
     2341 gtcccatgca gcctagtccg atggctgaga cttcgaggcc gctctttcca agtttgcgtt
     2401 tctgcattag ttctcgtcat cctctatttc caggctagga gaggcacgct tcgacgggta
     2461 tcctgatcct gccgaattgt tgcctattcc tgtcgaagat tgccgtaacc gttctacatc
     2521 gtaacgccca ggcggctatc ctgatgagga gaacgaaaac atcacccgtg aaaaatccca
     2581 gcaatcaggc gtctccgcgg catgatcgag cacgtgactt gcggactgag ctggctcgca
     2641 aggttgtcct gttcattggc tcggaagaga acctggcaac ggcgattcct tccttaacgc
     2701 tgcatcggcg gaccgctccc accgccccgt gcccggcgac gtacgagccc agtgtgacgg
     2761 tgatcgctca aggtcgaaag caggtcgacc tgggtcaaac gactttcatt tacgatgagt
     2821 cgcgattcct gctgacgtcg atcgatttgc cgatcataag tagggtggtc gaggcaagcg
     2881 aagaagtgcc ctgtctcgcc ttagtactca agctcgacat gtccatggtt cgcgaatttc
     2941 tcagccggga ggaaattcac atgctcgacg ttccctctga cagtcctgcg atgtccactg
     3001 gtgagaccac ggtggaattc ctcagtgcct gctgccggct gatggacctg ctacaaacgc
     3061 cgcgggacat tccctttctc ggcggcctga ttgaacgcga gattacctac cgaatcctgt
     3121 gcggaccaca gggagcacgg cttcgagcga tcgccacgct gggagaccag agccatcgga
     3181 ccgcaaaagc gattgcctgg atcagggcaa actatgccaa gccgcttcgg gtggaagagc
     3241 tcgcgcaaat cgcgggcatg ggagggtcca cgctgtacca tcacttccgg gcgctgaccg
     3301 ccatgagccc gcttcagtat cagaagcaag ttcggttgca ggcggcgcgg gcacgcatgc
     3361 tcgcgtatga tgtggatgcg gcgacggtgg cctttgaggt cggatacgaa agtgccagtc
     3421 aattcaatcg ggaatacagc cgtttcttcg gccaaccacc gatgcgggac attcgtagtc
     3481 ttcgctcgct cgctgagcgc tctgccgtca gaatcgatta gttaatcaag gtcggcaatc
     3541 caaacgctgt gtgatacgtt tgctaacggg cttgccgtgc ctcgggataa aagagcttgg
     3601 cgccggcagt ttccctgagc ggtgttttct ccagatgcgg cgtgatccag ccaacattct
     3661 caattctgcc cttgacaggc ttaattactg ggcatagagt tgccgttaac gagattccag
     3721 gcgaattacg ctcgaaaagc ctgctcgcta aggagaccct cttgttaatt catatcctca
     3781 cgaaactcca caacggcaat ataaagctta gttggctggc ggcattgctc gtaatattct
     3841 cgatttccgc tgtcgcccag aaccggaacg cgggcgagat acgcggcacg gttttcgacc
     3901 aaacaggcgc ggccgttcca gccgccacgg ttacaatact gaacaaacag actggcatca
     3961 cgcagactct caccgcggcg gggaatggtg tttatgacgc tccgtccgtc gagcccggca
     4021 cgtacgacat caccttctcc aaagagggat tccggaagct tgtccgcggc aacgtcgtgc
     4081 ttcatgtgga ggcaatcacc atcgatgcac aattgcagat cgggtcggaa tcgcaacaga
     4141 taacggtgga atctggtgcg ccattggtcc agaccgaaac gtcggatcgc cagatgattc
     4201 ttacaacgga agcagtgaca caactgccca atatcggcgg cagttggtac gactttacag
     4261 gccaattgcc aggcgtgaac cccggcaaag gcggccagga cgcgtccggt gaaggcgtcg
     4321 gcgtgaacgg aaccacctct tacctttcga actggttgat tgacggtggc gtcggcactt
     4381 tcccggtaag ccagaatccc gacttcgcaa aaccgcctct tgacgctgtc tccgaattga
     4441 atttcaatac cagtaatttc gcggcagagt acggcagcgg cgtcgccgtc tttaacgtaa
     4501 tcacgaaaag tggcacaaac gcctttcacg gctcggctta tgagtttgtt cagaacgata
     4561 tattcgaggc aagaaacttc ttcgccgaaa aagtaactcc cctgcgatgg aatcagtacg
     4621 gcggcagcgt cggcggacca attaagcggg acaagctttt ctttttcttt agctaccagc
     4681 gcaatccgat aaacaatttt tctccaacgt tctacacatt tcccacaacg gccatgcgca
     4741 acggagattt ttcggcgccc ggattgccgg tgatttacga tccaacgtcg ggaacagggc
     4801 agaatccccg gactccatac cccggcaatc agattcccgc cgcgcaaata gatccggttg
     4861 cgcagaaaat ccaatcctat ctgcctcagc cgaaccgccc cggcatcgcg aataactact
     4921 acttcgcagg ctctaacccg ataacctgga cgacttacga tggaaagctc gattttaatc
     4981 ttacggcgag taacaggctg actggctcgg gcctttgggt aagccagaca aatatcacgc
     5041 cggctcccac ttgccccatg gattgcaata ataacgatca gtatgagtat caggcgcagg
     5101 tgaccgatgt gtggacgctg agcccctcga ttgtgaacga ggctcgtgtt tcaaccattc
     5161 ggacgtacgg tacctggttc tcgcccaatc aaggccaagg ttatccagcc aaactgggcc
     5221 tgttgaatcc cgcggctgac gcttttcccg gcatcaccgt cggaggtacc gtcggcacgt
     5281 cgatcagtgg cggcctttcc gccgtgctcg gattcactag ttatgtaaca tccgatacgg
     5341 tcactttggt aaagggcaaa cacattatca aattcggcgg cgagcttgat aaatggcagg
     5401 acaatcaggc ttgggataat aaacgttcgg gcaacttcga tttcaacggc attttcaccc
     5461 gtaatccggc cgacccaacc agctcgggct tgggctatgc ggactttctg atcggccttc
     5521 ccgatacgtg gtccgtcagc aatgctcccg aaaccggaat gcggaattgg aacgtgcagg
     5581 cattcgcaca agacgattac aagatcaagc ggaatttaac tctcaacttt ggagtgcgct
     5641 atcagttgca gaccggatgg gtggaagagc acaatcggct agccagcttc gatccttcca
     5701 tcaccaaccc agcaaccgga acacccggcg cgctatggtt cgccggacag aacggacgga
     5761 acgcgctcca aaagaccatt ccgaatttct ttgcgccgag gctcggcttc gcttggtcgc
     5821 ctcgccctag ctggtccgtg cgtggcggct ttggaatgtt cgcgaatatg tggggcgcga
     5881 aca
//
LOCUS       DATNDT010000213        10359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3229931, whole genome shotgun sequence.
ACCESSION   DATNDT010000213 DATNDT010000000
VERSION     DATNDT010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10359
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3229931"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..604)
                     /locus_tag="VN788_06880"
     CDS             complement(<1..604)
                     /locus_tag="VN788_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20286.1"
                     /translation="MTARFAPGTPPHLAIRADYQLKNVGTTPLDSIEVAVPGEKDFGR
                     SNLRVLVASREITAQSEQGANSESATGESAAAESLWRIPLAPALRKKQKINVSLEYDL
                     AATEATDPRIFAAENMFYLNDSGWFPEFQEPKALFAEDITRPNPTDLTVIVPANFLVT
                     ASGQPHGEKKTRAETEHHFRITAGDFDPYVLAGAYQQQQMA"
     gene            complement(1085..1729)
                     /locus_tag="VN788_06885"
     CDS             complement(1085..1729)
                     /locus_tag="VN788_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20287.1"
                     /translation="MPAAPFSLNPAALLRRSRLLVLFCSASFALAACQRLKPLDTSVL
                     DQSGMSFATVQTLRGMNITNAEVDEIAKARQAGLSDEDCLTLFRLERSRKQDFDIGDT
                     VAHLLRAGSSGSTVVQLAQIDQLHSWGGEAVAMRLAGLSDQIILEDAHRRASDLPVLS
                     GASLANLKNTGMREATLLQLVRRGVPDDEADTIISLRRRGWSDAMVLRRYPGGR"
     gene            1854..2564
                     /locus_tag="VN788_06890"
     CDS             1854..2564
                     /locus_tag="VN788_06890"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXU20288.1"
                     /translation="MNMATGSVSPRAANAREEQGFAARLRGFGPVGLLGIAVILAGAM
                     VNGLVSAALVVLWVVLSKTSWREIGYVRPKNYALTIAGGMAFGAALKLLLKAAVMPLL
                     GASPVNRSYHFLAHNSAALPGFLLLVVVSAGWGEETFWRGYLFERVRRIFGWGAATRI
                     TVVLLTSVLFGMAHYQGQGRDGVMQAIITGLTFGAIFAYTGRLAFVMIAHAAFDVVAV
                     LIIYFDIETHIAHLIFRF"
     gene            complement(2716..4953)
                     /locus_tag="VN788_06895"
     CDS             complement(2716..4953)
                     /locus_tag="VN788_06895"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HXU20289.1"
                     /translation="MSPERKPAGDPVAQRVLDLKFVELMDKVRRNRPGDDLDLLRRAY
                     DFAAEQHRSQRRESGDLYLSHPLAVAGILADMKLDVTTLCAALLHDVVEDTRIPIDRI
                     SEMFGADVARVVGGATKISRIDLLAPEARQAENVRKMLLAMVSDVRVILVKLADRLHN
                     MRTLEFLDPERQRRIATETLEIYAPVANRLGMGVMRGELEDLAFRFLEPAAFLELQKK
                     VVTKHKLQEKFLHEVQDAIRTRMVESGIPAEVEGRTKGLFSLHQKMLRQQRSIDQIYD
                     LMAVRVVTDTVKNCYAALGVVHQIWRPVPGRFKDYISMPRPNLYQSLHTTVLHSGQPF
                     EIQIRTQEMHRIAEQGVAAHWKYKDARPPSGRPGADGRVDVDDQRDDQRVVWMRQLIE
                     WVQEMEEPSEFLSTLKVDLYPEEVYTFSPKGRVVVLSRGATPVDFAYAIHTEVGHQCV
                     GAKVNGQIVPLRHVLANGDVVEIITQKNHGPSRDWLKFVQTSRSRSKIRHWINLHEHE
                     EATTVGRRLLEREARHSGISLKKIADEDWQRVASEYGCGHIDDLHADLGYGKWSARQV
                     LAKASNQPLVESTEEKAPKIVSTVKRMLGMQNAPIQVRGHDDLMVYRAKCCNPIPGDD
                     IVGYVTRGRGVAVHSTSCPNVKNLLYETERRIAAEWAGTAESTFSVRLRILTEDRPGM
                     LAEITAAISVADANIRTLESIGEHLHARIEIALDVRDRKQLEKILAQIKKTPGIFDIE
                     RVYNV"
     gene            complement(5068..5892)
                     /locus_tag="VN788_06900"
     CDS             complement(5068..5892)
                     /locus_tag="VN788_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU20290.1"
                     /translation="MNRKPAAILSASVLLLALGAAGCAKLKARDDLNKGVQAYNNAQF
                     EQAIEYFKDATQNDPKLTNAQLFLAQAYAQQYIPGAPSEENKRNGEQAIAVWKKVLES
                     NPNNTTALTGIGSMYYNMAGSPFNRDMMEQAKDYQEKALALNPNDATPYYWVGVIDYW
                     IAFRTNAQLRADYNQKAKKPLRPLDPLPAKVRDDFVQKEGDTVKEGIDNLNHALQIKP
                     DYANAMAYLNLLLREKADMESDPAARDADEKQADQLLDKVKEINQKQMTTQPGSTH"
     gene            complement(6003..6395)
                     /locus_tag="VN788_06905"
     CDS             complement(6003..6395)
                     /locus_tag="VN788_06905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU20291.1"
                     /translation="MSEPNVVPLIDVLLVLIIIFMAITPTTPTGMAADVPQPPPKKQQ
                     QQQQNDTTIVVVISSDGKVSINQTPTSWAELGPQLDNIFKIRAQKIAFVKGDDDTQFA
                     YVAHAIDIMHGVGITDVGLITAKMEAGQ"
     gene            complement(6466..6891)
                     /locus_tag="VN788_06910"
     CDS             complement(6466..6891)
                     /locus_tag="VN788_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU20292.1"
                     /translation="MGISVGGHGSSMSEPNVVPLIDVLLVLIIIFMAITPTTPTGMAA
                     DVPQPPPKTQKPPTQNDTIVVVVSSDGKVSINQTPTTWAELGPQLDNIFKIRAQKIAF
                     VKGDDDTAFAYVAHAIDIMHGVGITDVGMITAKMEAAGG"
     gene            complement(6909..7364)
                     /locus_tag="VN788_06915"
     CDS             complement(6909..7364)
                     /locus_tag="VN788_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU20293.1"
                     /translation="MPYKPKVGATMAAPNVIPMADIMLVLLIIMMVITPMLNNHQLVD
                     MAKAKNWQNMPQAERDDAVVIAVTSDSRFYLGNNEIALNQITSTVQDKMSNRLDKTVY
                     LRSDERAKYGDVKKAIDAIRDAGIENVGLLTLKDESNQPPGPPNLTPGS"
     gene            complement(7368..8108)
                     /locus_tag="VN788_06920"
     CDS             complement(7368..8108)
                     /locus_tag="VN788_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXU20294.1"
                     /translation="MLSNLVWAAFLFLQDQGGSAGAPGWDIRSMWHTMDIPAKVVVII
                     LFIMSAWSVGIMIDRALMYSAARKQSRVFVQQVAGALKDNKLDEAISIAERNKKSHIA
                     KVVATGLSEFQAASAQVADEEVIEAAKRGLERSVAIVHAEMKRGLSGLATIGATAPFV
                     GLFGTVMGIIGAFKGIAAQHASGLSAVAGGIAEALVTTALGLLVAVPAVWTYNYFTNK
                     VEAFDVEMDNSSMELVNYFITRRAGARK"
     gene            complement(8168..8884)
                     /locus_tag="VN788_06925"
     CDS             complement(8168..8884)
                     /locus_tag="VN788_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXU20295.1"
                     /translation="MFDQLVESTNKKATAKVWSVWVSAGIQALILIVLILIPLIYTRA
                     LPPLLSTSFLAAPPPPPPPPPPAAVVKVKPQPKFEQKMVTPTVVPKKIAMIHQEAPPP
                     DVGVTGGVEGGVPGGSAGGVLGGIIGGAPGGLPAPPPKPTAPIRVGGNVMQGKILREV
                     KPEYPQIARMTHQQGTVVLHAIIDEHGDITSLTYISGPALLKQAALDAVRQWKYAPYQ
                     LNGTPVKVDTTISVVFTLGE"
     gene            complement(8890..10194)
                     /gene="secF"
                     /locus_tag="VN788_06930"
     CDS             complement(8890..10194)
                     /gene="secF"
                     /locus_tag="VN788_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HXU20296.1"
                     /translation="MEFFHAPNFDWMKKTKYFVTLSLCLLAIGAAAAIYHHGLHYGID
                     FEGGTQVIVRFSSAPPISQIRTALDNVGLKNNEVTSSSDISNPNSRNDVMIRISQVAK
                     GEDEDNAAKAAILSALEKNFGAPSSSKPDFNAVSTSDLANYLTKRDPLMLQTAAGDRY
                     TQLAQSLVNARDREHGGIINNFDQLSNVAGANAAVIAALKAGYSLNSFSILNAQVVGP
                     TVGAQLRRQAILATLYALAGMLIYVAIRFSGGDLLHFSNAGGFFLWRWIDYTIFGLAA
                     VIAVFHDVLITLGFFCLFYGKFEISLTVIAALLTLVGYSMNDTIVIFDRVRENLRLMR
                     REPLNVIVNRSINQTLSRTILTSGLTFLTVFLLFVFGGQVLRSFAFAMVIGVVVGTYS
                     SFGIAAPIVVVWNKWRGQRGATISVPSSADKRAEKRLATARR"
BASE COUNT         2091 a         2718 c         3286 g         2264 t
ORIGIN      
        1 cggccatttg ctgctgctga tacgcgccgg cgaggacgta cggatcaaaa tcgccggcgg
       61 tgatgcggaa atgatgctcg gtttctgcgc gcgtcttttt ttcgccgtgc ggctggccgc
      121 tcgctgtgac gaggaaattt gcaggaacga tcacggttag atcagtggga ttcgggcggg
      181 tgatgtcctc ggcgaagaga gctttgggct cctgaaattc agggaaccag ccggagtcgt
      241 tcaggtaaaa catattttca gcggcaaaaa tgcgcgggtc ggttgcctcc gtggcggcga
      301 gatcgtattc gaggctcaca ttgattttct gttttttgcg gagcgcgggc gctaacggaa
      361 tgcgccacaa ggattctgct gccgcggatt cgccggttgc agattcggaa tttgcaccct
      421 gttcggactg cgccgtgatt tcgcggctcg cgacgaggac acggagattt gagcggccga
      481 aatctttttc gcccggcaca gcgacttcga tggaatcgag aggagttgtt ccgacgttct
      541 tgagctgata atcggcgcga atggcgaggt gcggcggcgt tccgggggca aagcgggcgg
      601 tcaaggattc tttttcgact tgatagccgg gagcgagcgg gacggcgcac ccggcgagga
      661 gagttgcaaa ggacaatgcg aggaaaatgg tggcgtgcgc gcgatgcgag gtggatgcga
      721 acaaagcgta gagattttac cgagcgggcg cactggggtc tagcgttgtg aatgcgattc
      781 aaaggttacg ggcaaggcgc catgtgagga cgtgttgcta ccaaaaaaca aaatgcagat
      841 tcctcgggcg aaaagcaccc ttcggaatga tagtacccgg ttatttttcg gccttaggat
      901 gcttttgaag gacactgcag attttcccca ccaaggagag agccttcgaa tgacagtgtc
      961 tttttccttt gagccccagg gacgactata gatagaagga acttggggat ttgcgacagg
     1021 gccgacgagg tcgtcgatgc tggtcgcggc ctcacgcgcg acggcaatct ttaatcttga
     1081 tcagctagcg gccaccggga tagcggcgga ggaccatggc gtccgaccag ccgcgccggc
     1141 gaagggagat gatggtgtct gcttcatcgt cgggcacgcc gcggcggacg agctggagca
     1201 aagtggcttc gcgcatcccc gtatttttca ggttggccag cgatgcgccg gagagcaccg
     1261 gaagatcgga ggcgcggcgg tgcgcgtcct cgagaataat ttggtcggag aggccggcaa
     1321 gtcgcatggc cacggcttcg cctccccaag agtggagttg gtcgatttgc gcaagctgca
     1381 caacggtcga gccggaagat ccggcgcgga gaaggtgcgc gaccgtgtcg ccgatgtcga
     1441 aatcctgctt gcggctgcgt tcgaggcgaa atagcgtgag gcaatcttcg tcggagaggc
     1501 cggcctggcg cgctttggcg atttcgtcca cttccgcgtt ggtgatgttc atgccgcgaa
     1561 gagtctgaac ggtggcgaag ctcattccgg actgatcgag cacgctggta tcgaggggct
     1621 tgaggcgctg gcaggccgcg agagcgaacg acgcgctgca gaacaaaacg agcaggcgcg
     1681 aacgccgcag cagagcggct ggattcagcg aaaaaggtgc agcgggcata acagagcgat
     1741 tttagcgtga ggcgacgcac gagaagggcg cgtatgtcgg aaacgtcaat ggaagatagg
     1801 aaacgaacgg tcagagcttg gctaaccatc tcactggatt gttataagta atcatgaata
     1861 tggctacagg ttcagtatcg cctcgcgcgg caaacgccag ggaggagcag ggctttgcgg
     1921 cgaggctgcg cggttttggg ccggtgggcc tgctggggat tgctgtgatt ctggcgggcg
     1981 ccatggtcaa cgggctggtc tcggcggcgc tggtggtgct gtgggtcgtg ctctcgaaaa
     2041 catcgtggcg cgaaatcggc tatgtgcggc ctaaaaacta tgcgctgacg attgcgggtg
     2101 gcatggcatt cggcgccgcc ttgaaacttt tattgaaggc agccgtcatg ccgctgctgg
     2161 gggccagtcc cgttaatcgc tcctatcatt tcctcgcgca caattcggcg gctcttcccg
     2221 gatttctgct gttggttgtg gtgagcgcgg gatggggaga agaaactttc tggcgcggct
     2281 atttattcga gcgagtgagg cgcatttttg gctggggcgc tgcgacgagg atcactgttg
     2341 tgctgctgac gtcagtactg ttcggaatgg ctcattacca gggacagggc cgcgatggag
     2401 tgatgcaggc aatcatcacc ggcttgactt tcggcgcaat cttcgcgtac acgggccggc
     2461 tggcgtttgt gatgatcgcg catgcggcat tcgatgtggt ggcggtgctg attatttatt
     2521 tcgatattga gacgcacatt gcgcacctga tcttcagatt ttgaatttac ccggtctggc
     2581 tgccgacttt gcggcacgcc cactgaaatc cagcacgttt ctaccaaacg gaattgaagc
     2641 gccagccgtc tgtctgcgac gtctacctgc gaaatatgag aatcgcgtga ggcggaaatg
     2701 aaactggtat gactgttaga cgttgtagac gcgttcgatg tcaaagatgc cgggagtttt
     2761 cttgatttgc gcgaggattt tttcgagctg cttgcggtcg cgcacatcga gggcgatttc
     2821 gatgcgggcg tgcaaatgtt cgccaatact ttcgagcgtg cggatgttcg cgtcggcgac
     2881 gctaatcgcc gcggtgattt cggcaagcat cccggggcga tcttcagtga ggatgcggag
     2941 gcggacggag aaagtggatt cggcggttcc ggcccattcg gcggcgatgc ggcgctcggt
     3001 ttcgtagagc agattcttga cgttggggca cgacgtggag tgcacagcga cgccgcggcc
     3061 gcgcgtgacg tagccgacga tgtcgtcgcc gggaatcgga ttgcagcatt tggcgcgata
     3121 gaccatcagg tcatcatgtc cgcgaacctg aataggcgca ttctgcatgc ccagcatgcg
     3181 cttgacggtg gagacgatct tcggcgcttt ttcttcggtg gattcgacga gcggctgatt
     3241 ggaagccttg gcgagtacct ggcgggcgga ccatttgccg tagccgaggt cggcgtggag
     3301 atcgtctatg tggccgcagc cgtattcgga ggcgacgcgc tgccagtctt catcggcaat
     3361 tttcttgagg ctgatgccgc tgtggcgtgc ttcgcgctca aggagacgac ggccgaccgt
     3421 ggtggcttcc tcatgctcgt gaagattaat ccagtggcgg attttgctgc ggctgcgaga
     3481 ggtctggacg aatttgagcc agtcgcggct ggggccgtga tttttctgcg taataatttc
     3541 gacaacgtcg ccattggcga gtacatggcg cagaggaacg atctggccgt tcactttcgc
     3601 gccgacacac tggtggccga cttcggtgtg aatggcatag gcaaaatcga caggagtcgc
     3661 gccgcgcgag aggacgacga ctcgtccttt aggcgagaag gtgtaaacct cctcgggata
     3721 gagatccact ttgagagtgg agagaaattc gctgggctcc tccatctcct gaacccactc
     3781 gatgagctgg cgcatccaga cgacgcgttg atcatcgcgc tggtcgtcta catccacgcg
     3841 gccgtccgca ccggggcgac cggagggtgg acgcgcatct ttgtacttcc agtgagcggc
     3901 gacgccctgt tcggcgatgc ggtgcatttc ctgtgtgcga atctgaattt cgaacggctg
     3961 cccggagtga agcacggtag tgtgcaagga ctgatagagg ttgggacgag gcatcgaaat
     4021 gtaatcttta aagcgtccgg gaacaggccg ccagatctga tgaacgacgc cgagggccgc
     4081 gtaacagttt tttaccgtgt cggtaacgac gcgcacggcc atcaaatcat aaatctggtc
     4141 aatagagcgc tgttggcgaa gcattttctg gtgaagcgag aaaaggcctt tggtgcgccc
     4201 ttcgacttcg gcgggaatgc cgctttcgac catgcgcgtg cgaatcgcgt cttgcacttc
     4261 gtggaggaat ttttcctgca gcttgtgctt ggtgacgact tttttctgga gttcgagaaa
     4321 cgcggcgggt tcgaggaagc ggaaggcgag gtcttcgagt tcgccgcgca tgacgcccat
     4381 gccgaggcga ttggcaaccg gcgcgtagat ttcgagcgtt tcggtggcga tgcggcgctg
     4441 gcgctcgggg tcgagaaatt cgagcgtgcg catattgtga aggcggtcag cgagtttgac
     4501 gaggatgacg cgaacgtcgc tgaccatggc gagaagcatt ttgcggacgt tctcggcctg
     4561 gcgcgcttcc ggagcgagca ggtcaatgcg gctgattttg gtagcgccgc cgacgactcg
     4621 agcgacatcg gcgccgaaca tttccgagat acggtcgatg ggaatgcgcg tgtcttcgac
     4681 cacatcgtga aggagcgcgg cgcagagcgt ggtgacgtcg agcttcatgt cggcaagaat
     4741 gccggcgacg gcgagcggat gggaaaggta gaggtcgccg gactcgcggc gctgggaacg
     4801 atgctgttcg gcggcaaaat cgtaagcgcg gcggagcaga tcgagatcgt cgcccgggcg
     4861 attgcggcga actttgtcca tcagctcgac aaacttcagg tcgagcacac gctgagcaac
     4921 gggatcgccg gcgggtttgc gttccggact cacggtttcc tcattctacg cccaaacaaa
     4981 aaatctttca cgccgtgcgc tcaaaagaaa accggctggc tcgatttccg ccagccggtc
     5041 caatgtatcg aatagaaaac ggaaagttta gtgcgtgctg ccgggctgcg tggtcatctg
     5101 tttctggtta atttccttca ctttgtcgag cagctgatcc gcctgctttt catcggcatc
     5161 gcgcgctgcc gggtcgctct ccatgtcggc cttttcgcga agaagcaagt tcagataagc
     5221 catggcgttc gcgtaatcgg gcttgatctg cagcgcgtgg ttgagattgt cgatgccttc
     5281 cttcaccgtg tcgccctctt tctgcacaaa atcgtcgcga actttcgcgg gaagcgggtc
     5341 gagcggcctg agcggtttct tggccttctg attgtaatcg gcacgaagct gcgcgttggt
     5401 gcggaaggca atccagtaat caatcacccc gacccagtag tagggtgtcg catcattggg
     5461 attgagcgcg agagcttttt cctgatagtc cttggcctgc tccatcatgt cgcgattgaa
     5521 tgggctgccg gccatgttgt agtacatgga gccaatgccg gtgagagccg tggtgttatt
     5581 gggattcgat tcgaggactt tcttccatac ggcgatagcc tgctcgccat tgcgcttgtt
     5641 ctcttccgaa ggagcgccgg gaatgtactg ctgcgcgtaa gcctgagcga gaaagagctg
     5701 cgcgttggtg agcttggggt cattctgcgt agcgtccttg aaatactcga tggcctgctc
     5761 gaactgcgcg ttgttgtagg cttgcacgcc tttgttcagg tcgtcgcggg ccttcagctt
     5821 ggcgcaacca gccgcgccca atgcaagaag caatactgat gctgacaaaa tcgccgcagg
     5881 cttgcgattc atcctgtctg tctcctcgaa gtttttgcga aacttacaag ccagtgctgc
     5941 tgccggcttt ccattcacgc ataaagcggc gatgaataga atgcgcgcga ctttccgggc
     6001 agctactggc cggcttccat tttggccgta atcaggccga cgtcggtgat gcccactccg
     6061 tgcatgatgt cgatggcatg agcgacgtag gcaaattgcg tgtcgtcatc gcccttgacg
     6121 aaggcgatct tttgcgcgcg gatcttgaag atgttgtcca gttgcggccc aagctccgcc
     6181 cacgaggtcg gcgtctgatt gatgctgact ttgccatcgc tgcttatgac cacgacgatg
     6241 gtcgtgtcgt tctgctgctg ctgttgctgc tttttcggcg gcggctgcgg cacatcggcg
     6301 gccatgccgg tgggcgtggt cggagtgatg gccatgaaga tgatgatcag cacgagaaga
     6361 acatcgatca gcggaacgac gttcggctcg gacatcgagc tgccgtgccc tcctactgag
     6421 atacccattg cagaatcctc ctgccgtcag agtaggtcag cgtagctacc cgccggcggc
     6481 ttccattttg gccgtaatca ttccgacgtc ggtgatgccc acgccgtgca tgatgtcgat
     6541 ggcatgagcg acataagcga aggccgtgtc gtcatcgccc ttgacgaagg cgatcttttg
     6601 cgcgcggatc ttgaagatgt tgtccaattg cggcccaagc tccgcccacg tggtcggtgt
     6661 ctgattgata ctgaccttgc cgtcgctgct cacgacgaca acgatcgtat cattctgagt
     6721 cggcggcttc tgcgttttcg gcggcggctg cggcacatcg gcggccatgc cggtgggcgt
     6781 ggtcggagtg atggccatga agatgatgat cagcacgaga agaacgtcga tcagcggaac
     6841 gacgttcggc tcggacatcg agctgccgtg ccctcctact gagataccca ttgcgaaatc
     6901 ctcctaaact agcttcccgg cgtcaggttc ggcggcccgg gcggctggtt cgattcatcc
     6961 ttgagtgtga gcaggccgac gttttcgatg ccggcgtcgc gaatggcgtc aatcgccttc
     7021 ttgacgtcgc catatttggc gcgctcatcg ctgcggaggt agacggtctt atccaagcgg
     7081 ttggacattt tgtcctgcac tgtggacgtg atctgattca aggcgatttc gttgttgccg
     7141 aggtaaaagc ggctatcgct ggtgacagcg ataaccacgg cgtcatcgcg ttcggcctgt
     7201 ggcatgttct gccagttctt ggccttggcc atgtcaacca actggtggtt gttcaacatg
     7261 ggcgtgatga ccatcatgat gatcagcaag accagcatga tgtcggccat tgggatgacg
     7321 ttgggggccg ccatggtggc gcccaccttg ggtttatacg gcatggctta cttccttgcg
     7381 cctgcccggc gcgtgatgaa gtagttgacc agctccatcg aggagttgtc catctcgacg
     7441 tcgaaggctt cgactttatt ggtgaagtag ttgtaagtcc acacagcggg gacggcgacg
     7501 agcaagccga gcgcggtggt gacgagggct tcagcgatac cgccggcaac cgcggaaagc
     7561 ccgctggcgt gctgcgcggc gatgcctttg aacgcgccga tgatgcccat gaccgttccg
     7621 aaaagcccga cgaacggggc cgtggcgccg atggtggcga gaccggaaag cccgcgcttc
     7681 atctcggcgt ggacaatggc tacggaacgt tcgaggccac gtttggccgc ttcaatgact
     7741 tcttcgtcag ccacttgcgc ggaggccgcc tgaaattccg aaagcccggt agcgacaacc
     7801 ttggcgatgt ggctcttctt attgcgctcg gcgatggaaa tggcttcatc gagtttgttg
     7861 tctttgagcg cgccggcgac ttgctgcacg aagacgcggg actgcttgcg cgccgcgctg
     7921 tacatcagag cgcggtcaat catgatgccg accgaccacg cggacataat gaacaaaata
     7981 atcacgacga cttttgccgg gatgtccatg gtgtgccaca tgctgcgaat gtcccaaccc
     8041 ggcgccccgg cactaccacc ctggtcctgc aaaaacagga acgcagccca cactaagtta
     8101 gaaagcataa cgtccgtccc tcctccgaga gttcaaaagt gattggcagc gaatgcgggc
     8161 cgccaagtta ttctcctaaa gtaaatacta ccgaaatggt tgtgtcgacc ttaactggcg
     8221 tgccgttaag ctggtatggg gcgtacttcc attgccggac agcgtctaat gccgcctgct
     8281 taagaagcgc cggaccggag atgtacgtca aggacgtaat atcaccgtgc tcgtcaatga
     8341 tcgcgtgcaa aacgaccgtg ccctgctgat gagtcatacg ggcgatttgc gggtattcgg
     8401 gcttcacttc ccgaaggatc ttgccttgca tcacgttgcc gcccacgcga atcggtgccg
     8461 ttggcttcgg aggcggagcc ggcaaacctc ccggcgcacc cccgatgatg ccgcccagaa
     8521 cgccgccagc agagccgccc ggcacgcctc cctcaacacc tcctgtgact cccacgtccg
     8581 gcggcggggc ttcctggtga atcatggcaa ttttcttggg gacgacagtc ggggtgacca
     8641 tcttctgctc gaatttcggc tgtggtttta ctttaacaac cgcggctggc ggagggggtg
     8701 gcggaggcgg aggcggagca gccaaaaacg aagtggagag caaaggcgga agagcgcggg
     8761 tgtaaatcag cgggataaga atcaggacga ttaaaatcaa cgcctggatg cccgcagaaa
     8821 cccatacgct ccagaccttg gcggtcgctt tcttgtttgt ggattccaca agttggtcaa
     8881 acattggttt tacctccttg ctgtcgcgag acgcttttcg gcgcgcttat ctgccgaaga
     8941 aggtaccgag atcgtggcgc cgcgctggcc gcgccactta ttccaaacca ccacaatcgg
     9001 ggcggcaatg ccgaaactgg agtacgtacc taccacgacg ccgataacca tcgcaaaggc
     9061 aaaactccgc agcacctgcc cgccgaaaac gaatagcagg aacacggtca agaaagtaag
     9121 tccgctggtg aggatggtgc gggaaagcgt ctggttgatg gagcggttca cgatgacgtt
     9181 caacggctcg cggcgcatca gccgcagatt ttcgcgcacg cggtcgaaaa tcacgatggt
     9241 gtcgttcatc gaatagccaa ccagagtgag tagcgcggca atcaccgtca gcgaaatctc
     9301 aaacttaccg taaaacaggc agaagaagcc gagggtaatc agcacgtcat ggaaaacggc
     9361 gatgaccgcg gccagtccga aaatggtgta gtcaatccag cgccagagga aaaagccgcc
     9421 ggcgttggag aaatgcaaga ggtcgccgcc ggagaagcgg atggcgacgt aaatcaacat
     9481 gcccgcgagc gcgtatagcg tcgcgagaat cgcctgccgg cgcagttgcg cgccaaccgt
     9541 gggtccgacg acctgcgcat tcaaaatgct gaagctgttc aatgaatagc cagccttaag
     9601 agcggcgatc actgcggcgt ttgcgccagc gacgttcgag agctggtcga agttgttgat
     9661 gattccgccg tgctcgcggt cacgcgcatt caccagagat tgggccaatt gcgtgtaacg
     9721 gtcgccggcg gccgtttgca gcatgagcgg atcgcgcttg gtgagatagt tcgcaaggtc
     9781 ggaggtggaa acggcattga aatccggctt cgagctgctg ggcgccccga aattcttttc
     9841 gagcgcgctc aggatggcgg cttttgcggc gttgtcttcg tcttcgccct tggctacctg
     9901 cgaaatgcgg atcatcacgt cgttgcgcga attgggattg gaaatgtcgc tgctgctggt
     9961 gacctcattg ttcttgaggc cgacgttgtc cagcgccgtg cgaatctggc tgatgggagg
    10021 cgcggaagaa aagcgcacga tgacctgcgt gccgccctca aaatcaatgc cgtaatgcaa
    10081 gccgtgatgg taaatggcgg cggcggcgcc aatggcgaga agacacaggg aaagagtgac
    10141 aaagtacttg gtcttcttca tccagtcgaa atttggtgcg tgaaaaaatt ccatcgactc
    10201 ttatccttgc tgaattagac accagccgaa aaagaatgtt cccaaatcgg catggtggcc
    10261 ttggatttgc tctcaaactt cctaaatcac taaaaacaag ctagatactc aactctgctt
    10321 cgcggcccat atgagacaaa tgccagtcga agatgactc
//
LOCUS       DATNDT010000214         3887 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_196350, whole genome shotgun sequence.
ACCESSION   DATNDT010000214 DATNDT010000000
VERSION     DATNDT010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196350"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..252)
                     /gene="rplS"
                     /locus_tag="VN788_06935"
     CDS             complement(<1..252)
                     /gene="rplS"
                     /locus_tag="VN788_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008181294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXU20297.1"
                     /translation="MKPIIAKVQESQLRTDHPSFRPGDTIRVHVRLKEGEGDKERIQP
                     FEGVVMSRRGELLGESFTVRRVSFGIGVERIFPVHSPMIS"
     gene            623..2287
                     /locus_tag="VN788_06940"
     CDS             623..2287
                     /locus_tag="VN788_06940"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXU20298.1"
                     /translation="MLLVLSILIGALTGAAVVALILLTERLGMRLYPVGSPLWRRVLT
                     PIAGSLAMGYVLVRYFPDARGSGVGQTKVALFARDGIITLRAVFGKFFCTAVTLGSGI
                     PLGPEGPSVLIGAGIGSVLARRLKLRPENVKALIPVGAAAAIAAAFNTPLAGVVYSLE
                     GIVGDLNAPVLGSVVLASATSWLVLRLLLGNNPLFVVPQYQLVNSTEFAIYAVLGVVG
                     GLISVALVKLLIGIRSRFLRLPVRTHWFQPVAGGVVVGLMGLWLPQVLGVGYGYVGDA
                     LNGRMAFKLMALLVILKFISVTASYASGNAGGLFAPSLFLGAMVGGTVGSLVHHWWPA
                     HTSVPGAYALVGMGALFAGIMRAPMTSVLMIFETTHDYAVIVPLMIANLVSFWISSAI
                     QPETIDQALSRQEGIHLPTADTLAHVGRRRVAQVMRGATDVLSPSMTVREALARAGQT
                     LLNGWPVTDERGVIGVLSRESLELAVKRFEEEKPLSELLTGLDFPHVHTDQPLHMALE
                     RMGAAKLDVLPVVSRTNIRLLLGVVYLSDILDSFGVAPHEVHDGEF"
     gene            complement(2328..3485)
                     /locus_tag="VN788_06945"
     CDS             complement(2328..3485)
                     /locus_tag="VN788_06945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXU20299.1"
                     /translation="MDKKNIAVIGGSAAGLFAARLLAQRGLAVRVLERTDALEPEART
                     LIVTRRMRTLLGDAAESSIVNEIRRFELFTDGRAATVTLQQPDLIIERRALIQRLAED
                     AAGSGAKIELGRRFHSLRAEGTGVAVEVERCADGSREELHADTVIGSDGATSRVARSA
                     GWAPMPTVPLIQAIVPLPNDMRADTVRVWFMPQDTPYFYWLIPESSKRGALGVIGESG
                     PRTRRCLELFLEKRRLDPIEFQGARIPVYRRWTPVRRNVGRGSVYLVGDAAGQVKVST
                     VGGIVTGFRGALGVAESILNGGSSRELNALRRELDFHLLLRRSMHGFQQSDYSQLVDL
                     LNDSTKQPLGTYTRDEAWKILWRVWLRQPRLILMGLRGLLMRNRLAGTPDA"
BASE COUNT          735 a         1201 c         1074 g          877 t
ORIGIN      
        1 gctgatcatc ggcgagtgta ctggaaagat acgctccacg ccgattccaa aactgacccg
       61 gcgaaccgtg aaactttcgc ccaacagttc acctcgacgg ctcatcacca cgccctcgaa
      121 tggctgaatg cgttctttgt ctccttcacc ctctttcagt cggacatgga cgcgaattgt
      181 atcccctggg cgaaaagaag gatggtccgt gcgaagctgt gactcttgga ccttggcaat
      241 aatcggtttc atgatatttt cccggcagac ccgctcaggt ggggccagcg catcacaaag
      301 atggacgatt atagcctgtt tggccatcgc cgcctagctg tttgccttcc gctcggacaa
      361 tgttctcgtt cagaatgatt ggccgcacca acaatcggca gattgccgcc attcaaatct
      421 atgtcgacca actccgtcca caaaaacggc ctgcacacac atctcctcct tgcgaaaagc
      481 ttttctggca cggccagata aaccgtatga tgagttcgcg tctcggagct tgaattagat
      541 gggcgggagt cacccggaac cgccgttgcc ttcagaaccg cttcgatggt tggccttgcg
      601 ttttggccgc cgcggagaac agatgctgct cgtcttgagc attctgatcg gtgcgttaac
      661 gggcgcggcg gtagttgctc taattctgct caccgagcgc ctcggtatgc gtctttaccc
      721 ggtgggcagt ccgctatggc gcagggttct gacgccgatc gccggatcgc tcgccatggg
      781 ttatgtcctt gtccggtatt ttcccgacgc ccgcggcagc ggtgtggggc aaacaaaagt
      841 agccctcttt gctcgcgatg gcatcatcac cctcagagca gtcttcggaa aatttttctg
      901 caccgctgtt actctcggca gcggaatccc tctgggtcct gaaggtccat ccgttctcat
      961 cggcgcgggt ataggatcgg tccttgcccg tcggctgaaa ctgcgacccg aaaacgtcaa
     1021 ggcgttgata cctgtaggag ccgcagcggc gattgcggcc gcgttcaaca ctccactggc
     1081 tggggtcgtg tactccctcg agggaatcgt tggcgacctg aatgcgcccg tgcttggctc
     1141 cgtggtactt gcctccgcca cctcctggct cgttttgcgc ctgttgctgg gtaacaatcc
     1201 tctgttcgtc gttccccaat atcaattggt caattcgacg gaatttgcga tctacgcggt
     1261 gctcggggtc gtcggcggtt tgatctcggt cgccctggtg aagcttttga ttgggatacg
     1321 ctctcgcttt ttgcgcctac ccgttcggac gcactggttt cagccggtcg cgggaggagt
     1381 cgttgtaggc ctgatgggcc tatggctgcc gcaggtgctc ggcgtcggct acggttacgt
     1441 cggcgatgcg ctaaacggtc ggatggcttt caagctgatg gcgctgctcg tgattctcaa
     1501 attcatttcc gtcacggcaa gctatgcctc cggcaacgca ggaggcctct tcgcgcccag
     1561 cctctttctc ggagccatgg tcggtggcac cgtcggaagc cttgtccacc actggtggcc
     1621 tgctcacact tccgtgcccg gagcctacgc gctcgtcggc atgggcgcgc tattcgccgg
     1681 aatcatgcgc gctcccatga cttccgtcct catgattttc gagaccactc acgattacgc
     1741 tgtgatcgtg cccctcatga ttgccaattt agtgagtttc tggatctctt ccgctataca
     1801 gcccgagacc atcgaccagg cactttcgcg tcaagaaggt attcacttgc ctactgccga
     1861 taccttggcc cacgttggcc ggcgtcgggt agctcaagtg atgcgaggcg ccaccgatgt
     1921 gctctcaccc tcgatgacgg tgcgcgaagc cctcgcacgt gcgggtcaaa ctctattgaa
     1981 tggttggccg gtgacggacg aacgcggcgt tatcggtgtt ctctcccgag agagcctcga
     2041 attggccgtg aaacgcttcg aagaggagaa gccgctctcg gaactcttga ccggcctcga
     2101 tttccctcat gttcacacgg atcagcccct tcatatggca ttagaacgta tgggggctgc
     2161 caagctcgat gtccttccgg tggtgagccg aaccaatatt cgcctgctgc ttggcgtggt
     2221 ctacttgtct gatattctcg attccttcgg tgttgcgccg cacgaagtgc acgacggcga
     2281 attctagcct gtgcacctgc cgccaatttg cccgtttgcc ggcgccgcta agcatcgggt
     2341 gtacccgcca atctgtttcg catcagcagc ccgcggagtc ccatcagaat caatcgcggc
     2401 tgacgcagcc aaactcgcca caggattttc catgcctcgt cgcgcgtgta cgtgccaagc
     2461 ggctgctttg ttgaatcgtt aagcaggtca accagctggc tgtagtccga ttgttgaaac
     2521 ccgtgcatcg agcgccgcag cagcaaatga aaatccaatt cccggcgcaa ggcattcagc
     2581 tcgcggcttg atcctccgtt aagaattgac tcggcgactc ccagcgctcc gcggaatccc
     2641 gtgacgatcc cgcccaccgt tgacacctta acttgtccgg cagcgtctcc cacaagatag
     2701 accgagcctc gccctacgtt gcgccgcacc ggagtccagc gacggtaaac cggtatgcgc
     2761 gcgccttgaa actcaattgg gtcgagcctt cgtttttcga ggaacagttc caggcagcgc
     2821 cgtgttctcg gtccggactc gccaatcacg ccgagcgccc cgcgcttcga cgactctggg
     2881 atcagccagt aaaagtacgg tgtatcttga ggcatgaacc acacgcgcac cgtatctgcg
     2941 cgcatatcgt tcggaagagg cacgatagcc tggattagtg ggaccgtcgg cattggcgcc
     3001 caccccgctg agcgagctac gcgactcgtg gcgccatcgc tcccgataac cgtatcggcg
     3061 tgaagctcct cgcggctgcc gtcggcacac cgctctacct cgacagccac tccggtgcct
     3121 tccgctcgca gcgaatgaaa acgtcgccca agttcaatct tcgctcccga gcctgcggcg
     3181 tcctcggcta agcgttgaat cagggctcgc ctctcgatga tcagatccgg ttgctgcaat
     3241 gtcaccgtcg cggcgcggcc gtccgtgaaa agctcgaaac gacgaatttc attgacgatg
     3301 ctgctctccg cggcatctcc aagaagggtt cgcatccggc gagtcacgat tagcgtccgc
     3361 gcctccggct cgagcgcatc agttcgttcg agcacgcgca ctgctaggcc gcgttgcgcc
     3421 aacagccgcg ccgcgaagag tcctgcagcc gacccgccga tcaccgcaat gtttttctta
     3481 tccattcgtc ggttttacgg ttgttctata caagcccgcc ctgaagccat cggaatccat
     3541 atctcggcat gcgcaccgaa attagaacgc gggcattaag acgccgcgcg aagattcgct
     3601 aaatccccaa atatgagtcc cctggctaaa tgttgggcac cgagtatatc ctacacaccc
     3661 gcccctccaa agcgccgggc gacggcggca taggcactta tgataggatt tatgagctgc
     3721 gccgaggcag gcgtggcgca ggagttaaga catggcgaag ttcacaattg caccgcatat
     3781 gcggcttcac gagtgggtag ccgaggaaaa gggctacttc gctgccgaag gcctggacta
     3841 cgagtttcgg gaagcgctcg cttccgacga agccaagaaa cacaatt
//
LOCUS       DATNDT010000215         9507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_865947, whole genome shotgun sequence.
ACCESSION   DATNDT010000215 DATNDT010000000
VERSION     DATNDT010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_865947"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(413..1624)
                     /locus_tag="VN788_06950"
     CDS             complement(413..1624)
                     /locus_tag="VN788_06950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU20300.1"
                     /translation="MGGQAFQLDPTLRSVESRDFESGLRRKIVGQDEAVQAVVDLYQV
                     FRAGLNSPGRPVGNLLFLGPTGAGKTRVVEATAEVLFGDPRAVIKVDCAEFQHSHEIA
                     KLIGSPPGYLGHRETHPLITQEALAQYHTDKLKISFLLFDEIEKASDALWQLLLGILD
                     KATLTLGDNRRVDLSQTMIFMTSNLGGGEITELMTGGLGFAPEVRAESKPRLDEKVER
                     TASEAAKRKFAPEFMNRIDKVVVFHPLRSQQLEQILEIELGMVQQRVLETAKGRFLFR
                     LTQPAREFLLREGTDLKYGARHLKRAIERHLVYPLASLLATNQVTLGDVLSIDWSAGA
                     SVLTFLKEAEGALVPVISPEPAPLAGAAAAISDGRELSFPTPVTAAAEVPLPLPNHRS
                     APTEKAKNEKG"
     gene            complement(1637..2071)
                     /locus_tag="VN788_06955"
     CDS             complement(1637..2071)
                     /locus_tag="VN788_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20301.1"
                     /translation="MAFVRKKGKRYFLVHNVRRRGKVQQLHLARLGERPRITDEVVRK
                     VARNHPFLDLDWGHLREQVSSRVELFDVKSPYVRNLMHSLRSLNLDLADLSPPLLVLA
                     DRANSSRELVTQLRLLRSTVDIKLDQFERSEPVSRDGGRRYR"
     gene            2334..2678
                     /locus_tag="VN788_06960"
     CDS             2334..2678
                     /locus_tag="VN788_06960"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HXU20302.1"
                     /translation="MSWEPVYRLVKRVPRGRVTTYGSLAKALRLRGGARAVGYAMAAC
                     PSGRGIPWHRVVGAGGRLMIPEPHGSLQRRLLEAEGVEIGERRHVDMPRYIWLPAKKR
                     SSGRKRRPAKPR"
     gene            2737..3657
                     /locus_tag="VN788_06965"
     CDS             2737..3657
                     /locus_tag="VN788_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU20303.1"
                     /translation="MGDPIIQVEDLYKSYGAVEAVRGISFAVERGEVFGLLGPNGAGK
                     TSTVEILEGLRTPDRGVVRVCGLDPSESGHAFKEKIGAVLQSTSLPDKLRVDEAIELF
                     AKFYDRRANTNELLERFQLTEKRSAFYSHLSGGQKQRLALAMALVNDPEVVFLDEPTA
                     GLDPQVRREIYDIIEELKRGRKTVLLTTHYIEEAERLCDRVAIVDHGRVMALGTPREL
                     KHKSAGMTRIEVRLARPITNGLLGHLEGVADCREFQGTYVLHSAKPPQTIVALVKQLE
                     VESNELQSLEMFSPSLEDVFIELTGRRLRE"
     gene            3700..4596
                     /locus_tag="VN788_06970"
     CDS             3700..4596
                     /locus_tag="VN788_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20304.1"
                     /translation="MSQTWTLTTMRIRLAMRNRAFFFFSIVMPLIFLLGAVLFFGRGK
                     TEWMGYILGAILTLTVMGSFWGLSVQLVTFREQGILRRFRLAPLGAGSMLASSILSNY
                     FMALPIVVLEILVCRFAFHMQSWGNLWAIFILVTIGSATFSALGLIVASVTNTMQETQ
                     MINNLIWMGFLFLSGATIPLAAFPGWLQRVALFIPAEYLATGLESAGARMATGYQVLE
                     DSAALALGLVVAFEVSRQLFRWEPEARVPRKAKLWVLAALIPFLLFGTWESVTGRQLH
                     HINQNFQELSGRLAPSQTTEPR"
     gene            4732..5694
                     /locus_tag="VN788_06975"
     CDS             4732..5694
                     /locus_tag="VN788_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004513546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HXU20305.1"
                     /translation="MKAIRVHEFGGPEVLRIEEVPDLKPGSGQLVIRIRAVGVNPADT
                     YMRTGTYTRKPALPYTPGTDAGGTVESVGTGVARFKAGDRVYLAGSLTGTYAEQALCE
                     QHTVFSLPANASFAQGAAMHVPYGTAYRALFQRAQARGGEIVLIHGASGGVGIAGVQL
                     ARAAGLRVIGTVGSDRGKQLVSTEGAHHVLDHKSASHFDEAFSLTEGRGCDVIIEMLS
                     NVNLGRDLTILAPRGRVVVVGSRGTVEIDARNTMTRDAAILGMSLWNATPDDVTSIHS
                     ALVAGLENKTLRPVIGQEIPLSEAPRAHRAVMEAGAYGKIVLVP"
     gene            5731..6162
                     /locus_tag="VN788_06980"
     CDS             5731..6162
                     /locus_tag="VN788_06980"
                     /EC_number="1.11.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013246662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0006972 - hyperosmotic response [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0033194 - response to hydroperoxide
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HXU20306.1"
                     /translation="MQRSAAAVWNGALKDGKGAISTASGVLSNTPYSFTTRFENAKGT
                     NPEELIAAAHAGCFTMALSAQLGTINFTAETLRTIATLSLEKLDAGWTISRVHLEVSG
                     KVPGISPSAFQAAADSAKANCPVSRLLKADITMEAVLEQTP"
     gene            6159..7289
                     /locus_tag="VN788_06985"
     CDS             6159..7289
                     /locus_tag="VN788_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015719581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HXU20307.1"
                     /translation="MTLFPAVIGFLIVAVVLWEAFETIVLPRRVARRWRITVLFYRVT
                     WFPWRSLAHAIKRTRLRETLLSFYGPLSLLCLFIFWAGALIVGFGLLYRSAAVANAIS
                     QSISTCVYLSGTTFFTLGLGDVVPHVGVERLLAVTESGLGFGFLALVLSYLPVIYQAF
                     SRREVNIVLLDARAGSPPTACELLRRHTGPGGVEALDQVLHDWERSAADILESHVSYP
                     AVSYFRSQHNNESWLAAFTAILDATALVLTGVENIASRQARLTFAICRHTVVDLTQVF
                     NLRPHRGDADRLPAGELQRLRTALAQAGVQLRDDEAAVAKLVKLREMYEPYLRALSDF
                     LHMELPPWILAKEITDNWRTSAWGRITGFSSSGPREPDDHAD"
     gene            7400..8095
                     /locus_tag="VN788_06990"
     CDS             7400..8095
                     /locus_tag="VN788_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20308.1"
                     /translation="MRSSVLPAARATLAAFLAMGMVNLPLMAAASKPLGMVVAADHAY
                     LDHANAASGASVYSDDALITDEGGSLRLSVGASQIYLLSLSSASLEPRNDKVQARIDR
                     GTMGFSTARPSQLQIQTPVGLVHGADANPIFGQVSLLSPSKLRISSYEGTLVVDDVSG
                     ERKIIAQGETYEATLVSEASGGSDNNPPKKGATGAVVNWSHVLKVAIPIVAAGFVACW
                     LYPESPSKMGCWN"
     gene            8153..8584
                     /locus_tag="VN788_06995"
     CDS             8153..8584
                     /locus_tag="VN788_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20309.1"
                     /translation="MSETNTKKPPQSESSEEHLGVVHENLEGWIPPLATEAEIRAALE
                     KAFDYRGDLTITLKNGRNIEGYAFDRRMNGPSLSECFVRVMPKDQPEKLSIAYSDIAA
                     LAFTGRDTAAGKSFAAWVKKYNEKKAAGEANVGIEPDSLDE"
     gene            complement(8651..9157)
                     /locus_tag="VN788_07000"
     CDS             complement(8651..9157)
                     /locus_tag="VN788_07000"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXU20310.1"
                     /translation="MDSANITYASQTKAFAKRVRAFAAVVFAGVMVAAGVGAIRAAKA
                     ADTAKTTMDGVYTAEQAQRGKAQYSQSCATCHMDDLSGSGQALPLAGDAFMGTWEGQS
                     VADLMGLIHDTMPQDKPGTLTPDQTLDTIAYLLQYNQFPAGKEELKNDPNELKTIIIT
                     KKAAPPAK"
     gene            9359..>9507
                     /locus_tag="VN788_07005"
     CDS             9359..>9507
                     /locus_tag="VN788_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXU20311.1"
                     /translation="MIKRRLGKSNLEIGPLVFGGNIFGWTADEPTSFKLLDAFVASGL
                     NMIDTA"
BASE COUNT         1949 a         2672 c         2798 g         2088 t
ORIGIN      
        1 agttcaggta ggtcccgcag gcgaccgcgt gaaagtcgat gtgccggtaa cgcaggatca
       61 actgctcgct ttaattcgca gccacgcatt cgccacgccg atgtaaaaga gttgggctct
      121 cccatactca agcgctctga aagagcctat cctcgaaaaa gctccgcatc catctaagag
      181 gtagttttac cggggggggg aagatcgttt tccgccaggt gaccttagtg atgatcatcc
      241 gtgtcagcta aacgcacatc gcacgcttcg cggcagtcgg gacaatagta cagtccgtcc
      301 acgcataggt ggacgtgctc ttgcgactgg caaaaacagc aaaacttatc gcaggagcat
      361 ttgctctcag acttgtcgca ttgagaacag agggtgacca ccagcggagg gtctatcctt
      421 tctcgttttt ggccttttct gtcggcgcac tgcggtggtt ggggagaggc agcggaacct
      481 cggcagcggc cgtcaccggg gtcgggaaag acaactccct tccatcggag attgcagcag
      541 cggcgcctgc gagaggtgcc ggctcgggcg aaatcacggg aacgagagcc ccctcggctt
      601 ccttcaagaa cgttaagacc gaggcgcctg cgctccagtc aattgagagc acgtcaccca
      661 gagtaacttg gttggttgcg agcaagctgg ccagcgggta aaccagatgg cgctcgatag
      721 cgcgcttcaa gtggcgcgcg ccatatttca gatccgtccc ttcgcgcaga aggaattccc
      781 tggccggttg agtaaggcgg aagaggaagc gtcctttcgc ggtctcgaga acgcgctgtt
      841 gtaccattcc cagttcgatc tcgagaatct gctcgagttg ctgagaccgc aagggatgga
      901 aaacgacgac cttatcgatc cggttcatga actcgggtgc gaactttctc ttcgcagcct
      961 ccgaggcggt tcgttcgacc ttctcgtcaa gtctaggctt cgattcggct cggacttcgg
     1021 gcgcaaaacc gaggccgcca gtcattagtt ccgttatttc tccgcctccc agattcgagg
     1081 tcatgaagat catggtctgc gacaaatcga cgcgacgatt gtcgcccaga gtcagcgttg
     1141 ccttgtcgag aatgcccagc agcaattgcc acaaagcgtc gctggccttt tcgatttcgt
     1201 caaacagcaa gaagctaatc ttgagtttgt cggtgtgata ctgggcgagg gcctcctgag
     1261 tgatgagcgg atgcgtttcg cggtgcccca aatatcccgg aggagagcca atgagcttgg
     1321 caatttcgtg cgaatgctga aattcggcgc agtcaacctt gatcacggcg cgggggtcgc
     1381 cgaacagcac ctcggctgtg gcttcgacga cgcgggtctt gccagcgcca gtgggtccga
     1441 gaaacagaag gttccccaca ggccgccccg gtgaatttag accggcgcgg aaaacctggt
     1501 ataggtccac aacggcctgc accgcctcat cctgtccgac aatttttcga cgtagcccgc
     1561 tttcgaaatc ccgcgactct acgctacgca gggtcgggtc gagttgaaac gcttgtccac
     1621 ccatggtctt gcccccctac cggtaccttc gcccgccatc gcgactgacc ggttcggacc
     1681 gctcgaattg atccagcttg atatcgaccg tcgaacgtag cagtcgcaat tgcgtgacca
     1741 gttcccggct tgaattggcg cggtccgcca gcaccagcag tggcggcgac agatcggcca
     1801 ggtccaggtt caggctgcgc agcgaatgca ttagattgcg cacgtacggc gatttcacgt
     1861 cgaacaactc tacccgactg ctcacctgct cgcgcaggtg cccccaatcg agatcaagga
     1921 agggatgatt tcgggcaact ttccgaacca cctcatccgt gatacgcggc ctttcaccca
     1981 accgcgccag atgcaactgc tgcacttttc cgcgccggcg aacgttgtgc accaggaagt
     2041 acctttttcc ctttttccga acgaacgcca cagcgttctc cttccggccc gaacacccta
     2101 aaacagcccg tagtgtaagg accatccact acacagtcaa tggtacgatg ctgtggactg
     2161 gggaaggttg cttcgaattg gttattactc ttgcggaacg gttgcggggg cgcaaacaag
     2221 aaggttagac gagctcacgt tgagttctaa aatgggttcg ataaacaaaa ctttccctta
     2281 ttgatttaga tgcggaattt gcaaggtggg ttttcggacg ggagctatag aaaatgtctt
     2341 gggagcccgt ttatcggctg gtgaaacgag taccgcgcgg acgcgtgacc acgtacggga
     2401 gtcttgcaaa ggcccttcgg ctacggggcg gagcgcgcgc cgttggctac gcgatggcgg
     2461 cctgtccgag cggtcgaggc atcccctggc atcgcgtggt tggcgcgggg ggacggctga
     2521 tgattcccga gccgcacggg agtcttcagc ggcggctgct ggaggctgag ggcgtcgaga
     2581 tcggtgagcg caggcacgtg gatatgccgc gttatatttg gctgcccgcg aaaaagcgct
     2641 cctccgggcg taaacgccgg cccgcgaagc cgcgctgatt cgcgctgcca ttgcgactgt
     2701 gataaaagta ttgtttcgat acgcaggact tattctttgg gcgacccaat catacaggtc
     2761 gaggacctct acaaaagcta tggcgccgtg gaagcggtgc gcgggatcag ctttgcagtt
     2821 gagcgcggcg aagttttcgg cttgctgggc ccaaatggag cgggcaagac atccacagtg
     2881 gaaattctgg agggcctgcg cacgccggat cgcggcgtag tgcgcgtgtg cggactcgat
     2941 cctagcgaat ccggccatgc tttcaaagaa aagatcggag cggtgctgca atctacgtcc
     3001 ttaccggaca aactgcgcgt ggacgaagcc attgagctct tcgcgaaatt ctatgaccgc
     3061 cgcgcgaaca ccaacgaatt gctggagaga tttcagttaa cggaaaaacg cagcgcgttc
     3121 tacagccatc tttccggcgg gcaaaaacag cggctggcgc tggccatggc gctggtgaat
     3181 gatccggaag tggtctttct ggatgagccg actgcgggcc tcgatccgca ggtgcgccgc
     3241 gaaatctacg acatcatcga ggaactcaag cgcggccgga agacggtgct gctgaccacg
     3301 cactatatcg aggaagccga gcgattgtgc gaccgggtgg ccatcgtcga ccatggccgg
     3361 gtgatggcgc tgggcacgcc gcgagaattg aagcacaagt ccgccggcat gacgcgaatc
     3421 gaagtacgtc tggcacggcc gattacgaac ggcttgctcg gccacctcga aggtgtggcg
     3481 gactgccgcg aattccaagg cacgtatgtg ctgcactccg caaagccgcc gcagacgatt
     3541 gtggcattgg tgaaacaatt ggaagtggaa agcaatgagc tgcaaagttt ggaaatgttt
     3601 tcaccgtcgc tggaggatgt gtttatcgaa ctaaccgggc gccggctgcg cgaatgaacg
     3661 cctggttaaa accgagctat tgaaagtgga gcaaatactt tgagtcaaac gtggaccctc
     3721 acgacgatgc gcatccggct ggcgatgcgc aatcgcgcct tctttttttt cagtattgtg
     3781 atgccgctta tatttttgct gggcgcggtc ctcttcttcg gcaggggcaa gacggaatgg
     3841 atggggtata tcctcggcgc catcctgact cttaccgtga tgggaagctt ctggggactc
     3901 agcgtgcaat tggtaacttt tcgcgaacag ggcatcctgc ggcgatttcg gctcgcaccg
     3961 ctgggtgcag gatcgatgct ggcgtctagc atcctttcca actatttcat ggccctgccg
     4021 atagtggtat tggaaatttt agtctgccgc ttcgcgttcc acatgcaatc gtggggaaac
     4081 ctttgggcca ttttcatcct cgtgactatt gggtcggcga cattttcggc gcttgggctc
     4141 atcgtggcta gtgtaaccaa caccatgcag gaaactcaga tgatcaataa cttgatctgg
     4201 atgggtttcc tctttctttc aggagcgacg ataccgctgg ccgcctttcc gggctggcta
     4261 cagcgtgtgg cactttttat tcccgctgag tatctggcta ccggtctgga gtctgcgggc
     4321 gcgcgaatgg cgacaggcta tcaagtgctc gaggatagcg cggctctcgc gctcggactt
     4381 gtggttgcct tcgaagtctc gcggcagctt ttccgctggg aaccagaggc cagggttcca
     4441 cgcaaagcaa aactgtgggt tctggcggcc ctgattccat ttttgctgtt tgggacttgg
     4501 gagagcgtta ctggaagaca gttgcaccac atcaatcaaa attttcaaga gctgagcggg
     4561 cggctcgcac cttcgcaaac gactgagccg cgctgagtcc gatgcgacgt gaccggctgc
     4621 tgtttttcgt tgaatctact tccggtgcat ttacatcgaa agtgagatca atggcttcga
     4681 agaatctcac cgaacccggc ggcagtatca atttccgata gagagatacc catgaaagcg
     4741 attcgcgtgc acgaattcgg cggacctgag gtattgcgca tcgaggaagt ccccgatctg
     4801 aagcccggca gcgggcaact tgttattcgc attcgcgcgg ttggcgtgaa tcccgctgat
     4861 acgtacatgc gcacgggaac gtacacgcgc aaaccagcgc ttccgtacac tcccgggacg
     4921 gacgccggcg gtacagttga atcagttgga actggagtcg cgcggttcaa ggccggcgat
     4981 cgagtttacc ttgcgggcag cttgacaggc acctacgcgg agcaagcatt gtgcgagcaa
     5041 catacggtgt tctcgctgcc agcaaatgcg tcgttcgcac aaggtgcggc gatgcacgtt
     5101 ccgtacggta ccgcctaccg cgcgcttttt caacgagcgc aggcgcgcgg cggagaaatc
     5161 gtgctgattc atggcgcaag cggcggcgtg ggcattgccg gcgttcagct tgcgcgtgct
     5221 gcaggtctcc gagtgatcgg gacagtggga tcggatcggg gcaagcaact cgtttcgacg
     5281 gaaggggcgc atcacgtcct cgatcacaaa tcggcgagcc attttgacga ggcgttttca
     5341 ctcaccgaag gacgcggctg cgatgtgatc atcgaaatgc tctcgaatgt aaaccttggg
     5401 cgcgacctca cgattctggc acctcgcggc cgcgtggtgg tggtcggcag ccgcggcacc
     5461 gtcgaaatcg atgcgcgaaa tacgatgacg cgcgacgccg cgattctcgg aatgagcctc
     5521 tggaatgcca ctcccgacga tgtcacgagc attcattccg cccttgttgc gggactcgaa
     5581 aataaaacgt tgcgtccggt gatcggtcag gagatcccgc tctccgaggc tccacgcgcc
     5641 cacagagctg tgatggaagc gggcgcgtac ggcaaaattg tactggttcc ctaacctttc
     5701 gctacacttc gcgcacaagg agagcacacc atgcaacggt cagccgcggc tgtttggaac
     5761 ggcgcactca aagatggaaa gggggcaatt tccaccgcga gcggcgtcct ttcgaacacg
     5821 ccgtattcgt tcacgacacg attcgaaaat gccaagggca cgaatcccga ggaattgatc
     5881 gccgcggcac acgccgggtg ctttacgatg gcgctctccg cgcagctcgg aacgatcaac
     5941 ttcacggcag agacccttcg aacgattgcc accctatcgc tcgaaaaact cgatgcgggc
     6001 tggacgattt cgcgcgttca cctcgaagta tctggcaagg tcccgggcat ctcgccatcg
     6061 gcgtttcagg ccgccgcgga tagcgccaag gcgaattgcc ccgtctcgcg gctgctgaag
     6121 gcggatatca cgatggaagc agtgttggaa caaactccgt gacgctcttt ccggcggtca
     6181 tcggcttcct gatcgtggcc gttgttttgt gggaggcgtt tgagacgatc gtcctgccga
     6241 ggagagtcgc tcgccgctgg cggatcacgg tgcttttcta tcgcgttacg tggttcccct
     6301 ggaggtcact ggcacatgcg atcaagagga ccaggctccg cgaaacattg ttgagtttct
     6361 acggtcctct ttcactactt tgccttttca ttttctgggc cggggctctg atcgttggct
     6421 tcggtcttct ttaccgcagt gcggcggtcg cgaatgccat ttctcaatcc atttccacat
     6481 gcgtctacct cagcggaacg acattcttta cgctggggct tggcgacgtg gtgccccacg
     6541 ttggcgtcga gcgccttctc gcggtaacgg aaagcggact cggctttggc ttcctggcct
     6601 tggtgctcag ctatttgcca gtcatctatc aggcattttc acgccgcgag gtaaacatcg
     6661 tcctcctcga tgcccgcgcc ggttctccgc caacggcatg cgaacttcta cggcgtcaca
     6721 ctggaccggg tggcgttgag gccctcgacc aggtgctgca tgactgggag cgatccgctg
     6781 ccgacattct ggaaagtcat gtgtcgtatc ccgcggtaag ctacttccgc tcgcagcaca
     6841 acaacgagtc ctggctggcg gcgttcacgg cgattctgga tgccaccgcg ctcgtgctca
     6901 ccggcgtgga aaacatcgcg tcacggcagg ctcgcctgac gtttgctatc tgcaggcaca
     6961 ccgtcgtcga tctgacgcag gtcttcaact tacgtccgca tcggggagac gccgaccgct
     7021 tgccggctgg cgaacttcag cggttgcgaa cggccctggc acaggctggc gttcagctgc
     7081 gagatgacga ggccgccgta gccaagctcg tgaagctccg cgaaatgtat gaaccgtacc
     7141 tccgcgccct ttccgacttt cttcatatgg aacttccccc gtggattctc gcgaaagaaa
     7201 tcaccgataa ctggcgcacc agcgcctggg gccggattac gggcttttcc tcatccggtc
     7261 ccagggaacc ggacgatcat gcggactgaa accacgtcaa ttgccggtcc cagccgggca
     7321 ccatacctcg cgcggtaaag atattgactc cttccgaagt tgcgcgctaa attgaaattg
     7381 aagttctgag gagggctttt tgcgctcgtc cgtcctgccg gctgcccgcg ccactctggc
     7441 tgcatttctg gccatgggca tggtaaacct gccgttaatg gctgcagctt ccaagcctct
     7501 gggcatggtt gtcgccgccg accacgccta tttggatcat gccaatgcgg caagtggtgc
     7561 gagcgtctat tccgatgacg ccctcattac cgacgagggg ggatccctac gactcagtgt
     7621 cggggccagc cagatttacc ttctttcttt gagttccgcg agcctcgaac cgcggaacga
     7681 taaggttcaa gccaggatcg atcgaggcac gatgggcttc tccaccgccc ggccgagcca
     7741 actccaaatt cagacaccgg tgggtctagt gcacggagca gatgcgaatc ccattttcgg
     7801 tcaagtgagc ctgctgagcc catcaaagct gagaatcagc tcctacgagg ggactctggt
     7861 tgtggacgat gtaagcggcg agcggaagat cattgcacaa ggtgaaacct acgaagcgac
     7921 cctggtgtcc gaagcttcgg gcggtagtga caacaacccg cccaaaaagg gcgccaccgg
     7981 agcggttgtt aactggtcgc atgtcctcaa ggttgcaatt cccattgtgg cagcgggatt
     8041 tgttgcgtgc tggctctatc cggaaagccc atcaaaaatg ggctgctgga attagttgtc
     8101 ggcgcgaaat cgccttctca cgaattttcg gcctgctgaa attttcaacc ccatgtcaga
     8161 aactaatact aagaaaccgc cacaaagcga atcgagcgag gagcatctgg gtgtagtcca
     8221 cgaaaatctc gagggctgga ttccgcctct cgccaccgag gccgagattc gggctgcttt
     8281 ggagaaggca ttcgactatc gcggcgattt gacaattact ctgaagaacg gacggaacat
     8341 cgaggggtat gcctttgatc gcagaatgaa tggtccatcc ctctccgagt gtttcgtccg
     8401 ggttatgccg aaggaccagc ccgaaaaact ttccattgcc tatagtgata ttgccgcgct
     8461 ggcgtttacg ggccgtgaca cggctgccgg aaagagcttc gccgcgtggg tgaagaagta
     8521 caacgagaag aaagctgccg gggaagcgaa cgtcgggatt gagcccgact ctctcgacga
     8581 atagcctgtg gtcatcctga tggtatagtc ggcaacgacc gctagaatgc ccgcgccaaa
     8641 cgccctatga tcatttcgcg ggcggagcgg cctttttggt gatgatgatg gtcttcagct
     8701 cattgggatc gtttttcaat tcttccttgc ccgccggaaa ctggttgtac tgaagcaagt
     8761 acgcgatggt gtctagggtt tgatcaggcg ttaacgtgcc gggtttgtcc tgcggcattg
     8821 tatcgtggat caggcccatc aggtcggcga cgctttgccc ttcccacgtt cccatgaacg
     8881 catcaccggc tagcggtagg gcctggccgc tgccgctcag gtcatccatg tggcaggtgg
     8941 cgcagctctg gctgtattgc gctttccctc gctgggcttg ttcagcagtg taaacgccgt
     9001 ccatcgtggt cttagcggtg tccgcggcct tcgcggcgcg gatcgctcca actccggcag
     9061 ctaccattac gcctgcgaaa accactgctg cgaaggcgcg cacccgcttg gcgaaagctt
     9121 ttgtctggga agcataggta atattggccg agtccatatg tatggttggt cccctcgttt
     9181 tattagatgc gaatattctg tccaaggata ataatagcaa atcttctatg taattcggcg
     9241 cgtcatctgt tcggtccata tgttgccatt gcggctaggc catccgctcg actaccatcg
     9301 ccattgcgac aaccgtccga gcaacagtta aatttgcccg cggcatagga gcatttacgt
     9361 gatcaagcgc aggctcggaa agtcaaactt ggaaattgga ccgttggttt tcggcggcaa
     9421 catatttggc tggaccgcgg atgaacccac ttcgttcaag ctgctcgacg cattcgtagc
     9481 ctcggggctg aacatgatcg ataccgc
//
LOCUS       DATNDT010000216         4134 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2596041, whole genome shotgun sequence.
ACCESSION   DATNDT010000216 DATNDT010000000
VERSION     DATNDT010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4134
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2596041"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="VN788_07010"
     CDS             complement(<1..272)
                     /locus_tag="VN788_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20312.1"
                     /translation="MTRKVRFKAVHYGATAVALSLFVFASGCKKKVPPPPPPPPPAAT
                     TPAAPSAKPTIDSFTVEPSTIQKGQSAVLRWSVTDATDISIDHGVGP"
     gene            complement(321..1676)
                     /locus_tag="VN788_07015"
     CDS             complement(321..1676)
                     /locus_tag="VN788_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translocation protein TolB"
                     /protein_id="HXU20313.1"
                     /translation="MKKTIFACLVSGLLALPAAAQVNEIKIVGNGQIAMAVPDFRGAG
                     GAQSYMTAFNSALFEALQNSGQFRMVPKTMMPLQVPQQPMDLKPAPTPTTKGSGNYLS
                     DWSSPPANANYLAFGYTAEKDEQFVLYGWLYDVTRQDMTSAQIIGKVYFGPLSDDGAK
                     KVARDFAADILAIFGGKGLVGTKIYFVSDRTGHKEIWSMDYDGSNQKEFTHYGSITTF
                     PIISPDGTKLAFLTYARGIPDIFIHSLETGRKLPFYNPRASMNAPSDFMPDSQHLLLY
                     SSAGGGYSQIYLADLDGGNLKRISNSRSSDVEAKVNPKTGADLVFVSGRSGLPQIYAM
                     SIDGINVRRLTDGVGEAVNPAWSPDGKHIAFAWTRGYEPGNYNIFIMDVATREFTQLT
                     HGAGRNENPVWAPDGVHLVYSSKRGRATEIFTMRADGTGQQQLTTVGDNMKPVWSKAS
                     N"
     gene            complement(1673..2455)
                     /locus_tag="VN788_07020"
     CDS             complement(1673..2455)
                     /locus_tag="VN788_07020"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXU20314.1"
                     /translation="MAHVEVLDEQDSWRSPLIGSIAIHAGIVVIAIAYSLYAHGPTMG
                     SSKDLGGSAVAINPVDTIPLPPRAGIKNPLANDTNSIAPLPPPKPQPKPVAPPPPAPD
                     AVRLKHVEKKEKPKPPHEEPVQQKFRREPPREDQVYTQTPPRLISPMISSPGSGGIGV
                     GSHSTIGTEFGWYVDIVKQRVAQHWRTGDLGLATAPIVIVQFDINRDGSINTPSVMQR
                     SGNYALDTSTVRAILDASPFPQLPPGFSRSSATFEIWFQLQK"
     gene            complement(2459..2917)
                     /locus_tag="VN788_07025"
     CDS             complement(2459..2917)
                     /locus_tag="VN788_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HXU20315.1"
                     /translation="MAFSVGGSSSPNGSVFGNKRFRGNTQLAEINIIPLVDVVLVLLI
                     IFMLTAHVMEFGMEVEVPKVDTTQDTAKELPVITISRDGDSFLNEKRVNIHDIGRAVK
                     SRFQNANSVYLRADRQANWDQVAQVIAACSQAKLAVNVVTQPEDHAARRR"
     gene            complement(2921..3607)
                     /locus_tag="VN788_07030"
     CDS             complement(2921..3607)
                     /locus_tag="VN788_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HXU20316.1"
                     /translation="MTPTLFGAIFLQLSLWELVKTAGPIAKVVMVILVVLSILSWAVI
                     LAKWGLFRRAKIANRQFLRAFRKTPGLEAVAVASEQFRASPLVTVFDFGYSEFERQRK
                     TKGDVSNKLALERSLQLGISEEIERLERSMGILATTASVSPFIGLFGTVMGIIDAFSS
                     LGQAGSASLRAVAPGISEALITTALGLAAAIPAAIAYNLYGGTIKEFGTRMEDFGLEF
                     MNMTERSFEE"
     gene            3862..>4134
                     /locus_tag="VN788_07035"
     CDS             3862..>4134
                     /locus_tag="VN788_07035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20317.1"
                     /translation="MNELWSARHYKKTKRTQRGLLAGGAAALISPRAPVLGRPRQQIE
                     VTSTGGYFPFAFMHLMALHESVNCLPSYSGHGGMNELRSDRHYKKTK"
BASE COUNT          857 a         1251 c         1149 g          877 t
ORIGIN      
        1 gggcccacgc catggtcgat tgaaatatcg gtcgcatccg ttaccgacca gcgcaatacc
       61 gccgactggc ctttctgaat ggtggacggc tccactgtga agctatcgat tgtcggcttc
      121 gccgaaggcg ccgccggcgt agtcgcggcc gggggcgggg gcggcggagg cggcggaacc
      181 ttcttcttac atcccgacgc aaacacaaac agcgaaagcg ctactgccgt agcgccgtaa
      241 tgcacagctt taaatcgcac tttgcgggtc atttactcga tctctcctct ttgtccttcc
      301 tcttgggcaa tgaagtaatt tcagttgcta gccttactcc aaactggttt catattgtcg
      361 ccgaccgtgg tgagctgttg ctgccccgtg ccgtccgcgc gcatcgtgaa aatttcggtc
      421 gcgcgccccc gtttcgacga atacaccaag tgaacgccgt ccggcgccca cacaggattt
      481 tcatttctcc ccgcgccgtg ggtcaactgt gtaaactccc gcgtcgcgac gtccatgatg
      541 aagatattgt agtttccggg ctcgtagccg cgcgtccagg caaacgcgat gtgcttgcca
      601 tccgggctcc aggccggatt gaccgcctct cctactccgt cggtcaaacg cctcacgtta
      661 attccatcga tgctcatcgc ataaatctgg ggcaggccgg accggcccga tacgaacacg
      721 aggtccgctc ctgttttcgg attcaccttc gcctcgacat cgcttgagcg cgaattggaa
      781 atccgcttga gattgccgcc atccagatcc gccaggtaga tctgcgaata gccccctcca
      841 gccgacgagt agagcagcag gtgctgactg tccggcatga aatcggacgg cgcgttcata
      901 gaagccctcg gattataaaa gggcaacttc ctccccgttt ctaacgagtg tataaatata
      961 tcaggaattc ccctcgcgta ggtaaggaag gcaagttttg tgccgtccgg cgaaatgatt
     1021 ggaaacgtcg tgatcgaccc gtaatgcgta aactccttct gattcgatcc gtcgtagtcc
     1081 atggaccaga tctctttatg gccggtgcga tcggacacaa agtagatctt ggtcccaact
     1141 agtccttttc cgccgaagat agccaggata tcggccgcga aatcgcgcgc cacctttttc
     1201 gccccatcgt cgctcagcgg cccaaagtag acttttccga tgatttgggc gctagtcatg
     1261 tcctgccgcg tcacgtcgta taaccagccg tagaggacga actgctcgtc tttctcggct
     1321 gtgtaaccaa aggcgagata attggcgttc gcgggcgggc tggaccagtc gctcagataa
     1381 ttgccgctgc ctttcgtggt cggcgttggc gcgggcttca gatccatcgg ctgctgcgga
     1441 acctgcaagg gcatcatcgt tttcggcacc atgcggaatt gcccggaatt ctgcagcgcc
     1501 tcaaacaggg ccgaattgaa cgcggtcata taactctgcg ccccaccggc gccccggaag
     1561 tcgggcacgg ccatcgcgat ctggccgttg ccgacaattt tgatctcatt tacctgagcg
     1621 gctgccggca gcgcgagcaa cccggacaca agacaagcga atatcgtttt tttcattttt
     1681 gtaactggaa ccagatttca aatgtcgccg agctgcggct gaagcccggc gggagttggg
     1741 gaaacggcga cgcatccagg atggcgcgca ccgtggaagt atccagcgcg taattgccgc
     1801 tgcgctgcat aacgctagga gtgttaatag aaccgtcgcg attgatatcg aactgcacga
     1861 tcacgatggg cgcggtcgcg agtccaagat cgcccgttcg ccagtgctgc gccacgcgtt
     1921 gcttgacgat atccacatac cagccgaatt ccgtgccgat ggtgctgtgg ctgccgacgc
     1981 cgatcccgcc cgagccgggc gacgaaatca tcggagagat caggcgcggc ggcgtttggg
     2041 tataaacctg atcctcgcga gggggctcgc gcctgaactt ctgctgcacc ggctcttcat
     2101 gcggcggctt gggcttttcc ttcttctcga cgtgcttcag ccggacggca tccggcgcgg
     2161 gcgggggagg cgccaccggt ttcggctgcg gcttgggcgg cggcagcggc gcgatcgaat
     2221 tcgtgtcgtt tgccagtgga tttttaatac ccgctcgcgg aggaagcgga atcgtatcca
     2281 ccggattgat ggccaccgcg cttccgccaa gatccttcga gctgcccatc gtcggcccat
     2341 gcgcgtacag actataggcg atcgcgatca cgacaatccc cgcgtgaatc gcgatcgagc
     2401 cgatgagcgg acttcgccac gaatcctgct catcaagtac ctcgacatgc gccatacgct
     2461 atcgccgccg ggccgcgtga tcctcgggct gcgtgaccac gttcaccgcc agttttgcct
     2521 ggctgcacgc cgcgatcact tgcgccacct gatcccagtt cgcctgccgg tccgcccgca
     2581 aatacaccga gttcgcgttt tgaaagcggc ttttcacggc ccgcccaata tcgtgaatgt
     2641 tcactcgctt ttcgttcaga aacgaatcgc catcccgcga aatcgtgata accggcagct
     2701 ccttcgccgt gtcctgcgtg gtatccacct tcggaacttc cacttccatc ccgaactcca
     2761 tcacgtgcgc cgtgagcatg aagataatca gcaacaccag cacaacgtcc accagcggaa
     2821 tgatgttgat ctcggcgagc tgcgtgttgc cgcgaaagcg tttgtttcca aaaacgctgc
     2881 cgttcgggga agaagagccg cctacggaga acgccatgcg ctattcctcg aaacttcgct
     2941 cggtcatatt catgaactcg aggccgaagt cttccatgcg ggtaccgaac tccttgatgg
     3001 ttcccccata caaattgtaa gcaatggcgg cgggaatcgc ggcggcaagg ccgagagcgg
     3061 tggtgatcag cgcttcggag atgcccggcg ctaccgcacg caagctggcc gagccggctt
     3121 ggcctaacga agagaaagcg tcgatgatgc ccatgacggt gccaaacagt ccgatgaatg
     3181 gagagacgga ggcggtggtc gccaggatgc ccatgctccg ctccagccgc tcgatctctt
     3241 cgctgatgcc gagttgcagg ctgcgctcga gcgcaagctt gttcgacaca tctccttttg
     3301 ttttgcgctg ccgctcgaat tcgctatagc cgaaatcgaa caccgtcacc agcggcgacg
     3361 cgcggaactg ttcgcttgcc acggcgacag cttcgaggcc aggtgttttg cggaaagctc
     3421 gcaaaaactg gcggttcgca atcttggccc gccggaacag gccccacttc gcgagaatca
     3481 ccgcccatga aaggatcgag aggacgacca ggattaccat cacgaccttg gcaatggggc
     3541 cagcggtctt gacgagttcc cagagactca attgcagaaa aatagcgccg aacagcgttg
     3601 gggtcaatcc agctccgttc ctccttggac agatttcagc aactcttatg ataatcctac
     3661 gcgggcaatt ttacagaaga aaaaaacgga atcggaggat tttgtttcaa aggacgcgct
     3721 gacgcgaccg cgttgacgcc ccaggccgct ggcaaataga agtcacaagc acccgccggt
     3781 tcttgcccat tcgctttcat caatctggga gcgcttcacg aaagcgtccg tcgtctcccg
     3841 tcctattctg gacatggagg gatgaatgaa ctatggagcg cccgccacta taaaaaaacg
     3901 aaacgaaccc aacgcggcct tttggctggc ggcgcggccg cgttgatctc cccgcgcgcg
     3961 cctgtccttg ggaggcctcg ccagcaaata gaagtcacaa gcaccggcgg ttatttccca
     4021 ttcgctttca tgcatctgat ggcgcttcac gaaagcgtca attgtctccc gtcctattct
     4081 ggacatggag ggatgaatga actacggagc gaccgccact ataaaaaaac gaaa
//
LOCUS       DATNDT010000217         6814 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3456051, whole genome shotgun sequence.
ACCESSION   DATNDT010000217 DATNDT010000000
VERSION     DATNDT010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3456051"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..538
                     /locus_tag="VN788_07040"
     CDS             <1..538
                     /locus_tag="VN788_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HXU20318.1"
                     /translation="ALAHGRGVDRDAIAQALATFRSVRRRMEIKGEAGGVLVVEDFAH
                     HPTAIRSTLEAARMRWPKRRIWAALEPRSNTMRRKIFQEALPEALSLADTVLFGPVNR
                     AQLLEKGERLSPEDICELIRARGRNAEACGSAGDIANRIASEATPGDMVLIMSNGGFD
                     GLTAKLLEKLRNPVGARG"
     gene            562..2136
                     /locus_tag="VN788_07045"
     CDS             562..2136
                     /locus_tag="VN788_07045"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20319.1"
                     /translation="MLRIWALVAGTFFGVGAQPASATIAYRISLANPDQHLFHVEMTI
                     PVNGKEVVTALPAWNALYQVRDFSYRVTAVSALCAASTGVTLAARLLDKDTWRISLAG
                     MCQPGDRNSFEIRYSIRWDDSGPFNSQLNDHHAFMNLAEILMYVPDRRAEDTSVTFEN
                     LPAGWRTAAELATGGVPNTYAAASYDSLVDAPVEAGEFDEFQFVESGARFRVVVDATN
                     WKKEALEGALERITRYEMRLMGGPPPFDLPSRGYTFVVHIGPYEDQGGGGMEHRNSTA
                     IAATSVEAAAAVAAHEFFHVWNVKRIRPRTLEPVDYTKEQYTRALWFAEGVTSCYASF
                     VLERSGLWSKKQFYDDLALQISELQSRPAHTWQSAEASSLDAWLEKYEAYNAPDRSIS
                     YYDKGQILGVLLDLAIRDATDNRKSLDDVLRRMNEEYAKKGKFYDDSNGIRATVEEVA
                     GKNFEDFFASYVSGTAEIPYNDFLAAAGLELNGSAAQAADRNVRSSEPSSISEIAHPT
                     DRQRRIREGLLRGTTD"
     gene            2167..2880
                     /locus_tag="VN788_07050"
     CDS             2167..2880
                     /locus_tag="VN788_07050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXU20320.1"
                     /translation="MIRTCLLVLFFAFAIIFGLPLLLLWTVLTGNPEFMYAVAMKAVR
                     FGNRLAGVRVRAVGLENIPAQPCVFIANHVSNIDPLVFIPAIPQRVGILVKRELFRIP
                     IFSSAMIRAQYTPVDRGDRERSGKSISAAARILKDGLSYAVFAEGTRSADGRLRPFKK
                     GGFTMAIEAGAPIVPVSIAGTQRLLQKGSWIIRRGEAKVIFGPAVEASEYTLESRAEL
                     MQRVESLVAASLPPEQQPL"
     gene            2981..3706
                     /locus_tag="VN788_07055"
     CDS             2981..3706
                     /locus_tag="VN788_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXU20321.1"
                     /translation="MAGKLIHEVLPVGLLQCNCSILGDTATGDAIVVDPGDEVERILE
                     IIRRHNLKVRAIISTHTHIDHVGGLATLHKATGAPVMVHEADLTLYANLETQAEWLGV
                     PTPAIMRIRDVVKEGDTVRWGGFAARVLHTPGHTPGSISLLVEPGAPTGVERDVAPAS
                     RRASSREDPPNDALTARRLLAGDTLFHGSIGRTDLPGGSFPEIIKSIREKLLVLPESM
                     VVLPGHGEATTIGFEREHNPFLV"
     gene            3991..4308
                     /locus_tag="VN788_07060"
     CDS             3991..4308
                     /locus_tag="VN788_07060"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU20322.1"
                     /translation="MGWRVRFCQSPAAIQALMEKNLGATAKGKPGSAYHLLTNFMIDV
                     KGDTATAWSRWSFTVTGADKKPSFLYGGHYDDKLIREDGHWRFLRRLAVNDIPHSEPT
                     EAQ"
     gene            4651..5211
                     /locus_tag="VN788_07065"
     CDS             4651..5211
                     /locus_tag="VN788_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20323.1"
                     /translation="MRRSDLVKSPSKGKSVRTSQIVFGERQHLLRVLDSVERSALPTS
                     RLDQERRVIEQLIHARTQELNRINAGWDEKVGVVLSAEVKPEALEQLSRQAPREDYYL
                     LRLISEHPKVAARTLGRLSHHPYSAIRENIARHPNSDAATLSRLSRDRSQPLWYLVAF
                     NPNAPAPLRKKLQERMRRLGEKSATQ"
     gene            5498..5755
                     /locus_tag="VN788_07070"
     CDS             5498..5755
                     /locus_tag="VN788_07070"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXU20324.1"
                     /translation="MSETKPSETQIEQRAYELFLERGCEHGRDIEDWLEAERELTELA
                     ELRTVETELAEEQSFAESEKPTRAKKQAAAAGVAAAVASST"
     gene            complement(6317..>6814)
                     /locus_tag="VN788_07075"
     CDS             complement(6317..>6814)
                     /locus_tag="VN788_07075"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017726944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXU20325.1"
                     /translation="VRLAVAGGRVEILASELVDVEPEDLFPAPPPVNVDFSARYSKFI
                     HAAAQKYSLDEKLIAQVIAAESNFNANAVSRKRALGLMQLLPKTAARYALVNVFDPAQ
                     NIDAGTHYLKDLLHRYRGDVRLALAAYNAGPEMVDRYRGVPPFPETQNYVKQITLNLA
                     LLAAR"
BASE COUNT         1450 a         1856 c         2066 g         1442 t
ORIGIN      
        1 tgcgctggcg cacggccggg gcgtcgatcg cgatgcgatt gcgcaggcgc ttgcgacgtt
       61 ccgaagcgtg cgccggcgaa tggagatcaa aggggaagcg ggcggcgtgc tggtggtcga
      121 ggatttcgcg caccatccta cggcgattcg gtcgacgctt gaggctgcga gaatgcgctg
      181 gccgaaacgc cggatttggg ccgcccttga accgcggtcg aacaccatgc gccgcaagat
      241 atttcaggag gccttgccgg aagctctttc actggccgac accgtgcttt ttggaccggt
      301 aaatcgcgcg caattgctgg aaaaaggcga acgcctttcg cccgaagata tctgcgaatt
      361 aattcgagcg cgcgggcgca acgcagaggc atgcggttcg gcgggcgata ttgcaaatcg
      421 aattgcgtcg gaagccacgc caggcgacat ggttttgatt atgtccaacg gagggtttga
      481 cggcctcacg gcgaagctgc ttgagaaact tcgaaacccg gttggtgcgc ggggatgatc
      541 gatgctggtg gcgcgaagcg catgcttcga atctgggccc tggtggcggg aacgttcttc
      601 ggagtcgggg cgcagccagc ttccgcgacg attgcctatc gcatttcgct cgcaaatccc
      661 gatcagcacc tttttcatgt ggaaatgaca attcccgtga atggcaagga agtcgtaacg
      721 gcgcttccag catggaacgc gttgtatcaa gtgcgtgatt tttcctatcg cgtgacagcg
      781 gttagcgcac tctgcgccgc atcgactggc gtcactcttg ccgcgcggct gctcgataag
      841 gacacttggc gcatttcgct tgcgggtatg tgccaaccgg gcgatcgcaa tagctttgaa
      901 attcgctact ccattcggtg ggacgattcc ggccctttca attctcagct caacgatcat
      961 cacgccttca tgaatcttgc tgaaattctg atgtatgttc cagaccgacg ggctgaagat
     1021 acgtccgtta cttttgaaaa tcttccggcg ggctggcgaa cggcggcgga attggcgacg
     1081 gggggcgttc ccaatacgta tgctgcggcg agttacgatt cgctggtgga tgcaccggtc
     1141 gaggcgggcg agttcgacga atttcaattc gtggaatccg gcgctcgctt ccgggtggtg
     1201 gtggatgcca cgaactggaa gaaagaggcg ctagaagggg cactggagcg gattactcgc
     1261 tacgaaatga ggctgatggg tggcccgcct cccttcgatt taccgtcgcg cggatataca
     1321 tttgtcgttc acatcggccc ctacgaggat cagggtggcg gaggcatgga acaccgaaac
     1381 tcgacggcca tcgcagctac gtccgtggag gcagccgcgg cggtcgcagc gcacgaattt
     1441 tttcatgtgt ggaatgtgaa acgcattcgc ccgcgcacgc tcgaaccggt ggactatacg
     1501 aaggagcagt acacgcgggc gctttggttc gcggaaggag tgacgagctg ctacgcgtca
     1561 ttcgtcctgg agcgttcggg gctttggtcg aagaagcagt tttacgacga tttggcattg
     1621 caaatcagcg aactgcaatc gcgcccggct cacacgtggc aaagcgcgga agcctcgagc
     1681 cttgacgcat ggctggagaa atacgaggct tacaatgctc ccgatcgcag catttcgtac
     1741 tacgacaagg gccaaattct gggagtgctg ttggacttag ccattcgcga cgccacagat
     1801 aatcgaaaat ctctcgacga tgtgctgcgg cgcatgaacg aggagtatgc aaagaaaggc
     1861 aagttttacg acgatagcaa tgggattcgc gcgacggtcg aagaagtggc gggaaaaaac
     1921 ttcgaagatt ttttcgccag ttacgtctcc ggcaccgccg agataccgta caacgatttt
     1981 ttggcagcgg cgggcctgga actgaatggg agcgccgctc aagcggcaga tcgaaacgtg
     2041 cgatccagcg aaccttcttc aatttcagaa atcgcgcatc ccaccgatag gcagcgacgc
     2101 atacgcgagg gcctgctgcg cggcaccacc gattgacatg cgcttcacac cgggattttt
     2161 tgctgtatga ttcgcacatg cctactcgtt ctctttttcg cattcgcgat cattttcggt
     2221 ttgccgttgc ttctgctttg gaccgttctg accggcaatc cggaattcat gtacgcggtt
     2281 gcgatgaaag cggtgcgctt cggtaaccgg cttgcgggcg ttcgcgtgcg cgcggtcggc
     2341 ctcgaaaata ttcccgctca accttgcgtg ttcatcgcca accacgtctc caatatcgac
     2401 ccgctcgtat ttattccggc gattccgcag agagtcggca ttctagtgaa gcgggagcta
     2461 tttcggattc ccatcttctc ttcggcaatg attcgggcgc aatatacgcc cgtggaccgc
     2521 ggcgatcggg aaaggtctgg aaagagcatc tccgctgcgg cgcgtattct gaaggacgga
     2581 ctttcctacg cggttttcgc ggaaggcact cggagtgcgg atgggcggtt gcgcccgttt
     2641 aagaagggcg ggttcacaat ggcgatcgaa gcgggcgcgc caatcgtgcc ggtctccatt
     2701 gctggaacgc aaaggttgtt gcaaaaaggg agttggatta tcagacgcgg cgaggcgaaa
     2761 gtgatattcg gaccggccgt ggaggcgtcc gaatacacgc ttgagagccg cgcggagttg
     2821 atgcaacgcg tggagtcgct tgtggccgct tccctgccgc cggaacagca accgctttag
     2881 gaaactatcg gcatcgggcc ttcgcgaaac acaaccatcc atcctgcatc ttaagccgca
     2941 aagccgaaga tttatctccc gcttgctata ctgctttgcg atggccggaa aattgattca
     3001 cgaggtattg cccgttgggc tgctgcagtg caactgctca attctgggcg acacggcgac
     3061 cggcgatgcc atcgtagttg atccaggaga cgaagtcgaa cgtatcctcg aaattatccg
     3121 acgccacaat ctgaaagtgc gcgcaatcat cagcacgcat actcatatcg atcatgtggg
     3181 cggtctggcc acgcttcaca aggcgaccgg cgcaccggta atggttcacg aagcggatct
     3241 tacactttat gcgaacttag aaacgcaggc ggagtggctt ggtgtaccca cgccggcgat
     3301 aatgcgcatt cgagatgtcg tgaaagaggg agacacggtg cgctggggcg gcttcgcggc
     3361 gcgcgtgctg cacacgcccg gccatactcc gggaagcatc agtctgctgg tcgaaccagg
     3421 ggctccgact ggcgtcgaac gggatgtcgc gccggcgtct cgccgcgcct ccagccgcga
     3481 ggatccgccg aatgacgcgc tcactgcgcg gcgccttcta gctggcgaca ctttatttca
     3541 tggcagcatc gggcgcaccg atttgcccgg agggtccttt cccgaaatca tcaagtctat
     3601 ccgcgaaaag ctgctggtcc tgccggagag catggttgtt cttccagggc atggcgaagc
     3661 gactacgatc ggcttcgagc gggagcacaa tccttttttg gtgtgagaag acacatcgat
     3721 ggcgtctgcg cggagcagat ttgaagttcg ccaatcaggg tgatattgtt cgccgcgttt
     3781 cgcgtattcc gcgagaaagg caattcggaa atgaagaact cggcgattct tatcttttcc
     3841 caggccgccc gcattgctgt tgtgccttga agcgcgaatt cagcggcttg aagatatcca
     3901 ggagatacgc gatctattaa cgagctatgg gcgcttgctc gacgctcacg acctggctgg
     3961 atattcgcga ttgttcgcca aaaacgggga gtgggttggc gggttcggtt ctgccaaagt
     4021 cccgcggcca tccaagcctt gatggagaag aacctgggcg cgactgcgaa ggggaagccc
     4081 gggtccgcat accacctgct cacgaatttc atgattgatg tgaaaggcga tacggccacg
     4141 gcctggtcgc gctggtcgtt tacggtgacc ggcgccgaca agaagccctc cttcctgtac
     4201 gggggacact acgatgacaa actcatccgc gaggacggcc actggaggtt cctgcgccgg
     4261 ctggcggtga acgacatacc gcattcggag ccgaccgagg cccaatagcc gggtccacct
     4321 cgctgtgctt ggatggaaat cgcggtcggg cgctggttct aggaccctct gttgcggggt
     4381 acggaaactc ggccaccaac tttgctttac tgttggatac ggcgacagga taatcaccgc
     4441 ggtcgtccat agctggcagc gctgactgag tgtattcctt ggggagacac ggttagcgag
     4501 ggcaaacatg tcactttccg ctatacttac tttacgcgac gcgagatgcc gccagatttt
     4561 gtgtccatat tccggcggag cgtaaaggtc gtttccctgg cttagcgccg ccgtcgcggc
     4621 gacccatgaa atcttcgggg gtatttagaa atgagacgtt ccgacttggt aaagagtccg
     4681 agcaagggga aatctgtgcg cacatcacaa attgttttcg gcgagaggca gcatttgctg
     4741 cgagtactgg actcagtcga gcgcagcgct ttgcctactt cgcggctgga tcaggagcgg
     4801 cgcgtgatcg agcagttgat tcacgcgcgt acgcaagagt tgaaccgcat caacgcggga
     4861 tgggatgaaa aagtgggcgt ggtgctcagc gccgaagtga agccggaggc gctcgagcaa
     4921 ctttcgcgcc aagcgccgcg agaggattac tatctgctcc gcttgatcag cgagcatccg
     4981 aaagtggccg cgaggacact gggccgtctt tcgcaccatc cctattcggc gattcgcgaa
     5041 aacatcgcgc ggcatccaaa tagcgacgca gccacgcttt cgcgcctgag ccgggatcgc
     5101 tcgcagccgt tgtggtatct cgtagccttc aatcccaatg ctcctgcgcc actacgcaaa
     5161 aagctgcagg agcgcatgcg gcgcctgggc gaaaaatccg ccacgcagta ggaaaccatt
     5221 tcgagcgcct agggcatttg ggcccgtctc gttttgtgtc ggtagagtgt gccaccaatc
     5281 ctctgtttgc aatcgcgttc tattcggctg gcacgtcgag tagacgttca cgcgcatctt
     5341 tggactcctg catagacggc ttcgtatcca gcgagcacgc gcggcggccg gcgctcctta
     5401 ccgggttgtg aaaaatacaa acaacacccg atggcccatc gctggcagcg ggggcaaact
     5461 ccgtgtcgca ccaggttttg ccaaggagct aagtatcatg tctgaaacca aacccagcga
     5521 gacacagatc gagcagcgcg cctacgagtt gtttctggaa cgcggttgcg agcacggaag
     5581 agatatagag gactggcttg aggccgaaag agaactgacc gaactcgcgg aattgaggac
     5641 ggtcgaaacc gagctcgcgg aggagcaatc attcgccgaa tcggaaaaac cgactcgcgc
     5701 gaaaaagcaa gctgcagcgg cgggcgttgc cgcggccgtc gcgtcttcca cgtaaggcaa
     5761 gttccccact tttttggcca cagccaagcc cggtctccga ggctcgggct gtggcttgct
     5821 ttttcgggct cgcccaggat acccagcgaa tcctagctac ggccatgcgt ggtcccgttc
     5881 ctctgaacgt gagttgaggc tacggttctg aaccatccgg aaatgggatc acgaattttc
     5941 ccacgaagct atcgtagatt gcgtagtttc cagaatgctt gacgacaagt tcctgaaaac
     6001 gtttccgggc ttcttccaaa gtcgccgatg tttcgatcca caggtgacgg ccatcgggta
     6061 gtttttggaa tatgtcaaac gaccgataag gcatgttctc cccttagaaa gagctaaacc
     6121 catcggttgc gggggcagtg tgtatagcag agacgaggtg caaagtctgt tggcccaccg
     6181 acagagcgca gccaagccct tccacgcgat caacacttcc acggtaaaga ccaaagccca
     6241 cggcgagaag tgcgctcatt tagctaacgt attactcagc cagatgcgcg tactgagctc
     6301 cggctgtgcc gtgcagctac cgtgcggcga ggagagcgag gttcaaggta atttgtttca
     6361 cgtagttttg tgtttccgga aaaggaggga cgccgcgata gcggtccacc atctcgggac
     6421 cggcattgta ggcggcgagg gcgagccgaa catcgccgcg gtagcgatgg agcaaatcct
     6481 tcaagtagtg agtgccggcg tcgatattct gcgcggggtc gaacacattc accagcgcat
     6541 aacgcgcggc agttttaggc aacaattgca tcaggccgag agcgcgcttc ctcgatactg
     6601 cgttggcatt gaaattcgac tcggccgcaa ttacctgggc gataagtttt tcgtccaaac
     6661 tatatttttg cgcggccgcg tgaatgaatt tcgagtaacg cgcagagaag tcaacattca
     6721 cgggaggagg agcgggaaaa aggtcttccg gttcaacatc gaccagttcg cttgctaaga
     6781 tttcaacgcg gccgcccgcg acggccaacc gcac
//
LOCUS       DATNDT010000218         9161 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2612356, whole genome shotgun sequence.
ACCESSION   DATNDT010000218 DATNDT010000000
VERSION     DATNDT010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9161
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2612356"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..191
                     /locus_tag="VN788_07080"
     CDS             <1..191
                     /locus_tag="VN788_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20326.1"
                     /translation="GTLLLNDGFNVAIQGKDGWYHSWPRSDVKLEVHDPLAAHAALLQ
                     KYTDADMHNIFTYLETLK"
     gene            188..1777
                     /locus_tag="VN788_07085"
     CDS             188..1777
                     /locus_tag="VN788_07085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acido-empty-quinoprotein group A"
                     /protein_id="HXU20327.1"
                     /translation="MKKLFALLVFMFAFAVCLPAQELDPSQLLKPPTNDWPTFNGDYT
                     GRRYSPLTQINKDNVGTLALAWAFQSHSQGLQSMPLEVNGILYISGGNQIWAADARTG
                     RQIWHFVRPGFPPGGGNKGVAMWKNRLYMNTFDAQLLCVDAQTGKMLWQIQIADPALK
                     AFGTVAPLVVGNHLIEGTSGDTADLPGFLESINPVDGKIEWRWDSMPKQGDPAWNTWP
                     HDTDVITRGGGMTWLTGTYDPELNLLYWGTGNPHPVENGYTRVGANLYTCSIVALNPD
                     TGKLVWYFQSSPHDTHDWDTVMTPVLFDGTFKGKPRKMLAQANKNGMFFVLDRTNGQA
                     LLSEPFVPANWFSGFNKRGEPIPAKDKEPQPDGVLVSGGVGTNWQAPSFDAQTGLFYV
                     NSRESMGVFYLTMPGKNAEGWAGRDFFLSSKSMLKAIDYQTGKTRWEHTANGRGGQSG
                     ILTTAGHLLFTADDAQNLVALDPVTGKTLWHMYPGGGLGTGPITYELDGRQYVVFPVD
                     GVVYGFALPKAPAANSRDLGQ"
     gene            2011..3132
                     /locus_tag="VN788_07090"
     CDS             2011..3132
                     /locus_tag="VN788_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXU20328.1"
                     /translation="MSNFVKWLAMATVGLGLVVFAPRMTAAQDDLGQQGFGDPSAAAA
                     NLPIDFIKPADVEKALADKTAKFVLVDTQPAEAFAEGHIPGAINFPWVPKVTPPISLP
                     RFKELVFYCPCNHDEDSIDMVKKLREFGYLDSKVLEGGWYKWVALKYPVDGTDAANAI
                     KEADAAAASGANSSNAGNSANASTPANTAAPAATSAATETAATSSGPITSGRPVGAVT
                     PSFRVIDVTGKYKGQDTCYVCEYGEAPTVIAFFNNPSDQAADLIVKLNDLAKKESSKN
                     FKAFVVMVNGPDSKPWLEKLAQDKGIQIPMVYLARGTKDLGVRLYKINPQAKNTFLVD
                     DKRQVVTNLVNVDDFQKVADASEKMLASAGSTTSGSGGQ"
     gene            3129..3692
                     /locus_tag="VN788_07095"
     CDS             3129..3692
                     /locus_tag="VN788_07095"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXU20329.1"
                     /translation="MRSPFRVTSLKPFALGLAALALLAISPLAVSAPSAAPADTREMK
                     VVQPQDVHALIAANKGKVVFLNFFATYCVPCHTEFPDIIKMARKYQNAGVQVVEVSMN
                     DNSDPSDKGAMLKWLGETNPPFPVYIASSLEDDFYKGVSPQWAKDGEGLPMTMIFDRD
                     GKMVHYYQRALTLAEMESDVTPLLAAK"
     gene            3880..5613
                     /locus_tag="VN788_07100"
     CDS             3880..5613
                     /locus_tag="VN788_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU20330.1"
                     /translation="MRYSPLSQINKPNVAKLHRAWTFHTGDPAADVNSEGAPLVIDGV
                     MYLEAGKNVFALDPVTGRQIWKFETKGTNRRGLSYWPGDKQTPPRLILGVADDKMLAL
                     NAKTGKPASGFGEGGFVSGVSPASAPVIYHDLIITGQNRVQSVRAFDAHSGKLVWTFY
                     TKGQPGEPGHDTWKDNAWDVRAGNDVWSFMTLDPQRGIVYMPIPPTGGPDFYGGGRLG
                     DTLYSDSVVAVHANTGKLIWYQQLVHHDLWDWDVPGAPTLFDVKRNGKTIPAVAEFGK
                     QAYLFLFDRTNGKPLFGMQERPVPQTTVPGDATSPTQPVPLKPPALAPNSISRSDIYN
                     LTPEHAAFCRNLWDANHMYNDGPFTVYSTDPNKMAVIFPGREGAGNWSGIAFDPKLDY
                     IFGTTTFNAGQIGRLVKLDKPAPGPFGGPYDKVFPNGPSRYQSRFWEPSNGWPCIKPP
                     WAEIFAVNAKTGDIVWHVPFGTVDALAAKGFPDTGAPNTGPMVVTAGGLIFIGATNDG
                     RFRAFDARTGKELWAEKIDASAHTIPITYQGRDGKQYVVVEAFGGPGIVALNGYFNDT
                     PGDSVIAFALP"
     gene            5746..6870
                     /locus_tag="VN788_07105"
     CDS             5746..6870
                     /locus_tag="VN788_07105"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU20331.1"
                     /translation="MEKKSPIKSAIQGFRYSIVGPRTLLWLSLGFLPLAVASSALAQS
                     AQPSTAAEAQSSKSSAPETGASGAPQQPDQQSLATISGTIVDQTGAAVAGAQVTLTRE
                     GQSAKQETTSGEDGQFAFDNVAPGPFQLTVSAPDFATQTSSGILQSGEARIVPPISLI
                     LAAATTQVRVGLTQTEIAEVQIQTEEAQRVLGFIPNFYVSYVPDAVPLSSKQKFQLAG
                     RSLLDPVTFGVTAAVAGMEQEANIFSGYGHGPEGYAKRFGAAYTDRATSTFIGSAVLP
                     SLLKQDPRYFYEGTGSKRSRLLYAIKTTFICKGDNGRWQPNYSYIMGDFASSGISNLY
                     YPAQERGIGLTFANAFIGIGGTAAANILQEFVIRKLTPHS"
     gene            complement(7044..8054)
                     /locus_tag="VN788_07110"
     CDS             complement(7044..8054)
                     /locus_tag="VN788_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20332.1"
                     /translation="MTRYGILAVAVLALGMSTNAQSSPSNQVNASAQAQTNASAAASA
                     NASRPSDQGTSANKQNSKTSQSRSASASGSSSSSASASANGDSLNIASGTPIEATLVT
                     PVDAKRNKPGDPVVAKTTQDVKQNGQTVLKKGSRLHGHVTQAQARSKGNSESAVGMVF
                     DSAVMKNGQTVPMNLSIQALAASANETSADLAGDQGAIDSMSQTGAGFAGARPAPGGS
                     GVVRGTGGAVGGVGGTVRGVSNVAGGATSSASAVGGAAGNVGQTAGGTLSATTRSAGS
                     VGGLNTAGQLASGSSGVFNLQGLNLTSAASNGTTASVVNSTSRNVHLDSGTQMVLQVV
                     RQ"
     gene            complement(8364..8795)
                     /locus_tag="VN788_07115"
     CDS             complement(8364..8795)
                     /locus_tag="VN788_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20333.1"
                     /translation="MRVSRMVVVCVGLMFFVAPTLLAKGSPSYDKGTLLSMDSTSCGY
                     AEKDGKTITGEILGTDAAHKNTQEVLCQEYLLQSDRMIYRVRPKDSKHPVLLPVGESI
                     DFRIHKNVLLLRDPEGDKKEREYIVVSIQPRQDVKEARNSH"
BASE COUNT         1985 a         2813 c         2401 g         1962 t
ORIGIN      
        1 aaggcacgct cctgctgaac gacggcttca acgtcgccat acagggcaaa gacggctggt
       61 atcactcctg gccgcgcagt gacgtgaaac tcgaagtgca tgatccgctc gccgcgcatg
      121 ccgcgttatt gcaaaaatac acagacgccg acatgcataa catctttacc tatttggaga
      181 cgctgaagtg aagaagcttt ttgctctgct tgtatttatg ttcgccttcg ctgtgtgctt
      241 gccggcgcag gaactggatc catcccagct cctgaagccg ccgaccaacg attggcccac
      301 atttaatggc gactatacgg gacgccgcta tagcccgctc actcagatca acaaggacaa
      361 tgtaggcacg ctcgctctcg catgggcctt ccaaagtcac tcgcagggtc tgcaatccat
      421 gccgcttgag gttaatggca ttctctatat ttcaggtggc aatcagatct gggctgcgga
      481 cgcgcgcacg ggccgccaga tttggcattt tgtacgtcct ggttttcctc cgggtggagg
      541 caataaaggc gtcgcgatgt ggaagaatcg cctctacatg aacactttcg atgcgcaact
      601 tctctgcgtc gatgcgcaaa cgggaaagat gctctggcag attcagatcg ccgatccagc
      661 gctgaaagca ttcggcacag tggccccgct cgtggttggc aatcatctga tcgagggaac
      721 ttccggcgat accgcggatc ttcccggttt cctcgaatcc attaatccgg tagacggcaa
      781 aattgagtgg cgttgggatt ccatgcccaa gcagggcgat cccgcgtgga acacctggcc
      841 gcatgatacg gacgtgatca ctcgcggcgg cggtatgact tggctgacgg gcacgtacga
      901 tcctgaactc aatttgcttt actggggcac tggcaatcct catccggtgg aaaatggcta
      961 cacgcgcgtg ggcgcgaatc tttatacctg ctcgatcgtt gcgctcaatc cggacactgg
     1021 aaaactggtt tggtattttc aaagctcgcc gcacgatacg catgattggg atacggtgat
     1081 gacgcccgtt ctctttgacg gaaccttcaa gggcaagccg cggaaaatgc tcgcgcaggc
     1141 caataaaaat ggaatgttct tcgtgctcga tcgcaccaac ggccaagctc ttttgtctga
     1201 gccattcgtg ccggccaact ggttctcggg tttcaataaa cgcggcgagc cgattcctgc
     1261 aaaagataaa gagccgcagc ctgacggcgt tttggtcagc ggcggcgtcg gcacaaattg
     1321 gcaagccccg agcttcgatg cccaaactgg acttttctac gtcaactcgc gggagagcat
     1381 gggagtcttt tatttgacca tgccaggcaa gaacgccgaa ggatgggcgg gccgcgactt
     1441 tttcctctcc tcgaaatcca tgctgaaggc tatcgactat caaaccggca aaactcgctg
     1501 ggagcatacc gccaatggcc gcggcggaca gtctggcatt ctcaccaccg ccggacatct
     1561 tctcttcact gccgacgatg cccagaatct ggtggccctt gatccggtca cgggcaagac
     1621 cctttggcac atgtatccgg gcggtgggct gggaactggc cccatcactt acgaactgga
     1681 cggtcggcag tacgtcgtct tcccagtcga cggagtcgtt tacggtttcg ccctgccgaa
     1741 ggcacccgcc gccaattctc gagatctcgg ccagtaaggc gcccgccatc tcgctgaaac
     1801 cgagtctgtt cagccacgtc taagtatatg taggctagat gttgggttgc ggctacatgg
     1861 tggatggctc ggaacgcggt attgacaccc tttcgggccg tgagtaacat ccacagttag
     1921 aagcggatag tggagcgaat gggcgcaccc tttgatagcc ggccgaatct aatttagtgg
     1981 ggcggcgcaa ctcaatttcg ggaggttatt atgagcaatt tcgttaaatg gcttgcaatg
     2041 gcgacagtgg gactgggtct cgtcgtcttc gcgcccagga tgaccgctgc tcaggatgat
     2101 ttggggcagc aaggttttgg ggatccctcc gccgcagcgg ccaatcttcc catcgatttt
     2161 ataaagcctg ccgacgtgga aaaggctctc gcggataaaa ctgcgaaatt tgttttggtg
     2221 gatacgcagc ccgctgaagc cttcgccgaa ggccacatcc cgggcgcgat taatttcccc
     2281 tgggtgccaa aggttacccc tcccatctct ttgccgcgct tcaaagagct cgtgttctac
     2341 tgcccttgca atcatgatga agattcgatt gacatggtca agaagctgcg cgaattcggt
     2401 tatctcgatt cgaaggttct cgaaggcggc tggtataaat gggttgccct gaaatacccc
     2461 gtcgacggca ctgacgcagc caatgccatc aaggaagctg acgccgccgc agctagtggc
     2521 gcaaactcca gcaacgcggg caattccgcg aacgcttcga ctcctgcgaa caccgcagct
     2581 cctgcggcga catccgcggc caccgaaaca gcagccacat cgtccgggcc tatcacttca
     2641 ggtcgcccgg tcggcgccgt cacaccgtca ttccgcgtaa tcgacgttac cggcaagtac
     2701 aagggacagg acacctgcta cgtctgcgaa tatggcgaag cacccacagt gatcgccttc
     2761 ttcaataatc ccagcgacca agccgccgat cttatcgtca agctcaacga cctcgccaag
     2821 aaagagtcgt cgaagaattt caaggctttc gttgtgatgg ttaatggccc ggattcgaag
     2881 ccttggcttg aaaaactggc gcaggacaag ggcattcaaa tcccgatggt ctatctggca
     2941 aggggcacca aagacctggg cgttcgtctc tacaaaatca acccgcaggc caagaatacc
     3001 ttcctggtag acgacaagcg ccaagtggtt accaatctcg tgaacgtcga cgattttcaa
     3061 aaggtcgccg acgcttccga aaaaatgctg gctagcgccg gatccacaac ttcagggagc
     3121 ggcgggcaat gagatcgcct ttccgcgtaa cgtcgctaaa accgttcgcg ctggggctgg
     3181 cggcgctggc acttctggcg atcagcccac tagcggtctc tgcaccatca gccgcccctg
     3241 ctgatacccg ggagatgaaa gtcgtccagc ctcaagacgt tcacgccctc atcgccgcaa
     3301 acaagggaaa agttgtattc ctaaacttct ttgccaccta ctgtgttccg tgtcacaccg
     3361 aatttccgga catcatcaag atggcgagga agtatcaaaa tgcgggcgtt caagtcgtgg
     3421 aagtttcaat gaatgataat tccgatcctt ccgataaagg ggccatgctt aaatggctgg
     3481 gcgagacgaa ccctcccttt cccgtctaca tcgcgtcttc gctcgaagat gatttttaca
     3541 aaggagtctc gccacagtgg gctaaggacg gtgaaggcct tccaatgacc atgattttcg
     3601 atcgggacgg caaaatggtc cactattacc agagggcgct tacactcgcg gagatggaga
     3661 gcgacgtaac tccgcttctc gccgccaaat aagaccggca ttcagcgaat gcccccggga
     3721 agattctcac aaactcaggc aagcgctcgt caatcgagca ttgccggttg cctgctcctt
     3781 ttaggcgttc tattcttccc cgtcgcgctc ccggcgcaaa aggatcttcg ggcgaattcc
     3841 caaaatgact ggcgctatgc cggtcacgat ccaggcgcta tgcgttattc ccctctctcc
     3901 caaatcaaca agcccaatgt cgccaagctt caccgcgcat ggacattcca cactggcgat
     3961 ccggccgccg acgtaaattc cgaaggcgct ccactggtca tcgatggcgt gatgtatctc
     4021 gaggccggca aaaacgtctt cgcactcgat cccgttaccg gccgtcaaat ttggaaattc
     4081 gaaactaaag gaacgaatcg gcgcggcctc tcatattggc ccggcgacaa gcaaacgccg
     4141 ccgcgcctca tcctcggcgt ggcagacgac aaaatgctcg cgctcaacgc caaaaccgga
     4201 aagcccgctt ccggtttcgg cgaaggcggc ttcgtgtccg gcgtcagccc cgcttcagcc
     4261 cccgtcattt atcacgatct catcatcacc gggcaaaatc gcgtgcagtc cgttcgcgct
     4321 ttcgacgcgc actccggcaa gctcgtttgg actttctaca ccaagggtca gcccggcgaa
     4381 ccgggtcacg atacttggaa agacaacgcg tgggacgttc gcgccggcaa tgatgtctgg
     4441 agttttatga ccctcgatcc ccagcgcggc atcgtttaca tgccaattcc gccgaccggc
     4501 ggtcccgatt tctacggcgg aggccgcctc ggcgacacgc tttattccga cagcgtcgtc
     4561 gcggtccacg ccaacaccgg caaattgatc tggtatcagc agctcgtcca tcacgacctt
     4621 tgggattggg atgttcccgg cgcgcccacg ctctttgacg taaagcgcaa cggcaagaca
     4681 attcccgccg tcgctgaatt tggcaagcag gcctacctct tcctcttcga tcgcaccaat
     4741 ggcaagccgc tcttcggcat gcaagagcgt cccgtgccgc aaaccaccgt ccccggcgac
     4801 gctacttctc cgacacagcc cgttccactg aagccgccgg cgctcgcgcc caatagcatc
     4861 tcgagaagcg acatctacaa tctcacgccc gagcatgccg ccttctgccg gaatctgtgg
     4921 gacgcgaatc acatgtataa cgatggacct ttcaccgttt acagtaccga tcccaataaa
     4981 atggcggtaa ttttccccgg ccgcgagggc gccggcaatt ggagcggcat cgcgtttgat
     5041 cccaaactcg attacatctt cggcacgacc acgttcaacg cgggccagat cggccgcctc
     5101 gtaaaactgg ataagccggc tcccggtcca ttcggcggcc cttacgacaa ggtatttccc
     5161 aacggccctt cgcgctacca aagtcgcttc tgggaaccga gcaacggctg gccctgcatc
     5221 aaaccaccct gggccgaaat attcgccgtc aatgccaaaa ccggcgacat cgtctggcat
     5281 gttcccttcg gcacggttga tgcgctcgcc gcgaaaggat ttccggatac tggcgcgccc
     5341 aatacgggcc caatggtcgt caccgccggc ggtctgatct tcatcggcgc cactaatgac
     5401 ggccgctttc gcgccttcga cgcccgcacc ggcaaagaat tgtgggccga gaagattgat
     5461 gccagcgccc acaccatccc cattacctac caaggccgcg acggcaagca atatgtcgta
     5521 gtcgaagcct tcggtggtcc cggcatcgtg gccttgaacg gttacttcaa cgatactccc
     5581 ggcgacagcg tcatcgcctt cgcgctccca taacataaaa ttgttctaga ttcgtagcgg
     5641 cggtcatcac gcggacaatc tatctgtctc cagacgacat cagattatca gtgtggtatg
     5701 cagtcggttg aatctaggac caagtaaaca caagctgtga actcgatgga aaaaaagtcg
     5761 cctataaaat ccgcgatcca gggcttccga tactcgatcg ttggcccgcg aactttgctg
     5821 tggctaagcc tgggcttcct gcctcttgcc gtggcatctt ccgccttggc gcaatctgcc
     5881 cagccttcta ctgcagcgga agcgcaaagt agcaaatcct ccgcgccgga aacgggcgcg
     5941 tcaggtgccc cgcagcaacc cgatcagcag tcgttggcga ccatcagcgg cacgattgtg
     6001 gaccaaacgg gagccgccgt cgccggtgct caggtaactc tcacgcggga gggtcagtcc
     6061 gcaaagcaag aaacgacgtc aggcgaagat gggcaattcg cgtttgacaa cgttgctccc
     6121 gggcctttcc agctcacggt atcagccccg gacttcgcga cgcaaacatc ctccggcatc
     6181 ctgcaatctg gcgaggcgcg cattgttccc cccatcagct tgattcttgc tgccgccacc
     6241 acgcaagtgc gggtcggact cacgcaaacc gaaatcgccg aagtgcagat tcaaaccgaa
     6301 gaggcgcagc gcgtcctcgg ctttattcca aatttctacg tgagctacgt ccccgatgcc
     6361 gtccctctct cctcgaagca gaaatttcag ctcgccggga gatcattgct cgatccggtc
     6421 accttcggcg tgactgccgc cgtcgcggga atggagcagg aagcaaacat tttcagtgga
     6481 tatgggcacg gcccggaggg ttatgccaag cgtttcggcg ccgcctacac tgaccgcgct
     6541 accagcacgt tcattggaag cgctgtcctg ccgtcgctcc tgaaacaaga tccgcgctat
     6601 ttctatgagg gaaccggtag caagcgatca cgccttctgt atgcgattaa aaccactttc
     6661 atctgcaaag gcgacaacgg acgctggcag ccaaattact cgtacatcat gggagatttc
     6721 gcctcgagcg gtatctcgaa tctttattac ccagctcagg aacgcggaat cggcttaaca
     6781 ttcgcaaacg ctttcatcgg aatcggtggc accgccgccg ccaatatcct ccaggaattt
     6841 gtcatccgca agttgacgcc ccattcctaa ccagccacgc ctagggtcgg tcactaaaat
     6901 ctatgcctac catcggacgt cgcggatggt tctcgttaaa atgaggtcgc ctggaattgc
     6961 atcttgggaa atcgccgagt ggtaagattt ccagccgtgc ggtgccggaa aagaccccgc
     7021 acggccggat cgtgcaaggg gatctactgt cgcaccacct gcagcaccat ctgcgtgccg
     7081 ctatccaagt ggacgttgcg actcgtcgag ttcaccaccg aagccgtcgt tccattgctg
     7141 gctgcggacg tcagattgag tccctgcagg ttgaacacgc cgctgctgcc cgacgcgagc
     7201 tgacccgctg tgtttaggcc tccgacgctg cccgcggacc gtgtcgtcgc actgagagtc
     7261 ccacccgctg tctgtccaac attgcccgca gcgccgccca ccgcactcgc ggaactcgtc
     7321 gcaccgcccg caacgttgct cacgccgcga acagtcccgc cgacgccgcc aacagcgccg
     7381 cccgtcccgc gaacaacccc gctgccgcca ggcgccggtc gcgctcccgc gaatcctgca
     7441 cccgtctggg acatggaatc gatcgcgccc tggtcaccgg cgagatcggc cgaggtttca
     7501 ttcgccgacg cggccagtgc ctgaatcgag agattcatcg gcaccgtttg cccgttcttc
     7561 atcaccgcgc tgtcgaaaac cattcccacc gccgattccg agtttccctt cgagcgtgct
     7621 tgcgcctgcg tcacgtgccc gtgcaggcgc gaacccttct tcaggacggt ctggccgttc
     7681 tgctttacgt cctgagtcgt cttcgccaca accggatcgc ccggcttgtt gcgcttcgca
     7741 tccaccggtg tcaccagcgt cgcctcaata ggcgttcccg atgcaatgtt caaggaatcg
     7801 ccgttcgcgc tggccgatgc tgaactcgaa ctactgcctg aggccgacgc cgatctcgat
     7861 tgggacgttt tcgagttttg cttgttcgct gatgttccct gatcgctcgg acgcgaggcg
     7921 tttgccgaag ccgctgccga cgcatttgtc tgggcctgtg ccgacgcgtt gacctgattg
     7981 ctcggcgaac tctgagcatt cgtcgacatt ccaagggcca gcactgctac cgcgagaatt
     8041 ccgtaacgtg tcatctccac tcctcccatt gtcctatgga ctgataagta ccctcagtta
     8101 cccaagtctc ctgacaaatt ttgttaggta cttcttccag accctcagat aggcccacaa
     8161 aatgcaatcc gaacgccaag acgcaacagg tttcttttga atcgcttgaa agctgtctca
     8221 tccaaaaatg cccacgctct cttgagtcaa aaagcaccgc acccggtaga agttgcctgg
     8281 gtagcgcccg gcaccgaggt ggcaaaaaac gacaacaact gcaggcgtaa ctgcctcaag
     8341 gttttcgaac gaacttcgca aatctaatgg ctgtttctcg cttcctttac gtcttgccgc
     8401 ggttgaatcg acaccactat gtactcgcgt tctttcttgt cgccctcagg atcacgcagc
     8461 aacaacacat tcttgtgaat cctgaaatcg atagactcac ccacaggcaa taacaccgga
     8521 tgcttcgaat ccttcggccg cacgcgataa atcatgcgat ctgattgcaa cagatactcc
     8581 tggcagagaa cttcctgcgt attcttgtgt gccgcatcgg ttcccaggat ttcgcctgtg
     8641 atcgtttttc catccttctc ggcatatccg caggacgtcg agtccatcga tagcagcgtg
     8701 cccttgtcat agctgggtga gccttttgcc agcagcgtcg gagctacaaa aaacatcaag
     8761 ccgacgcaaa caaccaccat acgcgacact ctcattgtgt tcatctccgt tgtttggcga
     8821 cggaaccaac ctgctggata gtcagttgga gtcgcgcttt tttttaaaga tgcgtctcgg
     8881 tgatttaagg gcaattccga ttccagcgcc taacttacct gctatcaaat cgttgcgatg
     8941 atggcgaacg ggctactgta aagcgccggg gtaaaagcgt aacaattcgt gtagtcgcca
     9001 cgccctgata ctccagctcc ccaacttgcc gttgacgcgg cggagaggta ggctcgaaca
     9061 gagagattca aggcggaagt tggcgatgaa aggcacactc gctggaaatc agattcaccc
     9121 ggactctgcc gccgagctga ggcgccatct cttctacgag a
//
LOCUS       DATNDT010000219         3710 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3030627, whole genome shotgun sequence.
ACCESSION   DATNDT010000219 DATNDT010000000
VERSION     DATNDT010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3030627"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..1483
                     /locus_tag="VN788_07120"
     CDS             275..1483
                     /locus_tag="VN788_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXU20334.1"
                     /translation="MSYSKVVRTIAVSAALIAAGAASAAAQQVQTVFVIALENHNWTQ
                     PDNGPTARLHQIYKNAAAPFINSLVDGTATATIDGKQVDISKQVAYAAAYHNALATAN
                     GDNTHVHPSEPNYIWAEAGSNLGVFNDNDPYSPKGQNVQDTNQHLCALLMKAGKTWMS
                     YQEDIDLTQNADWQLTNVPLPKDRWTVPLNSFEGAFAPGSTNAYNGTDRYAYAAKHNP
                     MAFFVDTNGDAITTVTNPLVGHYAPLQQLFVDLKNNEVANYNWITPDLYNDMHTSLPG
                     GYKGFTGDQANIRQGDDFLSLVVPMIMASKAYQDGGAIVIWCDETEPDFHGENADDFA
                     HTIPEIIISPLAHPNVNGHPYASQVNMSHSSDLRTWQEIFHVGPYLADAANVQDLSDL
                     FAPGAIPTKP"
     gene            complement(1654..2556)
                     /locus_tag="VN788_07125"
     CDS             complement(1654..2556)
                     /locus_tag="VN788_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4886 domain-containing protein"
                     /protein_id="HXU20335.1"
                     /translation="MMPRGLAFDSWRRIRAIAAALLFTIGAQTAFARTILFVGNSFTF
                     GDLSPVKRFHPERVTDLNKLGFGGVPALFKTFTTEAGLDYDVSLETSPGKSLSWHYAN
                     EQAQLTGKWDVVTLQGYSTLDAKNPGDPADQIKGAHDLAKMLVTENPDAKVYLVSTWS
                     RADQTYKPTGHWYGKSIYQMAEDLAAANAEAVKGFPDLMGVIPVGFAWNRAMKEGVAD
                     SNPFDGVDFGKVDLWTWDQYHGSAEGYYLEALVIFGKITGVDPVSLGARESAANDLGI
                     SPAFAVALQKIAHEELAAEGDSLR"
     gene            complement(2848..3273)
                     /locus_tag="VN788_07130"
     CDS             complement(2848..3273)
                     /locus_tag="VN788_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HXU20336.1"
                     /translation="MTSISALLLQNDAANAALGGGLAAIGGMMLLFWLAIVVVAIVGM
                     WKVFTKAGQPGWAVLVPFYNIYVMTQIAGRPGWWLLLFFIPFVNIAIAIVLAIDIAKA
                     FGQSAVFGVVLLFLLSIVGYLILGFGNYQYRKPVLAAAA"
     gene            3546..>3710
                     /locus_tag="VN788_07135"
     CDS             3546..>3710
                     /locus_tag="VN788_07135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU20337.1"
                     /translation="MIHVENLAKTFDKVEAVKSISFDVAQGEIFAFLGPNGAGKTTTI
                     KMLTTLLRPTS"
BASE COUNT          883 a         1146 c          901 g          780 t
ORIGIN      
        1 aaaactgccg ataagcagcc caaggaaaaa cacatatact ccgatcacga tgggagagag
       61 cacgatttga ctgtgccaca tcgcgctcaa gccctcaagc cgggagccat tcgcacgtgc
      121 ctgatttcta aacgaaatac tcgccaggcg ctgggttttc gcaacgtctc aacggctctg
      181 cgttccagta ttttgctttt tcattcgccc ccactatcca tcattcggct acaatcgtgc
      241 tttatatcaa cgacctgcat ctggggagac cgaaatgtca tactccaaag ttgttcgcac
      301 aattgctgtg tccgccgcgc tgattgccgc aggcgctgcg tctgccgcgg cgcaacaggt
      361 gcagaccgtc ttcgtgatcg cgctcgagaa tcacaattgg acgcagcctg ataacggtcc
      421 cacagcgcgc cttcaccaaa tctataagaa tgccgccgcc cccttcatca acagccttgt
      481 cgacggcacc gccaccgcca ctatcgacgg caaacaggtc gatatcagca agcaggtcgc
      541 ctacgctgct gcctatcaca acgcgcttgc caccgcgaac ggcgacaaca cgcacgtgca
      601 tccttccgag ccgaactaca tctgggccga agccggatcg aacctcggcg tcttcaacga
      661 taacgatccg tactcgccaa aaggccagaa tgtccaggac acaaaccagc atttgtgcgc
      721 gctcttgatg aaggcgggca agacgtggat gtcgtatcag gaagatatcg atctaacgca
      781 aaatgccgac tggcagctta ccaatgtccc tctgccgaaa gaccgctgga ccgtgccgct
      841 caacagtttc gagggcgctt ttgcaccggg atcgacgaac gcttacaacg gtacggatcg
      901 ttacgcttat gccgcaaaac acaatccaat ggcgttcttc gtggatacga acggagacgc
      961 gatcacgacc gtaacaaatc cgctcgtcgg ccactacgcg ccgctacagc aactctttgt
     1021 cgatctcaaa aataacgagg tcgcgaatta caactggatc acgcccgatc tctacaacga
     1081 tatgcacacg agcttgccgg gcggttacaa aggctttaca ggagaccagg ccaatattcg
     1141 ccagggcgac gattttctca gcctggttgt gccgatgatc atggcttcaa aagcgtatca
     1201 agacggcggc gcgatcgtca tctggtgcga cgaaaccgaa cccgactttc acggcgagaa
     1261 cgccgacgat ttcgcgcaca cgattcctga aatcattatc tcgcccctcg cgcatccaaa
     1321 tgtgaacggc catccttatg cgagccaagt caatatgtcg cattcgtcag acctgcgcac
     1381 ctggcaggaa atcttccacg tgggaccgta cctcgctgac gccgccaatg tgcaggatct
     1441 ctccgatctt ttcgcgcccg gcgcgatccc gacgaaacca taggtggcgc agccactctt
     1501 ggcttggtga atttcgatgg caggcggcca ctttcaagtg aatgcggttg attgagtcgc
     1561 ttagaaagtt ggcatcatcc ttcggctcgt ccgcgcaacc ggcgagccga aggacctctc
     1621 tggattttcg ccgccactag ttgtgccgct gtgtcaccgc agcgaatcgc cctctgccgc
     1681 caactcctca tgcgcaatct tctgcaacgc cacggcaaac gcgggcgaaa tccccaaatc
     1741 attcgccgcc gattctctcg cgccgagcga caccggatcg actcccgtaa tcttcccgaa
     1801 aatcactaac gcttcgagat aatagccctc ggcgcttccg tgatattgat cccaagtcca
     1861 caaatcgact tttccgaaat ccacgccgtc aaacggattc gaatccgcca cgccttcctt
     1921 catcgcgcga ttccatgcga atcccacggg aatcacgccc attaggtcgg gaaatccctt
     1981 cacggcctct gcgttcgccg cggccagatc ctcggccatc tgataaatcg atttgccata
     2041 ccaatgtccc gtcggcttgt acgtctgatc cgcgcgcgac cacgtgctca ccaggtagac
     2101 ttttgcgtcc gggttttcgg tgacgagcat cttcgccaaa tcgtgcgcgc ctttgatctg
     2161 atctgccgga tcgcctggat ttttcgcgtc gagcgtgctg tagccttgca gcgtcaccac
     2221 gtcccatttg cccgtcagtt gcgcctgctc attcgcgtag tgccacgaga gcgattttcc
     2281 cggcgaagtc tccagcgaaa cgtcgtaatc gagccccgcc tccgtcgtaa acgtcttgaa
     2341 cagtgcgggc acgcctccaa aaccaagttt gttcagatcg gtcacgcgct ccgggtgaaa
     2401 tctcttcacg ggcgaaagat cgccaaacgt gaaactgttg ccgacgaaca aaatcgtccg
     2461 cgcaaaggcc gtctgcgcgc caatcgtaaa cagcagagcc gcggcaatcg cccgaattct
     2521 gcgccacgaa tcaaaagcta ggccacgagg catcatgcaa actcctccca tctaacgatt
     2581 ttgtcgggga gatataccag acggcgtcca cgaatgcagc ttttattttt gagtctttat
     2641 tttgtaatgc cggcatcttg ccggcgtggt tgcgcactcg cggctgtcag tagcacacag
     2701 aaagaaaatg aggactttgt atctctgagg gatgcagacc aaaataacag aggcgagcgg
     2761 aagcacacac actcttcgcg aatcgacgca aaggacaacc ggtgcttgct tccgctccga
     2821 agcgataacc agtctcgggg tttcttgcta ggccgcagcc gcgagaacag gcttccggta
     2881 ctggtaattc ccgaagccaa ggatcagata gccgacgatg ctcaggagaa agagcagcac
     2941 aactccgaac acggcgctct gcccaaaggc cttcgcgatg tcgatggcaa gcacaatcgc
     3001 gatcgcgata ttgacgaacg gaatgaagaa cagcagcagc caccatcctg gccgccctgc
     3061 gatttgcgtc atcacataaa tgttgtagaa gggaacaagc accgcccatc ctggctgtcc
     3121 ggctttcgta aataccttcc acatgccgac aatagccacc accactatcg caagccagaa
     3181 cagcaacatc attccgccaa tggcggccaa tccaccacca agagccgcgt ttgcagcatc
     3241 gttctgtaga agcagggccg aaatgcttgt catcgggata gcctcccagt agataagatt
     3301 ttggatccca tggcctaaat ttcgcggagt atataccagt cggctccgaa gatatcatcg
     3361 ttctactcct cccgctcgcc gtgtgcgcga aatgccattg ccgcctacgc gcaccagcct
     3421 atgttcttgt gaatgcagca attgtgggtg ctttccaatt ctctgctttt gtctcttgac
     3481 acactaaact aaaccgttca gtattgcagc cacaccaaca cactcaacaa aggggaggag
     3541 tccgcttgat ccacgttgag aaccttgcaa agacgtttga caaagttgaa gccgtaaaga
     3601 gcatctcctt tgacgttgcc cagggcgaga tctttgcctt tctcggccct aatggcgccg
     3661 gcaaaaccac aacgatcaaa atgctcacca cgctgcttcg gccaacgagc
//
LOCUS       DATNDT010000220         7832 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4038468, whole genome shotgun sequence.
ACCESSION   DATNDT010000220 DATNDT010000000
VERSION     DATNDT010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7832
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4038468"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="VN788_07140"
     CDS             <1..661
                     /locus_tag="VN788_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HXU20338.1"
                     /translation="EAGLSATGDIFDAIARRANTTVAALSKGLEKYRAGETGLLRLTW
                     DNGDRTVLVNPELGGVTLGWNLTHTAQDELFAAIEGTAMHTRVIFERMEEHGVPIRRT
                     INAGGIPGRNETLNQVYANILNKPILVPQGDVTSLGSVIFAFMAAGTFKTLEEAQDAL
                     CPKYRVYEPEPAGTAVYQELFALYRKLYFAFGKRKSEAVEIGAVLPELRAIAARVRSS
                     K"
     gene            658..1350
                     /gene="araD"
                     /locus_tag="VN788_07145"
     CDS             658..1350
                     /gene="araD"
                     /locus_tag="VN788_07145"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HXU20339.1"
                     /translation="MSLQSLRDEVYEANLELVRAGLVIDTFGNASGMCRKCGLVAIKP
                     SGVPYDKLTPADLVVSDIEGKIVEGELRPSSDLPTHLALYKAFPGIGGVAHTHSTYAT
                     SWAQACREIPCLGTTHADYWHGAIPVTEPLTPEEIAANYEWNTGLAIAKRFREIDPLE
                     IPSVLVAGHAPFCWGTTPAKAAHTAMILEQAARMAYYTVTLNAGISTLDSVMHDKHFL
                     RKHGPQAYYGQR"
     gene            complement(1364..1666)
                     /locus_tag="VN788_07150"
     CDS             complement(1364..1666)
                     /locus_tag="VN788_07150"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HXU20340.1"
                     /translation="MHHPITSLKTYVLVWAALIVLTGTTWGVAYIDMGPFNIVVAITI
                     AVVKMMLVAVIFMNARHLDHVSKLFAAAGLFWLLILLVFFLSDYLSRDWQPIGKMW"
     gene            complement(1671..2357)
                     /locus_tag="VN788_07155"
     CDS             complement(1671..2357)
                     /locus_tag="VN788_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3 family protein"
                     /protein_id="HXU20341.1"
                     /translation="MADTQTTIVEGHEEIPHLQHHFDDPEQQFNSSQMGMWVFLLTEI
                     MFFGGMFGAYTVYRAMYPGAFASTSHHMDTALGAINTMVLLCSSLTMVLAVRSAQLGR
                     KKPLLSFIGITVFFGLIFLFIKGLEYYHKYVEHLIPGYGFQYPQQAYFHPAQILFCLY
                     FVMTGFHALHMIIGIGLMTWLFIKAKNDTFTPEYYTPVEICGLYWHFVDIVWIFLFPL
                     LYLIGLHLPG"
     gene            complement(2350..3984)
                     /gene="ctaD"
                     /locus_tag="VN788_07160"
     CDS             complement(2350..3984)
                     /gene="ctaD"
                     /locus_tag="VN788_07160"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HXU20342.1"
                     /translation="MSTVAAVHEPRAEERENYLNANYGWKSWLFTTDHKRIAILYIVS
                     ITLFFFIGGFFALLMRLELATPQGDLVSSDTYNKLFTMHGIIMVFFFLVPSIPATLGN
                     FLIPLMVGAKDLAFPRINLLSWYIYIIGGIFAISAIAMGGVDTGWTFYTPFSSTYSTT
                     FVVAAAAGIFIAGFSSILTGLNFIVTIHKMRAPGMTWGRLPLFVWSHYATSLIQILGT
                     PVIAITLALLGLERIFHIGIFDPSIGGDPILFQHLFWFYSHPAVYIMILPGMGVISEI
                     VPCFSRKRIFGYNFVAASSIAIAVLGFLVWGHHLFVTGQSMYASLMFSILSMVVAVPS
                     AIKVFNWTATMWRGSISYQTPMLYAFGFIGLFTIGGLTGVFLATLPIDVHLTDTYFVI
                     AHFHYVMVGGMVMAYLGGMHFWWPKITGKLYPEGWAKFGALIVFVGFNLTFFPQFILG
                     YQGMPRRYHAYAPEFQVLNVMSSAGASILGVGYLIPFIYLLWSLKYGKPAGKNPFGAK
                     GLEWTTYSPPPTFNFDEQPVMREEAYAYGEKEEVVG"
     gene            complement(3981..5099)
                     /gene="coxB"
                     /locus_tag="VN788_07165"
     CDS             complement(3981..5099)
                     /gene="coxB"
                     /locus_tag="VN788_07165"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HXU20343.1"
                     /translation="MGEFQLFPEAASVGATQVDHLYWFLCAVGIVFTALIFLCVFIFA
                     IKYRRKSEDEIPHHVEGSMKLEIVWSVIPFLIMLVMFFWGAKVYFTNYTPPPNALDIY
                     VTGKQWMWKVQYPGGQREINELHVPINRPVKLTLASEDVIHSFYVPAFRLKRDVVPGR
                     FNTEWFTATKPGRYHLFCAEYCGTEHSGMIGWVTVMEPADYQNWLAGGGATGSMAQQG
                     SQLFQQLGCSTCHLQDEQGRCPILKGVYGSRVLLDDGRTVLADDAYVRESILNPNAKI
                     VAGFKPDIMPTFQGQVSEEQLLQLIVYVKSLAVKPNSPMSAAQTGSSEGMHRTGGVNA
                     PSQAAGGNEITTQPARESAFQQIHRRMTKPEGKPEEKK"
     gene            complement(5104..5958)
                     /locus_tag="VN788_07170"
     CDS             complement(5104..5958)
                     /locus_tag="VN788_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HXU20344.1"
                     /translation="MWLSKTALFSGLCLLAFSGALEAQQFGRPTALRGVGIDQNLNGQ
                     LPLNLTFKDESGQDVRLGSYFQRKPVILTLVYYECPMLCNLELNGLISTLKKISLVPG
                     KDYELVAVSFEPKETPKLAAAKKAGYVEKFGNPAAAEGFHFLTGKDPEIHQLAKAVGF
                     HYNWDKVTNQWAHASGIMIATPEGLLSRYFYGVTFQQRDLRLGLVEASNNKIGSPTDQ
                     ILLLCFHYDPTTGKYGLVIMNVLRGVGSAFVILMAFFIYFLIRRQKKKQHGPGELPAA
                     RDSHWRSA"
     gene            complement(5945..6412)
                     /locus_tag="VN788_07175"
     CDS             complement(5945..6412)
                     /locus_tag="VN788_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20345.1"
                     /translation="MESAVTPANHHPHGHHEHKEADVRLVLIATLILIVTIVAVCFIT
                     VGIFDWLNTSPPEAINSAIPVNPNPAPPVPRVLEHPWEELPEVHAHENQVLYSYGWVD
                     KANGVVRVPIDRAMDMLLAKGLPVTPTETTKPAKASAARATVTKAAVKQNVAQ"
     gene            complement(6414..7598)
                     /locus_tag="VN788_07180"
     CDS             complement(6414..7598)
                     /locus_tag="VN788_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20346.1"
                     /translation="MTLAESTPLREKLGRIQNAAFVIGIIVFAACFIELFTDRDVFFR
                     SYLWACLYWAGLAIGCLGVNLMHNVVGGKWGVPIKRILEAGTKTLPWIALLLLPALFF
                     GLHSLYIWSLPSALHDKNIQAKAAYLNPTFFMLRAVGYFILMVLFALRLNRLSDEQDR
                     TGNQETRNRMRMFSAPALLAFTFIVTFAFFDWVMSLEPHWYSTIYGLIFAVGQVLEAL
                     SFCAIVLALLYQTPPFRGVVKSNHFWDLGNMMLCFTMLWAYCGFSQFLIIWSGNLPQE
                     IIWYVRRFSGGWGAIAFIIGVFHFCVPFFLLLMRVMKRDPKWLMRLAIYMLVVRLLDT
                     LWFVLPAFYQRGMVIHWTDFAAVIGIGGIWLGLFLMYFKARPLLPLHDARLPLQAVEE
                     HA"
     gene            complement(7599..>7832)
                     /locus_tag="VN788_07185"
     CDS             complement(7599..>7832)
                     /locus_tag="VN788_07185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20347.1"
                     /translation="TDVPEEQRAKLMAASEKEDIPEPNPPNTGTSYTPPPPEPAAMEG
                     LRGAELPNPYSGEIPPATTQKSAAAAPATGGSK"
BASE COUNT         1944 a         2085 c         2269 g         1534 t
ORIGIN      
        1 cgaggccggt ctgtccgcaa ccggcgatat tttcgatgcg atcgcgcggc gggcgaatac
       61 cacagtcgcg gcgttatcga aaggattgga gaaatatcgc gcgggcgaga cgggattgct
      121 gcggctgacg tgggataacg gtgaccggac ggtgctggtg aatcccgagc tcggcggcgt
      181 aacgctcggg tggaatctca cgcacaccgc gcaggacgag cttttcgcgg caatcgaagg
      241 cacggcgatg cacacgcggg taatcttcga gcggatggaa gaacacggcg ttccgatccg
      301 ccggacgatc aacgcgggtg gcattccggg gcgcaacgaa acgctgaacc aggtttatgc
      361 gaacattctg aacaagccga ttctggtacc gcagggcgat gtgacgagcc ttggatccgt
      421 aatctttgct tttatggcgg ctgggacgtt caagactctc gaagaggcgc aagacgctct
      481 atgcccgaaa tatcgcgtgt atgaaccgga accggcaggc accgcagtat atcaggagtt
      541 gttcgcgctg taccggaagc tctatttcgc gttcggaaag cggaaatcgg aggcggtgga
      601 gatcggcgcg gtgctgccgg aattgcgggc gatcgccgcg cgggtaagga gttcgaaatg
      661 agtctgcaat ctttgcgcga cgaggtctat gaggcgaacc tggagcttgt ccgcgcgggt
      721 ctggtcatcg acacgttcgg caatgcgagc ggcatgtgcc gcaaatgcgg gctggtggcg
      781 atcaagccga gcggagttcc gtacgacaag ctgacgccgg cggatctggt tgtgagcgac
      841 atcgaaggca agattgtaga aggggagttg aggccatcgt ccgacctgcc aacacatctc
      901 gccttatata aagctttccc gggaattggc ggcgtggcac acacccattc gacttatgcg
      961 acttcatggg cgcaggcgtg ccgcgagatt ccttgcctcg gtacaaccca tgccgattac
     1021 tggcatgggg cgataccggt tactgagcca cttactccgg aggagatagc ggcaaactac
     1081 gagtggaaca ccggccttgc aattgccaag cgttttcggg agattgatcc attagaaatt
     1141 ccatcggttc tcgtggcggg acacgcgccg ttttgctggg ggacgacgcc cgcgaaggcg
     1201 gctcacacgg cgatgattct cgaacaggcc gccagaatgg catattacac agtcacgcta
     1261 aacgctggga tctcaaccct ggacagcgtt atgcacgata agcacttcct gcgcaagcac
     1321 gggccacagg cgtactacgg acaaaggtaa cgcggtgggc aatctaccac atcttgccga
     1381 ttggctgcca gtcgcgcgag agatagtcgc tcaagaaaaa tacgagcaga atcagcagcc
     1441 agaataagcc agccgccgca aacaacttac ttacatggtc aagatgtctc gcattcatga
     1501 agatgaccgc gacaagcatc attttgacaa cggcgatcgt gatggccaca acgatattga
     1561 atggccccat gtcgatgtag gcgacgcccc acgtagtgcc agtaagtaca atcaaggcgg
     1621 cccagactag gacgtaagtt ttgagagatg tgatgggatg atgcatggtt ttatccgggt
     1681 aagtgcaatc caatcaagta caacaacggg aacaagaaaa tccagacgat atcgacaaag
     1741 tgccagtaaa ggccgcagat ttcgactggc gtgtaatatt caggggtgaa cgtatcgttc
     1801 ttcgccttaa taaataacca agtcatgagg ccgatgccga taatcatgtg cagggcatgg
     1861 aagccggtca taacgaagta gaggcagaac agtatctggg ccgggtggaa gtatgcttgt
     1921 tgggggtact gaaagccata accgggaatc aagtgctcca catacttgtg ataatattcc
     1981 aggcccttga taaacagaaa gattaagccg aagaaaaccg taatgccgat gaacgaaagc
     2041 agcggcttct tccggcccaa ctgcgccgac cggaccgcga gcaccatagt aagactgctg
     2101 caaagcagca ccattgtgtt aatcgcgccg agcgcggtat ccatgtggtg gcttgtgctg
     2161 gcgaacgcgc ctggatacat ggcgcggtag accgtatagg cgccaaacat gccaccgaag
     2221 aacatgattt cggtaagcag aaagacccac atccccatct gggaggagtt gaactgctgc
     2281 tccgggtcgt caaagtggtg ttgaaggtgc ggtatttcct cgtggccttc cacgatggtg
     2341 gtttgcgtat cagccaacga cttcctcctt ctcaccgtac gcgtaggcct cttccctcat
     2401 gacgggctgc tcgtcgaaat taaaagtggg cggcggagag taagtggtcc actcgaggcc
     2461 cttagcgccg aatgggttct taccggcggg cttaccgtac ttcaaagacc acagaagata
     2521 gataaatgga atcaaatagc caacaccgag gatcgacgcg ccggcgctcg acatcacgtt
     2581 cagaacctgg aactcgggcg cataggcgtg atagcggcgc ggcattccct ggtagcccag
     2641 aataaactgg ggaaagaaag tcaggttgaa tccgacgaac acaataagcg ctccaaattt
     2701 cgcccaacct tcgggataca acttaccggt tatcttcggc caccagaaat gcataccgcc
     2761 cagataagcc atgaccatgc caccgaccat cacatagtgg aaatgcgcga tgacaaagta
     2821 agtgtcggtg agatgaacgt cgatggggag cgtcgcgaga aaaacgcccg tcaacccgcc
     2881 gatggtaaac agaccgataa aaccgaacgc gtataacatc ggcgtctgat aagagataga
     2941 cccgcgccac atcgtggctg tccaattgaa gactttaatc gccgacggaa ccgcgacaac
     3001 catactcagg atggaaaaca tgaggctggc gtacatcgac tgaccggtca cgaagagatg
     3061 atgtccccaa acgaggaagc ccaaaaccgc gatggcgatg ctcgatgcag cgacgaaatt
     3121 gtagccaaag atgcgcttcc gggagaagca ggggacgatc tcactgatca ctcccatgcc
     3181 gggcagaatc ataatgtaaa cggccggatg cgagtagaac cagaaaagat gctgaaacag
     3241 aatcgggtcg ccgccgatgc tggggtcgaa gattccaatg tggaagatcc gctccagacc
     3301 gagaagggca agggtgatcg caatcacagg cgtgccaagg atctggatga ggctagtcgc
     3361 gtagtgggac cagacgaaca gtggcagtcg gccccacgtc attcccggcg ctctcatctt
     3421 gtggatggtg acaatgaagt tcaagccggt gagaatcgag gaaaaaccag caataaagat
     3481 gcccgccgcc gccgcgacaa cgaaggtagt ggagtaagtg ctgctgaaag gggtgtagaa
     3541 ggtccagccg gtatccacgc cacccatcgc gatggcgctg atcgcgaaga tgccgccgat
     3601 tatatagatg taccagctca gcaagttaat acgcgggaag gcgagatctt tggcgccgac
     3661 catgagaggg atcaggaagt tgccgagcgt cgccggaatc gacggtacca gaaaaaagaa
     3721 caccatgata atcccatgca tggtgaacaa cttgttgtag gtatcggaac tgaccagatc
     3781 gccttggggc gtcgccagtt ccaggcgcat gagcaaggcg aagaagccgc caatgaagaa
     3841 gaacagggtg atcgagacga tatacaggat cgcgatccgt ttgtggtccg tggtgaaaag
     3901 ccacgatttc cacccgtaat tcgcattgag ataattctcg cgctcttccg cgcggggctc
     3961 gtgaactgct gctacggtgc tcatttcttt tcctcaggct ttccctcagg ctttgtcatt
     4021 ctccggtgga tctgctggaa ggcggactcc ctggccggtt gcgttgtgat ttcattgccg
     4081 cccgccgcct gtgaaggagc gttcaccccg ccggtacgat gcatgccctc cgaggaccca
     4141 gtctgcgcgg cgctcatagg tgagtttggc ttcaccgcaa gggacttcac gtagacgatc
     4201 agttgaagca gttgttcttc gctgacttgc ccctgaaacg taggcataat atccggcttg
     4261 aatccggcga cgattttcgc atttggattc aggatcgact cccgtacata ggcatcgtcc
     4321 gcaaggaccg tgcggccatc gtcgaggaga acccgcgatc catacactcc cttcaggata
     4381 ggacaacggc cctgctcgtc ttgaaggtgg caggtgctac agccaagctg ctgaaataac
     4441 tgcgaaccct gctgagccat cgagccggtc gctccaccgc cggctagcca gttctgataa
     4501 tcggcgggct ccatgacagt aacccagccg atcatgccgg aatgctccgt gccgcagtac
     4561 tcggcgcaaa acaggtgata gcggcccggc ttggtcgcgg taaaccactc ggtgttgaag
     4621 cggcccggaa ccacgtcgcg tttgaggcga aacgccggga cgtaaaagct atgaataaca
     4681 tcctcggagg ccagagtcag cttgaccggg cgattgatcg gcacgtgcag ttcattgatt
     4741 tcacgctgcc cgcccggata ctgaactttc cacatccact gcttgccggt gacatagata
     4801 tcgagagcat tgggaggtgg cgtgtaattg gtgaaataaa ccttagcgcc ccagaaaaac
     4861 atgaccagca tgattaggaa cggaattacg gaccacacga tctcgagctt catggatccc
     4921 tcgacatggt gcgggatctc gtcttcggat ttccggcgat acttgatggc gaaaataaaa
     4981 acgcacagga agatcagcgc cgtgaatacg ataccgaccg cgcacaggaa ccaatagaga
     5041 tgatctacct gagtggcgcc aacggaagct gcttccggga aaagctggaa ttcacccatt
     5101 cagttaagcg gacctccaat gactgtctcg cgccgccggc aattcgccgg gtccgtgttg
     5161 ttttttcttc tggcggcgga tcaggaagta aataaaaaaa gccatgagta ttacaaacgc
     5221 gctgcccaca ccccgaagca cattcatgat caccagaccg tatttccctg tcgtgggatc
     5281 gtaatggaag caaagcagca gaatctgatc ggtcgggctt ccgatcttgt tattcgaagc
     5341 ctcgacaagg ccgaggcgca ggtcccgctg ctgaaacgtg acgccataaa agtaccgcga
     5401 gaggagccct tcgggagtgg cgatcataat gccgctcgcg tgcgcccatt gattcgtaac
     5461 cttatcccag ttgtagtgaa aaccgaccgc cttggcgagt tgatgaatct ccggatcttt
     5521 tccggtgagg aagtgaaatc cctcggcggc ggccggattg ccaaattttt cgacgtaacc
     5581 cgccttcttc gccgcggcga gttttggcgt ctctttcggt tcgaagctga ccgccacgag
     5641 ctcgtagtcc ttacccggca caagagagat cttcttcaac gtactgataa gcccattcaa
     5701 ttcgagattg cacagcatcg ggcactcgta atatacgagc gtcaggatta ccggcttccg
     5761 ctgaaaataa ctgcccagac gcacatcttg tccggactca tccttaaaag tcagattcag
     5821 gggaagctgg ccgttcaagt tctgatcgat gccgactcca cgcaatgcgg tgggccgccc
     5881 aaactgctga gcttccaacg caccgctaaa ggcgagtagg caaagaccgg aaaatagagc
     5941 cgtcttactg agccacattc tgtttcactg ctgctttcgt aaccgtggcc cgggccgctg
     6001 aagccttggc cggtttggtt gtttcagttg gcgtgactgg aagtcctttt gcaaggagca
     6061 tatccatcgc ccggtcgatg gggacacgaa ccacgccatt ggccttgtcc acccagccgt
     6121 aagaatagag aacctgattc tcatgggcat ggacttcagg cagttcttcc cagggatgct
     6181 ccaatacgcg cggcacggga ggagcgggat tgggattaac gggaattgcg gaattgatcg
     6241 cttccggcgg gctggtatta agccagtcga aaatgccgac ggtgatgaaa catacggcca
     6301 cgatggtgac aatcagaatc aacgtcgcga tcaagaccag acgaacatcg gcctctttat
     6361 gctcgtgatg gccgtgcgga tgatgattgg ctggcgtgac agcgctttcc atatcaggcg
     6421 tgctcctcca ccgcctgcaa aggcaggcgc gcatcgtgca acggcaatag cggacgggcc
     6481 ttgaagtaca tcagaaacag ccccagccag attccgccaa ttccgatcac ggccgcgaaa
     6541 tcggtccagt ggatcaccat gccgcgctgg tagaaggcgg gcaaaacaaa ccagagcgta
     6601 tcgagcaagc gcacaaccag catataaatg gcgagccgca tgagccattt cggatccctc
     6661 ttcatgactc gcatgagcag gagaaaaaat ggaacgcaga aatggaaaac gccaatgatg
     6721 aaggcgatgg ctccccagcc tccgctgaag cggcgaacgt accagataat ctcctgggga
     6781 agattgcccg accagataat gaggaactgg gagaatccgc agtaggccca cagcatcgtg
     6841 aagcagagca tcatgttgcc gagatcccag aaatggttgc tcttcaccac gccccggaaa
     6901 ggcggcgtct ggtagagcag cgcgagaacg atagcgcaaa aagaaagcgc ctccaggacc
     6961 tgtccgacgg cgaagatgag tccgtagatc gtcgaatacc aatgcggctc aagcgacatc
     7021 acccaatcga agaacgcgaa agtcacgatg aaggtaaagg ccagcagcgc gggcgcgctg
     7081 aacatgcgca tgcggttccg ggtctcctga ttcccggtgc ggtcctgttc gtccgaaaga
     7141 cgatttaagc gcaacgcgaa aaggaccatg aggatgaagt acccgaccgc ccggagcatg
     7201 aagaacgttg ggttcagata cgccgctttg gcctgaatgt tcttgtcgtg cagtgccgac
     7261 ggaagcgacc agatgtagag tgaatgcaat ccgaaaaaca atgcgggaag caacagcaat
     7321 gcaatccagg gcagcgtctt tgtgccagct tccaagatcc gcttgatcgg gacgccccat
     7381 ttcccgccca ccacattgtg catgaggttg acgccgagac agccgatcgc caagcctgcc
     7441 cagtacaggc atgcccagag gtacgagcgg aagaagacat cacggtcggt gaaaagctcg
     7501 ataaagcagg ccgcgaagac aataatgccg atgacgaatg cggcattctg gattcgccct
     7561 aacttttccc gaagaggagt gctttcagct aaagtcattt atttcgatcc tccggtcgcc
     7621 ggggcagcag cggcggattt ctgagtggtc gcgggtggga tctcgcccga gtatggattc
     7681 ggaagttccg ccccgcggag tccttccatc gccgcgggtt ccggcggcgg aggcgtataa
     7741 ctggtaccgg tattcggagg attgggctcg gggatatctt ctttttctga agcggccatc
     7801 agtttggcgc gctgctcttc gggcacgtcg gt
//
LOCUS       DATNDT010000221         3180 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_660804, whole genome shotgun sequence.
ACCESSION   DATNDT010000221 DATNDT010000000
VERSION     DATNDT010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660804"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="pnp"
                     /locus_tag="VN788_07190"
     CDS             <1..1011
                     /gene="pnp"
                     /locus_tag="VN788_07190"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HXU20348.1"
                     /translation="EAFKRFMLHYNFPPFSVGEVKFLRGPGRREIGHGALAERALANL
                     LPSEADFPYAMRVVSDILESNGSSSMATVCGGSLALMDAGVPLKSPCAGIAMGLVVEG
                     DKYAILTDIAGAEDHYGDMDFKVAGTRQGITALQMDIKVAGVSIAMMREALEQARKAR
                     LQILDTMESTISSGRAEISAYAPHLHILSIPTDKIRDLIGPGGKKIRSIIEATGVKID
                     VMDDGKVHVFASNGPAAESALKMIRDTTATAEIGKTYLGTVVRIAEFGAFVEIFPGTD
                     GLLHISEIAEARIRAVRDELKEGDQVLVKVLAIEGNKIRLSRKAVLRDQRDKQQKAET
                     AG"
     gene            1098..1541
                     /locus_tag="VN788_07195"
     CDS             1098..1541
                     /locus_tag="VN788_07195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HXU20349.1"
                     /translation="MPVCTAAASTDESAIRDLVGARQQQAWNQHDARAYAALFAEDSD
                     VVNVMAWWWKGHGEIESNLTLMFANIFRESTLTFGQVDVRFLTPDIAIAHAHWTMTGA
                     HMPPGLPEPREGLQTLTVQKRNGRWLIALSRTPTTCPGQQAHNEK"
     gene            complement(1572..2843)
                     /gene="murA"
                     /locus_tag="VN788_07200"
     CDS             complement(1572..2843)
                     /gene="murA"
                     /locus_tag="VN788_07200"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HXU20350.1"
                     /translation="MDRFKIAGGRRLEGAVQISGAKNAALPAMAATLLTAEPVRLQNI
                     PHVRDIITMAKLLAHMRCRVESPDIPPSEFTIQAETVSHTEAPYELVKTMRASVLSLG
                     PLVARFGYARVSLPGGCSIGARPIDIHIQALEKLGATISVEHGYVEARANRLRGAGFR
                     FPKITVTGTENVLTAAVLAEGETLLENCALEPEVTDLAALLVKMGAQIEGAGTSTIHV
                     HGVEKLHGATHAVIPDRIEAGTFLVAGAITGGELLLTCCQPRHLTAIINKLEECGVEI
                     HCEGESALRVRAAKRLLAADVTTEEYPGFATDMQAQFMALATQAHGVSRIRETIFENR
                     YLHASEMMRMGANIAIEGNLATVTGPTPLSGAPVTASDLRASASLVLAALVADNTTWI
                     DRVYHIDRGYERIELKLRELGARIERVSTGD"
     gene            complement(2905..>3180)
                     /locus_tag="VN788_07205"
     CDS             complement(2905..>3180)
                     /locus_tag="VN788_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20351.1"
                     /translation="RVYDNWMVHVALRNQKLQWRGSQILEEAAGVSALTGKRALRKAK
                     YDLPAALVMLKTGADLRAARQSLAIAHGNVREALEMAKKRSRKQRSE"
BASE COUNT          626 a          909 c         1005 g          640 t
ORIGIN      
        1 gaagcgttca agcggttcat gctgcactac aattttccgc cgttctccgt aggcgaagtg
       61 aaatttctgc ggggacccgg ccggcgcgaa atcggccacg gagcattggc ggagcgcgcg
      121 ctagcgaacc tgcttccttc ggaggcggat tttccttacg cgatgcgcgt ggtttccgac
      181 attctggaat cgaacggatc ttcgtccatg gcgacggtgt gcggcggatc gctggcgttg
      241 atggacgcgg gcgtaccgtt gaaatctcca tgcgccggaa tcgcgatggg cttggtggtc
      301 gagggcgata agtacgcgat cctcacggat atcgcgggcg cggaggacca ctacggcgat
      361 atggatttca aagtggcggg tacgcggcaa gggatcacgg cgctgcagat ggatatcaaa
      421 gtggccggcg tgagcatcgc catgatgcgc gaggcgctgg agcaagcgcg aaaggcgcgg
      481 ttgcaaattc tcgacaccat ggaatcgaca atttccagcg ggcgcgcgga aatttccgcg
      541 tacgcgccgc atctgcatat cctcagcata ccaaccgaca agattcgcga tctgatcgga
      601 cccggcggca agaagatccg gtcgatcatc gaggccacgg gcgtgaaaat cgacgtgatg
      661 gacgacggca aagtgcacgt gtttgccagc aatggccccg ccgccgaatc cgcgctgaaa
      721 atgattcgcg ataccactgc cacggcggag atcggaaaaa cgtatctcgg taccgtagtg
      781 cgcatcgcgg aatttggcgc gtttgtcgaa atttttcccg gcaccgacgg cttgctgcac
      841 atcagcgaaa tcgcggaagc gcgcatccgc gcagttcgcg acgagctgaa ggaaggcgat
      901 caggtgctgg taaaagtgct ggcaatcgag ggaaataaaa tccgcttgag ccgcaaggca
      961 gtgttgcgcg accagcggga caagcaacag aaggccgaaa cggcgggctg atcggcatct
     1021 ggaaccatgg cacagccgag gtcacgaatg gtctggcgat ttctgacgct tgcgattctt
     1081 tttcgacttg ccttccgatg ccggtgtgca ccgcggccgc ttcgaccgac gaatcggcca
     1141 ttcgcgactt agtcggagcg cgccagcagc aggcgtggaa tcaacacgat gcacgcgctt
     1201 acgcggcgct gttcgcggaa gacagcgacg tcgtcaatgt gatggcatgg tggtggaagg
     1261 gccacggcga gatcgaaagc aacctcacgc tcatgttcgc caacattttt cgcgagagca
     1321 ccctcacctt tgggcaagtg gacgtacgct ttttgacgcc ggacatagcc attgcgcacg
     1381 cccactggac gatgaccggg gcgcatatgc cgccgggact gcccgagccg cgcgaaggct
     1441 tgcagactct gacggtacag aaacgcaatg gaaggtggct gatcgcactt tccagaacac
     1501 caactacgtg ccccggccag caagcccaca atgagaagta ggtggtgcgg taatagcttt
     1561 cgttgctgcg ctcaatctcc ggtgctaacg cgctcgatgc gggcgccgag ttcgcgcaac
     1621 ttcagttcga ttcgctcgta gccgcggtcg atgtgataga cgcggtcaat ccaggttgtg
     1681 ttatccgcca ccagagcggc aagcacgagg ctggcgctgg ctcgcagatc cgatgccgta
     1741 accggcgcgc cgcttagggg agtcgggccg gtgacggtcg cgaggtttcc ttcgatggcg
     1801 atgtttgcgc ccatgcgcat catttcgctg gcatgcaaat agcgattttc gaagatcgtc
     1861 tcgcgaatgc gcgagacgcc gtgggcctga gttgcaaggg ccatgaactg ggcctgcata
     1921 tcggtggcga aaccgggata ttcctcggtg gttacgtcag cggctagcaa cctttttgcc
     1981 gctcgaacgc gcagcgcgct ttcaccctcg cagtgaatct cgactccgca ttcctcaagc
     2041 ttattgatga tggcggtgag gtgcctgggc tggcagcagg tgagcagcag ttcgccgcca
     2101 gtgatggctc ccgcgaccag gaacgtaccg gcctcgatgc gatcggggat tacggcgtga
     2161 gtggcgccat gcaatttttc cacgccatga acgtgaatgg tcgaggttcc cgcgccctcg
     2221 atctgcgcgc ccattttcac gagcagcgcg gcaaggtcgg tgacctcggg ttcgagggcg
     2281 caattttcga gcagggtttc accctccgcg agaaccgcgg cagtgaggac gttctccgtt
     2341 cctgtgactg tgatttttgg aaagcgaaac ccagcgccgc gcaggcgatt cgcgcgcgct
     2401 tccacatagc cgtgctccac cgaaattgtg gctcccaact tttcgagggc ctgaatgtga
     2461 atgtcgatgg ggcgcgcgcc gatggagcaa cctccgggca gtgaaacgcg cgcgtaaccg
     2521 aaacgcgcca cgagcggtcc gagcgacagc accgaggcac gcatggtctt cactaattcg
     2581 tacggcgctt cggtgtgcga tacggtttcg gcttgaatgg tgaattcgct gggcggaata
     2641 tccgggcttt cgacgcggca gcgcatgtgg gccagcaatt tggccatggt aatgatgtcg
     2701 cgcacgtgag gaatgttttg caggcgcacc ggttcggccg tgagcagcgt ggctgccatc
     2761 gctgggagcg cggcgttttt tgcgccactg atttggacgg cgccttcgag acgtctgccc
     2821 cccgcgattt tgaatcgatc catctctagc ccttcttttc ctcacttcac gaaaaaccca
     2881 cgacgagaac ggcgctcgtc gtggctactc agagcgctgc ttgcgtgagc gctttttcgc
     2941 catttctaat gcttcgcgca cgttcccgtg ggcaatggcg agcgattgcc gggcggcgcg
     3001 caaatcagcg ccggtcttca gcattaccag agccgcgggc aaatcgtatt tcgccttccg
     3061 cagggcgcgt tttcctgtga gcgcgctgac tcccgcggct tcctcgagga tttgagagcc
     3121 acgccactgg agcttttgat tgcgaagcgc aacgtggacc atccagttgt catacacgcg
//
LOCUS       DATNDT010000222         2506 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2045023, whole genome shotgun sequence.
ACCESSION   DATNDT010000222 DATNDT010000000
VERSION     DATNDT010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2506
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2045023"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2506
                     /locus_tag="VN788_07210"
     CDS             <1..>2506
                     /locus_tag="VN788_07210"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HXU20352.1"
                     /translation="MMVTCTYDHRVIQGAESGMFLGRLQALLEGEDGFYDRIFHDLGT
                     PFKPFRWEAERKGMAPGFSGSNREEAAKQAAVFQLIHAYRVRGHLMADLDPLGQERRE
                     QHPELDPAIYGLSMWDLDREFLAGTLRVNGHGIRAAAPLREILETLRQTYCGKLTCEY
                     MHIQHPDEKGWLQERMEPSANRWPLDKTVRIRALERVIEAEEFEHFLHTRFIGHKRFS
                     LEGAESAIVILDEVLERAARASVHEVVIGMAHRGRLNLLANIVGKSMVQVFSEFEGNV
                     DPESTQGSGDVKYHLGASGVRRSSEGKDIVVSVAFNPSHLEAVDPVVEGIVRPKQDRL
                     GDVNRERVIPLLIHGDAAFAGQGVVAETLNLSQLEGYRTGGTIHLVINNQIGFTTNPE
                     AARSSAYSTDIARMVQAPILHVNGDDPESCMRAAQLAYDYRQQFKKDVVIDMVCYRRH
                     GHNEADDPSYTQPLLYRKIRNHPSVAVIYSERLVREKLADAPGVEQMRKAVSTRLNNI
                     YDESKRNSERYEVQELSAVGVEPSEAPHASTAIERTVLERVITGATSFPEQFHLHPKL
                     EGFIKKRREALHGTPIDWAFGEALAFGSLVLEGTPVRLSGQDSGRGTFSQRHLEFYDS
                     ENGELYVPLSHLSPDQARFDVIDSSLSEYAVMGFEFGYSVADPLTLVLWEAQFGDFAN
                     GAQIMIDQFIASAETKWGQPSGLVLLLPHGYEGQGPEHSSARIERFLQLAAEGNIQVA
                     NCSTPAQYFHLLRRQMNGGPDRRGIRKPLIVFTPKRMLRHPRSISTLEDFTSGAFQEV
                     VPDAAKLDSSGVRRILICSGQVYYDLVAAREERKIDN"
BASE COUNT          515 a          700 c          798 g          493 t
ORIGIN      
        1 gatgatggtc acctgcactt acgatcatcg cgtgatccag ggcgcggaat cggggatgtt
       61 cctcggccgc ctgcaagcgc tgctcgaagg cgaagacgga ttttacgatc gcatctttca
      121 cgatcttggg acgcctttca aacccttccg ttgggaagcg gagcggaaag gaatggcgcc
      181 tggatttagc ggcagcaatc gcgaggaagc cgcgaaacaa gcggccgtct tccaactgat
      241 ccatgcctat cgcgtgcgcg ggcatctgat ggcggatctc gatccgctcg gccaggagcg
      301 ccgcgagcaa caccccgagc tcgatccggc gatctacggg ctgagcatgt gggatctcga
      361 tcgcgagttt ctcgcgggga cattgcgggt gaacggacac ggcattcgcg cggccgcgcc
      421 gcttcgcgaa attctcgaaa cgctgcgcca gacctactgc ggcaagctga cttgcgagta
      481 tatgcacatt cagcatcccg atgaaaaggg gtggctgcag gaacgcatgg agccgtcggc
      541 gaatcgctgg cctctcgata aaacggtgcg catccgggcg ttggagcgcg tcatcgaggc
      601 cgaggagttc gagcatttcc tgcacacgcg attcatcggc cacaaacggt tttcactcga
      661 aggcgccgaa tcggccattg tcattctgga cgaagttctg gagcgcgcgg cgagggcgag
      721 cgtgcacgaa gtggtgatcg gcatggcgca tcgggggcgc ttgaatctgc tcgcgaatat
      781 cgttggtaag tcgatggtgc aggttttttc ggagttcgag ggcaatgtcg atcccgaaag
      841 cacgcaggga tcgggcgacg tgaaatatca tcttggcgcg agcggcgtgc ggcgttcgag
      901 cgagggcaaa gacattgtgg tctcggtcgc gttcaatccc agccatctcg aagccgtcga
      961 tccggtggtg gaaggtatcg ttcgtcccaa acaggaccgc ctgggcgacg tcaatcggga
     1021 acgcgtgatc ccgctgctga ttcatgggga cgcggcgttc gccggccagg gcgtagtagc
     1081 ggagacgctc aatctctcgc aactggaagg ctatagaacc ggcggcacga ttcacctggt
     1141 catcaataac cagatcgggt ttacgaccaa tccggaggcc gcgcggtcgt ccgcctattc
     1201 gaccgatatt gcgcgcatgg tgcaggctcc gattttgcat gtgaatgggg acgatccgga
     1261 gtcgtgcatg cgcgccgcgc aactcgccta cgattaccgg caacagttca agaaagacgt
     1321 cgtcatcgac atggtgtgct atcgccgcca cggccacaat gaggcagacg atccgagtta
     1381 cacgcagccg ctgctttacc gcaagattcg caatcatcca tcggtcgctg tgatctattc
     1441 ggagcgcctg gtgcgcgaga aacttgccga tgcgccaggc gtggagcaga tgcgcaaggc
     1501 ggtttccact cgcctaaaca acatttacga cgaatcgaaa cgcaactcgg aacgttacga
     1561 agtgcaggaa ctgagcgcag tgggcgtgga gccatcggaa gctccgcacg ccagtaccgc
     1621 gattgagcgg actgtgctcg agcgcgtaat taccggggcc acgagcttcc ccgaacagtt
     1681 ccatctacac cccaagctgg agggtttcat caaaaagcgg cgggaagcgc ttcacggaac
     1741 tccgattgat tgggcttttg gcgaggcgct cgcattcggg agcctcgtgc tcgaaggaac
     1801 tccggtccgt ctgagcgggc aggatagcgg ccgcggaaca ttcagccagc gccatctcga
     1861 attttacgac agcgaaaacg gcgagcttta cgtgccgttg agccatcttt cgccggacca
     1921 ggcccgcttc gacgtgatcg acagttcgct gagcgagtac gcggtgatgg gcttcgaatt
     1981 cggctacagc gtggccgatc cgctgactct ggtgctgtgg gaagcgcagt tcggcgattt
     2041 cgcgaacggg gcgcagatca tgatcgacca gttcatcgcg tcggctgaga ccaaatgggg
     2101 ccaaccgagc ggattggtgc tgcttttgcc gcatgggtac gaagggcaag gaccggagca
     2161 ttcgagcgcg cgcatcgagc ggtttttgca gttagccgcc gagggaaata ttcaggtggc
     2221 gaattgttct actcccgcgc agtattttca cttactccgg cgccagatga acggcggtcc
     2281 ggaccggcgc ggcatccgta agccgctgat cgttttcacg ccgaagcgga tgctgcgtca
     2341 tccgagatcc atttccactc tggaagattt caccagcggc gcgtttcaag aggtggttcc
     2401 cgatgccgcg aaactggata gcagcggcgt gaggcggatt ctcatctgct cgggtcaggt
     2461 ttactacgat ctagtggcgg ctcgcgaaga gcgcaagatc gataac
//
LOCUS       DATNDT010000223         7975 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_18183, whole genome shotgun sequence.
ACCESSION   DATNDT010000223 DATNDT010000000
VERSION     DATNDT010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7975)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7975)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7975
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18183"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..850
                     /locus_tag="VN788_07215"
     CDS             191..850
                     /locus_tag="VN788_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20353.1"
                     /translation="MVGRYPFFYAYSGSVLLMSILLLSPSLHRHWYWGAQFFTLTLGC
                     GILLEILNHALSPYPGAEKFARMAGLTAFGLVTCFALLLPLAESHWSAADTMHELERD
                     FRMVQAIFIAGLLAVISYYRIALGKNMKGMIGGYGVYVGTSLVGLALGSYSANLFGEA
                     SRMFRPFSFDISMIIWIVALWSYSPDPVPEPGIYLEEDYEAFVAKTRSAMRSYFRKIV
                     R"
     gene            1732..2199
                     /locus_tag="VN788_07220"
     CDS             1732..2199
                     /locus_tag="VN788_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20354.1"
                     /translation="MSSLPRRLDVQEAADDVRQRTLSELPQSLDRLIYLASMRDYNTG
                     LYYHDGLAARFSQEVACEALADCHREVFRQLVACPLENVVGQMEAYMKSTHTSPSEFL
                     AAWRKLEPYRVVVPVEADPLSTQLLFSTFKIALAILEARLTHSPEESGASPRR"
     gene            complement(2123..2986)
                     /locus_tag="VN788_07225"
     CDS             complement(2123..2986)
                     /locus_tag="VN788_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU20355.1"
                     /translation="MAFQSPGSTFHCEAFDVRTRMRPGEQLRELRNRLGVTTREVEEF
                     SRSIAEDRQNEEFYISNAWLTQLENKNSVPSIYKLYSLSVIYRTKFNDLLLVFGIDLS
                     ATSRYQLALPLQNTHLASFEAPSPEKAITFPVRFDKSFNLETTSLIARMVEVWGEVPI
                     ALIQKLDVRHCQYGYIGVQDYTMYPLLRPGSFVQIDNQPTHMQAAEWRTEFDRPIYFV
                     ELRDGYACSWCEVRGSQLTLIPHPLSGCALRQFVYPSEAEIVGQVTGVAMRLIPPESE
                     SDELPKLPRRS"
     gene            3468..3824
                     /locus_tag="VN788_07230"
     CDS             3468..3824
                     /locus_tag="VN788_07230"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU20356.1"
                     /translation="MSSNSAGPSTGSQASGSERRTVPRYSLIATAEVIEPVSEVRISG
                     RISEISRKGVYVDILNPLPRGTRVTIRISRDQGTFVATGKVIYVQEGMGMGVAFLDVA
                     EDQKNLLISWLAELTA"
     gene            complement(3828..4178)
                     /locus_tag="VN788_07235"
     CDS             complement(3828..4178)
                     /locus_tag="VN788_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20357.1"
                     /translation="MTTIGERRSARLLLDVPLMIWGESSEQQAFREETFTVTVSAHGA
                     LVMLAAKVVLGQRVVLRKLTDTVEIRGKIAYAGMPYAGLTQVGIELDRPVPEFWPVHE
                     PPPDWMVSEGQSPA"
     gene            4407..4964
                     /locus_tag="VN788_07240"
     CDS             4407..4964
                     /locus_tag="VN788_07240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HXU20358.1"
                     /translation="MKINRKPSRLATGRMHMAVDRRRRIVAWMRAHGAPVRGGELAKR
                     LRVSRQCLVQDVAILRASGQEILATPQGYRLPKGSSRSHRALLACKHVPERTEEELQI
                     LVDNGVKVLDVIVEHPLYAELRGSLMIESRADVQDFLDRVRSSHASLLSSLTRGVHLH
                     TVEASRPEMIARAKTALRRRGFLLK"
     gene            4981..5940
                     /gene="nadC"
                     /locus_tag="VN788_07245"
     CDS             4981..5940
                     /gene="nadC"
                     /locus_tag="VN788_07245"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HXU20359.1"
                     /translation="MNETRRQRIEKALCRGASLSIDNPQYCQAVRTFSDFLFQADLGT
                     RDLTAEAVGKRSLGARATVLAREGGIAAGLEEFALLLRSHGVRVEFEKKDGDVIRPGE
                     ALLYAEGDEINLLSLERVGLNLVQRMSGIATAARCLQQRVNQRGFTALVVGTRKTHWG
                     LLDKRALHLGSGGTHRLGLGDAILIKNNHLALLAGTEAEAAPLAIEKAWTFRGEAAFI
                     EVEVRGEDAARAAAQTFRRLRADTAHDYPCLLMLDNMAPGEIAAILDALRRENLWSDV
                     LIEASGGISEANLEAYAASGVDAISVGALTHSVRALDVCQRIL"
     gene            5968..6993
                     /gene="nadA"
                     /locus_tag="VN788_07250"
     CDS             5968..6993
                     /gene="nadA"
                     /locus_tag="VN788_07250"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010306643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HXU20360.1"
                     /translation="MASAPTQEFFEARYTLPVVEQIDDTADEIRAKQERVRALCVERN
                     AVLLAHHYQRPEVQEVADAVADSLKLSQAAAKTNADVIVFCGVHFMAETAAILLPDKT
                     VLLPDLRAGCSLAASITPEELLKWKAKLPDAVVVSYINTSAAVKAESDYCCTSANAAK
                     VVRSIPSDKTILFLPDKFLADVVARQTGRSNIVAYPGYCHVHKTIRPEQVVEILEEHP
                     DIELLLHPECGCVSSCMAKAVDGTLPQDRTFFLSTEGMLKHIGESDATEFAVGTELGI
                     FHQLNKKFPQKTFHAVNPNAVCAFMKTITLDRVIQSLETMTPQIRVPEDIARKAKRAI
                     DRMLALA"
     gene            7004..7804
                     /locus_tag="VN788_07255"
     CDS             7004..7804
                     /locus_tag="VN788_07255"
                     /EC_number="1.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014585588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HXU20361.1"
                     /translation="MLKIGLLGVGAIGKTIATALDQKQVDAELVALSDQDCARAEQLS
                     SELSNQPPVLSVEEMIDRCELAVEAASQAALPDFVPKALARGRDMLIMSVGGLLGRHQ
                     WFREAETKGCRIYVPSGAIAGLDGIKSASIGRIDSAELTSRKPVKALAGSKYILDHGI
                     QLDGMKEDTVIFDGSAEEAAQAFPATSNVAASLRLAVDRAAVRVRVVAVPGGSENVHE
                     IRVQGEFGRLSMRVENVPSKTNPRTSQLAAFSAIATLKNLTRSLRVGT"
BASE COUNT         1640 a         2287 c         2255 g         1793 t
ORIGIN      
        1 tgggatgggc ccgtgcccga gccaaagtaa ttccctttcg gttttggcct ggcgaaagag
       61 gagagttgcg gctctcctct tttttgtgtt accgtccagt tcctcacctg taccaatggt
      121 ttcctcggca atctggttca cgggcatcgt gctggaatcg cttcttctta tccggagctt
      181 ttggacccgt ttggttggca gatacccctt cttttatgcc tacagcggat cggtgctgct
      241 catgtcgatc ctgctcttgt ccccaagtct gcatcgtcat tggtactggg gcgcgcagtt
      301 cttcacgcta actctgggct gcggcatcct ccttgaaatt ctaaatcacg ctctgtcgcc
      361 atatcccggt gcggaaaagt tcgcgagaat ggccggtcta acggccttcg ggcttgtaac
      421 ctgctttgcg ctcctcttgc cactggctga gtcccactgg tccgcagcgg acaccatgca
      481 cgaactggaa cgagatttca gaatggttca ggcgatcttc atcgccgggc tgttggcggt
      541 catctcctat tacagaatcg cactgggcaa aaatatgaaa ggcatgatag gcggatatgg
      601 agtatatgtg ggaacgagcc tcgtcggcct cgcgttgggg tcctactcgg cgaatttgtt
      661 tggcgaagcc tcgaggatgt ttcgcccctt ttcttttgac atctcgatga ttatttggat
      721 cgtcgcgcta tggtcttaca gtccggatcc ggttccagag cccggcattt atctcgagga
      781 agattacgaa gcctttgtgg cgaagactcg gagcgccatg cgttcttact ttagaaagat
      841 cgtccgctga tgggttctag ctcgagtcga gcaagattat ttctaccgtt cgggacgagg
      901 agattcttgc tgtcctggtc gcatggttct cgctttggag gtatataggg atacgcgagt
      961 acgccccaca tggcgtatac ttcagcccct ttcgtacgac gttgcacttg tggttgggcc
     1021 gtggattgtg gtcctattcc ccgccaccca cccgagggtg aataagaaca tggggacaaa
     1081 gcgcgggtcg gcgagcatag tccgctggac ggcttcccgc ggcttgtccc gctaaggcag
     1141 gactcgtgat ccagctaatt ttattcctgt tgatcggggc gctgcttttt ctgtccctga
     1201 ttttccttgt ttggcggagc ccacgggctg aagagggttc ggcggccttg gttggagcga
     1261 ggcaggcgct aatagcgttg cagacgggct tgcttcccac ggaactaatc gaccggattt
     1321 ttgcccgaga cgacctcgaa tatgtccgat tgtatgctcc cagacctgtg tgcatgatgt
     1381 ttcttcgtga gcgcaaaagt gtcgcgctcg catgggctac gcagatgaga aaacaggtac
     1441 tcagcttgat gcgtttccat ctgggctcgg cacggtttta cgctaggcta agcttccgga
     1501 cggaattggt cctcgcagtt cagtttggcg gcttgctgct ggcctgccgt gcccttcaga
     1561 tgattttgta tgtcggaggc ccgtacgcgg cgcctcgcat cgtgggtgca acggctgcca
     1621 cggccgttca agtttgcaca atctcagagc ggtccctggc atttttgaat tcggcccgcc
     1681 ttggagcgct gcctgaccgc cgctcgagcg cttcattcta ggaattggac catgagctcc
     1741 ctaccccgac gactcgacgt tcaggaggcc gctgacgacg tgcgccagcg caccctttcg
     1801 gagcttccac aatcgcttga ccggcttatt taccttgcat cgatgcgcga ctacaacact
     1861 ggtctctatt atcacgatgg attggcggcc cggttcagcc aagaagtcgc ctgcgaggcg
     1921 ctggcggatt gtcaccggga agtttttcga cagctcgtgg cctgtccgct ggaaaatgtg
     1981 gtcggtcaga tggaagccta tatgaagtcg acgcatacca gtccgagtga gttcctagca
     2041 gcgtggcgca agctggaacc ctatcgcgtg gtcgtgccag tcgaggccga tcctctctct
     2101 acgcagcttc ttttttctac cttcaagatc gccttggcaa ttttggaagc tcgtctgact
     2161 cactctccgg aggaatcagg cgcatcgcca cgccggtaac ctgcccgacg atttccgctt
     2221 ccgaaggata aacgaattgg cggagcgcgc atcccgataa cggatgtggg atcagtgtga
     2281 gttgcgatcc ccgcacttcg caccacgaac acgcatagcc atcccgcagt tcgacgaaat
     2341 aaatgggccg gtcaaattcg gtgcgccatt ccgcggcttg catgtgggtc ggctggttgt
     2401 cgatctgcac aaatgatcca ggacgcagca gcggatacat cgtatagtcc tgcacaccga
     2461 tataaccata ctggcaatgg cgcacgtcca gcttctgaat caaagcgatg gggacttcac
     2521 cccatacctc caccatgcgc gcaatcaggc tggtggtctc caaattgaag cttttgtcga
     2581 accgtacagg gaaagtgatc gccttttcgg gactaggcgc ttcgaacgac gccaaatggg
     2641 tgttctgcag cggaagtgcg agctggtatc gcgacgtggc gctcaggtca atgccaaaca
     2701 cgagtagaag atcgttgaac ttcgtacgat agattacgct gaggctgtag agcttgtaga
     2761 tgctaggaac cgaattcttg ttctccaact gagtcaacca ggcgttggag atgtagaact
     2821 cttcattctg acgatcttcg gcgatgctgc ggctgaattc ttctacctcg cgcgtggtta
     2881 cgccgagtcg gttacgaagt tctctgagct gctcccctgg gcgcatacgt gtccgtacat
     2941 caaacgcttc gcaatgaaag gtcgagccgg gtgattggaa cgccatgaac aataaagctt
     3001 tcctgcccat gaatcaactg gattgaggaa gccacccgtg cgagcgtcca tcctcgttta
     3061 aatctcgctc ggggatgatc cgtaccgccc ccgcgcagcc ttgcgtccgc gaaacgcgct
     3121 ctctcgctct cgatgcagaa accgaggtct ttcgtgcttt gaggctaatg ttgggtttta
     3181 gtactgctat gctcacaatt gaaagcaact cccgtatcct cactgcaact ctcggctccc
     3241 gaaccataat ttcgggtttt tcccctcgtg cccaaaaatc atcccgaaag gatgatgatt
     3301 ccactgctgt aggcatcaag gatgggccga aatctaccgc gcaccactgc gatgatttgg
     3361 catcaagttg ggggtgtttg cgctttggtg tcctctcgta tgattgattc ccggcgcgat
     3421 atgcatctgt gccaacctaa tcaaaaggca ggatggaagg tgttgcgatg agcagcaatt
     3481 cagcagggcc gtccactgga tcccaagcgt cgggtagtga acgccgcacg gtgccgcgtt
     3541 attcgcttat cgctaccgcg gaagtgatcg agccggtcag cgaagtgcgg atttcaggcc
     3601 gcatctcgga aatcagtcgc aaaggcgtct acgtggatat cctgaatccg cttcctcggg
     3661 gcacccgcgt cactattcgc atctcgcgcg atcaaggaac cttcgtcgct accggaaaag
     3721 ttatttacgt gcaggaagga atggggatgg gcgtcgcttt tctcgatgtg gcagaagacc
     3781 agaagaatct ccttatttcc tggctcgccg aactaacggc ttagccgtca ggctgggctt
     3841 tgcccctccg aaaccatcca gtccggaggc ggttcgtgaa ctggccagaa ttccggtacc
     3901 ggccggtcga gttcgattcc cacttgggtt aaacccgcat aaggcattcc cgcataggca
     3961 atcttccccc ggatttcaac ggtgtctgtg agctttcgaa ggaccactct ctgccccagg
     4021 accaccttcg cggcgagcat taccaatgca ccgtgagcac tcacggtaac ggtgaatgtt
     4081 tcctcacgga aggcttgctg ctcggaggac tcgccccaaa tcatcaatgg aacgtccagc
     4141 agcagacgcg cactccgtcg ttctccaatc gtcgtcatga atgagccgcc cgcatccata
     4201 ttgccttacc cgggctttgc cggtcagagc caatagcgcc gcccatgccg ccaaagtaac
     4261 ggcgcctgcg attctgctgg ccgcgacgga accaccgctc gcgacagaat tcaccgctcg
     4321 cgtcagcttc gactgttggt atgcgaagtg gccaccatta tgctaggctt gacactggtc
     4381 acgatagctg acaagacagc aaaggcatga agatcaatcg caagccgtca cgcctcgcaa
     4441 caggccggat gcacatggcg gtcgaccgcc ggcgccggat cgtggcatgg atgcgcgccc
     4501 atggcgctcc ggtccgtggc ggcgagctgg ccaagcgcct tcgcgtgagc cggcaatgtt
     4561 tggtgcagga tgtggcgatc ctgcgtgcaa gcggccagga aattctagcc acgccgcagg
     4621 gttaccgcct cccgaaaggc tccagccgct cccaccgtgc cctgctcgcc tgtaagcatg
     4681 tccccgagcg aacagaagaa gagcttcaga tactggtcga caacggagtc aaggtgctag
     4741 atgtcatcgt cgagcatccg ctttacgccg aattgcgcgg ctcccttatg atcgaatcgc
     4801 gcgcggacgt gcaggatttc ttggaccgtg tgcgctcctc gcatgcatct cttctttctt
     4861 cgctaacgcg cggcgtgcat ttgcacactg tagaggcgtc acgtcctgag atgatcgcgc
     4921 gcgccaagac agccctgcgg agacgcggct ttctgctcaa gtaacgcgtg gacattttcc
     4981 atgaatgaga ctcgacggca acgaattgaa aaagcgctct gtcgcggagc cagcctatcg
     5041 atcgacaatc cgcagtattg tcaggccgtc cgcacgttct ccgatttctt gtttcaggcg
     5101 gaccttggta cgcgagacct tactgcggaa gccgtaggaa aaagaagcct tggcgcaaga
     5161 gcgacagtgc tggcacgcga aggcggaata gctgccggtc tggaagaatt cgctctgttg
     5221 ctgcgctcgc atggcgtgcg cgtggaattc gagaaaaaag atggcgacgt gatacgcccg
     5281 ggcgaagcat tgctatacgc cgaaggagat gaaataaacc tgctctcgct cgaacgagtc
     5341 ggtctgaatc tcgttcaacg aatgagcgga atcgccaccg cggcacgatg cctgcaacag
     5401 cgcgtcaacc agcgcggctt cactgcgctg gtagttggca ccaggaaaac tcactggggc
     5461 ttgctcgata aacgcgcact gcatctgggc agcggcggca cccaccgcct cggcttgggc
     5521 gatgcgattt taatcaagaa caatcatctg gcgcttttgg caggcacgga agcggaagcg
     5581 gcgcctctgg cgattgaaaa ggcgtggaca tttcggggcg aagcagcctt tatcgaagtg
     5641 gaagtccgcg gcgaagatgc cgcgcgcgcc gccgcgcaga catttcgccg cctgcgggcc
     5701 gataccgcgc acgattaccc atgtctgctg atgctggaca acatggctcc cggcgagatc
     5761 gcggcgattc tcgatgcgct ccggcgtgaa aatttatgga gcgacgtgct gatcgaggcg
     5821 agcggcggaa tttcggaagc aaacctcgag gcgtacgccg cttccggagt ggatgcgata
     5881 tcggtgggcg ctttgacgca ctcggttcgg gcgctcgatg tgtgccaacg aattttgtaa
     5941 ttatcttaat ccatggagag caaaaatatg gcaagcgcac ctacgcagga gttttttgaa
     6001 gcacgctaca ctcttcccgt cgttgaacag atcgacgata ccgctgacga gattcgcgca
     6061 aagcaggagc gcgtccgtgc gctttgcgtc gaacgcaatg ctgttctgct ggctcaccat
     6121 tatcagcgtc ccgaggtgca ggaagttgcc gacgctgttg ccgattcgct gaaactttcg
     6181 caggccgccg cgaagaccaa tgcggacgtg atcgtcttct gcggagtaca tttcatggcg
     6241 gagactgccg caattttact tcccgataaa accgtcctgc tgcccgatct gcgcgccgga
     6301 tgctccctcg ccgcatcgat cacgcccgaa gaactgctaa agtggaaggc aaaattgccg
     6361 gatgccgtgg tggtttctta catcaacact tcagcggccg tgaaagccga gagcgactat
     6421 tgctgcacgt cggcaaatgc agccaaggtc gtccgctcga ttccgtccga taaaaccatc
     6481 ctatttctgc ccgataaatt tctggcggat gttgtggcgc ggcaaacagg tcgcagcaac
     6541 atcgttgcgt atcccggcta ctgccacgtg cacaagacga tccgcccgga gcaggttgtg
     6601 gaaatcctcg aggaacatcc cgatatcgaa ctgctcctgc atcctgagtg cggttgtgtc
     6661 agttcctgca tggccaaagc ggtggacggg accttgccgc aggatcgcac ctttttcctt
     6721 tccactgaag gaatgctcaa gcatattggc gaatccgatg ccaccgaatt cgcggtgggc
     6781 accgagctgg gaatttttca tcagctcaac aaaaaattcc cacagaagac gtttcacgct
     6841 gtgaatccca acgccgtttg cgctttcatg aaaaccatca cgctcgacag agtgattcaa
     6901 tcgctcgaga ccatgactcc tcaaatccgc gtgccggaag atatcgcgcg caaggcgaag
     6961 cgtgccatcg atcgcatgct cgcgctggca tagggcgcgc gatatgttga aaatcggact
     7021 cctcggcgtt ggcgccattg gcaaaaccat cgccaccgct ctcgatcaaa aacaagtgga
     7081 tgccgaactt gtcgcgctat ccgatcagga ctgcgcgcgc gccgagcaac tttcgtcgga
     7141 actttcaaat cagccgcccg tgttgtccgt cgaagaaatg attgaccgct gcgaattggc
     7201 cgtcgaagcg gcgagccagg ccgcgcttcc ggacttcgtg cccaaggcgc tcgcgcgcgg
     7261 gcgcgacatg ctgatcatga gcgtcggcgg gctgctgggt cgtcaccaat ggtttcgcga
     7321 ggcggaaaca aaaggctgcc gaatctatgt tccgtcaggg gccatcgcgg gacttgacgg
     7381 catcaagtct gcctcgattg gccgcatcga ctccgcggaa ttgaccagcc gcaagcccgt
     7441 gaaggcgctg gccggctcga aatacatcct cgatcacggg attcaactcg atggcatgaa
     7501 agaagacacc gtcatcttcg acggctcggc ggaagaagcc gcccaagcgt ttcccgcaac
     7561 ttcgaacgtt gccgcgtcgc tgcgtctcgc cgtcgatcgg gcagcggtgc gtgtgcgcgt
     7621 ggttgccgtg cccggaggca gcgaaaacgt gcacgagatt cgcgtccagg gcgaattcgg
     7681 ccgcctctcg atgcgtgtgg aaaacgtgcc ttcgaaaacg aatccccgca cctctcagtt
     7741 ggccgcgttt tcggccatcg ccacgctgaa gaatctcacc cgatcgttgc gagtgggcac
     7801 ctagcagcac ctcgcggcac ctgacaaagt cacaatcgag tatcctgacc tcactcgtgc
     7861 ccaagcccaa atcagcagcg gttcttcatg aaagctttct tgtaagcatc gccttgaagg
     7921 gacttggcgc gctctccgag gtcatcgcgg gaatcgtctt actgaaaatt tcacc
//
LOCUS       DATNDT010000224         8017 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1785817, whole genome shotgun sequence.
ACCESSION   DATNDT010000224 DATNDT010000000
VERSION     DATNDT010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8017
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1785817"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="VN788_07260"
     CDS             complement(<1..710)
                     /locus_tag="VN788_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU20362.1"
                     /translation="MKTPLNCFRLAAIASAFAILPGMLPAQGLDPADLLHPPADSWPL
                     YHGDYSGMRHSHARQITPQNVGQLALAWAFQTNERAELKCSPLLVNGVLYFSVPDNVW
                     AVDAHSGREIWHYSYHPNPGLHIGHRGVAMYKGWIYFETPDDHLLCLDARNGKVHWDV
                     VLADVSQGYWATMAPLVVGNHVLAGISGDFDNVRGFLRSLDPETGKTQWTWYATPPAG
                     TPNSTTGGTTWMTGTYDP"
     gene            complement(723..1541)
                     /locus_tag="VN788_07265"
     CDS             complement(723..1541)
                     /locus_tag="VN788_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU20363.1"
                     /translation="MIGTSFLSGGRARACLFGAAAGILFAIPQARAQRPTTAGVHIKA
                     QTYAPDVVERGHQRFEQNCAFCHGREATGGESGPDLTHSGVVAADVHGDKIGNVVRNG
                     LPGGMPKFDFSQEQIVELAAFIHTEAAKANSDEAQRRKIGLSDLLTGNVADGKQYFDS
                     KCAGCHSPTGDLAGIAEKYKNPIQLETRMLYPWGAQAKVTVTLPSGQTVAGQLIHANE
                     FTVALRDSDGWYHSWPASEVKYSIDDPAEAHVKLLPQYSDADVHNLFAYLNTLR"
     gene            complement(1551..3197)
                     /locus_tag="VN788_07270"
     CDS             complement(1551..3197)
                     /locus_tag="VN788_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acido-empty-quinoprotein group A"
                     /protein_id="HXU20364.1"
                     /translation="MKMNRFLLPASLGLLPLALTAQMLNPALLTKPATNAWPTYNGDY
                     SGRRFSPLTQINQSNVKGLTLAWVHRFIGGPGFGNVIVGGEGPGDAPANNFFGNSIKA
                     TPLMVNGVLYFATPDNAWAVDARSGRELWHYFWKTTGGIHIGNRGMGMYGNWLFFETP
                     DNYLVSLEAATGKERWHVKIADVKQEYFSTPAPIVVGNHVIVGTGGDSLDVPGFLEAH
                     DPETGAVQWKWWSEPLKKGDPGSESWPDEYSMRHGGGMTWIPGTYDPELHLYYIGTGN
                     PNPVESGHSRKGDDLFTCSIVALNVDTGKMAWYFQASPHDTHDWDSAQTPVLFDGVFD
                     GKPRKMLAQANRNGYFFVLDRTNGKNLVTAPMIKTMNWSLGINSKGQPIGNPEKEATI
                     PGVLVSPDSSGATNWPPPSFDPETGLFYVGIDESFSVFYLTDTDPHPEGYAAAERGAG
                     SYGGSLKAIDYKTGKAVWTHQYPSGGGPVGILTTAGHLLFTGDGAEHLIAYDPANGKI
                     LWHAGMASGVSNGPETYMLDGQQYLVVGAGDSLYAFTIEQ"
     gene            complement(3194..4012)
                     /locus_tag="VN788_07275"
     CDS             complement(3194..4012)
                     /locus_tag="VN788_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU20365.1"
                     /translation="MRHRKKIFGIAMALFASSAVQLAAQGPGKRGPTEDPASVERGEK
                     QFVAACSFCHGVHAKGGEGGPDLLRSVLVLDDTHGDKIGPVILNGRPGTAMPKFAMTP
                     EQISDIANFLHHSINAAAERGGYQILDIVTGNAAAGKAYFNGAGKCNTCHSVEGDLKG
                     IGAKYDPSTLQGKFLMPRGRSWRRRGKPTKPEAPVTATVTLPSGQTFSGTLDHIDDFN
                     VALTDASGDYHSFVRKGDVPKVELHDPLQFHKDMLMKYTDTDIHNLTAYLVTLK"
     gene            complement(4063..5691)
                     /locus_tag="VN788_07280"
     CDS             complement(4063..5691)
                     /locus_tag="VN788_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU20366.1"
                     /translation="MLSVNNVSMRYGARILFEDVTTTFLAGRRYAISGPNGAGKSTLM
                     KILTGEIDPSKGSVTRPKKLGVLRQDQFAFDKYRVLDTVVMGNAPLWDALRERETLYE
                     KDPDALTDEDGMRLGELEGIVGDEGGYTAETDAAVLLDGLDIPESLHERKMGELQGGQ
                     KVRVLLAQALFGNASALLLDEPTNNLDLESVHWLQDYLRAYEGCLIVISHDRHFLNDV
                     CTHTADIDYQTVITYTGGYDEMVLAKTQIRARIEAGNNQREKKIAQLNEFIARFSAGT
                     RSSQVMSRKKEVERLQTSELAKSNIQRPFIRFEMKRPSGRHPLEVKALSKSYDGVNVI
                     QRFSTSISRGERIALIGRNGAGKTTMLKSLLRNAPGFIDAEDQAFPVDSGVVTWGHEV
                     SVGYFSQDHQQSIRGGAPAIEWLHEFDPQASQAELRGVLGQMLFSGDDALKPTSALSG
                     GEAARIIFCRLMLQKPNFLVLDEPTNHLDLESINALNIALQRYEGTVLLVTHDHDVID
                     EVATRIWHFENGQIEDFKGPYAEYLIHAGEAVTV"
     gene            complement(5783..7282)
                     /locus_tag="VN788_07285"
     CDS             complement(5783..7282)
                     /locus_tag="VN788_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HXU20367.1"
                     /translation="MSTSAERPGEFPFTRGIYPDMYRRRLWTMRQYAGFGTPEQTNQR
                     YRYLLSQGITGLSVAFDLPTQMGMDSDHPLASGEVGRVGVSICSLADMERLFDGIRLD
                     TITTSMTINSTAAILLSLYVHVAKKQGANLRDLSGTVQNDILKEYIARGTYIYPPRPA
                     MRIVTDLFAWAERELPAWNTISISGYHIREAGSTAVQEVAFTLANAIAYIEAALKAGL
                     AVDSFAPRLSFFFNSHSNFLEEIAKFRAARRLYARIMKQRFGARNERSTMLRFHAQTA
                     GSTLTARQTDVNVVRVSLQALAAVLGGAQSLHTNGRDEALSLPTEESARLALRTQQII
                     AYETGVTNTADPCGGSYEIERLTDEIEHGANNYIERIDAMGGTLAAIENNFIQTEIQN
                     AAYDYQRQIERQDKIVVGVNRFEQSEPESPKTFHLDPALERQQVERLREMRAGRSASV
                     VADRLRSLEHAARGTENLMPHLLSATESYATVGEISDRLRAVFGQYREN"
     gene            complement(7279..7533)
                     /locus_tag="VN788_07290"
     CDS             complement(7279..7533)
                     /locus_tag="VN788_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20368.1"
                     /translation="MPLLARKFVRAFLPGVIKPLHVLWNEIIGFLFLVLAVFILFGTI
                     RRTYGKPDSFFLMIAGIAFGLLLAGFGISSFLRARKISRS"
     gene            7695..8012
                     /locus_tag="VN788_07295"
     CDS             7695..8012
                     /locus_tag="VN788_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20369.1"
                     /translation="MDDRLKELMQELGNAINESLSDSDRIAGAIGEIKRAGYDVFLVL
                     EATIGFNKREEGADEDSEEGEGATAEPPSKRKFESTGKIKLTSQDQKFLRALKIAIDE
                     ENR"
BASE COUNT         1702 a         2251 c         2361 g         1703 t
ORIGIN      
        1 tttggatcat aggttccggt catccatgtc gtgccgccgg ttgtcgagtt gggagtgccg
       61 gcaggcggag tggcgtacca ggtccactgt gtctttccgg tttccggatc gagggaacgc
      121 aagaagccac gcacattatc gaaatcgccc gagatgccgg ccagcacatg attgccgacc
      181 accagcggcg ccatggttgc ccagtagcct tggctcacgt cggcgagaac gacatcccaa
      241 tgcactttgc cgtttctcgc atcgaggcat aggagatggt cgtccggagt ctcgaaataa
      301 atccagccct tgtacattgc gacgccgcga tggccgatat ggaggccggg gttcggatgg
      361 tagctgtaat gccaaatctc acggccggag tgcgcgtcca ccgcccagac gttgtccggg
      421 acgctgaagt aaagaacgcc gttcaccagc aacggcgaac atttcaattc ggctcgctca
      481 ttggtttgaa acgcccatgc gagggcgagt tgcccgacgt tttgcggagt aatttgccgc
      541 gcatggctat gtcgcatgcc ggaataatcg ccgtgataaa gcggccagct atcggcggga
      601 ggatgcaaga gatcggcggg atcgagtccc tgagccggta gcattccggg caggatggca
      661 aaagcgctcg cgatcgcggc aagacggaaa cagttcagcg gtgtcttcat cgggggttca
      721 atctaacgca acgtattcag atacgcgaac aggttgtgaa cgtcggcgtc ggagtactgt
      781 ggtaacaact tgacgtgggc ttcggcggga tcgtcgatgc tgtatttcac ttcggatgcc
      841 ggccaggagt gataccagcc atccgagtcg cgtaaagcga ccgtgaattc attggcatga
      901 attagctgtc cggcgacggt ttggcccgac ggcagagtga ctgtgacttt tgcctgcgct
      961 ccccatggat acagcattcg cgtttcgagt tgaatggggt tcttatattt ctccgcgatt
     1021 ccggcaagat cgccagttgg cgaatggcat ccggcgcatt tactgtcgaa atactgcttt
     1081 ccatcggcga cgttaccggt cagcaggtcg gataaaccga ttttgcggcg ctgcgcctcg
     1141 tcggaattcg ctttggccgc ttccgtatgg atgaacgcgg caagctcaac gatttgttcc
     1201 tggctgaaat caaatttagg catgccgccg ggcagtccgt tgcggacaac gttgccaatt
     1261 ttatcgccat gcacatcggc ggcgactacg ccggaatgcg tcagatcggg tcccgactcg
     1321 cccccggttg cctcacgccc gtggcagaaa gcgcaattct gctcgaagcg ctgatggccg
     1381 cgctcgacga catcgggagc gtaagtttga gccttgatgt ggacgccggc cgtggttggg
     1441 cgttgcgccc gggcttgcgg tatcgcgaac agaattcccg cagccgcgcc aaagaggcac
     1501 gctctcgcgc ggccgccaga cagaaaggag gtcccaatca tctcgatttg ctactgctcg
     1561 atggtaaagg cgtagagcga atccccggcc ccgacgacga gatactgctg accatcgagc
     1621 atgtacgtct ccggcccgtt gctgaccccc gaggccatgc ccgcgtgcca caggatcttg
     1681 ccattggccg gatcgtacgc aatcagatgt tcggcgccgt ctccggtgaa cagcaagtgt
     1741 ccggccgtgg tcaagatgcc gaccgggcca ccgccggatg ggtattgatg ggtccagact
     1801 gctttgccgg tcttgtaatc gatcgcctta agagatccac cataggaccc cgcgccgcgc
     1861 tcagccgccg cgtacccttc gggatgcggg tccgtatcgg tgagatagaa aacgctgaac
     1921 gattcgtcaa tacccacata gaaaagaccg gtttccgggt cgaagcttgg aggaggccag
     1981 ttggtcgcgc cgcttgagtc cggcgatacg agcacgccgg gaatcgtggc ttccttttcc
     2041 gggttcccga tgggctggcc tttactgttg atgcccaacg accagttcat cgtcttaatc
     2101 atgggagccg ttaccagatt cttgccattg gtgcgatcaa gcacaaagaa atagccgttg
     2161 cggttcgctt gcgcgagcat cttacgcggc tttccgtcga atactccgtc gaacaggacg
     2221 ggtgtttggg ccgaatccca atcgtgcgtg tcgtgcgggg aggcttggaa gtaccaagcc
     2281 atcttcccgg tgtcaacatt cagagcgacg atggaacatg tgaaaagatc gtctcccttg
     2341 cggctgtggc cggactcgac gggattcgga ttgccggttc cgatgtagta caggtgcaac
     2401 tccggatcgt aagtgccggg tatccaggtc atgccgccgc cgtggcgcat ggaatattca
     2461 tcgggccaac tttccgagcc cggatcgccc ttcttcagcg gctcactcca ccatttccac
     2521 tggaccgcgc ctgtctctgg gtcatgcgct tcgagaaaac cgggaacatc cagggaatcg
     2581 ccgccggtac cgacgatcac gtgattaccg acgacgatgg gcgcgggcgt tgagaagtac
     2641 tcctgcttga cgtcggcgat tttcacgtgc cagcgctcct tgccagtagc agcttcgaga
     2701 gagacaaggt agttatcggg agtctcaaag aacaaccaat tgccatacat gcccatgccg
     2761 cgattgccga tgtgaatgcc gcccgtggtc ttccagaagt aatgccacag ctcgcggcca
     2821 gaccgggcgt ccacggccca ggcattatcg ggagtggcga aatagaggac gccgttcacc
     2881 attagcggcg tcgctttaat ggaattgccg aaaaaattat tggccggagc atcgccgggt
     2941 ccttcgccgc cgacaatgac gtttccgaaa ccgggaccgc cgatgaagcg atggacccaa
     3001 gcgagcgtca ggcccttgac gttcgactga ttgatttgcg tgagcgggct gaagcggcgt
     3061 cccgaataat cgccgttata ggttggccat gcattcgttg cgggtttcgt tagcaatgcg
     3121 ggattcagca tctgcgcggt gagcgccagc ggcaagaggc cgagcgaagc ggggagcaga
     3181 aaacgattca tcttcatttc agagtcacca gataggccgt gaggttgtgt atgtcggtgt
     3241 ccgtgtattt catcagcata tctttgtgga attgaagtgg atcatggagc tccactttcg
     3301 ggacatcgcc tttgcgtacg aaggaatgat agtcgccgct cgcatccgtg agcgcgacgt
     3361 tgaagtcatc gatgtgatcc agcgtgccac taaatgtttg gccagacggc aatgtaacag
     3421 tcgcggtgac gggcgcttcg ggctttgtgg gtttaccccg gcggcgccag cttcgtcctc
     3481 ggggcatcag aaacttgccc tgcaatgtcg agggatcgta ctttgctcca atccctttca
     3541 gatcgccttc cacggaatga cacgtattgc atttccctgc gccgttgaag tacgccttgc
     3601 ccgcggctgc gttgccggtc acaatgtcga gaatctgata acctccccgt tccgcagccg
     3661 cgttgatgga atgatgaagg aaattcgcga tatctgaaat ctgctcagga gtcatggcga
     3721 atttcggcat agcggtgccg gggcggccgt tcaaaatcac cggtccaatc ttgtcgccgt
     3781 gagtatcgtc caaaaccagc acagaccgta gcaggtcggg tccaccctcg ccacccttcg
     3841 cgtgtacgcc gtggcagaag ctgcaagccg cgacaaactg tttttcgccg cgttcgaccg
     3901 aggccggatc ttccgttggg ccgcgcttgc ccggcccctg cgcggcaagt tgcaccgcgg
     3961 aactggcaaa cagagccatt gcgatgccaa aaatcttctt tctatgacgc actcattcct
     4021 ccacgtggaa aatgcccgat attacaggac ggcggcgccg gatcatacgg ttacagcttc
     4081 cccggcgtga atcaagtact cggcgtaggg ccccttgaaa tcctcaatct gcccgttctc
     4141 gaaatgccag atacgagttg ccacttcgtc gatcacatcg tgatcgtgcg tgaccaacaa
     4201 cacggtacct tcatatcgct gtagcgcgat gttgagagcg ttgatcgatt cgagatccag
     4261 gtggttcgtc ggctcgtcca gcaccagaaa attcggcttt tgcagcatga gccggcaaaa
     4321 gatgatccgc gccgcttcgc cgcccgatag agcgctggtg ggtttcagag cgtcatcgcc
     4381 gctgaatagc atctgcccaa gcacgccgcg cagctcagct tgcgaggcct gcggatcgaa
     4441 ttcgtgcagc cattcaatgg ctggcgcgcc gcctcgaatc gattgctggt gatcctgcga
     4501 gaagtatcca accgagacct cgtgtcccca tgtcacgacg cccgaatcca ccggaaacgc
     4561 ctggtcctca gcatcgataa aacctggcgc gttccgcagt agagacttga gcatggtcgt
     4621 tttacccgcg ccgttgcggc cgatgagcgc aatccgttcg cctctgctaa tcgacgttga
     4681 gaagcgctga atcacattga cgccgtcgta agatttcgag agcgccttca cttccagcgg
     4741 atgccgcccg gacggacgct tcatctcgaa acgaatgaac ggacgctgaa tattcgattt
     4801 ggccaactcc gaagtttgca gccgctcgac ttcctttttc cgggacatca cctgagatga
     4861 ccgggtgccc gccgaaaaac gggcgatgaa ttcattcaac tgggcgattt tcttttcgcg
     4921 ctgattgttg ccggcttcaa tgcgcgcccg gatctgggtt ttcgcgagca ccatctcgtc
     4981 atagccgccg gtgtacgtga tgacggtttg gtagtcgatg tcggccgtgt gagtgcagac
     5041 gtcattgagg aaatgccggt cgtgcgagat cacaatgagg cagccttcgt aagcgcggag
     5101 ataatcctgg agccagtgaa ccgactcgag atccagattg ttcgtcggct cgtccagaag
     5161 cagcgcgctc gcgtttccaa acaacgcctg agccagcaac acccggactt tctggccgcc
     5221 ttgcaactct cccatcttcc gctcgtgtaa cgattcggga atatcgagac cgtcgagcaa
     5281 aacggccgcg tcggtttccg ccgtatatcc gccctcgtct ccgacaatcc cttccaattc
     5341 tccgaggcgc atgccatcct cgtctgtcag cgcgtccgga tctttttcgt aaagcgtttc
     5401 ccgctcgcgc agcgcgtccc acaggggagc gttgcccatg acgacggtat cgagcacgcg
     5461 atacttgtcg aacgcaaact gatcctggcg cagaacgccg agctttttcg gccgggtcac
     5521 ggaacctttg gacggatcga tctcgccggt caggatcttc atcagcgtgg acttgcccgc
     5581 gccgttcgga ccgctgatag catagcggcg tccagcgaga aacgtggtag tgacatcctc
     5641 aaacaggatg cgggcgccgt agcgcatgga gacgttgtta acggatagca atgcgaaaac
     5701 tctgagttcc tgggttgccg ggattatacc ggtacggcaa ggactgacaa ttcagcttat
     5761 tcttcagtat agcgtcttca tattaatttt cccggtattg gccaaagacc gcgcgcaaac
     5821 ggtccgagat ttcacctacc gtcgcgtacg attcggtcgc actcaggaga tgaggcatta
     5881 gattttccgt gccgcgcgct gcgtgttcca gcgagcggag gcggtcagcg acaaccgaag
     5941 cgctacgccc ggcccgcatc tcgcgaagcc gctccacttg ctggcgctcc agcgcgggat
     6001 cgagatggaa tgtttttgga gattccggtt cgctctgctc gaatcgattt actccgacga
     6061 cgatcttgtc ctgccgctcg atctgccgct gataatcata agcggcgttt tgaatttcgg
     6121 tctggatgaa attgttctca atggccgcga gagtgccgcc catggcatcg atgcgctcga
     6181 tgtagttgtt tgcgccatgc tcgatttcat cggtcagccg ctcgatttca taactgccac
     6241 cacagggatc ggccgtattc gtcacgccgg tttcgtaagc aatgatctgc tgtgtgcgga
     6301 gagcgagacg cgcggattcc tcggtgggca gagagagcgc ctcatcgcgc ccgttggtgt
     6361 gcagggattg cgcgccgccg agcacggcag ccagcgcctg gagcgacacg cggaccacat
     6421 tcacatccgt ttgccgagcc gtgagagtcg agccggcggt ttgcgcgtgg aaacggagca
     6481 tcgtcgaacg ctcgttcctg gctccgaaac gctgcttcat aatgcgcgca taaagccgcc
     6541 gggcggcgcg gaatttcgcg atctcctcta gaaaattcga atgggagttg aagaagaagg
     6601 aaaggcgggg ggcgaaagaa tcgacagcga gcccggcctt cagcgcggct tctatatacg
     6661 cgatggcgtt ggccaatgta aaggcgactt cctgcaccgc ggtcgagccc gcttcacgaa
     6721 tgtgatatcc gctaatcgag atcgtgttcc atgccggcag ctcgcgctcg gcccaggcga
     6781 acaggtccgt gacaatccgc atcgcgggac gcggcgggta gatgtaagtt ccgcgcgcga
     6841 tgtattcctt caggatgtcg ttctgaacgg taccggacag gtcgcggaga ttcgcgccct
     6901 gcttcttcgc gacatgaaca tagaggctca gcaggatcgc cgccgtcgaa ttgatcgtca
     6961 tcgacgtggt gatcgtatcg aggcgaattc catcgaagag ccgctccatg tccgcaaggg
     7021 agcagatgga taccccaacg cgtccgactt cgcccgaggc cagcggatga tccgaatcca
     7081 tacccatttg cgtcggcaaa tcgaaggcga ctgaaaggcc cgtgattcct tgcgagagta
     7141 ggtaacggta acgctggtta gtttgttcag gcgtgccgaa gccggcatac tggcgcatag
     7201 tccagaggcg gcggcggtac atatcgggat agattccgcg agtgaacggg aactcgccag
     7261 gccgctcggc agacgtgctc atgaacgtga aattttacgc gcacggagga aagaagagat
     7321 gccgaagccg gccaggagaa gtccgaaggc gatacctgcg atcatcagaa aaaagctatc
     7381 cggtttgccg tatgtgcggc gaatagtacc gaacaaaata aatacggcta gtaccagaaa
     7441 aaggaacccg atgatctcat tccagagcac gtgcagcggt ttgattactc ccggcagaaa
     7501 cgcgcggaca aattttcggg caagcaaagg catgaactta tcttcatttt agccttaagg
     7561 taccggtgga gttcagccga tggaagacag gaggccaccc taccgcgagg caaagactga
     7621 aacttcctca tacggcgcaa catcaagaag ggtggatggt atcctgataa tggcccgtta
     7681 gaaaggggca attcttggac gaccgactga aagagttgat gcaggagctc ggtaacgcaa
     7741 taaacgagtc tctctcggac tccgaccgga ttgcgggcgc gatcggcgag attaaacgag
     7801 cggggtatga cgtgttcctc gtgctcgaag cgactattgg ttttaacaaa cgtgaagaag
     7861 gagcggacga agacagcgaa gaaggcgaag gagctaccgc ggagccaccg tccaagcgta
     7921 agtttgaatc gacaggcaag atcaaactga cttctcaaga tcagaagttc ctgcgcgcgc
     7981 tgaaaatcgc aatcgacgaa gagaaccgct gatattg
//
LOCUS       DATNDT010000225         6695 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1824489, whole genome shotgun sequence.
ACCESSION   DATNDT010000225 DATNDT010000000
VERSION     DATNDT010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6695
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1824489"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..540
                     /locus_tag="VN788_07300"
     CDS             <1..540
                     /locus_tag="VN788_07300"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HXU20370.1"
                     /translation="VGLKVEDRVYSDLPVDVVVRQSPPPKMRVKAGADEHVVLSLGQQ
                     KVTIPQVEGMSERLARIQLLRSGMQLGEESTVYLPGATADTVLEQDPEPGTANATSPH
                     ANILVAAEPPTAAYVMPQLDGSTLTSAEAKLATAGLKVSKITLEQAPGAQHGVVLDQV
                     PTRGTRVDASVQIELHVAE"
     gene            680..1366
                     /gene="rpe"
                     /locus_tag="VN788_07305"
     CDS             680..1366
                     /gene="rpe"
                     /locus_tag="VN788_07305"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXU20371.1"
                     /translation="MAYERIEIAPSILAADFARLGEQIREAEAGGADVIHFDVMDGHF
                     VPNISIGIPVLKSIRKATRLPLDVHLMIDNPEQYISAFVRAGANRVLVHQEATPHLDR
                     ALEMIREAGAEAGAVINPATPVAMLTDVLEKVDTVLVMSVNPGFGGQKFIPRAAEKVA
                     QLNELRTKRRVSFRIAMDGGIELENISELARAGANTFIAGTSIFHSPDPANAVRKMRA
                     AALEATRQMV"
     gene            1388..2887
                     /gene="bamD"
                     /locus_tag="VN788_07310"
     CDS             1388..2887
                     /gene="bamD"
                     /locus_tag="VN788_07310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HXU20372.1"
                     /translation="MKGNRFVVTAGAILITAFSGASAFGQGRGKIKVIKPKETAKPAV
                     QPSSEPDKVLYDRAMVDLKKKHYEEERLTLETLINTYPDSEYLDKAKLAVADSYYTEG
                     GVSNLTQAIGEYKSFIVFFPFDDKAPYAQMQIGMAHYKMMEKSDRDNSEALSAEAELQ
                     TFLLKYPNDKLTPFAEQKLRDVQEVLADGEYKVARFYYLRPDYQASAARLVELTERYP
                     LYSESDEALWMLSSIYQKAKVAEAKNEDLRNHYADLEGKVLDRLVAEYPLSARAAAAK
                     EELKGMGMDVPAADPAAVARMKKQDAYVKAHRESMFKAPLDIMKSGPDFSMAAQSGMP
                     NLTPPADSISAKDILSPSNSGPTFNLAANAGSSSGGTDLSGSLPVTDANDAAAASPAA
                     SDANANGIVSAPATSSAAADPGSAPSSNNPVGEVDAPTTAASDTPSAPATTAAASTDT
                     PGTSTVAGQSEANPQHASNTSPDSSSSSKPNKNESSSKKKKGLGKLNPF"
     gene            3140..5236
                     /locus_tag="VN788_07315"
     CDS             3140..5236
                     /locus_tag="VN788_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU20373.1"
                     /translation="MPKILVADDNTNIQKMVSLAFEERGIDVVSVGNGEAAVRRIPDM
                     NPDLVLADIFMPVRNGYEVCEFVKKDERFAHVPVILLVGAFDPLDEKEARRVGADGVL
                     KKPFVPPDPLIAMVISALEKNPKIAAEMAKAREVAPPEREPMPAILEAPARAEVKPLP
                     EFPEPSPEEAALVYGFGSGKRGKENPAEQDLAPVAPDADEEEETEEDFEATAPSHNWR
                     RSAMDFEVPEETSKSPFALDDESPTAMFPSEKDVPPKHVRKKEYRPMVDTEVATRVAP
                     FASLMPPPPPAPLPAARLSDQLSGKAPASSVPDFAPPSPAPVAPANEAHDSFLTATML
                     GTMPAPFSIPAVEPPQSAPVSMSPASPVPNWKAPESELPAASIFTEQNKPTDNGETSM
                     AREITPITPAVEFGTATSFATQGAHWMDAMTEALKDTRSDASETADPAPAVKMDLDAP
                     LPTTPAGSVPALVAAPEATSRAVSEELVVSPEPIQAEDEEFFADENEGASDWLPVVSA
                     ETKKASSVTDEPVLETTGPVPVRHLAADPELVAEPDDSGPISKDPALVEPPPVHVTTE
                     PLLVDEDSEPVSSQYDKHEEETAPAFVFEAPAASAVEHVEANASASEEPAGERIPTML
                     PPNREVLAEIPFLSPPAEFLAPKAEEATPAVHDVDAVVQKVLDKLEPHLRDLLSQGVK
                     PLVENILQSETMKKRR"
     gene            5347..>6695
                     /gene="valS"
                     /locus_tag="VN788_07320"
     CDS             5347..>6695
                     /gene="valS"
                     /locus_tag="VN788_07320"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HXU20374.1"
                     /translation="MAEIPKAYEPQEIEERWAKAWFDEKLFRAENSADGPAFSIALPP
                     PNITGSIHIGHMLEHTQIDMLVRWRRMRGYRTLWLPGMDHAGIGTQFVVERELAKEGI
                     RRQDLGREEFERRIWQWKEKSGGAIKQQMIRLGASCDWTREKFTLEPALYRAVIEEFL
                     RLYHDGLIYRARYIVNWCPRCQTAVSDLEVVHVERPGHLWHLRYPLVETPNEFVVVAT
                     TRPETMLGDTAVAVHPDDERYKKLVGRNVLLPLMDREIPVIADTYVDREFGTGVVKIT
                     PAHDPNDFEVGKRHNLPQIDVMTNDGHMSPAAGKYAGLDRFEARKRVVEDLKALGLLE
                     KIEDITHSVGVCDRCKSEIEPRISTQWFCKMRPLAEPAKKAVVDGVIEVVPDNQRTIL
                     LNWLDNIRDWCISRQLWWGHRIPVWHCDNCHEMVPATDSSVELFEGHARAAGVPKKCP
                     "
BASE COUNT         1491 a         1782 c         2109 g         1313 t
ORIGIN      
        1 gtggggctta aggtggagga tcgcgtctac agcgatcttc cggtggatgt tgtggtgcgg
       61 cagagtccgc cgccgaagat gcgcgtgaag gcgggcgcgg acgagcacgt tgtgctaagc
      121 ctcgggcagc agaaggtcac gattccgcag gtcgaaggaa tgagcgaaag gctcgcgcga
      181 attcagcttc ttcggagcgg aatgcagctc ggcgaggaat cgacagtcta tttgccagga
      241 gcgaccgcgg atacggtgct tgagcaagat cccgagccgg gaacagcgaa tgcgaccagt
      301 ccccatgcaa atatccttgt agcggcggaa ccaccgacgg cggcttacgt gatgccgcag
      361 cttgacggca gcacgctaac gtcggccgag gcgaagctcg cgacggcagg gctgaaggtt
      421 tcaaagatca cgttggagca ggcgccaggg gcgcagcatg gtgtggtgct cgatcaggtg
      481 ccgacgcgag ggacgcgcgt agacgcgagc gtgcagattg agttgcacgt ggcggagtga
      541 ttggtgtgaa caggcaggga cctcgggctg ggttccgaat ttagaacgca tcaattccaa
      601 ctgcgagctt aaatccagac ggaaatcgct gtagcgcctg caaaatcggc catttcgtat
      661 gctaaactgc cgcttaacca tggcgtacga acggattgaa attgcgcctt cgattttggc
      721 ggcggatttt gcgcgattgg gcgagcagat ccgcgaggct gaggccggcg gcgccgacgt
      781 gattcatttt gacgtgatgg acggtcattt cgtcccgaac atctctattg gaatccctgt
      841 tttgaagtcg attcggaagg caacccgcct gcctctggac gtgcatctca tgatcgacaa
      901 cccggaacag tacatcagcg cgttcgtacg cgccggggca aatcgtgttc tggtccacca
      961 ggaggcgacg ccccatctgg accgggcgtt ggagatgatc cgcgaggccg gagcggaggc
     1021 gggcgcggtg attaatcccg ctacgccggt ggcaatgctg accgacgtgc tagagaaggt
     1081 agacacagtg cttgtgatgt ccgtcaatcc gggattcgga ggccaaaagt ttattccacg
     1141 ggcggccgaa aaagtggcgc agttgaacga gcttcgcacg aagcggcgcg taagcttccg
     1201 aatcgcgatg gatggcggca ttgagttaga aaatatttcg gaattggcgc gtgccggcgc
     1261 gaacacgttc atagcgggga cctcgatctt ccactcgccc gatccggcga atgcggtccg
     1321 caaaatgcgc gcggcggctt tggaagccac gcgacaaatg gtctgaaacc agggtagagg
     1381 ggcgttcatg aagggcaatc gattcgttgt aaccgcaggg gcaatcctca tcacggcttt
     1441 ttccggcgcc agcgctttcg ggcaaggccg gggcaagatc aaggtaatca aaccaaaaga
     1501 aacagcgaag ccagcggtgc aaccttcgtc tgagccggac aaagtactct atgaccgagc
     1561 gatggtggac ctgaaaaaaa agcactatga ggaagagcga ttaaccctgg aaacgctgat
     1621 taacacctac cccgatagcg aatacttgga caaagccaaa ctggcggttg cggattcgta
     1681 ttacaccgaa ggtggagtat ctaacctgac gcaagccatc ggcgaatata agagtttcat
     1741 tgttttcttc ccgtttgacg acaaagctcc ttacgcgcag atgcagatcg gcatggcgca
     1801 ttacaagatg atggaaaagt ccgaccggga caattccgaa gcgttgagcg ccgaagcgga
     1861 attgcagacg ttcctgctga agtatccgaa tgacaagctg actccatttg ccgagcagaa
     1921 gctgcgggat gtgcaggaag tgcttgcgga cggggaatat aaggttgcgc gattttatta
     1981 tctgcggccc gactatcaag cgtctgcggc acggctggtc gaattgaccg agcgataccc
     2041 gctttacagc gagagcgacg aggcgttgtg gatgctatcc agcatctatc agaaagccaa
     2101 ggtggcagag gcgaagaatg aagatctgag aaatcattat gccgatctcg agggtaaagt
     2161 tctcgaccgt ctcgttgcag aataccctct ttcggctcgc gcggcggcgg ccaaggaaga
     2221 attgaagggc atgggaatgg atgtgcctgc tgccgatcct gcggcagtag ctcgcatgaa
     2281 gaagcaggat gcgtacgtta aagcgcatcg cgagtccatg ttcaaagcgc cgctcgatat
     2341 catgaagtcc gggccggatt tttcaatggc tgcgcagtcg ggaatgccga acttgacgcc
     2401 tcctgcggat tcgatctcgg cgaaggatat tctcagtccg tcaaattcgg ggccgacgtt
     2461 taatttggcg gcgaatgcgg gttcgagctc aggcgggacg gacctgagcg ggagcttgcc
     2521 ggtgacggat gcgaacgatg ctgctgccgc cagtccggcg gcgagcgatg cgaacgcgaa
     2581 cgggattgta tccgcgccgg ctacatccag cgcggctgcc gatcctggga gcgctccttc
     2641 gagcaacaac cccgtaggcg aggtagacgc accgacaacg gcggcttccg atactccgtc
     2701 cgctccggcg accacggcgg cagcgagcac ggacacgccg ggtacctcga cagtagctgg
     2761 gcagagcgaa gcaaatccgc aacacgcgtc gaacacatct ccggattcgt cctcttcgtc
     2821 aaagccgaat aagaacgaat cgtcgagcaa gaaaaagaag gggttgggca agctgaatcc
     2881 cttttagcag gcttccgcgt aacaaatctt gtgcgctctc gatgctgtgc ccgccggttt
     2941 ctcgaacggc tgccagacac accacagccg ggagtggctg tgccacaaga actggccgaa
     3001 aggacagttc gcaccttaga acccctactg acaattcaag caccctcggc gattgtgttt
     3061 gttgacgccc tatgcctgtg atgctgcaat agcagtatca agacgggggc ggcgcgttgc
     3121 gcgcctactg agggttcctg tgccgaaaat tctggtcgcg gacgacaata caaatatcca
     3181 gaagatggtt tcactcgcgt tcgaagagcg cggaatcgac gttgtgtccg ttgggaacgg
     3241 cgaagcggcg gtgcggcgca tcccggacat gaacccggac ctcgtgttgg ccgatatttt
     3301 catgccagtg cgcaatgggt atgaggtttg cgagtttgta aaaaaagacg agcgattcgc
     3361 gcatgtgccc gtcattctgc tcgtgggcgc attcgatccg ctggatgaga aggaagcgcg
     3421 gagagtgggc gcagacggtg tgctgaagaa gccttttgtg ccgcccgatc cgctgatcgc
     3481 gatggtgatt tcggcgctgg agaagaatcc caagatcgcg gcggagatgg cgaaggcgcg
     3541 cgaggtggct cctcctgaga gggagccgat gcctgcgatt ctcgaagcgc cggcacgcgc
     3601 cgaggttaag ccactgccgg aatttcccga gccgagcccc gaggaagcgg cgctcgttta
     3661 tggattcggt tccggaaagc gcgggaaaga aaatcctgct gaacaagatt tggcgccggt
     3721 tgcgcctgat gctgacgaag aagaggaaac agaggaagat tttgaggcga ccgctccatc
     3781 gcacaactgg cgacgcagtg cgatggattt tgaggttcca gaggaaacct cgaagtcacc
     3841 atttgctttg gacgatgagt ctcccacggc gatgttccct tcggaaaaag atgtgccgcc
     3901 aaagcacgtt cggaagaagg aatacaggcc catggtggat actgaggtcg cgacgcgggt
     3961 cgctccgttt gcttcgctta tgccgccacc tccgcccgcg ccgcttcccg ctgcaaggct
     4021 ctcggaccag ctctctggga aagcgcccgc gtccagcgtt ccggacttcg cacctccgtc
     4081 cccggctccc gtggcacccg cgaatgaagc gcatgacagt ttcctgacgg cgacaatgct
     4141 gggaactatg ccagctccgt tttctatacc ggcggtcgaa ccgccgcaga gtgctccggt
     4201 ttcgatgtcg cctgcttcgc ccgtgccgaa ttggaaagcc ccagaatcgg agcttcccgc
     4261 tgcctcgatc tttacggaac agaacaagcc gaccgacaac ggcgaaacct caatggcacg
     4321 cgagataacc cccattacgc cggccgttga attcgggacc gcaaccagtt ttgcgacgca
     4381 gggcgcgcat tggatggatg caatgacgga ggcgctcaag gatacgcgtt cggatgccag
     4441 cgagacggcc gatcctgcgc ccgccgttaa gatggatctc gacgctccgt tgccaactac
     4501 gcctgctggc tcagtgcccg ctttagtagc agcgccagaa gccacatccc gtgcggtttc
     4561 ggaagaactg gttgtatctc cagaaccgat tcaggcagag gacgaagagt tttttgcgga
     4621 tgagaatgaa ggtgcctccg actggctgcc tgttgtctcg gcggagacga aaaaggcctc
     4681 ttccgtgacg gatgagcctg tgcttgagac gaccggccct gtgccggtac gccatttggc
     4741 ggccgatcct gaattagttg cggagccgga cgattctggt ccgatcagca aagatccggc
     4801 gctcgttgag ccgccgcccg tgcacgttac gactgagcca cttctggtag atgaagattc
     4861 ggagccagtt tcctcgcagt acgacaagca tgaagaagag acggcacccg ctttcgtctt
     4921 tgaagcgccc gcggcctcgg cagtggaaca cgttgaggcc aacgcttccg cttcggagga
     4981 accggctggt gaacgaatcc caacgatgct gccgccgaac cgcgaggttc tggcggaaat
     5041 tccctttctt tcgccgcctg cggagtttct cgctccgaag gcagaagagg caacacctgc
     5101 agtacacgac gtggacgccg tcgtgcaaaa agtactcgat aagcttgaac cgcacctacg
     5161 cgacctgcta tcccagggcg tcaagccgct ggtcgaaaac atccttcaga gcgaaaccat
     5221 gaagaagaga cggtagacag cagcatcgac ttcctctggg cttccaaaag cccctttgcg
     5281 ttcgtttgcc gtgccccgta tatttgtaag ttcacctgtc tgcaaaacca acacacatag
     5341 gaaagcatgg ccgagatacc aaaggcatac gagccacaag agatcgaaga gcgctgggcg
     5401 aaagcctggt ttgacgagaa attattccgc gcggagaatt cagcggatgg accggcgttt
     5461 tcgatcgctc tgccgccccc taatatcact ggctcgatcc acattgggca catgctggaa
     5521 catacgcaga tcgacatgtt ggtccgttgg cggcgcatgc ggggataccg cacgctgtgg
     5581 ttgcccggaa tggatcatgc gggtattggc acgcagttcg tggtggagcg cgagctagcg
     5641 aaagaaggga ttcgacgcca ggatctaggg cgcgaagaat tcgagcggcg gatttggcaa
     5701 tggaaggaaa aaagtggcgg cgcgatcaag cagcagatga ttcgcctagg cgcttcgtgc
     5761 gattggacgc gcgagaagtt tacgctggaa ccggcgcttt atcgtgcggt gatcgaagag
     5821 tttttgcgac tttaccacga cggactgatc tatcgcgcgc gctatatcgt gaattggtgc
     5881 ccacgctgcc agaccgccgt gagcgacctg gaagtcgtgc acgtggaacg gccgggacac
     5941 ctttggcatt tgcgctatcc gctggtggag acgccgaatg agtttgttgt cgtggcgact
     6001 acgcgtcccg aaacgatgct cggcgatacg gctgtggctg ttcatccgga tgacgagcgt
     6061 tataagaagc tggttggccg gaatgtgctg ctcccgctta tggatcggga aattccagtg
     6121 attgcggaca cgtatgtgga tcgcgaattc gggacgggtg tggtgaagat cacccctgcg
     6181 cacgatccga atgacttcga ggtgggcaag cgccacaacc tgccgcaaat cgacgtgatg
     6241 acgaatgacg ggcatatgag ccccgcggcc ggaaaatatg cggggctgga ccgttttgag
     6301 gcacgcaaga gagtggttga ggatctgaag gcgcttggac tcctcgagaa aatcgaggat
     6361 attacgcatt cggtgggtgt ctgcgaccgg tgcaaatcgg aaattgagcc gcgcatttcg
     6421 acgcagtggt tttgcaagat gcgtccgctg gcggagcctg cgaaaaaggc cgtggttgat
     6481 ggcgtgatcg aggtcgtgcc ggataatcag cggacgattc tgctcaactg gctggacaat
     6541 attcgcgact ggtgcatttc gcggcagctc tggtggggac atcgcattcc cgtgtggcat
     6601 tgcgacaatt gccacgagat ggtgcctgcg acggattcga gcgtggagct tttcgaaggg
     6661 catgcgcggg cagcgggcgt gccgaaaaag tgtcc
//
LOCUS       DATNDT010000226         9278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2315475, whole genome shotgun sequence.
ACCESSION   DATNDT010000226 DATNDT010000000
VERSION     DATNDT010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9278
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2315475"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..638
                     /locus_tag="VN788_07325"
     CDS             156..638
                     /locus_tag="VN788_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU20375.1"
                     /translation="MKRIFLFPVLLVGMLLGSVLSGPGAQSSASPQSLFDTVQSLDTK
                     LFDAYNHCDLTTVSSMVAEDLEFYHDVTGFSAGRQSLLDGLKENICGKVTRELVPGTL
                     EVYPIAHYGAVEIGVHRFHHPGDPTNIGEAKFITLWQNEDGVWKITRALSFDHHPFTK
                     "
     gene            complement(1071..1397)
                     /locus_tag="VN788_07330"
     CDS             complement(1071..1397)
                     /locus_tag="VN788_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20376.1"
                     /translation="MPTVKTYTLIVIYGDREIIHCDVPIVPNAYPAVGQKIEDDKYWV
                     KEILEHSETVHKILVAKVGCDWEPIDDQAGLLTVYKCKNCGRRTEVRGDFMPPVQKCR
                     KSPPEY"
     gene            2850..3194
                     /locus_tag="VN788_07335"
     CDS             2850..3194
                     /locus_tag="VN788_07335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU20377.1"
                     /translation="MSEAELKDNGSSGPTHGPMATKDSSPHFLFVAIGSHGDVLPVIT
                     LAKWLRSRNNRVTVIGPPAYSSFLEKKEIGFAALATKAGYETGMRSLGLLNHRYRMLF
                     LLRHALGGWLAH"
     gene            complement(3227..3763)
                     /locus_tag="VN788_07340"
     CDS             complement(3227..3763)
                     /locus_tag="VN788_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20378.1"
                     /translation="MLKLKQASAVLQVKPKELQNLVQFRVVKPKRFEGAYYFERSTLL
                     VAKVAFYLKESLGTSTSVLSRLMEAFSASEARLKAENPSYVVFTCRTAPDEEPIKLGV
                     PFRALEEQIGERMSRVDLYRDLPRGRKRRGWKKEFLESLSEAAKDIGEISAEEILRTV
                     HSYRRKRRTPEITIAAES"
     gene            complement(4105..5223)
                     /locus_tag="VN788_07345"
     CDS             complement(4105..5223)
                     /locus_tag="VN788_07345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU20379.1"
                     /translation="MAFRRSLLTVAGLLMLCCPAALAAPQDGAAVRVAIVGLEHGHVA
                     GFLNAFPKQQDAELVGIVDADKALCEKYEKLYHLSPSMFYPTLDDLLAHQHPQALLVY
                     TAVGEHRRVIEAGAAHGLDVMVEKPLTLSVDDALAIQRAAEAHHVEVLVNYETTWYAS
                     NRAVYDMVQQGKLGDVRRVVIHDGHQGPIEIHVHPEFLKWLTDPAENGAGALYDFGCY
                     GADLMTWLMHGEAPISVTAVSQTDKPQEYPRVDDDATIVLRYPKTQAVLMPSWNWTFN
                     RKDMEVYGTKAYAIAVNPTEVRQRFSQSAAEETVTEPPVAAPEDTSLHYLAAVVRGQI
                     LPHGDLSALDTNVTVMRILDAARESARTGKTVQLKRGQ"
     gene            5818..7629
                     /locus_tag="VN788_07350"
     CDS             5818..7629
                     /locus_tag="VN788_07350"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HXU20380.1"
                     /translation="MNQPSDPRGSNGTDRRASAAHSPRGSRSQQDTILLSDVPEAEAL
                     IASSRDTANQTAPFNPAALHLSSAAIAALGQRYDVLDAAGHGSMGNVYKARDRETGEV
                     VALKLLKPEIASDQQMMDRFKNELLFARKITHKNICRVHEFNRLGGIAYTSMEFIEGE
                     SLRSVLTRFGAMSLRKGIDLAQQMCAGLKEAHAQGIVHRDLKPENVMIDLRGNVKIMD
                     FGIARSMESLTRMTGAMVGTPAYMAPEQVAGKPVDYRTDIYSLGLILYEVFTGTQAFT
                     ADNAVAVAMKQLHESPAPPHEVEPGIPVPIERAILKCLEKGPEKRFQSIAEMEKVFSS
                     SRLSASPLAGSSTTAGRATQDAIALQPTIALPAEPVIQAAARSKPARGKSVAAVVAFA
                     MIVLFAAGVAKWVQKAQLADKLPPPTAPTAPKLPNFALTQSAPEPPPSAPTPQSDASR
                     TPLADAVSTPASAQTSSSGSNPVITKASPAKPDQSLANMAAGTAQPMGSVLRGNAPSY
                     IWVARFDREDRAQDASRKIQGLGLTSVVVPRRMEGGKRGFIVLSGPFPAVRIPSVIDW
                     LRTQGFQNVREVKIPTGQLRGRNPGLDTTYDFDGSTE"
     gene            7626..8510
                     /locus_tag="VN788_07355"
     CDS             7626..8510
                     /locus_tag="VN788_07355"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HXU20381.1"
                     /translation="MSTTAKPMIASALDFAARSDTGRARENNEDNFQVAAELGLFILS
                     DGMGGLDAGEVASRIAVETIHAHCRDAESNPDLPFEGQRIDGASETSNRLASAIRLAN
                     KLVRGAAGEAAAAHTGNGQDGSPKTAKGMGATVIALQFFADRVSVAHVGDSRAYRLRG
                     SEFLQLTDDHSLVAQQVREGHMTEAEAESSPLKNVLMRALGVDDAVETEVDEEIAMEG
                     DTYLLCSDGLTRELSDSQIAAVLGETTDAQSAADQLIDLANLAGGGDNITAIVVRVGA
                     KSGRALAKIGRWITRSGN"
     gene            8540..9277
                     /locus_tag="VN788_07360"
     CDS             8540..9277
                     /locus_tag="VN788_07360"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HXU20382.1"
                     /translation="MPKLTLKFDNAILGEVPIGSKEVSIGRSPDNALVIDNPAVSHYH
                     ARVFIEEGRMMLEDFGSMNGTFVNGQRAKMVMLKPGDSVTVGKHTIVVSDASDMDGFA
                     PVGASVARPAAPKINETVMLDTKERREFLRKVAAVGESAQVAPARVKVATLIVRSGKT
                     DRQEYALTDKLTVVGKSALATVKLKGWFAPKAAAQINRREDNSYYIGAADKTPSVNGN
                     PISHPTKLSSGDVIAVAGIEMEFLLRD"
BASE COUNT         2142 a         2647 c         2514 g         1975 t
ORIGIN      
        1 ccatgcattt ctgttcattg ttcctcatga agactccccc ggcgccaaac agggcaccga
       61 tcttacgtta gaaaaaccga ggacgaacgg aacaattcca cgcgcccggg cgtaagtgga
      121 gatgaaggcg gattaactcc atacaggagc gcaaaatgaa aaggattttt ctgttccccg
      181 tgctgttggt cgggatgttg ctcggttcgg tcttgtcggg accaggtgcg caatcctcag
      241 cctcgcctca atcgctgttc gatactgtgc aatcacttga tacgaaattg tttgacgcgt
      301 acaaccactg cgatttgacg acggtttcct ccatggtggc ggaggatctg gagttctatc
      361 acgacgtcac aggattcagt gctggccggc aatctcttct ggatggcctc aaggaaaaca
      421 tttgcggcaa agtaacgcgc gaactggttc ccggtacgtt ggaggtctat ccgatagcgc
      481 actatggtgc cgtggagatt ggcgttcatc gctttcatca tcccggcgat ccaacgaata
      541 ttggagaagc aaagttcatc actctctggc agaacgaaga tggagtgtgg aagatcactc
      601 gggcgctcag cttcgatcac catcccttca caaagtgagt cggattgtcg agtcttcaag
      661 ggcgggtggg ccacgcactc cttttcgttg tttgatggtg tggtggcaga cggcaccagt
      721 acccgcgccg gacgagttcc ttatctgaga acgaaatctg agaacgtaat ctgggaacgt
      781 attcgctcac agctcaaaat cagcgccgta ggcgcggcac aacttagccc agcgcgggag
      841 cgctgggtaa agagaagaag cgtgatgagc gccgtaggcg cgacacaagt ttctaaaagg
      901 agcggatcgg cctcacactc ccgttggttc ttgctcccag aaagtcccgc gcgccaagca
      961 ccggcgatct taggctcaga agaccaaaac gcagagtgcc gcatctcttt gcggtgcgtc
     1021 agaacagcga tagcttatcg tcgtggcgat tgtatcgttc gcccaactgc ttagtactcg
     1081 ggcggcgatt tccggcattt ttgcacgggc ggcatgaagt caccgcgtac ttccgtgcgc
     1141 ctcccgcaat tcttgcactt gtacacggta agcaaaccgg cctgatcgtc gatgggttcc
     1201 cagtcgcaac ccaccttagc cactaggatt ttgtgaaccg tttcggaatg ttccagaatc
     1261 tcttttaccc aatacttatc gtcctcgatc ttttggccga cagctgggta ggcattcggc
     1321 acaatcggca catcgcagtg gataatctcg cgatccccgt aaatcacaat tagcgtatat
     1381 gtttttactg ttggcatcgg cggtgattct actacggggt cgacatccga acaacatcct
     1441 ggcgtacttg cgaggtgact agcgaaggaa gtaaactctt ctagcgggtg gcccatgcaa
     1501 actaaccgca acaagtccgc agaagcaccg gatcgatctt acgttagaaa aatcaaaaac
     1561 gtcgggtgcc gcatctcttt gcggtgttca aagagtgcgc gccgttgagt tctcgcacgc
     1621 gccgatttga agttccgggc atcgatccgt tatctcttgc cgccgtgcac accggatctc
     1681 gctgggaaca taaggtgtgg cgacatcagc aaaacccgca ccggtcgcaa aagacgcgac
     1741 cagtgcggca cccacacgtg agccttgagc cgcagcagcg atccggtgta ggcgcgcgtc
     1801 attacacttc ggttggggct gccacccgcc gacttacggt agccataacc cctctcaatc
     1861 aaacccaatg tctgtgtaga acagcaatat ctggtgactc taatacccta tgtagtatca
     1921 gtcaagacat atttttacag tctatataag ttagtagttt acaaatgctc aaaaactgct
     1981 acaagtgaag cctcggagat attaagcgca cttctctgcg acaaatagca ccgtaagatc
     2041 accaataaaa tggtcttcga gtgttgcaat ttggcttaca ggacttccgt gcgcgcgcaa
     2101 atcgaccctg gccagaaata tcggagaggg gctggccaag gctggaaggt ccgcggaaat
     2161 tctcgatgcc gatgagctcc gatgtacaat gtgcaaagga cttggattta gcagatccga
     2221 acccgaagag agcgcacttc gtattggctg gctttgcgca ctgctcgggc gatacgatat
     2281 aaatacggtt agcgcggtca tttcaccgta ccgtgagacg cgtgcgcgtc tgaaattgct
     2341 tgttcccaat tttgttgaag tttacgttaa gacccccctt caagtatgcg tcgagcgtga
     2401 tgtaaaaggc atgtacccga aggcgatgcg aggcgaaatt cgtcaattta ctggagtcga
     2461 cgccccctat gaggaacctc tagatcccga catcgtggtg gaaaccgaca agcacgatat
     2521 tctggcgtgt actcgccaca ttctaaatca aattattgaa gtgaaccaac caaatagatg
     2581 tcagtctagg aatatcttat gcgcgaatgg aagcgagagg tgaggggtgc agtggtgttt
     2641 ggagccagct gtctggccgg aggagccctg ttcctgtggt cgagaggtct ccacggctcg
     2701 gtactggcga caaagttagc gtgggtaatt gttgaggtgg tctttgtggc ggcggcttat
     2761 tttgttaccg gcctgatttt ccaaaaatat ctgaggccaa aagacacgac aggtcgttcc
     2821 tgtcgcaagt aaagaatgaa ttgcgacgat tgtccgaggc cgaattgaaa gacaatggtt
     2881 catcaggacc gacacacggt cctatggcga cgaaagactc ctctcctcat tttctcttcg
     2941 tggcgatagg tagtcatggc gatgtgctgc ccgtcattac tctcgccaaa tggcttcgat
     3001 cgagaaacaa ccgtgtcacc gtgattggcc ctcctgcata ctcttcattc ttagaaaaga
     3061 aagagatagg tttcgctgcc ctagccacca aagccggtta tgaaaccggc atgcgtagtc
     3121 tcggattgct gaatcatcgc tatcgaatgt tatttttgtt acggcacgcc ttgggcgggt
     3181 ggctcgccca ttgattaaca cgacgaccac acgcggcttg cggcgcttag ctttccgcgg
     3241 caatggtgat ctccggggta cggcgctttc tgcggtagct gtgcaccgtg cgcaggattt
     3301 cctccgcgga gatttctccg atatccttcg ctgcctccga tagcgattcg aggaattcct
     3361 ttttccatcc ccgacgcttt ctgccgcgtg gcaggtcgcg gtatagatca acccggctca
     3421 tacgctcgcc aatctgctcc tctagcgctc gaaaagggac tccgagtttt attggttcct
     3481 catctggtgc ggtccggcaa gtgaacacaa cgtagctcgg attctcggcc ttgagccttg
     3541 cttcggacgc gctaaaagcc tccatgagcc tggagagcac gctcgtagac gttccaagag
     3601 attccttcag gtaaaaggcc acttttgcca ccagaagcgt actgcgctcg aaatagtagg
     3661 caccttcaaa tctcttcggc ttcacaacac gaaattggac gaggttttgc agctccttag
     3721 gcttaacctg gagcaccgcg ctcgcttgct tcaatttcag catttgaccg ctcatggcaa
     3781 atcccttatt atcgtctaaa ttctagtctt catttcaatc tgcgtcaagg ttagtcgcgc
     3841 tgaatggtag cgggtgcggt cgggtggtgc ttgcaaacga atcgcaacag gctcgcagaa
     3901 gcaccggatc gatccaacgc cgcgaatcaa caaacgctgg gtgccgcagc ccattgcggg
     3961 cggttagcat gggtgtgggt cttcatcgcg ccggtcgaag cgctcacaaa agtcgcggga
     4021 taacgccaaa ttagctgaac atccgaactt tgctggtttc ctttgcctcc tatacctcct
     4081 ctgcttcctc tacgtccttt tacgtcactg cccacgcttc aactgcaccg tctttccagt
     4141 tcgcgccgat tcacgcgcgg catccagaat ccgcatcaca gtcacattcg tatcaagggc
     4201 gctcaaatcg ccgtgcggca gaatctgccc gcgcacaacg gcagccagat agtgcaaaga
     4261 agtgtcttcc ggtgcagcaa ccggcggttc agtcaccgtc tcctcggccg cggactggct
     4321 gaagcgctgt cttacttccg taggattcac ggcaattgcg tacgcttttg ttccataaac
     4381 ttccatgtct ttacgattga aggtccagtt ccacgaaggc atcagcacgg cctgcgtctt
     4441 cggatagcgc aaaacgatgg tcgcgtcgtc atcgacccgc ggatactctt gcggcttgtc
     4501 cgtttgcgag acggccgtca cgctgattgg tgcctcgcca tgcataagcc aggtcatcag
     4561 atcggcgcca taacagccga aatcgtagag cgcgcccgcg ccgttctcgg ctggatcggt
     4621 gagccactta agaaattccg gatgcacatg aatctcaatc ggtccctgat gcccatcatg
     4681 aatcacgact cgacgaacgt cgccgagctt gccctgctga accatgtcat aaaccgcgcg
     4741 attgctcgca taccatgtcg tttcgtaatt cacgagcacc tcgacatgat gtgcttccgc
     4801 tgcccgttgg atcgcaagcg cgtcgtccac cgaaagcgtg agcggtttct ccaccatcac
     4861 atcgagtcca tgcgccgctc ccgcctcaat cacgcgtcgg tgctcgccca ctgccgtgta
     4921 aaccagcaat gcctgaggat gctggtgcgc aagcaaatcg tccagcgtcg gataaaacat
     4981 gctcgggctg aggtgataca gcttctcgta tttctcgcag agcgccttgt cggcgtccac
     5041 gatgccgacc aactcggcat cctgttgctt gggaaatgcg ttcagaaatc cggcaacgtg
     5101 cccgtgctcc agtccaacga tcgccacacg caccgccgcg ccatcctgcg gagcggcaag
     5161 cgccgcgggg caacacaaca tcaagaggcc ggcaacggtg agaagggaac gacgaaatgc
     5221 catgggatgc ctcctttctt gtccaacgag gccctatgct aacgcagcct ggattgaata
     5281 caacgccttt gctcgctgcc caaattacag ccaacttgcc actacggctg ctcacgggcc
     5341 tcaggtcatg gccgcgagtc aagaattacg aatcacggcc ctcagtacta tgtccgtatt
     5401 tcccacaatt gaaatcgttt tttgcgcgtg ctaccttttc gtgttttttg aagatgggga
     5461 cctgaaaaac ggagaaaaat cggggaggaa aggggagtcg cgatcgccgt cacgaatcac
     5521 gagtcataag tcgcgagcgg ttgcctttcc gccagtcacc attcacaatc acgcttttct
     5581 aatcgccggt ctgccgatta gaaatcagcg taagtccagc gccatcaacg aaaacggaaa
     5641 ttctaatcga caaaaaagag gcatttttta gctcaaaaat ggcggtctct aacggccaca
     5701 aaatccggga agttggcgcc aaccgtcgcc gaatcgtacc agatactctt tccttacagg
     5761 acccttgcgc cgccaggctt cgaccggatt tctcctttat cctagtacga cagcgccatg
     5821 aaccagccat ccgatccccg tggctccaac gggaccgacc gccgtgcatc tgcggcccat
     5881 tcgccgcgag gctcgcgctc gcagcaggac accatcctgc tcagcgacgt tcccgaagcc
     5941 gaagcgctga tcgcttcatc ccgggacact gcgaatcaga ccgcgccgtt caacccggcg
     6001 gctctacatc tcagttccgc cgcaatagct gcccttggcc agcgctacga cgttctcgac
     6061 gcggctggac acggcagcat gggcaatgtt tacaaggctc gcgaccgcga aacgggcgaa
     6121 gtcgtcgcct tgaagctcct gaagccggaa atcgcctccg atcagcagat gatggaccgc
     6181 ttcaaaaacg agcttctctt cgcccgcaaa atcactcaca aaaatatctg ccgcgtccac
     6241 gagttcaacc gcctcggcgg catcgcatac acctcgatgg aattcatcga aggcgagagc
     6301 ctccgctcgg tcctgactcg cttcggcgct atgtccttgc gaaagggaat cgacctcgca
     6361 cagcaaatgt gcgccggcct caaagaggct catgcacagg gcatcgttca tcgcgatctg
     6421 aaacccgaaa acgtgatgat cgatttacgc ggcaacgtaa agatcatgga cttcggcatc
     6481 gcgcgttcca tggagtcgct cacgcgcatg acaggcgcga tggtcggcac tccggcctac
     6541 atggcgcccg aacaggtcgc aggcaagccc gtggattatc gcaccgacat ttactccctt
     6601 ggcctgatcc tctacgaagt cttcacgggc acccaggcgt tcaccgccga caatgccgtc
     6661 gccgtggcga tgaagcaatt gcacgagtcg cccgcgccgc cgcacgaggt cgaacccggc
     6721 atcccggttc caatcgaacg cgccattcta aaatgtctcg aaaaggggcc cgagaagcgc
     6781 ttccagtcca tcgccgaaat ggaaaaagtt ttctcctctt cgcgtctgtc ggcgtctccg
     6841 ctagcgggaa gcagcactac cgcaggtcgc gccacacagg atgctatcgc ccttcagccg
     6901 acaatcgcgc tgccggccga gccagtgatc caggcagcgg cacggtcgaa gccagcccga
     6961 ggaaaatcgg tcgccgccgt cgtcgcgttc gcgatgatcg tgctctttgc ggcaggcgtg
     7021 gcaaaatggg tgcaaaaagc gcaattagcg gacaaattgc cgccacctac ggcgccaact
     7081 gcgccaaaat tgcccaattt tgccctaacg caatccgctc ccgaaccgcc accgtcggct
     7141 cccacaccgc agtccgatgc ctctcgtacg ccgttagcgg acgctgtctc caccccagcc
     7201 agcgcccaga catcgtcctc tggctcgaat cccgtaatca caaaggcgtc accagcaaag
     7261 cccgatcaga gcttggcgaa catggcagcc ggcacagccc agccgatggg atcagtcctt
     7321 cgcggtaacg ccccatctta catatgggta gcgcgattcg accgcgaaga tcgcgcgcaa
     7381 gacgcatcgc gcaaaataca gggcctcgga ctcacgtccg ttgttgtccc acgacggatg
     7441 gagggaggca aaagaggttt cattgtgtta tccgggccat ttccagcggt gcgcatccca
     7501 agcgtgatcg attggctccg aacgcaaggt tttcaaaacg tccgcgaagt gaaaatccca
     7561 accggccagt tgcgcggccg caatccaggg ttggatacga catacgattt cgacgggagc
     7621 accgagtgag cactacagcc aaaccgatga ttgcatcggc gctggatttc gccgcgcgct
     7681 cggatacggg gcgcgcccgc gaaaataacg aggacaattt ccaagttgct gcggaactcg
     7741 ggctctttat tctttccgac gggatgggcg gactggacgc gggtgaagtc gcaagcagaa
     7801 ttgcggtgga aacaattcac gcgcactgcc gcgatgcaga atcgaatccg gatttgcctt
     7861 tcgaagggca acgcatcgat ggcgcttcgg aaacctccaa tcggttagcg agtgcgattc
     7921 ggctggcaaa caaattggtt cgaggagcgg caggtgaagc agcagccgca cataccggca
     7981 acggacagga tggttctcca aaaactgcaa aagggatggg cgccacggtt attgcactgc
     8041 aattctttgc cgatcgcgtg agcgtggctc acgttggtga cagccgggcg tatcggcttc
     8101 gcggcagtga gtttttgcaa ctcacagacg atcactcgct cgtcgcacag caggttcgtg
     8161 aggggcacat gaccgaagcc gaggccgaat cgagtccgct gaaaaacgtg ctgatgcgcg
     8221 ccctcggagt cgacgacgcg gttgaaaccg aagtcgacga ggaaatcgcg atggaaggcg
     8281 acacatacct cctctgttcc gacggattaa cgcgcgaact ctcggacagc cagatcgccg
     8341 cagttttagg ggaaacgacg gatgcgcaaa gcgcagccga ccagttgatc gatctcgcaa
     8401 atctggcggg cgggggagac aacatcactg caatagttgt gcgagttggt gcaaagtccg
     8461 gcagggcact cgcaaaaatc gggcgctgga tcacacgttc tggaaactga cgagatcgaa
     8521 aaggcagaag gagaaactga tgccaaagct gacactcaag tttgacaacg caatcctggg
     8581 ggaagttcct atcggctcga aagaggtaag catcgggcgt tcgccggaca atgcgctcgt
     8641 aatcgataat ccggctgtgt cgcactatca cgcgcgagtg tttattgagg aaggccgcat
     8701 gatgctcgaa gacttcggca gcatgaacgg cacattcgtc aacggccagc gcgcgaagat
     8761 ggtgatgcta aagccgggcg actccgtaac cgtgggcaag cacactatcg ttgtctccga
     8821 tgcaagtgac atggacgggt tcgcgccggt aggtgctagc gttgcgaggc cggcggcgcc
     8881 aaagatcaat gaaaccgtga tgctcgacac taaagagcgg cgcgaatttt tgcgaaaagt
     8941 ggcggccgtg ggcgagagcg cgcaggtggc tccggcacgt gtgaaagtgg ccacgttgat
     9001 cgtacgcagt ggaaaaacag accgccagga atatgccctc acggacaaac tgaccgtcgt
     9061 cggcaagtca gcactggcaa ccgtgaaact gaagggttgg ttcgcaccga aagcagcggc
     9121 gcaaatcaat cggcgagagg acaattccta ttacatcggt gcagcagata agacgcccag
     9181 tgtgaacggc aatcccattt cgcatccgac gaaactatcc tccggcgacg taatcgccgt
     9241 ggcaggtatc gaaatggaat ttctgcttcg cgattagt
//
LOCUS       DATNDT010000227         3531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1738078, whole genome shotgun sequence.
ACCESSION   DATNDT010000227 DATNDT010000000
VERSION     DATNDT010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3531
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1738078"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(381..1481)
                     /locus_tag="VN788_07365"
     CDS             complement(381..1481)
                     /locus_tag="VN788_07365"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20383.1"
                     /translation="MIGHMILRSALHRPVRTIITVIAVAVEVTLVIIVVGLTSGLLTE
                     SAKRTEGIGADIMVQPPSASIFMAFSGAPMPIAIADKLRQLEYVQAVAPVLVQFNSVN
                     GFDLVYGIQSDTFRAVSGGFTLYSGTLLQNPNDILVDDIYARAKKLKVGDTVRILEHD
                     FHLAGIVEHGKGARLFVRMSTLQEMSGAHEKASAFFIKCDRSDHTPAVTDEIRGLLPR
                     YEIRPLKDFFSQMTSSNLPGLAAFINVMIAIAVCIGFLVTFLSMYTTIVERTREIGVL
                     KSLGASKSYIAEAILSETTLMCLAGVVGGIALSFLVRYIILRAFPSLTILITLKWILL
                     AGMIAVSGGLLGASYPAWLASRKDPVEALAYE"
     gene            complement(1478..2440)
                     /locus_tag="VN788_07370"
     CDS             complement(1478..2440)
                     /locus_tag="VN788_07370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20384.1"
                     /translation="MLTTRTYRARLRVLWLVSITSWLLFSGCAMNQPVAKKPTAAPVP
                     LQTATREDLIAQYNRQANGVTSINASVTMTLTAGSAYSGVIKQYHEVSGFILAEKPAK
                     IRVIGQAPVVGTNIFDMESDGQTFNIFIPSQNKFLTGPANLERPSAKPIENLRPQHLT
                     SAIFWQPIPDSAPVLIENASEAGGQFYVLTVIRIAGGARGAPKEASPRTNWEIARKIW
                     FDRTNLSISRLQTYDAGGKIASDIRYTRWDAFDSVKYPRQIGLSRPANDYALQIGITK
                     ATFNEPLPDDRFVLRKPPGAELVNVGESTPATKIPDGSSGEKKP"
     gene            complement(2592..3203)
                     /locus_tag="VN788_07375"
     CDS             complement(2592..3203)
                     /locus_tag="VN788_07375"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004889507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HXU20385.1"
                     /translation="MSHTLPPLPYSFDALEPYIDAKTMEIHHDKHHVAYVTNLNKALE
                     GHADLQRLSVEELLAQIGKVPENIRTAVRNNGGGHMNHSMFWKIMKKGGGGEPSGELA
                     DAIKKTFGTFAEFKTKFNQAGTTRFGSGWAWLLFRDGKLAIESTPNQDNPILDGGKAF
                     FGLDVWEHAYYLKYQNRRPEYIEAWWNVINWDQVTQNYTAAKK"
     gene            3346..>3531
                     /locus_tag="VN788_07380"
     CDS             3346..>3531
                     /locus_tag="VN788_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXU20386.1"
                     /translation="MPSVEHVEKHFTATAAVRDIVIGMSDGLTVPFALAAGLTGAAAA
                     LSHGNTIIVTAGFAEIAA"
BASE COUNT          773 a          973 c          961 g          824 t
ORIGIN      
        1 gtcgccattg gcgtgatcct gcacgatcgc cggtatcaac ccattttcct tttcgaaatc
       61 gagttccatt tgctctcttt tagcctttct gaccgtccgc cttgtgccgc ggccctgtag
      121 tgaccgcatt tcatccccaa aaacaaaaag cccgcaggcg ctttccgcgc cagcgggccg
      181 atgagtgcag ctattttcgt tccagctagc cacaccgccg cgggcacggt ttcacgtgat
      241 gatgatggct atggtgcgcc cgcagcagtt tcatgacgat ttgaaattaa gatacctcgc
      301 cgcctttgtc aagcgcggag tcgcttaaat gtagaaaaat gacggcacct gcaattgtaa
      361 gttgcggaat gcaagagcgt ttactcgtac gcgagagctt caacaggatc cttgcggctg
      421 gcgagccacg ccgggtagga agcgccaagc aggccaccag aaactgcgat catgcccgcc
      481 agcaaaatcc acttcagcgt gatcagaatg gtaagcgaag ggaaagcccg caggatgatg
      541 taacgcacca agaagctgag ggcgatcccg ccgacgacgc cggccaggca catcagggtc
      601 gtctcgctga gaatcgcttc ggcaatataa ctcttcgagg ctcccagcga ttttaggacc
      661 ccaatctcgc gagtcctttc cacgatggtt gtatacattg agaggaaagt gaccaggaat
      721 ccgatacaga cggctatggc aatcattaca ttgataaacg cggcgagacc gggaaggttg
      781 gatgaggtca tctgtgaaaa gaaatccttg agcggacgta tctcgtatcg aggcagcaga
      841 ccccggattt catccgtgac ggcgggagtg tggtccgacc gatcgcattt gatgaagaag
      901 gccgatgcct tttcatgcgc gcccgacatt tcttgcaggg tggacatccg gacgaataac
      961 cgcgcgccct tgccgtgctc cacgatgccc gctaaatgaa aatcatgttc gagtattcga
     1021 actgtgtcgc caacctttaa tttttttgcc cgcgcgtaaa tgtcgtccac gagtatgtcg
     1081 ttcggatttt gcaagagcgt gcccgagtac agcgtgaacc ccccggagac agcgcgaaaa
     1141 gtatcggact ggatgccgta aaccagatcg aagccattca cggaattgaa ttgcaccaga
     1201 acaggggcca cggcttgcac gtattcgagc tgccgcaatt tgtcggcgat ggcgataggc
     1261 attggagcgc cgctaaaagc catgaagatg gaagcggatg gaggctggac catgatatcc
     1321 gcgccgattc cttcggtgcg cttcgcggac tcggtgagca ggccgctcgt aagtcccacg
     1381 acgatgatta ccagcgttac ttccaccgcc actgcaatca cggtgataat cgtacgaacg
     1441 ggccggtgca gagcactccg caagatcatg tgcccgatca cggcttcttt tctcctgaag
     1501 atccatccgg tatcttggtt gccggggtac tttcgccgac gttcacaagt tccgctcctg
     1561 gcggcttgcg cagcacaaac cgatcatccg gcagcggttc gttgaatgtc gccttcgtga
     1621 tgccgatctg tagggcgtag tcgttggccg gtctgctaag ccctatttga cgaggatatt
     1681 tcaccgaatc aaacgcatcc cagcgagtat aacgaatgtc agaagctatc ttcccacccg
     1741 catcgtaagt ttgaaggcgg gagatgctca ggttggtacg gtcgaaccaa attttgcgcg
     1801 ctatttccca gttggttctg ggggaagctt cctttggtgc gcccctggcc ccgcctgcga
     1861 ttcggatgac cgtcagcaca taaaattgac caccagcttc acttgcattt tcgatcagca
     1921 caggcgcgct atccggaatc ggctgccaaa aaatcgcgct ggtgagatgt tgcgggcgga
     1981 ggttctcaat gggttttgcc gaaggccgct ccaaatttgc cggtcccgtc aagaatttat
     2041 tctgagacgg aatgaaaatg ttgaacgtct gcccatcgct ttccatgtcg aaaatattcg
     2101 tgcccacaac gggagcctgc ccgatcacgc gtattttagc gggcttttcc gcaagaatga
     2161 atccgctcac ctcgtgatat tgcttgatta cgccgctgta ggccgaacct gcagtcagag
     2221 tcatcgtcac cgaagcgttg atcgatgtca ccccgttcgc ctgacggttg tattgagcga
     2281 ttaaatcttc tcgagtggcc gtttggaggg gaaccggggc ggccgtgggt tttttcgcca
     2341 ccggctggtt catcgcgcag ccagagaaaa gcagccagga agtgatggag acaagccaaa
     2401 ggacgcgaag ccgcgcccga tacgttcgcg tggtgagcac tacatgcaaa cttatcagtc
     2461 ggcttgggga gtgtcaacga agcgcagcga agctcgcttc attggatacg cggaatccgt
     2521 ttcgaggaaa atgaggagct tcaactgaag tctgaagctc ctcgaatggg tggcctttcc
     2581 tcgccgctgt tttacttctt cgcagcagtg tagttttggg tgacctggtc ccaattaatc
     2641 acgttccacc aggcctcgat gtattccggc cggcggtttt gatatttcag atagtaagcg
     2701 tgctcccaaa cgtccaggcc aaagaaagcc ttaccaccgt ccagtattgg attgtcctga
     2761 ttgggcgtcg actcgatggc cagcttgccg tctcggaaca acagccaggc ccatcccgaa
     2821 ccaaatcgcg tcgtccctgc ctgattgaat tttgtcttga attcagcaaa cgtgccgaat
     2881 gttttcttga tggcatcggc aagttcgccc gaaggctccc ctcctccgcc ctttttcata
     2941 atcttccaga acatcgaatg attcatgtgg ccgccgccat tattgcgtac tgcggtgcgg
     3001 atattctcag gaaccttgcc gatctgcgcg agcagttcct ccacgctgag ccgttggagg
     3061 tcggcatggc cttcgagagc tttattcagg tttgtgacat atgcgacatg atgtttgtcg
     3121 tgatggattt ccattgtctt cgcgtcaatg taaggctcga gcgcgtcgaa ggagtacggc
     3181 agtggtggca aagtgtggct cattttggga gcggctcctt gtgcggaagt cgaactcatt
     3241 atctacctcc tgaatttcag gttcctacgc cataattata cgcgccttgc gggtaagggg
     3301 tggaacaagc gccttggatt aatcaaatac cttacaggag gcttcatgcc gtccgtcgag
     3361 cacgtagaaa agcactttac cgccacggcc gccgtgcggg acatcgtgat cggaatgtcc
     3421 gacggtctaa ccgtaccctt cgccctcgcc gcagggctca ctggcgcagc tgcggctctc
     3481 tcccacggca acaccatcat cgttaccgcc ggcttcgccg aaatagccgc t
//
LOCUS       DATNDT010000228         3103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3470991, whole genome shotgun sequence.
ACCESSION   DATNDT010000228 DATNDT010000000
VERSION     DATNDT010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3470991"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="VN788_07385"
     CDS             <1..833
                     /locus_tag="VN788_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HXU20387.1"
                     /translation="GYQIDDVILIEHDLLAEYTPDGYCIALRRAIENAKPDLVLLPHT
                     YQARDFAPKLAASMQKGMIGDCIAYRHENAKLIFVRQMFQGRTAADVVFTGEAPWIAS
                     FQAGAFRGDTAAKRSAGKAEVKAASVELKPEQIRTKPLELFREAKQAVDLTQAPILVS
                     VGRGIKAPDNIPMAEKLAKLLGGEISASRPICDEGWLPMDRQIGSSGQTVVPKLYLAL
                     GISGAIQHVVGMKGSRTIVAINKDQNAPIFEIADYGIVGDLFEIVPALIEELEKAPAS
                     "
     gene            complement(876..1124)
                     /locus_tag="VN788_07390"
     CDS             complement(876..1124)
                     /locus_tag="VN788_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20388.1"
                     /translation="MSEPTKEELLARIAELEKQTGSRPKGSLEFRVGEKGGVSVYGLG
                     RFPVTLYYEQWIRLLDATDNLRSFLEENKSRLKLKNRD"
     gene            complement(1455..2444)
                     /locus_tag="VN788_07395"
     CDS             complement(1455..2444)
                     /locus_tag="VN788_07395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXU20389.1"
                     /translation="MTQNSKVRNQVLIADDDPLSRRMLEAFLAKSGYRVVTAVDGVDA
                     LRILSAEDSPPLAILDWMMPGLEGPQVCERVRALPERAYVYILLLTARNDKQDLLNGL
                     QSGADDYLTKPFDAQELRARLHVGQRILDLQDSLIAAREEMRFHATHDLLTGIDNRSV
                     AIDAMNRESSRQARDGGSFGVVLLDLDHFKNVNDTLGHLAGDAVLKESARRMKLAVRP
                     YDTVGRYGGEEFLVVAPSSNGDGTLVLAERIREAIQSTLVTTQAGEVRVTASCGVAAS
                     TESNRLDTHTLLRLADEALYRAKQRGRNRSELAAYESAIAPALAPDAAPVKSL"
     gene            complement(2479..>3103)
                     /locus_tag="VN788_07400"
     CDS             complement(2479..>3103)
                     /locus_tag="VN788_07400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020475639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU20390.1"
                     /translation="LMDVQMPVLDGLDAIRAIRAKEQSSGSHLPIIALTAHAMKGDRE
                     RCLASGADDYVTKPIRTPDLLAAIDRATSNNTSPANGAPPVPASHSDIRPVLDVAAAL
                     ERVEDDRELLENLMGIFAGECSQNITELRKSLEARDLVLLERLAHTMKGAAASLAAGP
                     VAEAAFALEKQARTGEVGDAELLLESLIGEMNRLLPEIELFCRKVAP"
BASE COUNT          637 a          860 c          938 g          668 t
ORIGIN      
        1 ctggttatca gatagatgat gtaatcctga ttgagcacga tctgctggct gaatacacgc
       61 cggacggtta ttgcatcgcg ctgcgccggg cgatcgaaaa cgcgaagcct gatctcgttt
      121 tacttccgca tacttatcaa gcgcgcgatt tcgcgccgaa gctggcggcg tcgatgcaaa
      181 agggaatgat cggggattgc attgcctacc gccatgaaaa cgcgaagctg atttttgtgc
      241 gccagatgtt tcaggggcgg acggcggcgg atgtggtgtt caccggcgag gcgccatgga
      301 tcgcgtcgtt tcaggccggc gcatttcggg gagacacggc ggcgaagcgg tccgccggca
      361 aagcggaggt gaaggctgcg agcgtcgaat tgaaaccgga gcagattcgc accaagccgc
      421 tggaactttt ccgcgaagcg aaacaagctg tggacctgac gcaggcgccc attttggttt
      481 ctgttggacg cggaataaag gcgcccgaca atattcccat ggcggaaaag ttagcgaaac
      541 ttttgggcgg agagatttcc gcttcgcgcc ctatttgcga tgaaggatgg ctgccgatgg
      601 accggcaaat cggaagctcg ggacagaccg tggtgcccaa gctctatctc gctctgggaa
      661 tcagcggggc gattcagcat gtggtgggaa tgaaaggctc gcggacgatt gtagccatca
      721 acaaggatca gaatgcgccg attttcgaaa tcgctgatta tgggatcgta ggcgacctct
      781 ttgaaattgt tcccgcgctg attgaggaat tggaaaaggc gccggcgagt tgaggccttg
      841 aggttcagca acgcggaggc cggtgcttgc tcgagttaat ctctattttt tagcttcagg
      901 cggcttttat tttcttctag aaaagaccgg agattgtcgg tcgcgtcaag caggcgaatc
      961 cattgttcgt agtagagagt gacgggaaag cgcccgaggc cgtagacgct cacacctccc
     1021 ttttcaccga cgcggaattc gagacttccc tttggtctgg aaccagtctg cttttcgagt
     1081 tctgcgatgc gggccagcaa ttcttctttg gttggttccg acattttgac actctcctaa
     1141 atcggaatca tagcaggtcg aggacgaggc tgcgagcggg ccaggatgtt gtgtgggcgg
     1201 aatcccagga tacaaagaaa attttcacat gaggggtgga aatcgcgcaa gtggttgttg
     1261 gtgtaaagaa aagtcgtcca cggtgcgtgt ggactttcgg cgctttttac accgaggggc
     1321 gcgacgaacg cactgacgaa attcacatcg aagagaggtc cttcgactgt gcgtcccggc
     1381 gcttcgcaca gaaccaaagg cgcgggacgc tccgctccct tccttcgtca gggcaagcag
     1441 gatgacggcg tatattagag agactttacc ggcgccgcgt ccggtgccag agcaggcgcg
     1501 atcgcagatt catatgcggc aagttccgag cgattgcgtc cacgctgctt tgcgcgatac
     1561 aacgcttcgt cggctagacg cagcaaggtg tgggtatcga ggcgattgct ctcggtactg
     1621 gccgccacgc cgcagctggc ggtcacgcgc acctcgccag cctgggtggt caccagcgtg
     1681 gattgaatcg cttcgcggat tctctccgcc aagaccaaag ttccgtcccc attcgacgag
     1741 ggtgcgacga cgagaaactc ctcgccgccg tagcgcccca cggtatcgta gggacgaacg
     1801 gctagcttca tccgccgcgc cgactctttt agcaccgcat ccccggcgag atggcccaag
     1861 gtgtcgttga cgtttttgaa gtgatccagg tccagcagaa caactccgaa cgatcctccg
     1921 tcacgcgctt gccgcgatga ctcccggttc atggcatcga ttgccactga gcgattgtcg
     1981 atgcccgtca gcaagtcatg agtggcatgg aagcgcattt cttctcgcgc ggcaatcagg
     2041 ctatcctgca gatcgaggat gcgctgccca acgtgcaggc gcgcgcgaag ctcctgtgcg
     2101 tcaaatggtt tggtgagata gtcgtccgcc ccggactgga ggccgttcag caagtcctgt
     2161 ttgtcgttgc gagccgtaag caacaggatg tagacgtaag cccgctccgg cagcgcccgc
     2221 acacgctcgc aaacctgagg tccttccaaa cccggcatca tccagtccaa aatcgcgagc
     2281 ggcggggaat cttcggcgga aagaatcctc agggcatcta cgccatccac ggcggttaca
     2341 acgcggtatc cggacttggc aaggaacgcc tccagcatgc ggcgagatag aggatcgtcg
     2401 tccgcgatca gaacttgatt tctgaccttg ctgttctggg tcatgctgcg atttatccgg
     2461 cggtccgagc tggcgtgctc agggcgccac cttccggcaa aacaattcga tctccggcaa
     2521 caggcgattc atttcgccga tcaagctttc gagcaacagt tccgcatctc ccacctcgcc
     2581 ggtacgtgct tgcttttcca acgcgaatgc tgcttcggca accggacctg cagcgagact
     2641 tgcggctgct cccttcatcg tatgcgccaa gcgttcgagc agcaccagat cgcgggcctc
     2701 aagcgatttg cggagttcag ttatgttctg ggaacactcg ccggcgaata ttcccatgag
     2761 attttccaga agctcgcggt catcttccac acgctctaat gccgctgcga cgtccaacac
     2821 gggacggatg tcggaatgcg acgcgggaac cggcggagct ccgttagcag ggctggtgtt
     2881 gttgctcgtc gcgcggtcga ttgccgcgag gaggtcaggg gttcgaatgg gtttggtcac
     2941 gtaatcatcg gctcctgatg ccaggcaccg ctcgcgatct cccttcatgg cgtgtgccgt
     3001 cagagctatg atcggcaaat gtgagccgga actttgctct ttcgcgcgga tggcgcgaat
     3061 agcgtcaagc ccatcgagca ccggcatctg cacgtccatc agg
//
LOCUS       DATNDT010000229         5836 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2888406, whole genome shotgun sequence.
ACCESSION   DATNDT010000229 DATNDT010000000
VERSION     DATNDT010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2888406"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..650
                     /locus_tag="VN788_07405"
     CDS             135..650
                     /locus_tag="VN788_07405"
                     /inference="COORDINATES: protein motif:HMM:NF025046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4149 domain-containing protein"
                     /protein_id="HXU20391.1"
                     /translation="MSTLWRFLKVFTLGTWVGSMIFFGMVAGVLFTTLKSTDDAGNIV
                     AILILQLHVLGIIAALIYLVVALASARSWKGLVQPASILVELMLLVTLVSQLWITPTM
                     DHLRQQMGSYASTAANNPLRVQFDHLHVISVRLEGGVLILGVIALFLTVWQRFSSPLP
                     PAAAMPATANK"
     gene            complement(665..1438)
                     /locus_tag="VN788_07410"
     CDS             complement(665..1438)
                     /locus_tag="VN788_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HXU20392.1"
                     /translation="MNATATKLLGSVDVAPAAKLWAFDHKAAIEHLTKNDRRLSQLIS
                     KCVEFRLEIEEAQSPYEALLEAIAYQSISGKAAATIFARIKALGANGRCPTPEEILRV
                     RKPALRKAGLSGAKILAAKDLARKTIEGVVPTLEDAQRMSDHELVDRLISVRGIGAWT
                     VEMFLIFRLGRPDVLPIHDYGVQKGFALTYGKKDIPKPRELAKFGERWRPYRTVASWY
                     MWRAVQLAGKDARKVTKTTTALRKRAAKAKASRPKSARR"
     gene            complement(1874..2143)
                     /locus_tag="VN788_07415"
     CDS             complement(1874..2143)
                     /locus_tag="VN788_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016886866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HXU20393.1"
                     /translation="MTKKAISAGAVHSVPADLRKVLISDPAARAKWEDITPLARNEWI
                     CWVISVKKPETRRQHVERVRTELKEGMRRPCCWPGCPHRQKTGTP"
     gene            complement(2433..2966)
                     /locus_tag="VN788_07420"
     CDS             complement(2433..2966)
                     /locus_tag="VN788_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20394.1"
                     /translation="MFEDYLQDSHHFLLAAQKLSKQHNDRQARRLYRASVFYASGAIE
                     SFINYIGDSFAKGNSIPKDEIRFLNDKTLKFDAAKGFVEKTEYHRPDDKIRCVLRRFT
                     PSFDFRSPTWSTFMEFKRFRDALVHPRALDDDIELSQYSKEVTRGLASIIDIMNDIST
                     GVWQRPLRKQLLDLIPD"
     gene            complement(2970..4223)
                     /locus_tag="VN788_07425"
     CDS             complement(2970..4223)
                     /locus_tag="VN788_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20395.1"
                     /translation="MSEPLVLPSRPHPPAPVTKKVAEFLEKFPPHEVSSGGKALLFLD
                     EKSGAYYLSCHLDGKVLAGACDSEASLDGDEEDEIYKLNREITEDEAAYKLMEQDAIK
                     GRSFEDMVLEYDTKYRAKKPLKVYGGQHRLRAITKALPAKGATLHGVRIYFELSRDQK
                     VEIATINNTAIAVPNDLLDRMKEQLLGTDLRDWCQRVGLLEKSTDFSDRRSPDVPTVR
                     AARTLILNFCLGKDVKDVGDFHQPILCKSGGIDEEYMKIRSGIAWSEPALEQMGKQFA
                     RLHRLQRKTVSDRDEDDNAEFARKALSLSVVASWGYAAGLFQRDPALLKNLYSIPDNV
                     SPPDDPLNAKALSQARLKGTDPDTYRGLGTRSSPKELGRMLEVFWVLANKGKPRITKE
                     LANAAIQSYEAKKASHDAKTVLGKL"
     gene            complement(4264..4401)
                     /locus_tag="VN788_07430"
     CDS             complement(4264..4401)
                     /locus_tag="VN788_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20396.1"
                     /translation="MNQLYYGDNLDVLRRYIKDESVDLVYLDPPPKTDAMVPRRTCTV
                     R"
     gene            5157..>5836
                     /locus_tag="VN788_07435"
     CDS             5157..>5836
                     /locus_tag="VN788_07435"
                     /inference="COORDINATES: protein motif:HMM:NF014036.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HXU20397.1"
                     /translation="MQTGIIGLPQVGKTSLFRILTGVRVDAKTQPGQMHVGIARVPDA
                     RIVKLAELFKPKKITYATVEYVDIGGLQKDRAKDSASLVPLREADALMHVVRLFEDPS
                     MPHPAGSLDALRDIESVEIELMLNDLEQATKRLERVEKDLKKKKDPLVEAEKNVLDRC
                     IKALESETPLRELDFKPDELKIVNGFMFVSRKPMLFALNLGDDEAADMQGAVARHNLS
                     KLAGKTRTA"
BASE COUNT         1221 a         1539 c         1673 g         1403 t
ORIGIN      
        1 gagaggtcca aggttcaagt ccttgatcgc ccaccatttc aaattccgct gcaaatccaa
       61 aatgctttga gattgcttcg cgcggagcgt attatcacgc ccgagaacga aactccattt
      121 tagcggaaaa gacgatgagc acgctctggc gtttcctgaa ggttttcacg ttaggcacct
      181 gggtcggcag catgattttc ttcggcatgg tggccggagt gctgttcacc acgctaaaga
      241 gcaccgatga cgccggaaat atcgtcgcca tactgatttt gcagctgcac gtcctgggaa
      301 ttattgcggc gctgatttat ctggtggtgg cgctggcctc ggcgcgctcg tggaagggat
      361 tggtgcagcc tgcgtcaatt ttagtggaac tgatgctgct cgtgacgctc gtttcgcagc
      421 tatggattac gccgacgatg gatcatttgc gccagcaaat gggttcatac gcaagcacgg
      481 cggcaaataa tccgctacgc gtgcaattcg accatctcca cgtgatttcc gtgcgcctcg
      541 agggcggcgt gttaatcctc ggagtaatcg cgctgtttct caccgtgtgg cagcgattct
      601 cttcgccgct tccgcccgct gctgcaatgc cagccacggc aaataaatag tcgattccta
      661 cgattcaccg gcgtgctgac ttcgggcgcg acgctttcgc tttggcggcg cgttttcgca
      721 gcgccgtggt tgtcttcgtc actttgcgcg catcttttcc cgccagttgt accgctctcc
      781 acatgtacca cgaagcgacc gtgcgatagg gccgccagcg ctcgccgaat ttcgcgagtt
      841 cgcgcggctt gggaatgtct ttcttgccgt acgtcagcgc aaatcccttt tgcacgccgt
      901 aatcgtggat gggcagcacg tcgggacggc cgaggcggaa aatcagaaac atctcgacgg
      961 tccacgcgcc aatgccgcga acggagatca ggcggtccac caactcatgg tcggacattc
     1021 tttgcgcgtc ctcgagcgtc ggcaccacgc cttcgatggt tttccgcgcc aagtctttcg
     1081 ccgctagaat tttcgcgcca gagagccccg ctttgcgcag cgccggtttg cgcacgcgca
     1141 ggatttcctc gggtgtaggg cagcgcccgt tcgcgcccag cgccttgatg cgcgcaaaaa
     1201 tcgtcgccgc ggctttgccg gagatgctct ggtacgcgat ggcttcgagc agcgcttcgt
     1261 aggggctttg cgcctcttcg atttccaggc gaaattcgac gcattttgag atgagctgcg
     1321 acaggcggcg atcgttttta gtcagatgct cgatggccgc cttgtggtcg aacgcccaca
     1381 gcttcgcggc gggagccacg tctaccgaac ccaggagctt tgttgcggtc gcattcatcg
     1441 gttcacccgt gatgtccggg agcggacatt gtaccgaaaa aaacggcgaa aaaactcgat
     1501 tagactttgc gtccgtgatt tattttcagt gagctggctc aatttctaat ttcccataga
     1561 aactcgatta gaggtagttg agagttgaca gttcagagtt aacagtaaag gcgaaaagcc
     1621 aaggcgccac cgtttgtttt cggtgagggc ggccgttcgt acatctgggc aaacgatctg
     1681 ttcagaaagg cgcatttcag tcccgattag aaattcttat gcgctgcgtt ttcaggcagt
     1741 tgactctttg cgacttgtgt tttgtgtacc gattagaatt ctcttcgtca tggagtgtgt
     1801 aagccattgt cttcgtttgg gttagcggcg aattgtgtgc aaaagttgcc atggcgtttt
     1861 tgacctgtgt ttatcagggc gtgccagttt tctgtcggtg aggacagcct ggccagcaac
     1921 aaggccggcg cattccttct ttaagttccg tgcgtacgcg ctcaacgtgc tgccttctgg
     1981 tttccggttt cttgacggag ataacccagc agatccactc attgcgcgcg agtggggtga
     2041 tgtcctccca ttttgcccgc gcggccgggt cggaaatgag aacttttcgt aaatccgctg
     2101 gtacgctgtg taccgcgccg gcggagatcg ctttcttggt cattttcctg gttaccgatc
     2161 tcattctaca tgtcacgcga tttgcaaatc aacgaaaccg tcctgccacc ttcgctggaa
     2221 ctccagctac ggcgcgccga acagtggctt ggcgttggtg cgattgcgtt tccgccgatt
     2281 cccggagaaa acggtgcata aacgaaaaaa gccgcccgag gcggccttgc acttctcttg
     2341 cgcgaattat ctggctggct gtgcagccga atagagatgt gtaccataga gttaacaata
     2401 agtcaagaag aatttaagct gtgaggccgc atttagtccg gtataaggtc aaggagctgc
     2461 ttacgaagag gcctttgcca gactccggtc gagatatcgt tcatgatgtc aattatggac
     2521 gctaaacccc ttgtcacttc cttactgtac tgtgacagtt caatgtcgtc atccaatgct
     2581 cgcggatgca cgagagcgtc tcggaaccgt ttgaattcca tgaatgtgga ccatgtgggg
     2641 cttctaaagt cgaagctcgg tgtaaatcgc cgcaggacgc accttatttt gtcgtccggc
     2701 ctatggtatt cggttttctc tacgaatcct ttggcagcgt caaactttag cgtcttgtcg
     2761 ttaagaaacc ggatttcatc cttcggtatg ctatttcctt tcgcgaaact gtctccgata
     2821 tagttgataa atgattcgat agctcccgag gcgtaaaata cggatgctcg gtaaaggcgt
     2881 cgagcctggc ggtcgttgtg ttgtttggaa agcttttgtg ccgcaagaag aaaatgatgt
     2941 gaatcttgga gatagtcttc aaacatgacc tatagctttc ctagaaccgt tttggcatcg
     3001 tggctcgctt tcttagcctc gtaagattgt atggcggcgt tagctagttc ttttgtgatc
     3061 cttggcttgc ccttgttagc aagaacccag aagacctcga gcattcggcc gagctcctta
     3121 gggctgctac gtgtgcctag ccctcgataa gtgtcagggt cggttccctt cagccttgcc
     3181 tgcgagagcg cttttgcatt taatgggtcg tctggaggag agacgttgtc ggggatggag
     3241 taaagattct ttagcagagc tggatcgcgc tggaataaac cggcagcata gccccaactc
     3301 gcgacgacgg acaacgatag cgctttacgg gcgaactctg cgttatcgtc ctcgtcccga
     3361 tctgaaacgg tctttctctg tagtctatgg aggcgggcaa actgtttgcc catttgttcc
     3421 agagcaggtt ccgaccaagc gatcccgctt ctgattttca tgtactcctc gtctatgccc
     3481 ccggacttgc agagtattgg ctggtggaaa tctcccacgt cctttacgtc ttttcccaag
     3541 cagaagttca agatgagtgt ccttgcggcc ctgaccgtcg gtacgtcggg gcttcgtcga
     3601 tccgagaagt cggtcgactt ctccaataaa cctacccttt ggcaccaatc ccgcaggtca
     3661 gtccccaata actgttcctt catccggtcc agcagatcat ttggtacggc gattgcggtg
     3721 ttgttaattg tcgcgatctc gactttttgg tcgcgagaga gctcgaaata tatccgcacg
     3781 ccatgaagag ttgcgccctt cgctggcaat gcttttgtta ttgccctcag cctatgctgg
     3841 cctccatata cttttagggg tttctttgct cggtacttcg tgtcgtattc caggaccatg
     3901 tcctcgaagc ttctaccctt gattgcatcc tgctccatga gcttgtaggc tgcttcgtct
     3961 tccgttattt ctcgattgag cttatagatc tcgtcctctt cgtccccgtc caggctcgct
     4021 tcggagtcgc aggctccggc gagaaccttg ccgtcgagat ggcagctcag gtagtaagcg
     4081 ccgcttttct cgtcgagaaa aaggagggcc ttgccgccac tgctgacttc gtgcggcgga
     4141 aacttctcta ggaattccgc gaccttctta gttactggtg cgggcggatg cggtcttgag
     4201 ggcaggacaa gcggctcaga catttatgct ctcccgaaaa gtgaatcata tcaccacggt
     4261 agattaccgc acagtacatg ttcgtcgcgg caccatcgcg tcagttttgg gaggcgggtc
     4321 gagatagacg aggtcgacgg attcgtcctt gatgtagcgg cggagaacgt cgaggttatc
     4381 gccgtagtaa agctgattca tgtcagcgga ttatatagaa aaacgggagg tatgcgagga
     4441 gtgattggta acgagttgga caacgaatac attgcgcgcg cacgaagtgc gcaggaacgt
     4501 ccgcgcaaaa ggaaggaatc gttttgtgac ggcctttcgg cgatgccgga aaggcgtcga
     4561 ggcgagatcc ttcgtcgcga aatgcgctcc tcaggatgac aatgcgaggc aggggccgca
     4621 gaacttcgga gggcgaagct ctgcgctaca aaaacgggaa gcgcgtgcta acatcgagcg
     4681 tttgtaattt gcgcggcgcg gcggatttga tcgcgctgcg cgcgacgggc gcggcccttc
     4741 gtgcctcagg gtgaacgggc agggtaaacc ctgcccctac gaaaaagcaa aagcagatcc
     4801 ctcggaccgt ctccgaaata cggagccggc ctcgggatga caacgagaag cgaaatgggc
     4861 gcagcccttc gtgcctcagg gtgaacgggc agggtaaacc ctgcccctac gaaaaagcaa
     4921 gagcagatcc ctcggaccgt ctccgaaata cggagccagc ctcgggatga caacgagaag
     4981 cgaaatgggc gcagcccttc gtgactcagg gtaaacgggc agggtaaacc ctgcccctac
     5041 gaaggcagca gcaggtccct cggaccgtct cctaagagac ggagccggcc tcccttcgcg
     5101 aaggcactca gggcaggcgg gatgacaaca ggaactagga gcggacgagc gaaagtatgc
     5161 aaacggggat tattgggctg ccgcaggttg ggaagacgag tttgtttcgc attttgactg
     5221 gcgtgcgcgt ggatgccaag acgcagcccg ggcagatgca cgtgggaatc gcgcgcgtgc
     5281 ctgacgcgcg aatcgtgaag ctcgcggaat tgttcaagcc caagaaaata acttatgcga
     5341 ccgtcgaata cgtggacatt ggcggcttgc agaaggatcg cgcgaaggat tcggcttcgc
     5401 tcgtcccgtt gcgcgaagcg gacgcgctga tgcacgtcgt gcgcctcttc gaggatccgt
     5461 cgatgccgca cccggcgggt tcgctcgacg cgctgcgcga tatcgagagc gtcgaaatcg
     5521 agttgatgct gaatgatttg gagcaggcaa cgaagcgtct cgagcgcgtc gaaaaagatt
     5581 tgaaaaagaa gaaagacccg cttgtggaag ccgaaaaaaa cgtgctcgac cgttgcatca
     5641 aagcgctcga atcggaaacg ccgctgcgcg aactcgactt caagcccgac gagctgaaaa
     5701 tcgtgaacgg tttcatgttc gtgtcgcgca aaccgatgct cttcgcgctg aatctgggag
     5761 acgacgaagc agcggacatg cagggcgcgg tggcgcggca taatttgtcg aagctcgcgg
     5821 gaaagacgcg aacagc
//
LOCUS       DATNDT010000230         7889 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3453085, whole genome shotgun sequence.
ACCESSION   DATNDT010000230 DATNDT010000000
VERSION     DATNDT010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7889
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3453085"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2007
                     /locus_tag="VN788_07440"
     CDS             <1..2007
                     /locus_tag="VN788_07440"
                     /inference="COORDINATES: protein motif:HMM:NF012956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HXU20398.1"
                     /translation="AGWGTVTYRQNTELQIEAWHWNPRGTWSDPAKKEGYFVGDAEPT
                     GKQIVDSWAYPLPHRGATLGDGNGWSRITDGDPSTYWKSNPYLTKNFTGEDDSLHPQW
                     VMIDLGARVPIDAIRIAWANPYATRYYVQFWTGDLEPFYAGINRGTWQTFPMGSVSRG
                     HGGTSTLRLADWDTSVRYLRIWMKESSNTCDTHGPQDKRNCVGYAINELYIGTLTPDG
                     QFTDIVKHSPNRQQTITWPSSVDPWHAASDLDYGRGDQVGFDFFFHSGVTRGLPTMVP
                     IAMLYSTPEDAANEIAYLYKRKYPISWIEMGEEADGQRVLPEDYAALYIQFARAIHKL
                     VPQARLGGPPFEGTSGDVDSWADANGRVSFLGRFLDYLKAHDDLQDFTFFSFEHYPCM
                     GTGKCRDWTSLYYEPEWVDHVVQAWKDNGLPPNIPFFMTEGNDLGEGSPETVKSGLWL
                     ADYVGAMMTAGAGGTYYFHYIAQPGRGGRGFLSVDEENHPTYSPQYLATQVITQDWVQ
                     PVDKIHKLYRATSDVKDDSGNVLVTAYPVERPDGNWSVMLINKDENNDHDIKVSFTDG
                     ATKESRYFTGTVDRVVFGPNEYQWHPDQTPVTTSLTSASISSDAPQEDGEGAGRGRGR
                     GGFDGHADPDGPASKSTLTAAGQDTVFVLPKASIIVLRGKIAGE"
     gene            complement(2527..3765)
                     /locus_tag="VN788_07445"
     CDS             complement(2527..3765)
                     /locus_tag="VN788_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020261800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU20399.1"
                     /translation="MSEKRLGIGIVGSGFITRFHLQSMVAVRDVEVRGLWSPNREHAA
                     SAANLARSLDLGDAVAYGSIAEMVTAAGIDAVWLMGPNHTRVENLEEIVAAAAKRSVP
                     LKGIACEKPLARNVREAQQMLQIVTKAKIPHAYMEDQIFTPAIDTGRKILWARGASAG
                     RPYLARAAEEHSGPHAPWFWMGDLQGGGVLNDMMCHCVLVVQHLLTKPGQPYTTVKPL
                     RITGHIASLKWSRPEYARDLAKRMGKGVDYLKHPSEDYASVTIEYETDTGTMAIGEAT
                     TSWSYVGAGLRLSCEMLGPEYSMNWNSLNTGLNVFLSRELRGKEGEDLVEKQNAESGQ
                     MPVLADEANAYGYSAENRHLVRSFLHGTKPALTFEDGVEVVKILMAAYQSAEEGKTIA
                     LPARGLESYKPLVARGKWKP"
     gene            complement(3968..>7889)
                     /locus_tag="VN788_07450"
     CDS             complement(3968..>7889)
                     /locus_tag="VN788_07450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU20400.1"
                     /translation="RAMTSFQKCIRVLCFALSLAFLAITGVRAQETADIVGTVTDASG
                     AVLPGATITLTNTGTSVSRTATTSSSGDYVFNLLQVGTYAVKVEMKGFKTFTAPSITI
                     AAGDRARVDAKMEVGDITQTVEVSGTVAPALQTDTSTIGSLVPSRSVEDLPLNQRNII
                     KLVQLSAGATEGQPNSIAAGNRPDDRRMTDAVSINGQDDSTNQNMIDGFDNNTRIIGS
                     IGVRPSIDAIQEVNITTNKYDASVGRTGGGVVDVITKSGTNSFHGSAFEFLRNKALNG
                     NPNWNFNEALNLEAIKAGMTGVTDPHSVAAPNPAFRQNQFGGSVGGPIIKNKTFFFVD
                     YEGYSYAQGLAASFYTVPTLCEKGLAVCPDGKTQLGDFSDNPTVSLPASQGGSTQLCS
                     TKPPASQPQLLYGSATCPYVTVPSASITPIGLSFFNMYPLPNTGAPGALTNNYTSAPV
                     KFQHAKTIDGRIDEHFSDKDTLFGHVTYNGLDTLNPNGFPDVYLNPSTGALASAGGSG
                     ALKVTPVVTSYAGPNNEDQYFVAASYVHVYNPNLVLNLKAGVMRSQILSYPANQGTEI
                     SNKLGFPCSATSCVNYSSSASLVGSSGLTRPTVRGINGGTSYSIIGDSNFVPLGYWDT
                     SLQYYAILTQNKGSHSIRYGLGLIRRRAGVAQSNAAQGGFTFNGSYTGEPMGDLLEGL
                     ATASQRNNSLVQDGFRTWEPSVYIQDDWRARPWLTLNLGVRYDIFTPFTEVHGRISNY
                     DPYIGLLVSPSLPGTQQSSNTAMVPTPYADIAPRFGFAATLPHNMVLRGGFGMTFMPQ
                     NYGSNYYFPNAPFTFSSSCAIQNRGGTANSCATAQYDGPVGEFSSGIASNYGTPYQSQ
                     YCNGPCSTAKSSTLGEVGGTSFALGLPVPYLNVALATNTANYAGTTLQAVQTNQKESY
                     LEQFNLQLQKQFGANVVSVAYVGELGRRMGSFLPGPVNQNQAANPTETLGSNGQYLPM
                     SVGGATNDGFGTLPGFPYLASVNVGESSNLGTSAYEGLQATFVRRFSKGLTANINYTW
                     SHTMDNFLGSQACVESMFAAPEPCWVDEANGAGPSLVPPSGATPATFPVTSCANEGPK
                     VCKNYWGWQQYAWGNATNDVQDRIAWGINYDLPFGNSFTGAKGILLKGWATNLSGSWQ
                     TGLPFTPSPASNNDAIGGAGYLDQICSGKAAHPSILDWYNYNCFVNPTPGTLGNGRFG
                     ALFGPHQKRLDFSLFKTFALTERVNLQFRTEVFNLFNQTNFGQPSASEAFTGTIAGAS
                     GPYPNAAPPVNISPPGGSHTTGEITSVSGNWNPRQIQFALKVLF"
BASE COUNT         1753 a         2036 c         2368 g         1732 t
ORIGIN      
        1 gcgggttggg gcacggtgac gtatcgtcaa aatacagagc tgcaaatcga ggcatggcac
       61 tggaatcctc gcggtacctg gagtgatcct gcaaagaagg aaggctattt tgtgggcgat
      121 gccgagccca caggcaaaca aattgtagat tcgtgggcgt acccgcttcc gcatcgcggt
      181 gccacgcttg gggacggaaa tgggtggtcg cggattacgg atggagaccc gagcacatat
      241 tggaagagca atccctacct gacgaagaat tttacgggcg aagacgattc actccatccg
      301 caatgggtga tgatcgactt gggtgcaagg gtgccgattg atgcgatccg catcgcctgg
      361 gctaatccat acgccacccg ctattacgtg caattttgga cgggcgatct cgagcctttt
      421 tacgcgggaa tcaacagggg tacatggcag acgttcccga tgggcagcgt atcgcgaggc
      481 cacggcggga cttcgacgct gaggcttgcc gactgggata cttccgtgcg gtaccttcgc
      541 atttggatga aggagtcatc caacacatgt gacacgcacg ggccgcagga caagcggaac
      601 tgcgtcggat acgcgatcaa tgagctctat atcgggactt tgacgccaga cggacagttc
      661 acggacatcg ttaagcattc tccgaatcgg cagcagacga ttacctggcc gtcatcggtt
      721 gacccatggc acgccgcgtc agatctcgat tacggtagag gcgaccaagt cggatttgat
      781 ttcttcttcc attcaggcgt tacgcgcgga cttcccacga tggtgcctat cgcaatgctc
      841 tattcgacgc cggaagacgc cgcgaatgag attgcgtacc tctacaagcg gaaatatccg
      901 atctcctgga ttgagatggg tgaagaggcg gacgggcagc gcgtccttcc ggaggattac
      961 gctgctttgt acattcagtt tgcccgggcg attcacaagt tggttcctca agcgcggttg
     1021 ggcgggcctc cgtttgaagg aacgtctgga gatgtggatt cctgggcaga cgcgaacggg
     1081 cgcgtctcgt ttttgggacg gttcctcgat tatctgaagg ctcacgacga tctgcaggac
     1141 ttcacgttct tttcgtttga gcattatcca tgcatgggca cggggaaatg ccgcgattgg
     1201 acatcgctct actacgaacc ggaatgggtc gatcacgtgg tgcaggcgtg gaaggataat
     1261 ggcctgccgc cgaatattcc ttttttcatg accgaaggga acgatctggg agaagggagt
     1321 ccggaaacgg ttaagagtgg gctttggttg gctgactatg tcggcgcgat gatgaccgcc
     1381 ggcgcgggcg gcacctacta tttccactat attgcgcagc ctgggcgagg cggacgaggt
     1441 ttcctcagcg tggatgagga aaatcatccg acgtattcgc cgcaatatct cgccactcag
     1501 gtgatcacgc aggattgggt ccagcccgtc gataaaatcc acaaactgta cagagctacg
     1561 agcgatgtga aggacgactc aggcaacgtt ctcgttacgg catatccggt ggagcggccc
     1621 gatggaaatt ggtccgtgat gctgatcaac aaggacgaga acaacgatca cgacataaaa
     1681 gtctcgttca cggatggcgc tacgaaagaa agtcgatatt tcacgggcac cgtcgaccgc
     1741 gttgtgttcg ggccaaatga ataccagtgg catccagacc aaacacccgt cactacatcg
     1801 ctcacgtctg cttctatttc ttccgacgca ccgcaagagg atggtgaagg tgctggccgc
     1861 ggacgaggtc gaggtggctt tgacggtcac gccgatcctg acgggcccgc ctcgaagagc
     1921 acgttgacgg cggcaggaca ggacacggtc tttgttctgc cgaaggcatc gattattgtg
     1981 ctgcgcggaa aaatcgcagg cgagtgatca ttatggtgaa atctgcgcta tcaatccaag
     2041 gattctcgaa aagcaactgt ttgagcctct ccttaaacgt aaaatggagc gctgcaccca
     2101 aaggcggctc gaagactttg cgccatcact cctcaataat ttgaaaatat tttcggttta
     2161 aaagtgcttg ctttctggta accaatatgt tattatcggc gcgtgacctt ggctgaatca
     2221 tttcggacgc tgattgaaag cccccaacct cctaatgtgc cgttctaatc gcaactgcac
     2281 agaattaaaa atcggcgtaa gtccatgaaa acaaagggag ggggccagtt ctaatcgcaa
     2341 tagtcagcag ggggaagcga aaggcgaaga ggccccaagc catcacgcga tttgggtacg
     2401 cgggcagccc taagcgctga aaacgcaaac cgtccgaagt tttcaggcaa aaggtgccga
     2461 cctggcgagc tgaactcgga tacgggcccg aatagcatcc cgagggcggc gttgttctct
     2521 agcttttcac ggtttccatt tccctcgagc aacgagcggc ttatacgatt ccagcccgcg
     2581 cgcgggaagc gcaatagttt ttccttcctc ggcgctctga tatgccgcca tcagaatttt
     2641 tacgacctcg acgccgtctt caaaggtgag cgcgggcttc gtgccgtgga gaaatgagcg
     2701 gacgaggtgt cgattctcgg cggagtagcc ataggcattt gcttcgtcgg caaggaccgg
     2761 catttggcca ctttcagcat tctgcttttc gacgaggtct tctccctcct taccgcgcaa
     2821 ctcgcggctg agaaaaacgt tcaaccctgt attcagagaa ttccagttca tggaatattc
     2881 cgggccgagc atctcgcagg aaaggcgcaa gcccgctccg acgtagctcc acgacgtggt
     2941 cgcttctccg atagccattg tccctgtgtc cgtctcgtat tcgatcgtga ccgacgcgta
     3001 atcttccgaa ggatgcttga gatagtcgac gcctttgccc attctcttcg ccaggtctcg
     3061 agcatattcg gggcgagacc atttcaggct ggcgatatga ccggtaatgc gtaacggttt
     3121 gacggtggta tacggctggc cgggcttggt cagaagatgc tgcacgacga ggacgcagtg
     3181 gcacatcatg tcattgagta caccgccgcc ctgaaggtca cccatccaga accatggcgc
     3241 gtgtgggccg ctgtgttcct ccgcggcgcg agccagatac ggccggccgg cagaggctcc
     3301 gcgagcccac agaattttgc ggcccgtgtc gatcgccggc gtgaagatct gatcttccat
     3361 gtaagcgtga ggaattttcg ccttcgtcac gatttgaagc atctgctgcg cttcgcgaac
     3421 gttgcgcgcg agcggcttct cacaggcgat gcccttcagc ggaacagatc gtttcgccgc
     3481 tgcggcgaca atttcctcca ggttctcaac tcgcgtgtga ttcggcccca tcagccagac
     3541 ggcgtcaatg ccagcagccg taaccatctc cgcgatggaa ccataagcca cggcatcacc
     3601 caaatccagc gaacgagcaa ggttcgccgc gctggcggcg tgctcgcgat tggggctcca
     3661 tagaccgcga acttccacgt ctcgaacggc taccatcgac tgcaaatgaa accgagttat
     3721 aaagccgctg ccgactattc cgattccaag tcttttctcg ctcatgaggc ctcgtttgaa
     3781 gtttgcgaac gaatgacgtt acgacacggt gcggaccggg tcaacggcgg gtttacgcgc
     3841 gaatagaaaa tgtgcggatt cttgttggga acgggcagta attgagagtc ttgcggacgc
     3901 caacattatc gggactgaaa agtggccact gacaatcggc gatcgcctgc cagtggccct
     3961 ttccgtttta gaacaggact ttcagcgcaa actggatttg acgtggattc caatttccgc
     4021 tcacagatgt gatttcaccc gtggtgtgtg aaccgccggg cggcgagatg ttgaccggag
     4081 gagcagcatt tggataaggc ccactggcgc cagcgatagt tcccgtgaag gcttcgctcg
     4141 cgctaggctg gccgaagttt gtctgattga agagattgaa cacctccgtg cggaactgga
     4201 ggttcacgcg ttcggttagc gcgaaagtct taaagagcga gaagtccaat cgcttctggt
     4261 gtggcccaaa aagcgcgccg aagcgaccat taccaagagt tcccggcgtc ggattgacga
     4321 agcaattgta gttgtaccaa tccaggatag aggggtgagc cgctttgccg gagcaaatct
     4381 ggtcgaggta gcccgcgccg ccgatggcat cgttattcga agccggtgaa ggcgtgaagg
     4441 gcaaaccggt ctgccaagaa ccggaaaggt tagtagccca ccctttcaga agaattccct
     4501 tagcgccggt gaaagagttg ccaaacggca gatcatagtt aatcccccaa gctatgcggt
     4561 cctggacatc gttggtcgca ttaccccaag cgtattgctg ccagccccaa tagttcttgc
     4621 aaaccttcgg gccttcgttg gcgcaagacg tgactgggaa ggtggctgga gttgctccac
     4681 tgggcgggac gagagacggt ccagctccgt ttgcttcgtc aacccagcag ggctctggag
     4741 ccgcaaacat ggattcaacg catgcctggc tgccaaggaa gttgtccatt gtatgcgacc
     4801 acgtgtaatt gatgttcgct gtgaggccct tcgagaagcg gcgaacgaaa gtcgcctgaa
     4861 gaccttcgta agctgaagtg ccgaggttcg acgactcgcc aacgttaacg ctggcgagat
     4921 atgggaagcc gggtagagtg ccaaaaccgt cgttggtcgc tccgcccaca gacatcggca
     4981 agtattgtcc gtttgagccg agtgtttctg tcgggtttgc cgcttggttt tgatttacag
     5041 ggccgggaag gaagcttccc atgcgccggc cgagttcgcc cacgtaggcg accgagacaa
     5101 cattcgcgcc aaattgcttt tggagttgaa ggttgaattg ctcaagataa ctttctttct
     5161 gattcgtctg aacggcctgg agcgtggtac cggcgtagtt ggctgtattt gtagcgaggg
     5221 ccacgttcag ataaggaacc ggcaggccta aggcgaagga tgtaccgccc acctcaccca
     5281 aggtgctgct ctttgcggtg ctgcagggac cattgcagta ttgggattgg tagggcgtac
     5341 cgtaatttga agctatcccg ctggaaaact caccgacagg accgtcatat tgagcggttg
     5401 cgcaggagtt ggccgtaccg ccccggttct gaatcgcaca gctcgacgag aacgtaaatg
     5461 gagcgttggg gaagtagtag ttcgatccgt agttctgcgg catgaacgtc ataccgaagc
     5521 cgccacggag aaccatattg tgaggcaaag tggccgcaaa tccgaatcgg ggcgcgatat
     5581 ctgcgtaagg tgttggaacc atcgcggtat tgctggactg ctgagtcccg ggaagtgacg
     5641 ggctgacgag caaaccgata tagggatcgt agttcgagat gcgaccatgg acttcggtaa
     5701 agggagtgaa tatgtcgtag cgaacgccga ggtttagagt gagccaaggg cgcgcgcgcc
     5761 aatcatcctg aatatagaca ctcggctccc aggtacggaa gccgtcttgc accagcgagt
     5821 tattgcgttg cgacgcggtt gcgagtcctt ctagcaggtc gcccatcggc tcgcccgtat
     5881 aagaaccatt gaatgtgaaa ccaccttgag ccgcattgct ttgggcgacg ccggcgcgcc
     5941 gacggatcag acccaagccg tagcgaatgc tgtgggatcc cttgttctgg gtgagaatcg
     6001 cataatactg aaggctggta tcccaatacc ctagcggaac aaagttcgaa tcgccgataa
     6061 tggagtagct ggtaccaccg ttaatgcccc taacggttgg ccttgttaga cccgaggagc
     6121 caacgagtga agccgacgaa gagtaattca cgcaggacgt ggcgctgcag gggaagccca
     6181 gcttgttcga gatttccgtt ccctgattgg ctggatatga aaggatctga ctgcgcatca
     6241 cgccagcctt caggttgagg acgaggttcg ggttataaac gtggacgtag gaagcggcaa
     6301 cgaagtactg atcttcattg ttgggaccgg cataggaggt aacaacgggt gtgactttca
     6361 aggctcccga cccaccggca gaagccagtg cgccggttga gggattgagg tagacgtccg
     6421 ggaaaccgtt tggattaagg gtgtccagcc cgttataggt gacgtgaccg aacagagtgt
     6481 ccttgtcgct gaagtgctcg tcgatacgtc cgtcgatcgt cttggcgtgc tggaacttta
     6541 cgggtgcgga cgtgtaattg ttcgtcaaag ctccgggtgc gccggtgttc ggcaacggat
     6601 acatgttgaa aaacgacagg ccgattggag taatgctggc ggagggaacg gtgacatacg
     6661 ggcacgttgc tgagccgtac aaaagctgag gctgactagc cggtggcttg gtggagcaga
     6721 gctgggttga accgccttgc gaagccggca gcgagacggt ggggttatcc gagaagtcgc
     6781 cgagttgggt cttaccgtcg gggcagacgg ccaagccttt ttcgcagagc gttggcaccg
     6841 tatagaaaga ggcggccaag ccttgcgcgt aagaataacc ttcgtaatcg acgaaaaaga
     6901 aggtcttgtt tttgataatc ggaccgccga cgctgccgcc gaattggttc tggcggaatg
     6961 ccgggtttgg cgcggcgact gaatgaggat cagttacacc ggtcatgcca gccttaatgg
     7021 cctcaaggtt gagcgcttcg ttgaagttcc agtttgggtt cccgttgaga gccttgttgc
     7081 ggaggaactc gaaggctgag ccatggaagc tgtttgtccc cgacttcgta ataacgtcga
     7141 ccacgccgcc accggtccga ccgacggaag cgtcgtattt attcgtcgtt atgtttactt
     7201 cctgaatcgc gtcaatggag ggacgaacgc cgatcgaacc aatgatccgc gtgttgttat
     7261 cgaacccgtc gatcatgttc tgattggttg aatcgtcctg gccatttata gatacggcgt
     7321 cggtcatacg ccggtcatca gggcgattgc cggcggcgat cgagttgggt tggccctctg
     7381 ttgcgccggc ggaaagttga actaacttga tgatattacg ctggtttaaa ggcaggtctt
     7441 caacgctccg tgagggcaca agggagccga tggtagacgt gtccgtctgc aaagccggag
     7501 caacggtacc ggatacttcg acggtctgcg taatatcgcc gacttccatc ttggcgtcca
     7561 cgcgagcgcg gtctcctgcc gcaatcgtga tgctgggcgc ggtgaaggtc ttaaatcctt
     7621 tcatttcaac ttttacggcg taagttccga cctgcaagag gttaaagaca tagtcgccgc
     7681 tgctgctggt tgtagcggtg cgcgagacgc tcgtaccagt gttggtgagg gtaatcgttg
     7741 caccggggag aacggcaccg ctggcgtccg ttactgtgcc gacgatgtcg gcagtctcct
     7801 gcgcgcggac tccggtgatg gcaaggaaag caaggctgag cgcgaagcaa agtacacgta
     7861 tacacttctg aaaagaagtc atggcacgc
//
LOCUS       DATNDT010000231         5449 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_898388, whole genome shotgun sequence.
ACCESSION   DATNDT010000231 DATNDT010000000
VERSION     DATNDT010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_898388"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="VN788_07455"
     CDS             complement(<1..1568)
                     /locus_tag="VN788_07455"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU20401.1"
                     /translation="MKTFLYALVLSILLLPASARLFAQDVTYQQLLHADETPQNWLMY
                     GGDYSSQRFSRLTQINRENVHNLKPVWIYQPDRPLRPVETSPVVVNGIMYVTEPLSTV
                     IALDARLGTEIWSWSAKLPEKIYTIGVHRSNRGVAILGNTLYMETLDAHLVALDAITG
                     AMKWNVHVDDNNEGYAMTGAPRVLDGKVIIGVSGGDVGIRGFLDAYDAKTGKRLWRLW
                     TIPAPGEPGSETWGKDMAGTGGGATWGTGSYDPELNTLYWTTGNPAPDYNGHNREGDN
                     LYTNSLLAIDPDTGKMKWYFQYTPHDTHDWDSIQVPVLFDETIDGKPRKLLAQANRNA
                     FYYVIDRVTGQFITGVPFVKETWAKGLDAKGRPIELPDIEPTPKGRLVYPDGHGGTNW
                     NSPSYSPQTKLFYVSARETGAYTVAGEPKVDFPSFGGGGRINLPADQVYGAIRALEAT
                     SGKMKWEYRMLAPAEASTLATAGGLVFAGSNEGNFFALDAATGKLLWEFTAGGSDSES
                     NLITYEAGGKQYVIG"
     gene            complement(1565..2389)
                     /locus_tag="VN788_07460"
     CDS             complement(1565..2389)
                     /locus_tag="VN788_07460"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU20402.1"
                     /translation="MVLLAWTLMFNSSRVAQAAGQESPPAQAAPASRAAKAPPDLEDG
                     KQQFAQNCSTCHGVDAGGQEGPDLHGVPESLGDSAVQGIIRRGIPGTAMPSFSTVTDK
                     QAADIVAYLRTLSGTGTAEKAIGDPQKGEALYNSSGCSTCHMIDGQGGSIGPDLSRIG
                     SSRGPTNLKGRLQDPGANLPQVGGGIFGGSLTQYLMFRAVEKNGQTIEGIRVGEDSFT
                     IVLRDAGGKFHGLWKPDLRSLEKEPGKSFMPSFKDTLSDAQMDDLVAYLMTLKRAQ"
     gene            2722..4362
                     /locus_tag="VN788_07465"
     CDS             2722..4362
                     /locus_tag="VN788_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXU20403.1"
                     /translation="MAENSENIGSALTGDAAEAALPRFSPATWRPSFNPWLIAVIVAL
                     AAFMEVLDTSIANVALPYMAGNLGVSNDESTWVLTSYLVSNAIVLPISGWLVTVVGRK
                     RLFMTCLFIFTVSSLLCGIAPSLGALILFRILQGAGGGGLQPLAQSILADSFPPEKRG
                     IAFAVYGITVIVAPTIGPTLGGWITDNYTWRWIFFINLPVGILALLLVYQFIEDPPWA
                     HRIKDALARIDYIGISLLTLGVGALQVMLDKGQEDDWFGSRFILTFFILAAIGLVSLF
                     IWEWFAKNSVVDVRLFKNLNFLGANVMMFTLGVIYFSSLVLMPQYLQTLMGYTAETSG
                     FVLTAGSAVLLFTMPLIGVLSGKFQARYLIAFGWLSLVFGFYYSAVRLDLGISFGVAS
                     VIRVVQVIGLPFLFVPINLASYVGMPPDKSSSVSGLINFMRNIGSSVGTSLVTTLIAR
                     RAQFHQVHLVGNLASGNATLAGTAAALAAHLTASGVNAERAMEQAYALIYRTVIAQAS
                     TLAYIDTFFVLTAGAAIMFLLSFALKKNDPSGRRVVME"
     gene            4445..5107
                     /locus_tag="VN788_07470"
     CDS             4445..5107
                     /locus_tag="VN788_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HXU20404.1"
                     /translation="MIRIITIEREYGSGGAEIAKRVAARLDWKLWDQLLTNEIARLMD
                     CDCRVVEEHEEKRDPLYYRLFKSFMRGSFEGSLNEPRLRMADTDCIREVTERVVKDVA
                     ERGQSVIVGRGSAYYLQGRSDAFHVFVYASLGEKVQRLRATGKSEREAVQLAETVDLD
                     RAAFIKQYFGVEWPDRHRFHLMVNSTIGTDAAVDLILEGTGMQDKPQTVSAPPALKVE
                     KA"
BASE COUNT         1106 a         1673 c         1463 g         1207 t
ORIGIN      
        1 ccgattacgt actgcttgcc gcccgcttcg taggtgatga gattcgattc agagtccgat
       61 ccaccggcgg taaattccca caacagcttg ccggtggcgg cgtccagcgc gaagaaattg
      121 ccttcattgc ttcccgcgaa aaccaagcca cccgctgtcg caagcgtgct ggcctcggcg
      181 ggagcaagca tgcggtactc ccacttcatt ttgccgctgg ttgcttccag cgcgcgaatc
      241 gcaccgtaaa cctggtctgc cggaagattg attcttccgc ctccaccgaa gctgggaaaa
      301 tctaccttcg gctcgcccgc aaccgtgtat gcgcctgttt cgcgcgctga gacgtaaaac
      361 aatttcgtct gcgggctgta cgacggacta ttccaattcg tgcccccatg gccatcggga
      421 tagaccaatc gtcctttggg agttggctcg atatcaggca actcgatggg ccggcctttc
      481 gcgtcgagcc ctttcgccca cgtctctttt acaaacggga cgccagtgat gaactggcca
      541 gttacccgat cgattacgta ataaaacgcg ttgcggttgg cctgcgccag aagctttcta
      601 ggctttccgt cgatcgtctc gtcgaacagc acggggacct gaatcgaatc ccaatcatgc
      661 gtgtcgtgcg gcgtgtactg gaaataccat ttcatttttc cagtgtcggg atcgatggcc
      721 agcagcgaat tcgtgtagag gttgtcgcct tccctgttgt ggccattgta atcgggcgcg
      781 ggatttccgg tggtccaata cagcgtgttt agctccggat cataagatcc ggtgccccag
      841 gtggctcctc cgccggtgcc ggccatatct tttccccaag tttcagaacc aggctcgccc
      901 ggcgcgggaa tggtccacag gcgccaaagc cgcttccccg tcttcgcgtc ataggcgtcc
      961 aggaaaccgc gaatgccaac gtcgcctcca gaaacgccga taatcacctt tccatcgagc
     1021 acgcggggcg cgcccgtcat cgcgtaccct tcattgttgt cgtccacgtg cacattccat
     1081 ttcatggcgc ccgtgatggc gtccagtgcc accagatgcg catccagcgt ctccatgtaa
     1141 agcgtgttgc cgagaatcgc cactccgcga ttcgagcgat gcactccgat ggtataaatt
     1201 ttctccggca gtttggcgct ccagctccag atttccgtgc cgagccgcgc atccagtgcg
     1261 atcactgtgc tcagcggttc ggtgacgtac atgatcccgt tcaccaccac cggagatgtt
     1321 tccaccggcc gaagaggacg gtcgggctga taaatccaca ccggcttcag gttatgaacg
     1381 ttttcccgat tgatctgcgt aagccttgag aagcgctgcg aagaatagtc gccgccatac
     1441 attagccagt tttgcggcgt ttcgtcggcg tgcagcaatt gttggtaggt cacatcctgc
     1501 gcgaacaacc gcgccgaagc cggcaaaagt aaaatcgaca gcaccagcgc atagagaaag
     1561 gttttcattg tgctctcttc agagtcatga gataggccac cagatcgtcc atctgcgcgt
     1621 cagacagagt gtccttgaag ctcggcatga aacttttccc cggctctttt tccagggagc
     1681 gcaaatcggg cttccagagt ccgtgaaatt ttccgccggc gtccctcagg acaatcgtaa
     1741 acgagtcttc accgacgcgt atgccctcaa tagtctgccc gttcttttca acggcgcgaa
     1801 acatcagata ttgcgtcaag ctcccgccga aaatgccgcc gccgacctgc ggcaaattcg
     1861 cgccgggatc ctgcaatctc cctttcaagt tcgtggggcc gcgactggag ccgattcggc
     1921 tcagatccgg tccaatgctg ccgccctggc cgtcgatcat atggcacgtg gagcacccgc
     1981 tcgaattgta gagagcctcg cccttctgcg ggtcgccaat tgctttctcc gccgtgcccg
     2041 tgccgctcag cgttcgcaga taagccacga tgtccgccgc ttgtttatcg gtcacggtgg
     2101 aaaagctcgg catcgcggtg cctggaattc ctcggcggat gatgccttgc acagccgaat
     2161 cgcctaagct ctccggaaca ccatgcagat cgggaccctc ttgccctcca gcgtcgactc
     2221 cgtggcacgt cgagcagttc tgcgcaaatt gctgtttgcc gtcttcgaga tcgggcggcg
     2281 cctttgcagc ccgagaagcg ggcgccgcct gcgcaggcgg agactcctga cccgcggcct
     2341 gagcaacgcg agaagaattg aacatcagag tccatgccag gagaaccatg gccgcaagcc
     2401 gcaagcaccc gattgcattc acaccgcacc cccgctcaca attcgcctct cagcatgtct
     2461 cgtttgttgc aaacggaaac tagcaccacc ttccacgcgc gtcaacggtg ttcagacgta
     2521 cccaccgcat cgcgggttcc ggcatatctc atcccaagaa aaatctgcgt gaattcttga
     2581 acctagcagg accacgcggt catcgaattt gcttcaggaa gttcgggaag gttagcgaga
     2641 tcggctggga gaccgcgcgg gcaaaaagtc cgcctccgcg gcttgcactg gacatcccca
     2701 tctcactagc gttgcaaatc aatggcagag aattccgaaa acatcggcag cgcactgacc
     2761 ggcgacgccg cggaagcggc actgccccgg ttttcccccg caacgtggcg accctcgttt
     2821 aacccttggc tgatcgccgt tatcgttgct ctcgctgcat tcatggaagt gctcgatacg
     2881 agcattgcca atgttgcgct gccctacatg gccggcaatc tcggcgtcag caacgatgaa
     2941 agcacctggg tgctcacgtc ctatctcgtc tcaaacgcga tcgtcttgcc gatcagtggc
     3001 tggcttgtca ccgtagtcgg acgcaagcgg ctgttcatga cgtgcctctt cattttcacc
     3061 gtgagctccc ttttgtgcgg aatcgcgccc agcctcggtg ccctgattct tttccgcatc
     3121 cttcaaggtg cagggggcgg cgggctccag cctttagcgc aatccatttt ggcggattcg
     3181 tttccgccag aaaagcgggg cattgccttc gccgtttatg gcatcaccgt aatcgtcgcg
     3241 cccaccatcg gtccaactct tggcggatgg atcacggata actacacctg gcgctggatt
     3301 ttcttcatca acttgcccgt cggcatcttg gcgctgctgc tcgtctatca attcatcgag
     3361 gacccgccgt gggcccaccg catcaaggat gctctcgccc gaatcgacta tatcggcatc
     3421 tctctgctga cgttgggcgt gggcgcattg caggtgatgc tggataaagg acaagaagac
     3481 gattggttcg gctcgcgttt cattctcacg tttttcatcc tcgcggcgat tggactggtt
     3541 tcgctattca tctgggagtg gttcgctaaa aacagcgttg tggacgtgcg cctcttcaag
     3601 aacctgaatt ttctcggcgc caacgtgatg atgttcaccc tcggtgtcat ttacttttcg
     3661 agcctcgttc taatgccgca gtacctgcaa actctgatgg gctacaccgc cgaaacctcc
     3721 gggttcgtgc tcaccgccgg ttccgctgtg ttgctcttca ccatgccttt gatcggggtt
     3781 ctttccggga agtttcaggc gcgctacctc atcgcctttg gttggctcag tcttgtattc
     3841 ggcttctatt actccgctgt ccgtctcgac ttgggcatca gcttcggcgt ggccagcgtc
     3901 attcgagtcg ttcaggttat tgggctgccc ttcttgttcg ttcccatcaa cctggcttcg
     3961 tatgttggca tgccgccgga caaaagcagc agcgtctcag gcctgatcaa cttcatgcgt
     4021 aatattggca gcagcgtcgg cacctcgctg gttacgacac tgatcgcccg ccgcgctcag
     4081 ttccaccagg tgcatctcgt agggaatctt gcttcgggaa acgcgacgct cgcgggcacc
     4141 gcagccgcac tggccgccca cctcacggct tcgggcgtaa acgccgagcg ggccatggag
     4201 caagcatatg ccctgatcta ccgcaccgtg attgcccagg cgagcactct cgcctatatc
     4261 gatacctttt tcgttctgac ggccggagca gcaatcatgt ttctgctttc cttcgctctg
     4321 aagaagaatg accccagcgg gcgccgcgtc gtgatggaat agctgcaata tggtaagctt
     4381 cggctggtcg cacttcacct agctggcgga cgaaaaaccg ccgcgtagaa ggcgcccaac
     4441 gtcgatgatt cgaatcatca ccatcgagcg tgaatacggc agcggcggcg ccgaaatcgc
     4501 caaaagagtc gcggcgcggc tagactggaa gttgtgggac cagttgttga ccaacgaaat
     4561 cgcccgcctg atggattgcg actgccgcgt ggtcgaagaa catgaagaaa agcgagatcc
     4621 tctctactac cgcctattca aatctttcat gcgaggcagc ttcgaaggaa gtctgaacga
     4681 gccgcggctg agaatggcgg atacggattg catccgcgaa gtcaccgagc gcgtcgtcaa
     4741 ggatgtagcg gagcgaggcc aatccgtcat cgtcgggcgc ggctccgcct actatctcca
     4801 gggccgttcc gatgcctttc acgtgttcgt ttacgcctcg cttggcgaaa aggtccagcg
     4861 cttgcgcgcc accggcaaaa gtgaacgcga ggccgtccag cttgccgaaa cggtcgatct
     4921 cgaccgcgcc gccttcatca agcaatactt cggcgtcgaa tggccggacc gccaccgctt
     4981 tcatttgatg gtcaactcca ccattggcac ggacgcggcg gtagacctga tacttgaggg
     5041 taccggcatg caggacaagc ctcaaacggt aagcgcccct ccagccctaa aagtcgaaaa
     5101 ggcatgacaa tcgaaacggc cagaaccacc ccggctcgat cagccaaaaa agcaactccc
     5161 gcaggcaaca actagaaaaa gcacctcaga ttgaaattcc ctgcgcctga atttttcgat
     5221 gatgccgcgg agcctacgct cggaaggtta acaattcgca caactccggt gtcgaagtcg
     5281 gctcaaaccg catctataag aatatactca gccgcgatcg aaaacccagg cattgaagca
     5341 ggcgaaattc gacggcagcg tcgaatccag cctcgtccag caaggtttcg atggaaaagc
     5401 caagcaaaac cgcggatttg gcatgcccct tttgcggaag caggagagt
//
LOCUS       DATNDT010000232         4019 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3165968, whole genome shotgun sequence.
ACCESSION   DATNDT010000232 DATNDT010000000
VERSION     DATNDT010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3165968"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VN788_07475"
     CDS             complement(<1..381)
                     /locus_tag="VN788_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20405.1"
                     /translation="MRFIRISGPLALILAASACLAPHVSAQTKPVAKAKATRKAKAAP
                     AAHVAKARLSAVKPAGAPPAAVRETANRRDPFEPLINQRKAGGGVPLPPGKAGLVIAT
                     MRVDGAVHSPSGMIAVVSNPQNSVY"
     gene            complement(392..1024)
                     /gene="pilO"
                     /locus_tag="VN788_07480"
     CDS             complement(392..1024)
                     /gene="pilO"
                     /locus_tag="VN788_07480"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HXU20406.1"
                     /translation="MATSFREWPWPLQALLYVGVAVVLVLVGLYVPGLPLAGVRSQLE
                     TAQSQLKPLESEVQSLRVYKQRRAELESEMDALQKQLATLQAIVPEEKEADQFILMVQ
                     RSALGSGVAIRTLTAKPVAQKPYYFEMPFEIQADGPYYGVLDFFSSLGRLSRIINVGD
                     LKLTSVGQHQGTGKFHVVPGTSVTGTFTITTFFSNSSAQPAPPAKARAKR"
     gene            complement(1027..1662)
                     /locus_tag="VN788_07485"
     CDS             complement(1027..1662)
                     /locus_tag="VN788_07485"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HXU20407.1"
                     /translation="MIRINLLGQARPKAAKQAVPLESTLQVVLGVAAIAVALVVLSVV
                     YFSQKRQLEDTTRKIQQLQAEKAGLQQIKQDVDRFENQKTVLQQRIDMIETLQKNRSG
                     GQDLLRMVANTVVRVDSVWLTSMSRTAGALEIDGEAGSINSVANFITELKRSGYFDKI
                     EIKDAKENDLVPGVETYSFSMTAAVAGASGAPKAETTAATGAARPAAKGRS"
     gene            complement(1659..2711)
                     /gene="pilM"
                     /locus_tag="VN788_07490"
     CDS             complement(1659..2711)
                     /gene="pilM"
                     /locus_tag="VN788_07490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HXU20408.1"
                     /translation="MFNLGKTRSLIGLDIGSSSVKAVELKKVKDGYELVSFGLEPLSQ
                     DTVVDGAIMDAPSVAEKIIAIFDTQKIKVKDVATSVSGHSVIVKRVSMPLMTEDELYD
                     RIQAEASQHIPFDIADVNLSYQLLEATENQMDVLLVAVKKDKILNHTNVLAQAGKTPV
                     VVDIDAFALQNSFEVNYDPDPSQVVALLNVGASVMNINIIRGWTPLFTRDVSVGGNQY
                     TDALQKELDLSFEDAEKLKMGGTVAGVSDEQRTAILRSVSDILILEIQKTFDFFRATA
                     SGENIRRIVVAGGTARVPGLLDLLKEEFAMPVEELYPLRKIAVDPAHHDESHIRDLAP
                     RLAIAVGLALRSFDTP"
     gene            complement(3095..>4019)
                     /locus_tag="VN788_07495"
     CDS             complement(3095..>4019)
                     /locus_tag="VN788_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HXU20409.1"
                     /translation="RTTGMHWQTQKHGAEHFRSARSKNAGGRLEVAVAIGADPATCLA
                     GILPVPPDMDEFLFAGFLRRDPVELVACKTVDLEVPANAEIVLEGHVELGELRTEGPF
                     GDHTGFYSLEGQYPVFHVTCITHRKDPLYLTTIVGPPPQEDYFIGHAIERVFLPVMKM
                     QHPEIVDVAMPAEGIFQNLMIVSIRKSYPGHARKTMNAIWSLGQAMFTKVIVVVDHDV
                     NVQDSREVVWKALCAIDPERDIQFVLGPVDTLDHAARRQDFGSKMGIDATRKWPEEGF
                     SGRWPDEIKMDAATKRRIDELWSHLKLDSRK"
BASE COUNT          812 a         1190 c         1111 g          906 t
ORIGIN      
        1 ataaacactg ttctgcggat tcgaaaccac ggcaatcatt cccgaaggag aatgcacggc
       61 gccatccacg cgcatcgtgg caatcacaag gcccgctttt ccggggggaa gaggcacacc
      121 gccgcccgct ttcctctggt taatgagcgg ctcaaaaggg tcccgcctat ttgcggtctc
      181 gcgtacagca gcaggcggcg cacccgccgg ctttacggcc gacaggcgag ccttcgccac
      241 gtgtgcggcc ggcgctgctt ttgctttgcg tgttgccttc gcttttgcaa ccggcttggt
      301 ctgcgcactt acgtgcggtg ccaggcaggc tgaagccgca agtatcagcg ccagcggtcc
      361 ggatattctt atgaatctca tcgtcatagc cctaccgttt tgcccgtgct ttagccggag
      421 gtgccggctg tgcagatgaa ttgctgaaaa aagtggtaat agtgaatgtt ccggtcaccg
      481 atgttcccgg taccacgtga aactttccgg tcccctggtg ctgccctacg gaggtgagct
      541 tcagatctcc cacattgata attcgggaga gtcgcccgag gctggagaaa aagtccagca
      601 ctccgtagta ggggccgtcg gcctgtattt cgaatggcat ctcgaagtaa tacggctttt
      661 gagcaacggg cttcgctgtt aaggtgcgaa tggcaacacc ggaacccaag gccgaccgct
      721 gcaccatcaa aatgaattgg tcggcttctt tctcttccgg cacgatggcc tgcagagtgg
      781 cgagctgttt ttgcagcgcg tccatttcgg attccaactc ggcgcggcgt tgtttgtaaa
      841 ctcgcaagct ctgcacttcg gactcaaggg gcttcaattg gctttgcgct gtctcgagct
      901 gactgcggac gcccgccaga ggcagtcccg gcacatacaa gcccaccaac accagcacga
      961 cggctactcc aacataaagg agcgcctgta agggccaggg ccattcgcgg aaactcgttg
     1021 ccatggtcag ctcctcccct tagccgcggg ccgggcagcg cccgttgccg cggtcgtttc
     1081 agctttgggc gcgccgctcg cgccggcaac ggcggcggtc attgagaagc tataggtctc
     1141 cactccggga acgaggtcgt tctctttggc gtctttgatt tcgatcttgt caaaatagcc
     1201 cgagcgcttc agttcggtga taaagttggc gacggagttg atcgatcccg cctcaccatc
     1261 gatttccagg gcgcccgcgg tcctcgacat ggacgtcagc caaacggaat cgactcgcac
     1321 caccgtattg gcgaccatcc gaagcagatc ctggccgccg ctgcgattct tttgcagtgt
     1381 ctcaatcatg tcgatgcgct gttgcaaaac ggttttttga ttttcaaaac gatccacgtc
     1441 ctgcttaatc tgctgcaggc cggccttctc ggcttgcaac tgttgaattt ttcgggtggt
     1501 gtcttccagt tgccgcttct gcgagaagta gaccaccgac agaaccacga gggccaccgc
     1561 tatcgcagcc actcccagaa cgacttgcag agtggactcc agtggcacgg cttgtttcgc
     1621 cgccttagga cgagcttggc cgagtaagtt gatccgaatc atggcgtgtc aaaactcctc
     1681 agcgccagcc ctacggcgat tgccaggcgc ggcgccaaat ctcgaatgtg gctttcatcg
     1741 tggtgcgcag gatcgacggc gatttttcgc agtgggtaga gttcttccac cggcatggcg
     1801 aactcctctt tcagcagatc gagcaagccg ggaacgcgtg cggtcccccc agccaccacg
     1861 atccggcgga tgttttcgcc gctggccgtg gcgcggaaga aatcgaaggt cttctgaatt
     1921 tccaaaatca gaatgtcgga aaccgaacgc aagattgcgg tccgctgctc atcgctcacg
     1981 cccgccacgg tgccgcccat cttcagcttc tcggcatcct caaaactcag atccaattcc
     2041 ttctggagag cgtccgtgta ctgattgcct cccaccgaaa cgtcgcgggt aaagagcggc
     2101 gtccagccgc gaatgatgtt gatgttcatc acgcttgcgc ccacgttcag cagggcaacc
     2161 acttgcgaag ggtccggatc gtaattcact tcgaagctgt tctgcagggc gaaggcgtca
     2221 atatccacca ccacgggcgt tttgccggcc tgtgccagca cgttcgtatg attcagaatt
     2281 ttgtcttttt tcaccgcgac cagcagaacg tccatctgat tctcggtcgc ttccagcagc
     2341 tggtagctca aattgacgtc cgcaatatcg aaggggatat gctggctcgc ttccgcctgg
     2401 atccggtcgt acagctcgtc ctcggtcatc aacggcatgc tgacgcgctt aacgatgacc
     2461 gagtggccgg aaaccgaagt ggccacgtct ttcactttga ttttttgagt gtcgaagatc
     2521 gcgataatct tttccgcgac cgatggcgcg tccatgatgg cgccgtccac cacggtgtcc
     2581 tgggacaaag gctccaagcc gaaactcacg agttcgtagc cgtcttttac cttcttcaac
     2641 tcgacggcct tcaccgaact ggatccgata tccaggccaa tcagagatcg cgtcttgccc
     2701 aggttaaaca tcttgcggcc cccgcatcct catcgcaccc cgttgcgcaa gggtgatctc
     2761 ggcagagcta ttcatgaccc ctgctttccg gaaaggcttc acttttcatt cgtcgcgttt
     2821 ggccccgaac cccgtaaggg ccctttttgt cagcgcacac ggtagtactg ataatcctgg
     2881 tacgctgcct tgtttcgttt attgcatccg ggattcacag tgtcaaccaa catgttgccc
     2941 gcgtgtgctt ttggtaaaac ccggccgaaa gaacagtgaa acagcaaata gggcctaaaa
     3001 cattgaggaa ttcttcgaaa agggaggccc aagcgaggag ggcaatgcgg cggaagcgtt
     3061 aatagcaggc agcgccaaat tttgctctgt agggctattt tcggctatcg agtttgaggt
     3121 gggaccacaa ttcgtcgatg cgccgcttgg tcgccgcatc cattttaatt tcgtcgggcc
     3181 agcgaccgga aaatccttct tcgggccatt tgcgagttgc gtcgatgccc atcttcgatc
     3241 cgaagtcctg ccgccttgcg gcgtgatcga gagtatcgac cggacccagt acaaactgaa
     3301 tgtcgcgttc cgggtcgatc gcgcagagcg ccttccaaac cacctcgcgc gagtcctgca
     3361 cgttgacatc gtggtcgacg accacgatta ccttggtaaa catggcttgc ccgagcgacc
     3421 agatggcgtt catcgtcttt cgcgcatggc ccgggtagct cttgcgaatc gagacgatca
     3481 tcaaattttg gaaaattcct tcggcgggca tggccacgtc cacgatttcg gggtgctgca
     3541 tcttcattac cggcaggaag acgcgctcaa tcgcgtggcc gataaaataa tcttcctggg
     3601 gcggcggccc gacgatggtc gtcaggtaga gcggatcctt gcgatgcgtg atgcaggtga
     3661 cgtggaaaac cgggtattgc ccctcgagcg aataaaatcc ggtatggtcg ccaaaagggc
     3721 cttcggtgcg caattctcca agctcaacat ggccctcgag aacgatttcg gcattggcag
     3781 gcacttcgag atcgacagtt ttgcacgcta ccaactcgac gggatcgcgc cgcaggaatc
     3841 cggcaaacaa aaactcgtcc atatcgggag gcacgggcaa aatgcccgcg aggcacgtag
     3901 ccggatcggc tccaatcgca acggccactt ccaacctgcc gccggcattc ttcgaccgcg
     3961 cgctgcgaaa atgttccgcg ccgtgctttt gcgtctgcca gtgcatgccg gtggtgcgc
//
LOCUS       DATNDT010000233         2065 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2920052, whole genome shotgun sequence.
ACCESSION   DATNDT010000233 DATNDT010000000
VERSION     DATNDT010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2920052"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..482)
                     /locus_tag="VN788_07500"
     CDS             complement(9..482)
                     /locus_tag="VN788_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20410.1"
                     /translation="MERKYRQRGYSDRDAAEKKRERAERRDHPGEPRPKQDMLGPRTP
                     RMVGTVTRARCSSCGAVLAPSVDPNGECPRCHFELHSCKQCVHFDTGKQFECTQPIPE
                     RIAKKDAKNNCTFYELRVTVEKDTSPVTYAKSAPAASAAARPNDARQAFEDLFKK"
     gene            complement(604..>2065)
                     /gene="hpnE"
                     /locus_tag="VN788_07505"
     CDS             complement(604..>2065)
                     /gene="hpnE"
                     /locus_tag="VN788_07505"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HXU20411.1"
                     /translation="MPSQSVLVIGGGLAGLASAVALAEAGIEVQLFEKRPHLGGRATS
                     YTLPDGSEVDNCQHVTLGCCTNLDDFYRRAGSAGKIRFYNRLYFADRQGRRFTMDASW
                     LPAPLHMAPSFLTFGALGFADKRAIGRAMFAITWARGRPPGATGASMLDWLRRMKQTP
                     VAIARFWRVVLVSALDEELSRMDAQYGIDVFWKAFLANRRGYRIGIPSVPLGELYEGA
                     RGAIVARGGRVRTRAGVREIRMCDGKFACAVLEDGSEISANACISAVPHSVLMDMVPG
                     RLGEVDGPLAGLKNIRTSPITGVHLWFDRPVMKEPFLALLEHATQWIFNKSLLYAGSK
                     SEKGTEIGSGRGEAGAAEQYLQLVISASYDLVPRSRQEIIDLCLRELADVLPLTREAK
                     LEKATVIKEIHATFSPEPGVNRWRPSQKIGVENLYLAGDWTQTGWPATMEGAVRSGYL
                     AAEAALAKFGGPRRYLQPDLPLEGLSALRATRASVE"
BASE COUNT          421 a          647 c          581 g          416 t
ORIGIN      
        1 aggaaagact acttcttgaa gagatcctca aaagcctggc gtgcatcgtt cggtctcgcg
       61 gcggcggagg cggccggagc actcttggcg taggttacgg gcgaagtgtc cttttcgacg
      121 gtcacgcgca actcgtaaaa ggtgcaattg tttttcgcat ccttttttgc aatgcgctcc
      181 ggaatcggct gcgtgcactc gaactgtttt ccggtatcga aatgaacgca ctgcttgcac
      241 gagtgcaact cgaaatggca gcgcggacac tcgccgttgg ggtccacact cggtgcaaga
      301 acagctccgc agctcgaaca acgcgcacga gtcaccgttc cgaccatgcg cggcgtgcgg
      361 ggcccgagca tgtcttgctt ggggcgaggt tcgccgggat gatcgcggcg ctccgcgcgc
      421 tcgcgtttct tttctgcggc atcgcggtca gagtatcccc gctgcctata cttccgctcc
      481 atgaaatcct cctgggggtt atcccatgat aacccggaaa acatccaagc cgaacgccaa
      541 cttcacgctc gtgcttcgag aatggcacac gcgcggatca cgactggtac tcggacatta
      601 ccgttattcg accgacgctc gcgtggcccg caaagcggac aggccctcca acggcaaatc
      661 cggttgtaga tatctgcgcg gccctccaaa cttggcgagc gcagcttccg ccgcgagata
      721 gccgctgcga acagcgcctt ccattgtcgc gggccagccc gtctgcgtcc aatcgccagc
      781 aagatacaaa ttctcgacac cgattttttg gctgggtcgc caacgattca cacccggttc
      841 cggcgaaaac gtggcgtgaa tttccttgat tacggtagct ttctcaagct tcgcttcgcg
      901 cgttagcgga aggacatccg ccagttctcg aaggcacagg tcgatgattt cttgcctcga
      961 gcgcggcacc aagtcgtatg acgcgctgat gacaagctgc aagtattgct ctgcagcgcc
     1021 ggcttcacct ctgccggatc cgatttcggt ccccttttca ctcttacttc ccgcgtacag
     1081 gagcgactta ttaaaaatcc attgcgtggc gtgctcgagc aaggctagaa acggctcctt
     1141 catcaccggg cggtcgaacc agaggtggac accggtgatg ggagatgttc ggatattctt
     1201 cagtcctgct aaaggcccat ccacttcgcc cagtcgcccg ggcaccatat ccattaagac
     1261 gctgtggggg accgccgaga tacaagcatt tgccgaaatt tccgagccat cctccagcac
     1321 agcgcatgcg aatttgccgt cgcacatgcg aatttcgcgg acgccagccc gtgtccgcac
     1381 gcgcccccca cgagccacta tggctcctcg ggcaccttcg taaagctctc cgagcgggac
     1441 tgagggaata ccgatacgat atccgcggcg gttggcgagg aacgccttcc agaaaacgtc
     1501 aatgccatat tgcgcgtcca tgcggctgag ttcctcatct agcgcgctaa ccaggacgac
     1561 gcgccagaag cgggcgatcg ctactggcgt ttgcttcata cggcgcaacc aatcgagcat
     1621 cgaggcgccc gtcgcgcctg gcggcctgcc gcgagcccac gtaatcgcga acatcgccct
     1681 gccaatcgcc ctcttgtctg caaaacctaa ggcaccgaat gttaggaacg acggcgccat
     1741 gtgcaagggc gcaggcagcc atgaagcgtc catcgtgaag cggcggccct gtcggtcggc
     1801 gaagtacagc cggttgtaaa agcgaatctt cccggccgat ccggcgcggc ggtaaaaatc
     1861 gtccaaattc gtacagcagc cgagcgtaac atgctggcaa ttgtccactt cgctgccgtc
     1921 tgggagcgtg taagaagtgg cgcgccctcc taagtgcggg cgcttttcga acaattgaac
     1981 ctcgataccg gcttcagcca gcgcgacggc ggaggctaat cccgccagac cgccgccgat
     2041 caccagtacg ctttgcgagg gcatc
//
LOCUS       DATNDT010000234         2902 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2316879, whole genome shotgun sequence.
ACCESSION   DATNDT010000234 DATNDT010000000
VERSION     DATNDT010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2316879"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            42..929
                     /locus_tag="VN788_07510"
     CDS             42..929
                     /locus_tag="VN788_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20412.1"
                     /translation="MVRFWLGRFFLGFACLSSGLLLISPRAAAQGTPYFVTYSHHLEE
                     PGNLEIEAYSIYGTQKGAGGFIAPWMEFEYGVTAWWTTELYIDSQHTFGDSSVFTGAR
                     WENRFRPLMREHWINPVLYVEFENTTDADKTLKEVVGFDSQFDAAEPNLKANASHAHE
                     IETKLILSSDYKGWNFSENFIGEKNLGHEPWEFGYALGVDRPLRLAATPRPCNFCREN
                     MIVGAEMYGGLGTVKAFTLSGTSHYVAPLVAWQLPNGVTFSLSPGFGLNDNSHRFLLR
                     WGMSYEIAAFGRRIRSLLQ"
     gene            926..1327
                     /locus_tag="VN788_07515"
     CDS             926..1327
                     /locus_tag="VN788_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU20413.1"
                     /translation="MSRLVISAALVVISLATAGAAARNRGVDRRGVLEAIAQAPAKAR
                     AWKNPYDNQPSAIAAGAKLYRQHCAECHGEDARGIGRAADLHSTGVQNATPGEIAWFL
                     RNGNLASGMPSWSGLPEARRWQIVAYLKTLD"
     gene            complement(1453..1953)
                     /locus_tag="VN788_07520"
     CDS             complement(1453..1953)
                     /locus_tag="VN788_07520"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheX"
                     /protein_id="HXU20414.1"
                     /translation="MKMDLIQPFIGSLDTVLAEMMKAPATIADLAMEEDGYRRKGVAA
                     VVTFKGQIEGRIVLDMDSQAAAKVASCLAGTEVDAAEQIVSEAVCELANMVIGNAVTQ
                     LNDRGFTFKVFPPAVLTEEQCAKAGQDSEATILCFETASGNVYLNVAMHYHARRSREH
                     SSVSVA"
     gene            complement(1950..2396)
                     /locus_tag="VN788_07525"
     CDS             complement(1950..2396)
                     /locus_tag="VN788_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU20415.1"
                     /translation="MKQFEALTRKDSQPVRYLIVDDSVFARKSLAKMVESFGGHLAGE
                     AGDGCTAITEYARTKPDIVLMDITMPQMEGIEAVERIVQEHPEARVVMVSSVGYQDNI
                     LAALQKGARHFVQKPVKPDALYEIIRYVLNDDSVATPVGAAGESSR"
     gene            complement(2393..>2902)
                     /locus_tag="VN788_07530"
     CDS             complement(2393..>2902)
                     /locus_tag="VN788_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CheB methylesterase domain-containing protein"
                     /protein_id="HXU20416.1"
                     /translation="LNESSALEVKEAQSGDLLLAGRALICPGNRHMKVRRMDHGDVAI
                     LVDQPRVNGHRPSVDVLFHSVATEFGDKALAVLMTGMGEDGAAGLGAIHSAGGFTIAQ
                     SPDTCVVDSMPRAAIERGFASRVISLQGLASALQSKCMPQAPSAEPDPVASSSDQPSG
                     AGASRRRSP"
BASE COUNT          585 a          824 c          868 g          625 t
ORIGIN      
        1 tccagttgat actggctatc agttaggaag gatgctgttt cgtggttcgg ttttggttgg
       61 gccgtttctt tctcggattc gcttgtctat cgagcggatt gctcctgatt tcgccgcgtg
      121 ccgctgcgca agggactcca tacttcgtga cctactccca tcatctggag gagcctggaa
      181 atctcgaaat tgaggcctat tcgatttacg ggactcagaa gggcgctgga ggtttcatcg
      241 caccctggat ggaattcgag tacggcgtga ccgcctggtg gacgaccgag ctctatatcg
      301 actcgcagca tacgttcggc gacagcagcg tttttaccgg ggcgcgatgg gagaaccgat
      361 tccggccgct gatgcgcgag cactggatca acccggtgct ctacgtagaa ttcgaaaaca
      421 ccactgatgc ggacaagacg ctaaaggaag tcgtcggctt cgactcacag ttcgacgccg
      481 ccgagccaaa cctcaaagca aacgcctcgc acgcgcacga gatcgaaacc aaactgatcc
      541 tttccagcga ttacaaaggc tggaacttct ccgagaattt catcggcgaa aaaaatcttg
      601 gccacgagcc ctgggaattc ggttatgcgc tcggcgtgga ccggccgctg cgccttgcgg
      661 ctacgccgcg gccctgcaat ttctgccgcg agaacatgat cgtcggcgcc gaaatgtatg
      721 gcggactggg cacggtgaag gccttcacac tatccggcac atcgcactat gtggctccgc
      781 tggtggcctg gcagcttccg aatggtgtca cattctcgct ctcacccggt ttcggcctga
      841 atgacaatag ccaccgcttc ctgttgcgct ggggcatgtc gtacgaaatc gcggctttcg
      901 ggcgcaggat taggagctta ttacaatgag ccgtctggtg atttcggccg cactcgtggt
      961 catttcgctg gcaacggctg gggctgccgc tcgcaaccga ggcgttgatc gccgtggcgt
     1021 tctggaggca attgcgcagg cgcccgcaaa ggctcgagcg tggaaaaatc cttacgacaa
     1081 tcaaccaagt gctatcgccg caggcgccaa gctctaccgg cagcattgcg cggagtgtca
     1141 cggcgaggac gcgaggggta tcggtcgcgc cgcggacttg cactccactg gagtgcagaa
     1201 tgctacgccc ggcgagatcg cttggttttt gcgaaatggg aaccttgctt ccgggatgcc
     1261 ctcgtggtcc gggttgccgg aggcgcgccg gtggcagatt gtggcgtacc tcaaaacgct
     1321 ggattagtgt tgcgtccctg aaattctgga tagatgagct tcgacacgga agctgtacct
     1381 caggggctaa agcccttttt ctcccgggac ttgagcggca cggttgaaac cgtgccctga
     1441 cgagttcagc gtctacgcga cagaaacgga ggagtgctcg cgggagcgtc gcgcgtggta
     1501 atgcatagcg acattcaggt atacgtttcc gctggcagtt tcaaaacaga gaattgtggc
     1561 ttccgaatct tgacctgctt tggcgcactg ctcttcagtg aggaccgcag gagggaagac
     1621 cttaaacgtg aacccgcgat catttaactg ggtgacggcg ttgccaatca ccatgttcgc
     1681 cagctcgcaa acagcttcgg aaacgatctg ctcggcagca tccacttcag ttcctgcgag
     1741 gcagctggca actttggcgg cggcttgcga gtccatgtcc aggacgatcc gaccttcgat
     1801 ctggcctttg aaagtgacca ccgcggcgac acctttcctg cggtagccgt cttcctccat
     1861 cgcaaggtcc gcgatggtgg ccggagcttt catcatttcg gcaaggaccg tatcgagcga
     1921 gccgatgaac ggttggatca gatccatttt catcgcgaac tctcccctgc cgcgccgacg
     1981 ggagtggcaa cgctatcgtc attgagcacg taacggataa tttcgtagag cgcgtcgggc
     2041 ttcaccggct tttgtacgaa gtggcgcgcg cccttttgaa gcgcggccag aatattatcc
     2101 tgatagccga cggaagagac catgacgaca cgcgcttccg gatgttcctg aacgatgcgc
     2161 tcgacggcct caatgccttc catttgcggc atggtgatat ccatcaggac gatatcgggc
     2221 ttcgtgcggg catattcggt gatcgcggtg cagccatcgc cagcctcgcc ggcgagatgg
     2281 ccgccgaaag actcgaccat tttggcgagg ctctttctgg cgaagaccga gtcgtccaca
     2341 atgagataac gaacgggctg gctatctttt cgggtcaacg cttcaaattg tttcatggtg
     2401 atctccttcg ggaagctcct gcgccggacg gttgatctga agacgaggcc accgggtccg
     2461 gctccgcgga tggcgcttgg ggcatgcact tcgattgcaa ggcgctggca aggccttgaa
     2521 gcgaaataac tcggctcgca aagccccgct caatggccgc gcgaggcatg ctatcgacga
     2581 cgcaagtatc cgggctttgg gcgatcgtaa agccaccggc agaatgaatc gcgccaagtc
     2641 cagcggcacc atcttctccc attcctgtca tcagcacggc caaagccttg tcgccaaatt
     2701 cggtggcgac agaatggaag aggacatcga cggaggggcg gtgcccgttg acgcgcggct
     2761 gatccacaag gatcgctacg tccccgtgat ccatgcggcg cactttcatg tgacggttgc
     2821 cggggcagat cagggcgcgt ccggcgagga gcaaatcacc tgattgcgct tccttgactt
     2881 cgagggccga ggattcattc aa
//
LOCUS       DATNDT010000235         5537 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2901315, whole genome shotgun sequence.
ACCESSION   DATNDT010000235 DATNDT010000000
VERSION     DATNDT010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5537)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5537)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5537
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2901315"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /locus_tag="VN788_07535"
     CDS             complement(<1..995)
                     /locus_tag="VN788_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HXU20417.1"
                     /translation="MVEESFKTSSGKSRKRIAVIPGDGVGKEVIPEAVKVLKAAAAAC
                     GREIHLTEFDWGADRFLRDGTTLAVDAPEMFRREHDAILLGALGDPRVPSNQHGADIL
                     LGLRFKLDLYVNLRPCILFDERLTPLKNRTEKDVNICVIRENTEGLYSGVGGFFKKGT
                     ADEIATQEDINTRKGVERIIRYAFEFAVARGYKRVCMSDKSNALAFGHDLWQRVFKEV
                     RSEYPGIESSHMFIDNLLMQMVRDPSQFQVIVTCNLFGDIASDVGAGLVGGLGVTPSG
                     NIHPGRVSLFEPVHGSAPDIAGKNIANPIGAVLAAAMMCEYLGWNDVACGMEEGVR"
     gene            1145..1663
                     /locus_tag="VN788_07540"
     CDS             1145..1663
                     /locus_tag="VN788_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU20418.1"
                     /translation="MNQNRPEEPWLRGTLADVAPVPRAVLHALELAKEDIERWCDGLS
                     DDQFNSRPFGIASLAFHIRHIARSMDRLLTYADGRSLTDEQLAALKTELDEGAARSEL
                     FAELDAAFEKSAERIRALDATRLSDTREVGRRRRPTTVGGLLIHVAEHTQRHVGQAIT
                     TAKIVTAQHQSE"
     gene            complement(1664..2980)
                     /gene="aceA"
                     /locus_tag="VN788_07545"
     CDS             complement(1664..2980)
                     /gene="aceA"
                     /locus_tag="VN788_07545"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003354103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HXU20419.1"
                     /translation="MQNGKPQHLEMEWKTNPRWAGITRPYSEKDVAQLRGSVHIEHSL
                     ARLGAERLWNLLHSQDFVAALGAMTGNQAVQQVAAGLKAIYVSGWQVAADANDAAETY
                     PDQSLYPANSVPNLVRRLNHSLMRADQIHHAEGHNETYWLAPLVADAEAGFGGNLNAF
                     ELMKAMIEAGAAGVHFEDQLSSAKKCGHLGGKVLVSTLAAIQKLVAARLAADVLGVPT
                     IIVARTDAESATIVTTDSDPRDQAFVTGERTSEGFYRVRSGTKAAIARAISYAPYADL
                     IWCETSTPDLNEAREFADGVHAKFPGKMLAYNCSPSFNWRKNLDDATIAGFQRDLAAL
                     GYKFQFITLAGFHALNHSMYELARGFKQNGMTAYSDLQDREFESEHNHGYEAVKHQRF
                     VGTGYFDAVQNAISSGTASTIALEGSTEAAQFAPRKKRRTESAEAD"
     gene            3654..4313
                     /locus_tag="VN788_07550"
     CDS             3654..4313
                     /locus_tag="VN788_07550"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006963949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HXU20420.1"
                     /translation="MYYEPGKTQHGLPYDPFKSCVVPRPIGWISTISRDGVPNLAPFS
                     QFQNLTFDPPYVMFASNQTTKGRRKDTVVNTEQTREFVWNMATYELREAVNKSAEEVP
                     SEVDEFDLAGVTKIPSHHVKPFRVAESPVHFECAYHQTIHLPGNGTMGTVDIVIGRVL
                     GIHINDAVIRPDGKLDILKIRPIARLGYHDYTSVESIFSMTIPGNDPRRLAGMEGSAR
                     F"
     gene            complement(4449..>5537)
                     /locus_tag="VN788_07555"
     CDS             complement(4449..>5537)
                     /locus_tag="VN788_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU20421.1"
                     /translation="DEPTNHLDLDSVHWLQDYLCEYEGVLIVISHDRHFLNSVCTHTA
                     DIDYQTVIMYNGGYDDMVVAKTSIRSRIEAENAQRERKIAQLNEFIARFSAGTRSAQA
                     TSRKKEVERLQSAELARSNIARPFIRFQLDQHSGRHPLEIKSIVKSYDGKRVFQPFTA
                     SVERGEKIALMGRNGTGKTTLLKSLIRNATGFIEDVDKEFPVDGGTVIWGHNAAVGYF
                     AQDHSESIIKGTTVIEWLHQFKPSAAQEELRGLLGQMLFSGDDALKQTQALSGGESAR
                     LIFCRLMLQKPNILVLDEPTNHLDLESINALNISLQKYLGTVLLVTHDHDVIDEVATR
                     IWHFEGGRIEDFKGPYSEYQVYAEEKVS"
BASE COUNT         1166 a         1670 c         1487 g         1214 t
ORIGIN      
        1 cgaaccccct cttccatccc gcacgcgaca tcgttccagc cgagatactc gcacatcatc
       61 gcggcggcga ggaccgcgcc tatcgggttc gcgatgttct tgccggcgat gtccggggcc
      121 gagccgtgca ccggctcaaa aagtgaaacc cgaccggggt gaatgttccc cgatggagtc
      181 acgccaaggc cgccaaccag ccctgccccc acgtcgctgg cgatatctcc aaaaagatta
      241 caagtaacga tcacctgaaa ctgcgacgga tcacgaacca tttgcatcag caggttatct
      301 atgaacatat ggctcgattc gatgcccggg tactcactgc gcacttcttt gaaaacgcgc
      361 tgccaaagat cgtggccgaa ggcgagcgcg ttggatttgt cactcatgca gacgcgcttg
      421 taacctcggg cgacggcaaa ttcgaatgca tagcggatga tccgctcaac gcctttgcgg
      481 gtgttgatgt cttcctgagt cgcgatttcg tctgcggttc cttttttgaa aaatccgccc
      541 acgcccgagt aaagtccctc ggtgttttcc cgaatcacgc agatgttgac atctttttct
      601 gtgcggtttt tcagcggcgt aagtcgctca tcgaaaagaa tgcagggtcg cagattgacg
      661 taaagatcga gcttgaagcg caggcccagc agaatgtcgg ctccgtgctg gttggatggc
      721 acgcgcgggt cgccaagcgc acccagcaga atcgcatcat gctcccgccg gaacatctct
      781 ggcgcatcga cggccagcgt cgtcccgtcg cgcaggaaac gatcggcgcc ccaatcaaat
      841 tcggtgaggt ggatttcccg tccgcatgcc gcagcggcgg ctttcagcac tttcactgct
      901 tccgggatca cctctttgcc cacgccgtcg ccgggaatca cggcgatccg tttgcgcgac
      961 ttcccggagg atgttttgaa actttcttcg accatccgcg cggaacgtag cacgcgagcc
     1021 aaggagtgtc aaacgtcagc ttagtgcccg cacgcggcag cttgccgtta cccacgcatt
     1081 agtatgatcg ggagttcgcg cgtctggcaa aacgcagtta agctaactgg caggagaaca
     1141 ttccatgaac cagaatcgac cggaagagcc ttggctgcgc ggaacactcg ccgatgttgc
     1201 cccggtgcca agagccgtgc tgcacgcact cgagctggcg aaagaggata tcgaacgttg
     1261 gtgcgatggc ctaagcgatg atcaattcaa tagccggcct ttcggaatcg catcgctcgc
     1321 gttccacatc cggcacatcg cccgtagcat ggaccggctc ctcacttacg ctgatggaag
     1381 gtccttaaca gacgagcagc ttgccgcgtt gaagaccgaa ctcgacgagg gtgctgcccg
     1441 aagcgaactc tttgccgaac tcgacgcggc cttcgagaag agcgccgaaa gaattcgcgc
     1501 cctcgatgcg actcgactaa gtgacacgag ggaggtgggc agacggcgcc ggcccaccac
     1561 ggttggcggt ctcctcatcc acgtggccga gcatactcag cgccacgtgg gccaggcaat
     1621 aacgaccgcc aagatcgtca ccgcgcaaca tcaatcggaa taattagtcg gcttcggcgg
     1681 attccgtgcg gcgctttttg cgcggcgcga actgggcagc ttccgtcgag ccttccagcg
     1741 caatcgtcga cgccgttccg ctgctaatcg cgttctggac cgcgtcgaag taaccggtgc
     1801 cgacgaaccg ctgatgcttc acggcttcgt acccgtgatt gtgctcgctt tcaaactcgc
     1861 ggtcttgcaa gtcggagtag gccgtcatgc cgttctgctt gaatccgcgc gccaactcat
     1921 acatcgagtg attgagtgcg tgaaagcctg ccagcgtgat gaattggaac ttgtacccga
     1981 gagcggccag atcccgctga aagcctgcga tcgtcgcgtc gtccagattt ttgcgccagt
     2041 taaatgatgg cgaacaattg taggccagca tcttccccgg aaacttcgcg tgtactccat
     2101 cggcaaactc ccgggcctca ttcagatccg gtgtggacgt ttcgcaccaa atcaagtctg
     2161 cgtaaggagc gtaggagatg gcgcgcgcga tagccgcttt ggttccactg cggacgcgat
     2221 agaaaccttc cgatgttcgc tcgccggtaa cgaaggcctg atcgcgtggg tctgaatcgg
     2281 tagtcacgat ggtggcgctc tcagcatcgg tgcgtgcgac gattatcgta ggcacgccga
     2341 gcacatccgc ggcaaggcgt gcggcaacca atttttgaat cgccgcaagt gtagagacca
     2401 gcaccttccc acctagatgt ccgcacttct tcgcggaaga gagctgatcc tcaaaatgaa
     2461 cgcccgcggc tccggcctcg atcatcgcct tcatcaactc gaaagcattt aggtttccgc
     2521 caaatcccgc ttcagcgtcg gccaccagcg gagccaacca gtaggtttcg ttgtggcctt
     2581 cggcatgatg aatctgatcc gcgcgcatca gcgaatgatt caggcggcga accaaattcg
     2641 gtacgctatt cgccggatac aaactttggt ccggataagt ttccgcggca tcgttcgcgt
     2701 cagcggccac ctgccaaccg ctcacataaa tcgcttttag tcctgcggct acctgttgta
     2761 ccgcctgatt ccctgtcatg gcgcccagag ccgcgacaaa atcttgcgag tgcagcagat
     2821 tccacaggcg ttccgcgccc aagcgcgcca ggctatgctc gatgtgaacc gagccgcgaa
     2881 gctgcgcgac atccttctcg ctgtacggac gcgtgattcc cgcccaacgt ggattcgtct
     2941 tccattccat ttccaaatgt tgcggctttc cgttttgcat tctgtccgct cctcgtcagt
     3001 cggatttgca gttctgcgcc cgcgcaatcg cgctcggctg ttcggaggtg gctggagaac
     3061 ttcgaaatta gtgatggaac tctgagaatt cgctggttca gccagcgaag aatcaggcgc
     3121 ccccaaggcc tttggggctc aactcctgga gaaaggcgtc cggcgaggtg cgggctgcgt
     3181 atagcccgca gttgactcga agagtgaaaa atacttccat tttttagtct ctaattcctg
     3241 ccacttttag gaccaatctc tcgtaggtca ctgaaacagt gccacttaag actcccatcc
     3301 accgcccact cgccgccctc tatgtgactc cggcttgcga atggcactta cactgccatt
     3361 ttgttcgtcc aggcggcttt ggtcaaccaa gattatcctg atacaagcta ttcataattt
     3421 ttatagccaa atcggcgcgg ccgcaccgca gcaaaaagtt gcgcggtcat cctgagcgga
     3481 gttgcgcgcg cctctgcctt tcccgcgttt ttcgcgggcg cgcaaacgca gccgaaggac
     3541 ctctcttgga ttgaaaatgt tgccaccagc tcaaatcgaa gagccgttct tcagagacat
     3601 cgcaattccg acgcagttat aatggcgcgc gcatttcccg gagaaaattt cgcatgtact
     3661 acgagcccgg caaaacccaa cacggactgc cctacgatcc ctttaaatct tgcgtggttc
     3721 cgcggccgat cggctggatt tccacgatca gccgcgacgg cgttcccaac ctcgcgccat
     3781 ttagccaatt ccagaatctg accttcgatc ctccctatgt gatgttcgcc tcaaaccaga
     3841 ccactaaggg tcggcgcaag gatacggtcg taaacacaga acaaaccagg gaattcgtgt
     3901 ggaatatggc tacgtacgaa ctgcgtgagg cggtgaacaa gtccgccgag gaagtccctt
     3961 ctgaagttga cgaattcgat ctggcgggcg tcacgaaaat tccatcgcac cacgtaaagc
     4021 ctttccgcgt cgctgagtcc cccgtgcatt ttgaatgcgc ctatcatcaa acaatccact
     4081 tgccgggaaa tggcaccatg ggaacagtcg acatcgtgat cggccgcgtt ctcggaattc
     4141 acatcaacga cgccgtaatt cgccccgacg gaaagctgga cattctgaag atccgtccca
     4201 tcgctcgcct cggttaccat gactacacca gcgtcgaatc tattttctca atgaccattc
     4261 cgggaaacga tccgcgcagg ctcgcaggca tggaaggctc cgcccgcttt tgaaccgttc
     4321 tcgtaggggc acggcacacc gtgcccgctt gccctgagcg atgtggcgaa gggtcaaaca
     4381 tacttggcgt gaatttttgc ggtcaacgga cggtgcgcgg aagaagcata cctgcgggaa
     4441 aaatgcggct aagacacttt ctcttccgcg tacacctgat attccgaata cggtcccttg
     4501 aaatcctcga tccggccgcc ctcaaagtgc cagatgcgcg tggcaacctc atcgatcaca
     4561 tcgtgatcgt gcgtcaccag caaaaccgtc ccgagatatt tctgcagcga aatattcagg
     4621 gcattgatcg attcgaggtc caggtggttg gtcggctcgt ccaacactaa aatattcggc
     4681 ttctgcaaca tcagcctaca aaaaatgagt cgcgccgatt cgccacccga aagggcctgc
     4741 gtctgtttca gtgcatcgtc gccgctgaag agcatctggc ccaggaggcc gcgcaactct
     4801 tcctgcgccg cgctaggttt gaactgatgc agccactcga tcaccgtggt gcctttgatg
     4861 atcgattcgg agtgatcctg cgcgaagtag cccactgccg cgttgtgccc ccagattacc
     4921 gtgccgccgt ccacgggaaa ttccttgtcc acatcctcga tgaatcccgt ggcgttgcga
     4981 atcagcgatt tcagcagcgt ggttttccca gtcccgttgc gtcccatcag cgcaatcttt
     5041 tcgccgcgct ccacgctggc ggtaaacggc tggaacactc gcttgccatc gtacgatttc
     5101 acgatgctct tgatttccag cgggtgccgc ccggagtgct ggtccagttg aaaacgaatg
     5161 aatggccggg cgatattcga tcgggcgagc tcggcgcttt gcagccgttc cacctctttt
     5221 tttcgcgatg tagcctgcgc cgaacgcgtg ccggccgaaa agcgcgcgat gaattcgttt
     5281 agctgggcga tcttgcgttc gcgctgggcg ttttcggctt cgattcgcga tcgaatcgac
     5341 gttttcgcca ccaccatgtc gtcgtacccg ccgttgtaca taatcaccgt ctgataatcg
     5401 atgtcggcgg tgtgcgtgca aacactgttt aggaaatggc ggtcgtgcga aatcacgatc
     5461 agcacgcctt cgtattcgca caaataatcc tgcagccagt ggacggaatc caaatccaaa
     5521 tggttcgtcg gctcgtc
//
LOCUS       DATNDT010000236         2967 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_970117, whole genome shotgun sequence.
ACCESSION   DATNDT010000236 DATNDT010000000
VERSION     DATNDT010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2967
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970117"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1358)
                     /locus_tag="VN788_07560"
     CDS             complement(<1..1358)
                     /locus_tag="VN788_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HXU20422.1"
                     /translation="MPSAIEDYALIGDCETAALVARDGSVDWLCLPRFDSGACFAALL
                     GTPDNGRWQIVPAGKYRVTRRYREKTLILETQFDTDDGTVTLIDFMPIRSKTPEVVRL
                     VRGDRGKVAMRMDFVVRFDYGEAVPWVTRQKDGALFAIAGPNLLALQTPVTLRGEGLK
                     TISDFAVAEGETVGFGLTYGSSFGRPPRCTDLESSLEKTEKWWRKWTSKCSYDGPEAD
                     AVERSLITLKAMTYSPTGGILAAVTTSLPEKFGGKRNWDYRYCWLRDATFSLLALMHA
                     GYREEARHWRDWLLRAAAGSANQLQVMYGVAGERRIPETEVRWLSGYRGASPVRIGNA
                     AAEQLQLDIYGEVSDVLHQARRRTKSKNDSAMGLQRELLKHLQKIWREPDHGIWEFRG
                     QGRQLTHSKVMAWVAFDRAIKTCERFGFEGDLDSWQATRQEIHDDVCSHGFDAELGSF
                     VQS"
     gene            complement(1449..2066)
                     /locus_tag="VN788_07565"
     CDS             complement(1449..2066)
                     /locus_tag="VN788_07565"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU20423.1"
                     /translation="MSDNLPKAVLDSADITSISQLILRERESRDVGRWEQMRECFWPD
                     SLVRVSWFRGTGPDFVTGSIDMARRGIPAKHRLGPVLVKLAGDRAIASLTAIIDLPVK
                     LQNVEGTLSTHARFLYRVERRGEQWRIFGFDAIYMRDELTPSIPGEHISISREEVREF
                     RASYRMLSYYLHSQGYDVDSNLPGEDRADLAEALNRELSSWLTVH"
     gene            complement(2080..>2967)
                     /locus_tag="VN788_07570"
     CDS             complement(2080..>2967)
                     /locus_tag="VN788_07570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU20424.1"
                     /translation="PLVTSLEYCGSFVRREKNSILMACNEPSVAGFVPDRTVVRFEWE
                     TPLPRYWKLPFWLPRFQRARYLFLSENDRGEFLHQHSLIPSDSTFVIPYYVDSQLFKP
                     APSAPATPRVGFAGQWVPRKGIAALLDAWRKVKSQVASAELQLAGGAAMWKTAQPVTG
                     AEQSAASVKSMEDAGLLHSVGGLPRAAMPRFWNSVSIAVVPSLYEPFGLVALEAMACG
                     VPVIASKIGGLKEIVQEGVSGLLVPPGDSIALANSIVYLLSNEQIRLRLGEGARRRAE
                     EFSQARRSSELVTLFQPRN"
BASE COUNT          641 a          957 c          790 g          579 t
ORIGIN      
        1 gactgaacga agctgcccag ttcggcgtca aatccgtggc tgcaaacatc gtcatgaatc
       61 tcttgccgcg tagcctgcca gctatccaaa tccccctcga acccgaatcg ctcgcaggtt
      121 ttgattgccc gatcgaaagc cacccaggcc atcaccttgg aatgggtgag ctgcctcccc
      181 tgcccgcgaa actcccaaat cccgtggtcg ggctcacgcc aaatcttttg aaggtgcttc
      241 aaaagctcgc gttgaagacc catcgccgag tcgttcttcg atttcgtgcg cctgcgagcc
      301 tggtgtagga cgtctgaaac ctctccatag atatccaatt gcaattgctc ggcggcggcg
      361 tttccgattc gcaccgggga tgctccccga taaccggaga gccaccgcac ctccgtctcg
      421 ggtatccggc gctccccggc aaccccgtac atcacctgca attggttggc gctcccggcc
      481 gcggcgcgca gaagccagtc tctccagtgg cgcgcttcct cgcgataacc ggcatgcatt
      541 agcgccagca aactgaaagt tgcgtcgcgc aaccagcagt atcggtaatc ccaatttcgc
      601 ttccctccga acttttcagg caacgacgtg gtcacggccg ccaggatgcc gcccgtcggg
      661 gaatacgtca tcgctttcag agtaatcagc gagcgctcga cggcgtccgc ctccggccca
      721 tcatagctac atttggaagt ccatttgcgc caccatttct ctgtcttctc cagggacgat
      781 tccaagtctg tgcagcgcgg aggtcgtcca aaggaacttc cgtacgtcag gccgaagcca
      841 accgtctctc cctcagcgac cgcaaaatcg ctgatcgtct tcagcccttc tccccgcagc
      901 gtcacaggcg tttgaagcgc cagaaggttc gggccggcaa tcgcgaacag cgccccgtcc
      961 ttctggcgtg tcacccatgg aacggcttcg ccgtaatcga agcgaaccac gaaatccatc
     1021 cgcatcgcca ccttcccgcg atcgccgcgc accaggcgga cgacctcggg cgtcttgctg
     1081 cgaatcggca tgaagtcgat cagcgtcacc gttccatcat cggtatcgaa ctgggtttcg
     1141 aggatgaggg ttttctcgcg gtaacgccgg gttacgcgat actttcctgc gggaacgatt
     1201 tgccagcggc cattgtcggg agttccgagg agcgctgcaa aacatgcccc ggaatcgaat
     1261 cgcggcaaac aaagccagtc gaccgagccg tcgcgcgcca cgagggccgc cgtttcgcaa
     1321 tctccaatga gggcgtagtc ttcgatcgcg gaaggcacag aattattgta agtccaattg
     1381 gttcacaaca cgaagcaaca tcgccgattc gccgcaatca cgctcggcag tcggcgcaat
     1441 cgactatcct aatgaaccgt caaccagctc gaaagttccc ggttgagtgc ttccgccaga
     1501 tccgcgcggt cttctcccgg caaattcgaa tcgacatcgt agccttggct gtgcagatag
     1561 taggagagca ttcgatacga cgccctgaac tctcgcacct cctcgcggct gatagagatg
     1621 tgctcgcctg gaatcgaagg cgtaagctca tcgcgcatgt aaatcgcgtc aaatccaaaa
     1681 atgcgccact gctcaccccg gcgttccacc cggtagagaa aacgcgcgtg cgttgaaagg
     1741 gtgccttcca cgttctgcaa tttcacgggg aggtcgatga tggccgtcaa gctcgcaatc
     1801 gcgcgatctc ccgccaactt taccagcacc ggccccagcc ggtgcttcgc gggaatgccg
     1861 cggcgtgcca tgtcaattga accggtcacg aaatccgggc cggtgccccg aaaccagctt
     1921 acccgcacca gcgagtcagg ccaaaagcac tccctcatct gctcccagcg ccccacgtcg
     1981 cggctctccc gctcgcgcaa aataagttgc gagatagaag taatatccgc gctgtccagc
     2041 accgctttcg gtaaattgtc gctcatatat ggtgccaggc tagttgcgcg gctggaacaa
     2101 agttacgagt tccgagcttc gtcgcgcctg cgaaaactcc tcggctctgc ggcgggcgcc
     2161 ttcgcccagg cgaagccgta tctgttcatt agaaagtaaa tatactatcg aattagcaag
     2221 cgcgattgaa tctccgggcg gaacaagcaa accgctcacc ccttcttgaa cgatttcctt
     2281 taagccccct atcttgctcg cgataacggg gacgccgcaa gccatcgcct ccagcgccac
     2341 cagcccaaaa ggctcgtaca gcgacggcac cacggcgatg ctcaccgaat tccaaaatct
     2401 gggcatcgct gcgcgcggta gtccacccac agaatgcagc agccccgcgt cctccatact
     2461 tttcaccgac gcagccgact gttccgcgcc ggtaacgggt tgagcggtct tccacatcgc
     2521 agcgccaccc gctagttgaa gctccgccga cgcaacctgc gatttcactt tccgccaggc
     2581 gtccagcaat gccgcgattc ctttgcgagg cacccactgg cccgcgaagc cgacgcgcgg
     2641 cgtggcaggc gcgctcgggg ccggtttgaa aagttgagag tcaacgtagt agggaatcac
     2701 aaacgtggaa tccgagggga tcagagaatg ctggtgcaga aattctccgc ggtcattctc
     2761 gctgagaaac aaatagcggg cgcgctgaaa gcgcggtagc caaaaaggca gtttccagta
     2821 gcgcggcagc ggagtctccc attcgaacct aactacagtc ctgtcaggga caaagccggc
     2881 gaccgagggt tcattgcagg ccatcagaat gctatttttt tcgcgtcgca cgaaggaccc
     2941 acaatattcg agagaagtaa cgagagg
//
LOCUS       DATNDT010000237         5762 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1231053, whole genome shotgun sequence.
ACCESSION   DATNDT010000237 DATNDT010000000
VERSION     DATNDT010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5762)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5762)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5762
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1231053"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="VN788_07575"
     CDS             <1..368
                     /locus_tag="VN788_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXU20425.1"
                     /translation="PNRIGMVGFSAGAALTMATTLAGEDAKPAFIGIIYGPLASVKVP
                     ADAPPLFIALAADDPIFGNSGYGLIDSWRAAKRPVEFHLYEQGGHGFGMYPKETTSTG
                     WFDAFVRWLTMHGMVKPAQ"
     gene            complement(525..1484)
                     /locus_tag="VN788_07580"
     CDS             complement(525..1484)
                     /locus_tag="VN788_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20426.1"
                     /translation="MTRRFSISTQANIRLERATRPSETLNYSAIPLLYARANARPYYT
                     FRMTGFQAGNIDAFRSERVVCVVKAGEVMSKAFQIVFAADGSLFITFPYFRHRTGILS
                     SSSIPATGTRQTEVPLEHGGKVTSHLVKYSHHTDGRAHFSQTGKIVTAIKRQSIALDK
                     QHGHIFSLLIQGLHALDAADPLKDFGASPKRTAVNFEVEPPEAVKFVGRWFDVNKMRF
                     SNATPTIGPTLLTLDPDGTQRNARLIANPRANIRHVLAVTCQAIPKLGPAPEVFMFYG
                     GFDPPEIMTDPTKEAGFLAFLYPLSEADKARERLGSVDYNAKA"
     gene            1675..2379
                     /locus_tag="VN788_07585"
     CDS             1675..2379
                     /locus_tag="VN788_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU20427.1"
                     /translation="METSQSNLVATLRRLPLFTDLRESELVLIAERITVKQLSRGEIV
                     FSEGDPCRELLIVRDGRVNLLKTTVNGRRQLISVERAGSSLSETSAFDGHAYPVTAET
                     VEDTTLLRLEADHFRKVCLQHPEMALKIIKVLGHRLRRMSRLIEDLSFSTVRGRLVAY
                     LVHLARDEGRSISQGIELELTENNEQLAARLGTVRELVSRNLGRLHNEGLIQMSRRRV
                     RIPNLDVLAEEIANIR"
     gene            2479..2751
                     /locus_tag="VN788_07590"
     CDS             2479..2751
                     /locus_tag="VN788_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HXU20428.1"
                     /translation="MAYVIAEPCIGAKDTACIDVCPVDAIHPGTNEANAATVAQLYIN
                     PDDCICCGMCVPVCPVGAIYAEEDLAEKWKHYIQINRDWFGSSEHI"
     gene            complement(2996..5422)
                     /locus_tag="VN788_07595"
     CDS             complement(2996..5422)
                     /locus_tag="VN788_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20429.1"
                     /translation="MAGLLQDIRFALRQLRKSPGFAITATAMLAVAICANSTVFSWMD
                     GTMLRPIPGARDAGELVSLQRGERNFSPTPPFSYLDYRDLREQNHTLAGILAYHNDWI
                     ALTGGAQPERVYIANVSANYFDVLGIKPVLGRFFLPEEESRAIPNVVLGYSLWKTRYS
                     KDPAIVGKSIEVARHPVTVIGVAPEGFTGAAPGLRDDLWVTLDPLGTDPWRMTHRDSV
                     WLILIGRLRPGVSRGQAAQDLDTQMNHIVAAYPDQHLGDNRITLDPMWRSPFGANGYM
                     AATLPILLAFAGLVLLLTCANVATLTLVRFVSRRRELAIRQSLGANRMQLVRQMVLEG
                     VALSIVAGAAALLLTSWTSKTFAWFFRASSAPIILNGSMDYNVVIGIAVFSLLAGMLC
                     CALPAWRSSYAPAVEVLKAESASISGGSRNRKLLSALVVTQIALSLPLLLCSGLLLRT
                     LRNLADANPGFEQDHILTASVGLNIAGYSNDEAQLIRHKILDRVSALPGVKVASLTDW
                     IPMTLIQKRNDAYPEGYVPHPHESLLVENAEVGPRYFESLHIPILEGREFTLNDDEKA
                     PSVIIVDQTAARHYWPGEDPLGKRLRIWGSLHTVVGVARNSTHTFVNESPEPMIYMSF
                     FQHPGYETMVQVKTQGNPVDLEPAVENAIHEIDPRLPVFDVRSMRESTHLASTFAVIE
                     STLAGMFALIGLVLAVTGIYGVVTYRTQMRTHEIGVRMALGASRVDVLRLVLLQGAWL
                     TGVGLALGLAFALGLTRLIARLLYGIGANDPFTMASVVMLLGAMSLLACYFPARRAMR
                     RNPVTAIREL"
BASE COUNT         1313 a         1638 c         1579 g         1232 t
ORIGIN      
        1 atcccaaccg catcggcatg gttggctttt ccgccggcgc ggcgctgaca atggcaacta
       61 cacttgccgg cgaggatgcg aagcccgctt tcatcggcat catctacggc cctctagcgt
      121 cggtcaaggt gccagccgac gcgccgccac tgttcatcgc actcgctgcc gacgacccta
      181 ttttcgggaa cagtggctat gggctcatcg atagctggag ggccgcgaag cgccccgtcg
      241 aattccatct ttatgaacag ggtggccatg gattcgggat gtacccgaag gaaactacca
      301 gcaccggttg gttcgatgcc ttcgtgcgct ggctcacgat gcatggcatg gtgaaaccgg
      361 cacaatgagg aagcagcagc gctgctactt cgcttcaata tgggaactaa acgcaaacgc
      421 gtatagatca acctctgtag agcgctgaga cttaccggcg gttttattcc ccacacaacg
      481 aaaaccgcgt tgtgtgcgcc aaccgcccaa gaagagataa ccgatcaggc tttggcattg
      541 taatcgacgc tgcccagccg ttcccttgcc ttgtcggctt ctgaaagtgg gtagagaaac
      601 gccagaaagc cagcttcctt cgtcggatcc gtcattattt cgggcggatc aaagccgcca
      661 taaaacatga acacttcagg agctgggcca agctttggaa ttgcttggca ggttaccgca
      721 agaacatgcc tgatatttgc acgcgggtta gcgatgagac gagcgtttct ttgagtgcca
      781 tctggatcca gagtcagaag ggtcggcccg atcgtcggcg tggcattgct gaaccgcatt
      841 ttattcacgt caaaccatcg cccaacgaat ttaactgctt ctggcggctc gacctcaaaa
      901 ttgactgcgg ttcgcttggg cgaagccccg aaatctttta ggggatcggc ggcgtcaagt
      961 gcatgtagtc cctgaattag gagcgagaaa atatgaccgt gttgtttatc gagggcgatg
     1021 gattggcgtt tgattgctgt gactattttc ccggtctgtg agaaatgagc acgcccgtca
     1081 gtgtgatgtg aatacttaac gaggtgtgac gtgacctttc cgccatgttc aaggggtact
     1141 tcggtttgtc gcgtgccggt agctggaatc gatgatgagg aaagaattcc tgtgcggtgc
     1201 ctgaaatagg ggaaagtaat aaataggctg ccatccgccg caaaaactat ttggaaggct
     1261 ttcgacatca cctcgccagc cttgaccacg cagaccacac gctcactgcg aaacgcatcg
     1321 atattaccgg cctggaatcc tgtcattcta aaagtgtagt acgggcgcgc attcgcgcgg
     1381 gcatatagaa gcgggattgc cgagtaattc aaggtttcgg atgggcgggt ggcccgctca
     1441 agcctgatgt tggcttgagt ggaaattgag aatcttcgag tcaaattcga ttagtacgag
     1501 agcatctggc gatttttatt tcccagacaa cgaaaaccgc gttgtgtggg ccacccgccc
     1561 aatttcattt ccgagaaact gggtcagtca gtctgtcacc acatttggaa tgggccatcc
     1621 gcccatttgt cgttttcgat aggctcttct agtattgcga tttctcaagc gcctatggaa
     1681 acatcacaat ccaatctcgt cgcaacgcta cgcaggcttc ctctgttcac ggacttgagg
     1741 gagagtgaac tggttctgat tgccgaacga atcaccgtga agcagcttag ccgaggtgag
     1801 attgtttttt ccgaaggcga tccctgccgg gaattgctaa tcgtaagaga cggcagggtg
     1861 aatcttttga aaactaccgt caatggaaga cggcaactca tcagcgttga gcgcgcagga
     1921 agttctcttt ctgaaacctc agcgtttgac ggccatgcct atccggtcac cgccgagact
     1981 gtggaggata cgactctatt gcgactggaa gcggaccatt ttcgcaaggt ttgcctgcaa
     2041 cacccggaga tggcgctgaa gatcatcaaa gtgctaggcc atcgcttgcg gcgcatgagc
     2101 cgccttattg aggacctctc attttcaact gttcgaggcc gcttggtggc gtatctagtt
     2161 catcttgccc gcgacgaagg ccgctcgatt tcccaaggga ttgagctcga gttgaccgaa
     2221 aacaacgaac agcttgcggc tcgactggga acggttcgcg aattggtttc tcgtaatcta
     2281 ggacgcttgc acaatgaagg tttgatccag atgtctcgac ggagagttcg aatccccaac
     2341 ctcgatgtat tggcggaaga aatagccaac atccgctaat ttgttttcat tttccgtgac
     2401 aaaagtcatc gcgggaacgc caacgttttc ctaggctgat tcaaagcgat ggaaacactc
     2461 gtcgctttgg aggactcatt ggcctacgtt attgctgaac catgcatcgg tgcgaaagac
     2521 acagcatgca tcgacgtctg cccggtagac gccatacatc cgggaacgaa tgaagcgaat
     2581 gctgctaccg tggcgcaact ctacatcaac ccagacgact gcatttgctg cgggatgtgt
     2641 gtaccagttt gtccggtagg agctatctac gcggaggaag atctagccga gaagtggaag
     2701 cactacatcc agatcaatcg cgattggttc ggcagttccg aacacatcta atttccagcg
     2761 agaccataac aggtttttca gaatacccac tcaagccaaa acccggcttg agtgggccac
     2821 ccgaccggcc gcttaccgta ttgccgcaac aggatcgacg cgagtcgccc cctcgcaggc
     2881 atgtaacaca ccagtagcga catcgccgcc aaaaaccagg agcgcgagcg aagccgcgac
     2941 ctgcgccacc accagagcgc tgagcaaccg cgtttgtgcg agcctcccac ccgccttaca
     3001 attcgcgaat cgcggtcacg gggttccggc gcatcgctcg acgtgccgga aagtagcatg
     3061 ccagcaaaga cattgctccc agaagcatga cgacggaagc catggtgaat ggatcgttgg
     3121 ctccgattcc atacaacagc cgcgcgatga gacgtgtcag ccccaaggca aacgccaatc
     3181 ccagcgccag ccccacgcct gtgagccacg ctccctgcaa cagcacgagt ctcagcacat
     3241 caacgcggga agcgcccaga gccattcgca ctccgatctc gtgggtccgc atttgggttc
     3301 gatatgtcac gactccgtag attccggtta ctgcaagaac cagcccgatc agcgcgaaca
     3361 ttcctgccag cgtgctctcg attacggcga acgtgctcgc cagatgggta ctctcgcgca
     3421 tggaacgcac atcgaaaacc ggcagccgtg gatcgatttc gtggatagcg ttttccaccg
     3481 ccggctccag gtcaaccgga ttcccctggg ttttcacctg caccatggtc tcgtaccccg
     3541 ggtgctggaa aaaactcata tagatcatag gctccggtga ctcattcacg aaagtatgtg
     3601 ttgaattcct cgcaacgccg acaaccgtgt gaaggctgcc ccatatcctg agtctctttc
     3661 ccagcggatc ctctcccggc cagtagtgtc tcgccgcagt ctgatctacg atgatgacac
     3721 tcggggcctt ttcatcgtca ttcagagtaa actcgcgtcc ttccagaatg ggtatatgaa
     3781 gcgattcgaa ataccgcggg cctacctcgg cattctccac cagcaaagat tcatgggggt
     3841 gcggcacata cccttccgga tacgcatcgt tcctcttttg gatcagagtc atgggaatcc
     3901 agtccgtaag agacgcaacc ttcacgccgg gtaatgccga cacgcgatca agtatcttgt
     3961 gacggatcaa ctgtgcctcg tcgttgctgt agccggcgat attcagcccc acggacgccg
     4021 tgagaatgtg gtcctgctcg aatccgggat ttgcgtcggc gaggttccgc agtgttcgca
     4081 gaagtagccc ggagcacaac agcagcggaa gtgaaagcgc gatttgcgtc accaccagcg
     4141 cgctgagcaa tttccggttg cgtgatccgc cggagatact tgcagactcg gccttgagta
     4201 cctcaacggc cggcgcgtag gacgaacgcc aggcggggag cgcgcagcac agcatgccag
     4261 ccaacaggga gaacaccgca atcccgatta ccacgttgta atccatgctc ccgttcagaa
     4321 tgattggagc cgagctcgcc cgaaagaacc atgcgaatgt cttagacgtc cacgatgtca
     4381 acaaaagggc cgcggctcct gcgacgatgg atagggccac gccttccagc accatctgcc
     4441 gcaccagttg catgcgattg gcccctagcg actgccggat cgcaagctcg cgccgccggg
     4501 atacaaagcg caccagcgtg agcgtagcca cgttcgcgca ggtcagcaac agcaccaggc
     4561 cggcgaaggc cagcagaatg ggcagcgtcg ccgccatgta tccgttggcg ccgaatggcg
     4621 aacgccacat aggatcgagc gtaatccggt tatcgccgag atgctggtcc ggatacgcgg
     4681 ccacaatatg gttcatctgt gtgtcgagat cctgggctgc ctggccccgg ctcacgcccg
     4741 gccgcaatct tcctattagg attagccaga ccgaatcgcg atgggtcatc cgccacggat
     4801 cggtcccgag gggatcgagc gttacccaca gatcgtcacg cagtccgggc gctgctccgg
     4861 taaaaccttc cggcgccaca ccgatcaccg tcacaggatg ccgggcgacc tcgatcgact
     4921 tgcccacaat tgccgggtct ttggaataac gtgtcttcca gagtgagtaa cccagcacca
     4981 cgttgggaat tgcacgggac tcctcttccg gcagaaagaa acgccccagc accggcttga
     5041 tgccgagcac atcgaaatag ttcgccgata cgttcgcaat atagactcgc tccggctgcg
     5101 cgccgccagt cagcgcgatc caatcgttat gatatgcaag tattccggcg agggtgtggt
     5161 tctgctcgcg cagatcgcga tagtcgagat acgagaacgg cggagtcgga gagaagttac
     5221 gttccccccg ctgcaggctt acgagttcgc ccgcatcccg tgcgccagga atcggacgca
     5281 acatggtgcc gtccatccag ctgaacacgg tgctgttggc gcaaatagca acggccagca
     5341 tcgcggtcgc ggtgatcgca aaaccggggc tcttgcgcaa ctgccgcaag gcaaagcgaa
     5401 tatcctgaag cagtcctgcc attacaatct cctcgacgac cgctgctctt tgctcttggg
     5461 aatgagagct aggtttaatt catcgcacgc actgtgccaa agcccgttta aggactaaga
     5521 gattgcccat cagcaatata gcgtgtactg gagcttccat tccggctcgc ctgctgtcca
     5581 tttgtgggcc tgcgtgtccg ttttcgaata gctaccgcta gcgcgggtgg ccctcgataa
     5641 gaacgaaggt ggtgtcaagc acaaacgtat cccgtcggtg ttttgacacc gtgttttttg
     5701 ccgccagaga ggttgaggac caagactctg gaaatctcac gctgaatcac ctgcggtgga
     5761 ga
//
LOCUS       DATNDT010000238         9708 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1729904, whole genome shotgun sequence.
ACCESSION   DATNDT010000238 DATNDT010000000
VERSION     DATNDT010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9708
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1729904"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1340
                     /locus_tag="VN788_07600"
     CDS             <1..1340
                     /locus_tag="VN788_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXU20430.1"
                     /translation="TVLRRNTEKQWCPVNSGQTIISATQEKAKEPAGERSSTRMARRR
                     FQKGYLFKKGKVWVARWWEDIIRVDGTPGRARRFVTLGDTPKHVAQKALERKLQPINY
                     GRQRPQSVMTFDQFVRERWIPAEMPLLDPASLKLDPKGLTNPGLALHNRPGSVETYGS
                     YLRCHLIARFGEKRLSDLTRWDVQTFLSEKLKQGYAGAHVHGMRTTLSKVLQAAVEWD
                     FLENNPARGCRIAHQEPMKQRTYLTAEHVQRLTRGLPDPCRTIVLVAVFTGLRIGEIL
                     ALRWGRIDFLGQTLSVEETYSDRFGPPKTRSSKRVIPMSTSLVRLLEVHRRSCCGMEP
                     ENLVFTTPKGTPLSPKNLRNRVLEPTRKSLGLPRLSWHTFRHTHTTWLGEVGVSPRIA
                     QSILGHSDVAVTLNIYTQVVPESQRLAMEKVAAVLDTNGHQNGPKIGPTVRPQ"
     gene            complement(1367..1441)
                     /locus_tag="VN788_07605"
     tRNA            complement(1367..1441)
                     /locus_tag="VN788_07605"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1407..1409),aa:Lys,seq:ctt)
     gene            complement(1521..2474)
                     /locus_tag="VN788_07610"
     CDS             complement(1521..2474)
                     /locus_tag="VN788_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoesterase"
                     /protein_id="HXU20431.1"
                     /translation="MKIRVCFHDKCFDGASSAAIFSRFYRERIEPHVQFFYTGMTHKA
                     SQPFEDGIFDGDENAVVDFKYSSSPKLTWWFDHHQSAFLKQEDAEHFRQDRSGKKFYD
                     PTYKSCTKFLATVASEKFGFDARPLADLVHWGDIIDGAQFASPEEAVELAQPAMQLAL
                     VIEAAPEANLVPQLIPYLSELPITEIVELPLIKKHLGPLLERHRRSIDILRERVDARN
                     GVIYFDVSDLDLEGYNKFVPYYLFPDALYSVGVSASPVRAKISVGTNPWKEASAEVNL
                     AALCEKYGGGGHARVAAISLGPGDLVRARQVAKEITSSLRH"
     gene            complement(2624..4642)
                     /locus_tag="VN788_07615"
     CDS             complement(2624..4642)
                     /locus_tag="VN788_07615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXU20432.1"
                     /translation="MARFSPRRLSVWLRRTSIFDRVAIGVLILDAIEGIVPHLGVRRL
                     PFSSFVNFLGFAALVYLAARVILWVRVRALWGLRNRLIVAYVFMAVIPVVLLLTMVAV
                     SFILLELQIGAHLLRDDLGRHLTVIAADRNAIAAALRHEPGLKVDEPVSPTSSATTDP
                     VLLRPGVASVIGAAQAEWPGLRVYLNHGQQLVRTPKTCPDSCLAEFRGQLWFASAERL
                     PVPGGHATVLVIAPITPALLDSLPSKLGPIELTLLDPTSQPPNGSRTVEFGKSRYVLG
                     EQISSRHRAVTPPTNWFDMSVDGVATLEAVRADLGQDTQDRPVLAAFSLRLSAVNKDL
                     LTSMGAFGPYLVDILIIAAIIFLALEIAAFATGTVLTRTITRWVADLYDATLHVRRGD
                     FSHRVRVTKRDQLGALGESFNDMTSSISELIEGERERQRLENEVAIAHEVQQQLFPRV
                     LPAVAGLELAAICRPARVVSGDYYDFIQLGPARVAIALADISGKGIFAALLMASLQAA
                     LRSIASLGDQCNTADLVSRLNGHLFRNTSDDRYATLFYAIYDGDAKTLTYTNAGHPAP
                     FFLCDGSVQSLDEGGTVVGLFENPTYQQRTIRISHGSLLVVFSDGLVEPESVYGEEFG
                     VERLKAEMLRQRGLSPPQLAESLIDAAERWSGTAEQADDMTVVIARMP"
     gene            complement(4662..6212)
                     /locus_tag="VN788_07620"
     CDS             complement(4662..6212)
                     /locus_tag="VN788_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXU20433.1"
                     /translation="MASQLLATKPLDVIMEEAKETGESSLRRALGPLNLVTLGIGAII
                     GAGIFVLTGQAAATSAGPAIVVSFILSGIICAFAGLCYAEFASLIPIAGSAYTYGYAT
                     LGEIFAWIIGWDLILEYAFGAATVASGWSGYLVSLLDDLGLHIGPRWIATPGTELVLY
                     KGHWAAIGSLQQALASAGVNPATLPHAVAVFNLVAFFGIAIATFVLVVGIRESANFNS
                     GIVILKVAIVLVFIAVAGSFVLRHGSLAAANWHPFIPPNTGKFGEYGLSGVARGAAVI
                     FFAYIGFDAVSTAAQEAKNPQRDMPIGIIGSLVICTILYILVSGLLTAVVPYTDLNVA
                     DPVAIAIDATGVRWGSLLVKAGAVAGLGSVMLVMLLGQSRVFYSMSRDGLLPKWAGAI
                     HSRFRTPWISSIVVGVCVACFASLIPIGDLAVLVNIGTLLAFIIVCAGVWILRRKRPD
                     LPRPFKTPLVPLVPILGILTSGAAMLSLPAKTWWRLLIWLVIGLFIYAFYGRTHSKVH
                     KASLNRIP"
     gene            complement(6276..7814)
                     /locus_tag="VN788_07625"
     CDS             complement(6276..7814)
                     /locus_tag="VN788_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXU20434.1"
                     /translation="MADQSQAIEFATARPSAELVKGLGLFDSTMLVIGSMIGSGIFIV
                     SADIAHQVQSPALLILVWVFSGLMTLIGALSYGELAAAMPHAGGQYVYLREAFGPLAG
                     FLYGWTLLLVIQTATIAAVAIAFSKFTAVLAPWFSASHWIWKIGTFGPWHLWFGSLGP
                     YNVGLNRQNVLAIASIVVLTWINTRGLRLGKIVQNIFTVTKIASLALLVLLGLWLSTA
                     IARTANFTDFWRNAGLSLRHPYPPDAPSWMIGTITLIAVAMVGSLFSMDAWNNITFTA
                     AEVKNPGRNLPISLALGTGTVILLYTLANFAYLRVLPLSGNPNGATVLSRGIEFAADG
                     RVATAAVQAMLGPAGAILMALAILISTFGCNNGLILSGARVYYAMAKDRLFFRSAGTV
                     NRHHAPGIALVVQCLWASVLCLSGTYGQLLDFLIFAVLIFYILTLMGLFLLRWNRPKL
                     ERPYRAFGYPFLPALYIVMAVFLEIQLLRYKPQYTWPGLIIVLLGLPVYFIWNWAGKR
                     ALKT"
     gene            complement(7823..8470)
                     /gene="thiE"
                     /locus_tag="VN788_07630"
     CDS             complement(7823..8470)
                     /gene="thiE"
                     /locus_tag="VN788_07630"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXU20435.1"
                     /translation="MFPPLYAILDPDFTTVPLSQLGASLATAGVQLMQLRCKHASAAR
                     MHALAAELATLLRPMGARLIVNDRADVAALSGASGVHVGQEDLPVEDARRFCASPLWV
                     GVSTHNLEQLREADRTSADYIAVGPVFHTGTKANPDPVVGIDFIRKARQLTRKPLVAI
                     GGITLESAAEVFRAGADSLAVISDMIATAEPAQRAAGYLAIAGRERANRDQGHSE"
     gene            complement(8502..9497)
                     /locus_tag="VN788_07635"
     CDS             complement(8502..9497)
                     /locus_tag="VN788_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015186398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXU20436.1"
                     /translation="MQKRKLGKSNLEVSAIGLGCMGMSFGFGPPADKKEMISLIRTAV
                     ERGITFFDTAEVYGPFANEELVGEALAPLRKQVVIATKFGFKIDPAAERPSIGTDSRP
                     DHIKKVAEASLKRLKTDVIDLFYQHRVDLDVPIEEVAGAVKELIQQGKVKHFGLSEAG
                     VQTIRRAHAVQPVAALQSEYSLWWREPEAEVIPTLEELGIGFVPFSPLGKGFLTGKID
                     ENTKFDKSDFRNMVPRFTPENRKANQALVDLVGRFAQQKKATPAQIALAWLLEQKPWI
                     APIPGTTKLHRLEENIGATTVELSPEDLRELGVAASKIAVQGARYPEQMQKMVGR"
BASE COUNT         2271 a         2843 c         2720 g         1874 t
ORIGIN      
        1 agacagtgct gcgccgcaac acagaaaagc agtggtgtcc cgtgaatagc ggacagacta
       61 taatctccgc gacgcaggaa aaggcgaaag aaccagcggg cgaaaggagc agcacccgca
      121 tggctcgacg ccgtttccaa aaagggtatt tgttcaagaa aggcaaggtc tgggtagcac
      181 gctggtggga ggacattatt cgggtagacg ggacgccggg aagggctcgg cgcttcgtta
      241 cactcggcga caccccaaaa catgtagccc agaaggcact cgagcggaag ctgcaaccca
      301 tcaactacgg aaggcagcgc ccgcaatctg taatgacgtt tgatcagttc gttcgtgaac
      361 gctggatacc tgcggagatg ccgttgctcg atcccgcatc actgaagctg gatcctaagg
      421 gtcttaccaa tcccggactc gccctgcaca atcggccggg gtcggttgag acatacggtt
      481 cctaccttcg gtgccatttg attgcgcgct tcggcgagaa gcgcctcagc gacttgaccc
      541 gctgggacgt gcaaacattc ttgtccgaaa agctcaaaca aggctatgcc ggtgcccacg
      601 ttcacggaat gcgaaccacg ttgagcaagg tgttgcaggc agcagtcgaa tgggacttct
      661 tagaaaacaa tcccgctcgc ggctgtcgca tcgctcacca agaaccaatg aagcagcgca
      721 cgtatcttac cgcggagcat gtgcaacgcc ttacccgagg attgcctgat ccgtgccgta
      781 cgattgtgct cgtcgctgtg ttcacgggac tccggatcgg cgaaatcctc gcgctccgtt
      841 gggggcgtat tgattttctc ggtcaaacgc tctcggtcga ggaaacctat tcggaccggt
      901 ttgggccgcc gaaaacgcga agcagcaaac gtgttattcc gatgagcaca tctcttgtgc
      961 gattgctcga agttcatcgg agatcttgct gtgggatgga gccggaaaac cttgtgttca
     1021 ccacacctaa gggaacgccg ctcagtccca agaacctgcg taaccgcgtg ctcgaaccca
     1081 ccaggaaatc gctggggcta ccgcgtttgt cgtggcacac ctttcgccat acgcacacga
     1141 cgtggctcgg cgaggtaggt gtgtccccgc ggatcgcgca gtctattctc ggtcattccg
     1201 acgtggcggt gacgctcaac atctatacgc aagtggttcc ggagtcgcag cgactcgcga
     1261 tggagaaggt cgcggcggtt ttggacacta atggacacca aaacggcccg aaaatagggc
     1321 caactgtgag accacagtaa agtagacaaa gaacttggga gagaagtggt gagccgcgga
     1381 ggactcgaac ctccaacccg ctgattaaga gtcagctgct ctaccattga gctagcggcc
     1441 cggcgaattc cgattctaac atgatcaaac tgcttcccca tgcgtccctg gggtggcctc
     1501 gggtggcctg gaataggtcc ttaatgccgt agcgacgacg taatttcctt cgccacctgc
     1561 cgtgcgcgga caagatcgcc gggccccagc gagatggccg ccacgcgcgc gtgaccacct
     1621 ccgccgtatt tctcgcacag cgcggcaagg tttacttcgg ccgacgcttc cttccagggg
     1681 ttggtgccga cggatatttt ggcgcgaact ggcgacgcgc tcactcccac ggaatagagg
     1741 gcgtcgggaa agagataata gggcacgaac ttgttgtacc cctcgagatc caggtcgctc
     1801 acgtcgaaat agatgactcc gtttctcgcg tccacccgct cgcgcagaat atcgatggag
     1861 cggcggtgcc gctcaagcaa cggcccgagg tgcttcttga tcaacggcag ttctacaatt
     1921 tcagtaatcg gaagctcgga aaggtaggga atcaactgcg gaaccaggtt ggcctccggc
     1981 gcggcttcga tcaccagggc aagctgcatg gctggctgcg ctaattccac ggcctcttcg
     2041 ggagacgcga attgggcacc gtcgatgatg tcgccccaat gaaccaagtc agccaaggga
     2101 cgcgcatcga atccgaattt ttcggaagca acagtggcta aaaatttggt gcaggacttg
     2161 taagtaggat catagaattt tttgccggag cgatcctgtc ggaagtgctc ggcgtcctcc
     2221 tgctttaaaa aagcactttg gtgatgatcg aaccaccacg tcaatttcgg agagctcgaa
     2281 tatttgaagt cgacgactgc gttttcatct ccatcgaaga tgccgtcctc gaaaggctgg
     2341 gaagccttgt gagtcattcc cgtgtagaaa aattgcacat gcggctcaat tcgttcgcga
     2401 taaaagcggg aaaatatcgc ggcggaggaa gcgccgtcga aacacttgtc atgaaaacat
     2461 acacgaattt tcaacgattt ctctccgtcc accgtgaacg cggccgtaaa acctgcaagg
     2521 ggaagtcaat taagatggcg ggtccatctg cgcttgtcaa tagcgaacct ggaggtcggc
     2581 cttgtcgcca tcgccgccgc accaatgctg ggacgaaacc gtcctagggc atccgggcga
     2641 tcacaaccgt catatcgtcg gcttgttccg ctgtgccgga ccagcgctcg gcggcatcga
     2701 taagactttc agcgagttga ggcggcgaga gaccgcgctg gcggagcatt tccgctttca
     2761 atctctcgac gccgaattcc tctccgtaaa cactttccgg ttccacaagg ccatcgctga
     2821 acacgactag cagactaccg tgcgatatac ggattgtacg ttgctggtat gtgggattct
     2881 cgaagaggcc caccaccgtt ccgccctcat ccagcgattg gacgctgcca tcacatagaa
     2941 aaaatggcgc cggatgaccg gcgttagtgt aggtaagtgt tttcgcgtct ccatcgtata
     3001 tggcgtagaa aagtgtcgcg tagcgatcgt ctgaagtgtt tctgaaaagg tgcccgttga
     3061 gccgcgacac caggtcggcg gtgttgcact ggtcgccgag cgaggcgatg cttcgcaggg
     3121 ccgcctgaag gctggccatc aggagcgcgg cgaagattcc cttgccgctg atgtcagcca
     3181 gggcaatcgc aacgcgtgcc ggccccaatt gaatgaaatc atagtaatca ccgctaacca
     3241 cgcgcgcggg ccggcagatg gcagcaagct cgagtccagc aacggcgggc agaacgcgag
     3301 ggaaaagctg ctgttgcacc tcgtgagcga tggcgacttc gttttccagg cgctgccgct
     3361 cccgttctcc ttcgatgagc tcggaaatcg agctagtcat gtcattgaac gactccccga
     3421 gggcgccaag ctgatcccgt ttcgtgacgc gaacacggtg gctaaaatcg ccacgccgga
     3481 cgtgcagcgt cgcgtcatac aggtcagcca cccaacgagt gatcgtccgc gtgaggaccg
     3541 tgccagtggc gaatgcggcg atttccagcg ccaaaaatat gatcgcggcg atgatgagga
     3601 tatccacgag gtacggccca aaagctccca tggaggtcaa caggtccttg ttcaccgcag
     3661 acaaacgcag cgaaaacgca gccagcaccg gccgatcctg cgtgtcttgt cccagatcgg
     3721 cacggacagc ttccaaggta gcgacgccat ccactgacat atcgaaccaa tttgtgggtg
     3781 gcgtaacggc tcggtgacga ctggaaattt gctctcccag aacgtatcga gattttccaa
     3841 attcaaccgt gcgcgaacca ttggggggct ggctcgttgg atcaagcaga gtcagttcga
     3901 tcgggcccaa tttagaaggc aagctatcga gaagcgcggg cgtgataggc gcgattacga
     3961 gcacagtggc gtggccccct ggcaccggca gccgttcggc cgatgcgaac cagagctgcc
     4021 cgcgaaattc agcaaggcat gagtccggac aggtcttcgg agttcgcaca agctgctggc
     4081 cgtgattcaa atagacgcgc aagccgggcc actccgcttg tgcggctccg attacgctgg
     4141 cgacgcccgg acgcagcaac acgggatccg tggtcgcgga ggacgtcgga gaaaccggct
     4201 cgtcaacctt caaaccgggt tcatgcctca gcgcggcggc gatggcattc ctgtcggcgg
     4261 cgatcaccgt aagatgccgt ccgagatcgt cgcgcagcag gtgcgctccg atttgaagtt
     4321 cgagcaaaat aaatgaaacg gccaccatgg ttaggagcag caccaccggg atcacggcca
     4381 tgaacacgta cgcgacgatc aggcgattcc gcaagcccca tagagcgcgc acccgcaccc
     4441 agagaatcac acgcgcggcc agatagacga gcgccgcaaa accgagaaag ttcacgaaag
     4501 acgaaaaggg aagcctgcga acgcctaaat gaggaacaat cccctcaatg gcgtcgagga
     4561 tcagtacacc aatggccacg cggtcgaaga tcgaggttcg gcgcagccat accgataaac
     4621 gacgcggaga gaatcgggcc ataaaagaag cccgaattat gtcatggaat tctattcaga
     4681 gatgccttgt ggactttgct atgggttcgg ccataaaacg cgtaaatgaa cagtccgatg
     4741 accagccaga taagcaaacg ccaccaggtt ttcgccggca ggctcagcat tgccgccccg
     4801 gacgtgagga ttccgagaat cggcactagg ggaaccagcg gcgtcttgaa tggccgcgga
     4861 agatcgggcc gcttcctgcg cagaatccaa acgcccgcgc atacgataat gaacgccaga
     4921 agcgtcccaa tattcacgag taccgccaga tctccgatgg gaatcagtga agcgaagcac
     4981 gcgacgcaca cgcctacaac gatcgaagag atccacggcg tcctgaagcg cgaatgaatc
     5041 gctcccgccc atttcggtag cagcccatcg cgcgacatcg aatagaacac gcgggactga
     5101 ccaagcagca tcaccagcat aacgctgcca agaccggcga cagcgccggc tttcactagt
     5161 aagctgcccc agcgcacgcc cgtggcgtca atggcgatgg cgacagggtc ggcaacgttc
     5221 aggtctgtgt agggaaccac ggccgtcaac agaccggaca ccaatatgta gagaatagtg
     5281 cagatcacca gtgaaccgat aatcccgatg ggcatgtccc tttggggatt tttggcttcc
     5341 tgcgcggcag ttgaaaccgc gtcgaacccg atgtatgcaa aaaagatcac ggcggcgcct
     5401 cgggccactc ccgagaggcc gtattcgccg aatttgcccg tgtttggcgg aatgaacgga
     5461 tgccagttag cggctgccag cgagccatgt cgcaggacga aagagcccgc aacggcgata
     5521 aacaccaata cgatcgccac cttgaggatt acgattccag aattaaagtt ggccgattcc
     5581 cggatgccca ccaccagaac gaaggtcgcg atggctattc caaagaaggc aaccaaattg
     5641 aaaacggcga ccgcgtgagg aagcgttgcc gggttgactc ccgcgcttgc aagcgcttgc
     5701 tgcagcgagc cgatcgcagc ccagtgcccc ttgtaaagga ccagctcggt tcccggcgtg
     5761 gcaatccatc tcggcccaat gtggagtcct aagtcgtcga gaaggctgac gagataccca
     5821 ctccatccgg aggccaccgt cgcggcgcca aaggcatact cgaggatcaa atcccaaccg
     5881 atgatccacg cgaaaatttc gcccagcgtc gcatagccat acgtgtacgc cgaaccagcg
     5941 atgggaatca gcgaggcgaa ctctgcgtag cagaggcccg caaatgcgca gatgataccc
     6001 gacaaaatga atgagacaac gatcgcaggt cccgcgctgg tggctgcggc ttgccctgtc
     6061 aatacgaaga tgccggcccc gatgatggcg ccaatgccca gagtgaccag attgaggggg
     6121 ccaagtgcgc gacgcaacga ggactcgcct gtttccttgg cctcctccat tattacgtcg
     6181 agcggttttg tggcgagcag ctggcttgcc atgctttgtt tccccgtttc agagtcgagc
     6241 gtcggcatat tcgtcatgcc tctcgcaaaa attggctacg ttttaagcgc ccgcttcccc
     6301 gcccagttcc aaatgaagta aaccggcaaa cccaacaaaa cgatgattag tcccggccaa
     6361 gtgtactgtg gtttatacct caacaattga atttcaagaa acacagccat cacgatgtac
     6421 agcgctggca aaaaggggta tccaaaagcg cgatagggac gctctaattt cggacgattc
     6481 cagcgcagca ggaatagtcc catcaaggtg agaatgtaga agatcagcac cgcgaaaatc
     6541 agaaaatcga gcaattgccc gtacgtcccc gaaaggcaga gcacagacgc ccataggcat
     6601 tggaccacga gcgcgattcc aggcgcgtga tggcggttca ccgtccccgc ggacctgaaa
     6661 aaaagcctgt ccttcgccat cgcatagtac actcgggcac ccgaaagaat taggccgtta
     6721 ttgcatccaa aagtcgaaat caaaattgcg agcgccatca agatagcccc cgccggtccc
     6781 agcatcgctt ggaccgcggc agtcgcaacg cgcccgtctg ctgcaaattc gatccccctg
     6841 gaaagcacgg ttgcgccgtt cggattcccg gaaagcggca gtactcgtag atatgcaaaa
     6901 ttcgccaacg tataaagcaa aatcacggtg cctgtgccca gcgccagaga aatcggcaga
     6961 ttgcggccgg gattctttac ttcggcggca gtgaacgtga tgttgttcca cgcgtccatc
     7021 gagaaaagtg atccgaccat cgccacggca atcagcgtga tggtcccaat catccacgac
     7081 ggcgcatcgg gtgggtaagg atgtctgagc gacaagccgg cgttgcgcca aaaatccgtg
     7141 aagtttgccg tgcgcgcgat cgcagtgctg agccaaagtc ccagcagaac caacagcgcg
     7201 agcgacgcga ttttggtcac tgtaaaaatg ttttggacaa tcttgcctag gcgcagcccg
     7261 cgcgtattga tccacgtgag cacgacaatc gaagcgatcg ccagcacgtt ctgccgattc
     7321 agccccacgt tgtacgggcc gagcgaaccg aaccagagat gccaggggcc aaacgtccca
     7381 atcttccata tccagtgaga agctgaaaac cagggcgcca gcacggccgt aaattttgag
     7441 aatgcaatgg cgacggcggc gatcgtcgct gtctgaatca ccagcagcaa ggtccatccg
     7501 tacagaaatc ctgccaatgg cccaaacgcc tcgcgcagat acacgtattg cccgccggca
     7561 tgcggcattg cggccgcaag ttcgccgtag ctcaaggccc ctatcagcgt catgagcccc
     7621 gaaaaaaccc aaacaagaat cagcaaggct ggactttgta cctggtgagc aatatccgcc
     7681 gaaacgatga aaattcccga gccgatcatc gacccgatca caagcatcgt cgaatcgaaa
     7741 aggccgagcc cttttacgag ctcggccgag ggccgagcgg ttgcgaattc gattgcctgc
     7801 gattgatctg ccattccgcc cgttattcgg aatgcccttg gtcccggttg gcgcgttccc
     7861 tacccgcaat tgccaagtat ccagcggcgc gttgcgctgg ctcggctgtg gcgatcatgt
     7921 cgcttataac cgccagcgaa tccgcaccgg cccggaacac ttccgcagct gattccagcg
     7981 taatcccgcc aatcgccacc agcggcttgc gagtcaactg gcgggccttt cgtataaaat
     8041 caatgccgac caccggatca ggatttgcct tggtgcctgt gtggaatacc ggccccactg
     8101 caatatagtc tgccgaagtt ctgtccgctt cgcgcaattg ctcaaggttg tgtgtcgaaa
     8161 cgcccaccca cagcggtgaa gcgcaaaaac gtcgcgcgtc ctccacggga agatcctcct
     8221 gccccacgtg aacgccggac gccccggaaa gcgcggcaac atcggcgcgg tcattcacga
     8281 taagccgcgc gcccatcggc cgcagtagcg tagctaattc ggcggcaagc gcatgcattc
     8341 gggcggccga ggcatgcttg cagcgaagct gcatcaattg aacgcccgcc gtcgcgagcg
     8401 atgcgccgag ctgcgatagc ggcaccgttg tgaaatcagg atcgagaatc gcatacagcg
     8461 gcggaaacaa tgcgcctcac acagtggcag cttcgggtaa tttaacggcc caccattttt
     8521 tgcatctgtt cgggatagcg ggcaccctgc accgcgatct ttgaggctgc gacgcccagt
     8581 tcccggagat cttcgggcga aagctccacc gtagtggctc cgatgttttc ttccagccga
     8641 tgcagcttcg tcgtacccgg aatcggtgca atccacggtt tctgctcaag cagccaagcg
     8701 agcgcgatct gcgcgggcgt cgctttcttc tgttgcgcaa atctgccaac cagatcaacc
     8761 aatgcctgat tcgcttttcg attctctggc gtaaagcgag gcaccatgtt ccggaaatcg
     8821 cttttgtcga acttcgtgtt ctcgtcgatc tttccggtca gaaagccttt tcccaaaggg
     8881 ctaaatggaa cgaagccgat cccgagttct tcgagcgtcg ggatcacctc cgcttcaggt
     8941 tccctccacc aaagggaata ttcgctctgc agtgcggcaa ccggctgcac tgcatgcgcg
     9001 cgacggatgg tctgaactcc tgcttcggaa agtccgaagt gtttcacctt gccttgttga
     9061 atcaattcct tcacagctcc cgccacttct tcaattggca catcgaggtc aacacgatgc
     9121 tggtagaaca ggtctatgac gtcggtcttg agccgcttta gcgaggcctc ggcgactttc
     9181 ttgatgtggt ctggccggct gtccgtccca atcgaaggtc gttcggctgc aggatcgatt
     9241 ttgaacccga acttcgtggc gatcaccact tgcttacgta atggcgcgag cgcctcgccc
     9301 acgagctcct cgttcgcgaa cgggccataa acctcggcgg tgtcgaagaa cgtgatgccg
     9361 cgttcaacgg ccgtccgaat aagggagatc atctccttct tgtccgcagg cgggccgaaa
     9421 ccaaagctca ttcccatgca gccaagcccg atagccgaaa cttccaaatt actctttcca
     9481 agtttgcgct tttgcactgt ttcctccgtc ggtgtgtagc gccacccctt cagggtggca
     9541 tcttgactag tctgcttggc ctgactcggc catctttcgc cagccgcttt cagggctagg
     9601 aactagtcgg ttggcgccca taccacaact caacaaagcc attcttgtaa gcgacgacgt
     9661 ccctcagacg ccacctatgt tcgaaaccgg aattaccgaa taagcgca
//
LOCUS       DATNDT010000239         4857 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1166487, whole genome shotgun sequence.
ACCESSION   DATNDT010000239 DATNDT010000000
VERSION     DATNDT010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4857)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4857)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4857
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166487"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1873
                     /locus_tag="VN788_07640"
     CDS             <1..1873
                     /locus_tag="VN788_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HXU20437.1"
                     /translation="RTWDVRLQLERMSSSVVVSDTAEPTTAETAPDLVDVITKEQIDQ
                     RQAIWMIDMLASEQGVSFDRGGPFGGVTGLFLDGGNSNYTKVLVDGTPVNQPGGAIDF
                     SNFTLLNVDKIEIVHGASSALYGSDAMDGVVQIFTHRGTTTVPQITLEGDGGTFGTGN
                     GSAQLSGLLGSRFDYSAGGQYFSTEGQGPGDYFRDATASGNFGWKFSDTDSLRLSLRN
                     SSSDAGQPGQTLLPGQTVFGQHNDLHDFSANLSWSFTTGEHWQHRVSGLESRFQDNDF
                     SPFFGSLISKYNRAGFEGQSTYLFHGGEITVGYENEVEVGPTETRHNQAGYLEVRRQF
                     GRRLTAVIGGRAEANGFFGTRFVPRVGASYALRDGQGFWGATRLRVSYGQGIKEPEIL
                     PSDCSPQLAPEESRTVNAGIDQLFASDRIHFSATYFHNDFHNIVSFAFLIPNPNCPAF
                     GGSFFNTESARADGANTSFEVKVTRWLSIAGNYTYDDSKVLVAGPEFDPSFEAGNRLF
                     RRPLHSANLIANAHFLRMNWNLAGYYVGRRSDSDFISTFVNGICQPGPGSPCITSNPS
                     YVRWDFANSIYLGHGLSTVAHIENLFDKHYQDEIGYPALGYNYRLGLTYTWGGEHPM"
     gene            1952..3442
                     /gene="gatB"
                     /locus_tag="VN788_07645"
     CDS             1952..3442
                     /gene="gatB"
                     /locus_tag="VN788_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HXU20438.1"
                     /translation="MSRTAQETPRIVPEVMARYEAVIGLEVHAHLQTMTKLFCGCSTR
                     FGDAPNSNTCPVCLGLPGALPVLNRKALDLALRASMALDCKIQECSRFARKNYFYPDL
                     PKGYQISMYELPLAIAGAIEIEVDGDKKRIGVTRLHMEDDAGKSLHEGFPDSDRWSYI
                     DFNRCGVPLIEIVSEPDLRSPAEAYQYLNTLKQILEYTEVSNCNMEEGSLRCDANVSV
                     RLRGTPKFGTKAEVKNLNSFRYLAHALEYEIERQIAIIESGSFITQETRLWNVAAGRT
                     EPMRSKEFAHDYRYFPDPDLLPVSVSAAMIEEVRRSMPELPAAKLARFQRDYGITEYD
                     AGVLTASQDLADYFESAVKAGAPAKTAASWISVELLRRLNDARKHISDCPVSPESLAE
                     LLRRVDKGDITAGSGKKVFALMFETGRQPAEIIAAEGLAQLTDTAAIEKIARALVAKS
                     PDNVAKYRAGNEGVFKFFVGQVMRATRGQANPQAVNEILKRILSGE"
     gene            3470..3931
                     /locus_tag="VN788_07650"
     CDS             3470..3931
                     /locus_tag="VN788_07650"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003391624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HXU20439.1"
                     /translation="MLKKDDPAPDFHATADDGKTVSLADYRGRNLILYFYPKADTTGC
                     THEAVEFRSALEQFTLANTAIVGCSGDSVQDQAKFKAKYNLNFPLLADTNFNVIEAYG
                     ARRMKTFFGKSPLGIVRMSFWIGPDGIIRKVWEKVTAKGHAAEVLAAIRTS"
     gene            complement(3955..>4857)
                     /locus_tag="VN788_07655"
     CDS             complement(3955..>4857)
                     /locus_tag="VN788_07655"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HXU20440.1"
                     /translation="IGAASMGSNGEALAEIAAMKRAGIVAVSDDGKPIASARLMRQVM
                     DYCNSLDLPVIDHCEDASLFAGGVMREGPQSIRLGLKGIPAQSESICTGRDVEVAALT
                     GARLHIAHMSTGESLEYVRSAKKRGLRVTCEVTPHHFTLTDEDVRYDSRYKMNPPLAS
                     REDRDALIEGLADGSVDAIATDHAPHHPASKDVEFDRAPFGITGFETALALGIAGLVD
                     GGKLSLMRLVELFTTGPARVLGMARKIASGEPADLTIFSTDREWTFRAAESPSKSRNT
                     PFDGRGFRGGPAATIVAGRIVFRS"
BASE COUNT         1071 a         1406 c         1374 g         1006 t
ORIGIN      
        1 gcgcacctgg gatgtgcgat tgcagcttga aagaatgtcc tcaagtgtgg tggtgagcga
       61 cacggcggag ccgacaaccg ccgaaaccgc gccggacctt gtggacgtga tcaccaaaga
      121 gcaaatcgat cagcggcaag cgatatggat gatcgacatg ctcgcctcgg aacaaggtgt
      181 gagcttcgat cgcgggggcc cgttcggcgg cgtcaccgga cttttcctgg acggcggaaa
      241 ttcgaattac acgaaggtgc ttgtggacgg cacgccggta aatcagccgg gcggcgccat
      301 cgatttctcg aatttcacgc ttctgaacgt ggacaaaatc gagatcgtcc acggcgcgtc
      361 cagcgctctt tacggctccg atgccatgga tggcgtggtg caaattttca cgcatcgcgg
      421 gacaacgaca gtgccgcaga tcaccctcga aggcgacggc ggcaccttcg gcacgggaaa
      481 cggtagcgcg caactcagcg gtttactggg aagccgattc gattattcgg cgggcggcca
      541 atacttttcc actgaagggc aggggcccgg cgattacttt cgcgacgcga ccgcctcggg
      601 aaattttggg tggaagtttt ccgatactga ttcattgcgt ctgtcgcttc gcaacagttc
      661 cagcgacgcc ggtcagccgg gacagacatt acttccaggg cagactgtct tcggccagca
      721 caacgatttg cacgattttt ccgcgaattt gagctggagt ttcacaaccg gagagcattg
      781 gcagcatcgg gtatccggtc tagaatcgcg ctttcaggat aatgatttct cgccgttctt
      841 tggctcgtta atcagcaagt acaatcgtgc gggcttcgag ggtcaatcaa cctacctttt
      901 ccacggggga gaaatcaccg tcggatacga gaacgaagtt gaagttggtc caacggagac
      961 gcgacacaac caagccgggt acctggaagt gcggcggcaa ttcggccggc ggctcaccgc
     1021 cgtgatcgga ggacgcgccg aagcgaacgg attctttggg acgcgatttg tgccgcgcgt
     1081 gggagcatcg tacgcgttgc gcgacggtca aggattctgg ggtgccacgc gcctgcgtgt
     1141 ctcttacggg cagggcatca aggagcctga aatcttgccg tcggattgca gcccccagct
     1201 agcgccggaa gagagccgca ccgtgaacgc cggcatcgat caactgtttg cgtctgaccg
     1261 gattcatttt tcggccacat actttcacaa cgactttcac aatatcgtga gctttgcttt
     1321 tttgatcccg aatcccaatt gcccagcttt cggtggaagt tttttcaata ctgaaagcgc
     1381 ccgcgcggat ggcgcgaaca cgtcgttcga ggtgaaggtt acacggtggc tgagcatcgc
     1441 tgggaattac acatacgacg actccaaggt gctcgtggca ggaccggagt tcgatccttc
     1501 gtttgaggct ggcaatcgtc tttttcggcg gccactgcac tcggcaaatc tgatcgcgaa
     1561 tgctcacttc ttacgaatga attggaatct cgcggggtat tacgttgggc gaaggtccga
     1621 tagcgatttc atcagcacgt tcgtgaatgg catttgccag ccagggccgg gaagcccgtg
     1681 cattaccagc aatccgagtt acgtgcgatg ggattttgcc aatagcattt atcttgggca
     1741 cggcctttcc actgtcgcgc acatcgagaa tcttttcgat aaacactacc aggatgaaat
     1801 tggctatccg gcgctgggtt acaactatcg cctcggcttg acgtatacgt ggggcggcga
     1861 gcacccaatg taatcgtcgc aagcgtgccg aagtttacgc gatcaccaaa ggtgtgtcac
     1921 accggcccga tgttataatc gtgcgcttgt catgagccga accgcacaag aaacgccgcg
     1981 aatcgtgccg gaagtgatgg cacgctacga agccgtcatt ggtctggagg tgcacgcgca
     2041 tctccaaacc atgaccaagc tgttttgcgg ctgctcgacg cgttttggcg acgcgcccaa
     2101 ttcgaataca tgcccggtgt gcctcggatt gccgggcgcg ctgcccgtgc ttaaccggaa
     2161 agcgctcgac ttggccctgc gtgcttcgat ggcgctggat tgcaaaattc aggaatgctc
     2221 ccggttcgct cgcaaaaact atttctaccc ggacctaccc aagggctacc aaatttccat
     2281 gtacgaactg ccgctcgcga ttgcgggagc gatcgagatt gaagtggacg gcgacaagaa
     2341 gcgcatcggc gtcacgcgcc tccacatgga ggatgacgca ggcaaaagtt tgcacgaagg
     2401 ctttcctgac tccgaccgct ggagctacat cgatttcaat cgctgcggtg tgccgctgat
     2461 cgaaatcgtt tccgagcctg atctgcgctc gccggccgag gcgtaccaat atctcaatac
     2521 gctcaaacag attctcgagt acacagaggt gagcaactgc aatatggagg aaggctcgtt
     2581 gcgctgcgat gcgaatgtca gcgtgcgcct gcgcggcact ccgaaattcg gcacgaaagc
     2641 cgaagtgaaa aatctcaatt ccttccgcta tctggctcat gcgctcgaat atgaaatcga
     2701 acgccagatc gcgatcatcg aatccggtag ctttatcacg caggaaacgc ggctctggaa
     2761 cgtggcggcc gggcgcaccg agccgatgcg ctcgaaggaa tttgcgcacg attaccgcta
     2821 ttttcccgat ccagatttgt tgcccgtctc tgtctcggcg gcgatgatcg aagaagtgcg
     2881 gcgctcgatg cccgaattgc ctgctgccaa gctggcgcgt tttcagagag actacggaat
     2941 cacggaatat gacgcgggcg ttctcacggc ttcgcaggat ctggcggatt atttcgaatc
     3001 ggccgtgaaa gcgggcgctc cggcaaaaac cgcggcaagc tggatctctg tcgagcttct
     3061 ccgccgccta aacgatgcgc gtaagcatat ttcggattgc cccgtatcgc ccgagtcgct
     3121 cgcggagttg ctgcgaagag tcgataaggg cgacatcacc gcgggaagtg ggaaaaaagt
     3181 attcgcgcta atgttcgaaa caggacgcca gcccgcggaa attattgccg ccgagggcct
     3241 cgcgcagctg acggataccg ctgccatcga gaaaatcgcc cgggctcttg tcgcaaaaag
     3301 tccggacaat gtggcgaaat atcgtgccgg caacgaaggc gtcttcaagt tttttgtggg
     3361 tcaggtaatg cgcgcaacgc gcggacaagc caatccccaa gcggtgaacg aaattctcaa
     3421 gcggattctc agcggcgagt agccagcgct gcaaaaacgg agatccaacg tgctgaaaaa
     3481 ggacgatccg gctcccgatt ttcacgccac ggccgacgac ggcaagaccg tttcgctcgc
     3541 cgactatcgc gggcgcaatc taattttgta tttttatccc aaagccgata ccactggctg
     3601 cacgcacgaa gccgtggaat ttcgcagcgc tctggaacaa ttcacgctcg ccaacaccgc
     3661 gattgtgggc tgcagcggcg attccgttca ggaccaagcg aaattcaaag ccaagtacaa
     3721 cctgaatttt ccgctcctcg ccgataccaa tttcaacgta attgaggcct acggcgctcg
     3781 ccggatgaaa acctttttcg ggaaatcacc gctcggaatc gtgcgcatgt cattctggat
     3841 tggaccggac ggaataatcc gtaaggtttg ggagaaagtg accgccaagg gccacgccgc
     3901 tgaagttctc gccgcaatcc gtaccagtta aaacgtcaca gcgcaaaatc taaatcaact
     3961 tcgaaaaaca attcgcccgg caacaatagt tgccgccggt ccgccacgaa aaccgcgtcc
     4021 atcgaaaggc gtgttgcgcg atttgcttgg cgattccgcc gcgcgaaaag tccactcgcg
     4081 gtcggtggag aaaattgtaa ggtcggcggg ttccccggaa gcgatttttc tcgccatccc
     4141 aagcacccgc gccggccccg tcgtaaatag ttcgacgagc cgcatcaggg acagcttgcc
     4201 gccatccacc agtcccgcga tgcccaacgc cagcgccgtt tcaaacccag taatgccgaa
     4261 aggcgcgcga tcgaactcga cgtccttgct cgccggatga tgcggcgcgt gatcggtggc
     4321 gatggcatcc acgctgccgt cggctagtcc ttcgatcagc gcatcgcgat cctcgcgtga
     4381 agccagcggc ggattcatct tgtagcgcga atcgtagcgg acatcctcgt ctgtcagcgt
     4441 gaaatgatgc ggcgtcactt cgcacgtcac gcgcaatcct cgcttcttcg ccgagcgcac
     4501 atattccaac gattcgccag tagacatatg cgcgatgtgg agccgcgcgc ccgtcagcgc
     4561 agccacttcc acgtcgcgtc ccgtgcaaat cgattccgat tgcgcgggga tgcctttcag
     4621 ccccaagcga attgactgcg gtccctcccg catcacgccg ccggcaaaaa gtgacgcatc
     4681 ttcgcaatga tcgatcaccg gcaaatcaag cgagttgcag taatccatca cctgccgcat
     4741 caaacgtgca gacgcgatcg gtttcccatc atcgctcacc gccacgatcc ccgcgcgctt
     4801 catcgccgca atttcggcca gcgcttcgcc attgctgccc attgaagccg cgccgat
//
LOCUS       DATNDT010000240         3267 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_291459, whole genome shotgun sequence.
ACCESSION   DATNDT010000240 DATNDT010000000
VERSION     DATNDT010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3267
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291459"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..788)
                     /locus_tag="VN788_07660"
     CDS             complement(<1..788)
                     /locus_tag="VN788_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXU20441.1"
                     /translation="MFKLKARRPLIVKVCILASSSSGNCTFIATSRARILVDAGLSKR
                     ETQARLSAIGESLDSVDAILVSHEHSDHVCGLGAVTRHRDSGIPIYMTSLTARAIDWN
                     DYTPPLESFQAGSSFHIGDIEIDSFTIPHDACDPVGFCFRAGGIKIGVVTDLGYLTDS
                     VKYQLRGTDMLVLESNHDLEMLKVGPYPWPVKQRVMGRRGHLSNEVVGAFLRDELDMR
                     TTTLVLGHLSEHNNHPEIVRLVASQALEGRSLFTRLVIAEPGRQS"
     gene            1078..1464
                     /locus_tag="VN788_07665"
     CDS             1078..1464
                     /locus_tag="VN788_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20442.1"
                     /translation="MRLLTLGIGIAFCFAVLCRAEAIHLNFTDAPGTSVFANPGNREI
                     NEVNLDHLDFTGLTNMPWAQIGGTMDPIMQGGPSFDAAVSIRDPNLSAGSNTNAVSAT
                     PEPITVAMVGSGLILFGMVRPKRRNR"
     gene            1576..2046
                     /gene="ybaK"
                     /locus_tag="VN788_07670"
     CDS             1576..2046
                     /gene="ybaK"
                     /locus_tag="VN788_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HXU20443.1"
                     /translation="MTKTNAIRLLDQAGISYEPREYEADPNDLSAEAVARKIGMPVEQ
                     VFKTLVAEGDRNGVCLAVIPGNLQLDLKALAKCTGDRKIDTVPLKQVQPLTGYIRGGV
                     TALACKKDYPVYIDETAQLFEVISVSAGMRGLQVLLHPEDYRGIVKAAYAEIAR"
     gene            2668..>3267
                     /locus_tag="VN788_07675"
     CDS             2668..>3267
                     /locus_tag="VN788_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU20444.1"
                     /translation="MKSRRKPSLIAFLLAGALSLALTACKSETRTAHAAGFAAPSVQV
                     VEAKPENVPIYSEYVAQTFARDMVEVRGRVDGYIEKRLFQTGQDVRAGETLYVLDRRP
                     YQADVQKAQGDVAQAEANLEFASKQVALAQAQADLAQAQANMVKAQQDVDRLEPLVQQ
                     DAAAKQDLDNAVQALKANQANVKAKEVNVEQIRLSTQTQI"
BASE COUNT          758 a          887 c          968 g          654 t
ORIGIN      
        1 gattggcggc caggctccgc gatgacgagg cgggtgaaca gcgaacgccc ttcgagcgcc
       61 tgactcgcca caagccggac aatttcgggg tggttattgt gctcgctcaa atggcccagc
      121 accagtgtgg tggtgcgcat atccaactcg tcgcgaagaa aggcgccgac cacctcgttc
      181 gacaggtggc cgcggcggcc catgacgcgc tgcttaactg gccaaggata ggggccgact
      241 ttgagcattt cgagatcgtg gttggattcg aggaccagca tgtccgtgcc gcgcaactga
      301 tatttgaccg aatcggtgag atagccgaga tccgtcacca caccgatttt gatgccgccc
      361 gcacggaagc agaagccgac cgggtcgcac gcgtcgtgcg ggattgtaaa actgtcgatc
      421 tcgatatcgc cgatgtgaaa ggacgaacca gcctgaaacg attcgagcgg cggagtgtag
      481 tcgttccaat cgatcgcgcg cgccgtaagc gaagtcatgt aaatcgggat gccgctgtcg
      541 cgatggcggg tgaccgcgcc gagaccgcac acatgatcgg agtgttcgtg gctgacaagg
      601 atggcgtcga ctgaatcgag cgattcgccg atggcgctga ggcgggcctg cgtttcgcgc
      661 ttgctcaaac cggcatcgac gagaatgcgc gcgcggctgg tcgcgatgaa agtgcaattt
      721 cccgaactgc tgctggccag aatacagact ttgacgatta gcggcctccg ggccttgagc
      781 ttaaacaagc aggataggac atggggaggg agaagtctag ggggagaggc gcttgggcgt
      841 cctcaccgtg ctatgcagct ataccagagc agcgccctca tccaatcacg acgagcttcc
      901 tgttttcgga tacgaggacg gtacgcgcaa cttatctccg ctcggacatc gcgtactaga
      961 gtttatgaat cggaagtact gaaagtttta accagtaacc gttcagttct tgcacgcact
     1021 ttgaacggat attagtcttg tgtatgtctt acttttttcg gcggtaggag atcaaagatg
     1081 agactactta cacttggcat cgggatcgcg ttctgcttcg ctgtgttatg ccgggcggag
     1141 gcgatccact tgaattttac tgatgcgccc ggaacgtctg tcttcgctaa cccaggcaat
     1201 cgcgagataa atgaagtcaa cctcgatcac ttggacttta caggactcac gaacatgccg
     1261 tgggctcaga tcggcggaac aatggatcca atcatgcaag gtggaccatc attcgacgcc
     1321 gccgtttcga ttagggatcc taaccttagc gccggctcca acaccaacgc cgtaagcgcc
     1381 acaccggaac caatcacggt ggcgatggtc ggcagtggat tgattctgtt tggcatggtg
     1441 aggccgaagc ggcgaaaccg ttaacagagg ctcgaaacag cgaatgcatc agcaccccgg
     1501 tcagcgcgag ctggccgggc tttttttgcc ggatggcctg acaaaatcgc ggtacaacgg
     1561 atttagcagg ccagcgtgac caagactaac gcaatccgcc ttctcgatca ggcgggcatc
     1621 tcttatgaac cgcgggaata tgaagccgat cccaatgacc ttagcgcgga agccgttgct
     1681 cgcaagattg ggatgcctgt cgagcaggtc ttcaaaaccc tagttgccga aggtgatcgc
     1741 aacggcgtgt gcctcgcggt gattcccggt aatctgcaac tggatctcaa ggcgctggca
     1801 aaatgcaccg gagaccgcaa aatcgatacg gttccactta aacaggttca accgctcacg
     1861 ggttatatcc gcggcggcgt cactgcgctt gcgtgcaaga aggattaccc tgtctacatt
     1921 gacgaaaccg cgcaattatt cgaagtgatt tccgtttccg ctggaatgcg cggtctacaa
     1981 gttctgctcc atccagaaga ttatcgcggc atagtgaagg cagcctatgc ggagatcgcg
     2041 agatagctgt cacatttgga gcgatatgtt ggcaaaaaat atagccagcg tgtcgatccg
     2101 ggcgtgcgtc gctcgaccta ctgatagagg cacattcaga cgaaatacac gttcttgacc
     2161 tacgtagacg aaaagcccgg gcgaggccgg gcagcagcga atcatagccg actgcgccca
     2221 cgtccagcaa ttgatcggta agttttggcg ggcgctccgc tacaccctac atccagagca
     2281 gccatcgttc gcatcgtgac ggtaagctgg tcgtaaccga cagtccgttc gcgcggaagc
     2341 gccgagcaga ccggcgaata cactaccatc cggccggtgg ccgagaaagt gggttttccg
     2401 tcagattagt gcggccggaa gctccggaac ggaaccaaag tccggagggc gaataatatt
     2461 ggttcggaca gattttccaa tgtgccctgt attgcggatg ggatcgacct caaatccgat
     2521 tttggcggta taacgaatag cagagatgca tgtgaacact tgcaacctcc gcgagatcca
     2581 cgcatctaaa ccagcatgtt tccgcccgcc atttttgccg ggccgggcga agttgatgta
     2641 ttgattcgga aaaggaacga gagttgaatg aaatcccgtc gtaaaccgtc cctaatcgcc
     2701 tttctgctcg ccggggcgct ttcgctggcg ctgacagcct gcaaatccga aacacgtacc
     2761 gcgcacgctg ccggattcgc cgctccttcg gttcaggtcg tcgaggcaaa acccgagaat
     2821 gtgccgatct atagcgaata cgttgcgcag acgttcgcgc gcgacatggt ggaagtgcgc
     2881 gggcgcgtgg atggctatat cgaaaagcgc ctattccaaa ccggccagga cgtgagggcc
     2941 ggcgaaactc tgtatgtgct cgaccggcgt ccgtatcagg ccgacgttca gaaggcgcaa
     3001 ggtgacgtcg cgcaggccga ggccaatctc gaatttgcca gcaaacaggt cgcgctggcg
     3061 caagcgcaag cggatctggc gcaggctcaa gcgaatatgg tgaaagctca gcaggacgtc
     3121 gatcgcctgg agcccctggt tcagcaggac gccgcggcca aacaggacct cgataatgcg
     3181 gttcaggcgc tgaaagccaa ccaggcgaac gtaaaggcga aagaggtgaa cgtggagcaa
     3241 attcgcctct cgacgcagac gcagatc
//
LOCUS       DATNDT010000241         5838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3168229, whole genome shotgun sequence.
ACCESSION   DATNDT010000241 DATNDT010000000
VERSION     DATNDT010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3168229"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..466)
                     /locus_tag="VN788_07680"
     CDS             complement(80..466)
                     /locus_tag="VN788_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20445.1"
                     /translation="MKRMIGIAAFIMFFAIPAHAQRGGASSSAAPRMGGGGGIGGSGG
                     GMGNLLPAHPPTHFKMSAVSGSDASFIPSGYLPFESAVAEGQAILDAEKKTPAEAAAA
                     SSIVPKPHAKVAVVQSADGDPIVIQQ"
     gene            complement(761..1648)
                     /locus_tag="VN788_07685"
     CDS             complement(761..1648)
                     /locus_tag="VN788_07685"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HXU20446.1"
                     /translation="MRLKHVFIGSHGKLRAIWRAAIFYAAGEWLVFPLLDRALSLAAR
                     PLHLTQGFTAGNIALSEFANFLTALICTGAFALYERRRVDSYGLPVNRAFSWQTFEGA
                     AAGVIMAGAVAVGMIALGGMQIRGLASSGSALAFSALAWLGANICIGVAEEFWFRSYF
                     QQTLWKSIGFWPATTVIALIFAAEHYFFKQGENVWDVITLVSLSMLLSYTMLRTGALW
                     FAVGFHIAFDYMQLFVIGTPNGSHFPAGRLLDVRFNGPPWLTGGVLGTEASFLMYPVI
                     ALLWLYVWWRYRANPPLQP"
     gene            complement(1781..2989)
                     /locus_tag="VN788_07690"
     CDS             complement(1781..2989)
                     /locus_tag="VN788_07690"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HXU20447.1"
                     /translation="MRLTTLGFWWGFCLSPHAASRLVRANHLRILATPVYTPAACLGG
                     YYSSMIVVRENSRFECLDDLRGSTCAINTPTSHSGMNVLRALFAPMSRNGGFFKSACI
                     TGSHEASLLMIAHGWADVAAIDCITYALLQRRRSRALRGIRVFCCSSRFPAPPYVVGS
                     QVSDAEVDHIRQALVETLATPDMRHLKEALLIKDVVDLPPHSYQSIADLERRALQSEY
                     SEVWDLAGFRRGRAAHIYQMSARKEYDVRKGRLLMKNLFEAATAEEIKERIGRLGPDT
                     QRQWGKMTAAQAMAHCSTTMEWALGDSHEPRMFIGRIVAPLVRSRLLRDEKPMARNTP
                     TAKSLVVKDERDLAKECTRLSALIDRFSEGGPQSCTKHPHTFFGRLTPEEWARLMYKH
                     LDHHLRQFGV"
     gene            complement(2991..3911)
                     /locus_tag="VN788_07695"
     CDS             complement(2991..3911)
                     /locus_tag="VN788_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007295635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXU20448.1"
                     /translation="MNLRNIDLNLLVVLDALLSERNVSRAAQRIGLSQSAMSAALARL
                     REVFHDPLLVRVGRELALTRNAEELTVPVRETLARIEQTLTQRHAFDPKTDARAFSIS
                     ASDYACLVLLTHFVRAIAVEAPSVTIHLLPRSREPARILQTGQADIVIEPSELFGDTK
                     FPSSPLLADRWLCAVDAKNPHIKKKTISRKKFLELPHLVYGIGIDRQLNLADQHLAQL
                     GVQRRIDVTVESFLLVPFLIQGTHLVSLVLERAAKRLAHTTHIRTLEPPFELPDIHER
                     MYWHPRHTTDSGHRWLRERLQSIATDLDHS"
     gene            4128..5738
                     /locus_tag="VN788_07700"
     CDS             4128..5738
                     /locus_tag="VN788_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-hydroxylase N-terminal
                     domain-containing protein"
                     /protein_id="HXU20449.1"
                     /translation="MRTGKEYLESLRDGRKVYVGGELIEDITLHPATKGYAQAIADYY
                     DLHLDPANQDVATFVDWDGKRQSMHWFLPRSKEDLARRRKYTDFLCRHFKGGIFTRPP
                     AGMSVVMFTQVDDQKPWAEGSRFKNGHRDLSGNIQRQWEEVTTKDFAISPMFLDVQFD
                     RSRDNAMAETPMLKIIEERDEGILVRGWKAIGTSVPFVNHLLIGNLWRPGQTPEQTVY
                     ALVPIATKGISVVARQSNAEPDADPYDRPLATIGDELDAMVYFDDVLIPWAQVQHIGN
                     PEHAKWYPQRQFDWVHLETQIRHCVHAELMVGLGLLLTQSLGTSSSPVVQSQLAELIR
                     FRETCRAFMIAAEETGFATPGGLFKANNIYVDFGRAYYLENQHKMVNLLIDFCGRGVV
                     IQPTRKELEDPYIGPKLADALRGPGISAWDRIKIFRQISERFLTEWGSRHEMFEKFNG
                     TPLYLINLLTMQRTEYQVDGPLTELAREVLGFGDTAELGKRAAEAEKESHYANVRYQP
                     KYAQAQDAHTGYIASDSEKDKRTKPAAT"
BASE COUNT         1279 a         1650 c         1768 g         1141 t
ORIGIN      
        1 aaacggccgc cggggagaga atccgggcgg ccgttcttat ttgcaagaca gaggagagtg
       61 ccagggtaat tctctctcct cactgctgta taacgatggg atctccgtcc gcgctctgca
      121 ctacggcgac tttcgcatgc ggcttgggca cgatgctgct ggctgccgcg gcttcagcgg
      181 gggttttctt ttccgcgtca aggatcgcct ggccctcggc tacggcgctt tcaaatggaa
      241 ggtagcccga gggaatgaag ctggcgtccg agccgctaac cgcgctcatt ttgaagtggg
      301 tgggaggatg agcggggagg aggttaccca ttccgccgcc ggatccgcca atgccgcctc
      361 cgccgcccat tcttggcgct gccgaagagc tggcgccgcc cctttgggcg tgagcgggaa
      421 tcgcgaagaa catgatgaaa gctgcaatgc ctatcattcg tttcatggtc acttgcctct
      481 cagcgcgacg ggcgatcact cgacgaacag gagggcaatt gcaaagccag agtggccact
      541 gctctatgtt aaacaaaata tgggcgatag ttggggaggc gaggattcag aggattcgtc
      601 cgaacggcat attgtggaag gatctggcac tcgtgtgagc aaactcgcag agtcccagtg
      661 cttcggtact ggaacttcta aaacgccacc tgatgcaggc ggagggaaga gctacatgat
      721 gcaagatgga aagtgcgacc acccccgacg gtggcgtgaa ttacggttga agaggtgggt
      781 tcgcgcgata gcgccaccaa acatagagcc aaagaagcgc gatcactggg tacatcagga
      841 aactcgcttc agtaccgagc acccctcctg tgagccacgg aggcccattg aagcgcacgt
      901 ctagcaaacg tccggcgggg aagtgtgagc cattgggagt tccgatgacg aaaagctgca
      961 tgtagtcgaa ggcgatatga aaaccgacgg cgaaccacaa ggcgcccgtc cgcaacatgg
     1021 tgtagctcag aagcatgctc aacgagacaa gggtgatgac gtcccagaca ttttcgcctt
     1081 gcttgaagaa atagtgctcc gcggcaaaga tgagcgcgat caccgtggtg gcgggccaga
     1141 aaccgatgct cttccagagc gtctgttgaa aataggatcg gaaccaaaat tcctcggcga
     1201 cgccgataca gatgtttgcg ccgagccagg cgagcgcaga gaaggcgagc gcactgccgc
     1261 tgcttgccag acctctgatt tgcatgccgc ccagggcaat catcccgacg gcaacagcgc
     1321 ccgccatgat gacgccggca gccgcacctt cgaatgtctg ccaactgaaa gcgcgattga
     1381 cgggaaggcc gtagctatcc acgcggcggc gttcgtagag cgcgaaggcg ccagtgcaaa
     1441 tgagggctgt gaggaagttc gcgaactctg acagcgcgat gttgcctgct gtgaatccct
     1501 gggtgagatg taggggtcga gcggcgagtg agagcgcccg atccagcagc ggaaaaacga
     1561 gccactcccc cgccgcgtaa aagatggcgg cgcgccagat agcacggagc ttgccatggg
     1621 acccgatgaa gacgtgtttg agtctcattc cttgctctct cgttgaattc ggcctcggca
     1681 ggtgattcct gggtggtatc atccgcccgg accctcaaat atcaacaagt gaaagccgat
     1741 tgcactaact cctgattaac gcggtaatcg gagaagctcc ctagacgcca aactggcgaa
     1801 gatgatgatc gaggtgcttg tacatcagtc tggcccactc ttcaggcgtt aggcgtccga
     1861 agaaggtgtg cggatgcttg gtgcaactct gcggcccgcc ctctgaaaac cggtcgatca
     1921 acgcggataa ccgcgtgcat tcctttgcca agtctcgttc atccttcacc acaaggctct
     1981 tcgccgttgg cgtattccgc gccatcggtt tttcatcccg tagcaacctg gatcgaacca
     2041 atggcgcgac gatccgtccg ataaacatcc gcggttcgtg gctatcaccc agcgcccact
     2101 ccatagtcgt cgaacagtgc gccatggcct gcgctgcggt catcttgccc cactgccgct
     2161 gcgtatcagg ccccaggcgc ccaatgcgtt ccttgatttc ttcggctgtc gccgcttcaa
     2221 agaggttttt catgaggagc ctccctttgc gaacatcgta ctcttttctg gccgacatct
     2281 gataaatgtg ggccgcccga cctctgcgaa acccggctag atcccagact tctgaatatt
     2341 cggactgcag tgcgcgtcgc tccaggtcgg cgatcgattg ataagagtgc ggcggcaaat
     2401 ccacgacgtc ctttatcagc aaggcctctt ttaggtggcg catatccgga gtagcaagag
     2461 tctcgaccaa cgcctgacgg atgtgatcga cttcggcgtc tgaaacctgg gagccaacta
     2521 catagggtgg cgcaggaaag cgcgaactgc agcagaacac tcgtattcca cggagtgcgc
     2581 gggaacggcg ccgctgcaga agggcatacg taatgcagtc aatggcagca acatcggccc
     2641 atccgtgtgc gatcatcagc aggctggctt catgtgagcc cgtgatgcac gcggacttga
     2701 aaaacccccc gtttcggctc attggggcga atagcgcacg cagcacgttc attcccgaat
     2761 gcgatgtggg cgtgttgatt gcgcacgtcg atcctcgcaa gtcatcgaga cactcgaacc
     2821 gggaattctc tcgaacaacg atcatgctgc tgtagtagcc gccgaggcag gcggcaggtg
     2881 tgtacaccgg cgtcgccagg atgcgcaggt gattcgcccg gacaagccta gaggcggcgt
     2941 gaggcgaaag gcaaaagccc caccaaaagc ccaaggtggt cagtcgcacc tcagctgtgg
     3001 tcgaggtcgg ttgcgatcga ttgcagacgc tcgcgtagcc atctgtgtcc ggaatcggtg
     3061 gtgtgtcgcg ggtgccagta cattctctcg tggatgtcgg gcagctcgaa ggggggctcg
     3121 agagtcctga tatgggtagt gtgggcaaga cgcttcgcgg cccgttcgag gacgagactg
     3181 acgaggtgcg ttccttgaat caagaatggg acgagcagaa aactttccac ggtgacgtcg
     3241 atgcggcgct ggacgccaag ttgagcaagg tgctggtcgg cgaggttcag ttggcggtca
     3301 atgccgatgc cgtagacaag atgcggaagt tccaaaaact tctttcggct aattgtcttc
     3361 ttcttgatgt gcggattttt cgcgtccacg gcacagagcc accggtccgc cagaagcgga
     3421 ctcgatggaa atttggtgtc gccaaacaac tcgctcggct cgatgacaat atcagcctgg
     3481 ccggtctgca gaatgcgcgc gggttctcgc gagcgcggca gaagatggat agtgacgctg
     3541 ggggcctcga cggcgatcgc ccgcacgaaa tgcgtcagca ggacgagaca cgcgtaatcc
     3601 gatgcgctga tcgaaaacgc tcgtgcatcg gtcttcgggt cgaaagcgtg ccgttgcgtc
     3661 agggtctgct cgatgcgggc tagtgtttcc cggacgggca ccgtcaattc ctcggcgttg
     3721 cgtgtgaggg cgagttcgcg acccacacgg acgagaagcg gatcgtgaaa aacctctctg
     3781 agacgggcga gcgcggcgct cattgcggat tggctaaggc ctatgcgttg tgcggcccgg
     3841 ctgacattgc gttcgctgag caacgcgtcg agcaccacga gcaaattcaa atcgatattt
     3901 cggagattca ctgtagcgcc cccctcttcc gctgcggcac ccccgccaga gactatcgcc
     3961 gcaacacgct cagccgcaaa cgatatcggt gagcacgggc gccggcggtt tgccggtgac
     4021 actgatccat cgacggcgtc gatacctgga atcccaaaaa tgcattagac gggccgacgc
     4081 cccggccata tgctctcgat gcgaggaacg gctgaaggag acatgttatg agaacgggga
     4141 aagaatatct ggagtcgttg cgcgacggta ggaaggtgta cgtgggtggc gaactgattg
     4201 aagacatcac ccttcatccc gcgaccaagg gatatgctca ggcaatcgcg gactattacg
     4261 acctccatct tgatccggcg aatcaggatg tggcgacctt tgtggactgg gacggcaagc
     4321 gtcaatcgat gcactggttc ctcccgcggt ccaaggaaga tctggcgcgc cggcgcaagt
     4381 acacggactt tctctgccgg catttcaagg gaggcatttt caccaggccc ccggcgggaa
     4441 tgagcgtggt gatgttcacg caggtcgatg accagaagcc ttgggctgag ggatcccgat
     4501 tcaagaacgg acatcgcgat ctttcgggca acatccagcg ccaatgggaa gaagtgacta
     4561 ccaaagattt tgcaatttct ccgatgttcc tcgacgtgca gttcgatcgc agccgcgata
     4621 acgcgatggc cgaaactccg atgctcaaga tcatcgaaga gagagacgag ggcatccttg
     4681 ttcgcggctg gaaggcgatc ggcacgtcgg tccccttcgt gaatcacctt ctgatcggca
     4741 acctctggcg acccggtcag acgcccgagc agaccgttta cgcgctggtt ccaattgcga
     4801 cgaaaggcat ctcggtggtg gcgcgccagt caaatgccga gcccgacgcc gatccatacg
     4861 atcgtccact ggccacgatt ggcgacgaac tcgacgcgat ggtctatttc gacgatgttc
     4921 taattccatg ggcgcaggtg cagcacatcg gaaatcccga acacgccaag tggtatccgc
     4981 aacgccaatt cgactgggtg catcttgaaa cgcagatccg gcactgcgtg catgcggagc
     5041 tcatggtcgg cctgggcttg ctgctcactc aatcgctcgg tacgagttcc agcccggtgg
     5101 tgcaatcgca gcttgccgaa ctgatccgat tccgcgagac gtgccgagcg ttcatgatcg
     5161 cggcggagga aaccggcttt gccacgcccg gcggcttatt caaggcgaac aatatttacg
     5221 tggatttcgg ccgcgcctac tatctcgaaa accagcacaa gatggtcaac cttctgattg
     5281 atttctgcgg gcgcggcgtg gtgatccagc cgacaaggaa ggaattggag gatccctaca
     5341 tcggcccgaa actcgcggac gcgctgcgtg gccccggcat ttctgcttgg gaccggatca
     5401 aaatattccg tcagatcagc gagcgattcc tgacggaatg gggcagccgg cacgaaatgt
     5461 tcgagaagtt caacggcacg cctctctacc taatcaacct gctaacgatg cagcgcaccg
     5521 agtatcaggt ggacgggccg ctcactgaac tcgctcgtga agtacttggg ttcggggata
     5581 cggcggagct cgggaagcgg gcagccgaag ccgagaaaga atctcactac gccaacgtcc
     5641 gctaccagcc gaagtacgcg caggctcagg acgcgcacac gggctacatc gctagtgatt
     5701 ccgagaagga caagcgtacc aaacccgccg caacgtagta acggacctag tagattcgaa
     5761 gtttcgccaa agggcttcgg tacgcgcccg ccagcccttc cactacgtgc ctcgtctggg
     5821 cgtgacccct aattattg
//
LOCUS       DATNDT010000242         8181 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2598406, whole genome shotgun sequence.
ACCESSION   DATNDT010000242 DATNDT010000000
VERSION     DATNDT010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8181
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2598406"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..722)
                     /locus_tag="VN788_07705"
     CDS             complement(135..722)
                     /locus_tag="VN788_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20450.1"
                     /translation="MWIVFAQCGALPRPLSTVWENVTSTTDPSGNSTLYGYDSSGNVT
                     TQFAPLTDSAAAITAYTYNSFGEVLAMTDPLGNVTSNTYDTHGNLLTVTTPRPNGSTS
                     ASVTHFAYNSLGELTQITDPLNHVTTMTYTPAGLIATITDPQSHVTTYTYDSRGNRTS
                     VTDALSHVTSFTYDAGNRLLSPRTRSYDTRVLLAG"
     gene            925..2295
                     /gene="lysA"
                     /locus_tag="VN788_07710"
     CDS             925..2295
                     /gene="lysA"
                     /locus_tag="VN788_07710"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HXU20451.1"
                     /translation="MTLATISIRKRKIQRRTDPADYTPFFAYQRARAGQNQLLHCEGV
                     AFTSIVDAAGTPTYVYSRASIEAAYRRLDRAFGSLPHRLCYAVKANSNLSVLRVLANL
                     GSGFDIVSGGELDRLRRIGVRGTRIVFSGVGKTREEIREALRYRASRAAPPGILIFNI
                     ESGAELELLLSESARHSSGASSRPSAAIRVNPDVMAGGHPHIATGAHHHKFGIDWPEA
                     RRLYLAYKDSRWLAWRGISAHLGSQIFSLSPYRRALARLSSYFRDLSRNGIRLDHVDI
                     GGGLGIRYTDESPLPPEKYAKALSEIVRPLRCTLLVEPGRTLVGPAGVLLTRVLYVKQ
                     NRGKTFVVVDAAMNDLIRPALYGATHPTTPAVRNRSSIAKNTLVDVVGPVCESGDFFV
                     RDWPLAPVQPGDVLVIWAAGAYCFAQSSNYNARRRAAEVVVEGRRFRVARARETYADL
                     VRGEAR"
     gene            2358..2960
                     /locus_tag="VN788_07715"
     CDS             2358..2960
                     /locus_tag="VN788_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HXU20452.1"
                     /translation="MPLFPAFLKLSGRRCLVIGAGRVAEEKIASLLQAEAEIRVVAPS
                     ATERIRAWSRAGKIRWDARKFRSSDLKNIFLAVAATPSPALHARIFREARRRRVLCNV
                     VDDPAHCDFYYGSVVRRGALQIAISTGGHSPALAQRLRQQFEQEFSNKYERWIDELGA
                     ARRKLFKKTISVARRRALLHRLASQNSFEEFLRRQKRKRK"
     gene            3193..4434
                     /locus_tag="VN788_07720"
     CDS             3193..4434
                     /locus_tag="VN788_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014397994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXU20453.1"
                     /translation="MPATSDTSEKINTRCCIAGGGPAGMMLGFLLARAGIEVVILEKH
                     ADFLRDFRGDTVHPSTLEVLYELGLLEGFLKRKHQEVRQLSGYIGPYRVPIADLSHLS
                     THCKFVALMPQWDFLTFMAESAERYPTFRLQMESEAFDLIEDSGKIRGLRAKTKGGEI
                     EIRADAVVAADGRKSVLRTRAGLEVMQLSAPIDVLWMRISRKPNDPGQVLGRIDAGKF
                     LVMLDRDEYWQCAFLIPKGGLDALRQKGLASFREKVAGIAPFLADRTNELRDWDDIKL
                     LSVAVDRLRQWYRPGFICIGDAAHAMSPVGGVGINLAIQDAVAAANILAEPLRRNSVT
                     ERHLENIQKRRMFPTRATQALQIFIQDRVITPTLKSTTKIQSLPLPLKLLGRMPILRR
                     IPARVIGMGFRPEHVRSPLAT"
     gene            4818..5735
                     /locus_tag="VN788_07725"
     CDS             4818..5735
                     /locus_tag="VN788_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HXU20454.1"
                     /translation="MNIRKGSLHLFRFSGIDVFLHWSWFLVAAYEIQDRATSYSSLSW
                     NALEYLALFLIVMLHEFGHALACRQVGGTSNQIVLWPLGGVAYVDPPQRPGATLWSLA
                     AGPLVNVALLPILYGLELWGRSAGWAETMPNAYHFLYTVVWINLALLIFNMLPIYPLD
                     GGQILRSLLWFGIGRARSLLAATIIGFIGVAGLIGLAVWARSLWFGVLAAFILLNCWS
                     GLQHALALSRVSKLPRREGFACPSCKAAPLMGPYWVCSLCSNGFDTFQTQAVCPNCSA
                     HYATTKCLDCGALHRIDEWSVPEPVPSPL"
     gene            5774..6082
                     /locus_tag="VN788_07730"
     CDS             5774..6082
                     /locus_tag="VN788_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20455.1"
                     /translation="MARTSIAVKKVTATDFRGKLKIYLKEARANRVVLIENRRQPSKY
                     LVDKDFLDSLVKERESIRATLEILADREIVDRLLALSKTIDDEVAVGRLLTTADVFDK
                     "
     gene            complement(6118..>8181)
                     /locus_tag="VN788_07735"
     CDS             complement(6118..>8181)
                     /locus_tag="VN788_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20456.1"
                     /translation="RHRILRPFILSLPFAAAVLFPIPSWSQARTVAGDFTVEPPTLVS
                     LGFQWRISGDDNRNARVDVTFRKKGEQKWRKGLPLLRLQREQVSGGAPRDGGVHYFNY
                     VAPNMFAGSLLNLEPNTEYECRFVLSDPDGVKGKTEKTVAVRTRKEPQPAEGGHVYHV
                     YPFGYKGAKQQPAFTGLLAAYYMGSDQSDHANVMPPRVRPGDIILVHAGLYKDSRFVY
                     GGFDRAIAAYGTPFDGTYYLTQSGTPDKPIVIKSAGDGEVIFDGDGCQNLFNLMAANY
                     NYFEGITVRNTNVAFLLGHKNIGGSSGFTLKHSRLENIGRGVEDDWSGSKNFYIADNV
                     FIGRHDPRKLQSWWTPAVWAKFPGYPAPITSEYAVKIYGQGHVVAYNYVANWHDGIDV
                     ATYGDPDGTPNELPDRVPIDIDFYGNDFYNMADNCIEADGGAHNIRVFRNRCFNSAGG
                     ALSAQPMFGGPLYFYQNLIYNTTTGGPLKLIDTPAGVLIYQNTFFGQGRLMGPASNVH
                     FLNNLILGDGWADPVLNLRTYTNYSSSDYNGFRPNANVANAFEWTSPPFEVAADYQGK
                     LVTRNFKSLKEYSEATKQDQHSVLVDYDIFVNAKIPDKSDPQHFYDPEDFDFRLKPGS
                     TAIDAGILLPTINDDFTGKAPDLGAYELDRPLPHYGPRSWPLGAPANSAPRSVTGPPH
                     QASEP"
BASE COUNT         1628 a         2317 c         2446 g         1790 t
ORIGIN      
        1 cagcatcgag atctgctgat agcgcgtgtg aaaatcacat cctataatct tcattgctcg
       61 gctcctctct tccgagcctt ggtcgctagc gcaaccaagt ttactcggga gaaggagccg
      121 acaacgtcat ctaatcagcc agccaacaag accctggtgt cgtatgatct cgtgcgcgga
      181 ctcaacaacc ggtttccggc atcataggtg aagctggtca cgtgcgagag cgcgtcggtg
      241 accgaagtgc gattgccgcg tgaatcgtag gtataggtcg tgacgtgact ctgcgggtcg
      301 gtgattgtgg cgatcaaccc tgcgggcgtg taggtcatgg tcgtgacgtg attgagcgga
      361 tcagtgattt gcgtcaattc acccagcgag ttgtaagcaa aatgcgtcac gctcgctgac
      421 gtactgccat tcggcctggg agtcgtgacc gtcagcaggt tcccgtgcgt gtcataggtg
      481 ttcgaggtaa cgttccccaa tgggtccgtc atggccagca cttctccaaa gctgttgtac
      541 gtgtatgccg tgatcgcggc agcgctgtcc gtgagcggcg cgaattgagt ggtcacattg
      601 ccagaggagt cgtagccgta gagagttgaa ttgccgcttg gatcggtggt cgaagtgaca
      661 ttttcccaaa ctgtcgaaag cggacgtggt aatgccccgc actgtgcgaa gacgatccac
      721 atgaccgact ttctcttgat ctgcgacaaa aacaacaagg caaatgtcac ggtgcgctct
      781 ttgtaacgcc acgcaacctt ctcatcatta gaatccgagc cgggacacga cccatcgctc
      841 caatgtagct tttcgcgccg gactttgtat gatgctaccc atgatttttg cacgctatcc
      901 agcgcccaac gctcgcgccc caagatgaca ctcgccacga tttcgattcg caagcgcaaa
      961 atccagcgcc gcaccgatcc agccgattac acgccgttct tcgcctacca gcgcgctcga
     1021 gccgggcaaa accagctttt gcattgcgag ggcgtcgcgt tcactagcat cgtcgatgcc
     1081 gctggcacgc caacatacgt gtacagccgc gcgagcatcg aagccgccta tcgccgcctt
     1141 gaccgcgcct tcggctcact cccgcatcgg ctctgctacg cggtgaaagc gaactcaaat
     1201 ctttcggtac tccgtgtcct cgcgaatctc ggaagcggtt ttgacattgt ctcaggcggc
     1261 gaactcgacc gtctgcggcg catcggcgtt cggggaacac gcatcgtctt ttccggcgtc
     1321 ggcaagacgc gcgaggaaat tcgcgaggcc ctccgctatc gcgccagccg cgcggcgccg
     1381 cccgggattc tgattttcaa catcgaatcc ggcgccgagc ttgagttgct cctcagcgaa
     1441 tccgcgcgcc attcgtcggg cgcgagcagc cgcccttctg ccgcgattcg cgtgaacccc
     1501 gacgtcatgg ctggcggtca tccccacatc gccaccggcg cccaccatca caaattcggc
     1561 atcgactggc cggaagcacg ccgcctttat ctcgcgtaca aggattctcg ctggcttgcg
     1621 tggcgcggga tcagtgccca cctcggatcc caaatcttta gcctgtctcc gtatcgccgc
     1681 gccctcgcgc gcctttcatc ctactttcgc gatctatcgc ggaacggcat ccgcctcgat
     1741 catgtcgata ttggcggcgg cctcggcatc cgctacacgg atgagtcgcc tctgccgccg
     1801 gagaaatatg caaaagcgct gtcggaaatc gtgcggccgc tccgctgcac attgctggtt
     1861 gagccgggcc gcacgcttgt cggacccgcc ggcgtgctgc ttacgcgagt tctttacgtg
     1921 aagcagaatc gcgggaagac tttcgtggtt gtagacgcgg ccatgaatga cctgattcgc
     1981 ccggcccttt atggtgctac gcatcccacc acgccggctg ttcgcaatcg ttcgtccatc
     2041 gccaaaaata cattggtgga cgtggtcggc cccgtctgcg agtccggtga ttttttcgtc
     2101 cgcgattggc cgcttgcccc cgttcagccg ggcgatgttc tggtcatctg ggcagcggga
     2161 gcctattgct tcgcgcaatc atcgaattac aatgcgcgcc gccgcgcagc cgaggttgta
     2221 gtcgagggcc gccgcttccg agtggcgcga gcgcgcgaaa cgtatgccga tctcgtccgc
     2281 ggcgaagcca ggtgaagcgc gcgatgcccg tccgcgccac tgacgaatca tcgacgagtc
     2341 agcaagggat ttaagcgatg cctctctttc ccgcttttct gaaactcagc ggacgccgct
     2401 gcctggtgat cggcgcgggg cgagtggccg aggaaaaaat cgcaagcctg cttcaggcgg
     2461 aggcggaaat tcgcgtggta gcgcccagcg caacggaacg cattcgcgcc tggtcgcgcg
     2521 ccggcaaaat ccggtgggac gcgcgcaaat ttcgctcctc cgatttgaag aacattttcc
     2581 tcgcggtagc cgccacgccc tcgcccgccc ttcacgcccg cattttccgc gaggcgcggc
     2641 gccgccgcgt tttgtgcaat gtagtggacg atcccgcgca ttgcgatttc tattacggct
     2701 cggtggtccg ccgcggagcc cttcaaatcg cgatctctac cggcggccat agccccgctc
     2761 tggcgcagcg cttgcgacaa caatttgaac aggagttcag caacaaatat gaacgctgga
     2821 tcgacgagct tggcgccgcc cgccgaaaat tattcaaaaa aacgatttcg gtggcgcggc
     2881 ggcgcgcgct gctgcaccgc ttggcaagcc aaaattcgtt tgaagaattc ctgcgtcgtc
     2941 aaaagcgcaa gcgaaaatga cggtctggag ttcgcgggtt cgcggatgcg cctgggtcgg
     3001 gaccgccccc cttccggggc tcgccggtcg ccgaaccggg gttaaagttg ggccttcctg
     3061 gcatttttcg accctaaaat gtttccagtt agttcaaata tttgcttcaa actagaagct
     3121 ttttgtgcgc ggaaatgcac atcattttta ttcgtccggg ttcaggtaca ctttgagtcg
     3181 atatcaaaga caatgccggc tacatccgat acttccgaga aaataaacac gcggtgctgc
     3241 atcgcgggcg gcggacctgc gggaatgatg ctcggttttc tgctggcccg cgcgggaatc
     3301 gaagtggtga tcctcgaaaa gcatgcggat tttctgcgtg attttcgcgg cgacaccgtg
     3361 cacccctcga cgctggaggt tctgtacgaa ttgggactgc tggaaggatt tctaaaacgg
     3421 aagcaccagg aagttcgcca actatcggga tatatcgggc cttaccgggt cccgatagct
     3481 gatctttcgc acctctcgac gcattgcaaa ttcgttgcgc tgatgccgca gtgggatttt
     3541 ttgactttca tggcggaatc cgcggagcgc tatcccacct ttcgtttgca gatggagtcc
     3601 gaggcattcg atctgatcga agatagcggg aaaatccgag ggcttcgagc aaaaacgaag
     3661 ggcggcgaaa tcgaaattcg tgccgatgca gtagtggccg ctgacggccg caaatccgtg
     3721 ctgcgcacgc gcgcgggcct cgaagtaatg cagctcagcg cgccgattga tgtgctgtgg
     3781 atgcgaattt cgcgcaagcc caacgatccg ggacaggttc tcggccgaat cgatgccgga
     3841 aaattccttg tgatgctgga ccgcgacgaa tattggcagt gcgcgttcct cattcccaag
     3901 ggcggactgg atgcgttgcg ccagaaaggc ctcgcaagct ttcgcgaaaa ggtcgccggt
     3961 attgcgccgt ttctcgccga tcggacgaac gaactaaggg attgggacga catcaaactg
     4021 ttgtccgttg ccgtggaccg gttgcgacag tggtatcgtc cgggattcat atgcatcggc
     4081 gacgcagctc acgcgatgtc tccagtcggc ggcgtgggga tcaacctggc catacaagac
     4141 gcggtggctg ccgccaatat tctcgccgag ccgctccgcc gcaattccgt aacggaacga
     4201 catctcgaaa atattcagaa gcgccgcatg tttcccacac gggctacgca ggcccttcaa
     4261 atattcattc aggatcgagt gatcacgccc acgctaaaaa gcacgactaa aattcagtcg
     4321 ctgccgctgc cgctcaagtt gctcggccga atgccgatcc tgcggcgcat accagcacgg
     4381 gtgatcggaa tgggattccg gcccgagcac gtgaggtcgc ctttggcgac gtagctcgcg
     4441 tagcggtcag gtaagcaagg ctgacgaata ggacccccgg atgggccgca catcacaacg
     4501 gcctcccaca gcttccgctg tgggctacgt tcgtgtcgtc cctgacggga cttggaaccc
     4561 tccgcggctt ccccccaatg ctgacgcagc ggcgtgacgg aatgaatttc cgccgaagat
     4621 cccgcgaggg gctgcatggg accataagtg catggccaaa agctacgttg atctggcgtc
     4681 gtacccggcg gggaagaaac ccgaccttcc gaaagtcaac gttttcatgc gggagcttcg
     4741 tgcagtgagc ttttcgtgcg cgctgtctgg tggtggactg ttcggcgcgt cgcgttgtgc
     4801 tataaatctt atatacgatg aacattcgca aaggttcgct tcacctgttc cgtttttccg
     4861 ggatcgacgt gtttttgcac tggtcgtggt tcctggtggc ggcgtatgaa attcaggacc
     4921 gcgccacaag ctattcctct ctttcctgga atgctctgga atatctcgcg ctatttctga
     4981 tcgtgatgct tcacgagttt gggcacgcgc tggcgtgccg gcaggttggc gggacgtcga
     5041 atcagatcgt gctgtggccg ctggggggcg tggcatacgt cgatcctccg cagcgaccgg
     5101 gtgccacgct ctggagcctc gcggccggac cgctggtgaa tgtggcactc ctgccgattt
     5161 tgtacgggct cgagttatgg ggccggtcgg cgggatgggc ggaaacgatg cccaatgcgt
     5221 accacttttt gtacaccgtc gtgtggatca atctcgcctt gctgattttc aacatgctgc
     5281 cgatctatcc gctggacggc ggtcagattc ttcggtcgct gctttggttc ggcatcgggc
     5341 gcgcgcgcag cctgctggca gcaacgatca tcggcttcat cggcgtcgct ggtctaatcg
     5401 gactagctgt gtgggcgcga tccctctggt tcggggttct cgcggctttt atccttttaa
     5461 attgctggag cggactccaa cacgcgctgg cattatcgcg agtgtcaaag ttgccgcgcc
     5521 gcgaaggttt tgcctgtccg tcgtgcaaag cggcgccgct gatgggcccc tattgggtgt
     5581 gcagcctgtg cagcaacgga ttcgatacgt tccagacgca agccgtctgt cccaattgct
     5641 cggcgcacta tgccacgacg aaatgcctgg actgcggcgc gctgcatcga atagacgagt
     5701 ggagcgttcc ggagcccgta ccttctccgc tgtaattggc cttggtctat agcgttcatt
     5761 agggaggcct ccgatggcac gaacttccat cgctgttaag aaggtcacag ccaccgactt
     5821 tcggggcaaa ctcaaaatat acctgaaaga agcgagagca aaccgggtcg tgttgatcga
     5881 gaaccgccgc cagccttcaa agtacctggt tgacaaagac tttttggatt ccttagtgaa
     5941 ggagcgtgag tcaattcgcg cgactctgga gattctcgcg gatcgggaga tcgtcgaccg
     6001 gttgctggca ctgtcaaaga ccatcgacga tgaagttgca gtaggccggc tgctcacaac
     6061 agcagacgtt tttgataaat gagaaggcgg ccgactgagc cagccagatg ctgatcgcta
     6121 cggttcggag gcttggtgcg gagggccggt gacggacctt ggcgcggagt tagcgggcgc
     6181 gcctagcggc cacgagcgcg ggccgtaatg cggcaatggg cgatccaatt cgtaggcgcc
     6241 gagatcgggg gctttcccag tgaaatcgtc attgatcgtg gggagcagaa tgcccgcgtc
     6301 gatggcggtc gatcccggct ttagcctgaa atcgaaatcc tcgggatcgt agaaatgctg
     6361 cggatcggat ttgtcgggta tcttcgcatt cacgaaaata tcgtagtcca ccaaaacgct
     6421 gtgctggtcc tgcttggtgg cctcgctgta ttcctttagc gatttgaaat tacgggtgac
     6481 gagcttgccc tgatagtccg cggcgacttc gaatggggga gaagtccact caaaagcatt
     6541 ggcgacattt gcatttgggc gaaagccgtt gtaatcggaa gatgaataat tcgtgtaggt
     6601 gcggagattg aggacgggat cggcccagcc gtcgccgaga atcaaattgt tcaagaaatg
     6661 aacgttggag gccgggccca tcaggcggcc ctggccgaaa aaagtatttt ggtaaatcaa
     6721 gacgcctgcg ggcgtatcga tcagtttaag cgggccgcct gtggtcgtgt tgtagatgag
     6781 attttggtag aagtagagcg ggccgccgaa catgggctgt gcgctgagcg cgccaccggc
     6841 cgaattgaag caacgatttc gaaaaacgcg aatgttgtgg gcgcccccat ccgcttcaat
     6901 gcagttgtcc gccatattgt aaaaatcgtt gccgtagaaa tcgatgtcga tcggcactcg
     6961 atccggtaat tcattgggcg tgccatcggg atcgccgtag gtcgcgacgt caatgccgtc
     7021 gtgccagttg gcaacgtagt tgtaggcgac cacgtgtccc tggccgtaga tttttacggc
     7081 gtactccgag gtgatcggcg ccggatatcc tggaaatttc gcccagaccg cgggcgtcca
     7141 ccagctttgc agcttgcgcg ggtcgtgacg accgatgaag acgttgtccg cgatgtagaa
     7201 attcttggag cccgaccaat cgtcttccac gccgcggccg atattttcga ggcgcgaatg
     7261 cttcagggtg aagccgctgg agccgccaat attcttgtga ccgagcagaa aggcgacatt
     7321 ggtattccgc accgtaatgc cttcgaaata gttgtagttg gcagccatca ggttgaaaag
     7381 attctggcaa ccatcgccat cgaaaatcac ttcgccgtct cccgcgctct tgatgacgat
     7441 aggtttgtct ggtgtgccac tctgtgtgag gtaatacgtt ccatcgaaag gcgtgccata
     7501 cgcggcgatg gcgcgatcaa agccgccgta gacgaagcgg ctatctttgt agaggccggc
     7561 gtgcaccaga atgatgtctc cgggacggac gcggggcggc atcacgttag cgtgatcgga
     7621 ttggtcggaa cccatgtagt acgcggcgag caggccggtg aaggcaggct gctgcttggc
     7681 gcctttgtat ccaaacgggt agacgtgata cacgtggccg ccctcggcgg gctgaggttc
     7741 tttgcgcgtg cgcaccgcga cggtcttttc cgttttgccc ttcaccccgt caggatcgga
     7801 aagaacgaag cggcattcgt attcggtatt aggctctaaa ttcagcaggc tgcccgcaaa
     7861 catgttggga gcgacatagt tgaagtagtg aacgccgcca tctcgcggcg ctccgcccga
     7921 aacttgttca cgctgcaggc gcagcagagg cagacctttt cgccattttt gctcgccttt
     7981 tttgcggaag gttacgtcca cgcgagcatt gcgattgtca tctcctgaga ttcgccactg
     8041 gaagccgagg gaaacgagtg tgggcggctc gacggtgaaa tcgcccgcaa cggtgcgcgc
     8101 ttgggaccag gaaggaatcg ggaaaagaac cgccgctgcg aacggaagag aaagaatgaa
     8161 cgggcgcagg atacgatgac g
//
LOCUS       DATNDT010000243         3919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1918829, whole genome shotgun sequence.
ACCESSION   DATNDT010000243 DATNDT010000000
VERSION     DATNDT010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1918829"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..926
                     /locus_tag="VN788_07740"
     CDS             <1..926
                     /locus_tag="VN788_07740"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HXU20457.1"
                     /translation="PGTIRGHAHLGDGAEASIVGALATRHENDLVMPSYRCHGYPLVL
                     GTPARSLMAEIYQRKDGICKGFGGSMHLADPAHHFPTASGIIGQGIAHAAGAGYAAQV
                     KKTGQVVYSFFGDGSSKQGAFHETLNLAAVWKLPVVFVLENNGYQAYTHVALEDANAM
                     AGDPLSQKAKAFSIPGETVDGTDVLKVYEVVGRAAERARAGQGPSLVESKFYRFSPHG
                     NAITVPPVPTQFQEHESIEVYGRKPEFEAAQKNDPIAKFRAVLIGNGTFAEAEAKRIE
                     DGVRQEMEEVVKFALASPLPAPEDALNYVWA"
     gene            952..1932
                     /locus_tag="VN788_07745"
     CDS             952..1932
                     /locus_tag="VN788_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007931606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HXU20458.1"
                     /translation="MPREMMFAEAIAVAITEEMQRDPNVVFYGQNMAMTDRDPFVKKF
                     GKDRVRITPISETAELGIAIGAAMSGLRPVVELYMSEFMLVAFDEVVNEAPRLRYMSG
                     GQVQVPVVFKAGFGFAAGWAGQHSNCIYSMMMGIPGLKVALPSTAADALGLMKSAIRD
                     NNPVVYLHHYMLMLDKSPVPDGDHLVPFGVADVKRAGKDVTIVATAWMVKKSLLAAEM
                     LAKEGISAEVIDPRTVAPLDVKTIVESVKKTGRLVLVDQAPRQSSAAVVIAGEIADQG
                     FKYLKAPVKMVTALDTSVPYSEPMESFVLPSEQKIVDAVKSVLAVHAAAV"
     gene            2167..2988
                     /locus_tag="VN788_07750"
     CDS             2167..2988
                     /locus_tag="VN788_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20459.1"
                     /translation="MRNSRFGIVLFSAGLIVSSALTFVSAAAAQNTFKKAVPTAADWA
                     AVAKLPDFTGVWEIPLGGSTPRATRSGAALSPRRPALSLTPEYEAKSKALMANAQQDN
                     QTANCLPPGMPGIMGQPYPMEFLLTPGQVTIIIEAYSETRHIYTDGRPLPDDPDPTFF
                     GTSIGHWEGDTLVVQTVGFSTQTEIEPHVPHSDKMSILEKFRLLDPNTMSIETTVTDP
                     EALAAPYVNKRILKRHRNWTIAEYICEQNNRNFVDQNGKAGINLTVPKDATPAPK"
     gene            3024..3443
                     /locus_tag="VN788_07755"
     CDS             3024..3443
                     /locus_tag="VN788_07755"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXU20460.1"
                     /translation="MKPITKYAILAAILFSGVAAATRTAAAAPAHHGFAVFDTSAQKT
                     ITGTVREFQWTNPHTWVWLDVPNGKGGSDTYGFEGMSPNYLERRGWTRKTLKPGDTIT
                     VTYFPLKTGAHGGSFLIGKRPNGEELRMFINSPTEDH"
     gene            3642..>3919
                     /locus_tag="VN788_07760"
     CDS             3642..>3919
                     /locus_tag="VN788_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HXU20461.1"
                     /translation="MSTPAAIPKTTELRLAHSPDSDDAFMFYALATGKVRLPGVKFSH
                     TLSDIESLNKAAHEEKYDVTAVSFYGYAFVADKYVLLDCGASFGEGYGP"
BASE COUNT          883 a         1225 c         1060 g          751 t
ORIGIN      
        1 atcccggcac gattcgcggc cacgcgcatc tcggcgacgg cgcggaagcc agcattgttg
       61 gcgcgctggc cacacgccac gaaaacgatc tggtaatgcc cagctatcgc tgccacggtt
      121 acccgctggt tcttggcacg cctgcccgct cgctgatggc tgaaatttat cagcgaaaag
      181 acggcatctg caaaggcttc ggcggctcga tgcacctcgc tgatccggcg catcactttc
      241 cgaccgcctc cggcatcatc ggacagggaa tcgcgcacgc cgctggggct ggctacgcag
      301 cgcaagtgaa aaagacaggc caagtcgttt attctttctt tggcgacggc tcatcgaagc
      361 agggcgcgtt ccacgaaacg ctgaatctcg ccgccgtttg gaaacttccc gtcgttttcg
      421 tgctcgaaaa taacggatac caagcctaca cgcacgtcgc actggaagac gccaacgcca
      481 tggccggcga tccgctctcg caaaaagcaa aagccttcag cattcccggc gagacggtcg
      541 atggcaccga tgtcctcaaa gtttacgaag tagttggaag ggccgccgag cgcgcgcggg
      601 caggacaagg cccgagtctc gtcgaatcga agttttaccg gttcagcccg cacggaaacg
      661 ccatcaccgt tcctcccgtg cccacgcaat ttcaggaaca cgagtccatc gaggtgtacg
      721 gcaggaaacc cgaattcgag gccgctcaga aaaacgatcc catcgccaag ttccgcgccg
      781 tcctaatcgg caacggcact ttcgcggaag ccgaagcgaa gcgaattgaa gacggagttc
      841 gccaggaaat ggaagaagtg gtgaagttcg cactcgccag tcccttgccg gcgcccgaag
      901 acgccctgaa ttacgtctgg gcataacgcc gcatagaaag aaggagccat catgccgcgc
      961 gaaatgatgt ttgccgaagc gattgccgtg gccatcacgg aagaaatgca gcgcgatccc
     1021 aacgtcgttt tctatggcca gaacatggcc atgacggatc gcgatccatt cgtcaaaaaa
     1081 ttcggcaagg atcgcgtgcg catcactcca atttcagaaa ccgccgaact cggcatcgct
     1141 atcggcgcgg ccatgtccgg cctgcggccc gtcgtcgaac tgtacatgag cgaattcatg
     1201 cttgtggcat tcgacgaagt cgttaacgaa gctccgcgcc tgcgctatat gtccggcggt
     1261 caggtacagg ttccggtcgt tttcaaagcc ggattcggat tcgccgccgg ctgggccggc
     1321 cagcactcga attgcattta cagcatgatg atgggcattc ccggcctgaa agtcgcgctt
     1381 ccatcgaccg ccgccgacgc gctcggcctg atgaaatccg cgattcgcga caataatccc
     1441 gtggtctatt tgcaccacta catgctgatg ctcgataaaa gccccgtgcc cgatggcgat
     1501 cacctcgttc ccttcggcgt agcggacgta aaacgcgctg gcaaggacgt caccatcgtt
     1561 gccacggcgt ggatggtgaa aaaatccctg ctggccgccg aaatgctcgc gaaggaaggc
     1621 atcagcgccg aagtaatcga tccgcgcacc gtcgcgccgc tcgacgtgaa aaccatcgtc
     1681 gaatccgtga agaaaaccgg ccggctcgtc ctggtcgatc aagccccgcg ccaatcctcg
     1741 gccgccgtcg tcattgccgg agaaatcgcc gatcagggat tcaaatatct caaggcgccg
     1801 gtaaaaatgg tcacggctct cgatacttcg gtcccgtaca gcgaaccgat ggaaagtttc
     1861 gttctgccca gtgaacagaa aatcgtagat gcggtaaaat cggtgctggc ggtccacgcc
     1921 gccgctgttt gatcgaaatt gcagtaacct taggggcgtt gtcgccgtcc gaatcagggt
     1981 agagcaatcg tagtcgcgct tgttcttcgt aggggcacag catgctgtgc ccggcgcaca
     2041 tgcttggcgt gaagtctgct atcggcctcg cgccaacggc cgcgcactgt gttcgctgta
     2101 gggacgctgc cttgccctga gcgttttccg cgaagggttg ctgcgcccgc ctttggagga
     2161 accgcgttgc gcaatagtcg atttggaatc gttctcttca gcgcgggttt aattgtctct
     2221 tccgccctca cgttcgtgtc tgccgccgca gctcagaata cgttcaaaaa agcggtaccc
     2281 accgccgccg attgggctgc cgtcgccaaa ctcccggact tcaccggcgt ttgggaaatt
     2341 cccttgggcg gatccacccc ccgcgccaca aggtccggag ccgccctatc gccccgacgg
     2401 cccgcgctct cgctcacgcc cgaatacgag gccaaaagca aagctttgat ggctaacgcg
     2461 cagcaggaca accaaacggc caactgcctt ccacccggca tgcccggaat catgggacag
     2521 ccgtatccaa tggaatttct gcttacaccc ggccaggtaa ccatcatcat cgaggcgtat
     2581 tccgagacgc gccacatcta caccgatggc cgccctttgc ccgatgatcc cgatccaact
     2641 tttttcggca cttccatcgg ccactgggaa ggcgatacgc ttgtcgtcca aaccgtcggc
     2701 ttttctactc agacggaaat cgagccgcat gttcctcaca gcgacaagat gagcatcctc
     2761 gaaaaattcc gcctcctcga cccgaacacc atgagcatcg aaaccaccgt caccgatcct
     2821 gaggcactcg ccgctccgta cgtaaacaaa agaatcctga aacggcatcg caattggact
     2881 attgcggaat acatttgcga gcagaataat cgaaatttcg tggaccagaa cggcaaggcg
     2941 ggaatcaatc tcacggttcc aaaggacgcg acgcccgcgc caaagtaaaa ttgccacact
     3001 cgaattgcgg aggctccaag agcatgaaac caataaccaa gtacgcaatc ctggcggcaa
     3061 tcctattttc cggcgtggct gcagcaacaa gaacggcggc cgctgcgcct gcgcatcacg
     3121 gcttcgccgt attcgacacc tccgcacaaa aaaccatcac cggcactgtg cgcgagttcc
     3181 agtggacgaa ccctcacacc tgggtttggc tggacgtgcc caatggcaag ggcggctccg
     3241 atacctacgg cttcgaagga atgagcccca attatctgga gcgccgcggc tggacgcgca
     3301 aaaccctgaa gcccggagat acaatcaccg tcacctattt cccgctcaag actggcgcgc
     3361 atggcggcag cttcctgatt ggcaagcgcc ccaacgggga agaactacgc atgttcataa
     3421 actcccccac cgaagaccac tagccgatca aggaagctct gcaagcaccc gggcaaaggg
     3481 taggcgagtc ccggcccggt atggtatcct ctgttggttt tcatactcac gttcgcaggg
     3541 cgaggcacac cacacgcagg ccacttttca aattttctga cgggccgcgc cactgtgtgt
     3601 ctggcgtccg cgcacgatga gcattgagga tggtcaccaa aatgtcgacc ccagccgcga
     3661 taccgaagac aaccgagttg cgcctcgcgc actcgcctga tagcgacgac gcgttcatgt
     3721 tttacgcgct cgccacgggt aaagtgcgcc ttcccggagt gaaattctcg cacactctct
     3781 ccgatatcga atctctcaat aaggctgcgc acgaggaaaa gtacgatgtc accgccgtca
     3841 gcttttacgg ctacgcattt gtggcggata aatatgtgct gctcgattgc ggcgccagtt
     3901 tcggcgaagg ctatggacc
//
LOCUS       DATNDT010000244         5874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3612167, whole genome shotgun sequence.
ACCESSION   DATNDT010000244 DATNDT010000000
VERSION     DATNDT010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5874
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3612167"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..452
                     /locus_tag="VN788_07765"
     CDS             96..452
                     /locus_tag="VN788_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU20462.1"
                     /translation="MLKSDADTELLSAVDHARHRQPFFSGKLAVSMAESFKQVSSEAG
                     APVHKDLEGSPLTNREIQVIQLLADGKSNKQVADTLGVSTRTIESHRNHIMRKMGFTS
                     FSDLIRFAVRTNLIKP"
     gene            complement(790..1002)
                     /locus_tag="VN788_07770"
     CDS             complement(790..1002)
                     /locus_tag="VN788_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20463.1"
                     /translation="MQILSEGQTIRHTQYGLGTVTESNNERTSVDFDDHGMKKFVTSI
                     WNAELIGEPPADRPVRRRGRRKTVKK"
     gene            complement(1090..1320)
                     /locus_tag="VN788_07775"
     CDS             complement(1090..1320)
                     /locus_tag="VN788_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003657892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HXU20464.1"
                     /translation="MNKLGEFELATEQGTVKWFNAAKGYGFIQRQSGEDVFVHFSAIQ
                     SEGYKSLNEGQAVEFEVKQGPKGLQAERVVGL"
     gene            1803..2654
                     /locus_tag="VN788_07780"
     CDS             1803..2654
                     /locus_tag="VN788_07780"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXU20465.1"
                     /translation="MTRHPDLIYDVGLFDGGDSAYYLARGYRVVAIDANPSMVERARS
                     RFKADVEAKRLNLLNVGIAKSPGTATFWVSDKPEWSSFNREIASRDDTHHRPVPVPVV
                     PLSDLLSEYGIPHYLKIDIEGNDRLCVESLKGAELPQYVSVEAECVGDSDRLSDEEAV
                     GTLESLREVGYRKFKLVSQEGWRPVRPRGAGRFFMRLVDSAAGGRLRVKGVSKIARRF
                     TDYGRIAALGYRFAPDSTGPWGEDVPGKWMPFEKAKSVYLETRRSHFSDSEQPLYSFW
                     YDWHATY"
     gene            complement(3016..3255)
                     /locus_tag="VN788_07785"
     CDS             complement(3016..3255)
                     /locus_tag="VN788_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20466.1"
                     /translation="MRTAKSFTIDEDVDEYVSATKGNGSASERVNELLRRAILQEQAE
                     HLEREAAAFFAKPRNAGRKEARAYQKAAMRTLARH"
     gene            3755..4402
                     /locus_tag="VN788_07790"
     CDS             3755..4402
                     /locus_tag="VN788_07790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU20467.1"
                     /translation="MFLALAVGLVLAASVARADSVSFTLGTGNSDISGFPGPYGTATV
                     TLLTPTTASVDFLADGGFEFGGVSSVDVNVNATSWTLGLISVANTGTFFTPGPATDGG
                     SGNVNGFGTFNQTINSFDGYTHGETEVQFTLTDTSGTWANAASVLTANSAGNTVAAHI
                     FVCASDICDSSVDALATGFATNGPGEQKVPEPSSLSMLGLGAMGLLGFARRRMAG"
     gene            complement(4657..5697)
                     /locus_tag="VN788_07795"
     CDS             complement(4657..5697)
                     /locus_tag="VN788_07795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXU20468.1"
                     /translation="MAAEKPYKLSVLDVSPVPSGSTSADALRNTLDLAQLADRLGYTR
                     YWLAEHHNTPLIASSTPEVMIGHVAAVTKNIRVGSGGIMLPNHTPLKVAETFRVLEAL
                     HPGRVDLGLGRAPGTDPLTALALRRTRQALDADDFPQRLEDLLAFLNHGFPSNHPFER
                     IRAMPDDVPMPEIWLLGSSDFSAMLAAQMGLRFAFAHHIQPSPAIAALRLYHQNFEPS
                     EYLKKPESLLGVSVVCAETDERARELARPLELTLLRFRTGRMGRFPTIEEAANYPYSP
                     EERAIIEMNRERSFVGSPSTIREKLTRLAGEGGVNEIMITTMTHDHADRRRSYELLAE
                     AFSLTPAPASSF"
BASE COUNT         1212 a         1583 c         1841 g         1238 t
ORIGIN      
        1 cggatctgat ggtgctggac cttaccatgc ccgaaatgtg aacgggctgg aggtggcgcg
       61 agtggctcgc gaagagtcgc ggagccatcg gttacgtgct caagtccgat gcggatacgg
      121 agctgctctc cgcagtggac cacgcccggc accgccagcc cttcttcagc ggcaagctgg
      181 ccgtgtcgat ggccgaaagc ttcaaacagg tctccagcga agcgggagcg cccgtacata
      241 aggacttgga aggcagcccg ctgacgaatc gcgagataca ggtgattcaa ctgctggcag
      301 atggaaaaag caacaaacag gtggcggata cgctgggcgt ttccacgcgc accatcgaaa
      361 gccaccgcaa ccacatcatg cgcaaaatgg gatttacgag cttcagcgat ttgattcgct
      421 tcgccgtgcg caccaacctg ataaagccgt agatacgacc tcgggcctgg actcaacgat
      481 cctgtagcgc cggcctgccg gcgttttctc cgcggtgttc gaaaggcaaa ttcgccgcca
      541 agatgcgctt cgaggctccc ttcctgcgtc agggcaggca gggcaaggcg gcgctacaag
      601 agctgcgagg cgataccgaa caaatagcca agcatgcttg ccgggcgtag cagtgctaca
      661 cccctacgaa agacatgaaa gcggccgctg ggaatcctgg tgctgtgacc tgcgaatgaa
      721 gaagcaaacg gtggcgccta gcgagaccgg ggcgcactcc gaccaaatga aggtgaagtc
      781 cggaaactac tacttcttca cggttttgcg gcggccgcgg cggcggacgg ggcggtcggc
      841 gggtggctct ccaatcagct cggcgttcca aatcgaggtg acgaacttct tcatgccgtg
      901 atcgtcgaaa tcgacgctgg tgcgctcgtt atttgactcg gtgacggtgc ccaggccata
      961 ctgcgtgtgg cggatagtct gcccttcgga caaaatctgc acaatgcctc cttcaaaaca
     1021 cttatgatcg acctatgatc gcgagcggcg catgcccggc gccgccttaa agcttatgta
     1081 acgtgctgct tagagcccta cgacgcgctc agcttgcagc cccttggggc cctgcttcac
     1141 ttcgaattca acggcctgcc cttcgttgag gcttttgtac ccttccgact ggatcgccga
     1201 gaagtggaca aaaacgtctt cgccggactg ccgttggatg aaaccataac cttttgctgc
     1261 gttaaaccac ttgactgttc cttgttctgt agccaattca aattctccta atttattcat
     1321 tcggcggctt ttaatcgcca aatggtcaat tgtgttcagg ggcgttccgg cgaacacaaa
     1381 aaaaggctgt cggaagttgc ccggcagcct gaagcgagcg cgtcgatatc gaccccaaga
     1441 gcaacaaatg caaagctata gtagcacaag cgtgggcgaa atacaacagg cgggtaatca
     1501 caacagcgtc aggcgggacc tgagaattcg gtgtgaaatt gccgcgagct aggttgaaaa
     1561 gagatcgtcg attgcgctcg tcggcgttcg tcgcgaaagg gcaacggcgt gccctgagat
     1621 cgagaccgac ggttacatcg gggccaagta tttttgccgg gcacggcatg ctctgccgcc
     1681 gcgaagaaac gtcgcgacca tcgccaatcg caagtgaagt tcagtcgcag caagaggata
     1741 cagattttta cgtggttttc ttttgtcctg actcacggta ttatcggctg ccaattgctt
     1801 cgatgactcg ccaccccgac cttatttacg atgttggcct tttcgatgga ggagattcag
     1861 cctactacct tgctcgcgga tacagggtgg tggcgatcga cgcgaatccg tcgatggtgg
     1921 agagggcaag aagccgattc aaggcagacg tcgaggccaa gcgcctgaac ctgctgaatg
     1981 ttggtattgc gaagtctccc ggaacagcca ccttctgggt ttccgacaag ccggaatgga
     2041 gttcctttaa ccgggagatt gcttcacgag acgacaccca ccatcggccg gtcccagtgc
     2101 cggtggtgcc gctctcagat ctgctctcgg agtacggcat acctcactat ctaaaaattg
     2161 atattgaggg aaacgacagg ctgtgcgtcg agagtctcaa aggagcggaa ttgccgcagt
     2221 atgtgtccgt ggaagcggag tgcgtgggag attccgaccg cctctcggac gaggaagcgg
     2281 tggggacgct ggagtcgcta cgagaggttg gctacagaaa gttcaagctc gtgtcccaag
     2341 agggatggcg gcccgtgcgt cccaggggcg cgggacgctt ctttatgcgg ctggttgaca
     2401 gcgcggccgg agggcgcctt cgcgtaaagg gcgtctcgaa aatcgcccgt agatttacgg
     2461 actatggacg cattgcggcg ctgggctatc gtttcgctcc ggattccact ggtccatggg
     2521 gcgaggacgt ccccggcaag tggatgccat ttgaaaaagc gaagtcggtt tatctcgaga
     2581 ctcggcggtc acatttttca gattccgagc aaccgctgta ttcattctgg tacgactggc
     2641 acgccacgta ttagcgtccg cttcggtttc cttaaatcag cccaagagca atccttactg
     2701 cggcctcgat agactggaat gtttgcgggg ataagaagcc taaagcggga tagcgcaggt
     2761 aggatttttc caacgtggtc acttgatcgc acttcgccca acaattccga tcgaggccgc
     2821 cttcgtgcgc gcgaatggga atccgcatgc tgaattcgcc atgttggacg gaggtaaccg
     2881 gaaccacgca aacgtccaag gcgaatttgt tcaacgaatc gaccgacaag atgaccacgg
     2941 ggcgcggctt atctagccgc gcgacataaa cctctccctt tttcgggtgc gatctcgcgg
     3001 atgggtgcga cggcattagt gcctggcgag cgttcgcatc gcggccttct ggtaggctcg
     3061 ggcttctttg cggcccgcgt ttcggggttt cgcgaagaag gctgcagcct cgcgttcaag
     3121 gtgttccgcc tgctcctgca atatggcgcg gcggagcaat tcattcacgc gctcgctggc
     3181 ggatccgttg cccttggtgg ctgagacgta ttcgtccacg tcttcatcga tggtgaagct
     3241 cttagcagtt ctcatagtaa atacagtata tcacatatga tacttctcaa cgaagctcca
     3301 tggacccggc tcgaaaagtt tgctgaggta aatcgaagag ccgccaggaa tgcccctcgg
     3361 cttggcggcg ctacacgtgt cccgagccat agcagccgag cgtgtttgcc cttcgcccac
     3421 cacgttcaaa caggccggac atcggttgtg gcggctgtgc cccgcggaga aaacgccggg
     3481 ctttcgactc cgctcgttgg ctggcaatcc gaaaggaagc cagattagcc ctacgtctct
     3541 ttatttgtac aaaagccttg cgggttgccg caactgcgca aagccttgca catgctgtga
     3601 ctcataggac cactccgcta ccgcgcataa ggcgtttgga tgcaatcgcc aattaacggc
     3661 agcgagcgga caaagtttgg cactacgagt gcagtttcgg gcgccacaag aagtagacgg
     3721 ttgatggtct agaggtgtac ctatgagaaa attcatgttc ctagcactgg cagtggggct
     3781 ggtgttggca gcttctgtgg cgcgggccga ttcggttagc tttaccctcg gcacaggtaa
     3841 cagtgatatt tcgggatttc ctggtccgta cggcaccgcg accgtgacct tgttgactcc
     3901 cacgacggct tcggtcgatt tcctggctga tgggggcttc gaattcgggg gcgtcagcag
     3961 tgtcgacgtg aacgtcaacg caacaagttg gacactcggt ctcatcagtg tggccaacac
     4021 tggcaccttc ttcacacccg gcccggctac ggatgggggc tcggggaatg tgaacggctt
     4081 cggtaccttc aatcaaacaa taaacagctt cgacgggtac acgcatggag agacggaggt
     4141 ccagttcacg ctgaccgaca caagcgggac gtgggcgaac gctgcctccg tacttactgc
     4201 gaactccgct ggcaacaccg tcgcagcgca tatcttcgtt tgcgcttcgg atatttgcga
     4261 ttcctcggtg gatgctctcg ctactgggtt cgccacgaac ggtccggggg agcagaaggt
     4321 tccggagcca agttcgctga gcatgctcgg gttgggcgct atgggtctgt tgggctttgc
     4381 tcgtcggcgg atggcgggat agctcgccaa aacaatcacg ttagttaaca aattcaaagg
     4441 actctgggtt gcgcacgccc cagagtcctt tttttggttt taggggagtc tcgcgtggtt
     4501 ttgatgtcac gcggttttcg cgggtgcgaa acggatgcga aggatctttc tttggctcac
     4561 gctgagacac ggcgtacatc caagagagat tcttcgactg cgtgactggg cgtcccgcag
     4621 gagcgggacg aaaaaaaaag cgcccagcca ctccgctcag aatgacgacg cgggggccgg
     4681 ggtcagggag aaggcttcgg cgaggagctc gtacgagcgg cggcggtcgg cgtggtcgtg
     4741 ggtcatggtg gtaatcatta tttcattgac gccgccctcg ccggccaggc gcgtgagttt
     4801 ttcgcggatg gtcgagggcg agcctacgaa ggagcgttcg cgattcattt cgatgatggc
     4861 gcgttcttcg ggggagtatg ggtagttggc ggcttcttcg atggttggga agcggcccat
     4921 gcgaccggtg cggaagcgca gcagagttag ttcgaggggc cgcgccagtt cccttgcccg
     4981 ctcgtctgtt tcagcgcaga cgacggagac accgagcaga gattccggtt tcttcagata
     5041 ttccgacggc tcgaaattct gatgatagag gcgcagcgcg gcgatggccg gcgacggttg
     5101 aatgtggtgc gcgaaggcga agcgaagtcc catctgcgcg gcgagcatgg ccgagaaatc
     5161 gctggagccg agcagccaga tttcgggcat gggaacgtcg tcgggcatgg cgcgaatgcg
     5221 ttcgaaggga tgattcgagg ggaagccgtg attgagaaac gcgagcagat cttcgaggcg
     5281 ctgcggaaaa tcgtcggcgt cgagggcctg gcgcgtgcgg cgcagcgcga gagcggtgag
     5341 cggatcggtg ccgggcgcgc gtccgagtcc gagatcgacg cggccgggat gcagcgcctc
     5401 gaggacgcga aacgtctccg caactttcag cggcgtgtga ttcggcagca tgatgccgcc
     5461 cgagcctacg cgaatgtttt ttgtgacggc ggcaacgtgg ccaatcatca cttcgggtgt
     5521 cgagctggcg atcagcggcg tgttgtggtg ctcggcaagc cagtagcgcg tgtagcccag
     5581 gcgatcggca agctgcgcga ggtcgagcgt gttgcgcagg gcgtcggcgc tggtcgagcc
     5641 ggaaggtacc ggcgaaacat ccagcacgga taatttgtac ggcttctcag cggccatcga
     5701 ttccctgttc gtctccctgt tcagcgcgcg gcgcgcgtgc ttgcgcaatt cattgttgca
     5761 cacgcacgcg gagtggtgaa tggaatcaga tgatttcagg cgagcggtcg atgcagatgg
     5821 atgtagtgcg aggcggcgat caacgcgtgc acagcagcga ggcttgacgc taat
//
LOCUS       DATNDT010000245        10177 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1484984, whole genome shotgun sequence.
ACCESSION   DATNDT010000245 DATNDT010000000
VERSION     DATNDT010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10177
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1484984"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1175)
                     /locus_tag="VN788_07800"
     CDS             complement(<1..1175)
                     /locus_tag="VN788_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HXU20469.1"
                     /translation="MPIPTLAEKLQWLKPAPATPNEIECAAKVRQGDYEIGVQITNDI
                     LDEAMEVFVRASRSYFGVSGDSMVAIFTANGDLINAACGTYLHAIIQPIIIKFIRKYY
                     ETNPGIRDGDVWYTNDALYGGIHNPDQVALMPIFHQGKLIAWSSAALHTTETGATEPG
                     GMPVSAKSRFEEGLNLPPIKIGENFEIRQDFLEMYSAYGMRAPAMFISDLRARCTTAD
                     RVRVRLLELADKRGADFVIGLLRKMSDTAEAGARKIVKELPDGKFRTVVFNDAIGWTP
                     ALVRSCYLTLTKKDGGIIFDFHGTSPENPSSYNVHPQAVVGHIANFMYEYMFHDLPIC
                     SSTFAPLDFVFPEGSIVYPDKMAATSCCVYIGMQARCATQNSFAKMIFSNAALWRQVA
                     "
     gene            complement(1191..3320)
                     /locus_tag="VN788_07805"
     CDS             complement(1191..3320)
                     /locus_tag="VN788_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011237168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXU20470.1"
                     /translation="MNSIDIDVGGTFTDLVLNFDGKALIKKVPTTPYDLSVCFSKVIE
                     EGAGTLGMQIDQLLPAIDMIRYSTTIAMNRLIERKGPRLALITTEGHEDVVLIGRGAQ
                     WIDGTRVQERRNLAIQSKPHPLIPRDLIVGVKERVDSRGTVIRPLDENNVREKVRYLV
                     SRGARGFVVSLLWGFLNPIHERRVKEIIRDEYKEFHIGYMPVVLAGQVVGKLGEYERT
                     LAAILDAYLQRSMQIELSAMWDKLRERGYKKPLLMIQSSGGIAEVFRTSASRTFNSGP
                     VSGLMGAHHVAKSLGYQNVVMTDMGGTSFDVGLVVKDSVRSYDFRPIIDRWMVGITMI
                     KTLSIGAGGGSIASVNRLLQNRVQVGPRSAGSMPGPACFNLGGVEPTVTDADVVLGYI
                     NPDYYFGGKMRIDKNRAVQAIRDKVAKPLGISVEEGAALIRKIVNGNMSSAIMKEVHL
                     RGYSPEDFILFVGGGAGATHAEGFKGDIQKAVLFPFSPVFCAYGSSTMDIMHVYEASK
                     KITLMTPGDQQLTHDYAAYNATVEALLEQARKDLSADGVDPKTAAYVLELDMLYGGQF
                     HVKRALSPRLSLHSDEDIRAVCDAFNKEFSEAFSPFVVNPEGGIFIESFILKAIVTTP
                     KVHLPKLPLEGKDPSGARKGERPAYWPEEKTFRPTPTFTYESLRPGNVVDGPAIVEGQ
                     YTTMVVPPGMRFSIDERGLGILEAAGS"
     gene            complement(3317..3700)
                     /locus_tag="VN788_07810"
     CDS             complement(3317..3700)
                     /locus_tag="VN788_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013677933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetone carboxylase subunit gamma"
                     /protein_id="HXU20471.1"
                     /translation="MAEGKNQIRITEYLDLDLETERWMCNRCGHVLGPARENYKKGCL
                     LYDRDPREIHPQVVSASFNFAPDPNWVRIVECYCPGCGTQIETEYLPPGHPITHDIEI
                     DLDSLKERVRKGEFTIREKRLEAAE"
     gene            complement(3713..5761)
                     /locus_tag="VN788_07815"
     CDS             complement(3713..5761)
                     /locus_tag="VN788_07815"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HXU20472.1"
                     /translation="MLRYGSLITPRDWNGQFLVWTVDIDVGGTLTDGLFSSGDKVVSV
                     KVDTTPHDLTVCLFDCLAQGAEKLGFADTASFLENVDLIRWSTTITSNVLAELRGPRI
                     GLLVSEGRQKNLYGNGAESAVLNRLLSSRDVIGLDGSANETAIMNAARLLLESGVRRI
                     CISLEDSHRHPEREISWKRTIDQQYPDHFLGSVPVLAGSDISKSSDDRTRAFCAVINA
                     YTHGALAATLFKAEDDLRETSHYAGTFLVSHINGGVAGIAKTRALDTLESGPVLGILG
                     SANQAKARGLKDVIAMDVGGTTAKISVLADGEPMYRKPSDLFGIPVELSLPYLRSIAL
                     GGGSVIKPLDGGKKGVQLGPESMGSYPGPVCYDLGGDQPTLTDAFVTAGLINPDYFLG
                     GTKPIHRDQAREAIEKSVAQPLQLSAEEACKAVIERSFEMVAEVIGAAQQEIGRDLSK
                     HTLFAYGGNGGLFACGVADKAGLKSIQMFSLGPVFSAFGSSVSDISHVYERALPEPSI
                     SEKAMAGMRKMIDEMKAEGAQDLLGEGISPENVSYGIEFEMSGKGAPPAPVACSEASL
                     KSPKDLEAALRTAANAPSGPLALDLIRLRVRKQMPKPHLKEQRLEPADSSHARAGKRD
                     VFWGSRSGEAQIYRWESLRPGNRVDGCAVVEGVNTTYFVPDGWTLTIDKFGDGTLNRE
                     "
     gene            complement(5922..9599)
                     /locus_tag="VN788_07820"
     CDS             complement(5922..9599)
                     /locus_tag="VN788_07820"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU20473.1"
                     /translation="MRGRSALLVFLAIAFFFGCANSVFAQGVATAQLSGTVTDPSGGA
                     VSGATVEVRNVATNTTSTAGTNANGFYAFASLAPGTYELKTSFSGFSNYTQTGIVLTV
                     GETATVNVALQLASQGERVVVTTEAQTIEPTKTEISQVVGTTQIQSLPINGRLFTDFA
                     LLTPGVATSRTSLGTSFTEYETTQISFGGMRSFSNEITVDGADFVNAASGVQRSTPPQ
                     ESVQEFRVVNNSFGAESGRALGGIVNIVTKSGTNELHGSLYEYFQNDVLNSRNLLQKP
                     EPGLQSTLLPKAYRHNQFGATLGGPIVKDKTFFFMNYEGKRHAESPLYPPDLVNNLDL
                     IDEAKSLMNLAPEGCFAGLAACQALGISNAGFLNSFVKTTDDDFGFARIDHQFNSNNR
                     LALRYNVEDSRNSGELVGQTLDGGGIGTPSGGRDLFIRDQSVVGTLDSVLRPNLVNTV
                     LMQYARRHYNFPGMTGQPDFSILNDLEIGHNFGTNDRLYESRFEFGDSVSWVKGNHVA
                     KFGFDGNKIWSLINFPGFMPTRMLVPTGASAASCLANFGEFYNTNFGAGAPTSLALDN
                     AAAGCPVPGDDGVVFTYAGISLPTDPNACSTAPCAPTVNSASPLDTTTWANAFPPALF
                     NGYSREIDHSYWGLYAQDQWRITPKLTINYGLRWDYESGLQSYVDRDFNEFQPRVGLA
                     WSPDTKTVVRAGFGIFFDRQNLTFFFVPNTQKVVAGYQCGNHPPSSIAAACAAGNTLP
                     QEFPNIQSNLGQAKQGYQIFGFPAAAGAADFAASIIASGGYTASPEHQTDVISLAGTC
                     ATTFACGIGEGGMDHNSRTPYAEQASLEVDHQFGGGLAVNLGYLFVGAHKLVRGNNIN
                     VPCPVGTTETAQATTPTDPNLWPLAPAFLGFPDWVPGLVNANGTFAKCGSGTPTLGTG
                     ALAGLGPWFQGAGPASGLQTMSSGLEDYNNDVSNAVYHGGTLTVMERIRNFNLTANYT
                     YSHTIDNGNFTTFINLPVNQFDLKDERANSNQDARHRLVTNFTATGPEDTFVRHFTLS
                     SIVTLQSGRPFTIFYGANTLNDVAGGATDRVGGAPVTGHCSSASNCATMIGRNTYVGD
                     PLYDWDLHLGRYFQIKEGLRLDLSVDAFNLLNRSNVDEVTSVYGSPVFCGGVIPRRYR
                     DARSRAIQSGAASMACPVGQTIVPGGSLANTPINDLPPGLGPGFVPTTLFIPSNPNRT
                     FGLPRTMLNPREFQFAAKFSF"
     gene            complement(9898..>10177)
                     /locus_tag="VN788_07825"
     CDS             complement(9898..>10177)
                     /locus_tag="VN788_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU20474.1"
                     /translation="CTKRYLHMIFHSEDVSISDYILKLRLERCREDLLNPACAHKSIT
                     DIAYSWGFNNSNHFSRCFKRAFGVAPRRTRSEFAPWPTASPEKHLKLS"
BASE COUNT         2111 a         3007 c         2882 g         2177 t
ORIGIN      
        1 gccacctgcc gccacagcgc cgcattcgaa aaaatcatct tggcgaaact gttttgcgtc
       61 gcgcaccgcg cctgcatgcc gatgtacacg cagcagctcg tcgcggccat cttgtccgga
      121 tacacgatgc tgccttccgg aaaaacgaaa tccagcggcg cgaatgtcga gctgcagatc
      181 ggcagatcgt ggaacatgta ctcgtacatg aaattggcga tgtgccccac taccgcttgc
      241 ggatgcacgt tgtacgacga cggattttcc ggcgacgtcc cgtgaaaatc aaaaatgatt
      301 cccccgtctt tcttggtgag cgtcaaataa cacgagcgca ccagcgccgg cgtccagccg
      361 attgcatcgt tgaaaaccac cgtgcggaat tttccgtccg gcaattcctt cacaatcttt
      421 cgcgccccgg cctccgcggt gtcggacatc ttgcgcagca atccgattac aaaatccgcg
      481 ccgcgtttat ccgccagctc gagcaagcgt acccgaacgc gatccgccgt agtgcaccgc
      541 gcccgcaaat ccgaaatgaa catcgccggc gcccgcatcc catacgccga atacatttcc
      601 aaaaaatcct gccggatctc gaaattctct ccgattttga tgggaggaag attcaaaccc
      661 tcctcgaaac gcgatttcgc gctcaccggc atgccgcccg gctccgtcgc gcccgtttcg
      721 gtggtgtgca acgccgccga ggaccacgca atcagcttcc cctgatgaaa aatgggcatc
      781 agagccactt ggtcgggatt gtgaatcccg ccgtacagcg cgtcattggt gtaccagacg
      841 tcgccgtccc gaatgcccgg attcgtttcg taatactttc ggatgaactt gatgatgatc
      901 ggctggatga tcgcgtgcaa atacgtcccg catgccgcgt tgatcaaatc tccgtttgcc
      961 gtgaagatcg ccaccatcga gtcgccggac accccgaagt agctgcgcga tgcgcggacg
     1021 aacacctcca tcgcttcatc caaaatatcg ttggtgatct gcacgccgat ttcgtaatcg
     1081 ccctggcgca ctttcgcggc gcactcgatt tcgttcggtg tggcgggcgc gggcttcagc
     1141 cattgcagtt tttccgccag tgtgggaata ggcatggtgt cctcgatcct ttaactgccg
     1201 gccgcttcca gaatgccgag cccgcgctca tcaattgaaa atcgcattcc cggcggcact
     1261 accatcgtgg tgtactgccc ttcgacaatc gccggcccgt ccactacatt cccaggacgc
     1321 aaagattcgt aggtaaacgt cggtgttggg cgaaacgttt tttcttccgg ccaatacgcc
     1381 gggcgttcgc ctttgcgcgc cccggaaggg tcttttcctt caagcggaag cttcggcaag
     1441 tgaactttcg gcgtcgttac gatcgctttc agaataaagc tctcgataaa aatgccgcct
     1501 tcgggattca ccacaaacgg gctgaacgcc tcgctgaact ctttattgaa cgcgtcgcac
     1561 acggcgcgaa tgtcctcgtc cgagtgcagc gaaagccgcg gcgaaagcgc gcgtttcacg
     1621 tggaactgcc ctccgtaaag catgtccagc tccagaacgt acgcggccgt tttgggatcg
     1681 acgccgtcgg cggacaagtc cttgcgcgct tgttccaata gcgcctcgac ggttgcgttg
     1741 taggccgcgt aatcgtgagt gagctgctgg tccccgggcg tcatcagcgt aatcttcttc
     1801 gacgcctcgt aaacgtgcat gatatccatc gtcgacgagc catatgcgca aaaaaccgga
     1861 gaaaacggga aaaggactgc tttctggatg tctcccttga acccttcggc atgcgtggcg
     1921 cctgccccgc cgcccacgaa cagaatgaaa tcctcggggc tgtatccgcg cagatgcacc
     1981 tctttcatga tcgcggacga catgtttcca ttcacgattt ttcggatcag cgccgcgcct
     2041 tcctccacgc tgattcctag cggcttcgcg actttgtccc gaatcgcctg aaccgcgcgg
     2101 ttcttgtcga tccgcatttt tccgccgaag taataatccg ggttgatgta cccgagcacc
     2161 acatccgcat ccgtgaccgt cggctcaact ccgcccaggt tgaagcatgc cggccccggc
     2221 atcgatcccg cgctgcgcgg tcccacctgc acacgatttt gaagcaaccg gtttacggac
     2281 gcgatcgagc cgccgcccgc tccaatcgaa agcgtcttga tcatcgtaat gcccaccatc
     2341 cagcggtcga tgatcggcct gaaatcgtag ctgcggacgc tgtccttcac caccaatcca
     2401 acgtcgaaac tcgtcccgcc catgtccgtc atcaccacgt tttggtagcc cagggacttg
     2461 gccacgtgat gcgcgcccat cagcccggat accggcccgc tattgaacgt gcggctggca
     2521 gaagtgcgaa aaacttcggc aatcccgcct gaactctgaa tcatcaacag cggctttttg
     2581 tagccgcgct cgcgcagctt gtcccacatg gccgaaagct cgatctgcat cgagcgctgg
     2641 agatacgcgt cgagaatggc cgccagtgtg cgctcgtact cacccagttt tccaacaacc
     2701 tgtcctgcga gtaccacggg catgtagccg atgtgaaatt ccttgtactc gtcgcggatg
     2761 atttctttca ctcgccgctc gtggatcgga ttcagaaaac cccacagcag cgaaaccacg
     2821 aatcctcgcg ccccgcgcga caccaggtat cggactttct ctcgaacatt gttttcatcc
     2881 agcggccgga tcaccgtgcc gcgcgaatcc actcgttcct tcacgcccac aatcaaatcg
     2941 cgcggtatta acggatgcgg tttgctctga atggcaagat tgcgccgctc ctgaacgcgc
     3001 gtgccatcga tccactgcgc accccgcccg atcaggacca catcctcatg cccttcggtg
     3061 gtaatcaatg ccagccgcgg cccttttcgc tcgatcagcc ggttcatggc gatcgtcgtc
     3121 gaataccgaa tcatgtcgat cgccggcagc agttggtcga tttgcatgcc gagtgttccc
     3181 gcgccttcct caatcacctt cgagaagcac acggagaggt catacggggt cgtgggaact
     3241 tttttgatca gtgccttccc gtcgaaattg aggactaagt ctgtaaaagt cccgcccacg
     3301 tcgatatcga tcgaattcat tccgccgcct cgagccgctt ctcgcggatt gtgaactcgc
     3361 ctttgcgcac ccgttctttc aggctgtcca ggtcgatttc gatgtcgtgg gtgatgggat
     3421 gccccggcgg aagatattcg gtctcgattt gcgtcccgca gccggggcag tagcactcta
     3481 caatgcgcac ccagttcgga tcgggagcga aattaaacga ggccgaaacc acttgcggat
     3541 gaatttcgcg cggatcgcgg tcatagagca agcagccctt tttgtaattc tcccgcgccg
     3601 gcccgaggac gtgcccgcac cggttgcaca tccagcgttc cgtctccaga tcgagatcca
     3661 gatattcggt gatgcgaatc tggtttttcc cttcggccat gatcgtaatc ctctattccc
     3721 gattcagcgt cccgtcgcca aatttgtcaa tggtcagggt ccatccatcc gggacaaaat
     3781 aggtggtgtt caccccttcc accaccgcgc agccgtccac ccgattgccc ggccgcagcg
     3841 attcccagcg gtagatctgc gcttctccgc tccggctgcc ccaaaagacg tcgcgtttgc
     3901 cggcgcgtgc gtgcgacgaa tccgctggct ccaaacgctg ttcctttaga tgcggtttcg
     3961 gcatttgttt tcttactcgc aggcgaatca ggtcgagtgc aagcgggccc gatggcgcat
     4021 tggccgcggt tcgcagcgcc gcctctagat cttttgggga ttttaacgat gcttccgagc
     4081 aagcaaccgg tgccggaggc gcgcccttac cggacatttc gaattcgatt ccatagctca
     4141 cattttcagg agaaattcct tcgccgagca aatcctgcgc gccctccgct ttcatctcgt
     4201 caatcatttt tcgcatccca gccatcgcct tttccgaaat cgatggttcc ggtagcgcgc
     4261 gctcgtacac gtgcgaaata tccgaaacgg aagaaccgaa cgcgctgaaa accggcccca
     4321 gagagaacat ttgaatgctt ttcagtccgg ctttgtccgc cactccgcat gcgaagagcc
     4381 cgccatttcc cccgtacgcg aaaagcgtgt gcttggataa atcccggccg atttcttgct
     4441 gcgccgcgcc aattacttcc gccaccattt cgaacgatct ctcgatcacc gctttgcatg
     4501 cctcttccgc ggacagttgc agcggctgcg ccaccgattt ttcgatggcc tcgcgcgcct
     4561 gatcgcgatg aatcggtttc gtaccgccga ggaaataatc tggattaatc agtcccgcgg
     4621 taacgaaggc gtcggtaagc gttggctgat cgccgccgag atcgtagcaa acgggcccgg
     4681 gatatgaccc catgctttcc gggccgagct gcacgccttt cttcccgcca tccaacggct
     4741 taatcacgct gccgccgccc aaggcgatgg agcgcaagta gggaagcgac aactcgaccg
     4801 ggatgccaaa caggtccgat ggcttgcggt acatcggctc gccgtccgcg agaacgctga
     4861 ttttcgccgt cgtgccgccc acgtccatcg cgatcacgtc tttcaatccg cgcgctttcg
     4921 cttggttcgc gctgcccaaa attcccagca ccggccccga ttccagcgtg tcaagcgctc
     4981 gcgtcttcgc gattccagcc actccgccgt tgatgtggct caccaaaaaa gttcccgcgt
     5041 aatgcgatgt ttcgcgcaaa tcgtcttccg ccttgaacag cgtggctgcc agcgcgccgt
     5101 gtgtgtacgc attgattact gcacaaaacg cgcgcgtccg gtcgtccgaa ctcttggaaa
     5161 tatcgcttcc cgcgagaacc ggaaccgaac cgagaaaatg atcgggatat tgctgatcga
     5221 tagtccgttt ccaggaaatt tcccgctcgg gatggcggtg cgaatcctcg aggctgatgc
     5281 agatcctgcg tacgccgctt tccagcagga gccgcgccgc attcatgatc gcggtttcat
     5341 tggccgaccc gtccaggcca atcacatccc ggctggagag aagccggttc agaaccgcgc
     5401 tttccgctcc gttgccgtag agatttttct gtcgcccctc ggaaaccagc aacccgatgc
     5461 gcggcccgcg cagctcagcc agtacgttgg atgtgatggt cgtggaccag cgaatcagat
     5521 cgacattttc gagaaaggaa gctgtgtccg cgaagccgag tttctccgcg ccctgtgcga
     5581 ggcagtcgaa caggcagacg gtcaaatcgt gcggcgtggt gtccactttg actgacacca
     5641 ccttgtcgcc gctgctgaag aggccatcag tgagcgttcc gcctacgtcg atgtccacag
     5701 tccacacgag aaattgtccg ttccagtcgc gaggcgtgat caggctgcca tagcgaagca
     5761 attcgaaccg ctgagagtac tacacggcgc agccgcgtaa caagcagggg ttagagggca
     5821 aaggccgggc agaacccggc cttttttgca ccttaaatgt ttcgagttag aagaaactga
     5881 agctgcaaac tagaactgtt ttccgcgcaa attgtttcta gctagaacga aaacttagcc
     5941 gcgaactgga actctcgcgg gttaagcatc gtccgtggca gtccaaaagt tcgattgggg
     6001 ttactcggga taaacaaagt tgtcggcacg aaaccggggc caaggcccgg cggaagatca
     6061 tttatcgggg tattagcaag ggatccgccg ggcacgatgg tttggccgac agggcaagcc
     6121 atagacgctg cgccggactg gatggcccgg ctcctggcgt cgcggtaccg cctgggaatg
     6181 acgcctccgc aaaaaacggg cgagccgtac acggacgtaa cctcatccac gttcgaccgg
     6241 ttgaggaggt tgaatgcgtc cacgctgagg tcgagcctca gcccttcctt gatctggaag
     6301 tatcgcccaa ggtgaagatc ccagtcataa agcggatcgc cgacgtaggt gttcctcccg
     6361 atcatagttg cgcaattcga cgcggaactg cagtggccgg tcaccggtgc gccgccgacg
     6421 cggtccgttg ctccgccagc gacgtcgttg agagtattcg caccgtagaa aatcgtgaag
     6481 ggtcgccccg actgcaacgt aacgatgctg ctgagcgtga agtggcggac gaaggtgtcc
     6541 tcgggccccg tggccgtgaa gttcgttacc aagcggtggc gagcgtcctg gttcgagttg
     6601 gcgcgctcgt ctttcagatc gaattgattc acgggcaagt tgatgaacgt ggtgaagttt
     6661 ccgttgtcga tcgtgtggga atacgtgtag ttggcggtta ggttgaaatt ccttattctc
     6721 tccataaccg tcaaagttcc gccgtgataa accgcattgg acacgtcgtt attgtaatcc
     6781 tcaagaccgg aactcatggt ttgcagaccg ctggcagggc cggctccctg gaaccacgga
     6841 cccaggccgg cgagtgcgcc cgttccgagg gttggcgtgc cggagccgca tttggcaaaa
     6901 gtgccatttg cgtttactaa ccccggtacc caatctggaa agccaagaaa cgcaggcgct
     6961 agcggccaaa ggttggggtc ggtcggagtc gttgcctgcg cggtttccgt ggtacccacc
     7021 gggcacggga cgttgatgtt atttccgcga accagcttgt gtgctccgac gaagaggtag
     7081 ccaagattca cggctaagcc gccgccgaac tgatggtcaa cttcgagact agcctgctcg
     7141 gcatagggcg ttctggagtt gtggtccatg ccaccctcgc cgatgccgca cgcgaaagtg
     7201 gtggcgcagg tgccggccaa tgaaatgaca tctgtctgat gttccggact cgccgtgtac
     7261 ccgccgctag cgataatgct agccgcgaag tctgccgcac ccgctgcggc cgggaatccg
     7321 aagatctggt agccctgttt agcctgtccc aagttggatt ggatgtttgg aaactcttgg
     7381 ggcagagtgt tgcccgccgc gcaagccgca gcgatggacg atggcggatg gttaccgcat
     7441 tgatagcccg cgacaacctt ctgggtattc gggacaaaga agaaggtaag gttctggcgg
     7501 tcaaagaata tgccaaagcc cgctcggacc accgtcttgg tgtccggaga ccaggccaag
     7561 ccaactcgcg gctggaactc gttgaagtct cggtcgacgt atgactgcaa gccggactcg
     7621 taatcccacc gcaagccgta gttgatggtt agcttggggg taatccgcca ctgatcctgg
     7681 gcgtagaggc cccaatagga gtgatcgatc tcgcggctgt agccgttgaa gagcgccggc
     7741 ggaaacgcgt tcgcccaagt ggttgtgtcc agcggactcg ctgaatttac cgtcggagcg
     7801 caaggagcag tggagcaagc gttgggatcc gtggggagcg agatgccagc gtatgtgaat
     7861 acgacgccat catcgcccgg gaccgggcat cccgcagcgg cgttatcgag ggcgagagag
     7921 gtcggcgcgc ccgcaccgaa attcgtgtta tagaactcgc cgaaattcgc caaacaagat
     7981 gcggccgagg ccccagttgg caccagcatc ctggtgggca tgaagccggg gaaattgatg
     8041 agcgaccaaa tcttgttgcc gtcaaacccg aatttggcga cgtggttgcc cttgacccag
     8101 gaaacagagt cgccgaattc aaacctcgac tcgtagagcc tatcgtttgt tccaaagttg
     8161 tggccgattt cgagatcgtt gaggatgctg aaatccggtt gaccggtcat gcccggaaaa
     8221 ttgtagtgac gacgggcata ttgcatgagc acggtgttca cgaggttagg cctgagcacc
     8281 gaatccaggg tgcctaccac cgattgatcg cgaataaata ggtcgcggcc tccacttggg
     8341 gttcctatgc cgccgccatc gagcgtctgg ccgaccaatt cgccggagtt tcgcgaatcc
     8401 tcgacgttgt agcgaagtgc cagccgattg ttggagttaa attgatggtc gatcctcgca
     8461 aatccaaagt cgtcgtcagt ggtcttaacg aacgagttga ggaatccggc gttggagatg
     8521 cccaatgcct ggcaagcggc aaggcccgca aagcaacctt caggagccag attcatcaat
     8581 gacttggcct cgtcgatcag gtccaagttg ttgacaagat ccggcgggta cagaggagat
     8641 tccgcgtgcc gtttgccttc atagttcatg aagaaaaaag tcttgtcctt gacgatcgga
     8701 cctcccagag tcgcgccgaa ttgattgtgc cggtacgcct tgggcaggag cgtcgattgc
     8761 aagcccggtt cgggtttctg gagaaggttg cgggagttga ggacgtcgtt ctggaagtat
     8821 tcgtataggg aaccgtgaag ctcgttcgtt ccgctcttag tcacgatatt gacaatacca
     8881 ccgagtgccc ggccagattc ggcaccgaag ctgttattga ctacacggaa ttcctgaacg
     8941 gactcctggg gaggggtgga acgctgcacg ccgctggccg cattgacgaa atccgcgccg
     9001 tccaccgtga tttcgttgct gaaggagcgc atgcccccga aggaaatttg ggttgtctcg
     9061 tactcggtaa acgaggtgcc gaggctggtg cggctggtgg caacaccggg agtgagcagc
     9121 gcgaaatcgg tgaagaggcg cccgttgatg ggcagggact gaatctgtgt ggtcccgacg
     9181 acctggctga tctcggtctt ggtcggctca atggtctgcg cctcggtggt gacgacaacg
     9241 cgttcgcctt gcgaagcgag ttggagcgcg acgttaaccg tggcggtttc gccgacggtg
     9301 agaacgatgc cggtttgcgt gtaattcgag aagccgctga aggaggtttt caactcatag
     9361 gtccccgggg ccaggctggc gaaggcgtag aagccattcg cattcgtacc ggccgtggag
     9421 gttgtgttgg tggcgacgtt tcgcacctca accgttgcgc cagatacggc gccgccgctc
     9481 ggatcggtga cggtgccact caattgagcc gtggccacgc cctgcgcgaa gacagaattg
     9541 gcgcacccga agaaaaacgc gattgcaagg aaaaccaaca gagccgaacg tcccctcata
     9601 agccacccct cctcaaaagt gaagcgtgaa aaattagtag aggcccaccc attcaggcca
     9661 tctcaaaccc tttggattgt taaatgtgta gcgcccgtga cgggctttgt caagcgtttc
     9721 attggaacta tgtgcccgaa taggctcccg gactcggctc tcagcgagcc ccaagcaggc
     9781 cctcttcctg ctcacgaatt tgcgaaatat tagcgaagag cattcgggcg gtctatcccc
     9841 agagggaaaa acgatccatc tgagagggaa gcgcccttgc gccacttccg actggagtca
     9901 cgacaacttg aggtgctttt ccgggctagc cgttggccac ggggcgaact cggaccgggt
     9961 gcggcgcggg gccacgccga aagcgcgctt gaaacagcga ctaaagtgat tggaattgtt
    10021 gaagccccaa gaataggcga tgtccgtaat ggatttatgg gcgcaggcgg ggttaaggag
    10081 atcctcgcgg caacgttcga gcctcaactt tagaatgtag tcggaaatgg aaacatcttc
    10141 agagtgaaaa atcatgtgga gatatcgctt ggtgcag
//
LOCUS       DATNDT010000246        16796 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1013707, whole genome shotgun sequence.
ACCESSION   DATNDT010000246 DATNDT010000000
VERSION     DATNDT010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16796
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013707"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..973
                     /gene="rpoD"
                     /locus_tag="VN788_07830"
     CDS             38..973
                     /gene="rpoD"
                     /locus_tag="VN788_07830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXU20475.1"
                     /translation="MERLLDVEREARRLERRVGAALGEVAIEVRKELRARRAELEQIE
                     ESSEVGLNSLKRSLALIERGEAQALEAKKELTEANLRLVVSIAKKYTHHGLHFLDLIQ
                     EGNLGLMKGADKFDWRRGYKFSTYATWWIRQAITRAIADQARTIRVPVHMIEVINKLA
                     KCTRQLVQELGREPTSEELAKRLDMTIEKVRQTRKIAQTPISFETPIGEEEDSHLGDF
                     IEDKAVVSPSEAAMNLNLKEKMASVLKKLSPREERIIRMRFGFEDGNQRTLEEVGQVF
                     AVTRERIRQIEAKALRKLRHPSHSSPIRTFLEDSW"
     gene            1020..1229
                     /locus_tag="VN788_07835"
     CDS             1020..1229
                     /locus_tag="VN788_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20476.1"
                     /translation="MSLTFQKRQKEFKRQEKQRAKAEKREQKKLAKRAVEAQGTAITL
                     LPDSGQQLQSEDKERVPPEQYEGES"
     gene            complement(1416..2357)
                     /locus_tag="VN788_07840"
     CDS             complement(1416..2357)
                     /locus_tag="VN788_07840"
                     /inference="COORDINATES: protein motif:HMM:NF016302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HXU20477.1"
                     /translation="MCTSPEVVTTIETENNRRVHHSHSNGRSKSIGKLISALAAAQRE
                     YKPIKRATENCSYTTETRKAMYAELQDVIAATQPALSAHGLAIIQRVSSDLKRCVSII
                     ETILAHESGQWVANVLELPAIGWDQRWNDEAKRKEWYRRFDSQTLGIAFTYGRRYSWL
                     AIAGVSSEPDDDANAISAAAQRGSAEAAQAVANEKIATLKQKVGNGLKTASDLEKQLK
                     AGLDLVRQKKACYTCGAEDHCASECPAPISKVAPDPTRVLFVTQDFVSNTYQITGSAE
                     LMKETGWKNFAGLSGDEAADFNYLCEKMGITLKKLSR"
     gene            complement(3276..3692)
                     /locus_tag="VN788_07845"
     CDS             complement(3276..3692)
                     /locus_tag="VN788_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004175208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HXU20478.1"
                     /translation="MSDRVFFDTNVLIYILARNDPRSKRAEELLAAGGILSVQILNEF
                     VSVARRKLLMSWSEIAEALAAIRILCRSPLPLTVETHEAALKIAERHGYGIYDALVVA
                     TALDAGCETLYSEDLHDGHTIEGQVTIRNPFEHGSI"
     gene            complement(3682..3912)
                     /locus_tag="VN788_07850"
     CDS             complement(3682..3912)
                     /locus_tag="VN788_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXU20479.1"
                     /translation="MHVSKWGNSLAVRLPSAVVDALGLKEGDQIEIRIVDKRVFEVGR
                     DESRQRALARLRKLQRPLPAGFVFDREEAHVR"
     gene            complement(3986..6313)
                     /locus_tag="VN788_07855"
     CDS             complement(3986..6313)
                     /locus_tag="VN788_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HXU20480.1"
                     /translation="MSIPKDINDYMRAWASALGERILQAFPPLHGFDAAPSPLVEHLL
                     RRPFPAQTLAIMGVVKRWDEARGAAVVAECGTGKTLISLGALHVHSDRKPFTAIAMVP
                     PQLVDKWAREAFKTLPRVRVFFIDGLRTATGSAPHTGVNEVRLRRDRIVREGLRTTLT
                     QLRLRKTARTARQRWDSICGSPALFIVGRDRGKLSYFWKHAYRLARCGRYRGSVVNPD
                     SGCPVYLGEDDQRLLSADFKKAKLSEILGVRNGEQPAKLRRSLYSALWQADRNKTRRF
                     APIDFIGRHMTDFFDYAIADEVHELKGDTAQGNALGTLATCAQRTVVLTGTLLGGYAD
                     EVFNILFRIEPAKMLRQGFEYGEAGVRAFTETYGLLEKVTVVDPEDNACSEARTSKRV
                     RRRPGASPLLFGRFLMSLGAFISLEDISEALPPYREELVSVEMDASLKEAYSNLEENV
                     KIALKEHRGNQSVMSVALNALLLYPDRPFQLGSLYGWEFNPETQRREQFLIAEPWDLS
                     EELVYAKERRLVEEVKAELACGRRCQIYAVYTQKRDVTRRLERILCDAAIRVAVLTTD
                     VPPESRETWYDRQLRNGVQAVICHPKLVQTGLDLIEFPTILFYETGYSIYVLRQASRR
                     SWRIGQRQPVKVKFLYYAETMQERCLRLMGKKLLVSLAMEGKFSSEGLQAINEEDDIM
                     MAMARELVTEKGIGEKADAIWGALQKKQEEVLGIRPSAAEGLAPEHAQNIELVVAETA
                     SMPCSQLIPLAAPLKAIRRRLPRRECVLDDQLTLF"
     gene            complement(6457..6795)
                     /locus_tag="VN788_07860"
     CDS             complement(6457..6795)
                     /locus_tag="VN788_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20481.1"
                     /translation="MAKTKPGTDREGASTILCGDDPVFVLYRLASRYGLPVVPEWANW
                     FMKELNRQKAIASLLGLGCTPVRVKGTKQTFLKWIRRGLREGLIRIPEANSSIRWQMP
                     ISLVGNTYLR"
     gene            complement(7248..7514)
                     /locus_tag="VN788_07865"
                     /pseudo
     CDS             complement(7248..7514)
                     /locus_tag="VN788_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009574197.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7894..8709)
                     /locus_tag="VN788_07870"
     CDS             complement(7894..8709)
                     /locus_tag="VN788_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA family kinase"
                     /protein_id="HXU20482.1"
                     /translation="MDTTCVFAVQHLRRMRGGAQSHLLQASDGAHYVTKFMNNPQHVR
                     VLANEMLATNLGLRLGLPMPRVEVINVSDRLIEDTDDLRVKLGRASIPCSCGRQLASL
                     YVGDTVETVICDYLPDAVMEDVVNIQDFARVLVLDKWTCNSDGRQAVFKLEASPSRRY
                     TAVFIDQGFCFNGGEWNFPDAPLRGAYARQRVYENVKAWESFEPALTRAEQMDLEELW
                     RYAAQVPQEWYEYDVSGLNRLVETLYRRRLRIRDLISDFRKSNRNPFPNWVER"
     gene            complement(8788..9903)
                     /locus_tag="VN788_07875"
     CDS             complement(8788..9903)
                     /locus_tag="VN788_07875"
                     /inference="COORDINATES: protein motif:HMM:NF039653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HXU20483.1"
                     /translation="MVCIALRDAIQLLYSAIARISTSEKGPEEVSMRIAARTKLGFYP
                     LPLSEAERIRRFLAFPDTHCSALDPCAGDGAAFAEIASAGRVSLYGVELEAGRAKQAR
                     ARGIDVIHANCFDVQCPVESFSLIYLNPPYDFEISEEKSQRMERLFLEHVYRWLKPLG
                     VLVLVVPAKRIGDCAVLLSRHFRDVRIYRLTEPESVRYHQTVVLGIRRTRQERDRLRE
                     SEIVQAQLWLRSLDRKLEELPLLAEPDYTYAIPVGAPARLVHRGLPLDEIEDLLPKSS
                     AYRQASAIIFAQQPEVGGRPLTPLHGGHVGVLCTAGMLNGIFGEGETRHIAHWQSVKL
                     VDKSEEEEDGKIIIREKERFSNELTLVFCSGEIATLK"
     gene            complement(10179..10556)
                     /locus_tag="VN788_07880"
     CDS             complement(10179..10556)
                     /locus_tag="VN788_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20484.1"
                     /translation="MAHPLKHAQSSAKRFGGKPEDYLAIHNWFDESKAFLADFRHRGL
                     RHHAEGIFLCERLFGISILNSEGKEIPVRYIGEQHVKEDLGRIPTAQDWLTRIKPERW
                     MCGQRLQAESEPATGCHPQHPHS"
     gene            complement(10558..10926)
                     /locus_tag="VN788_07885"
     CDS             complement(10558..10926)
                     /locus_tag="VN788_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20485.1"
                     /translation="MPEEQKPPLDKEGSPQQIATFKRTIIPKLVELGVSKLTINYSGS
                     GDEGAVDGLNVEPEGVVIPADLEKQISDLADEFLYAEHGTWEDGDGGTGTIVIDPARG
                     KIVNEHGWYFTDVDYETKEF"
     gene            11280..11696
                     /locus_tag="VN788_07890"
     CDS             11280..11696
                     /locus_tag="VN788_07890"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU20486.1"
                     /translation="MNNGRNPTGNGQASDPTLVACVVFETQDPEQLVELLRQALTGIS
                     DQARPRSPETASVSLARTPQDLWLKHGEAAEYLGVSKSTLYQYACQQKIECRKLAGRL
                     EYRRSTLDQFKDRQIRPAHRWFPRQGIIPPALGSGK"
     gene            11708..12787
                     /locus_tag="VN788_07895"
     CDS             11708..12787
                     /locus_tag="VN788_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXU20487.1"
                     /translation="MGLYRKRKSPYWWMSYMVDGEQCWESTKTRSRNAALRIWKKREA
                     EIALGKFKVGWPGQRITFEELCDEFEKSHFAGISENTITGYRIYLKHLKQFFGGLVLL
                     KLTTKLIEEYRDHRRQQPSVRYKGRTLKGASVNRELECLTCVMDLATRRQYIPANPAR
                     GVKHFNELRERPDKQMLTLNQENRILKAAPPHLRVGIILLVQTGGRTYSEGFRLRWNQ
                     VDWEHRLIRLGNDVKTPGSSEPLPLTDLAYRVLSEWKKELGSDSPFVFPSPRKPDRPI
                     RTVKTAWKATLRRAGVPYFPIYNLRHAFCTRLSWVAPDAVIQRAMRHTSPETKRRYQL
                     SMVEQVREAMEKANQRVYGDQGTIQ"
     gene            complement(12876..12950)
                     /locus_tag="VN788_07900"
     tRNA            complement(12876..12950)
                     /locus_tag="VN788_07900"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12916..12918),aa:Asn,seq:gtt)
     gene            complement(13056..13358)
                     /locus_tag="VN788_07905"
     CDS             complement(13056..13358)
                     /locus_tag="VN788_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20488.1"
                     /translation="MSTQANPATLTTVLDDLVRDLDFPQREAAFFYGLFLRGHSAEEL
                     RRDIEVPAAVLAKWDRECLRQPEQRSNFERIAEYRRHVLAIFDSLVGFDSKTQRLQ"
     gene            complement(13438..13707)
                     /locus_tag="VN788_07910"
     CDS             complement(13438..13707)
                     /locus_tag="VN788_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20489.1"
                     /translation="MWIRRDWDEFFNRIRRRGAVANPPRPVDRSARNRLVPAEGYSLA
                     ELDDAGLSLEQAEVLGLPVDALRVGSDQANVTALQMFLRASRSRY"
     gene            13946..15211
                     /gene="thiI"
                     /locus_tag="VN788_07915"
     CDS             13946..15211
                     /gene="thiI"
                     /locus_tag="VN788_07915"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HXU20490.1"
                     /translation="MQSKELPGTQPVIVLHYHELWLKGRNKRYFLSKLAANLRAALEG
                     IAVEKIERPNDRFIIRLAPGVALDEVLRRIERIAGIAYYAVAHPVERDLNAICEAAWQ
                     ETRDLSFNSFAVRAKRSDKSFPQGSSEIERSVGRFLLDSFQAADRSVRVQLNNPDVTC
                     RIEVTSGPALVYAKKIQGAGGMPANTAGKVMCLLSGGFDSAVAGYQMMKRGAHLSFIH
                     FYGTGAQPGESSIQVARKLVEQLVPWQFTAKLYRVPFEPIQREIVRYAPESSRVLLYR
                     RMMLRIAEILARRSRALAVVTGDSLAQVASQTLRNLVAVEAASRIPVFRPLLGADKLE
                     IIETAKKIGTYDISAEPFHDCCPIFLPRNPELFSTPAALESAESNLDVAKLVQQGVES
                     AMVETFQYRAGKVEITEKTAASKRKTAMA"
     gene            complement(15241..16452)
                     /gene="argJ"
                     /locus_tag="VN788_07920"
     CDS             complement(15241..16452)
                     /gene="argJ"
                     /locus_tag="VN788_07920"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:NF003802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HXU20491.1"
                     /translation="MDSLSETHLPRGFTFSAATCGLKKSGLDLGLLVSEVPSAAACLF
                     TTNRVKAAPVLVSQMHLRKSRGRMRGIIVNSGNANCATGTEGIAIAATTALKTSWELG
                     RYHPSQILVCSTGVIGVPVPAQKITKAIPGLVRTRRASADAFEQFTRAIMTTDTRPKW
                     ASASCAIAGKRVRLLGCAKGAGMIHPQMATMLAFIATDVAVSPNVLRRALREAAAKTF
                     HAITVDGDTSTNDTLAALANGASGAREIRNGSNDYRKFAAALERVCRSLALQIVQDGE
                     GAKRLVEIIVRGAASDRAAERIALTIANSPLVKTAISGADPNWGRILAAAGRAGVSFN
                     PERVEISMAGMCMCSKGSALPFNENVAHRRLLGKQIPISLDLHSGKGLARVWTCDLTE
                     DYVRINASYRS"
     gene            complement(16455..>16796)
                     /locus_tag="VN788_07925"
     CDS             complement(16455..>16796)
                     /locus_tag="VN788_07925"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXU20492.1"
                     /translation="ALESYGENLAEIRSLAVSPELRGSGLGGKLLQFALEEARERNIA
                     RVFAVTHAPDFFVRQGFVLSSRRALPEKIERDCNTCPKARRCRLTAVVATLAPERDSF
                     PILQAVAGSPA"
BASE COUNT         3696 a         4748 c         4621 g         3731 t
ORIGIN      
        1 ggagaaaaaa cgcctaatcg agacagtgcg ttcgactgtg gaacgcctgc tcgacgtcga
       61 acgcgaagca cgccgccttg agcgccgcgt gggcgctgcc ctaggcgagg tggcgatcga
      121 ggttcgcaag gaactacgcg cgcgccgcgc cgagctggag cagatcgaag aatcgagcga
      181 ggtggggctg aacagcctca aacgctccct tgcgctcatt gagcggggtg aagcgcaggc
      241 tctcgaggcc aagaaagaac tgaccgaagc caatcttcga ttggtggtct ccatagccaa
      301 gaaatacacg catcacggcc ttcactttct ggacttgatc caggaaggca atctcgggct
      361 catgaaaggc gccgacaagt ttgactggcg acgaggttac aagttctcca cctacgccac
      421 ctggtggatt cgccaggcca tcacgcgcgc catcgccgac caggcgcgca ccatccgtgt
      481 gcccgttcac atgatcgagg tcatcaataa gcttgccaag tgtactcgcc agctcgtgca
      541 ggagcttggc cgcgagccca cttcggagga attggccaag cggctggaca tgaccattga
      601 gaaagtccgc cagactcgca agatcgcgca gacacccata tccttcgaga cgccgatcgg
      661 tgaagaggaa gattcgcatc taggggattt catcgaagac aaggccgtag tctctccatc
      721 ggaggcagcc atgaacttga acctcaaaga gaagatggca tctgtactga aaaagctcag
      781 ccctcgggag gaacgtatca tccgcatgcg attcgggttt gaggacggca atcagcgcac
      841 cctcgaagaa gtcggccagg tgttcgccgt aacccgtgaa cgcattcgtc agattgaggc
      901 caaggcgctg cgcaaactgc gccacccgtc gcactccagc ccaatccgca cgttcctcga
      961 agactcgtgg tgaagcaggt ttcgttgatt cattagaatc ggacttctga ggaggaccga
     1021 tgagcctcac cttccaaaaa cgtcagaagg aattcaagcg tcaggaaaag caacgggcga
     1081 aagccgaaaa gcgtgagcag aagaaactcg ccaaacgcgc agtggaggcg caaggcactg
     1141 cgataaccct gttgccggac tccgggcaac agcttcaatc cgaggacaaa gagcgggtcc
     1201 cgcccgaaca atacgaaggc gaaagttaga acaccgttcc tgtgaaaagg ctggccagcc
     1261 cggtcttgct tttcgacaca tcgggcacgc ttcgtcaaac accatttgcc ctaaccaagt
     1321 accagttgcg ggatttcgcc gccgtatagc tacccccgtc cataccacta tattctcaga
     1381 gttacttccg cttttcagcg agggaggggc cgtagtcatc ggctgagttt cttcaaggtg
     1441 atgcccatct tttcgcacag ataattaaaa tcagccgctt cgtcgccgct taagcccgcg
     1501 aaattcttcc agcctgtctc cttcatcaac tccgctgaac cggtgatctg gtaggtgttg
     1561 ctcacgaagt cttgcgtgac gaataatacg cgggttggat cgggtgccac cttgctgatt
     1621 ggcgcggggc attcggaggc gcaatggtct tctgctccgc aggtgtagca agcttttttc
     1681 tgccggacca ggtcgagtcc ggctttaagc tgcttctcca ggtcgctcgc tgttttgagt
     1741 ccgttgccca ccttctgctt gagcgtagca atcttctcgt ttgctaccgc ctgggccgct
     1801 tcggcggagc ctcgctgtgc cgccgctgag attgcgttgg cgtcgtcgtc cggctccgaa
     1861 ctcactcccg ctatcgcgag ccacgagtaa cgccttccgt aggtgaaagc gattccgagc
     1921 gtctggctgt cgaacctccg ataccattct ttcctcttgg cttcgtcgtt ccagcgctgg
     1981 tcccaaccga tcgctggcag ttctagaaca ttcgccaccc actgacctga ttcatgcgcc
     2041 agaatggttt caatgatgga cacgcacctc tttaggtcgc tcgacacccg ctggatgatg
     2101 gcgaggccgt gcgccgagag cgccggctgt gtggccgcga tcacgtcttg aagctccgcg
     2161 tacatagctt tgcgggtttc tgtggtgtag gagcaattct cagtggcgcg tttaatgggc
     2221 ttgtattctc gttgcgcagc ggccaaagcg gatatgagct tgccgatgga tttgcttcgg
     2281 ccattggaat gtgagtggtg aacgcgccta ttattttcag tctcgatagt tgtaactact
     2341 tcgggtgaag tacacatctt cctttctcct tggctgcatg cgcctcgatt cgccgcttga
     2401 tacggcatca gtcaggcagc ggtccaggcg cgaatggatt gcgcttggga tgaccaggct
     2461 ccgcgattaa tccccggagg cgatgaccgt cacaattgga cagccatctg tccgacgcga
     2521 acacgcctgc gaactcttcg acgttttcaa ttcgttgaag agccgccacg gggcaggtaa
     2581 tggaagacct cgccctctga aatgcaatgc gagccaaatg ccaccctctg tgaacacttc
     2641 agtgtcgaat tgaaaagctg tatcgccagc gagggtgaac tttcgacgag cttggccgac
     2701 acatagccaa ttgccggttc tggaatcgtg agtccgattg cgcgaccaat tgagcaggag
     2761 agccactggt tggcgagcac gcgcaggatg gttgaggaaa tggtgatctc acgtggtgcg
     2821 cgtgcgaagt ggttccacgg atcgccgaac cgccacgcga cttgcgaact caaatcagcg
     2881 aagtgcagct ttggggttgt cttttatggg tttgggggag gagagatccg aggcttaatg
     2941 gccgagccgc ttacgtcgtg agcttttcga acgatggact cgtacctcac ggtcgccatg
     3001 ggacacgcgc atggcgcgac gcacgcgcgg atagcgcgcc gccatgtcaa ctgcgacaaa
     3061 tagaatcggt accaatgacg ttgtacccag tgtccacaac ggcgatttag ggccgtgccg
     3121 ttctcaaggt acgtcaatgc gagaccgttg tcgggacgaa aaactcacgt tgccactgca
     3181 gaatgggatg gcctaatctt acggatgaga cggctccggg aaactaaatc acgaggcttt
     3241 tgtggaaaca acaccaacaa aagatgtgct gactgctaga tagaaccgtg ttcaaatgga
     3301 ttgcggatcg tcacttggcc ttcgattgtg tggccatcgt gaagatcctc ggagtagagc
     3361 gtctcgcaac cggcgtcgag tgccgtggca acgacaagag catcgtaaat cccataccca
     3421 tgccgctccg caatctttag tgccgcttca tgcgtttcta cggtaagggg gagcggggac
     3481 cggcacaaaa ttcggattgc cgctagcgcc tcggcgatct cgctccaaga catcagaagt
     3541 ttgcggcgcg caacggagac aaattcgttc aaaatctgga cgctaagtat gccgcccgct
     3601 gcgagcagtt cctccgctct cttgctccgg ggatcgtttc ttgccaagat atagattaag
     3661 acgttagtat cgaagaagac cctatcggac atgggcttcc tctcggtcga aaacaaagcc
     3721 ggcaggcaaa gggcgttgca gtttacgcaa acgagcaaga gcacgctgtc tgctttcgtc
     3781 ccgcccgacc tcgaagaccc gcttgtcaac gattcgaatt tcgatctgat caccctcttt
     3841 caggccaagc gcatcaacca cggcggaggg caaccgaacc gcgaggctat taccccattt
     3901 tgagacgtgc atcctatcct ccttgatata catgtgtaat tgtatatcct accattcttt
     3961 tcaacaagca gtttcctatt caccctcaga acagcgtgag ttgatcatcg aggacgcatt
     4021 cacggcgcgg aagccgacga cgaattgcct tcaaaggagc cgcaagcggt atcaactgac
     4081 tgcatggcat ggatgctgtt tctgcgacga caagttcgat gttttgggcg tgttccggcg
     4141 caagcccttc cgctgctgac ggtctgatcc ccaacacttc ttcctgtttc ttctgcaatg
     4201 caccccaaat tgcgtccgct ttttctccga tccctttctc cgtgactagc tcccgcgcca
     4261 tagccatcat gatatcgtct tcttcgttga tcgcttgcaa cccctccgag gaaaacttgc
     4321 cttccatagc aagggacacc aacagtttct tgcccatgag tcgcaggcac ctttcttgca
     4381 tcgtctcggc gtagtagaga aacttcacct tcactggctg acgctggcca attctccagc
     4441 ttcttcggct cgcttgccgg agaacataga tcgagtagcc ggtttcatag aacaggattg
     4501 tcgggaactc aatcagatcg agtcccgtct gcaccagttt cggatgacag ataacggcct
     4561 gcacgccgtt gcgaagctga cggtcatacc aagtctcgcg cgattcgggc ggcacgtctg
     4621 tggttagcac tgcgacgcga atcgccgcat cgcagagaat ccgttccagt ctgcgcgtta
     4681 catctcgttt ttgcgtgtag acggcataga tctggcagcg acggccgcat gccagttccg
     4741 ctttcacttc ttccacgagg cgccgttcct tcgcatacac cagttcttcg ctcaagtccc
     4801 atggttctgc gatgaggaat tgttctcgcc gttgtgtctc cgggttgaat tcccagccat
     4861 acaggcttcc caactgaaat ggccggtccg ggtaaagcag caatgcattg agcgccacgc
     4921 tcatgacaga ttgattccca cgatgctctt tgagcgcgat cttcacgttc tcttcaaggt
     4981 tgctgtaggc ttctttcaat gaagcgtcca tctccacgct gaccagttct tctcggtaag
     5041 gcggcagcgc ctcagaaatg tcttcgagcg aaatgaacgc tccgagactc atcaggaaac
     5101 ggccgaagag cagcggtgac gcccctgggc ggcggcgaac gcgcttgctt gtgcgagcct
     5161 ccgagcaggc attgtcctcc ggatcgacta ctgtgacttt ttccagcaaa ccataggtct
     5221 ccgtgaacgc ccgcacccct gcttcgccat actcgaaacc ttggcgcagc attttggcgg
     5281 gctcgattcg gaacagaatg ttgaagactt catcagcgta ccctccgagc agtgttccgg
     5341 tcagcactac ggtgcgttgg gcgcatgtgg ccagcgtgcc gagcgcgttt ccttgagccg
     5401 tatcgccctt caactcgtgc acttcatcgg cgatggcata gtcaaagaaa tccgtcatgt
     5461 gtcggccaat aaaatcaatg ggagcaaacc gcctggtttt gttccggtcc gcctgccaca
     5521 aggcgctgta caaggagcgc cgtaacttcg ccggctgctc cccgttccga acccccaaaa
     5581 tttcactcag ttttgctttc ttgaaatcgg ccgacagaag cctctggtcg tcctcaccga
     5641 ggtagacagg gcaacctgaa tcgggattaa cgacgcttcc tcggtacctc ccgcaccgag
     5701 ccaaccgata cgcatgcttc cagaaataac ttagctttcc ccggtcacgt ccgacaatga
     5761 acagggccgg cgatccgcag atggagtccc accgttggcg agccgtgcga gccgtcttgc
     5821 gcagccgcaa ttgggtcaag gtggtgcgca gtccttcccg cacgatccgg tcgcggcgga
     5881 gtctgacttc gttcactcca gtgtgagggg cagaacctgt cgcagtgcgc agcccgtcaa
     5941 taaagaagac cctcactcga ggcagagtct tgaatgcttc tctcgcccat ttgtcgacca
     6001 actgaggcgg aaccatagcg atggccgtga agggtttgcg atcggaatgc acgtggagag
     6061 cgccgagtga gatcagcgtt ttgccagtac cgcattcagc gacgaccgct gctccgcgcg
     6121 cctcatccca gcgtttcact acgcccatga tcgccagagt ttgcgcagga aatggcctgc
     6181 gaaggaggtg ctcgacaaga ggtgaaggag cagcatcaaa cccatgcagg ggtggaaaag
     6241 cctgaagaat ccgttcgccg agcgcggagg cccacgcgcg catgtagtca ttgatgtcct
     6301 tcggtatcga catagtcttt ccttcacggt ttggtagccc agaaaaagtc tcaaaaacga
     6361 attgtgttca gatggcaggg gtgatcgtcg tctggatacc gcttgttgga atttccgcag
     6421 gcattgcaac agcgccggga tgcgaccggc gcacagtcaa cgtaagtagg tgttccccac
     6481 gaggctgatt ggcatctgcc agcgaatcga gctgttggct tcgggaattc ggatgagacc
     6541 ttccctgagg cctctgcgaa tccatttgag aaaagtctgt ttggttcctt tgacccttac
     6601 gggcgtacat cccagaccaa gcagcgaagc aatcgccttt tgccggttga gttccttcat
     6661 gaaccaattt gcccactcag gtaccaccgg cagaccatat cgagacgcca ggcgatagag
     6721 cacaaagacc gggtcgtcac cgcacagaat ggtgcttgca ccttcgcgat ctgtccccgg
     6781 tttggtcttt gccaattccg ctgagaccgc aaccaggtgg cgaacaggac gcctccgacc
     6841 tgggaccatt acactcccgc gaaagcattg agcttcacac ccgagagagg cagccatagg
     6901 tgcgccacct ggaaattgga cccaaaacag agatccctcc gatacagcgg cccccaaggc
     6961 ccctatttcg gcatcactgc cgaccatgga tagaagatga gcgcctagtg gcactctgtc
     7021 accccttggt tgttctagaa tagcccgatc aatgtagact cgaacacgag tggtcgtgtc
     7081 tttcgtgcgc ttcatgaact ccaacgatcc aaagcgctga tgtgcgtttt gcaattttca
     7141 cttcctccag actgccgttg gatggattcg ccggtacatt cgcaccagac atcgccgacg
     7201 agatgatttc cagcgaagcg gccccgagca gttcgtcacc tatcttgcga gggaggaaaa
     7261 cccaggtctt ccctgacgtg ttgttctccg atatgcccga ccggaacctg gtttccatcg
     7321 ctgtttgtga tcgccacacc aaaaatcctc tcactcagga aaattccctc tgcgtggtgg
     7381 cggagggcgc gatggcgaaa gtcgccgaaa aatgctttcg actcatcaaa ccactggtga
     7441 acggcgagat agtccccggc ttgttccccg aacttctttg cgctgctctg cgcgtgcttg
     7501 agtggatgcg ccatagttag acttcatagg tcgttgttga gtagtcttcg aagcgatcat
     7561 tgcgctcaac tgtgagtgtg cgtttacgcg cgtccaagac cacatcgccg aacccgccgg
     7621 cgtcgttttc gtaaccgtac ggctcagcgc ctcgaaatgt tcttgctgat gggaaacgtc
     7681 gtgtgtgacc tgctcgcatt cttcgtaagt gtagatgtcg gcgtcgtagc acttgacgtc
     7741 ttcggtccgt cgtcgcccga tccatcgaat tgcgcggcga caaaggccgg cggcggccag
     7801 acagtcacat tgtgcgagga tcgcggcttt ggctgcgggt tggtgttgtt tttagctgtt
     7861 cttcgaacat taaatggtct tccacgaagt ctcctacctt tctacccaat tcgggaatgg
     7921 gttgcggttg gacttacgaa agtcactgat caggtcgcgg atcctgaggc gccttcgata
     7981 taaggtctcg accagccgat tcagcccact gacgtcgtat tcgtaccatt cctgtgggac
     8041 ttgggctgcg taccgccaga gttcttcaag atccatttgc tcagcgcgtg tgagcgccgg
     8101 ctcgaacgac tcccatgcct tcacgttctc atacacgcgc tgtctcgcgt acgcgccacg
     8161 cagcggagcg tcggggaaat tccattcgcc gccgttgaag cagaagccct ggtctatgaa
     8221 taccgccgtg tatctgcggc ttggtgatgc ctcgagcttg aaaacagctt ggcgtccgtc
     8281 gctgttacac gtccatttgt caagaaccag gacgcgcgcg aaatcctgga tgttaacgac
     8341 atcctccatc acagcatcag gaagatagtc gcaaatcact gtttcaaccg tgtctcccac
     8401 gtagagggag gcaagctggc ggccacagct gcacggaata ctggctcgtc ccagcttgac
     8461 gcgcaggtcg tcggtatctt cgatcagccg gtctgacaca ttgatcactt cgacgcgggg
     8521 catcggcagt ccaagccgaa gtccaagatt tgttgccaac atttcatttg caagaactct
     8581 gacgtgttga gggttattca tgaacttggt gacatagtgg gcaccgtctg aggcttggag
     8641 aagatgtgat tgcgcacccc ctcgcattct gcgaaggtgc tggaccgcga acacacaagt
     8701 agtgtccatt ttgcaaatct ccggctttga attgggttgc gagggtattt cagcgatcaa
     8761 cggtaaaggg tgggtgcggt acgtgagtta tttcagggtg gcaatctccc ctgagcagaa
     8821 gaccaaggtc agctcattcg agaatcgctc cttttctcga atgattatct ttccgtcttc
     8881 ttcctcttcc gacttgtcta cgagtttgac cgattgccag tgtgcgatgt ggcgcgtttc
     8941 accttcgccg aaaatgccgt ttagcattcc ggctgtacag agcacaccga catggccgcc
     9001 atgcaacgga gtgagtgggc gaccgccaac ctctggctgc tgggcgaaaa ttatcgctga
     9061 agcctgacga tatgccgacg atttcggcag caagtcctca atttcgtcca agggcagccc
     9121 acggtggacc agcctcgccg gtgcaccaac aggtatagcg tatgtgtagt caggctctgc
     9181 caacagcggc aactcttcca gttttctatc gagggatctc agccagagct gcgcctggac
     9241 gatttcgctc tccctcaacc tgtccctttc ctgcctcgtg cggcgtattc caagtacaac
     9301 ggtctggtga tatcggacac tctccggttc cgtgaggcga tagatgcgga cgtcgcggaa
     9361 atgcctggaa agcaaaacag cacaatcccc gattcttttt gcaggaacga cgagcaccag
     9421 cacgcctaag ggcttcagcc atctgtagac atgctcaagg aaaagcctct ccatgcgctg
     9481 cgatttctct tcgctgatct caaagtcgta cggcgggttc aggtaaatga gtgagaagga
     9541 ttccaccgga cactgcacgt caaagcagtt cgcgtggatc acgtcgatgc ctcttgcgcg
     9601 cgcctgcttt gctcgtcccg cctccagttc aacgccgtaa agtgagaccc tcccggctga
     9661 ggcgatttcg gcgaatgctg caccgtctcc agcgcaaggg tcgagcgcag agcagtgcgt
     9721 gtcgggaaac gccaagaatc tccgaatgcg ctctgcctca gagagcggta aggggtagaa
     9781 cccgagcttg gtacgtgccg cgatcctcat gctgacctcc tcggggcctt tttccgacgt
     9841 cgaaattctc gctatggcgg aatagagaag ctgaatcgca tctctaagcg caatacagac
     9901 caaagaggac cccgttgatt acagacgatc ctcattaatt tggctggcag atcggctggt
     9961 gctacattcc gccacgaaat ggaagccgcg gtccaagtca aagcagcggc ctgccgagtg
    10021 tgtgcgagtt tctggcactt tgcagcacgg ccacgaggga agaaattgtc cagaaccgcg
    10081 gcaatattgc cgaaagagtt ggcttcctgg gacgcttcat ccatggccgg aatccgcaga
    10141 cttggctcaa agaaaaagga cgcacctgag cgaacctctc aactatgcgg atgctgtgga
    10201 tggcagccgg tagccggttc gctttccgct tgcagtcgct gtccgcacat ccagcgttct
    10261 ggcttgattc gcgtgagcca gtcttgagct gttgggattc gtcccagatc ctccttgacg
    10321 tgctgttctc caatgtatcg cacggggatt tccttgcctt cgctattgag gatcgaaata
    10381 ccaaatagtc gttcgcacaa gaaaatccct tcggcatgat gccgaagtcc acgatggcga
    10441 aaatcagcga ggaacgcctt cgattcatca aaccaattat gaatcgccag atagtcttcc
    10501 ggcttgccgc cgaacctctt ggcgctgctt tgtgcatgct tcaaaggatg tgccatgtca
    10561 gaattccttg gtctcataat ctacatccgt gaagtaccag ccgtgttcgt tgacaatctt
    10621 gcccctggcc ggatcaatga cgatcgtgcc tgtcccgcca tctccgtctt cccaagtgcc
    10681 atgttccgca tacaaaaatt cgtcggcgag gtcggagatt tgcttttcca ggtccgccgg
    10741 aatcacaacc ccttcgggtt cgacattcaa cccgtcgaca gcgccctcat cacctgaccc
    10801 gctgtaattg atggtgagct tgctcactcc tagctcaact agcttgggga taatggtccg
    10861 tttgaatgtg gcgatttgtt gcggactgcc ctctttatcg agaggcggct tttgttcctc
    10921 aggcattctc ctatcctttc gggaaataga ttaatagaac ataaaaatta acagtggtat
    10981 tacctttggc cttttgggtg gcggtttgaa aggcaaatgg cagcgaaaga tagcaagtta
    11041 gctataaaac ggcgaattgc cgaaaaaggc caaaacccag tgtcaggtcc gattggcgtg
    11101 tgccgagtct tccagaccta acggcataac tctcagtccg acaggggatt gggctcgcca
    11161 gttcagtcgc acccaacgga actttgttgg cgctcatgcg ggagcaaatt gacatcatgg
    11221 gtcgcgggcc taagattccg ctgtctatcc tgttagcagc cgagctgctc ccaagcacca
    11281 tgaacaacgg tcgaaaccca accgggaacg gacaagcgag cgatcccact cttgttgctt
    11341 gcgtcgtttt cgagacgcag gaccctgaac aactggttga gctattgcgc caggcgttga
    11401 caggaatctc tgaccaagct cggcctcggt cgcctgaaac ggcgagcgtt tccctcgcgc
    11461 gaactcctca ggatctctgg ctcaagcatg gagaagcggc cgaatatctg ggcgtctcca
    11521 aaagcaccct ctaccaatac gcgtgccaac agaagatcga gtgccgcaag ctggctggcc
    11581 gtctcgaata ccgccgttcc actctcgatc aattcaaaga ccgccaaatc cgtccagcac
    11641 atcgctggtt tcctcggcaa ggtataattc caccggcgct cggctccggc aaatgagagg
    11701 agcgccagtg ggactataca gaaaacgaaa gtcaccgtat tggtggatgt cgtacatggt
    11761 ggatggtgag cagtgctggg aatccactaa aacacgctct aggaatgcgg ccctgcgaat
    11821 ctggaagaaa cgcgaggccg agatcgccct aggcaaattc aaagtcggtt ggcccggtca
    11881 gcggatcacg ttcgaggaac tctgcgatga gtttgaaaaa tctcacttcg cgggaatctc
    11941 cgagaacacg atcacgggct atcgaattta cctaaagcac ttgaagcagt ttttcggagg
    12001 actcgtgctg ttgaagctaa ctacgaagct gatcgaagag tatcgagatc atcgtcggca
    12061 gcaaccctcc gttcgctata agggtcgcac cctaaagggc gcttccgtca atcgcgaact
    12121 tgaatgcctc acgtgcgtca tggaccttgc aacacgccgc cagtacatcc cagcaaatcc
    12181 ggctcgtgga gtgaagcact tcaacgaact ccgcgagcgg ccagacaaac aaatgctcac
    12241 cttgaaccaa gagaaccgca ttctgaaagc ggctcctccg catcttcgcg tgggcattat
    12301 tctgctcgtc caaacaggag gaaggactta cagcgaaggg tttcggttgc gttggaacca
    12361 agtggattgg gagcatcgtc tcattcgctt gggaaacgac gtgaagacgc cgggctcttc
    12421 cgaaccgctt cccctaactg atttggctta tcgggttttg agcgaatgga aaaaagaatt
    12481 ggggtccgat tctcctttcg tttttcccag tccgcgcaaa ccggatcggc caatccgcac
    12541 cgtgaagacc gcgtggaaag ccacgctaag gcgcgcgggc gtgccgtatt ttccgattta
    12601 caatttgcgg catgctttct gtacgcgcct gagctgggta gcgccagacg ctgtaatcca
    12661 acgcgccatg cgccacacca gccccgagac aaagcgtcgg tatcaactca gcatggtcga
    12721 gcaagttcga gaagcaatgg agaaagcaaa tcagcgagtg tatggagatc agggcacgat
    12781 tcagtgattc tacatgacac ttacatgaca cctgacgttt caaaagcaaa acttggaaat
    12841 caggttctgc aagttgctga gagagaagga gaagctggct ccccgggcta gattcgaact
    12901 agcaaccctt cggttaacag ccgaatgctc taccattgag ctaccgggga attgcgggaa
    12961 tgccctcatg cagccggacg aaatgagtgt agtcgagctt cttcatgccg tcaatgcaag
    13021 gaggactctg ggtgggcttt cggctttaga aagaattact ggaggcgttg cgttttggaa
    13081 tcgaagccga cgagagaatc gaaaatcgcc agcacgtgac ggcgatactc ggcgatgcgc
    13141 tcaaaattgg aacgctgttc cggctggcgc aggcattcgc gatcccattt tgcgagcacc
    13201 gcggcgggca cctcgatatc gcggcgaagc tcttcggcgg aatgtccgcg caaaaagagg
    13261 ccatagaaga aggccgcctc ccgctgggga aaatccaagt cgcggacaag gtcgtccagt
    13321 acggtggtta aggttgccgg atttgcctgt gtcgacatag tgcaaaaacg cagtttactg
    13381 aattcatcgc ccgcgtcaag agcagaagag aggtgttttg aagaataggg aaagggccta
    13441 gtagcggctg cggctggcgc gaagaaacat ctgcagggcg gtcacattgg cctggtcgct
    13501 gccgacgcgc agggcgtcaa cgggcaggcc gaggacttcg gcctgttcga ggctaagtcc
    13561 cgcatcgtca agctcagcca aactgtagcc ttcggcggga accaggcgat tgcgcgcgga
    13621 gcggtccacc ggacgcggcg gattcgcaac agctccgcgc ctgcgtattc ggttgaaaaa
    13681 ctcgtcccag tcccggcgga tccacattgg accctcctcg ctcttcgctt agctaggggt
    13741 tagatgcaca atttctttgt agcgtcgcag cggtgcaaaa gcatctgctg cggcgagaat
    13801 tgtaacccct cgtgtcaaga aaagtagcga ttcagcagca tttgtatcca tagcgttagg
    13861 aatcagatga acacattgga ttcggtatcc aaagcgggcg aagggagtca ccgttgttgg
    13921 agcgggcttg ctataatggc ggcaaatgca atcaaaagaa ttgcccggca cgcaaccagt
    13981 tatcgtcctt cattatcacg agctttggct gaaaggccga aacaagcgtt attttctttc
    14041 gaagctggct gcgaatttac gtgccgccct tgagggtatc gccgttgaga aaatcgaacg
    14101 gccgaacgat cgttttatca ttcggctcgc tccaggcgtc gccctcgatg aagttttgcg
    14161 acgcattgaa cgcatagcag gaatcgcata ctacgctgtg gcgcatcctg tggaacggga
    14221 cctgaacgcc atttgcgagg ccgcgtggca ggaaacgcga gatctttcat tcaattcgtt
    14281 tgcagtccgc gcgaagcgga gcgacaaatc ttttccgcag ggcagctcag aaatcgagcg
    14341 cagcgttgga cggtttctgc tcgattcgtt ccaagctgct gatcgtagcg tgcgcgtgca
    14401 gctcaacaat ccggacgtaa cctgccgcat cgaggtcact tccggtccgg cgctcgtgta
    14461 tgcgaaaaaa attcagggag caggcggcat gcctgcgaac accgcgggca aagtgatgtg
    14521 cctactttcc ggcggattcg attccgccgt cgctggctat cagatgatga agcgcggcgc
    14581 gcacctttct ttcattcatt tttacggaac cggcgcgcag cccggagagt cctccatcca
    14641 agtggcgcga aaacttgttg aacaacttgt tccatggcag ttcaccgcga agctctatcg
    14701 cgttcccttt gagccgattc aacgcgaaat cgtgcgttat gcgccggaaa gttcgcgtgt
    14761 gcttctttat cggcggatga tgctgcggat cgcggaaatt ctcgcgcggc ggagtcgcgc
    14821 tctggccgtc gtcaccggcg acagcctcgc gcaagtcgct tcgcaaacat tgcgcaatct
    14881 ggtcgcggtg gaagcggcct cgcgcattcc cgttttccgg ccacttctag gcgccgacaa
    14941 actggagatc atcgaaactg ccaagaaaat cggcacgtac gatatttccg ccgagccttt
    15001 tcacgattgc tgcccgattt ttttgccgcg caatcctgaa ctgttttcga cgcccgctgc
    15061 gctggagagc gctgaatcca atttggatgt cgcaaaactg gtgcaacagg gcgtagaaag
    15121 cgcgatggtc gaaacttttc agtatcgcgc cggaaaagtg gaaatcaccg agaaaactgc
    15181 ggcttcaaaa cgcaagacgg cgatggcgta gcgttgaggt ttttttgcgg gcgggccggt
    15241 tcaactgcgg tagctggcgt tgatgcgcac gtaatcttca gtcaaatcgc aagtccacac
    15301 gcgagccagt cccttgccgg agtgtaaatc cagactgata ggaatttgct tgccgagcag
    15361 acggcgatgc gcgacgtttt cattaaatgg gagcgccgat cctttgctgc acatgcacat
    15421 cccagccatt gaaatttcta cgcgctcggg attgaacgac acgccagcac gtcctgcggc
    15481 tgcgagaatt cgcccccagt tcggatcagc ccctgagatt gctgttttga ccagcggcga
    15541 atttgcgatg gtgagcgcga tgcgctctgc ggcccggtcg gaagcagcgc cgcgcacaat
    15601 tatttcgacg agccgctttg cgccttcgcc atcctgcaca atttgcaacg ccaacgaccg
    15661 gcacaccctt tcgagcgcag ccgcaaattt ccgataatcg ttgctcccgt tgcgtatttc
    15721 gcgcgcgccc gacgcgccat tggcgagagc cgccagcgtg tcgttcgtgg aagtatcgcc
    15781 gtcaacagta atagcatgaa acgttttcgc cgccgcttca cgaagcgcgc gccgaagtac
    15841 gtttggcgaa acggctacat ccgtcgcgat gaatgccagc atggtcgcca tttgcggatg
    15901 aatcatgccc gcgcctttgg cgcaacccag aagccgcacg cgtttgccgg cgatggcgca
    15961 gctcgcgctg gcccatttcg ggcgcgtgtc tgtggtcata atcgcgcgcg tgaattgttc
    16021 aaacgcatct gcgctcgcac gccgcgttcg tacgagccca ggaattgctt tggttatctt
    16081 ttgagcggga accggcactc cgatcactcc agtcgagcac acaagaatct gagaaggatg
    16141 ataccggcct agttcccaag acgttttgag agcggtcgtc gcagcaatcg cgatcccttc
    16201 cgtgccggtc gcgcaattcg catttccgga attgacgatg attccgcgca tcctgccgcg
    16261 agatttgcgg agatgcattt gcgagaccaa aacaggcgca gccttcacgc gattcgttgt
    16321 gaacaggcac gccgcggcgg agggcacttc actgacgagc aatcctaaat ctaggccgga
    16381 ttttttcaat ccgcacgtgg cggcagaaaa agtgaaacct cgcggtaagt gcgtctcgga
    16441 aagggaatcc atttttaagc cggagaaccg gcaaccgctt gcaaaatggg gaaggaatcg
    16501 cgttcgggcg cgagcgtggc gactactgcc gtcaaccggc accgccgcgc ctttggacac
    16561 gtgttgcagt cgcgctcgat tttctcgggc agagcgcggc gcgaggataa cacgaatcct
    16621 tgccgcacaa aaaaatcagg cgcatgcgtg acggcgaaaa ctcgcgcaat atttctctcg
    16681 cgtgcttctt ccaacgcaaa ttgcagcagc ttcccgccga ggccgctgcc gcgcagttcc
    16741 ggactaacgg caagcgaacg aatttctgca agattttcgc cgtagctttc gagcgc
//
LOCUS       DATNDT010000247         7309 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_70543, whole genome shotgun sequence.
ACCESSION   DATNDT010000247 DATNDT010000000
VERSION     DATNDT010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70543"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="VN788_07930"
     CDS             complement(<1..277)
                     /locus_tag="VN788_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20493.1"
                     /translation="MKIWSDRTKNGGLTAALLSYAFAALVLATPHAAHEIKWLHLSSR
                     NRDLPNPGGSTEQTGLLVANLDKSAAAGFVISYRVNGPALVWFRRVGN"
     gene            complement(924..1178)
                     /locus_tag="VN788_07935"
     CDS             complement(924..1178)
                     /locus_tag="VN788_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20494.1"
                     /translation="MKIFWVSAVLLIPVGPIHAEEPAPVKDGLWSIHTQFIEHPREKK
                     NDTIGRGRPPNKEPTAGSSPYFCREAFPALISSQPVPWMK"
     gene            1266..1718
                     /locus_tag="VN788_07940"
     CDS             1266..1718
                     /locus_tag="VN788_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006890774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20495.1"
                     /translation="MDGEYVILYAQTEKEFMEYLIGVGLALVVCAFATLAGFDRDRVF
                     YPTLLAVIATYYILFAVMGNSTLALAIESAVAGTFLVLAVAGFKRSLWLVVAALAGHG
                     VFDFFHQLFIQNPGVPMWWPGFCLSFDILAGSFLAMLLMRRSGFALAT"
     gene            2546..3130
                     /locus_tag="VN788_07945"
     CDS             2546..3130
                     /locus_tag="VN788_07945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HXU20496.1"
                     /translation="MSEEKITADRLAAYSDAVFAVIVTIMVLELKAPDQPGFPDLWPL
                     WPTGISYVVSYLFIAIIWINHHYLWRFVGPPTLGLIWINFVHLFMVSLLPFATAWIAR
                     TRLASSPMVLYAGLFVCIDIAYNVFEHRVLAGADVTQVSARMRRMARRRSLAVLASFT
                     AAMLVAFVAPRVGFGLICAALVLHLKPDISGTRP"
     gene            complement(3170..3604)
                     /locus_tag="VN788_07950"
     CDS             complement(3170..3604)
                     /locus_tag="VN788_07950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019833376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXU20497.1"
                     /translation="MQRSDLARWAVLPMRLLVGYGFIEHGFAKLSRGADAFAGILQQM
                     GVPLPHVATWLTIGTELVGGLALLLGAFVVWASIPTALVLLVAMITVHLPYGFSSVKL
                     LSVSASGARFGPVGYELDLLYLAALLILALQGPGPLSIDGLR"
     gene            complement(3741..5492)
                     /locus_tag="VN788_07955"
     CDS             complement(3741..5492)
                     /locus_tag="VN788_07955"
                     /EC_number="2.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC and FAD-binding oxidoreductase
                     domain-containing protein"
                     /protein_id="HXU20498.1"
                     /translation="MPRLLSVNVGLPRDIEWRGRTVHTGVWKNPVRGRCRVSRLNLDG
                     DGQGDLAGHGGEQRAVFVYQIESYRYWQEQLKRTDFVYGQFGENFTIEGLPDDAVCIG
                     DRYQIGNTVFEVTQPRVTCYRVGIRMNEPRMPALLTSSGRPGFYLRVLQEGEVGAGDE
                     VAKIGDGKERMTVAEINALLYSPNHPRDRLDRALRIEALSPGWRSSFEALLRSPASGN
                     AGLVPEAAAHPVVPGFRPLAVTAIDQESTDVLSLTMASPDGQPLPTALPGQYVVLRLQ
                     PTADGPPLFRSYSLSGSLSTERYRISAKIEPNGVAGNWLREHVRVGDALDISSPRGSF
                     ILQSGERPVVLLSAGIGATPVLAMLHALAAARSTRQILWIHAARDREHHPFVADVRRL
                     MLALPHAHSYVCYSRPGSRDKMGEDFDATGHLSRSVFDEIGVPRDADVYLCGPTRFMA
                     DMKGALALLGVAPERIHVEIFNGSESMTPGVVGAVTKAPHLPAADANTGPLVSFARSG
                     IAAHWNGSAYQSILELAEACDVPVRWSCRTGVCHSCESGLVSGVVVYGPEPLDKPADG
                     NLLVCCSQPTRDVVVDL"
     gene            complement(5554..5838)
                     /locus_tag="VN788_07960"
     CDS             complement(5554..5838)
                     /locus_tag="VN788_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20499.1"
                     /translation="MFVLHVDLKVKQGLEPVIERIYIENFRPAISRQDRFGAVELLRP
                     TEDRGDYRLTIAFEDWTSQQKWVSTEIHQIVWPQMEAQCLSYSVEYYNLV"
     gene            complement(5819..6598)
                     /locus_tag="VN788_07965"
     CDS             complement(5819..6598)
                     /locus_tag="VN788_07965"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU20500.1"
                     /translation="MATTLKTVDTHAHLGECCVFGLISTEEELIRRMDESGIDATIVQ
                     PYPGASQASKTHDRIAGLCEKYPGRIFGLASVSPHGDHDVYRKEVERCVHDLHFVGVK
                     LHTIGHGVNPLSEDGDLVFSTAHELGVPAMVHTGPGVPFALPALCIPAARKYPGLKII
                     LAHAGFAVYSAEAQVAASVCGNLYLETSWCVGEDIRWMISTIGADRVMMGADLPSNVP
                     VEVAKYKALELEPETYSKVMGGTAIEVFKLASRGDYVRAAR"
     gene            6765..>7309
                     /locus_tag="VN788_07970"
     CDS             6765..>7309
                     /locus_tag="VN788_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20501.1"
                     /translation="MMITTNSNPTRRNLFQKVGTAAGAGLVALQSAEPVRAQSSGRPR
                     ALALIGDRYHNADYIRVSLDKVFNGLGISIDYTVQYDRLSAKLLKNYQLFVCLRDGMI
                     WPGGYLGPDAYESYEHDLENETQFVRPEPKFWMTEEQGAAIKDFVMAGNGFYALHNCS
                     HISLTSKNYRDVMGGAYIGHPP"
BASE COUNT         1576 a         2135 c         1971 g         1627 t
ORIGIN      
        1 cgttgccaac tcgcctgaac cataccagcg ctggcccgtt gacgcgatag ctgatgacga
       61 atccggcagc cgcgctcttg tccagattcg ccacgagcag gcctgtttgc tcggtggaac
      121 caccggggtt gggcaagtct cgattgcggc tagacaaatg cagccacttg atctcatgcg
      181 cggcgtgcgg cgtggccaaa accagcgccg caaaagcgta cgataaaagt gctgctgtta
      241 gcccgccgtt ctttgttcga tcagaccaga ttttcacgat tgcctccttg ccgatggccg
      301 tccagccctt ttccgccctg cgcgtttcgg aaaaatcctt gcgtgaaata gtttacgggt
      361 atatcatcgc cccgaatccc aggcaaccgt ccggcaaaaa cgaatatcgc ggcgtgatca
      421 tgcatcgcca gaacagccac aaacgcgctc cagtagcgta tggatccccg ccctgtccaa
      481 gtgtgcagat ttatgcctcc caatccacaa gcgggtggta tcaacgtagc cgcggatttt
      541 ccatcagaga attcgggtca cgcgtgcagt gcttccggaa ggagatactg acattctgga
      601 cggttagtat ataccgtttt aacggagaga tcagcggcgt tgccggggaa gcgtaatgag
      661 tatgaaacga aggcgcttcc atgcgatgcc cgggaacctg aggctcagtt cggcgctgcg
      721 ccccgcagtt tcaccagacg cttcatttgt tccgccacct agtaatcttc ctcgaaggcc
      781 tgagcacacc gtgcacgggt accctccaca ggcagtgaat gattttgaaa cattcttcgc
      841 cattttgttc cggtcgtgca caccttgtac cttgctgaag ggtaagtagt tatacctatt
      901 atggtggccc ggccggagta gcctcatttc atccatggta caggctggga ggagatcaat
      961 gcagggaatg cttcacggca aaagtatggt gaagaccctg cagttggctc cttgtttggt
     1021 ggccgccctc tcccgatcgt atcgtttttc ttttcgcgtg gatgctcgat aaattgggtg
     1081 tggatggacc aaaggccgtc tttgaccggt gcaggctcct cagcgtgaat gggtccgact
     1141 ggaatcagca acaccgcaga tacccagaaa atcttcatct tttcctcctg tatttatctt
     1201 tgacgtcctt catacctgag agtgctgtcg atgactgacg cgctcaccga agacgaaaag
     1261 ccggcgtgga cggggaatac gtcatcctct acgcacaaac ggaaaaggaa tttatggaat
     1321 atctgatcgg ggttggactg gcactggtgg tgtgtgcctt cgcgacgttg gccggatttg
     1381 atcgcgaccg cgtcttctat ccgacgctgc tggccgtgat tgccacctat tacattttgt
     1441 ttgccgtcat ggggaactcc acgctggccc tggccatcga gtccgcggtg gcgggcacgt
     1501 ttcttgtgct ggctgtggca ggcttcaaga ggagcctatg gttggttgta gccgcgctcg
     1561 ccggacacgg agtattcgac ttctttcatc aactgttcat tcagaacccg ggtgtcccaa
     1621 tgtggtggcc cggattctgc ctttcctttg acattctcgc cggtagtttc ctggccatgc
     1681 tgctgatgcg gcgttccgga ttcgcactgg cgacctagaa ttgcggcaca gtgttggccc
     1741 ctcgtgacct ggaatacgcg cttaactggc caatcatgat gcccacgtgg gcgtaacgtg
     1801 atcagaatgt agaaattgaa ggcgcgtgca aagattggac cagttaagcc gaaaataacc
     1861 gccaaactgc ttgtctatta aagcaattac gctgagtcta aagttcaacc agatttggtc
     1921 gagcacgaag gcgaggtata tgccaccaat gacgactgcc accagccccg catcaacgcc
     1981 acaacgaatc cctgctcaga tgtccagacg aacgagaagt cttcagggaa tatgccttta
     2041 gaaccaaata gttaaagatt gaggtgcaat tttgggtgga catctgggga catctgcgga
     2101 cgtagccgag gatctaggtg tccagaccgt caattccctt taattcagtt ccattcggtt
     2161 gaatgtcctc tttttccgtt cgagtacgcc tatcataatt gaaattgaaa ccgtggtcag
     2221 tgcgggagcg cgctgccgac gacgctgaaa gaggatatcg acggagcttg agaagcgaag
     2281 cgtatgcgcc ccgatctcat tcctgagtct acaatatcgt tcagcccgtg ttggaccgca
     2341 tgaatgcatg atggcataat gacgcaaagc caaagccgat ggcttgagag attctcggac
     2401 ggagtatttg gcgtcatcat catttgtctg tctgaagcca caggactcga gcttcacttc
     2461 gctccgtttc gctctcaccg ccggcgagta tccgccttag cgtgattacg ctcgatccac
     2521 gtcttgctcg ctccaaggag ctgctatgag tgaagaaaag ataaccgcag atcggctggc
     2581 tgcatattca gacgcggtct tcgcggtgat cgtgaccatc atggtactgg agctcaaggc
     2641 acccgatcag ccgggattcc cggatctctg gccgctgtgg cccactggca tcagctatgt
     2701 tgtgagctat cttttcattg ccattatttg gataaaccac cattaccttt ggcggttcgt
     2761 tggtcctccg accctgggat tgatatggat caatttcgtt cacctcttca tggtgtcgct
     2821 cctgccgttt gcgacagcat ggattgcgcg cacacgcctc gcatcctccc caatggtgtt
     2881 gtacgcgggg ttatttgtat gtatcgacat tgcctacaat gtcttcgagc accgagtgtt
     2941 ggccggcgcc gacgtaacgc aggtgtcggc gcgtatgcgc cgcatggcta ggcgtcgatc
     3001 actcgccgtc ttagcgagct tcacggccgc tatgctcgtt gccttcgtgg cgccacgggt
     3061 aggcttcggg ctgatatgcg ccgcgctcgt cctccatttg aagcccgata tctcgggtac
     3121 tcggccgtaa cagcggccaa cgccgcctcc tttactgcct gagtcttgat caccgcagac
     3181 cgtcaatcga aaggggccct ggcccttgaa gcgcaagtat caggagggcg gcgagataca
     3241 agagatcgag ttcatagccc acaggaccga accgggcacc cgaagcggaa acactcagta
     3301 gcttcaccga gctaaatccg tacggcagat ggacagtgat cattgcgacc agcaaaacca
     3361 aagcggtggg aatgctcgcc cagaccacga aagctccgag caataaagcc aggcccccga
     3421 caagctccgt gccgatcgtc agccatgtcg ctacgtgtgg cagcggtacg cccatttgct
     3481 gcaaaatccc agcaaatgca tctgcgcctc tcgataactt agcgaagccg tgttcaatga
     3541 agccatagcc gacaagaagg cgcattggaa ggacggccca gcgggcgagg tcagatcgtt
     3601 gcatacactt ctccatgccg atgggccaac aatgaagccg tcggccgccc ctccgacttg
     3661 gcctttgccg ccgctcacag ccgcagtcct tccagactgt gctacataca caatctcttt
     3721 tgatgctctt tgcgttttca tcacaaatcg acgaccacgt cgcgagtggg ttgtgagcag
     3781 caaactagga ggttgccgtc ggcgggttta tcgagtggct ccggtccgta gacgaccacc
     3841 cccgaaacca aaccgctctc acagctatga caaacgccgg tccggcacga ccaacggacc
     3901 ggaacatcgc acgcttcagc cagctccaga atgctctgat aggccgaccc gttccagtgt
     3961 gcagcgatgc cgctgcgcgc gaatgatacc agcggacctg tgttcgcatc ggccgcgggc
     4021 aaatgaggag ctttcgtcac ggcgccgacg acaccaggcg tcatcgactc gctgccgttg
     4081 aagatttcga cgtgaatccg ctccggcgcg acgcccagga gtgccagcgc ccctttcatg
     4141 tctgccatga agcgagtcgg cccacagaga tagacatccg cgtctcgtgg gacgccgatt
     4201 tcatcaaaga ccgatcgcga caggtgaccg gtagcgtcga aatcctcccc catcttgtcg
     4261 cgcgagcctg gtctgctgta gcaaacataa ctgtgggcgt gcggtagcgc gagcatgagg
     4321 cggcggacgt cggcgacgaa tggatgatgc tcccgatcgc gagccgcgtg tatccacaag
     4381 atttgccgtg tcgatcgggc tgccgccagc gcgtgtaaca tcgccagtac aggcgttgcc
     4441 ccgattcccg cgctgagcag cactaccggc cgctctccgg actgaaggat gaaacttccg
     4501 cgcggcgagc tgatgtcgag agcatcgcca acgcggacat gttctctaag ccagtttcca
     4561 gccacaccat tcggctcgat cttcgcgctg attcgatagc gctccgtcga aagggaaccc
     4621 gaaagcgagt agctgcgaaa aagcggcggg ccatcggcgg tcggctgaag acgcaacacc
     4681 acatactggc cgggcagagc cgttggtagg ggctgaccgt ccgggctcgc catcgtcaac
     4741 gagaggacgt ccgtggactc ctgatcgatc gccgtcaccg cgagcgggcg aaatcctggt
     4801 acaaccgggt gcgcagccgc ttccggcaca agccccgcat tcccacttgc cgggcttcgc
     4861 agcagtgctt caaatgacga gcgccatccg ggcgaaagcg cttcgattcg caacgcgcgg
     4921 tccagtcgat cgcgcggatg attaggcgaa tagaggagcg cgttgatctc tgcgacagtc
     4981 attcgctcct ttccgtctcc gatctttgcg acttcgtcgc cggcacccac ctcaccctct
     5041 tgcaggacgc gtaagtagaa cccaggcctt ccgctggatg tcagcaatgc cggcatccgg
     5101 ggctcgttca tccgaatgcc cacgcgatag caagtgacac gaggttgagt gacctcgaac
     5161 acggtgttgc cgatctgata acggtcgccg atacacaccg catcatcggg caatccttct
     5221 attgtaaagt tctcgccgaa ctgtccgtag acgaagtcgg tcctcttcag ttgctcctgc
     5281 caatatcgat acgattcaat ctggtaaacg aagacggcac gctgctcacc gccgtgccct
     5341 gccagatcgc cctggccgtc tccgtccaga ttcaagcggc tgaccctgca gcggccacgc
     5401 accggattct tccagacccc ggtgtgcaca gtccggcctc tccattcgat gtcgcgtggt
     5461 agaccgacgt ttaccgacag aagtcgaggc attttcctcc gcaggggctt cccgccgctc
     5521 ccggtcaaac tagaacactt actaactggc ctactaaact aaattatagt actcaacgga
     5581 atagcttaag cattgggctt ccatttgcgg ccagacgatc tgatgaattt cagttgatac
     5641 ccacttttgc tgggaagtcc agtcttcgaa cgcgatggtc agccggtagt cgccgcgatc
     5701 ttcggtcggc cgcaacaact cgacggcacc aaacctgtct tggcgggaaa tggccggtcg
     5761 gaagttttcg atatagattc tctcgattac cggctccaac ccctgcttta cctttaagtc
     5821 aacgtgcagc acgaacatag tcacctcgcg aggcaagctt gaatacctcg atagcggtac
     5881 cacccatcac cttggagtaa gtttcgggtt ccagctccag cgctttgtat tttgcaacct
     5941 cgacaggaac gttactcggc aagtccgctc ccatcatgac tcgatccgcg ccaatggtgg
     6001 atatcatcca tcggatatct tctcccacgc accaggacgt ttcgagatac aggttgccac
     6061 aaaccgaagc cgctacctgc gcctccgccg aatagacggc gaatccggcg tgcgccagta
     6121 taatcttcag gccgggatac ttccgggccg ctgggataca gagcgctggc agcgcgaacg
     6181 gaacgcctgg gcctgtgtgg accatcgccg gaactcccaa ctcgtgcgcc gtgctgaaaa
     6241 caagatcgcc atcttccgac agtgggttca ctccatgacc aattgtgtga agcttgacgc
     6301 cgacgaaatg cagatcatga acgcatcgtt ctacttcctt ccggtacaca tcatggtctc
     6361 cgtgcgggct cacgctagcc aatccaaaga tacgtccagg atatttctcg caaagcccgg
     6421 cgatgcgatc atgagttttg gaagcttggc tcgctccagg atacggctgc acgatcgttg
     6481 cgtcgatccc gctctcgtcc atccggcgga tcaattcctc ttcggtgctg attagcccaa
     6541 aaacgcagca ttctcccaag tgtgcatgag tgtcaactgt ttttagcgtc gttgccattt
     6601 caacgtctcc ataacgtctg caatctctcg ttccggatat cgcgatcttt agagtaccgt
     6661 gcagaaggta aaacacggag aaccgcgaaa gttccccggg gttgacttgt ctgtgcttcg
     6721 cgcataaact ccttttgaag atcggttaca aggcgcgctt ccgcatgatg ataaccacta
     6781 actccaatcc cacgcggagg aatctttttc aaaaagttgg cacggcagcg ggagccggac
     6841 tggttgcttt gcaatccgct gaaccggtgc gcgcgcaatc gagcggacgg cctcgcgcac
     6901 tcgctctcat cggagatcgt tatcataacg ccgattatat tcgggtcagt ctcgataagg
     6961 tgttcaacgg ccttggaatc tcgatcgact acaccgttca atacgatcgg ctctccgcca
     7021 aactgctcaa gaattatcaa ctgttcgtct gcttgcgtga tgggatgatc tggcccggag
     7081 gttaccttgg gccggacgcg tacgagtcct acgagcacga tctcgaaaac gaaacccagt
     7141 tcgtgagacc agaaccgaag ttctggatga cggaggaaca aggcgcggcg atcaaggatt
     7201 tcgtcatggc gggaaacggt ttttacgcgc tacacaactg tagccacatc tcgctaacct
     7261 cgaagaatta ccgggacgtg atgggtggag cttacatcgg ccaccctcc
//
LOCUS       DATNDT010000248         4350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_311871, whole genome shotgun sequence.
ACCESSION   DATNDT010000248 DATNDT010000000
VERSION     DATNDT010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4350
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311871"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..799
                     /locus_tag="VN788_07975"
     CDS             <1..799
                     /locus_tag="VN788_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase C45"
                     /protein_id="HXU20502.1"
                     /translation="DGFPGAIASDDDFGVNSAGMMITETTMANFHGWDPNGKPEFVRA
                     RKAMQYADSIDSYVKIMLDGNNGGYANDWLLGDRKTGEIARFELGLEHTKVWRTKDGY
                     FSGANFASDPDVIRDETTFDPLNPATSMNARKLRWDQLLNKNKGVIDTGLAEVFLADH
                     YDTYEKKEDPNRRTLCGHGDNTPITLPDSTSAPFTPSGAVQAKVIDSAMASNMQLTAR
                     IGHPCGTNFDAAKFLSEHPEFSWESPVLRDMIAGPWAQFRVGEHPAQ"
     gene            complement(823..1407)
                     /locus_tag="VN788_07980"
     CDS             complement(823..1407)
                     /locus_tag="VN788_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXU20503.1"
                     /translation="MRGYNSSSLRVSVLVVLLGFFVVALGATGSSLAAQASHENRVPD
                     QESVTREVRHQLVMLPFYTLFDNLEYKVEGSKVTLMGEVVRPNLKDDAGRAVKHVRGV
                     ETVDNQIKVLPLSPMDDQLRRAEYRAIYSQPNLQMYAMQSVPPIHIIVDHGHVTLEGV
                     VAKQADKDVAGIAAKGVPNVFSVTNNLRVEGGAK"
     gene            1644..1916
                     /locus_tag="VN788_07985"
     CDS             1644..1916
                     /locus_tag="VN788_07985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20504.1"
                     /translation="MLHFTLFIEDECAEMRRETALGKASHDRQIEFMDRIAGRGEALV
                     HTATSGFAMPDELKARILSTLLTLMNTRENMDRAAMRQSAVRRFAG"
     gene            complement(1930..2973)
                     /locus_tag="VN788_07990"
     CDS             complement(1930..2973)
                     /locus_tag="VN788_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002644961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXU20505.1"
                     /translation="MKPEVYEKYSRQILFAEIGEGGQEKILNSSAVLVGCGALGTALA
                     GLLVRSGLGKLRIIDRDFVELSNLQRQTLFDEADAHGALPKAIAAERHLRAINSDADV
                     EGIVADLTPQNALELLSGFPLILDGTDNFQTRLLLNDAAVSLGIPWIYAAVVGSYGVT
                     MTIRPSETACLACLLESGEPSGAYSLGNELTCDTAGVLGAAAGVIASIESSEAIKILS
                     GRLDALHRRLISCDVWTGKFQSIRTEHNPSCRACVRREFRFLAGDAQPHITLCGRDSV
                     QIHERGRQLNLDDLSRRLRGAGSAQVQSNDFLLRFCVSSYEVTVFSDGRAIIKGTKDP
                     AVARSLYARYIGT"
     gene            3125..>4350
                     /locus_tag="VN788_07995"
     CDS             3125..>4350
                     /locus_tag="VN788_07995"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXU20506.1"
                     /translation="MRSYREHVLRLAPHFAGRMTALAALLLSLSALFPSATCSQESAV
                     EGLKVARVRVVDKGGAQVPQVIPKLPLETGGRFDFAAERQSVREIYKMGDFSDVRVGV
                     ERQDEGVEVDFIVTLNFYNNVIRIDGLKPPPSEPAALAALRLGLGEPYRESALREAIG
                     RLQDSLRDDGFYQAKITWALSPHEDTRQMDITITVDSGPRARLGSISVQDNTRYTKEE
                     LLRKSKLSPKNEVTSARLSRATERIRKFLVNQGYLGAGAVISRGNYDSRTNQVPLTLS
                     ITAGSRVRVEIVGTRLSKSKRRALLPVFAEGAVDDDLLQEGRRNIRDYLQSLGYFNAN
                     VQVSSHQDDRKHEQVITYQVARGDRFRLAGISFEGNKYFNSDLLSRRLALQPASFASS
                     GRFSQQLLRGDTDSIRA"
BASE COUNT          979 a         1218 c         1250 g          903 t
ORIGIN      
        1 ggatggcttt ccgggagcga ttgccagcga cgacgatttc ggcgtgaatt ccgccggaat
       61 gatgatcacc gaaaccacca tggccaattt ccacggctgg gatccaaacg gcaagcccga
      121 gttcgttcgc gcccgcaagg ctatgcagta cgcggattca atcgacagct acgtaaaaat
      181 catgctcgac ggaaacaacg gcgggtatgc aaacgactgg ctgctcggcg accgtaaaac
      241 cggcgaaatc gcgcgattcg aattgggcct cgaacacaca aaagtatggc gcaccaagga
      301 tggctacttt tcgggagcca atttcgccag tgatccggat gttatccgcg atgaaaccac
      361 cttcgatccg cttaatccgg ccacttcaat gaacgctcga aagctccgtt gggatcaatt
      421 gctgaacaaa aataaaggag taatcgatac agggctggcc gaggtttttc tggcggacca
      481 ctacgatacg tacgaaaaaa aggaagaccc caaccggcgc acgctttgcg gacacggaga
      541 caatacaccg ataacccttc cggattcgac ctctgcgccc ttcacaccaa gcggcgctgt
      601 acaagctaaa gtgatcgata gcgccatggc ctccaacatg caactcacgg cgagaattgg
      661 ccatccttgc ggaaccaatt tcgacgccgc gaaattctta tctgaacatc ccgagttttc
      721 gtgggagtcg cccgtgctcc gcgacatgat tgccggtccg tgggcgcagt tccgcgtcgg
      781 cgagcatccc gcgcagtgaa ccgcccgtga aggggcgcca aattacttgg cgccgccctc
      841 aacgcgcaga ttgttggtaa ctgaaaaaac gtttggtacg cctttggccg cgatgccggc
      901 aacatctttg tccgcctgct tggccacgac gccttcgagc gtcacgtggc cgtgatccac
      961 gataatgtga atcggcggca cgctttgcat agcgtacatc tgaagattcg gttgggagta
     1021 gatggctcga tactccgcac gccgaagctg atcatccatc ggtgagagcg gcagcacctt
     1081 gatctggttg tcaactgtct cgacgcctcg gacgtgcttt accgcacggc ccgcatcatc
     1141 cttcaggttc ggcctgacaa cctcgcccat cagcgtaact ttgctgcctt ctaccttata
     1201 ttcgaggtta tcgaatagcg tgtagaacgg aagcatgacg agttggtggc gcacttcgcg
     1261 ggtaaccgat tcctggtctg ggacccgatt ctcatgggaa gcctgggctg ccagactcga
     1321 ccccgtggct cccaacgcaa ccacgaaaaa ccctaaaagg accaccagaa cagagaccct
     1381 tagtgaactt gagttgtagc ctcgcatcga atcctccatg gcgacctcag taggggcaaa
     1441 aggcccgcta aatcgttaga tgccaaagcc ccggttttgg actgctatct gataagcggc
     1501 atgcgaaaaa tgggaatctg gaaaatccct ttggaaggtg ctacaaaaca aagtgcttga
     1561 ctgggtgtgt gatgccagaa gggtgctcct cgaagggctg taaaaacgtg gttcccgcgg
     1621 gactcaccgc agagcacatg tgtgtgctcc atttcacatt gtttattgaa gacgaatgcg
     1681 ccgagatgcg ccgcgagaca gcgctcggta aagccagcca cgatcggcaa atagaattca
     1741 tggataggat tgcggggcgt ggcgaggcgc tggttcacac ggccaccagc ggctttgcga
     1801 tgcccgacga gctcaaggct cggatcttga gcacactttt gacattgatg aacacccgcg
     1861 agaacatgga ccgcgccgcc atgcggcaat ccgcggtcag gcgttttgcc ggttaactat
     1921 ttctcccctt caggttccta tgtagcgagc gtaaagagag cgcgcgacgg ctgggtcttt
     1981 tgtgcctttt atgatggcgc ggccatccga aaacacggtt acttcgtagg atgaaacaca
     2041 aaaccgcaga aggaaatcgt tactttgtac ctgcgcgctt ccggcgccgc gaagacggcg
     2101 gctcaaatca tcaagattga gttgcctgcc cctttcatgg atctggacgg aatcacgccc
     2161 gcacaacgtg atgtgcggct gagcgtcgcc cgccaaaaaa cggaattcac gccggacaca
     2221 agcgcggcag gatgggttgt gctcggttcg gatcgactga aattttccgg tccagacatc
     2281 gcaggaaata agccgtctat gcagcgcgtc cagccggccg gacagaattt tgattgcctc
     2341 gcttgattcg atggaagcga tgacgccggc cgcggcaccg agcacgccgg ccgtgtcaca
     2401 agtgagttca ttgcccaggc tgtaggcgcc actcggctcg ccgctttcga gcaagcaggc
     2461 aagacaagcc gtttcagatg gccgaatcgt catggtgacg ccgtaacttc cgacgaccgc
     2521 ggcgtaaatc caagggatac cgagcgaaac tgcggcgtca ttcagcagaa ggcgcgtttg
     2581 gaaattatcg gtcccgtcca gaatgagagg gaatcccgaa agaagttcga gcgcgttttg
     2641 cggagtgaga tcggcgacga ttccctcgac atccgcatcg gaattgatag cgcgcaggtg
     2701 gcgctcggct gcaatggcct tgggcagagc gccgtgtgca tctgcctcat cgaaaagcgt
     2761 ctggcgctgc aagttggaga gttccacaaa atcgcggtcg atgatacgga gctttcccaa
     2821 ccctgaccgg acgagcaggc cggccagtgc agtacctagc gcgccgcagc cgaccaggac
     2881 agcgctggaa ttgaggatct tttcctgccc gccttcaccg atttccgcga agagtatctg
     2941 tcgcgaatat ttttcgtaaa cttcgggttt cattcaaggc aatcccaaat actgcgccgg
     3001 cccttgcgga tgaactttcc aagagagatt cttgcgtttg gccatccgcg tttatcatat
     3061 cgccttttca cacggggcat acaaccgggc tgtgctaacc tttgctctta aattttttgg
     3121 cggaatgaga tcttaccgtg agcatgtgtt gcgtttagcg ccgcatttcg cggggcgcat
     3181 gacggcgttg gccgcgcttc tcctttcctt gagcgcctta tttccctccg cgacgtgttc
     3241 ccaagaatcc gcggtcgagg gattgaaagt cgcgcgagtt cgcgtcgttg acaaaggcgg
     3301 ggcgcaggtt ccccaggtaa tacccaagct cccgctggag accggcgggc ggttcgattt
     3361 cgcggctgag cgccaaagtg tccgcgaaat ctacaagatg ggcgactttt cagacgtccg
     3421 cgtgggggtt gagcggcagg acgaaggcgt tgaggtagat ttcatcgtca ccctcaattt
     3481 ctacaacaat gtgattcgca tcgatggact gaagccgccg cccagcgagc cggcagctct
     3541 ggcggcgctg cggcttgggc tgggcgagcc ttaccgcgag agcgcgcttc gagaggctat
     3601 cggccgtttg caggattctt tgcgagacga cggattctac caggcgaaaa ttacttgggc
     3661 tctgagcccg catgaagata cccggcagat ggatatcacg ataaccgtgg attccgggcc
     3721 gcgggctcgc ctgggtagca tcagcgtgca agataacacc cgttacacca aagaggaatt
     3781 gttgcggaag tccaaactgt cgccgaagaa cgaagtaacg tctgcccgcc tttcgcgtgc
     3841 tacggaaagg atcaggaaat ttctggtgaa tcaaggctac ctgggagcgg gcgctgtaat
     3901 ttcccgaggg aattacgatt cgcgaacaaa ccaggtgccg cttaccctat cgatcacggc
     3961 agggtcgcgc gtgcgggtgg agatcgtggg cacgcggttg agcaaaagca aaaggcgggc
     4021 gctcctaccc gttttcgcgg aaggcgccgt ggatgacgat ttgctgcagg aaggccgccg
     4081 caatatccgc gattatctgc agagtttggg ttacttcaac gcaaatgtcc aggtcagctc
     4141 gcatcaagac gatcgaaagc acgaacaggt gatcacctat caggtcgcga gaggcgaccg
     4201 ctttcgcctc gccggcatct cctttgaggg gaataaatac ttcaacagcg acttgctctc
     4261 tcggcgactg gcgcttcagc cggcttcgtt tgcctccagc gggcggttca gccagcaact
     4321 cctgcgaggc gatacggact ccatccgagc
//
LOCUS       DATNDT010000249        11324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2326073, whole genome shotgun sequence.
ACCESSION   DATNDT010000249 DATNDT010000000
VERSION     DATNDT010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326073"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..572)
                     /locus_tag="VN788_08000"
     CDS             complement(<1..572)
                     /locus_tag="VN788_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HXU20507.1"
                     /translation="MVKESSIRVSFTEELQDGAKIKVIGVGGGGSNAVNRMIRAKVEG
                     VEFIAANTDLQALKLSQSPVKLQLGAKLTKGLGAGANPEVGRKAALEDTEKILEALDG
                     ADMVFVTSGLGGGTGSGAAPVVANLASELGALTVAVVTKPFAFEGKRRMQQAEQALAE
                     LIGCVDTVIVIPNERLMEYVEHGTSFFEAFR"
     gene            complement(663..1877)
                     /gene="ftsA"
                     /locus_tag="VN788_08005"
     CDS             complement(663..1877)
                     /gene="ftsA"
                     /locus_tag="VN788_08005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HXU20508.1"
                     /translation="MTKKERHIVALDIGSTKTCALIGEMEDDGSVKFAALGAAESKGW
                     RKGQIVNLDLAVSSIRRAVEEAETVVGVPVESAMIGVAGGHVRGVNSRGGVTLGTRPR
                     DVQREDVRRAIEAARGVTLPEDREVIHVLPQEFFLDAQDNIRDAIGMVGHRLEASVHI
                     VTASGAATQNIVTAVNRAGVRVDDTVLEPFAAAEACLTQDERELGCCLLDIGGGTTEM
                     VAYTGGVVRHTGAIPIGGDHFTNDLAVGLRTPVPEAEKIKREHAQACRELLTEDIPIE
                     IASVGDRPPRTVFTRMVGEIIGARAQELLMLIRDELRRSGLQSQIPAGIVLTGGGSQL
                     RGLVELAERLFNLPVRIAVPRGLGQMSEEVSRPEYATAVGLLVYGARTRRFAGARPTG
                     FIGKLKSMFAGA"
     gene            complement(1887..2870)
                     /locus_tag="VN788_08010"
     CDS             complement(1887..2870)
                     /locus_tag="VN788_08010"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HXU20509.1"
                     /translation="MAKLKSIDIETYPREILVDEEPKYLRRQKPLEIKRRKFGRKVWK
                     TYLQVTMWAAVGVVGAWAVYDFSHFLLTSPETALIHPEQIVLAGNHYVAPASVLGIFA
                     PDRGKSILLIPLDERRRQIEALSWVERATVRRVLPNQIAVEITERIPIAFLREPGDLA
                     LIDAHGVILSRPIRGNFHFPVVTGIMAGMPVDSREERMQLFSGFVQQIESVRPDAIDR
                     ISEVDLSDGHDLRATLTGFEPTVANTVTPDSQTQMHVDTPVLVHFGDTDFAGKYQTLV
                     EEFNDWRAEAGAIESVDLRFSREAVVNQIPVTLARRQPPKLAATSTRRHSR"
     gene            complement(2858..3763)
                     /gene="murB"
                     /locus_tag="VN788_08015"
     CDS             complement(2858..3763)
                     /gene="murB"
                     /locus_tag="VN788_08015"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HXU20510.1"
                     /translation="MRINDTVSARLEHLGAELRHGVSLMERTSLGIGGITDEILFRRY
                     ESIPDVMTLLADESIPHRFLGGGTNVLIADGELPYIVLHLATLHPGMRIEGNAAYIDA
                     SADLGGMVTFCAKRDLGGMEGLIGVPGSVGGALRMNAGAYGTSIGPHVREIELYRAAS
                     GELEKVRGTDIRFDYRHTSFAPDDVMLRVKLELPPRPFKEIVAGIKVCNEKRRASQPL
                     NQKSAGCIFKNPPGGSTGRMIDELGLKGHRVGDAMVSERHANFFVNAGSASCSDMLRL
                     IDDVRERVHGSYGVDLENEVIVWQN"
     gene            complement(3753..5159)
                     /gene="murC"
                     /locus_tag="VN788_08020"
     CDS             complement(3753..5159)
                     /gene="murC"
                     /locus_tag="VN788_08020"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006001972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HXU20511.1"
                     /translation="MNITFRNFQRIHLVGIGGSGMSGIAEVLLSSGYGVSGSDLKSSP
                     ATQRLRKLGAVIHDTHKAENAHGAHVLVVSSAVPGDNVEVAEAHRLKIPVIPRAEMLA
                     ELMRLKYGIAVAGAHGKTTTTSMVASILAAADLDPTFVVGGRVNHIGANAAVGRGEYM
                     VVEADESDRSFLLLAPVIAVITTIDREHLDQYSSLEEIQEVFLQFANRVPFYGAVVLC
                     LDEPNAQAIVPGIKRPIISYGTSSQADLVISDVKLSGLSSQFSLKYRGEDLGVFRLPA
                     PPGIHNVRNAAAAAAAALELNIPADLIREGLTHFSGVGRRFEIKGDFSGVTLIDDYGH
                     HPAEIRATLEAARGCGYKRLLVLFQPHRYTRTQHLWEDFRLSFNQADILVLTDIYAAG
                     EPPAEGITGELLAEAISSAGHKSVVYSSTLQGGIEYILREARPGDAVLTIGAGSVGRV
                     TEELAVLLGSRVSIFNAD"
     gene            complement(5156..6256)
                     /gene="murG"
                     /locus_tag="VN788_08025"
     CDS             complement(5156..6256)
                     /gene="murG"
                     /locus_tag="VN788_08025"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HXU20512.1"
                     /translation="MSADAKRLLIAGGGTGGHVFPALAIAKEWIRRGADRGESERQVV
                     FVGTERGIETRLVPQACFPLELIRASGLKGIGGVKLLRNAAMVPAGLWDSEKILHRYR
                     FNAAFGVGGYASGPMMLLAAMHRVPTVVFEPNVEPGFTNRVLAAIATKIAVAHQETAG
                     RLGSRATVTGCPIRPEFFAIPSKDHRPPFTLLFTGGSRGASPINRAVVDSLDILATKK
                     DGLFIVHQTGERDYNAVREAYDRRQFQAEVVPFIENMAERFAQADLIVCRSGAITVAE
                     VSASGRAAIFIPFGASTDAHQTGNARVMASAGASRLLPQDELTPERLTNEIFFLLDQP
                     QRVQEMEDRARSLGRPRAVEDIVNVIDGVARP"
     gene            complement(6253..7362)
                     /gene="ftsW"
                     /locus_tag="VN788_08030"
     CDS             complement(6253..7362)
                     /gene="ftsW"
                     /locus_tag="VN788_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HXU20513.1"
                     /translation="MPRNLQHDRKLFGATLALCLIGAVMVFSASAVTLREQGGNGYEF
                     LLRQLLWATFGMSGMFLLMNTDYRKLRQPRVIFTAVSVTLILLLAVFFLDRTHATHRW
                     VRVGPLSLQPSEIAKLVVIFYLAWFLEIRREPRGPGVNNLTQTLLPVLGAVLLIVALV
                     YKEPDMGTACMIFFIAAVMLFIAGLSYRYIFGAAIAAIPLVYIAIASVPYRLQRVQAY
                     FSPGADPQGHGFQLAQSLIAVGSGGFNGVGLMESRQKLFFLPEAHTDFIFSVICEEAG
                     FIGAIIVLTLFAVYGWRGFVAAMKAPDAFGRFLGIGITTMVVGQALFNLSVVLGLVPT
                     KGIPLPFISYGGSSLMGMLLATGVLLNISQHSDET"
     gene            complement(7365..8852)
                     /gene="murD"
                     /locus_tag="VN788_08035"
     CDS             complement(7365..8852)
                     /gene="murD"
                     /locus_tag="VN788_08035"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HXU20514.1"
                     /translation="MGRVENHRPILDHCARAGTFFTHDAEIKVGQMRPGFDLEGKRVL
                     VVGLARTGLVVSLFAAERGAVVTATDMRTESSLAETAPQLRDAGVKLELGTHLPSTFA
                     EQDLIVVSPGVSPNLPALKLATSLGIPVWSEIELAWRFLRGKLIAVTGSNGKTTTTSL
                     ISHILRTGRISTLMGGNIGVPLLSLVESATDSTITVAEISSFQLETIETFRPEIGVLL
                     NLTPDHLDRHVSFEEYATAKVRLFENQLDRDFAVLNADDPEVTKRAPAVPHIFWFSRR
                     KRLAQGAFLQGNQITIRNEGMELAVGRRDEISLRGEHNLENVMAACAAAHLAGAAPAD
                     IAVGVKTFRGVEHRLELVGKVNSVEFYNDSKATNVDAALKAIEAFAGPLIVILGGKDK
                     GGPYIPLQEPLRRRARRVILIGSAAEKIASDLGVGLPILQAGTLDRAVQAAIEQAQPG
                     DVVLLAPACSSFDQFENYEHRGRVFKQLVQDFARASNENVFARKG"
     gene            complement(8765..9898)
                     /gene="mraY"
                     /locus_tag="VN788_08040"
     CDS             complement(8765..9898)
                     /gene="mraY"
                     /locus_tag="VN788_08040"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HXU20515.1"
                     /translation="MLYYFFFFMLRRHFSPLNVFRYITVRTAAASLTALLLSLLLGPW
                     IIKRLRSLQVKQYIREEGPQRHQAKAGTPTMGGLLILAAIVIPTLLWADLRNPYVVLA
                     VVATLAFGAIGFVDDYNKVIRKQNRGLTGRGKFALQVLTCVFVGAVLIGLQAKGVYST
                     QLSVPFVKGLHPDLVIGSLLDSHLIWPLAYLPFVLFLVLVIAGLSNGVNLTDGLDGLA
                     IGCVLISAVALTVLTYLSSNSRFAEYLDIQRIPDAAELTIFCGSLAGASLGFLWYNAH
                     PAEMFMGDVGSLALGGAIGTVAVILKQEILMISIGGIFIVELLSVVIQVASFKLTGKR
                     VFRMSPLHHHFELVGWGESKIIVRFWIIALVLALFSLTTLKLR"
     gene            complement(9900..>11324)
                     /gene="murF"
                     /locus_tag="VN788_08045"
     CDS             complement(9900..>11324)
                     /gene="murF"
                     /locus_tag="VN788_08045"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXU20516.1"
                     /translation="QRIEPSAIIGGVSIDSRTVGAGELFVAIRGPRHDGHAFVASALE
                     RGAMAALVARERFLNYPDEIRGKLFAVDDTLRGLQDLARHACQSWRKARGGRLIGAVA
                     GSVGKTTTKEILAALLGARYRVLKTQGNLNNEYGLPLTLLKLSDEHGAAVVELGMSHS
                     GELAELTRICRPDVGVVTRVAVEHLEFFPSLEEIALAERELIENLPWPNATAVLNSDD
                     ERVARFAEIAKGPVIRFGTREGAEFQAEAIKERGIEGSEFEFVSRGRRVRLQLPLIGR
                     HNVMNALAALAAASVWGIGTNEAKEVFPSLTAADKRGEVVRFEDGFSVINDTYNSSPT
                     ALSALVTLLAQTPGYRRRILASGEMLELGSSSPELHKECGRLAAALRNIDWIVGVQGH
                     AADFVAAAIQAGHPQERTAFFQNSTEAGKFLSEFVMSGDLVLLKGSRGVRMEKVLEAI
                     NAHHRPSAQNPGLQHVDTGREGRN"
BASE COUNT         2541 a         3424 c         3063 g         2296 t
ORIGIN      
        1 cgaaatgctt cgaagaagct ggtgccatgc tcgacgtatt ccatcagccg ctcgttggga
       61 atgacgatca cggtatcgac gcagccgatg agctcggcga gagcttgttc ggcctgctgc
      121 atgcggcgct tgccttcgaa cgcgaacggc ttggtaacga cggcaactgt aagagcgccg
      181 agctcgcttg ccagattcgc caccacgggc gccgcaccgc tgccggttcc accgccgaga
      241 ccggaggtga cgaagaccat gtccgcgccg tcgagggctt cgagaatttt ttcggtgtcc
      301 tcgagcgcgg ccttgcggcc tacttcggga ttggcgccag cgcccagccc cttggtcagc
      361 tttgctccga gctgaagctt cacgggcgat tgcgaaagct tcagagcctg cagatcggtg
      421 ttagcggcga tgaattcgac gccctcgacc ttggcgcgga tcatgcggtt gacggcgttg
      481 gaaccaccgc cgccgacccc gattaccttt attttcgcgc catcctgcaa ctcttcagta
      541 aacgaaacgc gaatgctcga ctcttttacc atgctgcctc cccagtggta caaagttaat
      601 tttttgatcg gcggcggatc cagttggaga ccgtcgcacc agatttcgtt ttccttattt
      661 cctcacgccc ccgcaaacat gcttttcaac ttgccaatga agcccgtagg acgagcgccc
      721 gcaaagcgcc gcgtgcgcgc tccgtagacg agcagtccca cagccgtcgc atactcgggc
      781 cgggaaacct cttcgctcat ttgtccaagg ccgcgcggca cggcgattcg aacgggcaga
      841 ttgaacagcc gctcggccag ctctaccagt ccccgaagct gcgatccgcc gccggtgaga
      901 acaattcccg cagggatttg cgattgaagg ccgctgcggc gaagttcatc acgaatgagc
      961 atcagcaatt cctgggcgcg cgcgccaatg atttcgccaa ccatgcgcgt gaacacagtg
     1021 cgcggcggac gatcgcccac actggcaatt tcgatgggaa tgtcttcggt tagcagctca
     1081 cggcatgctt gcgcatgctc ccgcttgatc ttttcagctt ccggaacagg cgtgcgcaaa
     1141 cccaccgcga gatcattcgt aaagtggtct ccaccgatgg gtattgcccc ggtgtggcgc
     1201 accacgccgc cggtgtaggc gaccatctcg gtagtcccgc ctcctatatc gagaaggcag
     1261 cagcccagtt cccgctcgtc ctgcgtgaga caggcttcgg ccgcggcgaa cggctcaagc
     1321 acggtgtcat caacgcggac gcctgcccga ttgaccgcgg tgacgatgtt ctgggtcgcc
     1381 gcgcccgatg cggtgacaat atggacgctg gcctcgagac gatggccgac catgccgatg
     1441 gcgtcgcgga tattgtcctg ggcgtcgaga aaaaactcct gcggtaatac gtggatcacc
     1501 tcgcgatcct cgggaagagt gactccgcgg gcggcctcga tggcgcgccg aacatcctcg
     1561 cgttgaacgt cgcggggccg cgtacccaag gtgacgccgc cgcggctatt gacaccgcgg
     1621 acgtgtcctc cagccacacc gatcatcgcg gattccaccg gcacaccgac aaccgtctcc
     1681 gcttcctcga cagcacgccg aatcgagctg acggccaagt cgagattcac gatctggccc
     1741 tttcgccatc ctttggattc agctgcgccg agggcggcga acttcacact gccgtcgtct
     1801 tccatctcac caatcagggc gcaggtcttg gtgcttccaa tgtccagcgc cacaatatgc
     1861 cgttctttct tagtcaaatg ctgctcctat ctcgaatgcc ttcgcgtgct cgtcgcggcc
     1921 aatttcggcg gttgccgccg ggccaaggta accgggatct gattgacaac ggcctcgcgg
     1981 ctgaaccgga ggtccaccga ttcgatggcg cctgcctcgg cgcgccaatc attaaactct
     2041 tcaacgagag tctgatattt gccggcaaaa tcagtgtcac caaaatgcac gagcacgggt
     2101 gtatccacgt gcatctgagt ttgcgaatca ggtgtgacgg tgttcgcgac agtaggctcg
     2161 aagcccgtga gggtcgcgcg caaatcgtga ccgtctgaaa gatcgacttc actaatccgg
     2221 tcgatggcat ctggtcgtac cgactcaatc tgctggacga acccggagaa aagctgcatg
     2281 cgttcctcgc ggctgtcgac cggcatgccg gccataattc cggtcacaac ggggaaatga
     2341 aagttgcctc tgatgggccg cgagaggatc accccatgcg cgtcaatcag agctagatcg
     2401 ccaggctcgc gtaaaaacgc aataggaatc cgctcggtaa tttcaactgc gatttgattc
     2461 ggcaggacgc gccttacagt tgcgcgctca acccaggaaa gcgcctcgat ctggcgtcgt
     2521 cgctcatcca acggaatgag aaggatgctc ttgccgcgat ccggggcgaa aataccgagt
     2581 acgcttgcag gtgcaacata gtgatttccg gccaggacaa tctgctccgg atgaatgagg
     2641 gctgtttcgg gagaagttag caggaagtga ctgaaatcgt agaccgccca agcaccgaca
     2701 acacccaccg ccgcccacat tgtcacctgc agatatgttt tccagacttt gcggccaaac
     2761 ttccgccttt taatctcaag cggcttctgt cgccgaagat acttcggctc ctcatccacc
     2821 aggatttccc gcggataggt ctcgatgtcg atggacttca gttttgccaa acgattacct
     2881 cgttctcaag atcgacgcca taggaaccgt gaacgcgctc gcgaacatcg tcaattagcc
     2941 gtaacatatc cgagcagctc gcgcttccag cattgacaaa gaaatttgca tgccgctcgc
     3001 tcaccattgc gtcacccacg cggtgacctt ttaacccgag ttcgtcgatc atgcggcccg
     3061 tggatccccc ggggggattc ttaaaaatgc agccggcgct cttttgattc aaaggctgac
     3121 ttgccctgcg cttctcgttg cacaccttga ttcccgccac gatttccttg aatggccttg
     3181 gcggcaattc cagtttcaca cgcagcatca catcgtccgg cgcaaaagaa gtgtgccggt
     3241 aatcaaaacg aatatcagtg ccacgaacct tttcaagttc gccggaggct gcgcggtaca
     3301 actctatctc gcggacatga gggccaatcg aggtaccgta tgctccggcg ttcattcgta
     3361 gagcgccgcc cacgctgcct gggacgccga tcagtccttc cattccgccg agatcacgtt
     3421 tcgcgcaaaa cgtcaccatt ccgccgaggt ccgcggaggc gtcgatatac gccgcattac
     3481 cctctatcct cattccgggg tgcaacgtgg cgagatgcag cacaatgtat ggcaactcgc
     3541 cgtcggcgat cagcacgttc gtaccaccgc cgagaaagcg gtgtgggata ctttcgtctg
     3601 caagcaacgt catcacatct ggaatcgatt cgtaacggcg gaataggatt tcgtccgtga
     3661 ttcctccaat gccaagggaa gtcctctcca tcagagaaac accgtggcgc agttcagcgc
     3721 caagatgctc caaccgagcc gacactgtgt cattaatccg cattgaaaat cgaaaccctt
     3781 gaaccaagca aaacagcgag ttcctcggtc acgcgcccca cgctgccagc accaatggtg
     3841 agaacagcat ctccggggcg cgcttcgcgt aaaatatatt ctatgccgcc ctgcaacgta
     3901 ctcgagtaaa caacactttt gtgtccggca gagctgattg cctctgcaag aagctcgccg
     3961 gtaatacctt ccgcaggcgg ttcgccggcg gcgtaaatgt ccgtcagaac aagaatgtca
     4021 gcctggttga aggaaaggcg gaaatcctcc caaagatgct gcgttcgcgt ataacggtgg
     4081 ggctgaaaaa gtacgagtag gcgcttgtag ccgcagcctc gcgcggcttc gagcgtagcg
     4141 cgaatttcag caggatggtg gccgtaatcg tcgatcagcg tgacgccgga aaagtcgcct
     4201 ttgatttcga acctgcggcc gacacctgaa aaatgtgtaa ggccttcacg aatcaaatcg
     4261 gccggaatgt ttagctcgag cgccgccgct gctgctgcgg cagcattacg gacattgtga
     4321 atcccgggcg gcgccggtag acgaaaaact cccaagtcct cgccgcgata tttcaacgag
     4381 aattggctcg aaagccctga gagcttcaca tccgaaataa ctagatcggc ctggctcgac
     4441 gtgccatagc tgatgatggg ccgcttgata cccgggacga ttgcttgggc gttgggttcg
     4501 tcaaggcaga gcaccacagc cccataaaac ggcacgcgat tggcgaactg aagaaacact
     4561 tcttgaattt cctcgagcga gctgtattgg tcaaggtgct ctcggtcgat cgtggtgatg
     4621 acggcgataa cgggcgctag aagaaggaac gagcggtcgc tctcatccgc ttccacgacc
     4681 atatattcgc ctcggccgac agctgcattg gctccaatgt gatttactcg gccgccaacg
     4741 acgaaggtcg ggtcaagatc cgctgcggcg agtatcgacg ccaccatgga ggtggtggtg
     4801 gtcttgccat gggcgcccgc cacggcgata ccgtatttca gccgcatcag ttcggcgagc
     4861 atctcggcgc gaggaatcac gggaatcttc aaccgatggg cctcggctac ttccacattg
     4921 tcaccgggca cggccgagga aacgacgagc acgtgcgcac catgggcgtt ttcggcctta
     4981 tgggtatcgt ggattaccgc ccccagtttc cgcaaacgct gggtggcagg cgaggatttc
     5041 aaatcagacc ccgaaacgcc atagccgctc gacagcagga cttccgcaat gccgctcatt
     5101 ccgctaccgc caattccgac cagatgaatg cgctgaaaat tgcgaaaagt gatgttcatg
     5161 gccgggccac cccgtcgatc acgttaacaa tatcttcaac cgcgcgcgga cgtcccagcg
     5221 aacgcgcgcg gtcttccatt tcttgaactc tctggggctg gtccaggagg aaaaaaattt
     5281 cattagttag gcgctctgga gtcagctcat cttgcggcag cagccgggag gcgccggcgc
     5341 ttgccatgac tcgtgcattt ccggtttggt gcgcgtcggt cgaagcaccg aagggaataa
     5401 agatggcggc acgtccggag gcggatacct ccgcgacagt gatggcaccc gaccggcaaa
     5461 cgatcaggtc ggcctgagca aacctctccg ccatattttc gatgaagggg acaacctcag
     5521 cttggaattg gcgacgatca tatgcttcgc gaaccgcatt atagtcacgt tcgccggtct
     5581 gatgcacgat gaaaaggccg tccttttttg tcgcgaggat atctagcgaa tcaacgacgg
     5641 ctcgattgat tggcgaagca ccgcggcttc ctccggtgaa cagtaacgtg aaaggtgggc
     5701 ggtggtcctt cgacggaatg gcgaagaact ccgggcgaat cgggcagccg gtgacggttg
     5761 ccctggagcc cagccgaccg gccgtttcct ggtgcgccac ggcaatcttg gtggcaatcg
     5821 ctgcaagaac gcggttggtg aatcccggct cgacattcgg ctcgaaaacc actgtaggta
     5881 cgcggtgcat cgccgccagc agcatcatcg gaccggaggc gtacccgccg actccgaaag
     5941 ctgcattgaa gcgatagcgg tgcagaatct tctctgaatc ccaaagcccg gcgggaacca
     6001 tcgctgcatt gcgcagcaac ttcacaccgc caatcccttt caggccgctc gcccgaatca
     6061 gttcgagcgg aaagcacgcc tggggcacca gtcgcgtttc gattcctcgc tcggttccta
     6121 caaacacgac ttgcctttct gactcacccc tgtcagcgcc acggcgaatc cattccttgg
     6181 cgatggcgag cgctggaaat acatgtccgc cggtgccacc gcccgcgatc aaaagtcttt
     6241 tcgcatcggc gctcacgtct catccgagtg ttggctgatg ttcagcaaca cccccgtggc
     6301 gagcagcatt cccatcaggc tcgacccgcc ataactaatg aagggaagcg ggatcccttt
     6361 tgtggggacc aggccgagca caacgctaag attgaagagc gcctggccga ctaccatggt
     6421 ggtaatgccg atacccaaga accgcccaaa ggcatcgggc gccttcatcg cagccacgaa
     6481 ccctcgccaa ccataaacgg caaaaagtgt gagaacgata attgcgccga taaaacccgc
     6541 ctcctcgcat atcaccgaga aaatgaaatc cgtgtgagcc tcagggagga aaaagagctt
     6601 ttggcggctc tccattaaac ccacaccgtt gaatcctccc gagccaacag caatcagcga
     6661 ttgtgccaac tgaaagccat gaccctgtgg atcggcaccc ggcgagaagt atgcctgcac
     6721 acgttgcagg cggtatggaa cactcgcaat ggcgatgtaa accaggggaa tagcggctat
     6781 tgctgcaccg aaaatatagc ggtatgacag tccggcgata aagagcatca cggccgcaat
     6841 gaagaaaatc atgcaggcag ttcccatgtc gggctctttg taaaccagcg cgacaatcag
     6901 aagcaccgcc cccaacacgg gcaagagagt ttgagtcagg ttgtttacgc ctggtccccg
     6961 cggttcgcgc cgaatttcca gaaaccacgc gaggtaaaaa attacgacca gcttggcaat
     7021 ttcggatggt tgcaaactga gaggcccgac gcgaacccag cgatgagtag catgggttcg
     7081 atccaagaag aacacggcga gtagcaaaat cagagtcacc gatacggccg tgaaaatcac
     7141 ccgcggctgg cgaagtttgc ggtaatcggt gttcatcaat agaaacattc ctgacattcc
     7201 aaacgtggcc caaaggagtt ggcggagaag aaattcgtag ccgttgccac cttgttcgcg
     7261 gagggtcact gccgatgcac tgaaaaccat gaccgcgccg atcaggcaca gtgccagagt
     7321 ggcaccgaac aattttcggt catgttgcaa gttacgaggc atttctatcc tttccgcgcg
     7381 aacacatttt cattggacgc ccttgcaaaa tcctggacga gctgcttaaa gacacgaccg
     7441 cgatgctcgt aattctcgaa ctggtcaaaa ctggaacacg caggcgcgag cagaaccaca
     7501 tcgcccggct gcgcctgctc tatcgccgct tggaccgccc gatcgagcgt gccagcttga
     7561 agtattggaa gcccaacacc caaatccgaa gcaatctttt ccgcagcaga accgattaaa
     7621 attactcgcc gggcccttcg tcgtagtggc tcttgcaacg ggatataagg cccgcctttg
     7681 tccttcccgc ccaaaattac gatgagcggt ccagcgaacg cctcaattgc cttcaacgcc
     7741 gcatccacat ttgtcgcctt tgaatcgttg taaaattcca ccgagttaac cttgccaacc
     7801 agctctaatc gatgttcgac tccgcgaaac gtcttcacgc ccacagcaat atccgccggg
     7861 gctgcaccgg cgaggtgcgc agcagcgcag gcagccatga cattttcgag attgtgctcg
     7921 ccacgcaagg aaatttcgtc tcgccggccg accgcaagtt ccatgccttc gttacgaatc
     7981 gtgatctggt ttccttgcaa gaacgctcct tgcgctagtc gcttgcgccg gctgaaccag
     8041 aagatgtgtg gaaccgcggg cgcgcgcttg gtcacttctg gatcgtccgc gttaagcacg
     8101 gcaaaatcgc ggtcgagttg attttcaaag aggcgcacct ttgcagttgc gtattcttcg
     8161 aatgatacgt gccggtcgag atggtctggg gtcagattca aaaggacgcc aatttccggg
     8221 cggaacgtct cgatcgtctc cagctgaaag ctgctgattt ccgcgactgt gatcgttgaa
     8281 tccgtggcgg attcaaccag cgataagagt ggcactccga tgtttccacc catcagcgtg
     8341 ctgatgcgac ctgtcctcaa gatgtgactg atgagtgacg ttgtcgtcgt cttcccgttc
     8401 gagcccgtta cagcgattag cttaccgcgc agaaaccgcc aggccagctc gatttcgctc
     8461 cacacgggga taccgaggga cgttgctaat ttgagcgcgg gcaggttcgg agagacgcct
     8521 ggacttacaa cgattaagtc ctgctcggca aacgtggacg gaaggtgggt gccgagctcc
     8581 agtttcacgc ccgcgtcgcg cagttgtggc gcagtctccg caaggctgct ctcggtcctc
     8641 atgtctgtag ccgtgaccac ggcgccacgc tcggcggcga aaagagacac cacgaggccc
     8701 gtgcgcgcta gtccaactac taggactcgc tttccttcga gatcgaaacc cggtcgcatc
     8761 tgtcctacct taatttcagc gtcgtgagtg aaaaaagtgc cagcacgagc gcaatgatcc
     8821 aaaatcggac gatgattttc gactcgcccc atcccaccag ctcaaaatga tggtgcagtg
     8881 gagacatccg aaacacgcgc ttgcctgtca gcttaaagga agccacctga attaccacgg
     8941 acagcaactc cactataaaa atgccgccga ttgagatcat caggatttcc tgcttcaaga
     9001 tgaccgcaac tgttccgata gcgccaccca atgccaagga acccacgtca cccatgaaca
     9061 tttctgcggg atgagcgtta taccaaagga atccgagcga agctccagcg agagatccgc
     9121 agaaaattgt aagctcggcg gcgtccggta ttctctgaat atcgagatat tctgcgaagc
     9181 gggagttgct ggaaagatac gtaagaacgg ttagcgcgac cgcagagatc agcacgcatc
     9241 cgatggccaa cccatcgagc ccgtcggtga gattgacccc gttggataaa ccagcaatca
     9301 ccaaaacaag gaacaagacg aacggcaggt acgctagcgg ccaaattaag tggctatcta
     9361 agagcgaacc aatcaccaga tcgggatgca gccctttcac gaacggcacg ctcaattgcg
     9421 tcgaatacac ccccttcgcc tgtagcccga tcaggacagc acccacgaag acgcaggtca
     9481 gaacctgcaa tgcaaacttt ccgcgtccag tcagtccccg gttctgcttg cggatgacct
     9541 tattgtaatc atccacaaat ccgatcgccc cgaaggctag cgtggccacc acggccagta
     9601 ccacataagg atttcgcaga tcagcccaga gtagggtggg aattacgatc gccgccagga
     9661 tcagcagtcc gcccatggta ggcgttcctg ctttcgcctg atgccgctgc ggcccttcct
     9721 cgcgaatgta ctgcttcact tgaagcgagc gcagccgctt aatgatccat ggcccaagca
     9781 gcagcgaaag caacagcgct gtcaagctcg ctgcggctgt gcgcaccgtg atatagcgga
     9841 atacgttcag cggactgaag tgccgccgaa gcatgaagaa gaaaaaataa tagagcattt
     9901 tagttgcggc cttcgcgacc cgtgtcaaca tgctgcagtc cggggttctg cgcggacggc
     9961 cgatggtgcg cgtttatggc ttccaggact ttctccatcc gcactcccct agagcccttc
    10021 aacagcacca agtctccgct cataacgaat tccgagagga acttcccggc ttcggtggaa
    10081 ttctgaaaga aggccgttct ctcttgtgga tgccccgctt gaattgccgc ggccacaaaa
    10141 tctgcggcgt gcccctggac gccgacaatc caatctatgt ttcgcaacgc ggccgccaga
    10201 cgtccgcatt ctttgtgtag ttcaggcgaa gaactaccca actccagcat ttctcccgag
    10261 gcaaggattc gacgtcgata ccctggtgtc tgcgcaagca gcgtgaccag cgcgctcagg
    10321 gcagtcgggc tcgagttata ggtgtcgttg atgacagaaa atccatcttc aaagcgaacc
    10381 acttctccgc gtttgtccgc tgctgtgagg ctgggaaaca cttccttggc ctcattcgta
    10441 ccaatgcccc aaacactggc tgctgctagg gccgccagcg cgttcatcac gttatgcctg
    10501 cctatcagcg gcaactgaag ccgaacgcgg cgccctcgag aaacaaattc aaactccgaa
    10561 ccctcgatac cgcgctcttt gattgcttcc gcctgaaatt ccgctccttc gcgcgtcccg
    10621 aatcggatca ccggtccttt cgcaatctcc gcgaaacgcg ccactcgctc gtcatccgaa
    10681 ttcagtacgg cagtcgcatt tggccacgga agattttcga tcagttcgcg ctcagccaga
    10741 gcaatttcct cgagcgacgg aaaaaactcc agatgctcta ctgcaacgcg cgtgacaaca
    10801 ccaacatccg ggcgacaaat tcgcgtgagc tccgcgagtt ctccggaatg cgacatcccc
    10861 aactccacaa ctgccgcacc gtgttcgtca ctgagcttta gcagcgtgag cgggaggcca
    10921 tattcgttgt tcaagttgcc ttgtgtcttc agtacgcggt accgagcacc taaaagtgcc
    10981 gccagaatct ccttcgtagt tgttttccca acggaacccg ccaccgcacc gattaagcgg
    11041 cccccccgag ccttgcgcca actttgacag gcgtgcctgg ctaagtcctg cagaccccgc
    11101 agcgtgtcgt ccacagcaaa cagtttgccg cggatctcgt ccgggtagtt gaggaagcgc
    11161 tctcgagcga ccaatgccgc catggcgccg cgctccaatg ccgaggccac gaaagcgtgc
    11221 ccatcgtgtc gcggtccgcg tatagccaca aacagttccc cagcacccac ggtccgagaa
    11281 tcgatggata cgccaccgat tatggccgac ggctcaatcc gctg
//
LOCUS       DATNDT010000250         4670 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2877416, whole genome shotgun sequence.
ACCESSION   DATNDT010000250 DATNDT010000000
VERSION     DATNDT010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2877416"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1214)
                     /locus_tag="VN788_08050"
     CDS             complement(<1..1214)
                     /locus_tag="VN788_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20517.1"
                     /translation="MKLHGFVAALAMTAAALAAAPAEEQRPPVTFYKDIAPILFQNCA
                     PCHRAGESGPFPLVTYQDARKHAHQIAAVTRIRYMPPWLPERGYGDFADDRRLTDRQI
                     HMIEQWATAGAPEGSVADSPPAPVFTPGWQLGPPDLIVQPVKPFTVPASGPDVFWNFI
                     LSPTIGRARYVKAIEIRPGKARIVHHANLLVDRSHSARRQEIHRGDGFPGMDLIIASD
                     TFDPDSHFLFWKPGSTPPVEPDGLAWRLDPGDDLVLNVHLQPSGRPEAVQPSVGLYFT
                     DKPQTKYPMLIQLEHDGALDIPAGDKDFVASDDFRLPLDVDVLAIYPHAHYLGKLLDG
                     FATLPDGTRKPLIRIPDWDLNWQGVYRYRTPVFLPKGTVISMRFHYDNSDANPRNPNR
                     PPKRVIGGNQAT"
     gene            complement(1211..1771)
                     /locus_tag="VN788_08055"
     CDS             complement(1211..1771)
                     /locus_tag="VN788_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20518.1"
                     /translation="MCSARLLAVLALAGVMVFARSPAHPLDLSGHAVDPFATPANARV
                     LVFVRTDCPISSRYAPELHRIAAEFHFRGVSFWLVYPDPSETPAAIAKHLSDYRYGWP
                     ALRDPEKILVKRARVTVAPEAAVFNSSGALVYHGRIDDRVVDFGKSRPSASRHDLEDA
                     IAATLEGKAVAEPVTRAIGCFLADLE"
     gene            1832..2257
                     /locus_tag="VN788_08060"
     CDS             1832..2257
                     /locus_tag="VN788_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HXU20519.1"
                     /translation="MPEKKTLRAAREDAKEGKAPTTQAGEFVREEIEHIREGKHGARS
                     AKQAIAIGLSKARRAGIKLPPPKKGRASEATRIQAERDLEKGVKRPNAKPSSKRSRAT
                     ETALKREGRSAASPKALSKQAKQAASARKRRGKVTGSKT"
     gene            complement(2264..2992)
                     /locus_tag="VN788_08065"
     CDS             complement(2264..2992)
                     /locus_tag="VN788_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HXU20520.1"
                     /translation="MKRIYIGTSGWHYEHWLGTFYPARLSASRMLSFYLEHFDTVELN
                     NSFYRLPSEKALDTWRESTPPNFLFAMKGSRFLTHMKKLKDPGPGLEKFFARADRLGE
                     KLGPVVFQLPPNWPLNIERLQTFLKALPRHHRYAFEFRNPSWNTPDVYALLRAHHVAY
                     CIFEIAGFQSPIEVTADFVYVRLHGPGGKYQGSYDDAILQRWADWIGSVQSSSVYFYF
                     DNDEAGYAPKNALRLKKILGVREL"
     gene            complement(2989..3438)
                     /locus_tag="VN788_08070"
     CDS             complement(2989..3438)
                     /locus_tag="VN788_08070"
                     /inference="COORDINATES: protein motif:HMM:NF014522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HXU20521.1"
                     /translation="MSSQDQTTKDQTVSEDVDRRLSTMNLLDTMSADPSLAEFLAAIR
                     ATGLQGNLGGPNLLTVLAPCNAAFEHIGRAIDPAMIGELARTHVLQGAWTAPELVRSG
                     SVKTLQGGTLEIHAEEATTRVSGVGIARADIECTNGVLHVLDGVLGV"
     gene            3529..>4670
                     /locus_tag="VN788_08075"
     CDS             3529..>4670
                     /locus_tag="VN788_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU20522.1"
                     /translation="MREKSSSLPPRAAFQYPNFRYYVAARFLTTSSSEMQAVAVAWQV
                     YALTHRPLDLGLVGLAQFLPGVFLFLIAGHAADRIARQRILQACYAAFSMCSILLLAF
                     TVHGLLSAYPIYLALLLNGVVRAFNGPAGQAFLPLLVAEEHFPNAVAWSSSIFQAATI
                     AGPIAGGLLYGFTGSPEVVYAYAGFAYLTALLLMSRIRVKSTERARPVEPPAFGLDGL
                     RYIWRNKLILGAISLDLFAVLLGGAVALLPVYAREILKIGATGLGILRSAPGVGAVIM
                     AVVVAHWPLRRRAGATMLWCVFGFGILTIVFGLSRNLALSLASLVLLGACDMVSVIVR
                     HTMIQLGTPNEMRGRVSAVNMIFIGTSNEVGQFESGITAQWFGTVPS"
BASE COUNT         1029 a         1273 c         1433 g          935 t
ORIGIN      
        1 tcagttgcct ggttaccgcc aatcacgcgc tttggtgggc gattgggatt acgcggatta
       61 gcatccgaat tgtcgtaatg gaagcgcatt gagatcactg tacctttcgg cagaaaaacc
      121 ggcgtacggt agcgatagac cccttgccag ttaagatccc aatcgggaat acggatcagc
      181 ggcttgcgcg tgccatcggg aagcgtggca aaaccatcga gaagttttcc gaggtagtgc
      241 gcgtgcgggt agatagccag tacgtcgacg tcgagcggca accggaaatc gtccgaagcg
      301 acaaaatcct tgtctccggc cggaatatca agagcgccgt catgttcgag ttgaatcagc
      361 atcgggtact ttgtttgcgg cttgtccgtg aaatagaggc caactgatgg ctgtaccgct
      421 tccggcctgc cagacggctg caagtgcacg ttcaggacaa gatcgtcgcc gggatcgaga
      481 cgccacgcaa ggccgtccgg ctcaacgggc ggcgtgctgc ctggcttcca gaacagaaaa
      541 tggctatcgg gatcaaacgt gtcgcttgcg atgatgaggt ccatacccgg aaagccatct
      601 ccacggtgga tctcctggcg gcgggccgaa tgcgaacggt cgaccagaag attggcgtga
      661 tgaacgatac gcgccttacc agggcgaatt tcgatcgcct tcacgtaacg cgccctgccg
      721 atcgtaggag aaagaatgaa gttccagaag acatcggggc cgctggcggg aacggtgaat
      781 ggtttgacag gctggacgat cagatccggc ggccccaact gccaacctgg cgtaaagacc
      841 ggagcaggag gagaatcggc aacagaaccc tcgggagcgc cggcggttgc ccattgctcg
      901 atcatgtgta tttgccgatc agttaggcgg cgatcgtccg cgaaatcacc gtagcctcgc
      961 tccggaagcc acggaggcat gtagcggatc ctggtgacgg ccgcaatctg atgcgcgtgc
     1021 tttcttgcgt cttggtaggt caccagcgga aatggtccgg actcgcccgc gcgatgacat
     1081 ggcgcgcagt tttggaacag aatcggcgca atgtctttat agaacgtgac cggcggccgc
     1141 tgctcttcgg ccggcgctgc ggccagagca gccgctgtca tcgcgagcgc cgcaacgaac
     1201 ccgtgcagct tcattccaga tcggcgagaa aacagccaat ggcacgagtc acgggttccg
     1261 ctacagcttt tccttcaagt gtcgcggcga tcgcgtcttc gagatcgtga cgggaagccg
     1321 atgggcggga ctttccgaaa tcgacgacgc gatcgtcaat ccggccgtga taaaccaaag
     1381 cgccggacga attgaacacg gcggcttcgg gagcaacggt cacgcgagcg cgcttcacca
     1441 gaattttttc tggatcgcgt agagcgggcc agccgtaccg ataatcgctg agatgcttcg
     1501 cgatggccgc cggggtttca ctggggtcgg ggtaaacaag ccagaaggag actccgcgga
     1561 aatggaattc cgccgcaatg cgatgaagct cgggggcgta gcggctggaa atcggacagt
     1621 cggtccggac gaagacgaga acacgggcgt tggctggagt ggcgaacgga tctactgcgt
     1681 gaccggaaag atcaagcggg tgggctggac tgcgcgcgaa aaccatcact cccgccagcg
     1741 caagcacggc aagcaggcgg gccgaacaca taaccgaaga atatcaccag cccacgtgga
     1801 aggcgcatac tgtaactgga ggagcgctaa tatgccggag aaaaagacgc tgagggcggc
     1861 gcgggaagat gccaaagaag ggaaagctcc gacgactcag gcgggcgaat tcgtccgcga
     1921 agagatagaa catattcggg agggcaagca cggagcccgt tcagccaagc aggccatagc
     1981 gatcggcctc tccaaggcgc ggcgtgccgg cataaagctc cccccgccga agaaagggcg
     2041 ggcatcggaa gctacgcgca tccaagccga acgcgacctg gagaaagggg tgaagaggcc
     2101 aaatgcgaag ccttcctcga agcgctcgcg ggcaaccgag accgccttga aacgagaggg
     2161 ccggtccgca gcttctccta aagcgctttc aaaacaagca aaacaggccg cgagtgcgcg
     2221 caagagacgc ggcaaagtga caggttccaa gacgtaacgc gaatcagagc tcgcggacgc
     2281 ccagaatctt tttcagccgg agtgcgttct taggagcgta accggcctcg tcgttgtcga
     2341 aataaaaata gacggaagag gattgaacgg agccgatcca atcggcccac cgctgaagga
     2401 ttgcgtcatc gtaactgcct tggtatttgc ctccggggcc gtgcaggcgg acataaacga
     2461 aatccgcggt gacttcgatc ggcgattgga acccggcgat ttcaaaaatg cagtaagcga
     2521 catggtgggc acgcaacaat gcgtacacgt cgggcgtatt ccaggacgga ttacggaact
     2581 cgaatgcgta acgatgatgc cgcggaagcg ccttcaagaa agtttgtagc cgctcaatgt
     2641 taagcggcca gttcggcggc agctgaaaaa caaccgggcc gagtttttcg cccaggcgat
     2701 cggcacgcgc gaagaatttt tccaacccag gaccgggatc cttcagtttc ttcatgtggg
     2761 ttagaaaccg gctgcctttc atcgcgaaaa ggaaattggg aggcgtagat tcgcgccagg
     2821 tgtccaatgc cttttcggag ggcagacgat agaaactgtt gttcaattcg acagtgtcga
     2881 aatgctccag ataaaaggag agcattcgtg aagccgacag ccgggcggga tagaatgttc
     2941 cgagccaatg ctcataatgc caacccgaag tgccaatgta gattcgcttc acacgcccaa
     3001 tacgccatcg agaacatgca ggaccccatt ggtgcactcg atgtcggctc gcgcgatgcc
     3061 aacgccggaa actctcgttg tggcttcttc agcatgaatt tcgagcgttc cgccttgcag
     3121 agtcttaact gaaccggaac gcaccaattc gggagcggtc caagctcctt gaagaacatg
     3181 agtgcgggcg agttctccga tcatggccgg atcgattgcg cgaccgatat gctcgaaagc
     3241 agcgttgcag ggagcaagta ccgtcagcag attgggcccc ccgaggttac cttgaaggcc
     3301 ggttgcccgg atggcggcca gaaattcagc gagtgatgga tccgccgaca tagtgtccag
     3361 aagattcatg gtggagaggc gccggtcgac atcttcggaa accgtttggt cttttgtggt
     3421 ctggtcctgg gaactcatac ctagaagact ccggtggatt gcaacgcgct acgctaattt
     3481 tcgaatcgaa gcctcttgac gagctatggg acactgacgc ttacgcgaat gcgtgaaaag
     3541 tcatccagct tgccgccccg cgccgctttt caatatccaa atttccgtta ttacgtcgcc
     3601 gcgagattcc tgactacgag ctcgtcggaa atgcaggccg tcgcggtagc ttggcaggta
     3661 tacgcgctga cacatcgccc gctggatctt ggattggtcg ggctggcgca gtttctacct
     3721 ggcgtgttcc tattcctaat cgcagggcac gcggcggacc ggatcgcgcg gcaacgtata
     3781 ttgcaggcct gctatgccgc tttctcaatg tgttccatct tgctgctggc gttcaccgtg
     3841 catggcctcc tttccgcgta tcccatctat ctggccctgc tgctcaatgg tgtcgtgcgg
     3901 gctttcaacg ggccggcggg acaggcgttt ctgccccttc tcgttgccga agaacatttc
     3961 ccgaatgccg tcgcctggag ttcttccatt tttcaggccg ctacaatcgc cggaccaata
     4021 gctggtggcc tgctgtacgg tttcactgga agtccggagg ttgtctatgc atatgcgggc
     4081 tttgcgtatc tcaccgcatt gcttctgatg tcccgaatcc gcgtgaagtc gacggaacgg
     4141 gcgcgtccag ttgagcctcc ggcattcggt ctggacggat tgcgttatat ctggcgcaac
     4201 aaactgattc tcggcgctat atccctcgac ctcttcgccg tactgctggg cggagcggtt
     4261 gccttgctac ccgtgtacgc gcgcgaaatt ttgaaaatcg gcgcgacggg acttggaatt
     4321 ctgcgcagcg ctccaggagt gggcgcggtg atcatggctg tcgtggtggc ccactggccg
     4381 ctgcgacgga gggccggagc cactatgctc tggtgcgttt tcggctttgg aatcctaacc
     4441 attgttttcg ggctctcacg caatttggcg ctgtcgctgg cctcgctggt actacttggg
     4501 gcttgtgata tggtgagcgt cattgtgcgg cacacaatga ttcaactcgg cactcccaac
     4561 gaaatgcgcg ggcgtgtgag tgcggtcaac atgatcttca ttggtacatc gaacgaggtg
     4621 gggcagttcg aatcgggaat caccgcgcag tggttcggga cggttccgtc
//
LOCUS       DATNDT010000251         4539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2592564, whole genome shotgun sequence.
ACCESSION   DATNDT010000251 DATNDT010000000
VERSION     DATNDT010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4539
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2592564"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..2906)
                     /locus_tag="VN788_08080"
     CDS             complement(57..2906)
                     /locus_tag="VN788_08080"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXU20523.1"
                     /translation="MVFRQPVGRTLIWRRTRVASVVFFWAYCASTLAFAERLPLKFYT
                     TADGLPHNTVNRIVRDSRGFLWICTADGLSRFDGYTFTNFGVEQGLPQGNINDLLESR
                     DGRYWVATAGGLARFDPDGAPASLSPNNPRETLRKQSDFTIIAFEGQGQRAHSVTVLR
                     QDKSGTIWCGTDRGLFRLDEVSGRPILRAVDVGIPLQFSEQAAVTDLMEDGDGSLWIA
                     TFAGLFHRSLDGKSTRATRADGLPDDLIQGLMRDHLGRLWAATRRAGFFQFSSGNAQR
                     PVVVLRSYDPRDVLPTSWVFQLLETSDHRFWIASNLGLVEFFPATTGAASFRLYTTAN
                     GLSYREVTALKEDASGNLWMGTNTSGLMKLARKGFVTYGKDEGVVSVNSIFEDGEGAL
                     CVKGALAAKSIVQGKSPYHVGFFFGRMKGERFGGFDVAAMANAPGWVTEHVTVRAHDG
                     EWWLGTGTGVLRFRTVKNFAAMASARPLAWYTRRDGLAGSQTFRLFEDSHGNIWASTI
                     DSDGNGFARWNRATGKWTNLADVAGLPALTQDLPRSFAEDRQGHVWIGFNGVLGRYAN
                     GRFLWFGRNNGLPPGAISNMLVDHAGRLWLASAQSGLILMDGVNGEHPVFRAYTTADG
                     ISGNHIEVITEDLYGRLYLGTGRGLDRFDPKTGRVKHFTAADGLAPGLFRAAYRDHSG
                     ALWFGMTGGLSKLIPAGDEIASPPPIVLSGVTVAGLNRIFPFGERSLVLPDLASDQNR
                     VQIDFVGLDFTPGEILRYQYRLQNGEWSAPSQQRAVSLAGLASGHYRFQVRAITSNDV
                     PSTEPATITFTVLAPVWQRWWFLMLVATCVAATAWAAYRYRMAQVLAIANLRTRIATD
                     LHDDIGSNLTKIAILSEVARQQLGHNEAEAVSDGDSLSAIARISRESVSSMSDIVWAI
                     NPQRDHLLDLVRRMRLHAEQVFTTRGIRLTFRAPS"
     gene            complement(3079..3591)
                     /locus_tag="VN788_08085"
     CDS             complement(3079..3591)
                     /locus_tag="VN788_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20524.1"
                     /translation="MGFLDSLFGKKCPACGAKGARVTPIETRCPNPACQYFDPSLGRQ
                     EQPAAQQPSQAAQSSVRGKSGFIPQEGVEIRYRNFQGQEKTFKAEKNSLRRRKNHILA
                     RVAPSGEYIALSRDRIQNLGEVDLALPQAMRSGAPWPSGRERQVLNYHKKHGSTSPLY
                     EQIRARFPDW"
     gene            complement(3740..4087)
                     /locus_tag="VN788_08090"
     CDS             complement(3740..4087)
                     /locus_tag="VN788_08090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20525.1"
                     /translation="MLSAIAAEVVGNSVRNVAILDYYLKHVDAEEQLRILDRWLAEEV
                     LSLSFGGHKKGHFRRITFAQLREMGLPSLAHRRRMIRNGKIESPFFVWQREKSTRAFK
                     GTAARLVRSANAS"
     gene            4229..>4539
                     /locus_tag="VN788_08095"
     CDS             4229..>4539
                     /locus_tag="VN788_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20526.1"
                     /translation="MPELVGLLKPTMNHGRSHDEVCLLTALALQPKDIQCALNGLLTS
                     RCRFWALAEYKNVVRITKEAGMRGAGFESAIQLSEIDVGQNTGAGATKWHANGCADQL
                     LT"
BASE COUNT          997 a         1356 c         1297 g          889 t
ORIGIN      
        1 ttaaaaatca ggaatacgtc gcgacgaatg tcggccctca gcctgatgtc ctgctcttag
       61 ctgggagcgc ggaaggtgag ccggattccc cgagtggtga acacctgctc ggcatgcagg
      121 cgcatacggc gcaccagatc cagcaaatga tctcgctgcg ggttgatagc ccacacgatg
      181 tcgctcatgg aagagaccga ttcccgcgag atacgtgcga tggccgacag cgaatcgcca
      241 tccgacacgg cttcggcttc gttatggcca agctgctgtc gcgcgacctc gctcaggatc
      301 gcgatcttgg tcaggttgga gccgatgtcg tcatgcaggt cggtcgcgat gcgggtacga
      361 agattggcaa tcgccaagac ctgggccatg cggtaacggt atgccgccca ggcggtggcg
      421 gcgacgcacg tagcaaccag catgaggaac caccaacgct gccaaacggg ggctagtacg
      481 gtgaacgtta tcgtcgccgg ttccgtgctc gggacgtcat tagaggtaat tgcccgcacc
      541 tgaaaccgat aatgtccaga agccaggccg gcgagactga ccgcgcgctg ctggctgggc
      601 gcgctccact cgccattctg cagccggtat tgatatcgca ggatctctcc gggagtaaaa
      661 tcgagtccca cgaaatcgat ctgcacgcga ttttgatcag aggcgaggtc cggcagaacg
      721 aggctgcgtt ccccaaatgg gaatatcctg ttgagaccag ccacggttac gccgctcaga
      781 acaattggcg gcggcgatgc gatttcatcg ccggcaggaa tgagcttcga caatccgcca
      841 gtcattccga accacagcgc cccactatgg tcgcggtagg cagcccggaa aagaccggga
      901 gccaggccat cggcggcggt gaagtgcttc actctgccgg ttttaggatc gaaccggtcg
      961 agtccgcgcc cggtcccaag gtatagccgg ccatagagat cctcagtgat gacctcaatg
     1021 tggttgccgg agatgccgtc ggcggtcgtg taagcccgga aaactgggtg ctcgccattt
     1081 acaccgtcca tgagaatcag gccactttgc gcggacgcga gccaaagccg cccggcgtgg
     1141 tccacaagca tgttcgagat ggcgccaggc gggaggccgt tgtttctccc gaaccagagg
     1201 aagcgtccat tggcgtatcg acccagcacg ccattgaagc cgatccatac gtgaccctgc
     1261 cggtcctccg caaacgaccg cggcagatcc tgagtaagcg ccggaaggcc ggcaacatcg
     1321 gccaggttcg tccatttgcc ggtcgcgcga ttccaccggg caaaaccatt tccatccgaa
     1381 tcgatggtgg aggcccagat atttccatgc gagtcttcga agagacgaaa tgtttgcgat
     1441 cccgcaagac cgtctcgtct ggtgtaccag gcaagcgggc gcgccgaggc catggcggcg
     1501 aaatttttca cggtgcggaa acggagaacg cctgtgccgg tcccgagcca ccattcgccg
     1561 tcgtgggcgc gcactgtcac atgctccgtc acccaccctg gcgcatttgc cattgccgct
     1621 acgtcaaagc cgccaaagcg ttctcctttc atccggccaa agaaaaaccc gacgtggtat
     1681 gggctttttc cctggactat gcttttggct gccagcgctc ctttcacgca aagcgcacct
     1741 tcgccatcct cgaaaatcga gttaacgctc accacgcctt catctttccc ataggtgaca
     1801 aagcccttgc gcgccagctt catcagtcct gaagtgttgg tgcccatcca gagattgccg
     1861 ctcgcgtcct ccttcaaggc tgtgacttcg cgatagctca atccattcgc cgtggtatag
     1921 agcctgaagc tcgcagcgcc ggttgtggcg gggaagaatt ccaccagtcc caggttgctt
     1981 gcgatccaga atcgatggtc cgaggtttcg agcaactgaa agacccacga ggttggcagt
     2041 acgtcgcgag ggtcatagct tcgcagaacc acgaccggtc gctgcgcatt gccagaggag
     2101 aactggaaga agccggcgcg gcgtgtggcc gcccacaaac ggcccagatg atcacgcatg
     2161 aggccttgga ttaggtcatc cggcaagcca tcggcccgcg tagctcgtgt gctcttgccg
     2221 tcgagagaac ggtgaaacaa tccggcgaaa gtagcgatcc ataaggaacc atcgccatct
     2281 tccatcagat cggtgacggc agcctgctcg gaaaattgaa gcggaattcc gacatccacc
     2341 gcgcgcagaa tgggccggcc gctaacctca tcgaggcgaa acaatccccg atcagtacca
     2401 caccagattg tcccgctctt gtcctgccgc agaactgtga cgctgtgagc gcgctgcccc
     2461 tgaccttcga atgcaatgat cgtgaaatca gactgtttcc gcagggtttc gcgcggattg
     2521 tttggagaaa gactggccgg ggcgccatca ggatcgaagc gcgcgagacc tccagccgta
     2581 gctacccaat atcgcccatc gcggctctcc aaaaggtcat taatattgcc ttgcggcagc
     2641 ccctgttcta caccgaaatt cgtgaacgta tagccgtcga aacgggaaag gccgtcggcg
     2701 gtgcagatcc acaaaaaacc gcgcgagtca cgcacaatgc gatttacggt gttgtgcggc
     2761 aagccatcag cagtggtgta aaacttcagc ggcaaccgtt ccgcaaatgc cagcgtggaa
     2821 gcgcaatatg cccagaaaaa cacaacggat gcgacgcggg tacgtcgcca tatcagtgtc
     2881 ctgccaacgg gttgtctaaa aaccacggcg taactcccgg ccctgaggag tcgaaacact
     2941 atacgcctga gaaagccgaa agagtgcaac ggaaatccca tgccgcggct gaaatccgca
     3001 gcccagggat tgtggcggtt cgctcaagcg ctgctgcttt taccgggaaa cgcggcgagc
     3061 ccgtcagcaa agcatgaact accagtccgg aaacctggcg cggatctgct cgtagagtgg
     3121 ggaagtgctc ccatgttttt tgtgatagtt gagcacttgg cgttcccgtc ctgaaggcca
     3181 gggtgccccc gaacgcatgg cttgggggag ggcaagatcg acttcgccca ggttctgaat
     3241 gcggtcgcgg gagagcgcaa tgtattctcc gctgggcgcc acgcgcgcga gtatgtgatt
     3301 ttttctgcgc cgcaatgagt tcttctcggc tttgaaggtt ttttcctgcc cttgaaaatt
     3361 gcggtaacga atctccacgc cctcttgcgg tatgaagcct gactttccgc gcaccgaact
     3421 ttgcgctgct tgtgaaggtt gctgagcggc aggctgttcc tgccttccca gactgggatc
     3481 gaaatattgg cacgccgggt tcggacagcg agtttcaatc ggggtgacgc gggcgccctt
     3541 ggcgccgcaa gccgggcatt ttttgccaaa gagactgtcg agaaatccca tgattagctc
     3601 cgcgatcaga cgctcaaggg gacggctgga aaaaacgaac caaggcttta gcctctggtt
     3661 attccataac acccatctac aggcggccac cctctctcac gggcacttgc agccgctgct
     3721 tctgggcctg aagagaagat cagctcgcgt ttgcgctgcg aaccaatctg gcagccgtcc
     3781 ccttgaacgc ccgagtagac ttttcccgct gccacacgaa aaacgggctt tcaatttttc
     3841 cattgcgaat cattcgacgg cgatgcgcca gagacggcag ccccatctcc cgcaactgcg
     3901 cgaaggtgat tcggcgaaag tgccccttct tatgacctcc gaacgacaga ctcaggacct
     3961 cttccgccag ccagcggtcg agaatccgca gttgctcctc ggcgtcaacg tgcttgagat
     4021 aataatcgag aatcgccaca ttacgaacgc tgttgccgac gacctccgcg gcaattgccg
     4081 acagcaatcg ggcgcgctcc tcaggaactc gtaccggctt ccattgcctg cgtctacgac
     4141 gaaatgcgaa acgaaacagg ttctggatct tacgcgtttt atcgcgcgac agcgccacgc
     4201 ctccatccgc tcgaaataac aagccaagat gccggaactc gtgggcctgc tcaaacccac
     4261 tatgaatcac ggacgttctc atgacgaagt ttgcctgctg actgctcttg ctctgcagcc
     4321 caaggacatt caatgtgcac tgaatggttt gctcacaagc cgttgccgat tctgggcttt
     4381 ggcagagtac aagaacgtcg tccgcataac gaaagaagcg ggaatgcggg gcgcaggctt
     4441 cgagagcgcg atccaactga gtgagataga tgttggccag aacacaggcg ctggcgcaac
     4501 caaatggcac gccaacggtt gcgcggacca gctcctcac
//
LOCUS       DATNDT010000252         2260 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3763799, whole genome shotgun sequence.
ACCESSION   DATNDT010000252 DATNDT010000000
VERSION     DATNDT010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763799"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1046)
                     /locus_tag="VN788_08100"
     CDS             complement(<1..1046)
                     /locus_tag="VN788_08100"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HXU20527.1"
                     /translation="MNSGQEKRAAKHIHMLGVAGSAMAPVAGMLKERGFRVTGSDVNV
                     YPPASTLLDSLGIAWNEGYRAENLMPAPDLAVIGNAISRGNPEVERILDEKIPYCSMP
                     RIIEDYFLPGRDSIVVAGTHGKTTTTAMLAWIFQTAGRQPDFLIGGVAPNFGDRSYGL
                     GGGKEFIIEGDEYDTAFFDKGPKFLHYHPDELILTSLEYDHADIYPDLAAIALQFRRL
                     VNLVPRRGRILICGGSRELKEVASKAFCPVETFGLAAECDWQASDIVWDETATKFRVI
                     FRGSEVARIRIPVAGTHNVLDALAAIALAHGRGVDRDAIAQALATFRSVRRRMEIKGE
                     AGGVLVVEDFAHHPT"
     gene            complement(1080..2111)
                     /locus_tag="VN788_08105"
     CDS             complement(1080..2111)
                     /locus_tag="VN788_08105"
                     /inference="COORDINATES: protein motif:HMM:NF014114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HXU20528.1"
                     /translation="MSTAARNSYLLESFVDVDAFAEIPYSRLTDMQPSHKPHALRPGD
                     VVRVLSLASPVQEDRLQKGYDEIKRLGYTPQMEAPVPRQEGSFFADSAENRLRALQSA
                     LQDSGARAIFCSRGGYGSNYLIEGLGAAAAATAPKIFLGSSDITSLQAYLWEKLNWVT
                     FYGPMVASNFDQGAGAGNGYDEASLIRALTVTEDGWMLDLKGEPLIKGSARGITVGGC
                     LTLLETTLGTPWEIETNGAILVLEDRGMKPYQVDRALMHMKQAGKFRAVEGIVLGEFP
                     DCEGPVGDETVRDTVRRVLGDSGIPIVWGVPVGHTSRPMLTVPLGVLAELSSAGSGTL
                     RILEPACTA"
BASE COUNT          482 a          735 c          590 g          453 t
ORIGIN      
        1 gtaggatggt gcgcgaaatc ctcgaccacc agcacgccgc ccgcttcccc tttgatctcc
       61 attcgccggc gcacgcttcg gaacgtcgca agcgcctgcg caatcgcatc gcgatcgacg
      121 ccccggccgt gcgccagcgc aatcgcagca agcgcgtcca acacattgtg agtcccggcc
      181 acggggatcc gaatgcgtgc cacttcgctt ccgcgaaata tcactcggaa tttcgtcgct
      241 gtttcgtccc aaacgatatc gctggcctgc cagtcgcatt ccgccgccag cccaaaagtc
      301 tccaccgggc aaaacgcctt ggaggccact tccttgagct cgcggcttcc gccgcagatc
      361 aaaattcgcc cacggcgcgg aaccagattc accagcctgc ggaactgcag cgcgatcgct
      421 gccagatccg gataaatatc cgcgtgatca tattcaagcg acgtgaggat cagctcgtcg
      481 gggtggtaat ggaggaattt cggtcctttg tcgaaaaaag ccgtatcgta ttcgtcccct
      541 tcgatgatga attcttttcc acctcccaga ccgtagctgc ggtcgccaaa attcggggcc
      601 acgccgccga tcagaaagtc gggttgccgg cctgcggtct gaaaaatcca ggcaagcatc
      661 gccgtggtcg tggttttgcc atgcgttccg gcgacgacaa tcgagtcgcg ccccggcaaa
      721 aagtagtcct cgataattcg tggcatcgag caatagggaa ttttctcgtc aaggatgcgt
      781 tccacctcag gattgccgcg cgagatggcg ttgccgatca ccgcgagatc cggcgcgggc
      841 atcagatttt ccgcgcggta tccttcgttc cacgcgatgc ccagtgaatc gagcagtgtg
      901 gacgccggag gatacacatt cacgtccgag ccggttacgc gaaagccgcg ttccttcagc
      961 atccccgcca ctggagccat cgccgatcca gcaacgccca gcatgtggat gtgtttcgcg
     1021 gctcgctttt cctgaccaga attcattcgc tgcgccctct caaggtcgcc gaacatattt
     1081 tatgcggtgc aagcgggttc caaaatacga agcgtcccgc tgcccgcgct cgatagctcg
     1141 gccagcactc ccaaaggaac cgtcaacatc ggacgcgagg tatgtcccac cggcacgccc
     1201 cagacaattg gaatcccgga atcgcccaac accctgcgca ccgtgtcacg aacagtttcg
     1261 tcgcccacgg gcccttcgca atccgggaac tcgcccaaca caattccttc cacggctcta
     1321 aactttccgg cctgcttcat gtgcatcagc gcgcggtcaa cctgataagg tttcatcccg
     1381 cgatcttcga gcaccaaaat tgcgccgttc gtctcgattt cccacggcgt gccgagagtc
     1441 gtctcaagca gcgtcaggca gccgccgact gtaattcctc gcgccgaccc tttgatcaac
     1501 ggctcgcctt tcaaatcgag catccagcca tcctcggtaa ccgttagcgc gcgaatcagg
     1561 ctcgcttcat cgtaaccatt ccccgctccc gcgccttgat cgaagttcga cgcgaccatc
     1621 gggccataaa aggtcaccca attcagtttt tcccacaagt acgcctgcag cgatgtgata
     1681 tcgctcgatc caagaaaaat cttcggagcc gtcgccgccg ccgccgcgcc gaggccttca
     1741 atcaaatagt tggaaccgta accgccgcgc gaacaaaaaa ttgctcgtgc gcccgaatct
     1801 tgcagcgcgc tttgcaaagc gcggagacga ttctccgctg aatctgcaaa aaaggaccct
     1861 tcttgccgcg gaaccggagc ctccatttgt ggcgtatagc ccagccgctt aatttcgtcg
     1921 tagcccttct gcaagcgatc ttcctgcacc ggactggcta gcgaaagcac gcggaccacg
     1981 tcacctgggc gaagcgcatg cggcttgtgc gacggctgca tatcggtcaa tcgagaatag
     2041 ggaatttccg cgaacgcgtc tacatccaca aaggactcga gcaggtaaga gttacgagcc
     2101 gcggtgctca attgactgta aatgtcagag ttttggaaag accgccgccc ggcgccggcg
     2161 ttgttactgc cacctgtaac atacccttcg ccgcaagatc cgcggccgga atcactgcga
     2221 tgagttgcga ggcgctcaag aaagaagtag ctagcggtac
//
LOCUS       DATNDT010000253         3937 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2014697, whole genome shotgun sequence.
ACCESSION   DATNDT010000253 DATNDT010000000
VERSION     DATNDT010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3937)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3937)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3937
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2014697"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2111)
                     /locus_tag="VN788_08110"
     CDS             complement(<1..2111)
                     /locus_tag="VN788_08110"
                     /inference="COORDINATES: protein motif:HMM:NF037626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HXU20529.1"
                     /translation="MILPLVSELIARIARLEAIRRGCEKVRRKEAEVAFAGFTDSAKS
                     ILLPLIFRELGRPVIFLVDSNQRAEEILEPLRWFYRAIASKPGSRVVCFPAYEVLPYE
                     NRSPHAEISEARAVALWRLASGEADLVVAPVQAALWRMRDRDFYRDLARLIARDDSIP
                     HEDLIGFLNRAGYEKQVTCETPGQYSVRGGIIDIFSPEAPQPVRVELLGDTIESIRAF
                     DPNTQRSTNPIERAALLPLTELLRGSEVLAHQAVASASGRVDETAPPDFYPGWEFREI
                     PLEHRPGILFDLPAEPLLAIDEPSLLQAAIEKYRARMQAAFEKAESPLTEPPNRYIFN
                     EDEWSLALQLFPQLSVEHLALTGNGSETFALQTQPTTRHHGNVPAFMDEVRSRLQAGE
                     HVLVSAASTGELERFADICHEYDLPYSVGELEENVTVTRLAQESSASSAPAMVLVKAP
                     LAEGVVFPEARIAFYGNADLFETPPQLQRSRPKTASFFSDFSDLKPGDYVVHVDHGIG
                     QFEGLRQVIVEGATGEFMLLKYAGDAKLYVPLARLDLIQKYQSLGGVEPALDRLGATL
                     WEARKTRVRKSVNDMAEQLLALYAERKTAPGHAFPKDSAWQREFEDAFEFEETTDQAR
                     AIDDVKRDMESPLPMDRLLCGDVGYGKTEVAMRAAFKALADSKQVAVLAPTTVLAFQH
                     LETFRRRFAAFPARIEMLSRFR"
     gene            2557..3747
                     /locus_tag="VN788_08115"
     CDS             2557..3747
                     /locus_tag="VN788_08115"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXU20530.1"
                     /translation="MKDIAIVGGGPAGALCGERLASHGFNVTIFDERLAWEKPCGGGL
                     THKAIQAYPFLLEGSHPKKIVRTAELISGGGDRARFEMSHPIVIYARKVLNGLLLDRA
                     IAAGCTAVRSRVTSVDTEGTQVYLTVGGETRRSDFVVLAAGARNQLLPGTTALGTKDL
                     EVTLGYFVPTEEDILKVKFLKEFEGYLWSFPRADHLSVGICAKMGQSPAQQLRRHLDQ
                     FVDEEKIPMAGARFYSHMLPSPQAQTIRNRRIVGRNWAMAGDAAACVDPVTGEGLYYA
                     LRSGDLLAQALADGQPQAYPERLRAAFSADLELAAQIARKIFRGNFLGGAITTRMVQL
                     LNYSPAFRDLIRDVFSGTQDYRTLKQRLWDQFGITLVEFIRSWFGFRGLAGATDGVGR
                     EYLP"
     gene            3744..>3937
                     /locus_tag="VN788_08120"
     CDS             3744..>3937
                     /locus_tag="VN788_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20531.1"
                     /translation="MRKPKSKRGKRRASSAAKWQKRSSAAAPTHRDGPRLGLEATIER
                     VVRPEHTLQTIDPNLPAVFST"
BASE COUNT          811 a         1027 c         1340 g          759 t
ORIGIN      
        1 cgaaagcggc tgagcatttc gatgcgcgcg ggaaaagcag cgaagcggcg gcgaaatgtt
       61 tccagatgtt gaaaggctag aacggtggtg ggcgcgagga cggcgacctg ctttgagtcc
      121 gcaagagctt tgaaagcggc gcgcatggcg acttccgtct tgccgtaacc tacgtcgccg
      181 caaaggaggc ggtccatggg cagaggggat tccatgtcgc gcttcacgtc gtcaatggcg
      241 cgcgcctggt cggtggtttc ctcaaattcg aaggcatcct cgaattcgcg ctgccaagcg
      301 gaatctttcg gaaaggcatg gcccggagcg gttttgcgct cggcgtagag cgcgagaagc
      361 tgctcggcca tgtcgttcac ggatttgcgg acgcgggtct tgcgagcttc ccagagggtg
      421 gcgccgagac gatcgagcgc gggttccacg ccgcctagcg actgatattt ctgaataagg
      481 tcaaggcgcg cgagcgggac gtagagcttt gcgtcaccgg cgtatttcaa gagcatgaat
      541 tcgccggtgg cgccttcgac gatcacctgg cgcaagcctt cgaactggcc gatgccgtgg
      601 tccacgtgaa cgacgtaatc acctggcttg agatcggaaa aatcgctgaa aaagctggca
      661 gtcttgggcc gcgagcgctg caactgcggc ggcgtctcaa ataaatcagc gttgccgtag
      721 aaagcgatgc gggcttcggg gaaaacgact ccttcagcga gcggcgcttt cacgagaacc
      781 attgctggag cgctgctggc gctggattcc tgcgcgaggc gcgtgacggt gacattttcc
      841 tcaagctcac ctacgctgta gggcagatcg tactcgtggc aaatatcagc gaagcgctcg
      901 agctcgccag tgctggccgc ggagacgagc acatgttcac ccgcttgcaa gcgggagcga
      961 acctcgtcca tgaaggcggg gacattgccg tggtggcgcg tggtgggctg ggtttggagc
     1021 gcaaatgttt cagagccgtt gccggtgagc gctaaatgtt cgacggagag ctgaggaaaa
     1081 agttgaagcg ccaacgacca ttcgtcttcg ttaaagatgt aacggttggg cggttcagtg
     1141 agcgggcttt cggctttttc gaaggctgcc tgcatgcgcg cgcggtattt ttcgattgca
     1201 gcctggagga gggagggttc gtcgatggcc aaaagcggct cggcaggaag atcgaataga
     1261 attcccgggc gatgctcgag cggaatttca cgaaattccc agccaggata aaagtcggga
     1321 ggagcggttt cgtccacgcg ccccgatgcg gaggcgacag cctgatgcgc gaggacttcc
     1381 gagccgcgaa gaagctccgt gagcggcaaa agcgcggcgc gctcgatcgg attcgtggaa
     1441 cgctgcgtat tgggatcgaa cgcgcggatg gattcgatgg tgtcaccaag aagctcgacg
     1501 cgaacgggct gcggcgcttc gggcgaaaaa atgtcgatga tgccgccgcg aacggaatat
     1561 tgccccggcg tctcgcaggt gacttgcttt tcgtaaccgg cgcgattgag gaagccaatg
     1621 agatcttcgt gcgggatgga atcgtcgcgc gcaattaggc gagccaaatc gcgatagaag
     1681 tcgcggtcgc gcatgcgcca caaagcggcc tggactggag cgaccaccag atcggcttcg
     1741 cccgatgcga ggcgccagag ggcgacggcg cgcgcttcgg aaatttccgc gtggggcgag
     1801 cgattttcgt agggtagaac ttcgtaggcg ggaaaacata ctacgcgact gccgggtttc
     1861 gaggcgatgg cgcggtaaaa ccagcgcaac ggctcgagga tttcctcggc gcgctgattg
     1921 gagtcgacga gaaaaatgac ggggcggccg agttcgcgaa aaatcagcgg aagcaggatt
     1981 gattttgcgg aatcggtgaa accggcgaaa gctacttcgg cttccttgcg gcgaactttt
     2041 tcgcaacctc gccgaatggc ttcgaggcgc gcgattcgcg ctataagttc ggaaacgagc
     2101 gggagaatca tgggcggatt tcaccggcag cgcggataag gagcgtgaaa tgatgaggcg
     2161 aaagcggatg cgggcgatta tacgagcggg gcgggcacac tacccccttg ccgttgcgtt
     2221 tcgaggcgcc ccggtggaga agcaaatccc tttgcgaaac tgcgagtccc aatcgcttgc
     2281 ctatcggtcc gatcccggag gagcggccga agaaataatt ctatcacgct cgtttgaagc
     2341 gaaacgcgct gatgggtgaa cgcgaatact tgcggctgtg aatcaccacg gcgtgcctgg
     2401 tgcgaatgcg ttactccgcg cgacggctcg gcggctgccg gcgctctttt tggtgctata
     2461 ctcggcgcct agttcatcag ggtggtcgtt ctaattgcgc gccgcttacg tgattcacgc
     2521 acaacgggac cgggaaaagg gccaaactcg ccggccatga aggatattgc gattgtcgga
     2581 ggagggccgg ccggtgcgct gtgcggcgag cgcctggcaa gccacggatt taacgtcacg
     2641 attttcgacg aacgcctggc atgggaaaaa ccgtgcggcg gcgggctgac tcacaaggcc
     2701 attcaagcgt atccattcct gctggaaggc tctcacccca aaaaaatcgt acggacggcg
     2761 gagctgatct cgggcggcgg cgatcgcgcg cgattcgaga tgagccatcc catcgtgatc
     2821 tacgcgagaa aggtgctgaa cgggctgctg ctcgatcgcg cgattgccgc aggttgtacg
     2881 gcggtgcgct cgcgcgtgac gagcgtggat acggagggga cgcaggtata tttgacggtt
     2941 ggcggcgaaa cgcggcgctc ggattttgtg gtgctggctg cgggcgcgcg gaatcaactg
     3001 ctgccgggaa cgacagcgct gggtactaag gatctcgaag tgacccttgg ttactttgtt
     3061 cccaccgaag aagacattct gaaagtaaaa ttcctgaaag agttcgaggg atatttgtgg
     3121 tcgtttcccc gcgccgacca tctttccgtg ggcatttgcg cgaagatggg gcagagtccg
     3181 gcgcagcaac tgcggcggca cctggatcaa tttgtggacg aagaaaaaat tcccatggcg
     3241 ggcgcgcgct tttacagcca catgctgccg tcaccgcagg cgcaaacgat tcgcaatcgc
     3301 cgaatcgtgg gaagaaattg ggcgatggcg ggcgacgcgg cggcttgcgt cgatccggtg
     3361 accggcgagg ggctttacta cgcgctgcgg tcgggggatt tgctggcgca ggctctggcg
     3421 gatgggcagc cgcaagcgta tccggagcgg ttgcgcgcgg cgttttccgc ggatttggaa
     3481 ttggcggcgc aaattgcgcg gaagattttt cgaggaaatt ttctgggcgg cgcgatcact
     3541 accaggatgg tgcaactgct gaattattcg cctgcgtttc gcgatttaat tcgcgatgtg
     3601 ttcagcggca cacaggacta tcggacgctg aagcaacggc tgtgggacca atttggaatc
     3661 acgctggtcg aattcattcg cagctggttt gggtttcgcg ggctggcagg cgcgacggat
     3721 ggcgtgggac gcgagtacct gccatgagga aaccgaagag caagcgcggc aagcgccgag
     3781 cgagtagcgc ggcgaaatgg caaaagcgtt cgtccgctgc cgcaccgacg catcgcgacg
     3841 ggcctcggct cgggcttgag gctacgatcg aacgagtggt gcgtccggag cacacgctgc
     3901 agacaattga cccgaacctg ccggcagtat tttcgac
//
LOCUS       DATNDT010000254         3151 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2932542, whole genome shotgun sequence.
ACCESSION   DATNDT010000254 DATNDT010000000
VERSION     DATNDT010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2932542"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VN788_08125"
     CDS             <1..582
                     /locus_tag="VN788_08125"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXU20532.1"
                     /translation="PRWLVKIVAVMGGGGLFVATFLDSSVITLPFIADALVIRLSAQR
                     PARMPYYCAMAALGSLAGCIWIYLLAKKGGEAYFHRHGGRGSHKVKRWVQSHAFLSVF
                     IPGILPPPFPFKAFVLAEGVFQVPLRTFVLALLLGRGLRYFIEGILAVRYGDAVLLFL
                     TDHKIGFAISVVGIIVVLFVISRLVFPKAPATP"
     gene            complement(603..1631)
                     /locus_tag="VN788_08130"
     CDS             complement(603..1631)
                     /locus_tag="VN788_08130"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXU20533.1"
                     /translation="MEPVTHALTSLAFARAGQRRLPRFGMAMLIVAGIAPDLDYLSYF
                     GGGHTFIRFHRTVLHSLAGSALMACVLAGAFCALDRKYPRGAVTEKSFSPLHFREALA
                     VCAIGAGGHLLLDLASGVGVQVLWPFKTHWSAWDLVTNFDLWILLLLAAGLLLPLLFK
                     LVNEEVGEHKRRTGKWGGIVTLLLLAGYLGMKADLHSEAIELLLTREYHGRIPLSAGA
                     FPEGSAPLDWRGVLETDNTLETIDVQVGLNQPFDSNSSITHYKPQDSPALELGQKSSA
                     TAQFLEYARFPVAKVQRIEADYRFEVRDLRFEADDNEPANVLLRIDYDSRLQTRRAEF
                     RFASSTGQ"
     gene            complement(1650..2588)
                     /locus_tag="VN788_08135"
     CDS             complement(1650..2588)
                     /locus_tag="VN788_08135"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXU20534.1"
                     /translation="MPHNIKDALLIYNPASGRRRHRRSAHVEQAARILKDAGIATELA
                     ATTAAGSATEVARKAAEQRRGMVIACGGDGTINEIVNGLAGSDVPMALLPAGTANILA
                     KELGIPWDIRRAARLIHGGTVRRIALGAAFPLNGNHSALLSSKGRFFLSVAGAGPDGA
                     IVKGVHPGLKKNAGIAAYWFEGFRQLFQYDFPEIRIRSAGRERKATIIVVGRTVHYGG
                     PFKITTGASLFEDSFEVVTNSRRSRMGYVACLPAMVFGSLRAMDGIEAWKSAETICES
                     AGGEPIYAQVDGEPVGPLPVAFRIVPDALSIVTPGR"
     gene            2650..>3151
                     /locus_tag="VN788_08140"
     CDS             2650..>3151
                     /locus_tag="VN788_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HXU20535.1"
                     /translation="MSKAATIKISSAAKEIEELREKIRHHEYLYYVLDDPKISDATFD
                     RLMEQLKKLEGENPNLVTPDSPTQRVGGAPREGFRKVRHKTPMMSLDNAFSFEALAEF
                     DRRVRQTTGREKVEYACEQKFDGLSLSLIYEKGKLVAGVTRGDGTTGEDVTPNVRTIR
                     SIPLGID"
BASE COUNT          670 a          953 c          863 g          665 t
ORIGIN      
        1 ccacgctggt tggtgaagat cgttgccgtg atgggcggcg gcggattgtt cgtcgctact
       61 tttctcgatt cgtcggtaat cactcttcca tttatcgccg acgctctggt gatccggctg
      121 tcggcgcaac ggccggcgcg gatgccatat tattgtgcga tggcggcttt gggttcgctt
      181 gcaggctgca tatggatcta tctgctggcg aaaaaaggtg gcgaagccta ctttcaccgc
      241 catggcggtc gtggctcgca caaagtaaag cgctgggtgc aaagccacgc ctttctcagc
      301 gtattcattc ccgggatcct cccgccgccg tttcccttta aggcttttgt gcttgccgaa
      361 ggagtcttcc aggtcccgtt gagaacgttt gttttggcgc tcctgcttgg tcgcgggctg
      421 cgctatttta tcgaaggcat tcttgcagtg cgctacggcg acgcggttct actgtttctc
      481 accgatcaca agatcggttt cgctatttcg gtcgtgggaa tcattgttgt tttgttcgtg
      541 atcagccggc tggtattccc gaaagcgccg gcaacaccct gaaccgcccg gcaaacgagc
      601 cgtcactgcc ccgtcgacga agcgaaacgg aattctgccc gccgggtttg cagacggcta
      661 tcgtaatcga tccgaagtag cacgtttgcg ggctcgttgt catccgcctc aaagcgaaga
      721 tcgcgcacct cgaagcgata atccgcttca atgcgctgaa ccttcgcgac ggggaaccgc
      781 gcgtattcca gaaattgagc tgtcgctgaa cttttctgtc ccagctccag tgccggcgaa
      841 tcctgtggct tatagtgggt gatgctgctg ttggaatcga agggctgatt caggcctact
      901 tggacgtcga tggtttccag cgtgttgtcc gtctctagta cgccccgcca atcaagaggc
      961 gccgagcctt ccggaaatgc tcctgctgaa agaggaatac gcccgtggta ctcgcgtgtc
     1021 agcaaaagtt cgattgcctc gctgtgcaga tccgccttca ttcccaaata cccggcaagc
     1081 agtagcagtg taacgattcc cccccacttt ccagtccttc ttttatgctc gcccacctct
     1141 tcattcacca gcttaaagag tagcggcagc aaaagcccag cggccaacag aagcaaaatc
     1201 cagagatcga aattggtgac gagatcccac gccgaccaat gagttttgaa tggccacagc
     1261 acttgcactc caactccgct cgccaggtca agcagtaggt gcccgccagc accaattgca
     1321 caaaccgcta gcgcctcgcg aaaatgcagt ggcgaaaatg atttttccgt caccgcccct
     1381 cgaggatact ttcgatcgag cgcgcaaaat gcacccgcta ggacgcaagc catcaacgcc
     1441 gaaccagcaa gagaatgcaa caccgtgcga tggaatcgaa tgaaggtgtg tcctccgccg
     1501 aaatagctca ggtagtcgag atcgggcgcg atgccggcaa cgatcagcat cgccatgccg
     1561 aaacgcggca ggcggcgctg tcccgcgcgt gcaaaggcca gcgaagttag cgcgtgtgtg
     1621 acaggttcca agatcctgtg tttccagagc tagcgcccgg gtgtcacgat cgatagcgcg
     1681 tctggcacaa tacggaatgc gactggcagt ggcccaacgg gctcgccatc cacctgcgcg
     1741 taaatcggct cgcctcccgc agattcgcaa atcgtttccg ccgatttcca cgcctcaatc
     1801 ccatccatag cacgaagcga gccgaagacc atggctggca ggcacgccac atagcccatt
     1861 cggctccgcc gtgagttcgt cacgacctcg aacgaatcct cgaaaaggct cgcgcccgtt
     1921 gtgattttaa acggcccgcc atagtggacg gtcctgccca ccactatgat ggtggcctta
     1981 cgctcccggc ctgccgagcg gattcgtatt tcaggaaaat cgtattggaa aagttgccga
     2041 aaaccctcaa accaatacgc ggcgattcca gcattcttct tcagcccggg gtgcacgccc
     2101 ttcacaattg cgccatccgg tcccgcgccc gccacggaga gaaaaaatcg tcccttcgac
     2161 gaaagcaaag cggaatgatt tccattcagc ggaaacgccg cgcccagtgc gatccggcgc
     2221 acagttcctc cgtgaatcag ccgggccgcg cgccggatat cccacgggat gcccagttcc
     2281 ttcgccagaa tgttcgctgt gcccgcgggc agcagcgcca tgggaacatc gcttcccgcc
     2341 agcccgttga cgatctcgtt gatcgtgcca tcgccgccgc aggcgatcac cattccgcgg
     2401 cgctgctccg ccgcttttcg cgccacctct gtggccgagc ctgcagctgt cgtggcagca
     2461 agttctgttg cgatgcctgc atccttcaag atccgcgcag cctgctccac gtgggcagac
     2521 cggcgatgcc gtctgcgccc actcgcaggg ttgtaaatca gcagcgcgtc tttgatattg
     2581 tgcggcatgg accggaagat tacgccgata aacgcattgt ataataaacg tcgttcgcgc
     2641 gagatcatca tgtcaaaggc agcaacgata aaaatctcca gtgccgctaa agagatcgaa
     2701 gagctgcgcg aaaaaattcg ccatcacgaa tacctctact acgtcctgga cgatccgaag
     2761 atttcagacg cgaccttcga tcgcctgatg gagcaactca agaagctgga aggtgagaat
     2821 ccgaacctcg taacgcctga ttcacccacg caacgagttg gtggcgcgcc tcgcgagggt
     2881 tttcggaagg tgcggcacaa gactccgatg atgagcctcg ataacgcatt ctcgttcgag
     2941 gccctggccg aattcgatcg ccgcgttcgc cagactaccg gtcgcgaaaa agtcgaatat
     3001 gcctgcgagc agaagtttga cggcttgagc ctttcgctga tttatgagaa gggcaagctc
     3061 gttgcgggcg ttacccgcgg ggacggaaca acgggcgaag acgtgacgcc gaatgtcaga
     3121 acgattcgct cgattccgct gggcatcgac c
//
LOCUS       DATNDT010000255         4091 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1734000, whole genome shotgun sequence.
ACCESSION   DATNDT010000255 DATNDT010000000
VERSION     DATNDT010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1734000"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /gene="hisS"
                     /locus_tag="VN788_08145"
     CDS             complement(<1..865)
                     /gene="hisS"
                     /locus_tag="VN788_08145"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HXU20536.1"
                     /translation="MSEKRQFHAIKGTRDILPPDSALWNSFEQTARNIFESYNFGEIR
                     LPIFEETDLFARSIGAETDVVSKEMFTFESYGAGEPAEEGFRALRSQSLTLRPEATAS
                     VARAYIEHGMHTLPGNAKLYYVGPMFRRERPQKGRYRQFYQIGAEVLGQSDAPAIDAE
                     VLEMLLVLFQRAGLKGTTLYVNSIGCKECRPKYVELLREELRKVKDSLGPDSQRRIET
                     NPLRVLDSKVPAEQDIIEKLPRISDHLCADCREHFAKLKEELNLRDIDFQENWRLVRG
                     LDYYTRTTFEVT"
     gene            1497..3113
                     /locus_tag="VN788_08150"
     CDS             1497..3113
                     /locus_tag="VN788_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXU20537.1"
                     /translation="MSVKSKRKERLSFSEKVSRLATRLRDPEWRRYGKLLILGKATGL
                     AIVLAVIGFMSYFVFAHVYAAAPEVKAADVVNPLNTAWTLIAAFLVFGMQVGFTMLEA
                     GFCRSRETVNVLVECVVDTCLCGILFYAVGFAFMFSHGNGFIGYHWFFLQNAPETYES
                     TGVAFLAVWIFQFAFADTCSTITSGAMIGRTGFVGDLLYSICVTGFIYPVIGHWAWGP
                     DGWLANMGSAGHVWPSLGIGFHDFAGSTVVHTIGGFIALAGAIVLGPRLGRKFKRDGG
                     TPMLPHDLTIAISGGLILWFGWYGFNPGSTLSAMDFAGIGRVATNTTLAACAAGLTSI
                     IYGYFMTKKWDAGFTTNGFLAGLVAITCPCYWVSPTGSILLGGIAGILVILGIDLLEW
                     LRIDDPIGAVPVHGLCGIWGTLSLGLFACGKYGATGPISPDNSAPLRGLFYGGGTQVL
                     VAQIIGSACVAAATFGVAMVVMLLVNATGTLRLPAEAELHGMDLHEHGISAYPEYMIA
                     SFASPSGMPQNSMSSAVEAPNLSKAVARTT"
     gene            3257..3595
                     /locus_tag="VN788_08155"
     CDS             3257..3595
                     /locus_tag="VN788_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXU20538.1"
                     /translation="MTKLEAIVQPGRFEAVKKALIDTGIDGMTVSEVRGHGRQKGHTE
                     TYKGHEYSVDLLPKIKIEIVLDDKLVDSVVDAIIENAATGRIGDGKIFISKIDEAIRI
                     RNQERGVSAL"
     gene            complement(3707..>4091)
                     /locus_tag="VN788_08160"
     CDS             complement(3707..>4091)
                     /locus_tag="VN788_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006748324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20539.1"
                     /translation="VKYGRKGMKLPEEYKVFEQNFVLENWHADVLGQVFGIAGIEYGR
                     ADFGIVGGRVQVYEINTNPHVSADPGSENPTRRMTLAMATKKLCESLAAVDQVRPAGT
                     VRMGGPLLDEWRKKRKWYERPEIRP"
BASE COUNT          846 a         1126 c         1159 g          960 t
ORIGIN      
        1 ctgttacttc aaatgtcgtg cgtgtgtagt agtccaggcc gcggaccagg cgccaatttt
       61 cctgaaagtc gatgtcgcgc aaattcaatt cttctttcag ctttgcgaaa tgctcgcggc
      121 aatcggcgca aaggtgatcg gagatgcgcg gcaacttttc gatgatgtct tgctcggccg
      181 gcactttcga atcgaggacg cgaagcggat ttgtctcaat gcggcgctgg ctatctgggc
      241 caaggctgtc tttcactttg cgcaattctt cgcgaagcag ttccacatat tttggccggc
      301 actccttgca gccgatcgag ttgacataca aagtggttcc cttcaggcca gcgcgctgga
      361 agagcacgag cagcatctcg agaacttcgg cgtcgatggc aggcgcgtca gactggccca
      421 gaacttccgc gccgatttgg tagaactgcc ggtaacgtcc tttctgcggc cgctcgcggc
      481 gaaacatcgg cccgacgtaa tacagttttg cgtttccagg cagcgtgtgc atcccatgct
      541 cgatgtacgc ccgcgcaacg ctcgccgtcg cctccggccg cagcgtgagg gattgcgacc
      601 gaagggccct aaacccttct tccgcgggtt caccggctcc ataactctcg aatgtgaaca
      661 tttcctttga aactacatct gtctcagcgc cgatggagcg cgcgaagagg tccgtttcct
      721 caaagatggg gaggcggatt tcgccgaagt tgtaagactc gaaaatgttg cgcgcagtct
      781 gctcgaatga gttccacagc gccgaatcgg gcggaaggat atcgcgggtg cctttgatgg
      841 cgtggaactg gcgcttttcg ctcattgccg cggcccccgc ggggagaggg aatttggaac
      901 gccgatgtgc gctatcggct cccgcaaaga gtgtcgagta gaagtggcct cgaccttttg
      961 ttttctgtag cttgcaccta tttctactcg gcacgatctg tcgattagaa aggcacactc
     1021 caccgcatgc tggagagcgg cgcgggccgc ggcgctggga gggcaaagag ccgccggcgt
     1081 gaagccccgc gctccgaagc cttccttctc agcgcaagat ttcattctaa aaatcatccg
     1141 aacatagtag agaggcgcgg atgcgcatgc aagcggttgg tggtgtaaag aattgtgtgc
     1201 catttgaaat gtctatcctg cacatcaaaa cgttgcattt tactgatatg acgcttcggt
     1261 atagtctccg gccttcaggt tattcgtagt ttacagagga aagtacgcat ggccgaacgt
     1321 aggccatccc aagggggttc tgtgatcggg cgcagtaaga aattctattc cggaatattc
     1381 agggcgcgac cacggctccc gtgcgattag gggcgagcag tgaatctctt cggaaggcca
     1441 cgacaaaaag caatatcccg gtgcgcacgt tttctttacc tacaaggaga aacagaatgt
     1501 ctgtgaaatc aaagaggaaa gaaaggctat ctttttcgga aaaggtctcg cgactggcta
     1561 cgcggctgcg agatccggaa tggcgccgat acggaaaact cttgattcta ggcaaagcga
     1621 caggtctcgc gatagtgctc gctgtcatcg gcttcatgtc gtacttcgtt ttcgcgcatg
     1681 tctatgcggc agctcccgaa gtaaaggcgg ccgatgttgt taacccgctg aacacggctt
     1741 ggacgctgat cgctgctttc ctcgtcttcg gtatgcaggt gggcttcacg atgctcgaag
     1801 cgggtttttg ccgttcccgc gagacggtga atgtgctcgt ggaatgcgtg gtggatactt
     1861 gtttatgcgg catcttgttc tatgccgtgg gatttgcatt tatgttcagc cacgggaacg
     1921 gttttatcgg gtatcactgg ttcttccttc aaaatgcacc cgaaacttat gaatccacgg
     1981 gcgtggcgtt tctagccgtg tggatttttc agttcgcctt tgctgacacc tgctcgacaa
     2041 tcacctcggg cgcaatgatc ggccggactg gattcgtcgg cgatcttctg tacagcatct
     2101 gcgtgaccgg cttcatttat ccagtaatcg gacactgggc atggggtccc gatggatggc
     2161 tcgccaatat gggcagcgcc ggccacgttt ggccttctct aggtatcgga ttccacgact
     2221 tcgcaggctc cacggtcgta cacacgatcg gcggattcat tgcgctggcc ggcgcgatcg
     2281 ttctgggtcc gcggctcggc cgcaaattca aacgagacgg cggcacgcca atgctgcccc
     2341 acgatctgac aatcgctatc tccggcggac tcattctctg gttcggctgg tatggtttca
     2401 atcccggtag caccctttcc gcgatggact tcgctggcat cggccgcgtg gccacaaata
     2461 caaccctggc ggcatgcgcg gcgggactta cctccattat ttacggttac ttcatgacca
     2521 agaagtggga cgcaggcttt acgactaacg ggtttctggc aggactcgtt gcgatcactt
     2581 gcccgtgcta ttgggtcagc ccgacaggat ctatcctcct tggcgggatc gcgggaatcc
     2641 tcgttatcct gggaatcgac ctgctggaat ggctgcgaat tgacgatccc atcggcgcgg
     2701 tccccgtaca cggcttgtgt ggcatttggg gaacactgtc gcttgggcta tttgcctgcg
     2761 ggaagtacgg cgcgacaggg ccgatctctc cggataatag tgctccgctg aggggacttt
     2821 tctacggcgg gggtacacag gtgctggtag cgcagatcat aggtagtgca tgcgtcgccg
     2881 ccgcaacttt cggcgtggcc atggtggtca tgctcttggt taatgcaacg ggaacccttc
     2941 gcctcccggc agaggcagag ctccacggca tggaccttca cgaacacggg atttcggcct
     3001 accccgagta catgatcgcg tcgtttgctt cgccgtcggg gatgcctcag aactctatgt
     3061 cctctgctgt cgaggcaccg aacttgtcca aggcggttgc acgcaccacc tgaccatagc
     3121 tccggtaaga tgatcccggc ttcaagacgt ctcggccacg tcaggaagcc gggccggaga
     3181 ttgcgcgaat acatgcttgc attgaatcga aatcatcggt cccattaagc agagccgata
     3241 caaacgagga gatgagatga caaagctgga ggcaattgtg cagccgggac ggtttgaagc
     3301 agtgaagaag gccctaattg acaccggcat tgacgggatg actgtatccg aagtccgcgg
     3361 ccacggtcga cagaagggac acacggaaac atataaaggg cacgagtaca gcgtcgacct
     3421 tctaccaaaa atcaaaatcg aaatcgtgtt ggacgacaag ctggtggatt ctgttgtcga
     3481 tgcgattatc gagaacgccg ctactggacg gataggagac ggcaagatct ttatttcaaa
     3541 gattgacgaa gcaatccgca tccgcaatca ggagcgtggc gtctcagcgc tctgaggtta
     3601 atgcacggcc gccccgcagg aagtgggtct aatcacgctg gcggtgattt cggcgggctg
     3661 atgctactgt ttgcaaagcc gttgaaaggc gaatctgcac gggcggctac ggccttatct
     3721 ccggtctttc ataccacttc cttttctttc tccattcgtc gagtagaggc ccgcccatcc
     3781 tcacggtgcc tgcgggccta acttggtcga ccgctgccag gctctcgcaa agtttttttg
     3841 tcgccatcgc aagcgtcatt cgtcgggtcg gattctcgct tcctggatcc gccgaaacat
     3901 gcggattggt gttaatctcg tagacctgca cccgtccgcc gacaatccca aaatccgctc
     3961 ggccgtactc gatccccgca attccaaaca cctgccctag tacatccgcg tgccaatttt
     4021 ccaagacaaa attctgctca aaaaccttgt attcctctgg aagcttcatg cccttccgcc
     4081 catacttcac g
//
LOCUS       DATNDT010000256        17184 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2892336, whole genome shotgun sequence.
ACCESSION   DATNDT010000256 DATNDT010000000
VERSION     DATNDT010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 17184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17184
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2892336"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1263)
                     /locus_tag="VN788_08165"
     CDS             complement(<1..1263)
                     /locus_tag="VN788_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20540.1"
                     /translation="MKSTDEASVEAKIEVLSASLDETRQELAESRQEIKHLRVLLEQL
                     SQELGDQPEVAAVPVVAGKTLAVADVSDEAQTPTPPTPAKITQDDWDILNSRLEQLTQ
                     TRVESGSKFSLKLSGMFLFNTIINSGRVDNFDLPSVAISGATPFGSTGFSLRQSVIGI
                     EGNGPQIVGAKTSADIQADFSGGLGSGYGGSTSGLMRVRLARMRLDWAHTSIIAGLDA
                     PFFSPDSPTSYLTVAEPGFSSSGNLWTWAPQVGVEQRFDARISQFRAEAGILGIPSYI
                     PSLSGNRVPSPGEASRQPVYAVRLSANGRDDSHPYSVGVAGVYLRQRFSGPTTITGGG
                     GMIDWNIPLPSKFELSGELFAGKGLDGFGAAPTPVVAQTDYTEYITYSAAALAHIPSF
                     GGWSQLKFRINSRDEINLAFGGVGRKSGD"
     gene            complement(1748..2824)
                     /locus_tag="VN788_08170"
     CDS             complement(1748..2824)
                     /locus_tag="VN788_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020202954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HXU20541.1"
                     /translation="MRFSSTKFKPAHANLRGRIFGIYGILIAFNVSAWFWAVLTFRAS
                     PVLVGTALLAYTLGLRHAVDADHIAAIDNVTRKLVQAGKRPVGVGFMFSLGHSTIVLV
                     GAAAIAAAALTLQDRMDAIRDIGGLIGTLVSAFFLFAIGAVNLVVLRSIYRAFTAVRS
                     GKPYVEEDFDMLLGNRGFLSRLFRPMFQIITESWHMYPLGALFGLGFDTATEIGLLGI
                     SAAGASQGMSFISILIFPILFAAGMSLVDTTDSVLMLGAYGWAFVKPVRKLYYNMTIT
                     AISVVAAFAVGGIEVLGLLVGRFHLRNGLLIATAQLNDHFGSLGYFIVSLFALCWIIS
                     IAIYAWSGFDHTAPIKTRRQVSSD"
     gene            complement(2923..3630)
                     /gene="hypB"
                     /locus_tag="VN788_08175"
     CDS             complement(2923..3630)
                     /gene="hypB"
                     /locus_tag="VN788_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HXU20542.1"
                     /translation="MNSQPRIVEVRKNILKQNDVIARRLRERFQHEGIFVMSLVSSPG
                     SGKTLFLEKLMSVLRHKYRVAALVGDLATENDALRLARSGAPVKQITTGTLCHLEASM
                     VEQALNGWDVGPLDVLFVENVGNLVCPSSYDLGEKLRVVLMSVTEGEDKPLKYPTIFN
                     GADLAVITKVDLAAAAEFDSLSLERNLRLVRPDIRIFYVSAKNGDAMDSCVDFLEAQI
                     RTLRAPLHAERQIQAVT"
     gene            complement(3627..3974)
                     /gene="hypA"
                     /locus_tag="VN788_08180"
     CDS             complement(3627..3974)
                     /gene="hypA"
                     /locus_tag="VN788_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HXU20543.1"
                     /translation="MHELSIALSIIEIVEEEMQSRGAQQILAVHVRLGALSGVVKDAL
                     IFSYDTASEQTALAGSRLVVEEVPVVMDCPECGQRREVRSPQQLCCATCGTSALDIVQ
                     GRELLVSGLEVLE"
     gene            complement(3978..6308)
                     /gene="hypF"
                     /locus_tag="VN788_08185"
     CDS             complement(3978..6308)
                     /gene="hypF"
                     /locus_tag="VN788_08185"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013876969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HXU20544.1"
                     /translation="MSSQPRSKGWIKVITARSIRVKGIVQGVGFRPFVFREALANGLN
                     GWVANTADGVDIFLEGPEEGLDLFVRQLKNQHPPAACIASVDVQCAEWEGFEKFSILQ
                     SQRTNHVTARISPDLAVCDDCLRELADPKERRYRYPYINCTNCGPRYSILRKLPYDRS
                     NTTMHAWPMDEYCTREYNDPSNRRFHAQPVACSGCGPSYFLEFENETIRENNTSVRRA
                     AELLREGKIIAVKGIGGYHLACDANNLATVQLLRERKYRKERPFAVMARDLKQAQAIV
                     ELNHESEALLCSAVRPIVLANAKVSNAEVAPDNADIGVMLPYTPLHHLLFAENAPEVL
                     VMTSANRSSEPIAFDDDDARRRLSGIADAFLIGERPIARRVDDSIVRVGAFGRMTLRR
                     ARGLAPGSVATIPTDRPILALGADLKNAITLVVDGQAFVSQHIGDLHHHDCLLSFEQT
                     IDDLLAMYALRLEDVTVVHDLHPQYTSTAFAQARPSDAIGVQHHRAHVASVLAERCCL
                     HERVLGVAFDGTGYGDDGTIWGGEIFCGSVSGGFQRIAHLRNAWLAGGDAAAEHPVQA
                     ATGFLSQMQKLPDLRDAPFNFPRVYEYSLQMVNKRVRTFTTSSMGRLFDAAAALLGFT
                     RNSTFEGQAATWLEHQARKSSRVEAYPFPLQGHELDFRPLLQSIVEERVRGRCPAEIA
                     RAFHLGIASGLCEAIKCFCVRERVQIVVLSGGVFQNDMLLTDIKSLLDSEPLCVWTNH
                     DVPANDGGISLGQAALAAFHVDHSASIVQAQTAQRS"
     gene            complement(6329..6553)
                     /locus_tag="VN788_08190"
     CDS             complement(6329..6553)
                     /locus_tag="VN788_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxx-star family glutaredoxin-like
                     (seleno)protein"
                     /protein_id="HXU20545.1"
                     /translation="MGYELYGSPGCPYTRDVREWLEWEGAEFVEYDVETDVGARKRML
                     QISNGQRTVPVLTLDGAVVQIGWQGRGCVV"
     gene            complement(6543..7724)
                     /locus_tag="VN788_08195"
     CDS             complement(6543..7724)
                     /locus_tag="VN788_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013987172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HXU20546.1"
                     /translation="MAITHSIGKSAAQHLLARNEIIENFFASEALPLANACREMSERF
                     LRGGRLLAFGRGAYASDAQHVSVEFVHPIIVGKRALPALDLSIHFRPWIESLVHADDM
                     VMGFGPPEGDLEVQAALDFATKRRAMTFALPGNPATYSLTAVTSNAFIHQELVEILYH
                     TLWETVHVFFEHRELGQDVGAAEFLYPFLGQQKQETTDAISDVAASIRMKAEDDSKLR
                     AQVARDESEEINRAVASIGERLQRGGKLIFFGNGGSATDANDAALDCVAPPAGWAAVP
                     AISLAMEPANVSAIANDVGVQLIFLRQLVAHARPEDIAIGLSTSGGSENVIVALKEAR
                     RRGLLTVALLGYDGGEIKRNSLADFPIIVRSDYIPRIQEVQGSVLHVIREMLEENRHG
                     L"
     gene            complement(7743..8783)
                     /gene="hypE"
                     /locus_tag="VN788_08200"
     CDS             complement(7743..8783)
                     /gene="hypE"
                     /locus_tag="VN788_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013400898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HXU20547.1"
                     /translation="MAVAVSKVRFSDPQIEMAHGAGGKASRRLVEGLIAPLLLSSPGD
                     LLGDAAHVQIDGVRVAITTDSFVVKPLRFPGGSIGELAVNGTVNDLAVSGAKPEAIVA
                     TFVIEAGLPIEVLDAEVRAMAEAARRAGVRIVGGDTKVVEHGKADQIYISTTGIGRSM
                     PNVHVDPRAVKSGDKILISGPIGDHGITILLARGELDIEADLRSDTRSVLPMVEALAH
                     AAGSGIHWMRDPTRGGVATSLNELAHDCRLGITIDESSVPVGDAVRGACELLGLDPLH
                     IANEGQFLAVIAAEQADAALKALQSVPGAEDAAIIGEIREQPAGALLASTKYGATRIV
                     DMLVGDPLPRIC"
     gene            complement(8804..9916)
                     /gene="hypD"
                     /locus_tag="VN788_08205"
     CDS             complement(8804..9916)
                     /gene="hypD"
                     /locus_tag="VN788_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HXU20548.1"
                     /translation="MKYVDEFRDPELIRKTTQEIRRLVDPQRQYRLMEVCGGHTHAIY
                     RFGLKDILPENIDLIHGPGCPVCVLPMGRIDDGLSIAKSNDVIFAAFGDMMRVPGAHG
                     SPLECKARGMDVRIVYSPADALKMAKANPTRHVVFFAIGFETTAPSTALVIMRAKQEG
                     VRNFSVFCNHVTIIPAIRAILDSPDMRIDGFIGPGHVSTVIGCRPYEWIARNERKPIV
                     VSGFEPLDLLQSIVMLLRQLQKNEYRVENQYQRVVPWEGNRGALKAMAEVFQVRPYFE
                     WRGMGFISQSALRIRDAYAEWDAEVRFSVEGLRVTDPKAAQCGEVLKGVLKPHQCKLF
                     GTECTPEHPIGALMVSSEGSCAAYHNYEHRKLVALS"
     gene            complement(9952..10257)
                     /locus_tag="VN788_08210"
     CDS             complement(9952..10257)
                     /locus_tag="VN788_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005588665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HypC/HybG/HupF family hydrogenase formation
                     chaperone"
                     /protein_id="HXU20549.1"
                     /translation="MCLAIPGKILEASTTENDATALVEVAGVRRRVDLGLLEDRPRPG
                     DWVLIHVGFAMSKISEQDAIDQMRTLAMLGEAEAAMTEVRGYGSGDETSAEPPQGSK"
     gene            complement(10335..10460)
                     /locus_tag="VN788_08215"
     CDS             complement(10335..10460)
                     /locus_tag="VN788_08215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20550.1"
                     /translation="MKSNARSVGWKLVKAAVVVGAGAMFVSMWPDLKRYVKIERM"
     gene            complement(10457..10972)
                     /locus_tag="VN788_08220"
     CDS             complement(10457..10972)
                     /locus_tag="VN788_08220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HXU20551.1"
                     /translation="MKILVGGIGNIFFGDDAFGIEVVRRLSDCGVSANVCVKDFGIRG
                     FDLASALCEEWDLVILVDSMSRGGEPGVLYVIEPDLSEGSKDAGATMDPHGLNPMYAI
                     DLAKTMGTISARVMLVGCEPDTFESEGEVFALSAAVAAGVETAVEAIRRILDEIQESQ
                     QCVASSSGGSR"
     gene            complement(10969..12183)
                     /locus_tag="VN788_08225"
     CDS             complement(10969..12183)
                     /locus_tag="VN788_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20552.1"
                     /translation="MMQLDELRKLSSTLLYEGCLLYPYRASALKNQRPGWTFGALLPR
                     AYVAENPGESDLMQAQVLARTSGDAMLELEVRFLQLDADGPESGGVIERCVNVQAELK
                     SLLLQNSQTSFEFPVPGFEGDRIQGSAEVGAEHTTGNIFRYTVRVRNMSGMPDGACAS
                     RDAALNYSLLSVNGLLTIHMGEFTSSLEPPDELREEAARCKQVGVYPVLAGDVQTRAT
                     MLISPIILYDFPQVAPESKGDFFDSSEIDEMLALRVLTLSDAEREEIVQRAPDSARQI
                     LNRTTNLSESEIRGMHGAFRAPKNNAVNEWSDEDMARLETICVSGVDLRKGDRVRVWP
                     QNRADIFDIALQGKEATIEALEQTFEGEIYVAVTIDDDPGRDLGEMRQIGHRFFFRAE
                     EIELLSPKVAGT"
     gene            complement(12180..12836)
                     /locus_tag="VN788_08230"
     CDS             complement(12180..12836)
                     /locus_tag="VN788_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6084 family protein"
                     /protein_id="HXU20553.1"
                     /translation="MPDLLFKIEDVKAMSDSATPVLVFSLRVENTLKDEDVRSVSLNC
                     QLHIHPARRRYEAAEQSALADLFGERERWGKTLRRLYWTGVTVTVPAFCANTVVDVRV
                     PCTFDFNIAATKYCAALEQGDIPVVWVFSGMVFYLANGALQMAPVSWSREAEFRIAAA
                     VWKEMMALYYPNSAWLSLRKDVFERLRRYKARHGLLTWEQAMEHVLAAAGDTAETRVQ
                     "
     gene            complement(12817..13473)
                     /locus_tag="VN788_08235"
     CDS             complement(12817..13473)
                     /locus_tag="VN788_08235"
                     /inference="COORDINATES: protein motif:HMM:NF040271.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5947 family protein"
                     /protein_id="HXU20554.1"
                     /translation="MGAPDQPSLDAGGFAALKRLRRMPQAAEICEICSRDLGAEHPHA
                     IDPRERRIVCTCDACALLFESSTSMRYRRIPRDAFHLRNFEIDDVEWESLSIPIGLAF
                     FFESSAMKRVTAVYPSPGGAVQAEVPQAVWQEITQRHPRVQQMQSDVQALLVNRLGDS
                     RRYFLAPIDRCYELTGLLRKYWSGFTGGDELWAEVHKFFKRLAEQAIGVGAMDARPAV
                     "
     gene            complement(13494..13691)
                     /locus_tag="VN788_08240"
     CDS             complement(13494..13691)
                     /locus_tag="VN788_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20555.1"
                     /translation="MPETYTSKTQLSSHTEGTKRGEERLQSGGLEAGREGDVPTARSS
                     TSINPKTHDPIDPRMPHIHPA"
     gene            complement(13704..14270)
                     /locus_tag="VN788_08245"
     CDS             complement(13704..14270)
                     /locus_tag="VN788_08245"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HXU20556.1"
                     /translation="MSSNREDQASESLHIERQVERLGSIADPAARAAATDLVASVLEL
                     HRCALERMLAIVKQNGSAAAVFAAFEQDELVRSVLLLHDLDRRGIGERVVEMLAELRP
                     KMEKFGAVAEVIQADENAVRVRVSGMEGGCGSTAETVRSFVEASLIGIAPDAAEITVE
                     LAVVDKTFVPINSIRPAAVSFEPAAAKS"
     gene            complement(14267..16072)
                     /locus_tag="VN788_08250"
     CDS             complement(14267..16072)
                     /locus_tag="VN788_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HXU20557.1"
                     /translation="MAVTTDTSLAGQKGNRTLVDMNWDPITRIVGSLGIYTKIDFNNR
                     EVAECYSTSSMFRGYSVFMRGKDPRDAPFITSRICGICGDNHATCAVYAQNMAFGVKT
                     PPTGEWIINLGEAAEYMFDHNIFQDNLVGVDYCEQMVKATNPGVLEKANNTEAPHSKD
                     HGYRTIGDIMRALNPFTGEYYREALHMSRLTREMFCLMEGRHVHPSTLYPGGTGTVAT
                     IQLFTDFLVRLMKYCEYMKKALPLHDDLFNFFYEALPGYEKVGQRRVLLGCWGSWQNP
                     DTCDYKYENMGSWGKDMYVTPGVIVDGELVTNDLIDINLNMRILLGSSYYEDWEDKET
                     FVRQDPIGNPIDKRHPWNQSTTPWPQKRDFNKRYSWVMSPRWLLKRTGEHLALDTGGG
                     PIARLWSTALSNKVDIGYVKATGRSVKITLPKTSALPEKEYEWTIPKWSNAIERNRAR
                     TYFQAYAAAAAMYFVERAIEEVQKGHTKTWAPFEVPEEAIGVGFHEAVRGVLSHHVVI
                     RNKRIANYHPYPPTPWNANPRDIYGTPGPYEDAVQDMPIFEENGPADFKGVDIMRAVR
                     SFDPCLPCGVHMYTGKGKLLEARHVPMFGLMDHGG"
     gene            complement(16056..17156)
                     /locus_tag="VN788_08255"
     CDS             complement(16056..17156)
                     /locus_tag="VN788_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression protein HypE"
                     /protein_id="HXU20558.1"
                     /translation="MASPSLSSLKPKGPAPAEVHVIWLPDGMSCDGDTISVTAAEQPS
                     IEDLILGAIPGVPKVVLHNRVLAAEQGGEEFMSWLYKAERGELSPFLLVVEGSIPNEK
                     IKREGYWTGIGNDPDGQPITLCDWIDRLAPKALAVVAAGTCATYGGIHAMAGNPTGSM
                     GLADYLGWDWRSSAGLPIVNVPGCPVQPDNMTETLLYLCFQAANLAPIIPLDEMLRPT
                     WLFGKTVHEGCDRGGYYEQGDFAHEYGSPKCLVKIGCWGPVVNCNVTKRGWMRGIGGC
                     PNVGGICIGCTMPGFPDKFMPFMDEPPGARLSAGTVGIYGTMIRSLRSITNNSSNREP
                     KWRHKGTTLSTGYVPTTYGNGMRKDGANGSHH"
BASE COUNT         3864 a         5203 c         4360 g         3757 t
ORIGIN      
        1 atcgccggat ttccgtccca caccgccaaa cgcgaggttg atctcgtcgc gcgagttgat
       61 tcggaatttg agttgagacc agccgccgaa actcggaatg tgcgccagcg cggcggcgct
      121 atacgtaatg tactcagtat aatccgtttg agccactacc ggggtgggcg cggctccaaa
      181 gccgtcgagc cccttccctg cgaataattc accgcttaac tcaaattttg agggcagcgg
      241 aatattccaa tcgatcatgc cgcccccacc cgtgatcgtc gtcggccccg aaaatcgctg
      301 ccgtaaataa actcccgcca ctcccactga ataaggatgc gaatcgtccc ttccgtttgc
      361 cgatagcctt accgcgtaaa ctggctgtcg agatgcctct cctgggctgg gcactcggtt
      421 tccggaaagc gacggtatgt aagaaggaat ccccagtatt cccgcctcgg cgcgaaattg
      481 cgaaatcctc gcatcgaagc gctgttccac cccaacttgc ggggcccaag tccaaagatt
      541 gcccgacgaa gaaaagcccg gctctgccac agtcagatat gatgtcggcg aatcgggcga
      601 gaaaaaggga gcatccaatc ccgcgataat tgacgtgtga gcccaatcca gcctcatccg
      661 tgccaaacgc acgcgcataa ggccggaggt cgaaccgccg taacctgagc caaggccgcc
      721 cgaaaagtca gcttgaatat cggcagacgt tttcgcgccc acgatctgcg gcccattacc
      781 ctctattccg atcaccgact ggcgcagcga aaacccggtg cttccaaacg gagtcgcacc
      841 tgatatcgca acacttggta ggtcgaaatt gtccactcgg cccgagttga tgatcgtatt
      901 gaacaaaaac attccggaaa gtttcagcga aaacttcgat ccgctctcta ccctggtttg
      961 cgtgagttgt tccaagcggc tatttaagat gtcccaatcg tcctgcgtaa ttttggcagg
     1021 agttggcggg gtcggtgtct gcgcctcgtc ggatacgtcc gccacggcga gtgttttccc
     1081 ggccaccacg ggtaccgcag ccacttctgg ctgatcaccg agctcttggg aaagttgctc
     1141 aagtaagact cgaagatgtt tgatctcctg gcgggactca gccagttcct gtctggtctc
     1201 gtcgagcgat gcgctcagaa cctcaatttt tgcttcaacg gacgcttcat cagtgctttt
     1261 cacgggggct gaaggcgact gggccatcgc ggtgctacct acaaggaaga ggaacagtgc
     1321 gccctcgcag cgaaacatac gacatatata tccattcgcg agagcgcgca taaggcctcc
     1381 aaacagcgat cagaggaaga ttggggcgcc aaagccaaaa caccccacga tcatccctga
     1441 agtgatgcct actttagttc aaagatgtca gaattgtgcg ttacgtgtga tattttgacg
     1501 ttttgttgac attcttttga caagaaatca caggtacaga agtttatagg tcttggagtg
     1561 tccgttggtg agtggggtca gagaattggg agtgcgctta ctggaaccta ttcgaacgca
     1621 ccttttacgc ctctcaatga ggtgaatatt gcagtttttc atgcggaaac gccaaagaaa
     1681 tcgtcgccaa aggcatcgcc agagtatcaa agaccggtaa tcttaatgtt agaagcaatt
     1741 tcactgctca atctgaggat acttgccgtc tggtcttgat cggtgccgta tgatcgaagc
     1801 cgctccacgc gtagatcgcg atcgaaatga tccagcagag ggcgaagagg ctaactatga
     1861 agtagccaag cgagccgaaa tggtcattca gttgcgcggt agcgatgagc aacccgttcc
     1921 ttaagtgaaa tcgaccgacg agtaggccca atacctcaat gcctccgacg gcaaatgccg
     1981 cgacaacgga aatggccgta atcgtcatgt tgtaataaag cttgcgaacg ggctttacga
     2041 atgcccatcc atatgcgccc agcatcagca cgctgtctgt tgtatcgacg agcgacatgc
     2101 ctgcggcaaa cagtatggga aaaatgagaa tggatatgaa agacattccc tgggacgcgc
     2161 ccgcagccga tatccccaaa agaccgatct ctgtagcggt gtcaaaaccg agcccgaaca
     2221 atgcgcccag gggatacatg tgccagcttt ccgtaatgat ttggaacatc gggcgaaaca
     2281 gccgcgaaag aaaccctcga ttgcccagca acatgtcaaa gtcttcttcg acgtacggct
     2341 tccccgaacg caccgccgtg aacgcgcggt agattgatcg tagaacgact agattgacgg
     2401 caccgatcgc gaagaggaaa aatgcggaaa ccaatgtccc gattaggccg ccgatatccc
     2461 tgatcgcgtc cattcggtct tgtagcgtca gtgctgccgc agcgatcgcc gccgcgccta
     2521 ccaggacaat cgtcgaatgg ccaagcgaga acataaatcc tacgccgaca ggacgcttgc
     2581 ctgcttgcac caatttcctt gttacgttgt cgatcgctgc gatgtgatcc gcatccaccg
     2641 catggcgcaa accaagggta taggcgagca gagccgttcc gaccaaaact gggcttgcac
     2701 gaaaagtgag cacagcccaa aaccacgcgg acacgttgaa cgcgatgaga attccatata
     2761 tcccgaaaat tctgccgcgt aaattggcat gtgcaggctt aaatttagtt gagctaaatc
     2821 tcatcgctct ctttcaaacc cgtttactgt atcgatttca aacacaagga gaaacaggac
     2881 cacgactcaa accaagtgtc caacggtcat ctcagtctca gactaggtaa cagcctgtat
     2941 ctggcgctcg gcgtgaagcg gcgctcgcaa ggtgcgtatt tgcgcctcca gaaaatcgac
     3001 acacgaatcc attgcgtcac cgttcttggc gcttacataa aaaatgcgta tatccggtcg
     3061 tactaatcgc agattgcgct caagagataa cgaatcaaat tcggccgcag ccgccaagtc
     3121 caccttcgta ataacagcca gatcggcgcc attgaaaatg gttggatatt ttaacggctt
     3181 gtcctctccc tcggtgacgg acatgagcac gacacgcagt ttctctccga ggtcgtaaga
     3241 cgaagggcaa acaagattgc ctacgttttc gacgaagaga acatccagcg gcccaacgtc
     3301 ccatccattg agcgcctgct cgaccatcga ggcttccaaa tggcacagcg tgccagtggt
     3361 gatctgcttt accggagcgc cactgcgagc gaggcgcaac gcatcattct ccgttgcaag
     3421 atcacccacc agggctgcca cgcgatactt gtgccgcaac accgacatta gcttttcgag
     3481 gaacaaagtt ttgccggagc cggggctgga gaccaggctc atcacgaata tgccttcgtg
     3541 ctgaaaccgc tcgcggagcc ttcgtgcaat cacgtcattc tgcttcaaga tgttctttct
     3601 aacttccacg atacgaggct gcgaattcac tccagcacct ccagtccact gaccagtaat
     3661 tctcttccct gcacaatatc caacgccgag gttccacacg ttgcgcagca gagttgttgt
     3721 ggcgaacgaa cttcgcgccg ttgtccacat tccgggcagt ccatcaccac cgggacctcc
     3781 tccactacca accgcgatcc agccaaagca gtttgttcac tcgcggtgtc gtacgagaag
     3841 atcagcgcat cctttacgac gcccgagagc gctccaagcc taacgtggac ggccagtatc
     3901 tgctgggcgc cgcgagattg catttcttct tcgacaattt caataatgct cagcgcgatc
     3961 gaaagctcgt gcattcgtca ggacctctgt gcggtctgtg cttgaacgat actcgcactg
     4021 tgatccacat ggaacgcagc caacgccgct tgcccaaggc tgatcccgcc atcgttggcg
     4081 ggtacgtcat gattggtcca cacacacaac ggttcagaat ccaataagga tttaatatct
     4141 gtcagcagca tgtcattctg gaagacaccg cctgagagaa ccacgatctg gacacgttcg
     4201 cggacacaaa aacacttgat cgcctcgcat agcccgctgg caattcccaa gtgaaacgcg
     4261 cgagcaatct cggccggaca cctccctcgc acgcgttcct ccacaatgga ttgaagcagc
     4321 ggccgaaaat cgagttcatg tccttgcaga gggaatggat acgcttcgac tctggatgac
     4381 tttcgcgctt gatgctcaag ccaagtcgcc gcttgacctt cgaatgtact gttgcgggta
     4441 aagccgagaa gtgcagcagc cgcatcaaag aggcgaccca tagaggaagt cgtgaacgtg
     4501 cgtacacgct tgttcaccat ttggagtgaa tactcataaa cccgcggaaa attgaatggt
     4561 gcatcgcgca agtctggcag tttttgcatt tggctgagaa aaccagtcgc ggcctgaaca
     4621 gggtgttctg ccgccgcatc gcctccggct aaccatgcgt ttcgcaggtg ggcaattcgc
     4681 tgaaatccgc ctgaaacgct gccacaaaat atctcgccgc cccagattgt cccgtcgtcg
     4741 ccgtagcccg ttccatcaaa agcgacacca agcacccgct catgaaggca gcagcgttca
     4801 gcaaggacgg aagcgacgtg cgcgcgatga tgctgcacac ctatcgcgtc tgagggtcgc
     4861 gcctgagcga aagccgttga cgtgtactgc ggatgcaaat cgtgtacgac ggtcacgtcc
     4921 tccagtcgga gcgcatacat cgcgagtaag tcgtcaatcg tctgctcgaa agagagcaag
     4981 cagtcatgat ggtgcaggtc tccaatgtgt tgactgacaa atgcctgacc atcgactacc
     5041 agggtgatcg cgtttttgag gtccgcgccg agcgcgagga taggtcgatc ggttggaatt
     5101 gtcgcaaccg aacctggtgc taatccacgg gcgcgccgaa gcgtcatgcg cccgaaagcg
     5161 cccactcgta cgatggaatc gtcaacgcga cgcgcgatcg ggcgttcccc aatcaggaac
     5221 gcatcggcaa tgcccgagag tctccgcctc gcgtcgtcat catcaaaagc aatcggctcg
     5281 cttgatcggt tcgcgctcgt catgacgaga acttctggag cattctccgc aaacaaaaga
     5341 tggtgcagcg gcgtgtacgg cagcatcact ccgatatcgg cgttgtccgg cgcaacctcg
     5401 gcgttagaga ctttggcgtt agcaagcacg atcggcctaa ctgccgaaca aagcaacgcc
     5461 tcggactcgt ggttcaattc gacgatggcc tgagcctgct tcaagtctcg cgccattacg
     5521 gcaaacggcc tttctttacg atatttgcgt tccctcagca attgcaccgt cgccaggttg
     5581 ttcgcatcac aagccaagtg atagccgcca atgcccttca cggcaataat cttgccctcg
     5641 cgcaaaagtt ctgcggcgcg cctcacgctt gtattgttct ctcggatcgt ctcgttctcg
     5701 aattctagaa aataagaagg tccgcaacct gagcacgcca caggctgcgc gtgaaagcgg
     5761 cgattcgatg gatcgttata ttcccgggtg cagtattcgt ccatcggcca tgcgtgcatt
     5821 gtcgtattcg agcggtcgta cggaagtttg cgaagaatgc tgtagcgtgg cccgcagttc
     5881 gtgcagttta tgtatggata cctgtagcgc cgctctttcg gatcggccaa ctcgcgcaga
     5941 cagtcatcgc aaaccgcgag atcgggcgat atacgcgcgg tcacatgatt tgttcgctgg
     6001 ctctgcagaa tggagaattt ctcgaaacct tcccactccg cgcactgtac atcaacggac
     6061 gcgatgcacg ccgctggcgg gtgttgattt tttaattgcc ggacaaaaag gtccaggcct
     6121 tcttcgggtc cttcgaggaa aatgtccact ccatcggcgg tgttcgcaac ccatccgttt
     6181 aatccattag ccagcgcttc gcgaaataca aaaggacgaa aaccaacgcc ctggacgata
     6241 cccttcacac gaatcgagcg cgctgtaatc actttgatcc aacccttgct tcgtggctgc
     6301 gaggacatcg agaattgaag tgcaccgcct aaacgacgca ccctcgcccc tgccacccga
     6361 tttgcacaac cgcgccatcg agcgtcagca caggaacagt gcgctgtccg ttcgaaattt
     6421 gtagcatgcg tttccgagct cccacgtcgg tctcaacgtc atattcaaca aactcggcgc
     6481 cctcccactc aagccactcg cgcacatctc gcgtgtacgg acatccgggg ctgccgtaaa
     6541 gctcatagcc catggcgatt ttcctcgagc atttcgcgaa tcacgtggag caccgaaccc
     6601 tgaacctcct ggattctcgg aatgtagtcg ctgcgaacga taatcggaaa atccgcgagc
     6661 gagttgcgtt tgatttcgcc gccgtcataa ccgagtaagg ccacagtcaa gaggccccgc
     6721 cgccgtgctt ccttcagcgc aacaatcacg ttttcagaac cgccgctggt cgaaagccca
     6781 atcgctatat cttcggggcg cgcgtgcgcc accaactggc gcaagaaaat caactgaact
     6841 ccaacgtcgt tcgcaatcgc gctcacgttt gccggctcca tcgcgagcga gatagcgggc
     6901 actgcagccc agcccgcagg aggcgcaacg caatcgagag ccgcgtcatt cgcgtcggta
     6961 gcggaaccgc cattaccaaa gaaaatcaac ttgccgccac gctgcaaccg ctcaccgatg
     7021 gacgccaccg cacggttgat ctcttccgat tcatcgcgcg cgacttgggc gcgaagcttt
     7081 gagtcatctt ccgctttcat tcggatcgac gcggctacgt cgctgatagc atcggtagtc
     7141 tcctgttttt gctggccaag aaacggatag agaaattccg cggcgcctac gtcctgcccg
     7201 agttcgcggt gttcaaaaaa gacgtgcacc gtctcccaaa gcgtgtggta gaggatctcg
     7261 actagctcct ggtggatgaa ggcgttggag gtaaccgcag tcagcgagta tgttgccgga
     7321 tttccaggca gcgcaaaagt cattgcacgg cgcttcgttg caaaatcgag tgccgcttgc
     7381 acttccagat cgccttccgg cgggccaaaa cccatcacca tatcgtccgc atgcacgagc
     7441 gactcgatcc aggggcgaaa gtgtatggat aaatcgagcg ccggcaacgc gcgctttccc
     7501 acaatgatcg ggtgcacgaa ttcgactgag acatgctgtg catcgctggc atacgctcct
     7561 cgcccgaatg ccagcagccg ccctcctcta agaaagcgct cggacatctc ccggcaggca
     7621 ttggcgagcg gcaacgcttc gctcgcaaaa aagttctcga tgatttcgtt gcgcgccaac
     7681 agatgttgcg ccgctgactt tccgatactg tgcgtgatag ccattacgtt tctgtttgac
     7741 tctcagcaaa tcctgggaag aggatcgccc acgagcatgt cgacgatccg cgtagcacca
     7801 tatttcgtgc ttgcgagcaa tgcgcccgca ggctgctcgc ggatttcgcc gatgatcgcc
     7861 gcgtcctctg cacctggaac agactgcagc gccttaagcg cggcatccgc ctgctccgcg
     7921 gcaataacgg cgagaaactg cccttcgttt gcaatatgca gtgggtccag gccaagcaat
     7981 tcacaggcac cgcggactgc atctcccacg ggaacagagc tctcatcgat cgtgattcca
     8041 agccggcagt cgtgagcgag ttcgttgagc gaggtggcta ctccgccgcg cgtcggatcc
     8101 cgcatccagt gaatgccaga tccggccgca tgcgcgagtg cttcgaccat cggcagcacc
     8161 gaacgcgtat cggatcgcaa atccgcttcg atatcgagtt cacctcgtgc cagtagaatc
     8221 gtgatgccgt gatcgccgat cggcccggaa atgaggattt tgtcgcctga tttcaccgca
     8281 cgagggtcga cgtgaacgtt gggcatgctg cgaccgatac cggtcgtgct gatgtaaatc
     8341 tggtcggctt tgccgtgctc cacaactttc gtatcgccgc caacaattcg tactcccgcg
     8401 cgccttgccg cttcagccat agcgcgcact tccgcatcaa gaacctcgat aggcaatccg
     8461 gcctcgatca cgaatgtcgc cacgatcgct tcgggtttcg caccggaaac agccagatcg
     8521 ttcacagttc cgttgacggc cagttccccg atggaacctc caggaaaccg caacggcttt
     8581 acaacgaaac tgtccgtagt gatggcgaca cgaactccgt caatctgcac atgggccgca
     8641 tcgccaagca ggtcgcctgg agaagagagc agcaacgggg cgatcaaacc ttccacgagc
     8701 cgccgactgg ccttcccgcc tgcgccatga gccatttcga tctgcggatc cgaaaaacgg
     8761 actttcgaca cagctacggc cattactgac acctcgagct gagctagctt aacgccacga
     8821 gcttgcgatg ttcataattg tggtaagcgg cgcaagagcc ctccgatgac accatcagcg
     8881 cgccgatcgg atgttccggc gtacactcgg tcccaaacag cttgcattga tgtggcttca
     8941 gtacaccctt cagcacttcg ccgcactgcg cagcctttgg atcggtgacg cgaaggcctt
     9001 ccaccgaaaa acgaacttcg gcgtcccatt cggcgtaggc atcgcggata cgcaaagcgg
     9061 attgcgagat gaatcccatt ccgcgccatt caaaatacgg tcttacttga aacacctcgg
     9121 ccatcgcctt gagcgcgcct cgatttcctt cccacgggac cactcgttgg tattgatttt
     9181 caacgcgata ctcgtttttt tgcagttgcc gcaacagcat cacgatcgac tgcaacaggt
     9241 cgaggggctc gaatccggaa actacgatcg gtttgcgttc attgcgtgcg atccattcat
     9301 aagggcggca gccaatgacc gtggaaacgt gtcctgggcc gatgaatcca tcgatccgca
     9361 tatcgggcga atcgagaatc gcgcgaattg cgggaatgat cgtcacgtga ttgcagaaca
     9421 ccgaaaagtt gcgaacgcct tcctgctttg cgcgcatgat cacgagcgcg gtcgatggcg
     9481 ctgtagtctc aaagccaatc gcgaagaaca ccacatgccg cgtcggattg gccttcgcca
     9541 ttttcaacgc atcggcgggc gaataaacga tgcgcacatc catcccgcgt gccttgcatt
     9601 ccagcggact gccgtgtgcg ccgggaacgc gcatcatgtc gccaaatgcc gcaaaaatta
     9661 catcattcga tttggcgata ctcagcccat cgtcgatacg gcccatcggt agcacgcaaa
     9721 ccgggcaacc cggtccgtgg ataagatcga tgttctccgg aagaatgtct ttcagcccga
     9781 agcggtaaat cgcgtgggta tgtccgccac acacctccat taggcgatat tggcgttgtg
     9841 gatcaacgag acggcgaatc tcctgcgtcg tcttacggat taattctgga tcgcgaaact
     9901 catcgacata tttcattttt cacttcacgc ggcctaatac ggcagaagcg ttcacttgga
     9961 accttgcggg ggttcggcag atgtctcatc gccggaccca tatccgcgaa cctcagtcat
    10021 cgctgcttcg gcctcgccca acatcgcgag cgtgcgcatc tgatcgatgg catcctgttc
    10081 gctgattttg ctcatggcaa agccgacgtg aatcagcacc cagtcgccgg ggcgcggccg
    10141 atcttctagc aaccccaaat caaccctgcg gcgcacgcca gctacttcga ccagtgccgt
    10201 cgcatcgttt tccgttgtgg aagcttcaag aatttttccg ggaatggcca ggcacatcgg
    10261 ctctccttaa aaatcgcctt atcaaaggtc cccgcacgga gccgccgcca gacgcgcgcc
    10321 gcgccaagga tggcctacat gcgctcaatc ttgacgtacc gttttaagtc cggccacatg
    10381 gagacaaaca tggcaccggc gccgaccacg actgccgctt tcacaagctt ccatcccaca
    10441 gaacgggcgt tggatttcat ctgcttcctc ctgaacttga cgcaacgcat tgttgcgatt
    10501 cttgaatttc atcaagaatt cggcggattg cctcgacggc tgtttcaacg ccagccgcca
    10561 cggctgcgct cagcgcgaac acttctcctt cactttcaaa agtgtcaggt tcgcacccca
    10621 ccagcattac gcgcgcggat atggtgccca tcgttttcgc caaatcgatt gcatacatag
    10681 gattaagccc gtgtgggtcc atagtggccc ccgcatcctt cgatccctcg ctcaaatcgg
    10741 gttcaatcac gtacaaaacc ccgggttctc ctccgcggct cattgaatcg accaaaatca
    10801 cgagatccca ttcttcacaa agggcggaag ctaaatcgaa tccgcgaatc ccaaaatcct
    10861 taacacaaac gttggccgag acaccgcagt ccgaaagcct gcgtactacc tcaataccaa
    10921 aggcatcgtc gccgaaaaag atattcccga tgcccccgac cagtatcttc atgtcccggc
    10981 caccttaggc gacagcagct cgatctcctc cgcacgaaag aaaaaccggt gcccaatctg
    11041 gcgcatttcg ccaaggtctc gccccggatc gtcgtcgatc gtcacggcaa cataaatttc
    11101 accttcgaat gtttgctcaa gagcttcgat cgttgcctct ttgccttgta gcgcaatatc
    11161 gaaaatgtcc gcgcggtttt gtggccacac tcgcacacga tcgccttttc gcagatccac
    11221 tccagatacg catattgttt ccagtcgcgc catgtcctca tcgctccact cgttcactgc
    11281 gttgttcttc ggcgcgcgaa acgctccatg catcccgcga atctctgact ccgaaaggtt
    11341 cgtcgtacga ttgagaatct ggcgagcgga atctggagct ctctgcacga tttcttcccg
    11401 ctcagcgtcg gaaagagtca gcacgcgcag cgcgagcatt tcgtcaattt ctgagctgtc
    11461 gaagaaatcg cctttgctct ccggggcgac ttgcggaaag tcatagagga tgatcggcga
    11521 aatcagcatc gtagctcgtg tctgaacgtc tccggccagc accggataga cgccgacttg
    11581 tttgcaccgt gcggcctctt cacgcaattc atccggcggt tcgagcgaag atgtgaattc
    11641 acccatatgg atcgtcagca gcccattcac gctaagtaac gaataattca gagcagcatc
    11701 gcggctagcg caagctccgt ccggcatccc actcatattg cgaacgcgga cggtgtatcg
    11761 gaagatattt cccgttgtat gctctgctcc aacctctgcg ctgccttgta tccggtctcc
    11821 ttcgaaaccg ggaaccggaa attcaaatga cgtttgtgaa ttctgtaaaa gcaagctctt
    11881 caactcggct tgcacgttta cacatcgctc gattacgcct ccggattcgg gtccgtcggc
    11941 atcgagctgc agaaaacgaa cctccagttc caacattgca tcaccggaag tgcgcgccag
    12001 cacttgcgct tgcatcaaat ccgattcgcc gggattctcc gcaacatatg cacgaggcag
    12061 tagagcccca aaggtccagc cgggccgctg gttcttgagc gcggatgcgc gatacggata
    12121 caacaagcat ccttcatata ggagagtgct cgaaagtttc cgtagctcgt ctaactgcat
    12181 cattgaaccc gtgtctccgc cgtatccccg gctgcggcta gcacgtgctc catcgcctgt
    12241 tcccacgtca gcaggccgtg ccgcgccttg tatcggcgta ggcgctcgaa aacgtctttt
    12301 cgcagactga gccatgccga attggggtaa tacaaggcca tcatttcttt ccacaccgcc
    12361 gcggcgattc gaaattctgc ctcgcgcgac caagaaaccg gcgccatctg cagcgcgcca
    12421 ttcgcaagat aaaacaccat gccgctgaat acccagacga ccgggatgtc gccctgctcc
    12481 agtgcggcgc aatattttgt cgctgcaatg ttaaaatcaa acgtgcacgg aacgcgtaca
    12541 tccaccactg tgtttgcaca aaacgccgga accgttaccg taacgcccgt ccaatagagc
    12601 cgacgaagcg ttttgcccca ccgttctctc tcgccaaaaa gatccgccaa tgcgctttgc
    12661 tcggccgctt cgtagcgccg ccgcgccgga tggatgtgca actggcagtt cagtgacacg
    12721 gagcgcacgt cctcatcttt caacgtattt tcgacgcgca gcgaaaatac gaggacgggc
    12781 gtagctgaat ccgacatcgc tttcacgtct tcgatcttaa acagcaggtc gggcatccat
    12841 cgctcccacg ccaatcgctt gttcagcgag cctcttaaaa aacttgtgca cttcggccca
    12901 gagttcatct ccacccgtaa aaccactcca atacttgcgc agcaaccccg tcaactcgta
    12961 gcagcgatcg atcggcgcga ggaaatatcg gcgggaatcg cctaagcggt ttaccagcag
    13021 agcctgcacg tccgactgca tctgttgcac tcgcggatgc cgctgcgtaa tctcttgcca
    13081 gacggcctgc ggcacttccg cctgcacggc tcctccagga ctcggataaa ccgctgtgac
    13141 cctcttcatt gcgctgctct caaagaagaa cgcaaggccg atcggaatcg acaggctctc
    13201 ccactcgaca tcgtcgatct cgaagtttcg aaggtgaaac gcgtctcgcg gaattcgcct
    13261 gtatcgcatg cttgtcgaac tctcaaagag aagggcgcaa gcatcgcacg tgcaaacaat
    13321 ccgccgctcc cttggatcga tcgcgtgtgg atgttcggct cctaaatcgc gggaacaaat
    13381 ctcgcaaatt tccgccgctt gcggcattct gcgtagccgc ttcagcgcag caaacccgcc
    13441 cgcgtcaaga ctcggttggt ccggcgcccc catctcaact cacctcagaa tctctatgcc
    13501 ggatgaatat gcggcattcg cggatcgata ggatcatgcg tcttcggatt gatcgacgtt
    13561 gaactccgtg ctgtcggaac atcgccctcg cgtcccgcct ccagtccgcc gctctgcaac
    13621 cgctcttctc cgcgcttcgt accttctgta tgcgaagaca gttgtgtctt ggatgtatat
    13681 gtctcgggca ttgctcactc cctctaagat ttcgcagccg ccggctcaaa agaaactgcc
    13741 gccggtcgga tcgaattgat cggcacaaac gtcttgtcca caacggccaa ctccaccgta
    13801 atttcagcgg cgtctggcgc aatgccgatc aaggaagctt cgacgaacga gcgcacagtc
    13861 tctgcggtcg atccacatcc gccttccatg ccggaaacgc gcactctcac ggcgttttca
    13921 tctgcttgaa tcacttccgc cacggcgccg aacttttcca tcttcggtcg cagctctgca
    13981 agcatttcca ctactcgctc accaatccct cttcggtcga ggtcgtgcag tagcaacacc
    14041 gaacgaacca actcgtcttg ttcaaacgcc gcgaatactg cggccgccga cccattctgt
    14101 ttcacaattg ccagcattcg ctccagtgca catcgatgca gctcaagcac ggacgcgact
    14161 aaatccgtcg ctgcggcgcg cgccgccgga tcggcaatcg agcctagtcg ctcaacctgc
    14221 cgctcgatgt gcagactttc gctcgcttga tcctcccgat ttgaactcat ccgccgtgat
    14281 ccatcagccc aaacatcggc acatgccgcg cttcgagcag tttgcctttc cccgtataca
    14341 tgtgaacgcc acacggcagg caagggtcaa agctccgcac cgcacgcatg atgtccacgc
    14401 ccttgaaatc cgcgggaccg ttctcctcga agatcggcat gtcctgcaca gcgtcctcgt
    14461 aaggcccggg cgtgccataa atatcccgtg gattcgcgtt ccaaggtgtt ggtggatagg
    14521 gatgatagtt cgcaatcctt ttattccgta tcaccacgtg atgcgaaagc acgcctcgga
    14581 ccgcctcatg gaacccgact ccgatcgctt cttccggcac ttcaaatgga gcccacgttt
    14641 tcgtgtgccc tttttgaact tcttcaatcg cgcgttcgac aaagtacatc gccgctgccg
    14701 ccgcgtacgc ttggaaatat gtgcgcgcac ggttccgctc gatcgcgttg ctccatttcg
    14761 gaatcgtcca ttcgtattct ttctccggaa gtgcgcttgt cttcggcagc gtgattttca
    14821 cgcttcggcc cgtcgccttc acgtacccga tatcgacctt gttcgagagt gccgtagacc
    14881 acagtcgtgc aatcgggccg ccgcccgtat caagagccaa atgttcgccc gttctcttca
    14941 gcagccagcg cggcgacatc acccagctat atcgtttgtt gaaatctcgc ttctgcggcc
    15001 acggcgtagt gctctgattc cagggatgcc gcttatcgat cgggttgccg atgggatctt
    15061 gtctaacgaa ggtttcctta tcttcccagt cctcgtaata cgagctaccc agcagaatgc
    15121 gcatattcaa gttgatgtcg atcagatcgt ttgtcaccaa ttcaccgtcg acgatcacgc
    15181 cgggggtgac gtacatgtcc ttaccccaac tgcccatgtt ttcgtacttg taatcgcacg
    15241 tgtcggggtt ctgccaggag ccccaacaac ccagtagcac gcgtctctgc cccacttttt
    15301 cgtatcccgg tagagcctca tagaaaaaat tgaaaaggtc gtcgtgcaga ggcagcgcct
    15361 ttttcatgta ctcgcaatat ttcataagcc gcacgaggaa atcggtgaag agctgaatcg
    15421 tcgccacagt gccggtgcca ccgggataga gagtggaagg atgcacatga cggccttcca
    15481 tcaggcagaa catttctctc gtcaaccggc tcatgtggag cgcttcgcga tagtactcgc
    15541 ccgtgaatgg gttcagcgct cgcatgatat cgccgatagt gcgatagcca tgatccttac
    15601 tgtgcggagc ctccgtgttg ttggcttttt ccagtacgcc gggatttgtc gccttcacca
    15661 tctgctcgca ataatcgacg cctaccagat tgtcctgaaa aatgttgtga tcgaacatat
    15721 actcggcggc ctcgccgaga ttgatgatcc actcgcccgt aggtggcgtt tttactccaa
    15781 acgccatgtt ctgtgcgtag acggcgcacg tcgcgtgatt gtccccgcag attccgcaga
    15841 tgcggctcgt aataaaagga gcgtcgcgcg gatctttgcc tcgcatgaag acgctgtatc
    15901 cgcgaaacat cgaagacgtg ctgtaacact ccgccacttc gcggttatta aaatcgatct
    15961 tcgtataaat gcccaggctg cctacgatcc gggtaatggg atcccagttc atgtcaacca
    16021 gcgtgcgatt ccctttctgg cccgccaaag aggtatcagt ggtgactgcc atttgctccg
    16081 tcctttctca ttccattccc gtaagtcgtg ggcacataac ctgtggagag agtcgttcct
    16141 ttgtggcgcc atttgggctc tcgattactg ctgttatttg tgatggaacg cagcgaacgg
    16201 atcatcgtgc cgtatatgcc taccgtgccc gcagacagtc tcgcgcctgg cggttcgtcc
    16261 ataaacggca tgaacttgtc tgggaatcct ggcatcgtgc agccgatgca aatgccgccg
    16321 acattcgggc agccgccaat gcctcgcatc caaccccgct tcgttacgtt gcagttgacc
    16381 actggacccc agcagccgat tttgaccagg cacttcggcg aaccgtattc atgcgcgaaa
    16441 tcgccttgct cgtaataacc tccgcgatcg catccttcat gcactgtctt cccgaatagc
    16501 catgttggtc gcaacatttc atcgagcggg atgatcggtg caagattcgc tgcctgaaaa
    16561 cataaataga gcagtgtctc agtcatgttg tccggctgca ccgggcaacc gggaacattg
    16621 acgatgggca gaccggccga tgatcgccaa tcccacccaa ggtagtccgc caagcccatc
    16681 gaaccggtcg ggttgccggc catcgcatga atgccgccgt atgtcgcaca agtccccgca
    16741 gccaccacgg cgagagcctt cggcgcaagc ctgtcgatcc aatcgcatag agtgattggc
    16801 tggccatccg gatcgttccc aatacctgtc caatagccct cgcgtttgat cttctcgttg
    16861 ggaatggagc cttcaaccac aagaagaaac ggcgacagct ctccgcgttc agccttataa
    16921 agccatgaca tgaactcttc gccgccctgt tccgctgcta ggactcggtt gtgcagaaca
    16981 accttgggga ccccaggaat agcaccgagg attaaatctt cgatggatgg ctgttctgcc
    17041 gcggtgactg aaatcgtatc gccatcgcag ctcatcccgt ccggtagcca aatcacatga
    17101 acttcagcag gtgcgggacc tttcggtttc aaggaactca aactcggaga ggccatgaca
    17161 tctcgctttc gcgcaaagtg tcac
//
LOCUS       DATNDT010000257         3052 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_914133, whole genome shotgun sequence.
ACCESSION   DATNDT010000257 DATNDT010000000
VERSION     DATNDT010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914133"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="VN788_08260"
     CDS             complement(<1..1574)
                     /locus_tag="VN788_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU20559.1"
                     /translation="MAERSARSSPPGSASDGKKQSVWAMLPDVWSLIKPRRAILALGL
                     ALMAVNRVSGLVAPISSKYLFDNVIAKHQGRLLPLIILVVLGATILQGLTSYSLTQLL
                     SKSSQRMITELRVKVQAHIGRLPVAFYDANKTGVLVSRIMSDVEGIRNLVGTGLVDFV
                     GGTMTALIALAYLLRVSVSMTVAAFVAVLIFGLGLGQALKKIRPMYRARPKLNAEVTG
                     RLTESLGGVRVVKGYHAEEREEKVFGLGVQKLLDNVLQTLTATSLMSFSATALMGIVT
                     ASMMYLGARNILAGTMTPGDLVSYIALLAMMIAPVAQIAAIGPQMSEAMAGLERTREV
                     LNEQVEDKDPRRTIRLDRIEGHVAFENVDFAYETGKQVLRSINFESAPGTMSAFVGPS
                     GAGKSTIIGLIAAFYVPTAGRVMVDGVDLSQVRLDSYRTQLGVVLQETFLFDGTIREN
                     VGFARPDATEEQILAACRIARVDEFAENFSDKYDTLVGERGVKLSGGQKQRVSIARAI
                     LADPRILILDEATSSL"
     gene            complement(1858..2214)
                     /locus_tag="VN788_08265"
     CDS             complement(1858..2214)
                     /locus_tag="VN788_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020676525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HXU20560.1"
                     /translation="MEQAAKHERFEGERTLMRIHIGESDRWHGKPLYEAIVGLLRQEK
                     FSGATVLRGVGGYGGASIYHTDKILRLSQDLPIVIEVVEFTDRIEQILPRLDEMIGGG
                     LVTLEKVRVILYRSHK"
     gene            complement(2233..2604)
                     /gene="crcB"
                     /locus_tag="VN788_08270"
     CDS             complement(2233..2604)
                     /gene="crcB"
                     /locus_tag="VN788_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXU20561.1"
                     /translation="MRIALLVLFGAAGTLARYAMQGLVQKRTGPEFPYGTLAVNIVGC
                     FLLGGLAEYGLTHLTIPPEWRIGITTGFLGAFTTFSAFTYETVRLFQDGQWFRAITYV
                     LASILIGILAVFSGIRIADRI"
     regulatory      complement(2674..2737)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            2868..>3052
                     /locus_tag="VN788_08275"
     CDS             2868..>3052
                     /locus_tag="VN788_08275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20562.1"
                     /translation="MAESILTFDFGTNEEGAQQARHKIEGWKQGFRLGNKMILKFERD
                     DGAEQTAESDGASANEES"
BASE COUNT          694 a          804 c          940 g          614 t
ORIGIN      
        1 tccaggctgg acgtggcctc gtcgagaatc aggatgcgcg ggtcggccag aattgcgcgg
       61 gcgatggata cgcgctgctt ttggcctccg gaaagcttta cgccgcgttc gccgaccaga
      121 gtgtcgtact tgtcgctgaa attttcggcg aactcgtcca cccgcgcgat gcggcaggcg
      181 gcaagaattt gctcttcggt ggcgtccggg cgggcgaagc cgacattttc gcggatggtg
      241 ccgtcgaaga gaaaagtctc ctgtagaacg actccgagct gggtgcgata ggaatccagc
      301 cgcacttggg aaagatcgac gccgtcgacc atgacgcggc ccgcggtggg aacgtaaaag
      361 gcggcgataa gcccgatgat tgtggacttg ccggcgcccg agggaccgac aaaggcgctc
      421 atcgtgccgg gcgcggattc aaaattgata ctgcggagca cctgcttgcc cgtttcgtac
      481 gcgaaatcca cattttcaaa agcgacgtgg ccttctatgc gatcgaggcg aatggtccgg
      541 cgaggatctt tatcctcgac ttgttcgtta agaacttcgc gagtgcgttc gagaccggcc
      601 atggcttcgg acatctgagg gccgatcgcc gcaatttgag cgacgggcgc gatcatcata
      661 gccagaagag cgatgtagga gacgagatcg ccgggggtca tcgtgcccgc gagaatattt
      721 cgcgcgccga gatacatcat cgaggctgtg acgatgccca tgagcgcggt ggctgaaaaa
      781 ctcatgaggg aggtggcggt gagggtctga agcacgttat cgagcagttt ctggacgccg
      841 aggccgaaga ctttttcttc gcgttcctcg gcgtgatagc ccttgactac gcgcacgccg
      901 ccgagagatt cagtgagcct gccagtgact tccgcattca gctttgggcg agcgcgatac
      961 atggggcgga tttttttgag cgcctgtccg aggcccagcc cgaagatcag gacggcgacg
     1021 aaagcggcaa ccgtcatcga cacgctgacg cgaaggagat aagcgagggc gatgagcgcg
     1081 gtcatggtgc cgccgacaaa atctaccaaa cctgtgccga cgagatttcg aataccttcc
     1141 acgtcgctca tgattcgaga aacgagaaca cctgttttgt ttgcgtcgta gaacgccact
     1201 ggcaggcgcc cgatgtgcgc ctgaaccttg acgcgcaact cggtgatcat tcgctgggag
     1261 gatttcgaga gcaattgcgt gagcgagtag gaagtcaatc cctgcaaaat ggtggcgccg
     1321 agaacgacca ggatgatgag cgggagcaga cgtccctgat gtttggcgat tacgttatcg
     1381 aacagatatt ttgacgaaat aggagcgacc aagccagaga cgcgattgac ggccatcaaa
     1441 gcgagtccga gggccagaat ggctcggcgc ggtttgatga gggaccaaac atctggaagc
     1501 atggcccaga cggactgctt cttgccatca ctggcggagc cgggaggcga ggaacgcgcg
     1561 gatcgttcgg ccatacgcgc gtggttgccg gatggacttc ctgggaatgg tggcatgcta
     1621 gctattctgg ctcggggcag aaatgcgtag tgcctgcgcg caccaaatcg tcctagtgaa
     1681 atcgaatgaa gatgcgaaag ggctcccgat catatttgat tatagcgaaa aggaagtggg
     1741 tagtgcgatg gagcgattct tcggcatgct tcagggagta ccggtcggtg ttggagacgc
     1801 gatttgcttg cgatttgccg gatcgcgcag acgaaaatgc ggcacgcggg ccgcagatta
     1861 tttgtgggat cgatagagaa tgacgcgaac tttttcgagg gtcaccaggc cgccgccgat
     1921 catttcgtcc aggcggggca gaatttgctc gatgcggtcg gtgaattcaa ccacctcgat
     1981 cacgataggc aagtcctgtg aaaggcgcag aattttgtcc gtgtgataaa tgctggcacc
     2041 tccgtaccct ccgacacccc ggagaacggt ggcgccgctg aatttttcct ggcgtaaaag
     2101 tccgacgatc gcctcgtaca gcggtttacc gtgccagcgg tcggattcgc caatatgaat
     2161 gcgcatgagc gtgcgctcac cttcaaagcg ctcgtgtttt gccgcttgtt ccatgcgacc
     2221 tccagaggcc gtttaaattc gatcggcaat gcggatgccc gaaaacacag ccagtattcc
     2281 gatgagtatg ctggccaaaa cataagttat ggcacgaaac cactggccat cctggaatag
     2341 gcggacagtc tcataggtga acgccgaaaa agtcgtaaaa gcaccaagga atccggtggt
     2401 aatgccgatt cgccattccg gggggatcgt gagatgggtc aggccgtatt cagcgagccc
     2461 gccaagcaaa aagcagccga cgatatttac cgccagcgta ccgtatggaa actcgggccc
     2521 cgtgcgtttt tgaaccagtc cctgcatggc gtaacgcgcg agcgtgccag ctgctccgaa
     2581 cagcaccaac agtgcaatcc tcacggctgc tcctcccgtt acgcaatctg atcgcgacga
     2641 agggccccta aaacaaaaac tcctgggcgc cgagggcggc caggagtcat cagcccgtta
     2701 tcgagcgatt gaggcgaact ccatcgcctg taaattttcg gcgcaaatcg taccacacaa
     2761 agagatgttt gcggtcgtct tgtgggtgct tgctccgggg ctagcgaatt ggcccttccg
     2821 gcgccgcagt tcttgacact cacccttcgt cccgctatag aagatacatg gccgaatcca
     2881 ttttgacttt tgactttgga acgaacgaag aaggcgctca gcaggctcgc cataaaatcg
     2941 agggatggaa gcagggcttt cggcttggca acaaaatgat attgaaattc gagcgcgacg
     3001 acggggcaga gcaaacggcg gagtctgacg gcgcatctgc caatgaagag tc
//
LOCUS       DATNDT010000258         9028 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4193606, whole genome shotgun sequence.
ACCESSION   DATNDT010000258 DATNDT010000000
VERSION     DATNDT010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9028
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4193606"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="VN788_08280"
     CDS             <1..865
                     /locus_tag="VN788_08280"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HXU20563.1"
                     /translation="RRMTQSGRSLDRRFIASLAHGLSVLEAVADSTNDIALADLARRV
                     GFKKTNTWRLVHTLVKLGYLHQDSRTRNFRPAPRILALGYAYFDGLDLKKLPLPLLHE
                     LAARHNETVNLAVQSGDELIYIERITTSQIVSINLHVGSRLPLYCTSLGRALICEMPS
                     AWLEQYIERIASDARARKYVQGGGKRLLEMLHETRERGYAVNDEELVKGLRAVASPIR
                     DRTSKIVAAVCISVPSSRRTIGDLRRVFAPDLVSVAADISLALGYRPKESTRISNGIR
                     NARSESLGRAS"
     gene            1055..3334
                     /locus_tag="VN788_08285"
     CDS             1055..3334
                     /locus_tag="VN788_08285"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU20564.1"
                     /translation="MRLGFRSSKWYFIVAVVVSIVSMSIGQKVLRAAARAKESPKTVE
                     WVNTSLDAAETRYSPLDQINTSNVSRLGLAWYYDTHSALGALEATPIVSNGIMYGTLS
                     WSMVFAVDAQTGKELWRFDPKLGHQNFPLGADGSPDPTKPRTGPSPCCGAVNRGVVVY
                     NGKVYIGTLDGRLIALDAKTGKIVWQVHTTDPDSDYTITGAPRIAGDKVIIGNGGGEY
                     ALRGYVTAYDAETGKQVWRFYTIPGDPSKPFENKAMAMAAKTWTGNVWYKMGGGGNAW
                     DAFAYDPDLDLLYFGTGNGGPYPQIVRSPEGGDNLFICSILAVRASTGEYVWHYQVVP
                     SDEWDYDSVQSLILANLDIEGKTRKVIMQASKDGYFYVLDRTNGKFISAVPIVKVTWT
                     TGIDPRTGRPNIPASSFYDRNSAGVWLTPSGGGVHNWHPMSFNPTTGLAYVPGGSGGS
                     EWFAVDPSFKFKEGNFNWAQLRNPPTMINGVRAIAKQNTPPPPGMNEADGEAIARPAG
                     AAAPAPAANISPLGRSFLVAWEPVTQKAAWSVPGLNGGGTMTTAGNLVFAESNDGHIV
                     ALSADKGNKLWEFKLTPGLASPMTYMIDGKQYVSILGGSTGNGRLFTFALDANTPLPP
                     PAPPAHGSSSPAYTEAQATRGKAAYAQSCAVCHGDTLAGNGENPALTGSTFSATWNGH
                     AAGDLFELIRTTMPKGSPNTLKPDVYADILAYIMQVNKMPAGRDELKSAPEALERVIM
                     SVGNGGGPRDAGQGLAAPR"
     gene            3537..3890
                     /locus_tag="VN788_08290"
     CDS             3537..3890
                     /locus_tag="VN788_08290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20565.1"
                     /translation="MNPQALPSEALIYDRYSLRSLSAKDFERIDRALVIHAYGVGFTI
                     TVRSISQGFRHVPVRVKLCVRRQRKTARWAEGVAVVRAENVKMAIRVPAGPLRRGVEA
                     VRGASDSGDFRKDNS"
     gene            complement(4033..7224)
                     /locus_tag="VN788_08295"
     CDS             complement(4033..7224)
                     /locus_tag="VN788_08295"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU20566.1"
                     /translation="MNSTTWNAAIARVVLSAFALLAVAIGAAAQSTVTINGTVKDQTG
                     APVADATVTATNVGTGISSKAPTSAEGLYVIAGLPAGFYNVEASQQGFSTVVRAHQEL
                     LVGTTVTLDFTLKVSSVAQTVEVQGVAPDVEPTESTVSAILHTQQLDDLPILSRNFSE
                     MASLTPGVQSTSQVSVGFAVGGAVTGVTIGNSTQYETGYTVDDIPITKPSDGGLYVAL
                     AQDWVQEFSVVSNQASAEYAGAVSGVVNAITRSGTNQVHGRAYSFFQNSDLNASNFNA
                     VTHVRKAPSSSQRVGGMLGGPIKKDKLFYFIGYEYYRSLAGVSFAGLPSTFAGSRDVM
                     PVDLVPSNPSNLNRQDLGEVKLNYHARPSDSFEFVGNIQRIGEPTQASATNAYASLLN
                     GNSTTNEDIGFSLNWTHIFSPTSINSLRVYNATDWDKETCNFAQAVGPFTTTNPALTN
                     FGITPNGNPTGFYAELTYTLPQGLADVGCAGRFGGKHFIGLYAGIYDTATKTKGRHEI
                     KFGGQAADPFYGSNKLHSNSDGFYGFPTANIAATSDGILTPYAAGVPFNPSDPSTFPR
                     TLTMNYGPFSHTYVMATGWSFGVFLEDNWRITDNFTLNWGIRWDGDLQYSHFNDNFAC
                     VTGTSHCLNPAVNNGNYVSNDFRTVSPRIGFAWTPFGRDKNTVLRGGFGAYYGPTTLT
                     EATGYISQRTNADIIDGFTSNSPTGNPYCAALGGCGTAATFERYQNDLRELLAWSLVN
                     NTIPDLTRTSVSVGGTTYPLPGVASFAPPLSNVYNIDPNMKKPAALQSTIGIAHSFNN
                     GLSFTVDYAYVYGFDQDIEREVNFNPNTIPVVTLLNPNFTTEYSTCSCGWFSDKQILA
                     KVRYNDHRGDTAQVSYTLGWAYSNSASTSDFNLHSVGAYNTDPFDFEVDRGPATTDQR
                     QILAVNGVVRLPGDIEFAPIVTVGTGLPMTATTTQAPGAAPIHVSNGNVITVPGCQPW
                     YNQCYPAINGVQTRKGFYRGATTVGVNARIQRPFKLRENMSITPMFEAYDIPNLVNQG
                     TNYTLAATSPSFLKPSGATITMRQLQLGVRFDF"
     gene            complement(7511..7777)
                     /locus_tag="VN788_08300"
     CDS             complement(7511..7777)
                     /locus_tag="VN788_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018671406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HXU20567.1"
                     /translation="MKLYAAIQTVLDAAKGESYRQAHVEFLERLVADGRIFARGRFPD
                     GSGGLTIFEAPSLEDARKAAESDPYVIHGVRRLEIHEWDMKIKH"
     gene            complement(7886..>9028)
                     /locus_tag="VN788_08305"
     CDS             complement(7886..>9028)
                     /locus_tag="VN788_08305"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU20568.1"
                     /translation="GLGAGMFFVSYSAFALPSNLLLTKIGARKMIFGSLFGWGLTSSA
                     TMFIHSPMQFYALRFLLGVFEAGFFPGIIYYFGEWYPSDRRASVIGIFTSATVVAGVA
                     SGLLSGSIMTYLNGFSGLQGWQWMFLIEGIPSAVIGIGVYFYLDNKPQEVKWLSDVEK
                     KALLSALKDDPVVGSTNRTLGRALSDWRVYLLGVIYFLAVFDTFVLAFWQPAMIKAFG
                     VSSLMAIGIYSTIPPIAAIIAKVYVGYHSDLKKELRWHFAIPAFLGAIGFSSMTLLSH
                     NPLWGIACLTLATAGVHGCMPIIWAAPGQYLSGTGAAAGIAVISTMGTTSGAVGPALL
                     GLIRTATGSFSLGMYVLSAILSLAAILMLVLVKKRNRVPAAENSPA"
BASE COUNT         2036 a         2525 c         2512 g         1955 t
ORIGIN      
        1 gcgacgtatg acccaatcag gtcgatcctt ggatcgtagg ttcatcgcct cgcttgcaca
       61 tgggctctcg gtgttggaag ctgtcgccga cagcacgaac gacatcgcac tggctgactt
      121 ggcaaggcgg gtgggcttca agaagactaa cacctggcgc cttgtacaca cgctggtcaa
      181 gctgggctac ttgcatcaag actctcgcac acgaaatttt aggccagcac cccgcatctt
      241 ggccttgggc tatgcatatt tcgatggact cgacctaaag aaactgccac ttccattgct
      301 gcacgagctt gcggctcgcc acaacgaaac tgtaaatttg gcagttcaga gtggcgatga
      361 attgatttac atcgagcgca ttaccacttc gcaaatcgtc tcaatcaatc tgcacgttgg
      421 ctcgcgtctt ccgttgtact gcacgtccct gggacgtgcg ttgatctgcg aaatgccgag
      481 tgcgtggtta gagcagtata tcgaacggat tgccagcgat gcgagggctc gcaagtacgt
      541 acaaggcggt ggcaagaggc tccttgagat gcttcacgaa actcgcgaac gcggatacgc
      601 ggtcaatgat gaagaacttg ttaaaggatt gcgagccgtc gccagtccta ttcgcgaccg
      661 tacttccaag atcgtagcgg cggtctgcat ctcggtacct tcgagccgaa ggactatcgg
      721 cgatctgcgg cgcgttttcg caccggacct agtgagcgta gccgccgaca tatcgctcgc
      781 gctcggctat cgcccaaagg agtcgacccg gatatccaac ggtattcgga atgcaaggag
      841 tgagtctctc ggacgcgcta gctagacgct acctgcattt tagacagcct gctatccgac
      901 aagccatcct cgataaatgg gtccgcatag caaccttcat ctcgactgag atcgtcggtg
      961 cgtcgtgaga ttattacaag cctgcgccgt gtaatgctgt tttggaagtg tttggccaac
     1021 ctggtaacgc catacggctt ttgaggattt ttatatgcga ctcggcttta gaagttcgaa
     1081 gtggtacttc attgtcgcgg tggtggtgtc aatcgtttca atgtccatcg gccaaaaggt
     1141 tctgcgcgcc gccgccagag caaaagagtc ccccaaaacc gtcgaatggg taaacactag
     1201 cttagacgcc gcggaaacgc gatacagtcc gctcgatcag atcaatacaa gcaatgtaag
     1261 tcggcttggt cttgcctggt attacgatac gcactctgca ctcggagcgt tggaagccac
     1321 cccgatcgtc tccaacggaa tcatgtacgg aacgctgagt tggagcatgg tctttgccgt
     1381 ggatgcgcaa accggaaaag agctctggcg cttcgatcca aagctgggcc accagaattt
     1441 ccccctcgga gcggatggca gccccgatcc cacgaaaccg cgcaccggtc ccagcccgtg
     1501 ctgcggggcc gtgaaccgcg gtgttgtcgt gtataacgga aaagtctata tcgggacact
     1561 ggacggacga ttgattgcgc tcgatgcgaa gaccggaaag attgtgtggc aggtgcatac
     1621 caccgatccc gactccgact acaccatcac gggcgcaccg cggatcgcgg gagataaggt
     1681 gatcatcggt aacggcggcg gtgaatatgc actccgtggc tacgtgacgg catacgatgc
     1741 cgagacaggt aaacaggtat ggcgcttcta caccattccc ggcgatccat cgaagccgtt
     1801 tgaaaataaa gcgatggcga tggccgcaaa aacctggaca ggaaacgtat ggtacaaaat
     1861 gggcggcggc ggaaacgcct gggatgcatt cgcctacgat ccagatctcg atctcctcta
     1921 cttcggcact ggcaacggcg gtccctatcc tcagatcgtt cgcagtccgg aaggtggcga
     1981 caacttgttc atctgctcga ttctcgccgt tcgcgcgagc acgggcgaat acgtctggca
     2041 ctatcaggtc gtgccctccg atgaatggga ctacgactcc gtgcagagcc tgatcctcgc
     2101 gaatctcgat attgaaggaa aaacccgcaa agtcatcatg caggcctcca aagacggcta
     2161 tttctacgtg cttgatcgga caaatggaaa attcatctct gcggttccga tcgtaaaagt
     2221 aacttggacg accggcatcg acccgaggac ggggcggcct aacattcccg cgtcctcttt
     2281 ctacgatcgc aatagcgccg gtgtatggct tactccaagc ggcggtggtg tccataactg
     2341 gcacccaatg tcattcaatc cgaccactgg actcgcctat gttccgggcg gctccggggg
     2401 ctccgagtgg tttgccgtgg atccgagttt caaattcaaa gagggcaatt tcaactgggc
     2461 tcagcttcgc aatcctccga cgatgatcaa tggggtacgc gcaattgcta agcagaatac
     2521 tccgcctccg cccggtatga acgaagcaga tggcgaagca atcgctcgtc ctgcgggtgc
     2581 ggccgctccc gctccggctg ccaacatctc gccgcttggc cgcagctttc tagttgcgtg
     2641 ggagccggtc actcagaagg cggcgtggag cgtgcccgga ctcaatgggg gcggcacgat
     2701 gacgactgcg gggaatcttg tttttgccga atcgaatgac ggccatatcg tcgctctcag
     2761 cgcggacaaa ggtaataaac tgtgggaatt caagctcacc ccaggattag caagtcccat
     2821 gacttacatg attgatggca agcaatatgt ttcgattctc ggcggcagca cgggtaacgg
     2881 gcgtctcttc acatttgcac tcgacgccaa tacccccctc cccccaccgg cgcctccggc
     2941 ccacggcagc agctctccgg catatacgga agcacaagcc actcgaggta aagcggcata
     3001 cgcgcagagc tgcgctgtct gccatggaga tactctcgcc ggcaatggcg agaatcccgc
     3061 gttaacgggc agcacgttct cggccacatg gaacggccac gccgcaggcg acctcttcga
     3121 gctcatccgc acaaccatgc ccaagggcag tcccaatact ctgaaaccgg acgtttacgc
     3181 agatattctc gcctacataa tgcaggtcaa taaaatgccg gccggccgcg acgaactgaa
     3241 aagcgctcct gaggcgcttg agcgggtgat catgagcgtg ggcaacggag gcggcccgcg
     3301 tgacgcaggc cagggactcg cggcgccccg ctgaaatcgg agcggaatag cagacaagat
     3361 ctgttgtcaa ggctttgaaa gtacccagct caggagagtg gtcgtagctg cagtcgcgcc
     3421 gagaacacac cgctgcagaa gggcggatct gagtctggac tagaggcagc atcggcttat
     3481 cgagcagcct gttggtatta gctgaactta ctctcgccgc gacgaggcct cggctcttga
     3541 atcctcaagc gctgccgagt gaagctctca tttacgaccg ctactccctt cgctcgcttt
     3601 cggccaaaga cttcgagcga atcgatcgtg cattggtgat ccatgcatat ggggtgggat
     3661 ttacgatcac cgtgcgttcg atttcccaag gtttccgcca tgttccggta cgtgtcaaac
     3721 tttgcgtccg gagacagcgt aaaaccgcgc gctgggctga gggggtagcg gtcgtgcggg
     3781 ccgaaaatgt aaaaatggca atccgtgttc cggccggtcc tctccggcgc ggcgttgaag
     3841 cagttcgtgg agcttctgat agcggagatt ttcgtaaaga taactcatga actggcatga
     3901 agcgtcctct cgcgcatctc gagttacctg ccttccataa tgcccgttac gtctgtcgat
     3961 agcgcagagc ccgcgtctcg ctgcgctctt ttttttctca agtcggagtg gcacggggcc
     4021 atccggcagc ttctaaaagt cgaagcgcac gccgagctgt aattggcgca ttgtgatcgt
     4081 cgcgccgctc ggcttcagga aactaggcga agtcgccgcg agcgtatagt tggttccttg
     4141 gtttacgagg ttgggaatat cgtacgcctc gaacatcgga gtaatggaca tattctcacg
     4201 caatttgaat ggcctctgga tgcgagcgtt cacgccgacg gtcgtggcgc cccggtagaa
     4261 gcccttcctt gtctgcacgc cgttgatagc cgggtagcac tggttgtacc aaggctggca
     4321 accgggcaca gttatcacgt ttccattgct aacgtgtatc ggcgccgcgc ccggagcctg
     4381 ggtagtcgtc gccgtcatcg gcagacctgt gcccaccgta acgataggcg cgaattcaat
     4441 gtctccaggc agccgcacca cgccattcac cgccagaatt tggcgctgat cggtagtagc
     4501 aggcccccta tccacctcga aatcaaacgg gtcagtgtta tatgccccta cagaatggag
     4561 gttgaagtcg gacgtcgaag cagaattgct ataggcccat ccaagcgtgt aggacacctg
     4621 cgcggtatca ccgcggtgat cgttatatcg aactttggcc aagatttgtt tatccgagaa
     4681 ccagccacag ctgcaggtcg agtactctgt cgtgaaattt ggatttagta aggtgacaac
     4741 tggaatggta ttggggttga agttgacttc gcgttcgatg tcctgatcga aaccgtacac
     4801 gtatgcgtaa tcaacagtga aagatagtcc gttgttgaaa ctgtgcgcta tgccgattgt
     4861 cgactgcagc gcagcaggct ttttcatgtt tggatcaatg ttatacacgt tgctgagcgg
     4921 tggcgcgaac gaggcgacgc cgggcaatgg gtacgtcgtg ccgccgacgg aaaccgacgt
     4981 tcgcgtgagg tccggaatag tgttgttcac gagcgaccaa gccaaaagtt ccctaaggtc
     5041 gttctggtag cgttcgaaag tggccgcggt gccgcagccc ccaagcgcgg cgcagtaggg
     5101 atttccggtg ggtgagttag atgtgaaacc gtcaatgata tcggcattgg tgcgttggga
     5161 tatgtagccg gttgcctcag ttaaggtggt cggcccatag taggcgccaa acccgcccct
     5221 taagacggtg ttcttatccc taccaaatgg cgtccaggca aaaccgatcc gcggagacac
     5281 cgttcggaaa tcgttgctga cgtaattacc gttatttacg gcgggattca gacaatggct
     5341 ggttccagtg acgcatgcaa aattatcatt aaagtggcta tattgcaggt cgccgtccca
     5401 acggatgccc caattgaggg taaagttatc ggtaattctc cagttgtcct ccaagaacac
     5461 cccaaacgac cagccggtcg ccattacata ggtatgagaa aacggcccat agttcatagt
     5521 cagcgtccga gggaacgttg acggatcgct ggggttgaag ggaacacccg ccgcgtacgg
     5581 tgtcaatatt ccgtcagaag ttgcagctat gtttgcagtc gggaacccat agaagccgtc
     5641 cgaattacta tgtagcttgt ttgagccata aaaagggtct gccgcctgcc ctccaaattt
     5701 gatttcatgt cttcccttgg tcttggttgc cgtgtcgtaa atgcccgcgt agaggccaat
     5761 gaaatgcttg ccgccgaatc tgccggcaca gcccacgtcc gccaggcctt gcggcaaggt
     5821 ataggtgagc tccgcataga atccagttgg atttccgttt ggcgtaattc caaaattggt
     5881 tagcgcagga ttggtagtgg tgaaaggtcc taccgcctgt gcaaagttac acgtttcctt
     5941 gtcccagtcc gtcgcgttgt agactctcag cgagttaatc gatgtcggag aaaatatgtg
     6001 tgtccaattt agtgaaaatc cgatgtcttc gttggtcgtg ctattcccgt tcaatagcga
     6061 cgcgtaagca ttcgtcgctg aagcctgggt aggctcgccg attctctgaa tattcccgac
     6121 aaactcaaac gaatccgacg gcctcgcgtg ataatttagc tttacttcac ccaggtcctg
     6181 gcggttcaaa ttcgaaggat ttgatggcac taagtcgaca ggcatcacgt ctctgctacc
     6241 ggcaaatgtg gacggaagac ccgcgaatga aacgccggcc aacgagcggt aatattcgta
     6301 cccgataaaa tagaagagtt tgtctttctt gatcggcccg cccaacatgc cgccaacgcg
     6361 ctgcgagctc gaaggcgcct tccttacatg cgtgactgcg ttaaagttgg aggcgttcag
     6421 gtcgctgttt tggaaaaagc tgtaggctcg cccgtggacc tgattggttc ctgatcgcgt
     6481 gatggcattc accacgccgc taacggcccc tgcgtattcg gccgaagcct gattcgaaac
     6541 aaccgaaaac tcctgaaccc aatcctgcgc cagcgccacg tagagcccgc cgtccgaggg
     6601 cttcgtaatt ggaatgtcgt cgaccgtgta gccggtctcg tattgcgtgg agttacctat
     6661 cgtaacgccc gtgaccgcgc cgcccacggc gaatccgaca gaaacttggc tcgtggattg
     6721 aactccggga gtcaaggatg ccatttccga gaagttgcgg ctgagaatcg gcaggtcatc
     6781 aagttgttga gtgtgcagaa tggctgacac ggtgctttcc gtgggttcga catcaggcgc
     6841 cactccttgg acctccaccg tttgcgcgac cgaggaaacc tttaacgtaa aatcaagcgt
     6901 aactgttgtg cctacgagta attcctggtg ggcccgcaca actgtcgaaa agccttgttg
     6961 cgacgcttca acgttataga agcctgcagg gaggcctgcg atgacataga ggccctcggc
     7021 gctggtaggg gccttggaac taatccctgt tcctacgttc gtcgccgtca ccgtggcgtc
     7081 cgccacgggc gcgcccgttt ggtccttaac agtaccgttg atagtcacgg tgctctgagc
     7141 agctgcaccg atcgcaacag ctaaaagagc gaacgcactc agtacgaccc tcgcaatcgc
     7201 cgcgttccac gtcgtcgaat tcatcagtct tctccccttg aatctctgcg tttcagagcg
     7261 atcgctcggc cctattgcat ggcggagcta aagtgggaaa aaagtcagcg cgaagtgcgc
     7321 cacgttgtca ttcctcatta aggaacgata ttcccacctc ttggttgcgc ctacttaact
     7381 accgaggcca gggactgtca agcagaatct aacgtccttt cgtcgataag cttcaaggac
     7441 tacgtccgcg gcgcttgggc gttgcgcctc agggtagggc aaaggggagt ttcaagcgat
     7501 cgcgactcga ctagtgcttg atcttcatgt cccactcatg aatctcgagt cgccgcacac
     7561 cgtggatcac ataggggtca ctctcagctg ctttccgggc gtcctcgagc gaaggcgctt
     7621 cgaagatcgt caggccgcct gaaccgtccg gaaagcggcc tcgcgcaaaa atgcgcccgt
     7681 cggccaccag cctttcgaga aactctacgt gtgcctggcg atacgattca cctttcgcgg
     7741 catccagcac ggtttgtatt gcagcgtaca atttcatatg agcctcctca tgaaaagctc
     7801 aggactttcc gatgaccgcg cctcgaatgc gttcgaccat ccgcctggcc ttgccggctt
     7861 gcgagcacgg gcccgatctc gatgattacg cgggcgaatt ctccgcggca ggcacgcggt
     7921 tccgtttctt cacgagcaca agcattagta tcgcggcgag agacaagatc gcgctcagca
     7981 cgtacatgcc taatgaaaag ctgcccgtag cggtcctaat cagccccagc agagcgggac
     8041 caacagcgcc agaggtcgtt cccatcgtgc taatcacagc gattcccgct gctgcgcccg
     8101 tgcctgacaa atactgtccc ggtgccgccc aaatgatggg catacaaccg tgcacgccag
     8161 cggtcgcgag tgtaaggcag gcgatgcccc ataagggatt gtgggacaat agcgtcatcg
     8221 acgaaaaccc gatcgcccct aggaaagcgg gtatggcgaa atgccaacga agttccttct
     8281 tgagatcgga gtgatagcca acatagactt tcgctatgat ggcggcaatc ggcggaatcg
     8341 tcgaatagat gccgatcgcc atcaggcttg ataccccgaa cgccttgatc atcgctggct
     8401 gccagaaggc aagaacgaac gtgtcgaaca ccgccaggaa gtagatcact ccgagcaagt
     8461 aaacgcgcca gtcgctcaaa gcccggccaa gcgtccgatt cgtcgagccg acaactgggt
     8521 cgtccttgag cgcgctgagg agagcttttt tttcgacatc ggacaaccac ttgacttctt
     8581 gtggtttgtt atccaggtag aagtaaacac cgataccgat taccgcgctg gggattccct
     8641 cgattaaaaa catccattgc cagccttgga ggccggaaaa cccgttcagg tacgtcatga
     8701 tcgagcccga caggaggccc gaggcgaccc ccgccacaac cgtcgccgag gtgaagatgc
     8761 caatgacgct tgcccggcga tccgatgggt accattcacc aaaataatag ataattcccg
     8821 gaaaaaagcc cgcctcgaaa acccccagca aaaaacgcag cgcataaaac tgcatcggac
     8881 tgtggatgaa catcgtggcg gaggaagtga ggccccatcc gaacaagctg ccgaagatca
     8941 tctttcgcgc gccgatcttg gtcaacagga ggttgctagg cagagcgaag gccgagtagc
     9001 ttacaaaaaa catcccggcc cctaagcc
//
LOCUS       DATNDT010000259        14252 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2196519, whole genome shotgun sequence.
ACCESSION   DATNDT010000259 DATNDT010000000
VERSION     DATNDT010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2196519"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="VN788_08310"
     CDS             complement(<1..1159)
                     /locus_tag="VN788_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HXU20569.1"
                     /translation="MENRKWARAAKASCAAASGPFATFCILFSVLLFSFLIAGCASPS
                     EPYERRPPIPQAVSDLTATRSGNDVVLSFTLPNEAMDHRPLSAPLAFEIYRDFEPTPA
                     SGNATLQAPDNPTLRITVPASMAGRYSDQGRVRYADALSAEDFAQHAGNVAVYIVRTL
                     ASPKKSSANSNAAVISVQPAPQAIDDLKTDVTPSAIVITWTAPKALVDSTPIIAGYRI
                     YRAEAPSTATTQNPLLKSPLAQIGETPPDSQSFRDSQFNFGATYAYSVRSFAQYPNGT
                     LESTDSNLVILTPRDTFPPAAPQGLIVVPVPSQGNVPAHIELSWAISPETDLSGYNVY
                     RTEQASVPPKRLNTELLLTPAFRDMNVLSGHVYSYTVTAVDSSGNESGSSAA"
     gene            complement(1168..2574)
                     /gene="hslU"
                     /locus_tag="VN788_08315"
     CDS             complement(1168..2574)
                     /gene="hslU"
                     /locus_tag="VN788_08315"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HXU20570.1"
                     /translation="MVIYLTGEQQPAEAEALPSFDELTPREIVVELDKYIVGQNAAKK
                     AVAIALRNRVRRQKLQPELAEEILPKNILMIGPTGVGKTEIARRLARLAGCPFVKVEA
                     SKYTEVGYVGRDVESMVRDLVETSIDMVREEKLDEVAERAEQSAEERLLDLLLPSSPM
                     PSDAGPAAEAQRREQAQRTRDKMRAQLREGKLDQRLVEVEVRERSMPSFEIITNQGVE
                     EMDINVKDMLSGMFGQQKKKRKMTVSEAFDYLIQEEENRLIDMDQVTRIAVDRVEQMG
                     ILFIDEVDKIAGRESGHGPDVSREGVQRDILPIVEGTTVNTRYGMVRTDHILFIAAGA
                     FHTAKPSDLIPELQGRFPIRVELGSLSEADFVRILTEPKNALIKQYIALLDTEGLKLS
                     FTEDAVASIARFAARVNEQTENIGARRLHTILEKVLEEISFEAPEMKKKTVKLDAPYI
                     QKQLADIVKDQDLSRYIL"
     gene            complement(2598..3149)
                     /gene="hslV"
                     /locus_tag="VN788_08320"
     CDS             complement(2598..3149)
                     /gene="hslV"
                     /locus_tag="VN788_08320"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HXU20571.1"
                     /translation="MQISKKSAQIRSTTVLCVRKDTKVVLAADGQVTLGEGVIKHSAK
                     KTRRLYSDKILAGFAGSTADALSLFARFENKLQEYHGNLARAAVELAKEWRTDRSLRH
                     LDALLIVADAKGTFLLSGNGDVIEPDDGICAIGSGGPYALAAARALAKHTKMTAKDIA
                     QEAMRIASDICIFTNANFSIEEL"
     gene            complement(3250..4161)
                     /locus_tag="VN788_08325"
     CDS             complement(3250..4161)
                     /locus_tag="VN788_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20572.1"
                     /translation="MRIRKAAIATAVMLMMAWTTAPPKASAQDDQFLMPEQSAAKAKQ
                     ILAEAIQALGGEAYLNVRDVTCTGRLGQFDHSGELTGFGHFIDYQKPPLKERQENLPK
                     RNIISVYNGDQGWELDRGGVSNAPRADMEDFKEGAKRDIDNILRHRIHEAGMTLRYGG
                     SDTVDLRQAKWVELVDSDNQTIRIAFSDKTHLPIREVAETRDPKTQTKSEEIDYFANY
                     HPQDGIQTALQLTRERNGIKLFQAFFDKCDYNTGLSDSLFTKESLDERWAKVGKKQRE
                     KEAKQKKKDEKQDEKAGRSSSSSSSSE"
     gene            complement(4334..5089)
                     /locus_tag="VN788_08330"
     CDS             complement(4334..5089)
                     /locus_tag="VN788_08330"
                     /inference="COORDINATES: protein motif:HMM:NF019342.4"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HXU20573.1"
                     /translation="MDHKNHAPRIGVPYRTREEEVSRGTNKIEKYLAAVRRAGGQPVP
                     VSLAASHDAFAALVSSLDGIVLTGSPADVEPSQYGAPRHAKCAEADDNRERIDFALLE
                     NCFAARKPVLAICYGIQSLNVFLSGTLIQDVPSEIGTKIVHSDSDDGALDAHHVITVE
                     SESRISEMAGARETIVNSSHHQAVLMPGRDLRVTARAPDGVIEAVEWTGDENWVVGVQ
                     WHPERMMESDPLTLSLFGSLVKEAAIRKAPAIV"
     gene            complement(5131..6459)
                     /locus_tag="VN788_08335"
     CDS             complement(5131..6459)
                     /locus_tag="VN788_08335"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HXU20574.1"
                     /translation="MTYDESVRALLALGRELASPQQAHVHKFGLENIAALSEALGNPH
                     LRIPCVHIAGTNGKGSTAAMLDSILRAAGIRSGLYTSPHLERINERIRIDGADISDEA
                     FAAAWTRVRAAIEGLLASGKLSAHPTYFECITALAFVAFAKSGVEFAVYEVGLGGRLD
                     STNIVRPEVAIITSIDFDHESFLGHSITEIAGEKAGIIKAGATVVSSVERPEARAVIA
                     QRCEEMRVRLVEIDEAWRIDSVEDSGGLYRANVMTTGSQERFSIAPPLAGRFQIRNAL
                     AAVTAARLLATRGFSITQEAIERGIRAARWPGRLERLSERPAIFLDGTHNPAGAKELL
                     KFWEENFAGRRVVLVYGAMRDKAVDEIAGLLFPAADFVVLTEPMQPRAISAPVLGEMT
                     AHLARKATVIADPAKALEHAIEMASPNDAIFATGSLYLVGDLRAYWAGRR"
     gene            complement(6456..7313)
                     /gene="accD"
                     /locus_tag="VN788_08340"
     CDS             complement(6456..7313)
                     /gene="accD"
                     /locus_tag="VN788_08340"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXU20575.1"
                     /translation="MTWFKREKSAIENPTPVDERRVRTEGLWIKCSGCRAIIWKKDLE
                     ANWEVCPKCDHHFRLSARRRLELLLDDAQWTEHDAELASSDPLHFTDTKPYSQRLAEA
                     QEKLGMKDAILTVQGMLCGRPVVCCSMEFGFIGGSMGAVVGEKVARAIERSMAERQPL
                     IIVSCSAGARMMEGAVSLMQLAKVASALARLDDSRVPFISVLTDPTTGGVSASYAMLG
                     DLNIAEPGAMIGFAGPRVIEQTIRQKLPEGFQTAEFLLEHGFLDAIVHRKELKSYIAQ
                     ALRFFLDSP"
     gene            complement(7344..7880)
                     /gene="cobO"
                     /locus_tag="VN788_08345"
     CDS             complement(7344..7880)
                     /gene="cobO"
                     /locus_tag="VN788_08345"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015049513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HXU20576.1"
                     /translation="MPEGKGLLIVYTGPGKGKTTCALGTSFRAVGQGLRVLMVQFIKG
                     SWHYGELDAAKMLGDDRFEIRPMGRGFVKVGGAETDPEDLRLCEEAWNYGREQIYGEK
                     YDLVVLDEINYVISYKMLDPERVVESLTQRPERVHVICTGRNAHPKLVEAADLVTEMR
                     EVKHPYTKGVLAQRGIDY"
     gene            8048..9061
                     /locus_tag="VN788_08350"
     CDS             8048..9061
                     /locus_tag="VN788_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20577.1"
                     /translation="MRNRWMKRALWIVLPVMTIGAILLNVGAPAGAAETPPLAADACD
                     YGCLTAIVNQYLKALVAHDPRQIPVASGVKFTENTIPLKLGDALWGTISGLGTYKLYF
                     ADPQDGTAGIEASIRENGTPAILLLRLKVVKRKISEIEMLVHRNAQDATALEKFGHPN
                     AVWSQPLKPSQRVSRQEMVKIANSYFDGILHSAGDSVPFDSRCNRILDGSQDTNNPTA
                     KGWFDKDSFKPDAMGIRENMNTHIWSYIHSIDPRRIVVVDQKMGIVVGMFMFNHPGNV
                     KFADVPGVGKVPMPPVTQRPSSVLMGEFFKLEGGKIRQIEGISVALPYGAKTGWETAA
                     SKN"
     gene            complement(9145..9858)
                     /locus_tag="VN788_08355"
     CDS             complement(9145..9858)
                     /locus_tag="VN788_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXU20578.1"
                     /translation="MFNELIVSGQTGEMARTHKPWSIGLSVILQAGILGALLLIPLIY
                     TQVLPKTMLTTFLVAPPPPPPPPPPQQAVVKQVKPVKVIAVNKMAAPTVIPKKIEIAK
                     DEAPPAVNPNDMPGVAGGTGDVLGGVGTGAAPAPPPPAAPKRIHVGGNVQAASLVNKV
                     EPQYPAIAKTAHVSGTVVLHAIIAKDGSVEKLQFVSGPALLMASAMSAVKEWRYRPTM
                     LNGQPVEVDTTVQVVFSLG"
     gene            complement(10185..11048)
                     /locus_tag="VN788_08360"
     CDS             complement(10185..11048)
                     /locus_tag="VN788_08360"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HXU20579.1"
                     /translation="MTEIAAAPSVADMLRGFSIEVNPGESKIIDAAPNRLEPGTEVFM
                     TWIPGKNPLDTVGPAGKLRSAGLFPTPHIGARHVESAAQLEEFVRQLVAVGVDRVLII
                     AGDRDEPAGPFDSSLAVMQSGILQKAGITRISVGGFPEGNPKISEPLLWEAIAAKTKF
                     ARENSLNLTIVTQFAFASEPVVEWLRKVRAQGIDNPIRLGFAGPASLISLTRYALVCG
                     VGASMKVLTEKPQFAKLLIDKGPEPLIRGVAEMLGPPNGSALPLGVVGVHFFVFGGFN
                     KTVDWINTARA"
     gene            complement(11176..12411)
                     /locus_tag="VN788_08365"
     CDS             complement(11176..12411)
                     /locus_tag="VN788_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXU20580.1"
                     /translation="MRKLLGITLGIMTALGGFVDLGQIVFTTQAGALFGYKLLWSIVL
                     GTVGIIIYMEMCGRVAVVAREPVFSVVRARLGYNLGLVTLIASNLLNLITCAAEIGGL
                     AILLHLLTGWPERAVLIGSCIALGAIIHLSSFQWIERSFGLSGLLMIVFGVSAAALHP
                     NWSHLLAGLNPAPHVADGKQVLLYAYFAVGIFSAMLMEYEVHFYSTGAIEEDWKPQDL
                     GENFMVASLGSILGSVLTVGLMVLGALVFLPHRIFPQMLSSSIMAGAFPFAGKALTVA
                     VIGAMACIGGAAIETGLSGAYNVCQFFNFKWGKNRRAKQVPVFTVGWLGMLVFALIID
                     FTGMRPLTLVNIAIIFGMVVMPFTYYPILRAGADKKIMGTHANSRFVTILGTIFLALI
                     VAAAIAAIPLLWLTHGGKP"
     gene            complement(12408..12827)
                     /locus_tag="VN788_08370"
     CDS             complement(12408..12827)
                     /locus_tag="VN788_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20581.1"
                     /translation="MVRKQAFLVLTVVALESCGRAKTLSSAELRSNLASSISLASETD
                     IFAGQIEGGRLLPAFQSGHAAYLRDEAKREAKGLRKSSPQPSQLQVVRLCIEQLDLLS
                     RELALISEKQGHGKDFPAIRQRVQAILKSLTAAKAGL"
     gene            12997..13398
                     /locus_tag="VN788_08375"
     CDS             12997..13398
                     /locus_tag="VN788_08375"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXU20582.1"
                     /translation="MSRVLNYSLVAVAALMMASSAFAHHSHAMFDTDKKVTLAGTVKE
                     FQWSNPHCWIQVLVPDPKDPKAPPVEWGVEMGAPIQLIRHGWKAGSLRPGDKITVVIN
                     PLRDGRPGGQIVSAIGPDGKPIGAVPETPAP"
     gene            13588..>14252
                     /locus_tag="VN788_08380"
     CDS             13588..>14252
                     /locus_tag="VN788_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018059239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20583.1"
                     /translation="MIRRITIAAMLVAGCFYISQAQSSSNFSNSKARVEAFAKLPDWS
                     GLWEFDIWTDELDGQQMGPIGMQRAKIYAAQMQATFTPAWQAKLAQLKKTADAAKAKD
                     GSEPPAFEEFGQFGGCGWTPGFPATMLPGTYEWRVTPEEATLISLLGSVRHIYTDGRP
                     HPSKDELWPMSQGDSIGHWEGDTLVVDTVSIRSPVYFPGLARGVGVPFIPMSNELHTT
                     ERIR"
BASE COUNT         2978 a         4178 c         4054 g         3042 t
ORIGIN      
        1 cagccgcgct ggaaccgctc tcgtttccag aactatcaac ggcagttaca gtataggagt
       61 aaacgtggcc ggacaggaca ttcatatcgc gaaaagccgg cgtaagcaac agttccgtgt
      121 tcaaccgctt tggcgggaca ctggcctgct cggttctata gacgttgtat cccgagaggt
      181 cggtctcagg actgatagcc catgatagtt cgatatgcgc cggaacattg ccctgcgacg
      241 gcacgggcac tacgatcaat ccctgtggag ccgcgggagg aaaggtatcg cgcggcgtaa
      301 ggatcacgag attcgaatcg gtggattcga gcgttccgtt cggatattgc gcgaagctgc
      361 gcaccgagta tgcgtacgtg gcgccgaaat tgaattggct gtcgcgaaac gactgggaat
      421 ccggaggcgt ttctccgatt tgggcgagcg gagatttcag cagcggattc tgggttgtgg
      481 cggtggaggg cgcttctgca cgatagatgc ggtaacccgc aattatcggc gtggaatcaa
      541 cgagtgcctt aggagccgtc caagtaatta cgatggccga gggagtcacg tcggttttca
      601 gatcgtcgat cgcttgcggg gcaggctgaa cgctaataac cgccgcgttt gaattcgccg
      661 atgatttttt cggtgaagca agtgtgcgaa cgatgtaaac ggcaacgttc cccgcgtgct
      721 gcgcgaaatc ttcagcactg agcgcatcgg cgtaacgcac gcggccctga tcggaatagc
      781 ggcccgccat ggacgctgga accgtaattc gcagcgtcgg attatctggc gcttgcaaag
      841 tcgcgttgcc gctcgcggga gtcggctcaa aatcgcggta gatttcgaac gccaacggag
      901 cgctgagggg ccggtggtcc atcgcttcat tcggcagcgt gaatgaaagc actacgtcat
      961 tgcccgacct ggtggccgtg aggtcgctca cagcttgcgg aatgggcggc cttcgctcat
     1021 atggttcgct cggcgaggcg cagccggcga tgagaaaaga aaatagaagg accgagaaga
     1081 gaatgcagaa agtcgcaaat gggcctgacg ccgcggcgca ggaagctttc gcagcgcgcg
     1141 cccattttct gttttccatt aggcgcttta cagaatgtag cggcttagat cctggtcctt
     1201 gacgatatcc gcgagctgct tttgaatgta tggagcatcc agcttaacgg ttttcttttt
     1261 catttccggc gcctcgaaag aaatttcttc gagcactttt tcgaggatgg tgtgcagacg
     1321 ccgcgcgccg atattctccg tctgctcgtt gacgcgcgcc gcaaaccgcg cgatcgaggc
     1381 cactgcgtct tccgtgaacg agagtttaag tccctcggta tccagcaggg caatgtattg
     1441 cttgatcagg gcattctttg gctcggtgag tattcgcacg aagtcagcct cggaaagcga
     1501 gcccaactcc acgcgaatcg gaaagcgtcc ttgcaactcc ggtatcagat cggagggctt
     1561 cgcggtgtga aacgcaccgg ccgcaatgaa gaggatgtga tccgtgcgca ccattccgta
     1621 ccgcgtgttc acggtggtcc cttcgacgat gggaagaatg tcgcgctgca ctccctcgcg
     1681 agaaacgtcc ggcccgtgcc cggactcgcg ccccgcaatt ttgtccactt catcgatgaa
     1741 caggatgccc atttgctcga cgcggtcgac ggcgatccgc gtcacctggt ccatgtcgat
     1801 cagccgattt tcttcctctt gaatcaggta atcgaaagcc tccgagacgg tcatcttgcg
     1861 cttcttcttt tgctggccga acatgccgga gagcatgtcc tttacgttga tatccatttc
     1921 ttcgacgccc tgattggtga tgatttcaaa tgagggcatc gaacgctcgc gaacttccac
     1981 ctccacgagc ctttggtcga gctttccttc gcggagctgc gcgcgcattt tgtcgcgtgt
     2041 gcgctgggcc tgctcgcggc gctgcgcctc cgccgctggt cctgcgtccg atggcatggg
     2101 cgacgacggc agcaataggt cgagcaggcg ctcttccgcg gattgctccg cgcgctcggc
     2161 cacttcatca agcttctctt cgcgaaccat atcgatcgac gtttccacca aatcgcggac
     2221 catggactca acgtcgcgcc ccacgtagcc cacttcggtg tatttcgagg cttctacctt
     2281 tacgaagggg cagccggcga ggcgcgccag acgccgggca atttcggttt tgcccactcc
     2341 ggttgggccg atcatcagga tatttttggg gagaatctct tccgcgagtt cgggctgcag
     2401 cttttggcgg cgcacacgat tgcgcagcgc aatagcgacc gcctttttcg ccgcattctg
     2461 ccccacaata tatttgtcga gttcgaccac gatttctcgc ggcgttaatt cgtcaaaaga
     2521 aggaagcgct tcggcctccg cgggctgctg ttctccggtc aagtagatca ccatgtcttt
     2581 ctccggcttg ccgccgctca taactcttcg atgctgaaat tggcgttcgt gaagatgcag
     2641 atatcgcttg cgatgcgcat cgcttcctgc gcgatgtctt tggcggtcat cttggtatgc
     2701 tttgccagtg cgcgggcggc ggcgagcgca tagggaccgc cggacccaat cgcgcagata
     2761 ccgtcatccg gttcgatcac atcgccattg ccggaaagca ggaaggtgcc cttcgcgtcc
     2821 gcgacgatta agagcgcatc cagatggcgc aaggaacggt ccgtgcgcca ttccttcgcc
     2881 aattcaactg ccgctcgcgc gaggtttcca tggtattcct gcagcttgtt ttcgaagcgc
     2941 gcaaaaagcg acaaagcatc cgccgtgctg ccggcgaatc ccgcgaggat tttatcgctg
     3001 tagagccgcc gcgtcttttt cgcgctatgt ttgatcacgc cctcgcccag cgtgacttgt
     3061 ccatccgcag cgagcacaac cttggtatcc ttgcgcacac acaacaccgt ggtgctgcgt
     3121 atctgcgccg atttcttgct tatctgcatc cggcgattat accttcgccg cgcgcaatcg
     3181 gcgaatcgca gtgaaaaatg aattgatgaa ctccgcgcta gggatcggcc ctctcaggct
     3241 cgtgcagatc tattctgagg aagaagatga ggatgaactt ctgcccgctt tttcgtcctg
     3301 cttctcgtct ttcttcttct gctttgcttc cttctctctc tgtttctttc ctaccttagc
     3361 ccaacgctca tccaaggact ctttggtgaa gagtgagtcg gataggccag tgttgtagtc
     3421 gcatttgtcg aaaaatgctt ggaataactt tataccgttc cgctcgcgcg ttagttgaag
     3481 tgcggtctga atgccgtcct gcggatggta attcgcaaag taatcgatct cttcgctttt
     3541 cgtctgcgtt tttggatccc gggtctcagc cacttcccga ataggcaggt gggtcttgtc
     3601 cgaaaacgcg atgcgaatcg tttgattgtc gctatcgacc aattccaccc attttgcctg
     3661 cctcagatcc acggtgtccg agccgccata ccgcaaggtc atgcccgcct catgaattcg
     3721 atggcgcagg atgttgtcga tgtccctctt agcgccttcc ttaaaatcct ccatatccgc
     3781 gcgaggagcg ttcgaaacgc cgccgcggtc gagctcccat ccttggtcgc cgttgtagac
     3841 ggagatgata ttgcgcttgg gtaaattctc ctggcgctcc ttgagcggtg gcttctggta
     3901 gtcgatgaag tgcccgaagc ccgtaagttc gcctgagtga tcgaactggc cgagcctgcc
     3961 ggtgcaggtc acgtcccgga cgttcagata cgcctcgccg ccaagcgctt gtatggcttc
     4021 cgcgaggatc tgcttggctt tcgccgcgct ttgttcaggc attagaaact ggtcatcctg
     4081 agcggacgcc tttggcggag ctgtggtcca cgccatcatc agcatcaccg ccgtcgcaat
     4141 cgctgctttc cttatccgca ccgaaacaaa cctccattga caatcaatcg ctttgcgtcc
     4201 gcgcaaaacc cgccggtttc tctgtatcct gcgatcatgc gcaagatccg ccttaaacga
     4261 ttcgatgcgc gtacccggct tttgggtctc ctgctttgct tctcgtcccg ctcggaattg
     4321 caccttgagg ttattacacg atggcggggg ccttccgtat agccgcttct ttcaccaagc
     4381 tgccaaacaa ggacagcgtc agcggatccg attccatcat acgctccggg tgccactgca
     4441 ctcctaccac ccagttttca tcgcccgtcc attcgacggc ttcgattacc ccgtctggag
     4501 cccgcgccgt gacccgcaag tcgcgtcccg gcatcaggac cgcctgatga tgggagctgt
     4561 tgacgatagt ttcccgtgcg cctgccatct cgctgatccg cgattcgctt tcgaccgtga
     4621 tgacgtgatg tgcatcaagc gccccatcgt cggaatccga atgcacaatt tttgttccaa
     4681 tctcgctcgg cacatcttga atcaacgtgc cggacaaaaa aacgttcaga ctctggattc
     4741 cgtagcaaat cgccagcacc ggcttccgtg cagcgaagca gttttccagg agcgcgaaat
     4801 cgattctttc ccggttatcg tctgcttcag cacatttggc gtgcctcggc gcgccatatt
     4861 gagaaggctc gacgtccgcg gggctgcccg ttaagacaat gccatcgaga gaagatacca
     4921 atgcggcgaa ggcatcatga gatgcggcta gcgaaaccgg cacaggttgt ccacccgccc
     4981 ttcgcaccgc cgccaagtat ttctcaatct tattggtccc gcgagaaact tcttcttctc
     5041 gcgtccggta gggaacgcca atacgaggcg cgtggttttt gtgatccatc agacgcaaaa
     5101 tcgtaccatc accaccgcgg ctcggcaatt ttagcggcgt ccggcccaat aggcgcgcag
     5161 atcgccgaca aggtaaagcg agcccgtcgc gaaaatcgcg tcgttaggag acgccatctc
     5221 aatcgcatgc tctagagctt tggcgggatc cgcaatgacg gtcgccttcc tcgcaagatg
     5281 cgccgtcatt tcacccagca cgggcgctga aatcgcccgg ggctgcatgg gctcggtaag
     5341 caccacgaaa tccgccgccg ggaataataa accggcgatt tcgtccacag ctttgtcgcg
     5401 catggcgcca taaaccagca cgacgcgacg gcccgcgaaa ttctcctccc aaaatttcag
     5461 aagttcctta gctcctgcag gattgtgcgt gccatccaga aagatggcag gccgttcgct
     5521 cagacgctcg aggcgccccg gccatcgagc cgcccggatg ccgcgttcga tcgcttcctg
     5581 cgtgatcgaa aagccgcggg tcgccagcaa acgcgcggct gtcaccgccg caagagcatt
     5641 gcgtatctga aaccgcccgg ccagcggcgg agcaattgaa aaacgctcct gcgatcctgt
     5701 cgtcataacg tttgcgcggt aaaggccacc ggagtcctcg acggagtcga tacgccacgc
     5761 ctcgtcaatc tccactaacc gcacccgcat ttcctcgcag cgttgcgcga tcacggcacg
     5821 cgcttccggc ctctcgaccg aactaaccac cgtcgcgcca gccttaatga tgccggcctt
     5881 ctctcccgca atttctgtga tcgaatggcc cagaaaactc tcatggtcga aatcaatcga
     5941 agtgatgatc gctacctccg gccgaacaat gttggtcgaa tccagccggc cgcccaagcc
     6001 aacttcatac actgcgaatt ccacgccgct tttggcaaac gccacgaacg ctagagccgt
     6061 gatgcactcg aaataggtgg gatgcgcgga gagttttcca ctggccaaaa ggccttcgat
     6121 cgccgcgcga acgcgcgtcc acgcggcggc aaatgcctca tcggaaatat ccgcgccatc
     6181 aattcgaatc cgctcgttga ttcgttccag atggggagag gtatacagcc cgctgcgaat
     6241 gccggccgct cgaaggatcg aatcgagcat cgccgccgtg gaacctttgc cattcgttcc
     6301 ggcaatgtga acgcagggaa tacgaagatg cggattgccc agcgcctcgg aaagcgccgc
     6361 tatattctcc aggccgaact tgtgcacgtg ggcttgctgc ggcgatgcca gctcgcggcc
     6421 tagagccagt agcgcgcgca cggactcgtc atacgtcatg gggaatcgag gaagaaccgc
     6481 agcgcctgcg caatgtacga cttcaattcc ttgcggtgca caatggcatc gagaaaaccg
     6541 tgctccaata gaaactccgc cgtctgaaaa ccttcgggca atttttggcg gatcgtttgc
     6601 tcgatcactc gcggcccggc gaagccgatc atcgcgcccg gctcggcaat attcaagtcg
     6661 cccagcatcg catagctggc gctaacgcct ccggtggtcg gatcagtgag caccgaaata
     6721 aaaggcacgc gcgaatcatc gagccgagcc agtgcggacg ccactttcgc aagctgcatc
     6781 aggctcaccg cgccctccat catccgcgcg cccgccgagc aggacacgat gatgagcggc
     6841 tgcctttctg ccatggaccg ttcgatggca cgcgccactt tttcgcccac aaccgcgccc
     6901 atcgatccac caatgaaccc aaactccatc gagcagcaga ccaccggcct gccgcagagc
     6961 attccctgca ctgtcagaat tgcatccttc atcccgagct tttcttgggc ctcggctaaa
     7021 cgctgcgagt acggtttcgt gtcggtgaaa tgcagcggat cgctggatgc gagttcggcg
     7081 tcatgctcgg tccattgcgc gtcatcgagt aacaattcca gacgacgccg tgccgaaagg
     7141 cgaaaatggt gatcgcactt cgggcaaact tcccagtttg cttccaaatc ctttttccag
     7201 atgatggcgc ggcagccgct gcacttgatc caaaggccct cggtgcggac gcgccgctcg
     7261 tccaccggcg ttggattctc aatcgcgctc ttttcgcgct taaaccaggt catggttaga
     7321 cgtgcagccc cgctgcttgc aacctaataa tcaatcccac gttgcgccag gacgcctttc
     7381 gtgtagggat gcttcacttc cctcatttcc gtcaccagat cggccgcctc gaccaacttc
     7441 ggatgcgcgt ttcgccccgt gcagatcacg tggacgcgct caggacgctg cgtaagcgat
     7501 tccaccaccc gttcggggtc cagcatctta tagctgatca cgtagttgat ctcatccagc
     7561 accaccagat cgtatttctc gccgtaaatc tgttctcgcc cgtagttcca ggcctcttca
     7621 cacaggcgca gatcttcagg atcggtttcc gcaccgccca ccttcacgaa cccgcgcccc
     7681 atcggacgaa tctcaaaccg gtcgtcgccc aacattttgg cggcgtcgag ttcgccgtaa
     7741 tgccaggaac ccttgatgaa ctgcaccatc aacactcgca gtccctgccc aaccgctcgg
     7801 aaagaagtcc ccagggcgca ggtcgtttta cctttgcccg gcccggtgta aacaatcagg
     7861 agtcctttac cttccggcat cgaatcgcat ctcgtcgaaa accgtccaca atagtcgccg
     7921 gagcctcggt aaaggtcaag caaaccggta acggcggcat tgagtcgatt gtctgtactc
     7981 gctcgccagt gaattagtac aatccgcgcc gtagctgaaa ccagcgcgcc aagcagagag
     8041 gacgaatatg cgcaatcgat ggatgaagcg tgcgctctgg attgtattgc cggtcatgac
     8101 aattggggcg attctgctca acgttggcgc cccggctggt gccgccgaaa ctccacctct
     8161 tgcagcggac gcctgcgact atggttgcct aacagcaatc gtcaaccagt acctgaaagc
     8221 tcttgtcgcg cacgatcccc ggcagattcc ggttgcgagc ggcgtcaaat tcactgagaa
     8281 cacgattccg ctgaaacttg gagacgccct gtggggcaca ataagcgggc tcggaactta
     8341 caagctttac ttcgccgatc cacaggacgg cacggcaggg atcgaggcgt cgattcgcga
     8401 aaacggaacg ccggcaatcc tgctgctcag gttgaaggtc gtgaaacgca agatcagcga
     8461 gatcgagatg ctagttcacc ggaatgcgca ggacgcgaca gcgctcgaga aattcggcca
     8521 tccgaatgcc gtctggtcgc agccacttaa gccctcgcaa cgcgtctcgc ggcaagaaat
     8581 ggtgaaaatc gcgaattcat atttcgacgg cattcttcac tctgcgggcg attcggtgcc
     8641 ctttgattcc agatgcaacc gtattctcga cggctcccag gacacgaata accccacggc
     8701 caaaggctgg tttgacaaag attcgttcaa gcctgatgcg atgggaattc gcgagaacat
     8761 gaacacgcac atctggtcct acattcattc cattgatccg cggcgcattg tggtggtcga
     8821 tcagaaaatg ggaatcgttg taggaatgtt catgttcaat catcctggaa acgtgaagtt
     8881 cgcggacgtg cccggcgtcg gcaaagttcc catgccgccg gtgacgcaga gaccatcgag
     8941 cgtcttaatg ggcgaatttt tcaagctgga aggcggaaag atacggcaga tcgaaggaat
     9001 ttccgtcgcg ctgccctacg gagccaagac cggctgggaa actgccgcca gtaaaaatta
     9061 acgactgagc ctgcaagctc ggtcgcgagg cttgcaggcc cggttccatt ttgttttgcc
     9121 gctccttcac cgtgttgtct tcgcttatcc aagagagaag acaacttgca cagtcgtatc
     9181 gacctccact ggctgcccgt tcagcattgt cggacggtag cgccattctt tcaccgcgct
     9241 catcgccgac gccatcagca gtgccggccc ggatacaaat tgcagtttct cgaccgatcc
     9301 atccttggca atgatcgcgt gcaagaccac cgtgccggaa acgtgagctg tctttgctat
     9361 cgccggatat tgaggctcaa ccttgttcac cagcgatgcg gcttgtacgt tgccacccac
     9421 gtgtatgcgc ttcggcgcag ctggtggagg cggtgcgggc gcggcgcccg tgccaactcc
     9481 tccgagcaca tctcccgttc cgccggcaac tcccggcata tcgttcgggt tgactgccgg
     9541 cggcgcttcg tctttcgcga tctcgatctt tttcggaatt accgtcggcg ccgccatttt
     9601 gttaacggcg ataactttca ccggcttgac ctgtttcaca accgcctgct gcggcggtgg
     9661 cggtggcggc ggtggtgggg gcgccaccag aaacgtggtc agcatcgtct tgggcagcac
     9721 ttgcgtgtag atcagcggta tcagcagcag cgcgcccaag attcccgcct gaagaatcac
     9781 cgagaggcct atggaccatg gcttgtgagt tcgggccatc tctcccgtct gccccgaaac
     9841 aatgagttcg ttaaacatag gcacctcctc gctctgctcc ccatttgatg tttcggctat
     9901 gggtcgagaa gcgcaagggg cgttccaaac tcatccgccc taactcctta aaatacagcc
     9961 gcagatttac ctgtattcgt actaggtcca ggcaataact tcactgcgct atcttcggct
    10021 ctcggctgta catgcttgat acagcgcggc ttacataaaa tcctgtatct tagcccccat
    10081 atgcgtgaga tcgctttaca ggcgcctatc ggggtgggga tttagagcgg aatactgaca
    10141 aggaaaagta agagtggatg gcctttgcag tttttgagag aggtttaggc gcgcgcggta
    10201 ttgatccagt cgacggtttt gttgaagccg ccgaagacga agaagtgtac gcccacaacg
    10261 ccgaggggca gcgcggaacc gttcgggggc ccgagcattt cggccacccc gcgaatgagc
    10321 ggctcgggac ctttgtcgat cagcagcttg gcgaattgcg gtttttcggt gagcactttc
    10381 atggaagcgc ccacgccgca gaccagtgcg tagcgtgtga gcgaaatcag gctggccgga
    10441 ccggcaaatc ccaagcggat cggattatcg attccctgcg cgcgaacctt gcgaagccac
    10501 tcgaccacag gttccgacgc aaacgcgaat tgggtgacga tggtaaggtt cagcgagttt
    10561 tcccgcgcga atttcgtctt ggccgcaata gcttcccaga gcagcggctc ggaaattttc
    10621 ggattgcctt ccggaaatcc gccgacggag atacgcgtga ttccggcctt ctgaagaatg
    10681 ccgctctgca ttaccgcaag gcttgagtcg aatgggccag caggctcgtc gcggtcgccg
    10741 gcgatgatca ggacgcggtc gactcccacg gcaaccagct gcctgacaaa ctcctcgagt
    10801 tgcgctgcgc tttccacgtg gcgcgcgccg atatgaggcg tcgggaagag ccctgcgctc
    10861 cgcagtttgc cggccgggcc cacggtgtcc aatggattct tgccaggaat ccaggtcatg
    10921 aatacttccg tgccgggttc gaggcggttg ggagcggcgt cgatgatttt cgattcgccg
    10981 ggattcactt cgatggaaaa gccgcggagc atgtccgcga cgctgggtgc ggctgcaatt
    11041 tctgtcatag ggataacatt atatgatgcg cgcgtgttgc gagccaagcg gtggcctccg
    11101 gcgaaggaaa acatacccca cagcggcctg aaaagcggcc ggcttccctt cctgcgccac
    11161 gtccgaccag tgccatcagg gttttccgcc gtgtgttagc cataggagtg gtatggcggc
    11221 aatcgccgct gcgacgatga gagcgaggaa gatcgttccc agtatcgtga cgaaccggct
    11281 attggcgtgc gtccccatga tctttttgtc cgcgcccgct cggagaatcg ggtagtaggt
    11341 gaagggcatg acgaccatgc cgaaaataat ggcgatgttc acgagggtga gcggccgcat
    11401 gccggtgaag tcgatgataa gcgcaaaaac gagcattccc agccagccaa ccgtgaagac
    11461 gggaacctgc tttgcccgcc ggttcttgcc ccatttgaaa ttgaagaact ggcaaacgtt
    11521 gtaggcacct gagagtcctg tttcgatggc ggctccgccg atgcaagcca tggctcctat
    11581 cacggcgacg gtgagcgctt tgccggcgaa gggaaaggct cccgccataa tggaactgct
    11641 aagcatctga gggaagatcc ggtgtggcag gaagacgagc gcgcccagca ccatgaggcc
    11701 gaccgtcagg accgagccaa ggatcgatcc gagcgaggcc accatgaaat tctcgccgag
    11761 gtcctgcggt ttccaatctt cctcgatggc tcccgtcgaa taaaaatgca cttcgtactc
    11821 catcagcatc gcgctgaaaa tcccgacagc gaagtaggca tacagaagaa cctgcttgcc
    11881 atcggccaca tgcggtgcgg ggtttaggcc ggcaagaagg tggctccagt ttgggtgcag
    11941 ggctgccgcg gatactccga aaacgatcat caggagccct gataagccga agctgcgctc
    12001 gatccactga aagctcgaca gatgaatgat ggctccgagg gcgatgcaac tgccaatcaa
    12061 tacggcacgt tccggccacc cggtaagaag gtgcagcagg atcgccaagc cgccgatttc
    12121 ggcggcacaa gttatgaggt tgagcagatt ggaggcaatc agcgttacga gtccaaggtt
    12181 gtaacccagc cgtgcgcgga ccacggagaa gacgggttcc cgcgcgacaa ccgcgacccg
    12241 gccacacatc tccatataaa taatgattcc gacggtaccc agcacaatcg accagagaag
    12301 cttgtagcca aacagggcgc ccgcctgagt ggtgaacacg atctggccga gatcgacgaa
    12361 gccgccgagt gccgtcatga tcccgagggt gatgccgagc agctttctca tagtcccgct
    12421 ttcgcggctg tgagagattt caggatggcc tgcacgcgct gccggatcgc tggaaagtct
    12481 ttgccgtggc cttgtttctc gctaatcagc gccaactcgc gcgagagcag atcgagctgc
    12541 tcgatgcaaa gacgaacgac ctgtaactgg ctgggctgag ggctggactt gcgcaggcct
    12601 ttggcttcgc gcttggcttc gtcgcgtaag taggcggcat gtccggactg aaatgccggc
    12661 aggaggcggc ctccctcaat ctggccggcg aaaatatcgg tttccgacgc tagagaaatc
    12721 gacgacgcca gattagaacg cagctcggcc gaactgaggg tcttggcgcg gccgcacgac
    12781 tcgagcgcga cgacagtcag aacaaggaac gcttgtttgc gtaccatagt gcagatgccc
    12841 aaaggtctga tgaattggag tctcgttgtg tctcgttgta tggagccggg gcaaagaagt
    12901 atgatgcaga attcgcaagg agacccacgc cgcagggcac aacgctgcgc tttccgagag
    12961 agaaagacgc cgaattcggg atccgtggag gaacaaatga gcagggtgct gaattatagc
    13021 ctcgtcgccg tcgcagcgct gatgatggcg agctcggcgt tcgctcatca ctcgcacgcg
    13081 atgttcgata cggataagaa agtgacgctg gcgggcaccg tgaaggaatt ccagtggagc
    13141 aatccgcact gctggattca agtgcttgtg cccgatccga aggacccgaa agccccgccg
    13201 gtagaatggg gcgttgaaat gggggcgcca attcaattaa ttcgtcacgg ctggaaagcc
    13261 gggagcttga ggcctggaga caagatcact gttgtgatta acccattgcg cgatggccgg
    13321 cccgggggac aaatcgtgtc cgcaatcgga ccagacggca agccgatcgg cgcggtgccg
    13381 gaaactccgg cgccataagc ttgaggcgga agctaaacat ggtatgaaag ctgacttcag
    13441 cggctgaatg caaaagggcc cgcctgaagg cggccctgcg aaaacgaaac cgccccccga
    13501 gcggagttaa atggcttcac gcgcgccaag catctgcgcc gggcatagca tgctatgccc
    13561 ctacgagggc aaaggcgagg gtttgtgatg attcgaagaa ttactatcgc ggcaatgcta
    13621 gtggcgggct gcttctacat ctcgcaggca cagtcatctt ccaatttttc caattccaag
    13681 gcgcgtgtgg aggctttcgc caagctgcca gattggtctg gattgtggga atttgatatc
    13741 tggactgacg aactggacgg gcagcaaatg ggcccgatag gaatgcagag agcaaaaatc
    13801 tatgccgcgc agatgcaggc gacgtttact cccgcatggc aagcgaagct cgcgcaacta
    13861 aagaagacgg cagatgcggc caaagccaaa gacggcagcg aaccgcccgc gttcgaggaa
    13921 ttcggacaat ttggagggtg cggctggacg cccggatttc cggccacaat gctgcctggg
    13981 acgtatgagt ggcgagtgac tcctgaagaa gcgacactca tcagccttct gggttcggtg
    14041 cgacacatct acacggacgg gcggccccat ccgtcgaagg acgagctgtg gcccatgagc
    14101 cagggcgatt ccatcggaca ctgggagggg gatacgttgg tggtcgacac tgtgtcgata
    14161 aggtcgccgg tgtattttcc gggattggcg agaggcgtcg gagttccgtt cattccgatg
    14221 agcaatgaat tgcataccac cgagcggatt cg
//
LOCUS       DATNDT010000260         2705 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2599228, whole genome shotgun sequence.
ACCESSION   DATNDT010000260 DATNDT010000000
VERSION     DATNDT010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2599228"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            59..349
                     /locus_tag="VN788_08385"
     CDS             59..349
                     /locus_tag="VN788_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HXU20584.1"
                     /translation="MADNAGSKISFFLVGMGIGALIGVLFAPKSGEETREYLSSRADE
                     GKEYAQKKARELRERGEDLLERSKEIMSRQKDAISSAVDAGKETYKREAKIS"
     gene            365..868
                     /locus_tag="VN788_08390"
     CDS             365..868
                     /locus_tag="VN788_08390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20585.1"
                     /translation="MPGWVEAFIVIAAIAIILQTAILAGLFFETRTAVRNLTRIVSQL
                     QSRIDPILIRTNRILEDSESRIASVMEDASELTRLARNQAQKVDRVVTDMAERLRVQV
                     IRADRILTGTLEVIEETGTTVRSKIWQPVHQVSAVLKGVKAGLDFLRQRRPPEGEATT
                     EDEELFI"
     gene            complement(939..2141)
                     /gene="mnmA"
                     /locus_tag="VN788_08395"
     CDS             complement(939..2141)
                     /gene="mnmA"
                     /locus_tag="VN788_08395"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HXU20586.1"
                     /translation="MTSTSQFEITSGSPVGISASLSPPAEMVAVAMSGGVDSSTVAAI
                     LRERGQAIVGLTMQLWNQRRLPQLQGEGPVQHRCCSLDDVHDARRVAEHLDIPFYVIN
                     FEREFENTVVRPFVEEYLAGRTPVPCTRCNNDVKFEHLHATARQIGAARIATGHYARV
                     RRNTETGRYELLRAVDESKDQSYFLFGLTQEQLARAEFPLGELTKQEVRDIARRLAVP
                     VAEKPESQEICFVPSGSYVQFIENYIQEQGNVSRDASDETTKLGGPGEIVSTSGEVMG
                     HHNGLHNYTVGQRRGLGFAVGHPVFVVALDRASNRLVVGEDGELRRSVCELRDVNWIP
                     FAAPDAPVRARVRIRNRHEPAEAEIMPLGEGRARVSFAEPQRAITPGQAAVFYRGEIV
                     LGGGWIAE"
     gene            complement(2149..>2705)
                     /locus_tag="VN788_08400"
     CDS             complement(2149..>2705)
                     /locus_tag="VN788_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXU20587.1"
                     /translation="YVRSGTPLAPRFYGGHAERDRRPGTENVPGIVGFGKAAELARKL
                     LAEDSVRIRQLRDRLESALLERIPGAQVNGDRARRVPNTTNMTFTNAGGEALLIALDL
                     QGIACSTGAACSSGSTEPSHVLLAAGLTHDDARSSLRFSLGRATTREEVERAIEVVPE
                     VVERIRSLSPRAPVAAQWAAVPIR"
BASE COUNT          554 a          867 c          731 g          553 t
ORIGIN      
        1 ggcactatcc ttgcctgtca cttcggggct atgctactgt cgaaatagga ggtgcaacat
       61 ggcggacaat gctgggtcga agatcagttt tttcctggtg ggcatgggaa tcggggcgct
      121 tatcggagtt ctttttgcgc caaagtctgg cgaagagacc cgcgaatatc tttcctcaag
      181 agccgacgag ggtaaggaat atgctcagaa gaaggcgcgc gagcttcgcg agcgcggcga
      241 agacttactc gaacgcagca aggaaataat gtcccgccag aaggacgcca tctcgtctgc
      301 cgtggacgcc ggcaaggaaa cttacaagcg ggaagcgaag atttcgtagc gcggaggctt
      361 ggtcatgcct ggttgggttg aagctttcat cgttatcgcc gctatcgcga tcattctgca
      421 aaccgctatc ctggccgggt tgttcttcga aacccgcacc gccgttcgca acctcactcg
      481 tatcgtttcg cagcttcagt cgcgcatcga tcccatcctg atccggacga accgcatcct
      541 tgaagattcc gaatctcgca ttgccagcgt gatggaagat gccagcgagc ttacccgttt
      601 agcacgaaat caggctcaga aagtcgatcg cgttgtcacc gacatggccg agcggctccg
      661 cgtacaggtg atccgcgccg accgcatcct caccggaact ctcgaggtaa tcgaagagac
      721 gggaaccacc gttcgttcta agatttggca gcccgtacat caggtgtcgg cagttctcaa
      781 aggcgtgaag gcgggactcg atttcctgcg ccagcggcgt ccgcccgagg gcgaagccac
      841 cacagaggac gaagaactct tcatctaagt tcgtctaatt ggcacgcacc gttccgctcg
      901 ttggcccttc gcggccgcac ccttggtccc gggccaagtt attccgcgat ccagcctccg
      961 cccagcacga tctctccgcg ataaaaaacc gccgcctgcc cgggtgtaat cgcccgctgc
     1021 ggctccgcaa acgatacgcg cgctctcccc tcaccgagcg gcataatttc ggcctccgcg
     1081 ggttcatgcc gattacggat tctcaccctg gcgcgcaccg gcgcatcggg tgcggcgaac
     1141 ggaatccaat tcacgtcacg caactcgcaa actgaacggc gcagctcgcc atcttctcct
     1201 acgacgagcc gattgctggc ccggtcgagc gccacaacga acacaggatg ccccactgcg
     1261 aagcccaggc cacgccgctg tcccactgtg taattatgta aaccgttgtg atgtcccatc
     1321 acttcacccg atgtagagac gatctctcca ggtccgccga gcttcgttgt ttcatcgctc
     1381 gcgtcacgcg acacgtttcc ttgctcctga atatagttct cgatgaattg cacgtagctt
     1441 cccgaaggca cgaaacaaat ttcctggctc tccggttttt cggcaacagg cacggccagg
     1501 cggcgcgcga tgtcgcgcac ctcctgcttc gtcagctccc ctaggggaaa ttcggcgcgc
     1561 gccaactgct cttgcgtcaa cccaaaaaga aaataagatt gatctttcga ttcatcaacc
     1621 gcgcgcagta gttcgtaacg ccctgtctct gtattgcggc gcacccgcgc gtagtggccg
     1681 gtcgcaatgc gcgccgcacc aatttgccgc gctgtcgcgt gaagatgctc gaacttcaca
     1741 tcgttgttgc accgcgtgca gggaaccggc gtgcgccccg cgagatactc ttccacgaat
     1801 ggccgcacca cagtattctc aaattctctt tcaaaattaa ttacgtagaa tggaatgtcg
     1861 aggtgttcgg ccacgcgccg cgcgtcatgc acatcgtcga gcgaacagca acggtgctgc
     1921 accgggcctt cgccttggag ctgcggtaat cggcgctggt tccaaagctg catcgtcagg
     1981 ccgacgatcg cctgtccgcg ctcgcgcaaa attgccgcaa cggttgagct gtccacgccg
     2041 ccagacatcg ccaccgccac catttccgct ggaggtgaaa ggctcgcgga aatccccacg
     2101 ggactaccgc tcgtaatttc aaattgtgag gttgaagtca tgctcgaact aacggatcgg
     2161 aaccgcggcc cactgtgccg caacgggcgc tcttggcgaa aggctgcgaa tgcgttccac
     2221 cacttctgga accacctcga ttgctcgctc tacttcctcc cgtgttgttg cgcggccgag
     2281 gctgaatcgc aggctcgatc gcgcatcatc gtgtgtaagt ccagccgcca gcaacacgtg
     2341 cgaaggctcc gtcgaacccg aagagcaagc cgcacccgtc gaacacgcaa tcccctgcag
     2401 gtccagcgcg atcaaaagcg cttcgccgcc cgcgtttgta aaagtcatgt tcgttgtgtt
     2461 tggaactctc cgcgcccgat cgccatttac ctgcgcgcca ggaattcgct ccagcagagc
     2521 cgactccaac cggtcgcgaa gctggcgaat gcgaacgctg tcttctgcca aaagtttccg
     2581 cgccagctcc gccgctttgc caaagcctac aatccccggc acgttctctg tcccaggacg
     2641 tcggtcgcgt tcggcatgcc cgccataaaa tcgcggcgcc agcggcgtgc cggatcggac
     2701 ataca
//
LOCUS       DATNDT010000261         7050 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_868046, whole genome shotgun sequence.
ACCESSION   DATNDT010000261 DATNDT010000000
VERSION     DATNDT010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7050
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868046"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="VN788_08405"
     CDS             <1..726
                     /locus_tag="VN788_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4070 domain-containing protein"
                     /protein_id="HXU20588.1"
                     /translation="MGGFIVGFDSDPPDIFDRQIEFVEHAAIPWAMTGVLQAPPTTPL
                     YDRMRTEGRLLKSNPEPSNFDPPNFRTVLPLPELLDGTRRMLLTLYDPRQFYKRVLDS
                     LERWQLQPEQRASGVSLLYLLSALLKSFWKQGVLSNYRRAYWGYMRRLILRWGLHPQK
                     RRLGFELALSGHHFIRYARKVAESLEIQSRLAIQPIPHGFVPHSDEPTRNNLVQINSV
                     PGHSGFARSASGQATQQSQNSDL"
     gene            complement(2393..3217)
                     /locus_tag="VN788_08410"
     CDS             complement(2393..3217)
                     /locus_tag="VN788_08410"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXU20589.1"
                     /translation="MTLTLLTKRLFRSIARVVPRCGMVSQAVAFNMFLSFFAILLAVL
                     GMLRLYLEGTRGQELVPRLQAILPPGSWQLVSNFVFRPDVSAWYSTVLGWVGTLLVGL
                     QTMKLMMEGFHMISGSRSTQAFLGRQLRALLLFAVAACTWLVAVALSVFGRPLRQLMI
                     NKFGDSLLIRASWSGIFSLAAMILAMLVLAFIYRFAEPRAATWSSVLPGAAIATGLWW
                     AANELFGIYVRKTQYGPVYGGLAAVIGLLGWMDLSVMLVFLGAAWNRERDERSFPQ"
     gene            3926..4327
                     /locus_tag="VN788_08415"
     CDS             3926..4327
                     /locus_tag="VN788_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20590.1"
                     /translation="MKGFVVVVALLAIWPMSAGKKYRMTADASVPAASGEIGVQTDKS
                     NGNMKVDIKVDNLAQPANLTPPENAYVVWIRPSGEEAHKQGALGVDKNLKGELKVITT
                     SKNFDVFITAEQSGSVTVPSGIQILQAHVSQ"
     gene            complement(4920..6656)
                     /locus_tag="VN788_08420"
     CDS             complement(4920..6656)
                     /locus_tag="VN788_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXU20591.1"
                     /translation="MPIRYDAIVIGTGAGGGTLALHLAQAGKKILILERGPFMPQEKL
                     NWDTSAVFLDNRYHTKEIWQDKHGKELHPQQSYFVGGQTKVFGAAMFRMRAEDFGVIR
                     HQGGLSPAWPISYADMEPFYTRAEELFQVHGDLGSAPSVPGGYGSSFDPTEPFHSKNY
                     PFPALPNEPCMQSIEDAVRELGIHTFPIPLGLKRNETAPLESKCVRCDTCDGYPCLVH
                     AKSDADINCIRQILHLPNVTLMTNSRVTRLLTNANGTEVAEVEVIHSGSGVGSEDFAP
                     PASGGRTVSYSADLFCLCAGAVNSAVILLASANDKHPNGLANSSDQVGRNFMYHQADA
                     LLAISTERNEDSYTKTWGTNDFYLRDTDPNYPYPLGQVQPVGSFHSEMMKSEAPPLTP
                     GFVLEAMKHHAVPWWLTTEDLPDPDNRVTLDNATPLAVQNSQPGLGGPHPSGDTGRTN
                     ESEQVSDNAPYRIQLSYTPNNVKSFERLKDRWVDILKRAGHAATSVPLHAYFKKRIPI
                     EGVGHQNGTCRMGRNPSSSVLDPNCKAHDLDNLYVVDASCFVSASAVNPSLTIIANAI
                     RIADHLVSERLK"
     gene            6863..>7050
                     /locus_tag="VN788_08425"
     CDS             6863..>7050
                     /locus_tag="VN788_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU20592.1"
                     /translation="MRLQDKVAVVTGAGTGIGQAIALAFAEAGAAVVVDYVGSPSVSE
                     DTIKKISAIGGKPLGVDA"
BASE COUNT         1644 a         1879 c         1958 g         1569 t
ORIGIN      
        1 atgggcggtt tcatcgtcgg ctttgatagc gatccaccgg acatatttga ccgacaaatt
       61 gagtttgtag agcatgccgc catcccctgg gcaatgacgg gtgttttgca ggcaccgccc
      121 acgactccgc tgtacgaccg catgcgaaca gagggccggc tcctcaagag caatcccgaa
      181 cccagtaact ttgatcctcc taacttccgg acagtgctgc cgctgcctga actgctcgac
      241 ggcacgaggc ggatgctgct tacgctttac gacccgcgcc aattctacaa acgtgtgctc
      301 gattcactcg aacgctggca gcttcaacct gaacagagag cgtccggggt ttccctgctg
      361 tatttattga gcgcgctgct caaatctttt tggaaacaag gcgtgctttc gaactatcgc
      421 cgtgcgtact ggggctatat gcgaaggctc attctgcgct ggggattaca ccctcagaaa
      481 aggcggcttg ggttcgagct cgcgctctcc ggtcaccact tcatccgtta tgcacggaaa
      541 gttgccgagt ctctagaaat tcaaagccgc ctggcaattc agcccatacc gcacgggttt
      601 gtcccccatt cggatgagcc aacacggaat aatctggtgc agatcaacag cgtgccagga
      661 cattctgggt tcgctcgttc agccagcggc caagcaactc aacagagcca gaattctgat
      721 ctctgatcag ccgctagttc caaacggttt cagcaaattc cgcaactacg gcctggctcc
      781 aagtggcagc gtcactccgg tcttcttcgg tgggacaatc gatctggacc aagccgtgaa
      841 tcgaacggtc agcagggcgt gtgaattggc cctggtttcg cataataaaa ttaagacaat
      901 cggccaatat ctcagactga taagagttcg caaaatggac tctgctaact tccgaggtgg
      961 aagaagcaga atcaggcggt gcaacagaat agcggcagac caaggtccca tagccgcgat
     1021 tcctaatcgt ccaaaccgac agcgagctca atggacaatc tcgttgtttc tcatattcga
     1081 catgcaccga gcaagggtcg cccggtttaa ccacgtcttt ccaggatgcc acgacagcaa
     1141 gctcaagcgc acgatcaagt tccatgtcag ccagctttca accgtactgt ttgacccgga
     1201 tgctccacaa tgttgacgcg gcgcaaaatc gtctgacgcg aatccccacc ggttacaatt
     1261 tgccttgcca tcgaacttca ccctcctggg gcaaacatcg tcgacgctga cagtaagaac
     1321 acggccagag cgatcggtaa tacgcgcatg gttttcattt tgaagttctc ctcctgtcac
     1381 aaaccggcag ccgttgctgc acctgactgc catttcggtc cttcccgaac gagctcgcgg
     1441 gtcctcgcga gttcccatgc cgtagggatt ggatagaagt caattgcgtc tggctcggct
     1501 ggctctcctc gtcagtcaga agaaccatgc ctctctcaat gtgccgcgat tgagcatcga
     1561 tgctcatata aggcattccc acaaaccgtt tcgccgctat tcgagtaatc tgcagagaat
     1621 tgaagtttcc cgacctctgg ttcctcagaa tgcggctaac tgcccggcgc accgctttct
     1681 gcctattcat accaaaagcc agtgcgccga tttccccagc caagcaaaaa aatgtccatc
     1741 cggttttttg aatctctcga tccagtctgt atccgtcgaa tttcttcact aggctccaac
     1801 gtgggacaca gggttcgctc tcgatcggca aggcttctgg caagagagtg ccttccttga
     1861 tcaagatatt gcctgcatga atttcgcatc ccacttttct cctccatgca cgtcagtttg
     1921 ggatctgatt tttgttggat cccgcctcct gacaaggctt atgttagacg aagccatgcc
     1981 tgacaactgt ctcattgcgc acacttgggc tcgttgcccc aatcgatctg agcgaacgct
     2041 gtccgacctt ccgtcgaaca ccaccaggcc tttctatgca aagcttcacg ttttggtgcg
     2101 tgttctgctt gaaatggctc gactccgact cattcgcttt aagcgatagt aaagagtcta
     2161 ttggcggcgg catctggcac tcgctccccg cggaaagatc gtcgtctccg gcattgtggc
     2221 cttctcgccc atcgatgccg acagcgtctg aaagcgtgcc tgctgccatc atatgaatcg
     2281 tttctgagca gatgttggtg tggcggtctc gagagaaaca gatcttaaag aacggtcagt
     2341 gtttatcgca aagcaattgc gccattttgc gaagggtcat ggttcggccg tgctattgcg
     2401 ggaaggagcg ctcatcacgt tctcggttcc aagccgcgcc caaaaaaacc aacatcactg
     2461 aaagatccat ccaacccagc aagccgatta ccgctgccaa cccgccatag acaggcccgt
     2521 actgcgtctt ccgaacgtaa attccgaaca gttcattagc ggcccaccat agaccggtcg
     2581 ctatggccgc tcccggcagg acggaactcc aggtggccgc gcgaggttcc gcaaagcggt
     2641 aaatgaaagc caaaacgagc attgccagga tcattgccgc cagagagaag atgccgctcc
     2701 acgaggcgcg gatcaaaaga gagtcgccga atttgttgat cattaattgc cgcagcggtc
     2761 ggccaaaaac gctcagagca acagccacga gccatgtgca ggctgcaacg gcaaacaaaa
     2821 gaagggcacg gagctgccgt ccgagaaacg cctgcgtcga gcggcttccg ctaatcatgt
     2881 gaaacccctc catcatgagc ttcatcgttt gcaaacccac cagtagcgtt ccaacccagc
     2941 cgagaacggt ggaataccaa gcgcttacgt ccgggcggaa aacaaaatta gacacgagtt
     3001 gccaactgcc tggaggcaga atcgcctgaa gccgaggcac aagttcttgt ccccgcgttc
     3061 cttccaggta gagtcttagc atccctagaa ccgcgagcaa gatcgcaaag aaggaaagaa
     3121 acatgttgaa ggcaactgcc tgtgaaacca tgccgcatcg aggaacgact cttgcgatgg
     3181 agcggaagag tcttttcgta agaagggtca gcgtcattac cgaaatccta ctacggcgta
     3241 cgaaagtgtg aacatgaaga gaatttcgtt acatcaggga gtcgtcgcac tgaaagcatt
     3301 atcgcggata tccacacgtt cttcacggcg gcaaattgga cggtaatgtg aaggtattca
     3361 gtcccagaaa aaggtgtaac gccgactcgg tcgcctgagg tagcagcgtc gcatacgcgt
     3421 cgtccgaacg atcattccgt gaccacggct gcctcggtgg tctgaagatc gcaacccggt
     3481 agctgacggg tgctgaagca tcgccgtgag cgcgtgccct acatgtgaac aaggcgaacc
     3541 tatactgcgg cgcccttcct cagaagcgcc ttacccacca tcaattctta gggccaagtt
     3601 ctgcgcccgt tctgccccaa tcttgctgtt cactatgaga tagaccatgc gcggtgccag
     3661 ctccccgata acatccagtc gccactgcgg ggggcaagcc gtccactgga ccaaaatggt
     3721 gccgacatct cggaaatgta gcctcccaaa atgacatgta tagaaagaga cagactcgat
     3781 ttagacataa ttgatataaa cagttatgga gagagaatct gagtatcaac ggcaagtagc
     3841 ggcacgggtc ctctcgggca cacgtttcat tcccacgctg ttcagtcgat tccggattca
     3901 catttgacgt ttcttggagg tccgtatgaa aggatttgta gtggttgttg ctttgcttgc
     3961 catctggccc atgtctgctg gaaagaaata ccggatgacc gccgacgcca gcgttccggc
     4021 agcaagcggc gagatcggag tgcaaacgga caagagcaat ggaaacatga aggtggatat
     4081 aaaagtggac aatctggccc agcccgcgaa tctgacgcca ccagagaatg catatgttgt
     4141 ttggattcgt cctagtggcg aagaggccca caaacaggga gcgctcggtg tcgacaagaa
     4201 tctgaagggc gaattgaaag taatcactac ttcgaagaac ttcgacgttt tcattactgc
     4261 tgagcaaagt ggcagcgtga ctgtgccttc gggtatccag attttacagg cgcatgtaag
     4321 ccagtaaagt ggggaagccg ggtcgcgtaa tgtcgcgccg aagccccgcg aagatggaga
     4381 tgatgtgatt cttgaaaaga gcgcgcgggt tgaaactcac tgtcatggcg cacccgctag
     4441 cgatggaggc aacatgcaat cggggcaacc atatgatatc aagatcgaca agctgatcac
     4501 cgagagagcg taccagtgtt gggaatggcg gggccgcccc ttcggatcgc ctgaagttga
     4561 ctggtatcga gctgtggatg atttcaaaca cgaacagcaa tggcgtggcc tagcatgatg
     4621 gcttgctgag agtgtatccg actggagcct cgtgcatccg ctaacgactc cattggcgat
     4681 gcggcaagaa atagaattcg gatggttgcg tggtccgcgt aactcgtttg cgaagaagaa
     4741 gtttagttgt ggggcctgca accgtccgaa ttggttggta ttatcgtttt ctcttgaatt
     4801 gatacggaaa gccgcctaag aagcatgacg acaatccagg gttttatcgg ttcgcctcca
     4861 cttaactgca tttttgacga tttgcttcct cagggtcaaa cctagagtac taccggcaat
     4921 catttgagac gttccgacac gagatggtcg gcaatccgga ttgcattggc gatgatagtc
     4981 aatgagggat taaccgcgct tgccgagacg aaacaagacg cgtcgacaac gtataggttg
     5041 tcgaggtcat gggccttgca gttcggatcg agaacgctcg acgacggatt tctccccatt
     5101 cggcaagttc cgttctggtg gccgacacct tcgatgggaa tgcgtttttt gaagtaggca
     5161 tggagaggca cggacgtggc tgcgtggccc gctcttttga gaatgtcgac ccaccgatct
     5221 ttgaggcgct caaaactttt cacattattg ggcgtgtacg agagttgaat tcgatagggg
     5281 gcgttgtcgg aaacctgttc ggattcgttg gtgcggcccg tgtctcctga gggatgcggg
     5341 cctccaagcc ctggctgact gttttggacg gcgagtggcg tcgcgttgtc taaggtgaca
     5401 cggttatcag ggtcgggcag atcctccgtt gtgagccacc aagggacggc gtggtgcttc
     5461 atagcttcga ggacgaagcc gggcgtcagg ggcggagcct cacttttcat catttcggag
     5521 tggaagctac ctacgggttg gacttgaccc agtggatacg gataattcgg gtcggtgtcc
     5581 cttagataga agtcattcgt gccccaagtc ttggtgtaag agtcctcatt gcgttccgtc
     5641 gagatggcga gcaaggcatc cgcctggtga tacatgaaat ttcggccgac ttggtcggag
     5701 gagttggcga gcccgttggg atgcttgtcg ttggcggatg ccaggagaat cacggctgaa
     5761 ttgacagcac cggcacaaag gcagaaaaga tcggcagagt agctcacggt cctcccgccg
     5821 ctcgcgggag gagcaaagtc ttcagagccg actccggagc cggagtgaat cacttcgact
     5881 tccgcgactt ctgtgccgtt cgcattggtg aggagacgcg taactcgcga gttcgtcatc
     5941 aaagtcacgt tgggcagatg cagaatctgg cggatgcaat tgatgtcggc atcggatttg
     6001 gcgtgcacca gacacggata accgtcgcag gtatcgcagc gcacgcattt ggattcgagc
     6061 ggtgccgttt cattgcgctt cagcccgagc gggatgggaa atgtatggat tcccagttcg
     6121 cgcacggcgt cttcaataga ttgcatgcat ggctcattgg ggagcgctgg aaaaggatag
     6181 ttcttcgagt gaaacggctc ggtgggatcg aaggagcttc cgtagccgcc gggcacggag
     6241 ggagccgagc cgaggtcgcc atgaacctga aagagctctt cggcgcgggt ataaaaaggc
     6301 tccatgtcgg cgtaagaaat cggccatgcg ggagataggc cgccctggtg ccggatgacg
     6361 ccaaaatctt ccgcgcgcat gcggaacatg gctgcgccga acactttggt ctgtccgccc
     6421 acgaagtaag attgttgcgg gtgcaattcc ttgccgtgtt tgtcctgcca gatttctttg
     6481 gtgtgataac ggttgtcgag aaaaacggcg gaagtgtccc agttgagctt ttcctgaggc
     6541 atgaagggcc cacgctccag gataagaatc ttcttgccag cctgcgcgag gtgcaacgcg
     6601 agcgtcccgc cccctgctcc ggtgccgatg acgatggcgt cgtatcgtat aggcatgctg
     6661 actacgatag cagaccggct cgatccaagg ctgttgtcat tcgcggatgt caagactcga
     6721 gattgccgca attggcgcta atgcttgtgg gcgatggtcc gacagatgat tcctacggcg
     6781 ccagcatgaa aatcgtgttc ccataggaac aaccgcgttc gtcgtttagt ttaatcgaga
     6841 accggaacta agaggtcatc cgatgagatt gcaagataaa gtagcggtcg ttaccggtgc
     6901 cgggaccggg atcggtcaag ccatagcact cgcatttgcc gaagccgggg ccgccgtcgt
     6961 cgtggattac gtcggcagcc catccgtttc agaggacacg atcaagaaga ttagcgccat
     7021 cggcggaaag cctctcgggg tcgatgcgga
//
LOCUS       DATNDT010000262         4423 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2059427, whole genome shotgun sequence.
ACCESSION   DATNDT010000262 DATNDT010000000
VERSION     DATNDT010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4423
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2059427"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="VN788_08430"
     CDS             <1..257
                     /locus_tag="VN788_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007805483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXU20593.1"
                     /translation="ILGIAKPLGAVALLVPGVPKLKEWAYAGFTFAWISAFVAHYLAK
                     DGPTAFMPLILLVFLFISYATRPASRQIERQIASSPIVGE"
     gene            complement(288..464)
                     /locus_tag="VN788_08435"
     CDS             complement(288..464)
                     /locus_tag="VN788_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20594.1"
                     /translation="MPEFGDYQRRMWAGEVDLADKDVKIVNGRVHWERLLQNVRQARY
                     NEALRIVSDRHATP"
     gene            843..1244
                     /locus_tag="VN788_08440"
     CDS             843..1244
                     /locus_tag="VN788_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HXU20595.1"
                     /translation="MQETATQLEPQQIPDKLYFRIGEVSRLAGIKTYVLRFWETEFPS
                     LGPKKSGTGHRLYRRKDVELVLEIKHLLYERRYTIEGARKFLESRGKMPDTAAAETKK
                     GRRKQRELFEEPVPGLAQIRRELTEILDLLR"
     gene            1325..1558
                     /locus_tag="VN788_08445"
     CDS             1325..1558
                     /locus_tag="VN788_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003866711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar FlbD family protein"
                     /protein_id="HXU20596.1"
                     /translation="MHLTRINRTPFVLNSELIGHVDATPDTVVSLTTGEKIMVLESAD
                     EIISRIVDFRAAISVRSAQLQHDLRELMEVKST"
     gene            1574..2422
                     /locus_tag="VN788_08450"
     CDS             1574..2422
                     /locus_tag="VN788_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein"
                     /protein_id="HXU20597.1"
                     /translation="MSANETRVKSGGRVDFASVGGILIAIAGIVGGLIMEGGKVRDIA
                     QITAALIVLGGTFGAVMLNTPAPVLFRSLRRLRGVFAGTSPGTRETLSVIFNYAAKAR
                     RTGLVSLEKDALSEPDPFLRKSLNLAIDGADLKDIRAMMELEIDIEEQRAEQEAHVFE
                     SAGGYSPTIGIIGAVLGLIQVMKNLANIDQVGHGIAVAFVATIYGVALANVFLLPVAN
                     KIKTRARQETEAKQMMLEGVAGIVEGLNPKLIRSKLEAYLEPAQAARESSGPVAASKR
                     AAAAGA"
     gene            2432..3319
                     /locus_tag="VN788_08455"
     CDS             2432..3319
                     /locus_tag="VN788_08455"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HXU20598.1"
                     /translation="MRSSTRRHSASTHASHERWLVSYADFITLLFAFFVVMFATSHAD
                     RAKAQQVSDSVKKALNNSDLRAVALAILGSAARTNGPAKAQTPLAAIKPATAQIIPQI
                     AELEPSLKLLTDQLSDEIREGKVQIHMEARGLVISFRQAALFPSGQDEIARDTWPSIG
                     KIAAAMKEIPNPVRLEGHTDSVPIHNARFRSNWELSAARSIALLDLFTDHFGVPAEKL
                     SISGYADNDPTATNDTEEGRARNRRVDIVFLNATGMLAEPGQPAAPVPPLSAIAPKSP
                     VDKPAPVPPAVPPGRSALP"
     gene            complement(3799..>4423)
                     /gene="rnc"
                     /locus_tag="VN788_08460"
     CDS             complement(3799..>4423)
                     /gene="rnc"
                     /locus_tag="VN788_08460"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXU20599.1"
                     /translation="SLASEKNGGGGDNEQLEFLGDSVLGFLASEILVRRFPGSPEGRL
                     SKLKASLVSATHLHEVAQKLELGDHLLLGRGEEMSGGRAKKALLANAVEALIAALYLD
                     GGMQAARRFVEASVVGVPNSGDGGDEATDDFKSALQEMAQALKLPQPRYSTVEERGPE
                     HAKTFTVEVRVGRDLVSRAEGVSKKSAGQNAAKQILERIGDAQGRVG"
BASE COUNT          951 a         1362 c         1183 g          927 t
ORIGIN      
        1 tcattctcgg gatcgccaag cccctcggtg ctgtggccct cctcgttccc ggcgttccga
       61 agctgaagga gtgggcatat gccggattca cattcgcatg gatctcggca tttgttgcgc
      121 attatctggc gaaagatggc cccaccgcat tcatgccgct catactgctg gtgtttctat
      181 tcatctccta cgcgacccga cccgcaagcc gccaaatcga gagacaaatt gcgagttcgc
      241 cgattgtggg cgagtgacgc gccactcgga agttgcgccc acggacgtta tggggttgca
      301 tgccgatctg aaacaattct gagtgcttcg ttgtatcgtg cttgccgcac gttttgtaag
      361 agccgttccc aatgaaccct gccgttgacg atcttcacgt ctttgtccgc caagtcgact
      421 tctcccgccc acatgcgccg ctgatagtcc ccgaattcgg gcacggttcc actccaatcg
      481 atcggcttgc ccgacgaacc caaaaagaca cgcgccgcag gcaccccctc cggcgagcaa
      541 tgcttcacaa ataggaatgg tgcttcgtta tgtcgatgct tttattgcta tttttcaatt
      601 cgatcgccgc gcatataacc cgcgcacgag aggactggta agcgcggata tttcggaaaa
      661 cgcttatcgt ttttcgagag ttcgcaaaga tagaacaagg cttggcgatc ggactgaatg
      721 cgccggcaat ttacacacga tgcgcaaagc ccggccttcc gctcttcggt catctatcca
      781 gcttcctgcc ttccatgccg tgagtaaatg ccagttcaga caagctagaa tacacctaag
      841 ggatgcaaga aaccgcaacc caactcgaac cgcagcaaat acccgacaag ctgtacttcc
      901 gtatcggaga ggtcagccgg ctcgcgggca tcaagacgta cgtgctgcgt ttctgggaaa
      961 cggaattccc ttcgctgggc cccaaaaaat ccggcacggg tcatcggctg tatcgccgca
     1021 aagacgttga gttggtgctg gagatcaagc accttcttta cgagcggcgc tacacgattg
     1081 aaggcgcacg caagtttctc gaatcgcgcg gcaagatgcc agatacggcg gccgccgaga
     1141 cgaagaaggg ccgccgcaag caacgcgaac tatttgagga acccgtccct ggactcgcgc
     1201 agatccgccg ggaattgacc gagattctgg atctgttgcg ttgacatgtt gagttgaccc
     1261 ggatcgaatc taatcttttc cgtaccaccg ccgataagac caagtaggga ggttacggct
     1321 cacgatccac cttactcgca tcaaccgcac tccattcgtg ctgaactctg aactgatcgg
     1381 ccatgtcgat gctaccccgg acaccgttgt gagccttacc acgggagaaa agattatggt
     1441 gctcgaatct gccgatgaga tcatttcacg catagtcgat tttcgcgcgg cgatctcggt
     1501 ccgttcggcg cagctccaac atgatttgcg tgaattgatg gaagttaagt caacttaggc
     1561 aggcgattca ttcatgtcgg caaacgaaac aagagttaag tctggtggtc gtgtcgattt
     1621 cgcgagtgtc ggcggtatcc ttatcgctat cgcgggaatc gtaggcggcc ttattatgga
     1681 aggcggaaaa gttcgcgata tcgcgcaaat taccgccgcg cttatcgttc tcggaggtac
     1741 attcggcgcg gtgatgttga acacccccgc gcccgtgctg ttccgttcgc ttagacgtct
     1801 gcgaggtgtt ttcgcgggca ctagtcctgg gacgcgcgaa acactctccg tcatcttcaa
     1861 ttacgcggca aaggcccggc gcaccggtct tgtctcgctc gagaaggacg ctctttccga
     1921 accggatccc tttcttcgta aatcgctgaa tctcgccatc gatggggcgg atttaaaaga
     1981 catccgggcc atgatggaac tcgaaatcga catcgaagaa cagcgcgccg aacaggaggc
     2041 gcatgttttt gagagcgccg gcgggtactc tccaaccatt gggattatcg gcgcggtcct
     2101 cggcctcatt caggtgatga agaacctcgc caacatcgac caggttggcc acgggatcgc
     2161 cgtcgccttc gttgccacga tttatggggt tgcgctcgcg aacgtctttc ttcttccggt
     2221 cgcgaacaaa atcaaaacgc gcgcgcgaca ggaaacagag gctaaacaga tgatgctgga
     2281 gggagtggcc ggaatcgtgg aaggcttgaa tcccaaactg atccgcagca agctggaagc
     2341 gtatttggaa cctgctcagg ctgcccgcga gtcttctgga cccgtcgccg cgtccaagcg
     2401 cgcggccgcg gctggagcct aacgaatcat catgcgttcc agcacgcggc gccactccgc
     2461 ctccacgcat gcaagccacg aacgctggct tgtctcctac gccgacttca ttacacttct
     2521 tttcgcgttc ttcgtcgtca tgtttgcaac ttctcatgcc gaccgcgcga aagcccaaca
     2581 ggtatcggat tccgtaaaga aagcgctcaa taacagcgat ctccgggccg tcgctctcgc
     2641 gatcctcggg agtgccgcgc gaacaaatgg tccagccaag gcgcaaaccc cgctggcggc
     2701 gatcaaaccg gcaaccgcgc aaataattcc gcaaatcgcg gagctggagc catcgctaaa
     2761 gctcctgacc gatcaactct cggatgaaat tcgcgaaggc aaagtacaaa tccatatgga
     2821 ggcgcgcgga ctggtcatca gctttcgcca agcagctctg ttcccttccg gccaggatga
     2881 aatcgcgcga gatacatggc ccagtattgg caagattgcc gccgccatga aggagattcc
     2941 aaatccggtc cggctcgaag gccataccga ttcagtgccg attcataacg cgcggttccg
     3001 tagcaactgg gagctctcgg cagcgcgaag catcgctcta ctggatctct ttacggatca
     3061 ttttggggtg ccggcggaga aactttcgat cagtggatat gccgataacg accctaccgc
     3121 gactaacgat acagaggagg gacgcgcccg caaccgccgc gtggatattg tattcttgaa
     3181 cgcgaccgga atgctggcgg aacctggaca accggctgcg ccagttcccc cgctgtccgc
     3241 catagcgccg aaaagccccg ttgacaagcc tgctccagtg ccacctgccg tgccgccagg
     3301 ccgatcagct ctgccttagc aattagtcat gctcaagccg gacacaacac acaatgaaaa
     3361 tagacacgag gtgagatgtg ctatcgccgc cgacgtgcgg aatagaattt gtgcatcctt
     3421 ggcgctagtt gagctcgtcc agcagtttaa tcctggacgc cttcggtaca atacactgtt
     3481 gccccagcat gcggggagca cgcgagagaa tcaatgcttc cgggcggaag atgcgagata
     3541 tcgtgcatcg ccgatgtagc ctggacgtcc acaaaaacag attgcggtgc cagggaattc
     3601 atagtcctga ttccaacgtt cctgtggcgg ccaatcctgg ttctaaaccc tgaaagcggg
     3661 ctgcagactg aaacgcctgc gccgccgcga aggcgatccc gatacagggg cgtcactgaa
     3721 aatactctgg cacgcgcgcc gtccacgctt cctgaacacc cgcgcggctc cgcggcgccg
     3781 attcttgcgt cgcgcggact aacctactcg cccctgcgca tcgccgatac gctccagaat
     3841 ttgcttcgcc gcattctggc cggcgctctt ctttgatacg ccctccgccc ggctcacaag
     3901 atcccggcca acgcgcacct caaccgtaaa tgtcttggcg tgttccggcc cgcgctcctc
     3961 cacagtcgaa tatcgcggct gcggcagttt aagcgcctgc gccatctcct gcaacgcgct
     4021 tttgaaatcg tccgtggcct cgtcgccacc gtccccggag ttagggacgc ctaccacgct
     4081 cgcttccacg aagcgccgcg ccgcctgcat tcctccgtcc agataaagcg ccgcgatcag
     4141 agcctcgacc gcatttgcca gcagagcttt tttggcgcgg ccaccactca tctcctcgcc
     4201 tcgccccagc agcaaatgat cgcccagttc cagcttttgc gcgacttcat gcagatgcgt
     4261 cgcgctgacc agcgatgcct tcagcttcga caaccggccc tcgggcgagc ccggaaaacg
     4321 ccgcaccagg atttcgctcg ccaggaagcc gagcaccgaa tcccccagga attcgagctg
     4381 ttcgttatcc ccgccgcccc catttttttc cgaggcgagc gac
//
LOCUS       DATNDT010000263        11746 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3794644, whole genome shotgun sequence.
ACCESSION   DATNDT010000263 DATNDT010000000
VERSION     DATNDT010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11746
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3794644"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1240
                     /gene="hisD"
                     /locus_tag="VN788_08465"
     CDS             <1..1240
                     /gene="hisD"
                     /locus_tag="VN788_08465"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HXU20600.1"
                     /translation="LTSATAKKILASRSAISRDAARIATRIVNDVRRRGDKALFAWTK
                     RLDHLGLNSESVWVSRAELSSARKEISREFFAAMDHAARNIRAVARRQKPREWTMQVE
                     RGVRAGQRVRAIDTIGCYLPGGRFSLVSTLLMTVIPAQEAGVREIVVASPQPGASLLA
                     AAERLGVKLVAKIGGAQAIAALAYGTRSIPRVDKIFGPGNRFVTAAKQIVSSDCAIDM
                     LAGPTEALVFATHGNPRLIASDLIAQAEHDPDAISLFVTTSSALARAVASEIDRQLAE
                     LPKMNLAPRSLGKSGAALVAPNLTAAVDFVNRFAPEHLSLPDGTKGLLAKIGSAGSIF
                     LGGWSAQSFGDYASGTNHVLPTGGAARSRGGLSVSDFVKCISVQEVSRAGVARLAPVV
                     AEFARAENLEAHARSVEMRK"
     gene            1237..2292
                     /gene="hisC"
                     /locus_tag="VN788_08470"
     CDS             1237..2292
                     /gene="hisC"
                     /locus_tag="VN788_08470"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HXU20601.1"
                     /translation="MTRAMPVRRAVEQMRPYHPPLEGRIDKLRLDFNENPIGCSPAVR
                     RALAKLSAASISAYPEQETVRRKAARHFGVRPEELLLTNGTDEALSLVVNTFVEPKDD
                     ILLVEPTYAMYRFYSELAGARIVAPRYDEAMCFPWNKVLQALRAGPRVFFLPNPNSPT
                     GNLLTLKELQRILKAAMRTMLVIDEAYFEFSGVTVIPWIGRHKNLIVTRTFSKAAGMA
                     GLRLGCIFVHRELASIMRKAQSPYPVNAAALAAAEAAMRDRAFIARTVREVRRARKEL
                     EHGLARLEVRCFRSGGNFLLVFFGPRAKEIVRALTRKGTLVRDRSSDFGGEGYVRITL
                     GTLQQTRRLLRELESIL"
     gene            2289..2876
                     /gene="hisB"
                     /locus_tag="VN788_08475"
     CDS             2289..2876
                     /gene="hisB"
                     /locus_tag="VN788_08475"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HXU20602.1"
                     /translation="MRKANLRRKTKETDIGLRLNLDGRGKSRISTGIRFFDHMLEQIA
                     RHGALDLQLVARGDLDVDQHHTVEDVGIALGEGVKKALGSKRGILRAGYFLMPMDDCL
                     AAAALDFSGRAFCMCSFKISAARVGDFQTELMEDFFRAFAQAASANVHLKLVYGRSSH
                     HQVEAIFKAFAKALRFAVTRDKQLRRVLPSTKGVL"
     gene            2873..3478
                     /gene="hisH"
                     /locus_tag="VN788_08480"
     CDS             2873..3478
                     /gene="hisH"
                     /locus_tag="VN788_08480"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HXU20603.1"
                     /translation="MKVAIVDYGAGNLPSVERALRGLGAETERATEPSQIAAAKAIVL
                     PGVGHFSAFVNGLRERNLTPSLREAYDFGVPILGICLGLQAMFASSEEAPGAPGLGFF
                     PEEVRALPTNVKSPHIGWNQLRRMEDSALLHGIPEDAYFYFAHSYAAPASAGPTVAAC
                     DHGFPFAVVIEQKRLAAVQFHPEKSGETGARVLRNFLDSAQ"
     gene            3475..4239
                     /gene="hisF"
                     /locus_tag="VN788_08485"
     CDS             3475..4239
                     /gene="hisF"
                     /locus_tag="VN788_08485"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HXU20604.1"
                     /translation="MTLARRIIPCLDVDGGRVVKGIEFEGLRDMGDPAELGARYNREG
                     ADELVLLDISASVQGRATFLETIRRVAEKLNIPLTAGGGIRKMEDGRAVLRAGADKVT
                     VNTAAVERPELLSELAQQFGAQAVVLSMDVKRKSDSWEPYTRGGRDATGLDAAVWAAE
                     AVSRGAGEILLTSIDRDGTCSGFDCELTAKIAATVSVPVIASGGANTFDDFLAVFNQG
                     SADAALAASIFHSETHSIRALKQYLGARGVPVRLSR"
     gene            4252..4980
                     /locus_tag="VN788_08490"
     CDS             4252..4980
                     /locus_tag="VN788_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HXU20605.1"
                     /translation="MIIPCIDLQGGKAVQLIRGRRRALAVSDVLGLLERFRDYPILHV
                     IDLDAAMQKGSNSRWIKLLCRRARMKVRVGGGIRTVARAEKILSWGAEKVIIGSAAFR
                     NGRVDRDFLKAMVSSVGCKRVIIAIDTEGGQIVVRGWRERLKLKPSDVIPELEPFCSE
                     FLSTFVDNEGTMKGTDLRWFRKLRSVTSMPITAAGGIRSMREVRALEKTGMHAAVGMA
                     IYTGKLAGDKRALGGPMLSPTSLK"
     gene            5153..6598
                     /locus_tag="VN788_08495"
     CDS             5153..6598
                     /locus_tag="VN788_08495"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HXU20606.1"
                     /translation="MKTQLRVMKFGGTSVGDASCINRVAQVVKEAARECPIVVVVSAM
                     SGVTNWLIQAANRSEAGDREHSHAIFAELRKQHAAALETLVRDKEKRRRVVSCLEKLF
                     NEGEELCKGTALLRELTPRTLDSISSLGERLSAPMVAAALSEHDVPTEAVESTELIVT
                     DSYHGGADPLMDRTHARSNARLRPLLERGIVPVVTGFIGATDSGVLTTLGRGGSDYSA
                     TILGAALSADEVIIWTDVNGVLTADPRLVPEARTIPEISYLEASELAYFGAKVLHPKT
                     LRAVAQAGIPVWIRNTFAPEQAGTKITPRGRSNGGGVKALTAIRDVALITVGGPGIVG
                     VPDVVGRTFSTTAEVHASILLISQSSSQNDICLIVAASDAKRTVEALRKEFAQDVAHE
                     KVEHITMDPSIAIVAVVGENMRGTPGVAGRTFNAVGRENVNIIAIAQGSSESNISFVV
                     SEKDMKVALQATHREFNLSASVPFESEVKVS"
     gene            6600..8012
                     /gene="thrC"
                     /locus_tag="VN788_08500"
     CDS             6600..8012
                     /gene="thrC"
                     /locus_tag="VN788_08500"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HXU20607.1"
                     /translation="MTEKTARAPEIITEKAVQRCIDPACGATYDLNERIYLCSRCGGT
                     LEISQNLSEIGDATALRKRWSARAASRDPRDASGVWRYRELLPFDDAAPFVTLFEGNT
                     PLYHGPRSARYCGLDDLRLKHQGCNPTGSFKDTGMTTAVTQAVVLGARTVVCASTGNT
                     SASLAAYAGRAGLRAGVLLPRGQISSGKLAQSLDYGALVCEIDGNFDDCMRLIQELGE
                     DPSIYVANSINPFRIEGQKTVAFEMMEQCAWQVPDHVVVPGGNMGNSTAIGKGFQELL
                     DLGLIHRLPKLSAIQAEGAAPVANLFAKLKADQLTGEMPLPEVMETVENPRTLATAIK
                     IGAPVSWKKALRAILRSGGKVIAVSEQEIADAKAMIGRDGIGCEPGSATTVAGIKKLV
                     AAKHIRSHDSVLAVLTGNMLKDPSYVSHYHSGTLVLDSPGGPPQRIVGSFQNAPKTVA
                     ATKAAILAALAQAGKEGNLP"
     gene            8053..9006
                     /gene="thrB"
                     /locus_tag="VN788_08505"
     CDS             8053..9006
                     /gene="thrB"
                     /locus_tag="VN788_08505"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HXU20608.1"
                     /translation="MTSARQPSFEVRVPASTANMGAGLDCLGLALEMYLSVRATVLSK
                     PGSRTLARSRGVRGTSDLPRDPEENLIFRAMRHTVEREGFVLPRVRLAVQNEIPVAGG
                     LGSSAAATVAGVALGFAVAGRQVPKDVALRYAAEMEGHPDNVAAALLGGLVVTFTRSD
                     GTVAAVRRHWPKVIRLIVVTPNMKLETKKSRAALPATVSRADAVHNLQRSALFVAALE
                     DRRYDLLWDAMQDRLHQTPRQSLIPGLAEALALPRMPGLLGVALSGAGPSVMALATGR
                     HDEIGKAIAQCFERHDLAPTIRNMEAAQEGLVSTQKYISWK"
     gene            complement(9050..9898)
                     /locus_tag="VN788_08510"
     CDS             complement(9050..9898)
                     /locus_tag="VN788_08510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20609.1"
                     /translation="MIRRGSVVGWVYLVTLMCLFSVTFWGTVVTERNSAKQERAQTAN
                     AAKSFVTPARPDTSDSPLDAVAGSRRLFPYSVIPGGVESAEELRKALEHDPIAAAHYA
                     GFDVSKTHVIRLDSDEEMYVSYRLNNRIYWTKKKLKLLRGQTLITDGKNTARTRCGNR
                     LSATARSPVAEKQPLDMILDPAPLPDLAEARPIALPMALPFPAFSSSLVPSSDFGGGA
                     DNSPVFPISGGGSSSHNTIIPPTTPPTIPPAATPEPDSFVLTAAGISIVLASGWFGYF
                     RRRVQN"
     gene            complement(10235..11467)
                     /gene="tyrS"
                     /locus_tag="VN788_08515"
     CDS             complement(10235..11467)
                     /gene="tyrS"
                     /locus_tag="VN788_08515"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013908509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HXU20610.1"
                     /translation="MTPTQFKPVEEQVAYLRKGAAEIIREEELKAKLDQSAKSGKPLR
                     VYLGVDPTAPDIHLGHTVVLRKLKHFQDMGHTAIFLIGDFSAMIGDPTGQSETRPPLT
                     REQVDANAKTYLEQVYKILDREKTEVRFNSEWLAKMTSYDTVRLCGHYRLARMLERED
                     FRSRLEKNQPISVHELLYPLLTAYDAVALKSDVELGATEQKFNLLVHREIQREYGQTS
                     QAVLTMPILVGLDGERKMSKSLGNYVGITEAPAEMFGKLMSISDDLMWSYYELLTDFP
                     PPVIVRLREEVRMGVLHPMDAKMQLAHTVIAGFHGEEAARRASDKFRRVFRERQAPAE
                     APRHTIPQGAPKRLSVLLVELELASSRSEAERLMKQGGVELEGARVDDVKREIDLSKP
                     GEYLLRAGKKKFLRLVIE"
     gene            complement(11464..>11746)
                     /locus_tag="VN788_08520"
     CDS             complement(11464..>11746)
                     /locus_tag="VN788_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HXU20611.1"
                     /translation="GLEPQRQAIDHGVKFSGCTVHFVDENLDAGPIILQAVVPVEDDD
                     TPATLAERILREEHRVYTEAVRMVLEGRCRIVGRRVLVDGGGNGPGEHR"
BASE COUNT         2351 a         3450 c         3489 g         2456 t
ORIGIN      
        1 gcttactagc gcgaccgcaa agaaaatcct ggcttcgcga agcgcgatca gcagggacgc
       61 cgcgcgcatt gccacacgta ttgtgaatga tgtgcgtcgc cgaggcgata aggcactatt
      121 tgcatggaca aagcggttgg atcacctggg gctgaattca gaaagcgtct gggtgtcgcg
      181 cgcggaattg tccagcgccc gcaaggaaat ttcgcgcgaa tttttcgccg ccatggacca
      241 cgctgcacgg aatattcgcg cagtagcgcg tcgccagaag ccgcgtgaat ggacgatgca
      301 agtggaacgc ggcgtgcgcg ccggacagcg cgtccgcgca attgatacca tcggctgcta
      361 tcttccggga gggcgatttt cgctcgtttc cacactgttg atgacggtga ttcctgcgca
      421 agaggcgggc gtgcgcgaaa tcgtagtggc aagcccgcag cccggcgcgt cactactggc
      481 agcggcggaa cgacttggcg tgaagcttgt cgccaaaatt ggcggagcgc aggccattgc
      541 cgcgcttgcc tatggaacgc gctcgattcc gcgcgtcgac aaaatcttcg gtcccgggaa
      601 tcgtttcgtc accgctgcga agcaaatcgt cagcagcgac tgcgcgatcg acatgctggc
      661 aggaccgacg gaggcgctcg tatttgccac ccatggcaat ccgcgattga tcgcatcgga
      721 tcttatcgcg caggccgagc acgatcccga tgcgatttcg ctgttcgtca ccacgtcttc
      781 agctttggct cgcgccgtgg ctagcgagat cgaccggcag cttgcagaat tgcctaagat
      841 gaaccttgcg ccgagatcac tgggcaaaag tggcgccgcg ctggtcgcac caaacctcac
      901 agccgctgta gatttcgtaa accggttcgc gcccgaacac ttgagcttgc cggatgggac
      961 aaaaggattg ctagcgaaga tcggttcggc tggcagcatt ttccttggcg gctggagcgc
     1021 gcagtccttc ggtgattatg ccagcggaac gaaccacgtc ctgccaaccg gaggtgcggc
     1081 gcgcagccgt ggcggccttt cggtttcgga tttcgtgaag tgcatcagtg tgcaggaagt
     1141 gtcgcgcgct ggcgtggcgc gcctcgcgcc agtggtggcc gaatttgcac gagcggaaaa
     1201 cttggaagcg catgcccgct ccgtggagat gcgaaagtga ctcgggcaat gcctgtgcgt
     1261 cgcgctgtgg agcagatgcg tccgtaccat cctccacttg agggacgcat cgataaactg
     1321 cgcttggatt tcaacgagaa tccgattggc tgctcgccgg ccgttcggcg cgcgctggca
     1381 aagcttagcg ccgcatcaat ttcagcatat cccgaacagg agactgtccg gcgaaaggcg
     1441 gccaggcatt tcggggttcg acccgaggag cttctgctaa caaacggaac ggatgaggcg
     1501 ctcagcctgg tcgtgaacac atttgtcgag ccaaaagacg atattctgct cgtcgagcct
     1561 acctacgcga tgtaccggtt ctattccgaa ttggcgggag cgagaatcgt cgcgccgcga
     1621 tacgacgagg cgatgtgttt cccgtggaac aaagtgctgc aggcgttgcg cgccgggccg
     1681 cgagttttct ttctgcctaa tccgaatagt cccaccggca atctgttaac actcaaagag
     1741 ttgcagagaa tattgaaagc cgcgatgcgc acgatgctcg tgatcgacga ggcctatttt
     1801 gaattttcgg gcgttaccgt gattccttgg atcgggcgcc acaaaaactt gatcgtcacg
     1861 cggacatttt cgaaggctgc aggaatggcc ggattgcgcc tgggctgcat ttttgttcat
     1921 cgcgaattag cttcgattat gcgcaaggct caatcgccgt atcccgtgaa tgctgccgcg
     1981 ctcgcggctg cggaagcggc aatgcgcgac cgggcgttca tcgcgcggac cgtccgtgaa
     2041 gtccgccgcg cccggaaaga actcgagcac ggcctcgctc gactggaagt gcgctgcttt
     2101 cggagcggcg gcaatttcct gctcgtattt ttcggcccgc gtgccaagga aatcgttcga
     2161 gctttgacgc gcaaaggaac cctggtccgt gatcgttcga gcgatttcgg tggtgaaggc
     2221 tatgtccgaa tcacgctagg cacgctccaa cagacccggc gcctgctgcg cgagttggaa
     2281 tcgattctat gagaaaagca aacttgcgca ggaagacaaa ggaaacggac attggcctgc
     2341 gcctgaatct cgatggccgc ggcaagtcgc gcatcagcac gggcatccgt ttcttcgatc
     2401 acatgctcga gcaaatcgcg cggcatggcg cgctggatct gcagcttgtc gcgcgcggcg
     2461 acctcgatgt cgatcagcac cacaccgtcg aagacgtggg aatcgcgctc ggggaaggcg
     2521 tcaagaaagc cctcggttcg aagcgtggaa ttctgcgcgc gggctatttc ctgatgccga
     2581 tggacgattg cctcgcggct gcagcgctcg atttttctgg ccgcgcgttt tgcatgtgca
     2641 gtttcaaaat ttcggcggcg cgcgtgggcg atttccaaac ggagttgatg gaggatttct
     2701 tccgcgcctt cgcgcaggcc gcttcggcga acgtgcatct gaagctggtt tatggcagat
     2761 catcgcatca tcaggtggag gccattttta aggcgtttgc gaaggccctg cgttttgccg
     2821 ttacgcgcga taagcagttg aggcgtgtgc tgccatcgac caaaggtgtg ctatgaaagt
     2881 tgccatcgtc gattatggag cgggaaatct tccatcggtc gaacgcgcgc tgcgaggcct
     2941 gggtgcagaa accgagcggg cgactgaacc tagccagatt gccgctgcaa aggcgatcgt
     3001 gctgcctgga gtgggacatt tttcagcttt tgtaaacggc ctgcgcgaac gcaacctcac
     3061 accctcattg cgtgaggcat acgacttcgg cgtgccgatc ctcggaatct gtctcggatt
     3121 gcaggcgatg ttcgcgtcaa gcgaggaggc gccgggcgcg ccgggcctcg gcttttttcc
     3181 ggaagaagtt cgcgcattgc cgacgaacgt aaagtctccg cacatcggtt ggaatcagct
     3241 gcgccggatg gaagacagcg cattgctgca tggcatccct gaggatgcgt atttttattt
     3301 tgcacattcc tatgcagcac ccgcttccgc tgggcccacg gttgccgcgt gcgaccacgg
     3361 atttccgttc gcggtcgtca ttgaacagaa aaggctggct gccgtgcagt ttcatcccga
     3421 aaaatccggc gagaccggcg ctcgcgtgct gcgcaatttt ctggactccg cgcaatgacg
     3481 ctcgctcgca ggatcattcc ctgtctcgac gtggatggcg gtcgtgtcgt aaaaggaatt
     3541 gagtttgagg gcctgcgcga tatgggcgat ccggctgagt tgggcgctcg ctacaatcgc
     3601 gaaggcgcgg acgaacttgt gttgctggac atcagcgcat ctgtccaggg gcgcgcaact
     3661 tttctcgaaa caattcggcg tgtagcggaa aagttgaata tccccctcac ggccggcggc
     3721 ggcatccgca aaatggagga tggccgcgcg gtcttgcgcg caggtgcgga taaagtgacg
     3781 gtgaacacgg ccgcagtcga gcgcccggaa ttgctttcgg agctggcgca acagttcggc
     3841 gcgcaggcgg tggtcctctc gatggacgtc aagcgcaaaa gcgactcctg ggaaccctac
     3901 acgcgcggtg gccgcgatgc gaccgggctg gacgcggcag tttgggccgc agaagccgtc
     3961 tcgcgcggcg ccggtgaaat tttgctgact tcaatcgacc gcgacggcac ctgcagcggg
     4021 ttcgattgtg aacttactgc caaaatcgcg gcgaccgttt ccgttccggt aatcgcttcc
     4081 ggcggcgcca atacattcga cgattttctc gcggtcttca accaaggcag cgcggatgca
     4141 gccctagccg cttcgatttt ccacagcgag acgcattcca ttcgtgcgct gaagcaatat
     4201 ctcggcgcgc gcggggtgcc cgtccgtctc agccgatgaa ataatcatcc gatgatcatt
     4261 ccctgcatcg atcttcaagg cggcaaagct gtgcagttaa tcagaggacg ccggcgggcg
     4321 ctcgccgtga gcgatgttct aggcttgctc gaacgattcc gcgactatcc catcttgcat
     4381 gtgattgacc tcgacgccgc catgcaaaag ggttccaaca gtcgttggat caagctcctt
     4441 tgccgccgcg caaggatgaa ggtccgggtc ggcggtggca tccgcacggt agcgcgcgcg
     4501 gaaaaaatac tctcgtgggg cgcggagaaa gtcatcatcg gcagcgcggc cttccgaaat
     4561 ggacgtgttg accgcgattt tttgaaagcg atggtctcga gcgtcgggtg caagcgcgta
     4621 attatcgcta tcgacaccga gggagggcaa attgtcgtgc gtggctggcg cgagcgcctg
     4681 aaactcaagc cgagcgatgt gattccggag ttggaacctt tctgctccga gtttctttcc
     4741 acatttgtcg ataacgaagg aacgatgaag ggtacagacc tccgctggtt tcgaaaactt
     4801 cgctccgtca cgtcgatgcc catcactgcc gcaggaggaa ttcgcagcat gcgcgaggta
     4861 cgcgctctgg aaaaaaccgg tatgcacgcc gccgtcggaa tggcaattta cacgggcaag
     4921 ctcgcaggag acaagcgggc cctcggcggc cctatgcttt cgcccacctc ccttaaatag
     4981 cttgccagaa atagtttgcc agcgcgtcgt gcggcggcag gaacagccac agcagcggcc
     5041 gtcgcgcaac ttcgcttgct ggtgtatagt tttttgtggt gcggcatcac agacggcggt
     5101 catttcccac ctcgggataa ccccggtcgc tgtgcgccgt acttcctgga gcatgaaaac
     5161 acaattgcga gtgatgaaat ttggcggcac gtccgtgggc gacgcgtcgt gtatcaatcg
     5221 tgtcgcccaa gtcgtgaagg aagcggcgcg cgaatgccca attgtggtcg tggtttcagc
     5281 catgagcggc gtcaccaatt ggctgatcca agcggcgaac cgttccgaag ctggagatcg
     5341 cgaacattcc cacgcgattt tcgcagagct gcgcaaacag cacgcggcag ctctggaaac
     5401 gcttgttcgc gataaggaaa agcgccgtcg agtagtgagc tgcctcgaaa aacttttcaa
     5461 cgagggcgag gagctttgca agggcacggc cttgcttcgc gaactcactc cgcgaacgct
     5521 ggattccata tcaagtttag gtgagcgcct ttcggctccg atggtcgcgg cggcattgtc
     5581 agagcacgac gtgccaaccg aggccgtcga atctacggag ctaatcgtca ccgattccta
     5641 tcatggcggc gcggacccgt tgatggaccg cactcacgcc cggtcgaacg cacgcttgcg
     5701 gccgttgctc gaacgcggaa ttgtaccggt ggtgacaggt ttcattgggg ccaccgatag
     5761 cggcgttcta acaacgctcg ggcgcggcgg atcggattat tccgccacga ttctgggcgc
     5821 agctcttagc gccgacgagg taatcatttg gaccgatgtg aacggagtgc taacggccga
     5881 cccacgcctc gtgccggaag cgcgcactat ccccgaaatt tcctatctcg aagcatccga
     5941 gctcgcttat tttggcgcga aggtgcttca tccgaagacg cttcgcgccg tggcgcaggc
     6001 gggcattccc gtttggattc gaaacacctt cgcgcccgag caggctggca cgaagatcac
     6061 gcccaggggt cgcagtaatg gcggcggagt gaaggcgctc acagctattc gcgatgtggc
     6121 gctgattacc gtcggcggcc cgggaatcgt cggtgtgccg gatgtcgttg gccgtacgtt
     6181 ttccaccacc gccgaagtac acgcgagcat cctgctgatt tcgcagtcgt catcgcaaaa
     6241 cgacatctgt ctcatcgtcg ccgcctccga tgcgaagcgg actgtcgagg ctctccgcaa
     6301 ggaatttgcg caagatgtgg cgcacgaaaa agtggaacac atcaccatgg atccaagcat
     6361 cgcaatcgta gccgtcgtgg gcgaaaacat gcgcggtacc ccaggcgtcg cgggacgcac
     6421 tttcaacgcc gtggggcgcg agaatgtcaa catcatcgcc atcgcgcagg gctcgtccga
     6481 gagcaacatt tctttcgtcg tctccgagaa agatatgaag gtggcgcttc aggccactca
     6541 ccgtgaattc aatttgagcg cgtcggttcc tttcgaatcg gaagtgaaag tttcctgaga
     6601 tgaccgaaaa gaccgctcgc gcgccggaaa tcatcaccga aaaggctgtg cagcgctgca
     6661 tcgacccggc ttgcggcgca acatacgacc tgaacgagcg catttatctg tgctcccgct
     6721 gcggcggcac tcttgagatc agccaaaact tatccgaaat cggcgacgcc accgctttgc
     6781 gcaagcggtg gtcggctcgc gcggcctcgc gcgatccgcg cgacgccagc ggtgtgtggc
     6841 gttatcgcga gcttcttccc tttgatgacg ccgcgccttt cgtaactctc ttcgagggta
     6901 atacgccgct gtatcacggc ccgcgttccg cccgttactg cggattggat gacttgcgtc
     6961 tcaaacatca ggggtgcaat ccaaccggct ccttcaagga caccgggatg accaccgccg
     7021 tcacgcaggc tgtcgttctc ggcgcgcgaa ccgttgtctg cgcgagcacc ggaaatactt
     7081 ccgccagtct cgccgcctat gcaggccgcg ctggattgcg agccggcgtc ctacttccgc
     7141 gcgggcaaat ttcttcggga aagctcgcac agagtctcga ctacggcgct ctcgtatgcg
     7201 aaattgacgg gaattttgac gattgcatgc gcctgattca ggaattgggc gaggatcctt
     7261 ccatctacgt cgcgaattcg ataaatccat ttcgcatcga ggggcaaaaa acggtcgcct
     7321 tcgagatgat ggagcagtgc gcgtggcagg ttcccgacca cgtcgtcgtt cccggcggca
     7381 atatgggcaa cagcaccgcg atcggcaagg gttttcaaga actgctcgac ctcggtttga
     7441 ttcatcgcct tccgaaactc agcgcgattc aggcggaagg cgctgcgccc gttgcaaatt
     7501 tgtttgccaa attgaaggcc gatcaattaa cgggcgaaat gccgctgcct gaagttatgg
     7561 agacggtcga gaatccgcgc accctggcga ccgctatcaa aatcggcgcg cctgtttcgt
     7621 ggaagaaggc ccttcgcgct attttgcgct cgggcggaaa agttatcgcg gttagcgaac
     7681 aggaaatcgc cgatgccaaa gcaatgatcg gccgcgatgg aatcggctgc gagcccggtt
     7741 cggccacaac cgtcgcggga atcaagaagc tcgtcgccgc gaaacatatc cgcagccacg
     7801 attccgtgtt ggcggtgctg accggaaaca tgctgaagga ccccagctac gtgagccatt
     7861 atcacagcgg aacgctcgtt ctggactcgc cgggtggtcc cccgcagcgc attgtgggtt
     7921 cattccaaaa cgccccgaaa acggtagccg ccacaaaagc cgcgattctc gcggcgttag
     7981 cgcaagccgg caaggaaggt aatttgccgt gaggaacact tcgcgcgcct gagccgtttt
     8041 cgccatgatc ctatgacttc tgcccgacaa ccgtcattcg aggtgcgcgt tcccgcctcg
     8101 accgccaaca tgggagccgg cctcgattgc ctcggacttg ctctcgaaat gtacctcagc
     8161 gttcgcgcca ccgtcctctc gaaacccggc tcgcgcacgc tggcgcggag tcgtggtgtg
     8221 cgcggcactt ccgaccttcc tcgcgacccc gaagaaaatc tcatctttcg tgccatgcgc
     8281 catactgtcg aacgtgaagg cttcgttctg cctcgcgtcc ggctcgccgt tcaaaacgaa
     8341 atacctgtcg cgggagggct cggcagcagc gcggccgcaa cggtagcggg cgtagctctt
     8401 ggcttcgcgg tcgccgggcg gcaagtgccg aaagacgtag cgttgcgtta tgccgcggag
     8461 atggaggggc acccggataa cgtcgcggcg gcccttcttg gcgggttggt cgtcacattc
     8521 actcgtagtg atggcacagt ggccgcggtt cgcaggcact ggcccaaagt gattcgattg
     8581 atcgtagtta ctccgaatat gaagctggaa acaaaaaaat cgcgcgccgc gctgcccgca
     8641 accgtctctc gagcggatgc ggtgcacaac ctgcagcgct ccgccctgtt tgtagcggcg
     8701 ctcgaagacc gtcgctatga ccttctctgg gatgccatgc aggatcgcct gcatcaaacc
     8761 ccgcgccagt ccttgattcc gggtctcgcg gaggccctgg cccttcctcg catgcctggt
     8821 ttgttgggtg ttgctctaag cggagcgggc cccagcgtta tggccctggc cacgggccgc
     8881 cacgatgaaa tagggaaagc tatcgctcag tgctttgagc ggcacgatct cgcgcccacg
     8941 attcgaaaca tggaagccgc tcaggagggg ctggtgtcca cgcagaagta tatctcgtgg
     9001 aagtaaccgc acctctcctc acacacgttc aagctctgtg aacctaaatt tagttctgga
     9061 ctctacgacg gaagtatcca aaccagccgc tcgccagcac gatggatatt cctgcggcag
     9121 tcagcacgaa gctgtcgggc tccggcgtcg cggccggagg aatcgttggc ggtgtggtcg
     9181 gaggaatgat cgtgttgtgg gatgaagagc ctcctcctga gatcgggaaa acgggagaat
     9241 tgtccgcacc gcccccgaaa tctgagcttg gtaccagcga agacgaaaaa gcgggaaacg
     9301 gcagtgccat cggcaaagca atcggccgag cttcggccag gtcggggaga ggtgctggat
     9361 cgagaatcat gtcgagcggc tgcttctccg caacggggga gcgagccgta gccgaaaggc
     9421 ggtttccgca gcgcgtccgg gccgtattct tgccgtctgt aattagggtc tggcccctca
     9481 aaagttttag tttcttcttg gtccaataga tacggttgtt caggcgatag gagacgtaca
     9541 tttcctcatc gctgtctagc cgtataacat gagtcttact aacgtcgaaa ccggcgtagt
     9601 gcgcggcggc gattgggtcg tgttctagcg ccttgcgcaa ttcttccgcg ctttccactc
     9661 ctccaggaat caccgaatag ggaaagaggc gtcgcgaacc tgccactgcg tcaaggggcg
     9721 aatcagatgt gtctggtcgt gccggcgtga cgaaagattt tgccgcgttt gctgtctgcg
     9781 ctcgttcctg cttcgcgcta tttcgttcgg tgacaaccgt accccaaaac gtcaccgaaa
     9841 atagacacat gagtgtaacc aaatacaccc aaccgaccac gcttccccgt cgaatcaaaa
     9901 tatcctcgat ccgctacaac ctagccgacg cattgtcctc agtcgatgac aactagttgc
     9961 tctggatcaa gcaattctca tgccagcagg gcgaatcgat cccttgggcc cgtattagcg
    10021 tctttttttg aatctcacgc tgcaagggaa gctttctgac acacggagga ggattgtcct
    10081 ccgtttttac ggaaaattat tcggcatttg ggagtttatc gggaagcagt acggaagttc
    10141 tgatacctcg ctgcacatcg gcggaaggtc tttcgaaaaa aacaaaaaaa tcccgtttca
    10201 ggaaatattg atcggcgcgc tcgcaaaggc cgcgctactc gatcaccagg cgcagaaatt
    10261 ttttcttccc ggcacgcaga agatattcgc cgggcttcga gagatcgatc tcgcgtttca
    10321 catcgtccac acgcgcgccc tcaagctcca cgccgccctg cttcataagt cgctccgcct
    10381 cgctgcggga cgatgcgagc tccaactcca cgagcaacac cgacagacgc tttggcgcgc
    10441 cctgtggaat cgtgtgtcgt ggcgcttcgg caggtgcttg gcgctcgcga aacacccggc
    10501 gaaatttatc ggacgctcgc cttgccgctt cctctccgtg aaagccggcg atcaccgtgt
    10561 gcgccaattg cattttcgcg tccatcgggt ggagcacgcc catgcgaact tcctcccgaa
    10621 ggcgcacgat cacaggcggc ggaaaatccg tcagcagctc gtaatacgac cacatcaagt
    10681 cgtccgaaat agacatcagc tttccgaaca tttctgccgg cgcttcggtg atgcccacgt
    10741 agtttcccaa actcttggac atcttgcgtt cgccatccag tcccaccaag atcggcatgg
    10801 tcagcacagc ctgagatgtc tgcccgtatt cgcgctgtat ttcgcggtgc accaggagat
    10861 taaacttctg ctcggtggct cccagctcca catccgattt cagcgccacc gcgtcgtagg
    10921 cggtcagtag cggatacagc aattcgtgga cggagatcgg ctggttcttt tccagtcgcg
    10981 aacgaaaatc ctcgcgctcg agcatccgcg ccagccggta gtggccgcac agccgcaccg
    11041 tatcgtagct cgtcattttg gccagccatt cgctgttgaa gcgcacctcg gtcttctcgc
    11101 ggtccaggat cttgtacacc tgctcgagat aagttttcgc gtttgcatcc acctgctctc
    11161 gcgtcagcgg cggacgtgtt tctgattgcc ccgtcgggtc gccgatcatc gccgagaaat
    11221 cgccgatcag gaaaatcgcc gtgtggccca tgtcctgaaa atgcttcagc ttccggagga
    11281 caaccgtatg gcccagatga atgtcggggg ctgtcgggtc cacaccgaga tatacgcgca
    11341 gcggcttgcc ggacttggcg gactgatcga gcttcgcctt cagctcctct tcgcggataa
    11401 tctccgccgc gccctttctc aggtacgcga cctgttcctc aaccggtttg aattgcgtag
    11461 gtgtcaccta tgttcccccg gcccgttccc gccgccgtcg accaaaacgc ggcggccgac
    11521 gatgcggcat cgcccttcga gcaccatgcg cacggcttcc gtataaacgc gatgttcctc
    11581 tcgtaaaatc cgctcggcga gcgtagcggg cgtgtcgtcg tcttccaccg gcaccaccgc
    11641 ttgcaggatg atcggtcccg cgtccaggtt ttcgtccacg aagtgcaccg tgcagcccga
    11701 gaatttcacc ccatggtcaa tcgcctgcct ctgaggctcg agtcca
//
LOCUS       DATNDT010000264         3086 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3169395, whole genome shotgun sequence.
ACCESSION   DATNDT010000264 DATNDT010000000
VERSION     DATNDT010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3169395"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /gene="gltX"
                     /locus_tag="VN788_08525"
     CDS             complement(<1..1282)
                     /gene="gltX"
                     /locus_tag="VN788_08525"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HXU20612.1"
                     /translation="MALNSPVRVRFAPSPTGHLHVGNVRTALFNWLFARQNDGTMVLR
                     IEDTDVERSEARFESMLMDDLKWLGLDWDEGPDTGGEYGPYRQSDRLEIYREHAERLL
                     TDGKAYLCFCTEEELQKERESSSAEKGQAIYSGKCRRLDPQEAKSRRSNGETCAVRLK
                     IPERPIRFHDIVRGDVEFSNEVVSDPVIVRSSGMPVYNYVVVIDDALMKITHVIRGDD
                     HLSNTPKQVALYEALGWPVPEFAHLSTILGADRERLSKRHGATSIANFREMGVLPEAL
                     MNYLALLGWAPTGGTREIFSAKELIKEFLLKRVTPSPAVFDLEKLYWLNRHYIKEASP
                     ERIEKLALPYYSRTLAEEVAEDLGQQEKIPVPDSHWPPAIVGWFASVTRLLSPYVDRL
                     EQLPERAKPILDYDAKAAISSQDNAEAFAWPHTDA"
     gene            complement(1396..2562)
                     /locus_tag="VN788_08530"
     CDS             complement(1396..2562)
                     /locus_tag="VN788_08530"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXU20613.1"
                     /translation="MNHNSQPDYTNSPEAEFVDPEPFDASEQQFAASLDVPAGQQANA
                     VDRDDAGNAAPEHNPSPCAATLSSTITEEASEDSVKQGNGADASSNDSSASSTWRKEV
                     ADRVSKYRRKSPREPRYPSLQLKFESSLPAWTARTPESVARQAVALERNPVPIAGAIS
                     EASPRSTTFAEPASDPEYASNLIEFPRFTAERPSHVDELAEPVLDRLRILEAPEVAPS
                     PPALGGILIEQAEERPQERRPGFEIPLQSSSLSRRMAAAIIDAVIVLASLGVFGYVFS
                     RLTDPTLRLFQWMEMAAALAGVFWVAYQYSFLVYSGNTPGLRIAGLRLARFDGADASR
                     QLRRWRVLSSLLSAGSIGLGYLWCFLDEDQLCWHDRITRTHLAPKSAPEKSTQT"
     gene            2710..>3086
                     /locus_tag="VN788_08535"
     CDS             2710..>3086
                     /locus_tag="VN788_08535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20614.1"
                     /translation="MLTSAYLPALRRIPASAMNFRAQLLFSVPVIAGHLLLLSFLCIS
                     QARAQTIPDISGAIGTTRNDDVSIQALSQEKQGTMYKLKGNVEIHYGVFILRADEVTY
                     DSDTKQSHAIGHLVLEGGPADEH"
BASE COUNT          648 a          829 c          933 g          676 t
ORIGIN      
        1 ccgcatcggt atgcggccat gcgaatgctt cggcgttgtc ctgtgagcta atcgcggcct
       61 tggcgtcgta gtcgagaatg ggcttggccc gctccggaag ctgctccagt cggtcaacat
      121 aaggagaaag aagtcgcgta acacttgcaa accatccgac aatggcggga ggccagtgag
      181 aatcgggcac gggaatcttc tcctgctgcc caaggtcttc ggcgacctct tcggcaagcg
      241 tgcggctgta gtaaggcagc gcaagctttt caatgcgctc cggcgaggct tctttgatgt
      301 aatgccggtt cagccaatag agcttctcca agtcgaaaac ggccggagaa ggagtgacgc
      361 gcttcagcaa aaactctttg atcaattcct tcgcgctgaa aatttcgcga gtgccgcccg
      421 tgggtgccca gccaagcagc gcgagataat tcatcagcgc ctccggcagc acgcccattt
      481 cgcggaagtt cgctatggaa gttgccccat gcctcttcga gagcctttcc cgatcggcgc
      541 ctagaatggt ggaaaggtgc gcgaactccg gcaccggcca gcccagggct tcgtaaaggg
      601 caacctgctt gggagtgttc gagagatgat cgtcgccgcg gatgacatgc gtgatcttca
      661 taagcgcgtc atcaattacg acaacgtagt tgtaaacagg cattccgctg gagcggacaa
      721 tcaccggatc gctgacgact tcattggaaa actcgacatc tccgcggacg atgtcgtgaa
      781 agcggatggg gcgttcgggg atttttaacc ggacggcgca ggtctcgccg ttactgcgac
      841 gcgacttagc ttcttgcgga tcaaggcggc ggcattttcc tgagtagatc gcctgtcctt
      901 tttcggcgga cgagctctcg cgctccttct gcaattcttc ctcggtgcag aaacataaat
      961 atgctttgcc atcagttaac agtcgctcgg cgtgttcacg gtaaatctcc agcctgtcgg
     1021 actggcgata agggccatac tcaccgcctg tgtctggacc ctcgtcccaa tccagaccga
     1081 gccatttgag gtcgtccatc agcatggatt caaagcgggc ttcgctgcgc tctacgtcgg
     1141 tatcttcaat gcgaaggacc attgtgccgt cgttctggcg ggcgaagagc cagttaaaga
     1201 gcgcggtgcg gacattgccg acgtgcagat gtccggttgg ggatggagca aaacgtacgc
     1261 gaacagggga gtttagggcc acgaattatg ttatcagagc gtggcgggga ctctttcaga
     1321 gtgtggtgac ggtagggatg cttcgactcg cgagagctgc gctgcgcttg ctcttgcttc
     1381 gctcagcatg gcaagtcagg tttgggttga tttttcggga gcggattttg gagctaaatg
     1441 cgttcgggta atgcggtcgt gccagcatag ttggtcttca tccaggaagc accagagata
     1501 tccaaggccg attgagccgg cggagaggag cgagctgaga actcgccatc ggcggagttg
     1561 ccgtgaagcg tcagcgccgt caaagcgcgc gaggcgaagg cctgcgatcc gcagccctgg
     1621 ggtgttgccg ctgtagacca gaaatgaata ctgataggcg acccagaaca cgcctgccag
     1681 tgcggccgcc atttccatcc attggaacaa acgcaaggtc ggatcggtca ggcgggaaaa
     1741 tacgtatccg aatactccaa gcgaagccag cacgatgacg gcgtcaatga ttgctgcggc
     1801 catgcgccgg ctgagggacg acgactgcaa agggatctcg aagccgggac gcctttcctg
     1861 gggacgctct tcggcctgct cgatcaatat tccgcccagc gccggcggcg aaggagcaac
     1921 ttcaggcgct tccagaatgc gcaaccggtc gagcaccggt tcggcgagct cgtccacatg
     1981 gctggggcgc tccgcggtaa agcgagggaa ctcgatgagg ttgctggcgt attccggatc
     2041 gcttgcgggc tcggcaaagg ttgtggatcg tggcgaagct tctgaaatcg ccccagcgat
     2101 ggggactgga tttcgttcca aggccacggc ttgccgcgcg acggactctg gcgtccgggc
     2161 cgtccacgca ggcaggcttg attcaaattt caattgcagt gacggatatc gaggttcgcg
     2221 gggactcttg cggcgatatt tgcttacgcg atcggcgact tcttttctcc aagtggagga
     2281 agcagaagag tcattggagg aggcatccgc gccgttacct tgttttacgg aatcttcgct
     2341 cgcctcttcg gttatcgtgg aggagagtgt ggcggcacaa ggcgaaggat tgtgctcagg
     2401 agcggcattc ccggcatcat cacgatcgac cgcgttcgct tgttggcccg cagggacatc
     2461 gaggcttgcg gcaaattgct gttcgctggc atcgaacggt tcaggatcca caaattcggc
     2521 ttcgggggaa ttcgtgtagt caggttggga attgtgattc atgaagacgg actacctaaa
     2581 tgtatacccg caaccggtgg acgcacatgt tagaaaacta cgcgaggcga aaaaactgac
     2641 gtacgtgtat ctaaagtatc aagtgctcca gcaaattcca gcgccatctt tgtctcccgg
     2701 gatgatggca tgctaacgtc agcatacctc ccggcattgc ggaggattcc tgcgtccgcg
     2761 atgaacttcc gcgcacagct tctttttagc gtcccggtca ttgccggcca tctgctgctg
     2821 ctttcttttt tgtgcatttc tcaggcgcga gcgcagacca tccctgatat ttccggagcg
     2881 attggcacta cgcgcaatga cgatgtttcc atccaggccc tttcccagga aaaacagggg
     2941 accatgtaca agctaaaggg caatgtggag atccactacg gagtttttat tcttcgcgcc
     3001 gacgaggtca cgtatgactc cgacactaaa cagtctcacg ccatcggcca tctggtgctc
     3061 gaaggtgggc cggcggacga gcatat
//
LOCUS       DATNDT010000265         3178 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2907936, whole genome shotgun sequence.
ACCESSION   DATNDT010000265 DATNDT010000000
VERSION     DATNDT010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2907936"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1709)
                     /locus_tag="VN788_08540"
     CDS             complement(<1..1709)
                     /locus_tag="VN788_08540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20615.1"
                     /translation="MRFPSASSRSTKLLLAILAAAASVVCLYYPFFFSRGNLVSGDLV
                     DNRLYIVLLEHWRAVFSGHAAMTSPNFFAPYPGALGLSESLFLYTPFYLAFSFLGGDP
                     ALSFQLTLIALRLIGFFAVLALLRRGLKLSFIASLFGACLFTISNIYFVTEVHGVFAA
                     VAFLPLFGVLLLGYVRRGSRACLWISAALAGLLFFTCYYMAFLGALAGIVFLLCSWPR
                     VNKLQSRHVIRDLTVAALLFAIALIPFFLTYWPEFRSTGGRSYGESLVYARDWRELFD
                     TGSKNMIWGKFDSAIYDAIRRPELEYGRGWPPVTMLTASILFLIALFRKQLARRRIAI
                     SIGATCALLYFVSVKYGTWSAWWIVYRLVPGSTGIRVPGRINNVINLGVVALCAIAIH
                     WLWSGTRKRKLTAIALGLFLLIEQVNRAPLAMINRTVERSTFARIAPAPAFCKTFFAI
                     DTRRPDDVGQVDAMLLARSSGIPTINGHSGWAPDGWDLHRFDADYPFEARTYALEHHL
                     QSGFCNVDFSSGQWKLVDTTDGNRYQIGTEITFGAAGNSGKYKGGGWSYAEQAASWTR
                     SPDA"
     gene            complement(1793..1870)
                     /locus_tag="VN788_08545"
     tRNA            complement(1793..1870)
                     /locus_tag="VN788_08545"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1833..1835),aa:Pro,seq:ggg)
     gene            2054..>3178
                     /locus_tag="VN788_08550"
     CDS             2054..>3178
                     /locus_tag="VN788_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU20616.1"
                     /translation="MALAAAPAFVPVLGANKKITIGWIGVGSRGYYLMERLYKGSPDL
                     AQVTAVCDTYADHLTRAKDRVQTMGGNSPKTYTDYREMLQDPSIDAVFISTPEHLHYQ
                     MFMDAIKAGKNIYCEKPLAHTIEQGEQMVKAAEASGKVVQVGTQNRSNSLYIKAKEMV
                     EQGMIGDIHYVRAYWYRNSLDNNPAWRYEIPADANPDNTDWERFLGPAPQRPFSKERY
                     YQWRLYWDYSGGISTDLLVHQTDITNFVCGKVVPSTCVASGGIYRWTDDDREVPDTFS
                     ALYEYSERFHINYSSYFGNDHYGYGENFMGNEGTIEVLNRQTLNFYPEKFRGQAPPKV
                     AARQELHIDLPGNDNKAVEAHIHNFLEAIDGKAKIVAPTRT"
BASE COUNT          805 a          931 c          861 g          581 t
ORIGIN      
        1 gcgtcgggac ttcgtgtcca ggaggcagcc tgttcggcat aactccagcc gccgcctttg
       61 tatttacccg agtttccagc cgcgccaaaa gtaatttccg taccgatttg ataccggtta
      121 ccatccgtgg tatctactaa cttccattga ccgcttgaga aatccacatt gcagaatccc
      181 gattgtaagt ggtgttcgag cgcgtatgtt ctcgcctcga acggataatc ggcgtcgaat
      241 cgatgcaaat cccagccgtc cggcgcccat ccgctgtgac cgttgatagt ggggatgccg
      301 ctggagcgcg ctaacagcat cgcatctacc tgtccaacgt catcggggcg gcgcgtgtcg
      361 atcgcgaaaa acgtcttgca aaacgcaggc gcaggcgcga tgcgagcgaa agtcgaacgt
      421 tcgacggtcc ggttaatcat ggcgagcggc gcgcgattga cctgttcgat caagagaaac
      481 agacccaaag caattgccgt caatttccgc tttcgggtgc cgctccataa ccagtgaatc
      541 gcgatcgcgc agagcgccac cacgcctaga ttgataacgt tgttaatcct tccaggcacg
      601 cgtattccgg tgctcccggg gactaggcga tacacgatcc accaagccga ccatgtgcca
      661 tacttgaccg acacgaaata taaaagggcg caagtggccc cgattgagat cgcgatcctt
      721 cgccgcgcga gttgctttcg aaaaagcgca atcaggaaga gaatgctggc cgtcagcatc
      781 gtgaccggcg gccagccccg accgtattcg agttccggcc ggcgtatcgc atcgtagatc
      841 gccgagtcga acttccccca gatcatgttt tttgaacccg tatcgaaaag ctcacgccaa
      901 tcccgcgcat acaccagcga ttcgccgtat gatcggcctc ctgtgctcct gaattcaggc
      961 caataagtca ggaaaaatgg aattagggca atcgcaaaca gaagcgcggc aacggtcagg
     1021 tcccgtataa catggcgcga ttgtaattta ttaactcgag gccacgaaca gagtaaaaat
     1081 acaatgccag cgagcgctcc caggaacgcc atatagtaac aagtaaaaaa tagtaaccca
     1141 gccagcgccg cgctaatcca caaacacgcc cgggagccac gtcgaacata cccaagcagt
     1201 aagacgccga acagtgggag aaacgccacg gcggcaaaca cgccgtgaac ctccgtcaca
     1261 aagtaaatgt tggaaatcgt gaagaggcag gcgccaaata gagatgcgat aaacgacaac
     1321 ttcaatccgc ggcgcagcaa cgcaagcacc gcgaaaaatc cgatcagacg gagcgcaatc
     1381 agagtcagtt gaaacgacaa agccggatcg ccccccagaa acgagaacgc gagatagaag
     1441 ggtgtgtaga gaaatagaga ctcgcttaaa cccagcgcgc ccggatatgg cgcgaagaaa
     1501 ttgggactgg tcatggccgc gtggccggag aaaactgcgc gccagtgttc gagaagcacg
     1561 atgtataagc ggttatcgac aagatccccg ctgacgagat ttccccggga aaagaagaac
     1621 ggataataga ggcaaaccac gctggccgca gccgccagaa tagccaggag aagcttagtg
     1681 gacctgctgg atgcggatgg gaatctcatt cgcggctcaa gttgtcaggc cgatccgcca
     1741 aaaccagggg gaagaacaaa tgtggatcgg aacggggttg atttgctgaa actggtcggg
     1801 ccgccgggat ttgaacccgg gacctcttgc accccaagca agcgcgctag ccaggctgcg
     1861 ccacggcccg aacgtcttag tgtaccgcac ccctttccag cggcggcgga agtgtcccgg
     1921 agttggtatg ctcagcaatt aaagaaatcg cgcaaaacgg agcgcgggcg ccgcgccctt
     1981 tgcgctttta aggagacagc acccaatgca agatgaactt tcacgccgca gttttgtgag
     2041 gcgcaccgct ggcttggcgc tggcggccgc accggcgttc gtgcccgttc ttggcgcgaa
     2101 caagaagatc accatcggct ggatcggcgt cgggagccgc ggttactacc ttatggagcg
     2161 tctgtacaaa ggtagccccg atcttgccca ggttaccgcc gtctgcgata cctacgccga
     2221 tcacctgacc cgcgcgaaag atcgcgtcca gaccatgggc ggcaattctc ccaagactta
     2281 cacggactac cgtgaaatgc ttcaggaccc ctccatcgat gccgtcttca tttccacgcc
     2341 ggaacatttg cactatcaaa tgttcatgga tgcgatcaag gcgggcaaaa atatctattg
     2401 cgaaaaaccc ctagcgcata ccatcgagca gggcgagcag atggtgaaag cggcggaagc
     2461 gtccggcaaa gtcgtgcagg tcggcacgca aaaccgcagc aattcgctgt acatcaaggc
     2521 gaaggagatg gtggagcaag gcatgatcgg cgacatccat tacgtccgcg cctattggta
     2581 ccgcaattcg ctcgataaca atcccgcctg gcgttacgaa atccccgccg acgcgaatcc
     2641 cgataacacc gactgggagc gattcctcgg acccgcgcca cagcgcccct tcagcaagga
     2701 acgatattat cagtggcgcc tttactggga ttactccggc ggcatctcca ccgacctgct
     2761 ggttcaccag accgacatta ctaatttcgt ctgtggcaag gtggtcccgt cgacatgtgt
     2821 cgcctcgggc ggcatctatc gatggaccga cgacgatcgc gaagtacccg acaccttcag
     2881 cgccctctac gaatactccg agcgcttcca catcaactac agctcgtact tcggcaacga
     2941 tcattacggt tatggcgaga acttcatggg taacgagggg accattgagg tcctcaaccg
     3001 ccagacgctc aatttctatc ccgaaaaatt ccgcggtcag gcacccccga aagtcgccgc
     3061 gcgccaggaa ttgcacatcg atcttccggg caatgacaac aaagccgtgg aggcgcacat
     3121 tcacaatttc ctcgaagcca ttgatgggaa ggccaagatt gtcgcgccca cccgcacg
//
LOCUS       DATNDT010000266         5753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3169758, whole genome shotgun sequence.
ACCESSION   DATNDT010000266 DATNDT010000000
VERSION     DATNDT010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3169758"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..294
                     /locus_tag="VN788_08555"
     CDS             <1..294
                     /locus_tag="VN788_08555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010637454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HXU20617.1"
                     /translation="FTEHGAIMAATVLNSERAVEMSIFVVRAFVRMREVLSSNQKIVS
                     KLGELERRLETHDSDIQQLIEAIRELMTPPEPNRRKIGFEFPSAPAKTHGKKK"
     gene            complement(329..610)
                     /locus_tag="VN788_08560"
     CDS             complement(329..610)
                     /locus_tag="VN788_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20618.1"
                     /translation="MPIFIFAVLINFLRALRTHLRRVRDSKPKSLCAGCFYAHVQYGA
                     NAERAISCTFGGSIRSVKLDVLYCTDYQARTLPGPIRMIGFEREVAQTK"
     gene            complement(673..1983)
                     /locus_tag="VN788_08565"
     CDS             complement(673..1983)
                     /locus_tag="VN788_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TROVE domain-containing protein"
                     /protein_id="HXU20619.1"
                     /translation="MARLNTLNIFGPRTHEGAPARNIPVELQLRRSVLACLLWESQFY
                     EDGVEIAGRIAELVPRVAAEKVAALAVEAREQMKLRHAPLLLAREMARHKTHRALVSE
                     TLARVIQRADELAEFVAIYWKDGRVPLSGQVKKGLAAAFPKFDEYQLAKYDRSGPIKL
                     RDVLFLSHAKPRDVEQASVWKKLIWGKLATPDTWEVALSAGSDKREAWERLLREQKLG
                     ALALLRNLRNMREAQVDESLVLAALGEMNTARVLPFRFLAAARYAPQWEERLEQAMLK
                     SVANAEKLSGKTIVLVDVSGSMTAPLSRRSEMQRTDAAYGLAVLLREIGEKVSVFSFS
                     DKLAQVPARRGFALRDAIDGSQRHNSTQLGAAVGELNRNENYDRLIVITDEQAHDTVP
                     SPKGKGYVINVASYKNGVGYGKWTHIDGWSESVVEYVRMLEHTQ"
     gene            complement(2348..2779)
                     /locus_tag="VN788_08570"
     CDS             complement(2348..2779)
                     /locus_tag="VN788_08570"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU20620.1"
                     /translation="MKAGDARPSQNLKQPNGPGRGEEISSLLGVTLSGLLNVLDGFNA
                     PEDILFVMTTNKIETLDLAFLRPGRIDYRLFLGKAASSQKISLYRRFFPEATDAEARE
                     FVDAHESTQTMAEFQGLLLALEQEAKDPESVATDESHGILV"
     gene            complement(2803..3426)
                     /locus_tag="VN788_08575"
     CDS             complement(2803..3426)
                     /locus_tag="VN788_08575"
                     /inference="COORDINATES: protein motif:HMM:NF020322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU20621.1"
                     /translation="MWVKEWFLEQEFLKRIRRLDLDTTLRGEGSALIPAPGRHRFWYE
                     RRPFWVWFSRTEETKGWTPRRLESLTFRTVGREQWFLKRFVDEIVACHKKKIRLASYL
                     YVYDDGWIRVLGYTPRMLEAVIPKPGEKEHLLEDIRRFRAARKRYCELGVPYHRGYLL
                     YGPPGTGKTSLVSGFAATFGMSVYAINLTEFNDRTLKSAVSDVPAPR"
     gene            complement(3440..3607)
                     /locus_tag="VN788_08580"
     CDS             complement(3440..3607)
                     /locus_tag="VN788_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20622.1"
                     /translation="MKEFLKYVLNGQNQFASGGLLLMIIGGISVYLRALPTKLWQWII
                     EQSTLTLTVKE"
     gene            4330..4527
                     /locus_tag="VN788_08585"
     CDS             4330..4527
                     /locus_tag="VN788_08585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20623.1"
                     /translation="MKMLEDATAYNFLQEQKIVVVAQFAKYATPENQVISTLSEAKTG
                     ETLRALKAINTDNKDRSCCQH"
     gene            4731..5522
                     /locus_tag="VN788_08590"
     CDS             4731..5522
                     /locus_tag="VN788_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU20624.1"
                     /translation="MSAIRVENLEKTFGDLTAVNKISFQVEKGEIFAFLGPNGAGKTT
                     TIKMLTTLLNPTSGKIELDGLDPTIHRNEARQRFGIVFQDPSLDAEQTAYENMELHGI
                     LYKVPHKLRVQRTEELLKLFELWDRRDDYVKTFSGGMKRRLEIARGFLHTPKILFLDE
                     PTLGLDPQTRNQLWTHVKSVNESENTTVFLTTHYMDEADRVAHRIAIIDHGAIVAMGT
                     PQELKQQTNSESLEQAFLALTGSTIRDESATSQDQLRRFAQMWRK"
     gene            5554..>5753
                     /locus_tag="VN788_08595"
     CDS             5554..>5753
                     /locus_tag="VN788_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HXU20625.1"
                     /translation="MSAIYILWLRELKRYIRSRAQIAASLGQPLLYLLALGFGLGPVF
                     QKAGQGSYFQFVAPGVVSMTIL"
BASE COUNT         1365 a         1731 c         1429 g         1228 t
ORIGIN      
        1 ttcaccgagc acggagccat catggccgcc accgtcctga attccgaacg cgcggtggaa
       61 atgagcatct tcgttgtccg cgccttcgtc cgcatgcgcg aggttctgtc ctcgaatcaa
      121 aagatcgttt caaaattagg agaacttgaa cgccgcctgg aaacccacga ttccgacatt
      181 cagcaattga tcgaagcaat ccgcgagttg atgacgccgc ctgagccgaa ccgccgcaag
      241 attggattcg aatttccctc cgcacccgcg aaaactcacg gcaaaaagaa atgaggcgga
      301 ttctccgcct cgtttctcgc tgtcatctct acttcgtttg tgcaacttcg cgttcgaagc
      361 caatcatgcg aatcggccca ggcaaggtcc gcgcctggta gtcggtgcag tacaacacgt
      421 ccagcttcac cgaacgtatt gagcctccga acgtgcagga gatcgcacgc tcagcatttg
      481 cgccgtactg cacgtgagcg tagaagcatc cagcgcaaag ggacttcggc tttgaatcgc
      541 ggactcttcg caagtgagta cgcaaagctc gcaaaaagtt aataagcact gcgaaaatga
      601 aaatgggcat gcgacctcct ttcaatgatt tgcttcatat gcgggacggg ctctcgcccg
      661 ccccagattt acctactgcg tgtgctccag catccgtaca tactcgacca ctgattcact
      721 ccagccgtca atgtgcgtcc acttgccgta gcccacaccg ttcttgtaac tcgctacgtt
      781 gatcacgtaa cccttgccct ttggcgaagg caccgtgtcg tgcgcctgct cgtcggtgat
      841 cacgatcagg cggtcgtagt tttcgttgcg gttcagctcg ccaacagcgg ctccaagctg
      901 agtgctgttg tgccgctgcg acccgtcaat ggcatcgcgc aaggcgaagc cacgacgcgc
      961 cggaacttgc gcaagcttgt ccgagaagga aaatacagaa accttctccc cgatctcgcg
     1021 caacagcaca gccaacccgt acgccgcgtc cgtacgctgc atctccgaac ggcgggaaag
     1081 cggcgcggtc atcgagccag aaacgtccac cagcacaatg gtttttccgc tcaacttttc
     1141 cgcgttcgcc accgacttca gcattgcctg ctcgagcctc tcttcccact gcggagcgta
     1201 tcgagccgcc gccagaaaac gaaacggcag tactcgcgca gtgttcattt cgccgagcgc
     1261 ggcaagcacg agagactcgt ccacctgggc ctcgcgcata ttgcgaaggt tccgtagcaa
     1321 cgcaagagct ccaagcttct gctcgcgcag caatcgctcc cacgcttcgc gcttgtcgga
     1381 tccagcagac aacgccactt cccacgtatc aggcgttgcc agctttcccc agatcagctt
     1441 cttccacacc gatgcctgct caacatcgcg tggcttggcg tggctgagga agagcacatc
     1501 gcgcagcttg atcggcccgc ttcggtcgta cttggcaagc tggtattcgt cgaacttcgg
     1561 gaatgcagcc gccaatccct ttttcacctg tccggaaaga ggaacacgcc cgtccttcca
     1621 atagatggcc acgaattccg ccagttcgtc ggcacgctga atcacgcgcg ccaatgtctc
     1681 agacacgagc gcacgatggg tcttgtgccg cgccatttca cgagcaagca gcaacggagc
     1741 atggcgcagc ttcatctgct cacgagcttc cacggcaagc gccgccacct tttcagcggc
     1801 cactcgtggt acgagctcag caatgcggcc cgcaatctcg actccgtcct cataaaactg
     1861 cgattcccag agcaggcatg cgagcaccga tcgccgcaat tgcagttcga ccggaatgtt
     1921 acgcgcgggc gctccttcat gcgtgcgagg accaaaaata ttcagcgtgt tgagtcgagc
     1981 catagtgacc tccttcgttc aattgttagt ctcggatatt aactgtcagc aaaaacaatc
     2041 attcagcgcg attgtcattc tgagcgtagc aatactttgc gaaagcaaag tatcgcgaag
     2101 ttgaagaatc cctaacgtca tatccgcttt ttcgcgggat ccgtcgccaa cacattgcta
     2161 gatcaaaacg actaaccgcc caaaatacaa cttgaactac tgcaagaaaa acgccgggga
     2221 accagcgaca acggtatttg gtttccgatg ctctaccact gagctacacg gccaaagccg
     2281 cggcaggagt cgaacctgcg accttcggat taatcgaagg aaccgtagcc ttcaccaccg
     2341 gcgaacccta aaccagaatt ccgtgactct catctgtcgc cacggattcc gggtcttttg
     2401 cttcttgctc cagcgccaat agcagcccct ggaactccgc cattgtctgc gtggattcgt
     2461 gagcatccac aaattcacgg gcttcagcat ccgttgcctc cggaaaaaat ctgcgataca
     2521 gagaaatttt ttgcgaactc gcggctttcc ccagaaacag cctgtaatca atacgcccgg
     2581 gtctcaaaaa agcgagatcg agcgtttcga ttttgttcgt ggtcatcacg aacaaaatgt
     2641 cttccggtgc attgaagcca tcgagcacat tcaataaacc cgaaagcgtt accccgagca
     2701 ggctcgaaat ctcctctccc cggccgggtc cgttcggctg cttcaagttt tgcgaaggcc
     2761 tcgcgtctcc tgctttcatg cagtcaatgt cttcgaacaa aatcaccgag gtgcgggcac
     2821 gtcactgacc gcgcttttta acgttctgtc attaaactcc gtcaagttaa tcgcatacac
     2881 cgacatgcca aatgttgccg cgaatcccga caccaaagaa gttttacccg tccccggagg
     2941 cccatatagc agatagccgc gatgatatgg aactcccaat tcgcaatagc gcttccttgc
     3001 tgcccgaaac ctgcgaatgt cttcgagcag gtgttccttt tctccgggct tcggaatcac
     3061 cgcttccaac attcgcggcg tgtaaccgag cacccggatc cagccatcgt catatacgta
     3121 caaatacgag gcgaggcgga ttttcttctt gtggcaggca acgatctcgt ccacaaacct
     3181 cttcagaaac cattgttcgc gtcccacggt gcggaacgta agcgactcca agcggcgcgg
     3241 tgtccacccc ttggtctcct ctgttcgcga aaaccatacc cagaacggtc gtctttcata
     3301 ccaaaagcgg tgacggcccg gcgccggtat tagggccgat ccctcgccac gcagcgtggt
     3361 gtccaggtcc aggcggcgaa tgcgtttcag gaattcctgc tccagaaacc attcttttac
     3421 ccacacaaaa gccgtgtcgt cattccttca cagtcagtgt caacgtgctc tgctcaatga
     3481 tccattgcca taacttcgtt ggcagtgcgc gcaaataaac gctgataccc ccgatgatca
     3541 tcaacagcaa accgccagag gcaaactggt tctgaccgtt caaaacgtat tttaaaaatt
     3601 ccttcatgtt cttacatttc taaccggcct cggccggaag tttaaaaaaa cgaaaattat
     3661 ttgccgggga actcgctaga agagccgact cgaggcgcga tctctccggg taccttgtgc
     3721 atcgggacta cgcccccgtc cccggagctc tgatcctcgg caggaccgcc gtcctgcgcg
     3781 tcgttgtatg agcgaagtat ctctcctatt caccaccggc aaaatccaag ccgccgcatt
     3841 cgcggcgtcg catcaccccc ttgaacatta aaggtcaggg aacatgcggt cacggtaggg
     3901 cttgcgccca cgaggggttc gaaccctccc agcccgaagg ctgtttcgcc tgagaagtat
     3961 ccgtggcctt tcaccactga ccatgccatc cctcgcaaag cgcgggatgt aataacgtcg
     4021 tatcctcggc gcaatggaag cgcccagaat ttcgctagcg ggcacgcggc ccggggtgat
     4081 tcgcgcgggg cgcacgtctg cggaagctgt agctggggga ataaacggcg gcggtatagc
     4141 gatccacagt cgaaggaacc gcagcctgcg ccacccagcg atttagtttt ctagagtttg
     4201 cgttaaagag cgtcgaagat taactgacgc gatcggacga cgcccaatag caaccccggc
     4261 gcatgattgg ccgaagcagc ccgctaaacg ttttggttat gtgttgcgcc aaattgggca
     4321 tcctttcgta tgaagatgtt agaagatgcc acagcttaca attttttgca agagcaaaaa
     4381 atcgtggtag tagcgcagtt tgccaaatac gccaccccgg aaaaccaggt catctcgacg
     4441 ttgagcgaag cgaagaccgg agagacctta cgtgcgttga aagccatcaa tacagacaac
     4501 aaagatcgca gttgctgtca acactgacat gcactaccaa aattgcgctc gcgtaacgtt
     4561 tgtgtcccag tgagaaacgg cttatgtggg atgggacgcc tcctccatcc ggcggagcac
     4621 cgttcgagtt tttgaccaag tattaaggca acccccccga gtgcacccct tgcataacta
     4681 aactaaaccg tttagtattc ataaatccca aaaacagcgg agcttattcc atgagcgcca
     4741 tccgggttga aaacttagaa aaaaccttcg gcgatctgac cgccgtaaat aagatttctt
     4801 ttcaggtcga gaaaggtgaa atctttgcct tccttggccc caacggagca ggaaagacca
     4861 ccacaatcaa aatgttgacc acgctgctga accccaccag cggcaaaatc gaactggatg
     4921 gcctggatcc caccatccat cgcaatgagg ctcgccagcg cttcggaatc gtctttcagg
     4981 atcccagtct cgacgccgaa cagaccgctt acgaaaacat ggagctgcac gggatcctgt
     5041 acaaagtccc gcacaaactc cgcgtccagc gcaccgaaga attgctgaag ctcttcgagc
     5101 tttgggatcg ccgcgacgac tacgtcaaga ctttttccgg aggcatgaag cgccgcctgg
     5161 agatcgcccg cggctttctg catactccaa aaattctttt tctcgatgag ccgacgctgg
     5221 gcctcgatcc acaaactcgc aatcaactct ggacccacgt gaagagcgtg aacgaaagcg
     5281 aaaacacaac cgtgttcctc accacccatt acatggatga agccgaccgc gtggcgcacc
     5341 gcattgccat cattgaccac ggcgcaatcg tggccatggg cactccgcag gagctaaagc
     5401 agcaaaccaa cagcgaatcg ctggaacagg catttctggc cttgaccggc tccacgattc
     5461 gcgatgaaag cgcgacctcg caagatcaat tgcggcgttt cgcgcagatg tggaggaaat
     5521 gacgatgagg aaaacgaagg ggaggatgga aagatgagtg caatctatat tctctggctg
     5581 cgcgaactga agcggtacat tcgctcacgc gcgcagattg cggcttcgct tggccagcca
     5641 ttgctctacc tgcttgcgct tggctttggg ctcgggcctg tttttcaaaa ggcagggcag
     5701 ggcagctact ttcaatttgt agcgcccggg gttgtcagca tgacgattct ttt
//
LOCUS       DATNDT010000267         6058 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1303158, whole genome shotgun sequence.
ACCESSION   DATNDT010000267 DATNDT010000000
VERSION     DATNDT010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6058
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1303158"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="VN788_08600"
     CDS             <1..858
                     /locus_tag="VN788_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20626.1"
                     /translation="PPMIPLDVTLDTPAAEKKYHFEVIESPQLTPTLVAAAAYNGIIG
                     SPAYGEGSTLQLSGTIDVKGHTSVRLDDLFAPADQTTPAPFYLATQLQTDFGRIYSNP
                     YESPQIGRIELHVKTLPERRWATIDSAWLDRSVVSPGDTVKVKVLLRPYRGAAFVQDI
                     PVTIPPQTASGKLELVISDAEFLNRNVQSLTAGTQGQLPGLEEMIQLINRERHNDRLY
                     ATLLQPTPTVLVEDKEMPNIPASAINVLNQRQNPGGARLLWQSTAGEWSVQMHQVIAG
                     QDMLTITVK"
     gene            889..3192
                     /locus_tag="VN788_08605"
     CDS             889..3192
                     /locus_tag="VN788_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20627.1"
                     /translation="MTTWRMNLVCLLAVASVLAGTARAEHTRYWRQADFSDFHKGTAT
                     GVAIRSDGKIGPAPKFGPFSDPNLAYVWSLRLDSKARLYAAGGSDAKVLRFDEAGKPT
                     TVFESSELAAQSIAFDSSDNLYVGTSPDGKVYKVTPDGTKSTFFEPKTKYIWALAIDR
                     QNALFVATGDTGKVFVVAPDGKGRLFWQSRERHARSLAFDAKGNLLIGTEPNGLIVRV
                     GVTRKASADLPVAGTSFVLYETNKAEVTSLALDSSGNIYAASIGDKGHLPELPRVLPT
                     PAPQVVSPVISSQATVITPGQRQPSPQPAPYRPFPTVSATGGAEVVRIAPDGSPETLW
                     TSHDSLVFALGFSLTGKLLLGTGDMGSLIELEGNDVYSSVATTASAQVTSLITGGGGK
                     VFVATANPGKVFTLGPGYEPNGTFESSTFDARIFSHWGRATWWGQGGSAKAKVEFFVR
                     SGNTSSPAEDWSPWAGPYKDSGTQAVDCPAARFVQWKAVFSDVTEESAPTISWVSLAY
                     QPKNVAPVIDDIAVQDPGIRISGFPSPTSGPGSPMPVALRIPQGPGGTPSAAPMDAEA
                     RPSESNSEPMPQGFTQKGYQSVLWSAHDDNDDHLLFTIYYRGEGDNDWRLLKDKITQR
                     YYSWDTTSMPDGAYYLKIVASDSPSNPAAEALWTQRESDRWEVANTPPRIENLRAGSG
                     ILNTKASFEAVSSSGPIARALYSIDSGDWKIVFPTGELSDAPKESYFMQLPGLPVGEH
                     TLAVQVTDRLNNTATAKTTFTVEPHSQ"
     gene            complement(3242..3622)
                     /locus_tag="VN788_08610"
     CDS             complement(3242..3622)
                     /locus_tag="VN788_08610"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU20628.1"
                     /translation="MSGAERRISPRKPCVVPVRFRIVANGGRETLGLREAARSSMTHE
                     GEVLNLSENGVYFTSRAKLSVGEPLEMHLTLPRELTGRNPEQVRCSARVVHVEDLSDS
                     GGTAGVGAVVDRFETVAASRDWSN"
     gene            complement(3859..4161)
                     /locus_tag="VN788_08615"
     CDS             complement(3859..4161)
                     /locus_tag="VN788_08615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20629.1"
                     /translation="MTLRVRASIHVALQGNRETIDVATLSVNPHGAMVVMDRNLAADT
                     RLVLEHGATKERVACKVVRSSQKMPEGFHVPLEFDSPAPGFWKIDFPSPDWRPEET"
     gene            complement(4303..4578)
                     /locus_tag="VN788_08620"
     CDS             complement(4303..4578)
                     /locus_tag="VN788_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20630.1"
                     /translation="MNSRVPVTIEWNGAAGPAHFEPAFTRVVNPYGCLLASPTEIRMR
                     ENLRITNLVTKQIANGLVVWKGAQRPDGWDIGVELIAADLNFWGVDL"
     gene            complement(4822..>6058)
                     /locus_tag="VN788_08625"
     CDS             complement(4822..>6058)
                     /locus_tag="VN788_08625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXU20631.1"
                     /translation="VMQEMRMKLGRIPGILVFLRNPPPIQIGGDVTESPYQLTLQSPD
                     TEELYRVATNFEQKMSGIPTITDVTSDLQIASPQVDVKIDRDKASALGVTASQIEDAL
                     YTAYGQRQISTIFAPNDEFWVVMELEDRYQKDPTALSLLYIRSSGGNLVPLSAVASLG
                     TSLGPLTVNHLGQLPSVTISFNLKPGTAIGEAVNQIDDLTRSLPASVSSQFQGTAQAF
                     QSSLVGLGILLVIAIVVIYIVLGILYESFIHPITILSGLPSAAFGALLTLQIFGYSLD
                     LYGFVGVIMLIGIVKKNAIMMVDFAIAREREGHLSASEAVYQGCLVRFRPIMMTTMAA
                     LMGTLPIAVGMGAGADSRRALGLAVCGGLIFSQLVTLYLTPVFYTYMDTFQSWMERRF
                     GRIAGIAKPRVTEEVFSAD"
BASE COUNT         1320 a         1871 c         1657 g         1210 t
ORIGIN      
        1 ccgcccatga ttccgctcga cgttactctc gacacgcctg ctgccgaaaa gaaatatcac
       61 tttgaagtga tcgaaagccc gcagttgacg cccaccctgg tggccgcggc agcgtacaac
      121 ggaatcatcg gcagccccgc atatggcgaa ggctcgaccc tgcaattgag cggaaccatc
      181 gacgtaaagg gacacacatc ggtgcgactg gacgacctct ttgcgcccgc cgatcaaacc
      241 actccagctc ctttttacct tgcgacacag cttcaaaccg acttcggacg catctactcg
      301 aatccctatg aatcacctca aatcggccga atcgagttgc acgtaaaaac gctgccggaa
      361 aggcgctggg ccacgattga tagcgcctgg ctggaccgaa gtgttgtgag tcccggcgat
      421 acggtaaagg tgaaagttct gctgcgaccc taccggggcg ccgcctttgt gcaagacatt
      481 ccggtgacca ttccaccgca gacggcaagc ggaaagctgg agctggtcat cagcgacgcg
      541 gaatttctga accgaaacgt tcagtccctg acggccggca cccaaggtca gctgccaggg
      601 ttggaagaga tgatccaact catcaaccgc gagcggcaca acgaccgcct ctacgccacc
      661 ttgttacagc cgacgcccac cgtgctcgta gaagacaagg aaatgcccaa catcccagcc
      721 tcggcgatca acgtactcaa tcagcgccag aatcctggcg gcgccagact tctatggcaa
      781 tccacggcgg gcgaatggtc agtgcaaatg caccaggtga tcgccggcca ggacatgctg
      841 accatcacgg tgaaataacg cgcacccttc caacggagat aaaagctgat gacgacttgg
      901 cgaatgaatc ttgtgtgcct gctggccgtc gcctccgtgc ttgcgggcac agcgcgtgct
      961 gagcatacgc gctattggcg ccaggccgac ttctccgatt ttcataaggg caccgcgacg
     1021 ggagtggcca ttcgcagcga cgggaaaatt ggccccgctc ctaagttcgg cccgttctct
     1081 gatcccaatc tcgcctacgt ctggtcgctg cgcttggatt cgaaagcgcg actctacgcg
     1141 gccggcggat cggatgcaaa agttcttcgt ttcgacgaag ccggaaaacc cactaccgtt
     1201 ttcgaatcca gcgaactcgc agcccagtcg attgcgttcg atagctcgga caatctctat
     1261 gtaggaacct cgccggacgg caaagtgtat aaagttactc ccgacgggac caaaagcacg
     1321 ttcttcgagc ccaaaaccaa atatatctgg gcattggcca ttgaccgcca aaacgctctg
     1381 ttcgtggcca cgggcgatac cgggaaagtg ttcgtcgtag ctcctgacgg gaagggccgg
     1441 cttttttggc agagtcgcga gcgccacgcg cgatcgctgg cattcgacgc gaagggcaat
     1501 ttattgatag gcaccgaacc gaatggtctt atcgtgcgcg tgggtgttac gcgcaaggcc
     1561 tccgctgacc ttcccgtggc tgggacgtcg ttcgtgcttt acgagacaaa caaagccgaa
     1621 gtaacttctc tcgccctgga tagcagcggc aacatctacg cagcgtccat cggggacaag
     1681 ggacacttgc cggaactgcc gcgagtcctt ccgaccccgg cgccccaggt cgtctcaccg
     1741 gtgatctctt cgcaggcgac agtaattacc cctggacaaa ggcagccgtc gccgcagccc
     1801 gccccctacc gcccctttcc gaccgtttcg gccactggcg gcgcggaggt cgtgcggatc
     1861 gcacctgatg gttctccgga aacgctttgg acatcgcacg atagccttgt ctttgccctc
     1921 ggattttctc tcaccggaaa actcctgctt gggacgggag atatggggag tctgattgaa
     1981 ctcgaaggta acgacgtcta ttcaagcgtc gcaaccactg cttccgcgca agtgaccagc
     2041 ttgatcacgg gcggaggcgg caaagtcttt gtcgctacgg ccaacccggg gaaagttttc
     2101 acgctcggac cgggctatga gccgaatggc acttttgaat cgagcacctt tgacgctcga
     2161 atattctccc actggggacg ggcgacctgg tggggtcaag gcggctccgc aaaagcaaaa
     2221 gttgagtttt tcgttcgctc cggcaacacg tccagcccag cggaagattg gagcccctgg
     2281 gccgggccat acaaggactc tggaactcaa gctgtggact gtccggccgc ccgcttcgtg
     2341 cagtggaagg ctgtattttc tgacgtcacc gaggaaagcg cgcccacgat ttcctgggtc
     2401 agtctcgcct accaacccaa gaatgtcgcg cccgtaattg acgatatcgc ggttcaggac
     2461 cccggcattc gaatctccgg attcccctcg cccacaagcg gaccaggcag tcccatgccc
     2521 gtcgccctga gaatacccca gggtcctggc ggaacccctt ccgcggctcc tatggacgcg
     2581 gaagcccgtc catctgaatc aaacagcgag cccatgcccc aaggtttcac tcagaagggc
     2641 taccagagcg tgctgtggag cgcgcatgac gacaatgacg accacctgct cttcacgatc
     2701 tactaccgcg gcgaaggcga caacgactgg cgtctcctga aggacaagat aacccagcgg
     2761 tactactcct gggataccac ttcgatgccc gatggagcct actacttgaa gatcgtcgcc
     2821 tcggattcgc cttcgaatcc cgcagccgaa gccctatgga cccagcgcga aagcgaccgg
     2881 tgggaggtag ccaacacacc gccccgcatt gagaatttgc gcgctggctc gggaattctg
     2941 aataccaagg cttcgttcga ggccgtcagt tcctcgggtc caatcgcgcg cgccctatac
     3001 agtatcgatt cgggtgattg gaagattgtt ttccccacgg gcgagctctc ggatgccccg
     3061 aaagaaagct actttatgca attacctggg cttccggtgg gcgaacacac gctggcggtt
     3121 caggtaacgg accggcttaa caatacagcc accgccaaga ctacattcac cgtcgagccg
     3181 cactctcaat agcttgccgg gcttctcacg cgaatcatcg tgcgggcacc gaggtctccc
     3241 gctaattgga ccaatcgcgc gatgccgcaa cggtttcaaa gcggtcgaca actgccccca
     3301 cgcctgcggt ccctcccgaa tcggaaagat cttccacgtg gaccactcgc gcgctgcacc
     3361 gtacttgttc gggatttcgt ccggtgagtt ctcgcggaag cgtcagatgc atttcgagag
     3421 gctcacccac gctcagtttc gcccgcgagg taaaatacac gccgttttcc gataaattca
     3481 gcacctcgcc ctcatgcgtc atggaactgc gagccgcttc gcgcaagccc aaagtttcgc
     3541 ggcccccgtt cgctacaatg cgaaaccgca caggcaccac gcacggcttt cggggcgaga
     3601 tgcggcgttc tgcacctgac atgacgtcct cctgacgagc aagtagccat cttttcgcaa
     3661 ctgccggaag ggatgatgcg aggatgtcag cgggtgcgga ccccgtcaat aattttaatg
     3721 tgcgaaagtc gcgtattgtg tagcgtagcg cggacgcgag gaaccggcgc taatacaacg
     3781 catcgccaag gtgagcgatg cactggccga acccttgaag cctgcccatt gaattattag
     3841 cgcgcactaa cctgactgct aggtctcctc agggcgccag tcgggggatg gaaaatcgat
     3901 tttccagaaa ccgggcgccg gggagtcaaa ttccaacggt acgtgaaacc cctccggcat
     3961 tttctgggaa gatcgcacca ctttgcacgc tacgcgctct ttggtggcgc catgttccag
     4021 caccagtctc gtatcggctg ccaggttgcg gtccatcact accatagcgc cgtgcggatt
     4081 cacgctcagc gtggctacat cgatcgtttc acgatttccc tgcaaggcca cgtgaatgct
     4141 ggcgcggacc cgcagtgtca cccgctggcc gcgtcggcga tccgaacccc ctggcgctgg
     4201 cgccaaaggt gcaattctaa ggattggctc aggcagggcg tatgctccgc tctgaactgg
     4261 ctgattcggt ggtttcggag tcatttttca gggcgttggt cctcaaagat caactcccca
     4321 gaagttaaga tcggccgcaa tcaattccac tccaatatcc cagccatccg gccgctgggc
     4381 tcccttccaa accaccaaac cgttggcaat ttgctttgtc acaaggttgg taatccgcaa
     4441 gttttcgcgc atccggattt ccgtcgggct ggcgagcagg cagccgtagg ggttgaccac
     4501 gcgcgtaaac gccggttcaa agtgcgccgg ccccgcagcg ccgttccatt caatcgttac
     4561 gggaacacgg gagttcattc gcggcccgcg ccgattgctc ctcattcgta tgtcgcgtgg
     4621 aagctcttcg ccgaaaccca ttctggtcga atctccgcgg aatggcgaac cggaatccag
     4681 ccgtcctaac gccgtgccgt tcaccagtgc cagaatgatc ctaaacgggt ggaagatttt
     4741 cggtcaatgg taccagcaga cagggggttt actctacggt tagcgagcag actgtcgaag
     4801 ccgctgagaa attgtccaaa tctaatctgc ggagaaaact tcttcggtca cccgaggctt
     4861 ggcgatcccg gcaatccggc caaaccgccg ctccatccag ctctggaacg tatccatgta
     4921 tgtgtaaaac accggagtga ggtatagggt gaccagttgc gaaaagatca gcccgccgca
     4981 aaccgcgagc cccagcgccc ggcgcgaatc cgctccagca cccatgccca cggcaatcgg
     5041 caacgtcccc atcaacgcag ccatggtcgt catcataatc ggccggaaac gcactaggca
     5101 gccctggtaa acggcttcgg aggcgctcaa gtgcccttcc ctttcgcgcg caatcgcaaa
     5161 atccaccatc atgatcgcat tcttcttcac gatcccaatc agcataatca cgcccacgaa
     5221 tccgtaaagg tcaaggctat acccaaaaat ctgcagcgtc agcagcgcgc caaacgccgc
     5281 cgatggcaga cccgatagaa tggtgatcgg atgaatgaag ctttcgtaga ggatgccgag
     5341 cacgatgtag atcaccacga tcgcgatcac cagcaggatg cccaggccca ctaatgacga
     5401 ttgaaatgcc tgggccgtcc cctggaattg cgacgatacg ctcgccggga gcgatcgcgt
     5461 cagatcgtca atctggttca ccgcttcacc gatggccgtc ccgggcttca ggttgaatga
     5521 gatggtcacc gagggaagct ggcccaagtg gttcactgtc aacggcccga gactagttcc
     5581 caggctcgca acggcgctta gcggaacaag gttgcctcca ctcgaccgta tataaagcag
     5641 cgaaagcgcg gtggggtctt tctgatacct atcttcgagc tccatcacca cccagaattc
     5701 gtcgttcgga gcgaaaatcg tcgaaatttg acgctggccg taggccgtat acagcgcgtc
     5761 ctcgatctgt gacgcagtca cgccaagcgc ggaagcttta tcacggtcga ttttgacgtc
     5821 cacctgcgga ctcgcaatct gcagatcgct ggtgacgtcc gtgatcgtcg ggatgccgct
     5881 cattttctgc tcgaagttcg ttgctacgcg atacagttcc tcggtatcgg ggctttgtag
     5941 ggtcagctga tagggacttt cggtaacgtc cccgccgatc tgaatcggag gcgggtttcg
     6001 cagaaacaca agaatgccgg ggattcgtcc aagcttcatc cgcatttctt gcatcact
//
LOCUS       DATNDT010000268        18290 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3495565, whole genome shotgun sequence.
ACCESSION   DATNDT010000268 DATNDT010000000
VERSION     DATNDT010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 18290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18290
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3495565"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="VN788_08630"
     CDS             complement(<1..993)
                     /locus_tag="VN788_08630"
                     /inference="COORDINATES: protein motif:HMM:NF037284.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20632.1"
                     /translation="MAEATVIQGPAAAGTQKLCLEIVRFPNGNEAKLVRAKPDDIIDD
                     LFGVIGISKQRSLILLLGGADRLDDALLPKLRQVFNRGIARAALRIGALIIDGGTDAG
                     VMALMGRAIADNGRQTPLLGVAPAAKVTYPGGPADADSGGSAPLNPNHSQFVLTPGSE
                     WGDETDTLFRLARNLAGTNPVLAILVNGGDTAQAEVLRCVRLKWPVLVVQGSGRLADT
                     IAGLIASPGTRADDADLDEIRCDGRIRVVRLDDDLSLIERVIEGRPAQDPVLVLALRR
                     FHAYDQTAELNQNRYRRSQGVILLLGVLATLLAIYHQHEPAAGDVVGHVLQIAII"
     gene            complement(1056..2174)
                     /locus_tag="VN788_08635"
     CDS             complement(1056..2174)
                     /locus_tag="VN788_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HXU20633.1"
                     /translation="MKITRVETIYLRLPEVKEQCDSGQDALIVRVETDAGITGIGEVD
                     SSPMAVKGAIDGPFSHTTTSGLAHLVLGEDPFETEKLWHKMYRGNIYAGRRGIGIHAM
                     SGIDMALWDIKGKAFGMPVWKLLGGGFHRKIRCYASSLFGPTPKETGERARAYADRGF
                     TAVKFGWDPMGRDEKTDIALVCEARKGLGDKNDLLIDAGLVWDAKTAIQRAHAFADSN
                     IYWLEEPLRPDDYEGYRKLSKATRIRIAAGEEESNRQSYLDLMDRGHIDVVQVDLTRC
                     GGFTEGMKIASLAADRGLPVANHGFTTYINVAAALHWLNSIPNALICEFVAESGTTLR
                     DSITRQRIVAKDGYLEIPQEPGLGIELNDDAVSKYRVA"
     gene            2255..3013
                     /locus_tag="VN788_08640"
     CDS             2255..3013
                     /locus_tag="VN788_08640"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU20634.1"
                     /translation="METRRVALITGAAAGIGAGIALRFAEAGYAVAAFDIDGAGAARM
                     AEQLRGLAPSVAIGGDVSREDDAQAAAEKAVSEFGSLDVLVNNAGIEVNGSVSDLTSE
                     AWDRQIAVNLRGVFLMSKYAIAKMRGRGGAIVNISSVHAFVSYASCAAYDTTKAGLLG
                     LTRAMALDHGVEGIRVNAICPGYIDTPLMDRWLDSAPDREETLRQVLSYHPLGRMGTP
                     RDIADATLFLASDAAAFISGASLVVDGAMSVAGH"
     gene            complement(3123..4061)
                     /locus_tag="VN788_08645"
     CDS             complement(3123..4061)
                     /locus_tag="VN788_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20635.1"
                     /translation="MGNGKKDTPNPSSTSIPDLQREIGTLIERSKWHRTIGWAAISAA
                     TIVILFLFGWIFLHLPVEAKHDSDAVNERISAINERLATLTTTIEFMKPDPGKRIPTI
                     MNDNLHSGENLDLGLKTIGAVARRATKEKIHSNEASISEVGQRIVRTALKQDANAWAV
                     ASQVLDYVSFLRSSRLSSLGYAIPLSDQIGHFDIGEAIEQKDPITFSFINFANVASAA
                     IAEHIGGGPTYTSDQKEGPALLIITSGPRTILKLDQHRLRNIVFVGMGIEYDGGPADF
                     KKVTFINCNFKMPSTSGSAHFALAVLSAPVTNFTKT"
     gene            4358..7597
                     /locus_tag="VN788_08650"
     CDS             4358..7597
                     /locus_tag="VN788_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20636.1"
                     /translation="MRIRVALSLLILPVACACHLRAAKLLDIAPSGQLTTWTDAGLRH
                     LANVHAAVGASNLGVEWDEERDVREIRVRYNGTSAKGAAVEYWFKYWPYDPPKMPTIE
                     DPMDDPWQGKWLKAETTEICDGAECRYDFKPLAETENPRADKLPGVVYRRTLKARLIY
                     AGGIPDIASLDVFTGTEQKPVNLRVEFSPGEITAHRTSTASVYNGILRSVKPWQVSKG
                     LLLDLIAAKPNLPGSHDLTVVTIHATPTDTFSFRVDDLKHGPISIPQFHARIVDITPG
                     APQAPAAPGKLRVRERIPREPEQTYQRASREIPPLDPWNREGGGRVYLPLAPDSDWQK
                     FAFEYGGNVFISKRGIKAKGRELARLQWTGDTITWKIGTGATPYYRDDRKVAVRVLDG
                     YLPVVTQRWENEGLRYDQEAYATLLRGPLSPTGSGRDEETPAILMMRLAATNSTRSAR
                     KAEIWLSVDGGGILTVSGKSVYGNGKLIAELGENPGAVETKAHSVHISFDVPPSGSNA
                     VVFKLPAVSDLNASDIEDLNRLHYSSERQQLITYWKSVIDPAVRFRVPEPKFNALARA
                     VIAHIHISTTKDPKTGLYMVPAASYSYDEFENEACYQVLLLDTLGQFDTAASYLEPMM
                     RLQGSQDFPGAHKGPQDAIFHGTKIDDVYNYTEQGYGLDHGTVLWTLAQHYLYTRDKT
                     WLEHAWPHMKKAIDWIVEQRSTTKLTDIHGQKIRAYGLLPASQLEDNADWANWFPVNA
                     YAWAGMFLTSEALADIGNPEAANIRKKADEFRADLRSAILRSVEAAPVVRMQDGTYEP
                     YVPVLPTRRFRLFGPIRMDYYQRYPGPRIKPLLRLGADRDTLCGAVVLLILNVFSSNE
                     PITNWILNDWEDNETLSSGMGMNIHGMTDDRYWFSQGGMVFQANLVNPIQVYLRLHEA
                     QAAIRNLYNDFVTCLYPEVNQFTEEFHQWRHGSGPFYKISDESRFVNRVRDTLVFEDQ
                     DSLWLAAGTPRRWLDSKEGIHVNRVQTFFGPVSYDIHRGRRPGMLEATVELPSSRPPK
                     HAWLVARVPSGHIQSVTVNGKPWSKINRRLEAIELPQQQGFLKLAIRYQ"
     gene            complement(7581..8162)
                     /locus_tag="VN788_08655"
     CDS             complement(7581..8162)
                     /locus_tag="VN788_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20637.1"
                     /translation="MDSSSTYANARRDEVRVFINRIAALAACLHLIASLSIADGGAVL
                     SRVHTGSLIVTVFSTSVPLRVGAADLSVMVQTAASGSPELDADIDLRLSKPGGPEIRI
                     AATRTRSANKLLYTAQPILPSPGEWHLDVHVQANGNTIAVPVSISVLTADPPLVTYWP
                     YFLAVPLGMLLFILNQWLKSRMQPRRRAVTGIV"
     gene            complement(8117..10273)
                     /locus_tag="VN788_08660"
     CDS             complement(8117..10273)
                     /locus_tag="VN788_08660"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HXU20638.1"
                     /translation="MMDTIVSAALSSWRLDPRVVAILLGAGVVYVLGWLRGRRLIRGE
                     KDLTRLAAFLCGLAILFLALESPLDAFDSLFLSAHMTQHMLLMMIAPPLLLLGHPLVP
                     LLRGLPRKFVKEGLVPFLSWPLLRTAWRWLISPPVAWLTLAVSTIVWHLPKFYQLALR
                     SPGWHSAQHACFFWTGVFFWWPVIESGHGGSRWPRWAMIPYLLLADVINTALSAFFIF
                     SGRVLYPVYETARVGGISGLHDQATAGAIMWVPGSLVYLLPAIVIAVDQFSGTAMVKS
                     RELIAERIPIRRPRTTTKSRLPKLRRAAQSAMLLLAIAVMADGFRGTQVAPMNLAGAL
                     PWIHWRALSLLALLLVGNLFCMACPFTLARDLGRLVFPARLRWPRLLRTKWLPAALFV
                     FYLWAYEALSLWDSPWLTAWIVAGYFATILVVDGVFRGASFCKYVCPIGQFHFVSSLI
                     SPGEIGIRSAQVCHSCRTHDCIRGNDRDHGCELDLFQPKKIGNLDCTFCLDCVKACPH
                     DNVALKAVVPASTLTLDPDRSSIGKLSKRTDIAVFAIIMIFGAFVNAAGMVMPVMAWE
                     HSWHARLGAGAMPLVIAALIVGGVVILPGAVVCLCGVLNRIMDASIPIADVMRRFALA
                     LVPLGVAMWAAHLLYHFATAWNASIIWLTPAQLLLLDTGLLLTLYVLWRLARQYSKYV
                     GRAIGLITPWALVSCGLYGLGVWILLQPMQMRGVMK"
     gene            complement(10274..11737)
                     /locus_tag="VN788_08665"
     CDS             complement(10274..11737)
                     /locus_tag="VN788_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXU20639.1"
                     /translation="MKWLRKTAVWLDSRLHITALFASTAGHSVPASASSWFYVFGSAT
                     LLCFVIQVITGACLAFVYVPSTNEAWTSLQYLNHAQYLGWYLRAVHNWGSNFMIAIMT
                     LHMIQVFLFGAYKYPRELTWISGVFLLLLTLGMAFTGQILRFDQDAYWGLGIGASITG
                     RVPLIGAELVRVLLAGPIIAGETLSRFFTLHVFIIPGLIIAVVSMHLRLVLTKGINEY
                     PKPGYRVNKSTYDAEYAELLREKGVPFVPNAVGKDLIFGAIVIVGILTCAAVFGPNGP
                     SGPPSPTIVDANPRPDFYFLSIFAALALLPDWMEVLVLFVAPAVLIGFLLALPFIWGD
                     GEKSARRRPVAVLSVIVIMLVLGVLTYMGKTSPWSPQMSAWTSNAIPPRYLNGRTPLQ
                     LHGAILLQNKQCRNCHSLGGKGGERGPALDEVATRLTRAQMIRQVIQGGGNMPAYGKS
                     LKPAEVEALVSFMLTLRPAAVPAVRDSAEPAQPKLNP"
     gene            complement(11752..12291)
                     /locus_tag="VN788_08670"
     CDS             complement(11752..12291)
                     /locus_tag="VN788_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXU20640.1"
                     /translation="MSDVSHGAGSENANTTPPTRRHVLFLAGAALDVVAAALLAVPLI
                     GFVFSSFANRRDPRKWISLGRLEYFPESQTRLATYQNPFTRPWDGETAHIPCWVRHIK
                     GNHFQVFAINCAHLGCPVRWFRESQLFMCPCHGGVYYEDGSRASGPPPRGLFEYDYKV
                     QNGELWVKGGQMPTLANPV"
     gene            complement(12310..12987)
                     /locus_tag="VN788_08675"
     CDS             complement(12310..12987)
                     /locus_tag="VN788_08675"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU20641.1"
                     /translation="MSAFTGLAICLFLMGVAGCGKPKPAQASKSEPDFHTLFNENCSG
                     CHGNDGKDGPGRPLNDPFYLALIPKDTLEDTIEHGRPGTAMPAFAESDGGPLDPNDIK
                     ALVSGIEREWAKPVSLGGATPPPYSATNDAGDPVAGKRLFTRDCFACHGTGAPIGPVT
                     DASYLMLVSDQWLRSSIIAGRHDRGMPDWRHLNMGRALSNQDVSDLVSYLVSLRPTGA
                     ANKRETQ"
     gene            complement(12984..13850)
                     /locus_tag="VN788_08680"
     CDS             complement(12984..13850)
                     /locus_tag="VN788_08680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20642.1"
                     /translation="MEYEGHGEAGQRTEQTHIEMPAPTFWPMVLAFGLALLLGGLVTN
                     FVVSLVGLVIAIRSAIGWWRDVIPLEKHEQVAIEQDRRPDPIMVEARSVIRLQVGEGQ
                     HRAHIPERIHPYSAGLWGGLAGGAVMAALACLYGLVAQHSIWYPINLLAGVVIPSISS
                     ASLEQLRAFNGLAFVAACVGHTVLSILVGVVYAVILPMFPKHAPIWAGILMPLFWSGL
                     IATALQMLDPALNERINWPWFVVCQLGFGLVGGFVIARSTSIHTMQSWGFAHRAFIDA
                     PGISPRPPEDRQ"
     gene            complement(13861..14466)
                     /locus_tag="VN788_08685"
     CDS             complement(13861..14466)
                     /locus_tag="VN788_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HXU20643.1"
                     /translation="MATSTLVPASPENLEREWKLPDRGMIGIGFLIITESSLFSIFIV
                     SYLYYIGKNLSGPYPKDVLDIPIVATVCLLSSSLTMIMAERALKRNQLGRFKLWWTVT
                     ILLGAEFLISTGVEWHHLIYIDHLTIATNLFGTTFYSLVGLHASHVIVGMTFLLLVLI
                     LTLSGFPIQTQERRVAFLSWYWHFVDAVWVVVFSVVYLIGR"
     gene            complement(14472..16151)
                     /gene="ctaD"
                     /locus_tag="VN788_08690"
     CDS             complement(14472..16151)
                     /gene="ctaD"
                     /locus_tag="VN788_08690"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HXU20644.1"
                     /translation="MAASDYPLEYPSHRHAEFSLSETLYECVTTVDHKRLGLMYIVAS
                     LIFMVIGGIMASVIRLQLAVPDAHVVSPETFNRLFTMHGTTMVFLLGMPILAGFANYL
                     VPLMIGARDMAFPRLNAFGFWAFLFGGLLLYSSYIGGGGLSGAGSAPDVGWFAYSPLT
                     ERAFSRGASTDFWILAIMLTGVGSISSAINVIATIASMRCPGMTLIKMPLFVWMMLVV
                     SWMIILALPPLSACQIMLLLDRFLGAHAFDTQAGGSAVLWQHYFWIFGHPEVYILVFP
                     AFAVMSEIIPVFSRKPIFGRQAMVGAAVAIGFISMSVWAHHMFAVGMTSTANMFFVAA
                     TMLIAVPTGIKIFNWLATMWGGKLRFATPMLFCCAFLFQFLVAGLTGVMLGVAPFDWQ
                     LHDSYFVVGHFHFVLIGGIIFALFGGMYYWFPKASGKMMSETLGKWHFWLFCVGFNMT
                     FIPMHFLGFLGMPRRIYTYAPGRGWDLLNMVASIGVIFQIAGIACFIWNVIQSLRSGE
                     PAGDDPWDGWTLEWTTSSPPPEYNFEKLPVVRSSRPLWDLKHPQDPDWKFE"
     gene            complement(16164..17171)
                     /gene="coxB"
                     /locus_tag="VN788_08695"
     CDS             complement(16164..17171)
                     /gene="coxB"
                     /locus_tag="VN788_08695"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HXU20645.1"
                     /translation="MLALAQAALLQTGVPALAEHRIANIFRPLATPADSEYKLALFVL
                     SITGAIFVIVGGLLLYAMIRFRRKPGDDARQEPPQVYGSNQIEVAWTVIPILIVFILL
                     GVTVRMIGAIQDAQPGPSALRATIVGHQWWWEIHYPDLGITTANEFHVPVADASGHAT
                     FMKLESMDVIHSFWVPQLSGKTDLIPNRDNALWFDPREPGVYLGNCAEYCGQQHANML
                     LRVIVHREGDFEKWAAAQSKPAEDNPAVSQGKAMFESLSCVSCHTVRGTSAKGDVGPD
                     LTHLMSRETIGSGVLPNTPKNLRDWVKDPQAIKPGCLMPSMRLNGQELDQVVAYLESL
                     K"
     gene            17290..>18290
                     /locus_tag="VN788_08700"
     CDS             17290..>18290
                     /locus_tag="VN788_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20646.1"
                     /translation="MENAARKSNLSRLLPLRIAAVLAIAVNFFWFTAGIFGSHFAPDE
                     MMNMGWAWEAGIAKILANLAQFYTTFYRPTGALYYWTLHRLFGLNPVPYRVIDLAVLA
                     FNVWLAYRLASVLSRSRSVAWLAAFLFSYHEKIMIWVAYNGAWVYDRLCFTFSISALL
                     VYVRARSRGGEISLRRGAAILVLYVLALGSKEMAVAVPVLLIAYECVYAFRTWRQNRN
                     AWLLITLLCVMTALYAYGKTHGPDSLINNPQYRPVLNAATYLRSQARHMQDYFFLNHA
                     FSPAIAAFILLAMAAAAFAFRSRPLIYAIAFLIFSPLPIAFINRSGGSLYIPLFGWCL
                     "
BASE COUNT         4251 a         5369 c         4858 g         3812 t
ORIGIN      
        1 gatgatcgca atttgcagca catgtcctac aacatctccc gctgcaggtt catgttggtg
       61 atagattgcc agcagtgtcg ccaggactcc gagaagcagg atgactccct gcgatcgccg
      121 gtatctgttc tgattcagct cggcggtttg gtcatacgcg tgaaaccttc gcaaagcgag
      181 caccagcacc gggtcttgcg ccggcctgcc ttcgatcacg cgctcgatca gagaaagatc
      241 gtcatccaag cggacgaccc gaattctacc gtcgcagcga atctcgtcga gatccgcgtc
      301 gtcggcgcgc gttcccggcg atgcaatcag ccccgcgatg gtatccgcca gccgcccgct
      361 tccttgaacc accagcaccg gccacttcag ccgcacgcat cggagtactt ccgcctgagc
      421 cgtgtccccg ccattcacga gaatcgccag taccggattc gttccggcca gattccgcgc
      481 gaggcgaaac agcgtgtcgg tctcatcgcc ccattcggat cccggcgtaa gaacgaactg
      541 gctgtggttc ggattcagcg gtgcggagcc accggaatcg gcatccgccg ggccccccgg
      601 ataagtcacc ttggcggcgg gcgccactcc gagcagcggg gtctggcgcc cgttatccgc
      661 gatggcccgc cccatcaatg ccatcacgcc ggcatctgtt ccgccatcga tgatcagagc
      721 gcctatgcgc aaagcggccc gagcgatacc ccgattgaaa acctggcgta atttcggcag
      781 caaagcgtca tccagacgat ccgccccgcc gagtaacagg atcaaagagc gctgtttgct
      841 aatgccgatc acgccgaata gatcgtcgat aatatcgtcc ggtttggcgc ggaccagttt
      901 cgcttcgttc ccgttcggga atcgcacgat ctccaggcag agcttttgcg ttccagcagc
      961 cgccgggcct tgaatcacag tcgcttcagc caacccgcac ctcctgagtc tctcaagtgt
     1021 accctagggt tttaatgggc ttagcagtat ccgagttagg cgacgcgata ttttgaaacc
     1081 gcatcgtcat tcagttcgat tcctaagccc ggctcttgcg gaatctccag atacccatct
     1141 ttcgccacaa tccgctggcg tgttatagaa tcgcgcaaag tcgtgccgct ctccgcgaca
     1201 aactcgcaga tcagcgcatt tggaatcgag ttgagccagt gaagcgcggc cgcgacgttt
     1261 atgtatgtcg tgaatccgtg attcgccacg ggaagcccgc ggtcagcagc caggctcgcg
     1321 atcttcatgc cttcggtgaa gccgccgcag cgtgtcaaat ccacctgcac gacatcgata
     1381 tggccgcgat ccatcaaatc taaataagac tgccggttgc tctcttcttc ccctgccgcg
     1441 atgcggattc gcgtggcctt gctcagcttg cgatagccct cgtagtcgtc cggacgaagc
     1501 ggttcctcca gccagtaaat attcgaatcg gcaaaggcgt gcgcgcgctg gatcgcggtt
     1561 tttgcatccc acaccaagcc cgcatcgatc aaaaggtcgt tcttgtctcc caagcccttc
     1621 cgagcctcgc acaccagggc aatgtcggtc ttttcatccc ggcccatcgg atcccaaccg
     1681 aacttgacag ccgtaaatcc gcgatctgcg tatgcccgcg cgcgttcgcc ggtctcctta
     1741 ggcgtcggtc cgaacagaga acttgcgtaa catcggatct tccgatggaa gccgcctccc
     1801 agaagtttcc agaccggcat tccgaaggcc ttccctttga tatcccacag cgccatgtcg
     1861 atcccgctca tggcgtgaat gccgattccg cggcggccgg cgtagatgtt tccgcgatac
     1921 atcttgtgcc agagcttttc ggtttcaaat ggatcttctc cgagcacaag atgcgcgagc
     1981 cccgacgtgg tcgtatgcga aaaaggcccg tctatcgcac ctttaaccgc catcgggctc
     2041 gaatccactt ctccgatccc ggtaatcccc gcgtcggtct caacgcggac gataagcgca
     2101 tcctggccgc tgtcgcattg ttccttcacc tcggggaggc gtaggtaaat cgtttccacg
     2161 cgcgtgatct tcatttgata atccaatcat agaggatgac tttcactctg cacgaattga
     2221 cgatttcctt gattcgtggg aactctaagg agttatggag actcgaagag tagcgctgat
     2281 cacgggagcc gcggcgggga ttggcgccgg cattgccttg cgatttgcag aggctggtta
     2341 cgccgttgcg gcgttcgaca tcgacggtgc tggcgcggcg cgcatggcgg aacagctccg
     2401 aggcttggcg ccgtcagttg cgatcggcgg agatgtttcc cgcgaagatg atgcgcaagc
     2461 agcggcggaa aaggccgtga gcgaattcgg ctcgctcgac gttctggtga acaacgccgg
     2521 aattgaggtc aacggttcag tgagcgattt gacctccgag gcctgggatc gccagatcgc
     2581 ggttaatctt cgcggcgtgt ttctgatgtc gaagtatgcg atcgcgaaga tgcgtggacg
     2641 cggaggtgca atcgtcaaca tttcgtcggt ccacgcgttt gtttcgtatg cgagctgcgc
     2701 tgcctacgac acgaccaagg ccggactcct cgggctgacg cgcgcgatgg cgctcgatca
     2761 tggcgtcgag ggtattcgcg tgaacgcgat ctgccccggc tacatcgata ctccgttgat
     2821 ggaccgctgg ctcgattccg ctcccgatcg cgaagagacg ctgcggcaag tgctcagcta
     2881 tcatccgctc ggaaggatgg gcactccccg cgatatcgcc gacgcgaccc tgttcctcgc
     2941 atccgatgcc gccgccttta tttcaggtgc ctcactggtg gtggatggcg ctatgagcgt
     3001 ggcggggcat tgaacggtgg ccgcggtatg tcagtgctta aatcgacatt cgcatgaggc
     3061 ggatctgtcg cttgacggaa gttaatcgat tctggcgttg gagaccttct caccgtacga
     3121 atttacgtct ttgtaaaatt tgtgactgga gcggaaagta ctgccaaggc gaagtgggcc
     3181 gaaccagacg ttgatggcat cttaaagtta cagttgatga aagtaacttt cttgaagtca
     3241 gccgggccac catcatactc aatccccatt ccaacaaaaa cgatattacg taacctatgc
     3301 tgatcgagct tcagaatagt tcgaggcccg cttgtgatga ttaaaagcgc cgggccttct
     3361 ttttgatcgc tcgtataggt cggtccaccg ccaatatgtt ctgcaatagc agcgcttgca
     3421 acattagcga aattgataaa tgaaaaagtg atgggatctt tttgttctat ggcttcccca
     3481 atgtcgaaat gaccaatctg gtcggacaag ggtatggcat acccaaggct ggaaagtctg
     3541 gatgaccgta gaaaagacac gtaatcgaga acttgagatg caactgccca cgcattggcg
     3601 tcctgcttga gagcggtcct tactatgcgt tgcccaacct ctgaaattga cgcttcatta
     3661 gaatgaatct tttctttggt tgctcttcga gccaccgcgc ctatcgtctt gaggcccaaa
     3721 tctaggttct caccactatg gagattgtcg ttcatgattg tgggaattcg tttacctggg
     3781 tctggcttca tgaactcgat tgtggtcgtt agcgttgcaa gcctttcgtt aatcgctgat
     3841 atcctttcgt ttacggcgtc actgtcatgc ttggcctcca cgggaaggtg cagaaagatc
     3901 cacccaaaca aaaacaaaat tacaattgtg gcagcgctaa ttgcggccca gccgatcgtt
     3961 cgatgccatt ttgatcgttc aatgagcgtc cctatttccc gttgcaagtc aggaatggag
     4021 gtgcttgagg ggttgggagt atcttttttt ccattgccca tgagtttgga cgaattctat
     4081 catgtcaaaa tggtggctcc gcccgcgtct gaacgaagaa acaattcaat gctatttgat
     4141 tacattgggc ggcgattaaa gagatgcgcg gccccattcc tcgcatccga tgccgcagcc
     4201 tttatttcag gcacctcgct ggtggtggac ggcgcccatg agcatggcgg ggcattgaaa
     4261 ataacacggg ttggattggg attagcgggt gatgatagga ttttgggcag gctgccggat
     4321 gcgaatagtc agatacagag agtctccctc gggatgcgtg cgtataagag ttgctctctc
     4381 gctgttaata ctgccagtcg cgtgtgcttg ccatttgcgg gccgccaagc tactcgatat
     4441 cgcgccatcc gggcaactca cgacgtggac agatgcggga ctgcggcatc tggcaaatgt
     4501 tcacgcggct gtcggagcct cgaatctcgg cgtggaatgg gacgaagaac gcgatgtccg
     4561 cgagattcgt gttcgttata acggcacctc cgcgaaaggc gcagcggtcg agtattggtt
     4621 caaatattgg ccgtacgatc caccgaaaat gcccaccatt gaagatccca tggacgatcc
     4681 gtggcaaggc aaatggttaa aggcggaaac aacggagatt tgcgatggag cggaatgccg
     4741 ctacgacttc aagccgctcg cggaaaccga aaacccgcgc gcggacaaac tgcccggtgt
     4801 cgtctaccgg cgaacgctca aggcgcggct gatctatgca ggcggaattc ccgacatcgc
     4861 ctcgctagac gtcttcaccg gtacggagca aaaaccagtt aacctccggg tagaatttag
     4921 tccgggcgag atcaccgcgc atcggaccag cactgcttcc gtgtacaacg gcattctgcg
     4981 ctcggtgaag ccatggcaag tttcgaaagg cttgctgctc gatttgattg cggcaaagcc
     5041 aaatctgcct ggatcgcacg atctgacggt cgtgacgatt cacgcgacgc caacggatac
     5101 gttctcattc cgcgtagacg atctcaaaca tggaccgatc tcaatcccgc agttccacgc
     5161 gcggattgtc gacataacac caggcgcgcc gcaagccccg gccgcacccg ggaaattacg
     5221 tgtaagggag agaattccca gggagccaga acagacatat cagcgcgcct cccgcgagat
     5281 tcccccgctc gatccatgga atcgcgaagg aggaggccgc gtctatctcc cgctggcgcc
     5341 cgattcggat tggcagaaat tcgcgtttga atatggcggg aatgtcttca ttagcaagcg
     5401 cggcattaag gcgaagggac gcgagttggc ccgtctgcaa tggaccggcg ataccatcac
     5461 gtggaagatc ggtacgggtg caacgccata ttaccgcgac gaccgcaaag tggcagtccg
     5521 ggttttggat ggctaccttc cagtggtgac acaacgctgg gaaaacgaag gcctccggta
     5581 tgaccaggag gcatacgcca ctcttctgcg cgggcctttg tctcccaccg gttccggccg
     5641 cgacgaagaa actccagcca ttctcatgat gcgtttggcg gctacgaatt ccactcgctc
     5701 agcgcgtaag gcggagatct ggctttcagt cgatggtggt ggaatcttga cggtcagcgg
     5761 gaaatccgtt tatgggaacg gcaaactgat cgcggaattg ggggaaaatc cgggcgccgt
     5821 tgaaacgaaa gcacactccg ttcacatcag cttcgacgtt ccgcctagcg gatcaaatgc
     5881 ggttgtgttc aaattgccgg ccgtttcgga cctgaatgct tccgatatcg aagacctgaa
     5941 tcgacttcac tattcatccg agcgacaaca actaattaca tactggaaga gcgtgatcga
     6001 tcccgctgtt cggttccgag tccctgagcc caaattcaac gctttagccc gcgcggtcat
     6061 cgcgcacatt cacatcagta ccactaaaga tccgaaaacc gggttatata tggttccggc
     6121 ggcaagttac tcctacgacg aattcgaaaa tgaggcatgt tatcaggtct tactgctcga
     6181 tacgctcgga cagttcgata ccgccgcatc gtatctcgaa ccgatgatgc gtctgcaagg
     6241 cagccaggac tttcccggag cgcacaaagg accacaagac gcgatcttcc atggtacaaa
     6301 gatcgatgac gtctataact acactgagca gggctacggc ctcgatcatg gcacggtgct
     6361 ttggacgttg gcgcagcact atctctatac ccgagacaaa acttggctcg aacacgcctg
     6421 gccgcacatg aagaaggcga tcgattggat tgtagagcag cgctccacaa cgaaactgac
     6481 cgatatccac gggcagaaga ttcgcgcata cgggctgctc ccggcttctc aacttgaaga
     6541 caatgcggac tgggcgaact ggttcccggt aaatgcctat gcgtgggctg ggatgtttct
     6601 cacttccgaa gcactggccg acattgggaa tcctgaagcg gcgaatattc gcaagaaagc
     6661 ggatgagttt cgagcggatc ttcgctccgc gattctgcgc tctgtcgaag ccgcgcctgt
     6721 agtacgtatg caggacggca cttatgagcc ctacgttccg gtgctgccga cgcgacgctt
     6781 tcgcctgttc ggccccatcc gcatggatta ttatcagcgg tatccaggcc cgcgtattaa
     6841 gcctcttctc cggctcggcg cggatcgcga tacgctatgc ggcgcggtcg tattgctgat
     6901 actgaatgtc ttctcttcca acgagccaat cacaaactgg attctgaacg actgggaaga
     6961 taatgagacg ttgagcagcg gaatgggaat gaacatccat ggcatgaccg acgaccgcta
     7021 ttggttcagc cagggtggta tggtgtttca ggccaactta gtcaacccca tccaggtata
     7081 tcttcgtttg cacgaagcgc aggcagctat tcgcaactta tacaacgatt ttgtcacctg
     7141 cttatatccg gaggtaaatc aatttacaga ggagtttcac cagtggcgcc acggtagcgg
     7201 tccgttctac aagatttccg acgaatcgcg ttttgtcaat cgagttcgcg acacgctggt
     7261 tttcgaagat caggatagtt tgtggcttgc ggccggaacc ccaaggcggt ggcttgattc
     7321 aaaagagggc attcacgtga atcgtgtcca gacattcttt ggcccagtga gttatgacat
     7381 acatcgcggc cggcggccgg ggatgctgga ggccacggtc gaacttccat ccagccggcc
     7441 gccgaaacat gcctggctgg tggcgcgggt cccttcgggg cacatccaaa gcgtcacggt
     7501 aaacggaaaa ccttggagta agatcaatcg ccgcctggag gccatcgaac ttccacaaca
     7561 acagggtttt ttgaaactgg ctatacgata ccagtaaccg ccctgcggcg cggctgcatt
     7621 ctgctcttca accactgatt caatataaat aacagcattc cgagcggtac cgcgagaaaa
     7681 tatggccagt aagtcactaa tggtggatcg gcggtaagta cggaaatact caccggcacg
     7741 gcgatcgtgt ttccattcgc ttgcacgtgt acgtctaagt gccactctcc cggcgatggc
     7801 agtattggct gagcggtgta taacagtttg tttgcggatc gtgtccgtgt agccgcaatg
     7861 cggatctccg gcccacccgg cttcgacaga cgcaaatcga tatcggcatc gagctcggga
     7921 cttccactcg ccgccgtttg caccataacg ctcaaatccg ctgcgccaac ccgtaacggc
     7981 acggatgtac tgaaaactgt gacgatcaga gagccagtgt gaacccgcga cagcaccgct
     8041 ccaccatcgg cgatcgaaag gctcgcgatc aggtgtaaac aagctgcaag ggccgcgatc
     8101 ctatttataa aaaccctcac ttcatcacgc cgcgcatttg cataggttga agaagaatcc
     8161 atacgcccag cccatacagt ccgcacgaga ccaacgccca aggcgtaata aggccgatcg
     8221 cgcggccaac atacttcgaa tattgccgcg ccaatcgcca aagaacatac aaagttaata
     8281 atagcccggt atccagtaac agcaactgcg cgggtgtaag ccaaattatt gaagcattcc
     8341 aggcggtcgc gaagtgatac agcaagtgtg ccgcccacat cgcgacaccc agcggcacga
     8401 gcgccaacgc gaatctccgc ataacgtcgg cgatgggaat cgacgcgtcc ataatcctgt
     8461 tcaacacccc acataaacaa accacggcgc cgggaagaat gacgacgcct ccgacgatca
     8521 acgcggcaat tacaagcggc attgctccag ctcctagccg cgcgtgccaa ctatgttccc
     8581 acgccataac cggcatgacc atcccggccg catttacaaa agcgccaaaa atcattatga
     8641 tcgcgaacac cgcaatgtcg gtgcgctttg acaatttacc gatcgaggac cgatccggat
     8701 cgagtgttaa cgtactcgct ggcacaacgg ccttcaacgc aacattgtca tgcgggcaag
     8761 cttttacgca atcgagacaa aatgtgcaat ccagatttcc gatctttttg ggctgaaaca
     8821 ggtccagttc acatccgtgg tcgcggtcgt tgccccggat gcaatcatgc gtacggcagg
     8881 aatggcaaac ctgcgcgctc ctgatcccaa tctctcctgg agagatcaac gaacttacaa
     8941 aatgaaattg gccaataggg caaacatatt tacaaaaact agcgccgcga aatactccgt
     9001 ccactaccag gatagttgcg aagtacccgg cgacgatcca tgccgtaagc cagggactgt
     9061 cccataagct gagagcttcg taggcccaca aataaaaaac gaacagagcc gcggggagcc
     9121 acttagtccg cagcaaacgc ggccagcgca accgggcggg gaaaaccaac cgccccaaat
     9181 cgcgagccaa cgtgaacgga caggccatac agaacaaatt gccaaccagc aacagtgcca
     9241 gaagagaaag agcgcgccag tgaatccatg gcaacgcgcc ggcaagattc atcggggcta
     9301 cttgcgttcc ccggaatccg tccgccatca cggcgatcgc gagcagtaac attgcgctct
     9361 gagccgcccg ccgtaatttc ggcagccggg atttcgttgt ggtacgtggt ctgcgtatcg
     9421 ggattcgttc cgctataagt tcgcgggatt tcaccattgc ggttccggag aattggtcca
     9481 cggcgataac gatggccggc agaaggtata ctagcgaacc cggcacccac ataatcgctc
     9541 cggcggtcgc ttggtcgtga aggccgctga taccgcccac ccgcgccgtt tcgtaaaccg
     9601 ggtagagcac tcggcccgaa aagataaaaa acgcggagag cgccgtgttg atcacgtccg
     9661 caagcaacag gtacggtatc atcgcccacc gcggccagcg cgagcctccg tgccctgatt
     9721 cgatgaccgg ccaccaaaag aaaaccccgg tccagaagaa acaagcgtgc tgagcgctat
     9781 gccatccggg agatcgcagc gccaattgat aaaattttgg aagatgccag acgatcgtac
     9841 ttaccgcgag cgttaaccat gcgaccggcg gtgatattaa ccatcgccaa gccgtcctta
     9901 gcaaaggcca agataaaaat ggaacgagcc cttccttcac gaacttacgt ggcagtcccc
     9961 gcaacaacgg aaccaacgga tgacccaata acagcaatgg cggcgcgatc atcatcagca
    10021 gcatatgctg cgtcatatgc gctgagagaa acagagaatc gaacgcgtca agcggcgatt
    10081 ccagcgctaa aaatagaatc gccaaaccgc agagaaaagc cgctaggcga gtaaggtcct
    10141 tctcgccgcg aatcagccgc cgcccacgta gccaaccgag aacgtacact accccagcgc
    10201 caagcagaat cgcaacaaca cgcgggtcga gcctccagga agatagcgcg gcggatacga
    10261 tcgtatccat catttacggg ttcagcttcg gttgcgccgg ctcagcggag tcacgcactg
    10321 ctggcacggc cgctggacgt aacgtaagca tgaaagaaac caatgcttct acttccgccg
    10381 gctttagact ttttccatag gcaggcatat tccccccacc ttggattacc tgccggatca
    10441 tttgcgcccg cgtaagacgc gtcgccactt catcgagcgc cggccctctc tcaccgcctt
    10501 ttccgcccag gctatggcaa tttctgcatt gcttgttctg cagcagtatc gcgccgtgta
    10561 actgcaacgg cgtgcgtcca ttcagatatc gcgggggaat cgcattactg gtccatgccg
    10621 acatttgcgg agaccagggc gacgtcttac ccatatatgt caggactccc agaaccagca
    10681 taatgacaat cacgctcagt accgcgaccg gtcttcgcct cgcgcttttc tcgccatccc
    10741 cccagataaa tggcagcgcc agaagaaaac caattagcac agccggtgcg acaaacagta
    10801 caagtacttc catccaatcc ggcagaagcg cgagagccgc gaagatagac aagaagtaaa
    10861 agtcaggccg tggattggca tctacgattg tcggacttgg ggggcccgaa ggaccgttag
    10921 gcccgaacac cgccgcgcac gtcagaatcc caacgatcac aatggctccg aaaataagat
    10981 ctttccctac cgcattcggt acgaacggaa cacctttttc ccgcagaagc tccgcatact
    11041 cagcgtcgta agtcgactta ttcactctgt accccggttt tggatattca ttaattccct
    11101 tggtgagtac caaccggaga tgcatgctga ctacggcaat gatcaggcca gggataataa
    11161 atacgtgcag cgtaaaaaag cgagacaggg tctctcctgc gataatcggc ccggccaaca
    11221 atacccgtac taactcagcg ccgatcaagg gaacgcggcc ggtgatcgaa gctccaattc
    11281 ccaagcccca gtacgcgtcc tggtcgaaac ggaggatttg cccagtgaag gccatgccca
    11341 gagtaagcaa tagcagaaac actccgctga tccatgtaag ttcgcgagga tacttatatg
    11401 cgccgaatag aaatacctga atcatatgta gcgtcatgat cgctatcatg aagttagatc
    11461 cccagttatg aactgcccgg agataccagc ccaggtattg cgcatggttt agatattgaa
    11521 ggcttgtcca ggcctcattt gtagacggaa cgtaaacaaa tgccaggcac gcgcccgtaa
    11581 taacctgaat cacgaaacag agtagtgtgg cgcttccaaa gacataaaac cagctactcg
    11641 cgcttgccgg gaccgaatga cccgcagtcg acgcgaatag agcggtgata tgtagccggc
    11701 tatccagcca gaccgccgtt ttcctcagcc acttcattcc gtttctccgg cttatacggg
    11761 attcgcgagt gtgggcattt gtccgccctt cacccacaac tccccattct ggactttata
    11821 gtcatattcg aacaatccac gcggcggcgg accggaagcc cgggatccat cctcatagta
    11881 gacgccgccg tggcaagggc acataaaaag ctgcgattcg cgaaaccagc gcaccgggca
    11941 tcccaagtgc gcgcagttaa tcgcgaagac ctgaaagtga ttacccttaa tgtgacgaac
    12001 ccagcatggg atgtgagccg tttcgccgtc ccagggacgc gtaaatggat tctggtaagt
    12061 ggcaagacgt gtctggcttt cgggaaagta ttccagacgt cctaacgaaa tccacttacg
    12121 gggatctctg cgattagcga agctggaaaa gacaaatcca atcaacggta ccgcgagcag
    12181 ggctgcggcg accacatcca gcgccgcacc ggcgagaaat agaacgtggc gtcgcgttgg
    12241 aggcgttgta ttcgcgttct cacttcctgc tccatgacta acatcgctca taggtactcc
    12301 ttaataatgc tattgggtct cacgcttatt cgccgctccc gtcggtcgta gcgaaaccaa
    12361 atacgaaacc aagtcactaa catcctgatt gctcaacgcg cggcccatat tgagatggcg
    12421 ccaatccggc atcccacgat cgtgacgccc ggcgatgata gaactgcgta gccactgatc
    12481 gcttacgagc ataagatacg aggcatcggt aaccggccca atcggcgcgc cagttccgtg
    12541 acaggcgaaa caatcccgcg tgaataaccg cttgccggcc acgggatcgc cggcatcatt
    12601 cgtcgcgcta tagggcgggg gcgtagcccc cccaaggctt accggcttgg cccattcgcg
    12661 ctctatccca cttactaatg ccttaatatc gttcggatcg agcgggcctc catcgctctc
    12721 ggcgaaggcc ggcattgccg ttcccggacg gccatgttct atcgtgtctt cgagcgtgtc
    12781 tttcggaata agcgcgagat agaatggatc gttcaaaggc cgtccggggc cgtcctttcc
    12841 gtcattaccg tgacagccgg aacagttctc gttaaaaagc gtatggaaat cgggctcgct
    12901 cttcgacgcc tgagcaggtt tcggcttgcc acatcccgcg acgcccataa ggaacagaca
    12961 tatcgccaat ccggtgaatg cgctcattgc cggtcttctg gggggcgcgg cgaaattccc
    13021 ggagcatcga tgaaggcgcg gtgcgcgaag ccccacgatt gcatcgtgtg aatgctcgtg
    13081 cttcgcgcga tgacgaaccc gcccaccagc ccgaatccaa gttggcagac cacaaaccaa
    13141 ggccagttga tgcgttcatt cagcgccggg tccaacatct gcaatgccgt cgcgatcagc
    13201 ccgctccaga aaagcggcat tagaatcccg gcccatattg gggcgtgttt cggaaacatc
    13261 ggcagaatta ccgcgtaaac gacacccacc agaatcgaca ggacggtatg acccacgcac
    13321 gccgcgacga aagcgagtcc gttgaatgct cgtaattgtt cgaggctcgc gctgctgata
    13381 gacggaatca cgactccggc caacaggtta atcggatacc agatgctatg ttgagccaca
    13441 aggccgtaca agcacgcgag cgccgccatc acggctccgc cggccaatcc gccccaaagt
    13501 ccggccgaat atggatggat cctttcggga atatgcgctc gatgttgtcc ttcgccaacc
    13561 tgtaaacgaa ttaccgatcg cgcttcaacc atgatcggat cggggcggcg gtcctgctcg
    13621 atcgcaacct gctcatgctt ctcgagcgga atcacgtctc gccaccagcc tatggccgaa
    13681 cgtatcgcga tgacgaggcc caccaggctc acgacgaaat tcgtgaccag ccccccgagt
    13741 aggagcgcca agccgaacgc aaggaccata ggccaaaaag taggtgcggg catctcgatg
    13801 tgcgtctgtt cggtccgctg tcctgcctct ccgtgaccct cgtattccat gtaagtatct
    13861 ttacctcccg atgagataga cgaccgagaa cacaaccacc cacacggcat ccacgaaatg
    13921 ccagtaccac gaaaggaaag cgacgcggcg ctcctgggtc tggatgggaa atcccgacag
    13981 cgtaagtata agaactaaca acaggaaagt cattccaaca atgacatggc tcgcgtgcag
    14041 cccgacgagt gagtagaacg ttgtgccgaa caagttcgtc gcgatcgtca ggtggtcaat
    14101 gtaaatcaga tgatgccact cgacgccggt cgaaataagg aactcggcgc ccagcaaaat
    14161 agtaactgtc caccataact taaaacggcc aagctggttg cgtttcagtg cgcgttccgc
    14221 cattatcatt gtcaagctgc tcgatagcag acaaaccgtc gccacgatcg gaatatcgag
    14281 aacatccttc gggtatggtc cgctcaggtt cttgccaatg taataaaggt acgaaacaat
    14341 aaaaatcgag aagagcgatg attccgtaat gatcaggaac ccgatgccga tcattccgcg
    14401 gtcgggaagt ttccactcgc gttccaggtt ctcgggactc gccgggacta aggtggatgt
    14461 cgccatgata cttactcgaa cttccaatcc ggatcttgcg gatgcttaag atcccacaaa
    14521 ggccggctgc tccgcacgac cggaagcttc tcgaagttat attccggagg cggcgaactt
    14581 gtggtccatt cgagcgtcca gccatcccat ggatcgtctc ccgccggctc gcctgagcgc
    14641 agcgattgaa tcacgttcca aatgaagcat gcgatcccag cgatttgaaa gattactccg
    14701 atggacgcca ccatgttcaa gagatcccac cccctcccag gcgcgtaagt atatattcgc
    14761 cggggcatgc cgagaaagcc cagaaaatgc atggggatga aagtcatgtt gaagccgacg
    14821 cagaacagcc agaaatgcca cttacctagc gtctcgctca tcatcttgcc ggaggctttc
    14881 ggaaaccaat aatacatgcc tccgaacaaa gcgaatatta ttccgccaat taggacgaag
    14941 tgaaaatgtc ccacgacgaa ataggagtcg tgtagctgcc agtcgaatgg cgccacacct
    15001 agcatcacgc cagttagtcc agccaccaga aattggaaaa ggaacgcgca gcaaaacaac
    15061 atcggtgtcg caaaacgaag ttttccgccc cacatcgtcg caagccagtt gaaaatcttg
    15121 attcccgtcg gcaccgcgat cagcatggtc gcggcgacga aaaacatgtt cgctgtggac
    15181 gtcatgccga ccgcgaacat atggtgcgcc cacacgctca tgctgatgaa gccgatcgct
    15241 actgccgccc caaccattgc ctgtctgccg aaaatcggct ttcgcgagaa gaccggaatt
    15301 atttcggaca tcacggcgaa agcgggaaaa acaaggatat agacttcggg gtgcccgaag
    15361 atccagaagt aatgctgcca caggacagcc gatcctccgg cctgggtgtc gaatgcatgg
    15421 gccccaagga aacggtccag caacagcatg atttggcagg cggagagtgg cggcagcgct
    15481 agaatgatca tccaggaaac gaccagcatc atccacacga atagtggcat cttgatcagc
    15541 gtcatgccag ggcatcgcat ggacgcgatc gtggcgatca cattgattgc gcttgaaatg
    15601 ctccccacgc ccgtcaacat aatcgcgagt atccagaagt cggtgcttgc gcctcgcgag
    15661 aatgcgcgct ccgtcaaagg cgagtaagca aaccacccta catccggcgc cgatcccgct
    15721 cccgaaagcc cgccaccgcc gatgtaactt gagtaaagta gcaggccgcc gaacaggaag
    15781 gcccaaaaac cgaaagcgtt cagtctgggg aaggccatgt cccgcgcgcc aatcatcagg
    15841 ggtactaaat aatttgcgaa gcccgccagg atcggcattc ccaacagaaa caccatcgtg
    15901 gttccgtgca tcgtgaatag ccggttgaac gtttccggcg atacgacatg ggcgtccggc
    15961 acggccaatt gcagccgtat cacggacgcc atgatgccgc cgatcaccat gaaaatcagc
    16021 gacgcgacga tgtacatcag ccccagtcgc ttgtgatcca cggtcgtcac gcactcatag
    16081 agcgtttccg agagagagaa ctccgcgtgc cggtggctcg gatactccag tggataatcg
    16141 ctcgcggcca taaacccttt cgcctatttc aaagactcaa gatacgcaac cacctgatcc
    16201 agttcctgcc cgttcaatcg catgctcggc atcagacatc ccggtttgat ggcctgcggg
    16261 tcttttaccc agtcccgcag attcttcggc gtattgggca gcacgcccga gccaatcgtt
    16321 tcgcggctca tcagatgtgt cagatccggg ccgacatcgc ctttcgcgga cgtgccccgc
    16381 acggtgtggc aactcacgca cgataacgat tcaaacatcg ctttgccctg gctcaccgcg
    16441 ggattgtcct cggccggctt gctctgcgcc gcggcccatt tctcgaaatc gccctcgcga
    16501 tgcacaatca cgcgcagcag catgttcgca tgttgctggc cgcaatattc ggcgcagtta
    16561 cccaggtaca cgcccggctc tctcggatcg aaccacaacg cattatcgcg gttcggaatc
    16621 agatctgtct tcccggaaag ttgtggcacc caaaaactat ggatcacgtc catcgattcc
    16681 agcttcataa atgtcgcgtg cccggaggcg tccgcaaccg gaacgtgaaa ctcgttcgcg
    16741 gtcgtaatcc ccaggtcggg ataatgaatt tcccaccacc actgatggcc cacaatcgtc
    16801 gcgcgcagcg cggacggccc aggctgcgca tcttgtatcg cgccaatcat ccggacggtc
    16861 acgccaagca ggatgaacac gatcaggatc ggaatcaccg tccaagcgac ttcgatttgg
    16921 ttgcttccgt atacttgcgg cggttcctgc cgtgcgtcgt ctccaggctt gcgccggaag
    16981 cgaatcatcg cgtaaagcag aagccctccg acaataacga aaatggctcc cgtgatcgac
    17041 agcacaaaaa gcgccagctt gtattcggaa tcggcgggcg tcgccagcgg ccggaagatg
    17101 ttcgcgatcc gatgctccgc gagcgccgga acaccggttt gcagcaaagc ggcttgagcc
    17161 agcgcgagca ttcccattcc tccgttgcgc gacgacaatc ataatttcaa tcgcgcatga
    17221 atgcaaccgc tctacgccat gtcactcagc cgcgcccgcg aattgaatgc tgctgcggta
    17281 gtctgatctt tggaaaacgc cgcgaggaaa tcgaatctga gccggcttct tccgttacga
    17341 atcgcagccg tcctggcgat tgccgtcaat ttcttctggt tcaccgccgg catcttcggc
    17401 tctcatttcg cgcccgatga aatgatgaac atgggttggg cctgggaggc ggggattgcg
    17461 aaaatcctcg cgaacctggc ccagttctac accaccttct accgtcccac gggcgcgctc
    17521 tattactgga ctcttcaccg cctcttcggc ctcaatcccg ttccgtaccg tgtgattgat
    17581 ctcgcggttc tcgccttcaa cgtatggctc gcgtatcggc tggcaagcgt cctctcccga
    17641 tcgcgatccg tcgcgtggct ggccgcgttt ttgttcagct atcacgaaaa gatcatgatc
    17701 tgggtcgctt acaacggcgc atgggtttac gatcgcctct gcttcacatt ctcaatctcg
    17761 gctctgcttg tttatgtgcg tgcccgctca cgcggcggcg agatcagcct ccggagaggc
    17821 gcggccatcc ttgttcttta cgtgctcgcg ctgggatcga aagagatggc cgtcgcggtc
    17881 ccggtgctgc taatcgcata cgaatgcgtc tacgcgttcc ggacttggcg ccagaaccgg
    17941 aatgcctggc tgttgataac cctcctctgt gtaatgacgg cgctttacgc ctacggcaaa
    18001 acgcacgggc cggattcgct catcaacaat ccccaatatc gtccggttct taacgccgcg
    18061 acttatctgc gatcccaagc gcggcacatg caagactatt tctttctgaa ccatgccttc
    18121 tctccggcga tagccgcgtt cattctcctc gcgatggccg ccgcggcgtt tgcattccgc
    18181 tcccgcccat tgatctatgc gatcgccttt ctgatcttca gcccgctccc catcgcattc
    18241 atcaaccgct ccggcggatc gctctacatc cctctgttcg gttggtgcct
//
LOCUS       DATNDT010000269         3732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2326829, whole genome shotgun sequence.
ACCESSION   DATNDT010000269 DATNDT010000000
VERSION     DATNDT010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2326829"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..546
                     /locus_tag="VN788_08705"
     CDS             <1..546
                     /locus_tag="VN788_08705"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HXU20647.1"
                     /translation="VQGMLDRDILRLMMRNAGITDVAMRRVMPDILQSAQSIYVRRCP
                     DLRTRVCPGVRMALTRICRRGHVTGLVTGNLTRIGWKKLERAGLKDHFRFGAFAELAN
                     DRAGLVRIAVREARREGWIERGSPITLVGDHENDILAAKANGIRSIAVATGISTAEEL
                     ALIAPDLLLPDLRSLRLDMLQ"
     gene            543..893
                     /locus_tag="VN788_08710"
     CDS             543..893
                     /locus_tag="VN788_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20648.1"
                     /translation="MKIAIFLLVSTLLLLAGCAPPGHPEITAIIGATVQNPNHPPLTD
                     SIVVIEGSRIRAVGKQSTVPIPKDAETIKGYGGVVKPYAMGMIAPGQPANLTLAVGSR
                     IRVMQKGEWLPESR"
     gene            complement(856..>3732)
                     /locus_tag="VN788_08715"
     CDS             complement(856..>3732)
                     /locus_tag="VN788_08715"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HXU20649.1"
                     /translation="DAVFLERFIRRARHIEIQILADKHGNVLHLYERDCSVQRRNQKV
                     VEVAPAVNLNPAIRNALADAAVRLAKGSGYYNAGTVEFLVDADSGEWYFIEVNPRVQV
                     EHTVTEMVTGVDIVRSQIQVAQGFELHGPEMNLPGQQDIPLHGFALQCRVTTEDPANN
                     FVPDYGKIHTYRSPAGFGIRLDGASAYGGAVITPFYDSLLVKVTAWGREFSHACQRMD
                     RALREFRIRGVKTNIPFLENVVNDPRFQKGEITTSFLAENPSLFRFTPRRDRATRLLT
                     YLGDVIVNGNPEVAGKSKPKGLKTPPVPEHIAAAPPAGTRQLLQQLGPEKFAEWTWHQ
                     KRLLFTDTTFRDAHQSLMATRVRTYDMMAIANFAAHRLHNLFSLEMWGGATFDVSLRY
                     LHEDPWLRLQRLRAAIPNICFQMLLRASNAVGYTAYPDNVISAFVERAAEEGIDIFRI
                     FDSLNWLPNMRVAMETVRKTGRICEAAICYTGDILDPKREKYSLDYYVRLAKELEKMG
                     AHILCIKDMAGLCKPYAAHKLVKVLRNEIGIPVHFHTHDTSGINSASILKAADAGVDV
                     ADGALAPMSGTTSQPNLNSLVAALANTPRDTELDMDALNQCADYWEIVREYYKPFDNA
                     PLSGTAEVYLHEMPGGQYTNLKAQAESMGLGARWHEIARAYADVNMAFGDIVKVTPSS
                     KVVGDLAIFLVSHNMTIQDLERLGPDHNLTLPNSVTEMFSGSLGEPEGGWPPKIQATV
                     LRGAAPKPGRPGEHLAPVDFAETAALVEKKVGHKVSPTDLLSYLIYPDVFVKFAKTRS
                     TYGTVDVLPTSQFFYGLEKGEEVAVELEPGKILIIKFLTMSEPQPDGTRTIFYELNGQ
                     PREVTVRDRSRKAVVEARQKADTAIPGQVGAPIPGAVTAISVEIGQQIAKGDRLLVME
                     AMKMQSTIYAPVAGKVTQKLVNIGDKVESKDLLIVIQEATPLSA"
BASE COUNT          769 a         1066 c         1097 g          800 t
ORIGIN      
        1 gtccagggga tgctcgatcg cgatattctg cggctcatga tgcgcaacgc cgggatcacc
       61 gatgtcgcga tgcgtcgtgt gatgccggat attcttcaaa gcgcccaatc gatttacgtg
      121 cgccgctgcc cggacttgcg aacgagagtt tgtcccggag ttcgcatggc gctcacgcgc
      181 atttgccggc gcgggcacgt cactgggctg gtgactggca atctgacccg catcggctgg
      241 aagaagctgg aaagggccgg cctcaaggac cattttcgat ttggcgcctt cgccgagctg
      301 gcgaacgatc gcgcgggact tgtgcgcatc gcggttcgag aggcgcgtcg cgaaggatgg
      361 attgagcgcg gcagtccgat cactctcgtc ggcgatcatg aaaatgacat actcgccgcc
      421 aaggcgaatg gaatccgctc catcgccgtt gctaccggca tctcaaccgc ggaagagctt
      481 gcgctgatcg ctccggacct gcttctgccg gatcttcggt cgctccgttt ggacatgttg
      541 caatgaaaat cgcgatcttc cttctcgttt cgacgcttct actgttggcc ggttgcgccc
      601 cgccgggaca tccggagatc actgccatca ttggcgcaac cgtgcagaac ccgaatcatc
      661 cgccgctaac cgactctatc gtggtgatcg aaggcagccg gatccgcgcg gtcggaaaac
      721 agagtaccgt tccgattccg aaagatgctg aaacgatcaa gggctacggt ggtgtggtaa
      781 agccgtacgc aatgggcatg atcgcgccgg ggcagccggc caatctcacg ctggcggtag
      841 gctcccgcat tcgagttatg cagaaagggg agtggcttcc tgaatcacga tgagtaagtc
      901 cttcgactcc accttgtcgc cgatatttac cagtttctga gttacttttc cagcgactgg
      961 cgcgtagatc gtcgactgca tcttcatcgc ttccattact aacagccggt ctcccttggc
     1021 gatctgttgt ccgatctcga cggagatcgc ggtaacagcg ccgggaatcg gcgcgccgac
     1081 ttgccctgga attgccgtat cggccttctg acgggcttcg acgacggcct tccgcgaccg
     1141 gtcgcgtacc gttacttcgc gcggttgtcc gttcaactca tagaagatgg tgcgcgtgcc
     1201 atcgggctgc ggctcgctca tcgtcaggaa cttgatgata aggatcttac caggctctaa
     1261 ctcgacggca acttcctcgc ccttttcaag accatagaag aactgcgagg tcggtaagac
     1321 gtctaccgtc ccgtaagtcg agcgtgtttt agcgaatttc acgaaaacgt caggataaat
     1381 caagtaacta aggagatcgg tcggcgagac cttatgcccc accttctttt ccacgagcgc
     1441 ggcggtttcc gcaaaatcga ccggagccag atgctcgccg ggacgccctg gcttcggcgc
     1501 cgcgccacgc aataccgtgg cctgaatttt cggcggccac ccaccttcgg gctcgccgag
     1561 cgaaccggaa aacatctcgg tgacggaatt gggaagcgtc agattatgat ctgggccgag
     1621 ccgctcgaga tcctgaatag tcatgttatg gctgaccagg aagatcgcca agtcgccgac
     1681 caccttactc gacggagtaa cctttactat gtcgccgaag gccatattta cgtctgcata
     1741 agcgcgggcg atttcatgcc agcgcgcgcc aagccccatc gactccgcct gggctttcag
     1801 gttcgtgtac tgaccaccgg gcatctcgtg taagtagact tcggcggttc ccgataatgg
     1861 cgcattatca aagggcttgt aatattcgcg gacaatctcc cagtaatcgg cacactgatt
     1921 gagcgcgtcc atatctagct cagtgtcgcg tggcgtgttg gcaagcgcgg caaccaggga
     1981 gttcagattc ggctggctgg tcgtgccgct catcggagcg agcgcgccgt cggcgacgtc
     2041 cacgccggca tcggcggctt tcagaatcga tgcggaattg atgccgctgg tatcgtgcgt
     2101 gtgaaagtga actggaatgc cgatttcatt gcgcaaaacc ttcaccagct tgtgcgcggc
     2161 ataaggcttg caaaggcccg ccatgtcctt aatgcaaaga atatgcgcgc ccatcttttc
     2221 aagttccttc gcgagccgca catagtagtc gagcgagtat ttctcgcgct tcgggtccaa
     2281 aatgtcgccg gtatagcaga tggccgcctc gcaaattcga ccggttttgc ggactgtctc
     2341 catcgccacg cgcatattcg gcagccagtt cagcgaatcg aaaatgcgga agatatcgat
     2401 gccttcttcg gccgcgcgtt ccacaaacgc ggagatcaca ttgtcgggat atgccgtgta
     2461 accgacggcg ttcgacgcgc gcagtagcat ctgaaagcag atgttgggaa tcgcggcgcg
     2521 gagccgctgg agtcgcagcc acgggtcttc atgcagatag cggagcgaga catcgaatgt
     2581 cgcgccgccc cacatctcca ggctgaacag gttatgcaaa cgatgggcgg caaagtttgc
     2641 aatcgccatc atgtcgtaag tgcggacgcg tgtggccatt aacgattgat gggcatcccg
     2701 gaaagtagta tcggtaaaca gcaatcgctt ctgatgccac gtccattcgg cgaacttctc
     2761 ggggcccagt tgctgtagta actgccgcgt gccggccggc ggagcagcgg caatatgctc
     2821 cgggacagga ggagttttca ggcctttcgg cttcgatttg cccgcaactt ccggattacc
     2881 gttgacgatg acatcgccca aataagtaag aagccgggtc gcgcgatccc gtcgcggagt
     2941 gaagcggaac agcgatggat tttcggccag gaacgaagtc gtgatctcac ctttttggaa
     3001 gcgtggatcg ttcacgacat tttcaaggaa cgggatattc gtcttgacgc cgcgaatgcg
     3061 gaattcgcgg agagcccgat ccatgcgctg gcacgcgtgc gaaaactccc ggccccacgc
     3121 cgtgactttc accagcagcg aatcgtaaaa cggcgtaatc accgcgccgc cgtatgcgga
     3181 agctccgtca aggcggatgc cgaatccagc gggagagcgg taagtgtgga ttttgccgta
     3241 atccggaacg aagttattcg ctggatcttc ggtagtgacg cggcattgga gcgcgaagcc
     3301 gtgcagcgga atgtcctgct ggcccggcag attcatttcc ggaccgtgta attcgaaccc
     3361 ttgcgcaacc tgaatttgtg agcgaacgat gtccacgccg gtcaccattt ccgtaacggt
     3421 gtgctccacc tgaacgcgcg gattcacttc gatgaaatac cactctccgc tgtccgcatc
     3481 gactagaaac tcgaccgttc ccgcgttgta atagccggaa cctttggcaa ggcggacggc
     3541 cgcgtcggca agcgcgttgc ggatcgcggg attgagattg acggctgggg caacttccac
     3601 gaccttctgg ttccggcgct gaacagaaca atcgcgctcg tagagatgga ggacgttgcc
     3661 gtgtttgtcg gccaggatct ggatttcgat gtgccgcgcg cggcgaatga aacgttcgag
     3721 aaaaaccgcg tc
//
LOCUS       DATNDT010000270        10410 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_303204, whole genome shotgun sequence.
ACCESSION   DATNDT010000270 DATNDT010000000
VERSION     DATNDT010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303204"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="VN788_08720"
     CDS             <1..682
                     /locus_tag="VN788_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXU20650.1"
                     /translation="LAVETQKFFMMLGIPVLQVYGLTETTAICTMDDPRHVEPGRVGC
                     AIPGVEMKVGDHGEILVRGPNIFAGYWQRPEATASALAGGWFHTGDQGEADANGNWRI
                     TGRLKNLVILNSGHNVAPEPIEEKLASLLPEAQQVMLTGNQRSFLGALITIPGANGAQ
                     RERIQAVLDSLNADLPHYKQIRGFQVLGETFTAENGMLTTMGKLKRDAIAARYADEID
                     KVYAKRTA"
     gene            679..1482
                     /locus_tag="VN788_08725"
     CDS             679..1482
                     /locus_tag="VN788_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXU20651.1"
                     /translation="MKSFRGEALSWQVVDGAIELVLDRAPCNEIGSVALAELEKFVGA
                     LGVLERDAHALIITSARKEGFCAGADLRELYHRSSQLPAAARLEGVRDFLERIHRVMN
                     AIDEAPLVTIAAVHGVTFGGGFELALVCDLIVADKMARFCFPELRLGLIPGFGGIPRL
                     KRDLGNALVRDLLFTGRSINATKAQAAGLVSQVVAEGDALRAARATAAQIGKFDRETT
                     KAAKRFIKPIPHEELRREIEIFCELFTRPAVDAGLRKFVESTDALPYLP"
     gene            1500..1742
                     /locus_tag="VN788_08730"
     CDS             1500..1742
                     /locus_tag="VN788_08730"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HXU20652.1"
                     /translation="MANLQETTEQILDLAAAHFKVPRTQLSADDDFFKKLGINSLQAL
                     DLLTRLEQHFRIELPDYELQDVSDFRTLAERIQSRL"
     gene            1781..2152
                     /locus_tag="VN788_08735"
     CDS             1781..2152
                     /locus_tag="VN788_08735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxxExxY protein"
                     /protein_id="HXU20653.1"
                     /translation="MDRLNHLSGKIIGCAIEVHRVLGPGLLEATYEEALCIELAEFGL
                     THRRQWPIPISYKGRRLGKYRLDLVVEDAVIVEIKSVERFDPVFQAQVLTYLRAAEKK
                     IGLLINFNSRLLRDGIKRIIL"
     gene            2197..3906
                     /locus_tag="VN788_08740"
     CDS             2197..3906
                     /locus_tag="VN788_08740"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HXU20654.1"
                     /translation="MLDLNKYNCLGEALRAAIDRWPGDICLIESDRERENCRPTYRQF
                     SEAALPLAAALQDAGLSSGTRVAILMTNQSKWLISAYAVFYCGGVLVPLDYKLPADDQ
                     LKLLAHSKAEYLIIEFHLWRAITKAEKFGELSARTVLVTEAPANAELAGAQRWEEFRA
                     GREPEFVKRERKDPACIVYSSGTGGRPKGCVLTHENYLEQATAVAPIFPFWPGARYLS
                     IIPTNHAIDFMGGFIMPFTGGGTVVHLRTLRPEYIRDAFTRYGITYMAVVPLILKNLQ
                     RGLTEKFAALPPFKRRVLNLLVATNRALTKSKPRPSLSRKLLKDIHAAFGGGLRALLV
                     GGAFTEPATIEFFHDLGIPIYNGYGCTEACTAITVNDQRPFRPETVGGPVRGMEIRIL
                     NSGEDGVGEVAVRSKTVMSHYLDDPEQTAETIVDGWLITGDLGRLDDFGQLQLFGRKK
                     NMIVTEEGKNIYAEDIEAVFDGLAVKESCVFAANFLWPARTMVAERLVLVVHPEAGKQ
                     VNAELAEEIAERNRRLANYKRVGGYLVWERDFPLTASLKCKRGELAGQIREAVSRESI
                     VPL"
     gene            3940..4860
                     /locus_tag="VN788_08745"
     CDS             3940..4860
                     /locus_tag="VN788_08745"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HXU20655.1"
                     /translation="MNTEKTRFLAIVNPAAGGGRCGELAPAALERVRRALDAKDATME
                     IVYTKKAGEATELARAAYRERVRHFLAVGGDGTSYEIINGVFPESLENGRVSLGFLPL
                     GTGNSFLRDFTKKGVEYTMPAIAEGRSRVCDVIRGRHASGEIYFINVLNLGFPADVAE
                     LTNRRFKSLGETGYIFAVFARLAWLFYGTFPHRIDGHEWDRRPSLFLAFTNSKFTGGK
                     MMIAPKADSADGKLEYVRWAPIGRARLLCLFPRLFTGTHIDHPLATRAAAERVEFDLA
                     EPVNIVVDGENLKLNLQSLEILPGALDVIV"
     gene            4857..5633
                     /locus_tag="VN788_08750"
     CDS             4857..5633
                     /locus_tag="VN788_08750"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXU20656.1"
                     /translation="MKKAWLTVRSLILWAVSGVHFGVVCTFLVFLGIFVDPRKNDWPQ
                     RFFFRNILRFAGVRFEVRRAPGFDPKRTSIFICNHVNIFDPFVIYSAIPQFVRGFELE
                     SHFKVPIYGWMMGRFGNIPVPDTRSREGIERMTRKAKEALDSGVSLIAFAEGSRTRHG
                     HVQRFHKGVFRLAQRFGTPIVPMSIVGSYQFFQTGNWMLYPGKITVYLHDTIETADVE
                     RADIDQLAERVRNTVAGPVEESLKCVYTTQQASGSVLSGS"
     gene            5655..6116
                     /locus_tag="VN788_08755"
     CDS             5655..6116
                     /locus_tag="VN788_08755"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU20657.1"
                     /translation="MQTNVWGGDLAAWPFIQGEPQWESAREDATSLPTHMLGMTENGK
                     HNRRFPRIATPSGVWVAWQQDGQKQSVSRVCDLNIGGLFIATPKPASVGTVISVLFSV
                     PEGEIRGHATVRNFKAGEGMGVQFTAMGGQDTDRLRKLVARLLDAASSQQA"
     gene            complement(6156..6716)
                     /locus_tag="VN788_08760"
     CDS             complement(6156..6716)
                     /locus_tag="VN788_08760"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXU20658.1"
                     /translation="MVSGLAATGLTPNMFTFLGLAVNSWAAACFAMGHFRQGAAVLFL
                     AGFLDMADGQVARRVGRVTAFGAFLDSTLDRYSDLALYLGFVVHYTLIQRFFYMALAA
                     VAMASSFMVSYSRARAESLIPSCKVGFLERPERLVLLILGGLFNRMAQALWVIATLST
                     LTVIHRVVYTWRELKAGRALPGVTAP"
     gene            complement(7208..9562)
                     /locus_tag="VN788_08765"
     CDS             complement(7208..9562)
                     /locus_tag="VN788_08765"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXU20659.1"
                     /translation="MRIARTHPRATLWRAASLLACIALFATAYAVGAKQARPSKNHPR
                     ENTSSTKRGRPQPATSPPEDAAADQSSVFAGGIRDNNVGVALMDAHRFSEAFSRFQTA
                     CIMVPNSDTGCLNSGIALLAMRQFDQAQRILSTSANRDPKSATAWFNLGLLARAAGQT
                     QAALVDFQRVASLDREDAETQCLIGLSYMAEQQYDAAFKAFQAALKIDPLNATAESGA
                     TEALAKRGHAAGRQTADASATQAPDPALLSTFGDHYGEQGKYSRAAEIPPPDTVAPEV
                     PVHFVDVTRQSGLLFPSSTVAAHGRGARGGSRSTAKPAAPSEVHSMADFLGSGACVFD
                     YDGDGRPDIFLVDAGGNGGAALYRNLGRGRFADVTKAAKIDFQGAGMGCAVGDYDNDG
                     HPDLAISFNGGVRLYHNNGKGAFADVTAASGIQMDSLVMGLSFVDYDHDGNLDLYATQ
                     LSNAPLVNPSQPFVWPNDGAPGNIMWRNNGDGTFSDVTSALHLAGSASSTGAIATELT
                     GDGAIDFILTGLSPTPSMLMNGRGGTFKSASWWGAETQGPTAGAVAQDFNKDGYMDVA
                     LTHSQPTTLGLWRNVAGKGFQRLALPDPGWMRAWGIATLDYDNDGWIDLVAVGDTFSG
                     EGRIALLRNEGGKGFHDVTPETGLDKIALHDPRSVIAFDADGNGSLDLLITQNHRPPL
                     LLRAVGAEKHNWAQFALRGATQNRMGIGVRVEMISGALRQTWEVPAASGYLSQGPPLI
                     STGLGEEGVDAIRVRWSKTNVQVQLRPPANRKTQISQADAGKTP"
     gene            complement(9974..>10410)
                     /locus_tag="VN788_08770"
     CDS             complement(9974..>10410)
                     /locus_tag="VN788_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXU20660.1"
                     /translation="IRHPADIAAAREKTSETFTMMLGSVASVSLLVGGIGIMNIMLVS
                     VTERTREIGVRMAIGATEHDVRTQFLIEALALSLIGGFIGIIFGIGMSAGVSQLLNWP
                     TAISSLAIGAAVLFAAGIGVIFGYYPARKAASLDPIEALRYE"
BASE COUNT         2154 a         2755 c         3233 g         2268 t
ORIGIN      
        1 acttgcggtg gaaactcaga agtttttcat gatgctgggg attccagtgc tgcaagttta
       61 cggcctgacg gagacgacgg cgatttgtac gatggacgat ccgcgccatg tggaacctgg
      121 gcgcgtcggc tgcgcgattc ctggcgttga gatgaaagtc ggcgatcatg gggagatttt
      181 ggtgcgcggg cctaacattt ttgccggtta ttggcagcgg cctgaggcga ctgcgagcgc
      241 gcttgcgggg ggatggttcc acaccggtga tcaaggggaa gcggatgcga acgggaattg
      301 gcgcatcacg gggcgcttga aaaacctggt gattttaaat tcgggacata atgttgcgcc
      361 ggagccgatt gaagagaagc ttgcgtcgct attgcctgag gcgcagcagg tgatgttgac
      421 tggaaatcag cgaagctttc tgggcgcgtt gatcacgatt cccggcgcga acggtgcgca
      481 gcgagagcgc attcaggccg tgctcgattc actgaacgcc gatttgcctc attataagca
      541 gattcgcggc tttcaagtgc tcggcgaaac gttcacggcg gaaaatggaa tgctcacgac
      601 gatgggcaag ttaaagcgcg atgcgattgc cgcgcgatac gcggatgaaa tcgacaaagt
      661 gtacgcgaag aggactgcgt gaagagtttt cgcggcgagg cgctttcgtg gcaagtagtg
      721 gatggcgcta tcgagttggt gcttgatcgt gcgccttgca atgaaatcgg gtcagtcgcc
      781 ctggccgaac tggagaaatt tgttggcgcg ctgggcgtgc ttgagcgaga tgctcatgcg
      841 ctgattatta cgagtgcgcg caaggaaggc ttttgcgcgg gcgccgatct tcgggaactg
      901 tatcatcggt cgtcacaatt gccggctgcg gcgcggcttg aaggcgttcg cgattttcta
      961 gagcgaattc accgcgtgat gaatgcgatc gacgaggcgc cgttggttac gattgccgcg
     1021 gtgcatggcg tcacgtttgg gggtggattc gagctggcgc tggtttgcga tttgatcgta
     1081 gccgataaaa tggcgcgatt ttgttttccg gagttgcgtc tgggattgat tcccggtttc
     1141 ggcggcattc cgcgactgaa acgcgatctt ggcaatgcgc tggttcgcga tttgctcttc
     1201 acggggcgaa gcatcaatgc gacaaaggcg caagctgcgg gcctcgtgag ccaggttgtg
     1261 gcggagggag atgcgttgcg cgcggcgcgg gccacggcgg cgcagatcgg aaagtttgac
     1321 cgcgagacca cgaaggctgc gaagcggttt atcaagccca ttccccatga ggaattgcgg
     1381 cgggagattg agattttctg cgaattgttt acgaggccgg cagtagacgc gggcctgcgg
     1441 aaatttgtgg aaagcacgga tgcgctgccc tacttgccgt aggcaggcgt ggagaatgga
     1501 tggcgaacct tcaagagact acggaacaga ttcttgatct tgctgcggca cactttaaag
     1561 tgccgcggac gcagctgagc gccgacgacg atttcttcaa gaagcttggc attaacagcc
     1621 tccaggcgct tgatttgctc acgcgacttg aacaacactt tcgtattgag ctgcccgatt
     1681 acgaattgca ggacgtgagc gactttcgca cgctcgccga acgcatccag tcccgcctct
     1741 gatgctcttg ccacagggac acggagacac agagatggac atggatcggt taaaccatct
     1801 ctcgggaaaa attataggat gcgctatcga ggtgcatcgt gttcttgggc ctgggcttct
     1861 ggaagcgact tacgaagagg cgctttgcat tgagcttgcg gagtttggcc tgacgcatcg
     1921 gagacaatgg ccaattccaa tctcttacaa agggcgacga ctcgggaaat accgactgga
     1981 cttggtagta gaggatgcgg tcatcgtcga aattaagagt gtcgagagat ttgatccggt
     2041 gtttcaggcg caagttttga cgtatcttcg ggcagcggaa aagaagattg ggctacttat
     2101 aaatttcaac tcgcggctac tccgggacgg cataaagagg ataattcttt gagaatccat
     2161 ccctctgcat ctctgtgtct ctttggctaa ttcctgatgc ttgatctgaa taaatacaac
     2221 tgcttgggag aagcgctgcg cgcggcgatt gatcgctggc ctggggacat ttgcctgatt
     2281 gaatccgatc gggagcggga gaattgtcgg cccacgtatc gacaatttag cgaagcggcg
     2341 ctgccgctgg ctgcggcctt gcaggatgcg ggattgtcat ccggtacgcg cgttgcgatt
     2401 ttgatgacga atcagtctaa atggctgatt tcggcctacg ctgtgttcta ttgcggcggc
     2461 gttcttgtgc cgctggatta caagttacct gccgacgatc agttgaaatt gctggcgcac
     2521 tcgaaagcgg aatatctgat catagagttt cacctatggc gcgcgatcac aaaggctgag
     2581 aaattcggcg aactttcagc gcgaaccgtg cttgttaccg aagcgccggc aaacgcggag
     2641 ctggctggtg cacagcgttg ggaagagttt cgcgctgggc gtgaaccgga gtttgtgaag
     2701 cgcgaacgca aggatcccgc ttgcatcgtt tattcctcgg gcacgggtgg acgcccgaag
     2761 ggctgtgttt taacgcacga aaattatctg gaacaggcca ccgctgtggc gccgattttt
     2821 ccgttttggc ccggcgcgcg ctacctcagc atcatcccga cgaaccacgc catcgatttc
     2881 atgggcggct ttatcatgcc ttttaccggt ggtggcacag tggttcactt gcgcacgctg
     2941 aggccggaat acatccggga tgcgtttaca cgctacggga tcacgtacat ggcggtggtg
     3001 ccgctgatcc taaaaaatct tcagcgcggc ttaacagaga aattcgcggc gctacctccc
     3061 ttcaagcggc gagtgttaaa tctactggtt gcgacgaatc gtgcgttgac gaagagcaag
     3121 ccgcgcccga gcctcagccg caagctgctg aaagacattc acgccgcttt cggcggcggc
     3181 ctgcgtgcgt tgctagtggg aggtgcgttc acggaacccg cgaccataga attcttccat
     3241 gatctcggga ttccaattta taacggctac ggctgcactg aagcatgcac ggcgatcacc
     3301 gtgaacgatc agcggccatt tcggccggag acggtcgggg ggccggtgcg cggaatggaa
     3361 attcgcattc tgaattcggg cgaggacggc gtcggcgaag tggccgttcg cagcaaaacc
     3421 gtgatgtcgc attatctgga cgatcccgag caaacggcgg agacgattgt cgacggctgg
     3481 ctgattacgg gcgatctcgg tcgtctcgat gacttcggcc agttgcagct ctttggccgc
     3541 aagaaaaaca tgatcgtgac cgaagaaggg aagaacattt acgctgagga catcgaggcg
     3601 gttttcgacg gactggccgt gaaggagtct tgcgttttcg ccgcgaattt tctttggccc
     3661 gcgcgcacta tggtggccga gcgactcgtg ctcgttgtac acccggaagc gggtaagcag
     3721 gtgaatgccg aactggcgga ggaaatcgca gagcggaatc gccgcttggc caattacaag
     3781 cgcgtgggag gctacttggt ctgggagcgc gattttccgc tgactgcttc gctcaaatgc
     3841 aaacgtgggg aactggcggg gcagattcgc gaggccgtga gccgcgaaag cattgtgccg
     3901 ctgtagaatc gagcggctgt cgaatcacgg ataaatcatt tgaacactga gaagacgcgg
     3961 tttctagcca ttgtgaatcc cgccgcgggt ggaggtcgct gcggcgaact tgcgcctgcc
     4021 gcacttgagc gcgtgcggcg ggccctcgat gcgaaggacg ccacaatgga aattgtttac
     4081 acgaaaaagg ccggggaagc gacggaactg gctcgcgcag cataccgaga gcgtgtccgc
     4141 cacttcttgg ccgtcggtgg cgacggaacg tcctatgaaa tcatcaatgg cgtctttccc
     4201 gaatctctcg aaaacggacg ggtttctctg ggatttctac cgctgggcac aggaaattct
     4261 ttcttgcgcg acttcacgaa gaaaggcgtg gagtatacga tgcctgcgat tgcagagggg
     4321 cggtcacgcg tttgcgacgt gatccgcggc aggcatgcga gcggcgaaat atattttatc
     4381 aatgttctca atcttggatt tccggcggac gtggccgagc tgactaatcg ccgtttcaaa
     4441 tcgctcggcg agacaggata catttttgcg gtctttgccc ggctcgcgtg gctcttttac
     4501 ggaacgtttc cacaccgcat tgatgggcat gagtgggatc gacgcccgag tctttttctt
     4561 gctttcacga acagcaaatt caccggcggc aaaatgatga tcgcgccgaa ggcggattcg
     4621 gctgacggga aactggagta cgttcgttgg gcgccgatcg gccgcgcccg cctgctgtgt
     4681 ttgtttcccc ggcttttcac aggcacccac atcgaccatc ctctcgccac gcgcgcggca
     4741 gccgagcgcg ttgagtttga tctcgctgag cctgtaaata tcgtggtgga cggcgagaac
     4801 ctgaaactga acttgcaatc gctcgaaatt ctgccgggcg cgctggacgt gattgtatga
     4861 aaaaggcctg gctgactgtg cgctcgctga tactgtgggc ggtcagtgga gtgcattttg
     4921 gcgtcgtctg cacgtttctg gtatttctcg gaatcttcgt cgatccccgc aagaatgact
     4981 ggccgcagcg attctttttt cgaaatattc ttcggttcgc gggtgtgcgg tttgaagtgc
     5041 gccgcgctcc cggcttcgat ccgaaacgga cgagcatctt catctgcaat cacgtgaaca
     5101 ttttcgatcc cttcgtgatt tattccgcga ttccgcaatt cgtgcgcggc ttcgagctgg
     5161 aatcgcactt taaagttccg atctatggct ggatgatggg gcgctttgga aatattcccg
     5221 tgccggacac gcgcagccgc gaaggaatag aacgcatgac ccgcaaggca aaggaagcgc
     5281 tcgattccgg cgtcagccta atcgcgttcg ccgagggttc gcggacacgc catgggcacg
     5341 tgcagcgttt ccataagggc gtcttccggc ttgcacaacg ctttggcacg ccgatcgtgc
     5401 cgatgagcat cgtcggttcg tatcaatttt tccaaacggg caactggatg ctttatccgg
     5461 gcaagatcac agtctatctg cacgatacga ttgagacggc agacgtggag cgcgccgaca
     5521 tcgatcaatt agcagagcgc gtgcgaaaca ccgtcgcagg ccctgtggaa gaatcgctaa
     5581 agtgtgttta tactacccag caggcgagtg gtagcgtact gtccggatcg tgagccgggc
     5641 tggcgctggg gcgattgcaa actaatgtgt ggggtgggga ccttgcagcc tggcccttca
     5701 tacaaggcga gcctcagtgg gaatctgcgc gcgaggatgc cacatctctt ccaacgcaca
     5761 tgctgggtat gactgaaaac gggaagcaca accggcgttt tccgcggatc gcaacgccga
     5821 gtggcgtttg ggtcgcatgg cagcaggacg ggcagaaaca gtccgtctct cgggtctgcg
     5881 atctgaacat tggcggcctg tttattgcga cacccaaacc cgcatcggtt gggaccgtca
     5941 tttccgtctt gttttccgtc cctgaaggcg aaatccgagg tcatgccaca gttcgcaact
     6001 tcaaagccgg cgagggaatg ggcgtccagt ttacggccat gggtggacag gatacggacc
     6061 gactgcgaaa gctcgtcgcg cgtcttcttg atgcggcttc tagccagcaa gcgtgagcgt
     6121 gagcattcgt tgcgcatcag gcgcgattta tcccgtcaag gcgccgtcac gcccggaagg
     6181 gcgcgtccgg ctttcagttc gcgccacgta tatacgacac gatgaatcac ggtgagtgtc
     6241 gacaacgtgg cgatcaccca aagcgcttgc gccatgcggt tgaacagtcc gccgaggatc
     6301 agcaacacca gccgttccgg ccgttccagg aaccccactt tacaggaagg gattaacgac
     6361 tccgcccgag cacgcgaata gctcaccatg aaggagctcg ccatcgccac tgcggcaagc
     6421 gccatataga aaaagcgctg gatcagtgtg taatggacca cgaagcccag gtacagggcg
     6481 agatcggaat agcggtcgag cgtggaatcg aggaaagcgc cgaacgcagt cacgcgaccg
     6541 acgcggcgcg ccacttgacc atccgccata tccagaaagc cggcaaggaa gagaaccgcc
     6601 gcgccctggc gaaaatggcc cattgcgaag caggccgccg cccacgaatt cacggccaag
     6661 ccgagaaacg tgaacatgtt gggagtaagt cccgtggcgg cgagaccgct taccacatgg
     6721 cgcaggagcc aaccggcgcc ttctcctatc caataggtta taggccgttt tcgatcagaa
     6781 tgaggattcc ccatcggttt cgatgataac accgaaacgc aagcgattat gccgctcgca
     6841 gggtctagac ggaaattggc gaatgcgaag gagaggagga accccggccc gttctgcgcc
     6901 cggagaatct gtgatcgcaa cgcgcgcatc gatacggttg caccggcagg acccaggaaa
     6961 gtaagtgttc aacgattccg cgcttgtgcg aacgccgaac ctgggtgctt ccgcaactcg
     7021 ggcatgtatg agccgtcatc tttcacctgt ttggtttgtg gttccgcagc cgcgcctgtg
     7081 acccgatgct gcggatgcaa acgctctcgt caagcaagtg agcgttacgt aaggcattcg
     7141 accacacctg gcggaacggc ggaatcggtg aatcgctgta atggacgcag tacggcggcg
     7201 gatgcgatta cggggttttg cctgcatctg cttgtgatat ctgtgtctta cggttggcgg
     7261 gcggacggag ttgcacttgc acgttggttt tgctccagcg cacgcgaatt gcgtctactc
     7321 cttcttcgcc aagcccggtc gaaatcagtg gcgggccttg actgagatag ccggaggcag
     7381 ccggaacctc ccacgtttgc ctgagcgcgc cggaaatcat ttcgactcga acgcctatac
     7441 ccatcctgtt ctgggttgcg ccgcgaagcg cgaattgagc ccaattgtgc ttctcggcac
     7501 ctacggctct gagcaggaga ggcggccgat ggttttgcgt gatcaggaga tcgagagagc
     7561 cattaccgtc tgcatcgaag gcgatgacgc tgcgaggatc atgaagcgca atcttgtcga
     7621 ggccggtttc gggcgttacg tcgtgaaagc ccttcccgcc ttcgttgcgt aacagcgcga
     7681 tgcggccctc tcctgaaaac gtatcgccga cggcgacgag atcgatccat ccgtcattgt
     7741 catagtcaag cgtagcgatt ccccaagcgc gcatccatcc tggatcggga agcgcgaggc
     7801 gttgaaagcc cttgccggcg acatttcgcc agaggccgag ggtagtcggt tgcgaatggg
     7861 taagcgccac gtccatgtag ccgtccttgt tgaaatcctg cgctacggcg ccggccgttg
     7921 gcccctgggt ttctgctccc caccatgatg cgcttttgaa cgtgccgccg cgtccgttca
     7981 tcagcatcga aggcgtcggg cttaagccgg tgagaatgaa atcgatggca ccgtcgcctg
     8041 ttagctcggt ggcgattgcg cctgtgctgg atgcgctgcc agccaagtgt agtgcagatg
     8101 tcacgtcgga gaatgttcca tctccgttgt tgcgccacat gatgtttccg ggcgcgccat
     8161 cgtttggcca tacaaagggc tgtgacggat tcactagcgg agcattggaa agctgcgtcg
     8221 cgtatagatc gagatttccg tcgtgatcgt aatcaacaaa tgaaaggccc atcaccaggc
     8281 tgtccatttg aatgcccgat gcggccgtta catccgcgaa cgcgcctttt ccattattgt
     8341 gataaaggcg gacgccgcca ttgaagctga ttgccaggtc cggatggcca tcgttatcgt
     8401 aatcgccgac ggcgcaaccc atccccgcgc cctggaagtc gatttttgcg gcttttgtta
     8461 catccgcgaa tcgaccgcgg ccgaggttgc gatacagagc ggcgcccccg tttccgcctg
     8521 catcgactag aaagatatcg gggcgtccat cgccgtcgta atcgaagacg caagccccgc
     8581 tgccgaggaa gtccgccatc gaatgaacct cggatggtgc ggcaggcttc gcggttgatc
     8641 gggaaccgcc gcgtgcgccg cgtccgtggg cggcgacggt gctcgatgga aacaacaggc
     8701 ctgattggcg cgtcacgtca acgaaatgga ccgggacctc cggcgcgact gtatcgggtg
     8761 ggggaatctc agcggcccgc gaatatttgc cttgctcgcc atagtgatcg ccgaatgtgc
     8821 tgaggagagc gggatcgggc gcttgggtgg cgcttgcgtc cgctgtttgc cggcctgccg
     8881 cgtgacccct tttcgctaac gcttcagtgg cgcccgattc ggccgttgcg ttgaggggat
     8941 cgatcttcag agcggcctgg aatgccttga aggcggcatc gtattgctgt tcggccatgt
     9001 agctgaggcc gatgaggcat tgcgtttctg cgtcttcgcg atcgagcgac gcgactcttt
     9061 gaaaatcaac aagtgccgct tgtgtttgcc cggcggcgcg agcgagcagg cccaggttga
     9121 accacgcagt ggcgcttttg gggtcacggt tggccgaggt ggaaaggatt ctttgcgcct
     9181 gatcaaattg tcgcatcgcg agcagagcga ttccgctgtt gaggcagccg gtatcggaat
     9241 tgggcaccat gatgcaggct gtttggaatc gcgaaaacgc ctcgctaaag cggtgggcgt
     9301 ccatgagggc aacgccgacg ttattgtctc gaattccgcc agcaaaaact gagctttggt
     9361 ccgccgccgc gtcttccggc ggtgacgtcg cgggctgcgg ccggccacgc ttggtacttg
     9421 atgtattttc cctgggatga tttttcgatg gccgggcctg ctttgcgccg actgcgtacg
     9481 cggtggcaaa aagcgcgata caagcgagaa gggaagcggc gcgccaaagc gtggccctcg
     9541 gatgtgtgcg cgcgattcgc acgtggtgaa aaacctcttt tcactgtcga ttagaaacgg
     9601 tgcccgtgtt gcagctaagg cacttaagcc cgattttaat cgcgggactg tcgattagaa
     9661 atcggtgcag ttggcaatta tagggcgatg atttatgggg ttcgcaagat ctcgaaatgc
     9721 gatcaattgt cacgcccata ttttatcagg ctgcacaact tgacaggcgt gcatgccatg
     9781 atgcaacgca ttgctaggcg ggcggttaga attttgaggt ggctttgttc cgatggacgc
     9841 catagctcaa aaaaatccga atgctgcgag cggaaggcta tccaaagttc gtggcgttca
     9901 gcaagggcta cgctgagcct cggcctctgt gccgtgcggc aaggccgcgg cgccacattt
     9961 gagtattgaa ccgctactcg taacggaggg cctcgatggg atcgagagag gccgccttac
    10021 gggcgggata atagccaaaa atcacgccga ttccggcagc gaatagcaca gcggcaccga
    10081 tggccagcga agagattgcc gtcggccagt tcaaaagctg cgaaacgcct gccgacattc
    10141 ctataccgaa aatgattcca atgaagccgc cgatgagact caacgccagc gcctcgataa
    10201 ggaattgagt gcgtacgtcg tgctcggtgg caccgatggc catacgtacg ccgatctcgc
    10261 gcgttctctc cgtgacggaa accagcatga tgttcataat gccgatgccg cccacaagca
    10321 gcgataccga ggccacgctg ccgagcatca tggtgaaggt ctcgctggtc ttttctctcg
    10381 ccgccgcaat atccgcagga tggcggatat
//
LOCUS       DATNDT010000271        11901 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2993538, whole genome shotgun sequence.
ACCESSION   DATNDT010000271 DATNDT010000000
VERSION     DATNDT010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11901)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11901)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11901
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2993538"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..241
                     /locus_tag="VN788_08775"
     CDS             <1..241
                     /locus_tag="VN788_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20661.1"
                     /translation="NRGVHMAPAAMHLALPRFAQGGLVSKDSANAGATHTLQVGLEEG
                     LVVKHLRSKGAGRAIVEQLANNPKTANRALGRAKD"
     gene            complement(267..554)
                     /locus_tag="VN788_08780"
     CDS             complement(267..554)
                     /locus_tag="VN788_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010211121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU20662.1"
                     /translation="MSPEARAASEAATKKMLREMELRDIRKARKITQAKLAKRLKIGQ
                     DAVSKIERRADMYFSTLADVIHAAGAQLQVIARFPDGESYLVKMGKGRKRK"
     gene            complement(580..936)
                     /locus_tag="VN788_08785"
     CDS             complement(580..936)
                     /locus_tag="VN788_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016783068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HXU20663.1"
                     /translation="MEWLVEYTDEFGAWWDGLTDMEQRSVDSSVGLLRKSGPALGRPH
                     ADTVKGSRHANMKELRIQHGGRPYRVLYAFDPRRCAILLLGGEKTGKARWYREFVPKA
                     DAIYDEHLRALGETDG"
     gene            946..1074
                     /locus_tag="VN788_08790"
     CDS             946..1074
                     /locus_tag="VN788_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20664.1"
                     /translation="MPLMAYERQWYQGPIRLTAATKARIMAIAPRVAAVQRLQNAC"
     gene            1225..1485
                     /locus_tag="VN788_08795"
     CDS             1225..1485
                     /locus_tag="VN788_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20665.1"
                     /translation="MSSKSSITAGIPARKDANPLREPNPEMRAEAFWRGAAEARERRG
                     ETTTAIFFRNLAWQAAQERAEFGRQMQAALAAGRTALEGGRP"
     gene            1509..1679
                     /locus_tag="VN788_08800"
     CDS             1509..1679
                     /locus_tag="VN788_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20666.1"
                     /translation="MTANKIREGCKAGYGSPVEGGMENAQVYFLAEIAAQLAELREEI
                     AEFRKQFLNRSR"
     gene            1831..2403
                     /locus_tag="VN788_08805"
     CDS             1831..2403
                     /locus_tag="VN788_08805"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU20667.1"
                     /translation="MKSGEEAVADQPLLYSLSAAAVKLSIGARTLSKLIRDRAIRPKR
                     VGARTLIPGRELERFMAARAEAESRKRAEREERRRMALQIRQTTRRLSRQEWDRTRQS
                     LWAERSRLRAGVAAALLAGLSEERIAPETFDQSMRQLFAGGYESYAAEAMELREVLLP
                     GAAFPPLLAALDTLIDFLQGADLRLPACPR"
     gene            2446..2679
                     /locus_tag="VN788_08810"
     CDS             2446..2679
                     /locus_tag="VN788_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20668.1"
                     /translation="MTGYFLIEILGAKQLIRVDGLRDPTHAFDLETRFGREQTFKTCF
                     IAGDDELLVFTSEELETSTDVFRRRPDHEMRTL"
     gene            2738..3043
                     /locus_tag="VN788_08815"
     CDS             2738..3043
                     /locus_tag="VN788_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20669.1"
                     /translation="MTQFGKNAKLWSSKFARFVEGYGAERLAAKLQMHPSAVYHWIRG
                     ATSPRPVHAAIIQGLARESGVRLSFEDIYHHRSHSRDLRAAKEAHQSKAPAAALSIN"
     gene            3116..3406
                     /locus_tag="VN788_08820"
     CDS             3116..3406
                     /locus_tag="VN788_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20670.1"
                     /translation="MPKTKKASTGNASLPRPCEIRARTMEKMVSLPPPILRQITQMDL
                     AESDMLRREISRLMSAWCKKKREIQRALEAGATIEEGTRTVEVRQRRRLVIL"
     gene            complement(3808..3883)
                     /locus_tag="VN788_08825"
     tRNA            complement(3808..3883)
                     /locus_tag="VN788_08825"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3848..3850),aa:Ala,seq:tgc)
     gene            4070..4782
                     /locus_tag="VN788_08830"
                     /pseudo
     CDS             4070..4782
                     /locus_tag="VN788_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611550.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4766..5782
                     /locus_tag="VN788_08835"
     CDS             4766..5782
                     /locus_tag="VN788_08835"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HXU20671.1"
                     /translation="MNAWRSNHVFSPVLVIATPYPLLLAIPVAVIGAVSLSAKRGIII
                     KNPAALEQIDSCRTFMFDKTGTLTYGKPSLTGIVCAQGVAETEIPAESASLERYSKHP
                     LAKAIVDAAQQRGYVLRSVAHVSERPGAGLRGALGGSEIFITGRNKLSSMASNLPPQE
                     PGLECLVFRNGAFAALFGFEDLPRADSQLFIDHLSPRHRVAKLILASGDREPEVRALA
                     AKVGIKTALGARSPEEKVELVRSETHRGKTVFIGDGINDAPAMQAVGIAFGHENEITS
                     EAADAVVLNQSFEKVDELIHIGRRMRRIALESAVGGMVLSGARMLFAACGLLSPLQEP
                     LHRS"
     gene            6074..7117
                     /locus_tag="VN788_08840"
     CDS             6074..7117
                     /locus_tag="VN788_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011556727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXU20672.1"
                     /translation="MKALVFRGPNQIGIEQVPIPTPGVGEAVIRVTLTTICGTDLHIL
                     KGEYPVRPGLVIGHEPVGVIHELGVCVTGYEVGERVLVGAITPCGQCDYCLGGDLSQC
                     GGPIGGWKFGNTINGAQAEYLLVPNAQANLAKIPDDLGDDQVLLLADIASTGISAAES
                     GKVKIGDTVAVFAQGPIGLCATAGAKLKGAGLIIAVDSDHVRTAMAKRMGANIVVDHT
                     KCDVVEEIRHLTNGKGVDVAIEALGTQATFESALRVLRAGGTLSSLGVYSGKLSVPLE
                     PFAAGLGNHTIVTTLCPGGKERMHRLMELVRCGRLDLRPLITHTFSLDKITEAYALFA
                     QHRGGAIKVAIRP"
     gene            7245..8144
                     /locus_tag="VN788_08845"
     CDS             7245..8144
                     /locus_tag="VN788_08845"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXU20673.1"
                     /translation="MKAAQAGSRVALKNILFLTDFSEPSERALPFALSIAREFGARLH
                     AVHVMIPQPYTYTTPELMDLAIKAQEECAESSMQEVDAQMAGLVHKTHIVRGTGLWLP
                     LVEVMNDCSADMLVLGTHGRTGPQKLLLGSVAEEILRLSAVPVLTIGPEVRSSTHTAA
                     RFRRVLFATDFSDESRAAKPYAISLARENQAELVLLHVIPQRKGHGERLPAEISVANA
                     MHELYEMVPPGAELWCRPEPTVRYGEPAPTILATAEELGADLIVLGVRSGAGRLGAAT
                     HLERAVAHKVVSHAKCPVLTVRG"
     gene            8203..10974
                     /locus_tag="VN788_08850"
     CDS             8203..10974
                     /locus_tag="VN788_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015172249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HXU20674.1"
                     /translation="MNDQRIAAQTPHQSPGQPNDPSHDFLRSRSAPLDSIFRPQNVAV
                     IGATDRDGSVGRSVLVNLRGASYRGEVFAVNPKRTQLLDEPAFASIGKVPQTIDLAII
                     VTPAATVPSLVRECVQARVKSAVVISAGFRERGPEGAELERQVARELAGSTLRLIGPN
                     CLGVMNPLIGLNATFAQTIARPGSVAFLSQSGALLTAILDWSLREQVGFSSFVSTGSM
                     LDVNWGDLITYLGDDPHTKSIVIYMESVGDARSFLSAAREVTLSKPVIVLKAGRSEAA
                     SKAAASHTGALTGSDAVLDAAFRRCGVLRVNSISDLFYMADVLGKQPRPRGPRLTILT
                     NAGGPAVLATDTLIAEAGELAVLPGESLQSLDAFLPPHWSHGNPIDILGDADSDRYAK
                     AASIAAPNPESDGLLVILAPQGMTDPTEVARRLDPHARSHQKPILASWMGGGHVSHGK
                     SILSAAGIPTFDFPDTAARMFAYLWRYTYNLRGLYETPTLTGELPPRSRERTAVLLKE
                     IRAQGRTLLTEYEAKQILSLCGIPTVETHLALTPEEAAVFANQIGFPVVLKLHSATIT
                     HKTDVGGVQLNLADGPAVKAAFKEISDSVSAKAGPGNFLGVTVQPMVRRQGYELLIGS
                     TTDAQFGPVLVFGTGGQLVEVYRDRSLGLPPLNATLAQRMMEQTRIYTALKGIRGRAS
                     IKMDELEGILVRFSLLVAQQRWISEIDVNPLLASPDGFLALDARIVLHGHTTQAAKIP
                     KLAIRPFPGHYESRWEMKDGTDVTIRPIRPEDEPLMVKFHQQLSDRSVYLRYFQVNAL
                     DRRVAHERLLRICFIDYDREMALVAQRGTGDAAEILAVGRLMKAHTDNEAEAAILVRD
                     QFQRRGLGSELLKRLIDVARREGIGRILLNMLRENFEMQAMAKKLGFRLEDSPDQTLV
                     FGVRDLSGA"
     gene            complement(11697..>11901)
                     /locus_tag="VN788_08855"
     CDS             complement(11697..>11901)
                     /locus_tag="VN788_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXU20675.1"
                     /translation="GTFRAECLNAHWFISLTEAQQIVETWRWEYNESRPHRAFGEKTP
                     KEFAHEIAASRDLITSQTAENSP"
BASE COUNT         2473 a         3513 c         3514 g         2401 t
ORIGIN      
        1 caatcgcggc gttcacatgg ctcccgccgc gatgcacctc gcgctgccgc gcttcgccca
       61 gggcggcctg gtgagcaagg attccgccaa cgccggcgcg acgcacacgc tgcaggttgg
      121 ccttgaggag gggctcgtgg tgaaacatct ccgcagcaag ggcgccggtc gcgcaattgt
      181 cgagcagttg gcaaataacc cgaaaacggc gaacagggca ctggggcggg ccaaggactg
      241 agcgtccggc aagacaccgc gcggcgctac ttgcgcttcc tccccttgcc catcttgacg
      301 agatacgact cgccgtcggg gaagcgcgcg atcacctgta gctgcgcccc ggccgcatgg
      361 atcacgtccg cgagcgtgct gaaatacatg tcggcgcgcc gctcgatttt cgagacggcg
      421 tcctgcccga ttttcagccg cttcgccagc ttggcttgcg tgattttccg cgccttgcgg
      481 atatcgcgca gttccatctc tcgcagcatc ttcttggtcg ccgcctcgga ggcggctcgc
      541 gcttccgggc tcatttttgc ccgcaactcg ctatatggcc tagccatcgg tttcccccaa
      601 agccctcaaa tgctcatcgt aaatcgcatc cgccttcggg acgaattcgc ggtaccaccg
      661 cgccttgccc gtcttttccc cgcccagcag cagaatcgcg caccggcgcg ggtcaaaggc
      721 atagagcacg cgataggggc gtccgccgtg ctgaatccgc agttccttca tgttcgcgtg
      781 gcgcgaaccc ttcaccgtat cggcgtgcgg ccttccaagc gccgggccgc tcttgcgcag
      841 aaggccgacc gaactatcca cgctgcgctg ctccatatcc gtcaggccgt cccaccaggc
      901 cccgaactca tcggtgtatt ctaccagcca ttccatgcga ttactatgcc tctgatggca
      961 tatgagcgcc aatggtacca aggtcctatc cgcctcacgg cggcgacaaa ggcgcggatc
     1021 atggccatcg cgccgcgcgt cgccgccgtg cagcggttgc aaaacgcctg ctaaacgtcc
     1081 gctaaccacg aattccgcgc gccggtacga aagacctatt gcgccctgga tggggcagag
     1141 gtaccgtaaa agggtagtaa gcggggcacc gcggtgcgtt caacacctca gcgcccctaa
     1201 aactcaaaag cgaggtgagt cgccatgagc tctaagtcga gtataaccgc aggaattccc
     1261 gcccgaaaag acgcaaaccc gcttcgcgag cccaatccag aaatgcgcgc ggaggcattc
     1321 tggcgcggtg cggccgaggc ccgcgagcgc cgcggcgaga cgaccaccgc catcttcttc
     1381 cgcaatctgg cctggcaagc cgcacaggag cgcgccgaat tcggccgcca aatgcaggcc
     1441 gccctcgccg ccggccgcac cgccctggaa ggaggccgcc cgtgagtcaa atcaatgagc
     1501 ggaagccaat gacagcgaac aaaattcggg agggctgcaa ggcgggctac gggagtccgg
     1561 tagagggcgg catggaaaat gcgcaggttt actttttggc tgagattgcg gcgcagcttg
     1621 cggagctccg cgaggaaatc gcggagttcc gcaaacaatt cctcaatagg agccgatgat
     1681 gggcggcctt ttcctgcttc tcatagccct cgccgtttcg gtctatggac ttgcggcgct
     1741 ccgctcggcc ctggcgtcgc cggaattcaa gcgggacctg cggcggctcc gcgaaatccg
     1801 ccggcaccgc cgaattgcga ggaggtgtgc atgaaatccg gtgaggaagc ggtcgcggac
     1861 cagccgctct tgtactcgct ctccgccgca gccgtaaagc tctcgatagg cgcgcggacg
     1921 ctcagcaagc tcattcggga ccgggcgatc cgtcccaagc gcgtaggcgc ccgcacgctg
     1981 atcccaggca gagagctcga gcggttcatg gccgcccgcg cggaggccga aagccgcaaa
     2041 cgcgccgagc gtgaggaacg ccgccggatg gcgctccaga tccggcaaac aacccgccgg
     2101 ctctcgcggc aagaatggga ccggacgcgg caatcgcttt gggcggaacg atcgaggctc
     2161 cgcgccggcg tcgctgctgc cctgctcgcg ggactgagcg aggagcgcat cgctcccgaa
     2221 acgttcgatc aatccatgcg ccagcttttt gccggcggtt atgagtccta cgcggcggag
     2281 gcgatggaac tgcgcgaggt tctcttgccg ggagccgctt tcccgccgct gttagccgcg
     2341 ctcgacacgc tgattgattt cctgcaaggc gcggacctcc gcctgccggc gtgcccgcga
     2401 tgaaccacgg cgagccgaat catccagacc caaggaggag aaaagatgac gggctatttt
     2461 ttgattgaga ttttgggggc gaagcaattg atcagggtcg atgggctccg cgatcccacc
     2521 cacgcgtttg accttgagac gcgcttcggt cgggagcaga cgtttaagac ctgcttcatc
     2581 gcgggcgacg acgaactcct cgtttttacg tcggaggaac tggaaacgag cacagacgtt
     2641 tttcgccggc gtcccgacca cgagatgcgc acgctgtgat agattcgggc ggtcccgcgc
     2701 ggcgttcctc gcgtcgcgcc tgatggaggg cggcccgatg acccaattcg gcaagaatgc
     2761 gaagctttgg agctcgaaat tcgcccgttt cgtggaaggc tacggcgcgg agcggctcgc
     2821 cgcgaagctg cagatgcatc cgtctgcggt ctaccactgg attcgcggcg cgacgtcgcc
     2881 acgtccggtc cacgccgcca ttattcaggg gctcgcgcgc gagagcggcg tccggctcag
     2941 cttcgaggac atctaccacc atcggagcca ttcccgcgac cttcgcgccg cgaaggaagc
     3001 gcaccaatcc aaggctccgg ccgccgcgtt atcgatcaac tgagcgcgtc ccccggcagt
     3061 gccggggttc ccggcacgaa gcaaaccgta tagcagtcga acgcgaggtg tcgccatgcc
     3121 gaaaacgaag aaagcgtcga cggggaacgc ctcgctgcca aggccgtgcg agatccgcgc
     3181 acgcacgatg gaaaagatgg tatcgctccc gccgcccatc ctcaggcaga tcacgcaaat
     3241 ggacctggcg gagtccgata tgctgcgccg cgaaatctcg cggctgatgt cggcatggtg
     3301 caaaaagaag cgggaaatac agcgggctct ggaggcggga gccacgatcg aggaagggac
     3361 acgaacggtt gaggttcgtc agcgccgccg actggtgatc ctatgacgga caaaaatgcc
     3421 gtgaaatggc gatgcggcgc cgccctcatc gccatcgaga ccgaatggtc agaaaatcgt
     3481 ggaattctcc gcgccgccgg gatacatcac gcaaaaggag ctagtcgaac tggccgtggc
     3541 gaagctgcag gcgaaggaaa tgaataccaa ggtccgcgaa ctgcgagctc aattcgccgc
     3601 ctggagcaag gattcgaggt cgaggcgggc gtgcgatcag cccgaaccga acagcggaag
     3661 gtcctactca tcgactgacc gccgctctcg ccgaaacggg gtccctcctt gccttttgac
     3721 gactcaccgg aagcccgtgg accgcgttta tttctgccaa ccccgcatga cactggcatg
     3781 acactggcgc ggcggacctc aaaaaagtgg tggacgtggc cgggatcgaa ccggcgaccc
     3841 cctgcttgca aagcaggtgc tctcccagct gagctacacg cccacgcggt gcaaccaata
     3901 cactttcaac agtttacagc gagttcttca actagtccaa cttcgagttc cggtccattc
     3961 agtccaacaa cggctttttc agaagggagc ttgaccggcc ccggtatttt tgacccacct
     4021 gaagggttag tttccggcaa aatctacgtc tgaaaaagga ggatttgccg tgaagcagaa
     4081 gcgattcaac gtcgaacaga ttgtggcggt gttaaagcaa gcagaagcgg gagtactgct
     4141 cgcagagttg atccggcggg ttgtaatttc cgaacagacc ttctatcgat ggaagaagca
     4201 atacgtcgga ttggaggtcg accaggttcg tcagctcaag cagcttcagg aagaagacac
     4261 ccggttgaag caattggtcg ccgatctgac gctcgacaag gccatgttgc aggatgttct
     4321 aacaaacaaa ttctagagcc ctcgcggcgc cgtcaggtag tcgactacct gcatggcagc
     4381 taccgggtga gggagcggcg tgcatgcagg gcagcccggt tgaatcgcgg gacattccga
     4441 tatctgggtc acaaagatcc acgaaccgct cttcggatgc gcatccgtga gatcgcccca
     4501 acgagagtgc gatacggata tcggaagatc cgcgtgctac taaaccgcga gggctggaaa
     4561 gtaggcaaat acttggtaga acgcctgtat cgggaagagg gattaacgct gcaccagcgg
     4621 cggaaacgcc ggcatcgagt agctgagcat cgctgtgaac gcttccaccc aacgggaccg
     4681 aatcaggtat ggtcgatgga ttttgtggca gatcaattag cggacggaag aaggttccgt
     4741 tcactgaccg tggtggacat ctatagtgaa tgcttggcga tcaaatcacg ttttctcgcc
     4801 cgttcttgtt atcgccacac cgtatccgtt gcttctggcc atacctgtcg cggtcatagg
     4861 agcagtttct ctatccgcta aacgaggcat catcatcaag aatccagcgg cactcgagca
     4921 gatcgactct tgccggacct ttatgttcga taagactggc acacttacat atggcaagcc
     4981 gtccttgact ggaattgtgt gcgctcaagg cgtcgctgaa accgaaatac ctgctgagtc
     5041 cgcgagtctc gaacgctatt ctaagcatcc tcttgcaaaa gcaatcgtgg acgccgcaca
     5101 acagcgtgga tacgtcctac ggtcggttgc ccatgtcagc gagcgcccgg gagcagggtt
     5161 gcgcggcgcg ctcggtggca gcgagatctt cataacgggc agaaacaagc tctcgtccat
     5221 ggcatcgaat ctgccgccgc aggagcccgg cctcgagtgt ttggtatttc ggaatggagc
     5281 ttttgcggca ctttttggtt ttgaagattt gcctcgcgct gacagccagc ttttcatcga
     5341 tcatctgtcg ccgcgtcacc gcgtcgccaa attgattctc gcatcgggcg accgcgaacc
     5401 tgaggtccgt gcgttggcgg cgaaggttgg gatcaagacg gccttgggag caagaagtcc
     5461 agaggaaaag gtggaattag tccgttcaga aacacaccgg gggaagacag tatttattgg
     5521 cgacggcatc aacgatgctc cagcgatgca ggcagtgggt atcgcattcg gccatgagaa
     5581 tgagatcact tcggaggccg cagacgccgt ggtcttgaac caatccttcg aaaaagtcga
     5641 cgaattgatc cacattggca ggcgtatgcg caggatcgct cttgaaagcg ctgttggcgg
     5701 aatggtgcta agcggtgcga ggatgctctt cgctgcctgc ggtttgcttt ccccattgca
     5761 ggagccgttg cacaggagct gatcgacctg gctgcggtgc tcaacgccgt ccgagtggcg
     5821 ctccctttcg aaaagttgag ggatttctag gcgactagtc ggccgagctt gcccgtctca
     5881 tcggcaaggg agagtctacg cgcgcgaacc ggagtgcgcg acgcggccac tgtgacgtcg
     5941 gtcacatccg ccagtgatgt tggtcatggc ctctttcttt tctttcttgc ataaatagtc
     6001 gtggccagga gagaggagaa cgagcatggc aacactagaa tcagtttcat taagcggcgc
     6061 gggcaccacc accatgaagg cattggtctt ccgtggccca aaccagattg gcatcgagca
     6121 ggttcccatc ccgacgcctg gcgtagggga agccgtcata cgcgtaactc tcaccacgat
     6181 ctgcggtacc gacttgcaca ttcttaaagg agaatacccg gtcaggcccg gtctcgtgat
     6241 cggccacgaa cctgttggtg tcattcacga actcggcgtc tgtgtcaccg gatacgaagt
     6301 tggagagcgc gttttggtcg gagcgattac gccgtgcggc cagtgcgact actgtttggg
     6361 tggagacttg tcccagtgcg gagggccgat cggcggttgg aaattcggaa atacaatcaa
     6421 cggtgcccag gcagaatacc ttcttgttcc aaatgcgcaa gccaacctcg caaaaattcc
     6481 ggacgacttg ggcgatgacc aagtactcct cttggccgac atcgcatcca ccgggatttc
     6541 cgcggcggaa agcggaaagg tgaaaattgg tgacactgtc gcagtcttcg cccagggacc
     6601 gatcggcctc tgcgcgacgg cgggcgcaaa gctcaaaggc gcgggtctga tcattgctgt
     6661 cgattcagat cacgtgcgca ctgcgatggc gaagcgcatg ggcgccaaca ttgttgtcga
     6721 ccacacaaaa tgtgacgtcg ttgaggaaat tcgtcacttg accaatggca agggagtaga
     6781 cgtcgctatc gaagcgctcg gcacccaggc cactttcgaa agcgcacttc gtgtgctgcg
     6841 agctggtggc actctgtcga gcctcggtgt ttactccggc aaactgtccg taccgctgga
     6901 accgtttgcc gccggtcttg ggaatcacac gatcgtcacg actctttgcc cgggcggcaa
     6961 ggagcgcatg catcgcctca tggagttggt ccggtgtggc cggctggact tgcggccgct
     7021 gatcactcac acgttttcgc tcgacaagat caccgaagct tatgccctct tcgcacaaca
     7081 cagaggcgga gcgatcaagg tcgccatccg gccataggat ctcgcagaaa ggctggttct
     7141 ggagaagttt cagagccgat cggtgatgcg gcgcatctag gccggtgaca ctcgtcaccg
     7201 tggtgcgtat acttttcgtt cataagtagt tttagaggtg gattatgaaa gctgctcaag
     7261 cagggagtcg ggtcgctctt aaaaatattc tcttcctcac ggatttttca gaaccatcgg
     7321 aacgcgccct acctttcgca ttgtcgatag cacgagaatt cggggcgaga ttgcacgcag
     7381 tgcacgttat gattccacag ccgtatacgt atacgactcc ggagctcatg gacttagcca
     7441 tcaaggcgca agaagagtgt gccgagagtt ctatgcaaga ggtggacgct cagatggcgg
     7501 gtcttgtgca taaaacccac atcgttcgcg ggaccggcct ttggctgcca ctcgtggaag
     7561 ttatgaacga ctgttcggcg gacatgcttg tgctgggcac gcacggccgc acgggtccac
     7621 aaaagcttct gctcggttcc gtggcagagg aaatcctccg cctgtccgcg gtgcccgtgc
     7681 tcacgatcgg tcccgaggtg cgttcaagca cgcatactgc ggcacgcttc cgccgcgtgc
     7741 tctttgctac ggattttagc gatgaatcca gggcagcgaa gccatacgcg atttctctcg
     7801 cacgggagaa tcaggccgag ttggtcctgc ttcatgtgat tccgcagaga aaggggcatg
     7861 gagagcgcct cccggctgaa atctccgtgg caaatgccat gcacgaatta tacgaaatgg
     7921 ttccgccggg agccgaattg tggtgccgcc ccgagccaac tgtcagatac ggtgagcccg
     7981 ctcccacgat cttggcaact gccgaggagc tgggcgccga tttgatcgtt ctcggtgttc
     8041 gaagtggggc aggacgattg ggagcggcga cccatctcga gcgagccgtt gcccacaaag
     8101 tggtgtcgca cgcgaagtgc cctgtactca ctgtgcgtgg ttgatcacgt ggattttggt
     8161 gggcctcttg aaagagggcg gcaatctgcc gatagtattg tcgtgaacga ccagcggatt
     8221 gccgcccaaa ctcctcatca atcgcctggt caaccaaacg acccgtcgca cgatttccta
     8281 cggtcgcgtt cggccccact cgattcgatt tttcgccccc aaaacgttgc ggtgatcggt
     8341 gctacggacc gcgacggcag cgtcgggcgt tccgttctcg tgaatctccg tggtgcctct
     8401 taccgcggcg aggtgtttgc cgtaaatccc aagcgcaccc aattgttgga tgagccggca
     8461 tttgcctcca ttggaaaggt tccgcagaca attgacctcg ccattattgt cactcctgct
     8521 gccaccgttc ccagcttagt ccgcgaatgc gttcaggcca gggtgaagtc ggcagtcgtg
     8581 atttctgctg gctttcgcga gcgtggtccg gaaggcgcgg agttggaacg ccaagtcgcc
     8641 agagagctag ctggcagtac actgcgtctc atcgggccga attgccttgg cgtgatgaat
     8701 ccgctcatcg gtcttaacgc gactttcgcc caaacgatcg cacgccctgg cagcgtcgct
     8761 tttctcagcc aaagcggtgc cttgctcact gcgatcctcg attggagtct tcgagagcaa
     8821 gtgggattca gctcttttgt ttcgaccggc tcgatgcttg acgtcaattg gggagatcta
     8881 atcacttatt tgggcgatga cccgcacacc aaaagcattg tcatctatat ggaatcggtg
     8941 ggcgatgcgc gatcgtttct ttccgcggcg cgtgaagtaa cgctaagcaa gccagtcatc
     9001 gtgctcaagg caggccgttc agaggctgcc tcaaaagccg cggcttctca cacaggggct
     9061 ttgacaggca gcgacgccgt gctcgacgct gccttccgcc gatgcggggt tctgcgcgtc
     9121 aacagcatct ccgatctctt ttacatggca gatgtgctgg gcaagcagcc ccgcccccgc
     9181 ggcccgcgtc tcacaatcct taccaacgca ggcggtccgg ctgttcttgc aacagacacc
     9241 ttgattgcgg aagctggcga acttgctgtg ctgcctggcg aatcgctgca atccctcgac
     9301 gcgttcctcc ctccccattg gagccacgga aatcccatcg acattctcgg cgatgctgat
     9361 tcggatcgtt acgcgaaggc cgcatccatc gcagcgccaa atcccgaaag cgatggtttg
     9421 ctcgtgatcc tcgccccaca gggaatgacg gatccgaccg aagttgcgcg gcgcctcgat
     9481 ccacacgcgc gttcacacca gaaacctatc cttgcaagct ggatgggagg cggacacgtc
     9541 tcgcatggca aatcaatcct cagcgcggcg ggcattccga cgtttgattt tccagacacc
     9601 gccgctcgta tgttcgccta cctgtggcgg tacacgtata acctgcgcgg tctgtacgaa
     9661 acgccgacgc tcacaggaga attaccgccc aggtcgcgcg agcgaaccgc cgtactactc
     9721 aaagaaattc gagcccaggg aaggactctg ctcaccgagt acgaggcgaa gcagatcctc
     9781 tcgctgtgcg gaattcccac tgtcgagacg cacctggcac tcactcctga agaggccgcc
     9841 gttttcgcga atcaaattgg gtttcccgta gttttgaagt tgcactccgc aacgatcacg
     9901 cacaagacgg atgtcggtgg cgttcaattg aatctggcag atggccctgc ggtgaaagcg
     9961 gcgttcaaag aaatttcgga ttccgtttct gcgaaagcgg gccctggaaa ctttcttggc
    10021 gttacggtgc aaccgatggt gcgccgccaa ggatatgaac tgctcatcgg cagcaccacg
    10081 gacgcgcaat tcggtcccgt tctcgtcttc ggaacgggcg gccagctcgt cgaggtctat
    10141 agggaccggt ctcttggttt gcctcctctc aacgccacgc ttgcgcaacg catgatggaa
    10201 cagacgcgca tctacacggc gctgaagggc attcgcggcc gcgcttcgat aaaaatggat
    10261 gaactcgaag gaattctcgt ccgcttcagc cttctggttg cacagcaacg atggatcagc
    10321 gagattgacg tgaatccgtt gttagcaagc cctgatgggt tcttggcgct tgatgcgcgg
    10381 attgttctcc acggacacac aactcaggcc gctaaaattc ccaagctcgc cattaggccg
    10441 tttcctggcc actacgaatc ccgatgggaa atgaaagatg gaaccgacgt gacgattcgc
    10501 ccgatccgtc ccgaagatga accattaatg gtgaagttcc atcaacagct ttcggatcgc
    10561 agcgtctact tacgttattt ccaggtcaac gcgctcgatc ggcgcgtagc ccacgaaagg
    10621 ctcctgcgca tctgcttcat cgattacgac cgcgagatgg ctctcgtcgc gcaacgcggc
    10681 acgggtgacg ctgctgagat actggcggta gggcgcctca tgaaagcgca tacggacaac
    10741 gaagcggagg cggcaattct cgtgcgtgac caatttcaac gccgtggcct cggttcagaa
    10801 ctcttgaagc gcctaattga cgtagctcgc cgggaaggca tcggacgcat ccttcttaat
    10861 atgctccgcg agaacttcga aatgcaggcg atggcaaaaa agctaggatt ccgtctggaa
    10921 gattcccccg accagacgct tgtcttcggc gttcgggatc tttcgggagc atagtgcact
    10981 ccgcacaagc tactttgcgt cgccgctctc cggttgccga gcgccggacc ccaaagacgc
    11041 atagcgcgat cgctctcgca cagcgcccga ggttcgcgac gcacatcgtt tcgatccgcg
    11101 caccagcacg aggtgcgacg gatgcagcga aagagatggt gcctggctaa ggccggcttg
    11161 cggggtcgcg gtgcattcac gaagcgatcg tgacgatgcc gccgattata ccgagaacgc
    11221 ctccaagaat tcctccagct ccgaacacga aatcgcttgt tcggattcgt gtggaaccct
    11281 tggtgcaaaa ggggcaacgg cccgttcaga tctcctcggc tccatactgg caggcccgtt
    11341 ccaagtctcc cggcacgttg tacgaaggcc aatatcgccg tgtggaaacc gagggagagt
    11401 gcccgcacac acaagctgcc gtcatcgtgg acggccgtgt gccgatgaga gttgcaagac
    11461 gcaccggcaa ttctctcatt actatatttc accacaggag cgggaacagc gcagaaaagg
    11521 cttgcgcttg cagttcgagt tcagaagaag tcgcgttctc gcatcttgca ggccatccaa
    11581 cgagagcttc cttccgcatt ggttcgtgga gatttcgatt gaccggaccg actttttgta
    11641 ccaagggacg tgagagagtg tacatcactg atcggaccgg cttttctgta ccatctctag
    11701 ggcgagtttt cggctgtttg tgacgtaatg agatcgcggc tagccgcgat ctcatgggca
    11761 aattctttgg gtgtcttctc cccgaaagcc ctgtgagggc gactctcatt gtattcccag
    11821 cgccaagtct ctacgatctg ctgggcttcg gtcaatgata tgaaccaatg cgcgttcaga
    11881 cattctgccc gaaacgttcc g
//
LOCUS       DATNDT010000272         2732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_696515, whole genome shotgun sequence.
ACCESSION   DATNDT010000272 DATNDT010000000
VERSION     DATNDT010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696515"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VN788_08860"
     CDS             <1..362
                     /locus_tag="VN788_08860"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HXU20676.1"
                     /translation="IPILHWLMPHAAMVALLKVHLVIRKCGHFTEYFILSLLVLRGIR
                     AGKRDVRLRWSLAAIAIIACYASLDEFHQIFVPGRTPAVGDVLLDSIGGICAQIAFAL
                     VMSKKAAPVNRERAEAP"
     gene            complement(401..1783)
                     /locus_tag="VN788_08865"
     CDS             complement(401..1783)
                     /locus_tag="VN788_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU20677.1"
                     /translation="MKTDNKPSILVVEDETKLRRLLELQLAEEGFQTRIVPDAEAGLQ
                     LLNKEPFDLILTDFKLPSMNGLDFLLAVKRINANLPVVLMTAYGTVESAVGAMKAGAS
                     DYVLKPFSLAELMLVIRKELASHQLRQENRDLREALGRRYEYKNIIARSDKMQAVLGL
                     VERAAPTNATVLVGGESGVGKDLVARAIHEHSNRASGPFVKINSTAIPENLLESELFG
                     YEKGAFSGATSAKPGKFEMADKGTLFLDEIGDVPPPTQVKLLRVLQDREFERLGATKT
                     LKVDVRLIAATNRDLRDALEEGTFREDLYYRLNVVAIDIPPLRERKEDIPPLAQFFLD
                     KFARESGKPVRGITPQAMQALEDFHWPGNVRELQNIIERGVTLSSGGMLDVADIRLDR
                     SVGRATAEAALVLPQGFTLEQWEDEMIREALRHANGNKSQAARSLGLSRNALRYRLSK
                     IGVPDPPEKD"
     gene            complement(1838..>2732)
                     /locus_tag="VN788_08870"
     CDS             complement(1838..>2732)
                     /locus_tag="VN788_08870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU20678.1"
                     /translation="PAHWEYEITVQSLYDLAIRVLFFFLAAMLVNRFVVENRRQVARY
                     QALSQTLEETNRQLERAEAEARRSERLAALGQMSAGLAHEIRNPLGVIKGSAEMLTQK
                     LQDASPLASELAGYISSEVNRVNALVARFLDFARPLQLELHPHQISKIIDSALESVQA
                     QFPATIRIERCYAPGLPDVLCDQQLCEHVFVNLIQNAFQAMNGSHTGREMLLRISLKP
                     ETFRGRHGVSATIEDSGPGVPAESREHIFNPFFTSKKEGVGLGLSIVDKIVDDHHGWI
                     RLDTEYQGGARFRLFLPVSPS"
BASE COUNT          575 a          761 c          762 g          634 t
ORIGIN      
        1 ttattcccat tctccactgg ctgatgccgc atgcggcaat ggtcgccctt cttaaagtac
       61 atctcgtgat ccgcaaatgc gggcacttta ccgagtactt tatcctgagc cttttggttt
      121 tgcgcggcat acgtgcaggc aagcgcgacg tccgcctccg gtggtcctta gccgcgattg
      181 ccatcatagc ttgctacgcg tcactcgatg agtttcatca gatcttcgtg cccgggcgta
      241 ccccggcagt cggcgacgtg ttgctggatt caatcggcgg catctgtgcc cagatagctt
      301 tcgcgctggt tatgtccaaa aaagccgcgc ctgtcaatcg ggaacgagcc gaagccccct
      361 aatcggaatt caagtggttc tgctttagcc gccggccgag ctaatccttc tcaggtgggt
      421 ctggcacgcc aatcttcgat aatcgataac gcagggcgtt gcgagaaagg cccagggaac
      481 gagcggcttg gcttttattg ccattggcgt gacgcaacgc ttcgcgaatc atctcatctt
      541 cccattgttc gagcgtaaat ccttgcggca atacgagcgc ggcttcggcg gttgcgcgcc
      601 cgacggaacg atcgaggcga atatcggcga cgtcgagcat cccgccggag gaaagagtga
      661 caccgcgctc aattatattt tggagctcgc gcacattgcc cggccaatga aaatcctcca
      721 gagcttgcat ggcttggggc gtgatacccc gaaccggctt gcccgattca cgggcgaatt
      781 tatccagaaa aaattgagcg agaggcggaa tatcctcttt gcgctcgcgt aggggcggaa
      841 tatcaatggc tacgacgttt agacgatagt agagatcttc tcgaaatgtg ccttcctcaa
      901 gggcatcgcg gagatcgcga ttggtggcgg cgatgaggcg aacatctact tttagtgtct
      961 ttgtggcgcc gaggcgttcg aactcgcggt cctgaagtac gcggagaagc ttcacctgcg
     1021 tcggaggagg aacgtcgccg atttcatcga ggaatagcgt tcctttgtcg gccatttcga
     1081 acttcccagg ttttgcggat gttgccccgg agaaggcgcc tttttcgtaa ccaaaaagct
     1141 cactttcgag aagattctca ggaatggcgg tgctgttgat ttttacgaag ggtccggaag
     1201 ctcggttcga gtgctcgtga atcgcgcgcg ccaccagatc cttacccacg ccgctttcac
     1261 cgcccacgag gacagttgca ttcgtggggg ccgctcgttc gaccaggccg agaacggcct
     1321 gcattttgtc actacgcgca atgatatttt tgtactcata gcgacgaccc agcgcttcgc
     1381 ggagatcgcg attctcctgc cgcagttgat gcgacgcaag ttccttcctg atgacgagca
     1441 tcagctcggc aagcgaaaac ggtttcaaaa cgtaatcgct cgcaccggct ttcatggcgc
     1501 cgaccgccga ctcgacggtg ccgtatgcgg tcatgagtac cacgggcaag ttggcattga
     1561 tccgcttcac ggccagcagg aagtcgagtc cgttcatgct cggcagcttg aaatctgtga
     1621 gaatgaggtc aaacggttct ttgttgagta actgcaggcc ggcttccgca tcgggcacga
     1681 tacgcgtctg gaagccttcc tccgctaatt gtaattcgag caaccggcgc agtttggtct
     1741 cgtcttctac caccaagatt gagggtttgt tgtcagtttt catgtacgcg ttccggccgc
     1801 acccgacttg ataactcggc cagctaagtc aattgtttca cgaaggcgag actgggagaa
     1861 ataagcgaaa gcgcgcgccg ccctgatatt cggtatcaag ccgaatccag ccatgatgat
     1921 cgtcaacaat tttgtcgacg attgacaggc ccagccctac tccttccttc ttcgacgtga
     1981 aaaacggatt aaagatatgc tctcgtgatt ccgccgggac cccgggacct gaatcctcaa
     2041 tagtggcgct cacgccgtgg cggccgcgaa atgtttccgg tttcagggat attctcagca
     2101 gcatttcgcg tccggtgtgg gagccgttca tcgcctgaaa tgcattctga atcaggttca
     2161 caaagacgtg ctcgcagagt tgctgatcgc acaggacatc cggcagcccc ggcgcatagc
     2221 accgctctat tcgaatggta gccggaaatt gagcctgtac ggattcgaga gcgctgtcga
     2281 tgatttttga aatctggtgc ggatgcagtt caagctgcaa ggggcgcgcg aaatccagaa
     2341 atcttgccac cagagcgttc acacggttca cctcggagga gatgtatccc gcgagttcgc
     2401 ttgccagagg cgaggcatcc tgcagctttt gggtgagcat ttccgccgat cctttgatca
     2461 cgcccagcgg attgcggatt tcgtgcgcga gtcccgccga catctgcccc agcgcggcga
     2521 gacgctcaga ccggcgggct tccgcttctg cgcgttccaa ctgacgattc gtttcctcga
     2581 gggtctggga aagcgcctga tatcgcgcta cctgacggcg attttccacc acgaagcggt
     2641 ttaccagcat cgcagcgagg aagaaaaaca agactcgaat cgccaaatcg tacagacttt
     2701 gaacagtaat ctcatattcc caatgtgcgg ga
//
LOCUS       DATNDT010000273         4229 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3288230, whole genome shotgun sequence.
ACCESSION   DATNDT010000273 DATNDT010000000
VERSION     DATNDT010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288230"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..738
                     /gene="accD"
                     /locus_tag="VN788_08875"
     CDS             <1..738
                     /gene="accD"
                     /locus_tag="VN788_08875"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HXU20679.1"
                     /translation="NVCPKCEKHFRIDARARLSLLLDADQIELFGDNLVSTDPLKFVD
                     LKAYSSRLKQAQEDTGLSDAVINARGTLNGRHVIVSAMEYAFIGGSMGAVVGEAITRA
                     IEQAHDSKTPIIIVSASGGARMMEGVISLMQLAKISAALSRLDDARVPYISVLTDPTT
                     GGVTASFAMLGDLNIAEPGALIGFAGPRVIEQTIRQKLPQGFQRSEFLLEHGMLDAVV
                     PRKQLKAYISRALDFMDKAGASSHATA"
     gene            complement(831..3578)
                     /gene="metH"
                     /locus_tag="VN788_08880"
     CDS             complement(831..3578)
                     /gene="metH"
                     /locus_tag="VN788_08880"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HXU20680.1"
                     /translation="MTTSGDQQNSGGSPTAPIETKPLRLSGSQPFTQQAGVYIMIGER
                     TNVAGSPKFAKLIKAGKYEEAVSIARQQVENGANVLDICMDEGMIDGVTAMTRFLQLL
                     GSEPEVAKVPFMIDSSKWAVIEAGLKCLQGKGIVNSISLKEGEDVFRRNAATVLKYGA
                     AVVVMAFDEQGQAATYEDKIRICERAYRILVDEVGFPPEDIIFDPNILTVATGMEEHN
                     NYAVDYIKASRWIKANLPHAKVSGGVSNVSFSFRGNNVVREAIHSAFLYHAIVAGMDM
                     GIVNAGMLEVYEEIEPELKVMVEDVLLNRRPDATERLVEHGETLKNAGAEVSEKKAEE
                     WRHGTVEERLSHALVKGIDTYIELDAEEARAKLGRPLLVIEGPLMAGMGVVGDLFGAG
                     KMFLPQVVKSARVMKKAVAYLTPFMEAEKAALAASGQEVKAQGKIVLATVKGDVHDIG
                     KNIVGVVLACNNYEVIDLGVMVACEKILERAKAEKADLIGLSGLITPSLDEMVHVARE
                     MERQGFKLPLLIGGATTSRAHTAIKIAPNYSEPVVHILDASRAVPVTTSLLSDEAKPA
                     FVVKHRAEYEALRTSHAAPRQTTVTLEAARARRTPIVWRPEDIPVPDLIGVRAFDNFP
                     LATLREFIDWSPFFHTWGLKGVYPRIFDHPEHGAQARQIFTEGNALLDRIIKENLITA
                     RGVYGFFPANAVGDDVELYTDNTRTKVLERFHFLRQQANREGSEPCRSLADFIAPKET
                     ALPDYIGAFAVTSGIGLKELCDRFRAENDDYNAIMSEALADRLAEAFAECLHKRARED
                     WGYGREENLSNDDLIHEKYRGIRPAAGYPACPDHTEKGPLWKLLDVQKNTGMLITESF
                     AMWPGSSVSGLYFAHPDSRYFVLGKIDRDQVADYHERKGMSVAEVERWLGQNLNYDPA
                     Q"
     gene            complement(3575..>4229)
                     /locus_tag="VN788_08885"
     CDS             complement(3575..>4229)
                     /locus_tag="VN788_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HXU20681.1"
                     /translation="RQRFVAGAIGPLTVSLSNSPDADDAGFRVVTFDQVKTAYAQQVR
                     GLIAGGVDALLVETIFDSLNAKAALVAIREVFDEDGKNLPIMISAAVGRGGETMISAQ
                     TTEAFWNAVAHVKPLSVGLNCSLGPDLMYPFLSELSEKADVAISCYPNAGLPNPLSPT
                     GFDLQPADMARYLGDFARAGLINIAGGCCGNTPEHIAAIAKALEGMPPRELRVALKQ"
BASE COUNT          854 a         1241 c         1194 g          940 t
ORIGIN      
        1 aacgtctgcc ccaaatgcga aaagcacttc cgcattgacg cccgcgcccg gctcagcctg
       61 cttttggacg ccgatcaaat cgagcttttt ggcgacaatc tggtttccac tgacccgctg
      121 aagtttgtgg acctgaaggc ctattcgtcg cggttgaagc aagcgcaaga agacaccggt
      181 ttgagcgatg cggtcatcaa tgcacggggc acgctcaacg ggcggcatgt aatagtcagc
      241 gccatggaat acgcctttat cggcggcagc atgggcgccg tggtaggcga agcaattacg
      301 cgggccatcg agcaggcaca cgattcgaag acgcccatca ttatcgtttc cgcctctggc
      361 ggcgcgcgca tgatggaagg cgtgatcagc cttatgcaac tggcaaaaat ctccgctgcg
      421 ctttcgcggc tggacgatgc ccgggtgcct tatatttccg tgcttaccga tcccaccacc
      481 ggcggcgtaa cggcctcatt cgctatgcta ggggatttga acatcgccga acctggcgcg
      541 cttattggat ttgccggccc gcgggtgatt gaacagacca tccgccaaaa gcttccgcag
      601 ggcttccagc gcagcgagtt cctgctggag cacggtatgc tcgacgccgt tgtgccgcgc
      661 aagcagctga aggcttacat ctctagggcg ctggatttta tggacaaggc tggcgccagc
      721 agccacgcca cggcttaact ctcttatagg gatcctccac ttcgctcagg aggacaatag
      781 attagtacgc ggacaggagt gtccgcgcca cacagaaaca gcaggattgc ctattgcgcg
      841 ggatcgtagt tcaaattttg tcctagccat cgctcgactt ccgctacgga cattcctttg
      901 cgttcgtgat agtcggcgac ctggtcgcgg tcaattttgc ccagaacaaa ataccgcgag
      961 tcgggatggg cgaagtagag tccgctgacg cttgaaccgg gccacatggc gaacgactcg
     1021 gtgatgagca ttccggtatt tttttgcacg tcgagcaact tccagagcgg gcctttttcc
     1081 gtgtggtcgg ggcacgcggg gtaacctgct gcgggccgga tgccgcgata tttttcgtga
     1141 atcaaatcgt cgttgcttag attttcctcg cgcccgtagc cccagtcttc ccgcgcccgt
     1201 ttgtgcaggc attcggcaaa agcttcggcg agacggtcgg cgagggcctc ggacatgatt
     1261 gcgttgtagt catcgttttc cgcacggaag cggtcgcaca gctctttcag cccgattccg
     1321 cttgtgacgg cgaatgcgcc gatgtaatcc ggcaaagccg tttctttcgg tgcgatgaaa
     1381 tcggcaagtg accggcaagg ctcgctgcct tcacggtttg cctgctggcg caggaagtgg
     1441 aagcgttcga gaactttggt acgagtgtta tccgtgtaca gttcgacgtc atcgccgact
     1501 gcatttgcag gaaagaatcc gtaaacgccg cgcgcggtga tcaggttctc cttgatgatg
     1561 cgatcgagca gtgcgttgcc ttcggtgaat atctgccgcg cctgtgctcc gtgttcggga
     1621 tgatcgaaga tccgcggata aacccccttt agtccccagg tatgaaagaa gggcgaccag
     1681 tcgatgaatt cgcgcagcgt tgccaaaggg aaattatcga acgcacggac gccgatgaga
     1741 tcaggcacgg ggatgtcttc gggtcgccac acgattggag tgcgccgggc gcgcgcggcc
     1801 tcaagagtaa cggtcgtttg gcgcggtgcg gcgtgggacg tgcggagcgc ctcatattcc
     1861 gcgcgatgtt tcaccacgaa cgcgggcttg gcttcgtcgc taagaagact tgtagttacg
     1921 ggcacggcgc ggctcgcgtc cagaatgtgc accaccggct cactatagtt aggcgcaatc
     1981 ttgatggcgg tatgggcgcg gctcgtagtc gcgccgccaa ttaggagcgg caacttgaac
     2041 ccctggcgtt ccatttcgcg ggcaacgtgc accatctcgt cgagcgatgg agtgataagg
     2101 ccgctgagcc caatcaaatc tgctttttcg gccttggcgc gctccagaat tttctcgcag
     2161 gcaaccatca ccccaagatc tataacttcg tagttattgc aggcgagcac cactccgacg
     2221 atgttcttgc caatatcgtg aacatcgcct ttgacggtgg cgaggacgat ttttccctgc
     2281 gcttttactt cctgacctga cgcagccaga gccgccttct ccgcttccat gaacggcgtg
     2341 aggtaggcca cagccttctt catgacccgc gccgacttca ccacctgcgg caaaaacatt
     2401 ttgcctgcgc cgaacagatc accgacgaca cccatacctg ccattaacgg cccttcaatc
     2461 acgagtagcg gacggccaag tttggcacgt gcttcctcgg catcaagctc gatgtacgta
     2521 tctattccct ttaccagcgc gtgagagaga cgctcttcca ctgttccgtg gcgccattct
     2581 tcagccttct tttcgctgac ttccgccccg gcgttcttta gtgtttcacc gtgctcaacc
     2641 aaacgttcgg tcgcatcagg gcggcggttg agcagaacat cttcgaccat caccttcagt
     2701 tcaggctcaa tctcttcgta cacctcgagc atgccagcgt tcacaatgcc catgtccatg
     2761 ccggcgacaa tggcgtgata gagaaacgcg gagtggatgg cctcgcggac gacattgttt
     2821 ccgcgaaaac tgaaagaaac gttcgagacg ccgccgctga ctttcgcatg cggcaggttc
     2881 gccttgatcc agcgcgaggc cttgatgtag tccactgcgt agttgttgtg ctcctccatg
     2941 cccgtggcca cggtcaggat gttgggatca aagataatgt cttctggcgg aaagccgact
     3001 tcatcaacca agattcgata ggcgcgctca cagatgcgga ttttatcttc gtaggtcgcg
     3061 gcctggccct gctcgtcgaa cgccatcact acgaccgccg cgccatactt aagcacggta
     3121 gctgcatttc gccggaagac atcttcgcct tccttcagcg aaatcgaatt cacgattccc
     3181 ttgccttgta ggcatttgag tcccgcctcg atgactgccc actttgagga gtcaatcatg
     3241 aacgggactt tggcgacttc cggttcgctg cccagcaatt gcaggaagcg cgtcatggcc
     3301 gtgacgccgt caatcattcc ttcgtccatg cagatgtcga gaacattcgc gccattctcg
     3361 acctgctggc gagcgatgct gaccgcctcc tcgtattttc cagccttaat cagctttgcg
     3421 aacttgggag agccggccac attggtccgc tccccgatca tgatgtaaac acccgcctgt
     3481 tgcgtgaagg gctgtgaacc cgataggcgc aggggtttgg tttcgatggg cgcggttgga
     3541 gagccgcctg agttctgttg gtcgccggat gtcgtcattg tttcagcgcc acccgcagtt
     3601 cccgtggagg catgccttcg agggccttcg cgatggccgc gatgtgttcg ggcgtgttgc
     3661 cgcagcatcc tcccgcgatg ttgataagcc ccgcgcgcgc aaaatccccg agataacgcg
     3721 ccatgtccgc gggctggaga tcgaagccgg tgggagacag aggattcggc agcccggcat
     3781 ttggatagca cgaaatcgcc acgtcggctt tttccgacag ctcactcaaa aagggataca
     3841 taagatcagg accgagagaa caattgagcc cgactgacag cggcttcacg tgcgccaccg
     3901 cattccagaa cgcctcggta gtctgcgcag agatcatcgt ctcgccgcca cgcccgacgg
     3961 ctgctgaaat cataatcggc aaattcttac cgtcctcatc gaacacttca cggatggcaa
     4021 caagcgctgc tttggcgtta agggaatcga aaattgtctc gacaagtagt gcatccaccc
     4081 cgccggcaat cagaccacgc acctgttgcg cataagcggt tttcacctga tcgaaggtga
     4141 ccacgcggaa gccagcatcg tcagcatccg gcgagttcga aagggacacc gtcaagggtc
     4201 cgattgctcc cgctacgaat cgctgacgg
//
LOCUS       DATNDT010000274         6845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2641682, whole genome shotgun sequence.
ACCESSION   DATNDT010000274 DATNDT010000000
VERSION     DATNDT010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6845
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2641682"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VN788_08890"
     CDS             complement(<1..576)
                     /locus_tag="VN788_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit B"
                     /protein_id="HXU20682.1"
                     /translation="MSLTREGVAYLVACDLPEGSYVNLGIGIPTLVSGFVPADREVFF
                     HSENGILGMGPLAEPGHEDPDIINAGKQYVTLIPGACVFDHPTSFSMIRGGHINYAVL
                     GALEVAANGDMANWRTPGEKVPGVGGAMDLAFGAQNVFVTMTHVTNKGKPKIINKCSL
                     PLTAPRCVKRIYTDMAVIEVTPEGLVLREFAP"
     gene            complement(580..1281)
                     /locus_tag="VN788_08895"
     CDS             complement(580..1281)
                     /locus_tag="VN788_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacid CoA-transferase subunit A"
                     /protein_id="HXU20683.1"
                     /translation="MVNKTLATCREAVAGVFDGATILLGGFGDPGLPGQMLEALREQG
                     AKNLTMVHNGSGGGTHALGGLIHDGRVRKLIASFPNNPGGTSFQELYLKGQIELELVP
                     QGTLAERIRAAGAGLGGFFTPTAAGTELAKGKETRMIDGREYVFEKPLHGDFAFVRAH
                     RADRLGNLNYRMAMRNFNVPMATAAKFVAAEVDELVPVGDLDPEDIHTPGIFVDRVVE
                     VVRHPKYLEITTEGR"
     gene            complement(1442..2251)
                     /locus_tag="VN788_08900"
     CDS             complement(1442..2251)
                     /locus_tag="VN788_08900"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXU20684.1"
                     /translation="MGATQQIASALVLEERSGAVITVRLSRPEKLNALNPEMCRALVH
                     ALLRAADDRSVRVVVLTGAGRGFCSGGDINYIHEARARRSAREFEALLSAGKEICLAI
                     ASMPKLVIAAVNGPAAGGGMSLALACDLRIASDQAMFKQAFAQLGLYPDLGATFFLPR
                     LVGLARASELFYTSERLSAEEARQIGMVYRVYPHEQFSAETQRFAQELAEGPPLAYRD
                     VKRTMIGEAQQALSEAIDEENRLQLHCFLSEDCGEGLNAFLEKRAPRFHGH"
     gene            complement(2282..2794)
                     /locus_tag="VN788_08905"
     CDS             complement(2282..2794)
                     /locus_tag="VN788_08905"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HXU20685.1"
                     /translation="MRTLPDYLRKGMKMILVGANPGDRSARVGHYYAGRNNQFWPMMY
                     QSGVIPEPIGYEDDRRMLEFGIGMTDLVKRPTRGTDEIERQEFAEGRVLLAQKLEDMR
                     PRVVAFNGKMVYEKFAGRPCKFGLQKELLYGARVFVLPSTSGQNGGTSAGVKKRYFKQ
                     LAALLKALKD"
     gene            2990..3646
                     /locus_tag="VN788_08910"
     CDS             2990..3646
                     /locus_tag="VN788_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU20686.1"
                     /translation="MNSPKLDPVEGHVLPSQVVKADEPILVAAAKNGDISAFETLVGR
                     YERKIFRLTQNITQNREDAEDAMQEAFLKAFQHLGEFQGNSRFYTWLVRIAVNQALMK
                     LRKRRPNVVSLDEEIDTGEDMMPRDVEDWGPSPQQRYEQTELSEILGKVIGELESPFR
                     VVFQLRDIEEMSTEETAEALGLSVPAVKSRLLRARLKLRQKLNQYFKQGERREVQKNH
                     "
     gene            3624..3863
                     /locus_tag="VN788_08915"
     CDS             3624..3863
                     /locus_tag="VN788_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXU20687.1"
                     /translation="MKCKKIINELADYLDESLDPILRSSIEEHLGNCKDCRIVVDTTK
                     QTIEIFCNSEPAPLPLTTRQRLHEALAKHLRHLRA"
     gene            complement(3926..4903)
                     /locus_tag="VN788_08920"
     CDS             complement(3926..4903)
                     /locus_tag="VN788_08920"
                     /inference="COORDINATES: protein motif:HMM:NF014476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HXU20688.1"
                     /translation="MALFLTEEDVKSILTMPLALEAVETSFRRLADGSAISHSRQRLR
                     MVGKGFLHYMAAADSAGGYMGLKIYSVSPSGARFLVPLMSAQSGEMLALIEANYLGQV
                     RTGAASGLATRLMAREDARTAGIVGTGLQARTQLEAVALARKLEKVHAFSRDAARREQ
                     FASEMTARLGVPVAAVGTAEEAVRGADILITSTTSTKPVVEGKWLAPGVHINAIGVNF
                     AEKRELDAEVVARCDVIAADSVEQSKLEAGDLIQAFGNDASRWAAVRELGQIVSGKIP
                     GRTNRTQITLFKSNGIAIEDVVVAGRIYEMARQRKIGREIPLFEKWQEK"
     gene            complement(4954..5991)
                     /locus_tag="VN788_08925"
     CDS             complement(4954..5991)
                     /locus_tag="VN788_08925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NHL repeat-containing protein"
                     /protein_id="HXU20689.1"
                     /translation="MALRQDENMPAGKIIFEGGGLYVGSKSRVGWSSLRGHRPIAILL
                     VATAVVSLCWFVGCSRAATRADSQKSVRPPISYIGSWGMHGDGPGQLDLPASIATDRV
                     GDVYIPDAGSNFVHKFDRRGTPLLSFQYPGLTDPQSVALDSGGAIYVTDSHRGSAFIF
                     LPSGDRYRELHLNTRPKPDDELGVAVGDDGVVHVLDPDASRIFTYNSRFRLARAWSPV
                     AGSPSDKVRASSIAVGPDGYLYMADRAENRILRFTEDGHFAGEFKCGANRRLSDQFAL
                     MRGFIFAMDADGRKLHVWSTDGQPKLDVDLAPELGQANRPAPPIAVSVHKELLVLDAP
                     EARVLRYQINF"
     gene            complement(5954..>6845)
                     /locus_tag="VN788_08930"
     CDS             complement(5954..>6845)
                     /locus_tag="VN788_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXU20690.1"
                     /translation="TLWSTSAQVREPTGIVNYGERLEILDRFQDQLHVRTTNGLTGWI
                     TQGDLLTSEMWQKAKDLESETKTLPIEARGHTRALTNLHIAAGRESPRIRQLNKMVPV
                     DLFKRQVVAFPLAPTAVSIKEADARVPTAKKEDWWLVHAHLSDGTMVSGWLLGKFVEL
                     DVPSPLPDYANSAGMHIVAWFELNRVMDEASGERKPQYLLVGSRGAEGQPCDFTLMRV
                     YTWGKKRERYETAFVESNLCGKLPVKLTEPPAPSADVTFSFQDWSAGAAQQRTYVMRQ
                     TVVKQLRQDGAAPRRKHARR"
BASE COUNT         1505 a         2056 c         1844 g         1440 t
ORIGIN      
        1 gggcgcaaat tcgcgaagaa cgaggccctc gggcgtaacc tcgataacgg ccatatccgt
       61 gtaaatgcgc ttcacacaac gcggagcggt caacggcaac gaacatttgt tgatgatttt
      121 cggtttcccc ttattggtca catgggtcat ggtaacgaaa acattctgcg cgccgaaggc
      181 aagatccatc gcaccgccca caccaggcac cttttcgcca ggagtgcgcc agttggccat
      241 atccccattc gccgcaactt ccagcgcgcc cagcacggca taattgatgt ggccgccgcg
      301 aatcatggaa aacgacgtgg gatgatcgaa gacgcaggcg cccggaatca acgttacata
      361 ctgtttgccc gcgttgatga tatccgggtc ttcgtgtccc ggctccgcca aaggacccat
      421 gcccaggatt ccgttttcgc tatggaagaa gacctcccgg tccgctggca cgaatccgga
      481 gacgagagtg ggaatgccaa tgccaagatt tacgtagcta ccttcaggaa ggtcacaggc
      541 gacgaggtaa gcgacgcctt cgcgagtgag tgacattgtt tatctcccct ccgtggtgat
      601 ttcgagatat ttcggatggc ggacgacttc aacgacgcga tcaacgaaaa tgcccggcgt
      661 gtgaatatct tcgggatcga gatctcccac gggaaccagt tcgtcgactt ccgcggcgac
      721 aaattttgcg gcggtcgcca tcggaacgtt gaaattgcgc atggccatcc ggtaattcaa
      781 attgcccagc cgatcggctc ggtgggccct cacaaaagcg aaatcgccat ggagaggctt
      841 ttcgaaaacg tattcgcgtc cgtcgatcat gcgagtttct ttgcccttgg ccaactcggt
      901 gccggcagcg gtgggtgtga aaaaaccacc aagcccggcg cctgcggcac gaatccgctc
      961 ggcgagagtg ccctggggaa ccaattccag ctcgatctga ccttttaagt atagctcctg
     1021 gaatgaggta ccgccgggat tattcggaaa ggaggcaatc aacttccgca cccggccatc
     1081 gtgaatcagc ccgccgagag cgtgcgtacc tccgccggaa ccattgtgga ccatggttag
     1141 attcttggcg ccttgttcgc ggagggcttc gagcatctgg ccgggcaagc cgggatcgcc
     1201 aaagccgccc agcaaaattg tggctccgtc gaaaacacct gcaactgctt ccctgcaggt
     1261 agctaaggtc ttgttcacca tatccctatc ctaattttct cgtcgcaatt tagcacgcgc
     1321 tgcgttcagg aatcaaatgg tgccaggtat aaaacgaaaa ccggagttgg tctgggagca
     1381 ctcagacaag atcactgcct agctcaagcg gaatataact tagcgagttt cacgcctgcg
     1441 atcagtggcc gtgaaaacga ggcgcgcgtt tctcaagaaa agcgttaagc ccctcgccgc
     1501 aatcttcgga gagaaaacag tgaagctgca gacggttttc ttcgtcgatc gcctcgctaa
     1561 gagcttgttg cgcttcacca atcatggtgc gctttacgtc cctgtaggca agaggcggcc
     1621 cttcggcaag ctcttgagca aatctctgcg tttccgcact aaattgctca tgaggataga
     1681 cgcgatagac catgccaatt tgccgcgcct cctcggcgga gaggcgctcc gaggtgtaaa
     1741 aaagttcgga cgcacgcgcc aatcctacga ggcgcggcag gaaaaaagtt gcgcccaagt
     1801 cgggataaag ccccagttgc gcgaacgcct gcttgaacat ggcttgatcc gaggcaatgc
     1861 gcaagtcgca agcaagggcg agactcatgc cgccgccggc agccggaccg ttgacggctg
     1921 cgatcacgag tttgggcatc gaggcaatcg cgaggcagat ctctttcccg gcgcttagca
     1981 acgcttcgaa ctcgcgcgcc gagcgccggg ctcgcgcctc gtgaatgtag ttgatgtcgc
     2041 ccccggaaca gaaaccgcgt ccggcacccg tgagcactac aactctaacg gatcgatcgt
     2101 ccgccgcgcg aagaagcgcg tgcaccagcg cgcggcacat ttctggattg agcgcgttta
     2161 gtttttccgg ccgacttagg cgaacggtaa tcacagcgcc agatcgctct tcgagcacca
     2221 gcgcactcgc gatttgttgc gttgcaccca cggatgatta atttcgacga agctgaatgt
     2281 tctagtcctt cagcgccttc aacaaggcag ccagctgttt gaagtatctc ttcttcacgc
     2341 cggcgctggt gccgccattc tgtccgcttg tcgagggcag cacaaaaacg cgggcgccgt
     2401 aaagcagttc cttctgcagt ccgaacttgc aaggacggcc cgcgaacttt tcatagacca
     2461 tcttgccatt gaaggcgacc acgcgcggac gcatatcctc cagtttttgt gctagcaaca
     2521 ctctgccctc ggcaaactcc tgccgctcga tttcgtcggt gccccgggtg ggccgtttta
     2581 cgaggtccgt catgccaata ccgaattcga gcatccgccg gtcatcctcg tatccaatgg
     2641 gttcggggat cacaccagat tgatacatca tcggccagaa ctgattgttg cggccggcat
     2701 agtagtgtcc cacgcgcgcg gagcgatctc ctggattggc gcccaccagg atcattttca
     2761 ttcccttgcg caggtaatcc ggcaatgtcc tcatagaaag tctaaattcc ttccatcgat
     2821 ttcgcgcccg ttgcgtctga taagtgcaat cggcggaatc cagagcaaac cttattgccg
     2881 agcttaaagt gcgccacgtg ctgcagtttg cgaccggcgg ttatcataat acattcccaa
     2941 cagcaggggt tcgaaagctg tgctaccctt ttctacgaaa gaaaccctga tgaattcgcc
     3001 aaaattggac ccggttgagg gtcacgtgct gccatcgcag gtggtgaagg ctgacgagcc
     3061 gatattagtc gccgcggcga aaaatgggga catttccgcc ttcgagacgt tggtcggccg
     3121 atacgagcgc aaaattttcc gtctgacgca aaatatcacg caaaaccgcg aagatgccga
     3181 ggacgccatg caggaagcgt tcctgaaggc gttccagcac ctcggtgaat ttcagggcaa
     3241 ttcgagattc tatacgtggc tggtgcgaat tgccgtgaat caggcgctga tgaaactgcg
     3301 aaagcgtcgg ccgaatgtgg tctcgctcga cgaagaaata gatactggcg aggatatgat
     3361 gccgcgtgac gtcgaggact ggggaccttc tccgcagcag cgctacgagc aaaccgagct
     3421 ttcggaaatt ctcgggaagg tgatcggaga actcgaatcg cccttccgcg tagtcttcca
     3481 gttgcgcgat atcgaagaaa tgtccaccga ggaaacggct gaggcgttgg ggctttccgt
     3541 tccggcagtc aagtcgcggc ttttgcgagc ccggctgaaa ttgcgccaga aattgaatca
     3601 atactttaag cagggggaac gccgtgaagt gcaaaaaaat cattaatgaa cttgcggatt
     3661 acctcgacga atcgctggat ccaatcttgc gctcgtcgat tgaggagcac ctcggaaact
     3721 gtaaggactg ccgtatcgtt gtggacacca cgaagcagac catcgagatt ttctgcaact
     3781 ccgaacccgc gccgttaccg ctaacgactc gccagcgact ccacgaagca ctcgccaagc
     3841 accttcgcca cttgcgcgcc tgatcagcga aaagttctca ggccgcgcct tcctcgcctc
     3901 tgccttcccg cccccgctgc tttgattatt tctcctgcca tttttcgaac aacggtattt
     3961 cgcgcccgat tttccgctgg cgcgccattt catagattcg accagcaacc actacgtctt
     4021 caatagctat tccatttgat ttgaagagcg tgatttgggt gcggttcgtg cggcctggaa
     4081 tcttgcccga aacaatttgc ccaagctcac ggacagccgc ccaccgcgag gcgtcatttc
     4141 caaacgcttg aatcaaatcg cccgcttcga gctttgactg ctccacggaa tcggccgcga
     4201 ttacgtcaca ccgagcgaca acctccgcat ccagctcccg cttttcggcg aaattcacgc
     4261 ctatagcatt gatgtggacg cccggcgcaa gccacttccc ttcgaccacg ggctttgtgg
     4321 aggtagttga cgtaatcaga atatcggcgc cgcgcaccgc ttcttccgcc gtcccaactg
     4381 cggccacagg aacgcctaac cgcgccgtca tttcgctcgc aaattgctcg cgccgcgcgg
     4441 cgtcacggct gaacgcgtgc accttttcca gtttcctagc gagcgcgaca gcttcgagct
     4501 gcgttcgcgc ctgcaagccg gtcccgacga ttccggcggt gcgcgcatct tctcgcgcca
     4561 tcaatcgagt ggctaagccg ctcgccgcac ctgtgcgcac ctggcctaaa tagtttgcct
     4621 caatgagcgc gagcatttca cccgattggg cgctcataag cgggaccaga aagcgcgcac
     4681 cgctcggcga cacagaataa attttcaggc ccatgtatcc gcctgcgcta tcggcggccg
     4741 ccatgtagtg caaaaagcct tttcctacca tgcgcagcct ctgccgcgag tgcgagatgg
     4801 cgctgccgtc tgcgagccgg cgaaacgaag tctcgacggc ttcgagcgcc aacggcattg
     4861 taagaatcga tttcacatcc tcttcggtca aaaacagtgc catcgcgtga accctcctca
     4921 aactcgatcg aattaaaatg gctgaaggaa acttcagaaa ttgatttgat agcgaaggac
     4981 ccgcgcttcc ggagcatcca gaaccaggag ttccttgtgc acgctcacag cgatcggagg
     5041 cgcgggccga tttgcctggc caagctcggg ggcaagatcc acatccagtt tgggctgccc
     5101 gtctgtggac catacatgca gcttgcgtcc gtcggcatcc atcgcgaaaa tgaatccacg
     5161 catcagcgcg aattggtcgc tcagccggcg attcgcgccg cacttgaact cgcccgcgaa
     5221 atgcccgtct tcggtaaagc gcaggatgcg attctccgcg cgatcagcca tgtacaagta
     5281 gccgtcagga ccgaccgcaa tcgagctcgc tctaactttg tcactcgggg agcccgccac
     5341 cggggaccaa gctcgcgcaa gcctaaagcg ggagttatag gtaaaaatgc gcgacgcgtc
     5401 cggatccaaa acgtggacta ctccatcatc gccaacggcc acccctagtt cgtcgtcggg
     5461 cttcggacgc gtattcaaat gcaactcgcg atagcggtct ccgctcggaa gaaaaatgaa
     5521 cgcgctgccg cgatgtgaat cggttacgta aatcgctccg ccgctatcaa gggcaaccga
     5581 ctggggatca gtaagtcccg gatattgaaa cgaaagcagc ggagtgccac gccggtcaaa
     5641 cttgtgaaca aaattgctgc ccgcatccgg tatgtaaacg tcgcccactc gatctgtggc
     5701 aatcgacgcc ggtagatcga gctgtcccgg gccgtcgccg tgcatgcccc agctgccgat
     5761 gtaggaaatc ggcggcctaa cggacttctg cgaatcggcc cgcgtcgcgg ctcgcgaaca
     5821 tccaacaaac caacacagtg acacaaccgc cgtggccacc aggagaattg ctattgggcg
     5881 atgcccgcgc aatgagctcc agccaactct tgacttagaa ccgacataca acccgccgcc
     5941 ttcgaagatg attttacctg cgggcatgtt ttcgtcttgg cgcagcgcca tcttgtcgaa
     6001 gttgcttcac aactgtctga cgcatcacat aagtgcgctg ctgcgccgcg cccgcgctcc
     6061 aatcttgaaa tgagaacgtc acgtcggcag aaggcgctgg aggctccgtc agtttcacgg
     6121 gtagctttcc gcacagattg ctttcaacga aggcggtttc gtaccgctcc ctcttttttc
     6181 cccaggtgta cacgcgcatc aaagtgaaat cgcaaggttg gccctcagcc ccccggctgc
     6241 ccacaagaag atactgaggc tttcgctcgc cagaggcctc gtccatcacg cggttcaatt
     6301 cgaaccacgc cacgatgtgc attccggccg agttcgcgta gtcgggcagc ggcgacggca
     6361 catccaactc aacgaacttt cccagcagcc atccagatac catcgtcccg tcggacaagt
     6421 gcgcgtgaac gagccaccag tcttcttttt tggctgtcgg cacccgggca tcagcttcct
     6481 tgatgcttac agcggtgggg gccagcggaa acgcgaccac ctgcctcttg aacaagtcaa
     6541 cgggaaccat tttattaagc tggcggatcc gaggactttc ccggcccgcc gcaatgtgca
     6601 ggttggtgag cgccctggtg tgcccccgcg cctcaatggg aagagtcttc gtttcggact
     6661 ccagatcctt ggctttttgc cacatctccg aagtcaatag gtcaccctga gtgatccagc
     6721 cggtcaaacc gtttgtggtg cggacgtgca gctgatcttg aaatcgatcc agtatttcca
     6781 ggcgttctcc gtaattcaca atccctgtcg gctcgcgtac ctgagcgctt gtgctccaca
     6841 gcgtc
//
LOCUS       DATNDT010000275         4207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_882555, whole genome shotgun sequence.
ACCESSION   DATNDT010000275 DATNDT010000000
VERSION     DATNDT010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882555"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            12..137
                     /locus_tag="VN788_08935"
     CDS             12..137
                     /locus_tag="VN788_08935"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20691.1"
                     /translation="MFALDEKLDLGPDATRAQVMDAIQGHILGKGVLVGRFHRPQ"
     gene            complement(189..1424)
                     /locus_tag="VN788_08940"
     CDS             complement(189..1424)
                     /locus_tag="VN788_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HXU20692.1"
                     /translation="MRKLLGITLGIMTALGGFVDLGQIVFTTQAGALFGYQLLWSIIL
                     GTVGIVVYMEMCGRVAVVAGEPVFAVVRTRLGTKLGLVTLVASNLLNLITCAAEIGGL
                     AILLHLLTGWPERALLIGSCLAIAAIIYASSFQWIERSFGLSGLLMVVFGISAAVLHP
                     RWSSLIAGLNPAMHAASGKQILLYAYFAVGIFSAMLMEYEVHFYSTGAIEEDWKPNDL
                     GENFMVAMLGSVLGSILTIALMVLGALLFLPHHVFPQMLSTSIMAGAFPFAGKALTIA
                     LIGAMACIGGAAVETALSGAYNVCQFFNFAWGKNRPAKEVPVFTAGWVGMLALALIIG
                     LTGLRPLTLVNIAIIFGMVVMPFTYYPILSAAADKKIMGKHVNSRAITIVGGVFLVLI
                     VAAAIAAIPLMWLTHAGQP"
     gene            complement(1421..1864)
                     /locus_tag="VN788_08945"
     CDS             complement(1421..1864)
                     /locus_tag="VN788_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20693.1"
                     /translation="MHLLRHRTVLCIKAFFLLSAIALVSCAGRKTLSSEELHSDVVSA
                     ISLASETEAFAGQIEQGRLTREFQSGHAAYLRDEAERQAKELRKSAPQPNQAQTVHSC
                     IEQLDLLARDLARIAKTTTDKTQIPAIREDVQRVVQSLTGLKAQL"
     gene            complement(2096..2710)
                     /locus_tag="VN788_08950"
     CDS             complement(2096..2710)
                     /locus_tag="VN788_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20694.1"
                     /translation="MCRLSCRLLLFVSVLVLFFSIVPGVAAQCAPSLKAVNPAVFHMS
                     SGRLQLELVRADRNHGNDNDSSIVGLWHTNYTATFDDNFPPGAPATAPFPFNQTFKIW
                     HADGTEFDNAFLPPAGGNICYGVWKDMGRNSVKVHHVGVMYDPSGNVSAIFYIDEVDT
                     LALDGKTYSGTFDFKLFAPDNIFGVGTPISEVKGTVAATRITVN"
     gene            2837..3802
                     /locus_tag="VN788_08955"
     CDS             2837..3802
                     /locus_tag="VN788_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20695.1"
                     /translation="MDAFLSWRKTRPSDLAHCLALHPAKNGAEIVGRARVLEGWRKLL
                     EMRHATRSAVVEIHTKGRAELVGFGLASFVKEDFVRDEVQKPRPGLNSRLIDSVVNGN
                     PVVATYAEVREANTRGDLHQVILDTSWKNGPLTPVQVDEVRVLLGRAYQELFAGYHFS
                     RILVELVDELDSWHIRGLKVFRTIDEFEDFYRDNPATKWNRNRRLIDVTLDSIRADPH
                     SVAAELFQHHLRPQLALTRGEQELLELALDGVEDAAAAESLFVSVAAVKRRWASLFER
                     VGAIRPDLCPMDGEGTRGIQKRQRILTYVRSHPEELRPFNSSAAG"
BASE COUNT          960 a         1067 c         1287 g          893 t
ORIGIN      
        1 acacgtttga attgtttgct ctggacgaaa agctggacct cggccccgat gccacgcgcg
       61 cgcaagtgat ggatgcgatt caagggcaca ttctggggaa gggcgttttg gtgggacgtt
      121 tccatcgacc acagtgaggc gaccgaggcg atcgttcgat atttaaacag atcagtggct
      181 gtgtgacttt aaggctggcc ggcgtgggtg agccacatca gggggatggc ggcgatggcg
      241 gcggcgacga tcagcacgag aaaaactccg cccacaattg tgatggctcg gctgttgacg
      301 tgctttccca tgattttctt atcggctgcg gcgctaagaa ttgggtaata agtgaacggc
      361 atcacgacca tgccaaaaat gatggcgatg ttgacgagag tgagtgggcg cagaccggtg
      421 aggccgatga tgagcgccag agcaagcatt cccacccatc cagccgtgaa aaccggaact
      481 tctttcgcgg gccggttctt accccaagca aagttaaaaa attggcagac attgtaggct
      541 cctgagagcg cggtttcgac ggccgctccg ccgatgcagg ccatcgctcc gatcagcgcg
      601 attgtgagcg ctttgccggc aaacggaaat gcgcccgcca tgatggatgt gcttagcatc
      661 tgcggaaaaa catggtgcgg caagaagagg agcgcgccca gcaccatcaa tgcgatcgtg
      721 aggatcgagc caagcaccga tccgagcatt gccaccatga aattttcgcc gaggtcgttg
      781 ggtttccagt cttcctcaat ggcacccgtg gaatagaaat ggacttcgta ttccataagc
      841 atggcgctga aaatcccaac ggcaaaatag gcatagagaa gaatctgctt gccactggcc
      901 gcatgcatcg ccggatttaa tccggcaata agcgatgacc agcgcgggtg tagtacggcg
      961 gctgagatgc cgaatacaac catcaggagc cctgaaagcc cgaagctgcg ctcgatccac
     1021 tgaaagctcg atgcataaat gatcgcggcg atggcaaggc aactgccgat cagcaaggca
     1081 cgttcgggcc aacccgtcag aagatgcagc aggattgcga gaccgccgat ctctgctgcg
     1141 caggtgatca ggttgagcag attcgaggcg accagcgtga cgagacccaa tttggtacca
     1201 agacgcgtgc ggacgacggc gaagacgggc tcacctgcga ccaccgccac acgaccacac
     1261 atttccatgt aaacaacgat tccgacggta ccgaggataa tcgaccagag aagctggtat
     1321 ccgaatagag cgccagcctg agtggtgaag acgatctggc cgagatcgac gaagccgcca
     1381 agcgctgtca tgatgcccag cgtgatgccg agcagttttc tcatagttgc gctttcaaac
     1441 cggtgagaga ttgcacgact ctctgcacgt cttcccggat tgccggaatt tgtgttttgt
     1501 cggttgtggt ttttgcgatt cgggcgaggt cgcgtgcaag gagatcgagc tgctcgatac
     1561 agctgtggac ggtctgcgcc tgattgggct ggggagcgga tttgcgtagt tctttggctt
     1621 ggcgctccgc ttcatcccgc agataggcgg catggcctga ctgaaattcg cgcgtgagtc
     1681 ggccttgctc aatctgaccg gcaaacgcct cggtttccga tgcgagcgaa atggccgaaa
     1741 ctacgtcaga gtgcaactct tccgaagaga gcgtcttgcg gcctgcgcac gaaacgagcg
     1801 cgatggcaga gagaagaaag aatgccttta tgcataggac agtgcggtga cggagtaggt
     1861 gcattcgagc gccagggcta aacgtcgcca cagtaggctg actggcaaat taccacttga
     1921 tggttggata tgcgacggat gcggttttgg agatttggcc ggggaaaagc cgcatgaacc
     1981 ggcagcgttg cggtcgctcc gcgaacgcac accgcttgcg atgtgagcgg cagaagggaa
     2041 gccgccgaaa gaggttagac cgccgaccgg agatcggcgg tcccggggaa caggatcaat
     2101 tcacggtgat gcgtgtggcg gctacagtgc ctttcacttc tgatattggc gttccaacac
     2161 cgaaaatgtt atctggagca aagagtttga aatcgaacgt gccactgtag gttttaccgt
     2221 cgagcgcgag tgtgtcgact tcgtcgatgt agaaaatggc gctcacattg ccgctcggat
     2281 cgtacatgac gccgacgtga tggaccttaa cgctgttgcg acccatgtcc ttccatactc
     2341 cgtagcagat gttcccgcca gcgggcggca gaaaagcgtt atcaaactcg gttccgtcag
     2401 catgccagat tttaaaggtc tgattaaatg ggaagggcgc ggtggcgggc gctcccggag
     2461 ggaagttgtc gtcgaaagtg gcggtgtaat tcgtatgcca gaggccaacg atggaagaat
     2521 cgttgtcgtt tccatgattg cggtccgccc tcaccaactc gagttgtaaa cgcccggatg
     2581 acatgtggaa gacggccgga tttaccgctt tcagtgaggg agcgcattgc gccgcaacgc
     2641 ccgggactat gctaaagaag agtacgagta cactcacaaa tagcaaaagc ctgcaagaga
     2701 gcctacacat gatttttctc ctcgatttgg gttttgctga attgaacgca gcgaacgtgg
     2761 aacggatccg cgtttactct aggaggtaca tcacttccaa acaatagcct tttaggatac
     2821 tttttagggc acgacaatgg acgcgttcct ctcctggcga aagacgcggc cgagcgacct
     2881 ggcgcattgc ctagcgttgc acccggccaa gaacggcgcg gagattgtcg ggcgcgcccg
     2941 cgtcctagaa ggctggcgga aacttctgga gatgaggcac gccacgcgaa gcgccgtcgt
     3001 agaaatacac acgaaaggcc gggctgaact tgtcggcttt gggcttgcgt cgtttgtaaa
     3061 agaggatttt gttcgagacg aagtccagaa gccacgaccg gggctgaact cgaggctcat
     3121 cgacagcgtc gttaacggta atccagtggt cgcgacgtat gcggaagtgc gtgaagcaaa
     3181 cacgcgtggt gacctgcatc aggtgattct ggatacgagc tggaaaaacg gtcctctgac
     3241 tccagtgcag gtcgacgagg tgcgcgtact attaggccgc gcgtatcaag agctgttcgc
     3301 gggatatcat ttttccagga ttcttgtcga actggtggat gaactcgatt catggcatat
     3361 ccgtggcctc aaagtgtttc gaacgattga tgagttcgaa gacttttacc gcgacaatcc
     3421 ggcgaccaaa tggaatcgga atcggcgtct catcgacgtg acgctcgaca gcattcgcgc
     3481 cgacccacac tccgttgccg cggaactttt tcagcatcac ctgcggccgc aattagcgtt
     3541 gacgcgtggc gagcaagaat tgctcgagct ggcgctggac ggcgtagagg atgctgcagc
     3601 ggcggagtcg ctgtttgtga gcgtggccgc cgtcaagcgg cgctgggcaa gtctttttga
     3661 gcgcgtgggt gcgatcaggc ccgatctgtg cccgatggat ggtgagggta cgagaggaat
     3721 tcaaaagcgc cagcgcattt tgacgtacgt caggagccat cccgaggagc ttcggccgtt
     3781 caactccagc gcggccggat aggtcttcct ttcggagttg gtctcgttct cttggcgttt
     3841 cgcgatcagc cacccatcta ctgcggcaca tacaaatctt ttacaaagac aaatcttgac
     3901 aatccgtacc gcgttggtcc agtatcttct cagttgcaac agttttgcat ctatagcctg
     3961 gagatgctgg tggtattctt agcttactga attccttagc ttactgaata cacaaggctt
     4021 attcgtcgaa gagtgaatgg gcatggcgac aatgggatcg gcgattatgc cgcggacggg
     4081 tttgggacga cttgggctga ttatttgtgg aatcgcggca gtcgcagggg tgatggtttg
     4141 gcaggctgca acggcgtcgg gaagccctga tccgattgcg gcgcaaacga gcgcggcagc
     4201 ggtggtc
//
LOCUS       DATNDT010000276         4647 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4099078, whole genome shotgun sequence.
ACCESSION   DATNDT010000276 DATNDT010000000
VERSION     DATNDT010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4647)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4647)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4647
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4099078"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            316..612
                     /locus_tag="VN788_08960"
     CDS             316..612
                     /locus_tag="VN788_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20696.1"
                     /translation="MKRRRKTVGMVVFGALTLLSIVAVNSVPYEMRDKALLNICLGVI
                     AVVVLVVVYKRPLGSPDYDTESDYEDRSDLFLILLMACVYVGLWLFSSLFQHQK"
     gene            671..1765
                     /locus_tag="VN788_08965"
     CDS             671..1765
                     /locus_tag="VN788_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20697.1"
                     /translation="MLTIPQIEALRSTNPQLYESLKRLAGASLGPNQGWSLDSQITDG
                     DNYARVVAGALTSGKIDPTKSGMLMRGSLPPTWSGSFTYASTTSSLTWSWTGLTIFRA
                     DGATTPIADGSAAITGLAAGTTYYFYPYWDEAASALAWVAGGAGSSGFAQSAKTNSAA
                     QQQALQGRVPLSQGAIVAATTTSGTGGGSGGGSGSCLRAGSMVLTRERGTVPIESCKV
                     GEHLRCPSAAAGESWTRIIRLEVRDAETFIRLHFSNNESLDVTPHHIFTLADGSPMRA
                     ERLCLSDVFVGRFARLTLTRIEAISVASASRAVIPNPAAPFADEGEGSAVAFRDAPWQ
                     KVTVSCEPSHQFFAGRHSASILTHNYTFSS"
     gene            1824..2471
                     /locus_tag="VN788_08970"
     CDS             1824..2471
                     /locus_tag="VN788_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HXU20698.1"
                     /translation="MNPAYGRAVENALGDKTPSQSGDSTERTQPKGDGHWVTVNHRHI
                     LIDEDRNQTASRPRKRMSLSPQGLDFIKKHERFGSRIYLDITGRPTIGYGHLIKFGEN
                     FGEEITPKEAADLLRQDARRAEDEVNAALKVPVNQNQFDALTSIAFNAGPKAVAPENT
                     LLSKINQGRAVEQSDFTAYNKARMRNGSLVVSPGLTNRRLDEYDIFSTGNYKRSR"
     gene            2474..2947
                     /locus_tag="VN788_08975"
     CDS             2474..2947
                     /locus_tag="VN788_08975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20699.1"
                     /translation="MPRWAAILGAVTALAAIVFASNPPATLWGAWIVKKDLRVPGIVG
                     LSQKEIDQIVGTRILYSKSCAQSGQAVVRSPEYASTILSEREFFDYAHASLKQLGITG
                     TTTTKVTLTGSREPPLRFVGNIVFLGGKLPVIEVEGVFFELQKAKSIADNFDCTN"
     gene            3327..4043
                     /locus_tag="VN788_08980"
     CDS             3327..4043
                     /locus_tag="VN788_08980"
                     /inference="COORDINATES: protein motif:HMM:NF026945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymorphic toxin type 44 domain-containing
                     protein"
                     /protein_id="HXU20700.1"
                     /translation="MPGINGRFYANPLYGRALERARMAEAGHIWSEDYPELEPQYAWQ
                     QDLASDEHNAEAASQDRERRDGAHWVTIGHRHVLIDEAHASRAKQDQQPTKVCPSVPA
                     HPSDVNIDDNIRLVQKKLAKAKAADAAEGRSGRIGGNHAAVEAWFVNKVRPRGDWDYK
                     LHHPKGAYDAFGNFNFGATGSVLWNEDTLLRGAGALKALESGGKFGVPWKGPNYGNQS
                     DKNDEIRKGIQYYLNHCGDF"
     gene            4070..4582
                     /locus_tag="VN788_08985"
     CDS             4070..4582
                     /locus_tag="VN788_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20701.1"
                     /translation="MALVAIVAAPLCLGGMQASPPWPGSDISLRAQFLGHEADFETIV
                     KMAQQDIHVERIAQDFTWLDTDVSWPRKNVGITQPRWNRYRELFRRAGIPDGILNYAD
                     PPEVLFPIVSRGLVPAGQTKGIAYSPLPLAPVLASLDARPPARFYDNKGYVIAYKPIK
                     VHWYIYYEQW"
BASE COUNT         1043 a         1460 c         1185 g          959 t
ORIGIN      
        1 ttcgctgccg tttcatacga ctttctcctt aggggataga gtatcagata ttacggagga
       61 ccaaaaggag atagatcatc tcctagatta tatgcttttt gcatggtgtt tatttccaac
      121 ttccaatctc caacttccaa cctccaacgt ccaacctcca acttctgccc tatgctaacc
      181 acccccaaat cgaagcctac gaattgctga agcgaattgg cattcctcgt gctatatccc
      241 ggacatggcc aaaccgaggg aatcctcgca cttcctcccg ccgcttccga tgacgcgccg
      301 cgtcgggttt gcgccatgaa aaggcgtcgg aagacggtag gaatggtggt gtttggggcc
      361 ctaaccctgc tttcgatcgt tgctgttaac agcgttccct atgagatgcg ggacaaagcg
      421 cttctgaata tttgccttgg agtaatcgca gtggttgtac tcgtagtagt ctacaaacgc
      481 cccctcggaa gtccagatta cgacaccgag tccgactatg aagaccgctc cgaccttttc
      541 ctaatcctat tgatggcttg cgtgtacgtt ggcctttggc tgttctcctc cctgtttcaa
      601 caccagaagt gaacttttgc ccctagggtc cgctgccgtc tttgtctgca gtgcgagtat
      661 ccgaaatcac atgctaacca tcccccaaat cgaagccttg cgcagcacca atcctcaact
      721 ctacgaatcg ctaaaacgcc tcgcaggcgc ctcgctcggc cccaaccagg gctggagcct
      781 cgatagccaa atcaccgacg gcgacaatta cgcccgcgtc gtcgcaggcg cgctcacctc
      841 cggcaaaatc gatccaacca aatccggcat gctcatgcgc ggctcgctgc cgccaacatg
      901 gtccggctcc ttcacctacg cctcgaccac ctcgagcctc acttggtcgt ggacgggcct
      961 cacgattttt cgcgccgacg gcgccaccac ccccatcgcc gacggctccg ccgccatcac
     1021 cggactcgcc gccggcacca cctactattt ttatccttac tgggacgaag ccgcctccgc
     1081 gctcgcctgg gttgcgggag gcgcaggctc ctccggcttc gcgcagtccg cgaaaacaaa
     1141 ttccgccgcg cagcagcaag ctctccaagg acgcgtccct ctctcgcaag gcgcgatcgt
     1201 cgccgccacc actaccagcg gcacaggagg cggctctggc ggcggcagcg gaagctgttt
     1261 gcgcgcgggc agcatggtcc tcaccaggga acgcggcaca gtgccaattg aaagttgcaa
     1321 agtcggcgaa catctccgtt gcccctccgc cgccgcaggc gaatcgtgga cacgcatcat
     1381 ccgcctcgaa gtgcgcgacg ccgaaacctt catccgcctc catttctcca acaacgagtc
     1441 gctcgacgtc actccgcacc acattttcac tctcgccgac ggctctccca tgcgcgccga
     1501 gcgcctctgc ctaagcgacg tcttcgtagg ccgcttcgcc cgcctaaccc taacccgcat
     1561 cgaagccatt tccgtagctt cagcttctcg cgctgtcatc ccgaacccag ccgcgccttt
     1621 tgcggatgag ggtgagggat ctgctgtcgc tttcagggac gccccctggc aaaaagtcac
     1681 cgtaagctgc gaaccctcgc accaattctt cgccggacgc cactccgcct ccatcctcac
     1741 ccacaactac accttcagct cgtagctgat cggcgctttc atacagagac aaatcaaatg
     1801 cctcttatca acggcagttt tacgtgaatc cggcgtacgg tcgcgcggtc gaaaatgctc
     1861 tcggcgacaa aactccatcg caatcgggcg attcgaccga gcgcacgcag ccaaagggcg
     1921 acggccactg ggtcaccgtt aaccaccgcc acatcctcat cgacgaggac cgaaatcaaa
     1981 ccgcgagccg cccacgcaaa cggatgtcgc tcagccccca ggggctggat ttcatcaaga
     2041 aacatgaacg attcggcagc cgcatttatc tcgacattac ggggcgtccc acgattggtt
     2101 acggacacct catcaagttc ggcgagaact ttggcgagga aatcacccca aaagaagcag
     2161 ctgatcttct caggcaagat gcaagacgtg cggaggacga ggtaaacgca gcgctaaagg
     2221 ttccagtgaa tcagaatcaa ttcgacgccc tcaccagtat cgcgttcaac gccggaccga
     2281 aagccgtcgc tccagaaaac acgcttttga gcaaaatcaa ccaaggccga gccgtcgaac
     2341 aaagcgattt tacggcatat aataaagcga gaatgcggaa tggctcgctt gtggtcagtc
     2401 cgggcctcac aaatcggcgg ctcgatgaat atgacatatt ttcaacaggc aactataagc
     2461 ggagccgcta actatgccta ggtgggcagc gattcttggc gctgtaaccg cgctggcggc
     2521 catcgtcttc gcatcaaatc caccggccac tctgtggggt gcttggatcg taaagaagga
     2581 cttgcgcgtg cccggcatcg tgggcctgtc gcaaaaggaa atagatcaaa tcgttggcac
     2641 tcgcatcctc tattcaaaat cctgcgcaca gtctggccaa gctgtcgtga ggtcgcccga
     2701 atacgcctcc acgattctct ccgagcgaga attcttcgat tacgcgcacg cctccttaaa
     2761 acaattgggc atcacgggta caaccaccac gaaggttacc ctgactggct ccagagaacc
     2821 gcccttgcgt tttgtgggga atatcgtgtt cttaggggga aagcttcctg taatagaagt
     2881 cgaaggcgtt tttttcgaat tgcaaaaggc caagtcgatt gcagataatt tcgactgtac
     2941 caactgagcg ccactccata tgacgcccaa cacaaacgac ttttagcgcg atgtatgatg
     3001 tcgccccatg cccagccgtg acggtggaag cgcggggttt caaccccgcg aaaagtgcgc
     3061 ctcctcagga ggggcttcag ccccgggcct caacagccgt cgcgcccggc agctcttgta
     3121 gcgctggctt ccagccggcg aatttgcctt cgactccgga cgacgccccc tggccaaagg
     3181 tcatcgtaag ctgcgaaccc tcgcaccaat tcttcgccgg acgccactcc gccgccgtcc
     3241 tcacccacaa ctacactttt tcctcgtgaa cgcgattttg gattgacgat tgctccgcag
     3301 aatcgccgtc cacaaaaggg atcatcatgc ctgggataaa tggcaggttt tacgcgaatc
     3361 cgctctacgg tcgggcgctc gagcgggcaa ggatggccga agctggccac atttggtcag
     3421 aagactatcc cgaattggaa ccgcagtacg cctggcagca agaccttgcc agcgacgagc
     3481 acaatgctga agccgcttcg caagatcgcg aacggcgaga cggcgcgcac tgggtcacga
     3541 tcggccaccg tcatgtcttg atcgatgagg cgcacgccag ccgggcaaag caagatcagc
     3601 agcccacgaa ggtctgtccg tcggtcccag cccacccatc cgacgtcaat attgatgata
     3661 atattcgtct cgtccaaaag aagctggcga aagccaaagc tgccgatgca gcggaaggcc
     3721 gtagcggacg tatcggcgga aatcacgctg cagtagaagc gtggttcgta aacaaagttc
     3781 gccctcgcgg cgattgggat tacaagctac atcatcctaa gggtgcatac gacgcctttg
     3841 gcaacttcaa cttcggggct acgggttcgg ttctgtggaa cgaggacacc ttgctccgag
     3901 gggctggcgc gctgaaagct ttggaatccg gcggaaaatt cggcgtgccg tggaaagggc
     3961 cgaattatgg gaatcaatcg gataagaacg acgagattcg aaagggaatt cagtactacc
     4021 taaaccattg cggcgatttc tgaaaacaat tcgtgagcga gtcgtagaga tggcgctagt
     4081 cgcgatcgtc gccgcgccgc tctgcctcgg cggcatgcaa gcttcgcctc cgtggcctgg
     4141 atccgatatc tctctgagag ctcagttcct cgggcacgaa gcggactttg aaaccatcgt
     4201 caaaatggct cagcaagata ttcacgtaga gcgaatcgcg caggatttta cgtggctcga
     4261 cacggatgtc tcctggccta gaaagaatgt tggaattact cagccaaggt ggaataggta
     4321 tcgcgaactc tttcgccgtg ccgggattcc cgacggcatc ctcaactacg ctgatccacc
     4381 tgaggttttg ttccccatcg tctcccgagg actggtcccc gccggccaaa cgaaagggat
     4441 cgcctacagc cccctgccct tggcccccgt actcgcgagc ctggacgcca gaccgcccgc
     4501 gcgcttctac gacaacaaag gttacgtcat cgcctacaag cccatcaaag tccattggta
     4561 catctattac gagcaatggt gactcctaaa ttgtttcttg tagcatcacc gccgtttctc
     4621 tgattttcca ttttcgcttt tcgcgtg
//
LOCUS       DATNDT010000277         5324 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_393176, whole genome shotgun sequence.
ACCESSION   DATNDT010000277 DATNDT010000000
VERSION     DATNDT010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5324
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_393176"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(345..1160)
                     /locus_tag="VN788_08990"
     CDS             complement(345..1160)
                     /locus_tag="VN788_08990"
                     /inference="COORDINATES: protein motif:HMM:NF023580.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HXU20702.1"
                     /translation="MSMNAIPVAIFMFGALGAFAYWGMKFSRAPELMMFILFPAAFIP
                     LNYFWLKRKLVKLKSMQFPEGVPHTPSNFTYAANVEGSSGAAPAYSPNAQYAALAALP
                     APRQIRLAKSSTWFRWVVAFSLAPIVVVIATNLFQDWARTSSLAGMGRRDLIMAAAAG
                     GVLMLAAGIWRSQMRECDLLEHGEVVMGCVVRQWSDQKQGSSVEYEFTDFLGQTHRAV
                     GFDRTMRLYSGMPVPVFYDRDNPKRQIAYCAALHEVILPAAVEPRSEELITKP"
     gene            complement(1390..2055)
                     /locus_tag="VN788_08995"
     CDS             complement(1390..2055)
                     /locus_tag="VN788_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HXU20703.1"
                     /translation="MALLERVSTLIRANLNDLVDKAENPEKVIKQVILDMENQYLQVK
                     TQVAIAIADLHMLQKKKDEYQESMGEWMRKAEKSVAIQQDELARAALERYRNFRQMAQ
                     SYNEQVDDQQSQVEGLKNALQKLEQKLVQAKAKKDLLLARHRRTQVAAKANQAGAAIG
                     AGSKAAAFDRMSEKVLRAEAVGEAHKQLTGDNVEEQFAELEKNEEVERLLAEIKGRKA
                     LNP"
     gene            complement(2140..3582)
                     /locus_tag="VN788_09000"
     CDS             complement(2140..3582)
                     /locus_tag="VN788_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HXU20704.1"
                     /translation="MDVWVIAGLMVLVLVFLMAMFASMFRKAGPHEALIVYGFRGTRI
                     VKGGGTIIFPMVENFRELSLELMSFDVAPQQDLYTKQGVAVTVEAVAQIKVKSDPESI
                     RTAAEQFLTKTPVLREGLIRLVMEGHLRGIIGQLTVEQIVKEPEMVGDRMRSTCADDM
                     NKMGLEVISFTIKEVRDKNEYITNMGRPDVARIRRDADVATVEAERDTAIKRAEAKRE
                     SAIAEAQADQERVFAETSSLAKQAEATRDLEVKKAAFLEDTKKAQAQADKAYELETNK
                     MQQQVVAEAVRVQQVERENQIKVQEAEIVRHEKELIATVLKGAEIERQRIETLANAEK
                     SKRIAEADGAACAIRAQGEAEAEIVFKKGEAEAKAMNVKAEAYQEYNQAAVIDQLFTT
                     MPEIVKAIAAPLANVDKVTIVSTGNGDGAGAYKLTGDVTKIAAQIPALFEALSGMQMS
                     ELLSKVKLIGDKPARPEIPGATTPGSARQI"
     gene            complement(3614..4258)
                     /locus_tag="VN788_09005"
     CDS             complement(3614..4258)
                     /locus_tag="VN788_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20705.1"
                     /translation="MTWAGFYLVCFVVGFALSFFSFAFGALHLHLPTKWHLGHATHGH
                     GVHGAHAGHGAHGAKSAASKAMSWFTPPAIFAFLAWFGGTGFLLTQYYGGWFLLVLTI
                     AVLAGCVGEGIVWWFLVKTLLPHETVLHESDYELVGTIARVNSPIREGGTGEIVFSQA
                     GVRRCAGARRDNGKALEKGREVVIARVDRGIAYVAPWEEWAKLQTVDRNSQEPE"
     gene            complement(4276..4812)
                     /locus_tag="VN788_09010"
     CDS             complement(4276..4812)
                     /locus_tag="VN788_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU20706.1"
                     /translation="MTWYIAICDMYIAHCDMTLGEKIRHLRAVEGTLRGLGRPLTQGE
                     VIGQMKRELRRTISQSYLSQIESGARPHMTHGTRDLLARFFKVHPGFLVDDAEGYHPE
                     LRSDLRIKDASIDSWLYAAAETFHDDSRFAEALRLIAEFPDTRKALLLLGEAVKVPGL
                     IDHLYDTLKSGPRQLVRT"
     gene            complement(4924..>5324)
                     /locus_tag="VN788_09015"
     CDS             complement(4924..>5324)
                     /locus_tag="VN788_09015"
                     /inference="COORDINATES: protein motif:HMM:NF044456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20707.1"
                     /translation="EYMIGDRMLVAPLFAGEPSRKVTLPAGKWHDFWTGAEVQGRTFS
                     VPASTDKIPVYVKAGSVLPLAGVSASTADSGARELTVMIFGDGSLPFSMATPSGETLE
                     LRWDPSKSAGNVRQAATDKPYNIVSWKRTS"
BASE COUNT         1067 a         1632 c         1378 g         1247 t
ORIGIN      
        1 gtttgtcagc gtgttgtgaa gcttgatcgg gatcatcgtc tagccgaagc cgggtgcgtt
       61 agtcgtcctc cgccgcgcaa aggctcacca ttctagccga caggcggcgt tcctcgcaaa
      121 cgtcggcttc tatgtctcag cgccgtcctc ctgtcgtaaa agtcggctca aaaattaagt
      181 tttttgtcga ttagaaattg agttttttgc gtaactctac gctgcgagca gcccgaacag
      241 cttagaatcg tgtaatcgaa taccagtctt gcccgcacac gtgaggagaa aaaatagggc
      301 aggtcaggct atcccggatc tccaagggcg ttgctgcggc gggtttatgg cttggtgatc
      361 agttcttcgc ttctcggctc aactgctgcg ggaaggatca cttcgtggag ggcggcgcaa
      421 taggcgatct gtctctttgg gttgtcgcgg tcgtagaaca cgggcacggg catgccggaa
      481 tagagtctca tcgtacgatc gaaccctacc gcccggtgcg tttggccgag aaaatccgtg
      541 aactcgtact cgaccgaaga tccctgcttc tgatcgctcc actggcgcac cacacatccc
      601 atcacaactt caccatgttc aagcagatcg cattcgcgca tttgcgagcg ccagatgccg
      661 gctgcaagca tgagaactcc tcctgcggca gcggccataa tcaaatccct tcgtcccatg
      721 ccggcgagag atgatgttct tgcccagtct tgaaacaaat ttgtagctat gacgacaaca
      781 ataggagcga gcgaaaaggc gacaacccat ctgaaccagg tgctgctttt tgcgaggcga
      841 atctgccgcg gcgccggcaa agccgccagt gccgcatact gcgcgttcgg tgaatatgcg
      901 ggcgccgctc ccgaggaacc ctccacattc gcggcatacg tgaaattcga tggggtgtga
      961 ggaacaccct cgggaaattg catggatttc agctttacca gctttcgctt caaccagaaa
     1021 tagttcagcg ggatgaacgc agcggggaac aatataaaca tcatcaactc gggtgcgcgg
     1081 ctgaacttca ttccccaata tgcgaaagcg cccagcgcgc cgaacatgaa tatcgcgacg
     1141 ggtatcgcgt tcatgctcat ctttacactc gatatcgccg cagtccggtt ccagccgcaa
     1201 tgcggacaaa agaccgcgct gttggcaaag gctgtcttct gatggcagac ggggcactcg
     1261 cgcaggacca ttcaacaagt ctagcttgca aaaactcgct ctcgcccggt tcgcaagcgg
     1321 agttgctatc ccagcaaaaa gtagcgaggc gaagagaagg gaaacgcgct gcgttcgccg
     1381 gtgccttctt taaggattga gggctttgcg acctttaatc tcagcgagca ggcgctcgac
     1441 ctcttcgttt ttttcgagtt cggcgaactg ctcctcgacg ttatcgccgg tgagctgctt
     1501 gtgagcttcg ccgaccgctt cggcgcgcaa caccttttcg ctcatccggt caaacgccgc
     1561 cgctttcgag ccggccccga tggccgctcc cgcttgattc gcttttgcgg ccacctgcgt
     1621 gcgccggtgg cgcgcaagca ataagtcctt cttcgctttg gcctgcacca gcttttgttc
     1681 gagcttttgc aacgcgttct tcagcccttc gacctgagac tgctgatcgt ctacctgttc
     1741 gttatagctc tgagccattt gccgaaaatt gcgatagcgc tccaatgctg cgcgagccag
     1801 ctcgtcctgt tggatggcga cggacttctc ggccttgcgc atccattcgc ccatcgattc
     1861 ctgatattcg tccttcttct tttgcagcat gtgcaaatcg gcgatggcaa tggccacctg
     1921 cgtcttcact tgcagatact gattctccat atcgagaatc acctgcttga tcaccttttc
     1981 ggggttttcc gctttatcta ccaggtcatt caaattcgct cggattaacg tgcttactcg
     2041 ctccaaaagt gccatggaaa cctccagatc ctcacttcac aaaacgcgtt aacaaacgaa
     2101 gtggccgtgc ccgccttgcg gcgggcacag cgcccgcatt tatatctgac gcgccgatcc
     2161 tggtgtcgtt gcgccgggaa tttcgggacg tgcgggctta tcgccgatca gcttcacttt
     2221 cgacagcagc tcggacattt gcatacccga aagcgcctca aagagcgcgg ggatttgcgc
     2281 cgcgatcttc gttacgtcac cggttagctt gtaagcgcct gctccatcgc cattgccggt
     2341 cgaaacgatc gtgaccttat cgacgtttgc cagcggcgcg gcaatcgcct tcacaatttc
     2401 aggcatggtt gtgaacaact gatcgatcac ggcagcttgg ttatattcct ggtaagcctc
     2461 ggccttcacg ttcatggctt tggcttctgc ctcgcccttt ttgaagacga tctcggcctc
     2521 ggcttcgcct tgtgcgcgaa tcgcgcacgc cgcgccatcc gcctcggcaa tcctcttcga
     2581 tttttcggcg ttcgctaggg tttcaatgcg ttgccgttcg atctcggcgc cctttagcac
     2641 cgtcgcgatc agctcttttt cgtggcggac aatctcggcc tcctgcactt tgatctggtt
     2701 ctcgcgctcg acttgctgca cgcgaaccgc ttctgccacg acctgctgct gcattttgtt
     2761 tgtttcgagt tcataagcct tatcggcctg cgcctgcgct ttcttcgtgt cctccaggaa
     2821 tgcggccttc ttcacttcga gatcgcgcgt ggcttctgct tgctttgcga gagacgatgt
     2881 ctccgcgaaa actcgctcct gatcggcttg cgcctccgcg atagccgatt cgcgcttcgc
     2941 ttcggcgcgt ttgatcgcgg tgtcgcgctc ggcctcgact gtcgctacat cggcgtcacg
     3001 gcgaatccgc gctacgtccg ggcggcccat gttcgtgatg tactcgttct tgtcgcgcac
     3061 ttctttgatc gtgaaggaga ttacttcgag acccatcttg ttcatgtcgt cggcacaggt
     3121 ggagcgcatg cggtcgccta ccatttccgg ctctttcacg atctgctcca cggtcagttg
     3181 tccgatgatg ccgcgcaagt ggccctccat cactaatcga atgagccctt cgcgcagtac
     3241 tggtgttttt gtcaggaact gttcggccgc cgtgcgaatc gattccgggt ctgacttcac
     3301 cttgatctgt gctacggctt cgaccgtcac ggcgacgcct tgctttgtgt aaaggtcctg
     3361 ttgcggcgct acgtcgaatg acatcagctc cagcgagagc tccctgaaat tttcgaccat
     3421 cggaaaaatg atcgtcccgc cgcccttcac gatgcgcgtt cctcggaacc catacacaat
     3481 cagcgcctca tgaggtcctg ccttgcgaaa catcgaggca aacatcgcca tcaggaaaac
     3541 cagcaccagc accattaatc ccgcaatcac ccaaacatcc atcccgatct gaggcacgtt
     3601 cgttctcctt cctttactcc ggctcttgcg aattcctgtc gactgtctgc aacttggccc
     3661 attcttccca tggcgcaacg tatgcaatac ccctgtctac gcgtgcgatg accacttctc
     3721 gccccttctc cagagccttc ccgttatccc gccgggcgcc tgcgcaccgc cttacgcccg
     3781 cctgcgaaaa aacgatctct cccgttccgc cttcgcgtat gggactgttc acccgcgcga
     3841 tggttcccac gagttcgtaa tcggactcgt gcaacacggt ttcgtgcggc aaaagggttt
     3901 tcaccagaaa ccaccagaca atcccttcgc caacgcaacc cgcgagaacg gcaatcgtaa
     3961 gcacgagcag aaaccagccg ccgtaatact gcgtgagtag aaacccggtg ccgccaaacc
     4021 atgcgaggaa cgcaaaaatt gcgggcggcg taaaccacga cattgccttg ctcgctgcac
     4081 ttttcgcccc atgggccccg tggccggcat gagcgccatg cacgccatgg ccgtgtgtcg
     4141 cgtggcccag atgccacttt gtgggcagat gcaaatgcag cgcgccgaaa gcgaatgaga
     4201 aaaaactaag cgcaaaacca accacgaagc agaccaggta aaaaccagcc caagtcatcg
     4261 ccacctcccg cgacatcatg tccgcaccaa ttgccgcgga ccgctcttca acgtgtcata
     4321 caaatgatcg atcagtcccg gaactttgac cgcttcgccc agcagcaata gcgcttttcg
     4381 cgtgtccgga aactccgcga tcaagcgcaa tgcttccgcg aaccgtgaat cgtcatgaaa
     4441 tgtctcggcc gccgcataga gccacgaatc gatgctcgca tctttaatgc gaagatcaga
     4501 ccgaagctcc gggtgatagc cttcggcgtc gtccaccaaa aatcccggat gcactttgaa
     4561 aaatctcgcc agcaaatcgc gagttccgtg cgtcatgtgt ggccgcgcgc cactctcaat
     4621 ctgcgagaga taagactggc tgatcgtccg ccggagttct cgcttcatct ggccgatcac
     4681 ttcgccttgg gtgagagggc ggccgagtcc gcgcagggta ccttcgaccg ctcggaggtg
     4741 ccggattttt tcgcccagtg tcatatcgca atgtgcgata tacatatcgc agattgcaat
     4801 ataccaagtc aagaggaaat atcgcgcgtg aaatcaggca cttaggatat ggttgcgcta
     4861 ttctttcgcc catagatggg gcgatctaag cgcgcgattg tggaaattgc tctatcgaac
     4921 cggtcagctt gttcgtttcc aactcacaat gttgtacggc ttgtcggtgg cggcctgtct
     4981 cacattgcct gcggattttg aaggatccca gcgcagttcc agcgtttctc cgctgggcgt
     5041 cgccattgaa aagggcagcg agccatcgcc aaaaatcatc accgtcaact cgcgagcgcc
     5101 ggaatccgcc gttgaagcgg acacgccggc aagcggcagc accgatcctg ccttcacgta
     5161 cacgggaatt ttgtccgtac ttgccggcac gctgaacgtt cggccttgta cctccgctcc
     5221 agtccagaaa tcgtgccact tgccggcggg aagcgtcact ttgcgcgaag gctcgcccgc
     5281 gaaaagcggc gcaaccagca tgcggtcgcc gatcatatat tcgt
//
LOCUS       DATNDT010000278         5934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1741705, whole genome shotgun sequence.
ACCESSION   DATNDT010000278 DATNDT010000000
VERSION     DATNDT010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1741705"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..222
                     /locus_tag="VN788_09020"
     CDS             <1..222
                     /locus_tag="VN788_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU20708.1"
                     /translation="GLLENLANKEVADRLHISERTVKFHVSNLLAKFGVRRRADLILL
                     CYQRRAVAAPVTVSANGYSQEPVISSVRK"
     gene            complement(347..760)
                     /locus_tag="VN788_09025"
     CDS             complement(347..760)
                     /locus_tag="VN788_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20709.1"
                     /translation="MTALQSARTRRTISPILALGEWLMILPASVFLAVAALRQMQPAK
                     FEPAHTSWTIFSWAVSHVTHTDAAVIFLALPAIVLLAGCTHLLRVWSGSEIFRQDLHD
                     AFGILRRNFAACAMASAAALGGVILAAVIAHLIVG"
     gene            complement(871..2327)
                     /locus_tag="VN788_09030"
                     /pseudo
     CDS             complement(871..2327)
                     /locus_tag="VN788_09030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009067938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
     gene            complement(2570..4807)
                     /locus_tag="VN788_09035"
     CDS             complement(2570..4807)
                     /locus_tag="VN788_09035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HXU20710.1"
                     /translation="MRKKSFVALAGAFFLLGAGASLSQQPWQHMQNLTAADIAAQWKS
                     PQPEYGPEPYFGLNGPVSIAEVDRDLDTLHGLGFQAVTVQAGFGTPFAYLSPQYFDFF
                     RQFVQEAKKRDMRVWIVDDAGYPSGFAGGKFTTEKPNLRMQALEVAQKLSVAGGETLH
                     RDLPADTISAAAVNQDGTAVPITLHGNTLDWTAPKTPNGTWTVLAVDHEFRTSPTRSD
                     TNPHRVKDTEQSLEDYMDPAATHQFLAFTHEQYKQAVGDEFGTTIMGFRGDEPDYSIS
                     GLPWTPAFFARFRELKGYDVQPYTALFAQTPSRNNPGVTIHLTPEQLRIKGDYYDVFS
                     RMIVAGFFDPQGKWAAANQLEYQVHLNHEEMEMELTHSEGSFFRDMRWVEVPGIDAIW
                     HQIWTDTVSDYPRLASSVSHVYGKPRAFTESFAAYTPEPDVKMARYILNEQFVRGVNL
                     IEMMYFPSTSTGPRPLSGYMGDPGFPDLVTYVRRMSYLMAMGRPDASVALYLPSSSMW
                     LGDRDSDTQFVSTERLLSEHQIDFDIVSDDALARDLKAGKGSFETLSGNRYRTVILPD
                     PLVISSQAFARLKAFAQGGGKVLFIGGTPKWIAAQTIRDAKAATPSDFFWAKIVDAQL
                     PPTPTPPQFPPTAPLQPQVVPDAILAALQAAVPEATVRLDSADPALKVMKRRWADADV
                     YLLFNEGQQPSEHTVTLSSDGKTVESWDAQTAAIEPINVTRQAGDLAVHVKLDPYATS
                     VLVIR"
     gene            complement(4932..>5934)
                     /gene="dnaB"
                     /locus_tag="VN788_09040"
     CDS             complement(4932..>5934)
                     /gene="dnaB"
                     /locus_tag="VN788_09040"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HXU20711.1"
                     /translation="ASAIQEQALAAGDDADIILDRAESAIFQLAEDRVRAGLIGVKEL
                     VRDGFERLEKIFSEGRRITGLPTGYSLLDNETAGLQPSELIILAARPSMGKTAFALNI
                     AENIAVRNREPVAIFSLEMSKESLLLRLLASQSRVDAHKFRTGHMNRDDWSKITSSLA
                     ELGEAPIWIDDSASSTVLEMGAKARRLKRDRGLSLMIVDYLQLVTPTSTKRASNRQEE
                     VSGMSRALKGLAKELKIPIVVLSQLTRAPEREERKPQLADLRESGAIEQDADVVLFIN
                     RPNFYKTDLPDEERAKYELIIAKQRNGPTGTMNFVFLSRHTRFEEAAPDAWNSGMDE"
BASE COUNT         1191 a         1599 c         1854 g         1290 t
ORIGIN      
        1 ggccttctgg aaaaccttgc taacaaagag gttgccgaca gacttcacat ctccgaacgc
       61 actgtgaaat tccacgtttc gaatctgctt gccaagttcg gcgttcgccg tcgtgcagac
      121 ctcatcctgc tctgctatca aaggcgcgcc gtggctgcgc ccgtcaccgt tagcgcgaac
      181 ggctatagcc aggagcccgt catttcgtca gtgcggaagt aacaattttg gcgcgaatgg
      241 cgccttaaaa ttgccttaaa tggtcttaaa gggccaagaa tggcgcgttt gacaccctaa
      301 ttttcgatcc gccgtgaaca cccgctgtta ccctccgatt tgaagactag cctacgatca
      361 aatgcgcgat caccgcggcg aggatcacgc cgcccagcgc cgccgccgat gccatcgcgc
      421 acgcggcaaa atttcgacgc agaattccga acgcatcgtg caaatcctgg cgaaatatct
      481 cgctgccaga ccagacgcgc aggaggtgag tgcatcctgc gagcagaaca atcgcgggca
      541 aggcgagaaa aatgacggcg gcgtccgtgt gggtgacgtg ggaaaccgcc cagctaaata
      601 ttgtccaact agtgtgagcc ggttcaaatt ttgcgggttg catctggcga agcgccgcga
      661 cggccagaaa gaccgacgcg ggaagaatca tgagccattc gcccagggca agaatcggtg
      721 aaatggtcct gcgggtccgc gcactttgca gagcggtcat ttaggtcctc ctgtggagta
      781 ctttatattg caaagtacat gtacgtgtca agcgggatct tggctctttc ccccgaatga
      841 catgtagtta aaacgtgtga acctcagggc tcacgtctga gggcgcttct tctcctgctg
      901 gggcggcttc tggtccgcgc cgggcagctt tggtcttggg agcttcttgg tttcgtacat
      961 cgctctttgc aaagcaccgc ttgtatcggg cagcacaggt ggcttaccgg gagcttgctt
     1021 gagacgcaga gccgaggtga gatcgccgaa ggtgcttcgg cgccaatcag tgatattcgg
     1081 ttcgcgcacg cccgtgaact gctcgaggaa gcgcaacgta gaggtgtgat cgaagggctc
     1141 gcttgaaacc cagccgccgg tcgtccacgg cgaaatcagg atacacggca cgcgaaagcc
     1201 cgcgccgatc ggcagcccac ctataaactc gtgcgcagtt ccggcgggag gcgttggcgg
     1261 cacgacgtgg tcgaaaattc cgtcgttctc gtcataattc aaaatgaaga gcgtcttcgc
     1321 ccaaacgtcg gggttcgcgg cgatcgcatc aattttgctc gctacgaagg ccgcgccatc
     1381 cgcaggcatg tagtcaggat gttcggattg atagtccgta cagatgaccc atgagaccgc
     1441 cggcaatttg tcgttaattg cgtcgtactc gaattctcct tcggatccgt acgccacgcc
     1501 tttgaggaac aatggagaac ggcgcgcggc attttggaaa acggcgaagt tttccagcat
     1561 gttgcagcca tagttgttct ccatctgata gaccttccac ggcacgccgg cttcttcgag
     1621 tctctcggga tatgttttcc atgtatagcc gttgggtgtc gctgtgttgc tgatcatggg
     1681 gccgccgcca gtcgcatcgg gatcgatcat gccggtcatc cagtacatgc gattgggcca
     1741 cgtgggaccc atgacggagc agtggtagtt gtcgcagagc gtgaagctct cggcgagcgc
     1801 gaactggaag ggaatgtctt cgcgcgtgta gtagcccatg caataggggc cgttacggcc
     1861 atcggctttg cgatgcgctt gcagccactg atccattttg ccgccgttcc acgcttcatg
     1921 ctgcacggcc catgcgtggc ttgtagaggg aattttctgc gcgctggttg cgcgtgtatc
     1981 cagatggaac gggagcaggt aaccttcggg gttctccgcg tccggctgat aaaagacgga
     2041 gcggccgcca tcgagcttga gcgcctttgg atcgtcaaac ccacgaactc cggcgagcgt
     2101 gccgaaataa tgatcgaacg accggttttc ctgcatgagc agcacgacgt gtttgatatc
     2161 cgccagcgag ctattctttg gcgcgccttg cgcgagcatg cggcgcacgt ttggcggcat
     2221 gagcgaacct gccgcggcgg cagcggcgac ctgcgcggct ccttcaatag acgccgacgc
     2281 gtgagattcg cgccaaacag gctgtgatcg attgagtcgt gcttcatgtt ttcaaccccc
     2341 tggaagaatt cccgctatga cggcgcaaag atacctcatt gcgtggcgga taggctggaa
     2401 ttttttatta aacatttgtg acacgcgggc gagcacccaa gcgggagtcg ggggaattcg
     2461 catggattaa cgagcgaaag caagagggat cctttccgcc acggcggacc tagggcgccg
     2521 cgcccttaag acgggcgcgg ctcaggatga cggattaaag acacgggttt cagcggatga
     2581 cgagcacgct tgtcgcgtaa ggatcgagtt tgacgtgcac agcgagatca ccggcctggc
     2641 gcgtgacgtt tattggctct atggctgctg tttgtgcatc ccaagactcc accgtttttc
     2701 catcgctcga gagcgttact gtgtgctcgc tgggctgctg gccttcgttg aacagcaggt
     2761 agacgtcggc gtcggcccaa cggcgcttca ttactttcag cgcaggatcg gcactgtcga
     2821 ggcgcactgt ggcttcagga acggctgctt gaagcgccgc gaggattgcg tcgggtacga
     2881 cttgcggctg caacggcgcc gttggcggaa attgcggcgg cgtgggcgtg ggcggcaatt
     2941 gagcgtccac gatttttgcc cagaagaagt cggagggagt ggcggctttt gcgtcacgga
     3001 tagtttgcgc ggcaatccat ttgggtgtgc cgccaatgaa gagcactttg ccgccgcctt
     3061 gcgcgaatgc tttgagccgg gcgaaggctt gcgatgagat cacgagcgga tcgggcagga
     3121 tcaccgtgcg gtaccgattg ccgcttagag tttcgaagct gcctttgcct gccttcaggt
     3181 cgcgggcgag cgcatcgtcg ctgacgatat caaagtcgat ctgatgctca ctcaagaggc
     3241 gttcggtgga gacaaattgc gtatccgagt cacggtcgcc gagccacatc gagcttgacg
     3301 gcaggtaaag tgcgacggac gcatcgggac ggcccatggc catgaggtag ctcatgcggc
     3361 gcacatacgt gaccagatcg gggaagccgg gatcgcccat gtaaccggaa agagggcgcg
     3421 ggccggtgga tgtggaaggg aagtacatca tctcgattag attgacgccg cggacgaact
     3481 gctcgttgag aatgtagcgc gccattttca cgtccggctc gggagtgtat gccgcaaaac
     3541 tttcggtgaa agcgcgaggc ttgccgtaaa cgtgagagac ggaactcgcg aggcgcggat
     3601 aatcggaaac ggtgtccgtc cagatctggt gccaaatggc atcgatgccc gggacttcaa
     3661 cccagcgcat gtctcggaag aatgaaccct cgctgtgggt caactccatt tccatctctt
     3721 cgtgattgag gtggacctgg tattcaagct ggttggcggc ggcccatttt ccttgcggat
     3781 cgaaaaagcc ggcgacgatc atcctcgaaa aaacgtcgta gtaatcgcct ttgatgcgga
     3841 gctgctcggg cgtgaggtgg atcgttacgc ccggattgtt gcgcgatggc gtttgcgcga
     3901 aaagcgcggt gtagggttgg acgtcatatc ccttcaattc gcgaaagcgc gcgaagaagg
     3961 cgggcgtcca aggaaggccg gaaatggaat agtccggctc gtcgccgcga aagcccatga
     4021 ttgtcgttcc aaattcgtcg cccactgcct gcttgtactg ctcgtgcgtg aacgcaagaa
     4081 actggtgcgt ggcggcggga tccatgtagt cttcgagcga ttgctcggtg tctttcacgc
     4141 gatgcggatt tgtgtccgaa cgagtgggag aggtgcggaa ttcgtggtcg acggcaagga
     4201 ctgtccacgt tccgttcggc gttttgggcg ccgtccaatc gagcgtattg ccatgcagcg
     4261 ttatcggaac agccgtgccg tcttgattga cggcggcggc gctgatggta tcggcaggga
     4321 ggtcgcggtg cagggtctcg cccccagcga cggataactt ttgcgctact tcaagtgcct
     4381 gcatgcggag attcggcttt tccgtggtga atttgccgcc ggcgaagccg ctcggataac
     4441 cggcatcgtc gacgatccac acgcgcatat cgcgtttttt cgcctcctgg acgaattggc
     4501 ggaaaaagtc gaaatactgc ggcgagaggt aagcgaacgg cgttccaaat cctgcctgca
     4561 cggtaacggc ttgaaagccg aggccgtgca gcgtgtcgag gtcgcgatca acttccgcga
     4621 tgctgactgg gccattcaag ccgaagtatg gctcgggtcc atactcgggc tgcggagact
     4681 tccactgtgc ggcgatatcg gctgcggtga gattttgcat gtgctgccaa ggttgttgcg
     4741 agaggctcgc tccggcgccg agtagaaaga acgcgcccgc tagggcaacg aaggattttt
     4801 tgcgcatgaa tactcctttg ccggattacc tagatggttc gacaaagaga gcgcaacgat
     4861 acaacatgag ggaccgctcc ggcgcgaatt ttttgtcagt taaatttgtg aaagccaatg
     4921 gccgagtaaa actattcgtc catgccggaa ttccatgcgt cgggcgcggc ttcctcgaat
     4981 cgagtgtggc ggctcaggaa gacgaaattc atggtgcctg tgggaccgtt gcgctgtttg
     5041 gcgatgatca actcgtattt cgcgcgctct tcgtcgggaa gatcggtctt atagaaattc
     5101 ggacgattga tgaagagaac gacgtcagcg tcttgctcga tggcgccgga ttcgcggagg
     5161 tcggcgagct gcggcttgcg ctcctcgcgt tcgggcgcgc gtgtgagctg gctaagcacg
     5221 acgattggta tcttcaactc tttcgccagg cccttcaggg cgcgcgacat gccggagact
     5281 tcttcttgcc tgttcgaggc acgctttgtg ctggttggcg tcacaagctg gagatagtcg
     5341 acgatcatca gcgaaagacc gcggtcgcgt tttaatcgtc gcgctttcgc gcccatttcc
     5401 agcaccgtgg aggaagcgga atcgtcgatc cagatgggcg cttcgccaag ctccgccagc
     5461 gaagaggtga ttttgctcca atcgtcgcga ttcatgtggc cggtgcgaaa cttgtgcgca
     5521 tccacgcgcg attgcgacgc gagcaagcgc aggagcagcg attctttcga catttccagg
     5581 ctgaagatgg ctaccggctc gcgatttcga accgcgatgt tttccgcgat attcagcgcg
     5641 aacgccgttt tccccatcga aggccgcgcg gccaaaatga tcagctcaga gggttgcagg
     5701 ccggcggttt cattgtcgag caggctgtag cccgtgggaa ggcccgtgat gcggcggccc
     5761 tcgctgaaga ttttttcaag gcgctcgaag ccatcgcgaa cgagttcttt gacaccgatg
     5821 aggccggcgc gcacgcggtc ttcggcaagc tgaaaaatcg cggattcggc gcgatccaga
     5881 atgatgtccg catcgtcgcc cgcggcaagg gcttgctcct gaattgcgga tgcc
//
LOCUS       DATNDT010000279         8167 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3214805, whole genome shotgun sequence.
ACCESSION   DATNDT010000279 DATNDT010000000
VERSION     DATNDT010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8167
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3214805"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            682..1203
                     /locus_tag="VN788_09045"
     CDS             682..1203
                     /locus_tag="VN788_09045"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXU20712.1"
                     /translation="MSFPHKNIRLHRFSYSGQSSYFVTLCCAGKRPVFANTKNAVWLV
                     EILRKHSNAHRFAIYAYCVMPDHFHVLVFGLDPTSSLLAFVKNLKLTTSRGYMKDFGR
                     VLWQKKFYDHILRPRDNPAAVAAYIWMNPVRKELCKDPRQYPYSGSFVMDWKKAISHV
                     ESWVPDWKEKAPA"
     gene            1322..2197
                     /locus_tag="VN788_09050"
     CDS             1322..2197
                     /locus_tag="VN788_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20713.1"
                     /translation="MSTLSARRALRVAGIGAVAVLALTWIPRANGQLPGGQMGASVPN
                     QTQIGQSGGTMNDDLQYGTFGHGVEREQANAYQVFLRERDPARKIRLGNDFLKKYPKS
                     ALAERVDAELMNVYRAKQDWKNVYLCADHALALQPDDVDVLTTVGWTIPHVYNPSDPD
                     ADQELKTAETYAKHAIEVLAKMPKPAGLTDEQFAAAKAARTFQAHSALGLVYFRRQDF
                     SDSARELAESTKGNPRQDPTDLYVLGVDLQNLNRHSEAAAAFRGCSQLEGVLQNQCKQ
                     SAQVAQAQADKSKAR"
     gene            2412..3719
                     /locus_tag="VN788_09055"
     CDS             2412..3719
                     /locus_tag="VN788_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HXU20714.1"
                     /translation="MCAPLLLSSPLQLAGLLQGNQAPQAGQGAGTSPFYQIDEAFLEA
                     VENAHFQFFWEQANPDTGIVRDRCNAIAPDKSDLGSIASLGFGLTALCIGDKRGYLPH
                     NEARGRALNSLRFLWKKLPNHRGFFYHWANVNTGERLWDSEVSSIDTAILLCGVLTCG
                     AYFEHSEISELASMIFNRVDWSWLSEDTTLLPHGWRPETGFLQYRWDDYSEMMMMYLL
                     GLGSTSYLLPTETWDAWKRQLFEYDGVRYISSFAPLFVHQYSQAWFDFRNKHDKYADY
                     FQNSVTATRVHRQFCIDLASQFPDYSENLWGITASDSPRGYVVWGGPPATGPIDGTVV
                     PCASGGSLPFLPQECLQVLQTIKNKYGKRAWSRYGFVDAFNPLTDWYDTDVIGIDVGI
                     TMLMAENARTGFVWETFMKNPEAQRGMKRAGFVPNSPTPLSKS"
     gene            complement(3745..5301)
                     /locus_tag="VN788_09060"
     CDS             complement(3745..5301)
                     /locus_tag="VN788_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 39"
                     /protein_id="HXU20715.1"
                     /translation="MNLSRNFLVRAFVAWLCLALLCLPAQTLAQPASGQESSAQTETQ
                     TETIEVDPSAPSHPFPHYWENMFGSGRAILSLRDSYRRDLREVKQATGFEYIRFHAIL
                     DDDVGLYDEDAAGHPIYNFSYIDQIYDGLLENGVRPFVELSFMPKKLAEKQAVQAFWY
                     APVVSPPKDWDRWGDLISQLARHLIARYGIDEVSQWYFEVWNEPNLDFWAGDPKEATY
                     YHLYDVTAQALKKVNSRLRVGGPSTAQAAWVDRFLRHCAANHIPVDFVSSHVYANDSA
                     HDVFGTNESIPRTQMVCRAIHKVHDQVAASSLPALPIIWSEYNASYKNEPDVTDSIFM
                     GPWLADTIRQCDGLVDMLSYWSFSDVFEEQGVVKKPFYGGYGLIAEDGLPKPSFSAFK
                     LLHRLGDRRLAETSDSALVTRRADGRLVIAIWNESLPEQAGESKTVILHVKGFNGRHR
                     AAIYRVDSTHGSLMDAYTSMGKPVNPTQNQIAQLRRAAEIPPPESQALKHAEIKLTLP
                     PKGLALIELQ"
     gene            complement(5353..7185)
                     /locus_tag="VN788_09065"
     CDS             complement(5353..7185)
                     /locus_tag="VN788_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU20716.1"
                     /translation="MSNTSPHELPAPAPKWRERLAALRRVPPIFKMVWTCAPGVVLAS
                     VVTRLFVSVVPVALLAVTRFIIDALVNLGHHTALPGTFWWLVVLEFVLATSTTSLGRV
                     IDFCDTVLADKFTKNISTLTMEHASRLDLIRYEDPLFYDKMERARVQGTDRVVLIQMT
                     GRLVQEFITTISFASAIVFFSPWLFVFLIVCVVPAFLGETHFAFLGYSLNFEQTPAKR
                     QMEYLRILGGSRESAKELKLFGLGPFLVQRYKGLSDKLHLQNVGLAKRKLLFGTLLTC
                     LGTLGYYGAYGYVIYLATTGSLTIGKLTMLAGAIAGASTNIQSLFTTFSGIADQSLFL
                     TDLVEFFAVKPKILSKPGALLAPRPICQGIEFRNVSFSYPGNRRRVLSNINMRLDPGE
                     RIALVGANGQGKTTVVKLLTRLYDPTEGQILLDGIDLREYDLDDLWREIGVIFQDFMH
                     YEMTASENIAVGRIEEANNPFRIRAAAMKSFAENVIHKLPKGYDQLLGCRFEGGVELS
                     GGEWQKIALARAHLRDAQLLILDEPTAALDAHSEHEVFKRFADLTSGKMSLLISHRMS
                     TVRMADRILVMENGGIAEQGPHEQLVKHGGLYAEMFELQAASYR"
BASE COUNT         1907 a         2151 c         2407 g         1702 t
ORIGIN      
        1 ggtagaaaac ctcggcttct ccgttgagat gatccagccg ccgctccacc acttctcctg
       61 tcgcgtcatc cagcatggcc atctgctgat agcgcgcgtg aaaatcgcag cctataatca
      121 acattgctcg gctccttcct ccgagctttg gtcagttcag cgactaccaa gtctactcgg
      181 tcgcaaggag ccgacgccgt tatccaatca accaattcaa aagcagagcg caaggcggtg
      241 atcgggagat cggcgttccc agggcgtttg ggcggagtac gaggatacga ggaaaatctt
      301 tacatcgggg gtcgaatcgg cgcaagtagt tgttggtgta aagaaaaatc gtccacggtg
      361 aaaggcgcgc gagatgcctt agcccgaggc tgccgggcta aaagcccggc attacgtcca
      421 aaccttaacg caaaggcgcc cgcctaaaaa tcgaggcggc cgctacaacg acagtggcaa
      481 aggcaacggc cgggcgcagc gagcagcgcc acgacgcaag caaaagtctc ccccgccctt
      541 ccctgaaaat gccgccgtaa ttctttcctg cgtcagggca agcgggataa cgatgaggac
      601 acggccctcc cgttaggccg acgcttttct tttgccgccg cacgcattac gatggagcga
      661 ccattcaacc aggcagttcg aatgtcattt cctcataaga acatccgtct tcaccgattc
      721 agctatagcg gccaatcttc gtatttcgtt acgttgtgct gcgcgggcaa gcgtcccgta
      781 ttcgcaaaca cgaaaaatgc agtgtggttg gtcgagattc tgcgcaagca ctcgaatgcg
      841 caccgatttg cgatttatgc ctactgcgtg atgcccgacc atttccacgt tcttgtcttc
      901 ggcctcgatc cgacgagcag cctgctggct tttgtcaaga acctaaaact gacaacgtcg
      961 cgcggatata tgaaggattt cggtagagta ctctggcaaa agaaattcta cgaccacatc
     1021 ctcaggccgc gagacaaccc cgcagccgtc gccgcataca tctggatgaa cccagttcgc
     1081 aaggaactct gcaaagaccc acggcagtat ccttattcgg gttcattcgt tatggattgg
     1141 aagaaggcta tttcgcacgt ggaatcgtgg gtgccggatt ggaaggaaaa ggcgcccgcc
     1201 taaaaatcga gggggccgct acaagggcgg agatggccag tcatgcttgc acttgaccgg
     1261 gtgtgcgggc ggatgataac aatcggcagc ggaataatag aagcgagcag ggagaaatgg
     1321 cgtgagcact ttatcagcac gaagagcctt gcgagttgcg ggcatcggag cggttgccgt
     1381 ccttgcattg acttggatcc cacgcgcaaa cgggcaattg ccgggcggcc agatgggggc
     1441 cagcgttcct aaccagactc aaatcggcca atcgggtggc acgatgaacg acgacttgca
     1501 gtacggaaca ttcgggcatg gggtcgaacg agaacaagcc aacgcctacc aggtatttct
     1561 cagggaacga gacccagcca gaaagattcg gctcggaaac gattttctga agaagtatcc
     1621 gaagagcgcg ctggcagagc gggtggacgc cgagctgatg aacgtttacc gcgcgaagca
     1681 ggattggaag aatgtttatt tgtgcgcgga tcatgcactg gcgctgcagc ccgatgatgt
     1741 ggacgtgctt accaccgtgg gttggacgat cccgcatgtt tacaatccaa gtgatccgga
     1801 cgccgatcag gaattgaaaa ccgcggagac atacgcgaag catgccatcg aagttctggc
     1861 caaaatgccc aaaccggcgg gcttgacgga cgagcagttt gcggcggcca aagcggcaag
     1921 gacatttcaa gcgcacagcg cgctgggcct cgtctatttc cggcgccagg atttcagtga
     1981 ttcggcgagg gagcttgcag aatccacaaa gggcaacccg agacaggatc cgaccgatct
     2041 ctacgttttg ggagttgacc tgcaaaactt gaatcgtcac agcgaagcgg cggcggcttt
     2101 tcgcggatgc agccagctcg agggagttct gcagaatcaa tgcaagcaga gcgcgcaagt
     2161 ggcgcaagcg caggcggata aatcgaaggc gaggtaaatc ccaccgaccg ttctatcagg
     2221 cacatcgcgg caaagagagc acggtattgc cccaagccac cgggggtgcg gggatgggtt
     2281 cgcggttgac gatgagtgct tcatcggctt cccgggaatg aatataatca ttgcgcgaaa
     2341 tgaattcggg ataccgtttt ccaagaaggc tgactcgcag gaggcttctg aaggagggag
     2401 cgaaggcggg attgtgcgcg ccgttgctat tgtcgtctcc gctgcagctg gccggcttgc
     2461 tgcaagggaa tcaagctccg caagctgggc aaggagcggg aacgagcccg ttctatcaaa
     2521 tagacgaggc gtttctcgaa gcggtggaga acgcgcactt tcagtttttc tgggaacagg
     2581 cgaatccgga tacgggcatc gttcgcgacc ggtgcaacgc gatcgcgccg gacaagagcg
     2641 atttgggaag cattgcctcc ctcggctttg gactgacggc gctatgcatc ggcgacaagc
     2701 gcggttacct gccgcataac gaagcgcgcg gccgcgccct gaactcactg cgctttttgt
     2761 ggaagaagct ccccaatcac cgcggatttt tttatcactg ggcaaacgtt aacaccggag
     2821 aaaggctttg ggattcggaa gtttcgtcga ttgacaccgc gattttactg tgcggcgtgc
     2881 tgacgtgcgg cgcgtatttc gaacattctg aaatttcgga gctagcttcg atgatcttta
     2941 accgcgtcga ttggagctgg ctctcggaag atacgacgct gctgccgcat ggctggaggc
     3001 ccgaaacggg attcctgcag tatcgctggg acgattatag cgaaatgatg atgatgtacc
     3061 tgctcggatt agggtcgact tcgtatctgc tgccgaccga aacctgggac gcgtggaagc
     3121 ggcaattgtt cgagtacgat ggcgtgcgat acatcagctc gttcgcgccg cttttcgttc
     3181 accaatattc gcaggcgtgg tttgatttta ggaacaagca tgacaagtac gcggattatt
     3241 ttcaaaattc ggtgacggct acgagagtgc accgtcaatt ctgcatcgat ttggcgtcac
     3301 agtttcccga ttacagcgaa aacctttggg gtatcaccgc ttcggattcg ccaagaggct
     3361 acgtggtatg gggaggaccg ccggcgacgg gccccatcga tggaacggta gtcccgtgcg
     3421 cttcgggagg ttccctgcca ttcttgccac aagagtgcct gcaagtgctg cagacgatta
     3481 aaaacaagta cggaaagcgc gcttggtcgc gctacggatt tgtggacgcc tttaatcccc
     3541 tgacggactg gtacgacacg gacgtgatcg gaatcgacgt aggaatcacc atgctgatgg
     3601 ctgaaaatgc gcgcaccggt tttgtttggg aaacgtttat gaagaacccg gaagcacaac
     3661 gcggcatgaa acgagcgggc ttcgtgccga acagtcctac accgctctcg aaatcctagc
     3721 ggttgggctg caatccatcc ttcgctattg gagttcgatc agagcaagtc cctttggcgg
     3781 aagagttaat tttatttcgg cgtgcttcaa ggcttggctt tcgggcggcg ggatttcggc
     3841 agcgcgccgc agctgggcga tctgattctg cgtcggatta actggctttc ccattgaggt
     3901 gtaggcatcc atcagggagc catgcgtcga atccacgcga tagatggcgg cgcggtggcg
     3961 gccgttgaaa ccctttacat gaaggatcac ggttttagat tcgcctgcct gctcaggcaa
     4021 ggattcgttc caaatcgcga ttacaagacg gccatccgct cggcgagtga cgagggcgga
     4081 atcggatgtt tcggcaagcc gccgatcacc caagcgatgg agtagtttga aggcgctgaa
     4141 ggagggctta ggaaggccat cctcggctat tagcccgtat cctccgtaaa atggcttctt
     4201 cacgacacct tgctcctcga acacatccga aaaactccaa taagaaagca tatctaccaa
     4261 cccgtcgcac tgccgaatcg tgtctgctaa ccacggcccc ataaaaatgg aatcggtaac
     4321 gtccggctcg tttttgtagc ttgcgttgta ttcgctccaa atgatgggca aagccggcaa
     4381 cgaggaagcc gccacctgat cgtggacctt gtgaatggcc cggcaaacca tttgcgtcct
     4441 cgggatggat tcgtttgtgc caaagacgtc gtgagccgaa tcgttggcat agacgtggct
     4501 ggaaacaaaa tcaaccggga tatgattggc cgcgcagtgg cggaggaaac ggtcgaccca
     4561 ggcggcttga gccgtggaag gtccgcctac gcgcaagcgc gaattcactt ttttcagagc
     4621 ctgggccgtg acatcgtaaa ggtgatagta ggttgcttcc tttggatcac cggcccagaa
     4681 atcaaggttg ggctcattcc atacttcgaa ataccactga gagacttcgt cgattccata
     4741 acgtgcgatc agatggcgag ctagttggga aatcaaatcg ccccaacgat cccagtcctt
     4801 gggcggagat acgactgggg cataccaaaa agcttgcacc gcctgctttt cggctagttt
     4861 cttgggcatg aaacttagct caacgaacgg gcggactcca ttttcgagca aaccatcgta
     4921 gatctggtca atgtaggaga agttatagat tggatggcct gcggcgtctt catcgtagag
     4981 gccaacatca tcgtccagga tcgcgtggaa acggatgtat tcgaaaccag tggcctgctt
     5041 cacttcgcgc agatcgcggc gatagctgtc gcgcaagctc agaatggctc ggccagaccc
     5101 gaacatattc tcccaataat gaggaaaggg atgactggga gcagagggat cgacttcgat
     5161 ggtttcggtt tgagtttctg tttgggctga tgactcctgt cctgatgcgg gctgggctag
     5221 ggtttgagcc ggcaggcata acagggcgag gcagagccac gcgacgaacg cgcggaccaa
     5281 aaagtttcgg ctgagattca cggaaggctc cgcggcgcta ggaagttgcg atttcgggaa
     5341 tttgcgcgcg aattagcggt aacttgccgc ttgcagctcg aacatttcgg cgtagaggcc
     5401 gccatgtttg acaagctgct cgtgcggtcc ttgttcggca attccgccat tctccatcac
     5461 gaggatgcgg tccgccatgc ggaccgtgga catccggtgc gaaatcaaca aggacatttt
     5521 gccagaagtg aggtcggcaa agcgcttaaa gacttcgtgc tctgagtggg cgtcaagggc
     5581 cgcggtcggc tcgtctaaaa tcagcaattg ggcatcgcgc aggtgggcgc gggccaaagc
     5641 gatcttctgc cactcgcctc cggacagctc gacgccgccc tcgaaccggc agccgagaag
     5701 ctgatcatag cctttgggga gtttatggat gacgttctcc gcaaaactct tcatcgcggc
     5761 cgcgcgaatc cggaacgggt tattggcctc ttcaatgcgg ccgacggcga tgttttcgga
     5821 tgcggtcatc tcatagtgca tgaagtcctg aaagattacg ccgatttcgc gccagaggtc
     5881 gtcaagatcg tattcacgaa gatcgatgcc gtccaaaaga atctggccct cggtcggatc
     5941 gtataaccgc gtgagcagct tgactacggt ggttttgccc tgcccattgg cgccgacgag
     6001 ggcgattcgc tctccaggat cgagccgcat attgatgttg gaaagtacgc ggcgccggtt
     6061 tccaggatag gagaaggata cgttccggaa ttcaattccc tggcatatcg ggcgcggagc
     6121 cagcagagca cccggcttcg agagaatctt cggcttcacg gcgaagaact ccacgaggtc
     6181 ggtgaggaag agcgattgat cggcgatgcc ggaaaatgta gtaaagaggg actgaatgtt
     6241 cgtgctagca ccggcaatag caccggcaag catcgttaat ttgccgatgg tcagcgatcc
     6301 ggtggtggcc agataaatga cgtaaccgta cgcgccgtag taaccgagag ttccgaggca
     6361 agttagcagc gtgccgaaca acagcttgcg cttggcgagg ccgacgttct gaagatggag
     6421 cttgtcagaa aggcccttgt agcgctggac aagaaacggt ccgagaccaa aaagcttcag
     6481 ctccttggca ctctcacggc tgcccccgag aatgcgcaga tattccatct ggcgcttcgc
     6541 cggggtttgc tcaaagttta gggagtaacc caaaaaggcg aaatgagttt cgccgagaaa
     6601 agccggcaca acacaaacga tcaggaaaac aaatagccag ggagaaaaga acacgatggc
     6661 cgacgcaaag ctgattgtcg tgatgaattc ttgcaccagc cgacccgtca tctggatcag
     6721 cactacgcga tcggtccctt ggacgcgcgc gcgctccatc ttgtcgtaga aaagaggatc
     6781 ttcgtagcgg atcagatcca agcgcgaggc gtgctccatg gtgagagtgc tgatgttctt
     6841 ggtgaacttg tctgcgagga cggtatcgca aaaatcgatt acccggccga gactagtggt
     6901 cgaagtcgcg aggacgaatt caagaactac cagccaccag aaggtgcccg gtaacgcagt
     6961 gtggtgccca aggttaacaa gggcgtcgat gatgaatctg gtgacggcca ggagggcaac
     7021 ggggaccacc gaaacaaaca gccgggtaac cacgctggcc agcactactc ccggcgcaca
     7081 ggtccaaacc atcttaaaaa ttgggggaac cctgcgaaga gccgcgaggc gctctcgcca
     7141 ttttggcgct ggggccggaa gctcgtgggg actggtgtta ctcatggact gggcggctgc
     7201 tctcatctca ccaacctgtc gaatgcgctc acgacgccca tggaaagcac gtgggttgcc
     7261 atgcaaaaag ctccgaagta gtagtaatgg gacgggttac gcagagctat atcccgggag
     7321 cacggcgatt tttgcgcgat tccgcaattt ctatctagta atggagcttg ccgttttggc
     7381 cggagggact cgagttcggc atatgtgcgg gagcgccagt tgcgaaaggg cggcgagagc
     7441 gagccgacct cccaccatgc gagggttaag ggccttcgct tccatcacca gacgtgctga
     7501 tttggtgaat cagggcctcc gctgagggcg gcactgcaat gtcaccgccg gtcggattct
     7561 gtaaagtagt gtcgccatac atggcctgat cgccgctgtt atctgcccgg acaacggcac
     7621 cgttgaggct aactccggca aataggcctg acagcgagaa ttactaaggc ctccgctttg
     7681 agcttcgaat cagtgtcagc agcagcgtga cgaccgacgg ggccggcagc aacgcttggc
     7741 gtctgcacct agcttgaatt tgtcactcag aagactctgc aacgcgcgat catttatgaa
     7801 cagcatagtg atatctgtcg acgaaccttc gatttttccg atttgagatc caacgctgcc
     7861 gccgcgggga gctgcgaaca tgggccgcgc tccatcgttg gcccacgtcg caatgccaag
     7921 gtcataattg ccacctagga tcaaggcgaa ccttacttcg cccggaaaat cctccagatg
     7981 agagcggaag cgacgcggga cggtggcgca gatctgcgaa tctcagacct gggttatccc
     8041 gctcatacat atcaatattt caccctatgg cgccaacatc cgcctaaacg tactctcctc
     8101 aggtactgaa gagaaggcgt gccacttgag gaattcgtct ttgaatgcgc acgattttct
     8161 ttcgaaa
//
LOCUS       DATNDT010000280         2205 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_244162, whole genome shotgun sequence.
ACCESSION   DATNDT010000280 DATNDT010000000
VERSION     DATNDT010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2205
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244162"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VN788_09070"
     CDS             <1..215
                     /locus_tag="VN788_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004093489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HXU20717.1"
                     /translation="AGWIAGQITRGQGFGCIVDIILGLIGAVIGGWLFTRLQIPAWGF
                     WGSLAAATVGAVLLVAIARLFAGGRS"
     gene            285..638
                     /locus_tag="VN788_09075"
     CDS             285..638
                     /locus_tag="VN788_09075"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU20718.1"
                     /translation="MSQSHADRRFQRIATPQGIWVSWGVRTAKVVSRVRDLNEGGMFV
                     STPNPEPVGTVLAILLVAREGEIRGHGTVRNTVPHEGMGVEITAMGAEDTARFRELLK
                     RLLARQPSAIPQDVE"
     gene            complement(987..1424)
                     /gene="mscL"
                     /locus_tag="VN788_09080"
     CDS             complement(987..1424)
                     /gene="mscL"
                     /locus_tag="VN788_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HXU20719.1"
                     /translation="MLKDFKEFAMRGSVLDMAVGIIIGAAFGRIISSLVSDVIMPPIG
                     LLLGKVDFSSLFVNISGKSYPTLAAAKAAGAATINYGLFVNTVLDFIIVAFVIFLFIR
                     QINRWSKPAQAPAPTTKECPYCYSAIPIKATRCPHCTSELKAA"
     gene            complement(1531..1890)
                     /locus_tag="VN788_09085"
     CDS             complement(1531..1890)
                     /locus_tag="VN788_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20720.1"
                     /translation="MPNVANLFRLLSEFIVLLLGALLIVVAISRPVGVPASPLALVIL
                     GIALIYWGARVWFRPGAEGNRLHAWVRGGSLLLVGICVVAIRWLPLRDESLLLGCAGA
                     ALVLRGLVGGIVFAWQK"
     gene            2068..>2205
                     /locus_tag="VN788_09090"
     CDS             2068..>2205
                     /locus_tag="VN788_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014863922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HXU20721.1"
                     /translation="MHKVIIIGSGCAGLTAAVYAARADLRPLVLDGYEPGGQLALTTE
                     VE"
BASE COUNT          447 a          702 c          610 g          446 t
ORIGIN      
        1 tggcgggctg gatcgccggt caaatcactc gcggacaggg tttcggctgc attgtcgata
       61 tcattttggg tttgattggc gctgtgatcg gcggatggct tttcaccagg ctccaaatcc
      121 ccgcctgggg tttctggggc agtctcgcgg cagccaccgt gggagcggtg ctgctcgttg
      181 cgatcgcgcg gctgtttgcg ggcggccgct cctaacccac ccgtggattt gcacaactac
      241 ttgcgacgat ttccaaattc cgtgccgctt tctatgaggg cagcttgagc caatctcacg
      301 cggatcgccg ctttcagcgc attgcgacgc cccaggggat ttgggtgagc tggggagttc
      361 gcaccgccaa ggtggtctct cgcgttcgcg acctaaacga aggtggaatg tttgtttcga
      421 cgcccaatcc agagcctgtc ggtaccgtcc tggcaattct gctcgttgct cgcgaaggag
      481 agatccgagg ccacggaacc gttcgaaata cggttccaca cgaggggatg ggcgttgaga
      541 tcactgccat gggcgcggaa gacaccgcgc gctttcgtga actcttgaag cgactcctcg
      601 ccagacagcc ctcggccatc ccgcaggatg tggaatgaag ccttcttccc tcaaatggtt
      661 cgcgtgttcc tggttcgcgc gcctacgccg ctttcaactc tgacgtgcag tgcggacatc
      721 gcgtagcctt gatccgaatg catttgtagg ggcacagcat gctgtgcccg gcgaacatgc
      781 ttggctacct ctcgccatcc gcctgggagc ttcggtggcg ccgtcatcct gcttgctcgc
      841 actgagcgca gcgaatgtgc cctaacgaag gaagggagcg acgtgtcccg cgctttttct
      901 cttgcgcgaa acgcccggac acacagtcgg agatctcttc agaacgcctc gcgccttgtt
      961 ctatccgccc aatccgtttg ccccgcctac gccgctttca actctgacgt gcagtgcggg
     1021 caccgcgtgg ccttgatcgg aatcgccgaa tagcaatacg gacattcctt cgtagtcggc
     1081 gcaggtgcct gcgcaggctt gctccaccgg ttgatctgcc gaataaaaag aaaaatcacg
     1141 aacgccacga taataaaatc gagcaccgta ttcacgaaca ggccatagtt gatcgtggct
     1201 gctcccgccg ccttggccgc cgctagagtc ggatacgatt tccccgaaat gttcacaaac
     1261 aagctcgaaa aatccacttt tcccagcagc agtccaatcg gaggcataat cacgtcgctg
     1321 accagcgagc tgatgatccg accgaaagcc gcgccaataa taattcccac cgccatgtcc
     1381 agcacgctcc cgcgcatggc aaattctttg aagtctttca gcatgcctgc ctcctcgcag
     1441 aatagttcag cgcgtttgtc gcgccgccca acttccgtgg ccgttgacct taacgagaac
     1501 cagaatgagt ggcaagaaga aacgcgttgc tcacttctgc cacgcgaaaa cgatgccgcc
     1561 cacgagcccc ctcagcacca gcgcggctcc ggcgcacccc agcaacaggc tttcatcgcg
     1621 caagggcagc caccgaattg ccaccacgca gataccgacc agcagcagtg atcctccgcg
     1681 aacccacgcg tgcaatcggt tcccttccgc tccagggcga aaccacaccc gcgctcccca
     1741 gtagatcaga gcgattccca ggatcaccag cgccagtggc gatgccggca ctcccactgg
     1801 acgggaaatc gccaccacaa tcagcagcgc tcccagcagc agcacgataa actcgctcaa
     1861 cagacgaaac agattcgcta cattcggcat cgcgtcgccc aagccgcgcg attatcccgc
     1921 ttgcgccgtg ggtaagcaag cagccgctaa tccggcggat cgtaccttcc aaggttttca
     1981 agtccgctgc gggattgcta tactgatagt gccattcggc tgcatcgccg cgctgcatgc
     2041 gggaatccaa tcgtacagga gaagtcagtg cacaaggtca tcatcatcgg tagtgggtgt
     2101 gcagggttaa ccgcggcagt gtatgccgcg cgcgcggatt tgcggcctct ggtcctcgat
     2161 ggctacgagc cgggcggcca gcttgctctg accaccgaag tcgag
//
LOCUS       DATNDT010000281         2803 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1023512, whole genome shotgun sequence.
ACCESSION   DATNDT010000281 DATNDT010000000
VERSION     DATNDT010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023512"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2260
                     /locus_tag="VN788_09095"
     CDS             <1..2260
                     /locus_tag="VN788_09095"
                     /inference="COORDINATES: protein motif:HMM:NF020289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXU20722.1"
                     /translation="DWAKGWLGWRRGNQSYARREHSTQRHTQEPAEEKPQQDKPGEQG
                     EAESSEELENRQSAGRNAENEIRRTKVDDILASCQPTPGTPVEQYLRTTRGIGATPPD
                     CIRYRPSAFRSYGALVALATDVEGEVLAVQQIYLTEQGTKAPVDVVKRTNKAVDMWAE
                     KAAVRFPGTGPLILAEGVETAMSIWQATGREAWACLGIANIGRAPVPDAAPVVIARDG
                     DEPGSKADRALVQAVNALRARGHSVCVAVPPTGEDFNDVLAKQGPDGVKRLVDDAKVS
                     TDSEPVMLEIGSDVEVAGRFYAVLVKKFGQVVHSEGKFWRYCETHWEPVPENELRCHV
                     HAFDGALYPVGKGFAPVRLGKGRIDSIINEAAALCASPDFFDLPPTGINCVAGFIRFS
                     EDGTPTLEPHKPDHRCRHTLPGRWSPDTPSDPPAGSLLFKLLNGVFQGDEDANEKIML
                     LAELCGSAALGYATRLKQPRAAIFHGVTAENGKSQILDAARGLLPKTAVCSVPAARMG
                     DERHIIGLVGKLLNATDELSASAVASDKFKSVVTGEPIDGRDVYRTRVEFRSLAQNLF
                     ATNTLPPFHGGMDRGVQRRLLVLPFNRSIPLGERIEGIGRRVAEEEPDLLLAWAVKGA
                     SQLLKHGEFTIPQSCKKALHDWIYDADPVLAWAQSCVEVEPIVNGWPSIKTRHAYDNF
                     KKWAVAEGYDERKLPYVNTFTTRVCGNLKGVEHHRKSTARVFLGLVIRREPPQGGSEG
                     RSEPESAEEIPF"
     gene            complement(2452..>2803)
                     /locus_tag="VN788_09100"
     CDS             complement(2452..>2803)
                     /locus_tag="VN788_09100"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HXU20723.1"
                     /translation="FILYGRAHGALTMHSDALVVACQVAGFSPRVGHVVSNNLSRLNF
                     VAAGLGIAVVAASFQRINIDGVVYRRLGGPLQPRAPLNLASRRGDTSAVVQQFLKLAK
                     QTARNFQADLEKAS"
BASE COUNT          646 a          756 c          830 g          571 t
ORIGIN      
        1 tgattgggcg aaaggctggc tgggctggcg acgcgggaat caatcctacg cgcgcagaga
       61 gcattcgacg cagcgtcaca ctcaagagcc cgctgaagag aaaccacagc aggataagcc
      121 gggcgagcag ggagaagcgg aatcttctga ggaactcgaa aaccgtcagt cggctgggcg
      181 aaacgcggaa aatgagattc ggaggacaaa ggtcgatgac atcctcgcca gctgccaacc
      241 gaccccgggt acgccggtcg agcaatacct ccgaacaact cgtgggattg gcgcaacgcc
      301 gccggattgc atccggtatc ggccaagcgc atttagatct tacggtgcat tggtggcact
      361 cgcgacagat gtggaaggcg aggtcctggc tgttcaacag atctatctga ctgaacaggg
      421 cacaaaggcc ccagtcgacg tcgtcaagcg caccaataaa gcggttgata tgtgggcaga
      481 gaaggcggcc gttcgcttcc ccggcaccgg gccgctgatt ttggccgaag gtgtcgagac
      541 cgcaatgtcg atctggcaag caaccggacg ggaagcctgg gcatgtcttg gtattgcaaa
      601 cataggcagg gccccggtgc cggatgcagc cccagtcgtc attgcacgtg acggcgacga
      661 gcctgggtcc aaggccgacc gggccttggt ccaggccgtg aatgcgctgc gtgcccgagg
      721 ccactcggtt tgcgttgctg tcccgccgac aggggaggat tttaatgacg tcttggcgaa
      781 acaggggcct gacggcgtta agcggctcgt cgacgacgcg aaagtatcaa ccgactcaga
      841 acctgtcatg ctcgagatcg gatccgacgt ggaggttgcc ggtaggtttt atgcagtttt
      901 ggtcaaaaaa ttcgggcagg tggtgcactc cgagggtaag ttttggcggt actgtgaaac
      961 ccattgggag ccagttcccg agaacgaatt gcgttgccat gtacatgcgt ttgatggagc
     1021 gctttacccg gtaggaaaag gttttgcccc ggtaagactc gggaaggggc gcatcgattc
     1081 cattatcaac gaggcggccg ccctttgtgc ctctccggat tttttcgatt tgccaccaac
     1141 cggaatcaat tgcgtggcag gattcattcg cttttccgag gacggaactc ctacccttga
     1201 gccgcacaag ccggatcatc gctgccgaca caccttgcca ggacgttgga gcccggacac
     1261 acctagtgat ccgccggcgg ggtcgctact gttcaagctt ctcaatggag tgtttcaggg
     1321 cgacgaagat gcgaacgaga aaatcatgtt gctcgccgaa ttgtgtggtt cggctgcgct
     1381 aggctacgcg acgcgactga agcaaccgcg ggcagcgatc ttccacggtg taaccgctga
     1441 gaacggcaaa agccaaattc ttgacgccgc gcggggtcta ctccccaaaa ccgcagtctg
     1501 ttccgttccg gctgctcgaa tgggcgatga gcgccacatc atcggtcttg ttggaaagct
     1561 gctcaacgcc acggacgagc tttcggcgag cgcggttgcc tcggacaagt tcaaatccgt
     1621 agttactgga gagccgattg acggtcgcga cgtttatcgc acccgagtag agttcagatc
     1681 gctcgctcaa aacctattcg cgacgaatac gctacctccc ttccatgggg gcatggatcg
     1741 tggcgtccag cggcgacttt tagtgctgcc attcaaccgc tcaattccgt tgggcgaacg
     1801 aattgaaggc atcgggcgcc gcgttgcaga ggaggaacca gacctgctct tagcatgggc
     1861 ggttaaagga gcctcccagc ttctcaagca cggtgagttc acgattccgc agtcttgcaa
     1921 aaaggctctt catgattgga tttacgatgc tgatcctgtg cttgcctggg cgcaatcatg
     1981 tgtcgaggta gaaccgatcg taaatggttg gccgagcatc aaaacccgac atgcttatga
     2041 caatttcaag aagtgggccg tcgctgaagg ctatgatgaa agaaagcttc cttatgttaa
     2101 cacattcacg acacgggttt gcggcaacct aaagggcgtc gaacaccatc gtaagagcac
     2161 tgcccgagtt ttcctcggac tggtcatcag gcgagagccg ccgcaaggcg gatccgaagg
     2221 tcgatccgag cccgagtcgg ctgaagagat ccctttttga ttgcttgcta tctaagagcc
     2281 gtcgtcgatt agaataattc ttgagattgc atgaccggac tacgtctagg gccatgtact
     2341 cataaaaccc gcagcgatgt ccgcttcgat ctaaaagcga ccgaatttcg cactgcagcg
     2401 aaatgacgcg acgtgccaac agcggtcatt aagtcaaaga tggtagccgc atcacgatgc
     2461 tttctccaag tccgcctgaa aattcctcgc ggtttgcttg gccaatttca ggaattgttg
     2521 aactacagcg gaagtgtcac cgcgacgcga tgcaaggttt agcggagccc ttggttgcag
     2581 ggggcctcca aggcgacgat agacaacgcc gtcaatattg attcgctgga acgaggctgc
     2641 aacgacggca atgccgagcc cagcggcgac gaagttcagc cttgataaat tatttgagac
     2701 gacgtggccg acacgagggc tgaagcccgc cacctgacat gccacaacca gcgcatcgct
     2761 gtgcatagtc aaagcgccat gcgcacgccc gtagagaatg aat
//
LOCUS       DATNDT010000282         3169 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2811052, whole genome shotgun sequence.
ACCESSION   DATNDT010000282 DATNDT010000000
VERSION     DATNDT010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3169
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2811052"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VN788_09105"
     CDS             complement(<1..726)
                     /locus_tag="VN788_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-bladed beta-propeller"
                     /protein_id="HXU20724.1"
                     /translation="MKWCALWLFIFAAAAATFSQQASESAKVPEISFRSVPDFLKLPP
                     DLYLGEASGVAVNSKGHVFVFSRGNTTGPAYGASAAQLLEFGADGHFIREIGHNLYAW
                     SFAHTVKIDPQDNIWATDKGSDMVIKFNPAGRVVMVFGRKQEASDEGTGPLKHVRPPL
                     PPVDGLFRQVTDVAWDAQGNTYISDGYINSRVAKVDKNGNWLKSWGTPGDGPGQFNTP
                     HSIAVDAKGNVYVADRGNRRIQVF"
     gene            833..2275
                     /gene="proS"
                     /locus_tag="VN788_09110"
     CDS             833..2275
                     /gene="proS"
                     /locus_tag="VN788_09110"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HXU20725.1"
                     /translation="MSDLKLTTRAEDYSEWYNQVVLRAELADYAPVRGCMIVRPYGWA
                     LWENITAQLDRRFKATGHVNAAFPLLIPRSFIEKEKSHVAGFSPELAVVTHGGGEELE
                     EPLVIRPTSETIIGHAYAKWIQSYRDLPVLINQWNSVVRWELRTRLFLRTLEFYWQEG
                     HTAHATAEEAEAETRQMLDVYTDFAVNEAAIPVIPGRKSAAERFAGAEQTYSIEAMMG
                     DGKALQSGTSHNLGQNFAKAFEIRYLDKGGVLQHCWTTSWGLSTRFVGAIIMVHGDDQ
                     GLILPPRLAPHQIVIVPICKTDEERGSVMENAQRLKRELVDAGIRVKMDEREGMSPGF
                     KFNDWEMRGVPLRLELGPKDVAKGSVVLARRDRPGREGKAFVPQQGLAAAVAGTLEEI
                     QKALYDRALEFRKANTAEPTDYGEFKKAVEKGFAFSWWCGSSECEEKIKEETKATMRC
                     MPLDQAAGGGGKCVYCGKAAGEKAIFARAY"
     gene            2544..2927
                     /locus_tag="VN788_09115"
     CDS             2544..2927
                     /locus_tag="VN788_09115"
                     /inference="COORDINATES: protein motif:HMM:NF027463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4845 domain-containing protein"
                     /protein_id="HXU20726.1"
                     /translation="MLRLREITEERGGAKRNLIITLLILGSMIFAGIKVVPIYFDNYQ
                     FQDAIESESRFALTGYPKKSPDDIREDVWQKAQELSIPAQKEDIQVNETNGSVSISLD
                     YSVPVDLAVYQLTLQFHPHADNHSI"
BASE COUNT          685 a          792 c         1042 g          650 t
ORIGIN      
        1 gaatacctgg atgcgccgat taccacgatc ggcgacgtag acatttccct tcgcatcgac
       61 tgcgatgctg tgcggcgtgt tgaactgtcc ggggccatcg ccgggagttc cccaggattt
      121 cagccaattg ccgtttttat ccactttggc tacgcgggaa ttgatgtagc cgtcgcttat
      181 ataggtattt ccttgcgcat cccaagcgac gtcggtcacc tgccggaaaa gaccgtcaac
      241 tggcggaagc ggagggcgca catgcttcaa tggcccggtg ccttcatcgg aggcttcctg
      301 cttgcgtcca aaaaccatca ccacccgccc ggcgggatta aatttgatta ccatgtctga
      361 acctttgtcg gtggcccaga tgttgtcctg cggatcgatt tttacggtgt gggcgaagga
      421 ccaggcgtag aggttgtggc cgatttcgcg gatgaaatgg ccgtcagcgc caaattccaa
      481 cagctgggcg gcactggcgc cgtaggctgg tccagtggta ttgccgcgag aaaagacgaa
      541 tacgtgtccc ttggaattga cggctacgcc ggaggcttct ccgagataca aatccggcgg
      601 gagcttcaga aaatcgggaa ccgagcggaa ggagatctcc ggaactttcg cggactcaga
      661 cgcttgctgc gagaaagttg ccgcagcagc agcgaaaata aataaccaca gcgcacacca
      721 tttcatagct gcctccaatc gccgagagta tagccgatgc ggcgcggatt gacttcgggc
      781 ggtgagtctc ctaacctaac ccatccatcg cacggaaaag agatacgaga aaatgtccga
      841 tttgaagctg actacgcggg ccgaagatta ttcggagtgg tacaaccagg tggttctgcg
      901 cgcggagctg gcggattacg cgccggtgcg cggctgcatg atcgtgcggc cgtatgggtg
      961 ggcgctgtgg gaaaacatta cggcgcaact ggacaggaga ttcaaggcga ccgggcacgt
     1021 gaacgcggcg tttccgttgc tgattccacg cagttttatt gagaaggaaa aatcgcatgt
     1081 ggcgggattt tctccggagc tggcggtggt gacgcacgga ggcggcgagg aattggaaga
     1141 gccgctggtg attcgtccta catcggaaac gatcatcggg cacgcgtacg cgaagtggat
     1201 acagagctat cgcgatttgc cggtgctgat caatcaatgg aatagcgtgg ttcgctggga
     1261 actgcgaacg cgattatttt tgcgcacgct ggaattttat tggcaggaag ggcacacagc
     1321 gcacgcgacg gcggaggagg cggaagccga gacgcggcag atgctggacg tgtacacgga
     1381 tttcgcggtg aacgaggcgg cgatacccgt gattccgggg cgaaaatccg cggcggaacg
     1441 ttttgcgggc gcggagcaga cctattcgat cgaagcgatg atgggagacg ggaaggcttt
     1501 gcaatcgggc acgtcgcaca atttggggca gaatttcgcg aaggcgtttg agatacggta
     1561 tctcgacaag ggcggcgtgc tgcaacactg ctggactact tcctggggac tttcgacgcg
     1621 gtttgtcggc gcgatcatca tggttcacgg cgacgatcag ggattgattc tgccgccgcg
     1681 attggcgccg catcagattg tgatcgtgcc aatctgcaag acggacgagg agcgcggcag
     1741 cgtgatggaa aacgcgcagc ggctgaagcg ggaacttgtg gatgcgggca ttcgcgtgaa
     1801 gatggacgag cgcgaaggga tgagccccgg attcaagttc aacgactggg aaatgcgcgg
     1861 tgtgccactg aggctggagc ttggtcccaa ggatgttgcg aagggttcgg tggtgctggc
     1921 gcggcgggat cgccctggca gagagggtaa ggcgtttgtg ccgcagcagg gattggcagc
     1981 ggcggtggcg ggaacgctgg aggaaattca gaaggcgctg tatgaccgcg cgctggaatt
     2041 tcgcaaggcg aatacggcgg agccaaccga ttacggcgaa ttcaagaagg cggtggaaaa
     2101 aggcttcgcg ttttcctggt ggtgcggcag cagcgaatgc gaagagaaga ttaaggaaga
     2161 gaccaaggcg acgatgaggt gcatgccgct cgaccaggcc gccgggggcg gaggcaagtg
     2221 cgtttattgc gggaaggcgg ccggcgagaa ggctattttc gcccgggcgt attgagcgcg
     2281 ttggatcgtt acctttcgcg agcctgccct tcggcttccc tcagggtaaa aaggcccgcg
     2341 cttccaccgc gagctacggc ccattttcaa gtgccttcgc gcgtcaggcc cgcggtacca
     2401 ccccgtgaaa cgggcaactt tccgtgcgaa cccggcgttt accgatcgca aacacggata
     2461 ttcggcgatg ccccgcgatg cgtgtcctcc gtgatagcct tgggggaatt ctgcaaagaa
     2521 ctaggcggcg gaggatcagc cacatgttga gattgaggga gatcacagag gagcgcgggg
     2581 gggcgaagcg gaacctcatt attacgctgc tcatcctggg ctctatgatc ttcgcaggga
     2641 tcaaagtggt ccccatctat tttgataatt atcagtttca ggatgcgatc gagagcgagt
     2701 cgagatttgc gctgacgggc tacccgaaga agagccctga cgacatccgc gaagatgttt
     2761 ggcagaaggc gcaggaactt tcgattcccg cgcagaagga ggatatccag gtgaacgaga
     2821 ctaacggaag tgtttcgata agcctggact attcggtgcc ggtcgatttg gcggtttatc
     2881 aactcacgtt gcaatttcac ccacacgcgg acaaccattc gatctaaaat aagattttga
     2941 tcttttttgc ggattagaaa cgcgccgggc aacccagcca tcggcgcggg ctcgcctgat
     3001 cgcgccctgt ctctcggtac gcgaccggcc ggagaaacgt attgcggatt cgaattgccc
     3061 gggggttttg gacatcgcgc cttgggatcg gggtgcttgg ggcggcgctg gtcgcgctgc
     3121 ttgtggcgac gattgtcttc gcaggatatt acattcaatt cggacattt
//
LOCUS       DATNDT010000283         3563 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2658078, whole genome shotgun sequence.
ACCESSION   DATNDT010000283 DATNDT010000000
VERSION     DATNDT010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2658078"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..917)
                     /locus_tag="VN788_09120"
     CDS             complement(63..917)
                     /locus_tag="VN788_09120"
                     /inference="COORDINATES: protein motif:HMM:NF039362.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6159 family protein"
                     /protein_id="HXU20727.1"
                     /translation="MGKLSRSWDLVGKSFGILLSDKELLYLPIASGIACLAVSVMMLA
                     GYALAFPAQISAFAATSGAHQSPVTQGMWAYLFFFYLVNYFIVVFFNVALVAAASDRL
                     AGGNATINYGLQVAWRRKGKIFQWALLAATVGILLRALEQRLGWLGRLVTKFIGVAWT
                     MATFFVVPLLAAEDIGPAEALVRSGELFTETWGEQLIGGFSFGLIFVLLALPGIALPF
                     LLRSPLGSSGVLMGVILMIVYWLLLSVVSAAVQGIFMAALYRYATTKEVPPTFHLSDF
                     STAWQPKD"
     gene            1062..2099
                     /locus_tag="VN788_09125"
     CDS             1062..2099
                     /locus_tag="VN788_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXU20728.1"
                     /translation="MSATTATPLPSVNRQFVLAARPVGMPKESDFRLVEAPIPALTNG
                     QALIRTLFLSVDPYMRGRMTGVRTYADPVDIGQLMVGATVGKVLQSLNPQLQAGDIVA
                     GYWGWQEYAISDGRGLQKLDASRAPISTALGVLGMPGMTAYFGFLDLCQPKPGETVVV
                     SGAAGAVGSLVGQIAKIKGCRAVGVAGSDQKIAWLTGELGFDGAFNYKTTQDYAAKLK
                     ELCPKGIDCYFDNVGGNVTDAVLPVLNVRARISVCGQISQYNATKPELGVRPYIFLLS
                     KQCRAEGFIVTQFMERYPEGVAQMAQWIKEGKLKYREHIVNGFENAPRALIGVLQGEN
                     TGKMLVKFADA"
     gene            complement(2282..3553)
                     /locus_tag="VN788_09130"
     CDS             complement(2282..3553)
                     /locus_tag="VN788_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXU20729.1"
                     /translation="MTSTTVSAKGPFAGTPEFEKNLDRLAEVAIRAGLGLAPGQELVM
                     TATLDSVPLARLIIEHAYKAGASLVTTLFSDEQSALLRFRYAPDASFDVAPAWLYEGM
                     AQAYRNGAARLAITGNNPSLLSKEDPEKVSRLNRATSKAYRPALELITRHEINWTIIA
                     CATPEWAAAVFPDLPQDEALARLWTAIFAASRADQPDPLAAWKKHDAHLHARADMLNQ
                     KRYSALHFRGPGTDLRVGLADDHLWLGGGTTAGNGRYCIPNMPTEEVFTTPHKDRVDG
                     RVTSTKPLSHQGTMIEEISVRFQAGKIVEAHAARGEQVLQRMIETDEGARRLGEVSLV
                     PHSSPIAASGLLFMNTLFDENAACHIALGQAYSTCLKNGDTLTPEQLASKGANESLIH
                     VDWMIGSNRIDVDGISASGTSEPVMRAGEWV"
BASE COUNT          765 a          973 c         1138 g          687 t
ORIGIN      
        1 gatccccgga aagataaaga gtcggcacgc ttcgctcaga atgacgacat ttgcggcgcg
       61 ggctaatcct tgggttgcca ggctgtggag aagtcggaga ggtggaaggt gggcgggact
      121 tctttggtgg tggcgtagcg atagagggcg gccatgaaga tcccttgcac ggcggcgctg
      181 acgacagaaa gaagaagcca atagacgatc atcaaaataa cacccatcaa tacgccgctt
      241 gagcccaagg gtgagcgcaa taaaaatggg agcgcgatac cgggaagtgc gagcagcacg
      301 aaaatcaacc cgaaactgaa tccaccgatc agctgctcgc cccaggtttc agtgaacaac
      361 tcgccggaac gcacaagggc ttcggcgggg ccgatgtctt ccgccgcgag gagcggcacg
      421 acaaagaagg ttgccatcgt ccacgccacg ccgatgaact tcgttaccag acgccctagc
      481 caaccgaggc gctgctcgag cgctctcaaa agaatgccaa cggtcgcggc taggagcgcc
      541 cactgaaaga ttttgccttt gcgccgccag gcgacctgaa ggccataatt gatggttgca
      601 ttgccgccgg cgaggcgatc ggaagccgcg gcgactagcg ccacgttgaa gaaaacgacg
      661 atgaagtagt tcaccagata gaagaaaaac aaataggccc acatcccttg cgtgaccggc
      721 gactggtgcg cacccgatgt cgcagcaaaa gcggaaattt gcgcagggaa agccagagca
      781 tacccggcca gcatcattac ggaaacagcc aagcacgcaa tcccggaagc gatcggcagg
      841 tacaacaatt ccttgtccga tagcaaaatt ccgaaactct ttccgacgag atcccagctt
      901 cgagaaagct ttcccatacg tgctccccgg gcgcctgcgt tggagacaat actacctgaa
      961 atctctcact cggacttctg ggttgtttcc gcggaggcca ttcagccgag tagcgcaact
     1021 tgaacgcgat ttcggcggac tctggcataa taccggctgt catgtctgcc actactgcca
     1081 ctccgctgcc cagtgtgaat cgccaatttg ttctggccgc gcgtcccgta gggatgccga
     1141 aggaatctga ctttcggttg gtcgaagcgc cgattcccgc gctcacgaac ggacaagccc
     1201 tcatacgaac gctttttctt tccgttgatc cctacatgcg cgggcgaatg accggcgtgc
     1261 gcacatacgc cgaccctgtc gatatcgggc aactgatggt cggagcgacc gtcggcaaag
     1321 tgcttcaatc gctgaatccg caattgcaag cgggcgacat cgtggcgggg tattggggct
     1381 ggcaggagta tgcgatttcc gacggacgcg gtttgcagaa gctggatgcg agccgcgcgc
     1441 ccatctcaac agcgctgggc gtgctgggaa tgccgggaat gacggcctat ttcggttttc
     1501 tcgatttatg ccagccgaag ccgggcgaga cagtggttgt ctcgggagcg gcgggagccg
     1561 tgggctcgct tgtcgggcag atcgcgaaga tcaaaggttg ccgcgcggtt ggcgtggccg
     1621 gcagcgatca gaagatcgcg tggctgactg gcgagttggg ctttgacggc gctttcaatt
     1681 acaagaccac gcaagactac gcggccaagc tgaaagagct ttgcccgaag ggcatcgatt
     1741 gctatttcga caacgtcggc gggaacgtta ccgacgctgt gcttccagtg ttgaatgtac
     1801 gggcgcggat ttcggtgtgc gggcagattt cgcaatacaa cgcgaccaag cccgagttgg
     1861 gcgtgcgtcc atatattttt ttgctgagca aacagtgccg ggccgaaggt ttcatcgtca
     1921 cgcaattcat ggagcgttat ccggagggcg tggcgcagat ggcccagtgg atcaaagaag
     1981 gcaagctgaa atatcgcgag cacatcgtga acggattcga gaatgcgccg cgcgcgctga
     2041 ttggcgtgct gcaaggcgaa aacaccggga agatgctggt gaagttcgcg gacgcgtaag
     2101 cggaccatac gagggccgac agtgtacgcg gagttggcgg ccaaatccaa gagaggtcct
     2161 tcgactgtgt cgcgcgcccg cgaaaaacgc gggaaaagcc gaacgcgcgc gactccgctc
     2221 aggatgacga cggcaactgt gggccacatg cacaggcaag agtgcctgtg ccacgggcaa
     2281 cctacaccca ttcgccggcg cgcatcaccg gctctgaagt gccggacgcg ctgataccgt
     2341 ccacatcgat gcgattcgag ccgatcatcc agtcaacgtg aatcaggctt tcgtttgcac
     2401 ctttcgatgc gagttgttct ggggtgaggg tgtcgccatt tttcaggcag gtgctgtacg
     2461 cctgtccgag agcaatgtgg caggcggcgt tttcgtcgaa gagagtgttc atgaagagca
     2521 aaccgctggc cgcgatgggc gacgagtgcg ggactaaaga tacttcgccg aggcggcgcg
     2581 cgccttcgtc cgtctcgatc atgcgctgca ggacttgttc gccgcgcgcg gcgtgggcct
     2641 cgacaatttt gccggcctga aatcgcacgg aaatttcttc gatcatcgta ccctgatggg
     2701 agagcggctt ggtgctggtg acgcgcccat cgacgcgatc tttgtgaggc gtggtgaaaa
     2761 cttcttcggt aggcatattg ggaatgcaat agcgcccatt gcccgcggtt gtgcctccgc
     2821 cgagccatag gtgatcgtcg gccaggccca cgcggagatc ggtgcctggt ccgcggaagt
     2881 gcaaggcgga ataacgcttt tggttcagca tgtcggcgcg agcgtgcaaa tgcgcgtcgt
     2941 gcttcttcca tgcggcgagg ggatcgggct ggtcggcacg cgatgcggcg aagatggcgg
     3001 tccaaaggcg cgcgagggct tcgtcttgtg gaagatcggg aaacaccgcc gctgcccact
     3061 cgggtgtggc gcaggctatg atggtccaat tgatttcgtg gcgggtgatc agctccagcg
     3121 ccggacggta tgccttcgaa gttgcgcggt tcagccggct caccttttcc ggatcttctt
     3181 ttgagagcag cgaaggatta ttgccggtaa ttgccaggcg cgcagcgcca ttgcggtatg
     3241 cctgagccat gccttcgtag agccacgctg gagcgacgtc gaaactagca tctggagcgt
     3301 agcgaaaacg aagcagggcc gactgctcgt cagaaaacaa agtggtcacg agcgaggcgc
     3361 ccgctttgta ggcgtgctcg atgatgagtc gcgcgagagg gaccgagtcg agcgtggccg
     3421 tcatcacaag ctcctgtccc ggcgcgagtc ccagtccggc gcggatcgca acttcggcga
     3481 ggcggtcgag atttttttcg aattcgggag tgccggcaaa gggtcccttg gcggatactg
     3541 tagtcgaggt catggctagc att
//
LOCUS       DATNDT010000284         3397 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3182577, whole genome shotgun sequence.
ACCESSION   DATNDT010000284 DATNDT010000000
VERSION     DATNDT010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3182577"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /gene="murF"
                     /locus_tag="VN788_09135"
     CDS             complement(<1..981)
                     /gene="murF"
                     /locus_tag="VN788_09135"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HXU20730.1"
                     /translation="MRLPLFRIAEFLSATGEFDREAVATAYSIDSRTIEAGQLFFAVK
                     GDRFDGHDFVEQALQKGAVAAVVRQDSLPRYTNKDQLLAVDDTLFALQRLALAVRRLW
                     GKPVVGVTGSAGKTTTKEAIAHVLSKRFRVLKSEGNFNNHFGLPLMLLKLEPEHDIAV
                     IEMGMSHAGEITALAKIAEPEVGVVTNVAPVHLEFFDSIAGIARAKYELIASLPAHGT
                     AVLNADDEYVSQFGRDFKGKVLTYGLALSADVRAENIGSDGGLGSAFEVIAEGFRQRA
                     TLPLVGAHNIHNALAAVAVALDRGMSPTEAVNALSSLSPADKRGQVVKVGN"
     gene            complement(986..2500)
                     /locus_tag="VN788_09140"
     CDS             complement(986..2500)
                     /locus_tag="VN788_09140"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HXU20731.1"
                     /translation="MIFLELLDGAEVLAQSGNPQILGLEYDSRKVKTGYAFVAMRGEA
                     SNGNKFIDQAIGAGAVAIVSDSAAEKPRANVAWAQVAHGRRALARLSANFYRHPAERL
                     AVTGVTGTNGKSTTAFIVESILAAAGHKSALVGTIEYHVVGKVLPAPHTTPEALELNQ
                     IFAEAVSQSASEAVMEVSSHALAQERVFGIPFDVAIFTNLTRDHLDYHKTMDEYFAAK
                     QILFTGCGTEPPRVAVVNGDDEFGRQLAVFCKKQGSQVFTYGWASGDFFAKEIQITPQ
                     GTRFDLVTPGGTIPLFSPLIGKVNVYNILAAAGAAYARGCKAEAISKGITSLARVPGR
                     FERVDCKQPFTVVVDYAHTDDALRNLTALAREFVQQAGGKGRVITLFGCGGDRDRAKR
                     PLMGEAAGEGSDFVVLTSDNPRSEDPLAIINDALVGVQRSGVQYKVEPDRASAITLAI
                     QQAEAGDIVLLAGKGHEKTQILRTGPVPFDDLDAARRALRNAGYDCEVPDVNQA"
     gene            complement(2538..>3397)
                     /locus_tag="VN788_09145"
     CDS             complement(2538..>3397)
                     /locus_tag="VN788_09145"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXU20732.1"
                     /translation="ERRVVSPFTAAEMKQMMQGVILHGTGRKAVLEGYSAAGKTGTAQ
                     KIDPETKAYSHTKYWGSFAGFAPVNNPAIVVAVILDSAVGPHQGGQVAAPVFQSVAQQ
                     TLEYLHVPHDVELPQSRQLLLAEKRVKDKDLEEGSPDHLGDSVEVADANTTDSIQPRA
                     AENSHPVNGPEQSSGNNLVPASLTQRESWPETESASSAPSKETSAQQPSTKSAPIDSS
                     ATVVLDVAEGGIVVPSFIGKSVRSAIEVAEGSGLDLDAEGSGVARDQAPVAGSRVAAG
                     SRIVVRFGR"
BASE COUNT          695 a         1067 c          916 g          719 t
ORIGIN      
        1 gttacccacc ttgacgacct gcccgcgttt gtccgccgga ctgagtgaac tcaacgcatt
       61 caccgcttcc gtaggactca ttccacgatc aagcgccacc gccaccgccg ccaaagcgtt
      121 gtgaatattg tgcgctccca cgagcggcag ggtggcgcgt tggcgaaaac cctccgcaat
      181 gacttcaaag gccgaaccca aaccgccatc ggagccaata ttttccgcgc gcacgtccgc
      241 cgacagggcc aagccatagg tcaatacctt tcctttgaag tcgcggccaa attgcgaaac
      301 atattcgtca tcggcattga gcacggcagt gccgtgcgcg ggcagggaag ctatcagctc
      361 atatttcgcg cgggcgattc cggcgatgga gtcaaaaaat tctagatgca ccggcgcgac
      421 gttggtcacc acgccaactt ccggctccgc aattttggcc agcgcggtaa tttctcccgc
      481 gtgcgacatc cccatttcaa tcaccgcaat atcgtgctcc ggttccagtt tcaggagcat
      541 aagcggaagc ccgaagtggt tattaaaatt accctctgac ttcagcaccc ggaagcgctt
      601 gctcagaacg tgcgcaatgg cctctttcgt ggtggtcttg cccgccgatc cggtcactcc
      661 aacaaccggc ttgccccaaa gcctgcgcac cgcaagcgcc aaccgctgaa gggcaaagag
      721 cgtgtcgtcc acggcaagta actgatcttt gttggtatag cgcggcaggg aatcctggcg
      781 cacgacggcg gccacggcac ctttctgtag ggcctgctca acgaaatcat gtccgtcaaa
      841 gcggtcaccc ttgacggcga aaaaaagctg gcctgcctca atggtgcgcg agtcaatgga
      901 ataagccgtg gccacagcct cgcggtcgaa ctcgccagtt gccgaaagga attcggcgat
      961 gcgaaaaaga gggagcctca tacttttatg cttggtttac atccggaact tcacaatcat
     1021 accctgcatt tcgcagcgcc cgccgcgccg catccagatc atcaaagggc acgggcccgg
     1081 tgcgtagaat ctgcgtcttc tcatggccct ttccggcaag caaaacgatg tcgccagctt
     1141 cggcctgctg aattgccagt gtgatggcgc tggcgcggtc gggttccact ttgtattgca
     1201 ctcccgaacg ctgcacgccc accagagcgt cattgataat ggccagcgga tcttcgctgc
     1261 gcggattatc cgacgtgagc acgacaaagt cactgccttc gcccgcggct tctcccatca
     1321 gcggacgctt ggcgcgatca cggtcgcctc cgcagccgaa caatgtaata acgcgtcctt
     1381 tgccgcccgc ctgctgcacg aattcccgcg ccaaagctgt caggttgcgc aacgcatcat
     1441 cggtatgggc gtagtccacc acaacggtga acggctgctt gcaatccaca cgttcaaatc
     1501 gtcccggaac gcgggcaagg gaagtgatgc ctttggagat tgcttcggct ttgcatccgc
     1561 gagcgtaagc cgcgcctgct gccgccagaa tgttatagac attcaccttg ccaatcagcg
     1621 gagaaaataa gggaatagtt ccgcccggcg ttaccagatc aaagcgcgtg ccttgcggcg
     1681 tgatttgaat ttccttcgcg aaaaaatcgc cgctcgccca tccgtaagtg aatacctgcg
     1741 aaccctgctt cttgcagaag actgccagtt gccggccgaa ttcatcgtca ccattgacga
     1801 cggccacgcg cggaggttcg gtgccgcatc cggtgaacag aatttgcttg gcggcaaagt
     1861 actcgtccat ggttttgtga taatcgagat gatcgcgggt gagattggtg aaaatcgcga
     1921 catcaaaagg aattccgaac acccgctcct gcgccagcgc gtgggaggag acttccatca
     1981 cagcttcact cgcgctttgc gacaccgcct cggcgaaaat ctggttgagt tccagcgctt
     2041 ccggcgtcgt atgcggagcg ggcagaactt ttcctactac gtgatattca atcgtcccga
     2101 ccaaagcaga tttatgtccc gccgccgcga ggattgattc cacaataaac gccgtagtgc
     2161 ttttgccatt ggttccagta acgccggtca ctgccagccg ctccgctgga tggcgataaa
     2221 aattcgcgct caggcgcgcc agcgcgcggc gtccgtgggc aacctgcgcc caggcaacat
     2281 tcgcacgcgg cttttccgct gctgaatcgc tgacaatcgc gaccgctccc gcgccgatcg
     2341 cctgatcgat aaatttgttt ccattgctcg cttcgccgcg catggccacg aaggcataac
     2401 cagtcttgac cttacgcgaa tcgtattcga gcccgaggat ctgcggattg ccgctctggg
     2461 caaggacttc cgctccgtcg agtagctcca ggaagatcat gaaggtggat tatagagtag
     2521 caatctgccg cgccgatcta ccgtccaaag cggaccacaa ttctggaacc tgccgccact
     2581 cgcgatcccg ccaccggcgc ctgatcgcgg gccacgccgc tgccttcggc gtctaaatca
     2641 agtccgctgc cttctgcgac ttcaattgcc gagcgcacgc tttttccgat gaatgacggc
     2701 accacgattc caccctccgc cacgtcgagc actaccgttg ccgaagaatc aatcggcgca
     2761 gatttagtgg acggctgctg cgcagaggtt tccttgctcg gcgcgctgga tgcgctctcg
     2821 gtctccggcc acgactcgcg ttgagtcagc gacgcgggca ccaggttgtt cccggaactc
     2881 tgttcgggac cattcacagg atgagaattt tccgcggctc tgggctggat gctgtcggtc
     2941 gtgttcgcgt cggcgacttc cacgctatcg cccagatggt cgggcgatcc ttcttccagg
     3001 tctttgtcct taacgcgctt ttccgcaaga agaagctggc ggctctgcgg cagttccaca
     3061 tcgtgcggca cgtgcagata ctcaagtgtc tgctgggcga cgctttgaaa aacgggcgcg
     3121 gctacctgtc cgccctgatg cgggcccact gccgaatcca gaatcacagc aactacaatc
     3181 gccggattat tcacgggcgc gaagccggca aacgatcccc aatatttcgt gtgcgaatac
     3241 gccttggtct ccggatcaat cttctgcgcc gtgccggttt tgcccgccgc gctgtagcct
     3301 tcgagcacag cttttcttcc cgtgccgtgc aatatcacgc cctgcatcat ctgcttcatc
     3361 tcggctgcgg taaacggaga gacaacgcgc cgttcat
//
LOCUS       DATNDT010000285         4998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2595878, whole genome shotgun sequence.
ACCESSION   DATNDT010000285 DATNDT010000000
VERSION     DATNDT010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4998
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2595878"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            309..1649
                     /locus_tag="VN788_09150"
     CDS             309..1649
                     /locus_tag="VN788_09150"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HXU20733.1"
                     /translation="MIAWRYKVRMDKIENKDVRPVRLRQPDRSQVEMIFQCPDDLIPQ
                     DHEARIIWRVCESQDLLEFCQPIKAREGVCGRDSTTPVLLVSLWLYAAVRGIGSAREL
                     ARRCKESKPFQWLCGGVSINHHTLSDFRINHGPALDALFTRILAALVDKKLVKIYRIS
                     QDGLRVRACAGASSYRREERLQKLLEDATQHVEDLKKLLEDPEKSAGVSAKQKAARTR
                     AARERQQRVEQAINRLPDLKKKQEKLAKKKSAKEKQKLKEPRASTTDAEATVMKMANG
                     GFNPAVNVQFATDTESRAIVGVEVSGEGNDHHLAEPMREQVEQRTGQKVHEHLIDGGY
                     LVMDEIERAAAEDVTVYVPPKPPRNKAGYGSEYEPRPGESQVIQDWRQRMGSEAGQEI
                     YKQRASTSETVNADVRTHRGMGRMLVRGLKKARCVALWCALAYNVMHFGAALLA"
     gene            complement(1813..3153)
                     /locus_tag="VN788_09155"
     CDS             complement(1813..3153)
                     /locus_tag="VN788_09155"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HXU20734.1"
                     /translation="MRHYPNFRALIRVTFVAALALTGVGVGAEGGPATRSGPPRRFSG
                     VLSDETGKAMAGVRVVLVPGSPVITNEEFYEQQEGLPATTTGPDGHFSLPSPGEPQAL
                     VALTKEGYATAAVLAGSTADVALRVSPWCRIEGSVDVSGKAAGAGVEVVADWQGSVAD
                     VFFSSSTRTDAQGRFSFPRAFAGTYRVFRRGAHSENGRSIEVVAAAGKPAWAEVRAMG
                     RPVIGKIDLPAELRTRGNLRIEMESPEQYLDLPKVTLPPNVRTLSPEARLTWFRQWEQ
                     TPVGRQNSATWETAKRMMSSDEGPQLRREERTFYMLDAPAGRYMSVAQVFDPDRKTPE
                     PLAAFTYRFIIPPLPPGIMGTADELDLGVLAPAPQGAEPGDQPAPDIAFRGLDGKDHT
                     LSEFKGKVVLLDFWGVWCGACQLEIPRLKKLQAQFGGNPDFKLISLRACEVSPD"
     gene            complement(4702..>4998)
                     /locus_tag="VN788_09160"
     CDS             complement(4702..>4998)
                     /locus_tag="VN788_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20735.1"
                     /translation="DKGEIVYQKLLDADVYTTFRGGMGSSPTLAGKYIYLFGNQGVTL
                     VIEPGRTYKQVARNRVESCSPTSHGQETMESCPVFEGNRLYYRGSENLYCIEEK"
BASE COUNT         1102 a         1374 c         1537 g          985 t
ORIGIN      
        1 aacttcatcg atgcaacaca tgacccggtg aacgtttggg tggcattggt tggctcgtct
       61 tgtctgtgac aagtacaacg ccaattcgtc tcctgcacca ggttttttac ctgcaacttg
      121 ctccaccgct gccattacca accagcggcg cggaagttga tgcggttccg aaactcctgt
      181 gaagccgaaa taaccctcgg gaattgctct tgatgaggga aggcggtcga cgcctaggaa
      241 caggaccgaa ataacttgac atcgcctacc cgtcatcctg agagcctgtg aatttttggc
      301 gcgctttttt gatcgcctgg cgctataagg tacgcatgga caaaattgag aacaaggatg
      361 ttcgcccggt ccgccttcgc cagcctgatc gctcacaggt agagatgatc ttccagtgcc
      421 ccgacgatct gattccgcag gatcatgaag cacgcattat ctggcgggtc tgcgaatcac
      481 aggatctgtt ggagttttgt cagccgatca aggcacgcga gggagtctgt ggacgagatt
      541 ccaccactcc ggtcttattg gtgtcgttgt ggctgtacgc cgccgtgcga gggattggct
      601 cggcgcgtga gttggcgcgg cggtgcaagg agagcaagcc gttccagtgg ttgtgcggag
      661 gcgtgagcat caaccatcac acgctttcag acttccgcat taaccatggc ccggcgttgg
      721 acgcgttgtt cacgcgaatt ctcgcggcac tggtggacaa gaagctggtg aagatctatc
      781 gcatcagcca ggacggatta cgtgtacggg catgtgccgg ggcgtcgagc tatcgccggg
      841 aggagcgtct gcaaaagctg ctggaagatg cgacccagca cgtggaggac ctgaagaagc
      901 tgctggaaga cccggaaaaa tcggcgggag tgtcggcgaa acaaaaggca gctcggaccc
      961 gtgcggcgcg ggaacgccag cagcgcgtcg agcaggcgat caatcggctg ccagatctga
     1021 agaaaaagca ggaaaagctg gcaaagaaga agtcggcaaa ggagaagcaa aagctgaagg
     1081 agccgcgggc cagcaccacc gatgccgagg cgacagtgat gaaaatggcc aacggagggt
     1141 tcaatccggc ggtgaacgtg cagttcgcaa cggatacgga aagtcgggcg attgtgggcg
     1201 tcgaggtcag tggcgagggc aatgatcacc acctcgccga gccgatgcgt gagcaggtgg
     1261 aacaacgcac cggacagaag gtgcacgaac atctgataga cggcggctac ctggtgatgg
     1321 atgaaatcga gcgggccgcg gcggaggatg tgacggtcta tgtcccgccc aaaccgccgc
     1381 ggaacaaggc cgggtacggc agcgaatatg agccgcggcc aggagaaagc caggtcattc
     1441 aagactggcg gcagcgcatg gggagcgagg cgggccagga gatttacaag cagcgtgcat
     1501 cgacaagcga aactgtgaac gcagacgtgc ggacgcatcg cggcatggga cggatgctgg
     1561 tgcgtggatt gaaaaaggcc aggtgcgttg cgctgtggtg cgcgctggcg tataacgtga
     1621 tgcacttcgg tgccgcgttg ctcgcctgag cggcaaaaat tgccgttcgg aggcaaaaag
     1681 gcagcggcgc gcagccgcac tgacagatag cttcatggcg agggggactg acaagagtgc
     1741 cgcagtcatg ccgacggcag tcgatcggcg cggcgaaaga aaaaaaagag ccgtatggct
     1801 cagcggtaaa cctcaatccg gcgaaacttc acaagctctg aggctaatca gcttgaaatc
     1861 agggttcccc ccgaactgcg cctgaagctt cttgagtcgt gggatctcca gttggcacgc
     1921 gccgcaccag acaccccaaa agtccaacag cacaactttt cccttgaatt ccgagagcgt
     1981 gtgatccttg ccgtcgaggc cacgaaacgc gatgtccggc gccggctgat cgccgggctc
     2041 ggcgccctgc ggcgcaggag cgagtacgcc cagatccaat tcgtctgccg tacccattat
     2101 gccgggtggc agcggcggga tgatgaaccg gtaggtgaac gccgcgagtg gctcaggcgt
     2161 cttccgatcg gggtcgaaca cctgtgcgac ggacatgtac cggcccgccg gcgcgtcgag
     2221 catgtagaat gtcctctcct ccctccggag ctgcggcccc tcatccgacg acatcattcg
     2281 cttggccgtc tcccaggtgg cgctgttttg ccgccccacc ggcgtctgct cccattgacg
     2341 gaaccacgtc agccgcgcct ctggagacag agtccgcacg ttcggtggga gcgtgacctt
     2401 cggaaggtcg aggtattgct cgggcgactc catttcgatg cgcaggttgc cgcgagttcg
     2461 cagctctgcg ggcagatcga ttttcccaat caccgggcga cccatcgcgc ggacctccgc
     2521 ccacgccggt tttccggctg ctgcaaccac ttcgatggac cgtccgttct cgctgtgagc
     2581 tccgcggcgg aagacgcggt aagtgccggc gaacgcgcgc gggaaggaga agcggccttg
     2641 cgcgtccgtc ctcgtgctgg agctgaagaa cacgtcggca accgaaccct gccaatccgc
     2701 caccacttcc acgccggcgc cggcggcctt gcccgacacg tccacacttc cctcaatgcg
     2761 gcaccagggg gacacgcgca gcgcgacatc cgccgtggag ccggccaaga ccgcggcggt
     2821 ggcatatcct tccttcgtca gcgctacgag agcctggggc tcaccgggcg acgggagtga
     2881 gaagtggccg tcgggtcccg tagtcgtggc gggcaagcct tcttgttgtt cgtaaaactc
     2941 ttcgtttgtt atgactgggg aaccgggcac caggacgact cgaacgccag ccatagcctt
     3001 gcccgtctcg tcgctcagca cacctgagaa acggcgcggt ggccccgatc gcgtggcggg
     3061 gccgccttcc gccccaacgc ccaccccagt aagagccagg gcggctacaa aggtcactct
     3121 tatcagagcc cggaagttcg gatagtgcct catcagcgtc cttgttagac cggcgctgcc
     3181 ggaagcaaat cgtccaagat gcgcgaggcg cccagtgtac taagcggcta cgatatccgc
     3241 gagaaggatt tctcagggtt ttccgtggca acgaaaaggc gattaaggcg catggcggtg
     3301 ataagctggc acaagtcaag gcgtgcatcg aaaagtatga gattcggtat aaaggttccc
     3361 aaggggcaca acaagtcagg aggggagaaa taaccgtcgc gtctcctcgt cagttcaaac
     3421 tcattagtaa tgttcctggc ggtcacaatc tcaccgttca aatgaacagc gatacttgca
     3481 cgagcagtgt cgatggcaaa gaaaacgcgt ccgcctggtc gtcggtgact tcggtcgtgt
     3541 ctttcagcac cacgttgaat tcgtacgtct tcattgccgg ctccttcgaa atcgctggtc
     3601 gcacgttttc caccattgga agtgtcggac gcgacctcag ttcttcggtc ccgaaaaccc
     3661 atggggtaaa atttctgcct tcggccagat cagcacttac aagtaatttt gtccacattt
     3721 tgtactgcta tagcagggga aacgaatgac gaaggttcaa tgacaggcct caccggtcgg
     3781 cagcaggatg cctggaaagg cacgcttcgg gtggcgatgc accaaacctc gccgtgtgcg
     3841 aggcagatct ctattggatg gctcaatcaa ataattgctc tgccaattgt gaaaaatcgg
     3901 tcataatcgg tcacaatcgg tccttgaacc ccactggcga gctaaaaacg agggtgggtg
     3961 tggccaatcg gtcagcgatc ttcggccgat cgggtcgtga ttgcattgcg gggcggccgg
     4021 gtgcccgcgg aaggcgtaaa tccacactgc ggcgggtctt gcagaattcg tgaaaaaatt
     4081 tcgaaaattt ttgccaacaa aatattagtt cgtacgttat taggtatgaa cgctagaagc
     4141 atgtgagact taaagcggat cgagcgggaa aacaccggca ttggacctga cgatgccgct
     4201 accgaaagct atgggtcgaa tttttctatt ccttgcaggc acgaatctta caaatttaaa
     4261 tgtaatcttt ttggacatac tgtagaggga gagcattatg aagaaggtat tccatgtggc
     4321 acgggcggct cgcccgtgtt ttcagatgag caacacgggc gggccgcccg tgcctgaaga
     4381 tttcacggtc tctgagtgcg agccgatacc atgggtagat ataccggctg gcgcaacaca
     4441 aggacggggc gcgaaatttt cgaaaatgat gcgccatggg gcgcggacgg ttctgcagtg
     4501 ggtcgcgtgg tggttggaag tcttgtttca tgaaacgtat accgcgtcgg cccattcagc
     4561 ccccctcccc gggaacgcac aaacgggaca ggaacgcaca aacgggaccg tccaatatca
     4621 cgttttgtgc ggcgcctctt tgcagggtaa ctgaagttag ttggtcaacc gcgctactcg
     4681 atgaatgatc acacacccag attacttctc ttcgatgcag tagagattct ctgagccgcg
     4741 gtagtagaga cggttgcctt cgaatacggg gcaggactcc atggtttcct ggccgtggct
     4801 tgtgggagaa caggattcca cgcggttcct cgcgacctgt ttgtatgtcc gtcccggctc
     4861 gatcaccagc gtgacgccct gattcccgaa aaggtaaatg tacttgcctg cgagcgtggg
     4921 actggatccc atcccgccgc ggaaggtggt gtagacgtcg gcgtcgagca atttttggta
     4981 gacgatctct cctttgtc
//
LOCUS       DATNDT010000286         9288 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_16541, whole genome shotgun sequence.
ACCESSION   DATNDT010000286 DATNDT010000000
VERSION     DATNDT010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9288)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9288)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9288
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16541"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VN788_09165"
     CDS             complement(<1..1146)
                     /locus_tag="VN788_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXU20736.1"
                     /translation="MPDIKAIQSEMKASKVDGWLFCDHHHRDPIAARILGLDGYAMAT
                     RRWFYLIPSRGEPRKLVHRIESGMLDSLPGRKQLYSGWEELHKALGKLLGAGRTIAMQ
                     YSQENNIPYIGLVDAGTIELVRKLKKKVVTSADLVQKFEAVWTAEQLASHLEAGAILD
                     RIAQAAFERAASFVRDGRPITEYELQQWMLGRFHANELVTAEPPIAAVQPNNGNPHYE
                     PTASASRPIRAGDLLLLDIWAKCDRPGSVYYDITWTGYLGERVPDAYARIFRIVRDAR
                     NRAIEFVSDAVAHRRSIHGWEVDRVARETIRKAGYGKYFVHRTGHSIGQDVHGNGANM
                     DGLETRDDREIVPRTCFSIEPGIYLPEFGIRSEVNVFVSESEARVTGA"
     gene            1260..2546
                     /locus_tag="VN788_09170"
     CDS             1260..2546
                     /locus_tag="VN788_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008918542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU20737.1"
                     /translation="MPSDTTPVVERERLTPNQVRGFWASWGGWALDGMDSFIYALVLV
                     PALRELLPKSGIPATAGNVGFYGGLLFALFLVGWGLALLWGPVADKFGRVRTLMLTIL
                     CYSIFTFLAALGQNVWQVAICRLLAGVGIGGEWAMGGTFVAEEWPESRRKMGAALMHT
                     GYYFGFFLAAIANHFVGERHGWRPMFAIGGAPALLVGFILYSVKESDRWKSHVRKVSR
                     PIAELFKPEFRRRTWLNSAYVLISIIGLWAGAVYVPASVTEIAARQHMSGALAAQLAT
                     YATVILSFGTILGCLPLPWISDRIGRRATLAVYFVLMAISVGGSFGFAFYLSQNAMTW
                     FFVGLFFLGLGGANFAVYTLWLPEQYPTECRGSAFAFATSVGRFIGAGFTFLVGAGVS
                     HFHTIGIPVALTAIPFILGLFLIPFGVETTGQPLPD"
     gene            2646..3542
                     /locus_tag="VN788_09175"
     CDS             2646..3542
                     /locus_tag="VN788_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU20738.1"
                     /translation="MDAIALENVTKSYDAVTAVSGVNLQVREAAILGLLGPNGAGKTT
                     TIRMVMNILVPDEGAIRVLGKPVTDKTRESIGYLPEERGLYQRMKVRDLIVFLGALRG
                     LPEAEAGRRAGEWLERFGLGDWSEKKVADLSKGMQQKVQFITAIIHQPPLIILDEPFS
                     GLDPVNAGVVKDIMLEMRDRGATIVLSTHRMEQVEKLCDSICLIDHGHNVLDGELRAI
                     KQSYGKNTVHFEFSGPDGFLDHPSIATVNRFASGVEAKLHPGADPQQILKAAVDSGVQ
                     ITRFELLEPSLNDIFIEKTHHA"
     gene            3535..4803
                     /locus_tag="VN788_09180"
     CDS             3535..4803
                     /locus_tag="VN788_09180"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20739.1"
                     /translation="MRKTWLIVKREYLTRVKTKGFVIGTLLIPFIGIVSVALFAFLAS
                     HGSKDKFRIAIVDNIGGLAESVAQELDRPAKAGNPQFTVTQSIEEPLSSNEVEDDLRA
                     QVNSRTLDAYLVISADLSKPAQLHTKNPGNFRVIGPLTAAVNEALIQARLNARGIHVK
                     DLGDVTRAQELQVIKVSAGGEAVEKGQTVGVAIGLVILLYMSLLMYGIITMRSVLEEK
                     TTRTMEVLISSVQPFQLLTGKILGVAGVAFTQFLIWVASLGLVLSYGAVMAAMFNPGS
                     RFPAIHFPISLLLWSVFFFFGGYFLYSAMFAAIGAACSSEQDAAQLQWLAMGPLVFTM
                     MIYWMVLSDPSSKASVVLSEIPWFSPILMPLRISIQPPPLWHLVLSVVLLLLAILGVI
                     YVSAKIYRIGVLMYGKRPTLPELVRWLRYS"
     gene            complement(4800..5498)
                     /locus_tag="VN788_09185"
     CDS             complement(4800..5498)
                     /locus_tag="VN788_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HXU20740.1"
                     /translation="MTTLRDRLAVVEERIVSACRRAGRERSSVRLMAVSKTHPAEAIR
                     EAAEAGLTLFGENRVQEFESKQPKLADLLDATWHLIGHLQSNKARRAVHLFHQADSID
                     SLSLAQRLDSAAAAESKRLPVLIEMNLGGEATKSGAAEGDLHALAEGIEKMSHLELRG
                     LMTIPPYSDDPDAARSYFRKLRKVRKALSEQLGRTLLTLSMGMSHDFEVAIEEGATEI
                     RIGTALFGERDQRV"
     gene            complement(5526..6548)
                     /gene="glk"
                     /locus_tag="VN788_09190"
     CDS             complement(5526..6548)
                     /gene="glk"
                     /locus_tag="VN788_09190"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HXU20741.1"
                     /translation="MILAGDVGGTKVHLALYGFEHGKLLHVRDEKFPSREYAGLEEII
                     RNFLIESGDPDVTAACFGVPGPVRGGRLKLTNLPWVVDSRELSKALDINHLFLINDLE
                     ANGYGIAELDPEQLFELSAGAPGSVGNEGLVSAGTGLGEAILVWNGKMHIPMASEGGH
                     CDFSPRNEEEIELLRYLQKKLKGRVSFERVVSGQGLTNVYTFLRDVKKMEEPDWLRER
                     MLAEDPNAIIGELGQEGSNELCVKALDMFVSAYGAEAGNLALKVLSVGGIYLGGGIAP
                     KILKKMRDGAFMKAFSDKGRLSDLLVQMPVRVILESRCALMGAAAYADARAAELSGES
                     VRAASL"
     gene            complement(6545..7342)
                     /gene="pgl"
                     /locus_tag="VN788_09195"
     CDS             complement(6545..7342)
                     /gene="pgl"
                     /locus_tag="VN788_09195"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HXU20742.1"
                     /translation="MPRKTEVEYRVYDGADALSRAAAEHFLETTQAAVATHGVARVAV
                     SGGNTPKRTFELLANPAEKFATAMPWAQMELYWVDERMVPPDNKDSNYHMTREALLNK
                     VPLDPKRVFRIEGELDPEQAAARYESAIRNHLRLEGAEGPRFDVLALGMGDDGHTASL
                     FPHTEAINEMIRIAVANHVPQKDTWRVTLTWPVINEAYDVFFLIAGGDKAQPVHDVLL
                     GKYDPEALPSQLIRPRNGKLLMLLDREAAALLPPPDANGTGRLEIER"
     gene            complement(7356..8900)
                     /gene="zwf"
                     /locus_tag="VN788_09200"
     CDS             complement(7356..8900)
                     /gene="zwf"
                     /locus_tag="VN788_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXU20743.1"
                     /translation="MAAAAITVSPEDVAQAARSNERIPEPCVVVIFGASGDLTKRKLL
                     PALFHLEQAGLLPEQFGIVGVARRDLGVTFAEDMKKGIVEFGGVKDGADKLDEFIGKV
                     SYHAMNFDDDGGYTGLKSQLEKYDQQHGTKGNRLFYLAVAPEYFSDIIHRLGDHGMAH
                     PDKGNVRVIIEKPFGHDLESARELNDDVNKVFDENQIFRIDHYLGKETVQNILVFRFA
                     NGIFEPIWNRNYIDHVQITAAESIGIEGRGPFYEKAGALRDVVQNHMMELLSFVCMEP
                     PVSFEADAVRYEKVKVWRAIKPVDCASTVRGQYGSGTVDGKQVKGYREEDRVDPRSNT
                     ETFAAIKLEIENWRWAGVPFYLRAGKRLKKRATEITIQFKQPPMLLFADRGNRGSCAE
                     IQPNLITMRIQPDEGISLRFGAKVPSPDMTVCPVNMDFNYAAAFGTSSANGYERLLLD
                     AMLGDATLFAHRDGVEATWALYTPVLEAWAAAQPKNFPNYDSGSWGPKQADELMTRDG
                     RRWQKL"
     gene            complement(8947..>9288)
                     /locus_tag="VN788_09205"
     CDS             complement(8947..>9288)
                     /locus_tag="VN788_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate dehydrogenase"
                     /protein_id="HXU20744.1"
                     /translation="EGFAIMGQKKQMNLDLAQIAEIWRYGSVVRSWLLDLSADALKKN
                     PTLEGLEPYVADSGEGRWTVAEAIDLNVSAPVITESLIRRIRSRETNNFSDRMLAIMR
                     NEFGGHAVKKD"
BASE COUNT         1774 a         2908 c         2503 g         2103 t
ORIGIN      
        1 agcgccggtc acgcgcgctt cgctttcgct cacgaaaaca ttcacttcgc tgcgaattcc
       61 gaactccgga agatagatgc ctggctcgat cgaaaagcag gttcgcggga cgatttcccg
      121 gtcgtcgcgt gtttcgaggc cgtccatgtt cgcgccgttg ccgtgaacgt cttgcccgat
      181 gctatggccg gtgcgatgca cgaaatattt tccgtaaccg gctttgcgaa tggtctcgcg
      241 cgccacgcga tccacttccc aaccgtgaat cgagcgccgg tgcgccacag cgtcgctcac
      301 gaactcgatg gcgcgatttc tagcgtcccg cacgatgcga aaaatcctcg catacgcgtc
      361 cgggacgcgc tcgcccagat agcccgtcca ggtgatgtcg taataaacgc tgcccggccg
      421 atcgcatttg gcccagatgt cgagtagcag caaatcgccc gcacgaatcg gccgcgatgc
      481 gctggccgtc ggctcgtagt gagggttccc attatttggc tggacggcgg caatgggagg
      541 ctcagcggtg acgagttcgt ttgcgtgaaa acgtccgagc atccactgtt gcagctcgta
      601 ttcggtgatt ggccgcccgt cgcgcacgaa agatgccgca cgctcgaacg ccgcctgcgc
      661 gatgcggtcc aggattgctc cggcctccag atgcgatgcc agttgctctg ctgtccacac
      721 cgcttcgaat ttctgcacca aatccgcaga cgtaaccact tttttcttca gtttccgcac
      781 cagctcgatc gtgcccgcat ccacgaggcc gatatagggg atgttatttt cctgggaata
      841 ctgcatggca atcgtgcggc cggcgccgag caatttgcct agcgctttat gcagctcctc
      901 ccatccggaa taaagctgtt tgcggcccgg cagggaatcg agcataccgc tctcgatccg
      961 gtgcaccagc tttcgcggtt ctccgcgcga ggggattaaa tagaaccacc gccgcgtcgc
     1021 cattgcatat ccgtcaaggc caaggattcg cgccgcaatg ggatcgcgat ggtggtgatc
     1081 gcagaaaagc cagccgtcga ccttgctggc tttcatctcc gactggatcg ctttgatatc
     1141 gggcatggga atattttcgc cggccacgat tctaacagcc gagacaggcg cgttcaaatt
     1201 cgcagccagc gcgggaaacg ccttgcaacc tcctcgcact gttcgtaaaa tgcgtagcct
     1261 tgccttccga taccacgcca gtcgtcgaac gcgaacgtct cactccgaac caggtgcgcg
     1321 gattctgggc ctcgtgggga ggctgggcgc tggacggcat ggattctttc atttacgcgc
     1381 tcgtcctcgt gcccgcactt cgcgaacttc tgcctaaatc cgggattccc gccactgcag
     1441 gcaatgttgg cttttatggc ggccttcttt tcgcgctgtt tctggtcggc tggggattgg
     1501 cccttctttg gggacccgtc gccgataaat tcgggcgcgt gcgcacgctg atgctcacta
     1561 tcctttgcta ctcgattttc acttttctcg ccgccttggg acaaaatgtg tggcaggtgg
     1621 cgatttgccg cctgctcgcc ggagtcggca tcggcgggga atgggccatg ggcggcacgt
     1681 tcgttgccga agagtggcct gaatcgcgcc gcaagatggg tgctgcgttg atgcatacgg
     1741 gctactactt cggcttcttt ctcgctgcaa tcgccaacca tttcgtcgga gagcgacacg
     1801 gctggcgccc gatgttcgcc atcggcggcg ctcccgcgct gcttgtcggg ttcattcttt
     1861 acagtgtcaa ggaatcggac cgctggaaat cgcacgtccg caaagtttcg aggcctatcg
     1921 ccgagttgtt caagcctgaa ttccgccgcc gcacatggct gaactccgcc tacgtgctga
     1981 tttcgattat tggtctttgg gcgggcgcgg tctacgtccc ggcatcggtt accgaaatcg
     2041 cggcacgcca gcatatgtcc ggcgctctcg ccgcccaatt ggccacgtat gccactgtga
     2101 tcctctcttt cggcacaatc ctcggatgtc tccctttgcc gtggatctca gaccgaatcg
     2161 gacgccgcgc gacgctcgcc gtttattttg tcctcatggc gatatccgtt ggaggcagct
     2221 tcggcttcgc tttttacctg tcgcagaacg cgatgacatg gttcttcgtg ggattgtttt
     2281 ttctgggcct cggaggagcg aatttcgccg tgtacacgct ctggcttccc gaacaatacc
     2341 cgacggaatg tcgcggcagc gcctttgcat ttgccacctc ggtcggccgt ttcatcggcg
     2401 cgggctttac atttctggtt ggcgcgggag tgtcgcactt ccacaccatt ggcattcctg
     2461 tcgcgcttac cgcgattccc ttcattctcg gattgtttct gatcccattc ggcgtcgaaa
     2521 ccaccggcca gccgcttccg gattagccct cgattgcgat tttcttggca caggcccgta
     2581 acattatttt catctggcac gtagcattca gttaaagttt tgtatcgggg aacaaggcca
     2641 aatctgtgga cgccatcgct ctcgaaaatg tcaccaagtc atatgacgcc gtcaccgccg
     2701 ttagcggcgt gaatctgcag gtacgcgagg ctgccatttt gggcctactt gggccaaacg
     2761 gcgccggtaa gaccaccacc atccgaatgg tgatgaatat tctggttcca gacgaaggcg
     2821 cgattcgcgt cctcggcaag cctgtaaccg ataaaacgcg cgaatccatc ggttacctgc
     2881 cggaagagcg cgggctttat caacggatga aggtgcggga tttgattgtg tttctcgggg
     2941 cactgcgcgg ccttcccgag gccgaagccg gtcgtcgggc cggcgaatgg ctcgagcgct
     3001 tcgggcttgg ggactggtcc gaaaagaaag tggccgatct ctcaaagggc atgcagcaga
     3061 aagtgcaatt catcaccgcg atcatccatc agccgccgct aattattttg gacgagccgt
     3121 tttcaggcct cgatcctgta aatgccgggg tggtgaaaga catcatgctg gagatgcgcg
     3181 atcgcggcgc gacgatcgtt ctttccacgc accgcatgga gcaggtggaa aagctctgcg
     3241 attccatatg cctgatcgat cacggccaca atgttctcga cggcgagctc cgagcgatca
     3301 agcagtccta cggaaaaaat acggtgcatt tcgagttcag cggacccgac ggttttctgg
     3361 accatccttc gatcgccacg gtcaaccgct ttgcctccgg agtggaagcc aagctgcatc
     3421 ctggcgccga cccccagcag attctgaaag cggccgtgga ttccggcgtg caaatcacgc
     3481 gcttcgagct gctcgagccc tcgctcaacg atatcttcat cgagaaaaca caccatgcgt
     3541 aaaacgtggc tcatcgtcaa gcgcgagtat ttgacccgcg tgaagaccaa gggctttgtg
     3601 atcggtacgc tgctgatacc atttattggc atcgtatcgg ttgcgctgtt tgcattcctt
     3661 gccagccatg gctcgaagga caagttccgc atcgccattg tggacaatat cggcggactc
     3721 gccgagtccg tcgcgcagga gctcgatcgc ccggccaagg ccggcaaccc ccaattcacg
     3781 gttacccagt ccatcgaaga gccgctttcg tccaatgagg tggaggatga tttgcgcgcc
     3841 caggtaaatt cccgcacgct cgatgcctac ctggtgatct ccgcggactt gagcaaacca
     3901 gcccagcttc acaccaagaa tcccggaaac ttccgcgtga tcgggccact caccgccgcc
     3961 gttaatgaag cattgatcca agcgcgcctg aacgcgcgcg ggattcatgt gaaagacctg
     4021 ggcgatgtaa cccgcgccca ggagctgcag gtgatcaagg tcagcgcggg cggggaggcg
     4081 gttgaaaaag gccagaccgt tggcgtggcc atcgggctgg ttattctgct ctacatgtcg
     4141 ttgctgatgt acggcatcat taccatgcgc tcggtactcg aggaaaagac gacgcgcacg
     4201 atggaggtgc ttatttcctc cgtgcagcca tttcagcttc ttacggggaa aattctcggc
     4261 gttgcaggag tggcgttcac tcaattcctg atctgggttg cgtcgctcgg cttggtttta
     4321 tcctatggcg ccgtgatggc ggcgatgttc aaccccggtt ccagatttcc ggcgattcac
     4381 tttcctatct cgctgctgct ctggtccgtg ttctttttct ttggcggtta ctttctttat
     4441 tcagcgatgt tcgccgcgat tggcgcggca tgttccagcg agcaggatgc cgcccaattg
     4501 cagtggctgg ccatggggcc gctggttttc accatgatga tctattggat ggttctgagc
     4561 gacccctcgt ccaaggcatc tgtggtgctt tccgagatcc cgtggttttc accgattcta
     4621 atgccgttgc gcatctcgat tcagccgccg ccgctttggc atttggttct ctcggtggtg
     4681 ctgttgctgc tggcaatcct gggtgtgatt tatgtttcgg cgaaaattta ccgcatcggc
     4741 gtcctgatgt atggcaaacg cccgacgctg cccgaactgg tccgctggct ccgttattct
     4801 tagacgcgct ggtcgcgctc gccgaagagg gccgtgccga tgcgaatctc cgtagcgcct
     4861 tcctcgatgg ctacctcgaa atcgtggctc atgcccatgg agagcgtaag taaagtgcgt
     4921 ccgagctgtt cgctgagggc ctttcgcact ttccgaagct ttcgaaaata agaacgcgcg
     4981 gcgtcgggat catcgctata tggcgggatc gtcatcaggc cgcgcaattc caagtgcgac
     5041 atcttttcga taccttccgc gagcgcatgc aaatctcctt ctgccgcgcc gctcttcgtc
     5101 gcttcaccgc ccaggttcat ttcgataagg acgggcaatc gcttcgactc cgccgccgca
     5161 gcggaatcga gtctttgcgc gagtgacagg ctatcgatcg aatccgcttg gtgaaataga
     5221 tggacggcgc gccgtgcctt gttgctttgc aagtggccga tcagatgcca cgtggcatcg
     5281 aggagatccg caagcttcgg ctgcttcgac tcaaactcct gcacgcgatt ctcgccgaac
     5341 agcgtgagtc ccgcctcggc tgcctcgcga atcgcctccg ccggatgcgt ctttgaaacc
     5401 gccatcagcc gcacgctgct gcgctcccgg ccggcgcggc ggcaagcaga gacgatacgt
     5461 tcctcgacca cagccagccg gtccctgagt gtcgtcattc cctcatctta cccgcgcttt
     5521 ttgagctaca gcgaagccgc gcgaacggac tctccgctta actcagcggc gcgagcgtca
     5581 gcatacgcgg cggcgcccat cagcgcgcag cggctttcca gaatcacgcg aaccggcatc
     5641 tgcaccagca gatcgctcaa gcggcccttg tcgctaaatg ccttcataaa cgcgccatcg
     5701 cgcattttct tcagaatttt gggggcgata ccgccgccaa ggtaaatgcc gcccacggac
     5761 aataccttca gcgccaggtt tccggcctct gcgccatagg cggatacaaa catatccaaa
     5821 gccttcacgc agagctcatt cgagccttcc tggcccagtt cgccgataat cgcattcgga
     5881 tcttcagcca acattcgttc ccgcagccag tccggctctt ccatcttctt cacgtcgcgc
     5941 aggaaggtat acacattcgt cagcccctgc ccggaaacca cgcgctcaaa gctcacgcgg
     6001 cccttcagct tcttctggag ataccgcagc agctcaatct cctcctcatt gcgcggagaa
     6061 aaatcgcagt gcccgccttc ggaagccatg ggaatatgca tcttgccgtt ccaaaccaga
     6121 atcgcctcgc ccagcccggt gcccgcggaa accagcccct cgtttccgac cgagccgggc
     6181 gcgcccgcac tcagctcaaa caactgctcg ggatcgagtt cggcaatgcc gtaaccgttc
     6241 gcttcaagat cgttgatgag aaacaggtga ttgatgtcca gcgctttgga aagctcgcgg
     6301 ctgtcaacca cccagggcaa attggtcagc ttcagccggc cgccgcgtac cggtcccggc
     6361 actccgaagc aggccgccgt tacatcaggg tcgccgctct caatcagaaa attccggata
     6421 atctcttcca ggcccgcgta ttcgcgcgag gggaactttt cgtcgcgcac gtgcaaaagt
     6481 tttccatgct cgaacccgta cagcgccaga tgaaccttgg ttccacccac atctccagca
     6541 agaatcatcg ctctatctcc agtcggcctg tgccgtttgc atccggcggc ggcaatagcg
     6601 ccgcagcctc gcgatcgagc aacatcagga gtttcccatt ccgcggacga atcaactgcg
     6661 acggcagcgc ctctggatca tacttgccga gcagcacgtc gtgcacaggc tgcgccttgt
     6721 ccccacccgc aatcaggaaa aaaacgtcgt acgcttcgtt gattaccggc caggtcagcg
     6781 tcacccgcca tgtgtccttt tgtggaacat gattggcaac cgcaattcga atcatctcgt
     6841 tgatggcctc tgtgtgcgga aacagcgaag ccgtgtgtcc atcatctccc attcccagcg
     6901 ccagcacatc aaagcgcggc ccttccgcgc cttccagccg aagatggttg cgaatcgccg
     6961 actcataccg cgccgccgcc tgctcaggat ccagctctcc ctcgattcga aaaacgcgct
     7021 tcggatccag cggaacttta ttcagcaagg cctcccgcgt catatggtaa ttgctgtctt
     7081 tgttatcggg aggaaccatc cgctcatcca cccagtagag ttccatctgc gcccacggca
     7141 ttgccgttgc gaacttctcc gccggattcg ccagcaactc aaaagtgcgc ttcggagtat
     7201 tcccgccgga gaccgcgaca cgcgccactc catgggtggc caccgcagcc tgcgtagttt
     7261 cgagaaaatg ttcggcagcc gcgcgactca gcgcgtccgc tccgtcatac acccgatact
     7321 caacctctgt ttttctgggc atcctgtttt tatcttcaca acttttgcca gcgccgtcca
     7381 tcgcgcgtca tcagttcatc ggcttgtttc ggcccccagg agccgctgtc ataatttgga
     7441 aaattcttcg gctgcgccgc ggcccaggct tccagcaccg gagtgtagag cgcccatgta
     7501 gcctccacgc cgtcgcggtg cgcaaacagc gtggcgtcgc caagcatcgc gtccagcaac
     7561 agccgctcat agccattggc cgaggaggtc ccaaacgcag cagcgtaatt aaagtccata
     7621 ttcaccgggc aaaccgtcat gtccggactc gggactttcg cgccaaagcg caacgaaatc
     7681 ccctcatccg gctgaatgcg catggtgatc aggttcggct gaatctccgc acaggagccg
     7741 cggttgccgc gatcagcaaa aagcagcatg ggcggctgct tgaactgaat ggtgatttcc
     7801 gtcgcccgct tcttcaggcg cttgcccgcg cgcaggtaaa acggcacgcc cgcccaccgc
     7861 cagttctcga tttcgagctt gatggccgcg aaggtttcgg tgttcgaacg cggatcgacg
     7921 cgatcctcct cgcgatatcc cttcacctgc ttgccgtcta ccgttcccga accatactgg
     7981 ccgcgaaccg tgctcgcgca atcgacaggc ttgattgccc tccacacttt gactttttcg
     8041 tagcgcaccg catccgcctc aaaggaaacc ggcggctcca tgcaaacaaa tgaaagtagt
     8101 tccatcatgt ggttctgcac cacgtcgcgc agcgctccgg ctttctcgta aaacggcccg
     8161 cggccttcaa tgccaatgct ctccgccgcc gtaatctgca cgtggtcaat gtagttgcgg
     8221 ttccagatgg gctcaaaaat cccgttggca aatcgaaaca ccaggatgtt ctgcaccgtc
     8281 tccttgccca ggtagtggtc gatgcggaag atctggttct cgtcgaaaac tttgttcaca
     8341 tcgtcattca gttcgcgcgc actctccagg tcatgcccga acggcttttc aataatgacg
     8401 cggacatttc ccttatccgg atgcgccatt ccgtgatcgc caagccgatg gatgatgtcg
     8461 gagaaatatt ccggagcaac cgcaaggtag aacagcctgt ttcctttggt cccgtgctgc
     8521 tggtcatatt tttcaagctg tgacttcaat ccggtgtagc cgccgtcatc atcaaaattc
     8581 atcgcgtgat agctgacctt gccaatgaat tcgtccagct tgtcggctcc gtccttcacg
     8641 cctccaaact cgactatgcc ttttttcatg tcctcggcaa acgtcacccc caggtcgcgc
     8701 cgcgccacgc cgacaattcc gaactgctcc ggcagaagac cggcctgttc cagatgaaac
     8761 agcgccggaa gcagcttgcg cttggtcagg tcgccagagg ccccaaagat gaccaccacg
     8821 cacggctcag ggatgcgttc gttgcttcgc gccgcctggg ctacatcctc aggcgaaact
     8881 gtaatcgctg cggctgccat attgctcctt cattgctgat cggccttctg cggcgctgtt
     8941 gttggtttaa tctttcttga cggcgtgccc accgaactca ttccgcatga tggccagcat
     9001 gcggtcagag aaattgttcg tctcgcgcga ccggatgcgc cgaatcagag attccgtaat
     9061 cacaggcgcc gaaacattca ggtcaatggc ttcggccacc gtccagcgcc cttcgccgga
     9121 atccgcaaca tacggctcca acccttcaag cgttggattc ttcttcagcg catcggcgga
     9181 aaggtccagc agccacgatc gcaccacact gccgtagcgc catatctccg caatctgcgc
     9241 cagatccaga ttcatctgct tcttctggcc catgatagcg aagccctc
//
LOCUS       DATNDT010000287         3510 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4052340, whole genome shotgun sequence.
ACCESSION   DATNDT010000287 DATNDT010000000
VERSION     DATNDT010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3510
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4052340"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..986)
                     /locus_tag="VN788_09210"
     CDS             complement(69..986)
                     /locus_tag="VN788_09210"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXU20745.1"
                     /translation="MPFPGSPFSGVFAAVITPIDYSGNIDFATFDRVVDFVMDRGVDG
                     IVIGGATAEYVHFSVKDRSDLAAYAVRRMNGRGKVIAGVGASSIYSTLQLARCAEDAG
                     SDLLLIPMPYFFRYEQQDLAAYAEAVANSVSAPCLLYNLPSFTNGLEVDTAIRLLESV
                     PNLIGMKDSSGNRANLDPLAHARKNRNFSLFVGDDDLLLAALQAGWDGVVSGIACFMP
                     ELIRALYRSFREGYEDRALSYQKSLGELIEHVVRLPIPWGVRVGLAVRGIPNGPMHLP
                     PSPGRLRQMEELREWLNRWSARSVTELSK"
     gene            complement(1115..1729)
                     /locus_tag="VN788_09215"
     CDS             complement(1115..1729)
                     /locus_tag="VN788_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006678283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1349 domain-containing protein"
                     /protein_id="HXU20746.1"
                     /translation="MPNCLVHETFQETRLHSRLQWLNPPPVWRIDPTLPALVVEPQAE
                     TDFWQRTHYGFQADNGHLLYAEILGNTTITAKIHSYPVHQYDQAGLMVRFSPECWLKS
                     SVEFETNAPSQLGAVVTNGGYSDWSLQDFPYGPGLSCCLRIQRDGGDFLAEHAVSENG
                     PWFLMRVAHLFAEAEQPALAGFYACSPKGPGSRAALEWLKIEPH"
     gene            1807..2580
                     /locus_tag="VN788_09220"
     CDS             1807..2580
                     /locus_tag="VN788_09220"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HXU20747.1"
                     /translation="MPAKPVQKASERYFSRAIGNALRILEIFQQSRRSLTLSEVTQQA
                     NLPKSSAFRILRTLEISGYLRRVESDRFALAPSAAGIPNQSTVHVLEAAQAWMRRLSQ
                     EFRETISLAFLFENHIEVIAVIDSPHRISMGNMIGGLIPPHASSLGKCIAAFQAEDRA
                     EKLLRSFSLVRFTPGTIVEEMALHKELALVRARSYATDFEESTLGGCCLSAPIFGPDG
                     LAIAAISISMPKMRFTNQERLIAAVKDAAAAISGELRGA"
     gene            2666..>3510
                     /locus_tag="VN788_09225"
     CDS             2666..>3510
                     /locus_tag="VN788_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20748.1"
                     /translation="MKRRQFLQRAAVAVAAPGLVSFAAGPNGETIDLKNAAIITPREM
                     SPRANKAVVVLVEEAEKRCGIRWSVLTGSPPPGATAIHVDLQTTPKIAPEGYSIRSSA
                     GAQGRSIAISGTDDRGLLFGVGKFLRTVAFGRQQASVDARRLNVTSSPKYPLRGHQLG
                     YRPKTNSYDAWTVPMWDQYIRDLAVFGTNAIELLPPRTDDLPDSPHFPLPPGRMMREM
                     SRLADGYGLDVWVWYPAMDKDYSDPRTVDLAIKEWAATFQLLPRLDAIFVPGGDPGRT
                     APKYLL"
BASE COUNT          806 a          943 c         1033 g          728 t
ORIGIN      
        1 aatatccaca agcggtttgc cttcgagtct tgtcgactta taccgagccg gaataactcc
       61 aatagttttc atttactaag ttccgtcaca gaacgcgcgg accagcggtt aagccattcc
      121 cgcaattcct ccatctgacg caagcggccg ggagatggcg gaagatgcat cgggccgttg
      181 ggaattcccc gcacggccaa ccccacgcga acgccccagg gaatgggtaa gcgcacgaca
      241 tgctcgatta attcacccag ggatttctga taactcagag cccggtcttc atagccttcg
      301 cgaaaactgc gataaagcgc gcgaattaac tctggcatga aacaagcaat gccggaaacc
      361 actccatccc agcccgcctg caaagcggca agcagtagat cgtcatcacc tacaaagagc
      421 gaaaaattcc tgttctttcg cgcgtgggcc aggggatcga gattcgcgcg gttcccgctg
      481 ctgtctttca tcccgataag atttggcacg ctttcgagca gacgaatcgc ggtgtctact
      541 tcgaggccgt tcgtaaatga cggcagattg tagagcagac agggcgctga aaccgagttg
      601 gccaccgctt cagcgtaagc ggccagatcc tgctgctcat aacgaaagaa atatggcatg
      661 ggaatgagga gcaggtcgct gccggcgtcc tcggcgcagc gggctaactg aagagtggag
      721 tatatagagg aagcgccgac accggcgatc actttgccac gtccgttcat tcgacgcacg
      781 gcataggccg cgaggtccga ccgatccttc acgctgaagt gtacatactc ggctgtcgcg
      841 ccgccaatta caatgccatc gacgcctcgg tccatgacaa aatcgacgac acggtcgaaa
      901 gtcgcgaaat cgatattccc tgaatagtca atcggagtga tcacggcggc gaagacgccc
      961 gagaatggag agcctggaaa tggcattgaa tgcgctagat ctcgcccaat ctagcacagg
     1021 cgccttattc ggtcaatcaa aataaaactt gattttatat agaaaacgac atcggcctaa
     1081 atggaattgt agtatgatga tggatcgcga ggacctagtg gggttcgatc ttcagccatt
     1141 cgagcgcggc gcgggagcca ggtcctttgg ggctgcaggc gtaaaatccc gccaatgccg
     1201 gttgttccgc ttcggcaaac agatgagcaa cgcgcatgag aaaccagggg ccgttttcgg
     1261 atacagcgtg ttcggcgaga aaatcaccgc catcgcgttg aatgcgcaaa cagcaggaaa
     1321 ggccgggccc gtatggaaaa tcttgaagag accaatcgga gtacccgccg ttggtgacca
     1381 ccgcgccgag ctggctaggg gcattcgttt cgaactcgac ggagcttttc aaccagcact
     1441 ccggggaaaa gcgcaccatg agcccggcct ggtcgtattg gtgaacggga tatgaatgga
     1501 ttttcgcggt gatggttgtg ttgcccaaaa tttccgcgta caaaagatgg ccgttatcgg
     1561 cctgaaagcc atagtgagtc cgctgccaga aatcggtttc agcttgcggc tcgacgacca
     1621 aggccgggag agtcggatcg atccgccaga cgggtggcgg attcaaccac tggagacgag
     1681 aatggaggcg ggtttcctga aaagtttcgt gaacgagaca gttcggcata cgtttgtgcc
     1741 gtcatcatcg cacatgccgc atggcatggg ggttagtttc tgactaagac tagtaagaaa
     1801 gcggcaatgc ctgcaaagcc ggttcagaag gcctccgaac gctatttttc cagagcgatc
     1861 ggcaatgcgt tgcgcattct ggaaatattt cagcaaagcc gtcgttctct tactctgagc
     1921 gaggtcacgc agcaggcgaa ccttcccaaa agctccgcat tccgcatcct gaggacgctt
     1981 gagatcagcg gatacctgcg gcgggtggag agcgatcggt ttgcgcttgc gccatccgcc
     2041 gctgggattc cgaatcagtc gacggtgcat gtgttggaag cggcgcaagc gtggatgcgc
     2101 cggctcagcc aggaattccg cgagacgatc agcctcgctt tcctgttcga aaatcacatt
     2161 gaagtgatcg cggtcattga cagtccacat cgcatcagca tgggtaatat gatcggcggg
     2221 ctgattccgc cacatgccag ttcgctaggg aaatgtatcg cggcgtttca ggcggaggat
     2281 cgcgcggaga aactgctgcg gtcgttcagc ctggtgcggt ttacgccggg tactatcgtc
     2341 gaagaaatgg cattacacaa ggaactggca ttggtgagag cgcgaagtta tgccactgat
     2401 ttcgaagaga gcacgctggg cgggtgctgt ctgagcgctc ccattttcgg gccggatggg
     2461 cttgccatcg ccgccattag tatctcgatg cccaagatgc gattcacgaa tcaagaacgc
     2521 ctgatcgcgg cagtgaagga tgcggcggcg gcaattagcg gcgaactacg tggcgcttga
     2581 tgtcgccatg cctgttttag cggcaaaacg aaataccata ttcgtttgcg cccgttttca
     2641 ggttttgata aagtaagcgg cgccaatgaa gagaagacaa ttcctgcaac gggccgcggt
     2701 tgctgtagcc gctcctggtc tcgtgtcgtt tgcggcggga ccgaacggcg aaacgatcga
     2761 tctgaagaac gccgcgatta tcacgccgcg tgaaatgagc ccacgcgcga acaaagcggt
     2821 cgtggtactc gtggaagagg cagagaagcg ttgtggcatc cgatggtccg tgctaaccgg
     2881 atcgccccct ccgggcgcga ctgccattca cgtcgatttg caaaccacgc cgaaaattgc
     2941 gccggaaggt tattcgatcc ggagttccgc cggcgcacag ggccgttcga tcgcaatctc
     3001 gggaacggac gatcgcggcc tgttgttcgg cgtcggaaag tttcttcgca ccgtggcttt
     3061 tggcagacaa caggcatccg tcgatgcccg gcgcctgaac gtgaccagca gtccgaagta
     3121 tccgctgcgc gggcaccagc tcggatatcg tccaaagacg aactcctacg atgcgtggac
     3181 ggttcccatg tgggatcaat acatccgcga tctggcggtt tttggaacga atgcgatcga
     3241 actgctgccg cctaggacgg acgacttacc cgatagtcct catttcccat tgccgccggg
     3301 ccgaatgatg cgggagatgt cgcgtctcgc cgatggttac ggtttggatg tgtgggtttg
     3361 gtatccggcg atggacaaag actactcgga cccgcgcacg gttgatctgg cgatcaagga
     3421 atgggcggcg acttttcaac ttttaccgcg actcgatgca atctttgtcc caggcggcga
     3481 tcccggccgg accgcgccga agtacttact
//
LOCUS       DATNDT010000288         5500 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3756141, whole genome shotgun sequence.
ACCESSION   DATNDT010000288 DATNDT010000000
VERSION     DATNDT010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5500
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3756141"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..430)
                     /locus_tag="VN788_09230"
     CDS             complement(<1..430)
                     /locus_tag="VN788_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXU20749.1"
                     /translation="MPNSRAWALCVIFVLALASCNRGGRKRIAVVPKGTSSIFWLAVE
                     AGAMAAGDQYKVDILWNGPPMETEYDRQIQIVDSYIAQRVDGLAVAAGDRKALDHSIN
                     QAAALGIPVTVFDSGVDTTNYMTFIATDNYAAGKMAGEEMG"
     gene            complement(420..1178)
                     /locus_tag="VN788_09235"
     CDS             complement(420..1178)
                     /locus_tag="VN788_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU20750.1"
                     /translation="MTDSEPRGRVAVITGASRGLGKAMALALAAAGAKLALVARDKDK
                     LDEVSAEARKLGAEAEPFTVDVTDEVQIRKLEHDVTERFGAVHILINNAGINIRKSAE
                     DFTLDEWNRVLTTNLTSAFLLCHSFIPHMKGHGYGRILNLTSTMAHVSIAGRTAYSAS
                     KAGLLGFTKSLALELAPEKITVVAISPGPFATELNLPLINNSELNRQFISKIPVGDWG
                     KAEDIGKLAVYLCSGGTSFMTGTDILIDGGWCAQ"
     gene            complement(1239..2234)
                     /locus_tag="VN788_09240"
     CDS             complement(1239..2234)
                     /locus_tag="VN788_09240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20751.1"
                     /translation="MNLQETAIGLDIGTSRLVTARKPGKTYQFQTQLNAFLTLPFSKL
                     TEGVLRREEVPHECQDGDLLIPGNESERFANLFRLETRRPMTRGTLNPDETSGLTVIR
                     KIIRSLLGPEEGNGRRLCFSMPGVSSGSHDSLTYHEAAIRSILTEMGYEVTNINEGLA
                     VVLAELEDSNYTGIGISCGGGMCNACLAYLSIPVFSFSVPKGGDFIDSSAASVAGEGA
                     TRVRAIKEQSFHFNGFFNDKIQQALSIYYEDMISGITNALKEYLSKAKNVPRLDRPIP
                     IVLSGGSAMPAGFRDRFEKMLVPADLPIAISEVRMATEPLHSTAKGALISALAEI"
     gene            complement(2203..3300)
                     /locus_tag="VN788_09245"
     CDS             complement(2203..3300)
                     /locus_tag="VN788_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition family protein"
                     /protein_id="HXU20752.1"
                     /translation="MQDSFIERSAALIPDEVLKLRYLRWAFGRRDAPKAPPARYSRFA
                     WAVCTVLLVSNQLVCPVTPNLALPAAPPFTSPSRSGTIWIAEQHASYFLYSNGLQVRT
                     DYQTPSTRRAYRAFSRAGLVPSVQRSIPAGIVYHTTESLLLPFEPDRNRSLLATRENL
                     LDNVRRERSYNFVIDRFGQVFRIVPADEVAYHAGHSIWADAELVYCGLNESFIGVSFE
                     ARTAEAYDLTAAQIHSGRLLTDALRNLYGIPAVDCVTHAQVSVNPANMHIGFHTDWAS
                     RFPFEKLGLTGGYDTPLAAIELFGFEYDDHFLQAIGGVPWKGLAAAEEQLTRNAEKRG
                     VSLRSYRTLLQQQYQNIRRESNESTGNGDRA"
     gene            complement(3352..5115)
                     /locus_tag="VN788_09250"
     CDS             complement(3352..5115)
                     /locus_tag="VN788_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU20753.1"
                     /translation="MRGRSGVLAGLGAMVVCSVCLDIAVPFLTQRIIDGVLHAIQTRQ
                     THSMRVLLVSAAGILVATALTRLLRSVYNYKLFQTASQAEDEIKSAAFAGFLYQDTEY
                     HAGANTGEVAGSLDRGGTAIFIVLYEVLGQNLIPSLLVFGGVFAALLARNTWIAIAVF
                     LPVPVYIAIVSRLNRRMHQFEQDVTESFEAVSKEQYDVASNAAVVKKFSRERDEAAVQ
                     RELLSRARTTQFRAERLWAIFENSQTCIATLGRVVVIALGGYLVLNGRCTIGDYVLFI
                     CLQDMVYQPASQLSVILPKLRRNLSRVERLFEILDRRREVRDVEDAPALAPLRHSVEF
                     QNVCFRYADSERWALNNISFKVPAGATVALIGRSGTGKTTLVNLLQRCFDPQRGRILV
                     DGVDIRDVAQESLRRQLAVVPQEVDLFSRSILENIAYGCPSAAQREVEEAARMAQAHG
                     FIERCESGYDAQVGQRGLKLSGGERQRLGIARAILRNPKILILDEATSHLDTESERLI
                     QHATEQMMKGRTCFVIAHRLSTVRKADLVVVFAEEGIEAVGTHDDLWKTSLTYRKLHG
                     VHSGAPIFQGEVFRPAPALVN"
BASE COUNT         1130 a         1655 c         1548 g         1167 t
ORIGIN      
        1 gccccatctc ctcacccgcc atcttaccgg cggcgtagtt gtcggtcgcg atgaaggtca
       61 tgtaattcgt cgtatcgacg ccagaatcga acaccgtgac cggaatgccg agagccgccg
      121 cctgattgat ggaatggtcg agcgctttgc ggtcccccgc ggctacggcc aaaccatcca
      181 cgcgctgggc aatataagaa tctacaatct ggatctgccg gtcgtattcg gtttccatcg
      241 gcggtccgtt ccaaagaata tcgactttgt actgatcgcc cgcggccatc gctcccgcct
      301 ccactgcgag ccaaaaaatg gaggaggtgc ccttgggtac gacggcgatt ctcttcctgc
      361 ctccacgatt gcaggatgcg agggccagca cgaaaatcac gcagagtgcc caggcgcgac
      421 tattgggcac accaaccccc gtcaatcaga atgtcggtac ccgtcatgaa cgaagtgccg
      481 cccgaacaga gatagactgc gagctttcca atatcctcgg ctttgcccca gtcccccacc
      541 ggaattttcg agatgaattg ccggttcagc tcggagttat tgattagcgg aaggttcagt
      601 tcagttgcga acggcccggg actgattgcg acgacggtaa ttttctccgg cgcgagctcc
      661 agcgcgagac tcttcgtgaa tccaagcaaa ccggctttac ttgccgagta cgcagtgcgt
      721 cccgctatag aaacatgcgc catggtcgaa gttagattca gaatgcggcc atacccgtgg
      781 cctttcatgt gcggaatgaa ggaatggcac agaaggaaag cgctggtcag attcgtggtg
      841 agaacgcggt tccactcatc cagcgtaaaa tcctcagcgc tcttgcggat gttgatgccc
      901 gcgttgttga tcagaatgtg aaccgcgccg aagcgctcgg ttacgtcatg ctccagcttg
      961 cggatctgga cctcatcggt cacatcaacc gtaaacggct cggcctcggc gcccagtttc
     1021 cgcgcctcgg ccgatacctc gtccaacttg tctttgtctc gcgccaccag cgcgagtttg
     1081 gctccggccg cggccaaggc cagcgccatg gctttcccta gtccgcggct cgcacctgtg
     1141 atgaccgcaa ctcttccgcg cggttctgaa tccgtcaaag tttccacctc caagttggct
     1201 attgtaccgg ctcgggtgga acgaaacgct ttaggccgtt agatctccgc cagcgccgag
     1261 atgagcgcac ccttcgcggt cgagtgtagc ggctcggtcg ccatccgtac ttcggatatc
     1321 gcgataggca gatccgccgg gaccagcatc ttttcgaaac ggtcgcgaaa gcccgccggc
     1381 atcgcgcttc cgccgctgag gacgatcggg atgggacggt ccaggcgcgg cacattcttc
     1441 gctttcgaca gatactcttt caatgcgttc gtgatacccg agatcatgtc ttcgtaatag
     1501 atgctgagcg cctgctgtat tttgtcgttg aaaaagccat tgaaatggaa cgactgttcc
     1561 ttgatcgccc gcactctggt ggcgccttcg ccggctaccg aagcggcgct tgaatcgatg
     1621 aagtcgccgc ctttcggcac gctgaagctg aataccggta tcgaaagata cgccaggcag
     1681 gcgttgcaca tgcccccgcc gcaactaatt ccgatgcccg tataattgct atcttcgagt
     1741 tccgccagca cgacagccag cccttcattg atgttggtga cctcataacc catctccgtc
     1801 agaatgctcc ggatggccgc ctcgtgatac gtgagactgt cgtgggatcc agaggacacg
     1861 ccgggcatgc tgaaacataa ccggcgccca ttgccttctt ccggccccag aagcgatctg
     1921 atgatcttcc ggattacggt caatccactg gtttcgtctg gattgagcgt tccacgcgtc
     1981 atcggcctgc gtgtttcgag gcgaaaaagg ttggcaaagc gttcggattc gtttcctggg
     2041 atcaacaagt cgccgtcctg gcactcgtga ggaacctcct ctcttctgag tacgccttcc
     2101 gtcaacttgg aaaatggaag cgtcaagaat gcgttgagtt gagtttggaa ttgataggtt
     2161 ttgccgggct ttcgcgcggt aacaagcctg ctggtcccga tatcaagccc gatcgccgtt
     2221 tcctgtagat tcatttgatt ctctcctgat gttttgatat tgctgctgta gaagggtgcg
     2281 atagctcctc agacttacgc cgcgcttttc ggcgttgcga gtgagttgtt cttcggcggc
     2341 ggccaagcct ttccacggaa cgccgccgat ggcctgtaga aaatgatcgt cgtactcgaa
     2401 cccgaacagt tcgattgcgg caagcggagt atcgtagccg ccggtcaagc ccaatttctc
     2461 gaatggaaac cggctggccc aatccgtgtg gaagccgata tgcatgttcg ccgggttcac
     2521 ggatacctgg gcgtgggtga cgcaatctac cgcgggaatg ccgtataggt tccggagcgc
     2581 gtcggtcaac aggcggccgg agtggatttg ggcggccgtc agatcgtacg cttcggcggt
     2641 ccgcgcttca aacgacacac caatgaagct ttcgttcagt ccgcagtaga ccaattccgc
     2701 atcggcccag atggaatgcc cggcgtgata agcaacctca tctgccggta cgatacggaa
     2761 cacttgccca aaccggtcga tcacgaagtt atacgagcgc tctcgccgga cgttgtccag
     2821 aaggttctcc ctcgtagcca gaagcgatcg gttgcggtcc ggctcaaatg gcagcagcaa
     2881 gctctcagtc gtgtgataga cgatgccggc cggaatagat ctttggacgg agggaaccag
     2941 cccggcgcgc gaaaaggcgc gatatgctcg ccgagtggac ggggtctgat aatcggttcg
     3001 aacctgaaga ccgttgctgt aaaggaagta gctggcgtgt tgctcggcga tccagattgt
     3061 gccggaccgg ctcgggctcg tgaatggcgg cgcggccggc aaggccagat tcggcgtgac
     3121 cgggcaaacg agttgattcg agaccagcag gaccgtgcac accgcccaag cgaatcgcga
     3181 atagcgcgcc ggcggagcct ttggcgcgtc acgcctgcca aacgcccagc gcaggtacct
     3241 cagcttgagc acctcgtcgg gaatcagagc cgcacttctc tcaataaaag aatcttgcac
     3301 cggcttgtgt aagatggtgc tgaacctccg ggggtcctct ccggaaaacg ctcagttcac
     3361 caaggctggg gccggccgga acacttcacc ttggaagatt ggcgcgcctg aatggactcc
     3421 atgaagcttt cgatacgtca agctggtctt ccagagatcg tcgtgcgtgc ctaccgcctc
     3481 aatcccttcc tccgcaaaca cgacgaccag gtccgccttt cgcaccgtcg atagccgatg
     3541 cgcgattaca aagcaagtgc ggcccttcat catctgttcg gtggcatgtt ggatcagccg
     3601 ttcgctttcc gtgtccaggt ggctggtagc ctcatcgaga atcaggattt tcggattccg
     3661 gaggatcgcg cgggctatgc ccaggcgctg gcgttcgcca ccagagagct tgagcccgcg
     3721 ttgccccacc tgcgcgtcat atccgctttc gcagcgttcg atgaacccgt gagcctgggc
     3781 cattcgcgcc gcttcctcca cctcccgttg cgccgcgctc ggacagccat aagcgatatt
     3841 ttcgaggatg ctccgcgaga agagatccac ttcctgggga accaccgcaa gctgacggcg
     3901 aaggctttcc tgcgcgacat cgcgaatatc caccccatcc accagaattc tgccgcgctg
     3961 cggatcaaag catctctgca gcagattgac taaagtcgtc ttccccgtgc cgctgcgtcc
     4021 tattagcgcg acggtcgcgc ccgctggtac tttgaagctg atattgttca gagcccaccg
     4081 ctcgctgtcc gcgtaccgga aacagacgtt ctggaattct actgaatgcc gcagcggtgc
     4141 taatgccggc gcgtcctcca cgtcgcggac ttcccggcgc cgatccagaa tctcaaacag
     4201 gcgctcgact ctggagagat tcctccgcag tttcggcagg atcaccgaga gttgcgacgc
     4261 cggctggtac accatgtctt gcaggcaaat aaaaagtacg taatcgccaa tggtgcagcg
     4321 cccgttcaac acgagatatc caccgagcgc tatcacgacg acgcgcccca atgtcgcgat
     4381 gcaagtttgt gagttttcga atatcgccca gagccgctcg gcgcggaact gcgttgtccg
     4441 cgcgcggctc aacagttcgc gctggactgc ggcttcgtct cgctcgcgcg agaatttttt
     4501 caccaccgcc gcattgctcg cgacgtcgta ctgctccttg ctgacggctt cgaaggactc
     4561 cgtaacgtcc tgctcaaact gatgcatccg gcggttgagg cggctgacga tcgcaatgta
     4621 cacgggaacg ggcaggaata cagcaatggc gatccatgta ttcctcgcca gcagagccgc
     4681 aaatacgcct ccgaacacaa ggagagatgg aatgagattc tgcccgagaa cttcgtagag
     4741 aacgataaaa atcgcggtgc caccccggtc gagcgatccg gccacttcgc ccgtgttggc
     4801 acccgcgtgg tactcggtat cctggtaaag aaagcccgca aatgccgcgc tcttgatctc
     4861 gtcctccgcc tgcgatgccg tctggaaaag cttgtaattg tacaccgagc ggagtagccg
     4921 ggtaagagcc gtagccacca ggatcccggc cgctgaaacc agcaggacac gcatcgaatg
     4981 cgtctggcgg gtttgtatcg catgcaaaac gccatcgatg atcctttgtg tgaggaacgg
     5041 gaccgcgatg tccaggcaaa ccgagcacac caccatcgcc cccagccccg cgagcacgcc
     5101 ggatcggcca cgcaacagag aaatgacccg ccatgccaga ttccgggtgc tctggccggt
     5161 cttttgtttt atcggggcgc gaaccatttt tacgtaagtg cggtatcggg ctgattctta
     5221 tcaggaaaag acgttgtaca tacacgtact tacctcacgc gaccgggctt caaagcgggc
     5281 tggaaatgtt ttttcgaggg tcgcgtcaca ctggcaggaa ggagaaatgt atgcgacgag
     5341 aattactact tagtctcgta tttacggggg ccagcctttt cattccagca cgcagccttg
     5401 tgttcgccca ggctcccggc ggcgcccagg gtaatccgtc aatgcaaacg ccccgccaga
     5461 cctccccaga catgactcgg ccgggtgctg cgacgcccca
//
LOCUS       DATNDT010000289        20608 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2893405, whole genome shotgun sequence.
ACCESSION   DATNDT010000289 DATNDT010000000
VERSION     DATNDT010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 20608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20608
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2893405"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /gene="aroF"
                     /locus_tag="VN788_09255"
     CDS             <1..627
                     /gene="aroF"
                     /locus_tag="VN788_09255"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HXU20754.1"
                     /translation="KPRSSPYSFQGLGEEGLRLMRDAADKNDLLVISEVMDPSQIQIM
                     LPYVDVLQVGARNMQNYHLLRGLGEVDKPVLLKRAMSGTLEELLLSAEYIMAGGNYKV
                     ILCERGIRTFETALRNTLDIAAVPVLKGLSHLPVVVDPSHGTGKRDKVAPMARAAVAA
                     GADGLLIEVHPDPERALSDGVQSLYPDQFAQLMKEIRAIAPAVGRQIA"
     gene            complement(631..1422)
                     /gene="modA"
                     /locus_tag="VN788_09260"
     CDS             complement(631..1422)
                     /gene="modA"
                     /locus_tag="VN788_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXU20755.1"
                     /translation="MRSRVSSRILYVVTTLIFFCGCQFTGQPAPQTRNLTISAAISMK
                     GALDEVEHDYAAANPGVAVASNYGASGTLQLQIEQGAPVDIYISASPRQMDDLQAKGL
                     LLEGTRRNLLRNKIVLIAPKESLSHISSFQDLARSDVKPIALGDPATAPAGEYAKEVL
                     TYFGIYGAVRPKALLAKDVRQVLTYVETGDVEAGIVYSTDAASSSTVKVVATAPTASH
                     APAIYPVAVIRNSKNPRAARAFEDFLSGTRARAIFQKYGFAAANP"
     gene            complement(1531..1944)
                     /locus_tag="VN788_09265"
     CDS             complement(1531..1944)
                     /locus_tag="VN788_09265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU20756.1"
                     /translation="MQSPRLLTPRDAAKVLGISYPTVKQWIYKKKLRTIRTAGGHHRV
                     PESELDKFLHLQSERLPIEERRPNFRHISGRNQLAGRVTEIKIDGLIAQVGLSIGGQR
                     ITSIITADAVKEMRLKVGDKAAALIKSTEVMIVRA"
     gene            complement(2029..2619)
                     /locus_tag="VN788_09270"
     CDS             complement(2029..2619)
                     /locus_tag="VN788_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020595524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXU20757.1"
                     /translation="MKMDTFLAPIEKPNGLIMKLAYYFTRRQFGKVLTPLKVHSSRLP
                     PAFGLFYTKIGTLDKKLSLSPETVLLIREHVARINVCLFCIDIGRAFTIKASMNQAKF
                     DALEQYRTSPLFSDAERAALDYVTELTKNKSVNPDTFARMARYYSEREICEIVWVVAT
                     EHVYNMTNIGLNIHSDMLCDISKKEELHESQPLVHS"
     gene            2917..3651
                     /locus_tag="VN788_09275"
     CDS             2917..3651
                     /locus_tag="VN788_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019464124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXU20758.1"
                     /translation="MGKIAAFLYGLIAYLIFFVTFLYAIGFVGNFFVPKSIDSGTAGF
                     SVEALIIDALLLSLFAIQHSLMARPWFKRGWTKLIPHPIERSTYVLLASLCLDLLYWQ
                     WRPMTETVWSVENAPGQWILLGLFGLGWLTVLVSTMLVSHADLFGLRQVGLYLRGKPY
                     EPIGFKTPALYKTVRHPIYLGFLLAFWATPRMSYGHLLFAVATTGYILIAIQFEEHDL
                     ITYFGDAYRQYRLRTPMLLPFTKRAK"
     gene            3764..4882
                     /locus_tag="VN788_09280"
     CDS             3764..4882
                     /locus_tag="VN788_09280"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU20759.1"
                     /translation="MPPTRCTHEHPRRRTSHNDQAERFGNIPRRHLLRGMLVTAAAAL
                     VPNGLMRAQTAPGDAGRIDFHHHFASPRWLAKQQETKSQGWQPFQGWTPAKSLNAMDK
                     AGVSTALISCTAPGPQFPGMSLEETRSMTRDLNEYGAKMASDYKGRFGLLATLYPLDV
                     DNSLKEIEYALGTLKAVGFGLITSYGNKWMGDQAYQPVFDELNRRKALVFLHPTAAPC
                     CQNLIPNIPPPVVEYNTDTSRAIANILINGSADRTLDVHYVFSHAGGTAPYLVQRLGI
                     APAPKLADALAGTPAPGSRLYHLRRFYYDVAQSTNPIQIQALKLFAGMSQIVFGADFP
                     YTTIADHVEALQKCGLSADELKAIDRGNALRLVPQYRT"
     gene            complement(4993..5559)
                     /locus_tag="VN788_09285"
     CDS             complement(4993..5559)
                     /locus_tag="VN788_09285"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HXU20760.1"
                     /translation="MNRIVCLAAASLVCALSVSAQQRNVDTQKSTLTIHVGKSGIFSG
                     LGHEHEVRAPIHSGTADTGSHPAVEIRVDARELRVIDKDASDKERAEVQRTMLGPEVL
                     DSSDHPDIVFKSTSAEPAGQGRWTLRGNLTLRGQKRPVTVQVKQNADRYMGEATVKQT
                     DFGIKPPGKAGVKAKDEVRIEFEVRLAS"
     gene            complement(5610..6479)
                     /locus_tag="VN788_09290"
     CDS             complement(5610..6479)
                     /locus_tag="VN788_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20761.1"
                     /translation="MGSSASLKSILSFLPLLIALVQAPSALGQSAPNQTQGFGNNRLV
                     IFTYLQNFDCVDEPTLDLDFNGIMAQSDPNEMQTPICQPITEPTQDPTGGNIKHTAHL
                     YVFIPMFSVDGDTNPADAMPCPDGGRLGELCGPALGTKLIEFFGFLPEAWKAHVNPAI
                     TTQCPDPHPGVQPGTCTMHASSVDLSKTLFALGQAGLIHPPPPSPTAPVFVPTPNHSH
                     VVDDSRVNTVPIWWEVRPVLILHQSDWPAADGSSGITSSKAMDDAEAAGRAIEVGSNF
                     FLFFSSHMSMPGH"
     gene            complement(6776..7726)
                     /locus_tag="VN788_09295"
     CDS             complement(6776..7726)
                     /locus_tag="VN788_09295"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXU20762.1"
                     /translation="MTASATQHMQCMEVWGGSQLTSSGVELGGLDVWVYSKPFGQAQR
                     GGDVYYVSSCATGRISRLLLADVSGHGQSVASTAADLRTLMRRFVNRLDQKEFVRLLN
                     EEFTALSRDGGFATAIVTTFFAPTRRLSLCNAGHPRPLLFRALDARWSFLSQEESEKK
                     GPQNIPFGLFQIADYEQLDVELEPGDCLLSYTDALIESCDADGEMLGEEGLLRIVNLL
                     GDVPAEKLIPSLLQEIGERFPENLTADDVTLLAVRANGGSLDFSFREKLNAFGRFLKA
                     FSRSLNPRADRAPFPDANLANLGGAIIPALARRWRAPRPS"
     gene            8126..9085
                     /gene="cysD"
                     /locus_tag="VN788_09300"
     CDS             8126..9085
                     /gene="cysD"
                     /locus_tag="VN788_09300"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002684342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HXU20763.1"
                     /translation="MVFSELQLHLYKGEERQVGLTSQLDHLKMLEAESIFILREAAAE
                     FAHPVMLYSIGKDSSVMLRLAQKAFYPGKIPFPLLHIDTSYKFPEMIEFRDWYAKHVG
                     AELIVHKNQKALDDGANPYTLGTQKCCGFLKTRSLLDGLAEGGFDAAFGGARRDEEKS
                     RAKERVYSFRDALGQWDPKNQRPELWSLYNSRHHKGESIRVFPLSNWTELDVWLYIHS
                     EKIPIVPLYFAKEREAVVRGGSILLLQTGVHLLPGEKAQMVKCRMRSLGCVPCTGAIR
                     SDADTVPQIIEEMISFRRSERENRVIDHDEEGSMEIKKREGYF"
     gene            9085..10836
                     /gene="cysN"
                     /locus_tag="VN788_09305"
     CDS             9085..10836
                     /gene="cysN"
                     /locus_tag="VN788_09305"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF003478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HXU20764.1"
                     /translation="MSTVTPEPAEASSTTQAPAAQVAAREEPSTAASGLLRFSTAGSV
                     DDGKSTLIGRLLYDSKSVLEDQLASVQKSKVNRSSGPIDFSLLTDGLRAEREQGITID
                     VAYRYFSTPRRKFIIADTPGHEQYTRNMATGASTAEAAVVLMDATKGVLRQSRRHAYI
                     AHLLGVRHIVAAVNKMDLVAFSQEVFERISADFREFADQLGIKNVYAVPVSALEGDNV
                     VRRSKRMPWFEGPALLEHLESLPVGADSTSGPLRFPVQYVIRPDASFRGFAGQVVSGE
                     VRPGASIMALPSGVKTRVQSIVAFEEELESAGQGSAVTITLQDEIDISRGDMLVAEDQ
                     RPAASTEFQATLVWMHSEPLDPHKIYVLKHTTKTVRARVNQIRHRVDINTLQHTPATK
                     LELNEIGAVDVKTTLPLFFDPYKENRTTGSFILIDPLHNATVAAGIIERAIETGSRAT
                     ATHRKHSTKEERILRFGHPAAAVWLKGRPHVAELVERQLFDEGWQVQLAGPTDFLSHE
                     LVTVAKAFRLSGYITVFCPLDDGTDQKQVVRAIFGPESFFAVRITDDTDEEAVERIMK
                     VLRKWRSTYSDAHKDKL"
     gene            10833..11546
                     /locus_tag="VN788_09310"
     CDS             10833..11546
                     /locus_tag="VN788_09310"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013704645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HXU20765.1"
                     /translation="MSQTPILAEEFETLSTEAVIDRLLAENSAAAKKASICLTCSFQA
                     EDMIVLDLLRRRLPDIPVLFLDTGYHFAETYAYRDRMAKAWNLNVRNLAAKQSVVDQE
                     SQFGILNRTDPGRCCHLRKVEPLFSALEEFDVWFTGLRREQSPTRKNLKIVEHHELPS
                     GKILLKASPLAAWTWGQIWKYTAENKIDYLPLYDSGYRSIGCEPCTAIPAEGADPRSG
                     RWGGAKLECGIHTESKRTV"
     gene            11547..12452
                     /locus_tag="VN788_09315"
     CDS             11547..12452
                     /locus_tag="VN788_09315"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXU20766.1"
                     /translation="MHILIGFLIALAVGLTGIGGGSFTVPALVLIVGLSAETAVGTAF
                     VFAGVLRLIAAPFYLAGRRIHGRYLWLLSLGAVPGLLLGTYILRALSGNADSPVVIIL
                     LGLLLTSSSTITFIPRAQNRNFAKKNSRWLPWLALPIGVEAGFSSAGAGALGTVLLLN
                     YSEMTPPEVVGTDILFGLVLAVLGSAFHWRFGSINGHVLTQLLEGGIPGVIIGCASAR
                     YLPARKLKFVIAVIAIFAGLQLVWTGSRTLLSRHSRNRAPFQSSSKSARAVVRQAETS
                     RFLQESTRSLSFNKTSGHSPSIATL"
     gene            complement(12435..13097)
                     /locus_tag="VN788_09320"
     CDS             complement(12435..13097)
                     /locus_tag="VN788_09320"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HXU20767.1"
                     /translation="MTTMRPSSRITRIIGMMSFAVAASGLSLVFAGTLFCQGMNRPQQ
                     YPPQTQRPATGEPGEPGISTPAMNTMSDAEFAQQAAEGGAAEVKLGQLAQEKGSNEAV
                     KDFGKMMVNDHTEAGQQLKTIAAQQNLKLPDEPNKHDQGVYNKLSKLSGEAFDRAYAK
                     QMVNDHRKVIDDFQQEASGGQNQSIKSFAAQTLPTLQKHLKNAREMQRTVSRSGGSKS
                     GY"
     gene            complement(13179..13787)
                     /locus_tag="VN788_09325"
     CDS             complement(13179..13787)
                     /locus_tag="VN788_09325"
                     /inference="COORDINATES: protein motif:HMM:NF025013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4142 domain-containing protein"
                     /protein_id="HXU20768.1"
                     /translation="MKIETIRKVTFLVVFAGVSLFAAGLLLGQETTASSTSSKDSATN
                     TMADTHFAKEAAAGGMAEVKMGKLAQEKGSNETVKSFGEKMVQDHSEANEKLKSVADE
                     QKIKLPTTLSKQDQATYDRLSALSGDAFDKAYARDMVNDHVKDIAAFKKEATGGKNEA
                     VKNFASETLPTLQTHLKMAHEMAQAVGVSTTAKHTGSATTTH"
     gene            complement(13916..14467)
                     /gene="sufT"
                     /locus_tag="VN788_09330"
     CDS             complement(13916..14467)
                     /gene="sufT"
                     /locus_tag="VN788_09330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HXU20769.1"
                     /translation="MATEIITLSRDCDAIEIPSGTKGVLPSGTDVRVMQFLGSSWTVT
                     SPHGMFRVDVKDADALGLTPPVAPAEGAPGAPEGGFNEKMVWDQLKTVFDPEIPVNIA
                     DLGLIYSCVTAPLDQGGHRIDIKMSMTAPGCGMGNVLKADVENKLGNLPDVKEVHVEI
                     VFDPPWNPSLMSEAAKLQLGFDL"
     gene            complement(14491..14940)
                     /locus_tag="VN788_09335"
     CDS             complement(14491..14940)
                     /locus_tag="VN788_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009770556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HXU20770.1"
                     /translation="MSELRDLYQDVILEHSKAPRNYRELPAANHKAEGFNPLCGDRFT
                     IYVTTEGDSIRDITFQGSGCAISKASASMMTQSLKGKSTAEASKIFERFHKLVTGQQS
                     SNGDGELGKLTVFSGVSEFPVRVKCATLAWHTLQAALEDKQEPVSTE"
     gene            complement(14940..16241)
                     /locus_tag="VN788_09340"
     CDS             complement(14940..16241)
                     /locus_tag="VN788_09340"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002610559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HXU20771.1"
                     /translation="MSTAANKLETRPRAPARGRDGSAQTHTSGLDIEKIRADFPILHQ
                     TVHGKPLVYLDNAATSQKPRAVIDAITRYYEGTNSNIHRGVHFLSEQATAEYEAARQT
                     AQKFINAAHAHEIIFVRGTTEGINLVAQTYGRAQIRQGDEVLITAMEHHSNIVPWQIL
                     CDERGAKLRVAPINDQGELLLDEFARLLGPRTKLAAFTQVSNALGSVNPVKQMIELAH
                     GKKIPVLIDGAQAAPHIKVDVQALDCDFYTFSGHKVYGPTGIGVLFGKSALLNAMPPY
                     QGGGDMISSVTFEKTTYNKLPHKFEAGTPDIAGVIGLGAALSYVNGLGIENIAAHEHE
                     LLAYATEKVSAIPGVSLIGTAKERAGVISFVVEGVHPHDVGTILDQEGIAIRTGHHCS
                     QPVMERFGIPATARASFAVYNTFEEIDALVRGIGKVREVFA"
     gene            complement(16238..17599)
                     /gene="sufD"
                     /locus_tag="VN788_09345"
     CDS             complement(16238..17599)
                     /gene="sufD"
                     /locus_tag="VN788_09345"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HXU20772.1"
                     /translation="MATASAGLQNYEETFGGFEKSAAGRELSWLRELRQSGFARFSET
                     GFPTTRDEDWRFTNPAAIAQTHFRLVRNGHSLPSKDQLAAFRVPGAGCQAVFVDGRFA
                     PQLSTIGKLPVGVTVASLAAELARNPGAVEPYLGRYLDLQRDAFGALNTAFLEDGAFV
                     HLAKGTVAEEPIALLFVSTGHDAPTVSYPRNLIVAEENSQLTIVEDYVSLGGGEALCN
                     TVTELVAGDHSVVSHYMIEREHVKTYNVSTLRIQQGRSADVASHSVLIGGALVRNNVH
                     PVLRGEGGECLINGLFIGNGRQHLDNYMLVEHASPHCASRQFYNGILGDRAHGVFHGR
                     IIVHKDAQKTDAKQTNRNLLLSDTAQIDTKPQLEIYADDVKCTHGATIGQIEENALFY
                     LRSRGIDEASARTLLLVAFASECLDRMKEGPVRSHVERLVQEQALRMAGTSHPMEQRD
                     QKR"
     gene            complement(17633..18385)
                     /gene="sufC"
                     /locus_tag="VN788_09350"
     CDS             complement(17633..18385)
                     /gene="sufC"
                     /locus_tag="VN788_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008020337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HXU20773.1"
                     /translation="MLEIKDLKVRVDAREILKGINLKLNAGEVHSIMGPNGSGKSTLA
                     QVLARRETYEVTGGEVLFEGKDLLAMKPEEAACEGLFLAFQYPVEIPGISNAYFLRSA
                     LNAIRKYRGQEEMDAMDFLPKLREKLKLLDMDEKLLNRSVNEAFSGGEKKRNEILQMA
                     VLEPKLAILDETDSGLDIDALRIVAQGVNAMRNPGRTMLVVTHYQRLLNYIVPDYVHV
                     LVDGRIVKSGGPELALDLEDKGYGAFEAAAGA"
     gene            complement(18521..19978)
                     /gene="sufB"
                     /locus_tag="VN788_09355"
     CDS             complement(18521..19978)
                     /gene="sufB"
                     /locus_tag="VN788_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002810842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HXU20774.1"
                     /translation="MSSNTDTIRELAQQEYKWGFVTDIGEDRAPKGLNEDIIRLISTK
                     KNEPEFMLEWRLKAYKHWDSLEKAQAEPKWANVHYPPIDYQAISYYSAPKQKANLKSL
                     DELDPEILRTYEKLGIPLAEQKMLAGVAVDAVFDSVSVATTFKSKLAELGIIFCSFSE
                     AVQNHAELVKKYLGSVVPYTDNFFASLNAAVFTDGSFVYVPKGVRCPMELSTYFRINA
                     AETGQFERTLIIADEGASVSYLEGCTAPIRDENQLHAAVVELIALDNAQIKYSTVQNW
                     YPGDKNGKGGIYNFVTKRGACRGKNSKISWTQVETGSAITWKYPSCILQGENSVGEFY
                     SVALTNNYQQADTGTKMIHIGKNTRSTVVSKGISAGHGQNTYRGQVTIMKGAAGARNY
                     TQCDSLLIGDKCGAHTFPYIEVKNSTARMEHEASTSKIGEDQMFYLRQRGLKAEDAVS
                     MIVNGFCKRVFRELPMEFAVEAQKLLSVSLEGSVG"
     gene            complement(19975..20451)
                     /locus_tag="VN788_09360"
     CDS             complement(19975..20451)
                     /locus_tag="VN788_09360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HXU20775.1"
                     /translation="MVRLGKLTDYGLVLMTCIARTQGAPLRTARDLARESKLPISTVS
                     KLLKELLQSGLLISHRGTKGGYVLAREPRNISVLEMIAAIEGPMALTECSTDITGLCN
                     LESYCPIKSNQQIINRAVRSVLEKVTLSDLMHPLQLTTIEDARGKVLPVIASGRVQ"
BASE COUNT         4190 a         6076 c         5702 g         4640 t
ORIGIN      
        1 aagccgcgct cgtcacccta tagcttccag ggattgggcg aggaaggcct gaggctaatg
       61 cgcgacgcgg cggacaagaa cgatttgctg gtcataagcg aggtgatgga cccatcacaa
      121 atccaaatca tgctgccgta tgtggacgtg ctccaggtag gcgcgcgcaa catgcagaat
      181 tatcacctgc tgcgtggtct tggcgaagtc gataagccgg tcctgctgaa gcgcgcgatg
      241 tctggaacgc tcgaagaact gttgctgagc gctgagtaca tcatggccgg tgggaactac
      301 aaagtgatcc tctgcgagcg cggcattcga accttcgaga cggccttgcg caacacgctc
      361 gatatcgcgg cggtgccggt gctgaaggga ctctcacatc ttccggtcgt ggtggaccct
      421 tcgcacggaa cagggaagcg cgacaaagtc gcgccgatgg ctcgcgcggc cgtggcagcg
      481 ggcgccgatg gattgctgat cgaggtacat cccgatccgg agcgagcgct ttctgacgga
      541 gtacaatcgc tctacccgga tcagttcgcg caattaatga aggaaatccg cgcgattgcg
      601 ccggcggtcg gaaggcaaat cgcgtgattg ctacgggttc gcagcggcga atccgtactt
      661 ctgaaagatg gccctcgccc gagtacctga gagaaagtct tcgaatgcgc gagccgcccg
      721 gggattctta gagtttctga tcacagcaac cgggtagatc gccggtgcat gcgacgctgt
      781 aggagccgtg gcgacgactt tgaccgtcga ggacgaggca gcatcggtgg aatatacgat
      841 acctgcttcc acgtctcccg tctcgacgta ggtcaacacc tgccgcacgt ccttggcgag
      901 cagagctttg ggccttaccg cgccataaat tccaaaataa gtgagcacct ctttcgcgta
      961 ctcccccgct ggagcggtcg cagggtcgcc cagcgcgatt ggcttcacgt cggaccgcgc
     1021 caaatcttga aacgaagaga tgtgcgataa cgattccttc ggggcaatta acacgatctt
     1081 atttcgaagc aaattcctgc gcgttccttc gagcaaaagc cctttggcct gaagatcatc
     1141 catctgcctc ggagaagcgg aaatataaat gtccaccggc gctccctgtt cgatctgaag
     1201 ttgaagagta ccggaagcgc cataattgct tgcaacggcg acgcctggat ttgcggccgc
     1261 atagtcatgc tccacttcgt ccagggcgcc tttcatgctg attgccgccg aaatggtcag
     1321 atttctggtt tgcggagctg gctggcctgt aaattggcac ccgcaaaaga aaataagcgt
     1381 cgtaacgacg tacaggatgc ggctggatac tcggctgcgc acaggtcgat ttaacgcggt
     1441 tcaacacgga gtgtcaatat ggtgctacgc gattggggtt tgtagcgacg ggccggggag
     1501 gattcgaaaa gcgcagacct tgcgaaaagc ctatgcgcgg acgatcatta cctccgtgga
     1561 tttgatcagc gctgcggctt tatctcccac cttcagccgc atttccttca ccgcatccgc
     1621 ggtgattatc gaggtaattc tctggccgcc aatcgaaagc cccacctgcg cgatcagccc
     1681 gtcgattttg atctctgtca cgcgaccggc gagttgattg cggccgctta tatggcgaaa
     1741 gttcggccga cgttcttcaa tgggcaggcg ctccgattgc aggtgcagga atttatccaa
     1801 ctcggactcc ggaacgcggt gatggcctcc ggcagtccta atcgtgcgca actttttctt
     1861 ataaatccat tgcttcacgg tcggatagct gatgcccaaa accttagcgg catcgcgggg
     1921 agtcaagagt ctgggagatt gcatggcgtt tcggcgacag ccccatgttt ctagcctact
     1981 tgggctgctg cgtcaactgg cgccgcccac tcgcccgaaa aatcgccgct atgagtggac
     2041 aagaggttga ctctcgtgca attcctcctt tttgctgatg tcgcagagca tgtccgaatg
     2101 gatgttcagg ccgatgttgg tcatgttgta gacgtgctcg gtggcaacca cccagacgat
     2161 ctcgcagatc tcgcgctcgg aatagtagcg cgccatgcga gcaaaagtgt ccgggttcac
     2221 acttttgttc ttcgtcagct ccgtcacata atcgagtgcg gcgcgctccg catcgctgaa
     2281 aagagggctg gtgcggtatt gctccaaagc atcgaattta gcttgattca tcgaggcttt
     2341 gatcgtgaat gcccggccaa tatcaataca aaagaggcag acgttgatac gcgccacatg
     2401 ttcacggatg agcagaaccg tctccggcga cagcgagagc tttttatcga gcgtgccaat
     2461 cttggtataa aacagaccga atgcaggtgg tagccgcgag gaatgcacct tcagcggtgt
     2521 cagtactttg ccaaattgcc gccgcgtaaa gtaatacgcc agcttcataa tcaatccatt
     2581 tggcttttcg atcggagcaa gaaatgtatc cattttcatg ccctcccttc tccccatatt
     2641 cgattttcgc tcgaacccgg tcgatgcatc ttttttcgag cgtgatgcac ggttctccca
     2701 tacagagcgt cgaagagtcg aatcggcgtc ggcggcgttg ttcccaacgg gcccactttt
     2761 ttgggctgca accttcttga ctgcggaggc acggcgggaa taccatgcgg gccgatcgtt
     2821 gattcctata cccgagaagc atcgcggtca ttatctaggc tacacagcct atgaaatcct
     2881 tggaggtaaa tgtcgcctta gctcaggagg cacaccatgg gcaagatagc agcatttctg
     2941 tacgggctca tcgcatatct gattttcttc gttacgtttc tctacgcgat cggttttgtc
     3001 ggcaacttct tcgttccgaa atccatcgat tccggtacgg cgggtttttc cgttgaggct
     3061 ctgataatcg atgctctctt gctgagcctc ttcgccatcc agcatagcct gatggcgcgt
     3121 ccctggttca agcgcgggtg gacgaagctt atccctcacc ccattgaacg aagcacatat
     3181 gtcctgctcg ccagcctatg tctcgacctg ctctactggc aatggcggcc aatgacggaa
     3241 accgtgtgga gcgtcgagaa cgcccctggg cagtggatac tcctcggact tttcggtctt
     3301 ggctggctca ccgttttggt cagcacgatg ctggtcagcc acgccgatct ctttgggctt
     3361 cgtcaggtag gtctgtactt gcgcggcaag ccatatgagc cgattggttt caagacgccc
     3421 gcgctctaca agaccgtccg tcatcccatc tacctgggat tccttctggc cttctgggcg
     3481 acgcccagga tgagttacgg ccacttgctc ttcgccgtgg cgacaacagg ctacatcctc
     3541 atagctattc aattcgagga acacgacctg attacttact ttggggacgc ctacaggcag
     3601 taccggctgc ggaccccaat gcttcttcca ttcacgaagc gcgcaaaata gttgcggccg
     3661 ctgctctccg cggagcacgc tgatcagccc ataatcactg tgcctgaatt gcaccccgcg
     3721 agcggttgac acgtgcctac actccacgga acaattgcgc cttatgccac cgacccgttg
     3781 cacccacgaa caccctcgcc gccgcacttc acataacgat caagccgaac gatttggcaa
     3841 catcccgcgc cgccatctgc ttcgcggcat gctggtgact gccgctgctg ctttggttcc
     3901 aaatgggttg atgcgcgctc aaaccgctcc cggcgatgcc ggccgcatcg atttccatca
     3961 tcattttgca tcacctcggt ggctcgccaa gcagcaggaa accaagagcc aaggctggca
     4021 gccattccag ggctggactc cggccaagtc cctgaacgcg atggacaaag ccggcgtatc
     4081 gaccgcgctg atctcttgca ccgcgcccgg cccacaattt cccggcatga gtctcgagga
     4141 gactcgcagc atgacgcgcg atctaaacga atacggcgcg aagatggcct ccgactataa
     4201 gggccgcttc ggcctgctcg ccaccttgta tccgctggat gttgataaca gcttgaaaga
     4261 aatcgagtat gccctcggca cgttgaaagc ggtcggcttc ggactaatta ccagctatgg
     4321 caataaatgg atgggcgacc aggcatacca gccggtcttt gatgaattga accggcgcaa
     4381 ggcgctcgta ttccttcatc ccacggcggc gccctgctgc cagaacctga tccccaatat
     4441 tcctccgccc gtggtggaat acaataccga taccagccgc gccatcgcga atatcctcat
     4501 caacggctcc gccgaccgca cgttggatgt tcattatgtg ttttcgcatg ccggtggcac
     4561 cgcgccctat ttggtgcaac gtctcggcat cgccccggcc cctaagctcg ccgatgcgct
     4621 ggccggaacg cctgcgccgg gctcccgctt gtatcatctc cgccgctttt attacgacgt
     4681 ggctcaatcg acaaacccga ttcaaataca ggctctgaaa ctgttcgcgg gaatgtcgca
     4741 aattgttttt ggcgccgatt ttccctacac cacgatcgcg gaccacgtcg aggcgctcca
     4801 aaaatgtgga ttgagcgcgg acgagctcaa agccattgat cgaggcaatg cgttgcgtct
     4861 ggtaccgcaa tatcgcacct gaggaaatta ggcaacccgc aagcgatttt ctgagcgaat
     4921 ataatcgcca gtaaagacca gtttcgctac gccggtaatg aggcactctg tcgtactcat
     4981 tcgccgcgcg tcttacgacg ctaatcgcac ttcaaactca atcctaactt cgtctttggc
     5041 tttgacgccc gcctttccag gtggcttgat cccgaagtct gtctgcttca ctgtggcctc
     5101 gcccatgtaa cgatcggcgt tctgcttcac ctgcaccgtc acaggccgct tctgaccgcg
     5161 caatgtcaga ttcccgcgca aagtccagcg cccttggccg gcaggctccg cacttgtgga
     5221 tttgaatacg atatccggat gatcggagct atcgagcact tcgggtccta acatcgtcct
     5281 ctgcacttcg gcgcgctcct tgtcggaagc gtccttgtcg atcacgcgca gctcccgcgc
     5341 atccacccgt atttcaaccg ccggatgcga gccggtatcg gcggtaccgc tgtggatcgg
     5401 ggcgcgcact tcgtgctcat gtccaagccc tgagaagatg cctgacttgc cgacatggat
     5461 tgttaacgtg gacttttgcg tgtcgacgtt gcgctgttgg gcgctcacgc tcagcgcgca
     5521 caccaagctc gctgcagcca gacacacgat tcgattcatc gctctcctca aacagagttt
     5581 ccggaatgaa cgcgaactac aattccagtc taatggcccg gcatggacat atgggagctg
     5641 aagaacagga agaagtttga accaacttcg attgccctgc cggccgcttc cgcatcgtcc
     5701 atcgctttcg aggaggtgat gccgctgctc ccatcggctg caggccagtc gctttggtgc
     5761 agtatcagca ccggtctgac ttcccaccag attggcacag tgttcacccg gctgtcgtca
     5821 accacgtgac tgtgattcgg ggttggcacg aaaacggggg ccgtcggtga tggcgggggc
     5881 ggatggataa ggcccgcctg gccgagtgca aacaaagtct tggaaagatc gactgatgag
     5941 gcatgcatcg tgcacgtgcc cggctgcacg cctgggtgtg gatcggggca ttgggtcgtg
     6001 atggctggat tcacatgtgc cttccaggcc tcgggaagaa agccgaagaa ttcgatgagt
     6061 ttggtgccca gtgcaggccc acacagctca cctagccgtc ctccgtcggg gcagggcatg
     6121 gcgtcagcgg ggttcgtgtc gccatcaacc gagaacattg gaatgaacac atagagatgc
     6181 gcggtgtgct tgatgtttcc acccgtcggg tcctgtgtgg gctcggtgat gggctggcaa
     6241 atgggggtct gcatctcatt ggggtcggac tgcgccatga ttccattgaa atcgaggtca
     6301 agcgtgggct catcaacgca gtcgaaattc tgcaggtacg tgaatatgac tagtctgttg
     6361 ttgccaaaac cctgcgtctg attgggggct gactgcccga gtgcacttgg tgcctgaaca
     6421 agagcgatca gcaatggcaa aaatgagagg atcgatttaa gtgacgccga gctgcccata
     6481 ggagtcctca cttcctttgg ctggggatca cagcttgtct cctggatagc cgacgcttgt
     6541 cacagctagg tctctggcct gtaattttct tgtaattact tgtgagcctt gcgaactgca
     6601 cgtggggtta cgggcttacc ggaaggatgg ccttgcagga aaagaaagcg cgtgtaaagg
     6661 aaaggagccg atccgaaacg gcgaatcggc tcttcggcga atttcttttg aacagacata
     6721 cccttatcgc gggtttgagc taagtccgag tgagcaagag ttggcaagtt atttctcagg
     6781 atgggcgagg ggcacgccag cgacgagcga gcgcggggat aatggcgccg ccgagattag
     6841 cgagattagc atcgggaaac ggggcccgat ctgcgcgggg gttcagggag cgggagaagg
     6901 ctttcaggaa gcgcccgaag gcattaagtt tttcgcggaa actgaagtcg agtgaacctc
     6961 cattagcccg aacggcgagg agggtcacgt cgtcggcagt gaggttttca gggaagcgct
     7021 caccgatttc ctggaggagc gatgggatga gtttctccgc gggaacatcg ccgagcagat
     7081 tgacgatacg aagcaacccc tcttcgccga gcatttcgcc gtctgcgtca caggattcga
     7141 tgagcgcgtc ggtgtagctg aggaggcagt ctcccggttc gagttcgaca tcaagctgct
     7201 cgtaatcggc gatctgaaag agtccgaagg gaatgttctg cggccctttc ttttcggact
     7261 cctcctggct gagaaaggac caacgggcat cgagagcgcg aaagagaagc gggcggggat
     7321 ggccggcatt acaaagtgag aggcggcgag tcggcgcgaa gaacgtagtg acgatggcag
     7381 tggcgaaacc gccatctcgg gagagcgccg tgaactcttc gttgagcagc cgaacaaatt
     7441 ctttctggtc gaggcgattg acaaaacgac gcatcagtgt gcgcaggtcg gctgccgtcg
     7501 aggcgacaga ttgtccgtgg ccggaaacgt cggcaaggag caggcggctg atgcggcctg
     7561 tcgcgcagct cgaaacgtaa tagacatcgc cgccgcgctg ggcttggccg aagggcttgc
     7621 tatagaccca tacatcaagg ccacccagtt cgacaccgct ggaggtgagt tggctgccgc
     7681 cccaaacctc catgcattgc atgtgctgcg tggccgaagc cgtcatggga ggcattatag
     7741 cggctgagag gcaactctcg atgcagctct gagggctctc agtatcgcta tgtcagcgcg
     7801 gatcgttccg gcgctgcctg ctgctcgcgt tcgtaagcgg aatggaggag atctcgctct
     7861 atggagctgc tgagtcacgc ttgaggtcga tcctaagtcc ttgtatcgac ttaccggaac
     7921 cacgccgaca tttgacagca agggcttttt atactagact gatttagtac tttatgagct
     7981 tagcgacgaa gaagacctgt tgcgcttgtt gttgtagcac cgcaatgtgc gtgtgccggg
     8041 gcgctgggtt tctgagctaa gttcgcaaca caacaaggct taagtttcac ccgccctcgg
     8101 gaattaccga gaggcgggtt tttttatggt ctttagcgag cttcagttgc atctgtacaa
     8161 gggagaagaa agacaagtgg ggttaacgag ccaactcgac catctgaaaa tgctcgaagc
     8221 cgagagcatt ttcatcctgc gcgaagctgc cgccgaattt gcgcatccgg tgatgctcta
     8281 ttcgatcggc aaggattctt cagtaatgct gcgcctggca cagaaggcat tctatccggg
     8341 gaagatccca ttcccgcttc tgcacatcga taccagctac aaattcccgg aaatgatcga
     8401 atttcgcgat tggtacgcca aacacgtagg cgccgaacta attgtacaca agaaccagaa
     8461 ggctctcgac gatggcgcaa atccctacac tttgggcacg caaaaatgct gcggctttct
     8521 caagacacgt tcgcttttgg acggactcgc cgaaggcggg ttcgacgcag ccttcggagg
     8581 cgcgcgtcgc gacgaggaaa aatcgcgggc caaggagcgc gtgtattcat ttcgcgatgc
     8641 gctcggccag tgggacccga aaaaccagcg tcccgagctt tggagccttt acaacagccg
     8701 gcaccacaag ggcgaaagca tccgcgtttt cccgctttcg aactggaccg aacttgacgt
     8761 ctggctgtac atccactcgg aaaaaattcc catcgttccg ctgtattttg ccaaggaacg
     8821 ggaagcggtt gtgcgcggcg gatcgattct gctcctgcag accggcgtac atctgctgcc
     8881 cggagagaaa gcgcagatgg tgaagtgccg gatgcgttcg ctgggatgcg tgccatgcac
     8941 cggcgcgatc cgttcggatg cggataccgt tccccaaatt atcgaagaga tgatttcgtt
     9001 ccgccgctcc gagcgcgaaa atcgcgtgat cgatcatgac gaagaaggct cgatggagat
     9061 caaaaagcgc gagggatatt tttagtgtcc accgttactc cagaacccgc tgaggcgtcg
     9121 tccaccactc aggcgccggc ggcgcaagtg gccgcgcgcg aggaaccctc gacagccgcc
     9181 tccggactac tgcgattttc cacagcaggc agcgtggacg atggcaaatc cactttgatc
     9241 ggccgcttgc tgtacgacag caaatccgtt ctcgaagatc agctcgcgtc cgttcagaaa
     9301 agtaaggtca accgttcgag cggcccgatt gatttttcgc tactcacgga cggtctgcgc
     9361 gccgagcgcg agcagggaat caccatcgac gtcgcctacc gatatttttc cacgccgcgc
     9421 cgcaaattca tcattgccga cactcccggc cacgagcagt acacgcgcaa tatggcaacc
     9481 ggcgcttcca ccgctgaagc cgccgtggtc ctgatggatg caaccaaggg agttttgcga
     9541 cagtcgcgcc gccacgcata catcgcccat ctgctcggag ttcggcacat cgttgccgcc
     9601 gtaaacaaaa tggatttggt ggcgttttcg caggaagtat tcgagcgcat ctccgccgac
     9661 ttccgcgagt ttgccgacca gttgggaata aaaaatgtct acgcggtgcc agtaagcgcg
     9721 ctcgaaggcg acaacgtggt gcgccgcagc aagcgtatgc cctggtttga gggccctgcg
     9781 ctccttgaac atctcgaatc gctgcccgtc ggcgcggact caacttcagg gccgttgcga
     9841 tttcccgtgc aatacgtcat tcgtcccgac gcgagttttc gcggcttcgc aggccaggtc
     9901 gtttccggag aagtccggcc cggtgcaagc atcatggcgc tgccctccgg cgtgaagacg
     9961 cgcgtgcaat ccatcgtagc gttcgaagag gaactcgaat ccgccgggca gggaagcgcg
    10021 gtcacaatta cgctgcagga cgaaattgac atcagtcgcg gcgacatgct cgtcgcagaa
    10081 gatcagcggc ccgccgccag cacggaattt caagccacgc tggtgtggat gcactccgag
    10141 ccgctcgacc cgcacaaaat ttacgtgctg aagcacacga ccaaaaccgt ccgcgcccgc
    10201 gtgaaccaaa tccgccaccg cgtggatatc aatacactcc agcacacacc cgcaacgaaa
    10261 ctagagctga atgaaatagg ggcggtggat gtgaagacca ctctgccgct tttcttcgat
    10321 ccctataaag aaaaccgcac cacgggcagc ttcattttga ttgacccgct gcacaacgcc
    10381 acggtcgccg cgggaatcat cgagcgcgca atcgaaaccg gatcgcgagc gacggccacg
    10441 caccggaagc acagcaccaa ggaagaacgc atcctgcgct tcggccatcc cgcagctgcg
    10501 gtctggctaa aaggccggcc gcacgtcgct gaattggtag agcgccagct attcgacgaa
    10561 ggctggcagg tgcagctcgc gggccccact gattttcttt cgcacgaact tgtcaccgta
    10621 gccaaggcgt tccgcctctc cggctacatc acggtatttt gtccactcga cgacggcacc
    10681 gatcaaaagc aagtagttcg cgcaattttc ggtcctgaat cgtttttcgc cgtgcgcatc
    10741 accgacgaca ccgacgaaga agccgtcgag cgcatcatga aagttctgcg aaaatggcgt
    10801 tccacgtatt ccgatgcgca caaggacaag ctatgagcca gacgcccatc ctcgccgaag
    10861 aatttgagac gctctccacc gaagccgtca tcgaccgtct gctagccgaa aatagcgccg
    10921 cggcaaaaaa agcatcgatc tgcctaactt gcagcttcca ggccgaagac atgatcgtcc
    10981 tcgatctgct acgccggcgc ttgccggata ttccggtgct gtttctcgac accggctatc
    11041 acttcgcgga aacctacgcg tatcgcgacc gcatggccaa agcctggaat ttgaacgtgc
    11101 gaaatctcgc agcgaagcag tctgtggtcg atcaggaatc tcaattcggc atcttgaatc
    11161 gcaccgatcc cggtcggtgt tgccatctgc gaaaagtcga accccttttc agcgcgctcg
    11221 aggaattcga cgtgtggttc acgggcctgc gtcgcgagca atcgcccacg cgcaaaaatc
    11281 taaaaattgt cgaacaccac gagctgccgt cgggcaaaat cctcctgaaa gcgagccccc
    11341 tcgccgcttg gacctggggc caaatttgga aatacaccgc ggaaaataaa atcgattacc
    11401 tgccacttta cgattccgga tatcgaagca tcggctgcga gccctgcacc gcgattccgg
    11461 ccgaaggcgc tgatccgcgg tcgggccgct ggggcggagc caagcttgaa tgcgggatcc
    11521 acaccgagtc caagcgaacc gtctaaatgc acatcctgat cggcttcctg atcgcgctcg
    11581 cggtcggcct cacgggcatt ggtggcggca gtttcacggt gccagcgctg gttctaatcg
    11641 tcggcctctc cgcggaaacc gcggtgggta ccgctttcgt ttttgcgggc gtgctgcgtc
    11701 tgatcgccgc gccgttctat ctcgcgggac gccgaatcca cggccgctac ctgtggctgc
    11761 tgtctctagg cgcggttccc gggcttctgc tgggaactta catcctgcgc gcgctcagcg
    11821 gtaacgccga tagccccgtc gtcataattc ttctcggcct tctgctcacc tcgtcttcca
    11881 ccatcacttt cattccccgc gcccaaaatc ggaatttcgc gaaaaagaac tcgcgctggc
    11941 tgccttggct ggcgttgccg atcggggtcg aagccggatt ttcctcggcc ggcgcgggcg
    12001 cgttgggcac cgtgctcctg ctgaattatt ccgaaatgac tccgccggaa gttgtaggca
    12061 ccgatatttt gttcggactc gtcctggccg tgctcggcag cgcattccac tggaggtttg
    12121 gctccatcaa cggtcacgtc ctcactcagc tgctcgaggg cggaatcccg ggcgttatca
    12181 tcggatgcgc atcggcgcgc tatctccctg cgcgaaaatt aaagttcgtg atcgcggtga
    12241 ttgcaatttt tgcgggatta caactggtgt ggaccggttc gagaacgctg ctgtcacggc
    12301 actcacgaaa ccgtgcgccg tttcagtcca gcagcaagag cgcccgcgct gtcgttcggc
    12361 aggccgaaac cagccgtttc cttcaagaat caacaagaag tttaagcttc aataagactt
    12421 ctgggcactc tccatcaata gccactcttt gacccaccgg atcgcgaaac tgtccgttgc
    12481 atctcgcgtg cattcttcag gtgcttttgc agcgtgggta aagtctgcgc ggcgaaactc
    12541 ttgatggact ggttttgtcc tccacttgcc tcttgctgga aatcgtctat aactttgcga
    12601 tggtcgttta ccatttgttt cgcataagcg cggtcaaagg cctcacccga aagtttggaa
    12661 agcttgttat agactccctg atcgtgcttg ttcggttcgt ccggcagctt caggttctgt
    12721 tgagccgcga tggttttcaa ttgctgccca gcctcggtgt ggtcattcac catcattttg
    12781 ccgaaatctt tcacggcctc gtttgaacct ttttcctggg cgagttggcc cagctttacc
    12841 tctgccgccc cgccttcggc ggcctgctgg gcaaattccg cgtcgctcat ggtattcatg
    12901 gctggcgttg atatgcccgg ttccccgggc tccccggtgg ctggcctttg ggtctgcgga
    12961 gggtattgct gcggacgatt catgccttgg cagaacagcg ttcccgcgaa aaccagcgac
    13021 aagccgctag ccgcaactgc aaaactcatc attccaatga tccgtgtgat gcgcgagctt
    13081 ggcctcatgg tagtcatatt tacctccgcc gccctgatgc ctgagattgc gggtgcactt
    13141 caccccgcgt caagacgggc ttagttgaaa ggcatactct agtgagtggt cgttgcactg
    13201 cccgtgtgtt tcgcggtcgt cgaaactcca actgcttgcg ccatctcatg cgccatcttc
    13261 aagtgggttt ggagcgtggg cagagtctcc gaagcaaaat ttttcacggc ctcgtttttg
    13321 ccgccggtag cttctttctt aaaggctgca atgtctttca cgtggtcgtt caccatgtcc
    13381 cgcgcgtaag ccttgtcaaa agcatcgccg gaaagggcgg ataggcggtc gtacgttgcc
    13441 tgatcctgtt tgctcaaggt cgtaggcaat tttatcttct gctcgtcggc gacgctcttc
    13501 agcttctcgt tcgcttccga gtggtcttgg accatcttct cgccgaagct ttttactgtt
    13561 tcgtttgagc ctttttcctg cgcgagtttg cccattttca cctcggccat gcctccggcc
    13621 gcggcctctt ttgcgaagtg cgtgtcggcc atcgtgttcg ttgcggaatc tttggagctt
    13681 gtggacgatg ccgtcgtttc ctggccaagc agcagccccg ccgcgaacaa tgaaacgccc
    13741 gcaaagacaa ctagaaaagt gactttccgt attgtctcga tcttcatggt actggctacc
    13801 tccgaagcct caaccctagg ggaccgggca agcggccgca atcaagcgca ggctgtagaa
    13861 ttcggaggcg aatgctgtag cggcacggcg ctgcaaatcc gtcagtcagc ggcgattaca
    13921 ggtcaaaacc gagctgcagc ttcgcagctt cggacataag gctggggttc cacggtgggt
    13981 cgaacacgat ttccacgtga acttccttca catccggcag attcccgagc ttgttctcga
    14041 catcggcctt gaggacgttg cccattccgc agcccggtgc cgtcatggac atcttgatgt
    14101 cgatccgatg cccaccctga tcaagcggcg cagtcacgca ggagtagatg aggcccagat
    14161 cggcgatgtt taccggtatc tcgggatcga agacggtctt cagttgatcc cagaccattt
    14221 tctcattgaa accgccttcc ggagcgcccg gcgccccctc ggcaggggca acgggcggcg
    14281 tgaggcccag cgcatccgcg tcctttacat ccacgcggaa catgccgtgc gggctcgtta
    14341 ccgtccagct gctgccgaga aactgcatca cgcgcacgtc ggtgccggag ggaagcacac
    14401 cctttgtgcc gctcggaatt tcaatagcgt cgcaatcgcg gcttagtgta atgatttcgg
    14461 tggccatgtt gctcgcctat gcctgagaga ttactctgtg gagaccggct cctgcttgtc
    14521 ctcgagggcc gcctgcagcg tatgccacgc tagcgtggcg cacttcacgc gaaccgggaa
    14581 ctcagaaacg ccggaaaata ccgtcaattt gcccagctcc ccgtcgccgt tcgaggattg
    14641 ctggcccgtt acgagcttat ggaaacgctc gaagattttc gacgcttccg ccgtgctttt
    14701 gcctttcaag ctttgcgtca tcatcgacgc cgaagccttc gagatggcgc agccggaccc
    14761 ttgaaacgtg atatcgcgaa tcgaatcgcc ctcggtggtc acgtaaatcg tgaaccggtc
    14821 gccgcaaagc ggattgaagc cctccgcctt atgattcgcc gccggcagtt cgcggtaatt
    14881 cctgggcgct ttgctgtgct ccagaatcac atcctgatac aaatcgcgca gttcactcat
    14941 caggcgaaca cctcgcgcac ctttccgatt ccgcgaacca gcgcgtcgat ttcctcaaaa
    15001 gtgttgtaca ccgcaaacga cgccctcgcc gtcgccggga ttccgaatcg ctccatcacc
    15061 ggctgcgaac agtgatgccc ggtgcgaatc gcgattcctt cctggtcgag aattgttccc
    15121 acatcgtgag gatgcacgcc ttccaccacg aacgagatca cgcccgcccg ttccttcgcc
    15181 gttccgatca ggctcactcc cggaatcgcg gaaaccttct ccgtcgcata ggcgagcaac
    15241 tcatgctcgt gcgcggcgat gttctcgatt cccagtccgt tcacatagct cagcgcagcg
    15301 ccgagcccaa tcacccccgc tatatcggga gtgccggctt cgaacttgtg tggcagcttg
    15361 ttgtacgtag tcttttcgaa ggtaacggaa ctgatcatgt cgccgccgcc ttgataggga
    15421 ggcatcgcgt tcagcagcgc ggacttccca aaaagcacgc caattcccgt gggaccgtac
    15481 accttgtggc ccgaaaaagt ataaaaatcg cagtcgagcg cctggacgtc cacttttata
    15541 tgcggcgccg cctgcgcgcc atcaatcagc actggaattt tcttgccatg cgccagctcg
    15601 atcatttgct tcaccggatt gaccgagccg agcgcatttg aaacctgcgt gaacgctgcg
    15661 agcttggtgc gcggcccgag aagccgggca aactcatcga gcagcagctc gccctggtcg
    15721 ttgatcggcg ccactcgcag ctttgctcct cgctcgtcgc acagaatctg ccacgggacg
    15781 atattcgaat ggtgctccat ggcagtgata agcacttcat cgccctggcg aatctgcgcg
    15841 cgcccgtagg tttgcgccac caggttgatg ccttccgtgg ttccgcgaac gaaaataatc
    15901 tcgtgcgcgt gcgctgcatt gataaatttc tgcgcggttt ggcgggccgc ctcgtattcc
    15961 gccgtggcct gttcggaaag aaaatgcact ccgcggtgga tattggaatt ggtcccctcg
    16021 tagtaccgcg tgatcgcatc gatcacggcc cgaggcttct gcgaagtggc cgcattatcc
    16081 aaatagacca gcggcttgcc gtgaaccgtc tgatggagaa tcggaaaatc ggcgcgaatc
    16141 ttttcaatgt ccaggccgct agtgtgtgtt tgcgcgctcc catcgcggcc cctcgcgggc
    16201 gctcgcggcc gggtttcgag tttattcgcg gctgtgctca tcgtttctga tctctctgtt
    16261 ccattggatg cgatgtgccc gccatgcgaa gcgcctgttc ttgaaccagc ctttccacgt
    16321 ggcttcgcac cggcccttct ttcattcggt cgagacattc gctcgcaaaa gcgaccagca
    16381 gaagcgttcg cgcggaggct tcatcaattc cgcgagaccg cagataaaat agcgcgttct
    16441 cctcgatctg cccgattgtc gcgccgtgcg tgcacttcac gtcgtccgca taaatttcca
    16501 gctgcggctt ggtatcgatc tgcgccgtat cggaaagcag caggttgcga ttggtctgct
    16561 tggcgtccgt cttctgcgcg tccttgtgaa cgatgatgcg cccgtgaaaa acgccgtgcg
    16621 cgcggtcacc caagatgccg ttgtaaaatt gcctgctcgc gcaatgcggg ctcgcgtgtt
    16681 ccaccagcat gtaattgtcc aggtgctgcc gcccattgcc gataaagagt ccgttaatca
    16741 agcactcgcc gccttcgccc ctcagcaccg gatggacgtt gtttcgaacc agcgcgccgc
    16801 cgatcagaac ggaatgtgag gccacatcgg cgctccgccc ttgctgaatt cgcagcgtgg
    16861 agacgttgta ggtcttcaca tgctcgcgct cgatcatgta gtgcgatacc acgctgtgat
    16921 ctcccgccac taattccgtt accgtattgc acagcgcctc gcctccgccc agcgatacgt
    16981 agtcttcaac gatggtcagt tggctgttct cttctgccac gatcaaattg cgggggtaac
    17041 ttaccgtagg cgcatcgtgc ccggttgaaa caaagagcag cgcgatcggc tcttccgcca
    17101 ccgtgccttt ggccaagtga acgaatgcgc cgtcctccaa aaacgctgta ttgagcgccc
    17161 cgaaagcatc ccgctggaga tcgaggtatc ggccaagata aggttcaact gcgcctggat
    17221 tgcgcgcgag ttccgctgcc aggctcgcca cagtgacccc gacgggcagc ttaccgattg
    17281 tcgaaagctg cggcgcaaaa cgcccgtcga caaaaacggc ctggcaacct gctccgggca
    17341 ctcgaaatgc tgccagttga tctttagagg gaagggaatg gccgttgcgg acgagccgaa
    17401 aatgagtctg tgcgattgcc gcgggattgg tgaaccgcca gtcctcgtca cgtgtggtcg
    17461 gaaatcccgt ttcgctgaag cgcgcgaagc cgctttgcct cagctcgcgc agccaggata
    17521 attcccggcc cgcagcggac ttctcgaagc cgccgaaggt ttcctcgtaa ttttgcaatc
    17581 ccgcgctcgc tgttgccacg ctctcgctcc ccctaaattc ttcacagccg gattaagcgc
    17641 ccgctgccgc ctcaaacgcg ccgtaacctt tatcttcgag gtccagcgcc agttcgggcc
    17701 ccccggactt cacgatgcgg ccatccacca gcacgtggac ataatcgggc acgatgtagt
    17761 tgagcagccg ctgatagtgc gtcaccacca gcatggtgcg gcccggattt cgcatagcgt
    17821 tcacgccttg agccacgatc ctcagcgcgt cgatatccag ccctgagtcc gtctcgtcca
    17881 gaatcgccag cttcggttcg agcacggcca tctgtaggat ttcgttgcgc tttttctcgc
    17941 cgccggaaaa agcctcattc accgaccggt tcagcagctt ctcatccata tcaagcagct
    18001 tcagcttttc gcgcagtttg ggcagaaaat ccatcgcgtc catctcttcc tggccccgat
    18061 atttgcggat cgcgttgagc gccgagcgaa gaaagtaggc gttgctgatg ccgggaattt
    18121 cgacgggata ctgaaacgcc aggaaaagtc cctcgcacgc cgcctcctcc ggtttcatcg
    18181 ccagcaggtc cttgccttcg aagagaactt cgccccccgt gacctcatac gtctcgcggc
    18241 gagccagcac ttgcgctagc gtgctcttgc ccgagccatt gggccccatg atcgagtgaa
    18301 cttcgcccgc gttcaatttc aggttgatgc cctttagaat ttcgcgggca tccaccctga
    18361 ctttcaggtc cttgatttcc agcaaactca ttcgcttcgt cctttgtatc gccttagcta
    18421 cccgcttttg tgacggtaga ctgttacgcc atcctttgtg ttctgtaact tacagcggtt
    18481 tctactcggc tgggctgtcg aatagaagcg gccaggccgg ttatccgacg cttccttcga
    18541 ggctcacgct cagaagcttt tgcgcttcca cggcgaattc catcggcaac tcgcggaaca
    18601 cgcgcttgca gaagccattc acgatcatgg agacggcatc ctcggccttc aggcctcgct
    18661 ggcgaagata gaacatctgg tcctcgccga ttttcgaagt ggaagcctcg tgctccatcc
    18721 gcgccgtcga atttttcact tcgatatagg gaaacgtgtg cgccccgcac ttgtcgccga
    18781 tcagcaggga atcgcactgg gtgtaatttc ttgccccggc cgcgcccttc atgatggtta
    18841 cctggccgcg ataggtattc tggccgtggc ctgcggaaat gccttttgac acgacggtgc
    18901 ttcgcgtgtt tttgccgatg tgaatcattt tcgtgccggt gtccgcttgc tggtagttat
    18961 tcgtaagcgc gaccgagtag aattcgccca ccgagttttc accctgcagg atgcagctcg
    19021 gatacttcca agtgatcgcc gagccagttt ccacctgcgt ccaggaaatc ttcgaattct
    19081 tgccgcgaca ggcgcctcgc ttggtgacga aattgtagat tccacccttt ccgttcttgt
    19141 cccccggata ccagttctgg accgtggagt atttgatttg ggcgttatcg agagcgatca
    19201 attcgacgac tgcagcgtgt agctggtttt cgtctcgaat gggcgccgtg catccctcca
    19261 agtagctgac cgaggcgcct tcgtccgcaa taatcaaagt ccgctcgaac tgtcccgttt
    19321 ccgcggcatt gatgcggaag tatgtcgaga gctccatcgg acatcgcacg cccttcggca
    19381 catagacgaa tgatccatcg gtgaagaccg ctgcgttcag cgaggcaaag aagttgtcag
    19441 tgtaagggac cacgctgccg agatactttt tcaccaactc ggcgtgattt tgcaccgcct
    19501 cggaaaacga acagaaaata atccccagtt cagcgagctt gctcttgaaa gtcgtggcca
    19561 cggagacgct atcgaacacc gcgtccaccg caacgcccgc gagcatcttt tgttccgcga
    19621 gtggaatgcc cagcttttca tatgtccgca gaatttcggg atcgagctca tccaagcttt
    19681 tcagattggc cttctgcttc ggcgcggaat aatacgaaat ggcctgatag tcgatgggtg
    19741 ggtagtggac attcgcccat ttcggttcgg cctgcgcttt ttccagcgaa tcccagtgct
    19801 tgtaggcctt cagtctccat tcgagcatga actccggctc gttcttcttc gtggagatca
    19861 gccgaataat atcttcgttc aatcccttgg gcgcacgatc ctcgccgata tcagtgacga
    19921 atccccactt gtattcctgc tgcgccagtt cccgaatcgt atcggtattc gaactcattg
    19981 aactctcccg gacgcgatca ccggcaagac tttgccgcgc gcgtcctcga tcgtcgtcag
    20041 ttgaagcggg tgcatcaggt ctgaaagtgt gaccttttca agcacgcttc tgacggctcg
    20101 attgatgatt tgctgattgc ttttaatagg gcagtaagac tcaaggttgc acaagccggt
    20161 tatgtctgtg ctgcactccg tgagtgccat cgggccttca atcgccgcga tcatttccag
    20221 caccgaaatg tttcgcggct cccgcgcgag cacgtagccg cctttggtgc cgcgatgcga
    20281 aatcagaagc ccgctctgca gcagctcttt gagcagcttg ctcaccgtcg aaataggcaa
    20341 tttggattcc cgcgccaaat cccgcgccgt ccgcagcgga gcgccctgcg tccgcgcgat
    20401 acaggtcatc agaaccagcc cgtagtccgt taatttacct agccttacca ttgcatgccc
    20461 acctcagagc tatacaggac taacttggta caatttgatt gtagcagcgt acggcagtgg
    20521 gttgcaaggc ccgaaaaatg ccgggctgca ggccgcgaac gagcttcgaa ataggactat
    20581 atagaatgac aatattgatg gattttct
//
LOCUS       DATNDT010000290         3838 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1629029, whole genome shotgun sequence.
ACCESSION   DATNDT010000290 DATNDT010000000
VERSION     DATNDT010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3838)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3838)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3838
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1629029"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="VN788_09365"
     CDS             <1..1148
                     /locus_tag="VN788_09365"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXU20776.1"
                     /translation="PRSISRRVALMRLPPEIWATNLGAFRVSLPALEHFPAGIWSKLV
                     NRHLRKQSRQLMAYGDAKGYMPFREAIAEYLGAVRAVRCEPSQILITTGSQQGIQLSA
                     QVLLNPNERVWFEEPGYPGARRALLAAGAELVPVPVDRGGLNVAEGIRRARNAHAAYI
                     TPSHQYPLGVTMDATRRMLLLNWAARSGAWIIEDDYDSEYRFGGRPIPSLQGLDNSGR
                     VIYVGTFSKVMFPALRLGYVAVPKDLVDAFATARDAHDQFSSTLYQAVMADFICEGHF
                     ARHIRRMRVLYSQRRTALVEAIQKYAGDKLEVVGAEAGMHLVALLPPGVNDVVISKRA
                     AEMGISVMPLSSCYAKPPARRGVILGYGGTDARQIHDATRKLSTIFR"
     gene            1634..2467
                     /locus_tag="VN788_09370"
     CDS             1634..2467
                     /locus_tag="VN788_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20777.1"
                     /translation="MRRIHFVELHEQPWFPSSIRDEVTDALQFGLNILKAYAPIAPLL
                     QTALDATRSPSIVDLCSGGGGPWLDLSQKLHRNAEHFDILLTDKYPNLGASELVKEAS
                     GNHLLFHPDPVDPRNVPGELTGFRTMFSSFHHFPRDQARAILQNAIDARQGIGIFEIT
                     RRTPSAIGLMFPWAFTALLFTPWIRPFRWSRLFWTYVIPLIPFVLLFDGVVSCIRTYT
                     PKELREIVDELAAIEYRWEFGEHSRAAAQVPITYLIGYPLPHLRSEVSETTTSEKSLA
                     P"
     gene            complement(2480..3349)
                     /locus_tag="VN788_09375"
     CDS             complement(2480..3349)
                     /locus_tag="VN788_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HXU20778.1"
                     /translation="MRKRLLAAAILFALPASAQQSNSPPDSSTDAEPAAQSDYSVKTT
                     LRDFVTDEYRMWSSPFRVSDYDAHTMEKYGFPFLGVSAALIATDQTTSRGLPRAGTQA
                     FIWSGRVSQIGAPYTLAGATATLFFLSKAAHEDHLTETGFLSMDALAHSELIGLVIKE
                     ATQRERPVNVTASISRNGFWDGGQSFYSGHATGAFAVATVVAYEYGGQHIAVPIIGFT
                     LASIVSASRVSGRQHWVSDVFVGSAMGFMEGRYIYKRHHNPDLPGSRVRRASRLIPDF
                     DVGINGGSLYWEF"
     gene            3607..>3838
                     /locus_tag="VN788_09380"
     CDS             3607..>3838
                     /locus_tag="VN788_09380"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU20779.1"
                     /translation="MTPTNPESRRLDVTLDTRLESVDLAESLVMRAAEEAGFAEDEVH
                     KIGMAVREGVVNAYNYGNCQDCQKKIQLTVELD"
BASE COUNT          790 a         1157 c         1047 g          844 t
ORIGIN      
        1 ctccgcgcag catctcgcgg cgcgtggcgc tgatgcgatt gcctccggaa atttgggcca
       61 ccaatctcgg cgcatttcgc gtcagccttc cggctctcga gcatttcccg gcgggaatct
      121 ggtcgaagct tgtgaatcgc catttgcgaa aacagtcccg gcagttgatg gcttatggcg
      181 acgccaaggg ctacatgccc tttcgcgaag cgatcgcgga atatttgggc gcggttcgcg
      241 cggtccgatg cgagccgtcg cagatcctga taactaccgg ctcgcagcag ggcatacagc
      301 tctcggcgca ggttttgctg aacccaaatg agcgggtgtg gtttgaagaa ccaggatacc
      361 ccggagcgcg ccgggctttg ctggcagccg gagccgaact tgttcccgtt cccgttgatc
      421 gaggcgggtt gaacgtagcc gaagggattc gccgcgctcg caatgctcac gctgcgtaca
      481 tcacgccgtc gcatcaatat ccattaggtg taaccatgga cgccacccgt cgaatgctct
      541 tgctcaattg ggccgcgcgc agcggcgctt ggattatcga agatgattac gatagcgaat
      601 acaggtttgg aggccgcccc attccctcgc tccagggttt ggacaacagt ggccgcgtaa
      661 tttacgtcgg caccttcagc aaagtcatgt ttcccgcgct gcgcctcggt tacgtcgctg
      721 tccccaagga tttagtggac gcattcgcga ctgcgcgaga cgcccacgac caattttcat
      781 ccactctcta tcaagccgtg atggcggatt ttatctgcga aggacacttc gcgcgccaca
      841 tccgcagaat gcgcgtgctg tactcgcagc ggcgaactgc cttggtcgaa gcgattcaga
      901 aatacgcggg cgataaactt gaagttgtcg gtgccgaagc gggcatgcat ctggtcgctc
      961 ttctgccgcc gggcgtcaac gatgtcgtca tttccaaaag ggccgcggag atgggaatct
     1021 cggtgatgcc gttatcctcc tgctacgcga aaccgcccgc gcgccgcggc gtgattctcg
     1081 gctacggcgg caccgacgcc cgccaaattc acgacgccac gcgcaaactc agcacgattt
     1141 tcaggtaggg gcacagcgtg ctgtgcccgg caaacatact tggctacttg tgccccatcc
     1201 gcctcgcgcg aacggcgcct cctgtggagt gcgggagctt gtttaccctg cttgcctgac
     1261 gcaggaaggg agcttcgaag cgcttccact gtctgccgct cgggcttgcc cgggcgtgct
     1321 cctcgcattc actacaaccg cccccgtttt gtcttttgct gggagccacg caccgaagcg
     1381 tcgtcattcc gagcggagtt tcgcgcgttt tccctttcgc gcggtgtctg cgcgcgaaac
     1441 gcagttgagg aatctctctt cgatgtaaca cccgcagccg tgcccatttg cgtggcacct
     1501 tggtacgggc acgatatatc gtgccgggca aacatgcttg gctacgtgca agccatggat
     1561 aaccgtctcc aggccgctac ttctattggc cattgaacgg attttctgtt acgactcacc
     1621 aggccaagcc gctatgcgtc gcatacactt cgtcgaactc catgaacaac cctggttccc
     1681 gtcgtctatt cgagatgaag ttacagacgc gcttcaattc ggcttaaaca tcctgaaggc
     1741 ctatgcgccc atagccccgc tgctccaaac cgcgctcgac gccacgcgga gtccatcaat
     1801 tgtcgatctc tgttcgggcg gcggaggccc ttggttggac ctttctcaaa agctccatcg
     1861 caacgcggag cactttgaca ttctcctcac cgataaatat ccaaacctcg gcgcatccga
     1921 acttgtcaaa gaagcgtctg gaaatcatct gcttttccat ccggacccgg ttgacccgag
     1981 aaacgttccc ggtgaattga ccggattccg aacgatgttt tcctcattcc atcattttcc
     2041 gcgcgatcag gctcgcgcca tactccaaaa tgccatcgac gcccgtcaag gcatcggcat
     2101 atttgaaata accaggcgca caccgtcggc cataggcttg atgttccctt gggcattcac
     2161 ggcattgctt ttcactccgt ggatccgtcc cttccgttgg tcgcggctgt tctggacata
     2221 cgtgatcccg ctaattccat tcgtcttgct gttcgacggc gtggtgtctt gcattcgtac
     2281 ctacaccccg aaggaattgc gtgagattgt cgatgaacta gcggcaatcg aatatcggtg
     2341 ggagttcggc gagcactcgc gcgcggccgc tcaagtgccg attacttacc tgatcggcta
     2401 tcctctgccg catttgcgat ctgaggtgtc ggaaactacg acgagcgaaa aatcgctcgc
     2461 cccctgaaga aaagaggcct caaaattccc agtagaggct tcccccgttg atgccgacgt
     2521 caaagtcagg aataaggcga gaggcacgac gcacgcggct cccgggcaga tccgggttgt
     2581 ggtgacgctt gtagatgtat cgcccctcca tgaatcccat ggcgcttccg acgaacacat
     2641 cggagaccca atgctgccgc cccgacactc gcgaagcgct cacgatcgaa gcgagcgtaa
     2701 agccgatgat cggcaccgcg atgtgctgcc ctccgtactc ataggcaacc acagtagcaa
     2761 ccgcgaacgc acccgtcgcg tgtcccgagt aaaacgattg ccctccatcc caaaacccat
     2821 tcctgctgat cgaagcggtc acgtttaccg gacgctctcg ttgggtcgcc tcttttatca
     2881 cgaggccgat gagctcgctg tgagccagtg cgtccatgct caaaaatccg gtctcggtca
     2941 aatggtcttc atgtgctgct ttgctgagaa aaaaaagagt cgcggttgcg ccggctagcg
     3001 tgtacggagc gcctatttga gaaacccgcc cgctccaaat aaaagcttga gttccggctc
     3061 tcgggagccc gcgtgacgta gtttgatcgg tcgcgataag agcggctgaa acaccgagga
     3121 aaggaaaacc gtatttttcc atggtgtgcg cgtcgtagtc gctcactcgg aatgggctgc
     3181 tccacatccg atattcatcg gttacgaaat ctcgcagcgt agttttgacg ctgtagtccg
     3241 attgtgctgc cggttctgca tccgtggagg aatccggagg tgaattggat tgctgtgccg
     3301 acgcagggag agcgaaaagg atagctgcag ccagcaatcg cttacgcatc tcgcaaagat
     3361 gcacctgccg cctgcgtccc gccatacaac ttttgctgta tcggtttgag cggaaaccac
     3421 tagcgccatt tgtcacaatc aaattcggct tgtccggacg cccaaaatct ccgagcaggc
     3481 ggcctcagat gagtagtgca tattcagtac tagcctactg ttaaattccc atcgcttccc
     3541 aatgggccag tagattgacg ccgtcggtcg cgctatgata cccatactca gcatgttgag
     3601 gccctggtga ctccaaccaa tccggaatcc cggcgtctgg atgtgacgct cgatactcgc
     3661 ctggaaagtg tggacctggc cgaaagcctc gtgatgcgcg ccgccgaaga agcaggcttc
     3721 gccgaggacg aggtccacaa aatcggcatg gccgtccgcg aaggcgtcgt caacgcttac
     3781 aattacggaa attgccagga ctgccagaag aaaatccagc tcaccgtcga attagacg
//
LOCUS       DATNDT010000291         3562 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1170540, whole genome shotgun sequence.
ACCESSION   DATNDT010000291 DATNDT010000000
VERSION     DATNDT010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3562
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1170540"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(245..2185)
                     /gene="ftsH"
                     /locus_tag="VN788_09385"
     CDS             complement(245..2185)
                     /gene="ftsH"
                     /locus_tag="VN788_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HXU20780.1"
                     /translation="MNSTVRTVLFWVMMIALAAVLWQMASKGGPTAHEDEPNYTSFIN
                     KVDSGNVKGVTVYLSPNSAELQGEYRDNTKFRGVTVANAAIPDVTKTLQDKGVPFNYK
                     EVKEANWLSILITLSPFLVIIAIWIFMIRQMQAGGNKALSFGKSRARLLTAQQKKATF
                     KDVAGIDEAKEELYEIIDFLKDPQKFQKLGGRIPKGVLLVGPPGTGKTLLARAIAGEA
                     NVPFFSISGSDFVEMFVGVGASRVRDLFEQGKKNAPCIIFIDEIDAVGRHRGAGLGGG
                     HDEREQTLNALLVEMDGFESNEGVILIAATNRPDVLDPALLRPGRFDRRVVVPRPDVK
                     GREEILRVHTRKVPLSDDVDLSVIARGTPGFSGADMANLVNEAALWAARQNRKFVAMV
                     DFEMSKDKVLMGAERRSMILSDEEKKNTAYHEAGHALVAAMTPGADPLHKVTIIPRGM
                     ALGVTMQLPIDDKHTYTKEFLESQLAVLMGGRAAEEIFLNHITTGAGNDIERATEIAR
                     QMVCEWGMSSLGPLTFGKKEEAIFLGREIAQHRDYSEDTAIKIDVEVRTIVNSGYTRA
                     RNILETYRDALERIARALLDREVLDAMELKLLMEGKPLPEKVPPPPPAAPPLPAKEPQ
                     LARRPDPRPVPGLAKGEKPAPA"
     gene            complement(2287..>3562)
                     /gene="tilS"
                     /locus_tag="VN788_09390"
     CDS             complement(2287..>3562)
                     /gene="tilS"
                     /locus_tag="VN788_09390"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HXU20781.1"
                     /translation="LGLECLVERGDVLAAVSKNKWNLEDAGRRLRYAFFERLVAEGKA
                     NRIAVAHTEDDQAETVLAHLLRGTGPTGLAGIHPERDSTIRPLLGERRATLRQYLREI
                     DQPWREDSSNLDMRRGRARIRERLLPVLERDFTNHITNRLATLAELAREEQLFWKVLV
                     EDGLETLVHRDEGKLSIAIGDLLAPIDALVQRKTRTGGQKEHWQRALTERLVRRLYED
                     VHGTRFGLGLGHVKQVIHLASESASGSRVGLPGGVTVGREFDELTFSRVSHKDGRRGA
                     MKAVGSMGYGYTIDLPIQGTASISVPVLKRCFSLKMIDWSIAERDTKRGCVALDADLL
                     RSPLILRNWRSGDRYRPLGRRHTAKLKEMFRLRKVPSGERLLWPVLESAGNVIWAGGM
                     PVAHEFCAGEATRLGLLIDEAAGTISELRRGI"
BASE COUNT          693 a         1133 c          940 g          796 t
ORIGIN      
        1 cggtcttaca ggcgcaacgt gcgccagcac cgctccaagt tcccggtcca cggtcatctt
       61 cgctccgcag caggggcaat caatttcgaa tacttcctgt cggcggttcc ccatgtcgat
      121 attctacgcc agttctcgca agacgaaggc caggagtcct tgtggagaac ctgacccttc
      181 gcacaaaaga aaagaggctc gccacggaag ccgagcctca ggagtcgaag ccaaccgcta
      241 cgatctaggc tggcgccggt ttttcgccct tggccagacc cggcacgggc cgcgggtcgg
      301 gtcgccgcgc gagttgcggc tcctttgccg gcaagggggg cgcagcagga ggtgggggtg
      361 gcaccttctc cgggaggggt tttccctcca tcaggagctt tagttccatc gcatcgagca
      421 cctcgcggtc gagcaaggct cgcgcaatac gttcgagggc atcgcggtac gtctctaaaa
      481 tgtttcgcgc ccgggtgtac ccgctgttaa cgatggtgcg gacttccaca tcgatcttta
      541 tcgcggtgtc ctcggagtag tcgcgatgtt gcgcgatttc tcggcccagg aaaatcgcct
      601 cttccttctt gccgaaagtc agcggcccca gcgaactcat tccccactcg cacaccattt
      661 gccgcgcgat ttcagtggcc cgctcgatat cgtttcccgc tccggtggtg atgtgattaa
      721 ggaaaatttc ctctgcggcg cgccctccca ttaagacagc cagctgcgac tccaggaatt
      781 ccttcgtgta tgtgtgcttg tcatcgatcg gcaattgcat tgtgacgccg agcgccatgc
      841 cgcgcggaat gatggtcacc ttgtgcagtg ggtcggcgcc cggcgtcatt gccgccacca
      901 gcgcatgccc ggcttcgtga taagccgtat ttttcttttc ctcatcggac aagatcatgg
      961 atcgccgttc agcacccatc agcaccttgt ccttcgacat ctcgaagtcc accatcgcta
     1021 cgaacttgcg gttttgtcgc gctgcccata acgccgcttc gttcacgagg tttgccatgt
     1081 ccgcgccaga aaatcccggc gtgccgcgcg caatgacgga aagatcgacg tcgtctgaga
     1141 gtggcacttt tcgcgtgtgc actcgaagaa tctcttctct ccccttcacg tcgggccgag
     1201 gcacgaccac acgtctatcg aagcgaccgg gccgcagcag cgccggatcc agcacgtccg
     1261 ggcggtttgt ggccgcaatc agaatcacgc cttcgttcga ttcgaaaccg tccatttcga
     1321 ccagcagagc gttcaaagtt tgctcgcgct catcatgccc gccgccgaga ccggcgcctc
     1381 gatggcgtcc caccgcgtcg atttcatcga tgaaaatgat acagggcgcg ttcttcttac
     1441 cctgttcaaa gaggtcgcgg acgcggctcg cgcccacgcc gacaaacatt tccacgaaat
     1501 ccgatccaga gatagagaaa aacggcacat tcgcttcgcc ggcaatcgca cgtgccaaca
     1561 acgtcttgcc ggttcccgga gggcctacga gcagcacgcc tttaggaatt cgcccgccaa
     1621 gtttctggaa cttctgtgga tccttcagga agtcgatgat ttcgtagagc tcttccttgg
     1681 cttcatcgat tcctgcgaca tccttgaacg tcgccttctt ttgctgcgcc gttagcaaac
     1741 gcgcgcgaga ctttccaaaa ctgagagcct tgtttccccc tgcctgcatc tgccgaatca
     1801 tgaatatcca aatggcgatg atcactaaaa agggcgaaag ggtgatgagg atactgagcc
     1861 agttggcctc ttttacttcc ttgtagttga aagggactcc cttgtcctgg agcgttttcg
     1921 tcacatcggg aatggccgca ttcgcgaccg tcacgccgcg gaatttggtg ttatcgcgat
     1981 actcgccttg aagttctgcg ctattcggcg agagatagac agttacgcct ttgacattgc
     2041 cactgtccac cttgttaatg aaactcgtgt agttgggctc atcctcatgt gcggtcgggc
     2101 ctcctttgga agccatctgc caaaggaccg cagccagcgc aatcatcatt acccaaaata
     2161 aaacagtgcg taccgttgag ttcacgtctt acctcgcccc gggcctttga atagattcga
     2221 gacttccgcg ttacccgtcg tcgctccatc gcgaaaaacc atacgagccg cctatttcca
     2281 cttatgttag atgccgcgac gaagctccga gattgtccct gcggcttcgt cgatcagcag
     2341 acctagccgt gttgcttctc cggcgcagaa ctcatgggcc accggcatcc ctcccgccca
     2401 gatcacgttc ccggcgcttt ccagcaccgg ccaaagcagg cgctcgccac tcggaacctt
     2461 ccgaagacga aacatctcct tcaatttggc cgtgtgccgc cggcccagcg ggcgatacct
     2521 gtctccggac cgccagttcc gtaggatcag gggagagcga aggagatcgg catccagagc
     2581 tacgcagcct cttttagtgt cgcgctctgc gattgaccag tcaatcattt tcaagctgaa
     2641 gcatcgcttc agtaccggga ccgaaatgct cgctgtgccc tgaatcggaa ggtcaattgt
     2701 gtacccgtat cccatcgagc cgaccgcctt catcgcaccc ctgcgtccat ctttgtgcga
     2761 cacccgcgag aaggtcaatt catcgaactc gcgcccgacc gtaacgcctc ctggaaggcc
     2821 cactcgactc ccgcttgcgg attcgctcgc aagatgaatc acctgcttta catggcccaa
     2881 gccaaggccg aagcgggttc catgcacgtc ttcataaagg cgccggacca gtcgctccgt
     2941 aagcgcccgc tgccagtgtt ccttttgccc acccgtcctg gtcttccttt gcacgagggc
     3001 atcgattggg gccagcagat ccccgattgc gattgataac tttccttcat cccgatgaac
     3061 caatgtctcc aggccatcct ccaccagcac cttccaaaaa agctgctctt cgcgagcaag
     3121 ctccgccaat gtagcaagcc ggttcgtgat gtgatttgta aaatcgcgct cgagcacggg
     3181 cagcagacgc tctcggattc tcgctcgccc gcgacgcatg tcgagattgc tcgagtcctc
     3241 gcgccacggt tggtcgattt cccggaggta ctggcgcaaa gtggcgcgtc gttcgccaag
     3301 aagcggtcgg atggtggagt ctctctccgg atgaataccg gcgagcccag tcggacctgt
     3361 cccgcggagc aagtgcgcga gcactgtttc cgcttgatcg tcctccgtgt gtgcgacagc
     3421 gatgcggttg gcttttccct ccgcaaccaa ccgctcaaaa aaggcgtatc gcaaccgccg
     3481 gccggcatct tccaagttcc acttgttttt tgaaactgcc gctaggacgt cgccgcgctc
     3541 caccaggcat tcgagtccca gg
//
LOCUS       DATNDT010000292        10407 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4166327, whole genome shotgun sequence.
ACCESSION   DATNDT010000292 DATNDT010000000
VERSION     DATNDT010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10407
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4166327"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3234)
                     /locus_tag="VN788_09395"
     CDS             complement(<1..3234)
                     /locus_tag="VN788_09395"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXU20782.1"
                     /translation="MLNKRHIARRVLIAIPAVLLGLALLCVIVLNSSMFRNFVRSEIR
                     KQAFERAGMRIEIGALKMHWSRLGLEAERITIRGTEGRMTQEPALFQAKRLDLSIRFL
                     PLLRGKIQLGEFVLDQPVLRLRIDSQGRSNLPSARRPSTKSVPDTIFDFDVQNCAIHS
                     GEIYYNDARVPLDADLHDLKFESRSRPLTGEYAGSLSYDKGHLTARQSYNVTHAAQLE
                     FVASRSGLVIKRLLLTSGASHFAFSGHLTNYASPRVEGAYEGNLFTNEVGKAIGSDSL
                     PAGDVGLDGKFTYHASGQQPLLKMLEVQGRMQSRALTVRTSNGSLAASAISANYQLKD
                     ANLRVQNFTAEILGGHAQANWQMLNLVAPAPLNRLDASVQGVSLHRASDMLSARATQK
                     IPLIGTMNMKARASWSGSIDSAVAQVRLAISNPQSVSAASTRIPVNGMVKADYDGPAH
                     TISFGQSYLQTHATRITVSGTLGSRRGDSSNLNVLATANDLGEIRSLVMLIQNAMPSA
                     TPTTAIPPLGGNATFDATVGGSAKDPQIRGRLTAQDFSVDHSHWRSLSLNLAARSSQV
                     AIQNGVLVAGPREQISFSGGSTLRKWSIPPGAPIQLQVAAANLPVADIADAAQLHYPV
                     TGTVSAHVSIKGTREAPEGNATITLTRGSAWNEAINHLAVDAVFHQGTVKPTINLQIP
                     AGSISANGSYSLQTRAYQLTLHGRAIQLDRISAIERAVPLQGTADISVSGSGTIENPQ
                     LELNLTSAQFQVRNQVISRVAAKVSVGNRHANVTFHSVIDQGAVDAKGAIDLAGSHYT
                     VASLDVRALPVAAVLANVLSAQTAKVGGETALHVDLKGPLEAPAQLEAHLQIPSFNMT
                     YGNAHLELTHPLIADLHNGELIVASTQIRGTGANLTFAGKMPIRGAATRYSVSADGSL
                     DVAVLQTFVPGVRSSGQIKLHLNGQGTSSQPKMLGQLQLKDVALSADASPVGIEGLTG
                     QVNISGDRADIANLSGTVGGGKVLATGFVSYGHETNFNLGLNAQSVRIRYPEGLRSIL
                     SGQINVRGNPDDSTLSGRVLVDRLSFTQAFDLANFAGYFSEDSTGSPP"
     gene            complement(3265..6249)
                     /locus_tag="VN788_09400"
     CDS             complement(3265..6249)
                     /locus_tag="VN788_09400"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXU20783.1"
                     /translation="MKRRLVDVSLWHCARQARYALFWLCPILDLLGGGLLFAQQTQPM
                     SYAGFDGQTVSTVEISARQNVDITAMRERIEVRPGKPFSTRELNESVAALRQTRQFSE
                     IQVSLEPEQAGLEVIFILQPTDYVGVIDFPGTGTKFPYTALLQAVDIPEQSPYFSGLG
                     VKGQQGLLAYLHKRGYFSADVQVETSHDETHRVVNVIFHCDLRKQARIRKIEFTGISE
                     QQSEQLKAALKGIWARLKRVTLKPGQKYSEPRIARSIDFIRDRLRSQNRLAPSVRLAS
                     STYVPDSNRVDLRLDVTPGPEVSVRVSGARVSSKTLHRLIPIYEEGAADQDLIDEGQR
                     NLRAYFQTKGYFDAKVDANVNRASEKLEVIYKVERGAKHHEAGVYFDGNHYFSDDELK
                     SNVSIKKGFLFLHGRYSEQLLSKSVNSLTQLYKDQGFENVSIKPKVEDFEPQISVTFE
                     ITEGPQDKVGALHVVGNKTQSLPALGRKYALQLQPRHPFSRKLMEADRTRLLAAYLDL
                     GYPDATVRSAASRAPGQPHTVDVTYTIEEGTQAHVSDVVFLGAKHTKVSFIKEITFAQ
                     LKPGQPLSEGHFLQSESDLYDLGIFDWTSVKPLRPVVNQSQEEVLVKVHESPLNSMDI
                     GGGLEIIPRSGNIPVNSVAVPGIPPISLGNKFTVSQKSYVGPRFSFAFARHNLRGRAE
                     TATIGTVLSRLDQRGFFNYADPHLHGSTWSSLFGVSGERNTQNPIYTAELGQVSFQVE
                     KALDKKHTKNAILRYSFQRTNLYNVLIPGLVLPQDRHVRLSTFDGEYIRDTRDKPLDA
                     HRGVYQTFDFGVTSKSLGASADFVRFLGQSAFYVPVKPSLVWATNIRLGFATPFSGSD
                     VPLSERFFTGGADSLRGFPINGAGPQRPVPVCSNPSNPATCTLISVPVGGNMLFILNT
                     EARFPLPIKQGLGGVFFYDGGNVYSKISLSQFANDFTHSVGAGLRYQTPVGPVRFDFG
                     YRITSVPGIKATQYFVTLGQSF"
     gene            complement(6246..7091)
                     /locus_tag="VN788_09405"
     CDS             complement(6246..7091)
                     /locus_tag="VN788_09405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXU20784.1"
                     /translation="MSRYLLRSSAVLKRIWNRLQLDDCFDLAAQTSFYFILSFFPFCL
                     VLAVIVGWLPYSALWKTFVTWVVTYLPRSSQELVFSTILGLVNYSAGFLSFGFVMTLW
                     SASAGFVSLMESLSIAYGVRDSRSYWRKHAIATVITILAAMFGLGAFGLAAFGQWESK
                     ILAVNMGPPMVSRAVWEIGRWIATLALMFLGIDLLNFLLPDVRRRWRWLTPGTLFAVL
                     TLMALSAGFNIYFRHFSLYPRVYGALGGFIILMLWIYSACLIVLVGAETDDEMEKARN
                     GATVR"
     gene            7412..9301
                     /locus_tag="VN788_09410"
     CDS             7412..9301
                     /locus_tag="VN788_09410"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU20785.1"
                     /translation="MKRLATLSASVATAVLALVFLSPRSIAQGRVAVAKPPGDAVYRK
                     SCAKCHSSAAASNRAPDLKALMKLSPETIYSAITTGAMATAAQQLSPSDKQVLATYLG
                     GRPLDLTGAGSAEHMRNHCEANASFENISSRPAWNGWGVNLANTRFEDAQGAAMTAQQ
                     VPHLKLKWAFGFPNGDTSFGQPTIVGGRVFVGSDIGYFYALNAETGCVYWSYRAQATV
                     RTAAAVGPIADHGTTKYAVYFGDFRGNVYALDASTGKALWIKNVIDNNYVARLSAGPT
                     LYKGRLYLPISSTEEVFAADPSYACCTFRGSIVALDANTGQTVWNSYTIPEAPKPVRK
                     NVRGVQLYAPAGGALWDSPTIDAERQLLYVGTGDAYTEPAPKTTDAIVALDLKTGRLV
                     WSFQAVKDDAWILACVPTPTDNCPKNLGPDHDIGASPILVKMPDGHRLLIALPKSGTI
                     FALDPDRKGALVWKLATTQTVAPNNGQLALGGASDGQNLYVALEDGTAVAVDLASGKR
                     KWLARLESLDELGEPTSNGENRTKAGLRFGQSAAVTAIPGVVFTGGWDGILRALSASD
                     GRLLWQYNTAHAFKAVNGIPAKGGSMGGPGPTVVNGVLYVPSGYAAFGGGMPGNVLLA
                     LSPGE"
     gene            9366..9905
                     /locus_tag="VN788_09415"
     CDS             9366..9905
                     /locus_tag="VN788_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011445515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU20786.1"
                     /translation="MGLVNFGQPDNGIIQTAYIVKDVREAINRWVKDLKVGPWFLLEH
                     FTGEEAKYRGGPSSADVAIAMSFAGHMMIELIAPNDDAPSVYRETIEKRGYGFHHWGV
                     ATSNFDREVEQHRAQGHELAFFARVPSGGRVAYMDTTADLAGMLELIELGASFDPIFS
                     RFYGATIGWDGKHPIRSFL"
     gene            complement(9928..>10407)
                     /locus_tag="VN788_09420"
     CDS             complement(9928..>10407)
                     /locus_tag="VN788_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HXU20787.1"
                     /translation="TEEFTFAGFLPSRPTERRKALRELATEPRTLALYEAPHRLLDTL
                     EDALEIFGNRPAVMAREITKAFEEFSRGHLEDLVRAARTKSPRGEITLLIAPPDGQAQ
                     HTGNEQAPADTVPLARHVEDIMRERGLDRKAALKQAARERGLTRREAYKQLLITRDE"
BASE COUNT         2320 a         2824 c         2947 g         2316 t
ORIGIN      
        1 aggagggctg ccggttgagt cctcagagaa ataccccgcg aaattcgcca agtcaaaggc
       61 ctgcgtaaaa gaaagacgat ccaccaaaac ccgccccgaa agagtcgaat catcggggtt
      121 tccacggacg ttgatttgcc cactgagaat cgagcgcagt ccttccgggt aacgaatgcg
      181 aacggattgg gcgttcaagc cgaggttgaa gttcgtttca tggccatagg agacaaaccc
      241 cgtcgccaaa actttccctc cccccacggt gccagataag ttagctatat cagcgcggtc
      301 accggagata ttgacttgtc ccgtcaggcc ctcaatacca accgggctcg cgtcagcgga
      361 aagggctaca tctttgagtt gaagctgtcc gagcatcttg ggttgagaag aagttccttg
      421 gccgttcagg tgaagtttga tctgcccaga tgagcggaca ccgggcacga aagtctgcag
      481 caccgcgacg tcaagtgatc cgtcggcgga aacagaatat cttgttgccg caccacggat
      541 gggcattttg cctgcgaagg taagatttgc acctgtgccg cgaatttggg tcgacgccac
      601 gatcagttcg ccgttatgaa ggtcggctat caacggatgc gttaactcca gatgggcgtt
      661 cccgtatgtc atgttaaaac tgggaatttg aaggtgagct tcgagttgcg caggagcctc
      721 gagaggaccc tttaggtcta catgaagcgc tgtctcgcca cccactttgg cggtttgggc
      781 tgaaagcacg ttggccagga cagccgcgac cggaagggcg cgtacatcaa gcgaggcgac
      841 ggtgtaatgg cttcccgcaa gatcgatcgc tcccttcgcg tcaaccgccc cctgatcgat
      901 gacggagtga aacgtaacgt tcgcgtgtcg attcccgact gaaaccttag cagcgacgcg
      961 agaaataacc tggttccgaa cctggaattg agcggatgtg aggtttagtt caagttgagg
     1021 attctcaatc gtgccgcttc cgcttacgga aatgtcggct gttccttgaa gagggacggc
     1081 ccgttcgatc gctgatatcc tgtcgagctg aatcgccctg ccgtgcaggg tgagttgata
     1141 tgctcgggtt tggaggctat agctgccgtt cgcggaaatc gagccggccg gaatttgcag
     1201 attgattgtc ggtttgacgg tgccctgatg gaacaccgcg tcaacagcta gatgattgat
     1261 cgcttcgttc cacgcggacc ctcgcgtaag cgtgatggtt gcgttgcctt cgggggcttc
     1321 tctcgtccct ttgatggaaa cgtgagccga aacagtccct gtaaccgggt aatgcagctg
     1381 ggccgcatct gctatatcag caacgggtag atttgcggcc gcgacttgca attgaatagg
     1441 cgcgcctggg ggaatagacc acttccgcaa agtgctgccg ccgctgaaag agatttgttc
     1501 gcgcggacca gcgacaagca caccgttttg aatcgccacc tgagacgatc gtgccgcgag
     1561 attcagggag agtgagcgcc agtggctatg atcaacactg aaatcttgcg ccgtcagccg
     1621 accccggatt tgcggatctt ttgccgaccc accgacagtg gcgtcgaatg tcgcgtttcc
     1681 gccaagaggg ggaattgcag ttgttggcgt cgcggaaggc atcgcatttt ggatcagcat
     1741 gacgagggaa cgaatctcgc ccaagtcatt tgctgtggcg agaacgttga gatttgagct
     1801 gtcaccacgc cgcgagccga gtgttccgga cacggtaatt ctagtggcgt gggtttgcaa
     1861 ataagattgc ccgaaagaga ttgtgtgggc tgggccatcg taatcggctt taaccatgcc
     1921 attcacagga attctggtgc ttgccgccga aacgctctgg ggattcgata tggcgagccg
     1981 tacctgcgca actgcgctgt caattgagcc tgaccatgac gctcgcgctt tcatattcat
     2041 agtgccaatc aacggaatct tttgcgtggc gcgagctgaa agcatgtcgc ttgcgcgatg
     2101 aagtgaaacc ccttgcacgg atgcatcgag ccggttcaat ggcgctggag caaccagatt
     2161 cagcatctgc caatttgctt gggcgtgacc gccaagaatc tccgcagtga aattttggac
     2221 ccgcaagttt gcatctttca actggtagtt ggcgctgatc gcgctggctg cgagcgaacc
     2281 gttgctcgtt cgcacggtta gtgcacggct ttgcatccgg ccctgcactt ccagcatttt
     2341 caacaatggt tgctggccgc tagcgtgata agtgaatttg ccatcgagtc ccacgtctcc
     2401 ggctggtaag gaatccgaac cgattgcttt cccaacctca tttgtgaaga ggttgccctc
     2461 gtaggctcct tcaacgcgag ggctcgcata gtttgtcaaa tggccactga aggcgaagtg
     2521 agaggcgccg ctggtgagca gcaggcgctt gatgaccagg ccagaacgac tggcgacgaa
     2581 ctccaactgc gcggcatgcg taacgttgta cgactgccgc gccgttagat ggcctttatc
     2641 gtaagagaga gagccggcgt attcgccggt caggggcctg gagcgcgatt cgaacttcaa
     2701 atcatgcagg tcagcgtcca atggaactcg cgcatcgttg taatagattt ctcccgaatg
     2761 aatcgcgcag ttttgcacgt cgaaatcaaa aatagtatct ggaacacttt ttgtgctggg
     2821 tcgccgagcc gagggcaaat tgctgcggcc ttgcgagtcg attcgcaagc gaagcactgg
     2881 ctgatccaga acgaattctc cgagttggat cttgcctcgt aaaagcggaa ggaaccggat
     2941 gctcaggtca aggcgtttag cctgaaaaag cgcgggctcc tgagtcatcc ttccctctgt
     3001 gccgcgaatc gtaattcttt cagcttccag gccgaggcgg ctccagtgca tttttagcgc
     3061 gccaatctca atccgcattc cggcgcgttc gaacgcctgc tttcggatct ccgaccgcac
     3121 gaaattccgg aacatacttg aattgagcac aattacgcag agcagagcga ggcccagcaa
     3181 aaccgccgga atggcgatca ggactctgcg agcgatgtgc ctcttattca acatgtcctc
     3241 tttaacccag tcattactcc tgtgtcagaa tgattgacct agtgttacga aatattgagt
     3301 tgctttgatg ccaggcaccg acgtgatgcg atatccaaaa tcgaaccgaa cggggccgac
     3361 tggcgtttgg tagcgaaggc cggctcccac cgagtgagta aaatcgttag caaactgaga
     3421 aaggctaatc ttgctataca cattaccgcc gtcgtagaag aacacccctc cgagtccttg
     3481 cttgatcggc agcggaaatc gagcctcggt attgagaatg aaaagcatat tgccacccac
     3541 cgggacacta atcagcgtgc aggtggcggg gtttgacggg ttgctgcaaa caggcacggg
     3601 ccgttgcggt cctgcgccgt tgatcggaaa tccccgcagc gaatccgcgc ctccggtaaa
     3661 aaatcgctcg ctgagaggaa catcacttcc agaaaatggc gtggcgaatc ccagacggat
     3721 gttggtagcc catacaagcg agggtttcac cggcacataa aatgcacttt gccctagaaa
     3781 tcgcacaaag tccgcgctgg ctccaagcga ctttgaggtt actccgaaat caaaggtctg
     3841 atagacgccg cggtgagcgt cgagcggttt gtcgcgagtg tcgcgaatgt attcgccatc
     3901 aaacgtcgag agccgcacgt gcctgtcttg tggcaacacc aaacctggaa taagaacgtt
     3961 gtagaggttc gtgcgctgaa agctgtagcg aagaatggcg tttttcgtgt gtttcttatc
     4021 cagagctttt tccacctgga acgaaacctg gccaagttcc gccgtgtaga tcggattctg
     4081 cgtgtttcgt tcgccggaaa cgccgaatag tgaactccat gtcgagccat gtaagtgtgg
     4141 atcggcgtag ttgaaaaaac cgcgctgatc caaccgtgag aggacggttc cgatcgtcgc
     4201 tgtttcggcc ctgcctcgca ggttgtgacg cgcaaaagcg aagctaaagc gcggccccac
     4261 ataactcttc tggctgactg taaacttatt cccaagactg atgggcggga tccccggaac
     4321 cgcgaccgag ttgacgggaa tattgccgga tcgagggatg atttcaagtc cgccgccaat
     4381 atccatcgaa ttgagcggcg actcgtggac cttaaccagc acttcttctt ggctttgatt
     4441 tacgaccggc cgcagcggct tgacgcttgt ccaatcgaaa attcccaagt cgtacaggtc
     4501 actttccgat tgcaggaaat gaccttcgct caaaggctga cctggtttca attgcgcgaa
     4561 agtaatctct ttgatgaagg atactttcgt atgcttcgct ccaagaaaga caacatcgct
     4621 gacgtgcgcc tgtgtgcctt cctcaattgt gtaggttacg tcgactgtgt gcggctggcc
     4681 cggcgccctc gaagcggccg atcggaccgt ggcatctgga tacccgagat caagataagc
     4741 cgctagcagc cgagtacggt ctgcctccat tagctttcgc gagaaagggt gtctgggctg
     4801 cagttgcaac gcatattttc gtcccagcgc tggcagagat tgcgttttgt tgccaacgac
     4861 gtgaagcgcg ccaaccttgt cttgcgggcc ttctgttatt tcaaaggtaa cggaaatctg
     4921 aggctcgaaa tcctccacct ttggcttgat cgagacgttt tcgaatccct gatctttgta
     4981 gagttgagtc agcgagttca cactcttgct caacagctgt tcactgtatc gtccatgcaa
     5041 gaagagaaat cctttcttga tggaaacatt cgatttcagc tcatcgtcgg agaaatagtg
     5101 gttgccgtcg aaatagacgc cggcttcgtg atgcttcgcg ccgcgttcca ctttgtaaat
     5161 gacttctagt ttttcgctcg ctctattcac attcgcgtcg accttggcgt cgaaataccc
     5221 cttggtctga aagtaggctc tcagattccg ctgaccctcg tcgatcaagt cctgatctgc
     5281 agcgccctct tcataaattg gaatcaaacg gtggagggtt tttgaggaga cccgcgcgcc
     5341 gctgactcgc acggacacct ccgggccagg tgtcacatcc agtctcaagt cgacccgatt
     5401 cgaatccgga acgtaggtcg aggaagccag ccgcaccgaa ggcgcgagtc ggttttggct
     5461 cctcaggcgg tcacggataa agtcgattga cctggcgatg cgcggctcgg aatatttttg
     5521 gccaggcttg agagttaccc gtttaagtct cgcccagatg ccctttaggg cagctttcaa
     5581 ctgttcgctc tgctgctcgc tgattccagt gaactcgatt ttgcggatgc gtgcctgttt
     5641 ccttaggtcg cagtggaaaa tcacattgac gactcgatgg gtctcatcat ggctcgtctc
     5701 tacttggacg tcggcagaga aatatcctcg cttgtgaagg taagcgagca agccctgttg
     5761 tcccttgact ccgaggccgg agaagtatgg cgactgttcg ggaatatcga ccgcttggag
     5821 aagagcggtg tacgggaact tggttccagt gccgggaaag tcgatcacgc ccacataatc
     5881 agtcggctgt aggataaata tcacttccaa tcccgcttgc tcgggttcca ggcttacttg
     5941 tatctcggag aactgacgcg tctgccgcag tgctgcgacg ctctcgttca gctctcgggt
     6001 cgaaaagggt tttccgggtc gcacttcaat ccgttcgcgc atcgctgtta tatccacgtt
     6061 ctggcgagcc gagatctcca ccgttgagac agtctggccg tcgaagcccg cataactcat
     6121 tggctgggtc tgctgggcaa aaagtaaacc gcctccaaga agatccagaa tcgggcaaag
     6181 ccagaaaagc gcgtaccggg cttggcgggc gcaatgccat agggaaacgt caacgagtcg
     6241 cctcttcacc taacagtcgc tccgttccgg gccttctcca tttcgtcgtc ggtctccgca
     6301 ccaaccagga caatcaaaca agcgctgtaa atccacaaca tcaatatgat gaaaccgccg
     6361 agggctccgt atactcgggg atacaatgag aaatgtcgga agtagatatt gaaccccgcg
     6421 gacagggcca ttagcgtaag cactgcaaag agcgtccccg gggtcagcca acgccaccgg
     6481 cgtcgcacgt ccggcagcag gaaattgagc aaatcgattc ccaaaaacat aagcgcgagg
     6541 gtcgcaatcc accgcccgat ttcccagacg gctcgcgata ccatcggagg ccccatgttg
     6601 accgcgagta ttttcgattc ccattgtccg aaagccgcaa ggccgaatgc tccgaggcca
     6661 aacatcgcag ccaggattgt aatcaccgtg gcgatagcat gtttgcgcca gtaagaccga
     6721 gaatccctaa caccatacgc aatgctgagc gactccatga ggctcacaaa tcccgccgaa
     6781 gcgctccaca gtgtcattac gaatccgaat gagaggaatc cagcggaata gttgaccaga
     6841 cccaaaatgg ttgaaaatac cagttcctgc gaactgcggg gtagataagt tacaacccag
     6901 gtcacaaatg ttttccacag cgcgctgtag ggcaaccacc cgacgatcac cgctaaaaca
     6961 agacaaaagg gaaaaaaaga aagtataaag taaaatgacg tttgcgcggc gagatcgaag
     7021 caatcatcaa gttgcaatcg attccaaatg cgctttagca cggcggacga tcgcagcagg
     7081 tatcgagaca cgcactccga tagtctatgc ggctgttctg ggcatcgtat aggggtaaac
     7141 ccttataacc acgataattt tcttgtaatt gggacctcgc caaattagag gcacagctca
     7201 gcccgtgcgt cacatatcgt cttcaattca cttttgcgcg gtagcttacg ggagagattc
     7261 taagagattt ccagtctgag aaaccatcaa tggttctgcg ctcgctctta ccggaacgca
     7321 cacgtttaga ttgcgagaga gcgaaatttc tagtatcaac gtggcgggct gcgacatgtt
     7381 gaacacgtca aggactgaag agaaatcgtt catgaaacga ctagccacgc tttcggcatc
     7441 ggtcgcgaca gccgtcctgg ctttagtttt tctctctcct cgatcaatcg cgcaggggcg
     7501 ggtcgcggtg gcgaagccgc ccggcgacgc tgtctatcgg aagagctgcg cgaagtgcca
     7561 ctcaagtgcc gctgcttcga accgggctcc tgatcttaag gcactgatga agctatctcc
     7621 cgagacgatt tacagcgcga ttaccacggg cgcgatggca actgcggcgc agcagctcag
     7681 tccaagcgac aagcaggtcc tcgccacata cttagggggg cgcccgcttg atctgacggg
     7741 cgcgggcagc gcagaacaca tgcgcaacca ttgcgaggcc aatgcgtcct tcgaaaacat
     7801 ttcatcaagg ccagcttgga acggctgggg cgtcaacttg gcaaacaccc gatttgaaga
     7861 tgcccaaggc gccgcgatga cggcgcagca agtgccgcac ttgaaattga aatgggcgtt
     7921 cggatttcct aatggtgaca catcgtttgg ccaaccgacg attgtaggcg ggcgcgtttt
     7981 tgtggggagc gatatcggat acttttacgc gctgaatgcc gagacagggt gcgtgtactg
     8041 gtcctatcgg gcgcaggcca ctgtgcgaac ggcggcggcc gttggtccga ttgccgatca
     8101 cggcacaacc aagtacgcgg tctacttcgg tgacttccgc gggaatgttt acgcgctcga
     8161 tgcttcgacc ggcaaggcgc tatggattaa gaacgtgatc gacaacaact acgttgcccg
     8221 cctcagcgcc gggcccacgc tgtataaggg ccgcctctac cttccgattt cctccactga
     8281 agaagtcttt gccgccgatc cgtcttatgc gtgttgcacg ttccgcggca gcatcgtcgc
     8341 acttgatgcg aatactgggc agacggtttg gaattcgtac acgatcccag aggcacccaa
     8401 gcccgtcagg aaaaatgtac gcggagtaca actctatgcg cccgctggcg gcgcgctgtg
     8461 ggattcccca acaattgatg cggagcgaca attgctctac gtcggtaccg gcgacgccta
     8521 tacggagcca gcgccgaaga cgacagatgc catcgtcgca ctcgatctga aaaccggcag
     8581 actcgtgtgg tcgtttcagg ccgttaagga cgacgcgtgg attctcgcgt gcgttcccac
     8641 gccgaccgac aattgtccga agaatctagg tcctgaccac gatatcggcg catcgccgat
     8701 tctggtgaaa atgcctgatg gtcatcggct actcatcgcc ttgcctaaga gcggaacaat
     8761 ctttgcgctc gatcccgacc gcaaaggcgc actggtttgg aagctggcta cgacgcaaac
     8821 cgtagctccg aataatggac aattggcgct gggcggcgcg agtgacggtc aaaatctgta
     8881 tgtggctctg gaagatggca cagctgtggc ggttgatctc gcaagcggca aacgtaagtg
     8941 gctcgctcgc ctcgaatcgt tggacgaact gggcgagcct acctccaacg gcgagaatcg
     9001 caccaaggcc ggcttgcgct tcggacaatc cgctgccgtt acggcaatac ccggcgtcgt
     9061 ttttaccggc ggctgggatg gaatcttgag ggcgctgtcg gcatcggatg gccgcctgct
     9121 ttggcaatac aataccgccc atgcgttcaa ggccgtgaat ggtattccgg caaaaggcgg
     9181 ctcgatgggt ggtcccggac ccaccgtggt caacggtgtt ctgtatgttc cttctggtta
     9241 tgctgcgttt ggcgggggaa tgcctggcaa tgttttactg gcactttcgc ctggagaatg
     9301 atcggtccgc gtgcatttcg gaatttccca gcacgcggaa acgaatctgc gagaagggta
     9361 ggctcatggg tcttgtaaat ttcggtcagc ccgacaatgg gattatccag acggcctaca
     9421 ttgttaagga tgtccgcgag gcgatcaatc gttgggtgaa agatctgaag gtcgggccat
     9481 ggttcttgct tgaacacttc acgggcgaag aggcgaaata tcgaggcggg ccctcaagtg
     9541 ccgacgttgc tatcgccatg agcttcgccg gccacatgat gatcgaactt atcgccccga
     9601 acgatgacgc gccatcagtc tatcgcgaga caatcgagaa acggggttac ggatttcatc
     9661 attggggcgt cgctacgtcg aatttcgatc gcgaggtcga acaacaccgt gcgcaagggc
     9721 atgaactggc ctttttcgcc cgtgtcccca gcggaggtcg cgtcgcatac atggatacga
     9781 ccgccgacct ggcgggcatg ctcgagttga tcgaactggg cgcttcgttc gacccgatct
     9841 ttagccgatt ctacggggcc acgattggct gggacggcaa acatccgata cgttcatttc
     9901 tctaggtaca tccggattgc cgccggccta ttcgtcgcga gtaatcaaga gctgcttgta
     9961 ggcctcccga cgtgtcaggc cgcgttcgcg ggccgcctgc tttagcgcgg cttttcggtc
    10021 taagccccgc tctcgcatga tgtcttcgac atggcgtgcc agcggaaccg tatcggcagg
    10081 ggcttgctca ttcccggtgt gttgcgcctg cccatctggc ggtgcgatga gcagcgtaat
    10141 ctcgccacgc ggagatttgg tccttgcggc ccggacaaga tcctcgagat gtccgcgcga
    10201 gaactcctcg aaggctttgg tgatttcccg ggccatcacg gcgggccgat tgccgaagat
    10261 ttcgagagcg tcctcgaggg tatcgagcaa acgatggggc gcctcgtata gcgcgagcgt
    10321 tcgcggctcg gtcgccagtt ctcgaagggc tttgcgacgc tcggtgggac gcgaaggcag
    10381 aaatcccgcg aacgtaaatt cctccgt
//
LOCUS       DATNDT010000293         2976 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_881305, whole genome shotgun sequence.
ACCESSION   DATNDT010000293 DATNDT010000000
VERSION     DATNDT010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2976
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881305"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VN788_09425"
     CDS             <1..270
                     /locus_tag="VN788_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HXU20788.1"
                     /translation="LPVIGVPMATSTLNGLDALLATVQMPAGIPVATMAIDKAGAANA
                     AIFAAEILALSDSEVAKKLVAHKEDLAKSVAEKNARLQKQLSQKQ"
     gene            complement(312..1334)
                     /locus_tag="VN788_09430"
     CDS             complement(312..1334)
                     /locus_tag="VN788_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20789.1"
                     /translation="MFGWHIRRSAALRASFAALLAVILAQTASRGSLQDEPGTLTEEM
                     VANLAAGRVIIAVVKDAILIATIENPIEAETRPPTPVVLSSERAGIILGADQWSSPSS
                     KQEIAQLDTELPHLRSHLLPSDPHLQAEQGGGEATDVEAVGQGLLERLNDVAQNLHAK
                     VNLSANEPLTELIVVGYLPGYGPDIWQLSYQIEQEEQELDYWTTRVLRPSYLQFWPPE
                     KGQPRTLLEFSYPSDHAPTPLLELLRQKDPRVEQILSSDPKMAEVADRFLQGESNKVL
                     AADATQFLRAILSVIAPPNARETMCSIREEAGLEWILAPPPESPAPKSHSERPPGAPS
                     LMGAPK"
     gene            1508..2086
                     /locus_tag="VN788_09435"
     CDS             1508..2086
                     /locus_tag="VN788_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HXU20790.1"
                     /translation="MSIFGALKGGHLRSLDSIRHGGKHVSDILAAHALFIRGDEKGVR
                     ADLSGADLSRADFAKAKLNYVNFQGANLKGASLREARLASAELNNCNLQAADLRKADF
                     TEANLSGADLSDAQCGGVEFFRANLQDAILRKADFGAANFREADLHGVDFEGANLKTA
                     ILRETKLEGVDLSRVVNLDSALLPRGYNRPDG"
     gene            2138..>2976
                     /locus_tag="VN788_09440"
     CDS             2138..>2976
                     /locus_tag="VN788_09440"
                     /inference="COORDINATES: protein motif:HMM:NF022457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo hydroxylase family protein"
                     /protein_id="HXU20791.1"
                     /translation="MPDFPWISVDDFRQPSGWVRPEAGQARARAYCEMLERGEILFFS
                     EPPFKLPAEDRQFLLAQQWAELRMHKNVSYRPGEDVLRGVSGAGANVDRLQAILRNYS
                     AEVIAFLQNFLAPYARDWIIDFSSFRPFEEESRGLPLHKRNDLLHVDAFPSRPTRGGR
                     ILRVFTNLNPAKPRVWNTTERFELLARQYAEDAGLQRIAADSWLSRTVQDWGGKLGFR
                     GMGRTPYDMFMLGFHDYLKENTAFQKECPKIRMEFPPFSTWLVFTDGVAHAAMSGQYA
                     IEQT"
BASE COUNT          628 a          821 c          893 g          634 t
ORIGIN      
        1 ctgcctgtga tcggtgttcc catggcaacc agcacgctaa acggcctcga tgcgctgctc
       61 gcgacagtgc aaatgccggc cggaattccc gtggccacca tggcgatcga caaagccgga
      121 gcggcaaacg cagctatctt cgccgccgag atactggcct tatcggattc cgaagtcgca
      181 aagaagctgg tggcccacaa agaagatttg gcgaaatcag tggcagaaaa gaatgcccgc
      241 ttgcagaagc aattgtcgca gaagcagtaa cccgcagctc aatcgaagga atcccgcgat
      301 ccttaagccg attatttggg cgcccccatt agtgagggtg cgccgggcgg gcgctccgaa
      361 tgacttttcg gcgcaggcga ttcgggaggc ggcgcgagaa tccattcaag cccggcctcc
      421 tcacgaatgc tgcacatggt ttcgcgagca ttcggcggcg cgattaccga caatatcgcg
      481 cggagaaatt gagtggcgtc agccgcaagc actttgttgc tttcgccttg tagaaagcgg
      541 tcggcgactt ccgccatctt gggatccgag ctgagaattt gttcgacgcg cggatctttc
      601 tggcggagga gttcaagcag cggcgtgggc gcgtggtcgc tcggataaga aaactccagc
      661 agcgttcgcg gctgtccttt ttccggcggc caaaattgca gatacgacgg ccttagaaca
      721 cgcgttgtcc aataatccaa ctcttgttct tcttgctcga tttgatagga gagttgccat
      781 atgtcaggtc cgtagcccgg caggtaaccg actacaatca attccgtgag cggctcattc
      841 gcggaaagat tcaccttggc atgaagattc tgcgccacgt cattgaggcg ttcgagcaag
      901 ccctgaccga ccgcttcaac gtcagtcgct tcgccgccgc cttgctctgc ctgcaggtgc
      961 gggtcggacg gcaatagatg gctgcgaagg tgaggcaact ccgtatccaa ctgcgcgatt
     1021 tcttgtttcg acgagggaga cgaccactgg tccgccccca gaattattcc ggcgcgctcg
     1081 ctggaaagga ccaccggagt gggcggccgc gtttctgctt caatgggatt ttcgatcgta
     1141 gcgattaaaa ttgcgtcttt gacgaccgca attatcacgc gcccggcagc cagattcgca
     1201 accatctcct cggtgagcgt tccgggctca tcctgcaacg agcctcggga agcggtttgc
     1261 gcgaggataa ccgccagcag cgccgcaaat gacgcgcgaa gggccgcact ccgccgaatg
     1321 tgccacccga acacgagcga gccttccttg ggaactgccg cgacactcta cctaatcgag
     1381 ttcacggcaa ttctcatccg accaaagtta ccacaatctg catcaagcgg attcattcgg
     1441 atcggtaggc aggcggctgc gtctcttgta aactagggcg atagctaatc tgattcgcgg
     1501 gtgaaccatg agcattttcg gcgcactaaa aggagggcat ctgcgatcgc tcgatagcat
     1561 tcgccatggt ggtaagcacg tttccgatat tctggccgcg cacgccctgt ttatccgggg
     1621 cgacgaaaaa ggtgttcgcg ccgatctgag tggtgctgat ctttcgcgag ccgatttcgc
     1681 aaaggccaaa ctcaattacg tgaattttca gggagcgaac ctcaaaggcg cgagtttgag
     1741 ggaggcgcgc ctggcgtcgg cggagctgaa caattgcaat ctccaggcag cagacttgcg
     1801 caaggccgat ttcaccgaag cgaacttgag tggcgctgac ctgagcgatg cgcagtgcgg
     1861 cggagtggaa ttcttccgcg cgaatttgca ggacgcgatt ttgcggaagg cggacttcgg
     1921 agccgccaat tttcgcgagg cggaccttca cggggtagac ttcgagggcg ccaatctcaa
     1981 gacggcgatt ctgcgggaaa cgaagctcga aggcgtggat ttgtcccgcg tagtaaatct
     2041 ggattcagcg ctgttgccgc gcgggtataa ccggccggac ggctaaacca acggggagtc
     2101 tttcgaaacg ttcgtttcgc gtaattgagt tatgaagatg ccggattttc cttggatttc
     2161 cgtggatgat ttcaggcagc cgtcgggctg ggtgcgccca gaagcgggcc aagcgagggc
     2221 acgagcatat tgcgagatgc tcgagcgtgg cgaaatcctc tttttcagcg agcctccttt
     2281 taagcttccc gcagaggacc gccagttttt gctcgcgcaa cagtgggcgg agcttcggat
     2341 gcacaaaaac gtatcttacc ggcccggcga agatgtcttg cgaggtgttt ccggggctgg
     2401 ggccaacgtc gaccggctgc aggcgatttt gcggaactac tcggcggaag tgatcgcgtt
     2461 tctccagaat tttctcgcgc catacgctcg agactggatc atcgacttct ccagttttcg
     2521 cccgtttgag gaagaaagcc gcgggttgcc gctgcacaag cgcaacgacc tgcttcatgt
     2581 ggatgctttt ccaagccggc caacgcgcgg cggccgcatt ctgcgggtat tcacgaatct
     2641 aaatcctgca aagccgcgtg tgtggaacac taccgagaga ttcgaacttc tcgcccggca
     2701 gtatgcggag gacgccgggt tgcagcggat cgccgcagac tcctggctga gccgaacggt
     2761 gcaagactgg ggcggaaagc tcggttttcg tggaatgggc cgcacccctt acgacatgtt
     2821 catgctgggt tttcacgatt acctgaagga gaacaccgcg tttcagaagg agtgtcccaa
     2881 gattcgaatg gagtttccgc cgttttccac gtggcttgta tttaccgacg gcgtcgcgca
     2941 cgcggcgatg tcgggacagt atgcaattga gcaaac
//
LOCUS       DATNDT010000294         3898 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1772366, whole genome shotgun sequence.
ACCESSION   DATNDT010000294 DATNDT010000000
VERSION     DATNDT010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3898
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1772366"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /locus_tag="VN788_09445"
     CDS             complement(<1..440)
                     /locus_tag="VN788_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018199086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU20792.1"
                     /translation="MPNEYASGESAVISVRNLRVSYGTHEILHGINFEVQPGETLVIL
                     GGSGSGKSTLLRTLVGLEQPSGGEIWIRGRNFANLPEAERDDLRKKMGMSFQGGALFG
                     SMTVGENVALPLREHTQLDESTIEIMVRLKLDQVGLSGFEDYMPA"
     gene            complement(465..1262)
                     /locus_tag="VN788_09450"
     CDS             complement(465..1262)
                     /locus_tag="VN788_09450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20793.1"
                     /translation="MPFVETIGEATIDGLAYVGGLARLAGGAARAVFLESFFGRKLRW
                     YRAVHQAMAIGVEAIPIVSLISFFVGMILALQGAYELRTLGAMQLVASAVAISMTREL
                     GPLMTAIVVIGRSGSAFAAEIGTMKVNEEIDALETMALEPVRFLVAPKFLAMLLMMPC
                     LTTWADFMGILGGGLFGVTNAGFTWATYLHATIDAMALRDVVTGLIKSVMFGLVITAV
                     GCHEGFSTGLGSEQVGRSTTSAVVKSIFLVIAVDLVFTSIFYFTAPR"
     gene            complement(1284..1643)
                     /locus_tag="VN788_09455"
     CDS             complement(1284..1643)
                     /locus_tag="VN788_09455"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU20794.1"
                     /translation="MNISTRKLENATIVDVIGDITLYNSPEVRKSLLGTLRDHSVSRV
                     IVNLGKVNYIDSAGIASLVEGLKVSRDLKKGFALYGLSSTAREVLQLTRLLKVFEVHD
                     TEKEALGAGSGGAAVSQ"
     gene            complement(1788..3128)
                     /locus_tag="VN788_09460"
     CDS             complement(1788..3128)
                     /locus_tag="VN788_09460"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011745897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HXU20795.1"
                     /translation="MNPTKSEPALNTETSATTRRDTVTLIDNRNGRRYDVPIKNDTIR
                     AIDLRPIKISESDFGMMSYDPSFSNTASCTSKITYIDGDNGILRYRGYSIEELAEKST
                     YPETSYLILHGELPTKSQLADWNYHITHHTFIHESIKKFLDGFHYDAHPMGMLISTLA
                     ALSTFYPDAKDIFSAESRLKQTYRLIGKMPTLAAFAYRHSLGMPFVYPDNDLSYCGNF
                     LNMLFRTTELQYRPNPTLERALDTLFILHADHEQNCSSTAMRGVGSSHVDPYSAAAAA
                     TAALYGPLHGGANEAVLRMLLEIGSVNNVPAYVKSVKAGEKLLMGFGHRVYKNYDPRA
                     KIVKRIAYEVFDIMGRNPLIDIALECERIALEDEYFVKRRLYPNVDFYTGLIYQSMGF
                     PVTMFPVLFAIPRTSGWIAQWEEMLLDPEQKISRPRQVYAGYDLRSYIPTDHRG"
     gene            3531..>3898
                     /locus_tag="VN788_09465"
     CDS             3531..>3898
                     /locus_tag="VN788_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HXU20796.1"
                     /translation="MSAPVSLPQIMAHRQTGLEVWMDRVLETAPKAEPNWRKHEVHDL
                     RVALRRCRNMCEALSEVSPGSRWRRIKKVSRKLFHASGDLHDTQVERDWVKKCGPAGD
                     PVRKYMLRLLSHQEKKQRKTA"
BASE COUNT          846 a         1154 c         1080 g          818 t
ORIGIN      
        1 gcaggcatgt agtcttcgaa gcccgaaagt cccacttggt cgagctttaa gcgcaccatt
       61 atttcgatgg tggactcatc gagctgtgta tgctcgcgca agggaagggc gacattctct
      121 cccacggtca tcgacccgaa tagcgctccg ccctggaacg acattcccat cttctttctc
      181 agatcgtcgc gctcggcttc cggcaggttc gcgaaatttc ttccacgaat ccagatctcg
      241 ccgccgctcg gctgttcgag tcccacaagc gtacgaagga gcgtgctctt acctgagccc
      301 gagccgccta gaatcaccag tgtctcgccg ggctgtactt cgaagttgat gccgtgcaga
      361 atttcgtggg tgccgtaact gacgcgaaga ttccgaaccg aaatgaccgc cgattcgccg
      421 gacgcgtatt cgttgggcat aatctcggcc ccaccgttct gtggctatcg aggcgccgtg
      481 aaataaaaga tgctggtgaa aaccaaatct accgcgataa ccagaaaaat agacttcacc
      541 accgccgaag tcgtcgagcg gccaacttgt tccgagccga ggcccgtaga aaacccttca
      601 tggcagccca cggcggtgat caccaggccg aacatcacgc tcttgatcag gccggtgacc
      661 acatcgcgca gggccatggc atcgatcgtg gcatggaggt acgtggccca cgtgaatccc
      721 gcgttggtaa cgccgaagag gccgccgccc aagatgccca tgaaatccgc ccatgttgtg
      781 aggcagggca tcatcagcag catggccagg aatttcggcg ccaccagaaa gcgaaccggt
      841 tcgagcgcca tggtctccag cgcgtcgatt tcctcgttca ccttcatcgt gccgatttcc
      901 gcagcgaaag cggagccgga acggccaatc accacgatcg cggtcatcag cggccccaac
      961 tcacgcgtca tcgaaatggc cacggcgctc gccaccaact gcattgcgcc gagggtccgc
     1021 aattcgtagg ctccttgcag cgccaggatc atgcccacga aaaacgaaat gagagagacg
     1081 atgggaatcg cctctactcc gatggccatg gcctgatgca ctgcccggta ccatcgcagc
     1141 tttcgtccaa aaaaggactc aaggaataca gctcgcgccg cgccgccggc caggcgcgcc
     1201 aatcccccga cgtaagccaa tccatcgatt gtggcttcgc cgatcgtctc tacgaatggc
     1261 atctggaggc cgggatattc gcgctactgg gacacggcag caccgccgct tcccgcgccc
     1321 aaagcttcct tttctgtatc gtggacttca aaaactttca gcaggcgggt aagctgaaga
     1381 acttcgcgcg ccgtcgagct caggccatag agagcgaacc ccttcttcag gtcccgcgag
     1441 accttcaggc cctcgacaag cgaggcgatt ccggcgctgt cgatatagtt cacctttcca
     1501 aggttcacga tcacgcggct cacgctatgg tccctcagcg tgccgagcag gctcttgcgt
     1561 acttcaggag aattgtaaag tgtgatgtct ccgatcacgt ccacgatggt ggcgttctcg
     1621 agcttgcgtg ttgagatatt cattagcgca gcctgcgcgg cgtcccagtg ttttgccaga
     1681 gatcgtgctc gaatgcaatg cattttaggg gcgctgcagc agcgaacgcc gcgcgaagga
     1741 aggttgatcg gaaataaatt tcgccgggcc gaacctcgaa ataagctcta gccgcggtga
     1801 tcggtcggaa tatagctgcg aagatcgtag ccggcgtaaa cctgccgcgg ccgggaaatc
     1861 ttttgctcgg gatcgagcag catctcttcc cactgtgcga tccatcccga agttcgcggg
     1921 atcgcgaaca gcaccggaaa catggtcacc gggaagccca tcgattggta gatcaatccc
     1981 gtataaaaat ctacgttggg atacaagcgg cgtttgacga aatattcgtc ctcgagggcg
     2041 atcctctcgc attccagagc aatatcgatc agcgggttgc ggcccatgat gtcgaacact
     2101 tcgtacgcga tgcgcttcac aattttggcg cgcgggtcgt aattcttgta aacgcgatgg
     2161 ccgaatccca tcagcaattt ctcgcccgcc ttcacgctct tcacgtatgc gggtacattg
     2221 ttcaccgaac cgatctccag cagcatgcgc aacaccgctt cgttcgcgcc tccgtgcagc
     2281 gggccataca gcgccgctgt ggctgcggcc gccgcggaat acgggtccac gtgcgagctg
     2341 cccacgccgc gcatcgcggt ggaagagcaa ttctgttcat ggtccgcatg caagatgaaa
     2401 agagtatcga gcgctcgctc gagcgtcgga ttgggacggt attgcagttc cgtggtgcgg
     2461 aacagcatgt tcaggaaatt gccgcagtag ctcagatcgt tgtcgggata aacgaagggc
     2521 atgcccaagc tgtgccggta ggcaaacgcc gccaacgtcg gcatcttgcc gattagccga
     2581 taggtttgct tgagcctcga ttcagcgctg aaaatatcct tcgcgtcggg ataaaaagtt
     2641 gagagcgccg ccaaagtcga gatcagcatc cccatcgggt gagcgtcgta gtgaaaacca
     2701 tctagaaact tcttgatgct ctcgtggatg aacgtatggt gggtgatgtg atagttccaa
     2761 tcggccagct gcgatttcgt gggcagttcg ccgtggagaa tcaagtacga cgtttcagga
     2821 taagtgcttt tttccgccag ctcttcgatc gaatagccgc ggtagcgcag gatgccgttg
     2881 tcgccgtcga tgtacgtaat cttgctcgtg caggacgccg tgttcgaaaa cgacggatcg
     2941 tagctcatca ttccaaaatc ggattcgctg attttaatcg gcctcaaatc gattgcccgg
     3001 atcgtatcgt tcttgatcgg cacgtcatat cgccggccgt tgcggttatc gatgagggtt
     3061 accgtgtccc ggcgcgtagt cgctgaagtc tcagtgttaa gcgccggttc tgatttcgtc
     3121 ggattcattc cctaattcct tccaagaggg agcaggccgc tggcaccact aaagcgcagc
     3181 atcaacaatg tacaccaaaa cccgcgtctg acgaaccgct ttgcatcgcc agcgattcag
     3241 catgaagccg gctctgcggc ttcatccgga gcggtctgtg cgtcacgcgc ccaaaaagcc
     3301 gattctccac accacaccgt ggacgacttt tctttacacc aacaactact tgcgcgattc
     3361 gaccccctcg tgtaaagatt ttatccgtat cctcgtagtg cgtccaattc ggcccgccgc
     3421 gctccagcac gcacgccgcg gcattgctcg tgcccccgct ggcaaaaggc ctcgtcgggc
     3481 ggtaatataa aaacgtcgtt tttagcctgc cggatagatc ggattttcgc gtgtcggctc
     3541 ccgtctcatt gcctcaaatc atggcgcatc ggcaaaccgg tcttgaagtg tggatggacc
     3601 gcgtgctgga aacggcgccc aaggccgagc caaactggcg gaagcacgaa gtccatgatt
     3661 tgcgcgttgc tctgcggcgc tgccgcaaca tgtgtgaagc tctcagcgaa gtgagccctg
     3721 gctcccgctg gcgcaggatt aaaaaagtca gccgcaaatt atttcacgct tcgggcgatt
     3781 tgcacgatac gcaggtggaa agggactggg tgaagaagtg cggccccgcc ggcgaccccg
     3841 ttcgcaaata catgctgagg ctgctctccc accaggaaaa gaagcagcgc aagaccgc
//
LOCUS       DATNDT010000295         3855 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2899485, whole genome shotgun sequence.
ACCESSION   DATNDT010000295 DATNDT010000000
VERSION     DATNDT010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3855
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2899485"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /locus_tag="VN788_09470"
     CDS             complement(<1..1160)
                     /locus_tag="VN788_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HXU20797.1"
                     /translation="MTSTTVSAKGPFAGTPEFEKNLDRLAEVAVRAGLGLAPGQELVM
                     TATLDSAPLARLIIEHAYKAGASLVTTLFSDEQSALLRFRYAPDPSFDAAPAWLYEGM
                     AQAYRNGAARLAITGNNPSLLSKEDPEKVSRLNRATSKAYRPALELITRHEINWTIIA
                     CATPEWAAAVFPDLPQDEALARLWTAIFAASRADQPDPVAAWKKHDAHLHSRADMLNQ
                     KRYSALHFRGPGTDLRVGLADDHLWLGGGTTAGNGCYCIPNMPTEEVFTTPHKDRVDG
                     RVTSTKPLSHQGTMIEEISVRFEAGKIVEAHAARGEQVLQRMIETDEGARRLGEVSLV
                     PHSSPIAASGLLFMNTLFDENAACHIALGQAYSTCLKNGDTLTPEQLASKGA"
     gene            1371..1775
                     /locus_tag="VN788_09475"
     CDS             1371..1775
                     /locus_tag="VN788_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20798.1"
                     /translation="MNRRQLLSLLGMSPALLAASPAEAERKKKDAGSTAGSEAKLTAV
                     SPKGTPPPIQLYSMAPRASSLDGKTIYVVDATSFLGSATLLHNMQAWFQKNMPNVKTV
                     FRTKAGQYAADDPKLWAEIKANAYACVMAIGH"
     gene            1797..3143
                     /locus_tag="VN788_09480"
     CDS             1797..3143
                     /locus_tag="VN788_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXU20799.1"
                     /translation="MSHCVTVEKMGVPAIPMCTQAYTELAKLTASRDGMPHLRQVFTP
                     HPVGGKTDAELAAYLVNPDPVSGIQIMKEIVDDLTRPLTADEQKTGMQAIAAGPATFG
                     PDTVDNLQEIFMNNGMTDYMPIIVPTEEKVQAMLKATSHKPDEVLRTPVQGVGYTSAR
                     WGYTVRQVAVNAVMSGCRPEYFPIVLAMASLGPLGLFGSTTSSSTAIVINGPIRDKLN
                     MNYGLGALSPFAQANATIGRSWTLMGKNLTNGGIPGDTYQGGQGNQLDYNNCVIAEDE
                     KNSPWTPLHVQFGFKPEENVVSIFGGPDVRQGHGARGAGAEIAMFDQQISFMCQSLVG
                     GSGALAILDPLVAKRLVDQGYDNKEKLSEWVFKNTQRTVKDFKESFLASFHLYPLALQ
                     GREPYASWYKLPDDAMIPFFVSAKQINFVVCGGQANAFFQIANMNHGRSASIDKWM"
     gene            complement(3616..>3855)
                     /locus_tag="VN788_09485"
     CDS             complement(3616..>3855)
                     /locus_tag="VN788_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20800.1"
                     /translation="QPRSKTQALKQAKFDGSVESSLVQQGFDGKAKQNRGFGMPLLRK
                     QESPQVSSPCGCGTHPFAISPASSVPLHGLRSPIL"
BASE COUNT          808 a         1005 c         1246 g          796 t
ORIGIN      
        1 tttgcacctt tcgatgcgag ttgttctgga gtgagggtgt cgccattttt caggcaggtg
       61 ctgtacgcct gtccgagggc gatgtggcag gcggcgtttt cgtcgaagag ggtgttcatg
      121 aagagcaagc cgctggcagc aatcggcgac gagtgcggga ctagagacac ttcgccgagg
      181 cggcgcgcgc cttcgtccgt ttcgatcatg cgctgcagga cttgttcgcc gcgcgcggcg
      241 tgggcctcga caattttgcc ggcttcgaat cgcacggaaa tttcttcgat catcgtgccc
      301 tgatgggaga gcggcttggt gctggtgacg cgcccatcga cgcgatcttt gtgcggcgtg
      361 gtgaaaactt cttcggtggg catattagga atgcaatagc agccgttgcc cgcggttgtg
      421 cctccgccga gccatagatg atcgtcggcc aggcccacgc ggagatcggt gcctggtccg
      481 cggaagtgca aggcggaata acgcttttgg ttcagcatgt cggcgcgaga gtgcaaatgc
      541 gcgtcgtgct tcttccatgc ggcgacggga tcgggctggt ccgcgcgcga tgcggcgaag
      601 atggcggtcc aaagacgggc aagggcttcg tcctgcggaa gatcggggaa cacggccgcg
      661 gcccactcgg gtgtggcgca ggctatgatg gtccaattga tttcgtggcg ggtgatcagc
      721 tcgagcgccg ggcgatatgc tttcgaagtg gcgcggttca gccggctcac cttttccgga
      781 tcctctttcg agagcagcga aggattgttg ccggtgattg ctaaacgcgc ggcgccattg
      841 cggtatgcct gagccatgcc ttcgtagagc cacgctggag cggcgtcgaa gctaggatct
      901 ggagcgtaac gaaaacgaag cagggccgac tgctcgtcag aaaacaaagt ggtgacgaga
      961 gaggcgcccg ctttgtatgc gtgctcgatg atgagtcgcg cgagaggggc tgagtcgagc
     1021 gtggcggtca ttaccagctc ttgtcccggc gcgagtccca gcccggcgcg gaccgcaact
     1081 tcggcgaggc ggtcgagatt tttttcgaat tcgggagtgc cggcaaaggg tcccttggcg
     1141 gatactgtag tcgaggtcat ggctagcatt atcgcgcgag gctcgcggat cgacaacgcg
     1201 cggaagttgg ctgaggcatt gcgttttccg gcgcgcagtt gtacgttgtc gggcggcatc
     1261 ggaggctata accaaaatcc gttacggtac gtctatctgg ataagggcag aagacggctg
     1321 gtctttgtcc tgcatttggg ttctgcattt ggaaaagggg gaatggaatc atgaacaggc
     1381 gccaattgct gtcgctgctc ggcatgtctc cggcacttct cgcggcgtcg ccagcggaag
     1441 cggaacggaa aaagaaggat gcgggaagca ccgcgggaag cgaagccaag ctgacggcgg
     1501 tgagtcccaa gggcactccg ccgcctatac agctctattc catggcacct cgagccagct
     1561 cgctcgacgg gaagacgatt tacgtggtgg acgccacgag ttttctcggc agcgcgacgc
     1621 tgttgcataa catgcaggcc tggtttcaga agaacatgcc caacgtgaag acggtcttcc
     1681 ggacgaaggc cgggcagtat gccgcggacg atccgaagct ctgggcggag atcaaggcaa
     1741 acgcgtacgc gtgtgtgatg gccattgggc attgaggagg ctgctcacca gcgaccgtga
     1801 gtcactgcgt aaccgtggaa aagatgggcg tccccgcaat tccgatgtgc actcaggcct
     1861 acacggaatt ggccaagctg actgcgtcac gagacggcat gccgcacttg cgccaggtgt
     1921 tcactcctca ccccgtgggg ggcaagacag acgcggaact cgcggcgtat ctggtgaatc
     1981 ccgacccggt atcgggaata cagatcatga aggagattgt tgacgacctg acccggccgc
     2041 ttacggcgga tgaacagaag accgggatgc aagccattgc ggctgggccg gcaacattcg
     2101 gtccggacac agtggataac ctgcaagaaa ttttcatgaa caatggaatg acggattaca
     2161 tgccgatcat tgttcccacg gaagaaaaag tacaagcgat gctgaaggcg acgagccaca
     2221 agccggacga ggtgctcagg acgccggtcc agggagtggg ctacacgtcc gcgcgatggg
     2281 gctacacggt gaggcaggtt gccgtgaacg cggtgatgtc gggctgcagg ccggagtatt
     2341 ttccgatagt gcttgcaatg gcatcattgg ggccgcttgg gcttttcggg tcgacaactt
     2401 cgtcgagtac ggcgatcgtg attaacggtc cgattcgcga caagctcaac atgaattacg
     2461 ggctcggcgc gctgagtcct ttcgcgcagg ccaatgccac aatcgggcgc tcgtggacgt
     2521 tgatgggaaa gaatctcacc aatggaggta tccccgggga tacctatcag ggcgggcagg
     2581 gcaatcaact cgattacaac aactgcgtga tcgcggagga cgagaagaac agcccatgga
     2641 cgccgcttca cgtgcagttc ggattcaaac ccgaagagaa cgtggtgagc atattcggag
     2701 ggccggatgt gaggcagggc catggcgcga ggggcgcagg agctgaaatt gcgatgttcg
     2761 atcaacagat cagcttcatg tgtcaatcgt tggttggcgg atcgggcgcg ctagcaatct
     2821 tggatccgct ggtggcgaag cgtttggtgg accagggata cgacaacaag gaaaagctat
     2881 cggaatgggt tttcaagaac acgcagcgca ccgtgaagga ttttaaggaa tccttcctgg
     2941 cttccttcca cttgtacccg ctcgcgctcc agggaagaga gccttatgcg tcatggtaca
     3001 aattgccgga cgatgcgatg atcccgttct tcgtctcggc gaaacagatt aacttcgtgg
     3061 tttgcggcgg gcaggccaac gctttctttc aaatcgccaa catgaatcac ggcaggagcg
     3121 cgtcgatcga caagtggatg taaacgccta aaggatgata gcagtcaagg atttgcgttg
     3181 aggcgaaccg aactcctagc aatgttctga agttttcgaa agttcggcac agtcatgaag
     3241 gtgaattcga ctcccatgcc tctggctgga agcgaccttc gaaccagtgc ccggctttgc
     3301 ggtccctctt cgatttctag agaaagctca atttcgtccc caaccgaaac ggtgttccgc
     3361 gtctcaatga agcaaccatt caggctgatg tcgctgagag tgccaattgc actcggcgcc
     3421 gagccggcaa caaagcgagc cggtattttg cagggaagac gactgaaaac tcgtctttcc
     3481 acttgattcg aatcggccgg gctctttgcc tccttgtctg gctgatcctg cggcggattc
     3541 ccgtttgagt gatcggccat cgccggcggc tgaagccgct gagtaatcgt tagttcgggg
     3601 cggctctcag cgtggctata aaatcggtga gcgcaatcca tgcagcggaa cggacgacgc
     3661 gggagaaatc gcgaaaggat gcgttccaca accgcacggc gatgagacct gcggactctc
     3721 ctgcttccgc aaaaggggca tgccaaatcc gcggttttgc ttggcttttc catcgaaacc
     3781 ttgctggacg aggctggatt cgacgctgcc gtcgaatttc gcctgcttca atgcctgggt
     3841 tttcgatcgc ggctg
//
LOCUS       DATNDT010000296         2915 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_907239, whole genome shotgun sequence.
ACCESSION   DATNDT010000296 DATNDT010000000
VERSION     DATNDT010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_907239"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            317..1006
                     /locus_tag="VN788_09490"
     CDS             317..1006
                     /locus_tag="VN788_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20801.1"
                     /translation="MQTNIKASHREAPVCGGQARKVVRNGSSMQWQRQFKNIRIQGEG
                     AMTAAKVIFLAILGVMIAACTTARAQTDQVPTAWGDAGDGLQIALYFDPSKRQDSGPD
                     NVKLALRNVGVAEMNVVLGAGCGQFSPTNKIALVVTDREGKSLRFPDLAGNWFCAGLP
                     GSFSVSLTPRAYYLMPFQISDYLKPDVMRAAVSAGVNSVQADLEIDRRGPGNATLVSH
                     VKSNQLKMTIL"
     gene            1162..1326
                     /locus_tag="VN788_09495"
     CDS             1162..1326
                     /locus_tag="VN788_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20802.1"
                     /translation="MADSTILGFSSQDFGEIYEAENPLAELLRKIVMIETRNAVDEAP
                     SVFEDWEAFG"
     gene            1423..>2915
                     /locus_tag="VN788_09500"
     CDS             1423..>2915
                     /locus_tag="VN788_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXU20803.1"
                     /translation="MPPEDEQLSRASSRPDIRHLLGIVTAVVVVAALYFARVVFIPVA
                     LAVLVAVVLTPAVSFLERLRLYRSVAIFVVMAALVCVAGLIGWTVLPQFVDLMAQLPK
                     YEQAIQYKVDRLKGKTTGKLSSATESVRSLEKGITRTNGPPSEGKNENAPAPGTSPER
                     PLAVEMVNPSSPLQAAKGLADPIATGIAVLVFAVFMLAGREDLRNRLIKLTSGGRLTV
                     MTQAMAEAWSRINRYLFMQMLVNSCYGLSIGVSLHFLGVPKAALFGFAAGVLRYLPYI
                     GWMIAAAMPTALALAVFPGWEHAIVTICIFVGLEMIVANAVEPLIYGAHVGMAPLAIL
                     IAAIFWTLIWGFPGLLLSTPVTVCLVVIGRYVPSLGFFSILLGNEPVMAPHAQFYQRL
                     LAGDQNEARQILENCLKEKTLEDLYDSVLIPALARSEQDGHRNELDEETRSFIDSTTR
                     ELAEEIVVSRTQGHEAGESVLELERSHQETNERPKIICVPARDEADEV"
BASE COUNT          631 a          730 c          900 g          654 t
ORIGIN      
        1 gatccggctg cgtataggga agcggattca gaaggacgcc gtcgacaacg gagaagagcg
       61 ccgtattcgc gccgatgccg agcgccaatg taacgatcgc gatgatggtg aagcccggcg
      121 ctttcgaaag gatacggacc gtgtaacgaa tatcttgcag caaagaattc acagcccacc
      181 tctggacgaa aagtatatgt ccacacccgt cgggtgcgag cgcttaacaa gagtacgcac
      241 ggaggaacgc aaaagttcgc tccgtccatg gcaagagtat gatggccgtg ttccacggtg
      301 gaggagcagg caatgaatgc aaaccaatat caaggcttct caccgagaag cgcccgtctg
      361 cggcgggcag gcgcgtaaag tcgtgcgcaa cggttcctct atgcagtggc aaagacaatt
      421 caagaacatt cgaatacaag gcgagggagc catgactgcg gccaaagtca tttttctggc
      481 gattctgggt gtgatgatcg ctgcgtgcac aacggcacgg gcgcaaacgg atcaggtgcc
      541 gaccgcgtgg ggagatgctg gtgatggtct gcagatcgcg ctctatttcg atccaagtaa
      601 gcggcaggat tcgggcccgg acaatgtgaa gctcgcttta cggaacgtgg gagtggccga
      661 aatgaatgtg gtgctcggcg ctgggtgtgg ccagttttct ccgacaaaca aaatcgccct
      721 ggttgtgacc gacagagaag gaaagtcgct ccgattcccg gatcttgccg ggaattggtt
      781 ttgtgcaggg ctacccggat cgttttccgt gtctctcacg ccccgcgcct attatttgat
      841 gccattccag attagcgact atttgaagcc ggacgtcatg agggcagcgg tgtcagccgg
      901 agtgaattcc gttcaggccg acctcgagat agatcgacgc ggtccaggca atgccactct
      961 cgtctcacat gtgaagtcaa atcaattgaa aatgacgatc ctctagatcg ctgaccattc
     1021 gttcggttat cttcgcattc cccctaccaa caaggtctga tccgtccctc atctcaatag
     1081 aacgcctgac attgctacag atttgcatcg attgctggtt ggccggcaga aaggtagggt
     1141 gcggaacaag ggaggcctga cgtggccgat tcgaccattc ttggattcag ttcgcaggat
     1201 tttggcgaaa tatacgaagc agagaatccc ctggctgagc tattgcggaa gatcgtgatg
     1261 atcgaaacga gaaatgcggt ggacgaggct ccgtcggtat ttgaagattg ggaggcattt
     1321 gggtaggagc tgcccaaaat ctccgggcgg aagcagcatt ttccgagggg gctgggcgtc
     1381 tctgtttaag tatcggtcgt cggaacgcag ccgaaagtag ctgtgccacc ggaggatgag
     1441 caactgagca gagcgtcgtc acgaccggat attcgtcacc tgctggggat cgtcaccgct
     1501 gtagttgttg ttgccgcgct ttatttcgca cgcgttgtct tcattccagt cgcactagcg
     1561 gtccttgtgg ccgtggtgtt gacgcccgcc gtttcatttc tagaacggtt gcgcttgtac
     1621 agatcagtgg cgatcttcgt tgtcatggcg gcgctggttt gcgtggccgg attgatcgga
     1681 tggactgtgc tgccgcagtt tgtcgatttg atggcgcaat tgccgaaata tgagcaggcg
     1741 attcagtaca aggtggatag gctcaaaggg aagaccaccg gaaaactcag cagcgcgaca
     1801 gagtccgtca ggtcgctcga aaaggggata acaagaacga acggcccgcc ttcagagggt
     1861 aaaaacgaaa acgctcctgc cccgggcacg tcgcctgagc gtccgcttgc ggtggaaatg
     1921 gtgaatccct caagtccgct tcaggccgca aaggggctgg cggatcccat agcgaccggc
     1981 attgctgtgc tggtatttgc cgtttttatg ctggccggcc gcgaggattt gcggaatcga
     2041 ctgatcaagc tcacttcagg cggcaggttg acggtgatga cccaggcgat ggccgaagcc
     2101 tggagccgca ttaaccgtta tctgttcatg cagatgctgg tgaactcgtg ctatgggcta
     2161 tcgattggtg tttcgcttca cttcctcggt gtgccaaagg cggcgctgtt tggattcgcc
     2221 gctggcgtgt tgcgctactt gccctacatc ggatggatga ttgctgctgc aatgccgact
     2281 gcgctggcgc ttgccgtttt tccgggatgg gaacacgcaa tcgtcacgat ttgcattttt
     2341 gtcggtttgg aaatgatagt cgcgaacgcc gtggaacccc tgatttacgg agcccatgtg
     2401 ggcatggcgc cattggcgat tctgattgcc gcgatctttt ggacgctcat ttggggcttc
     2461 cccgggctat tgctttccac gcctgtgacg gtttgcctgg tcgtgattgg ccgttacgtg
     2521 ccaagcctag gtttctttag cattctgttg gggaatgagc ccgtgatggc gcctcacgcg
     2581 cagttctatc agcggctgct tgcaggcgat cagaatgagg cgcggcaaat actggaaaac
     2641 tgcttgaaag agaagacgct ggaagatctc tacgactcag tgttgatccc tgccctggcg
     2701 cgctcggagc aagacggaca tcgcaacgag cttgatgaag agacgcgaag ttttatcgat
     2761 agcacgacgc gcgaacttgc tgaggagatc gtcgtgtcgc ggacgcaggg gcacgaggcg
     2821 ggcgaaagcg tcctggaact tgaacgttca caccaggaaa cgaatgagcg gccgaagata
     2881 atctgcgtcc cggcgcgcga cgaggcggat gaggt
//
LOCUS       DATNDT010000297         8158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2609868, whole genome shotgun sequence.
ACCESSION   DATNDT010000297 DATNDT010000000
VERSION     DATNDT010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8158
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609868"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..266
                     /locus_tag="VN788_09505"
     CDS             <1..266
                     /locus_tag="VN788_09505"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HXU20804.1"
                     /translation="RGREGMGMGDVKMMLMAGAFLGAKRTLLTIFAGSLLGSILGLAF
                     MAARSKDSNYELPFGTFLGMAALLVVFFGTPVVSWYQSILLGR"
     gene            289..1710
                     /locus_tag="VN788_09510"
     CDS             289..1710
                     /locus_tag="VN788_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU20805.1"
                     /translation="MNAIVPINHTAVPTAGLVYAAATLAILVVALLVLFIIARKFLRK
                     PGAAPEADWAKPKADMENASAFMAASMQGVIEKLRAQEKELARLHMLAQERAQESERL
                     TEEVTRHMPTGMLLVNATGAISSTNPAAEDALGRRPVRYRSYKEILGDDSGLSQMLTA
                     CLRDGKTFQRGEVEHLTNDGEVRRLGVTISPIYRPTRAAVRSRVPGATEQPEMKVSGA
                     LCLMSDLTELTALQKQIRWKENLAALGEMSAGIAHEFKNALATISGYAQMIRGESAPG
                     ETRDSAERILDQTRALTHVVTEFLRFAKPLEISYETVVMQPLVERVAEELRESRPECD
                     VKCEGAYVELPGDEALLRQALVNLTRNAAEAARDGAARGRITISGTVEELGGRKWQRI
                     CVADNGPGIPENELPKIFLPFFTTKSEGTGLGLAVVQKVALQHGGSIEARNCPDGGAE
                     FLLWLPLRQESLPSLFPSPANRI"
     gene            1761..2393
                     /locus_tag="VN788_09515"
     CDS             1761..2393
                     /locus_tag="VN788_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20806.1"
                     /translation="MRTLLQLTAVAIILGFAGSSWGQQATGNSAQSQSPTATSTQSST
                     TPSSTAQDPLAEAARRARERRKEERKTPRVFTNDNIPTSGGISSVGQPATPRESASPK
                     GTETRAPAATASSGNGEKFWRDKFASLRHKLQQDQENLALSQRELGTLNTQFYADPNK
                     QLQQQLTRDDIDKKTAEIDKAKAQVDADQKAISDAEDDLRKSGGDMGWAR"
     gene            2406..3794
                     /locus_tag="VN788_09520"
     CDS             2406..3794
                     /locus_tag="VN788_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU20807.1"
                     /translation="MEPVLLVEDKTELRAMLRKALERWGYAIDEAPDGNSAIDKVRSR
                     RYQMVLSDLKLPGNSGIDVLREARRAEPTLPVILVTAYGSVEEAVTAMKEGAFDFIQK
                     PVDLDHLRLLLDRAAKQQELLRENLLLREEYSARYGFPRIVAEHPAMKDASQMTQRVA
                     ATDSTVLLLGESGTGKELFARAIHHLSPRVEQPFVALNCAAIPEGLVENELFGHERGA
                     YTGAGARKMGKLELAHRGTLFLDEIGELPLAIQSKLLRVLEERKFERVGGTQEIEANV
                     RIVTATNKDLQRAVADKTFREDLYFRISAVPITIPPLRERGDDVVLLAEHFLERFRRE
                     FRKPTLELTPDARARLRSYAWPGNVRELQNAIERAAILANGSGIDTAALQLPSPKPAP
                     EELPDGMLEEQFLWDGPLDEVAQRAVAHVERFKIQNALRESKWNKTRAAEMLGVSYKT
                     LLTKIRALGLVN"
     gene            complement(3901..5064)
                     /gene="hemW"
                     /locus_tag="VN788_09525"
     CDS             complement(3901..5064)
                     /gene="hemW"
                     /locus_tag="VN788_09525"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HXU20808.1"
                     /translation="MSLGVYIQVPFCQTKCTYCNFHTGVVSRDRYEPYAAAVCREIAG
                     TSSTPPDTVDTVYVGGGTPSLLDPSALAKMLETLRGSYCAKSQPVPLEITLEADPETI
                     TSEKARAWIAAGFNRVSLGSQSFDNRELQAAGRMHRRADIFNAVESLRAADFDNISMD
                     LIAGLPHQTRDSWEESVSELLRIRPEHASIYMLEIDKDSRLGRESLAGGSRYGAGAIP
                     DDDTIADFYESARLRLDEAGYTHYEISNWGLPGRESRHNVKYWRREPYYGFGAGAHSF
                     DGMTRWANAHESSRYMACIEQGSSPREQFETLTPPQALDEEFFVGLRLLEGIDFARIE
                     REWEPELPKRLAAVRENIERLHSLGFLEVEGSRVRLVPDRLTVSNEVFMQLLG"
     gene            complement(5088..5360)
                     /locus_tag="VN788_09530"
     CDS             complement(5088..5360)
                     /locus_tag="VN788_09530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20809.1"
                     /translation="MNSRDWPIRTATVLYAECANVAALGCEVATLHYNYTVDGEKYSG
                     AYEKPFVFHSSGVDYANQFAKGMQFKLRVKPGKPKVSVPFDERRPF"
     gene            5525..6094
                     /locus_tag="VN788_09535"
     CDS             5525..6094
                     /locus_tag="VN788_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HXU20810.1"
                     /translation="MGKGRLEAFTDGVIAIVITIMVLELTVPSGSGFSSLAARLPVFL
                     VYALSFANVGIFWNNHHHMLHVTDRVNGTVLWANLFMLFWISLVPFAIRWMDEAHFAA
                     TPTALYGVVLAMAAFAYILLERAIIACNGPDSKLARAVGRDRKSVLSLSLYVLAIALA
                     FVHPWIAIALYVVNALIWFVPDRRIESAV"
     gene            complement(6364..7284)
                     /locus_tag="VN788_09540"
     CDS             complement(6364..7284)
                     /locus_tag="VN788_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20811.1"
                     /translation="MRTLLKCASLLLLSLYFLTPVPSSAQVAVSVRIGPPALPVYAQP
                     PCPEDGYIWTPGYWAYGDAGYYWVPGVWVAPPQAGLLWTPGYWGFETGFYRWHPGYWG
                     PHVGFYGGINYGFGYTGVGFVGGRWIGGHFAYNTAVVQVNRSVVRNVYVDRTVIRHED
                     NRVSFNGRGGVMARPTAREESFEHERHFEATNNQFSHERAAREDRNQSASFNHGRPNT
                     VGMGSVNERRDNQQQRIANGVRSGQMTAGEAARAERHEQNINRHVEADRSANGGRLTQ
                     QERHNINERQNNASRQIRNEKHNDRTAHGR"
     gene            complement(7456..7842)
                     /locus_tag="VN788_09545"
     CDS             complement(7456..7842)
                     /locus_tag="VN788_09545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU20812.1"
                     /translation="MPFRIMIADDEPHIRTALRTIVERHEDWTVCGEAVDGIKAIEKA
                     EELHPDLILLDVSMPNLDGISATPQIRKRVPETGILILTLHESIDLARMAAKAGAWGF
                     VTKSLASTDLVPTIEAFQASVSVAPR"
     gene            complement(8049..>8158)
                     /locus_tag="VN788_09550"
     CDS             complement(8049..>8158)
                     /locus_tag="VN788_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III polyketide synthase"
                     /protein_id="HXU20813.1"
                     /translation="VLEDVYKHRRPDPGTLSILAAMGPGFCSELVLLRW"
BASE COUNT         1718 a         2462 c         2350 g         1628 t
ORIGIN      
        1 tgcgcgggcg cgagggaatg ggcatgggag acgtgaagat gatgctcatg gcaggcgcgt
       61 ttttgggcgc gaagcggacc ttgctgacta tttttgcggg atcgcttctg ggcagcattc
      121 tggggctcgc attcatggcc gcgcggagca aggactccaa ctacgaattg ccctttggga
      181 ccttcttggg aatggcggcg ctgctggtag tgttcttcgg gacgccggtg gtgagttggt
      241 atcagtcgat actcttagga cgatgacgag cgagagtggt aacgagcgat gaacgcaata
      301 gttcccataa atcacaccgc cgtgccgacg gcaggcttgg tgtatgcggc cgcgacgctc
      361 gcaatcctgg tggtggcgct cttggtgctt tttattatcg cgcgaaaatt cctgcgcaag
      421 ccgggtgccg cgcccgaagc ggattgggcg aagcccaagg cagacatgga gaatgcatcg
      481 gctttcatgg cggcctcgat gcagggtgtg atcgaaaagc ttcgcgcgca ggaaaaggaa
      541 cttgcgcggc tacatatgct ggcgcaagaa cgcgcgcagg agtcggagcg gctaaccgaa
      601 gaagtgacgc ggcacatgcc gaccggaatg cttttggtaa acgcaacggg cgccattagt
      661 tcgacgaatc cggcggccga agacgcactg gggcgccgac cggtccgcta tcggtcctac
      721 aaggagattc tcggcgacga ttcgggactt tcacagatgc tcaccgcttg tctgcgcgat
      781 ggaaaaacct ttcagcgcgg cgaggtggaa catcttacca atgacggcga agtccggcgg
      841 ctgggcgtca ccatttctcc gatctaccgt cccacgcgcg cggctgtgcg gtcccgcgtt
      901 ccaggtgcga cggagcagcc ggagatgaag gtgagcggcg ctctgtgcct gatgagcgat
      961 ttgaccgagc tcaccgccct gcaaaagcag atccgctgga aagaaaatct tgcggccctt
     1021 ggcgaaatgt ccgcgggcat tgcgcacgaa tttaaaaacg cgctggcgac catttccggt
     1081 tacgcgcaga tgattcgcgg cgagtccgcg ccaggtgaaa cgcgcgattc ggccgaacgc
     1141 attctggacc agactcgcgc gctcacgcac gtggtcacgg agtttctgcg tttcgcgaag
     1201 cccctggaaa tcagctacga gacggtggtg atgcagccgc tggtggagcg cgtggccgaa
     1261 gaattgcgcg agtcccggcc tgagtgtgac gtgaagtgcg agggcgcgta cgtggaactg
     1321 cccggcgacg aggcattgct ccggcaggcg ctcgtgaatc tcacgcgtaa cgccgcggag
     1381 gcggctcggg acggcgccgc gcgaggccga atcaccatct cgggcaccgt cgaggaactc
     1441 ggcggccgca aatggcagcg aatctgcgtg gcggataacg gccccggcat tcccgaaaac
     1501 gagctgccga aaatcttttt gccatttttc accaccaaat ccgaaggcac cggcctggga
     1561 ctcgcggtgg tacaaaaagt cgcgctacag cacggtggca gcatcgaagc acgcaattgt
     1621 cccgatggcg gagcagaatt cctgctgtgg ttacctttgc ggcaggagtc tttgccgtcg
     1681 ctcttcccct ccccggcgaa ccgcatctaa actgattgag ccatattgaa ccgggcggag
     1741 gcaccgcaag gagcgtagcc atgcgcacat tattacaatt gaccgcagtg gcgatcattc
     1801 tgggattcgc gggcagcagc tgggggcagc aggcgaccgg taattccgcc caatcccaat
     1861 ctcccacggc aacttcgacg cagtcctcca cgacgccatc atcgaccgcc caggacccgc
     1921 tcgccgaggc cgcgcgtaga gcgcgcgagc gacgcaagga agagcggaaa accccgcgag
     1981 tatttacaaa cgacaacatt cccacctcgg gcggcatctc gtccgtgggt cagcccgcga
     2041 cgccccgcga atcggcatct cccaagggca cggaaacccg cgcgcctgcc gcgacagctt
     2101 cgtcaggaaa cggcgaaaag ttctggcgcg acaagttcgc aagtttgcgg cacaagctcc
     2161 aacaggatca ggaaaatctc gctttgtcgc agcgcgaact aggcacgctg aacactcagt
     2221 tctacgccga tcccaacaag cagttgcaac agcagttgac gcgcgacgat atagacaaaa
     2281 aaaccgctga aatcgacaaa gctaaggccc aggttgatgc cgaccagaag gccatttccg
     2341 acgccgaaga cgatctgcgc aaatccggcg gcgacatggg ctgggcgcgc tagccccttc
     2401 ggcgaatgga acccgttctc cttgtcgaag acaagaccga gctgcgagcc atgctccgca
     2461 aggcgctcga gcgatggggc tatgcgattg acgaagcgcc cgacggcaat tccgcgatcg
     2521 acaaagtccg cagtcgccgc tatcagatgg ttctctccga tttaaaactc cccggaaatt
     2581 ctggaatcga cgtgctgcgc gaggcccgcc gcgcggagcc aaccttgccc gtgattctgg
     2641 tcaccgcgta tggctctgtc gaagaagctg tgaccgccat gaaagagggc gcgttcgatt
     2701 ttatccagaa gccggtggat ctggatcacc tgcgcctgct gctcgatcgc gccgcaaaac
     2761 agcaggagct tctgcgcgaa aacttgctgc tacgcgaaga atattcggcg cgctacggat
     2821 ttccgaggat cgtggccgag catccggcca tgaaagacgc cagccagatg acccagcggg
     2881 tcgcagccac cgattccacc gttctgctgc tcggcgaaag cggcaccggc aaggaactct
     2941 tcgcccgcgc catccaccat ctgagcccgc gtgtcgaaca gcctttcgtc gcgttgaact
     3001 gcgcagcgat tccagagggg ctggtcgaaa atgaactttt cgggcacgag cgcggcgcct
     3061 ataccggcgc aggagcgcga aaaatgggga agctcgagct cgcgcatcgc ggcacgcttt
     3121 tcctcgatga aattggcgag ctgccactag cgattcaaag taagctgctg cgcgttctcg
     3181 aggaacgcaa attcgagcgt gtcggtggca cccaggaaat cgaagccaac gtgcgcatcg
     3241 tgaccgccac gaacaaagac cttcaacgcg ccgtggccga caaaaccttc cgcgaagatc
     3301 tttatttcag aatttcggcc gttcccatca ccattccccc gctccgcgag cgcggagacg
     3361 acgtggttct gttggccgag catttcctcg aacgctttcg gcgcgaattt cgcaagccga
     3421 ccctcgaatt gacacccgac gcccgtgctc gcctgcgcag ctacgcgtgg ccgggcaacg
     3481 tgcgcgaatt gcaaaatgca atcgagcgcg cagccatcct ggcaaacggc tccggaatcg
     3541 ataccgccgc gctccagttg ccttccccca agccagcccc cgaggaattg cccgatggca
     3601 tgctcgaaga acagtttttg tgggatggac cgcttgacga agtggcgcag cgggcggtcg
     3661 cgcacgtcga gcgattcaag attcaaaatg ctctgcgcga atccaagtgg aacaaaacgc
     3721 gcgctgccga aatgctcggc gtctcctaca aaaccctgct aacgaaaatc cgcgccctcg
     3781 gcctcgtcaa ttagcttcgg cctttgccgg tgcgcatctg tccgtcgcgg tacggcgtca
     3841 ttctgagcgg agtgtgccga agaatctctc ttcgacgccg gccgaaactg cgcgccacaa
     3901 ttaccccagc agttgcataa acacttcatt cgaaaccgtc agccgatcag ggactagcct
     3961 cacgcgcgac ccttctactt ccaaaaatcc aagcgagtgc aatcgctcaa tgttttcgcg
     4021 caccgccgcc agcctcttcg gaagctccgg ctcccactcg cgctcgatcc tcgcaaaatc
     4081 aatcccttcg agcaaccgca agcccacaaa aaactcttcg tccaatgcct gtggcggcgt
     4141 cagcgtttca aactgctctc gcggcgaact tccctgctca atgcacgcca tgtagcgcga
     4201 cgactcatgt gcattcgccc agcgcgtcat cccatcgaac gagtgtgcgc ccgcgccaaa
     4261 tccgtaatag ggctcacggc gccaatattt tacattgtgg cgcgattcgc gccccggcaa
     4321 tccccagttt gaaatttcat aatgcgtata gcccgcttcg tccagacgca gccgagcaga
     4381 ctcgtaaaaa tcggcaattg tatcgtcatc gggaattgcg cccgcgccgt agcggctgcc
     4441 tcccgccagc gattccctgc ccagccggct gtccttatca atttccagca tgtatatcga
     4501 cgcgtgctcg ggacgaatcc gcaaaagctc gctcaccgat tcttcccacg agtcgcgcgt
     4561 ctggtgcggc aacccggcaa tcaagtccat gctgatattg tcgaagtcgg ccgcgcgcaa
     4621 ggattcgacc gcgttgaaaa tatcggcgcg acggtgcatc cgtcctgcgg cctggagctc
     4681 gcgattgtcg aatgactgcg acccaaggct gacgcgattg aaccccgccg cgatccacgc
     4741 gcgagccttt tcagaagtta tcgtttcagg atcggcttcc agagtaattt ccaggggcac
     4801 gggctgcgat ttcgcgcaat agctcccgcg aagcgtctcc agcatcttcg ccagcgcgct
     4861 tggatcaagc aggctgggcg tcccgccccc aacgtagact gtgtcaacgg tgtccggcgg
     4921 agtcgaggat gttccggcga tctcgcggca tactgccgct gcatatggct catagcggtc
     4981 gcgcgacacc acacctgtgt ggaaattgca gtaggtgcac ttcgtctggc agaaaggaac
     5041 ctgaatgtaa acgccgaggc tcatcagaag ttacgagttt agcgcattca gaatggccgc
     5101 cgctcatcaa acggaaccga cactttcggc tttccaggct tcactctcag tttgaactgc
     5161 atcccttttg cgaactgatt cgcgtaatcg acacccgaac tgtgaaaaac aaacggcttc
     5221 tcataggcgc cactatactt ctcaccgtcc actgtgtagt tgtaatggag ggttgcaacc
     5281 tcgcagccga gcgccgctac gtttgcgcac tccgcataca gcacggtcgc ggttctgata
     5341 ggccaatctc gactgttcat taatctgatc aagcgcagaa atatcctgaa tagccattcg
     5401 agaaaaacgt ctgcaaccaa accgcccatt cgtggcagaa gtgtaccaga gaccggaagt
     5461 catgtaatcg gcgtcgtgcc cacgcgacca aaggcgtatc tgataacatc ggcaaaggaa
     5521 ggaaatgggt aagggacgcc tcgaagcctt caccgatgga gtgatcgcca tcgtaatcac
     5581 catcatggtg ttggagctca cggtgccctc aggatctggc ttctcctcgc tcgcggcccg
     5641 cctccccgta tttttggtgt acgcgctgag cttcgcgaac gttgggatct tttggaataa
     5701 ccatcaccac atgttgcatg tcaccgaccg tgtcaatgga acggtgctat gggccaatct
     5761 cttcatgctt ttctggattt cgctggttcc ctttgcgatt cgttggatgg acgaggctca
     5821 ttttgccgcg acgccaacag cgctctacgg cgtagttctg gccatggccg cgtttgccta
     5881 cattctgctc gagcgagcca tcatcgcgtg caacggcccc gattcgaaac ttgctcgagc
     5941 cgtcggccga gatcgaaaaa gcgtcctatc cctttcgctt tatgtcctgg cgattgcgct
     6001 cgcctttgtt catccgtgga ttgcgatcgc gttgtatgtc gtcaacgcct tgatatggtt
     6061 cgtgcccgac cgccgaatcg aatcggccgt gtagcacaca gcgtggttta ctaattaacg
     6121 cgatcggaat tcccctcccg tgtcagtacg gaggaatccg aggccggcgc aatatgaaaa
     6181 ttgcgcgaag gctggctgaa actgtgcatt cacagcgtgc gtccgctggc gcaagcagcg
     6241 gagccgcacc gcctactcga aggacgggag accgtcgcag agttacttat acagcctagc
     6301 tgagcacgag tagatttcgt ggcgctaggc ccgaaggccc agcgccactt cgtcgtaagc
     6361 tccttagcgg ccgtgcgcgg tccgatcgtt gtgcttttcg ttgcggattt ggcggctggc
     6421 gttattttgg cgttcgttga tgttgtggcg ttcctgttga gttagcctgc ctccattggc
     6481 gctgcgatcg gcctcgacgt ggcggttgat attttgctcg tggcgttccg ccctggctgc
     6541 ttcgccggcc gtcatctgac cgctgcgaac gccattggcg atgcgctgtt gctggttgtc
     6601 gcggcgttcg ttgaccgagc ccataccgac cgtgtttgga cgcccgtgat tgaaggaggc
     6661 ggactgattg cgatcctccc tggctgcgcg ttcgtgcgaa aactgattgt tggtggcttc
     6721 aaagtgccgc tcgtgctcga atgactcttc gcgcgcggtg ggcctggcca tcacgccgcc
     6781 tcgcccgttg aagctgactc gattgtcttc gtggcggatg acggtcctat cgacgtacac
     6841 attcctgacc acgctccgat tcacttggac gacggcggtg ttataggcga agtgtccgcc
     6901 gatccagcgt ccaccgacga agcctacgcc ggtgtagccg aagccgtagt taatgccgcc
     6961 gtagaagccg acgtgaggtc cccaataccc tgggtgccac cgataaaatc cggtctcgaa
     7021 gccccaatac ccgggtgtcc atagcaggcc agcctggggc ggagcaaccc acactcccgg
     7081 aacccaatag tagccggcgt cgccataagc ccagtagccg ggcgtccaga tatatccgtc
     7141 ctcggggcac ggtggttgcg cgtacacggg caaggctgga ggaccgattc gcaccgagac
     7201 cgcgacctgc gccgaagacg gcaccggcgt taggaagtaa agtgacagca gcaaaagact
     7261 tgcgcatttc agcaaggttc tcatcgccac ctccagtagg ttcttcttca ctatagtcaa
     7321 acacgcgcca gcgcaaaaag tttccccctg gccgcttccc gagcgacatt gcaaggaaca
     7381 gcacatcgcc aaaatttgga cgcctcggat ggagggcaaa ttgtgcgggg cgaggacctc
     7441 ttcgaggcta aggccctatc ttggagctac cgaaaccgac gcctgaaatg cctcaatggt
     7501 cggtactaag tcagttgacg cgagcgattt cgtgacgaaa ccccaggcgc ccgccttggc
     7561 ggccatgcgc gcgagatcga tggactcgtg aagggtaaga atcagaatac ccgtctcggg
     7621 gactcttttg cgaatttgag gtgtagcgct gatgccatcc agattaggca tcgagacgtc
     7681 cagcaagatc aggtccgggt gaagttcctc ggctttttca atggccttga tgccatcaac
     7741 agcttccccg caaacggtcc aatcttcatg gcgctcgacg atggttcgca gcgccgttcg
     7801 aatgtgtggt tcgtcatcag ctatcataat tcgaaaaggc acggttcgat tgctcctgga
     7861 gttctaggac agtctgacgc gaacgaagct tgcaaaaaca tccagcgttt gccttagtac
     7921 gtaactcgat aggcacactc ccggtgaaca accggcgaaa caaacggctt gcacgcgaac
     7981 cgcaggcgaa cccttcgcgg tctttgcctc ggcgtgaaaa acgatctcgc gaaattatcg
     8041 gcttttcgct accagcgcag caggaccagt tccgagcaga accccgggcc catcgcggcc
     8101 aggatgctca acgtgcctgg atccgggcga cggtgcttgt acacatcttc cagaacca
//
LOCUS       DATNDT010000298        20828 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3480130, whole genome shotgun sequence.
ACCESSION   DATNDT010000298 DATNDT010000000
VERSION     DATNDT010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 20828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20828
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3480130"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..730)
                     /locus_tag="VN788_09555"
     CDS             complement(<1..730)
                     /locus_tag="VN788_09555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXU20814.1"
                     /translation="MADSNGTAFRSESKLDGKGSHGYLAILLFASAVYLGCIVSPPSL
                     MDDVDAVQAQIARNMLASGDWVTAHIDGLAYLEKPPLLHWITAISYKVFGVHDWSARV
                     PIALSAIALCLLTAAFGTWAFGKRAGIYSGLCMSTCAGLFLFTRILIPDVTLTLTVAL
                     ALWAFLRALNPDELHPTFWAFLMAASLGIGLLLKSLIAVVFPVAAGGLYLLVTRQLFS
                     ARTWKRLHPFTGLLVLLLIAAPWHI"
     gene            complement(658..2586)
                     /gene="asnB"
                     /locus_tag="VN788_09560"
     CDS             complement(658..2586)
                     /gene="asnB"
                     /locus_tag="VN788_09560"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXU20815.1"
                     /translation="MCGIAGFTHKDWSPESDRIDRVAATILHRGPDQQGIFRSNVASL
                     AATRLKIIDLAAGDQPIKSDDGNAVIVFNGEIYNHFELRGELEQLGHRFRSRADTETV
                     LHAFLEWDTNCFRKLRGMFAIALWDETKQRLVLARDRMGIKPLYVARQGKDLFFGSEL
                     KAILIHPEIDRRLSMSGLSCYLSLNYVPCPWTLVEGIEKLPPGFWLEWCGGEIHSEVY
                     WDLPFVPPRQLTMNAAKEELDSLLAQAVREHLLSDVPLGVWLSGGIDSSTILHYAAKV
                     SGSKLKTLSISFRGRSFDETPYIREVAGWYGTDHEQFDLNPGENLAGTIEEFAYYSDE
                     PTADAGALPVWFLSKMSKKKVTVTLSGEGADEVFGGYLTYRANRLAGIARQFPSTAIR
                     AALCATSRWPVSDEKISVEYKLKRFLEGCLLQPDQAHVYWNGTFSDAQKRALLRSRLP
                     DSLDDTLQTLRERWIDTDNNLAATLWFDQKYFLPDDILNKVDRMSMAHGLEVRPPFLD
                     HRIVEFAASLPACFKVQGATQKVILKELMKDKLPASVLAKKKTGLDVPSHQWLRGPLR
                     LLMVNALNEGMADHPGLFEKSAIDATVRAHLDRRANLGYHLWGLMILFLWMRKWRIQT
                     EPLSAASQSSTERVLTAT"
     gene            complement(2623..3855)
                     /locus_tag="VN788_09565"
     CDS             complement(2623..3855)
                     /locus_tag="VN788_09565"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20816.1"
                     /translation="MPKAAISWPENWHLAVEALRANRLRAALTMLGVAIGSACIVLVI
                     TVSLTGKRYIAAQIEGVGSNLVYAGLEQSNHSQAITLSDQITLGDLEAVRESIPQVKR
                     VAGTNDVQMSVMANGKVWPVSLVGVTQQFQQIRKLLILRGRYFDDADFSNVSKVCLIS
                     QHLGQLTLPGGNPVGQSIHVGELTFTVIGVFTERIGTFGQSEIRTDTVLVPFPLVKYY
                     TGESFIETLYAQADRAADVPLVTSAAAQVLRERHRPEAEYDVENLSSILETSRRISFA
                     LTIVFLLVAMLALLISGIGIMNIMLVSVTERTREIGIRKAVGAQRREVLCQFLLEAIL
                     ISGIGAIAGIAIAVTIPFLVEALLRFLPVPGGLQIPISWISVALAFVVSCATGVLFGY
                     LPAKNAAQLQPVESLRYE"
     gene            complement(3967..5286)
                     /locus_tag="VN788_09570"
     CDS             complement(3967..5286)
                     /locus_tag="VN788_09570"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXU20817.1"
                     /translation="MPTRLDLQGTDLFFMLVLKVGWAAALAALLVRFRSFRNLVFTEN
                     RDSDQKVMLLLFLTPPLAIGVILRIFGYQFLDLTLEGSFLMGLIGGRIVGLLGGSLIS
                     LPAFGNHEWLSSPLAALVGLLAGMIRQSMPDKEDVWHFGPFQFLSIPSWLWKLMRYAK
                     GDWAMLPLFASAAFEAAQIVLAPRVPARWLFSLRPTNSGYAILVVLSSVMCVAMAVKI
                     WNNTRIEMNLEQNQQHLLKARMDALTSQINPHFLFNTLNTVSSLIRFDPDMARGVVLK
                     LSNILRRLLRQHDTFVALRDELEFIDNYLDIEVIRFGRDNLQIFKEIDEDAMDVFVPS
                     MLLQPIIENAIKHGLAPRLEGGKIYLRAHRRDGRLGIEVDDNGVGMSRERLLEVYGGG
                     IGISNVHERLRLLYGDQFEMDIRSEEGEGTHIRIAIPEVATVESAIF"
     gene            5436..6050
                     /gene="ribA"
                     /locus_tag="VN788_09575"
     CDS             5436..6050
                     /gene="ribA"
                     /locus_tag="VN788_09575"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HXU20818.1"
                     /translation="MKSKKRTSNAKVVATAGLPTRFGRFEVIGIKGRGVQDEAVAIRH
                     GRLKRGKAPVVRIHSQCLTGDVFASQRCDCRAQLELSLRKIAKAPSGIVLYLPQEGRG
                     IGLINKLKAYELQDEGLDTVEANRRLGFAADSRNYEFAAAALKALDVHSVRLLSNNPD
                     KIAQLERSGIRVVERIPCRPRANHHSRSYLRTKQSKLGHLLANL"
     gene            complement(6095..6295)
                     /gene="thiS"
                     /locus_tag="VN788_09580"
     CDS             complement(6095..6295)
                     /gene="thiS"
                     /locus_tag="VN788_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012522401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HXU20819.1"
                     /translation="MTITINGERREIPDDLNVAALLDHIGMARDRVAIERNLEILPRG
                     QWQQTAVQPNDNFEIVHFVGGG"
     gene            complement(6299..6916)
                     /locus_tag="VN788_09585"
     CDS             complement(6299..6916)
                     /locus_tag="VN788_09585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20820.1"
                     /translation="MSSGFNTDVRVGEQVYHVQTEDRGPNRPAIDTAVYQQGRVLHRR
                     SSNYAEFAASEEFTAEALRQRVEQQHREIIEELRSGTLTAGSAAPVEQPPASGIQIQL
                     LNAQAWLSAGNVSLEVEIRGRADQQPQAGVHVEARIDGALLNARHEGTTDDHGRARIQ
                     FPVPPLGKGDLALVIQASGHAGGDELRFSMRSRHKAAPVSSPPSS"
     gene            7029..7403
                     /locus_tag="VN788_09590"
     CDS             7029..7403
                     /locus_tag="VN788_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HXU20821.1"
                     /translation="MNGNYLNNYGPLLLMFILAAGLAGALITASTVIGRHKRTREKDQ
                     PYECGIRPTGDARQPFSVHFYMVGLIFILFDIEAIFLYPWALVYRQLSVFGFVEMVLY
                     IIILLVGYIFLWKKGALNWDQS"
     gene            7483..7905
                     /locus_tag="VN788_09595"
     CDS             7483..7905
                     /locus_tag="VN788_09595"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HXU20822.1"
                     /translation="MPRAIAEVIESKGETTIVVPAGDLLQISEFLATDPALLFSFLSD
                     ISAVDRFPLEPRFEINYHLLSLEKRARLRVKVRLPGRDPVVHSVTPIWPTANWHERET
                     FDLFGIRFDGHPDLRRILMPDEWEGYPLRKDYPVTGFR"
     gene            7927..9129
                     /gene="nuoD"
                     /locus_tag="VN788_09600"
     CDS             7927..9129
                     /gene="nuoD"
                     /locus_tag="VN788_09600"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HXU20823.1"
                     /translation="MNTTTLEMPVGPAQPMVLNMGPQHPSTHGVLRVILELDGEIVLK
                     ADPVIGYLHTGIEKSCEDKTYSQCITLTDRIDYLAPLSNNLGFCLAAEKLIGIEVPKR
                     AQYIRVLLTELTRITSHLVWLGTHAIDLGAMSVFLYSFREREEIMKIYEYVSGQRMMT
                     SYFRIGGLALEPPPGWLDRVQRVVDSLPGHVDEYEDLLTQNRIWIGRTKGVGFLSAQD
                     AIAMGVSGPLLRASGVAYDIRKAFPYSSYDEFDFNVQTQTAGDSYARYQVRVAEIRES
                     IKIVRQAMEKIPSEGPFRAEAPGIIPPSREEMKTSIEGLIYHFKIFTEGFAPPPGEVY
                     QVIESPRGELGVFMVSDGSAKPYRVKFRAPSFVNLQALPKLCEGRLLADVVACIGTTD
                     IVLGEVDR"
     gene            9151..10968
                     /gene="nuoF"
                     /locus_tag="VN788_09605"
     CDS             9151..10968
                     /gene="nuoF"
                     /locus_tag="VN788_09605"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HXU20824.1"
                     /translation="MMELPPQLDAKFNELISRYPVKRSALIPMMLYAQDHFGYLGDAI
                     LDEIATRLDLNKIQVEETLAYYSMLRRKPAGRYHIQVCTNISCMLRGGNELYHHVQKR
                     LGIGNKEVSASGTFSLEEVECMGACTGAPAMQVNYDFHEDLDPDKVDTIFEQLQDGRV
                     PQASPVTAGSLHNRLSAEVPVISKRFGIPNSRKIDVYLKNEGYQALEKALKQMTPEQI
                     IDEVKKSNLRGRGGAGFPTGMKWGFVPKSNDKPKYILCNADESEPGTCKDRPLMEMDP
                     HQLIEGMVIAGRAVSANRGFIYIRGEYRYIIDIMDRALAEAYAQGYLGKNIRDSGFDF
                     ELCTHTGAGAYECGEESALMESLEGKRGYPRIRPPFPAVVGLYGCPTVINNAETLSTI
                     APIIRQGGDWYASLGTPKNGGTRLYSISGHVNRPGIYELPLGFPLRRLIEEVAGGMRD
                     GKKLKAVIPGGSSCPLLSADEIDVAMDYDSVAKIGSMLGSGGTVIMDEDTCMVDMARR
                     IMHFYAHESCGWCIPCREGTAWLRKMLDRFHEGAGRAEDIPLIDELSKNMLGRTFCPL
                     GDAAALPTISIVKKWRNEFEDHLHGKCAYKSTDVLAAAH"
     gene            11141..13420
                     /gene="nuoG"
                     /locus_tag="VN788_09610"
     CDS             11141..13420
                     /gene="nuoG"
                     /locus_tag="VN788_09610"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HXU20825.1"
                     /translation="MAEQKQIAFSIDGRPVTTTAGTLVIEAAKTQGIEVPSFCYYPGY
                     SLQAACRMCLVEVEKMPKLQTACTLVATDGMVVKTTTDQVHKARKDMIEFLLSNHPLD
                     CPVCDKGGECELQDMTFRYGLDKTRFVEEKLHYPEEKWSPLVYYDAPRCILCFRCVRA
                     CDEGMDVKALGVGSRGVQSVILPNKPGELACEECGMCIDICPVGALTSGTYRYQTRPW
                     EMTYFPTPCTHCSNGCKTTLSVRNNEILRANNRDHSGFNGEFLCAKGRFGFDFTNHPE
                     RIRQPLIRRNGKLEPASWEDALEEVARRLKKVHKTHGPSSIGVIGSNHTTNEENYLLN
                     RFTRVTLGTNNLDHHRTADYAGLAAALGPRAKDSLATMADIYNAPAVLLVGNDVTQQN
                     PLVAWQIRAGFRHHGLRLYVLNGGPSKIHRQAKIAIELPAGTERQAVEWLASEKGQLE
                     PQVTESLVRLKAALEAEKDVVVLFGADIQGSAIRDLISLAARFGGATKFMALGDYSNS
                     RGAADMGILPDRLPGYALLSDAGERARFAKAWGGEISDAPGLTAAAMIEAALAGKLKA
                     LYVVGANPLKTFAATQPDKISGLDLLVVQDMFLTETAQRADVVLPAASSYEKDGTLTN
                     TAGEVQMTHRSIDPQGPRSDFDLIRILSHQLGTLGLGAPIRLRTPEAAFDEIGQHVSG
                     YNLSYPTLLAGGSEMSTPACKVAEPSYDAPPGAVFSSHDYLFTSGTLGRYCSRLTSTN
                     EAKERPWSSSPSTQSWWHR"
     gene            13378..14604
                     /gene="nuoH"
                     /locus_tag="VN788_09615"
     CDS             13378..14604
                     /gene="nuoH"
                     /locus_tag="VN788_09615"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HXU20826.1"
                     /translation="MEFISQHPVLVASLVKVAVLLFIVMTALAYLTWFERKVVARIQS
                     RWGPYYVGAHGLLQPLADGLKFLFKEDVTPPASDRFVYLLAPFLALSLALTALALIPF
                     GPPSVRFPNGQSVFVVSDIDIGLLFLFAITSIGVYGVALAGWSSNSKYPLMGSLRSSA
                     QMISYEVSLTMSVVGVLLLAGTLNFNEIISRQSGHFFLHWNVIHTGYGYFPQIIGFLC
                     YLTAAIAETNRIPFDLPEAETELVAGFHTEYSSFKFAMFFMAEYANMLTVSCLATILF
                     FGGWLSPLPDSWHWQLYLPGAGLLLLGLYTAYDTVANMRGIAQLQFAVVTALALGAGA
                     ICLIPVVMPIVQGPFWFTTKVLVFIFFYVWVRGTLPRFRYDQLMSFGWKLLLPVSLAN
                     LVITAFVIILPKLGHP"
     gene            14601..15110
                     /locus_tag="VN788_09620"
     CDS             14601..15110
                     /locus_tag="VN788_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HXU20827.1"
                     /translation="MSVPFFVFFACAVLAIAGALVLIISREPIHSALALILVMISLAV
                     LYLLLGAPFIAAVQIIVYAGAIMVLFVFVIMLLNAGVEERTNWSKIAKYAGLPLAVVL
                     LLEIAHWLFRAPIGAAIANGAGAPGPAASTAQLSLELFQQYLFPFEATSILILIAILG
                     ALVLARKEE"
     gene            15107..15415
                     /gene="nuoK"
                     /locus_tag="VN788_09625"
     CDS             15107..15415
                     /gene="nuoK"
                     /locus_tag="VN788_09625"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HXU20828.1"
                     /translation="MSVVPISWYLVLSAILFGLGVLAFVFKRNIITIFMSIELMLNAV
                     NLAFVAFARARNQLDGLVFVFFVIVVAAAEAAVGLAIVIMLARNRESLNVERINLLKF
                     "
     gene            15415..17421
                     /gene="nuoL"
                     /locus_tag="VN788_09630"
     CDS             15415..17421
                     /gene="nuoL"
                     /locus_tag="VN788_09630"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HXU20829.1"
                     /translation="MPILSHLWIILALPLAGSAINGLFGKRWPQSAVNSVAVGSVSLA
                     FLAAVELAREFSHLAQGQIPYIGNYFTWITAGSFRADFSLQVDQLTIVMVLTVSFVGL
                     LIHIYSTGYMAHEGGYYRFFSFLNLFMFFMLTLVMAANLIVMFVGWEGVGLSSYLLIG
                     FWFLKKSAADAGKKAFITTRIGDLGFMVGLLLVFWTFNSENFGDIFQQAGHMASESAT
                     HVGVLTAICLLLFAGAVGKSAQLPLYVWLPDAMEGPTPVSALIHAATMVTAGVYMVAR
                     MHPLFSRAPIAMFVVAVVGAVTAFYSATIGLMQTDIKKVLAYSTVSQLGYMFLACGVG
                     AYAAGIFHLMTHAFFKGLLFLAAGSVIHAMGGEQDMTKMGGLRKKIPVTYWTMLIATL
                     AIAGIPGFAGFFSKDEILEAAGSGPHANLWLWLLGLIGAGLTSFYMFRLVFLTFFGKP
                     RYDEHKVHVHESPKNMTVPLMILAFCSIFGGWFAAPHLVGGTEYFEHFLHPVFSSNAV
                     QAATEQAGSLAGNPGAQGGVASSPAMELLHALTGWPVIVAILGLLVAWWFYIKNPDAP
                     KRLAQKLHGLYILILNKYYVDEIYAALIIRPLLWISTNVLWHGVDQGVIDGIVNGSAR
                     VARESGGKVRELQSGNTRSYAAWVVIGAVGFTVLLLGILGMVAH"
     gene            17423..19147
                     /locus_tag="VN788_09635"
     CDS             17423..19147
                     /locus_tag="VN788_09635"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011941006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HXU20830.1"
                     /translation="MGTQPLLLTVITFLPLLSVAALLLLRSDDHLWIRRIALAASVAE
                     FIISLLLLRGFNSGTASYQFQEYHDWIGATIHYHLGIDGISLFLVLLTTFLTPLAILC
                     SWQSIRENVKGFFIALLVIETAMIGVFVSLDLFLFFVFWELTLIPMYFMIGMWGHGRK
                     IYAAVKFILYTMLGSILMLVAILWLYGKAGTFDLPQIQRLLQSGQIVFPPHTELLLFG
                     AFFLAFAIKVPLFPLHTWLPDAHTEAPTAGSVILAGVMLKLGTYGILRFCVPLFPDAA
                     HRMAPLIAVLAIIGIIYGALVATVQTDLKRLVAYTSVSHLGFVVLGIFAFNSISIQGA
                     IYQMLNHGVSTGALFLGVGMLYDRRHTFEIKQFGGLATPMPVLMSFFLFISLSSLALP
                     PLNGFIGEFLILIGTFAGHHAWAAWAASGTVLSAIYLLWAYQRVALGEVTVEKNRDLP
                     DASNRERLILATVAIVIIFMGVASPLFTRRMQASVNNVMQQISPPQNAQHRVAPPLRA
                     AETSSILGGRSFSSEKKPASYMRVNAWGQALLCFSAPCLISDSAVTFDRAAPRPLIAA
                     TAERRVQR"
     gene            19147..20637
                     /locus_tag="VN788_09640"
     CDS             19147..20637
                     /locus_tag="VN788_09640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HXU20831.1"
                     /translation="MFLSAEQHANLHRIAPEIYLCLFGLIIMVADPFVSAARKRVLGW
                     VGFVGTLVGLAAVYEASMHKGFAYSNLISNDDFSIFLHVIIIGVAALAILGSLDYLER
                     EGIQRGEYYALILFAAAGMGILAGANELVTAFVGLEMSSISTYVLIGFRRRALISNEA
                     SLKYFLLGSFATAFFLYGIAMVYGATGTTIIDKIQPALAAVSSGGRLPALAILGLGLT
                     FVGLGFKVVIAPFQIYGPDVYEGAPSPVTALLASAPKAAAFALMLRIFVVSFGAASDT
                     WFWAVWVSAILTMFIGNLAALVQTNVKRMLAYSSIAHAGYILVAFAAGTAIGVAAVLF
                     YLAAYVLMKVGAFLVVTHLGQRGEKRLTLQDYAGLGSKQPVLAACFSLFLLSLLGLPA
                     TGGFLGKFFAFQAALDASDAHGRWIIWLVVIAAINSVIGAYYYLRVMITMYFNEPSTD
                     YTPTRVAPALIVALVIAAAGTLYLGILPARVVDFAKVAADSLSLLH"
BASE COUNT         4167 a         6386 c         5531 g         4744 t
ORIGIN      
        1 ggatgtgcca aggcgcggca atcagcaaca ggaccaataa tccggtaaaa ggatgcaggc
       61 gtttccacgt ccgcgcggaa aatagctgcc gcgtcaccaa tagatacagc ccgccggcag
      121 caacagggaa tacaaccgcg attaggcttt tcagcagcag cccgatgccg aggctcgcag
      181 ccatcagaaa ggcccaaaag gtcgggtgca gttcgtcagg attgagcgcc cgcagaaaag
      241 cccataaggc cagcgccacc gttagggtca gcgtcacatc cggaatcaaa atcctcgtga
      301 atagaaatag tcccgcgcac gtagacatgc acaatccgga gtaaattccg gcgcgtttgc
      361 caaaggccca cgtaccgaac gcagctgtca gtaggcacag cgcaatcgct gacaaagcga
      421 tcggaacgcg cgcggaccaa tcgtgaacgc cgaatacttt gtaagagatc gccgtgatcc
      481 agtgcagaag gggaggcttc tcaagatagg ccagcccgtc gatgtgcgcc gttacccagt
      541 cgccggaggc gagcatgttg cgcgcgatct gcgcctgcac cgcgtcgaca tcgtccatca
      601 acgacggagg agaaacgatg cagccgagat acacagcgct ggcgaagagc aggattgcta
      661 ggtagccgtg agaacccttt ccgtcgagct ttgactcgct gcggaaagcg gttccgtttg
      721 aatccgccat ttcctcatcc acaagaacaa gatcatcaaa ccccaaaggt gatagccaag
      781 attggcacgc ctatcgaggt gagccctcac ggttgcatcg attgcgctct tctcgaaaag
      841 tccggggtga tccgccattc cctcgttcaa agcattgacc atcagaagcc gcagcgggcc
      901 gcgcagccat tgatgcgatg ggacgtccag cccggtcttt ttctttgcca gtacggaagc
      961 cggcagtttg tccttcatca actccttgag aatgactttt tgcgtcgccc cctgaacctt
     1021 aaaacacgcc ggcagcgacg cggcgaattc aacaatgcga tgatccagaa aaggcggccg
     1081 cacttcgaga ccgtgagcca tgctcatgcg gtccactttg ttcagaatgt cgtccggtag
     1141 gaaatacttt tgatcgaacc agagagttgc ggccaggttg ttgtcggtat cgatccagcg
     1201 ctcgcgcagt gtctgcagag tatcgtcaag cgaatccggc agtcgcgagc gaagcaacgc
     1261 tcttttttgc gcgtccgaga aggtaccgtt ccaataaaca tgggcttgat ccggctggag
     1321 cagacagcct tcgaggaacc gcttcagctt gtactccaca ctgatttttt catcggaaac
     1381 cggccagcgg ctcgtagcgc acagcgccgc tcgaatggcg gtagatggaa attgccgtgc
     1441 gattccggct aggcgatttg cacgatacgt caggtagccg ccgaagactt catcggcgcc
     1501 ttctccgctc agagtcacgg taactttttt cttgctcatt tttgagagga accaaacagg
     1561 cagcgccccc gcgtcggctg tcggctcgtc ggagtaatag gcgaattcct caatggtgcc
     1621 ggccaggttt tcgcccggat tcaaatcaaa ttgttcgtga tccgttccat accagccggc
     1681 aacttcgcgg atgtagggag tttcatcgaa tgaacgacct cgaaatgaaa tcgaaagggt
     1741 ctttaatttg gaaccggata cctttgcggc atagtgaagg attgtcgagg aatcgatgcc
     1801 tccactcagc cacacgccca gcggcacgtc cgataaaagg tgctcgcgca cggcttgcgc
     1861 cagcagggaa tccagctcct ctttcgcggc gttcatcgta agctgtcgcg gcggaacgaa
     1921 aggcaggtcc cagtaaacct ccgaatgaat ctcgccccca caccattcga gccagaagcc
     1981 gggcgggagc ttttctattc cctccactag tgtccagggg catggaacgt aattcagcga
     2041 taagtagcaa ctaagtcccg acatgctcag ccggcggtcg atctccggat gtatcaggat
     2101 ggcttttagc tcagagccga aaaacaaatc ctttccttgg cgcgcgacat aaagcggttt
     2161 gattcccatc cgatcgcggg ccagtaccag gcgctgcttg gtttcgtccc acagggcaat
     2221 cgcaaacatg ccgcgcaatt tgcggaagca attggtatcc cattccaaga aggcatggag
     2281 cactgtttcc gtgtctgcgc gggaccgaaa tcgatgcccc agttgttcca attccccgcg
     2341 cagttcaaag tgattgtaaa tctcgccgtt gaatacaatc acggcattgc catcatccga
     2401 tttgatcggc tggtcgccgg ccgcgagatc gataattttc aggcgtgtcg cggcgagcga
     2461 ggcgacgttt gaacggaaaa tgccttgctg atccggacca cgatgcagaa tcgtagcggc
     2521 caccctgtcg atgcgatcgg attcaggtga ccagtcttta tgcgtaaagc cggcaatccc
     2581 acacacttcc cgacccccta acgagagcga taggcccgtg tcctattcat agcgtagaga
     2641 ttcaacaggc tgaagctgag cggcgttttt agctggcaag taaccgaaga gcacgcccgt
     2701 ggcgcaggat actacaaacg ccagtgccac ggaaatccac gaaatcggaa tctgtagccc
     2761 tcctggaacg ggtagaaacc tgagcagggc ctcgaccaag aagggaattg tcaccgcgat
     2821 cgcaattccc gcgatcgcgc cgatgccgct gataagaatg gcctcaagca gaaattggca
     2881 gagtacctcg cgccgctggg cgcctaccgc tttgcgaatg ccgatttcac gcgttctctc
     2941 cgtcacactc accagcatga tattcatgat gccgatgccg ctgattagca gcgccagcat
     3001 ggcaaccaga agaaaaacga tcgtcaacgc gaacgagatt cggcgagacg tttccagaat
     3061 cgatgaaagg ttttcgacgt cgtattctgc ctcgggccgg tgccgctctc tcaacacctg
     3121 cgctgccgcg ctcgtgacta gcggcacatc tgcagcgcgg tccgcctggg cgtaaagcgt
     3181 ttcgatgaaa gactcccctg tgtagtactt caccaacgga aatggaacga gaacagtatc
     3241 tgtcctgatc tcagattgcc cgaatgttcc tattcgctcc gtaaaaaccc cgatcaccgt
     3301 aaaagtcagt tcaccaacgt ggatgctctg acctacagga ttgccgcctg gcagagtgag
     3361 ttgccccaaa tgttgggata tcagacaaac cttgcttacg ttcgaaaagt ccgcatcatc
     3421 aaaatagcgg ccccgcagaa tgagcagctt acgaatttgc tgaaactgct gcgtcacgcc
     3481 aaccaggctg acgggccaca ccttcccatt ggccatcacg ctcatctgca cgtcgttcgt
     3541 gcccgcgacc cgcttgacct gcgggatgct ttcgcgaacg gcctcaagat ccccgagggt
     3601 tatctggtcg gagagggtga tcgcttgaga gtggttcgat tgttccagac cggcatagac
     3661 gaggttcgac cccacgcctt cgatttgagc cgcaatgtat ctcttcccgg tcagcgatac
     3721 cgtgatgact agaacgatgc atgcgctccc aatcgcgacc cccagcatcg tgagcgcggc
     3781 gcgcaatcgg ttggcgcgca gcgcttcgac tgccagatgc caattttccg gccaacttat
     3841 tgccgccttg ggcaatattg ctcctccaat ctcccggccc ccagagaatc ggatgccatt
     3901 ttcggatccg acgacaccct gaagaagctc cgctctaaaa ttcctttgtt gaaaggagca
     3961 gtcgccctag aaaatcgccg attccacggt agctacttcg ggtatggcga ttcgaatgtg
     4021 agtgccttcg ccttcctcgc tgcggatgtc catctcgaat tgatccccgt agagcagccg
     4081 caggcgctcg tggacattgc ttatgccgat tccgccgccg taaacctcca aaaggcgctc
     4141 ccgcgacatt cccacgccgt tgtcgtctac ttcgatgccc agccgcccat cccgccggtg
     4201 cgcacgcaga tagatcttcc cgccttcgag gcggggcgcc agtccatgtt tgatcgcatt
     4261 ttcaatgatg ggctgaagca gcatgctggg gacgaaaacg tccatggcat cctcgtcaat
     4321 ttccttgaag atttgaagat tatcgcgccc gaagcggatc acctcgatgt caagatagtt
     4381 gtcgatgaac tcgagctcgt cgcgcaaggc cacaaaggta tcgtgctgcc tcagcagccg
     4441 gcgcaagatg ttcgaaagct tcaggaccac gccacgagcc atgtcggggt cgaagcggat
     4501 caacgaagac acggtattca gcgtattaaa aaggaagtgc gggttgattt gactggtgag
     4561 agcatccatc cgcgccttca gcaagtgctg ctgattttgc tccaagttca tctcgatccg
     4621 cgtgttgttc caaattttta ccgccatggc gacacacatc accgacgaca gcaccaccag
     4681 aatggcgtaa cccgagttag taggacgcag tgaaaacagc catctcgcag gaacgcgggg
     4741 cgccaatacg atctgagcgg cctcgaaggc agccgacgca aacaatggga gcatggccca
     4801 gtcgcccttc gcatatcgca tcagtttcca gagccacgat gggatgctca aaaattgaaa
     4861 tgggccgaaa tgccacacgt cttccttgtc tggcatcgat tgccggatca tgcccgccaa
     4921 caatcccacc agcgccgcca gcggactgga tagccactcg tgatttccaa acgcgggcag
     4981 gctgatgagc gatccgccga gcagaccgac gatccgtccg ccgatcagcc ccatgaggaa
     5041 ggatccttcg agcgtcaaat cgaggaactg atagccaaag atacgcagga tcaccccgat
     5101 cgccagaggg ggagtcaaaa aaagcagtag catcactttc tgatctgagt cgcggttctc
     5161 ggtaaaaacc aggtttcgga aagatcgaaa tcgaaccagc agtgcggcaa gcgccgccgc
     5221 ccatccgacc ttcaggacca gcatgaagaa caggtccgtg ccctgtaagt ctaagcgtgt
     5281 tggcataatc tgccggacgt cccggaaaat cctagtttac ggtagccaat gccggcgggg
     5341 atgtaaagag aagctgtcgc ccctgggtgg cgggttcgct ccatggtcgc gctacaccag
     5401 cgggcttttc gaccctgcta taatcattgg cttacatgaa gagcaaaaag agaacaagta
     5461 atgcgaaagt cgtcgctacc gctggtttgc ccacgcgctt cggccgcttc gaggtcatag
     5521 gcatcaaagg acgcggggtg caggatgaag cagtcgccat tcggcatggc cgattgaaac
     5581 gcggcaaagc gcccgtggtc cgaatccact cgcagtgcct tacgggcgac gttttcgcct
     5641 ctcagcgctg cgactgccgc gcccagctcg agctttctct tcgcaaaatc gcgaaagctc
     5701 catccggaat tgtcctctat cttccgcagg agggccgcgg gatcggcctg attaacaagt
     5761 tgaaggccta cgagcttcag gacgaagggc tcgataccgt cgaagcgaat cgtcgcctcg
     5821 gcttcgccgc ggattctcgc aactatgagt tcgccgccgc cgccctaaag gctcttgatg
     5881 ttcattccgt tcggttgctt tccaacaacc ccgacaaaat cgcgcaactc gagcgcagcg
     5941 gcatccgcgt ggtcgagaga attccgtgcc ggccccgtgc caatcatcat tcgcgctcct
     6001 atctccgcac caaacagagc aagctcggtc acttgttggc taacttgtag attgaaaaac
     6061 gacgaataaa tactgtctcg aaatcgagtt ccgcttagcc gccgcctacg aagtgcacga
     6121 tctcgaagtt gtcgttcggc tggaccgctg tttgctgcca ttgcccccgc ggaagaattt
     6181 caagattgcg ctcgattgcc acgcggtcac gcgccattcc gatatgatcg agcagtgccg
     6241 caacgttcaa atcatccgga atttcgcggc gctcgccgtt aatcgtgatg gtcacggtct
     6301 acgaactcgg cggggaactg accggcgcag ctttgtggcg agagcgcatc gagaagcgta
     6361 gctcgtctcc accggcgtgt ccgcttgcct ggatcaccaa cgccagatcg ccttttccaa
     6421 gtggtggcac cggaaactga attcgcgcgc gcccgtggtc atcagttgtg ccctcatgac
     6481 gagcgttgag cagggctcca tcaatccttg cctctacatg aacgccggcc tgcggctgtt
     6541 ggtctgctcg tcctcgaatt tccacttcca atgacacgtt ccccgccgag agccacgctt
     6601 gagcgtttag aagctggatc tggatgccgc tggcaggcgg ctgctcaacc ggcgcggcag
     6661 atcccgccgt cagcgtccct gaccggagct cttcaataat ctcgcggtgc tgctgctcta
     6721 cacgctgccg caacgcttcg gcagtaaatt cctcggacgc cgcaaattcc gcgtaattcg
     6781 acgatctccg gtggagcaca cgtccttgct gatacacagc tgtatcgatg gcgggacggt
     6841 ttggaccccg gtcttccgtc tgcacgtggt aaacctgctc gcctactcgc acgtcggtgt
     6901 taaacccgga actcacggcc tggcgatctc ctcctggaac ctgtttctca ggctgttcgg
     6961 acgcttaaaa ttctgggagt tcatttgacc ggcgtacagt cgaacgctat tatagcaaca
     7021 cggctaaaat gaacggaaac tacctcaata attacgggcc gctcttgctg atgtttatac
     7081 tggccgccgg actggccggt gcgttgatta ccgcttcgac ggtcatcggc cgccacaagc
     7141 gcacgcgcga aaaggaccag ccctacgaat gcggcattcg ccccacgggc gatgcgcgcc
     7201 agcctttttc cgtgcatttc tatatggtcg ggctgatctt tattttgttc gatatcgaag
     7261 cgatctttct ttatccatgg gcccttgttt atcgccagtt gagtgttttt ggctttgtgg
     7321 agatggtgct ctacattatc atcctcctgg ttggttatat ttttctctgg aagaagggcg
     7381 ctctcaactg ggatcagagc tagccgcccg tcgggaatcg gactaatagt ggaatctcag
     7441 gaactcgata aaaatccagc cgtgcggaag cttagggatt caatgccccg agccatcgcc
     7501 gaagtgattg agtccaaagg tgaaaccacc attgtcgtcc ccgcaggtga tcttctgcag
     7561 atttccgagt ttctggccac cgaccctgcc ctgctttttt cttttctttc ggatatcagt
     7621 gcagtggacc gctttcctct cgagcctcgt tttgaaatca attaccattt gctctccctt
     7681 gaaaagcggg cgcgccttcg cgtgaaagtc cggttgccgg gtcgggatcc cgtcgtgcat
     7741 tccgtcacgc ctatttggcc cacggccaac tggcatgagc gcgagacgtt cgatcttttt
     7801 ggtattcgct tcgacggcca tcccgatctt cgccgcattc tgatgcccga tgaatgggag
     7861 ggataccccc tccgcaaaga ttatcccgtt acagggttcc gttagaatgc agaaagggct
     7921 tgagttatga acactacaac tctcgagatg cccgtcggcc cggcccagcc gatggtcctc
     7981 aatatgggtc cgcagcatcc gtccacgcac ggagttctgc gcgtaattct cgaactcgat
     8041 ggcgaaatcg tcctcaaagc cgatcctgtc atcggctatt tgcacactgg catcgaaaaa
     8101 tcctgcgagg ataaaacgta ttcgcagtgc atcaccctga ccgaccgaat cgactatctc
     8161 gcgccgctct caaataacct cggcttctgc ctggccgccg aaaagttgat cggcatcgaa
     8221 gttcccaagc gcgcgcagta cattcgcgtc ctgctcacgg agttgacccg catcacctca
     8281 cacctggttt ggctcggtac ccacgccatc gatctcggcg cgatgtcggt tttcctttac
     8341 agcttccgcg aacgcgaaga gatcatgaag atttacgaat acgtttccgg ccagcggatg
     8401 atgaccagtt atttccgcat tggcggccta gctctcgagc ctcctcccgg ctggctggat
     8461 cgcgtgcagc gcgttgtcga ttcgctcccc ggtcacgttg acgaatatga agacctgctg
     8521 acgcagaacc gcatctggat cggccgaacc aaaggcgtcg gctttctcag cgctcaggat
     8581 gccattgcca tgggcgtttc aggccctctt ttgcgcgcca gcggcgtggc ctacgatatt
     8641 cgcaaagctt tcccctactc gagctatgac gagttcgatt tcaacgtgca gacgcagacg
     8701 gccggcgatt cctacgcgcg gtaccaagtg cgcgtagccg aaattcgaga gagcatcaaa
     8761 atcgtccgcc aagccatgga aaagattccc tcggaaggtc cgttccgcgc ggaggcgccc
     8821 ggcatcattc cgccttcacg cgaggagatg aagacctcca tcgagggttt gatctaccat
     8881 ttcaagattt ttaccgaagg ttttgcgccg ccgcccggcg aggtttacca ggtcatcgaa
     8941 tcgccgcgcg gtgaacttgg cgtcttcatg gtaagcgacg gatcggccaa gccttaccga
     9001 gtgaagttcc gtgcgccgtc gttcgtaaat ttgcaagcgc tgcccaagct gtgtgaggga
     9061 cgcctcttgg ccgatgtggt cgcatgcatc ggcactactg atatcgtcct cggtgaagtg
     9121 gaccgctaac ttccggtgat taaaatttag atgatggaat tgccgccaca actggacgcg
     9181 aagtttaacg agctgatctc gcgctatccc gtgaagcgct cggcgctgat tccgatgatg
     9241 ctatatgcgc aggatcattt cggctatttg ggcgacgcaa ttctcgacga aattgccacc
     9301 cgcctggatc tgaacaagat tcaagtcgaa gaaaccctgg cgtattactc gatgctccgc
     9361 cgcaaacctg ccgggcgcta tcacattcag gtttgcacca atatttcctg catgcttcgc
     9421 ggcggcaatg agctttatca ccacgttcag aagcgcctag gcatcggcaa caaggaagtt
     9481 tccgcgagcg gcacattctc gctcgaggaa gtcgaatgca tgggcgcctg cactggcgcc
     9541 cccgccatgc aggtgaacta tgatttccac gaagatctcg atcccgacaa agtggacacg
     9601 attttcgagc agcttcaaga tggaagagtc ccgcaagcct cgccggttac cgccggctcg
     9661 ttgcacaacc gcttatcagc cgaagttccc gtcatcagca agcgattcgg cattccaaat
     9721 tcgcgcaaga tcgacgtcta tctgaaaaac gaaggctacc aggcgctcga aaaagccctc
     9781 aagcagatga cgcctgagca gattatcgac gaagtaaaaa aatcgaatct acgcggccgc
     9841 ggcggtgcag gttttcctac aggcatgaag tggggtttcg ttcccaaaag caatgacaag
     9901 cccaaataca tcctctgcaa tgccgacgaa agcgagccgg gaacctgcaa ggatcgcccg
     9961 ttgatggaaa tggatccaca tcagttgatc gaaggcatgg tgatcgcagg tcgcgccgtc
    10021 tcggcgaatc gcggcttcat atacattcgc ggcgaatatc gctacatcat tgacatcatg
    10081 gatcgagccc tcgcggaggc ttacgcgcag ggctacctcg gcaaaaatat tcgggacagc
    10141 ggttttgatt tcgaactttg cacgcatacg ggtgccggcg cttatgagtg cggcgaggaa
    10201 tccgcgctga tggaatcgct tgaagggaag cgcggctacc cgcgcattcg ccccccgttt
    10261 cctgctgtcg ttggactcta tggttgcccc accgtaatca ataacgccga gacgcttagc
    10321 accatagcgc cgatcattcg ccagggcggc gactggtacg caagcctggg aactcccaaa
    10381 aacggcggca cacgcctcta ttcgatttcg ggtcacgtga atcgccctgg aatttacgag
    10441 cttcctctgg gctttccctt gcgccgcctg atcgaagaag tagcgggcgg catgcgtgat
    10501 gggaaaaaac tgaaagccgt aatcccgggc ggcagttcct gcccattgct cagcgccgac
    10561 gaaatcgacg tcgccatgga ctacgattcg gttgcgaaaa tcggctccat gctcggttcc
    10621 ggcggcaccg tcataatgga cgaagacact tgcatggtgg atatggctcg ccgcatcatg
    10681 catttctacg cgcatgaatc ctgcggctgg tgcattcctt gtcgcgaggg tacagcctgg
    10741 ctgcgaaaaa tgctcgaccg tttccatgaa ggcgctggtc gcgcggaaga tatcccgttg
    10801 attgacgagc tctcgaaaaa catgctcggc cgcaccttct gtccactcgg cgatgccgcg
    10861 gcgctgccga ccattagtat cgtcaagaaa tggcgcaacg aattcgagga ccacctccac
    10921 ggaaagtgcg cctacaaaag tactgacgtc ctggcggcgg cacactaggg tcgggcagtt
    10981 tgggaatcgg atcccgattt tagaagggaa cacagttagg agtcggatac tggattcgag
    11041 aagagagcgg cagttgaggg gttgagggat aggaccctca ggtccgacga gaagcgtagc
    11101 ccctgaggta agtatcggct tttcattgtg ggacactgca atggcggaac aaaaacaaat
    11161 cgcgttctca atcgacggcc ggccggtgac caccacggcc ggaactttgg taattgaagc
    11221 cgccaaaacc cagggcatcg aggtcccttc gttctgctat tacccgggct actcgctcca
    11281 agccgcttgc cgcatgtgcc tggtcgaagt cgaaaaaatg cctaagctgc aaaccgcctg
    11341 cacgctcgtc gctaccgatg gcatggttgt aaaaaccacc acggatcagg tgcacaaagc
    11401 ccgcaaggac atgattgagt tcctcctgtc gaatcacccc ctcgattgcc cagtgtgcga
    11461 caaaggtggc gagtgcgaat tgcaggatat gaccttccgt tacggcctcg acaagacgcg
    11521 cttcgttgag gaaaagctcc actaccccga ggaaaaatgg tcgcctctcg tttactacga
    11581 cgcgccgcgc tgcattctct gcttccgctg cgtccgtgcg tgcgatgaag gtatggacgt
    11641 gaaagccctg ggcgtcggat cgcgcggcgt gcaatccgta atccttccaa acaagcctgg
    11701 cgaattggct tgcgaagaat gcggcatgtg catcgatatc tgccccgtcg gcgcactcac
    11761 cagcggcacc tatcgctatc aaacgcgccc ctgggagatg acctatttcc ccacgccgtg
    11821 cacccattgc tcgaacggtt gcaaaaccac tctcagcgtt cgcaacaacg aaatcttgcg
    11881 agccaataat cgcgatcatt ccggtttcaa cggcgaattt ctctgcgcca agggccgctt
    11941 cggatttgat ttcacaaatc atcccgagcg aattcgccag ccgctaattc gccgcaatgg
    12001 caaactggaa ccggccagtt gggaagacgc actcgaggaa gtcgcgcgca ggctgaagaa
    12061 ggttcacaaa actcatgggc cgtcatccat cggcgtgatc ggatcgaatc acaccaccaa
    12121 cgaagaaaat tatctcctca atcgcttcac gcgcgtcacc ctcggcacca acaatctcga
    12181 ccaccaccgc actgcggatt acgccgggct cgccgccgca ctcggcccgc gagcgaaaga
    12241 ctcgctggcc actatggcgg atatttacaa tgctcccgct gtcttgctgg ttggcaacga
    12301 cgtcacccag caaaatcctc tagttgcctg gcaaattcgc gccggctttc gccatcacgg
    12361 tttgcggctt tacgtgctaa acggcggccc cagcaagatt catcgccagg cgaagattgc
    12421 gatcgagctg cccgctggca ccgagcgcca ggccgttgag tggcttgcct cagaaaaagg
    12481 ccaactcgag ccgcaggtca ccgaatccct ggtgcggctt aaagccgctc tggaagccga
    12541 gaaagacgtt gtcgtcctct tcggcgcgga tattcagggg tccgcgattc gcgacctcat
    12601 ttctctcgcg gcccgcttcg gcggcgccac gaaattcatg gccctcggcg attattccaa
    12661 ctcccgcggc gctgcggaca tgggtatcct ccctgaccgt ctgcccggct atgcgctgct
    12721 gtccgatgcc ggcgagcgcg cgcgattcgc caaagcatgg ggcggcgaaa tttcggacgc
    12781 gcccggcctc accgccgccg caatgatcga agcggcattg gctggaaaac tgaaagcgct
    12841 gtacgtagtc ggcgcgaatc ccctgaagac ttttgccgcc acccagcctg ataaaatttc
    12901 aggcctcgat ctgctcgtcg tgcaggatat gttcctcacc gaaaccgcac aacgcgcgga
    12961 cgtggttctt cccgccgcct ccagctatga aaaagacggt actctcacca atactgccgg
    13021 cgaagtccag atgactcacc ggtccatcga tccgcaaggt ccgcgcagcg atttcgacct
    13081 gatccgcatc ctttcgcacc aactcggcac gttggggttg ggcgcgccca tccggctgcg
    13141 cacgcccgaa gcagccttcg acgaaatcgg gcagcacgta tccggttaca atctttcgta
    13201 tcccactttg ctcgcgggcg gttcggaaat gtccactccc gcttgcaagg tggccgagcc
    13261 gtcgtacgac gcgcctccgg gcgcggtttt ctcttcacac gattatctct ttaccagcgg
    13321 taccctgggc cgttactgtt ccaggctcac ttccaccaac gaggcgaaag agcggccgtg
    13381 gagttcatct cccagcaccc agtcctggtg gcatcgctag ttaaagttgc cgtcttgttg
    13441 ttcatagtca tgacggcgct ggcgtatctc acctggttcg aacgcaaagt cgtcgcgcgc
    13501 atccaatcgc gctgggggcc gtattacgtc ggcgcccacg gattgctcca gccgctcgcc
    13561 gacggcctga agtttctctt taaagaggac gtcaccccgc ccgcctccga ccgttttgtc
    13621 tacctgcttg cgccttttct ggcgctttcg ctcgcgttaa ccgccctcgc gctcatccca
    13681 ttcgggccgc ccagcgttcg ctttcccaac gggcaatccg ttttcgtcgt ttccgacatc
    13741 gatatcggtt tgctgtttct tttcgccatc acctcgatcg gcgtctatgg cgtcgccctt
    13801 gccggatggt cttcaaacag caaatacccg ctgatgggca gcctgcgcag ctccgcgcag
    13861 atgattagct acgaggtttc gctcaccatg tccgtggtcg gcgtgcttct gctagccgga
    13921 acgctgaatt tcaacgaaat catttcgcgt caaagcggcc acttttttct gcactggaat
    13981 gtgattcaca cgggctacgg atatttcccg caaataatag gcttcctctg ctatctcact
    14041 gccgctatcg cagagaccaa ccgcatcccc ttcgatctac ccgaagcgga aacagaactc
    14101 gttgccggat ttcacaccga atattccagc ttcaaattcg ccatgttttt catggccgaa
    14161 tacgccaaca tgctcaccgt ctcctgcctg gctaccatac ttttctttgg cggctggctc
    14221 tcgcccctgc cggatagctg gcactggcag ctttatttgc ccggggccgg gctgctgctt
    14281 ctgggcctct acactgcgta tgacaccgtt gcaaacatgc gcggcatcgc gcagcttcaa
    14341 tttgccgtgg tcactgcgct ggccctcggt gctggagcca tctgcctgat tcccgtcgtc
    14401 atgcccatcg tccaggggcc cttctggttt acgaccaaag tcctcgtatt catctttttc
    14461 tacgtttggg tccgcggcac gctgcctcgg tttcgctacg accaactgat gtcctttggc
    14521 tggaaacttc ttcttcccgt ttcgctcgca aatctggtca tcaccgcttt tgtcatcatt
    14581 ttgccgaagc tggggcaccc atgagcgtgc cattctttgt gtttttcgcg tgtgctgtgc
    14641 tggccattgc gggagcgctc gtgctcatta tttcgcgcga gccgattcac agcgcgctgg
    14701 ccctgattct ggtcatgatc tcactcgccg tgttgtatct cctcctcggc gctccattca
    14761 tcgccgctgt gcagatcatt gtctatgcgg gcgccatcat ggtcttgttc gttttcgtca
    14821 tcatgcttct gaatgctggc gtggaagagc gcaccaattg gagcaaaatc gcgaaatacg
    14881 cgggcctgcc gctggccgtg gtcctcctgc ttgaaattgc gcactggctg ttccgcgcgc
    14941 cgatcggggc ggcgattgcc aacggcgcgg gcgcgcctgg accggccgcc tccaccgccc
    15001 agctttcttt ggaacttttc cagcaatatc tgttcccatt cgaagcgacc tcgatcctga
    15061 ttctgatcgc gatcctgggc gcccttgtcc tggcgaggaa ggaagaatga gcgtcgttcc
    15121 gatttcctgg tatctcgtgc tgagcgccat cctttttggc ctgggcgttc tggcgttcgt
    15181 tttcaagcga aatatcatca ccattttcat gtccatcgag ctgatgctca acgccgtaaa
    15241 tctcgcattt gtcgccttcg cgcgcgcccg caatcagctt gacggccttg tcttcgtttt
    15301 cttcgtgatc gtcgtcgcgg ctgcggaagc tgccgtcggc ctcgcgatcg taatcatgct
    15361 cgcgcgcaat cgcgagtcgc tcaacgtgga acgcatcaac ctgctcaagt tctgatgccc
    15421 attctcagcc atctctggat tattctcgct ctcccgctcg cgggctccgc gatcaacggc
    15481 ctcttcggca agcgctggcc gcagtccgcc gtcaattccg tcgcggtcgg ctccgtttct
    15541 ttggcctttc tcgccgcagt agagttggcc cgggaatttt cgcatcttgc ccagggccag
    15601 attccgtata tcggaaatta tttcacctgg ataactgccg gatcgtttcg cgccgatttc
    15661 tcgcttcaag tcgaccaact caccatcgtc atggtgctca cagtttcctt cgtaggcctg
    15721 ctcatccaca tctattccac cggctacatg gcccacgagg gcggctacta ccgcttcttc
    15781 agctttctca acctcttcat gtttttcatg ttgacgctgg tgatggccgc aaatctcatc
    15841 gtcatgttcg tcggatggga aggcgtcggc ctgtccagct atttgctgat cggcttctgg
    15901 ttcctgaaga aatccgctgc cgacgctggc aaaaaagcct tcatcaccac gcgcattggc
    15961 gaccttggct tcatggtcgg ccttctgctc gttttttgga ccttcaactc tgagaatttc
    16021 ggtgacattt tccagcaagc gggccacatg gcttcggaaa gcgccaccca cgtgggcgtt
    16081 ctcacagcga tttgccttct gctttttgcc ggagctgtcg gaaagtccgc gcagttgccc
    16141 ctgtacgtct ggctcccgga cgcgatggaa ggccccacgc ccgtcagcgc gctgattcac
    16201 gccgcgacca tggtcaccgc cggcgtgtac atggtggcgc gcatgcaccc cctgttttct
    16261 cgcgcgccca tcgccatgtt cgtcgttgcc gttgtgggcg cggtcaccgc cttttattcc
    16321 gccaccatcg gcctgatgca aaccgacatc aaaaaagtcc tcgcttactc tacggtctcc
    16381 caactgggct acatgttcct ggcctgcggc gtgggcgcct acgccgccgg catcttccac
    16441 ctgatgactc acgctttctt caagggcttg ctcttcctgg ccgcgggcag cgtgattcac
    16501 gctatgggcg gcgagcagga catgacgaaa atgggcggcc tgcgcaaaaa aatccccgtc
    16561 acctattgga cgatgcttat tgccacgctg gccatcgccg gcattcccgg attcgccggc
    16621 ttctttagca aggacgaaat tctcgaagcc gcaggctcag ggcctcacgc caatctttgg
    16681 ctctggctgc tcggcctgat tggcgcgggc cttacctcct tctacatgtt ccgtctcgtt
    16741 tttctcacgt tctttggcaa gccgcgctac gacgaacaca aagtccacgt ccacgaatcg
    16801 cctaagaata tgaccgtgcc cctaatgatt ctggcattct gttcaatttt cggcggctgg
    16861 tttgccgccc ctcacctcgt cggcggcacg gaatacttcg aacactttct gcacccggtc
    16921 ttttcgtcga atgcggtcca ggcagcgacc gagcaagccg gctccctcgc gggcaatcct
    16981 ggcgcacagg gcggcgtggc atccagcccc gcgatggaat tgctccacgc gctcactggg
    17041 tggccagtaa tcgttgccat tctcggtctg ctcgtggcct ggtggttcta catcaaaaat
    17101 cctgacgcgc cgaaacgcct cgcgcaaaaa cttcacggcc tctacatcct tatcttgaat
    17161 aagtattacg tcgacgaaat ttacgccgcc ctgatcattc gtcccttgct ctggatctcc
    17221 accaacgtcc tctggcacgg tgtggaccag ggtgtgatcg acggaatcgt caacggctcg
    17281 gcgcgagtcg ctcgcgaatc gggcggaaaa gtgcgcgaac tgcaatcagg caatacgcgc
    17341 agttacgctg cgtgggtggt gatcggcgcg gtgggtttca cggtgctctt gctcggcata
    17401 ctcggaatgg tggcgcacta gcgtgggaac gcagcctctt ttgctcacgg tgatcacttt
    17461 cctgccgctg ctcagtgtgg cggcgctgct attgctccgc tcggatgacc acctgtggat
    17521 tcgccggatc gcgctggctg cttccgtcgc cgaattcatc atctcgctgc tcctattgcg
    17581 cggctttaat tccggcaccg cctcctatca gttccaggaa tatcacgact ggattggcgc
    17641 cacgattcat taccatttgg gcatcgatgg catcagcctc ttcctcgttc tgctgaccac
    17701 tttcctcacg ccgctggcga ttctctgctc ttggcaatct attcgggaaa acgtcaaagg
    17761 tttcttcatc gcgcttctcg tgatcgagac ggccatgatc ggcgtcttcg tctctctcga
    17821 tctttttctc ttcttcgttt tctgggagct caccctcatc cccatgtatt tcatgatcgg
    17881 catgtgggga cacggccgca agatttacgc cgccgtgaaa ttcattctgt acaccatgct
    17941 cggctccatc ctgatgctgg tcgccattct ttggctctat ggcaaagctg gcacgtttga
    18001 cttgcctcaa atccaacggc tccttcaatc ggggcaaatc gtcttcccgc cgcacaccga
    18061 actgcttctt ttcggcgcgt ttttcctggc gtttgcgatt aaagttccgc tctttccact
    18121 ccacacttgg ctcccggacg cccataccga agcgcctacg gccggatccg taattctcgc
    18181 cggtgtcatg ctgaagctcg gcacctacgg cattctgcgc ttctgcgttc cgctgtttcc
    18241 cgacgccgcg caccgcatgg cccccttgat cgctgttctc gcgattatcg gcatcatcta
    18301 cggcgcgctg gtggccacgg tgcaaactga tttgaagcgc ctcgtcgctt atacatctgt
    18361 cagccacctc ggctttgtag tcctcggcat tttcgcgttc aattccatct ctattcaggg
    18421 cgcgatctac cagatgctca atcacggagt ttccaccggc gcgcttttcc tcggtgtcgg
    18481 catgctttac gaccgccgcc acaccttcga aatcaagcag ttcggcggac tggcaacgcc
    18541 gatgcccgtg ctcatgtcgt tcttcctgtt catctcgctg tcgtctctcg cgctgccgcc
    18601 gctcaatggc ttcatcggcg aatttctgat cctgattgga accttcgccg gccaccacgc
    18661 ctgggccgcg tgggccgcca gcggcaccgt tctctccgcg atttatctgc tctgggccta
    18721 tcagcgcgtg gccctcggcg aagtcaccgt cgaaaaaaat cgcgatctgc ccgacgccag
    18781 caatcgcgag cgcctgattc tcgccaccgt ggccattgtc attatcttca tgggcgttgc
    18841 ctccccgctc ttcacccgcc gcatgcaagc ctcggtaaac aacgtgatgc agcaaatttc
    18901 cccgccgcaa aatgctcagc accgcgtcgc accgccgctg cgcgctgcgg aaacgagtag
    18961 cattttaggt ggccggagct tcagctccga aaaaaagccc gcgtcttaca tgcgcgttaa
    19021 cgcctgggga caggccttgc tctgcttttc ggcaccatgt ctaatctccg actcagcagt
    19081 cacattcgac agagccgcgc cgcgcccgct gatcgcggcc accgctgaaa gacgggtgca
    19141 gcgctgatgt ttctttccgc cgagcagcac gcgaacctgc accggatcgc cccggaaatt
    19201 tatctctgtc ttttcgggct catcatcatg gttgccgacc cgttcgttag cgcggcgcgc
    19261 aagcgcgtcc tcggatgggt gggatttgtc ggcacgctag tcgggctggc agccgtttac
    19321 gaagcttcca tgcataaagg atttgcctac agcaatctga ttagcaacga cgatttcagc
    19381 atctttctgc acgtcatcat tatcggggtt gccgcactcg cgattctcgg ttcgctcgac
    19441 tatctcgaac gtgaaggcat ccagcgcggt gaatactacg ccctgatttt attcgctgct
    19501 gccggaatgg gcatcctcgc gggtgccaac gaactcgtca ccgcattcgt cgggctggaa
    19561 atgagctcga tttcaacgta cgtgctgata ggcttccgcc gccgcgcgct catctccaat
    19621 gaagcctcgc tgaaatattt cctgctcggt tcctttgcca cggcattttt tctctacggc
    19681 attgccatgg tctatggagc cacgggcaca actatcatcg acaaaattca gcccgccctt
    19741 gccgctgttt cctccggcgg acgtctgccc gccctggcca ttctcggcct tggcctgacg
    19801 ttcgtcggcc tgggcttcaa ggtcgtcatt gcgccattcc aaatctacgg cccggacgtc
    19861 tacgaaggcg ctccttcgcc ggtcactgcg cttttggctt cggctccaaa ggccgcggcg
    19921 ttcgcgctca tgctgcgcat tttcgtggtt tcctttggcg ccgcatcgga cacatggttc
    19981 tgggccgtct gggtttccgc cattctcaca atgttcatcg gcaatcttgc ggcgctcgtt
    20041 cagaccaacg tgaaacgtat gctcgcttat tcctcgatcg cgcacgccgg ctacattttg
    20101 gtggcgtttg ctgcgggaac ggcgatcggc gtcgccgctg tgctctttta tctcgccgcg
    20161 tacgtcctga tgaaagttgg cgcattcctg gtggtaacgc atctcggcca acgtggagaa
    20221 aagcgcctca cgcttcagga ctatgccggg ctcggctcga agcagccggt tctggccgcg
    20281 tgcttctctc tgtttctgct ctcgctgctc ggcctgcccg ccacgggagg ctttctcgga
    20341 aagttttttg cgttccaggc ggccctcgat gcatcggacg ctcatggtcg ctggatcatt
    20401 tggctggtgg tgatcgccgc cattaatagc gtgatcggcg cttactatta tttgcgagta
    20461 atgatcacca tgtatttcaa cgagccttca actgactaca cgcccactcg cgtcgctccc
    20521 gcgctcatcg tggccctcgt catcgccgcc gccggcaccc tgtatctcgg catccttccc
    20581 gcccgcgtcg tcgacttcgc caaagtcgcc gccgactccc tctccctcct tcactagatt
    20641 ctggttgtca tcccgcttgc cctgacgcag gaacggaacc acgccgcgtc ttttgcggat
    20701 tcgggcggga ggtctgcttt tgtcgtgcgc tgcctagccc tgagcgtttc ccgcgaaggg
    20761 ttgctgcgcc cggcagagct gttgcttcgt ggagcgtcct attcccaagc ttagctgacc
    20821 gatctgcg
//
LOCUS       DATNDT010000299        13853 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1263521, whole genome shotgun sequence.
ACCESSION   DATNDT010000299 DATNDT010000000
VERSION     DATNDT010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13853
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1263521"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..836
                     /locus_tag="VN788_09645"
     CDS             135..836
                     /locus_tag="VN788_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU20832.1"
                     /translation="MPTTKTAPRIARVRDGGKGNGKAAFDAQAFLGSAGVARKIVEFP
                     RKTIIFTQGVPAKTVMYIQEGGVKLSVVNEVGKEAVVAMLGPGDFFGEGCLAGQPIRI
                     GMATAITLTTVLVIDKDEMVRLLHEEHEFSDRFISYMLTRNLRVEADLVDQLFNSTEK
                     RLARALLLLARYGKEDLTQRVLPRVSQETLAETIGTTRSRVNLFMNKFKKMGFIKYNG
                     GLQINSSLLSVVLHE"
     gene            complement(880..2217)
                     /locus_tag="VN788_09650"
     CDS             complement(880..2217)
                     /locus_tag="VN788_09650"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXU20833.1"
                     /translation="MQNDLQLDLAILPPEGTASHRHRPIRVLFVHGDTEVVDNCLREL
                     KKALFIVTADVVLNLTGCMQQLRSQTYDVVLAEYPSRNWEGSQALQLLHQTVQETPLL
                     FVTTIPGGKSLAELAGHGAFDYVEQEHMAHLPMAVRRAVNEKTLRAELEQAEKALQHS
                     QSRYRALVDNPAYGICQCDAKGKFLDVNEALLTMLGYATQEELLAADGESEIVLDLKQ
                     GKPLPGSSPESARIESVEIEWKRRNGTILKARLSGRGVFSELGDFAGYEIIVVDITEQ
                     RVLEDHLRRQVLSDSLTGLANHGRLFEVLHAEIERSKRTEREFSLLLLDLDGLKEIND
                     RFGHLTGDRALCRLAQILADCSRSVDTAARQGGDEFALVLPETSSASATLVAGRIRNL
                     LVKDTEEPALSVSVGVACYPKDAATIGTLLYAADGALYAMKNKRSRAARVGLS"
     gene            2547..3113
                     /locus_tag="VN788_09655"
     CDS             2547..3113
                     /locus_tag="VN788_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20834.1"
                     /translation="MQKQFILLGLLLASHLSTSAQTFTPETAPAQDRIAETVPIVRTG
                     PTMLLTASLLPPENPGKFAVNVNYPFEMVYERDQVLESLESLSMMRQVKTLFLTESSL
                     PLIQLWGGRLRLDGFTSTLHMQNVQLGPSASGGSRDFRPARQSYRGGPRSVDLYGLSL
                     TFHFGRDAQIGRPTQIWRRVARIVRAAR"
     gene            3303..4067
                     /locus_tag="VN788_09660"
     CDS             3303..4067
                     /locus_tag="VN788_09660"
                     /inference="COORDINATES: protein motif:HMM:NF028441.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HXU20835.1"
                     /translation="MISSGQFARLMFIYFAAAICTSAQTHSVVPTSAHIVARMAQAQA
                     ENRDNVHPYIVTRDYKLFGHESSLESKSHVIAEITVEPSGSKKYTIESAEGSGSGERV
                     VRKMLEGEVAFAKDSGSTDITRKNYDFVLVGEDDLNGQPCYVLQLLPRRKSKDLLRGT
                     IWVDANTYLPRRVDGEPVKSPSWWLRDVHVVLVYGDVGSMWLLTSSKATANVRIIGRS
                     TMVWHDVSYQMGELTSGASVAQTTVPADEAIAEDQW"
     gene            4168..4443
                     /locus_tag="VN788_09665"
     CDS             4168..4443
                     /locus_tag="VN788_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20836.1"
                     /translation="MGLEKLKGRGRLVPTLATGLEYQVNYEIHFPILEREPLRRLTPN
                     HMCSSKCFVRSAHHHRIPDGPYFLHINAGQVHQVKSIDGKWHYLATR"
     gene            complement(4576..6216)
                     /locus_tag="VN788_09670"
     CDS             complement(4576..6216)
                     /locus_tag="VN788_09670"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXU20837.1"
                     /translation="MKISAVLLAALMCLSASTLAHAQIATSRKSNQTPTYRASVVRTT
                     RAVKYEHRSGSTKINFRGTDLMPTASGEAQVESKRGAIKIEAEFGGLDRPTTFGNEYL
                     TYVLWAISPEGRSVNVGEVLVGNNHRSKLAVTTDLQAFALIVTAEPYYAVRRPSNVVV
                     LENEVRADTAGTSEEVDAKYELIDRGGYIPTGYKFDPVVLNAKLPLEFFEARNAVRIA
                     KSAGAEENASSSYENAVGQMKRADDLATTKHVDKKSLISMSREVVQTADDAREIAVKR
                     MDADRVIAESNAAADRVADADAQSKQAMADRMSAEAATADADRRRAEADRASAAAREQ
                     QLDAQAESDRNRAAAVDANKATADAQQGQRDAEAESERNKAAAAKSDQEVQQAVRDRE
                     ELRAKLLQQFNAILATRDTARGLVVNLSDVLFDTGKYTLRPLAREKLAKISGIVLAYP
                     DLKLTIEGNTDSVGSDAMNQVLSEKRAGSVMEYLAAQNIPAASMTSQGFGKSQPVASN
                     ATPEGRQQNRRVELIVSGEVIGTTIGAASQSLPMNARR"
     gene            complement(6795..6947)
                     /locus_tag="VN788_09675"
     CDS             complement(6795..6947)
                     /locus_tag="VN788_09675"
                     /inference="COORDINATES: protein motif:HMM:NF039824.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXU20838.1"
                     /translation="MFGIIAAVLIILWLLGFFAFHVTTAFIHIALVVGLVLLVLHFMR
                     ARPAIV"
     gene            9955..11136
                     /locus_tag="VN788_09680"
     CDS             9955..11136
                     /locus_tag="VN788_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXU20839.1"
                     /translation="MARRGFQRGSLFRRGTRKKVWVARWWEDVIHTDGTFGRLRRSEV
                     IGTVADYPTRSRAMQVLSQRLGSINSGKTRPQSVRTFGDFAKDDWMPVVLPTLKYATQ
                     KHYKYMLDVHLVPAFGKRQLRELSREELQSFLSQKLNGGLSWAMVHHFKCGLSKILSA
                     AEEWGYITENVAQKTKLPRRQHGVERAVLTPVQVRNLAAGLNEPARSVTSLLVLTGLR
                     VGELLALRWGSIDLNARLLRVCETVYDGHFDQPKTKRSARTIPIGTETAEILATLRPA
                     AVDPKALVFATREGLPLERWNLLRKHLKPAAKKLGLPGVTWHLLRHSHATMLDSVGTP
                     LGTMQSLLGHSTPEITREIYLHAVPEEQRRAVESVERLVFGLKSTQVQAPTQTAPARW
                     N"
     gene            complement(11152..11228)
                     /locus_tag="VN788_09685"
     tRNA            complement(11152..11228)
                     /locus_tag="VN788_09685"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11192..11194),aa:Pro,seq:tgg)
     gene            11523..12221
                     /locus_tag="VN788_09690"
     CDS             11523..12221
                     /locus_tag="VN788_09690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU20840.1"
                     /translation="MAVETQEAVLRKTQLFARLTESEMRMLCARVSKKHFGRGETLFS
                     EGDQCHGLFVVATGRIRIFKLSPSGREQVLAVEGPGSSFAELPVFDGGVYPASASALE
                     DAEVLFISRKDFQNYCREHPEVALKVLAVVGSRLRRLVGIIEDLSFTTVRQRLISLLL
                     RLAQGSGTATKQGVRVELSTTHQDLAAELGTVRELVSRNLSRLQAEGLLEVDGRTIIV
                     KDLAGLKREQVASE"
     gene            12323..13060
                     /gene="ric"
                     /locus_tag="VN788_09695"
     CDS             12323..13060
                     /gene="ric"
                     /locus_tag="VN788_09695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein"
                     /protein_id="HXU20841.1"
                     /translation="MNVNVETKMKDIALSNPTARQVLEDAGLDYCCGGGKSLHEACLH
                     ANVSPEEILNRLRENAKDTGPEDLNWMAAPLNELTRHIREKHHRYVRDAIPRTRALSD
                     KVTAKHGSNHSELADIGKVFAEVGREMIMHMQKEEQILFPYIDALERAVNAHGSVEPP
                     FFQTVRNPIQAMMKEHDAAGDLVRQIHNLTSGYTPPANACTSFKALYEALREFEADLH
                     EHVHLENNVLFPRAVELEASVTHEVRD"
     gene            13097..>13853
                     /locus_tag="VN788_09700"
     CDS             13097..>13853
                     /locus_tag="VN788_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20842.1"
                     /translation="MVWFDAAHRAVHYPPDRRFRIWIRPAILIGTGIAILSVIVAAWI
                     EVAVAGMPRVPAVPQIYPNNFAGPHGFPAWVRYCHFFNFLFVMMLIRSGLSILVDHPR
                     LYFNDGCTPGSEWIRFTPLKVPRDRIWTAKDDARYISPLIGTPGYRHTIGIARVWHFI
                     DVHGFIITGIIFIAMLFDTEQWRRIVPTSPLVLFQAWNTFVHYATFHLPPEPNGFYGY
                     NALQQIAYFTVVFVFGPLAIATGIAMSPAVVNRF"
BASE COUNT         3079 a         3816 c         3826 g         3132 t
ORIGIN      
        1 cgtgattggt ttggaacaag ccaggaagcg tttgaatgac ctcgccgatg ttgaatccgc
       61 cgagtacttg atctacgatt cccacatggc gacgttcatc aagtcggctg ccggcccaac
      121 gagcgagttc cacgatgcca acaactaaga ccgctccgag aatcgctagg gttcgcgatg
      181 gtgggaaagg aaatgggaag gcggcatttg acgctcaggc tttcctcggg tcggcgggtg
      241 tggctagaaa gatcgttgag tttccacgaa agacaatcat cttcacacag ggggttcccg
      301 ccaagaccgt catgtacatt caagaagggg gcgtgaagct atccgtggtc aatgaggttg
      361 gcaaagaagc tgtcgtggcg atgctgggcc ctggcgactt ttttggagaa gggtgcctcg
      421 caggacagcc tattcgaatt gggatggcga ccgcgattac actgaccacg gtgcttgtga
      481 ttgacaagga tgagatggtg cgactgctcc atgaggagca cgaattctcg gatcgcttta
      541 tttcttacat gctcaccagg aacctccgag tcgaggcgga tttggtcgat caactcttta
      601 attctactga gaaacggttg gcgcgagccc ttctactgct tgctcgatac ggaaaggaag
      661 atctaactca aagggttctt ccaagggtat cgcaagagac tctagcggaa acgatcggta
      721 ctacgcgctc cagagttaat ctcttcatga ataaattcaa gaagatgggt ttcattaaat
      781 acaatggcgg ccttcaaatc aatagctccc tcctaagtgt tgtcctccat gaataatcaa
      841 agcgcgtaac tctcgttggg aaaatgacac attgtggatt cagcttagtc cgacccgagc
      901 ggctcttgac cgtttgtttt tcatcgcgta gagggcccca tccgctgcgt ataatagggt
      961 tccaatcgtc gcagcatcct tcggataaca ggcaactccc acgctaacgg aaagcgccgg
     1021 ctcttcggta tccttcacaa gaagattgcg tattcgtcct gccaccaatg tggccgaagc
     1081 tgagcttgtc tccggcagga ccaaggcaaa ctcgtctccg ccttgccgcg ctgcagtatc
     1141 caccgaccga ctgcagtcag ccaggatctg cgccaacctg cagagcgccc ggtcacccgt
     1201 cagatgaccg aatcgatcgt tgatctcctt caatccgtca agatcgagta acagaagcga
     1261 gaattcccgt tcagtgcgct tggatcgctc gatctccgca tgcaacacct cgaaaagccg
     1321 gccatgattg gccaaaccgg tcagcgaatc gctcaaaacc tgacggcgca gatgatcctc
     1381 aagaacgcgc tgttctgtaa tgtcgacaac aatgatttca tagccagcaa aatccccaag
     1441 ctcgctaaag accccgcggc cgctgagcct ggctttcaga atggtgccat tccttcgctt
     1501 ccactcgatt tcgactgact cgattcgggc tgactcgggc gagcttccgg gcaatggctt
     1561 cccctgctta agatcgagga cgatctcaga ctcaccgtcc gccgccaaaa gttcttcttg
     1621 tgtggcgtag ccaagcatgg tcaagagggc ctcgttgaca tcgagaaact ttcccttcgc
     1681 atcgcactgg catattccgt acgcaggatt gtccaccagc gcgcgatacc gcgattgcga
     1741 gtgctgcaag gccttttctg cctgttctag ctcggcccgc agggtctttt cgttcacggc
     1801 tcggcgaaca gccatcggca agtgagccat atgctcttgt tccacgtaat cgaacgcgcc
     1861 atggcccgct agctccgcca gggatttacc tcccgggatg gtggtcacaa agagaagcgg
     1921 cgtctcctgc acggtctgat gtaagagctg caaagcctga gagccttccc agttccggct
     1981 cgggtactca gctagaacca catcataggt ttgggagcgc agctgttgca tgcaccctgt
     2041 taagttcaaa acgacatcgg cggtgacaat gaacagcgct ttcttcagct ctcgcaggca
     2101 gttgtctact acctctgtat cgccgtggac gaaaaggacg cggataggcc ggtgtctgtg
     2161 ggaagcggtg ccttccggcg ggaggatcgc gagatcaagc tgcaaatcat tctgcatcag
     2221 gtttgaactc ctagatggga tcgcacgccg ctcatggata tttccgcgag cggctaagag
     2281 tgttccactt cccgctcact ataagccggt aagaaaacgg attctgtctc gtttcgaacg
     2341 cttttgacat gacgcaaggc cagcgaaacg ctgggcagcg aactgggaac gccagactgg
     2401 ggaaagccaa gcgatcgggt gcctgtgcgt aagaaaagga atctgacgtg tgcgaattag
     2461 gacagaccgg cgccactcag atccagtaat gtcacttcgt aagtagcgct tatcttcccc
     2521 gctttaccgc gctatcaagg cagggaatgc aaaaacagtt cattctgctc ggcttgcttc
     2581 tggctagtca tctgtcaacg tcagcgcaaa ctttcactcc agagactgcc ccggcgcaag
     2641 accggattgc ggagactgtg cctatagttc gaacgggacc gacaatgttg ctcacggcct
     2701 ctttgctgcc gcctgaaaat cccggaaaat tcgctgtcaa tgtcaactac ccgttcgaga
     2761 tggtttacga gcgggaccag gtcctggaga gtctggaaag cttgtcgatg atgcgccagg
     2821 ttaagacttt gtttcttacg gagtcgagcc tgccgcttat ccaactttgg ggcggacgtt
     2881 tgcggctcga tggctttacg agcacgctcc acatgcaaaa tgtgcagctt ggtccttcag
     2941 cctcgggggg ctcgcgggac tttcgtcccg cgcgccagag ttatcgcggc gggcctcgtt
     3001 cggtcgatct ctacggcctc agcctgacct ttcactttgg acgagacgcg caaatcggac
     3061 gtcccacgca gatatggagg cgcgtcgcgc gaattgtccg tgccgctcga tagtctgacg
     3121 agatccggcg ctaatcgaga gctcccaaag ttggggtttt ccttcacggc aacgccattt
     3181 ccggacgccg ttcttatgaa aacaagaaca ggctgcagca cctgaaatac agaggtgtgc
     3241 gcaagagaac agaaatcgac tttcccccgg ctctaggatg gaatcgaagg cggtgtttca
     3301 acgtgatttc cagcggccaa ttcgcgcgct tgatgttcat ctacttcgca gctgcgatat
     3361 gcacttcggc tcagacacac agcgtggttc caacaagcgc acacatcgtt gctcggatgg
     3421 cgcaggctca ggccgaaaac cgcgataacg tacacccata tatcgtcacg cgcgactaca
     3481 aactattcgg ccatgagagt tctcttgagt ccaagtccca tgtgattgcc gagattaccg
     3541 ttgaaccatc gggctccaag aaatacacaa tcgaaagtgc cgagggatcc ggatcgggag
     3601 agagagttgt tcgcaaaatg ctcgaagggg aggtagcctt tgcaaaggat tctggctcga
     3661 ccgacatcac gcgcaaaaat tatgatttcg tgctggtcgg tgaggatgac ctgaacggtc
     3721 agccctgcta tgtgctccag ctacttccca ggcgaaagtc caaggacttg ctgcggggaa
     3781 ctatttgggt ggatgccaat acctacctac ctcgtcgcgt cgatggggaa cctgtaaaaa
     3841 gtccgtcctg gtggttgagg gatgtgcacg ttgtcttagt ctatggcgat gtcggctcca
     3901 tgtggctgct gacgtcttca aaagcaacag ccaacgttcg gataatcggg cgatccacca
     3961 tggtttggca tgatgtgagc tatcaaatgg gcgagctcac ttctggagct tccgtggcac
     4021 agacgacggt tccagcggat gaggcaatcg ccgaggacca gtggtgatgt ggcagtttct
     4081 catggcggag ctcgccgtcg caaacgagac tccggcccac tcgaacatac gacatgatca
     4141 ggctggaatg ggccgcggag gaaaaacatg gggcttgaaa agctgaaggg acgcggacgg
     4201 ttagtgccga cactggcgac gggactcgag tatcaggtga attatgaaat ccattttcct
     4261 attctcgaac gagagcctct gcgccggctg acgccaaatc acatgtgctc ctcgaaatgt
     4321 tttgtgcgtt ctgcccatca tcaccggatt ccagatggac cttattttct ccatatcaat
     4381 gcgggccaag tccatcaagt aaagtcaatt gacggcaagt ggcattacct tgcgacccgc
     4441 taacaggaga attccggcca cgactaggcc acgcggaatc ctccttactg gaaaatcaaa
     4501 gtgattcacg aatccaccca cgatattacg caggccccgg gatttccggg gcctgccaat
     4561 catgctgtcg tgcgttcagc ggcgtgcgtt catcgggaga ctctgagatg cggctccgat
     4621 tgttgtgccg attacttcgc ctgatacgat cagttccacg cggcggtttt gttgccgccc
     4681 ttctggcgta gcgttggatg caacgggttg actcttgccg aaaccttgcg acgtcatgga
     4741 cgctgccggt atgttttgcg ccgccaggta ctccatcacc gaaccggctc gtttttccga
     4801 aagtacctga ttcatggcat cgctgccgac gctgtccgta ttgccctcga ttgtcaactt
     4861 gagatctggg taggcaagaa cgatccccga tattttggcc agcttctcgc gtgccagcgg
     4921 gcgaagcgtg tacttgccag tatcgaaaag tacgtccgac aggttcacga caagaccgcg
     4981 agcggtatcg cgagtagcaa ggatggcgtt gaattgttgc agcaatttgg cgcgaagttc
     5041 ttcacgatcg cgcacagctt gctggacctc ctggtccgac tttgcggccg cggctttatt
     5101 tcgttccgat tccgcctcgg catctctttg gccttgctgg gcatccgccg tggctttatt
     5161 ggcgtcaacg gcagcagccc gattgcgatc tgattccgcc tgggcatcta gttgttgttc
     5221 gcgtgccgct gcactcgctc ggtcggcctc tgcacgtcgg cgatccgcat cggcagttgc
     5281 cgcttccgcg ctcattcgat ctgccatggc ttgtttagat tgagcatcgg catccgccac
     5341 tcgatccgcc gccgcgttgc tctcagcgat cacgcggtcc gcatccatcc gcttcacggc
     5401 gatttcccgg gcgtcgtccg ccgtctggac aacctcgcgg gacatagaaa tgagcgactt
     5461 cttgtccaca tgcttcgtgg ttgctaaatc gtcggctcgc ttcatctgac caacagcatt
     5521 ttcatagctg gagcttgcgt tctcttcggc cccggccgac ttggcgatac gcaccgcatt
     5581 gcgcgcttcg aagaattcga gcggcaattt ggcgttcaag acgacgggat cgaacttgta
     5641 gccggtggga atgtagccgc cacgatcaat cagctcgtac ttggcatcca cctcttcgct
     5701 cgtgccggcc gtgtccgccc taacttcatt ttcgaggacc acgacgttgc taggacgcct
     5761 cacagcataa tagggctcag cagtcacgat cagggcaaac gcctggaggt ccgttgtgac
     5821 agcaagcttg ctgcggtggt tattgccaac caacacttcg cccacattaa cggatcgacc
     5881 ttcgggagaa atcgcccata ggacataggt gagatactcg ttaccaaaag tagttggtct
     5941 atcgaggcca ccgaactcag cttcgatttt tatagcacca cgcttgctct ctacctgcgc
     6001 ctctccggac gcagttggca tcaagtcggt accccggaaa tttatctttg tgctgccact
     6061 gcggtgctca tatttgactg cacgtgtcgt ccggactacg ctcgctcgat atgtgggcgt
     6121 ctgattggat ttgcgggagg ttgcgatttg ggcatgagct agcgtcgatg cgctcaaaca
     6181 catgagggct gccaagagta ctgctgaaat cttcatggtg agtctccttg aaaagctgcg
     6241 tggcgctaag tatcggtgtt ttgcgattgt gaatactgtc cgttaaagtg ccttttatct
     6301 tgaagtaccg agctcggcag tagcgcgttt tatcaggatc tgggcgaccc ttgggatttt
     6361 cgctgcagga acgagtgagt ctcgctgcac agttcggagg ccaacagtat aattccggga
     6421 atggcgctag ctcctcttgc aatgaaagta cgtccttcga gtttcatttc tcgctctaac
     6481 tcatggtggg atgggtcgaa aaacagaatt ttcagggtgg gcttgcaccg aatgcgcgtg
     6541 ggcgttcaac gctctagggc cgctcgttgg caaatcaatt gacgaaatga agatgcccta
     6601 agaacagcag cgcgacaagg agttcacgtc gcatgtctgt gccgagcacc cgagagccac
     6661 aaaagattcc cgttagccaa atcacccgcg ggctaaatgc cgtgtaagcc tcgtgttcgt
     6721 gcaaagctgt tttggtccgc ggcaccctaa ctattggcac cctcgggcgg ctctccgcga
     6781 atctggcgct cgttctacac gattgctggc ctcgctctca tgaaatgtaa aacaagtaac
     6841 actaggccca caactaaagc aatgtgaatg aaagcagtgg ttacgtgaaa ggcaaagaat
     6901 cccagcaacc aaagaatgat tagaaccgct gcgattatgc caaacatgtt ttcacctcct
     6961 tgcgataggg tgcgagagca tccgcgaatc tcgatctgtc gaaccgcagt agtagctcct
     7021 cgtggtctgg aaactgcaca aaccagatta gtcacagaaa agacgctaat ctgtctcgtt
     7081 gcgaacactt tcgcattatg tcttatcgtt caaggtgatg ccgctgagaa cgtctatgta
     7141 cttgcaagaa ggcggtgtga agctcgccgt agtcaatgaa gtcgggaaag ggtcggtcgt
     7201 cgccgttccc ggaccgagtg accttttggg tgagggctgc ctggcgggtt agtctgttcg
     7261 cacggggcag agcactgtgc cagcgccggg ccttcgtcca ttggccccta atcgaaattc
     7321 cgaggccgtg cgcgggcctg acgagctcac tcattgccat ccccatattc agcctgccga
     7381 ccctggcagc gagacctcga aaacgcgaaa ggaatccatc aactgtccgg cgaactcttg
     7441 ccagtgcttc ttcgtcgtgg atgccccaga aatccagcag ccgcccggtc ttgcagttgt
     7501 aagcgaatct ttgaccgcaa tccaagcaag tgatgtaggt tccccgacta gagccagttc
     7561 ggtgtccgga taagctgaac gagatcggct cgcgcagcgt tatcggagga cccttgcgcc
     7621 gatgggggca aagaaaaaca agacgaagca gcgtccgcaa aagaagcggg gctgcgaggg
     7681 cgacactcca cgaaggcacc gcacggaaaa gtgatctttt cgttttgaac ctccttctcg
     7741 ctgccgagcg atattgaact gcagagccct cgagtctata tgctcgggtc agttttttgt
     7801 gccgtaaagg ttgcatctga gcgcctcagc aggaggctag ccgcctcgaa aattcgtgtc
     7861 tgtaccgttg tgaacacttc gtaacagatt tgcctgtttg tctctatacc gtctttctgg
     7921 cctccccgcc cggcgcgctc gccgtgttgt gggcgaccta caggtaccgc tatccggaaa
     7981 gcccggcatc aagcgaggtg ttcaatcgcc cgctagccga caagatctct gacgtgcgct
     8041 cgcctgccta tcgtgcagtg ctccacggga tgcgcctgac ccacatcgtt cccgcaccta
     8101 catctggagt ctcccggata cggtccgggt gggtctggaa ggccgcatca ttcccatcac
     8161 cgcgcttgga catgcgtacg tcgaaagggc ccgaaatgac tacttcccag cgatcgtcgc
     8221 ttacagttgc caatcgggct gagcaccctc atcctgatcg gcctttttgc gctcgccgca
     8281 cctcgcccgg cgctttcacg accgtgcttg ccgccgtggc cttgttaatg ttggtgttaa
     8341 tttcgcgtct gcttgcgccg gcatccgcca tgccctacgc gttgtggccc ttctcgcgat
     8401 cataggaggc gtcgccatgc agtttgcgtt ctctcgaact ccaccccatg gaaggtcgca
     8461 gctggagctg cccttgcgat tgccgctgcg cctgctttgc cggcaatgcg cccatgggaa
     8521 tacttcaacg aattcgtagg cggcccgatt cgcgcctatc tgtacttcag cgatgagggc
     8581 gtcgatcagc gcgtcaagga acttgccgcc tattatttat ttttatggac cgataagcga
     8641 agtcgaagaa agagcgcata gcattgactg gctgggacgc gacccggggc gcgaccaatc
     8701 cagacttgaa tccgcacgtt ttccggcacg tgatcgttga cgcgcgcttt ctcgggaagc
     8761 atccattctg ggacaatgtg gcactgcaaa gcgcgactcc ggctgctcga tttagcttag
     8821 acagacaggg gtgaacacag gaacactcac agtgttcacc cttaaagata ggcgttccgt
     8881 tcacctaggc cccttcggca aggctccatc gaagaataca ctgcggaaat cagctctgca
     8941 gaaagaccgc ggacgaaaac ctcggtttgg gcgggagcga ccctctgttt tgggcgaaaa
     9001 tgccgcccaa acgtaatgca tggggtcgct cccgcccaaa ccgatcccgt cgtgcgccgt
     9061 gtaatcggcg cacgtctaag gatgtccgcg tgagttgcca caagctccaa taaattccat
     9121 ctcgtcggcg cgacccgaaa aaatctattt tgaggcgcgt atattttctt tgggactttt
     9181 tccgacttct tgcccattag tataaataga gggccactct tcgtcggcgg acgttgacgc
     9241 aaccttcaga gaaaccgcat cccatcagca tttccgacag ttccttctgg ttccccacaa
     9301 tcgaaaggag acgcgtatgg ccattctcaa agcgccgccc gctcaaccca agaacgaaac
     9361 tctacaactg cgtgtatcgc aggatttaaa gttccggcta atgcgttacg aggagttcct
     9421 ccacgcgaac gcctgatacg tcgtaaccgc ggccctcgaa cgcctattcc gcaaggatgc
     9481 agaattccag gaatttcttg cgtcgtacaa gccgggaaaa tcaccggacc acagtgagga
     9541 cgaaaggccc atcaagaacg ctgtaaattc actcgccaaa taacgcgcaa attcgttctc
     9601 taaaaccaca gttaaaaccc tcgcagaccc tagcacattt tccgcattac aaaacgcctc
     9661 tgaaggagcc gccgcatgaa cacggaatcg aagtgtgagt ttttgaccgt tcagcaggtt
     9721 gccgacctac tacaagtccc cgtgtcctgg gtgtacgggc gctacgcaag cgttcgctcg
     9781 aaaaattgcc cggatatcgc ctcggaaaat actggcgttt cgacaaggag gaggtcttgg
     9841 tgtggctggc gcaacagcgt gagggccgaa aggcggcttg acatgcgcac tacactgaga
     9901 aacgacgcag aacaagctgg gcgggccatc gggactccaa cgaagggaga accgatggct
     9961 cgaagaggtt ttcagcgagg ctcgcttttc cggcgtggca ctcgtaagaa agtctgggtg
    10021 gcgcgctggt gggaggatgt aattcacacg gacggcacgt ttggcagatt gcggcgatcc
    10081 gaagtgatcg gcaccgtcgc tgactatccg acacgcagcc gtgcgatgca ggttttgtct
    10141 cagaggttag ggtcgatcaa cagcggcaag acgaggccac agtccgtccg aactttcggc
    10201 gatttcgcca aagacgattg gatgcccgtg gttttgccga ctctcaagta cgcaacacag
    10261 aagcactaca agtacatgct cgacgtgcat ctcgttccgg cattcggcaa gcggcaactt
    10321 cgcgagctct cgcgcgagga gttgcagagc ttccttagcc aaaagctgaa cggcggattg
    10381 tcatgggcga tggtccacca cttcaaatgt ggcctgagca aaattctcag tgcggccgag
    10441 gaatggggct acatcacgga gaacgttgct cagaaaacga aactcccgcg acggcagcat
    10501 ggagtggagc gggcggtcct cacaccagtc caggtgcgaa acctcgccgc cggtttgaat
    10561 gagccggcac gctcggtcac gtcgctgcta gtgctgactg gcttgcgcgt tggagagttg
    10621 ctcgcgcttc gttggggaag tatcgatctg aatgctcgtt tgctccgtgt ttgcgaaacg
    10681 gtgtacgacg ggcatttcga tcagcccaaa accaagcgca gcgcaagaac catcccaatt
    10741 ggaacggaga cggcagaaat tctcgccacg ctccgtcccg ccgctgttga tccgaaagcc
    10801 ttggtattcg ccacgcgcga aggactgcca ttagagcgat ggaatctctt gcgcaagcac
    10861 ctgaagcctg ccgcgaagaa attagggctt cctggtgtca cgtggcacct tctgaggcat
    10921 agccacgcga cgatgctgga ctccgtgggc acgccgctag gaacgatgca atcgctcctc
    10981 gggcattcta cgccggagat cacgcgagag atttatttgc acgcggtccc ggaagaacag
    11041 cgtcgcgcgg tagaaagtgt cgagagactt gtatttggac tcaaatcgac ccaagttcag
    11101 gctccgacgc agacagcacc ggcaagatgg aattgaaaac aaagaagtaa gtggtcgggg
    11161 cgtggagatt tgaactccag acctcctgcg cccaaggcag gcgcgctacc aggctgcgct
    11221 acgccccgat atggagtgcg tattatacgg cacttgccaa cgcggatgct gctccaacca
    11281 gccaagttga ccttcaactg tgcgcaaact atgccaaata gatttcattg aaccatcatc
    11341 gtgcaaaatc cagactcgaa gctgcctgat ttcttctttg ggagccttgc tagtgaggag
    11401 catgcgtgct tagcggctta aaaacgtgcc agaatgcggc ttcaatttga cacaaacgct
    11461 gccactcggg ggcagatgcc atgcgcactg taatatgact ggggggaagc gagtttaggc
    11521 aaatggcggt cgagacccaa gaagccgtgc tccgaaagac gcagctattc gcaagactga
    11581 ccgaaagcga gatgcgcatg ttgtgcgcac gcgtgagcaa aaagcatttt ggtcgcggcg
    11641 agacgctatt tagcgaaggc gaccaatgcc atgggctgtt tgtggtagcc accggcagaa
    11701 ttcggatttt caaattgtcc ccgtccggcc gcgaacaggt tttagcagtc gaaggaccgg
    11761 ggagctcttt tgccgagctt ccggttttcg acggcggcgt gtatccggcc tcggcatccg
    11821 ccttagaaga tgccgaagtg ctgttcatct cacgcaagga tttccaaaat tactgtcgcg
    11881 agcatcctga ggtcgcactc aaagttcttg cggttgtcgg ttcgcgtctg cgccgtctag
    11941 tcggcatcat cgaagatctg tcctttacaa ctgtgaggca gcggttgatt tcgcttttgt
    12001 tgcgcctagc tcaagggagc ggaacggcta caaaacaagg tgtgcgcgtg gaattatcga
    12061 caacccacca agacttggcg gcggaattgg gaactgtgcg ggaacttgtc tcccggaact
    12121 tgagtcggct ccaggctgaa gggcttctgg aggtagacgg ccgcacgatc attgtaaaag
    12181 acctcgcggg actcaagcgt gaacaggtcg cctcggaata atccacagag cagcgtctcg
    12241 tcgtgaaaac cagggcctag tgactttagt cactggcaac aaatccttta gagtagacaa
    12301 tgttgacgtg atggaggtca ccatgaacgt caacgttgaa actaaaatga aggacatcgc
    12361 actttcgaat ccgacagcaa ggcaagtgct tgaggacgca gggctggatt attgctgtgg
    12421 cggcggtaag tctcttcatg aggcgtgcct tcatgcaaat gtctcgccgg aggaaatatt
    12481 gaaccgcttg cgagaaaacg ctaaagatac cggacctgag gacctgaact ggatggccgc
    12541 gccattgaac gaactgacgc gccatatccg tgagaagcac caccggtatg ttcgcgacgc
    12601 gattcctcgc acgcgggcgt tgtcggacaa ggtcacggca aaacacggaa gcaatcactc
    12661 tgagctagcg gatattggga aggtcttcgc ggaagttggg cgagagatga ttatgcacat
    12721 gcagaaggag gagcaaattc tgtttccgta catagacgca ttagagcggg ctgtgaatgc
    12781 gcatggttcg gttgaaccgc cgttctttca gactgtaagg aatcccattc aggccatgat
    12841 gaaggagcac gacgcggcgg gagatctagt caggcaaatt cacaatctaa cttcggggta
    12901 cacgccgccg gcaaatgcgt gcactagctt caaagcactg tacgaagctc tccgagaatt
    12961 cgaggccgac cttcacgagc acgtacatct tgaaaataat gttcttttcc ctcgtgcggt
    13021 tgagttggaa gcctctgtta cacatgaagt gcgtgactaa cggattagac tgaacggcag
    13081 ttcgtggagg ccagggatgg tttggttcga tgccgcgcac agagctgttc attatcctcc
    13141 tgatcgccgc ttccgcattt ggattcgacc tgccatcttg atcgggaccg gtatcgccat
    13201 cctctccgta attgtggccg cttggatcga ggtggcagta gccggaatgc ctcgggttcc
    13261 ggccgttccg cagatatatc cgaacaactt cgctgggccg cacggctttc ctgcatgggt
    13321 tcgctactgc cacttcttca attttctttt tgtgatgatg ctgattcgga gcgggctttc
    13381 gatcctagtg gaccatcctc gcctctattt caatgatggc tgcacgccgg gcagcgaatg
    13441 gatccggttt acgccattga aagttccacg cgaccgcatc tggaccgcaa aggatgatgc
    13501 gcgatacatc tcgccgctga ttggcacgcc gggctaccgg cacaccatcg gcattgcgcg
    13561 cgtgtggcat ttcattgatg tacacggctt tatcatcacc ggcatcatct tcattgccat
    13621 gctgttcgac accgaacagt ggcggcgcat cgtgccaaca tcgccgctcg tacttttcca
    13681 agcatggaac acgttcgtgc attacgcgac attccacctg ccgccggagc cgaatgggtt
    13741 ttatggctat aacgcattgc aacagatcgc ctatttcacg gtggtgtttg tattcggtcc
    13801 gcttgcgatt gctaccggca tagcgatgtc tccagcggtg gtcaaccggt ttc
//
LOCUS       DATNDT010000300         5600 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2941993, whole genome shotgun sequence.
ACCESSION   DATNDT010000300 DATNDT010000000
VERSION     DATNDT010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5600
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2941993"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..485)
                     /locus_tag="VN788_09705"
     CDS             complement(<1..485)
                     /locus_tag="VN788_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HXU20843.1"
                     /translation="MNRIDDHFDEVTALLYLEHQLDPSRAGEVSAHLAACSACSGLLH
                     ALESESLWLRTALAAEEEPVPSYLTAVPQRGATHWGWIAAFFLAMGGLYTLWSGFIQP
                     WYAEASQAGFTQGNLLTMLFFSSAFWKGWDSMRTLLEFLPLATLGAIGIWALRKQWRH
                     FT"
     gene            complement(482..1099)
                     /locus_tag="VN788_09710"
     CDS             complement(482..1099)
                     /locus_tag="VN788_09710"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU20844.1"
                     /translation="MDERKTTGTMSEADLARLLSQVRAGDSDAWGELYRQYAAAIFRF
                     CRRVLPAREDAEDATTEIFMKVRQKLGTYDSSRPFTAWLYKVASNHCWDTLRRRRIRQ
                     DLETGDLDTLPLEHPDPSQIERLQAEHTSKEVRSGLAKLPDRARMALALRYYADMSYD
                     EIADTLGVRRAFVGVLLLRARHQLRDALTQGGAFGALKASAGGKS"
     gene            1360..2193
                     /locus_tag="VN788_09715"
     CDS             1360..2193
                     /locus_tag="VN788_09715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU20845.1"
                     /translation="MWLAAGLMTAGLFLPAVAHGQASPGNSSGPSTNPLSQTDSVRAT
                     EEMNDANMRQHLGDPKEEAAYQAFYKAEDAKKIKLGNAFLSKYPGDRYSEAVYEELAQ
                     TYYDKKDLANFYTYSDKGLSLFPDDVHLLALSGWVIPRAFTPDEPDANKKLDKAEAQA
                     RHALDVMSKMAKPDVFTDEQFTQFKTGESAVAHSGLGLVYFRREKYDESAKELELAIP
                     GEANPDQTDLFILGADYENTSHFKEAADAFNRCAQIAGMMQDRCKQYASVALKNAGEA
                     K"
     gene            2439..2954
                     /locus_tag="VN788_09720"
     CDS             2439..2954
                     /locus_tag="VN788_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU20846.1"
                     /translation="MKKVFTGVVAILFVALLGSQIAAGRNDTEREGQDRAQIEKLMWR
                     YVRALDTENADAYAATYTADGQFGSGASATKGREALKKMILDLRQRNVDAEAKSGKKR
                     PPMYHVLTNSYLEFVDKDHAHMEAYWMTVFAQAGPTVPVRVAAAGREVDELVRVNGQW
                     LIKTRDVAPKD"
     gene            3137..3352
                     /locus_tag="VN788_09725"
     CDS             3137..3352
                     /locus_tag="VN788_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HXU20847.1"
                     /translation="MPIYEYICDECGARYERLVMNGKTVVSCPKCASGKHTLQLSVFA
                     APSNGGKSSAGPSSSGGGCCGGACGCN"
     gene            3428..4564
                     /locus_tag="VN788_09730"
     CDS             3428..4564
                     /locus_tag="VN788_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXU20848.1"
                     /translation="MDPASHDLSRILIELGLIVVSLAILARIASRWGFSAIPLYLLAG
                     LAFGKGGLAPLNVSAGFTHIGAEIGVLLLLFMLGLEYTGAELATNLRSALPAAVVDFA
                     LNFSPGFLAGLLLKWTPLAAILLGGVTYVSSSGVIAKVLAELRRLNNPETPAVLSILV
                     LEDLAMAVYLPLIAVLLAGGGPARMALSVSLALAAVLLVLVLAVRYGVPLSRIAAHQS
                     DEIVLLTIFGAILLVAGAAERLQVSAAIGAFLVGIAVSGPMAEQTHRLLAPLRDLFAA
                     TFFFFFGLQIDPATLPSALPLAILLGAVTATTKVLTGLWAARRTGVDRRGRLRAGMAL
                     IARGEFSIVIAGLGAGLEPQLGALSAAYVLFLAVLGPILARVVK"
     gene            complement(4640..5017)
                     /locus_tag="VN788_09735"
     CDS             complement(4640..5017)
                     /locus_tag="VN788_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018439058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU20849.1"
                     /translation="MSETKSFPYETHLDIAFKALEVIDEKALADACVFKWYNQTLCQV
                     NGSVVRLGVVQGEYHWHKHDADDEFFYVVEGKLLIDLEGRTVELPPRQGFVVPKGILH
                     RTRAPQRTVILMVENAGIVPTGS"
     gene            complement(5120..>5600)
                     /locus_tag="VN788_09740"
     CDS             complement(5120..>5600)
                     /locus_tag="VN788_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU20850.1"
                     /translation="MKLADTANSEKAFQKSIEVSHQKYQDGYLGLAELYLNGHRFAEA
                     ETLARKAVDLDSNSWRANSQLARALVELHRASEAEPSALAAVKLKPDNATLYLLLANI
                     HIQLQNDRAQLDDLNNYLKLAPTGSFAAQARQQRDQLQQFLAASKNSSATSSPAPKP"
BASE COUNT         1175 a         1543 c         1654 g         1228 t
ORIGIN      
        1 gtgaagtgcc tccactgttt tctcagcgcc cagattccaa tcgcccccaa cgtagcgagg
       61 ggtagaaatt ccaatagggt tcgcattgaa tcccatcctt tccaaaaggc gctgctaaag
      121 aaaagcattg tcagcaaatt tccctgcgtg aagccggcct gagaagcctc ggcataccac
      181 ggctgaatga agccgctcca taacgtataa aggccgccca ttgcaagaaa gaaagcggcg
      241 atccatcccc agtgcgtcgc gccgcgctga ggtacggcgg tcagataaga gggaaccggc
      301 tcttcttcgg cggccagcgc tgttcgcagc cagaggcttt cactctcgag agcgtgcagc
      361 aaaccgctgc atgcggaaca cgccgcgaga tgcgccgaga cttcaccggc acggctggga
      421 tcgagctgat gctcgagata gagcagcgcg gtaacttcat cgaagtggtc gtcgattcgg
      481 ttcatgattt tcccccagca gaagctttca gagcgccgaa cgcgccgccc tgcgtgagtg
      541 cgtcgcgcaa ttgatggcgc gcgcgcagca gtaagacgcc cacgaaggcg cggcgcacgc
      601 cgagcgtgtc cgcaatttcg tcgtaactca tgtccgcgta ataacgcagg gccagcgcca
      661 ttcgcgcgcg atcgggcagc ttggccaatc ccgagcggac ttctttgctg gtgtgctcgg
      721 cctgcaagcg ttcgatttgg cttggatcgg gatgctcgag cggcagcgta tccaggtcgc
      781 cggtttcaag gtcctggcga atgcggcggc ggcgcagggt atcccagcaa tgattgctgg
      841 caactttgta gagccacgcg gtaaagggcc gcgacgaatc gtacgttcca agtttttgtc
      901 gcactttcat aaatatttcc gtggtggcat cttccgcatc ctcgcgggcg ggaagcaccc
      961 gcctgcagaa tcgaaaaatc gccgcagcgt attgacgata caattcgccc caggcgtctg
     1021 aatcgcccgc ccggacctgg gacagcaacc gcgccaaatc cgcttcgctc atcgtcccgg
     1081 tcgtcttacg ttcgtccatt gatctaatac gcaggctggc gtcaaatgtt tcacaactat
     1141 cacaaatcag cggagttgtc cgcgagcaat tggattgtgg ccttcaggaa gctcgtcgcc
     1201 gctggtatcg tcgcagaggc cgggcgaatt ccaaaaggac aaaattccct ctcgggtata
     1261 tcatcccgga cacgggcctt ggcgttacgt agctctaagg tcaactcaaa gaagagcaag
     1321 atcaggtgaa ttatgaaatc aagtcgtatg aatcggtgta tgtggttggc cgcggggctg
     1381 atgacggccg gcctgttcct tccggccgtc gcccacggtc aagcaagtcc agggaattca
     1441 tccggccctt cgaccaaccc gctctcgcaa acggactcag ttcgcgcgac cgaggaaatg
     1501 aacgacgcca acatgcgcca gcatttaggc gatccgaaag aggaagcggc gtatcaggca
     1561 ttttacaagg ccgaggacgc gaagaagatc aagcttggca acgcgtttct ctcgaaatat
     1621 cccggggatc gctatagcga agcggtatat gaagagcttg cccaaactta ttatgacaaa
     1681 aaggatctgg cgaactttta cacctattca gacaaggggc tatcgctttt ccccgacgat
     1741 gtgcatttgc tggcgcttag cggctgggtg atcccgcgcg cgtttacccc ggacgagccg
     1801 gatgcgaata aaaaactcga caaagcggaa gcgcaagcga gacatgccct tgacgtgatg
     1861 agcaaaatgg cgaagcccga cgtttttacc gacgaacaat ttactcagtt caagacaggc
     1921 gaatcggcgg tggcccacag cggacttgga ctcgtttact ttcgccgcga gaaatacgac
     1981 gaatccgcga aggaactcga gttggccatc cccggtgaag caaatccgga tcagaccgat
     2041 ctcttcatcc tgggcgccga ctacgaaaat acaagccact tcaaggaagc ggcggacgcg
     2101 ttcaatcgct gcgcgcaaat cgcgggcatg atgcaggatc ggtgcaaaca gtacgcgagc
     2161 gttgctctga aaaacgccgg cgaggcgaag tagaaaatca tcccatcatc atgcaaatag
     2221 gttctgtgac tagcggggtt tgcggccgcg attgtcgcag gatcgtttac tgagccgcaa
     2281 ctaagcgccg gcgtaagttt tgatttcgga tgatgcccgt cggatagccg tgatatagac
     2341 tccctcattc acagaggtgc aaaagctcgg ttcgggagaa tctgttattc ataagcaccc
     2401 tgagaccctg cgcttgttac caaaaggaga tgcgcgtgat gaagaaagtc tttactggcg
     2461 tagttgcgat tttgtttgtg gcattgcttg gctcccagat cgcggcgggg agaaacgata
     2521 cggagcggga aggtcaagac cgcgcgcaga tcgagaagtt gatgtggcgg tatgtccgcg
     2581 ccctggacac ggagaatgcg gacgcttacg ccgccacgta cacggcggac ggacaattcg
     2641 gcagcggcgc cagcgcgacc aaaggacgcg aggcgctcaa gaaaatgata ctggatcttc
     2701 ggcaacgaaa tgttgatgcc gaagcaaaga gtgggaaaaa gaggccgccg atgtaccatg
     2761 tacttaccaa cagctacctc gaatttgtcg ataaggatca cgcgcacatg gaggcttact
     2821 ggatgacggt cttcgctcag gcgggaccca ccgtgcctgt tcgcgtagcg gccgcggggc
     2881 gggaagttga cgaacttgtg cgtgtgaatg ggcagtggct tatcaagacg cgtgacgtgg
     2941 cgccgaagga ctaagtgagg ccggcccggt agttctgttc ggctaaaacc gtgcaggaaa
     3001 atccttaggg agtggtcagc gtgggcaacg acgttcggct tccgatttca aaattcgcca
     3061 gcacgctgga agacgaagcg tcaccgcttt tggtacactg ttgacaccgt aacttcgaga
     3121 tttcgaggcg gacctcatgc caatttacga atatatttgc gacgaatgcg gcgcgcgtta
     3181 cgagcgcctg gtgatgaacg ggaagaccgt ggtttcgtgc ccgaagtgcg cgagcggcaa
     3241 gcacacgctg caattgtcgg tttttgccgc acccagtaac ggcggcaagt cctcggcggg
     3301 gccgagttcc agcggcggcg gttgctgcgg cggcgcctgc ggctgcaact agctcaccta
     3361 aagcatgatg cgcggtacga tgtaaaccaa cagtgcacac tcttagcaga atacagaatc
     3421 gtactgcatg gaccccgcca gtcacgatct atcccgaatc ctaatcgaac tcggactgat
     3481 cgtcgttagt cttgcgattc ttgctcgcat cgcaagtcgc tggggattct ctgccattcc
     3541 tctctatctg ttggccggcc tcgccttcgg caaaggaggg ctggcgccac taaacgtaag
     3601 cgccggcttt acccacatcg gcgccgaaat cggcgtgctt ctgcttctat tcatgctggg
     3661 cctcgaatac accggagccg agttggctac taatcttcgg agcgcacttc ccgcggccgt
     3721 cgtggatttc gcgctgaact tctcgcccgg tttccttgcg gggcttctcc tgaagtggac
     3781 gccgctggcc gcaattctgc taggtggcgt gacctacgtc tcttcgtccg gtgtgattgc
     3841 caaagttttg gcagaacttc ggcggctcaa caatcctgag actccggccg tgctgtcgat
     3901 tttagtgctg gaggacttgg ccatggccgt ctatcttccg ctcatcgccg tgcttctcgc
     3961 cggtggcggc ccggcaagaa tggccctgtc tgtttcattg gcgctggccg cggttcttct
     4021 ggttctcgtg ctggctgtcc gctacggcgt gccgttgagc cgtattgctg ctcatcaatc
     4081 ggatgagatc gtacttctca cgatttttgg cgcgattctt cttgtcgccg gggctgcgga
     4141 acggctgcaa gtatcggccg ctatcggggc atttcttgtg ggcatcgccg tctccggacc
     4201 catggctgaa caaacgcacc gtctgctcgc gcctcttcgc gacttgtttg ccgccacatt
     4261 ctttttcttt tttgggcttc aaatcgaccc ggcaacgctg ccgtccgccc tgcctttagc
     4321 cattttgctg ggcgctgtga ctgcgacgac aaaagtgctg accggcctct gggctgcgcg
     4381 gcgaaccggc gttgatcggc gaggacgctt gcgggcgggc atggccctga tcgcgcgcgg
     4441 cgagttttcc atcgtaattg caggtttggg cgccggcctc gagccacagc tgggagcgct
     4501 ttcggccgcc tatgtgttgt ttctcgccgt gcttggtccc atcctcgccc gcgtggtcaa
     4561 atagtttctc aacgaagtcg gttcttgcca gggataccgc gtgttgcggg aggctggtgt
     4621 ggacggcagc gacgttttat caactgcctg tagggacaat ccccgcattc tcgaccatca
     4681 ggattacagt acgctgcgga gctctggtgc ggtgcaaaat tcctttcgga accacgaagc
     4741 cctgccgcgg aggaagctcg acggttcgac cttccaggtc aatgagaagc ttgccttcca
     4801 cgacatagaa aaattcatcg tcggcgtcat gcttgtgcca atgatattca ccctggacga
     4861 cgccgaggcg caccaccgat ccgttcacct ggcacagtgt ctggttgtac catttgaaaa
     4921 cgcaggcatc cgcgagagct ttctcgtcaa tcacctctag cgccttgaag gcgatatcta
     4981 gatgagtttc gtagggaaat gatttcgtct cgctcatgga atttctcctc gacaccgcgc
     5041 atcggaaatt atcacatcat ttggttggca tcgccgccgg gagacgcgaa tttttggcgt
     5101 gcaaggccac tcaactcacc tacggtttcg gggcggggga tgaggtggcc gaagaatttt
     5161 tcgaagcggc gaggaactgc tgaagttggt cgcgttgttg gcgagcctgc gcggcgaagg
     5221 aaccggtggg ggcgagcttg agatagttgt tcaggtcgtc gagctgggcg cgatcgtttt
     5281 ggagctgtat gtgaatgttg gcaagcagca agtagagcgt ggcattgtcg ggtttgagtt
     5341 tcacagcggc aagggcgctc ggttcggctt cggaggcgcg atgcaattcg acaagcgcac
     5401 gcgcgagttg cgaattagcg cgccaggaat tggaatcaag atcgacagcc ttgcgcgcca
     5461 aagtttcggc ttcagcgaag cggtgcccgt ttaggtaaag ctcagcaagc ccaagatacc
     5521 catcctggta tttctgatgg ctcacttcga tcgacttttg aaacgctttc tcggaattgg
     5581 cggtgtctgc tagtttcatg
//
LOCUS       DATNDT010000301        12631 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1498952, whole genome shotgun sequence.
ACCESSION   DATNDT010000301 DATNDT010000000
VERSION     DATNDT010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12631)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12631)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12631
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1498952"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="VN788_09745"
     CDS             complement(<1..363)
                     /locus_tag="VN788_09745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20851.1"
                     /translation="MALLNSGELQAGERTNADGKAIKNKILLSIPDNEFSAIRPYLQL
                     LDLPHHFSVHEPNQKVEFGYFLNSGMISLVVATEDGKTVEVGVIGNEGFAGSVLAVGL
                     RRSSLREIVQIAGEGLRIE"
     gene            complement(510..857)
                     /locus_tag="VN788_09750"
     CDS             complement(510..857)
                     /locus_tag="VN788_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20852.1"
                     /translation="MKNLSRVSLLALSLLLVAGAIGAGPKLPEHPLKLVLARQSTVPA
                     VDVMKNFSDRCPNISITTNPHRSDFMLYAGGWSGEYRFMVIAKGGDTVFATKTVLLSN
                     AVKDVCRFLNSRP"
     gene            1132..1488
                     /locus_tag="VN788_09755"
     CDS             1132..1488
                     /locus_tag="VN788_09755"
                     /inference="COORDINATES: protein motif:HMM:NF039244.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5818 domain-containing protein"
                     /protein_id="HXU20853.1"
                     /translation="MTVSRKKLMLAPVALLCGAMMFAIGYSFRPSNVSAQQTAPAVVH
                     KHAIARTSMERHTGTILSKNGALLVLADESNNVWYQLDDQEKASSFLGKKVVVFGRLD
                     ASTAVIHVWKIRQIKA"
     gene            1632..2438
                     /locus_tag="VN788_09760"
     CDS             1632..2438
                     /locus_tag="VN788_09760"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXU20854.1"
                     /translation="MASGSTGNATLLETERTTLLIDAGIGRKEILRRFEALGRPCPER
                     LDAILITHEHSDHSSAVAQMARHWDCPAYLTEQTHREIVKMYAGDPEKPGKKAKMEQV
                     EHIRAGERFQIGDIEINPFAIPHDAADPVGYSFRMNGAKVAIVTDLGYMPELVKHHLR
                     EADFLILESNHDLDMLKMGPYPWYIKQRVMSRTGHLSNNVVSEFLSDAELFDGRARHL
                     VLAHLSEQNNNPDLARISAEEALNRRPAEFAFRGSLHIASQRVPLGPFQL"
     gene            complement(2443..2790)
                     /locus_tag="VN788_09765"
     CDS             complement(2443..2790)
                     /locus_tag="VN788_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU20855.1"
                     /translation="MALRMTERDVNGVSFLDVEGRIVLGEESNSFREKVKSLLAAGKK
                     KIVLNLAQVTYIDSAGLGTLVATFHSARSQGATLKLVNLGAKFKEVLQVTKLMTVFDT
                     YENEAAAIQSFAT"
     gene            complement(3186..4445)
                     /gene="rho"
                     /locus_tag="VN788_09770"
     CDS             complement(3186..4445)
                     /gene="rho"
                     /locus_tag="VN788_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HXU20856.1"
                     /translation="MSVSLAELKDKNITDLAKIAKELNIPGSSGMRKQELIFQILRAQ
                     TEKNGLIFSEGVLECLPDGFGFLRAPEYNYLPGPDDIYVSPSQIRKFDLRTGDTVSGQ
                     VRPPKDGERYFALIKVEAVNFEDPEVTRNKIFFDNLTPLYPSERIRLETVKENLTGRV
                     LDMLCPLGKGQRGLIVAPPRTGKTMMLQSIANSITTNHPEIALIVLLIDERPEEVTDM
                     QRTVKGEVISSTFDEPPQRHIQVAEMVLEKAKRLVEHKRDVVILLDSLTRLGRAYNAV
                     TPPSGKVLSGGIDANALQRPRRFFAAARHIEEGGSLTIMATALIDTGSRMDDVIFEEF
                     KGTGNMEINLDRRLVDKRVFPTIDINRSGTRKDELLLPADELNRIWVLRKVLSPLSTV
                     EAMELLLGRLSKTKTNGEFLASMSSPA"
     gene            complement(4626..7034)
                     /gene="lon"
                     /locus_tag="VN788_09775"
     CDS             complement(4626..7034)
                     /gene="lon"
                     /locus_tag="VN788_09775"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXU20857.1"
                     /translation="MFSKEKLDVKRVPMMPVRDMVIFPEMMHPFIVGREASVRALEDA
                     LAGDKRIFLVTQHDASVDDPKPEEIYQVGTLANIVQSVKLPDGNIKVLVEGTERAKIV
                     QVTSDEGFFRASLKLVPPKTEPGPQTQQASERVTGLFEQYVKLSQSLNYDTMIAAVRV
                     DDPSKLSDAIAANLQIPVEEKQELLEIFEPVERLNRIADILDVEIEKLNVDRSINTRV
                     KRQMERAQKEYYLNEKLKAIQKELGRGEANEIEQLKKKIETSGMPEGPMEKAVQELKR
                     LEMMPPMSAESTVSRNYLDWLLAVPWKKRSKEIRDIKRAETILSEDHYGLEKIKERIL
                     EYLAVRQLVKNPKGSILCFLGPPGVGKTSLAMSIGRATGRKFVRVSLGGVRDEAEIRG
                     HRRTYIGALPGQIIQMMRKAGTTNPVFVLDEVDKMSMDFRGDPSAALMEVLDPELNHS
                     FTDHYLDVEYDLSKVMFICTANVLHTIPQPLQDRMEVLRLPGYTEQEKHQIAQRFLVG
                     RQLEATGLSKENLKFTDEAITHIIRHYTHEAGVRNLDREIANVCRKMARKVVAEGKKV
                     AEEVTPANAKDYLGILKYRDFLAEKKNEIGLTTGLAWTEVGGEVLSTEATLMQGKGRL
                     TLTGKLGDVMQESAQAGMSYVRSRSHLFGLPKDFYRHLDIHVHVPEGAIPKDGPSAGI
                     TLCTSIVSALTRIPVRCDLAMTGEITLRGKVLPIGGVKEKLLAAHRLGLHTVILPKDN
                     EKDLADIPPEIQAQMSIHFVESMDEVLQLALETTLPISAQPPVDGVEPKFGVDVTQDN
                     ELTN"
     gene            complement(7095..8345)
                     /gene="clpX"
                     /locus_tag="VN788_09780"
     CDS             complement(7095..8345)
                     /gene="clpX"
                     /locus_tag="VN788_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXU20858.1"
                     /translation="MKRFTPEEPLRCSFCHKTQEQVEKLISSPSEYPRSYICNECVGV
                     CQQILEDEKREQSSPANRRLPRPPEIKAFLDSYVIGQDKTKKKLAVAVYNHYKRIFLN
                     KQPGDVELTKSNILLIGPTGTGKTLLAQTLSRMLEVPFAIVDATTLTEAGYVGEDVEN
                     IILKLLQAADGDVQKAQQGIIYIDEIDKISKKDENPSITRDVSGEGVQQALLKILEGT
                     VANVPPQGGRKHPHQEFTPVDTTNILFICGGAFVGLEKTIERRVGQKSLGFHADAQPS
                     TATRRNIEVLEQAQPTDLIRYGLIPEFVGRLPVMGVLSDLDKSALVRILTEPKNALVR
                     QYQRLFEFENVRLRFTDDALETVADLALQRKVGARGLRMILEDLMLEMMYHLPAQRKL
                     RDFVVTADMVKGREINWSLLEKAG"
     gene            complement(8463..9083)
                     /gene="clpP"
                     /locus_tag="VN788_09785"
     CDS             complement(8463..9083)
                     /gene="clpP"
                     /locus_tag="VN788_09785"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HXU20859.1"
                     /translation="MRQTDDSSPRATTLVPMVVEQTNRGERAYDIYSRLLKDHIIFIG
                     TPIDDHVANLVTAQLLFLESEDPEKDISVYINSPGGSISAGMAIYDTMQFVRPDVVTY
                     CIGQSASIAAVLLAAGTPKKRYALPNSRVLIHQPWMSGLSGQATDIDIHAKEIIRTRA
                     ALNKLLANHTGQSIEKIEKDVERDFIMTPEQAKAYGIVDEIITKHR"
     gene            complement(9123..10424)
                     /gene="tig"
                     /locus_tag="VN788_09790"
     CDS             complement(9123..10424)
                     /gene="tig"
                     /locus_tag="VN788_09790"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXU20860.1"
                     /translation="MTEATCKREVELEIPAENVEKATEKVARDIARIARIPGFRPGKA
                     PITLVRRRFAGDIQGEVVQSLVPEYLEKALDEKKLVPVARPEVDKVEFKEGEPLKFRA
                     IFEVLPEFELGDYKNLQVQVDEIEVGDAQVDKTLEEMRERAATFVPVEGRAAKDGDSV
                     LIKLIGTPSGGGEPVQAENVMVPLGAEETLPVFSENLRGATTGETKRFDARYPDDYPD
                     PKLAGKTYDYTVEVQVIKEKKLPELNDEFVKDAVGAEGSQLSGVATLDELRKKIREGL
                     DAEKEHREKTQGREKVLETLVNQHDFPVPEALIEGQMDSRLERVVRSLAAQGVDPRGM
                     NVDWTALRRQQRERAIADVKAELILDRIATAENIDVTDEEVEKEIATLAERSGESATA
                     LRARLTKQGAVDRMKSKLRSDKTVDSLYRTARIETISRGAK"
     gene            complement(10645..10731)
                     /locus_tag="VN788_09795"
     tRNA            complement(10645..10731)
                     /locus_tag="VN788_09795"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10695..10697),aa:Leu,seq:tag)
     gene            10979..11323
                     /locus_tag="VN788_09800"
     CDS             10979..11323
                     /locus_tag="VN788_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010870963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HXU20861.1"
                     /translation="MSENVAGLLCYVLGWITGLIFYFVDKRPFVRFHAAQSIVVFGGL
                     HILYFIVAVFFGMSLFTGGLGGFSLVWMLYGLVDLAAFILWIVLMIKAYQGTRFRVPI
                     AADIADKIFPHT"
     gene            11541..>12631
                     /locus_tag="VN788_09805"
     CDS             11541..>12631
                     /locus_tag="VN788_09805"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HXU20862.1"
                     /translation="MAEKVRTKAIAVVGAQWGDEGKGKVVDYLASSFDYTARYAGGHN
                     AGHTVIFNGHRYVLQLVPSGVLRPEKKAVIGPGAVVDPAALVAELENLRKSGVEVRGR
                     LFLSNRAHLIFPYHREMDKAAESARGEAKIGTTSRGIGPAYEDKMARRGIRVCELLEP
                     DCFRIKLERVMAEKNAISQAAYGHSLETAAVLEQYLELAAHIRGLAADTAVLLNGALD
                     RGESVLFEGAQGTMLDIDHGTYPYVTSSNAISGGACAGLGVAPTRITGVVGVTKAYTT
                     RVGSGPFPTEMPDLDAQEVRARGNEFGAVTGRPRRCGWLDLVVLRYAKMLNGIDSLVV
                     TKLDVFDEQPEIKVCTGYRHKGQPLAEMPP"
BASE COUNT         2496 a         3711 c         3423 g         3001 t
ORIGIN      
        1 ttctatccga agaccctcac cggctatctg cacgatttcg cgaagtgagc ttcttctcag
       61 gccaacagcg agaacggatc ccgcgaagcc ctcgtttcca atcacgccga cttcgactgt
      121 tttaccgtct tctgtggcaa ccacgagcga gatcattccg ctattcagga aataaccgaa
      181 ctcaactttt tgatttggtt catgcacgct aaagtggtgc ggaaggtcta ggagttgcag
      241 gtaagggcgg attgcactaa attcgttatc tggaatcgat aacagaatct tgtttttgat
      301 cgccttgccg tcagcgttag tgcgctcacc ggcctgcaat tccccagagt tcaacagagc
      361 catagttaac tcgtctattc gcgagtagtt gaggataaag caatagtaac aggacaaagc
      421 tgcgcgtaca attttcgtaa gggcggaggc cgattagcgg tgggtgaacg atgtctactg
      481 tgggcatccg gcggaggggg ccagccggac tagggcctcg agttcaagaa tctgcagaca
      541 tccttaacgg cgttgctcaa gagtacggtt ttcgtggcaa aaaccgtgtc gccgccttta
      601 gcgataacca tgaaacgata ttcgcccgac cagcctcctg cgtacagcat gaaatcggat
      661 cggtgcggat tggttgtaat gctgatgttc gggcaccgat cactgaaatt cttcatcacg
      721 tccaccgccg gcacggtgga ttgacgcgcc aggaccagtt tcaatggatg ctccggcagc
      781 ttgggcccgg ctccaattgc accggccact agcaacagag atagggctag caacgatacc
      841 cgtgagaggt ttttcatgac ggctccttca ccgtttgtct cataccttag aacccgccgg
      901 accgaaaaat gttgtgtcga tgccccactc atcaagttgg cggatcgatg taagcactta
      961 cttttcaaac ggaagcacca ttaggacttt ttttctggct gtcgagcttt ctacatcccc
     1021 tgtggtgtca cgcgagcgcc tcgctacaac agccgactgg ttttgctggc attcgtaaat
     1081 ggtacataac ttgctctagc taaggcagcc tggtttttgt ggagaaccaa aatgacggtc
     1141 agtcgcaaga aactaatgct cgcaccggtg gcgcttctgt gcggcgccat gatgttcgcg
     1201 atcggctata gctttcggcc gtcgaatgtt tccgctcagc aaacagcgcc agcggtcgtc
     1261 cacaagcacg ccatagctag aacctctatg gagcgacata ccgggacgat cttgtcgaag
     1321 aacggcgcgc tgctggttct cgccgatgaa agcaataatg tgtggtatca gctcgacgat
     1381 caggaaaaag ccagcagctt tctcggcaaa aaggttgtgg tgtttggcag gttggacgcc
     1441 tcgacagccg tgatccacgt ctggaagatc cggcagatca aagcgtaact gaggcaacgc
     1501 ccggctcgcc gccatttttt gcatcgctaa cctcatctcc ttcttgacgc tgaccctccg
     1561 ctctgccaat atcctgccgt ctccaaattt agcgggctga gggcgatcgc atctcgttcc
     1621 gcatttcaat tctggcctct ggtagcacgg gcaacgcaac gttgctcgaa accgaacgca
     1681 ccaccctcct catcgatgcg ggaataggac gcaaggaaat tctacggcgg tttgaagcgt
     1741 tgggtcggcc ctgcccggaa cgcttggacg ccatcctaat cactcacgag cattccgacc
     1801 attccagcgc cgtcgcgcaa atggcgcgcc actgggattg tcctgcgtat ctcaccgagc
     1861 agacgcatcg cgaaattgtg aaaatgtacg cgggcgatcc cgaaaagccc ggtaagaaag
     1921 caaagatgga gcaggtggaa cacattcgcg ctggagagcg cttccagatc ggcgacatcg
     1981 aaatcaatcc attcgctatc ccgcacgacg cagcggatcc ggtcggctat tccttccgaa
     2041 tgaatggagc aaaagtcgcg atcgtcacgg acctcggcta catgcccgaa cttgtgaagc
     2101 atcatctgcg cgaggctgac ttcctgattc tcgaatccaa tcacgatctc gacatgctga
     2161 agatgggccc ctatccgtgg tatatcaagc agcgcgtgat gagccgcacg gggcatctct
     2221 cgaataatgt cgtcagcgaa tttctttcgg atgccgagct attcgacggc cgcgcccgcc
     2281 atctcgtcct cgcgcactta tcggagcaaa ataataatcc ggatctggcg cgcatctccg
     2341 ccgaggaagc gctgaaccgc cgcccggctg agttcgcctt ccgtgggtcg ctgcatatcg
     2401 cctcgcagcg cgtcccgctc ggccccttcc aactgtagtc tattaagtag cgaagctctg
     2461 aatggcggcg gcctcattct catacgtgtc gaagaccgtc atcagcttcg tcacctgcag
     2521 aacttccttg aatttcgcgc ccaggttcac cagcttcaac gtggccccct gcgaccgcgc
     2581 gctatggaat gtagccacca gagtgcccag gccggcgctg tctatgtagg tcacctgcgc
     2641 gagatttaac acgatcttct tcttgccggc cgccagcaag ctcttcacct tctcgcggaa
     2701 agagttgctc tcttcgccca gcacgatgcg cccttcaacg tccaaaaagc tcactccgtt
     2761 cacatcccgt tcggtcattc gcagtgccac gttagttcct cctgaacttg aaacatcgct
     2821 tctatggggt tgcaaaaatt gaaaagcaca acggctccgg attgtcctcg cgctctcctc
     2881 agtaaaaatc cgagcaggac atataatacc cgcaattagt gcaaatcagt ttgcaaccgc
     2941 gcgatgccaa gcgggccgag cagcgcggac agtagttcaa cgccagttcc gtagcggtat
     3001 gagattcgct tggcgctgta ctcaattctc cgagctcgcc aatcgaatcg cccgacttat
     3061 cggcattcct caggggttcc acaccgcccc tccgagcggg tatttatagc acatcccatc
     3121 cattgcaaga agaaaagggt ctggtatttg acggtattcg gcgcaaggtg ggatttcgac
     3181 agccctcagg ctgggctgga catggaagcc aaaaactccc cgttcgtctt ggtctttgac
     3241 aggcggccga gcagcagctc catcgcttcg acggtcgata gcgggctgag aaccttgcgc
     3301 agcacccaga tgcggttcag ttcgtcggcc ggcaagagca attcatcctt gcgcgttccc
     3361 gagcggttga tgtcgatcgt cggaaacact cgcttgtcca ccaacctgcg gtccagattg
     3421 atttccatgt tcccggtgcc cttgaactct tcgaaaatca cgtcgtccat ccggctgccc
     3481 gtatcgatca gcgccgtagc catgatcgtc aacgacccgc cctcttcgat gtgtcgcgcc
     3541 gcagcgaaga atctgcgagg acgctgcaag gcattcgcgt cgataccgcc cgaaagaacc
     3601 ttgcccgaag gcggagtgac agcattgtag gcgcggccca ggcgcgtcag cgagtcgagc
     3661 aggatcacca cgtcgcgctt gtgctccacc agtcgcttgg ccttttccag caccatttcc
     3721 gctacttgaa tgtggcgttg cggcggctca tcgaacgtcg agctgatcac ttcgcctttc
     3781 accgtccgct gcatgtccgt aacttcttcc ggccgctcgt cgatcagcag gacgatcagc
     3841 gcgatttccg gatgattcgt ggtgatcgaa ttggcgatgg attgcaacat catggttttg
     3901 ccggtgcgcg gcggcgccac gatcaatccc cgctgtcctt tgcctagagg gcaaagcatg
     3961 tccagcacgc gtcccgtcag attttctttc acagtttcca ggcgaatccg ttcggaaggg
     4021 taaagcggtg tgagattgtc gaaaaagatc ttgttgcgtg tgacctcagg atcctcgaaa
     4081 ttcaccgcct ccactttgat cagcgcgaag tagcgctcgc cgtctttcgg cggccgaacc
     4141 tggccggaga cggtatcgcc tgtgcgcaag tcgaatttcc gtatctgcga tggcgacacg
     4201 tagatatcgt ccggcccggg caaatagttg tactcgggcg cgcgaaggaa gccgaagcca
     4261 tccggcaggc attccagaac cccttcggag aaaatcaggc cgttcttttc ggtttgcgcg
     4321 cggaggattt ggaagatcag ttcctgcttg cgcattcccg aggagccggg aatgttcaat
     4381 tccttggcga tcttggccaa atctgtaatg tttttgtctt ttagttctgc aaggctaaca
     4441 ctcatcgatt tctttccttc tcaagcctaa atactctcag gcacgattat tgggtgaaat
     4501 aaatccgggc gggcgccttc gtttgatgca ctctatccca gattccaaaa cgggtacgta
     4561 cggaaaacct tacactccgg tcgcggaccg aacctgccga gcaggatgtg ggaggaccga
     4621 aggactcaat tggtcagttc attgtcctgc gtgacgtcca cgccgaactt gggttccact
     4681 ccgtcaaccg gcggctgtgc cgaaatcggc agcgttgttt caagcgcaag ctgcagcact
     4741 tcatccatgg actcgacgaa atggatcgac atctgcgctt gaatctccgg cggaatatcg
     4801 gccagatcct tctcgttatc tttcggcagg atcacggtgt gcaggccgag ccggtgcgcc
     4861 gcgagcaatt tctcttttac cccgccgata ggaagaacct tgccgcgcag agtgatttcg
     4921 ccggtcatgg caaggtcaca gcgaaccgga atccgcgtaa gcgcgctcac gatggaggtg
     4981 cagagagtga ttccagcgga agggccgtcc ttgggaatgg cgccttccgg gacgtggacg
     5041 tggatatcca gatggcggta aaaatccttg ggcaggccaa aaagatggct gcgcgagcgg
     5101 acgtagctca ttcccgcttg tgcggattcc tgcatcacgt cgcccagctt gccggttaac
     5161 gtcagccggc ccttgccttg catcagcgtg gcttcggttg aaagcacttc cccgccaact
     5221 tccgtccagg cgagtccggt cgtcaatcca atctcgtttt tcttctcagc caggaagtcg
     5281 cggtatttca atatgcccag ataatccttg gcgttcgccg gcgttacttc ctccgccacc
     5341 tttttccctt cggcgacaac tttacgggcc atcttgcggc acacattcgc gatctcgcga
     5401 tccaggttgc gcacgcccgc ttcatgggtg tagtggcgga tgatgtgcgt gatcgcttcg
     5461 tcggtgaatt tcaggttctc cttgcttaag ccggtggctt ccagttgccg cccgacaaga
     5521 aaacgctgcg cgatctgatg cttttcctgc tccgtgtagc ccgggagccg caaaacttcc
     5581 atgcgatcct gcagcggctg cggaatcgtg tgcagcacgt tcgcggtgca gatgaacatc
     5641 accttcgaaa gatcgtattc cacgtccaaa taatggtcgg tgaaggaatg attcagctcc
     5701 ggatcgagca cttccatcag cgcagcggaa ggatcgccgc gaaaatccat ggacatttta
     5761 tcgacttcat ccaggacgaa aaccgggttc gtggtgcccg ccttgcgcat catctggatg
     5821 atttgccccg gaagggcccc gatatacgtt cgacgatgtc cgcggatttc agcctcgtcg
     5881 cgcacgccgc ccagagaaac gcggacaaat tttctgcccg tggcgcggcc gatcgacatc
     5941 gccagcgaag tcttgccaac tcctggaggg ccaaggaaac acaggatcga gccttttgga
     6001 ttttttacca gttggcgcac cgccaggtat tcgaggatgc gctctttgat tttttccagg
     6061 ccgtagtgat cctcggaaag aattgtttcg gcacgcttga tatcgcgaat ttccttggag
     6121 cgctttttcc acggcaccgc aagcagccag tccagataat tgcgcgacac ggtggattcg
     6181 gcggacatgg gcggcatcat ttccagccgc ttcagctcct gtacggcctt ttccatcggc
     6241 ccttcaggca tgccggaagt ctcgattttc ttcttcagtt gctcgatttc gttcgcttca
     6301 ccgcgcccta gctccttctg aatcgctttc agtttttcgt tgaggtaata ctctttttgg
     6361 gcgcgctcca tctggcgttt cacgcgagtg ttaatcgagc gatcgacgtt gagcttttcg
     6421 atttctacat ccagaatatc ggcgatccgg ttcaaccgct ccaccggctc gaatatctcc
     6481 agcagttcct gtttttcctc caccggaatt tggagatttg ctgcgatggc atcggaaagt
     6541 ttgctcggat catccacgcg caccgcggcg atcattgtgt cgtaattcaa gctctgcgag
     6601 agcttcacgt attgctcaaa aagtcccgtc acgcgctcgg aggcctgctg cgtttgcggt
     6661 cccggctcgg tcttcggcgg gacgagcttg agcgaagccc taaaaaatcc ttcgtcgctg
     6721 gttacctgca cgattttcgc gcgctccgtg ccttcgacca gcacctttat attgccgtct
     6781 ggtagcttca cgctctgcac gatgttggcg agcgtgccta cctggtagat ttcttccggc
     6841 ttcggatcgt ccacggaggc gtcgtgctgt gtgaccagaa atattctctt gtcgccggcc
     6901 aacgcgtctt ccagcgcgcg caccgaggcc tcgcggccca cgatgaaggg gtgcatcatt
     6961 tccgggaaaa tgaccatgtc ccgcaccggc atcatcggca cgcgcttcac gtcgagtttt
     7021 tccttgctga acataacctt gaaatcgcgg cccctatgcc gcgaaagcct tgctttcgca
     7081 aatgaatctg aaaattaacc cgctttttcg aggaggctcc aattgatctc gcggcccttc
     7141 accatatccg ccgtcaccac gaaatcgcgg agtttccgct gggccggcag gtgatacatc
     7201 atttccagca tcaagtcttc gaggatcatc cggaggccgc gcgcgcccac tttgcgctga
     7261 agggccaggt ctgcaacggt ttccagtgcg tcgtcggtga agcgcaggcg gacgttttcg
     7321 aactcaaaga gtctttgata ttgacgcacc agcgcgtttt tcggttccgt cagaatccgt
     7381 accagagcgc tcttgtcaag atcgctcagc actcccatca ccggcagcct gcccacgaat
     7441 tctggaatca ggccataccg gatcaggtcc gttggctgcg cctgctcgag cacctcaatg
     7501 ttgcgccgcg tggccgtcga tggctgcgcg tccgcatgga aacccaggga tttttggcct
     7561 acccggcgtt cgatcgtctt ttccaatccc acgaaggcgc cgccgcaaat gaataagata
     7621 ttcgtcgtat cgactggtgt gaactcctgg tgcgggtgct tgcgtccacc ttgcggcggc
     7681 acgttggcca cggtgccttc aagaatcttc aatagcgcct gctgcactcc ttcgcccgag
     7741 acatcccgcg tgatcgatgg gttctcatcc ttcttcgaaa tcttgtcgat ctcgtctata
     7801 tatatgatgc cttgctgggc cttttggacg tcgccatcgg cagcctgaag cagctttaga
     7861 ataatatttt cgacgtcctc tcccacatag cccgcttctg tcagcgtcgt cgcatccacg
     7921 atcgcgaacg gcacctcgag catccgcgag agtgtctgcg ccaggagcgt tttgcccgtc
     7981 ccggttggcc cgatcagcag gatgttggac ttggtcaact ccacgtcgcc cggttgctta
     8041 ttcaggaaaa tgcgcttgta gtggttgtag actgccacag cgagcttctt ctttgttttg
     8101 tcctgtccaa tcacgtaact gtcgagaaaa gccttgatct ctggcgggcg cggcaggcgg
     8161 cggtttgccg ggctggattg ttcgcgcttc tcgtcctcga gaatctgctg gcagacgccc
     8221 acacattcat tgcagatata ggagcgcggg tattccgatg gggagctgat gagcttttcc
     8281 acctgctcct gcgttttatg gcagaaggaa cagcgcagcg gctcttccgg ggtgaaccgt
     8341 ttcacttgtt acgccccctc tggcaaaccc tatggatggt gaaccctgaa accattgtag
     8401 aacggaatac tcgccttcgt cccgcgcatt caccgcacca ctgctaacta tttggaacgc
     8461 ctttaccggt gcttggtgat gatctcatcc acgatgccgt aggctttggc ctgctccggc
     8521 gtcatgatga agtcgcgttc cacgtctttt tcaatttttt cgatcgattg tcccgtgtga
     8581 tttgccagca gcttgttcag cgccgcacga gttcggataa tttccttcgc gtggatatcg
     8641 atgtcggtag cctggcctga aaggccggac atccatggtt ggtgaatgag tacgcgggaa
     8701 ttgggcagtg cataacgctt tttaggggtg cccgccgcaa gcaacaccgc tgcaatcgag
     8761 gccgattggc cgatgcagta ggtgactaca tccggccgga cgaattgcat cgtgtcgtaa
     8821 atagccatac ccgccgaaat cgatcccccc ggagagttga tgtaaaccga aatatccttt
     8881 tcgggatctt cggattcgag gaagagcagt tgggccgtga cgagattggc tacgtgatcg
     8941 tcaattggag tgccaataaa gatgatgtgg tccttgagaa ggcgggaata gatatcgtaa
     9001 gcgcgctcgc cacgattggt ttgctccaca accatgggaa ctagcgtcgt tgcgcgcgga
     9061 ctcgaatcgt cagtttgtct catttggaat gatcgtgcgc actgagggag gcccgcccca
     9121 aattactttg cacccctgga tatcgtctcg attcgtgccg tgcggtatag cgaatcgact
     9181 gttttgtcgc ttcgcaactt cgatttcatc ctatccaccg ctccttgctt tgtcaagcga
     9241 gcgcgaagcg cggttgcgga ttcaccactg cgttctgcca gggttgctat ctctttttct
     9301 acttcttcgt cagtgacatc gatattttct gcggtggcaa tgcggtcgag gatcagttcc
     9361 gcctttacat cggcaattgc gcgctcgcgc tgctgccttc gcaacgcggt ccaatccacg
     9421 ttcatccccc gcggatccac tccctgcgcc gccagcgacc gcaccacgcg ctccagccgg
     9481 ctatccatct ggccttcgat cagcgcttcg gggacgggaa agtcatgctg attcacaagt
     9541 gtctcgagaa ctttttcgcg gccctgagtt ttttcccggt gctctttttc ggcgtccagg
     9601 ccctcgcgaa ttttctttcg gagttcatcg agcgtcgcaa cgccgcttaa ctgcgagcct
     9661 tcggcgccga ccgcatcctt tacgaattcg tcgttgagtt cgggcagctt cttttccttg
     9721 attacctgca cctcgaccgt gtagtcgtat gtttttcccg ccagcttcgg atcgggataa
     9781 tcgtcgggat agcgcgcatc gaaacgcttc gtctctcctg tagtggcccc gcgcaaattc
     9841 tcgctaaaaa cgggcaacgt ttcttccgcg cccaatggaa ccatcacatt ctcggcctgc
     9901 accggttcgc ccccgcccga tggcgtgcca atcaacttga tgagaaccga atccccgtct
     9961 tttgccgcgc gaccttctac cggaacaaac gtcgcggcgc gctcgcgcat ctcttccagc
    10021 gttttatcca cttgcgcgtc gcccacttcg atttcgtcaa cctgcacctg aagatttttg
    10081 taatcgccca actcgaattc cggaagcacc tcaaaaatcg cccggaattt cagcggctcg
    10141 ccttccttga actcaacttt gtccacttcc ggccgcgcca ccggcaccag cttcttttca
    10201 tccagcgctt tttccaaata ttccggtacc agcgactgca caacttctcc ctgaatatcg
    10261 ccggcgaatc tgcgccgcac cagagtgatc ggcgccttgc cgggacggaa tccggggatg
    10321 cgggcgattc gcgcgatgtc gcgcgccacc ttttccgtcg ctttctcgac gttttcagcc
    10381 ggtatttcca gctcaacttc gcgcttgcag gttgcttcag tcatggttcg tgagagattg
    10441 ccctggatgt taacgccagt gccccagttt caatcctctt gcctgttggg aatcggtagg
    10501 aggaatgcag ggaagagcgg cgacactatc gcaggtgaaa ttcgagtata gggaatctac
    10561 ccgcaggcgt caactgcgga cgcctcgcta ccgcggttcc ccggtgagtt cagttggcgc
    10621 ggtcaacgca atcgcaatag ttgctggtgc ggaagaaggg actcgaaccc ctacggcctt
    10681 tcggccacca gctcctaagg ctggcgcgtc tgccaattcc gccacttccg cgcagaaatg
    10741 aagtatatat catcgcaacc aaggcagtcc tgcgattacg cttgctcgca ctggcgactt
    10801 ttgataggct gcgccgcaat acaagccttc cagcggaggg aacaccatgg cacattgcac
    10861 aaaatgcggg gcagccgtcc tggacaattc ttccttttgc gccgcatgcg gagcgcctca
    10921 gggcgtagcc gtcgcggctc caggcgcgcc ggccgttgtc tcggggagtc aatcgcaaat
    10981 gtctgaaaat gtcgccggcc tgctctgcta cgttctcggc tggataacgg ggctgatttt
    11041 ctatttcgtc gataagcggc ccttcgtgcg tttccacgcc gcgcagtcca tcgtggtctt
    11101 cggcggtctt cacatccttt attttattgt ggccgtcttt ttcgggatga gtttgtttac
    11161 cggcggcctc ggtggttttt cgctggtatg gatgttgtac ggccttgtcg atctggccgc
    11221 ttttattctc tggatcgtgc tgatgatcaa ggcgtaccag ggaacgcggt ttcgcgtgcc
    11281 gatcgcggcc gacatcgcag acaaaatctt tccgcatacc taatcggttt gcagcctgcg
    11341 ggctgagcgg aggtcccgct gctccgcgcg gcggctttgc tgccacactg tttagcaacc
    11401 agggcagtcc cgctattgcc ttcgcctgac gcgcggcgta cgattgtcgc ttggtatttg
    11461 tcgcactagc cttcctgtaa gctttcattt gagacgcagc gggcgagttg cggttcgtgt
    11521 gtggttctgg agcgttccga gtggcggaga aagtccgaac gaaggcgatc gccgtggtgg
    11581 gcgcccaatg gggcgacgaa ggcaagggca aagttgtcga ttacctggca tcatcttttg
    11641 attacacggc gcgctacgcg ggcggccaca atgcgggtca caccgtcatt tttaatggcc
    11701 atcgctacgt gctgcaactg gttcccagcg gcgttctgcg gccggaaaaa aaagccgtca
    11761 tcgggcccgg agctgtggtc gatcccgcgg cgcttgttgc cgagcttgaa aacctgcgca
    11821 agtcaggcgt ggaagtgcgc gggcgccttt tcctcagcaa tcgcgcgcat ctgatttttc
    11881 cgtatcaccg cgaaatggac aaagcggcgg aatccgcgcg tggcgaagcg aaaatcggaa
    11941 ccacgtcgcg cggcatcggt ccggcatacg aggacaaaat ggcgcgccgc ggaatccgtg
    12001 tgtgcgaact gctcgagccg gattgcttcc gcatcaagct cgagcgcgtg atggcagaaa
    12061 aaaatgccat cagccaagcc gcttacggac attccctcga gactgccgcc gtgctcgaac
    12121 agtatctcga actcgcggcg cacattcgcg ggctcgctgc cgacactgcc gtactgctga
    12181 atggcgcgct cgatcgcggc gaatccgtgc tctttgaggg cgctcaaggc acgatgctgg
    12241 acatcgatca tggaacctat ccttacgtaa cctcgtcgaa cgccatctcg ggcggtgcat
    12301 gcgcgggtct gggcgttgcg cctacccgga tcaccggagt agtcggcgtg acgaaggcgt
    12361 acaccacgcg cgtgggcagc ggtccatttc ctaccgagat gcccgatctc gatgctcagg
    12421 aagttcgcgc gcgcggcaac gaattcggcg cagtcacggg acgtccacgg cgttgcggct
    12481 ggctcgattt agtggttttg cgctacgcga aaatgctcaa tggaatcgat tcgctggttg
    12541 tgacgaagct cgatgtattc gacgagcagc ccgagatcaa agtttgcaca ggctaccgtc
    12601 acaaaggcca accgctcgcg gaaatgccgc c
//
LOCUS       DATNDT010000302         6629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2651866, whole genome shotgun sequence.
ACCESSION   DATNDT010000302 DATNDT010000000
VERSION     DATNDT010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6629
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2651866"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(981..2222)
                     /locus_tag="VN788_09810"
     CDS             complement(981..2222)
                     /locus_tag="VN788_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20863.1"
                     /translation="MKQDIRENLVLAFDTLRTHKTRSLLAVLGVVIGVGVIIIVASLI
                     TGFKASINEIIEGFGADTAYVNRFDNGPGAGRSLKQARERPPLTLEQGQAILASCPDV
                     KQIAITIFNRFQIHLIKYGRNQAQNIDFRGTFPAYMEVYGNAQLLAGRFFNEAENEHR
                     SKVVVLGEDTAKALFPTPQDALQKTIAIDGSDFLVIGVFSKPPGGFGLNDQDIRVMIP
                     YYTYQKMFPSVQDNGYRFLAVHGKVDEAVDEATEVLRRERKVPYRAPDNFSITTQVQQ
                     EEQFNQIIGGVALVLVVLSSIGLLIGGVGVMNIMLVSVTERTREIGVRKAIGARSRDI
                     TWQFLFEAMTLTGAGGILGVAFFSALAMLIPHITSMRASVPLWAVIVGIVVSVSIGLV
                     FGVWPAVKAAKLDPIEALRYE"
     gene            complement(2232..3500)
                     /locus_tag="VN788_09815"
     CDS             complement(2232..3500)
                     /locus_tag="VN788_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20864.1"
                     /translation="MQKDPKLKLGFRQTMIALREATALAFDTLRAHKLRSFLTLLGVI
                     LAVTTLVSVMSVLNGLNLYVATKIANLGANAFVIDRVGIITNFDDFVKAMRRPPLHMD
                     DYYALKGHLQYSSEVAAEENTVQDVHYGEKLIEDVTVIGATPEFAVVRDFDVVAGRFF
                     TDVDETHRTPTCFIGHDVQVNLFPGVDPLGREIRVGAQQCQVIGVAKALGTLLGQTQD
                     NFVMLPLTTYMSVFQGPNDSVTIFVEAVTTEAMPIAEDEVRVMLRARRHVRYQDPDNF
                     GIIMPSSITGLWQSLTGNIFGIAIWLTSVFLVVGGIVIMNIMLASVTERTREIGLRKS
                     LGARRKHIVLQFMMESSILAATGGAMGILAAFGIAMLVRAASPMPISTPLHAVIIALA
                     LSTGVGLFFGIYPAIRASRLDPIEALRAEA"
     gene            3680..4654
                     /locus_tag="VN788_09820"
     CDS             3680..4654
                     /locus_tag="VN788_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20865.1"
                     /translation="MKGRSPIRLAAIALAGILCGIQPCATAQMQGQPQSPPLDQTPLT
                     AKQLDDLISRTIANQHRNDAAADSFERIEHHVSHSGSADGPVTQDKTYRVVPTGSGTL
                     KLLIRDSSKNVSDAFYRHQLRDWENILKVAVHPDDPRQVAVMAKQQKRQKDRARFIDA
                     ANGAYKIRWLAREIRDGRIVEKLQLDPNPAYKPRGDATDWLMHAQAIIWIDPQEAQAA
                     AIDATITRDISIGGGFLGKIYRGGHFLMQQAPVASGIWEPTHYEYDISGRKFLFGFTM
                     HEEISDSHYRLLGPPQQLLAEAQNSLAACCDIDALTMGATLHSGGGPH"
     gene            complement(4623..5309)
                     /locus_tag="VN788_09825"
     CDS             complement(4623..5309)
                     /locus_tag="VN788_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20866.1"
                     /translation="MRTYLIGPFLAFLPPAWRDAYFCDWKMDWPRAAAISGLLEGFAA
                     FVALVVWYSMYVTRIGEIIGAGGQFGGYVGLFSTAIHPLTWLICYFGCEGTVRLLAAV
                     VNGESYGTLPLWLLSWRSNKAGVRGKAVADRVIMGGTCCDLEVSSFRPKENWKYPLTI
                     RYQDEFFQVMGEERKAPSEPWPHVYFLRRLPANEIIKGMETYDPNAIPEAPPGFFETV
                     FSEVRRRYAK"
     gene            5481..5789
                     /locus_tag="VN788_09830"
     CDS             5481..5789
                     /locus_tag="VN788_09830"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HXU20867.1"
                     /translation="MYARVTQFHLGPDKLEEFVDAVRAAIPHAHQQKGFRALLVLRDD
                     AGATSAHVRVISVWNSAEDLQAAEQNFYLYQALAKVMTATKGFPLIEGQEVLLAELDS
                     "
     gene            5965..>6629
                     /locus_tag="VN788_09835"
     CDS             5965..>6629
                     /locus_tag="VN788_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HXU20868.1"
                     /translation="MSTIQITLPDGAKREVNSGTTPAQIARQISPRLEKEALVARVDG
                     QLWDLSRPLDESTSLAILTPKDPEAIQVLRHSAAHLMAAAVIELFPNVKLGIGPPIDN
                     GFFYEFLREEPFTPEDLERIEKKMHELASKDIPNERKLLPKPEALKLYRESNQEFKCE
                     LVEEKAIEPMVSFYITGKFIDFCRGPHIPSTGRIRAFKLTNVAGAYWKGQESNPQMQR
                     IYAL"
BASE COUNT         1549 a         1831 c         1939 g         1310 t
ORIGIN      
        1 gcacggtggc gttggggacg gcggcgttgc tggggtcggt gacgacgccg cgaagggacg
       61 tggtcgcttg cgaccatgcg gagcgcgcgg agaagaggaa cgcggcgctc agggcaacca
      121 aaaacgaaat ctggaagatt cttttcacgg tctgcctccg gtggaatgca aatctgaaaa
      181 ctcgcgaaca agaatgccgg ggtgaaattc acgcgcaagc gcgtcttctt gggcgccaaa
      241 aatgtatgcg aacctaaagc tgaccaggac tgttcccctc gcccgccact cagactcacc
      301 ccgtggcaat ttgctggcag cgtagcgtgc aagcgatgta ccacaatttg gaagagaggg
      361 tcaagaaaaa gttaaaatgt gcaaattgat gggattgggt acgggcgttc gcgtatttgg
      421 cctgtccatc aaaagcagcc aatctaccta gaatggaata aatatacaaa atttggatgt
      481 ttatgcgttc agcggctaga gagagcagga cggggcttgg cggggaggca gtaagcttag
      541 aaggcgacca ggggccgcgt gatgatgcaa gaggcctcac gtcagctcgc gcaaaagcgg
      601 cgcgagcgag acgtgagcta caggggtgaa ccgccgtgaa cctgagggcc cacagtggcc
      661 cgacaaaaca cgtccggccc tacaaccgag gaactgttgt cggaacagct tggcgaaggc
      721 gaaaaaagag gcggccggtg gggggggccg gccgccgtgg gcatggggtg atggaggtgc
      781 aatcactttc gaggcccgaa tcgcgggggg ccacgccgaa cctcgaaggc gaggccattg
      841 tgccgggagc tggcgggcga gtcgataggc cggaaggttc aattagttgt ggtacgaatg
      901 tactttggca gcagaagggc cactgaatgt atatctataa aaatgaataa gttaggatga
      961 aatgaagaga attgccgact tcactcgtag cgcagggctt cgatggggtc aagcttcgcg
     1021 gctttgacgg cgggccagac gccgaaaacc agcccgatgc tgacggagac cacgatgccg
     1081 acgattacgg cccagagggg cacggaggcc ctcatcgaag tgatgtgagg aatgagcatg
     1141 gccaatgcgc tgaaaaaagc gacgccgagg atgccgcccg caccggtgag cgtcatggct
     1201 tcgaagagga actgccaagt gatgtcgcgg ctgcgcgcgc ctatggcttt gcggacgccg
     1261 atttcacggg tgcgttcggt gacgctgaca agcatgatgt tcatcacgcc cacgccgccg
     1321 atgagcaggc caattgagct aagcacgacg agcacgaggg cgacgccgcc aatgatttgg
     1381 ttgaactgct cctcctgctg aacctgcgtg gtgatggaga aattgtcggg cgcgcgatag
     1441 ggcactttgc gttcgcgacg caggacttca gtggcttcat cgacggcctc gtcaactttg
     1501 ccgtggacgg cgaggaaacg gtagccgtta tcttggaccg acggaaacat cttctggtac
     1561 gtgtaatacg gaatcatgac gcggatgtcc tggtcgttca ggccaaagcc ccccggcggc
     1621 ttggaaaaca cgccgatgac gaggaaatcg gaaccgtcga tggcgatggt tttctgaagc
     1681 gcatcctggg gcgtgggaaa taatgccttc gcggtatcct cgccgagaac gactactttt
     1741 gagcggtgct cgttttcggc ttcgttgaaa aagcggccgg ccagcaattg agcgttgcca
     1801 tagacttcca tgtaggcagg aaacgttccg cgaaaatcga tgttctgcgc ctgattcctg
     1861 ccgtatttga tgagatggat ctggaagcgg ttgaaaatcg tgatggcgat ttgcttcacg
     1921 tcggggcagg aggcgagaat cgcctggcct tgttcaagag tgagcggagg acgctcgcgg
     1981 gcttgtttca ggctgcgtcc cgcgcccgga ccgttgtcga agcggttgac gtaagcggtg
     2041 tccgcgccga acccttcgat gatttcgttg atgctggctt tgaatccggt gattagcgag
     2101 gcgacgataa tgattacgcc cacgccgatg accacgccga gcacagctag caagctgcgc
     2161 gttttgtgcg tgcgaagcgt gtcgaaagct aacaccagat tttcgcgaat gtcttgtttc
     2221 atcgagtttc tctaggcctc ggcgcgcagg gcttcgattg ggtcaagccg ggaggcgcgg
     2281 atagctggat aaattccgaa aaacaagccc acgcccgtcg agagcgccag cgcaatgata
     2341 accgcgtgca acggcgtaga gatcggcata ggcgacgcgg cgcgcacgag catggcaatt
     2401 ccaaacgcgg ccagaattcc cattgcgccc ccggtggcag caagaatgga cgattccatc
     2461 ataaattgga gcacaatgtg ctttctgcga gcgccaaggg atttgcgcaa tccgatctcg
     2521 cgggttcgtt cggtgacgct ggcgagcatg atgttcataa tgacgatgcc gcccaccaca
     2581 agaaaaacgc tggtcagcca gatggcgatt ccaaagatgt tgccggtcag actctgccac
     2641 aacccggtaa tagacgaggg catgatgatc ccgaaattgt cgggatcctg atagcggaca
     2701 tgccgccgcg cgcgcagcat gacgcgaact tcatcttcgg cgatgggcat ggcctccgta
     2761 gtaacggctt ccacgaaaat cgttactgag tcatttgggc cctgaaaaac gctcatgtag
     2821 gtggtaagcg gcagcatcac aaagttgtcc tgcgtttgac cgagaagagt gcccagcgcc
     2881 ttggcaacac caatgacctg gcactgctgc gcgccaacac ggatttcgcg gccgagcggg
     2941 tccacgccgg gaaacaggtt tacctgcacg tcgtggccaa tgaaacaggt cggagtgcgg
     3001 tgggtctcgt ccacatcggt gaaaaagcgg cccgcgacga catcgaaatc gcgaacgacg
     3061 gcgaactccg gagtggcgcc gatgacggtc acatcttcga taagcttttc accatagtgc
     3121 acgtcctgca ccgtattttc ttcggcggcg acttcgctgc tgtattggag gtggcctttt
     3181 agggcgtagt aatcgtccat atgcaaaggc gggcggcgca tggccttcac gaagtcgtca
     3241 aaattggtga tgattccgac gcggtctatt acgaacgcat tggcgccaag attggcgatc
     3301 tttgtggcca cataaagatt aagcccgttg agcacgctca tcacgctgac cagcgtagtg
     3361 acggcaagaa tcacgcccaa taacgtcaaa aaacttcgca gtttgtgcgc gcgcagcgta
     3421 tcgaatgcca gcgcggtggc ctcgcgaaga gcgatcatcg tctggcgaaa gccaagcttc
     3481 agtttgggat ctttttgcat ctactcagta tagacgtttt gacaacaata aaagttcact
     3541 gcgcaggtgc ggtaactctc tgaaccactt aaccacgcag cggagaggct tttgacgtca
     3601 agtgcgctaa aattagaacc agcaagattc tgctgaaatt gctgagccat tcgcaagaac
     3661 gaacgctaga aattcgccca tgaaaggcag atctccaatc cgactcgcgg ccattgcgct
     3721 cgcgggaatt ctgtgcggca tccagccgtg tgccacagcg caaatgcaag gtcagccaca
     3781 atcgccaccg ctggaccaaa ctccactcac tgccaagcaa ttggacgacc tcatttcgcg
     3841 caccatagca aatcagcacc gcaacgatgc ggcggccgac tccttcgagc gcatcgagca
     3901 ccacgtgtct cactcgggca gcgccgacgg acccgtgact caggacaaga cgtatcgcgt
     3961 cgtacctact ggcagcggca cgttaaagct gctgattcgg gacagctcca agaacgtttc
     4021 cgacgctttc tatcgccacc agctccgcga ctgggagaac attctcaaag tcgctgtgca
     4081 tcccgatgat ccccgccagg tcgccgtgat ggccaagcag cagaagcggc aaaaagaccg
     4141 cgcccgtttc atcgacgccg cgaatggcgc ctataaaatc agatggctgg cgcgtgaaat
     4201 ccgcgacggg cgcatcgtcg agaagctgca actcgatcca aatcccgctt ataagccacg
     4261 cggcgatgct accgattggc tgatgcacgc gcaggccatc atctggatcg accctcaaga
     4321 agcccaagcg gccgccatcg atgccactat cacccgcgac atcagtatcg gcggcggctt
     4381 cctcggcaaa atctaccgag gcggccattt tctgatgcag caggcccccg tcgcgtcggg
     4441 catctgggag cccacacatt atgaatacga tatctcgggc agaaagtttc tttttggatt
     4501 cacgatgcac gaggaaattt cggattcgca ttaccggctg ctcgggccgc cccagcagct
     4561 tctcgccgaa gcccagaaca gcctcgcggc ctgttgcgat atcgacgcgc taacgatggg
     4621 ggctactttg catagcggcg gcggacctca ctgaaaactg tttcgaaaaa accgggcggc
     4681 gcttcaggaa tcgcgttcgg atcataggtt tccatccctt taatgatttc gttcgcggga
     4741 agccggcgta ggaagtagac gtggggccac ggctcgctag gcgctttgcg ctcttcgccc
     4801 attacctgga agaattcgtc ctgatagcga atcgtcagcg ggtacttcca gttctccttt
     4861 ggccggaagg aagagacctc caagtcgcag caagtccctc ccataatcac gcgatcggct
     4921 acagcttttc ctcgaacgcc tgctttgttg ctgcgccacg aaagcaacca cagcggcagc
     4981 gttccgtagc tctcgccatt cactaccgcg gcgagcagtc gcacagttcc ttcacagccg
     5041 aaatagcaaa tcagccaggt cagcggatgg atggccgtcg aaaacaaacc tacgtacccg
     5101 ccaaactgcc ctccggctcc gatgatttcg ccaatgcgcg ttacatacat cgagtaccac
     5161 accacgagcg cgacgaatgc cgcgaaccct tccagcaggc ctgaaattgc ggcggctctg
     5221 ggccagtcca ttttccagtc gcagaaatag gcatctctcc aggccggagg caaaaaagcc
     5281 aggaatggtc cgatcaggta ggttcgcatc gtgtttagcc caatttagga gcatcccgat
     5341 ggcgcgccaa gcgctttgaa atgaggagcg gcgaaaaagc catcaacctt cgcttggaga
     5401 ccgctttact cggatggcgc gagaagcgta ggctatcggc atcgtttcgc agaggttccc
     5461 gccccgggga ggaaacatcc atgtacgcgc gcgtgacgca attccatctt ggccctgaca
     5521 aactcgagga atttgttgac gctgttcgcg ctgctatccc tcatgcgcac cagcagaaag
     5581 ggttccgcgc gctgctggta ttgcgggacg atgcgggcgc aacctccgcg catgtgcgtg
     5641 tgatttcggt ttggaactcg gccgaggatc ttcaagccgc cgagcagaat ttttatcttt
     5701 atcaggcgct ggcgaaagtg atgaccgcaa cgaaaggttt tccgctgatt gaaggccagg
     5761 aagtgctttt ggctgagctt gacagctaac attgcaagag aaaataccgc ttaaggattc
     5821 gggcttccga ggagtttgaa agaattttcc accattcttg accgcgaatc gcgcggccag
     5881 taagctagat gacttccaag cggcgcgtgc aagttttcgt gtcacctaat agtggcttgg
     5941 aattcttggc ggtatctgac caacatgagc accattcaaa tcacgctccc ggacggagcc
     6001 aaacgcgaag ttaacagcgg cactacgccc gcgcaaatcg cgcggcaaat ctcgccgcgc
     6061 ctggaaaagg aggcgctggt ggcgcgcgtg gatggacaac tgtgggacct ttcgcgtccg
     6121 ctcgacgaga gcacttcgct cgcgattctt acgcccaaag atccggaagc aattcaggtg
     6181 ctgcggcatt cagccgcgca tttaatggcc gccgccgtca tcgaactttt cccaaacgtg
     6241 aagctcggca ttggtccgcc catcgacaac ggatttttct acgagttcct gcgcgaagag
     6301 ccgttcactc cagaggatct ggaacgcatc gagaaaaaaa tgcacgagct cgcgtcgaaa
     6361 gacattccta acgagcgcaa gctcctgccc aaacccgaag ctctcaagct ctaccgcgag
     6421 tcgaatcagg aattcaaatg cgagctcgtt gaggaaaaag cgatcgagcc gatggtgtcg
     6481 ttctacatca ccggaaaatt catcgatttc tgccgcgggc cgcacattcc ttccaccgga
     6541 cgaatccgcg ctttcaagct gaccaacgtc gcgggcgctt actggaaagg acaagaaagc
     6601 aatccgcaga tgcagcggat ttacgcgct
//
LOCUS       DATNDT010000303         5191 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_653201, whole genome shotgun sequence.
ACCESSION   DATNDT010000303 DATNDT010000000
VERSION     DATNDT010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653201"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VN788_09840"
     CDS             complement(<1..747)
                     /locus_tag="VN788_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU20869.1"
                     /translation="MLIPLTLLVGSSVLCGGPAQKSGDEHGNSSDLIQAHVRAGLAFI
                     DRGSVEAAIREFQEAALLEPNNPEIHLYLGAAFRRAKDLSGAAAEYETSLKLQHENAE
                     AHSGLGHVLEDRGDLDGAVKQFRRAVQLSPRSPEHLNDLGAALAAAGKQAEAIAEFNK
                     TLAVDPKFCKARSNLGVALQEEKQLNAAIKAFRAALVCDPASEETHYIFGLLLRDTGD
                     LDGAIAELRAAVKLNPNDSRPRYNLALALNK"
     gene            complement(807..4037)
                     /locus_tag="VN788_09845"
     CDS             complement(807..4037)
                     /locus_tag="VN788_09845"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU20870.1"
                     /translation="MTRRWSSLRWVFLALMLGCGCVYGQLTLGTITGRTMDPQGSAVA
                     DCSVTVTNTETGAVRTTHSDGEGVYSVPALVAGSYRVTVSKTGFQDEAATVRLAINQV
                     LTQDFPLVIGTVTSTVNVSGSASEVALQKETHELGNVVSRMEIASLPVNGRNFISLAT
                     LGPGQQSASDAAGGTGTYFGTSDHQVLANGQPSTSTSFLQDGVDNLALFYYAANVVPD
                     LDAIQEFSIETNGMSAKFSQPSVVNAISKSGTNAFHGFGYDYLQNEDLNANNWFSNFY
                     GQPRGTNRFNQFGGGVGGPIKKDKTFFFFDYEGQRDTTTSPYSGRIPTLAERQGDFSD
                     YLAGVPTAVGEIQKQVIYDPNTFNQVTGEIKPFPGNIIPADRLSSFATLFNKFFPTPA
                     STISPDGSNYRTTLRSTNSIDNYLGRGDQYISSNDRLYGLFLSSSTPASSPTFTPGIF
                     GRSYDRSGINAALEETHVISPNAINTARVGYNRSIFFLSQIGAGTKNWLQEFGLKTLN
                     PTIEQNSPPGVSINGCCGLGDIFAPQGARQNRYQFSDEINYLHGKHQLFAGVDVVRNQ
                     FNGDWALANNGSLTFNGQFTSNHAAGSAFANGLGLADYELGLPSSAIGAEGNTVGAFR
                     ETDTAAYIQDNWRVRTNLVLNLGFRYQYSSPPADKYGRAGTFDLATATAVPGPWDPNY
                     KNFAPRVGLAYSLNSKTVIRSGYGIYYTSTPYNVFQFSLANPPNFLSQSFGFDLDHPT
                     PVSSLFPPITPGSSILSPFVLDKHNPTPYVEQWNFNIQRSLPSDMLLTVAYVGNASHH
                     LSVRSNPNQPSPDPDPLHPTSAQTRRPFPALGDVLAQYDQGNAHYNALQVSLQKHFSS
                     GFSFLLSYTYSKAMDMISGDGGQLYNGANPKAYYAVSDGDRTHIFTASYIYELPFGPG
                     KRYLDRQDFTSKYLLGGWQINGITTLETGLPFSVTAPDQSPGFTGSTFADRVCDGNLS
                     GDQRSMTKWFDTSCFQVPAFGRLGNSGRNILRMDGVRNWNVSLFKNTPLAHEGYTLQF
                     RAEAFNVFNQHDFLFGGQTGVTDAGFGQAQFSEAPRVLQLALKFLF"
     gene            complement(4233..5081)
                     /locus_tag="VN788_09850"
     CDS             complement(4233..5081)
                     /locus_tag="VN788_09850"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXU20871.1"
                     /translation="MRLAAADYSFPLLEWRQAAELVKNLGFRGIDISVFEGRSQLPAS
                     EVLANPSEYGARVRQVLDDIGLEIADVFAIPGSSFEQGAPNDPSALERAKSKDYFYRL
                     LEFTLRGNAKHLTLLPGIHFPQETVADSLKRCAEELEWRAVASRELGVRFSIEAHVGS
                     VVSTPALAAKLLDMAPSLTLTLDPSHFVSRGVPDAEILPLATRTSHFHARCGRAGRLQ
                     APLKQNTIPFDSYVKALHAVKYTGWFAVEYVWIDWESCNEVDILSETILMRNLLRTAE
                     NDLVNA"
BASE COUNT         1188 a         1454 c         1390 g         1159 t
ORIGIN      
        1 tttgttaagg gccagcgcca ggttgtaacg tgggcgcgag tcgttcgggt tcagttttac
       61 agcggctcga agctcggcga tagcgccatc gaggtctccg gtatcccgca gcaacagtcc
      121 aaaaatatag tgggtttctt cgctggcggg atcgcagact aacgccgcgc ggaacgcctt
      181 aatcgcggca ttcaattgct tctcctcctg cagggcgact ccgagattac tgcgcgcctt
      241 acagaatttg ggatcgacgg ccaaggtctt attgaactcc gcaattgcct cagcctgctt
      301 tcccgccgcg gccagcgccg ctcccaaatc gttgaggtgt tccggcgatc gcggcgataa
      361 ctggaccgcc cgccgaaatt gctttacggc tccatccaga tctcccctgt cttcgagcac
      421 atgacccaga ccgctgtgcg cttccgcgtt ttcatgctgc aatttgaggc tcgtttcata
      481 ctccgccgcc gctccggaca aatctttcgc ccgcctgaac gcggcgccca aatacaaatg
      541 aatttccgga ttatttggct ccaacaatgc ggcttcctgg aactcccgga tggccgcttc
      601 cacagagccc cgatcgatga aggcaagccc ggcacgaacg tgagcctgga ttagatctga
      661 gctattccca tgctcatctc cggatttctg cgcgggtccg ccgcacagaa cgctcgatcc
      721 cactagcaac gtcaggggta tcagcatccg gctccccgtc cagcggaatg ttggcaatgg
      781 ctgcaatcaa atacgaatct aacggctcag aacaggaact tcagcgctag ttgcaacact
      841 ctcggggcct cactgaactg cgcctgtcca aaaccggcat ccgtcacgcc cgtctggccg
      901 ccgaacagga aatcatgctg gttaaacacg ttgaatgcct cggctcgaaa ctgcagcgta
      961 tatccttcgt gagccagcgg cgtgtttttg aacaacgaca cattccaatt ccgaacgcca
     1021 tccatgcgga gaatgtttcg gcccgaattt ccgagccggc caaaggcagg cacctggaaa
     1081 cacgatgtat cgaaccactt tgtcatgctc cgttggtccc cggatagatt cccatcacaa
     1141 acacgatcgg cgaacgtgga gcctgtgaat ccaggagact ggtcgggcgc cgtgacactg
     1201 aacggcagac cagtctcgag ggtcgtgatc ccattaatct gccagccacc gagcaggtat
     1261 ttacttgtaa agtcctgtcg atccagataa cgctttcccg gcccaaacgg cagttcatat
     1321 atgtagctcg cggtaaagat gtgggttcgg tcgccatccg acaccgcata atacgccttg
     1381 ggattggcgc cgttatatag ttgtccgccg tcccccgaaa tcatatccat cgccttcgaa
     1441 tatgtatagc tgagcaagaa ggaaaacccg gaagagaagt gcttttgcag cgatacctgc
     1501 agagcgttat agtgcgcatt tccctgatcg tattgcgcca aaacatcacc caacgccgga
     1561 aatgggcgac gggtctgagc ggacgtggga tgtagcgggt ccggatccgg actgggttgg
     1621 ttgggattac tacgaaccga taagtgatgc gatgcatttc cgacgtaggc gaccgtcaag
     1681 agcatgtcgg agggcaatga acgttggata ttgaaattcc attgctccac atacggggtc
     1741 ggattgtgct tatcgagcac gaatggggac aggatgctgg acccgggcgt aattggcgga
     1801 aacaggctcg acaccggagt gggatgatcg aggtcgaatc caaagctctg cgatagaaaa
     1861 ttcggcggat tcgcgaggct gaactggaaa acgttgtaag gcgtcgaagt gtaatagatt
     1921 ccgtaacccg accggatcac agtcttgcta ttgagactat atgccaagcc aacacgcggg
     1981 gcaaaattct tataattcgg atcccacggt cccggcactg ccgtcgctgt cgccaggtca
     2041 aatgttccag cacgcccgta tttatccgcg ggtggggaac tgtactgata ccggaagcca
     2101 agattgagaa ccaggttcgt ccgtactctc cagttatcct gaatatatgc ggccgtatct
     2161 gtctcacgga atgcgccgac ggtgttcccc tcggctccga tggccgaact cggaagccct
     2221 aactcataat ctgcaagacc cagtccgttg gcgaatgctg agccggccgc gtggttggat
     2281 gtgaactgtc cattaaaggt aagcgaccca ttgtttgcca gcgcccaatc tccattgaac
     2341 tggttacgca ccacatccac acctgcgaac aactggtgct taccatgcaa ataattaatc
     2401 tcatcggaaa actgatagcg gttctggcgc gccccctgtg gagcgaagat atcgccaaga
     2461 ccgcaacatc cgttgatgct tactcccgga gggctgttct gctcgatggt cggattcagc
     2521 gtcttcagcc caaactcctg taaccaattc tttgtccccg ccccgatctg actaagaaag
     2581 aatatggagc ggttatatcc cacgcgggcc gtgttaatgg catttggcga gattacatgc
     2641 gtttcctcca atgcggcatt gatgccagat cgatcatagg acctgccgaa aattcccggc
     2701 gtgaaagtgg gactagaggc tggcgtgctg gaagatagga ataggccata cagacggtcg
     2761 ttcgatgaaa tgtactgatc gccgcgtccc aggtaattat caatgctgtt ggtcgaacgc
     2821 aacgtggttc tgtaattcga tccatcggga gagatcgtgc tggcgggcgt cggaaagaac
     2881 ttattgaaca gagtggcaaa ggaagacagc cggtccgcgg gaataatatt gccggggaac
     2941 ggctttatct ctccagtcac ctgattgaac gtattcggat cgtagatgac ctgcttttga
     3001 atctcgccga ccgcggtcgg aacgccggcc agatagtcgg aaaaatcacc ctgtctttcc
     3061 gcaagcgtcg ggattcttcc cgagtaggga ctagtcgtcg tgtcgcgctg cccttcatag
     3121 tcgaagaaga agaaagtctt gtcttttttt attgggccgc caacgccacc gccgaattga
     3181 ttgaagcgat tcgttccacg cggctgtcca taaaaatttg aaaaccagtt gtttgcattc
     3241 aggtcttcat tttgcaggta atcgtaacca aagccgtgaa aggcgtttgt accgcttttg
     3301 gaaattgcat tcactacact gggctggctg aatttcgccg acatgccgtt ggtctcgatg
     3361 ctgaattcct ggatggcatc gagatccggg accacgttag cggcgtagta gaagagcgcc
     3421 agattgtcga cgccgtcctg gagaaacgat gtcgaagtgg agggctggcc attggcaagc
     3481 acctggtgat ccgaagttcc gaaataggtg cctgttcctc cggcggcgtc cgatgcggac
     3541 tgttgacctg gtccgagcgt ggctaatgag ataaagtttc ggccattcac cggcagcgaa
     3601 gcaatttcca ttcgggaaac gacattgccc agctcatggg tctccttttg cagagccact
     3661 tccgaggcgc tgccgcttac gttcaccgta ctggtcaccg tgccgatgac cagtggaaaa
     3721 tcctgcgtga gtacctgatt gatcgccagc ctgacggtgg cggcctcgtc ttgaaaacct
     3781 gtcttggaga ccgtcacgcg atacgagcca gctaccagcg caggcactga gtacacgccc
     3841 tcgccatcgc tatgcgttgt tcttacggcg cctgtttccg tgttggtgac cgttacgcta
     3901 cagtccgcaa ccgcgcttcc ctgcgggtcc atcgttcggc cggtgatggt ccccagggtg
     3961 agctggccat aaacacaacc gcatccaagc atcagggcta aaaacaccca ccgtaagcta
     4021 ctccatctcc gagtcattgc gattcctctc aagaatgtga tctgacgcac gaggcctcca
     4081 actctcgttt gttgccctaa gcgctccgcg gcatcccgaa cgtgtatggc gacaaactac
     4141 gaccagcaaa cgtggcccca tcataatcgt cagttatggt tcctgtcaag aaaaaagttt
     4201 cttattaagg cggtttggat ttccctgggt gactacgcgt ttaccagatc gttttccgct
     4261 gttcgaagca agttccgcat gagaatcgtt tcactaagga tatcgacttc gttgcacgat
     4321 tcccaatcga tccagacata ctcgaccgcg aaccagcccg tgtatttgac ggcatgaaga
     4381 gccttcacat aggagtcgaa cggaatcgta ttctgcttca gcggcgcctg caatcgcccg
     4441 gcacgtccgc aacgcgcgtg aaaatggctg gttcgcgtgg cgagcggtaa aatctccgcg
     4501 tcgggaactc ctcgcgatac gaagtggctg ggatcgagcg tcaaggtcag cgacggcgcc
     4561 atgtcgagta gcttcgccgc caaagccggt gtcgaaacaa cggaacccac gtgagcttca
     4621 atcgagaaac gcacacccag ttccctactg gcgaccgcgc gccattccaa ctcctccgcg
     4681 caacgcttga gagagtcagc cacggtttcc tgagggaaat ggatcccagg cagaagcgta
     4741 agatgtttcg cattaccccg aagcgtgaat tccagcaggc ggtaaaagta gtccttggat
     4801 ttcgcccgct ccagcgccga aggatcgtta ggagctccct gttcgaacga gcttccggga
     4861 atcgcaaaga catcggcgat ctcaaggcca atatcatcca atacctggcg cacccgtgcg
     4921 ccatactcgg aaggattcgc cagaacctca ctcgcgggaa gctgcgaccg gccttcaaaa
     4981 accgagatat ctatccctcg aaatcccagg ttcttgacca gttcggcggc ttgccgccat
     5041 tcgagaaggg gaaagctgta gtcagcggcg gcaaggcgca caatggtttg agcctagcac
     5101 gacctatatt tctttccaag catcttgttg cataatatcc agcggtccag taacgttaga
     5161 tcgatgatga cacgcgttgt atcgtgagca a
//
LOCUS       DATNDT010000304         2199 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2439449, whole genome shotgun sequence.
ACCESSION   DATNDT010000304 DATNDT010000000
VERSION     DATNDT010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2199
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2439449"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="VN788_09855"
     CDS             complement(<1..542)
                     /locus_tag="VN788_09855"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712204.1"
                     /note="produces ATP from ADP in the presence of a proton
                     gradient across the membrane; the alpha chain is a
                     catalytic subunit; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HXU20872.1"
                     /translation="MANIKADEITQLLREQIENYQQTMAVEEVGSVISVGDGIARIHG
                     LESCMAGEMIEFPHGVFGIALNLEEDQVGAVLLGDYTEIVEGDTVKRTKQIMSVPVGD
                     ALIGRVVNPLGQPLDGKGPVTTDHRNPLERLAPGVVDRQPVREPLQTGIKAIDSMIPI
                     GRGQRELIIGDRQTGKTAIIL"
     gene            complement(552..1091)
                     /gene="atpH"
                     /locus_tag="VN788_09860"
     CDS             complement(552..1091)
                     /gene="atpH"
                     /locus_tag="VN788_09860"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HXU20873.1"
                     /translation="MNGAVSSRYAAALADVALKHDAGERIKADLGSFVAAFAASADLR
                     NFLETPAIGHEAKQNVIHKLAERMELAPEVRNFIQLLVLHRRTALLREIQQAFESDLN
                     ARMGIAEADVKSARELSAKDKEQLTAALEQRTGKKIQARFGEDRSLLGGLVVRVGSTI
                     YDGSLREQLARLRHKLEAE"
     gene            complement(1088..1675)
                     /locus_tag="VN788_09865"
     CDS             complement(1088..1675)
                     /locus_tag="VN788_09865"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20874.1"
                     /translation="MRRAKPHLFDLGVRLSTLVLLAAGSALGQENQPSGANSATGWLF
                     RWINFGIISALIIYAFLKASPGFRSRREELSRQIQEGARARETAERELREVQTKMASI
                     GTLTAELRVEAKRAAETEAQRLRELAKTEAGMVEEAAKAEIAAAERTAVLELRAFAAR
                     KAIERAEAMLQGEMTSDAEAALFGTFVTELHRSTN"
     gene            complement(1672..>2199)
                     /locus_tag="VN788_09870"
     CDS             complement(1672..>2199)
                     /locus_tag="VN788_09870"
                     /inference="COORDINATES: protein motif:HMM:NF012645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit B"
                     /protein_id="HXU20875.1"
                     /translation="HPLAGRQRAQGSGRNTLSSTMEILHKLGELFLQSLPTVALVLLF
                     YCFLRWAFFAPIQKAMSERHERIQGARTEAAAVEGTARQELDRYNETLRKAHAEIYGE
                     QEAARQVALESRSKLLKAMRARAQEDVDAAKQRIAAELGAARAAVESQAPSVAAEIVR
                     RMLETPSSPHTGTAR"
BASE COUNT          412 a          676 c          615 g          496 t
ORIGIN      
        1 agaataatcg cagtcttgcc ggtctggcga tcaccgataa tcaattcacg ctggccgcgg
       61 ccaatgggaa tcatcgaatc gatggccttg atgcccgttt ggaggggctc tcgaaccggc
      121 tgacgatcga ccacgccagg cgccaatcgc tccagtggat tgcggtgatc cgtggttacg
      181 gggcccttgc catcaagggg ctgcccgagc ggattcacga cgcgtccgat cagcgcatcc
      241 ccgaccggca cggacatgat ttgcttggtg cgcttcaccg tgtcgccctc gacgatttcc
      301 gtatagtcgc ccaggaggac ggcgcccacc tgatcttctt caaggttgag agcgatgccg
      361 aatacgccat gaggaaactc aatcatttca ccggccatgc aactctccag gccgtgaatg
      421 cgcgcgattc cgtcgccaac ggagatgacc gaaccaactt cctcgacggc catggtctgt
      481 tggtagttct caatctgttc gcgtaaaagc tgagtaatct cgtccgcttt gatattcgcc
      541 atttctggct cctattccgc ttccagtttg tgtcgcagcc gagcgagttg ctcgcgtaaa
      601 gaaccgtcgt agatcgtgga accgacgcgt actacgaggc ctccaagcag cgaccgatct
      661 tcgccgaatc gagcttggat tttcttgccg gtgcgctgtt cgagcgcagc ggtcaattgt
      721 tccttatcct tggcgctcag ctcccgtgcc gatttcacat ctgcttcggc gatgcccatt
      781 cgggcgttca ggtcagattc aaacgcctgt tggatttcgc gaagcaaggc tgtacgacga
      841 tgaagcacga gcagttggat gaaattgcgg acttctggcg caagttccat ccgctcggcg
      901 agtttgtgaa tcacgttttg tttcgcttcg tgccctattg caggtgtttc gagaaaattt
      961 cggaggtcag ccgatgccgc gaaggctgct acaaaactgc cgagatcagc cttgatccgt
     1021 tcgccggcat cgtgtttcag cgcgacatcc gccaacgccg ctgcatatct cgacgaaacg
     1081 gcgccgttca attcgtactc cggtggagtt ccgtgacaaa ggtgccgaag agcgccgcct
     1141 cggcatccga tgtcatttca ccctgcaaca tagcttcggc gcgctcaatg gcttttcgcg
     1201 cggcaaaggc tcgcaattcc aacaccgccg tgcgttctgc cgcagcgatc tccgcctttg
     1261 cggcttcctc caccataccg gcctccgttt tcgccagttc gcgcaagcgt tgcgcctcgg
     1321 tttcggcggc ccgttttgct tcgacgcgca attcagcggt aagcgtaccg atgctcgcca
     1381 tcttggtttg aacttcacgc agttcccgct cagcggtttc gcgggcccgt gccccttctt
     1441 gtatctgccg ggaaagttct tccctgcggc tgcggaaccc aggagatgct ttcagaaacg
     1501 catagatgat aagcgccgat attattccaa aattgatcca gcgaaagagc caacccgtcg
     1561 ccgaattggc tccggaaggc tgattttcct ggcccagcgc acttcccgcc gccagaagga
     1621 caagggtgct caggcgcact cccagatcga acaaatgcgg tttggcgcgt ctcacctggc
     1681 tgtccctgtg tgcggcgaag agggtgtttc gagcatcctg cggacaattt ctgcggcgac
     1741 cgacggcgct tgagactcga ctgcggcgcg agcggccccg agctccgccg caattcgttg
     1801 ctttgcggca tccacgtctt cctgcgcgcg cgcacgcatt gccttgagaa gcttcgagcg
     1861 gctctccagg gccacttgcc gggcagcttc ttgttcccca tagatttccg catgcgcttt
     1921 ccggagcgtc tcgttatagc gatcaagctc ctgccgagcg gtcccctcga ccgcggccgc
     1981 ttcagtccgg gcgccttgaa tccgctcgtg gcgctcggac atcgctttct ggattggcgc
     2041 gaagaaagcc catcgcaaga agcaataaaa caaaagaaca agggcgaccg taggcaggga
     2101 ttgaaggaac agctcaccga gcttgtgcaa aatttccatt gtcgaggaaa gggtgttgcg
     2161 gccgctcccc tgcgcgcgct gtctgcccgc cagcggatg
//
LOCUS       DATNDT010000305         5936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3080274, whole genome shotgun sequence.
ACCESSION   DATNDT010000305 DATNDT010000000
VERSION     DATNDT010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3080274"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2611
                     /locus_tag="VN788_09875"
     CDS             <1..2611
                     /locus_tag="VN788_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative maltokinase"
                     /protein_id="HXU20876.1"
                     /translation="EIDADYSCRALLAEANQWPTDVRPYFGDGDECHMAFHFPLMPRI
                     YMALRQEDRLPITDIIAQTPAIPDTCQWALFLRNHDELTLEMVTSDERDYMYLAYSAD
                     PRMRINLGIRRRLAPLVDNNRRRIELLNSLLFSFPGTPIIYYGDEIGMGDNIYLGDRN
                     SVRTPMQWNGDRNAGFSTATPARLYSPVIMDPVWGYEAINVESQQGDPSSLLNWMRNM
                     VALRKLFRVFGRGAIEFLDPPNRKVLAYLRQYEGEQVLCVANLSRFAQPVDLDLSKLE
                     GAVPVEMLGYVEFPTVGRHPYRLTLAPYSFLWLELQRRPETEESAVPPYEIPLEITIG
                     WRSVFEGPGLLRLETESLLEYLPKQRWFAGKTRRLKSTRILDSVVLEKSGSALALVEA
                     QFESGAPDIYQLMFAMAFGEAAEELRRTSPNAVITGIVSGEESGILHDGVFNDQACAE
                     LLSLMKNASELALPHGRIRSVRGKAFQDLMPSDGQYLAVRRGSAEQSNTSILFDDRFI
                     LKLFRRQDVGVNPDCEVGRYLTEKANFDRVPAFAGSIEYESKDGQNLTLVMLQGLVAN
                     EGDGWKWTVEELDRYYENCATATLPDENLGDDLATPLELSAQPSSQAAREHVGLYLDS
                     AATLGKRTAEMHLALASSTADPAFSPEPFTSEDLQSLVKALQDHAASVFDVLKERVSS
                     LPDELLEVAASVLSRRRRILQHFATFSSRDSGSPLPQRIRIHGDYHLGQVLRVKTDFV
                     ILDFEGEPARSLAYRRSKQCALKDVAGMLRSFSYAAYATLINYTARRPQDLATLQPWA
                     RFWERSVAAEFLRAYRQAAQGADFLPADESDFQNLLDAFLLDKALYEVLYELNSRPAW
                     VRIPLMGIMSLSL"
     gene            2679..3926
                     /locus_tag="VN788_09880"
     CDS             2679..3926
                     /locus_tag="VN788_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20877.1"
                     /translation="MQTEPVAPQVPEKTEQPDTADFVVGILSDLPQQGIAQLCSALQT
                     APGAPKIVILATQAVGESVRNDVGTHSASVSIASWPPVSADVVAGPLQSVSAAYHSAF
                     SISERLGARGCCVIASQLQIDAPRWVNEFAQPLLESQCDLVVPSYGHQKFQGLLNSGI
                     MCPLTRSLYGKRIQNPVGPDLGVSQKLLQKIIASNRASRNDATITHPLISLTPSSACD
                     NLRISQVYAGSRTYPPADWTNVSSILVQVLGPVFLEMEKRAPCWQKVRGSISVPTLGP
                     HVSVSTEEVAPDVTRMIESFQLGVRDLQEIWGLVLPPATLLELRKLSRLEPQQFRMKD
                     ELWARIVYDFALAHRLRTINRDHLLRSMAPLYLAWVASYSNEIKSAGSAAVDQRLERL
                     CLAYEAAKSYLVSRWRWPDRFNP"
     gene            3949..4653
                     /locus_tag="VN788_09885"
     CDS             3949..4653
                     /locus_tag="VN788_09885"
                     /inference="COORDINATES: protein motif:HMM:NF017373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20878.1"
                     /translation="MWYQVKQALDQSTQLFLTRLANLLPGIVALIVALVVALILAAII
                     AAVVRRLLVRMNFDERLVQWGFASVFLWSPMKSPTLLVSRVLAALVIFCGFLIGLAAF
                     DPDLTSVLARTVFAYIPNIVGALIVLAAGSIIARFLARSVLIGAVNMNFQYGRLLSVG
                     VKWLVIVLTVAMALEHLRISAGIVELAFGILFGGVVLVFALIIGMNSKELLAKSLERD
                     GKKPSAETVEDPLRHL"
     gene            complement(4708..5766)
                     /locus_tag="VN788_09890"
     CDS             complement(4708..5766)
                     /locus_tag="VN788_09890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20879.1"
                     /translation="MIFFVAPSGETWELEDYLKSYGKELLARASVLTWDQIVARQELG
                     LGSYVFLAIDQMSPTEKEIAAQCWEKLSQARSDITLINHPSTVLLRYDLLQTCYERKR
                     NAFRVRRGLDFLRCDKFPVFLRREHDHNASISGLLSTRKELVRAIARGLYLGFRLRDL
                     IIVEYCDTADGAGMFRKYSSFIVGDRVLPHTLMHSDQWITKSHGRLVDAATAREELEY
                     VQKNPHGEWVRETFSLANIRYGRIDYGLLDGKPQVWEINTNPTIVRRPEAGPIPEQQR
                     RMRDPVRQVFFPSLQSALEAIDSVADPSQTVPIEISQSQRRKLDDEKRQRVRVQAKRT
                     FISRRAGPVFRSLRRLLS"
BASE COUNT         1190 a         1729 c         1629 g         1388 t
ORIGIN      
        1 cgaaatcgac gccgattatt cctgccgagc gctcttagcc gaagccaatc agtggcccac
       61 ggacgtgcgg ccttatttcg gcgatggcga tgagtgtcac atggcgtttc attttccgct
      121 gatgccgcgc atttacatgg ccctgcgcca ggaagaccgc cttccgatta ccgacatcat
      181 cgcccaaact cccgcgattc ccgatacgtg ccagtgggcc ctttttctga gaaatcatga
      241 cgagctcact ctcgagatgg tcaccagcga cgaacgcgac tacatgtacc tggcctacag
      301 cgccgatccg aggatgcgca ttaacctcgg catccgccgc cgcctcgctc ctctcgtcga
      361 taacaatcga cggcgcatcg agttgctcaa cagcttgctc ttttccttcc ccgggactcc
      421 gatcatttat tacggtgatg aaatcggcat gggagacaac atctatctcg gcgaccgcaa
      481 ctctgtgcgt acgccaatgc agtggaacgg cgaccgcaac gcgggctttt ctactgcgac
      541 tcccgcgcgg ctctacagcc ccgtaatcat ggatcccgtt tggggctacg aagcaatcaa
      601 cgtggaaagc cagcagggcg atccatcttc gcttctgaac tggatgcgca atatggtcgc
      661 gctgagaaaa cttttccggg tgttcgggcg cggcgccatc gaattcctgg accctcccaa
      721 tcgcaaggtg ctggcctacc tccggcaata cgagggagaa caggttttgt gcgtggcgaa
      781 tctctctcgg ttcgcgcagc ccgtggatct tgatctctcg aaactcgaag gcgcggttcc
      841 cgtcgaaatg ctcggctacg tggaatttcc aaccgtcggg cggcatcctt atcgtctgac
      901 tctggctcct tacagttttc tttggctcga acttcagcgc aggcccgaga ccgaagagtc
      961 cgctgttccc ccgtacgaaa ttccgcttga gattacgatt ggatggcgga gtgttttcga
     1021 aggtccggga cttctacgcc ttgagacgga aagccttctc gaatacttgc caaagcagcg
     1081 gtggttcgct ggaaaaacaa ggcgactgaa gtccactcgg attttagatt ctgtagtact
     1141 tgaaaaatcc ggatcggcgt tggcgcttgt ggaggcgcag ttcgaaagcg gcgctccgga
     1201 catctatcaa ttgatgtttg cgatggcatt cggcgaggcc gctgaggagt tgcggcgcac
     1261 ctctcccaat gcagtcatca ctggaattgt ttccggcgag gaatcgggaa ttctgcacga
     1321 cggagtcttc aatgatcagg cgtgtgccga gttgctttct ttgatgaaga atgcctcgga
     1381 actggcgctt ccgcacggac ggatcagaag cgtacgcggc aaggcatttc aagatttgat
     1441 gccgtccgat ggtcaatacc tcgccgtgcg gcgcgggtcg gcggagcaga gcaatacttc
     1501 cattctgttc gacgaccgct tcatcctgaa gctctttcgc cgccaggatg tcggcgtcaa
     1561 tcccgattgt gaagtcggca gatatctcac ggagaaagcc aacttcgata gggttccggc
     1621 cttcgccggc tccatcgagt acgaatcgaa agatggccag aacttgacgc tcgtgatgct
     1681 gcaaggtctg gttgcgaatg aaggcgatgg ctggaagtgg accgttgaag agctcgatcg
     1741 ctattacgaa aattgcgcca cggccactct tcccgacgag aacttaggcg atgatctcgc
     1801 caccccgctg gagctttcag cgcaaccgtc atctcaggcc gcaagggagc acgtgggtct
     1861 ttatcttgat tcggcggcca cgctcggcaa gcgtacggca gagatgcatt tggccttagc
     1921 ttcctccact gccgatccag ctttttcacc agaaccattt acgtccgagg acttacagtc
     1981 cctggtcaag gccctccaag atcatgccgc gtccgttttc gacgttttga aggaaagagt
     2041 ctccagcctg cccgacgagt tgctcgaggt cgcggcctcc gtgctcagcc ggcgccgccg
     2101 gatcctccaa catttcgcga cgttcagctc gcgcgattcc ggctctccgc ttccgcagcg
     2161 tattcggatc cacggagact atcacctcgg acaagtcctc cgggtcaaaa cggacttcgt
     2221 gattttggat tttgagggcg agccggcgcg ctccctcgct taccgcagat ccaagcagtg
     2281 cgcactcaag gacgttgccg gaatgctgcg atccttcagc tatgccgctt atgcgaccct
     2341 cattaactat acggctcgcc gtccccagga cttggcgaca cttcagccct gggcccgttt
     2401 ctgggagcgt tcggtagccg cggaattttt gcgcgcatat cggcaggcgg cccagggagc
     2461 tgattttctg ccggctgatg aatcggactt tcagaacttg ctcgacgcct tcctgttaga
     2521 taaagcgctc tatgaggtac tctacgagtt gaatagccga cccgcgtggg tgaggattcc
     2581 gctaatgggg atcatgtcgc tgtcgctgtg atatttctat cagagacgct tttacttcat
     2641 ccgatcgtta ggtgtccgtc gatatgagcg gttcagaaat gcaaaccgag cccgtcgcgc
     2701 cgcaagttcc ggaaaagacg gaacagccgg acaccgccga cttcgtggtc ggcattcttt
     2761 cggacttgcc tcagcagggc attgcccaac tatgtagcgc tttgcaaacg gctcccggcg
     2821 ctcccaagat tgtcattctt gcgacgcagg ctgtcggaga atcagttcgc aacgacgtcg
     2881 gaacacactc tgcatccgtt tctatcgctt cttggcctcc ggtgagtgcg gatgtcgtcg
     2941 ctgggccgct tcagagcgtt tcggccgcgt accactccgc tttttccatc agcgagcggc
     3001 taggcgcccg cggttgctgc gtgattgcct cgcaactcca aatcgacgcg ccgcggtggg
     3061 tgaatgaatt cgcccagccg ctcctcgaat cccagtgcga cctcgtcgtg ccctcctatg
     3121 gacaccagaa attccaaggc ttgctgaata gcggcatcat gtgccccctg acgcgctcac
     3181 tgtacggaaa gcgaattcag aatcccgtcg gccccgattt aggagtttct caaaagctcc
     3241 ttcagaaaat tatcgcttcc aatcgggcat ccaggaacga tgcaaccatc actcatcctt
     3301 tgatttccct cactccttca tctgcctgcg acaatctccg catcagccag gtgtacgccg
     3361 gatcgcgcac atatcctccc gcggactgga cgaacgttag ttcgattctg gtccaggttc
     3421 tgggacccgt gtttctggaa atggaaaaga gggcgccctg ctggcagaag gtacggggct
     3481 ccatttctgt tcctacgctt ggtccgcacg taagcgtttc aacggaagag gtagcgcctg
     3541 acgtgactcg catgattgag tcattccagt tgggagttcg agaccttcag gagatttggg
     3601 gcttggtgct tcctcccgcc actctgctcg agcttcggaa gctttctcgg ctggagccgc
     3661 agcaattccg catgaaggac gagctgtggg cgcgcatcgt gtacgacttc gcgctcgcgc
     3721 accgtcttcg aaccatcaat cgcgatcatc tgcttcgttc catggcgcct ttatacctag
     3781 catgggtcgc ttcgtattct aacgagatta aatcggccgg aagtgccgcc gttgaccagc
     3841 ggctcgaacg tctctgcctc gcttacgaag cggctaaatc ttatctagtc tcccggtggc
     3901 ggtggccgga tcgcttcaat ccgtaagcgg agataaacgg aggaacgtat gtggtatcaa
     3961 gtcaagcagg cgctcgatca atcgacccaa ctattcctta cgaggctcgc gaacctgctt
     4021 ccgggaattg tcgcactcat cgtggctctg gtggttgcgc tgatcctggc cgccatcatt
     4081 gcggcggtgg ttcgaagact tctggtaaga atgaatttcg acgagcggct ggtgcaatgg
     4141 ggattcgcct cggtcttttt gtggtcgccc atgaagagcc cgacgctcct ggtttcgcga
     4201 gtgctcgcgg cgctggttat tttctgcggc ttcctgatcg gtctcgctgc cttcgatccg
     4261 gacttgacgt ccgtgctggc gcgcaccgtc ttcgcttaca ttccgaatat cgttggcgcc
     4321 ctgattgtgc tggccgccgg cagcattatc gccagattcc tcgcgcgcag cgtgctgatt
     4381 ggcgccgtca acatgaattt tcaatatgga cgcttgctga gcgtgggcgt gaagtggctg
     4441 gtgatcgtgc tcacggtggc aatggccctc gaacatcttc gaatcagcgc gggcattgtc
     4501 gaattggctt tcggcatcct gttcggcgga gttgtcctgg tatttgcgct gatcattggc
     4561 atgaattcga aggaactgct agctaagtcg ctcgagcgcg atggcaagaa gccctcggcc
     4621 gaaacagttg aggacccgct ccgccatcta tagctgcttc cacttagtaa acgagtccgg
     4681 ctactcgatc ctacgtttaa cgccaattta gctcagaagg cgccgaagcg aacggaatac
     4741 tgggccggca cggcgagaaa tgaaggttct tttcgcttga actcgcactc gctgtcgctt
     4801 ttcgtcatcg agcttccggc gctggctttg cgaaatttcg atggggaccg tttggctcgg
     4861 atccgccacg ctgtcaatcg cttctaaagc agactgaagg cttgggaaaa aaacttgccg
     4921 caccggatcg cgcatccgcc gttgctgctc cgggatcgga ccggcctcag ggcggcggac
     4981 aatggttgga ttcgtattga tctcccaaac ctgcggtttc ccatcgagta atccgtaatc
     5041 gatgcgccca tagcgtatat tcgccaggga gaaggtttcc cgtacccatt cgccgtgcgg
     5101 atttttctgc acgtactcga gttcttcgcg agccgtagcc gcgtcaacca ggcgtccgtg
     5161 cgacttcgta atccattgat cgctatgcat caaagtatgc ggcaaaactc tgtcgccaac
     5221 gatgaacgac gagtacttcc gaaacatccc cgctccgtct gccgtgtcgc agtactccac
     5281 aataatcaaa tcgcgaaggc gaaaacccag atacaagcct cgtgcaatcg cgcgaaccaa
     5341 ttccttgcgc gtggaaagca agcccgagat gctggcgttg tgatcatgtt cgcgtcgcaa
     5401 gaaaactgga aacttgtcgc agcggagaaa gtccaaccct cgccgaacgc gaaaagcatt
     5461 ccgtttgcgt tcgtagcagg tctgcagcaa gtcgtaccgc agtaacaccg tcgagggatg
     5521 gtttatcagt gtgatgtccg atcgcgcttg ggagagtttc tcccagcatt gggcggcgat
     5581 ttccttttca gtgggggaca tttgatcgat cgccagaaac acgtagcttc caaggcccaa
     5641 ctcttgccgc gccacgatct ggtcccaagt caaaacgctt gcccgagcga gcagttcctt
     5701 cccgtacgac ttgagatagt cttcgagctc ccaggtttcg cccgaagggg cgacaaagaa
     5761 aatcatcgat ctcttcctgc gccgaatagc ctgcgcgtct tccgagaagt atagtgaggc
     5821 ggatgtttct taattgacag cacgaggatg tctcgactcc gcgaccgcca ccgaaccgca
     5881 ctacactggg catatccagt caagatgtgc catattaccg cgatcctttc ggcagg
//
LOCUS       DATNDT010000306        12321 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2900328, whole genome shotgun sequence.
ACCESSION   DATNDT010000306 DATNDT010000000
VERSION     DATNDT010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12321
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2900328"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            189..755
                     /locus_tag="VN788_09895"
     CDS             189..755
                     /locus_tag="VN788_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU20880.1"
                     /translation="MPSDQQITTRIAPKPRSKDVTELLKRAQSIMKEERWRDAIKLLK
                     NNRALVERHWELLWNLGWCYFKLERLGDARKFLTKAAKLAPESHNCRFGLGAVYLEMK
                     QFSKAERILSEALQIRESHIYRLLLAFAYLAQGKIEQAESTHLTNTKLRPKRSEAYEA
                     YADFLSDVGREGDAEEMYRKAKELRTLN"
     gene            complement(1511..3769)
                     /gene="glgB"
                     /locus_tag="VN788_09900"
     CDS             complement(1511..3769)
                     /gene="glgB"
                     /locus_tag="VN788_09900"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012911760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXU20881.1"
                     /translation="MSETARPAEPSEIVDPFEVELNLLVKGRLPDPYQVLGPHWVERE
                     GRPALAIRAFLPRAVKVSITWGTEPHQTAKIHPDGIFEAILPLEATRQGSGSDVRPNA
                     YRIRWVSGDRYETETYDPYAFPPVLTDYDLHLSAEGTHYQQYEKFGAHIREVDGVRGV
                     HFAVWAPNAQRVSVVGDFDSWDGRVHCMRNRGSTGIWEMFMPGLDQGQVYKYEILSRV
                     GDHLALKSDPYGFCAEMRPNTASVVWDIDRHQWQDNEWMYKRASRDWLHSPMSIYEIH
                     AGSWRRKASEGYSWLTYHELADELIPYIKQMGYTHVELMPIMEHPFDGSWGYQTIGYF
                     AVTSRFGTPSDFMYFVDRCHQEGIGVILDWTPAHFPRDAHGLAFFDGTHLYEHEDPRK
                     GAHPDWGTLVFNYSRTEVQNFLISNALFWIGKYHVDGLRVDAVASMLYLDYSRKAGEW
                     IPNAFGGRENLDAINLMKRLNEVLHAQHPGALMIAEESTSWPMVSRPTYLGGLGYDLK
                     WNMGWMNDTLRYFALDPIHRQYHHGELTFSMLYAFHENFVLPLSHDEVVHGKRALLEK
                     MPGDDWQKFANLRLLFGYMYAHPGKKLLFMGGELAQRHEFWDGGQVQWELEDSPWHKG
                     VQNLIGDLNRLHTGERAMHEVDFEWDGFEWLETHDSANSVLAFVRKALNPEDFVVVVC
                     NFTPVARENYRVGVPKAGYYREIMNTDSQFYAGTDFGNSGGIQAEPIPWSDRPYSLKL
                     RLPPLAAVYFKL"
     gene            3990..6491
                     /locus_tag="VN788_09905"
     CDS             3990..6491
                     /locus_tag="VN788_09905"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU20882.1"
                     /translation="MSSRIVTLRPKRKEKPCPVTSPRPRVEEQPLHSIRPNLAKPLPE
                     VAADLRFRQLFDQAFEGMFQTTPDGRYLRVNHTLARMYGYSNAQELMSAVSDIGAAVY
                     VDPRKREEFKRLMEEKGRIESFEYQVRDRYGRKIWLREKAWAIRDNLGRIVSYDGAVQ
                     DITERKRSDLERRVTTEILRAMTATGDLDELLKLIHSALKEVVSAENCFVSLYNESTS
                     LFEFPFFVDEFDSAFEPEAAGRSCMDYIFRTGKPDLISPQRLKELVKQGEVELVGTPS
                     KSWLGVPLKTPSATIGVLIVQDYKAENAYSERDLEFLDSIGGQIALAIERTRSEQRLH
                     SSDTRLRLLIEQLPAVLWTVDKDMRFTSALGAGLARLSLKPNELVGMKLADYLQTSDG
                     TYPAVAAHRRALAGEPVTFHLEWKSGSYACHAEPLRGPQGEIQGVICMALDVTDRKQL
                     EEQFRQAQKMEAVGRLAGGIAHDFNNLLMVIQGYADLLIERLHASDPLRKNVEQIQTA
                     SQRATSLTRQLLAFSRKQMLAPKVLNIQAVIADMETILRRLIGEDIVLEAVTVPSLGH
                     VKADRSQIEQVVMNLAINARDAMPSGGRLTIEAQNVEIGSDVPQQPSVVAPGKYVMLA
                     VTDNGCGMNEETRAHIFEPFFTTKEKGKGTGLGLATVYGIVKQSGGYIWVYSEPGVGT
                     TFKIYLPRIDEEEARSGADGDADSCALARGSEVVLLVEDEKGVRELTREYLETSGYSV
                     VEASDGYSALELARAHDGPIHALITDVVMPGLSGRELSEQMAKLRPGIKVLYMSGYTD
                     QSAFRNGILHKGAELLQKPFGMSTLASKLKEILAN"
     gene            6742..7044
                     /locus_tag="VN788_09910"
     CDS             6742..7044
                     /locus_tag="VN788_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20883.1"
                     /translation="MQVATQGTLTNEKDSGEIRVTDSVGERRLWLAVVMQAVEEWRNG
                     TLRNRRLAQQFLFDDAKDFQAVCANAGLDADNLRSRLLKMGRKVNAAGPLLRPLAA"
     gene            7260..8438
                     /locus_tag="VN788_09915"
     CDS             7260..8438
                     /locus_tag="VN788_09915"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HXU20884.1"
                     /translation="MPAATYEAVSEKRLLIACARTQMTAAAAQQIREITSRPLDWDYL
                     LRAAAENSILPLLALHLPAVASDAFSPEQSARLKAAARAAGIRALQLSAELIRVVDGL
                     RAEGVLALPYKGPVIAVQAYGDLALREFEDLDIIVPQRDMARANDVLLDLKYSPKFPW
                     IFGQSAIVPGEYNYYDRERRIIVELHTELTLRHFPVVPKLQEMSRRVENVTIGGHAVP
                     TFGAEDTLVLLCIHGSKDFWERISWIADVSEFVQREKRLDWPRIFLRAEELRATRMLK
                     LGLALASRLLDARLNGEVRARVDADGTALALAKQIEQRLLAREVLPRNVTQSFRYRRQ
                     MVPGALCSWRYGLRLATAPAEEDWNMAPLPRALSPLYVVLRPFRLFRKYGVSRESRRE
                     "
     gene            complement(8449..9834)
                     /locus_tag="VN788_09920"
     CDS             complement(8449..9834)
                     /locus_tag="VN788_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXU20885.1"
                     /translation="MAAASSTTIKARPKTPPGPKGHFLLGNLAAVSHDWLGFYSRCAK
                     DYGDIVHLRYLHIPICLLMHPRDIEYVLVTNPGNFTKSADYRALARALGKGLLTNEGK
                     SWQHQRSLIQPAFRRDNILSYAPVMTRIASRSLASWSDGQSRNVHEDMMAMTLEIVAQ
                     CLFGAEVSGIAERVGNAMQVVTDRFITDASQALLLPFQLPNFLAPARQRAIRDLNTII
                     DEIIRERRASKQPRADLLGTLLDVRDADGRPMSDTQLRDEVMTLFLAGHETTAIALSW
                     TCYLLAKNPQVEAMLVEELRDVLGGREPMVEDIPRLRYTEMILKESMRLYPAVWGIGR
                     RAIRDCEIGGYRVAAGTNIFIFQALTQRDPRFFANPDEFNPERWREDPVRSGTIPRFA
                     YFPFGGGPRVCVGASFAMIEATLLLATIQQKFHLALVPGHPVEPVPSVTLRPKHGIRV
                     TVHRREFAATA"
     gene            complement(10170..10943)
                     /gene="modA"
                     /locus_tag="VN788_09925"
     CDS             complement(10170..10943)
                     /gene="modA"
                     /locus_tag="VN788_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HXU20886.1"
                     /translation="MRRGLLLVLLITALSASACATRAASPQKSSIIVSAAASLKDALD
                     AITPAFAKDQPNIAIHYNLGASGTLQQQIQQGAPVDVYISASPAQMDSLDKQGLLLSG
                     TRRNLLRNTLVLIVPAGSSQVSDFKDLLKPGVRIVAIGEPRTVPAGKYAQEVLTHFGL
                     YLSLTPKFVLAKDVRQVLTYVASGNADAGIVYATDAKITNQVTVTATAPEDSHAPIVY
                     PVAVLKDTKSVEAAKSFVAFLISPAAQSIFEKYGFSPAE"
     gene            complement(11430..11843)
                     /locus_tag="VN788_09930"
     CDS             complement(11430..11843)
                     /locus_tag="VN788_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HXU20887.1"
                     /translation="MSESKLFTPREAAQVMGISYPTLKQWIYKKKLQTVQTAGGHHRI
                     PESEVDKYLFRSMEKHDVAEKRSGFRRVSGRNQLIGRVTDIKIDGLIAQITLSIGGQK
                     ITSIITADAVREMRLKKGDTAAALIKSTEVMILRP"
BASE COUNT         2787 a         3260 c         3576 g         2698 t
ORIGIN      
        1 ccactctggc tcatccgcta gccaactcaa gcgcaagcat ttgcctgctc gtatttttat
       61 cgaaactatg aagcgatcag cagaaccaga ttcctggtga cgaagtgccg tactagaccg
      121 gtatttgtcc gggtaggcga cttcacagga gctgacgccg gtgaaggcgt gatagattcg
      181 gtttcatcat gccatcggac cagcaaatca caacgcgaat cgcaccgaaa cccagatcaa
      241 aggatgtaac cgaactcctc aaacgcgctc aatccataat gaaagaagaa cgatggcgtg
      301 atgcgatcaa gctcctcaaa aacaaccgtg cgttggtcga aagacactgg gagcttttgt
      361 ggaatcttgg gtggtgctat ttcaaattgg aacggctggg tgacgcccga aaatttctaa
      421 cgaaggccgc gaagctggca ccagagagtc acaactgcag atttgggtta ggcgcggttt
      481 atctagaaat gaagcaattc agtaaagcgg aacggattct ttccgaagca ctccaaatca
      541 gagagagcca tatctaccga cttctgctcg cgttcgctta tttggctcaa ggcaaaattg
      601 agcaggcaga gagcacgcac cttacaaata caaagcttcg gcccaagaga agtgaagcat
      661 atgaagcata tgcagatttt ctttctgacg tcgggcgtga aggtgacgcg gaggaaatgt
      721 atcggaaggc taaagaatta cggactctaa actaactctg actgttcaat acgccttcgc
      781 gtcggaacct acgcgttgag ccgagacagg acattgtgcg cgcgagaacc cacccactca
      841 aaccgcatga tgagttgaga gaaaatcctt gcacgctttg ctcccgtaga tatccggggg
      901 ccgtcgcaag atttgcggcc caaggccccg gcgatccagc caagcggtaa gactccgaga
      961 tttcagtgcc atattggttg agcaactctt cgacgggcat cgtctctaaa tcgaaaacga
     1021 tgtttccgaa cgcatcattc tcaaggtgat agccttcaac gcctgtgaaa attgcgttta
     1081 caaattctat cggtcgattt gccgcgcgat tttcagtacg gagtgtgatc gtccgggcct
     1141 cgcactggac ctcataagag accaagaaat tgtcgtgaag ggatagagcc attcttccgc
     1201 tgtgtccgga aaacagtttc tcagactacg ctcgtagggc ggatgagcca agtcccgtgt
     1261 aacggcgtaa ccggacaagt gcagtactgc aactatccct gacacctcgc cctctcacca
     1321 ttgagaccga gtcctcagaa atggaaaaaa ttttccgttg ctctttttct cgcgattaac
     1381 cgaattcaaa ttacagcggc attcgcgcca aattaggctg cataagctca tttaagtgca
     1441 caaacgcgca aagttgagta tgtcgttgca gcagaaaatg cacgcctgaa atacctgtcg
     1501 gtggggttgt ttaaagcttg aaataaactg ccgctaatgg cgggagccgt agcttgaggg
     1561 aatacggccg gtcgctccag ggaatcggct cggcctgtat gccgccggaa ttgccgaaat
     1621 ccgttcccgc atagaattgc gagtccgtgt tcatgatctc gcggtaatag ccggctttcg
     1681 gaacgcccac gcgataattc tcccgcgcca ccggcgtgaa gttgcacacg acgacaacaa
     1741 agtcttctgg attcagcgcc ttgcggacga atgccagcac gctattcgcc gagtcgtgcg
     1801 tctcgagcca ttcgaagccg tcccactcga aatccacttc atgcatggcg cgctcgcccg
     1861 tatgcagacg attcaaatca ccgatcagat tctgcacgcc tttgtgccac ggcgaatcct
     1921 caagctccca ttggacttgt ccgccatccc agaactcatg tcgctgcgcc aattcgccgc
     1981 ccatgaaaag cagtttcttg cccggatgcg cgtacatgta tccgaaaagc agtcgcaggt
     2041 tcgcaaactt ctgccagtcg tcacccggca ttttttccag caacgcgcgc ttgccgtgca
     2101 caacttcgtc atgcgaaagc ggcaaaacaa aattctcatg gaacgcgtag agcattgaaa
     2161 acgtgagctc tccgtgatgg tattgccggt gaatcggatc aagcgcaaag tagcgcagcg
     2221 tgtcgttcat ccaccccata ttccacttca ggtcgtagcc aagtccgcca agatacgtcg
     2281 gtcgcgaaac catcggccat gatgtggatt cttccgcaat catcagcgcg cccggatgct
     2341 gtgcgtgcag cacctcattc agccgtttca tgaggttgat tgcatccaaa ttttcgcgcc
     2401 cgccaaacgc gttgggaatc cactccccgg ctttccgcga atagtcgagg taaagcattg
     2461 acgcaacggc atctacacgc aagccatcca cgtgatattt cccgatccag aagagcgcgt
     2521 ttgagataag gaagttctgc acctcggtcc ggctgtagtt gaaaacgagc gttccccaat
     2581 ccggatgcgc tcccttgcga ggatcttcgt gctcgtaaag gtgcgttcca tcaaaaaacg
     2641 ccagcccgtg cgcgtcgcgt ggaaaatgcg cgggcgtcca gtcgaggatc acgccgattc
     2701 cttcttggtg gcaacggtcc acaaagtaca taaagtcgct cggcgttccg aagcgactcg
     2761 tcaccgcaaa atagccgatc gtctggtagc cccacgaacc atcgaaagga tgctccatga
     2821 tcggcatcag ctcgacgtgc gtgtaaccca tttgttttat gtagggtatg agttcgtcag
     2881 cgagctcgtg ataggtaagc cagctgtagc cttcgcttgc tttccgtcgc cacgatccgg
     2941 catgaatttc gtagatagac atgggtgaat gcagccagtc acgcgatgct cgcttgtaca
     3001 tccactcatt gtcctgccac tgatgccggt cgatatccca aaccacggac gcagtgttcg
     3061 ggcgcatctc cgcgcagaat ccgtaagggt cgctcttcaa cgcaaggtgg tcgccgaccc
     3121 gcgaaagtat ttcgtactta tagacttggc cttgatccag cccgggcatg aacatttccc
     3181 atatcccggt cgaacctcgg ttgcgcatgc aatggacgcg cccgtcccac gaatcaaaat
     3241 cgccaaccac gcttacgcgc tgcgcattcg gcgcccagac agcaaagtga acgccgcgca
     3301 caccatcgac ctcgcggata tgcgcgccga acttctcata ttgctgataa tgcgtgcctt
     3361 ccgcggaaag atggagatcg taatcggtca ggacgggagg gaaagcgtac ggatcgtatg
     3421 tctcagtttc gtagcgatcg ccgctaaccc atcgaatccg gtacgcattg ggccggacgt
     3481 cgctgccgga accttggcgc gtcgcctcta gcgggagaat cgcctcaaat atgccatcag
     3541 gatggatttt cgctgtttgg tgaggctcag tgccccaggt aatgctcact ttgactgcac
     3601 gtggcagaaa ggctcgtatc gcaagtgcgg ggcgcccttc gcgttccacc caatgcggac
     3661 ccagaacctg atatggatcg ggcagtctgc ctttgacaag caggtttaat tcgacttcaa
     3721 agggatcgac gatctcgctc ggttccgctg gcctggctgt ctcgctcatg ttcgtttccg
     3781 ctaaaggctg cggctaataa gcatattaca gcggcagttc cgcgcagggc ggagaaaatt
     3841 cggatatcga gtttgcgtca cgcgctccga gttagtacga acgtaccatt gaggtacggt
     3901 cttgctggta cagtagcata ttcgcggggt tgctctctcg accaaggatc taattttact
     3961 ctgtcgaaac tcttgctgtt ctaggactta tgtcgagccg gatcgtaacg cttcgtccca
     4021 agcgcaaaga aaagccgtgc ccggtaacgt cgccgcggcc tcgggtcgaa gaacaaccgc
     4081 tccactcaat cagaccgaac ttagcgaagc ctctccctga agttgcagcc gaccttcgct
     4141 ttcgtcagct cttcgatcaa gcgtttgagg gcatgtttca aaccacgccc gacggtcgct
     4201 atcttcgcgt aaatcatacg cttgcgcgca tgtacggcta ttcgaacgcg caggagttga
     4261 tgagcgcagt ctccgatatc ggcgcagcgg tttacgtaga tcctcggaaa agagaagaat
     4321 tcaagcgact gatggaagaa aagggccgca tcgagtcctt cgaatatcag gttcgcgacc
     4381 gttacggccg caaaatctgg ctccgcgaga aggcctgggc gatccgagac aacctcggtc
     4441 gcattgtcag ttatgacggt gctgtgcaag atatcaccga acgcaagcgt tccgacctgg
     4501 agcgccgcgt caccacggaa atccttcgcg caatgacggc gacgggcgat ctggacgagt
     4561 tgctgaaact gatccacagc gcactgaaag aagtggtttc cgcagaaaat tgcttcgtgt
     4621 cgctctacaa cgaatcgacg tccctatttg aatttccgtt ttttgtggat gagtttgatt
     4681 cggcattcga accggaagcg gcgggccgca gctgcatgga ttacattttc cggacgggca
     4741 aaccggacct tatatcgcct cagcgactga aggaattagt gaaacaaggc gaagtggaac
     4801 tggtcggaac acccagcaag tcttggttag gcgtaccttt gaagacgccg tctgccacca
     4861 tcggcgtcct catcgttcaa gattataaag ccgaaaatgc ttacagcgag cgcgatctcg
     4921 aattcctgga ctccatcggc gggcagatcg ccttggccat cgagcggacc cgctcagagc
     4981 aacgcctgca ctcaagcgac actcgcctcc gcctcctcat tgagcagctt cctgccgttc
     5041 tctggacagt tgacaaggat atgcgcttta cctcggcgtt gggggcaggc ctcgcgcgac
     5101 taagtcttaa gcccaacgag ctggtgggca tgaagctcgc cgactacttg cagacatccg
     5161 acggcacata tccggctgta gcggcgcatc gtcgagcatt agccggcgaa ccggttacat
     5221 tccatctgga gtggaagagt ggttcgtatg cgtgtcacgc cgagcctctt cgcggaccac
     5281 aaggcgaaat tcagggcgtt atctgcatgg cgctggacgt gaccgatcgc aagcagctcg
     5341 aagagcagtt ccgtcaggcc cagaaaatgg aagcggttgg acggttggct ggcggcatcg
     5401 cgcacgattt caataacctg ctgatggtca ttcagggata cgccgatttg ctcatcgaac
     5461 gcctacacgc aagcgaccct cttcgcaaaa acgtagagca gatacaaacc gcgtcgcagc
     5521 gcgccacttc acttacgaga cagctgctcg ctttcagccg caaacagatg ctcgcgccta
     5581 aggtgctgaa tattcaggcc gttatcgcag atatggagac gatcctacga cgattgattg
     5641 gcgaggacat tgtgctcgaa gccgtcaccg ttccgtcgtt gggccacgtg aaggcggacc
     5701 gcagccagat cgagcaagtg gtgatgaatc tcgcgattaa cgctcgagat gcgatgccca
     5761 gcggtgggcg gcttaccatc gaagcccaga acgtcgagat cggttcggat gtgccccagc
     5821 agccaagcgt tgtagctccg gggaagtatg tgatgctagc cgtaacggac aacggttgcg
     5881 gcatgaacga agaaacgcgt gcccatattt ttgaaccgtt cttcacaaca aaggagaaag
     5941 gcaaagggac agggctcgga cttgcgactg tctacggcat cgtgaagcag agtggcggct
     6001 acatttgggt ttatagcgag ccaggagtcg gcacgacctt caagatttat ttgccgcgga
     6061 ttgatgagga agaggctcgt tccggcgccg atggagatgc agattcgtgc gcgttggccc
     6121 gtggctccga agtcgtcctg ctagttgaag acgagaaggg cgtgcgcgaa ttgacacgcg
     6181 agtacctcga aacgagcgga tacagtgtgg tcgaagccag cgacggatat tccgcgctcg
     6241 aacttgcgcg cgcgcacgac ggtccaattc atgcgctgat tacggacgta gttatgccgg
     6301 gcttgagcgg ccgcgaactg agtgagcaga tggccaagtt aaggcccgga ataaaagtgc
     6361 tctacatgtc cggctatacg gatcagtctg ccttccgaaa tggcatcctc cacaaaggcg
     6421 ccgaactcct tcaaaaacca tttggcatgt ccacgcttgc ctcgaaactg aaagagatcc
     6481 tcgccaacta agtgtgcgca gtggctgtgc gtaatgggtc actaaggaac agttgcgcga
     6541 tctggagcaa tccttcctag tacctccttg ctccagagca actttgggtg tgatggatac
     6601 ggctctaact cttgcgctct cagtgttttc attgcctggc cctgatcgcg tatgtgaacg
     6661 agtctttggc gtatttcgtg cttttctctc tagcgaagca agagtgacag catctgcgaa
     6721 atccagctag gggggctcgg catgcaagtt gcaacacagg ggacgctgac gaacgagaag
     6781 gattcggggg agatcagggt tacggattcg gtcggggagc gcaggctgtg gctggccgtg
     6841 gtgatgcagg ccgtggagga gtggaggaat ggtacgttgc gcaaccggcg cttggcgcaa
     6901 caatttcttt ttgacgatgc aaaggacttt caggcggttt gtgcgaatgc ggggctcgac
     6961 gcggataacc tgcgttccag gctattgaaa atgggacgga aagttaacgc ggcgggaccg
     7021 ttgcttcgtc ctctggcggc ctgaaatcgt gcgttgccgc ggcgacgtcg ctggcatgtc
     7081 ttgcagcatc tgcgacagcc ggtatgacgc aaaaaggcaa aaatggcgcg ataaaatcgc
     7141 ctaaacgatc ccaaatggcg tgtttggcgc cttaaatcgg cagagagttt attttggaac
     7201 gatggcggag gaataggcgc tccggctgag gcatctgtcg taaaatcgac acgaatcgga
     7261 tgcctgccgc cacatatgag gctgtttctg agaagcggct gctgattgcg tgcgcccgca
     7321 cgcagatgac tgcggcggcc gcgcagcaaa tccgcgaaat aacctccagg cctcttgact
     7381 gggattacct gctgcgggcg gctgccgaaa actcgattct gccgcttttg gctttgcacc
     7441 ttccggctgt agcttccgat gcgttctctc ccgaacaatc tgcaagactg aaggctgccg
     7501 ctcgcgccgc gggtatacgt gcgctgcaac tctcggccga gttgatccgc gtcgtcgacg
     7561 ggttaagggc ggaaggcgtt ctcgcgctgc cttacaaagg ccctgtgatc gcggtgcagg
     7621 cgtatggcga cctggcgcta cgcgaattcg aggatctgga catcattgtt ccgcaacggg
     7681 acatggctcg tgccaatgat gtgttactcg acttgaagta cagcccgaag tttccgtgga
     7741 tattcgggca atcggctatc gtgcccggcg aatacaacta ttacgaccgt gagcggcgca
     7801 ttatcgtgga attgcacacc gagttgacgc tgcgtcactt tccggtcgtg ccgaagctgc
     7861 aggagatgag caggcgcgtg gaaaatgtga cgatcggcgg gcatgcggtg cccacgtttg
     7921 gtgcggaaga tacgctcgtt ctgctctgca ttcacggctc aaaggacttt tgggagcgaa
     7981 tttcttggat tgccgatgtt tcggaattcg ttcagagaga gaagcgcctg gattggccgc
     8041 gcatcttcct gcgcgcagaa gagttgaggg ctacgcgaat gctgaagctt ggcctcgctc
     8101 ttgcttcgcg actccttgat gcgcgcctga acggagaggt tcgcgcgcgc gtcgacgccg
     8161 atggcacagc tctcgcgctt gcgaagcaga ttgagcaacg gctactcgct cgggaagttt
     8221 tgcctcgcaa cgtcacccaa tcgtttcgct acagaaggca gatggttccc ggtgcactgt
     8281 gttcgtggcg ctacggttta cgtctggcga cggctccggc cgaggaggat tggaacatgg
     8341 cgccgctgcc gcgcgcgttg tcaccgcttt atgtggtgtt gcggccgttt cgcctttttc
     8401 gaaaatacgg agtatctcgc gaaagcagac gcgagtgagg aacgtacctc aggcagtggc
     8461 agcaaattcg cggcgatgga ccgtcacacg gatgccatgt tttggtctta atgtgacgga
     8521 gggaactggc tcaacaggat ggccaggcac cagggcgaga tgaaattttt gctggatcgt
     8581 ggcaaggagc agcgtggcct cgatcatggc gaacgaggcg cccacgcaca ctcttgggcc
     8641 gccaccaaac gggaaataag cgaatcgcgg aattgtcccc gagcgaactg gatcttcccg
     8701 ccaccgctcc ggattgaatt cgtccgggtt ggcgaagaaa cgcgggtcgc gctgcgttag
     8761 agcttggaag ataaaaatgt tcgtgcccgc agcaacacga tagccgccaa tttcgcaatc
     8821 ccgtatagcg cggcgtccaa tgccccaaac ggcgggatag agccgcatcg attccttaag
     8881 aatcatttcc gtatagcgga gacgcggaat atcttccacc ataggctcgc gaccgccgag
     8941 cacatcgcga agttcctcga cgagcatggc ctcaacctga ggattttttg cgagcaggta
     9001 gcaggtccaa gacagtgcga ttgccgtcgt ttcgtggccc gccagaaaga gtgtcatcac
     9061 ctcatcgcgc agttgagtgt cactcattgg ccgtccgtct gcatcacgca catcgagaag
     9121 cgtaccgagt aaatctgctc gcggctgctt cgaggcacgg cgttcgcgga taatctcgtc
     9181 gatgatcgta tttaaatcgc ggatggccct ttgccgtgcc ggcgcgagga aattgggaag
     9241 ctgaaaggga agcaggaggg cctggctggc atcggtgatg aacctatcgg ttacaacctg
     9301 catcgcattg ccgacgcgtt ccgcgatgcc gctgacttct gctccaaaaa ggcattgcgc
     9361 tacgatctcg agtgtcatag ccatcatgtc ttcgtgcacg ttgcgggatt ggccgtccga
     9421 ccaggaggcg agtgagcggc tggcgatgcg agtcattacc ggcgcgtagg acagaatatt
     9481 gtcccgccgg aaagcaggtt ggatcaatga tcgctggtgc tgccatgatt tcccttcatt
     9541 agtcagcaag cccttgccga gcgcacgcgc gagggcacga tagtcggcgg attttgtaaa
     9601 attgccggga ttcgtgacca agacgtattc gatatcacga gggtgcatca gcaggcagat
     9661 ggggatgtga agatagcgaa ggtgcacgat atccccgtag tccttggcgc agcgggagta
     9721 gaaccccagc cagtcgtgcg aaacagcggc gagattgcca agaaggaagt ggcctttggg
     9781 accgggcgga gtctttggac gagctttaat tgtggttgaa gaagctgccg ccatagcgtg
     9841 gtgtcctgcg ctgtctttta acttccaaaa aagaaatggt acagcaatgg ggagtgtccc
     9901 gcgggataaa ttgggcgggc cttcggtaaa aaacttcggg aacgctgctc agacggcagc
     9961 gggaggcctg tcgttttgcg acgaggtcac gcttcaagtg ccgcgctgaa ggctggcggc
    10021 gctaaaccaa aaatcaaaac cgaaagtcga aaaagagggc gaaacccgcc gatcagaaat
    10081 cgacattcgc gtgacaagca agacgcaggt acggagaccg gcgctcccgg gggaggcacc
    10141 gtcgaaggcg cgggccaacg gcacggcgtt tattcagccg gtgagaagcc gtacttttcg
    10201 aaaatggatt gcgcggcggg cgagatgagg aaagcgacga aggatttggc tgcttcgacg
    10261 cttttcgtgt ctttcagcac ggccacggga tagacaatgg gcgcgtggga atcttcggga
    10321 gcagttgcgg tgaccgtcac ttgattggtg atctttgcgt ccgtggcata gacgatgccg
    10381 gcgtcggcat ttcctgaggc gacgtatgtg agaacctgcc gaacatcctt cgccagcacg
    10441 aacttggggg tcagcgatag gtagagaccg aaatgggtga gcacttcctg cgcgtatttt
    10501 ccggcgggaa cggttctagg ctctccgatg gccacgattc ttacgcctgg cttgagcaag
    10561 tccttgaaat ctgaaacttg ggaagagcct gctgggacga tgaggacgag tgtgtttcgg
    10621 agcaggttgc ggcgcgtgcc ggaaagaagc aagccttgct tatcgagcga atccatctga
    10681 gcgggcgagg cggagatata aacgtcgacc ggagctcctt gttggatctg ctgctgcaag
    10741 gtcccggatg cgcctagatt gtagtggatc gcgatgtttg gctggtcttt tgcgaaggcc
    10801 ggggtgattg cgtcaagagc gtctttgagg ctggctgctg cggagacgat gatgctggat
    10861 ttttgaggag acgccgctcg agtcgcgcag gcggatgcag aaagcgcggt gataagcaga
    10921 accaaaagaa gtcctcggcg cattgctctc cgcaaagaca aagtggatta agccgtaaca
    10981 gaatggcggt tcaagttgat tataacgaga tttattggca ggcgatagct ccgcgcgggt
    11041 ttcgattggc gcgaagtcct agatttacgg ggaggcatgt gccgcgcctg cggcgctcta
    11101 ttctaatttt gtgctctccc agcgctcacg cgcttggtta agttgtgtcg cacctacggt
    11161 gctgattctc tgcgactggg aacctttttg gcgcggaaga gcatttcatc gaggccaaac
    11221 attgcagcta caatgcgatt tttcagcgaa ctgcgactcc cattgtaaat gccgctatat
    11281 ttcggaaccc ctcgttgcct cagagttctt gacgaaattc gcgacgtgac tgaagtcggc
    11341 ggaagacaaa gtaagaggga tccttcgccg cgcccttaga tacgggcgcg gctcaggatg
    11401 acgaattcgc gaagagaagc ggagagtgat tacgggcgga ggatcattac ttcggtggat
    11461 ttgattaatg cggcggcggt gtcgcctttt ttgaggcgca tttcgcggac ggcgtctgcc
    11521 gtgatgatcg atgtgatttt ctggccgccg atggagaggg tgatctgagc gatcaatccg
    11581 tcgatcttga tatccgtcac gcggccgatc agttgattgc ggccgctgac gcgacgaaat
    11641 ccggagcgct tttcggcaac gtcgtgcttt tccatggagc ggaagagata tttgtcgact
    11701 tcgctttcgg gaatgcggtg gtggccgccg gcggtctgga ctgtttgaag ctttttcttg
    11761 tagatccact gcttcagcgt ggggtagctg atgcccatga cctgggcggc ttctcgcgga
    11821 gtaaatagct tggattcgga catattcggg gcaagcttcc catcggcagg tttccttgta
    11881 aagcgatctt ttagcggtca tgactgaaaa tcggggatca aagccagggg tacgcgcatc
    11941 aaccaaacat gggcaccgcc gatacagaat tcgtgagaat cagtgagacc tagctcctcg
    12001 atgaatgagt atttacagga aacgccgcgc cgcatcccgt tcgttgcgtt agaatcgatc
    12061 tttacgttgc gcagtcgcgc gccatacata tctgcccggg aggaaggacg attccatccg
    12121 taaacagcgc caccacttta ccggaacaaa aaacgcaaaa cccgcttaag gagctgctct
    12181 cttacggcca gtccgtgtgg ctcgattaca tccggcgcaa cctttttacg acgggcgaat
    12241 tagtgcgtct ggtgaaggaa gatggattgc gcggcatgac gtccaatccg gcgattttcg
    12301 agaaggctat tgccggcagc a
//
LOCUS       DATNDT010000307         4295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3452127, whole genome shotgun sequence.
ACCESSION   DATNDT010000307 DATNDT010000000
VERSION     DATNDT010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4295
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3452127"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /gene="argC"
                     /locus_tag="VN788_09935"
     CDS             <1..653
                     /gene="argC"
                     /locus_tag="VN788_09935"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HXU20888.1"
                     /translation="ILAQAIYGLPELYADALTNARIVGNPGCYPTSVILGLRPLVEEG
                     WINPTRGIVCDCKSGVTGAGKEPKRDTQFVEVNQNFRAYNVFTHRHTPEVSEHLGLAS
                     ENFVFTTHLLPVDRGILSTLYVWLDRSRKPDEIEALYRKFYTGRQMVRLMPAGTLPEL
                     QHVTRTNFCDIGFALDHSGQRLVIVSCLDNLGKGAAGQAIQNLNGMRGYPETAGLL"
     gene            650..1477
                     /gene="argB"
                     /locus_tag="VN788_09940"
     CDS             650..1477
                     /gene="argB"
                     /locus_tag="VN788_09940"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HXU20889.1"
                     /translation="MRLVVKIAGALLERDEDVQALARQIAELARAGNEILVVHGGGKI
                     FTATLARMGITSRFISGLRVTDRETRDAAVMVFAGLLNKKLAGAISLAGQPAIGISAT
                     DGACFLAEPMQLEESGGALGFVGYLTEVNVDFLRSLWREGIVPVASCMGLGADGELYN
                     INADHMAAAAAEFIHADRLIFLTDVAGVLDGEKVLRAVRGAEIEDLIRRNKVSGGMIL
                     KLEAAKRALAGGVAEVRIVGGTQPRALVAAAGAMRGPGTRVLPVLRPSAKAAHGAAQ"
     gene            1479..2795
                     /locus_tag="VN788_09945"
     CDS             1479..2795
                     /locus_tag="VN788_09945"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXU20890.1"
                     /translation="MAKKKAGATKQVSAAKVVAEESRYVMRTYRRPPVVFTRGHGCGL
                     YDSEGCRYLDFLGGIAVNALGHAHPRMVRVIRREAGRATHISNLFHNPYQASLARKLS
                     EWSGLDRAFFSNSGSEAIEGALKLARAYAHARAASNTGQPKIRILALENSFHGRTMGA
                     VSITHTAKYREPFAPLIPGVEFVRFNDIEDLEAKFDGTVCAIVIEPIQGEGGVFLVSE
                     EFWERARAIATRHDAALIADEIQCGLGRTGRKFGYQKFPSLPDIVTVAKPLAGGLPLG
                     AFVASEKFASTFIPGMHGSTFGGGPLVCAAALEFLTIVEGEDLLANVRARGAELRAGL
                     EKLAQRFDFIDEIRGEGLILGIDLAVEGGPFVQEALRQKLLINCTHDHILRLLPPFIV
                     RQREVAEFLTKFATVLERGSKSAKKSLSRAPKAEVHTQPMALAATR"
     gene            2799..3743
                     /gene="argF"
                     /locus_tag="VN788_09950"
     CDS             2799..3743
                     /gene="argF"
                     /locus_tag="VN788_09950"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019688771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HXU20891.1"
                     /translation="METATTTLSLKRDLLTGTEWTPAQSRDCFRLASDMKAHPHRYHT
                     ALAGSVFVLIFEKPSLRTRVTFEVGIANLGGSSIFLDHTATRLGEREPIRDIARNLER
                     WVQGIVARVFDQESLEQLAKNANVPVINALSDRFHPCQALADFFTLEERFGGLRGLKF
                     AYIGDGNNMCHSLLVAGARCGAHVRVATPAGYEPDEAIVAEAKRVARETRGKIEIVRS
                     PEEAVAGVQAVYTDVWASMGQEDEAAEREAAFMGYQVNEVLFAQAAPDAVFLHCLPAH
                     RGLEVTGGVIDSPRSIIYDQAENRLHVQKAILHTLLLA"
     gene            3754..>4295
                     /locus_tag="VN788_09955"
     CDS             3754..>4295
                     /locus_tag="VN788_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HXU20892.1"
                     /translation="MPKKVVLAYSGGLDTSIIIPWLKENYGGCEVIAMIGDLGQREDL
                     EAARKKALATGASEAYVEDLREEFIKGYIWPALRAGAVYEHKYLLGTSFARPLLAKRQ
                     VEIAKKVGADAVAHGCTGKGNDQVRFELAAKALAPELTIIAPWREWKINSREEAIAYA
                     REHNIPVEQTTKNIYSRDRN"
BASE COUNT          886 a         1215 c         1312 g          882 t
ORIGIN      
        1 agatcttggc tcaggctata tacggtctcc ccgagcttta cgcggacgct ctaacaaacg
       61 cgcgcatcgt gggaaacccg gggtgctatc ccacgtcggt gattttgggg ctgaggcctc
      121 ttgtcgagga gggctggatc aacccaacgc gtggaatcgt ttgcgattgt aaatcaggtg
      181 ttacaggcgc gggcaaggaa ccaaagcgcg atacgcagtt tgtcgaagta aatcagaatt
      241 tccgcgctta caacgttttc acccatcgcc acacgcccga agtttccgag catctcggcc
      301 tggcctctga aaatttcgtc tttaccacgc atctgctgcc ggtcgatcgg gggattctct
      361 cgacgcttta cgtctggctc gatcgctcgc gcaagccgga tgaaatcgaa gccctttatc
      421 gcaagtttta tacggggcgg cagatggtca ggttgatgcc ggcgggaacg ctgcctgaat
      481 tgcagcatgt gacgcgtacg aatttctgcg acatcggctt cgcgctcgac cattcgggcc
      541 agcggttggt gattgtctcc tgcctcgata atctcggcaa aggagcagcg gggcaggcga
      601 tacaaaatct aaatgggatg cgcggctatc cggaaacggc gggattgcta tgagactggt
      661 cgtaaagatt gcaggtgcat tgcttgaacg cgatgaagac gtgcaggcgc tggcccggca
      721 gatcgctgag cttgcgcggg ccggtaacga gattttggtg gttcacggcg gcggtaagat
      781 tttcacggca accttggcac gcatgggaat cactagccgc ttcatcagtg gtctgcgcgt
      841 aaccgaccgt gagacgcgcg acgcggccgt gatggtattt gccggtcttc tgaataagaa
      901 gctggctgga gcgatctccc tcgcgggcca accggccatc ggcattagcg ccactgacgg
      961 cgcctgtttc ctcgcagaac ccatgcagct tgaggagtcc gggggcgctc tggggttcgt
     1021 cggctatctc acggaggtca acgtcgactt cttgcgttcc ctgtggcgtg agggaatcgt
     1081 gccggtggca tcctgcatgg gactcggcgc cgatggggag ctttacaaca ttaatgctga
     1141 tcatatggcc gccgcggcgg ccgaattcat acacgccgac cggttgatct tccttacgga
     1201 tgtggcgggt gtgctcgatg gcgaaaaggt gttgcgagcg gtgcgcggag cggagatcga
     1261 ggacctaatc cgccgaaaca aggtttccgg cggcatgatc ctgaagctcg aagcggcgaa
     1321 gcgtgcgcta gccggcggcg ttgcggaagt acggatcgta ggcgggacgc aaccgcgagc
     1381 gttagtcgcc gctgccggtg ctatgcgggg accaggcacg cgagttcttc cggtgctgcg
     1441 ccccagtgct aaagcggcgc acggagcggc gcaataacat ggccaagaaa aaggctggag
     1501 caacaaagca ggtctcggca gcaaaagttg ttgccgaaga gtctcgctac gtgatgagga
     1561 cgtatcggcg gccaccggtg gttttcacgc gcgggcacgg ctgcggcttg tacgattcgg
     1621 aaggctgcag atatctcgat ttcctcggcg gaatcgccgt caacgcactc ggccacgcgc
     1681 accctcgcat ggttcgagtg atcaggcgcg aggcgggccg cgcaacacac atttcgaatt
     1741 tattccataa cccctaccaa gcctcgctcg cgcgcaagct atcagagtgg tccggcctcg
     1801 accgcgcatt tttctcgaat agcggcagcg aagcaatcga aggcgcgctg aaactcgcac
     1861 gcgcatatgc gcacgcgcgc gcggcaagca acacgggcca accgaagatt cgcatcctgg
     1921 cacttgagaa ttcgtttcac ggccgcacga tgggggccgt ttccataacc cacaccgcga
     1981 agtaccgaga gccatttgcg ccgcttattc ccggagtgga attcgttcgg ttcaacgata
     2041 ttgaggatct ggaagcaaag tttgatggca cggtatgcgc cattgtcatc gagcccatcc
     2101 agggcgaagg cggcgtcttt ttggtcagcg aggaattttg ggaacgggcg cgggcaatcg
     2161 cgacacggca cgatgccgcg ctgatcgccg acgaaattca atgcggcttg ggccgcaccg
     2221 gccgcaagtt tggttaccag aaatttccgt cccttccgga catcgtcacg gttgccaagc
     2281 cgcttgccgg cggtcttccg ctcggcgcat ttgtcgccag cgagaaattc gcatccactt
     2341 ttattcccgg catgcacgga tcgacgtttg gtggcggccc gctcgtctgc gccgcggcgc
     2401 tcgaatttct gacgatcgtc gagggcgagg acctgcttgc gaatgttcga gcgcggggcg
     2461 cggaactgcg tgctggcctg gagaagctcg cgcaacgctt tgatttcatt gacgaaatac
     2521 gaggcgaagg attaatccta ggcattgatc tcgctgtcga aggcgggccg ttcgttcagg
     2581 aagccctccg gcaaaagctg ctgatcaatt gtacccacga ccacattctg cggttgctgc
     2641 cgccattcat cgttcgacag cgagaggtcg ccgaattcct gacaaaattt gcaaccgtgc
     2701 tcgaacgggg gtccaagtcc gcgaagaaat cgctttcccg cgctccgaag gctgaagtac
     2761 acacccagcc gatggccctg gctgcgacga ggtaagtcat ggaaactgcg accacaaccc
     2821 tttctctcaa gagagatctc ctgacgggaa ctgaatggac tcccgctcag tcgcgggatt
     2881 gcttccgcct tgcctcggat atgaaggccc acccacatcg gtaccacacg gcccttgccg
     2941 gcagcgtgtt cgtcctgatt ttcgaaaagc cgtcactgcg cacgcgcgtc acgttcgaag
     3001 tgggaattgc caatctcggc ggctcgtcga tctttctaga tcatacggca acgcgcctcg
     3061 gtgagcgtga gccgatccgc gatatagcga gaaatcttga gcggtgggtg caaggcatag
     3121 tcgcgcgtgt tttcgatcag gagtcgctcg agcagttggc caagaacgcg aacgttcccg
     3181 tgatcaatgc gctctcggat cgttttcatc cctgtcaggc gctggcggat ttctttaccc
     3241 ttgaagagcg cttcggcgga ttgcgcggcc tgaagtttgc ttatatcggg gatggcaaca
     3301 acatgtgcca ttctcttctc gtggccggcg ctcgctgcgg agcgcacgtt cgcgtcgcca
     3361 cgcccgccgg ttacgagcca gacgaggcga tcgttgccga agcaaagcga gtagctcgtg
     3421 aaacgcgcgg caaaattgaa atcgttcgct cgccggaaga ggccgtcgcc ggtgttcagg
     3481 ctgtttatac cgatgtatgg gccagcatgg ggcaggaaga cgaagccgcc gagcgcgaag
     3541 cggctttcat gggatatcag gtgaacgaag ttcttttcgc gcaggccgct ccggacgctg
     3601 tcttcctaca ttgtttgccg gcccaccgcg gcttggaagt tactggcggc gtcatcgact
     3661 cgccgcgttc catcatttat gaccaggcgg aaaaccgcct gcacgtgcag aaggcgattc
     3721 tgcacacgct attgctcgcc taaggagaat ccgttgccca agaaggtagt tttggcttat
     3781 tccggaggcc tcgatacatc cataattatt ccatggctga aggagaatta cggcggctgc
     3841 gaagtaattg ccatgatcgg cgatctgggc cagcgcgagg atctggaagc tgcgcgcaag
     3901 aaggctctcg ccacaggagc aagcgaagca tacgtcgaag atctgcgcga ggaattcatc
     3961 aaggggtaca tttggcccgc tcttcgcgcc ggagccgttt acgagcacaa gtatttgctc
     4021 ggcacctcat tcgcccggcc gctgctcgca aaaaggcagg ttgagatcgc gaaaaaagtc
     4081 ggtgctgacg ccgtcgccca tggttgcacc ggcaagggca acgatcaggt gcgcttcgaa
     4141 ctggctgcga aagcgctggc acccgaactc accatcattg ccccgtggcg tgaatggaag
     4201 atcaactcgc gagaagaggc gatcgcgtat gcccgcgagc acaatattcc ggtcgaacaa
     4261 accacaaaga acatctacag ccgcgaccgc aatat
//
LOCUS       DATNDT010000308         9596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1010930, whole genome shotgun sequence.
ACCESSION   DATNDT010000308 DATNDT010000000
VERSION     DATNDT010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9596
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1010930"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VN788_09960"
     CDS             complement(<1..283)
                     /locus_tag="VN788_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20893.1"
                     /translation="MRKVALGTLALASVLAAGSAVVGHELVPGFLHPYRAPMSTRRLA
                     RVDTMLERVHATREDFDVRAQDGVVLRGWKIRAAAPNGDWVILLHGQGDN"
     gene            complement(434..901)
                     /locus_tag="VN788_09965"
     CDS             complement(434..901)
                     /locus_tag="VN788_09965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20894.1"
                     /translation="MSISGLQAGLKLLQYRQIVAQCVVFLSVLLFGGIYIYVSLLFGF
                     AYYGIARVEGIPYGWANAFVTSFFIPFQFSDLPHNIYVKILAGIHCIFVVAVGAGTVF
                     GYLQKKMNSLYSVADSLNRRFQEDELRNSVEILTARFGPKKTSTTAPDSQNIK"
     gene            1663..3027
                     /locus_tag="VN788_09970"
     CDS             1663..3027
                     /locus_tag="VN788_09970"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXU20895.1"
                     /translation="MDAEKQIAFRQHLRLCIGELKEIWKPIGIILSISTGLCSVKAFY
                     VGTGAFFLAGVSLLTVAIAVLFTVPITSLRLLARRLIAFVFDLMLFGSITIGLLSPLY
                     DLSLMRPNAATSLGIVWLWFFAFTVLSWRFSGTPGMKIAGLSIRAASGTKTIFLQFLG
                     RGLLTLIVPVLIAGRILSSAAFSTRFSQYVQMSLAVFILVFVPLSVGFSGGQSLPDLL
                     FGLRVLPKRGKALRYPVFLNRKRVLLLFAASLAGGLAYGLISKAELKAYRPMGGQGQT
                     PVAQWLVSTAADQRDAARIRPYVEQGLVIGPDDFVQNVTVLSAYGKLPEPFPRQAPVE
                     CQMGPTAQKGYRLIVVTVEPGVPALVEAKLFSNLLYIESKGSNRPGFLVLELVRRYDY
                     GVFGTNAEDYYTFCLAGTDTSPEDYSPMVSRSIFSYGSISQLGLLLSGEVEGYATLYR
                     LPIF"
     gene            4526..6055
                     /locus_tag="VN788_09975"
     CDS             4526..6055
                     /locus_tag="VN788_09975"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU20896.1"
                     /translation="MALLCAFALAVALAPETRAQDASGAPGGISVQVSEQIFDTLCAL
                     DAAGFNADASTLGDMPERLALRTELLKMQGPATLAVRSYYQGHPVADPSALLSRYITF
                     ALVLGPPPDFTFQVSEDEMPPDALSLRDFQPLLAAFYKEANLGPRWQKVEPEYEVLAG
                     SYRSIVRGTVVKTNAYLREVIGSQHGRSFTVYVDPLAGSSTNFRNLGDQYAVVVGGRP
                     TQSAGLIQHAYLHFMLDPLVLKDRDKLQSKSAILNIAAHAPQLPDEYKTDFVSYVDEC
                     VVKAVELRLRDLSTSEQEKVLQQDDESGFVMVRPLVTGLHAFEKDNPSMTYYFPDLIS
                     GVDVAAEQKRLQGITFASAQPAPAAPTSAQTADAAKQAMLAQGDREIALHDAAAAADI
                     FTKVLDAYPNEPHALYGLAVASVLSGHADESKELFEKVVASSSSGNAANQPDPTVLAW
                     SHIYLGRMDDLEGSRDLAVKEYRAALSVAGAPESARVAAQGGVDHAYSVPKRDGGSGS
                     Q"
     gene            6113..6964
                     /locus_tag="VN788_09980"
     CDS             6113..6964
                     /locus_tag="VN788_09980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20897.1"
                     /translation="MKLSKLSPKIGVIALATSAALFGAAVIQAQGNGQAAPSLKDNGQ
                     GGNSAQGGKNAQGRGPAIDPAEEAGIKAFNAATTDDAKIQAGEDFEQKFPNSRYIEGI
                     QGTLVTLYYNKQDWAKFYPEADKALAKDPNNVPVLTLVGWVIPRVFDPKDPSEPAKLD
                     KAEQDEKHALELISTMQKPASLTDDQFNQAKASAASQAHSGLGMTYFRKNDFQDSSKE
                     LEAATTETSDVDPADLYILGMDYQKLNRNSDAAAAYTKCAAIPGGLQDACKKSASSLS
                     GSTLTPQ"
     gene            6983..7675
                     /gene="rnc"
                     /locus_tag="VN788_09985"
     CDS             6983..7675
                     /gene="rnc"
                     /locus_tag="VN788_09985"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HXU20898.1"
                     /translation="MMVDERETIEALLGYCFKEASRLDEALTHRSLRQGLGDKDNERL
                     EFLGDRVLGLVVSDRLCHLYPAWDAGQLSKGLARLVNADTLQVAARRLNLGEHLRLGP
                     GEEKTGGRDKKRLLSDAYEAVLGAIFLDGGLPPAAEFAERTLLIPAMKAEVEALERSD
                     HKSALQEWLQERGMDIVEYRLTDTAGPDHQKTFEVEVWHAGKQLSLATGRTKKEAEQA
                     AAKLALEVLLAS"
     gene            7693..8532
                     /gene="lepB"
                     /locus_tag="VN788_09990"
     CDS             7693..8532
                     /gene="lepB"
                     /locus_tag="VN788_09990"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXU20899.1"
                     /translation="MEEIEARVPEIPIAENSSAANAESQIKGERVRQVRGSGSLVELC
                     QTLLITVLVALFGTTFVVQAYKIPSDSMSPTLLAGDHLLVNKFIFGGNGAWYEKLLPY
                     REIRRGDLIVFKYKYEDHPDYVKRVIGMPGDRLRIVLGRVFINGKLLREPYAVHEGFN
                     SDAGTDTFPPLDPYSTRVGLLPEWADQIENYIEDDELVVPADRYFVMGDNRDDSQDSR
                     YWGFVDRSAVTGEPVLIYWSVNASEDDYTDEGFWGRIKGIGDTLVHLRSRTRWHRIFR
                     EVH"
     gene            8537..>9596
                     /locus_tag="VN788_09995"
     CDS             8537..>9596
                     /locus_tag="VN788_09995"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXU20900.1"
                     /translation="MELRPSILQGAPTHVIEPPTKASESARPTFLRRLLVWAILLALL
                     MVIGDGIGLLVVSRQRVRQRLNARLEAAFGRPVEVDRYSFSLWGGPTLEADGIRVGED
                     PRFGNEYFLRADSLAVRLRWLSLLRGHIGIGALSLSGPTVNVVNDSAGDWNLAEWLGH
                     PAASAGNSVGPEAIPFAPSFREIEVDGGRIDFKNGDEKLPFAFIGVNGAIYADGPQRW
                     RLDLEAAPWRAAEILQQPGKIHVAGSVGGTSSALRPASVEISWTDASASDFLRLMTGD
                     DSGIRGTVAIGLNAQTSAEGWAIQGQALLGQLHRWDLTLRPDLPAVDVPSLNVTAKML
                     LDVPASTLQITDLSVQAPR"
BASE COUNT         2143 a         2495 c         2927 g         2031 t
ORIGIN      
        1 gattgtcccc ttgaccgtgc aacaggatca cccaatcgcc attcggcgct gccgcgcgaa
       61 ttttccatcc acgcagcaca actccgtctt gagcgcgcac gtcgaagtct tcgcgtgtgg
      121 catgcacgcg ctcgagcatt gtatccacgc gcgccagcct tcgcgtggac attggtgccc
      181 gataaggatg caggaaacca ggcacaagct catgcccgac taccgccgat cccgccgcca
      241 gtactgaagc cagcgccaaa gtgccgagcg caacttttcg catgttcaat aactattacg
      301 ctgattgggt cgatttagtt cgttaaaagg gcaaattccg ccacccgggg gcgtaggtcg
      361 aggcttagcg gtgatcgcgc gtggcgcaca ggagtgctct ttcgatcggg cagccaaagg
      421 ccagatgcga tgtttacttg atgttctgac tatccggagc ggtcgtcgag gttttctttg
      481 gaccaaacct cgcggtaaga atctcaacac tgtttcgaag ttcatcctct tggaaacgtc
      541 ggttcaggga atctgccacg gaatatagag aattcatttt tttttgcaga tacccgaaaa
      601 ccgtcccagc ccccacggcg acaacgaaga tgcaatgtat accagccaga attttgacgt
      661 agatgttgtg cggaaggtcc gaaaactgaa acggaataaa gaacgacgtg acgaacgcgt
      721 tggcccaacc gtagggtatg ccttcgacac gggcaatgcc gtagtacgcg aagccaaaga
      781 ggagtgaaac gtatatataa atgccaccga acaaaaggac gctaagaaac acgacgcatt
      841 gagcgactat ctgccgatat tgcaatagct ttaatcctgc ttggagcccg ctgattgaca
      901 tccaaacctg ccggaggtct tgggtgtcct cggcctggct actgcacgcg atcgccttat
      961 tgataagttt ctccgcccaa ctctttatgc gtccttctaa ttggtataac cctcgcaagg
     1021 aaaaaacggc caatgctgac actttcgcga gggtcttccc taaatgtacc aaaacgatga
     1081 cgagtgccgt gtacagaagc caactatggg aacttagaag cagcagtagt gcccaaagaa
     1141 gagtgaattg aaagaaaggc cgcaatatat tgcggcggac gcgattccaa attcgtcttt
     1201 tcacagacgg cacagcgagg gcagtcgttc ccggatttaa ttttcgaacg ttacgagcaa
     1261 ctccgtcggt ttttggtgct ttccctcgcg aaataaacag tcgcccggcg cggtacgcta
     1321 agcgggttgc tagaagcaaa aggacaaatg gaaaaaagta aatgtaaagt gcgtcgatga
     1381 tcagattttt gacaccgtag ctcatgagaa cggagaggct gatgatcagg accagaactg
     1441 tcgtttcccc aaggttcagg tgcaggcgcg cagctatcgg agacagccgt ggcggggcca
     1501 atcgcagtaa caggagtgca ttgagccata atgcggttgc aaggacgaag cgtgctagtc
     1561 gacgaatcca ttggaggacg gtcgaatgct tagttggcat tagcgatgga ctctacttca
     1621 ctcccaagta ataggcaagc ctgctatact ttcgcgaaat tcatggacgc agaaaaacaa
     1681 atcgcttttc gccagcacct gaggttgtgc atcggtgagc ttaaggagat atggaagcca
     1741 atcggaataa tcttgtcgat ctccactgga ttatgttcgg tcaaggcatt ttatgtaggt
     1801 actggcgcat tttttttggc tggagtatcg ttgcttacgg tcgccatagc cgtattgttt
     1861 acggtgccga ttacttctct tcgactcctc gcaaggcgcc tgatagcttt tgtcttcgac
     1921 ctgatgctat ttggttccat tactataggg ttgttatcac cgttatatga tttgtctctc
     1981 atgcgaccca atgcagccac atcgttaggc atagtgtggc tttggttttt cgcttttacc
     2041 gtccttagtt ggcgcttttc aggaacgccg ggcatgaaaa tcgccggtct gagcattagg
     2101 gccgccagcg gtacgaagac gatttttctc caattcttag ggcgtgggct cttgaccctg
     2161 attgttccag tactgattgc tggacgcatc ttaagttccg cggccttttc aacgaggttc
     2221 tctcaatatg tacagatgtc gctggctgtc tttatcctcg tttttgtacc gctctcagta
     2281 ggcttctcag gcggacaatc gctgcccgac ctcttatttg gattgagagt attacccaag
     2341 cgcggcaagg cgcttcgata tccggtgttt ctaaaccgga agcgtgtcct gttgcttttt
     2401 gctgcatcat tggctggtgg gttggcatat ggccttatct cgaaggctga gttaaaggcg
     2461 tataggccga tgggtggtca gggtcaaaca ccggtagctc agtggctggt atctacggcg
     2521 gcggaccaac gggatgctgc gcgaatacgc ccctatgtcg agcaagggct cgttataggg
     2581 ccagacgact tcgttcaaaa tgttacagta ttatcagcct acggcaaact tccagagcct
     2641 ttcccgaggc aagcgccggt cgaatgccag atggggccga cggcgcaaaa aggttaccgg
     2701 cttattgttg tcacagtcga accgggtgta cccgcccttg tcgaggcaaa gttgttcagc
     2761 aacctcctat atatcgagag caagggaagc aataggcccg gctttctggt gctcgaattg
     2821 gtgcgccgtt acgattatgg tgtgttcggc acaaatgcag aggattacta cacattttgt
     2881 ttagcgggca cagatacgtc gccggaagat tattcgccaa tggtttcgcg atcaattttc
     2941 tcgtacgggt cgatcagcca gttgggtctt ttgttgtcgg gtgaagtcga gggctacgct
     3001 acgttgtatc ggctgccgat cttttaaacg tggcgttgct gtggcgcctt aactgaagac
     3061 tagaccgcag gcgtccgcac ccgcgtaatt ggcaagcgat gcggaatttg catcagcagg
     3121 tattccacgt gaccgcaatg aagcagtaac ggcctgcatt gacatactgc gatttggtac
     3181 acagcgccac gtggtattca tcgagcccaa gagcgctatg cgtcggtgtc gcacctacgg
     3241 tgctcggagg ctcatttgat tccgtccccg cccttccgga gcggggctaa gttgtgtcgc
     3301 gcctacggcg cttggggctt ctcttcgata acgcaagaat gccactcggg agaatggcgt
     3361 tcccggggag agtcgaaagg caacggcgaa agataacggc tggccccagt atttcggggc
     3421 gggcgctgcg gagtaaaact gaatcaaccg caaaacgccc accgagaaac gcgcgcaacc
     3481 cctcgccaag ttactcgggg caagaattcg cgcgcaccct tcgcgtgctc agggcgggac
     3541 ggtggtcgat acgaaacaca atttgactgc gaattcaaaa gcgcccgcct aaaccatatg
     3601 gcggccgcta cgtaagcaaa ggcaaatgca aaaaataaga gaggtccttc gccttgccgc
     3661 tgaaaacgcg gcaaggctca ggatgacgag cgcattttat tttttgcggt cagcattgtt
     3721 gagaaatcat gaattgtggt gcgagctcca aaacgcccac cgagaaacgc gcgcaacccc
     3781 tcgccagttg ctcggggcaa gaattcgcgc gcacccttcg cgtgctcagg gcaagacggt
     3841 ggtcgatatg aaatacaatt tgactgcaaa ttcaaaagcg cccgcctaaa ccatatggcg
     3901 gcctctacaa acggcaaagg cgacggcacg ggcaaaagcg cccgaccgga tggcgggtcg
     3961 gcggcgataa aagcaagggc gggaaggcaa acctgcgcgg ccagccgtgt gccacgaaat
     4021 gcaaatgcga cggaaatgca agccaccaca gccaggagtg gctgtgccac gcaaggcggt
     4081 cggtcgctac aaattcaagt gcggaggcag tggaacggcg cccgcgcaaa aggcggacat
     4141 tgttgcccag agtttttggg aagcgggaga gagtggctag tatctagtgg cgagtgacta
     4201 gcgtgcgtat ttcggtggat tctgacacga aaccggcggc aaaggtgccc gaccaaacag
     4261 cgggtcggtc gctgcaaaat ctaaagcgca cggccggaaa agcccggcgt tctagaattc
     4321 aaggtcccag agctcccgca gacgcgacaa cgcgcattct tgactaaccg ggtgctgctc
     4381 gatagagtgg gcgcagtgga agattttgcg acggttggca gcgtcgcgac tagaagctgc
     4441 tggacgatcc agttagaagc tactggacga atcagggaag gtacccatcc gaaaaatgct
     4501 ccatggattg gtgtggcgcg gtgcaatggc gctcctttgc gcattcgcgc tggctgttgc
     4561 gttggcgccg gaaacgcgcg cgcaggatgc gtcgggcgca ccgggcggga tcagcgttca
     4621 ggtcagcgag caaattttcg atacgctgtg cgcgctggac gcagcgggct tcaatgcgga
     4681 tgcgagtacc ctcggcgaca tgccggaacg gctggctttg cggacggaac tgctgaagat
     4741 gcaaggtccg gcgactctcg ccgttcgctc ctattatcag ggccatcctg tcgccgatcc
     4801 gagcgctctg ctttcgcgct acatcacatt tgctctcgtg ctcggccccc ctcccgactt
     4861 tacgtttcaa gtaagcgaag acgaaatgcc gccggacgct ctttccttgc gggattttca
     4921 gccgctgctc gctgcgttct ataaggaagc gaatcttggg ccgcgctggc agaaggttga
     4981 gccggaatac gaggttctgg caggcagcta tcgctcgatc gtgcgcggga cggtggtgaa
     5041 gacgaacgct tacctccgcg aagtgatcgg cagccagcac ggccgctcgt tcacggtgta
     5101 cgtggatccg ctggcgggaa gcagtacaaa tttccgaaac ctgggcgatc agtatgccgt
     5161 cgtagtaggg gggcggccaa cccagtcagc cggtctgatt cagcacgcgt atttgcactt
     5221 catgctggat cctctggtgt tgaaggaccg ggataaattg caatcgaaaa gcgcaattct
     5281 gaacatcgcg gcgcacgctc cgcaacttcc cgacgaatat aagacggatt ttgtgtcgta
     5341 tgtcgatgag tgtgtggtca aggcagtcga actgaggttg cgcgatcttt cgacttccga
     5401 gcaggaaaaa gttttgcagc aggacgacga gagcggcttc gtgatggtgc ggccgctagt
     5461 gacgggcttg cacgcattcg agaaagacaa cccgtcgatg acatattatt tcccggacct
     5521 aatctccggc gtcgatgtgg ccgccgaaca gaagcggctg cagggcataa cgtttgcgtc
     5581 ggcgcagccc gctccggcgg cccctacgtc cgcgcaaacg gcagatgcgg cgaaacaagc
     5641 gatgctggcg cagggagatc gtgaaattgc gctgcacgac gcggctgcgg cagcggacat
     5701 ttttacaaag gttttggacg cgtatccgaa tgagccgcat gcgctttacg ggctggcggt
     5761 ggcctcagtc ctttcgggac atgcggacga aagcaaggaa ctgttcgaaa aggtcgtggc
     5821 aagctcatcg tcggggaacg cggcgaacca gcctgatcct acggttctgg cgtggtcgca
     5881 tatttacctg ggacgcatgg acgatctgga aggaagccgc gatcttgccg tgaaggagta
     5941 ccgggccgct ctgtcagtgg ccggggcgcc ggaatcggca cgcgtggcgg cacaaggagg
     6001 cgtcgaccac gcttattcgg tgcccaagag ggacggggga agcggttcgc agtagccggg
     6061 aagcaatagt gctcgattcg agccatcttt agcgacgtat aaaggattcc atatgaaact
     6121 cagtaagcta agtccgaaaa tcggcgtgat cgcattggcg acatccgcag cgctctttgg
     6181 cgctgccgta attcaggcgc agggcaatgg gcaagcggcc ccttctctta aggacaacgg
     6241 ccagggaggg aacagcgctc aaggcggaaa aaatgcgcag ggacgcggac cggcgatcga
     6301 tccggccgaa gaggcaggca ttaaggcgtt caatgccgcg acgaccgacg atgcgaagat
     6361 tcaggcgggc gaggatttcg agcagaagtt cccgaacagc cgctacatcg agggaatcca
     6421 gggaacgctc gtgacgctgt attacaacaa gcaggattgg gcgaagttct atccggaggc
     6481 cgacaaggcg ctcgcgaagg acccgaacaa tgtgccggtg ctcacgcttg tcggctgggt
     6541 aatcccgcgt gtgttcgatc caaaagatcc cagcgagcca gcgaagctcg ataaagccga
     6601 gcaggacgag aaacatgctc tcgaactgat ttcgaccatg cagaagccag cgtcgctcac
     6661 ggacgaccag ttcaatcagg caaaagccag cgcagcttcg caagcgcaca gcggactcgg
     6721 catgacgtat ttccgcaaga acgatttcca ggattcttca aaggagcttg aggccgcgac
     6781 aaccgaaaca tcggatgtcg atccggccga tctgtacatc ctgggaatgg attaccagaa
     6841 gctgaatcgc aacagcgatg cggcggcagc gtacacaaag tgcgcagcca ttcccggcgg
     6901 tttgcaggac gcgtgcaaga agtccgcgtc gtctttgtcg ggatcgactc tgacaccaca
     6961 gtaaaacggg cctgccgtgg cgatgatggt ggacgagcgg gaaacaatcg aggcgctcct
     7021 cggttactgt tttaaagaag cgagccgcct cgatgaggcg ctcacacacc gttcgcttcg
     7081 ccagggcttg ggcgacaaag acaacgaaag gctggagttt ctgggcgacc gggtattggg
     7141 gctggtggta agcgaccggc tttgccatct ttatccggcg tgggatgccg ggcagctttc
     7201 caaggggctg gcccggctgg tcaatgccga tacgcttcaa gttgcggcaa gacggcttaa
     7261 cctgggcgag catttgcggc tggggccagg ggaagaaaaa acgggcggcc gcgacaagaa
     7321 acggttgctc tccgatgcat atgaggcggt actcggggct atttttctag atggtggcct
     7381 cccgcccgca gccgagtttg ccgagcgcac gctgctcatt ccagcgatga aggccgaggt
     7441 ggaggcgctt gagcgttcag accacaagtc cgcgcttcaa gagtggctgc aggaacgtgg
     7501 catggacata gtggagtacc ggctgactga caccgccgga ccggatcatc agaaaacctt
     7561 cgaagtggaa gtctggcacg ccggaaagca actctcgttg gcaacgggcc gcacgaagaa
     7621 agaagccgaa caggcggctg cgaaattggc gctcgaagtc ctcctggcaa gttaatttcc
     7681 gggaaggacc gcatggaaga gattgaggct cgagtgcccg aaattcccat agcggagaac
     7741 tccagcgcgg cgaatgccga gtcgcagatt aaaggcgagc gagtccggca ggtgcgtggc
     7801 agcggctctc ttgttgagtt gtgccagacg ctgctcatca cagtcttggt cgcgctattt
     7861 ggcaccacgt ttgtggttca ggcatacaaa attccctccg attcaatgag ccccacgctt
     7921 ctcgcggggg atcatctcct ggtgaataaa tttatttttg gcggcaacgg cgcctggtac
     7981 gagaagctcc tgccttatcg cgaaattcgc cgtggcgacc ttatcgtttt caaatacaag
     8041 tatgaagatc atccggacta cgtgaagcgc gtgatcggca tgccgggaga tcggttgcgg
     8101 atcgtgttgg ggcgcgtctt catcaatgga aaactgctgc gggagccgta tgctgtacat
     8161 gagggattca attcggatgc gggcacggac acgttcccgc cgctggatcc ctatagcacg
     8221 agagttgggc tcctgccgga atgggccgat cagatcgaga actatattga ggacgatgaa
     8281 cttgtggttc cggccgaccg gtatttcgta atgggagata atcgggacga cagccaggac
     8341 agccgctact ggggatttgt ggatcgcagc gcggtgactg gagagccggt gctgatctac
     8401 tggtccgtaa acgcttcgga ggatgattat accgacgagg ggttctgggg caggatcaag
     8461 gggatcgggg acactctcgt tcacctgcgc agccgaacac gctggcaccg aatatttcga
     8521 gaggttcact agccaagtgg aactcaggcc gagcatcctg caaggagcgc cgactcacgt
     8581 tattgagccg ccgacgaagg cgagcgagtc tgcgcgccct acgtttctga ggcgattact
     8641 cgtatgggcg atcttgctcg ctttgctgat ggtgatcggc gacggcatcg gattgcttgt
     8701 tgtgagccgc cagcgcgtgc ggcagcggtt gaacgcgcga ctcgaagcgg cttttgggcg
     8761 acctgtggaa gtggatcgct actcgttcag cttatggggc gggccaacgc tcgaagccga
     8821 tggaattcgc gtcggggaag acccgcgctt cgggaatgaa tattttttgc gcgcggactc
     8881 gctggcggtg cggctccgtt ggttgagttt attgcgcggg cacatcggaa ttggggcgct
     8941 ttcccttagc gggccgacgg taaatgttgt gaatgattcg gcgggggact ggaatctggc
     9001 ggagtggctg ggccatcctg ccgcgtccgc cggcaatagc gttggaccgg aggcgattcc
     9061 gtttgcgccg tcgtttcgcg agattgaagt ggacggtggg cgaatcgact tcaaaaacgg
     9121 cgatgagaag ctgcctttcg cgtttattgg tgttaacggt gcgatttacg cggatgggcc
     9181 gcagcgctgg cgccttgacc tagaggccgc cccgtggcgc gccgcggaaa tcctgcaaca
     9241 gccgggtaag atccacgtgg cggggagcgt cggagggacg tcatcggcgt tgcgtccggc
     9301 gagcgtggag atcagttgga cggacgcttc cgcatccgac tttctgcgac tgatgacggg
     9361 cgacgattcc ggtattcgcg gaactgttgc tatcgggctg aacgcgcaga ccagcgcgga
     9421 aggatgggcg attcaagggc aggcattgct cgggcaattg catcgttggg acctgacgct
     9481 gcggcccgac ctacctgcag tggatgtgcc ttcgctgaat gtgacggcga aaatgctgct
     9541 tgatgtgccg gcttcaacgc ttcaaatcac cgatttatcc gtgcaggctc cgcgct
//
LOCUS       DATNDT010000309         4614 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1841746, whole genome shotgun sequence.
ACCESSION   DATNDT010000309 DATNDT010000000
VERSION     DATNDT010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4614
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1841746"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="VN788_10000"
     CDS             <1..606
                     /locus_tag="VN788_10000"
                     /inference="COORDINATES: protein motif:HMM:NF013156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HXU20901.1"
                     /translation="SITDANMQTIASGTDTNARVYRSDEQPLPSSTNVNASNQQQPAP
                     VVPQAAVSSTPQPNTPGAPTPGAAAQLHFDPANTTMKVGDSTVIGVAVSGVNDLYSIP
                     LMIHYNPAVLSVQEVRDGGFLSQPDVILQHVDEKQGEVVVNAARGKAPGVNGAGTLIG
                     LVIRAIAPGTSSLQVLQVNAKNSQQQSIPVAPFEATIQVQQ"
     gene            702..1205
                     /locus_tag="VN788_10005"
     CDS             702..1205
                     /locus_tag="VN788_10005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20902.1"
                     /translation="MTLLELMVACSILIILATVAMPMERVVVRRHKEAQLRYDLQQMR
                     DAIDRYKDAADKNLFQVEADTNGYPPDLETLVDGVELTGTGSPTGVSNALSGLSSNTN
                     AAESKSRKLHFLREIPVDPMTGEKDWVLRSVQDDPDSTAWGGQDVFDVHSSSTDTALD
                     GTKYSDW"
     gene            1205..1663
                     /locus_tag="VN788_10010"
     CDS             1205..1663
                     /locus_tag="VN788_10010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXU20903.1"
                     /translation="MNFMVHIPGPNRAPRAGLAPRAAASGDGGFTLLEIMVVLAIFVI
                     LMTLAAGSYQRSVLHAKEAALHQDLAEMRKAIDDYTMDKEAAPNTLDDLVQAQYLHEI
                     PKDPLTGKREWNTDTCDELLSPDQTSTGICNVHSESDGVSPLENSAYSSW"
     gene            1921..1997
                     /locus_tag="VN788_10015"
     tRNA            1921..1997
                     /locus_tag="VN788_10015"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1955..1957,aa:Met,seq:cat)
     gene            complement(2009..3163)
                     /locus_tag="VN788_10020"
     CDS             complement(2009..3163)
                     /locus_tag="VN788_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXU20904.1"
                     /translation="MLRWREDVIENGVVRRIYRSEVLGDKSEYPTRRLALRAADDRLS
                     VVNSPNYRARPTATFRQFAARWQSTVLPQHKPSTQATVRSHIGKYLVPFLGDRCLKDV
                     NAELIQVFISGIKAAPKTVRNIYVTLQIMWKSARAWGYVAHDCVSDVVLPKPRRSERF
                     FFTLEEAQRILTTGDFCVWPRSRSRANVIKAEPYRTFYWIAVETGLRAGELCGLRVDD
                     LDLERCLLRVEQSSWRGKLQLPKTENAVRSLAISPELALHVRDFIARWRPNRQRLIFA
                     SRAGTPWDANLLVKRKLQPTLRSLGIRLCGLHAFRHANETLMDRLGVPLKVRQQRLGH
                     SDPRLTLGVYTHVASEDDVKIAGQLGRILHPFGPTDKIKGLADFRQTPLN"
     gene            3377..3697
                     /locus_tag="VN788_10025"
     CDS             3377..3697
                     /locus_tag="VN788_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20905.1"
                     /translation="MDDANARKLEETAITTLDHVRNKNFRKEIVKLDGKKFSNCSFDR
                     CILEYSGGDTEIGFRCSTTGIRPRFKGAAARTVRLLHALGLLSFDPFAESDEWPKQNA
                     TAAD"
BASE COUNT         1013 a         1279 c         1282 g         1040 t
ORIGIN      
        1 tcgatcaccg atgcaaatat gcaaacaatt gcgtcgggaa cggacacgaa cgcgcgcgtc
       61 tatcgctctg acgagcaacc gctgccatct agcacaaacg taaacgcatc caaccagcag
      121 cagcctgcac cagtagtgcc ccaagccgct gtctccagca cgccgcagcc gaatacgccc
      181 ggtgcgccaa cgcccggcgc cgcggcgcag cttcatttcg atccggcgaa tacgacgatg
      241 aaggtaggtg attcaaccgt gatcggcgtc gcggtttccg gcgtgaacga tctctattcg
      301 atcccgctga tgattcatta caacccggct gtccttagcg tgcaggaagt ccgcgatggg
      361 ggtttcctct cgcaaccgga cgtgatcctt caacacgtgg acgaaaagca aggagaagtg
      421 gtggtgaacg cggcgcgtgg caaggctccg ggagtgaacg gcgcagggac gctgatcggg
      481 cttgtgattc gcgcgatcgc tcctgggaca agctcgctgc aagtgttgca ggtaaatgcg
      541 aaaaactccc agcagcaatc gattccggtt gccccgtttg aggcaacgat tcaggttcag
      601 caatagccag tgaagacatg caggcggcgc gcaaattcgc attgacttac ggggcaggga
      661 agtcgcctgc gcgagcgcgc cgtttacaag gctgcgcggg catgactctg ctggaattga
      721 tggtcgcgtg ctcgatcctt attattcttg ccaccgtagc aatgccgatg gagcgcgttg
      781 tggtgcggcg gcacaaagag gcgcaactgc gctacgactt gcagcagatg cgcgatgcga
      841 tcgatcgcta caaagacgcc gccgacaaga atcttttcca ggtggaagcc gacacgaacg
      901 gatatccgcc cgaccttgag acgctggtcg atggagtgga gcttacagga acaggcagcc
      961 cgaccggcgt gtcaaacgcg ctgagcggat tatcttcgaa tacgaatgcg gccgaatcca
     1021 aaagccgcaa gctgcacttt ctgcgcgaaa ttccagtcga tccaatgacg ggagaaaagg
     1081 attgggtact gcgatcggtg caggacgatc ccgattccac ggcgtggggc gggcaggacg
     1141 tcttcgatgt ccacagcagc tcgaccgaca cggcgcttga cggaacaaag tattcggatt
     1201 ggtgatgaat ttcatggttc acattccggg accgaatcgc gcgcctcgcg cggggttggc
     1261 gcctcgcgcc gccgcaagtg gagacggcgg ctttacgctg ctggaaatta tggtcgtgct
     1321 ggcaatcttc gtcattttga tgacgctcgc cgctggcagc tatcagcgct ctgtgcttca
     1381 cgcaaaggaa gccgcgctgc atcaggattt ggcggagatg cgcaaggcca tcgacgatta
     1441 cacgatggac aaagaagcgg cgccgaacac gctggatgac ctcgttcagg cgcaatattt
     1501 gcacgagatc ccgaaagatc cgctcacggg aaagcgcgag tggaatacgg atacctgcga
     1561 cgaattactg agtcccgatc agacctcgac gggtatatgc aacgtgcact ccgaatcgga
     1621 cggcgtctcg ccgcttgaaa actccgccta cagctcgtgg tgacattccc gttgaccttt
     1681 aggattcgtt cgcgccgctc tgacgatttc tttcctcgaa caatcgtttg accgatagtg
     1741 ccgcatccgc tatccgcgga gcagcctggc agctcgtcgg gctcataacg cgccaaacac
     1801 caccttgccc tgcacgtgcg ttttctgctc gcgggtcgtc cgtcgttttt ttttcggttt
     1861 ttcccggcca cagaggttga gagccagagg aaaaacctaa gttatgtgtt atgatgcttc
     1921 tgcggggtgg agcagcctgg tagctcgtcg ggctcataac ccggaggtcg ttggttcaaa
     1981 tccaaccccc gcaaccatgc catcttattc agttcaaagg ggtttgccga aagtcggcaa
     2041 gcccttttat tttgtctgtg ggaccaaatg ggtgcagaat tcgcccaagt tgccccgcga
     2101 ttttgacatc gtcctcactt gcaacatgcg tgtacacccc gagagttagg cgcggatcgc
     2161 tgtgaccgag cctttgttgc cgaaccttca atggcacgcc gagacggtcc atgagcgtct
     2221 catttgcgtg tcgaaacgcg tgaagtccac acagccgaat ccccaatgac cgaagagtcg
     2281 gctggagttt tcttttcacg agaagattgg catcccacgg cgtacctgcg cgagatgcaa
     2341 agatcagtct ttgtcggttt ggcctccaac gcgcgataaa gtctctgaca tgcaaagcca
     2401 attctggaga aatcgccagc gaacgcacgg cgttctccgt cttcggcaat tgcaattttc
     2461 ccctccaaga actttgctcg acgcgaagca gacagcgttc caggtccaaa tcatccacgc
     2521 gaagcccgca caattcgccc gcccgtaagc ccgtctcaac ggcaatccaa taaaacgttc
     2581 tgtacggttc ggctttgatg acgttagccc tgctgcgact acggggccaa acgcaaaaat
     2641 cgcccgtcgt caaaatccgc tgcgcttctt ctagggtgaa aaagaaacgt tcggatcgtc
     2701 gcggcttagg taaaaccaca tcggacacgc aatcgtgtgc gacgtaaccc caagcccgcg
     2761 cggacttcca catgatctgt agcgtgacgt agatgtttcg aacggttttc ggcgcggcct
     2821 tgattcccga aataaaaacc tgaatcaact ccgcgtttac atccttcagg caacggtcac
     2881 ccaaaaacgg aacaaggtac tttccaatat gcgagcgaac ggtggcctgg gttgatggtt
     2941 tgtgttgcgg gagcactgta gattgccagc gtgcggcaaa ctgtcgaaag gttgccgttg
     3001 gacgtgcgcg gtaattcgga ctgtttacga cggaaagacg atcatcagcc gcgcgaagcg
     3061 ctaaacgtct cgttggatac tcggacttgt cgccgagaac ctcagagcga taaattcgcc
     3121 gcactacgcc gttttcgatc acgtcttctc gccaccgcaa cacccaacga ctgcccctga
     3181 gaaacagact gcccttttga aaacgtcggc gagccaatac aggcccttcc tgctcgccga
     3241 tctgttgccc tcggttgagc atggtagacg ggcagtctgt ttctggcaat aaacttgttt
     3301 cgacgtttgg catttgctcg tgtcttgacg gtctgtcgag aagggttcac aatctcgcgc
     3361 gattggagtt cttcacatgg atgatgcgaa cgcacgaaaa ttggaagaga ctgccattac
     3421 gactttggat cacgtcagaa ataaaaattt caggaaagaa attgtcaaac tggacggaaa
     3481 gaaattttca aactgctcgt ttgatcgctg cattcttgaa tattcgggcg gcgataccga
     3541 gataggattc cgctgctcaa cgaccggcat ccgcccgcga tttaagggcg cggctgctcg
     3601 cactgttcgc ttgctgcacg ctctcggctt gctgtccttt gatccgttcg ccgaaagtga
     3661 tgagtggccc aagcaaaacg ctactgccgc cgattaagat gcgcggcatt tgtcgccgct
     3721 tgctatcgcg ctgctttttc attatttcca ccgcacaact cgcgcccgca atccacgaat
     3781 acagcatgac gcgccttcct gttccttcgg acgtgctgat ttctgagatt tagtgcagag
     3841 cttgctggcc gtttcaacgg tggcaaccga aaacacctga acggatcgcc gagtggttag
     3901 ggcgctgccg gcatgtcgct gcctcaagct catatttcat tctccgtgtg gcaaaatccc
     3961 tagttatgga ggataggcaa gccgcgtctt ttcaatgtcg gtttgcggcc aagttctatg
     4021 tggcaaaaag agttgttttt ggcccgtgcg agttctaaaa accgacgatc cttgcggggg
     4081 ctcgttgccg tacgtgaaga cgaagtagcg atgcgcgtat cttcttggcg tcttcgagat
     4141 tgtgttcgtt cgtcaaacgc tcaacacacg tgcggcacgt aaagcgggtt gcggcggtcg
     4201 cccctggcgg tgccgggatt ctatgttttt tgcattctgc gcaaacaagc gtgatctctg
     4261 gattcgcagt gacgcgttgc attggcctcc taaaaacaaa tgggcggcgc accccacgtg
     4321 cgggtaaggc acgtcgccca tttcggccac gacgcaaatc tcacccactg cttgcagtca
     4381 gcaggtgcta atttacgcgg gcgcttttgg ccgttcgggt gagaggcccg aaacgtgcca
     4441 aactcaatgc actatgcagt tcggaattat tcggcaggaa ttggtttggg ttgtgggatt
     4501 cgtttaattg tttcggtttc accgtgttcg acgagccatt tcatattcgt cggccgctgc
     4561 gcttcgtcga gaagccattg tgcacgcgtt ttaagttgct cgacgccaat tagc
//
LOCUS       DATNDT010000310         4526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3180275, whole genome shotgun sequence.
ACCESSION   DATNDT010000310 DATNDT010000000
VERSION     DATNDT010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3180275"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VN788_10030"
     CDS             <1..418
                     /locus_tag="VN788_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20906.1"
                     /translation="GASQSIYPTHHLMRDYMSSFCASGSAATIVMDPTVAGLLKDTHG
                     FKTKAQLSEWFSQNVQKTAGSYWGNGVIQSTAVPFALQGLEPYASWRKLPDDALIKPF
                     TNPKAIHVVVAGGKVQTMWFVTDFGIRRGVLIDDWA"
     gene            493..1032
                     /locus_tag="VN788_10035"
     CDS             493..1032
                     /locus_tag="VN788_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HXU20907.1"
                     /translation="MKPQLWAGFFLGSLILMAAVSSRALPNNSSQESSAAKTNQHPST
                     SAKDKIPLPQVPRITAEEVQRLIKEKGNVVLVDTDDSESYGDEHIKGAVNIAYDPTAD
                     PRAQDETLSALPGNKLIVFYCNCPHEEDSAPLVLEMWELNYDHDKVKALQGGLSRWEQ
                     LKYPLAGTSISTASTEKGN"
     gene            1180..3915
                     /locus_tag="VN788_10040"
     CDS             1180..3915
                     /locus_tag="VN788_10040"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HXU20908.1"
                     /translation="MRQHRLALALAGAVVILLIIVPLFSVIIGLLVDRLWFSHIGFRG
                     IFVTILGTQVVMGICCGAVFLLLCGLNFWAARSVALRSGYRRVITGMIEIPAIEKFPL
                     FFWRLMWFGLVLMTWIVGQWAAGHWETYLLATHAVPIAQTDPIFGISLSFYLFRLPFL
                     FFLYHLALIILVISLLTAALEYFITGGVWISPRGLGIGRNACRHLMVLGGTFFLLFAW
                     RARLGMYDLLYAPSGLVYGAGYTDVHVGWPVLWIQLVLCAITAIVFFAGAPKGRMRAA
                     GYAVGGVVGVAILGGLIAPLLVQRYLVAPSELEKEQPYIARNIQFTRQAYGLDRFDER
                     DFPAIQDLTAASVQQNTATMRNLRLWDHDPQLTTFQQLQEIRTYYDFANVYYDRYNIN
                     SELRQVSLSARELSAESLPDPNWVNQHLTYTHGYGLCLGPVNESTSDGLPVLFIKDIP
                     PASSISLKVTRPEIYYGQLSNNYCLVKTSAKEFDHPYGEDNVYKTYAGSGGVPVAGLW
                     RRLIFALRFGDINILISGYIQPTTRIMMYRRVLERVETLAPFLSFDSHPYLIVANDGS
                     LQWIVDAYTTSDQYPYSGPTQDRGNYIRNSVKITVSAYDGSVKFYVSDPSDPLIQAYQ
                     HIFPGVFQPLSEMPSDLRAHIRYPQDFFAIQAAKYAVYHMTDPSVFYSKEDLWRVATR
                     SVNGTSTPMSPYYAVMKLPEVGKSEEFILMVPFTPARKNNMIAWIAARCDGADYGKVL
                     VFTFPKEKLIYGPEQIQSRVNQSPSISQQLTLWNQGGSRVIRGTLLVVPVHNAVLYVE
                     PLYLAAEGGSSIPELKRVIVAYSDKVVMEPSLDDALNAIFGGNRSETAEAAGSVPTTG
                     SAAGRIPGPVENLIRQANQHFEAAQQDLRQGNWSGYGQEMQKLGEILKQMPAKQQ"
     gene            3988..>4526
                     /gene="rpe"
                     /locus_tag="VN788_10045"
     CDS             3988..>4526
                     /gene="rpe"
                     /locus_tag="VN788_10045"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009225258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HXU20909.1"
                     /translation="MSYARIEIAPSILASNFAKLGDEIRAVEAGGADVIHVDVMDGHF
                     VPNISIGIPVVSSLRKATQLPLDVHLMIERPEKYIDDFVKAGANRVLVHEEATVHLDR
                     ALAMIRELGAEAGAAINPATPVAMLAEVLDKVDTVLVMSVNPGFGGQQFIQGAFEKIR
                     QLNQWRARYNLGFRIEVDGG"
BASE COUNT          957 a         1235 c         1290 g         1044 t
ORIGIN      
        1 gggagccagc caaagtattt atccgaccca ccacttgatg cgcgactaca tgagttcgtt
       61 ttgcgcatcg ggatcagcag ccaccattgt gatggatcca accgtggcgg ggttgcttaa
      121 agacacgcac ggcttcaaga ctaaggccca attgagcgag tggttttcgc aaaacgttca
      181 gaaaaccgcc ggctcgtatt ggggcaacgg cgtaatccag agcaccgcag ttccgttcgc
      241 tctccagggt ctcgaacctt acgcgagctg gagaaagctg ccggacgatg ctctcatcaa
      301 acccttcacc aaccccaagg ccattcacgt tgtcgtggcc ggaggaaaag tccagaccat
      361 gtggttcgtc actgattttg gcatacgccg aggcgtgttg atcgacgatt gggcgtagag
      421 ttaaaattaa gttgacgagc ggcgagcgcg gggcaaaaac ccgagctcgt attcaccgga
      481 agggagtgaa ttatgaagcc tcaactctgg gcgggcttct tcttgggatc cctgatactc
      541 atggcggcgg tttcatcgcg cgctttgcca aataattcct cccaggaatc gtccgcggcc
      601 aaaacgaacc aacacccttc gacatccgca aaggataaaa ttcctcttcc gcaagtgccg
      661 cgcatcaccg ccgaagaggt acagcgcctg atcaaggaaa agggaaatgt agtgcttgtg
      721 gacaccgacg attccgagag ctacggcgac gaacacatca agggcgccgt caacattgcc
      781 tacgatccca cggccgatcc gcgagcacag gacgagacgc tctccgcgct tcccggaaac
      841 aagttgatcg tcttctactg caattgcccc cacgaggaag atagcgctcc gctggtgctg
      901 gagatgtggg agctaaacta cgaccacgac aaggtaaagg ccttgcaagg tggtctcagt
      961 aggtgggaac aactgaaata tcctctcgct ggtacctcca tcagcacggc atccacagaa
     1021 aagggtaact gaaaggcatc ccctcacgcg cgcaatgccg agaccctttc gccggcgcgg
     1081 aacgtaatcg tacaggctgc taaaaagccc tgcggatttt gccgtagaac ttttgacacg
     1141 taacaccgca tgtggtcaga tggccgccgg agaggtgata tgcggcagca tcgtctggct
     1201 ctggcgcttg cgggggctgt tgtaatcctt ctgatcattg ttccactttt ctcggtcatt
     1261 atcgggttgc tggtcgaccg gctgtggttt agtcacatcg gtttccgcgg gatcttcgtc
     1321 acgattctcg ggacgcaggt tgttatgggt atctgctgcg gggcggtgtt tctgcttctc
     1381 tgcggtctga acttttgggc cgctcgaagc gtggcgctgc gctcggggta tcggagggtg
     1441 atcacaggaa tgattgaaat tcccgccatc gagaaatttc ctttattttt ttggcggctg
     1501 atgtggttcg gccttgtact catgacatgg atcgttggtc aatgggcggc ggggcactgg
     1561 gagacctatc ttctcgcgac gcacgcggtg cccatagcac agaccgatcc gatctttggg
     1621 atcagcctga gcttttacct gttccgtctg ccctttcttt tttttcttta ccatcttgcg
     1681 ctgatcatct tggtaatcag cctgctgacg gcggcgctgg aatatttcat cactgggggc
     1741 gtgtggattt cgccgcgcgg cctcggcatc ggccgcaatg cctgccgaca tctgatggtt
     1801 ctgggaggaa cattcttttt actgttcgcg tggcgcgcgc ggctcggcat gtacgatctg
     1861 ttgtatgcgc cgagcggctt ggtgtacggc gccgggtaca cggacgttca tgtgggctgg
     1921 cccgtactct ggattcaact ggtcctttgc gcgatcacgg cgattgtctt ttttgccggg
     1981 gcgccgaagg gccgaatgcg agcggccggg tatgccgttg gcggcgtagt cggcgtggcg
     2041 atcctgggtg ggttgatagc tccgttattg gtgcagcgtt atctcgttgc gcccagcgag
     2101 ctcgagaagg agcagcccta tatcgcgcgc aacatccagt tcacccggca ggcctacggg
     2161 ctcgacagat tcgatgagcg cgactttccg gcaattcagg acttgacggc tgcgtctgtg
     2221 cagcagaaca ccgctacgat gcgaaatctc cggttgtggg atcacgaccc acaactcacc
     2281 acatttcagc agcttcagga gatccgcact tattatgatt tcgcgaatgt ctattacgat
     2341 cgatacaaca ttaattcgga acttcgccaa gtttcgcttt cggcccgaga actctcggct
     2401 gaaagcctgc cggatcccaa ctgggtcaac cagcacctga cttacacgca cggatatggg
     2461 ctgtgcctcg ggccggtgaa cgagtccact tcggatggac tacctgtgct cttcatcaag
     2521 gatattccgc cggcctcgtc catctcactc aaggttaccc gcccggagat ttattacgga
     2581 cagctatcca acaattactg cctcgttaag acgagcgcga aagagtttga ccatccatac
     2641 ggagaagaca acgtttataa aacatatgcc ggttcgggtg gcgtcccggt ggctggttta
     2701 tggcggcgtc tgatttttgc gctgcgcttc ggtgatatca acattctcat ttcgggatat
     2761 attcagccaa caactcgcat catgatgtac cgacgcgttt tggagcgtgt cgaaacgctc
     2821 gctccttttt tgtccttcga cagccatccc tacctgattg ttgcgaatga tggctccttg
     2881 caatggatcg tggatgccta cacgacctcc gaccagtatc cgtattccgg cccgacccag
     2941 gatagaggga attacatccg caactccgtc aagatcaccg tcagcgccta cgacggaagc
     3001 gtgaagtttt atgtaagcga tccatccgat ccactgatcc aggcctatca gcacatcttt
     3061 cccggtgttt ttcaaccgct ctcggagatg ccgtcggacc ttcgggcgca catccgttac
     3121 ccccaggact tttttgctat tcaggcagcc aagtacgccg tgtatcacat gacggacccg
     3181 agcgtcttct atagcaagga agatctgtgg cgcgtggcca cgcggtcggt aaacgggaca
     3241 tccacgccca tgagtcctta ttacgccgtt atgaaattgc ccgaggtggg gaagagcgag
     3301 gaattcattc tgatggttcc gtttacgccg gcgcggaaga acaacatgat cgcctggatc
     3361 gcggcgcgtt gcgacggggc cgattacgga aaggtcctcg ttttcacttt tcctaaagaa
     3421 aaactgatct acggcccgga acagattcaa tcgcgcgtca accaaagtcc ttcgatctct
     3481 cagcagctta cgctctggaa ccagggtgga tcccgggtaa ttcgcggcac attgctggtg
     3541 gtaccggtgc ataatgcggt cctgtatgtt gagccgctgt acctggccgc cgagggaggc
     3601 tcttccatcc ctgaattgaa gcgagttatc gtagcctatt cggacaaggt ggtcatggag
     3661 ccgtcgttgg acgacgctct gaacgctatc tttggtggca atagaagcga gaccgcggaa
     3721 gcggcggggt ctgtgccgac gaccggctcc gccgcgggaa gaatccctgg tcctgtggag
     3781 aatctgatcc gacaggctaa ccagcatttc gaagcggctc agcaggacct ccgccaaggc
     3841 aattggagcg gctacgggca ggaaatgcag aagcttggcg agattttgaa acagatgccg
     3901 gcgaaacagc aatagtcgct atgggcggag gccgatttca cgttgcggcg agccgcttct
     3961 cgctgtgcta agatttaccc caacagcatg agctacgcaa gaatagagat cgcgccctcg
     4021 attttggcgt cgaatttcgc caaattgggc gatgaaattc gcgctgttga ggccggcggc
     4081 gccgacgtga tacacgtgga cgtgatggac gggcattttg tgcccaatat ttcgatcgga
     4141 attccggtcg tatcatcgct tcgcaaggca acccagcttc cgctagacgt gcatctgatg
     4201 attgagcggc ccgagaagta catcgacgat ttcgtgaaag cgggggccaa tcgggtgttg
     4261 gtgcatgaag aagcgaccgt ccatttggac cgggcgctgg cgatgattcg cgaacttggc
     4321 gccgaagcgg gcgcggccat caaccccgcc acgcccgtcg cgatgctcgc ggaagttctc
     4381 gacaaagtgg acactgttct agtgatgtcc gtaaaccctg gcttcggagg tcagcaattt
     4441 attcagggag ccttcgaaaa aattcggcag cttaaccagt ggcgcgcacg ctataatctc
     4501 ggctttagga ttgaagtcga tggcgg
//
LOCUS       DATNDT010000311         3224 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1188887, whole genome shotgun sequence.
ACCESSION   DATNDT010000311 DATNDT010000000
VERSION     DATNDT010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3224
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1188887"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..2535)
                     /locus_tag="VN788_10050"
     CDS             complement(160..2535)
                     /locus_tag="VN788_10050"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HXU20910.1"
                     /translation="MRLLDRYIAREVVSHATLGLSVFTFIFFVPQLTKLMELVVRHTE
                     SFATVLLLIVCTLPPVLGFTLPMAVLVGVLIGLGRLSADSEIVALHASGVSLRRLLLP
                     IGAIAALGAILTLLTTFWLSPIALRTISRLEVRLLSSQAPYSVQPRVFDERFPHLVLY
                     VQDVESAALRWRGVFLASTGGIAAGIVPQAARHANESAITIAENAQIILGPGPGRIEL
                     RLGSGSTHEYDPSHPDQYNVTTFEATDIPVDLSTGALPQKRGALPIVEQPTASLVSDD
                     GPNWRAARVEFQRRIAFPAACLVFVLLGVPMGVRPRRGGRAAGLILTLILIGGYYFLF
                     VSGDHMAVQGRISPGWGVWAANIAATVLGVILLWRTENIRRPNHALAWVESLWARRKE
                     RRRAVPQPAGSNGPLNFTERRGLITRTLKANRTMGFPMLIDIYLLRQFLYYFVVLLAG
                     FVVIFDAFTLFDLLADIARNNIGVLTVVNYFRYLVPSIVYQLAPLATLVATLVTLAIL
                     AKNNEVIALKASGISMYRLVLPLILTGCLVSGGMFVLDETYLPYANQRQDALRNRIKG
                     RPAQTYFEPAQQWIFGENSKIYNYEFFDPDQRLFGGLNVFELNPATFQVRRRVFATRA
                     SWEPTEKIWVLTRGWVRDFGADGRVAEYRPFTATSLSELIEPPGYFRREVRQYSQMNW
                     RQLSNYIASLRQAGFDTARLAVQWQKKFAFPLIAAIIISLAAPFAFLVGTRGAVGGLA
                     VAVGIGVVYWATAALMEAMGSVGQLPPVLAGWGPDAIFGFIAVYFFLRMPT"
     gene            2574..>3224
                     /locus_tag="VN788_10055"
     CDS             2574..>3224
                     /locus_tag="VN788_10055"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HXU20911.1"
                     /translation="MSRVNLAILWHMHQPQYRDPATGRYLLPWTRLHALKDYWGMVKI
                     LEEFPRVHATFNIVPLLAEQIEEYVGGRFQEAWFDIAFAHAGSLQPEQKRETLERGFQ
                     VNENFINRWPRFAELASQLRSGGAEACVAHWSLRDWRDLQVLSQLAWMDEEYLAKDPV
                     VHALSEKGKNFSEEDKAGLLEKQQELLASVLLEYRRAAARGQIEISTTPYYHPILPL"
BASE COUNT          742 a          979 c          907 g          596 t
ORIGIN      
        1 ccaaaagcgg acagcatgtc aatcacaccc cgggaaggat gaacagtcct gggaaatatt
       61 ctaggtgaag tttatccaag ctgcgcagag tttcaaacgg aagttgtcaa ttgcttacct
      121 aagcggcccg cgggcacaga acggctccag gcgcgggtgt caggtgggca tccgcaggaa
      181 aaaatacacc gctataaagc cgaagatggc gtccgggccc cagcctgcaa ggaccggcgg
      241 cagttgaccg accgagccca tcgcctccat cagcgcggcg gtggcccagt aaaccacacc
      301 gattccaacg gcaactgcga gcccgccgac tgccccgcgc gtccctacaa gaaacgcgaa
      361 cggcgcagcc agagaaataa tgatcgccgc aatcagcggg aacgcaaatt tcttttgcca
      421 ctgcacagcg aggcgcgcgg tgtcaaaacc ggcttggcgc aggcttgcga tgtaattgct
      481 gagctgcctc caattcatct gggaatattg gcgcacttcg cgacgaaaat atccgggcgg
      541 ctcgattagt tcggaaaggg aagtggccgt gaacggccga tactcggcga cgcggccgtc
      601 agcaccgaaa tcgcgcaccc atcccctggt taatacccag attttttccg tcggctccca
      661 gcttgcccgg gtcgcgaaga cgcgccggcg cacttgaaaa gtggcggggt tgagctcgaa
      721 tacgtttagg ccgccgaaaa gtcgctggtc gggatcgaaa aactcgtagt tataaatttt
      781 ggagttctcg ccgaagatcc attgctgtgc cggctcgaaa tacgtttgcg ccggacgccc
      841 cttgatccga ttccgcagcg cgtcctgccg ttgattggcg tacggcaagt atgtttcatc
      901 cagcacaaac attccgcccg ataccagaca ccccgtaagg atcagtggca gaaccagcct
      961 gtacatgctg attccgctgg ccttcagcgc gatcacctcg ttgttcttgg ccaggatcgc
     1021 gagagtcact aaagttgcaa ccagcgtcgc cagcggcgcc agttgatata cgatcgacgg
     1081 caccagatag cggaagtagt tcaccacggt caaaacgccg atgttgttgc gcgcgatatc
     1141 ggcaagcaga tcgaacagcg tgaacgcgtc gaagatcacc acaaatcctg cgagcaacac
     1201 gacgaagtag tagagaaact gccgcagcaa gtagatgtcg atgagcatgg gaaaacccat
     1261 cgtgcgattc gctttcaacg tccgcgtgat aaggccccga cgctccgtga aattgagcgg
     1321 tccatttgat cccgccggct gtggtacggc tctccttctc tctttcctgc gcgcccatag
     1381 cgattcaacc cacgcgagag catgattcgg ccgccgaata ttttccgtgc gccacaacaa
     1441 aatcacgcca agcacggttg ctgcgatatt cgccgcccac acaccccatc ccggagaaat
     1501 acggccctgt acggccatgt ggtcgcccga cacaaataaa aagtagtacc cgccgatcag
     1561 tatgagcgtg aggatgagcc ccgccgcgcg cccgcctcgc cgtggccgca cgcccatcgg
     1621 cacgccgagc agcacgaata ccagacacgc cgcggggaat gcgatgcgcc gttgaaattc
     1681 gacgcgcgcc gccctccaat tcggcccgtc gtcggacacc aacgaggcgg ttggctgctc
     1741 gacaatcgga agcgcgccgc gtttctgtgg gagcgccccc gtcgaaaggt ccaccggaat
     1801 atcagtcgct tcgaaggtgg tcacgttgta ctgatcgggg tggctgggat catactcatg
     1861 cgtgcttccg ctccccaatc gcagttcgat tcgtccgggg cctggcccca agatgatctg
     1921 cgcattttcc gcaatcgtaa ttgcggactc gttcgcgtgt ctagcggcct ggggcacgat
     1981 tcctgccgcg atgccgccgg tagaagcaag aaaaactcca cgccaccgca gcgccgcgga
     2041 ttccacatct tgcacgtata atacgaggtg cggaaaacgt tcgtcgaaca cgcgcggctg
     2101 cacgctatat ggcgcttgcg atgaaagcag ccggacttcc aagcggctga tggtgcgcag
     2161 cgcgattgga ctaagccaaa atgtcgtaag caacgtcagg atggcgccca gagcagcgat
     2221 ggctccgatg ggaagaagca atcgcctcag gctgacgcct gaagcgtgca gcgccacaat
     2281 ctcgctgtcg gcggaaaggc gtcccaagcc gattaggacg cccaccaaaa cggccatcgg
     2341 aagcgtaaag ccaagcaccg gcggcagcgt gcagacaatc agcagcagca ccgtcgcaaa
     2401 gctttccgta tgccgcacca ccagctccat cagcttggtg agctgcggca caaaaaatat
     2461 gaatgtgaag accgaaaggc ccagcgtggc atgagaaact acttcgcgag ctatatatcg
     2521 atcgagtagg cgcatgggag cgcgaaacgc attctagcat tcggagccgc tgattgagcc
     2581 gcgtaaacct ggctattctc tggcacatgc accagcccca gtatcgcgat cccgccacgg
     2641 ggcgatacct cttaccttgg acccgcctcc acgccctcaa agactattgg ggaatggtga
     2701 agattcttga ggaatttccg cgcgtccacg ctacattcaa cattgttccg cttctggccg
     2761 aacagatcga ggagtacgtt ggcggacgct tccaggaagc ctggttcgac atcgctttcg
     2821 cccatgccgg gagtctgcaa ccggaacaga aacgcgaaac gctggaacgc ggctttcaag
     2881 taaacgaaaa cttcatcaat cgctggcctc gtttcgccga actcgcatcc cagctgcgtt
     2941 cgggcggggc ggaggcgtgc gttgctcatt ggagtctccg tgactggcgc gacttgcaag
     3001 tcctctcgca gctcgcctgg atggacgagg aatatctcgc caaggatccc gtcgttcacg
     3061 ctctttccga gaagggcaaa aatttctcgg aggaagataa agccggcctg ctggaaaagc
     3121 agcaggagct gctcgcctca gtcctcttgg aataccgccg agcggccgcg cgaggccaga
     3181 ttgaaatttc gactacgcct tactatcacc ccatcctgcc tctg
//
LOCUS       DATNDT010000312        14284 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_736165, whole genome shotgun sequence.
ACCESSION   DATNDT010000312 DATNDT010000000
VERSION     DATNDT010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 14284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14284
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736165"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VN788_10060"
     CDS             complement(<1..260)
                     /locus_tag="VN788_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006913759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20912.1"
                     /translation="MKASKYVLVFLIALLTIQARSFAQNVPYERLLHADAEPQNWLMY
                     GGNFQSQRFSRLKQINRDNVQTLRPVWIYQPPKPGMDEASPVV"
     gene            complement(257..1093)
                     /locus_tag="VN788_10065"
     CDS             complement(257..1093)
                     /locus_tag="VN788_10065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU20913.1"
                     /translation="MSAIRSCLIVALVFTLGSLAQNALPAWQGQQGRPGAQSAIVADA
                     DKGRDLFQANCSGCHGIDAGGGDGPDIQQAPASLGDANVANIIRKGVPGTPMVGFFSI
                     GDAGAADIVAYIRKLGSTITSETTKGDPKKGEEIYKSSGCPACHMIAGQGGEIGPELT
                     SIGVKRGPTNLHQRLIDPGVNLPKEGNVGDRGKWTLYLLYRAVTNDGRIVEGIRMGED
                     SFSIVLKDVNGNFHALYKPNLRSLEKEPSKSLMPSFKDTLSSAQMDDLVAYLVSLKGA
                     TP"
     gene            complement(1166..2797)
                     /locus_tag="VN788_10070"
     CDS             complement(1166..2797)
                     /locus_tag="VN788_10070"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU20914.1"
                     /translation="MKATKFFLLLLLFCLCGPSGSSAQDAPYMRLLHSDSEPQNWFMY
                     GGDYQSKRFSFLKQITPQNVANLRPAWIYQRSRPGEMIEDSPVVVDGIMYIVEPPSTV
                     TALDARTGLAIWSWTPALPRVVKVDGYHATSRGVAVLDDMVYVGTIDCHLVALDRITG
                     AVRWSVTLGDNKINYGLTAAPMAIDGKIIIGPSGSEDGVRGFIDAYDAKTGKHLWRLY
                     TVPNPGEPGSETWGGGQILGGAAWNKGSYDPELNLIYWGTGNPAPDYNGDGRPGDDLY
                     TCSLLAINPDTGKVKWYFQYTPHDTHDWDGTQVPVLFNATLDGKPRKLLSVANRNGFY
                     YVLDRVTGEFVTGVPFVKETYAKGLDAKARPIVLPNTDPGLEPVLVYPSNNGGVDWVS
                     PAYNPLSKTFFVNAREMGAYFAKSKLPDKPLVVGTGMVMGGRGNSYLSGDDSYGAIRA
                     LDVTTGKRKWEFKMQAPSWVAPLATASNLLFSADDEGDFFALDSNTGKVLWHFRTGGS
                     FISSDTRSSPITYEVDGKQYVEAADGDTFIAFALP"
     gene            complement(2794..3645)
                     /locus_tag="VN788_10075"
     CDS             complement(2794..3645)
                     /locus_tag="VN788_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU20915.1"
                     /translation="MSAIQSLAVAILILILCGLPQSLLAASPNFKQQQQAGPGAQADL
                     DVGRQAYVANCSRCHGLYATGGDGPNIQRAPITLGNDGVIKIIKDGVPGTMMTAFNLD
                     DAQIAAIIAYIRSLGRRNEPAMPTRGDKLKGQAVYDGNGCSGCHTIDGQGGSLGPELT
                     QIGSLRQPDMLRQTLLDPGNALPTTRPNRDRGKWLLYTIFRAVQKDGRVVEGMRVSES
                     TFAIVLEDAKGNFHSLSKANLRSLEKEPGKTYMPSFKDTLSAAQVDDIVAYLSSLRDS
                     SDLRNTQ"
     gene            3887..5086
                     /locus_tag="VN788_10080"
     CDS             3887..5086
                     /locus_tag="VN788_10080"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HXU20916.1"
                     /translation="MNLKWSRAQVVLIAFLFVIICDLPGTQAQTAKKSSVLVLDPAVE
                     QLISPSAKLEPVKLDYFGRLEGPVWVPGPGKSGYLLFPEVAGNLIYKWQPSCAKYPCG
                     TKGNLSIYATNAAFTGTPQQFNDVGFITYNGRLYNIHVGPVGLALDPQLRLIVTAFGD
                     HEMVRYDKDMKRTVIASSWQGMHLNCPDDTVVKSDGTIYWTDGPQTCYRQRNPFDFNA
                     SNQQIKYPGLYMIKDGENQLLDKTNAGVNGVALSPDEKTLYVTGGHDTLLKYDVQPDD
                     TVTNRRLFIDMLASAHITKVQRETADAIFPDGVRVDSRGDIWTPGPGGIWIISPEGKH
                     LGTILPPPDPELLKYQVFCSLAFGDDGKTLYIDGNTDLWQIPVKVCGSSVPPFTCNND
                     KNYPLSH"
     gene            5222..6094
                     /locus_tag="VN788_10085"
     CDS             5222..6094
                     /locus_tag="VN788_10085"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU20917.1"
                     /translation="MWNIRSTRVPALAIVLGALVIGFSPISGSGQEQRSQGSPAVGRN
                     QRTGGTPPEGNATKGHDIFVNNCAVCHGVDATGGEGPNIQRTPETLGDAAVQTIILRG
                     VPGTEMPSFFMMLNASDVSDIISYLRQLANTATAGTVTGDPAQGEALFKSNGCLGCHM
                     IDGQGGDLGPDLSRVGSMRGPSYLHTKLLEPGADLPTTGAEGDRGKWTRYLMFRAVTK
                     TGQVVGGMRVGENSFNVVLMDAQGNFHALYKPDLKSLDQEPGKSLMPSYKGILSQAQL
                     NDLVAYLSNLKGAQ"
     gene            6091..7710
                     /locus_tag="VN788_10090"
     CDS             6091..7710
                     /locus_tag="VN788_10090"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU20918.1"
                     /translation="MKWLGYLAVVVLTVIGVSELSFAQNVTYKRLLHADDEPQNWLMS
                     GGNYQAWRFSPLKQINTANVGTLKAAWIYQPAQSGQQEASAVVVNGIMYITEAPSGVT
                     ALDAKYGTKIWSWSPKLPRVIYHVGSHLDNRGIAILGNTIYVGTVDAHLVALDAKTGA
                     MKWDVHIADNKENYGINSAPLVINEKVIVGIGGGDGGARGFVDAYDAKTGQRLWRVYT
                     IPAQGDPVAKTWGDDMTGLGGGGTWVTGSYDASLNLLYWGTGNPAPSFNRAGRPGDNL
                     YTCSLLAIDPDTGKMKWYFQFTPHDTHDWDSAQTPVLFDATVDHEQRKFVAMANRNGF
                     YYVLDRVTGKFAKGVPFVKVTWAKGLDENGRPIYNGDPDPTPKGTLVYPAVMGGADWT
                     APAYSPLTKMFYVNAHELGAYFYSGQPRLNPNGPPGYGGGGQNALSGDKAYGALRALD
                     SVTGKLIWQYRLLAPAWVSPLVTAGSLVFAGTDEGNFFALDAEKGKLLWEFNLGGSGN
                     VFRTNSITYAVNGKQYVLAVAGNDFVAFTLP"
     gene            8145..9392
                     /locus_tag="VN788_10095"
     CDS             8145..9392
                     /locus_tag="VN788_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20919.1"
                     /translation="MNDKYSDATVSKTDRRGFIKGAAAAGLLGLAPAVTSAAAPQATV
                     EDPQKPAGLGSMGLPDGRFPMMYETSVPEAVRVLTQYFKALSQRDLRALSQTVQYPFA
                     SYEGTDPVVIETAEELIAKAPPSMNMSENPERFTDHDGYIKAGSYDVFGGIEIFNSDN
                     ARVNLALTYDRYGKDGKRMLRCEGIYCVTNNDGKWGIQLMSTIFTPSDMVGTVYEDTI
                     MACARLREDHCLAFQVNEGNDFARTVQFGKVAAMADVSYSLLQNNAINGNPMATYKVK
                     GVKTRLITREVSEESVLGPGPAPISANLNNPGNPQTRGNRGGNGDFSRSARELYARIG
                     HGIKWGFVSGAPIGHRVVHHSMDKAHMYSGVTRFTPAGEEINTTMELDVVTYKKSHWG
                     IPGAPLTFAYITTHDRVNDLDSV"
     gene            9478..10737
                     /locus_tag="VN788_10100"
     CDS             9478..10737
                     /locus_tag="VN788_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20920.1"
                     /translation="MEGVNKGRPGLVAERDAAEVNESANPAQTVAQDPWAPLEMKEDP
                     KAPAGLGPRATPDLRFSICYFPSVPQAARVMTRFFHALAQRDLRMMADTLQFPFATWE
                     GTDRFVINTPEELIAKAPPSLNMSLDPVRFTDHDGYLKPGAYDVFGGMEFLNSTPIES
                     VLSMYYDRYDKNGKRMLRCEGVYDVTNNDGKWAIQQMSTIFTPSEMVGTVYTDTITAA
                     KRLRINHDLAYQVEDNDGVWGRVSPPVWVPQGAVPPAAPTGGANGAAPTTSGTQNRSR
                     AGGAGTPWQDAPNGNAAIMRHFRFKGVKSRIQPETQQPAPPSGPGEWVERKANIDFPQ
                     TLKDVSAAGAGNWGFPVGLLPCTRVLHNTENKAHLYSGVTRYNVWGEEISNSVGVCII
                     TYKLGRWSWDGSLGYICNHDRANDVHA"
     gene            10788..11870
                     /locus_tag="VN788_10105"
     CDS             10788..11870
                     /locus_tag="VN788_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20921.1"
                     /translation="MTRRGNEANAQPVPAQESYDQDPKAPAGLGPKASPDQRFELCYG
                     PPVAEGVRVLTEYFVALSARDPKAMAETMHFPFGSYEGTEVVVVEAPDDLVGKAPPSM
                     NMSEHPDRFTDHDSFLKPGAYDVFDGIEVLNTNPVAANLSLSYDRYDKDGKRLLRCDG
                     VYCVTNNDGRWAIQLASTIFTPSDMVRRTYPDTVEIAKRLRINHDLAFQVRDDQGVWA
                     RVRQHGKSAGIAGGGGWANAPNGNVMLNYRVKGVKNRLRINEYTPESLANMHTDFART
                     LREVAGAGVGYWGFPIGILPDTRVIHSTVDKAHMYSGVTRFNVQGEEISVSAEISIIT
                     YKKGRWGWDGSLAYTIPHDRANDIHS"
     gene            12277..13392
                     /gene="tcuB"
                     /locus_tag="VN788_10110"
     CDS             12277..13392
                     /gene="tcuB"
                     /locus_tag="VN788_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate utilization 4Fe-4S protein TcuB"
                     /protein_id="HXU20922.1"
                     /translation="MAGLDLFEEANRQLTICNACRYCEGFCPVFRAIEIRRSFTENDV
                     RYLANLCHDCRACHQACMYTAPHEFAINIPKVMSEARVESYEHWSWPTFLGRSFSNTA
                     KGVLLGCTAAVIVYVVALFLIPRDHLFATHLGPGAFYRVVPYAAMVIPAVILFLYGGA
                     IWLQGSVSFWGESDSTSLRQHSGIAPVLRAVRDAFALKYLGGGGPGCSYPDETPSAIR
                     RIFHSLVFWGFLLDFASTTFAFIYEDFLHVLPPYPILSIPVISGSVGGLGLIVGTAGL
                     LWFKMKSDRSLSAAPAYSLDYAFLVFLGLTALTGMLTLMLRSTSALGTILVLHLGMVA
                     ALFITAPYGKFVHFVYRSFALIRYQVEAESSQAEAGN"
     gene            13735..>14284
                     /locus_tag="VN788_10115"
     CDS             13735..>14284
                     /locus_tag="VN788_10115"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU20923.1"
                     /translation="MPGPVSPSPLIATRPRRKAPAGAVDCHFHIFGPADRYPIHPTSL
                     YTPSPQANIKNYQAMADTLGIQRMVIVNPTPYGTDTRCTIESIGIFGKDRAKAVAVVN
                     ETVSDAELRDLDKQGFCAARINSLMTNSTPVSEMQTVIRRIAPLGWHLEIYVQGEELP
                     GLEKTILSLPVTVAIDHMGRIPT"
BASE COUNT         3202 a         3915 c         3975 g         3192 t
ORIGIN      
        1 acaacgggag acgcttcatc catccccggt ttgggaggtt gataaatcca cacgggccgg
       61 agcgtttgga cgttgtcgcg attaatttgt ttgagccggg aaaatcgttg cgattgaaaa
      121 ttaccgccgt acatcagcca gttttggggt tcggcatctg catgcagaag ccgttcatac
      181 ggtacattct gcgcaaacga tcgcgcctgg atcgtcagaa gcgcaatcag gaatactaga
      241 acgtatttag aagctttcat ggtgtggcgc ctttcaaact cacgagataa gcgaccaagt
      301 catccatttg tgccgaagac agagtgtctt taaagctggg catgagactt ttcgagggtt
      361 ctttttccaa ggatcgcaga ttgggcttgt agagagcgtg gaaattccca ttgacatcct
      421 tcagcacaat tgaaaatgag tcttcaccca tgcggatgcc ttcgacgatg cgaccatcgt
      481 tcgtgacggc gcgatatagg aggtatagag tccatttgcc gcgatcgcct acgttcccct
      541 ctttcggcaa atttacgccg ggatcaatca atctctggtg caagttagtg ggaccgcgct
      601 tgacgcctat ggaagtcaac tccgggccga tctctccgcc ctgtccagca atcatgtggc
      661 aagcagggca gccactcgac ttgtaaatct cttcaccctt tttgggatca cccttggttg
      721 tctcggaagt gatcgtgctg cccaactttc ggatataagc cacgatgtcg gcggcaccag
      781 catcgccaat actgaaaaaa ccaaccatcg gcgtgccggg aacccctttg cgaatgatgt
      841 tggctacatt tgcatcaccc aaagaagctg gagcttgctg aatatcgggc ccatcaccac
      901 cgcctgcatc gattccatgg catcccgagc agtttgcttg aaacagatct cttcctttat
      961 cggcatccgc tactatggcg gattgcgctc cgggccttcc ctgctgacct tgccaagcgg
     1021 gaagcgcgtt ttgagcgaga cttcccagtg tgaaaaccaa agcgacaatc aaacaggatc
     1081 gaatcgcact caccacgcac ctccgcgttc ggcagaacag caccggcttc gagccgtcgt
     1141 gccacaagaa gctatgcgta actatttacg gcagggcgaa cgctatgaat gtgtcgccgt
     1201 ccgcagcctc cacgtactgc ttgccgtcca cttcgtatgt gatcggactg gacctcgtat
     1261 cggagctaat gaaggaacca cctgttcgaa aatgccacag taccttgccc gtgtttgagt
     1321 caagagcaaa gaagtcacct tcgtcatctg ctgaaaagag caggttgctc gcggtcgcca
     1381 ggggagcaac ccaggaaggg gcttgcatct taaactccca ctttcttttt ccggttgtga
     1441 catcaagggc gcgaatagcg ccgtatgaat catccccgct gaggtacgaa tttcctcgtc
     1501 ctcccataac catgcccgtc ccgaccacca gcggcttatc tgggagcttg gattttgcaa
     1561 aataagcgcc catttcgcgc gcattaacga agaaggtttt tgacagtggg ttgtacgccg
     1621 ggctgaccca gtccacgccc ccgttattac tgggatagac gagtactggt tccaagccgg
     1681 gatcagtatt cggcaatacg atcgggcgtg ccttcgcgtc aagccccttc gcatatgtct
     1741 ctttaacgaa gggaacgcca gtaacgaact cgcccgttac acgatcgaga acgtagtaaa
     1801 agccgttgcg attggccacg gaaagaagtt ttcgcggttt gccgtccaag gtggcgttga
     1861 acagcaccgg aacctgcgtg ccatcccaat catgcgtgtc gtgcggcgtg tattggaaat
     1921 accattttac ttttccagta tccggattga tggcgagaag agagcacgta tagaggtcat
     1981 ctcctggcct gccgtctcca ttgtaatcag gggccggatt cccggtgccc caatagatca
     2041 ggttcagttc cggatcgtag gaacccttgt tccatgcggc tccgcccaga atctggccgc
     2101 ctccccaagt ctccgagccg ggttctccgg gatttggaac cgtatacaat cgccatagat
     2161 gcttcccggt tttcgcatcg taggcgtcga tgaatccccg aacgccgtcc tcgctgcccg
     2221 acggcccgat gatgatcttt ccgtcaatcg ccatgggtgc cgccgtcaag ccgtaattta
     2281 ttttgttgtc cccgagggtc acactccagc gaacggcgcc ggttatccga tccaacgcga
     2341 ccagatgaca atcaatcgtg ccaacgtaga ccatgtcgtc aagcacggcg actccccgac
     2401 ttgtcgcatg atagccgtct accttgacta cccttggcag cgccggcgtc caactccaga
     2461 tcgccagtcc tgtgcgggcg tcgagagccg tcactgtgct gggcggctcg acgatgtaca
     2521 tgattccgtc gactacgacc ggagagtctt cgatcatctc gcctggccta ctgcgttggt
     2581 agatccaagc gggccgtaaa ttggcaacgt tctgcggggt gatttgtttg agaaacgaaa
     2641 agcgtttcga ttggtagtca ccgccgtaca taaaccaatt ttgcggttcg ctatcggagt
     2701 gcagaaggcg catgtagggc gcatcctgtg cggaggaccc agagggaccg cagaggcaaa
     2761 acagaagcag caataggaaa aatttagtgg ctttcattgc gtgttcctca ggtcagatga
     2821 gtctctcaag ctagaaagat atgcaacaat gtcgtcaact tgcgcggcag acaaggtgtc
     2881 cttaaagctg ggcatataag tcttgccagg ctccttttcc agggagcgca ggtttgcttt
     2941 cgagagcgag tgaaaattgc ccttggcgtc ctccagcacg atcgcgaatg tagattcact
     3001 aacgcgcatt ccttccacaa ccctgccatc tttctgcacg gcacggaaga tcgtgtacag
     3061 gagccacttt ccccggtccc tgttaggcct cgttgtgggt aaggcattgc ctggatccag
     3121 caatgtttgg cgtagcatat ccggttggcg caaactaccg atttgagtta gttcaggacc
     3181 caagcttccg ccttgtccgt cgatagtgtg gcagccagag cagccatttc cgtcgtaaac
     3241 tgcttggcct ttaagcttgt cgcctcgtgt aggcattgct ggctcattcc gccgacccag
     3301 cgatctgata taggcgatga tggccgcaat ttgggcgtcg tccagattaa aagcagtcat
     3361 catggtaccg gggacgccat ccttgattat cttaatgact ccatcgttac ccaacgtaat
     3421 tggagccctt tgaatattgg ggccatctcc gccggtcgca tacaagccat ggcatctcga
     3481 acagtttgcg acataggcct gcctgccaac atcgaggtcc gcctgcgcac ctgggccggc
     3541 ctgttgctgt tgcttgaaat tgggagaagc ggccagcagg ctctggggca atccgcacag
     3601 aatgagaatc aatatggcaa cggccaagga ttgaattgca ctcattttag ttccccgcaa
     3661 aatgggatca tagttccgca tcgtgaaacg gcgtcacgtt aaccagcgag gctgcttgat
     3721 gtcaatataa atctcgcgtg ctaccgataa ctcgacgcgc tttttcttga cgggcagttt
     3781 ctggcatggt acaaggcccg cgcggggttc tcatgcgcgc gtcggcgtgt cgcatagagg
     3841 aacagaggcg atatattcct ggctccatct aaagggggat acaagagtga atctcaaatg
     3901 gtctcgtgct caggtcgtac tgatagcatt tttattcgtt atcatatgcg atctgccagg
     3961 gacgcaagcg cagaccgcta aaaaatccag cgttcttgta ctggacccgg cggtcgaaca
     4021 attaatctcc ccaagtgcca agctcgagcc tgttaagctg gattatttcg ggcgtttgga
     4081 aggcccggta tgggttcctg gaccgggcaa gtccggttac ctcctgtttc ctgaggtagc
     4141 cggcaatctc atttacaagt ggcagcctag ctgcgcgaag tatccgtgcg gcacgaaggg
     4201 aaacctttcc atctacgcga cgaacgcagc cttcaccgga acgccgcagc agttcaacga
     4261 tgtgggcttc atcacctaca acgggcggtt gtataacatt catgtcggcc ccgttggtct
     4321 cgccctggac ccccagctcc ggctcattgt gacggcattc ggagaccatg agatggtgcg
     4381 ctatgacaag gatatgaagc gcactgtgat tgcctctagt tggcagggaa tgcacttgaa
     4441 ttgcccggac gacactgtcg tcaaatcaga cggcacgatt tactggacgg atgggccgca
     4501 gacctgctac cgccaacgga accctttcga tttcaatgcg tcgaatcagc agattaagta
     4561 tccggggtta tacatgataa aagacgggga aaaccaactt ctcgataaga cgaacgccgg
     4621 agtgaacggc gtggcgttat ctcccgacga gaagactctc tacgtcactg gtggtcatga
     4681 cacccttctg aagtacgacg tccagcccga cgataccgtc actaatcgcc gtttatttat
     4741 cgatatgttg gcaagtgccc acattactaa agtacagcga gagacagccg atgcgatttt
     4801 tccggatggt gtccgtgtgg attcccgggg agacatctgg actccgggtc ccggcggtat
     4861 ctggattatc tcgccggaag gcaagcatct cggcacgatt ctccctccgc ccgaccccga
     4921 acttctcaag taccaagtct tttgcagctt ggcgtttgga gacgacggca agactctgta
     4981 catcgacggc aacaccgatc tgtggcagat tccagtgaag gtctgcggct caagcgtgcc
     5041 tccgtttacg tgtaacaacg acaagaatta cccgctcagc cattagttgg tctcccgtgt
     5101 ggaccattgg tgtgtcacgc gatcgaagat aggtagggcg tcccggaccg gtacgaagga
     5161 taggtcaggc cctgggaaaa cgtgtcgcta cgcctggtta gagcgtgagt ggagagctca
     5221 aatgtggaat atccggtcaa cacgtgtccc cgctctagcg atcgttctgg gtgctctggt
     5281 gatcggtttc agcccgatat ccggctcagg gcaagagcaa cgatcgcaag gctcgcctgc
     5341 cgtgggccgc aaccagcgta ctggcgggac gccgccagag ggaaatgcga ccaagggcca
     5401 cgacatcttt gtgaacaact gcgctgtctg ccacggtgtg gatgcgacag ggggcgaggg
     5461 ccccaacatt caacgaacgc cggagacctt gggtgatgcg gccgtgcaaa ccattatctt
     5521 gagaggggtc cctggaaccg agatgccgag cttcttcatg atgctcaatg catcggatgt
     5581 ttcggacatc atcagctatc ttcgccaact tgccaacacg gcgaccgctg ggacggtcac
     5641 cggcgaccct gcgcaaggcg aggcgctctt caagtccaat ggttgtcttg gttgtcacat
     5701 gatcgatgga cagggaggcg acctgggtcc tgacctttcg cgcgtcggca gcatgcgtgg
     5761 tccaagctat ctacacacta agctgctaga gcccggagca gaccttccga cgactggagc
     5821 cgagggagac cgcggaaaat ggacgcgtta tctgatgttt cgcgcggtga cgaaaaccgg
     5881 ccaggttgta ggtggtatgc gagtcggcga gaactccttt aacgttgtcc ttatggatgc
     5941 gcaaggaaac ttccatgcgc tttacaagcc cgacctgaag tctctcgatc aagagccggg
     6001 aaaaagcttg atgccaagct ataaagggat tctgtcgcaa gcgcaactga atgatttggt
     6061 cgcgtacctt tccaacttga aaggcgcaca atgaaatggc tggggtattt ggcagttgtg
     6121 gttctcactg tcataggggt ttccgagctt tcatttgctc agaatgtcac ctataaacgg
     6181 cttctgcacg cagacgacga acctcaaaac tggcttatgt ccggcgggaa ctatcaagcc
     6241 tggcggttct ccccactaaa acaaattaac acggcgaacg tcggaactct caaagctgct
     6301 tggatctacc aacccgctca atcgggacag caggaagcct cggccgtggt agtaaatggc
     6361 attatgtaca ttaccgaagc ccccagcggc gtgacggctc tggacgccaa gtacggtacc
     6421 aaaatatgga gttggtcccc caagctaccc cgcgtgattt atcacgttgg ctctcatttg
     6481 gataaccgtg gcatcgcgat tttgggtaac acgatatacg tcgggacagt cgatgctcat
     6541 ctggtggctc tggacgcaaa gacgggcgcc atgaagtggg acgtccatat tgctgacaac
     6601 aaggaaaact acggcatcaa ctcggctccg ttggtgatca acgaaaaggt cattgtcggc
     6661 atcggcggag gggatggtgg tgcccgaggg tttgtggatg cctacgacgc gaagaccggc
     6721 cagcggcttt ggcgcgtcta tacaattccc gcgcaggggg atcccgtcgc gaaaacctgg
     6781 ggcgatgaca tgacgggact aggaggtggc ggcacttggg ttaccggatc ctacgatgct
     6841 agcctgaact tgttgtattg gggcactggg aatcccgcgc cctccttcaa cagggccggc
     6901 cgccccggcg acaacctgta tacgtgctct ctgcttgcaa ttgatccgga caccgggaaa
     6961 atgaaatggt acttccagtt tacgccccac gatacgcacg attgggattc tgcccagact
     7021 cccgtgctct tcgacgcaac cgtggaccac gagcaacgga agtttgtcgc gatggccaat
     7081 cgaaatggct tttactatgt tctcgaccgt gtcacgggaa aatttgcgaa gggggtcccc
     7141 ttcgtgaagg tcacatgggc gaaggggctc gatgagaatg gacggcccat ctacaatggg
     7201 gacccggacc caacaccaaa ggggacgctt gtttacccag cagttatggg tggagcggat
     7261 tggaccgcac ccgcttacag ccccctgacg aaaatgttct atgtaaatgc ccatgagctg
     7321 ggcgcctatt tctattcagg ccaaccaagg ctgaatccaa acggtccgcc gggctacggc
     7381 ggcggtggac aaaatgcact cagcggggac aaggcctatg gggctcttcg agctctcgat
     7441 tccgtaaccg ggaagttgat atggcaatac cgcctcctcg cccctgcatg ggtaagtccg
     7501 ctcgtaacgg ctggcagcct cgtgtttgcc ggaactgacg aaggcaattt cttcgccctc
     7561 gacgctgaga agggcaagct gctgtgggaa ttcaacctcg gcggcagtgg aaacgttttt
     7621 cggacgaact ccatcaccta tgccgtcaat ggtaagcagt acgtgctggc cgtggccgga
     7681 aacgactttg tagccttcac cctgccctaa cccgcgtgct aagggggtac gcagttccgc
     7741 cgagcgcagc atttgttaag tttgcagcgt tcgtggagga ctgctgaatt cctttgtaga
     7801 attgtggaaa gcttggcact ccaaaacctc aacaatcgtc cttgggaaaa acaaacacag
     7861 tggacataaa gctgcaaacg gcgctaccga aagccacttt tgcagcggta ttgagcgaag
     7921 attagcggct gatgtccgcg gcgcgacttg attagcgttg acagaaatcc gcgcaagtga
     7981 taactaacac ctgtcgtgcg tgtggaatca agttccgcaa tgcggaatct ggtttgttct
     8041 cacctttaca gccggcgcga atgaactctc cttgcgagcc tgcgagaagg atttagactt
     8101 tttcacagct tcctctcacc aacgaagttc aaaggaggac cggcatgaac gacaagtact
     8161 cggatgccac ggtcagtaag accgaccggc gaggcttcat taagggcgct gctgcagcgg
     8221 gattactagg cttggcaccc gcggttacct ctgctgctgc gccgcaagcg accgtcgagg
     8281 atccccaaaa gccagcgggc ctgggttcta tgggcctgcc agatggccgc tttccgatga
     8341 tgtatgaaac gtcggtcccg gaagctgttc gagtcctgac ccaatacttc aaggcgctaa
     8401 gccagcgcga tctgagggcc ttgtcgcaga ccgtgcaata ccccttcgcg agctatgaag
     8461 gaactgaccc tgttgtgatt gaaaccgcgg aggaactgat cgccaaggct ccgccctcga
     8521 tgaacatgtc ggagaatccc gagcggttca ccgaccacga tggctatatc aaggctggct
     8581 cctatgatgt gttcgggggc atcgagatat ttaactccga caacgcgcgt gtgaaccttg
     8641 ccctgacgta tgaccgctat gggaaggacg gtaagcggat gctgcggtgc gagggcattt
     8701 actgcgtcac aaacaatgat ggcaagtggg gcattcaact catgtccaca attttcaccc
     8761 cctccgatat ggtcggcacg gtttacgagg acacgatcat ggcgtgtgca cgcctgcggg
     8821 aagaccattg tctggcgttc caagttaacg agggaaatga tttcgcccgc acggttcagt
     8881 tcggcaaggt agcggctatg gcagacgtca gttattcgct actgcaaaac aacgccatca
     8941 atggtaatcc catggcaacg tacaaggtca aaggcgtcaa gacccgcttg atcaccaggg
     9001 aagtgagtga ggagagtgtt ctcgggcctg gccccgcgcc tatttccgcc aatctgaaca
     9061 atcctggcaa tccgcaaact cgaggaaatc ggggcggcaa cggtgacttc tcacgatcag
     9121 ctcgcgagct atacgcccgc atcggtcacg gcatcaagtg gggcttcgtc tcgggagcgc
     9181 cgataggcca tagggttgtg caccactcga tggacaaagc gcacatgtat tcgggcgtta
     9241 ctcgctttac acccgccggg gaagaaatca ataccaccat ggaactggac gtcgtgactt
     9301 ataagaaatc gcactggggc atccccggtg cgccgctcac gttcgcatac atcactacgc
     9361 acgatcgcgt gaacgacctg gatagcgttt gaccctgcag ttttccagag ggctcggcta
     9421 gcggctcggt tgcgacgatg aaggccagag taaatcgaaa aagggagctc gaaaataatg
     9481 gaaggcgtca acaaaggtcg accgggactc gtggcggagc gggatgctgc ggaagtcaat
     9541 gagtcggcga accctgccca gacggtcgcg caggatccct gggcccctct agaaatgaag
     9601 gaggatccga aggcacccgc tggtttggga ccaagggcca caccggacct tcggttctcg
     9661 atctgctatt tcccttccgt gccgcaagct gcaagagtga tgacgcgatt ctttcacgcg
     9721 ctagcccagc gcgatctaag gatgatggcc gatacgctgc aatttccgtt tgccacttgg
     9781 gagggtacgg accgcttcgt tatcaacacg ccggaagaac tgattgctaa ggcgcccccg
     9841 tctctgaaca tgtcacttga tcccgttcgg ttcaccgacc acgacggcta tttgaaacca
     9901 ggagcgtacg acgtttttgg aggaatggaa tttctcaatt ccaccccgat cgagtcggtc
     9961 ttgtctatgt attacgatcg ctacgacaag aatggaaagc gcatgctccg gtgcgagggt
    10021 gtttacgatg tgacaaacaa tgatgggaaa tgggccattc aacagatgtc cacgatcttc
    10081 acgccctccg aaatggtcgg cactgtctat acggacacca tcacagccgc gaagcggctc
    10141 cgtatcaatc acgacttggc atatcaggtt gaagataacg atggagtttg gggtcgcgtg
    10201 agtccgccgg tctgggtacc gcaaggtgca gttccaccag cggctcctac gggcggcgct
    10261 aacggcgccg ctcctaccac gtcgggtacg cagaatcgga gccgtgccgg gggtgccgga
    10321 acaccgtggc aggatgctcc gaacggaaat gcagccatca tgcgccattt ccgattcaaa
    10381 ggagtaaagt cgcgcatcca acccgaaact cagcagcctg ccccaccatc agggccggga
    10441 gaatgggtgg aacgcaaggc gaatatagat tttccccaaa ccctcaaaga cgtctctgcc
    10501 gcaggggcag gaaactgggg cttccccgtc ggtctccttc cctgtacccg cgtgcttcac
    10561 aacaccgaga acaaggcaca cttgtattct ggcgtcaccc gctacaacgt ttggggtgaa
    10621 gagatctcca acagcgtcgg agtttgcatc atcacgtaca aattgggtcg ctggtcttgg
    10681 gacggaagcc tcggttatat ctgcaaccac gaccgggcca acgacgttca cgcttgatga
    10741 gccgcgccgg gagcgcccgg acgaagccta gcaaagggag agcaggcatg acacgcagag
    10801 gaaacgaagc aaacgcgcag ccggttccgg cgcaagagtc gtacgaccaa gacccgaaag
    10861 cacctgccgg tttggggccg aaggcctcgc ccgaccaacg atttgaattg tgctatggcc
    10921 ctccggtggc cgagggcgtg cgtgtcttga cagaatattt cgtggcactc agcgcccgcg
    10981 atcccaaagc gatggcagag acgatgcact tcccctttgg cagttatgag ggcaccgaag
    11041 tcgttgtcgt ggaagcgccg gacgatttgg tggggaaggc gccgccatcg atgaatatgt
    11101 ccgagcatcc cgatcggttc accgatcacg atagcttcct gaagcctggg gcctatgacg
    11161 tcttcgacgg catcgaagtg ctcaacacca acccggtggc tgcaaacctt tccttgtcct
    11221 acgaccgcta cgacaaagat ggaaagaggt tactgcgctg cgatggcgtg tactgcgtca
    11281 caaacaacga tggacggtgg gccatacaat tagcatcgac catcttcacg ccctcggaca
    11341 tggttcgcag aacgtatccc gacactgtcg agatcgccaa gcgccttcgt atcaatcacg
    11401 acctcgcctt ccaggtccgt gatgaccagg gcgtgtgggc tcgcgtccgc cagcatggca
    11461 agagcgccgg tattgcgggt ggcggcggct gggcgaacgc tcccaacggc aacgtaatgc
    11521 tgaactaccg tgtgaaggga gtcaagaacc gcctgcgcat caatgagtat acgccggaga
    11581 gcctggccaa catgcatacc gacttcgcgc ggacactccg tgaagtcgcc ggtgccggcg
    11641 taggttactg gggattcccg atcggcattc tccctgacac acgcgtcatt cattccacgg
    11701 tggacaaggc gcacatgtat tctggcgtga cgcggtttaa cgtgcaagga gaagagatct
    11761 cagtcagtgc ggaaatctcc atcatcacgt ataaaaaggg ccgctggggg tgggacggct
    11821 cgctggcgta cactattccg cacgaccggg caaacgacat tcactcgtaa ggcatgaaat
    11881 tgtgattgga agctacgagc tgctagcagc gtgtgagcat agagtatccc catgttttca
    11941 actgattctc ggtgctgatc cgacgctctc tcgcactcaa ctcaggttgg ggcgagtgga
    12001 ggatacacgg ttgacacttt cttaagattc ctttacattc gttccgaaac agtaggggaa
    12061 tgccgtttct ctatgaagaa tgcatccgaa ttggtaacca atatacgcga tgataatact
    12121 gtcgcgaaca gtcggtcgta ttcgtgctcc acagaagaca ttcacggctg cctcgaaccg
    12181 atccgctttc ggcccggaac tcgtcgtgcg cggtataccg tacagtgatg cgaagacact
    12241 tgccactctg ctggctcaag agggacgcgg tcacgaatgg ccggacttga tctttttgaa
    12301 gaggcgaacc gtcaactcac gatctgcaat gcttgccgct attgtgaggg cttttgtcca
    12361 gtttttcgcg cgattgagat acgaaggagc tttacggaga atgatgtccg ctacttagcg
    12421 aacctctgtc acgattgccg ggcctgccat caagcatgca tgtatacagc gcctcacgag
    12481 tttgccatta acattcctaa agtcatgtcg gaggcgcgtg tggagagcta cgagcattgg
    12541 agctggccga cgttcttggg ccgctcgttc tccaacaccg ccaaaggcgt gcttttggga
    12601 tgtacagcgg cagtgatcgt ttacgtggtg gcgctcttcc tgattccgcg cgatcaccta
    12661 tttgcgactc acctagggcc tggtgctttc tatagggttg tcccttacgc ggccatggtg
    12721 attccggcgg taatcttgtt tctttatggg ggtgcgattt ggctacaggg tagtgtaagt
    12781 ttctggggtg agtctgacag cacttcgttg cgccagcact ctggtatcgc tccggtgctt
    12841 cgtgcagtgc gcgatgcatt cgcgctgaag tacctcgggg gcggcgggcc gggctgctca
    12901 tacccagatg agaccccttc ggcgatccgt cgaatctttc attcgctcgt cttttggggt
    12961 tttctgttgg atttcgcctc aacaacgttc gctttcattt atgaggactt tctgcacgtt
    13021 ctgccgccct atccaatcct gagcattccg gtaatcagcg gcagtgtcgg tggtctggga
    13081 ttgattgttg gcaccgcagg tttgctctgg ttcaaaatga aaagcgatcg atctttatcc
    13141 gctgcacctg cctatagtct tgactacgca tttctcgtgt tcctaggtct caccgctctt
    13201 actgggatgc ttacgcttat gctgcgctca acgtctgcgc tcggaacgat tctcgttttg
    13261 cacctaggga tggtggctgc gctattcatc accgcgcctt atggaaaatt cgtccacttc
    13321 gtatatcggt cttttgccct aattcgctat caggttgagg cagagagttc gcaggccgag
    13381 gccggaaact aatgacgatt ggtttcatcg ggaaagcagt ctgatgccgc tgaagtcgct
    13441 aggctcgcga cgaaaaagtt gctttggaag aaaggcgagc tacggacatc gagatccgtc
    13501 gttcgccagt aaccgactaa cagcttcgct cgctgaagac catcacatag tcgaggcttt
    13561 gatatccgtt gtggcggcac tcgtgagcca aagcattatg ttcaaccagc agtctcattg
    13621 ctcgcctcgt gaacggtggt gcgtcacagt tccgtgtcga ccctcggccg cgtaacaaag
    13681 acagcagccc ttgtgtctgc ggccagagca catctgaaac ttgaaaggaa ctcaatgcca
    13741 ggaccggttt ctccttctcc cttaatcgct acaaggcccc gccggaaggc tcctgcgggt
    13801 gcagtcgatt gtcattttca tatctttggt cccgcagacc ggtatcctat tcacccgacc
    13861 agcctttata cgccgtcccc gcaagcgaat atcaagaact atcaggccat ggcggatacg
    13921 ttgggcatcc agcgcatggt gatcgtaaat cccactccgt acggcactga cacccgttgc
    13981 acgattgagt cgatcgggat attcgggaaa gatcgcgcaa aggcggttgc ggtcgtcaac
    14041 gaaactgtta gcgacgccga actgcgcgat ttagataaac aggggttttg cgccgcccga
    14101 atcaattcgc tgatgacaaa tagcacacct gttagcgaaa tgcagacggt tattcggcgt
    14161 atcgcaccac ttgggtggca tcttgaaata tacgttcagg gcgaggagtt gccgggcctc
    14221 gagaagacga tactctcgtt acccgtgacc gtcgcgatcg accatatggg acgcattccg
    14281 acca
//
LOCUS       DATNDT010000313         7586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_599784, whole genome shotgun sequence.
ACCESSION   DATNDT010000313 DATNDT010000000
VERSION     DATNDT010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7586
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_599784"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            15..149
                     /locus_tag="VN788_10120"
     CDS             15..149
                     /locus_tag="VN788_10120"
                     /inference="COORDINATES: protein motif:HMM:NF018229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HXU20924.1"
                     /translation="MRDGKVVFMLERRDIENRDAYAIASDLTKAFDRFCAPATANTSL
                     "
     gene            complement(144..392)
                     /locus_tag="VN788_10125"
     CDS             complement(144..392)
                     /locus_tag="VN788_10125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20925.1"
                     /translation="MFQMPRRFFTPVIIGEDEVVGTPPKGSPPCLQRRFLRPQVDIDE
                     KCECATAQPLGRWLNRAEYTPAGAAMVKPSILFGGPGL"
     gene            735..2141
                     /locus_tag="VN788_10130"
     CDS             735..2141
                     /locus_tag="VN788_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HXU20926.1"
                     /translation="MLSTLCLSAGNLRAQQAGGTGPAASAADQQNQRIANLEKQVASA
                     QSAGDNSWMLTSSALVLLMTGPGLALFYGGLVRRKNVLSTMMQSFTMMAVITVLWAIV
                     GYSIAFGSGTSFIGGLQHVFLRGVGVAPDADYSATIPLQTYMVFQLMFAIITPALITG
                     AFAERMKFSAMLVFLSLWSLIVYDPMAHMVWGKGGWLNASLGGRFPTLDFAGGTVVHI
                     TSGVSALVCALYLGKRLGYPKKPMPPHSVVISFIGACLLWVGWFGFNAGSALGAGGLA
                     TSAFVATHFGAAAAAIGWGGAEWIAKGKPSALGAISGCVAGLVAITPASGFVSPMSAI
                     VIGLIAGVFCFWMVTTVKSRFGYDDSLDAFGVHGAGGTIGALLTGVFASSLINPIFKD
                     KNGNALPSGLIEGNAHQMMNQLVAVLIAWALAIVGTLVILKFVDLVIGLRATEEDESQ
                     GLDLSQHGEEGYNLESPA"
     gene            2157..2495
                     /locus_tag="VN788_10135"
     CDS             2157..2495
                     /locus_tag="VN788_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXU20927.1"
                     /translation="MTKIEAIIQTSKLDEVKGALHDIGVEGMTVIEARGHGRQKGHTE
                     VYRGREYTVDLIPKIKLEMVLADDMVERAIQAIVASARTGKIGDGKVFLTKVDDAIRI
                     RNDERGDAAL"
     gene            2601..3395
                     /locus_tag="VN788_10140"
     CDS             2601..3395
                     /locus_tag="VN788_10140"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HXU20928.1"
                     /translation="MVDIHCHILPGLDDGAESLEIAQAMGEMAIADGVTHVIGTPHAS
                     QNYRFAPGVVRERRDELQAIFAGRLILATGCDFHLSFENLQDIKHDPARFTLNQKNYL
                     LVEFADFSIPPAMDQSLHELQLAGLHPIVTHPERNPLIRAQPERLYKWLGQGCYAQIT
                     AQSLGGKFGKSAQEMAEQWLNAGAVHFIASDAHNITLRPLRLKETFDRIAAGNGEDIA
                     RALLVDNPLAAFEGRPLPYVPDLPEDLITNDSEPQRQRRRKRFWFL"
     gene            complement(3586..4479)
                     /locus_tag="VN788_10145"
     CDS             complement(3586..4479)
                     /locus_tag="VN788_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012006511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1932 domain-containing protein"
                     /protein_id="HXU20929.1"
                     /translation="MAIVGFGEAGGIFGSDLAAQGVDVSIFDILLHAKESRDAMLAKA
                     RESKVQAKESLADCLRAAELAISAVTASSSGDVARDAAKTMRPGQIFMDINSVSPAAK
                     RAEAAHIARSGAHYVEAAVMAAVPPQRLKVPMLLGGSCAADLAPRLALLGMDATAVSD
                     KIGVASAVKMCRSVMIKGMEALAVECLFAARRYGAEDAVLASLDATYPQMGWRDELPD
                     YLVSRVAEHGKRRAAEMREVAESLREVGIDPLMAEAAAERQDRLVDAMAKENLKYKNI
                     APFSWIALAEMLSAASSFKKK"
     gene            complement(4508..4945)
                     /locus_tag="VN788_10150"
     CDS             complement(4508..4945)
                     /locus_tag="VN788_10150"
                     /inference="COORDINATES: protein motif:HMM:NF024966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU20930.1"
                     /translation="MTETEILNMEQACEALAIEYAEIVDAQDYERLREIFTEDATLVR
                     RATTQETVRGLDNIIASFTSRPRNRLTYHILSNIRVHVETPDTAGGTCRVLLFTSDTS
                     EPQTPEGRKVAPKQLVGTYHDRYIRTNDVWRFTERRGDILFHT"
     gene            complement(5002..>7586)
                     /locus_tag="VN788_10155"
     CDS             complement(5002..>7586)
                     /locus_tag="VN788_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXU20931.1"
                     /translation="KNGFGISSASADARSIVYSQFGALHVYDLSTRQDKAINVAVSAD
                     MPQLLPHWEKVAKHILNSGISPSGARAVFEAHGEILTVPAEKGDARDITKSPGAADRD
                     PAWSPDGKSIAYFSDASGEYQLYIRDQKGLEPARKISLSSSPSFYYSPTWSPDSKKIA
                     YSDKFLNLWYVDVDHPTPVKVDQNLMEFNENDFGQQWSPDSQWIVYTKVLPNYLHAVF
                     VYSLATRKSTQITDGMSDCRYPGFDKDGKYIYFAASTNTGLTAAGFDMSSDDHPVTLS
                     VYVAVLRKDLPSPAPPESDEEKGPESKPSGTEPEKGKTEQKADEKKTGDQAAPKAAEK
                     PKEPPKVSIDFPDILQRILSLPIPAANYASMSVGKEGELFLVEAPIVPTGQFPPVLTV
                     SKFEMKTRKTDKLISGVTAFSLSDNGEKMLYKQGDNWFIAETAKAPEAGKGKLKTEDM
                     EVSVQPREEWNQMYHEVWRIERDFFYDPHYHGLNLPAAEKDFAVFLPGIASRGDLNYL
                     FREMLSYMSVGHMFVRGGAEPDTPHVSVGLLGADYSIENGRYRFEKVYSGENWNPKLQ
                     APLTQPGVNVKAGEYLLGVNGRDIKSSDELYTFFQETAGKQVTLKVGPNADGSGSREV
                     DVVPLASEDDIRNLDWIDSNRRKVDGLSGGKLAYVYLPDTGADGFANFNRYFFAQVGK
                     EGAIIDERFNHGGQLADYMVDYLRREPMDMVVTREGITTYDPAMAIYGPKVMIINQFA
                     GSGGDALPWLFHRQGIGTLVGVRTWGGLVGIGGYPELMDGGRVTAPRLAVAGLKGSWE
                     VENHGIAPDVEVWQDPKLVREGHDPQLERAVAEAMQQLKEHPLPKYEQPPYTSHHPEL
                     PSMP"
BASE COUNT         1555 a         2242 c         2109 g         1680 t
ORIGIN      
        1 cttccatcgc gctgatgcgc gacggaaaag tggtcttcat gctcgaacgc cgggacattg
       61 aaaaccgcga tgcttacgcc atcgccagcg acctaacgaa agctttcgat cgtttctgcg
      121 ccccagccac cgcgaacacc agcctataaa ccgggacccc caaagagaat actcggtttg
      181 accatcgccg cgcctgcagg agtatattct gcgcggttca gccacctgcc gagcggctgc
      241 gcggtagcgc actcacattt ctcatcgatg tcgacttggg gccgcaggaa cctgcgctgc
      301 aggcaaggtg gcgagccctt cggaggagta cccaccactt catcctcgcc aataatgacc
      361 ggtgtaaaga atcggcgagg catttgaaac atctatcctc tccatcaaaa tgttgcattt
      421 tactggcgtg aaacttccgt ataatctccg gccctcggga ttcaccccct taaaaaaagg
      481 agagttatat ggccgaaaaa gaagacgggt gagatgcagg catcgtccac cgacaagtac
      541 cagtttgacc attgtccgcg caggaggagt aagttcggcg cggtttgggc atcggctgcc
      601 aacaagttgc gcgacagcgc actcacattc tcaatcgaca tcgacaatcg actcgggtcg
      661 caggcacatg cgctgcggtg ccaggtgccg ggtcctggag gagtttcttg aagaaagctc
      721 tcgttctatt tttcatgctg tcgactcttt gcctatcagc cggaaatctc cgtgcgcagc
      781 aggcgggagg cacgggccca gccgcctccg ccgccgacca gcagaaccag cgcattgcca
      841 atctcgaaaa gcaagtggcc agcgcacaga gcgccggtga caattcctgg atgctcacca
      901 gttcggctct cgttctgctc atgaccggac ccggactggc gcttttctat ggcggccttg
      961 tacgcagaaa aaatgtcctg agcacgatga tgcaaagctt cacgatgatg gctgttatca
     1021 ccgttctttg ggcaatcgtg ggatacagca ttgccttcgg atcgggaacc agtttcatcg
     1081 gagggctgca gcacgtcttc ctgcgaggcg tgggcgtcgc gccggacgcc gactattcgg
     1141 cgacgatccc gttacagacg tacatggtgt tccaattgat gttcgccatc atcacgcctg
     1201 cgctcatcac cggcgccttc gcggagcgga tgaaattcag cgccatgctg gtgttcctct
     1261 ctctgtggtc gctgatcgtt tatgacccga tggcgcacat ggtttgggga aagggtgggt
     1321 ggctgaacgc gagtcttggc ggccgttttc caacactgga cttcgcgggc ggcaccgtgg
     1381 tgcatatcac gtccggcgtt tcggcgctgg tttgcgcgct gtatctggga aaacgcttgg
     1441 gatatcccaa gaagccgatg ccgccccaca gcgtggtaat cagtttcatt ggcgcgtgtc
     1501 tgctgtgggt gggctggttc ggcttcaacg ccggcagtgc actgggagcc ggcggattgg
     1561 caactagcgc atttgtggct acgcatttcg gagcggccgc cgcagctata ggctggggcg
     1621 gcgccgagtg gatcgcgaaa gggaaaccca gcgccctggg agcgatctcg gggtgcgtgg
     1681 caggtctcgt cgcaatcacg cccgcttcgg ggtttgtaag cccgatgtcc gctatcgtta
     1741 tcggactcat tgctggagtc ttctgtttct ggatggtcac aaccgtaaaa tcgcgcttcg
     1801 gatacgacga ttcgctcgac gccttcggcg tgcacggggc aggcggcacc attggcgcac
     1861 ttttgactgg cgtttttgcc tcaagcctca tcaatcccat attcaaagac aaaaacggaa
     1921 atgcgttgcc ttcgggacta atcgagggca atgcccatca gatgatgaat caattggtcg
     1981 cggttctgat cgcctgggcg ctggcaattg tcggcacgct ggtgattctg aagttcgtcg
     2041 atttggttat cggtctccgc gcgacggaag aggacgagag ccagggcctg gatctgagcc
     2101 aacacggcga ggaaggctac aatctcgaaa gcccggcata gagagcgaaa aacgatatga
     2161 caaaaatcga agcgattatc caaacgtcga aactggacga ggttaagggc gcgctgcacg
     2221 acattggcgt cgagggcatg accgtgattg aggcgcgcgg tcatggccgc caaaaaggcc
     2281 acacggaagt ctatcgcggc cgtgaatata ccgttgactt gatccccaaa atcaagcttg
     2341 aaatggtcct ggccgacgac atggtcgaaa gggccatcca agctatcgtc gcatcggcgc
     2401 gcacgggaaa aattggcgat ggcaaagtgt tcctaacgaa agtggacgac gccatccgca
     2461 tccgtaatga cgaacggggt gacgcggcgt tgtgacccag tgggtggccg tgagccagcc
     2521 gcgaatcgcc gcttgtctcg tgcgagaagg taactccttc gcattgtgat cattgtggcc
     2581 gtgcgttatt ctgaccagca atggtggata ttcactgcca catacttccg gggctcgatg
     2641 acggtgcgga atccctcgaa atcgcgcagg ccatgggcga aatggccatc gccgacggcg
     2701 tcacgcacgt tatcggcacc cctcacgcga gccaaaacta caggtttgct cctggcgtgg
     2761 tacgcgagcg ccgcgatgag cttcaggcca ttttcgcggg acgcctcatt ctagccacgg
     2821 gctgcgattt ccatctgagc tttgaaaatt tgcaggacat caagcacgat cccgcgcgct
     2881 tcacgctcaa tcaaaagaat tatttactcg tggagtttgc ggatttctca attccgccgg
     2941 caatggacca gtcgctgcac gaattgcagc ttgccggctt gcaccccatc gtcacgcatc
     3001 ccgagcgcaa tccgctgatc cgggcacaac ccgaacgtct ctacaagtgg ctcggacaag
     3061 gctgctacgc gcagatcacc gcgcaatccc tgggagggaa atttggaaaa tccgctcagg
     3121 aaatggcgga gcaatggttg aacgcgggag ccgtccactt tatcgccagc gacgcgcaca
     3181 atattacttt gcggccgctg aggctgaagg aaaccttcga tcgaatcgcc gcggggaacg
     3241 gggaagacat cgctcgcgcg ttgctggtcg ataacccgct tgccgccttc gaaggacgcc
     3301 ccctgcccta cgttcccgat ctgcccgagg acctcataac caatgattcc gaaccgcaga
     3361 gacagcgccg ccgcaaacgc ttctggttcc tctgagacga cgcccattgg aatcgcgctc
     3421 ccgcgaaggc ccaaaacacc acacccaccg tggacgattt ttctttacac caacaactac
     3481 ttgcgccgtt tcgcggcctc gtgtaaagat tttatccgta ttcttggact gcgcccctac
     3541 cgcgtcagct tgcagcaaac tactagaccg acaaatgcga ggagtctatt tcttcttgaa
     3601 ggacgaggct gcgctcagca tttctgcgag tgcgatccag gaaaacggcg cgatgttctt
     3661 gtacttgaga ttctctttgg ccattgcgtc cactagccga tcttggcgct cggccgccgc
     3721 ttcggccatc agggggtcaa tcccaacttc gcgcagagat tccgccacct cgcgcatctc
     3781 ggccgcgcgg cgctttccat gctcggctac gcggctgaca agataatccg gcaactcgtc
     3841 gcgccatccc atctgaggat aggtggcatc gagcgaagcc agcaccgcgt cttccgcacc
     3901 gtaacggcgc gcggcaaaaa gacactccac cgcgagcgct tccattccct tgatcatcac
     3961 gctgcggcac atcttcacgg ccgacgccac gccgattttg tcgctcacag ccgtggcatc
     4021 catccccagg agcgcgagac gcggggcaag gtcggcagcg cagcttccgc cgagcagcat
     4081 cggtactttc aagcgctgcg gaggtactgc cgccatcacc gcagcctcga cgtaatgcgc
     4141 accgctccgt gcaatgtgcg cggcttcggc gcgcttcgcc gcgggcgata cggaattaat
     4201 gtccatgaaa atttggccgg gacgcatggt cttcgcggcg tctcgcgcca cgtcgcccga
     4261 agaagacgcc gtgaccgcgg aaatcgccaa ttccgcggcg cgcaaacaat cggcgaggct
     4321 ttcttttgct tgcactttgc tttcgcgagc cttcgccagc atcgcgtcgc gagattcttt
     4381 agcgtgcagc agaatgtcga aaattgaaac gtcgacgccc tgcgccgcaa gatcgcttcc
     4441 gaaaatgccg cccgcttcgc cgaatccgac aatcgccacg cgccgaattg gctcgggaac
     4501 cataagccta ggtgtgaaat aaaatatcgc cgcgacgttc cgtaaatcgc cagacgtcgt
     4561 tcgtgcggat atagcgatcg tgatacgtgc ccaccaactg cttcggcgca acttttcgtc
     4621 cttcgggcgt ttgcggctcc gaagtatcgg aagtgaaaag cagcacgcga caagttccgc
     4681 cggcggtgtc cggcgtttcc acgtgcacgc gtatatttga caagatgtga taggtaagcc
     4741 ggttgcgcgg ccgcgacgtg aaagaagcga tgatattgtc caaaccgcga accgtttcct
     4801 gggttgttgc ccgccgaaca agggtcgcat cctccgtgaa gatttcacga aggcgctcgt
     4861 agtcttgcgc atccacaatt tcagcgtatt caatggcgag cgcttcgcac gcttgctcca
     4921 tatttagaat ttcggtttca gtcaacggat gtctctcctt cttgcggata ttcgatggcg
     4981 ggttttcgct cgtcagaccc gctagggcat ggatggcaac tccggatgat gcgaagtgta
     5041 gggtggctgc tcgtatttcg gcagtggatg ttccttcaat tgttgcatgg cctctgcgac
     5101 ggcccgctcc aattgcggat cgtggccctc ccggaccagt ttcggatcct gccagacctc
     5161 gacatcaggc gcaatcccgt gattttccac ttcccaagac cccttcagtc cagcaaccgc
     5221 gagccgcggg gcagtcactc ggccgccatc catcagttcc ggatagcctc caatcccaac
     5281 cagaccgccc caggttcgaa ctccgaccag cgtgccgatc ccttgtctat ggaaaagcca
     5341 cggcagggca tcgccaccgg aaccggcaaa ctggttgatg atcatcacct tcggtccgta
     5401 gatcgccatg gccggatcgt aggtggtaat tccctcgcgg gtgaccacca tatccatcgg
     5461 ctcgcggcgc agataatcga ccatgtagtc cgcaagttgt ccgccatggt tgaaccgctc
     5521 gtcgataatt gcgccttctt tgccgacctg cgcgaaaaag tagcgattga aatttgcgaa
     5581 gccgtcggcg cccgtatccg gcaagtacac gtacgcgagt ttgccgccgc tcaatccatc
     5641 cactttgcgt cgattgctat cgatccaatc cagattgcga atgtcgtcct cgctggccaa
     5701 aggcaccacg tccacttcgc gagatccgct tccatccgca ttgggaccga ccttgagcgt
     5761 gacttgcttg ccggcggttt cctgaaaaaa agtgtagagc tcgtcggaag atttaatgtc
     5821 gcgtccgttc accccgagca gatactcgcc ggccttcaca tttacccccg gctgcgtgag
     5881 cggtgcttgc agcttgggat tccaattttc tccgctatag accttttcga agcggtagcg
     5941 cccgttttca atcgaatagt ccgcgccgag caatccgaca ctgacgtgtg gagtgtccgg
     6001 ttcggcgccg cctcgcacga acatgtgacc caccgacatg taactgagca tttcgcgaaa
     6061 caggtaattg aggtctccgc ggctggcgat tcccggcaga aagaccgcaa aatccttttc
     6121 cgccgctggc aaattgagcc cgtgatagtg cggatcgtag aaaaagtcgc gctcgatccg
     6181 ccacacttcg tgatacatct ggttccattc ctcgcgcggc tggacggata cttccatgtc
     6241 ctccgtcttc aattttcctt ttccagcctc cggcgctttg gctgtttcgg cgatgaacca
     6301 gttgtcgccc tgtttgtaga gcatcttctc gccgttatct gagagcgaaa aggccgtaac
     6361 accgcttatg agcttgtcgg tcttgcgggt tttcatttca aactttgaaa cggtcaagac
     6421 cggcgggaat tgcccggttg gaacaatagg cgcttccacc aggaagagct cgccttcctt
     6481 gcctaccgac atgctcgcgt aattcgcggc cggtatcggc agcgataaaa tccgctgcag
     6541 gatgtccggg aaatctatgc tgaccttggg cggttccttc ggtttttcgg ccgctttagg
     6601 cgcggcttgg tcaccggttt tcttttcgtc ggctttttgt tctgtttttc ccttttccgg
     6661 ttccgtcccc gaaggcttgc tttccggtcc cttctcttca tcactctcgg gcggagctgg
     6721 cgacggcaaa tcctttcgca gcacggcaac gtacacgctc agcgtcaccg gatggtcgtc
     6781 gctcgacata tcgaatcccg ccgcagttaa gcccgtgttg gtgctggcgg cgaaatagat
     6841 gtacttccca tccttgtcaa aaccggggta gcggcaatcg ctcattccgt ccgtgatctg
     6901 cgtgctctta cgcgtggcaa gtgagtagac gaacaccgca tgcaagtaat tgggaagcac
     6961 tttggtgtag acgatccatt ggctgtcggg cgaccactgt tgaccaaaat cgttttcgtt
     7021 aaactccatc agattttgat ccacttttac cggcgtagga tggtccacat ccacgtacca
     7081 gagattcagg aatttgtccg aataggcaat tttcttgctg tctggagacc acgtcggcga
     7141 gtaatagaaa gatggcgagc tgctgaggct gatcttccga gccggctcga ggcctttctg
     7201 atctcgtatg taaagttgat attctcccga cgcatccgaa aagtacgcga tcgatttccc
     7261 gtccggcgac caagcaggat cgcggtccgc ggccccggga ctcttggtga tgtctcgcgc
     7321 gtctcccttc tccgcaggca ccgtaaggat ttcgccgtgc gcttcgaaaa cagcgcgagc
     7381 gccgctcgga gagatcccgg aattcagaat gtgcttcgcc accttttccc aatgcgggag
     7441 cagttgcggc atgtcggccg aaacggctac gttgatcgcc ttatcctgac gcgtcgaaag
     7501 atcgtaaacg tggagtgcgc cgaattgcga atacacgatt gatcgagcgt ccgctgaagc
     7561 ggaactgata ccgaatccgt tcttgt
//
LOCUS       DATNDT010000314         4000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1599672, whole genome shotgun sequence.
ACCESSION   DATNDT010000314 DATNDT010000000
VERSION     DATNDT010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4000
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1599672"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..1562)
                     /locus_tag="VN788_10160"
     CDS             complement(213..1562)
                     /locus_tag="VN788_10160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXU20932.1"
                     /translation="MDGKSLSKQICATLRAKGFQAYLVGGCVRDILLGREPADYDVST
                     DATPDVVQQIFPRSLAVGAQFGVIIVLDDSAPENPAQVEVATFRSDIGYTDGRHPDRV
                     AYAKSPEEDVRRRDFTINGLLLDPETEKVLDFVGGRKDIEAGVIRAIGDPIQRFTEDK
                     LRMLRAVRFSARFNYVLESNCVKAVDALGAQIAQVSAERLRDELTKILTEGAAHRGFE
                     LFDSTGLLKYLLPEVSRMKGVAQPPQYHPEGDVWIHTLLMLEKLPAGCPPALAWGVLL
                     HDVGKPPTFAPPEITGDRIRFNEHAEVGTRMAEAICRRFRFSNEETDQIAALVANHMK
                     FKDVLNMKPATLKRFVRQQRFEEHLELHRLDCLSSHGNLENYRFTKHFIETTPPEQVR
                     PPRLLTGDDLKSMGFAPGPAFREILEAIENAQLDGKITDHKAAIQMVQDDFANLRSN"
     gene            complement(1575..2750)
                     /locus_tag="VN788_10165"
     CDS             complement(1575..2750)
                     /locus_tag="VN788_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXU20933.1"
                     /translation="MAVRETATITAIRDRGRRVRGFWESVSGGFELEQLWDQFLSEAR
                     ASYGLFSHDVDWDGIDREPKRWKKPFRVMWGLFQAMLMKLSPARRVLLLLAIIVILIP
                     TDIHVGGGLTRISVSWFGSVLLFLILTLELADRVTLKRDLEIAREIQQWLVPAKAPEI
                     SGLDIAFATRPQNTVAGDCYDAFLRPANAAGVQPLVIAVADVAGKSVPAALLMATFQA
                     SLRALAGTSATLEENVIGLDRYSRAHNLEGRRFTTAFIAEIDPVARVMRYVNAGHNAP
                     LLVHASRSAERLEDGGPPLGLPLFHGAEPHYECGSIQLAAGDLLFIFTDGVIDAVNED
                     DDEFGEQRLLSLLSSLPNISAADMRDRVMGELNEFVGTARQQDDITCLLLRVTENRA"
     gene            complement(2924..>4000)
                     /locus_tag="VN788_10170"
     CDS             complement(2924..>4000)
                     /locus_tag="VN788_10170"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXU20934.1"
                     /translation="GGPDQRGVVTSASYEARRFGVHSAMPLRTAAKLCPQGVFLENRH
                     HLYSEWSDRVAKILGKYSPIVEMASIDEAYVNLHGTERLFGPPLAASHKLLKDITAST
                     GLPCSGGLGSTRLIAKVGSDQAKPRGLVWVPAGSEENFLAPLSVRRIPGIGKVTEAAL
                     NKLGIETVAQLQTQTLARLEADFGRWGTALYRKARGVDSFEFFYDAEPKSISHNQTFG
                     EDTRDRPQLESTLSYLCQKATKRLRDAALHARTVTLTLRFSDFKTITRNHTLSEPSDL
                     DTVILKALRDLFVASWDGKRLVRLVGAALSSFSSASGQLDLLDPGRREKLERLARATD
                     GLRDRFGFNKLQLGGSLAHKPTHD"
BASE COUNT          828 a         1058 c         1282 g          832 t
ORIGIN      
        1 caagttgtcc cccttggaca acgaagattc aacgaccgac aacagggaaa tcgaccgtct
       61 aaagcgttag atgcaggaaa aggggaaagg acttttctat tcctttcgac acttataaaa
      121 tatgagcata caaggctaaa ctttatgatt caagccctat ttactcccat tttaagaacg
      181 aaacatcttg caaaacattg caagtaagca ccttaattcg acctgagatt cgcaaaatcg
      241 tcctggacca tctggatcgc agccttatga tccgttattt tcccatcaag ttgcgcattt
      301 tctatcgctt ccaaaatctc ccggaaggcc ggtccgggcg caaagcccat ggatttcagg
      361 tcgtcgccgg ttaagaggcg cggtggacga acctgctccg gtggcgtcgt ttcgatgaag
      421 tgttttgtga aacgatagtt ctccaggttg ccgtggcttg acaggcaatc gagccggtgc
      481 agctccagat gttcctcgaa gcgctgctgg cggacgaacc tttttagcgt ggccggcttc
      541 atgttcaata catctttgaa cttcatgtgg ttggcgacaa gggccgcgat ttggtccgtt
      601 tcctcatttg agaatctgaa gcgacggcag atcgcttcgg ccatgcgggt gccaacctcg
      661 gcgtgttcgt taaacctaat ccggtcacca gttatctctg gcggcgcgaa ggttggaggc
      721 ttgccaacat cgtggaggag aacgccccag gcaagggccg gagggcagcc tgccgggagc
      781 ttttccagca tgaggagcgt gtggatccag acgtcgccct ccgggtgata ttggggcggc
      841 tgagcaactc ctttcattct ggagacttcc gggagcaaat atttgagcag gccggttgaa
      901 tcgaacaact cgaaacctcg gtgggcggcg ccttcggtga ggattttggt gagctcatcg
      961 cggagacgct cggcggagac ttgggcaatt tgtgctccga gtgcgtcaac cgccttgacg
     1021 caatttgact ctaaaacgta gttaaaacgc gctgaaaagc gcacagcgcg cagcatacgg
     1081 agcttgtctt cggtgaaccg ctgaattggg tcgccgatgg cccggatcac gcccgcttcg
     1141 atgtcttttc gtccgccgac gaagtctagg accttttccg tttcgggatc gaggagcagg
     1201 ccgttgatgg tgaagtcgcg gcggcggacg tcttcctcgg gcgatttcgc gtaggcgacg
     1261 cggtcgggat ggcggccgtc ggtatagccg atatcggagc ggaaggtggc gacttcgacc
     1321 tgcgcagggt tttccggcgc ggaatcgtcg agaacaatga tgacgccgaa ctgggcgcct
     1381 acggcgaggc tgcgcgggaa gatctgttga acgacgtccg gcgtggcgtc tgtggaaacg
     1441 tcgtaatcgg ctggctcgcg gccgagaagg atgtcacgca cacagccgcc gaccagatag
     1501 gcctggaatc ctttggcccg caatgtggcg cagatttgct tcgaaagcga tttgccatcc
     1561 atatgtcgat gccgctaggc tcgattttcc gttacgcgca gcagaaggca agtgatgtcg
     1621 tcctgctggc gcgctgttcc gacaaattcg ttcagctcgc ccattacgcg atcgcgcata
     1681 tcggcggcgc tgatattggg caggctgctt aagagcgaaa gcaatcgctg ctcgccgaac
     1741 tcgtcgtcgt cctcgtttac ggcgtcaatc acgccatccg tgaagataaa gagtagatcg
     1801 cccgctgcca gttggatgct gccgcactcg tagtgaggtt cagcgccgtg aaagagcggc
     1861 aggccaagag gaggcccgcc gtcttcgagc cgctcagcgg agcgagatgc atgaaccagc
     1921 agcggagcat tatgaccggc gtttacatag cgcatgacgc gggcaaccgg atcgatctcc
     1981 gcgatgaacg ccgtggtgaa gcggcggccc tcgagattgt gggcgcggga gtagcgatcg
     2041 agaccgatga cgttttcttc gagggtggcg gacgtgccgg cgagagcgcg caagctggct
     2101 tgaaacgtgg ccatgagcaa ggcggcggga acgctctttc ctgctacgtc cgcgacggcg
     2161 attacgagcg gctgcacgcc ggccgcattc gcggggcgga ggaatgcgtc atagcaatcg
     2221 cccgcgacgg tattttgcgg tcgcgtggcg aaggctatat ccaagcccga aatttcaggc
     2281 gctttcgcgg gcacgagcca ttgctggatt tcacgcgcga tttcaagatc gcgttttaag
     2341 gtgacgcggt cggccaattc gagagtcaga atcaaaaaga gcaggaccga gccgaaccac
     2401 gagacgctga tgcgagtgag tccgccgccg acgtgaatgt ccgtgggaat cagaatgacg
     2461 atgattgcga ggagcagcag gacccggcga gcggggctga gcttcatcag catcgcctgg
     2521 aacagacccc acatcacgcg gaacggcttt ttccagcgct tcggttcgcg atcgatgcca
     2581 tcccaatcga catcatgcga gaaaaggccg tagctggccc gggcttcgga gaggaattga
     2641 tcccaaagct gttcgagttc aaagccgccg ctgacacttt cccagaagcc gcgcacacgg
     2701 cgaccacgat cacggattgc ggtaattgtg gctgtttcac gcacggccat ggagcgatta
     2761 tagagtgatg gcgagacaaa cggtggagtt tactgggcgt gtgctgcgcg ggcggtcgca
     2821 atcgttcact atcaaagggc gaaaccaaga gaggtccttc cgctcgtgag ttcggtgtca
     2881 agcgtcagga tgacgacatt ttgcttcggc gcaggggctc gaattaatcg tgggtgggtt
     2941 tgtgggcgag ggagccgccg agctggagtt tgttgaagcc gaagcgatcg cggagaccgt
     3001 ctgtggcgcg ggcgaggcgc tcgagttttt cgcggcggcc gggatcgagc aggtcgagct
     3061 gcccggatgc ggatgaaaac gagctgagcg cggcgccgac gaggcgcacg aggcgttttc
     3121 cgtcccacga ggcgacgaaa agatcgcgca gcgctttcag gatcacggta tcgagatcgg
     3181 atggttccga gagtgtgtga ttgcgcgtga tcgtcttgaa atcgctgaag cgcagcgtaa
     3241 gcgtgacggt tcgggcatgc aaggccgcgt cgcgaaggcg cttggtagcc ttttgacaaa
     3301 gatagctgag cgtggattcg agttgcgggc ggtcgcgcgt gtcttcgccg aacgtctgat
     3361 tgtgcgagat ggatttgggc tcggcgtcat aaaagaattc aaaggaatcg acgccgcgcg
     3421 ctttgcgata cagcgcggtg ccccagcggc caaaatcggc ttccaggcga gcgagagttt
     3481 gcgtttgcaa ttgcgcgacg gtttcgatgc ccagcttatt gagcgcggct tcggtgactt
     3541 tgccgattcc cggaatgcgg cggacggaaa gcggcgcgag gaaattttcc tcgctgcctg
     3601 cgggaaccca aaccaggccg cgcggcttgg cttggtcact tccgactttt gcgatgaggc
     3661 gagttgagcc gagtccgccg gagcacggca ggccggtgct cgctgtgatg tccttgagca
     3721 acttgtgcga agcggcgagc ggcggaccga aaaggcgttc ggtgccgtgg agattcacgt
     3781 aggcttcatc aatcgaagcc atttccacaa tgggcgaata ttttccgagg atttttgcga
     3841 cgcgatcgct ccactcacta tagagatggt gccgattctc gaggaaaacg ccttgcgggc
     3901 acaattttgc ggctgttcgc aacggcatcg cggaatgcac gccgaagcgg cgcgcttcat
     3961 agctggccga cgtgacgacg ccgcgctgat caggcccgcc
//
LOCUS       DATNDT010000315         3888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3700910, whole genome shotgun sequence.
ACCESSION   DATNDT010000315 DATNDT010000000
VERSION     DATNDT010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3700910"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            354..1838
                     /locus_tag="VN788_10175"
     CDS             354..1838
                     /locus_tag="VN788_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU20935.1"
                     /translation="MINKLAKLFASVAAPIAITVVCYVFLRVNATTVAMLYLLAVLLI
                     AAFSGLLESTIAAVTAVVCLNFFFLPPVLTFTIADPQNWLALFAFLATAIVGSQLSSY
                     ARKQTTEAWNSKHETEQLYSLSRTILLLDEHSSAASQIAGHIVRIFGFKSAVIFERYS
                     ASLHGGDEVSAHLTNQLKEVANKGTLIKDEERQLTIASIRLGVEPIGSLATEGSFLSD
                     GAQQALLNLIAIALERERHREQTTQAQAERKSERLKSTLLDAIAHEFKTPLTSIKAAS
                     GSILSSSLLRTREGGDLTAARELITVIEEETDRLTDLVNDAIQMARIEAGALNPKKRP
                     CKIEDLVLQVAQDLERRRDGRSIDLQLSDNLPTFAADPDLLSLTLRLLLDNAFKYSPP
                     LSSITVGAVQQNGKIVIRVHDEGAGIPKAEQARIFEKFYRGPASENQVPGAGMGLAIA
                     REIALAHGGEIWIESESGEGTTFVLTIPAEDAPREDASAADIGS"
     gene            1810..2508
                     /locus_tag="VN788_10180"
     CDS             1810..2508
                     /locus_tag="VN788_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU20936.1"
                     /translation="MRVQRILVVDDDPQIRRVMRTALTGEGFEVADARTGEDGLERLR
                     GERFDLVLLDVNMPGIGGMETCRLMRATSEIAIVMLTVRDSEADKIGALDAGADDYVT
                     KPFSTPELLARIRAALRRVPGGTESSMGTVTLPDLEINLSARRVVVHGNHVRLTPKEF
                     DLLQYFLSNVNVPLSHGRLLQAVWGPDYGSEVEYLRVFVNQLRKKIEPNPSKPKYILT
                     EPWHGYRFQMPADA"
     gene            2570..3094
                     /locus_tag="VN788_10185"
     CDS             2570..3094
                     /locus_tag="VN788_10185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20937.1"
                     /translation="MAMVSRVGFERNDSTGPEMLGGESAVDRPPEATVLFTSKDATQI
                     AIAQAARWIKRNGASLRILVMQVVPFPEMLDHPTRLDPDIRCAKELAEESTIGEVTVA
                     VYLCRKVIEGVKCAIDRQTPVFMGAKGHSWWTGEWRLARKLQNCGYLVCCVDVQKRRA
                     RLRNISPCSIFSTF"
     gene            3245..>3888
                     /locus_tag="VN788_10190"
     CDS             3245..>3888
                     /locus_tag="VN788_10190"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HXU20938.1"
                     /translation="MTPNGLFQILIYFVVILLLTKPMGVFMVRLFEGKRTFLHPVLRP
                     LETLTYRLAGVREDVEQRWTQYTFALIAFSIFCFLFVYLLQRLQGVLPFNPQGFNASN
                     VSPDLAFNTAVSFVTNTNWQAYSGESTLSYFVQMAALTVQNFASAAVGIAGAIALVRG
                     FSRQQTNTIGNFWVDLTRAVVYILLPISLMAALFFCSQGVIQNFDHYTHFKTLEG"
BASE COUNT          825 a         1058 c         1138 g          867 t
ORIGIN      
        1 cctgcgcggg aacgccgcgc caatcccctc tgggatagat gtaatctccg gcgtcagcca
       61 aaactcgctg cccgcccagc caggatcgtt cttaaggtgg aattcgattc ccaggcggcc
      121 ggcccgcttt ccgaagtccg gcggctcatc gaatacgaga gcactccgta gatgtcgctt
      181 accgccagca gcagcgccag tccgctgaac gttccgagca caacggtatt gaaacgctgc
      241 ggcgcaacac ctggttaagc gagcacacgt cggcgatcgg catatacgca acaagcaacc
      301 acgagcataa gcgggctttc ggctaaaatg aggcggacgt aacgatgccg tcgatgatca
      361 ataaactcgc gaaattgttt gcgagcgttg ccgcgcctat cgcgatcact gtcgtttgtt
      421 atgtatttct gagagtcaac gcaaccacgg ttgcgatgct ttatctcctg gcggtgctgc
      481 tgattgcggc attttcaggc ttactggaat cgacgatcgc cgccgttacc gcggtggttt
      541 gcttgaactt cttctttctg ccgccggtac tgacatttac catcgcagac ccgcaaaact
      601 ggcttgcact cttcgcgttt ctagcgacag cgatcgtcgg gagccagctt tcttcttatg
      661 cgagaaagca aaccaccgag gcctggaaca gcaagcatga gacagaacaa ctttactcgc
      721 tgagccggac cattctgctg ctagacgagc acagcagcgc ggccagccag attgcggggc
      781 atattgtacg gatttttgga tttaaaagcg ccgttatctt cgagcgctat tccgcgtccc
      841 ttcatggcgg tgatgaagtt tcagctcatc tcacaaatca gttaaaggag gttgccaaca
      901 agggcactct catcaaggat gaggagcgac agctaacgat cgcgtccatc cgtctcggcg
      961 tagagcccat tggaagcctc gcaacggagg gatcgttcct ctccgatggg gcgcagcagg
     1021 cgctcttgaa cctgatcgcg atcgccttgg agcgcgagcg ccaccgggag caaacgacgc
     1081 aggcgcaggc ggagcgcaag agcgaacggc tcaaatcgac actcctcgac gccattgctc
     1141 acgagtttaa gacgccgctc acgtcgatca aagccgcgtc cggttcgatc ctgtcgtctt
     1201 ccctgctccg tacgcgcgag ggaggagacc tcacggcggc tcgcgaactg atcaccgtga
     1261 ttgaagagga gactgaccgg ctcacggatc tagtcaatga cgcgattcaa atggcccgga
     1321 ttgaggcggg tgcgctcaat cccaaaaagc ggccgtgcaa gatagaggat ttagtgttac
     1381 aggtggcgca ggatctcgag cgtcgccgcg acgggcggtc catcgatttg cagctcagtg
     1441 acaatctgcc tacctttgcc gccgaccccg atctgctcag ccttacgcta aggctgttac
     1501 tggacaatgc gttcaagtac tcgccgccat taagttctat caccgtcggc gccgttcaac
     1561 aaaatgggaa aattgtgatt cgcgtccatg acgagggcgc gggcattccc aaggcagaac
     1621 aagcgcgcat tttcgaaaag ttctatcgcg gacctgccag tgagaaccaa gttccgggcg
     1681 ccggcatggg cttggcgatc gcgcgggaaa ttgcgctcgc gcacggcgga gagatctgga
     1741 ttgaaagtga aagcggcgag ggcactacgt tcgtgttgac gattccggcc gaagacgcgc
     1801 cgcgagagga tgcgagtgca gcggatattg gtagttgacg acgatccgca gatacgccgg
     1861 gtgatgcgca ccgcgctgac cggggagggt ttcgaagtcg cggatgcccg taccggagag
     1921 gacggactgg agcggttacg cggcgagcga ttcgatctcg tgttgctgga cgtgaacatg
     1981 ccaggcatcg ggggaatgga gacctgccgg ctgatgagag ccacttctga aatcgccatc
     2041 gtcatgctga cggtgcgaga ctccgaagcg gataagatcg gtgcgctcga cgcgggcgct
     2101 gacgattacg tgacgaagcc gttcagcacg cctgagctgc ttgcgcgaat ccgtgccgcg
     2161 cttcggcggg ttcctggtgg tactgaaagc tcaatgggca cagtcactct gccggatctc
     2221 gagattaacc tgagcgcccg gcgcgtcgtg gttcatggga accacgtgcg gctcactccc
     2281 aaagaattcg atctgcttca gtattttctt tcgaacgtca acgtgccgct gtctcacggc
     2341 cggctgctac aagccgtctg gggtcctgat tatggcagtg aagtcgagta tctgcgcgta
     2401 tttgtgaacc agttgcgaaa gaagatcgag cccaatccgt cgaagccgaa atatattttg
     2461 accgaaccct ggcacggata tcgttttcag atgcccgcgg acgcttagga aatccttacg
     2521 gaattcttat gcgtttttta cggcggtcct cgtattctcg ggatggaaca tggcgatggt
     2581 ctcccgagtc ggcttcgagc ggaatgacag caccgggcca gagatgttgg gcggcgaatc
     2641 cgcagtcgat cgtccgccgg aggcaacggt cctcttcacg tccaaagatg caactcagat
     2701 cgccatcgcg caagcggccc gctggataaa gagaaacgga gcaagtcttc ggattctcgt
     2761 catgcaagtg gttccttttc cggagatgtt ggatcacccg acccgcctgg atcccgacat
     2821 acgttgtgcg aaggagctgg cggaagaatc cacgattggc gaggtcacgg ttgcggtgta
     2881 tctatgcagg aaagtgatcg aaggcgttaa atgcgccatc gaccgtcaga caccggtctt
     2941 tatgggtgca aagggccatt cctggtggac cggggaatgg cgcctggcgc gaaagctcca
     3001 gaattgcggc tacctggttt gctgcgtaga cgttcaaaaa cgcagagcga gattgaggaa
     3061 tatctctcca tgctcgattt tttctacatt ctgatcgcga ttcttttctt tgtcgcgtgt
     3121 tgggcgttca cgaaggcgtg cgaccgcctc taaggaacta tatggattac atcattggcg
     3181 gcatcgcgac ggtatttctg ttcggatacc tgatttacgc gcttctcaag ccggagaaat
     3241 tttgatgaca ccaaacgggt tgtttcagat actgatctac ttcgtcgtca ttctcctatt
     3301 gacgaaaccc atgggtgttt tcatggtgcg gctcttcgag gggaagcgga cgtttctaca
     3361 tccggttctg cgcccgctgg agacgctgac ctaccggctg gcgggcgttc gggaagatgt
     3421 ggagcagcga tggacacaat acacgttcgc gctgattgca ttcagcatct tctgcttcct
     3481 gttcgtttac ctcctgcaaa gacttcaggg agttctgccg ttcaacccgc agggattcaa
     3541 cgcttccaat gtctcgccgg atctcgcttt caatacagcg gtgagtttcg tcaccaatac
     3601 aaactggcag gcgtacagcg gcgagtcgac gttgagttac ttcgtgcaga tggcggcgct
     3661 aacggttcag aacttcgcgt cggctgccgt tggtattgcg ggggcgatcg cgctggttcg
     3721 cggattttcg cggcagcaga cgaatacaat cggcaatttt tgggttgatt tgacgcgagc
     3781 ggtcgtctac atactgcttc cgatctcgct gatggccgcg ctgtttttct gctctcaggg
     3841 cgtgatccaa aatttcgacc attatacgca ctttaagacg ctcgaagg
//
LOCUS       DATNDT010000316         6429 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3019140, whole genome shotgun sequence.
ACCESSION   DATNDT010000316 DATNDT010000000
VERSION     DATNDT010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3019140"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..224
                     /locus_tag="VN788_10195"
     CDS             <1..224
                     /locus_tag="VN788_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20939.1"
                     /translation="SKHLRLLRRAHLVQEHRQGRQRLYQLNPGPLRAVDSWLSQYRCF
                     WQMSLTSLKTLIEAGATKRTSRRTRKKKK"
     gene            240..731
                     /locus_tag="VN788_10200"
     CDS             240..731
                     /locus_tag="VN788_10200"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXU20940.1"
                     /translation="MATSKVTPDGDAIVTEVHIDAAPDRVFQALVDPQQVPQWWGQGG
                     VYRCTKFHADLRVGGKWLCEGIGPDGGDFKIVGEYQEIDPPHLLVYTWVASWTGDMKT
                     TVRWELEPTNPGTLLRIRHSGLAAYPQAAQSYRGWPRMLGWLRAFLDNGETVADRKQA
                     AAK"
     gene            809..1159
                     /locus_tag="VN788_10205"
     CDS             809..1159
                     /locus_tag="VN788_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016876141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXU20941.1"
                     /translation="MIQDHLSTTFAALADPTRRAILARLISDEASVTELARPFEMSLP
                     AVSKHLKVLERAGLIERSREAQWRPCRLRAKPLKEISDWVEHYRRFWEESFDRLDIYL
                     SELKKKERKYARKK"
     gene            1217..1699
                     /locus_tag="VN788_10210"
     CDS             1217..1699
                     /locus_tag="VN788_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016551128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXU20942.1"
                     /translation="MSSVASSKEREVTITRIFDAPRELVFKAWTDPKHLAKWFGPKGF
                     TNPVCEADARVGGTWHIVMRSPEGTEHPCGGVYREVVEPERLVFTNIATDKEGKPILD
                     GLTSVVFEKHGDKTKLTMHTRATAVVDYAVERLKGMEAGWTQTLERLGEELARLQSAR
                     "
     gene            1735..1797
                     /locus_tag="VN788_10215"
     CDS             1735..1797
                     /locus_tag="VN788_10215"
                     /inference="COORDINATES: protein motif:HMM:NF018663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20943.1"
                     /translation="MRDKFGVSWQIIPIALGQTL"
     gene            1978..2382
                     /locus_tag="VN788_10220"
     CDS             1978..2382
                     /locus_tag="VN788_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXU20944.1"
                     /translation="METTTQEAPVSKGRLWTGYIISGLVVLFMIFDGVTKVMKVPQVI
                     QASAKIGYNATTVVGIGATLLVCTALYVIPRTSIFGAILLTGYLGGAVDANVHSGTAL
                     FNITFPMAFGVLAWLGIYLRDRRLRALVPLRS"
     gene            2521..3549
                     /locus_tag="VN788_10225"
     CDS             2521..3549
                     /locus_tag="VN788_10225"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU20945.1"
                     /translation="MIIDSHQHFVRLARHPEVEGFRQAQLSGKNPDAIPSISDADIKK
                     SLEQVISLMDSRGIDVAFASPRAKAMATHEGTPEQNAVWADLNNGLILRACQLNSGRF
                     APVGMLGQHPSCPSEQVAKQIERWTNEGFIAFNFDPDGTGGRWQNQPFTGKHNYAVFE
                     AAQEVDVPLVLHGSESINKNFPHTAVQYLVADITGFVQLMMAKDLFRDFPRLRFLIPH
                     AGGALPCQIGRWRAIAKHNSWASPDELLGNGNVFVDTCVYNRLNMQMLFDAVPLDNIL
                     FGSECDGAFPGVVNDETHDHYDNTRRYVDDSKLKAGDKARVYVANARRVYPHASRWVQ
                     AERSLGLA"
     gene            3733..4410
                     /locus_tag="VN788_10230"
     CDS             3733..4410
                     /locus_tag="VN788_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010337692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HXU20946.1"
                     /translation="MSKPVVVKRVEQPDQDAVAALEKYGVSTVHEAQGRIGLLAAYMR
                     PIYPGASIAGSAVTVSLPPGDNLMIHVAVEMCTPGSILVVAPTSPSTDGYFGELLGTS
                     LRAHGAKGLVIEAGVRDVHPLSEMKFPVWSRAVHSQGTVKATLGSVNVPIVCAGAAID
                     PGDVIVADDDGVVVVKREDAAKVAAASKQRVDKEDAVRERLAKGELGLDIYGLRAKLK
                     EMGLEYR"
     gene            4490..>6429
                     /locus_tag="VN788_10235"
     CDS             4490..>6429
                     /locus_tag="VN788_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease"
                     /protein_id="HXU20947.1"
                     /translation="MTKSVPAKLFLSSVLFVVFACCSFGDAKTPLLLQTPTISKDQIA
                     FVYGGDIWIVGKDGGQAHRLVTGRDLESGPIFSPDGSRVAFSGNYDSNVDVYVVPSTG
                     GEPRRLTYHPGDDIAIGWTPDGKNVLFHSHRNSTNDPDKLFTVPASGGFPTELPLDMA
                     EEGSYSPDGSQIAYVPVFQWEPDWKGYRGGQTTPVWIAKLADSSVVKVPREDSNDRNP
                     MWIGKTVYFLSDRDGPTTLFAYDTETQKVSRVVDNKNGFGISSASADARSIVYSQFGA
                     LHVYDLSTRQDKAINVTVSADMPQLLPHWEKVAKHILNSGVSPSGARAVFEAHGEILT
                     VPAEKGDVRDITNSPGAADRDPAWSPDGKSIAYFSDVSGEYQLYVRDQKGLEPARKIS
                     LSSSPSFYYSPTWSPDSKKIAYSDKFLNLWYVDVDHPTPVKVDQNLMEFNENDFGQQW
                     SPDSQWIVYTKVLPNYLHAVFVYSVATRKGTQITDGMSDCRYPGFDKDGKYIYFAAST
                     NTGLTAAGFDMSSDDHPVTLSVYVAVLRKDLPSPAPPESDEEKGPESKPSGTEPEKGK
                     TEQKVDEKKTGDEAAAKAAEKPKEPPKVSIDFPDILQRILSLPIPAANYASMSVGKEG
                     ELFLVEAPIVPTGQFPPPLTVS"
BASE COUNT         1464 a         1717 c         1897 g         1351 t
ORIGIN      
        1 tttcgaagca cctgcgtctc ttgcggcgcg cgcatctcgt tcaagagcat cgtcagggcc
       61 ggcaacgtct ctaccagtta aatcctggac cgctgcgggc ggtcgattcc tggctcagtc
      121 aatatcgatg tttctggcag atgagtctga ccagcctaaa gactcttatt gaagccgggg
      181 ccacgaaaag gacatcgcgc cgtactcgaa agaagaaaaa atagctagga ggactcaaca
      241 tggcgacatc gaaagtcacg cccgatgggg acgcaattgt caccgaggtg cacatcgacg
      301 ccgcacctga tcgcgtcttt caggcgcttg tggatcctca gcaagtacca caatggtggg
      361 gacagggcgg ggtgtatcgc tgcacgaagt ttcatgccga tctacgagtc ggtgggaagt
      421 ggctctgtga agggatcggc cctgacggag gcgacttcaa aatcgtcggc gaatatcagg
      481 aaattgatcc gccgcatctg ctcgtctaca cgtgggtagc aagctggacc ggcgacatga
      541 aaacgacagt gcgctgggag ctggagccaa caaatccggg cacgcttctc agaatccgac
      601 acagcgggct cgcggcatat cctcaggcgg cgcaaagtta tagaggctgg cccagaatgc
      661 tcggatggct gcgggccttc ttggataatg gggaaacggt agcggatcgc aaacaggccg
      721 cagcaaagta atcaaacttg ccaatcgtag aaaaggcttg acatggtaat cgcgggcatt
      781 gtatttaacc cataggttaa atacaattat gattcaagac cacctcagca ccacatttgc
      841 cgcgctcgcc gatccgaccc gtcgcgcgat cctagctcgg ctgatttcag acgaggcatc
      901 tgttaccgaa ctggcgcggc cgtttgagat gagcctgccc gccgtgtcca aacacttaaa
      961 agtgttggag cgcgccgggc tgatcgagcg cagccgcgag gcgcagtggc ggccatgccg
     1021 cctgcgggcc aagccgctca aggagatttc ggactgggtg gaacactatc gtcgcttctg
     1081 ggaagaaagt ttcgatcgcc ttgatattta tctaagcgaa ttaaagaaaa aggagaggaa
     1141 atatgctcgc aaaaagtaat cccgctggga acgccgaggt catcgatcgg ctcgcacgac
     1201 tcgtcggagg aatttcatga gcagcgttgc atcgtccaaa gagagggaag tcaccatcac
     1261 gcgcatcttc gatgctccgc gggagcttgt gttcaaagca tggacagacc cgaaacacct
     1321 cgcgaaatgg tttggaccga agggatttac gaatccggtt tgtgaagcag acgcgcgtgt
     1381 tggaggcacc tggcatatcg tgatgcgttc gccggagggt accgagcatc cgtgcggcgg
     1441 cgtgtatcgc gaagtcgtcg agcccgagcg gctcgttttc accaatatcg ccaccgacaa
     1501 agaggggaag ccgattctcg atgggcttac aagtgtcgtc ttcgagaaac acggggataa
     1561 aacgaaactc actatgcata cccgcgctac agcggttgtc gactacgcag tcgagcgcct
     1621 gaaaggcatg gaagcgggtt ggacgcaaac cctggaacgt ctgggcgagg agctagcgag
     1681 attacaaagt gctcgctagt ttgggaaaga ttccgtccga accagtgcgg ctggatgcga
     1741 gacaaattcg gcgtttcgtg gcagattatt ccgattgctc tgggccagac gctttgagac
     1801 cgcatcctgc taaatcggcc agggtcatga aggctatgct tcaagtgcgc aagatcgaca
     1861 tgcaaggctt gaagcaatct aacgaccacg agtagctcga cacccgaaag tgaatgccgt
     1921 gcggcaggac ggtttttctt gttcgattat cgagtgcaaa aacggaggaa atgacaaatg
     1981 gaaacgacta cccaagaggc tcctgtctcg aaaggaaggc tttggaccgg atatatcatc
     2041 agcggcctgg ttgtattgtt tatgattttt gacggcgtga cgaaagtgat gaaagtgccg
     2101 caggtgattc aggcctcagc caaaattgga tacaacgcca ccacggttgt gggaataggc
     2161 gccacgctgc ttgtttgcac ggcgctctat gtgattccgc gcacgtccat ttttggcgcg
     2221 attctgttga cgggctatct tggcggcgca gttgacgcca acgtgcacag cggcacagcg
     2281 ctttttaata ttacgttccc catggctttc ggtgtgctgg cctggctggg aatttatttg
     2341 cgggatcgtc gcctgcgcgc attagttccg ctgcgaagtt agcgcgcttg gcgttgcgcc
     2401 gatgctccac gatgctccgc ggattgacgc acgctttgtg ccccactaga atcccaactc
     2461 ggagatacaa aaggcggacg ggcgcatatc tttggagaaa ggtttcaact agccgcgaat
     2521 atgatcatcg actctcacca gcacttcgtt cgtcttgcgc gtcacccgga agtggagggc
     2581 tttcgccaag cgcaactttc cggaaagaat cccgatgcca ttcccagcat ttccgatgcg
     2641 gacattaaaa agagcctgga gcaggtcatc agcctgatgg actcgcgtgg catcgatgtc
     2701 gcattcgctt ctccccgtgc gaaagccatg gcgacacatg aaggtactcc ggaacagaac
     2761 gccgtctggg ccgatctcaa taacggcctt atccttcgcg cctgccagtt gaattccggc
     2821 cgctttgcgc cggtgggcat gctcgggcaa catccttcgt gtccatccga gcaagtggcg
     2881 aaacagatcg agcgttggac gaatgagggt ttcatcgcgt tcaatttcga tcccgatggt
     2941 accggcggcc gctggcagaa tcagcccttt accggaaagc acaattacgc ggttttcgaa
     3001 gcggcgcagg aggtggatgt gccgctcgtg ttgcacggca gcgaatcgat caacaagaac
     3061 ttcccgcata cggccgtcca atatctggtc gccgatatta cgggatttgt gcaactcatg
     3121 atggcgaaag atctgttccg ggattttccg cgtctgcgtt ttctgatccc gcacgcgggc
     3181 ggcgcgttgc cctgccagat cgggcgctgg agggcaatcg cgaagcacaa cagttgggcc
     3241 agcccggacg aactcctcgg caacggcaac gttttcgtcg atacctgcgt atacaatcgc
     3301 ctgaacatgc aaatgttatt tgacgccgtc ccgctcgaca acatcctgtt cggctcggaa
     3361 tgcgatggtg cgttccccgg cgtggttaat gacgagacgc acgatcatta tgacaatacg
     3421 cggcgctatg tcgatgattc gaaactgaaa gccggcgaca aggccagggt atatgtcgcc
     3481 aacgctcggc gcgtttatcc gcatgcgagc cgttgggttc aagcggaacg gagtctgggt
     3541 ctggcgtaga atccaagttt caggcgcccc tgctgggcag cgattgacgc cgcgcttttg
     3601 caccgtctaa aatcccaatc cagtccctga gaaagctatt tttgtggctg atagaaagga
     3661 aacagtgatc gagaaaatcc agcataagca agcgtatcag cgcatcttct cgaatatgaa
     3721 cgaggagcgg tcatgagcaa accggttgtg gtgaagcgcg tggagcagcc cgatcaagac
     3781 gccgtggccg cactcgaaaa atatggcgtg tccacggttc acgaagcgca gggacgcatc
     3841 ggattgctcg cggcttacat gcgtccaatt tacccaggtg cgtcgattgc cggcagcgcg
     3901 gtcaccgttt cgttgcctcc gggcgacaat ttgatgatcc acgtggcggt ggaaatgtgc
     3961 acgccgggca gcatcctggt agtggctccc acatcgccca gcaccgacgg ttattttggc
     4021 gagctgcttg gcacctcgct tcgcgctcac ggcgcgaagg gtttggtgat cgaagccggg
     4081 gtccgcgatg ttcatccgct ttcggaaatg aagtttccgg tctggagccg cgcagtgcat
     4141 tcacaaggca cggtgaaagc tacgctcggc tcggtgaacg ttccgattgt atgcgcgggg
     4201 gccgcgatcg atccaggcga cgtgattgtc gccgacgatg acggcgtcgt ggtggtgaaa
     4261 cgagaggacg cggcgaaagt ggctgcggca tcgaagcagc gcgtggacaa ggaagatgcg
     4321 gtacgcgaac gtctggcgaa aggcgagttg ggcctggata tttatggcct gcgcgcgaaa
     4381 ctgaaagaaa tggggctcga atatcgttaa gtataaagac ggccgatttt cgaggctact
     4441 gggaaaatga tcgcctccac ctgggccgcc agcatcccgg gaggtcactg tgactaagtc
     4501 cgttcctgcc aaactatttc tctcttctgt gctgtttgtt gttttcgcct gctgctcctt
     4561 tggggacgcg aaaactccgt tgctgctcca gacccccacg atcagcaagg accagattgc
     4621 atttgtgtat ggcggagaca tctggatcgt cggcaaagat ggaggccagg cgcaccgtct
     4681 cgtcactggg cgcgacttgg aaagcgggcc tatcttttcg ccggatggat cgagggtcgc
     4741 gtttagcggc aactacgaca gcaatgtcga tgtgtacgtg gtgccgtcaa ccggtggcga
     4801 acctcgccgg cttacctatc atcccggtga tgatatcgcc atcggatgga cgccggatgg
     4861 caagaacgtc ctcttccatt cgcatcgcaa cagcacgaac gatccggata agctcttcac
     4921 cgttccggca tcaggcggat tccctacgga gcttccgctc gacatggccg aagaaggttc
     4981 gtactcgccg gatggctcgc aaatcgcgta cgttcccgtc tttcaatggg agccggactg
     5041 gaagggctac cgaggtgggc aaaccacgcc ggtctggatc gcgaagctag ccgattcgag
     5101 cgtggtcaaa gtgccgcgcg aggattccaa cgatcgcaat ccgatgtgga tcggtaagac
     5161 cgtgtatttt ctctcggacc gcgacggtcc gactactctc ttcgcttatg acacggagac
     5221 tcaaaaagtc tcgcgcgtag tcgacaacaa gaacggattc ggtatcagtt ccgcttcagc
     5281 ggacgctcga tcaatcgtgt attcgcaatt cggcgcgctg catgtttacg atctttcgac
     5341 gcgccaggat aaggcgatca acgtaaccgt ttcggccgat atgccgcaac tgcttccgca
     5401 ttgggaaaag gtggcaaagc acattctgaa ttctggcgtc tctccgagcg gcgctcgcgc
     5461 tgttttcgaa gcccacggcg aaatcctcac agtgcccgcg gagaagggcg acgtgcggga
     5521 catcaccaat agtcccgggg ccgcggaccg cgatcctgct tggtcgccgg atgggaaatc
     5581 gatcgcgtac ttttcggatg tgtcgggaga atatcaactt tacgtacgag atcagaaggg
     5641 cctcgagccg gctcggaaga tcagcctcag tagctcgcca tctttctatt actcgccgac
     5701 ttggtctccg gatagcaaga agatcgccta ttcggacaaa tttctgaatc tctggtacgt
     5761 ggatgtggac catcctacgc cggtaaaagt ggatcaaaat ctgatggagt ttaacgaaaa
     5821 cgattttggg caacagtggt cgcccgacag ccaatggatc gtctacacca aagtgcttcc
     5881 caattacttg catgcggtgt tcgtctactc agttgccacg cgtaagggca cgcagatcac
     5941 ggacggaatg agcgattgcc gctaccccgg ttttgacaag gacgggaagt acatttattt
     6001 cgcggcgagc acaaacacgg gcttaactgc tgcgggattc gacatgtcga gcgacgacca
     6061 tcccgttacg ctgagcgtgt acgttgccgt gctgcggaag gatttgccgt cgccagcccc
     6121 gcccgagagt gatgaagaga agggaccgga aagcaagcct tcggggacgg agccggaaaa
     6181 gggaaaaaca gaacaaaaag tcgacgaaaa gaaaaccggt gacgaagccg cggccaaagc
     6241 ggccgaaaaa ccgaaggaac cgcccaaggt cagcatcgat tttccggaca tactgcagcg
     6301 gattctatcg ctgcctatac cggcggcgaa ttacgcgagc atgtcggtag gcaaggaagg
     6361 cgagcttttc ctggtggaag cgcctattgt tccgactggg caattcccgc cgcccttgac
     6421 cgtttcgaa
//
LOCUS       DATNDT010000317         9256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4091369, whole genome shotgun sequence.
ACCESSION   DATNDT010000317 DATNDT010000000
VERSION     DATNDT010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9256
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4091369"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..773
                     /locus_tag="VN788_10240"
     CDS             <1..773
                     /locus_tag="VN788_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20948.1"
                     /translation="GLWEFDIWTDELDGQQMGPIGMQRAKIYAAQMQATFTPAWQAKL
                     AQLKKTADAAKAKDGSEPPAFEEFGQFGGCGWTPGFPATMLPGTYEWRVTPEEATLIS
                     LLGSVRHIYTDGRPHPSKDELWPMSQGDSIGHWEGDTLVVDTVSIRSPVYFPGLARGV
                     GVPFIPMSNELHTTERIRMVNHDQMEIQFTVEDPIALAKPVHVTFDWHRVKDINRMEQ
                     NADDCDSAASDRNPIVNGRYKTLVKPTPEAPAKPKGRR"
     gene            complement(789..1976)
                     /locus_tag="VN788_10245"
     CDS             complement(789..1976)
                     /locus_tag="VN788_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HXU20949.1"
                     /translation="MSCRFSVLARCALFSLISVLLSIAPAIRAQSNSGKAHGKTRTYY
                     IAADEVNWNYAPSGRDEAMGMPFDEISKLYTQSGPHRIGSVYKKAVYREYTDASFSTL
                     KPRPPDEQYLGILGPILRGEVGDTIKIVFKNNASHPYSMHPHGVVYQKDSEGADYNDA
                     TGGADKSDGGVPPGATHIYTWNLAERSGPGPNDPSSIFWLYHSHADELRDVASGLFGG
                     IIVTRRGMALPDGRPKDVDREFVNIFIAINENESWYLDDNIRDHATDPKGVNRAESRL
                     ITPTGVVGTVVGTGFVETNIKWSINGYLYGNMPLMTMKQGDHVRWYVASLGDFNNFHT
                     PHWHGNTVVVAGQRTDVLNLGAAQMVTADMVPDAPGIWLYHCHVSDHMLAGMMARYEV
                     KPR"
     gene            complement(2156..2521)
                     /locus_tag="VN788_10250"
     CDS             complement(2156..2521)
                     /locus_tag="VN788_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20950.1"
                     /translation="MSADSNAWPWPDSLDAVIAAPKHHKLMFENDRVRILEVRIPPGE
                     LVPVHTHRWPSAIYVAKQSDFLRRDGNGDLLFDSRTAGPPPIEPMAQWTPPLPPHSVE
                     NIGSTEILLITAELKEPSG"
     gene            2651..3082
                     /locus_tag="VN788_10255"
     CDS             2651..3082
                     /locus_tag="VN788_10255"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXU20951.1"
                     /translation="MRILLIMLGKTQRAESRALLDDYVSRIRHYAEVEVSELRDSSAA
                     ALRKLKIETGAAVVLLDAAGKQFTSQQFASWLGQLRDSGTREIAFLCGDADGFPPEVR
                     ASARHKVSLSTLTMPHEFARVILAEQIYRAFAILARHPYPR"
     gene            complement(3142..4443)
                     /locus_tag="VN788_10260"
     CDS             complement(3142..4443)
                     /locus_tag="VN788_10260"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HXU20952.1"
                     /translation="MTFLRRVPRRVIQLTCFVGAIVLVWPLVPWVMAPRFLQQISPFV
                     AISSSVAARSIGLGAILALVVLVASSFKKRWFCLYLCPTGFLLENTNHLGLRKASFWS
                     KCPPIGRYVAVLTLVGSVVGYPVLLWMDPLCIFSSPFAINKATNPFSVILVCLGLGVL
                     VGGTFLFGMLWCVRICPLSGLQDMLFAMRKALWTRLTAGAPSRKLAGADLVEISGVST
                     SELRSVRRESVSQRAKKFNAARRVFFAGGAGALLALWARHAGAARYRKSDTLLRPPGA
                     VPEDRFTGLCIRCNNCVHACPTKIIHPDTGHEAGLAGFGAPVVKFDRDYKYCLETCND
                     CTQVCPTGALTVLPLQQKNKYIIGEALVDNNVCLTALGKKECDACELACPYDAVKITW
                     DEAQYNAYPIVNDKCVGCGACEVVCPTTPVKAIRVWTLKPA"
     gene            complement(4440..5834)
                     /locus_tag="VN788_10265"
     CDS             complement(4440..5834)
                     /locus_tag="VN788_10265"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXU20953.1"
                     /translation="MRRSIAAGGLLAAGSLTPISSALAGDVGGQAVTHSPTGTEHRIP
                     ATLSGDSVVQPRREVPVLHKTSVLVVGGGPAGTAAAFSAKRLGVDVTLVERYGYLGGL
                     ATGGLVLGIFPLYNRNKQQVIFGIGEEMMKKLDVMKYGIIDRNKAPIYPTIDAEAFKY
                     LLADMVLDSGITTYLDCWGVDAIVDAKGAVRGAIFESKSGRQAILADIVIDCSGDGDI
                     YAAAGAAFEKVKTNIGLVSRIGNINQVDMAPGRGSEDPEHPMVGKPSSGRPSKGRVSD
                     FNGHAGNSTPAPHVNWLNMRGPVGDALDIADLTALELKHRQGTWSNVEKLRQKPGNEE
                     VYLTETAPQLGVRLSRLLDGVKKLTAQEIHSGAKFQDVIGYSGAYANAPEFQIPYGSL
                     VPAKVENVLAAGRCISAEFEVADIARLIPVCWVTGQAAGLAAALCIQNKCKPRHINVA
                     KLQNLLHQQGAYLG"
     gene            complement(6077..6436)
                     /locus_tag="VN788_10270"
     CDS             complement(6077..6436)
                     /locus_tag="VN788_10270"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXU20954.1"
                     /translation="MAKLPGDYARPAGRLALAFQGNEVAGCGAVRPLGGDVCEMKRLY
                     VRSAFRGKGVGGAVVDALISSAREIGYQGMRLDTLPSMAAAIAIYRSLGFREIAPYRA
                     NPVPGALFFELDLVKLG"
     gene            6605..7195
                     /locus_tag="VN788_10275"
     CDS             6605..7195
                     /locus_tag="VN788_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXU20955.1"
                     /translation="MKYPVEQKAETHERIIDAAARSFREHGSEGEGLAQLMKQLGLTH
                     GGFYRHFQSKEDLYVDAITRGFQQAADGIIAAAAKAPQGAQLRVIIERYLSLEHLEDP
                     GGGCVISTLAAEIARQRPAVRARINAAMKSYRERLLPFFPGKSVAEKRRQFIVLFPSM
                     AGVLMTARAMTDPDARKEILSVARRFYIRAFAEHVE"
     gene            7272..7805
                     /locus_tag="VN788_10280"
     CDS             7272..7805
                     /locus_tag="VN788_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20956.1"
                     /translation="MLPKDIFRIDMVLGYLAWALCIATYVWPRLRSMDRVEAHRAIAT
                     FNSFRFFGLVFLLPGFVGLSLPQAFAAPAAYWDLATAVLAILALLTVRVRSLFWPLVW
                     AFNLVGLADLIMDTMRAVRVDLPSVAGQLGAGYAIPMLYVPALFWVHLLAFWLLLRPA
                     HARVPSTHALVSPGATE"
     gene            8058..>9256
                     /locus_tag="VN788_10285"
     CDS             8058..>9256
                     /locus_tag="VN788_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20957.1"
                     /translation="MPSRHRVPLWLMGLTNLSYGLYGGVIAFALPQLLGGRNVPETAI
                     AGLTAVAFSPSFYAFLVSPILDVRFSRRWYAVVLAIVASFTLVVAFLNLDHLVLLETV
                     LTVGFFAAYLCQSAIGGWLSSITSGEEEKSVSVWMTIGNVGGFGVMAISCNQLARLLS
                     PLALALVLGASVLLPVIVFPFMQAPGPDRRLASESFPRFFGDLVSLVKQREVLVAIIL
                     FAAPAATFSLTNFLGGRGNDFHASPGFVGLVGGVGVAAGGIVGCLVFPLLSRIMPLRP
                     FYLAVGIVGGLCTLALIVLPRTPASFATLLITENLFQSLAITTSTAVIFETIGRDNPL
                     ASTTFCFVGSAYGVPISYMLYVDAFGFARGGITGSLAIDAITGIFASVLLGLMLLSLS
                     RRPKPLPA"
BASE COUNT         1864 a         2676 c         2697 g         2019 t
ORIGIN      
        1 ctggattgtg ggaatttgat atctggactg acgaactgga cgggcagcaa atgggcccga
       61 taggaatgca gagagcaaaa atctatgccg cacagatgca ggcgacgttt actcccgcat
      121 ggcaagcgaa gctcgcgcaa ctaaagaaga cggcagatgc ggccaaagcc aaagacggta
      181 gcgaaccgcc cgcattcgag gaattcggac aattcggagg gtgcggctgg acgcccgggt
      241 ttccggccac aatgctgccc gggacatatg agtggcgtgt gactcctgaa gaagccacgc
      301 tgatcagcct tctcggttcg gtgcgacaca tctacacgga cgggcggccc catccgtcga
      361 aggacgagct gtggcccatg agccagggcg attcgattgg acactgggag ggggatacgt
      421 tggtggtcga cactgtgtcg ataaggtcgc cggtgtattt tccgggattg gcgagaggcg
      481 tcggggttcc gttcattccg atgagcaatg aattgcatac caccgagcgg attcgcatgg
      541 tgaaccacga tcagatggag attcagttca cggtggaaga tccgatcgcg ctggccaagc
      601 ccgttcacgt gactttcgat tggcatcgcg tgaaagacat caatcgcatg gaacaaaacg
      661 cggatgattg cgacagcgcg gcgagcgacc ggaatccgat tgtgaatggc agatataaga
      721 ctcttgtaaa accaactccc gaggcgcccg ccaaaccaaa gggtcgccgc taacgccttg
      781 acgaatttct agcgcggctt cacttcgtag cgcgccatca ttcctgcaag catgtggtcg
      841 ctgacgtggc agtgatagag ccagatgccg ggtgcatcgg gcaccatatc ggcggtgacc
      901 atttgcgcgg cgcctaagtt aaggacgtcg gtgcgctggc ccgcaacaac aacggtgttg
      961 ccgtgccaat gcggcgtgtg aaagttattg aaatccccga gactcgccac gtaccaacgc
     1021 acgtggtcgc cttgcttcat agtcatcagc ggcatgtttc catagaggta gccgttgatg
     1081 gaccatttga tgttcgtttc cacaaagcct gtacccacga ccgtgcctac cactcccgta
     1141 ggggtgatca gccgcgattc cgcgcggttc acgcccttgg ggtcggtggc gtggtcgcga
     1201 atgttgtcgt cgagatacca gctctcgttt tcattgatgg cgatgaaaat gttgacgaac
     1261 tcgcggtcca cgtcctttgg gcggccgtcg gggagcgcca ttccgcggcg cgtgacgata
     1321 attccgccga acagcccgga agcgacgtcg cggagttcgt ccgcgtgcga gtgatagagc
     1381 cagaaaatgg agctgggatc gttcggaccg ggacctgagc gctccgcgag gttccatgtg
     1441 taaatatggg tggcgccggg aggcactccg ccatcgcttt tgtcggcacc gccggtggca
     1501 tcgttgtaat ccgcgccttc ggaatctttt tgatagacga ctccgtgggg atgcatgctg
     1561 taagggtgcg aggcattgtt cttgaagacg attttgatcg tatcgccaac ttcgccgcgc
     1621 aaaatggggc cgaggatgcc gagatactgt tcgtcgggcg gccgaggttt aagcgtggaa
     1681 aaagaggcgt ccgtatattc gcgatagacg gcctttttgt acacgctgcc gatgcggtgg
     1741 ggtccggatt gagtgtagag tttggagatt tcgtcgaatg gcattcccat cgcttcatcg
     1801 cggcccgacg gcgcataatt ccaattcact tcgtccgcgg caatgtagta ggtgcgcgtt
     1861 ttgccgtggg ccttgccgga attgctttgc gcgcgaatgg cgggcgcgat tgaaagcagc
     1921 accgaaatca gagaaaaaag agcgcaacgg gccaagaccg aaaatcgaca agacatgaat
     1981 tccctccggg aatcgattcg caggaccggc cgagttggca gcgtcgaaac ccagcggtgc
     2041 gcgaagcggc tgatgaaggt gaattgatat ttccaatttg gccggtaaaa ctggcgagat
     2101 tctacactgg ggatacgaac gcgagaagaa atgctttgct tggtgagcgg tggagttagc
     2161 ccgaaggttc ctttaattcg gcggtgatca ggaggatttc agttgagccg atattttcga
     2221 cggagtgcgg cggcagcgga ggcgtccatt gcgccatcgg ctcgatcggc ggaggaccgg
     2281 cggtgcggct gtcgaaaagc aaatctccgt tgccgtcgcg gcggagaaaa tcgctctgct
     2341 tcgccacgta aatggcgctg ggccagcgat gcgtgtggac gggcacaagt tcgccgggcg
     2401 ggatgcgcac ttcgagaatg cggacgcggt cgttttcaaa cattaacttg tggtgctttg
     2461 gcgcggcgat taccgcgtcg agcgaatctg gccagggcca tgcgttcgag tctgcactca
     2521 cgctgattct ccgtcgccga gcatcgcgca ttaggagagc attctgcatc gcgccccctc
     2581 gcgaagaccg gcgcagagcg atgatagtat atggcgtcag ataaattcga aaggcgcttg
     2641 caaactctcc gtgagaatcc ttttgatcat gctcgggaag acacagcgtg cagaatcgcg
     2701 ggcgctgctc gacgattatg tgagtcgcat ccggcattat gccgaagttg aagtgagtga
     2761 actgcgcgat tcttccgccg ccgctctccg caaattgaag atcgagacag gggcagccgt
     2821 ggttttgctg gatgctgccg gaaagcaatt cacttcacag caattcgcca gctggcttgg
     2881 gcaactgcgc gacagcggta cgcgcgagat tgcgtttcta tgcggcgacg ccgacggctt
     2941 cccacctgaa gttcgtgcaa gcgcgaggca caaggtttcg ctctcgacgc tgaccatgcc
     3001 tcatgagttc gcccgcgtca tcctggcgga gcagatttat cgagcgttcg cgatcctcgc
     3061 gcgccatcct tatcccaggt gagagcggca cgcggctgca gtaattcaac ttccggctgc
     3121 ttgcagacca gctggcagca gctaggcagg ctttaaggtc cacacgcgaa tggccttgac
     3181 tggcgtggtg gggcaaacga cttcgcaggc tccgcagccg acgcatttat cattgacgat
     3241 cgggtatgcg ttgtactgcg cctcgtccca tgtgattttg accgcgtcgt agggacatgc
     3301 tagctcgcac gcgtcacatt ccttcttgcc gagcgccgtg aggcagacat tgttatcgac
     3361 gagcgcttct ccgatgatgt atttgttttt ttgctggaga gggagcaccg ttaacgcgcc
     3421 ggtggggcaa acctgcgtac agtcattgca cgtttccagg caatacttgt agtccctgtc
     3481 gaatttgacg actggcgccc cgaagcctgc gagacccgct tcatgacctg tatcagggtg
     3541 aatgattttc gttgggcagg cgtgcacgca attgttgcag cggatgcata gcccggtaaa
     3601 acgatcctcg ggtacggcgc cgggcggacg cagcagcgtg tcacttttgc ggtatcgcgc
     3661 tgctcctgcg tgccgcgccc aaagcgcgag caatgcgccg gcgccgccgg cgaagaacac
     3721 acggcgcgcg gcgttgaact tcttcgcgcg ttgacttaca gattcacggc gaactgagcg
     3781 gagctcagag gttgaaacgc cgctgatttc aaccagatca gcgccagcga gttttcgact
     3841 tggcgcaccc gcggtgagcc gggtccaaag cgcctttcgc atcgcaaaca gcatgtcctg
     3901 caatccgctg agagggcaga tgcggacgca ccagagcatg ccaaaaagaa aagttccgcc
     3961 caccagaaca ccgaggccga ggcacaccag gatgaccgag aacgggttgg tcgctttgtt
     4021 tatggcgaag gggctgctga aaatgcacag cggatccatc cacagcaaca ctggataccc
     4081 aactacggag ccgaccaacg tgagaaccgc gacatatctt ccaatgggcg gacacttgct
     4141 ccaaaagctc gctttgcgca aacccaggtg gttggtattt tcgagcagga aacctgtggg
     4201 gcagaggtac aggcagaacc accgcttctt aaatgaactc gcgacgagca cgacgagtgc
     4261 gagaatcgct cccagaccga tggatcgcgc ggcgacactc gacgagatag caacgaaggg
     4321 actgatttgt tgaagaaatc ttggcgccat cacccagggg accagcggcc aaaccaggac
     4381 gatcgcgccg acaaagcaag tcagctgaat cacccgccgc ggcacgcgcc tgaggaacgt
     4441 catcccaaat acgccccctg ttgatgcaga agattctgaa gcttcgctac gtttatgtgg
     4501 cgaggcttac acttattttg gatgcacaaa gcggcagcca agcctgcagc ctgtccggtg
     4561 acccagcaaa ccggaatgag ccgggctata tccgcaactt cgaattcggc ggagatgcat
     4621 cgtccggccg cgagcacatt ttcgaccttc gcgggaacga gggagccgta gggaatctgg
     4681 aattcgggcg cgtttgcata ggcgccggaa tagccaatca catcttgaaa tttggcgccg
     4741 ctgtggattt cctgagccgt gagttttttg acgccatcaa ggagcctgct gagccgcaca
     4801 cccaactgag gggctgtttc ggtcaaatag acttcctcgt ttccgggctt ttggcggagc
     4861 ttctcgacat tgctccaagt cccttggcga tgcttaagtt ccagagcggt gaggtccgcg
     4921 atgtccagag cgtcgccaac aggacctcgc atattgagcc agttcacatg cggcgcgggc
     4981 gtggaattcc cagcgtgacc attgaaatcg ctgaccctac ccttggaggg gcggccgctg
     5041 gagggcttgc ctaccatggg atgctccggg tcttcggaac ctcgtcccgg ggccatatcg
     5101 acctggttga tgttcccgat tcgactgacg agaccgatgt tcgtcttaac tttttcaaaa
     5161 gccgcgcccg cggccgcgta aatatcaccg tcccccgaac aatcgatcac gatgtcggcg
     5221 aggattgcct gtcgccccga cttgctttcg aaaatggcgc cgcgcacggc tcccttggca
     5281 tctacaatgg cgtcgacgcc ccagcaatcc aggtaagtgg tgattccgga atcgagcacc
     5341 atgtcggcca ataaatattt gaaggcctcc gcatcaattg tcggataaat aggcgctttg
     5401 ttccgatcga tgattccgta tttcatcacg tccaatttct tcatcatctc ttcgccgatg
     5461 ccgaagatga cctgctgctt attcctgttg taaagcggaa aaattcccaa gacgaggccg
     5521 cctgtagcga gccctccgag atatccgtat ctttccacca gcgtgacgtc gacgcccaga
     5581 cgcttggccg aaaaggcggc cgccgtgccc gccggaccac ccccgacaac caggacgctg
     5641 gttttgtgga ggaccggcac ttcccttcgc ggctgcacga cgctgtcgcc agagagagtc
     5701 gctggaatcc tatgctcggt tccagtgggt gagtgagtca ccgcttgtcc accgacgtcg
     5761 ccggctagtg ctgacgaaat tggtgtcagc gatccagccg caagcaatcc acctgcggca
     5821 atcgaacgac gcattaattc tcttcggctc aagtcttttg gcatgggtcc acctatttgg
     5881 cagtcacgat cgcgaaccca attccgggtt cttgatctat gcgcgtcaat actatcccag
     5941 cgcgaacgag cagccaactc ccgattagtt ggctacggac agttagatga agttagggat
     6001 gcgcgccccc gcagccataa tagaaggatt gggacaatga accgaaccaa ctcggcgtca
     6061 ttctagtgac tgtgcctcag ccaagcttca cgagatccaa ctcgaaaaat aacgccccgg
     6121 gcacagggtt cgctcggtac ggcgcgattt ccctgaatcc cagcgagcgg taaatcgcaa
     6181 ttgcagcggc catcgatggc agcgtatcca gccgcatccc ctgatagccg atctcccgtg
     6241 ccgagctgat cagcgcgtcg accactgcgc ctcctacgcc cttcccgcga aacgctgaac
     6301 gaacatagag tcgcttcatc tcgcaaacgt cgcccccgag tggacgcaca gcgccgcagc
     6361 cagcgacttc gtttccttga aacgcgagcg cgagtcgtcc ggcaggcctc gcgtagtcgc
     6421 cgggcagttt ggccaactct tctacaaatc cctggacgca aaggtcgact tccagcgatt
     6481 ccgaatactc gacaaaaagc tttcgaatcg tggcgaggaa aaggagcgat ctgccatagg
     6541 gcgctcaagc cacactcgaa acttctgatt gcttttgtaa gatgatgtag gtaatcttta
     6601 agcgatgaaa taccccgttg agcaaaaggc cgaaacacat gaacggatta tcgatgccgc
     6661 cgcgcgctcg ttccgcgagc atggctctga gggggagggc cttgcgcagt tgatgaaaca
     6721 gctagggctg acgcacggcg gtttctatcg ccatttccaa agcaaggaag acctctatgt
     6781 ggatgcgatc actaggggct tccagcaggc ggcggacgga ataatagcgg cggcagcaaa
     6841 ggctcctcaa ggcgcgcaac tgcgcgtgat catcgagcgt tatctcagct tggaacatct
     6901 cgaagatccc ggcggcgggt gcgtgatttc cacgctcgct gccgaaatag cccggcaacg
     6961 tcctgcggtc cgggccagaa ttaacgccgc catgaagtcg tacagagaac ggttgctgcc
     7021 ttttttcccc ggcaagagcg ttgccgaaaa gaggcgccag ttcatagtcc ttttcccatc
     7081 gatggccgga gttctgatga ccgcgcgtgc catgacggac cccgacgcgc gcaaagagat
     7141 cctttccgtc gccaggcgtt tctacataag ggcttttgcg gagcacgtag agtaacattt
     7201 tttttgcgac tttagattac tgtcatcatc tatgagggtg acggaattcg taggtcacaa
     7261 ggaggttggg tatgctgcca aaggatatct tcagaatcga tatggtgctg ggctatctcg
     7321 cctgggcgct ctgtatcgcc acttacgtct ggcctcggct gaggtcgatg gatcgcgtcg
     7381 aggcgcaccg cgctatcgcc acgtttaata gtttccgctt tttcggcctg gtcttcttgc
     7441 taccggggtt tgtcggactg agcctgccgc aagccttcgc cgcgcccgca gcttactggg
     7501 acctcgccac cgcggtactc gccatcctcg cgctgctgac ggtacgcgtc cgctccctct
     7561 tctggccgct agtctgggca ttcaatctgg ttggcctcgc tgacctcatt atggacacga
     7621 tgcgcgcggt aagggtggac ctgccgtccg tggccggcca gctcggcgca ggttacgcga
     7681 tccctatgct ttacgtaccg gcccttttct gggtgcatct cctcgcgttc tggctgctgt
     7741 tgcgacctgc gcacgcgcgg gttccatcga cccatgcgtt ggtctcgccc ggcgcgacgg
     7801 aataaccagg aaaacggcgg acgctaagca cccgcgcgcc tggaacctct ctcataaatc
     7861 cgctccggac ggtttgcacc ccctcgcaaa ataactcgcg atttctccgt cacgcgtaca
     7921 gagcaaggcg aagtgcggcc ggagatcact tcctcaagag tgcagttgct ggtattgttc
     7981 ccgtcgcgct cttaacggat ttggccgagt acgaatagca cttcgatcga atcgattgca
     8041 ccggagccgg gcaaaaaatg ccgagtcgcc atcgggttcc tctttggctc atgggtctca
     8101 ccaacctgtc gtacggcctt tacggcggag tgattgcatt tgcgcttccg cagttgctgg
     8161 gcggtcgcaa cgttcctgag acagcaatcg ccggtctgac cgctgtcgcg ttttcgccca
     8221 gcttctacgc ttttctcgtc agccccatcc tcgatgtgcg attcagtcgt cgctggtatg
     8281 cggtcgtgct ggcgatcgtg gcctcgttca cacttgttgt agcttttctg aacctcgatc
     8341 atctcgtgct tctggaaacc gtgctcaccg tcgggttttt cgccgcctat ctttgtcaaa
     8401 gtgccattgg tggatggctc tcgtccatca cgtccggcga agaggagaag tccgtcagcg
     8461 tctggatgac gatcggtaat gttggcggct tcggcgtcat ggccattagc tgcaatcagc
     8521 tcgctcgtct cctttcgccg cttgctctcg cgttggtgct tggggcatcc gtcctgctgc
     8581 cggtgattgt tttcccgttc atgcaggcgc ctggccctga ccgcaggctc gccagcgagt
     8641 ctttccctcg attcttcggc gaccttgtct cgctggtgaa gcagcgcgaa gtgctcgtcg
     8701 ccattattct tttcgccgct cccgccgcca ctttctcgct cacgaacttc ctcggtggtc
     8761 gcggcaacga cttccacgct tctccaggat tcgttggctt ggtcggaggc gtcggcgtgg
     8821 ctgctggagg gatcgtcggc tgtctcgtct ttccgctgct cagccgcatc atgccgctgc
     8881 ggccctttta tcttgctgtg gggattgttg gcggcctgtg tacgctagcg ctcatcgtgc
     8941 tgccgcgcac tccggcgtca ttcgccacac ttttgattac agagaatctc ttccaatcct
     9001 tggccattac caccagcacc gcggtcattt tcgaaaccat cggccgcgac aacccgctgg
     9061 cttccaccac cttctgcttc gtcggctcgg cctatggcgt tccaatttcc tacatgctct
     9121 acgttgacgc tttcggcttc gcccgcgggg gaatcaccgg cagcctcgcc atcgatgcca
     9181 tcacaggcat cttcgccagc gtcctgcttg gcctcatgct gctctcgctc tcccgcaggc
     9241 ccaaaccact gccagc
//
LOCUS       DATNDT010000318         7826 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3330831, whole genome shotgun sequence.
ACCESSION   DATNDT010000318 DATNDT010000000
VERSION     DATNDT010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7826
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3330831"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..2163)
                     /locus_tag="VN788_10290"
     CDS             complement(28..2163)
                     /locus_tag="VN788_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXU20958.1"
                     /translation="MFKAYQLTAALILSLLAASAATAQAPHAFPFRATNYDVEVIVNP
                     NDQTIQAQAKVDFVADQVAKTLLVELHPDLRVTSVKSADGHALNFGRDNNSPLLLTVE
                     LQGAATPGKQITLTFEYNGPVSSADDSPTKGVRFASVDKTSAYLLLPARWFPLTDFPS
                     NRYTGTFNVIVPSTFTVAGTGKADPPVSHPGIGVGAQNQSSYVFHCDQAGPVGSFVAG
                     NLQLSPVSAQGDQFSFYTPPAQASTAAAYGNSLAQIMGYFSDTFGAFATQPSITVAEM
                     PDGSLPGYSAPGLLLISAREWTPRVNDRLLSQLAAGQWWGYQVLPATASDVWITDGLS
                     RYAEAMYAEESGGVAALNKALEDFAVGALMFEQDTPIANASRIGAYSDQYHSIVADKG
                     AMVFHMLRAQLGDDAFTALLRDFYKQHAGKTATIDEFEKLTATKVPAPKPGDPHLNLV
                     SFFSQWLNSTGIPEFSLQYIVYRTPKGFEVIGKIHQELDTFRMPVEVKVDTEGNPEYK
                     KVLVTGTTSEFKLDTFGRPKPNGITIDPNNNLLKSSPQLRIRATIARGEALALVGKYY
                     EAIQEYQHALDIQPTDSLAHFRIGEAMFYQKNYQAAANAFRSALGGDLNPKWVEVWAH
                     IYIGKIYDVLGQRERAVNEYNLAQHTKDDTAGAQEEAARYMRMAYNPESTAPTPTATG
                     SGNAAQAPVAPGDSAADKQPVLKRRPENN"
     gene            complement(2252..3391)
                     /gene="lpxB"
                     /locus_tag="VN788_10295"
     CDS             complement(2252..3391)
                     /gene="lpxB"
                     /locus_tag="VN788_10295"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HXU20959.1"
                     /translation="MTPPRILISAGEASSDMYAARLATALRQRTGAQLFGMGGPRMAE
                     AGVELIADYHEVAVVGISEVLHKIPSVVRVQRKLAAEASRRRASLAILVDSPGTHLGV
                     ARRVKNAGIPIGYFIGPQVWAWRAGRVRVVRRLVNRMVVIFPFEEKIYRDAGVPVDFV
                     GHPLADVVRPTMNREEFAARCELDPERPIVTLLPGSRRNEIARNYPPILEACQLLARD
                     MKPLGSLQFIHAVAPGLDEGLFSAYDNYPGINLKRIKGAAYDALAAADCAIVASGTAT
                     IEAALLGTPMVVIYRVAKTTAAILRRMIHTPFFSMVNLVAGRRVVPELIQDDFTPRAV
                     AAEVRYLLESLETRDEMKTGLAEVRAKLGSGGAIERAADIFARML"
     gene            complement(3422..5302)
                     /locus_tag="VN788_10300"
     CDS             complement(3422..5302)
                     /locus_tag="VN788_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU20960.1"
                     /translation="MSDFREEEKLGKIYDTQITRRLMKYMWPYRWQVALALSMTFAVA
                     AMGIVGPYLFHIAVDHYIDPGFRHSIARSAALIGVMWMVAAYLGTLLASFGFQYVQVR
                     IMQSVGQETMYDLRKEIFQHLQRLPMSFFDRSPVGRLVTRCTTDVDALNDLFASGVVA
                     MLNDFVQLFGMAIFLLWWHPMLALATLSPLPLMILLTYVFRNHVRGANRGIRTSIARI
                     NGFLNEHISGMAVVQLFNREQKARKQFANLNRAYMEAYKDAINAFSYFFPAVEFLSMG
                     GIALLFWVAGVRVIGGTIAVGVLISFMIYAQSFFRPIQDLSEKFNILQAAMAASERIF
                     QLLDEPLSVVSAAHTHSLPAPRGEIEFRNVWFAYRGGPAPKEEDWVLRDVSFRVESGQ
                     SLAIVGHTGAGKTTIIQLLLRFYDIQRGQILLDGVDIREIDLQQLRLMFGIVLQDPFL
                     FTGTLESNVRLGTETIERPVTERALREVGLGPLLESLPQGVETPVTERGGTLSVGQRQ
                     LVSFARALAHDPKFLILDEATSSVDTKTELLIREAVDRLLTGRTAIVIAHRLSTIQHA
                     NRILVFHKGRLREQGSHQELLAVHGIYYRLYQLQYKEQELSAPLAGAALSGFSQPLPA
                     DD"
     gene            complement(5299..7275)
                     /locus_tag="VN788_10305"
     CDS             complement(5299..7275)
                     /locus_tag="VN788_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU20961.1"
                     /translation="MSAVTQSIPGAAARPERRPSGWDHLKVLLPYVGRYKKMVSVGLL
                     ALAITGIVGALPQLIIGMIVDCLQGSPRALSTLSGTARHLLHPLFAIYAPLSHHALGL
                     FCIILIVVMLVKGFFSFWTRWILIGISREMEYDLRSDLLDRFLALDPEFYARNRTGDL
                     MSRATNDLNAVRMVLGPGIMYSATTIATMVLAIFFMVKLSPMLTLWVLIPVPFVALSV
                     RYFGQIIHRLSEQIQAALGVLSTRAQENLTGIRVIRAYAQEKQEIALFDKANRHYVER
                     NIKLIGSWSLFFPALSALIGLTVVILLGLGGMEVTDHKVSLGTFMSFYGFLIQLIFPM
                     IAIGFVTNIFQRGAASMGRLNYLLRANPNIRNNALSQYESAVAAADGNGQRDSRPDQV
                     KVQVNADPTHQIRGEIEFRHLNFAYPSGSDAAPNPAVLHDINLRIPAASTLAIVGPTG
                     SGKSTLAALVARLWEAPANTLFLDGRSIRDYPLDSLRRAIGYVPQDTFLFSETLSGNI
                     AFGVDESGEEEILEAAEIAGISGEIQSFPQRFETMVGERGITLSGGQKQRTSLARAIL
                     RRPRILVLDDSLSSVDTDTEERILRRLREVMRQRTTILIAHRISTVKDADQIIVLREG
                     RIIERGSHEQLLGLGGYYADLYHKQLLEEELERE"
     gene            complement(7398..>7826)
                     /locus_tag="VN788_10310"
     CDS             complement(7398..>7826)
                     /locus_tag="VN788_10310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20962.1"
                     /translation="FEIGGYWDLATFDAGNGKSKIEPQALEIFCRGEDYDDELWRQSG
                     HFEVDLGFEHFFTGHAGLLGIHRGPSAPAQTPEEARFLEAMAWPDNLEKYLENTRENI
                     RKLMGWLGRIEGAIPVERLQLWSEGEENFESRLEEIAAAS"
BASE COUNT         1721 a         2145 c         2324 g         1636 t
ORIGIN      
        1 ggctgcgacg ctgatttttg cttcgagtta gttgttttcc gggcgccgct tcaggacggg
       61 ttgtttgtca gccgcggagt cgccgggagc taccggagcc tgagccgcgt taccgctgcc
      121 ggttgcggta ggcgttgggg cggtgctttc gggattgtag gccattcgca tgtatcgcgc
      181 cgcttcttcc tgcgcgccgg ccgtatcgtc cttggtatgc tgtgcgagat tgtactcatt
      241 gacggcgcgt tcgcgctgcc cgagcacatc gtaaattttc ccaatataaa tgtgggccca
      301 gacttccacc catttgggat tcagatcgcc gcccaacgct gagcgaaacg cgtttgcggc
      361 cgcctggtaa tttttctgat agaacatcgc ttcgccgatg cggaagtgag cgagtgagtc
      421 cgtcggctgg atatccagcg cgtgctgata ttcctgaatc gcttcgtaat atttgccgac
      481 gagcgccagc gcttcgccgc gagcgattgt cgcgcgaatg cgcaattgcg ggctggattt
      541 gagcaggttg ttatttgggt cgatggtgat gccgttcggt ttcgggcgtc caaacgtatc
      601 aagcttaaat tcggatgtgg tccccgtgac gagaactttt ttgtattccg gatttccctc
      661 ggtatcgact ttcacttcaa ccggcatgcg aaacgtgtcc aattcctgat gaattttgcc
      721 aattacttcg aaacctttgg gcgtccgata aacaatgtat tgcaggctga attcgggaat
      781 gcccgtggaa ttcagccatt gagaaaaaaa ggagacgaga ttgaggtgcg ggtcgcccgg
      841 tttaggcgcg gggactttcg tcgcagtcag tttctcaaat tcatcgatgg tggcagtttt
      901 gcccgcatgc tgcttgtaga aatcgcgaag aagagcggtg aatgcgtcat cgcctagctg
      961 agcgcgaagc atgtgaaaaa ccatggcgcc tttatccgcg acaatcgagt gatactggtc
     1021 ggagtacgcg ccgatgcgac tggcgtttgc gattggcgtg tcctgttcaa acatcagtgc
     1081 gccgacggca aaatcctcga gcgctttgtt gagcgcggcg acgccaccgg attcttccgc
     1141 gtacatggct tcggcataac gcgaaagccc gtcagtaatc cacacatcgg aagccgtggc
     1201 gggcaggact tggtatcccc accattggcc agccgcaagc tgtgacagta agcgatcatt
     1261 taccctggga gtccattcgc gcgcgctgat cagcagcagg ccgggggcag aatatccagg
     1321 aagcgaacca tccggcattt ccgcgacagt gatactcggc tgagttgcaa atgcaccgaa
     1381 agtgtcagag aaatatccca tgatttgcgc gagcgagttt ccgtacgccg cagcagtgga
     1441 agcttgcgcc ggtggagtgt aaaacgaaaa ttggtcgccc tgcgcggata ccggcgagag
     1501 ttgcagattg ccggccacga aggagcccac ggggccagcc tggtcgcaat ggaaaacgta
     1561 agacgactga ttctgagcgc caacgccgat tccggggtgc gaaaccgggg gatccgcttt
     1621 tcctgtgccc gcaacggtga acgtgcttgg cacgatgaca ttgaaggtgc ctgtgtaacg
     1681 gttcgacggg aaatcggtaa gtggaaacca gcgagccgga agcaagagat aggccgaggt
     1741 tttgtcaacc gacgcaaagc gcacaccttt tgtggggcta tcgtccgcgc tcgaaaccgg
     1801 cccgttgtat tcaaacgtca atgtgatttg ctttcccggt gtcgcagcgc cctgcagctc
     1861 aacggttagc agtagaggcg agttgttgtc gcgcccgaaa ttgagcgcat gcccatcggc
     1921 ggacttcacg gaagtaaccc gcaaatcggg atgaagttcg acaagcagcg tttttgcaac
     1981 ctgatcggcg acgaagtcca ccttggcttg cgcttggatc gtctgatcgt tcggattaac
     2041 gatcacttcg acgtcgtaat tcgtagcccg aaaaggaaac gcgtgcgggg cttgcgctgt
     2101 cgcggcggac gccgctagaa gagacaagat cagagcagcc gtcaattgat acgctttgaa
     2161 caagcgggtt cctccggaag cgggcgatcc agcttcagct tattttaaga taccagataa
     2221 gtacttgcag ttgcccgcga gccagatgga tttacagcat tcgagcgaaa atatccgcgg
     2281 ctcgttcgat ggcgccacca gagccgagct tggcgcggac ttcggcgagt cccgttttca
     2341 tctcatcgcg ggtttccagc gattccagaa gatagcgaac ttcggcggcg acggcccgcg
     2401 gcgtgaaatc gtcctgaatc aactcgggta ccacgcggcg ccctgccacc aaattgacca
     2461 tgctgaaaaa aggcgtatgt atcattcgcc gcaggatcgc ggccgttgtt ttggcgactc
     2521 ggtagatgac caccatcggg gtgcccagca gcgctgcctc aatggtggcc gtaccgctgg
     2581 ccacaatcgc gcaatcggca gcggccaacg catcataagc cgcgcctttt attcttttga
     2641 ggtttatgcc cgggtaattg tcatatgcag agaaaaggcc ctcgtccaat ccgggtgcta
     2701 ctgcgtgaat gaactgcaag ctgccgagcg gcttcatgtc gcgtgccagc agctggcagg
     2761 cctctagaat cggcggataa ttccgcgcga tttcgttcct gcggctgccc ggaagcagag
     2821 tcacaatggg gcgctccgga tcaagctcgc agcgtgccgc gaattcctcg cggttcatgg
     2881 ttggccggac cacgtccgcg agagggtggc cgacaaaatc cacgggaact ccagcgtcgc
     2941 gatagatctt ctcctcgaag ggaaagatca cgaccatgcg atttacgagc cgtctgacaa
     3001 cgcgaacgcg tccggcgcgc cacgcccata cttgcggccc gatgaaatag ccaattggaa
     3061 tgccggcgtt tttcacgcga cgcgcaactc ccagatgagt gcccggcgag tcgacaagta
     3121 ttgcgaggga ggcgcggcgg cgagaggctt ccgccgccag cttgcgctgc acacgcacga
     3181 cggatggaat tttatgcagc acctcggaga tgccgacgac tgcaacttcg tggtagtcgg
     3241 caatcagttc gacgccggcc tcagccatgc gagggccgcc catgccgaag agttgggcgc
     3301 cggtgcgctg gcgcagcgcg gttgcgaggc gcgctgcata catatcgctc gaggcttcgc
     3361 cggcggagat gaggattcga ggcggcgtca tgctgatggg gtgcgtgcga cgcgtgccgc
     3421 gtcaatcatc ggcaggcaag ggctgcgaaa atccagatag cgcggctccg gcaagcggcg
     3481 ctgaaagttc ctgctccttg tattggagct gatagaggcg gtagtagatg ccgtgaacgg
     3541 caaggagttc ctggtgcgag ccttgctcgc gcaggcgtcc tttatggaac acaaggatgc
     3601 ggttcgcgtg ctggatggtg ctcaagcggt gcgcgatcac gatggcagtg cggccagtga
     3661 gcaaacgatc gacagcttcg cggatcagca gctccgtctt cgtatcgacg cttgacgtgg
     3721 cttcgtcgag aatcaggaat ttcggatcgt gcgcgagggc gcgcgcaaag ctgaccagct
     3781 gccgttgacc aaccgaaaga gtcccaccgc gttcggtgac cggcgtttcc actccctgtg
     3841 gcagagattc gagaagaggt ccgagcccta cttccctaag cgctcgctcc gtgacggggc
     3901 gttctattgt ttccgtgccc aggcggacat ttgattcgag cgtgccagtg aaaaggaagg
     3961 ggtcctggag aacgattccg aacatcagcc gcaactgctg cagatcaatc tcccgaatat
     4021 ccacaccatc gagaagaatc tggccgcgct ggatgtcata aaagcgcagc agcaactgaa
     4081 taatggtggt cttgcccgcg ccagtgtgcc caacgatggc caggctctgg cccgattcta
     4141 cacggaacga aacatcccgc aggacccaat cttcctcttt cggagctggt ccaccgcgat
     4201 aagcgaacca aacgttgcga aactcaatct cgccgcgcgg cgcaggcaag gagtgtgtgt
     4261 gagcggccga aacgacgcta agcggctcgt cgaggagttg aaagatgcgt tcggaagccg
     4321 ccatagcagc ctgcaggata ttgaatttct cgctcaaatc ctgaatcggg cggaaaaaac
     4381 tctgggcgta gatcataaat gatatgagaa cgccaaccgc gatggtcccg ccgattacgc
     4441 gcacaccggc gacccaaaaa aggagagcga ttccgcccat actcaaaaat tcgacggctg
     4501 gaaagaaata ggagaacgca ttgatcgcgt ctttatacgc ttccatataa gcgcggttca
     4561 ggttggcgaa ttgcttccgg gccttttgtt cgcgattgaa tagctgcacg acggccatgc
     4621 cgctgatgtg ttcgttcagg aagccattga tacgggcgat cgatgtgcgg atgccccgat
     4681 ttgcgccgcg aacatgatta cggaagacat atgtgagcag aatcatcagg ggaagcggcg
     4741 aaagggtggc aagggcaagc atgggatgcc accacaaaag aaaaatggcc atgccgaaga
     4801 gttgcacgaa atcgttgagc atcgcgacca cgcccgaggc gaagaggtcg ttgagcgcat
     4861 ccacgtccgt cgtacaacgg gtcaccagcc gccccacggg gctgcggtcg aagaagctca
     4921 tcggcaggcg ctggagatgc tggaagatct ctttgcgcaa atcgtacatg gtttcctgcc
     4981 cgacggattg catgatgcgg acctgcacgt attggaaacc aaaactcgcg agcagcgtcc
     5041 ctaaatatgc cgccaccatc cacatgacgc cgatcaacgc tgcgcttctc gcaatcgagt
     5101 gccgaaagcc agggtcgata taatggtcta ccgcaatatg aaacaggtaa ggtccgacaa
     5161 tgcccatggc ggcaacggcg aacgtcatcg acaaggcgag cgccacttgc cagcggtacg
     5221 gccacatata cttcatgagg cgccgcgtga tctgcgtgtc gtagatcttg cctagctttt
     5281 cttcttcgcg aaaatcgctc attcgcgttc cagttcttcc tcgagaagct gcttatgata
     5341 aagatcggcg taataaccgc ccagccccag aagttgctcg tgggagccgc gctcgatgat
     5401 gcggccctcg cgcaggacga tgatttgatc ggcatctttc actgtggaga ttcgatgcgc
     5461 gatcaggatc gtggttcgct ggcgcatcac ctcccgcaag cggcgaagga tgcgttcctc
     5521 ggtatcggta tcgacgctgg aaagggaatc gtccagaacg agaattcgcg gccggcgaag
     5581 tatcgcgcga gccagtgacg tgcgctgttt ttgtccgccg gaaagcgtaa tgcctcgctc
     5641 gccgaccatg gtttcgaagc gctgcgggaa gctctgaatt tcaccggaga ttcccgcgat
     5701 ttcggcagct tccagaattt cttcttcgcc agattcgtcc acgccaaacg caatatttcc
     5761 gctgagcgtc tcgctaaaca ggaaagtgtc ctgtggcaca tatccgattg cccggcgcag
     5821 tgaatcgagg ggatagtcac gaatcgatcg gccgtcgagg aaaagcgtat tggcaggagc
     5881 ctcccataaa cgcgcaacca gcgcggcaag ggtggatttt ccgctccctg tgggcccgac
     5941 aatggcgagc gtggaagccg ccggaatgcg cagattaata tcatgcagaa cggcggggtt
     6001 gggcgcggcg tctgagccgg acgggtaggc aaaatttaga tggcggaatt cgatttcgcc
     6061 gcgaatctga tgtgtcgggt cggcattaac ctggacttta acctggtctg ggcgactatc
     6121 gcgctggccg ttcccgtcgg ctgctgcaac cgcagattcg tactgcgaaa gcgcgttgtt
     6181 ccgaatgttc ggattggctc tcagaagata gttgaggcgt cccatggaag cggcgccgcg
     6241 ctgaaaaatg ttggtgacaa acccaatcgc aatcatcggg aatatcagct ggatcaagaa
     6301 cccgtaaaag gacataaacg ttccgaggga taccttgtgg tccgtaactt ccatgccacc
     6361 caagccaagc aatatgacta cggtcaggcc aatcagcgcg gaaagcgcag ggaagaaaag
     6421 gctccatgac ccgatgagct tgatattgcg ttcgacgtaa tggcgattcg ccttatcaaa
     6481 cagcgcgatt tcctgttttt cctgagcgta agctcggatc acccggatgc ccgtaagatt
     6541 ttcttgcgcc cgcgtggaaa gcacgccgag cgccgcctgg atttgttcgg acaggcgatg
     6601 gatgatctgg ccaaagtagc gaacagataa agcgacgaat gggactggga tcagcaccca
     6661 tagcgtcagc atcggcgaaa gtttcaccat gaaaaatatg gccagcacca tggtggcaat
     6721 tgtggtggcg ctgtacatga tgccgggacc caaaaccatg cgcacggcgt tgaggtcgtt
     6781 ggtggcgcgc gacatcaggt ctccggtgcg atttcgcgcg tagaactccg gatccagcgc
     6841 gagaaagcgg tccagcagat cgctgcgcag atcgtattcc atctcgcggg agataccgat
     6901 caggatccat cgagtccaga aggaaaagaa tccttttacc agcatcacca cgatcaaaat
     6961 gatgcagaaa aggccgagcg catgatggct caggggcgcg taaattgcaa agagcggatg
     7021 cagaagatgc cgcgcagttc ccgaaagagt agagagcgct cgcggagagc cctgcaggca
     7081 atccacgatc atgccgataa tcaattgcgg caacgcgcca acgattcccg tgatcgctag
     7141 cgccaataat ccgacgctca ccatcttctt gtagcggccc acgtagggaa ggagcacctt
     7201 cagatggtcc caccccgatg gtctgcgctc agggcgcgcg gcggctccgg gaatcgattg
     7261 ggtaacggcg ctcatcggta agtcttgatc ttagacgcga gatgcatggg tttggcttcg
     7321 tgttttgggc gctgaagaca atatatatcg attaggttcc gttcggaaac cgcctcgttt
     7381 gagccagttg cctggcgcta agaggccgcg gcgatttctt ccagtcgcga ttcgaagttt
     7441 tcctcacctt cggaccacaa ttgcagccgc tccaccggaa tggcgccctc gattcgcccc
     7501 agccacccca tcagcttgcg aatattttct cgtgtgtttt ccaggtactt ctctagattg
     7561 tccggccagg ccatggcttc gagaaaacga gcttcttctg gggtttgggc cggcgcgctg
     7621 ggcccgcggt gaatgcccag caggcctgcg tgacctgtga aaaaatgttc gaacccgagg
     7681 tccacctcga agtggccgct ttggcgccat agctcgtcgt cataatcctc gccccggcag
     7741 aatatttcca gcgcttgcgg ctctattttt gattttccat tcccagcgtc gaacgtcgcc
     7801 aaatcccagt agccacctat ctcgaa
//
LOCUS       DATNDT010000319         6736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2303351, whole genome shotgun sequence.
ACCESSION   DATNDT010000319 DATNDT010000000
VERSION     DATNDT010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6736
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303351"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2240
                     /locus_tag="VN788_10315"
     CDS             <1..2240
                     /locus_tag="VN788_10315"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXU20963.1"
                     /translation="SYPEFQSLRNGMDASTDVIATSSLRTIFTALTPAAGIREGIKAQ
                     FVSGNYFSVLGINCNPGIAFHDGEKAVAVVSHAFWTRRLDSDPTIPGKIVNLGGTRFT
                     IVGVAPEGFYGTDLPPRMPDLWIPLATQSRVIAGSDWIHDIATRVLHVLVRRKPGITV
                     NQISRELRQIRGLEMSRDGRKSWLTATNATAFQTNMGGFRGTGTIGLILMGAVGVILL
                     IGCVNLVNLTMSRNAARKREIAVRLAIGASRRQIVQQLCAESLVLGILGGAIGFGLSL
                     LLCHWIRVGALTALDRISNEVFGGFQLNLAPDWRVFAYTLALSILTAALVGIWPALAS
                     TRTDLNSSLKSDGLGQGQRHLLLTAQIAACFILLAGAGLLFRGAWQSRSANPGFGLNK
                     ILLMNVDLTTIDGSPDVRSVVLGRVLDRTRSLPGVTSLALVDRAPFLGTGSGQFENEE
                     HKRLRCRFNAVSGGYFDTLKIPILAGRSFSQKEAQKGDAEVVVSEAAAQFYWPNQSPL
                     GRRISVGPQGGLPHSSYAVIGVAKVVRNTFLSKVDNYYLYFPKPISDAGGWVLVRTRR
                     SPEAAIPALRNALLEINPALASHSYLITLQKGPVQIQKLMTDIPGTIAFLLGLLALLL
                     ASVGVFGVVMYLVAQRIRVIGIHMALGAQRRDVIWLVLREGLSSVAGGTLIGLIGAAC
                     LSGLLAAFAKMPDLPDLTYQAGVFDPATFLIALGALALAVAAACLLPVYRATRVDPVI
                     ALRND"
     gene            complement(2520..5162)
                     /locus_tag="VN788_10320"
     CDS             complement(2520..5162)
                     /locus_tag="VN788_10320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU20964.1"
                     /translation="MIWWSRLWHRNRMEEQLEKELRFHLDQRESDLIARGQSPAQARR
                     EARLALGGPEQVKEKCRDARGTRWAEELLQDGGYALRTFRRKPGFVIVTLLILALGIG
                     ATTVMFTIVNSVLLRPLPFPEPDRLVILHGFTEDFGEFWGFSYPYFKDLNHEIRSVRI
                     AGWTYSSGTIGAPGEPEHVEGRQISAELFPILGVVPSCGRGFRSDEDRPGASPVVIIS
                     YDLWQRRFASDRAALGKKLIFDGKTYQIVGVAPPGFQLSGEADAFTPLGQSTDPRMQN
                     PAGRFIQVLGRLAPGVTLNQTQAELTLISRHLAEEYPKSNAGLSMRIHPLLQEVVNDV
                     GGTLSLLLAAVGLVLLIACVNIASLFLTRAVSRERELAMRAALGASRSRLVRQCMTES
                     AVLGLCGGLFGIVVAALSVHPFVALWPGNLPRAEEIHIDWRVLCFGAGISLLCGLLFG
                     LTPALRVPMHRLEEALRAGGRSITGNSRRLHSPFVISEIALAFVLLVSAGMLGHTLLT
                     LSSLNPGFNAQNVLAARFALSPKVLDNPSQIRSAWRDVLDRTRHLPEVEFAALADIIP
                     MREGENTLPYRTSPNPPPPNQEPVALASSVTPDYLKVMGIPMLKGRFFNEHDREGNEL
                     VVVVDENLAWHAFGRKDVVGQHIWIPVRPWGAAPVEIVGVVAHVRHWGLAGDDQSRVR
                     DQIYYPFAQVPAGLLHFFSSVMSITIRTRSSPLNIVKTLQQELRGASGDQTLYEVRTM
                     EQLVGASLARQRFLSLLFGIFAGLALVLASIGIYGVLAYLTGQRTSEIGVRMAVGANV
                     RDIMRLVLWQGFKMTIAGVSIGMVAALGAAQALQHLVEGMQPVNGATFAIMIPLLMLA
                     ALLASFVPARRASMVDPVKALRQE"
     gene            complement(5159..5506)
                     /locus_tag="VN788_10325"
     CDS             complement(5159..5506)
                     /locus_tag="VN788_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXU20965.1"
                     /translation="MPALKSDLPQGTLDLLILKAVAVEPVHGYAIAQRLEQVSRGVVQ
                     VPEGSLYPALHRLENRGFLAADWKKTETGREAKFYRLTRKGRKQLEAEAASWQRLIEA
                     VGLILGMADGGAQ"
     gene            complement(5615..>6736)
                     /locus_tag="VN788_10330"
     CDS             complement(5615..>6736)
                     /locus_tag="VN788_10330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20966.1"
                     /translation="AMLALLDPAVLRRMVETWMTLDIHKHFGTEFLTGAGVGPWYSVN
                     DYAMCRMARDYVRWTGDQAWLDENVGGVRVIDRLVDYATHWRTLDVNHHGLADYGGID
                     NLLECVSTYVHEVAGLNAANVFCLRFVADLLDHRGDTGRASALRREAAELARRVLELY
                     VTGQGVWNCRLPDGRLQTVRHCYDFGTVLSTMPDDLSAVRKREMTAFFARELQTPAWM
                     RALSTHDPDATFSVRPDHQWTGAYAAWPAIALTALYHAGEDRRAYEWMRGLARTARQG
                     PIGQAHFAETAVDREAGGGARKAPSDLPWITDWACVSGSAFLEPILEGLFGIRAPLFG
                     KLSASPRFGGFDPAAELRGLNYQGKSYQVSMRGVESMEG"
BASE COUNT         1392 a         2081 c         1894 g         1369 t
ORIGIN      
        1 tctcataccc cgagtttcag agtttgcgca acggcatgga tgcatccacg gacgttatcg
       61 cgacatcgtc gttgcggacg attttcacgg cgctcacacc ggctgctgga attcgcgaag
      121 gaatcaaagc ccaatttgtc agcggcaact atttttccgt cttgggtatc aattgcaatc
      181 cgggaattgc ctttcacgat ggcgaaaaag cagttgccgt tgtgagccac gccttctgga
      241 cccgccgtct ggattcagat cccacaattc ccggcaagat cgtcaacttg ggcggcacgc
      301 gattcactat tgtgggcgtc gcgcccgaag gattctatgg cactgacttg ccgccacgga
      361 tgccggacct ttggatacct ctcgcaacgc aatcgcgcgt aattgcggga agcgattgga
      421 ttcatgacat cgctacccgg gttttgcacg tgctcgtacg tcgcaagccc ggcatcacgg
      481 tcaatcaaat ctccagggaa ctgcgccaga tacgcgggct cgaaatgtcc agagatggcc
      541 ggaagagttg gcttacggct acaaatgcca cggcttttca gacaaacatg ggcggcttcc
      601 gcggaaccgg cacgatcggt ctcatcctca tgggcgctgt cggagtcatt ctcctgatcg
      661 gctgcgtcaa tctagtcaac ttaacgatgt ctcgaaatgc ggcgcgtaag cgggagatcg
      721 cggtgcgctt ggcgattggc gccagccgcc gccaaatcgt ccagcaactc tgtgcggaaa
      781 gccttgtcct cggcattctt ggtggagcga tcggctttgg tctttcattg ctgctgtgcc
      841 actggattcg ggttggcgct cttactgcgc tggatcggat ctccaatgaa gtattcggag
      901 ggtttcaact gaatctcgcg ccagattggc gcgtgtttgc gtacactctg gcgctctcaa
      961 ttcttacggc cgcgcttgtc ggaatttggc ctgccctggc ctctacgcgg acggacctca
     1021 attcatccct gaaaagcgac ggtctagggc agggccaacg tcatctcctt ctgacggcgc
     1081 agatagcagc ctgtttcata cttctcgctg gtgcgggcct gctgttccgg ggcgcttggc
     1141 aatctcgatc cgcgaacccg gggtttggcc taaataagat tttgctgatg aacgtggatc
     1201 ttacaaccat cgatgggtcc ccggatgtcc gcagtgtggt tctcggacgc gttctcgatc
     1261 ggacgcggtc ccttcccgga gttacatcgc tcgcgctcgt ggatcgcgcg ccttttttgg
     1321 gtaccgggtc cggtcaattc gaaaatgaag aacacaagcg actcaggtgc cggttcaacg
     1381 cggtctctgg cggatatttt gacacactga aaattccaat cttagccgga agaagtttct
     1441 cccaaaaaga agcgcaaaaa ggagacgcgg aagtagtcgt gagcgaagcg gcggcgcaat
     1501 tttattggcc aaatcaaagt ccgctaggcc gccgtatctc cgtcggcccg caaggcggac
     1561 ttccacattc gtcctacgcg gtgatcgggg ttgcgaaggt cgttcgcaat accttcctct
     1621 ctaaggtaga caactattac ctgtattttc ccaagccaat ctcggacgcg ggcggatggg
     1681 tactggtgcg aacgagacgc tcgccagagg cggctatacc cgctctacgc aatgccctgt
     1741 tggaaattaa tccggctctc gcgtcgcact cgtacttgat cacgcttcag aaaggcccgg
     1801 ttcagattca gaagctgatg acggatattc ccggtacaat tgcgttctta ctcggcctgc
     1861 ttgctttgct tttggcatcg gtcggcgtct ttggtgtcgt aatgtacctg gtggcccaaa
     1921 gaattcgtgt catcgggatt cacatggcgc tcggcgcgca acgccgcgac gtgatctggc
     1981 tcgtcctgcg cgagggcttg agctccgtcg cagggggcac gctgatcggt ttaataggcg
     2041 cagcctgtct ttccggcctg ctggccgctt ttgccaagat gcctgactta cccgatctga
     2101 cctatcaagc cggcgtattc gaccccgcga ctttcctgat cgcgttgggg gctcttgccc
     2161 tcgcggtggc cgccgcttgc ctgttgccgg tataccgggc cacacgcgtc gacccggtaa
     2221 tcgcactgcg caacgactaa aatcacaagc ggccttgtct tccttacgtc ccctcctgca
     2281 ccccccgcgc cgacacccga tatcttttcc cctgatagtt cagccccgca actccgccgc
     2341 cggatcgaac ccgccgaagc gccgcgacgc ggtcggattc tttccggtaa cgctgtattt
     2401 caacaacaat gcgacgggcc agtcaattct tgccgcctac ctatcaatga ggtgctaagt
     2461 cggccgtcga gactgatcgt tttcgatctt gtctggcgag agcggaaagc aggtattcac
     2521 tattcttgcc gcaaagcctt tacaggatcg accatgctgg cgcggcgagc cggaacaaag
     2581 ctggccaaga gcgcagctaa catgagaagc ggaatcataa tcgcgaaggt cgctccgtta
     2641 acgggctgca ttccttcaac aaggtgctgc aacgcctgcg ctgcccccaa ggcggcgacc
     2701 ataccgatac ttacaccggc aatcgtcatt ttaaagcctt gccataacac cagtcgcatg
     2761 atgtcgcgaa cattcgctcc taccgccatg cgcacgccga tttcggatgt tcgctgccca
     2821 gtcagatatg ccagcactcc gtaaataccg atgctcgcaa gcaccaaggc caatccggcg
     2881 aatatcccaa atagaagcga gaggaagcgc tggcgggcga gagatgcgcc tacgagttgc
     2941 tccatggtac ggacttcata aagggtctga tcgccagacg caccgcgtag ttcctgttgt
     3001 aaggttttaa caatgttcag aggcgagctt cgcgttcgaa tggtgatgga catgaccgat
     3061 gaaaagaaat gcaacagccc ggcgggcact tgcgcaaacg gataatatat ttggtcgcgc
     3121 acacgcgact gatcgtcacc cgccagaccc cagtggcgca cgtgggcaac aacacctaca
     3181 atctcgacgg gagcggcgcc ccatggacgg accggaatcc agatatgttg gccaactaca
     3241 tccttacggc cgaaagcgtg ccaggcaagg ttttcgtcga cgactactac cagttcattg
     3301 ccctcccgat cgtgctcatt gaagaagcgg cctttgagca tcgggatgcc cataactttg
     3361 aggtagtcgg gcgttacgct ggaggcgagc gcgaccggct cctggttggg aggcggcgga
     3421 ttgggactgg tccggtacgg tagggtgttt tccccttccc gcatggggat gatgtctgca
     3481 agggcggcga attccacttc gggcagatgc cgcgtgcgat cgagtacgtc ccgccaggct
     3541 gaccgtatct gggagggatt atcgagcacc ttgggggaaa gcgcgaaccg ggcggccagc
     3601 acgttctgtg cattgaagcc gggattaaga gaagagagtg tcaacagagt gtggccgagc
     3661 attcctgcgg agaccagcag cacgaaggca agcgcgatct ccgatataac gaacggactg
     3721 tgcagacggc gcgaattccc ggtgatgctc cgcccgccag cgcgcagcgc ctcttcgagc
     3781 cgatgcatgg gaactcgtaa ggccggcgtt agtccgaaaa gaagaccaca taaaagcgag
     3841 atgcccgctc caaaacagag cacgcgccaa tcgatgtgaa tctcttcggc gcgtggcaag
     3901 ttacctggcc agagcgctac aaacggatgc acgctcagcg ctgccacgac gatgccaaag
     3961 aggccgccgc aaagccccag aacggcgctc tccgtcatgc attggcgcac aagccggctg
     4021 cggcttgcgc caagagccgc acgcatcgca agttcccgct cgcgagaaac cgcgcgagtc
     4081 agaaatagac tcgcgatgtt cacacacgcg atcaacagta ccagtccaac cgcggccagt
     4141 aacagcgaga gtgtgccacc cacatcgttt accacctcct gcaaaagtgg atggatccgc
     4201 atgctgaggc cggcgttcga tttggggtat tcttcggcca aatgacggct gatgagcgtg
     4261 agttcggctt gagtctggtt cagagtaacg ccgggagcca gacgccctaa gacctgaatg
     4321 aaacgccccg cgggattctg cattcgcggg tcggtgcttt ggccaagcgg cgtgaaggcg
     4381 tccgcttccc cggacagttg aaaacctggg ggcgcgacgc cgacgatctg gtacgttttg
     4441 ccgtcgaaga taagcttttt gccgagcgca gctcgatcgg aggcgaatcg gcgttgccac
     4501 aggtcgtagc taatgatgac gacggggctc gcgcccggcc ggtcctcgtc gctccgaaag
     4561 ccgcgcccgc acgagggaac gacccccaga atgggaaaca gttctgccga gatctggcgc
     4621 ccttccacat gctcaggctc acctggagcg ccgatagtac cgctactgta tgtccagccc
     4681 gcgatcctga cagatcggat ttcgtggttg aggtccttga aatagggata tgagaagccc
     4741 cagaactcgc caaaatcttc tgtaaatcca tgcaggatca ccagccggtc tggctccggg
     4801 aaagggaggg gccggagcag gacgctattg actatcgtaa acatgacggt tgttgcgccg
     4861 attccaaggg ctaagatcag cagagtgaca ataacgaaac cgggcttccg gcggaatgtg
     4921 cggagtgcat agccgccgtc ttgcagcaac tcctcggccc aacgggtgcc gcgagcatcg
     4981 cggcactttt ccttcacttg ctcgggaccg ccaagagcaa gccgggcttc gcgtcgtgct
     5041 tgcgcgggag attggccacg ggcgatcagg tcgctttcgc gttggtccag atggaagcgc
     5101 agttcttttt cgagctgctc ttccatacgg tttcgatgcc ataaacgaga ccaccagatc
     5161 attgagcgcc tccatcggcc atgccgagaa taaggccaac ggcttcgatc aatcgttgcc
     5221 aactggcggc ttccgcttca agctgttttc ggccttttcg cgtcagcctg tagaacttcg
     5281 cttcacgccc cgtctcggtc tttttccagt cggcagccag gaaaccccgg ttttcaagcc
     5341 ggtgcagggc aggatagagc gaaccttccg gaacttggac gactccacgc gatacctgtt
     5401 cgagccgttg agcgatggcg tatccgtgga caggctcaac agcaacggcc ttcagaatta
     5461 gcagatctag agtgccttga gggagatcgg atttcagcgc tggcattgat gcctcttact
     5521 tctatgggta ccgtagcaga aaatacatag aagtacaagg tgtaggtgta gttgcttgca
     5581 tatggccaac gccggaccga tggctgcatc ctcttcatcc ctccatgctc tcaacccccc
     5641 gcatcgacac ctgatagctc ttcccctgat aattcagccc ccgcaactca gccgccggat
     5701 cgaagccgcc gaagcgcgga gaagcgctca atttcccaaa caatggagcg cgaatcccga
     5761 acagcccttc cagaatcggc tccagaaacg cgctcccgga gacgcacgcc caatccgtga
     5821 tccacggcag atccgacgga gccttgcgcg cgcctccgcc cgcctcccgg tccaccgccg
     5881 tctccgcgaa atgcgcctgc ccgatcggcc cctgccgcgc ggttcgcgcc agcccccgca
     5941 tccactcgta cgctcgccgg tcctcacccg catgatagag cgcggtcagc gcgatcgcgg
     6001 gccacgccgc gtaagccccg gtccattgat gatccggccg cacgctgaac gtcgcgtccg
     6061 ggtcatgcgt cgaaagcgcg cgcatccacg cgggcgtttg cagttcccgc gcgaaaaacg
     6121 ccgtcatctc gcgcttccgc acggcgctca aatcgtccgg catcgtcgaa agcacggtcc
     6181 cgaaatcgta gcagtgccgc acggtttgca aacgtccatc cggcagccgg caattccaca
     6241 cgccctgccc cgttacatac aattccagca cccgccgcgc caactccgcc gcttcccgcc
     6301 gcagcgcact cgcccgcccc gtgtcgccgc gatgatccag cagatcggcc acaaatcgca
     6361 ggcagaacac gttcgcggca ttcagcccgg ccacttcgtg aacgtacgtg ctcacgcatt
     6421 cgagcagatt atcgatgcct ccataatccg ccagcccatg atgattcaca tccagcgtcc
     6481 gccagtgcgt cgcataatcc accagccggt cgatgacgcg cacgccgcca acgttctcat
     6541 cgagccacgc ctgatcgccc gtccagcgca cgtaatcgcg cgccatccgg cacatcgcgt
     6601 aatcgttcac cgagtaccac ggccccacgc ccgcgccggt cagaaactcc gtgccgaaat
     6661 gcttgtggat gtccagcgtc atccaggtct cgaccatgcg ccgcagcacc gccggatcga
     6721 gcagcgccag catcgc
//
LOCUS       DATNDT010000320         3777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1223737, whole genome shotgun sequence.
ACCESSION   DATNDT010000320 DATNDT010000000
VERSION     DATNDT010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1223737"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..989)
                     /locus_tag="VN788_10335"
     CDS             complement(<1..989)
                     /locus_tag="VN788_10335"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXU20967.1"
                     /translation="MDRIKHLVVLMMENRSFDHMFGFLAGPDYPIDGLSGTESNPNSQ
                     EQQVPVTDEADFSGELTPDPGHHFPDVNMQIFSNFEGTNDGGPLMQGFVRSYEFHTHN
                     TPKAAHIMNCFEPKKIPVLTTLARQYAICDRWFSSVPGPTLPNRSFMHAATSIGRVDM
                     SPIWRDESTTIYELLDKFGLSAKIFYHDSTMAMTFKMLANGQSKWFGLIDDFYRACKL
                     DTLPAYSFIEPRYFAQDQGGQVFQASDQHPDHNITEGEQLIKDVYNAIRGNQQVWEST
                     VLAVIYDEHGGLYDHVTPPATVNPDGLIAQNPGENVAQIPPFDFTRLGLRVPAV"
     gene            complement(1087..1707)
                     /gene="ribA"
                     /locus_tag="VN788_10340"
     CDS             complement(1087..1707)
                     /gene="ribA"
                     /locus_tag="VN788_10340"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HXU20968.1"
                     /translation="MIHKKENTVALGLEKVAEADFPTRFGHFRIFGFRYQNGNKTEEA
                     VVLTMGNIASGPPPLVRIHSQCLTGDVFHSLRCDCRLQLELSLKRVAEEGRGIIVYEH
                     QEGRGIGLLNKLRAYELQDGGADTVEANELLGFEADLRDYEMPAEILRYLDVIKVRLL
                     SNNPDKIAALERAGIQVSERIPCIVEPHESTEEYLRTKKQKMGHLL"
     gene            1743..3059
                     /locus_tag="VN788_10345"
     CDS             1743..3059
                     /locus_tag="VN788_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXU20969.1"
                     /translation="MLQQQHLVVLLVKLAVAASLASILTRFNAFQQMLMREERTLMQR
                     VQLALSFSILFGAGVATRVLIPAYQAVDLGLEGSLAAGLVGGYVTGLLSGVLISIPAM
                     FNGELMSMPLFAGIGVLGGLLRDVAQDKEDVWRFSPFLDLTLSRLLRGRQNFRRSAFQ
                     LLCLASIVLAELLRLFVAKLFDGKGVFLLEQNWKDPSVLTVFATYLCTVLCVTLPLKI
                     WNNTRNEEKLEAQQASLTEARLAALTRQINPHFLFNTLNTVSSLIRTDPNKARGVVYK
                     LSSILRRLLRNTENLTPLREELSFIENYMAIELVRFGDKLRFVKEIDPDTLDRLVPSM
                     LLQPLIENSIKHGLSSKVEGGVIRVRSRLADGRLHILVEDDGVGIPESKLARLFDEGI
                     GVSNVNERLKVLFGHNCRMWIDSRPDEGTRTGIEIPEIHTALAAAS"
     gene            3132..3767
                     /locus_tag="VN788_10350"
     CDS             3132..3767
                     /locus_tag="VN788_10350"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU20970.1"
                     /translation="MSLVARTRRVYDCVSGIYPLSSYLFHSKAHRCALETAGIRDGMR
                     VLEVATGSGEMFRRLVRINRHGSTVGLDLSPNMAARTQRRIRREFPEARTLCHAVDAR
                     RMPFGDETFGAIVCCYLFELLPSDAIVLAIDECRRVLEPNGTLTLTLIGQNTAGFNRA
                     YRVCNRLAPAFWGRQVEHGVPDLIEAANFQITHDRMIRQGFYPSRVLTARK"
BASE COUNT          781 a         1037 c         1051 g          908 t
ORIGIN      
        1 acggctggaa cgcgcagccc caaccttgtg aaatcgaaag ggggaatctg cgcaacgttc
       61 tcgccgggat tctgagctat taatccatcc gggttcactg tcgcgggcgg cgttacatga
      121 tcgtacaaac ccccgtgttc atcgtaaatt accgccagca cggtgctctc ccaaacttgt
      181 tggtttccac ggatcgcgtt gtaaacgtcc ttgatcagtt gctcgccctc ggtgatgttg
      241 tggtctggat gctggtcgct ggcctggaag acttggccac cttgatcctg agcgaaatat
      301 cgcggctcaa taaagctgta ggccggcagc gtatccagct tgcaggcgcg gtagaagtca
      361 tcgatcagcc cgaaccattt gctctgccca ttggccagca tcttgaaagt catggccatt
      421 gtcgaatcgt gatagaagat tttcgcgctc aagccgaact tgtcgagaag ttcgtaaatc
      481 gtcgtgctct catcacgcca gatgggcgac atgtcgacac ggccgatgga ggttgccgca
      541 tgcatgaagg agcggttcgg cagagtcggc cccggcacgg acgaaaacca gcggtcgcaa
      601 atcgcgtact ggcgggccag ggttgtcagc accgggatct ttttcggctc aaagcagttc
      661 atgatgtgcg cggcttttgg cgtgttgtga gtgtggaatt cgtagctgcg aacgaatccc
      721 tgcatcaacg ggccgccgtc gttcgttccc tcgaagttgc tgaaaatctg catattcacg
      781 tcagggaagt gatgcccggg gtctggcgtg agctcacctg agaaatcggc ttcgtcggtc
      841 acggggacct gctgttcctg ggaattcgga tttgactcgg tcccgctcag gccatcaatc
      901 ggataatctg ggccggccag aaacccgaac atatggtcga aagaccggtt ttccatcatt
      961 agaacgacaa gatgtttgat tcgatccatc actgcggcgc tccctcagct tgctctggca
     1021 attgtactgt cttctatctg tcgggagaga gcttttcgtt acgcggaaat tttgacggat
     1081 cgacgcttag agaaggtggc ccatcttctg tttcttggtg cggagatact cttcagtgga
     1141 ttcgtgcggt tcgacgatac aaggaatccg ctcggagacc tggatgccgg cgcgctcgag
     1201 cgcggcgatc ttgtcgggat tattcgacaa cagccgcacc ttgattacat ccagatatcg
     1261 gaggatttcg gcgggcattt cgtaatcgcg cagatctgct tcgaagccaa gcaactcatt
     1321 ggcttcgact gtgtccgcgc caccgtcctg taattcgtac gcacgcagtt tattcagcag
     1381 cccgatcccg cggccttcct gatgctcata gacgatgatg ccgcggcctt cttcagccac
     1441 gcgtttcaag gataattcga gctgtaaccg gcaatcgcat cgcaggctat gaaacacgtc
     1501 gccagtaaga cattgtgaat ggatacgcac caatggcgga ggtccagatg caatgttacc
     1561 catcgtgagc accaccgctt cctccgtctt atttccattc tgataacgga agccgaagat
     1621 ccgaaaatgc ccgaagcgcg tcggaaaatc cgcctcggct actttctcaa gccccaaggc
     1681 aaccgtattt tctttcttat ggatcaatac ttgcttcaat tacaatgata gtcggagccg
     1741 cgttgctaca acaacaacat ctggtggtcc tgctggtcaa gctggcggtc gccgcatcgt
     1801 tggcgagcat tctcacccgc ttcaacgcct tccaacagat gttgatgcgc gaggagcgga
     1861 ctttgatgca gcgcgtccag cttgctctga gtttctcgat cttgttcggc gccggagttg
     1921 cgacgcgcgt tctgatccct gcctatcaag cggtggatct gggcctcgaa ggaagtctcg
     1981 ccgcggggct ggttggcgga tacgtcacgg gccttctgtc gggcgttctc atctccattc
     2041 cggctatgtt caacggcgag ctgatgtcga tgccactatt tgcgggaatc ggcgtgctag
     2101 gtggtttgct tcgcgacgta gcccaggaca aggaagatgt ctggcggttc tcaccattcc
     2161 tggatctcac gctatcacgc cttttgcgcg gccgtcaaaa ttttcgccgc agcgcttttc
     2221 agcttctctg cctcgcctcc attgtccttg ccgagctgct tcgccttttc gtcgccaaac
     2281 tgttcgacgg taaaggcgtt tttcttctgg aacagaactg gaaagaccca agtgtgttga
     2341 ccgtcttcgc aacttacttg tgtacggttt tgtgcgtaac actgccactt aaaatttgga
     2401 acaacacgcg caatgaagag aaactggaag cccaacaggc gagtctcacg gaagcgcggc
     2461 tcgccgctct gacacggcag atcaatccgc attttttgtt taatacactg aataccgttt
     2521 catctctcat tcgcacagac cccaacaagg cccgtggcgt ggtttataaa ttgtcgagca
     2581 ttttgcggcg gctgctgcgg aataccgaaa acctcacgcc gctacgcgag gagctgtcgt
     2641 ttattgagaa ctatatggct attgagctgg tgcgcttcgg cgacaagctg cgctttgtaa
     2701 aggaaatcga tccggacact ctcgaccggc tggtacccag tatgctatta cagcccttga
     2761 tcgagaacag catcaagcat ggcctcagca gtaaagtgga aggcggcgtg attcgcgtcc
     2821 ggagccgctt ggccgacggc cggctgcata tactcgtgga ggacgatggc gtgggaattc
     2881 ccgaatccaa gctggcgagg ttgttcgatg aaggtatcgg tgtgagcaat gtcaatgaac
     2941 gccttaaggt cttgttcggg cacaattgcc ggatgtggat cgacagcagg cccgacgaag
     3001 ggactcgcac gggcatcgaa attccggaga ttcataccgc tctcgccgcc gcttcctaat
     3061 ttttcaatca aatcatgggt cttaattcaa cctcccttat cgccagcgtt tcagaatgcg
     3121 gcgatcttcc aatgagcctc gtcgcgcgca cgcgccgcgt atacgattgc gtatcgggca
     3181 tctatcctct ttcgagttat ttgttccact ccaaggcgca ccgttgcgcg ctggaaaccg
     3241 cggggattcg cgacggtatg agggtgcttg aagtggccac cgggtctggc gaaatgtttc
     3301 gacgcctggt gcgcattaac cggcatggtt cgaccgtggg gctggacctc tcgccgaata
     3361 tggccgcgcg aacgcaaagg cgcattcgga gagagttccc ggaagcaaga acgctatgcc
     3421 acgccgtgga tgcccgccgg atgccgttcg gcgatgagac cttcggcgca attgtgtgct
     3481 gctatctgtt tgaactgttg ccttcagacg ctatcgtgct cgcgatcgac gagtgccggc
     3541 gcgtacttga gccgaacggc acgctcacac ttactttaat tggccagaat accgctggtt
     3601 tcaaccgggc atatcgcgta tgtaaccgtc tggcgccagc attctgggga cggcaggtgg
     3661 agcacggggt tccggacttg atcgaagccg ctaattttca aattacccat gacagaatga
     3721 ttcgccaggg attctatccg tcgcgcgtgc tgaccgcacg gaaataagcc ggcgcac
//
LOCUS       DATNDT010000321         4888 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1066885, whole genome shotgun sequence.
ACCESSION   DATNDT010000321 DATNDT010000000
VERSION     DATNDT010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1066885"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..225)
                     /locus_tag="VN788_10355"
     CDS             complement(<1..225)
                     /locus_tag="VN788_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU20971.1"
                     /translation="MPKQEFYGWKLVAVLFSLDFINMGFPYYGATVINGYMVREVAMS
                     RSMIGLGFTLLNLFVGLAAVLVAISVVKFGL"
     gene            complement(333..1625)
                     /locus_tag="VN788_10360"
     CDS             complement(333..1625)
                     /locus_tag="VN788_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007958319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU20972.1"
                     /translation="MSTKRGFYGWKLVGALSAMDFLNLGFPFYIGAVINPYMLQHIQM
                     SRSTFGFGSTLLNLFVGVPSTLVAISIVKRGIRATYLIGSAFVCIGSLFLAFATTRPW
                     EFLLGFGVINGIGVCFGDIIPGSTAAARWFERYRGRAVGLVLSGSGVCGFFMAPLIDK
                     LLRASGGNWRLGWEIVAGGAVLAGIIALLFVKERPEDLGQLPDGEVKDSTGAPRQHAP
                     SALITQYEWTPGEAYRTSSFWLVVFAGLAAQFPFFIFVAHWLLHLQSVGIPSGDAAFS
                     LGLFTVACIAGRLIGGWLMDTMTARYAFMMGLCCYLIGSLAALRVGPGALWMANAAAV
                     LYGLAIGWTFTCMTTCIAHFYGPTAFPKLAGTLLLLTSGGASPAGWVGGKIFDMYGGY
                     ARAWQLNIVITVIGIVAILFATMPHHRTESRAAVARAA"
     gene            1804..3003
                     /gene="pobA"
                     /locus_tag="VN788_10365"
     CDS             1804..3003
                     /gene="pobA"
                     /locus_tag="VN788_10365"
                     /EC_number="1.14.13.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018659 - 4-hydroxybenzoate
                     3-monooxygenase activity [Evidence IEA];
                     GO_process: GO:0018874 - benzoate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate 3-monooxygenase"
                     /protein_id="HXU20973.1"
                     /translation="MKVRTQVGIVGAGPAGLLLSHLLHLQGIESVVVENRSRQYVEER
                     VRAGVLEQGTVDLLKETGVGERLKREGLTHYGVRLRFGGRTHRIDFEELANGRTVTIY
                     PQNKVVGDLFDARAAAKGQVFVEAVDVSVHDFGVSGANGSGKPKIRFRIGGESHEITC
                     EFIAGCDGFHGVCRPSIPEGALKVYDRVYPFGWLGILAEAPPSSEEIIYAYHERGFAL
                     LSMRSPELSRLYVQCAPDEDINEWPDERIWQELHARFATVENWKLTEGRIIQKGVTGM
                     RSFVVEPMQYGNLFLAGDAAHIVPPTGAKGLNLAVSDVRVLAPAFAEFYKTGKRNLLK
                     TYSETCLRRVWKVQRFSWWMTSMLHLFDTDNAFDQRRQLAELDYVTSSRAASQTLAEN
                     YVGLPME"
     gene            complement(3287..4345)
                     /locus_tag="VN788_10370"
     CDS             complement(3287..4345)
                     /locus_tag="VN788_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HXU20974.1"
                     /translation="MKNALLAVIAALCVFLYTGTGAKAQDQAAPPPPGAPTTAAAAPG
                     ATAPQGRKKRIAVFDFDYATVQTASSAAFGTNVDVGKGITDLLVKYLVQDGTYSVIER
                     QALDKIMAEQNFSNSDRANPTSAAKLGKLLGVDAIIVGAVTQFGNDTKNQNIGGGGGN
                     WGGFGVGGFSHKKTKAIVNIDARIVDIDTAEILGVAEGHGESSRSSTGLLGGGGNWHG
                     WGNGAVDFGSSDFQQTILGEAVKAAVEQMSTGLVADNTKLQTRTIVVSGLVASVDGGQ
                     IVLNVGGKAGLKVGDQLSVERVTKEIKDPATGAVIRRLASPVGVVRVTDVDDISAVAA
                     PVSGAGFKVGDAVKTVTQ"
     gene            4561..>4888
                     /locus_tag="VN788_10375"
     CDS             4561..>4888
                     /locus_tag="VN788_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20975.1"
                     /translation="MSSNIFSRIRVSVSVLALAVAMAITPAAIAQAPAGSPPQGQSAA
                     AAGAAAAAAAMKNPFPPFHMIGNIYYVGSAGLSCYLINTPRGNILLDTGYPDMAPQIE
                     GNIEKLG"
BASE COUNT         1038 a         1555 c         1341 g          954 t
ORIGIN      
        1 cagcccaaat ttcaccacgg atatagccac aagcaccgcg gccagcccca cgaacaaatt
       61 gagcagcgta aacccgaggc cgatcatgct cctgctcatc gcaacctcgc ggaccatgta
      121 gccattgatc accgtcgcgc cgtagtaagg gaatcccatg ttgatgaagt ccagcgaaaa
      181 taggacggcc accaatttcc aaccgtaaaa ttcttgcttc ggcatcagag attccctccc
      241 atcttcatgc agcttgcgca acgaccgcga gcttccagcc cataaaactt ggccttatcg
      301 ggcacccgag tctcctttgc gaatctccga cctcatgcgg cgcgagcgac cgccgccctg
      361 ctttcagtac gatgatgcgg catcgtagca aataggatgg cgacgatgcc gatcaccgtg
      421 atgacgatat tcagttgcca ggcgcgagcg tagccgccgt acatatcgaa gatcttcccg
      481 ccgacccagc ctgctggtga cgcgccgccg gaagtgagaa gcagcagcgt gccagcgagt
      541 ttcggaaacg ctgtggggcc gtagaagtgc gcgatacaag tggtcatgca agtgaaggtc
      601 cagccgatgg cgagtccata gaggaccgcg gcggcattgg ccatccatag ggcgcctggg
      661 cccacacgca gcgcggcgag cgaaccgatc agataacagc acagccccat catgaaggca
      721 taccgcgccg tcattgtgtc catcagccac ccgccgatca atcggccggc gatgcaggcg
      781 acggtaaaga ggcccagaga gaatgcggcg tcgccggatg gaatcccgac gctctgcaga
      841 tgtagcagcc aatgcgccac gaaaatgaaa aacggaaatt gtgccgccag ccccgcgaac
      901 accaccagcc agaaactgct tgtgcgatac gcctcgcccg gcgtccattc gtattgcgtg
      961 attaaagcgc tgggggcgtg ttgccggggt gcgccggtgg agtctttcac ttctccgtcg
     1021 ggcagctggc ccaaatcctc gggcctttcc ttgacgaata gcagcgcgat gattcccgcg
     1081 agcacagctc ctcccgcgac aatttcccag cccaggcgcc aattgccgcc actggcacgc
     1141 agaagcttat caatcagtgg tgccatgaag aatccgcaga cgcccgaacc gctgagcacg
     1201 aggccgacgg cgcggccgcg gtaacgttcg aaccagcgcg ccgccgcggt gctgccggga
     1261 atgatgtcgc caaaacacac accgatgccg ttgatgacgc cgaatccgag aaggaattcc
     1321 cagggtcgcg tagtagcaaa cgccagaaac agcgaaccga tacagacgaa cgccgagcca
     1381 atcagatacg ttgcgcgaat gcctcgcttt acgattgaaa tggccaccag ggtggacggg
     1441 acgccgacga acagattcag cagtgtggac ccgaatccga acgtgctgcg gctcatctga
     1501 atgtgctgca gcatatacgg attgataacg gcgccgatgt aaaacggaaa tccgagattc
     1561 agaaaatcca tggccgaaag tgctcctacg agtttccagc cgtagaatcc tcgcttggtc
     1621 gacacttgga ctccccccga acagccgctc gccgaatcgc gcactcggaa ggcggcgctc
     1681 gcatttgcct gcccgccact caaaaagcgg gttctcgcag aagacgtgcc agcattctag
     1741 attgatctac ccgctttgca agatgaatgt agaataggcc ggttttgcga gttaatctga
     1801 accttgaaag tccgcacaca agttggaatc gtcggcgccg gaccagccgg gcttctgctt
     1861 tcccatcttc ttcatctgca aggcatcgag tcggttgtag tcgaaaatcg cagccgccaa
     1921 tatgtggaag aacgcgtgcg tgccggcgtg ctcgagcagg gaactgtcga tctgctcaag
     1981 gaaaccggcg taggcgagcg cctcaaacgc gaagggctta cccactacgg cgtccgcttg
     2041 cgcttcggcg gtcgcactca tcgcattgac ttcgaggaac tcgcgaacgg gcgcaccgtc
     2101 acgatttacc cgcagaataa ggtcgtcggc gatctcttcg atgcgcgcgc ggccgcgaag
     2161 ggccaggttt tcgtggaagc ggtcgatgtg agtgtgcacg atttcggcgt cagcggcgcg
     2221 aatggcagcg gcaagccgaa gatccgcttt cgcatcggcg gcgagtcgca cgaaatcacc
     2281 tgcgagttca ttgccggatg cgatggcttc cacggtgtct gccgcccgtc gattcccgaa
     2341 ggcgcgctga aagtctacga ccgcgtttac cccttcggtt ggctgggaat tttggccgag
     2401 gcgcctccgt catccgaaga gataatttac gcctatcatg agcgcggctt cgccctactc
     2461 agcatgcgct cgccggagct cagccgactt tacgttcagt gcgcgcccga tgaggacatc
     2521 aacgagtggc ctgacgagcg aatctggcag gaactgcacg cgcgttttgc cacggtcgaa
     2581 aactggaagc tcaccgaagg ccgcataatc caaaaaggcg tcaccggcat gcgcagcttc
     2641 gtcgttgaac cgatgcaata tggcaatctt ttcctcgcag gcgatgccgc gcacattgtt
     2701 ccgcccaccg gcgcgaaagg cctgaatcta gctgtttccg atgttcgcgt gctggcgcca
     2761 gccttcgcgg aattttacaa gaccggaaag cgcaacctgc tgaaaactta ttcagagact
     2821 tgcctgaggc gcgtctggaa ggtacagcgc ttttcctggt ggatgacctc catgctccac
     2881 cttttcgaca ccgacaacgc cttcgatcag cgccgccagc tagccgaatt ggactacgtc
     2941 accagttcgc gcgccgcttc gcaaacgctc gcggaaaact acgtcggcct gccaatggaa
     3001 tgatcgggat aaatgattga aataattgtg gattgaaaac acaggacttg tcgcgggcca
     3061 tccccgaaac accacacgca ccgtggacga tttttcttta caccaacaac tacttgcgcc
     3121 cgcttatgtg cccgcggcaa aatattgctc gtatcctcgt actgcgccca ttcggattca
     3181 tgtggagtgc ggcggctcgc cgtcgctgtt tgccgccccg ccccgggctc acccgggcgt
     3241 ggtcctcgcg cgaacaaaga tcacggtttt aacgagccga gcggtcctac tgggtcacgg
     3301 ttttaaccgc atcgcccacc ttgaatcccg ctccggagac gggcgcggcc actgccgaaa
     3361 tatcgtccac gtccgtgaca cggaccacgc cgacgggcga agccagccga cgaatcaccg
     3421 ctccggtcgc cgggtctttg atctccttcg tgacccgctc gaccgatagc tgatctccca
     3481 ccttcaaccc ggccttcccg cccacattca ggacaatctg cccaccgtct acggacgcca
     3541 cgaggcccga aacaacaatc gtgcgcgttt gcagcttggt gttatcggcc accaggccgg
     3601 tgctcatctg ctcaaccgcc gccttgaccg cctcgccgag aatggtctgc tggaagtcgc
     3661 tgctgccgaa atccacggct ccattgcccc agccgtgcca gtttcctccg ccgcccaata
     3721 gccccgtgct cgagcgcgac gactcgccgt ggccctcggc aacgcccaaa atctctgctg
     3781 tgtcgatgtc cacgatgcgc gcatcgatgt tcacaatcgc cttggtcttc ttgtgcgaaa
     3841 agcctccgac gccgaagccg ccccaattgc cgcctccacc gccgatgttc tggttcttgg
     3901 tgtcgttgcc aaactgcgtc acagcgccaa caataatcgc atccacgccc agcagcttgc
     3961 cgagcttcgc tgccgacgtc ggattggcgc ggtcgctatt ggagaaattc tgctcggcca
     4021 tgatcttgtc cagcgcctgc cgttcgatta ccgagtaagt tccatcctga accagatact
     4081 tcaccagcag atccgtgatc cctttgccca cgtccacgtt ggtgccaaac gcggcggacg
     4141 acgccgtttg cactgtcgcg tagtcgaaat cgaatacggc gatccgcttc ttgcgcccct
     4201 gcggcgcagt cgctccgggc gcggctgccg ccgtggtcgg cgccccaggc ggaggcggcg
     4261 cagcctgatc ttgagctttc gctcccgttc ccgtgtaaag aaatacgcac aaagccgcaa
     4321 ttaccgctag aagggcattc ttcatggtcg tttctcccgc tacccgatga acttggttga
     4381 ccgaacgtgg ccggaatatt actggtcgca atagatgcct gtcaataaca cagcgaagcc
     4441 ctgagtcgtc acagcaacca tttgcgcctc aactccgctt ctgctagttt gctcgcagac
     4501 cggcagtgaa acttgtttca gaagcggtct ttgctgcgcg atcgaggcaa aggggtacga
     4561 atgagctcga atatattttc acgaatccgc gtgagcgttt cagtgctcgc tcttgcagtc
     4621 gcaatggcaa ttacccctgc ggctatagcc caggcgcccg cgggatcgcc cccgcaagga
     4681 cagagtgctg ctgcagccgg cgccgcggct gcggcagccg ccatgaaaaa tcccttcccg
     4741 ccctttcaca tgattggcaa catctattac gtcggctccg cgggtctctc ttgctatctg
     4801 atcaacacgc cgcgaggcaa catcctcctc gataccggtt atcccgatat ggccccgcag
     4861 atcgaaggca atatcgaaaa gctcggtt
//
LOCUS       DATNDT010000322         6453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3477178, whole genome shotgun sequence.
ACCESSION   DATNDT010000322 DATNDT010000000
VERSION     DATNDT010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6453
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3477178"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..129
                     /locus_tag="VN788_10380"
     CDS             13..129
                     /locus_tag="VN788_10380"
                     /inference="COORDINATES: protein motif:HMM:NF012599.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HXU20976.1"
                     /translation="MLTAGPGITITIQATGKDAAAAVDALCELIASRFGEED"
     gene            complement(189..1961)
                     /locus_tag="VN788_10385"
     CDS             complement(189..1961)
                     /locus_tag="VN788_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008963221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HXU20977.1"
                     /translation="MKDTIAIDVRFRLRKRAIQTVIHACALILSATVTSLASAQQAPR
                     QPALDPTQTEKNIDAQEAERKRAKTSPVQIPKIARPEISTDTKPLFKLTGVSVKGGSV
                     LPDDAIAETYRPYIGSTVSKADLATIAAKIGDQYRDAGYYLSRAIVPPQDINRGRIHI
                     RLVEGTIAEIVLKGEGVEQFGVRPLLDAVVAEHPSRLETLERQLLLINDRPGVRVIDI
                     AIEEIGNATGRFRLVVHLKTWHVGGLIGLNNWGPPSVGPWQVYSASAFNSTFTAGDSL
                     GLNLSTVPNDPRELRFGRMSYDLPIGLNGVRLGAGALYGDVWPGDSRKSLDDHTTTKT
                     YEVRASIDPVLSRKSALRLTATFGFSDVSERTSLGTVYNDHIRTVGLAADYKMHDDLG
                     GWNYLNVFYRQGLGVFDASHSGDAFLSNSNGSGIFSVLNFSFARYQNLSDVVSVKVSS
                     SGQWASAPLLTSQQFYIGGPIFGRGYYSGDLSGDNGIAGAVELRFDQKLTDGFLKGYQ
                     LYSFIDGGAAWNYGDGRDNVLSLTSAGAGVRFYLPDELLADLTITVPLQSHSLISINH
                     GPRFLLSVSKAFEVCPNRIQMRCL"
     gene            2156..3229
                     /locus_tag="VN788_10390"
     CDS             2156..3229
                     /locus_tag="VN788_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HXU20978.1"
                     /translation="MLPQARIICAQFLLMTLVFMGAGSGATAQEGAWRVSKSSGDVSV
                     TTSGAQQVSLTDETTLRPGDNIRTGRNGRVLLVRGEESILIAPNSVIGIPEEKKEDGW
                     TTVIQRAGSILLEVEKRNVKHFEVTTPYLAAVVKGTQFQVTVNNNETHVDVFRGQVEV
                     TDFKSGQLALVLPGQSAKVSAHGPGGLSLHGAGTLSPIQQGEPRAPSVSPISKEERSA
                     LRGISNGQKLHIASPLGDVKLNIHQVTKGLAHAEIGSASLRRDKETGANAGQSNNDGG
                     NAFAKETSGGSGSISSGNSGSGLGNGGAGGLGNGLASGLGNGLGNGVGGGLNGSNSAV
                     GIGSGGLLPPGLGGIPPGQAKKH"
     gene            3298..6084
                     /locus_tag="VN788_10395"
     CDS             3298..6084
                     /locus_tag="VN788_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HXU20979.1"
                     /translation="MRQYRPHLLVLCVLAVTLLTGLHNTLQNALADMRFGWFPRQASG
                     DIVLVAIDSPSIEKIGVWPWPRRLHAELIGKLESAGVNNIVFDVDLSSPSDPASDQDF
                     IEALKKAGGLVALPAFEQLVKNNGHEKSVHVNRPLPQFADHAWPAIVNVAADSDGLVR
                     RYSLGEKLEGKYLPSLGTLLAGYLDREREPGRIDFSIAADSVPVFSYVDVLRGDPMFL
                     KTFKDKKVIIGATATELGDRFNVPRGHVIPGPVLQALAAESILQNRMLRNSSDFVTFG
                     VVGFIVLLLMVLWRRSSAGSRVAALVGLAVSAELIAILVQAKLGVILDTSLWHAVIAA
                     YLAALALDEIDFRELLGRIAERRFHRIAMSLGDGLVCADGAGLITVWNPGAVAIFGYK
                     FEEMIGQPFDRIFAPSDGLGKCAPFSILELPLEDLQAAGGKVMELEGRRENGEVFPLE
                     ACFSRWQGIDGFQYGAVLRDISVRRLEAERIRRLALYDTLTGLANRNTLHEQLSARFA
                     EAKTERCEVALLLIDLDKFKQINDTLGHACGDQVLCAVAERLNVLVKGDGLVARLSGD
                     EFAIVISGPDVTEKVKILAERTSRTFSEIPISVGARKFDVGVSIGAAIYPKDCGTAEE
                     LLGNADLALYQAKATGRGRHVFFESKIRHELESRVSLESDLEQAVERDEFELYYQPQV
                     NLGNGRLIGAEALIRWRHPDRGFVRPDDFMPVVNASSISDSVTRWVMKAACLQGRLWQ
                     QRGHDIRVGVNLSPSQLQSGDLAATVLSILKDTGFSPSLLELEVTEGIMLEDDDRAYE
                     VFREIQNLGVRIAFDDFGTGYASLTYLKKFPLNKLKIDKSFVMDLRADSDDMVIVGAT
                     ISLGKLLGLSVIAEGVKDRATADLLRSMGCEEGQGYYFGRPMPVAEFEQKFLSKGTLP
                     AFGSSAEVQAATAA"
     gene            6292..>6453
                     /locus_tag="VN788_10400"
     CDS             6292..>6453
                     /locus_tag="VN788_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HXU20980.1"
                     /translation="MTASAKLKTAAGDYIVKDIALAEFGRKEISLAETEMPGLMATRE
                     EYGPKQPLKG"
BASE COUNT         1389 a         1742 c         1908 g         1414 t
ORIGIN      
        1 atgggcttga tgatgctcac cgcggggccc ggcatcacca tcaccattca agccaccggc
       61 aaagacgcgg ccgcggccgt tgatgccctg tgcgagttga tcgcttcgcg tttcggcgaa
      121 gaagactagg gcgcgaccaa aagcgaagtc taacaagcta tccgctgtgg tgacgatcaa
      181 ttcgctacct agaggcagcg catttgaatt cgattcgggc agacttcaaa ggctttggag
      241 acagaaagca agaagcgcgg tccgtgattt atgctgatta gagaatgaga ttgcagcggt
      301 acggtgatcg tgaggtccgc cagcaactca tcgggaagat aaaaacgcac gcccgctccg
      361 gccgacgtga gcgagagaac attatctcgt ccatcgccat agttccaggc cgcgccgccg
      421 tcgatgaaac tatagagctg atagcccttt aggaaaccat ccgtcagttt ctggtcgaag
      481 cgaagctcaa cggcgccggc aatgccgttg tcgccgctca gatctccgct gtagtagccg
      541 cggccgaata tcggcccgcc gatatagaac tgttgcgacg tcagcagcgg cgcagaggcc
      601 cactgtccgc tcgacgaaac tttcacggat acgacgtcgg acagattttg ataacgtgcg
      661 aatgaaaaat tcaaaaccga aaaaatgccg gacccgttgc tgttggatag aaaggcgtcg
      721 ccactatgcg acgcgtcaaa cacgccgaga ccctggcggt agaatacgtt caggtagttc
      781 cagccgccca gatcatcgtg cattttatag tcggccgcca gtcccacggt gcggatatga
      841 tcgttgtaga cggtccctag cgacgtacgc tcggagacgt cgctgaatcc gaaagttgcg
      901 gtcagccgca gcgcagattt tcggctcaac accggatcga tgctggctct gacttcatag
      961 gttttggtcg tggtgtggtc gtcgaggctc ttcctagagt cgcccggcca gacatcgccg
     1021 tacagagcac cggcaccgag ccggaccccg ttaaggccga tcggcagatc atacgacatc
     1081 cggccaaatc ggagttcgcg cggatcgttg gggaccgtcg acaggttgag tcccaggctg
     1141 tcgccggcgg tgaaagtcga attgaaggcc gatgcggaat agacttgcca gggaccgacc
     1201 gagggggggc cccagttgtt caatccgatt aatccgccga cgtgccaggt cttgagatgc
     1261 acaacgaggc gaaaccggcc ggtcgcattg ccgatctctt caatcgcaat gtcgataacg
     1321 cgcacgcccg ggcggtcgtt gatcaacagt agttgccgct cgagcgtttc caaccgagag
     1381 ggatgttcgg ccacgaccgc atccagcaac ggccgaacgc cgaattgctc cacgccttcg
     1441 ccctttaata cgatctccgc aatcgtccct tcgacaagac gaatatggat ccggccgcgg
     1501 ttaatgtcct gcggcggcac gatggcgcgg ctaaggtaat agcctgcgtc tcggtattga
     1561 tcgccgattt tggctgcgat cgttgcaagg tcggccttgg atacggtact gccgatatag
     1621 ggtcggtagg tttcggctat cgcatcgtca ggcagaacac tcccgccctt taccgagacc
     1681 ccggtgagct tgaacagcgg tttggtatcg gtcgagattt ccggtcgggc aattttcgga
     1741 atctgcacgg gcgacgtctt cgcgcgcttc cgctcggctt cctgcgcgtc aatattcttt
     1801 tcggtctgag ttggatccag cgccggttgg cgcggcgcct gctgggcact agcgaggctg
     1861 gtgacggtcg ctgacaggat cagagcgcag gcgtggatga ccgtttggat cgcgcgtttt
     1921 cgcaaccgga acctgacatc gatcgcgata gtgtccttca caatcgcccg gccacccctg
     1981 aatagaaatc agatagccgc agtaggaatt aatatggtta acgaaacatt aatgcaccga
     2041 ttggatttgc gtgcattgaa aagtaaaaac gtcagagttt tccaataaat ttggcctcaa
     2101 gtggaaatac ggttgcatct cgggtgcgat gccgccggag cccatggagc tctccatgtt
     2161 gcctcaggcc agaattatct gtgcgcaatt tctgctaatg acgctcgtct ttatgggcgc
     2221 agggtccgga gcgaccgcgc aggaaggcgc ctggcgcgtc agcaagtcgt ccggcgacgt
     2281 ctcggtgacc acttcgggag ctcaacaggt ttcgctcacc gacgaaacaa ctctgcggcc
     2341 cggcgacaac atccgcactg gccggaacgg ccgcgtcctt cttgtccgcg gcgaagaatc
     2401 aattctgatt gcgcccaatt ccgtcattgg cattcctgaa gaaaagaaag aagacggctg
     2461 gacaaccgtc attcaacgag caggttcgat tctcctcgag gtggaaaaac gtaacgtaaa
     2521 acattttgaa gtcactacgc cgtatctcgc cgcggtcgta aagggcacgc agttccaggt
     2581 gactgtgaac aataacgaaa cacatgttga tgtgttcaga ggccaggtcg aagtgactga
     2641 tttcaaatcc ggtcaactag cgctcgtttt gccggggcag tcggcgaaag tttcggcgca
     2701 cgggccgggc ggtctgtcct tgcacggcgc ggggactttg agtcccattc agcaaggcga
     2761 gccccgcgca ccatcagtgt ctccgatatc caaagaggaa cgctctgccc tgcgtggaat
     2821 atcaaacgga cagaagcttc acattgcgtc tccgctgggc gacgtaaaat tgaacatcca
     2881 tcaagtcaca aagggtctgg cacacgcgga gataggctcc gcgtcattga gacgcgacaa
     2941 ggaaacgggc gcgaacgccg gtcaaagtaa caacgatggt ggtaatgcct tcgctaagga
     3001 gacaagcggc ggcagcggct cgatcagcag cggcaactca ggcagcggtt taggcaacgg
     3061 cggtgccggc ggcttaggca atggtttggc cagtggctta ggtaatggtt tgggcaacgg
     3121 cgttggtggc ggcttgaacg ggagcaactc tgccgtgggt atcggttctg gcggtttgct
     3181 gcctcccggt ctgggcggga tacctcccgg ccaagccaaa aaacattagc tgtcatcgcc
     3241 atttgatagg gtgccgccgt cttcgaaata tggcgtcaga cggcggggga cggaagagtg
     3301 agacagtatc ggccgcatct tcttgttttg tgcgttctgg cagtaactct gctgaccgga
     3361 ctgcacaata ctttgcaaaa cgcactcgcc gatatgcggt tcggctggtt tcctcgccag
     3421 gccagcggcg acatcgtcct ggtagcgatt gattctcctt cgatcgagaa gatcggagtc
     3481 tggccttggc ctcggcgact tcatgcggag ctgatcggca agctggaaag cgctggcgtc
     3541 aacaatatcg tgttcgacgt cgatttaagt tcgccgtcgg acccggcctc tgatcaagac
     3601 ttcatcgaag ctctaaaaaa ggctggggga ttggtcgctc tgccggcatt cgagcaactg
     3661 gtgaaaaata acggacatga aaagagtgtc catgtgaatc gtcctttacc gcaatttgcc
     3721 gaccacgcgt ggccggcaat cgtgaatgtc gccgccgatt cggacgggct ggtgcgacgg
     3781 tattcactcg gagaaaaact ggaaggaaag tatctgccct cgttgggcac gctgttggca
     3841 ggttatttag accgggaacg tgagcccggt cggattgatt tcagcattgc cgcggattcc
     3901 gtgcctgtct tctcttacgt ggatgtgctg cgcggcgatc ccatgttctt gaaaaccttc
     3961 aaggacaaaa aagtcatcat cggcgcaacg gcgaccgagc ttggcgaccg gttcaatgtt
     4021 ccgcgtggtc acgtgattcc aggccctgtt ctgcaggcct tagccgccga gtcgatcctg
     4081 caaaatcgca tgctgcgcaa ctcgtccgat ttcgtaacat ttggcgtagt tggtttcata
     4141 gtccttctct tgatggtgct gtggcgccgt agttctgccg gctcgcgagt cgcggctctc
     4201 gttggcttgg cggtttcagc cgaattgatc gctattctcg tacaggccaa gctgggcgtt
     4261 atcctcgata cgtcgctctg gcatgcagtc atcgccgctt atctcgccgc cctggcgctc
     4321 gacgagatag actttcgcga attgctaggc aggatcgcgg aaaggcgttt tcaccgtatc
     4381 gccatgtcgc ttggcgatgg cttggtgtgc gcggacggag ccggcctgat cacggtatgg
     4441 aatcccgggg ccgtggcgat tttcggctac aaattcgagg aaatgatcgg ccagccgttc
     4501 gatagaatat tcgccccgag cgatggcctt ggaaaatgtg ctcccttctc aatcctcgaa
     4561 ttgcctctcg aagatcttca ggcggcgggc ggcaaggtca tggaactcga gggacgacgc
     4621 gagaatggcg aggtatttcc actcgaggcc tgtttctccc ggtggcaggg aatcgacggg
     4681 tttcagtatg gcgcggtatt gcgcgacatt tcggtccgga ggcttgaagc ggaaagaata
     4741 cggcgtcttg ctttgtacga cacgttgacg ggcctcgcca atcgtaacac tttgcacgag
     4801 caattgagcg caaggtttgc tgaagcaaaa accgagcggt gcgaggtagc attgctcctg
     4861 atagacctcg ataaattcaa gcagatcaac gatacgctgg gccatgcttg cggcgatcag
     4921 gtgctctgtg ctgtcgccga gcgattgaac gttctcgtca aaggtgacgg cctggtcgcg
     4981 cgtttgagtg gcgatgaatt cgccatcgtg atcagcggtc ccgacgtgac tgaaaaggtg
     5041 aaaatccttg ccgagcggac gtcccgcacg ttcagtgaaa tccctatttc ggttggcgcg
     5101 cgaaagttcg acgtcggcgt cagtatcggc gcagcaatat atccaaaaga ttgcggaacc
     5161 gcggaagagc ttctcggcaa cgccgatctc gccctgtatc aggcaaaggc taccgggcgc
     5221 ggcaggcatg tcttcttcga gagcaagatt aggcacgaac ttgaatcccg ggtgtcactg
     5281 gagtccgacc tggagcaggc cgtagaacgg gatgaattcg agctttacta tcagccgcag
     5341 gtcaatctgg gaaacggcag attgattggt gcagaggccc ttattcgctg gcggcatccc
     5401 gatcgcgggt tcgtcaggcc agatgatttt atgccggtcg taaacgcgtc ttcaatttca
     5461 gacagcgtga cacgctgggt tatgaaagcc gcctgcttgc aaggccgcct gtggcagcaa
     5521 cgggggcatg acattcgcgt cggcgtcaat ctttcaccgt cacagcttca atcgggcgac
     5581 ctcgcagcaa ctgtcctttc gatcttgaag gataccgggt tttcaccttc gctcctcgaa
     5641 ttggaggtga cagaaggaat tatgttggag gacgatgatc gtgcgtatga ggttttccgt
     5701 gagattcaga accttggtgt ccgcattgct ttcgacgact ttggcacagg atatgccagt
     5761 ctgacctatt tgaaaaaatt cccgctcaac aagctaaaga tcgacaagtc cttcgtcatg
     5821 gatctgcggg cggattcgga cgatatggtg attgtcggcg ccacgataag tctgggcaag
     5881 ctgctgggct tatctgtcat tgccgaaggc gttaaggacc gcgccactgc cgacctgctg
     5941 cggagcatgg ggtgcgagga aggacagggc tattatttcg gccgccccat gccggtagcg
     6001 gaatttgaac agaagtttct gtcaaaaggc actctccccg ctttcggaag ctctgcggag
     6061 gtacaggccg ctactgccgc ttaggctagg tcaaacgact tttacggtcg gacagccatt
     6121 ttgatattct cgttgtccgt tctcaattta ttatgagtat acccttggtg ggattttcgc
     6181 ccgccattac ataaagaaat ccttatatat gcgttgcggc cgcggcggcg gcctgttata
     6241 ggctcgggcc aatcacacaa gcattttcac gcgtgttgca caggaccgtt catgaccgct
     6301 tccgccaagc taaaaaccgc cgccggcgac tacatcgtca aggacatcgc gctcgccgaa
     6361 ttcggccgca aggaaatctc gctggccgag accgagatgc ccggcctgat ggcgacgcgc
     6421 gaggaatacg gccccaagca gccgctcaag ggc
//
LOCUS       DATNDT010000323         8880 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_602766, whole genome shotgun sequence.
ACCESSION   DATNDT010000323 DATNDT010000000
VERSION     DATNDT010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8880)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8880)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8880
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_602766"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VN788_10405"
     CDS             <1..1083
                     /locus_tag="VN788_10405"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXU20981.1"
                     /translation="YFSSYESTDAAQVDGHLMAVSARISGYISKVNVDDNQFVHKGDV
                     LAEIDPRDYQVAVDQARADVATAQATAHSLGIDVPVTSISTTNQVSTTEADVQNAEAG
                     IGVALQQADAAQAQLAQAQANNIKAQSDLGRYKQLVDKQEISRQLYDQAVATAAEETA
                     AVSSAKAAVAGSSQQVAQARARLAQAQAAWRSSQTGPQQVASVRARALSAEATVKAKE
                     AALEAAELNLQYAKVLAPADGVVTKNVEVGMNVQPGQQLASVIPLNDVWITANFKETQ
                     LKYMKPGQRVEIKADANGRTYKGHVDSIAGSSGARLSLLPPENATGNYVKVVQRVPVK
                     IVLDPGENKDASLRLGMSVEPKVFIK"
     gene            1127..1771
                     /locus_tag="VN788_10410"
     CDS             1127..1771
                     /locus_tag="VN788_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20982.1"
                     /translation="MSCSICGKRPPKRFCPAKGEKICAICCGREREVTIDCPSNCPHL
                     LAAHRYEAEHRQPLPAEEFPYHDIRFPADFVYERWPVVTGIANAIIAFQIQHKDVHDS
                     AVYSALGSLAEMYRTLGSGIYYERPPDAPLPRALYAQIAQSLQEFQKNAPGHSNFPRL
                     KDSDIFQLLVFLLRIAKQESNGRPQSRAFLDFLRARFPLPAETPYQDAPRIIMP"
     gene            complement(1803..2429)
                     /locus_tag="VN788_10415"
     CDS             complement(1803..2429)
                     /locus_tag="VN788_10415"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU20983.1"
                     /translation="MSRIASAKRKPRKDATSTQFKTVAPKLAIVQAAAPEKRVRGVKL
                     KTVSTLTMGFALVCLGAFMAGRPSTSAAGPMATIPELKERAMLQGLRTATYQVNDLAR
                     AKEWYSTVLGVKPYFDQPFYVGFNVGGYELGLVPAADAGEKRAPAGIAYWGVKDARDA
                     YQHLLDFGAKAQEPVQDVGEGILIGAVHDPFGNVLGVVQNPNFKAGGD"
     gene            complement(2429..3283)
                     /locus_tag="VN788_10420"
     CDS             complement(2429..3283)
                     /locus_tag="VN788_10420"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXU20984.1"
                     /translation="MPFRWTVNPYRGCEFGCHYCYARYTHEYMELDGADFESKIYVKE
                     NAGGLVERDLAHDKIWGEHIAIGTATDPYQPAEREFGATRAILEKMAEREGLSLSITT
                     KSDQVVRDIDLLRKISARSSLTIHVTVTTVRTRLARMLEPRAPRPDLRLGAVRKLREA
                     GINVGVNAMPILPGLTDSEADLDALMRAARDSGAQWIAGNVLFLMPASWKSFMRFLEA
                     KFPKLGRQYRDWFKGYGNAPESYRKQISERVERLRRKYGLGSRPNRPETQSWHSPQLE
                     LALEKGES"
     gene            3759..4397
                     /locus_tag="VN788_10425"
     CDS             3759..4397
                     /locus_tag="VN788_10425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20985.1"
                     /translation="MPDVGILILDNDVASQNALKSVLDVENWRIRVVTRPTHAMAELA
                     TGAWNLVIANAALADPNGPLFAVLRELAQSEFVAPAERSDEPPRKRLSVLFLISSPRS
                     AEVQSMLEREGLPYLQKPYHLHDFLEKVSDLLVESGAIAESIRSIGGLGKGKKRRRTA
                     QASRDARRGIMFASREDYQMTEEEMAEFERQEEDDRKKREKELKEIKERGHA"
     gene            4488..5975
                     /locus_tag="VN788_10430"
     CDS             4488..5975
                     /locus_tag="VN788_10430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20986.1"
                     /translation="MNRRASRSLYLVLIPVLLLLCAYSCDAPVSTGYSTLKESDQVRF
                     VPGDPPALQISAHYKFENSGTSDLTFIDVALPNQRSFGREDLQVTVNGSQVTPSALSQ
                     GEQDAQTNLLRILLDPPWPRRQTRDLSIEYAFHSPTNRGPRITLDKDDFHLGSRGWFP
                     QFQAPKHVMARAPSRPDRTDYTIRVPADFLVLARGTPKGQKKIGSEIEYRFELSKDDL
                     PPYIVAGRYMQWPAGRNPQSAAFWTIQPLKGDPAPAAEEITAAWRTLQTVFGPLDKNI
                     AVPHIVESAQLRRSAQSEEGPDAAAAFPGGAIVNPAALDLGINSDQFLDMVSRALAHN
                     WFGDELAPTPDAALGIGEGLPEYATIVIEEARNGAAARRNRILRYLRRYEEARKQANE
                     TPLGLTMMTDPIAQRRIALAKAPLFFIELEDICGEGPMRSGLEYIVALLRGQEVGYDD
                     LRSALEQASNRNLAMPFRVWLNEKGIPQDFLDRYPLSSAVLQTGQ"
     gene            6087..6467
                     /locus_tag="VN788_10435"
     CDS             6087..6467
                     /locus_tag="VN788_10435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011520981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXU20987.1"
                     /translation="MVKTVISTETGPKAIGPYSQAIKANGFIYTAGQIPLDPSTGQIL
                     EGDVARQTARVLDNLKVILEAAGSSLDQAVRATVYLKDMNDFAAMNEVYARYFAKNPP
                     ARSTVEAARLPRDARIEIDLIALA"
     gene            6487..7878
                     /gene="sthA"
                     /locus_tag="VN788_10440"
     CDS             6487..7878
                     /gene="sthA"
                     /locus_tag="VN788_10440"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (B-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HXU20988.1"
                     /translation="MPDRNYDLIVIGSGPAGQKGAICAAKLQKRVAIIDRIVMLGGVC
                     VHTGTIPSKSVREAIFQLTGIAVRTFYGSSYRGNNNDISLQDLAFRVGSIISRETDVI
                     HAQLRRNGIEIFQGSARFADPHSIEVNGANEKTTLRGENILIACGTRPARSPDIPFDG
                     KRILDTDEFTSISALPRELIVVGAGVVGLEYASFMAALGAEVTLIDQRPQILDFVDRE
                     IVDALAYQLRQMGATFRLGEKVRRIGIDSDRNRVFAELESGKKVQGYVLLYAVGRQAN
                     GDRLNLESAGLQTDPRGKVKVNADFQTDVPHVYAAGDVIGFPALASTSMEQGRLASCR
                     MFGVPSEHMPERFPYGIYTIPEISTVGKTEEQLTAARIPYEVGISKFNELAKSMMLGD
                     ETGMLKLLFDRNTRKLLGVHIIGQRATEIVHIGQAVLAYGGSIEYFRDTVFNYPTLAE
                     AYKVAALDGLNKL"
     gene            complement(7912..>8880)
                     /locus_tag="VN788_10445"
     CDS             complement(7912..>8880)
                     /locus_tag="VN788_10445"
                     /inference="COORDINATES: protein motif:HMM:NF013442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate kinase"
                     /protein_id="HXU20989.1"
                     /translation="ADEVSAGLNSGELPPFLGIRIKPLTEELRTRSIRTLDIFLAQLA
                     SKARGELPKHFCITLPKVALPDEVAALTDICSHLEPMLDFEPGALRIELMIETPQAIF
                     NERGEANLLALVSAARGRCVAVHFGPYDYTASLNIAATYQPIAHLASDFARAAMQVAL
                     AGTGIRLADGPTNIMPIPPHRSTKEGPKLSTVQLEENRTAVYRGWKLHHENVRRSMAN
                     GFYQGWDLHPAQLPARYAAVYSFFLENLDLSAERLRNFVEKAAQATRVGQVFDDAAMA
                     QGLLNYFVQATNCGALKPTEVETLTGLSHSELRSGSFVKILAGRSS"
BASE COUNT         1891 a         2785 c         2377 g         1827 t
ORIGIN      
        1 tacttttcaa gttacgaatc cacggatgct gcgcaggttg acggccatct gatggcggtg
       61 agcgcccgga tttccggata catctccaag gtcaacgttg acgacaacca gttcgtgcat
      121 aagggtgatg tgcttgccga aatcgatcct cgcgattatc aagttgccgt ggatcaagcg
      181 cgggcggacg tcgccactgc ccaggctacg gcgcattctt tggggatcga cgtgcccgta
      241 acttcgatta gcaccacaaa ccaggtttcc accaccgagg cagacgttca aaatgccgaa
      301 gccggaattg gagtggccct gcagcaggcg gacgccgcgc aggcgcagct cgctcaggca
      361 caggcgaaca acataaaagc acagagcgat ctaggacgtt acaaacagct cgtcgacaaa
      421 caggaaattt ctcggcaact ttacgatcaa gctgtagcga ctgccgcgga agagactgcc
      481 gcagtctctt ccgcaaaggc ggcagtggcg ggttcttcgc aacaggtcgc tcaggcccgc
      541 gcgcgcctgg cccaggctca ggcggcgtgg cggtcatcgc agactggccc tcaacaggtc
      601 gcgtccgtgc gcgctcgcgc cctttcggcc gaggcgaccg tcaaagccaa ggaagcggcc
      661 ctcgaagctg ccgaactcaa tctccaatac gctaaggtgt tggcacccgc ggatggcgtg
      721 gtcacaaaaa acgtggaagt ggggatgaac gttcaacccg ggcagcaact ggccagcgtg
      781 attcccttaa acgatgtgtg gatcaccgct aatttcaagg aaacgcaact gaaatatatg
      841 aaacccggcc agcgtgtcga gataaaagcg gatgcgaacg gccggaccta caaaggccac
      901 gtggatagca tcgccggatc gagcggtgcg cggctaagcc tgctgcctcc ggaaaatgcc
      961 acgggcaatt acgtgaaggt ggtgcagcgc gtcccggtga aaattgttct cgaccccggc
     1021 gaaaataaag acgcctccct ccgattgggg atgtcggtcg aaccaaaagt cttcatcaag
     1081 tgaacgccgc tgactgccca agcctccaca attgtgaggc tttcagatga gctgctcgat
     1141 ctgcgggaag cgtccgccca agcgtttttg tcccgccaag ggcgaaaaga tttgcgcgat
     1201 ttgctgcggg cgtgagcgcg aagtcaccat agattgccca tcgaactgcc cgcatttgct
     1261 ggcggcgcat cgctacgaag cagagcatcg ccaaccgctg cccgccgaag aattccccta
     1321 ccatgatatc cggtttcccg cggatttcgt ctacgaacgc tggcccgtcg taaccggaat
     1381 cgcgaacgcg attatcgcct ttcaaatcca gcataaagac gtgcacgata gcgccgttta
     1441 ctctgccttg gggtccctcg ccgagatgta tcgcaccctc ggcagcggca tctattacga
     1501 gcgcccgccg gatgctccgc tgcctcgcgc cctctacgcg caaattgcgc agtccctgca
     1561 ggaatttcaa aagaacgcac cgggacactc caatttcccc cgcctgaagg attccgacat
     1621 ttttcaattg ctcgtttttt tactgcgcat tgccaagcag gaatccaacg gacgccctca
     1681 atcgcgcgca tttctcgact tcctgcgcgc tcgtttccca ctgcccgcgg agacgcctta
     1741 ccaagacgct ccacgcatca tcatgcccta gtctaaagac gtccaaaaag tccaaaagcg
     1801 attcagtccc cgccagcctt gaaattcgga ttctggacga cgccgagcac attcccaaaa
     1861 ggatcgtgca ccgctccgat cagtatcccc tcacccacgt cttgcaccgg ttcctgcgcc
     1921 ttcgctccaa aatcgagcaa gtgctgatac gcatcgcggg cgtccttgac accccaatat
     1981 gcaattcccg cgggtgctcg cttctcaccc gcgtccgcag ccggcacgag ccccagttcg
     2041 tagcctccca cgttgaagcc cacatagaac ggctgatcga aatagggctt cacacccagc
     2101 acggtgctgt accactcttt cgctcgagca agatcattca cctgatacgt tgcggtcctc
     2161 agtccttgca acatcgcgcg ctccttgagt tctgggatag tggccatcgg ccccgccgct
     2221 gacgtcgagg ggcgtcccgc catgaacgcg cccaagcaca cgagcgcgaa gcccatcgtc
     2281 agcgttgaca ccgtttttag cttaacgccg cggacccgct tttcgggtgc agcggcttgc
     2341 acgatcgcaa gtttcggcgc caccgtcttg aactgcgtgg acgtcgcatc cttgcgcggc
     2401 tttctctttg cagacgctat cctcgacatc agctctctcc cttctccaag gcaagttcca
     2461 gttgcggcga gtgccacgac tgcgtctccg gccggttggg gcgcgatccc aggccgtatt
     2521 tgcgacgcaa tcgctccacg cgctcggaaa tttgcttgcg ataactttcc ggtgcgttcc
     2581 cgtagccctt gaaccaatct cgatactgcc gtccaagctt cggaaactta gcctccaaaa
     2641 atctcatgaa cgatttccac gatgccggca tcagaaaaag cacgttcccc gcaatccatt
     2701 gtgcgccgga gtctcgtgcg gcccgcatca gggcatccag atctgcctcg ctgtccgtaa
     2761 ggcccggcaa aattggcatc gcgttcaccc ccacgttgat cccagcttcg cgcagcttcc
     2821 gcactgctcc caaacgcaaa tccgggcgcg gcgcgcgcgg ttccagcatc cgcgccagcc
     2881 gagttcgcac cgtcgtcaca gtcacatgta tcgttaacga agaacgcgcg gaaattttcc
     2941 gcagcaaatc gatatcgcgc accacctgat cggatttggt tgtgatcgag aggctcaacc
     3001 cttcccgctc cgccattttt tccaggatcg cccgcgttgc gccaaattct cgctctgccg
     3061 gctggtaagg atcggttgca gtcccgatcg cgatgtgttc gccccaaatt ttgtcgtggg
     3121 cgaggtcccg ttccactagg ccgcccgcgt tctctttcac gtagattttc gattcgaagt
     3181 cggctccgtc caactccatg tattcgtgcg tgtagcgcgc gtagcagtag tggcagccaa
     3241 attcgcagcc gcggtacgga ttcaccgtcc atctaaacgg cactcgctcc gaatggcact
     3301 cattcagtat ggattttacg gggagaagga aatactccgt cccgcgtttc gcttctgcgc
     3361 ggggggagga ggcggcgagg cgcgcaatgc caaccagttc cgtcgcgggc ttcctgctcc
     3421 ggtcaggcgc cggagggggc gcggaagcat cgcttggtag ctcacaattg ccgtggcgag
     3481 cggctgtggg gaatagcgtg agactggttt gcttcggctt tcccggctgg cgtcggagcg
     3541 tcgccggggt tggtcgaagg ataggagaag tggccattgt tgacctcgcc gcacatttgc
     3601 aatatattcg ccttatcttc gctatgtcaa gctaaatatt tcgcttttta ttcgccacga
     3661 taggcggtca tctcaaacct ggaaagccct gccaccgccg acggccctgc tgccgcaaga
     3721 gccgctatac tgaacggacc cttctaggag aggaatccgt gccggacgta ggcatcctga
     3781 tccttgataa cgatgtcgcc agtcagaatg ccctcaaaag cgttctggac gtcgaaaatt
     3841 ggagaatccg tgttgttaca cggcccacgc atgcaatggc cgaactggcc acgggcgctt
     3901 ggaatctggt cattgccaat gccgctctgg cggatccgaa cggaccactg ttcgccgttc
     3961 taagagaact agcccaatcc gaatttgtcg cgcctgcaga gcgttcagac gaacctcccc
     4021 gaaagcgtct gagcgtgctt ttcttgatct cgtccccccg atctgccgaa gttcagtcaa
     4081 tgctcgagcg cgagggtctt ccctacctcc aaaagcccta tcacctgcac gactttctgg
     4141 aaaaggtcag cgacctcctg gttgaatcgg gggcgattgc agagtcaata cgctcgatcg
     4201 gcggattggg caagggcaag aagcgccggc ggactgctca agcctcgcgc gacgctcgtc
     4261 gcggcataat gttcgcttct cgtgaggact accagatgac cgaggaagaa atggccgaat
     4321 tcgagcgcca ggaggaagat gaccgcaaaa agcgcgaaaa agaactcaag gaaatcaaag
     4381 aacgcggaca cgcgtgaagc tccgcacacc ttatgttttt ctagccccgc cgcccgccga
     4441 ttttccgcct tttaaggttc atcccacccc ggtagaatct tcgcatcttg aatcgccgtg
     4501 cttcgcgctc gctctacctt gttctgatcc cggttctcct tttgttgtgc gcatattcct
     4561 gcgacgcccc ggtctcaaca gggtacagca ccctcaagga atcggaccaa gtccgcttcg
     4621 ttcccggcga tcctccggct ctgcaaatct ccgcgcacta taaatttgaa aactcaggta
     4681 cctccgattt gacgttcatt gacgtagcgc tgccgaatca gagatctttc ggccgcgagg
     4741 atctccaagt cacggtcaac ggctctcaag taacgccgtc cgccctttcg caaggggaac
     4801 aagacgcgca gacgaaccta ctccgaattc tattggatcc gccatggcca cgcaggcaaa
     4861 cccgcgatct ctcgatcgaa tacgccttcc actcgccgac gaatcggggc ccgcgcatca
     4921 cgctggacaa agatgatttt catcttggct cccgaggatg gtttccgcaa tttcaagcgc
     4981 cgaaacacgt gatggcacgc gcgcccagcc gtcccgatcg taccgattac accattcgcg
     5041 tccctgccga ttttttggtc ctggctcggg gcactccgaa aggccaaaaa aagatcggca
     5101 gcgaaattga atatcgtttt gagctctcga aggacgattt gccgccgtac atcgtcgcag
     5161 ggcgttacat gcaatggccc gctggccgca acccgcagtc cgctgctttt tggacaatcc
     5221 aaccgctaaa aggtgatcca gcgcccgcag cggaagaaat cactgctgca tggcgcacgc
     5281 tgcaaaccgt tttcgggcct ctcgacaaaa atattgccgt tccgcatatc gtcgaatccg
     5341 cccaactgcg cagatccgct cagagcgagg aaggccccga cgctgctgcg gcgtttcccg
     5401 gtggtgcaat cgtgaatccc gcggcgctcg acttgggaat caatagcgac cagtttcttg
     5461 acatggtcag ccgcgccctc gcgcacaatt ggttcggcga cgagttggcg cccacgcccg
     5521 atgcggccct cggcatcgga gagggcttgc ccgaatatgc caccatcgta atcgaggaag
     5581 cgcgcaacgg cgccgcggcg cgccgcaacc gcatccttcg ctacctgcga aggtacgagg
     5641 aagcacgcaa gcaggcgaat gaaacgcccc tcggcctcac catgatgacc gatcctattg
     5701 cccagcgccg tatcgcgctc gccaaggccc cgctcttttt tatcgaactg gaggatatat
     5761 gcggcgaggg ccccatgcga agcggtctgg agtatattgt cgcgctgctc cgcggccagg
     5821 aagttggcta cgacgatttg cgctctgctc tcgaacaagc cagcaatcga aatctagcga
     5881 tgccctttcg cgtgtggctg aacgaaaaag ggattccaca ggatttcctc gaccgctatc
     5941 ccctatcttc ggccgttcta caaaccggcc agtgagcctg gcgtacaata tctcggtgtt
     6001 ctgcgcagcc gcgatcagca tgcaagccgt gcatatcgct ctaatcgcca tttgatctgg
     6061 ctctgtaaac tttctttcga ggaggcatcg tgaaaactgt aatctctact gaaacgggcc
     6121 cgaaagccat cggcccctac tcgcaggcaa tcaaagcgaa tggttttatt tacactgcag
     6181 gccaaattcc cctcgaccct tccaccggtc aaatcctcga gggcgacgtg gcgcgccaaa
     6241 ccgcgcgcgt tctcgataat ctcaaggtta ttctcgaagc ggcgggctcc tccttggatc
     6301 aagcggtaag agcaaccgta tatttgaagg acatgaacga ttttgcggcg atgaatgaag
     6361 tttacgcgcg ctatttcgcc aagaatccgc cggcgcgctc tacggtcgaa gccgcgcgcc
     6421 ttccgcgcga cgccagaatc gagattgatc tgatcgcgct cgcctgagcg ggcaacgaaa
     6481 cctctgatgc cagaccggaa ctatgacctc atcgtaatcg gcagcggtcc cgcaggccaa
     6541 aagggagcca tctgcgcggc taagctgcaa aagcgcgtgg ccatcatcga ccgcatcgta
     6601 atgctaggcg gcgtgtgcgt acatacaggg acgattccca gcaaaagtgt ccgtgaggca
     6661 atctttcagc tcactggaat cgccgtccgc accttctacg gcagcagtta tcggggcaat
     6721 aacaacgaca tttcattgca ggacttggca ttccgtgtcg gtagtatcat ctcccgcgaa
     6781 acggacgtca tccacgcgca gttgcgccgc aacggcatcg agatctttca gggctcggcg
     6841 cgattcgccg atccgcattc catcgaagta aatggcgcca acgagaagac caccctccgc
     6901 ggtgaaaata tcctgatcgc ttgcggcact cgccccgcgc gcagccccga tattcccttc
     6961 gacggcaaac ggattctcga taccgacgag ttcacgagca tcagcgcatt gccccgcgag
     7021 ctcatagtcg tcggcgcagg ggttgtcggc ctcgaatatg cttcgttcat ggccgcgctc
     7081 ggcgccgaag tcaccttgat cgatcagcgc ccgcagattc tcgattttgt ggatcgcgaa
     7141 attgtggacg cactcgccta tcagttgcgc cagatgggcg ccaccttccg cctcggcgaa
     7201 aaagtcaggc gcatcggcat agattccgac cgcaatcgcg tttttgcgga gctggagagc
     7261 ggcaagaagg tgcaaggtta cgtgcttctt tacgccgtcg gtcgtcaagc gaacggcgat
     7321 cggctgaacc tcgaatcggc gggccttcaa accgatcctc gcggcaaggt aaaagtgaat
     7381 gcggattttc agacggatgt cccgcatgtc tacgccgcag gcgacgtcat cggcttcccc
     7441 gcgcttgcca gcacctccat ggaacagggc cgcctcgcga gttgccgcat gtttggcgtg
     7501 ccttccgagc acatgcccga acgatttccg tacggaatct acaccattcc cgaaatttca
     7561 acggttggca agacagagga gcaactgact gcggcaagaa ttccctacga agttggcatt
     7621 tcgaaattta acgaattagc gaaaagcatg atgctgggcg acgagacggg tatgctaaaa
     7681 ctgctttttg atcgcaatac ccgcaagctc ctgggcgttc acatcatcgg ccagcgcgcc
     7741 acggagatcg tccacatcgg ccaagccgtc ctcgcttacg gcggctccat cgaatacttt
     7801 cgcgataccg tcttcaacta cccgacgctc gccgaagcct ataaagtcgc ggccctcgac
     7861 ggtctcaata aactctgatc ggctcgctaa ttcccgaccg cgccgccggc cctacgaaga
     7921 tcgccccgcc agaatcttga cgaacgaccc gctacgaagt tcggagtgcg acaaaccagt
     7981 gagcgtttcc acttcggtag gcttcagcgc gccacaattc gtcgcttgca caaaataatt
     8041 cagcagcccc tgcgccatcg cggcatcgtc aaacacttgc cccacgcgcg tcgcctgcgc
     8101 cgctttttcg acgaaattgc gaagccgctc cgccgaaaga tcgagattct ccaggaaaaa
     8161 tgaatagaca gccgcgtagc gtgccggaag ctgcgccggg tgtaaatccc atccttggta
     8221 aaagccgttt gccatcgacc gccggacatt ttcgtgatgc agcttccaac cccgatacac
     8281 cgccgtgcgg ttttcttcca gctgaaccgt gctgagcttt ggcccttctt tcgtgctgcg
     8341 atgcggcggg atcggcatga tatttgtagg tccgtcggcc agccgaattc ctgtccccgc
     8401 cagcgcaacc tgcatggctg ctcgcgcgaa atccgatgca agatgcgcaa tcggctgata
     8461 cgtcgccgcg atgttcaatg aagccgtgta gtcatacggc ccgaagtgaa cggccacgca
     8521 acgcccccga gcggcgctca ccagcgctag caaattcgct tccccgcgct cattgaaaat
     8581 cgcttgcggc gtctcgatca tcagttcgat tcgcagtgcg cccggctcga aatcgagcat
     8641 cggctccaga tgcgagcaaa tatcggtaag cgccgcaacc tcatccggca gcgccacctt
     8701 aggcagtgtg atgcagaaat gtttcggtaa ttcgccgcgc gctttcgaag cgagctgcgc
     8761 cagaaaaata tccagcgtgc gaatgctgcg cgtgcgaagt tcttcggtca gcggcttgat
     8821 tcgtattccc agaaatggcg gcagctcccc cgaattcagc ccggcgctca cttcatccgc
//
LOCUS       DATNDT010000324         5581 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1227972, whole genome shotgun sequence.
ACCESSION   DATNDT010000324 DATNDT010000000
VERSION     DATNDT010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5581
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227972"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            798..1073
                     /locus_tag="VN788_10450"
     CDS             798..1073
                     /locus_tag="VN788_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXU20990.1"
                     /translation="MPKQSVPSIQDRVLKVIATSKHVPLESVHPDSTFESLTIDSLDR
                     LNILFDLESEFDIEINDEDAKEVKDIREMIEGVTKLVEAKSASAPAE"
     gene            1076..2287
                     /locus_tag="VN788_10455"
     CDS             1076..2287
                     /locus_tag="VN788_10455"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HXU20991.1"
                     /translation="MRRVAITGVGVVSPLGLDADSFWQSLLAAKSGIGEIQSIDPAQI
                     RFKYGAEIKNYIPEDHLDAKDASFMQRFAQFAVIAAEEAVAGASIEWTAALRESAAVI
                     TGSCLGGRPIEEAGYWELFHNGRSRVHPLTIPLSMNNAGASHICMRFGVQGPAYTVST
                     ACASSTHAIGQAFWMVRSGAAPMAIAGGSEAPFFLGSLKAWEAMRVISKDTCRPFSKE
                     RSGMVLGEGAAMLVLEPLDAALARGAHPLAEIVGFGMSADASHITQPVVTGPANAMRN
                     ALRDAGLAPEQIGYINAHGTATEANDRTETAAIRCVFDRHADRLAVSSTKSMHGHTLG
                     ASGALEAVASVFALKHGKLPPTANFLSPDPACDLDVIPNQAREAQVEACLSNSFAFGG
                     LNAVLAFKSLP"
     gene            complement(2302..3294)
                     /locus_tag="VN788_10460"
     CDS             complement(2302..3294)
                     /locus_tag="VN788_10460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HXU20992.1"
                     /translation="MSVLVTGASGFLGGRLAQVLVQQGEQVTVLARPTSDLRHLAALV
                     GGPIRVVTGALTDDASLREAVRDATIIFHCAAASTDWARMSVYQECNATGTRMLLDAA
                     RHASRLRRFVHVSTTDVYGYPSIPCAETGALKDAGLPYNRTKILAEKAVWQEAQQNGL
                     PVTVVRPATIYGPRGKAFVSDIAALLRKRQMIFIDGGRATAGLLYVDDAVDAMIAAAR
                     SPAAEGQAYNLSDGSGRTWKEYVSAMARELGCKLPWINLPFGTAIGVAHTMEAPFRCL
                     PMPGRPPLTRHAVFLLGRNQDFPARKARAELDFAPKVSMNEGIARSVDWLKSVS"
     gene            complement(3297..4595)
                     /locus_tag="VN788_10465"
     CDS             complement(3297..4595)
                     /locus_tag="VN788_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HXU20993.1"
                     /translation="MSDNLVVSEIGVGEKKKFPSSSGQMFDGYFSAKHAETYKKHELI
                     DARARRLFETAALACSKDAYPFQAPLNTRSGPIVEVDGHRMLLLSSYDYLGLIGDPRV
                     DEVAIDAVRKYGTGTGGVRLLTGTLDLHREVEQALADFKGTEAAITFTSGYAANLAVI
                     SALFGPADRVIADSLSHRSLMDACRLAGVQLQRFEHNNPDSLRREIEHGPQANRTLIV
                     SDGVFSMDGDIGCLPDLIKLKKEYGCFLLVDEAHATGMLGKHGRGTDEHFGVPTDEVD
                     IWTGSLAKAIPASGGFVAVSRRLSIFLQHAAAPFIFSGAMAPASAAAALETLRILKEE
                     PERVACMKTNAAFLREGLRSLGYDTGFSQTAIIPVLMYDDTKTALFARKLRDYGVLVS
                     PVLFPAVTQGAARLRLCVTAAHTREHLEFALDVFAKLRGR"
     gene            complement(4704..>5581)
                     /locus_tag="VN788_10470"
     CDS             complement(4704..>5581)
                     /locus_tag="VN788_10470"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXU20994.1"
                     /translation="LVQTQNGTCNSSARQNMTAKLAITLRVILFSIICPLILIVAGPL
                     GRLVPSSGGPLLIGIVASLFTFALTLLFVRWEKIGLGDIGAGFSRRTAPRLLFGFAIG
                     AAIVALQDIVIYSCGHVHWVIAVSPPSFRLLFLALAGYFVLALREELAFRGYSLRRME
                     TAWGMWPALVVIGIFFTLEHAAGGWNWSRVLLGPPAGALLFGMAALATRGLAVPVGIH
                     TGFNFLQWVMGQKEVSGPFRLVVESGFAGQADAIGYAAYLVGMILAASGFWFWFRRGS
                     KCDDARAAKLRSTAL"
BASE COUNT         1288 a         1668 c         1547 g         1078 t
ORIGIN      
        1 gagtgattat acaatattca aataaattga atatagaaca gactgagagg cagaaatggg
       61 tgtaattggg tgtctaatcg ggtgcaattc tctaatcaga agcgctccag aaatggcagc
      121 cctgactgaa ccggagctcg aatttatatc acttcggcgg atttcatggc cattgcccta
      181 taaacgcgac tatggacctc aatcgcacca gggattgtgc aatgcgtaac atgcaatgaa
      241 tgaaatggtt gtacatgcga acagccacta agcggccacc acgttgctgc tgttcgagtg
      301 caaacaggcc agttcttcga aagaaaatcc gccgagattc tcagtggcta ttttgatggc
      361 tgttggttgc caacttttgt gcattttgct gcgactcatt gcaagggcca tgcctgtcgc
      421 aagtcattac tgataaatag cttatgcgcc atcgtgcgac cggttgcaac tcgctgcaaa
      481 acctcaaaaa acagactcaa aatccgccga cccttgcggt catgggggtt cgaccccccc
      541 tcccggcacc aacaaaacaa agaatttcga tcgattttgg cctctggaaa tcggagaagc
      601 taaggaccgt tgggcctcta ttggctacga gtaccgtgaa tgaacaagag ctagtgtaag
      661 cgacctcact tcaggacgat tccggctggg agaggcagcc gaacttctgc tgttgcgagg
      721 aaatcatttc agggtagaat tgacgcaact caatcccaag ggctccagtt cttcccgacc
      781 gaaaggcctt tccaaccttg ccaaagcagt ccgttccatc cattcaagat cgagtgctga
      841 aagttattgc gacttccaaa cacgttccac tcgaaagcgt tcacccagac agcacttttg
      901 aatccctgac aattgattcg cttgatcgtc tcaatatcct ctttgacctt gagagcgaat
      961 tcgacatcga aatcaacgac gaggatgcga aggaagtaaa agacattcgc gagatgatcg
     1021 aaggggtcac caagctcgtg gaagcaaaga gcgcgtccgc gccggctgaa tgaacatgcg
     1081 ccgcgtcgcc attaccggag tgggcgtcgt gagcccgttg ggcctcgatg cggacagctt
     1141 ctggcaaagc ctgctggccg caaagtcagg cattggggag attcagagca tcgaccctgc
     1201 gcagatccgc ttcaaatacg gggcagagat caaaaattac atccccgaag atcatctgga
     1261 cgcgaaagat gcgtccttca tgcaacggtt cgcccaattc gccgtcatcg cagccgagga
     1321 ggcagtagct ggcgcgagca tcgaatggac ggccgccttg cgtgaatccg cggctgtaat
     1381 taccggatca tgcctgggag gcaggccaat tgaggaagcc ggctattggg agctcttcca
     1441 caacggcagg agccgggtcc atccactcac catccccctc agcatgaaca acgccggcgc
     1501 gagccacatc tgcatgcgat ttggcgttca gggtccggca tatacggtct cgactgcctg
     1561 cgcttcttcc acgcacgcaa tcgggcaggc cttctggatg gtccgctcgg gcgcggctcc
     1621 tatggccatt gccggcggca gcgaagctcc attttttctg gggagcctga aggcatggga
     1681 ggccatgcgg gtgatcagca aggacacatg ccggcccttc tcaaaggagc gcagcggtat
     1741 ggtgctgggc gaaggcgccg ccatgctggt gctcgagccg cttgacgccg cactcgcccg
     1801 cggggcgcat ccgttggctg agattgtcgg cttcggaatg tccgccgacg ccagtcacat
     1861 tacgcaaccg gttgtcacgg gacccgcgaa cgccatgcgc aatgcattac gcgacgccgg
     1921 actcgctccg gaacagatcg gctatatcaa cgcccacgga accgcaacgg aagcgaatga
     1981 tcgtacggaa accgcggcga tccgatgcgt attcgatcgc catgccgacc ggctcgcggt
     2041 cagttccacc aaatccatgc atgggcatac tctgggggcg tccggcgcgc tggaggcggt
     2101 tgccagcgtc ttcgccctga aacacggcaa gctcccgccc accgcgaatt ttctcagccc
     2161 cgatcccgcg tgcgacctgg atgtgattcc caatcaggcg agagaggcgc aggtggaggc
     2221 ctgtctctcg aactccttcg ccttcggcgg actgaacgca gtgctcgctt tcaagtctct
     2281 tccctagtat ctgcaaccct attaggacac gcttttcagc caatccacgg agcgggcgat
     2341 gccctcattc atcgatactt taggagcgaa atccaattcc gcgcgcgcct tccgcgcggg
     2401 aaagtcctga ttccggccca gaagaaagac cgcatgccgg gtcagcggcg gccggccggg
     2461 catcggcaga cagcgaaacg gcgcctccat tgtgtgtgcc actccaattg ccgtcccgaa
     2521 cggcagatta atccatggga gcttgcaccc taactcccgg gccattgcgc tgacatattc
     2581 cttccaggtc cttccgctcc cgtcagacag gttataggcc tgtccttccg cagccggact
     2641 cctggccgct gcaatcatcg catccaccgc gtcatcgaca taaagcaatc cggcagtggc
     2701 acgaccgcca tcaatgaaga tcatctgtcg cttgcgcagc aacgccgcaa tgtcagacac
     2761 aaatgccttc ccgcgaggcc cgtagattgt agcgggccgc actacagtga caggcagacc
     2821 attctgctgc gcttcctgcc acaccgcttt ttctgcgagg attttggttc ggttatacgg
     2881 cagacccgca tccttcagcg cgcctgtttc cgcgcatgga atcgatggat agccataaac
     2941 atccgtggtg ctgacgtgaa caaatcgccg cagccgggat gcgtgccgcg cagcatccag
     3001 cagcattcgg gttccagtcg cattgcattc ctggtaaaca gacatccgcg cccagtcagt
     3061 ggacgcggcc gcgcagtgaa agatgatcgt tgcgtcccgc acagcttcgc gcagcgaagc
     3121 gtcgtctgtc agagcccctg ttacaacccg gattgggccg ccgacaagcg cggctagatg
     3181 acgcagatca gaggttggcc gggcgagtac cgttacctgc tctccctgct ggacaagaac
     3241 ctgcgcgagc cgtccgccca gaaaaccgct ggcgcccgtc accagcacag acaccgctag
     3301 cggcctcgca gcttcgcgaa cacgtccagc gcaaactcca gatgctcccg ggtatgagcg
     3361 gcagtcacgc acaggcgcag gcgcgccgct ccctgggtga ctgcgggaaa cagcacgggt
     3421 gaaaccagca ctccatagtc gcgcagcttt ctggcaaaca gagcggtctt cgtatcgtca
     3481 tacatcaaca ccggaatgat tgctgtctgg gagaaaccgg tgtcatagcc gaggcttcgc
     3541 aatccttcgc gcagaaacgc cgcattcgtc ttcatgcagg cgacccgttc gggctcttct
     3601 ttcaaaatcc tcaaggtttc cagagccgct gccgccgagg ctggggccat agcgcccgaa
     3661 aaaataaacg gagccgccgc gtgctggaga aaaatggaga gccgccgcga gacggcgacg
     3721 aatccgccgc tggcgggaat cgccttggcg agcgatccgg tccagatatc gacctcatcc
     3781 gtgggcacgc cgaagtgttc gtcggtgccg cgcccatgtt tgcccagcat accggttgca
     3841 tgcgcttcat ccaccagcag aaagcagcca tactccttct tcagcttgat cagatcgggc
     3901 aaacatccga tatcgccatc catcgagaac acgccgtcgg aaacgatcag agtgcggttg
     3961 gcctgagggc cgtgctcaat ttcgcgacgg agcgagtcgg ggttattgtg ctcaaacctc
     4021 tgcaactgga ccccggccag gcgacaggca tccatcaggc tgcgatgcga aagcgaatcg
     4081 gcaatgaccc gatcggcggg cccgaaaagt gcgctgatga ccgccagatt ggccgcgtag
     4141 cccgacgtga atgtaatggc cgcttcagtt cctttaaaat cggccagagc ctgctccacc
     4201 tcccggtgca ggtccagcgt cccggtaagc aggcgaactc cgccggttcc ggtcccatat
     4261 ttgcgcactg cgtcaatggc cacctcatcc acgcgcgggt cgccgattaa gccaagatag
     4321 tcataggacg agagcagcaa catccgatgg ccatccacct caacgattgg tccagaccgg
     4381 gtgttgagcg gcgcttggaa gggataggcg tctttggagc aggccagcgc ggccgtttca
     4441 aaaagccttc gcgcgcgagc atcgatgagt tcgtgttttt tgtaggtctc cgcgtgtttc
     4501 gcggagaagt aaccgtcgaa catctgtccc gacgacgatg gaaatttctt tttctcccca
     4561 acgccgattt cgctgaccac caaattgtca ctcatgccgt acccactata tcgtgcggca
     4621 ctcaaccagc gccaaatcgt ttttttggtc gaggcccagc cattgccggc gaggagcaga
     4681 acgtgggccg agggccaact acgctaaagc gctgtcgatc tcagctttgc ggcccgtgcg
     4741 tcgtcgcatt tgctgcctcg tctaaaccag aaccaaaagc ctgaagcagc aagaatcatt
     4801 cctacgaggt aggcggcata gccaattgca tcagcctgac cggcgaatcc agactcaacg
     4861 acaagcctga atgggcctga aacttccttc tgccccataa cccattgcaa gaaattgaac
     4921 ccggtatgaa ttccaacggg aaccgcgagg cctcgggtgg caagagcggc catgccgaaa
     4981 agaagcgctc cggcgggtgg tcccagcagg acgcgtgacc agttccaacc acccgcggcg
     5041 tgctccagcg taaagaaaat tccaataaca accagcgcgg gccacattcc ccaggccgtt
     5101 tccatgcgac gaagcgaata accgcggaag gcgagctctt cgcggagtgc gagtacaaag
     5161 taaccagcga gagccaaaaa cagaagacga aaagatggcg gggaaacggc gataacccaa
     5221 tgcacgtgtc cgcatgaata aataacaata tcctggagtg cgacaatggc ggctccgatc
     5281 gcgaagccaa agagcagccg cggcgcagtt cgcctgctga atcccgcgcc gatatcgccg
     5341 agacctatct tctcccagcg cacgaacaga agcgtgagag caaaagtgaa aagactggcg
     5401 actatgccta tcagcaaagg cccgccagat gaagggacta gccggcccag cggccctgca
     5461 acgatcagga taagagggca gatgattgaa aacaggatta cgcgcagggt aatagccaat
     5521 tttgcggtca tattctgtct tgcgctcgag ttgcaggtgc cgttttgagt ttggaccaac
     5581 t
//
LOCUS       DATNDT010000325         4508 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3238938, whole genome shotgun sequence.
ACCESSION   DATNDT010000325 DATNDT010000000
VERSION     DATNDT010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4508
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3238938"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(888..1424)
                     /locus_tag="VN788_10475"
     CDS             complement(888..1424)
                     /locus_tag="VN788_10475"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU20995.1"
                     /translation="MKKLLCVVPALLLCAMIGAPNAHADSYTPTFSCVAMPSSDFGCH
                     WIADGYTSPNVSFPSASVEVTVSSFPVFDVALPAIDTPTDAYTYEFSFEPVHIGESVG
                     TLTIDDVTTGIVSSAEDFLPGGGENDLIGNGALTFSPISASTPEPAPVILMLLGAGLI
                     FFIRKRFVSRVPIHSVSF"
     gene            2138..2779
                     /locus_tag="VN788_10480"
     CDS             2138..2779
                     /locus_tag="VN788_10480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXU20996.1"
                     /translation="MDMESRRKFVNSMMAASLAAMGGGVLFAQDFTPMGGVQIGPDGA
                     NGNPPPGPIGEGTPQESGAGGGRGRGGRGRGPRVPRPQPAGIYWISGTGIERQPGPNT
                     WDIKDHTKLTMDNVKRGVEARGGTMDSFLYMQVFFCLRSDDSTPMPTGSAAAAAYQQS
                     YTDLTAIYNTYFTSRPPRSCFALSWIPGSSLIEVVGAAYIDPSAVPPPPESRG"
     gene            2870..3469
                     /locus_tag="VN788_10485"
     CDS             2870..3469
                     /locus_tag="VN788_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXU20997.1"
                     /translation="MDMKSRRRFVTSMMLAGAASPMLFAQDGAAPGNAAAPGAAPGAQ
                     RGGRAPRVPRPHPAGIVWVSGQGANDGVPNADHPAKLKLTDPYEDHCRYTMEALKRSI
                     ERLGGTMDDFLYMQVFFCLPLDDSMPMPTGSAAAAAYQKCYSALSPIYGSYWTGPHGA
                     PPRSCFALAWIPGTSLLEMVGAAYIDPNATPAAATGAGF"
     gene            3523..>4508
                     /locus_tag="VN788_10490"
     CDS             3523..>4508
                     /locus_tag="VN788_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXU20998.1"
                     /translation="MNLKLGRRSFFSSLGAFGAGLSGLFGSSKDALAATAKTTGSMSS
                     PGLPLVDGHPIAPITHSSFCSTGNPWAELGVTPMVNIQGTVTVIGGSVMRPEVMEAIR
                     IGNMHFCVLDDLLVKSGKWLANLCKAPEGYTALVTEGCAAAILCCYAGMLTEDYNERM
                     QNIPDLRGFPKTEVIIQQGHRDNFDHQVRQTGVKLVVVETRDELVAAINERTLGIHFN
                     HIQSNRGQVPAEDVIAIAKAHNIYTFCDASADVPPKERLWELPALGFDIVAFSGGKDI
                     CGPQATGFVIGKENMLHWALLNMSPQENRIGRVCKVGKESLFGLLKAVELFV"
BASE COUNT          954 a         1255 c         1366 g          933 t
ORIGIN      
        1 ggcgccccac gatacgcccg aggcttttgc ctccgcaggg tatgagctgc tctcaaactc
       61 ggagaatgag ctggcaggaa cggaacttgg cattggaagg cgacctcccc ttgtgatgag
      121 cctagcttcc aacggtcaaa ctgccatacg cggaagcacg taaggcagac cgcaggttcg
      181 tgtattccag caaggcgctg ctatgctgtt tgcacgaaga ggacgtccag gttaaggatc
      241 gatttggaaa acgagaattc ccagcgttct ggcgaactct ccgttccagt ttacttcgtt
      301 cgacgttagc tgaccgtgcc catgccgcgc gacgcgtcgc cacagcatac gtggtagtta
      361 aagacgcccc cgtcctccga taacgaagcc caaaacggct ccacatattg tttgctgctg
      421 cctcgttgtc cccaaataat cagcccatcc attgagaact cgtgacctga cagcgtcctt
      481 tgcgaacgct ggatatttca gcgaagtcgg aagcacatcg tcaatccacc aatagcggtt
      541 ccatctttca tccgactcca acaatcgccc caagaaccta gccatcgtgg agcaaatatc
      601 gcggaccgct tccacttgct cttcatcgca gcgcaattcc catgcgcgcg catacgagcg
      661 caccgaatca aagagcattc tctctaggca agtcggttcc tggctttcca tccggcgtct
      721 ccgccgagag tctacctcaa ggcgagtcga aaggaacgga ggcttggcta gcgcgatttt
      781 tgccggtact agatcggtat atggacagga agctgacttc gcaggacttt gccgctgttt
      841 gacaaacttt gctgatacac cgcgagggct gagttcgccg cgcaggttca gaagcttacg
      901 ctgtgtatgg gcacgcgcga cacaaagcgc ttgcgtatga aaaagatcag tccagcgccg
      961 agtagcatta ggatgactgg ggctggctcg ggagtcgagg cgctaattgg cgaaaacgtc
     1021 agagcgccat tgccaattaa atcgttttct cctcctcccg gcagaaaatc ctcagcgcta
     1081 cttacaattc cggtggtgac atcgtcgatg gtgagcgtac caaccgactc gccgatgtgc
     1141 acgggttcga acgaaaattc ataggtatag gcatccgttg gggtgtcgat ggccggaagg
     1201 gccacgtcga aaaccgggaa gctggacacc gtgacctcta cgcttgccga tgggaaggac
     1261 acattcgggg acgtatagcc atcggctatc caatgacatc cgaaatcaga actgggcatg
     1321 gcgacacacg aaaaagtggg tgtataggaa tcggcgtgag cattgggagc gccgatcatg
     1381 gcgcacagga gtagtgcagg aactacgcac agcagttttt tcatggatgt cctttcggca
     1441 acaacctcaa gcttccgcga gcctacgaga agaatccaga agccgtaaat tgggaagtct
     1501 tggtaattac tgatagtaga agaccctcag tggtggttct gcgccaagtc ccgagaaacg
     1561 gcgttatcgg aaaaataccg ggctagtacg cgaagagatc gcattctccc gcgcgtgatg
     1621 cgagtcgaga cttgcgcgcc cgccggtgtc aaaggatcgt ttttatgcca gaggtggtcc
     1681 tttttccgct cccttccgct aacccttcat tgcgttgaca ggtcatttta gtgctcgtag
     1741 gagtcgcgca gggacgtacc acttttcccc tctctgtaac ctagcccacg ctcgggtatc
     1801 tgcactgcgg catccgctat gctgccggga ctcagcgacg ccctccggag aacgactcgc
     1861 tcatcgacac ggcgatcggc gatcgtttgc agcgctgcga aagttcgttg tggagggggg
     1921 cggaacagga gtaggatgcg cgcgaggcgg cggtcgaaat cggaaattcg ggatgtggta
     1981 ggatacgcgg cgttctgtgg cgtcatgtgg cctcatgggg tgtcatgtgg ctactgagga
     2041 tttgcgtttc gtgttcggcg ttgaacggca gggaattcgg ttgtgtagca atgagagcag
     2101 ggaatagagt cggcggcgaa gattcaggga gtcatttatg gacatggagt cgcggcgaaa
     2161 atttgttaac agcatgatgg cggcatcgct tgcggcgatg ggtggcggag tgctcttcgc
     2221 tcaggatttt acgccgatgg gcggagtgca gatcggccct gatggcgcca acggtaatcc
     2281 tccgcccggg ccgattggcg aaggtactcc ccaggagagc ggcgccggtg gtggccgtgg
     2341 tcgcggcggt cgtggccgtg ggccgcgtgt tccgcgccca cagcctgcgg ggatctattg
     2401 gatctccgga acggggatcg aacggcagcc cggaccgaat acctgggaca ttaaggacca
     2461 caccaaactc acaatggata acgtaaagcg cggtgtggaa gccaggggcg gcacgatgga
     2521 cagctttctc tacatgcagg tgttcttctg cctgcggtcg gatgacagca caccgatgcc
     2581 cacgggcagc gcggccgctg cagcgtatca gcagagctat accgatctaa ccgccatcta
     2641 taacacttat ttcaccagcc gtccgccgcg gtcatgcttc gcgctgtcgt ggattccggg
     2701 cagttcgctg attgaagtag tcggcgcggc gtatatcgat ccgagcgccg tacctccacc
     2761 gcccgagtcc cgcggctgag cacgtagcgg tgcggccacc ggcgtaattt ttgcggagcg
     2821 aacgagcgcg taggttcatt tcaaaagctt tcagcattgg aggtccgtca tggacatgaa
     2881 atcgagacgt agattcgtaa ccagcatgat gctcgccggg gccgcgagcc caatgctctt
     2941 cgcgcaagac ggtgctgctc ccggcaatgc tgctgcacct ggcgcagctc ccggcgcaca
     3001 acgcggtggc cgtgcgcctc gtgtgccgcg cccgcatccg gctgggattg tgtgggtctc
     3061 cggacaggga gccaatgatg gcgttccgaa tgccgaccat ccggcgaagc tgaagctgac
     3121 cgatccgtac gaagatcact gccgatacac gatggaggcg cttaagcgca gcattgagcg
     3181 gctcggtggc acgatggacg actttctgta catgcaggtg ttcttctgcc tgccgctgga
     3241 cgatagtatg ccaatgccga caggcagcgc agcggcggcc gcgtaccaga agtgctatag
     3301 cgccctcagc ccgatctacg gcagctactg gaccgggccg cacggagcgc cgccacggtc
     3361 ctgcttcgcg ctggcatgga ttcccggcac gtcgctgctg gagatggtcg gggcagctta
     3421 catcgatccg aatgcaacgc ccgcggcggc aactggtgcg gggttctaac gcttcggcag
     3481 cgacattcac gaaactcaga atgagctaca aaggatcaaa ctatgaatct gaaacttggc
     3541 cgtcgtagtt ttttctcctc gctcggcgcg tttggcgctg ggttatctgg gttattcggt
     3601 tcaagcaagg acgcgctggc ggcgactgca aagaccacgg gctcgatgag ttcgccgggc
     3661 ctgcctcttg tggatggaca cccaatcgcg cctatcaccc acagcagctt ctgctcgact
     3721 ggtaaccctt gggctgagct gggtgtgacg cctatggtca atatccaggg cacggtgacg
     3781 gtcatcggcg gatcggtgat gaggccggag gtcatggaag caatacgcat aggcaacatg
     3841 cacttctgcg tgttagatga cctgctggtt aagtccggta aatggcttgc caacctctgt
     3901 aaagcgcctg aaggctatac cgcactggtg acggagggct gcgcagcggc tatcctctgc
     3961 tgctatgccg gcatgttgac cgaggactac aacgagcgga tgcagaatat ccccgatctg
     4021 cggggctttc ccaagaccga ggtcatcatc cagcagggac atcgcgacaa cttcgaccat
     4081 caggttcgcc agacgggagt aaagctggtc gttgttgaga cgcgcgacga gttggtcgcc
     4141 gccatcaacg agcgcacgct cggcattcac ttcaatcaca tccagtccaa ccgcggacaa
     4201 gtgccggcag aggacgtcat agccatcgcg aaggcacaca atatctatac gttctgcgat
     4261 gcttcggctg acgttccacc gaaggaacgg ctgtgggagc ttccggcgtt gggtttcgac
     4321 attgtcgcct tctcgggcgg caaagacatc tgcggaccgc aggcgaccgg cttcgtgatc
     4381 ggcaaggaaa acatgctcca ttgggcgctg ctgaacatga gcccgcagga gaaccgcatt
     4441 ggccgcgttt gcaaggttgg caaggaatcg ctctttggct tgctgaaggc ggtggagctc
     4501 ttcgtcaa
//
LOCUS       DATNDT010000326         4750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1216866, whole genome shotgun sequence.
ACCESSION   DATNDT010000326 DATNDT010000000
VERSION     DATNDT010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4750
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1216866"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="VN788_10495"
     CDS             <1..473
                     /locus_tag="VN788_10495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU20999.1"
                     /translation="AIDPNNEDALLIAINSSSKRKANAKTIEYADKLVEVMNSKPKPA
                     GVSDADWDKKKNSMLGLGYWFAGVTYGTEGKYADADKALRSALPHIEGNNQLLAIGLF
                     HLGLADYKMGARSHNKKLIQDALRYSEQSEAIRSPYQAQAQANVRAIKAQLGRQ"
     gene            542..1306
                     /locus_tag="VN788_10500"
     CDS             542..1306
                     /locus_tag="VN788_10500"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU21000.1"
                     /translation="MWKGDYTFTTRCLVEKDFKIRYRNMSLGMFWSLLNPLIMMGVLW
                     FIFVKVFPGNTMPYYPLAVLCGIVPFNFFSIAWANGTSSLLDSVGLVKRVPVPRQIIP
                     IASVFSNCMHLLIQVGLLLVLVLAFGPRINIEWLWLPVLWALEIVFVCGLALIFSAVN
                     VYVRDARYFVESANNILFWLVPVFYSFSIIPARFREIYMYNPIAALVMALRNILLEGR
                     APASSLLSKLTLVSFGTLVVGWFVFKKLQERFYEYL"
     gene            1321..2073
                     /locus_tag="VN788_10505"
     CDS             1321..2073
                     /locus_tag="VN788_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU21001.1"
                     /translation="MSLIEFDHVTKTFPHAAGRVLLRTHLLRWFSSEKHTLFYALKNI
                     SFTMERGECLGIVGRNGAGKSTLLGMVAGLAKPNGGRVRVNGRVGALLELGSGFHPDL
                     SGAENLVLNASLLGIGKKRIAELFDQIVEFSGVADFINEPLRTFSSGMSLRLAFSVAI
                     HTEPDLLLIDEILGVGDSAFQAKCADALESYRRRGGSLLFVSHSGATVRKMCDRAIWL
                     DHGELMMEGNPDAVLKEYEGTSEAVAAAAQPT"
     gene            complement(2061..3608)
                     /locus_tag="VN788_10510"
     CDS             complement(2061..3608)
                     /locus_tag="VN788_10510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HXU21002.1"
                     /translation="MAGLLARKPLEMIHAEAADTGERTLKRTLGPVNLISLGIGAIVG
                     TGIFVLTGSAAALYAGPALILSFIFAGIGCVFAGLCYAEFASLIPIAGSAYTYGYATL
                     GEIFAWIIGWDLILEYAFGAATVASGWSANVVQLLERLGIYIPPQLTATPGTPLVLYH
                     KLWLPASTLPMGVSAAGLPHATGIFNIVAFLVILAVTALLIIGIEESADVNTVIVFIK
                     VGTVLVFIGVAAVFLFHHPSIAAKNWHPFIPPNTGTFGQYGWSGVARGAAVIFFAYIG
                     FDAVSTAAQEAKNPQKDMPLGILGSLVICTILYIVTSGLLTGVENYSHLNVAAPVALG
                     MSATGIAWGEYLVLIGTILGLSTVMLVMLLGQSRVFFSMSRDGLLPEWAGAIHPRFRT
                     PWISSMIVGICVAIFASLIPIGTLGELVNIGTLLAFVIVCAGVWVLRVRRPELPRPFK
                     TPFVPLVPILGIIISVLLMLALPLETWIRLIVWLVIGMVIYFAYGRRHSRVRRYIEAG
                     KDPRQVG"
     gene            complement(4096..>4750)
                     /locus_tag="VN788_10515"
     CDS             complement(4096..>4750)
                     /locus_tag="VN788_10515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21003.1"
                     /translation="HYQEDWPKLKEFLCSGANPLLCQAYRSGNIEQIFTVLDLAESLF
                     SDSQISMLKASAAQEEAEAAVAKHRSFGSEVKEYRDVRMKLLWAMEGYFLHRNHLDKQ
                     SYACPKWEVLRRFGKLLESGDIVITFNYDSTIERVLLDQEKWAPGDGYGTDLVFQQNQ
                     YDKTRVDFPPSMVKVLHLHGAIGWYSKPTFSPAFQPSAEGGGAIPREALSPARWKPK"
BASE COUNT         1064 a         1300 c         1359 g         1027 t
ORIGIN      
        1 tggccatcga tccgaataat gaagacgcgc tgctgatcgc gatcaattcg agttccaaga
       61 gaaaggcaaa cgccaagaca atcgaatatg ccgacaaact ggtggaggtg atgaactcga
      121 aaccaaagcc cgcgggcgtg agcgatgcgg attgggacaa gaagaagaat tcgatgctcg
      181 ggctgggcta ctggtttgcc ggcgttacct acggcacgga aggcaagtac gccgatgccg
      241 acaaagcgtt gcgatccgcg cttccgcaca tcgagggcaa caaccagttg ctcgcgatcg
      301 gcttatttca tttggggctc gcggattaca agatgggcgc gcggtcgcac aacaagaagc
      361 tgatccagga cgcattgcgc tactcggagc aatcggaagc gattcgcagt ccatatcagg
      421 cgcaagcgca ggcgaatgtc agggcgatta aagcgcaatt gggccggcag tgaaagcttt
      481 cacccactag aatcccgcgc cgattacaat agaaagaacg gcttcggccc ggtcctcccg
      541 catgtggaaa ggcgattaca cattcacaac gcgctgtctg gttgagaagg atttcaaaat
      601 ccgctaccgg aatatgtcgc tcggcatgtt ctggtctctt ttgaatccac tcatcatgat
      661 gggcgtgctt tggttcattt tcgtcaaggt ttttccgggg aatacaatgc cgtattatcc
      721 gctggcagtc ctctgtggca ttgttccatt caattttttt tccattgcat gggccaacgg
      781 cacttcctcg ctgctcgaca gcgttgggct ggtgaaacgg gttccggtgc cgcgccagat
      841 catcccaatc gcgagcgtgt tttccaactg catgcatctg ctgatccagg ttggtcttct
      901 gctggttttg gtgcttgcgt tcgggccgcg catcaatatc gaatggcttt ggcttccggt
      961 gctctgggcg ctcgaaatcg tattcgtctg cgggctggct ctgatcttct cggcagtcaa
     1021 cgtctatgtg cgcgacgcgc gttattttgt ggaatcggcc aataatattc tcttctggct
     1081 agttccggtc ttctattcgt tttccatcat ccccgcgcgt ttccgggaaa tctatatgta
     1141 taaccccatc gcagctctag tgatggcgct tcggaatatt cttctcgaag gccgcgcgcc
     1201 ggcgagttcg ctactatcga agctcacctt ggtttcgttt gggactctgg ttgttggatg
     1261 gttcgttttc aaaaagcttc aggagagatt ttacgaatac ctgtagcctc agaacccacg
     1321 atgtctctta ttgaattcga tcacgtcacg aagacatttc cacacgccgc gggccgggtg
     1381 ctgctgcgca cgcatctgct gcggtggttt tcatcggaaa agcatacgct tttttacgcg
     1441 ctcaagaaca tatctttcac gatggaacgg ggcgagtgcc tggggattgt gggccggaac
     1501 ggcgctggca agagcacgct cctcggcatg gtggcgggtc tggcgaagcc gaatggcggc
     1561 cgcgtcagag taaacggccg cgtaggcgct cttctggaac tgggatcggg ttttcatccg
     1621 gatctgagcg gcgcggaaaa tctggtgctg aatgcatccc tgctcgggat cggcaagaaa
     1681 cggatcgccg agctgttcga tcaaatcgtc gagttttccg gcgtcgcgga ctttatcaat
     1741 gagccgctac gcactttctc gtccgggatg agcctgcgcc tggcgttctc ggttgccatc
     1801 cacactgaac ccgacctcct gttgatcgat gaaatattag gcgtgggtga ttcggcgttc
     1861 caagccaagt gcgcggacgc gcttgaaagc taccggcgtc gcggcggatc tctgctcttt
     1921 gtgtcccact cgggcgcgac agtgcgcaag atgtgcgacc gcgcgatttg gctagaccac
     1981 ggcgagttga tgatggaagg caacccggac gcggtgttga aagaatacga agggacgagc
     2041 gaggcggtgg cggcggccgc tcagcctacc tgacggggat ccttcccggc ctcgatatac
     2101 cgccgtacgc ggctgtgtct ccggccatag gcgaaataga ttaccattcc gatcaccagc
     2161 cacacaatca gacgaatcca ggtctcgagc ggaagcgcga gcatcagcaa aacggaaatg
     2221 ataattccga gaatcggtac aagaggcacg aacggagttt tgaaaggacg cggcagctcc
     2281 ggccgccgga cgcgtagcac ccagacgccc gcgcagacaa tcacgaacgc cagcagcgtg
     2341 ccaatgttca ccaactcgcc gagcgtgccg atggggatga gcgatgcgaa aatcgcgacg
     2401 caaatgccaa caatcatcga cgagatccag ggcgtccgga aacgtggatg aatggctccg
     2461 gcccactcgg gcagcaatcc atcgcgcgac atggaaaaga acacgcgcga ctggccgagc
     2521 agcatcacca gcatcactgt acttaatccg agaatggtgc caatcaacac cagatactcg
     2581 ccccaagcga tgccagtcgc gctcatgccc agcgctaccg gagcggcaac attcaagtgt
     2641 gagtagttct caacaccggt cagtaagccc gaggtcacga tgtagaggat tgtgcaaatg
     2701 accagcgatc ccaaaatccc cagcggcatg tccttctgtg gatttttcgc ttcctgcgcg
     2761 gccgtggaga ccgcatcgaa accgatgtac gcgaaaaaga tgacagccgc tccccgcgcg
     2821 acgccagacc agccatactg cccgaacgtt cccgtattcg gcggaatgaa cggatgccaa
     2881 ttcttggcgg cgatcgaagg atggtggaaa agaaaaaccg ccgcgacgcc gataaagacc
     2941 agaaccgttc cgactttgat gaaaacgatc actgtgttca cgtccgccga ttcttcaatc
     3001 ccgatgatca gcagagccgt gacggcgaga atgaccagga acgcgacgat gttgaaaata
     3061 ccagtggcgt gcgggagtcc tgcggcgctg acgcccattg gcagagtgct cgccgggagc
     3121 cacagcttgt gataaagaac caggggcgtg ccgggagtcg ccgtcaattg cggaggaata
     3181 tagatgccca gacgttccag tagctgcaca acattcgcgc tccagccgga agcgacggtc
     3241 gcggcgccga acgcgtattc aaggatcaga tcccacccga tgatccatgc gaagatttcg
     3301 ccgagcgtcg cgtagccata ggtgtaagcc gaaccggcga tcggaatcag cgacgcgaat
     3361 tccgcgtagc acagtccggc gaacacgcag ccgataccgg cgaaaatgaa cgacaaaatg
     3421 agcgccggcc cggcgtaaag cgccgcggcg gatcccgtga gcacgaagat ccccgttccg
     3481 acgatcgctc caatgccgag cgagatgaga ttgaccggcc cgagcgtccg cttcagagta
     3541 cgctcgccgg tatccgccgc ctcggcatgg atcatctcaa gcggtttacg tgccagcagt
     3601 cctgccatgt ttaagtacgg taactggcgg agagtctcaa gcgcaagtgg cgaacgcaaa
     3661 gtagagaaat gccgtcgacg gcatcgtagg tgttttctca gttggaatcc cgggaatcca
     3721 gccacatgcc aaaatccatt gctcgcgtgg agagtcggtg cttcaacagg cgtccgtagc
     3781 gatttcgtag tatggatttg tcagggttaa caatcatggt gcgctctggg tcgcaagtgg
     3841 tgtggagtag tgtccatgcg gcgttatccg caggtggaag gctatagccg ataattgtga
     3901 tttcctcagc actcctcaac atatctgcgg ccattttcca caatctacta aaaggctggt
     3961 atggcctgtc ttctcctcca tatgctttca agaagcttgg atgcagcaat atctgggact
     4021 cgtttggcgg ccggtgtggt agcgacgcgt cgacattgtg gatgccgagt ccttgaagga
     4081 gcagcggatc aagagctatt tcggtttcca gcgggcggga gaaagtgcct ctcgcggaat
     4141 agcgccgccg ccttccgcgc tgggctgaaa ggccgggctg aaggtaggct tgctatacca
     4201 cccgatggct ccatgcaagt gcagtacctt aaccatcgag ggaggaaagt caacacgagt
     4261 tttgtcgtac tgattttgct ggaacaccaa atcagtcccg tagccatcgc cgggcgccca
     4321 cttttcctgg tcgagcagaa cccgctcgat tgttgaatca tagttgaagg tgatgacgat
     4381 atcgcctgat tccaggagct ttccaaatcg ccgaaggacc tcccatttcg gacaggcata
     4441 gctttgcttg tcgagatggt ttctgtgtaa gaaatagcct tccatcgccc agagtagttt
     4501 catgcgaacg tctctgtatt ctttgacttc agaaccgaag gagcggtgct tcgccacggc
     4561 agcttcggcc tcctcctgcg ccgcgcttgc ttttaacatg gaaatctggc tgtcggagaa
     4621 cagactttcc gctagatcta aaaccgtaaa aatctgctct atgttcccgc ttcgatatgc
     4681 ctggcatagc aatggattgg cgccgctaca caaaaactcc ttgagctttg gccagtcctc
     4741 ttggtagtgg
//
LOCUS       DATNDT010000327         3211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_405863, whole genome shotgun sequence.
ACCESSION   DATNDT010000327 DATNDT010000000
VERSION     DATNDT010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3211
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405863"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="VN788_10520"
     CDS             complement(<1..503)
                     /locus_tag="VN788_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXU21004.1"
                     /translation="MTETRRGRLTLFLGYAAGSGKTYRMLEEAQRLRAEGVDVVVGYF
                     EPHGRQDTIAKAAGLEFVPRHKVVYRDREFEEMDTRGILERKPQACLLDEFAHTDVPG
                     SEQNKRWEDALILLDAGIDVLTTMNVQHLESLNDQIWQISGIRVRETVPDWVLGKADQ
                     VVMVDVTP"
     gene            complement(505..1584)
                     /locus_tag="VN788_10525"
     CDS             complement(505..1584)
                     /locus_tag="VN788_10525"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXU21005.1"
                     /translation="MAQTQTDRPDPDQLLRQAMAEEDLDKFGRLKIFLGYASGVGKSV
                     RMLDEGRRRRVRGQDVVVGAIQPKATPEVGALLSSLETIPLKTVGSALVMDVPAILRR
                     HPQVCLVDGLAYDNPPGSPNAKRWQDVEQLLSKGISIVTSLNLQYVTERRDQVLAITG
                     KLPLESVPEEFIKSADEIVVVDAPPEMIERCAEDPVRQCQLSELRELALILAAEVVDR
                     QLEQYLRHNGVQQLWGAQERILVCVTPHINAGGLIRSARRNVERFHGELYVTYVRQSD
                     LSAEDQQALDANLALAREAGAIVEILEGEDAVDEIVRYARSQGITQIFVGHSYRQTWW
                     RRMFGDSLDDLIRAADGMDVRIFPR"
     gene            1749..2081
                     /locus_tag="VN788_10530"
     CDS             1749..2081
                     /locus_tag="VN788_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21006.1"
                     /translation="MHERKYPIAGSGQGVWSWVHVEDAAAATVASLECARGIYNIVDD
                     DPSQLSVWLPAFAAAGGAPEPPLISDEEVLRIAGPDSVYYATQLRGALNAKAKRKLAP
                     RRLEWLAR"
     gene            2066..2344
                     /locus_tag="VN788_10535"
     CDS             2066..2344
                     /locus_tag="VN788_10535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21007.1"
                     /translation="MAGEVEGFEAADYLRPHLAHRFNKASAPSNTSSVLHLFNSLSKS
                     VVAAFMMPQTSRLPPTAACIAMLVLSEQEPYSVTDNQEGFVIVGQYAE"
     gene            complement(2944..>3211)
                     /locus_tag="VN788_10540"
     CDS             complement(2944..>3211)
                     /locus_tag="VN788_10540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21008.1"
                     /translation="VAATLTGSISDVNCGAKHTAGTEADQKCVQGCVNGKGAEAVFVH
                     DGQVLKLSADSKDKVTPFLGKKVRVNGKIDGDTVTIKSIKAVKS"
BASE COUNT          694 a          927 c          896 g          694 t
ORIGIN      
        1 ggagtgacgt ccaccatcac cacttgatcc gcttttccga gaacccagtc tggcacggtt
       61 tcccgtaccc gaattccgct aatctgccag atctgatcgt tcaggctttc gagatgctga
      121 acgttcatag tggtgaggac atcgatgccc gcatcgagca ggataagagc gtcttcccaa
      181 cgtttgttct gttccgagcc ggggacgtcg gtgtgagcga attcatccaa caggcaggcc
      241 tgcggcttac gctccagaat ccccctcgtg tccatctcct cgaactcgcg atcccggtag
      301 acgaccttat gtcgaggaac gaattccaag cctgctgcct tggcaatcgt gtcctggcgg
      361 ccgtgcggct cgaaatatcc aacgacaacg tcgacaccct ccgcccggag tctctgggcc
      421 tcctcaagca tccggtaagt cttgccggag cccgcggcat agcccagaaa aagtgttaat
      481 cgcccgcggc gtgtctctgt catatcaacg ggggaagatg cgaacgtcca taccgtcggc
      541 ggcgcggatc agatcgtcga gagagtctcc gaacatgcgg cgccaccaag tctggcggta
      601 gctgtggcca acgaagatct gggtaatccc ctgggaacgc gcatagcgca cgatctcatc
      661 gacagcatct tcgccttcaa gaatttccac tatcgcgccc gcttcgcgag ccagcgcaag
      721 attggcgtcg agagcctgct ggtcttcagc ggagagatcg gattgccgga cgtaggtgac
      781 ataaagctcg ccgtgaaagc gttcgacatt gcggcgcgcg ctgcggatga ggccgcccgc
      841 gttaatgtgt ggtgtaacac agaccagaat gcgttcctgg gcgccccaga gctgctggac
      901 accgttgtgc cgcagatatt gctcgagttg ccggtccaca acctcggcgg cgaggatgag
      961 cgcgagttca cgaagctccg aaagctggca ctggcggacg ggatcttcgg cgcatcgttc
     1021 gatcatctct ggtggagcgt cgaccacgac gatctcatcc gcgctcttga tgaactcttc
     1081 gggaaccgat tcgagaggga gcttgcccgt aatggcgagc acctgatctc tgcgttcggt
     1141 cacgtattgg aggttcaaag aagtgacgat cgaaattcct tttgacagaa gctgctcgac
     1201 atcctgccag cgctttgcgt tggggctgcc gggcggatta tcgtatgcaa gcccatcgac
     1261 gagacagacc tgcgggtggc gccgcagaat cgccggtacg tccatcacca gcgccgagcc
     1321 cacggtcttg agaggaatgg tttccagcga actgagtaat gctccgactt caggggtagc
     1381 cttgggctga atagctccta ctacgacatc ctggccgcga acacggcggc ggcggccttc
     1441 atccaacata cgcacggact tacccacgcc ggatgcgtaa cccagaaaaa tcttaaggcg
     1501 gccgaactta tcgagatctt cctcggccat cgcttggcgc aagagttgat cggggtccgg
     1561 acggtctgtt tgcgtttgcg ccactatccc tatattatgg ctgacttacc gggaactctg
     1621 tgccaaacca atggtgaatt agcatcgacc tttagaaaat gggcgccaga gcgaaggcgc
     1681 tcctgctaat ggcgcttgcc gatccacggt cttctgcttc aactctgttg cgtgtgccct
     1741 gaaaacaagt gcatgaacgc aaatatccta tcgcgggttc cggccaaggg gtgtggtcgt
     1801 gggtacacgt ggaagacgcg gccgcagcga cggttgcatc gctggaatgt gctcggggta
     1861 tctacaacat tgtcgatgat gatccgtcgc aactgtcggt ttggcttccg gcatttgcag
     1921 cggctggcgg agcgccggaa ccgccgctca tcagtgacga ggaagtgcta cgaattgccg
     1981 gccccgattc ggtgtactac gccacccaat tgcgtggcgc tttgaatgca aaggcaaagc
     2041 gcaagctcgc accaaggaga ctggaatggc tggcgaggta gaaggattcg aagctgctga
     2101 ctatctccgt ccgcatctcg cgcatcgttt caacaaggct tccgcccctt caaatacatc
     2161 ttcagttctt catctattta attcgctatc caaatccgtc gtagctgctt ttatgatgcc
     2221 gcaaacaagt cgtcttcctc cgacggctgc gtgtatcgcc atgctcgtct taagtgaaca
     2281 ggagccatac tccgttaccg ataaccagga aggatttgtc atcgtagggc aatatgcaga
     2341 gtgagcgccg gtgggcgcac cattcgcgga gcttagctcg ctcatcatat tgaatgatgt
     2401 caaagttgcc cgacgtctga atcgtcatag atatcctgcg gctcaagtcc agcgcgaaag
     2461 tcttcgcacg ccagcagctc gttcaagaag caaggaattt cgggcaacgc aaaggggtga
     2521 tcggagccgt caacacatcg cctcatgtac cggagcagac aatcatgcac gtgaggaaac
     2581 ccaaatcgtc gtgaactgtc aagcggcaaa actcacagat ctgataatcc cgagtcagaa
     2641 agggtttagg aactcagatc agggcgtccc gcggggtcgg gatttgggct acagtacctc
     2701 tagtgagcct gcatttgatg ccaagcccgg cttgagtacg gagggttggc tccggaaggt
     2761 tccggtccaa acgtcctcaa atggcggagt ggccaaagcg acgccagacc gcgagacaaa
     2821 agcacgcgac ctcgagggca cgtcatccgg ctcagtcgct actcctagcg tgaaaacaca
     2881 acgcagcgca caccatctcc acatttcgtg acggcgtgcg ccgcgccttt aattttcaaa
     2941 aaactagctc tttacggctt tgatggactt aatggttacc gtatcgccat cgatcttgcc
     3001 gtttactctc accttttttc ctaggaaagg cgtgacctta tccttggagt cggccgaaag
     3061 cttcagcacc tggccatcgt gaacgaacac ggcctccgcg cctttgccgt tcacgcagcc
     3121 ttgcacgcac ttctgatcgg cttccgtgcc ggccgtatgt tttgcgccgc aattgacgtc
     3181 tgagatggaa ccagtcaatg tggccgccac c
//
LOCUS       DATNDT010000328         4154 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3514910, whole genome shotgun sequence.
ACCESSION   DATNDT010000328 DATNDT010000000
VERSION     DATNDT010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4154)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4154)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4154
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3514910"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1302)
                     /locus_tag="VN788_10545"
     CDS             complement(<1..1302)
                     /locus_tag="VN788_10545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21009.1"
                     /translation="MKDDRRFHGVALPFIFTPHPVVGMSGSVLRGYIDGKDPATNRSV
                     IDEVIDALTKPVNGGESASDFAGDVSTGSRQALLEPDTEDNLQRLFFERGWTDGLPIV
                     LPTEERVAKMLAGTSHARDEVVGEIFLIDTKERVKFTVEMVAIVAVMAGARPEHLPVI
                     LAIASTGQPSIQPSTTPFACMLLVNGPIRNEIGMNSGIGAFSPRNLANAVIGRAWTLL
                     TINWGYMKLKKTLWSSQGNNFGYNNMCCAENEERSAWEPFHVQKGYRPDESVVSLFRG
                     WTLLNNGGGASLRSYGEEMMIQMKALSGLYSAATLIMDPLVARNLKENEGFRTKLDYS
                     RWLSENFKVPAGRFWKTDLVDMLMAPFANQGVEPYATWKRLPDDAMIAPYNKPEQINI
                     IVVGGETSPLWKIADYAHSVSAAVDKWRAATSAEACSDGSCG"
     gene            complement(1402..1776)
                     /locus_tag="VN788_10550"
     CDS             complement(1402..1776)
                     /locus_tag="VN788_10550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21010.1"
                     /translation="MTADLPEPRGVDAMPTGLLSVLTPASQFTVTAERGATQSAKPVS
                     SSSLAPRLDKLNGKTVYLVDTGFGGSYKFLDEIQGWFAEHMPSVKTVRRRKTGNIFRD
                     DTKDLWTEIKDQGNAAILGVAG"
     gene            complement(1864..2361)
                     /locus_tag="VN788_10555"
     CDS             complement(1864..2361)
                     /locus_tag="VN788_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010502427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU21011.1"
                     /translation="MRAIDSILAELDQEAKITRRVLERIPEDKLSWKPHVKSFSLGQL
                     GLHIASLPGGLAAAIVNDSREVPNFARPEPKSRREILDTFSEGLESAKETLKKMDDAQ
                     LASTWSLTRNGKAVLSMPRIAFLRSILLNHSYHHRGQLSVYLRMLDVPLPSIYGPSAD
                     ENPFE"
     gene            complement(2431..2928)
                     /locus_tag="VN788_10560"
     CDS             complement(2431..2928)
                     /locus_tag="VN788_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002649358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXU21012.1"
                     /translation="MNSTAAGIAGATSKTIEDLTLEVKQETHVRAPLEVTFTALLEQL
                     GAAFSTPDQTAMPMKIEPWPGGRWYRDLGGENGHFWGHVQAIKRPTLLEITGPMFMSY
                     PVASNIQWRLSEVEGGTLITLHHSAFGLIQSNHREGVGKGWTHIEESVRKRAEASQSG
                     KTAAR"
     gene            complement(2954..3286)
                     /locus_tag="VN788_10565"
     CDS             complement(2954..3286)
                     /locus_tag="VN788_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXU21013.1"
                     /translation="MARAATTSDAFNAVAEPRRREILNYLAVEERPVGTIVAVLGLEQ
                     PSVSKHLRVLRDVGLVNVRRNGRRMLYRTNAEAIRPLHEWAGTFERFWQHQLARVKER
                     AEEKMKKT"
     gene            3558..>4154
                     /locus_tag="VN788_10570"
     CDS             3558..>4154
                     /locus_tag="VN788_10570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21014.1"
                     /translation="MGLRTKFLLSLVLTTTGLSCATLLVVRHTAQKRLQQEIVSDAQN
                     SGLTLQLLLHQREAMLNRKASLLGTLAAVTLDDDPTLQDSADNPLDTEGNDLVILANG
                     DHRITALHTTNPRFQQADAQQLVAYADREHRTVDWWYAAGTLYQVVIQHVENRTGETG
                     NKPETVVVGRELGYSAVHDLGRISASDVAFSYRDDIAAS"
BASE COUNT          858 a         1345 c         1043 g          908 t
ORIGIN      
        1 accgcagctt ccgtcgctgc aagcctcggc gctggtagcc gccctccact tgtccaccgc
       61 tgcgctcacc gagtgggcat aatccgcaat cttccacagc ggactcgttt ccccgcccac
      121 cacgatgata ttgatctgct cgggcttgtt gtacggcgcg atcatcgcat cgtccggcag
      181 ccgcttccat gtggcatatg gttcaacgcc ctggttcgca aagggcgcca tcaacatgtc
      241 aaccagatcg gtcttccaga atcgccccgc aggaaccttg aaattctcag atagccatcg
      301 gctgtaatct agcttcgtac gaaagccttc attctccttc agatttcggg ccaccagcgg
      361 gtccattatc agcgtcgccg ccgaataaag tccggagagg gctttcattt ggatcatcat
      421 ttcctcgccg tacgacctca gtgaagcccc tcctccgttg ttcaagaggg tccatcctct
      481 gaataagctg accacgctct catctgggcg gtagcctttc tgcacatgaa acggctccca
      541 cgcgcttctt tcctcgttct cggcgcaaca catattgttg tacccaaaat tattaccttg
      601 agagctccat agagttttct tcagcttcat gtagccccag ttgatcgtca gtaatgtcca
      661 cgcccttccg attacggcat tggctaaatt ccgcggacta aacgcgccaa tccccgaatt
      721 cattccaatt tcattgcgaa tgggcccgtt caccagaagc atgcacgcaa acggcgtggt
      781 tgacggctga atggaaggtt gtccagtcga cgcgatggcc aaaataaccg gcaggtgctc
      841 cggtcttgct ccggccatca ctgcgacgat cgccaccatc tccaccgtga atttcacgcg
      901 ctccttggtg tcaatcagga aaatttcgcc gaccacttca tcgcgagcat ggcttgtgcc
      961 cgcgagcatt ttcgccacgc gctcttcggt aggaagcacg atcggcaatc catcggtcca
     1021 tcccctttca aaaaacaggc gctgcaaatt gtcctcggta tccggctcga gcaacgcctg
     1081 gcgcgatccc gtggacacat ctcccgcaaa atcggacgcg ctctcaccgc cattcaccgg
     1141 cttcgtaagc gcatcgatca cttcgtcgat caccgatcga ttcgtcgccg gatctttgcc
     1201 atcgatatat ccgcgcagca cagaacccga catgcccacc accggatgcg gagtaaaaat
     1261 aaatggaagc gcaacgccgt ggaatcgccg atcgtctttc acaacctcgg cgaactcgaa
     1321 tgtcgagatg ggagcggtag gtacgtcata ctcctgctcc agggttcggg cacaagcggc
     1381 gaccgccgct gcgcagccgg gtcaaccggc cacgcccagt atcgccgcat tcccttggtc
     1441 cttaatctct gtccacagat ccttcgtgtc gtcccgaaag atgtttcccg tttttcgccg
     1501 tcgcaccgtc ttcaccgacg gcatgtgctc cgcgaaccat ccttgtatct cgtccaggaa
     1561 tttgtagctt ccgccaaatc cggtatccac cagatacacg gtttttccat ttaacttatc
     1621 cagtcgcggc gccagcgaag aggaggaaac cggcttcgcg ctctgtgtcg ctccgcgctc
     1681 cgccgttacc gtgaactgac ttgctggggt tagaactgat agcaatccgg tgggcatggc
     1741 atccactcct cgcggctcag gcaaatcagc cgtcaatcta aaattccggc ctcattatac
     1801 gcccggcccg caacgcgcac acgccaacgc aaaacttttc gaggctctcg aattttgcgc
     1861 ggactactcg aacggattct cgtccgcgct cggcccataa atcgagggca acggcacatc
     1921 cagcatccgc agatacacag aaagctgtcc gcgatggtga tagctgtgat tcaagagaat
     1981 tgaccgcagg aacgcgatcc gcggcatcga aagcacggcc ttcccgtttc tcgttaaact
     2041 ccacgtggac gccagctgcg cgtcgtccat cttcttcaac gtctccttcg cgctctcgag
     2101 accttccgag aaagtgtcca aaatttcccg ccggctcttc ggctccggcc gcgcgaagtt
     2161 cggaacttcc ctgctgtcgt tcacgatcgc cgcggccagg ccgcccggca aggacgcaat
     2221 atgcagcccc aattggccca acgaaaacga tttcacgtga ggtttccagc tgagtttgtc
     2281 ctctggaatt cgctcaagca ctcgccgcgt gatttttgct tcctgatcaa gctccgccaa
     2341 aatcgaatcg atagctctca tgtatttttc tcctttcatc taaattccgc atgaatcttc
     2401 tgcgccctgc taccgctgcc gcagcggcgc ctagcgcgcc gccgtttttc cagactgtga
     2461 cgcctcggcc ctcttccgca cgctctcctc gatgtgcgtc cagcccttcc ccactccctc
     2521 tcggtgattg ctctgaatca gcccgaacgc ggagtggtgc aaagtgatca gcgttccgcc
     2581 ttccacttcg ctcagccgcc actgaatgtt cgatgccact ggatacgaca tgaacatcgg
     2641 ccccgtgatt tcaagcagcg tgggccgctt gatggcctgc acgtgtcccc agaaatgacc
     2701 gttttcgccg ccaagatcgc gataccatct gccgcccggc cacggctcga tcttcatcgg
     2761 catggctgtt tgatcgggcg tgctgaacgc cgcgcccagt tgctccagca gcgccgtgaa
     2821 tgtcacctcc agcggtgctc gcacatgcgt ttcctgctta acctccagag tcaaatcctc
     2881 gatggttttg ctggttgctc ctgcgattcc cgctgctgtc gaattcatgt ttcctcctga
     2941 aattgaaatc gaatcaagtt ttcttcatct tctcttcggc ccgctccttc acgcgagcca
     3001 actggtgctg ccaaaaacgt tcgaaagtgc ccgcccattc gtgcaacggc cggatcgctt
     3061 ccgcgttggt tcggtaaagc atccggcggc cattgcgccg cacgttcacc aaaccaacgt
     3121 cgcgcagcac ccgaaggtgc ttcgatacgg acggctgctc cagccccagc actgccacaa
     3181 ttgtgccaac cggccgctcc tcgaccgcga ggtagttcag tatctcgcgc cgccgaggct
     3241 cggcaactgc gttgaaggcg tctgaagtgg tagcggctcg tgccatatcg aacaaaatat
     3301 tacattccca tataggaata tgtcaagccc aaaatcctag cctaccagtg gacctaaagc
     3361 cagctccggc gcgccaccga accggcgcaa caggacgcac cagtagggag aagcaaaagt
     3421 acctctaggg cggtaccact gggccatttc ctctgtagta gccgcaactg taccctcaga
     3481 gggtggagtt acggggccca gatgcgccgg gccgcttctc aatgaaaaca gtcgcgatac
     3541 tgaggaagca ctcgagcttg ggcctgcgca ccaagttcct gctctcgttg gtacttacta
     3601 ccaccgggct aagttgcgcc acgttgttgg tggtccggca cactgcccaa aagcggttgc
     3661 agcaggagat cgttagcgac gcccagaatt cggggctcac tctgcaattg ctgctccacc
     3721 aacgcgaagc aatgctgaac cgcaaggcca gcctgctcgg aacccttgcg gccgttactc
     3781 tggacgacga tccgaccctc caagattcgg ccgacaaccc cctcgatacc gaaggtaatg
     3841 atttggtgat cctggcgaat ggcgaccaca ggatcacggc cctgcacacg accaatcctc
     3901 gcttccagca ggctgacgcc cagcagttgg tcgcgtatgc ggatcgcgag catagaacgg
     3961 tggactggtg gtacgcggcc ggcactctgt accaagtcgt gattcaacac gttgaaaacc
     4021 ggacagggga gacaggcaac aagccggaaa cagtggtggt cggccgggag cttggatatt
     4081 cggcggtcca cgacctcgga cgaatttcgg caagcgacgt ggcattcagc taccgggacg
     4141 atattgccgc aagc
//
LOCUS       DATNDT010000329         4547 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_690438, whole genome shotgun sequence.
ACCESSION   DATNDT010000329 DATNDT010000000
VERSION     DATNDT010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4547)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4547)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4547
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690438"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..639)
                     /gene="accB"
                     /locus_tag="VN788_10575"
     CDS             complement(82..639)
                     /gene="accB"
                     /locus_tag="VN788_10575"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HXU21015.1"
                     /translation="MPKKQNAGQGGGVRGVDLAEVEQLLAFMEKHELEEFEYERAGVR
                     VRLKKASAGPVGRQLAAIGASGTAEAAFPLPAQASHAVSAHPASATHHDASDASAIGA
                     DVHIVKSPIVGTFYSAPGPEASPFVTVGTKVDVGQVLCIIEAMKLMNEIESDAAGEIV
                     NVFVENGKPVEYGEKLFGIRPHAKK"
     gene            complement(640..1701)
                     /locus_tag="VN788_10580"
     CDS             complement(640..1701)
                     /locus_tag="VN788_10580"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HXU21016.1"
                     /translation="MDRLRALLPGIGADVLLVSLLPNIRYLCGFTGSAALLLVESSRS
                     SLFTDSRYTLQAREEVSGTRIHIAKHGLIGAVAETLRSHKKPVRVGYSPHGLTIAQKK
                     ILDARSGTGIRWIPDGNIVEGLRAVKDADELETMRDAANLITRVFCDLIRNISTKMSE
                     IEVAAEIEYKIKRYGGSGPSFDTIVASGPRSAWPHAKPTSKLLGKSQFVVLDHGAIIR
                     GYCSDMTRTVFLGKATKAVKETYAAVLEAQAAAREALRPGVRAGVVDAAARGVLKRFK
                     LDKYFTHSTGHGLGLEVHEMPRLGQNERTIMREGMVVTVEPGVYISKHYGVRIEDEVV
                     VTAAGAVNLTTAPREFLEL"
     gene            complement(1798..4254)
                     /gene="pilQ"
                     /locus_tag="VN788_10585"
     CDS             complement(1798..4254)
                     /gene="pilQ"
                     /locus_tag="VN788_10585"
                     /inference="COORDINATES: protein motif:HMM:TIGR02515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus secretin PilQ"
                     /protein_id="HXU21017.1"
                     /translation="MRIKWWVGALGALIAASLMSTNGSLRAADAPLVHVSAAVANGGV
                     RVQAEANGPFEYTTYRPSATLYVVDLTGVSAGDPAGARVVASDLVKSYRVLSYSSGAK
                     PVVRVEILLAQGVEPTIERSDNRDLTLVVSKIANASLPTSTPAKTEPLAPGAAMVPAA
                     ARPSEENSSVEAIRRVSLAQNGNQTEVTISGSGALTYHSQRLRNPERLVLDFAGSRLA
                     TPEKHIASNLDPVRAIRLAQFSPEVSRVVIDLREPAHYAIHGSGSAVTVSFAAKSEAG
                     ASVSPASDSAPVVSTKSVAPAVTDAALESAAVMPTSIPAAAILPVSLTQSTSGLAMPA
                     ISANREQGPAAITLARNISSSPASRPNANLPTAKASASVSVPDTNARPASPQAAPRAA
                     QKFSGEPISVNLKDVDLHDFFRLIHEISGLNVVVDPSVKGTLTIVLDDVPWDQALDIV
                     LRNNDLDKQLDGNVLRIATKATLRKEAEENRDLAKAQAEAADVVTTTRVLSYAKATAM
                     ATTLKKFLSSRGDIISDDRSNTLIIRDIPSTLPVMDNLIRQLDRKSQQVEIEARVVAA
                     NRSFSREIGTQFGFAGANPSGSNIVGGTPQVGTSPVIRNFPPLPAPPIIVGSGGSSAS
                     SGSIPLASNLGAGVATSGIQYSFSSANFALDMIITAAEDRGVGKLLSKPKVITQNNEK
                     ATVKQGTKIPVSTIINNTVSVQFVDAVLELDVTPQITADGTIYMDVTVENDQIDAAIP
                     RVQGIPAIDTQAAETKVTVNDGATVVIGGIIVTQQRTDIQQVPFVGSVPLLGNLFKHT
                     TVSSTSQELLFFLTPRILPS"
     gene            complement(4251..>4547)
                     /locus_tag="VN788_10590"
     CDS             complement(4251..>4547)
                     /locus_tag="VN788_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21018.1"
                     /translation="NQRKAGGGVPLPPGKAGLVIATMRVDGAVHSPSGMIAVVSNPQN
                     SVYFIHEGDRLYDGDVEKIGLDGVTFRENSKDAFGKPVERMVTKRIYASAGEQQ"
BASE COUNT          914 a         1342 c         1308 g          983 t
ORIGIN      
        1 atttttcgga acatgacctt cgaagcgccg tcgcggaaaa ttccggcgat cgtctcaaat
       61 ttctgcgcgg ttcaggacca gctatttctt agcgtgcggc cgaatgccga atagcttttc
      121 cccatactct acgggcttgc cgttttccac aaaaacgttc acgatttcgc ccgctgcgtc
      181 ggactcgatc tcgttcatca gcttcatcgc ttcaatgatg cacagcacct gcccaacgtc
      241 caccttcgtt ccgaccgtta caaacggcga agcctcggga ccgggcgcgg aatagaacgt
      301 ccccacaatg ggagacttaa cgatgtggac atccgcgccg atggcgctcg cgtccgacgc
      361 gtcgtggtgc gtcgcggaag ctgggtgggc agaaactgca tgtgaagctt gcgcaggcag
      421 ggggaaagcc gcttcagcgg tcccggaagc gccaatagcg gccagctgac gtccaacagg
      481 tcctgccgag gccttcttca agcggacgcg aacgccggcg cgctcgtatt cgaactcctc
      541 gagctcgtgt ttctccataa atgctagcaa ctgttcgact tccgctagat cgacgccgcg
      601 gacgccgccg ccttgccctg cattctgctt ttttggcatt tagagttcga gaaattctcg
      661 cggcgctgtc gtcagattga ccgcaccggc ggctgtaacc accacttcgt cttcaattct
      721 gactccgtaa tgcttcgaga tgtaaacacc cggctcgaca gtaacaacca ttccctcgcg
      781 cattattgtg cgctcgtttt gcccaagtct tggcatttca tgcacttcca gccccagccc
      841 atgaccagtg ctatgcgtaa agtatttgtc aagtttgaag cgtttgagca cgccccgcgc
      901 cgccgcgtcc accacccccg ccctcacccc cgggcggagg gcttcccgcg ctgcagcttg
      961 ggcttccagc accgcagcgt aagtttcctt cactgctttg gtcgcttttc ctagaaaaac
     1021 ggtgcgggtc atgtcgctgc aatagccgcg aattatagca ccgtgatcca ggacgacaaa
     1081 ctggcttttc cccaacaact tcgacgttgg cttggcgtgg ggccacgcgg agcgcggccc
     1141 agaggcaaca attgtgtcga aggacggccc ggagccgccg tatcgcttga ttttgtattc
     1201 gatctcggca gccacctcga tttcactcat tttcgtactg atattgcgga tcaaatcaca
     1261 gaaaacccta gtaattagat tggctgcgtc gcgcatcgtt tccaattcat cagcatcctt
     1321 cacggctcga aggccttcga cgatgttccc gtccggaatc caacggatgc cggtgccgct
     1381 gcgcgcgtcg agaatcttct tttgggcaat ggtgaggccg tgtggcgaat atcccactct
     1441 gacgggcttc ttatgacttc ggagtgtctc agcaaccgcg ccgatcagtc catgtttagc
     1501 aatgtggata cgagttccgg aaacttcttc tcgggcctgg agggtgtagc ggctatcggt
     1561 aaagagcgag ctgcgcgatg actcgacgag caggagcgcg gcgctgccag tgaaaccgca
     1621 aaggtaccga atgttgggga gaagagaaac cagcaaaaca tccgcgccaa ttcccggaag
     1681 aagagcccgg agcctgtcca tccgatagga cgggccgctc cgggtttctg tacgcgttgt
     1741 cgacttggct ctgacgctca caaagttccg gcttggcggc cggaaccatc ataacctcta
     1801 gctcggcaaa atccgaggcg tgaggaagaa caatagttcc tgagatgtcg aactcaccgt
     1861 cgtgtgtttg aaaaggtttc cgagtagcgg aacgctgccg acaaacggga cctgctgaat
     1921 atcggttcgc tgctgggtga cgatgatgcc gccgatcaca acggtcgcac cgtcattgac
     1981 agtgactttc gtttccgccg cctgcgtatc aattgccgga attccctgaa ctcggggaat
     2041 ggccgcgtcg atttgatcgt tctcaaccgt gacgtccata tagattgtgc catccgcggt
     2101 aatctgcggc gtcacgtcca gctcgagcac ggcatcgaca aattgcactg agacagtatt
     2161 gttgataatc gtggagacgg gaattttagt tccctgcttc actgttgcct tttcattatt
     2221 ctgcgtgatc accttcggtt ttgagagcag tttgcctact ccgcgatctt ccgcggccgt
     2281 gatgatcata tccaaagcaa agttagccga ggagaacgag tattggatac cgctggttgc
     2341 cacgcccgct cccaggttgc tggccagcgg gatagatccg ctcgacgcgg atgacccgcc
     2401 ggagcccaca ataattgggg gggccggaag cggaggaaag tttcggatga cgggactggt
     2461 accgacttgg ggcgtcccgc cgacgatatt gctgccgctg gggtttgcgc cagcaaaccc
     2521 aaattgggtg ccgatttcgc gggagaagga gcgattggcc gccacgacgc gagcctcgat
     2581 ttccacctgt tgcgattttc ggtccagctg cctgatcaga ttatccatca ccggcagcgt
     2641 gctcggtatg tcgcgaataa tcagggtgtt cgagcggtca tcggagatga tatctccgcg
     2701 cgaggagagg aatttcttga gcgtggtagc catggccgtg gccttggcat agctgaggac
     2761 gcgggttgtg gttaccacat cagccgcctc cgcctgagcc ttggcaagat cgcggttctc
     2821 ctcggcttcc ttccgcagcg ttgctttggt ggcgatgcga agcacattgc catcgagctg
     2881 tttatcgaga tcattattcc gcagaacgat atccagcgcc tggtcccacg gcacatcgtc
     2941 gagaacgatg gttagcgtgc ctttcacact gggatcgaca accacgttca ggccgctgat
     3001 ttcatggatg aggcggaaaa aatcgtgcag gtccacatcc ttcagattta cggaaatcgg
     3061 ctcacctgaa aacttctggg cagctctggg cgccgcttgg ggggatgcag gacgggcatt
     3121 tgtgtcagga accgaaacgg aggctgatgc cttcgctgtt ggcaagttcg cattcggccg
     3181 actcgctgga gaagacgata tgttgcgcgc gagagtgatc gccgcgggcc cttgctctcg
     3241 attggcagaa atcgcgggca tggcgagccc tgacgtcgac tgcgtcaacg aaacaggcag
     3301 aatggcggcg gccgggatgc ttgttggcat gaccgctgca gattccagcg cggcgtccgt
     3361 aaccgccgga gcgacggact tggtgctgac aactggcgca ctgtcggagg cgggcgaaac
     3421 cgaagctccg gcttcactct ttgccgcaaa ggagacggtg acggcgcttc cgcttccgtg
     3481 aatggcatag tgagcaggct cgcgcagatc aatcactaca cgcgaaacct cggggctgaa
     3541 ctgtgccaag cgaattgccc gcacaggatc gaggttgcta gcgatgtgct tttccggcgt
     3601 ggcgagacgc gagccggcaa aatcgagaac caaccgctcg ggattccgaa gccgctggga
     3661 gtggtacgtc aaagcgcctg agccggagat ggtgacttcg gtctggttgc cattctgcgc
     3721 caaagacacg cggcggatgg cttcgacgct ggaattttct tccgaaggcc tagctgccgc
     3781 cggaaccatg gccgcccctg gggccagagg ctcggtcttc gcgggcgtac tcgttggcaa
     3841 actggcattc gcaatctttg aaaccaccag cgtgaggtcc cggttgtcgc tacgctcaat
     3901 cgtcggctcg acgccctgtg ccagaagaat ttccacgcgg acgacgggct tcgctcccga
     3961 ggaatacgag agcacgcgat agctcttcac cagatccgag gcaacaacgc gcgcaccggc
     4021 gggatcgccg gctgatacgc cggtcaggtc gaccacgtag agggtcgcgc tgggccgata
     4081 cgtggtgtac tcgaatgggc cgttggcttc ggcctggacg cggacacctc cgttcgccac
     4141 tgccgcactg acgtgcacca gtggagcgtc cgccgctcgc agcgatccat tcgttgacat
     4201 caggctagcc gcaatcagtg cgcctagcgc ccccacccac cacttgatcc tcattgctgc
     4261 tctcctgcac tggcgtagat tcgcttcgtc accatgcgct cgacgggctt gccgaatgca
     4321 tccttcgagt tttcgcgaaa cgtgactccg tccaacccga tcttttcaac atccccgtcg
     4381 taaagccgat caccctcatg aatgaaataa acactgttct gcggattcga aaccacggca
     4441 atcattcccg aaggagaatg cacggcgcca tccacgcgca tcgtggcaat cacaaggccc
     4501 gcttttccgg ggggaagagg cacaccgccg cccgctttcc tctggtt
//
LOCUS       DATNDT010000330         5360 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1509610, whole genome shotgun sequence.
ACCESSION   DATNDT010000330 DATNDT010000000
VERSION     DATNDT010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5360
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509610"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(291..3050)
                     /locus_tag="VN788_10595"
     CDS             complement(291..3050)
                     /locus_tag="VN788_10595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU21019.1"
                     /translation="MTPSTATLPGVYDQRLVVLSVLIAILAAYAALDLAGRVTAARSR
                     LRIAWLAGGATAMGIGIWSMHYIGMLAFTLPIPVRYDWPTVLVSLLAGIFASAVALFA
                     VSRRRMGHLQAVAGSIVMGSGIAAMHYIGMAAMRLDAMCHYSAALVILSIVLAIVISL
                     VALYLTFRLREDTGLGWWQKIASAVLMGLAIPVMHYTGMAAVTFTRAPGMAAMDGLSH
                     SIDVSYLGVFGISTVTLMVLGLAMVTSLADRRFSAQAFALQLSEDCFRQLVESVQVLL
                     WRRDADTSQFSFVNQEAERLLGYPAAQWMSSPAFWNDHIHPEDRDLAESCCGKAAAGE
                     QPQQFDLRMIAADGRLVWLRCSVRILPGKEHANELAGVMVDITKRKEAQEAAESANRA
                     KSQFLANMSHEIRTPLNGVIGMTSLLLDTTITEEQKEYVETIRSSGDALLIIINDILD
                     FSKIEAGKLDLEPLHFDIRTLVEEAVELVVDSAEGKGLELQIVVEDDVPTGAVGDPGR
                     LRQILLNLLSNAVKFTERGIVVVSVGQDSRTSERVVLRFAVTDSGIGISDAAQERLFK
                     SFSQADNSTTRRYGGTGLGLAISKRLAEAMGGTIGVDSRLAEGSTFWFTVDLPWSDRF
                     RTSSLPLESLSGKRVLVVDDNATNRRIARQHLIHCGMRVDEAAGGLEALSQLLAAAQE
                     KDPFALAILDFHMPLMDGLMLTRAIRSQQIGRILPLMLLASHRDRAQMVEARSLGLSN
                     YLLKPIRRANLISAVSQALGRVCAENTPASSGQRDRAKTWDARVLVAEDTPASQKVAM
                     LMLRRMGCRVDVVADGREAVEALQRMPYDLILMDCNMPEMDGFEATHAIRQLPGSRAR
                     TPIVALTANALHGDSDKCLAGGMDDYLAKPIRPECLAEKLDRWLSPSQRTETAEAEPQ
                     LTPPI"
     gene            complement(3185..3439)
                     /locus_tag="VN788_10600"
     CDS             complement(3185..3439)
                     /locus_tag="VN788_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21020.1"
                     /translation="MGHLDQSPFGLPDREMIRHDALRAGGVDPVVLLFRIVEYRKSSG
                     KLVGVPVKEAGFDAAVEMVTDLIRKNGDSRFSLEAVGFLQ"
     gene            complement(3584..4120)
                     /locus_tag="VN788_10605"
     CDS             complement(3584..4120)
                     /locus_tag="VN788_10605"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009497932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HXU21021.1"
                     /translation="MLLLGMVLVRALLMRREGIKAIKFGRIDKKDFLIPPFAIFYFYI
                     VFAAAFHFPAVSGQAFFQSGAASWAGALLCLAGLALLLLSLISLGKSFRVGIDPDHPD
                     KLVTTGVFAFSRNPIYVAFGFVLLGQFLLFSNWILLVYLVAATWLFHRQVSREEEYLR
                     AHYGRQYSAYCDRVRRYL"
     gene            complement(4224..4829)
                     /locus_tag="VN788_10610"
     CDS             complement(4224..4829)
                     /locus_tag="VN788_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HXU21022.1"
                     /translation="MKLRHTIVWAGAVFLLAALIALGLALRRVAGEIARQSVENEARP
                     ADTIVVLGAAEYRGRPSPVLEARLNHALWLYLQQMAPYIITTGGAGGDPVFTEGDVGR
                     EYLTKHGVPAEAIVVEKKGSSTVESLTAVAEIMRRMNFKSCIVVSDGYHIFRVKRMLE
                     SRGLHVYGSPRPEQPESGWRQRWLYLRQAAGYLLWRVGIPI"
     gene            4815..>5360
                     /locus_tag="VN788_10615"
     CDS             4815..>5360
                     /locus_tag="VN788_10615"
                     /inference="COORDINATES: protein motif:HMM:NF019644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HXU21023.1"
                     /translation="MTKLQQNYDYLMLTLTSARNPLLKEVRRAVQRGSLTDSGLAVAE
                     GFHLLEEALRSDCEIEVVLVAESVRTALTRHIGGLRQIRVHVAEDPLLREIAATETSQ
                     GVIALVRPPAWNIEHLFRDQSLLMILDGVQDPGNVGAILRAAEAFGATGAALLKGSVN
                     PYNPKALRASAGSVFRLPLLTG"
BASE COUNT         1191 a         1602 c         1512 g         1055 t
ORIGIN      
        1 attgataacg gcgcaacaga ccgccgcaac cgtaaggatc accagtaggg cgccggcgaa
       61 ttggcttttg aagtcccgca acgcgaatct ctgttaaggc aacgcggcta cgaaaacgct
      121 tgcagacacc tgacctgtca acaagagcaa gtttcgtgcc acctcgtccg tctggcttta
      181 tccctgagaa tactggattt tgtagcagga aacaaacgcg ttccccgtca atttcccttt
      241 tttgggagac gccactatgt gaaagtgtac agtgcggcgt ttccgatgtg tcagattgga
      301 ggcgtcagtt gcggctcggc ttccgccgtt tccgtgcgct gcgatggcga caaccaccga
      361 tccaacttct ccgccaaaca ctcagggcgt attggcttgg ctagataatc gtccatccca
      421 ccggccagac atttatcgct gtcgccgtgc agagcgttgg cggtcaacgc gacgatcggc
      481 gttcgggcac gagagccggg aagttgacgg atggcgtgag tagcctcgaa accatccatc
      541 tccggcatat tgcaatccat gagaatgagg tcgtatggca tccgttgaag ggcttcgacg
      601 gcttccctgc cgtccgccac tacatccacc cggcagccca tacgccggag catgagcatg
      661 gcgacttttt ggctcgcggg agtatcctcg gccaccagga cgcgcgcgtc ccaggtcttt
      721 gcgcggtctc tttgaccaga tgatgcgggg gtgttttcag cacaaacccg cccaagggcc
      781 tgggagacgg ccgatataag atttgcccgg cgaatgggct tgaggaggta attgctgagg
      841 ccgaggcttc gcgcctccac catctgagca cgatcgcgat gcgaagccaa cagcataagc
      901 ggcagtatcc ggccgatctg ctgggatcgg attgcccgcg tcagcataag cccgtccata
      961 agaggcatgt gaaaatcgag aatcgccagc gcaaacgggt ccttctcctg ggccgccgct
     1021 aacagttggc tgagcgcttc gagcccaccc gccgcctcat ccacccgcat gccgcagtgt
     1081 atcaagtgtt gccgggcaat ccggcggttt gtggcgttat cgtcgactac caatacacgt
     1141 tttccggaca gagattcaag aggaagagac gatgtccgaa accggtcgct ccacggcaga
     1201 tcgaccgtga accagaatgt gctgccctcc gcgaggcggc tatcgactcc aatcgttccg
     1261 cccatcgctt cggccagccg ttttgaaatg gcgagcccaa gacccgtgcc cccgtatctc
     1321 cgggtagtcg aattgtcagc ttgcgagaag ctcttgaaca ggcgctcttg tgcggcatcc
     1381 gatatcccga tgccggaatc agtgacagca aaccgaagca caacccgctc cgacgtccgg
     1441 gaatcctggc cgacggaaac gaccacgatg ccgcgttcag taaatttcac ggcattggat
     1501 agcaaattca ataaaatctg gcggaggcgg ccgggatccc ccaccgcgcc cgtgggtaca
     1561 tcgtcttcca ctacgatctg taattcgaga ccttttcctt cggcggaatc cacgacgagc
     1621 tccaccgctt cctcgactag agtccggata tcgaaatgca acggctccag atcgagcttt
     1681 ccggcttcaa ttttcgaaaa atcgaggatg tcgttgataa ttatcagcag cgcgtcgccg
     1741 gacgagcgga tagtttcgac gtattccttc tgttcttcag tgatggtggt atcgagcagc
     1801 aaagaagtca ttccgatgac tccgttcaac ggagtacgaa tctcatggct catatttgcc
     1861 agaaattgac ttttggcgcg gttagccgat tcggcggctt cctgcgcctc cttgcgcttc
     1921 gtgatgtcga ccattactcc ggcaagctca tttgcgtgtt cttttccggg aagaatacga
     1981 acggagcacc gtagccagac cagccggcca tcggcggcga tcatcctgag gtcgaattgt
     2041 tggggctgtt ctcccgcagc ggctttcccg cagcatgatt cagctaaatc gcggtcctct
     2101 ggatgaatat gatcgttcca aaacgcgggt gagctcatcc attgggccgc cgggtagccg
     2161 agaagccttt cggcctcctg attgacaaag ctgaattgtg atgtgtcagc gtcccggcgc
     2221 caaaggagga cttgcacgga ctcaaccaat tgccggaaac aatcctcgct caactgtaat
     2281 gcgaacgcct gcgcggagaa ccggcggtcc gcgagcgagg ttaccatggc taggcccagg
     2341 accattaagg tgaccgtgct gataccgaaa acgccgaggt aggaaacgtc aatggaatgc
     2401 gagagaccat ccatggcggc catgccgggc gcgcgtgtaa aggtcaccgc cgccatgccg
     2461 gtgtaatgca tcaccgggat cgccaagccc atcagcaccg cgcttgcgat tttttgccac
     2521 catcccagtc ccgtatcttc ccggaggcgg aaggtaaggt agagcgcgac cagggaaatc
     2581 acgatcgcga gtacgatcga taggatcacg agcgcggcgg aatagtggca catggcatcc
     2641 agccgcatgg ctgccatgcc gatatagtgc attgccgcga tgcccgagcc cattactatg
     2701 ctgcccgcca cggcctgaag atgccccatc ctccgccggc tgaccgcgaa cagggcgacc
     2761 gccgaagcga agatgcccgc caacagagac accaggacag tcggccagtc gtatctgacc
     2821 gggatcggga gcgtaaaggc cagcatgccg atatagtgca tcgaccagat tccgatgccc
     2881 atggcggtcg cgccgcccgc gagccacgca atcctcaacc ggctccgcgc cgcggttacc
     2941 cgtccggcca gatcaagcgc ggcataagcg gcaagaatcg cgatgagaac tgagaggacc
     3001 acgaggcgct gatcgtagac gccgggaaga gtcgcggttg aaggtgtcat tggggccgga
     3061 taggcaaagg gaggcaacgc acctgagcca tgctatctat actggtgtgc agggtttcag
     3121 gtaggtctcc cagttttttc ggcaagggca ccgtcgaaaa cccgcaggtc cgggcgggga
     3181 gctgctattg cagaaaaccc accgcttcca gagaaaaacg gctgtcgccg tttttccgga
     3241 tcaaatccgt caccatttcg accgcggcat cgaagcccgc ttccttgacc ggaacgccga
     3301 cgagcttgcc gctcgattta cggtattcga cgatccgaaa tagaagcacc accggatcga
     3361 cgccacccgc cctgagtgcg tcgtggcgga tcatttcccg gtccggcagg ccaaacggcg
     3421 attgatcgag atgccccatc cccaaattag tgtcctggac gcttgcaatg tatcactttt
     3481 ttgatgcggc taacggtgtg ttaggttcct cggagaaagg acggccaatg caatacgatt
     3541 aacaccagat agccgtggtg cacccgcgcg gcaatttgcc tgcctacaga taccttctga
     3601 ctcgatcgca atatgctgaa tattgccggc cgtaatgcgc tcttaggtat tcttcttcac
     3661 gcgacacctg gcgatggaac agccatgtgg cggcaactag atagaccaaa agaatccaat
     3721 ttgagaacag caggaattgg cccagcaaga caaacccaaa tgccacataa atgggattgc
     3781 ggctaaaagc gaaaacgccg gtcgttacaa gtttgtccgg gtggtctgga tcgattccaa
     3841 cgcgaaaact ctttccaagc gaaataaggc tcaggagtaa gagggccaat cccgccaggc
     3901 aaagaagcgc tccggcccag gaggctgctc cagactgaaa aaatgcctgc ccgcttaccg
     3961 ccggaaaatg aaatgcggca gcgaacacga tataaaagta aaaaatagcg aatggcggaa
     4021 tcaaaaaatc ttttttatct attctgccaa acttaatagc ttttatccct tctcttctca
     4081 tcagcagcgc ccggaccaat accattccaa gcagcagcaa aagagtcagc gcgccgaagt
     4141 acctttgcat aagcgttctc cgatcactcc tatataaccc cggggcgcca agaaaacaaa
     4201 aaagcgaaac gaacccaatc gccttagatc ggaatcccca cgcgccacag caggtaaccc
     4261 gccgcctgcc gcagatacag ccatctctgc cgccacccgc tttccggctg ctcgggccgg
     4321 ggcgagccat aaacgtgcaa accgcgggat tcgagcattc gcttcacacg gaagatatgg
     4381 tatccgtcgc tcaccacgat gcacgacttg aaattcatcc gccgcatgat ctcggcgacg
     4441 gcggtgaggc tctccactgt gctcgagcct tttttctcaa cgacgatcgc ttcagctggc
     4501 acgccatgtt tcgtcagata ttcccggccc acgtcgccct ccgtaaacac ggggtcgccg
     4561 cccgctccgc cggtcgtgat gatgtatggc gccatttgct ggaggtacag ccatagcgcg
     4621 tgattcaggc gcgcttcgag caccggcgac ggacggccgc gatattcggc cgcgccgagc
     4681 acgacaatcg tgtccgcggg gcgcgcttcg ttttcgacag actgccgcgc aatttcgccg
     4741 gccacgcggc gcagggcgag gcccagcgca atcagggccg cgagcaggaa gaccgccccc
     4801 gcccatacaa tcgtgtgacg aagcttcaac agaattacga ttatcttatg ctgacgctga
     4861 ccagcgcacg gaacccgttg ctgaaggagg tccgccgggc cgtccagcgc ggctcgctga
     4921 ccgacagcgg cttggcggtc gccgagggct ttcatctgct cgaagaagca ctgcgcagcg
     4981 attgcgaaat cgaagtggtg ctggtggcgg aatccgtcag aacggcgctg acgcggcata
     5041 taggaggact gcggcaaatt cgcgtgcatg ttgccgaaga cccgctgctc cgcgagattg
     5101 cggccaccga gacgagccag ggtgtgatcg cgctggtccg tcctccggcc tggaatatcg
     5161 agcacctgtt tcgcgaccag tccctgctga tgattctcga tggcgtgcag gaccccggca
     5221 atgtgggcgc tattcttcgc gcggcggaag cgttcggagc aaccggcgcc gcgctgctga
     5281 aaggttcggt gaatccatat aatccgaagg ctctccgcgc ttcggctggt tcggtgttcc
     5341 ggttgcctct gctcaccggc
//
LOCUS       DATNDT010000331         7969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1550109, whole genome shotgun sequence.
ACCESSION   DATNDT010000331 DATNDT010000000
VERSION     DATNDT010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7969
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1550109"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VN788_10620"
     CDS             <1..774
                     /locus_tag="VN788_10620"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HXU21024.1"
                     /translation="FVDAATEEGIIEQHDARLIEQVVEFGDKRVREVMTPRPDVIAIS
                     ARATLEEFRERVVETKFSRLPVHEKSLDDIVGVAMAREMLQVSEQDALHRTVREIMRP
                     PLFVPEMKFGSELLKEMQRTNQQMAIAIDEYGLVSGVVTVEDLVEEIVGEIGEEDRKS
                     VPDVIRETGGAIVLRGSVAVDKIGELFGVRLITAAQHSNATTVAGLLNGVAGHVPRTG
                     EVIEFDGLRFEVLEANQRKVLRVRARRAAAESAATAHSV"
     gene            828..1868
                     /locus_tag="VN788_10625"
     CDS             828..1868
                     /locus_tag="VN788_10625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HXU21025.1"
                     /translation="MSLLPTSLVGSYPQPDWLIDRQKLAHRMPPRVRASELWRVAPEW
                     LEQAQNDATLLAIRQQELAGLDIITDGEMRRESYSNRFATALDGVDLENPGKIISRSG
                     NETSVPRVVGKIRRKYPVEVRDLEFLRANTSRPIKITVPGPFTMSQQAQNDYYDTEEE
                     MALDYAAAVNAEMKDLFAAGADIVQIDEPWMEAHPDKARRYGLAALRRAVEGIGGTTA
                     LHICFGYAALVPGRPAAYSFLEELADSPVRQISIETAQSSLDCSVLAKLPRKTIILGV
                     LDLSTHQVETPQTVAARIKRALPFVPSDRMVLAPDCGMKYLPADVAFGKLSALAAGAK
                     LVRAEIHRRAAN"
     gene            complement(1909..2421)
                     /locus_tag="VN788_10630"
     CDS             complement(1909..2421)
                     /locus_tag="VN788_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21026.1"
                     /translation="MKVIFRFQQHQSRIRIPGFFLFGAILFCASSTFSQARNLGPKDT
                     FAAAGLTAKEIREITDGVEQSAYDTPDSWTSELRVRRVDLGSVSGMVVQGTKLLCGGT
                     GNCQTWVFRKAAGKWVSMFADQAPLADSFQFGPTSTGGVKDFIITTNSSAEASQRVIY
                     KFDGKLYRTK"
     gene            complement(2440..3363)
                     /locus_tag="VN788_10635"
     CDS             complement(2440..3363)
                     /locus_tag="VN788_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007434764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0018576 - catechol 1,2-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0009712 - catechol-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dioxygenase"
                     /protein_id="HXU21027.1"
                     /translation="MRAIDENTITDAALEQMSSTPNPRLREIMASLVRHLHTFAREVD
                     LTPGEWLEGIRFLTAVGQKCTEHRQEFILLSDTLGLSALTNALHDKRATAAATKSSLL
                     GPFYRQDAPMMKLGDSIISETEGTEVVFYGQVIDSNGRGIPNALVQVWEPDETGNYDL
                     QKHDPSVMDLRGCFRADEQGRYYFRGLCPTGYMIPMDGPVGDMIRAQKRHGYRPAHIH
                     FVIGAPGYRELVTALYVDGDAHIDSDTVFGVDQSLVIKLNKTDRSSPLPDLPSIHYDF
                     RLAVAKDQMTGRVGADPSQISGKATGSVASH"
     gene            3554..3898
                     /locus_tag="VN788_10640"
     CDS             3554..3898
                     /locus_tag="VN788_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU21028.1"
                     /translation="MGFGVGTRQFGDVTVIDIRGRFTLIEGEAIHDLLMDLFREGRKK
                     VLLNLQGVTYLDSSGIGQLVRGFCTANKNGAELKAVDLSSRAQEVLQLTNLHTVLADF
                     PDEQGALRSFFA"
     gene            complement(4464..6389)
                     /gene="selB"
                     /locus_tag="VN788_10645"
     CDS             complement(4464..6389)
                     /gene="selB"
                     /locus_tag="VN788_10645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HXU21029.1"
                     /translation="MAGARHFILATAGHVDHGKSALVKALTGTDPDRLPEEQLRGITI
                     DLGFAHLELPAAAGESSIVLGIVDVPGHEDFVKNMVAGVGSIDLSLLVVAADDSWMPQ
                     TEEHLQILTYLGVKRAVVGLTKIDLAQDEQGAITAIREKLKDTPFADAPIVPTSVVTG
                     RGIGDVKAALAQVLADAPPPADIGKPRLPVDRVFKLQGIGTVVTGTLSGGMLRRGQTA
                     VIQPSAKTARIRSIQSHNRDLETSGPGSRTALNLSLDDVEDVHRGDVITLTEFGGPSS
                     IVDVLLEISARALRPIKDGARVHVHHGSGNTAARIAFHDRKDLAPGERALAQLRMETP
                     AFVFAGDRFVLRDWSEHSTLGGGIVLDPDASQRLFHSETRARFLSPRAQAPDDAGSFV
                     ASQLARDGAAKKSQMLRKSRFSADAVAAVVSRLQAEGNLVVAGEMVIEWARWKKVQQR
                     AIDAIDARHRAHPEQAGLPLTDLRAALELDAPVAEIFDAVVADLCAREFSRSGNAIRR
                     VAHRPALSPQLQSASARIRTVLAAKPFDPPSRKDVAPDVASQQALRFLIESGEVFEVS
                     SEIVLAAESAKRATEMVREFIRSHGPATVSELRQMLGNSRRVALPLLERLDRDGVTLR
                     QGDKRSLRQPVPQKIQR"
     gene            complement(6454..>7969)
                     /gene="selA"
                     /locus_tag="VN788_10650"
     CDS             complement(6454..>7969)
                     /gene="selA"
                     /locus_tag="VN788_10650"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HXU21030.1"
                     /translation="DCACRETANTSSCMNASRLRAIPSVEKVLQSLGDAGLPRPLILD
                     QVRQELGALRKQKTIPQFDAVVSAIRARLRNLSAARIQPVINGTGILIHTNFGRSPLG
                     PAVIEKLNAIGSSYNNLEYDLTGGTRGGRASYLERALSLLCFSEAATVVNNNAAALVL
                     ILRHFCAGPEAATGRSARKGSREIPARKNEVIISRGELIQIGGGFRIPDILESSGAKL
                     REVGTTNHTTIGDYARAITRETALILKVHRSNFFMGGFVESPLPQEVAALARKKRVPF
                     VEDLGSGAIVETEAIAGVEHEPTPAEVIRRGVDLVCFSGDKLLGGPQAGIIAGKAALI
                     SALKRNPLFRALRCDKLILSALETTADIYLGSGGADFSHSAEEKSGVSDEAMRATTCI
                     PVLEMLNTPLDALRSRADKIISALASLPLTASIGAGQAQIGGGTLPQSVIPSVTIDLS
                     HKTLKPQDLATRLREHAVPVVGYIARGKLKLDLRTIFPRQDAQLASAIRVVAAD"
BASE COUNT         1638 a         2283 c         2373 g         1675 t
ORIGIN      
        1 ttcgtggacg ccgccacgga agaaggaatc atcgaacaac acgacgcccg gttgatcgag
       61 caggtggttg agttcggcga caaacgcgtg cgcgaggtga tgacgccgcg tcccgatgtg
      121 atcgccattt ccgccagggc cacactcgag gaatttcgcg aacgcgtggt ggagacgaag
      181 ttttcacggc tgccggttca cgaaaaatcg ctcgatgaca ttgtcggggt cgccatggcg
      241 cgcgagatgc ttcaagtttc tgaacaggac gccctgcacc gcaccgttcg cgagatcatg
      301 cggccgccac tctttgtgcc ggagatgaag ttcggctccg agctgctgaa ggaaatgcag
      361 cgcaccaatc agcagatggc catcgcgatc gacgagtacg gcctggtctc aggcgtggtt
      421 acggtggaag acctggtcga ggagatcgtg ggagaaatcg gcgaggaaga tcgaaaatcg
      481 gtgccggacg taatccgcga aacggggggc gcaatagttc tgcgcggcag cgtagccgtg
      541 gataagatcg gcgaactctt tggcgttcga ttgattacgg cggcgcaaca ctcgaatgcg
      601 acgacagtgg cgggattgct gaacggagtt gccgggcacg tgccacgcac cggcgaggtg
      661 attgaattcg acgggctgcg atttgaagtt ttagaagcta accagcgaaa ggtgctgcgc
      721 gtgcgcgcgc gccgcgccgc ggctgaatcg gcagccactg cccactcagt ctgacacagc
      781 gaaaccgctt gacaagtgcg gcgttcaaat cccacagttg agtccccatg agcttgcttc
      841 ctacgtcgct ggtcggatct tacccgcagc cggattggct gatcgaccgg cagaaactgg
      901 cgcaccggat gcctccgcga gtgcgcgcat cggaattatg gcgcgtggcg ccagagtggc
      961 tcgagcaagc gcaaaacgat gccacgctgc tcgccattcg gcaacaagag ctggccgggt
     1021 tggacatcat taccgatggc gagatgcggc gcgaaagtta ttcgaatcgt tttgcgacgg
     1081 cgctcgatgg agtggacctg gaaaatcccg gcaaaatcat cagccgcagt ggaaacgaaa
     1141 cctccgttcc gcgcgtggta ggcaagattc gccggaaata tccggtggaa gttcgagacc
     1201 tggaatttct gcgcgcgaat acttctcgcc cgatcaagat tacagtacct gggcctttca
     1261 cgatgtcgca gcaggcgcag aacgattact acgacacgga agaggaaatg gctctcgatt
     1321 acgccgccgc cgtgaacgcc gagatgaagg atcttttcgc cgccggggcg gacatcgtgc
     1381 aaatcgacga gccctggatg gaagcacatc ccgacaaggc gcgccgttac ggtttggctg
     1441 cccttcgaag agcggtggag ggcatcggcg gaactaccgc gctgcacatc tgctttggct
     1501 acgcggctct ggtgccggga cgcccggccg cttattcgtt tctggaagaa ttggcggact
     1561 cgcccgttcg acagatttca atcgagacgg cgcagtcgtc gcttgattgc agcgtgctgg
     1621 cgaagctgcc taggaagacg atcattttgg gcgtgctcga tctatccacg catcaagtgg
     1681 agactccgca gacggtggcc gcacgaatca agcgcgcgtt gccatttgtt ccgtcagacc
     1741 ggatggtgct tgcgcccgat tgcggaatga agtatctgcc ggcggatgtg gcgttcggaa
     1801 agctcagcgc gctcgcggcc ggcgcgaaat tagtgcgcgc ggaaattcac cgccgcgccg
     1861 cgaattgaac gcgtcttcgg ataattcgcg cattcatacc ccgcgatcct attttgttcg
     1921 atacagcttg ccatcgaact tgtaaatgac gcgctggctg gcctcggcgc ttgaattcgt
     1981 cgtgatgatg aaatccttga cgccgcccgt ggaagtggga ccgaattgaa agctatcggc
     2041 taacggcgct tgatccgcaa acatcgatac ccacttgcct gccgctttgc ggaaaaccca
     2101 ggtctggcaa tttccggtcc caccacaaag tagctttgtt ccctgaacca ccattccaga
     2161 gacactgcct aggtccactc gcctgacgcg caactcgctc gtccacgagt cgggagtgtc
     2221 ataggccgat tgctccacgc cgtccgtaat ttcgcggatc tctttcgcgg tgaggccagc
     2281 ggcagcgaat gtgtctttgg gaccgaggtt gcgcgcctgg gagaacgtcg aactcgcgca
     2341 gaaaaggatt gcgccgaata ggaagaaacc aggaattctt attcgagatt gatgctgctg
     2401 aaatcgaaaa ataactttca cctccgacga gccgatgcgt cagtgcgatg ccacgcttcc
     2461 tgtagctttg ccgctgatct gcgagggatc cgcgcctaca cgtcccgtca tttggtcctt
     2521 tgccaccgcg agccggaagt cgtaatggat acttggcagg tccggcagcg gcgacgatcg
     2581 gtcggtcttg ttcagcttga tgaccagcga ttgatcgacg ccaaacaccg tatcggaatc
     2641 tatgtgcgca tcgccgtcca cgtaaagcgc cgtcaccagt tcgcgataac ccggggcgcc
     2701 gatcacgaaa tgaatgtgtg cggggcgata gccatgccgc ttttgcgcgc gaatcatgtc
     2761 gccaacgggc ccgtccatcg gaatcatgta accggtgggg cagaggccgc gaaaataata
     2821 gcgtccttgc tcatcggcgc gaaaacatcc gcgaagatcc atcaccgagg gatcgtgttt
     2881 ctgcaaatcg taattgccgg tttcgtccgg ctcccagact tgcaccagcg cgttgggaat
     2941 gccgcggcca ttcgaatcga tcacttgccc gtaaaataca acttcggtcc cttcggtttc
     3001 ggagatgatg gaatcgccga gcttcatcat gggcgcatcc tggcgataga acgggccgag
     3061 caagctggat ttcgtggcag ccgcggtggc gcgcttgtcg tgcaaggcgt tggtgagcgc
     3121 gctgagaccc agggtgtccg agagcagaat gaactcctgg cgatgctcgg tgcatttctg
     3181 gccgacggcg gtgaggaagc gaattccttc gagccattct cccggcgtga ggtccacttc
     3241 gcgagcaaaa gtgtgcaagt ggcgcaccaa ggacgccatg atctcccgca ggcgcggatt
     3301 gggagtcgat gacatttgct cgagcgctgc atccgtaatc gtgttttcgt cgatggctct
     3361 catgatgcct ccccgttgga cgcgtatcat tctggtggcc gcgcccgctc attcggacga
     3421 gcgccatcat aaactttttc cgggaaagtt tccccttttc tgttggccgc gggatcgccg
     3481 gcgagatcgg cgcagcctgg gcttccgatt ccaagccgcg tagattagaa tgccggtccg
     3541 gaggaaggcg gccttgggat ttggcgttgg tactcgtcag tttggcgacg tgacggtaat
     3601 cgatattcgg ggccgcttca ccttgattga aggagaggcg atccatgact tattgatgga
     3661 ccttttccgc gaaggacgga aaaaagtgct gctcaacctt caaggggtta cctacctcga
     3721 tagctcgggc atcggacaac tggtgcgcgg gttctgcaca gcgaataaga acggcgctga
     3781 gctgaaggca gttgacctaa gctctcgagc tcaggaagtt ctgcagctta caaacttgca
     3841 cacggttctt gccgatttcc ccgacgaaca gggcgctctc cgcagcttct tcgcatagtc
     3901 tggcgtgtga acgcagctcc gcccggcggg gggaactgca ctcgtaaact agctaagggt
     3961 ttatgcggcg gcgagatact gccaaatgtt gcccgtatat tttaattgga agacaccacc
     4021 ctcatcagcg tgaaggaaat agtctccctt gggtatgcgg tgcgcgctcg aagaacggac
     4081 agagcagcgc gcccatcggg ccgggctgat acctcggccg tgcttgcgtg gctcggaaac
     4141 gacgtgaatt ccgtaattta cacggtactc gacaccggtt ggccgtgtcg gtaccaggcg
     4201 accggttcct ttgcgttttt ggctcatgga attacgatgc agtataccat cctatggttt
     4261 ctcgtagttc acaatattcg catcggtgct gcgattctcg taaactactg tagattaaag
     4321 acttaacaac aggacgaggc ggcgcgttgt gatttggtcg caagcgtgcc agcaatttag
     4381 cgcaggaatc gcggggaaaa gcccgttttc aagatgacgt cggcttcgag ttcggccgac
     4441 gctgttcgtg ttggtgtgtc ggatcaccgt tgtatcttct gcgggaccgg ctgacgcaga
     4501 gaccgcttgt cgccctgacg tagcgtgacg ccgtcgcggt ccaagcgttc cagcaggggt
     4561 agcgcgacgc ggcggctgtt accgagcatc tgccgcaatt cgctcacggt ggccggtccg
     4621 tgggatcgga tgaattcgcg aaccatttcg gtggcgcgct tggcgctctc ggccgccagc
     4681 acgatttcgg aactcacttc gaagacctct ccactttcaa tcaggaatcg caatgcttgc
     4741 tgcgatgcaa cgtccggcgc cacatctttt cgcgacggcg gatcgaatgg cttggcagcg
     4801 agcacggtgc ggattcgcgc actggccgat tgaagttgcg gcgacaaagc cgggcgatgg
     4861 gccactctgc gaatcgcatt tcccgaacga gagaattcgc gcgcgcacaa atccgcgacg
     4921 accgcatcga atatttcggc gacgggcgca tccaattcga gcgccgcgcg caaatccgtg
     4981 agaggcaagc cagcctgctc ggggtgcgcc cgatggcgcg catcgattgc atcgatggcg
     5041 cgctgctgga cttttttcca gcgggcccac tcgatcacca tttctccggc aaccacgagg
     5101 ttgccttccg cttgaagccg cgaaaccaca gcggcgacgg cgtccgcact gaatcgcgat
     5161 tttcgaagca tctgagattt tttcgcagcg ccatctcgcg ctaattgcga agccacgaaa
     5221 cttccggcat cgtcgggcgc ttgggcgcga ggagaaagaa atcgcgcccg agtctcgctg
     5281 tggaacagcc gctggctcgc gtccggatcg agaacaattc ctccacctag cgtgctgtgt
     5341 tctgaccaat cccgcaagac gaaccggtct ccagcgaaca caaatgccgg cgtttccatg
     5401 cgcaattggg ccagcgcgcg ttcgcccggc gccaaatctt tgcgatcgtg aaaggcaatt
     5461 cgagcggcgg tgttaccact accatggtga acgtgcacgc gcgcgccatc ttttatggga
     5521 cgcagggccc tcgcggaaat ctcgaggagc acgtcgacaa tgctgctcgg tcctccgaat
     5581 tcggtcaggg tgatcacatc gccgcgatga acatcctcga catcatcgag agaaagattg
     5641 agcgcggtgc gcgagcccgg cccgctggtt tccaaatcgc ggttgtggga ttggatactg
     5701 cgaatgcgag ccgtcttggc cgagggctga atcacggcgg tttgcccgcg acgcaacatt
     5761 cctccgctca gcgtgccagt gaccaccgtg ccgatgcctt gcaatttaaa tacgcggtcg
     5821 acgggaagac gtggcttgcc gatatccgcc ggaggcggcg catccgcgag cacttgcgca
     5881 agagcggcct tcacgtcgcc aattcctcgc cctgtgacaa cagatgtggg gacgattggc
     5941 gcatccgcaa acggcgtgtc cttcaatttt tcgcgaatcg cggttattgc tccttgttcg
     6001 tcctgcgcta aatcaatttt ggtcaatccc acgactgcgc gcttcacgcc gagataggtg
     6061 aggatttgca agtgctcttc ggtctgaggc atccagctat cgtcggccgc gacaaccaag
     6121 agcgaaagat caatcgaacc gacacccgcc accatatttt tcacgaaatc ttcgtgtccc
     6181 ggcacgtcga caattccgag aacgatcgaa gattcgccgg ccgccgccgg tagctcgaga
     6241 tgcgcgaaac cgagatcgat tgtgattcct ctcagttgtt cttcgggcaa gcggtcggga
     6301 tcggtgccag taagcgcttt gaccagagcg ctcttgccgt ggtccacgtg tcccgcagtg
     6361 gctaatatga aatgccgcgc gcctgccatt ggcgaatcat ttctccttca gacatttacg
     6421 cgaacgaagc tgctcccgac tttcgtcaaa cgcttagtcg gccgccacta ccctgattgc
     6481 actggccaat tgcgcatcct ggcgagggaa aatcgtccgc aagtccaact tcagtttgcc
     6541 gcgcgcgata tatccgacga ccggcacggc atgttccctc aaccttgtgg cgagatcctg
     6601 cggtttaagc gttttgtggg agagatcgat cgtcaccgag gggatcacgg attgcggcag
     6661 cgtccctccg ccgatttgcg cctgcccagc gccgatactg gcggtcaacg gaaggctcgc
     6721 aagcgcggaa atgattttat cggcacgcga acgcaaggca tcgagcggcg tgttcagcat
     6781 ctccagcacc ggaatgcatg tcgtagctcg catggcttca tcggaaaccc cggatttctc
     6841 ttcggcgctg tgagaaaaat ccgcgcctcc agaacctaag taaatatcgg cagtcgtttc
     6901 cagcgcggat aaaatcagtt tgtcgcagcg gagcgcgcga aatagaggat tgcgcttgag
     6961 cgcgctaatg agcgcggctt ttccggcaat gatcccagcc tgcggccctc cgagcagctt
     7021 gtctccgctg aaacagacca aatcgacgcc acggcgtatc acttcggcag gagtaggttc
     7081 gtgttccact cccgctatcg cttcggtttc aacaattgcg ccgctgccaa gatcttcgac
     7141 aaagggaacc cgcttttttc gcgccaacgc cgcaacttcc tgcgggagtg gcgattcgac
     7201 aaaaccaccc atgaaaaaat tgctgcggtg gactttcagg atcaacgcag tttcgcgtgt
     7261 gatcgcgcgg gcataatcgc caattgtcgt atggtttgtt gtgcccactt cgcgcagctt
     7321 tgcgccgctg gattcgagga tgtccggaat gcggaagccg cctccgatct gaatgagttc
     7381 gccgcgagaa atgatcactt cattcttgcg cgctggaatt tcacgcgagc ctttgcgggc
     7441 actccgccct gtagcggcct cgggccctgc gcaaaagtgg cgcaaaatca aaaccagcgc
     7501 ggcggcattg ttgttcacca ccgttgcggc ttcagagaaa cacaaaagcg atagcgcccg
     7561 ctcaagatac gaggcgcgtc cgccgcgcgt ccctccggta aggtcgtact cgagattgtt
     7621 gtagctggat cctattgcgt tcagcttttc gatcaccgcc ggcccgagtg gcgagcgtcc
     7681 aaaattcgtg tgtatcaaaa ttcccgtgcc gttaattaca ggctgaatgc gggcggcgga
     7741 taaattgcgg agtctcgcgc ggatcgccga cactacggcg tcaaattggg ggatcgtctt
     7801 ctgtttgcgg agcgcgccta attcctggcg tacctggtcg aggatcagcg gacgaggaag
     7861 cccggcatcg ccgagcgatt gcaagacctt ctcgacagat ggtatggccc ggagccgaga
     7921 ggcattcatg cacgaactcg tattcgcggt ttcgcggcac gcacaatcc
//
LOCUS       DATNDT010000332         8073 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_395918, whole genome shotgun sequence.
ACCESSION   DATNDT010000332 DATNDT010000000
VERSION     DATNDT010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8073
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_395918"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VN788_10655"
     CDS             <1..217
                     /locus_tag="VN788_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21031.1"
                     /translation="LAAAAARPDSNQFLLLVGGIGVHRSLILIWTERDPRARCREVSQ
                     KTGKRILGETGMATALMEIRTCADVHY"
     gene            267..701
                     /locus_tag="VN788_10660"
     CDS             267..701
                     /locus_tag="VN788_10660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU21032.1"
                     /translation="MPGLELIRDNSPSEARRGLRILVVDDNAINQNLAVLLVKKQGHS
                     AVVANNGREALELLEKEVFDLVLMDVQMPEIDGVEATMRLREKEQQKGLGHLPVIALT
                     AHAMAGDRERCLAAGMDGYVTKPVRVKELFAAIDDIAARLIP"
     gene            798..2456
                     /locus_tag="VN788_10665"
     CDS             798..2456
                     /locus_tag="VN788_10665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HXU21033.1"
                     /translation="MIKLITPILLSIFAAGMTFGGQFKVAKELRAVDPASTVNVIVQF
                     NHVPTMRDHERILSRGGALKGDLASGKFGAYAIPARSLSRLENDPAVLHVSANHKVHA
                     MLDLTADAVNASASWAAGWTGAGIGVAVIDSGISKHADLDSRVVYSQDFVGGGTSDLY
                     GHGEHVAGIIGGSGRTSNCNNCTRLLKGMAPGVNLLDLRVLDQNGDGNDSGVIAAIAK
                     AIELKTQYNIRVINLSLGRPVSESYMQDPLCQEVEAAWRAGIVVVVAAGNGGRDNSQG
                     TDGYATITAPGNDPYVITVGAMKTMGTPTRSDDLIASYSSKGPTLIDHVVKPDIVAPG
                     NQIVSLMAAMSQFSKTYPANIVAFSYYRNTSQNTASNQYFTLSGTSMATPVVSGAVAD
                     LLQAEPGLTPDQVKAKLMLTAYKAFPATSSAVDPATGIVYTSQYDVFTVGAGYLDIQA
                     LLNDQNVPQGTALSPTAAYDAASNNVYLITDQQAVWGTQGVWGTQAVWGTQAVWGTQG
                     VWGTQAVWGTSTVAGFQAVWGTQAVWGTSAKDGSEADAITISGE"
     gene            2606..7168
                     /locus_tag="VN788_10670"
     CDS             2606..7168
                     /locus_tag="VN788_10670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXU21034.1"
                     /translation="MKSIVVMFEKQCRPAVVWKVFLLGMLLCSTAPGIGLDPSKAITQ
                     YVHDVWLADSGLPQNSVLAIAQTPDGYLWLGTGEGVVRFDGIKFTTFDKRNTPGLHSN
                     EISSLLTGRDGKLWIGTRGGGLTVYSGGKFRSYTVRDGLSSDTVLCLFEDRSGDLWIG
                     TDGGGLNRFQNGRFSAYTTRTGLANDTVFSVAAGKDDGLWIGTHTGLSYLKSGKFRNY
                     TVKDGLADDYIRSTYVDRAGNVWAGTNRGGLSRITRDGIVNYSKKDGLSSNAVCSIFE
                     DDLGTLWLGTGAGGLDRYRNGVFSSFTSKDGLSSDEVSALFEDREGSFWIGTADGGLN
                     RLKNGAFTMYGLHEGLSSQVILPVYQDHTGAVWVGTNGGGVDRIQGGRITAYTSNDGL
                     SDDVVFSIAEDGAGDMWFGTRRGLTRFRNGRSKVFTVKDGLPDDTISSLFQDRHGNLW
                     VGTHGGLSRLEGARFITYSTRDGLSNDSVLSIFEDAAGALWIGTGGGGLNRFEDGKFT
                     AYTTANGLLNDFIWAIHGDPDGTLWLGTNGGGLERFRDGKVVSYTTGNGLFDDAVFQI
                     LDDGQGYLWMSCNKGIFRVSRQQLNDFADHRVQAIESQPFGVPDGLKVREANGGYQPA
                     GWKTADGRLWFPTIRGVAVVDPSRVRPNPVPPPVVIEKLLVDRQAAAPGSPLEFPPGK
                     GRLEFQYTALSLRAPRRLRFKYILDGFDREWTDTGNRRDVIYTNIPPGEYRFRVIACN
                     EDGVWNKTGASLSFILKPHFYQSRLFGGFCLLAVSALAAAAYRVRVKRLKAREAQLGF
                     LVYKRTQALTESERRFRQLAENIHEIFWMIEPESGRFLYISPAFEEIWGASSKTVLDD
                     ASYWFDAVHPDDRERVRESKLRQQKGERETCEYRVIRSDESVRWVWDRSFPVFNKRSE
                     LDRVVGIAEDISARKQGEEMLRNSRDELEIRVLERTGDLRRANDALQEENLERKRAEH
                     QLRGAKEAAESANRAKSEFLANMSHEVRTPMNGIMGMTELALDTDLTAEQREYLELVK
                     QSGDALLRVIDDILDFSRIEARKLHLECVEMDLRRCVEDTVKSLAMRAHEKGVELVCR
                     FAPDVPETLAGDAGRLRQILVNLAGNAIKFTRDGEILISVEVGEVAEEQVALHFSVRD
                     TGIGIPKDKQHLIFEAFTQADGSSTRQYGGTGLGLAISSQLVALMGGRIWVDSEVGQG
                     STFHFTARFARPPKSGKEAPPRHHRLGGVPVLVVEDHATNRRVIEEVLINWGAAPTCV
                     ESGHAALLALARATSGGNPYRLLISDNDLADRDDSALLKDVKQSEAFGDTPVILLTSC
                     GTAENLRWRELGVTASVTKPAAQSDLREALDGALASHQQPGNSAGVFGGSQTGASQIP
                     ASADRLQILLVEDNRTNQQVALHLLRRFGHEIVIANNGEEAIEALKGSQQPFDLALMD
                     VQMPGKGGFETTAEIRQMERGTANHLPIVAMTAHAMKGDRERCLAAGMDDYLSKPINA
                     KDLLNVIEMNVALSRGTPVEAESPAG"
     gene            complement(7165..7836)
                     /locus_tag="VN788_10675"
     CDS             complement(7165..7836)
                     /locus_tag="VN788_10675"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU21035.1"
                     /translation="MRAFRNKGLLVSGMIVMQLVGASSLFADFFAIPNPSAPYIQSTV
                     LIDFAADGNLVSSVSDGTLTISYDTDLEQAGVPAVWQTWNTPPAVETATPNVGMTDFV
                     SSLTSSFSSPVYVFGFEAEPDFLGFDEITASFYNGSTLVGTIDQNVNGTDGALLFAAL
                     TTTSPFTSVAVNDVDGDDFAIARQRYSLVSTKFSSVPEPGGLCLLGSGLLVLGFFKRA
                     PGAAD"
     gene            7958..>8073
                     /locus_tag="VN788_10680"
     CDS             7958..>8073
                     /locus_tag="VN788_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018424102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HXU21036.1"
                     /translation="MYLPAHFEEKRIEVLHELIRAHPLAALVTLGPQGLDAN"
BASE COUNT         1777 a         2140 c         2528 g         1628 t
ORIGIN      
        1 cctcgcggcc gcggcggctc gaccagattc gaatcagttt ttgttgctcg tcggtggaat
       61 cggggttcat cgcagtctca ttttgatatg gacggagcgc gatcccaggg caaggtgccg
      121 ggaagtgtct cagaaaacgg gaaaaagaat cctcggagag acgggaatgg cgacagctct
      181 aatggaaata cggacttgcg ccgatgtgca ttactaggtg gcgcgcgccg cctttgatcc
      241 gacgaaacgt gagcggcggt taaagaatgc cgggacttga attgattcgc gataactcgc
      301 cttccgaggc acggagaggt ctacggattc tcgttgtgga cgataacgcg atcaaccaaa
      361 atctggccgt tttgctggta aaaaaacaag ggcattcggc ggttgtcgcg aataatggcc
      421 gggaagcact ggagttgctc gaaaaagagg tgttcgatct ggtgctgatg gatgtgcaga
      481 tgcccgaaat agacggggtc gaggccacga tgaggctacg cgagaaagag caacagaagg
      541 gcctggggca tcttccggtg atcgccctga cggcccatgc gatggcgggc gaccgggagc
      601 ggtgcctggc agccggcatg gatggatatg tcaccaagcc ggtgcgcgtc aaagagcttt
      661 tcgcggcgat cgacgatatc gcggctcggc tgattcctta aaggaatctg gggcactgcc
      721 gaaaagcacc ctatagccgc gatttaccga aaaaagtcta gccgatcatt ccggcgagtg
      781 gctgaaggag atagtgtttg attaagttaa ttaccccgat attgctgtcc atttttgccg
      841 caggcatgac attcggagga cagttcaaag tagccaagga attacgagcc gtcgaccccg
      901 cttccaccgt caatgtgatc gtacagttca atcatgtgcc aaccatgcgc gatcatgagc
      961 gaattctaag ccgcggcggg gctttgaagg gtgatttggc atcgggcaaa ttcggagcct
     1021 acgcaatacc cgcgcgatcg ctttcacggc tggagaacga tccagcggtg ctccatgttt
     1081 cggccaatca taaggtccat gcgatgttgg atctaacggc tgacgcggtg aacgcgagcg
     1141 cctcctgggc ggcgggttgg acgggcgctg gaattggcgt ggcggtcatc gacagcggga
     1201 tctcaaaaca tgcggatctc gacagccggg tggtctactc acaggatttt gttgggggcg
     1261 gaacgagcga tctttacggc cacggagagc acgtggcggg cattatcggc ggcagtggcc
     1321 ggacttccaa ttgcaacaat tgcacccgtc tgctgaaggg catggcgccc ggcgtcaatc
     1381 tgttggacct gagggttctc gatcagaatg gcgacggcaa cgacagcggg gtaatcgccg
     1441 cgattgcgaa agcgatcgag ctgaagacgc agtacaacat tcgggtcatc aatctttcac
     1501 tcggccggcc ggtgtctgaa agctacatgc aggacccgct ctgccaggaa gtcgaggcgg
     1561 catggagagc cgggatcgtc gtcgtggtgg cggctggaaa cggaggaaga gacaattcgc
     1621 aaggaacgga cggttatgcg acgattaccg cgcctggaaa cgatccttac gtaattaccg
     1681 ttggcgcgat gaagacgatg ggaactccca cgcgctccga cgatctgatc gcgagttaca
     1741 gttccaaggg gccgacactg atcgatcacg tcgtgaagcc cgacatcgtc gctcctggaa
     1801 atcagatagt ttcattgatg gcggccatgt ctcagttcag caaaacttat ccggccaaca
     1861 tcgtggcgtt ttcgtattac aggaatacga gccagaacac ggcgtccaac cagtacttca
     1921 cgctgagtgg aaccagcatg gccacaccgg tggtgagcgg cgcggtcgcg gatttgctgc
     1981 aagctgagcc cggtctgaca cccgaccagg tgaaagccaa gctgatgctt acagcgtata
     2041 aggcttttcc ggcgactagt tcggctgtcg atccggcaac cggaatcgtc tacacgagcc
     2101 agtacgatgt tttcactgtc ggggcgggct acctggacat acaggcgctg ctcaacgatc
     2161 aaaatgtgcc gcagggcacg gctctttcgc ccaccgcggc gtatgatgcg gcctcgaata
     2221 atgtgtactt gatcaccgat cagcaggcag tctggggaac gcaaggcgtt tggggcacgc
     2281 aagctgtgtg gggtacccag gcagtctggg gaactcaagg tgtctggggt acccaagccg
     2341 tctggggcac gagcaccgtg gcaggtttcc aagcggtttg ggggacacaa gcagtctggg
     2401 gcaccagcgc gaaggacggt agcgaagccg acgcaatcac aatcagcggc gagtaaaccg
     2461 ccgacatccg aacttcacca acctaacttc actaactctg gaggagcgta gccgcacgcg
     2521 ggcagcgctt ctccgttggg tttttgcgtt cccgaatttc acgagcggcg cctaaactcc
     2581 caatccaatt gcgccgataa caaagatgaa gtcgattgtc gtcatgtttg aaaagcaatg
     2641 caggcccgcc gttgtgtgga aagttttcct cctcggcatg ctgctttgct ccaccgcgcc
     2701 cgggattggg ctcgatccat caaaagcaat tacgcagtac gtgcacgatg tctggctggc
     2761 cgattcgggt ttgccgcaga actcggtgct cgctatcgca cagacgcctg acggttatct
     2821 gtggcttggc accggggagg gggttgtgcg gttcgatggc atcaaattca ccaccttcga
     2881 taaacgcaat actccgggcc tgcatagcaa cgagatctcg tcgttactga ccggccgcga
     2941 cggcaaactg tggatcggca cgcgcggtgg cgggttgact gtttactcgg gcgggaaatt
     3001 cagaagctac accgtgcgag acggtctgtc gagcgacacc gtgctgtgtc tgtttgaaga
     3061 ccggagcggc gacctctgga tcggaaccga tggcggcggc ttgaaccggt tccaaaacgg
     3121 ccgcttctcc gcctacacga cgcgaactgg tttagcgaac gacaccgtgt tttccgtggc
     3181 ggccggtaaa gacgatggcc tgtggatcgg cactcacacc ggtttatcgt atctcaagag
     3241 cgggaaattc cggaattata cggtaaagga cgggttggcg gacgactaca tcaggtcgac
     3301 ctacgtggat cgcgccggca acgtgtgggc cggcacaaac aggggtggcc tcagccggat
     3361 tacgagggac gggattgtca attactcgaa gaaagacgga ctgtcgagta acgccgtctg
     3421 ttctattttc gaagatgacc tgggcaccct atggctcggc accggcgccg gggggctgga
     3481 tcgatatcgg aatggggttt tcagctcgtt cacctcgaag gacgggctgt cgagcgatga
     3541 ggtatcggcg ctgttcgagg atcgcgaagg cagtttctgg attggtacag ccgacggggg
     3601 actgaatcgc ctgaagaacg gcgcgtttac aatgtatggg ctgcatgagg gactctccag
     3661 ccaggttatt ctgccggttt atcaggatca tacgggcgca gtctgggtgg gaaccaacgg
     3721 gggcggggtg gaccggattc aaggcggcag gatcacggca tatacctcga atgatggcct
     3781 ctccgacgac gttgtttttt ccatcgcgga ggacggggcc ggggacatgt ggtttggaac
     3841 ccgccgcggg ctgacccggt tcaggaacgg ccggtcgaag gtctttacgg taaaagacgg
     3901 cctgcccgac gatactatct cttccctctt tcaagatcgt cacggcaatc tttgggtggg
     3961 aacgcacggg ggcctcagcc ggctggaggg ggcgcgcttc attacctatt cgactcgcga
     4021 cgggttgtcc aacgactccg tgctttccat ctttgaggac gccgccggcg cgctctggat
     4081 aggaacggga ggtggaggtc tgaaccgctt cgaggatggc aaattcacgg cctacacgac
     4141 agcgaatgga ctgttgaacg actttatttg ggcaatccat ggcgatcctg acgggacgct
     4201 ctggttgggc acgaacggcg gcggattgga acggttccgc gatgggaagg tcgtgagcta
     4261 cacaaccggc aatggccttt tcgacgacgc agtttttcag atcctcgacg acggccaggg
     4321 ttatctctgg atgagttgca acaaaggcat ctttcgcgtt tcccgacagc agctaaacga
     4381 ctttgcggat caccgggtcc aggctattga gtcgcagccg ttcggcgtcc cggatggcct
     4441 gaaagtgcgt gaggccaatg gaggatatca gcccgccggc tggaagaccg cggacggaag
     4501 actctggttt ccgacgatac gtggcgtggc ggtcgtcgat ccgagccgcg tccggccgaa
     4561 tcccgtgcct ccgccggtcg tcatcgaaaa acttctggtg gatcgccagg cggcggcgcc
     4621 gggctcgcct ttggagtttc cgccgggaaa ggggcggctg gaatttcaat acaccgcctt
     4681 gagcttgcga gccccccggc ggctccgatt caagtacatt ctcgacggat tcgatcgcga
     4741 gtggacggat acgggcaatc ggagagatgt catttacacg aatattcccc ccggagaata
     4801 tcgcttccgt gtgattgcgt gcaacgaaga tggcgtctgg aacaaaacgg gcgcctcgct
     4861 ttcgttcatt ctcaaaccgc acttttacca gagccggtta tttggcggtt tttgtctgct
     4921 tgcggtttca gccctagccg ccgcggccta ccgggttcgt gtaaagcgat taaaggcccg
     4981 ggaggcccag cttggatttc ttgtctacaa gcgtactcag gcgctcacgg aaagcgagag
     5041 aagattccgg cagttggcgg aaaacataca tgagatcttc tggatgattg aacccgagag
     5101 tgggcggttc ctatatatca gccccgcctt tgaggagata tggggcgcca gttcgaaaac
     5161 ggttttggac gacgcctctt attggttcga cgccgtccat ccagacgacc gggagcgcgt
     5221 tcgcgaatcg aagctgagac aacagaaagg tgaaagggag acttgcgaat accgggtgat
     5281 ccgctcggac gaaagcgttc gttgggtttg ggaccggtcg tttccggttt ttaataaacg
     5341 gtccgaactc gatcgcgtgg tggggatcgc ggaagatatt tccgccagaa agcagggcga
     5401 ggagatgtta cgcaattccc gcgacgaatt ggagatccgt gttcttgagc gaaccggcga
     5461 tctccggcgc gcgaacgatg ccctccaaga ggaaaacctc gagcgcaaac gcgccgagca
     5521 tcagctacgc ggggcgaagg aagcggccga atccgcgaac cgggcgaaaa gcgaatttct
     5581 cgcgaacatg agccacgaag tccgcacgcc catgaacggc attatgggta tgaccgagct
     5641 tgccctcgat accgacctaa cggctgaaca gcgggaatat ctggaactgg tcaagcagtc
     5701 aggcgatgca ttgctcaggg tgatcgacga tattctcgat ttttcccgca ttgaagcgag
     5761 gaaactccac ctggaatgtg tcgagatgga tctgcgccgt tgcgtcgagg acaccgtgaa
     5821 atcgctcgcg atgcgcgctc atgaaaaggg agtggaactc gtatgccgct tcgctccgga
     5881 tgtgcccgag accttggcgg gcgacgcggg aagactcagg cagatcctcg tcaacctcgc
     5941 cggaaacgcc atcaagttta cgcgcgacgg cgagattctc atctcggtgg aagtcgggga
     6001 agttgcggag gagcaggtcg cgcttcactt ctccgtgcga gacacgggaa tcgggattcc
     6061 aaaggacaag caacatctga tcttcgaggc cttcacgcag gcggatggat cgtcgacacg
     6121 gcagtacggg gggacggggc tgggtctcgc catttcttct cagcttgtcg cgcttatggg
     6181 aggacggatt tgggtggaca gcgaagtcgg ccagggaagc acttttcact ttacggcgcg
     6241 attcgcccgg ccgcccaaat ccggcaagga agccccgccg cgccatcata gactcggagg
     6301 cgttcccgtg ctggttgtgg aagaccacgc cactaatcgg cgggtgatcg aagaggttct
     6361 tataaactgg ggagccgcgc ccacgtgcgt cgaaagcggg catgccgcac ttctggctct
     6421 ggcgcgagct acctcgggag ggaatcccta ccggctattg atctccgaca acgatctggc
     6481 ggacagagac gactccgcgc tgctgaaaga cgtcaagcaa tcggaggcat tcggggacac
     6541 gccggttatt ctgttgacat cgtgtgggac ggcggaaaat ttgcgctggc gcgaactcgg
     6601 cgtgaccgcc tccgttacca agcccgccgc gcaatcggac ttgcgggaag cgctggatgg
     6661 cgccctcgcc agccaccaac aacccggtaa ttccgctggt gtcttcgggg gctcgcaaac
     6721 cggcgcttcg caaattcccg cgtcggctga ccgccttcag attctgcttg tggaagacaa
     6781 tcggacaaac cagcaggtcg ctttgcactt gctgcggcga ttcggccatg agatcgtgat
     6841 cgcgaataat ggcgaggaag cgatcgaggc gctgaagggt tcgcaacagc ctttcgatct
     6901 ggcgctcatg gatgtacaga tgccgggaaa gggaggtttt gaaacgacgg cggaaattcg
     6961 ccagatggag cgtgggacgg cgaaccatct tccgatcgtt gccatgaccg cccacgcgat
     7021 gaaaggcgat cgggagagat gcctggccgc ggggatggac gattatctat cgaagccgat
     7081 caatgccaag gatttgctta acgtgatcga gatgaacgtt gctctttcgc gtggaacgcc
     7141 agtggaggca gagagtccgg ccggttagtc agcggcgccg ggcgcacgtt tgaagaatcc
     7201 taggaccagc aggccactgc ccagtagaca tagaccgcca ggttcgggca cagatgaaaa
     7261 tttcgttgaa acgagagaat agcgctggcg ggcgatcgcg aaatcgtcgc cgtccacatc
     7321 gttgacggcc acgcttgtaa atggactggt cgttgtcaag gccgcgaaaa ggagagcgcc
     7381 gtcagtccca tttacgttct ggtcgatcgt ccccacgaga gtcgacccgt tgtagaaact
     7441 ggctgtaatc tcatcgaatc caagaaagtc gggctccgct tcgaagccga agacatagac
     7501 cggcgaactg aatgacgacg tcaaactgct cacgaagtcc gtcattccca cgttcggagt
     7561 agccgtctcc acggctggag gggtattcca ggtttgccaa acggcgggga caccggcttg
     7621 ctccagatcc gtgtcgtacg aaatcgtaag cgttccgtcc gacacgctgc tcacgaggtt
     7681 tccgtcagcg gcaaagtcaa tcagaacagt cgattggata tacggcgccg acgggttcgg
     7741 aatggcaaaa aagtccgcaa acaatgaact ggcgccaacc aactgcatca cgatcatccc
     7801 agagacgagc agccccttat ttcgaaacgc gcgcatagac aattctcctt atgttccaag
     7861 tgagttccgt ggatgggaga aatttctcca ggaaattaat gtgaagatct aagaaactta
     7921 cgggcgggct gcatgtcggt acgatagaaa catcccgatg tatctgccag cccattttga
     7981 ggagaaacgg atcgaagtat tgcacgagtt gatccgggcg catcctcttg ccgccctggt
     8041 gacgcttggg ccgcaaggct tggatgcgaa tca
//
LOCUS       DATNDT010000333         6872 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1510946, whole genome shotgun sequence.
ACCESSION   DATNDT010000333 DATNDT010000000
VERSION     DATNDT010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510946"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="VN788_10685"
     CDS             complement(<1..530)
                     /locus_tag="VN788_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXU21037.1"
                     /translation="MTETAAEAASPEPPRTRKGRMRAGVYRGQGRVVVEHVPIPEIRE
                     GEVLFRVAACGICGTDIKKIHHNFIPPPQILGHELAGTVVEVGRGVSKFKPGDRVVSF
                     HHIPCGACFYCKKKLFSQCAGYKKVGLTAGFDPNGGGFAEYVRAMPWIVEGGMIALPR
                     DVTFEEATFVEPVNTCL"
     gene            complement(527..1309)
                     /locus_tag="VN788_10690"
     CDS             complement(527..1309)
                     /locus_tag="VN788_10690"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21038.1"
                     /translation="MKILVTFALEAEFAPWRALREFCAGNYGAADAYVADIHGAQLAV
                     VLTGAGPRQAARQASKAIGAESDSLSACISSGLAGALQSEYSIGQVLAARSVFSEKPR
                     EDRGAQLLPSSEPLISFAADQGAKVVSRFHTAARVVSTSEEKRYLGESADAVEMESFE
                     VLRTAAACGVPAVAIRAISDVAGEDLPLGMNGIFTDEGQVSVSRVIRQVARHPQSIPG
                     LVKFGQQSKLAAESLTRFLDRYVGTLVKRTQSLESKVMTGRR"
     gene            complement(1348..2379)
                     /gene="hpnA"
                     /locus_tag="VN788_10695"
     CDS             complement(1348..2379)
                     /gene="hpnA"
                     /locus_tag="VN788_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HXU21039.1"
                     /translation="MITLVTGATGFLGSHVARQLVTAGDSVRVLVRRTSNLQALDGLA
                     AERCEGDLNDAASLKRAVAGVQHIFHVAADYRLWARNPDEIYETNVQGTRRLLEAAAN
                     AGVERFVYTSTVATIAVPQQGSKLPSEETRANLDQMTGHYKRSKFLAELEAVKAAESG
                     VPVIIVNPTAPVGPADWKPTPTGRIIVDFLNGKMPAYVDTGLNLAPVEDVAAGHLLAA
                     HKGRIGERYILGARNMTLKQILEALAQTTGRPAPRVRLPHAVALAAGYANELLSRLSG
                     REPGIPVEGVKMSRHKMFVESGKAERELGYEPGSVEAALARAVCWYRDHGYIGRSPRV
                     KPLSHAQAA"
     gene            complement(2461..4491)
                     /gene="shc"
                     /locus_tag="VN788_10700"
     CDS             complement(2461..4491)
                     /gene="shc"
                     /locus_tag="VN788_10700"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR01507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HXU21040.1"
                     /translation="MTPEPRLQIQGPVAGTAQASSPALPSTAESLIERAIRRSVNWFL
                     SAQNQDGYWWGELEADTTLESDYILYLHILGELQSRRTEKLARYIRDRQLPDGGWNIF
                     YGGPAELNATVKAWVALRLAGDSASSAHLERARAKIHELGGLEATNSYVRFYLAMVGA
                     IDWSFVPAIPPELMLLPRWFPVNLYEMSSWTRGIVIPLAIVYAHKPEWRLPTGITVES
                     LFRNPGRKPPSLAWDKRMISWKNIFLALDRGVKWYDGARWKPFRKLSLALARKWMLER
                     LERSEGLGTIYPAMMNSIFALLAERSGARDSLVAREIGFLARYEIEERDTLRVQPCIS
                     PLWDTAIVMVSLEEAGLDASHPALLSAARWLVDNQILGPGDWQVKNPKAEPGGWAFEF
                     RNDFYPDVDDTAFVLMALSRVAESNSDRLRGAIRRGLAWLASMQNADGGWGAFDHENN
                     LQFLNNIPFADHNAMLDPSTADVTARAVECLGHMGWSASHPVIQRAVAFLKRDQDSDG
                     SWFGRWGVNYVYGTSGVLRALESIGLASLPEAGQAVDWLRSVQNPDGGFGESVLSYYD
                     PKLKAKGRSTASQTAWGLIGLLAVAGPDDPAATRAAAWLISRQNENGTWDEDEFTGTG
                     FPQVFYLKYHLYRNSFPLYALARYDNLRKGRTRSIVTQVAASEIEHPSGYWG"
     gene            complement(4703..5713)
                     /gene="lpxD"
                     /locus_tag="VN788_10705"
     CDS             complement(4703..5713)
                     /gene="lpxD"
                     /locus_tag="VN788_10705"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXU21041.1"
                     /translation="MDRTADELAEYLGAKLEGSGRTLISGVASPERAGATDLIYVDSA
                     QQARRASSSASKCAIAPAGTRFSGKTIIESPQPKFAFAKAAAWLAEVPEPQATIHATA
                     IISPAAKIAEDVSIGPYVVVEQGAEIGASTRIEAFCFVGCDSRIGENCRLHPRVTLYS
                     KVHLGDRVEVHSGSVIGADGFGYVFGEGRHLKFPQIGSVEIADDVEIGANTTVDRGSL
                     DDTCIARGVKIDNLVQIAHNVRIGEHSVIAAQTGVSGSSTLGRGVVAGGQVGVGDHCK
                     IQDGAILGAQAGIPTAKIIRAGQTVWGTPARPLKTFKEQYAWTARLPELADRVRRLEE
                     GH"
     gene            complement(5735..6649)
                     /locus_tag="VN788_10710"
     CDS             complement(5735..6649)
                     /locus_tag="VN788_10710"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXU21042.1"
                     /translation="MLAMREWLEYAAAWMGLKSMGLLPRPVARVVGTVFAAAAYRVRT
                     PLRRAAMFNLRLAFPEWDDAARKRAIRGMVRQIGWMAGEFSQFPKYTRAKIEKLVAVE
                     GFEHFECACRRGKGVLLLTGHMSAWELAPFAHALYGHPMHFLIRPIANGRVDALINFH
                     RCGSGNQPIEKNRSARSILKVLNQGGTVGVLADHNTAIEEGVFVNFFGVPASTTSGIA
                     RLALRTGAAVVPGFLSWSEGQQKYVLQFLPEVQLVRTENEEADIVENTARFSTVIENY
                     VRAHPDQWLWIHKRWKTRPPGEQPLYPF"
BASE COUNT         1425 a         2071 c         1970 g         1406 t
ORIGIN      
        1 aggcaggtat tcaccggttc gacgaaagtg gcttcctcaa aggtcacatc gcgcggaagc
       61 gctatcattc cgccctcgac aatccaaggc atagctcgaa catattcggc aaagccgccc
      121 ccattgggat caaaacccgc agtcaggccg actttcttgt atcccgcgca ttgcgaaaag
      181 agtttttttt tgcaatagaa gcatgcgccg caagggatgt ggtgaaagct gaccacgcga
      241 tcgccgggct tgaatttgct gacgccgcgc ccgacttcaa ccaccgtgcc ggctaactcg
      301 tgtcctagaa tctgaggagg cgggatgaag ttgtggtgaa ttttcttgat gtccgtgccg
      361 cagatgccgc aagccgccac gcggaagagc acttcgcctt cacgaatttc gggaatagga
      421 acgtgttcga ccaccactcg gccttggccc cggtagaccc cggcgcgcat ccggcccttg
      481 cgggtccttg gtggctcggg cgaggctgct tcagcggctg tctcggtcat ctgcggcctg
      541 tcataacttt cgattcgagc gactgtgttc gcttcacgag cgtccccaca tagcgatcaa
      601 gaaatcgcgt caaagactcc gcagcgagtt tgctctgttg cccgaacttc accaagccgg
      661 gaatcgactg cggatggcga gctacttggc ggatgacccg tgaaacgctt acctgccctt
      721 catcggtaaa gatgccattc atgccgagag gcaagtcctc gccggcgacg tcactaatcg
      781 cgcggatggc aaccgcgggg accccgcaag ctgccgccgt ccgcaaaact tcgaaactct
      841 ccatctcgac ggcgtcggcc gattcgccga ggtagcgctt ctcctcagag gtggagacta
      901 cccgcgccgc agtgtggaac ctgctcacta cttttgctcc ttggtccgcg gcgaatgaaa
      961 taaggggttc gctgcttggc aacaattgcg cgcctcgatc ttcgcgcggt ttttcggaaa
     1021 aaacgctgcg agcggcgaga acctgaccga tactatattc agactgaagg gcccccgcca
     1081 atccagacga gatgcacgcg ctcaaggaat cagattccgc gccgatggct ttggatgctt
     1141 gccttgcggc ttgcctcggg ccggcacccg tcagtaccac agcgagttgc gcgccgtgga
     1201 tatcggcaac gtaggcgtcc gcggcaccgt agttcccggc gcaaaattcg cgcagcgctc
     1261 gccagggagc gaactccgct tcgagagcaa atgtaactag aattttcatc agttcaacac
     1321 gctggcgcta cagacggcac gtccccgtca ggcggcttgc gcatgggata acggcttgac
     1381 gcgcggactg cggccaatgt aaccatggtc cctataccag cagaccgccc gcgccagggc
     1441 ggcctccact gagccgggct cgtagcccaa ttccctttcg gctttgccgc tttctacgaa
     1501 catcttgtgg cgggacattt ttacgccttc cactggaatg ccaggctcac ggccgcttaa
     1561 gcgcgaaaga agttcgttgg cgtagcccgc agcaagtgcg acagcgtggg gtaaacgcac
     1621 gcgaggagcc gggcgccccg tagtctgcgc cagcgcctcc agaatttgct tgagcgtcat
     1681 gttgcgcgcg ccaaggatgt agcgctcgcc gattcgtcct ttgtgggcag cgagcaagtg
     1741 accggcggcg acatcttcaa ctggcgcaag atttaggccg gtatcaacgt acgccggcat
     1801 ttttccatta agaaaatcca cgatgatgcg acccgtggga gtaggcttcc aatcggcagg
     1861 accaaccggc gcggtagggt tcacaatgat caccggaacg ccagactcgg ctgctttcac
     1921 agcttctagc tcagcaagaa acttcgaccg cttgtagtgg ccggtcattt gatcgaggtt
     1981 tgcacgcgtt tcttcgctcg gaagtttcga gccctgttgc ggaacggcaa ttgtcgccac
     2041 cgtgctggtg tagacgaagc gttcgacgcc cgcgtttgcc gcggcttcca gcaggcggcg
     2101 cgtgccctga acgttagtct cgtagatctc gtccggattt cgtgcccaca gcctgtaatc
     2161 cgcggcaacg tgaaaaatgt gttggacgcc cgctacggcg cgcttaagcg aggccgcatc
     2221 gttcaggtct ccttcgcaac gctcggcagc gagcccatcg agtgcctgaa gattacttgt
     2281 ccgccgaacg aggactcgaa ccgagtcccc cgcagtgacg agttggcgag ccacatgact
     2341 gcccaaaaaa ccagtggcac ccgtgacgag agtaatcatg atttatatag agccttcgca
     2401 tgccgcaggg gccttcggag tcccaaaaca gaccttctcg cttgtttcgc gcggatctga
     2461 ctacccccag tatccacttg gatgctcgat ttccgaggca gccacttgtg tgacgataga
     2521 cctggtgcgg cccttacgca gattatcgta acgcgccaaa gcgtacagag ggaaagaatt
     2581 tcggtaaaga tgatacttga gatagaagac ttgggggaac cccgttccgg tgaattcgtc
     2641 ctcgtcccag gttccgtttt cattctgacg cgaaatgagc catgcggcag cgcgcgtggc
     2701 cgcggggtcg tctggacccg ccaccgcaag caggccgatg agtccccatg ccgtctgcga
     2761 agcggtactt ctgcctttcg ctttcagctt gggatcgtaa taggaaagca cggattcgcc
     2821 aaagccaccg tcgggatttt ggactgaacg gagccaatcc acggcttggc cggcttcggg
     2881 tagagacgcg agtccgatgc tttccagggc gcgcaagaca ccgctcgtgc cataaacata
     2941 attcactccc cagcggccga accaggagcc gtcggaatcc tgatctcttt tcaaaaatgc
     3001 cactgcgcgc tggattactg gatgcgacgc agaccaaccc atgtggccca agcactccac
     3061 cgcacgggcc gtaacgtccg cagtcgaagg gtcaagcatg gcattgtgat cggcgaaagg
     3121 aatgttgttg aggaattgaa gattattctc gtggtcgaaa gcgccccagc ccccatccgc
     3181 attctgcatg ctggcgagcc aggccagtcc tcggcgaatc gcgccgcgta gccgatcaga
     3241 attggattcc gcgacacggc ttagcgccat caggacgaac gccgtgtcat ccacatcagg
     3301 gtaaaaatcg ttgcgaaatt cgaaggccca ccctcccggc tctgccttcg ggttcttcac
     3361 ttgccagtcg cccgggccga gaatctggtt gtcaaccagc cagcgcgcgg cggagagaag
     3421 agcgggatgc gaagcatcga gccccgcctc ttcgagagaa accatcacga tcgctgtgtc
     3481 ccacagagga gaaatgcacg gctggacgcg cagggtatct ctttcctcga tttcatagcg
     3541 cgccagaaaa ccaatttctc tcgccacaag tgaatctctt gcgccgcttc tctcggcgag
     3601 tagcgcgaaa atcgagttca tcattgcggg ataaatcgtg cccaggccct cgctgcgctc
     3661 aaggcgttcg agcatccatt tgcgcgcgag tgcaagggac aatttgcgga atggcttcca
     3721 ccgcgcgccg tcataccatt tgacgcctcg gtcgagcgcc aaaaaaatat tcttccagga
     3781 aatcatgcgt ttgtcccagg cgagggaggg cggctttctc ccgggattac ggaaaagcga
     3841 ctcgacggta atgccggtcg ggagacgcca ttccggcttg tgcgcgtaca caatggccag
     3901 gggaatcacg atcccgcgcg tccaggacga catttcatac aaattgactg gaaaccacct
     3961 aggaagcagc atcagctctg gcgggatcgc ggggacgaaa ctccagtcga tggcgcctac
     4021 catcgccaga tagaaccgga cgtaggagtt cgtggcttcc aggccgccca attcgtggat
     4081 tttcgcgcgg gctcgttcga gatgcgccga agacgctgag tcacctgcca gtcgcaacgc
     4141 cacccaggct tttaccgtgg catttagctc agcgggtcca ccgtaaaaaa tattccagcc
     4201 tccatcaggc aattggcggt cacgaatata tctagcgagt ttttcagttc ttcgggactg
     4261 caattcgcct aggatgtgga gatagaggat gtaatcggat tccagcgtag tatcggcctc
     4321 gagttcgccc caccaatagc cgtcctggtt ctgagccgag aggaaccaat tgacgcttcg
     4381 gcgaatcgcc cgctcaatga gagattcggc ggtcgaaggc agcgcaggag aggaggcctg
     4441 cgcggtaccc gcaacgggcc cctggatctg cagtcgcggc tcaggcgtca caactccccc
     4501 agacacttct agtcgcttat ttaaaggtct cagcggtaac agtcttagta tctgttcgat
     4561 gcggcctagc gtcaactcaa aacgcctccc gaggcatcac gaggcaatca tcgggatgtt
     4621 cccgggggcc ggaatactgc ttggcggccg tcctatcgat cttttggttc ctatcgccag
     4681 tgttcgccct cgcgtacggt gactaatgcc cctcttcgag gcgtcgaacg cggtcggcaa
     4741 gttccggaag acgggctgtc caggcgtatt gctccttgaa ggttttcaac ggacgcgcgg
     4801 gcgtacccca gacggtttgg cccgcgcgta taatcttggc ggttggaatc ccggcctgcg
     4861 cgccgaggat cgcgccgtcc tgaattttgc aatggtcccc gacgccaact tggccgccgg
     4921 cgaccacccc ccggcccagg gtgctgctcc cggagacgcc ggtctgagcg gcgatcacag
     4981 aatgctcgcc gatccgaacg ttgtgtgcga tttgcaccag gttgtcgatt ttaacgccgc
     5041 gtgcaatgca agtatcgtcc agcgagccgc ggtcaactgt agtattggcg ccaatttcta
     5101 cgtcgtcggc aatctcgacc gagccgatct gcgggaattt gagatgccgg ccctcaccga
     5161 aaacgtagcc gaagccatcc gcgccgatca cagatcccga atgaacttca acgcgatccc
     5221 ccaaatgaac tttggaatag agcgttacgc gcggatgaag gcggcagttt tcgccgattc
     5281 gcgaatcgca gcctacaaaa cagaacgcct cgatcctcgt cgaagcgcca atttccgcgc
     5341 cttgctcaac cacaacgtat gggccgatgg aaacatcctc tgcaattttc gccgcgggag
     5401 aaataatcgc cgtggcatga atcgtagctt gcggctcggg cacctcggcc agccaagccg
     5461 ccgccttcgc gaacgcaaat ttcggctggg gcgactcgat gatcgttttg ccagagaaac
     5521 gtgtcccagc tggcgctatc gcgcacttcg aggccgaaga ggatgcacgg cgggcctgct
     5581 gcgcggaatc gacgtaaatc aggtctgtag cgccggcacg ctcgggactc gcaacgccgg
     5641 aaatgagcgt gcgtccactg ccttcgagct tggctccgag gtattccgcg agttcatctg
     5701 ctgtgcgatc catttttgac ctctctgaat ggcgttaaaa cggatagagc ggttgctccc
     5761 cgggcgggcg cgtcttccac cttttgtgga tccagagcca ctgatccgga tgcgcgcgga
     5821 catagttctc gatgacggtt gagaaacgag cggtattctc gacaatatcg gcttcctcgt
     5881 tttctgtgcg gacaagttgc acctccggca gaaattgaag aacatatttt tgctgcccct
     5941 cgctccatga aagaaagccg ggaacaaccg ctgcgccggt gcgcagcgcc aggcgggcaa
     6001 tacccgaggt cgtcgaggcg ggaacgccga agaaattcac gaatacacct tcctcgatcg
     6061 cggtgttgtg gtcggcgagc actcctactg tgcccccttg attcagcacc ttcagaatcg
     6121 agcgtgccga ccggtttttc tcaattggct gattgccaga accacaccga tggaaattta
     6181 taagcgcgtc cacgcggccg ttggcgatcg gccggataag aaagtgcatc ggatgcccgt
     6241 aaagcgcgtg cgcgaagggc gcgagttccc aggcgctcat gtggccggtc agcaataaga
     6301 cgcctttgcc gcggcggcac gcgcattcaa aatgctcgaa tccttccacg gcgacaagtt
     6361 tttcgatctt cgcgcgcgtg tattttggaa actgtgagaa ctctccggcc atccacccga
     6421 tttgccggac cattccgcga atcgcgcgct tgcgagcggc gtcgtcccac tcgggaaacg
     6481 ctaggcggag gttgaacatc gccgcgcgac gaagcggcgt gcgcactcga taggcggcgg
     6541 cagcgaagac ggtgccgacg accctcgcca cgggcctcgg caaaagtccc atgcttttca
     6601 ggcccatcca ggcggcggcg tattccaacc attctcgcat cgcgagcatt atgcgtgagg
     6661 cctccgtcgc gcgccagtct cggaagtctg ggtttctgaa cccgcaggcg tcgacgcaag
     6721 cgcagttgta cggcttcaca agttcaattt ccattcgccg atgttagact catcagcact
     6781 ttggctgaaa tccgacaagc aaaaagccgc cgagatcccg ctgtgtggct gatcgcgagt
     6841 ctcgtgccgc tgatctgcct cttcggcaac tt
//
LOCUS       DATNDT010000334         4467 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_578359, whole genome shotgun sequence.
ACCESSION   DATNDT010000334 DATNDT010000000
VERSION     DATNDT010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4467)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4467)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4467
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_578359"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1728)
                     /locus_tag="VN788_10715"
     CDS             complement(52..1728)
                     /locus_tag="VN788_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21043.1"
                     /translation="MGALRTLLLHVPSSLLLAPLIFGLAWLWPGLGNSWFSGIEAWFQ
                     SLARRRILTAILLALLPLVLRLTVLPSEGVPIPRVEDEFEHLLVADTLLHGRLANPPH
                     SNPDFFEAVYVLQRPAYSSIYPIGIGISLAIGRLLFGHPWGGVLLFVSLMCAGVYWML
                     LNWVSPGWALIGGVIAIMSFGPTSNWTNSYWCGGAAAFAGCLIFGALPRLIDNSQAFY
                     GLVLGLGLSYAFLIRPFETILAAPCLVIFGFLYRHGISWVHFRPKLMFVALGLVPGVL
                     ITALHNHAVTGKYSELPYALSRIEYGVPQTLSFQRAAVPEHALTPEQTEAYEFQKKDH
                     DSIATLSGWAGSLPGHFSVVRAYLGIALTPLLLVLPFCRFDRQLGWILSSIGLVIVGG
                     SFYAYSQSHYVAPIIGIFFLLGIVTMKRLNEFRWGRTAVRLFLTLAICHFITLYALFA
                     AGNSIPAITGKHKNLTSNADVVTSDPRSRIIDRLRRVGGDHLVFVKYVGHNPRHIQWI
                     HNRADVDSSRIIWARDLGTQADMHLIQHYPSRAAWRVDTGLGEPALTRLR"
     gene            complement(1735..3147)
                     /locus_tag="VN788_10720"
     CDS             complement(1735..3147)
                     /locus_tag="VN788_10720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21044.1"
                     /translation="MKPNLSILIPVYNEEEFISAVLTRALTAPLLDGMTRELVLVDDG
                     STDGSLEICEQFAAQHPDEVRLIRHPGNRGKGAAIRTAVDNALGEFSIIQDADLEYNP
                     NEYPTVLKPLIEGKADAVFGSRFMAVGERRVLYYRHAIANRMLTTLCNLVSDINLTDM
                     ETCYKAFRTSLAQSIPLRSERFGIEPELTIKLAKRQVRIYEVPISYNGRTYEEGKKIG
                     LKDAFEALYVILRTGLSSDIYKDSGPETLEALSVAPKFNRWMADTIRPYIGEHVMEIG
                     AGIGNLTRVLVPRKKRYVLTDIDHEHLARLSTRFKHRPNVEIGFSDLEQSEHFAPYRD
                     QMDSVVCLNVLEHVGDDMQGLQNIRSVLKPGGRAIILVPHDQRIFGTLDTALGHYRRY
                     SHDELRKKMETMGFEVEKIIDFNRISRPAWYVSGRILKRDVLDVRQMKIFDKLVWLWK
                     PLDKVLPWPPTSIIAVGRKS"
     gene            complement(3422..>4467)
                     /locus_tag="VN788_10725"
     CDS             complement(3422..>4467)
                     /locus_tag="VN788_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HXU21045.1"
                     /translation="WRSAEANRSWNRAWNAVDIGPKRDLLLDLMEAGRSRRLRMGIYY
                     SLYEWYNPIYLSDRKRYVAEHLFPQFKDVVTHAKPSIIFSDGEWEMTSAEWRSPELLA
                     WLFNESPVRDEVVIDDRWGKDTRHKHGGYYTTEYTSGMQQSGHPWEESRGMGFSYGYN
                     RMETLKDYHTDRELLMMLIDIVSRGGNLLLDIGPTADGRIPVVMEERLTRMGDWLRPN
                     GEAIYGTRAWRVTRQWSQGTVPKLEDKEFRAAYDITKLVDAPPAGHARVDAFFTATDS
                     AVYAILPRWPAHEIVLDNIHVSPGAQITVLQTGEAVRWSAAGNGLRITTPDTIGTTLP
                     FEEAYVLKIPGGR"
BASE COUNT         1049 a         1252 c         1194 g          972 t
ORIGIN      
        1 gcaggatcga tggtacgtga gttagtagtt tcagaattgc ctgtgtcatt gctaccgcag
       61 acgcgtcaag gctggttctc ctaaaccagt atccactcgc cacgccgccc ggcttggata
      121 gtgttggatt aaatgcatgt ctgcttgggt tccgagatct cttgcccaaa ttatgcggga
      181 gctatccacg tcggcacgat tatgaatcca ctggatatgc ctaggattat gaccgacgta
      241 cttcacaaaa acgagatggt ctcctccgac tcgccgcaat cgatcgatta ttcgcgatcg
      301 cgggtcggat gtaacgacat cggcattaga tgtaagattc ttatgtttgc ctgtgatagc
      361 aggtattgag ttcccagccg cgaagagtgc gtagagagta atgaaatgac agatggccag
      421 cgtcagaaat agccggactg ccgttcttcc ccagcgaaat tcattcagtc tcttcatcgt
      481 tactatgccc agtaagaaga aaatgccgat gatcggcgca acatagtggc tttggctgta
      541 ggcataaaaa ctgccaccca caattactaa gccgattgaa ctaagaatcc agccgagttg
      601 acgatcgaat cgacagaacg gcagaactag cagcagggga gtaagtgcga ttcctaagta
      661 agcccgcaca acagaaaaat ggcccggcaa tgatccggcc catcccgaaa gcgtcgcgat
      721 cgagtcgtga tcctttttct gaaattcata cgcctccgtc tgctctggag taagagcgtg
      781 ttccggtaca gcggcgcgct gaaaactgag cgtctgcggg acgccatatt ctatgcggct
      841 caatgcgtat gggagttcgg agtacttccc ggtaactgcg tgattgtgaa gcgccgtaat
      901 caaaacccct ggaactaaac caagagcgac aaacatcaac ttaggccgaa aatgaaccca
      961 acttatgccg tgacgataaa ggaaaccgaa tatgacaagg catggcgcgg cgaggatcgt
     1021 ttcgaaaggc cgaatcagaa atgcgtagga aagccctaat ccaagtacaa gtccataaaa
     1081 cgcttgcgag ttgtcgatta atctaggaag cgcgccaaaa atgagacacc cggcaaatgc
     1141 agcggcgccg ccgcaccagt agctattcgt ccaattcgaa gttgggccga aactcatgat
     1201 cgcgatcacg ccgccgatca gcgcccatcc ggggctaacc caattcagca gcatccagta
     1261 gacgccggcg cacatcaggg agacaaacag cagcactcct ccccacggat gcccgaacag
     1321 aagcctccca atcgcaagac tgataccgat tccgatcgga tagattgagc tgtaagccgg
     1381 tcgttgcaaa acgtagactg cttcaaagaa atccgggttg gagtgaggtg ggttggcgag
     1441 cctgccgtgc agaagagtat cggcgacgag tagatgttca aactcgtctt caacgcgagg
     1501 aatcggaacg ccctcgctgg ggagaacagt caacctgagg acaagcggca gaagcgcaag
     1561 cagaattgcc gttaaaattc gccgccgggc aagactctga aaccatgcct caatcccaga
     1621 gaaccatgaa ttgcccagtc ccggccataa ccaggctaat ccaaagatga gcggcgccag
     1681 tagcagtgaa gacggtacat gcagcagcag agtccggaga gcgcccatac gcgcttaact
     1741 cttccggccc accgcgatga tggacgtcgg tggccaggga agcaccttat caagcggctt
     1801 ccaaagccag accagcttat cgaatatttt catctgccgc acatcgagga cgtcgcgttt
     1861 caaaatgcgg ccgctcacat accacgccgg gcgggaaatc cggttgaagt cgatgatctt
     1921 ctccacttcg aagcccatcg tttccatttt cttccgcaac tcatcgtgcg agtaccggcg
     1981 ataatgtccc agcgccgtat cgagcgtgcc gaagatgcgc tggtcatgcg gtaccagtat
     2041 gatcgcgcgg ccgcctggct tgagcacgga acgaatgttc tgcaagcctt gcatatcgtc
     2101 gccaacatgc tccaggacat tgagacacac caccgaatcc atctgatccc gatacggcgc
     2161 aaaatgttca gactgctcca gatccgagaa gccgatttcg acgttgggcc tgtgcttaaa
     2221 tcgggtggac agacgcgcca gatgctcgtg atcgatgtcc gttagcacgt atcgcttttt
     2281 ccgcggaacc agcacacgcg tcaggttccc gatacccgcg cctatctcca tcacgtgctc
     2341 cccaatatat ggccggatcg tgtcggccat ccagcgattg aactttggcg ccacggagag
     2401 cgcctcgagg gtctccggtc ccgaatcctt gtagatatcg ctactcaggc cggtgcggag
     2461 aattacatac agcgcctcga aggcatcttt caggccgatc tttttgcctt cctcgtaagt
     2521 gcggccgttg tagctgatcg gcacctcgta aatccgcacc tggcgtttag ccagcttgat
     2581 cgtgagttcg ggttcgatgc caaagcgctc gcttcgtagc ggaatactct gggccagaga
     2641 cgttcgaaac gccttatagc aggtctccat gtcggtgaga ttgatatccg acaccagatt
     2701 gcacaatgtc gtgagcatcc gattcgcgat tgcgtggcga tagtaaagca ctcgccgttc
     2761 accgaccgcc atgaagcgcg atccgaagac tgcgtcggct ttgccttcga tcaacggttt
     2821 taaaaccgtg ggatattcgt tcggattata ttcaagatcc gcatcctgaa taatggaaaa
     2881 ttcgccgagc gcgttgtcaa ccgcggtccg gatcgccgct cccttgccgc gattcccagg
     2941 atgacgaatc aagcgaactt cgtccggatg ttgtgccgcg aattgttcgc agatttcaag
     3001 cgatccatct gtcgagccgt cgtccacaag aacgagttcg cgagtcatgc catctaacag
     3061 tggagccgtt agcgcgcggg taagcacggc ggagatgaac tcttcttcgt tgtaaacagg
     3121 aatcaaaata gataagttgg gtttcatcgc ttattgggcg ttcgcctcta aagagaatcc
     3181 cacggacggg catgtcactg acgttagatc catcattgcg ccgaacctac atagtacaag
     3241 accggatgaa cagccaatta gagtaatgag cgatgaaatg ttttctggtt atcaaacggc
     3301 tgctggtaac ggccggaaac tgtcgatgtt cttgaatccc ggcatgatcg attggtttaa
     3361 gcagaccgca attgcgccca tttgggctcg ccggtgaacg caccgtttct tcctggcgga
     3421 ctcagcggcc gccgggaatt ttaagcacgt aggcctcctc gaacggaagc gttgtcccta
     3481 tcgtatcggg cgttgtgatc ctcagcccat taccagccgc gctccagcgc actgcttcgc
     3541 cagtttggag cacagtgatt tgagcgccag gagaaacgtg gatattatcc aaaacgattt
     3601 catgcgcggg ccatcgcgga agaatcgcgt aaaccgcgct gtctgttgcc gtgaaaaacg
     3661 catccacccg ggcgtggccg gcaggcggcg cgtccaccaa tttcgtgatg tcataggcgg
     3721 cgcgaaactc tttgtcttcc agcttcggaa cagtcccctg gctccattgc cgcgtgactc
     3781 gccacgccct ggttccgtag attgcttcgc cgttcgggcg cagccaatcg cccatgcgcg
     3841 tcagccgctc ctccatgact accgggatgc gtccatcggc tgtcggtcca atgtccaaca
     3901 gcaggtttcc gccgcggctg acaatatcga tcagcatcat cagcagctcg cggtccgtgt
     3961 ggtagtcctt cagagtctcc atgcggttgt agccgtatga aaaacccatg cctcggcttt
     4021 cttcccaggg atgcccggat tgctgcattc cggatgtgta ttcggtggtg tagtatcccc
     4081 cgtgtttgtg acgcgtgtcc ttgccccaac ggtcgtcgat cacgacttca tcgcgcaccg
     4141 gagattcgtt gaaaagccac gccagcagct ccggcgatcg ccactccgcg ctcgtcatct
     4201 cccattcgcc atcggagaag atgatggatg gtttggcatg tgttacgaca tccttgaact
     4261 gcggaaacag gtgttcggcg acgtagcgct tgcggtccga aagatagatc gggttgtacc
     4321 attcgtaaag cgaataatag atgcccatgc gcaggcgcct gctgcgtccc gcttccatca
     4381 ggtcgagcag aagatcgcgc ttcggaccga tatcgacggc gttccacgcg cggttccagg
     4441 accggttcgc ttccgcgctt cgccaca
//
LOCUS       DATNDT010000335         2611 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2829263, whole genome shotgun sequence.
ACCESSION   DATNDT010000335 DATNDT010000000
VERSION     DATNDT010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2829263"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            43..951
                     /locus_tag="VN788_10730"
     CDS             43..951
                     /locus_tag="VN788_10730"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXU21046.1"
                     /translation="MKKLNCVLSIPGDAIYLREQVAIAKTTADRLGVELQVVDAEMDP
                     VNQGQQLLKFVQAPAAERPQGILLEPVSAAGLPRVAEAAVGAGIGWLVSNAQVDYLTT
                     LRRNAKAPVFQVSQDHVEVGRIQGRQMGAILPEGGTVLYLRGPSMSSIASRRFEGLDG
                     AKPKNVLVKSVKVQGSTAESAYTAVASWLNLSTVKPEGTQVIASQNADFIFGARKAFE
                     TTTDAERKKWLALPCAGVGIQKQIKPLVDQGVFRAAVLTSLTMDIALEMLVRAMKEGS
                     QPPEKTFVEAHSYPSLEELAKKRYRA"
     gene            complement(982..1830)
                     /locus_tag="VN788_10735"
     CDS             complement(982..1830)
                     /locus_tag="VN788_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018784 - (S)-2-haloacid dehalogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase type II"
                     /protein_id="HXU21047.1"
                     /translation="MQRREFLAVPVAVGGLMALSSAEAANSDPVPVRSTSLGVKALIF
                     DVFGTVVDWRGSIIREGAKWDKEKGLNIDWGKFADQWRAGYAPSMDKVRKGEMPWTKL
                     DVLHRMILEDLLKQYSIVGLAEEEKDHWNRVWHRLTPWPDAVEGLTRLKKKFVIATLS
                     NGNVSLLVDMAKHAGLPWDTVFSAELFHHYKPDPQAYLGAVELLSCKPAEVMMVAAHP
                     SDLKAAQSCGLKAGLVPRPLEHGPANSAALAKGAAPPIANAAASANESFDITARNFIE
                     LAAKLA"
     gene            complement(1914..>2611)
                     /locus_tag="VN788_10740"
     CDS             complement(1914..>2611)
                     /locus_tag="VN788_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006297685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21048.1"
                     /translation="HPDRFAGLAAIAPQDPSAAAKELQRAVKSLHLKGAIVNSHTRGE
                     YLDEPKFWEIFEAAESLGVPIYLHPNTPSPRMIGPFLGRGLDGAVFGFAVETSLHILR
                     IVFSGVFDRFPKLRMVVGHLGEGLPYWFFRIDFMHQRLASNNRYPNVPKLHKKPSDYL
                     KENFYVTTSGMAWQPPILYAQSVLGMDRVLYAMDYPYQFVPEEVKVTDDLPISGADKK
                     KFYQTNAEKVFAL"
BASE COUNT          553 a          695 c          826 g          537 t
ORIGIN      
        1 ggaaatccgc gctaccatta aatgcgtacc ttgggaacaa ccgtgaaaaa attgaactgt
       61 gtgctgtcaa ttcccggcga tgccatttat ttgcgcgaac aagtggcgat tgcaaaaacc
      121 acggcggatc gcctgggagt ggagctgcaa gtggtggacg cggagatgga cccagtgaat
      181 cagggccagc aacttctgaa attcgtgcag gcgccggccg cagaacgtcc gcagggcatc
      241 cttctggagc cggtgagcgc cgccggattg cctcgtgtgg ccgaggccgc ggttggcgcg
      301 ggaattggat ggctggtcag caacgcgcag gtcgattatc tgacgacgct ccgcagaaac
      361 gcgaaggctc cggtatttca ggtttcgcag gaccacgtgg aagtgggacg gatccaaggg
      421 cgacagatgg gcgcgatttt gccggaaggc ggcacggtgc tgtaccttcg gggaccgtcg
      481 atgagctcga tcgcgtcgag gcgattcgag gggcttgacg gcgccaaacc caagaatgtg
      541 cttgtgaaga gcgtgaaagt gcaaggctcg accgcggaaa gcgcttacac cgcagtcgct
      601 tcctggctga atctttcgac agtgaagccg gagggaacgc aagtgatcgc gtcgcaaaat
      661 gcagatttta ttttcggggc tagaaaagcg ttcgaaacaa caaccgatgc ggaacgaaag
      721 aaatggctgg cgctgccgtg cgcgggcgtg ggcattcaaa agcagatcaa gccgctggtg
      781 gatcaaggtg tgtttcgggc ggcggtgctg acttccctga ccatggatat cgcgctggaa
      841 atgctggtgc gggcgatgaa ggaaggttcg cagccgccgg aaaagacgtt tgtggaggcg
      901 cattcgtatc cgagcctgga agaactcgcc aagaaaaggt accgggcgta gcggccgctt
      961 tcgcgtgatt ccgaggcgtc gctaagccag cttcgcggca agctcgatga agttgcgggc
     1021 ggtgatatca aaggattcgt tcgccgaagc agcagcgttc gcgattggtg gggctgcacc
     1081 tttcgccaat gccgcgctgt tcgcgggacc gtgctccaaa ggacgcggca cgagacccgc
     1141 cttcagcccg cacgattgcg ccgctttcaa atcagagggg tgggcggcca ccatcattac
     1201 ttcggcgggc ttgcagctga gaagttccac cgcgccaaga taagcctgcg gatccggttt
     1261 gtagtgatgg aaaagctccg ccgagaaaac ggtgtcccac ggaagtcccg cgtgtttggc
     1321 catatcgacc agcaaggaaa cgttgccatt tgaaagcgtg gcgataacaa attttttctt
     1381 gaggcgcgtg aggccttcga ctgcatcggg ccacggggtc aagcggtgcc acactcggtt
     1441 ccagtggtcc ttctcttctt ccgccaggcc gacgatgctg tactgcttga gcaggtcttc
     1501 gaggatcatg cgatgcagca cgtcgagctt ggtccaaggc atctcgccct tgcgaacttt
     1561 gtccattgag ggagcgtaac ccgcgcgcca ctggtcggcg aactttcccc agtcaatatt
     1621 taggcctttc tctttgtccc atttggcgcc ctcgcgaatg atcgagccgc gccaatccac
     1681 cacggtgccg aacacgtcga agatgagcgc tttcacgcca agagaagtgg agcgcactgg
     1741 caccgggtca gaattcgctg cttccgcgct cgacaatgcc atgagacccc cgaccgcaac
     1801 agggaccgcc agaaattcgc gcctttgcat tggactctcc taatttgcgg ttgcggattt
     1861 cgtgcgccgc attctaaccc aaaagcccgg agggttcgct tcgaccgagt gagttacagc
     1921 gcgaacactt tttcggcgtt ggtctgatag aacttctttt tgtccgcgcc gctgatgggg
     1981 agatcatcgg tgactttcac ttcttccggc acgaattggt agggataatc catggcgtag
     2041 aggacgcggt ccatgccgag tacggactgc gcgtacaaaa tcgggggctg ccacgccatt
     2101 ccgctggtgg tgacgtagaa gttttctttc aaataatcgc tgggcttttt gtgcagcttg
     2161 ggaacgtttg ggtaacggtt attggaagcg agccgctggt gcatgaaatc gatccggaaa
     2221 aaccagtaag gaaggccttc gccgaggtgc ccgacgacca tgcgcaattt tggaaagcgg
     2281 tcgaatacgc cgctgaaaac aattcggaga atgtggaggc ttgtttcgac ggcgaatccg
     2341 aaaaccgcgc cgtcgagacc gcgaccgagg aacggtccga tcattcgggg cgagggcgtg
     2401 ttgggatgca gataaatcgg aacgcccagc gattccgcgg cctcgaaaat ttcccagaac
     2461 ttgggctcgt caagatattc gccgcgcgtg tgggaattga cgatcgcgcc tttgaggtgc
     2521 aacgacttta cggcgcgctg taattctttc gctgcggccg acgggtcctg cggcgcgatc
     2581 gcggctagtc ccgcaaagcg gtcggggtgc t
//
LOCUS       DATNDT010000336        21777 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_646738, whole genome shotgun sequence.
ACCESSION   DATNDT010000336 DATNDT010000000
VERSION     DATNDT010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 21777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21777
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646738"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="VN788_10745"
     CDS             complement(<1..302)
                     /locus_tag="VN788_10745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HXU21049.1"
                     /translation="MLRPLLAAAVTLAAALPALAQGTPATLFNHGAGRWYLAGQANYI
                     YQEHGGFPALYSGPNSLINSPEDALSRVLTVYTGYQLRPSIEAIFDVEETGGQGLST"
     gene            657..1016
                     /locus_tag="VN788_10750"
     CDS             657..1016
                     /locus_tag="VN788_10750"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HXU21050.1"
                     /translation="MPCALRRGAERPDRLIVGEIRGEEAYDLLQALNTGHSGSLSTIH
                     ANSARQAITRLRSCILQAGFEIPHRALQSQIADSVQIIVHVERAGGRRRVAEIVEIAG
                     FDAESDRHQFNTLYQCG"
     gene            1031..1855
                     /locus_tag="VN788_10755"
     CDS             1031..1855
                     /locus_tag="VN788_10755"
                     /inference="COORDINATES: protein motif:HMM:NF028103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-primase RepB domain-containing protein"
                     /protein_id="HXU21051.1"
                     /translation="MEPAAPVTSWDFIKQNYRADDRIAIVIRNRHEDYTLQRLLSAQD
                     AASPRIQRFLRYENAHGADIYVSMNILKPAAKSRTKADIAEISHLYLDLDEDGDKRLA
                     RVLKGDALPQPNYVLDTSPGKHQVIWKVEGFTLEQAEAALRAMAREFGGDPAATDAAR
                     VLRLPGLHNKKFKEPVRVQGQRLYEGVYRPEKFATLPAIPEACLTNPGKPHVPLGTRR
                     DGGPLSQSERDWAWVRNQLRAGASPEAVRRELVGRRQDKSDPTNYAERTVWRALSR"
     gene            1911..2255
                     /locus_tag="VN788_10760"
     CDS             1911..2255
                     /locus_tag="VN788_10760"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU21052.1"
                     /translation="MKTLGERIRELRETKDLSVRELARRLGITAPFLSDVELGRRHPK
                     DEVLKAIASHLGTTFEELKKHDSRPPVQELKRMAAANPAMGFALRRVVDEGIKPEALL
                     KFLNTQGGAKKK"
     gene            2252..2953
                     /locus_tag="VN788_10765"
     CDS             2252..2953
                     /locus_tag="VN788_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020494369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21053.1"
                     /translation="MRTFRPKSGPFAEQPFFEPNEIESICIEALQKVALLPSEPAPIR
                     IDRFIEKRFGVQPGYEELPKGILGFTRFGTKGVEEIVVSKSFDDEGTVVAERRLRTTL
                     AHEGGHGLLHAHLFAVDKRPGSLFGDGLAPDEPKLLCRDEGGSAGRPAPSRWWEFQAN
                     QAMGVLLLPKPLVVQALTPMLKTQGSLGLATLPTGQRGAAVRLVSDVFNVNPVVARIR
                     IDGLYPPQGGAQMVL"
     gene            3047..3304
                     /locus_tag="VN788_10770"
     CDS             3047..3304
                     /locus_tag="VN788_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21054.1"
                     /translation="MTEPEANIAQGHKVSVTIDGHEKEIQAREYPVMQLKAALGVDPT
                     RDLDQVIDGQLVPLSDSDKIHIRGGEVFISHVRGGGSSHDL"
     gene            3294..3635
                     /locus_tag="VN788_10775"
     CDS             3294..3635
                     /locus_tag="VN788_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21055.1"
                     /translation="MISEKELVELHPLCDGDLARECPEFDHTYILLPKMGLPAGCRPV
                     RVDALLCLTGRDGYPTRLYFAERVACKAQLNWNAQDVRILERTWFAYSWTGVARGRPI
                     EVLAQHLKALK"
     gene            3632..4138
                     /locus_tag="VN788_10780"
     CDS             3632..4138
                     /locus_tag="VN788_10780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21056.1"
                     /translation="MNIHFKLTTSMHRQILDDLRRTHPFAAERAAFIGCKPSRSRTGI
                     LLLAHTYIPLWDEQYVNDQRFGCVFNSEAMRNAMQFALQSDASMFHVHLHEHVGPAWF
                     SRTDLRESQCFVPDFWNVKPQLPHGALVLSADAAAGLCWYPGQPIPARITQISSIGYP
                     SRLLERLT"
     gene            4135..4935
                     /locus_tag="VN788_10785"
     CDS             4135..4935
                     /locus_tag="VN788_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009548465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HXU21057.1"
                     /translation="MNERFSRQSFLGQQSDAIFQSLRAGIAGLGGGGSHIAQQLAHLG
                     VGHFVLFDPDRFETPNLNRTVGATFADACSATPKTLIAERLIKGINPCAEVRSIASVW
                     QANLDDLRECDAVFGCLDGFSNRAQLEESCRRAMIPLIDIGMDVHKGAPYSISGQVVV
                     SLPGQPCFRCFGFINERDLVDEGRRYGEAGGRPQVVWSNGVLASTAVGLFVQIFTPWC
                     SPRPGCVFVGYDGDAFTLEHDGRQSIVGGRQCSHFAAIDDLGDPFWRP"
     gene            5001..6248
                     /locus_tag="VN788_10790"
     CDS             5001..6248
                     /locus_tag="VN788_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21058.1"
                     /translation="MGTSVGNAERSPARAHISLDSRVATRDTRFMEDDTSFIGEVIRI
                     INGALRLDIDKVRNYTAFLAEKLEKAGNKDAANRLRKVLEEGDHQLRPAGASFARTLP
                     VDSESRFALLERVNLKALSEPPVILSQEQRDVVHEFLSIAKSHAQLDVDGSALSLGLL
                     MYGPPGTGKSRLARLIAQELGLDLYIARLDGLISSFLGSTSKNIRALFDFAARTPCIL
                     FLDEFDAIAKLRGDNQELGELKRVVNSFIQNIDSLGNQSIVIAATNHQELLDSAIWRR
                     FGYRLALDFPNADSRKQMWLQFSPPQEFSPREIELLVDLSEGFSGSDIRDACLRLYRR
                     RITAQKAPDLRDAFQVLRNLAIGEGEGRRFITQFQTKDHHALAAALRGRNPRLYSHAA
                     LADLLGVSKATSYRWSKGVKSNG"
     gene            6565..8595
                     /locus_tag="VN788_10795"
     CDS             6565..8595
                     /locus_tag="VN788_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012806702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HXU21059.1"
                     /translation="MKATPEGLDRLADIIKRNHSDRMVKEISCVEAIEPVTPLFRRGG
                     IEANDVLRRSQRGKKGFITRVRLFNFGGAGDQQRVVSDFETTCRKHGIRASRNGYSPS
                     SFTYAAECGDVGGVEAISRIAGVRSISYMPLIRTVRPRLLNHKPLPILPSRAEGEGDF
                     PVVVVVDSGVSDLMPALESWVVGRDSQVAPEYRNTDHGTFVAGLICWGSKLNPTLSGI
                     DNSPCGIFDLQVIPNPDPAKGDTLALLEQELLISLDSALREHANKYKVWNLSLGTDAI
                     CSLDEFSVLAEELDNLQEKYQVSFVISAGNYATPPLLDFPRTAEQIDAGRITAPADSV
                     LGITVGSVSHVGYKESGPREHHPSPFSRHGAGPNYVIKPDLVHYGGSCSTDLAHIAGI
                     RSICGDNSAENLGTSFAAPLVARTLAHIYHQIMPTPTPVLARALLTHHARDPRTRRRV
                     PDGDENFFGFGLPAPVPYCLECTPYTSTLVFDDVLRPGYFLEWDDFPYPPSLRRNGRY
                     FGEIWMTVAFSPVRGSRWGTEYCETHIDAHFGVYRAQKSRETGAVKTKFTGLVPPEHK
                     NVGILYESYQVEKLRKWAPVRTYYGDLGEKGERGNRWRLKLQLLTRHGIEEEESFKPQ
                     PFSLIVTISDPQKKAGVYDEMAQNLRTRFQAENLTIRAAARVRAQRTPTVHK"
     gene            8592..8804
                     /locus_tag="VN788_10800"
     CDS             8592..8804
                     /locus_tag="VN788_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013697136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HXU21060.1"
                     /translation="MSKTKDQLFVERRQQGDYAVRRGGAERASATAPTQAEAIDIARN
                     LNSGKAPLVERVRDTKFGHRDEWRKA"
     gene            complement(8849..11671)
                     /locus_tag="VN788_10805"
     CDS             complement(8849..11671)
                     /locus_tag="VN788_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009454389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF499 domain-containing protein"
                     /protein_id="HXU21061.1"
                     /translation="MPSLKPWYKVVTPREDLRSGRPLDASEFAVHLDHIRDGRAPEVY
                     QDPAAFFDRTYLTKNLSDLAMQVVRRLSGMKVETSAVFNLSTQFGGGKTHALTLLYHL
                     ARGGAEARSWKGVSGLLQDAGVTAVPSAATGVFVGTEFDSITGRGGADGTPLRKTPWG
                     EIAYQLGGIEGFSLVEEHDRSLTAPGGDVIEKLLPAGRASLILLDEVMNYVGRSRKSG
                     MAAQLYSFLHNLSEVARARDKVVLAVSIPASELEMTAEDQSDFERFKKLLDRLGKAVI
                     MSAESETSEIIRRRLFEWSGIPKDAAATLQEYEDWLQAHKQQLPGWFPTENSRELLKA
                     SYPFHPALLSVFERKWQTLPRFQQTRGVLRILALWVSRAYSDGYSGAHKDPLITLGTA
                     PLDDSLFRAALFEQLGEARLEAAVTTDIAGKSDSHAVRLDEEATVEIKKRRLHRKIAT
                     SILFESNGGQQRAEATIPEIRFAVGEPDLDIGNIEQCLESLTDTGYFLSAERNRYRFS
                     FQPNLNKLLADRRAAIDTTAIEERVRQEIRIVFGVRPGSSSTTTPGIDRIYFPTKSGE
                     IPDRPVLALVVVPSAMHALDPATKKQILGMNGESGSSSRTFKTALIWAAVDDGAPLVE
                     AVRKQLAWEEIEADSDSLKLDEAQQRQLRENAKRAERDMREIVWRGYRHIFLFAEGND
                     LRHIDLGLVHSSSANSLTELILTRLSEEDIVADGVSPNLLVRTWPPALPEWSTKGVRD
                     AFYASPTLPRLLKPDAVKETISRGLDAGTLAYVGKAEDGHYAPFVFKRSLPASDVEIS
                     DDVYLITRERAEAYLAQRAGAGAAGTTAEGGPTLFPSGLESAVGAKSLGAIGANDQGV
                     LPGDQAPAPSASGDVASLHWSGEIPPQKWMNFYTKVLSRFATGPGLNIKIQVEVAPPA
                     GIPKSKVDDTKAALRDLGMPDDVGTE"
     gene            complement(11677..12243)
                     /locus_tag="VN788_10810"
     CDS             complement(11677..12243)
                     /locus_tag="VN788_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005987007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HXU21062.1"
                     /translation="MTDQKRAIWIVDAAYLLKASPGKFDYLKLKVLLAGENGGEFTEA
                     YYFNSTPNPPTDGQDAFHTWLKFAPPKGPKMRVQLYKLKNVSTRCSSCGKTDIREVQK
                     GVDVGIATLILKLAARNQYERLVLSAGDGDFEDAIDYVKSGQQKEIWIAGFQNSVSAD
                     LQSYADRVVFLDDHWQEIAKDHTGAAGD"
     gene            complement(12240..15992)
                     /locus_tag="VN788_10815"
     CDS             complement(12240..15992)
                     /locus_tag="VN788_10815"
                     /inference="COORDINATES: protein motif:HMM:NF020302.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADAR domain-containing protein"
                     /protein_id="HXU21063.1"
                     /translation="MVPTIPEENKLMGRAEQRTYIRADSVSFRKTDEEFGGLSNMAGG
                     FPLEINGERIRTSEALYQACRFPHLPDVQRIIISEKSPMTAKMKSKPFRGQSRSDWVR
                     VRVKIMRWCLRVKLAQNWETFGRLLLGTGDGPIVEDSRKDDFWGAKPAEEDVLIGNNV
                     LGRLLMELREALRGPDRDQLCRVEPPSIPQFLLFNEPIGAVEARRRTNAPPRSSGTGY
                     IAGSKPLTVKSTHVPSASGVIAATSGEKSSDRSIELPVAPRCWPKRLIEVDLPIKRIS
                     AHARREKSIRPGHISTLHIWWARRPLASCRAVICATLWPDPADALCPESFRKIAREEM
                     AKWALDHVKLLGLESQERFLTLRKEPGKILDNTELRRALLDFIADFANWDNSTVPAYL
                     ETSRALTQAAHEGLGGEPGTRPLVVDPFAGGGSIPLEALRVGADAFASDLNPVAVLLN
                     KVVLEYIPKYGERLADEIRKWGEWVKREVEKELTDLYPKDPDGAAPIAYLWARTIHCE
                     GPGCGAQVPLIGRTVIAQRKQGGVGIAFRSSDGSKGVAISLVEADDQSIAKATCRGFV
                     ATCPLPGCGFSTPKKSVQRQLSAQNGGADNSRLVAVLTTGPSGRRYREPGPLDEMAVM
                     ASKAALQKIVNAARSSSSVIPDEQVPQKGAHRAVGSQLPLYGFSTWGSLFTARQQVTI
                     LALQRAVRRAYELLPTALDEHFARAVFLTLAFAVDKQADYSSSLCRWVPSGEFIAGTL
                     AGEKKLPMISDYAESVPLGSGSGSWDNQIDWIARLISREATASSQTGTICQSSAEECP
                     LPSCAASALITDPPYYDSVPYADLSDYFYVWLKRTTHGIERLENLRKDREMTVNHPDS
                     QEEKERYEELLTQAFSKGRDFTAFSGIAVIVFAHKSTSGWEALLEAIINSGWIVTASW
                     PIDTERAARPNALKTASLASSVHIVCRPRENTAAGAGNEVGDWRNVLNELPSRIHEWM
                     PRLAEEGVVGADAIFACLGPALEIFSRYSRVEKASGEPVTLGEYLVQVWAAVANEALA
                     LVFRGADASGFEEDARLTAMWLWALSANRNGTTAEDGTGGDPEAGDDSEDVTPAISTG
                     YALEYDAARKIAQGLGAHLEALTSLVEVKGDTARLLPVAERTRRLFGKDEDGPAASKP
                     RKKKDPQLKLGFVAELERAEGAGSWGVKGAPTLGATVLDRVHQAMILFAAGRSEAIKQ
                     FLIDEGVGRDDRFWRLAQALSALYPAGSDEKRWVDGVLARKKGLGL"
     gene            complement(15959..16948)
                     /locus_tag="VN788_10820"
     CDS             complement(15959..16948)
                     /locus_tag="VN788_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21064.1"
                     /translation="MPSSERKALLAAVASTIADYRLGEIPPLDENHVATWIGQFEQPV
                     QEPMLAELDHVLKKTYISKPKVEAFLTSVLQSRQLVGASPCDFWRSANFLDIQGGGSS
                     QREMREMFSQQLKHVCGLRIDQCGLAYGPYVYLDDVIFTGNRVRSDLRNWINSSAPAN
                     VKVHVIVIAFHRGGNYYAKTGIAAASTAAGKTIDVTFWRHLEIEDRKARNAYADVLRP
                     ASIPTDDLTQAYVQSLTHPPLVRTGTLLGENNFFFSDSGRNLLEQELLKAGLRIRSQC
                     PYLNIYQRPLGNMVLASLGFGATIVTFRNCPNNCPLAFWAGDPWYPLFPRKTN"
     gene            complement(16938..20387)
                     /locus_tag="VN788_10825"
     CDS             complement(16938..20387)
                     /locus_tag="VN788_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020506619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HXU21065.1"
                     /translation="MTPPTFEDLCPGAVVEGPILPEPVEVLAIVSMGTAVKLVGKGLH
                     TGQVHEPILDQVQVAQLALTPKDAPFDGDPLHFKLGIEAARLGLAYEYDPYFSLSVAR
                     VDPLPHQLEAVYDYFLKLPRMRFLLADDPGSGKTIMAGLLLKELKIRGLVKRVLIVAP
                     ANLTFQWQREMRDKFREQFDVMRGEVLRSQYGQNPWQERNQIVTSVSWISRVDDAKES
                     LLRSNWDLVIVDEAHKMSAYSSDKKTLAFQLGEALSQRTDHLLLMTATPHKGDTENFR
                     LFLSLLDRDVYGDVKSLEEAMRRHSAPFYLRRLKEALVTFPDPDSGEVKKLFTNRDVR
                     TTRFELDGDEFDFYDALTRYVEDQSIKAAAAENVQASILSFQMAMLQRRFASCIYAVR
                     RSLERMRDKRCKILADPEKHRREQIEKKLPDDFEELPEDEQAKIIADAEGAVLIIDPV
                     SLRDEIQRLEKLIEQARALEQREIESKLTKLRQVLNEQHIFSDPRMKLLVFTEHKDTL
                     DYLAGDGHDGRPLGKLREWGLSVTQIHGGMQIGDRDTPGTRIYAEREFREDCQVLVAT
                     EAAGEGINLQFCWLMINYDIPWNPVRLEQRMGRIHRYGQENDCLIFNFAAVNTREGRV
                     LEKLFLRLAEIKEELGSDKVFDVIGQMLPSNQLEKMFRDMYAKRTSESNIADRIVRDI
                     DPERFRHITGATLEGLAKKDLNLSALVGKSVEAKERRLVPEVVEDFFKAGGPVASVHP
                     AAVRGKEHVYRIGKVSKSLSAIGERQEPRFGKLGREYQRVVFDKRLLGDDATLEWVTP
                     GHPLFEAVRTEVWDRVANDLRRGTVLWDLQTSEPYRLDVFTAAIKDGRGNTLHKRLFV
                     VRTDPDGTLTARQPTLFLDLIPTPKGTTEPALHNLPDKTAVEVHLIQTTLQPFRDEIG
                     AERTAENRIVREHIEISLGALIDRQQNVLGDLLNRRIASGGAAGLEGNIVQAENHLDE
                     LNARLERRRSELDMERHCTIGDIQHLGQAWVLPHPDRGAPGIAPMVQDAEIEKIAVRV
                     AMEHERENGWVVESVETENRGYDLLSRKPHPTEPGVFAAARFIEVKGRAGVGEVALSA
                     NEYRTAERLKNDYWLYVVFNCGTKPELHAIRDPARLGWQPIVQVAHYHLCAKEILGGE
                     NAE"
     gene            complement(20384..20638)
                     /locus_tag="VN788_10830"
     CDS             complement(20384..20638)
                     /locus_tag="VN788_10830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009379087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU21066.1"
                     /translation="MTWSLMPRYTSKPLGIESPAAQEVLTIREVAAILKLAGKTVYAM
                     ANAHELPAFKVRGLWRIRKVDLQHWMTEQAHAAAHQRQNK"
     gene            complement(21027..21218)
                     /locus_tag="VN788_10835"
     CDS             complement(21027..21218)
                     /locus_tag="VN788_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21067.1"
                     /translation="MRIQRLDAFNVCPWIALCLRRGRQGYPHRLRHIEHKGAAPVVSA
                     EIGGSGWGWKVEVDSAVMI"
     gene            21334..>21777
                     /locus_tag="VN788_10840"
     CDS             21334..>21777
                     /locus_tag="VN788_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21068.1"
                     /translation="MHELAHELLHGGERRKQLSRKVKETEAEAVAYAVGLPVGLDMNT
                     ASAHYISLYDGDRDTLRESLDSITRVSSEILQGGGEGQGGGKQGAQHGVAQGRLRPRT
                     PPYTGGGILSTDWRGNRAARHPWPTPKTRRSASSTASTAFRARWLR"
BASE COUNT         4344 a         6580 c         6357 g         4496 t
ORIGIN      
        1 gtgctcagcc cctgtccgcc ggtctcttcg acatcgaaga tcgcctcgat cgaggggcgg
       61 agctggtatc cggtgtacac ggtgagcacg cgggagaggg cgtcttcggg gctgttgatc
      121 aggctgttgg ggccggagta gagagcggga aaaccgccgt gctcctggta gatgtagttg
      181 gcctggccgg cgagatacca gcgcccggcg ccgtggttga aaagggtcgc gggggtgcct
      241 tgggcgagcg ccggcagggc ggcggcgagg gtcacggcgg cggcaagcag ggggcgcagc
      301 atggcgttgc tcagggcaga ctgcgacccc gaaccccacc gtataccggc gggggggata
      361 cggtcaatac gcgggggtcg cgccgttggc accgtgggcc aagtcggcgc gaaaaggccg
      421 cgaaacgccg ccccgcgcag ttccagcgtg ggcggcaatt ccgcaggggt gaaagcgcca
      481 gtggcgcttt cacgcccccg gcaagcaaca aggcgcggcc ccggccggct ggggctccca
      541 gggggtgcga gcgtctcagc gagcacgccg gccgagcggg ccgcccgcgc agttccagcg
      601 gggcggccgc ataagcgggt cgctcggcgc agccgagcgg ggtcccgcgc cagtcaatgc
      661 cctgcgccct gaggaggggc gcggaacgcc ccgaccggct gatcgtcggc gaaatccggg
      721 gcgaggaagc ctacgacctg cttcaggcgt tgaacaccgg ccattccggg agcctgtcca
      781 cgattcacgc caactcggcg cggcaggcaa tcacccggct ccgcagttgc atcctgcagg
      841 ccggcttcga aatcccccac cgagcgctcc aaagccagat cgccgactcg gtccagatca
      901 tcgtccacgt cgagcgcgcc ggcggccggc gccgggttgc cgaaattgtt gaaatcgccg
      961 gattcgatgc cgaatccgat cggcatcagt tcaacacact ctaccaatgc ggctgacaag
     1021 gaggcatcgc atggaacccg ctgcgcccgt cacctcgtgg gacttcatca agcagaacta
     1081 tcgggctgat gatcgaatcg ccattgtgat ccgcaaccgc cacgaggact acaccctcca
     1141 gcgactgcta tcggcccagg atgcggcctc accgcgcatc cagcgtttcc tccgttacga
     1201 aaacgcccac ggcgccgaca tctacgtctc catgaatatt ctgaagcccg cggccaagag
     1261 ccgcaccaag gcggatatcg ccgagatcag ccacctatac ctcgacctgg atgaggacgg
     1321 tgacaagcgg ctggcgcggg tgctcaaagg cgatgcgttg ccgcaaccaa actacgttct
     1381 cgacacttcg cccggcaagc accaagtcat ctggaaggtc gagggcttca cgctcgaaca
     1441 ggccgaggcc gccttgcgcg ccatggcgcg ggaatttggc ggcgatcccg ccgccaccga
     1501 tgccgcccgg gtgctgcgcc tgcctggctt acacaacaag aaattcaagg aaccggttcg
     1561 cgtgcaggga cagcggctct atgagggcgt ataccggccc gaaaaattcg ccacgctgcc
     1621 tgccatacca gaggcctgcc tcacaaaccc agggaagccc catgtgcctt taggcactcg
     1681 ccgcgacggc ggtccgttga gccagtccga acgggactgg gcttgggttc gcaaccagct
     1741 acgcgctggt gcgtcaccgg aggcagtcag gcgagaactc gtcggacgcc gccaggacaa
     1801 atcagatccg acgaattacg cggaaaggac ggtgtggagg gcgctgtcgc gctaaaagac
     1861 caccgcttga cccgagccca tgtcgagtgt aagctttcat gcggtacaca atgaaaactc
     1921 tcggggaacg tatccgtgaa ctgcgtgaga caaaggatct ctcggttcgc gaacttgcga
     1981 ggcggctcgg tataaccgcg ccttttttgt cagacgtgga gcttgggcga aggcatccga
     2041 aggacgaggt gctgaaggca attgcctccc atcttggaac tacatttgag gaactcaaga
     2101 agcacgattc tcgtcctcca gttcaggagt taaaacggat ggcagcggcc aacccagcga
     2161 tggggtttgc attgcgaagg gtagtcgacg agggcattaa gcccgaggcg ttgctaaagt
     2221 tcctcaacac gcaggggggg gcaaagaaga aatgaggacg tttcgaccta aatccggccc
     2281 cttcgcggag cagccgtttt ttgagcccaa cgaaattgaa tcaatctgca ttgaggcgtt
     2341 gcagaaggtt gctctgctgc catctgaacc cgcccctatc aggattgatc ggttcatcga
     2401 aaagcgattt ggagtgcagc ccggctacga agaactgccg aagggaatcc tcggattcac
     2461 cagattcgga accaagggcg ttgaggagat cgtcgtgtcc aagtcgttcg acgatgaggg
     2521 cacagtggtc gcggagcggc ggctcagaac aaccctcgcg catgagggcg ggcacggatt
     2581 gctgcacgcc cacctttttg cagttgacaa gcggccgggt tcccttttcg gtgacgggct
     2641 ggctcccgac gagcccaagc ttttgtgtcg cgatgaagga ggttccgctg gtcggccggc
     2701 gccatcccgt tggtgggagt tccaggctaa ccaagcgatg ggcgtactcc ttctcccgaa
     2761 accactggtt gtacaggccc tcaccccaat gttgaagaca caaggcagcc ttggactggc
     2821 cacgctccct actggccagc ggggcgcggc ggtccgcttg gtttctgatg tattcaacgt
     2881 caatccagtg gtcgcgcgga ttaggataga cggactgtac ccgccccagg gcggcgcaca
     2941 gatggtgctg taaaaaaatt tgcgtcgcgt gtaagcttgt ggtagcttac acctgggtcg
     3001 tcggctgcgt ggccagccga atcacaagcg acgcaaggag atgtcaatga ccgaaccaga
     3061 agcaaacatt gcgcagggac acaaggtgtc cgtgacgatc gacggccatg aaaaagagat
     3121 tcaggctagg gagtacccgg tgatgcagct caaggcagcg ctcggagtag atcccacgcg
     3181 cgatctcgac caggtcatcg acggtcaatt ggtaccgttg agcgacagcg acaaaattca
     3241 cattcgaggt ggggaggtct ttatcagcca tgttcgcgga ggaggctcat cccatgatct
     3301 ctgaaaaaga actggtcgaa cttcaccccc tctgcgacgg cgatctcgct cgcgagtgtc
     3361 ccgagttcga tcatacatat atccttttgc ccaagatggg cctgccggcc gggtgtagac
     3421 ctgtccgggt cgacgcgctc ctgtgcctga ctggaagaga tggctatccc acgcgtttgt
     3481 attttgccga acgggtggcg tgcaaggcac agttgaattg gaacgcacag gatgtccgga
     3541 ttctcgaacg gacctggttc gcgtactcgt ggacaggcgt agctcgcggt cgtccgatcg
     3601 aggtgctggc tcaacatctc aaggcattga aatgaacatt cacttcaagc taacaaccag
     3661 catgcatcgc cagatcctcg acgaccttcg gcggactcat cctttcgccg cagaacgggc
     3721 tgcgttcatc gggtgcaagc cgagccgaag caggaccggc attttgctgc tcgcgcacac
     3781 ctacattccg ctttgggatg agcagtatgt taatgatcaa cgctttgggt gcgtgtttaa
     3841 ttccgaggca atgcggaatg cgatgcagtt cgccttgcaa agcgacgcca gcatgttcca
     3901 cgttcatctg cacgaacacg ttggacccgc atggtttagc cggactgacc tgcgcgagtc
     3961 tcaatgcttc gtgccggact tctggaatgt aaaaccccag ctaccgcacg gcgcgctcgt
     4021 tctaagcgca gatgccgcag cgggtctgtg ctggtatcct ggccagccca ttccggcacg
     4081 gattacacag atcagttcga tagggtatcc gagcaggctg cttgagaggc tgacatgaat
     4141 gagcgcttca gtcgccagag tttcctggga caacagagtg acgcgatctt ccaatcgttg
     4201 cgtgcgggga ttgctgggct tgggggaggg ggatcgcaca tcgcccagca attggctcac
     4261 ctcggggttg gacactttgt cctatttgac ccagaccgct tcgagacgcc gaacctcaac
     4321 cgaactgtcg gcgcgacgtt tgctgacgct tgtagcgcta ccccaaaaac gttaatcgca
     4381 gaacggctga taaaaggaat caacccgtgc gcggaggtgc ggtcaatcgc atccgtgtgg
     4441 caagcaaacc tcgacgatct gcgggagtgt gatgcggtgt tcggttgcct ggatggattc
     4501 agcaatcgcg cacagcttga ggagagctgc cggcgcgcaa tgattccatt gatcgacatc
     4561 ggcatggatg tccacaaagg ggcgccgtat tcgatttctg ggcaggtcgt ggtttctctg
     4621 ccgggacagc cgtgcttcag atgcttcggg tttatcaacg aaagagatct tgttgatgaa
     4681 ggacgccgtt atggtgaagc cgggggaagg ccgcaagtgg tatggtcgaa cggcgtgctc
     4741 gcatcaaccg cagtcggact ctttgtgcag atattcactc cttggtgttc accgcgccct
     4801 ggctgcgtgt ttgtcggcta cgacggggac gccttcacgt tggagcacga tggccgacaa
     4861 tccattgttg gtgggcggca atgcagccac tttgctgcaa tcgacgatct gggtgatccg
     4921 ttttggaggc cctgacgccc gccatacgga cttgtcttcc ggcacttctc gccagggtac
     4981 ttctcggcga gttggccaca gtgggcacat ccgtaggcaa tgcagagcgc tccccggcgc
     5041 gcgcgcacat ttcccttgac agccgtgtcg ccacacgcga cactcgtttc atggaagacg
     5101 acactagttt cattggcgag gtgattcgca tcataaacgg agcccttcgt ctcgatatag
     5161 acaaggtccg caactacacg gcttttttgg cggaaaagct tgaaaaggct ggcaataaag
     5221 acgccgccaa tcgcctgcgc aaggtgctag aggagggcga ccaccagctc cgtcctgccg
     5281 gggccagttt cgccagaact ctgccagttg attccgaaag ccggttcgcc ctgctggaac
     5341 gtgtcaattt gaaggcgctc tcggagccac cggtcatcct ctcacaggag cagcgggatg
     5401 tcgtccatga atttctcagc atcgccaaga gtcatgccca actcgatgtt gatggaagtg
     5461 ccctctccct cggcctgctc atgtacgggc cgcccggtac cgggaaaagt agactggcgc
     5521 gactcatcgc ccaggaactc ggcctggatc tgtacatcgc tcgactggac ggtctgatct
     5581 cttcgtttct cggcagcaca tctaaaaaca tccgcgcgct gttcgatttc gccgccagaa
     5641 ctccctgcat cctgttcctc gatgagtttg acgctatcgc aaaacttcgc ggtgacaacc
     5701 aggaactcgg agaactgaag cgcgtagtca acagcttcat ccagaacatc gacagccttg
     5761 ggaaccaatc catcgtgatc gcggccacga atcaccagga actgctcgat tccgccattt
     5821 ggcggcggtt tggttatcgg ttggcgctgg attttccgaa cgccgactcg cgcaaacaaa
     5881 tgtggctgca attcagcccg ccgcaggaat ttagtccgcg cgaaatcgaa cttctcgttg
     5941 atctctctga gggcttttcc ggctctgaca ttcgcgacgc atgcctgcga ctctatcggc
     6001 gccgcatcac ggcccagaaa gccccggatc tgcgggacgc tttccaggtt cttcggaacc
     6061 tcgcaatcgg tgaaggggag gggcgccgct ttattactca gttccagacc aaggatcacc
     6121 atgcgctagc cgccgcactc cgtgggcgca accccaggct gtatagccat gcggcccttg
     6181 ccgatctact cggcgtctcc aaggccacct cgtatcgttg gtccaaagga gtgaagagca
     6241 atggctgagg aaacgagacc gccgcgccac gaacggctac cgattaaact cattatgccc
     6301 aagcagggca cggagcggcc tgtccgaggc ggccgcacac cgccaaggcc gttccgcacc
     6361 gtcgataccg agtatcggac ccgcttgtct aatcaactct cggcccttcg cgatgtcgtc
     6421 gttcctcaga gtcgccagac tggcggcgca ccagcccgtg tcaaactcct ctcaaaggct
     6481 gctgcaaaaa gtcaccggcc agagcattta ttctcgccgg actcttgccc gattatcgga
     6541 tctggtcggc ttggagaatt attcgtgaag gccacgcctg aggggcttga ccgccttgcc
     6601 gacatcatca agcgcaatca ctccgaccgc atggtcaaag agatctcttg tgtcgaagcc
     6661 atagaaccgg tgacacctct ttttcgccgc ggcggtattg aagccaacga cgtcttgcgt
     6721 cgcagccagc gcgggaagaa aggatttatc acccgagtgc gcctgttcaa ttttggcggc
     6781 gcgggcgacc agcagcgagt cgtaagcgat ttcgaaacca cgtgccgaaa gcatggcatc
     6841 cgcgcaagcc gaaacggtta ttcaccgtcc agcttcacct acgcggccga gtgcggagat
     6901 gttggaggcg ttgaggccat ttcccggatc gcgggcgttc gatctatttc gtacatgccg
     6961 ctcattcgaa ccgtgcgccc gcgattgctc aatcataagc ccctgccaat cctcccaagt
     7021 cgtgcagagg gcgaaggtga tttccctgtg gttgtggtcg ttgacagcgg tgtctccgac
     7081 ctgatgcctg ctcttgaaag ctgggttgtt ggccgcgact cgcaggttgc gcccgaatac
     7141 cggaacaccg atcacggaac cttcgtggcg ggcctcatct gctggggtag caaattgaat
     7201 ccaacccttt ccgggatcga caacagccct tgcggaatct ttgaccttca ggtgatccca
     7261 aacccagatc cggccaaagg agacactctg gcactgctgg agcaggagct cctcatatcg
     7321 ctggattctg cgcttcggga acacgccaat aaatacaagg tctggaattt gtctcttggc
     7381 accgacgcga tctgctccct tgacgagttt tcggttttgg ctgaagagct ggacaacctt
     7441 caagaaaaat accaggtatc gttcgttatc agcgcgggca attacgctac cccgcctctc
     7501 ctcgacttcc cgcgcacggc ggaacagatc gacgccggac gaatcacggc gccagcggac
     7561 agcgtactcg gcatcacggt tggttcggtc tcgcacgttg gttacaagga gagcggtccg
     7621 agagaacacc atccgtctcc cttttcacga cacggcgccg gtcccaacta cgtcattaaa
     7681 cccgatctgg ttcactatgg tggctcatgc agcacggatc tagcgcacat cgccggcatc
     7741 cgttccattt gtggtgacaa ttcggccgag aatctcggca cgagcttcgc ggcgccactg
     7801 gttgccagga cgctcgcaca tatctaccac cagataatgc ctacgccgac tccggtgctg
     7861 gcccgcgcgc ttttgacaca ccatgcccgt gatccgcgca cccgtcgccg tgtgccggat
     7921 ggcgatgaga atttctttgg gttcggtctc cctgcgcccg ttccgtactg tctggaatgc
     7981 acgccctaca cttcaaccct ggtattcgat gacgtccttc ggcctgggta cttcctggag
     8041 tgggacgact ttccgtaccc gccctccctg cgcaggaatg gcaggtattt tggcgaaata
     8101 tggatgaccg tcgcgttttc tccggtgcgc ggctcacgat ggggaacgga atattgcgag
     8161 actcacatcg acgcgcactt cggggtgtac cgagcacaga agtcccgcga gaccggtgcg
     8221 gtcaaaacga agttcacggg cctggtaccc cccgagcaca agaatgttgg aattctatac
     8281 gagtcctatc aggtggagaa gctgcggaag tgggctcccg tccggaccta ctacggcgac
     8341 ctcggcgaaa agggggagcg cggtaaccgc tggcgattga agcttcagct tctcactcgg
     8401 cacggcatcg aggaagaaga atccttcaag cctcagccct tttccctgat tgtcaccatc
     8461 tccgatcccc agaaaaaggc cggcgtctat gacgagatgg cgcagaatct gcggactcgg
     8521 ttccaagccg aaaatttgac aatccgtgcg gctgcgcggg ttcgagcaca gaggacacca
     8581 acggtacaca aatgagcaaa accaaagatc aactgttcgt cgagcgccgg caacaaggcg
     8641 attatgcagt acggcggggt ggggcagaaa gggcgagtgc aactgctccg acccaagcag
     8701 aggcaatcga catcgcgcgc aacctcaatt cgggcaaggc accgttagtc gagcgggtgc
     8761 gcgatacaaa gtttggccat cgcgacgaat ggcgtaaggc atgatcgtgt gtgcgggata
     8821 ccgcgggccg ccttggcgcg gcgatgcgct actccgtacc aacatcatcc ggcatgccca
     8881 aatcccgtag cgccgctttg gtgtcgtcca ccttggactt cggaattccc gcaggcgggg
     8941 caacctcgac ctgaatcttg atgttcagcc cagggcctgt cgcgaagcgg gatagcacct
     9001 tggtgtagaa gttcatccac ttctgcggcg ggatctcgcc ggaccagtgc aagctggcaa
     9061 cgtcgccgct ggccgatggg gcgggggctt gatcgccagg aaggacacct tggtcgttgg
     9121 cgcctatcgc gccgaggctc ttcgcaccaa ctgctgattc gagtccgctg ggaaaaagag
     9181 tcgggccacc ttccgcggtc gtccccgctg ctccggcccc cgctctttgc gccaaatacg
     9241 cctctgcacg ctcgcgggtt atcaggtaga cgtcatcgga aatctcgaca tcgctcgcag
     9301 gcagcgagcg tttgaagacg aacggggcgt agtgtccgtc ctcggccttt ccgacgtacg
     9361 cgagtgttcc ggcatccaac ccccgcgaga tcgtttcctt cactgcgtct ggcttgagca
     9421 ggcgtggaag cgttggcgaa gcatagaagg cgtcccggac ccccttcgtg ctccattccg
     9481 gcaaagcggg aggccaggtg cgcacaagta ggtttggact tacaccgtca gcgacgatgt
     9541 cttcctcgct gagccgagtc aggatcagct ccgtcagcga attggcggag ctggagtgaa
     9601 ccaagccgag atcgatgtgg cggagatcgt tgccttcggc aaagagaaag atgtgccggt
     9661 agccgcgcca cacgatctct cgcatgtccc gttcagcccg cttggcgttt tcgcgtagct
     9721 ggcgttgctg ggcttcatcc aacttcagac tgtcggaatc cgcctcgatc tcttcccatg
     9781 caagttgctt gcgcaccgcc tccacgagcg gagccccgtc gtcaacggcg gcccagataa
     9841 gcgcggtttt gaaagttcgg gatgacgatc cgctctcgcc attcatcccc agaatttgct
     9901 tcttggttgc aggatcaaga gcatgcatag cagaaggaac cacaaccagg gccaggaccg
     9961 gccgatccgg gatctctccg ctcttggttg gaaagtagat tctgtcgatg ccgggagttg
    10021 tagtgctgct cgaaccaggg cggacgccga aaacgatgcg aatctcctgc cttacgcgct
    10081 cctcgatggc cgttgtgtca atagccgccc ggcggtccgc gaggagcttg ttcaggttgg
    10141 gctgaaaact aaagcggtag cgattgcgct cagcgctgag aaagtagcct gtatcggtta
    10201 gcgattccaa gcactgctca atgttgccga tgtccaggtc cggctcgcca accgcgaagc
    10261 ggatttccgg aatggtcgcc tccgcacgtt gctggccccc attcgactca aacaagatgc
    10321 tcgttgcgat ctttcggtga agccggcgct tcttaatttc aaccgttgcc tcctcgtcaa
    10381 ggcggaccgc atgcgaatcg ctctttcccg cgatgtccgt cgttaccgca gcctccagac
    10441 gggcctcgcc aagctgctcg aagagggcgg cgcggaacaa ggaatcatcc aacggggcgg
    10501 tccccaatgt aatgagaggg tctttgtgag ccccgctgta gccatcgcta tacgcccgtg
    10561 aaacccaaag cgcgagaatc cgtaagacgc ctcgcgtttg ctggaaccgg ggcagcgtct
    10621 gccacttgcg ttcgaatacg gaaagcagcg cggggtgaaa cggataggac gccttcaata
    10681 attcccggga gttttccgtt ggaaaccagc ctggcagttg ttgcttatgg gcctggagcc
    10741 agtcttcgta ttcctggagg gtagcggcgg cgtccttggg gatgccgctc cactcgaaga
    10801 gccgccgccg gatgatctcg gatgtttcgg attcggcgga catgatcacc gccttgccca
    10861 gccggtccag cagctttttg aagcgttcga agtcggactg atcttctgcg gtcatctcca
    10921 actccgacgc cggaattgaa accgccagga caactttatc tcgggctcgg gcaacctcgg
    10981 agaggttgtg caaaaagcta taaagctggg cagccatacc gctttttcgg ctgcggccaa
    11041 cgtagttcat cacctcgtcc agcaagatca gcgatgcgcg tcccgccggc aggagcttct
    11101 caatgacatc tccgcctggc gcggttagcg atctgtcgtg ttcctcgacc agactgaatc
    11161 cctcgatgcc gccaagctgg taggcgatct cgccccaggg ggtttttctg agcggcgtgc
    11221 catccgcgcc gccacgaccc gtgatcgaat cgaactccgt gccgacaaaa acgccggtcg
    11281 cagcgcttgg aaccgctgtg actcctgcat cctgcaaaag gccagagacc cctttccacg
    11341 agcgcgcctc ggcgccgccc cgcgccaggt ggtaaagcag cgtcagggcg tgagttttgc
    11401 cgccgccgaa ttgcgttgac aggttaaaga cagcggaggt ctcaaccttc attcccgaga
    11461 gccgccggac cacttgcatg gcaaggtcgc tcagattttt ggtcaggtag gtacgatcaa
    11521 agaaggcagc cggatcctga taaacttcgg gagcgcgccc atcgcgaatg tgatcgagat
    11581 ggacggcgaa ctcggaagcg tccagaggtc ggccggagcg cagatcctct cggggggtga
    11641 cgactttgta ccatggtttc aaagaaggca tcgctgtcag tcccccgcag caccggtgtg
    11701 atccttagca atttcttgcc aatgatcatc caggaaaact acccggtcgg catagctctg
    11761 tagatcggcc gaaacgctat tctggaaccc agcaatccag atttccttct gctgcccgct
    11821 cttgacgtaa tcaatcgcgt cttcaaaatc gccatcacct gccgatagca ccaggcgctc
    11881 atattgattc cgtgccgcca acttcaaaat gagcgttgcg atgccgacat cgacaccttt
    11941 ttgcacctca cgtatatcgg tttttccgca ggaactacac cgtgtcgaaa cattctttag
    12001 tttgtaaagt tgcacccgca tctttggccc tttcgggggc gcgaacttca accatgtatg
    12061 gaaggcgtcc tgcccatcgg ttggcggatt tggcgtcgag ttgaagtaat atgcctcggt
    12121 gaattcgccg ccgttttccc ctgccagcag aactttgagc ttaaggtagt caaatttccc
    12181 cggcgaggcc ttcagtaaat acgcagcatc gacaatccag atcgccctct tttggtctgt
    12241 cataagccga gtcctttctt gcgcgctagc acgccgtcca cccagcgctt ctcatccgag
    12301 ccggcagggt acaaagctga caaggcctgc gccagccgcc agaagcggtc atccctcccg
    12361 actccttcat cgattagaaa ttgtttgatc gcttcagaac ggcccgctgc aaaaaggatc
    12421 atggcttggt ggacccggtc aagcacggtc gccccgagcg taggcgcgcc ttttacgccc
    12481 caactgcctg ccccctcggc ccgctccaat tcggctacga agccaagctt aagctgtggg
    12541 tccttcttct tgcgaggctt ggaggccgcc ggaccatctt cgtccttgcc aaacagcctg
    12601 cgtgtccgct ccgcgaccgg cagcagtcgc gccgtatcgc ctttaacctc aacgagcgat
    12661 gtcagtgctt caagatgagc gcctaggcct tgggcgatct tgcgcgctgc gtcatattcg
    12721 agagcgtagc ccgttgatat cgcgggtgtc acatcctcgc tgtcgtcgcc agcctccggg
    12781 tctcctccag tgccgtcctc tgctgtcgtg ccgttccggt tggcggaaag cgcccatagc
    12841 cacatggcgg tcaagcgcgc gtcctcttcg aagccactcg catcggcgcc tctgaacact
    12901 agggcgagcg cctcattcgc cactgcggcc cacacctgca ctaaatattc gccgagcgta
    12961 acgggctcac cgctagcttt ctccacgcgc gaatagcggg aaaatatttc tagtgccgga
    13021 ccgaggcatg caaagatggc atccgcgccg acgacgcctt cctctgcgag acgcggcatc
    13081 cattcgtgta tccggctcgg caactcgttg agcacattgc gccaatcgcc gacttcgttg
    13141 cctgcccctg cggcggtatt ctcgcggggt cggcagacga tgtgaacgga agaggccagt
    13201 gacgccgttt tgagcgcatt cggtcgagcg gcccgctctg tgtcaattgg ccaagaagcc
    13261 gtgacaatcc aaccgctatt gatgatggct tccaggagcg cctcccaacc ggaggttgac
    13321 ttatgagcaa acacgatcac cgcgattccc gaaaacgcgg tgaagtctcg gcctttggag
    13381 aacgcctgtg tcagtagttc ctcgtatcgt tctttttcct cctggctgtc aggatgattg
    13441 acagtcatct cgcggtcttt ccttaaattc tcaagccgct cgattccgtg ggtagtgcgc
    13501 tttagccata cgtaaaagta gtcactcaga tcggcgtacg ggacagaatc gtagtagggt
    13561 gggtcggtta tcaatgcgct tgcagcgcac gagggaagcg ggcattcttc ggcggacgat
    13621 tggcagattg ttcctgtttg tgaagatgcc gttgcctccc gtgagatgag cctggcgatc
    13681 cagtcaattt gattgtccca acttcccgag cctgagccga gcgggacaga ttccgcataa
    13741 tctgaaatca tcggtagctt tttttcaccc gccagggtgc ctgcaataaa ctccccgctg
    13801 gggacccagc ggcaaagcga cgaggagtag tctgcttgct tatctaccgc aaatgctagt
    13861 gtcaggaaga cagcgcgggc aaaatgctca tctaaagccg taggtagcaa ttcgtacgcc
    13921 ctgcgcaccg cgcgttgcag cgcgaggatg gttacctgct gcctggcagt gaataagctc
    13981 ccccaagtgg aaaacccgta aagcggaagt tgcgagccca cggctctgtg cgcgcccttc
    14041 tggggcacct gctcgtcagg tattacgcta ctgcttgacc gagctgcgtt cactattttt
    14101 tgtagtgcag ccttggaagc catgacggcc atctcgtcga gtggcccggg ctcgcgatat
    14161 cgacggccgc taggccccgt ggtcaggacc gcgacaagac gagaattgtc tgcgccgcca
    14221 ttctgcgcgc tcaattggcg ctggacgctt tttttgggtg tagaaaagcc acatcccggc
    14281 agcggacaag tcgctacaaa acctctgcag gtcgctttcg cgatactctg atcatctgct
    14341 tcgaccagag atatcgcgac acctttggag ccatcgctcg atctgaaggc tatccccacc
    14401 cccccttgtt ttcgctgagc gatcacagtc cggccgatca gcggcacctg cgcaccgcag
    14461 cctgggcctt cgcaatggat agtacgcgcc cacaaatagg ctatcggagc tgcgccgtct
    14521 gggtccttgg ggtagagatc agtcagttct ttctcaacct cacgcttgac ccattcaccc
    14581 cacttgcgga tctcatcggc gaggcgctcc ccgtacttag ggatgtactc tagaaccacc
    14641 ttgttgagca agaccgcgac cgggttaagg tcacttgcga aagcgtccgc tcccacgcga
    14701 agggcctcca gcggaatcga gccgccaccg gcaaaagggt ccacaacaag cggtcttgtt
    14761 ccaggttccc cgccgagccc ctcgtgtgct gcttgcgtaa gcgctctgct ggtttcaaga
    14821 taagctggga ccgtcgaatt atcccaattg gcaaaatccg cgatgaaatc aaggagagcg
    14881 cgtcgaagct cggtgttatc cagtattttc cccggttcct tgcgtagcgt tagaaaccgc
    14941 tcctggctct caaggcccag cagcttcaca tgatccagcg cccactttgc catctcttcg
    15001 cgggctattt tccggaaact ctccgggcag agtgcgtctg ccggatcggg ccaaagcgtg
    15061 gcgcagatca ctgcccggca cgatgctagc ggacggcgcg cccaccagat gtgcagcgtg
    15121 gagatgtgcc cgggacggat cgacttttcc cggcgcgcgt gcgccgaaat ccgcttgatc
    15181 ggaagatcga cctcaatcag ccgcttgggc cagcaacgcg gagccacagg aagttcaata
    15241 ctgcgatcgc tggatttctc gccgcttgtc gccgcaatga cgccgctcgc cgacggaaca
    15301 tgcgtcgact tcacggtcaa cggcttcgat cccgcaatgt atccagttcc ggatgatcgg
    15361 ggcggggcat tcgtccggcg acgcgcctct accgcgccga tcggctcatt gaaaaggaga
    15421 aactgaggaa ttgacggcgg ctctacccgg cataattggt cccgatctgg cccgcgcagt
    15481 gcttcccgaa gctccatcaa taggcgaccc agaacattgt ttcctattag gacgtcctct
    15541 tcggcgggct tcgcacccca aaaatcgtcc tttcgggaat cttcaacgat cgggccatca
    15601 cccgttccga gcagcaaacg cccaaaggtt tcccaattct gggccagctt gacccgaagg
    15661 caccagcgca tgatctttac ccggactctc acccaatcgg accgggactg gccacgaaac
    15721 ggcttcgact tcattttcgc ggtcattggg ctcttttcgc tgatgatgat gcgctgaacg
    15781 tccggcagat ggggaaatcg gcatgcctga tagagcgcct cagaggttcg aatccgctcg
    15841 ccgtttattt ccaatgggaa cccaccagcc atgttcgata gccctccgaa ttcctcgtct
    15901 gttttacgaa aggagacgga atcggcccgg atgtacgtcc tctgctcggc gcggcccatt
    15961 agtttgtttt cctcgggaat agtgggtacc acggatctcc agcccaaaac gccagcgggc
    16021 agttgttcgg gcagttgcgg aacgtgacga tcgttgcccc gaagcccaac gatgcaagca
    16081 ccatgttgcc aagtgggcgc tggtagatgt taaggtaggg gcattgggaa cggatgcgca
    16141 gcccggcctt taacagctcc tgttccaaca ggtttcgtcc gctatccgag aagaagaagt
    16201 tattttcgcc gagcaatgtg ccggtgcgaa caagcggcgg gtgggtcaga gactggacgt
    16261 acgcctgtgt gagatcatcc gtcgggatgg aggcaggacg aagcacgtcg gcgtaggcgt
    16321 tgcgtgcctt ccgatcctca atctcgagat gtctccaaaa ggtcacgtca atcgttttgc
    16381 cggcggctgt tgacgcagcc gcaatccccg ttttggcgta atagttgccg ccccggtgga
    16441 aagcgatgac tataacgtgg accttcacat tggccggagc agaggagttg atccagttcc
    16501 gaagatcgct cctgacccgg ttacccgtga agatcacatc atcgaggtag acgtagggac
    16561 cgtacgccag cccgcactgg tcaattctta ggccgcacac atgctttaat tgttggctaa
    16621 acatttcccg catttcgcgc tggctacttc caccaccctg gatatccaaa aaattcgcgc
    16681 tcctccagaa atcgcatggc gacgcgccga caagttgtcg cgactgaagg acggacgtaa
    16741 gaaaagcctc aacctttggc ttgctgatgt acgtcttctt caggacgtgg tccaactctg
    16801 cgagcatcgg ttcctgtact ggctgctcga actggccgat ccacgtcgct acatggtttt
    16861 cgtctagcgg cgggatctcc ccgaggcgat aatccgcgat cgtgcttgcg acggcggcca
    16921 agagtgcttt gcgctcgcta ctcggcattc tctcctccga ggatctcctt ggcacagaga
    16981 tgatagtgcg cgacctgaac tatcggctgc cacccgagcc gcgcaggatc gcgaatcgcg
    17041 tgaagttctg gcttcgttcc gcagttgaat accacataaa gccaatagtc gttctttaag
    17101 cgctcagcgg ttctgtactc gttggcactt aacgcgacct cgcccacgcc ggcgcgccct
    17161 ttcacttcta taaagcgtgc ggcggcgaag acacctggct cggtggggtg gggcttgcgg
    17221 gagagcagat cgtatccccg gttctccgtt tcaacgcttt cgactaccca accattctcg
    17281 cgttcgtgtt ccatcgccac ccgcacggca atcttttcga tctcggcatc ctgcaccatc
    17341 ggcgcaatcc ccggcgcgcc acgatcgggg tgggggagca cccaggcttg gcccaggtgc
    17401 tggatgtcgc cgatcgtgca atggcgctcc atgtcaagct ctgaccggcg gcgttccagt
    17461 cgtgcgttca gttcatcaag atggttctcc gcctggacga tgttcccttc gagtccggcg
    17521 gcgcccccgg aggcgattcg gcggttgagc agatccccta aaacgttctg ctggcggtca
    17581 atcagagccc caagggagat ttcaatgtgc tcgcgaacaa tccggttctc agcggtgcgt
    17641 tccgcgccga tctcgtctcg gaatggttgg agagtcgttt gaatgaggtg cacctccacc
    17701 gctgtcttat cgggaagatt gtgcagggcg ggctccgttg tgcccttggg ggtggggatc
    17761 agatcgagga aaagcgttgg ctggcgggcg gttaacgttc catccgggtc cgtgcgaacc
    17821 acgaatagcc gcttgtgaag ggtatttccg cggccgtctt tgatggctgc ggtaaaaaca
    17881 tccagccggt agggctcgct cgtctgcagg tcccacaaga ccgttccgcg gcgaagatcg
    17941 ttcgccacgc ggtcccatac ctccgtacgg accgcctcaa acagcggatg cccgggcgtt
    18001 acccattcaa gcgtcgcgtc gtcgccaagg agccttttgt caaagacgac gcgctggtat
    18061 tcccgtccga gtttcccgaa tcgtggctcc tggcgttcgc caattgcgct cagagatttc
    18121 gagaccttac cgatccggta gacgtgttct ttcccacgaa cggcggcggg atgcacgctt
    18181 gcaacgggtc ccccagcctt gaaaaagtct tcgacgactt ccggaactag gcggcgctcc
    18241 tttgcctcaa cggatttgcc taccaaagcg gagaggttca agtccttctt cgccaggccc
    18301 tccagcgtag cgcctgtgat atggcgaaac cgctctgggt cgatgtcgcg gacgatccgg
    18361 tcggcgatgt tcgattcgct cgtccgtttc gcgtacatat cgcgaaacat cttttcaagc
    18421 tggttcgaag gtagcatctg tccaatgacg tcgaagactt tatcgctgcc aagctcttcc
    18481 ttgatctccg cgaggcgaag gaagagcttt tccagcacgc ggccctcgcg tgtgttcacg
    18541 gcggcgaaat tgaagatcag acaatcgttt tcctgaccgt atcggtgaat gcggcccatc
    18601 cgctgctcca gacgcaccgg attccagggg atgtcatagt tgatcatcag ccagcagaac
    18661 tggagattga tgccctcacc ggcggcttcg gtcgccacca gaacctggca gtcctcgcga
    18721 aactcgcgct cggcgtagat tcgcgtgccc ggcgtatccc gatcaccgat ttgcatgccg
    18781 ccatgaatct gggtgacgct cagcccccac tccctaagct ttcccagcgg ccggccatca
    18841 tgcccatcgc cggcaaggta gtcgagggtg tccttgtgct cggtgaagac aagcagcttc
    18901 atgcgcggat cgctgaagat gtgctgttcg ttcaggacct ggcgaagctt cgtcagcttg
    18961 gattcgatct cgcgctgttc cagcgcccgg gcttgttcga tgagtttttc cagcctttgg
    19021 atctcatcgc gaagcgagac cgggtcgatg attaggaccg caccctccgc gtcggcgatg
    19081 attttggcct gctcatcttc tggaagttct tcgaaatcgt ccggcagttt cttttcgatt
    19141 tgttcccggc ggtgcttttc cgggtcggcg agaatcttgc atcgcttatc gcgcatgcgc
    19201 tctaagcttc ggcggactgc gtagatgcaa gaggcaaaac gccgctgcag catcgccatc
    19261 tggaaggaaa gaatactggc ctgaacattt tccgccgccg ccgccttgat ggactggtcc
    19321 tcaacatagc gggtgagcgc atcgtaaaaa tcgaactcgt ctccatccag ctcaaaacgc
    19381 gtcgtgcgaa cgtcgcggtt ggtgaagagc ttctttacct cgccggagtc ggggtcggga
    19441 aatgtaacca gcgcttcctt gaggcgacgc aggtaaaagg gtgccgagtg gcgacgcatt
    19501 gcctcctcca gactcttgac gtcaccgtag acgtcgcggt ccagaaggct gaggaagagc
    19561 cggaagttct cggtatcgcc cttgtggggc gtcgcggtca tcaggagcag gtggtcggtg
    19621 cgctgggaaa gcgcttcgcc gagttggaat gccagcgtct tcttgtcgga gctgtaggcg
    19681 ctcattttgt gcgcctcgtc cacgatgacg aggtcccagt tcgagcgcaa caggctctct
    19741 ttcgcgtcgt ccacgcgaga gatccaggac acagaggtga cgatctggtt gcgctcctgc
    19801 catggatttt ggccgtactg ggaacgcaac acctcaccgc gcatgacgtc gaactgttcg
    19861 cgaaacttgt ccctcatctc ccgttgccac tggaaggtga ggttggcggg cgccacgatg
    19921 agcacccgtt tcaccaggcc gcggattttc agttccttca gcagcagccc ggccatgatc
    19981 gtcttgcccg atcccggatc atcggcgagc agaaagcgca tgcgtggcag ctttagaaaa
    20041 tagtcgtata cggcttcgag ttggtgcggc agcgggtcaa cgcgggccac ggaaagggaa
    20101 aaataggggt cgtactcgta ggcaagccca aggcgagcag cctcgatgcc caacttaaaa
    20161 tgcagcgggt cgccgtcgaa cggcgcgtcc ttcggtgtta gggcaagctg agccacctgg
    20221 acttggtcga gaattggctc gtgcacctgt cctgtgtgga ggcccttgcc tactaacttc
    20281 acggctgtcc ccatcgagac gatagccagc acctcaaccg gctctgggag aatcggcccc
    20341 tcgacgaccg cacctggaca aaggtcctcg aatgtagggg gcgtcatttg ttctgcctct
    20401 ggtgcgcggc cgcatgcgct tgctccgtca tccaatgctg aagatccacc ttccgaattc
    20461 gccataggcc tcgtactttg aatgccggca gctcgtgcgc gttcgccatc gcgtaaacag
    20521 tcttgcctgc gagcttgagg atcgccgcga cctcgcgaat ggtcaaaacc tcctgcgcgg
    20581 ctggcgattc gatccccaaa ggtttactgg tatagcgcgg cattagactc cacgtcacgc
    20641 cattgtactc caagatggta tcgagcgaag tttatcgttt ttcgctaatc agaaccgcat
    20701 ttccactgcc cgccaaagcg gtcgcgctga ctgcgcaact cctcacgcga tctcggctcc
    20761 ccgtctggcg ctgactccag ttgatctcgc ccggtgactg agaacggtgt gctctccccg
    20821 cccctgcgcc ctttggtgcg gcgctggctc acgcggacaa ccgccgcgcc ctggcggcac
    20881 tgcgggcttg tgctttcttg gctcccccaa aacgctgcgc tggtcccccc caagaatgca
    20941 gcccttggcc ctgggagcgg cccgcttcgc cctgggcgcc ccgcaccaaa agcttcgggg
    21001 cagaaaaagg agcacaccat gaattctcaa atcatcaccg ccgagtccac ttccaccttc
    21061 cagccccagc ccgacccgcc aatttcggcc gagaccacag gcgccgcgcc cttgtgttcg
    21121 atgtggcgca gacggtgggg atacccctgc cgtccgcgcc gcaggcaaag ggcgatccag
    21181 gggcaaacat tgaaggcatc aaggcgttgt atgcgcaaaa ggtattgcgc tggagtacaa
    21241 ggacaacctg gacggcgcgg agggactgag cgccaagggt gaggtctacg ttctgggcgg
    21301 cctggaaccg gcgcgaattc gccgttctgg tgcgtgcatg aactcgccca cgaactgctg
    21361 cacggcggcg aacggcggaa gcagctaagc cgcaaggtca aggaaacaga agccgaagcc
    21421 gtggcctacg cggtggggtt gcccgttggc ttggatatga ataccgcttc cgcccactac
    21481 attagcctct acgacggcga ccgcgacacc ctccgcgaat cgctcgattc catcacccgc
    21541 gtttcctccg aaatcctcca gggcggcggc gagggtcagg gcggcggcaa gcagggggcg
    21601 cagcatggcg tggctcaggg cagactgcga ccccgcaccc caccgtatac cggcgggggt
    21661 atactgtcaa ctgactggcg cgggaaccgc gcagcgaggc atccatggcc cacaccaaag
    21721 acgagaagaa gcgcctcctc caccgcgtcc accgcattca gggccaggtg gctgcga
//
LOCUS       DATNDT010000337        14144 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_926865, whole genome shotgun sequence.
ACCESSION   DATNDT010000337 DATNDT010000000
VERSION     DATNDT010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 14144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14144
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926865"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1083
                     /locus_tag="VN788_10845"
     CDS             172..1083
                     /locus_tag="VN788_10845"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXU21069.1"
                     /translation="MKRTSTILLALGAALLLLIGVVTLVRARRILAANHAASSHSVAA
                     HAKSAQETGDDADDSGVPSVIHFARNPERMPPFLVNDLDGRIISTADLHGKVVLVNFW
                     ATWCPPCREEIPELIELSKRYKDQLQIIGISMDDAPAGQVRQFAKQIGITYPVVMGSG
                     AIEAEYGGVPALPTSFVISPEGRVVQKHAGLYPIGVYDTEIRSLLGMPVKAKVETFQD
                     VGQIFMKNAANATELPGVDFKGLTAVQKRDALKRMNTDLCTCGCKYTIAQCRINDSSC
                     ATSKALAAKIVHEIAANRPAATRAAAN"
     gene            complement(1108..1977)
                     /locus_tag="VN788_10850"
     CDS             complement(1108..1977)
                     /locus_tag="VN788_10850"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXU21070.1"
                     /translation="MRGLISGAILTAIFLVIPSVSGARQSKIVWNDQEKPIAQQIGTL
                     RSLPDDLRARTTKQLALQIRQLPAGAHKPALANALANLSTEGDFGRDTLQEVTTTLAD
                     ALREHPVPEEKGQPAPPYIELAQLVRYEHMRAASDSLEFSAAMAKLKSDDERRQHADF
                     TLTDLKGKSWRLSELRGKVVVVNFWATWCPPCRKEMPDLEALYNRFKDQGLVVLAISD
                     EDAAKVRPFVAERQVTYPVLLDPGRKVNDLFQVEGIPKTFVYDRDGKLVAQSIDMRTR
                     KQFLGMLVEAGLK"
     gene            complement(2071..2547)
                     /locus_tag="VN788_10855"
     CDS             complement(2071..2547)
                     /locus_tag="VN788_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HXU21071.1"
                     /translation="MAKKSAGLLMYRVRDGKPEVLLVHLGGPFWAKKDAAAWFVPKGE
                     VEPGEDELVTAKREFKEETGFDPGERLQPLGSVKHKSGKRVTAWAFEGDCDPAALKSN
                     TFTIEWPPRSGKQREFPEIDHAAFFNVEEAREKIHPAEFEFVARLNSLLSGQGTKV"
     gene            3012..4508
                     /locus_tag="VN788_10860"
     CDS             3012..4508
                     /locus_tag="VN788_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HXU21072.1"
                     /translation="MVEKLVDAAGLLDCIYWRQSDPDGLKLYLSLAKSKNPQDQMLRE
                     YLKINGGRYDLINDNKPFVGTQPMPPGRGFYPTDLTRSAFDAYVAAHPAQKAALYSGQ
                     TIVRRKGSALEAVPYHVAFHEFLVPMAADLRAAAALSDDPAFAKFLRLRADALLSDDY
                     YPSDVAWMDLDNPKFDVVFAPYEVYLDGLLGVKTSYGASIMIRNDAESKKLAAFQKYV
                     PDLQESLPLPHEDLPSERGQRSPMEVVDAIYRAGDLLHGYQAVADNLPNDPRIHQEKG
                     SKKIFWKNFLDARVNYIILPLAERLLPANQAKMVTGEGTLDFVILHEISHGLGPTYVH
                     GSNGKQHIGEAIGPRYSALEESKADITGLLCVKWLVDHGAIPRGKLNEIYASFLGETF
                     RTIRFGIAEAHGAGAMMELSYLTEQGAVRRNSSTGLYEVDFAKMPTAIASLAKELLEQ
                     EATGDRSRTEKWFKKYAVMPPQLSAALAKGSDIPVDVDPQFDFHPPLR"
     gene            4601..5089
                     /locus_tag="VN788_10865"
     CDS             4601..5089
                     /locus_tag="VN788_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU21073.1"
                     /translation="MAKVKPVPEGFHSVTPYLAVDDGARAIEFYQQAFGAKERMRMPG
                     PDGKISHAEITIGNSIIMLGPPQANTGVRSPQSLGSSTVSIFLYLDDVDSTFKKALSA
                     GAKEAQPLADQLWGDRYGRVTDPFGHSWSLATHKEDVSAEEMSRRMQEAAKTQHRAQT
                     AG"
     gene            complement(5186..5470)
                     /locus_tag="VN788_10870"
     CDS             complement(5186..5470)
                     /locus_tag="VN788_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21074.1"
                     /translation="MEWMAMQTPDSNAKIELNQVVQKYLGAAGSYGHPVPLDALGLSA
                     KEVEDVFSTFDEDYHISRFFHFECASGANYQINGFPQTHVSIDAGIQSIL"
     gene            5731..6204
                     /gene="rimP"
                     /locus_tag="VN788_10875"
     CDS             5731..6204
                     /gene="rimP"
                     /locus_tag="VN788_10875"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HXU21075.1"
                     /translation="MDLERIRQAAERVAHSEGLEVFDIEWKVGKQRLLRVYIDRLPDT
                     EHPDAAVSHNDCQRVSEQLSVILDVEELIPGTGSYVLEVSSPGLDRKLIKPGDYERFA
                     GRLAKIWLTEAVENQKYWEGRLAGHDNGIVKLAVRDRELAVPYSQIRKANLVVEL"
     gene            6241..7764
                     /gene="nusA"
                     /locus_tag="VN788_10880"
     CDS             6241..7764
                     /gene="nusA"
                     /locus_tag="VN788_10880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HXU21076.1"
                     /translation="MVSLLYQTIEQISREKHIEPEIIVAAIEDAMVVAARKYYRTEEE
                     LRSKFNPESGQIDVFSVHMVVEEVADPVREISLTDARKRNPEIEVGGEITQTKPTDVL
                     GRIAAQTAKQVILQKVREAERDTIYNEFHTRVGELINSVVKRMEGPDMIVDLGRTEAR
                     LPKREQSRLEAYNVGDRLRVTIRAVERAAKGPQVVVSRADPALVQRLFEMEVPEIYDG
                     TVQIRAVAREAGERTKIAVESRDKDVDPVGACVGMKGMRVQSIIRELRGEKIDIIPYS
                     EETVAFAQKALSPAKVTRVQIVDPEEKRLEVIVEDTQLSLAIGKKGQNVRLASKLIGW
                     NVDIKSEEEKRREIEAQMAALTAPGTALAELSGVGPKTIEKLEAAGITSIEKLADMTP
                     EQLVEIPGIGEKMVEKIHQSVAAYFEALEAQANAPIEPSTEDEAAAEAMPEASVETPD
                     TIETSDQAGTTDLTEEAQEPASNAEKPSEEDEETTAADKEAAAKVDESAEKQETPEE"
     gene            7829..7933
                     /locus_tag="VN788_10885"
                     /pseudo
     CDS             7829..7933
                     /locus_tag="VN788_10885"
                     /inference="COORDINATES: protein motif:HMM:NF016635.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2 N-terminal
                     domain-containing protein"
     gene            8663..10660
                     /gene="infB"
                     /locus_tag="VN788_10890"
     CDS             8663..10660
                     /gene="infB"
                     /locus_tag="VN788_10890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HXU21077.1"
                     /translation="MPKAEPGKPLYQRKPPASRGRPTIEKRYAEGERRLHPVRTRAGV
                     GAGRAAHAEPVAPVQREPRNVTVTEGITVRELAEKMDIRAKDLLKTLLDRGVFASINQ
                     ALDVPTATSLAEAFNGIVQVVTFEEQVVQEEKIKEERTGNQLPRAPVVTVMGHVDHGK
                     TSLLDAIRETEVAAGEAGGITQHIGAYEAHVDQRRIVFIDTPGHEAFTRMRARGAKVT
                     DIVVLVVAADDGVMPQTEEAIAHAKAANVPIVVAVNKIDKPEALPERVKRQLADRGLM
                     PEEWGGDTVMVEVSARAKKNLPRLLEMILLVSDLRELKADPDAPGSGTVLESRMDKGR
                     GPVATVLVQNGTLRVGDVFIVGAVYGKVRAMFDDRGQAIQKAEPSTPVEVLGLQGVPE
                     AGDQFQVADEAKARHIVEYRQGKQREAALAKTGPSGRLTLDQLHEQLRAGEMKELPVV
                     VKADVQGSVEVLNELLPGLSNDQVRLKVIQASVGAVTERDVLLASASNAIIVAFNVRP
                     ERKASDLAQREGVDIRLHTIIYELIDELKKAVSGLLAPLIKETYLGRAEVRDTFRVKG
                     VGMIAGCSVQEGILKRDADVRVLRDNVVIYTGRISSLRRFKEDANEVRAGFECGAGIS
                     NFNDVKVGDSLECFKIEKVAPVEAPPDRAARPQAVVEARRG"
     gene            10782..11075
                     /locus_tag="VN788_10895"
     CDS             10782..11075
                     /locus_tag="VN788_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HXU21078.1"
                     /translation="MPVGLLTLEIHISDAQSLKDKRQVLRSLKDRLRAHFNVAVSELD
                     YQEVWQRSRVGVVTISGDGKHLEESVQAIAAESERILGRDLLSSQIEYFEDGE"
     gene            11072..11434
                     /gene="rbfA"
                     /locus_tag="VN788_10900"
     CDS             11072..11434
                     /gene="rbfA"
                     /locus_tag="VN788_10900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HXU21079.1"
                     /translation="MTVGGFRAERIAEEIRNEVGLMLAGELKDPRLAVPLSVTEVRVA
                     PGMRVVRVFVELQGNDAERSKALAGLKAAAGFVRHELVERLRLRRAPEIIFLADESEE
                     YGQHIDELLRKVHHPEKS"
     gene            11489..12463
                     /gene="truB"
                     /locus_tag="VN788_10905"
     CDS             11489..12463
                     /gene="truB"
                     /locus_tag="VN788_10905"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HXU21080.1"
                     /translation="MSRIPQPPPLDGILVVDKPRGKTSHDVVETVRRLAGFRQIGHLG
                     TLDPLATGVLVLALGKATRLARFYKGRRKRYTFAVRFGFATDTYDADGEALGADAEPA
                     LDEAEIQALAAQFTGKLQQTPPAFSAKKIGGRPAHELARKNKPVKLEPVDVEVYEFRL
                     TGIDGAIARFSVECGAGTYIRSLAHDLGKLHGSGAHLAEITRTAVGEFTLDHAAPLAE
                     LVEDARSGKIAARVIRLEGLLSELPRATVLPIVEKRVRHGAKFNLPLAQIQPGHATAA
                     QGAPAELDSGEWQPSRLRVFSQQGQLIAIAEPVVPRTYQPVVVLDAAQ"
     gene            complement(12516..>14144)
                     /locus_tag="VN788_10910"
     CDS             complement(12516..>14144)
                     /locus_tag="VN788_10910"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXU21081.1"
                     /translation="SFVTTADLRANSINDLIVANSADNTVSVLLGNGDGTFGAQATFP
                     TGTNPVWITSAQFNNGTTAANSDNILDLAVANKAANTISILLGKGDGTFQPKADIPTG
                     RAPVSVTTANFHDGTGTGFLDLAVANQDDNSISIFQGNGDGTFQAPALIQLPAGFSPA
                     ALAATDLNDDGHADIVVADRGHNTISVFLGNGDGTFRNRTDYPTGAGPVSVAFGDFNG
                     DGAEDIAAANLSGNSVTVYYNQISSTTSSPLGTFAAGAMRDFAAGNGPTAIAVADYNT
                     DGLADIAVSDTADNAVTALLNGGDGLFTALPEIPVGTAPSSIATADFNADGLPDSVTA
                     DSASAQATVIINSASLFGSAGLNSFGTPYPGVQYLDIGLKVKATPRIHPDNDVTLELN
                     FDISSLSSQSINAIPVISNDEVDQTVRLKPNETAALAGFLETQLTNAITGNPGLAEIP
                     GVGLLDQNRNAQQQETELLILVTPRMVRLARRKDHTIYAGQGASEGAGGGAVENTFAP
                     QPPPQQVPEQNEEPPPPSPPPLPTQRGTQPTPDQ"
BASE COUNT         3130 a         4181 c         4109 g         2724 t
ORIGIN      
        1 ggcgccttgc ccgtctctaa tctgccgttt caattcggga ataaccctcc cgtagcgggc
       61 cgaagcagca ttcggctcac gggtagttct tcagttttaa actgaagcac cctggctcac
      121 gcctatcatt gtgcgaatca acactctgcc gcataatacc agcaggatcg catgaagcgg
      181 acttccacaa tcttattggc gctgggcgcg gctcttcttt tgctgatcgg cgtcgtaacg
      241 ttggtgcgcg cgcgccgcat tctcgctgcg aaccacgctg cgtcttcgca ctcagtggct
      301 gctcatgcca aaagcgcgca ggaaactggc gacgatgccg atgattcggg cgtgccgtcg
      361 gtaatccact tcgcgcgcaa tcccgaacgc atgcctccat tccttgtaaa cgatctggat
      421 ggccgcatca tctccacagc cgacttgcat ggcaaagtag tgctagtaaa tttttgggcc
      481 acctggtgtc caccttgccg cgaagaaatt ccagagctga tcgagctctc caaacgctat
      541 aaagatcagc tccagatcat cggcatttcg atggacgacg cgcccgccgg ccaagttcgc
      601 caattcgcca agcagattgg gatcacgtat ccggtcgtta tgggcagcgg cgccatcgag
      661 gcggaatacg gcggcgtgcc ggccctccct acgtcattcg tcatttcgcc ggaaggacgc
      721 gtcgtccaaa agcacgctgg cctctacccc atcggagttt atgacaccga aatccgatcg
      781 ctgctcggca tgcccgtgaa agccaaagtt gaaacattcc aggatgtcgg ccagatcttc
      841 atgaagaatg ctgcgaatgc cacggaactt cctggcgtcg atttcaaagg gctgactgct
      901 gtgcaaaagc gcgacgcgct caagcgcatg aacacggatc tttgcacttg cggctgcaag
      961 tacaccatcg cgcaatgccg catcaacgac tcatcctgcg ctacaagcaa agccctcgcc
     1021 gccaaaatcg tccacgaaat cgccgccaac cgccccgccg ccacacgcgc cgcagcaaac
     1081 tagctactac agaaatttga cggctggcta cttcaatccc gcttcgacga gcatcccaag
     1141 gaactgcttc cgggttcgca tatcaatgga ttgcgccacc agcttcccgt cgcggtcata
     1201 aacgaaggtc ttaggaattc cctcgacttg aaacaaatcg tttaccttgc ggcccgggtc
     1261 gagcaggacc gggtacgtca cctggcgttc ggcgacgaac ggccgcacct tcgcggcatc
     1321 ttcgtccgag atggcaagta ccaccaggcc ctgatccttg aaccgattgt agagagcttc
     1381 gagatcgggc atttccttgc ggcaaggcgg acaccaggta gcccagaaat tcacaacgac
     1441 gactttgcct cgaagttcgc tgagcctcca actcttgccc tttaggtccg taagcgtgaa
     1501 atccgcatgc tggcgccgtt cgtcatcgct tttaagtttc gccatcgctg cggagaattc
     1561 cagcgaatcg gacgccgctc gcatgtgctc gtagcgcacc agttgcgcaa gctcgatgta
     1621 cggtggggcg ggctgcccct tttcttccgg cacggggtgt tcacggagtg catcggccag
     1681 cgttgtggtc acttcctgca atgtatcgcg gccaaaatcg ccttcggtgg aaaggttcgc
     1741 cagagcattg gcgagagccg gtttgtgcgc gcctgccggc agttgccgaa tctgaagcgc
     1801 aagctgcttc gtcgtccgcg cgcgaagatc atcgggcagc gaccgcaggg tcccaatctg
     1861 ttgcgcgatt ggcttttcct gatcgttcca aacgatcttg ctctgccgag cgcccgagac
     1921 ggatggtatt acaaggaaaa tggctgtaag aattgcccca gagattaggc cgcgcatttt
     1981 cacctcagct acttttagcg acattttgtc actgagggta ttttacttct aaacgtctct
     2041 ctcccgtctg cgggtgttcc atgtcgaaag tcagactttt gtaccttgcc ccgaaagcaa
     2101 tgaattcagc cgagccacaa actcaaattc cgccggatga attttctcgc gcgcctcttc
     2161 gacattaaaa aacgccgcgt gatcgatctc tggaaactcc ctctgctttc cagaacgcgg
     2221 cggccactcg attgtgaacg tattgctttt caaagcggca ggatcgcaat cgccctcgaa
     2281 cgcccacgcc gtaacccttt tcccgctctt gtgctttacg cttccaagag gttgcagccg
     2341 ctcgcccgga tcaaaacctg tttcctcttt gaattcgcgc ttggccgtca caagttcgtc
     2401 ttcgccaggc tcgacttcac ccttaggcac aaaccaggcc gcggcatcct tcttcgccca
     2461 aaaaggcccg cccaggtgga ccagcagaac ctctggcttt ccatctcgca cccgatacat
     2521 cagaagcccg gcacttttct tggccatggt ccgtcgcggc aaagtccaca ggactttgtg
     2581 cttgcgctcg ccgcttcctg ctctaatata ggcgcgtgcg aaacactaag gaagtcgacc
     2641 aaaacatggg ccgccgcggg cgaatgagga gtccagccga ggcggaaaac gcggcgtccc
     2701 aggcggcgcc taatcgacac atgagccgag tagaacatga cgcaaattac agaaaacaaa
     2761 cggtcgggga cagttctact cgacactttt tacaaagggt cgttcttcga tccccaatct
     2821 tctccttggt actttcgggc ttgatcgtcg tcggagcggc atggacggtt tcagctccaa
     2881 gattggccgc cgctcagtcc gccacctcag ccaagctggc tgtcgcgccc gatcttgcta
     2941 aacaattcgc aaaattcaaa tctataagaa ttccatacga ttcaaaggac ctcagcgcgc
     3001 gcgaaaagca aatggtcgaa aagctcgtcg acgccgccgg cctcctcgat tgcatctact
     3061 ggcgccagag cgaccccgac ggactgaagc tctatctctc gctggcaaag agcaagaatc
     3121 cgcaggacca gatgctgcgc gaatatctaa aaatcaatgg cggccgctac gatctcatca
     3181 atgacaataa gcccttcgtt ggcacgcagc cgatgccgcc cggtcgtggc ttctatccga
     3241 cggatctcac tcgcagcgca ttcgacgcat atgtcgcggc gcaccccgcg caaaaagccg
     3301 ctctttacag cggtcagacc atagtcaggc gcaagggctc tgccctcgaa gccgttccct
     3361 accacgtcgc cttccatgaa tttctcgtgc ccatggccgc cgatctccga gcagccgccg
     3421 cccttagcga cgatcctgct ttcgcgaaat tcctccgcct gcgcgccgat gccctgctct
     3481 ctgacgatta ctacccgagc gatgtcgcct ggatggacct ggataatccg aaattcgacg
     3541 tcgtcttcgc tccttacgaa gtttaccttg atggtttgct cggcgtgaaa acgtcgtacg
     3601 gcgcgtccat catgattcgc aacgacgcag aaagcaaaaa actcgcggct tttcagaagt
     3661 acgtccccga tctccaggaa tcgcttccac tcccacacga agatttgcct tccgagcgcg
     3721 gccaacgctc gccaatggaa gtggtggacg ccatttatcg cgccggcgac ctgctccacg
     3781 gttaccaggc ggtggcggat aatctgccga acgatccgcg catccaccag gaaaaaggct
     3841 cgaagaaaat cttctggaag aatttcctcg atgcgcgcgt gaactacatc attctgccgc
     3901 tcgccgaacg cctcctgccc gccaatcaag ccaagatggt caccggcgag ggcacgctcg
     3961 acttcgtaat cctccacgaa atttcacacg gactcggccc cacgtacgtg cacggatcta
     4021 atggcaagca acatatcggc gaagcaatcg gcccgcgata ctccgcactc gaggaatcca
     4081 aagcggacat caccggcctg ctctgtgtga aatggctcgt ggatcacgga gcaataccac
     4141 gcggaaagct aaatgaaatc tacgcctcct ttctgggcga aacttttcgc actattcgtt
     4201 tcggaatagc cgaagcccac ggcgctggcg ccatgatgga gctgagctat ctcacggaac
     4261 agggtgcggt ccgccggaac tcttccaccg gcctgtacga agtagatttc gcgaagatgc
     4321 ccacggcaat cgcatcgctc gccaaggaat tgctcgaaca agaggccacg ggagaccgct
     4381 cgcgcaccga gaagtggttc aaaaagtacg cggttatgcc gccacagctt tccgccgcgc
     4441 tcgcgaaagg ctccgacatt ccggtggacg ttgatccgca atttgatttt cacccgccgt
     4501 tgcggtaaga acgtttctta cccagccgat tcgccccagc ctttcaccaa ccattaacgt
     4561 cccgccacca agaccgggac ccacgccgaa aggagaagtt atggcaaaag taaaaccggt
     4621 tccagaaggc ttccactcag tcacgcccta tctcgcggtt gacgacggag cgcgcgcaat
     4681 tgagttctat cagcaggcat ttggagcgaa ggagagaatg cgtatgcccg ggccggacgg
     4741 gaaaatttcc catgccgaga tcacgatcgg caactcgatc attatgctgg gtcccccaca
     4801 ggcgaatact ggagttcgtt ctcctcaatc gcttggcagc tctacggtca gtatcttttt
     4861 gtatcttgac gatgttgact cgaccttcaa gaaggcattg tccgctggcg ccaaggaggc
     4921 gcagccgctc gctgatcaac tctggggtga ccgctacggc cgggtcaccg accccttcgg
     4981 ccattcttgg tcgctggcca ctcacaaaga ggacgtttca gcggaagaga tgagccggcg
     5041 gatgcaggaa gcagcgaaga cgcaacaccg cgcgcagaca gccggctagt aatgcgagga
     5101 ttcggcgaga gtttgaagat ccgctcgagt ttccgatccg aaaactctcg ccgtgatcta
     5161 ctacacagcg ctcgcgaaaa cgtcgctaca gaatgctttg gattccggcg tcaatggaaa
     5221 cgtgcgtttg tggaaagcca ttgatctggt aatttgcgcc ggaagcgcac tcaaagtgga
     5281 agaaccggct gatgtggtag tcctcatcga atgtactgaa cacatcctcg acttccttgg
     5341 ctgaaagccc caatgcatcc agtggcacag ggtgcccgta gctccctgcc gctcccaaat
     5401 acttctgcac tacctggttc agctcaattt tggcgtttga atccggcgtt tgcatcgcca
     5461 tccactccat tcaaaagcga ttccgcagtt ccagacggcg catggtagca gaatacggcg
     5521 cgattatccc tcgggtaaaa cacctgcaaa aagcgtggga atcaaggcac tgcggtcggc
     5581 ggcttgcgtt tcaggggtaa ccaagcttat actaaatcgg cttccagata ggtcgcacaa
     5641 accagtgggc gcgagcccac ttttttgttg gccgaatgaa actggagcgc agttgtccgc
     5701 accagggata actgctgcga ttagaagaga atggacctag aaaggattcg acaggcagcg
     5761 gagcgggtag cccattctga gggtctggaa gtcttcgaca tcgaatggaa ggtcggaaag
     5821 cagcggctgc tgagggttta tatcgaccgc ctgcccgata cggaacaccc tgacgcggct
     5881 gtaagccaca acgactgcca gcgggtgagc gagcagttga gcgttatttt ggatgtggaa
     5941 gagctgattc cgggaacagg cagttatgtt ttggaggtca gctcgccggg gctggatcga
     6001 aagctgatta agccggggga ctacgaacga tttgccggaa ggctggcgaa aatctggctg
     6061 accgaggcgg ttgagaacca gaagtattgg gaaggccggc tggccggcca tgacaatggc
     6121 attgtaaagc tggcggtgcg cgatcgcgaa ttagccgtgc cgtatagcca gattcggaaa
     6181 gcgaatttgg tcgtggaact gtgatgggcc acacgggcat tctgggagaa agaaagatag
     6241 atggtcagtc tgctatatca gacaatcgag cagatcagcc gggagaagca cattgaaccg
     6301 gagatcatcg tcgcggccat cgaggacgcg atggtcgtgg ccgcgcgcaa gtactaccgc
     6361 actgaggaag agctgcgctc gaaattcaac cccgaaagcg gccagataga cgtcttttca
     6421 gttcacatgg tggtggaaga agtcgcggat ccggttcgag agatttctct cacagacgct
     6481 cgcaagcgaa atcccgaaat cgaggtcggc ggagaaatca cgcagacaaa accgaccgac
     6541 gtgctcggga gaatcgccgc gcagactgcc aagcaggtga tcctgcaaaa ggtgcgcgaa
     6601 gccgagcgtg atacgatcta caacgaattt cacacccggg tgggcgagct catcaatagt
     6661 gtggtgaagc ggatggaagg tccggacatg atcgtggacc tcggccgcac cgaagcgcga
     6721 ttgccaaagc gcgaacagtc gcgccttgag gcctacaatg ttggtgaccg gctccgcgta
     6781 acgattcgag cggtcgagcg cgccgcaaag ggaccgcagg tggttgtttc gcgcgcggac
     6841 cccgcgctgg tgcagcgcct tttcgaaatg gaagtgccgg aaatttatga tggcactgtg
     6901 caaatccgcg cggtggcgcg cgaggctggc gagcgtacga aaatcgccgt tgaaagccgc
     6961 gacaaagatg tagatccggt gggcgcgtgc gtcggaatga agggcatgcg cgtgcaatca
     7021 atcatccggg aacttcgcgg cgaaaagatc gacatcatcc cctacagcga agaaacagtc
     7081 gccttcgcgc aaaaagcgct cagccccgcg aaagtcactc gcgtgcagat cgtggatccc
     7141 gaggaaaagc gcctcgaagt gatcgtcgaa gatacgcaat tgtcgctggc cattggcaag
     7201 aaggggcaga acgtacgcct cgccagcaag ctgatcggct ggaatgtaga tatcaagagt
     7261 gaggaagaaa agcggcgcga aatcgaggcg cagatggctg cactcaccgc tcctggcaca
     7321 gcgcttgccg agctcagcgg cgtcggcccg aaaacaatcg agaagctcga agccgcggga
     7381 atcacttcga tcgaaaaact cgccgacatg actccggagc aattagtaga aattccgggc
     7441 atcggcgaga aaatggtgga aaagatccac caatcggttg cggcgtactt tgaggcgctt
     7501 gaggcgcagg ccaatgctcc gattgaaccc tcgaccgaag acgaggcggc ggccgaagcg
     7561 atgccggaag cgagtgttga aacgccagac acaatcgaga cgtcggatca ggcaggaacg
     7621 actgatttga ccgaggaagc gcaggaaccg gcgtcaaacg cggagaaacc gtctgaggag
     7681 gatgaagaaa ccacagcggc cgataaggaa gcagccgcta aggtagacga aagcgcggaa
     7741 aagcaggaaa ctcccgagga atagcaccgc gccgccgaaa tctgtgccga gaacgacttt
     7801 ggacaacgtg tttagagaat agaggctgat gtcagattcc aaccaaatcc gcattaacga
     7861 actggcgcga gagcttgaga tcaaggccaa ggtcttgatc gactttcttc cccagatcgg
     7921 cgtgacggag aaaaagacgc actcaagttc gctcgatctc gagcacgccg agctggcccg
     7981 aaaacatttt cgcgcgattg cggacgcaga agcagcggcg gaggcggcga aaacagccgc
     8041 aaagtccaag cccgctcggc ccgccgcggc acctgtgaag cccgctggac ctgctcccgc
     8101 gccagctgtg catgctcccc cagcgccacc gtccgcgcct gcaagaacat cgcccgtgac
     8161 tggcaccgct ccgcctgcgg cgactccgtc cgcgccggct cgcccgccca tttcgccaag
     8221 caccacggca gccccagcag ctgctcggcc tgttgcgcct tcggcgtcac caaccgcgcc
     8281 acggactgct gctcctccaa gccgaccgcc atccactgta ggaacggctc caggacaacc
     8341 gtctgcaccc ggcgcgatgc gtccggcagc ggcttcttca actcccgcaa ccggagtttc
     8401 gagtgcgccg aggcctgcag gtccgccacc agcgggacaa cctggacgat tccccgcgcg
     8461 tcctggccaa ggactcggcc aaggtcgccc tggcgctcct caggggatgc gccccgctgc
     8521 cgcaccgggt cagtccaggc cgagcggtcc accgagacct ggcggagcac caccatcgag
     8581 atttccgcaa cggccaggca cgggacgccc cggtcaaggt cccgttggaa gccaacgacc
     8641 cggggcaaca ggtgtttccg gagtgccaaa agcggagccc ggcaagcccc tgtaccagcg
     8701 aaaaccgccc gcttcgcgcg gacgcccgac aatcgaaaag cgctatgccg aaggcgagcg
     8761 taggttgcac cctgttcgaa cgcgcgccgg cgtcggagca ggacgcgcgg ctcacgccga
     8821 acctgtggca cccgtacaac gtgagcctcg gaacgtcacg gttacggaag gcatcaccgt
     8881 ccgcgaactt gccgaaaaga tggatattcg cgctaaggac ttgctgaaaa ccttgctaga
     8941 ccgtggcgtt ttcgccagca tcaaccaagc gctcgatgtc cccactgcca ctagcctagc
     9001 ggaagcgttt aacggaatcg tccaagtggt cacgtttgaa gagcaggtgg tccaggaaga
     9061 gaaaatcaag gaagagcgaa ccgggaacca gctccctcgc gcgcccgtgg tcaccgtgat
     9121 gggccacgtg gaccacggca aaaccagctt gcttgatgcc atccgcgaaa ctgaagttgc
     9181 ggcaggcgaa gcgggaggaa ttacccagca tatcggcgcg tatgaagcgc acgtggatca
     9241 gcgtcgaatt gtttttatcg acacgccggg ccacgaagcg ttcacacgaa tgcgcgcgcg
     9301 cggcgcaaag gttaccgata ttgtcgtcct cgtagtagcg gcggacgatg gcgtgatgcc
     9361 gcagacggaa gaagctattg cgcacgccaa agccgccaac gtgcccatcg ttgtcgccgt
     9421 gaataaaatc gacaagccgg aggctctgcc tgagcgcgtg aaacgccaac ttgccgaccg
     9481 cggattgatg cccgaagagt ggggcggcga tacggtgatg gtggaagttt ccgcaagggc
     9541 caagaaaaat ttgccacgcc tgctcgagat gattctgctc gtcagcgatt tgcgcgaact
     9601 caaggccgat cccgacgctc ctggcagcgg tacggttctt gaatcccgca tggacaaggg
     9661 ccgcggacct gtggctaccg tgctagtgca aaacggaacc ctgcgcgtcg gcgacgtctt
     9721 catcgttggc gctgtttatg gcaaagttcg cgccatgttt gacgatcgcg gccaagcaat
     9781 ccagaaggcc gagccgtcaa cgcctgttga agttctcgga ttgcaaggcg tgcccgaagc
     9841 gggcgaccaa ttccaagtgg cggacgaagc gaaggctcgc cacatcgtcg agtaccgtca
     9901 aggcaagcag cgcgaggctg ctctggcgaa gaccggccct tccgggcgac tcacgctcga
     9961 tcaattgcac gagcaactcc gcgcgggcga gatgaaagag ctgcccgtgg tggtgaaagc
    10021 ggacgtgcaa ggttcggtgg aagtcctcaa tgaattgctg ccggggcttt cgaacgatca
    10081 ggtgcggctg aaagtgattc aggcgagcgt gggcgcggtc accgaacgcg acgtactgct
    10141 cgcctcagct tcgaatgcga ttattgtcgc attcaacgtg aggcctgagc gcaaagcatc
    10201 tgatctggcg cagcgcgaag gtgtggacat tcgcctccac acgattatct acgagctgat
    10261 agacgagctt aagaaagctg tctccgggct gctggcgccg ctcatcaagg aaacctatct
    10321 cggccgcgct gaagttcgcg acacattccg cgtaaagggc gtgggtatga tcgcaggatg
    10381 ctccgtacag gaaggcatcc tgaagcggga tgcggacgtt cgcgtgctcc gcgacaacgt
    10441 ggttatctac acgggcagaa tcagttccct gcggcgattc aaggaagatg ccaacgaagt
    10501 gcgcgccggt ttcgagtgcg gcgcgggcat ctctaatttc aacgatgtga aagttggcga
    10561 ttccctggaa tgcttcaaga tcgagaaggt ggcgccggtc gaagctccgc ccgatcgcgc
    10621 ggcacggccg caagcggtcg tggaagccag gcgcggctag aaagccgcac gcgctggcgt
    10681 atcgccggaa tttctcggtg cggatcgaag cttggactct cggctcgctt cactgttgcc
    10741 cccgaaagtg gggctccttc gttacctcgg gatgaacgga aatgccggtc ggcctcctca
    10801 cactggaaat tcacatttct gacgcgcagt cgttgaaaga taagcggcag gtgcttcgca
    10861 gcctgaaaga tcgattgcgc gcccatttca atgtggccgt ctccgagttg gattatcagg
    10921 aagtttggca acggtcgcgc gtgggagttg tcaccatttc gggtgacggc aagcatctcg
    10981 aggaatcggt tcaagcaatc gcggccgaat ccgagcgcat cctggggcgc gatctgcttt
    11041 ccagccaaat tgaatacttc gaggatggag aatgacggtc ggtggattcc gcgctgaaag
    11101 aattgcggaa gaaatccgca acgaagtcgg cctaatgctg gccggcgagc tgaaagatcc
    11161 gcgccttgca gttcccctca gcgtcacgga ggttcgcgtc gcgcccggca tgcgcgtcgt
    11221 gcgcgtattc gtggaactcc aggggaatga cgcggagcga agcaaggctc tcgcgggatt
    11281 gaaagctgcc gccggctttg tccggcacga attggtcgag cgcctgcggc tgcgaagagc
    11341 acccgaaata attttcctcg ccgatgagtc cgaagaatac ggccagcaca tcgacgaact
    11401 actgcgaaaa gtgcatcacc cggagaaatc gtagcgggcg tcgcccgaaa cttgccggct
    11461 cgaatcggac gctacgaacg aaaccaaaat gtcgcgaatc ccgcaaccgc ctccgctcga
    11521 tggaatcctc gtggtcgata agccgcgggg aaagacgtct catgacgtcg tcgaaaccgt
    11581 gcgccgcctc gccggcttcc ggcaaatcgg ccatcttggc acgctcgacc cgctggctac
    11641 gggtgtgctg gtgctcgcgc ttggcaaagc gacgcggctt gcgcgcttct ataagggacg
    11701 ccggaaacgc tacacctttg cggtgcgttt cggattcgct acggatactt atgacgccga
    11761 tggcgaagct ctgggcgcgg atgcggaacc cgcgctagat gaggcggaaa ttcaggcgct
    11821 cgccgcgcaa ttcacaggaa aactccagca aaccccgccg gccttttcgg ccaaaaagat
    11881 cggcggccgg ccggcgcacg aactcgcgcg caaaaataaa cccgtgaagc tggaaccggt
    11941 tgatgtggag gtatacgaat tcaggctaac cggaatcgac ggcgctatcg ctcgcttttc
    12001 ggtcgaatgt ggcgcgggca cttacattcg ttcgctggcc cacgacctgg gcaagcttca
    12061 cggttcgggc gctcatcttg cggaaataac ccgcacggca gtgggggaat ttacactcga
    12121 tcacgccgcg ccgcttgctg aactcgtcga ggacgcgaga tcgggaaaga tcgccgcgcg
    12181 cgtgatccgg ctcgagggac tgctttccga attgccgcgc gccaccgtgc tgcccatcgt
    12241 cgagaaacgc gtgcgccacg gcgccaagtt caatttgccg ctcgcacaaa ttcagccggg
    12301 ccatgcaacc gccgcgcaag gagcgcccgc cgagctcgat tcaggtgaat ggcagccttc
    12361 gcgattgcgg gtcttcagtc agcaggggca attgatcgcc atcgccgaac ctgtcgtccc
    12421 gcgcacgtat cagcccgtag tcgtgctcga cgccgcgcaa taatccgcgc aataatcatt
    12481 gctcgttggc gcgatcacga cgccgagccg aatctctatt gatccggcgt aggctgggtg
    12541 ccgcgctgtg tcggaagagg cgggggactc ggcggcggcg gctcctcatt ctgctccgga
    12601 acttgctgcg gtggaggctg cggcgcgaat gtgttttcca ccgcgccgcc cccggcgcct
    12661 tccgaggcac cctgccccgc gtaaatcgta tggtccttgc gccgggcgag tcgcaccatc
    12721 cgcggcgtga ccaggatcag caactcggtt tcctgctgct gcgcgtttcg attctgatcc
    12781 agaagcccga ctcccggaat ttcggcgagg cctggattgc cggtgatcgc gtttgtgagc
    12841 tgcgtttcca ggaaccctgc cagcgccgcc gtttcattgg gcttcaaccg caccgtctga
    12901 tccacttcgt cattgctgat gaccgggatc gcattgatgc tctggctgct caggctgcta
    12961 atatcgaaat tcagctcgag agtaacgtca ttgtcaggat gaattcgggg cgtggccttc
    13021 accttcaggc cgatatcgag atactgcact cctggataag gcgttccgaa ggaattgagg
    13081 cccgcgctgc cgaaaaggct agcggaattg attattaccg tggcctgggc ggacgcgcta
    13141 tctgccgtca cggaatcagg caggccatcg gcattgaaat cggccgtcgc gatggaagag
    13201 ggagccgtgc ccaccggtat ttccggaagt gccgtgaaaa gcccatcgcc gccgtttaga
    13261 agggcggtta cggcattgtc cgctgtgtcg gagacagcga tatcggcgag accatccgta
    13321 ttgtaatccg ccacggcgat agcagtcgga ccgttgcctg cagcgaagtc ccgcatagcg
    13381 cctgcggcga acgtgcccag gggcgaggag gtggttgagc tgatttgatt gtaataaacc
    13441 gttaccgagt taccgctgag gttcgctgcc gcgatgtcct cggcgccatc gccattaaag
    13501 tctccaaacg ctactgatac gggaccggct ccagtcgggt agtcggtgcg attcctaaat
    13561 gttccgtcgc cattaccaag gaaaacggaa attgtgttgt ggcctcggtc cgcgacaaca
    13621 atatccgcgt ggccgtcgtc gttgaggtcc gtggccgcaa gagcggcagg tgaaaaaccg
    13681 gcgggaagtt ggatcaacgc tggcgcctga aacgtgccgt caccattgcc ctggaagatt
    13741 gaaattgaat tatcatcctg gttggcgacc gccagatcga ggaacccagt gccggtgccg
    13801 tcgtggaaat tcgcggtagt cacggagaca ggggccctgc ccgtagggat gtccgctttg
    13861 ggctggaatg tgccgtcgcc tttccccagc aaaatcgaaa tcgtatttgc ggccttattg
    13921 gcaaccgcca gatccaaaat gttatcgcta tttgcagcgg tcgtgccgtt attgaactgc
    13981 gcgctcgtga tccaaaccgg attcgtcccc gtcggaaaag tcgcctgcgc gccaaatgtg
    14041 ccgtcgccat ttccaagcaa gacggacacc gtattgtcgg ccgaattcgc gacgattagg
    14101 tcgttgatgc tattggcgcg aagatcggcc gtggtcacga aaga
//
LOCUS       DATNDT010000338        10926 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2395880, whole genome shotgun sequence.
ACCESSION   DATNDT010000338 DATNDT010000000
VERSION     DATNDT010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10926
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2395880"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(218..1402)
                     /gene="hpnI"
                     /locus_tag="VN788_10915"
     CDS             complement(218..1402)
                     /gene="hpnI"
                     /locus_tag="VN788_10915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriohopanetetrol glucosamine biosynthesis
                     glycosyltransferase HpnI"
                     /protein_id="HXU21082.1"
                     /translation="MTISWIAVRDVFLLLSLAPFAYYLLAIFAAQRFFRGVGPSLAVP
                     LPNHPPGISILKPIHGLDRETYQNYASFCRQNYPEFEILFCVSDAGDPAVPVIANLIR
                     DFPERSIRLLIGSEPLGASDKVNKLARMAREATHEVLIVSDSDVRVGPNFLRAIATSL
                     ADPKVGGVTCLYRGLVEECFAATLEALGNSTDFAPGVLVAWLLGNHALDFMLGAVMAT
                     TKTHLAEIGGFESLVDYFSDDYELGNRLAASGHRVKLSRFPVSIVYQSERLPAAFRHQ
                     LRWNLSIRYSRPWGHLGLIFTQGLPWTVLALILAPSPDVAAEYIAAYVLLRGTMAWVV
                     GAWGMRDRLVRHKFWALPLRDAFAFFVWLASFFPQRIHWRGQQFLVRKKHLVAVSSPP
                     RP"
     gene            complement(1418..1795)
                     /locus_tag="VN788_10920"
     CDS             complement(1418..1795)
                     /locus_tag="VN788_10920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXU21083.1"
                     /translation="MVTQAILYILIVFVGTGGELCVSRAMKTIGEVTDFRPHAIARVV
                     ARSLALPRMWMGIALMSIAFFALLGMLSLAKVSFVFPSTALSYAAGALGGRYFLNERV
                     TPQRWFGVLLVCVGVALVFLGKG"
     gene            complement(1789..2211)
                     /locus_tag="VN788_10925"
     CDS             complement(1789..2211)
                     /locus_tag="VN788_10925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HXU21084.1"
                     /translation="MTSAQGLHLKTIALIVIMISVAPLGDVFLGKGMKHLPPMASWAP
                     GHLFHFFFGAFTTSAVWIGIGLLLAYFIAYLVVLSWADYSYVQPASSASYVLIALLAH
                     FVLRETISPMRWLGVAVICTGVFVVGHTHPQTPEHHSW"
     gene            complement(2208..3716)
                     /gene="hpnJ"
                     /locus_tag="VN788_10930"
     CDS             complement(2208..3716)
                     /gene="hpnJ"
                     /locus_tag="VN788_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis associated radical SAM
                     protein HpnJ"
                     /protein_id="HXU21085.1"
                     /translation="MKTLFLNPPSFEKFDGGAGSRWPATREIESYWYPVWLAYPAGML
                     PGSRLLDGPPHHITPTEVVNTSKDYDFVVLFTSTVGFNSDLRMARAMKEQKPDIKIAF
                     VGPHVQIKPAESLLASSDLDFVVRGEFDHAVVEFAQGKPISEIGNASYIKDGKVIHNP
                     SRPPLQTEELDALPFATDVYQNNLTIENYNVPFLLHPFVSFYTARGCPALCTFCLWPQ
                     TLSGHAWRTRSVESVAREFKQAHKMFPQAKEFFFDDDTFNIRKDRVVALCKEFEPIGF
                     QWSCTARVHGDYDTLKAMANAGCRLLIVGFESGDAQILKNIKKGQTLEMGRNFVKNCK
                     KVGIRVHGDFIIGLPGETKETIEKTINFAKDLDCETIQVSMAHAYPGTELYDQGTREG
                     FLAGEAITDIGGHQLPHLEYPGLSKEYMVECVNRFYDSYYFRPKVAWRIVRDALWDGH
                     ERKRLYHEAVDFMKLRADRIKVSRARKGVDKKALVTVPPMDASSGVGESQGA"
     gene            complement(3828..5153)
                     /locus_tag="VN788_10935"
     CDS             complement(3828..5153)
                     /locus_tag="VN788_10935"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXU21086.1"
                     /translation="MSGLRNCTLALAAGIALLFARPARAQGVSTLTLREAVTLALQNS
                     RDMRLAQVQYNVALGEVRVNRAAFLPNLYTGSGAAYTHGFPSLPGGHAPAVFELDYTQ
                     SVFDPVLKAQQHAAEERAKNQKIELDRTRDDVVVRTATAYLELAKVRHSLDLMRSEQA
                     SGEKILQVVRERVAANQELPMEGTRSQLAVARIQERIVKLEDREGILDQQIRDLTGIP
                     DSQSLQVEAEDPAFTTELATDVAAVQSESQMVSLAVQNDRDVAEAEHERKAREDISRG
                     ARWSYWPTVAVVGEYSILSRFNNYDQFYKTFERNNVNVGVQITIPIFAAKTSANVALA
                     KSQLAEAQLNLANKRQEVRLNVQQKARSVRELDAAREVARLDLQLSQETVQVQQAKFD
                     QGRITLQEIEQARLDENDKWVAFLDADFARQQAQLTLLQATGQLAKVLP"
     gene            complement(5179..6423)
                     /locus_tag="VN788_10940"
     CDS             complement(5179..6423)
                     /locus_tag="VN788_10940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU21087.1"
                     /translation="MANARDTSAFWGETWKLAMQALRANRMRAALTMLGVVIGSASIV
                     LVVTIALTGKQYALAQIEGVGANLVYTNVVNSGDPGSLNLADQITPADLAAVKEGLSQ
                     LVTDVAGTSAQQLTVSINGQERPINLVGVTRDYEKIHNLAIVRGRYFDESDLTARSKV
                     CLITEDLARRVFPFENPDGKQISAGELHFTVIGVFRERLAILGPTEITRDSAIIPFSL
                     LRYYTGADYFATLYAKARRSDDVPAVTQQVKEIIQSRHRPGAQYRVQNLSGILETAEA
                     IALALTVVLVLIALIALAIGGVGIMNIMLVTVTERTREIGIRKAIGASGDAIRYQFLM
                     EAMAISGTGALAGILIAVLIPTLLQLLLRFFPDTQNINVPISWLSVVLAFVVSCSTGL
                     IFGYLPANRAARLQPTEALRHE"
     gene            complement(6504..7508)
                     /locus_tag="VN788_10945"
     CDS             complement(6504..7508)
                     /locus_tag="VN788_10945"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU21088.1"
                     /translation="MKAPNWVASLILSVVLFLLSGFSAPVQADAGSAVEAARQQFNAG
                     NYAAAISTLQATAPQSTSSAAMAYWLGRSYYEILDYDSAIAQLERAVSLDPKNSLYHQ
                     WLGRADGGKADRDSSFSMAKKVKKEFQQAVVLDPSNVAARRDLEEYCLDAPWIAGGSK
                     SEAVDQVNAIAALDPIQGHLARALYYRKGLKKLDDAENEIRQVLSAKPNRADPYFEAA
                     DFFEARNQATDVQTAVQSAAQVAPNDPRLAFYRGVAGVLANANLVVADEDLKAYLAST
                     PERSDWPSHASARKWLGRLYEVQGKPAEAAEQYRAALRLEPKNKDARVRLERLEKSSP
                     "
     gene            complement(7505..8743)
                     /locus_tag="VN788_10950"
     CDS             complement(7505..8743)
                     /locus_tag="VN788_10950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HXU21089.1"
                     /translation="MAQSKKRNKTIIAVLIACLVVLIVIVLGLRGQPPLVPVVTVTRQ
                     DLNVDITSNGKVEPISPAVARAEFPTFVKNVRAVEGQAVHRGEVILTLDAADIRSQLA
                     EARANSLAAQTDLRNARSGGPPDQVAQLQGDLQTARVQLKNLETAEKALRELIAKQAA
                     TQDELAQNEASLAKARANLSALEARKQDLQQRSAASIEVARLRYSKAQDEVRSLEEKA
                     RSATVISPFDGTLYSLPVHTGDYVRVGDTLAEMADLRHIRVRAFVDEPDLGLLEPSQD
                     VEVTWDAKPGSVWNGHTEQVPKQVVSRGMRSVGEVLCSIDNDKLELLPNTNVQVKILV
                     HERRGAVVVPRAAVLEDKGGHYVFVFNGNKVRRRNISVGIASTSKYEVMSGLMVGDRV
                     AEPVDQNLKNGMEVRPGEAY"
     gene            8986..9441
                     /locus_tag="VN788_10955"
     CDS             8986..9441
                     /locus_tag="VN788_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXU21090.1"
                     /translation="MSYIERIQNDLTAAMKGKDELRLSVLRMVKSALKNKEIEKMRPL
                     EDMESLQILQTLVKQRRESIDQFRQGGRKDLAEKEAKEIAIIEEYLPAAPSDEEVRRA
                     VEEAISETSAGSVRQMGAVIKAARAKLEGKAVDGKVLSDHVRERLSEKS"
     gene            complement(9586..>10926)
                     /locus_tag="VN788_10960"
     CDS             complement(9586..>10926)
                     /locus_tag="VN788_10960"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HXU21091.1"
                     /translation="HTGDIGERDEAGNLYFKGRRKNVIVTPEGLNIFPQDLEVELRKE
                     PSVRDCVVIALERDGNAQPCAVLILKDAANTSEAAATVQRANERLAPFQRMQHWFVWP
                     DADFPRTPTQKPALGRIREAAEAALGGRRSGSSQAAPSGSIGELLKGISRGRLPSNES
                     ASLQLGSIERVELLSTLEDRYQVDLSETEFASANTIEALEKLIAQPSRGAVTVHYPRW
                     PQSWPARLLRAAVFAVLVRPALLILGWPRVSGRENLRNVKAPTLVISNHVTYFDPVLI
                     LHALPARLRHRLAVAMDGERLESMRNPSPDLEFFSGLLNRTQYFLALALFNVFPLPRQ
                     AGFRKSFAFAGDLIDRGWSVLIFPEGELTRDGRIAPFRAGVGLLAAGLGAPVVPARLD
                     GLFALKQAGKRRAAPGQIKITLGAPISIDAGRPAEAITRDLQQHVAGLAENGGK"
BASE COUNT         2369 a         3040 c         3281 g         2236 t
ORIGIN      
        1 gggaggcaag tcgtgattac gtagtccgag gatacaaaca aaattctaca gaacgtggcg
       61 taagacccgc aagtggttgt tggtgtaaag aaaaatcgtc cacggtgcgt gtggactttc
      121 ggtgtttgta tgggcacggc gcagttgcag aatgactggc ggtcgcgtcc gtatgccgag
      181 acggatggtg aacgcgacgc caaggtgggt tgagagacta tggccgcgga ggcgatgaga
      241 ctgcgacgag gtgttttttg cgaacgagga attgctggcc gcgccagtga atgcgctgcg
      301 ggaaaaagct ggcgagccag acgaagaatg cgaaggcgtc gcgcagagga agcgcccaga
      361 atttatggcg caccaggcga tcgcgcattc cccaggcgcc tacgacccaa gccatcgttc
      421 cgcgcaggag tacgtaggca gcgatatatt ccgcggcaac gtccggcgac ggcgccagga
      481 tgagcgcgag cacggtccac ggcaaacctt gggtgaaaat aaggccaaga tgcccccagg
      541 gtcgcgaata gcgaatgcta aggttccagc gaagctgatg gcgaaaagcg gcgggcagtc
      601 tttcagactg gtaaacgatg gaaacgggga atctgctgag tttaacgcga tggccgctag
      661 cggcaaggcg attgccgagt tcgtagtcgt cggagaaata atcgaccaag gactcgaaac
      721 cgccgatttc ggcgaggtgc gttttggtgg tcgccatcac agcgccaagc atgaaatcga
      781 gcgcatgatt tccgaggagc caggcgacga ggacgccggg cgcgaaatcc gtgctattgc
      841 cgagggcttc cagagtggcg gcgaaacatt cttcgaccaa gccgcgatag aggcaggtga
      901 ctcccccgac cttgggatcg gcaagagatg tggcgatggc ccgcagaaaa tttggtccga
      961 cgcggacgtc actgtcactg acgatcagga cttcgtgcgt ggcctcgcgg gccattcgcg
     1021 cgagcttgtt gactttatcg ctcgccccca aaggctcgga accgatgaga aggcggatgg
     1081 agcgctcagg gaaatcgcgg atcagatttg ctatcactgg gacggcagga tcgccggcat
     1141 ccgacacaca gaagaggatc tcgaattcgg gataattctg gcgacaaaaa ctggcgtagt
     1201 tttggtatgt ttcgcgatcc agcccatgga tgggtttcag aatgctgatg ccgggagggt
     1261 ggttggggag aggaacggcg agagaggggc ccactccgcg aaaaaaacgc tgcgcagcga
     1321 aaatggcgag cagatagtag gcgaaaggcg ccagagaaag aagaaggaac acatcgcgaa
     1381 ccgctatcca ggagatcgtc acgcaatcct cgctctgcta tcctttgccg agaaacacga
     1441 gtgcgacgcc aacgcagacc aacaaaacgc cgaaccagcg ctgcggcgtc actcgttcat
     1501 tgagaaaata cctgccacca agcgcgcccg ccgcgtagct gagggccgtt gacgggaaca
     1561 cgaagctgac tttggcgagc gacagcatgc cgagcagcgc gaaaaacgcg atcgacatca
     1621 gggcaattcc catccacata cgcgggagcg ccaaggagcg tgcgacaacg cgagcgatgg
     1681 cgtgcggacg gaaatcggtg acctcgccga tggttttcat cgcgcgggaa acgcaaagct
     1741 cgccgccggt gccgacgaaa acgatgagga tatagagaat agcctgggtt accacgaatg
     1801 gtgctccggc gtttggggat gtgtgtggcc gacgacgaaa actccggtgc agatgacggc
     1861 aactccaagc cagcgcatcg gcgaaatagt ctcgcgcagc acgaagtgcg ccagcagggc
     1921 gatcaagacg taggacgcgg acgaagcggg ctggacatag ctatagtccg cccaggagag
     1981 gactacaagg taggcaatga aataggcgag gagaagtccg attccaatcc agacggcact
     2041 agtggtgaaa gcaccgaaga agaaatgaaa cagatggccc ggcgcccaac tggccattgg
     2101 gggaagatgc ttcattccct tgccaagaaa cacgtcgccg aggggggcga cggaaatcat
     2161 gattacgatc agagcgattg tcttgaggtg gaggccctgc gccgacgtca tgcgccttgc
     2221 gactcaccga ccccggaact cgcgtccatc ggcggaaccg tgacgagcgc ctttttgtcg
     2281 acacccttgc gggcgcgcga aactttgatg cggtccgctc ggagcttcat gaaatctacg
     2341 gcctcgtgat aaaggcgctt gcgctcgtgc ccatcccaca gcgcgtcgcg aacaatgcgc
     2401 caggcgacct tgggccggaa atagtagctg tcataaaagc ggttaacgca ctcgaccatg
     2461 tattccttcg acaggccggg atattcgagg tgagggagct ggtggccgcc gatgtcggtg
     2521 atggcttcgc ccgccaagaa gccttcgcga gttccctgat catacaactc ggtgccggga
     2581 taggcgtggg ccatggagac ctggatcgtt tcgcaatcga gatctttcgc gaaattgatg
     2641 gttttctcga tggtttcttt cgtttcgccg ggtaggccga tgatgaaatc gccgtggacg
     2701 cgaatgccga cctttttgca gttcttcacg aagttgcgtc ccatctcgag cgtctggccc
     2761 ttcttgatat ttttcagaat ttgagcatcg cccgattcga agcccacgat cagcagacga
     2821 caaccggcgt tggccatggc tttcagagtg tcgtagtcgc cgtggacgcg cgccgtgcag
     2881 gaccattgaa agccgatcgg ctcgaattcc ttacaaaggg ctacaacgcg atctttgcgg
     2941 atattaaagg tatcgtcgtc aaagaagaat tccttggcct gagggaacat tttgtgagct
     3001 tgcttgaatt cgcgtgcaac gctctcgacg gatctcgtgc gccaggcgtg gcccgaaagc
     3061 gtttgcggcc acaggcagaa cgtacacaac gccggacatc cgcgcgccgt gtagaaggaa
     3121 acgaaaggat ggagcaggaa cggcacgttg taattctcaa tcgttaaatt attctgatag
     3181 acatcggtcg caaatggcag agcgtcaagc tcttcggttt gcagcggcgg gcgcgacgga
     3241 ttgtgaatta ctttgccgtc ttttatgtag ctggcattgc caatttccga gatgggcttg
     3301 ccttgcgcaa attccaccac ggcatgatca aattcgccgc ggaccacaaa atcgagatcg
     3361 gaactggcca gcaagctttc agccggctta atctgcacgt gcggaccgac aaaagcgatc
     3421 ttgatatcag gcttctgctc tttcatggcg cgggccatgc gcagatcgct gttaaaacct
     3481 acggtggaag tgaacaacac gacgaagtcg taatccttcg aggtattcac cacttcagtg
     3541 ggcgtaatgt ggtggggcgg gccgtcgagc agacggctgc cgggcagcat gccagccggg
     3601 taagcgagcc agacggggta ccagtaggac tctatctcgc gcgtggcggg ccagcgagaa
     3661 cccgcaccgc catcgaattt ctcgaagctc ggcggattca gaaaaagtgt cttcattgca
     3721 gacgacatct ttacaccatc ctcagagctg agatgcagcc accgtaagaa actcacccaa
     3781 ttctactcga tttcaatagc atgcccaata tttctgtgaa tttgttgcta cggaaggact
     3841 tttgcaagtt ggccggtagc ctgcagcagc gtgagctggg cctgctggcg agcgaaatcg
     3901 gcatccagaa aggcaaccca tttgtcgttt tcatccaacc gagcctgctc gatttcctgg
     3961 agagttatgc ggccctgatc aaacttggct tgctggacct gcacggtttc ttgcgacaat
     4021 tgaagatcca ggcgggctac ttcgcgtgct gcgtccagtt cgcgcacgct gcgggctttc
     4081 tgctgaacgt ttagacgaac ttcctggcgc ttgttggcaa ggttgagctg agcctcggca
     4141 agttgggatt tcgccaacgc aacattggcg gacgtcttgg ccgcgaagat cggaatagtg
     4201 atctgcacgc cgacgttgac attattgcgc tcgaacgttt tgtagaactg atcgtagtta
     4261 ttgaacctgc tcagaataga gtattccccg acgaccgcga cggtgggcca gtagctccag
     4321 cgcgccccgc gggaaatgtc ttcccgggcc ttgcgctcgt gctccgcttc ggcgacgtcg
     4381 cgatcatttt gtacggcgag gctgaccatt tgcgactcgg actggacggc tgcgacgtcg
     4441 gtggcaagct cagtggtaaa tgcagggtcc tcggcctcga cctgaagaga ctggctatcc
     4501 gggatgcccg taagatcacg gatctgctgg tccaatatcc cttctctgtc ttcgagcttc
     4561 acgatacgtt cctggatgcg agccacggcc aactggcttc gcgttccctc cattggcaac
     4621 tcctgattcg cggcgactcg ttcgcggacg acttgaagga ttttttcgcc gctggcttgt
     4681 tcggaacgca tcagatcaag ggaatgccgt actttcgcta gctcgaggta agcggtagcg
     4741 gtgcggacca cgacgtcgtc gcgggtgcgg tcaagttcga tcttctgatt tttggctctt
     4801 tcctctgccg catgctgctg agccttgagg acgggatcga acacgctttg cgtgtaatcc
     4861 agttcaaata cggcaggggc gtgccctcca ggcaatgatg gaaagccgtg tgtgtatgcc
     4921 gcgccggaac ctgtgtacaa gttgggcaag aacgcggccc ggtttacgcg cacttcgccg
     4981 agagcgacgt tgtactgcac ctgcgcgagc ctcatgtcgc ggctattttg gagcgcgagc
     5041 gtcacggcct cacggagggt cagtgttgaa acgccctgcg cgcgcgcggg ccttgcgaac
     5101 aacagcgcga tgccggcggc cagcgcgagc gtgcaatttc tgagaccact cattcgactg
     5161 ctaccgcgat tcgcaacgct actcatggcg caacgcctcc gtcggctgaa gtctcgctgc
     5221 tcggttggcc gggagataac caaagatcaa accggtagag caagatacta cgaacgccag
     5281 aaccactgag agccacgata tgggcacgtt gatattctgg gtatctggaa agaaccgcag
     5341 caacaactga agcagggtag gaatcagaac ggcaatcaat attccggcaa gcgctcctgt
     5401 cccactgata gccatagctt ccatcaggaa ctggtagcga atggcgtcac ccgaggctcc
     5461 gatggcttta cgaatcccga tctcacgggt ccgctcggtg accgtaacaa gcatgatgtt
     5521 catgattcca actccgccga tggccagagc gatcagggcg atcaaaacga gaaccacggt
     5581 gagcgcgagc gcgattgctt ccgccgtttc gagaatgccg ctgagatttt gcacgcgata
     5641 ttgggcaccg ggacggtggc ggctttggat aatctccttc acctgctgcg tgacggcagg
     5701 gacatcatcg gaacgccgcg ccttggcgta tagcgtggcg aaatagtcgg cgccggtgta
     5761 ataacgcagc aacgagaagg ggataatggc cgagtctctg gtaatttcgg tggggcccag
     5821 gatggcgagg cgctctcgaa acactccgat cacagtgaaa tggagttccc cggcgctaat
     5881 ctgctttcca tcgggattct caaatggaaa aacgcgccgg gcgagatctt ccgtaatcaa
     5941 gcagactttg ctgcgcgccg tcaaatcgga ttcatcgaaa taacgtccgc gaacgattgc
     6001 caggttgtgg atcttttcat agtctctggt gacgcccacc agattgatgg gccgctcctg
     6061 gccattgatg gatacggtca gttgctgggc actggttccg gcgacatccg tgacaagttg
     6121 cgacagacct tccttcacgg ccgctaaatc cgcgggggtg atttggtcgg ccagattcaa
     6181 gcttccggga tctcccgagt tgaccacatt ggtgtacacc aggtttgcgc ccacgccctc
     6241 gatctgcgcc agcgcgtatt gctttccggt aagtgcgatc gttaccacaa gaacgatcga
     6301 cgcgcttccg ataacgacgc cgagcatggt gagggcggcg cgcattctgt tggcgcgcag
     6361 agcctgcatg gcgagcttcc acgtctcacc ccagaatgcc gatgtgtcgc gcgcgttggc
     6421 cattcgaatt aacgcgaagg tctatctaag gcacaaggac cccgggctgc agccaaagac
     6481 gaatccacca aaaaagggcc gttttatggc gaagactttt ctagccgctc gagccgcact
     6541 cgcgcgtcct tgtttttggg ctccaagcgt agcgccgcgc gatactgttc cgctgcctcg
     6601 gcgggtttgc cctggacttc atagagcctg cccagccatt tgcgtgcgct tgcatgggaa
     6661 ggccagtcac tgcgttccgg ggtgcttgcg agataggcct ttagatcctc gtccgcaacc
     6721 accaagttcg cattggccag cacgccggcg acgccacgat agaaggcaag ccgcggatcg
     6781 ttgggtgcca cctgcgccgc ggattggacg gcagtttgga catcggttgc ttggttccga
     6841 gcctcgaaga aatcggcggc ctcaaagtag gggtcggccc tatttggctt ggcactcagg
     6901 acctggcgaa tttcattctc agcatcatcc agcttcttga gacctttgcg gtaataaaga
     6961 gcacgggcca agtggccttg gattgggtcg agtgctgcaa tggcgttcac ctgatcgact
     7021 gcctcgcttt tgctgccccc ggcgatccag ggggcgtcaa ggcagtattc ttcgaggtcg
     7081 cgccgggcgg caacattgga agggtcgagc accacagcct gctgaaactc ctttttcacc
     7141 tttttggcca tcgaaaagct gctgtcacga tccgccttgc cgccatcggc acggcccagc
     7201 cattggtgat aaagtgaatt cttcgggtcg agagacaccg ctctttccaa ctgggcgatt
     7261 gcactgtcgt aatcgaggat ttcgtagtaa ctgcgcccca gccagtatgc catggcggcg
     7321 ctcgaagtac tctgcggtgc cgtcgcttgc agcgtggaaa tcgctgctgc gtagttgccg
     7381 gcattaaact gctgccgggc tgcctcaacc gcactgcccg catcggcttg caccggcgcc
     7441 gaaaaaccgc ttagcaaaaa gagaacaacg gacaaaatca ggctggctac ccagtttggc
     7501 gctttcaata ggcttcccct ggccgtactt ccatgccgtt cttcaggttc tggtcgaccg
     7561 gttccgctac ccggtcgccc accatcaatc cggacatcac ctcgtatttc gaggtgctgg
     7621 ctatacctac agagatgttg cgccggcgca ctttgttgcc attgaaaacg aagacatagt
     7681 gcccgccctt atcttccagt acggcggcgc gcggcaccac gaccgcaccg cgccgttcgt
     7741 gcaccaggat ctttacttgc acgttggtgt tcggcaataa ctccagcttg tcattgtcga
     7801 tcgagcaaag gacttcgccc acactgcgca tgccccgcga aaccacttgt tttggtacct
     7861 gctcagtgtg gccattccaa acgctgccag gcttggcatc ccaggtgact tcgacgtctt
     7921 ggctcggctc gagaagccct aagtcgggtt cgtcgacgaa ggcccgcaca cgaatgtgcc
     7981 gcaggtccgc catttcggcc aatgtatcgc ccaccctgac ataatcaccc gtgtgtacag
     8041 gcaacgagta gagtgtgcca tcgaacggcg agattacagt ggccgagcgc gccttctcct
     8101 cgagggaccg gacctcgtcc tgagccttgc tgtaccggag cctcgccacc tcgatgcttg
     8161 ccgccgacct ttgttgcaag tcctgctttc gagcctcaag cgcgctcaga ttggcgcgcg
     8221 ccttggccaa actagcctcg ttctgagcga gttcgtcttg cgtagccgcc tgtttggcga
     8281 tgagttcgcg cagggctttt tcggcagttt caagattctt cagttgcaca cgtgcggtct
     8341 gaagatcgcc ttgaagctgc gcgacttggt ccgggggacc tccactccgc gcgttgcgaa
     8401 gatctgtttg cgcggcgagg ctattggcgc gcgcctctgc cagttgggag cgaatatcag
     8461 cggcatcgag cgtcaaaatc acctcgcctc gatggacggc ctgcccttcg acagccctca
     8521 cgttcttaac aaacgtaggg aattccgcgc gcgccacggc gggcgaaata ggctccactt
     8581 tcccattact cgtgatatcc acattcaaat cttggcgcgt taccgttaca accggaacca
     8641 gcggaggctg gccgcgtagc ccgaggacga ttacaatgag cacgaccagg cacgcaatca
     8701 gaaccgcgat aattgttttg ttccgctttt tgctttgcgc catttaatcg accgcatacg
     8761 cgatcagccc aagtgccgaa tcacaaaatt gtaacagacc gcttccgggc ttgctcattc
     8821 aaaggacgcc ccttaagaaa agcgcgctcc cagcctctcc gtctaagatg cagcatcgaa
     8881 cggtgacgca ctcggcgcca gcataaccgt actcttccct tcgctaccat tgccgcggcc
     8941 acggagagca tgtgatattc tgtggcgccg aaaagaggct atcgaatgag ctatattgaa
     9001 cgcatccaga acgatcttac cgcagccatg aagggaaagg atgaattgcg gctgagcgtg
     9061 ttacgcatgg taaagagcgc cctgaagaac aaggaaatcg aaaagatgcg cccgcttgag
     9121 gacatggagt ccctccagat actccaaacg ctcgtcaaac agcggcgcga atccatcgat
     9181 cagttcaggc aaggcggccg aaaggatctg gccgagaagg aagccaagga aatcgccatc
     9241 atcgaagagt atctaccggc tgcgccaagc gatgaggaag ttcgccgagc ggtcgaggag
     9301 gccatttccg aaaccagcgc cggttcggtg aggcaaatgg gcgcggtgat caaggcggct
     9361 cgagcgaaac tggaagggaa agcagtggac ggcaaggttc taagtgatca tgtgcgcgaa
     9421 cgcctctcgg agaaatcctg acggctccga gtgagtccag attgttttta atcccgaaga
     9481 cgaagagcat ttcggttgcg gaaatcgccg tccctcgcgg caacgctcct gctctaagcc
     9541 tgcttcaacg cgtccggttg tccattggcc gcggcgatta gaggattatt tcccgccatt
     9601 ttcggcgagt cctgccacgt gctgctgaag gtcacgcgtt attgcttcgg caggacgtcc
     9661 ggcatcgatt gagatgggtg cgccgagcgt gatttttatt tggcccggag ccgcgcgcct
     9721 cttccctgct tgcttcaacg caaataagcc atccagccga gccggaacta ccggcgcgcc
     9781 aaggcctgcg gcgagaaggc ctactccggc gcgaaacgga gcgatgcggc catctcgcgt
     9841 gagctcgcct tcgggaaata tgaggacgct ccatccgcga tctattaaat cgcctgcgaa
     9901 ggcaaaactt ttgcggaacc cggcttgccg cggcaggggg aaaacgttga agagcgcgag
     9961 tgccaggaaa tactgtgtcc ggttgagcag accggagaaa aattcgaggt cgggcgaggg
    10021 gttgcgcatc gattccaggc gctctccatc catcgccacg gcgaggcggt ggcgcaggcg
    10081 cgcaggcagg gcgtgaagga tgagaacggg atcaaaatag gtgacgtgat tcgagatcac
    10141 gagcgttggc gcttttacat tgcgaaggtt ttcgcggcca cttacgcgcg gccatccgag
    10201 tattagaagc gcggggcgaa ccagcactgc gaaaaccgcg gctcgaagca gacgcgctgg
    10261 ccagctttgt ggccagcggg gatagtgaac ggttacggcg cctcgcgagg gctgcgcgat
    10321 caatttttcg agggcttcga tagtatttgc cgaggcaaat tccgtctcgc tcaaatccac
    10381 ctgatagcga tcttcgaggg tgctgagcag ttccacgcgc tcgattgagc ctagctgaag
    10441 agaggcggac tcgtttgatg gcaggcggcc gcgcgaaatt cctttaagga gttcgccgat
    10501 ggatccggat ggtgccgctt gagatgaacc ggatcgccgg ccaccgagtg ccgcttcggc
    10561 ggcctcgcga atgcggccaa gagccggctt ttgggttgga gtgcgaggaa agtccgcgtc
    10621 gggccagacg aaccagtgct gcatacgttg aaaaggcgcg aggcgctcgt tggcgcgctg
    10681 gacagtggcg gccgcctccg atgtgttcgc cgcatctttc agaatgagta cggcacacgg
    10741 ctgggcgttg ccatcacgct caagggcgat gaccacgcaa tcgcgcacgg aaggttcctt
    10801 gcgcagctct acttccaggt cttggggaaa aatgttcagg ccctcgggcg tgacgattac
    10861 atttttcctg cggcctttga aataaagatt gccggcttcg tcgcgttcgc cgatatcgcc
    10921 ggtgtg
//
LOCUS       DATNDT010000339         2366 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3343988, whole genome shotgun sequence.
ACCESSION   DATNDT010000339 DATNDT010000000
VERSION     DATNDT010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2366
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3343988"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="VN788_10965"
     CDS             <1..639
                     /locus_tag="VN788_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HXU21092.1"
                     /translation="SDAIVLFNGKDLSAWTGHPSPITHAGGTGEPEWKIENGYTEIVP
                     HTGDIATKEKFGDCQLHVEWSEPADITGRSQGRGNSGILFMGRYEIQVLDSYRSPTYA
                     DGQAGAIYGQWPPLVNAMRKPGEWQTYDIVFMAPKFEGGKLVSPVYFTVFLNGVLLHN
                     HKASMGPTVYRQVAHYVPQPAEDSLVLQNHNSRVRFRNIWIRRLGGYDEPEK"
     gene            complement(757..900)
                     /locus_tag="VN788_10970"
     CDS             complement(757..900)
                     /locus_tag="VN788_10970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21093.1"
                     /translation="MRKRLVVWMTVAGIGVAGVVQRQPRTRSFHFEYKGRNRWEAGAE
                     NGV"
     gene            complement(1207..1377)
                     /locus_tag="VN788_10975"
     CDS             complement(1207..1377)
                     /locus_tag="VN788_10975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21094.1"
                     /translation="MKKLMSVMLGLGFLVGSVSMFAQDTNTDSTKMEKKAKKAKHHKK
                     AKKDTTTPPATK"
     gene            1610..1819
                     /locus_tag="VN788_10980"
     CDS             1610..1819
                     /locus_tag="VN788_10980"
                     /inference="COORDINATES: protein motif:HMM:NF012442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HXU21095.1"
                     /translation="MGKKEIAERLARQAHLSQGDAADQLDRLVYEILQQVRKGHSATI
                     PGLGTFRAGDVLGFDLKGSGKGKKQ"
     gene            1816..>2366
                     /locus_tag="VN788_10985"
     CDS             1816..>2366
                     /locus_tag="VN788_10985"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXU21096.1"
                     /translation="MSVRYAGHSLRIARIVRRCLAEGASVEIDGLGVFQPSSGKPGFR
                     FVARNRPRVFLAYAAEDLTTVRKLYSALATAGFDPWLDKKKLLPGQNWPRSIETAIEN
                     SDFFIACFSRRSTAKRGCFQSELRYALECANRVAFDEIYFLPVRFEECEVPPRIARHL
                     QYVSLFPDWDEGVRALVQAMRDQ"
BASE COUNT          508 a          658 c          659 g          541 t
ORIGIN      
        1 tcggatgcga tcgtgctgtt caacggcaaa gatctttccg cgtggaccgg ccatcccagc
       61 cccattacgc acgcgggagg aaccggcgag ccggaatgga agattgaaaa cggatacacc
      121 gagattgttc cgcacaccgg cgacatcgcc acgaaagaga agttcggcga ttgtcaattg
      181 catgtcgaat ggtccgagcc ggccgacatc accggcagaa gccagggccg gggcaacagc
      241 ggcatactgt tcatgggccg ctatgaaatt caggttctcg attcttatcg aagtccaaca
      301 tacgccgacg ggcaggctgg ggcgatctac gggcaatggc cgccactggt gaacgcgatg
      361 cgaaagcctg gagaatggca gacttacgac atcgttttca tggctcctaa gtttgaaggt
      421 ggcaagttag taagtccagt ctatttcact gtctttctga acggcgtctt gctgcacaac
      481 cacaaggcat caatggggcc gacagtctac cgccaggttg cgcattatgt tccgcagccg
      541 gccgaagact cgctcgtttt gcagaatcac aattcgcgcg tgcgatttcg caatatctgg
      601 atcaggcggc tgggcgggta cgacgaaccg gaaaaataat cccgttttaa gccgctctaa
      661 agtgcgcacg caagcgcttg cgtgtgcact tttgggcgtc atttaacgcg ttaatttaac
      721 cctcatttcg cgccgccggc gtcccgatac gtgatttcat accccgtttt cggcgccggc
      781 ttcccatcga tttcggccct tgtattcaaa atggaaactg cgcgtgcgcg gctggcgctg
      841 aaccactcca gcgacgccaa ttccggcgac cgtcatccac actacgagcc tcttcctcat
      901 gccttgttcc tcgtattttt ggaatttgga tacaccaatt gcgcggagtt gcgcgatcgc
      961 gacactggcg gggattagcg gcgtgaaccc ggcaaatgcc gggccgggag ggcattcatg
     1021 gcccccctgg gccggactag cggagtgaat tccgcagtga gaggaaacag cagaacgctg
     1081 gccgacacat tggagagaat atcagttccc tatcctgcca accagccctc aaaaaaactt
     1141 atcgcgcatg acccacgcca aagcgcgaaa tcatgcgcga taaaaaattc gggctgaaga
     1201 accagactac ttcgttgccg gaggagtcgt ggtgtccttc ttggccttct tgtggtgctt
     1261 cgccttcttg gccttcttct ccatcttggt cgagtcagtg ttggtatcct gcgcgaacat
     1321 cgagacggat ccgacgagga acccgaggcc cagcataacg ctcatcagct ttttcatttg
     1381 atattttctc cttggtagat tagggggtaa caaactttac gacttaatca tgtcaatccg
     1441 acatgaagcg gtccttaaga gcctattaaa aacacttcat cgttgccagt cacctcactg
     1501 ccaattttct ttcgagttgg taagtttatc cggctcaaga agttgtatgt ggtttgcact
     1561 cagcccctag ggccgattcc ctagagtctc acggctatga tgacaccgta tgggaaagaa
     1621 agaaattgcg gagcggctcg ctcgtcaggc gcacctctca cagggcgatg cggcggatca
     1681 attggatcgg cttgtgtatg aaatattgca acaagtccgc aaagggcata gcgcgacaat
     1741 accgggcctg ggcacatttc gagctggcga cgtgcttggc ttcgacctga aaggttcggg
     1801 gaaaggtaag aagcagtgag cgtgcgctac gccggacaca gtctccgcat cgcccgtatc
     1861 gtgcgccgct gtctggccga gggcgcttcc gtcgagatcg acggtctggg tgtttttcag
     1921 ccgtcttcgg gaaagcccgg tttccgtttc gtcgcgcgca accggccgcg cgttttcctt
     1981 gcttatgcgg cggaggacct gaccacggtc cgcaagctct attccgctct cgcgaccgcg
     2041 ggttttgatc cgtggctcga taaaaagaag ctgcttccgg gtcagaactg gccacgatcg
     2101 attgaaacgg cgattgaaaa cagcgatttt ttcatcgcct gcttctcccg tcggtccacc
     2161 gccaaacgcg gatgcttcca aagcgagctg cgctatgcgc tcgagtgcgc taaccgcgtg
     2221 gcgttcgatg aaatttattt tcttccggta cgcttcgagg aatgcgaggt ccccccgcgt
     2281 atcgcgcgcc atctgcaata tgtcagcctc tttccggatt gggacgaggg agtgcgggca
     2341 ttggttcagg ccatgcggga ccagat
//
LOCUS       DATNDT010000340         4952 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_88194, whole genome shotgun sequence.
ACCESSION   DATNDT010000340 DATNDT010000000
VERSION     DATNDT010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88194"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..491)
                     /locus_tag="VN788_10990"
     CDS             complement(111..491)
                     /locus_tag="VN788_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017307576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1634 domain-containing protein"
                     /protein_id="HXU21097.1"
                     /translation="MADVRGLSEKKFEALIGMLLRSGVVLAATVVLAGGVLYLTKYGG
                     LKPDYRIFRGEPSDLRSIGGVVGDAISLHSRGIIQLGLLLLIATPIARVAFSVAGFAS
                     EHDWLYVGITLVVLGVLIYSLLSS"
     gene            complement(948..1787)
                     /locus_tag="VN788_10995"
     CDS             complement(948..1787)
                     /locus_tag="VN788_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HXU21098.1"
                     /translation="MHGTVEFTLLIAGGSFLAGLLGSLTGLGGGVIIVPLLTLAFGVD
                     IRYAIGASLVSVIATSSGAAAAYLKEGFSNIRVGMFLEVATTIGAVCGAYLATRIPTA
                     AIAIIFGAVLLYSAWISVKPEDEVHIHEKTGSLARVLGLDGNYPTPEGTMAYHVAGVP
                     LGFSLMYVAGVLSGLLGIGSGAVKVLAMDRAMKIPFKVSTTTSNFMIGVTAAASAGIY
                     LHRGYIDPGLAMPVMLGVLAGALVGARILPEAKVKTLRIVFGVAIAAVAIEMIVKGVR
                     GNL"
     gene            complement(1988..2308)
                     /locus_tag="VN788_11000"
     CDS             complement(1988..2308)
                     /locus_tag="VN788_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013033562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21099.1"
                     /translation="MKQKIEVEIASEGTFRVRVIEGETETLHAVTLKQADYERISGGK
                     IEPAELVRMAFEFLLENEPKESILGRFDLPLIGRYFSNFEPEIGRRLASRSPRWEPHR
                     SRRI"
     gene            2429..2896
                     /locus_tag="VN788_11005"
     CDS             2429..2896
                     /locus_tag="VN788_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21100.1"
                     /translation="MATWGVGFPQVEVASSRVVEIAREMAESDQEQRRVDQFILEKID
                     SVSHLEALLLLWNSRPKQWSPEELARRIYVDIKIARELLADLQRSDLIVAAPDAAEKY
                     WYETSSQERNGIIEIVDATYRYQIVRISNLIHSKGPSAVRDFARAFRFKKEPE"
     gene            2899..3156
                     /locus_tag="VN788_11010"
     CDS             2899..3156
                     /locus_tag="VN788_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HXU21101.1"
                     /translation="MAEAVYLLGALTSLLCAILLLRGYATGKRKLLLWSGLCFVGLGV
                     SNGITFIDLILLPQVDLYSYRLAATAISTALLLYGLIWNSQ"
     gene            3153..3434
                     /locus_tag="VN788_11015"
     CDS             3153..3434
                     /locus_tag="VN788_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5985 family protein"
                     /protein_id="HXU21102.1"
                     /translation="MTKIQAFLLGITVMMSTTAGVFFLKFWRSTRDVLFLAFAAFFLL
                     EALDRTTLFIFARDNEGSPWIYAIRLLATLAILAAILRKNYGSGRSRTL"
     gene            complement(3490..>4952)
                     /locus_tag="VN788_11020"
     CDS             complement(3490..>4952)
                     /locus_tag="VN788_11020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXU21103.1"
                     /translation="GLIGGEVGLGLAYGGLRLLVAIGPTNLPRLGEISVDPLVLAFAL
                     GASLLSGLFFGFVPAWKNSGTRVATDLHGGGRTASQSREHHHARNVLVVVQMALALVL
                     LVSAGLMIRTFQALRSVQPGFLRPEEIQTIRITIPESQVQDPDQVARMESAIRDKIAE
                     IPGVSSVGFSSSMPMEGLQSGDAVWVEDKTYPPGQFPPGRSFKWVSPGYLQSMGTRLI
                     AGRDITWTDVYDHRPVAMVSENMARELGGKPAAALGKRIRIAGPGPPRWREIVGVVED
                     VRDDGVHQKAPSLVYWPVRMENFWGPEFIMRSVAFSIRSKRAGNEGFVNEVHQAVWSV
                     NPNLPLALVRTMQQVYDRSMAGTSFALVMLGIAGATALVLGIIGIYGVISYAVSQRTR
                     EIGIRVALGAQRRDIIRIVMAQGGTVALVGIGLGLAASFGLTRLMATMLFGVSATDPL
                     TFAAVALILLAVALLACYVPARRAMRVDPMVALRHE"
BASE COUNT         1093 a         1570 c         1268 g         1021 t
ORIGIN      
        1 cggcgggcgc gttcgcttcc aaaattatca aaatggtgat gaatgcaagt acgttggcac
       61 tgcggagtga aagcctcatc ttgtcccttc gtcttctgat gcgagctgcc ttaactgctc
      121 aataaactgt agatcagcac cccaagcacc accaaggtaa ttcccacgta aagccagtcg
      181 tgctccgatg caaaccccgc cacggaaaat gccacgcgcg cgatcggagt tgcgatcagc
      241 agcagcaacc caagctggat gatcccgcgc gaatgcaacg aaatcgcatc tccaacaact
      301 cctccaatgg atcgcaaatc gctcggttcg ccccgaaaaa ttctgtagtc gggtttcaag
      361 cccccatact tcgtcaaata caaaacgccg cccgcgagca ccaccgtcgc ggcgagcaca
      421 actccgctgc gcagcagcat cccaatcagc gcctcaaatt tcttttcact caagccacgc
      481 acgtcagcca tcttcacctc gcaccaaaat cgccgctttc gtaggggcgt agcactgcta
      541 cggccgccaa ccatgcttgg ctacttgctc accatccgcc tcagccatct tcacttcgca
      601 ccaaaatcgc cgcttttgta ggggcgtagc actgctacgc ccgccaacca tgcttggcta
      661 cttgcttacc atccgcctcg tccgaacggg cgcacctatg tggagtgcgg cggcttgttt
      721 accctgagct tcgaagggcc gccgctgttt gccgaccggg cgcctgtctc ttttcttcac
      781 gcaatgtcat cgctccgctc cccaaaagcg gtcgtcatcc tgagcgaaat cccgcgcttt
      841 tttcttcgcg cggtttgtgc gcggcgcggg tcgcagtcga agaatctctc ttcgatctgc
      901 caactgactc tttgcccgca aacatggact tttcgcacca aacgccgcta caaattcccg
      961 cgtacgcctt taacaatcat ctcaatcgcc acggccgcga tcgccacacc aaacacaatc
     1021 cgcaatgttt ttactttggc ttccggcaaa atccgcgccc ccaccaacgc ccccgccagg
     1081 acccccaaca ttacgggcat cgccagcccc ggatcgatgt atccccgatg caaataaatc
     1141 cccgcgctgg ccgccgctgt caccccaatc atgaaattgc tcgtggtcgt cgaaaccttg
     1201 aacggaatct tcatcgcgcg atccatcgcc agcaccttca ctgcgccgga cccgattccc
     1261 agcagccccg agagaactcc cgccacatac atcaacgaaa atcccagcgg tactccagcc
     1321 acgtggtacg ccatcgtgcc ttcaggcgtt ggataattgc cgtcgagccc cagcacacgt
     1381 gcgagcgatc cggttttttc atggatgtgt acttcgtctt cgggcttcac ggaaatccac
     1441 gcggagtaca acagcacggc tccaaaaata atcgcgatcg ccgccgtcgg tatgcgcgtc
     1501 gccagatacg caccgcacac cgccccgatt gtcgtcgcaa cttcgaggaa catgccgacg
     1561 cgaatatttg aaaatccctc tttcaggtac gcggctgcgg cgccggatga agttgcgatc
     1621 acggagacca gcgaggcacc aatggcgtag cgaatgtcca cgccgaaagc cagggtgagc
     1681 agcggcacga tgatcacgcc gccgccgagt cctgttagcg agccgagcaa ccccgccaaa
     1741 aacgatccgc cggcgataag cagcgtgaat tcaaccgttc cgtgcacagc ccgattctaa
     1801 tggtgagccc accttctgca atcggaatct gccagtcacg cgcacacccg ggcactcagc
     1861 aacactcttc gaccgtcaac ctcgagtgaa gtaaaagcga aaaagagtca cattttttac
     1921 ctctagttcg aagcagaaaa tccttctaac tcgaaacatt aagggggcca aaaacgtcgt
     1981 cgcggtctca gatcctccgg ctacggtgcg gttcccaccg gggcgagcgg ctcgccagac
     2041 gccgcccgat ttcgggctcg aaattcgaaa aataacggcc gatgagcggc aggtcgaacc
     2101 gccccagtat cgattccttg ggctcgttct ccagcaaaaa ctcaaacgcc attcgcacga
     2161 gttcggccgg ctcgattttt ccgcccgaga ttcgctcgta gtcggcttgc ttgagcgtta
     2221 cagcgtgtaa cgtctcggtc tcgccttcga tcacgcgaac gcgaaacgtt ccctcgctgg
     2281 caatttcaac ttcaattttc tgtttcatcg gcgcgctccg aatctttcgt ctattgatcc
     2341 gagatgatct cacagaaacc gcccaaattt cggatcagcg aattcaattt cggcgtctac
     2401 agaatttacc gttgcttttt taggcgcgat ggcgacgtgg ggcgttggct ttccgcaagt
     2461 ggaagtggca tcatcgcgcg ttgttgagat tgcgcgagaa atggcagaaa gcgatcaaga
     2521 acagcgccgg gtcgatcagt tcattctaga gaagattgac agcgtctctc acctcgaggc
     2581 ccttctcctt ctgtggaaca gccgccctaa gcaatggtct ccggaagaac tggcgcgccg
     2641 gatttatgtg gacataaaaa ttgcgcgaga gcttttagcc gatttgcaac gaagcgatct
     2701 gatcgttgct gctcccgatg cggcggagaa gtattggtac gagaccagct cccaagagag
     2761 aaacgggatc atagaaatcg ttgacgcgac gtatcgctat caaattgtta ggatctcaaa
     2821 cctgattcat tccaagggac cgtccgccgt tcgggatttt gcgcgcgctt ttcgatttaa
     2881 aaaggagcca gagtagccat ggccgaagcc gtgtatcttc tgggcgctct cacaagtttg
     2941 ctgtgcgcta tcttgcttct ccgcggatat gccacgggaa aaagaaagct gctgctgtgg
     3001 agcggattgt gttttgtggg cctgggagta tcgaacggaa ttacttttat cgacctgata
     3061 ctgctgccgc aggtggacct atactcctac cggctggccg ccacggcgat ttccacggcg
     3121 ctgctgcttt acggcttaat ttggaatagc caatgactaa aatccaagcg ttcttactcg
     3181 gaattacggt gatgatgtcg accacggccg gtgtgttctt cctgaaattc tggcgaagca
     3241 cgcgggatgt tctcttcctg gccttcgcgg catttttcct gctggaggcg ctggatcgca
     3301 cgacgctttt tatctttgcg cgcgacaatg aaggaagtcc ctggatctac gcgattcgat
     3361 tgttggccac gctagcgatc ctggccgcca tcctgagaaa gaactacggc agcggccgct
     3421 cgcgaactct ctgaacgaac tcctcttcca aattgcggcg cgaggcctcg ccgactctct
     3481 tccagaaact cattcgtggc gcagggcgac catgggatcg acgcgcatcg cgcggcgcgc
     3541 gggcacatag caagcgagga gcgcgacggc cagcagaatc agggcaacag ctgcaaacgt
     3601 cagcgggtcc gtcgcgctca caccgaatag catggtcgcc atcaggcgag tcaatccgaa
     3661 ggacgcggca agtcccagcc cgatgcctac cagtgctact gttcctccct gggccatcac
     3721 gatacggatg atgtcgcgcc gctgtgcgcc tagcgccacg cgaattccaa tctcgcgcgt
     3781 cctttgcgag accgcgtacg aaatgacgcc gtatatgccg ataatcccca acaccaatgc
     3841 cgtcgcgccc gcgatcccca acatcaccaa tgcgaacgaa gtgcctgcca tcgaccggtc
     3901 gtacacctgc tgcatcgtcc gcaccaatgc caatgggagg ttcggattca ccgaccaaac
     3961 cgcctggtgg acctcgttta cgaagccttc attgccggcg cgcttactgc ggatcgaaaa
     4021 cgccaccgag cgcatgatga actccggtcc ccagaagttc tccatacgga ccggccagta
     4081 cacgaggctg ggagctttct ggtggacacc gtcgtcgcgc acatcttcca cgacgcccac
     4141 gatttctcgc caccgtgggg gtcccggccc ggctatgcgg attcgtttac ccagagccgc
     4201 agccggttta ccccccagct cgcgcgccat gttttcagac accatcgcca cgggtctgtg
     4261 gtcgtacaca tcggtccaag tgatatcccg ccctgcaatc agtcgcgttc ccatgctctg
     4321 gaggtacccg ggagaaaccc acttgaacga gcggcccgga gggaactgac cgggagggta
     4381 tgtcttgtct tccacccata cggcatctcc actttgtaat ccttccatgg gcatggagct
     4441 ggaaaagccg accgaactga caccgggaat ctctgcaatc ttgtcccgaa tagcgctctc
     4501 cattcgagcg acctggtccg gatcctggac ctgggattca ggaatcgtaa ttcgaatcgt
     4561 ttgaatctcc tctggccgta agaatcccgg ttgaacgcta cgcagagcct ggaatgtgcg
     4621 aatcatcagc ccggcgctca ccagaagcac caatgcgaga gccatctgaa ctaccaccag
     4681 cacgttgcga gcatgatgat gttcccggct ctggctcgca gtgcggccgc cgccatgcaa
     4741 gtccgttgcg acgcgagttc cagagttctt ccatgccggg acgaatccga aaaagaggcc
     4801 ggaaagcagc gaggcaccga gagcgaacgc caacaccaac ggatcgaccg aaatctcgcc
     4861 caggcgcggc aggttcgtcg gaccgatggc tacgaggagc cgcagtccgc cgtatgccag
     4921 gcccagtccc acctcgccgc caatcaaacc aa
//
LOCUS       DATNDT010000341         7999 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3519590, whole genome shotgun sequence.
ACCESSION   DATNDT010000341 DATNDT010000000
VERSION     DATNDT010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7999
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3519590"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VN788_11025"
     CDS             <1..400
                     /locus_tag="VN788_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21104.1"
                     /translation="KLSLVGDSMHASMQSVQSAREAHAYRLNAAREHINNAGRLLAEL
                     QGMYPELAPWQQQAVKRITPIASEIAEHTQAAIVHLNERPAARLAPAYTNHLQGIVDQ
                     SNSMRETVGNFLDYADAKSTAQMLETTLEI"
     gene            complement(493..1401)
                     /gene="nhaR"
                     /locus_tag="VN788_11030"
     CDS             complement(493..1401)
                     /gene="nhaR"
                     /locus_tag="VN788_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HXU21105.1"
                     /translation="MEWLNYHHLYYFWTVVRKGSIAKACEELRLAPPTVSAQLRDFED
                     HLGEKLLERSGRGLVPTEMGQRVYRQAEEIFSIGQEIMDTVKHRPTRRPMRLAVGIDD
                     VLPKIVAYRIIEPALHLKVPVQLWCREGTLEQLAAGLAAHEFDVVLSDAPVTPTMNVQ
                     VYSHQLGTTGVVWMGARHLARIYARGFPKSLDGAPILLPTDDTAIRLRIDQWFASLGV
                     RPNVVGEFEDYALLRAFGHGGAGVFPVPAILEREYHREGNLRRIGVARNILEHVYAIS
                     AQEEVRHPGVVAIYENAARRAFARSR"
     gene            complement(1477..2154)
                     /locus_tag="VN788_11035"
     CDS             complement(1477..2154)
                     /locus_tag="VN788_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21106.1"
                     /translation="MSTVFEERFHKSGSPLDGAQSKNRLRPAPNGRTQNNPSGSQLLT
                     DLLSAVPVDLARISEHIRSNAEIAELVVGLAGLLLLSAGESRLTIENAAVILGTDRLR
                     VMASMWSLIHEHGEERGAEKPAGLAAGPGDRWRTCCIHGTAWTAQTLYLAAFLRWLGL
                     DSPDSELPKDCTQFAAPTWQPEDFADLRNLLMRDFLALLPTLNAPSSKADPATPATGG
                     TKPSTKQ"
     gene            complement(2175..3065)
                     /locus_tag="VN788_11040"
     CDS             complement(2175..3065)
                     /locus_tag="VN788_11040"
                     /inference="COORDINATES: protein motif:HMM:NF005413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HXU21107.1"
                     /translation="MHVHTDMYTTLLDEHECEKLLLDHLPHVQYVARRIHTRLPPQVL
                     LEDLVHAGILGLMDAVRKYDPSKNVQLKHYAEFRIRGAILDSLRLVDWSPRALRRQAR
                     RLEQAISRCKARLGRDPSESEIATELEVSLDNLQHLRGDLRGLDIESLQSTAEDHAGE
                     PPDSQARSEEDDPYQQALRSEMNSLLELAITELPPREREVLALYHFQELTMKEVGEVL
                     RIGESRVSQIHSAALSRLRARLLEIVAKPPQTQTQTQTATVRRTEVATQQKPALMEAA
                     KTNNASPLRASRSPLHEMVS"
     gene            complement(3094..4326)
                     /locus_tag="VN788_11045"
     CDS             complement(3094..4326)
                     /locus_tag="VN788_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU21108.1"
                     /translation="MSAVTQPLWRELKGKVLVASPYAPLREQMLKKFAVPNASVSEAR
                     GGADALMKLEKSDFGTLLLDPRIEDLDVGELMATLQGRYPNLDVVLMESDADRDEEPD
                     DAKPAQASATANLLSARSTAPATYPPPVYRPYPFEIATETFGSTEPRTHALPGMIGTS
                     LGIQQIYRLARLVAPRDTAVLIVGETGTGKELVARGIHDLSRRSKGPFMVVNCAAIPE
                     SLLEAELFGFTRGAFTGAFQSRLGRIHAAHGGTLLLDEVGELPLGMQAKLLRFVQEGE
                     VQRLGSADVFRVDARLIASTNANLLQRVREKQFREDLYYRLSVFPVDLPPLRERTGDT
                     LPLAQHFLDGFCRSSGVTPKVFSPEAASLLQRYHWPGNVRELQHSVERAFVLAEDNPW
                     IHVENLAMLTGAPDLTKF"
     gene            complement(4360..5046)
                     /locus_tag="VN788_11050"
     CDS             complement(4360..5046)
                     /locus_tag="VN788_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU21109.1"
                     /translation="MRILVAEGDAALGEFLEREFGEEHYSVDLASDVAEITRLAEQRD
                     YQAAILDFNVEGPRCLASLRQIRPHDQLPILVLASHAKPEARAQVLDLGADDLLLKPF
                     AFSELAARVRALVRRGGHGPDTVLRVDDLELSRVERRVIRAGRSIELTPKEFALLEFL
                     MLNTSHPVSRPQIVERVWNLSFDTMTNVVDVYINYLRKKVDTPSDGKLIHTVRGVGYQ
                     LQARNQHFAA"
     gene            complement(5269..6684)
                     /gene="fumC"
                     /locus_tag="VN788_11055"
     CDS             complement(5269..6684)
                     /gene="fumC"
                     /locus_tag="VN788_11055"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015153682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HXU21110.1"
                     /translation="MDAKASSASKAGARVESDSMGKIEVPNDRYYGAQTARSLIHFNI
                     GRDTMPPELIRAFGILKKAAALVNEDLKKLSPEKARLIVQAADEVIAGKLDEHFPLRI
                     WQTGSGTQTNMNANEVISNRAIEISGGVMGSKKPIHPNDDVNMSQSSNDTFPSAMHIA
                     AASRIAEALIPSVAGLRDALAAKAREFANVVKIGRTHLMDATPLTVGQEMSGWVSLLD
                     RDLERLEKILPGLYDLAIGGTAVGTGLNAHPEFAQRAAKKISELTNLPFTSHPNKFAA
                     LSAHDELVFASAALKTLAASLMKIANDIRWLASGPRAGLSELRLPENEPGSSIMPGKV
                     NPTQCEAMTMVAVQVFGNDAAIGFAGSQGNFELNVFKPVIIHNFLHSVRLLADACRSF
                     ADHCVSGMELDLQRIEQYVKNSLMLVTALSPIIGYDKCAAAAHKAHHEKSSLRQAVLE
                     LGYLTADEFDQIVVPEKMTHP"
     gene            6908..>7999
                     /locus_tag="VN788_11060"
     CDS             6908..>7999
                     /locus_tag="VN788_11060"
                     /inference="COORDINATES: protein motif:HMM:NF037667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HXU21111.1"
                     /translation="MPLTMHGKQSDSGGRKTRRLATSTAILSLVLLASTAWGTRFAAR
                     ADEPYAPSRDYDLQDIRIHLWLDGTGPQFRGEVTERVAALRNGIGEFKFDSVGLNIQD
                     VTVDGASSKFSTTPTQLIVALAHPTTRGERHEILIRYSGAPKRGLYFILPNQDYPHQP
                     AEVWTQGEAEDTRYYIPLYDYPNDRATSEMLLTVPSSWITVSNGQLLGVKNEAGGAKT
                     WDWKQSQPLSTYLISAIAGEFDEHDDSWNGVALRFLVPRGNQSEIPSTFARTKQMLDL
                     FSSKLGVAYPWGQYAQTSVDGFTEGGMENTSATTLSVRDLVNPKLLPELRIGDDFVIS
                     HEMAHQWFGDLVTCKDWANLWLNEGFATFF"
BASE COUNT         1696 a         2271 c         2351 g         1681 t
ORIGIN      
        1 aaaactcagc cttgtcggcg actcgatgca tgcatcgatg caatccgtcc aaagcgctcg
       61 cgaggctcac gcgtaccgac tgaacgccgc gcgcgaacat attaataatg ccgggcgttt
      121 gctggctgaa ctgcagggca tgtatccgga gcttgcgccc tggcagcagc aggccgttaa
      181 gaggataacg ccaatcgcat cggaaatagc cgagcatacg caggcggcaa tcgttcatct
      241 gaacgagcgg ccagcggcac ggctggcgcc ggcatacaca aaccatctgc agggaattgt
      301 cgaccagtcg aattcgatga gggagacagt tgggaacttc ctggactatg ccgatgcaaa
      361 gagcacagcg cagatgctcg agaccacgct tgaaatctga ccgcttagaa aacaggaccg
      421 cgagagggag cctaccgttt caccaccacg catggagaac gggttcggct ccctctggcg
      481 ctgtgccagc gatcagcgcg atcgggcgaa agctcgccgc gcagcgtttt catagatggc
      541 gaccacgcct ggatgccgta cctcttcctg cgccgaaatc gcgtagacgt gctcgaggat
      601 atttcgcgcg acgccgatcc ggcgcaagtt gccttcgcga tggtattcgc gttctaaaat
      661 cgccggcaca gggaacactc cagcgccacc atgtccgaat gcacgaagca gcgcgtaatc
      721 ctcaaattcg ccgactacat taggccgcac tccgagcgac gcgaaccatt gatcgattcg
      781 caggcgtatc gccgtgtcgt ccgtgggaag caggatcggc gctccatcca atgattttgg
      841 aaagcctcgc gcgtatattc ttgccaggtg ccttgcgccc atccacacga cgccagtcgt
      901 ccccagttgg tggctgtaga cttgaacgtt catggttggc gtgacggggg cgtccgacag
      961 aacgacgtcg aactcgtgcg cggcaaggcc ggccgcgagc tgttccagcg tcccttcgcg
     1021 acaccagagc tgaacaggaa ctttgaggtg taacgcaggt tctatgatgc gataagcaac
     1081 gatcttgggc agcacatcgt caattcccac tgcgagacgc atggggcggc gcgtggggcg
     1141 atgcttgacc gtatccatta tctcctgccc gatggagaaa atttcctcgg cctgccggta
     1201 cacgcgctgg cccatttccg tgggcaccag tcccctgcct gaacgctcca gtaatttctc
     1261 gccgagatga tcctcgaagt cgcgcagttg tgcgcttacg gtcgggggag ccaatcgcaa
     1321 ttcttcgcaa gctttcgcta tgcttccctt gcggacaacc gtccagaagt aataaaggtg
     1381 gtggtaattt agccactcca tgccgggaat gctactacgt taccaaagcc ggcgcggcac
     1441 aagagttgcg cccgagggag gcatcggtgg atgcttctat tgcttcgttg aaggctttgt
     1501 gccgccggtg gcgggggttg ccggatcggc tttcgatgaa ggtgcattga gggttggaag
     1561 gagcgcgaga aagtcgcgca ttagaagatt cctgaggtcc gcaaaatctt ccggctgcca
     1621 ggttggcgca gcaaactgcg tgcaatcttt gggtaattcg gaatcgggag aatccaatcc
     1681 caaccatcgg agaaaagccg ccagataaag ggtctgagca gtccaggctg tgccgtgaat
     1741 gcagcatgtt cgccaacggt cgcctggacc ggccgctaag ccggcgggct tttcggcgcc
     1801 acgctcttcg ccgtgctcgt ggattaggga ccacatagag gccatcacac gcaggcgatc
     1861 ggttccaagg atgacggcag cgttctcaat ggtaagcctg ctctcgccgg cagaaagaag
     1921 gagcaatccc gcaagcccga ccacgagttc ggctatttcc gcattcgatc gaatgtgctc
     1981 gctgatccgc gccagatcaa cggggacggc gctcagaagg tccgtcaaaa gttgcgagcc
     2041 cgaggggttg ttttgggttc gtccgttggg tgccggcctc agacgattct tggactgcgc
     2101 tccatcgagt ggagaccccg atttgtggaa ccgctcttca aagactgtgg acatacaatc
     2161 gctatgtcga atggctacga caccatctca tggagtgggc ttcttgaggc ccttaggggt
     2221 gaagcattgt ttgttttcgc tgcctccatg agggccggtt tttgctgagt tgcaacttcc
     2281 gtacgccgga cggtggccgt ttgcgtttgc gtctgcgttt gtggcggttt ggcgactatt
     2341 tcgagcagcc tggcgcgcaa gcggctgagc gcggcggagt gaatctgcga gacgcgagat
     2401 tcgccgatgc gcaatacttc ccccacttcc ttcatggtga gctcttggaa atgatagagc
     2461 gccagcacct cccgctcgcg gggcggcaat tccgtaatcg ccagctcaag caaagagttc
     2521 atttccgacc gaagcgcctg ctggtaggga tcgtcctctt ccgatcgggc ttgggaatct
     2581 ggcggttcgc cggcatggtc ttccgcagtg gattgaaggc tctcgatatc caggccacgg
     2641 agatcgccgc gaaggtgctg aaggttatcg aggcttactt caagctcagt cgcaatttcg
     2701 gactcgctgg ggtcgcggcc cagccgcgcc ttgcaccgcg agatcgcttg ctccagccgc
     2761 cgcgcctgac ggcgcagcgc ccgcggactc caatccacga gccggaggct atccagaatc
     2821 gcgccgcgga tgcggaattc ggcgtagtgt ttcagttgaa catttttgct tggatcgtac
     2881 tttcgcaccg catccatcag tccgagaatg ccggcgtgaa cgagatcctc gagcaaaact
     2941 tggggcggca aacgagtgtg aatgcggcgc gcaacgtatt gaacgtgcgg aaggtgatcg
     3001 aggagcagct tctcgcactc gtgttcatcg agcagcgttg tgtacatatc cgtgtggaca
     3061 tgcacggctg gttccaggaa cggccgaaca agattagaat tttgtaaggt cgggcgcccc
     3121 ggtgagcatg gctaaattct cgacgtggat ccaaggatta tcctccgcca ggacgaaggc
     3181 tcgctcaacg gagtgctgca gctcccgtac attacccggc caatgatagc gctgcaatag
     3241 actcgcggcc tcggggctga aaaccttcgg cgtaacgccg gacgagcggc aaaatccatc
     3301 cagaaaatgc tgcgccaggg gaagcgtatc gccggttcgt tcccgcagcg gcggaagatc
     3361 gactggaaaa acagataacc ggtaataaag atcctcgcga aactgttttt cgcggacacg
     3421 ttggagcaaa ttggcgttgg tggaagcgat cagccttgcg tccacccgga agacatccgc
     3481 gctgccgagg cgttgcacct caccttcctg gacaaagcgc agcagcttcg cctgcatgcc
     3541 aagtggcaat tcgcctactt catccaacag gagcgtcccg ccgtgtgccg cgtggatgcg
     3601 gcccagtctg gattgaaagg cgcccgtgaa agcaccccgc gtgaaaccga aaagctcggc
     3661 ttcgagcagg ctttccggaa ttgccgcgca attcacaacc atgaacggac ccttgctgcg
     3721 ccggctaaga tcgtgaatac cgcgcgcgac cagttccttg ccagtgccag tctcgcccac
     3781 gattagaacg gccgtgtccc gcggcgctac caggcgagcg agccgataaa tctgttgtat
     3841 tcccaggctc gttccaatca tcccaggcag cgcgtgcgtg cgcggctccg tcgagccgaa
     3901 ggtttcggta gcaatttcga agggataggg cctgtaaacg ggcggaggat aggtggccgg
     3961 cgccgtggac ctggcgctca gtagattggc cgttgcggaa gcttgcgcag gctttgcgtc
     4021 gtcgggttct tcatcgcggt cggcatcgga ttccataaga acgacgtcga gattgggata
     4081 gcggccctga agagtggcca tcagctcacc gacgtccagg tcttcgatgc gcggatcgag
     4141 cagaagcgtg ccgaaatcgc tcttctcgag cttcatgaga gcatcagctc ctccccgcgc
     4201 ctccgaaacg gaagcattgg ggacggcgaa ctttttcagc atttgctctc ggagcggagc
     4261 gtaggggctt gccactagca ccttcccttt tagttctctc cacagcggct gggtgactgc
     4321 ggacataaat ggcctcctga aattatgtag actcaacttt caggccgcga aatgctgatt
     4381 tcgcgcctgc agctggtaac ccactcctcg aaccgtatga atcaactttc catcggacgg
     4441 cgtatccacc ttcttacgca ggtaattgat gtacacgtcc actacgtttg tcatcgtatc
     4501 gaacgacaaa ttccaaacac gctcgacaat ttgcggccgc gacaccgggt gagacgtatt
     4561 gagcatcagg aattcgagca acgcgaattc cttgggcgta agttcgatgg agcgtccggc
     4621 ccgaatcaca cggcgttcca cgcggctcaa ttcgagatcg tccacgcgca atacggtgtc
     4681 cggtccatgc ccgccacgcc gaacgagcgc ccgtacgcgg gccgccagct cggagaacgc
     4741 gaaaggcttc agcagcaaat cgtccgctcc gagatccaac acttgtgccc tggcttcggg
     4801 tttagcatga ctggctaaaa cgaggatggg caattgatcg tgggggcgaa tctgtcgcag
     4861 ggaggcgagg caccggggac cctcgacatt aaaatcgaga atagcggcct ggtaatcgcg
     4921 ctgttccgcc aagcgcgtga tttcggctac gtccgaagcc aaatcgacgg aatagtgctc
     4981 ttcgccgaac tcccgctcca aaaactcgcc gagcgcggca tcgccctcgg ccacaagtat
     5041 ccgcatgaat gctccagcgg aagggggaca aatttttaaa agttcaagta cgcgtaaatt
     5101 tagttaaggg ttgagttagt gtcaaaggga tttatgtttt ctaaaattag accgttctag
     5161 aagtgagaat gcccgtacca agctgacgaa aagtgttagc cgccgcgcgc aaaaacggtc
     5221 cgaaccgaac cacaaatgcg ccaagttgga acagttttgg ggcgaatctt acggatgagt
     5281 catcttctcg ggtacaacga tttgatcgaa ctcatcggcg gtgaggtagc cgagctcgag
     5341 aacggcctgg cggaggctgg atttttcatg gtgagccttg tgggccgccg cggcgcattt
     5401 gtcatatcca atgatcgggc tgagagcggt gaccagcatc agcgaattct taacgtactg
     5461 ctcgatgcgt tgcaaatcca attccatgcc ggaaacgcag tggtccgcga atgagcggca
     5521 ggcgtccgct agcaggcgga cggaatgcag gaaattatgg atgatcaccg gcttgaagac
     5581 gttcaattcg aaatttccct gcgagccggc aaaaccgatg gcggcgtcgt tgccgaacac
     5641 ctgcacggcg accatggtca tcgcctcgca ctgtgtggga ttcactttcc ccggcatgat
     5701 cgaagatcca ggctcatttt cgggaaggcg cagctcactt aagcccgcgc gcggccccga
     5761 cgccagccag cgaatatcgt tggcgatttt catcagagaa gcggccagtg ttttgagcgc
     5821 tgcgcttgcg aaaaccagct cgtcgtgcgc ggaaagcgcc gcaaatttat tgggatggga
     5881 agtgaacggc aggttggtca actcgctgat ttttttggca gcgcgctgag caaattcagg
     5941 atgggcgttc aagccagtgc ccacagccgt tcctccaatg gcgagatcgt ataatccggg
     6001 cagaattttc tcgagacgct ccaggtcacg gtccagcagg ctcacccagc ccgacatttc
     6061 ctggcccacg gtgagcggcg tggcgtccat tagatgcgtg cggccgattt tcaccacatt
     6121 cgcaaattcg cgcgccttgg cggcgagagc gtcgcgcaat ccggccacgg acggaatcag
     6181 ggcctcggca atacgcgacg ccgcggcgat atgcatggcg ctcggaaaag tatcgttgga
     6241 agattgcgac atgtttacgt cgtcgttggg atgaatcggt tttttgctgc ccatcacgcc
     6301 gccggaaatt tcaatggcgc gattggaaat cacctcgttg gcgttcatgt tggtctgcgt
     6361 gccgctaccg gtttgccaga tgcgcagcgg gaaatgctcg tccaactttc ctgcgatcac
     6421 ttcatcggcg gcctgaacga tcaggcgggc cttttccggc gaaagcttct taagatcctc
     6481 attcacaagg gcggcagcct ttttcagaat tccgaaggcg cggatcaatt cgggaggcat
     6541 ggtgtcgcgg ccgatgttga agtgaattag cgagcgggcg gtctgggcgc cgtaatagcg
     6601 gtcattcggc acttctattt tgcccatgct gtctgattcc actcgtgctc ccgctttcga
     6661 tgccgaagac gctttggcgt ccacgctggc ccccttcgtc aagatattcc gaaatggcta
     6721 gagaattctc gggattcaaa cttagcatga tctccgggcg atcgttgcag gaatcgccgc
     6781 gagcatcctg cagaagtcct ggcggcatct taggcgagcc ggcatcgccg cagttcggcg
     6841 cacggtacag gccgtgaaag cgcggtccgg tgcgttcggt tatccggcta taataattgg
     6901 ctaacgaatg cccttaacaa tgcacggaaa gcagagtgac agtggcggcc ggaaaactcg
     6961 gcgcctcgct acctcgacgg cgattctctc gctcgtcttg cttgccagca cggcttgggg
     7021 aacgcgcttc gctgcgcgcg cagacgagcc ttacgcgccc agccgcgact acgacttaca
     7081 ggatattcgg atccacttgt ggttggacgg cactggcccc cagtttcgcg gcgaagtaac
     7141 tgaacgcgtg gccgctcttc ggaatggcat tggcgaattc aagtttgatt ccgttggctt
     7201 gaatatccag gacgttaccg ttgatggggc ttcatcaaaa ttttcgacga ctcccacgca
     7261 gctgattgtc gcgcttgccc atcccaccac ccgcggcgag cggcatgaaa tcctaattcg
     7321 ctactctggt gcgcccaagc gcggactcta tttcatcctc ccgaatcaag attatccgca
     7381 tcagcccgcg gaagtttgga cccagggcga agcggaggac acgcgctact acattcccct
     7441 ttacgattat cccaacgacc gcgcgacgtc tgaaatgctg ctcacggttc catccagttg
     7501 gattaccgtt tccaacggcc aattgctggg cgtgaagaat gaagccggcg gcgcaaaaac
     7561 gtgggattgg aaacagtcgc agccgctttc aacctatctg atttccgcga tcgcgggcga
     7621 attcgacgag catgacgatt cctggaacgg cgtcgcgctg cgattcctgg tgccacgcgg
     7681 caaccaatcc gaaattccat cgacgtttgc gcgcaccaaa cagatgctcg atcttttttc
     7741 aagcaagctc ggcgtggctt atccctgggg gcagtacgcg cagacttcag tggacggctt
     7801 caccgaagga gggatggaaa ataccagcgc aaccacgttg agtgtccgcg atctggtgaa
     7861 tccgaagctg ctgccggaac tgcgtatcgg cgacgacttc gtgatttcgc acgaaatggc
     7921 acatcaatgg ttcggcgatt tggtcacctg caaggactgg gcgaatcttt ggctgaacga
     7981 aggattcgcc actttcttc
//
LOCUS       DATNDT010000342         5386 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2653444, whole genome shotgun sequence.
ACCESSION   DATNDT010000342 DATNDT010000000
VERSION     DATNDT010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5386
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2653444"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="VN788_11065"
     CDS             complement(<1..1091)
                     /locus_tag="VN788_11065"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HXU21112.1"
                     /translation="MEFSFRRIVWIVLDSVGIGEMPDAAAYGDSGSDTLGNIARRRVL
                     HLPELQRLGLANIKQLAHLDPVPDPAAAYGRCALASPGKDTTTGHWEMVGIHLEKPFP
                     LYPHGFPREVMDEFERRIGRSTLGNKPASGTEIIKELGEEHMHTGSPIVYTSADSVFQ
                     VAAHEDTIPLWELYKICETARAILRGPHEVGRVIARPFEGEPGSFKRTPDRKDFAVPP
                     PRGMLLDQLDAANVPVWSVGKIFDVFLGRGIRAYEKTTGNADGMAKTLGALPDFERGL
                     LFVNLVDFDQQYGHRNDVEGYAGALEEVDRWLPLLISRLSDTDLLILTADHGCDPTTP
                     STDHSREYVPLLAYRKRNQHGVNLGTRSTL"
     gene            complement(1081..2394)
                     /locus_tag="VN788_11070"
     CDS             complement(1081..2394)
                     /locus_tag="VN788_11070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXU21113.1"
                     /translation="MILRFFLALVLVVPLARPQTSQPSPNASQISDAEQKSLRSALER
                     TGGSPIELIHALEQHLAQYPTSPKRPELERILVKAAIESNDANRTILYGERVLAREPE
                     NVQVLERVSTALLEKPDHDRATRALDYAQRIDKALSTLGNTAHASGAEQARFIDQVDR
                     AAARGLLLQAKATDFLGDSGKATQLVVASYERYPSAEAAHEAAGLFEKAGKTDEAIRY
                     LTEAFTIADPHSTSADRARERAHMGELYRKTHGGSETGLGDLVLQAYDRTTAMSQERR
                     IKLRQYDPNADTKNPLDFTLSSVDGTKLNLAAMKGKVVVMDFWATWCGPCRVQHPLYQ
                     QLEQKFHNRADVVFLSISTDEDRELVKPFLRDQKWEQNVYFDDGLSIALHVSSIPTTI
                     VFGKTGELVSRLNGFDPSSFVSVVTERIEEALKAPAKAQEARNGV"
     gene            complement(2394..3023)
                     /locus_tag="VN788_11075"
     CDS             complement(2394..3023)
                     /locus_tag="VN788_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21114.1"
                     /translation="MKFLLVLALATGSLLAEGPRITYSKFFKGSQPEFVLITLDKNGA
                     AIYREAKDDPNPLEFHLTTSQAAEIFALAAKLDLFKRPLESGLKVANMGMKTFQYEDG
                     TVKNEVKFNYSLDLDARSLQDWFERMAETEGNYIDLDRTAHFDKLGVNDALLQLQETY
                     EHKRLVAPSQFIPLLQRIVKNESYLHMARERAAQLIEAFGGQPAKVAVQ"
     gene            complement(3020..3517)
                     /gene="ruvC"
                     /locus_tag="VN788_11080"
     CDS             complement(3020..3517)
                     /gene="ruvC"
                     /locus_tag="VN788_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HXU21115.1"
                     /translation="MRVLGIDCGAERTGYGLIESDGRAHRLVAAGVVRTHRSHPFPQR
                     LRQIAEGLRAVMEEYAPQVAAVEQVFHAQNVKTALILSHARGVALLTLAERGLEIGEY
                     SPLEVKMSVVGYGRAEKQQVQLMIHSILGLTEKIESEDACDAVAVAICHATRTASQIR
                     MGVTR"
     gene            complement(3531..4274)
                     /locus_tag="VN788_11085"
     CDS             complement(3531..4274)
                     /locus_tag="VN788_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXU21116.1"
                     /translation="MSGHSKWATIKHKKAALDAKRGKAFTRLIKEIMIAARSGGDPDM
                     NPRLRTAITAAKAVSMPADNIKRAVMRGTGELEGGQIDEIMFEGYGPGGAAVLVMVAT
                     DNRNRTVSEIRHAFSKNGGNLGEQGSVAWMFERKSQILIEKDKADEDTLMSLVLDAGA
                     EDLRGDEDNWEVVSAPENHDAVLEVLQKAGLPAASAEIAMLPKNLLKLEGKNAQAMLR
                     LSESLEEHEDVQNVYSNFDVDEQELEALA"
     gene            complement(4376..4777)
                     /locus_tag="VN788_11090"
     CDS             complement(4376..4777)
                     /locus_tag="VN788_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21117.1"
                     /translation="MLPGAAYLDDDEFLAAFYSFRLAPSEFHHADHLRLAWLEVHRQP
                     IEAVLSRVRAGIQAFAAHHDASHIYHETITTAWVRLIATHDEKSFDEFLNRNEHRLNL
                     DLLHRFWTPALLRSDEARARWMPPNLRSLPI"
     gene            complement(4779..>5386)
                     /locus_tag="VN788_11095"
     CDS             complement(4779..>5386)
                     /locus_tag="VN788_11095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21118.1"
                     /translation="YTLRFVARENGEGKVGTFETAFTIPDLSSGNSLRLSSVILSNQH
                     QALSQQIASVKNNKKLLADNPLIDSAGNKTVPNVTRVFHPNQDLYAYLEVYDPASPEN
                     LQSDIKIAGVTANLALYSGNRKVLETQPIRASRLEAKRDSALPLRLQTPLSSLKPGEY
                     TCQINVIDEFGHKFAFPRATIAVLPAATTVASAQQANQPGN"
BASE COUNT         1047 a         1580 c         1591 g         1168 t
ORIGIN      
        1 agcgtggagc gcgtgccgag attcacgccg tgctggttgc gcttccggta tgcgagcaga
       61 ggaacgtatt cgcggctgtg atcggtggat ggcgtagtcg gatcgcaccc gtggtcggcc
      121 gtgagaatca gcaggtcggt atccgacagt ctcgatataa gaagtggcag ccagcggtcg
      181 acctcttcga gcgctcccgc gtagccctcc acgtcattgc gatggccgta ctgctgatcg
      241 aaatcaacca gattgacgaa caacaggccg cgctcgaaat caggtagcgc tcccagcgtc
      301 ttcgccattc catccgcgtt gccggtggtc ttttcgtatg cgcgaatgcc gcggccgagg
      361 aacacgtcaa agattttgcc aacgctccat acgggaacat tcgctgcgtc cagttgatcg
      421 agtaacatgc cgcgcggcgg cggtacggcg aaatccttac ggtccggcgt gcgcttaaaa
      481 ctacccggct cgccctcaaa cggccgggca atcacgcggc cgacctcgtg cgggccgcgc
      541 agaatcgcgc gcgccgtttc acagatcttg taaagctccc acagcgggat tgtgtcctcg
      601 tgcgcggcga cctgaaacac gctatcggcg gacgtgtaga cgatgggcga gccggtgtgc
      661 atgtgctctt cgccgagctc tttgataatt tcggttcccg aggcgggctt gttgccgagc
      721 gtgctccggc cgatgcggcg ctcaaactca tccatcactt cgcgcgggaa cccgtgggga
      781 taaaggggaa acggtttttc gaggtggatg ccgaccatct cccagtgacc ggtagtcgta
      841 tctttgccgg gcgacgcgag cgcgcagcgg ccatacgcgg cggccggatc gggaaccgga
      901 tcgagatgcg cgagctgctt gatattcgct aggccgaggc gttgcaattc cggcaggtga
      961 agcacgcgcc ggcgcgcgat attgcccaga gtgtcgctac ctgaatcgcc ataggcggcc
     1021 gcatccggca tctcgccgat tcccacgctg tccagcacga tccagacgat acgccggaag
     1081 ctaaactcca ttgcgcgcct cttgcgcttt cgccggcgcc ttcaacgcct cttcgatgcg
     1141 ctcggttaca accgaaacaa aactgctggg atcgaagccg ttcaaccggc tcaccagttc
     1201 tccagttttt ccaaaaacga tggtcgtcgg aatcgaggat acatgcagcg cgattgacag
     1261 accatcgtcg aagtagacgt tctgctccca cttttggtcc cgcagaaacg gctttacgag
     1321 ttcgcggtcc tcgtcagtgc tgatcgagag aaacacaaca tcggcgcgat tgtgaaactt
     1381 ctgttccaac tgctggtaaa gcggatgctg cacgcggcag gggccgcacc aagtggccca
     1441 gaaatccatc acgacgactt tgcctttcat cgcggcgaga ttcagtttcg tgccgtcgac
     1501 ggaggacagc gtgaaatcga gcgggttctt cgtatccgca ttcgggtcgt actgccgaag
     1561 tttgatccgg cgctcttgcg acatggctgt cgtgcggtcg tatgcctgca gtacaagatc
     1621 gccgaggccg gtttccgaac cgccgtgagt tttccggtaa agctcgccca tgtgcgcgcg
     1681 ctcgcgggcg cggtccgcgc tggtggaatg cggatcggcg atggtgaagg cttcggtcag
     1741 atagcggatc gcctcgtccg tcttgccggc cttctcaaaa aggccagccg cctcatgcgc
     1801 ggcttccgcg ctgggatagc gttcataact cgcaaccacc aactgggtcg cttttccgct
     1861 atcgcctagg aagtccgtcg ctttggcctg cagaagcaac ccgcgcgcgg cggcgcggtc
     1921 tacctggtcg ataaaacgcg cctgctcggc tccgctcgcg tgggcggtat tgccgagagt
     1981 cgaaagcgct ttgtcgattc gttgggcata atcgagcgcg cgcgttgcgc gatcgtgatc
     2041 gggcttttcg agcagagcgg tcgagacgcg ctccagcact tgcacgtttt ccggttcgcg
     2101 cgcgagaaca cgctcgccat agagaatcgt acggttcgcg tcgttcgatt cgatcgcggc
     2161 tttcacgagg atgcgctcaa gctcgggacg cttcggagaa gtggggtatt gcgcgagatg
     2221 ctgctccagc gcgtgaatca gctcgatggg gcttccaccg gtccgctcga gcgcggagcg
     2281 caggctcttc tgttctgcgt cggagatctg gcttgcattc ggggatggct gactggtctg
     2341 cgggcgcgca agcggaacaa ccagaacgag cgccagaaaa aagcgcaaaa tcatcactgc
     2401 acagccacct tcgccggctg gccgccgaag gcttcgatca attgcgcggc gcgctcgcga
     2461 gccatgtgca gatagctttc attcttcaca atgcgctgca gcaagggaat gaattgcgag
     2521 ggcgcgacca ggcgcttgtg ctcataggtc tcctgcaatt gcagcaacgc atcgttcacg
     2581 ccgagtttgt cgaaatgcgc cgtgcggtcg agatctatat aattaccctc ggtttccgcc
     2641 atacgctcaa accaatcctg tagcgaccgc gcatcgaggt ccaaggaata gttgaacttc
     2701 acttcattct tcaccgtgcc gtcttcgtac tggaacgtct tcatgcccat attcgcgacc
     2761 ttcaggccgg attccagcgg acgcttaaac agatccagct tcgccgcgag cgcgaagatt
     2821 tcagcggcct gagacgttgt gagatggaat tccagcggat tcggatcgtc cttcgcctcg
     2881 cggtagatgg cggcgccgtt tttatcgagc gtgatcaaca caaactccgg ctggctgcct
     2941 ttgaaaaatt tcgagtaagt gatccgaggg ccctcggcca gcaggcttcc ggtcgcaagc
     3001 gccagaacga gaaggaactt catcgcgtca ctcccattct gatttgcgat gcggtacgcg
     3061 tcgcgtgaca gatcgccacc gccacggcat cgcaagcgtc ctcggactcg atcttctccg
     3121 tgaggccgag aatggaatgg atcatcagtt gcacctgttg cttctccgcg cggccgtaac
     3181 cgaccacgct cattttcact tcgagcggcg agtattcgcc gatttcgagg ccgcgttcgg
     3241 cgagcgtgag cagggccacc ccgcgggcgt gcgagaggat cagcgcggtc ttcacgtttt
     3301 gcgcatggaa cacctgctcg accgccgcga cctggggcgc atactcttcc attacggctc
     3361 gcaagccctc ggcgatctgc cgcaagcgct gcggaaaggg atgggagcga tgcgtcctga
     3421 cgactcccgc tgcaaccagc cggtgcgcgc gcccatcgct ttcgatcagg ccataaccgg
     3481 tccgctcggc gccgcaatca attccgagga cgcgcatcgc gagcgccgct ttaagcgagc
     3541 gcttccaact cttgctcgtc cacgtcgaag ttcgaataga cgttctgcac gtcctcatgt
     3601 tcttcgagcg actcgctcag ccggagcatc gcttgagcat tcttgccttc gagcttcagc
     3661 agattcttcg gaagcatcgc gatttcggcg gaagcggcgg gaaggcccgc tttctgaagc
     3721 acctcaagaa cggcatcgtg attctcggga gccgaaacca cctcccaatt atcttcgtcg
     3781 cctcgcagat cctcggcacc cgcgtcgagc acgagactca tcagcgtgtc ttcgtcggct
     3841 ttgtcctttt caatcaggat ctggcttttg cgctcaaaca tccaggcaac gctgccctgc
     3901 tcaccgagat tgccgccgtt tttcgagaag gcgtggcgta tttcactgac cgtgcgattg
     3961 cggttgtcgg ttgcgaccat cacgagcact gccgcgccgc caggaccata gccttcaaac
     4021 atgatttcgt cgatctggcc gccctcgagt tcgccggtgc cgcgcatgac cgcgcgcttg
     4081 atattatcgg ccggcatact gaccgctttc gcggcagtga ttgccgtgcg aaggcggggg
     4141 ttcatgtcgg gatcgccgcc gctgcgcgcg gcgatcatga tttctttaat aaggcgggtg
     4201 aacgctttcc cgcgcttcgc gtcgagagcg gccttcttgt gcttgatagt cgcccatttt
     4261 gaatgtcctg acatcggata cctcttgctc gggtgagcag tatttgaacg ctcgggtgag
     4321 cttaaacaac gaggatacca gaacgattac aacctgggca tcccgacacc gcaagctata
     4381 taggtaaact ccgcagattg ggcggcatcc atcttgccct cgcttcgtcg cttcgaagca
     4441 atgccggcgt ccagaaacga tgaagcaggt ccaggtttaa gcgatgttcg ttcctattca
     4501 agaattcgtc gaagcttttc tcgtcatgag tggcaatcag acgaacccaa gctgttgtga
     4561 tggtctcgtg ataaatatgg gacgcgtcat gatgcgcggc gaaagcctga atacccgcgc
     4621 gcaccctgga cagcaccgcc tcgatcggct gcctgtggac ctctaaccat gccaacctca
     4681 ggtgatcggc atgatggaat tcggagggcg ctagccggaa ggaatagaac gccgccagga
     4741 actcatcgtc atcgagataa gccgcgcccg gtagcattct aatttcctgg ttgattggct
     4801 tgttgggcgc ttgcgacagt agtggccgcc ggaaggacgg ctatggtcgc tcgtggaaag
     4861 gcgaacttgt ggccgaattc atcgatcaca ttgatctgac aggtgtactc gcccggtttg
     4921 agagagctta aaggcgtttg caggcgaagc ggcaacgcgc tatcccgctt ggcctccaaa
     4981 cggctcgcgc ggatgggctg tgtctcgagt accttgcggt tacctgagta cagggcgaga
     5041 ttggcggtaa caccggcaat cttgatatcc gattgtaagt tctcggggga tgctggatca
     5101 tatacctcaa gataagcgta aagatcctga ttcggatgaa acacgcgggt gacgttcggc
     5161 acggtcttat taccggcgct atcgatcaac ggattatcgg caagcagttt tttgttgttt
     5221 ttcacggagg cgatctgttg cgaaagcgct tggtgttgat tacttaggat cacggagcta
     5281 agccgaagcg aatttccgga agaaagatcg gggatcgtga acgccgtctc gaatgtgccg
     5341 acttttccct cgccattctc gcgggcaacg aaacgcagcg tatatt
//
LOCUS       DATNDT010000343         4987 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3812247, whole genome shotgun sequence.
ACCESSION   DATNDT010000343 DATNDT010000000
VERSION     DATNDT010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4987)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4987)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4987
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3812247"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1600
                     /gene="katG"
                     /locus_tag="VN788_11100"
     CDS             <1..1600
                     /gene="katG"
                     /locus_tag="VN788_11100"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HXU21119.1"
                     /translation="DLQNPLGAVQMGLIYVNPEGPNGKPDPIAAGRDIRETFRRMAMN
                     DEETVALIAGGHTFGKTHGAAVPADYVGAEPEGADIENQGLGWKNKFGKGFGADTIGS
                     GLEVTWTQTPTKWSNNFFKNLFEFEWELTKSPAGANQWRPKNGGGAGTVPDAHDPSKK
                     HAPTMLTTDLALRFDPAFGKISRRFYENPDQFADVFARAWFKLTHRDMGPIARYLGPL
                     VPKETLAWQDPIPAVDHVLINEQEVAALKAKILASGLTVSQLVSTAWASASTFRGSDK
                     RGGANGARVRLSPQKFWEVNQPTELAKVLEKLEAIQKEFNASASGGKKISLADLIVLG
                     GDAAIEKAARDAGTDVKIPFRPGRMDASQEQTDVESFEPLEPVADGFRNYRRSKSRLS
                     AEEQLVDKAQLLKLTAPEMTVLVGGLRVLGANAGNSKYGVFTKKPGTLSNDFFINLLD
                     MGTKWERSTSSNGSGDIYEGRDRKTNEVKWTGTRIDLIFGAHSQLRALAEVYASSDAK
                     QKFAKDFAAAWSKVMNADRFEAAS"
     gene            complement(1713..3677)
                     /locus_tag="VN788_11105"
     CDS             complement(1713..3677)
                     /locus_tag="VN788_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2075 domain-containing protein"
                     /protein_id="HXU21120.1"
                     /translation="MPVRTYYHAPISSFLQDDAERILGVLASQHHHALEESQRWAWLQ
                     QIAILKSSLVNQTAGRIFLEFFIPRMGKRADAIVVAKNIVFVIEFKTGANSYTSAAFQ
                     QVEDYALDLKNFHEGSHDAPIIPLLVSTDAESKEMSKLAFADDLVALPIGANSTNLGP
                     LIDGLCVGQSFPDIDIAKWMAKGYRPTPTIVQAAEVLYRTHSVLDIARSDASAQNLQE
                     TNSSVNAVINRARANHIKAICFVTGVPGSGKTLAGLNIATQRSVEHLDEHAVFLSGNG
                     PLVDVLREALTRDKAKRESIPKKRAEREVRSFIQNIHHFRDEYVGNTTIPLEKVAVFD
                     EAQRAWTRKQAAKFMRQKRGLSDFDMSEPEFLISVMDRHSNWCTIVCLIGGGQEIHTG
                     EAGISEWINALEGRFPKWEIHASSRMLLPEYGAAGLVERFAKSPRVYSDEYLHLAVSM
                     RSFRAEALSEVVGHIVDNEPKAASAAYKKIASTYPIFLTRNLSIAKAWLRSRARGTER
                     FGLVASSGALRLRAEGIHIKAELEPANWFLNDSSDVRSSNYLEDVASEFAVQGLELDW
                     VGVCWDGDFQHKDDGWIYKAFKGTKWQSISDESRRKYLKNAYRVILTRARQGMVLFVP
                     LGEPSDHTRLPSFYDETFAFLHSCGIPSLD"
     gene            complement(3711..4367)
                     /locus_tag="VN788_11110"
     CDS             complement(3711..4367)
                     /locus_tag="VN788_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HXU21121.1"
                     /translation="MISAISFIIPDIVISDKTQSISPNDASPEQIWNSLVEGNQRFVA
                     GKPMARDIVSQRRSLEKTQNPPVSVLSCSDSRLPSEAIFDQGLGDLFVVRIAGNSAEP
                     MGIGSLEFAVRILGTKVIVVLGHQRCGGVTAACSEETMASSNLEALIEPIKESCRMAK
                     EQHGSEDLIDSAIKIHIRKTVNDLLAQSEILKHAHDAGKLSIIGAYYSLDSGVVTRLR
                     "
     gene            4441..4911
                     /locus_tag="VN788_11115"
     CDS             4441..4911
                     /locus_tag="VN788_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXU21122.1"
                     /translation="MDNIQIRPAHASDLSQLVSLCEALWPETSAEEHARDLRLILEGN
                     VAAVLTTPIIIFVAVAGDANLVSFVEVDIRSHADGCDPLHPAGYIEGWYVAAAYRHLG
                     VGKRLLAQAEDWARSHGCLEIASDALIDNSLSQRAHEALGYEVVDRCVHYRKRL"
BASE COUNT         1163 a         1283 c         1377 g         1164 t
ORIGIN      
        1 cgatctgcaa aatccgctgg gcgcagtcca gatggggctg atttacgtga atcccgaagg
       61 tccgaatggc aagcccgatc cgattgcggc gggcagggac attcgcgaaa cgttccggcg
      121 catggccatg aacgacgaag agacagtcgc cctgattgct ggcgggcaca ccttcggcaa
      181 aactcatggc gctgccgtgc cagccgacta cgtgggagcg gagccggaag gcgctgacat
      241 tgagaaccag ggccttggct ggaaaaataa atttggcaag ggctttggcg cggacaccat
      301 cggcagcggg ctggaagtga cctggacgca gacgcctacg aagtggagca acaacttctt
      361 caagaacctc tttgagttcg aatgggagct gacgaagagt ccagccggag cgaaccagtg
      421 gaggcccaag aatggcggcg gggcaggcac ggtgccggac gcgcatgatc cgtcaaaaaa
      481 acacgctccc accatgctga ccacggacct tgcgctgcgc tttgaccctg cgttcgggaa
      541 aatttcgcgg cggttttatg aaaatcccga tcaattcgcg gatgttttcg cgcgcgcatg
      601 gttcaaactt acgcatcgcg acatggggcc gattgcccgc tatctgggcc cgctggttcc
      661 gaaagaaacg ttggcgtggc aagacccgat tcccgcggtg gaccatgtgc tcatcaacga
      721 gcaggaggtt gctgcgctga aggccaagat tctggcttcg ggattgacgg tttctcaact
      781 ggtctcgact gcgtgggcgt cggcgtcaac cttccgcgga tcggacaagc gcggcggcgc
      841 gaacggcgcg cgtgttcggc tctcgccgca aaagttctgg gaggtcaacc agccgacgga
      901 attggcgaag gtgctggaaa aactggaggc gatccagaaa gaatttaacg cctctgcatc
      961 cggcggaaag aaaatttcgc tggcggacct gattgtgctt ggcggcgacg ctgccatcga
     1021 gaaagccgcc agggacgcgg ggaccgacgt gaagattccc ttcaggccgg ggcgcatgga
     1081 tgcatcgcaa gagcaaactg atgttgagtc cttcgaaccg ctcgaaccgg tggcggatgg
     1141 tttccgcaac tatcgccgca gcaagtcgcg tttgtcggct gaagagcaat tggttgataa
     1201 agcacaattg ctaaagctga ctgcgccgga gatgacggtg cttgtcggcg gcctgcgcgt
     1261 tcttggagcg aatgccggaa attcaaaata cggcgtcttc accaaaaagc cgggcacgct
     1321 cagcaatgac ttcttcatta acttgctcga tatgggcacg aagtgggagc ggtcaaccag
     1381 ttccaacggc tccggcgaca tttacgaagg ccgcgaccgc aagacaaacg aagtgaagtg
     1441 gacgggaacg cgcattgacc tgatcttcgg tgcgcactcg cagttgcggg cgctggcaga
     1501 agtctatgct tcgtcggacg cgaaacagaa attcgcgaaa gactttgcgg cggcttggag
     1561 caaggttatg aatgcggata ggtttgaggc ggcgtcgtaa gtctcggtaa ggattggcgt
     1621 cgtcaaccgg tacgacttgt cgcagaaatc acaaactcgc agctcgacaa tggctccgac
     1681 aattcggagc cattgtcgtt aagtggccac gcctaatcga ggctagggat tccgcaggag
     1741 tgaagaaaag cgaacgtttc gtcgtaaaaa gatgggagac gcgtatggtc agatggctct
     1801 ccgagtggaa cgaagaggac catcccctgt cgcgctcgtg tcaaaattac tcggtaggca
     1861 ttcttcagat acttccgcct tgattcgtca ctgatagatt gccactttgt tcctttgaaa
     1921 gccttgtata tccaaccatc atccttgtgt tgaaaatctc catcccaaca cacgcccacc
     1981 caatcaagtt cgagaccctg aacggcaaat tcgcttgcca catcttctag atagttcgac
     2041 gatcttacat ctgagctatc gtttagaaac caattcgcag gttccagctc agctttaatg
     2101 tgaattcctt ccgcccgaag acgtagtgct cccgaagatg cgactaaccc gaagcgctct
     2161 gttccacgag cgcggctgcg tagccaagcc tttgcgatac taagattgcg agtcaagaag
     2221 ataggatagg tggatgcaat ctttttataa gcagcactag ctgcttttgg ttcgttgtcc
     2281 actatgtgtc cgaccacttc tgaaagtgct tcggctcgaa atgaacgcat cgagactgca
     2341 agatggagat attcatctga gtaaactcgt ggagactttg caaatcgttc tactagacca
     2401 gcagcgccat attccggaag taacatgcgc gacgacgcat gaatttccca tttaggaaaa
     2461 cggccctcta gagcatttat ccattcagaa atccctgctt cgccagtgtg aatctcttgc
     2521 cctccaccga tgaggcaaac gatcgtacac caattagagt gtcggtccat gacgcttatg
     2581 aggaactctg gctcagacat gtcaaagtca gatagcccac gtttttggcg cataaacttc
     2641 gccgcctgct tgcgcgtcca ggcgcgttga gcttcgtcaa acactgcgac cttctcgaga
     2701 ggaatggtcg tatttccaac gtactcatcc cgaaagtgat ggatattctg aataaaactt
     2761 cgaacttcgc gttccgctcg ctttttagga atagattcgc gcttagcttt gtcacgcgtg
     2821 agagcctccc taagaacgtc aaccaggggg ccgttacctg aaaggaagac ggcatgttca
     2881 tcgagatgct caaccgatcg ctgcgtggca atattcaagc ctgcgagcgt ttttcctgat
     2941 ccaggtacac cagtcacgaa gcagattgct tttatgtgat ttgctcgagc acgatttata
     3001 actgcattaa cacttgagtt cgtttcttgg agattttgtg cacttgcgtc tgatctcgca
     3061 atgtcgagta cgctgtgtgt tcgataaagc acttcggccg cctgaacaat ggtcggtgtt
     3121 ggccgatatc ctttcgccat ccattttgcg atgtcgatat ccgggaagga ctgacccacg
     3181 caaagaccgt caattagtgg tcccaaattc gtactgtttg ctccaatggg caaagctaca
     3241 aggtcatcgg caaatgccaa cttggacatt tctttggact ccgcatcggt tgagactaac
     3301 aaagggatga ttggagcgtc gtggctgcct tcgtgaaaat tctttaggtc gagagcgtaa
     3361 tcttccactt gttgaaacgc agctgacgtg tatgagttcg cgccagtctt aaattcgatt
     3421 acaaatacga tatttttcgc aacgacaatt gcatcagccc gcttacccat tctaggaata
     3481 aagaattcta aaaagattct cccagccgtt tgattgacga gtgaactttt gagaatcgca
     3541 atttgttgaa gccatgccca gcgctgcgac tcctccaggg cgtgatggtg ctgtgacgcc
     3601 aaaactccga gaatacgttc ggcatcgtct tggaggaagc tacttattgg tgcatgatag
     3661 taagtccgaa ctggcataga aggccattaa gttcacgacg gcgattccgt ttaccgcagc
     3721 cgcgtgacca cacctgaatc gagcgaataa tatgccccaa taatgctcag cttccccgcg
     3781 tcatgagcat gcttaaggat ttcactctga gcgagcagat cgttcacggt cttgcgtatg
     3841 tgaattttta ttgcggaatc aatcaggtct tctgatccgt gttgttcttt tgccatcctg
     3901 catgactcct tgatcggctc aatcaaagct tccaggttcg aggatgccat tgtctcttca
     3961 gagcaggcgg ccgtgactcc gccgcagcgt tgatgcccga gcactacgat cactttggtc
     4021 ccgagaatcc ggaccgcaaa ttcaaggctg ccgatgccca tgggttcggc gctattgcct
     4081 gcaatccgga ccacaaacag atcgccgagg ccctgatcga aaattgcttc tgatggaagg
     4141 cggctatcgg agcaactgag caccgaaact ggcgggttct gggtcttctc caggctgcgg
     4201 cgttgggaaa cgatgtcgcg agccatcggc ttgccggcga cgaaacgctg gttgccctct
     4261 actaaagagt tccaaatttg ttccggggaa gcgtcatttg gggagattga ttgtgttttg
     4321 tctgaaatca cgatgtctgg aatgataaat gagattgccg atatcatatt ggcaaagctc
     4381 gcggttgcga ccggggctaa tcaatcaatg tttccgcgat gaggcttccg caggctcgac
     4441 atggataata ttcagatcag acctgctcac gcgtcggacc tcagccaact ggtgtctctg
     4501 tgcgaggcgt tgtggcctga gacttccgcg gaagaacatg cccgggatct gcggcttatc
     4561 ctcgaaggaa acgttgctgc cgtactcacc acgccaatca tcatttttgt agcagtggcg
     4621 ggcgacgcga atttggttag ctttgtggaa gttgacatcc gttcgcatgc cgacggctgc
     4681 gacccgttgc atccagcagg ttacatcgag ggatggtatg tggctgcggc ctatcgccac
     4741 ctcggcgttg gcaagcgatt gctcgcccaa gcggaggatt gggcgcggag ccatgggtgc
     4801 ctggagatag cttccgatgc cttgattgac aactcgctct cccagcgcgc gcatgaggct
     4861 ttaggctacg aggtggtaga ccgctgtgtg cactaccgga agaggctttg aggaaatgca
     4921 ctggatactc tggagtttag ctgtcgtagt gctacaatgc tcccacttgt aagttactca
     4981 gtaaacg
//
LOCUS       DATNDT010000344        11317 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2303196, whole genome shotgun sequence.
ACCESSION   DATNDT010000344 DATNDT010000000
VERSION     DATNDT010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11317
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2303196"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..830
                     /locus_tag="VN788_11120"
     CDS             <1..830
                     /locus_tag="VN788_11120"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21123.1"
                     /translation="AQALATKESARPGSASGEPKMVWAVPCDCTSGDRTGRVLAKARV
                     PERYRHCDFENYETDNEIENTPKPQLDAWGRSLSQAKLIVQRFAQEFSPIPEMHSEHG
                     LLLMGPCGVGKTHLAVAALKEIVLRGHGGLFYDYRELLKEIQDSYNAESQSTEMSVLE
                     PVLKADILVLDDVGSSKPSLWALETVGHVLNTRYNEKRVTLLTTNFLDSEAAANGAAA
                     GAAPTRVAGMRTPMLDDSLTERVGKRIRSRLYEMCRTVEISAPDYRKEIRHAGRFRS"
     regulatory      933..1079
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            1150..2130
                     /gene="moaA"
                     /locus_tag="VN788_11125"
     CDS             1150..2130
                     /gene="moaA"
                     /locus_tag="VN788_11125"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HXU21124.1"
                     /translation="MLLADKFGRRITDLRVSVTDRCNFRCVYCRSANPETHMPARDLL
                     TWDEYERLVRILVGMGIRKVRVTGGEPLVRPGVEDFIGRLKALGVEDLSMTTNGYMLA
                     ERCDRLVAAGMDRINISLDSLDREKFERITRTKTFHQVIAGIDAAQASALRPVKVNAV
                     LVRGVNDDEVEAFAAFARDRDLIMRFIEFMPLDADRAWSRELFVPGEEVYRRISAQWP
                     LVPVAHERSETARKYRFADGRGEIGLISPVSQPFCGFCSRIRLTADGKLRTCLFSKDD
                     HDLRSLLRDGSTDREICARICDIVAQKEKGHRINEPGFLPPSRTMVSIGG"
     gene            2222..2674
                     /locus_tag="VN788_11130"
     CDS             2222..2674
                     /locus_tag="VN788_11130"
                     /inference="COORDINATES: protein motif:HMM:NF021486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2203 domain-containing protein"
                     /protein_id="HXU21125.1"
                     /translation="MAEEPNESKLFSLSEAERLRAQIEPVLIETIESRRKLAELDEEL
                     GGLRERIMRSGGMLVAYEKTAGRRLERNRLAEIIEAAIEQIHSTGCIVKDIEVGLLDF
                     PARMNGEDVYLCWRLGEDRIRFYHRQDEGFAGRKPIDPRDAGYSNPIQ"
     gene            2726..4852
                     /locus_tag="VN788_11135"
     CDS             2726..4852
                     /locus_tag="VN788_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXU21126.1"
                     /translation="MTQRAQNRLKDSASPYLRSAAHQPVDWHEWGEEAFQKARAESKP
                     ILLDIGAVWCHWCHVIDRESYENPEIAAAINQLYIPVKVDRDERPDVDARYQSAVSAI
                     SGQGGWPLTAFLTPDGKPFFGGTYFPPEDAGGRPGLKRILQAVSEAFKSRRSEVDNSA
                     QALEEAVAKAEIFQGARGVFDVRVVDAVIEAALRQFDERHGGFGDAPKFPHASTIDLL
                     LERFQATRNPQLLPVADRTLEGMALGGVYDQLAGGFHRYSVDERWCVPHFEKMSYDNS
                     ELLRNFLHGYQVTRKLLFRETAEGIIAWVNEVLSDQERGGFYASQDADQTLDDDGDYF
                     TWTVAEARPELASEEWRIVERYYDIAPQGEMHHNPEKNVLWVADNVENISRKLSIGSD
                     EAALLLARAKEKMLAARRRRTPVPAIDQTLYVGWNAMFISAYLDAANVLGRTDCRDFA
                     LKTLDRMLAEAWDESKGFLHRIGGPRLEGSLDDQVLAVAALLDAYELTLEPSYFHAAE
                     SAMHLALERYGDPEGGGFFDRAKDGAPMGGLEVRRKPLQDSPTPAGNSVAAIVLERLY
                     GFTGETLYHQWSAKTLEAFIGLIPRYGLFAGTYALAALLHARHPLQVVVTGAAGDPTA
                     GQLENAAREVYRFGKAVLRVTPERLASSNLPSALRETLPHLDATVPQALVCVEAVCYP
                     PLSDHEKLTALLMDSTFGTPEAAPQQ"
     gene            4930..5535
                     /gene="lepB"
                     /locus_tag="VN788_11140"
     CDS             4930..5535
                     /gene="lepB"
                     /locus_tag="VN788_11140"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HXU21127.1"
                     /translation="MDDASTPGAANSSDPLQQPPPLRRELRSWTRDLAVALGLAIVVM
                     IFLYQPVKVEGTSMNPLLSDQERIFINKFVYHFEPIERGDVIVFWYPLDRSKSFIKRV
                     VGLPGETIEIRDGHVYINGHELADQYVPANYLDGSTYPPHHIPADDYFVMGDHRDSSN
                     DSRVFGPVSRQFIYGKAVFAYWPADHFGSLTSTSTVNAASK"
     gene            5563..6858
                     /gene="carA"
                     /locus_tag="VN788_11145"
     CDS             5563..6858
                     /gene="carA"
                     /locus_tag="VN788_11145"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF009475.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HXU21128.1"
                     /translation="MNEALAGRATLALEDGRVFNGRAAGARTRRGGEVVFNTSLTGYQ
                     EVFTDPSYAGQIVCLTYPHIGNVGANLEDEESAKPWIEALIVREFSALASNWRSGESA
                     QQYLERYGVPVIWDVDTRALVRHLREVGALRGIISTDGTTAEQLIAQARALPTMAGQE
                     LASRVTVAKKYDWTRGSIDLAAPIASSAAAAAGDSSVRADRVNGKRGENGAAASKEAT
                     GSSGGRGFSSGIEPAKQSALAPEERYRVVAYDYGIKQNILRLLVDHGCDVTVVPAQTS
                     AADVLALKPHGVFLSNGPGDPEPILYAVENIRKLLGRVPIFGICLGHQLCGLALGGRT
                     FKLKFGHHGSNHPVKNLLTEKVEITAQNHGFCVDPDSLPSSDVEITHVNLNDHTNEGM
                     RHRSLPLFSVQYHPEASPGPHDARYLFDDFIAMMKAAAK"
     gene            7472..10726
                     /gene="carB"
                     /locus_tag="VN788_11150"
     CDS             7472..10726
                     /gene="carB"
                     /locus_tag="VN788_11150"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HXU21129.1"
                     /translation="MPKRTDLKKILIIGSGPIVIGQACEFDYSGTQACKALRAEGYEV
                     VLVNSNPATIMTDPEIADRTYIEPLAKEYLEAIIAAERPDALLPTVGGQTGLNLAVEL
                     AEAGILEKYGVKLIGAQLDAIKIAEDRLLFKDVCRSVGLEVPASAVVTNIPEAIDLAD
                     KLGYPVVIRPSFTLGGTGGSIAYNHEEFGDAIARALDASPVRQALIEESVIGWKEFEL
                     EVMRDFRDNFVVICSIENVDPMGIHTGDSITVAPAQTLTDKEYQRMRDAAAAIIRAVG
                     VETGGSNIQFAIDPETGRMVVIEMNPRVSRSSALASKATGFPIAKIAAKLAVGMTLDE
                     IPNDITRKTPSCFEPSIDYVVAKIPKWQFEKFPGSETTLTTQMRSVGEVMAIGRTFQE
                     SLCKAIRSLEPSTAWRAPAEVTTALLREKLHVPGPDRIHWLFEALERGMTRDEVCSLT
                     KIDPWFICQMEEMVALDRRVAASSLRGISRELLLESKRSGASDEELAKLWNVKSASVR
                     ARRHELGVLPVFKRVDTCAAEFESFTPYLYSTYEEEDESGAVDRPKIVILGSGPSRIG
                     QGIEFDYCCCHAALALKEDGYETVMINCNPETVSTDYDTSDRLYFEPLTLEDVLAVLE
                     REKPQGVIVQFGGQTPLNLALELKRNRAKIIGTDPESIDLAEDRRRFGALLAELNIPQ
                     PRGATALAPQEAVVTAAEIGLPVLVRPSYVLGGRAMVIAYDLKTVEEYVMHAAQIGGI
                     SDPKRPILIDQFLENATEVDVDALADGDDIVIAGIMEHIEEAGIHSGDSSCVLPPVTL
                     KPEVLAHIRDYTSRLAKALHVVGLMNVQYAIQHDTVYVLEVNPRASRTVPYVSKATGV
                     PLAKVAARLMTGRKLKDLRLPVIQTNGIQELAVRDFYAVKSPVFPFNKFRGIDTILGP
                     EMRSTGEVMGISSTFGLAFAKAQLAAGQRLPLEGMVFISVNDHDKRHVASVVKDLAVA
                     GLKILATRGTATALTRAGIEVEAVFKVNEGRPNVVDLIKTGKIQLVINTPLGRESFYD
                     ERSIRRAAVRYNVPCITTLSAASAAARGIRAMAGHAVEVAALQDLHPRKSAALP"
     gene            complement(10791..11249)
                     /locus_tag="VN788_11155"
     CDS             complement(10791..11249)
                     /locus_tag="VN788_11155"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HXU21130.1"
                     /translation="MTQPPPKPKRWLLAVDPRVYHWDTLFVKGKEMWRHAGNRPDAAR
                     QLKQVHKGDRALCYHGKPEHSLYALAEVTRDPYPDPHDADAKKYVMDLRAIERLPRVV
                     RLAEMRENRLLRKIKFLKNTRLTICPLTEEEYAEILRMAGIVASPGIPLP"
BASE COUNT         2319 a         3241 c         3411 g         2346 t
ORIGIN      
        1 gcgcgcaggc gctggcgacg aaagaatcgg cgcggcccgg ttcggctagc ggcgaaccaa
       61 aaatggtttg ggcggtcccg tgcgattgca cgtcgggaga ccgcacgggg cgtgtgctag
      121 cgaaggcgcg cgtccccgaa cgatatcgcc actgcgattt cgagaactac gaaacagaca
      181 acgaaatcga gaatactccc aagccgcaac tggatgcgtg gggccgcagc ctgtcgcagg
      241 ccaagctgat cgtccagcgc ttcgcgcaag aattttctcc cattcccgag atgcacagcg
      301 aacacggctt gctgctgatg ggaccgtgcg gagtgggtaa gacacacctc gccgtcgcgg
      361 cgctcaagga aattgtgctt cgcggacacg gcgggctttt ctacgattac cgcgagctgc
      421 tcaaggaaat tcaggatagc tacaacgccg aaagccagtc cacggagatg agtgtcctcg
      481 aaccagtgct taaggccgat attctggtgc tggacgatgt ggggtcgagc aagccgtcgc
      541 tctgggccct ggaaaccgtg ggacacgtgc tgaacacgcg ctataacgag aagcgcgtta
      601 ctttgttgac gacaaatttt ttggattcgg aagcggcggc aaacggcgcg gccgccggcg
      661 ctgcgccgac ccgcgtggcg gggatgcgga ctccgatgct cgatgattca ttaactgagc
      721 gcgtgggcaa gcgcatccgt tcgcggctat atgaaatgtg ccgcacggtg gaaatttcgg
      781 cccccgatta tcgcaaagag attcgccacg cgggacgctt caggagctag ccgcagctcg
      841 gaagcgtatt tatgaatcct attatttctt attgaacagc atatattcgc ctaggctagg
      901 cgatttgcct catcgcctat aaacgagtag actccggttg agttctccga cctctcggac
      961 ctgacggaga gaaatcccat tggccgggag gcgacgctcc gcgaagcagc ggagctgagg
     1021 gtgcgggtgg aaacgcccgg gcctcccgtg cttggaaagg ggaatgtctg gcgcgcgtgt
     1081 tcgcgccgga tgtgtagccc aaatccaaac gcggatttgg gctttttgtt ttgcaggagc
     1141 aaaaaccgaa tgttgcttgc tgataaattc ggccgccgga tcaccgacct gcgcgtgtcg
     1201 gtcacagacc gctgcaattt ccgctgcgtg tattgccgct ccgcgaatcc ggaaacgcat
     1261 atgccggcgc gcgatttact tacgtgggac gagtacgagc gactggtccg tattctcgtc
     1321 ggcatgggca ttcgcaaggt tcgagtgact ggaggggagc cgctggtacg tcccggagtg
     1381 gaagatttca tcgggcgact gaaggcgctc ggcgtggaag acctttcgat gaccaccaac
     1441 ggctacatgt tggcggaacg ctgcgatcgg ctagtggccg cagggatgga tcggatcaat
     1501 atcagcctgg attcgctgga ccgtgaaaaa ttcgaacgga ttacgcgcac gaaaacattc
     1561 catcaggtga ttgccggaat cgatgcggcg caggcttcgg cgctgcgacc ggtaaaggtg
     1621 aatgcggtgc tggtgcgcgg cgtgaacgac gatgaggtgg aagcttttgc ggcgttcgca
     1681 cgcgatcgcg atttgatcat gcgattcatc gaatttatgc cgctggatgc ggatcgcgcg
     1741 tggtcacgcg aactgttcgt tcctggcgag gaagtctacc gacggatttc ggcgcaatgg
     1801 ccgttggtgc cggtggcgca tgaacgcagc gagacggcgc gcaagtaccg gttcgccgac
     1861 gggcgcggcg agatcggcct gatttctccg gtgtcgcagc ccttttgcgg attctgctcg
     1921 cgcatccgcc tgaccgccga cggcaaactg aggacctgcc tcttcagcaa ggacgatcac
     1981 gatttgagat cgctgctgcg agacggctcg accgaccgcg aaatttgcgc gcggatctgt
     2041 gacatcgtcg cgcaaaaaga aaaagggcat cgcatcaacg agccgggttt cctgcccccc
     2101 tcgcgcacga tggtatccat cggtgggtaa tgttgcgctc ttctgaccgt ttcccgcatc
     2161 tgaaatacta gccgtgacga atgagctttt cgtcacgcgt tttgtgttgg gctagaggcg
     2221 tatggctgag gaaccgaatg agtccaaatt gttttcgctc tccgaggcag agcggctgcg
     2281 cgcccaaatc gagcccgtgc tgatcgagac gattgaatcg cgccgaaagc tggcggagct
     2341 cgacgaggag cttggcggtc tgcgggagcg catcatgcgc tcgggcggga tgcttgttgc
     2401 ctatgaaaaa accgccgggc gccggctgga acgcaatcgg cttgcggaaa ttattgaggc
     2461 agccatcgag caaattcatt ccaccggatg catcgtgaag gatatcgagg taggtctgct
     2521 ggattttccc gcgcgcatga acggcgagga tgtttatctg tgctggcgcc tcggcgaaga
     2581 ccgcattcgt ttctaccatc gacaggacga gggcttcgcc ggccgcaagc caatcgatcc
     2641 gcgcgatgcc ggctacagca atccgatcca gtagctggtt gaggcttact ccgcaggctt
     2701 tagcattgtg tgataggttg ctgttgtgac gcaaagggcc cagaaccggc tcaaggattc
     2761 cgcgagccct tacctgcgtt cggccgcgca tcaacctgtt gactggcacg aatggggcga
     2821 ggaggcgttt caaaaagcgc gtgccgaatc gaaacccatc ctgctcgata tcggcgcggt
     2881 gtggtgccat tggtgccatg taattgatcg cgaaagctac gagaatccgg aaatcgcggc
     2941 ggccatcaat cagctttaca ttcccgtaaa ggtggatcgc gacgagcgtc ccgacgtgga
     3001 cgcgcgctac caatccgccg tgagcgcgat ttctggacag ggcggctggc cactcaccgc
     3061 ttttctcact ccggatggca agccgttttt cggcggtacc tattttccgc ccgaggacgc
     3121 cggcggcagg ccgggtctga agcgtatttt gcaggccgtt tccgaggctt tcaaaagccg
     3181 ccgcagtgaa gtggataatt ccgcgcaggc gctggaggaa gctgtcgcca aggcggagat
     3241 ttttcagggc gcgcgcggcg tatttgacgt ccgtgtggtg gatgcggtga tcgaagctgc
     3301 cttgcgtcag ttcgacgaaa ggcatggagg ctttggtgac gcgccgaagt ttcctcacgc
     3361 cagtaccatc gatttgctgc tcgaacgttt tcaagccacg cgaaatcctc aattgcttcc
     3421 cgtggcggat cggacgctcg aaggcatggc gctcggcggc gtgtacgacc aactcgccgg
     3481 cggatttcac cgctattcgg tggacgagcg ctggtgcgtc cctcacttcg aaaaaatgtc
     3541 ctatgacaat tcggaacttc tgcggaattt cctgcatggc tatcaggtca cccggaagtt
     3601 gcttttccgc gaaaccgccg aaggcatcat cgcctgggta aacgaagttc tgtccgatca
     3661 agagcgcgga gggttttacg caagtcagga tgcggaccaa actcttgacg atgacggcga
     3721 ctatttcacg tggacggtcg cggaggcccg ccccgaactc gcgtctgagg aatggcgaat
     3781 cgtcgagcgg tattacgata tcgctccgca gggggagatg caccacaacc cggagaaaaa
     3841 cgtcctgtgg gtggccgata atgtggagaa tatctcgcgc aagctgagca tcggttcaga
     3901 tgaggcagct ctacttctcg ctcgcgcgaa agaaaagatg ctcgccgcgc gccgccggcg
     3961 tacacccgtt ccggccattg atcagacgct ctatgtcggc tggaacgcga tgttcatctc
     4021 cgcatatctg gacgcggcga acgtgctcgg gcggaccgat tgccgcgact tcgcgctgaa
     4081 gacgctcgat cgcatgctcg cggaagcgtg ggatgaatcg aaaggctttt tgcaccgcat
     4141 cggagggccg cgcctggaag gttcgctgga tgatcaggtg cttgcggtgg ctgcgctgct
     4201 cgatgcctac gaattgacac tcgagccgag ctattttcac gcggccgaga gcgcaatgca
     4261 tctggccctc gaaagatacg gcgatcccga aggcggcgga tttttcgatc gcgctaagga
     4321 tggtgctccg atgggcgggc tcgaagtccg gcgcaagccg cttcaggatt cgcccactcc
     4381 cgccggaaat tcggtggcgg cgattgtcct cgaaaggctc tacggtttca cgggagaaac
     4441 tctgtatcat cagtggtccg cgaagaccct ggaggctttc atcgggctga tcccgcgcta
     4501 cggtcttttt gcgggaacct acgcactcgc cgcactgctt catgcgcgcc atccgctgca
     4561 ggttgttgtc accggcgcag ccggcgatcc aacagctggc caactcgaaa acgccgcgcg
     4621 cgaagtctac cgttttggaa aagcggtgtt gcgagtcact ccagagaggc ttgcatcgag
     4681 caaccttccc tccgcattgc gagaaaccct tcctcatctc gacgcaacgg tcccgcaagc
     4741 tctagtctgc gttgaggcgg tatgctatcc gcccctctcc gaccacgaaa agctgaccgc
     4801 actgctgatg gactccactt ttgggactcc cgaggcggca ccgcagcaat gaagggcatc
     4861 caagtcaacg tgcagccgcc ttctttgcca gtatttaggc aggcatgata taaataaatg
     4921 tattgagata tggacgacgc ttctactccc ggcgcagcca attcctctga cccgttacag
     4981 cagccgcctc ccttgcggcg tgagttacgc agttggacgc gcgacctcgc agtcgctctg
     5041 ggcctcgcga tcgtggtgat gattttcctt taccagccgg taaaagtgga aggcaccagc
     5101 atgaatccgt tgctgagcga tcaggaacgc attttcatca acaaattcgt ttatcacttc
     5161 gagccgatcg agcgcggtga cgtgatcgtt ttctggtatc ctttggaccg ctcgaaatcg
     5221 tttatcaagc gggtggtcgg gcttccgggt gaaaccatcg agattcgcga cggccatgtt
     5281 tacatcaacg gccacgaact ggccgatcag tacgttcccg cgaactattt ggacggatcc
     5341 acgtacccac ctcaccacat tccggcagac gattactttg taatgggcga tcatcgcgat
     5401 agttccaatg actcgcgcgt ttttggtcca gtgtcacggc aattcattta tggcaaggct
     5461 gtattcgctt actggccggc tgaccacttc ggttcgctga cttctacttc gacggtcaac
     5521 gccgcatcga aatgacattc ttcaaaactt ctggaggaca cactgaacga ggccctcgct
     5581 ggacgcgcga cgctcgcgct cgaggacgga cgagttttta acggacgcgc ggcgggcgct
     5641 cgcacgcgcc gcgggggtga agtcgttttc aacaccagcc tcaccggcta ccaggaagtt
     5701 ttcaccgatc ccagctacgc cggccagatc gtctgcctca cttatcctca tataggaaat
     5761 gttggcgcga atctggaaga cgaggaatcc gcaaagccct ggatcgaagc gctgatcgtg
     5821 cgggaatttt ccgcgctggc ttccaattgg cgttcgggcg aatccgcgca gcaatatctt
     5881 gaacggtacg gcgtgccggt aatttgggat gtcgatacgc gcgcgctggt gaggcatctc
     5941 cgcgaagtgg gcgcgttgcg cgggattatt tccacggacg gcacaaccgc cgaacagctt
     6001 atcgcccagg ctcgcgcgct tcccaccatg gctggccagg aactcgccag ccgcgtcact
     6061 gtggcaaaga aatatgattg gacgcgcggc tcgatcgatc tcgccgcgcc aatcgcgagc
     6121 tctgccgctg ctgcggctgg ggactcctcg gtgcgcgccg accgggtaaa cggtaaacgc
     6181 ggcgaaaatg gcgccgccgc ttcgaaagaa gccacaggca gttcaggggg tcggggcttt
     6241 agctccggca tagagccagc taagcaaagc gctttagccc ctgaagaacg ttatcgcgtc
     6301 gtcgcctacg attacggcat caagcaaaat attctgcggc tgctggtcga tcacggctgc
     6361 gacgtgaccg tggtgccggc gcaaacttcc gccgccgacg tgctggcgct gaagccgcac
     6421 ggcgttttcc tttcaaatgg gccgggcgac cctgagccga ttctttacgc cgtcgaaaat
     6481 attcgaaaac ttcttgggcg cgttccaatt ttcggcattt gcctggggca tcagctgtgc
     6541 ggactggcgc tcggcggccg caccttcaaa ctaaaatttg gacatcacgg ctcgaaccat
     6601 ccggtgaaaa acctgctgac ggagaaagtg gagattaccg cgcagaacca cggcttctgc
     6661 gtcgatccgg attcgttgcc atccagtgac gtcgagatca cgcacgtaaa tttgaacgat
     6721 cacaccaacg agggcatgcg tcaccgctcg ctcccgctct tcagcgtcca atatcatcct
     6781 gaggcgtctc cgggtcccca cgatgcgcgt tatctattcg atgatttcat cgccatgatg
     6841 aaagcggctg cgaagtgagt ttttgcattt tccgaattga gattcgccat gatgcggccg
     6901 ggccggagcg attctgaagt tgcacgaatt ctttacacca acaagcgctt gcgccgccac
     6961 gtgccactcc ctaagatgga gcgccatgat tagcgaaccg cgggcgcaca aaagaacgac
     7021 ggcgactggc gccgagtcaa tgttgcgacg tctgaagatg ggtttccaag gaaaagtcca
     7081 caggagcatt cgtttgcact cggcttgtgg tccggtagtc tggacgtcat gacgcgatac
     7141 ctaaaattca gaaagtcgaa gcggacgcgc gaaggggtga acttctgcca gtcgcagcta
     7201 tggcggcccg gcctgcggca agtcgggctt gccatcccta aggagggtga agggcatata
     7261 acctcggctc cgaagctgca tttctgctcg acacttctgc cgagtagaaa cggcggtatc
     7321 ctgctgaaaa caaaggatcg gtgtacaggc tacccgtcac aacatcgcgc gggcgcctgg
     7381 atgctttttc gcgcagtcga caccggcttc ctcttcaaaa tattacgcgc gctgagcgtc
     7441 gaacctgctc ctttcgcaac caaagagatc catgccgaaa cgcactgacc tcaaaaagat
     7501 cctcatcatc ggctcgggac cgattgtgat cggccaagcc tgcgagttcg actattccgg
     7561 cacgcaggcg tgcaaggctc tgcgcgcgga gggctacgag gttgtcctcg tcaattcgaa
     7621 tcccgcgacg atcatgaccg accctgaaat cgccgaccgc acgtacatcg agccgctcgc
     7681 taaggagtat ctggaagcca tcatcgccgc ggaaaggcct gatgcgctgt tgcccaccgt
     7741 cggcggccaa actggtctca atctggccgt cgagcttgct gaagcgggca tcctcgaaaa
     7801 gtacggagtc aagctgatcg gcgcgcagct cgatgccatc aaaattgccg aggaccggct
     7861 gctcttcaag gacgtttgcc gaagcgttgg tctagaagtt cccgcatcgg ccgtcgtcac
     7921 caacattccc gaagcgattg atctcgccga caagctgggc tatcccgtgg tgattcgtcc
     7981 ttcgtttacg ctgggcggca caggcggctc gatcgcttac aaccacgagg agtttggcga
     8041 cgccatcgcg cgcgcccttg atgccagccc agtccggcag gctttgatcg aggaatcggt
     8101 gatcggttgg aaggaattcg agctggaggt gatgcgcgat tttcgcgaca acttcgtggt
     8161 gatctgctcg attgagaatg tcgatcccat gggaattcat accggcgatt cgatcaccgt
     8221 ggctcctgcc cagacgctta ccgacaagga atatcagcgc atgcgcgacg cggccgccgc
     8281 aatcatccgc gccgtgggcg tggagacggg cggttcgaac atccagtttg cgatcgaccc
     8341 ggaaacggga cgcatggtgg tgattgaaat gaatcctcgc gtgtcgcgaa gttccgcgct
     8401 ggcgagtaaa gccaccgggt tccccatcgc gaaaatcgcc gccaagcttg ccgtgggcat
     8461 gacgttggat gaaattccca acgacatcac gcgcaagact ccctcgtgct ttgagccgag
     8521 catcgactac gtggtagcga aaattccgaa gtggcaattc gaaaaatttc caggcagcga
     8581 gacgacgctc accacgcaga tgcggtcggt gggagaggtg atggccatag ggcgcacgtt
     8641 tcaggaatcg ctctgtaagg cgattcgttc gctggagccg agcacggcct ggcgcgctcc
     8701 ggctgaagtg actaccgcct tgcttcgcga aaagctccac gtgcccgggc cggaccgcat
     8761 ccactggctt ttcgaagcgc tagagcgcgg catgacgcgc gacgaagtct gctcgctgac
     8821 caaaattgat ccatggttca tttgccagat ggaagaaatg gttgcgctcg accggcgcgt
     8881 ggccgccagc tcgctccgcg gaatttcgcg cgaactgctg ctcgaatcaa agcgctcggg
     8941 cgcgagcgat gaggagcttg ccaagttgtg gaatgtgaaa tcggcctcgg tccgcgcgcg
     9001 ccgccacgaa ctcggggtgc tccccgtttt caagcgcgtg gatacctgcg ccgcggaatt
     9061 cgaatccttc acgccgtatc tttactccac atacgaagaa gaggacgaat ccggcgccgt
     9121 ggaccggccc aaaattgtga tccttggcag cgggcccagc cggatcggac agggaattga
     9181 attcgattat tgctgctgcc acgctgcgct ggcgctgaag gaagatggct acgagacggt
     9241 gatgattaac tgcaatcctg agactgtatc gaccgactac gacacgtctg accgccttta
     9301 tttcgagccg cttacgcttg aagacgtgct ggccgtcctc gagcgcgaaa aaccgcaggg
     9361 cgtaatcgtg caattcggcg ggcagactcc gttgaatctg gctctcgaac tgaagcgcaa
     9421 ccgcgccaaa ataatcggca ccgatccgga gtccatcgac ctcgctgaag accgccggcg
     9481 attcggcgcc ttgctcgcgg aattgaacat tccacagccg cgcggcgcca cggcgctggc
     9541 cccgcaggaa gccgtcgtca ctgccgccga aattggtttg ccggttctgg tgcggccgag
     9601 ctacgtcctc ggcggccggg ccatggtgat cgcctacgat ttgaagaccg tggaagaata
     9661 cgtgatgcac gccgcgcaga ttggcgggat ctccgatccg aagcggccga tattgatcga
     9721 tcagttcctc gaaaacgcaa cggaagtgga tgtggacgct cttgctgatg gcgacgatat
     9781 tgtgatcgcc ggaatcatgg agcacatcga ggaagcaggc attcattccg gcgattcctc
     9841 atgcgtgctg ccacctgtaa ccttaaagcc ggaggtgctc gcacatattc gcgactacac
     9901 gtcgcgcctc gccaaggcac ttcacgtagt gggcctgatg aacgtgcaat acgccatcca
     9961 gcacgatact gtttacgttc tcgaagtaaa tccgcgcgcg tcgcgcactg tgccatatgt
    10021 gagcaaagct accggggtac cgctcgcgaa agtggcggcg cgactaatga ccggccgtaa
    10081 gctgaaggat cttcgtctgc cggtgattca gactaatggc atccaggaac ttgcggtccg
    10141 cgatttctac gccgtgaagt caccggtctt tcccttcaat aaattccggg gcatcgatac
    10201 gattctcggc cccgagatgc gctcgaccgg cgaagtgatg ggaatctcat caactttcgg
    10261 cttggccttt gccaaggcgc agcttgcagc aggccagcgc ctgccgctcg agggcatggt
    10321 ttttatcagt gtaaacgatc acgacaagcg acacgtggct agcgtggtta aggatcttgc
    10381 tgtagcggga ttgaaaattc tcgccacgcg tggcacggcc acggcgctca cgcgggcggg
    10441 gatcgaagtc gaggcggttt tcaaagtaaa cgagggccgg cccaacgtgg tcgacctcat
    10501 caagacggga aaaatccaac ttgtgatcaa cacgccgctg gggcgcgagt ccttctacga
    10561 cgaacgctcg atccgccgcg cggccgtccg ttacaacgtg ccctgcatca ccactctttc
    10621 cgccgcgagt gccgccgctc gcgggatccg cgctatggcc ggccacgctg tcgaagttgc
    10681 cgcgttgcag gatttgcatc cccgcaagtc ggccgccctg ccgtgattgg gtgtggatga
    10741 aatgctcgca tcactcttgc tcctcggaaa ctgttaggcg agggcatgct ttagggaagc
    10801 gggattccgg gtgaggcgac aatgccggcc attcgcagta tttccgcgta ctcttcttcg
    10861 gtcagcggac agatggtcag ccgcgtattc ttcaggaact tgatttttcg cagcaaacga
    10921 ttctcgcgca tttccgcgag cctcactacc cgtggtagcc gttcgatggc tcgcaggtcc
    10981 attacgtatt tttttgcgtc cgcatcgtgc ggatcggggt agggatcgcg ggtcacttcc
    11041 gcgagagcgt acagcgagtg ctcgggcttt ccgtggtagc acaaggcgcg gtcgcccttg
    11101 tggacttgct tgagctgccg cgcggcatcc gggcgattgc ctgcgtgcct ccacatctcc
    11161 ttgcccttca cgaagagtgt atcccaatgg tagacgcgtg ggtcaacggc cagcagccac
    11221 cgcttgggtt ttgggggagg ttgcgtcatc cgtcaaaaga acgtcgcgcg ggaactatac
    11281 acggaagtga tggaaaaatg tgcgcaaatt ttctgtc
//
LOCUS       DATNDT010000345         2189 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_569491, whole genome shotgun sequence.
ACCESSION   DATNDT010000345 DATNDT010000000
VERSION     DATNDT010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569491"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VN788_11160"
     CDS             <1..247
                     /locus_tag="VN788_11160"
                     /inference="COORDINATES: protein motif:HMM:NF020034.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HXU21131.1"
                     /translation="MILTSTVADKVGHTTQVCVKPGHHGNGIGRQLMEHSIRALRRLH
                     YESLSLTVTSVNTHAVELYQHLGFRTVKTFAAGVWQA"
     gene            complement(262..726)
                     /gene="nrdR"
                     /locus_tag="VN788_11165"
     CDS             complement(262..726)
                     /gene="nrdR"
                     /locus_tag="VN788_11165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HXU21132.1"
                     /translation="MKCPFCSHLDDKVIDSREARTGDLIRRRRECLKCNRRFTTYERI
                     DEIPYMVIKKDGRREKFERQKILQGLLKACEKRPVPVGKLETIVDQAEGFVSESSDRE
                     RTTTEIGELLMAQLKKLDKVAYVRFASVYSDFKDVKEFMDELKGLLKDRAKR"
     gene            complement(723..1859)
                     /locus_tag="VN788_11170"
     CDS             complement(723..1859)
                     /locus_tag="VN788_11170"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXU21133.1"
                     /translation="MLLRFKRMLQKARRYLLLIVGLLVVGFFLYRFRNSIALAGFRWS
                     MVGRSLRQAQLSLLFLSLAAIYACYAIRALRWVRFCRSIGHAHFWSVYGATLEGFACS
                     FLLGRAGEPIRPVLIARKDSLSIPGMFGVYVLERVFDMAAAVLLLVFALLSFTEGGFA
                     SSEEAGLMRLARSAGVALLVGLVLAVAFLVYFRYYGGAWLARKLQHQKWHTGWREKLA
                     ALLEGFSEGLQGIRSWSDLGVVTGYTILHWILVVLIYLWIAHAFGGSLSALDLGGAVL
                     VTAITLVGSAAQLPGVGGGSQLATFLVLTLMFGVEKEPAAVASIVIWLITFAGCCLVG
                     LPLLFREGWSMGELRKMAREEERSGGAQLLADAERGVGPRENPQ"
     gene            complement(1949..>2189)
                     /locus_tag="VN788_11175"
     CDS             complement(1949..>2189)
                     /locus_tag="VN788_11175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012209379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HXU21134.1"
                     /translation="FEPVHGSAPDIAGKNIANPIGAVLAAAMMCEYLGWNDVACGMEE
                     GVRAAIRERATTVDLGGALSTQAAGDWLTNKVGSS"
BASE COUNT          528 a          705 c          547 g          409 t
ORIGIN      
        1 aatgattctt acgtccacgg tggcggacaa ggtcggccac acaacccaag tttgcgtgaa
       61 gcccggtcac cacggcaacg gaatcggcag gcagttgatg gaacattcga ttcgggcgct
      121 tcggcggttg cattacgaat cactctcact cactgtgacc tcggtgaata ctcacgccgt
      181 cgagctctac cagcacctcg gtttccgaac ggtgaaaaca ttcgccgcgg gcgtctggca
      241 ggcgtagccc gtgcgccaga cctatctctt tgctcggtcc ttcaataaac ctttgagctc
      301 atccatgaat tccttcacat ccttgaaatc cgaatacacg gacgcgaagc ggacgtacgc
      361 gactttgtcg agcttcttca actgcgccat taacaactcg ccgatttcgg tcgtggtgcg
      421 ctcgcgatcg gatgactcgc ttacgaaacc ctccgcttga tcgacaatgg tttcgagctt
      481 gcccacggga accgggcgtt tttcgcaagc cttcagcagc ccttgcagaa ttttctgccg
      541 ttcgaatttt tcgcgtcgcc catctttctt aatgaccatg tacggaattt catcaatgcg
      601 ctcatacgtc gtgaaacgcc gattgcactt caggcactcc cgtcgccgac gaatcaaatc
      661 gccggtgcgc gcctctcttg agtcgattac cttgtcgtct agatgagaac agaaggggca
      721 cttcactgcg gattctccct tggccccacg ccacgctccg cgtcggccag tagttgagct
      781 ccgcccgacc tttcctcctc gcgcgccatt ttccgaagct cgcccatcga ccagccttcc
      841 cgaaacagca atggcagtcc cactaagcag catcccgcaa acgtgattag ccaaatcaca
      901 atcgaagcaa ccgccgccgg ctctttttct actccaaaca tcagagtcag gacaaggaaa
      961 gtagcgagct gcgaccctcc tcctactccg ggaagttggg ccgcagaacc cactaatgta
     1021 attgccgtga ccagaaccgc ccctccaaga tccagcgctg aaagactccc cccaaacgcg
     1081 tgcgcgatcc ataggtagat caaaacaacc aaaatccagt gaagaatcgt atagcccgtg
     1141 acgacaccca gatcgctcca cgagcgaata ccttgcaagc cttcgctaaa gccctcaagc
     1201 agcgcggcca gcttctcgcg ccagcccgta tgccacttct ggtgctgcaa tttgcgtgca
     1261 agccacgcgc cgccgtaata gcgaaagtaa accaggaacg ccaccgccag cacgagcccc
     1321 actagcagcg ccactccggc ggaacgggcc agccgcatca atccagcctc ttcggagctg
     1381 gcgaacccgc cttctgtgaa cgatagaagg gcgaagacaa gaagcaggac tgcggcggcc
     1441 atgtcaaata ctcgctccaa gacatacacg ccaaacattc ctggaattga aagcgagtct
     1501 tttctcgcaa tcaagacggg acgaataggc tcaccggccc ggccgagaag aaaactgcac
     1561 gcgaatccct ccaaagtggc gccataaaca ctccaaaaat gggcgtggcc gatcgagcgg
     1621 caaaaccgga cccaacgcag agctcgaatc gcgtagcaag cgtaaatcgc tgcaagggaa
     1681 agaaacaata atgagagctg cgcctggcga agcgatcgcc caaccatgct ccaacgaaat
     1741 ccggctagcg caattgaatt gcggaatcta taaaggaaaa agccgacgac gaggagcccc
     1801 acgatcaaga gcagatagcg tcgcgccttt tgcagcatcc gcttgaaccg tagcagcaag
     1861 gtttcagcgg tgtcaaggag ggcagcctgc ccagattcac agtgatcggc cgaactggcg
     1921 ctggaaaacg cgtctaggcc aaaactaatt aggaggagcc tactttgttc gtaagccaat
     1981 cccccgcagc ctgcgtgctg agcgcgcccc ccagatccac cgtcgtggca cgctcccgaa
     2041 ttgccgcgcg aaccccctct tccatcccgc acgcgacatc gttccagccg agatactcgc
     2101 acatcatcgc ggcggcgagg accgcgccta tcgggttcgc gatgttcttg ccggcgatgt
     2161 ccggggccga gccgtgcacc ggctcaaaa
//
LOCUS       DATNDT010000346         3345 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3848777, whole genome shotgun sequence.
ACCESSION   DATNDT010000346 DATNDT010000000
VERSION     DATNDT010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3345
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3848777"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..927
                     /locus_tag="VN788_11180"
     CDS             <1..927
                     /locus_tag="VN788_11180"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyalkane--coenzyme M transferase"
                     /protein_id="HXU21135.1"
                     /translation="GADPFARGADRERFRDFYAELEKPNERTWSRVTTHDVVCVAPIQ
                     YTGLGELQRDIENFKAALRDVKVEEAFLPVAAPSSAIPDRKNEYYHNDEELLVALAEA
                     LRTEYKTIADSGILLQIDDARAAVTYDRMVPPGTFEDYYRWLSRHVDVLNHALEGIPE
                     DRVRYHVCWGSWPGPHTTDVPLKKIVDLILKVRAGAYLIEAANPRHEHEWRIWKDVKL
                     PDGKILAPGVVSHGTNVVEHPELVAERIVRFAEVVGRENVIASTDCGFAQEQFNRRVH
                     FSIMWAKLEAMAEGARIASKELWARSGQSTVE"
     gene            1034..2026
                     /locus_tag="VN788_11185"
     CDS             1034..2026
                     /locus_tag="VN788_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21136.1"
                     /translation="MRLKSFASRAIALALLFGGLSVTSTTRAQDYNAVPPKCDRACLV
                     EFMHKYLTALVTRDPSRVPWADKVEFTENNARLMVGDGSWAVATGKLDNDLIVADPLT
                     GQVGYFGTVKLNDVPTWFAARLKVADAKVTELETVWRPRPPVVNAAAPGGGDPKNLKH
                     DPAMYDSLEPSERVPRRKMIDLANGYFATLQLNDGTLHTEFDPTCSRIDNGTKSASNP
                     DSPNLAFRLNCGDQFKTGMYAANDAVRERDFFLVDEEKGIVMARAFLDHNAVVQTIKL
                     TDGTQIRAGFRTPNSFVMLEMFKFKNGKLYRAEVVHADTNYHASSPWTPPSRRD"
     gene            complement(2066..2410)
                     /locus_tag="VN788_11190"
     CDS             complement(2066..2410)
                     /locus_tag="VN788_11190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21137.1"
                     /translation="MWKKYEEGSQLGSAEAVMAYEQAMREFAMSAAEFLEHIQLLTKT
                     RDAYQRAVTISAQLRGTLDKGDETLRNLMSQVDHAVSGQRGQDVPNGNKPEATKVETD
                     NASVEKADAARA"
     gene            complement(2712..>3345)
                     /locus_tag="VN788_11195"
     CDS             complement(2712..>3345)
                     /locus_tag="VN788_11195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXU21138.1"
                     /translation="WGLDENAHSPVLAQFYFPISQIPDQLMPLVARTVSADVRTDGSP
                     LAVIGSLRHALEQFNSQMVMYGAQPLEEVISDSLASRRFSMILLGVFAAIALVLSAIG
                     IYGVISYVVGQRTHEIGIRLALGAHPGDVMRLILGQATRLALIGVAIGIAAALALTRL
                     MTKIIFGVNAHDPLTFAGVAFVLVIVALAACYIPARRAMRTDPMVALRYE"
BASE COUNT          725 a          973 c          982 g          665 t
ORIGIN      
        1 ggcgcggacc ctttcgcgcg gggtgcggat cgagagcgat ttcgcgactt ttacgctgaa
       61 ttggaaaaac ccaacgaacg cacctggtcg agggtgacca cgcatgacgt agtctgcgtg
      121 gcgcccatcc aatacacggg cttgggcgaa ctgcagcgcg atatcgagaa cttcaaggca
      181 gcgctgcgag acgtgaaagt cgaagaggcg ttcctgccgg tggcggcacc ctcaagcgcc
      241 atcccggacc ggaagaatga atactaccac aatgacgagg agctgctcgt cgcgctggca
      301 gaggccctgc gaaccgaata taagacgatt gccgattccg ggattctgtt gcaaatcgac
      361 gacgcacgag ccgcggtgac ttacgatcgg atggtgcccc cgggaacctt cgaagactac
      421 taccggtggc tgagccgtca cgttgatgtg ctgaaccacg ccttagaagg gattccggag
      481 gaccgggttc gttaccacgt gtgctggggc agttggccag ggccgcacac cacggatgtg
      541 ccactcaaga aaatcgtcga cctgattttg aaggtgcgcg cgggcgcgta tctgatcgaa
      601 gcggcgaatc cgcgccacga gcacgagtgg cggatctgga aggacgtcaa acttcccgat
      661 gggaagattc tggcacctgg cgtggtgagc catggcacga acgtggtaga gcatccggaa
      721 ctcgtcgccg aacgcatcgt ccggtttgcg gaagtcgtcg ggcgcgagaa tgtaatcgcc
      781 agcaccgact gtggatttgc gcaggagcag ttcaaccggc gggtgcactt cagcatcatg
      841 tgggcaaaac tggaagccat ggccgagggc gcgcggatcg ccagcaagga actttgggct
      901 cggtcggggc aatcgacagt ggaataaggc aagcgtcact agattgccca tttagaacag
      961 ccggatctct ggcttaaatc gctcgcattc gttaattggc tgcgacacca aaaggccacc
     1021 caacggagga ccattgagat taaaaagttt tgcgtctcgc gcaatcgcgc tggcactttt
     1081 gtttggcgga ttgtccgtca catcgactac tcgggcgcag gattataacg ccgtgccgcc
     1141 aaagtgcgac cgcgcctgcc tggtcgaatt catgcacaaa tatctgaccg ctctggttac
     1201 acgagacccg agccgggtac cttgggctga caaagtggag ttcactgaga ataacgcgcg
     1261 gctaatggtc ggcgatgggt cctgggcagt cgcaaccggc aagctggata acgatctgat
     1321 tgtggccgat cctctcacgg ggcaggtagg ctactttggg accgtaaagt tgaacgacgt
     1381 gcccacttgg tttgccgcgc gcctcaaggt cgcggacgct aaggtcacag aactggagac
     1441 tgtttggagg ccgcggcccc cggttgtgaa tgccgccgcc ccgggaggcg gcgaccccaa
     1501 gaatttgaag cacgaccctg cgatgtatga ctctctggag ccgtcagagc gcgtgccgcg
     1561 tcgcaaaatg atcgacttgg caaacggcta cttcgctacg ctgcagctca acgacggaac
     1621 tctccatacc gaattcgatc ccacttgctc gcgaatcgac aatggcacca agtcggctag
     1681 caaccccgac tctccgaacc ttgcattccg gttgaactgc ggcgatcagt tcaagaccgg
     1741 catgtacgcc gcgaacgacg ccgtccgcga gcgggatttt ttcctggtcg acgaagagaa
     1801 aggcatcgtc atggctaggg cctttctcga ccacaatgcc gtggtccaga cgatcaagtt
     1861 gacggatggc acgcagattc gtgccggctt cagaaccccc aatagcttcg tcatgctgga
     1921 gatgttcaag ttcaaaaacg gcaagcttta tcgcgcagag gtggtgcatg ccgacaccaa
     1981 ctaccatgcc agttcacctt ggacgccgcc ctcgcggcgc gattagccgg gtatcgatcg
     2041 caccggtgaa tgggatcggt taacgtcacg cccgcgcagc atcggctttt tccacactcg
     2101 cattgtctgt ctctactttc gtcgcttcgg gcttgttccc attgggtacg tcttggccac
     2161 gctggccgct gacggcatga tcaacctggg acataaggtt ccgcagggtc tcatcgccct
     2221 tatccagggt gccccgcaac tgagcactaa ttgtcacagc gcgctgatag gcatcccggg
     2281 tcttcgtcaa gagttgaatg tgttccagaa actccgcagc gctcatagcg aactctctca
     2341 tcgcttgttc ataggccatc actgcttctg cggagcctag ctggctcccc tcctcatatt
     2401 tcttccacat tgcttcgctc ctcggtttcc tcagtctggc ggcaccaccg gcaggagttc
     2461 tgaaacggaa tccccgatct ggaccgcagt ttcgcgtatc ctacgctcct ccctggcctc
     2521 cacgcaacga gtttggcgta ctctacggcc tggtactttc gggcgttcct gcagcctggt
     2581 actttccccg ttaaataaaa gaaagggcag acaatatcgc tggaacggcc tacagatccg
     2641 atcgaacctt gcgtgctgcg ctcttcgtgc gtcgcgaagg gcgtgggggt tcgcggcaag
     2701 gcaaactcgg tttattcgta gcgcagtgca accatgggat cggtgcgcat ggcgcggcgc
     2761 gccgggatat aacacgccgc gagcgcaaca atcaccaata cgaacgcgac gccggcgaaa
     2821 gtgagcgggt catgcgcgtt gacgccaaaa attattttag tcatcagacg cgtcaaggcc
     2881 agagccgccg caatgccaat cgccacgcca atgagcgcga gccttgtggc ttgcccgagg
     2941 atgaggcgca tcacatcgcc tggatgcgcg ccgagtgcga gacggatgcc gatttcgtgc
     3001 gtgcgctggc cgactacata ggaaatcaca ccataaatgc cgattgctga gagcaccagc
     3061 gcaatcgcgg cgaacactcc gagcaaaatc atcgaaaatc gacgcgaggc cagcgaatcg
     3121 gaaatcactt cttcgagcgg ttgcgcgccg tacatcacca tctggctgtt gaactgttcg
     3181 agtgcatggc gcagacttcc aattaccgcg agcggggaac cgtcggtccg aacgtctgcg
     3241 cttaccgtcc gagccaccag cggcatcaat tggtccggga tctgcgagat cgggaaataa
     3301 aactgcgcga gtacagggga atgggcattt tcatcgagcc cccac
//
LOCUS       DATNDT010000347         3919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3552757, whole genome shotgun sequence.
ACCESSION   DATNDT010000347 DATNDT010000000
VERSION     DATNDT010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3919
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3552757"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(226..387)
                     /locus_tag="VN788_11200"
     CDS             complement(226..387)
                     /locus_tag="VN788_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21139.1"
                     /translation="MARRKKPRLNVAREARRRARRGVGLPPPERIIPNKQSKPLKHKK
                     PLATLLNEE"
     gene            complement(649..921)
                     /locus_tag="VN788_11205"
     CDS             complement(649..921)
                     /locus_tag="VN788_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21140.1"
                     /translation="MLARRSDNTALSGDTYFVVIGSHLYTSRELKRAFYSSRPVFVAG
                     RAGLHIYPATPNVLTIECEGCGITKDLVEKQRSSDDGIIVRYVGFP"
     gene            complement(1419..>3919)
                     /locus_tag="VN788_11210"
     CDS             complement(1419..>3919)
                     /locus_tag="VN788_11210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21141.1"
                     /translation="ETRTAPPAAHPARMKLEPYSAEIRLANLTVGVDNIHYDVFLSPR
                     FVEFVRTYLFDLVRQVVNVGLLYGNDKGRKPPKPPEHSAFRKLLTEILQESLTRAKFQ
                     QSIETDVLHHLALLKFITQETGNQFSSILVECKDYIRGRGQLFEHSEQAHVLRSKIAE
                     IQADRKNIIRQVGETLCRVWREVEDTTIARTRRALFGDDSDQMYPLLQNRFLFVEGGN
                     DEYLFLEHYVLLGNFINDPDRFEIFDALLLDIVRECVLAGDNAGDSAEELTKARKLYE
                     RLVEQARLLHAELALIEEEHEEAAGRAGESGDLFPSIFKRKAAHDPGAHSEMDNLRRK
                     TASLEKNLEELAPQVAAAKQRMDFLNAEFQGRLGDYLNQPANARRLFDAGAGADENSA
                     AAAEIRAHLLEEWIHRLEERELLFHVLAGYELRKIATEYCPPVHLQQLKKALVLRDEA
                     KRVAQILEKFPARKVSMKKFEQSSRAIRRRSAQETLAIGLQFAEDLMRLRRDRRNYQQ
                     VTAWMERINLVHSERARELSRVNKSLYEFLHPEEGRPADDPVINHVVIKADVRGSTGI
                     TKDLLERGMNPASHFSMALHEPVKRMLERFGAAKVFIEGDAIILAIYENESTRATQRA
                     VARACVLAREILAVTQAYNARAKSGNLPPLELGVGVAFQDSAPSLWMDADSRIMISRA
                     LNLSDRLSSCSKMAKRLFQANPAPFNVYLLQTLMDDTTAEEGEELLVRYNLNGIELNE
                     EGFQKLQREISLTPVGGNFPLPWGKERVQLYFGEVPLGESLEPVVIRKGLVRQLLPGG
                     KIGDQGARAYYEVCTDAKLLDLARRKVSAATPKN"
BASE COUNT          825 a         1072 c         1204 g          818 t
ORIGIN      
        1 gttatgtggg tttctactcg gcagatgtgt cgaatagaaa ccgcaggcgt ggttttggtc
       61 cgggctacgg ggcgggggcc ccggcgagac cggcggcttg cacgtttcgc gcatcgtccc
      121 catggtacgg caggtcgtag gtcgcgcaag gatggatccc tgtggacttt cgtcgagaca
      181 gcacagtcgt tcgggcgatg gcgacagaat ctttggtgag cgcagctatt cttcgttgag
      241 cagggtagcg agcggcttct tgtgcttgag aggcttggat tgcttattcg gaatgatgcg
      301 ctccggcggc ggcaggccga cgcctcgacg cgctcggcgg cgcgcttccc gagcgacgtt
      361 cagacgcggc ttctttcggc gtgccacctt cgagtttccg ctagagatta gaagccacct
      421 gagtcgagta gtcaaacatg gctcagcgcg tcggggttcg gtgtggctga tccgattgaa
      481 gcgaaggtcg cgcgggtgaa gcagcgcgag cggaagcgac ttcgtcggtc gattcatcag
      541 gaaagaccgg gaagaagccc actctcgcag aacggcgagg atgaggcacc ctcaaattca
      601 gaacaagcgc agcacccggc cacccgcccc tcagcgaaat tggacggtct aaggaaaccc
      661 aacgtagcga acgataatgc cgtcatctga agatcgctgc ttttcaacca aatctttcgt
      721 gatgccgcag ccttcgcact cgattgtcag tacattaggg gtggcagggt aaatgtggag
      781 gcctgctcga ccggcaacga aaacaggtcg cgaactatag aaagctcgtt tcaactccct
      841 gcttgtataa agatggctgc cgatcaccac aaaataggta tcaccgctga gagcggtgtt
      901 gtccgatcgc ctggctaaca ttgcaacatg gttttgcgaa accgtttgag attgcgcaat
      961 ctcgactttt ccggaatcca ataccccatg aacgccaacg caagccagat ggcgatgaga
     1021 cagacaactg tcaccagaga tatccaggtc aaaaagcgtc tacgcgattt cactcgtgac
     1081 accccgctgc cctcatagcc tgacctagct caattggggc gttgtcgctt ggagtgtcca
     1141 tgaaagtacg gggcgaacca aatgaagctt ttccaaatgg cgccgccccg aactgagcgg
     1201 caccggcaag cggcttggca aaaaatagcg ccatatggac acgtgacacc aaagccgatg
     1261 ttgccggcat caatgcccgt ttggttgtga tatcccgggc ccggctgatt cttaagatcg
     1321 ttaggccctc cgttatgtac cgccccacag taacctgaag cgttgctttc cccggataaa
     1381 tgcggggggc ttccaccaag cacagccagc ggacggcgct agttttttgg tgtggcggcg
     1441 gaaacttttc tgcgggcgag gtcgaggagt ttggcgtcgg tgcaaacttc gtagtacgca
     1501 cgcgcgcctt gatcgccaat tttgccgccg ggcaacaact ggcggacaag acctttgcgg
     1561 atgacaacgg gttcgagaga ttcacccaga ggtacttcgc caaaatagag ctgcacgcgc
     1621 tccttgcccc aagggagcgg gaaattgccg cctacgggag tgagcgagat ttcgcgctga
     1681 agcttctgaa aaccttcttc gttgagctcg atgccattca gattgtagcg gacgagaagc
     1741 tcctcaccct cttctgccgt agtatcgtcc atcagcgttt gcagcaggta gacgttgaaa
     1801 ggggcgggat tcgcctgaaa gagacgcttg gccatcttcg agcagctcga gagacggtcg
     1861 gacagattca gcgcgcgcga aatcatgatg cgagagtcgg cgtccatcca aagcgagggc
     1921 gcggaatcct gaaacgcaac gccgaccccc agctcgagcg gcggaagatt gccggacttc
     1981 gcgcgcgcat tgtaagcctg ggtgacggcc aaaatttcgc gggccagcac gcaggcgcgg
     2041 gccacagcgc gttgggtggc acgagtggac tcgttctcgt aaatcgccag gatgatggcg
     2101 tcgccttcga taaaaacttt ggcagcgccg aaacgttcca acatgcgctt cacgggctcg
     2161 tgcagggcca tgctgaaatg ggaggcggga ttcatgccac gctcgagcag gtccttggtg
     2221 atgccggtgg aaccgcgaac gtcggctttg atgaccacat ggttgatgac tggatcgtct
     2281 gcaggtcgtc cctcttccgg atgcaggaac tcgtagaggc tcttgttgac gcgggaaagt
     2341 tcgcgggcac gctcggagtg gaccagattg atgcgctcca tccaagcggt gacctgctga
     2401 tagttgcggc gatcgcggcg cagacgcatc aagtcttcgg cgaactgaag gccgatcgcc
     2461 agcgtctcct gcgcgctacg tcggcgaatg gcccgggagg attgctcgaa tttcttcatg
     2521 gataccttgc gcgccggaaa tttttccaga atctgcgcga cgcgcttggc ttcgtcccga
     2581 aggacaagcg ccttcttcaa ttgctgaagg tgaacgggag ggcaatattc ggtcgcgatt
     2641 ttgcgcagtt cgtagcccgc gaggacatgg aagagcaact cgcgttcctc gagccggtga
     2701 atccactcct caagaagatg ggcgcggatt tccgcggcgg cagcagaatt ttcatccgca
     2761 cctgcgccgg cgtcgaacag gcgccgggcg tttgcgggct ggttcagata atcgcccagc
     2821 cggccctgaa attccgcgtt caaaaaatcc atgcgctgct ttgcggctgc gacctgcggc
     2881 gccaattctt ccaaattctt ttcgagcgaa gcggtcttgc ggcgaaggtt atccatctcg
     2941 ctgtgcgcgc cgggatcgtg agccgccttg cgcttgaaga tggaagggaa caaatcgccg
     3001 ctttcacccg cgcggccagc ggcttcctcg tgctcttcct caatgagcgc gagttcagca
     3061 tgcagaaggc gcgcctgttc gaccaggcgc tcgtacagct tccgggcttt ggtaagctct
     3121 tcggccgagt ctccggcatt gtcaccggcg agaacgcatt cgcggacgat atcgagaagc
     3181 agcgcgtcaa agatttcgaa gcgatccgga tcattgatga aattaccgag cagcacgtaa
     3241 tgttcgagaa agagatactc gtcgttgccg ccttcgacga aaaggaagcg gttctgcagc
     3301 agcggataca tttggtcgga atcgtcgccg aagagagcgc ggcgcgtcct ggcgatggtg
     3361 gtgtcctcca cctcgcgcca gacgcggcat aaagtctcgc cgacctgacg gatgatgttt
     3421 ttgcggtcgg cctgaatttc ggcaatcttg gagcgcagca cgtgagcctg ttcgctgtgc
     3481 tcgaagagct ggccgcggcc gcgaatgtag tccttgcact cgaccagaat gctcgagaat
     3541 tggtttcccg tttcctgggt gatgaatttc aagagcgcga ggtgatggag aacgtccgtc
     3601 tcgatggact gctgaaattt ggcgcgcgtc aaggattcct gcagaatttc cgtgagtaac
     3661 ttccggaagg cggaatgctc gggcggcttg ggaggcttgc gtcccttatc gttgccgtaa
     3721 agcaagccca cgtttacgac ctgcctaacg agatcgaaga ggtacgtgcg cacgaattcc
     3781 acgaaacgcg ggcttagaaa aacgtcataa tggatgttat cgacaccgac ggtcaggttg
     3841 gcgagccgaa tttcggcgga gtacggctca agcttcatcc gggctggatg ggcggcaggt
     3901 ggcgcggtac gcgtttcca
//
LOCUS       DATNDT010000348         2841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2592014, whole genome shotgun sequence.
ACCESSION   DATNDT010000348 DATNDT010000000
VERSION     DATNDT010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2592014"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            375..929
                     /locus_tag="VN788_11215"
     CDS             375..929
                     /locus_tag="VN788_11215"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXU21142.1"
                     /translation="MPSTSAPATSAAPIVQGGMAARDTGLYAGFWLRVVAAIIDGLLI
                     GIPFIVIAVVIFASALPMIRNFGSEPNPNPFLLISLFFPRLLLLAALGLVGTWLYWSL
                     LESSSWQATLGKKALGLYVTDLTGAPISFGKASGRFFAGRGIAYIPSIGGLYFLVDCI
                     MAGVTEKKQALHDMIAGCLVQRNG"
     gene            complement(1041..1976)
                     /locus_tag="VN788_11220"
     CDS             complement(1041..1976)
                     /locus_tag="VN788_11220"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HXU21143.1"
                     /translation="MANHVLSAEPIHSRWNRDLPPRLTIASGDTVHFECLDASGAQVR
                     PGMTLDEYIEINTERIHALTGPIFVEGAAPGDVLQIDILEVAHKGWGWSSVISGLGFL
                     KHKFADPYLFHWTLEPEVSRSLAPAIVPLRPFCGVMGVAPAERGEFRTIPPGPFGGNM
                     DVRELCAGSTLYLPVLNRGALFSAGDAHAAQGDGEVCVNGIECPADVTLRFRVHNQRP
                     LRGPFIESNSRNAPPESEGWIVVESATNAIDAARAATSRMIDLLVEHWNFSEIHAYIL
                     CSVAMNLRLSQVVNEPMFTVSAALPKRVLPAKQIF"
     gene            complement(1986..2150)
                     /locus_tag="VN788_11225"
     CDS             complement(1986..2150)
                     /locus_tag="VN788_11225"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXU21144.1"
                     /translation="MPGFTDSPKVINGASDLLVEIFGDSGRHVRAAIGVSALPRNALV
                     ELQMTVELEA"
     gene            complement(2120..2449)
                     /locus_tag="VN788_11230"
     CDS             complement(2120..2449)
                     /locus_tag="VN788_11230"
                     /inference="COORDINATES: protein motif:HMM:NF025939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXU21145.1"
                     /translation="MTKEAEARLANLGIILPAVPKAGGNYIPAKTVGHIVYLAGAIST
                     NSEGVITGTVGLNRTIDEGYAAARSCAPTQLAVLKRHLGSFDPVKSIISANGYVNACR
                     ALPIRRK"
BASE COUNT          602 a          790 c          849 g          600 t
ORIGIN      
        1 gacaaataga tccaaggtct tccctacctg cggctattgt ggtccagcga atcgaaaagt
       61 gtacgctgca acgcctcatc tttgccagac aaccttcgcg cgcactcaaa gcaccgagta
      121 accctggcat ttttcgcttc cggcagcgta attgtcgaac gccggtcctg gtgttgaccc
      181 ttctgccttg agtcggtacg atgcggagca cgtcaaggct cctagctgga gctgaatgca
      241 tttctccggg gggcacaatg ttctgctcgt cgtgcggcgc ggcggtaaca gagggttcga
      301 cgttctgcgg gaattgcggc agaccagcta gttacagccc ggctcaggca gcgagcggag
      361 cggcgaccgg aagcgtgccc tccactagtg cgccggctac gagcgcggcg ccaatcgttc
      421 agggcggcat ggcggcgcga gatacggggc tgtacgcagg cttctggctg cgcgtggtcg
      481 cagcaatcat cgacggcctg ctgattggca ttccgttcat tgttatcgcg gtggtgatct
      541 ttgcgagcgc ccttccaatg atccggaatt ttggcagcga gccaaatccg aatccattcc
      601 tgctcataag cctatttttc ccacgattat tgctgcttgc cgctttaggc ctggttggca
      661 cctggttgta ctggagcctt ctggaaagct cttcatggca agcgacactt ggcaagaaag
      721 cgctggggct gtatgtgacc gatttgacgg gagcgccgat tagttttgga aaagcgagcg
      781 gacgcttttt cgccggtcgt ggaatcgcgt atatcccgtc gatcggcgga ctttatttcc
      841 tggttgattg catcatggcg ggcgtgacgg aaaaaaaaca ggcgctacac gacatgatcg
      901 ccgggtgtct agtgcaacgg aacgggtaat ctcgctgggc attccccgaa tccgcaaata
      961 gcatttcaat gggcctttcc gataggacac caggtgaatt atctaaaaac gcgacgactg
     1021 atccgatctg cctatagcga tcagaagatt tgtttggcgg gaaggacgcg tttgggaagc
     1081 gccgcgctga cagtgaacat cggctcgttc accacctggc tgaggcgcag attcatggcc
     1141 acgctgcaca gtatgtacgc gtgaatctca ctgaagttcc aatgctccac gaggagatcg
     1201 atcatgcgcg aagtggcggc acgcgcggca tcaatcgcat tggtcgccga ttcgactacg
     1261 atccaccctt cggattccgg gggtgcgttt cgcgaattcg attcgataaa aggcccgcgg
     1321 agcggccgtt ggttatgcac gcgaaagcgc agcgtaacgt ccgccgggca ctcgataccg
     1381 ttcacgcaga cttcgccatc gccctgggcc gcatgggcgt cacctgccga aaaaagagca
     1441 ccccgattca acaccggcaa gtagagggtg gacccggcac aaagttcgcg gacatccata
     1501 ttgccgccga aaggacctgg tgggatggtg cggaattcgc cgcgttcggc cggagcgacg
     1561 cccatcacgc cgcaaaaagg gcgcagcgga actatcgcgg gcgcgagcga gcggctcact
     1621 tccggctcca gcgtccagtg aaaaagatac ggatcagcga acttgtgttt tagaaaaccg
     1681 agtcctgaga tcacgctcga ccagccccag cctttgtgcg cgacttcgag gatatcgatt
     1741 tgaagaacgt cacccggcgc ggcgccctct acaaaaatcg gtccagtgag agcgtggatg
     1801 cgttcggtgt tgatctcgat gtattcgtcg agcgtcatac ccgggcgcac ttgcgctccg
     1861 cttgcgtcaa gacattcgaa atgcacggtg tcgcccgaag cgatggtgag gcgtggtggc
     1921 agatcgcgat tccagcgcga gtggatgggt tcggcggaga ggacgtggtt ggccatttat
     1981 aaattctacg cttccagctc gacggtcatc tgcaactcga cgagcgcatt tcgcggcaat
     2041 gcagagaccc cgattgcagc gcgcacatga cggccagagt cgccgaagat ttcgacgagg
     2101 aggtccgagg ctccattgat tactttcggc gaatcggtaa agcccggcac gcattcacgt
     2161 agccgtttgc actaatgatg cttttcacag ggtcgaacga gccgaggtgc cgcttcagaa
     2221 cagccaattg agtgggcgcg cacgatcgcg ccgcagcgta accttcatca atcgtacgat
     2281 tcagacccac ggtgccggtg atgacgccct cggagttcgt cgaaatggcg cccgcgaggt
     2341 acacgatgtg gcccacagtc ttcgcaggga tgtaattccc tcccgctttg ggaacggcgg
     2401 gcaaaataat gcctaaattc gcgagccgcg cttccgcttc cttcgtcatg gaatgtgact
     2461 gcctccgcaa agcgacttga aactctaaca tagacagctc gtcgccagat tcccgattgc
     2521 gacgacgagc ccgtgcgatg gcatgtgcca tgcaacgacc gtggatcggg caccgaatgt
     2581 tgtttcatca ggagcaggat gcaatgcgca cgaattcgct gcgtatcacg tgctcatccc
     2641 gcgtaaaggg taatgaccgg tcagcaccga gtcgcctttg gacgtcgctc tgaaaacggc
     2701 aatggtgagc gcccggcgaa aaaccatcgg caagcgctag taggagagtt tattcggctg
     2761 ctgtctccag atgcggaacg cttcgagtgc ttcgtcgcgc atcagttgaa ttatgggaat
     2821 tttgcgctgg gaatgtggcc g
//
LOCUS       DATNDT010000349         8376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2740536, whole genome shotgun sequence.
ACCESSION   DATNDT010000349 DATNDT010000000
VERSION     DATNDT010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8376
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2740536"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1094)
                     /locus_tag="VN788_11235"
     CDS             complement(<1..1094)
                     /locus_tag="VN788_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU21146.1"
                     /translation="MSARILVIEDEEKLRRVIELQLKGAGYEVAQAGTAEDGARLADT
                     ADVILTDLRLPGMNGLQFLAKLREQNLYTPVIVMTAYGTVETAVEAMKAGAADFLEKP
                     FSLEHLLARVAKALEIRSLRDENRKLREELGARYEFDNLVGHSPSMQEIFATVDRVAP
                     TRATVLLAGESGVGKDMIARAIHHHSPRASKPFVKINCTAIPENLMESELFGYEKGAF
                     TGANTSKPGKFEQADTGTVFLDEIGDVPASVQVKLLRVLQEREFERLGSNRTRQIDVR
                     VIAATNVDLRAALEQGNFREDLYYRLNVVPINVPPLREHKEDIPFLAEYFVKKLAKDL
                     DSGVTSISDDAIRRLMQYHWPGNVRELQNV"
     gene            complement(1091..2341)
                     /locus_tag="VN788_11240"
     CDS             complement(1091..2341)
                     /locus_tag="VN788_11240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21147.1"
                     /translation="MEPLAWPRPLIRLIRVRDLLWIVLFSALALFGPDRTTMMSALLF
                     SLAIYQVAESKIPWFLTQTGSAISFLIKLVLCYLLIGWTDGVASSYYWILLFPVISAA
                     TSLNLGETILAAAAASLAYLSFLLRLKPDQYIPPDQIKELYLRVLLLLLVSVLTHHLV
                     ESSLTQARYQAAAEKLAEANRNLEVAEASVRRSERLAAIGQLSAGLAHELRNPLHTMK
                     TSAELLLQSVAGENEIAREMAGFISSEVDRTNSLVTRFLDFARPLAVHAELADVHQAI
                     DRAIQQVERHDPPLDAVIYRNYSPDVRPFPFDSELMERVFYNLILNAAQASPAQGAVT
                     VKTRRLNGSIEIAVIDRGSGIDKANLESIFNPFFTTRSSGVGLGLPIVSKIVDQHGGA
                     ITVESEKGEGSVFRVLLPVRPEEA"
     gene            2394..4091
                     /locus_tag="VN788_11245"
     CDS             2394..4091
                     /locus_tag="VN788_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HXU21148.1"
                     /translation="MNSSNVSAFPLDSDVPPSGDVSFGSILSQFEEGQHDKPGEGVRG
                     TVVSLGPDSVFVDIGRKMDGVLPIEKVRDENGAISVRVGDELVVSITGRDQEGNYLLS
                     TLEVARPKDWSAFEKAFADKTPIAGTVTELVKGGLRVDCGVKAFLPASRSGARDQAEI
                     EKLVGQEIQCRIIKLDIADEDVVVDRRVILEEEERKAKDRAFDTLQEGAVLRGVVRSL
                     TDFGAFVDLGGVDGLLHVADMSWTRVTKPSDVVSPGDSIEVKVLKVSPENRRVSLGLK
                     QLAADPWTIALEKLKVGDRVQGKVSRLTDFGAFVELMPGVEGLIHISEMSWTKKIRKP
                     ADIVNPDDLVDVVILGVKPAEHRISLGLKQALGDPWEEAERKFAPGTVSEGPVTSLTN
                     FGAFVDLGNGIEGMIHISDISGKKRLNHPREALTVGETVKAAVLEMDKAKRRIRLSIK
                     RLEPTSFDEYAAEHKIGDVVTGRVIEASATKARVELGEGVSADCSLTAPAPNAPKSSA
                     VKPDLGAVTALLTAKWKSGGGSQQQLSGSGVRQGQVQSFRILSLDAPHKRIELELAE"
     gene            4213..4782
                     /locus_tag="VN788_11250"
     CDS             4213..4782
                     /locus_tag="VN788_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXU21149.1"
                     /translation="MDEAALIRAAQQGDESAFEQLVRIHDQNVLRLALNLLRSQEDAR
                     DVYQEAFLRVYRNLNAFRFDCSFHTWLYRIVTNLCVDALRKRRVRREESSVLATPDGM
                     LDRMDTVEEARADGDPQRHLLSRQLNGRIQLTLESLTPRERIVFEMRHYQGLRLRAIG
                     DALGTTEEAAKNCLFRATQKMRAALGDFL"
     gene            4779..5705
                     /locus_tag="VN788_11255"
     CDS             4779..5705
                     /locus_tag="VN788_11255"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HXU21150.1"
                     /translation="MRCDEVRKIAPLFLYGELSFDGEEQFEQHLEGCPDCRRFLEREK
                     ALQSCLDEVESDLPAGLLGTCRRDLRASLESSSGHPGHWERWREAFAIRWIPLPSFAQ
                     PAGAIALVLFGFFAARFYFGSISPGSSDAQHAALTAPLSTRIRYLEPDGSGHVQIVVD
                     ETRQRVLTGDADDESIRRLLLTAAQDPRNPGLRVESVDLLKNHMESAEVRRALLTALQ
                     HDPNAGVRLKALDGLKQFAADPETRQVLMHVLLADKNPGVRTQVIDLLVRQKNEKMVG
                     VFQELMHKEQNGYVRLRCERALREMNASLGTF"
     gene            5722..6885
                     /locus_tag="VN788_11260"
     CDS             5722..6885
                     /locus_tag="VN788_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21151.1"
                     /translation="MMPALAVGLLTGVLSFGQSSTAVVRDGSWWTQTSKGSIAASGVE
                     RVRIHAEGGVALLGNSGTSVSYSLKERVRASNSARAESRLRAFRIESRRVGAALHLTF
                     TTPTAGYTSAELTVAVPRDLPIVEIESLGGDASATNFDGILRLVTGGGQIDADRLQNC
                     FFKTAGGDIRIGLVAGPLQCYTGGGTIHVSRASGDAHLETAGGDIDVGEIDGGLTALT
                     GGGNIHITRSLQAVVAKTRGGLIEVQHAGGLVTAQTAAGSIQVTSSQGVHCASTNGTI
                     RLRNVAGVLRASTESGSILAELRPGESLGDSTLSTNAGDITVLIPPHMALTVFARNES
                     RGATGRIISDFPEIHSAAYHGLLPQTAQGSLNGGGPKLRLVAAGGTIFLRRSR"
     gene            6892..7773
                     /locus_tag="VN788_11265"
     CDS             6892..7773
                     /locus_tag="VN788_11265"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXU21152.1"
                     /translation="MRFFITFRLCLTSALLLLAAGSANSQTWPGLPAPHVPSVAFAWF
                     GGTSGSYLGIGAVEIGSKRAKSLKLQEDRGVELTCIEDNSPAAKAGLQVGDIVLQYGG
                     EKIKGMEQLSHLVRETPAGRRVKLVIGRDGAKMAISAVIGSRAVTVPSGFRALNTSFP
                     NDDMAAVPFVPGMPGLEQPIGGRNGERIGIEIEALPPQLAGFFGVKAGALVGSVLAQS
                     PGEHAGIRAGDIIVKINGMQIRTPAEVVDAIDSRSSDRIPVLVVRKRRTRILRLNLDS
                     GRPKQPSGVVAEPVNSR"
     gene            complement(7760..8164)
                     /locus_tag="VN788_11270"
     CDS             complement(7760..8164)
                     /locus_tag="VN788_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21153.1"
                     /translation="MNILLSILPYLCIAGGSFACVYLFVSLKKEIYRVRRHVSEKDAG
                     TFEALASFEQKLEALTRELRDIEEIGGGFAPPPAGNGINLTKRTQVLRMVRNGTGEAD
                     IASALHLPRGEVDLLVKVHKLSAGQARKITAN"
     gene            complement(8161..>8376)
                     /gene="fliN"
                     /locus_tag="VN788_11275"
     CDS             complement(8161..>8376)
                     /gene="fliN"
                     /locus_tag="VN788_11275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014450670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HXU21154.1"
                     /translation="VSFGRAQLPLKDVIKLTTGSIIELNRSVSEPVEIIVNNCLIARG
                     EVVVIEGNFGVRIHEIVNRQERLRALK"
BASE COUNT         1787 a         2332 c         2431 g         1826 t
ORIGIN      
        1 atgacgttct gcagttcgcg cacattaccc ggccagtgat actgcatcag cctccggatc
       61 gcgtcgtcgg aaatggaggt caccccgctg tccagatcct tcgcgagttt cttcacgaaa
      121 tactccgcca aaaatggaat atcttcctta tgctcgcgca gcggaggcac attgatcggg
      181 acaacgttta gcctgtagta caagtcctcg cgaaaattgc cttgttcgag cgccgcccgt
      241 aggtccacat ttgttgctgc gatcacccgt acgtcaatct ggcgagtccg gttactgccg
      301 agccgctcga actcacgctc ctgcagaacg cgcagcagct tcacctgcac cgacgccggc
      361 acgtcgccga tctcatcgag aaacactgtg ccggtgtctg cctgctcgaa cttgcccggc
      421 ttgctcgtat tggctcccgt gaacgcgcct ttctcatagc cgaacagctc gctttccatc
      481 agattttccg gaatggccgt gcaattgatc ttcacgaacg gcttgctagc gcgcggagag
      541 tggtgatgaa tcgcgcgagc gatcatatct tttccgacgc cactttcgcc cgccagcaac
      601 acagtcgcgc gcgtcggagc aacgcggtcc accgtcgcga aaatctcctg catcgatggg
      661 ctgtggccga cgagattatc aaactcatag cgcgccccga gctcctcgcg caattttcga
      721 ttctcgtcgc gtagggagcg gatctccagc gccttcgcga ctcgcgccag cagatgttcc
      781 agcgagaacg gtttttcgag aaaatcggcc gcccctgcct tcatcgcttc cacggcagtc
      841 tcaaccgttc catacgccgt catcacgatc accggcgtgt acagattctg ctcacgcaat
      901 ttcgcgagaa attgcaagcc gttcatgccg ggaaggcgaa gatcggtgag aatcacgtcc
      961 gccgtgtcgg cgagccgcgc gccatcttcg gcggttccag cctgtgccac ttcataaccc
     1021 gcacccttca attgcagctc gatcacccgg cgcagtttct cttcgtcttc gatcaccagt
     1081 atgcgagcgc tcatgcctct tccggcctca ccggtagcaa aacacgaaac acgctgcctt
     1141 cgcccttttc gctctcaacg gtgatggccc ccccatgctg atccacgatc tttgaaacga
     1201 ttggaagccc cagcccgacg ccgctcgatc gggtcgtgaa aaatggattg aaaatacttt
     1261 caaggttggc cttatcaatt cctgaaccac ggtcgatcac agctatttct atggaaccgt
     1321 tcagtcgccg ggtcttcacg gtcaccgctc cctgcgccgg actcgcttgc gccgcgttca
     1381 agatcagatt ataaaagacc ctctccatca actcgctgtc gaacggaaaa ggccgcacgt
     1441 ccggggaata gttgcggtag atcactgcgt cgaggggcgg atcgtgccgc tccacctgct
     1501 ggatcgcgcg atcgatcgcc tggtgaacat ccgctagctc cgcgtgcaca gccaatgggc
     1561 gcgcaaagtc caggaaacgt gtcaccagcg aattggtacg gtccacttcg cttgaaatga
     1621 atccggccat ctcgcgcgct atttcgttct cgcccgcaac gctctgcaac agcaattccg
     1681 ccgacgtctt catcgtgtgc agtggattgc gaagctcatg tgccaaaccc gctgaaagtt
     1741 gcccaattgc ggcgaggcgc tccgatcttc ggacggacgc ttccgccact tcgaggttcc
     1801 ggttcgcctc cgccaacttt tcggccgcgg cctgataacg ggcctgcgtg aggctcgatt
     1861 caaccaggtg atgcgtcaac acgctgacca gcagcaagag aagcactcga aggtagagtt
     1921 ccttgatctg gtccggaggg atgtattggt ccggcttaag gcgcaaaagg aatgagagat
     1981 aggcgagaga tgcggccgct gccgccaaaa ttgtctcccc caggttgagg gacgtcgcgg
     2041 ccgagatcac cggaaatagg agtatccagt aatagctgct ggcgacgcca tccgtccaac
     2101 cgatcagcag gtaacagagc accagtttga ttagaaacga gatcgcgctt cccgtttgcg
     2161 tcaaaaacca gggaattttc gattcagcca cctggtagat ggcaagactg aagagcagcg
     2221 cgctcatcat cgtcgtgcga tccggcccaa acagcgcgag cgcggaaaat aacacgatcc
     2281 ataacagatc gcgtacgcga atcaatcgga tcaacggccg cggccaagcg agcggttcca
     2341 cgctgccatg ttatccggga ttgtgccaga atagggtcac aactgctttt cgaatgaatt
     2401 cttccaatgt ttccgcgttt cctctggaca gcgacgttcc tccatcgggg gatgtttctt
     2461 tcggctcgat cctctctcaa tttgaagagg gacagcacga taagcccggc gaaggtgttc
     2521 gtggaactgt ggtctctctt ggtcccgatt ccgttttcgt cgatataggc cggaaaatgg
     2581 acggcgttct gcccatcgaa aaagttcgtg acgagaacgg cgcgattagt gtccgcgttg
     2641 gcgatgagtt ggtggtcagt atcaccggac gcgatcagga aggaaactac cttctttcca
     2701 ctctcgaagt agcgcgcccg aaggattgga gcgcatttga aaaagcgttt gccgataaga
     2761 cgccaatcgc cggcacagtg acggaactgg tgaaaggcgg actgcgcgtc gattgcggcg
     2821 tcaaggcatt cctgccggct tcacggagcg gcgcgcgcga tcaggcggaa atcgaaaagc
     2881 tggtcggtca ggagatccag tgccggatta tcaagctcga catcgccgat gaggatgtcg
     2941 tcgtcgaccg ccgtgtgatt ctggaagaag aagaacgaaa ggccaaggac cgggcgttcg
     3001 acacgcttca ggaaggggcg gtccttcgtg gcgtagtgcg cagcctgacg gatttcggag
     3061 cgttcgtaga ccttggcgga gtagacggcc tgctgcatgt cgctgatatg tcatggacgc
     3121 gcgttacaaa accgtccgac gtcgtgtctc ccggtgactc cattgaagta aaagtgctga
     3181 aagtgagccc ggaaaaccgg cgcgtttcgc tcggtttgaa gcaacttgcc gcggatccct
     3241 ggaccatcgc gttggaaaaa ttaaaagtag gcgatcgtgt tcagggcaag gtttcccggc
     3301 tcacggattt tggtgcattc gtcgagctga tgcccggcgt ggagggcctg atccatatct
     3361 cggagatgtc gtggaccaag aaaatccgca aacctgccga catagtgaat ccggatgacc
     3421 ttgtcgacgt cgtcatactc ggcgtgaaac cggccgagca tcgcatttcg ctaggattga
     3481 aacaagctct cggcgaccca tgggaggaag cggaaagaaa attcgctcca ggaactgtca
     3541 gcgaggggcc tgttacaagc cttaccaact tcggcgcctt tgtggatctc ggcaacggta
     3601 ttgaggggat gatccatatc tcggatatca gcggcaagaa gcgtctgaac catccacggg
     3661 aggcgctgac tgtcggcgag acggtaaagg cggctgtact tgaaatggac aaggcgaagc
     3721 gccgtatccg gctcagcata aagcggctcg agcctacgag cttcgacgag tacgccgccg
     3781 agcacaagat tggcgacgtg gtgaccggac gcgtcattga ggcttcggcc acgaaggcgc
     3841 gggtggaatt gggcgaaggc gtatcagccg attgtagctt gactgcccca gccccgaatg
     3901 cgccgaaaag ctccgcagtc aagccggact tgggtgcggt gacagctctt ttgacagcaa
     3961 aatggaaatc aggcggggga tctcaacagc agttgagcgg tagtggcgtc cgtcagggcc
     4021 aagttcagag tttcaggatc ctcagcctgg acgcgcccca taagcgcatc gaactcgaat
     4081 tggccgaata atcggggtag tttcggcctg gagacgatcc gggtgacctt tcccgacaag
     4141 caccgtctat ctcaattagt gcaagggaat tcgaacgccg tagcggtaca accggagcgt
     4201 tcggttggtt ccatggatga agcggctctg attcgggcgg ctcagcaggg ggatgagtct
     4261 gcattcgagc agttggtgcg tattcacgac caaaacgtgc ttcggcttgc cctcaatctg
     4321 ctccgttcgc aagaagatgc acgcgacgtg taccaggaag ccttcctgcg cgtttaccgc
     4381 aatctcaatg cattccgttt cgactgcagc tttcacactt ggctataccg gattgtgacc
     4441 aatctatgcg tcgacgctct tcgcaagagg cgggtcaggc gcgaagagtc gtccgtgctc
     4501 gccacccccg acggaatgct ggaccgcatg gatacggttg aagaggcgcg tgcggatggc
     4561 gatccgcagc gtcatctgct aagccggcaa ctgaacggta gaattcaatt aacgctcgaa
     4621 tctctgacgc ctcgggagcg aattgttttt gagatgaggc actatcaggg cttgcggcta
     4681 cgagcgatcg gcgatgccct cggcaccacc gaagaagcgg ccaagaattg cttatttcgc
     4741 gcgacgcaga agatgcgcgc cgcgctcgga gattttctat gaggtgcgat gaagttcgga
     4801 agatcgctcc gctatttctt tacggcgagc tttcgtttga tggagaggaa cagttcgaac
     4861 agcacctgga aggttgccca gattgccgga gatttctgga gcgtgaaaaa gctcttcaga
     4921 gttgtctcga tgaagtggag tccgatttac cggcaggact gctcgggacc tgccgccgag
     4981 accttcgggc cagccttgag agttcaagcg ggcaccctgg gcattgggag aggtggcgcg
     5041 aggcgtttgc aattcgatgg attcccttgc cttcctttgc gcagcccgct ggcgcgatcg
     5101 cgctggttct gtttggcttt ttcgccgcgc gcttctattt tggttccatc tctcctggat
     5161 caagcgacgc ccagcatgct gccttgaccg cgccactctc gacacggata agatacttag
     5221 agcccgacgg gtccgggcac gtgcaaattg tggtggatga gacgcgccag agggttttga
     5281 ctggcgatgc ggatgacgaa tcgattcgcc gtcttctttt gacggcggca caggatccgc
     5341 gcaatccggg cctgcgggtt gaatcggttg atttgcttaa aaatcacatg gaatcggccg
     5401 aggtccggcg agctttgctc accgcgcttc agcatgaccc aaacgcggga gtccgcctga
     5461 aggcgctcga tggccttaag cagttcgccg cagatccgga aacgcggcaa gttctcatgc
     5521 atgttttgct cgcggataag aatccgggag tacggacaca agtcatcgat ctgttggtgc
     5581 ggcagaagaa cgaaaagatg gtgggtgtgt tccaagaact gatgcacaag gagcaaaacg
     5641 gctacgttcg cctccgctgt gagcgggctc ttcgtgaaat gaatgcgtcg ttggggacgt
     5701 tctgacgtga taggccaccg tgtgatgcca gccttggccg tgggattact cactggggtt
     5761 ttgagcttcg gacagtcctc caccgctgtc gttcgcgatg gctcgtggtg gactcagact
     5821 tctaaaggat ccattgcggc gtctggcgtg gagcgtgtgc ggattcacgc cgagggcggc
     5881 gtggccctgc tcggaaatag cggcacatcg gtctcgtact ccctcaagga gcgggtacgc
     5941 gcctcgaatt ccgcgcgcgc agagtcccgt ttgcgcgcat tccggataga aagccgccga
     6001 gtaggcgccg cgcttcatct cacattcacg accccaacgg ccggatatac cagcgcggaa
     6061 ctcacggtcg ccgttccacg tgatcttccg atcgttgaaa tcgagagcct gggtggggat
     6121 gcgtcagcga cgaatttcga tggtattctt cggctcgtaa caggcggcgg ccaaatcgat
     6181 gcagaccgtc tccagaattg tttcttcaaa accgctggtg gagatatccg catcggatta
     6241 gttgcgggtc cccttcagtg ttatacaggt ggcggcacga tccacgttag cagagcgagc
     6301 ggtgatgctc atctggaaac ggccggcggc gatatcgacg tgggagaaat tgacggaggg
     6361 cttactgctc tgaccggagg gggtaatatc cacattacac gctcgctcca ggcggtcgtc
     6421 gctaaaacac gaggcggcct gatcgaagtg cagcatgccg ggggccttgt cacggcgcag
     6481 actgccgcgg gttccattca ggtgacctcg tcccagggag ttcattgcgc ttcaacgaac
     6541 ggaacaatca ggctgcggaa cgtggcggga gttttgcggg cttcgaccga atcgggcagc
     6601 atcctggcag aattgcggcc gggagaatcg ctgggggatt caacgctgag cactaatgca
     6661 ggcgatatta cagttttgat accgcctcat atggcgttga ctgtttttgc gcggaatgaa
     6721 tcgcgaggcg cgactgggcg gatcatttcg gattttccgg agattcattc agcggcctat
     6781 catggcttgt tgccacaaac agcccaggga tcgctaaatg gcggcggccc gaagttacga
     6841 ctcgtcgccg cgggcggcac gatttttctt cgtcgctcgc gataacttta tatgcgtttt
     6901 ttcatcactt ttcgtctttg tctcacctca gctttgctgc tcctggccgc cgggtctgct
     6961 aatagccaga cgtggcctgg gctgccggct cctcacgttc cgtcagtggc attcgcgtgg
     7021 tttggcggca ctagcgggag ttatctcggt attggggccg ttgaaatcgg ctcgaaacga
     7081 gccaagtcac tgaaattgca agaagatcgc ggagtcgaat tgacctgcat tgaagataac
     7141 agccctgctg ccaaggccgg cctccaagtg ggcgatatcg tgctgcagta cggcggagaa
     7201 aagatcaagg ggatggaaca actcagtcat ctggtccgtg aaactccggc gggccggcgc
     7261 gtgaagttgg ttatcggtcg cgacggcgcg aagatggcga tttccgctgt catcggttcc
     7321 cgggccgtaa cggtacccag cggtttccgg gcacttaata cgtcatttcc aaacgacgat
     7381 atggcggcag ttccgtttgt gccgggaatg cctgggcttg aacagccgat tggcggccgg
     7441 aacggagagc ggatcggcat cgaaatcgaa gcgttgcccc cgcaacttgc cgggtttttc
     7501 ggagtcaaag ctggagcgtt ggttggctcc gttctggcgc aatctccggg agaacacgct
     7561 gggattcgtg ccggcgacat aatcgtcaag attaacggga tgcaaattag aacaccggcc
     7621 gaagtagttg acgcaattga ttcgcgttcc agcgatagaa tcccggtgtt ggtagtgcgc
     7681 aagcgccgca ccaggatact caggctcaac ctggatagcg gtcggccaaa acagccgtcc
     7741 ggcgttgttg ccgagccggt caattcgcgg taatcttgcg ggcttggcct gcagaaagtt
     7801 tatgtacttt aacgagcaaa tcgacctctc cgcgcggaag atgcaacgcc gaggcgatat
     7861 cagcctcgcc ggtgccgttt cgaaccatac ggaggacttg ggttcgtttc gttaaattaa
     7921 tgccgtttcc ggctggaggc ggagcaaaac ctccccctat ctcctcaata tccctcaatt
     7981 cccgcgtcaa cgcctccagt ttttgctcaa acgaagccaa tgcctcaaag gtcccagcat
     8041 ctttttcact cacatgccgt cgaacccggt atatctcttt ctttaatgac acaaaaaggt
     8101 acacgcaggc gaaagagccc ccggcgatac acaaataagg caaaattgat aatagaatgt
     8161 tcactttaac gctcgcaatc tttcctggcg attaactatc tcgtgaattc gcacaccgaa
     8221 attaccttcg atgaccacaa cttctcctcg tgcaatgagg caattgttca cgataatctc
     8281 gaccggttct gaaacggacc tgttcagttc gataatcgat ccggtggtca gcttgatgac
     8341 atccttcaac ggtaattgcg ccctgccgaa tgagac
//
LOCUS       DATNDT010000350         2350 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3814328, whole genome shotgun sequence.
ACCESSION   DATNDT010000350 DATNDT010000000
VERSION     DATNDT010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2350
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3814328"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1300)
                     /locus_tag="VN788_11280"
     CDS             complement(<1..1300)
                     /locus_tag="VN788_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU21155.1"
                     /translation="MSWVHRAINAVWRFLRWKQAEEELNEEVRAYFEISVAREMERGL
                     SREEAQRVIRMRFEGPEQVKHRVRQSRAGARVEGVFQDLLYAWRTLKRRPGYAVTAIG
                     TLALGIGANTVIFSVVSGVLLRPLPFSHPDRLVQLNESDPRNGASAVSYPDLKDWRKQ
                     SSSFKAMIAYGNVSKDLQTEGEPERIPTVWSERGLFRMLGVEPILGRTFRDDDPLNVV
                     VLSASLWRGRFGGEPSCIGRKLILDGEPYTVIGVMPEGFQFPYRSSHTQLWIPWEAPS
                     EYAQNRNYRIDFVAARLKDKVPIQAAKRELAVIAKRLEAEYPETNKGRAASITPLSEV
                     VTGQVRPALLTLLGAVGMVLLIACANVMNLLLARTATRTHEIAVRSALGASRGRLIQQ
                     LLTESILVSAAGGVAGLLLALVGSPLLLKLASTRIPRFEEI"
     gene            complement(1306..1638)
                     /locus_tag="VN788_11285"
     CDS             complement(1306..1638)
                     /locus_tag="VN788_11285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXU21156.1"
                     /translation="MAKNAELLQGTLDLLILRTLELRPLHGVGIADRIEQVTKGVFVI
                     GPGSLFPALHRLSEKGWIQGEWGELESGHRAKFYTLTQSGRAQLAKEKRNWQKVWTAV
                     HQVLAEEG"
     gene            1726..>2350
                     /locus_tag="VN788_11290"
     CDS             1726..>2350
                     /locus_tag="VN788_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HXU21157.1"
                     /translation="MTIGRRDFLKASAGVAAMLTPHALLFGQNDQAVIDVFPEQGLGT
                     ISRNIYGHFTEHIGGVIYDGVWVGENSKIPNTGGIRKAITDGLRRIHAPVIRWPGGCF
                     ADSYDWRDGIGPAARRPTRTNFWEVTPDAKRLKEKGAQLFESNAFGTDEFVRFCKLSG
                     AEPYLAGNLRSLPALALDQWVEYCNSPAGSTTYARMREAGGSREPFGV"
BASE COUNT          529 a          704 c          638 g          479 t
ORIGIN      
        1 cgatctcttc aaaacgggga atgcgcgtcg atgctaactt caggagaaga ggcgagccaa
       61 caagcgcgag aagcaagccg gcaacgccgc cggcagcgct caccagaata ctttcagtaa
      121 gaagttgctg gatcaaccgt cccctgctgg cgcccagcgc ggagcggaca gcaatctcgt
      181 gtgttctcgt agccgtacgc gccaatagca gattcataac gttggcgcac gcaattaaga
      241 gcaccatccc gactgcgccc agaagcgtaa gcaaagcagg ccggacttgt cccgtcacaa
      301 cttccgaaag cggtgtgatc gatgcggcgc ggcccttatt tgtttcggga tactcagcct
      361 ccagacgctt ggcaataacc gctagctctc tcttcgccgc ctggattggt accttatctt
      421 tcaaccgcgc cgcgacgaaa tcaatgcgat agttccgatt ttgcgcatat tccgacggcg
      481 cttcccaggg aatccataac tgagtgtgcg aactgcgata aggaaactga aaaccttccg
      541 gcattacgcc gataacggtg tatggttcgc catctaaaat cagctttcga ccgatgcacg
      601 acggctcacc cccgaagcgg cccctccaaa gacttgcact caaaacgact acattcagag
      661 ggtcatcatc gcggaatgtc cgccccagaa taggctcgac gcccagcatt cgaaataagc
      721 cgcgttccga ccagacagtg ggaattcgtt ccggctcgcc ttcagtctga agatccttac
      781 tcacattccc ataggcgatc attgccttaa aagacgagct ctgtttacgc cagtccttga
      841 gatcgggata ggacaccgca ctcgcgccat tcctcgggtc ggattcgttt aactgaacca
      901 gacggtcggg atgggagaaa ggcagcggcc gtaaaagcac gccgctgacc acgctgaaaa
      961 tgacggtgtt ggccccaatg ccaagagcca gcgtgccaat cgcggtaacg gcataaccgg
     1021 gcctcctctt caacgttctc caggcataaa gcaaatcctg aaataccccc tctacccgag
     1081 cgcctgcccg cgactgccgg acccggtgct tcacttgctc ggggccctca aagcgcattc
     1141 gtataactcg ttgcgcctct tcgcgggaaa gacctctttc catttcacgc gcaacggaga
     1201 tctcgaaata cgcccgcact tcttcattga gctcttcttc tgcctgcttc cagcgcagaa
     1261 atcgccagac ggcattgatt gctcgatgaa cccacgacac gggctttatc cctcctcggc
     1321 gaggacctgg tgtacggccg tccaaacctt ctgccagttt cgcttttctt tagccaattg
     1381 agcccggccc gattgcgtca gcgtgtagaa cttagcgcga tgtccactct ctaattcgcc
     1441 ccactcccct tgaatccaac ccttctcgga caaccggtga agcgccggga aaagagagcc
     1501 cggtcctatc acaaagacac ctttggtcac ttgctcgatt ctgtccgcga ttcccacgcc
     1561 atgcagcgga cgaagctcta gcgttcgtag aatcagcaaa tccagcgttc cctgcagaag
     1621 ttcggcgttc ttggccatcg cttggagtat atcggatgtc gatatcggcg gtcaatacta
     1681 aagcggcttt tccacgatag tgtaaattat ctctacaaga tggacatgac gatcggaaga
     1741 cgggacttcc tgaaagcctc ggccggagtg gctgcgatgc tcactccgca cgcgctgctc
     1801 ttcgggcaaa atgaccaggc ggtgatcgat gttttccccg agcaagggct gggaacgatt
     1861 tcgcgcaata tctatggcca tttcaccgag catatcggcg gagtgattta cgacggcgtg
     1921 tgggtgggcg agaactcgaa aatcccgaac acgggaggca tacgaaaggc gattacggac
     1981 gggctgcgtc gcatccatgc gcccgtgatc cgctggccgg gcggatgttt cgccgatagt
     2041 tacgattgga gagacggcat tgggcccgcg gcgcggcggc ccacgcgcac caatttctgg
     2101 gaagtgacgc cggatgcgaa gcgcctgaaa gaaaagggcg cgcagttatt cgagagcaac
     2161 gccttcggca cggatgaatt cgtgcggttc tgcaagctaa gcggagccga gccgtacttg
     2221 gcggggaatc tgcgtagcct tccggcgctc gcgctcgatc aatgggtgga gtactgcaac
     2281 tcgccggcgg gatcgaccac ctacgcgcgg atgcgcgaag ccggaggatc gcgggaaccc
     2341 ttcggagtac
//
LOCUS       DATNDT010000351         3237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3525692, whole genome shotgun sequence.
ACCESSION   DATNDT010000351 DATNDT010000000
VERSION     DATNDT010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3525692"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VN788_11295"
     CDS             complement(<1..493)
                     /locus_tag="VN788_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HXU21158.1"
                     /translation="MADTTPEIDMDTPVMTIESLLSRQRPRRNVQGIAAALLPFEADG
                     KIAVESFQNHLRATHRAGLMNAVNMDTGYVNYLSDAEKRDVLRWTRDGLGEGVPFVAG
                     AYIEGQSGEIVDLYRVQIEPIVRAGGIPILFQTARLHGKSPKEKADTYREICEGYPHV
                     LAFE"
     gene            complement(515..1975)
                     /locus_tag="VN788_11300"
     CDS             complement(515..1975)
                     /locus_tag="VN788_11300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007413064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HXU21159.1"
                     /translation="MKKPILRVASIKDTARFQEHLASLNVAIPCDSNLARPEDSPLRW
                     PLTKGGIKIGNRIAVQPMEGWDGLPDGNPSENTIRRWKRFGSSGAKLIWGGEAVAVVH
                     DGRANPNQLVAAPHTEAGIARLRDTLIAEHRSRAGSTDGLLIGLQLTHSGRYCRPNVH
                     DRPEPKILYHHPFLDRRLRLPADCPVLSDAEIQGIIEKFHCAAVMAHKLGFDFVDIKH
                     CHGYLGHEFLSARTREGRYGGSFENRTRFLREVAQGIRSLAPGLQIGVRLSAFDTIPF
                     RPDPQQSVNGKLGPGIPEICESGNGYTFGFGTDASNPVDIDLEEPTKFLSLLEELGIY
                     LVNLTAGSPYYNPHIIRPALFPPSDGYLPPEDPLVGVARQMNVVRTLKQRHPNLIFMG
                     SAYTYLQDFLPNVAQAALREGWVDCVGIGRMILTYPEILWDAAEGREVARKHICRTFS
                     DCTTAPRNGMPSGCYPLDDYYKQSEYASRVAQAKKH"
     gene            complement(1972..>3237)
                     /locus_tag="VN788_11305"
     CDS             complement(1972..>3237)
                     /locus_tag="VN788_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013766130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU21160.1"
                     /translation="DVDEPKHSASISQPKEVALEAPVLNSQAPPQSGGIYAATTAFVV
                     LFCIVGLALWGLPFYYDFMVRQFGWTRAQVTSGNAISKLVIGPAFGFFAGWIIDRFGP
                     RLMMIAGILMAGAALVGLGWVSSLGMFYLFYFFNALGYVCGGPLPNQVLLTRWFDKGR
                     GKAMGFAYLGIGFGGAAVPWIANALITRFGWQASLRYLGIGIVVVSLPLAFFIKEIPA
                     PVKSEPRAAPVSVKAAFKSGAFYLLTLGSMCSIAAVSGTQQNLKLFLSLDRHFSQTAA
                     ARVLSLILTFSILGRLLIGWLADRFSKKHVVLLTYFLVAAGIPLLFLGRSAIALYVSA
                     AVFGTGLGGDYMVIPLITAEIFSGQMLGRLLGVILTAGGMAEAVSPWMMGYLRDTTGS
                     YLASCVVLVIVAVLGMIAVLGLPDGQKAT"
BASE COUNT          764 a          947 c          839 g          687 t
ORIGIN      
        1 attcaaatgc cagcacgtgc ggatagcctt cgcaaatctc tcgatatgta tctgcttttt
       61 ccttcggcga tttcccatgt aagcgcgccg tttgaaagag aatgggaatg ccgcctgcgc
      121 ggacaatcgg ctcgatttgt acgcgataca aatcaacgat ctcgcctgac tgcccttcaa
      181 tataggcacc cgcaacaaat ggaacgccct ctccgagccc atcacgcgtc cagcgcaata
      241 cgtcgcgctt ttccgcatcg ctcagatagt tcacgtatcc ggtatccatg ttcacggcgt
      301 tcatcaggcc tgcacgatgt gtcgcgcgca gatgattctg aaacgattcc actgcgatct
      361 ttccgtcggc ctcaaacggc aggagcgccg cagcaatacc ctgcacgttt cgacgaggcc
      421 tttgccggga gagcagagat tcaattgtca tcacgggagt gtccatgtcg atttccggcg
      481 ttgtgtccgc catttctcgc ctttgaagcg tgtgctaatg tttctttgct tgagccacgc
      541 gtgaggcata ttcagactgt ttgtagtaat cgtcaagtgg gtagcagccc gaaggcatgc
      601 cgttgcgcgg agccgtcgtg caatcgctga aggtcctgca gatatgcttt cgtgcgactt
      661 cgcgaccttc cgcggcatcc cataagattt ccggatacgt caggatcatg cgaccgatgc
      721 caacgcagtc gacccatcct tcacgcaagg ccgcctgggc cacgttcggc aggaaatctt
      781 gcaggtacgt ataagccgat cccataaaga tcaaattcgg gtgccgttgc ttgagtgtcc
      841 tgaccacgtt catttgccgc gccacgccaa ccaaaggatc ttcaggcggc aaatatccat
      901 ccgacggggg aaagagcgcg ggtcggatga tatgcggatt gtagtatggg cttcctgcgg
      961 ttagattaac gagataaatg ccgagttctt ccagcagtga gaggaatttc gtcggctctt
     1021 ccagatcgat gtcgacggga ttcgatgcat cggtgccaaa tccgaacgtg tagccattgc
     1081 cgctctcgca gatttccgga attccgggcc cgagctttcc attaacgctc tgttggggat
     1141 cggggcgaaa cggaatcgta tcgaacgctg aaagtcgcac tccgatttga agaccgggcg
     1201 cgagcgaacg aataccctgc gctacctcgc gcagaaaacg cgtgcggttt tcgaagctgc
     1261 cgccgtagcg cccctcgcgc gttcgcgcac tcagaaattc gtgccccaga taaccgtgac
     1321 agtgcttgat atccacgaaa tcgaagccga gcttatgcgc cattacggcc gcgcaatgaa
     1381 acttttctat gatgccttga atctccgcgt cgctcagtac cggacaatcg gcgggcaagc
     1441 gcaatcgacg gtcaagaaag ggatggtgat acagaatctt cggctcgggc cgatcatgca
     1501 cattcgggcg gcaatagcgg cccgaatggg ttaattgcaa gccgatcaga aggccatctg
     1561 tcgagcctgc ccgcgagcga tgctccgcga tcaaggtgtc gcgcaaccgt gcaattccgg
     1621 cttccgtgtg cggcgctgct accagttgat tcgggtttgc gcgcccatca tggacaacgg
     1681 ccacggcttc gccgccccaa atcagctttg ctccgctaga accaaaacgc ttccagcgac
     1741 gaatcgtatt ttcgctggga ttaccatcgg gtaagccgtc ccagccttcc attggctgaa
     1801 cggcaatgcg atttccgatt ttgatcccgc ccttagtgag cggccatcgc aatggcgaat
     1861 cttctggccg ggccaggttg ctatcgcagg gaatcgctac attgagcgaa gcgaggtgtt
     1921 cctgaaaccg cgccgtatcc tttatgctcg ctacgcggag aatgggcttt ttcatgtcgc
     1981 cttttgtccg tccggcagtc ccaatactgc aatcatccca agcaccgcca caatcacaag
     2041 tacaacgcag cttgccaggt aacttccggt tgtgtcacga agatatccca tcatccatgg
     2101 tgacacggct tccgccattc cgccggccgt cagtatcacg ccaagaagcc tgccaagcat
     2161 ctggccgcta aagatttccg cggtgatcag cggaatcacc atgtagtcgc cgccaaggcc
     2221 cgtcccgaat accgcagcgg aaacgtagag tgcaatcgcg cttcgtccca ggaagagaag
     2281 cggaataccg gcagcgacca aaaaataagt taagagcact acatgctttt ttgaaaaccg
     2341 gtcggcgagc catccaatta gaaggcgtcc caaaatgcta aaggtgagaa tcagcgatag
     2401 aacgcgtgcc gctgccgtct gcgaaaagtg ccgatccagg ctcagaaaga gcttaagatt
     2461 ctgctgagta ccgcttacgg ctgcaatcga acacatactc cccaacgtta ggagataaaa
     2521 tgcgccgctt ttgaaagcgg ccttcacgct cacaggagcg gcacgaggtt cagattttac
     2581 cggcgccggg atttctttaa tgaaaaacgc cagtggaagt gaaacgacga caatgccgat
     2641 tcccagatac cttaacgaag cttgccatcc gaatcttgta atcaaggcgt tggcaatcca
     2701 tggcacggcc gctcccccaa agccgattcc cagatatgcg aaccccatag ccttgccgcg
     2761 gcccttatca aaccaacgcg tgagcagcac ctgatttggc agagggcctc cgcagacgta
     2821 acccagcgcg ttaaaaaaat aaaaaagata aaacataccc aaactcgaga cccatccgag
     2881 tcccaccagc gctgcgcccg ccatcaaaat tcccgctatc atcatcagtc gcggaccgaa
     2941 ccggtcgatg atccatcctg caaaaaagcc gaatgccggc ccgatcacga gcttgctgat
     3001 cgcattccct gatgtcacct gtgcgcgcgt ccagccgaac tgacgcacca tgaaatcgta
     3061 ataaaaggga agtccccaca gcgccaagcc cacaatgcaa aagagcacta cgaatgcggt
     3121 tgtcgcggca taaattccgc cgctctgcgg tggcgcttgt gagttgagaa cgggagcctc
     3181 aagcgcaacc tccttaggct gcgaaatact tgcagagtgc tttggttcgt ccacgtc
//
LOCUS       DATNDT010000352         6399 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4214696, whole genome shotgun sequence.
ACCESSION   DATNDT010000352 DATNDT010000000
VERSION     DATNDT010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6399
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4214696"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VN788_11310"
     CDS             complement(<1..866)
                     /locus_tag="VN788_11310"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXU21161.1"
                     /translation="MSKRLSLISSAIIAVSLFSLALFVAPPLAAAQTSTQSSSASPEL
                     LSKVESYLRNLFGWGPEYKINLGPVTSAEIPDLLKIPVSVDFQGNNQKGTVYVTKDAH
                     FMFRGEVRDMTKNPFADNLAKITTANSPSKGPADAKLTVVEFSDFECPHCQEAHNILL
                     QIEPEFPQVRWVFKNFPLAQIHPWAMSAALAGRCVYKTSNDAAFLKFQDSVFTNQSAI
                     TPDNAWDMLTGYAVAAGASADALHSCMTDPSTKSAVEADLAEGKSLEVESTPTFFING
                     RPFQPSDKASFE"
     gene            complement(1018..1353)
                     /locus_tag="VN788_11315"
     CDS             complement(1018..1353)
                     /locus_tag="VN788_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS domain-containing protein"
                     /protein_id="HXU21162.1"
                     /translation="MFIFQYNLFNQVPVKFPGRETDSKPERIDIIARDKNGSMVVIEL
                     KAGKAGRRAIGQILGYIGSLMARRQPVRGILIAQRFSRHAIAAARPVPTLRLRLISNL
                     RTRTRKGSR"
     gene            1531..1962
                     /locus_tag="VN788_11320"
     CDS             1531..1962
                     /locus_tag="VN788_11320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21163.1"
                     /translation="MSRIIAQLMIVVFVAAVSSVSVSAQGTAKPMVWTDPTNQYANYL
                     EAAVLKKHTPVTFTTEKASASYIATLDAEAQKGSAARAIFLGAANSGAKSNLSLTVSD
                     AKTGAVVFSYTCQKNGEFGSFQSASECLAKHWTHFMAKGKP"
     gene            complement(2299..2838)
                     /locus_tag="VN788_11325"
     CDS             complement(2299..2838)
                     /locus_tag="VN788_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU21164.1"
                     /translation="MKLDLEHAIALLARTPAALDALVRGLPDAWTLANEGERTWSAFD
                     IVGHLIHGERTDWMPRAKIILQFGEARAFTPFDRLAQQRESHGKSLPQLLDEFAALRE
                     ENLRELRSLDLQACDLDRRGKHPSLGTVTLSQLLAAWAVHDLTHLHQMSRVLAHQYRD
                     AVGPWSAYLGVLHCAGHSS"
     gene            complement(2880..4517)
                     /locus_tag="VN788_11330"
     CDS             complement(2880..4517)
                     /locus_tag="VN788_11330"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014785391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXU21165.1"
                     /translation="MKIALAQFNPTVGDFLGNSARMLEMAADAKSRAADLVIFSELCL
                     CGYPPQDLVERPAFLERNQTALRELAAKMPLPAIVGYVGKAQVQPGKPAANCAALLRN
                     GEIAFRQRKMLLPTYDVFDETRYFQPARSQSNVELAGQQLGVTICEDAWNDKDYWAQR
                     LYDRDPVAELIGQGTNILINISASPYNLDKRNLRADMLRSLARHHKIPIVYVNQVGGN
                     DSLVFDGSSVGFDAEGNLVARAHSFEEDLVIFDTASNTGDIRPQLTDELEAAYTALVV
                     GTRDYVRKCGFREVVIGLSGGIDSALVAAIATDALGKENVLGVSMPGPYSSKGALVDA
                     AQLAQNLDIRYRVLPIEDVFQAYRKTLCDIFKGQKEDVCEENIQARIRGNLLMALSNK
                     FGAMVLSTGNKSEYAVGYSTLYGDMAGGLAVISDVPKSMVWELARFVNRDRERIPDSS
                     ITKIPSAELRPNQTDQDTLPPYDILDRILKAYVEDLRSPDEIAAHYDFPIELVRDVAR
                     MVDRSEYKRKQSPPGLKITSKAFSVGRRFPLANKFLP"
     gene            complement(4582..4842)
                     /locus_tag="VN788_11335"
     CDS             complement(4582..4842)
                     /locus_tag="VN788_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21166.1"
                     /translation="MTGVAEKRENIFENRLLKLAIVMLVFPVLVLAFHELLVLPALIV
                     GYLLMHFAGHPKVIGETLGLLTMIPACWGAVVMCKWIWRGSK"
     gene            complement(5080..5406)
                     /gene="erpA"
                     /locus_tag="VN788_11340"
     CDS             complement(5080..5406)
                     /gene="erpA"
                     /locus_tag="VN788_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HXU21167.1"
                     /translation="MVSLTPVAVTKVKEIISQQNPVPTGLRVAVVGGGCSGFSYHMAF
                     ESQVNEGADNVYEFDGLKVLVDQMSEMYLDGVSIDYIESLEGSGFKFNNPNVKSTCGC
                     GSSFSV"
     gene            complement(5552..>6399)
                     /locus_tag="VN788_11345"
     CDS             complement(5552..>6399)
                     /locus_tag="VN788_11345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21168.1"
                     /translation="PRPRAGRWTYIVLILSLAFVLMPFLFWRATWFGRPLTNSQLAEY
                     LNPASNPHDVQHALSQVADRIIAGDASVKSFYPQVIALAANPHPEIRAMSAWAMGQDN
                     QSVPFHSALAPMLADSDVNVRRNAALALVRFQDAAGHDIIVNMLRPTAINSPYSGTLR
                     ARIKSGESINPGDLLAKIATPSGTKDLRSEIPGTFNRWLANDGSTISAGQPVANLLPS
                     DDEVWESLRALYLIGTPADLDAIAPYARGLDGLSPKIAEQARQTMSQIRSRPSTESSA
                     NPATD"
BASE COUNT         1414 a         1486 c         2173 g         1326 t
ORIGIN      
        1 tgttcgaagg aggctttgtc ggagggctgg aaggggcgtc cgttgatgaa aaaagtgggc
       61 gtgctttcga cttcgaggga tttgccctcg gcaaggtcgg cttcgacggc cgatttggtg
      121 gagggatcgg tcatgcatga atggagcgcg tcggcggaag cacccgccgc gacggcgtag
      181 ccggtgagca tgtcccaggc gttgtcgggc gtgatggcgg actgattggt gaaaacagaa
      241 tcctgaaatt tgagaaaggc ggcatcgttg gaggttttgt agacgcagcg tcccgcgagc
      301 gcggcggaca tggcccaggg atgaatctgt gcaagcggga aatttttgaa gacccagcgg
      361 acttgcggaa attcgggttc gatctgcagg agaatgttgt gagcttcctg gcaatgcggg
      421 cattcgaagt cgctgaattc gacgacggtg agtttggcgt cggcgggccc ttttgacggg
      481 gaattcgcgg tagtgatttt ggcgagattg tcggcaaagg gatttttggt catgtcgcgg
      541 acttcgccgc gaaacatgaa gtgcgcgtct ttggtaacgt agacagttcc cttctgattg
      601 ttgccctgaa aatccacgga gacaggaatt ttcaagaggt caggaatttc ggcggacgtg
      661 actgggccga gattgatttt gtattcgggt ccccagccga aaagattgcg gaggtaggat
      721 tcgactttgg agaggagttc gggagaggcg gaggaggatt gcgtggaagt ttgggcggcg
      781 gcgaggggcg gggcgacgaa gagcgctaaa gagaacaatg agacggcaat aattgcggat
      841 gagataagag aaagacgttt ggacattata ggtatacctt tcaaaatgaa aactgtacct
      901 gcattaggga atttgtatgg gccagttccc tgctagatgg cgccgctaca tcgaacgaaa
      961 aggatacatc gtgtttaaag gaggtgacaa gcgcgagcga ccggcggtcc aaaacagtta
     1021 tcgcgaacct ttgcgtgttc ttgttcgcaa attggaaatc agtctgagcc tgagggtggg
     1081 aacaggacgt gcggcagcta tcgcgtgacg agaaaacctc tgagcaatta agatgccgcg
     1141 cacaggttga cggcgcgcca tgagagagcc gatgtacccg agaatctgtc ctatagcacg
     1201 tctaccggcc ttgccggcct tcaattcaat gacgaccatc gagccgttct tatccctcgc
     1261 gataatgtcg atgcgttcgg gcttggagtc ggtttccctt cctgggaatt tgaccgggac
     1321 ttgattgaac aggttgtact gaaaaataaa cacatggaat ggagcggaga ggctcggaag
     1381 ggtgcagacg cttgctgtac gttagtacag cttacggttc aaacatgcct gcgtcggaca
     1441 gcgtgaaaga gtgtgacaga ttcctgcaca atcatttgtg gcgacgaagc acgagtccgc
     1501 caaacacaag ttcaaaaaag gagagaactg atgagcagga tcattgccca gttgatgatc
     1561 gttgtcttcg ttgctgctgt ttcctcggtt tccgtcagtg cacagggcac ggcaaaaccg
     1621 atggtatgga ctgatccgac gaaccagtat gcaaactact tggaggctgc ggtgttgaag
     1681 aagcacacac ccgtgacttt tacgacggag aaagcgtccg cttcatatat tgccactctg
     1741 gatgctgaag cgcagaaagg cagtgcggcg cgcgcgatct tcctgggagc ggcaaatagc
     1801 ggcgcgaaga gcaacttatc cctcactgtg tcggacgcga agacgggcgc ggtcgtgttc
     1861 tcgtatacgt gtcagaaaaa tggggagttt ggcagttttc aaagcgcgtc agaatgcctg
     1921 gcaaagcatt ggacgcactt catggcaaaa ggcaagccct gagatcaggg gttctgcagg
     1981 cgctgcagtc gagagaccct tccacgggcc ccacgctcaa aaaacgagcg tggggcaccc
     2041 tgacgagcca agagtatagc aacgtcggcg gtgagacact gcgctacaaa agccagaatc
     2101 agatccctca catcatttga aaagaacgcg aacgagttag gaatgagaag tggcttacga
     2161 ttccacgact ctgagtggcg gcagcttgtg gcaaagcggc gtcgagccgc ggcactcccg
     2221 ttggaacgtc gcagcgcgca accagaaaag ccgcagaact tcgcgggggc gcgaagctct
     2281 gcgctacaaa cggaaatatc acgagctgtg gccggcgcag tgcaggacac cgaggtaggc
     2341 gctccatggg ccgactgcat cacggtattg atgagccaaa acgcgggaca tttggtgcag
     2401 atgcgtgaga tcgtgaacgg cccatgcggc cagcaattgc gaaagggtta ctgtgccgag
     2461 cgaaggatgc ttcccgcgac ggtccaagtc gcacgcctga agatctagtg aacgcaattc
     2521 gcgaagattt tcctcgcgca gcgctgcaaa ttcgtcgagc aactgcggca gcgatttgcc
     2581 gtgactctcg cgctgttgcg ccaggcgatc gaagggcgtg aacgcgcggg cctcgccgaa
     2641 ttgtagaatg attttggcgc ggggcatcca gtccgtgcgc tcaccgtgaa tgagatgacc
     2701 gacgatatcg aatgcgctcc aagtgcgttc gccctcattc gccagcgtcc atgcgtcggg
     2761 caatccacgc acgagcgcgt ccagcgcggc gggggtgcgg gcgagaagcg cgattgcgtg
     2821 ttcaagatca agtttcattg tgttctccat taatcactct cgcattggta agttgtggct
     2881 tacggaagga atttattggc cagcgggaag cggcggccta cggagaaggc cttggatgtg
     2941 attttgaggc ccggaggcga ttgcttgcgc ttgtattcgc tgcggtcgac catgcgggcg
     3001 acgtcgcgga cgagttcgat gggaaaatcg tagtgggcgg cgatttcgtc aggcgagcgg
     3061 aggtcttcga cgtaggcttt gaggatgcgg tcgagaatgt cgtagggcgg gagcgtgtcc
     3121 tgatcggttt gattgggccg aagttcggcg gaaggaattt ttgtgatgct ggaatcgggg
     3181 atgcgttcgc ggtcgcgatt gacgaagcgc gcgagttccc agaccatgct cttaggcacg
     3241 tcagaaatga cggcgaggcc gccggccatg tcgccgtaaa gagtggagta gccgacggcg
     3301 tattcggatt tgttgccggt actgagaacc atggcgccaa atttgtttga aagcgccatc
     3361 agcaggttgc cgcgaatgcg cgcttgaatg ttttcctcgc agacgtcctc tttttgtcct
     3421 ttgaaaatat cgcagagcgt cttgcgatac gcctgaaata cgtcttcgat gggcaggaca
     3481 cgatagcgaa tgtcgagatt ttgcgcgagc tgcgcggcgt caacgagcgc gcctttcgag
     3541 gaatacggtc cgggcatgga gacgccgagg acgttttctt tgccaagggc gtcggtggcg
     3601 atggcggcga cgagagcgga gtcgatgccg ccgctcaagc cgatgacgac ttcgcggaag
     3661 ccgcatttgc ggacgtaatc gcgcgtgccg acgacgagcg cggtgtaagc ggcttcgagt
     3721 tcgtcggtga gctgcggacg aatgtcgccg gtgttggagg cggtgtcgaa aatgacgaga
     3781 tcttcttcga acgaatgcgc gcgggcgacg agattgcctt cggcgtcgaa gccgacgctg
     3841 gaaccgtcga aaacgagact gtcgttgccg ccgacttgat tgacgtagac gatgggaatt
     3901 ttgtgatggc gcgcgaggga gcggagcatg tcggcgcgga ggttgcgctt gtcgagattg
     3961 tagggcgagg cggagatgtt aatgaggatg ttagttccct gccctatgag ctcggcgacg
     4021 ggatcgcgat cgtagaggcg ctgcgcccaa taatctttgt cgttccaggc gtcttcgcag
     4081 atggtgacgc cgagctgctg gccggcgagt tcgacattgg attgcgagcg cgcgggctgg
     4141 aagtaacggg tttcgtcgaa aacatcgtaa gtggggagaa gcattttgcg ctggcgaaaa
     4201 gcgatttcgc cattgcgaag gagcgcggcg caattcgcgg caggcttgcc gggctggacc
     4261 tgggctttgc caacatagcc gacgatggcg gggagaggca ttttggcggc aagttcgcgg
     4321 agggcggttt ggttgcgttc gaggaaggcg ggacgttcga cgagatcttg cggagggtag
     4381 ccgcagaggc agagttcgga aaagatgacg aggtcggcgg cgcgggattt tgcgtcggcg
     4441 gccatttcga gcatgcgggc ggagttgccg aggaagtcgc cgacggttgg attgaactgg
     4501 gcgagagcga ttttcatgta gaagaatcag gatactaagg gcaggaaccc ttcgcaacta
     4561 cacacaaacg cgggtggtgg gctactttga accgcgccaa atccacttgc acatgactac
     4621 cgcgccccag caggcaggaa tcatggtcaa caaacccaat gtttctccga taactttggg
     4681 atgtcctgcg aaatgcataa gcaagtagcc gacgatgagg gctggcagca caagcagttc
     4741 atgaaaagct aaaacgagga ctgggaaaac gagcattaca atcgcgagtt tcagcaatcg
     4801 attttcaaag atgttttcac gtttctcggc aaccccagtc acgcctcctt tttgtcctcg
     4861 cttcgtaaga ttcccacagg ctcaccaaat ctgcaacgcc aaagcctata agacaagaag
     4921 ggggttcgag ggatgtgacc gcggacggga agatcccccg ggtggacgag gtcaaacgaa
     4981 aaaggcggcg taaagccgcc cctacacaaa aaaggcgctc gggggagccg agcgccgtaa
     5041 aatttacgtc agaagaaact taaagatgcg cggtgtgagt taaacgctga aggaagagcc
     5101 gcagccgcag gtgctcttaa cgttcggatt gttgaacttg aagccggagc cctccaggga
     5161 ctcgatgtaa tcgatggaga cgccgtcgag atacatttcg ctcatctggt cgacgaggac
     5221 tttgaggccg tcgaattcgt aaacgttgtc ggcgccttca ttgacctgac tttcgaaggc
     5281 catgtgatag ctgaagccgg agcagccgcc tccgacgacg gcgacgcgca ggcccgtggg
     5341 gacgggattc tgctgcgaga tgatttcctt gactttggtt acagctaccg gcgttagaga
     5401 aaccataggt tcgttgcctc tcagaataag tctcttaaat cgttcgaaga tattatagca
     5461 aattttgtgt gggcgtgcgg gcctgggctg cgcacatttt attggatgtt gtcctaatgg
     5521 cttggatgcg gtctgcataa atccttacaa ctcaatcggt ggctgggttg gcggatgatt
     5581 ccgttgaggg gcgggagcgg atttgggaca tagtttggcg ggcttgttcg gcgattttgg
     5641 gggagaggcc gtcgaggccg cgggcgtagg gcgcgatggc gtcgaggtcg gcgggcgtgc
     5701 cgatgagata gagagcgcgg agagattccc aaacttcgtc gtcgctgggg aggagattgg
     5761 cgacgggctg accggcggag atcgtcgagc cgtcgttggc gagccagcgg ttaaatgtgc
     5821 cggggatttc agagcgcaga tcttttgtgc cgctgggcgt ggcgattttg gcgaggagat
     5881 cgccgggatt gatggattcg ccggatttga tgcgcgcgcg aagagtgccg gaatagggcg
     5941 agttgatggc cgtggggcgg agcatgttga cgatgatgtc gtggccggct gcgtcctgga
     6001 aacggacgag ggcaagggcg gcgttgcggc ggacgttgac gtcggaatcg gcgagcatgg
     6061 gcgcgagcgc ggagtggaat ggcacggact ggttatcctg gcccatggcc catgcggaca
     6121 tggcgcgaat ctcgggatgc ggattggcgg cgagcgcgat gacttgcggg tagaacgatt
     6181 tcacggaggc gtcgccggcg atgatgcggt cggcgacttg cgagagcgcg tgctggacgt
     6241 catgcgggtt ggaggcggga ttgaggtatt cggcgagctg ggaattggtg agagggcgtc
     6301 cgaaccaggt ggcgcgccag aaaagaaaag gcatgaggac gaaggccagt gagaggatta
     6361 gaactatgta agtccagcgg ccggcgcggg ggcgcggcg
//
LOCUS       DATNDT010000353         3045 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2522292, whole genome shotgun sequence.
ACCESSION   DATNDT010000353 DATNDT010000000
VERSION     DATNDT010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522292"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..1048
                     /locus_tag="VN788_11350"
     CDS             236..1048
                     /locus_tag="VN788_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21169.1"
                     /translation="MPNSAFAQYWRLAFLLFVVFAPFFATADNIPVRFTEGISRGFLV
                     VTDLGGHRIGQGDDEQVLHGSEIHNHLIIRFKDGSLYDETTVFTQQKTFRLISDHLIE
                     KGPSFKTPIDGLIDASTGQVIIHYIGKTKDKAFHKKMKLPPDIANGMLFVLVKDFDPK
                     SLKTTMSYLSLGEHPRLVKLVFTPKGKNPFDTGGVRRDAVHYVMHVDIGGIAGALAPL
                     VGKQPHDTDIWVIGGDAPTYAGSVGPLYGDGPVWRISLVSPEAALEEGDGRR"
     gene            complement(1075..1983)
                     /locus_tag="VN788_11355"
     CDS             complement(1075..1983)
                     /locus_tag="VN788_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HXU21170.1"
                     /translation="MKSLCAIAIAIFAATLAFGQASVTDQSSAASQPASPKLEFEVAS
                     IRPAQSNDTNRVDAGLRMDGAQAHFGALSLKNLIARAYHIQSNLITGPDWISSQRFDI
                     SAKLPEGATTDQIPEMLQSLLAERFGLRIHFEKKDQPAYALIIGKSPLKLKSLPPDAD
                     QQKGNGTVNLAVSGSDQGVSMDLGNGSSYAFANDQFQFTKVTMDVLALRLSLYLDRPV
                     INMTDLKGNYDLTLPVTQEDYYILLVRSGANAGVTLPPRALALLNGTPTSLFDALDAE
                     GLHLDARKLPLDTIVVDQALQTPTEN"
     gene            complement(2209..>3045)
                     /locus_tag="VN788_11360"
     CDS             complement(2209..>3045)
                     /locus_tag="VN788_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU21171.1"
                     /translation="LVQIAAQKKLRNCTAHNLRYYPLVQHMRRMVEAGELGEIIVVQG
                     TYSQDWLLLDTDYNWRIESKENGASRCMADVGSHFCDMTEHVTGKRITEVCADLYTFY
                     KTRRKPKGQIEAFAGKALPPDAYTTYPVDTEDMGTVIFRMDNGARGCYSASQVSAGRK
                     NHLRIEIYGTKAGVAWNQERPDELWIGKRDSANETMIKDPSLLKGDARTFADIPQGHS
                     EGYDDTFKQMLRRFYKSILDPSAKPEYPQFADGLRQLTILEAELDSNKKHGWVDVPKA
                     AH"
BASE COUNT          669 a          771 c          907 g          698 t
ORIGIN      
        1 cgcagtcaga cctgcggctc cagcgaggcg ctgaagaaaa ccgcggcgag gagaaacaat
       61 gtggttcggc atgaggagaa ctcctttcca actctgaaga tagcgacagc tcgcgcatgg
      121 taacacagcc gatcggtcac atatgtgcgt ggatagtgct gcagttttct catgccgaaa
      181 aatagaggct atgccccagt cgcgcgtgaa ggctgattga ataggattga gttgtatgcc
      241 gaattctgcc tttgctcagt actggcgact cgcatttctt cttttcgttg tgttcgcgcc
      301 gtttttcgcg acagctgaca acattcccgt tcgtttcacg gaaggcattt cgcgagggtt
      361 tctggtcgtg accgacctag gcgggcacag gatcggacaa ggtgacgacg agcaggtgct
      421 gcatggcagc gaaatacaca atcatttgat cattcgattt aaggacggtt cgctctacga
      481 tgagacgact gtgttcacgc aacaaaaaac ctttcgattg atcagcgatc acctgattga
      541 aaaaggtccg tccttcaaga cgccaatcga tgggctaatc gacgcctcaa ccggacaggt
      601 catcattcat tacatcggta aaaccaaaga caaagcgttt cataagaaga tgaaactacc
      661 gcccgacatc gccaacggga tgcttttcgt cctcgtgaag gacttcgatc caaagtcgct
      721 taagactacc atgtcgtatt tgtcgcttgg ggagcatccc agactcgtga agctcgtatt
      781 cacgccaaag gggaagaacc cgtttgacac cggcggcgtg cgcagagatg cggtgcatta
      841 cgtcatgcat gtggatattg gcggtatagc tggggccctc gcgccgctgg tcggcaagca
      901 gccgcacgat acggatatat gggtgatcgg cggcgacgcg ccaacgtacg ccggttcggt
      961 ggggccgtta tatggagatg ggccggtgtg gagaatcagc ctggtgagcc cggaggcagc
     1021 gctggaggag ggagacggac ggcggtgagt ggtcaccagg aagccgtgcg tgcactagtt
     1081 ctctgttgga gtttgaaggg cttgatcgac gacgatggtg tcgagtggga gtttgcgggc
     1141 atcgagatgg agaccttcgg cgtcgagtgc gtcgaagagc gaagtcggcg ttccgttgag
     1201 cagcgctaga gctcgcggcg gcaatgtaac gcccgcgttc gcacctgaac ggacgagcag
     1261 aatgtaatag tcctcctgcg tgaccggaag cgtgaggtcg tagttacctt ttaggtctgt
     1321 catattgatg acggggcgat caaggtagag cgaaagccgc agggccaaca cgtccatggt
     1381 gactttcgta aattggaatt gatcgtttgc aaacgcatac gaactcccgt tgcccaagtc
     1441 catggaaacg ccctgatcgc tgccggatac ggcaaggttc actgtgccgt tgcccttctg
     1501 ctgatctgca tcaggcggaa gcgattttaa cttcaatggc gatttgccaa ttatgagcgc
     1561 ataggccggc tggtctttct tttcaaagtg aatcctcagg ccaaacctct ccgcgagcag
     1621 ggattgcaac atttcaggaa tctgatcagt ggtcgcacct tcgggaagct ttgcgctgat
     1681 atcgaagcgt tgggacgaaa tccagtccgg gcctgtaatc aggtttgact gaatatgata
     1741 tgcgcgggca atcagatttt tgaggctgag cgcgccgaag tgggcttgtg cgccatccat
     1801 gcgcagcccg gcatccacac gattcgtatc gttggattgc gcggggcgga tcgaggcaac
     1861 ttcgaattcg agcttgggag acgcaggctg gcttgctgcg ctggattgat ccgtaactga
     1921 ggcttgcccg aacgccaacg ttgcggcaaa gattgcgata gcaatcgcgc agagagattt
     1981 catgtgctgt ctccaaagac tgactgaatg acgcggtctt cagattctaa tacgcttttt
     2041 gggcggcgat gttacagcgc aaacgggcag aagcccgacc accgaaaact gcaccgaaac
     2101 tgacaatggt gccacagcga aatgccatgg caccacgttt aagccttcag actctgcgga
     2161 ttgacacaca aacccaagat ggaaagctgc gacgaccaca gatcattctt agtgcgcggc
     2221 tttgggaacg tcgacccagc cgtgcttctt gttgctatcc aactccgctt caaggatcgt
     2281 taattggcgc aagccgtcgg caaactgcgg gtattcgggc tttgccgaag gatcgagaat
     2341 cgacttgtag aagcggcgga gcatctgctt gaaagtgtcg tcgtatcctt cgctgtgtcc
     2401 ttgcgggata tcggcaaagg tgcgggcgtc gcctttgagc agagacggat ccttgatcat
     2461 tgtctcgttg gccgaatccc gtttgccgat ccagagttcg tctgggcgct cctgattcca
     2521 tgcgacgccg gccttggtgc catagatttc gatcctgagg tggttcttgc ggcctgcgga
     2581 aacctggctt gctgagtagc agccacgtgc tccattgtcc atgcggaaaa taacggtgcc
     2641 catgtcttct gtatcgacgg ggtaggtagt gtaggcgtcg ggaggaagcg ccttgccggc
     2701 aaaggcttcg atctggccct tgggcttccg gcgagttttg tagaaggtgt acaaatcggc
     2761 gcagacttcg gtaatgcgtt ttccggtgac gtgctcggtc atgtcacaga aatgcgagcc
     2821 gacgtccgcc atgcaccgtg acgcgccatt ctccttggac tcaattcgcc agttgtaatc
     2881 ggtatccaaa agcagccaat cctgcgaata cgtgccctga acgacgatga tttcgccgag
     2941 ctcgcccgcc tcgaccatgc ggcgcatgtg ctgcacgaga gggtagtaac gcaggttgtg
     3001 ggccgtgcaa tttctgagtt ttttctgggc cgctatctgg acgag
//
LOCUS       DATNDT010000354         3216 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1794536, whole genome shotgun sequence.
ACCESSION   DATNDT010000354 DATNDT010000000
VERSION     DATNDT010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3216
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1794536"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VN788_11365"
     CDS             complement(<1..205)
                     /locus_tag="VN788_11365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU21172.1"
                     /translation="MTPFALKDWFQREVLPLEAILVTYLRQNWRDQSNIEDLLHDVYL
                     QVYRGARERLPENPRAFVFTTARN"
     gene            complement(718..1821)
                     /locus_tag="VN788_11370"
     CDS             complement(718..1821)
                     /locus_tag="VN788_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HXU21173.1"
                     /translation="MAWNWQQADWPNFSYNPRALELLEQQFLRQSGEVIGAVRHVGLD
                     DQEALRIELISEEAVKTSQIEGEILNRDSVQSSLRYQFGLGPDQPNIPPAERGIAQMM
                     IDLYEHFDAELTNATMFAWHSMLLRGETSIKTIGGYRMHDDPMQVVSGPIHKRTVHFE
                     APPSDAIPAEMQRFIAWFNDTAPKGAHALPPLTRAGLVHLYFVCIHPFEDGNGRIGRA
                     LAEKSLAQNLGYPTLIALAYTIERKRKDYYSALEHNNKDLEVTDWLEYFARTVLEAQG
                     NTIKRVDFYVAKAKFYERLRDQLNERQAKVIARMFRESIDGFKGGLSAENYITIAQTS
                     RATATRDLQDLVEKGAVIRTGERRHTRYSLALE"
     gene            1999..2235
                     /locus_tag="VN788_11375"
     CDS             1999..2235
                     /locus_tag="VN788_11375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21174.1"
                     /translation="MGNKKDVWGSAALSIALTIIPMTASVYCVLSFALIMIAVRVIWT
                     NLRSPWTRGASTIVTVLLLASLLLPEIGRKIVSP"
     gene            2683..2883
                     /locus_tag="VN788_11380"
     CDS             2683..2883
                     /locus_tag="VN788_11380"
                     /inference="COORDINATES: protein motif:HMM:NF024139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU21175.1"
                     /translation="MTKLLSKKQVAQIVQMHPESIMRLSREGKFPPFLKVGTGTGAAV
                     RFVESDIEDWIKQRRGREFDAS"
     gene            complement(3078..3152)
                     /locus_tag="VN788_11385"
     tRNA            complement(3078..3152)
                     /locus_tag="VN788_11385"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3118..3120),aa:Met,seq:cat)
BASE COUNT          715 a          881 c          886 g          734 t
ORIGIN      
        1 gattgcgcgc ggtggtgaag acaaaggcgc gcggattttc cggcaggcgc tcgcgcgcgc
       61 cgcgatagac ctgcagatac acatcatgga gcaggtcctc gatgttgctc tgatcgcgcc
      121 agttctggcg gagataggtc accaaaatcg cctccagcgg cagcacttcg cgttggaacc
      181 agtctttgag ggcgaacgga gtcatgcctg cgcctgcgcg cggccaatcg gcgccgtcac
      241 ggctggtttt cgatgcgtcc gacatccccc gccacgtccc gctgttcgag ggttccaatg
      301 tactgcgctt acaaggacgc ttggcccctc aaaagccgaa caggcatgcg caaaaaaatt
      361 tataccggat gagcaatatt attgcgcgat gctcggagcg gttattgcgc aaaagccctg
      421 aggttaggcc gaggtcttgc cgccaggaca acgaaagagt tcggcttccg catcgtatgt
      481 gaaatcgcta tgcaggaaga tatgtctgtc cgagtcgact tctagtagcc gacatttttt
      541 tgtggtcaaa agagaggagc ggcgaggtct ccctcgccgc tttctctcgc agacccgaaa
      601 ttacgaaggt gtcgcccatg ccttaaaggt aggcattcag gcactttttc gcttttccct
      661 ttgcgccaac atatcgaact tttgcgtgcc gcttgcgtgc cgcgtgaatt cgcgtcgtca
      721 ctcgagcgct aaggagtagc gtgtgtgtcg ccgttcgccc gtgcggatga ccgcaccctt
      781 ctcaacaagg tcttgcaaat cgcgcgttgc cgtcgcgcgc gatgtctggg cgatagtgat
      841 gtagttctcg gctgagagac ctcccttaaa gccgtcaatg ctttccctga acatgcgagc
      901 tataacctta gcttggcgtt cgttgagctg atcacgcagg cgctcgtaga actttgcctt
      961 tgcgacgtag aagtcgacgc gcttgatcgt attgccctgg gcttcgagga cggtacgagc
     1021 gaagtattcc agccagtcgg tgacttcgag atctttgttg ttgtgttcca gcgccgaata
     1081 atagtctttg cgcttgcgct cgatggtata ggcaagcgca ataagggtcg ggtagccgag
     1141 gttttgggcg agcgacttct cggcaagcgc gcgcccgata cggccgttgc cgtcttcaaa
     1201 cgggtgtatg cagacaaagt agagatgcac aagacctgcg cgggtcaaag gcggcagagc
     1261 atgagccccc ttgggtgcag tatcgttgaa ccacgcgatg aaacgctgca tttccgcggg
     1321 tatggcgtcc gacggcggcg cttcaaaatg aactgtccgt ttgtgaatgg gtccggagac
     1381 gacctgcatc gggtcgtcgt gcatgcgata accacctatg gtcttgattg acgtttcgcc
     1441 tcgcaacagc atgctatgcc acgcaaacat cgttgcattg gtaagctcgg catcgaaatg
     1501 ctcgtagaga tcgatcatca tctgcgcgat gccgcgctcg gccggcggga tgttgggctg
     1561 atcggggccc aggccgaatt ggtagcgcag agatgattgg acactgtcgc ggttgagaat
     1621 ctcaccctcg atttgagagg tcttgaccgc ctcttcgctg atcagctcga tcctgagggc
     1681 ctcctgatcg tccagaccga catggcggac agccccgata acttctccgg actgacggag
     1741 gaattgttgt tccaaaagct ctaaagccct tgggttataa gagaaatttg gccaatcggc
     1801 ctgttgccag ttccaagcca tgagttatag aatccaattc tatagctcat aatatgcatt
     1861 ttttgtgagt tataggatag cccctctata gctcatatgt gacgcaagtg atcacatcag
     1921 cttacgattc cgaataaatt ccttgacgca aggaccatta tcgtacactg tttgttctca
     1981 agaagagaaa agagacaaat gggtaataag aaggatgtct ggggcagcgc cgcgctcagc
     2041 atcgcactga cgatcattcc aatgacggcg tcggtctact gtgttttgtc ctttgcctta
     2101 atcatgatcg ctgtccgcgt catctggaca aacctccgat caccttggac ccgcggggcg
     2161 agcaccatcg tcaccgtctt gcttctagca agtctgctac tgccggagat cggccggaaa
     2221 atcgttagcc cgtaatgtgc tgcacgttaa atacgcgaac agctgtttca cgacctcgca
     2281 cttggccatc gggacacggc actacacgaa tatttttctc cgccaatgcg tcgcccaacg
     2341 cattgactat ggggcggacc tcggcctcgc tattcaccaa aactgccagc ggctaaccaa
     2401 ctgaacccat ttccgccacg atgagcccga ctaccttccg cgccgagcca aattgcctcc
     2461 ttatgcgaac caccgggacg ttccgcattc gggccgacgt gttgttgaat caagacgcat
     2521 tcaagtgttg ctattgagtc ggaacaaatt gtccgccatg cgcgcgctcg gaatgcatat
     2581 gcacctaata accttcgaag ctgagcggac aaaatctgtg atcggtcgca gctccttcaa
     2641 cgccggggaa ttcttctcgc tggctgcaaa tcccagatca gaatgacgaa gctgctatca
     2701 aaaaaacaag tcgcgcagat cgttcagatg caccccgaaa gcatcatgag gttatcgcgc
     2761 gagggcaagt ttccaccatt tcttaaagtc ggcacgggta ccggagcagc ggtacggttt
     2821 gtggaaagcg acatagagga ttggattaag caacggcgcg gtcgcgagtt cgatgcttcc
     2881 tagagccaat ccgactcacc caccgtgcgg agccacaacc ggtggaacct agcccgctaa
     2941 ggcgacgtct ggccgtagcc ccccaagttg aacggaaaat attttttcgc ccaagattca
     3001 tgtgccacca gcgtgaccca agaccgattt tgcagacgcg gatgcgagat cttttttggc
     3061 taagccattg attcagctgg tagcagcgga cggatttgaa ccgccgacca agggattatg
     3121 attcccctgc tctaccactg agctacgctg ccaaaaacca gccgaggcgc ggggtttaag
     3181 ggcggggtat gggcctgtca aggcgcgcaa atcagg
//
LOCUS       DATNDT010000355         5335 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4225126, whole genome shotgun sequence.
ACCESSION   DATNDT010000355 DATNDT010000000
VERSION     DATNDT010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5335
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4225126"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VN788_11390"
     CDS             <1..383
                     /locus_tag="VN788_11390"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HXU21176.1"
                     /translation="WEGFVSRESNRIIFSGAEFQADFKPGGSLAWVGPGPDGKPMTYV
                     RGRVLRFEPPKVFQYTFAMGQNDKASRATIELVPETEATKVTVTHDEWAEDDSTYAAC
                     TDGWPRILSRLKTLLETGKTFKPH"
     gene            complement(459..3395)
                     /locus_tag="VN788_11395"
     CDS             complement(459..3395)
                     /locus_tag="VN788_11395"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXU21177.1"
                     /translation="MAVRKTCASIQNNLRQYRLLRNVAVFVLLALTSGYLCAQEYNFR
                     NLGVTEGLTNLAIRQIYQDRVGFIWVSTENGIFRYDGDRFEAFGPEQGVPSTSVAAFG
                     DAPDGSLLVGGDFGLYQLTGNRFRRLPGVFRSVSWAQGIQSDGKGHTFIGTESGLVEL
                     YSESGNDGFATRKFPQVPETSGQGAYGILVDGDTLWYGCGEELCRMDRNKTTVLGRDN
                     GLPERAWLVIQKDRDGNLWVRAKNAGVYVLPAGQTRFRRPAAPIPGSEMGLVATDADG
                     RILIPSPDGLLIRDKKGWQKIDRSVGVRGAVYAAFEDRQHSLWIGLVGRGLVEWRGYR
                     EWENYSSASGLGSDIVYEILPRADGSLWVATEGGLFRGTRRQFGIAWKNVAGLDGFPV
                     HSLQMALGGDLWVGTETRGVAKVHARTGSVQWFGKEQGLLGSAAYILRFDREQRLWAG
                     TEMGLFVAKAPYQKFSRITELPSTRIWAIAEGTDGTVWAGGAGGLFGLLPGGWKNWTH
                     ADGLSNQEVLSLGAGTDGVMWIGYRHGGGIDRIHPRLGTISIEKGVQRSGSDGLVYFL
                     DFDASDRLWVGTEKGVDTWDGSRWSHYDTRDGLAWDDCNLNAFAEEADGTVWIGTSGG
                     LSRFKPRPHRAPESPPEVVFTKIVMGRTDVSGQHNPSSDIHSNSLVARYSAPNASREN
                     GVVFRYRLGGASLTWTETAQRELQFAALAPGAYRLQIEARDSDGVWSGHTAEFPFKIL
                     TPWYWTWWFVGICGLIPLSVGVGVFRLRLLGARRREHELQTLVETKTADLRRVNEDLL
                     RLSSVDSLTGLANRRVFDEVLEKEFVRMTRTSSEVSLLILDIDHFKALNDSEGHQRGD
                     QYLAAVGSELTRRAKRKIDLAARYGGEEFAVILSETNAAGAELVAESVRLAIASLELP
                     HPASPVAPFLTVSIGVATATLEWCSSPGELVAAADQALYDAKRSGRNRVNVARQGAVP
                     RKIVNA"
     gene            complement(3962..4339)
                     /locus_tag="VN788_11400"
     CDS             complement(3962..4339)
                     /locus_tag="VN788_11400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21178.1"
                     /translation="MAIKITHDANAATASVELTITQPLDDFDLTEVAAPIPRDIDPIL
                     ASQGFKDLLDEARAILEREFVDSNLSIAQLTGAICKDGAVYHPGIWLVVREANAVGKE
                     MSAAARTRIAAVAESVRSTLELS"
     gene            complement(4388..4819)
                     /locus_tag="VN788_11405"
     CDS             complement(4388..4819)
                     /locus_tag="VN788_11405"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HXU21179.1"
                     /translation="MKIRLIMLGRTRRAEVRALVDDYLGRIRHYAEIETTELREGSDS
                     AVRKLKLEPSATIVLLDAAGKQFTSADFARWLNDLRDRGTRELVFLCGDAAGFPESLR
                     RAAKQKLSLSTLTMPHEFARVILAEQIYRAFAILAGHPYPK"
     gene            complement(4828..>5335)
                     /locus_tag="VN788_11410"
     CDS             complement(4828..>5335)
                     /locus_tag="VN788_11410"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU21180.1"
                     /translation="LIALTNADLERAVRGGYFREDLFFRLNVVVIVVPPLRERRQDIR
                     PLAEHFLARLAPIHGRAGATMDSAAVDALEKYAWPGNVRELKNVIEHALIFAKDATLK
                     REDFPAVLHASTEASPAARPRSLEDLEKEAIIATLESTHYKIAQAADILGISRKTLLE
                     KRKKYGLA"
BASE COUNT         1147 a         1602 c         1461 g         1125 t
ORIGIN      
        1 tgtgggaagg ttttgtgtcg cgggaatcga accgcatcat cttctcgggc gctgagtttc
       61 aagcggactt caagcctggc ggatcgctcg catgggtggg cccgggaccc gatggcaagc
      121 ccatgaccta cgtccgaggg agggtcctgc ggtttgagcc gccaaaagtc tttcaataca
      181 ccttcgcaat gggccaaaac gataaagcct cacgcgcaac tatcgaactg gtgcccgaga
      241 ccgaagccac caaagtaaca gtgacgcatg acgagtgggc cgaggacgac tccacttacg
      301 ctgcctgcac cgatggatgg ccacgtattc tttcccgcct gaagactctg cttgagaccg
      361 ggaagacatt caagccccat tgatacaagg tgcgaatagt ctgaaatttt ccatccggca
      421 gtatgccccg agctaccccc tcagcacggc tcaagggttt atgcattcac tatcttgcgc
      481 ggaactgctc cctgtcgcgc cacgtttacc cgatttcgtc cactcctctt cgcgtcgtag
      541 agcgcttggt ctgcggcggc gaccaattct ccaggagaac tgcaccattc gagtgtggct
      601 gttgcgacgc ctatgctcac cgtcaggaac ggggcaacgg gtgatgcagg atgcggtaac
      661 tcgagactgg cgattgccaa ccgcaccgac tccgcaacta gctcggcacc ggcggcattt
      721 gtctccgaaa gaatcacagc gaattcctca ccgccatagc gcgcggccaa gtcaatcttg
      781 cgcttggccc tccgggtcag ttctgatccg accgccgcaa gatattgatc gccccgctgg
      841 tgtccctcgg aatcgttaag tgccttgaag tgatcgatgt cgagaatcag aagcgagact
      901 tctgaactcg tccgtgtcat acggacaaat tccttctcga gtacttcgtc aaacacccgc
      961 ctgttcgcca gcccggtcag cgaatcgacg gacgatagcc gtaacagatc ctcattcacc
     1021 cgccgcagat ccgcagtctt cgtttctacc aacgtctgga gctcgtgctc ccgtcttcgc
     1081 gctcccagca accgcaatcg aaagacaccg acacccaccg acaaagggat cagcccgcat
     1141 attccgacaa accaccacgt ccaataccac ggagtcagaa tcttgaatgg aaattcagcc
     1201 gtatgaccac tccacactcc atcactgtct cgcgcttcta tctgcagccg atatgctccc
     1261 ggcgccagtg ctgcaaactg gagttctctt tgagcggtct cagtccaggt taaactcgca
     1321 ccccccaagc ggtagcgaaa aacaactccg ttttcacggg aagcattcgg ggcagagtat
     1381 ctcgcgacga gagagtttga atgaatatcg gaggatgggt tgtgctgccc ggaaacatcc
     1441 gtccgtccca tgacaatctt cgtgaaaaca acctcgggag gtgattcggg cgcgcggtgc
     1501 ggccgcggct tgaaacgaga gagtccaccg cttgttccga tccagactgt gccatctgcc
     1561 tcctcggcga acgcgttgag gttgcagtcg tcccacgcca aaccgtccct ggtatcgtaa
     1621 tggctccagc gagaaccatc ccacgtgtct acgcccttct ctgtacccac ccacagccga
     1681 tctgatgcat cgaagtccag gaaatacacg agtccgtcgc tgccacttct ctggacgcct
     1741 ttctcgatgg agatggtgcc aagccgcgga tggatacggt cgataccgcc tccgtggcga
     1801 taaccaatcc acatgacgcc atccgtaccg gcgcctaacg aaagaacttc ttgattgctc
     1861 aagccatcgg catgtgtcca attcttccaa cccccgggca agagaccgaa gagcccgccg
     1921 gcaccgccag cccagaccgt gccatctgtt ccctcagcga ttgcccaaat gcgcgtggac
     1981 ggcaattcgg tgattcgcga aaacttctgg taaggagcct ttgccacaaa taggcccatt
     2041 tccgtaccgg cccagagcct ttgttcgcga tcgaagcgca agatgtaggc tgcgcttccc
     2101 aagagacctt gctctttacc aaaccactgc acgctgccgg ttcgtgcatg aactttggcg
     2161 acgccacgcg tttcagtacc aacccataga tccccgccca gagccatctg aagactgtgc
     2221 acggggaagc catcgagccc cgcaacattt ttccacgcga tgccgaattg tcgacgcgtc
     2281 ccgcgaaaca gtcctccttc ggtagctacc cagagcgacc cgtcggcccg cggtaggatt
     2341 tcataaacga tatcgcttcc cagcccgctt gctgaggagt aattctccca ttcccgatat
     2401 cctcgccact cgacgagccc tcggcccacc aagccaatcc atagagagtg ctgacggtcc
     2461 tcaaaggctg catagaccgc tccgcgaacg ccgacggatc ggtcgatctt ctgccagcct
     2521 ttcttgtctc ggatcagcaa cccgtctggt gacggaatta gaatccgccc gtcggcatcg
     2581 gttgcgacaa gtcccatctc gctgcctgga atgggcgctg ctggccttcg aaatctggtt
     2641 tggccggccg gcaacacgta cacacccgca ttcttcgcgc gcacccaaag attaccgtca
     2701 cggtctttct gaatcactag ccaggcacgc tccgggagtc cgttatctcg cccgagaact
     2761 gttgtcttat tccggtccat gcggcagagt tcctctccgc atccgtacca caaggtgtcg
     2821 ccgtccacca aaatgccata ggcaccctgc ccggaagttt cgggcacttg gggaaacttc
     2881 cgcgtcgcga acccatcatt cccggactcc gaatagagct ccacaagacc ggactccgtg
     2941 ccaataaaag tgtgtccttt accatccgac tgaatgcctt gcgcccaact tacggaccga
     3001 aaaacgccgg gaagtcttcg gaaacggttg cccgtcagtt ggtaaagtcc gaagtcgcct
     3061 ccaaccaaga gagatccatc gggagcgtcg ccaaacgcgg cgaccgaggt tgacggaacc
     3121 ccctgttccg gtccaaaagc ctcgaagcga tccccgtcgt accggaagat gccgttttcc
     3181 gtgctcaccc aaatgaaacc aacacggtcc tggtagattt ggcggatcgc taggttagtg
     3241 aggccctctg tgacccctaa gttgcggaaa ttgtattcct gagcgcacag ataccccgac
     3301 gtcagcgcga ggaggacgaa cacggcgacg tttcgcagca gccggtattg acgcaagtta
     3361 ttctgaatag atgcacacgt tttgcgtact gccaattctc attatcatgg tcggtttatc
     3421 gtcgagaatg ccatatcaga tcagggatca tcgccttcag gtgatggccg cgatccgcat
     3481 catcagagtg cgccgggctg cgccgcctga gacagagccc agtactgcat gctcaacccc
     3541 attttttccg caatcgaagg agtggcgatg tgagccggga cgaatcgagg actttgcggc
     3601 tggacgcgca atgacaataa gtcccataag aatgctcggt cgttatagtg tctagccgat
     3661 tcgcgctcgg cgacgacaca aaggccaact ctgtgtcgag ttgagtaccc gtccagtcgg
     3721 tttctttagt ttggttccgg ctgtcggcaa ctcgggaatt accgcagatt gaggtacccg
     3781 tcgaaggggc aaagtcctga ttatcgcggt gatcggataa ttgccacggg cgccgccgaa
     3841 atccgctcgc gaactaggaa gctccattcg gaactgccgc tgatttgcgg gcagatgacg
     3901 ggttactcgg aaactggccc gccactgcaa tacaggcccc gagggcagtt gaggcgtgat
     3961 cttaggaaag ctcaagcgta gaccgcacgg actctgccac agcggcaatg cgcgtgcgcg
     4021 ccgcggcaga catttccttg cccaccgcgt tcgcttcacg cacgacgagc cagattccgg
     4081 gatggtagac ggcaccgtcc ttgcagatgg cgcctgtgag ttgcgcgatt gaaagattgc
     4141 tgtccacgaa ttcgcgttcg aggatcgcgc gcgcttcgtc gagtaggtct ttgaatcctt
     4201 gcgaggcgag aatcggatca atgtcccgcg gaatcggcgc cgcaacttcg gtcaggtcga
     4261 aatcatcgag gggctgcgta atcgtcagct ccactgacgc tgtggcagca ttcgcatcgt
     4321 gcgtaatttt gatcgccatg aacggatatt ctcgcagaat gcgcgcgcgg aatctgccta
     4381 tttcgaatca tttcggatag gggtgaccgg cgaggatggc gaacgcgcga taaatttgtt
     4441 cggcaaggat gacgcgggcg aattcgtgcg gcatggtcag tgtggaaagc gaaagcttct
     4501 gtttcgcggc gcgccgcagg gattccggga atccggctgc gtcaccgcaa aggaagacga
     4561 gctcgcgcgt gccgcgatca cgcagatcgt tgagccagcg cgcaaagtcc gccgaagtga
     4621 attgtttgcc ggcggcgtcg agcaagacga ttgttgccga cggttcgagt ttgagttttc
     4681 gcactgcgga atcgctgcct tcgcgtaatt ccgtagtttc gatttcagcg taatgacgta
     4741 tgcggcccag ataatcatcg accagcgcgc gcacttccgc gcggcgtgtg cggcccagca
     4801 ttatcagacg gattttcata ggtgcagtta cgccaagccg tatttttttc gtttttcgag
     4861 cagcgttttg cggctgatgc cgaggatatc ggcggcttgg gcgattttgt aatgtgtgga
     4921 ttcgagagtg gcgatgatgg cttctttttc gagatcttcg agggaacgcg ggcgcgcggc
     4981 aggcgaagct tcggtgctgg catgaagcac agcggggaaa tcctcgcgtt tcagtgtcgc
     5041 atccttcgcg aaaattagcg cgtgctcaat cacatttttc aattcgcgca cattgccagg
     5101 ccacgcatat ttttccaaag cgtccacggc cgccgaatcc atcgtggcgc cagcgcgtcc
     5161 gtggattggc gcgaggcgcg cgagaaaatg ttcggcgagc ggacgaatgt cctggcgccg
     5221 ttcgcgcaga ggcggaacga ctatgaccac gacattcagc ctgaaaaata aatcctcgcg
     5281 gaaatagccg ccgcgcacgg cgcgttccag atcggcattt gtgagtgcaa tcagg
//
LOCUS       DATNDT010000356         5120 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2703505, whole genome shotgun sequence.
ACCESSION   DATNDT010000356 DATNDT010000000
VERSION     DATNDT010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5120
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2703505"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="VN788_11415"
     CDS             complement(<1..1139)
                     /locus_tag="VN788_11415"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXU21181.1"
                     /translation="MNPLRLLKSSSLYLIGNIANRAVGFVMVPLYTRYLSTSDYGILE
                     LVELTISLVIMSVGYYAITGSMVRIYYDWKETRERNAVVSSAIITMAVFGIFVTGAAD
                     LAAPWLSIALFHSTVQTSFIRYCFLATYIGSLMEPALSYLRLMDRALFFVSFSLVQLT
                     ITLGLNIYFIAFAGQGIWSFVLSKLIFCTLGGGFLLFIILREIGVHWRWEPTKRMMVF
                     GAPLILSAVGFFIIHFSDRFFLTRYSTLSEIGIYSLAYKFGFVITYLVAQPFTLVWNV
                     SLYRYLEQEGWREDFARVARFFIFCLFLAGVTLVLFAHELVAIMAAPAFRGAVAVIPV
                     VIAAYVFREVGDFFRNILYIIKDRSLHVGVIAAVCAVLDLGLNFVL"
     gene            complement(1165..2487)
                     /locus_tag="VN788_11420"
     CDS             complement(1165..2487)
                     /locus_tag="VN788_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative O-glycosylation ligase, exosortase A
                     system-associated"
                     /protein_id="HXU21182.1"
                     /translation="MRQALLIAITLPLCVIAPFAPEMGLYAYTWFALMRPDVLAWGSG
                     LPYSFVLAAATLLGSLRYAGNFIRLFDTPISLALVCLQFPIAASVLFAQDTMATHDPF
                     KQYMRMMVMAFLIPLLMVSLEDLRRLLLVMGISLGLLGTRFGLYGLIHGGVRFNAGYG
                     GMLADNNCLALALVMGVPLCWYGRFLVKRLWLKQVYTWAAIISLPAIVMTYSRGAALS
                     LAAVLLVLAVRAKRKFAAGLILVMIAGGSVFLVGRSYLSRMQTIKAPTEEGSAAARLA
                     YAGVAFRMWKDHPIAGVGYGMTNEMKLMPYYITNLTEHGEQVIHDTYLQMLCDSGIFA
                     FLIYVGLLWGTIAWLGLSVHRMKRSHPELAPYPMMLQTSLLGFAIGSTFLSRIEFDLI
                     WMLLLASAVWYRIERQLRSKPTAGSSAADWVFEQPAPAVIEPAWEHRG"
     gene            complement(2528..3085)
                     /locus_tag="VN788_11425"
     CDS             complement(2528..3085)
                     /locus_tag="VN788_11425"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HXU21183.1"
                     /translation="MAATVTLTGSQFDALPYEDGRRWELLDGELIPVSSPTPERQLIL
                     QRILLALMIHLRANPGQGLALTDVEFALDENYRVRPDVLVLLHDRAHSLDMKKIPVPG
                     APDLAIEIISPSERSSDTQEKLQAYLRYGTREVWQVYPKSRSVVVYRGGESSTLADGE
                     LSTPLLEGFSWRFGRFSNRRLTFAR"
     gene            complement(3137..4366)
                     /locus_tag="VN788_11430"
     CDS             complement(3137..4366)
                     /locus_tag="VN788_11430"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020473120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HXU21184.1"
                     /translation="MQDDQSSRRRFLTGAAALAVGNPASVRAAKKNPAGGGILAYVGS
                     YSSPQGPEGSRGRGKGIYLFEVNRSTGGLTQREIVPNESNPSWLAFNPAKTHLYSGNE
                     TGTFEGAASGSVSAYRVDPASGHLMQLDTVSSRGAGPAQLSVHPSGKFVLVANYAGGT
                     VAVLAVRGDGSLGETTDVKHDSGAVGPAKATSAPPGSFAISGHDRPHAHMIHSDPSGR
                     FVLATDLGMDRIFVWKFDDDKGKLLETGHGSVALPPGDGPRHFCFHPNGRWMYSLQEE
                     GSTLVLFDFDPSSGALAARQTISTLPKGFAGTSFTSEVALSPDARFVYAANRLHDTIA
                     WFSVGSTGTLTFSGEEWTRGDYPRSFSIDPSGNFLYSCNQRSDVIATFRVHRETGRLA
                     FTGQYTPVGTPAILVFR"
     gene            complement(4473..4781)
                     /locus_tag="VN788_11435"
     CDS             complement(4473..4781)
                     /locus_tag="VN788_11435"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU21185.1"
                     /translation="MNGSQAVRDKDIDRAMSNIARDILSFDVINPLQYRGSDSLRKRA
                     AEWFASFEGLTGFELRDLSVVTGGDVAFSHSLNRVSATKKDGQNFEMWWRASVCYRKN
                     "
     gene            4911..>5120
                     /locus_tag="VN788_11440"
     CDS             4911..>5120
                     /locus_tag="VN788_11440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HXU21186.1"
                     /translation="MHGPLALLIASFVVGIMIGLTSMGGAALMTPFLILVVGVRPTFA
                     VGTDLVYAAVAKWLGAWVHWKQGTVD"
BASE COUNT         1272 a         1527 c         1319 g         1002 t
ORIGIN      
        1 agcacgaaat tcaggcccaa atccaacacc gcgcacaccg ccgcgatcac tccaacatgc
       61 aacgaacggt ccttgatgat gtacagaata ttgcggaaaa aatctcccac ttcccgaaac
      121 acataagcgg cgataaccac cggaatcacc gccaccgctc cgcgaaacgc cggggctgcc
      181 ataatcgcca ccagttcgtg cgcgaacaaa accaacgtca cgcctgccag gaacagacag
      241 aagatgaaga accgggccac gcgtgcgaaa tcctcgcgcc agccctcctg ttccaggtac
      301 cggtaaagac ttacgttcca gaccagcgtg aacggttgcg ccaccagata ggtaattacg
      361 aagccgaact tatacgccag cgagtaaatt ccgatctctg aaagcgtgct gtagcgtgta
      421 agaaagaacc ggtcggagaa atgaataata aagaagccga cagccgacaa aatcagcggc
      481 gcgccgaaca ccatcatgcg tttagtcggc tcccaacgcc agtgcacgcc gatttcccgc
      541 agaatgataa acagcaggaa tcctccgccc agagtgcaga agatcaactt actaagcacg
      601 aagctccaga taccctggcc ggcgaaggcg atgaaataga tattcaatcc gagcgtaatc
      661 gtcaattgga ccagactgaa agaaacaaaa aacaatgccc ggtccatgag ccggagatac
      721 gaaagcgccg gctccatcag gcttccgatg taagtcgcca gaaaacaata tctgataaac
      781 gaggtttgga cggtggaatg aaacagagcg atactgagcc agggcgcggc aagatcggcg
      841 gcgccggtaa caaagattcc aaataccgcc atggtgatga ttgccgaaga aaccaccgca
      901 ttgcgttcgc gggtttcctt ccagtcgtag taaatacgca ccatgctgcc ggtgatagcg
      961 taatatccga cgctcatgat caccagtgaa atagtcaatt cgactaattc aagaattccg
     1021 taatcggaag tcgagaggta ccgcgtgtaa agcggcacca tcacgaaacc aacggcgcgg
     1081 ttcgcgatat taccgatcag atagagcgaa gagctcttga gaagccgcag aggattcatg
     1141 cagtatgcgc gcgtccggag cccatcatcc gcgatgttcc caggcgggtt cgataacagc
     1201 tggagccggt tgctcgaaaa cccaatccgc ggcgctcgat cccgcggtgg gtttactccg
     1261 aagctgccgt tcgattcggt accagacggc gctcgccagc agcaacatcc agatcaggtc
     1321 gaactcaatg cgggaaagaa aagtgctgcc gatagcaaac ccaagcagcg acgtctgcaa
     1381 catcatggga taaggcgcca actccggatg agagcgtttc attcgatgaa cggaaaggcc
     1441 aagccacgcg atcgtacccc acagaagtcc aacatagatt aaaaacgcga aaatgccaga
     1501 atcgcacaac atttgaagat acgtatcgtg aatgacctgc tctccatgtt cggtaagatt
     1561 cgttatgtag tatggcatca gtttcatttc gttcgtcatg ccataaccga caccggctat
     1621 cggatgatct ttccacatac ggaaagccac tcccgcatag gccaaccgcg ctgccgccga
     1681 gccttcttcc gtcggggctt taatcgtttg catccggctc aaataactgc gccccactag
     1741 aaaaacactt cctcccgcaa tcatcaccag gatcaaaccg gccgcgaatt tgcgtttcgc
     1801 gcgaacggcg agcacaagca gcacggccgc caacgataac gccgccccac gcgaataggt
     1861 catcacaatg gccggaagcg agataatcgc cgcccacgta taaacttgct tcaaccacag
     1921 gcgctttacg agaaaccgtc cgtaccaaca aagtggcacg cccattacga gagccaatgc
     1981 caggcaattg ttatccgcca acatcccacc atatcccgca ttgaaacgaa ctcccccgtg
     2041 gatcaaccca tacaatccga acctggtgcc cagaagccca agggatattc ccatcaccag
     2101 cagaagccga cgcaaatcct ctaggctcac catcaacagc ggaatcaaaa acgccatcac
     2161 catcatccgc atatactgct tgaatggatc gtgagtcgcc attgtgtctt gcgcgaataa
     2221 caccgaagcc gcgattggaa actgcaggca aaccagcgcc agagaaatgg gcgtatcgaa
     2281 caggcgtata aaattccccg catatcgaag actgccgagc agagtcgcgg cggccagcac
     2341 gaacgaatac ggcaaccccg aaccccaagc tagaacgtcc gggcgcatta aagcgaacca
     2401 cgtataggca tacagcccca tttccggagc gaacggcgca atcacgcata gcggcagtgt
     2461 gattgcaatt aggagtgcct gcctcaaaga aaaaccttgt ctaattataa gttggctgga
     2521 gccggtttca tctcgcgaag gttagacgcc ggttagaaaa gcgcccgaat ctccaggaaa
     2581 atccttccaa aaggggagtc gaaagctcgc catccgccag ggtcgagctt tccccgcccc
     2641 gatagaccac gaccgagcgg gatttcggat aaacctgcca gacttcccgc gtcccataac
     2701 gcaaatacgc ctgaagcttt tcctgcgtat cggagctgcg ctcgctagga gaaatgatct
     2761 cgatcgccaa atcgggggcg ccaggcacgg gaatcttctt catatccagg ctatgcgcgc
     2821 ggtcgtgaag caagaccagt acgtccggtc tcaccctgta attctcatcg agcgcaaact
     2881 ccacatccgt tagcgcaaga ccctgcccgg gattcgcccg gagatgaatc ataagcgcca
     2941 gcagaatccg ctgaagaata agttggcgct ctggagttgg actcgacacg ggaatcagct
     3001 ctccgtccag gagttcccag cgccgcccgt cttcataggg cagcgcgtcg aattgcgaac
     3061 cggtcaaagt aacagtcgcc gccatagaag ctcctgtgtc tattatcaaa aacgccgtca
     3121 agacgccaag cgacgttcat ctaaacacca gaatcgctgg cgtccccacc ggagtatatt
     3181 gcccggtgaa tgccagcctc cccgtttccc ggtgaacgcg gaacgtggcg atgacgtcgc
     3241 tcctttgatt gcaggaatag aggaaattgc cgcttggatc gatgctgaag ctgcgcggat
     3301 aatctccccg ggtccactct tcgcccgaaa acgtcaatgt ccctgtactt cccactgaaa
     3361 accaggcgat ggtgtcgtgc aggcggttgg ccgcatatac aaacctcgca tcgggcgaca
     3421 gcgccacttc tgacgtgaag cttgtgcccg cgaagccttt cggcagcgtc gagatcgtct
     3481 gccgtgcggc cagtgctccg ctcgatgggt cgaagtcgaa cagcacgagc gtcgagccct
     3541 cctcctgcaa cgagtacatc cagcggccgt tcggatgaaa gcagaagtga cgcggcccgt
     3601 ctcctggtgg gagtgccacc gagccatggc ccgtctcgag cagcttgcct ttatcgtcgt
     3661 caaatttcca gacgaagatc ctatccattc ccaggtccgt cgcgagcacg aatcgcccgg
     3721 aaggatccga atgaatcata tgggcgtggg ggcgatcgtg gccgctgatg gcaaaacttc
     3781 cgggcggcgc actggtggcc ttggccggac ccaccgcacc gctatcgtgc ttgacgtcgg
     3841 tggtctcgcc gagcgacccg tccccccgga ccgccagcac cgcgactgtt ccaccggcgt
     3901 agttggccac cagcacaaac ttgccggacg ggtgaacact caattgggcg ggaccggccc
     3961 cgcgcgagct gactgtatca agctgcatca aatgcccgct ggcgggatca acccggtacg
     4021 cgctcaccga gccagatgcg gcgccttcga atgttccggt ctcgttcccc gagtacaagt
     4081 gtgtcttcgc gggattgaaa gcgagccacg atggattcga ttcgttcgga acaatctcgc
     4141 gctgcgtcaa gccgcccgtc gaacggttca cttcgaacag gtaaatgccc ttccctcttc
     4201 cccggctgcc ttccggcccc tgtggcgaac tgtatgagcc gacatacgct aaaatcccgc
     4261 cacccgccgg attctttttt gcggctcgta cacttgcggg attccccacc gccagcgctg
     4321 ccgctcctgt cagaaacctg cgtcgcgaac tctggtcatc ctgcacgatg tgactcctcc
     4381 tcttaggcta cggcttgaga tcaagcgacg ctttgccctt ctgtgggtcg aaaggtaccg
     4441 aattatgctc atgaatgacc gtccataagt cgtcaatttt tgcggtagca aacgcttgcg
     4501 cgccaccaca tttcgaagtt ttgtccatct tttttcgtcg cgctgacccg gttgaggctg
     4561 tggctgaaag ccacatcgcc gcccgtaacg acacttagat cccgaagctc gaagccggtc
     4621 agaccctcaa acgaagcaaa ccattccgct gcgcgttttc tcaacgaatc tgatccgcga
     4681 tactgcagcg gatttatgac gtcgaatgat aagatgtctc gcgcaatgtt agacatagcc
     4741 cggtctatgt ccttatcgcg gactgcttgg gacccgttca tcgatcagcg ctcggatctg
     4801 ggcttcatcg ctttgccact ttccatcgtt ccattatcac cggggctatt caatataggc
     4861 caaatatcat atatactatc ggaatgatcg acattgtagg gatgtagact ttgcacggac
     4921 ctcttgcact cttgatcgcc agttttgtgg tcggtatcat gatcggcctc accagcatgg
     4981 gcggagccgc gctaatgacg cctttcttga ttctggttgt tggggtgcgt cctacattcg
     5041 ccgttggaac ggatctggtg tatgcggctg tagcaaagtg gctgggggcg tgggtgcact
     5101 ggaaacaggg cacggtggat
//
LOCUS       DATNDT010000357         4331 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3763774, whole genome shotgun sequence.
ACCESSION   DATNDT010000357 DATNDT010000000
VERSION     DATNDT010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3763774"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..661)
                     /locus_tag="VN788_11445"
     CDS             complement(35..661)
                     /locus_tag="VN788_11445"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HXU21187.1"
                     /translation="MPVYVVEEGEHISGIAEKFGFRDFRTVWNDPHNAKLRAKRKNPH
                     VLLPGDELFIPEKEIRTENRPTAKLHAFQIPSGKLKLKVLIQNVDGDPIDDTTVSLLA
                     GSEASRVKTQQGIVEKEIPRNAHDGKIELEDDQIPMGIGDLDPVDSESGQLQRLMNLG
                     YYRKPISPIDQDEWRSGVEEFQCDHDLNPPTGVVDGQTQDKLEEAHGC"
     gene            complement(683..1324)
                     /locus_tag="VN788_11450"
     CDS             complement(683..1324)
                     /locus_tag="VN788_11450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21188.1"
                     /translation="MSTPHHRKEWRLRDIRGEWIVLRWFDLAEGEELWKGPFSVSVLL
                     NQWFSDSACRPVLRDLCWELWGGPAYRPDHLRRNRDLQALLIEEFHCGRLLLIGPKTP
                     PPRGITGIRAGSGRDSNLAAILAASRAKQAAIKQRDDAARRASKQKTWVAIRLQDEKG
                     QPIPDERYRVVLPDGSVQEGKLNAKGEAYFYEINPGQCQISFPDVHRSEWKPA"
     gene            complement(1510..1986)
                     /gene="bfr"
                     /locus_tag="VN788_11455"
     CDS             complement(1510..1986)
                     /gene="bfr"
                     /locus_tag="VN788_11455"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020734067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HXU21189.1"
                     /translation="MKGNPKVISALNEALKEELTAINQYFLHAEMCENWQYARLAAHI
                     KKESIDEMKHAEALMERILFLDGTPNLTEPLQLSVGSDVKAQLESDLKLEIEAVTMYN
                     RFVRLARDEGDNTSRELFERLLKDEEAHVDWLEAQVHQVRELGYERYLSQQLRQPE"
     gene            2128..2676
                     /locus_tag="VN788_11460"
     CDS             2128..2676
                     /locus_tag="VN788_11460"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU21190.1"
                     /translation="MKTFLALPLLAAVAAFGAAPSTPSVSQMYNQQLTMLEHELVPLV
                     EAMPADKFNFAPTNGNFKGVRTFGQQASHVAEVIYSVSASILGEKSPTEEGKNENGPA
                     SLKTKDDVVKYLKGAFAYSHKAMESLTQSNIFDMVQSPFGKQKVPRLSIVDIPIWHSF
                     DHYGQMVVYLRMNGIVPPASRH"
     gene            complement(2681..3280)
                     /locus_tag="VN788_11465"
     CDS             complement(2681..3280)
                     /locus_tag="VN788_11465"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HXU21191.1"
                     /translation="MARELWLARHGETEWSKSGAHTGRTDLPLTEEGCKRAAAMRTYL
                     NGRPFALVLTSPKTRARETCRLAGYGDVAQIEPNLQEWDYGDYEGRTTADIQKERPGW
                     SLWTDGVPNGETIEQVAARADAVIARALEADGDVALFAHGHILRILTARWVGLPPQGG
                     RLFALGTASLSRLGYERDTRVITKWNQTFPLDLASHRQA"
     gene            3375..>4331
                     /gene="gnd"
                     /locus_tag="VN788_11470"
     CDS             3375..>4331
                     /gene="gnd"
                     /locus_tag="VN788_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HXU21192.1"
                     /translation="MQLGMIGLGRMGANMVRRLLRGGHECVVFDTNTANVQQLSDEGA
                     TGASSLEDFFAKLAKPRAIWMMVPAAVVDSMLEKLAPKMEQGDTIIDGGNSYYIDDIR
                     RAKDLESKGIHYVDVGTSGGVWGLDRGYCQMIGGQTEVVKHLDPIFKALAPGRGNIDR
                     TPGREKTAGTAEDGYLHCGPSGAGHFVKMVHNGIEYGLMAAYAEGLNILKHAGAGKQG
                     RLMDAETAPLRDPEHYQYDFNLADVTEVWRRGSVIASWLLDLTAISLAEEPDLKKFSG
                     RVSDSGEGRWTLLAAIESSAPAPVLSAALYQRFASRGEDDFAE"
BASE COUNT          877 a         1287 c         1226 g          941 t
ORIGIN      
        1 ccgccgctcg gagcttgggt agggtccgcc ataatcagca gccgtgcgcc tcctcgagct
       61 tgtcttgcgt ttgcccgtcg acaacgcccg tcggcggatt aagatcgtga tcgcattgaa
      121 attcctcgac tccggaccgc cattcatcct ggtcgatggg agagatcggc ttgcgatagt
      181 accccaggtt catcaggcgc tgcaattgac cgctctcgga atcgaccggg tccagatcgc
      241 cgattcccat gggaatctgg tcgtcctcca actcgatctt gccgtcgtga gcgtttcggg
      301 gaatctcttt ttccacaatg ccctgttggg ttttgacccg ggacgcctcg ctgccggcca
      361 gcaggctcac cgtcgtatcg tcgatcggat cgccgtcgac attctggatc agcaccttca
      421 gcttcagctt gccggaggga atctgaaagg catgtaactt ggcggtcggg cgattttcgg
      481 ttctaatttc cttttccgga ataaacagtt cgtctcccgg aagcagcacg tggggattct
      541 tcctcttggc gcggagcttc gcgttgtggg gatcgttcca aacggttcga aaatcccgaa
      601 agccgaactt ttcggcgatt cccgaaatgt gttccccttc ctcgacgacg taaaccggca
      661 caatacccct taatttcggc tctcaggccg gcttccattc cgagcgatgc acatccggga
      721 aagagatctg acattggcca ggattgattt cataaaaata agcttctcct ttggcattga
      781 gctttccctc ttggacggag ccgtcgggca acacgacgcg atatcgttcg tcaggaatag
      841 gttgaccctt ttcgtcctgt aaacgaatgg ctacccatgt cttctgtttg gaggcgcgcc
      901 gcgcggcatc gtcgcgctgc ttaatcgcgg cctgctttgc tctactggcc gccaggatcg
      961 ccgctaaatt cgagtcgcgg ccggagccag cgcgaatccc ggtaatcccc ctcggcggcg
     1021 gcgttttcgg cccgatcaac aagagccggc cgcaatggaa ctcttcgatg agaagcgcct
     1081 gaagatcccg gtttcgtcgc aggtggtccg gccgatacgc cgggccgccc cacaattccc
     1141 agcataggtc ccgcaaaacg ggccggcagg cggaatcgga gaaccactgg tttaaaagaa
     1201 cagagaccga aaagggtccc ttccataatt cctcgccttc cgcgaggtcg aaccaccgca
     1261 gaacgatcca ctcgccgcgg atatcgcgta accgccactc tttgcgatgg tgaggagtcg
     1321 acaagacggg ccccggcgcg gtgtggccga acggtcacgc cgcgtatgaa gtcgtgctct
     1381 gtgtcggaag atcgttacac aaatctacaa aactggcgcc gggcgctagc gatcttgctc
     1441 ggttcttagt tatgggaagg ctggacctga tacccagaag gccgtcgatc actgacggat
     1501 taacgtgggc tattccggtt gccgaagttg ttggctgagg tagcgctcat aacccagctc
     1561 gcggacttga tgtacttgcg cttcgagcca gtcgacgtgc gcctcctcgt ccttaagcaa
     1621 gcgctcaaat agttcccggg aggtgttatc gccttcatcg cgcgccagac ggacaaaacg
     1681 gttgtacatt gtgacggcct caatctctag ctttaggtcg ctttccagtt gcgccttcac
     1741 atcgctgccg acacttaact gcagcggttc ggtcaggttt ggagtgccat ccagaaacag
     1801 gattcgctcc atcagcgcct ccgcgtgctt catttcatca atcgattctt tcttgatatg
     1861 tgccgccagt ctggcgtatt gccagttctc gcacatttca gcgtggagaa agtactgatt
     1921 gatcgccgtc agttcttctt tgagcgcttc gttcaaggcg gaaatgactt tgggatttcc
     1981 cttcatctcc cgtattttat acgactggac atcgggaaac agttgcaagc gaacggcgga
     2041 gcatacatgt ttgaccatcg tgagccggac gagcctcggg tcttcagtcg tataatgact
     2101 ccgatccacc aaaattcagg aggcccagtg aaaacatttc tcgcattacc gcttttggcc
     2161 gccgtcgccg ctttcggcgc ggcgccatcc acgccgtccg tcagccagat gtacaaccag
     2221 cagcttacga tgctggagca tgaactggtt ccactggtgg aggcgatgcc ggcggacaaa
     2281 ttcaatttcg cgccaacaaa cggcaatttt aagggcgtgc gcaccttcgg gcagcaggcc
     2341 tcgcatgtgg cggaagtcat ttactcggtt tccgcgtcga tccttggcga gaagagcccg
     2401 acggaagaag gcaagaacga gaacggcccg gcatcgctca aaaccaagga tgacgtcgtg
     2461 aaatatctca agggtgcgtt cgcctactcg cataaagcaa tggaaagtct gacccagtcg
     2521 aatattttcg acatggtgca gtctcctttc ggtaagcaaa aagtcccgcg cctgtcgatc
     2581 gtcgatattc cgatctggca cagcttcgac cattacggcc agatggtggt gtatctccgc
     2641 atgaacggaa ttgtcccgcc ggcgagccgg cactaaccgt ctacgcctgc cggtggctcg
     2701 ccagatcgag cgggaacgtc tggttccatt tggtgatcac gcgcgtgtcg cgctcataac
     2761 cgagcctgct taaagaggcg gtaccgagtg cgaacagcct tccgccttgc ggcggaaggc
     2821 ccacccagcg agccgtcaaa atgcgcaaaa tgtgcccatg cgcgaagagc gcgacgtccc
     2881 cgtccgcttc aagagcgcgc gcgatcaccg cgtccgcccg cgccgccact tgctcaatcg
     2941 tctcgccgtt cggcacgccg tcagtccaca atgaccaacc gggtcgttcc ttttggatgt
     3001 cggccgtggt ccggccttcg tagtcgccgt aatcccattc ctgcaggttc ggctcgattt
     3061 gcgcgacgtc tccataaccc gccagccggc acgtctcccg cgcacgcgtt ttgggactgg
     3121 tcaacacaag cgcgaatggg cggccgttca gatacgtgcg catcgcggct gcccgcttgc
     3181 atccttcctc cgtcagcggc agatccgtgc gccccgtgtg cgcgcccgat ttactccatt
     3241 ccgtttcgcc gtgccgggcc agccaaagct ctcgcgccat ctattttttc ctcctccaac
     3301 cgtcctcaaa ccaaatgata cgcggctcgc attaagctga agtagaccaa ccaccgaaaa
     3361 ggagcaacgg cagaatgcaa ctgggaatga tcggacttgg gaggatgggc gcgaacatgg
     3421 tacgccgtct tttgcgcggt ggccatgagt gcgtcgtgtt cgacacgaac accgcgaacg
     3481 ttcagcaact ctccgatgaa ggagctacag gcgcctcgtc gctcgaagat ttcttcgcga
     3541 aactcgcgaa accgcgtgcc atctggatga tggtgccggc ggcggttgtc gatagcatgt
     3601 tggaaaagct cgcgccgaag atggagcagg gcgacacaat catcgatggc ggcaactcgt
     3661 attacatcga cgatattcgc cgcgccaaag acctcgaatc aaaaggcatt cactatgtgg
     3721 acgtgggaac cagcggcgga gtgtggggcc tggatcgcgg ttactgtcag atgatcggcg
     3781 gccagacgga agtggtcaag catctcgatc cgattttcaa agcgctcgct ccaggccgcg
     3841 gcaatatcga ccgcacgccg gggcgtgaaa aaaccgccgg cacagcggaa gatggatacc
     3901 ttcactgcgg gccttcggga gctggccatt tcgtcaagat ggttcacaac ggaattgagt
     3961 acgggctgat ggcggcgtat gcggaagggc tgaacattct gaaacacgcg ggcgccggca
     4021 agcagggccg cctcatggac gcggaaacgg cgccgctgcg cgatcccgag cattaccaat
     4081 acgatttcaa tctcgcggat gtaacggagg tctggcggcg aggaagcgtg atcgcttcat
     4141 ggctcctcga tctcaccgcg atttctttgg ccgaggagcc cgatctgaag aaattctccg
     4201 gacgcgtttc cgattccggc gaagggcgat ggacccttct cgcggcgatt gaatcgtccg
     4261 cgcccgcccc agttttgagc gcggcgcttt atcagcgttt cgcatctcgc ggcgaggatg
     4321 atttcgccga a
//
LOCUS       DATNDT010000358         9645 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_81442, whole genome shotgun sequence.
ACCESSION   DATNDT010000358 DATNDT010000000
VERSION     DATNDT010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9645
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81442"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="VN788_11475"
     CDS             complement(<1..519)
                     /locus_tag="VN788_11475"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HXU21193.1"
                     /translation="MKKFMWVALAAALFVIPSHAQSVTPAADVSPGYSYLRVGGSNGV
                     NMNGGTGAVTFNANNWLGLAADFGFYHASPAGVGVSASTYQFGPHISYRAGGKVVPFG
                     EALFGGSHLSASFGGATASSNPFAYTMGGGADLQLGASDKWSLRPEFAYVGMRENGAT
                     ENCMRISVGIVFH"
     gene            1034..1420
                     /locus_tag="VN788_11480"
     CDS             1034..1420
                     /locus_tag="VN788_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXU21194.1"
                     /translation="MAHPKLGKLEIRVMEAFWTKGPCSVREAQETFPEHNRPAYTTVQ
                     TTVYRLERKKALRCVKRIGNANVFEAAISRDDAQRSFIDELRSLFKGRAKLIMAHLVE
                     SGELSLDDIKEAENMLRTKSRKELSK"
     gene            1417..3171
                     /locus_tag="VN788_11485"
     CDS             1417..3171
                     /locus_tag="VN788_11485"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXU21195.1"
                     /translation="MSAELSSLTPLANHLWQSTLFVGLIWLLTLAFKKNRAAVRYWLW
                     FAASVKFLLPFSFLVALGSRFSWRSGAPVAQPQWSFVVDNAIQPFAASTAPPQVVSPH
                     ASFTLTSILIVVWLCGIAVGIGFWLKCWMQMRRVRKTAAPLPLGLPIPVLSSPSQIEP
                     GVFGIFRPVLLLPEGIESRLTPTQLDAIVAHEMAHVRRHDNLTAAIHMLVEIVFWFFP
                     VVYWLRVRLIEERENACDEVVISAGSEAESYAEGIIEVCKSYVESPATCISGISGSDL
                     KWRIARILNRHFGENLTRSKKVALVLASGAALIAPLVIGALNAPLLDAQSTSGDKPKF
                     EVASVKPCEPGSGRGGPGGAPNRLQWSPGRLTVPCQTAWVLIGWAYVRYADGRATPGN
                     VGGGPAENTPIEGLPSWGRSQSFTISAEVPGDASKEMMMGPMMQSLLEKRFKLKLHTE
                     NRVVPVYDLTVAKGGLKLPQTNPDAICRNAWDPKTPARKPGDPYDCNAVMPDITISDF
                     ATSLPVFVGRPVIDKTGITGRFAIHMAFSRPDMTQSADAPPSIFTALKEQLGLELTPS
                     KGPQPTIVIDHIEEPTPN"
     gene            complement(3638..5470)
                     /locus_tag="VN788_11490"
     CDS             complement(3638..5470)
                     /locus_tag="VN788_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21196.1"
                     /translation="MQRPFLSNIVRLSCLLFFTATLLPAETWVKAVSPHFTVISNAGE
                     KNARNVAANFERIRATLAILHPSLRVDASAQTIIFVPRDVSTFDKLMPSLKKEDDHVA
                     GLFREGWEHNYVIIRLDFPDEADTEVYHEYIHSLLHLNFTRIPTWLDEGLSDFYGSTR
                     FDGDKVYIGAPSPRLALLRGQTPFPLKILLAVDQRSPYYHDADKAGMFYAESWALTHY
                     LEFGEGMGNGQRLSAYVALLQKKANSLQAFEQTFGNLNDVNTGFREYISKFAFPALEM
                     KNPPKIDQSSFQSAPMSAAETDAEMGGFFNYMGSSETADKWLTDALAADPKLALAHEN
                     MAFYAFRKGDDDTARKEFDQAVALDPNSYLAVYYQAMMKYGAKRDSDSDVALGTAMSR
                     VLQLNPAFAPALIVQSQVLVRQNQLQAAFNAATKAWDLQPDRAGYLTHLAAILDFAHN
                     FQQAVQTADAVVSRWSFTDGAEAIAVADHARQLGNIAEKPEETTAETDEMKYAADLLS
                     AQGMIASVACEPSKPIQIVLTTPSNEQLHFEMSGGFSVGFSDTLWYGEDHFTFCYHLE
                     GMRAVVRYEKSKDAAPKNIVRWLELRDELIPMVVPPQPQSASTN"
     gene            complement(5756..8407)
                     /locus_tag="VN788_11495"
     CDS             complement(5756..8407)
                     /locus_tag="VN788_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU21197.1"
                     /translation="MRSGFHRIIARFRALFSSSTLDRDLNQELDSHVAMLTDENIRKG
                     MSPDEARRDALLRMGARESIKEQHRELRGLPFLETFMQDLRYALRMLRRDAAFAAFAI
                     LIVGIGIGASCTIFSVVDTLLLRPLPFTDPASLVWLSNRPESGAHDLSGQTTQVGYLL
                     DLRGQNHSFSDLAAYFAFYGVGDANLIGDGQPERFTRVPVTQNFFPLLGVNPFLGRQF
                     TADESRDGSPCAVMMSYGLWQRRFNADRSIVGRSINISGSLCTVVAVLPASFDFGDIF
                     APGTHVDLFVPFPLSDRTNRWGNTLSIIGRLKPGATLAAASAESSSLAINMKRDHPER
                     NGFTPELSYLSQYISGRIRPALILLACAVGVVMLIVCANLSNLLLARGASRQKEIAIR
                     SALGATKPRLIRQMLTESILLSSAGAVLGLLLAFVATRALSHLSGMAIPLLESVRVDG
                     LSFAFTVGAAILTGALFGLVPALHVPGPKLHESLKDAHRGSTRGVGHAWIRSALVISE
                     IALACVLVVGAGLLIRSFARVLAVNMGFNPQHAVALRIDPNSTLYKTQEQRNAYFTDA
                     LQRALDIPGVQAAGLTDSLPLGHNRTWWAGVQGRVYTPKELPLAFVRIVSDGYFKAMG
                     IALIEGRDFTDSDTPATRHVVVINQTLAQRNWPGEDPLGKMIQTNGGSMVVGVVADVR
                     HLALEESSGSEMYLPIRQTNDYPAVDLVVRSSLPTADIGSRLRQALRPIAPDLSISTL
                     RTLQALVDTSVSPRRFVVLLLAGFAAFALILASLGIYAVISYSVSQRTQEIGIRMALG
                     ASAGRVQTDILRQTLRLAVIGIGVGVVTSLLLAGILRTLLFGVNSTDPATFLAMLIIL
                     IVIAAIAGYIPARRAARIDPVTAMRTE"
     gene            complement(8412..8756)
                     /locus_tag="VN788_11500"
     CDS             complement(8412..8756)
                     /locus_tag="VN788_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXU21198.1"
                     /translation="MKDSRSDVLQGTLDLMVLKTLDAMGPMHGYGLARRIEQIGDEAL
                     AINQGTIYLCLIRLLQNKWISAVWGASENNRKARFYSITKAGRKQLIAETENWERIAG
                     VIGKVLQLSPRR"
     gene            9036..9404
                     /locus_tag="VN788_11505"
     CDS             9036..9404
                     /locus_tag="VN788_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21199.1"
                     /translation="MSTIIMQYFKQFARKALLPVTVLAALCAASSFAQPARTIRVVLS
                     PRSTADRFEVTKNFGQSCRNVLITSNEHQSDFMVVAGGWSGDYRFMVIQKGGGVLYVT
                     ETMLLSNSVKDVCKFLNTRT"
     gene            9427..>9645
                     /locus_tag="VN788_11510"
     CDS             9427..>9645
                     /locus_tag="VN788_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21200.1"
                     /translation="MIKHRGDVCLRGKARLLVFGDEQQEVAVICAAPTALGFLSAFFP
                     TFRVGLSYVAPTALNSYQVMAPQHFLYFR"
BASE COUNT         2216 a         2574 c         2886 g         1969 t
ORIGIN      
        1 gtggaaaacg attccgaccg aaattctcat gcaattctcg gtcgcgccat tttcccgcat
       61 acctacgtac gcgaattcgg gacgcaagga ccacttgtcg ctggcgccga gttgaagatc
      121 ggcaccgccg cccatggtgt aggcaaacgg gttacttgat gcggtcgccc cgccaaacga
      181 tgctgataag tgcgaaccgc caaacagcgc ctcgccgaag ggcacgactt taccgcccgc
      241 gcgatacgag atgtgaggac cgaattgata cgtcgaagcc gagacgccaa ccccggctgg
      301 ggatgcgtgg tagaaaccaa agtcagccgc taaccccaac caattgttgg cgttaaaggt
      361 aaccgcgccg gttccgccgt tcatattcac tccattggaa ccgccgacgc gcaggtaaga
      421 atatcccggc gagacatctg cagccggcgt aactgactgt gcgtgagagg gaatcacaaa
      481 cagtgccgca gcaagagcca cccacatgaa cttcttcatg cgaaaacctc cgttaagtgt
      541 cttattttta gttcgatccg ggcgactcgg acggcttcca ggagttcccc acgcaaggcc
      601 cgagcagcta tttagctggt acatcgcccc acacatacaa ggctagggga gcggagcggt
      661 agcgtcaatt tcggcctcgg gaaggcgttc ttgggcggaa actgtacgcc cggacaggcc
      721 gttcggggcg agctgaacga tggtacaagc cgaattgatg gccaatttgg caccttaaaa
      781 tgggcttaaa tggccttaag aggccaaaaa tggcgcgaat ggcgccgtaa aaaggcttaa
      841 ttttcgttag tggtcgaaga cggccccggt tcgacaaagg taacagccgc cgatcgaaag
      901 gcggtcgctg cgagatgtag aaaacgcgct gtccccggtc tccccgttat ccttgtttcg
      961 ctgcctatgg tagggaaaac ctcttgcctt gtgactatcg taatagtaga acagacgatc
     1021 aaggtagaaa taaatggcgc atcccaagct tgggaaactc gaaattcgcg tcatggaagc
     1081 cttctggacg aaaggcccct gttctgtgcg cgaagcgcaa gagaccttcc ctgaacacaa
     1141 ccgcccagcc tatacgacgg tacagacgac ggtgtatcgg ctcgaacgaa agaaagcttt
     1201 gcgctgcgta aagcgaatcg gcaacgcaaa cgttttcgag gctgcaatct cgcgcgatga
     1261 tgcgcagcgc agctttatcg acgaactgcg ctccctgttc aagggacgcg cgaaactcat
     1321 catggctcat ctcgtggaat cgggcgaact gagcctggac gacataaaag aagccgaaaa
     1381 catgttgcga acgaaatcga ggaaagagct gtcaaaatga gcgccgaact ttcatctttg
     1441 accccgttgg cgaatcacct ctggcagtcc accctcttcg ttggcttgat ctggcttctc
     1501 acgctggcat tcaaaaagaa tcgagcggcg gtccgatatt ggctgtggtt cgccgcgtcg
     1561 gttaaattcc ttcttccgtt ttcttttctt gtggcgcttg gcagccgatt tagttggcga
     1621 agcggcgcgc ccgttgccca gccgcagtgg tccttcgtcg tcgataacgc catccagccc
     1681 ttcgccgcat ccaccgctcc gccccaagtc gtttcaccac acgcgtcgtt cacgctcacg
     1741 tcaatcctca tcgttgtatg gctttgcggt atcgcggtcg gcattggctt ctggttgaag
     1801 tgctggatgc aaatgcggcg cgtccgcaaa accgcggctc cgctcccact tggcttgcca
     1861 atccccgttc tatcctcgcc ctcgcaaatc gagccgggcg tgtttggcat ttttcgtcct
     1921 gtgctgctgt tgcctgaagg catcgaatca cgcctcactc ccacacaact cgacgccatc
     1981 gtagcgcacg aaatggctca cgtgcgccgc cacgataatc tcaccgccgc cattcacatg
     2041 cttgtcgaga tagtcttctg gtttttcccc gtggtctact ggctccgagt acgtttgatc
     2101 gaagaacgcg agaacgcttg cgacgaagtc gtaatcagcg caggcagcga agctgaatcg
     2161 tacgccgagg gaatcatcga ggtctgcaag tcctacgttg aatctcctgc gacgtgcatc
     2221 tcaggaattt ctggttcgga tttgaaatgg cgtatagcgc gaattctcaa tcgccacttt
     2281 ggggaaaatc tgacacgcag caagaaagtc gcattggtgc tagcgagcgg cgcggcactg
     2341 atcgctccac ttgtaatagg tgccctaaac gctccactcc ttgacgcgca atccacatcg
     2401 ggcgacaagc caaaatttga agtggcgtcg gttaaaccgt gtgaaccggg atcgggcaga
     2461 ggcgggcctg ggggcgctcc gaatcggctc caatggtcgc ccggcagact aacggtgccg
     2521 tgccaaaccg cgtgggtttt gatcggttgg gcctacgttc gctatgccga cggccgcgcc
     2581 actcccggga acgttggtgg aggcccagcc gaaaacactc ccatcgaagg ccttccctcg
     2641 tggggacggt cgcagagctt tacgattagt gcagaagtgc cgggagacgc gagcaaggaa
     2701 atgatgatgg gcccaatgat gcagtcgctt ctcgaaaaac gttttaagct aaagcttcac
     2761 acggaaaatc gcgtggttcc cgtctacgat ctgactgtcg caaagggagg acttaagctc
     2821 ccgcaaacga atccggacgc tatatgtagg aacgcgtggg acccgaagac acctgctcgt
     2881 aagccgggcg atccctatga ttgcaatgcg gtcatgccgg acattacgat ttccgatttc
     2941 gcaaccagtc tccctgtgtt cgtgggacgc ccagtaatcg ataagacagg aatcacagga
     3001 cgcttcgcaa ttcacatggc gttttccagg ccggacatga cacaaagcgc ggacgcgcca
     3061 ccgtcgatct tcacggcctt gaaagaacag cttggactgg agctaacccc tagcaaaggc
     3121 ccgcaaccta cgattgtgat cgatcatatt gaggaaccga caccgaactg actgccgaat
     3181 actttcgggt aaatttgact cccggtatcg atgaaggacc gggtcaagcg gcgctatggc
     3241 agcgatcata agcgcatcat tacgttgagt tggttttgag gcgatgacat gagagcaact
     3301 tcaaaggctg cccgcctcac gcaggaaccg tccgtccccg ctgtgagtcg cggggcaaag
     3361 gcggccgcta cgcacgtcaa aggcacggca aaatcttaag gcaaacccca atttaaacgc
     3421 gccgaccgga ggccagcgct acaacataaa ggcaaaggtt gcccgactac gaaacgcaac
     3481 agcaaagcag agacgccgac cgagaagcgc tgacaaaata cgcgggcacc cttcggtgct
     3541 cagggcagca ggacggtcac cgctacgaaa cctgcacagc caagagtggc tgtgccacaa
     3601 gcgactgctg tgcacttatg ccgatcactt ccgatagcta gtttgtggat gcggattgcg
     3661 gctgcggcgg taccaccatg ggaatgagtt cgtcgcgcag ctcgagccat ctgacgatgt
     3721 tcttgggcgc ggcgtctttg cttttttcat agcgcacaac ggcacgcatg ccttcgaggt
     3781 gatagcaaaa cgtgaagtgg tcctcgccat accagagcgt gtcgctaaag ccgacactaa
     3841 acccgccgct catttcaaaa tgaagctgct cgttcgacgg agttgtaagg acgatctgta
     3901 tcggctttga gggctcgcat gcgacggagg cgatcatgcc ctgggctgac aacaggtcgg
     3961 ccgcgtactt catttcatcc gtttcggcgg ttgtttcctc gggcttttcg gcaatattcc
     4021 cgagttggcg cgcgtgatcg gcgacggcga tggcttcggc tccatccgtg aagctccaac
     4081 gcgaaacgac ggcgtcggcc gtctggacgg cttgctggaa attgtgggcg aaatcgagaa
     4141 ttgcggctaa atgcgtgaga tagccagcgc ggtcaggttg gagatcccat gcctttgttg
     4201 cggcattgaa cgccgcctgt aattgatttt gccttacgag cacctgcgat tgaacaatca
     4261 gcgcgggcgc gaaagcggga tttagctgaa ggacccgcga catcgccgta ccgagcgcaa
     4321 catcggaatc gctatcgcgt ttggctccat atttcatcat ggcctggtaa tagacagcca
     4381 agtagctgtt gggatcaaga gcgacggcct gatcgaactc ctttcgcgcg gtatcgtcat
     4441 ctcccttgcg aaaggcgtag aacgccatgt tttcgtgagc gagcgccaat ttcggatccg
     4501 cggccaaggc gtcggtcagc catttgtccg cagtttccga agaacccatg tagttaaaga
     4561 agccgcccat ttccgcgtcc gtttccgcag cggacatggg agcgctttga aacgacgatt
     4621 ggtcgatttt tggaggattt ttcatctcca acgccggaaa cgcgaactta gagatgtact
     4681 cacgaaaacc ggtgtttacg tcattcaggt ttccgaatgt ttgctcaaat gcctgaaggc
     4741 tattcgcttt cttctgaagc aatgcgacgt aggcgctaag acgctggcca ttgcccattc
     4801 cctcaccaaa ctccaaatag tgggtgagcg cccacgattc cgcgtagaac atcccggctt
     4861 tatccgcatc gtggtagtaa ggcgatcgct ggtcgacggc aaggaggatc ttaagcggga
     4921 acggcgtctg cccccgcagc agagccaggc gtggactggg agcgcctatg taaaccttgt
     4981 caccgtcaaa ccgcgtactg ccgtagaaat cgctcagtcc ctcatcgagc catgtgggaa
     5041 tgcgcgtaaa atttaaatgc aaaaggctgt ggatgtactc gtggtagacc tccgtatccg
     5101 cttcatccgg gaaatccagt cgaatgatga cgtagttgtg ttcccagcct tcgcggaaaa
     5161 ggccagctac atggtcatct tcctttttta aggacggcat cagcttgtcg aaagtcgata
     5221 cgtctcttgg cacaaagatg atcgtctgag cactggcatc cacgcggagc gaagggtgga
     5281 gtatcgcgag cgtggcgcga atcctttcaa aattagcagc tacgttgcga gcgttttttt
     5341 ctccagcatt gctgataact gtgaaatgag gcgaaacggc ttttacccat gtttccgccg
     5401 gaagaagcgt cgccgtgaaa aagagcaggc aggatagccg aacgatattg ctgaggaatg
     5461 gcctttgcat aaacggaccc cgttcttatc ccgagggctg ggctgcggtg cgtggaattt
     5521 ataacacgga aatgagaaca gttacttgtt aaatataaaa actggtgcta gctagaaagt
     5581 agatgccgat tcaccgcaag attggttaca agcagcactg aaccgacaca gccaagagtg
     5641 gctgtgccac aacaacccat cacggcggga gtggctgcga gacacacgag ccgcggcaca
     5701 gatgagactg tggccacgcg aactgtggca tagatgggac tgtggcacag aaacgttact
     5761 cggtgcgcat ggcggtgacg gggtcgatgc gggcggcgcg gcgggcgggg atgtaacccg
     5821 cgattgcggc gatgacgatg aggatgatga gcatcgcgag gaatgtcgcc gggtcggtgg
     5881 agttgacgcc gaagagcaga gtgcgcagga tgccagccaa cagcaatgac gtgacgacgc
     5941 caacgccgat gccgattacg gctaggcgga gcgtctggcg aagaatatcg gtctgcactc
     6001 tgcccgcgga cgcgcccagg gccatgcgaa ttccgatttc ctgcgtacgc tggcttaccg
     6061 aatacgagat gacggcatag attccgagcg aggcgagaat caatgcgaac gcagcaaaac
     6121 cggcgagaag cagcacgaca aacctgcgcg gcgatacgga tgtatcgacc aatgcctgca
     6181 acgtgcgcag agtggagatc gagagatccg gcgcgatggg gcgcaacgcc tgacgcaggc
     6241 gagagccgat gtcagccgtg ggaagagagc tgcgcacgac gagatcgacg gcaggatagt
     6301 cgttcgtctg ccggattggc aagtacatct cggaacccga actttcctcg agcgccagat
     6361 ggcggacgtc ggctacgaca ccgactacca tgctgccgcc gttggtttga atcatttttc
     6421 caagaggatc ttcgccgggc caattccgct gggccagtgt ctggttaatg acgacaacgt
     6481 ggcgagtggc gggcgtgtcc gaatcggtga aatcacgccc ttcgatcagg gcaataccca
     6541 tcgctttgaa atatccgtcg ctcacgattc gcacgaacgc gagggggagc tctttcggcg
     6601 tatagacacg gccctgaacg cctgcccacc acgtcctgtt gtgaccgaga ggaagtgaat
     6661 cggtcaatcc cgccgcctgg acgccgggaa tatcgagcgc gcgctgaagg gcatccgtga
     6721 aataggcatt gcgctgctcc tgagtcttgt agagcgtgga attgggatcg atgcggagag
     6781 cgacggcatg ctgaggattg aagcccatgt tgacggcaag aacgcgcgca aaactgcgaa
     6841 tgagaaggcc tgcgccgaca acgaggacgc atgcgagcgc gatttctgaa atcaccagcg
     6901 cgctgcgaat ccaggcatgg ccgactccgc gcgtggagcc gcggtgcgca tctttgagcg
     6961 attcgtgaag cttggggcca gggacgtgca gagcgggcac gaggccgaaa agtgctccgg
     7021 taaggatcgc ggcgccaacc gtgaaggcga aggaaaggcc gtcaacgcga acgctttcga
     7081 gaagaggaat ggccatgccg ctcaggtgag aaagagcgcg cgtagcgacg aaggcgagga
     7141 gcaatcccag cacggcaccg gcgctggaga gaagaatact ctcggtgagc atctgacgga
     7201 taaggcgcgg ctttgtagcg ccgagcgcgg accggatcgc gatttctttt tgtcgggaag
     7261 cgccgcgcgc gaggagcagg ttggaaagat tggcgcaaac gatcagcatc accactccga
     7321 cggcacaggc gaggaggatg agagcggggc ggatacgtcc gctgatgtat tgggacaaat
     7381 acgaaagttc gggcgtgaag ccgttgcgct cggggtggtc acgcttcatg ttgatcgcga
     7441 gcgaggagct ttcagcggat gcggcagcga gagttgctcc gggcttgaga cgaccgatga
     7501 tcgagagcgt attgccccat ctatttgtcc ggtcgctgag gggaaaaggc acgaagagat
     7561 ccacgtgagt gccaggcgcg aagatatcgc caaagtcaaa ggaagcgggc agaacggcaa
     7621 cgaccgtgca cagcgagcca ctgatgttga tcgaacggcc aacgatgctg cgatccgcgt
     7681 tgaagcggcg ttgccatagg ccgtaactca tcatgacggc gcatgggctg ccgtctctgc
     7741 tttcgtccgc ggtgaattgc cgaccgagaa aaggattcac gccgagaagc gggaagaagt
     7801 tttgcgtgac tggaactcga gtgaaccgtt cgggctgacc atcgccgatc aaattcgcgt
     7861 cgccgacgcc gtaaaaggca aagtacgcgg caagatcgga gaaagaatga ttttgaccgc
     7921 gcagatcgag aaggtaaccg acttgggtag tctggccgga cagatcatga gcgccggatt
     7981 ccgggcgatt tgagagccac accaagcttg cgggatcggt aaagggtagc gggcggagaa
     8041 gcagcgtatc gacgacgcta aaaatcgtgc aacttgcgcc gattccgatg ccgacgatca
     8101 ggattgcgaa cgcggcgaat gcggcgtcgc gacgcaacat gcgaagcgca tagcgcaagt
     8161 cctgcatgaa cgtttcaagg aagggcaggc cgcgcaattc gcggtgctgc tcttttatgg
     8221 attcgcgtgc gcccattcgt aggagggcgt cgcggcgcgc ttcgtccggg gacatgccct
     8281 tgcggatgtt ttcgtcggtg agcatggcga cgtgggagtc gagctcctga ttgagatcgc
     8341 gatcgagcgt cgacgaggaa aacagcgcgc ggaaacgggc gatgatgcga tgaaagccgg
     8401 atcgcatgat gctacctccg aggagagagc tgcagaacct tgccgatgac gccggcgatg
     8461 cgttcccaat tttcggtttc ggcgattagt tgcttgcggc ccgctttcgt aattgagtag
     8521 aagcgagcct tgcgattgtt ttcggatgct ccccatacgg cagagatcca tttgttctgc
     8581 aaaaggcgaa tcaggcagag atagatcgtg ccctggttaa tggcgagggc ttcgtcaccg
     8641 atctgctcga tgcggcgggc taggccataa ccatgcatgg gacccatggc gtcgagagtc
     8701 ttcaaaacca tgagatcaag agttccctgc aagacgtctg agcgagagtc tttcacgcta
     8761 tctcctgttt gttgcttaga ggaaggtacg cttggtcctg tgggttgtca agagggcgtg
     8821 acgcacgcga ataggcaacc taaggaagac gcggcgtact atttggccac cggttatgac
     8881 gcggactgcg gcacgcaacg ttttggctta aaatgtgttc tagatatacg taagtgtgcg
     8941 cagacggctg tctcgcctgt gcacggcagg catgccttga ccatttctat ttatagatgt
     9001 gatttttttc actaagagaa gaggagaaaa tcgcaatgag cacgatcata atgcaatatt
     9061 tcaagcagtt tgcaaggaag gcgctgctgc cggtgacggt gctggcggcg ttgtgtgcgg
     9121 catcgtcgtt cgcgcagccg gcgcgcacga tccgagtggt tctttcgccg cggtcaacgg
     9181 cggaccggtt cgaggtgact aaaaacttcg gccaaagctg ccggaatgtg ctgatcacga
     9241 gcaacgagca tcagtccgac ttcatggtgg tggcgggtgg ctggtccggg gattacaggt
     9301 tcatggtgat ccaaaagggc ggcggcgtgc tctacgtgac cgagacgatg ctgctgagta
     9361 attcggtgaa agacgtctgc aaattcctga atactcggac gtaatagcat tcccaatcac
     9421 gtcgggatga tcaagcatag aggcgacgtg tgcctgcgag gcaaagcacg ccttctggtc
     9481 ttcggggatg aacagcagga agtcgctgta atctgtgccg cacctacggc gctcggattt
     9541 ctttccgcat ttttccccac cttccgggtg gggctaagtt atgtcgcgcc tacggcgctt
     9601 aattcatatc aggtaatggc gccgcagcat tttttgtatt ttcgg
//
LOCUS       DATNDT010000359         3539 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1016083, whole genome shotgun sequence.
ACCESSION   DATNDT010000359 DATNDT010000000
VERSION     DATNDT010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1016083"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            449..2116
                     /locus_tag="VN788_11515"
     CDS             449..2116
                     /locus_tag="VN788_11515"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HXU21201.1"
                     /translation="MTIEREKLEVDVLIVGAGPAGLSCALHLSKLIDRHATEKKSPAL
                     SSENVYVLEKAREIGAHQLSGAIMDPRALRELVPDFEKTAPLDTPVTGDAAYYFTEKN
                     SYKLPITPPPLANHGNYVISLNKLVKWLGGLVEKKGVNVFTQFAGRELLYEKNGIVGV
                     LTEDKGVDKNGKPKDNFTPGYELRAKVTVLAEGPRGSLTKGLVSRLKLDGLNPQVYGL
                     GIKELWDVQPGRIDTGYVAHTLGWPLTSDLYGGGWIYGLRDNRVSLGIVVGLDYHKSL
                     FDPHEAFQKFKTHPFVKKILEGGKLVRYGAKTVPYGGWYSMPRSYFDGGLIIGDSASL
                     LNSQRLKGIHTAIKSGMLAAETIYEALCAGDTSAAKLSAYETKLEQSWLKKELWFVRN
                     FHQGFHDGLWKGLVHAGLQFVSGGRGLIDPMRAQPGYTEYTKLHRDRTLVDQSTRFKG
                     DGQLTFDRPTDVYHSGTRHDENQPCHLIVLDPNICSDRCVREFGNPCQYFCPAAVYEM
                     VMQQGVPKLKINAANCVHCKTCDIADPYQIIEWVPPEGGGGPNYEGM"
     gene            complement(2134..3312)
                     /locus_tag="VN788_11520"
     CDS             complement(2134..3312)
                     /locus_tag="VN788_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21202.1"
                     /translation="MSLKFQFAPDRATCSAHLRAAMAILAFTFLWLTPIARAQDAPPG
                     AVGRVQGRDISVESGTAANNGTARSVPSIYVLNGSVVTVHSAKARMTLFAGGTVDICG
                     PAKFTVLLSGDAITLALNFGTVRVSLPSKTSVRVFTPTVIGTPLDINGGSRDVTVGLN
                     LNDSLCVLATSGAIQLQNQFSDEKLIVPQKGEFFLNAGKLLPVAGTPGSCQCFADEPG
                     PTAPSPGPIPETAISIPPPPINKSPANSRAAEQPAEMKPAAESNVEYSVLADANEKHP
                     IERPPDDEVNSAPAVSMPIYTVVLPPLVFLASSPAPPPDPGPPMALLIREAQVSPDWE
                     FSGHVDAPEFATELQHALGRDARVATKKPQRGNSRKRSGFWGTLRKLFGGGDMEAKAE
                     "
BASE COUNT          772 a         1021 c         1020 g          726 t
ORIGIN      
        1 ctcaaaaccg tgccgctcga tcaacttccc gccatttccg ccgatctcga cgttccttct
       61 gagcttcagc gctttggcct aaagccaggc ctctccagcg cttcagcata agacggggaa
      121 ccggcggcca acgcaaccgc aagcagtcca caggaaccat cccttgcaac ccctccttac
      181 ggtgctaccc tgagccggaa aggggaacct tgatgtttgg agaaggcggg ttgaaagcga
      241 ggggaaaagg aagacgctct cgggtagacg ttctctcgaa gggtcgctgg atcgctttca
      301 tcgctcgatc cgccgcgaga agcacgcgtt tctgctcgac acttctgccg agtagaattc
      361 gatgtaactc gctgaaaaca caaggtgcct gtcacggcta cccgtcacaa aatcgaagtg
      421 tcttatttcc cgctttgggg ttccgtctgt gaccattgag cgcgaaaaac ttgaggtgga
      481 cgtcctgatc gtgggcgccg gaccggccgg gctttcctgc gcgctgcacc tctcaaagct
      541 gatcgaccgc catgctacgg agaaaaaatc tccggcgctt tcctccgaaa atgtttatgt
      601 cctcgaaaag gcacgggaga ttggcgcgca tcagctctcc ggcgcgatca tggacccgcg
      661 cgcgctcagg gaacttgtcc ctgactttga gaaaaccgcg ccgctcgaca cccccgtaac
      721 gggcgacgct gcctactact tcacagaaaa aaattcctac aagcttccca tcactccgcc
      781 gccgctggcc aatcatggta actacgtgat ttcgctcaac aagctcgtga agtggctcgg
      841 cggtcttgtc gaaaaaaaag gtgtgaatgt tttcacgcaa ttcgccggca gggaactgct
      901 ttacgaaaag aatggaatcg tcggtgtact gaccgaggac aaaggcgtgg acaaaaacgg
      961 caagccgaag gacaacttca cgcccggcta cgagctgcgc gcgaaggtta ccgtgctggc
     1021 ggaaggtccg cgcggttcgc tcaccaaagg cctcgtttcc cggctcaaac tagacggcct
     1081 caatccacaa gtttacgggc ttggtatcaa ggaactgtgg gacgtgcagc cggggcgtat
     1141 cgataccggc tacgtggcgc atacgcttgg ctggccgctc acttccgatc tttacggcgg
     1201 cggatggatc tacggtttgc gcgacaatcg agtttcgctc ggaattgttg tcggactcga
     1261 ttatcacaag tcgctcttcg atcctcatga ggcatttcaa aaattcaaaa cgcatccatt
     1321 cgttaaaaag atactggaag gcggaaagct cgtccgctac ggcgcgaaga cggttcccta
     1381 cggcggctgg tattcgatgc cgcgcagcta tttcgacggc ggcctgatca tcggagactc
     1441 ggccagccta ctgaattcgc agcgcctgaa gggaattcat accgcgatta aaagtggaat
     1501 gctcgccgcc gaaacgattt atgaggcgct ttgcgccggg gatacttcgg cggcgaaact
     1561 ctccgcatac gagacaaaac tggaacagag ttggctgaag aaagagctat ggttcgtccg
     1621 caattttcat caaggatttc acgatggcct atggaaaggg ctggtccacg ccggcttgca
     1681 attcgtcagc ggcgggcgcg gtttaatcga cccgatgcgc gcccaaccgg gctacaccga
     1741 atacacaaaa ctccatcgcg atcgcaccct cgtggatcaa tcgacgcgat tcaaaggcga
     1801 tggacagctt acgtttgacc gccccaccga tgtctatcat tctggaacgc gtcacgacga
     1861 gaatcagccc tgccacttga tcgttctcga tccgaacatt tgcagcgacc gctgcgtgcg
     1921 cgaattcggc aatccgtgcc agtatttttg tcccgcggcg gtttacgaaa tggtgatgca
     1981 gcagggcgtt ccgaaattga agatcaacgc tgcgaactgc gtccactgca aaacttgcga
     2041 cattgccgac ccctaccaaa ttatcgaatg ggttccacct gaaggtggcg gcggaccgaa
     2101 ctatgaaggg atgtaaatgt taggaggcgc ctgctactcg gctttggcct ccatatcgcc
     2161 tccgccgaag agctttcgaa gggtacccca aaagccgctc cgtttccggg agttcccgcg
     2221 ctgcggtttc ttcgtcgcta cgcgggcgtc ccgtcccagc gcgtgctgca attcggttgc
     2281 aaattccggc gcgtccacat ggccggagaa ttcccaatcc ggcgacactt gcgcctcgcg
     2341 gatcagcagt gccatcggcg gccccgggtc gggcggagga gcgggcgaac tcgccaaaaa
     2401 aaccagtggc ggcaggacaa cggtatatat tggcattgaa acggccggag ctgagttgac
     2461 ttcgtcatcc ggcggccgtt cgatcggatg cttttcattg gcgtcggcca gcacactgta
     2521 ttccacattg gactcggccg ccggcttcat ttcggctggc tgctctgccg ctcgagaatt
     2581 cgcgggtgac ttgtttattg gcggaggggg aatcgagatc gctgtttcag gaatcggtcc
     2641 cggcgaaggg gcagtgggtc ccggctcatc ggcgaagcac tggcagcttc ccggagtgcc
     2701 cgcgacggga agaagctttc ccgcattcag gaagaattca cccttctggg gaacgatcaa
     2761 tttttcgtcg gaaaattgat tttggagctg gatcgcgcca ctggttgcaa gcacgcaaag
     2821 cgaatcgttg agattcaggc caaccgtcac atcacgcgaa ccgccgttga tatcgagtgg
     2881 tgtgccgatg accgtgggag taaaaacgcg cacggacgtt ttcgaaggta ggctcacccg
     2941 caccgttccg aaattgagcg ctagggtgat ggcgtcgccc gaaagcagca ccgtgaactt
     3001 ggcgggaccg catatgtcga ctgtaccgcc ggcaaaaagg gtcattcgcg ccttcgccga
     3061 gtggacggtg acgacacttc cgttcagaac gtaaatgcta ggcaccgacc gggccgtgcc
     3121 gttattcgcg gcggttccgc tttcaacaga gatgtcgcgg ccctgtacgc ggcccacagc
     3181 gccgggcggc gcatcttggg cacgcgcaat cggagtgagc cacagaaagg taaacgcaag
     3241 aatcgccatt gccgcgcgca gatgcgcgga gcacgttgcg cggtcgggcg cgaattgaaa
     3301 cttgagactc accgggaccg ctatactata cagcaatgga caacgcctcg aactttggcg
     3361 cgttaccaaa gagccacaac cctggcccgg acgtgcgcat tcccatgccg cggctttcgc
     3421 tctggcgaag gatgcaaatt cccgtgttcg cgtgggcagc gtactgggtg atccgcttgc
     3481 tgggtccaac gctgcgcttt gagattgttg gagtgcacaa cgccgtgcaa attcgcgat
//
LOCUS       DATNDT010000360         3018 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4143037, whole genome shotgun sequence.
ACCESSION   DATNDT010000360 DATNDT010000000
VERSION     DATNDT010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4143037"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VN788_11525"
     CDS             complement(<1..260)
                     /locus_tag="VN788_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="motility-associated protein"
                     /protein_id="HXU21203.1"
                     /translation="MFAIVGLLVVLGAVVGGYLIEKGNLTVLIQPAELIIIGGAAIGT
                     VLIANPIHILKKIAAGITGILKGSPFTKQRYLDSLKMMYELFS"
     gene            complement(843..2276)
                     /locus_tag="VN788_11530"
     CDS             complement(843..2276)
                     /locus_tag="VN788_11530"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HXU21204.1"
                     /translation="MPFSDAISKSDSFESERRQSARHAPDSLSYVHLDENNGGILINL
                     SETGLAVQAAVAVMEDDIPRLRLQMPRSKTWLETSARVVWAGDSRRTVGIEFLNSTEE
                     FRQHLREWLAAKGPAVAPPAEAFPLQTESHATNVEEIPLRSIRTSSQPRPRAVDPADY
                     DVAALLASREFASVTVARSMKIPAEHAHAVPAIAATPKEPAPAVSDANQEKPHPKTGT
                     YVPLVIVLALFSLAAGWEAGRGNAFQTVRALFLPSAAANAPKTARPAASAVANGLATN
                     FEVIDSNNQAWLVPFSGPTSAPAGTALPALPAKTAASLNEPPPSPVYTFRTSNLIAPQ
                     LSSRSKAASGSAAPIVTTPQASSLPAAIVDAGESFNLTPPPEPSNAAPNPAVHSSLIE
                     PKLTRTVQPIYPTAALTQHVEGQVKIHARIQADGSLTDVSPVSGPPLLISAAESAVRQ
                     WKYKPEMLDGHAVASDVVVTVQFTAPH"
     gene            complement(2419..>3018)
                     /locus_tag="VN788_11535"
     CDS             complement(2419..>3018)
                     /locus_tag="VN788_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21205.1"
                     /translation="PDGYERTFGGKVRAKMGKKFAIGSLVVVLLAGVGLASGWIMKSQ
                     ESKTPAGVWNGQAIAGSFAGIKVQESASGNVASVSFLYDLDNKSGTDYSLTPDSKLIV
                     MGRLKSTGSLSAESQYHLASSVFLPAGNRTRVMLQTTQPFHWPGQMDAAAEAQFRNMV
                     NHSIADLGGFVIFDPAAHYQIELPGSWPAVADTATVIQN"
BASE COUNT          631 a          863 c          877 g          647 t
ORIGIN      
        1 gaaaaaagtt cgtacatcat tttgagcgaa tcgagataac gctgcttcgt gaagggggag
       61 cctttgagga tgccggtaat cccggccgcg atcttcttca aaatgtgaat cgggttggcg
      121 ataagcaccg tgccgatcgc cgcgccgccg atgataatca actcggccgg ctggatcaga
      181 acggtcagat ttcctttttc gatcaggtat ccgccgacga cggcgcccaa caccaccaac
      241 agccccacaa tcgcgaacat ctaatgtcct ctccaaaagc gaataggctt gctattgctg
      301 gaccgccatt aatgacaccg acacgcaatt tcctcccgtc tacaagcttt tgtcgattgc
      361 agcttaggcg cgctcgatca tcgagccaac gctatctcgt gcacacacga tgagtgcatg
      421 atcgttccga atcggtacaa cgtgcaggcg cacaaaattc tatggcccag cttgtgccgc
      481 gatgaaacat ccatcacgcc tgccttgcca caacacttcg gtcagccctg ccgccgcgcc
      541 tgtcagcacg ttttctgtga tcgtcacttg cgatcaattg gctgccgcaa aacgcgccac
      601 gatctcttgc cgtagatagc ccgtgacttt tttccgcgcc gtcgttccaa gaaacgatcc
      661 gcagttcgcg ttcggcgatg gtttgcgggc tttccagcag tctgcgaaaa atctcggaat
      721 ggtgagattc agacatgcgt ctccctaata gcaacaatcg gtaaaaggga ggccaaggtt
      781 tacaggccaa cgggggtttg gttcttacgg ttgctctgga acttacggcc gaagcgtccg
      841 aatcagtgag gtgccgtaaa ctgcacggtc acaacgacat cgctcgccac tgcatggccg
      901 tcgagcattt cgggtttgta tttccactgg cgaacagcgc tctcggcggc ggaaatcagc
      961 aagggaggac cgctcaccgg gctcacgtcg gtgaggctgc cgtccgcctg gatgcgcgca
     1021 tgaattttta cctggccttc gacgtgttga gtcaaggcgg cagtgggata aattggttgg
     1081 acggtccgag tcagcttcgg ttcaatcagg ctcgaatgca ctgcgggatt gggcgcggca
     1141 ttagatggtt caggaggcgg agtgagattg aaagattctc cggcgtccac gattgcggca
     1201 ggcagggaac tggcttgcgg cgtggtcaca ataggcgcag cgctgccgct tgccgctttt
     1261 gatcgcgaag ataattgcgg agcaataaga ttcgaagtcc tgaacgtata gaccgggctc
     1321 ggaggcggtt cgttcagact cgcggcggtt ttggctggca gcgcgggtaa tgccgtacca
     1381 gctggcgcgg aagtcggtcc gctaaacggc acaagccacg cctgattgtt ggaatcgatc
     1441 acttcaaagt tcgtggcgag tccgttggcg acggcagagg ccgccggccg tgcggtcttg
     1501 ggcgcattcg ccgcggccga cggcaaaaac agcgcacgca cagtttgaaa tgcgtttcca
     1561 cgccccgctt cccatccggc ggcgagggaa aaaagggcta aaacgatcac gaggggaacg
     1621 taagtcccag tcttcggatg cggcttctcc tgattcgcgt cggaaaccgc aggagccggt
     1681 tctttgggcg tcgccgcaat cgcgggcacc gcatgcgcgt gttccgcggg tattttcatc
     1741 gagcgggcta ccgtcacgct ggcgaattcg cgcgacgcca agagcgcggc cacatcatag
     1801 tcggccgggt ccacagcacg tgggcgcggc tgcgaagacg tccgaatgct ccggagcgga
     1861 atctcctcca cgtttgtggc atgactttca gtctgcagag gaaaggcttc ggcaggcggc
     1921 gcgaccgcgg ggccttttgc cgccagccat tcgcgcaaat gttggcgaaa ttcttcggtg
     1981 gagttcagaa actctatgcc gaccgttcgt cgcgaatcgc ccgcccagac tacgcgcgcg
     2041 ctggtttcca gccacgtctt cgaacgcggc atttgcagac gcagccgggg aatatcatct
     2101 tccatcacgg ccaccgccgc ctgcacggct aagccggtct cgctcaggtt gatgaggatg
     2161 ccgccattat tctcgtcgag atgtacgtag gacagcgaat cgggcgcatg ccgggcggat
     2221 tggcggcgtt cactttcgaa tgaatcgctc ttcgatatcg cgtcgctaaa aggcatttag
     2281 tttccgagga agtcccaatt tgatccggag caattacagg tccaaggagg aaggaactat
     2341 acgccttcct ttcgcccatg ggaagtgcag aacctcttcg gcgccaacgg gagcgctaac
     2401 accgggctct ttgagccgct aattctggat gaccgtggcg gtgtcggcta cggccggcca
     2461 ggagcccggt aattcaattt ggtaatgagc ggccggatca aagatcacga aaccgccaag
     2521 gtctgcgatc gagtgattca ccatgttgcg gaactgtgcc tcagcggccg catccatctg
     2581 gccgggccaa tggaacggct gcgtcgtttg gagcatcacg cgagtgcgat tcccagcagg
     2641 caaaaatact gacgatgcga gatggtattg gctttccgca ctcaggctgc cggttgactt
     2701 taatcgtccc atcacgatca gcttcgaatc gggcgtcaga ctgtaatcgg tgcccgattt
     2761 gttatcgaga tcgtagagga aggatacgga ggcgacgttg cccgaagccg actcttgtac
     2821 cttaatgccg gcaaacgaac cggcaatggc ttgcccgttc cacacgcccg caggcgtctt
     2881 ggactcctgc gacttcatga tccatccgct tgctagccct acgccggcca gcagaacgac
     2941 aactaggctt ccaatggcaa atttttttcc catttttgcc ctgactttac cgccgaaggt
     3001 tcgttcatac ccatccgg
//
LOCUS       DATNDT010000361         4268 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3319852, whole genome shotgun sequence.
ACCESSION   DATNDT010000361 DATNDT010000000
VERSION     DATNDT010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4268
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3319852"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="VN788_11540"
     CDS             <1..969
                     /locus_tag="VN788_11540"
                     /inference="COORDINATES: protein motif:HMM:NF014680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXU21206.1"
                     /translation="ATQGQNMSAQINHLVQTMTQQLGQVRQDVQTGVASSGQLATDAQ
                     REVAQRLESSTQALVEMSRKIGEMHQTSQDLSKTTSMLQGILSGVKTRGALGETMLKT
                     LLADALPQNAYETQHRFASTGCIVDAIVRSGERMLCIDSKFPIEAYRRLVEKGDEARR
                     DFSVAVRKHADSIAEKYILPQEHTLDYALMFVPSEGVYYELVMAEDSKYGRLEEYCRG
                     KRVFPVSPNTFCAFLGAVAMSLQGQKLEENVRHLLASLSGLKQQMDTFAGIHEKLGTH
                     LRYVQQNYEEADSKLNRLRNSLEQMSQGALIEALPKALETVPTEQD"
     gene            complement(999..2093)
                     /locus_tag="VN788_11545"
     CDS             complement(999..2093)
                     /locus_tag="VN788_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21207.1"
                     /translation="MRIAPRLPRYSQLSAIRRIIVNVILGLTLFSGILFSGTRASQNE
                     PSVSNAWMVTIVLPPELMAGHPATLAVLGVDGKLAAGVKVDMGNDLSVTTDRTGRALF
                     TVPSSGDYLLAKASGASAAALIDPAAGASEPKAVSLPPVVSVRDRFWICGAGLSSEAD
                     ANSVLINGKAAVVLAASPVCVVVLAEPNSATGPTSILVQAPGVRWDAATTLVSLQFVA
                     PSPALQPGQKGKLSVRVDGSAQPLRLAVEDRNPGVLRFESGGVRRLTTSGGTTNVAEI
                     KVQAITSGDYSLSCRLMPIPNVPTARRFLIAAEALAPEDSRRRISGLVSRLARNPGAA
                     EKFEPDLDQIATRTMAGDLRTLLDASRDAL"
     gene            complement(2100..2939)
                     /locus_tag="VN788_11550"
     CDS             complement(2100..2939)
                     /locus_tag="VN788_11550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HXU21208.1"
                     /translation="MQTNQESDRSVNVFSGPADFGCGRYGIYPGCRNVFSWQVALLGL
                     SVMLVAGCARRTYTAHAPAQPPSGRLPSAPGESTPSGPSAAPPSVERQPAIPGEYVEE
                     GVASWYGVPFDGHRTSDGEIYHMHQFTAAHRTLPFGSIVRVTNLDNGKQTQVRINDRG
                     PFVADRVIDLSLSAAQAIGMVGPGTAKVRLEIVGGPPNPAPGVFGVQVGAFETQSSAE
                     RLRGQLEARYAPVIVVAYDSPKGKFYRVRVGSFRTQDAARQFAGQLRSSDRFTTFVVR
                     LDD"
     gene            3147..3977
                     /locus_tag="VN788_11555"
     CDS             3147..3977
                     /locus_tag="VN788_11555"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HXU21209.1"
                     /translation="MRFSLAAASRSLGGLMPAQSPATYGILVLSCVFFVISLLATLRT
                     TGFASPSGGGLSGLFNFGAISGRVLQRFGASLPLPYNLAQPWRFVMAIFLHGSILHIF
                     FNMWVLMDIGPQIEELYGSARYFFIYIVTGIGGYVVSCMTGHMSIGGSGALLGLIGVL
                     LAVTTGRKSAGMKMLRNQMIRWLIYIAVWGLLFPGIDNYAHAGGLISGFVLGKIMADR
                     APASPQEQKRAYALGWGAALIVAASIALMILANVRAGAPARASTAYCPVCSSRETTML
                     "
     gene            complement(3935..>4268)
                     /locus_tag="VN788_11560"
     CDS             complement(3935..>4268)
                     /locus_tag="VN788_11560"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HXU21210.1"
                     /translation="LSSSYLVLALKTKIAWWLAIMVLGRDVLLLVACAVILLTVGYRP
                     FPPSIWGKATTFFEIFLIVLVLVLAVWFNEGLWLVREFSGYIVGALVIFSGLHYSIVV
                     SRELQTGQ"
BASE COUNT          963 a         1241 c         1233 g          831 t
ORIGIN      
        1 gcgacgcagg gccaaaatat gtccgctcag atcaaccatc tcgtgcaaac catgacccag
       61 cagcttggtc aagtccgcca ggatgtgcaa acgggagtcg cctcgagcgg ccagctcgca
      121 accgacgcgc agagggaagt agcgcagcgg ctcgaatcct ctacgcaggc tttggtggag
      181 atgagccgaa aaatcggcga gatgcaccag accagccagg atctctcgaa gactacgtcc
      241 atgctccaag gaattctgag cggagtgaaa acgcgcgggg cgctgggcga aaccatgctg
      301 aaaacgctgc tcgcggacgc gctgccgcaa aacgcatatg aaacgcagca tcgttttgca
      361 tccacaggtt gcatcgtgga cgccatcgtg cggagcggcg aacgcatgct gtgcattgat
      421 tcaaaatttc cgattgaggc ataccggcga ttggtggaaa agggcgacga agcgcggcgc
      481 gatttttcgg ttgccgttcg caagcacgcc gattccatcg ccgaaaagta cattcttccg
      541 caagagcaca cactcgatta cgcgctgatg ttcgtgccat ccgagggcgt gtattacgag
      601 ctggtgatgg ccgaagattc gaaatacggg cgactggaag aatactgtcg cggcaaacgc
      661 gtattccccg tatcgccaaa tactttctgc gccttcttag gcgctgttgc gatgagcctt
      721 cagggccaga agctggaaga aaatgtgcgg cacctgcttg cttcgctatc gggcctaaaa
      781 caacaaatgg acacatttgc gggcatccat gaaaaacttg gaacgcatct tcgttacgtt
      841 cagcagaact atgaggaggc cgacagcaaa ttgaatcgcc tgcggaactc gctcgagcaa
      901 atgtctcagg gggctttgat cgaagcgctg ccgaaagcgc tcgaaaccgt tccaaccgag
      961 caagactgag cggcccagtc gcatcccaga actagccctc agagagcgtc gcgcgacgcg
     1021 tccagcagcg tgcgcaaatc cccagccatt gttcgcgtgg caatttgatc gagatcaggc
     1081 tcgaactttt cagcagcgcc ggggttgcgt gccaaacgag agactagtcc ggatattctg
     1141 cggcgcgaat cctcgggggc cagggcttcg gctgcgatga gaaatcttcg cgcggtggga
     1201 acgttgggga tcggcataag acgacagctt agtgagtaat cgcctgaggt gatcgcctgc
     1261 acctttattt ccgcgacatt cgtggtccct cccgacgtgg tcagccgccg cacgcctcca
     1321 ctttcgaatc gaaggacccc tggattccta tcttctacag caagccttag aggttgggcg
     1381 gagccatcga cacgaacgga aagctttccc ttttgtccag gttgaagcgc cggactcggt
     1441 gccacgaatt ggagagacac gagcgtggtc gcggcgtccc agcgaacgcc gggcgcctgt
     1501 accaaaatag aggtcggccc ggttgcggag ttcggctctg caagcacgac cacacaaacc
     1561 ggtgacgccg cgagcacaac tgcagctttg ccatttatga gcacgctatt tgcgtcggcc
     1621 tcgctactca atccagctcc gcaaatccaa aagcgatcgc gcacggaaac cacaggtggc
     1681 agggaaacag ctttgggttc gctcgcgccc gcagccggat cgatcagcgc cgccgcggaa
     1741 gcgccggaag ccttcgccaa aagataatca ccggaggacg gtacggtaaa aagagcacgg
     1801 ccggtgcgat cggttgtgac actcaggtca ttgcccatat ccactttcac tccggccgcc
     1861 aattttccgt ccacaccgag aacagcaagg gtcgctggat ggccggccat gagttccggc
     1921 gggagtacga tggtaaccat ccaggcgttg gaaacactcg gctcgttttg cgacgcacgg
     1981 gtcccgctga agagaatgcc gctgaagagg gtcagtccaa ggattacatt tacgatgatt
     2041 ctgcgaatgg cggaaagctg cgaatatcgc gggaggcgag gcgcgattct cataactcgt
     2101 caatcgtcca agcgcaccac gaaagtcgtg aagcggtccg aggagcgcaa ttgcccggcg
     2161 aattggcggg cggcatcctg ggtgcgaaaa cttccgaccc gaacacgata gaacttgccc
     2221 tttggcgaat cgtacgcgac gacgatgacc ggggcgtacc gcgcctcgag ctgcccgcgc
     2281 agccgctcag cgctgctttg cgtctcaaac gcgcccactt gaacgccaaa aacgccaggc
     2341 gccggattcg gtggcccccc aacaatctcc aagcggacct tggcggtgcc tggaccgacc
     2401 atcccaatcg cctgcgcggc tgaaagggat aggtcgatca ctcgatccgc gacaaatgga
     2461 ccgcggtcgt tgattcggac ttgcgtctgc tttccattat ctaagtttgt cacccgtacg
     2521 atcgagccaa atggcagagt gcggtgcgca gccgtgaatt gatgcatgtg ataaatttcg
     2581 ccatccgaag tgcgatggcc gtcgaagggt acgccatacc acgaggccac tccctcttca
     2641 acatattctc ccggaattgc cggctgcctc tcgaccgaag gtggcgcggc ggaaggaccc
     2701 gaaggcgtac tctcgccagg ggcggaggga agtcgtccgg aaggcggttg agccggggcg
     2761 tgcgccgtgt aggtccgacg cgcgcaaccc gccactagca tcactgaaag ccccagaagc
     2821 gccacttgcc acgagaaaac atttcggcag ccaggataaa ttccataccg cccgcagccg
     2881 aagtccgctg ggcccgaaaa tacgttcacg ctgcgatctg attcctgatt tgtttgcacg
     2941 taatttcggt attataggag tgcgttcgca tcctcgcaag gaaagccatt gatatcgtta
     3001 tccctccttc accggtggct tgatcggact tcaaaagaat gagcggaatt tttggacgcg
     3061 gcagacaggg ccgcccccaa atctgtccag cttgccggtc gctcgtcggc gttggggcca
     3121 cgcactgcca ccaatgcggc gcgaacttga gattctctct cgcggcagcc agccgctcgc
     3181 tgggcggctt gatgccggcg cagtctcccg ctacgtatgg aatcctggtg ctgagctgcg
     3241 tattcttcgt catcagtttg ctggccaccc ttcgcacaac aggcttcgcg tcaccgagcg
     3301 gcggcgggct ctctggcctt ttcaactttg gcgctatcag tggcagggtg ctacaacgct
     3361 tcggtgcctc gctgccgctg ccgtacaacc ttgcgcagcc gtggcgattc gtcatggcga
     3421 tttttcttca cggcagtatt ctgcacattt ttttcaatat gtgggtgttg atggatattg
     3481 gcccgcaaat cgaagagctg tacggatcgg cgcgctactt cttcatttac attgtcaccg
     3541 ggattggagg atatgtggtg agctgcatga caggccacat gagcatcggg ggatccggtg
     3601 cgctgcttgg gctgatcggc gtgcttcttg ccgtaacaac cggccgtaag agcgcaggaa
     3661 tgaagatgct tcgaaatcag atgatcagat ggctcattta catcgccgtt tggggactat
     3721 tattcccggg aattgataat tacgcacatg cgggcggcct aatcagcggt ttcgtcctgg
     3781 gcaaaatcat ggcggatcgg gcgccagcat ctccccaaga acagaagcga gcgtacgcgc
     3841 taggctgggg cgcggcgtta attgtcgccg ccagcatcgc gctgatgatt ctggccaacg
     3901 tgcgggctgg cgcgcctgcc cgcgccagca cggcctattg ccccgtctgt agctcgcgcg
     3961 aaaccacgat gctgtagtgc aatccagaga aaatcaccaa cgctccaaca atatagccgc
     4021 tgaattcccg cacgagccat aggccttcgt taaaccagac ggccaacact agaaccaaca
     4081 cgataagaaa aatctcaaaa aacgtggtcg ctttccccca aatgctgggc ggaaagggcc
     4141 ggtagccaac cgtaagaaga atcaccgcac acgccaccag aagcagaacg tcgcgcccga
     4201 gaaccatgat cgccagccac caggcgattt tcgttttcag cgccaaaacc agataagagg
     4261 aagaaagc
//
LOCUS       DATNDT010000362        10187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_108263, whole genome shotgun sequence.
ACCESSION   DATNDT010000362 DATNDT010000000
VERSION     DATNDT010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10187
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108263"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(491..901)
                     /locus_tag="VN788_11565"
     CDS             complement(491..901)
                     /locus_tag="VN788_11565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21211.1"
                     /translation="MHAEQNDLLKRVSDLENQVANLKAQVTSLEEQKKSPDFYARYKE
                     EAATRDGRELVLPSVCKVNAVMETAAEVRRYGMTFGTADYEFQHLSDTGCCCSGVDQF
                     PGFENYFKHQIGFAVRKSKGKKITYSAIQNEWGS"
     gene            complement(935..3448)
                     /gene="mgtA"
                     /locus_tag="VN788_11570"
     CDS             complement(935..3448)
                     /gene="mgtA"
                     /locus_tag="VN788_11570"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006871448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HXU21212.1"
                     /translation="MNDSAVPRCEQLPPTMTQGLSSEEAAARLAEYGPNDATPKKHAE
                     TALELLRLFLNPLVSILLIASLASFLLGDKADATLILVIVLLSVSINFVQTYRSEHAI
                     LNLREKVTLTATVIRDGKWTEIKRREVVPGDVVRVSAGDLIPADGQLVESRDLYVQQA
                     ALTGESLPAEKDARGINNSNAARGPDAPDLVFFGTSVVSGTGTIVILRTGSKTEFGAI
                     AERLSARPEETEFEHSMKHFGMLITRAVFFLVLFIVIVRMAMHKDAFESFVFAVALAV
                     GLTPEFLPMISSVTLARGAMRMARDQVVVKHLSAIQNFGTIDVLCSDKTGTLTMGQML
                     LAASVDCLGAKSDRPLELGYLNSKFETGIRSPLDSAILSTEHPNKEIYTKCDELPFDF
                     DRRRLSILLEHKDTHRRLLIAKGAPEGILGICESCEVQSKSVPLDADTRKRARQTFDD
                     LSEQGFRVLAIAYREVKEQTSFTTADERSLVLAGYLSFADPPHPDAAASLAAMKRDGV
                     EIKLLTGDNELVARHVCEQVGLRNPAIILGDQLEKMTDPALQHLADKIAVFARVSPMQ
                     KHRIIHALKHRGHVVGYMGDGINDAPSLRAADVGISVASAVDVAREAADIILLKPGLE
                     VLHSGIIEGRRASANVMKYLLMGTSSNFGNMFSMAGASTFLPFLPMLPTQILLNNFLY
                     DAAQITIPTDRVDDQYIRRPRRWDMKLIRNFMIFIGPISSIYDFLTFYVLLHFFRAGQ
                     SEFHTGWFVESLATQTLVLFVIRTMGNPLKSRPSTPLTVTTVLIVIIGVLLPYSPLAG
                     LLGFTKLPAAYFTFLAISTVTYLLLVEFAKRLLFSEKER"
     gene            complement(3685..4293)
                     /locus_tag="VN788_11575"
     CDS             complement(3685..4293)
                     /locus_tag="VN788_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21213.1"
                     /translation="MSFARAISGNISLSRTSSLTPPQKEQDGSGERLVRLHWQHLRSF
                     ASSPRTSITVLIDSILDLRKGLINSENSTTIAAKQQANPCDCEIFRLLGDLWRKLTLQ
                     ICSAKSRQVLGNKRDSVVKFLGHPRLNRFDQPLRPNKENQKEFLPNAEVSCMFRNFAA
                     QVDILRIHALLECVALSSVWHRRRASALPEPPDIRRPPESIS"
     gene            4383..6146
                     /locus_tag="VN788_11580"
     CDS             4383..6146
                     /locus_tag="VN788_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018863971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HXU21214.1"
                     /translation="MKFRALALDYDGTIAEDGVLNPDVRAAIAEVRACGTVVVIVTGR
                     TISDLKRVVGNLDFIDAVVAENGAVLELPNRYSMLIGAPPPAVFFEELTRRGIKVQAG
                     QCVVESDGAFAPQFLEVIRQLELPLVLLFNRSRLMILPQGVSKSVGLHRALSALHLSS
                     HNAIAIGDAENDHDLLASCELGVAVGWGSSALKHAADEVLEGEGPDAVAEFIRKTCHE
                     MRLAPSRIGRYRVPLGTSEDGRAVALSIRGRNALVSGDPRSGKSWVTGLACEKMILQG
                     YCVCVIDPEGDYRTLESLPGVILLGGDDPPPQLPDLARILRHPEMSVVIDLSHVPYKE
                     KIDYLHSLLPMLAAIRRSTGLPHRIVVDEAHYFLHDRNAKQMLDLSLNAYTLVTYRIS
                     DLNNELRKTIEANVVTRTTDPREVRALAAMAENESAAAEWASVLGGLSVNQAVLLPGT
                     EEAEGKLIRFDLLPRLTAHVRHKAKYLDVRLMDRQAFVFTQNDTPWGPPARTLEEFIE
                     LLKSAPAAVLEGHAHRADFSRWIAEVFHDRTLAATIRKTEQRYRLGHVQDLTNSLAAA
                     VRERYEFSSTIPSEAEPNVVW"
     gene            6308..8107
                     /locus_tag="VN788_11585"
     CDS             6308..8107
                     /locus_tag="VN788_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HXU21215.1"
                     /translation="MDRRFDAGNQEIAPAEELFRALVASVKDYAIFMLNPQGQVSTWN
                     PGAECIKGYSAEEIIGQHFSRFYTAEDIEQGKPDRELKIAAAEDRFEEEGWRVRKDGS
                     RFWASVIITAIRDDKGELLGFSKVTRDFTERKKVEDALHVSEERFRVLFEFSPDAIIV
                     VDEQGKIAEVNAQVEKFFGYKPSELSGQTVDILVPERFRNVHPHHREQYRAHARTRPM
                     GIGLELRGRRKDGTEFPVDIMLSPVDTTEGKVIVSVIRDLSEKMRAQEELERKDREKR
                     YLQEELHVENRFEDIIGESPVLKRALKEVETVAATDVTVLILGETGTGKDLVARAIHQ
                     LSGRREGNLVKLNCAAIPTGLLESELFGHERGAFTGAISQKIGRLELAHQGTFFLDEV
                     GDLPLELQPKLLRALQEKEFERLGGTRTIPVNARLIAATNRDLGKMVNDRQFRSDLFY
                     RLRVFPINLPPLRERREDIPLLVRYFVSKHARTLYKAIETIPSDIMKALTRWEWPGNI
                     RELENFVERSVILSKGPALWAPLAELELPSEYPSSESSTLESTEKDHILKILRETEGV
                     IAGPQGAAARLGLKRTTLNSKLRKLGIRRSDFM"
     gene            8282..8587
                     /locus_tag="VN788_11590"
     CDS             8282..8587
                     /locus_tag="VN788_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21216.1"
                     /translation="MTLRITVNESPESTTMKLEGKLIGPWVAELDRAWRSLLVSLSSK
                     KLRVDLCGIVRIDPNGRQILAEIHRQTGAEFVADTPITKYFAEQARLQGQKDSSEGE"
     gene            8589..9305
                     /locus_tag="VN788_11595"
     CDS             8589..9305
                     /locus_tag="VN788_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU21217.1"
                     /translation="MRGPYGFEMSENCQTSKLKKCGFFCDLTPKAVKDFDSIKSTSAF
                     PDGAVLFMEKQDPRGVFVLCQGEVKLTISSSEGKTLILRIAKAGEILGLMSVLAGKPY
                     EVTAETLRPSQIAFIRREDFLRFVAQHPEVSAGIVKQLSGNYHTACEQLRTVGLSTSA
                     RGRLAKLLLDWAAGAEQTKLGTRIQIPLTHEEIAECVGSSRETVTRTFSGFKNQNLVS
                     IKGSSLLIINRAGLESFVAT"
     gene            9473..9796
                     /locus_tag="VN788_11600"
     CDS             9473..9796
                     /locus_tag="VN788_11600"
                     /inference="COORDINATES: protein motif:HMM:NF017736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HXU21218.1"
                     /translation="MLIALLVSTHSLPASPLQEHRLQRYHTHTGERLDIVYRRGGTYV
                     SDAIAKLDYFLCDHRTGDVRHFDPKIFDALEDLTIAVGHPDLTVTGPGIFDPPEGLVS
                     GKIHA"
     gene            9932..10187
                     /locus_tag="VN788_11605"
                     /pseudo
     CDS             9932..10187
                     /locus_tag="VN788_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612267.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
BASE COUNT         2334 a         2728 c         2860 g         2265 t
ORIGIN      
        1 tcaatccggt tgtcactcgt gccgcagaaa ttagcgtacg aaccggaaca tcgactggat
       61 ctaccccttt ttgcccccgc gtgcttttaa taagttccta ccgttcaaag aaatgattgg
      121 taggggcggt ggggatcgaa ctaaaagcgg cgttgaagat gcgtaagttg ttgattctat
      181 taaacgagaa aaacgccaaa aacaccatat tcgcccaacc gaggtacacg ccgggcacac
      241 ggagacagaa agcggaaagc aatcaaatcg gtttcactca gtggggcgat cccggaccga
      301 gtcgtcccac tcgtagataa ccatgcctcg ctcgttgtcg cgagatggcc tgacgccaaa
      361 aaacgacgag gggctaccag gagcaaatgg ttgattccac ctcagcttaa cgtggtcctt
      421 caatgtcgcc ttattgcaat ttcgtgctgc gatccgggac tttgaattca agaatctatc
      481 gaccgaaccc ttaggagccc cattcgttct ggatggctga gtacgttatc tttttcccct
      541 tcgacttacg aaccgcaaaa cctatctgat gcttgaagta gttctcaaag cccggaaatt
      601 gatcgacccc gctacagcag cagcccgtat cagaaagatg ctgaaattcg tagtcagcag
      661 ttccaaacgt cattccatat cgtcgcactt cagccgctgt ttccatgacg gcattcacct
      721 tgcaaactga aggaagcacc aactctctgc cgtcgcgtgt cgcagcttct tccttgtatc
      781 gtgcgtagaa gtctggcgac tttttctgct cttctaatga ggttacttgt gctttgagat
      841 tggcaacctg attttcaaga tcgctgaccc ttttcaataa atcgttctgc tcggcgtgca
      901 ccgcctggga gtggttccgt cagaaagttg agggtcaacg ctctttttcg gagaagagca
      961 gacgttttgc aaattcgacc agcagaagat aggttacggt cgagatcgca aggaaggtga
     1021 agtatgcagc cggaagcttc gtgaaaccaa gaagtccggc aagcggggaa tagggcagta
     1081 agactccgat aatcacgatc agcaccgtcg tgaccgtaag aggagtactc ggccgactct
     1141 ttagcggatt gcccatggtc cgaatgacaa agagcacaag cgtctgcgtg gcgagtgact
     1201 cgacgaacca cccggtatga aattccgact ggcccgcgcg gaagaaatgg agcagcacat
     1261 aaaatgtcag aaaatcgtag atcgagctga ttggaccgat gaaaatcata aagttgcgga
     1321 tcaacttcat atcccatcgg cgaggccggc gaatgtattg gtcatcgacc ctgtcggtcg
     1381 gaattgtgat ctgcgcggcg tcatagagga agttgttcaa gaggatctgc gtagggagca
     1441 tcggaagaaa cggcaagaat gtcgatgctc cggccatact gaacatgtta ccgaagttgg
     1501 aactcgtgcc catgagcagg tacttcatca cattcgccga tgcacggcgg ccttcgatga
     1561 tgccgctgtg caagacctca agaccgggtt ttagcagaat gatgtccgct gcttcccggg
     1621 cgacatctac cgcggaagcc acggaaatgc cgacgtcggc ggcgcgtagc gagggtgcat
     1681 cgttgatgcc gtctcccata tagccgacca cgtgtccacg gtgcttcagc gcatggatga
     1741 tgcgatgctt ttgcatcggc gagactcttg cgaacaccgc gattttgtcg gccagatgct
     1801 ggagcgccgg gtcagtcatc ttctcgagtt gatcgccgag gataatcgct ggatttctca
     1861 atcccacctg ttcgcagacg tgccgcgcga caagctcgtt gtctccagtc aggagtttta
     1921 tctccacgcc atctcgcttc atggccgcaa gagacgcagc cgcatccggg tggggcgggt
     1981 cggcgaatga aagatagccg gccagcacga gcgatcgttc gtctgcggtc gtaaagctag
     2041 tctgctcctt gacttcgcga taggcgatgg caagcactcg aaatccctgt tcgctgagat
     2101 catcgaaggt ctgtcgtgca cgcttacggg tgtctgcgtc gagagggacg gatttactct
     2161 ggacctcaca ggattcacat attcccagga ttccctcggg cgctcctttt gctatcagca
     2221 gccggcggtg agtatcttta tgctctagaa ggatagaaag tcgacggcga tcaaaatcga
     2281 acggcagttc atcgcatttg gtgtagatct ctttgttggg gtgctccgtg cttagaatcg
     2341 cgctatcgag cgggctgcga atccctgttt caaatttgct gttcaaatac cccagttcga
     2401 gaggacggtc ggatttcgca cccaagcaat ctacggaggc cgcaagaagc atttgaccca
     2461 tcgtaagagt gccggtcttg tcgctgcaaa gaacatcaat cgtgccgaag ttttgaatgg
     2521 cggaaaggtg tttcaccacg acttggtcgc gtgccatccg cattgccccg cgcgcaagcg
     2581 tgaccgaact gatcatggga aggaattcgg gcgtgaggcc caccgcaagg gccacggcaa
     2641 aaacaaagga ttcgaacgcg tccttgtgca tcgccattcg cactatcaca atgaaaagga
     2701 ccaggaaaaa tacggcgcga gttatcagca tcccgaagtg tttcatgctg tgttcgaatt
     2761 ccgtttcctc cggtctcgcg gacaggcgtt cggcaatcgc gccgaattcc gttttcgatc
     2821 cggtcctaag gatcacgatc gttccggtcc cgctgacaac agaggttccg aagaacacca
     2881 gatcgggcgc gtcgggtccg cgcgcagcgt tgctgttatt tatgcctcgc gcatcctttt
     2941 ccgcaggcag agactcgccg gtcaatgccg cctgctgaac atataggtcg cgcgactcga
     3001 caagttgccc gtcggcggga atgaggtcac ccgccgagac gcgcacgacg tcgcctggaa
     3061 cgacttcccg acgtttgatc tcagtccatt tcccgtcacg aatgacggta gccgtgagtg
     3121 tgactttctc ccgaagattc aagatggcgt gctcggaacg ataggtctga acaaagttga
     3181 tggagacgct taagagcacg atcaccaaaa tgagagtggc gtctgcttta tctcccagga
     3241 gaaacgaagc caggctggca atgagcagga ttgagacaag cgggttcaag aacagtcgca
     3301 gcaactcaag ggcggtctcc gcgtgcttct tgggtgtcgc gtcattcggg ccatactctg
     3361 ccaaccgtgc agctgcttct tcggaagaga ggccctgcgt catggtcggc ggcagttgtt
     3421 cacagcgagg cactgcggag tcattcatgt ttgtgccgga cgaccttgca aatgcttttt
     3481 gacagcggtg cgctggtgtt ctctgcccta tctcgcgcca ggtcttatga acaacttcgt
     3541 ttaaggcaaa tgtctttgtt tatatcggca aggatatgtt gttccgtcgc tccgcacaaa
     3601 tgtttgccgg gatgcttgtc cgaatcggtg atctacatca catgtccacg tgatgcgctg
     3661 ccttacgccc gacaggcaag cgggctaact tatcgattcc ggcggtcgtc ggatgtctgg
     3721 cggctcgggc agcgcggacg cccgtcgccg atgccacacg gagctcaacg cgacgcattc
     3781 gagcagcgcg tggatgcgca gtatgtccac ctgtgcagcg aagttgcgga acatacaaga
     3841 aacctctgcg ttcgggagaa actctttttg attttccttg ttgggtcgaa gcggctgatc
     3901 gaaccgattc agtcggggat gtcccaggaa cttaacgacc gaatcacgct tattcccgag
     3961 gacttggcga gacttagcgc tgcagatttg cagcgtcagc ttgcgccaaa gatcgccgag
     4021 caggcgaaag atttcgcagt cgcacggatt cgcctgctgc ttagcggcga tcgtagtact
     4081 gttctcggaa ttgatgagac ccttgcgcaa atccagaatg gagtcgatca gtacggtaat
     4141 gctcgtccgc ggactcgacg caaagctgcg caagtgttgc caatgcagcc tgaccagtcg
     4201 ttcaccggac ccatcctgct ctttctgcgg tggcgtaaga ctggacgtcc tactgagcga
     4261 gatattgccg gagatagcgc gtgcgaacga cacagaaatg caagtcgtgg aaggcgaaat
     4321 cgcggggccg ttgacggaat ccggcggaat gggcggttgg ctgcgcagac cgaggcaggc
     4381 ttatgaagtt ccgagcacta gcgctcgact atgacggaac gatcgctgaa gacggagttc
     4441 tgaatccgga cgttagagcc gcgatcgccg aggttcgcgc atgcgggacg gtggtcgtaa
     4501 tcgtaaccgg gcgaacgatc tccgacctga agcgtgtcgt cggaaatctg gatttcatag
     4561 acgctgtcgt agccgaaaac ggtgcggttc tagagctccc gaatcgttat tccatgctaa
     4621 ttggtgctcc gcctcccgcc gtgttcttcg aggaactcac ccgacgtgga atcaaagtac
     4681 aggccgggca gtgcgttgtg gagtcggatg gcgccttcgc gccacagttc ttggaagtca
     4741 tccggcaact tgaacttcct ctcgtgctgc tgttcaaccg tagccgcctg atgattcttc
     4801 cacaaggcgt cagcaaaagt gtcgggctgc atcgcgcgct cagtgctctg cacttatcgt
     4861 ctcacaacgc gattgcaatc ggcgatgcgg agaatgacca cgatctcttg gcttcgtgcg
     4921 aactcggcgt agctgttggt tggggtagtt ctgccctgaa gcacgccgca gacgaggttc
     4981 ttgaaggaga aggccccgac gccgtggccg agtttattag aaaaacgtgt cacgaaatga
     5041 gattagcgcc cagccgaatt ggacgatatc gagtgccttt aggaacctcg gaagatggcc
     5101 gggcggtcgc gctgtcaata cgcggccgta acgctcttgt ttctggtgac ccgcgttcag
     5161 gaaagtcttg ggtaacggga ctcgcgtgcg aaaagatgat tttacaaggg tattgtgttt
     5221 gcgtgatcga tcctgaggga gactaccgca cgcttgagtc gcttccagga gtcatactcc
     5281 tcggaggtga tgatcctcct ccccaactgc ccgacctcgc acgcatactg cgtcaccccg
     5341 agatgagtgt ggtgattgat ctctcgcatg tgccatacaa agagaagatc gactaccttc
     5401 attcgctgct gccaatgctg gcggccatac gacgatctac cggtctcccg caccggattg
     5461 tcgtagacga agctcactac tttctgcacg accgaaatgc gaagcagatg ctcgatctca
     5521 gcctgaatgc ctatacgttg gttacttacc gcatctcaga tcttaacaat gaactccgaa
     5581 agaccatcga ggcaaatgtc gtgactcgta caaccgatcc gcgagaggtg cgggcgctcg
     5641 cggcgatggc ggagaacgaa agcgcggccg cggaatgggc gtccgtgcta ggcggtctct
     5701 ccgtgaatca ggccgttctc ctcccaggta ccgaggaggc ggaaggaaag ctgattcgat
     5761 tcgatctcct tccgcgcctc accgcgcacg ttcgtcacaa ggccaagtat cttgatgtcc
     5821 gactgatgga tcggcaggct ttcgtcttta cgcaaaacga cacaccatgg gggccaccgg
     5881 cgcgaaccct ggaggagttc attgagctcc tcaagagcgc tccggctgcc gttctcgaag
     5941 gtcacgctca ccgcgccgat ttttcccgct ggatcgccga ggtttttcat gatcgcacgt
     6001 tggccgccac cattcggaag accgagcagc gctatcgtct gggccacgta caggatctaa
     6061 ccaattcgct agcggccgct gtccgcgaac gctacgagtt ctcttcaaca atcccatcgg
     6121 aagctgaacc gaatgtcgtt tggtgaccac gcgggttgac gcccctcccc agcggtgatt
     6181 cactcgcgtg cgctattttt cgagtatact gacagtctaa ccgcgagagc atcggcgtcg
     6241 ccgtggtggc gatgtcgctg tttcctgaag gtctgacacg aaatctcttc gtgccggggt
     6301 tcaagacgtg gaccggagat tcgacgctgg aaatcaggag atcgcacccg ctgaagagct
     6361 atttcgagct ttggttgcga gtgtaaagga ttatgcaatc ttcatgctta atccgcaggg
     6421 gcaggtttcc acgtggaatc caggggccga gtgcatcaag ggttacagcg ccgaggaaat
     6481 catcggccag cacttttccc gcttttacac cgcagaggac atcgagcagg gcaagcccga
     6541 tcgcgagctg aagatagccg ccgccgagga tcgattcgaa gaggaaggat ggcgagtccg
     6601 caaagatggg tcccgctttt gggcaagcgt catcatcacg gccatccgcg acgataaggg
     6661 cgaattgctc ggtttctcga aggtgacgcg tgatttcacc gaacgcaaga aggttgagga
     6721 tgcgctgcac gtcagcgaag aacgctttcg tgttcttttc gaattctcac cggatgcgat
     6781 tatcgtagtc gacgagcagg gaaaaatcgc cgaagttaac gcgcaagtcg aaaaattctt
     6841 cggctacaag ccttcggaac tgagtggcca gaccgtcgat atcttggttc ccgagcgatt
     6901 ccgcaatgtt cacccacatc accgcgagca gtatagagct cacgcccgta ctcgcccgat
     6961 gggcatagga ttagagcttc ggggtagacg caaggacggg accgaattcc cggtggacat
     7021 catgctcagc ccggtagaca ccacagaggg caaggtcatc gtcagcgtaa tccgtgacct
     7081 ttcggaaaag atgcgcgccc aggaggaatt ggaacgaaaa gatcgtgaga agcgttattt
     7141 gcaggaagaa ctccacgtag agaaccgatt tgaagacatt atcggtgaaa gtcctgttct
     7201 gaagcgggcc ttgaaagaag tagaaaccgt agcggcgacg gacgtaacgg tcttgatctt
     7261 gggcgaaacc ggaacgggta aggaccttgt tgcccgggcc atccaccaat tgagcggccg
     7321 ccgtgaagga aacctcgtca aattgaactg tgctgcaatc cccacaggcc ttctcgaaag
     7381 cgagcttttc ggccatgagc ggggagcgtt caccggcgcc atctctcaga agatcggtcg
     7441 tctggagctc gctcaccagg gtactttttt cctggatgag gttggggacc tcccgttgga
     7501 actgcaacct aagctgttgc gcgctctcca ggaaaaagag ttcgaacgcc tgggcggaac
     7561 gcgcacgatt cccgtcaatg cgcgcttgat cgctgcgacc aatcgcgatc tagggaagat
     7621 ggtcaatgat cggcaattcc gcagcgatct cttttatcgt ctgagagtct ttccgatcaa
     7681 tctgcctccc ctccgtgagc gtcgcgaaga catccctctt ttggttcgct attttgtaag
     7741 caagcacgcg cgaactctat acaaggcgat cgaaacgatc ccgtcggaca tcatgaaggc
     7801 cctaactcgt tgggaatggc cgggaaatat tcgagaactt gagaacttcg tcgaacggtc
     7861 cgtcattttg agcaaaggcc ccgctctttg ggcgccgctc gcggagctag agctcccgag
     7921 cgaatatccc tccagcgaga gttcaacgct tgaatcgacc gaaaaagacc acatcctgaa
     7981 aatcctgcgc gaaaccgaag gcgtcatcgc cggacctcaa ggagcggctg cgcgccttgg
     8041 cctcaagcgt acgacactga attccaagct gagaaagttg ggaattcgcc gttctgactt
     8101 catgtagcca tgtatggcga aatatcgtca gtgattttcg tcacccctcc tcatgatctg
     8161 gctcaccaac ggctaagcgt tcgctaatcc ttttagtgtc cgactcatag ctatctatcc
     8221 ttcttgtaaa cgctttggtt ttccgaatgc cttagtgata ggtgtcacgc gaaaggtacc
     8281 aatgacgcta aggatcacgg tgaacgagag tccagaaagc acgacgatga aactagaagg
     8341 caaacttatc ggaccttggg tcgccgaact cgatcgggca tggcgatcgc tactggtttc
     8401 cttgagcagc aaaaagcttc gcgtggattt atgcggaatc gtccgcatcg atcccaacgg
     8461 caggcaaatc cttgccgaaa ttcacagaca aaccggcgcc gagttcgttg cagatacgcc
     8521 gataaccaag tatttcgccg aacaggcgcg gctccagggt cagaaagact cgagcgaagg
     8581 agagtgacat gcgaggacca tacggtttcg agatgagcga gaattgccag accagcaaac
     8641 tcaagaagtg cggcttcttc tgcgacctca cgcctaaggc agtgaaggac ttcgattcaa
     8701 ttaagtcgac atcggcattt ccagacggtg ccgtgctttt catggaaaag caggatcccc
     8761 gaggagtgtt tgtactctgc cagggggaag ttaagcttac gatcagctcc agcgaaggaa
     8821 aaacgctcat cctgcgtatc gcgaaagcgg gcgagattct cggcttgatg tctgtcctcg
     8881 ccggcaagcc atacgaagtc acagcggaga ctcttcgtcc gtcccagatt gcctttatcc
     8941 gccgtgaaga ttttctgcgt ttcgtcgctc aacacccaga agtcagtgcg ggtatcgtca
     9001 agcagctgag cggcaactac cacaccgctt gcgaacaatt gcgcaccgtc ggcctctcta
     9061 cgtcggcgcg aggcagactc gcgaagttgc tgctggattg ggccgcagga gccgaacaaa
     9121 ccaagctcgg gaccaggatt cagatccctc tgacgcacga agaaattgcg gaatgcgttg
     9181 gcagttcgcg ggagaccgtc acacgcactt tcagtggatt caaaaaccag aacctcgtct
     9241 caatcaaagg ttcctctttg ctgattatca accgcgccgg actcgagagt tttgtcgcta
     9301 cctaaatttg agtgcgaatt ggtatccgtg agggcgtacg gcgcaggctg cggtttgagc
     9361 ctgcgttgcc gcagaaattg cgatcggctg ttcgggacta gtctagaata ccatcatgcg
     9421 cagagtcgca agggactggc tgttcatcgg taggatttgt gcggttttct tggtgctgat
     9481 tgcgctgctc gtatcgacac attctttgcc tgcttctcct ttgcaagagc atcgtctgca
     9541 gcgttaccac acccacacag gcgaaaggct ggacatcgtc taccgtcgcg ggggcacata
     9601 tgttagcgac gcgatcgcca agctcgacta cttcctttgc gatcaccgga ccggcgatgt
     9661 tcgacatttc gatcctaaaa tcttcgatgc tctagaagac ctcacgattg cggttggcca
     9721 tcctgatctg accgtgaccg gccccggtat ttttgaccca cctgaagggt tagtttccgg
     9781 caaaatccat gcctgaaaaa ggaggatttg tcgcgaagca gaagcgattc aacgtcgaac
     9841 agattgtggc ggtgttaaag caagcaggag cgggagtacc gctcgcagag ttgatccggc
     9901 gggttggaat ttccaaacag accttctatc gaatgggagc gagtttccca gccaggcgat
     9961 ggatctgtgg gcctatcaga acggagtgcg aatggcgttt tcccgtccag gaaaccgact
    10021 gatagtgcct ttgtggaatc gttcaacgga acgtttcggg cagaatgtct gaacgcgcat
    10081 tggttcatat cattgaccga agcccagcag atcgtagaga cttggcgctg ggaatacaat
    10141 gagagtcgcc ctcacagggc tttcggggag aagacaccca aagaatt
//
LOCUS       DATNDT010000363         8163 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3825117, whole genome shotgun sequence.
ACCESSION   DATNDT010000363 DATNDT010000000
VERSION     DATNDT010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8163
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3825117"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VN788_11610"
     CDS             <1..723
                     /locus_tag="VN788_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014241454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short-chain dehydrogenase/reductase"
                     /protein_id="HXU21219.1"
                     /translation="GASKGIGRATATTLAEEGCNLVLVSRDLAALNKVAEAISLRFSG
                     SVRTFAADLSKQDAIERLATEFAELDILVNNAGSIPPGGVLAIDNAAWRAAWDLKVFG
                     YISMCRALYPSLKKRKGVIVNIIGAAGERLDPGYITGSTGNAALMAFTKSLAKGAAKD
                     AMRVVGINPGPVSTDRLQTMMRGRAKAQFGDESRWEEFLSSMSFGRAAAPEEIASAVA
                     FLASPRSGYTSGTILTIDGAAS"
     gene            814..1725
                     /locus_tag="VN788_11615"
     CDS             814..1725
                     /locus_tag="VN788_11615"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008476391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HXU21220.1"
                     /translation="MEKLAPSFSTDLGAAYKTDCFNLFSALKEGSIDAVFADPPFNLG
                     KDYGNGSDRDDLASEKYLEWCSRWIAECVRVAKPGGAVFIYSLPQWAYHLAADLETHG
                     MLFRHWIAVSMKGTFPRGRKLYPAHYALLYFTKSIPKTFNRVRLPIPQCRHCRKDIKD
                     YGGHRKYLNPLGLSLTDFWDDTAPARHQKFKSRWHVNELKPIISGRCIDISTNPGEII
                     LDPFAGGGSSFEAAQKANRLWVGTEIVDCEPIRERFERNLPGVERSMPPAELSRAFTN
                     PDERLRILVPSCQESSKRNITTVHASV"
     gene            1749..2219
                     /locus_tag="VN788_11620"
     CDS             1749..2219
                     /locus_tag="VN788_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BglII/BstYI family type II restriction
                     endonuclease"
                     /protein_id="HXU21221.1"
                     /translation="MVEIKAIISEFTLRVREQVDANGGAAVRKLIDERFQAKGGWSNR
                     AAGDIDWTKCHEINGTKLCIGVEVQVSARSDLLVVDMIHLTAAFRDGRIDIGVLIVPS
                     DRLSRFLTDRGPCITDAKKHAKAARLEDSPLILLAIEHDGPGEALAKQAKRSSK"
     gene            2642..4579
                     /gene="thrS"
                     /locus_tag="VN788_11625"
     CDS             2642..4579
                     /gene="thrS"
                     /locus_tag="VN788_11625"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HXU21222.1"
                     /translation="MDNIQITLPDGATREVPQGTTAAQIAGQISPRLAKEALVARVNG
                     DLIDLSRPLEHDTKLSILTAKDPDAVQVFRHSAAHLLAAAVLELYPDVKLGVGPPIDT
                     GFFYEFVREQPFTPEDLAKIEGKMHELAAKDVPNERKLIPKPEALDLYRKSKQEFKCE
                     LVEEKAVEPMVSFYTTGKFIDFCRGPHIPSTGRIKAFKLMNVAGAYWKGDEKNPQMQR
                     IYGACFVEQKELDEYLHKLEEAKRRDHRKLGQELDLFSIQDEAGPGLIFWHPKGAIIR
                     TQMEDWLRAELTRRGYDLVYTPHIMRLDLWKTSGHANFYRENMFGAVEVEKADYQLKP
                     MNCPGHILIFKSHLRSYRELPVRLAELGTVYRYERSGVLHGLLRVRGFTQDDAHIFCM
                     PEQIEKEIQDCIEFAWEVLRVFGFDRYEVDLSGGDASHPETYVGTPEDWKRAEDALAN
                     TLKRMNVPYKYIPGEAAFYGPKIDVKLVDAIGRPWQLTTVQFDFNLPRRFELEYVGED
                     GARHQPLMVHRALFGSIERFFGVLIEHYAGAFPAWLAPVQAAVLPVSGKFEEYAKKVT
                     EQIKDAGFRVHLDDRNEKLQAKIRDAQLQKIPYMLILGGKEAEAGTVSVRHRGKGDLG
                     PRPLDQFIADLRQEVGSRAVQ"
     gene            4612..5244
                     /locus_tag="VN788_11630"
     CDS             4612..5244
                     /locus_tag="VN788_11630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21223.1"
                     /translation="MNVIASEAQGLLFSFAILCLGIAPSFSAAPQAQPDSGWMGKSFA
                     AALDEFFPIQRGEGDFIAVRAHRDGTNDLPEFSIALEDTQNPHAIHATLREAQGSSLY
                     QQLESLHAKEPSKSYAELKPDLKVQTRNLTADQCPAIAAQLKAFNNIQFVRPRDEDPV
                     AEHPILYQFHESVGGGDSEVTEFVETRAFPKWANETHHALDACAASAPGK"
     gene            5247..6422
                     /locus_tag="VN788_11635"
     CDS             5247..6422
                     /locus_tag="VN788_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020553743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HXU21224.1"
                     /translation="MDAKSAARERFKSAREGLIGLSRRIHARPELGFEEKYASSWLCE
                     SLAEAGFTVEKGICDLPTAFRARVGSGPLHVGICAEYDCLPGIGHACGHNIIAASAVG
                     AALAAAKVADEVGLTVSVIGTPAEEVGNASGKILMLERGAFDGLHAAMMVHPAPFDML
                     HAKIIAASMFEVHCTGKESHASAFPELGVNAADALTIAQTALGLLRQHIRSTDRIHGI
                     VTNGGAAPNVVPAHTSAKYIVRSETLEQLAELRPKVYRCFEAGGLATGAKIEITGGDK
                     PYAEMLHDASMAAFYRKNSEALGRPFPDLGEWETRPTGSTDMGNVSLAVPSIHPMIGI
                     NSLPAVNHQPEFTAHCITADADKALEDGALAMAWTCIDLAADREARERLMAKSAQNR"
     gene            6548..7129
                     /gene="infC"
                     /locus_tag="VN788_11640"
     CDS             6548..7129
                     /gene="infC"
                     /locus_tag="VN788_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008955136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HXU21225.1"
                     /translation="MAERSGSPGLRVNERIRAREVRVVDDAGGQLGVMTLFDAMKAAR
                     EAGLDLVEISPSAVPPVCKLVDYGKFLYEQSKKAHEAKKHQKSSHIKEVKFRPATAEH
                     DYQVRKNQIIRFLGEGHKVKAMIFHRGREMAHQSVGRAKMERLLKDINDHALVEFGPR
                     MEANILLALLAPKKGAPAPQPSRPAQSTAGGQA"
     gene            7148..7366
                     /gene="rpmI"
                     /locus_tag="VN788_11645"
     CDS             7148..7366
                     /gene="rpmI"
                     /locus_tag="VN788_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HXU21226.1"
                     /translation="MKKNKKTKLKTHRGAAKRFKITSTGKVLRWHSGKRHLLGTKKPR
                     RQRKLLNKVEVNPADVHNVRLALPYGIQ"
     gene            7594..7980
                     /gene="rplT"
                     /locus_tag="VN788_11650"
     CDS             7594..7980
                     /gene="rplT"
                     /locus_tag="VN788_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009672035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HXU21227.1"
                     /translation="MARVKRGTKRRARRKKLLKHAKGFFLTKGKLFRSAKEAVNRSLR
                     YTYRDRRTRKRDYRTLWIQRIGAAARANGTTYSQLIHGLKAGGIDLDRKILADMAVRD
                     AEGFSALVASAKQHMKAAPAPPQSAA"
BASE COUNT         1865 a         2273 c         2398 g         1627 t
ORIGIN      
        1 ggcgcttcca aaggcattgg ccgcgcgaca gccactactt tggccgagga aggctgcaat
       61 ctcgttctgg tctcacgcga tctggcggca ctgaacaaag tggccgaggc gatcagtctc
      121 cgattttcgg gttcggtgcg cactttcgct gccgaccttt ccaagcagga tgcgatcgaa
      181 cgcctggcga cggaatttgc cgaactggat atccttgtca acaatgctgg ctcgattccg
      241 ccgggtggcg tgctcgccat cgacaacgcc gcttggcggg cggcatggga tttgaaagtt
      301 ttcggataca taagtatgtg ccgcgcgttg tatccgagct taaaaaaacg gaaaggcgtg
      361 atcgtcaata tcatcggcgc cgctggggaa cgtctcgatc ccggctatat caccggaagt
      421 acggggaacg cggcactcat ggcgttcacg aagtcgcttg caaaaggagc cgcaaaggat
      481 gcgatgcgcg ttgtcggcat caatcccggc cccgtgtcca ctgatcgttt gcaaacgatg
      541 atgcgaggcc gcgccaaagc tcaatttggc gatgagtcac ggtgggaaga attcttaagc
      601 agcatgtcat ttggtcgcgc cgcggcaccg gaagaaattg ccagcgctgt agccttcctc
      661 gcatcgccgc gctccggcta tacctcgggc accatcctga ccatcgacgg cgccgctagc
      721 tgattgccgg tacgctcccc acgcaatgcc ggtgccaacg aaccggcgtc gaccgaaaag
      781 tggcatcaag aatgctatac ttcctggaaa cttatggaga agcttgctcc ctcattcagc
      841 acggacctcg gtgcggcgta taaaacggac tgcttcaacc tatttagcgc gctgaaagag
      901 ggatcgatag acgccgtttt cgccgatccc ccgttcaatc tgggaaaaga ttacgggaat
      961 ggttccgaca gggatgacct cgcatccgag aagtacctgg aatggtgctc acggtggatt
     1021 gcggaatgcg tcagagtcgc aaagccaggc ggagctgtct tcatctatag tttgccccaa
     1081 tgggcatacc atctggctgc cgaccttgaa actcacggaa tgctgtttcg gcactggatc
     1141 gcagtgtcga tgaaggggac gtttcctagg ggcaggaagc tttatccggc tcactacgct
     1201 ttactgtatt ttaccaaaag catacccaag acctttaatc gggtgcggct cccaattcct
     1261 caatgccggc actgccgaaa ggacatcaag gactatgggg ggcatcgcaa atacctgaac
     1321 cctttgggcc tgagcttaac tgacttttgg gacgacacag cgccagcgcg ccaccagaaa
     1381 ttcaaatccc gatggcacgt aaacgagctg aagccgatta tttctggtcg ctgcattgac
     1441 atttccacaa atcctggcga aatcattctg gatccttttg cggggggagg gtccagcttc
     1501 gaggcagcgc agaaggccaa ccgtctgtgg gtcggtaccg aaatagtcga ctgtgaacca
     1561 atacgggaac gcttcgagcg caacctgcct ggcgtcgaac gttcaatgcc gccagcggag
     1621 ctgtctcggg cctttacaaa tccagacgag cggctcagaa tcctcgttcc ttcatgccaa
     1681 gaatcgtcga agagaaatat tacgacggtg cacgcgagcg tatagagagg cttggacttg
     1741 cgcctctcat ggtggaaata aaagcgatca tttccgaatt cacgcttcgc gttcgcgaac
     1801 aagttgatgc gaacggcggc gcggctgtgc gcaagctgat cgacgagcgc tttcaggcca
     1861 aaggaggatg gtcaaataga gccgctggcg atattgactg gacgaaatgt catgagatca
     1921 acggtaccaa gctctgcatt ggtgtcgagg ttcaagtatc agccagatcc gatctgctcg
     1981 tagtggacat gatccacctc acggctgcct tccgagatgg aagaatcgat atcggcgtgc
     2041 taattgtgcc cagcgataga ttaagtcgct tcctcactga tcgcggtccg tgtatcaccg
     2101 atgcgaaaaa acacgccaaa gccgcacgtc tcgaagactc cccgctgatt ctgttagcga
     2161 tcgaacacga tgggcctggt gaagcgctgg ccaagcaggc caaacgttct tccaaatagt
     2221 ctcgcttgaa atcccttcga ctctcaggta ctcagcaatt ctttacacta acaagcggat
     2281 gaaatcgctt gacggatcat gttacggtta tcgccggtcg cgagtagcgc tcgcgcccag
     2341 gatttttgag ccaagtgagg ggaaaaatgg aagcgaagat ggaacggtgc agggaacacg
     2401 agttcggtac ggggcgactc ttgttgccgt cgcggcgcgg gcgacccgtc tgtgagggtg
     2461 gcttttttgc gccgagtatc aagggaaaag ccggattcta cctaactgta ctggcgagta
     2521 gaaatggtcg taactacatg aaaataaaga gtcggtgccg ctgctacccg tcacaaaaac
     2581 gggaggggcg attccgcagt ctgtggctac tttcaaacgt ggttctagct caaaattaga
     2641 tatggataac attcaaatca cgctgccgga tggagcgacg cgcgaggtgc cgcaaggcac
     2701 caccgccgcg cagattgccg gccaaatctc gccgcgcctg gcgaaggaag ctctcgtcgc
     2761 gcgcgtcaac ggcgacctga tcgatctctc gcggccgctg gaacatgaca cgaaattgtc
     2821 gatcctcacc gccaaggacc cggacgcggt tcaggtattc cgccactcgg ctgctcatct
     2881 gctcgctgcg gccgttctcg aactttaccc ggatgtaaaa ctcggcgtcg gcccgccgat
     2941 tgacaccgga ttcttttacg aatttgtgcg cgagcagccg ttcactcccg aggacctcgc
     3001 aaaaatcgag ggcaagatgc acgagttggc cgcgaaagat gttcccaacg agcgcaaatt
     3061 gattccgaag cccgaggcgc tcgacctcta tcgcaaatcg aagcaggaat ttaagtgcga
     3121 actggtggaa gaaaaagccg tcgagccgat ggtttccttt tacaccaccg gaaaatttat
     3181 cgacttctgc cgcggtccgc acatcccttc caccgggcgc ataaaagctt ttaaattgat
     3241 gaatgtcgcc ggcgcctact ggaaaggcga cgaaaaaaat ccgcagatgc agcggattta
     3301 cggcgcgtgt ttcgtcgagc agaaggaact cgacgaatat ctgcacaaac tcgaagaggc
     3361 caagcggcgc gaccatcgca aactcggcca ggaactcgat ctcttcagca ttcaggatga
     3421 agccggcccc ggcctgattt tctggcatcc caagggcgcg atcatccgca cgcaaatgga
     3481 agactggctg cgcgcggaac tgacgcgacg cggctatgat ctggtctaca cgccgcacat
     3541 tatgcggctc gatctttgga aaaccagcgg gcacgcgaat ttctatcgcg aaaatatgtt
     3601 cggcgccgtg gaagtggaaa aagcagatta ccaactcaag cccatgaact gcccgggcca
     3661 cattttaatt ttcaaatcgc acttgcggag ttatcgcgaa cttcccgtgc ggctcgccga
     3721 gcttggcacc gtctatcgct acgaacgctc gggcgttttg cacggcttgc tgagagtccg
     3781 cggcttcact caggacgacg cgcacatctt ctgcatgccg gagcagatcg aaaaggaaat
     3841 tcaagattgc atcgagttcg cctgggaggt gttgcgcgtt ttcggtttcg ataggtacga
     3901 agtggatctt tcaggcggcg acgcgtcgca tcctgaaacc tacgtcggca ccccagaaga
     3961 ttggaaacgc gccgaagatg cgctcgccaa cactttgaaa cgcatgaatg tgccgtacaa
     4021 atatattccg ggcgaggcgg cattctacgg tccgaaaatc gatgtgaagc ttgttgacgc
     4081 gattggccgt ccatggcagc tcaccaccgt gcaattcgat ttcaacctgc cgcgccgctt
     4141 cgaactcgag tatgtcggcg aagacggagc ccgccaccaa cctttaatgg tccatcgcgc
     4201 gctgttcggc tcgatcgaaa gatttttcgg cgtgctgatc gagcattatg cgggagcatt
     4261 cccagcatgg ctcgccccag tgcaagcggc ggtattgcca gtcagcggga aattcgagga
     4321 atacgcaaaa aaagttacgg agcagatcaa ggatgccggt ttccgcgtcc atctggacga
     4381 tcgcaacgaa aaactacagg ccaaaatccg cgacgcacag ctgcagaaaa ttccttacat
     4441 gctgatcctc ggcggaaaag aagcggaagc cggcacggtg tcggtgaggc atcgcggcaa
     4501 gggggacttg ggtccgcggc cgctcgatca gttcatcgcc gatttgcggc aggaggtggg
     4561 ctcgcgcgcg gtgcagtagc gcagaataat ttgtatgagc gataaagcta aatgaatgtc
     4621 atcgccagcg aagcgcaggg tctgcttttc tctttcgcga ttttatgcct cgggatcgcg
     4681 ccgagttttt ccgccgctcc tcaggcgcag cccgattccg gctggatggg caagtctttc
     4741 gcggccgcgt tggatgaatt ctttccaatt cagcgtggcg aaggagactt catcgcggtt
     4801 cgcgcgcatc gggacggcac gaatgattta ccggaattct ccattgcgct cgaggatacg
     4861 cagaacccgc acgcgattca cgccactctg cgcgaggcgc aaggttcttc gctatatcaa
     4921 cagctggagt cactgcatgc caaggagccg tcgaaatcct acgcggagct gaagcctgac
     4981 ctgaaagttc aaactcggaa tctcactgcc gatcagtgtc cagccatcgc ggcgcaactc
     5041 aaagccttca acaacataca attcgtgcgg ccccgcgacg aggatcccgt cgccgagcat
     5101 cctatccttt atcaattcca cgaatcggtg ggcggaggcg attccgaagt caccgaattt
     5161 gttgagacgc gcgcgtttcc gaaatgggcc aatgaaacgc atcatgcgct cgacgcatgc
     5221 gccgcttcgg cgccgggaaa atgacaatgg acgcaaaatc cgcagcgcga gaacgtttca
     5281 aatccgcccg cgaaggactg attgggctga gccgccgcat ccatgcccgt cccgagctgg
     5341 gcttcgaaga aaagtacgct tcttcctggc tatgtgagtc gctcgccgaa gccggtttca
     5401 cggtggaaaa gggtatttgt gatcttccta cggcgttccg ggcgcgcgtc ggatcgggtc
     5461 ctttgcatgt cgggatttgc gccgaatacg actgtttgcc gggcatcggt cacgcatgcg
     5521 gccacaacat tatcgccgca tcggccgtcg gcgcggcgct ggctgcagcc aaagtcgccg
     5581 atgaggtagg cctcaccgtg agtgtgatcg gcacgccggc tgaagaagtg ggcaacgcga
     5641 gcgggaaaat tctgatgctt gaacgcggcg cgtttgatgg ccttcacgcc gccatgatgg
     5701 tgcaccccgc gccatttgac atgctccacg caaagatcat tgcggcttcc atgtttgagg
     5761 tgcattgcac ggggaaggag tcccacgcat cggcctttcc ggaattgggc gtgaacgccg
     5821 cggatgcact aacgatcgcg caaaccgcgc tgggcctatt gcggcagcat atccggtcca
     5881 ccgatcgcat ccacggcatt gtgaccaatg gcggcgcggc tccgaatgtg gttcccgcgc
     5941 atacttcggc caagtacatc gttcgctcgg aaactctgga gcaacttgcg gaattgcgac
     6001 ccaaagtata ccgatgcttt gaagccgggg gcctcgccac gggcgcgaaa atcgagatca
     6061 ccggagggga caaaccttac gccgagatgc tgcacgacgc atccatggcg gcgttttacc
     6121 gcaagaattc cgaggcgctg gggcgtccgt tccccgatct tggcgagtgg gaaactcggc
     6181 cgactgggtc caccgacatg ggcaacgtct cgctggcagt gccgtcgatt catcccatga
     6241 tcggcatcaa ttccttgccg gctgtcaacc accagccaga attcacggct cactgcatca
     6301 ccgcggacgc ggacaaggcg ctcgaggacg gcgccctggc gatggcgtgg acttgcattg
     6361 acttagcggc tgaccgcgaa gcgcgggaaa gattgatggc aaaatcggct caaaatcggt
     6421 gacgaacctt attgagaacg atgcgcggag gcatgctata ctttttgccc tgtcactctg
     6481 tcggggtgtt cagagcgaca ggtaaatcgg gcggcaggtt aatcgggtcc gtccgggagg
     6541 tgggtttatc gccgagcgta gtggaagtcc cggccttcgg gtcaatgagc gcattcgcgc
     6601 tcgtgaagta cgcgtagtgg atgatgcggg cggccaactg ggtgtgatga cgctgttcga
     6661 cgcgatgaaa gcagcgcgcg aggcgggtct cgacctggtg gaaatttcgc ccagcgccgt
     6721 gccccctgtc tgcaagctcg tggattacgg aaagtttctc tacgagcaga gcaagaaggc
     6781 gcacgaagcc aagaagcacc aaaaaagttc gcacatcaag gaagtaaagt ttcggcccgc
     6841 gactgcggag cacgattatc aggtgcgcaa gaaccagatc atccggtttc tgggcgaagg
     6901 tcacaaagta aaggcgatga ttttccaccg cgggcgcgag atggctcacc aaagtgtggg
     6961 ccgcgccaag atggaacggt tgctcaaaga catcaacgat cacgctctgg tggaattcgg
     7021 gccacggatg gaagcgaata tcctgctggc gctgttggcg cccaaaaagg gcgcacccgc
     7081 gccgcagccg tcgagaccgg cgcaatccac cgcgggcggc caagcctaaa aacgcgaaga
     7141 gggaattgtg aagaagaaca agaaaacgaa attgaagacg caccgaggcg cggccaagcg
     7201 ctttaagatt acgtcgactg ggaaagtgtt gcggtggcat tcgggcaagc gccatctact
     7261 tggcacgaag aagccgcggc ggcagcgaaa gctgctgaat aaggtcgagg tgaaccccgc
     7321 cgacgtgcat aacgtgcgtc tggcgcttcc ctacggaatc cagtagcaga ggttctaggt
     7381 ttttcgagcc aggcagagcg ttacgggtgc ctgccggccg gttgccctga atcactgagg
     7441 gcgcatttgt agtccacgag acaaacccgt tttgcagccc gtgtgaaatg tcgcgggcag
     7501 cgaagccgcg tttgtctccc cgtttgtttt tacttttacc aaatccgaaa tcattgagag
     7561 cggccgcgag gcgcgcacga ggagaagagg accatggctc gagtaaaacg aggtacgaaa
     7621 cgccgcgcgc ggcggaagaa actgctgaag cacgcgaagg gatttttcct cacgaaggga
     7681 aaactgttcc ggtcggcaaa ggaggcggtg aaccgctcgc tgcggtacac ctatcgcgac
     7741 cggcgcacgc gcaagcgcga ttaccgcaca ttgtggattc agcgcatcgg cgccgccgca
     7801 cgagcgaacg gaacgaccta cagccagtta attcacggcc tgaaggcggg cgggattgac
     7861 ctcgaccgga agattctggc cgacatggcg gtgcgcgacg ccgaaggatt ctcggcgcta
     7921 gtcgcgtctg cgaagcagca catgaaggcg gcgcccgcgc cgccgcagtc tgcggcctaa
     7981 aagatgcaca ggcaagagtg cccgtgccac gtgcttgcgc cacgcaaacc atgcctgatt
     8041 cacccattat tgagaaaacc ctcaacgagt taggcgacac aactcctgag atggttgcaa
     8101 ttcgcttcgc cgaattgtgt tcgtccttcg atgtggaggc ggcgcgccag tctgcggacc
     8161 cgg
//
LOCUS       DATNDT010000364         3352 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2661398, whole genome shotgun sequence.
ACCESSION   DATNDT010000364 DATNDT010000000
VERSION     DATNDT010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2661398"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..302
                     /locus_tag="VN788_11655"
     CDS             <1..302
                     /locus_tag="VN788_11655"
                     /inference="COORDINATES: protein motif:HMM:NF013845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HXU21228.1"
                     /translation="VNAMIAAGWTDEVRRLVEMGVPANAKPFQFIGYSDLRDQISNHR
                     PPAEVVEKIQQATRRFAKRQITWFRKEPDVHWLSGFGDAPEIIAAALKFTRGLAV"
     gene            375..2021
                     /locus_tag="VN788_11660"
     CDS             375..2021
                     /locus_tag="VN788_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S10"
                     /protein_id="HXU21229.1"
                     /translation="MIPRTEQHSLRSLIFWIVATISIPAFRPAIVHAQATSETSVSKT
                     ETAQTPEQFKPEEQASKGSVTVDGNMINYDAHAGTIIVHPKGWDDVPQNNTDKDPKNQ
                     PAEASMFYVAYFKSDAKDTQRPVTFLYNGGPGSSTMWLHIGAFGPRRVVTSDDSHTPA
                     APYSIVNNDYSLLDVSDLVFVDAPGTGFSRISGKDREKAFYGVDQDAWAFADFIVQFL
                     TKYGRWNSPKYLFGESYGTTRSAALSNVLETERDLDLNGVILLSQILSFDLSADSPQF
                     NPSIDLPYELALPTYAATAWYHHKLPDAHPDLAPLLTEVEHFAMTEYAEALEAGSSLA
                     PEQRQAVVAKLHAYTGLPVEYIEKADLRINGGEFEKTLEGDMDLTTGRLDTRFSGPTF
                     DPLSKEAGYDPQAAAISSAYASAFNDYVRKDLKFNVPREYKPEIELWKSWDHLHQPPG
                     ATQPQKQATNVMPDLANAMKYNPNLKVLLNAGYFDLATPFYEGIYEMHHLPIPPKLQN
                     NIEFKFYESGHMVYAHAAALKTLHDNVAAFILKTETPKTK"
     gene            2127..>3352
                     /gene="lepA"
                     /locus_tag="VN788_11665"
     CDS             2127..>3352
                     /gene="lepA"
                     /locus_tag="VN788_11665"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HXU21230.1"
                     /translation="MDPRFIRNFCIIAHIDHGKSTLADRLLELTGAVTQREMTAQVLD
                     SMDLERERGITIKAKSIRLHYRAKDGNEYRLNLIDTPGHVDFSYEVSRALSACEGALL
                     VVDASQGVEAQTVANAFLASRHNLAIIPVINKIDLPAAQPDYAKEQIENVLAIPADDA
                     LLISAKSGLGVEDVLEAIVKHVPPPKGSADAPLRALIFDSWFDSYRGAVVLVRVMEGR
                     MAPRMKVRLSASDEVYEVENLGVLTPKPVALEELAAGDVGFVIANIKRVADARVGDTV
                     VEADRPAEPLPGFEAIKPMVFAGLFPVNASDYEMLRDALGKLQLNDAAFFYEPENSVA
                     LGFGFRCGFLGLLHMEIAQERLEREFNIDLITTAPSVQYRITTTDGEVKEVDNPTKFP
                     PPTSIQKIEEPILTAM"
BASE COUNT          738 a         1011 c          919 g          684 t
ORIGIN      
        1 gggtaaatgc catgatcgcc gccggatgga cagatgaagt tcgcagactt gtcgaaatgg
       61 gcgtgcccgc gaatgcgaag ccgtttcagt ttatcggcta ttcggatttg cgcgatcaga
      121 tttccaatca ccggccgccc gctgaggtcg tcgagaaaat tcagcaagct actcgccgtt
      181 ttgccaaacg ccaaatcacc tggttccgca aggaaccgga cgtgcattgg ctctcgggat
      241 ttggcgatgc cccggagata atcgccgccg cgctgaaatt cacgcgaggt ttggccgtct
      301 gaaaatttgg cggcgcaccg cacgcgtcca gtatacttct gcttcatctg ccaatcatga
      361 aaggaagccg tacgatgatt ccgcgaacgg agcaacactc gctgcgaagc ctcatttttt
      421 ggatcgtcgc cacaatctcg atcccggcgt tccgtcctgc gatcgttcat gcgcaagcaa
      481 caagcgagac gtcggtgagc aagacggaaa cggcgcaaac acccgaacaa ttcaagcccg
      541 aggaacaagc cagcaaaggc tccgtcaccg tggacggaaa tatgatcaat tatgacgccc
      601 atgccggcac gatcatcgtc caccccaagg gttgggacga cgtcccgcag aataataccg
      661 acaaggatcc caagaatcag cccgccgaag ccagcatgtt ttacgtcgcc tacttcaaat
      721 ccgatgccaa ggatacccag cgcccggtga cttttctgta caacggcggc cctggatcgt
      781 cgacgatgtg gctgcacatt ggagcgttcg gtcccaggcg cgtcgtcacc tccgacgatt
      841 cgcacacgcc cgcggcgccc tactcgatcg tcaataacga ttacagcctg ctggatgtaa
      901 gcgacctcgt ttttgtggac gcgcccggta ccggctttag ccgcatcagt ggcaaggacc
      961 gcgaaaaagc tttttacggc gtggatcagg acgcgtgggc gtttgccgat ttcatcgttc
     1021 agttcctcac gaaatacggg cgctggaact cgccaaagta tttgtttggt gaaagctacg
     1081 gcaccacgcg ctctgccgct ctttccaacg tactcgaaac cgaacgcgac ctcgatctca
     1141 atggtgtaat tctgctatcg caaatcttga gcttcgacct cagcgcggat agcccgcagt
     1201 tcaatcccag tatcgacttg ccctatgaac tcgcgctacc cacttatgcc gccaccgcgt
     1261 ggtatcacca caagttgccg gatgcgcatc cggacttggc gccgctgctc acggaggtcg
     1321 agcacttcgc gatgacggaa tacgccgagg cgcttgaggc cggctcgtcg ctcgctcccg
     1381 agcagcggca agccgtcgtc gcgaagctgc atgcgtacac aggcctcccc gtcgaataca
     1441 tcgagaaggc cgacctgcgc atcaacggcg gcgaattcga gaagacgctc gaaggcgata
     1501 tggatttgac caccggacgc ctcgatactc gcttttccgg acccacgttt gatccgctca
     1561 gcaaggaggc cggttacgat ccccaggcgg ccgcgatcag ttccgcttac gcttctgcgt
     1621 tcaatgatta cgtgcgcaaa gacctgaagt tcaacgtgcc ccgggaatat aagccggaaa
     1681 tcgaactctg gaaatcgtgg gatcatctcc atcagccccc cggcgccact cagccgcaga
     1741 agcaggcgac aaacgtgatg cccgatttgg ccaacgcgat gaaatacaac ccaaatctca
     1801 aagtcctgtt gaacgccggc tatttcgatc tcgccacgcc attctacgaa ggcatctacg
     1861 aaatgcatca cttgccgatt cccccgaaat tacaaaacaa cattgaattc aagttttatg
     1921 aatcgggcca catggtgtac gcgcacgcgg ctgcgctgaa aacgctgcac gacaacgtgg
     1981 cggcttttat cctcaagact gaaactccca agaccaagtg agcggcgcgc tccgaactgt
     2041 ggcacactcg attgtttgca ggacgcggcc ggtgttctcc gctcttcgct tgcggccacc
     2101 cggacccgcg acccgcacgg gaacccatgg atcctcgttt catccggaat ttctgcatca
     2161 tcgcgcacat cgaccacggt aaatccacgc tggccgatcg cctgctggaa ctcaccggcg
     2221 cggtcaccca gcgcgaaatg accgcgcagg tcctcgattc gatggatctc gaacgcgagc
     2281 gcggcatcac gatcaaggcc aaaagcatcc gtttgcatta ccgcgcgaag gacggcaacg
     2341 agtaccgcct gaatctgatc gatactccgg ggcacgtcga tttttcctac gaagtttccc
     2401 gcgccctttc cgcgtgcgag ggcgctttgc tcgtcgtcga cgcaagccag ggagtggagg
     2461 cgcaaacggt tgcgaatgcg tttttggcct cgcggcacaa tctggcgatc attcccgtga
     2521 ttaacaaaat cgacctgccc gccgcgcagc ccgattatgc caaggaacaa attgaaaatg
     2581 tgctcgccat tccggcggac gacgcgctgc tgatcagcgc caagagcggc ctcggcgtgg
     2641 aagatgtcct cgaagccatc gtaaaacacg tgccgcctcc gaaaggctcg gccgacgcgc
     2701 cgctgcgcgc tttgattttc gattcgtggt tcgattccta tcgcggagcc gtagtgctgg
     2761 tgcgcgtgat ggaaggccgc atggcgcctc gtatgaaggt tcggctttcg gcgagcgacg
     2821 aagtttacga agtcgagaat ctgggagtcc ttactcctaa gcccgtcgcg cttgaggaat
     2881 tggcggcggg agatgtgggt ttcgtcatcg caaatatcaa gcgagtggcc gacgcgcgtg
     2941 tgggcgatac ggtggtggaa gcggaccggc ccgccgagcc gcttccagga tttgaagcca
     3001 ttaagcccat ggttttcgcc ggactttttc ctgtgaatgc cagcgattac gaaatgctgc
     3061 gcgacgctct cggcaagctg caattgaacg acgctgcgtt tttttacgag ccggaaaatt
     3121 ccgtcgcgct cggcttcggc ttccgctgcg gctttcttgg cttgttgcac atggaaatcg
     3181 cgcaggagcg tttggagcgc gagttcaata tcgatctgat taccaccgcg cccagcgtgc
     3241 agtaccgcat caccaccacg gatggcgaag taaaagaagt cgataacccc acgaaatttc
     3301 cgccgcccac ttcgattcag aaaatcgagg agccgatttt gacggcgatg at
//
LOCUS       DATNDT010000365        13487 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3047480, whole genome shotgun sequence.
ACCESSION   DATNDT010000365 DATNDT010000000
VERSION     DATNDT010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13487
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3047480"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1686)
                     /locus_tag="VN788_11670"
     CDS             complement(<1..1686)
                     /locus_tag="VN788_11670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21231.1"
                     /translation="MKNRAFTLMGSIVCLAVMLGFPRATKAQGEAAEVARMYKACTDS
                     GGTAASNYNAWVAQNGCICPGSSVGSGQVTCSGGSNSVATSAPSPNLNLTPQQQVGMQ
                     IGMLGGYMVGQGLHQLLFGAPPSPPDTAAQQRQLAARQLNNSGIYLFRQKNYAGAINE
                     FQKALAITPGDGNILRNLALAKQKLKDVAMAGQTSNALGQLLESAPASAGTLNSALTG
                     TGGANGPALNLVDLDSDPGAVDLRGTTKTHLNPEQAQTLQSLDNVLGNGSDAEVKRQL
                     DDFANNDLIQDSGSNNVEPAPNAILETKSSQPDAETVKEIDQAIGQNQSADTQPPAAA
                     VEGNRAVAVSANSAQQSRSHLLDGIGNPDFDGTTTGNAPLGKADPNPDSDAVDLRRAS
                     TTVVDPARVDGTSPALQTMNPSDADKALMKHQWEMSIDSRYKNDPEVQQNIRDLWTAA
                     RSGNADSDEKLKGILSDQFNAAGETPQQRADFFANFNAIARDEGPTPKEWSTVSQLAN
                     EMDTASSSLRPAGADSAELPPYYGQLVKDIGKAESVTANASYMGAGRQTQDDCV"
     gene            complement(1900..2784)
                     /locus_tag="VN788_11675"
     CDS             complement(1900..2784)
                     /locus_tag="VN788_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009522381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase N-terminal
                     domain-containing protein"
                     /protein_id="HXU21232.1"
                     /translation="MPWQEKEESEDVTAAEFQAWVGKTETRTDQITSMPVAALSATLD
                     IDASAPRPGDMVPPLWHWMWFLPIHKQSELGPDGHAKTGDFLPPVPLPLRMWAGGRFE
                     FHHPLRVGQTYTRTSRILDVQQKEGRSGPLIFVIVRHEIGYAEGIALSEEQDIVYRDN
                     ATADQVGTAATQPAPSNAAWERVVQPDDVLLFRYSALTFNAHRIHYDRRYATEVGGYP
                     GLVVHGPLIATLLLDLIRRNLPNATVEHFAFRAVNPLFDTEPFVLCGKPESDGKTVSL
                     WARDTAGGLATTATATRV"
     gene            2860..4101
                     /locus_tag="VN788_11680"
     CDS             2860..4101
                     /locus_tag="VN788_11680"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU21233.1"
                     /translation="MHSSAQITAQKNSDSTIPIAVGWRMWIPVVAMMTCSWLSYVDRQ
                     ALAVISPMILRDTGLSAASYADAISVFSIVYMIANPLWGSLLDYVGLRAGMLVAVGIW
                     TIASASHAWVMGFLGFAMARGLLGFGEGATFPGGLRTAADSLPIDRQSRGMGISYSGA
                     SVGAIFAPLIITPIALRFGWRAAFIATGALGAGWLAIWWFVARPPLVRAVNRSKRKVT
                     WPNLLERRFWLLVVGMGLGGAALGPTLYLSPLYLNRVLGLTQGQLGRILWIPALCWEL
                     GYYFWGWIGDRFVRENPRPIRLYILLTILALPLSLITETTSWVAALAFFSWAMFIAVG
                     FIVISLHLSARAYPRDETGMVAGIGSGAWSAVVAILLVIYGRWFDRQLYAATFVSLSL
                     VPVLGTALWWWIGREPSRAAR"
     gene            complement(4098..5585)
                     /locus_tag="VN788_11685"
     CDS             complement(4098..5585)
                     /locus_tag="VN788_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HXU21234.1"
                     /translation="MKKHKMLVGGKWVDPASGEWFESVNPYTAKPWALIPRGTKDDID
                     QAVAAAKCAFYGDAWRKLTATARGALLSRLADLIAAEAAKLGEIETTDNGKLIAEMGG
                     QVKYVPQWFRYFGGLADKIEGRVIPIDKPGVFNFTREEPVGVVAAITPWNSPLLLATW
                     KLAPALAAGNTVVWKPSEFASVSALTFGELFERAGFPPGVVNIVTGYGNEVGEPLIAH
                     RDVAKVAFTGGDRTGEHVYQLAARGLKRVTLELGGKSANIVFDDADMEEALKGIVSGI
                     FAASGQTCIAGSRALIHRPIHDKFVEKLLAMAKTARMGNPLDPATQVGPITTRPQYEK
                     VLDYIRIAKEEGAVCRLGGTPAQRPECGVGWFVEPTIFTGVRPDMRIAQEEVFGPVLS
                     IIPFDTEEEAVKIANGTVYGLAAGVWTSSIRRALLMSEQLEAGTVWVNTYRAVSYMSP
                     FGGYKRSGIGRESGIDAIREYLQTKSVWIDIVGNAPNPFIMRMGD"
     gene            6009..6941
                     /locus_tag="VN788_11690"
                     /pseudo
     CDS             6009..6941
                     /locus_tag="VN788_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008969133.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            7065..7457
                     /locus_tag="VN788_11695"
     CDS             7065..7457
                     /locus_tag="VN788_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HXU21235.1"
                     /translation="MDQKTFERGKAIRSSVLGKEYVDNAFQTADDFNRPLQELVTEYC
                     WGAIWGREELPKKTRSMLNLAMISALNRPHELKSHIKGALVNGVSMVEIREVLLQVAI
                     YCGVPAAVDSFRIAREVFAEQKEKAPKA"
     gene            7476..8285
                     /locus_tag="VN788_11700"
     CDS             7476..8285
                     /locus_tag="VN788_11700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXU21236.1"
                     /translation="MAIEPFEVFAIRYAHLGGRHPGENFVLADPHEFASDLDYFVWVL
                     RRGDSSFVIDTGFGKEAAARRRREWLRSPVESLRLVGVNPDQVGDVIITHLHYDHAGN
                     LDKFPRARFHLQDRDMAYATGRCMCHQTLRAPFDLENMLEMVKHVYSDRVEFHDGDAE
                     LAAGLSLHRVGGHSAGLQIARVWTRRGWVVIASDATHLYANFEQRRPFPIVYNVAEML
                     EGFKMLYRLADSPDHIVPGHDPLVMKYYPAPKPELAGIVVRLDVAPIKSPA"
     gene            complement(8322..9719)
                     /locus_tag="VN788_11705"
     CDS             complement(8322..9719)
                     /locus_tag="VN788_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015321733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0051119 - sugar transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HXU21237.1"
                     /translation="MSATTVSTPPGSSGGFVYVAAAFAGLGGLLFGYDTGVISGAELF
                     LGKDFTLSSFALEVIVSGVLVGAAIGALGGGRLADLYGRRRLLIATAVIFAIGAIICA
                     AASSPAVLIIGRIIVGLGIGLSSGTVPVYISEVSPPAARGWTVSIFQLAITVGILLAY
                     VVDYAFAAIQGWRWMLGLAVVPAAVFALGMILLPESPRWLARKGHLEAARKVLGRIRT
                     AAGADAEMKDIEASIQHAEEHGRLSDLFHPSLRAALVVGIGLAILQQITGINTVIYYA
                     PLIIRSAGIPSASGAILATAGIGVVNVVMTIVSMWLIDRAGRRPLLLTGIAGMIITLF
                     ALGYAFHSPMHGGSLATVAVICMMLYVASFAISLGPIFWLLIAEIYPLPVRSSAEGLS
                     ATFNWGANLLVSLTFLTLLQLMGASRTFWLYGIFAIGAWFFSYYLVPETKGRTLEEIE
                     EFWRKRHVAPKTARL"
     gene            complement(9725..11440)
                     /locus_tag="VN788_11710"
     CDS             complement(9725..11440)
                     /locus_tag="VN788_11710"
                     /inference="COORDINATES: protein motif:HMM:NF013377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalase family glycosidase"
                     /protein_id="HXU21238.1"
                     /translation="MRVGGSRYFRALCLPLFAAILIAATLASHASTALPPPQTAASEN
                     SFQSLDDILHYISDDWTGLKRSLTECATYQDVKTKGKPTLYLPADVSISPALAQMQQR
                     CHLQIKHLPANLSEEDAGKIQPAGLLYLENPYVVPGGRFNEMYGWDSYFIIRGLLRDN
                     HRDLAKGMVENFFYEIQHYGGVLNANRTYYLGRSQPPFLSSMILAVYQADRGAGHADL
                     QWLARAYQFAAKDYDQWTKAPHLAGDTGLSRYFDEGNGPVPEIADDPTNYYRGVANFF
                     LTHEGEKSAHLVMVGAKQSQAAALPPVFNIEFCPPGAAKNQNAGCHQDRVELTADFYK
                     GDRSVRESGFDISFRFGPYGANTHRFAPVCLNSLLYKTEKDLEQMSTLLGHADQARAW
                     ANKAAQRREKIIKYFWNEHRGMFFDYNFATGRQSTYDYATTFYPLWVGLASDGQARAV
                     DANLHVFEQPGGLAMSTHESGGQWDYPYGWAPIHLLTVEGLRRYKFNADADRISFKFL
                     STVLQNFRREHTIREKYNVVSKSDVTHVIVGYHQNVIGFGWTNGTFLELLHESPGLAA
                     RLKEE"
     gene            complement(11546..12541)
                     /locus_tag="VN788_11715"
     CDS             complement(11546..12541)
                     /locus_tag="VN788_11715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU21239.1"
                     /translation="MNGKIKLGIVILIALVVIAAAAVTWGVLHRINKPISLMGAIIKQ
                     DTDPKKQSPITDVNVSVADGLALASVRSDFSGYFKLQLRPRLKPGQPITLQFRHKDYK
                     PLDVKTTLDRKLYVIPMVPLHQEVTAAAPSNRPLIPLSNVFVRYSTESTTSDNIGTGL
                     RTFQVVNAGNVPCNSHAPCSPDGRWKGAIASTSLDAGVGNTYDDARLSCIAGPCPFTR
                     IVSDNFSHGGRYISVSILNWSDTTTFLLQAEVFRIQISNIVRESYPVIFGNSLNFTLP
                     ASAEGPSLEAEESGQNIVFPLGPSPQLSWADCNVRVGKDQSKSYRCELKPGFQFR"
     gene            12943..>13487
                     /locus_tag="VN788_11720"
     CDS             12943..>13487
                     /locus_tag="VN788_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014074989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21240.1"
                     /translation="MAADLKSPRRIATEEAFSIPEVAAGLRGVARAPGNSLDLILVKR
                     IYDAPKDDHPQMAALLPGLLDLEEKRLPDMDKNGVAMQLLSLTAPGVQMFDADTATEL
                     ATLANDRLAEVISRHPTRFAGLAAFAPQSPKRAVKEMERAIKTLKLNGFIVNSHTNND
                     YFDNPKFFPILEAAEALDGCI"
BASE COUNT         2828 a         3741 c         3946 g         2972 t
ORIGIN      
        1 aacgcaatca tcctgagttt gccgccccgc tcccatgtag gaagcattgg ccgtgacgga
       61 ttctgccttg ccgatatctt tcaccaactg gccatagtag ggcggcagtt ccgctgaatc
      121 ggcacccgct ggccgcaagg agctgctcgc cgtatccatc tcattggcaa gctgcgacac
      181 cgtgctccac tctttcgggg ttggcccttc gtccctcgcg attgcgttaa agttcgcgaa
      241 aaaatcggcg cgttgctgcg gcgtctcgcc cgctgcgttg aattgatccg ataaaatccc
      301 tttgagcttt tcgtccgagt cggcgttgcc ggagcgcgcg gccgtccaca ggtctcgaat
      361 attctgctga acctcgggat cgtttttgta gcgcgaatca atggacatct cccactgatg
      421 tttcatcagc gccttatctg cgtccgatgg attcatcgtc tggagggcgg gcgacgtgcc
      481 gtcaactcgg gccgggtcca caacggttgt gctggcgcgc cgcaggtcaa cagcgtcaga
      541 gtctggattc ggatcggctt ttccgagcgg agcgttgcca gtagtagtgc catcaaagtc
      601 ggggtttcca atgccgtcca ataggtgtga acgagattgt tgggctgaat tggcagatac
      661 agccacggct ctgtttccct caactgcggc tgccggtggc tgcgtatcag cactttgatt
      721 ttgtccgatc gcctgatcga tttccttcac cgtctccgcg tccggttgtg agctcttagt
      781 ttcaaggatt gcattgggcg caggttcaac gttgttcgat cctgaatctt ggatcaggtc
      841 attgttagcg aaatcgtcta actggcgctt cacttccgca tctgatccgt tacctagcac
      901 gttatccagc gattgcagag tttgcgcctg ttcgggattt aaatgggttt tcgtcgtgcc
      961 gcgcaaatct accgcgccag gatcggaatc caagtccact aaatttagcg cgggtccatt
     1021 cgcgcctccg gtcccggtta gagcggaatt gagagttccg gcgctggctg gagcactttc
     1081 cagaagttgt ccgagggcgt tgctagtctg ccctgccatg gcaacgtctt tcaatttttg
     1141 cttcgccaac gcgagattgc ggagaatgtt tccatcaccg ggagtaatcg caagagcttt
     1201 ctgaaattcg ttgatcgcac cggcataatt tttctgccta aaaaggtaga tgccgctatt
     1261 gttgagctgt cgggcggcca attgccgctg ctgcgcggca gtgtccggtg ggctaggagg
     1321 tgcgccgaac aacaattgat gaaggccctg accaaccatg tagccgccga gcattccgat
     1381 ctgcatacca acctgttgct gaggtgtaag gttgagattc ggagagggcg cgctggtggc
     1441 gacagaattc gatccgccac tgcaggtcac ctggcctgag ccgacagaac tcccaggaca
     1501 gatgcatccg ttctgcgcaa cccatgcatt gtaatttgac gcagctgtcc ccccggaatc
     1561 ggtgcacgct ttgtacatcc gcgcgacttc cgcggcctca ccctgcgcct tggttgcgcg
     1621 cggaaagccc agcattaccg ccaggcagac gatgcttccc atcagtgtga aagctctgtt
     1681 tttcaaagta ggaactctcg accggtgtct gcgcgggctt gtcgccgctg agccgatttt
     1741 gaagctgggt ctcgccgtcg caaatagtac tgacggccgt ttgacagaga atgtgcttcc
     1801 ttgacaacac ccgcaacgct gcggttccga acgcccctgc gcgagccgcc attgcgacgg
     1861 ggcgtattcc acgcatacga ctcgctgtat agaaggggcc taaactcttg tcgctgttgc
     1921 cgtcgtcgcg agccccccgg ctgtatctct tgcccacagc gagacggttt ttccgtcgct
     1981 ctcgggcttg ccgcacagta caaacggctc cgtatcgaat agcgggttca ctgctcgaaa
     2041 cgcgaagtgc tctaccgtgg cattcggcaa attgcgccgg attagatcga ggagtagtgt
     2101 cgcaatgagc gggccgtgca ccaccagccc cggatagcct cccacttccg tcgcgtagcg
     2161 gcggtcgtaa tgaatacggt gcgcgttgaa tgtcagcgcc gaataacgga agagcagcac
     2221 atcgtctggc tggaccactc gttcccacgc tgcgtttgac ggagccggct gcgttgccgc
     2281 cgtgcccacc tggtccgccg tcgcgttgtc gcgataaaca atatcctgct cctcgctcag
     2341 cgcgattccc tcggcgtagc cgatttcgtg tcgcacgatc acgaatatca gcggcccgct
     2401 acgtccttcc ttctgttgta catccagaat gcgcgaagtg cgcgtgtacg tttgccccac
     2461 acgcaacggg tgatgaaatt cgaatcgccc acccgcccac atgcgcaggg gcaaaggaac
     2521 gggcggcaaa aagtcacctg tctttgcgtg tccatccggc ccaagttccg actgcttgtg
     2581 aatcggcagg aaccacatcc agtgccacaa aggcggcacc atatcgccag gacgcggcgc
     2641 tgaagcatca atatcgagcg tcgcggatag cgctgccacc ggcatcgaag ttatctgatc
     2701 cgttcgcgtt tcggttttcc ccacccacgc ttgaaattcc gcagcggtca cgtcttcgct
     2761 ctcctctttt tcttgccacg gcacattacc acgttccgcg cgccacaaag cccgcgtgat
     2821 acactccgcc tcctgaccag ctacgaacgc gaaatccgca tgcacagcag cgcacaaatt
     2881 acggcgcaaa agaactctga cagcacaatt cccatcgctg ttggctggcg catgtggatt
     2941 cccgtggtcg cgatgatgac gtgctcctgg ctttcctacg tcgatcgcca ggcgctcgcc
     3001 gtaatttcgc caatgatttt gcgcgacacc ggcctgagcg cggcgtctta tgcggatgcc
     3061 atctccgttt tttccattgt ctacatgatc gcgaatccgt tgtggggatc gctcctggat
     3121 tacgtcggac tgcgcgcagg catgctcgtg gccgtgggca tttggacaat cgcgagcgcg
     3181 tcgcacgcct gggtgatggg ctttctgggt tttgcaatgg ctcgcggact tctcggcttc
     3241 ggcgaaggcg ccacttttcc cggcggcttg cgaaccgctg ccgattcttt gccgattgac
     3301 cgccagtcgc gcggcatggg aatttcctac agcggtgcgt ctgtcggagc cattttcgcg
     3361 ccgctcatca ttactccgat cgcgctgcga ttcggatggc gcgccgcgtt catagccacg
     3421 ggcgcacttg gtgcgggatg gctcgccatc tggtggtttg tggcccggcc accattggta
     3481 cgcgccgtga atcgaagcaa gcgcaaagtc acgtggccca atttgctcga gcgccggttc
     3541 tggctgctag tggtgggcat gggacttggc ggcgccgcgc ttggtcccac gctgtacctc
     3601 agtccacttt atttgaatcg tgttctaggg ctcacccaag ggcaactcgg aagaattttg
     3661 tggattcccg cgctgtgctg ggagctagga tattatttct ggggctggat cggcgatcgc
     3721 ttcgtccgcg aaaatccgcg gcccattcgt ttgtacattc tgctaacgat tctagcgctg
     3781 ccgctttcgc tcataaccga aaccacttcc tgggtcgccg ctctggcttt tttttcttgg
     3841 gccatgttca tcgcggttgg cttcatcgtc atttcgctgc atctcagcgc gcgcgcttat
     3901 ccgcgcgatg aaactggaat ggtcgctggg attggttccg gcgcgtggtc ggcggtagtc
     3961 gcgattcttt tggtgatcta cggacgctgg ttcgaccgcc agttatacgc agccacattc
     4021 gtttccttga gcctcgttcc cgttttaggg acagcactgt ggtggtggat cggccgcgaa
     4081 ccatcgcgcg ccgcacgtta atcgcccatt cgcatgataa acgggttcgg cgcgttgccc
     4141 acaatgtcta tccaaacgct ttttgtttgc aggtattccc gaatcgcatc gatgccgctt
     4201 tcgcgaccga ttccagaacg tttgtatccc ccgaacgggg acatgtaact cactgcgcgg
     4261 tacgtgttca cccaaactgt tcctgcttcc aattgctcgg acatcagcag cgcgcgccgg
     4321 atgctgcttg tccacactcc cgcagccagg ccataaaccg ttccgttcgc aattttcacc
     4381 gcttcttcct cggtatcgaa aggaatgatg gaaagaaccg ggccgaatac ttcctcttgt
     4441 gcaatgcgca tgtcgggccg cacgccggtg aaaattgtcg gttcgacgaa ccaacccact
     4501 ccgcactccg gccgctgggc tggcgtgcca cccagacggc acaccgcgcc ttcttctttt
     4561 gcgattcgga tgtaatcgag gactttttcg tattgcggcc gagtggtgat cggaccgact
     4621 tgcgtggccg gatcaagcgg attgcccatg cgcgcggtct tggccatcgc caggagtttc
     4681 tccacgaatt tgtcgtgaat gggacggtga atcagcgcgc gcgatccggc aatgcaagtt
     4741 tgtcccgagg ccgcgaaaat tccggagacg atgcctttca gcgcttcctc catatcggca
     4801 tcgtcaaaaa cgatgttcgc cgattttcct cccagttcca gagtgacgcg tttgagtccg
     4861 cgcgcggcca gttgataaac gtgctcgcca gtgcgatcgc cgccggtgaa agcaactttc
     4921 gccacgtcgc gatgcgcgat gagcggttcg ccaacctcat tgccgtatcc ggtgacaata
     4981 ttgaccacgc caggcggaaa gcctgcgcgt tcgaatagct cgccgaatgt gagcgccgaa
     5041 acggacgcaa attcggacgg cttccacacc accgtattgc ccgcggccag agcgggcgca
     5101 agtttccaag tcgccagaag cagcggggaa ttccacggag tgatcgccgc aaccacgccc
     5161 accggttctt cgcgcgtaaa attgaatacg ccgggcttgt cgatgggaat tacgcggccc
     5221 tcgattttgt ccgcgaggcc cccgaaatag cggaaccatt gcggaacgta tttcacctgg
     5281 ccgcccattt cggcaatcag ttttccattg tcggtggtct caatctcacc gagcttggca
     5341 gcctccgccg caattagatc ggccaagcgg ctaagaagag ccccgcgcgc cgtggcggtg
     5401 agcttccgcc aagcgtcgcc gtaaaaagcg catttggctg cggctacggc ttggtcaata
     5461 tcgtctttgg ttccgcgcgg gattagcgcc cacggcttcg cggtgtatgg attcaccgac
     5521 tcgaaccact cgccggaagc cgggtcgacc cattttccgc cgaccagcat tttgtgtttt
     5581 ttcatctcag gcatcgatag ttgtcctcat cctcggtcgc tcgctttctc cattacaaat
     5641 tgcgggtcgc attccgcccg cttcgacact tctcggtttg gtgtcgagta gactgcaccc
     5701 cgatcctttg gtttgtggaa cttacaagga attctactcg gcagcagtgt cgaatagaat
     5761 ctgacgaatc gaccggtaag catagagcgc aaagattccg tgagctgaag cgtttcgccc
     5821 gcctcgccat ttcgtttcat ttgctcagct attttcatac gagcaaagta gctaatacgc
     5881 ggaaaccgcg caagcggttg ttggtgtaaa gaattttcga gacgccgcga tcaattgcta
     5941 ggcggcggcc cgatgaggca tgatacgcgt tctgaaaaca tagattcggc gggtcactca
     6001 gagcaagaac gctagagtcg aagcgagcaa ggttaggggg agagatggcg aagcagcctt
     6061 tgggatttgt ggggaccgga cgaatggggt cgcggatggt gcggcggctg attcaggccg
     6121 ggcacgaagt tacgatttat gacacgagcg aggcggccgt tgcgccactg gtcgagattg
     6181 gtgcgaagcg cgctgcttcg cctgccgcag tcgcttcggc tgcggagatt gtgtttgcca
     6241 gcgtgccaac gcctcccatc gtgcaagctg tggcgctggg ccagaacgga atcatcgagg
     6301 gcaaacgcgt gaaaattttc atcgacgttt cgaccacggg cgcgactgtt gagaaactgg
     6361 ttgcggaggg attggctgcc aagggcatca ccgccgtgga cgcgccggtt agcggcggaa
     6421 ttaccggagc ggaaaaaggc acgctggcag tgatggtctc ctgtcccgag gatgtgtttg
     6481 ccacgctgaa gccgatcttg gaaatcatcg ggaagctttt cttcgtgggc accaagccgg
     6541 ggcagggcca gacgatgaaa ctgttgaata atttgctctc ggccacggcc atggcgatct
     6601 catcggaagc cgtagtgatg ggagtgaagg cggggctcga tccgtcgctc gtcatcgatg
     6661 tgttcaacgc gggaacggcg cgcaacagcg cgacgcagga caaaatcaag caacacgtaa
     6721 tcccgcgaac gttcacctac ggcttcggaa tcggcctgct gaataaagac attcgattgt
     6781 gcatggcgga agcggatgcg ctcggcgtgc cgatgattgt cggtagcgcg gtgcggcagc
     6841 ttttgtcaat tgccacggcg agtgaaggtt tcgacgcgga catgaccgaa atggttaagc
     6901 cggtggaaga gtgggcggga gtgcgagtgg ggaaaggctg aacgaaagcg gtcggctcgg
     6961 aaatgacgtc gcagccttcg ggatggcgag aaaacggcga ggcgcttggt tcagcgtgat
     7021 cgccgatgcg tgcggtgaaa ttaaaactga aagagaggat ttatatggat cagaaaacgt
     7081 ttgagcgtgg caaagcgatt cgaagctcgg ttttgggcaa ggagtatgtg gacaatgcgt
     7141 ttcaaacggc ggacgatttc aaccgccctt tgcaggaatt agtgacggag tattgctggg
     7201 gcgcgatttg ggggcgcgag gagcttccga aaaaaacgcg cagcatgctc aatctggcga
     7261 tgatcagcgc gctgaatcgt ccgcacgaat tgaagtcgca catcaagggc gccctcgtga
     7321 acggcgtttc aatggtggag atacgggagg ttttgctgca ggtggcgatt tactgcggcg
     7381 tgccggcggc ggtggattct ttccggatcg cgcgcgaagt tttcgcggag cagaaagaga
     7441 aagcgcccaa ggcatagcag gcgaggtaat tcaatttggc gatagaaccg tttgaggttt
     7501 ttgcgattcg gtatgcgcat ctgggcgggc ggcaccccgg tgagaatttt gtactggccg
     7561 atccgcacga gtttgcttcg gatttggatt atttcgtgtg ggtgctgcgg cgcggcgatt
     7621 cgagcttcgt gatcgatacg ggattcggga aggaagcagc ggctcgcagg cggcgcgagt
     7681 ggctgcgctc gcccgtggag agcttgcggc tcgtgggggt gaatccagat caggtgggcg
     7741 acgtgatcat cacccatttg cattacgatc atgcggggaa tctcgataaa tttccgcgtg
     7801 cgcgttttca tttgcaggat cgcgatatgg cctacgccac ggggcgctgc atgtgccacc
     7861 aaacgctgcg cgcgcctttc gatctcgaaa atatgctgga gatggtgaag cacgtctatt
     7921 cggaccgagt ggaatttcac gatggcgatg cggaacttgc agctgggctg agtctgcacc
     7981 gcgtgggcgg gcactccgcg ggactgcaaa tcgcgcgggt ttggacgagg cgcggatggg
     8041 tggtaatcgc gtccgatgcc acgcacctat acgcgaattt cgaacagcgg cggccatttc
     8101 ccatcgtgta caacgtggcg gaaatgctgg agggttttaa gatgctctac aggcttgccg
     8161 attcgccaga tcacatcgtg ccgggacacg atccgctggt gatgaaatat tatccggctc
     8221 cgaagccgga gctcgccgga attgtggtgc ggttggatgt ggcgccgatc aagtcgcctg
     8281 cctaagcggc cgctacgagt ccgtgacggc gctagcagcg actaaagccg agcggtctta
     8341 ggcgcgacgt ggcgcttgcg ccaaaactcc tcgatttctt ccagagtacg tcctttggtt
     8401 tccggcacca gatagtacga aaaaaaccaa gcgccgatgg cgaatattcc gtagagccaa
     8461 aatgttcggc tcgcgcccat tagttgaagc agtgtgagga aggtaagcga aaccagaaga
     8521 ttcgcgcccc agttgaaggt ggcggaaaga ccttcggccg agctgcgaac cggcagcggg
     8581 tatatttccg caattagcag ccagaaaatc gggccgaggc tgatagcgaa agatgcgaca
     8641 taaagcatca tgcagatgac ggccaccgta gcgagcgagc cgccgtgcat cggtgaatga
     8701 aaagcatatc ccagggcgaa taacgtgatg atcattcccg cgattccagt gagcagcaag
     8761 gggcgacggc cggcgcggtc gatgagccac atggaaacga tggtcatcac gacgttgacg
     8821 accccgattc ccgccgtagc cagaatggcg cccgacgctg aaggaattcc cgcggatcga
     8881 ataatcagcg gcgcgtaata gatgacggtg ttgatgccgg tgatctgttg aagaatcgca
     8941 aggccgatgc cgacgaccag cgcagctcga agagagggat gaaaaagatc ggatagacgc
     9001 ccgtgctctt cggcatgctg gatgctcgct tcaatatctt tcatctcggc atccgcgccg
     9061 gcagcggtgc gaatgcgccc caaaactttg cgtgccgctt cgagatggcc tttccttgca
     9121 agccagcgcg ggctttcggg cagaagaatc atgccaagag caaagacggc ggcgggcacg
     9181 accgccagtc caagcatcca acgccatcct tggatggccg cgaacgcgta atccacaacg
     9241 tatgccagaa gaattcccac ggtgatggcg agctgaaaga tcgaaaccgt ccagccacgg
     9301 gccgcgggcg gagaaacttc cgagatgtac acgggaacgg tgccggagga aagaccgatc
     9361 ccgaggccga caataatgcg tccgataatc agtaccgccg gtgacgaggc cgcggcacaa
     9421 atgatggcgc caatcgcaaa gatcacggct gtggcgatca gcagtctgcg gcgtccatat
     9481 agatcggcta ggcggccgcc gccaagggct ccgatggctg cgcccaccaa tacgccgctg
     9541 acgattacct cgagcgcgaa ggacgaaagc gtgaaatctt ttccgaggaa cagctcggcg
     9601 cctgaaatta cgccggtgtc ataaccaaaa agcaggccgc cgaggcccgc gaacgcggcc
     9661 gcaacgtaaa cgaaaccgcc tgaactgccc ggcggggtgg aaacggtggt ggcgctcatc
     9721 agctttattc ctccttgagt cgagcagcta gccccgggga ttcgtgtaat agttcaagga
     9781 aagttccgtt cgtccaccca aaacctatga cgttttgatg atagccaacg atcacatgcg
     9841 tcacatcgga tttcgaaacc acattatatt tttcgcgtat ggtgtgctcg cggcggaaat
     9901 tctgcagcac ggtgctaaga aatttgaaac tgatgcggtc ggcgtccgcg ttgaatttgt
     9961 agcgccgcag accctcgacc gtcagcagat gaatgggagc ccatccatag ggataatccc
    10021 attgaccgcc gctctcatgg gtgctcatcg ccagcccgcc gggctgttcg aagacatgaa
    10081 gattggcgtc cacggcgcgc gcctggccat cggaagcgag tcccacccac aaaggataga
    10141 acgtggtggc gtaatcatat gtcgattgcc ggccggtagc gaagttgtag tcgaaaaaca
    10201 tgccgcgatg ctcgttccag aagtatttga taattttttc tcgccgttgc gcggctttgt
    10261 tcgcccatgc gcgagcctga tccgcgtgac cgagcagagt gctcatttgc tcgagatctt
    10321 tttccgtttt gtagagcaag ctattgaggc agacgggcgc aaagcggtga gtgttagctc
    10381 cataaggacc gaagcggaag cttatatcaa agccggactc gcgcacgctg cgatcacctt
    10441 tataaaaatc ggcggttagc tcgacgcgat cctgatggca cccggcattt tgatttttcg
    10501 ctgcgcccgg agggcaaaac tcgatattga agaccggcgg taaggccgca gcttgcgatt
    10561 gcttggctcc gaccataacc agatgtgcgc ttttttcgcc ttcatgagtc aggaaaaaat
    10621 tcgccacgcc gcgatagtaa tttgtcggat cgtctgcaat ttcaggaacc gggccgtttc
    10681 cctcatcgaa atagcgagat aggccggtgt ctcccgccag gtgcggcgct ttagtccact
    10741 ggtcgtaatc cttggcggcg aattgatacg ctcgcgcgag ccattgaagg tccgcatggc
    10801 cggcaccgcg atccgcctgg taaacggcga gaatcatcga gctaagaaaa ggcggctgcg
    10861 aacggccgag gtaataggtg cgattggcgt tgaggactcc tccatagtgc tgaatttcgt
    10921 aaaaaaagtt ttcgaccatt cctttggcga ggtcgcggtg attgtcgcga agcaagccgc
    10981 gaatgatgaa atagctgtcc cacccgtaca tttcgttgaa ccgcccgccg ggaacgacgt
    11041 aaggattttc aagataaagg agtcccgccg gctggatttt tccggcatct tcttcggaaa
    11101 gatttgctgg aagatgcttg atctgcagat ggcagcgctg ctgcatttga gccaacgcag
    11161 gagaaatgga aacgtccgcg ggcaaataca gagtgggttt acctttggtc ttgacgtcct
    11221 gataggtggc gcactcggtg agtgagcgct tcaagccggt ccaatcgtcg gaaatgtaat
    11281 ggagaatatc gtcgagcgat tgaaaagaat tttcggaagc ggcggtctgg ggcggcggaa
    11341 gagcggtgga ggcgtggctc gctaaagtcg cggcgatcag aatcgctgcg aatagcggaa
    11401 ggcacaaagc acgaaaatat ctggatccac caaccctcat ggttccaccc gacttcgcgc
    11461 actcaaccgt actcaacgat agactggatc aggctaaagc gaaagcagcg cgtgggcagg
    11521 ctggaagaaa tcccgcgcga gaattttacc gaaactgaaa acccggtttc agttcacagc
    11581 gatacgattt ggattggtcc tttccaactc tcacattgca atcggcccaa ctcagttgcg
    11641 gcgacggtcc caacgggaaa acaatatttt gtccgctctc ctcggcttcc aggctcggac
    11701 cttccgcgct cgccggaagc gtaaagttca gcgaattgcc gaaaataacc ggatagcttt
    11761 cgcggacgat gttgctgatt tggattcgaa atacttcagc ttggagcaga aaagtcgtgg
    11821 tgtccgacca attgaggatg gatacgctga tatatcgtcc accgtgagaa aaattatccg
    11881 agacaattct cgtgaaggga cacgggcctg caatgcagga aagtctcgcg tcgtcatacg
    11941 tgtttcccac gcccgcgtcc agcgacgtgg aagcaattgc gcctttccat cggccatccg
    12001 gcgaacacgg cgcgtggctg ttgcaaggaa cgttgccggc gttgaccact tgaaatgtcc
    12061 tcagccccgt gccgatgttg tccgacgtgg tgctttccgt cgaataacga acgaacacgt
    12121 tggagagcgg aataagcggg cgattggatg gcgccgcggc ggtgacttcc tgatgaagcg
    12181 ggaccatggg aatcacgtag agtttgcgat ccagcgtggt cttgacatct agcggtttgt
    12241 aatctttgtg gcgaaattga agagtgatcg gctgcccggg tttgagccga ggacgcagtt
    12301 gcagcttgaa gtagcccgaa aaatccgatc gaacactggc aagagccaga ccgtccgcca
    12361 cgctaacgtt aacatccgtt attggagatt gttttttagg atcggtatct tgcttaatga
    12421 tagcgcccat cagcgaaatg ggcttgttga tgcgatgcag cacaccccaa gtgacagcgg
    12481 cagccgcaat tacgaccagc gcaatgagta tgacaattcc gagtttaatt ttgccgttca
    12541 tccgagacag ccgtcatatg ttagcaaaac tggcgcgatg gattccaagc aacgcacgcg
    12601 cattgctcgc gccgatattt gcgcgcggaa tccttcgagt ttggcacatg aaatccttct
    12661 agctagaaac aatttcctgc tcaaaacggc ccagcaagtg ccttgaacca cctcattgcg
    12721 ttacgttttc cagccgattg ccaaattcat ggttcgcctc tttcttcgac cgatcaggac
    12781 cgcatgccac ccgggacttt gtaacctgtt tccgcttttt ccgctagctc tcttggcccg
    12841 aaatctcgaa accagcagcg gcacttgcat tttgaacggc ggcgcgccaa gctaccagcc
    12901 cgcgacacag caaaaatagt ctcgacgaaa ggaaaaaccc tcatggccgc tgatttgaag
    12961 tctccgcgcc gtattgccac ggaagaagct ttttcgattc ccgaggtggc ggcgggtctg
    13021 cgcggcgttg cccgcgctcc tggaaatagc ctggacctta tcctggtgaa acgaatctac
    13081 gatgcaccga aggacgatca cccgcaaatg gcggctcttc ttccggggtt gctcgatctg
    13141 gaagagaagc gcctgcccga tatggacaag aacggcgtcg cgatgcagtt gctttctctc
    13201 accgcgccgg gcgtccaaat gtttgacgcc gataccgcca cggaattggc gacgcttgcc
    13261 aacgaccgcc tggccgaagt gatcagccga catccgactc gcttcgccgg cctggcggca
    13321 ttcgcgccgc aatcacccaa gcgcgcggtg aaggaaatgg aacgggccat caaaactctg
    13381 aagctgaatg gctttatcgt gaactcgcac accaacaacg attacttcga caatcccaag
    13441 ttttttccga ttctggaagc ggctgaagcg ctcgacggct gcatcta
//
LOCUS       DATNDT010000366         4126 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_373636, whole genome shotgun sequence.
ACCESSION   DATNDT010000366 DATNDT010000000
VERSION     DATNDT010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4126)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4126)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4126
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373636"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..715
                     /locus_tag="VN788_11725"
     CDS             <1..715
                     /locus_tag="VN788_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HXU21241.1"
                     /translation="AVLENVDIDPDTRRPNYDSDRYTENTRAAYPVEFIHNAVVAGMG
                     GHPKNVVLLTADAFGVLPPIAKLTPEQAMYHFVSGYTSKLAGTEAGLGKQPQPDFSTC
                     FAAPFLPLPPKVYAKMLGERLKQHNAQCWLVNTGWVGGPYGVGSRMKLGYTRAMVRAA
                     VDGNLAKGEFEIEPAFGLTIPKSVPDVPSELLKPRNLWKNKDDYDKKASELAAKFAEN
                     FKRFDVPESVRAAGPKSIG"
     gene            804..1064
                     /locus_tag="VN788_11730"
     CDS             804..1064
                     /locus_tag="VN788_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21242.1"
                     /translation="MFSRKIKVEYEHEGERKSCELKWLDSFSMRNFTNDAVFDDTLPI
                     SDGLIEAGTRVPTEQLRAAMEDWFHRKSYIGKQDRLKLKEIG"
     gene            complement(1195..2058)
                     /locus_tag="VN788_11735"
     CDS             complement(1195..2058)
                     /locus_tag="VN788_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21243.1"
                     /translation="MRKLPLIVLVFLFISSNLAHSQAPSPPTAEAIMARVAANQDRSE
                     QLRTQYVYRQHIHVASRKTNGKLMREETADYDVLPDASGSKKELKKLDGRYLDKGKYV
                     SYATEAASKGDGLDAGLVNSFREDLTNEKSKDGVGRDLFPLTSSEQAKYSFQLLGEQT
                     LNGRSVYHLSFRPRRIGPAKHEWDDAEWAGEAFIDKEDFEPVNVYTKLAERIPLFVRT
                     VFGTDLPGLGFSVTYKRQPDGVWFPSSFGTEFRIHAVFFINRVITVSLQNSDFERTHV
                     DSKITGVAAIQ"
     gene            complement(2214..3449)
                     /locus_tag="VN788_11740"
     CDS             complement(2214..3449)
                     /locus_tag="VN788_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HXU21244.1"
                     /translation="MQKIKTTCCVVGGGPAGMMLAFLLGRAGVRTVLIEKHADFLRDF
                     RGDTVHPSTLELLYELGLLEDFLRRPHQELGRVAAQIGDTSLWVADFSRLRTHARFIA
                     FVPQWDFLSFLAEHAQKYPEFQLLMQTEAVDLVEENGRVTGVRAKAPNGDLAIAADLV
                     VGTDGRNSIVREKAGLSVEEMGAPIDVLWLRLSRETSDPGRVLGRIDRGRMMAMLDRG
                     EYWQCAYVIPKGSAKTLKARGIQNFRDEIARLVPYLGSRTAEIKDWDDVKLLTVKVDR
                     LKQWYKPGLLCIGDAAHAMSPIGGVGINLAVQDAVAAANILAEALAKGFVPILEQVQR
                     RREFPTRITQWFQILAQDRVISQVLASSEPIQPPFILKLMQRWPVLQGIPARAIGLGA
                     RPEHVNTRDVHGVKETPQG"
     gene            3535..3909
                     /locus_tag="VN788_11745"
     CDS             3535..3909
                     /locus_tag="VN788_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU21245.1"
                     /translation="MLSAGKLIGFVPTRDSTRAREFYEGKLGFQFVSDDPFALVMRAG
                     KNLIRIAKVKDFTPAQYTVLGWEVENIEVIVKWLQERGVVFEKYPFVQDQTLGIWTAP
                     NGDEVAWFKDPDGNVLSVSRHV"
BASE COUNT          898 a         1244 c         1134 g          850 t
ORIGIN      
        1 cgccgtgctg gaaaacgtgg acatcgatcc cgacacccgc cggccgaact acgattctga
       61 tcgctatacc gagaacactc gcgcggctta tccggtggaa ttcatccaca acgccgtggt
      121 tgccggaatg ggaggccatc ctaagaacgt tgttttgctc accgccgacg ccttcggagt
      181 gcttccaccc atcgcgaaac ttacgcccga acaggcgatg taccacttcg tatccggcta
      241 tacgtcgaaa ttagccggca ccgaagccgg actaggaaag caaccgcagc ccgatttctc
      301 cacctgcttc gccgcgccct tcctgccgtt gccgccgaaa gtctacgcaa agatgctggg
      361 agagcgcctg aaacagcaca atgcgcaatg ctggctggtg aataccgggt gggttggggg
      421 tccgtatggc gtaggatcgc gcatgaaact gggctacacc cgcgccatgg tgcgcgccgc
      481 agtcgacgga aatctggcca aaggcgagtt cgaaattgag cccgcgttcg gccttaccat
      541 tcccaaatcg gttccagatg taccgtcgga actgctgaag ccccgcaatc tgtggaagaa
      601 caaggacgat tacgacaaaa aagcctccga actcgccgcc aagtttgccg aaaatttcaa
      661 gagattcgac gtgccagaat cggttcgcgc agcgggacct aagtcgattg ggtaattgac
      721 taatcgggta attgagcaat tgaaacccgt cggccggcaa gggcatcccg ttttcaatta
      781 ctcaattacc aaatgccttc atcgtgtttt cccgcaaaat caaagtcgaa tacgagcacg
      841 aaggcgagcg caagtcctgc gaactgaagt ggctcgacag tttttccatg cgcaatttca
      901 ctaatgacgc cgttttcgat gacaccctgc ccatctctga cggcctaatc gaggccggaa
      961 cccgcgtccc caccgagcaa ttgcgcgccg cgatggaaga ttggtttcac cgcaagagct
     1021 atatcggcaa acaagaccgg ctgaagctca aggaaatcgg gtgattttgc gatcgggtga
     1081 cctcacccca gcaaccgcaa ccccggcgtt tgctggggac cccggtgatc gggtgatttg
     1141 aaatcacaaa atcgcgaaat cgcgaaattt tgtcagggcc cacgtgggtg agaattactg
     1201 gatcgcagcc acgccagtga tcttcgaatc cacgtgcgta cgctcaaaat cgctgttctg
     1261 gagcgataca gtaattacgc ggttgatgaa aaataccgcg tgaatgcgga attctgtgcc
     1321 gaagcttgat gggaaccaca cgccgtccgg ctgccgtttg taggtcacac tgaagcccaa
     1381 gccgggcaaa tcggtgccga aaaccgtgcg cacaaacaga ggaatgcgct ccgccagctt
     1441 ggtgtagacg ttcaccggct caaaatcttc tttgtcgata aatgcttctc ctgcccattc
     1501 ggcgtcatcc cactcgtgct tcgcgggccc aatgcgcctc gggcgaaagc tcaagtggta
     1561 caccgagcgt ccattcaggg tctgttcgcc caataactgg aacgaatatt tcgcctgttc
     1621 gctgctggta agcggaaaga gatcccttcc cacgccgtcc tttgacttct cgttggtgag
     1681 atcttcgcgg aagctattca ccagtccggc atccaggccg tcgccttttg aggccgcctc
     1741 agtggcatat gacacgtatt tgcctttatc gagatagcgc ccatcgagct ttttcagctc
     1801 cttcttcgat ccggaagcgt cggggaggac gtcgtaatcc gccgtttcct cacgcattag
     1861 cttgccattg gttttgcgcg aggctacgtg gatgtgctgg cgatagacat attgcgtccg
     1921 caactgctcg ctacggtcct gattcgccgc cacccgcgcc atgatagcct ccgccgtagg
     1981 cggtgaaggc gcttgggaat gcgctaaatt gctggaaata aatagaaaaa caagcacgat
     2041 gagcggcaat ttacgcataa cgtgacctgc cagaacacac agtttgacgc aaaatgagcg
     2101 tccgaattag cagttgtaag cgcaaattgt gggattggag gcgcaattac gagcggaatt
     2161 cttcagagag cacatctctc agacctagtt gcgaagcttc gaagtttctc atctcatccc
     2221 tgtggtgtct cctttacgcc atgcacatcc cgcgtgttca cgtgctctgg acgcgcgccc
     2281 agaccgatgg cacgcgcggg aatgccctgc aggaccggcc agcgctgcat cagtttcaga
     2341 atgaatggag gttgaattgg ctcactgctg gctagtactt ggctgatcac gcggtcttgc
     2401 gcgagtatct ggaaccattg cgtgatgcgt gttgggaact cgcgccggcg ctgtacttgc
     2461 tccagaatcg gcacgaaccc tttcgcaagt gcctccgcca agatattcgc tgcggccacc
     2521 gcgtcctgca ccgccagatt gatgcccaca ccaccgatcg gagacatcgc gtgcgcggca
     2581 tcgccgatgc acagcagtcc cggcttgtac cactgcttca gccggtcgac tttcacggta
     2641 agcagcttca catcgtccca atccttgatt tcggcagtgc ggcttcccaa atagggaacc
     2701 agtcgcgcga tttcatcgcg aaagttctgg atgccgcgcg ccttgagcgt tttcgccgag
     2761 cctttgggaa tgacgtaggc gcactgccag tactcgccgc ggtcaagcat ggccatcatg
     2821 cgcccgcgat cgatgcggcc cagcacccgc cccggatcgc tcgtctcccg cgagaggcgc
     2881 agccacagca cgtcaattgg cgcgcccatc tcttcaacgc tgaggcccgc cttctcgcgc
     2941 acgatggagt tccggccatc ggtgccgacc accaggtccg ccgcaatcgc cagatcgcca
     3001 tttggggcct tcgcgcgcac tccggtcacg cggccgtttt cctccaccag gtccaccgcc
     3061 tcggtctgca tgagaagctg gaactcggga tatttctgcg cgtgctcggc gagaaagctg
     3121 aggaagtccc attgtgggac aaaggcgata aaccgcgcgt gggtccgcag gcgggaaaag
     3181 tccgccaccc acaagctggt atccccgatc tgcgccgcga cgcggccaag ctcctggtga
     3241 ggacgccgca ggaaatcctc cagcagtccc agctcgtaga gcaactccag ggtggaagga
     3301 tgcacggtat cgccgcgaaa atcgcggaga aaatccgcgt gcttctcgat cagcacggtc
     3361 cgcaccccgg cccgccccag caaaaatgcc agcatcatcc ccgcaggccc gccacccaca
     3421 acgcagcagg tagtcttgat cttttgcatg ctgcacctat atggatttta ctgtcacggg
     3481 gcggaagctg cgccccttgc cggtctacaa tgtcatcttc gcaaggagag acgcatgctc
     3541 agcgccggca agctcatcgg atttgtccca actcgggact ccactcgggc tcgcgaattc
     3601 tatgaaggca aacttggatt ccaatttgtc agcgacgatc cctttgccct ggtgatgcgc
     3661 gcgggaaaga acctgatccg gatcgccaag gtcaaggact ttaccccggc gcaatacacc
     3721 gtcctcggct gggaggtaga gaacatcgaa gtgatagtga aatggctgca ggaacgcggc
     3781 gtagtgttcg agaaatatcc ttttgtgcag gaccagacgc tcggcatctg gaccgctccc
     3841 aacggcgacg aagtggcctg gttcaaggat cccgatggga acgtcctctc ggtcagccgg
     3901 cacgtgtgac gcgaagctgc gcagccgcgc cactccgcag cccgatttcg aaaggtttgc
     3961 caaactcaac tgaagccggc tcatcccttg ctcccctgcg gcaactcagg tataaagctt
     4021 ccattgccct cgggccctga gtgctggagc ttacgatgaa accaaaatgg aattggagac
     4081 tctgggcggg attcgccgtc agcgtgcttg ccctggcgat ttacgt
//
LOCUS       DATNDT010000367         8558 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3272276, whole genome shotgun sequence.
ACCESSION   DATNDT010000367 DATNDT010000000
VERSION     DATNDT010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8558
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272276"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..334
                     /locus_tag="VN788_11750"
     CDS             17..334
                     /locus_tag="VN788_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019936373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HXU21246.1"
                     /translation="MKKSEDLIAPVHPGEILREDFMKPLGLTVNKLALELHVPATRIG
                     EIVHGRRRITAETALRLARYFKTNAEFWLNLQNFYDIEVSRRTGKITEIEQQVRPAPA
                     LVG"
     gene            complement(651..1973)
                     /gene="purB"
                     /locus_tag="VN788_11755"
     CDS             complement(651..1973)
                     /gene="purB"
                     /locus_tag="VN788_11755"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXU21247.1"
                     /translation="MINRYTRPEMRHLWSDENKFQKWLDVELAAADVLAEAGTVPREA
                     AARLRERARIDVARILEIESKVRHDVIAFTVAVGESVADPEAARWLHYGLTSNDVVDT
                     AQALLLREASEQIQKGLAHFAEILERRAFEFKDTPQIGRTHGVHAEPITFGLKIANWY
                     DENRRDIVRFEAAAKEIAVGKIAGAVGTCAHLGPEIEAKICRRLGLAVAPISSQVIER
                     DRHAHFVSALAIIAASVERIALEIRHLQRTEVREAEEPFGGGQRGSSAMPHKRNPVGC
                     EQLCGLARVVRANATAAFEDIALWHERDISHSSVERVILPDSTTLVDYVLDRMSSIVA
                     NMKVFPERMLRNLNSTQGLVFSGQLLQDLVEHGAAREDAYKWVQAHAMAAWESESSFR
                     DRVAADPNITKFLDAKQLAHTFDLTRQLKSVDAIFARVFEKKSTAAPR"
     gene            complement(2123..2857)
                     /locus_tag="VN788_11760"
     CDS             complement(2123..2857)
                     /locus_tag="VN788_11760"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HXU21248.1"
                     /translation="MAPYLPIYYVLFYNSFMSAARKVHPSRHKCLIYEGDPSEQLPVI
                     VPLLREGLRDNWRCLYIGSPQMVSMIEAALRSAGTDVVHETKRGSLVLSSERNLLSDG
                     TFDPRLMLDGLRHLIDTAVGDGFKGLCATGDMSWELAHDRNFERLLEYEALLEQLFQN
                     RPLRGICQYHRDLVPAQAVRDALMTHRSAFIGSVLSIANPFYTPPEVLLSARTDVAKR
                     VEWMCQQVIRTATAQTRPHRRVSLAR"
     gene            complement(2965..3675)
                     /locus_tag="VN788_11765"
     CDS             complement(2965..3675)
                     /locus_tag="VN788_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXU21249.1"
                     /translation="MGSQTSSKGGAILRKTKKFQQLFGASQNSTHEQLPSQTTERLVF
                     CQKNTGTPTFQVEMIGDRDAALSRAASLLALGCMASGESPADYAVWVPAETSLIDRIT
                     RHAEELLHLGRGFSAEVELSPRENEVLKELLNHRVNKEIADALCISVRTVKFHVSSLL
                     AKFGVGSRWDLIQKARHMLGTKQVTDNFAGVQSSVQAAHAPAARDSAPASSSASTESR
                     QRQSSRVVPFRGGQLRPA"
     gene            complement(3992..5008)
                     /locus_tag="VN788_11770"
     CDS             complement(3992..5008)
                     /locus_tag="VN788_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HXU21250.1"
                     /translation="MPEKARPKLTRVIALGGGTGLSMLLRGLKEHIAPLTNGAAVPLA
                     IHVDAVVTVTDDGGSSGRLRREYSILPPGDIRNCIVALSPDEALLAKLFQYRFAGRGR
                     GLAGHSFGNLFLAALTDVTGDFPEAVKLSSNVLATRGRIFPSTAANVTLKATLDNGRI
                     ISGETRISRSTRPIRRVQLVPSTASPLAEVLSSIRKADLILLGPGSLYTSLIPNLLVR
                     GIAGAIANSRAQCVYIANVMTQPGETDGYSVADHVRAIYEHVGKQLFHAVVLNRSVPS
                     PAILRRYRRQGAEPVNLSLSELKKMGLKCIIGNLLQRGSIIRHDQRRLAKLVLTHFAD
                     YHSH"
     gene            complement(5027..5644)
                     /locus_tag="VN788_11775"
     CDS             complement(5027..5644)
                     /locus_tag="VN788_11775"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU21251.1"
                     /translation="MARTKSKTKSGTRSVSRRQSSRKRLKEKSLGHSLLRESNDQATL
                     QQLKAYEEGLKLFQQQKFARATEALERVTQGPNKELADRARVHLRICSQRLSRQSQPS
                     PRSAEDHYNQGIAMMNLGRWDDARVHLDRARKAAPKADHIVYAMAALDCLTGEAESSM
                     TNLKIAIQLRPDNRYRARNDEDFAFLQEDPRFTELLYPEREGASG"
     gene            5902..6489
                     /locus_tag="VN788_11780"
     CDS             5902..6489
                     /locus_tag="VN788_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020674751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HXU21252.1"
                     /translation="MPFINFPAREINCKLVYYGPGLGGKTANLQWIYEHTGSNQKGKM
                     VSLATETDRTLFFDFLPLDLGTVRGFRTRFHLYTVPGQVFYEASRRLILKGADGVVFV
                     ADSQEERLDANFETIDNLKEHLKDHNLNFDTIPYALQLNKRDLPNAMPVPELRKQLQL
                     KGEPVIEAVAVQGQGVFDTLKEVAKLVLNELKKNS"
     gene            complement(6504..6869)
                     /locus_tag="VN788_11785"
     CDS             complement(6504..6869)
                     /locus_tag="VN788_11785"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU21253.1"
                     /translation="MPLKITVRQSSGVSIIDLEGRLVIGPDADALKQRLDELARQGSR
                     HILVNLAKVTQLDSSGICSLLHGYTMMKQHGGSFKMLHPAGHAREVLEVMRLIEIIPT
                     LDDEKQAVASFSGANPAQS"
     gene            7023..7718
                     /gene="nfi"
                     /locus_tag="VN788_11790"
     CDS             7023..7718
                     /gene="nfi"
                     /locus_tag="VN788_11790"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015134155.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HXU21254.1"
                     /translation="MKHLRLHRWNVTPREAAQIQLELRERLELKDRLPVVHCVAGADL
                     AFDLARNKAIAGVVVYRFPEMEELERIWGESEIQFPYVPGLLSFREAPALLKVFERAK
                     NTPDLIFCDGHGYAHPRRFGITCHLGVLLDTPTIGCAKSLLIGEHDALPSGAGAWTEL
                     RDKEEVIGAVLRTRSNVKPIYVTQGHRISLRTALKFVKAVLDGYRIPRPTRDADHFVA
                     AVKKENKNAAAGV"
     gene            complement(7866..8210)
                     /locus_tag="VN788_11795"
     CDS             complement(7866..8210)
                     /locus_tag="VN788_11795"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21255.1"
                     /translation="MVLAASRASCTKFSIPRRAIQTLLLASSSFLHLCRLGVMSAGAS
                     THGTRSVLRRVYPRADIPVVQLSIDETQPPSSHYQIGARLAQLREENVLILSSGNLMH
                     NLHAYAWGRHVQ"
BASE COUNT         1869 a         2083 c         2780 g         1826 t
ORIGIN      
        1 ttaggaggca ttcgcgatga aaaagtcaga agacctgata gcacctgtcc atccggggga
       61 aatccttcgc gaagacttta tgaagccgct tggcctcacg gtgaataagc tggcactgga
      121 actgcatgtg cccgccacac gcatcggcga gatcgtccac ggacgacgtc gcatcaccgc
      181 agaaacggct ctgcgcctag cgcgctattt caaaacgaac gcggaattct ggctcaacct
      241 gcagaatttc tacgatatcg aagtgtcgcg ccgcaccggc aagattaccg aaatcgagca
      301 gcaggttcgt ccggctccgg cgctcgttgg atgatagcct tgcacggttt ttgcgttcgt
      361 agctcaggat agaatggcaa gttgcgttct cgcgagaatg aaaggaatac caagaacgga
      421 ttatggacag ccgagcagct tagtgctcct cggcaaaggg ctgtcgtgac taaattggtc
      481 gatgccatct tgttccgggt cttcgccaaa aaatccgcct ccgcgcccct gtaacgcaga
      541 cataagccaa gggttggagc tcgaaggtgt gttcgaggat gcgggggatc gttaggtggg
      601 aatgcgccag ctcaggatga caacgaagag gcgtgcgcat gcggcatcga ctagcgcggg
      661 gctgctgtgg atttcttttc gaatacgcga gcgaagatgg cgtcgacgga cttcagttgg
      721 cgcgtgaggt cgaaggtgtg ggctagctgt ttggcgtcga ggaatttggt gatgttgggg
      781 tcggcggcga cgcggtcgcg gaagctggat tcggattccc aggcggccat ggcgtgggcc
      841 tggacccatt tgtaagcgtc ttcgcgagcg gcgccgtgct cgacgagatc ctgcaaaagc
      901 tgtcctgaga aaaccaaacc ctgcgtggaa ttgagattgc gcaacatgcg ttcgggaaaa
      961 accttcatgt tggcgacgat ggaggacatg cggtcgagca cgtagtccac tagggtggtg
     1021 gaatcgggaa gaatgacgcg ttcgacggag ctgtgcgaaa tgtcgcgttc gtgccagaga
     1081 gcgatgtctt cgaatgcggc ggtggcgttg gcgcgaacga cgcgggcgag gccgcagagc
     1141 tgttcgcagc cgacgggatt gcgtttgtga ggcatggcgg agctgccgcg ctgtccgccg
     1201 ccgaaaggct cttcggcttc gcggacttca gtgcgctgga ggtggcgaat ttcgagggcg
     1261 atgcgttcga cggatgcggc gatgatggcg agcgcggaga cgaagtgggc gtggcggtcg
     1321 cgttcgatga cctgagaaga aatgggcgcg acggcgaggc cgagtcggcg gcaaattttc
     1381 gcttcgattt cggggccaag atgcgcgcag gtgccgacgg cgccggcgat ttttccgacg
     1441 gcgatttctt tggcggcggc ttcgaagcgg acgatgtcgc ggcgattttc gtcgtaccag
     1501 ttggcgattt tgaggccgaa agtgatgggt tcggcgtgaa cgccatgagt gcgaccgatt
     1561 tgcggcgtgt ctttgaattc gaaggcgcgg cgctcgagga tttcggcgaa gtgtgcgagg
     1621 cctttttgga tttgctccga ggcttcgcgg aggaggaggg cctgcgcggt gtcgacgaca
     1681 tcattggagg tgaggccgta gtgcagccag cgcgcggctt cggggtcggc gacggattcg
     1741 ccgacggcga cggtgaaggc gatgacgtca tggcgtactt tggattcgat ttcgaggatg
     1801 cgggctacgt cgatgcgggc gcgttcgcgg aggcgggcgg cggcttcgcg ggggacagtg
     1861 ccggcttcgg cgaggacgtc agcggcggcg agctcgacgt cgagccattt ttggaatttg
     1921 ttttcgtcgc tccagaggtg gcgcatctcg ggacgcgtgt aacggttgat caaaacgatt
     1981 ctcctgccag cgcgtctgag agttagggcc ggcctgaagg ccggccctac gaccgttgca
     2041 gacgccgatt cttgaacaca aataatactg ggaaattccc aaggatatga taaccgaaac
     2101 ggcgcgggcg ctggaatgcg aattagcgcg cgagcgaaac gcgacggtgg gggcgtgtct
     2161 gcgcagtggc ggtgcggatg acttgctgac acatccattc cactcgtttg gcgacgtcgg
     2221 ttctcgcaga caagagcact tcgggaggag tgtaaaacgg atttgcgatg ctaaggacgc
     2281 tgccaatgaa ggcgctgcga tgagtcataa gagcgtcgcg gacagcctgc gcgggcacga
     2341 ggtcgcggtg atactggcaa atgccgcgca gcggtctatt ttggaaaagc tgttcgagga
     2401 gagcctcata ttcgagcaga cgctcaaaat tgcgatcgtg agcaagctcc cagctcatgt
     2461 cgccggtggc gcagagtcct ttgaagccat cgccgacggc ggtgtcgatt agatggcgaa
     2521 ggccatcgag cataagccgg ggatcgaacg tgccgtcgga gagcagattt cgttcggaag
     2581 acaagaccag agagccgcgc ttggtttcgt gaacgacgtc ggtacctgcc gaccttagag
     2641 ccgcttcgat catgctgacc atttgagggc tgccaatgta gaggcagcgc cagttatcgc
     2701 gcaggccttc acggaggagc ggaacgatga cggggagctg ttctgatgga tctccctcgt
     2761 aaataagaca cttatggcgg cttggatgca ccttgcgcgc tgcggacatg aaactattat
     2821 agaagagaac atagtaaatg ggcaggtatg gagccaatcg gtaactccgg ggaacgcgct
     2881 caagcacgag aggcgggagg aatcggggct gggcgatgtg gccgccggcg gatgcagaac
     2941 agcggccaca tgaacccgag agattcaggc aggacggagc tgaccgccgc ggaagggtac
     3001 gacgcggctg ctctggcgtt gcctggattc tgttgaggca gaagaagaag ctggagcgga
     3061 atctcgagca gccggagcat gagcggcctg cacggaagac tggacaccgg cgaaattatc
     3121 ggtgacctgc tttgtgccga gcatgtgccg ggctttttga atgagatccc agcggctgcc
     3181 gacgccgaat ttcgccagaa gcgaagatac gtggaatttc accgtacgca cggaaatgca
     3241 aagcgcgtca gcgatttcct tgttcacgcg atgattgaga agttccttga ggacttcatt
     3301 ttcgcgcggg ctgagttcaa cctcggcgga aaagccacgg ccaagatgaa gaagctcctc
     3361 ggcgtggcgt gtgatgcggt cgatcaggct ggtttccgcc ggaacccaga cggcatagtc
     3421 cgcaggagat tcgccgctgg ccatgcatcc taatgcgaga aggctggcgg cgcgactcag
     3481 ggctgcgtcc cggtcgccaa tcatttcgac ctgaaatgtt ggagttccgg tgtttttctg
     3541 acagaacacg aggcgttccg tggtctgcga aggcaattgt tcgtgcgttg aattctgaga
     3601 tgcgccaaaa agctgctgga acttcttggt ttttctaaga atggctcccc ccttggaaga
     3661 agtttggctt cccaagccag cgggccgcgg ttattgccat ttgaaaaccg aagtccaccg
     3721 atttccaaac agcaagaaaa gcttcttgaa gagttttcgg aattccagga cgaacttggt
     3781 tccggcccag agtttgaaag gcaagttatg tgccgtggaa acagtaaaat agtaaaagta
     3841 ctagtatcag gggtttaaat gtaaggaata cagggaaata tgtatgcacg tttaagagga
     3901 atttgcacgg cgacggtaaa tttttcgcag gggcgatgcg aggatgaggc gtaagtatat
     3961 taaaactaca ggtttagtgg agtacaggcg ttcaatgaga atggtaatcg gcaaaatgtg
     4021 taagaacaag cttagccagg cggcgctgat cgtgacggat tatgcttcct cgctgcaaca
     4081 gattgccgat gatgcacttc aggcccattt tcttgagttc cgagagtgat aaattgacgg
     4141 gttcagcgcc ctggcgacga tagcgacgaa gaattgcggg cgaaggtacg gagcggttca
     4201 gaacaacagc atggaagagt tgcttgccga cgtgttcgta aattgcgcga acgtgatctg
     4261 ctaccgagta accgtccgtt tcgcctggtt gagtcatgac attcgcgatg taaacgcatt
     4321 gggcgcgaga atttgcaatt gcaccggcga tgccgcgcac gaggagattg ggaatcaggc
     4381 tggtatagag cgagcctggc ccgagcagga tgagatctgc tttgcggatg gaggaaagca
     4441 cttcggcgag gggcgaggcg gtggatggca ccaattgcac gcggcggatg ggacgagtgg
     4501 agcgcgagat gcgcgtctcg ccggaaataa tgcgaccgtt atcgagagtg gcttttaagg
     4561 tcacgttcgc tgcagtcgag gggaagatgc gtccgcgagt tgcgaggaca ttgctcgaga
     4621 gcttcaccgc ttcgggaaag tcgccggtga catcggtgag ggcagcaaga aagagattac
     4681 cgaaactgtg gccagcgagg ccgcgaccgc gtcccgcgaa gcgatactga aagagttttg
     4741 caagcagagc ttcgtcggga gagagcgcga cgatgcaatt tcgaatatca cccggcggaa
     4801 gaattgagta ttcgcggcgc aggcgaccgg agctgccgcc gtcgtctgtg acggtgacga
     4861 cggcgtccac gtgaatggcg agggggaccg cggctccgtt ggttagcggg gctatgtgct
     4921 ctttgagtcc ccgcaacagc atggacaatc ctgtgccgcc accaagtgca atgacacggg
     4981 tgagctttgg acgggctttt tcgggcatct cgggcgaacc gcgaatttag ccgcttgcgc
     5041 cttctcgttc cggataaaga agttcagtga agcgcggatc ttcctggaga aaagcgaagt
     5101 cctcgtcgtt tcgcgcgcga tagcgattat cgggacgcaa ctggatggcg attttcaaat
     5161 ttgtcatgga ggattcggct tcgccagtaa gacaatcgag ggcggccatg gcgtagacga
     5221 tgtggtccgc ttttggagcg gctttgcggg cgcggtccag atgtacacgg gcgtcgtccc
     5281 aacggccgag gttcatcatc gcaatgccct gattgtaatg atcctccgcg gatctgggcg
     5341 acggctggga ttggcgcgat aggcgctgcg agcaaattct gaggtgaacg cgggcgcggt
     5401 cggcgagctc tttgtttgga ccttgcgtga cacgttctaa tgcctctgtg gcacgagcaa
     5461 atttctgttg ctgaaagagc ttcaggcctt cttcgtaggc cttcagctgc tggagagtcg
     5521 cctgatcgtt cgactctcga agcaggctat ggcctaagct cttctcttta aggcgtttac
     5581 gactggactg acgccttgaa acggagcggg tgccactttt tgtcttgctc tttgtgcgcg
     5641 ccatagacgg agaatagcag cgatgcgcaa cggcgtcaac ttgtaggtag tgcaaggagt
     5701 aaagcatcga gttgaggctg gagcgtttca cgtggaagat tgttggattc gttcgcacca
     5761 gtggtgaagc gttttgcccc cgtgttcgct tcgaataagc cagatttcgc ctgttatcat
     5821 tgcgaagcat ctcgaaacag agcagatgcc gccgcgcttg cggctatgct ataaagttag
     5881 cggctactcc ttgagggacc tgtgccgttt ataaacttcc ccgcgcggga aattaactgc
     5941 aaacttgtct attatggccc cggcttaggc gggaagactg cgaatttgca gtggatatac
     6001 gagcacacag gctcgaatca aaaaggaaaa atggtttcgc tggccacgga aacggatcgc
     6061 acactgtttt tcgattttct tcccctggat ttaggaacgg tgcgcggatt tcgcacgcgc
     6121 tttcacctgt atacagtgcc cggacaggta ttctacgaag cgagccggcg attgatattg
     6181 aaaggcgcgg atggcgtggt gtttgtcgca gactcgcagg aagagcggct cgacgcgaat
     6241 tttgaaacca tcgacaacct gaaagaacat cttaaagacc ataatttgaa tttcgataca
     6301 attccttacg cgctacagct caacaagcgc gacctgccga acgcgatgcc ggtaccggag
     6361 ttgagaaaac agcttcaact caagggcgag ccggtgattg aagcggtggc tgttcagggc
     6421 cagggcgtgt tcgacacgct gaaagaagtg gcgaagctgg ttttgaacga gctcaagaaa
     6481 aattcctgaa aaaattctga agctcaggat tgcgcgggat tagcgcccga gaagctggcg
     6541 acggcctgct tttcatcatc caaggtggga ataatttcga tgaggcgcat gacttcgagg
     6601 acttcgcgcg cgtgtcccgc cggatgaagc attttgaaag agccgccgtg ctgcttcatc
     6661 atggtgtagc cgtggagcag gctgcagatg ccggagctgt cgagctgggt aactttggcc
     6721 agatttacaa gaatgtgccg ggaaccctgg cgcgcaagct catcgaggcg ctgcttgagg
     6781 gcatcagcgt caggcccaat caccaagcgg ccctcgagat cgatgatgct caccccggac
     6841 gattggcgaa cggtgatttt caaaggcatt cacttctcct atagcgctgc gtcgttttcg
     6901 tatccctcga atggatgcgt gaaattatac cgcagattcg atagaaggct gagggcgcac
     6961 gcgcaagtga taaacatgcg gtttgcttga cagcggctga atgcggaaat accgtccata
     7021 aagtgaaaca cctgagactg caccgatgga atgttacgcc gcgagaggct gcgcagattc
     7081 aactggaact gcgcgagcgg ctggagttga aggatcggct gccggtcgtc cactgtgttg
     7141 caggagctga tctggcgttt gatcttgcac ggaataaagc catcgcggga gtggtggttt
     7201 atcgtttccc ggagatggag gagcttgagc gcatctgggg cgagagcgag attcaatttc
     7261 cctatgtgcc cgggctgctg agttttcgcg aggcgccggc gctcttgaaa gtgttcgagc
     7321 gggctaagaa cactccggat ttaattttct gcgatgggca tggctacgcg catccccgaa
     7381 gatttgggat tacgtgtcat ttgggcgtgc tgttggatac gccgacgatt ggctgcgcga
     7441 agtcattgct tattggagag cacgatgcgc ttccgtcagg cgcgggtgcg tggacggagc
     7501 ttcgcgacaa agaagaagtg atcggagccg tgcttcgcac gcggtcgaac gttaaaccga
     7561 tctatgtgac gcaagggcac cgcatctcac tgcgtacggc gctgaagttt gtgaaggcgg
     7621 tgctcgatgg ctaccgaatc ccgcggccga cgagggacgc ggaccatttt gtcgcggcgg
     7681 ttaagaaaga aaacaaaaat gcggccgctg gcgtgtgaga gtagagggag gtagtgatcg
     7741 ggtgtaggca cggccaattc cgcttcgcgg ccgagttgag cttcgtatcc tatgagcggc
     7801 ttgtgatccc cggccagcgg aagctcgcgc actcttttct caagtgaaaa cggccaatcg
     7861 cagggctact gaacgtgccg tccccaagcg taggcgtgca ggttgtgcat taaattgccg
     7921 ctgctgagaa tgaggacatt ctcttcgcga agctgcgcga gccgcgcgcc gatctggtag
     7981 tgagaggacg gcggctgggt ttcgtcgatg ctgagctgca ccacgggaat atccgcacgc
     8041 gggtaaacgc ggcggagcac cgaccgggtg ccatgggtcg aggccccagc gctcatcacg
     8101 cctaaccggc aaaggtgcaa gaagctgctg gacgcgagca gcaaggtctg gatcgcccgg
     8161 cgcgggatac tgaatttcgt acagctcgcg cgggaagccg ccaaaaccat ggatggtccg
     8221 cgaagacttg cttaccgtga ctgccgcatc ttcaatgtac cagtgggcgt ctctgctcaa
     8281 tttattggat tcattctgtg cggagttgcc ctcaaaaaga accggccccc tggtgttccg
     8341 caagacgttt tccgttccgt aagcacttga tggtaaaagt agctgtcagg tcagtgggtt
     8401 tgaatcacga cggcttgcgc ctcgaagcaa tttcctcgaa acgctaagcg tcattcgcag
     8461 cgtggcttac ggaacttgaa tacttggatg gttcctcaat gtgtcacaac tttcgtaacg
     8521 agcaaagcag cgcggggctg ctgaatttga ccgagatg
//
LOCUS       DATNDT010000368        13817 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_373949, whole genome shotgun sequence.
ACCESSION   DATNDT010000368 DATNDT010000000
VERSION     DATNDT010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13817)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13817)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13817
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373949"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="VN788_11800"
     CDS             complement(<1..869)
                     /locus_tag="VN788_11800"
                     /inference="COORDINATES: protein motif:HMM:NF021619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
                     /protein_id="HXU21256.1"
                     /translation="MSGIAHRELSDEDRAKLLDVKAWDPILEMYGRTMKLAVALTDAE
                     GKLLGTCHNAQPIWSALHGSMIGKGGGCPFCLGPLSPCTAVADALRTGVPTITRDTAG
                     LAHVAVPVLLGDHPLGAILAGQVPSEYPESLSLQRASRTFGVSAQLLWSLSNQQRPVA
                     RATLRSAGELLFTLGQAFVQQRYGTLLEAHVADTNAQFRLLVDSVTDYALFTSDDRGR
                     VTSWNSGGERMLGYRDDEIIGEEFSRMFTSEDIQNGVPEKQLLKASQNRRTENEGWRV
                     RKNGTRFWADAIIT"
     gene            1247..2878
                     /locus_tag="VN788_11805"
     CDS             1247..2878
                     /locus_tag="VN788_11805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HXU21257.1"
                     /translation="MAVTDTNPLISENVNETAEIERARQLSARYRCPFLDLREQSIKA
                     DLVRSIPAELMFRYNFVPLELDDATLTIAIADPTHFQLIDELALHLGKRLQIRVAAPS
                     QINNLLKRTEQSQRVLDRASEGFALQVLPEDENSDENISMEKLTSEVGASPIVRLLDT
                     VIFTALERRASDIHVETSIDGVIVRYRIDGMLQCAMHPLAKEIHDTLLSRIKVMSDLD
                     IAERRVPQDGRFRVRYQNRFVDFRVSIMPSVYGEDAVLRVLDKESLSEKFRDLSLDVV
                     GLERDEVIRFRRFIREPYGMVLVTGPTGSGKTTTLYAAINEIKSEEDKIITIEDPVEY
                     QVRGTTQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDQETAQIAINSALTGHLVF
                     TTVHANNVADVIGRFINMGVEPYNFVSALNCIMAQRLIRMICHNCKKQVVCPPETLVE
                     SGLDLAEWAGVSMYEGAGCLECAGSGYYGRSAISELLNLTDRVREMIINRRPTSEIKR
                     AAKEDGMSSLRESGLRKVRAGLTTLREVNKVTFLD"
     gene            complement(2931..4394)
                     /locus_tag="VN788_11810"
     CDS             complement(2931..4394)
                     /locus_tag="VN788_11810"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HXU21258.1"
                     /translation="MSNAGSDTDVLIVGAGPVGLFLANECGRRGLRWRLMEARSSQSE
                     HSKALAIFPRTLEIFDMAGVVAPFLEKANRVTSVAIITHGRALAHMRFEPEETPYPFV
                     AMVPQDVTEKLLAEQLRHKGGNVEYETTFVSAEQQNGFVNVTADHKGEAIKLRALFVI
                     GCDGAHSAVRRGLKLPLEGGEYDDSFMLADIETNETLPADELQLCPSEFGPVAIFPMS
                     ATRRRIVATIEQPEADAPPLDLVRKVLEQRAPTGFEARALRWSSYFRIHHRHAPRLRV
                     GRIFIAGDAAHIHSPFGGQGMNTGLHDVWNLAWKLDLFLHGRGNERLLDSYSAERLPV
                     IKNVIETTDFLTKVMGTPNKFAQALRDTVIPMVSRFAPFQHAFVQRLSELGIAYSGSP
                     IIEGPGKRYFDDSMRGGEGIGSRFLVMVGEDSKPPVKEAAKQVCESLDGVVELRLAAH
                     AGVKLVRPDGYVAYSAHNHDSVAALGVLRSLVERQTS"
     gene            complement(4541..6844)
                     /locus_tag="VN788_11815"
     CDS             complement(4541..6844)
                     /locus_tag="VN788_11815"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXU21259.1"
                     /translation="MSDKALLSRREFVRDAGGLLIGFSLADATILPRVLAASPEDATK
                     STSPGQLDAWLRIEKDGTVRVFTGKVEIGMGVETGFAQIVAEELDVAPQRVQFVMGDT
                     SMTTDQGGVGGSTSIAFGAKPLRNVAATARYLLLQLAAQRLGAPLDQLQVRDGIVSVQ
                     GNAAKTVAYGDLAGAADLNDALNVSGRGFALNVEGIGKPKDPANYVVVGKPLPRVDIA
                     PKILGKWQYVTDVRVPGMLHGRVIRPGSAGAKLVSVDDASAKAIKGYVKTVVEGNFVG
                     IVAENEWAAIRGAQALKVTWSNTPSAFPAQTGLYKHMRTAAPKASKETLKKGDTAGAF
                     ANAAKKIEANYESPFQSHATMGPGCAVADVHLDGLTTVWSGGQKPHALQQGFAELLRV
                     PPEKVRVIWVEDAGSYGRPGFEDTAADAVLLSRAVGKPVRVQWSREDMTAWGTKGPAV
                     ICDMSAALDAHGEVSAFQFTSRAFSGGQVSFLPATAGNFLAGQLTGIPNTSGVDEFAQ
                     WGAECVAYNFPNVHADAHVVPAFLDVESPLRTTHVRDPEGPATTFATESFVDEMAAAA
                     GADPIEFRLKYLNDERLKAALTKAVEKFGWDRRPSPKHAGASEIATGRGVAIGLRGGT
                     RVATVAEVEVNRRSGTVHVKRLVCVHDCGLIVNPEALSGTVAANLIQSLSRGLKEEVK
                     FDGSRVTSVDWNTYPIARASDIPDQVDITLINHPEIPPSGAGEPASRPTAAAIANAIF
                     DATGARVRRAPLTPPRIKAALAAVHPA"
     gene            complement(6848..7321)
                     /locus_tag="VN788_11820"
     CDS             complement(6848..7321)
                     /locus_tag="VN788_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019485662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXU21260.1"
                     /translation="MASTSLKVNGKSHTVDVDADCPLLYVLRDNLALNNPRFGCGLGQ
                     CGACTVLMDNRAVRSCLLPVSRAAGKEIVTLEGLGSAENPDPVQQAFIDEQAFQCGYC
                     LNGWVLTAKALLHSNPHPTEAEMRRAFESLICRCGSHVGILDAVRKATGSKKEGA"
     gene            complement(7794..9224)
                     /locus_tag="VN788_11825"
     CDS             complement(7794..9224)
                     /locus_tag="VN788_11825"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S53 family peptidase"
                     /protein_id="HXU21261.1"
                     /translation="MNAKRLTLGVTSIGLLLFGLAGIAGAQGFDYIARPPYVQVSPNT
                     VIPNGLPYCGTTSGLTLICYAPNFIRTAYNFPSDLDGTGQTILIVDAYGSPTIQSDLI
                     LFDTIFNIPPPPSFAIFCPEGCPPYNANDKLHGEAGWSIETSLDVEYAHAMAPGANIV
                     LVVAASSSGDAINVAEAKAIAKYPGSIMSQSFGVGEFGIHGNNVEVMQAHKNYIAAQA
                     AGITVLASAGDDGAANAFMLGLKTIIGNVANASFPSSDPLVLAAGGTEGNPYQAIGTG
                     SFSCSANTICSTGLVTVRGPCDSVSLNDCTPVGYGGEQVWNEPDFAPGSTTGGAPSLL
                     FGIPSYQDGLGLASRTTPDVSYNAAINGGVLVVNSAVLGEPAFFIVGGTSAASPQWAA
                     IVALANQSAGQSLGFLNPAIYKLAQSAAYANDFHDIQTGNNVMAGTSVGFTAASGWDD
                     ASGWGTPNVANLVPDLVACATTATCP"
     gene            9509..10501
                     /locus_tag="VN788_11830"
     CDS             9509..10501
                     /locus_tag="VN788_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011482844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXU21262.1"
                     /translation="MGKLRLSMACSNYDRTRALFDGRVRVEGLELNCLDLPIEEIFFR
                     MLRHHEFDVAEISLSSYVLSLFGESPQLIAIPVFPSRFFRHSCIFIHSGSGIREPRDL
                     IGKRVGVPEFQMTAGVWIRGILSDDYKVPVGSVKYFRGGEEEPGRSEKIAVSLLPEIH
                     IESIPQTKTLSAMLESGEIDALYTARAPSTFGNGSGKVLRLFENYQAVEQEYFSRTKI
                     FPIMHTIVIRRDVYEKDRWVAQSLYRAFAQAQQMAYEELYDSGALHFMLPWLVRHAEE
                     TRALMGANFWPYGLERNTHTLEMFLRYSHEQGLAKRLLKPAELFAPESLESFKV"
     gene            complement(10498..10650)
                     /locus_tag="VN788_11835"
     CDS             complement(10498..10650)
                     /locus_tag="VN788_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21263.1"
                     /translation="MNIWAMMQPTCAIVASMSPRVSVHHGCEIAFPLDAREVSFGARA
                     KNSSAR"
     gene            10643..12079
                     /locus_tag="VN788_11840"
     CDS             10643..12079
                     /locus_tag="VN788_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21264.1"
                     /translation="MFIPVLLLVPVGVSLKFSLEHIVPGFAPGLLAGSAGLAWLGVRL
                     GKRENRKDVTAHAYGNNVPAILAYTLSIFLPVYLETHDAERAWEIGAAAVVWTGFIKL
                     IAAPFAGVFRRYIPVPASMTVFGAAMYSYLALILLQRVFDQPLVGIAALMIVGVAVFA
                     GVPITRYKLPPFLVAWLAPLGLGLLVGYVHPVWTGLSFVLPFVPIAGPLHAMVLAVPY
                     LSVIAPISIYHVLQDIASVEGGTSAEDNYDARSVVACDAVGTLIAGLAGSIVSPVIYA
                     LHPPYKALGARIGYAFWTPVIFLAVVMSGLISFMAGLFPWAILAAMIAYVSVGVGTAT
                     LRRVDRKYLSAVLLGFVLPAGAVVSAAVNSALPALQISTASASVQAALNRSIYWSSVQ
                     GLGNGFLFLVLVIASVVTEAIDRNFGRAAIWCVIASAFSWIGLMHSATLRWKAEPMYA
                     AGWLVAAAIVYSAKWWGHAAQENARSAG"
     gene            complement(12310..>13817)
                     /locus_tag="VN788_11845"
     CDS             complement(12310..>13817)
                     /locus_tag="VN788_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HXU21265.1"
                     /translation="LCAILLVASYFCYKALGSDLLPKMDEGGFIVDYIMPAGSSLQET
                     DRVLAHLEKMIGALPEVASVSRRTGLQLGLATVTEANTGDISVKLKTKRSKNVYDIMD
                     ELRDEFAQREPSVDVEFVQELQDMIGDLSNAPEPVRIELFSSDADLLQQWAPKVADAI
                     SAIQVSGKKPVVDVLNGIENTISGPAVTFRVNPSVAQRAGFTPEEVSTDAEAILEGAQ
                     APTPVVANDRAYTIRVRFPDPNRASLDAMSNTLLVSSSGQTATLGSLSTIAESPGQTE
                     ILRDNLQREVAVTARFEGLDLGSGIRAVQKTVGGLHLPSSIRVEYGGLYKEQQKSFHD
                     LVVVLVLALALVFIVLLFEFGGFAGPLAILTSALLSTSGVLFALWITRVTFNISSFMG
                     LIMVIGIVAKNGILLLDADQKFRAVGFSPEEAMIQAGRRRLRPIVMTALAAMSGMLPL
                     ALAWGAGSQMLQPLAIAVIGGILISMVLSLIITPAMYFNLSKGHRQPTNTG"
BASE COUNT         2816 a         4049 c         3920 g         3032 t
ORIGIN      
        1 gtgatgatgg cgtcagccca aaaccgcgtt ccgttcttgc gaactcgcca gccttcattt
       61 tccgtgcgcc tgttttgcga agccttgagc agttgtttct ccggaacgcc attctgaatg
      121 tcctccgagg tgaacatccg cgaaaattcc tcgccgatga tttcatcgtc gcgatagccc
      181 agcatccgct cgcctccgct gttccaactg gtcacgcgtc cgcggtcgtc gctggtaaaa
      241 agcgcgtagt ctgtcacgct gtcaaccagc aatcgaaact gagcgttggt atctgccacg
      301 tgcgcctcga gcagggtgcc gtaccgttgt tgcacgaacg cctgaccaag cgtgaacaag
      361 agttcccccg ccgatcgcag ggtcgcccgc gccacaggac gttgctggtt cgacaagctc
      421 caaagcaatt gcgccgaaac tccaaacgtt ctcgaggctc gctgcagaga aagtgactcg
      481 ggatattcgc taggaacttg tccggcgaga atggcgccca gcggatggtc gcccaataac
      541 acaggaacgg ccacgtgggc caggccggct gtgtcgcgcg tgatcgtggg cacccccgtc
      601 cgcaacgcat cggccaccgc agtgcaaggt gagagcggac ccaggcagaa gggacagcca
      661 cccccttttc caatcattga gccatgaagg gcgctccaga ttggctgggc attgtggcat
      721 gttcccaaca gctttccttc ggcatcggtg agcgctaccg ctagtttcat tgtccggccg
      781 tacatctcca ggatcggatc ccacgccttc acgtcgagta gcttggcgcg gtcctcatcc
      841 gacaattccc ggtgagcaat tccgctcacg gctccgccgc cgtcgggaga tccctcttcc
      901 ttcgccctcg gtaaacgccc cttcagatca atcgttgttg gttcgggcgg aatggtacat
      961 tcctttaaat cgttccattg ctataagaac gatcggctcg cattattttt actccagacc
     1021 ctgtcgagag aaaccctgct gccgatgtac ttcaagatag gttctcatta gttactctgt
     1081 ttccatttgg aacacgggct taggatgtga cggaagccac ccaacaattt tcagaattga
     1141 gatttgtgtc cagaacagaa ctttattttt ttcgcttccg tcgcactatt gggccctata
     1201 catcgaagcc acggcaaaaa aatcaagaat ctcaggagta ttccgaatgg cagtcaccga
     1261 cactaacccg ttaatctctg aaaatgtgaa tgagaccgcc gaaattgaac gcgctcgcca
     1321 actctccgcc cgttatcgct gcccctttct cgaccttcgg gagcagtcca tcaaggcgga
     1381 cctcgtccgc agcattccgg cagaattaat gttccgctac aactttgtgc cactcgagct
     1441 tgacgatgcc actcttacta tcgctattgc cgatcccact cacttccaac tgattgacga
     1501 actggccctg cacctcggta agcgccttca aatcagagtc gccgctccgt cgcagatcaa
     1561 taacctcctg aagcgtacgg aacaatcgca gcgggttctc gatcgcgcct cggaaggttt
     1621 tgcgctccag gttctccccg aggacgaaaa ctctgacgaa aatatttcaa tggagaagct
     1681 caccagcgag gtcggcgcga gccccatcgt ccggcttctc gacaccgtta tcttcacggc
     1741 tctcgagcgg cgggccagcg atattcatgt ggagaccagc atcgatgggg taatcgtgag
     1801 gtaccgaatt gacggcatgt tgcaatgcgc catgcatccg ctcgcgaagg aaatccatga
     1861 cacacttctc tcgcgaatca aggtgatgag tgacctggat atcgccgaac ggcgcgtccc
     1921 acaggatgga cgtttccgtg tgcgttacca gaaccgcttc gtcgatttcc gcgtttcgat
     1981 catgccttcc gtttacggcg aagacgccgt gcttcgcgtt ctcgataagg aaagcctcag
     2041 cgagaagttt cgcgacctaa gcctcgacgt ggtcggtctg gaacgcgatg aggtgattcg
     2101 cttccgccgt ttcatccgcg agccttacgg catggtactg gttactggcc ccacgggctc
     2161 cggcaaaaca actacgctct acgccgccat caatgagatc aaaagcgaag aggacaagat
     2221 catcaccatc gaggatcccg tcgagtatca ggtgcgcggt actacccaga ttccggtgaa
     2281 cgagaaaaag ggcctgacct tcgcgcgtgg ccttcgctca attttgcgcc atgatcccga
     2341 taagattatg gtcggcgaga tacgcgatca ggaaaccgct cagatcgcca tcaattcggc
     2401 gctaacgggc cacctcgttt ttaccacggt ccacgccaac aacgtcgccg acgtgatcgg
     2461 gcgcttcatc aacatgggag tcgagccgta taattttgtt tctgcgctca actgcatcat
     2521 ggcccagcgc ctcattcgta tgatctgcca caactgcaaa aaacaggttg tttgcccccc
     2581 ggagacgttg gtcgaatccg gcctggacct cgcggaatgg gctggcgtct cgatgtatga
     2641 aggggctgga tgtctcgaat gcgcgggaag cggctactac gggcgctccg ccattagcga
     2701 actcttaaat cttaccgatc gtgtccgcga gatgataatc aaccgtcgtc caacttcgga
     2761 aattaagcga gccgccaaag aagacggcat gtcgtctctt cgagagtcgg gccttaggaa
     2821 ggtgcgcgca ggcctgacca ccctgcgcga ggtgaataaa gtaacgttcc tcgactgagg
     2881 cgctcgcgtt gtccatgccg ctttcttcgc cgttaccagc aggactgttc ctagcttgtt
     2941 tggcgctcaa ccaacgagcg caacaccccc agggccgcga cgctatcgtg gttgtgcgcg
     3001 gaatatgcga catagccatc gggccggaca agctttacgc ccgcgtgcgc tgccagccgc
     3061 aattcaacga ctccatctag cgattcgcag acctgcttcg ctgcttcctt gaccgggggt
     3121 ttgctgtcct cgccgaccat caccaggaag cggctgccga taccctcgcc gccgcgcatc
     3181 gaatcatcga agtatcgctt cccgggtccc tcgataatcg ggcttccgct ataggcaatg
     3241 ccaagttcag aaagcctctg cacgaaagcg tgctggaacg gcgcgaagcg cgacaccatg
     3301 ggtatcacag tgtcgcgcag ggcttgagca aacttgttcg gagtccccat taccttggtc
     3361 aggaaatctg ttgtctcgat cacgtttttg attactggaa ggcgttcggc gctatagctg
     3421 tcgagtaacc gttcattgcc acggccgtgc aggaagagat cgagcttcca ggccagattc
     3481 caaacatcat gcagtccagt attcatcccc tgcccgccga atgggctgtg aatgtgagcc
     3541 gcatctccgg ctataaagat gcgcccgacg cgaagccgcg gtgcgtgccg atgatggatc
     3601 cgaaaatagc tgctccagcg aagggcgcgc gcctcgaacc cggtcggcgc gcgttgctca
     3661 agtactttcc gaaccaggtc cagcggcgga gcgtctgctt ccggctgctc gatcgtcgcc
     3721 acgatacgcc ggcgcgtggc gctcatcgga aaaatcgcaa ccggcccgaa ttcgctgggg
     3781 caaagctgca gttcgtccgc cggaagagtt tcattcgtct cgatatccgc caacatgaac
     3841 gagtcatcgt attctccgcc ctcaagcggc agcttcagcc cccgccggac ggcactgtga
     3901 gcgccgtcgc agccgatgac aaacaaagct ctgagcttta tcgcttcgcc cttgtgatcc
     3961 gcggttacat tcacaaaacc gttctgctgt tccgccgaaa cgaatgtggt ctcgtactcg
     4021 acgtttccgc ccttatgccg caattgttcg gcgagtagtt tctcggtcac atcctgggga
     4081 accatggcaa cgaaaggata gggggtctcc tccggctcga accgcatgtg cgcgagcgcg
     4141 cggccgtgtg tgattatcgc gaccgacgtg acacggttcg ccttttcgag aaacggagcc
     4201 acgacgcctg ccatatcgaa aatttcaagc gtccggggaa atatggccag cgctttggag
     4261 tgttccgatt gcgacgagcg cgcctccatg agccgccatc ggaggcctcg gcgaccacat
     4321 tcattcgcca gaaataatcc aacaggcccg gcaccaacga tcaaaacgtc tgtgtcactt
     4381 ccggcatttg acattgatcc atcctcttta actgaaagcc gccaggaata ttttcatgat
     4441 gcatcccttc actactagac tcttcggcag ttgaaacgct tcgcaacgcg catcgtcatc
     4501 tcgcgcgaaa cgatcgcatc tcaaattcgg cggttatcgg tcaggcgggg tgaaccgcgg
     4561 cgagagcggc tttgattctt ggcggcgtca gaggtgcgcg gcgcacccgt gcaccggttg
     4621 catcgaagat tgcgttagcg atcgcggcag cggtgggccg gctggccggc tcgccggcgc
     4681 cgctcggggg aatctccgga tggttgatca acgtgatgtc cacttgatct ggaatatccg
     4741 acgcgcgtgc gatagggtat gtgttccaat ccacactggt cacgcgagag ccgtcgaact
     4801 ttacttcctc ttttagaccg cggctcagcg attgaatcag attcgcggca accgtcccgg
     4861 agagtgcttc ggggttgacg atcaatccgc aatcgtggac gcacacgaga cgcttcacgt
     4921 gaaccgttcc gctgcgccga ttcacttcca cttccgccac tgtggccacg cgagttcctc
     4981 cgcgcaagcc tatggccacg ccgcgacccg tcgcaatttc cgaagcgccg gcgtgcttcg
     5041 gagaaggccg gcggtcccag ccgaattttt cgactgcttt cgtcagcgcg gctttcaggc
     5101 gttcgtcgtt cagatacttg aggcgaaatt cgatgggatc ggcacccgcc gccgcggcca
     5161 tttcgtccac gaaagattca gttgcaaacg tggtggcagg gccttcggga tcgcgcacgt
     5221 gagtggtgcg cagtggagac tcgacatcca aaaaggcggg aaccacatgc gcgtccgcgt
     5281 gaacatttgg aaaattgtag gccacgcatt ccgcgcccca ctgcgcaaac tcgtcaacgc
     5341 cactggtgtt tggaattccc gtgagttggc cggccaaaaa attaccggcc gtcgccggca
     5401 agaaactcac ctggccgccg gagaatgcgc gcgatgtgaa ttgaaaggcg ctcacctcgc
     5461 cgtgcgcatc gagagcggcc gacatgtcac agatcacggc gggacccttg gttccccacg
     5521 ccgtcatgtc ctcgcgcgac cattgcacgc gaaccggctt cccaacagcc cgcgaaagca
     5581 gcaccgcatc cgccgccgtg tcctcaaaac cgggccggcc gtatgaaccg gcatcttcaa
     5641 cccaaataac gcgcactttt tccggaggta cgcgcaacag ttccgcaaat ccttgctgca
     5701 aagcgtgcgg cttttgcccg ccggaccaca cggtggtgag accgtccaag tggacgtccg
     5761 caacagcgca accgggaccc atggtggcgt gtgactgaaa cggcgattcg taattggctt
     5821 cgattttttt ggccgcgttt gcgaaagcgc ctgcggtgtc cccctttttc agagtttcct
     5881 tgctggcctt gggcgcagcg gttcgcatgt gtttgtacag acccgtttgc gcaggaaaag
     5941 ccgatggcgt gttgctccag gtaactttca gcgcctgggc gccgcggatg gcggcccatt
     6001 cgttctccgc cacaatcccg acaaaatttc cttcaactac cgtcttcacg tagcccttga
     6061 tcgcttttgc ggatgcatca tccacgctca ccaacttcgc gccggcgctc cctggacgaa
     6121 tcacgcggcc gtgcagcatt ccgggaacgc gcacatcggt gacgtattgc cactttccca
     6181 aaatcttcgg agcgatgtcc acccgcggaa gcggtttacc aacgacaacg tagtttgcgg
     6241 gatctttggg tttgccaatt ccttccacgt tcaacgcaaa tccgcggcca gaaacgttga
     6301 gcgcatcgtt taagtctgca gctccggcca aatctccata tgccacagtc tttgcggcgt
     6361 ttccctggac gctgacgatc ccatcgcgta cttgaagttg atcgagcggc gcgccgagac
     6421 gctgcgcggc aagctgcaag agcaaatatc gcgcggtggc ggcaacgttt cgcagcggct
     6481 tggcgccaaa cgcgatggat gtgctgccgc cgacgccgcc ctggtcggtg gtcatggacg
     6541 tgtcgcccat tacgaactgg acgcgttgtg gagcaacgtc gagttcctcg gccacaatct
     6601 gcgcgaagcc cgtttcgact cccattccga tttccacttt gccggtgaag acgcgaacgg
     6661 ttccatcttt ttcgattcgc agccaggcat cgagctgacc cggagaagtg ctctttgtcg
     6721 catcttcggg tgacgcggct aggacgcggg ggagaatagt tgcgtcggcc aggctgaatc
     6781 cgatcagcag gccgccggca tcgcggacaa attctcttct cgatagaagg gctttatcgg
     6841 acataaatta tgcgccctcc ttcttggaac cggttgcctt gcggaccgca tccagaattc
     6901 ccacgtggct cccacaccgg caaatcaaac tttcaaacgc ccgccgcatt tcggcttctg
     6961 tcggatgagg attgctgtgt aagagcgctt ttgctgttag cacccagccg ttgaggcagt
     7021 agccgcactg aaaagcctgc tcgtcgatga atgcttgctg aactggatcg ggattctctg
     7081 cagagcctaa tccttcgagg gtgacgatct cttttcctgc tgcgcgcgag accggcaaga
     7141 ggcaggagcg caccgcgcga ttatccatca gcaccgtgca cgcgccgcac tgtcccaagc
     7201 cgcaaccgaa gcgcggatta ttcagtgcga gattgtctcg aaggacgtag agcagggggc
     7261 agtcagcatc cacgtccacc gtgtgcgact tgccatttac cttgagtgat gtgctagcca
     7321 tttatgatct cgattcctga agctgccttc gcgggctagt ttactcctga caggcagtct
     7381 gaaaaaagcg gaatggaatt ttttgccggg aagagtccta aaaccggggg aattgtcctc
     7441 ttgattttgt gacgggtagc cctgacgcct gtcgtttgtt ttcaatgggt tagagcagat
     7501 tctactcggc tcatgtgtcg aatagaattg caagccaatc gcgttggact gccgactcaa
     7561 taacgccgac gagctgccct tcggcttcgt tcgtgatgag ccgatgctac gaccgcctcc
     7621 gctttctcca aacactgcgg ttctcctttc ccactcaagt tagcactgca agaaagcgtt
     7681 gcaaggacca gctgctgtgg actttctgag gacaagatcg cagggctacc gagagacgcc
     7741 tgtaccatcg ctggccgcgc ctgcctattc gcctctgtgt ggctcattcc tgactaggga
     7801 cacgtcgcag ttgtcgcgca tgcgaccaaa tctgggacga ggtttgcgac attgggcgtg
     7861 ccccaacccg aggcgtcatc ccagccacta gccgccgtga agccgactga ggtgccggcc
     7921 atcacattgt tgccagtctg gatgtcgtga aagtcgttgg cataagcagc tgattgcgcc
     7981 agcttgtaga tcgccgggtt caggaatccg agggactggc cggccgactg attggcgagg
     8041 gcgacaatgg cagcccattg gggtgaggct gcgctggtac ctcctacgat gaaaaacgcc
     8101 ggctcgccaa gcacggcact attgaccacc agcacaccac cattaatcgc agcgttgtaa
     8161 gaaacgtccg gcgtcgtcct tgatgccagc cccagtccgt cctgatacga aggaatccca
     8221 aaaagtaaac tcggtgcgcc acccgttgtt gagccggggg caaaatccgg ctcgttccac
     8281 acctgctctc ctccgtagcc aactggcgtg caatcgttca acgacactga atcgcaggga
     8341 ccacggaccg tcacaaggcc agtcgagcaa atagtgttcg ccgagcaact gaaactgcct
     8401 gtgccaatgg cctgataagg attcccctcc gtgccgccgg ccgccaaaac cagaggatca
     8461 gaggagggga aactggcgtt cgcgacatta ccaatgattg tcttgagccc aagcatgaag
     8521 gcgtttgccg cgccatcatc tcccgccgac gctagtacgg tgattccagc tgcctgcgcc
     8581 gcaatatagt tcttgtgggc ctgcatgacc tccacattgt tgccgtggat tccaaattct
     8641 ccaacgccaa agctctgcga catgatgctg ccgggatact ttgcaattgc cttcgcttcg
     8701 gcgacattaa tcgcgtcccc tgagctgctt gcggccacca ccagtacgat attcgcgccc
     8761 ggcgccattg catgcgcgta ctccacatcc aggctggtct cgatgctcca gccagcctcg
     8821 ccatgcagtt tgtcgttggc attgtagggc gggcatcctt ctggacagaa tatcgcgaaa
     8881 gatggcgggg gcgggatgtt aaagatggtg tcgaagagga taaggtcgga ctgaatcgtg
     8941 ggactgccgt aggcgtccac aatcaaaatt gtctgacctg ttccgtccag gtccgatggg
     9001 aagttgtacg ccgtcctgat gaagttcggc gcgtagcaga tcagcgtcag ccctgatgtg
     9061 gtgccgcagt agggcagccc gttgggaatc acagtgttcg ggcttacctg aacgtaggga
     9121 gggcgagcga tatagtcaaa gccctgcgcg ccggcgatgc cagccagccc aaacagaagc
     9181 agcccaatcg acgtcactcc gagtgtcagt ctttttgcat tcatcggaat ctcctcgcat
     9241 tcgagaacgg gtcttagtag ggccgtttcc ggctggggcg gggacggacg aaccagcaca
     9301 atagccgcaa gagccggcgt gggtcaagaa ttattgcgtt aatattcggt taatagcggg
     9361 ctggacttgt ggtggctgct cccacaacta tgctttgccc ttcgcataga gtcagttctt
     9421 gagccatctg ggctttgagt atactgtggg cgccaaaccg gcgacggcgc gggggcatac
     9481 ctcttcgacg cgagcgaggg aattgggcgt gggtaagctg cggctttcga tggcttgttc
     9541 gaattacgac cggacgcgcg cgctatttga cgggcgcgtg cgcgttgagg gactcgagct
     9601 caactgcctg gacctgccta tcgaggaaat tttctttcgg atgttgcggc atcacgagtt
     9661 tgacgttgcg gagatatcgc tatcgtcgta tgtgctgtcg ctttttggcg aaagtccgca
     9721 gctgattgcg attccggtat ttccatcacg gttcttcagg cactcctgca ttttcattca
     9781 cagcggcagc ggaatccggg agccgaggga cctgatcggc aagcgtgtgg gcgtgccgga
     9841 attccagatg acggcgggcg tgtggattcg cggcattctg agcgacgact acaaagttcc
     9901 tgttggcagt gttaagtatt tccgtggagg cgaagaggag ccgggccggt ccgaaaaaat
     9961 tgcggtgtcg ctactgccgg aaattcacat cgaatccatc ccgcagacaa agacgctttc
    10021 cgcgatgctt gaatcgggcg aaattgatgc gctctatacg gcgcgcgccc cttcgacgtt
    10081 tggcaatggt tccggcaagg tactgcggct tttcgaaaat tatcaggcgg tggaacagga
    10141 gtatttttcg cggaccaaaa tattccccat catgcacacc atcgtaattc gccgcgatgt
    10201 ttacgaaaaa gaccggtggg tggcgcagtc actttataga gccttcgcgc aggcgcaaca
    10261 aatggcgtac gaggaactgt acgattcggg cgccctgcac ttcatgcttc cctggctggt
    10321 gcggcatgcc gaggagactc gcgcgctaat gggcgcaaac ttttggccgt acggactgga
    10381 acgaaacacc cacacgctgg aaatgtttct gcgttactcg cacgagcaag gattggcaaa
    10441 gcgtctattg aagccggcgg agctatttgc tccggaatcg ctggaatcgt tcaaagtcta
    10501 acgcgccgaa ctgtttttcg ctcgcgcgcc gaaggagact tccctggcat ccagcggaaa
    10561 cgcgatttcg cagccgtggt ggacgctgac tcgcggggac attgacgcga caatcgcgca
    10621 agtcggctgc atcatcgccc agatgttcat tccggtgctg ctcctcgtgc cggtcggtgt
    10681 ctctctaaaa ttcagcctcg agcacattgt gccgggcttc gcgccagggt tgcttgcagg
    10741 gtccgcgggc ctcgcatggc tcggagttcg gctgggcaag cgcgaaaacc gcaaggatgt
    10801 gacggctcac gcttacggaa acaacgttcc ggcaatcctc gcgtacacgc tttcgatttt
    10861 cctgccggta tatctagaaa cgcacgacgc ggagcgcgcg tgggaaattg gcgcggctgc
    10921 tgtggtctgg acggggttca tcaagctgat cgcggcgccg ttcgccgggg ttttccggcg
    10981 ctacattccg gtgcccgctt cgatgacagt gtttggcgcg gcgatgtaca gctaccttgc
    11041 gcttatcctg ttgcagcgcg tgttcgatca gccactggtg ggaatcgctg cgctgatgat
    11101 tgtgggcgtg gcggtgtttg caggcgtgcc gatcactcgc tataagctgc ctccattttt
    11161 ggtggcgtgg ctggcgccgc tgggtctcgg attgctggtg ggctacgtgc atccggtgtg
    11221 gacgggatta tcgtttgtgt tgccgttcgt tccaatcgcg ggacccctgc acgccatggt
    11281 gctggcagtg ccgtatcttt ccgtgatcgc tcccatttcc atttaccatg tgctgcaaga
    11341 tatcgcgtct gtcgaaggag gcacatctgc ggaagacaat tacgatgcgc gcagcgtggt
    11401 ggcctgcgat gcggttggca cgctgattgc cgggctggcc ggcagcatcg tgtcgccagt
    11461 gatttacgcg ctgcatccgc cttacaaggc gctgggagca cgcatcggct atgcgttctg
    11521 gactccggtg atttttctcg cggtggtgat gagcggtttg atctcgttca tggcgggact
    11581 atttccatgg gcgattctgg cagcgatgat cgcttatgta tcggtcggcg tggggacggc
    11641 tacgctgcgg cgagtggatc gaaaatatct gagcgcggtg cttttgggat tcgtgctgcc
    11701 cgcgggcgcg gtggtatccg cggcggtaaa ttccgctttg cctgcgctgc agatttctac
    11761 agccagcgcc agtgtgcagg cggcattgaa ccggtcaatc tactggtcgt ccgtgcaagg
    11821 tttgggcaac ggatttcttt ttctggtgct ggtgattgcg tcggtggtaa ccgaggcgat
    11881 tgaccgcaat ttcgggcgag cggctatttg gtgtgtgatt gcgtcagcat tttcctggat
    11941 tggcctgatg cactcggcaa ctttgcgctg gaaggcggaa ccgatgtacg ccgcaggatg
    12001 gctggttgcc gcagcaattg tttactccgc taaatggtgg gggcacgcag cgcaggaaaa
    12061 cgctcgaagt gcaggctgag cgaaaagcgc ggcgcgatcg gggagtgctt acaaaaattg
    12121 agaatcaacc gtccctccgg tacttctgaa ttttgctttt cctaacccgg cactccgctt
    12181 cgttccgtgc cgggctaatt acaatcgccg ctccgcggct taaaaaaagg caaattccta
    12241 ggcggtcgca cctgcaagcc tgcgctactt cgcaagctct cgcgacacct tgatgagacc
    12301 ggtttgagat tagccggtgt ttgtgggctg ccggtggcct ttcgacaaat tgaaatacat
    12361 cgccggtgtg ataatcaaag acagcaccat ggagatcaga attcctccga tcactgcaat
    12421 cgcaaggggc tgcagcattt gcgatcccgc tccccaggca agcgcaagcg gaagcatgcc
    12481 ggacatggcg gccagcgccg tcatcacgat gggacgcaag cgccttcttc cggcctgaat
    12541 catggcctcc tcgggcgaaa atccgacggc gcggaatttc tggtcggcat cgagaagcaa
    12601 aattccgttt tttgccacga tgccgatcac catgatcaat cccataaatg aagagatgtt
    12661 gaacgtaact ctggtaatcc aaagagcgaa aaggacgccg gaagtggaaa gcagcgcgga
    12721 cgtaagaatc gccaacggcc ccgcaaaacc tccgaactca aacagcagca cgatgaaaac
    12781 cagagcaagc gcaagcacca gcacgaccac cagatcgtga aaagatttct gctgctcctt
    12841 gtacaggcct ccgtattcca cgcggatcga agacggcagg tgcagtccgc caaccgtctt
    12901 ttgcaccgcg cggatgccac tgccaagatc caacccttcg aaccgcgccg taacagcaac
    12961 ttcgcgctgc aaattgtcgc gaaggatttc cgtctgcccc ggcgattccg caatcgtcga
    13021 gagcgaaccc agagtggcgg tctggccgct agagctaacc agcaacgtgt tgctcatcgc
    13081 gtcgagcgaa gcccgattcg ggtcgggaaa gcgcacacgt atggtgtacg cgcgatcgtt
    13141 ggccaccacc ggagtcggcg cctgagctcc ctccagaatc gcttccgcgt cggtggaaac
    13201 ttcttcggga gtaaaaccgg cgcgctgggc gacgctcggg ttcaccctaa aagtcaccgc
    13261 cggtccactg atcgtattct cgatgccgtt cagcacgtcg acgacgggct tctttccgct
    13321 cacctgaatc gcgctgatcg catccgccac tttgggcgcc cactgctgca gcaagtccgc
    13381 atcggacgag aagagttcaa tcctgacggg ttccggcgcg ttcgaaagat caccgatcat
    13441 gtcctgaagc tcctggacga attcgacatc tacggagggt tcccgttggg cgaactcgtc
    13501 ccgtagctcg tccatgatgt catacacgtt tttgctccgc ttggtcttga gctttaccga
    13561 aatgtctccg gtgttggctt ccgtgacggt cgcaagcccc aattgcagcc cggtgcgtct
    13621 ggatacgcta gcgacttcgg gaagcgcgcc aatcatcttc tcgaggtgcg ctaagactcg
    13681 atcggtctct tgtagtgaac tgccggcggg cattatgtag tcgacaatga atcctccctc
    13741 gtccatcttg ggcagcaagt ccgatccgag cgccttgtag caaaagtagg aggccaccag
    13801 cagaatcgcg cacaaac
//
LOCUS       DATNDT010000369         7875 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1529037, whole genome shotgun sequence.
ACCESSION   DATNDT010000369 DATNDT010000000
VERSION     DATNDT010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7875
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1529037"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VN788_11850"
     CDS             complement(<1..796)
                     /locus_tag="VN788_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21266.1"
                     /translation="MKSKVSFISILIAATIAVVPRATAQDGSIEFVAHATPSGGLEEP
                     VRGFPFYLLRKSFEEIGKEADADDPKPDMRTFIDKLDSSSYSPQLKAWMKKNQFVTLS
                     GEDFIRKLHPGDVMDIPEFFSAYKDRNAGDQSADFPKSKVKASDKVKNPARYEKATAK
                     YKEMIRRYIEQHPQSVDGIDLNLAEIDPGPQWNALLGKRTPEIHRRALDLAQSKYLVA
                     RAESNLQGQGFLRDVPPGSYWISTLDVAADVGDARPRWDVAVSVLPG"
     gene            complement(928..2118)
                     /locus_tag="VN788_11855"
     CDS             complement(928..2118)
                     /locus_tag="VN788_11855"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXU21267.1"
                     /translation="MRTFDLSTRRDKGDRSTSGSTEPGLRLGRLAAPVHESSLKSLYS
                     NLRDFLTERPAKVRPGTPTAFDMPKFGTGLGANLKEFFHPAPRGRVKSALLVNWKEEP
                     TLWQNLRDWIAPRKLPPLQTTSQPIPVPEIWSKNTQFNRVQAVSILVHAVAIALVVLI
                     PLLLPGLISPPTTKAYNPEPPVDVSVSPYLAKLKPSAKAAGGGGGAHNTEPAAKGKLP
                     KFSWTQFSAPMAHPVEHPQIAMTPTVVGNPDIKLPNQNLPNWGDPLGKSLSDSMGQGH
                     GSGVGNGNGAGVGPGEGWNTGGGAPNAGTGGYGVPACLYCPRADYSDEAMKVKVQGVV
                     ELIAIVTADGRVTDIHVAKGLGLGLDEKAIQAVRTWRLTPARGPDGRPAAVRQIIEVA
                     FQLF"
     gene            2397..5174
                     /gene="ileS"
                     /locus_tag="VN788_11860"
     CDS             2397..5174
                     /gene="ileS"
                     /locus_tag="VN788_11860"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HXU21268.1"
                     /translation="MKARLPEREPDQLLAWEEMGLYARILEQRAGAPLFVLHDGPPYP
                     TGEIHLGTGLNKIVKDMIVKSKTMAGFRSPYVPGWDCHGLPIETQVEKELGGKTTKVS
                     AVEFRRLCREFAARYVEAHKRDFKRLGVFGRWDRPYLTMDPGIEACIASAFIDFFEKG
                     YVYRGLKPVYWCIRDRTALAEAEVEYEDHTSPSIWVKFPIVPGPKTPALGNDVNALIW
                     TTTPWTLPANRALAFHPDFEYTVAETTAGKLLVAKARIAAIFDELKLEIGKVHGSWKG
                     SELEGIQFQHPFLDLRVPAVLADHVTLDQGSGIVHTAPGHGAEDFRTGQKYGLEAYAP
                     QDDDGRFVEGLPEYKGKTVWEANPIIIDLLMNRGMLVGGRQFTHSYPHCWRCHNPVIF
                     RATEQWFVGLDHDGLRQNALTEIAKVKWKPSWGEARIREMVAERPDWCISRQRFWGVP
                     LIVFTCESCGEQLKDFDALRHVLPFFEREGADAWYAHTTEELLPPETKCSCGSAKWRK
                     ENDILDVWFDSGSTHLAVLSEKDGTWPADVYMEGPDQYRGWFQSSLLIGVGTRGRAPY
                     RQVVTHGWTLDEKGAPMSKSLGNAMYPKEICDKWGADLLRVWVSSQDFTQDVRLSDAM
                     MKQLAEAYRKIRNTFRFALSNLFDFEPGRDIVHDAEMWEMDAWMLRRTGALIRQCNGW
                     YKEFEFHRVYHAIQDFCTVDLSAFYFDVLKDRLYTFAPNNRGRRSAQTAVYHIARALV
                     RLVAPILVFTSEEIWKYLPRGASAQPSVHMASFPSPEPFEKAFDDARAKEWDRLLVVR
                     EEVMRALESLRAAKTISAGLEARVTLTAKGDLAALLHSHVASLPAFFIVSQVEIATDN
                     SDGGKPAAGVDGGLRTHVERAHGKKCERCWNYSTHVGENADYPNWCERCVAAMDEIER
                     DGGSVAGSAAS"
     gene            5174..5680
                     /gene="lspA"
                     /locus_tag="VN788_11865"
     CDS             5174..5680
                     /gene="lspA"
                     /locus_tag="VN788_11865"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007294516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HXU21269.1"
                     /translation="MRSVSRPGWVLTVLAVAVLILDQASKYAVQNLTPLGSSRVLIPG
                     VLNLVHSTNPGVAFGLLADSQMPWRAPLLIVFSVAVIGLITWLLVTDRAGDRLGQWGM
                     ALILGGAAGNVLDRIVRHSVTDFIDFYVGSHHWYTFNVADSAIVIGAALVLLELLRDW
                     RHPSQERA"
     gene            5683..6510
                     /gene="lgt"
                     /locus_tag="VN788_11870"
     CDS             5683..6510
                     /gene="lgt"
                     /locus_tag="VN788_11870"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HXU21270.1"
                     /translation="MHPVLFQIGSITIYTYGVLAAAGFLVGLWLAYRQAPRAGLNPHS
                     VWNLGVYGILLALLLSKVWLIFSSWDYYVAHPGQIFSMATFQSAGTFYGGILGGILWV
                     VLYTHFQKMPLLAVLDVVAAPVALGHAIGRIGCFVAGCCYGKPTSLPWGVTFTSPVAE
                     KIAGTPLHISLHPTQLYEAGAEFLNVLLLVWLGARQRFAGQLIGAYFVLYGIERGVIE
                     FFRGDPGRTMMFHDAVSLMQIVSAVLIIAGGSLWWRGLHRIDSTVPAPTARAMAGRS"
     gene            6542..7552
                     /locus_tag="VN788_11875"
     CDS             6542..7552
                     /locus_tag="VN788_11875"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HXU21271.1"
                     /translation="MSFSLSVQHLEFTVPPDCSGQRLDRFLVSQLSELSRSRIQSLMD
                     QGRVLVDGVARKASYHVASGETLGVEIPPTPAAGVEAEPIPLHILYEDEDVAVINKPA
                     GMIVHPGAGADAGTLVAALLNRFGGSAGLSTIGGPLRPGIVHRLDKDTSGAILIARTD
                     EAHRKLVEDFRERRIDKTYVALLHGKIKGDSGSVELAIARDLRRRSRMTARRRVGREA
                     RTDWRVLLRLKGHTFIEASLYTGRTHQIRVHFSALGHPVVGDTLYGAPRQERAASALL
                     PPLNRNFLHAARVGFVHPRTGRRIEVRAPLPPQLVDYLAFLARSVQVDPHSVDAALRE
                     FL"
     gene            7575..>7875
                     /locus_tag="VN788_11880"
     CDS             7575..>7875
                     /locus_tag="VN788_11880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21272.1"
                     /translation="MNRPGSLRTAVFVLLILFAVCGICARAQEAPSKPVAPATSAPQQ
                     APATPNPQAAAPPQQPPIVSTTGLVHLVVTVVDRHHDFITDLDKTDFKVLDNGSPQ"
BASE COUNT         1546 a         2371 c         2227 g         1731 t
ORIGIN      
        1 gtcccggcag cacgcttaca gccacgtccc atcgcggccg agcatcgccg acgtccgcgg
       61 cgacatcgag tgtgctgatc caatagcttc ctggcgggac gtcgcgcaaa aatccttgcc
      121 cttgaaggtt cgattccgcg cgcgcgacca aatatttaga ctgagccaga tcgagagctc
      181 ggcgatgaat ttcgggagtc cgttttccga gcaaagcgtt ccattggggc ccagggtcta
      241 tctcggcaag attgagatcg atgccatcca cgctttgcgg atgctgctcg atgtagcggc
      301 ggatcatttc cttgtacttg gcggtggctt tttcatatct tgccggattc tttactttat
      361 ctgaggcttt cactttggat ttgggaaaat ccgcggattg atcgcctgcg tttcgatcct
      421 tataggccga gaaaaattcg ggaatatcca tgacgtctcc cggatgcagc ttgcgaatga
      481 agtcttctcc actcagcgtc acgaattggt tcttcttcat ccaggctttc aattggggcg
      541 aataggaaga ggagtcgagt ttgtcaatga atgtcctcat atccggtttc ggatcatccg
      601 catccgcttc cttcccaatt tcctcgaagc tttttctcaa gagatagaaa gggaacccgc
      661 gtaccggctc ttccagtccg cccgagggag tggcatgtgc cacgaactca atcgagccat
      721 cttgcgcagt ggctcgcggc acgaccgcga tcgtcgccgc tatcaggatc gagataaagc
      781 tgactttgct tttcatggcg gccatcgtac cagagcggta caaccggggg ctgcgcggcg
      841 cggaacgatg ggtacagttg gttaacgtcc gacttggctg atgctcatgg aaaacaaaat
      901 cgggagggcg aaagtatact cgcgccttca gaacaactga aacgcaactt cgataatctg
      961 ccgcaccgca gcgggtctcc catcgggccc gcgcgcaggc gtgagccgcc aggtccgcac
     1021 cgcctggata gccttttcat cgaggccaag accaagtccc tttgccacgt gaatgtcagt
     1081 tacgcgacca tctgcggtaa cgatcgcaat cagctcgacc acaccttgca ccttcacctt
     1141 catggcttca tcgctataat cggctcgtgg acagtagagg catgccggaa cgccgtagcc
     1201 gccagtcccc gcattaggag ctccgccgcc ggtattccag ccctcgccgg gcccgacgcc
     1261 ggcaccgttt ccgtttccca cgcccgagcc atgaccttgc cccatcgaat cgctcaggga
     1321 cttgccaagc gggtcgcccc aattcggcag attctgattc ggcaatttga tatccgggtt
     1381 gccgaccacg gttggcgtca tcgcgatctg cgggtgctcc acgggatgcg ccatcggcgc
     1441 agagaattgc gtccacgaga acttcggcaa tttccccttg gcagccggtt ctgtgttgtg
     1501 cgcgcctcca ccaccacctg cggccttcgc ggaaggtttg agcttcgcga gatatggcga
     1561 aacgctcacg tctaccggcg gctccgggtt gtaagctttc gtcgtcggcg gcgagatgag
     1621 tcccggcagc agcagcggaa tcagcaccac cagggcaatc gctaccgcat ggacaagaat
     1681 cgaaaccgcc tgcacacggt tgaattgtgt attctttgac caaatttcgg gaacgggaat
     1741 gggttggctc gtggtctgaa gtgggggcag tttgcgaggc gcgatccagt cacgcagatt
     1801 ctgccaaagc gtcggctcct ctttccaatt caccagtagt gctgacttca cccttccacg
     1861 gggcgcggga tggaagaatt ccttcaggtt ggcgcccagg ccggtgccaa acttaggcat
     1921 gtcaaacgcc gtgggtgtgc cggggcgaac cttggcaggg cgctctgtca gaaagtctcg
     1981 tagattcgaa tataaacttt tgaggctgga ctcatgaacg ggcgcagcca agcgccccag
     2041 gcgcaggccg ggttcagttg atccgctcgt tgagcggtcg cctttgtccc gtctggtgct
     2101 aagatcgaac gttcgcattc cttgtatccc aaccgcagca gtacctcatc ttagacgcac
     2161 ggctgcgcaa aaggatgtct ggccccggac ggctgacaca cctcaggatt cattgtaaca
     2221 ttacgtggcg cgatgccaaa tttcggtttc caccgcgcaa attcgcagca caagctaaca
     2281 tatcccaacc atggaatgtg gcggggcgtt tcagaaaggg gattagcgaa tctcgaaaga
     2341 tgacgaaaac tgtcgatctg aaaagtaccc taaacctccc caaaaccgag ttccccatga
     2401 aagcacgttt gccggagcgc gagcccgacc agctgctcgc ctgggaagaa atggggcttt
     2461 acgcccgcat tcttgagcaa cgcgctggtg caccgctgtt cgtgcttcac gatggccccc
     2521 cctatccgac gggtgagatt catctgggta cgggactcaa caagatcgta aaggacatga
     2581 ttgtgaaatc gaaaaccatg gccggattcc gatcgcccta cgtccctggc tgggattgcc
     2641 acggtttgcc gattgaaacg caggtggaaa aggaattggg aggaaagacc acaaaagtca
     2701 gcgccgtcga atttcgtcgt ctgtgccgtg aattcgcggc acgatatgtg gaagcgcaca
     2761 agcgtgactt caagcgactc ggtgtctttg gccggtggga cagaccctat ctcaccatgg
     2821 accccggtat cgaagcttgc attgcaagcg ctttcatcga cttcttcgaa aaagggtacg
     2881 tctatcgagg gctgaaaccc gtctactggt gtattcgtga tcggaccgct cttgccgaag
     2941 cagaagtgga atacgaagat cacaccagcc cttccatttg ggtgaaattc ccgattgtac
     3001 ccgggccgaa aacacccgca ctcggcaacg acgtgaacgc gttgatttgg acgacgactc
     3061 cgtggaccct tcccgcgaat cgcgctctgg cttttcatcc ggatttcgaa tacacagtcg
     3121 cggagactac cgccggcaaa ctcttggtgg caaaagctcg catcgcagcg atcttcgatg
     3181 aactgaagct cgagatcggc aaggtgcacg gctcatggaa gggtagcgaa ttggaaggca
     3241 tccaattcca gcacccgttt ctcgacctgc gagtccctgc tgtgctggcg gatcacgtca
     3301 cgctcgatca gggcagcggc atcgtgcaca ccgcgccggg ccatggcgcg gaggacttcc
     3361 gcacgggcca aaaatacggc ctcgaggcgt atgccccaca ggacgatgac ggccgtttcg
     3421 tcgaaggact gccggaatac aaagggaaaa ctgtatggga ggccaacccg atcattattg
     3481 atctgttgat gaaccgcggc atgctcgttg gcggtcggca gttcacgcac agttatccgc
     3541 attgctggcg ctgtcacaat ccggtgattt tccgcgctac tgagcaatgg ttcgtggggc
     3601 tcgatcatga tggattgcgg cagaatgctc tcactgaaat cgcgaaagtg aagtggaagc
     3661 cctcctgggg tgaggctcgg attcgtgaga tggtggccga gcggccggat tggtgcattt
     3721 cccgccagcg cttttgggga gtccccctca tcgttttcac ctgtgaatcg tgcggcgaac
     3781 aactgaagga ttttgatgcg ctgaggcacg tgctgccctt cttcgagcgc gaaggtgccg
     3841 acgcatggta cgcccacacg accgaagagt tgctcccacc ggagacgaaa tgctcctgcg
     3901 gctccgcaaa gtggcgcaag gaaaacgaca ttcttgatgt ctggttcgac tcgggctcca
     3961 ctcacttagc cgttctcagc gaaaaggacg gcacctggcc cgcggacgtt tatatggaag
     4021 gccccgatca atatcgcggt tggttccaaa gctcgttgct catcggagtg ggcacgcgcg
     4081 gccgggcgcc gtaccggcaa gtcgtcaccc acggctggac gctcgacgaa aaaggcgcgc
     4141 cgatgtcgaa atcgctcggt aacgccatgt atccgaagga gatttgtgac aagtggggag
     4201 ccgacctgct gcgcgtatgg gtgtcttcac aggacttcac gcaggacgtt cgcctttccg
     4261 atgcgatgat gaagcaactc gcggaggcct accgaaaaat ccgcaatacc tttcgtttcg
     4321 cgctctctaa tcttttcgat ttcgaacccg ggcgcgacat cgtccacgat gcggaaatgt
     4381 gggaaatgga cgcctggatg cttcgccgca cgggagcgct tatccgtcaa tgcaacggat
     4441 ggtacaaaga atttgagttc caccgcgtct atcatgcgat tcaggatttc tgcacggtcg
     4501 atctgagcgc gttttatttc gatgttctca aggatcgcct ttataccttc gcacccaata
     4561 atcgtgggcg tcgctccgcg cagacggccg tctatcacat cgcacgggcg ctggtccgcc
     4621 tcgttgcgcc aatcctcgtt ttcacatccg aggaaatttg gaaatatctg ccgcggggcg
     4681 cttcggctca gccgagcgtg cacatggcct catttccctc tcccgaacca tttgagaagg
     4741 ccttcgacga tgcacgcgca aaagaatggg atcgcttgct cgtcgtgcgc gaagaagtga
     4801 tgagagcact cgaatctctg cgcgccgcca agactatttc cgccggcctg gaagcgcgag
     4861 ttacgctcac cgccaaaggc gatcttgcgg cgctactgca tagccatgtc gccagcctgc
     4921 cggcattctt catcgtttcg caagttgaga tcgccacaga taattcggat ggaggcaagc
     4981 cggctgccgg tgttgacggc gggttgcgta ctcacgtcga gcgcgcccat ggcaaaaagt
     5041 gcgagcgctg ctggaactat tccacgcacg tcggcgagaa cgcggattac ccaaattggt
     5101 gcgagcgctg cgtcgccgca atggatgaaa ttgagcgcga cggaggcagc gtggcgggga
     5161 gtgccgcctc gtaatgcgtt ccgtctcacg cccgggatgg gtcctgaccg tccttgcagt
     5221 agccgttctg attctggacc aggcaagcaa gtacgccgtt cagaatctaa ctccactggg
     5281 atcgagccgc gtcttgattc ccggcgtcct gaatctcgtt cattcaacca atccgggcgt
     5341 ggcgttcgga cttttggctg actcccaaat gccctggcgc gcgcctttgc tgatcgtgtt
     5401 ttccgtggcg gtgatcggat tgattacgtg gctgctggtc accgatcgcg ccggggaccg
     5461 gctgggccaa tggggtatgg cgttgatcct cggtggcgcg gcaggcaacg ttctggatcg
     5521 catcgtccgc cacagcgtta cggatttcat cgatttctac gtcggcagtc accactggta
     5581 cacttttaac gtcgctgact ctgcgatcgt catcggtgct gccttggtgc tgctcgaact
     5641 ccttcgcgat tggcgccatc ctagccagga acgggcctag ctatgcatcc tgtccttttt
     5701 caaattggct ccatcaccat ctatacgtac ggagtgctgg ccgcggctgg atttctcgtt
     5761 ggactctggc tcgcctaccg gcaagccccg cgcgccgggc tgaatcctca cagcgtatgg
     5821 aatcttggag tctatggcat cctcctggcc ttgctgctct cgaaggtgtg gctgatcttc
     5881 agcagttggg attactacgt cgcgcaccct gggcaaattt tcagcatggc tactttccag
     5941 tccgctggaa cattttacgg cggcattctt ggcggaattc tctgggttgt cttgtacacg
     6001 catttccaaa aaatgccgct tctcgcggtg ctcgatgtgg tcgccgctcc tgtcgcgctg
     6061 ggacatgcaa ttgggcgcat tggatgcttc gtggcgggtt gttgctatgg caagccgacg
     6121 tcactgccgt ggggcgtcac tttcaccagc cccgtcgcgg aaaaaattgc gggaacgcct
     6181 ctgcacattt cgctgcatcc cacgcagcta tacgaggccg gcgctgaatt cttgaacgtt
     6241 ctccttctcg tctggttagg cgctcggcag cgatttgcgg ggcagttgat cggcgcgtat
     6301 ttcgttctct acggaatcga gcgcggcgtc atcgaatttt tccgcggcga tcccggccgc
     6361 acaatgatgt tccacgatgc cgtgtcgcta atgcaaattg tgagtgccgt gctgatcatc
     6421 gcaggcggct cgctctggtg gcgcggcttg catcgcattg attcgacggt accggccccc
     6481 acagcgcgcg cgatggcggg ccgctcttag caatgcttgt ttgacgtgat gaaacaacgg
     6541 catgtccttc agcctctcag tccaacacct cgaatttacc gtgccgcctg actgttcggg
     6601 ccagcgcctt gaccgtttcc tcgtctccca gctttccgaa ctgagccgct cccgaatcca
     6661 atcactgatg gatcagggcc gcgtgctcgt ggacggcgtc gcacgcaagg cctcctatca
     6721 cgtcgcatcg ggcgagacac tcggcgtgga aatcccgcca actcctgcag cgggagtcga
     6781 ggccgaacct attccgctcc acatcctcta cgaagatgaa gacgtcgccg tcatcaacaa
     6841 gcctgccgga atgatcgtgc atcccggggc cggcgcggac gccggtacgc tggtagccgc
     6901 gcttctcaac cgcttcggcg gcagcgccgg tctctcgacc attggcggac cgctgcgtcc
     6961 cggcatcgtg catcgcctcg acaaagatac gtccggcgcg atcctgatcg ctcgcaccga
     7021 cgaagctcac cgtaagctgg tggaggattt tcgcgagcgt cgaatcgata agacctacgt
     7081 cgctctgctc cacggaaaaa tcaagggcga ctcaggctcc gtagaattgg ccatcgcccg
     7141 cgacctgcgc cgccgttccc gcatgacggc ccggcgtcgc gtgggccgcg aggcacggac
     7201 cgattggcgt gttctactcc ggctaaaagg ccacaccttc atcgaagcca gcttgtacac
     7261 cggcagaacc caccagattc gcgttcactt ttctgcgctc gggcatccgg ttgtaggcga
     7321 tacgctctac ggtgccccgc gccaggaacg cgctgcctct gcgctgctcc caccgctaaa
     7381 ccgcaatttc ctccatgctg cccgcgtcgg ctttgtccat ccacgaacag gtcggcgaat
     7441 cgaagttcgc gcgccgctcc cgccccagct cgtggactat ctagcctttc tcgcacgtag
     7501 cgtccaagtt gatccccatt cggttgacgc tgccctccgc gaattcctat aattctcccg
     7561 tgaagtgccg cgtgatgaat cgtcctggct ccctccggac agcagtgttt gtgctgctca
     7621 tcctattcgc ggtctgcggg atttgcgctc gtgcgcaaga ggctccatcc aaacccgtgg
     7681 ccccggccac ttccgcccct cagcaggccc cagctacgcc caatccgcaa gcggccgcac
     7741 ctcctcagca gccaccaatc gtcagcacca cgggcctcgt ccacctcgtc gttacggttg
     7801 tggatcgcca tcacgacttc atcaccgatc tcgataagac cgatttcaag gttctcgata
     7861 acggttcccc ccaag
//
LOCUS       DATNDT010000370         7084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1521298, whole genome shotgun sequence.
ACCESSION   DATNDT010000370 DATNDT010000000
VERSION     DATNDT010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1521298"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="VN788_11885"
     CDS             complement(<1..1364)
                     /locus_tag="VN788_11885"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXU21273.1"
                     /translation="MNRIRTITLKWASTLMLFAVIQPAMGQQSAPALSSLHTDTASAV
                     LLQAMSDELERSKSQLKIENVPAPYYIEYRLSDSDEYDAEAAFGALRVDQRIHARSVR
                     VVVRVGDYKQDSYYGPGVGTVSLAPIDNDAMALRWQLWIATDQAYKAASEALAMKNAA
                     LRQYTAGQPFNDFARAPALQSIEPVAKLDFDPNPWKQMIEKSTALFRRDPKIESLSAG
                     IRFRAVNQYFVNSEGTVTRSGYTVYSLHLFASTQADDGMRLGRSPDYIAGSLKELPTP
                     DQFQADTLKMLDTLKNLREAPVVDEDYRGPILFSADAASELFEGMVGRNLLGIRPRPG
                     DSARTTGDYASKFKSRVLPAFLSVIDDPTQQSFGGKTLVGAYAADDEGVRASKVPVIQ
                     DGMLVNYLLGREPIRDFPDSNGHGRAGPGQLASPNLGVLMIESKQASSPAELKQKLIE
                     ICRQ"
     gene            complement(1361..3100)
                     /locus_tag="VN788_11890"
     CDS             complement(1361..3100)
                     /locus_tag="VN788_11890"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXU21274.1"
                     /translation="MSRSLFRPAVCFLLIWSGICFAGISSTSGPDILLQTMQRELERA
                     TSSLKRADPSPYFLSYTVADTAGATIVGTNGSLVLSTNNRRRLADVMMRVGASALDNT
                     HGQSRPSAITSGTLPLAGDSDATARVLWQLTDHEYEQASGAFLKVKTNQAVQSEEEDK
                     SPDFSQETPRTHIDESRPAVSFDQKLWEDRVRRVSARFLKYPAVYTSLVMLQQGLDRF
                     YLATSEGTALVQPSAIARLVIEGETRADDGMDLLRVESFQETSPGELPSESELIAKVD
                     KIAADLNALRAAPVAEPYAGPALLSGRAAAVFFHEVLGHRLEGHRQRGESEGQTFTRK
                     VNQQVLPQFLTVVDDPTMNQLNGVKLAGHYEFDDEGVPAQRVDAVDHGILKNFLMSRM
                     PITNFSHSNGHGRRQAGLMPTGRQGNLIVTSSKTVKDSDLRQRFIEEIRKQGKPYGLY
                     FEDIQGGFTLTGRATPQAFQVIPVMVWRVYPDGRPDQLVRGVDIVGTPLLSLNNIILT
                     GDTLQVFNGICGAESGQIPVAAAAPAMLFSEIEVQKRQKGTQRPPLLPPPSFASLPAP
                     SHAQSVPQVEPRR"
     gene            complement(3239..4312)
                     /locus_tag="VN788_11895"
     CDS             complement(3239..4312)
                     /locus_tag="VN788_11895"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HXU21275.1"
                     /translation="MTIPSDLIAFAVALVGSLCLTVPVRRFALQYGMVDHPGPRKVHL
                     RPVPLLGGIAIYLGFVLAILLTLHAVPQQQMTSILAGATLLAVVGGMDDGRLLHHQVK
                     LFVGMPAAALFLLASGIRADIFARFIPGFGGLILDGCLTMFWVVGITASFSILDHMDG
                     LCAGISAVGAIFFFLSASFSGQTMVRTLAAATLGAALGFLLWNFNPAKIFMGDGGAMF
                     LGFLMAALGLKVRPEGIHGSVSWLVPVLILGVPIFDTTLVSISRARRSLLPFTSPGKD
                     HTAHRLSNLGLGHRGAVLALYAVAVLFGIIALAIPHVSVGTAYMIAAVAALCGIVAVI
                     FLERAPYERQVKPRKSVPSLQLQ"
     gene            complement(4372..5562)
                     /locus_tag="VN788_11900"
     CDS             complement(4372..5562)
                     /locus_tag="VN788_11900"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU21276.1"
                     /translation="MAAQVAETLAQHHNVTVVAGRPSYDPDEFYPWSLLRSDLRNGVH
                     VERVGSTAYPRHQMRRRVSNYLSYLALAVPRVLTTQADVVLAMTDPPIAGIAGAFVAR
                     ITRRPFVYSISDLYPEMAVAGNIMKPSIWTHRWEKMHRRALKQAARVIVLGDDMRERI
                     VAKGVSPQRVTVVRSGASLPSSTPDPGDPIVSKIVHEIRCGFPFVVLHAGNLGFYGAW
                     NALLGAAEILRDENVGFVFVGDGANRAALEASASNSSNVRFLPFRPAEQIPHVMMAGD
                     IQIVTIRRGLEGVVVPSKLYSTLAMGRPVLAVASSSSDAARIVLESGCGLAADPDDPV
                     AIAAAIRELRSDAGRLALMGHRSREAAGKYARLKELRHFSAIVDQVGFEAGIPHGKDV
                     QPCA"
     gene            complement(5626..>7084)
                     /locus_tag="VN788_11905"
     CDS             complement(5626..>7084)
                     /locus_tag="VN788_11905"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXU21277.1"
                     /translation="EIDPSAIRAQFHRVEHHRAHLASAFFVSPFDRAAVLSADGLGDF
                     ASFMCGVGEGPKITTLCEVAFPHSLGIYYTALTQYLGFWKFGDEYKVMGLAAYGQPAF
                     LDEFRRIVRTDPPLSFRLGLEYFTHQNNGAEMTWRDAARTPVIGRLFSAYLETRLGPA
                     RRPDEPLDQRHYDLAASMQAALEEVLVSCWNGLAKRSGQTALCLAGGVAFNCVANGKI
                     FDSTPFEHVYVQPAAGDAGLSVGAAFEVHHKLGGARGFTMDHAYWGPVFSSSDIRAVL
                     DHTFSSGEADISELAEAGLVQSTARHIADGKIVGWFQGAAEWGPRALGNRSILADPRR
                     PEMKDTLNRRIKHREAFRPFAPSIIEEAVGEFFEKSHPSPFMTFAYPVRPEKRSAIPA
                     PTHVDGTARLQTVSQSASPLYWKLLRAVGDLSGVPAVLNTSFNDNEPIVCRPEEALDC
                     FRRTRMDVLVMGNFLLERRSAAPEKAHGRLASHAD"
BASE COUNT         1667 a         1917 c         2108 g         1392 t
ORIGIN      
        1 tcctgcctgc aaatttcaat cagcttttgc ttgagttcgg ccggagagga agcctgcttg
       61 gattcgatca tcaagacgcc gaggttgggc gatgcaagct gtcccgggcc ggcgcggccg
      121 tggccattcg aatcggggaa atcgcggatc ggttcgcggc ccaggaggta gttcacgagc
      181 atgccgtcct gaattacggg aacttttgat gcgcgtacgc cttcgtcatc cgctgcatag
      241 gcgccaacga gagttttccc gccgaaggat tgctgcgtgg ggtcgtcaat gacggacagg
      301 aaagccggca agacgcggct tttgaactta gaagcataat ctcctgtagt gcgggcggag
      361 tcgcctggtc ttggccttat gccgagcagg ttgcggccga ccattccttc gaaaagctcg
      421 gaggccgcat cagcggaaaa aagaatgggc ccgcgataat cttcgtcgac tacgggggcc
      481 tcgcggagat tcttcagagt gtcgagcatt ttcagggtat cagcttggaa ttgatcggga
      541 gtgggcaact ccttcaacga tcctgcaatg taatcgggcg atcggccgag acgcatgcca
      601 tcgtcagcct gagtggatgc gaataggtgc agggagtaaa cggtgtagcc ggacctggtt
      661 accgtgcctt cgctattcac gaagtattgg tttacggcgc ggaagcgaat accggctgaa
      721 agggattcga ttttcgggtc gcggcggaaa agggcagtgg acttctcaat catttgtttc
      781 cacggattgg gatcaaagtc gagcttcgcg acgggctcga tggattgaag cgcaggagcg
      841 cgagcgaaat cgttgaacgg ctggccagcg gtgtattgac gcagagcagc gttcttcatg
      901 gccagtgctt cgctggcagc tttgtaggct tggtcggtgg caatccacaa ttgccagcgg
      961 agagccatgg catcgttatc gatcggagcc aggctcaccg tgccaacacc gggtccgtaa
     1021 tagctatcct gtttgtagtc gcctacgcgc acgaccacac ggacggagcg tgcgtgaatg
     1081 cgctggtcca cgcgaagcgc gccgaaagcg gcctcggcat cgtactcatc cgaatcggat
     1141 aggcggtact caatatagta gggagcaggg acattttcga ttttgagctg cgactttgaa
     1201 cgctcgagtt cgtcactcat cgcctgcagc agaaccgcag aggcagtatc ggtgtgtagg
     1261 gaacttagag cgggagcgct ctgctggccc atggccggct ggataacagc aaatagcatc
     1321 agagtggaag cccacttcag cgtgatggtc ctgattctgt tcatcgcctg ggttccacct
     1381 ggggcacgga ctgcgcatgc gacggcgctg ggagcgaggc aaaactgggt gggggcagca
     1441 ggggcgggcg ctgcgttccc ttctgacgtt tttgcacctc gatttccgag aagagcattg
     1501 cgggagcggc ggcggccacg ggtatttgac cggattccgc tccgcaaatt ccattgaata
     1561 cctgcagagt gtctccagtg agaataatgt tattaagaga caacagcggc gtgcctacga
     1621 tatccacacc acgcacgagt tgatcgggcc ggccatccgg atagacgcgc cagaccatca
     1681 caggaatcac ctggaatgcc tggggagtgg cacgccccgt tagcgtgaag ccgccctgta
     1741 tatcttcgaa atacagccca taaggtttcc cctgcttcct gatctcttcg ataaatcttt
     1801 ggcgcagatc ggaatccttt accgtttttg aggaggtgac aatcagattg ccctgccggc
     1861 cggtgggcat caggccggct tggcgacggc cgtgtccatt tgaatgactg aagtttgtaa
     1921 tgggcatgcg agacataagg aaatttttca gaattccatg gtccaccgca tcgacccgct
     1981 gggccggaac accctcatca tcaaactcgt aatggccagc cagcttcact ccgtttaact
     2041 ggttcattgt gggatcatcg acgaccgtca aaaattgagg cagaacctgc tgattcacct
     2101 ttctcgtgaa ggtctgccct tcgctttctc cgcgctgtcg gtggccttcg agcctatgtc
     2161 ccagcacttc atgaaagaat acggcggcgg ccctgccgga gagtaaggcc ggtccagcat
     2221 atggctcggc gacaggcgcc gcgcgcaatg cgttaaggtc tgccgcgatt ttgtcgactt
     2281 tggcgatcag ttcactttcg gagggaagct cgccggggga agtttcctgg aaagactcaa
     2341 cgcgcagcaa gtccatgcca tcgtcggccc gagtttcccc ctcgatcacg agacgcgcaa
     2401 tcgccgaagg ctgcacgagt gcggttcctt cgctagtggc aagatagaag cggtcgaggc
     2461 cctgctgtaa catgactaaa gacgtgtaca cggcggggta ctttaggaat ctagctgaca
     2521 cgcgccggac gcggtcttcc caaagtttct gatcgaagga aaccgcaggg cgggattcgt
     2581 caatgtgagt gcgaggcgtc tcctgagaaa aatccggaga tttgtcctct tcttcggatt
     2641 gaacggcctg gttcgtcttc actttcaaaa aggcccccga agcctgctcg tattcgtgat
     2701 ccgtcaattg ccagagaacg cgagcggttg cgtcggaatc acccgccaag ggaagagtcc
     2761 cggaagtaat tgcggaagga cggctctggc cgtgcgtgtt atcaagcgca gaagcgccca
     2821 cacgcatcat cacgtcagcc aaccgccgcc ggttgttcgt ggagagaacg agactgccat
     2881 tcgtgccgac gattgtggcg ccagccgtgt ccgcaacggt atagctgaga aaataaggcg
     2941 aggggtcggc tcgttttagg gagctggtag cgcgctctaa ctcccgctgc atcgtttgga
     3001 gcagaatatc gggaccggat gtggaagaaa tccccgcgaa acagataccg ctccagatca
     3061 acaagaaaca aacggcgggc cgaaaaaggg agcggctcat aagtccatta attgctgaat
     3121 gaaggccaat ctaacacact tgtcagagga acaaggaaga aacagaggtc tagtgctctg
     3181 aatagaacca acaattcgtt agtcggcgcg aaagctgcag agaaataaga cggagagatt
     3241 attggagctg aagcgaaggt acggatttcc gaggctttac ctggcgttca taaggggccc
     3301 gctcgaggaa tatcacggcg acgattccgc aaagtgccgc gacagccgcg atcatgtagg
     3361 cagttccaac agagacgtga ggaatcgcca gagcgatgat tccaaacagc actgccacgg
     3421 cgtaaagagc aaggacggcg ccgcggtggc caagccccaa gttcgacaac cgatgcgcgg
     3481 tgtgatcctt gcccggcgaa gtgaaaggca gcaagctgcg acgagcgcgt gatatcgaga
     3541 caagggtggt atcaaagatc ggtacgccca aaataagaac aggaaccagc cagctgacgg
     3601 acccgtgaat tccttctggc cgaaccttca ggccgagagc ggccatcaag aagcccaaga
     3661 acatggctcc tccgtcgccc atgaaaattt ttgctggatt aaaattccaa agcaaaaagc
     3721 cgagcgcggc tccgagggtg gcggcggcca gcgtgcggac catggtttga ccgctgaaac
     3781 ttgcggataa aaagaaaaag attgcaccga cagccgatat accggcgcaa agaccgtcca
     3841 tgtgatcgag gatgctgaag gacgccgtaa tgccgacaac ccaaaacata gtgagacagc
     3901 cgtcgaggat taagccgcca aagccgggaa tgaaacgagc aaagatgtcg gcgcggatgc
     3961 cggatgccag cagaaacaaa gctgccgccg gcatgcccac gaaaagtttg acttggtgat
     4021 gcagcaagcg accgtcatcc attccgccaa ctacagccag cagtgtggcg ccggccagaa
     4081 tgctcgtcat ttgctgctgg ggcacggcat gtaacgtcaa aagaattgcg agtacgaatc
     4141 caagataaat ggcgatccca ccgagaagag gaacggggcg caagtggact ttgcgcgggc
     4201 caggatgatc taccatccca tactggagag cgaagcgccg aacgggaacc gtcagacaga
     4261 gcgagcctac cagtgctact gcaaaggcga ttaggtcgga aggaatggtc atcgaattcc
     4321 aatgttaatc atgtgtgccg gtaaactgct cgtcacctga agacgcctgg atcacgcaca
     4381 cggctgaaca tctttcccgt gaggtatgcc agcctcaaat ccgacttggt ccacgattgc
     4441 ggaaaaatgc cgcaactcct ttaatctagc atacttccct gccgcttccc gagagcgatg
     4501 tcccattagg gctagacgtc cagcatcgga gcgtagctcc cgaatagcgg cggcgatggc
     4561 gacggggtca tcgggatcag cagcgagccc gcaaccagat tccaagacaa tccgggctgc
     4621 gtcgctggat gaagatgcga cagcaaggac cgggcgcccc atcgcaagag tcgaatagag
     4681 tttgctcggc accactaccc cctcgagtcc gcggcggatg gttacgatct gtatgtcgcc
     4741 agccatcatt acgtggggga tctgctcggc cggacgaaat ggcagaaagc ggacgttaga
     4801 agaatttgag gcagaagctt cgagcgccgc gcggtttgcg ccgtccccga caaacacaaa
     4861 tccgacattt tcatcacgca agatttccgc ggccccaagg agggcattcc aggcgccgta
     4921 gaagcctaga ttgccggcat gaaggacgac gaacgggaag ccgcagcgga tttcatgcac
     4981 gattttcgag acgattggat cgcctgggtc tggcgtggag gagggcaagg atgctccact
     5041 gcgaacgaca gttactcgct gggggctgac acccttggct acaatgcgct cgcgcatgtc
     5101 gtcacccaaa acaattacgc gagcggcttg cttgagggcg cgacgatgca tcttttccca
     5161 gcgatgggtc caaatgctgg gcttcatgat gtttcctgcg acggccatct ctggatagag
     5221 atcggaaatg ctgtaaacaa acgggcgacg cgtgatcctc gcgacgaatg ccccggcaat
     5281 cccagcgatc ggtgggtccg tcatcgccaa aacaacgtcc gcctgagtcg tcaggacgcg
     5341 gggtaccgcc aaagcgagat atgacaagta attcgagacg cgccggcgca tttggtggcg
     5401 tgggtaggct gtggatccga cacgctcgac atgcacgccg ttgcgcaggt ccgaccggag
     5461 caatgaccac ggatagaact cgtcgggatc gtaggaaggg cggcccgcca cgactgtcac
     5521 gttatgatgc tgcgccaggg tctcggcgac ctgtgccgcc atctttgcag ttgccgacgt
     5581 atccgggggg tagtattcgt tcaaaagcaa aatgtgcatg cgagtttagt ctgcatgact
     5641 agccaagcgt ccatgggctt tctcgggggc ggcggatctt ctctcgagaa ggaaatttcc
     5701 catcacaaga acgtccatcc gggttcggcg aaaacagtca agcgcctctt cggggcggca
     5761 gacgatcggc tcattatcat tgaacgatgt gttcaaaacg gcaggcacgc cgctcagatc
     5821 gcccacggcg cgaagaagct tccaatagag gggactggcc gattggctaa ccgtttgtaa
     5881 acgcgctgtg ccatccacgt gagttggcgc gggaatcgcg gagcgctttt ccggacgcac
     5941 cgggtaggcg aaggtcataa aaggcgatgg atgagacttc tcaaaaaact ctccaactgc
     6001 ctcttcaata attgaaggtg caaacggacg aaatgcttct cgatgtttga tacgacgatt
     6061 cagcgtgtcc ttcatttcgg gacgtctggg atcggcgaga atgctgcgat tacccagagc
     6121 tctgggtccc cattcggctg ctccctgaaa ccagcctaca atttttccat cagcgatgtg
     6181 tcgtgcggtt gattgaacga gcccggcttc ggcgagttcc gagatatccg cctcgccgga
     6241 cgagaaagtg tgatccaaaa ccgcccgaat atcagaagaa gaaaataccg gaccccaata
     6301 cgcgtggtcc atcgtgaatc cgcgagcgcc tccaagcttg tgatgtactt cgaacgccgc
     6361 gccgacggag agtcccgcat ccccggccgc aggctggaca tacacatgct cgaacggagt
     6421 agagtcaaaa atcttgccgt tggcgacgca gttgaaggct acccccccag ccaaacagag
     6481 cgccgtctgt ccactgcgct tcgcgaggcc attccagcaa gacaccagaa cttcttctaa
     6541 agcagcctgc atgctcgccg ccagatcgta atgccgctga tcgagcggtt catcaggtcg
     6601 tcgcgctggt ccgagccgtg tttcgagata ggcggaaaaa agccggccga ttaccggcgt
     6661 gcgggcagca tcgcgccaag tcatctccgc gccgttattc tgatgcgtaa agtattcaag
     6721 tcccaagcga aaggaaagcg gcggatccgt acgaacgata cgccgaaatt cgtcgagaaa
     6781 tgcaggctgg ccataggcgg ccaggcccat caccttgtat tcgtcgccaa atttccagaa
     6841 gccgagatat tgggtgagtg ctgtgtaata tatgccgagc gaatgaggga acgccacctc
     6901 gcaaagcgtg gttatttttg gtccttcgcc cacgccgcac atgaagcttg cgaagtcgcc
     6961 caaaccgtcc gcagagagca cagctgcccg atcgaatggc gacacaaaga atgcgctcgc
     7021 aagatgggcg cggtggtgct cgacgcggtg aaattgagcc cggatcgcac tcgggtcaat
     7081 ttca
//
LOCUS       DATNDT010000371         8966 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2662073, whole genome shotgun sequence.
ACCESSION   DATNDT010000371 DATNDT010000000
VERSION     DATNDT010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8966)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8966)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8966
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2662073"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..1243
                     /locus_tag="VN788_11910"
     CDS             38..1243
                     /locus_tag="VN788_11910"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU21278.1"
                     /translation="MIRRLIPAWIVLAIALLLPTIAAAQDVRVTGQVMDRDGKPWAGL
                     TVVIKSDSGRSFTLKTDNQGKFSQIGLTPGLYSVSVDLPGQQQPYTEKHQFSSGGDNN
                     VTINFKAILAQQGANPEQEKKQQEAANQFKNMKAHVDAGVAALTSAEDVRKQLKTASA
                     DQKSALEGQMKADYQTAVTELQQAEQASAANPKDTKNHAIILSDLGVAYSNSGQYDQA
                     VSSYQKAIELNPQAGYYNGLSSSLANSAASQTDPAAVTSKISDAGAACDKATALQTPP
                     NPTDTARCWKNIGIVLSNKGDLKDAIAPLQKATQADPKDAQGWYLLGSAFTGTIDSKQ
                     EGDKMTYIIPPGTNDAYQKCIDADPNGPYAAQCKTMLDSLAQMSGGASTMVGERRKKE
                     TKKENKKSH"
     gene            complement(1755..2315)
                     /locus_tag="VN788_11915"
     CDS             complement(1755..2315)
                     /locus_tag="VN788_11915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21279.1"
                     /translation="MYLLDIEALDLGPEVRAVSLDLVEADENREPVRGEDAAKIWSRV
                     IRATAGSERWALDFFSHLDRVRDFCDGHGIEYREPNKRSVVIPAPEGDSLEGLIGRFQ
                     GETFGVRAGGALAGDGKDPALEGDLARRGADAYHHAFPNYYFCAICGFEDGSLVVLSE
                     KLWASEIIRRARPAVRDLKVEIRLPV"
     gene            complement(2316..2903)
                     /locus_tag="VN788_11920"
     CDS             complement(2316..2903)
                     /locus_tag="VN788_11920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21280.1"
                     /translation="MSASPPSKKSSSNPPTKKVLILEAARAMSKPRFTPAEIEQIRRQ
                     LIARLGARGNTGADYIVSVLKEGGLRVAWSTRSDTEGRYEKEFDDLLHFSTLDEAEMC
                     LVRLDELLRKFAAEGERAASERVREVALLGRRRAEMIARSSKVDAQKRAEKEEVAHWF
                     AIWLETPGAFFDWLELRKQSPEFQRQFPADADDEA"
     gene            complement(2900..3964)
                     /gene="mltG"
                     /locus_tag="VN788_11925"
     CDS             complement(2900..3964)
                     /gene="mltG"
                     /locus_tag="VN788_11925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HXU21281.1"
                     /translation="MRRLGLLALLAISALGAGAAWLDSQISRPYRGHRPQQVFVDVPK
                     GTSRWGVAGILRRDDVIRSRLAFALFSDWHFRRALQAGEYYFDQPVNSREVFWKISHG
                     RIFVRTVLIPEGWTIYNIAAEIEQQKICSAQEFIAAAHDTALISDLDPQAQSLEGFLF
                     PSTYEFTRHSTCQQVVKRMVQNFRAVWETMDTGSSRPLSLGFTPEQIVTLASLVERET
                     PSGEERPLVAGVFYNRLRLKIPLQCDPTVQYAMRLAGHAEKDVRPQDLRIDSPYNTYE
                     HPGLPPGPIANPGEASLRAALSPANTDYLYFVANDDGGHFFSRTLAEHNRNVARLRRL
                     LGSNAKSDLASRKTPTGRKP"
     gene            complement(3971..4396)
                     /gene="ruvX"
                     /locus_tag="VN788_11930"
     CDS             complement(3971..4396)
                     /gene="ruvX"
                     /locus_tag="VN788_11930"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HXU21282.1"
                     /translation="MDYGRKRIGLALSDELGLTARPLTTMQRTNRQNDMRRLREICRR
                     NGVCRIIVGHPLHLTGEAGAMAKEALGFARRLGKAVNIKVEMRDERLTSWEARQMATE
                     IRSGRPREDPLDDVAAAILLRDYLGQKRDHGRSGSVEKD"
     gene            complement(4581..5741)
                     /locus_tag="VN788_11935"
     CDS             complement(4581..5741)
                     /locus_tag="VN788_11935"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXU21283.1"
                     /translation="MTKTMTAKIEGREYVGFRAMSIRPKRVEQKRWVPVVLAGVMLLF
                     ASICPAQVSPINPPSGWKVPNGAGTCAVGKSCADLAPAMMKSALGASPLEKNLRDLTS
                     RAGRGTKSSPGAKRAAGWAVEAFRQAGVDEVRTEKFTTPDGRSDGQAEGVAGAAGSEN
                     VVAEIRGREDPDEFVVLGAHLSDGPDTALVIDAARVIHSSGSVPRRSIRFVLFADGKK
                     GTPGAWAYTRAHHAELERTSAAILFDGGIGAISGYSLAGRKDALAPVREALAPLAPMG
                     VKDFTLGARIDADRLDFILEGILTLDPNQKPVNEHATSDVFDKANIAALKRQAGIAAI
                     TAYALADTSEPIVGRQSRAEIEQLLKETGLGQEMKNRGFWPAWESGERGRKQ"
     gene            6147..6776
                     /locus_tag="VN788_11940"
     CDS             6147..6776
                     /locus_tag="VN788_11940"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HXU21284.1"
                     /translation="MRDVSKLLTNKRKLERNQSNHFVFCEKRSGLAQFHGACELDRDS
                     TIERMASTLAMQCLVRGSEPDDFAILVPVDSILAGRLESRAKELLDEGRSIANPASLS
                     PRQQEILHSILCNHANKEIANKLNITVRTVKFHISTLLTKFGAENRSELARRASGFLR
                     TSMHVEDGFPNPSPGVRRDDLRPIPVDSQDRARPKAGNLRISQQRILTA"
     gene            complement(6869..7894)
                     /locus_tag="VN788_11945"
     CDS             complement(6869..7894)
                     /locus_tag="VN788_11945"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXU21285.1"
                     /translation="MKKIGFLSFGHWTAAPQSATRSASDALLQSIDLAVEAERLGMDG
                     AYFRVHHFARQLASPFPLLAAVGAKTRRIEIGTAVIDMRYENPYYMAEDAGAADLIAG
                     GRLQLGISRGSPEQVIDGWRHFGYQPAEGENDEDMGRRHAQEFLELLRGKGFAKPNPR
                     PMFPNPPGLLRIEPYSEGLRDRIWWGAATNATAAWAAKLGMNLQSSTLKFDETGEPFH
                     VQQAAQIRAFRAAWKEAGHTRTPRVSVSRSIFALVDDRDRAYFGRGGEEVDQIGFLSG
                     EPTRAIFGRSYAAEPDVLIEQLRKDEAIAEADTLLLTVPSQLGVDYNSHAMEAILTTV
                     APGLGWR"
     gene            complement(8286..8888)
                     /locus_tag="VN788_11950"
     CDS             complement(8286..8888)
                     /locus_tag="VN788_11950"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HXU21286.1"
                     /translation="MNRLMTATFAALLLCLSFYAQGQASGQATNQAPSPAPAAARPAP
                     APLAIPNPHYVSIKMEMDVNRPAAEVWKRVGKFCDIGEWFQVPCTILSGTDGEVGVVR
                     SIGNEVMVGKTELSYTYTQPVRVGQPYNLYHGTLEARPVTATTSKLVYTLVFDNSMLA
                     DDAAREGDIARRRTLFMNGLKNMKILAEGGTLPPRPARTP"
BASE COUNT         1836 a         2852 c         2377 g         1901 t
ORIGIN      
        1 gggggatgct gcatttcgtg attctgggga gaggaaaatg atcagacgac tgattcctgc
       61 ttggattgtt ttggccatcg cactcctttt gccgacaatt gccgccgcgc aggatgtgcg
      121 cgtaaccggt caggtgatgg accgtgacgg caagccgtgg gccggcctca ccgtggttat
      181 caaaagcgat tcgggacgtt cttttacgct gaagactgac aaccaaggca agttcagcca
      241 gattgggctg acgcccggct tgtatagcgt cagcgtggat ctgccgggcc aacaacaacc
      301 ctacacggaa aaacatcaat tctcttcggg cggagacaac aacgtaacga tcaatttcaa
      361 agccattctt gcgcagcaag gggcaaaccc ggagcaggaa aaaaagcagc aggaagcggc
      421 caatcagttc aaaaacatga aggctcacgt tgacgccggc gtagcggcac tcactagcgc
      481 cgaagatgtg cgcaaacagc taaaaaccgc ctcggccgat cagaaatccg ccctcgaagg
      541 ccagatgaag gctgactacc agacggctgt caccgaactt cagcaagccg aacaggcctc
      601 tgccgccaac cccaaagaca ccaaaaacca cgccatcatt ttgtccgatc tcggcgttgc
      661 ctacagcaat tcggggcagt atgaccaggc cgtcagctct tatcaaaaag ccatcgaatt
      721 gaatccccag gccggttatt acaatggttt aagttcttcc ctggcaaatt cagcggcatc
      781 acagacggat cctgctgccg tcacatcgaa aatctccgac gctggcgccg cttgcgacaa
      841 agccaccgcc ctccaaacgc cgccgaatcc aaccgatacg gctcggtgct ggaagaacat
      901 cgggattgtc ctcagcaaca agggagattt gaaggacgcg attgctccct tacagaaagc
      961 cactcaagct gatccaaaag acgctcaagg atggtatttg ctcggaagcg ctttcacggg
     1021 cacgattgac tcaaagcagg aaggcgacaa aatgacttac atcatccccc cgggcactaa
     1081 cgatgcctat caaaagtgca tcgatgccga tcccaatggg ccgtacgcgg cgcagtgtaa
     1141 aacaatgctg gattcgctgg cccagatgag tgggggtgca agcacgatgg tgggcgagcg
     1201 caggaagaaa gaaaccaaaa aagaaaacaa gaaatcgcac taaaactacg ggggcgttct
     1261 ccctgcgcag tggcggctct gatttgacaa caacgttggt ttctcggttt tccgtcgtga
     1321 tgttctactg ctcccacggg gagtggattc gagaatgatc tgcccgcagt gtcgcagcat
     1381 tgattgctat cgatcacgtc gtgctggcgc aatcgatact ttctgcaggc tgatcgggct
     1441 caggtcctgg cgatgctata cgtgcagctt tcggttttac gcgcgcagcg tggcggccgt
     1501 tttctccccc tacgctcatt gctcgcgctg cggcaacttc gatttgcagc gaatcgcccc
     1561 cgaccgcgtg ccgcctggca tcctaacgtc cgtcgagcga ttcctcggct tcgccgcgta
     1621 ccgttgcagc ccatgccggg ccaaattctt caccatcctt ccccagcgcc gcatccttcc
     1681 ctcgatgatg cctcccccgc gcttctgaaa agcagcgagt cctaaccggt cctgaaggcg
     1741 cccccgtcgt ccgctcacac gggcaggcga atttccactt tcaaatccct caccgcaggc
     1801 cttgcgcgcc ggattatttc actggcccaa agcttctcgc tcagtaccac cagcgaacca
     1861 tcttcaaatc cgcaaatggc gcagaaatag tagttcggga atgcgtgatg gtaagcgtcc
     1921 gcgccgcgcc gcgcaagatc accctcgagc gccggatcct tgccgtcccc tgccagcgca
     1981 cctccggcgc gaaccccgaa cgtctcgccc tggaaacgtc cgatcaaccc ttccagagag
     2041 tccccttccg gcgccggaat cacaaccgac cgcttgttcg gctcgcggta ttcaattccg
     2101 tgaccgtcgc aaaaatcgcg cacccgatcc aagtggctga aaaaatctag cgcccagcgt
     2161 tcgcttccag cggtcgcacg aatcacgcgg ctccagattt tggccgcatc ttctccgcgc
     2221 actggctcgc ggttttcgtc cgcttccacc aagtccaggc tcacggcgcg cacctcggga
     2281 ccaaggtcca gcgcctcgat atccagcaag tacatttacg cctcgtcgtc tgcgtcggcg
     2341 ggaaactgcc tctggaattc gggggattgc ttccgtagtt cgagccaatc gaaaaaggcg
     2401 ccgggagttt caagccaaat agcaaaccag tgagcaacct cttctttttc cgcccgcttc
     2461 tgcgcatcca ctttgctgct tcttgcgatc atctccgccc gccgcctccc cagaagcgca
     2521 acctcacgga cgcgttcgct cgctgcacgt tcaccttcgg ccgcgaattt ccgcagcagc
     2581 tcgtcgagcc gcaccaggca catttccgcc tcgtcgagcg tcgaaaagtg caacaggtcg
     2641 tcaaattctt tctcgtaacg gccttcggta tccgatcggg tggaccatgc tacccgcaag
     2701 cccccctcct tcagaacgct cacaatgtaa tccgcccccg tatttccccg agcaccgaga
     2761 cgcgctatca attggcgccg gatttgctcg atttcggcgg gcgtgaagcg cggcttgctc
     2821 atcgctcgtg cggcttcaag gatcagcact ttcttcgtcg gcggattgct cgaagacttc
     2881 ttactgggag ggctggcgct caaggtttcc tccctgtcgg agttttcctc gatgcaagat
     2941 cggatttcgc gttgcttccc agcagccgtc gcaggcgcgc cacgttgcga ttgtgttcgg
     3001 cgagtgtgcg gctgaaaaag tgcccgccat cgtcgttcgc cacaaaatat aggtaatccg
     3061 tattcgcggg cgatagagcg gcgcgcagcg atgcttcgcc cggattggcg atgggacccg
     3121 gcggcaatcc cgggtgctcg taggtgttgt agggcgaatc gatcctcaga tcctgcggcc
     3181 ggacatcttt ctccgcatgc cccgccagcc tcatcgcgta ctgcaccgtg gggtcgcatt
     3241 gcagcggaat ttttagccgc aatcggttgt agaacactcc tgccactagc ggccgttctt
     3301 ccccgctggg cgtttcgcgc tccaccaggg aagccaatgt cacgatctgc tctggcgtaa
     3361 atccaagaga caagggccgg gagcttcccg tgtccatcgt ttcccacact gcgcgaaaat
     3421 tctgaaccat ccgcttcacc acctgctggc acgtgctgtg gcgcgtgaat tcataagttg
     3481 aaggaaatag aaatccctcc aggctctgcg cttgcggatc gagatcggat atcagggcgg
     3541 tatcatgcgc cgccgcgatg aattcttggg cgctgcagat cttctgttgt tcgatttcgg
     3601 cggcgatgtt gtatatcgtc catccctcag gtatcaggac ggtgcggacg aagattcgtc
     3661 cgtgcgagat cttccagaaa acctcgcgcg agttcaccgg ttgatcgaaa taatattccc
     3721 ccgcttgcag cgcccggcgg aaatgccaat cgctgaagag cgcaaatgcc agccggctgc
     3781 gtatcacgtc atcccgccgc agtattccag ccacccccca ccgcgaggtg cctttgggaa
     3841 catcaacgaa gacttgttgg ggtcggtggc cgcggtaggg ccggctaatc tgagaatcca
     3901 gccacgccgc gcccgcccct aatgcggaaa tcgccagcag ggccagcaag ccaagtctcc
     3961 gcattccgcg ttagtccttc tccacggaac cgctgcgtcc gtgatcccgt ttctgcccta
     4021 ggtaatcgcg cagaagaatc gcagcggcta cgtcgtcaag ggggtcttcc ctggggcgcc
     4081 cgctcctgat ttccgtcgcc atctgccgcg cctcccagct cgtcagccgc tcgtcccgca
     4141 tttccacttt aatgttcacg gcctttccca accggcgagc gaagcccaga gcctccttcg
     4201 ccatcgcacc ggcctcgccg gtcaaatgca gcggatgccc cacgataatg cggcagacgc
     4261 catttcggcg gcatatttcc cgcaggcgtc tcatatcgtt ttggcgattc gtgcgctgca
     4321 tcgtcgtcaa gggccgagcc gtcaggccga gttcatcgga tagtgctagc ccgatccgct
     4381 tccggccata atccaccgcc agaatcctcc gaggctcacc ctcgctcttt ttccttcctg
     4441 tcgtatttcc gccgcttgga attgtccgga tcagctgatc ttcccattat acgcgaggcg
     4501 caccgttcaa aatccggccc ggccggtaac tcttccctcg aacctcgcgc ataagttaga
     4561 tagatttcga cgcaccggag tcactgcttg cggccgcgct cgccgctctc ccacgctggc
     4621 cagaatcccc tgttcttcat ctcctggccc agtcccgttt ccttcagcag ctgctcgatc
     4681 tcggcgcgcg attgccgccc tacaatcggc tcgctcgtgt ccgcaagcgc atacgcggtt
     4741 attgcggcaa tccccgcctg cctcttgagc gccgcaatat ttgccttgtc gaacacatcc
     4801 gatgtggcgt gttcgttcac aggtttttga tttggatcga gcgtcaaaat gccttcaagg
     4861 atgaaatcca atctatccgc atcaattcgc gccccgagag tgaaatcctt cacgcccatc
     4921 ggcgcgagtg gcgcaagcgc ttcgcgcacg ggcgccagcg catccttgcg gcccgccaat
     4981 gaatatccac tgatagcccc aatcccgcca tcaaaaagaa tcgccgcgct cgtccgctcg
     5041 agctcggcgt gatgcgctcg tgtataagcc cacgctcctg gcgtcccttt ctttccgtcg
     5101 gcgaacagca cgaaacggat cgaccgcctg ggcaccgatc cggaggaatg aattacgcgc
     5161 gccgcatcga tcactagcgc cgtatcggga ccgtcgctga ggtgcgcccc gagcaccaca
     5221 aattcgtctg gatcttcccg tccgcgaatt tccgccacca cgttctcgga ccccgccgcg
     5281 ccggccaccc cctcagcttg tccgtcgctt cgtccgtccg gagtcgtgaa cttttccgtg
     5341 cgcacttcat cgacgcccgc ttggcgaaac gcttccaccg cccagccggc cgctcgcttg
     5401 gctccaggcg aacttttcgt tcctctaccg gcgcggctcg tcagatcgcg cagatttttt
     5461 tccagcggcg atgcgccgag cgcgctcttc atcatcgcgg gcgctaaatc cgcgcacgat
     5521 tttcccactg cgcacgtacc ggctccattc ggcaccttcc acccgcttgg tggattgatg
     5581 ggagaaactt gcgccgggca aatcgaggca aacagcagca tcactccagc cagtactact
     5641 gggacccatc ttttttgctc cacgcgcttc ggtcttatag acattgcacg aaacccgacg
     5701 tactctctgc cttcgatctt cgcagtcatc gtctttgtca tgccgcgtgg agatgatagc
     5761 gaacactgcg ccacgcggcc acatcgattg catcagagga ttttcctgtg gaaaagacgt
     5821 acctcttcgg gaataaagtg cccactacgg gaaacttcta cacagaatac agtgttcgct
     5881 gtattgggct tttcacgcat ctctttggca ccgcgcaagt tacgaatatg gacaaagatt
     5941 ggcaatgtag ctgccaacca gatcatcacc agtccgcgtc ggaaaaagcg cgttacttcc
     6001 tcggaggttt gcgtagttgc atttcccccc tcctgggaat cacggcagtt cccgtttggt
     6061 aacatcacag tcgtttcgtt gagaaaataa aaaattcccc tcgttaaact gttttggcga
     6121 gggacgggaa ggttctattg aagaaagtgc gtgacgtatc gaaactccta accaacaaac
     6181 ggaaactcga acgaaaccaa tccaaccact tcgtattctg tgaaaaacga agcggcctgg
     6241 cacaattcca cggcgcctgc gagttggaca gggactccac catcgagcgc atggccagca
     6301 cgcttgccat gcaatgcctg gttcgtggtt ccgaaccgga cgatttcgcg attctggtcc
     6361 ccgtggacag catacttgca ggtcggctgg agtcgcgcgc caaggaactc cttgacgaag
     6421 gtcgctccat agccaatccc gcttcgctca gtccccgtca gcaggaaatt ctgcattcga
     6481 tcctctgcaa tcacgccaac aaggagattg ccaacaagct caacatcact gtgcgcaccg
     6541 tgaaattcca catttccacg ctactcacaa aattcggcgc ggaaaaccgc tcggagttgg
     6601 cgcgtagggc ttcgggattc ctgcgcacct ccatgcatgt cgaagatggc ttcccgaatc
     6661 catccccggg cgttagacgc gacgatttgc ggccaattcc cgttgattct caagatcgcg
     6721 cgcggccgaa agcagggaat ctccgcattt cacagcagcg gatcctcacc gcatagtagc
     6781 cacgaacctc tccgccgccc cgaacccagc ccagggcgcc ggcctcgtag gtttctcgcg
     6841 gaatctccag cctctagtct ccagaacgtc agcgccagcc gagccctggg gcaacagtcg
     6901 tcagaatcgc ctccatggca tgcgaattgt agtcaacacc cagttggctg ggaacggtca
     6961 gcaatagcgt gtcggcttcc gcgatcgcct cgtcttttct aagctgctcg atcaggacat
     7021 ccggctcggc agcatagcta cggccgaaga tcgcccgtgt gggttcgccg ctaaggaagc
     7081 caatctggtc cacttcctca cctcctcgcc cgaaataggc gcgatcgcgg tcgtcaacca
     7141 gtgcgaagat actgcggctg acggaaacgc gcggagtgcg agtgtggcca gcctccttcc
     7201 acgccgcgcg aaaggcgcga atctgggctg cttgctgtac gtgaaaaggt tcaccggtct
     7261 cgtcaaattt cagggttgag gattgcaagt tcattccgag ctttgcggcc caggcggccg
     7321 tggcattggt agctgcgccc caccagatgc gatcgcgcaa tccttccgag tacggctcaa
     7381 tacgcagcaa cccgggcgga ttaggaaaca tcggacgcgg gttcggcttg gcgaagccct
     7441 tgccgcggag tagctccaaa aactcttgtg cgtggcgccg gcccatgtct tcatcgttct
     7501 cgccttcagc cggctggtat ccgaaatgac gccatccatc gatcacctgc tcgggtgaac
     7561 cgcggctgat tcccaattgt aatcgcccgc cggcgatcag atccgcggcg ccggcgtcct
     7621 ccgccatgta ataggggttc tcataccgca tgtcgatcac tgcagtgcca atttcaatcc
     7681 ttcgcgtctt agccccgacc gccgcgagca atggaaacgg tgatgccaat tgccgcgcaa
     7741 agtgatgaac gcgaaagtaa gctccgtcca ttcccaggcg ctcggcctcc accgcgaggt
     7801 cgatggattg cagcagtgcg tcagacgccg accgtgttgc tgattgcggc gcagccgtcc
     7861 agtggccaaa tgagagaaaa ccaattttct tcatgcaggt cagtcctgtc ttctgtcctt
     7921 gatcgcggcg ctatgctcgg taagcccgcc gacggtcttt ccctagccgt cgtaggggca
     7981 cagcatgctg tgcccggcaa acatgcttgg ctagttgtcg cccatccgct cccatgcacg
     8041 ggcgtgaagt ctcttccacg ccaaaaggcg caaacggtag cgttttcttg ttgccagcgc
     8101 cggaggcgcg attgtattta gcccggcacg cagcgaagcg gagtgccggc tcgccctgag
     8161 gaacgaaggg gatacggaaa cgaaaattga aaagtcccgg agggacgatt gaacctgcta
     8221 tcgacttttt ctgcggcctc gcaatcgccg cagcaaaatg gatgggaggc acgagttact
     8281 tcggctcagg gagtgcgcgc cggccgcggc ggcaaggtcc cgccttcggc aagaatcttc
     8341 atatttttga gcccgttcat gaacaacgtt cttctcctcg cgatgtcgcc ctctcgtgct
     8401 gcgtcgtcag cgagcatgga gttatcgaaa accagcgtgt acaccagctt ggaagtcgtc
     8461 gccgtcactg gccgcgcttc aagcgtgccg tgatacagat tgtacggttg acctacacga
     8521 accggctgag tgtacgtgta agaaagctcc gttttgccga ccatcacttc gttgccgatc
     8581 gatcgaacga ctccaacctc tccatccgtt cccgaaagaa ttgtgcacgg cacctgaaac
     8641 cattcgccga tgtcgcaaaa tttgcccacg cgtttccaca cctctgccgc cggccgattc
     8701 acgtccatct ccatcttgat tgatacataa tgcggattcg gaatcgcgag cggtgccggc
     8761 gcggggcgag ctgcggctgg cgctggcgac ggcgcttggt ttgttgcttg acccgaagct
     8821 tgtccctgcg cgtaaaacga gaggcaaagt aacagggcag cgaatgttgc ggtcatcagt
     8881 cgattcatcg gtggctcctt cggcgcaaat cgttgcacac cttgatgagc caatcaagac
     8941 gcgcaatgcg agtttccgcc gtctct
//
LOCUS       DATNDT010000372         2804 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_377477, whole genome shotgun sequence.
ACCESSION   DATNDT010000372 DATNDT010000000
VERSION     DATNDT010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2804
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377477"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /locus_tag="VN788_11955"
     CDS             complement(<1..494)
                     /locus_tag="VN788_11955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXU21287.1"
                     /translation="MLSIVIPIHNEEPSILPLYDRLTSVLDQLQKPFEIIFVDDASTD
                     RSFDLLANLVETDNRLKVIRLRRNFGQTAALSAGFDEAQGDVVVSLDGDLQHDPEDIP
                     ALLAKIDEGYDIASGWRKNRVDNAITRKIPSRIANWLMAKASGIELRDFGTTFKAYRA
                     EVLK"
     gene            565..1443
                     /locus_tag="VN788_11960"
     CDS             565..1443
                     /locus_tag="VN788_11960"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HXU21288.1"
                     /translation="MQSSIDRRSNRPAILLVDDNRDLLRFLERLMSESGWTLLTAESA
                     TEAKQLFEKNRPDAALLDYMLPDGNGVELALEFHEKKTDLLAIVMSGAILPPEEEALC
                     EEHGFPVLRKPFLASDVMNQIRRRLELHSQAEVKFTSRALKVFFSYSHRDEKLRDRLD
                     AHLSSLKHMDLIHSWHDRKIAAGSDFNEAIDRYLDNADLVLLLVSPDFLNSDYCYRKE
                     MKRALERHAVGEAQVVPVILRPVDWEKAPFAHLQAVPKDGRPITRWANRDEGFLDAAK
                     SIRRVAEELSATLRRS"
     gene            complement(1457..>2804)
                     /locus_tag="VN788_11965"
     CDS             complement(1457..>2804)
                     /locus_tag="VN788_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HXU21289.1"
                     /translation="AAAEGVRLIVSVDTGIRAGDVVRAARELGIDVIITDHHLPEAAL
                     PPAVAVLNPNRPDCQYPNKNLCGAGVAFKLAQGLFETLGWSDGKLRRMKESFLRLAAI
                     ATVSDVTPLTGENRVIVKHGLRGLGNTRNPGLRALLAVSGFAPGETPTATQIAFRIGP
                     RINAAGRMATATDVIELLLSADETRAREIAGRLQALNQERQETEAGIVRLILRECETA
                     PVTEEQSALVFSGAGWHCGVVGIVASRLVERFCRPVFVISEDAETGEAHGSGRSAAGF
                     HLLEALESMPEVFTKFGGHRQAAGVTIPTGRIEEFRTRLGVYARARLSPEDFRPLVEF
                     DSEMDLAEATGETAEEILSLAPFGFGNPIPVFHVCAAELAGPPSILKEKHVRLNVRSN
                     GKSLSASAWNFAARAADMRAGDLLDLAISFEDDPYGRSRGWPGWTVTLRDARRSRI"
BASE COUNT          584 a          826 c          809 g          585 t
ORIGIN      
        1 tctttcagca cttccgcgcg atatgccttg aatgtagtgc cgaaatcgcg caactcgatt
       61 ccggacgctt tggccatcag ccagttcgcg atgcgcgatg gaatcttgcg ggtgatcgcg
      121 ttgtccacac ggttcttccg ccatccgctc gcgatgtcat acccttcgtc gatcttcgcg
      181 agaagcgccg gaatatcttc gggatcgtgt tgcagatcgc cgtcgagcga aacgacgaca
      241 tcgccttgcg cctcgtcaaa accggcggaa agcgcggccg tctgaccgaa gttacggcgg
      301 agacgaatca ctttcaggcg gttgtccgtt tcgacaaggt tcgccaacag atcgaagctg
      361 cgatccgtcg acgcgtcatc gacgaaaatg atctcgaacg gtttctgaag ttgatcgagg
      421 accgaagtca acctgtcata gagcgggaga atcgacggct cttcattatg gatgggaata
      481 acgatcgaca gcatggacac cctttctctt agtgtagcaa gggctttctc actgacccga
      541 tcgttcctat aatgtaatgc ggtcatgcag tcttccattg acaggcgctc aaatcggccg
      601 gcgatcctcc tcgtggacga taatcgcgat ctactgcggt ttttggaacg tctgatgagc
      661 gaatccggat ggaccctcct gacggcggaa tcggcgaccg aggcaaaaca gctctttgaa
      721 aagaataggc cggatgccgc gctgcttgac tatatgttgc cggacggcaa cggcgtggaa
      781 ctggccttgg aattccatga gaaaaagaca gacctgctgg ccatcgtcat gagtggcgcc
      841 attttgccgc cggaagagga ggcgctttgc gaggaacatg ggtttccagt actgcggaaa
      901 ccgtttctgg catccgacgt gatgaaccag atccgtcgcc ggttggaact gcattcccag
      961 gccgaggtca agtttaccag cagagctttg aaggtatttt tctcttactc gcatcgagac
     1021 gaaaagctaa gggaccggct ggatgcgcat ctgagcagtc tgaaacacat ggatttaatt
     1081 catagctggc atgaccggaa gatcgccgct ggaagcgact tcaacgaagc gatcgaccgg
     1141 tatcttgaca acgccgatct ggtactgctt cttgttagtc ccgattttct aaattccgat
     1201 tactgttacc gcaaagagat gaaacgggcg ttggaacggc atgccgttgg cgaggcgcag
     1261 gtcgtccctg tcatcctgcg gccggtcgat tgggaaaagg cgccgtttgc acatcttcaa
     1321 gccgtgccga aagatggcag gccgattacg cgatgggcta atcgagatga aggctttctg
     1381 gacgcggcaa aaagcattcg cagagtggcc gaagagttat cggcgactct gcgtaggagc
     1441 taggttcgaa gccggattat attcgcgacc ggcgagcgtc gcgtagggtt acagtccatc
     1501 cgggccagcc ccggctccgg ccgtaagggt cgtcctcgaa cgagattgcg aggtcgagaa
     1561 gatcgccagc gcgcatatcg gccgcgcgcg ccgcaaagtt ccaagcggaa gcgctcaggc
     1621 ttttcccgtt gctccgcacg ttcagacgga catgcttctc tttgaggatt gatggtggcc
     1681 ccgcaagctc ggctgcacac acatgaaaaa cgggaatggg atttccgaaa ccgaatggtg
     1741 cgagcgagag aatctcttcg gccgtctcgc cggtcgcttc tgccagatcc atctcgctgt
     1801 caaactccac cagagggcgg aagtcttccg gcgacaatcg cgctctcgca tagacgccga
     1861 gccgagtccg aaattcttca atgcgcccgg ttggaatcgt gacaccggcg gcttggcgat
     1921 ggcccccaaa ttttgtgaac acctccggca tcgactccaa ggcttcgagc agatgaaaac
     1981 ccgcggcgct tcggccggaa ccatgtgcct cgccagtttc ggcatcctca ctaatgacga
     2041 agacgggccg gcaaaatcgc tctaccagac ggctggcaac gatgcccacg accccgcaat
     2101 gccatccggc gccggaaaac acaagcgccg attgttcttc cgttactggc gccgtctcgc
     2161 actcccgcag gattaagcgc acaataccgg cctcggtttc ctggcgctcc tggttaagcg
     2221 cctgtagccg cccggcaatc tctctcgccc tcgtttcatc cgcgctgagc agcagttcga
     2281 tgacatcggt tgccgtggcc atccggcccg cggcgttaat ccgcggaccg atgcggaacg
     2341 caatttgcgt ggccgtaggg gtctcgcccg gcgcaaatcc cgacaccgcg agtagcgcgc
     2401 gcagccccgg gttgcgcgta tttcccaaac cacgcaagcc gtgttttacg atgacgcggt
     2461 tttcgcccgt gagcggcgtg acgtcggaca ctgtcgcgat cgccgctagc ctgaggaatg
     2521 actccttcat gcggcgcagt ttgccgtcgc tccaaccgag cgtttcgaat aagccttgtg
     2581 caagtttgaa cgcgacgccc gcgccgcaga gattcttgtt tggatactgg caatctgggc
     2641 gattcggatt cagcaccgcc acggccggcg ggagcgccgc ctccggcagg tgatggtcgg
     2701 tgataatcac gtctattccc agttcccgcg cggcccgaac cacgtccccg gcgcgaatgc
     2761 ccgtatccac gctgacgatc agacgaacgc cttccgctgc cgcc
//
LOCUS       DATNDT010000373         7465 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2522191, whole genome shotgun sequence.
ACCESSION   DATNDT010000373 DATNDT010000000
VERSION     DATNDT010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7465
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2522191"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="VN788_11970"
     CDS             <1..402
                     /locus_tag="VN788_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HXU21290.1"
                     /translation="AVGSSLLRTLLRSDVRSLEAMVMADKTSQNALSKLHFEAGPAHV
                     IAEIEALLRQLHAKAVLNVPDPLRSARLFAALFKGSDLLMIARFDQAKAEDDDEIESY
                     CLSAVAMFIAAHRGNDHAGGYLPSSRSKNKI"
     gene            complement(874..2232)
                     /locus_tag="VN788_11975"
     CDS             complement(874..2232)
                     /locus_tag="VN788_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21291.1"
                     /translation="MSGNPQSPPGAVLPLPTRPPKGPVIPVPVTHGQPGLATPSIATP
                     GPSSYPPAFFLQPGGTYFVTSYNAARGGYVLPGGVVIPPNEPHLVTANNWPLGSALVW
                     GDKGGSGQVTASDSTTMAIVNQIIAGRQNPTTIRSPSPATPAGSPAAVLPNHGASTQG
                     NTGVTNAASRITHEDTGQSNLTDKGPDRASGSTGNQLTQNSVTAEPGSTIQPAAKIVE
                     ETQSTAQPASQMKTTAMATARETTTVQTCNLCDPWTLTDGHVVSVTSNGETYTGLLLT
                     NNGSFVPFASLPVWSGSTPLNTFTGLVRTNLGYIYYVNGQNTVFGPGSINGGFYANGP
                     GTQIAVAPPTPPSTMTNPTSPAQTANPGAQTPPLANGANGPNWWFVGLSRGMEATANG
                     NELGREKLRSKWIGGEIGYMAGQILFYGFVAPRIYQNQKSGDNDAAAKTPQQNGEPTR
                     LP"
     gene            2222..2524
                     /locus_tag="VN788_11980"
     CDS             2222..2524
                     /locus_tag="VN788_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21292.1"
                     /translation="MPLTGGSLDGPILGRTEVPGGASDGGRTATGRLPEAGGGNTGLA
                     AGVGLEGMTLGPPEPMLAPPSDTVGSGLVLCGGPATGGNGAGGWEGVGSVGLGTLG"
     gene            2992..3264
                     /locus_tag="VN788_11985"
     CDS             2992..3264
                     /locus_tag="VN788_11985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21293.1"
                     /translation="MRRSNMNLWGRYVYLRGIGLNVRTRHMHQRSSHVDFRRYNVHLR
                     GAHMDFGGRHMHMRHRDMRHHLGTSLVQKAEEPPKRSKDEEQNHHQ"
     gene            complement(3520..4506)
                     /locus_tag="VN788_11990"
     CDS             complement(3520..4506)
                     /locus_tag="VN788_11990"
                     /inference="COORDINATES: protein motif:HMM:NF015737.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HXU21294.1"
                     /translation="MSFSSIQTMLEGRRDQLQSPHLTAAPLGYANGEALGYSSTGRAR
                     NAFASIPFKASPSVAAAGPSWGSWAEGLGNWQRNSAVAAGDLDHFAETYGAQGGVDAT
                     WQSLSAGDDALVVGLVASWMTSRVTYDGSPVSLQLKGPGVGLYGTYLRGNFSTDLAAK
                     TDFLTMDEAFSGLSPNASISATNFGVTGNAQYKIMTGTASFVEPTGGFSFTRTDFGGG
                     AAALDLKDASTLRLQAGLRFGTSTKIADTAVEPTLRVLAYDNVVAEGSSTAADTPGAA
                     IVPTDQGKVRGEIDPAVNFDFGNGYSSNISGQVVFGEDLVGGALTAQLRKQW"
     gene            complement(5258..5344)
                     /locus_tag="VN788_11995"
                     /pseudo
     CDS             complement(5258..5344)
                     /locus_tag="VN788_11995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
     gene            5784..7046
                     /locus_tag="VN788_12000"
     CDS             5784..7046
                     /locus_tag="VN788_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXU21295.1"
                     /translation="MKSSIRAFIFSCCLAFVLVPFASQARGPYGSITVGNWKGGAYTD
                     DKSGAFSNCAASAVYQSGIVFLVSVGQDFSWTLGFAHENWKLTPGEAFALALTFDGQQ
                     AIHVQGLPIGNNLVKVSMPANSTLIGQFRKAKMMTAFAQGQLFQFDLKQTAQLLPSLA
                     NCVAVVKKNGLANAGEFAVALTKPAAAAADQSSKPAKTFTQSGTGFAISAGGHVVTNA
                     HVVHGCVGDIQGNLGGEAPLKLRLVSSDRTNDLALLQAPSPFKEIATIRDKPVHSGDS
                     VVAIGYPFHGMLTSDFTVTTGIVSSLSGILNDTRYLQISAAVQPGNSGGPLLDTDGQI
                     IGMVAAKLDALKIARATGEIPENINFAIKTGMLRDFLDNSAVTYQTAAETKGELKTSD
                     IAKNARGFTLLISCTAEEKTASAKKGEE"
BASE COUNT         1596 a         2164 c         2174 g         1531 t
ORIGIN      
        1 gcggtgggat catcactgct tcgcacgctg ttgagatcag acgtccgcag cctcgaagcc
       61 atggtcatgg ccgacaagac gagtcaaaac gcgttaagca agctccattt cgaagccggc
      121 cccgcccatg tcatcgccga aatcgaggcc ctgttgcgtc agttgcacgc gaaggcggtt
      181 ctgaacgtgc ccgatcctct ccggtccgcc cgcttgttcg ccgcgctttt caaaggatcc
      241 gatctcctga tgatcgcacg cttcgatcag gcgaaagcag aggacgacga cgaaatcgaa
      301 tcctattgcc tgtcggccgt cgccatgttc atcgccgcgc accgtggcaa cgaccacgcg
      361 ggcggatatt tgccttcctc aaggtcaaaa aacaaaattt gaatcgtgcg tgaccgacgt
      421 tgtcaaaata accgaaaccg gtttgaaaat catggaggct gaagagcttt cgattggcga
      481 caagatcgcg gaaaaatacg cttgttgaac tatcggttgc caccctgata ctcgttctca
      541 ctaatttcta cgccattcta ctcaaaggct tgaggcgagc gggacgcaag gataggcaat
      601 taacggtagc gagaaccgta ggtacccacg tgctgcgatg gaaaaccgcc tataaatcaa
      661 tggggattga ctgacactct ctccgccagc gctcaaaaaa atccacgcta cgacgtgacc
      721 tgagagcgtt cgtattttgc gagcagttcc acgaccgtca cggacatgga cacgacgatg
      781 gccggggatt tcccaccaag cctctacatg gagggtgagc gggatcgcgt ctgtccactc
      841 gtggacgcca tgccccggca catccaatat caatcaagga agacgcgtcg gctcgccatt
      901 ttgctgcggc gtcttcgcgg cggcgtcatt gtcgccgctt ttttgatttt gatatattcg
      961 cggtgcgaca aaaccataaa aaagaatttg accagccatg tatccgattt caccaccgat
     1021 ccacttggac ctaagcttct ctcgcccgag ctcatttccg ttcgccgttg cttccatccc
     1081 cctgcttagg cctacgaacc accaatttgg gccattcgcg ccatttgcca acggtggcgt
     1141 ttgagctcca ggatttgcag tttgcgccgg agaagtcggg ttagtcattg tggagggcgg
     1201 ggtcggaggc gctaccgcaa tttgcgtacc gggaccattt gcataaaagc ctccgttgat
     1261 cgagcccggt ccgaacaccg tattttgccc gttgacataa tagatgtaac caaggttggt
     1321 tctcaccaag cctgtgaagg tgttcagcgg ggtcgaacca gaccacaccg gcagagaagc
     1381 aaatggaacg aaggagccgt tattcgttag aagaagtccc gtatacgtct cgccattcga
     1441 cgttacgctg acgacatgcc catctgtcaa agtccaggga tcgcagagat tgcacgtttg
     1501 gaccgtggtc gtttccctgg ccgtcgccat cgcagtggtt ttcatttgtg aggcaggctg
     1561 agccgtcgat tgcgtctcct cgacgatctt tgccgcgggc tggatggtag aaccgggttc
     1621 tgctgtaact gaattctgtg ttagttgatt tccagtgctg ccgctcgccc tgtcagggcc
     1681 cttgtcagtc agattggatt gaccggtatc ttcatgcgta atcctggaag cggcgtttgt
     1741 tactcctgta ttcccttgcg tgctggcccc atggttcggc aaaacggcgg caggcgatcc
     1801 agccggtgtt gcgggtgagg ggcttcgaat cgtggttggg ttttgccgac cggcaataat
     1861 ctgatttacg atcgccatcg tcgtcgagtc agaggccgtg acctgacccg aaccaccttt
     1921 gtctccccac acaagagcgc tcccgagagg ccaattgtta gccgttacga ggtggggttc
     1981 gtttggcgga atcacgaccc cgcccgggag gacgtacccg cccctcgccg cattataaga
     2041 agtgacaaaa tatgttccgc ctggctgaag aaaaaacgca ggcggataac ttgatggccc
     2101 gggtgttgcg attgaaggcg ttgcgaggcc gggctgccca tgcgtcactg gtaccggaat
     2161 aaccggcccc ttgggcggcc ttgtgggtaa cggcagaaca gctccgggag gcgattgagg
     2221 attgccgctc accggcggca gccttgacgg ccctatatta ggccgcaccg aagttcccgg
     2281 aggagccagc gatggcggta gaacagctac gggaaggcta ccggaggcgg gaggtggcaa
     2341 tacgggattg gctgccggcg tgggattaga gggaatgacg ctagggccgc cagaacccat
     2401 gttggcgccg ccatctgaca ccgtcggttc tggattggta ttgtgtggcg ggccagcaac
     2461 tggcggcaat ggtgccggcg ggtgggaagg tgtcggcagc gtgggattgg gtacgctcgg
     2521 ttgagaatca aacacaacaa acaaagtaca ggacccgcca ccacagcctg ttgtattgga
     2581 attcaaataa tgcatccctg ctggcggcgg gggcggattg ttgcctgacc acttgaagtt
     2641 tacttcgtca aaacgagtgc ccgacccatc gaccccctca tccacgatag atgtgcattg
     2701 ttgaaccgga cacccgcttg gcgcggaggc gggcgaaaga gaagatttcc gggtggcgac
     2761 actgcccgcg ggacttgtct tttgcgcaac ggaggatgga ccggagttct tcgcgttgag
     2821 gggcgttatg gtccggccga ctttcgctcc ggtgtgaccg gacgcattta agtcgcgact
     2881 gcttgcctta gtctggagac cggccatgcc ggcgccagga gagttggctc cgatcttcgg
     2941 ggagtgagcg cggactcccc ccgcctgtat atgaatcccg ggagcgtgca ggtgcggcgt
     3001 tccaacatga atctttgggg acgctatgtg tacctgcgtg gaattgggct gaatgtgcgc
     3061 acccgccaca tgcaccagcg gagttcccat gtggattttc ggcggtacaa cgtgcacctg
     3121 aggggggcgc acatggattt tgggggccgc cacatgcaca tgcggcaccg ggatatgagg
     3181 caccacctgg gcactagcct cgttcaaaaa gcagaggagc cccccaagcg ttccaaagat
     3241 gaagagcaaa atcatcatca gtaggtctcc agctttccga agggcatcgt tctgttcaaa
     3301 tcctcaattt agctgaacgc gaacctctac ccctgttccg caccgcctca atatcgcggg
     3361 cctttcgaag gagagaggcc atgctgagcc tcacatccag caatacttcg gctcaaaaca
     3421 gcggggttgg aaatcgactg cccgacagcc ggaatcgttg cctttgagcg caagtcggcg
     3481 catctctttc ttctactcgc gctcaggatc aaggcctcgt taccattgtt tcctcaactg
     3541 agcagtcaga gcgccgccta caaggtcctc accaaataca acttgtcccg agatgttgct
     3601 ggaatagcca tttccaaaat cgaaattgac ggccggatcg atctcgccgc gtaccttgcc
     3661 ttgatcagta ggcacaatag ccgctccggg cgtgtcggcg gcagtgctcg atccctcagc
     3721 aaccacattg tcataggcca ggacccttag agtcggttcg acggcggtat cagcaatctt
     3781 tgtcgacgtt ccaaatcgaa ggccggcctg cagccgcagc gtcgatgcgt ccttgaggtc
     3841 cagggctgcg gctccgccac caaagtcggt ccttgtaaat gagaagcctc cggtcggttc
     3901 cacgaatgaa gccgtgccgg tcatgatctt gtattgagcg ttaccggtca ctccgaagtt
     3961 cgtggcgctg atcgaggcat tgggcgaaag accgctgaaa gcctcgtcca tagtcagaaa
     4021 atcggtcttg gcggcgagat cggtcgaaaa attgccgcgc aggtaagtcc catagagacc
     4081 gacgccgggc cccttcaact gaagactaac aggactgcca tcgtaagtga cacgcgaagt
     4141 catccagctt gcaacgaggc ccacgacgag cgcatcgtct ccggcggaca gactctgcca
     4201 ggtggcgtca acaccgccct gggcaccgta agtctctgcg aaatgatcca agtcgccggc
     4261 agcgacggca gaatttcgct gccagtttcc aagcccctca gcccaggaac cccaactcgg
     4321 gccggccgca gcaacgctgg gggacgcttt gaagggtatg gaagcgaagg cgttgcgagc
     4381 gcgtccggtg gacgaataac ccagggcctc tccgttcgcg tagccgagcg gcgcggccgt
     4441 gaggtgcggg ctctgcaact ggtcgcgtct gccttcgagc atggtctgaa tactggagaa
     4501 ggacaattgt tgctgcgaga ccgcaacgct tcgcacactc tgactaatcg aggacgaacc
     4561 tgtgcaaact gccggaacaa ctactgcacc ggcgcccgcc agaacgcatt ggctaccgga
     4621 aattattgct ccggcaggac aactgcaccc agccgcaagc tgggcatggg cactaccagc
     4681 cacgagtgac gcaagcagaa atacaaaaga tgcaaaaaat ctcctataac cccgcacttt
     4741 agctccctct caaacttcga attctaaacg agcctcaaaa cgacggcatc aggtaaggtc
     4801 gcacgtttcc gttgcagaac cgcaagtaga tcaatttcat acgctggcac cgtctacagg
     4861 cgcgacgcga aggtgcaaca agtgtcgtct gatagcatca cccaaatggg tcatccgggc
     4921 gtttcgcttg aagcggtcat caccaaggga tggcgttggg gcagatgcag gtaataggcc
     4981 tttggaattg cctgaagccg atcatcaccg cgcgcagcaa atgaactcgc cgtctctttt
     5041 ggccgtgtat ttgtttgccc aatacgggac gggccgtcat ccgcgccggc gatgtcgcgt
     5101 tctcaaggcc attcctccga ctacctgttg gagccaaaat tgatgtcaaa gtcgccatgt
     5161 ttaattctcg caagcatggc gtggaaatca gataggtcgc gaggacaatg gaaaaacagt
     5221 acccgtcgat ttcccggtct ccaattcggc cgcgcgtcag atgcgtgtag gccgtccgca
     5281 gccatggcac cgcaattttt gcggtgtccg cgcgcgcaat caggtcgcca atcacacgat
     5341 ctgccagttt cctgcgagcg ggaagatttc catctcaaac gtcaggtgat cgcacgccaa
     5401 accggatcgt agttgcgcaa agtgccgttc ccggccaggc gaagttcaaa catgacgaga
     5461 gtcatcatgc cggttgataa ggacgtcggc ccgcttcaaa ccacccgatt cccgacaagc
     5521 atcaggtagg gggtgtaggt cacgccgcgc cgggcagcgg cgacgcccgc cttccaggcg
     5581 ttggaaacgt gccgcctggc ggtagctcga atggcgggtt ttggttcgaa aagtcgaaaa
     5641 tgctttatct ttcaacgggg gttgactgac actcactccg ccacggcccc ctgaaaactc
     5701 catagaccca ggatcgctgc cgaagtgctt gcagaccgat ttcattgcca cttatgatgg
     5761 taacgtcatc gtgagtagca agaatgaagt cctccatcag agcgttcatt ttctcctgtt
     5821 gccttgcctt cgtgctggtg cccttcgcgt cgcaggcccg tggtccgtat ggctcgatca
     5881 ccgtgggcaa ctggaagggc ggcgcctata ccgacgacaa gagcggcgcg ttttcgaatt
     5941 gcgcggcctc ggccgtttac cagagcggca tcgtcttcct ggtttcggtc gggcaggatt
     6001 tcagctggac gctcggattc gcgcacgaga actggaagct gactccgggc gaggccttcg
     6061 cactcgcgct gacgttcgac ggacagcagg ccatccacgt gcaagggctg ccgatcggca
     6121 acaatctggt caaggtcagc atgcctgcga attcgacgtt gataggtcag ttccgcaaag
     6181 ccaagatgat gacggccttc gcccaaggcc agctgttcca gttcgacctc aaacagaccg
     6241 cgcagctcct gccatcgctc gccaattgcg ttgccgtcgt gaagaagaac ggtctggcca
     6301 atgcgggtga gtttgcggtt gcgctcacga agccggccgc cgctgccgca gatcagtcgt
     6361 cgaagccggc caaaaccttt actcagagcg gcaccggttt tgcgatcagc gccggtggcc
     6421 atgtcgttac caatgcgcat gtggtccatg gctgcgtcgg cgacatccag ggcaatctgg
     6481 gcggcgaagc gccgttaaaa cttcgtctcg tttcgagcga ccggaccaac gacctcgcgt
     6541 tgctgcaggc ccccagcccg ttcaaggaga tcgccaccat ccgcgataag ccggtgcatt
     6601 ccggcgacag cgtggtggcg atcggctatc ccttccacgg tatgctgacc tccgacttca
     6661 cggtgacgac gggtatcgtc agttcgctga gcggcatcct gaacgacacc cgctatttgc
     6721 agatcagcgc cgccgtgcag cccggcaaca gcggcgggcc gctgcttgat accgatggcc
     6781 agatcatcgg catggtcgcg gcgaaactgg acgcgctgaa aatcgccagg gcaaccggcg
     6841 aaatccccga aaacatcaac ttcgccatca aaacgggaat gctgcgagat ttcctcgaca
     6901 atagcgcggt cacctaccag accgcggcag agaccaaggg cgaactgaag acctccgaca
     6961 tcgcgaaaaa cgcgcggggc tttacgctgc tgatctcgtg cacggcggag gaaaagaccg
     7021 cgagcgcgaa gaagggcgag gagtaggggg tgttgcggcg cgagaatggc caagtgcccc
     7081 gacgccgtca tcacctccgg cggatagcaa gccacgccac ccattccgag agatgccgtt
     7141 tacttgcgga tgacatctcg ttctacgccg cgcacaaggg ccgacttgaa tgatctgccg
     7201 ggggccccgc gctctgcctg cgcatcgatc gccaatgttc gggagtcttg tccggtcccg
     7261 atcgaagcca gaccgggatc cgggtttctg tcgtgcctta tcgacgcgag ccggagtcag
     7321 gctcgcgcga aaccgcggtg gaacggtcga ccttttggaa atcgccttct ggaaattgtc
     7381 gtctagaaat tgaccgtcgc gctctggaac ttgtttccgg cgggcgcgcc gttcggatac
     7441 atgtcgaagc cgtaggtcgc gccga
//
LOCUS       DATNDT010000374         7591 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3255946, whole genome shotgun sequence.
ACCESSION   DATNDT010000374 DATNDT010000000
VERSION     DATNDT010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7591)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7591)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7591
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3255946"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VN788_12005"
     CDS             <1..271
                     /locus_tag="VN788_12005"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU21296.1"
                     /translation="QERAQYPAYEMYRVVMKNGKELLGTRVNEDSFTMQLRDESGNIH
                     SVQKFDVKEIERVADKSFMPSYKGQLTDEQLNDLVAYLASLGGAK"
     gene            268..1899
                     /locus_tag="VN788_12010"
     CDS             268..1899
                     /locus_tag="VN788_12010"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU21297.1"
                     /translation="MSTTKNLLCSLAVFALLLGLGFVCAAQDDINVTYDEILHAASHP
                     SEWLTYGGNYSSQRFSELKQINKQNVNTLKLRWVYQLRRTGIFESSPIVVDGVMYVTE
                     PPTTVTALDVRTGRPIWRWTADLPKHLLTIGLFPTNRGVAVLGDTVYVATIDAHLVAL
                     DAKTGAMKWSVEIGDNKKAVAITQAPLAINGKIIVGMGGGEGGLRGYIDAYDAKDGKR
                     LWRLYTIPTKGEPGVETWEGDSYKYGGATTWNTGAYDPELNTLYWGTGNPAPDWNGDM
                     RKGDNLYSCSLLAIDADTGKMKWYFQFSPHETHDWDSAEPPILFDAVINGKKRKIVAQ
                     ANRNAFYYVLDRETGKFLAGAAYAKETWAKGLDAKGRPIEMPNIDPTTQGNLIYPSIT
                     GSVNWTSPSYSPLTGLVYVDTREQGAYYFKGHPTMEPANPLDIGGGGGQRVVYGDDAY
                     SAIKALESTTGKIKWQFKMVGDSWTGTLATAGGLVFCADSQGNFFALDANDGKPLWNI
                     QLGNSVRANPITYAVDGKQYVEAAAGNAVSVFSLP"
     gene            complement(2038..2418)
                     /locus_tag="VN788_12015"
     CDS             complement(2038..2418)
                     /locus_tag="VN788_12015"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU21298.1"
                     /translation="MTIRAFCGLALLLALTAFAISPVSARNQDKRGEAELRTIHGSVV
                     DKNENPVPSSVVYLLNVKTQTVKTYIADDAGAYRFSGLDPNVDYEVHAEHKDMTSPNR
                     TVSSFDSRHDIDVTLKLSRKKQAR"
     gene            complement(2539..2778)
                     /locus_tag="VN788_12020"
     CDS             complement(2539..2778)
                     /locus_tag="VN788_12020"
                     /inference="COORDINATES: protein motif:HMM:NF044773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21299.1"
                     /translation="MTKCPECDAEIDLDEDEVEEGEIVGCPECDVELEVTQTHPVHLI
                     VLSEDEEEEEEDDETKDDDDEEEPEGEEEEDGDSL"
     gene            2867..3310
                     /locus_tag="VN788_12025"
     CDS             2867..3310
                     /locus_tag="VN788_12025"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HXU21300.1"
                     /translation="MPERREYPERPIIGVGGVVISDGRVLLARRGGLPLQGQWSIPGG
                     ILEVGETLIEGVQRELAEETGIEVRVRTLIEVFERIDRDSSGKLQYHFVVLDYLCEAV
                     RGTARAGSDVTEVSWAAPAELERYALTEIAGRVIRKAFELAGEGD"
     gene            complement(3327..4061)
                     /gene="uppS"
                     /locus_tag="VN788_12030"
     CDS             complement(3327..4061)
                     /gene="uppS"
                     /locus_tag="VN788_12030"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HXU21301.1"
                     /translation="MGNSAAKLIPDPKIHVAILLDGNGRWAASRGLPRAEGHRAGVTA
                     VRRIARAALSLGIGTLTLYAFSSHNWERPSGEVTSLLGLLEDYLRTEAHECAAQGIRL
                     RVIGRRDRIPATLVDAIEAAEHLTVAGRALELRIALDYSSREAILRASCWMMSSLEVS
                     DREFARRLGQVTHGGGSAPDVDLLIRTGGERRLSDFMLWECAYAELLFTPRMWPEFEA
                     ADLEAAVEDFLGRERRFGRLPEAAAS"
     gene            complement(4084..4464)
                     /locus_tag="VN788_12035"
     CDS             complement(4084..4464)
                     /locus_tag="VN788_12035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXU21302.1"
                     /translation="MVAKSGMLRLPKEERPKRSPQGALNAKVPAVSLDRLIHERTRLA
                     IVSALAVNASLTFKELKDFLRITDGNLSVHARKLEDAGYIGCNKSFSGRMPKTEYRLM
                     HAGRKALEKYLEHLEALIRATREG"
     gene            complement(4454..5020)
                     /locus_tag="VN788_12040"
     CDS             complement(4454..5020)
                     /locus_tag="VN788_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21303.1"
                     /translation="MDDRARDNIRFIRETMERASSFTAVPGWGGIAMGITAIGATAIA
                     SLQQSSTGWLITWACEALVAVAIATWTTCSKARDAGTAILSGPGRRFVYSFAPPLFVG
                     LLLTLVLVRAGLIGSIAGVWLLLYGTAVVTGGMLSIRIVPLMGLCFMVLGAVALFSPS
                     SWSNAFLAAGFGGLHIIFGAVIARKYGG"
     gene            complement(5174..5935)
                     /locus_tag="VN788_12045"
     CDS             complement(5174..5935)
                     /locus_tag="VN788_12045"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichyl-phosphate beta-glucosyltransferase"
                     /protein_id="HXU21304.1"
                     /translation="MNSGTPELSIIVPAYNEEARLERTLSRVHEYFRRDPRFANALEI
                     IVVDDGSTDGTARIAQEWERAAPNGGFRLFLNERNRGKGYSVRRGMLEARGRIALFTD
                     ADLSAPVEESEKLLAAISAGHDIAIGSRAIDRSLIAAHQSVFRETAGIIFNVLVRLFT
                     GLALHDTQCGFKAFLREPCRIIFEQQRIEGFGFDPEVLFLAQRHGLRTAEVPVRWAHD
                     PATKVHVAWDSVLMFGDLMYICWNRVFGRYPANRQ"
     gene            complement(5932..6633)
                     /locus_tag="VN788_12050"
     CDS             complement(5932..6633)
                     /locus_tag="VN788_12050"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013907759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HXU21305.1"
                     /translation="MNGEGKFVQGDTAEFEAARHEMVARQIRDRGIGDERVLNAMRAV
                     PRHLFVPPEYKSRAYSDEPLPIGEEQTISQPFMVAAMADALLLQGHERVLEIGGGSGY
                     QAAVLSQLALEVIAIEARPALAAAAADRIDRLGYRNVSLKIGDGSAGWPSCAPYAAIL
                     VTAAAPSVPQPLVDELAEGGRLLIPIGQDEHQELLRLVKKEGRIDRQSLYACRFVPLV
                     GRYGQRQDARETNRG"
     gene            6860..7537
                     /locus_tag="VN788_12055"
     CDS             6860..7537
                     /locus_tag="VN788_12055"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HXU21306.1"
                     /translation="MKTRTALIAVAFCIAVPVGAQTPMPGTQQKPAQHQPGTPAPHKP
                     GQPATDQTEKAEKPEPGTATAKPDAAKEAAIRHLMDLTQSSKLGDNIQTYITNQVRTG
                     LARALKPDALPKFMDEFSQKFVSAAPASSVTDAMVPIYAGAFSTEDIQGLIQFYESPL
                     GQRVVKNLPTVDRQSQEAGVRIEQAAALAILRDMSEEYPELKQILPPGTEQPTPGSET
                     PPAPPQR"
BASE COUNT         1724 a         2173 c         2177 g         1517 t
ORIGIN      
        1 tcaggaacgc gcgcaatacc ccgcatacga gatgtaccgc gtggtgatga agaacggaaa
       61 agagcttctt ggaacacgcg taaatgagga ctcgttcacg atgcagttgc gcgacgagag
      121 cggaaacatc cactccgttc aaaagttcga tgtgaaggaa attgagcgag tggcggataa
      181 gagcttcatg cccagctata agggccaact tacagacgag cagttgaatg atctggtggc
      241 ctacctcgcc agcctgggag gggcaaagtg agtacgacga agaatcttct ttgcagtttg
      301 gctgtgttcg ccctgctgct gggcttagga ttcgtatgcg cggcgcagga tgacattaac
      361 gtaacttacg acgagatttt gcacgccgcc agccacccaa gcgaatggtt gacctatggc
      421 ggcaattatt cctcacaacg cttttcagag ctgaagcaga tcaacaagca gaacgtgaat
      481 actctaaaac tgcgttgggt ttatcaactc cggcgaacag gaatcttcga atcttctccg
      541 atcgtcgtgg atggcgtgat gtacgtaacg gagcccccaa ctacagtgac ggccctcgac
      601 gtccgcaccg gtaggcccat ttggagatgg acggccgatc tccccaaaca cctgctgacc
      661 attggacttt ttcccacgaa ccgcggtgtc gctgttctgg gagatacggt ttacgtagca
      721 acgattgacg cgcatcttgt cgcgctggac gcgaaaacgg gcgccatgaa atggagcgtc
      781 gaaatcggag acaataagaa ggctgtggcc atcacgcagg cgccgcttgc catcaacgga
      841 aagatcatcg tcggcatggg tggcggggag ggcggattgc gcggatacat tgatgcctac
      901 gacgcaaaag acggcaagcg actttggcgc ctttacacga ttcccaccaa gggcgaaccc
      961 ggcgtggaaa cttgggaagg cgacagctac aaatatggcg gcgccaccac ctggaacact
     1021 ggcgcgtacg atcctgaatt aaatacgctt tattggggca caggtaatcc cgcgcccgat
     1081 tggaacgggg acatgcgcaa gggcgacaat ctgtattcat gctcgcttct cgcgatcgac
     1141 gcggacacgg gaaagatgaa atggtatttt cagttctcgc cccacgaaac gcatgattgg
     1201 gattcggcgg agccgccgat tctgttcgac gctgtgatca acggcaagaa gcgcaagatt
     1261 gtggcccagg cgaatcgcaa cgccttctac tatgtccttg atcgcgagac aggcaagttc
     1321 ctggcaggcg ccgcttatgc caaggaaact tgggcgaagg gactggacgc gaaagggcgg
     1381 ccgatcgaga tgccgaatat cgatccgacc acccagggaa acctcattta tcccagcatc
     1441 actggttcgg ttaactggac cagtccgtcg tacagtccgt tgactgggct cgtgtacgtg
     1501 gatacgcgcg agcaaggcgc ctactatttt aagggccatc cgacgatgga acctgcgaac
     1561 cctctggata tcggcggagg tggcggacag cgcgtagtct acggcgatga tgcgtacagc
     1621 gcgattaagg cgcttgaaag cacgacagga aaaataaagt ggcagttcaa gatggtcgga
     1681 gattcctgga ctggaacgct tgccactgcc ggaggactcg tattttgcgc ggatagccag
     1741 ggcaattttt ttgcgctcga tgcgaacgac ggcaaaccgc tatggaacat ccaactcgga
     1801 aactcagtcc gcgccaatcc catcacctac gccgttgatg gcaagcagta cgtggaagcg
     1861 gcggcgggta atgccgtatc ggttttcagc ttgccttagg catcgcggcc agatcgaaaa
     1921 atcgccgaag atccttctag tttgacgctt ggattgtttc taatttgcag cattttttcc
     1981 ttctaactag aaactcttag cggtcaaaaa acgtcgcggg aaggcgtcgc caagcctcta
     2041 gcgggcctgc ttcttgcggg agagtttaag cgttacgtcg atgtcgtgcc gggaatcgaa
     2101 actggaaact gtacggttcg gcgacgtcat gtccttgtgt tcggcatgaa cctcgtagtc
     2161 cacattgggg tccaggccgc tgaaacggta agcgccggcg tcatcggcaa tataggtctt
     2221 cacagtttgc gtcttcacat tcagcagata gactacgctt gatggaacag gattctcatt
     2281 cttatccaca acgctgccgt gaatagtgcg cagttcggct tcacccctct tatcctggtt
     2341 gcgcgcactt accggagaaa tagcgaaagc cgttagcgca agtaggagag caaggccgca
     2401 aaaggctcgg atggtcaaaa tctcctctga tcgacgcggc ttaaatcgac gagagtctgt
     2461 tctcctgaaa cgacggcgaa ccggcgagga gacgcgggca tttcattttg ctctgcggac
     2521 cataggtgca gagattggtt acagggagtc accgtcttct tcctcttcgc cttccggctc
     2581 ttcctcgtcg tcatcgtcct tcgtttcatc gtcttcctct tcttcctcct catcttcgga
     2641 gagaacgatg agatgtaccg ggtgggtctg cgtgacttcg agttcgacgt cacattccgg
     2701 acagcctacg atttcgcctt cctcgacttc gtcttcgtcc aagtcaattt cagcatcgca
     2761 ttcagggcat tttgtcacgg aactctttcc tcctgaggac gttcgctgga cagcgtatta
     2821 tataacgccg gtgtcaagct tttccccgag gttcgcgtaa aaggacatgc ctgagcgccg
     2881 cgaatatccc gagcgcccca taattggcgt cggcggggtc gtgatttcag acggccgcgt
     2941 tttacttgca cgacgcggtg gcctgccctt gcaaggccag tggtcgattc ccggaggaat
     3001 tcttgaagtt ggcgagacgc tgatcgaggg agtgcagcgg gagctcgcgg aggagacggg
     3061 aatcgaggtg cgagtgcgaa ctctgatcga ggtcttcgaa aggatcgatc gcgactcgtc
     3121 gggaaagctg caataccact tcgtcgttct ggactatttg tgcgaggcgg ttcgaggcac
     3181 cgcgcgggcg ggaagcgatg tcaccgaagt gtcctgggct gctcccgccg agctcgagag
     3241 atacgcgctt acggaaatcg ccggacgcgt gatccggaag gcgtttgagc tggcgggtga
     3301 gggtgattga cgtgaatgac gaaaaactag ctggcggcag cttcgggcag tcgaccaaag
     3361 cggcgctcgc gaccgagaaa atcttcgaca gcggcttcca gatcggctgc ctcaaattcc
     3421 ggccacattc gcggcgtaaa tagcagttcc gcgtaggcgc attcccacag cataaagtcg
     3481 ctcagacgcc gctcaccgcc ggtacgaatc agcaggtcaa cgtccggcgc cgaacccccg
     3541 ccatgggtta cctgcccgag ccggcgcgcg aactctcgat ccgagacttc cagactggac
     3601 atcatccagc aggacgcacg aagaatcgcc tctcgcgaag aataatcgag cgcaatacgc
     3661 agttccagtg cgcggccggc cacagtgaga tgctcagccg cctcgattgc atccacaaga
     3721 gtggcgggaa tgcgatcgcg ccggccgatc acccgcagcc ggattccctg cgctgcgcat
     3781 tcatgagctt ccgttcgaag atagtcttcg agcaagccga gcagggaagt tacttcacca
     3841 gaagggcgtt cccaattgtg cgaggagaag gcatagaggg tgagggtccc tatgccgagc
     3901 gatagggccg cacgagcgat gcggcgcacg gcagttacgc ccgcgcgatg gccttccgct
     3961 cgtggaagcc ctcgggaagc ggcccagcgg ccattgccgt ccagcaaaat ggcaacgtga
     4021 attttcggat cgggaataag cttggcggcg ctgttgccca tggcctcgaa ttaataatcc
     4081 tttctaaccc tcccgggtgg cgcgaataag ggcttccagg tgttccaagt atttctccag
     4141 cgctttacgg ccagcatgca tcagccggta ctcagtcttg ggcattcggc cggaaaatga
     4201 cttgttgcaa ccgatgtagc ccgcgtcttc caatttgcgc gcatggacgc tgaggtttcc
     4261 gtctgtaatt cgaagaaaat ccttcagctc cttgaacgtc agggacgcat tgaccgccag
     4321 agcgctgacg attgccaggc gggtacgctc gtgaatcaaa cggtcgagac tgacggccgg
     4381 tactttcgca ttcagagcac cttgcgggct gcgcttgggc ctctcttctt ttgggaggcg
     4441 caacattccg gatttagcca ccatatttcc tcgcgatcac cgcgccgaag atgatgtgaa
     4501 gtccgccgaa ccctgcagca agaaaagcat tgctccagct ggaaggagag aaaagggcca
     4561 cggctcccaa aaccatgaag caaagcccca tgagaggtac gatgcgtatc gaaagcatgc
     4621 cacccgtaac cacggccgtt ccgtagagca gaagccacac accggcaatg gagcctatca
     4681 ggcccgcgcg caccagcacc agcgtcagga gaagacccac aaagaggggc ggagcgaagc
     4741 tgtacacaaa tcgccgccct ggcccggaaa gaatcgccgt tcccgcgtcg cgcgccttcg
     4801 aacaggtggt ccacgtggca atggcaacag ctaccagcgc ctcgcaggcc caggtaatca
     4861 accatccggt tgaagactgc tgcagggacg cgatggcggt cgccccgatg gccgtaatcc
     4921 ccatggcgat gccgccccag cccggcacgg cggtgaacga ggaggcgcgc tccatcgttt
     4981 cgcggatgaa gcggatattg tcgcgagcgc gatcatccat cgccacaggc gtaccgccag
     5041 attgccgcga gtccaagggc atcatggacg caggctagca tctgcacgca tgctgtcaag
     5101 tactttgtgg cataaagtgg gttctagata aagggccagg cggcttgtcg cgtgcgaacg
     5161 gcgtaggcgc aaattactgg cggttcgctg gatagcggcc gaacacacgg ttccagcaga
     5221 tgtacatcag gtctccaaac atcaagacgc tgtcccaagc cacatgcact ttagtggccg
     5281 ggtcgtgggc ccagcgaacg ggcacctcgg ccgtgcgcaa gccgtgacgc tgtgcgagaa
     5341 agagcacttc cggatcgaaa ccgaacccct cgattcgctg ctgctcgaag ataatccggc
     5401 acggctcacg aaggaacgct ttaaaaccgc actgcgtgtc gtggagcgcc agcccggtaa
     5461 agagcctgac aagcacattg aaaataatcc cggcggtttc acgaaagacc gattgatggg
     5521 ctgcgatcaa cgaccggtca atggcgcgcg agccgattgc gatgtcgtga cccgcggata
     5581 ttgcggccag aagcttttcg gattcctcga cgggcgcgga caaatcggca tcggtgaaaa
     5641 gtgctatacg cccgcgtgct tcgagcatgc cccgccgtac gctatatccc ttgccgcgat
     5701 tcctctcatt caaaaaaagt cgaaatccgc catttggggc cgctctttcc cactcctgag
     5761 cgatacgcgc ggtcccatcg gtcgagccat cgtcgacgac gatgatttcc aaggcattcg
     5821 caaatcgtgg atcccgtctg aaatactcgt gaacgcgaga gagcgtgcgt tctaatcgcg
     5881 cctcttcgtt ataggccgga acaataatcg aaagttccgg ggtgcctgag ttcatccgcg
     5941 gttggtttcc ctggcatctt ggcgctggcc gtaacgtcca actagcggaa cgaatcggca
     6001 ggcatagagg gactgccggt caatgcgtcc ttcctttttc accaagcgaa gcaattcctg
     6061 atgctcatct tgtcctatcg gaatcaagag ccttcctccc tcagccaact cgtccactaa
     6121 cggctgtggc accgagggtg ccgcggcggt gacgagaatc gctgcgtacg gtgcgcacga
     6181 tggccagcct gcggagccgt cgcctatctt caggctcacg ttgcgatacc ctaatcggtc
     6241 gatccggtcc gccgcggccg cggctaacgc cgggcgagcc tcgattgcga tgacctcaag
     6301 agcaagttgg gaaagaaccg ctgcttgata acccgatccg ccgccgattt ccaacacgcg
     6361 ctcatgcccc tggagcaaca gagcgtccgc catggctgcc accataaagg gttgggaaat
     6421 cgtttgttct tcgccgatgg gcaatggctc atcggagtac gcacgggact tgtattcggg
     6481 gggcacgaac aaatgtcgcg gcaccgctcg catggcgttc agaacgcgct cgtcgcctat
     6541 gcccctatcg cgaatctggc gggcaaccat ctcgtggcga gcagcttcga actccgcggt
     6601 gtcgccttga acgaacttgc cttcgccgtt cacggattct cttcgatttc aaaaaagtcg
     6661 gcccggcggg gcctctcttc ggtactgata tcctaggctg caacgccctt tggtctttgc
     6721 cgaagctatc acatgcgagt gaggacctgc cactgaaacg tctgctccca aattgcatat
     6781 tcaattgtcg ttggatacga tgcgtgaaag agttcgccgc ggtgggaagc acgcccggac
     6841 ataaccagga gatactcaaa tgaaaactcg aaccgctctg atcgcagtcg ccttttgtat
     6901 tgcagtaccg gtcggagcac agacacctat gccgggaact caacaaaaac cggcgcagca
     6961 tcagccaggc actccggccc cacataagcc cggccagccg gcgaccgatc aaaccgaaaa
     7021 agccgaaaaa cccgagccag gaaccgcgac ggccaagccc gatgctgcca aagaggccgc
     7081 gattcgccac ctcatggacc ttacgcagag ctcgaaactc ggcgataaca ttcaaaccta
     7141 cattacgaat caggtccgca ccggcctcgc gcgcgcgcta aagccggacg cgttgccgaa
     7201 attcatggac gaattcagcc aaaaattcgt ctcagccgcc cccgcatcca gcgtaacgga
     7261 tgcaatggtg ccaatctatg ccggcgcttt ctcaacagaa gatattcagg gcctgatcca
     7321 attctacgag tcgccgctcg gacagcgcgt agtgaaaaat ctccctaccg tggatcggca
     7381 gtcccaggag gcaggcgtgc gaatcgaaca ggccgcggcg ctagccatct taagagacat
     7441 gtcggaggag taccctgaac tcaagcaaat tcttccgcct gggaccgagc agccaacgcc
     7501 cggttcggag acacctccag cgccgccgca gcgatgatga ggtcgttagg ttctatcgga
     7561 tttgtcagcc gggaaatatg actgaaaaag a
//
LOCUS       DATNDT010000375         5047 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3535698, whole genome shotgun sequence.
ACCESSION   DATNDT010000375 DATNDT010000000
VERSION     DATNDT010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3535698"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..572
                     /locus_tag="VN788_12060"
     CDS             324..572
                     /locus_tag="VN788_12060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21307.1"
                     /translation="MSLLKAPSKQPKTTTLQVRLEEEVRRNLDRYAEFIGANASYVVS
                     EALKLLFKRDDEFKRWLTDHPTKIEEQTRKGEVLTKTA"
     gene            1042..2223
                     /locus_tag="VN788_12065"
     CDS             1042..2223
                     /locus_tag="VN788_12065"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HXU21308.1"
                     /translation="MLFERKRYQFGYVRQKPRKTGPHVWVWEYRDRSGSHSVILGTID
                     QMSETDAWKATEGRRLILNDPHVADQVSFGAVLDRYMLEALPERKSTRCTYLSWIRSR
                     IRPKWGDCPIVHVKPLAVELWLKTLPLSGRSRGHVREVMHMLFDWAMRWELIPHDRNP
                     MTLVRVKGCSKRTRVPKILTIEEFNRLLEKLKEPYRTMALVALFLGPRVSEIAALKWS
                     DIDWDKATISIARSWVVGEVGDTKTEGSAKPLPMDSDLAIVLKEHRERSKNYQSQWIF
                     PNPATGNPFWPSKIRENHLVPAAIAAGIGPVGWHTFRHTYSSLLREHEVDIKVQQALL
                     RHADIRTTMNIYTQAVPRAVREANRKVVRNILGKQGGIEAAIRVPETAKSFQISPSPS
                     A"
     gene            2531..3325
                     /locus_tag="VN788_12070"
     CDS             2531..3325
                     /locus_tag="VN788_12070"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HXU21309.1"
                     /translation="MTEQTIVVTGSKGGVGTTTVALNLAVQLAMQTGRRVGLLDLARP
                     FGQISLMLDLEPRFTILDALERIVRLDEALLASMAMRHKTGIEILAGPLHAPMKPEQR
                     QSVTLAALTKLVEIAAAAFEFTIVDMGVVNAAEWSPVLTHAGTLLLVAEPSVLALGMV
                     GRHISAAAAVGINCERIQVIVNRWRQNDDAMLSAFEKQSSRPVLTRLPNDYRQLTEAL
                     TLGMPLTGSANNVLLSRYRALASWIAANCSAQVREDAAQAAFEPVE"
     gene            complement(3434..3976)
                     /locus_tag="VN788_12075"
     CDS             complement(3434..3976)
                     /locus_tag="VN788_12075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21310.1"
                     /translation="MNKVLRNLAFALTLLVVPVGIGAQGGQGGGGFGGRGGGGGRGQG
                     FPGGGRGARGGFVPNGPAGALAGRTDIYNSTNGVEPVGKATAAGEHFFIIGSVDQSQQ
                     QMLLKWPTEVTLLLKVTPDTKFSSEAGKPLKFADFRAGDTVWVSYKGTGADATAVHVR
                     AGVMTVAELHQYYLDYPVIK"
     gene            complement(3969..>5047)
                     /locus_tag="VN788_12080"
     CDS             complement(3969..>5047)
                     /locus_tag="VN788_12080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU21311.1"
                     /translation="VQDQIKSLGDNMIMVMAGSVNQGGVRLGGGATKTLTAEDGEAIR
                     TQVPNVIAESPSVGANAQMVNGNQNWSSRIQGVSPDFFTIRSWPVSEGSEFTQRDVAL
                     AANVCVIGQTVADQLFTGGADPVGSLIRINNLPFTVVGVLVKKGQSSFGQDQDDTVIV
                     PYTTVQKKITGISWVQQIQISVATENDIEGAISSMSSLLRQRHKLRPTEDDDFIVRSP
                     DELAQASAQTSQILTYLLGSVASVSLLVGGIGIMNIMLVSVTERTREIGVRMAVGATE
                     QDVKMQFLSEAMVLSFLGGSVGIVLGVIASAIISNVLQWTTLVSTTSIIVAVFFSAGV
                     GVFFGYYPASKAAQLDPIEALRYE"
BASE COUNT         1196 a         1427 c         1381 g         1043 t
ORIGIN      
        1 agcaagagat ggggtgacgg ctggcaagtg cgcggcactt caggtacggc ttcagttccc
       61 aaatcaagac cagctcaatc acagattctt gaaagaagag cggatacata aatgcaacgg
      121 gtgacatctg cacgcggaca aaacacaaga aagggcggga gcgaccccgt tgtgtgcggc
      181 ttcgagccgc ccacacgttc gtggggtcgc tcccgcccgc accgaattgc tcggtgccgt
      241 gttttgtccg cgtgcagaga tcggagcaaa cagcttgtaa tgcaagccgt actgcacagc
      301 gaaagaagaa aggagcaaaa cagttgtcac tactgaaggc accatctaaa caaccaaaga
      361 ctacaaccct ccaagttcgt ctagaggaag aagtcaggcg caacctggac cgatacgccg
      421 aattcatcgg cgcaaatgcg tcctacgtcg tcagcgaagc actcaaactg ctcttcaaac
      481 gggacgacga gttcaaacgt tggctgaccg accatccgac caagatcgaa gagcaaacac
      541 gcaaaggaga ggttcttaca aagaccgcgt aacagacatg aagcctcgtt caacacaatc
      601 cggcacgcgc gagaatacag cgtcctcaag actgcatgcc ccgcgccgta gtacgagcat
      661 tccccctgtg gttcacgatc tgttgcctgc aatcgatcaa gaagacgcaa gcgtaaagac
      721 atcgcaaccg ccgcttcgac agccaatcga attgcaactc ccggaaccca tccaggggac
      781 cacaggcgga agagacggta cattgacact cgtcggaggc acgaatcggc cgggtctcga
      841 acgccctttg acctgcgggg aagccgccgt atttgtgcga gtacatccca agacgatcaa
      901 acgaatggcg cgcaacggcg agatgcccgg tcactttcgc tttggccgct ggtttttcta
      961 cgcgtcagag ctggactgct ggatgagaac agagttacat tccgtacgcc acccttgatg
     1021 ctcaaacaca aggaggattg aatgttgttt gagcgcaaga gataccagtt cggatatgtg
     1081 cgccagaaac cgcgcaaaac tggcccgcat gtctgggtct gggagtatcg agatcgcagt
     1141 ggttcgcatt cggtcattct gggaacgatt gaccaaatga gtgaaaccga tgcttggaag
     1201 gctacagagg gccggagatt gatattgaac gaccctcatg ttgccgacca agtttcgttt
     1261 ggtgcggtgc tggaccgtta catgttggag gcactgcccg agcgaaaaag cacccggtgc
     1321 acttacttaa gttggatcag gagtcgcatt cggcccaaat ggggagattg cccaattgtc
     1381 cacgtgaagc ctctcgctgt cgaattgtgg ctcaaaacct tgccactttc cggcaggagt
     1441 agagggcacg ttcgcgaggt gatgcacatg cttttcgatt gggcgatgcg atgggaactg
     1501 atcccgcatg atcggaatcc catgactctg gttcgcgtaa agggatgttc taagcgaacc
     1561 cgagttccga agattctgac cattgaggaa ttcaatcggc tcctggaaaa gttgaaagag
     1621 ccttacagaa cgatggcact ggttgctttg ttcttaggtc cacgagtcag tgagatcgct
     1681 gcactgaaat ggagcgacat cgattgggac aaagcaacca tctctattgc aagatcatgg
     1741 gtagtcggtg aagtcgggga taccaagacg gaaggttccg cgaaaccctt gcccatggac
     1801 tcggatttag caatcgttct gaaggagcat cgagaaagaa gcaagaacta ccaatcgcaa
     1861 tggatctttc ccaatccagc gacgggaaat ccgttctggc cgagtaagat ccgtgagaat
     1921 catctagtgc ccgcggcaat tgccgcgggc atcgggcctg tcggatggca tacgtttcgg
     1981 cacacgtact cctcgttatt gcgggagcac gaggtggaca ttaaggttca gcaggcgctt
     2041 ttgcgtcatg cggacatccg aacaacgatg aacatctaca cgcaggctgt accaagagca
     2101 gtacgcgaag caaatcgcaa agttgtgaga aatattttag ggaaacaagg cggcattgag
     2161 gcggcaataa gggttccgga gactgccaag tctttccaaa tcagtccgag cccttccgcc
     2221 taagtccttg aaaactcgag cgggattaat ttcgattccc atcgcccgct ccataaatcc
     2281 ggttgctatc gttatagacc ccgaccttct cacagagttc aatcttcccc tgctaccgag
     2341 agccatattc cgacacactt gtcgcgcgag gtatgtctga agtctgtcta aagttagaat
     2401 tccctaatcg tccgcgcgcg atttggtcct ccggctggca ttcgggatgc actactcgaa
     2461 atacggaagt gcgtagtctg cgcggcatgc atagccgtag caggcgctac acggaacgac
     2521 ttcgagcggc atgactgaac aaacgattgt cgtcacaggg tcgaagggcg gcgtggggac
     2581 caccacggtg gcgctgaacc ttgccgtaca gttggcgatg cagacaggga ggcgcgtagg
     2641 tctgctcgat ttggcgcggc cgttcggcca gatttcgtta atgttggatt tggagccgcg
     2701 cttcaccatt ctcgatgcac tcgagcgaat cgttcgcctc gatgaggcgc ttctcgcgag
     2761 catggcgatg cggcacaaga ccggcattga gattctcgcc ggtccgcttc acgcacccat
     2821 gaaacccgag cagaggcaga gcgttacgct cgctgcactg accaagttgg tggagattgc
     2881 agcagcagct ttcgaattca caattgtgga tatgggagtg gtgaacgcgg cggaatggtc
     2941 gccggtgctt actcacgccg gcactctcct gcttgtcgct gagccgagtg ttcttgcgct
     3001 cgggatggtc gggcgtcata tatcggctgc ggccgccgtt ggcatcaact gtgagcgaat
     3061 tcaagtgatc gtaaatcgct ggaggcaaaa tgatgatgca atgctctcgg cgtttgaaaa
     3121 acaaagctcg cgtcccgttc ttacacgcct cccgaacgac tatcggcaat tgaccgaggc
     3181 cttgaccctc ggcatgcctc ttacgggaag cgccaacaat gtgctcttgt cgcgttatcg
     3241 cgctctggca tcatggatcg ccgcgaactg ttccgctcag gttcgggaag atgctgcgca
     3301 ggctgccttt gagcctgtgg agtgactggt gcgtctggcc tcagcattta gcagtgaaaa
     3361 atcggcccgc agaaaactaa aatcccgccg agcgcatcat gcccggcggg atcgtcttat
     3421 ttgaggaatc cacttacttg atgaccggat aatccaaata gtattggtgc aactccgcca
     3481 ccgtcatgac gcccgcgcga acatgcacgg ctgtcgcgtc ggcacctgtg cccttgtacg
     3541 aaacccaaac cgtgtcgcct gcgcggaaat cggcaaactt cagcggtttt cccgcctcgc
     3601 tggagaactt tgtgtcaggc gtaaccttaa gaagcagagt cacctctgta ggccatttca
     3661 gcagcatttg ctgctgcgac tgatccactg acccgatgat aaagaaatgc tcgcccgccg
     3721 cggtcgcctt tccaactggt tcaacgccat ttgtggagtt atagatgtct gtccgtccgg
     3781 ctagcgcgcc agccggtccg ttggggacga atccgccgcg tgcaccgcgc ccaccgcccg
     3841 ggaagccttg tccacggcca ccaccgccac cgcgtccgcc gaaacctcct ccgccctggc
     3901 cgccctgcgc accgatgccg accggcacaa ccaacaaagt caaagcaaaa gccaaattgc
     3961 gaagaacctt attcatatcg aagcgcctca ataggatcaa gctgtgccgc cttgctcgcg
     4021 ggatagtacc cgaagaaaac gcccactccc gccgagaaaa agacggcgac gatgatcgaa
     4081 gttgtggaaa ccaaagtcgt ccactgcaaa acgttggaaa tgatcgcgga cgcgatcaca
     4141 cctaagacaa ttcctacgga tccgcccagg aagctcaaca ccatggcctc gctcaggaac
     4201 tgcatcttga cgtcctgctc cgtcgcaccg acggccatgc ggacgccgat ctcgcgcgtt
     4261 ctctctgtta ccgagaccag cataatgttc ataatgccga tgccgcccac aagcagagaa
     4321 accgacgcta cgctgccaag cagataggtc aaaatctgcg acgtctgggc agaggcctgc
     4381 gctagttcat ccgggctgcg gacaataaaa tcgtcatcct ccgtcggacg cagcttgtgc
     4441 cgctggcgaa gcagcgagga catcgacgag atagcaccct cgatatcgtt ttcggtagcc
     4501 actgagattt ggatctgctg cacccaggag atgccagtga ttttcttctg gaccgtggtg
     4561 tacggcacga tcaccgtgtc gtcctgatct tggccgaaac tcgactgtcc ttttttaacc
     4621 agtacgccca cgactgtgaa cggcaaattg ttgatgcgga tcagcgaacc aaccggatcg
     4681 gccccgccgg taaaaagctg gtccgccact gtctgtccga tgacgcaaac gttggccgcc
     4741 aacgccacat cccgctgagt gaattcactg ccttcggaaa ccggccacga acgaatcgtg
     4801 aagaaatccg gtgaaacgcc ctgaattctc gagctccagt tttggttgcc gttcaccatc
     4861 tgcgcattgg ccccgaccga tgggctttcg gcgatcacgt ttggaacctg ggtcctgatg
     4921 gcctcgccgt cttcggcggt gagtgtcttc gttgcaccgc cgccgagacg cacaccacct
     4981 tggttaacgc tgccggccat gaccatgatc atgttgtcgc ccaacgactt gatctgatcc
     5041 tgaacct
//
LOCUS       DATNDT010000376        39059 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3256884, whole genome shotgun sequence.
ACCESSION   DATNDT010000376 DATNDT010000000
VERSION     DATNDT010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 39059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39059
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3256884"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..923)
                     /locus_tag="VN788_12085"
     CDS             complement(84..923)
                     /locus_tag="VN788_12085"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HXU21312.1"
                     /translation="MVAFHLGAIAALLFFTWKALALAAVLWWVAGSLGIGMGYHRLLT
                     HRGYKTPKWLEYFLTVCGTLALEGGPIGWVATHRVHHQNTDREGDPHSPQDGGLWAHI
                     GWIMTGKAIQNPSRELLCFVPDLRKDKFHVWINKWHWIPLVVIGLGIFAIGGWPFLLW
                     GIFFRTVIGLHSTWLVNSATHMWGSQRFPTGDMSTNSFWVAMLTFGEGWHNNHHAAPQ
                     SARHGLAWYEFDLNWYGIVALQRLGLAWKIKLPQLKPAREARVVIPARADKANAAAID
                     TLQ"
     gene            complement(1462..2040)
                     /locus_tag="VN788_12090"
     CDS             complement(1462..2040)
                     /locus_tag="VN788_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21313.1"
                     /translation="MCKVYKQFILLLGMFLCANVATAAQMIALQIVPAQEAITPISAV
                     LRTVPMPVPAVRFRSSTNRGKSVFHFSYSLARPYARDHSLQDLESLSSMREVKTLFST
                     RSSLPLVELWGGRLRLDGFTSTLDMQNVQLGPSGGGSLQDFRLRRQNYPDEPRSVTLD
                     GVSLTFHFGRSARIGRPVQIWRCLARIVSALR"
     gene            2300..3154
                     /locus_tag="VN788_12095"
     CDS             2300..3154
                     /locus_tag="VN788_12095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21314.1"
                     /translation="MRILTKSPVLFLVALTASSALFGQTGGSSPAKSQSHASALDAHQ
                     IVERSVAATERNWHARDDYTYIERDEDQRLDSLGNVKSENVDVTKTIVVNGAPFAQLV
                     EHNGRPPSAEEQSRSSEKLYNLEHETPQERIIRLGKDEESKSFLRNVVEAFDFQLVGE
                     GLAAGRPAYVLRVTPHPGYQPHGKYGKIFSKVEGKLWIDKQNFGWIKVDGRVTRAFSM
                     GLFVARMQRGSHVTLEETCMGDGVWLPKRIELRASARILFFKSLDIDRILTYSDYLLA
                     TVPYSASK"
     gene            3741..4763
                     /locus_tag="VN788_12100"
     CDS             3741..4763
                     /locus_tag="VN788_12100"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HXU21315.1"
                     /translation="MAQLSAHGAFDCIAREHVAQLPLAVRQALNEKKLREELECARKS
                     LRHSQSLYRALVDNPAYGIYRCNAERKIVEVNQALLTMLGYSTKEELLAENELTALLS
                     QLGTSSPWAGRSRETSQIEPVETEWKRRNGTMLKARISGRAVFDDHGNFAGHEIIAVD
                     ITEQRTLEDQLRHQASTDSLTGLANHRRLVEALHAEICRSKRTAREFSLALLDLDELK
                     QINDRFSHLAGDRALCRLGQILTDCCRSIDTAARHGGDEFALVLPETGAAAASLLARR
                     ICELLEREAEEPALSVSVGIASYPKDADTIGTLLYAADRALYAMKRKQPRAAQSGLGS
                     IHNVAT"
     gene            4778..5290
                     /locus_tag="VN788_12105"
     CDS             4778..5290
                     /locus_tag="VN788_12105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21316.1"
                     /translation="MDEYRFRRRDWSKEPEPPPNSEQGIQLALSVEKQLLHSISAYAP
                     LPEVLNKICSALDYQIGNVVSVISLPWDNASKRGGIAVSAAVFGLHIFCTESVADENG
                     ELLGFLEMYSCVPRSPSPGECQWIKWAGCLAGVAIKRSMETGDQRDRAVPQNQPVRGR
                     LLKRLVFIRG"
     gene            5451..5603
                     /locus_tag="VN788_12110"
     CDS             5451..5603
                     /locus_tag="VN788_12110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006983692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lmo0937 family membrane protein"
                     /protein_id="HXU21317.1"
                     /translation="MLAVIALVLIVLWLFGFFAFHITTAFIHVALIVGLILLVLHFMR
                     GRRARV"
     gene            complement(5650..6333)
                     /locus_tag="VN788_12115"
     CDS             complement(5650..6333)
                     /locus_tag="VN788_12115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU21318.1"
                     /translation="MPKTGARKTKLIPKRNSSFDLRVFLDTAGVARRVKEFKGGEVVY
                     SQGDAAHSVMYLQEGGVKLTVISEAGKEAVVAILGPGDFFGEGCLAGQSVRMGTATAI
                     TPTTLLVIEKSEMLKVLHEQSALSDRFIAFMLARNIRIEEDLVDQLFNSSEKRLARTL
                     LLLARYGKEDQPHGVLPKISQEMLAEMIGTTRSRVNFFMNKFKKLGFIEYNGGLQINT
                     SLLSVVLHE"
     gene            6682..6966
                     /locus_tag="VN788_12120"
     CDS             6682..6966
                     /locus_tag="VN788_12120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21319.1"
                     /translation="MERTQSKTPPRHFRTMVQADVPQGRNGKHKKIVTTILNDLDGLK
                     ANSALKVPLAELAESKERVRSALSRATRKAGRKVATASDATFLYVWNVIE"
     gene            complement(7846..9033)
                     /locus_tag="VN788_12125"
     CDS             complement(7846..9033)
                     /locus_tag="VN788_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXU21320.1"
                     /translation="MAQALAHQLATSETNVLRMLSQGAPMMEVLNELCNLIHAKSPSV
                     IPTVLLPDPDGTHLRLAAGPKVTKIWNDAFEGLKVPSNSFHGAAGHSEESVPLADMRS
                     DPSFAACWDLALRQGIQAAWSAPIFSKDKEILGALILFYPTSQRPGERDLDLMEQVIH
                     MAAIAIESHHKEEELREFSRRLYQSQDEERRRIARDLHDSTGQKLSLLAIDLSMITDE
                     LPAESRNKILLQCQFLIGSISDEIRTLSYLLHPPMLDECGLNTAIQWYVSGINQRHGL
                     HVKVEISHELRRLSEEAELAIFRVVQASLTNVHLHSKASEAKVKIEQNLDGVTITVSD
                     DGQGIPDGVLDHSSRIKTVGIGITGMRERVKQLGGSLEIETGHNGTSVRATVPSHHFR
                     TAE"
     gene            9336..9743
                     /locus_tag="VN788_12130"
     CDS             9336..9743
                     /locus_tag="VN788_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21321.1"
                     /translation="MKSLFVVVSLLTVLWWPWSSGKKYRMTADASVPAASGTVQAQKD
                     KANGNTNMDIKVNNLADPTRLTPSENVYIVWVRPNGGAPVKEGAIGVNNGLRGELNVV
                     TTSKDFDVFVTAEQSESVILPSGVEVLRAHVSS"
     gene            10104..10295
                     /locus_tag="VN788_12135"
     CDS             10104..10295
                     /locus_tag="VN788_12135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HXU21322.1"
                     /translation="MPLIQIIVALIVVGVLLWLINRFVPMQRTIKSILNAVVVIVVVL
                     WLLDVFGLFHSLARIHVGT"
     gene            10412..11143
                     /locus_tag="VN788_12140"
     CDS             10412..11143
                     /locus_tag="VN788_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21323.1"
                     /translation="MKRIGAISTAALFLLLGIAAPAYAHQDQQEKPPKQEQRAKPSKH
                     QQQAKAAAGQQQSKRQQQQTKANDQQQQQQAKRRHQQTKADDQQQQQAKREQGQRQQR
                     AGQPSRIQKVNMQPLQVQRQQEQHQQRAWQEHRANNWRSEHRTWQQRGGYNGYRIPDD
                     RYNRYFGREHAFRIYSYPVVVVGGYPRFQYNGFWFSLADPWPEYWSNNWYENDDVYIE
                     YSVDGYYMYDTRYPRDRIAVSVYVN"
     gene            11271..11426
                     /locus_tag="VN788_12145"
     CDS             11271..11426
                     /locus_tag="VN788_12145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018443988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HXU21324.1"
                     /translation="MLGTILIVVLVLALFGAWPRWSHSRNWGYGPTGGLGLVLVIVVI
                     LLLLGRI"
     gene            11635..12972
                     /locus_tag="VN788_12150"
     CDS             11635..12972
                     /locus_tag="VN788_12150"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HXU21325.1"
                     /translation="MNSERRIRPRASSSGALSGWCPWGIRIVTFLLLAIMCSSASFAY
                     SVLTHEEIVDLLWTSEIRPLLLERFPTLTEEQLKEAHSYAYGGAVIQDLGYYPFGSVE
                     FSDLVHYVRSGDFVRELLSQSRDANEYAFAMGALAHYASDIAGHPAVNRAVSIEYPKL
                     RAKYGKSVEYAENHSAHLKTEFGFDMEQVAKNRYASQQYHDFIGFQVSKPLLERVFPV
                     VYGVRLEEVLPREDLTIGSYRWAVSRMIPEMTKIALRTHKKDMMRETPNFAKRKFLYR
                     LSRSDYERQWGKDFKKDGFKTRLFSMLLRYMPKIGPFKSLAFNNPTAQTENLYFKSIN
                     ASVDQYRAFLEQVRSGSLELDNRDLDTGKETGPAEYTLTDETYAKLLGQLADQKFDLT
                     SVDLRDNILHFYSDLSCPIETKNDTVRWRSLLSSLDQLKLVAPSRIVGVKRAN"
     gene            13162..13875
                     /locus_tag="VN788_12155"
     CDS             13162..13875
                     /locus_tag="VN788_12155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HXU21326.1"
                     /translation="MKRYLISAACFALAAGVVYAAQLNKEQKRLESCGTVMQEIMGTP
                     ENIPQEVLEKTDCVIVFPSVLKAAFGIGGSYGRGAMVCRAGKNFKGPWGAPAMYALEG
                     GSIGFQIGGQATDLILLVMNERGASSILNSKVKLGADASVAAGPVGRDASADTDAYLR
                     SEVLSYSRSRGLFAGISLEGSTLRPDGDATADVYGQKLTAREIVLGGKIGIPASGRHL
                     VAVLQKNAPHNESKESAQQ"
     gene            14086..14262
                     /locus_tag="VN788_12160"
     CDS             14086..14262
                     /locus_tag="VN788_12160"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXU21327.1"
                     /translation="MFDRDQALALQEQIRVRAYEIYSGRGRADGKELNDWLTAERELT
                     EQSSVVMRKTIIAS"
     gene            complement(14597..15568)
                     /locus_tag="VN788_12165"
     CDS             complement(14597..15568)
                     /locus_tag="VN788_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HXU21328.1"
                     /translation="MTQYETLFLRHPANPILTRKDWPYSMNSVFNPGATLLRDGTTLL
                     LCRVEDRRGLSHLCAARSLNGVDGWQIDPEPTLLPDAKQYPEEVWGIEDPRITYVPEL
                     KKFAVAYTSYSRGGPGVSLALTEDFRSFERFGVIMPPDDKDAALLPCRIGGFWALVHR
                     PMTPLGAHIWISYSPDLHHWGNHKLMLEARRGAWWDANKIGISPPPIETSRGWLMIYH
                     GVRRTPSSSIYRLGLALFDLEQPDKCLLRGDSWMFAPEAEYERQGDVQDVVFPCGYTV
                     ARDGDTVNLYYGAADSSVALAHGSIRSLLTWLDAKGHPEHSGDRRHR"
     gene            complement(15642..18035)
                     /locus_tag="VN788_12170"
     CDS             complement(15642..18035)
                     /locus_tag="VN788_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010570123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU21329.1"
                     /translation="MSGPKIAMSRTESLAETAMLIEMQSPVLEEKLVTLTPAPARLAL
                     PTRIAVVGNYLPRCCGIATFTTDLCDAIHAEYSNTELLALPVNDTEEGYNYPARVRFE
                     LAEDNVASYHQAADFLNFSNADVVCLQHEYGIFGGSAGGHILELLRRLQMPFLTTLHT
                     VLRQPDPDQYAVMMEIVTLSDRLIVMSQHSAEILQEVFHVPSSKIDMIPHGVPDLPFT
                     DPNFYKDKFGTEGKDVLLTFGLLSPNKGIENVIRALPEILCRHSNVVYMVSGVTHPHI
                     LRREGDKYRHSLQSLAKELGAEDNVIFRNRFETPQGLVELIGAADIYITPYKHKAQVV
                     SGTLAYALSAGKAIISTPYLHAIELLSDERGALVPFDDPGAIAAKTIALLDDEMGRHA
                     MRKRAYLYARDMVWKRVAQKYMKSFDDVYNERLRTPRATFSAQNTPKVLDRLPAIKLD
                     HLLRMTDQTGIVEHAVFVVPNYPEGYTSDDNARALIVTTLLEGAAARMPTGLLDLGSR
                     YLAFLWLAFDPDTKRFRNSLSYERQWQEPEGSEDSHGRALWALGSVLGRSKNDGLRGA
                     AGRLFEIAVPAAVEFKSPRACAFALLGLQEYLDSFPGDRAALAAAEALAKQLLNSYRA
                     NHSAEWNWFEDVLAYSNARLPQALIRAGMRTANEEMLSAGLGALNWLVTIQRCEEKGH
                     FVPIGSQGFYSKNSEKARFDQQPVEACAGVSACLEAYRTTGSGLWRTEAWRAFNWFLG
                     DNDLQIALYDSATGGCRDGLHPDRANENQGAESTLSFLMALLEMHQMEEAGGIRKKV"
     gene            complement(18074..18745)
                     /locus_tag="VN788_12175"
     CDS             complement(18074..18745)
                     /locus_tag="VN788_12175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU21330.1"
                     /translation="MKRKKFKNNRSVGAFDPQAFLDTAGAARKVIDYRRGETIYSQGD
                     VAETVMYIQKGGVKFTVVNGAGREAVVAMFGPTDFFGEGCMAGQPLRMGTTSAITPTT
                     VLAIQKKELLRVLHSEHELSDHFISYILARNIRVEGDLIDQLFNSSEKRLARTLLLLA
                     RYGKQEQPDRILAKVSQETLAGMIGTTRSRVNFFMNKFRKLGFIEYNGKIKINRSLLT
                     VVLHD"
     gene            19043..19234
                     /locus_tag="VN788_12180"
     CDS             19043..19234
                     /locus_tag="VN788_12180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21331.1"
                     /translation="MRNVPPDEYNGAVVYLQYHGHRITPVESFASSDPATPGTRVGRK
                     TLTAEEVVSLAVNKGWVLP"
     gene            complement(19485..20165)
                     /locus_tag="VN788_12185"
     CDS             complement(19485..20165)
                     /locus_tag="VN788_12185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21332.1"
                     /translation="MERIVTQKLLDVVKKVAKEELESEKWLRVVARLSGRSYEQMRVL
                     ILEFLDTGVFHLSMENCVSFLEPLIESWDIDPVTFAVEVVLNRRLAPGRPKRFEFVEK
                     ESSGEEAGFKEFLRNSSLSGAATEEEIEFLKRLIFSGKHPTALYYYRELQNLRDPLSF
                     QPLTSRTARTITRGRSQPEGSIAPMHKHREAYAFEKQLQLDGKKRATRRWSGGNNRQR
                     KKEKTSPS"
     gene            complement(20409..20522)
                     /locus_tag="VN788_12190"
                     /pseudo
     CDS             complement(20409..20522)
                     /locus_tag="VN788_12190"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            20702..21031
                     /locus_tag="VN788_12195"
     CDS             20702..21031
                     /locus_tag="VN788_12195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21333.1"
                     /translation="MYARKVSVQLKANSVAEFTLRMEKDVLPLLRKQKGFQDEITCVA
                     PGHKEAFGLSLWENKESADAYSHEPYGEVTKFLTGLIEGTAHVESYEVSNSTFHNVAT
                     AAATAKN"
     gene            21132..21395
                     /locus_tag="VN788_12200"
     CDS             21132..21395
                     /locus_tag="VN788_12200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21334.1"
                     /translation="MSLLLWVMEGVIAGWLMGKVMGSEGRDRVMNVIMGIGGAVAGAL
                     IIAAIHIVHGKMIYTNLAAISGGMLLAFLSRYVGGSREYGRSN"
     gene            21675..22346
                     /locus_tag="VN788_12205"
     CDS             21675..22346
                     /locus_tag="VN788_12205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21335.1"
                     /translation="MNIRRNVLICIAASLLCVPLGYGQDLSKYRNFSLGTSLAELSKQ
                     LKGKPADAQITYQNPALIQELIWLPGQSYPSPAQPESVQEIRFSFYNGELYKIAVTYG
                     NLATEGLTVEDMVTAISAKYGAATRPVAVPAPTALADTFTKQPVAFWEDAQYSLTLSR
                     SSLSDAFQLVMYSKQLNGQADAAIAEAVKQEREGAPQREIARAKKQIDDLETTRQANL
                     KVFRP"
     gene            22415..22729
                     /locus_tag="VN788_12210"
     CDS             22415..22729
                     /locus_tag="VN788_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXU21336.1"
                     /translation="MNITPLNDRVLVRRLEEKETATGGIVIPDTAKEKPQEGEVIAVG
                     AGKMEKGRRIPIAVKVGDRILFGKYTGSDIKVDDQEYMILREDEILAKLNGMVKAASG
                     KK"
     gene            complement(23005..23823)
                     /locus_tag="VN788_12215"
     CDS             complement(23005..23823)
                     /locus_tag="VN788_12215"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HXU21337.1"
                     /translation="MASELQRVGRSEWWLWFWTFAATVLSTTGLFLSFFRRLFLHKEH
                     FYEITSDQARWGLLSLLLLFNGWLLYRQWLFRRARKQADAQSERADAAEANQDPAQMD
                     ALTGLYTRAAADQRLGKEVARARRRNLPLSIVALHLDDLGEITQRFGKDASDKLVAEF
                     ANRLRKASRGCDFCVRLGKDDFLVILPECSLGGAKRVSDRVGSVSIKAAGEEMSVNYS
                     VGWIDYKPGEVPSDLFKRAGDVLRLYKDASNDTSFETATAQKSNLKMPRVPAHR"
     gene            complement(23999..24691)
                     /locus_tag="VN788_12220"
     CDS             complement(23999..24691)
                     /locus_tag="VN788_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21338.1"
                     /translation="MDHRSGKPMNHRNDKSRIFLLSPANLSGKRGKLMWGGFSRCAMA
                     LKLRDGGAPLGEVFTFVSGLYFRGKLAYARAFARPPRNAVGVMVITASHGLMTPDTIV
                     GIEELQAMAASSIDEREATYREPLERDARRLAARIGRCEVVLLGSVATQKYAEPLLGI
                     FGAKLMFPTEFAGRGDMSRGGLMLRAVRDGVPLTYVCLANSMRHGPRPPKLPRLSKSP
                     VSKEAVVGSVTG"
     gene            24867..25868
                     /gene="ligD"
                     /locus_tag="VN788_12225"
     CDS             24867..25868
                     /gene="ligD"
                     /locus_tag="VN788_12225"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HXU21339.1"
                     /translation="MAVRAKSGPTRAHFTRPAKTKRSATWAGFEIPASANEADLRVPG
                     GVVHLTNLRKVFWPERNLTKRDLLQYYLDVSPWLLPHLRDRAMVMKRYPNGIAGDFFF
                     MKRAPSPRPAYIPLCSIEHRSGNIIDFPVVEHLAALLWVVNLGCIDLNPWYARCDDVN
                     RPDFLHFDLDPVPGATFDRVLETALLVRDSLAALKIKSYAKTTGSRGIHVYIPIMRGP
                     LQKQVWSFAKSFSVELANRHPKLITSEYRVAKRPAGRVLVDYNQNAWGRTLASVYSVR
                     PKPAATVSAPVTWQEIERGLRMENFTVQTVPARLKKIGDLWKPLLLQTGRTRLENFL"
     gene            25898..26890
                     /locus_tag="VN788_12230"
     CDS             25898..26890
                     /locus_tag="VN788_12230"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HXU21340.1"
                     /translation="MEALPAPKLPEGREWHYEPKWDGFRCIAFRDGKNIYLESKSGKP
                     LTRYFPELIAALTSLKANRFVLDGEIVIPVNGNLSFDDLLMRIHPAESRIRKLSQATP
                     ATFIVFDLLVDERGKKLVNLSLRERREALEEFAKKFIGENSSIQLSPMTRDISLARKW
                     FHMGTGLDGIVAKRDNLPYQSGERTGMQKIKTQRTADCVVGGFRYLEKKPLVGSLLLG
                     LYNQQGLLDHVGFTSSIHSEERPALTKKLEKLIKPPGFTGKAPGGPSRWSTKHSLEWQ
                     PLAPKLVCEVQFDHFTGGRFRHGTKFLRWRPEKSPKDCTMRQVQRENRSALDLL"
     gene            26907..27095
                     /locus_tag="VN788_12235"
     CDS             26907..27095
                     /locus_tag="VN788_12235"
                     /inference="COORDINATES: protein motif:HMM:NF040444.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HXU21341.1"
                     /translation="MARYGKSAQKSVKRAMHKKKHGTLKSGKRGRGGKVKSRKQAIAI
                     GLSEARKKGAKVPRKKKS"
     gene            27384..28253
                     /locus_tag="VN788_12240"
     CDS             27384..28253
                     /locus_tag="VN788_12240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU21342.1"
                     /translation="MHQMSQPTAELLRQLVAHLRQNRTQLREEWARRITEAKLLTAMT
                     KEEIFAEATSVYDNYVEALETGTFEALQAYARNLSERIIPRGVETHEVVGIVLLLRDV
                     LARSLFAKYQNDFALLNRILDAYEPAANRIANTVAVGFVQERERVIREQQEAIRVLST
                     PVLQVRERLLILPIIGVIDPQRARQLTEQLLRGIRTNRAKVVVIDITGVPVMDANVAN
                     NLVQTVEATRLLGATVIITGLSPEIAQTLVTIGVDLGKMNTVGDLQGGIEEAERLLGY
                     KVVPLTDNAELRT"
     gene            28258..28644
                     /locus_tag="VN788_12245"
     CDS             28258..28644
                     /locus_tag="VN788_12245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU21343.1"
                     /translation="MEVPILKQGPYLIASIQSALSDADLVQLRDALVEQVGRLRSRGV
                     IVDVTALDVMDSFATRTLRDITFMIRLRGAETVIVGIQPDVAFTMVQLGLKLEGVPTA
                     LDLEEGIAFLSKKDNGANKEATPRGR"
     gene            28610..29107
                     /locus_tag="VN788_12250"
     CDS             28610..29107
                     /locus_tag="VN788_12250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005373113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HXU21344.1"
                     /translation="MRTRKLLLVDGECDPVDAHAQPDRFTANGRDRVLEEIAVQIHCD
                     TDIVAARQKGRVLAASLGFSSSELTLIATAISELARNIVLYAKQGEIVLRLYERGEQR
                     GVMVRARDNGPGIPDIRRALQGGFSTSRSLGLGLPGVRRLMDEFEIDSRVGQGTTVTA
                     RKWRR"
     gene            29095..29736
                     /locus_tag="VN788_12255"
     CDS             29095..29736
                     /locus_tag="VN788_12255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXU21345.1"
                     /translation="MEKIKPLLLDCGVAASTLPGQTETGDRHVIAHFKNGAMLAVVDG
                     LGHGPEAAAAAKIAAETLERNAKESVIQLMRLCHESLKPTRGVVMSLASFNFADSTLT
                     WLGVGNVEGVLVQRDAYGTVAHEVLPLRGGVVGDRLPPLLASIVPVSRGDTLIFATDG
                     IRPNFTDGLKPESVQATASTLLKNFARGTDDALVLVARYSGSRKEVPGGRAAS"
     gene            29717..30619
                     /locus_tag="VN788_12260"
     CDS             29717..30619
                     /locus_tag="VN788_12260"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21346.1"
                     /translation="MDGLQVDYERALQTYLREGGESSLKQAYELGRRALGEGKGILDM
                     IGVHQQTLERILDGQPAGDLVHRAGDFLTESMSPFEMTHRAFGEANETLRRLNESLEG
                     ETRRIARALHDESGQLLAAVHIQLLQLSRHLAPSAQASLRDIEALLEQIEQQLRNFSH
                     DLRPTVLDDYGLVPALQSLAERFSKRFGTPVSVDSKPMPRMTKTMEAAMYRVAQEALN
                     NVARHAKATRVYIRLWMSPGELHCRIRDDGVGFDPAALPRASKNALGIIGIRERLNVL
                     GGSLEIQSAPQQGTELKIEVPVED"
     gene            30623..31255
                     /locus_tag="VN788_12265"
     CDS             30623..31255
                     /locus_tag="VN788_12265"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU21347.1"
                     /translation="MPIQVLLADDHQIVRQSLKVLLEKEGLKIVGEASNGQEAVRIAE
                     SMHPDVAVLDVSMAVLNGIDAAKEIQKVSPQTKTIFLTVHDEDPYLLDALRVGAKGYV
                     VKTHAAENLVQAIREASRGGVYLSPEVSRAVVQAYQNKTELSSEPLSPRERQVLQLIA
                     EGKTTKEVAGVLNISVKTAETHRTRIMEKLDIHETAGLVRYAIRRGLVQA"
     gene            31457..32680
                     /locus_tag="VN788_12270"
     CDS             31457..32680
                     /locus_tag="VN788_12270"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU21348.1"
                     /translation="MNASARVMELDGSVGTRELRGELNSPSSLDHIPALEELSATRSP
                     AMAELRHQIDRVATVDVPVLLLGESGAGKEFAARAIHARSLRNQRTFLKINCAALPSE
                     LLESELFGYESGAFTGAVRPKPGLFELCDHGTILLDEIGEIPPSLQAKLLHVLQDQQF
                     SRLGGRRIITVDVRVIAATNIDINKALEQKTFRTDLYYRLNTITLHVPPLRERREDIP
                     LFVDHFLDSLSVQLGCVPRPLSPRAMQACFEYSWPGNVRELKSFICRHLVLQDDDALI
                     AELLSASNPAVSPQEGSTQTSGLKPRIASIRAEAEKQLIEECLRKSRWNRTETARALH
                     ISTKTLWNKMRQYGITDSSRRGDIPNGERKRKFSTVDSQAGGDVESHFFGSMDKVRPI
                     EFAKRAPVSASTGTR"
     gene            32796..33386
                     /locus_tag="VN788_12275"
     CDS             32796..33386
                     /locus_tag="VN788_12275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21349.1"
                     /translation="MKPMPSTDAPRPFALISPPARLSLVPEFAESTPKFSVASTESPE
                     PNRFSANTPSAACESAAEHARAIAEARQTLANQQAEFARRLAKQLDSFNRQIASQLEN
                     LSGQVIQRFAEELNAHAGHALNALMSDWAEQNRALVDTECNRHLDQFSARLQSLSTAH
                     LEIYRKEMQNLSSNLKSRLRSVAHALQDVGPTSHRS"
     gene            33665..33844
                     /locus_tag="VN788_12280"
     CDS             33665..33844
                     /locus_tag="VN788_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21350.1"
                     /translation="MSLFSLREDADGGSQGEARGELVKGVSSEDPYGGRESYPIDLLY
                     ALREPPIVFGEPYLT"
     gene            34263..35702
                     /locus_tag="VN788_12285"
     CDS             34263..35702
                     /locus_tag="VN788_12285"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXU21351.1"
                     /translation="MRSCDLPAAILHSAEDAIIGLALDGTIVSWNPGAERLFRFTPHE
                     AISQPFSLFLAAEASTELLHLLAELPSGRRFSPRELECTRKDAGPIFCSLSVSPIVSP
                     SGEIVGASLIAREAGSFHQSEHELRHANKKLSESVRNLEQRNREMTIVQQLSTLLQTC
                     LSAAEAHDVLGQSVPKLFPSCSGALFEWNNSVKLLEATVKWGEASVGEAVFNPEQCWA
                     LRRGQVHRVADSSAPVLCAHLSRLSSTNSICAPLLASGETLGVLSVQGSPYELSLPKD
                     VQQRLLSARQQLVEMLAGHVALALANLKLRESLRAQSIRDPVSGLFNRRYLDETLPRE
                     IHRASRDGRDLGIIICDLDNFKSFNDTYGHAAGDTLLKSFGDLVGKVVRAGDIACRYG
                     GDEFVLILLDTSLETAKRRAELLEREFSRTVIQYGDMFLNPGALSLGVSAYPVHGANA
                     GALLRVADEALYRAKAAGGKRVILGQLVD"
     gene            complement(36050..36556)
                     /locus_tag="VN788_12290"
     CDS             complement(36050..36556)
                     /locus_tag="VN788_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008930153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPP family protein"
                     /protein_id="HXU21352.1"
                     /translation="MRSTYVFVNGFVTIALLALLAFFSRNPFVFPSLGPTAYLLFFSP
                     LGKTSSPRNTIIGHAIGLLCGYGAFVVTGAGALPFGVHPGIFWPRIVASALSLSATGA
                     FMVLLDVSHPPAGATTLIVSLGIISKPRELVIIEVAVLLLVAQALVINRLAGLPYPLW
                     SARNAING"
     gene            complement(36667..38334)
                     /locus_tag="VN788_12295"
     CDS             complement(36667..38334)
                     /locus_tag="VN788_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HXU21353.1"
                     /translation="MVRFDAAHQAVHYPPDRRFRIWIRPSILIGIVIAIAALVAAAWI
                     QVAVAGMPGVPAVPQIYPNNFAGPHGFPVWVRYCHFFNFLFVMMLIRSGLSILVDHPR
                     LYFNDGCTPGSEWIRFTPLKVPKDRIWTAKDDARYISPLIGTPGYRHTIGLARVWHFI
                     DVHGFIATGIIFIVMLFETAQWQRIVPASPVVVLQAWNTFVHYATFHLPSEPNGFYGY
                     NALQQIAYFTVVFVFGPLAIATGIAMSPAVVNRFPWYARIFGGRQSARSIHFLTMFSF
                     LAFLVVHVTLIVMTGFARNMNHIVMGTDDQNPIGMYLGFLGIGVVVLSWVAAHYISWN
                     HPRGVQHALKSVTYPMQLITLNRLLPQQKYTQQQISPYFWPNGKMPIREDWKRMAETG
                     FADFRLKVGGLVNHPVELSLADIERLGEVEHITMHHCIQGWSGIAKWGGIPMKKLIEL
                     VQPKPQAKVVAFFSFGEALYGGPYYDTQSLENVLKPQCVLACRMNGQLLPQLYGAPLR
                     LRVENQLGYKMVKWIERIEFIESEKLLGKGEGGKNEDDEYFDLLPNI"
     gene            38728..38803
                     /locus_tag="VN788_12300"
     tRNA            38728..38803
                     /locus_tag="VN788_12300"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38761..38763,aa:Ala,seq:tgc)
     gene            38864..>39059
                     /locus_tag="VN788_12305"
     CDS             38864..>39059
                     /locus_tag="VN788_12305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21354.1"
                     /translation="MHTVHECVTILAYQSYASWAWTLLPPSAMLELAGITAFALNIFG
                     TFLLQPSRAQHRQLIIGIPKA"
BASE COUNT         9017 a        10810 c        10571 g         8661 t
ORIGIN      
        1 tgccggaagg aatgccacga taccctcgga atcccaatgc ggtcgcacgt tggagcgagc
       61 accatcttgg ggagtttcaa cagtcactga agagtgtcga tagccgcggc gttcgctttg
      121 tcggctctgg cggggatgac aactctcgct tcccttgccg gcttgagctg tgggagtttg
      181 atcttccacg cgaggccgag cctttgaagt gccacaatgc cgtaccaatt cagatcgaac
      241 tcataccagg ccaggccgtg acgcgccgac tgcggcgccg catgatgatt gttgtgccag
      301 ccttctccga aggtgagcat agcgacccag aagctgttgg tcgacatgtc gccggtgggg
      361 aatcgctgtg atccccacat gtgtgttgcg gaattcacca gccaagtgga gtggagaccg
      421 atgaccgtgc ggaaaaagat gccccacaat aggaacggcc atccgccgat tgcaaaaatt
      481 cccagaccga tgacgaccaa aggaatccag tgccatttgt tgatccagac gtggaatttg
      541 tctttgcgaa ggtcaggaac aaagcaaaga agctcgcgcg aggggttttg tatcgccttg
      601 ccggtcatga tccagcctat gtgagcccac aatccgccgt cttgcgggga gtgcggatca
      661 ccctccctgt cggtgttctg atgatgaacg cggtgtgtcg ctacccagcc gattggtccg
      721 ccttcgagag caagtgtccc gcacacggtc aggaagtatt cgagccattt gggagtttta
      781 tagccgcggt gagtcagaag ccggtggtaa cccattccga tgccgaggct cccagcgacc
      841 caccacagca cagccgccag cgcgagagct ttccatgtga agaaaagcag cgccgcgatg
      901 gcacccaggt ggaatgcaac cataaaaagg gttgtaatcc aattgacggg ctgggtaaac
      961 gttcggtcgt gctggaagat ggtggtcact tgataatcct caaggtcatg ctcaggagat
     1021 ggtcgccgac tgggacgaat tgggattccg ggtgattgac caatggaaga atcgaaagtc
     1081 gcatatttcg caacgaaacg gccgtacgaa gaccatcgcg agaattgctc gttcaacgat
     1141 gcccctgcgc cttgatcgac ggatttgttc gctgtgacaa atcgggcagt tcacaatagc
     1201 gaatctcctg agtcccaacc taatactgag gcatgggaac tcctactatc tgtctcgttt
     1261 tgaacacaaa cgactaaacc aatctgaggc tttgctttgc cgctggccgc ttacaggcat
     1321 gtttctgcgt cacaacatcg ctctaaggcc ttctcaaaag aactggccat cgctaatttg
     1381 aggatggcgt accttgaata attcagattc tcttcgttcg atccggggct agaaaattct
     1441 gctgctctgt agatcatctt gctatcgaag agcactaacg atccgtgcga ggcatcgcca
     1501 tatctgcacc ggacgtccta tccgtgcgct ccgcccaaag tgaaaggtca ggctgacgcc
     1561 gtcgagagtg accgaacgcg gctcatcggg ataattttgg cgccgaagac ggaagtcttg
     1621 cagactacca ccgccagaag ggccaagctg cacattctgc atgtccagcg tgctggtaaa
     1681 gccatcgagc cgcaaacgtc cgccccagag ttccacgagc ggaaggctcg accgtgtgct
     1741 aaacaaagtc ttaacttcgc gcatcgatga aaggctctcc aaatcctgca ggctgtggtc
     1801 acgcgcgtaa ggacgagcga gcgagtagct gaagtggaag acggattttc cgcgatttgt
     1861 agaggagcgg aagcgtactg cggggaccgg catcggtacc gttcgaagca cggcgctaat
     1921 cggcgtaatt gcctcttgcg ccgggacaat ttgtagcgca atcatttgtg ctgccgtggc
     1981 cacgttggca caaagaaaca tgccgagcag gaggatgaac tgtttataga ccttgcacat
     2041 cgctgcctct cgtatcgcgg gaccgtccct gcgttctcag aaagcgggcg acaccctctg
     2101 taaattcctg aagacttgac cctatgcggt acgcatttgg gagagaaaat tattctaatt
     2161 aaatcgcgaa ctcgccgtct gttctaatac gcacaccaca cctgttcttt ttcgcacagc
     2221 ccccggtccc tcgacgtatt agtctattca cgaatctgat tctccggagt ggtgaacgct
     2281 agctcctacc gagtcaaaag tgagaatcct gaccaaatct ccggtgctgt tcttggtcgc
     2341 gcttacggct tcatccgcgc tctttggcca aactggcggg agttccccgg ccaagtctca
     2401 gagtcatgcc agtgcgctgg atgcgcacca aatcgtggaa cggtcggttg cagccactga
     2461 gcggaattgg cacgcgcgcg acgactacac atatatcgag cgtgatgagg accagcgcct
     2521 ggactcgctc ggaaacgtga aatcggagaa tgtggacgtc acaaaaacca ttgtcgtcaa
     2581 cggcgctcct tttgcgcagc tcgtagaaca caatgggcgc cctccatcgg ctgaggaaca
     2641 gagcaggagc agcgagaagc tctacaattt ggagcacgaa acgccgcagg agcgaattat
     2701 ccgacttggc aaggacgaag agagcaagtc gtttcttcgg aacgtggtcg aggccttcga
     2761 cttccagctc gtcggtgaag ggttagccgc cggtaggccg gcctacgtac ttcgcgtaac
     2821 gccgcacccg ggctatcagc cgcacggcaa gtacggcaag atattctcca aggtggaagg
     2881 caagctttgg atcgacaagc agaatttcgg ctggatcaaa gtcgacggcc gcgtaacgag
     2941 agcattttcg atgggattgt tcgtggcccg catgcagcgc ggctcgcatg tcacgctgga
     3001 ggaaacctgc atgggtgatg gcgtctggct gccgaaacga attgaactac gcgcaagcgc
     3061 gagaatcctt ttcttcaaaa gcctcgacat tgaccggatc ctgacatact cagactacct
     3121 gctcgcgacc gtaccatatt ccgcgagcaa gtagtcatcg cccggcttaa aaactaaggg
     3181 cgcaagcaat ttgcgtctag caatgcgctc gccgagcgcc acattccccg cactcactac
     3241 ccagggcttc catccaactc acgagtatag ccgtgttcaa aactgaacag acttcgtttc
     3301 gttaccgaca tattgtgagt gggaaggcgc gacctccaag ccggtcattc gcatatatct
     3361 tgggacagcg aggaggtcca gggggcttgg gcttcatgtc aaacgtggcg cagaacggtc
     3421 ggcctgattt gctgacctcc cggacagagg gtacggcgtc tcacagatgc tggcccattc
     3481 gtgtcctctt catccaccac gatgcagagg ttgtaagcga ctgtctgcaa gagctgaaaa
     3541 aggctcaatt cattgttcgt gccgacatca ttttgactat agagcagtgc gcggaacaac
     3601 tccgctccca ctcttatgat gtcgtggtgg ctggataccc aagcccgagt tgggaaggat
     3661 cccaggcttt gcaacttctc cgtcagactg cgcaggagac tccccttctc tttgtaacta
     3721 ctggcacagg aggtgaatcc ttggcgcagc taagcgctca tggcgctttc gattgcatcg
     3781 cgcgagagca tgtcgctcag ttgcccttgg ctgttcgtca agctctgaat gaaaagaaac
     3841 tccgtgagga gttggaatgt gcccgaaagt ccttgcggca ttcgcaatcg ctgtatcgag
     3901 cgctagtgga caatcccgca tacggaatat accgatgcaa cgcggaaaga aagattgtgg
     3961 aggttaacca agctctcctc accatgcttg gctattcaac gaaagaggaa ctgctggcgg
     4021 agaatgagct gacggccctc ctttctcagc tcggtacgag ctcaccctgg gcggggcgct
     4081 cccgcgaaac atcgcaaatt gaacctgttg aaacagagtg gaagcggaga aatggcacga
     4141 tgctgaaagc gaggatcagc ggacgcgctg ttttcgacga ccatggaaac tttgctggcc
     4201 atgaaatcat cgctgtggac atcaccgagc aacggacgct tgaggaccag ctacgccacc
     4261 aggcatcaac tgattcactg actggcttgg ccaatcatcg ccgccttgtc gaagcgctcc
     4321 acgcggagat ttgccgatcc aagcgaaccg cgcgggaatt ctcgctcgca ttgctggacc
     4381 tcgatgaatt gaaacaaatc aatgaccgat tttctcatct ggccggcgat cgagcgcttt
     4441 gccgactggg acagatactc acagactgct gccggtccat agacacggca gcgcgccacg
     4501 gcggggacga gttcgctcta gtcctgccgg agacaggcgc agcggccgcc tcactgctag
     4561 cgcggcggat ttgcgagctt cttgaaagag aggccgaaga gccggcactc tcggtgagcg
     4621 tcggaattgc cagttatccg aaagacgcgg ataccattgg aacgctgctc tatgcagcgg
     4681 acagagcgct ctatgccatg aaacgcaagc agccaagagc cgcccaatca ggcctaggct
     4741 caatccacaa tgtagcgact tgagaaagaa gggatccatg gatgaatatc gcttccgccg
     4801 acgcgattgg tcaaaagagc ctgagccgcc cccgaattct gaacagggaa ttcaacttgc
     4861 cctgagtgtc gagaaacaac ttcttcattc tatttctgcg tatgcgcctc ttcctgaagt
     4921 gctgaacaaa atctgtagtg ctcttgatta tcagatcggc aacgtggttt cggtgatttc
     4981 tctgccgtgg gacaacgcaa gcaagcgtgg cggaattgcc gttagcgcgg cggtttttgg
     5041 cttgcatatt ttctgcactg aaagtgttgc ggacgaaaac ggtgagctgc tgggattttt
     5101 ggagatgtac agttgcgttc cgcgctcccc ctcccctggc gagtgtcaat ggattaaatg
     5161 ggcgggctgt ctggctggcg ttgcgatcaa acgcagcatg gagacaggtg atcaacgtga
     5221 tcgtgctgtg cctcaaaatc agccagtgcg aggacggctg ctcaaacggc tggttttcat
     5281 ccgcggttaa tcaaaatgcg gagtgtccac aaggggacag acacccatct tctttgtgac
     5341 tattctgctt gtctgtgtaa ctttcttctg cacgcgagga gtcatcagcc tagtttccga
     5401 aaaatgaaac ggcggatgtt tttcgcgctc agaagcaagg agataaaaat atgctcgcgg
     5461 taatcgctct tgtcctgatc gtcctttggt tgtttggatt cttcgcattt cacataacca
     5521 ccgctttcat tcacgttgcc ctaattgtgg gcctgatatt gctcgtgttg catttcatgc
     5581 gaggcaggcg agcgagggtg taaacgttcg tcctccgtct tgcgttttcc ggccatacga
     5641 aaagttgggt tactcgtgca ggacgacgct taagagagaa gtgttgatct gaagaccgcc
     5701 gttgtactca atgaacccta gcttcttgaa tttgttcatg aaaaaattca cgcgcgaacg
     5761 cgttgtgccg atcatttccg ccagcatttc ttgcgatatt ttgggaagca ctccgtgcgg
     5821 ctggtcttcc ttaccgtaac gcgcaagcaa tagcagagtg cgggccagcc gcttctcgct
     5881 ggagttgaac agctggtcga ccaggtcttc ctcgattcgg atgttccgtg ccagcatgaa
     5941 agcgatgaac cggtccgaga gggcgctctg ctcgtgaagt actttgagca tttcactctt
     6001 ctcaatgacg agcagagtcg taggagtaat cgcggtagct gtccccatgc gaaccgactg
     6061 gcctgccaga cagccttcac cgaagaaatc gcccggtccc agaatcgcga ccactgcctc
     6121 tttaccggct tcactgataa ctgttagctt cacgccgcct tcctgcaagt acattacact
     6181 gtgggctgcg tcgccctggg agtagacgac ttcccctccc ttaaactcct taactctcct
     6241 cgccactcca gccgtatcaa gaaaaactcg caaatcgaaa ctggagttcc tctttggaat
     6301 tagtttggtc tttcgagcgc ctgttttggg catacctctc ctcgatcgaa tttaggcacg
     6361 gcggccgccg cgtttgcccg gcgggctcgt tcttccataa tctttcgaac cagttcgagc
     6421 ggaacctgct cgaaatgggt cctcggcttg agcacgacaa ctccttgaga tcagctggat
     6481 gctaacaggc caaaagccat acgggtaaca tcgtttggaa atcgcattca cgcataatcg
     6541 caggttaagt ccgtccctta gccgtcgcca cgcggggggg ggcgcgctcg cgtcacgact
     6601 tgttttttta attgcagaac tcgctacgat gagatacgat aacggctcgt ttgtttggcg
     6661 tgtcattacc gggaggagta tatggaacgc actcaaagca aaacgccacc tcgccacttt
     6721 agaacgatgg tgcaagcgga tgtgccccag ggccgaaacg gaaaacacaa aaaaattgtc
     6781 accacaatcc tcaatgatct cgatggtcta aaagctaatt ctgcgcttaa ggtcccgtta
     6841 gcggaattgg cggaatcaaa ggagagggtt cgttccgcct tgagtcgcgc aactcggaaa
     6901 gctggccgga aggttgccac tgccagcgac gcaactttct tgtatgtgtg gaacgtaatc
     6961 gagtagggcc accgtccggc gtttgccgct gacctattgg gcttgaccgt gatgttcggc
     7021 agccgcactt tgtccgaagc tgaactccaa tacaatcctc aggattttgg ggaaatgttt
     7081 agcggtttcg gagaactttt cgtaacgact gatgcacttc cagtccccca tcgtagctta
     7141 tgaatccctg tttcctgaat cgattcatga ataaattgat tcgcgggcga gttgtcccga
     7201 ccatctccgc cagaacttgg tgactgagaa tggggagctc ggtaactggt ggatcgctcc
     7261 tttccaactg tgcgagccgc agaagcacgc atgcgagccg ttgttcggct aggaagtggc
     7321 agatgcgcca cgtgatcctg gtagttattg acgctggata gtagataagc caccagagaa
     7381 ttcgacgtct tatttttctt gcgaagcatt tcgagcatct ttgccttctt gatggtacgt
     7441 atggagctgg cagtcaacgc gatggccgtg gacgtacgaa ggggatagcc agcgaggcaa
     7501 aggtcgccaa aaaaatcatt tacgccaagg atcgcagtaa cagccggtgg ttgatatttt
     7561 gtccgagtgg ttaaccgcac ccctccttct tgaatgtaga aaagcgtgta cgctggcgca
     7621 ccttgtttgt aaatgtgttg tttgtcccga tagtgcgaga ccgtgccgcc caagctggcc
     7681 ccgaagagaa actaccaagc ggcggagtca tttgttttct tcgtttcata agcagttccg
     7741 cttttgcaat tccaatgcgc gtgaatgcgc gcacatgatt ctgcgaactt aacaaactcg
     7801 acggcaagag gctgtgcaaa agcggaccct accggtggct ggggcttact cagcagttct
     7861 aaagtgatgg ctggggacgg tcgctcgaac ggaagtgccg ttgtggcctg tttcaatctc
     7921 caggctgccg ccgagttgct taactctttc ccgcatcccg gttatgccga tgccgacggt
     7981 cttgattcgc gatgaatgat ccagcacccc atccggtatc ccctgaccgt catcgctgac
     8041 cgttatggtg acgccatcca ggttctgctc aatctttacc ttagcctcgc tggctttcga
     8101 atggagatgg acatttgtaa ggcttgcctg cacgacgcgg aagatcgcaa gttcggcttc
     8161 ttccgagagt cggcgcaatt catgacttat ctccactttc acatgcaggc cgtggcgctg
     8221 gttgattccg ctcacatacc actggattgc ggtattcaat ccgcattcat cgagcatggg
     8281 tgggtggagc aggtaggaaa gggttcggat ctcatctgaa atgcttccga tgagaaattg
     8341 gcactgcaac agaatcttgt tgcgcgactc cgcagggagc tcatccgtga tcatggacag
     8401 atcgatcgcc aagagagaaa gcttttgtcc ggtgctgtcg tgcaggtcgc gcgcaatgcg
     8461 acgccgttcc tcatcttgtg actgataaag ccgcctggaa aactcgcgca gttcttcttc
     8521 cttatggtga ctttcgatcg cgattgccgc catatgaatc acctgttcca tcagatccag
     8581 gtctcgttca ccgggccgct gggaggttgg ataaaataag atcagagctc ccaggatttc
     8641 tttgtctttc gaaaaaatgg gcgcggacca tgccgcttga ataccctggc ggagagctaa
     8701 gtcccagcac gcggcaaacg agggatcgct tctcatgtcc gccagtggga cagattcttc
     8761 cgagtgtcca gccgccccat ggaaactgtt ggagggcact ttcaaacctt cgaacgcgtc
     8821 gttccagatc ttggtaactt tgggtccggc cgcaaggcgg agatgcgttc catcgggatc
     8881 gggtaaaaga accgtgggaa ttacgctcgg tgatttggca tggataaggt tgcacagttc
     8941 attcaagact tccatcatcg gtgcaccttg tgaaagcatt ctgagaacgt tcgtttctga
     9001 tgtcgctagt tggtgagcaa gagcttgagc catgttggtt ctccttcccg gaagactgaa
     9061 cacgtcttct gggtaaccga ttcgaatttg ctatctgcac aatgagaagg gcagaacggt
     9121 acacgcatgg gtgttgaggc gtctgttcaa ttggggacac acctcgttta gtgcgtggca
     9181 agccggcgct tttggcattg ggatgaaatt cttagccccg tcgcgggctg ctatagcgtg
     9241 atgtgtacac aactgaacag acatacgtcc gtgcaacgcc tagggtgaga gcgcaagact
     9301 caaagcctgc ttgattacat gggaggatcg tatccatgaa aagcttattc gtagtggttt
     9361 cgttgcttac cgtattgtgg tggccttggt cttctggcaa gaaatatcgc atgacggccg
     9421 acgccagtgt tccggccgcg agcgggactg ttcaagctca gaaagataag gccaatggaa
     9481 acacgaacat ggacataaaa gtgaacaact tggctgatcc cacgcgtcta actccatcag
     9541 agaacgtcta catcgtttgg gttcgtccaa acggcggggc gccagtgaag gagggggcaa
     9601 ttggggtgaa caacggcctg aggggtgaat tgaatgtggt cacgacctca aaggatttcg
     9661 acgtttttgt taccgcggag caaagcgaaa gcgtgatcct gccctctggc gtcgaagttt
     9721 tgcgggcaca tgtaagctca tgatacagaa agacagctgg cttagtgcgt gacagaaccg
     9781 ctgtcttgga attgggctca acttcctcgt ccgatcgcgg gctgctaaaa caagcaggat
     9841 gtgtatagaa gtgaacagac acgcgtctca gaaacgtcca agatgacatc gtagaaaagg
     9901 acttttgtgc cggcaagtca atccgcttca gcttcatttt ccacggcccg catcagggct
     9961 gaagacagcg aacgacacgt tactcgctcg gcctctggat aagtagtcca gcccttcgta
    10021 gcgttaaagc gtttcattac gttccgcacc cgatgagcca agctaggagt gtctcgaagc
    10081 agatacagga agggggaaag gccatgcctc tcattcaaat tatcgtagcc ctgatcgtag
    10141 tgggtgttct gctttggctg ataaaccgtt tcgttccaat gcagcgaacc atcaagtcca
    10201 tcttgaacgc agtagtggtc attgttgtgg tgttgtggct tttggatgta tttgggctgt
    10261 ttcattctct cgcgcgcatc cacgttggga cgtaggcaag tcgggatgaa cgtggggtgg
    10321 aactcccggg tcacaagtct cattaacagt cggggaccat cgtgagctag ggcagcgggt
    10381 gcatcagcat gcagaaattg gaggcaacgt catgaaacga attggagcca tcagcaccgc
    10441 agcattgttt ctgctcctgg gaattgctgc gccagcttac gcacaccagg accaacaaga
    10501 gaaaccgccg aaacaggagc agcgagccaa accctcaaag catcagcagc aggccaaggc
    10561 cgccgcagga cagcaacaat ccaagaggca acaacagcag accaaggcca atgaccagca
    10621 acagcagcag caagccaaga ggcgacatca gcagaccaag gctgatgacc agcaacagca
    10681 gcaggccaag agggagcaag gtcagcggca acagcgcgcg gggcaaccgt ctcgcatcca
    10741 gaaagttaat atgcaaccgc tccaggtcca acgccagcag gaacaacacc aacagcgcgc
    10801 ctggcaagag catcgcgcaa ataactggcg gtccgagcac cgcacctggc agcagcgcgg
    10861 cggctacaac ggctaccgta ttccagacga ccgttacaat cgctacttcg gccgggaaca
    10921 cgcgtttcgc atctacagtt accctgtggt ggtggttggc ggataccctc gctttcagta
    10981 caacggcttt tggttcagtc ttgccgaccc gtggccggaa tactggtcca acaactggta
    11041 cgagaacgat gacgtttata tcgagtactc cgtagacggc tattacatgt acgacaccag
    11101 atatccgcgg gatcgaattg cggtcagtgt ctacgtgaac tgaggttcac cggagggcgg
    11161 tgaaactaaa gcaatccagg acctcagcgc ggaaacttct cgagaattag accgcagctg
    11221 aggcagggtg cctgagtgtc ggggataagt gcgcaaagga gacagaaacc atgcttggaa
    11281 ccatattgat tgttgtcttg gtcttggcac tctttggagc atggccgcga tggtctcaca
    11341 gccgaaattg gggctatggc ccgaccggcg gcttaggcct cgtactcgtc attgttgtca
    11401 ttctcttgct tcttggtcgc atttagattt tgcgccaacc gcggaggtag tcgatggatg
    11461 agtctaggcg gcgtgtagtt agattcctcg agaaagaagt caaaacctat aaggcgcttt
    11521 ctctattctt gtcgaagaag ggcatcaagg aacacttaca agcaggagag aaagaagctc
    11581 tgattagtcc gacattctac aaagaaagaa taaaggaagc gaaaaagctc ataaatgaac
    11641 tccgaacgcc gaattagacc gcgagcgtct tcgagcggcg ctctaagtgg ctggtgcccg
    11701 tggggcatcc gcatcgtcac cttcctcctg ctggcgatca tgtgcagcag tgcttcgttt
    11761 gcatattccg ttctcaccca tgaggaaatc gttgaccttt tgtggaccag tgagatccgc
    11821 ccgcttctcc tcgagagatt tccgacgttg acagaagaac agctcaaaga ggcacacagt
    11881 tacgcctatg gcggcgcagt catccaagac ctgggctact atccctttgg cagcgtggaa
    11941 ttcagcgatt tggtgcacta cgttcgcagc ggggactttg ttcgagagct gctgtcgcag
    12001 agccgggatg cgaatgagta cgccttcgcc atgggcgcgc tggcgcacta tgcttcggat
    12061 atcgccggcc atcccgccgt caaccgggcc gtttccatcg aatatcctaa gttgcgagcc
    12121 aaatacggca aatccgtcga gtacgcggag aatcacagcg cacatttgaa gaccgagttc
    12181 ggattcgaca tggagcaagt cgcaaaaaat cgttatgcct ctcagcaata tcacgatttc
    12241 attggattcc aagtgtctaa accactgttg gaacgggtct tccccgttgt ttacggcgta
    12301 aggttggaag aggtgctgcc tcgcgaggat ctcaccatcg gttcctatcg ctgggctgtc
    12361 agccggatga ttccggagat gaccaagatc gccctgcgaa ctcacaagaa agatatgatg
    12421 cgggagacgc cgaacttcgc aaagcgtaag ttcctctacc gcctgtcccg ctccgattat
    12481 gaaaggcagt ggggtaagga tttcaagaag gacggattca agactcgtct gttttccatg
    12541 ctcttgcgat acatgccgaa aattggcccc ttcaagagcc tggcgttcaa caatcccaca
    12601 gcgcagacag agaatttgta tttcaaaagc atcaatgcga gcgtcgacca ataccgcgcc
    12661 tttctcgaac aagttcgttc gggatcgctc gaactggaca atcgtgatct ggacaccggc
    12721 aaggaaaccg ggccagcgga atacacgctg accgatgaaa cctatgcaaa gttgttgggt
    12781 cagttagccg atcaaaagtt cgatttaaca tccgtagacc tgcgggacaa tattctgcac
    12841 ttttattccg atctttcttg tcccatcgaa acgaaaaatg acactgtccg ctggcgaagt
    12901 ctgctgtcgt cccttgatca gttgaagttg gttgcgccga gtcggattgt cggcgtgaaa
    12961 cgcgcaaact gacccttcat ttattatggc tctgcggtcg aatgatccat tcgagatctg
    13021 tcgctatcga cgatggctgg acgtgcgcgg attggcggcg agcctgcaag taattgcaaa
    13081 atgtgtgctt aagtggacag actagttttc ggggcccatc taacataatc acgcaaagaa
    13141 tttctcgcca tggaggtaac aatgaaacga tatttaataa gcgcagcgtg tttcgcgctt
    13201 gcagcaggtg tagtgtacgc cgcgcaattg aacaaggaac aaaaacgctt ggagagttgc
    13261 gggaccgtga tgcaggaaat catgggcacg cccgagaaca tcccccaaga ggtattggag
    13321 aagacggact gcgtaattgt tttcccctct gttctcaagg cggcgttcgg gatcggtgga
    13381 agctacggtc gcggagcgat ggtgtgccga gcaggcaaga atttcaaggg accgtggggt
    13441 gctccagcga tgtacgcgct cgaagggggc agcattggct ttcaaatcgg cgggcaagca
    13501 accgatctca ttctactggt tatgaatgag aggggagcta gttcgattct caacagcaaa
    13561 gttaaactag gagccgacgc ttctgttgcg gcaggccctg tcggacgtga tgcgtctgct
    13621 gacacggatg cgtatctccg atctgaggtt ctcagctact cgcgttcgcg cggccttttt
    13681 gctggcattt cgctcgaggg ttccacgctc cgccctgatg gcgacgccac ggcagatgtc
    13741 tatggccaaa aactcaccgc tagagaaatc gtgttaggtg gaaagattgg aattcctgca
    13801 tctggccgtc atttggttgc tgtgttgcag aaaaatgcac cgcacaatga atcaaaggaa
    13861 tcggcacagc agtagcaaag caggcccgaa gactcggtcg taagttcggg cctccttgtc
    13921 tgtacatagc ggaggggccc tcgaaactcg ccacccgttt tgttgcgaag gcatggccag
    13981 ctccgagggt gagcatcgga cggaactgga tgaaagccga cgatatttcg tggcccccat
    14041 atttatgcaa cccacgaacg atgtcatcga gaaagaaaag ggaccatgtt tgacagggat
    14101 caagcgctgg cactccaaga acagattcgc gtccgtgctt acgaaatcta ctcaggacga
    14161 ggtcgcgcgg acggtaaaga actcaacgat tggctgaccg ctgaaagaga attgactgaa
    14221 caaagcagcg ttgtcatgcg aaaaacaatc atcgcaagtt aatccggcgc gaagaatact
    14281 cgccgatgtg ttcgccgtat gctggtttgt ttcctcggag gtcgttcgga tctcggcgat
    14341 tctttgtgcg gatactcagg atacaaggct gtacggactg catgaccttc ttcgggatct
    14401 tcaaggcggc cgtgttttcc ggaggcgatt gaacgtgggc taaacgatct gtgtcgcaac
    14461 tacaattcgt ggctacgaat tgtgtgaggc cgatctatct atatgtcgcc gcacgcaaga
    14521 atcaagtcgc gaaacaggct caacaagtcg cgggctgcgg atgcccatct gccgtgtttc
    14581 gatcccttgc taagagtcac ctgtgccgcc ggtcgccact gtgttccgga tggcccttgg
    14641 catccagcca ggtcaggagc gagcggatgc taccgtgcgc cagagcaaca cttgaatcag
    14701 cggcaccata atagagatta actgtgtctc catctcttgc cacggtgtag ccacacggaa
    14761 atactacatc ttgaacatct ccctggcgtt cgtactccgc ctccggcgca aacatccagg
    14821 agtctccgcg gagcaagcat ttgtcgggct gctcgagatc aaagagggcg aggccaagcc
    14881 ggtagatgga gctcgaaggc gtcctgcgta cgccgtgata gatcatcagc cagcctctcg
    14941 aagtttcaat tggaggaggc gatatgccaa ttttgttggc atcccaccac gcgccgcgac
    15001 gtgcttccaa catgagtttg tggtttcccc aatgatgcaa atccggagag taggaaatcc
    15061 agatatgtgc gccgagaggt gtcatcggac gatggaccaa tgcccaaaaa ccacctatcc
    15121 tgcaaggcag cagtgcggcg tccttgtcgt caggcggcat gatgacacca aatctctcga
    15181 agctgcgaaa atcctccgtc agagccaacg acactcccgg accaccacga gagtaggagg
    15241 tgtacgccac cgcaaatttc ttgagttccg gcacataggt aatacgcgga tcttcgatgc
    15301 cccatacttc ctctgggtat tgcttggcgt caggcagcag agtcggttca gggtcgatct
    15361 gccagccgtc gacgccattc agcgagcgcg cggcgcacaa gtgcgagagt ccccggcgat
    15421 cttcaacgcg acataacaaa agggttgtcc cgtcccgcag caatgttgcg ccaggattga
    15481 aaacgctatt catcgagtaa ggccaatctt ttcgcgtcag gatgggattg gcaggatgcc
    15541 ggagaaatag cgtttcatac tgtgtcatcg taggcttcac agagagtctc actgagagga
    15601 ctcggcaaaa ctgggcccag gctattaaag aaccaccctg ctcaaacctt cttccttatt
    15661 cccccggctt cttccatctg gtgcatttcg agcagggcca tcaggaacga caaagtcgat
    15721 tcagctccct ggttttcatt tgcccggtca gggtgtaggc cgtctcgaca accgccagtc
    15781 gctgaatcat agagtgcaat ttgaagatcg ttgtctccca agaaccaatt gaacgcccgc
    15841 cacgcctctg tgcgccagag accgcttcca gttgtgcgat aagcctccaa gcaggctgag
    15901 actcctgcac aagcttccac tggttgttgg tcaaaacggg ccttttcgct attctttgaa
    15961 tagaaaccct gtgatccgat aggcacgaaa tgacccttct cctcgcaacg ttgaatcgta
    16021 accagccagt tcaacgctcc cagtcccgct gaaagcatct cttcgttcgc ggtgcgcatt
    16081 cccgcccgta tcagagcctg cggcaaccgc gcattcgagt atgcgagcac atcctcgaac
    16141 caattccact cagcggagtg gttggcgcga taggaattca acaattgctt tgccagcgct
    16201 tcagcggcag ccagagctgc gcgatcgccg gggaacgagt cgagatattc ctgcaatccg
    16261 agcaacgcga aagcgcacgc acgcggactc ttgaattcaa cagctgccgg aaccgcgatt
    16321 tcaaacaggc gcccagcagc tcctcgcaac ccgtcgttct tggatctgcc gaggacgctt
    16381 cccaacgccc ataaagcccg gccatggctg tcttctgaac cttccggctc ctgccattgg
    16441 cgttcgtagc tcagagaatt tcgaaatcgt ttggtgtctg gatcaaacgc aagccacagg
    16501 aaagccagat accgggagcc caagtcaagc aaacccgttg gcatgcgggc cgccgcccct
    16561 tccaagagtg tggttacaat caatgcgcgc gcgttgtcat ctgacgtgta accttccgga
    16621 tagttcggca ccacgaaaac cgcatgttcc actattcccg tctgatcagt cattcgcagc
    16681 aggtggtcca gctttatggc gggaagccgg tcgagcacct tcggtgtgtt ttgggctgaa
    16741 aatgtggcgc gtggagttcg caagcgttca ttatagacgt catcgaaact cttcatatat
    16801 ttctgcgcca cgcgtttcca taccatgtct ctggcgtaca aatacgcgcg cttccgcatg
    16861 gcatgccttc ccatctcgtc atccagaagg gcgattgttt ttgccgctat ggcgcccggg
    16921 tcatcaaaag gaaccagcgc accgcgttca tcactgagga gttcaatagc gtgcaggtac
    16981 ggcgtcgaaa tgatcgcctt accggcactc aaggcatacg caagagtgcc ggaaaccact
    17041 tgcgccttgt gcttgtatgg cgtgatgtaa atatcagccg cgccgatcaa ttccaccagt
    17101 ccttgagggg tctcgaagcg gttgcgaaaa ataacgttgt cctcggctcc cagctccttc
    17161 gccaagcttt gcaaggaatg ccggtacttg tcaccctcgc gccgcaggat atgcgggtga
    17221 gtcacgccgg aaaccatata tacaacgttg ctgtgccggc acaggatttc cggcaacgcg
    17281 cgaatgacat tctcgatgcc cttatttggc gatagcagtc caaacgtcag caggacatct
    17341 tttccttccg tgccaaattt atccttgtag aaattcggat ccgtaaacgg cagatcaggc
    17401 acaccatggg gaatcatgtc aattttgctg ctgggaacgt ggaacacttc ctgtaggatc
    17461 tccgcggaat gctggctcat aacgatcaag cggtctgata gggtcacaat ttccatcatt
    17521 actgcgtatt gatccggatc cggctgacga agtacggtgt gcagtgtggt tagaaacggc
    17581 atctgcaggc gtcgcagcag ctccaaaata tgtccgccgg cgctgcctcc aaagatcccg
    17641 tactcgtgct gcaggcacac gacatcggcg ttgctgaaat tcaggaagtc cgcggcttgg
    17701 tgataagacg cgacgttgtc ttcggccagc tcgaatcgca ctctggcggg ataattgtaa
    17761 ccctcttcgg tatcgtttac cggaagtgcg agcagctctg tgttgctgta ttcagcgtgt
    17821 atggcgtcgc acaaatctgt cgtgaatgtg gcgattccac agcacctcgg cagataattt
    17881 cccacaactg cgattcgagt gggaagcgcg aggcgcgctg gtgccggtgt cagtgtgacg
    17941 agtttctcct ccaacacggg tgattgcatt tcgatcagca tggcagtttc tgcaagagac
    18001 tccgtgcgag acattgctat tttcggtcct gacatttggt ttcctgggac tctgcacttg
    18061 cggagtgaat tgtttaatcg tgtaaaacaa ccgtcagcaa tgatctattg attttgattt
    18121 tgccgttgta ttcgatgaag ccaagctttc gaaatttgtt catgaagaaa ttgacgcgcg
    18181 aacgggtcgt tccgatcatg cccgccaggg tttcctgaga aactttcgcc agtatccgat
    18241 caggttgttc ttgtttcccg tagcgagcga gcagcaggag cgtgcgtgcc agacgcttct
    18301 cactggagtt gaagagttgg tcgatcagat ccccttccac ccggatgttg cgggcgagga
    18361 tatacgagat gaaatggtcg gataactcat gttccgaatg gagcacgcgc aacagctctt
    18421 tcttctggat ggcaagaacg gtagtcggtg tgatggccga cgtggtgccc atccggagag
    18481 gctgacccgc catgcacccc tcaccgaaaa agtcagtagg accgaacatc gcaaccacag
    18541 cttccctacc agccccgttc accacggtga atttcacgcc gcccttctgg atgtacatca
    18601 cggtttccgc aacgtcgcct tgggagtaga tcgtttctcc ccggcggtag tcgatgactt
    18661 ttctcgccgc acctgcggta tcgagaaaag cctgcggatc aaaagcgcca acactccgat
    18721 tattcttgaa tttctttcgt ttcatattca gctcaaacta ccaaccccta agaggctcca
    18781 cacccaactt atcaagaaag ggtttcgcca gactgtataa aacagaacag tgctcattgg
    18841 attctctcca ggatgggata ctgcaccgcg agtaagcgta ggatcgtgat cttcagccgt
    18901 tggcctgacg ggtagtcatg gcctacgtcg attctggaat aaatgatagg gtcgaatgcg
    18961 tttttatttc aatcgctcgc tgtgtcctaa aagggacaga cagacctagc ttttctcgcc
    19021 cattatggtc agagtccaaa acatgcgcaa cgttcctcct gatgaataca acggagcagt
    19081 tgtctatctg caataccacg gccatcgcat aacgccggtg gagagtttcg cgagcagcga
    19141 tcctgcaacg ccgggtaccc gcgtcggcag aaaaacctta accgccgagg aggttgtatc
    19201 gcttgcagtc aacaagggct gggtactgcc ctagatttcg cgattttcca gaaggcaatc
    19261 tcgcatgagt cgtacgaggc aaaagacctg aatgcaagtt cgtttcaggt aaccatggac
    19321 gcatggctgg aatccgaccc tgctatggct ggcttgttgc aggccaagtt gtcaggtcgc
    19381 aagtcagtct ggagcgtgcc atcaagtccg aaaactggcg gctaagtaca aactattcgg
    19441 cgagcccagt cctgaccctc cgtacggcat ctttggcccc aaagctaact agggctcgtt
    19501 ttttctttct tcctctgccg gttgttacct cctgaccagc gccgtgtggc ccttttttta
    19561 ccgtcaagct gaagctgttt ttcgaaagca tacgcctctc gatgtttatg cataggcgcg
    19621 attgagccct ctggttgcga ccggccgcgt gttatcgttc gcgccgtacg gctggtgagc
    19681 ggttgaaaac tgagcgggtc tctgaggttc tgcagttccc ggtaataata aagggcagtt
    19741 ggatgttttc ctgagaaaat cagccttttc aggaattcga tttcttcctc agtcgcagcg
    19801 ccactgagcg aagagttccg aaggaactct ttgaagccag cttcctcccc ggaagattcc
    19861 ttttcaacaa attcgaatct cttaggacgc ccaggagcca atctacggtt tagcacaacc
    19921 tctacggcaa aggtcaccgg gtcgatatcc caggattcga tcagcggctc caggaaagaa
    19981 acacagtttt ccatcgataa atgaaagacg cccgtgtcca gaaactcgag gatgagcacg
    20041 cgcatttgct cgtagcttcg gccactcagt cgagctacca cgcgcagcca cttttcactt
    20101 tctaattctt ccttggcgac ttttttgacc acgtcgagga gcttctgcgt gacaatgcgc
    20161 tccactgcgc caaaagggtg gctctcgaaa atcgcacgta cctccttttc ccggttgcgc
    20221 gcgcacttgc gaagaatcaa ttctcgaact tctcgattca atggtgcggg gggatcgagt
    20281 aagcctctcc tcaattcttg cttgcgctgg tgctcggcta gctcagaaag attaccggcg
    20341 ggaagtttca agaacttgcc catcttttcg taaatgtccg ttcggttcgg cgccggaggc
    20401 aatttcttgc gagtgagcaa ctgcgagata taggactcgg taactccagc ggcagccgcg
    20461 agatccctct gctccagccc caattctttc agccgctgtt tcaccgccag acaaacatcc
    20521 ataggagcct ccgctcgagt tcactcgatt aagttaacca gttaaaataa tcacagattg
    20581 tatttgacat gtcaagtgaa ttaacctatt ctggttaacg cactgaggga gtcttaaggc
    20641 aacctaacct gaatgtggtt atctgccgcc ggctcttgcc acaacaacct aggaggttgt
    20701 catgtatgca cgcaaagttt ctgtgcagtt gaaggcgaat agcgtcgcgg aattcaccct
    20761 gaggatggaa aaggacgttc ttcccctgct tcgcaagcag aagggttttc aggacgagat
    20821 cacctgcgtt gccccagggc acaaggaagc gtttggattg agcttgtggg aaaacaaaga
    20881 gagcgcggac gcgtacagcc atgaaccgta tggcgaagtg acgaagttcc tgacaggcct
    20941 gattgaggga actgcccatg tcgaaagcta tgaggtatcc aactcaacct tccacaatgt
    21001 ggccactgct gcagcaacgg ctaagaatta agggaaccgt tctctacgcg ggggtgggaa
    21061 cattctgccc cctcccgtcg ctgcgcaaga ggttcgttat catccgaaag tttcagcaaa
    21121 ggagcaatgc gatgtcgtta ctgttgtggg ttatggaagg tgtgattgca ggctggctga
    21181 tgggaaaggt tatggggagc gaagggcgcg atcgggtgat gaacgtcata atgggcatcg
    21241 gcggtgcggt cgcaggcgcg ttgatcattg ccgccattca catcgtccac ggaaaaatga
    21301 tttatacgaa cttggccgcg atttctggcg gcatgcttct agcctttcta tctcggtacg
    21361 taggtggaag ccgggagtac ggtcgaagca actgagcttg aaacttcgaa acggagagtg
    21421 cccggattcg cctgctcatc caacttgaac gcactcgatg gaaaacgatg gggaacaagg
    21481 ctgactaaat ggccacgaaa atcatacctg gattcccgct gcgactttgc tcgtggtcat
    21541 gccgaccgat cgtgtccgtt gcagtaactc gcaaaatgat tcgctggttg gatgcagttt
    21601 gcctcaagag ccgatggaaa cctccaatga ggcatcggct ttgactcggg cagttgcttg
    21661 aaggagatgg tgaaatgaac attcgacgaa atgtactgat ctgcatcgcc gcgagtttgc
    21721 tctgcgttcc gttgggttat ggacaagatc tttcgaagta tcggaacttt tccctcggaa
    21781 cgagtttggc cgaactttca aagcaactga aggggaaacc ggcggatgcc caaataacct
    21841 atcagaatcc cgcattgatt caggagttga tctggttacc ggggcaatct tacccgtcgc
    21901 ccgcacaacc agagtcggtc caggagattc gattctcctt ctacaacggt gaactctaca
    21961 agatcgcggt gacttatgga aacctagcaa cagaaggctt gactgttgaa gacatggtca
    22021 cagccatctc ggcaaaatac ggcgccgcaa cgcggccagt tgcagtccct gctccaaccg
    22081 ccttggccga tactttcact aagcagcctg tcgcattctg ggaagacgct caatattcgc
    22141 tgactctttc ccgctcttct ctctcggatg cgtttcaact tgtcatgtat tcaaagcagt
    22201 tgaacggcca agccgacgcg gcgattgccg aagcggtgaa gcaagaacgc gaaggcgctc
    22261 cgcaaaggga aatcgcgcgc gcgaagaaac agatcgacga cctggaaaca acgcggcagg
    22321 ccaacttgaa ggtgtttcgg ccttaatgcc ggtcctcaat gatgaggtag cttgaatttc
    22381 ggaaggaatt gaaccggagg tcagagaaat gacggtgaac ataactcccc tgaatgaccg
    22441 cgtcctcgta cgcaggttgg aagagaaaga aaccgcgaca ggcggaatcg taatcccgga
    22501 cacggccaaa gagaaacctc aagagggtga agtaatagcg gtcggagccg gcaagatgga
    22561 aaagggccga cggatcccca tcgcggtgaa ggtcggtgat cggattttgt tcggcaaata
    22621 caccggcagc gacatcaagg tcgatgacca ggaatacatg atcctccgcg aagatgaaat
    22681 cctggcgaag ctcaacggaa tggtcaaggc ggcatccggc aagaagtaat tgtcttcgat
    22741 gagagcttga acgtggtgaa agtgtcgaac acccaggagg gtaaagtcca atggcaaaac
    22801 aaattgtaac tggtgagaac tcgcgtcagg cgattctgcg aggcgtgaac attctcgctg
    22861 atgcagtcaa gagatccgtt cgttctaggt tcgaatccta gcgggcccac cagatcagtg
    22921 aagcgaatag cagtcgtgaa ttttggatcc gcagcattcg cgggacagcg acgcgccgca
    22981 gagttgggcg cggcgcaaat ccgattatct gtgggcggga actcgaggca ttttcaaatt
    23041 tgatttctgc gcggtggccg tctcgaacga tgtatcgttg ctggcgtctt tgtacagcct
    23101 cagaacatcg ccagcccgct tgaacaaatc ggaaggaact tcgccgggct tgtagtcaat
    23161 ccatcccacg gaataattga cgctcatttc ctcacccgca gccttgatgc tcacactacc
    23221 gacgcggtcg gaaactctct tggcgccgcc gaggctgcat tcgggcagaa ttacgagaaa
    23281 atcgtctttt cccaaacgca cgcaaaaatc acatccgcgg ctggccttgc gcaaacggtt
    23341 tgcgaattcg gcgacaagct tgtcactggc atccttgccg aagcgctgtg tgatttctcc
    23401 caggtcgtcg agatgcagcg ccactatact caatggtaga ttccgccggc gcgcgcgagc
    23461 cacttcctta ccaagccgct gatcggccgc cgcccgcgtg tacagccccg ttaatgcatc
    23521 catttgggcg ggatcctgat tggcttcggc ggcgtctgcg cgttcgcttt gcgcatccgc
    23581 ttgttttctc gcgcgccgaa aaagccactg gcgatagagc agccatccat taaacagcag
    23641 cagcaggctc agtagtcccc agcgggcctg gtctgaagtg atttcgtaaa aatgttcctt
    23701 gtggaggaaa agcctgcgga aaaaggaaag gaacagccca gtggtcgaaa gcaccgtagc
    23761 cgcaaacgtc caaaaccaaa gccaccattc gctgcgtcca acccgctgta gttcagatgc
    23821 catctgacgc gcaccttgcg aagcagattc gatgggcttg ccgcaattcg cctagtgtac
    23881 cggttcggaa aagggcaaga ctactggttc aaagggcagt gtcgagcaat ccctacctgt
    23941 acgatcgttc gggtaagtaa atcgggacgg aaaatgtgct tttccggcgt gtccgctttc
    24001 aaccggtgac gcttccaaca acggcttcct tcgaaacggg cgacttcgat agcctcggaa
    24061 gtttcggcgg ccgcgggcca tgccgcattg aatttgcaag acaaacatag gtgagcggca
    24121 cgccatcgcg cacagcgcgc agcatcaagc cgccgcggct catgtctccg cgtcccgcaa
    24181 attctgtggg aaacatcagc ttcgcgccaa aaattccgag cagcggctcg gcgtattttt
    24241 gcgtggcgac actgccgagc aacaccactt cgcagcgccc aattctggcc gcgagccgcc
    24301 gcgcgtcgcg ctccaggggt tctcgatatg ttgcttcgcg ttcatcaatc gaactcgccg
    24361 ccatcgcctg caattcctcg atgcccacaa tcgtgtccgg tgtcatcaag ccatgcgatg
    24421 cggtaatcac catcacgccc actgcatttc gcggcggtcg cgcaaacgcg cgcgcatatg
    24481 ccagtttgcc gcgaaaatag agtccgctga caaaggtgaa cacctctccg agcggcgcgc
    24541 cgccgtcgcg taacttcagc gccatcgcgc aacgcgaaaa cccgccccac attaatttgc
    24601 cgcgtttgcc ggatagattc gccggtgaaa gcaggaaaat ccgagatttg tcgttgcgat
    24661 gattcatcgg cttgccacta cgatgatcca ttccattccc gatgagacct gcagactgcg
    24721 caatgtgaaa ttcgccattc atattaaact gaactgcacg gcgcgcccaa atcgcaacac
    24781 gcgctggaga taaaattgtt tgtgagcgcg ttgcaccctg aggtcaccag cgctggcgct
    24841 tcaccgtcta aaaagagagt gaatcgatgg cagtgcgagc caaatcggga cctacgcgcg
    24901 cccattttac gcggccagcc aaaacaaaac gttcagcgac gtgggcaggc ttcgagattc
    24961 cggccagcgc gaatgaagcc gacctccgcg tccccggcgg agttgtccac ctcaccaatc
    25021 tccggaaagt cttctggccg gaacggaatc tgaccaagag agatctcctt caatattacc
    25081 tcgacgtctc tccctggctt ctgccgcatt tgcgcgatcg cgccatggtg atgaagcgat
    25141 atccgaatgg aatcgcgggc gattttttct tcatgaaacg cgccccttcg ccgcggccgg
    25201 cctacattcc cttgtgttcc attgagcatc gctccggaaa catcatcgac tttcccgtcg
    25261 tcgagcacct cgcggcgctg ctttgggtcg tgaatctagg ctgcattgat ttgaatccct
    25321 ggtacgcgcg ctgtgatgac gtcaaccgcc cggattttct tcacttcgat ctcgatcctg
    25381 ttcccggcgc cactttcgat cgcgtcctcg aaacagcgtt gctggtccgc gattctctcg
    25441 ccgctctcaa aattaagagc tacgccaaaa ccacgggctc acgcggcatc cacgtttaca
    25501 ttcctatcat gcgcgggccg ctgcagaagc aggtctggtc tttcgcaaaa tccttttccg
    25561 tcgaactggc gaatcgccat cccaagctca tcacttccga ataccgcgtc gccaaacgcc
    25621 ctgcgggccg cgtgctcgtg gactacaacc aaaacgcctg gggccgaacg ctcgcgtccg
    25681 tttattccgt gcgtccaaag cccgctgcca ccgtatctgc gccagtcacg tggcaggaaa
    25741 tcgaacgcgg cctgcgcatg gaaaacttca ccgtgcaaac cgttccagct cgcctcaaaa
    25801 aaattggcga tttgtggaag cctctccttc tgcaaaccgg ccgcacgcgc ctggaaaatt
    25861 tcctatgact ctcccgctcc cacgcgacta cgctcccatg gaagctctgc ctgcgcccaa
    25921 gcttcccgaa ggccgcgaat ggcattacga accgaaatgg gacggcttcc gttgcatcgc
    25981 ttttcgcgac ggcaaaaaca tctatctcga atccaaatcc ggcaagcctc tcacgcgata
    26041 ttttccggaa ctaatcgctg cacttacgtc gctgaaagca aatcgttttg ttctcgatgg
    26101 cgagatcgtc attcctgtta acggaaatct ttctttcgat gatttgctga tgcgcattca
    26161 tcctgccgaa agccgcatcc ggaaattgag ccaagcgact ccggcaacgt tcatcgtttt
    26221 cgatttgctc gtggacgagc gtggcaaaaa gctcgtcaat ctttcgctgc gcgaacggcg
    26281 cgaagctctc gaagaattcg cgaagaaatt cattggcgag aattcttcca ttcagctttc
    26341 gccgatgacg cgcgacattt ctctcgcgcg caaatggttc cacatgggaa ccggcctcga
    26401 cggaattgtc gccaaacgag acaaccttcc ctaccaatcc ggcgagcgca ccggaatgca
    26461 aaaaatcaaa acgcagcgca ccgccgattg cgtcgtaggc ggatttcgct atctcgaaaa
    26521 aaaaccgctc gtcggttcat tgctattagg tctttataac cagcaaggat tactcgatca
    26581 cgtcggcttc acctcgtcga ttcattccga agaacgcccc gctctcacca agaagctgga
    26641 aaaactcatc aagccgcccg gattcacggg aaaagctccc ggagggccga gccgctggag
    26701 caccaagcat tcgcttgaat ggcagccgct cgctcccaaa ctggtctgcg aagttcaatt
    26761 cgatcacttt accggcggac gtttccgcca cggcacgaaa tttctgcgct ggcgtcccga
    26821 aaaatccccg aaggattgca ccatgcgaca ggtccagcgc gaaaatcgct ctgccctgga
    26881 tttgctgtga caaaaggaga aacgaaatgg caagatacgg aaaatcagca caaaaaagcg
    26941 tcaaacgggc catgcataag aaaaaacacg gcacgctgaa atccggcaaa cgcggccgag
    27001 gcgggaaagt caaaagtcgc aaacaagcaa tcgccatcgg gctttctgag gcccgaaaaa
    27061 aaggcgcgaa agtcccgcgc aagaaaaaat cctgacgtca atagctgcat ccttcgttgt
    27121 ggtcgtgaag agttaagcgg gctcttaccc gtaaaatccc ggaaaaacgc aagcgggtgt
    27181 ctacagttaa accagtaagg taagtttcct acatttccca gtgcttaccc gtattccctt
    27241 gtttttccct atttgccaca atctcaactt cttacagctg tcagaggttc gcccgagcgg
    27301 cggctcacga ttgcctctgg ctagaagcgg aggaacaccc gatggaacac gaagtccgcg
    27361 aggcccgcac caccttcggt accatgcacc aaatgtccca gccgacggcc gagctcctcc
    27421 gtcagttggt cgcgcacctt cgccaaaacc ggacacaact gcgcgaagag tgggcgcgcc
    27481 gcatcaccga agctaaactg ctcaccgcca tgaccaaaga agaaattttc gcggaagcaa
    27541 cttccgtgta cgacaactac gtcgaggctc tcgagacggg aacattcgag gcgctgcagg
    27601 cctacgcccg caatctttcg gaacgcatta ttccccgagg cgtcgaaacc cacgaagtcg
    27661 tgggcatcgt gctgctcctt cgcgacgtgc tggcccgctc tctgttcgcc aaatatcaga
    27721 atgacttcgc ccttctgaac cgcatcctcg acgcttacga gcccgccgcc aaccgcatcg
    27781 ccaataccgt ggccgtcgga ttcgttcagg aacgcgaacg cgtcattcgc gagcagcagg
    27841 aagctattcg cgttctgtcc actcctgtgc tgcaagtccg cgaacgcttg ttaattctcc
    27901 ccatcatcgg cgtgattgat ccgcagcgcg cccgccagct aaccgaacaa ctgctgcgcg
    27961 gcattcgcac caatcgcgcc aaagtcgtcg tcattgatat aacaggcgtg ccggttatgg
    28021 acgccaacgt tgccaacaac ctcgtgcaga ccgtcgaagc gactcgcctg ctcggagcca
    28081 ctgtcatcat cactggtctt tccccggaaa tcgcccagac cctcgtcacc attggcgtcg
    28141 acctcggcaa aatgaatacg gtcggcgacc tccaaggtgg aattgaagaa gcggaacgtc
    28201 tgctcggcta caaagtcgtg cctttgacgg acaacgctga gttgaggacc tgaattcgtg
    28261 gaagtcccta ttctcaagca gggcccctat ctcattgcct ccatccaatc tgcgctatcc
    28321 gacgccgatc tggtgcagtt gcgcgatgcc ctggtggaac aggtgggccg tttgcgttcg
    28381 cgcggcgtca tcgtcgacgt cactgcgctc gacgtaatgg actccttcgc tacccgcacg
    28441 cttcgcgaca tcactttcat gattcgtctg cgcggtgccg aaaccgtaat tgtgggcatt
    28501 cagccggatg tcgctttcac catggtgcag ttgggtttga aactcgaagg cgtgcccacc
    28561 gcgctcgacc ttgaagaagg catcgcgttt ctcagcaaga aggacaacgg tgcgaacaag
    28621 gaagctactc ctcgtggacg gtgaatgcga tcccgtggac gctcatgcac aacccgaccg
    28681 gttcaccgcc aatgggcggg atcgtgtgct cgaagaaatc gctgtgcaga ttcattgcga
    28741 tacggacatc gtcgcagcgc gccaaaaagg ccgcgtgctg gccgccagtc tcggattctc
    28801 ttcgagcgag ctcactctca tcgccacggc tatctcagag cttgcgcgca acattgttct
    28861 ttatgccaag cagggcgaaa tagtgctgcg tctttacgag cgcggcgaac agcgcggcgt
    28921 catggttcgc gcgcgcgaca atggtcccgg cattcctgat attcgccgag cgcttcaggg
    28981 cggcttttcg acgtcgcgca gcctcggtct cggccttccc ggtgtccgca ggctgatgga
    29041 tgaattcgaa atcgactccc gcgtgggcca ggggaccacg gtgacggcga ggaaatggag
    29101 aagataaagc ccctgctgct cgattgtggc gttgccgcga gcaccctgcc gggacaaacg
    29161 gaaacaggcg accggcacgt gatcgcgcat ttcaagaatg gagcaatgct cgctgtggtg
    29221 gatggcctcg gtcacgggcc cgaggcggca gcagccgcga aaatcgctgc ggagactttg
    29281 gaacggaatg ccaaggagtc ggtcattcaa ctgatgcgcc tttgtcacga aagtcttaaa
    29341 ccgacgcgag gagtggtgat gagtctcgct tccttcaatt ttgcggatag cacgctcacc
    29401 tggctgggcg tcggcaacgt cgaaggagtt ctcgtgcagc gcgatgcata tggaaccgtt
    29461 gctcatgaag tgcttccgct gcgcggcggt gtagttggcg atcgcctgcc gccgctgctg
    29521 gcctccatcg ttcccgtttc ccgcggagac acgctcatct ttgccaccga cggcattcgc
    29581 ccgaatttca cagatggtct gaaaccggag tccgtgcaag caaccgcaag cacgcttctg
    29641 aagaacttcg cccggggcac cgacgacgct ctggttctcg tagcacgtta ctctggctcg
    29701 cggaaggagg ttcccggtgg acgggctgca agttgattac gaacgggcgc tgcaaacata
    29761 cttgcgcgag ggcggcgaat cttcccttaa gcaggcttac gaactcgggc gacgtgcgct
    29821 tggcgaggga aagggcatcc tcgacatgat tggcgtgcat caacaaacat tggaaaggat
    29881 ccttgacggc cagccggcag gcgatcttgt ccatcgcgcg ggggatttcc tcaccgagtc
    29941 gatgtccccg tttgagatga cccatcgcgc cttcggcgag gcgaatgaga ctttgcgtcg
    30001 cctcaatgaa tctttagaag gtgaaacccg caggattgct cgcgccctcc acgatgaatc
    30061 cggccagctt ctcgccgcgg ttcacattca gctcctgcag ctctcgcgcc acctggcgcc
    30121 ttcggcgcaa gcaagcctcc gcgacatcga agctttgctt gaacaaatcg agcagcagct
    30181 tcgcaacttt tcccacgacc tccgcccgac ggttctggac gactacggcc tcgtgcccgc
    30241 gctgcaatcg ctggccgagc gtttttccaa gcggtttgga acgcccgtca gcgtggatag
    30301 caaaccgatg ccgcgcatga ccaagacgat ggaagctgct atgtaccgcg tggcgcaaga
    30361 agcacttaat aatgttgccc gccacgccaa ggccacacgt gtttacattc gcctttggat
    30421 gtctcccgga gagcttcact gccgcattcg cgatgacggc gtcggtttcg atcccgccgc
    30481 tttgccgagg gctagcaaaa acgccctggg catcatcggc atccgggagc gcctcaatgt
    30541 tctagggggt tcactggaaa tacagtctgc accacaacaa gggaccgagc ttaaaatcga
    30601 agttcccgtg gaggattaag ccatgccgat acaagttctg cttgccgacg atcaccaaat
    30661 cgtccgccag tcattgaagg ttttacttga aaaagaaggg ttgaaaatcg tcggcgaagc
    30721 ttcgaatggc caggaggccg tgcgaattgc cgaatccatg cacccggatg tcgccgtgct
    30781 cgacgtgagc atggccgtcc tcaatggcat tgatgcggcc aaggagattc aaaaagtctc
    30841 cccgcagacg aaaaccatct ttcttaccgt ccacgacgaa gatccctact tgctcgacgc
    30901 tcttcgcgtt ggtgcaaagg gttatgtcgt gaagactcac gccgccgaaa acctcgttca
    30961 ggccatacgc gaagcttcgc gcggcggtgt ctatctcagc ccggaagtat cgcgcgccgt
    31021 ggttcaggcg taccagaaca aaacagagct ctcctctgag cccttgagcc cccgcgaacg
    31081 ccaggtcctg cagctcatcg ctgagggaaa aacaactaag gaagtagccg gcgttctcaa
    31141 tatcagcgtc aaaaccgctg aaacccatcg cacgcgcatt atggagaagc ttgacattca
    31201 tgaaacagcg ggcttggtcc gctacgccat ccgaagaggt ttggttcagg cgtagaaaat
    31261 agcgacgaca caacccctac gaagtactaa ggaattatcc cgcgcggaat tcagccatgc
    31321 acctatggcg gacttctgat tgacttccta agattgggtt gttcagcgac ttttggtcgc
    31381 actgggggtt aaatccctcg tttctatctg aaacatcttt ttgatttttg aaaacagctt
    31441 ggtcccttgg agttttatga acgcttcggc tcgtgttatg gaactggatg gctcggtcgg
    31501 cacgcgtgaa ctgcgcggtg aattgaattc accgtcatca ttagaccata ttcccgcact
    31561 tgaagagctc tctgctaccc gaagtccggc aatggccgaa ctacgccacc agatcgaccg
    31621 cgttgccaca gtagatgtgc ctgtgctgct gctcggcgag tcgggagccg gcaaagagtt
    31681 cgctgctcgc gcaattcatg ctcggtctct ccggaaccag cgcacattcc tgaaaatcaa
    31741 ttgcgcggct ttgccttctg aactcctgga gagcgagctg ttcggatacg aatctggcgc
    31801 gtttacaggc gcggtcaggc cgaagcccgg gctcttcgaa ctgtgcgacc acggcacgat
    31861 tcttctcgat gaaatcggcg aaattcctcc cagtttacaa gcgaagctct tgcatgtgct
    31921 ccaagaccag cagttttcga ggctgggcgg ccgccgaatc attaccgtgg atgtgcgcgt
    31981 catcgccgca accaatatcg atatcaataa ggctcttgag caaaaaactt tccgcaccga
    32041 tctttattac cggctgaata ccatcactct tcacgtcccg ccacttcgcg aacgccgcga
    32101 agacattcct cttttcgtcg accacttcct ggactcgctt tctgttcaac tgggctgtgt
    32161 tccgcgcccg ctctcgccgc gcgccatgca ggcatgtttc gaatactctt ggcctggaaa
    32221 tgtccgtgaa ttaaaaagct ttatttgccg ccatctggtt ctccaggatg acgatgctct
    32281 cattgccgag cttctctccg cctctaatcc ggcggtatct ccgcaagagg gttcaactca
    32341 gacatcgggg ctgaagccgc gcatagcttc catccgcgct gaggcagaaa aacaactcat
    32401 cgaagagtgc ctgcgcaaaa gccgctggaa ccgcaccgaa accgcacgcg ctctccacat
    32461 cagcaccaaa actttgtgga acaaaatgcg gcaatacgga attaccgatt cttctcggcg
    32521 aggtgatatt cccaatggcg agcgcaaacg gaaattttca accgtggatt cgcaagcagg
    32581 cggcgacgtt gaatcgcact ttttcggaag catggacaaa gttcgcccaa ttgaattcgc
    32641 aaagcgcgcg ccagtcagtg caagcactgg gacgcgttag ccgctttttc ctcgcctcat
    32701 ggaacgggaa atactcgccc gcagctggtg tgtccgcgcc ccacgcccaa ttgaacgctg
    32761 ctcccattga aacacctgcg ccgccgagcg ctgaagtgaa acccatgccg tcaacggatg
    32821 ctccgcgtcc atttgctctt atctctccac cagcgcgtct ctcgcttgtc cccgaattcg
    32881 ccgaaagcac gccgaaattc tccgtcgcgt caactgaatc tcctgaaccg aaccgtttct
    32941 cggccaacac gccatcagcg gcttgcgaat cagccgctga acacgcccgg gccattgccg
    33001 aggcgcgaca gactctcgca aatcagcaag cggaattcgc acggcggctc gcaaagcagc
    33061 tcgactcatt taaccgtcag atcgcctcgc aactggagaa tctgtccggc caggtgattc
    33121 agcgctttgc tgaggaactc aatgcccatg ccggccacgc cctgaacgcg ctgatgtccg
    33181 actgggcgga gcagaatcgt gcgctggtcg acactgaatg caaccgccat ttggaccagt
    33241 tctccgctcg ccttcagtct ctttccaccg cgcacctgga aatctaccgg aaggagatgc
    33301 agaacctgtc gtccaacctg aagagccgtc tgcggagtgt ggctcatgct ctgcaggatg
    33361 tagggcccac ctcacaccgc agctaggata gataggagaa tgggataaat cgctccttag
    33421 cctgcgtgta ttgcaggacg ttattacctc gcgcgcgcgt ttccggtgac atacctcccg
    33481 atgccgagtc cgctgaaaca gaagtgcaag cgcggaaaac cggcaaaaaa ttatcgatta
    33541 gaaatttggc gagcctttgt tttcaatgac ttacggtcaa ttctaatttt tggtcgcgaa
    33601 ggagttcagc tccaagattg tggccggcag ggggaaggtc attgctcagt tctgaggaat
    33661 gcggatgtcg ttgttcagtc ttcgggagga cgccgatgga ggttcgcaag gagaagctcg
    33721 cggggaactg gtaaagggag tgagttccga ggatccctac ggtggaaggg agtcctaccc
    33781 gattgatctc ctttatgcgc tacgcgaacc ccccatcgtg tttggtgagc catatttaac
    33841 ctgagttaga gctctccgca ataccgtaat ccctgcatgc tcaccaggta aaatccccta
    33901 ttccaaaggc ttgagacgga aacgtcgccg gatatggcga tctagaggaa gcacggcgca
    33961 tcgcgaaaga gttataagga aaatactcga cgctggcgag ggattccaac gcagccccta
    34021 cttaactcca tgtattgttg agatttgtac cttgcgccta tgcttcgagc acactcagcg
    34081 agaggagttg ttcggcccgg ctggtgcatt tagacccgct ccgactagtt atggcgcatg
    34141 gtgagaaatt cgggcagaag aacgcgatag ttcaaccgtc gcgtgacggt acagtttcaa
    34201 gttctcaagg gggcgagcgc cgcgctacga acgttcgttc tattaccagc gcgccggcgc
    34261 gggtgcgcag ttgcgacctg cccgccgcaa tcctgcactc cgccgaggac gcgattatcg
    34321 ggctggccct cgatggcacc atcgtgagct ggaatccggg cgccgagcgc ctgttccgat
    34381 tcactccaca cgaagccatc agccagccgt tttcgctgtt tctggcggcg gaggcttcca
    34441 cggaacttct tcacctgctg gccgaactgc cgtccggaag gcgtttctcg cctcgcgagc
    34501 ttgaatgcac gcgcaaagac gcggggccga ttttttgctc gctttccgtc tcaccaatcg
    34561 tttctccgtc gggagaaatc gttggcgcat cgctcatcgc gcgggaagct ggctcgttcc
    34621 accagtctga gcacgaactc cggcacgcga acaagaagct ctctgagtcg gtccgcaacc
    34681 tggagcaacg caaccgcgaa atgaccattg tccagcaact cagcacgctg ctgcagacct
    34741 gcctttcagc agcggaagcg cacgacgttc tcgggcagtc tgtgccaaaa ctttttcctt
    34801 cctgctccgg cgcgcttttt gagtggaata attcggtgaa actgctcgag gcgacggtca
    34861 aatggggcga ggcatcggtg ggagaggcgg tattcaatcc ggagcagtgc tgggcgctgc
    34921 ggcgcgggca ggtgcatcgc gtggcggatt caagcgcgcc cgtgctatgc gcccatctct
    34981 cgcggctctc ctcgacgaat tccatctgcg cgccgctgct cgcatccggc gagacgctgg
    35041 gcgttctgag cgtgcagggc agcccctacg agctaagttt gcctaaagac gtgcagcagc
    35101 ggcttctctc ggctcgccag caattggtgg agatgcttgc aggacacgtg gcgctcgcgc
    35161 tggccaatct caaattgcgc gagagcctgc gcgcacagtc tattcgcgac ccggtgagcg
    35221 gacttttcaa ccggcggtat ctggatgaaa cgctgccgcg cgaaatccac cgcgccagcc
    35281 gcgatgggcg cgacctcggc atcatcatct gcgacctgga caatttcaaa tcgttcaacg
    35341 acacgtacgg gcacgccgcg ggcgacacgt tgctgaaatc gttcggcgac ctggtgggga
    35401 aagtggtgcg cgcgggcgat attgcgtgcc gctacggcgg agatgaattc gtgctgatcc
    35461 tgctcgacac ttctctcgaa acggcaaagc gccgcgccga gctgctcgaa cgcgagttca
    35521 gccgtacggt aattcagtac ggcgatatgt tcctgaatcc tggagcgcta tcgctgggcg
    35581 tgagcgccta tcctgtgcat ggcgcaaatg ccggcgcgct gctgcgcgtg gctgatgagg
    35641 cgctgtatcg cgccaaagcg gcggggggca aacgcgtgat tcttggtcaa ctcgtcgatt
    35701 gaaagcttcc cactcatttt tgcgggacgg actttcgcgt tgcccggctc atccccaaat
    35761 tctctgagcg ccttgcgctg acgcaaggca tcgcaggact ggttgagtcg aacttttagt
    35821 gcctccagac gctcgtaatc ctttgcggaa atttcgtcct ggctatataa gccttctttc
    35881 tcctgaccta aaacattgat gtggctctgc agcgattgaa tttcttgtca cgcttcggac
    35941 gggccaaaga attctcctcc tgttgagtca tccataagga aaaagatgcg gaatcgatga
    36001 agagagtcgt gcatggcagt ttttccggtg cgatgactca agcgatgttt cacccattga
    36061 tagcgttccg ggcgctccat aaagggtaag gcaatcctgc gaggcgatta atcactagcg
    36121 cctgtgctac cagcaataat acggccactt cgataatcac caactcgcgt ggtttcgaga
    36181 tgattccgag ggaaacaatc aaggtagtgg cgccggccgg cggatggctc acgtcgagca
    36241 aaaccataaa cgcacccgtg gctgaaagcg aaagagccga ggccacaatt ctcggccaaa
    36301 aaattcccgg atgcactccg aaaggcagtg ctcccgcgcc ggtgactaca aatgctccat
    36361 aaccgcaaag gagcccgatc gcatgtccaa tgattgtgtt ccgggggctc gacgtttttc
    36421 cgagcggaga aaaaaacagc agatatgcgg tcggccccag cgagggaaac acaaacgggt
    36481 tgcggctaaa gaacgccaac aaagccagga gcgcaatcgt aacgaagcca ttcacaaaaa
    36541 cataagttga acgcaccagc cgcggcggga aatgcgacaa cagccaatca aggcgaagct
    36601 ttcgaaaaaa agaggaagcg tggtcagagt ccgcatggcc ctgaccgtga gaatcggggc
    36661 tttgcatcag atgttaggca gcaggtcgaa gtactcgtca tcttcgtttt tgccgccttc
    36721 ccctttgcca agcaattttt ccgattcgat gaattcgatg cgctcaatcc acttgaccat
    36781 cttatagcca agctggtttt ccacgcgcag gcgcaaggga gctccataaa gttgcggaag
    36841 gagttgcccg ttcatgcggc acgccagaac gcattgcggc ttcagcacat tttcgaggct
    36901 ctgcgtgtcg tagtaaggtc cgccgtaaag cgcttcgccg aacgagaaaa acgctacgac
    36961 cttggcttgc ggttttggct gcaccagttc aatgagcttc ttcataggga tgccgcccca
    37021 cttcgcgatg ccgctccagc cctgtatgca atgatgcatg gtgatgtgtt cgacttcacc
    37081 gagccgctcg atgtcagcca gagaaagctc aaccggatga ttgaccagcc cgcccacttt
    37141 gagccggaaa tcggcaaatc ccgtttctgc catgcgcttc cagtcttcac gaatgggcat
    37201 ttttccgttg ggccagaaat aaggagatat ttgctgctgt gtgtacttct gctgcgggag
    37261 caagcggttc aaggtaatca gttgcatcgg gtacgtcacc gacttgagcg catgttgcac
    37321 gccgcgagga tgattccacg aaatatagtg cgccgcgacc cacgagagaa ccaccacgcc
    37381 aatcccgagg aatccaagat acatcccaat tggattctgg tcgtcagtgc ccatcacgat
    37441 gtggttcata ttgcgcgcga agccggtcat cacaatcagc gtgacgtgta cgaccaaaaa
    37501 tgccaggaag ctgaacattg tcaggaagtg tatggagcgt gctgactggc gcccgccgaa
    37561 tatccgcgcg taccacggaa agcggttcac cacggccggc gacatagcaa ttccagtggc
    37621 aatcgcgagc gggccaaaaa cgaacaccac cgtgaaatac gcaatctgct gcagcgcgtt
    37681 gtagccatag aaaccgttcg gctcagaagg caggtgaaaa gtcgcgtagt gaacgaaagt
    37741 attccacgcc tgaagaacaa ccaccggaga agccggcaca atacgctgcc attgcgcggt
    37801 ctcgaaaagc atcacgatga aaatgattcc cgtcgctata aatccgtgta cgtcgataaa
    37861 atgccatact cgcgcaagtc caatggtatg gcggtacccc ggtgttccaa tgagcggcga
    37921 tatgtatcgc gcatcgtctt tcgcggtcca gatgcgatct ttcggaacct tcagcggagt
    37981 aaaccgaatc cattcgctcc cgggcgtgca gccgtcattg aaataaagcc gaggatggtc
    38041 cactaagatc gagaggccgc tgcgaatgag catcattacg aaaagaaaat tgaagaaatg
    38101 gcagtagcgc acccagaccg gaaatccgtg cgggcctgca aaattattcg gataaatctg
    38161 cgggactgca gggacgccgg gcattccggc cacggcgact tggatccagg cagcagcgac
    38221 taacgcggcg atcgcgatca cgattccgat cagaatcgaa ggacgaatcc aaatgcgaaa
    38281 gcggcgatcc ggcggatagt gaacagcttg gtgggcagca tcgaatcgaa ccattgctat
    38341 cctcgacgaa ctgacgttgg ctttagcggc agcgcagaac ttcgctttta tgcggaattc
    38401 tgcgccttgc ccttctccag gccgcggcct ttgtaagagc agcttgccga actacttgct
    38461 cgattatgcc tgtgtagcca ctgataccct caaattttta tcctcgttag cgagcaaatg
    38521 cgtattacac gcgccacatt acagacgcct tgaaattcta ctgaagctag aaccaatttt
    38581 gttccattgg cgctgtgact ctcagccttt tttcgcaggt caccatccaa tctcgctccc
    38641 attacactct gaaggttcca ggcacaataa ttcggccaag gatgtttcct ctgcgaaaat
    38701 gatttagact agcactatta aaaggatggg cgtgtagctc agctgggaga gcacctgctt
    38761 tgcaagcagg gggtcgccgg ttcgatcccg gccacgtcca ccaaattctt cctgattgca
    38821 aaagacttaa cgaaaacgtt ctgcctcaac agcgcgatta ttcgtgcaca ctgtgcacga
    38881 atgcgtcaca attctagcct atcaaagcta tgcaagctgg gcttggacgc tcttgcctcc
    38941 ttccgcgatg ctcgaacttg caggcattac tgcgttcgct ctaaatatct tcgggacctt
    39001 cttgctgcag ccaagtcgcg cgcagcaccg ccaattgatt attggcattc cgaaagcca
//
LOCUS       DATNDT010000377        10005 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2071695, whole genome shotgun sequence.
ACCESSION   DATNDT010000377 DATNDT010000000
VERSION     DATNDT010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10005
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2071695"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            525..1304
                     /locus_tag="VN788_12310"
     CDS             525..1304
                     /locus_tag="VN788_12310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21355.1"
                     /translation="MKAAITTAEGAQGENSQRVRVAAGEYEVLQGAHAGGIGSYAPGV
                     YNFRESWTLWRLADGSFDVEGEREYESPKDELHSNRFSVHLSPDFRTLSVKEFRKLRW
                     KPDSGPLSCEFQPTEMMCTSSAKDPSQSVNLNVPMKDAYGFLWPISAFSLSNITRRAA
                     KIHGRLTPVQLVTVEEPSPRNPVFVSTLDGNLKYLAEEEITLAGKKWRADKFELKVPL
                     HPAFIIWTSPEGLLLRFAPEGKSTKTAEGLQLVHFQQEIAF"
     gene            1506..2390
                     /locus_tag="VN788_12315"
     CDS             1506..2390
                     /locus_tag="VN788_12315"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HXU21356.1"
                     /translation="MKILLAIDSSSVSQNVIAEAATRPWPSRTRFCVLHIVDVLGMGR
                     SLAMIDEEKRSGQALVAAAAEKLIRAGRETSTDILVGFPRKSVVQYAQEWGADFVMVG
                     SHGQGALLRFLLGSVAQAVLRTAPCSVEVVRRRIDESSSSRGIKILLGVDGSEGASAA
                     AKSVASRPWPAGSQVKIISAVQLLVPGNELMMSSSAIYPTSLLEEVWGEVRSSAREAV
                     AETRKMLEAAGMKIPAGETTPEGDPRVVLLAQAKEWDADLIVLGSYGRRGMDRILMGS
                     VSESVALHAHCSVEVIRG"
     gene            2494..4338
                     /locus_tag="VN788_12320"
     CDS             2494..4338
                     /locus_tag="VN788_12320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXU21357.1"
                     /translation="MRALFARWTGAPAISVLTIGAILLHLILRYALVTTRTIYLAPLI
                     AALIFGGIPLVVTLTKKAFAREFGSDLLAGISIVTAVLLGEYLVGAIVVLMLSGGTAF
                     EEFASRRASSVLDALAKRIPQIAHRKAGASLSDVPLDAVAVEDVLVVFPHEICPVDGA
                     VLEGHGKMDEAYLTGEPFEISKTPGSLVLSGAINGDSALTIRAEKLAVDSRYATIMRV
                     MQETEQKRPRLRRLGDKLGAWYTPIALLLAIATWAVTQESHRFLAVLVVATPCPLLIA
                     IPVAVIGAISLSARRGIIIKNPGVLEEIDLCRTLIFDKTGTLTYGKPKLSEIECADGY
                     QRKDVLRLAASLEVYSKHPLAMAILDAARAEGLRTEAASEISERPGQGLRGTVGSRQV
                     LITGRNKVAVQNEKLPPIAPGLECLVFLDGNFAAAFHFHDAPREDSHLFLEHLKPRHA
                     ITKVMLVSGDRESEVRYLAGIVGIKEVHSAKSPEEKVEIVREAARRQKTLFVGDGIND
                     APAMQAATVGVAFGLSSDITSEAADAVVMETSLGKVDELMHIGRRMRHIALQSAVGGM
                     ALSVVGMIVAALGYLPPVGGAIAQEVIDLLAVLNAVRVALPSKVLTDF"
     gene            4523..5011
                     /locus_tag="VN788_12325"
     CDS             4523..5011
                     /locus_tag="VN788_12325"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HXU21358.1"
                     /translation="MTDKPPTAESGEARVTNMKIGDTTECSKAISDAVAKRAYEIYER
                     SGRQPGCDRKNWFLAESEILKPLSCGLLKSKDGVTVSICRSSLNAKDIEVCVEPHRLV
                     VMAHKDSNSTIGPAATYRVLSLADEVEPSSVKLKAHGRLLEIKLSKAGAIKKTLLAKV
                     AA"
     gene            complement(5026..6237)
                     /locus_tag="VN788_12330"
     CDS             complement(5026..6237)
                     /locus_tag="VN788_12330"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006195145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HXU21359.1"
                     /translation="MKILVLNSGSSSQKMSLYHIANALPQSPPAPTWEGKVEWNGDQA
                     EVRVQTSQGKKLKEQTTIESRQQGIGLLLDMLCSGKTHAVSKSSEIDVVGHRIVNGGQ
                     EYEKPTVITSDVKAAIAKMSAFAPLHNRVEMEGIDIIEKRFGAVPQAAVFDTGFHARL
                     PESAFVYPGPYEWFARGIRRYGFHGINHQYCAERTAQLLGKKLNALKMVTCHLGNGCS
                     LAAIRNGHSVDTTMGFTPLEGLMMGTRSGSVDPSILTYLMREGKSTAEDLDQLLNTKS
                     GLLGISGISSDMRQIVSAMKKGHRRAKLAFRIFVHRLVSYIGAMTAVLDGIDALVFTA
                     GIGENSPEVRAAACANLGYLGLRLDSQKNRRLAADHQISTRNSSVRVLIIRAQEDWAI
                     ARDCWKLASHR"
     gene            complement(6241..8634)
                     /locus_tag="VN788_12335"
     CDS             complement(6241..8634)
                     /locus_tag="VN788_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015079103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HXU21360.1"
                     /translation="MASAGSAIPSESKRPLSSEELRKMHAYWRAANYVSVGQIYLLKN
                     PLLREPLKLEHIKPRLLGHWGTTPGLNFIYVHFNRLIKKHDLNVIYIAGPGHGGPGLV
                     GNTFLEGTYSEFYPHITQTEDGMRKLFKQFSFPGGIPSHVAPETPGSIHEGGELGYSI
                     AHAYGAAFDNPNLLICCVVGDGEAETGPLAASWHSNKFLNPARDGAVLPILHLNGYKI
                     ANPTVLGRMDDAQLTHLFTGYGYKPFFVEGREPQSMHQLMAHTLDTVIQEIHSIQRSA
                     RAGKSTGLPEWPMIILRTPKGWTGPKIVDGKPVEDTWRAHQVPITDFASHPGHLKMLE
                     DWMKSYKPEELFDEKGELVPELRELAPKGNRRMGANPHANGGLLLRDLVLPDFRDYAV
                     DVAKPGTKFVEGTRVLGKFLRDIMKLNAGSKNFRVWGPDETASNRLDALYEATDKVFM
                     EPILATDEHLSANGRVMEVLSEHMCQGWLEGYLLTGRHGFFSCYEAFIHIVDSMFNQH
                     AKWLKVTRKIPWRRPIASLNYLLTSHVWRQDHNGFTHQDPGFIDHVINKKADIVRVYL
                     PPDANTLLSVADHCLRSRNYVNVIVAGKQPALQWLDMEKAIEHCTTGIGIWEWACTDQ
                     GAEPDVVMACAGDIPTLETLAAVDLLRQHFPDIKIRVVNVVDLMTIQSPTEHPHGLSD
                     RDFDSMFTTDKPIIFAYHGYPWLIHRLTYRRTNHDNLHVRGYKEEGTTTTPFDMTVLN
                     DLDRFHLAGDVVDRVPRLERIGAHFKHFLQEKLVQHKEYICEHGDDLPEIRDWKWPY"
     gene            8924..9640
                     /locus_tag="VN788_12340"
     CDS             8924..9640
                     /locus_tag="VN788_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU21361.1"
                     /translation="MRAPYGLEIIENCLACPHLQERVFCNLPPAALQRLSAITSASSY
                     PKGATLFVEGQTARGVFILCNGRVKLSTSSIDGKTLIVRIADPGEVLGLPATVTGKPY
                     ELTADVVEPAQANFISRTDFLDFLRDHGEAALRVAQQLGETYHAAIAEMRSIGLAHSA
                     GEKLARFLLDWCASHGNGKEETSAKLTLTHEEIAQMIGASRETVTRLFTDFKKRQLLQ
                     VKGSTLTLSNKEALEHLAGS"
BASE COUNT         2217 a         2666 c         2842 g         2280 t
ORIGIN      
        1 ggttgaaacc ggcggtcttg aaaaccgtta gacctcaaaa gggtctcgtg ggttcgaatc
       61 ccaccccctc cgccagtcag tcaccggttt tctccgtgtt cccctagttg cgagagatac
      121 ctcttatttg cggcccaatt gccgcgaatt tgccgcacgc ccttgcgctt tctctggttc
      181 ttgtgcaaaa ggcaaaacga atcgggccaa tattctctga ggttaagcat gaggtgcact
      241 ttcgaaactg acgaacgcgc tcggagtggc gcaagtaatc gatcaaggga tcagaacgtg
      301 ttaaagcccg acgactacgt caaggtcgaa ttcgtcaagg aaagcaccgg cgaatcggaa
      361 tgaatgtggg tagaagtctc ctctgacgat aggtcaagtg tgatttcgga gtacacttaa
      421 cgtacataat gcgattcaag gatatattcg ccgtctgtgc acttctgttt ctgcttgtgg
      481 caatgcgccg tcccaattca aatttcaacg gcagtggtgc gcacatgaag gccgcaatca
      541 cgacggccga gggcgcgcag ggcgaaaact cgcagcgcgt tcgtgtcgcc gcaggggagt
      601 atgaagtcct tcagggagcc catgctggtg gaatcggctc atacgctccg ggcgtatata
      661 actttcgcga atcttggacg ctttggcgat tggcagatgg cagctttgac gtggaagggg
      721 aaagagaata cgaatcgcca aaggacgagc tgcacagcaa tcgattttcg gttcatcttt
      781 cgccggattt ccgcacgctg agtgtgaagg agtttagaaa gctgcgctgg aagccggatt
      841 ccgggccact cagttgcgaa ttccaaccta cagaaatgat gtgcacttcc agtgcaaaag
      901 atccaagcca atctgtcaac ctaaacgtac cgatgaaaga cgcgtatggt ttcctctggc
      961 cgatttccgc cttttcgttg agcaatatca cgcgtcgcgc cgcgaaaatc cacggcagat
     1021 taactcccgt tcaactggta acagtcgaag aaccgagtcc acgaaacccg gtgtttgtgt
     1081 ccacgctgga tgggaatctg aaatacctcg cagaagagga gatcacgctg gcgggtaaga
     1141 aatggcgtgc ggacaaattc gaattgaaag tcccgctgca tcccgcgttc atcatctgga
     1201 cgtcgcccga aggtttactg ctgcggtttg ctcctgaggg caagagcacc aaaacggcag
     1261 agggtttgca gttagttcac ttccagcaag aaatagcttt ctgaagtcgg gaacctcttc
     1321 cgttcaacac tgattctgca ctctatttca gaaatcaaac tatgtttacg cgtcacgagc
     1381 caatgtgacg ttggtcacgg tgagcgaatc tccgccttgc gaaactgtgt ctataagggt
     1441 tgcggatatg gaattcttgc tcgtgcgtca gacgagtttc aaatgaacgt gatgagaggc
     1501 gcaaaatgaa aattctgctg gcaatcgatt cgtcgagcgt gtctcaaaac gtaatcgccg
     1561 aggcggcgac ccgcccctgg ccgtcgagaa cgcggttttg cgttctccat atcgtggatg
     1621 ttttgggcat ggggcggtcg ttggcgatga tcgacgaaga aaaacgctcc gggcaagctc
     1681 tcgtcgcagc agcagctgag aaactgattc gtgccgggcg cgaaacgagc actgacattt
     1741 tggtcggctt cccgcgaaag tctgtcgtgc aatacgccca ggaatgggga gctgactttg
     1801 tcatggtcgg ctcgcatggt cagggcgcgc tcttgcgatt tctgctgggc agcgttgcgc
     1861 aagcggtgct ccgaactgcc ccgtgttcgg ttgaagttgt ccgccgaagg attgacgaat
     1921 cttcctcaag tcgggggatc aagatactgc ttggcgtgga cgggtctgag ggcgcctcgg
     1981 ccgccgcgaa atcagtggcg agtcgtccgt ggcctgcggg cagccaggtc aagatcatca
     2041 gtgcggttca attgctcgta ccgggaaatg agttgatgat gtcgtcgtcc gctatttatc
     2101 cgacgagcct ccttgaagag gtgtggggtg aagttcgcag cagtgcgcgc gaagcggtcg
     2161 ccgagacgcg aaagatgctg gaagccgcag ggatgaaaat ccccgcggga gaaacaacgc
     2221 cagaaggaga cccccgagtt gtcttgcttg ctcaagcgaa ggaatgggac gcggacttaa
     2281 ttgttcttgg ctcgtatggg cgtcgcggaa tggaccgtat actcatggga agtgtctcgg
     2341 aatcagtggc actgcacgcc cattgctcag tggaggtcat tagagggtaa tgtacacagg
     2401 agaactcatc gaaccgtgag ttcggtctcc aaggccgaga gagaatttgc gctaaagaga
     2461 gataggcgcg cggctcccgg tccgcggcgt gccatgagag cgctgtttgc tcgctggacc
     2521 ggagctccgg cgatttccgt tctgaccatc ggtgctattc ttcttcattt gattctgcgc
     2581 tatgctctgg tgaccactcg gacaatttat cttgccccac tcattgccgc cctgatcttt
     2641 gggggcattc cacttgttgt cacgctgacg aagaaagcgt tcgcacggga atttggctcg
     2701 gatttgctcg cgggaatctc gatcgttacc gcggtcctgc ttggagaata cctggtaggc
     2761 gcgatcgtcg tgctgatgct ttcgggaggc acggcgttcg aggagtttgc atctcggcgc
     2821 gcatcgtcgg tgctcgacgc gttggcgaaa cgtattcctc agatcgcgca ccgcaaagcg
     2881 ggtgcgagtc tctctgacgt gccgctcgac gctgttgcgg tcgaggatgt cttagttgtg
     2941 ttcccacatg aaatttgccc cgtagacggc gccgtcctcg aaggccatgg aaagatggat
     3001 gaggcgtatc tgacgggcga gccattcgaa atctcgaaaa ctcctggttc gctagtgctc
     3061 tctggtgcaa tcaatggcga ctcggctttg acgattcgag ccgaaaaatt ggcggtggat
     3121 tcccgatacg cgaccatcat gcgcgtaatg caggaaacag agcagaagcg gccacgactc
     3181 aggcgcctgg gcgacaaact gggcgcgtgg tacacgccga ttgcgctgct tctcgccatc
     3241 gccacgtggg cggtgacgca ggagtcccat cgctttctcg cagtattggt tgtcgcgacg
     3301 ccctgtccgt tgctgattgc cattcccgtt gcagtgattg gcgccatttc cctctccgcg
     3361 cgccgcggaa tcatcatcaa gaatcctggc gtgctggagg aaattgacct ttgccgaact
     3421 ctgattttcg ataagacggg aacgctcaca tacggaaaac cgaaactgtc tgaaatagag
     3481 tgcgcggatg gttatcaacg caaggatgtc cttcggctag cggccagcct ggaggtttat
     3541 tcgaagcacc cgctcgccat ggccattctt gatgccgccc gagcagaggg gctgcgaacg
     3601 gaagcagctt cggaaatcag cgagcgcccg ggccagggat tgcgggggac ggtgggaagt
     3661 cgacaagttc tgatcaccgg gaggaacaaa gtcgcggtgc agaacgaaaa actgcccccc
     3721 attgctcctg gactggaatg cctggtgttt ctcgatggca atttcgcggc ggcgtttcac
     3781 ttccacgacg cgccgcgcga ggacagccac cttttcctgg aacatttgaa gccgcgccac
     3841 gcgatcacca aggtgatgct cgtctccgga gaccgggaat cggaagtccg ttatctggcc
     3901 gggatagtcg gaatcaagga agtgcattcg gcgaagagtc cggaagaaaa agtcgaaatt
     3961 gtgcgcgaag cggcgaggcg gcagaaaact ctttttgttg gcgacggaat caatgatgcg
     4021 ccggcgatgc aggcagcgac cgtgggtgtg gccttcggtt taagcagcga catcacttca
     4081 gaagcggccg acgcggtcgt gatggagact tcgctgggga aggtagatga actgatgcat
     4141 attggccggc ggatgcggca tatcgcattg cagagcgcgg ttggcggcat ggcgctcagc
     4201 gtagtgggta tgatagtggc ggccttaggt tatctcccgc cggtcggcgg agcgatcgcg
     4261 caggaagtga ttgatctcct tgccgtcttg aacgccgtgc gcgttgcctt gccgtccaag
     4321 gtcctgaccg atttctaaga atgcacaacg cttacattct gaatctctag cttgcatctt
     4381 cgtcataacc gcctgtaact aatcgttttg tgtcccgtga cgcacgtcac agtcaccagt
     4441 gacgcagatc atcacgctcc agtcagccct ggtgctacaa atggtttgaa cgatgctgtt
     4501 ggcggaataa aggggaaaga atatgacgga caagccacca actgcggaat ccggcgaagc
     4561 gcgggtcacg aatatgaaaa ttggggatac gacggagtgc tccaaggcaa tttcggacgc
     4621 agtagcaaag cgcgcgtacg aaatttatga acgctcgggg cgccagccag gctgtgatcg
     4681 caaaaactgg tttctggccg agagtgaaat tctcaagcct cttagctgtg ggcttttgaa
     4741 atcgaaagat ggcgtcaccg tctcgatatg ccgttcctcg cttaatgcga aagacattga
     4801 ggtatgcgtc gaaccgcatc gtttggtcgt gatggcgcac aaagattcga attcgacgat
     4861 tggaccagcg gcaacttatc gcgtgctttc cttggcggac gaagtcgagc catcctccgt
     4921 gaagctaaaa gcgcatggcc gtctgctcga gatcaagttg agtaaagcag gggcaatcaa
     4981 aaagactctt cttgccaagg ttgccgcttg atttagcgtt tgcggtcagc gatgagaagc
     5041 aagcttccag caatccctcg caatcgccca atcttcctgc gcgcgaatga tcaggacgcg
     5101 gacggaggaa ttgcgagtgg agatttggtg atcggccgcg agccgccgat tcttttgcga
     5161 atcgagtcta agaccaagat atccgagatt ggcgcaagct gccgcccgca cttctggcga
     5221 attttcgcca attccagcag tgaaaaccaa agcgtctatt ccgtccagga ccgcagtcat
     5281 tgcgccaatg taggaaacga ggcgatggac aaatatccgg aaggcaagct tggcgcgccg
     5341 gtggcctttc ttcatggcgg acacgatttg ccgcatgtca ctggagatcc cagagattcc
     5401 caacaggccg gatttcgtgt tcagcaattg atccaagtct tccgctgtgg atttgccttc
     5461 acgcattagg taagtgagga tgcttgggtc aaccgagcca gagcgggtac ccatcatgag
     5521 tccttccagc ggagtgaagc ccattgttgt gtccacgctg tggccatttc ggatcgcagc
     5581 cagagagcaa ccgttcccaa ggtggcaagt caccattttg agcgcgttta gttttttgcc
     5641 gagcagttga gcggtgcgct cagcgcaata ctgatgattg attccatgga agccatatcg
     5701 gcgaatgccg cgcgcgaacc attcgtaagg tcccggatac acaaacgcgg attcgggcaa
     5761 ccgagcatga aagccggtgt cgaacacagc cgcttgtgga actgccccaa atcgcttctc
     5821 gatgatgtcg attccttcca tctcgacgcg attatgcagc ggggcaaatg cagacatctt
     5881 cgctatggcg gcctttacgt cagatgtgat gaccgtcggc ttctcgtatt cctggcctcc
     5941 gttcacgatg cggtgaccta cgacatcaat ctcggaagat ttggaaacgg cgtgcgtctt
     6001 gccagagcac agcatgtcga gcagcagccc aatcccttgt tggcgcgatt caatcgtggt
     6061 ctgttctttg agctttttac cctgggatgt ttgaactcgc acctctgctt gatcgccgtt
     6121 ccattcgact ttcccttccc aggtgggcgc gggcggagat tgcggcagcg cgtttgcgat
     6181 gtgatagagg gacattttct ggctgctcga accggaattg agcaccagta ttttcattgc
     6241 ttagtagggc cacttccaat cgcggatttc cggaaggtcg tcgccgtgct cgcagatgta
     6301 ttccttgtgc tgaaccaact tctcctgaag gaaatgcttg aaatgcgcgc caatgcgttc
     6361 taagcgagga acgcggtcaa cgacgtcgcc tgcaaggtga aatcgatcca aatcgttgag
     6421 aacagtcata tcgaaaggag tggttgttgt tccttcctct ttgtatccgc gaacgtgaag
     6481 attgtcgtgg ttcgtgcgcc ggtaagtcag gcgatggatc agccacggat agccgtgata
     6541 ggcgaatatg attggtttgt ccgttgtgaa catcgagtcg aagtcgcggt cggaaaggcc
     6601 gtgtggatgt tccgttggtg attggatcgt catcaagtcc accacattga cgacgcggat
     6661 tttgatgtct gggaagtgtt ggcgaagcaa atcgaccgcc gcgagcgtct ccagagttgg
     6721 aatgtccccg gcacaggcca tgacgacatc cggctccgcc ccttggtccg tacacgccca
     6781 ctcccaaatc ccgattccgg tcgtgcaatg ctcgatggct ttttccatgt caagccactg
     6841 caacgcaggt tgcttgccgg cgacaatcac attcacgtag ttgcgactgc gcaggcagtg
     6901 atcggcaaca gaaagcagcg tgttcgcgtc ggggggcaag tacacgcgga cgatgtcggc
     6961 cttcttgttg atcacgtgat cgatgaagcc gggatcttgg tgagtgaaac cgttatggtc
     7021 ctgccgccac acgtgcgacg tgagcaagta attcaacgag gcgatcggac gccgccacgg
     7081 aatctttcgt gttaccttca gccatttcgc atgctgattg aacatggaat ccacaatgtg
     7141 aatgaacgct tcatagcagg agaaaaagcc gtgccgcccc gtcaaaagat aaccttccag
     7201 ccagccctgg cacatatgtt cactgaggac ttccatcaca cggccatttg ctgaaaggtg
     7261 ctcgtcagtt gccagaatcg gttccatgaa gactttgtcg gttgcctcat ataatgcatc
     7321 caagcggttc gatgcagtct cgtcgggacc ccatacgcga aagtttttgg atccggcatt
     7381 cagtttcatt atgtcgcgca agaattttcc caggacgcga gtcccttcca cgaactttgt
     7441 cccaggtttc gcaacatcga ccgcgtaatc gcggaaatcc ggaagcacca aatctcttag
     7501 taacaatccg ccattggcat gcgggttcgc gcccattctt cggttgccct tcggcgcaag
     7561 ctccctcaat tctggaacca gctcgccttt ttcatcgaag agttcctcgg gtttgtagct
     7621 cttcatccaa tcttccagca ttttcaaatg gccaggatgc gatgcgaaat ctgtgatcgg
     7681 aacctgatgt gcccgccacg tatcttcgac cggtttccca tccacgattt tcgggcccgt
     7741 ccatcctttc ggcgttcgaa ggatgatcat cggccattca ggcaagcccg tagacttgcc
     7801 cgctcgcgcg ctgcgctgaa tcgagtggat ttcttgaatc accgtgtcga gcgtgtgcgc
     7861 catcagttgg tgcatgctct gcggttcgcg accttccaca aagaatggct tgtatccgta
     7921 ccccgtgaaa aggtgagtta actgcgcgtc atccatgcgg ccaagcaccg ttgggttggc
     7981 aatcttgtaa ccgttcaggt gcaggatggg aagaacagcg ccgtcgcgcg cgggattcag
     8041 gaacttgttc gaatgccaac tcgcagccag cgggcctgtt tccgcttcgc cgtcgccgac
     8101 cacgcagcaa atcagcaaat tcggattatc aaacgcggcg ccgtaggcgt gcgcaatcga
     8161 atagcccagt tcaccgccct catgaatcga gcctggcgtc tcgggggcca catggctcgg
     8221 aatgccgccc gggaatgaga attgcttgaa cagcttccgc atgccgtctt ctgtctgcgt
     8281 aatgtgcgga tagaactcgc tatatgtgcc ttctagaaat gtgtttccca ccaggcccgg
     8341 ccctccatgt ccaggtccgg cgatatagat gacgttcaga tcgtgcttct tgatgaggcg
     8401 attgaaatga acataaatga agttcaatcc gggtgtcgtg ccccaatggc ccagcaggcg
     8461 cggcttgata tgttcgagct tcagcggctc gcgcagaaga ggattcttca ggaggtagat
     8521 ctgcccgacc gatacataat tcgccgcccg ccaatacgca tgcatcttgc gcagttcttc
     8581 ggaggacagt ggtcgcttgg attcagaggg aattgccgat cctgccgaag ccatagagat
     8641 gatctcccat ttggagtcaa gcgcccgtgg ccggactacc gagcgattac aatcatatcg
     8701 caattacttt gcaccggcgg ggtcgccacc tattcgaact atccgcattt gccgatggcc
     8761 gtaacgtgac ctttatcacc cgcgtatgtg acccctgtca ctttcgaaag cgaaaattcg
     8821 agccacactc acactaaggg tcgacgaaat ggcagatcag agcaccagcg gcttcagaat
     8881 cgttccgcgt cgcaacgagt gtacaatagg gcgcggttcc tatatgcgcg ccccttatgg
     8941 cctcgagata atcgagaact gccttgcttg cccgcatcta caagagcggg ttttttgcaa
     9001 ccttccaccg gcggcgctgc aacgtctttc cgccatcacg tccgcttctt cttatccaaa
     9061 gggtgcaacg ctcttcgtgg aaggacaaac ggcgcgcgga gtcttcattc tgtgcaacgg
     9121 acgcgtgaag ctttcgactt cttcgatcga cgggaaaacg ctgatcgtca ggattgccga
     9181 tcccggagaa gtgcttggtt tgccggccac ggtcaccggc aaaccgtacg aactcacagc
     9241 agacgtggtt gagccggcgc aggcaaattt tatctcgcgg actgattttc tggattttct
     9301 tcgcgaccat ggggaagccg ctcttcgcgt ggcccagcaa ttgggcgaaa cctatcacgc
     9361 cgccatagcc gaaatgcgtt ccattggact cgcgcactcc gccggagaaa aactcgctcg
     9421 atttctcctc gattggtgcg ccagccatgg aaatggaaag gaggaaacca gcgcgaaatt
     9481 gacgctgact cacgaggaaa tcgcccaaat gatcggagcc tctcgcgaga cggtcactcg
     9541 cctttttact gacttcaaga agaggcaact attgcaggtt aaaggctcga cgctcaccct
     9601 gtctaacaaa gaggcgctcg agcacctggc cggaagttaa gctcctgccg cagaattcct
     9661 ccaacaactg agatccagtg ttcttggaaa cacgcaaggc ttgcaatgag atgttagcat
     9721 gtgagtcata caaaccgaat ctgttcgata ttgaccagac attagagccg ttcgactgta
     9781 gcctccacag gactgagcca gcccctccat tattgatgat gtttgagcgt cacaaccgtc
     9841 cgtgacccgg gtcacagacc tgacagacaa accggagcca aatatcgtcc gcagcatagt
     9901 gcagcggaga gatgctccaa catcaccccg agactttgtg gagtgcgttc ggggccccac
     9961 cgctgcagtg atgcgttgaa cgatccgaca ggaggatagt gatga
//
LOCUS       DATNDT010000378         3630 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_662113, whole genome shotgun sequence.
ACCESSION   DATNDT010000378 DATNDT010000000
VERSION     DATNDT010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3630
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662113"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="VN788_12345"
     CDS             <1..1634
                     /locus_tag="VN788_12345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU21362.1"
                     /translation="RESEELYRSTFENAPIGLAHISTEGKWLRVNRRLCSILGYSPEE
                     LLSLTFQNIIHPEDLAQSIAAAKDLLAGRSDRFSARRRYLRKDGAIAWVSLTASCVRD
                     SNHNVKHFISIFDDMSGRVDREASLGPAGVVGSSDGATTPGDLNRTEEKGFKIASTLE
                     ESEKRLRAAFSQTYSVLLLLRPDGTVVEANRAALEGMGFTRDQVIGRKCWEPWWSALP
                     NEVETLKASVARAAQGEVVREECSYCTSNGTVRFADRTLAPVRDDNGAVVLIVASGLD
                     ITEQKELLDKLEQRVHTRTQELEQKNAELRKLSARLLQIQDEERRRIARELHDSVGQL
                     LAAISMNASNISREKSSLSASAASAISENVKLVEQVSNEIRTLSHLLHPPLLDEIGLL
                     SALKWYIDGFSERSKIKVTADMPSDLGRLPRDVELSLFRIVQECLTNIHRHSGSPTAF
                     VRLARSRQGVTLEVKDEGRGFSANGESIVSGSDIPGVGLRGMRERVRQLGGQFEITST
                     GKGTLVSAKLPLAEDFSSTAHGTADFTPGQSQWKN"
     gene            complement(1657..3027)
                     /locus_tag="VN788_12350"
     CDS             complement(1657..3027)
                     /locus_tag="VN788_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mercuric reductase"
                     /protein_id="HXU21363.1"
                     /translation="MKYDAIIIGSGQAGNPLANALAERDWAVALIEKTHVGGTCINTG
                     CTPTKTMIASAQVAHYARNASRWGVKAGSVSVDLPAIVSRKNAIVERFRSGIERGIAK
                     HDKVRFYRGVAKFLSARTVQIDKDVIEGERIFINSGARTDPPKLNGLDAVGYLTNESI
                     MELQEVPDHLLVLGGGYIGLEFGQMFRRFGSRVTVIHRAEHLLPHEDSDVTTDLQKAL
                     EAEGIEFRLQSNPVEIERKQGQVRVTVTSGNQTSTIDGTHVLAATGRRPNTDDLSLDK
                     AGVEIDSRGYIKVNGRLETTAPGIWALGDVKGGPAFTHISYNDYQIVYANLIQSKNMT
                     TENRYVPYSLFTDPQLGRVGMTEKEARAAGRRLKMGKYSMSQVARAIERGETAGFMKI
                     IVDAQTDKILGAAILGAEGGELVQLLGAVMLAGAPYTILKGTVFIHPTLAEGFFGLME
                     SVKEVN"
     gene            complement(3153..>3630)
                     /locus_tag="VN788_12355"
     CDS             complement(3153..>3630)
                     /locus_tag="VN788_12355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU21364.1"
                     /translation="DAVIGSLGHLKKGHKDKRVLLVVTDGDDDASEKSFEDAVKAAEQ
                     SNATIYAVGVFSQDDLKTDKKMVRHSKKVLAELSEATGGQAYFPDNLEDVTPVCEQIA
                     QEIRNQYTIGYYPTDTAKDGSFRSVALKVTPPGGRGKLLVRTRTGYYAPKASSSGD"
BASE COUNT          767 a         1040 c          964 g          859 t
ORIGIN      
        1 ttcgggaaag tgaggaactc taccgctcta cgtttgagaa tgctcctatt gggctcgcac
       61 acattagcac tgagggcaaa tggctgcgcg taaatcgccg cctttgcagc attttgggct
      121 attcgccgga ggagttactc agcctgactt ttcaaaatat cattcaccct gaagatttag
      181 cccagtcaat agccgcagcc aaggatttgc tggccggccg ctccgatcga ttctcggctc
      241 gcaggcgcta tcttcgcaaa gacggcgcaa tcgcctgggt gagtctaacc gcttcgtgcg
      301 tgcgcgattc gaaccacaac gtaaaacatt tcatttcgat atttgacgac atgagtggac
      361 gtgtggatcg cgaagctagt ttggggccag cgggtgtcgt cgggtcttcg gatggcgcaa
      421 ccacacctgg tgatctcaat cgcaccgagg aaaaagggtt caagatcgcc tccacgctgg
      481 aggaaagcga aaagcgcctg cgtgccgcgt tcagtcagac gtactccgtt ttgctattgc
      541 tgcgcccaga cggcaccgtt gtggaagcaa accgggcggc cttagagggc atgggcttta
      601 cccgggatca agtcatcgga cgcaagtgct gggaaccgtg gtggtccgct ctgcccaacg
      661 aagtggaaac ccttaaagcc agcgtcgcgc gtgctgcgca aggggaagtg gtccgcgaag
      721 agtgttccta ctgcacatcg aatggaacgg tgcgttttgc ggaccgcact ctggcgccgg
      781 tcagggacga caatggtgct gtagtcttga ttgttgcctc cggcctcgac atcaccgaac
      841 agaaagaact cctggacaaa ctggagcagc gggttcacac acgcacgcag gaactagaac
      901 agaagaacgc cgaactccgc aagctgtcag cgcgcctgct tcagattcag gatgaagaac
      961 gtcgccgcat cgcgcgcgaa ttgcatgata gcgtggggca gttgctcgcc gctattagca
     1021 tgaatgcatc gaacatttcc agggagaaaa gtagcctcag cgcttcggcc gcatccgcca
     1081 tatcggaaaa tgtaaagcta gtcgagcagg tatctaatga gatccgtact ctttctcact
     1141 tgttgcatcc acccctatta gacgaaattg gactgctctc cgctctcaag tggtacatag
     1201 acggtttttc cgaacgcagc aaaatcaagg taaccgccga tatgccttcc gaccttggac
     1261 gcttgccgcg ggatgttgaa ctatctctgt ttcgcatcgt ccaggaatgc ctcacgaata
     1321 ttcaccgcca ctcaggaagt cctacggcgt ttgtgcgctt ggcccgctcc cggcaaggag
     1381 tcacccttga ggtaaaggac gaaggcaggg gcttctccgc gaatggcgaa tcgatcgtct
     1441 ccggcagcga cattcccggc gtgggattac gcggaatgag ggaaagagtc agacagcttg
     1501 gcgggcaatt tgagatcacc tcgaccggta aagggaccct cgtctcagcc aagctcccgc
     1561 tcgcagagga tttttcatcg accgctcacg gaaccgccga cttcacgcct ggccaaagcc
     1621 agtggaaaaa ctaatgcatc ggccggcctc ggcctgctag ttgacttctt taactgattc
     1681 catcaagcca aagaatcctt cagccagggt cgggtggatg aacaccgtgc ctttgaggat
     1741 tgtgtacggc gcgcccgcaa gcatcaccgc gccaagtagc tgtaccagtt cgcctccctc
     1801 agcgcccagg attgctgcgc caaggatttt gtcggtctgc gcgtccacga taatcttcat
     1861 gaatccagcc gtctcgccgc gctctatcgc gcgcgcaacc tgcgacattg aatacttgcc
     1921 catttttaat cggcgtcccg ccgcgcgagc ctctttctca gtcataccga cgcgccccag
     1981 ttgtggatcg gtaaaaagcg aatagggcac gtaacgattc tcagtggtca tattcttgga
     2041 ttggatgaga ttcgcgtaca cgatctgata gtcgttgtac gaaatatgtg tgaacgcggg
     2101 ccctcctttc acgtcgccca gcgcccagat tcccggagca gtggtttcca gccgcccatt
     2161 cacttttatg tacccgcgcg agtctatctc tacacctgcc ttgtcaagcg ataggtcatc
     2221 ggtgtttggc cgccttccgg tggcggcaag aacatgggtt ccatctatcg tggatgtttg
     2281 gtttccgctc gtgaccgtca cgcgaacctg cccctgcttt cgttcaatct ccaccggatt
     2341 cgattgcagc cggaattcga ttccttccgc ttcgagtgcc ttttgaaggt cggtcgtaac
     2401 atccgaatcc tcgtgcggca ataggtgttc ggcgcggtga atcaccgtca ctcggctgcc
     2461 gaagcggcga aacatctgcc caaactcaag gccgatgtag cctccgccca gaaccagcag
     2521 gtggtccggc acctcttgta actccatgat ggactcattg gtgagatacc ccactgcatc
     2581 gagaccgttc agcttaggcg ggtcggtgcg agctcctgaa tttataaata tgcgttcgcc
     2641 ttcaatcaca tccttgtcaa tttgaactgt tcgggcagaa agaaactttg ctactccgcg
     2701 atagaagcgc accttgtcgt gtttggctat gcctcgctcg atgccgctcc taaacctctc
     2761 cacaatggcg ttcttccgcg agacgattgc gggcaaatca acgctcacgg agcctgcctt
     2821 tacgccccat cgggacgcgt tgcgggcata gtgggcaact tgtgccgacg caatcatcgt
     2881 ctttgtgggc gtgcagccag tgttgatgca agtgccgccc acgtgagttt tttcaataag
     2941 ggccaccgcc cagtcgcgct ccgccagagc gttagctagc ggatttcctg cctgtcccga
     3001 accaattatg atggcgtcat atttcattcc ccacctcgca aattgtctgc tggttggtca
     3061 ttttggaata acgcggaggt ctgaaacgaa gcggcgggtt tttcagatgc ttcgtacctg
     3121 taaatttcaa cagcacctaa ccgtaaggtt ggctaatccc cagaggacga ggcttttggc
     3181 gcgtaatatc cggttcgcgt ccgcactaga agtttgcccc gtccgccagg tggggttacc
     3241 tttagcgcta cggaccggaa actcccgtcc ttcgctgtgt cggtcgggta gtagccaata
     3301 gtgtactgat ttcggatttc ttgcgcgatc tgctcgcaga caggagtcac atcttccaaa
     3361 ttgtcgggaa agtaagcttg gccgcctgtt gcttcggaaa gctccgccaa gacttttttc
     3421 gaatgccgaa ccattttctt atcggttttc agatcgtctt ggctgaaaac tcccaccgca
     3481 tagattgtag cgttggattg ctccgcggcc ttcaccgcgt cttcaaacga cttttcactt
     3541 gcatcatcgt cgccatcggt aactaccagc agcactctct tatccttgtg ccccttcttc
     3601 aggtgaccta gcgatccgat taccgcgtcg
//
LOCUS       DATNDT010000379         2793 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3274345, whole genome shotgun sequence.
ACCESSION   DATNDT010000379 DATNDT010000000
VERSION     DATNDT010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2793
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274345"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..836)
                     /gene="fabD"
                     /locus_tag="VN788_12360"
     CDS             complement(<1..836)
                     /gene="fabD"
                     /locus_tag="VN788_12360"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HXU21365.1"
                     /translation="MSKLAFLFPGQASQYPGMGRDLAENFPESRAVFDEADKALGFAI
                     SKLCFEGDEETLKLTENQQPAILTVSVAAYRALESRGIAPDFAAGHSLGEYSALVAAG
                     ALEFADAVKLVRKRGRYMQEAVPAGEGAMAAILGLSPADVAEVCKKAAEKEVVSPANL
                     NSPEQTVIAGSAAAVKRAVEIASQSGAKRAVILAVSAPFHCAMLMPALRRLEPDLRAA
                     KFGELRFPVITNVDAEAVSTGDEARDALIRQVTLPVRWLDSIREMIEQGVKVFVEVGP
                     GKV"
     gene            complement(860..1876)
                     /gene="plsX"
                     /locus_tag="VN788_12365"
     CDS             complement(860..1876)
                     /gene="plsX"
                     /locus_tag="VN788_12365"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HXU21366.1"
                     /translation="MTTIALDAMGGDHAPRVEVEGAILAARELGVRVLLVGGEQAVRH
                     ELARHRHRDLSIEIVNATDVITMTDSPSQAFRRKKESSVHVAARLVRDGKADALVSAG
                     NTGAVMTVARFVIGTLPSVDRPALAAAFPNMKEKISVILDVGANVDSKPAHIEQFAVM
                     GEIYYRAIWGVKRPRVALLSIGEEEAKGNELTREAAGLLKQLPLNFTGNVEGRDVFRG
                     NVDVVVCDGFIGNVALKLSEGLVEHIGAMLKKAIKSSLTSKIGYALSKRAFDDFRKRT
                     DYSETGGAPLLGVRGITVIGHGRSNPNAIKNAIRVAAELCRARVNEKIEQELSAAAAI
                     PARG"
     gene            complement(1911..2099)
                     /gene="rpmF"
                     /locus_tag="VN788_12370"
     CDS             complement(1911..2099)
                     /gene="rpmF"
                     /locus_tag="VN788_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HXU21367.1"
                     /translation="MPNPKRRHSKRRTSTRRAHDHLHPLSLAECPNCHEKKLPHRACL
                     KCGYYNGREVIDTAAKTA"
     gene            complement(2162..2695)
                     /locus_tag="VN788_12375"
     CDS             complement(2162..2695)
                     /locus_tag="VN788_12375"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HXU21368.1"
                     /translation="MFLDVHELAIRKIRIRKSYAPGTVDFHSSEFRQIEPLEVRATAE
                     LVDGQIRICGNLHTRVELVCARCLDTVVEEISRDFDLFYRPMTSIARDDELHLNLDDT
                     EIAFFEGDGLFLADILAEQVNLALPMKVICSGDCRGLCPHCGANLNNEECRCEKNGSD
                     ARLAPLARLKQDWFKKQ"
BASE COUNT          546 a          907 c          754 g          586 t
ORIGIN      
        1 acttttcctg gtcccacttc gacgaaaacc ttcacgccct gctcgatcat ttcgcggatt
       61 gaatcgagcc agcgcaccgg aagcgtcacc tgccggatca aagcgtcgcg cgcttcatcg
      121 ccggtgctca cggcctccgc atccacattg gtgatcaccg gaaagcgaag ctcgccaaat
      181 tttgcggctc gcagatcggg ttcgagccgc cgcagcgcag gcatcagcat cgcgcaatga
      241 aagggagccg aaacggccag aataactgct cgcttcgcgc ccgattgcga cgcgatctcg
      301 accgcgcgtt tcaccgccgc tgcgcttccc gcgatcaccg tttgttccgg agaattcaaa
      361 ttcgccggcg aaacaacttc tttctcagcg gccttcttgc agacttcggc cacatccgcc
      421 ggagaaagcc ccaaaattgc ggccatcgcg ccttcgccgg cgggaaccgc ttcctgcatg
      481 taccggccgc gcttccggac cagtttcact gcatccgcaa attccaaagc gcccgcggcc
      541 accagcgcgg aatattcccc caaactgtgc cccgcggcaa aatccggcgc gatccctcgg
      601 ctttcgagcg cgcgatacgc cgccacggaa acggtcagga tcgcaggctg ctggttttcg
      661 gtgagcttca gcgtctcttc atcgccctcg aagcacagct tggaaatcgc gaagcccagc
      721 gctttgtccg cttcgtcgaa cactgcgcgc gactccggaa aattctccgc gagatcccgg
      781 cccatgcccg gatactgcga cgcctgtcct ggaaaaagga acgccagctt gctcatgatg
      841 atttgcaatt tccaaaaact cagccgcggg cggggattgc tgcggctgcg gaaagctcct
      901 gttcgatttt ttcgttcacg cgggcgcggc aaagttcagc cgccacccgt atggcgtttt
      961 tgatggcgtt cggattcgac cgcccgtgac caatcaccgt aatgccccgc acaccgagca
     1021 agggagcccc gccggtctcc gaataatcgg tccgcttgcg aaaatcgtca aacgcccgct
     1081 tcgaaagcgc gtagccgatt ttcgacgtaa ggctgctctt gatcgctttt ttcagcatgg
     1141 ccccgatatg ctccaccaaa ccttcgctga gtttcagcgc cacgtttccg atgaatccgt
     1201 cgcaaaccac cacgtccaca ttgccgcgga aaacgtcgcg cccctccaca tttcccgtaa
     1261 aattcagcgg cagttgtttc aataggccgg ccgcctcgcg agtcaactcg ttgcccttgg
     1321 cttcttcttc tccgatggaa agcagcgcca cgcgaggccg cttcactccc cagatggcgc
     1381 ggtaataaat ctcgcccatc accgcaaatt gctcgatgtg cgcgggcttt gaatccacgt
     1441 tcgcgcccac atccaaaatc accgatattt tttccttcat gtttggaaac gccgcggcca
     1501 gcgccggccg gtccaccgaa ggcagcgtgc cgatcacgaa ccgcgccacg gtcatcaccg
     1561 cgcccgtgtt ccccgcgctc accagcgcat ccgccttacc atcgcggacc agccgcgccg
     1621 ccacatgcac ggagctttcc ttcttccgac ggaacgcctg cgacggcgaa tcggtcatgg
     1681 taatcacgtc cgtcgcattc actatttcga tggagagatc gcggtgccgg tggcgcgcca
     1741 gttcgtgccg gacagcttgc tcgccgccca ccagcagcac ccgcacgccc agctcgcgcg
     1801 ccgccaggat cgctccttcc acttcgacgc gaggcgcatg gtcgccgccc atagcatcta
     1861 gggcaatagt agtcatgtaa gcaccgcttg gactggcagg cgagggaaac ttaggccgtc
     1921 ttggcggcag tgtcaattac ttcgcggcca ttgtagtagc cgcacttcag acacgctcgg
     1981 tggggcagct tcttttcgtg gcaattcggg cattcagcca gcgaaagcgg atgcagatgg
     2041 tcgtgcgcgc ggcgggtgga tgtccgcctc tttgagtgtc gtcgtttcgg attaggcatg
     2101 gtgctcctcg tggcaggccg cgactcaatg cggcctgaat gcaatcaaac agtgtagcgt
     2161 tttattgctt tttgaaccag tcctgcttca gtcgagccag cggggcgagc cgggcgtcgg
     2221 agccgttctt ttcgcagcgg cattcctcat tattcaggtt cgcgccgcag tggggacaca
     2281 gcccgcggca gtcgcccgaa caaatcactt tcatgggaag cgcgaggttc acctgctccg
     2341 ccagtatgtc cgcgaggaaa agcccgtcgc cctcgaagaa ggcgatttcc gtgtcgtcga
     2401 ggttcagatg caattcgtcg tcgcgcgcaa tagaggtcat cgggcggtag aaaagatcga
     2461 agtctcggct gatttcctcg accactgtgt ccaggcagcg tgcgcagacc agttccaccc
     2521 gcgtgtgcag attcccgcag atgcggatct gcccgtccac aagttcagcc gttgcacgca
     2581 cttccagggg ttctatctgg cggaattcgc tggaatggaa atcgactgtt ccgggagcat
     2641 agctcttccg aatacggatt ttgcggatgg ccagttcatg tacgtcgaga aacattgcaa
     2701 ttttcctata atgtccactg cgaaattcat tatatgtccg ttactcgcga tgtcaagaaa
     2761 cgctcgccct ccgttgccgc aaagcacatg aaa
//
LOCUS       DATNDT010000380         4301 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4075738, whole genome shotgun sequence.
ACCESSION   DATNDT010000380 DATNDT010000000
VERSION     DATNDT010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4301
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4075738"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2007)
                     /locus_tag="VN788_12380"
     CDS             complement(<1..2007)
                     /locus_tag="VN788_12380"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HXU21369.1"
                     /translation="MNSIANSKEVAAPGFPLNAEQRAAVEHGEGPLLVIAGAGTGKTR
                     VITERIRRLLESEPSLSGENILGLTFTDKAAGEMQSRVVKSCGKRAEGIWLSTFHSFC
                     LEKILRASNPEIQAIEEIDHQILLRRNLAELGLVIFRRLAEPAQFLKDFVAFFSRCQD
                     ELVTPDDYQRYVGGLRRGHGARKIGMEADALQVAEEELAKQDELARVYRVSERLLLER
                     NLYTFGAQLLKSVECLRTDSQLLEGLRNQYRYILVDEFQDTNIAQLELLWLLSGERRN
                     ILAVGDDDQAIYRFRGASFGSFTIFLERFCGVEGKQRGAAAKDFLVSLSQNYRSTQRI
                     LRVAGEVIGVNERSPLLPAKKLVTENPNGEKIRVAEFGGFDEEAEWVAGEIERLHEAG
                     ASWRTFAVLYRKHRHRAHLLGALRRRRIPFVIRKSSILSSTLVRDLLAWMRLVAVPAD
                     NVACARVLGAPYWGLEARDLVRLAERATKNRRPLWDQVEASQKEAPLDREGIRLPELV
                     QLFNQLRQAAAKKRAVEVLDELIANLGIGMLMSSIDRQHVEKLHAFVKEWEGKSEKKQ
                     LRDFVEYLGLFDELDGDIQLEEELSDDAVQLMTVHSAKGLEFPHVFILRLSNNDFPSK
                     PRPATFEFPRELMKEEQPKYDFQTQEERRLFYVALTRAQKRLTLS"
     gene            complement(2066..3094)
                     /gene="lpxD"
                     /locus_tag="VN788_12385"
     CDS             complement(2066..3094)
                     /gene="lpxD"
                     /locus_tag="VN788_12385"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013568471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HXU21370.1"
                     /translation="MKLLALAEKLDCEVEGDANIEITGVAGIEEAHAGELTFLVNRKY
                     RPALDTTRASAILIGREEARPRMAALRSANPYLDFARAIEIFHPTPRYGASIHRTAVI
                     GESAKIGTGAHIGPYCYVAEDVEIGANAVLHSFVCIYRGARIGDDFFAHSHACVREGC
                     RIGNRVILQNGVVVGSDGFGFARDASGRWHKMRQAGITVIGDDVEIQAHSAIDRATVG
                     ETQIGRGTKIDNLVQVGHACKVGEDTLLCGQVGLAGTTHVGNRATLAGQVGAAGHLSI
                     GDGATLTAQSGVPTDIPPDAIFSGYPAMNNLDWRKSVAVFNRLPQLQKELRELRAEVA
                     QLRSERRT"
     gene            complement(3558..>4301)
                     /locus_tag="VN788_12390"
     CDS             complement(3558..>4301)
                     /locus_tag="VN788_12390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21371.1"
                     /translation="PGDFKAGAMPARNTASYQPPPRTNKPNENRPNNGNFNRPNNGAF
                     SRPENGNRPENRNVNPPNNMNRNRPETRPNENAPVNPNANRPENRNVNPPNNMNRNRP
                     ENRPNPTVNPNENRPENRNVNPPNNMNRNRPENRPNENPPVNPNVNRPENRNVNPPNN
                     MNRNQPENRNMTRPNNPNETRPNEVNRPNRENQPHNQPRNNAQPNRNVQPEAPRSQPQ
                     QEQRAAPQSRPQPERRAEPEHKPKSDNEH"
BASE COUNT          807 a         1275 c         1194 g         1025 t
ORIGIN      
        1 ggaaagcgtc agccgctttt gcgcgcgcgt cagcgccaca taaaatagcc gccgctcctc
       61 ctgtgtctga aaatcatatt tcggctgttc ctccttcatg agttctcgcg gaaattcaaa
      121 cgtggccggg cgcggcttcg acgggaaatc attattgctg aggcgcaaga tgaaaacgtg
      181 cgggaattca aggcccttgg cggagtgaac cgtcatgagt tgtaccgcgt cgtcggaaag
      241 ttcctcttcc agttggatat ccccgtcgag ttcatcgaag agaccgagat actcgacgaa
      301 atcgcggagt tgtttcttct cgcttttccc ctcccactcc ttcacgaagg cgtgcaactt
      361 ttccacgtgt tggcggtcga ttgaggacat cagcatcccg atgcccaggt tcgcgatcag
      421 ttcatcgaga acctcaacag ctcgcttctt cgcggctgct tgcctcagtt gattgaagag
      481 ttgcacgagc tccggcagcc gaatgccttc ccgatctaga ggagcttcct tttgcgatgc
      541 ttccacctga tcccaaagcg ggcgccggtt ttttgtggcg cgttcagcca gacgcaccag
      601 gtcacgcgcc tccaatcccc aatacggagc gcccagcacc cgcgcacacg ccacgttatc
      661 cgcagggacc gcaaccagcc gcatccacgc cagcaggtcg cggacaagcg tgctcgataa
      721 aatcgaggac ttgcgtatca caaacgggat tctccgtcgt cgcagcgctc ctagaaggtg
      781 cgcgcggtgc cgatgcttgc ggtagagcac tgcaaaagtt cgccacgagg ctcccgcctc
      841 atgtagccgc tcaatttccc cggctaccca ttccgcttct tcatcgaacc cgccgaattc
      901 ggccacccga atcttttcgc cattcgggtt ttcagtcaca agttttttcg ccggcaacaa
      961 aggtgaccgc tcgttcacac cgatcacctc gcccgccaca cgaaggatcc gctgcgtcga
     1021 cctgtagttc tgcgagagcg ataccaaaaa gtccttcgct gcggcgccgc gttgctttcc
     1081 ctccacaccg caaaatcgct ccaggaaaat cgtgaagctg ccgaaagatg caccccggaa
     1141 gcgatagatc gcctgatcgt cgtcgcccac ggccagaatg ttgcggcgtt ctccggacag
     1201 cagccagagc aactcgagtt gcgcgatatt cgtatcctgg aattcgtcca ccaaaatgta
     1261 gcggtattgg ttgcggaggc cttccagcaa ctgagaatcg gtccgcaaac actccaccga
     1321 cttcaacagt tgcgccccga aggtatagag gttgcgttcg agcagcagcc gctcgctcac
     1381 gcgatagact cgcgccagtt catcctgctt cgccaattcc tcttcggcta cttgcaacgc
     1441 gtccgcttcc atgccaatct ttcgcgcccc gtgcccccgt cgtagcccac ccacgtatcg
     1501 ctggtaatca tccggagtca ctagctcatc ctggcagcgc gaaaaaaaag ccacgaagtc
     1561 tttcaggaac tgcgccggtt ccgcgagtcg ccggaagatg accaatccga gttccgcaag
     1621 atttcgccgc agcaaaatct gatgatcgat ttcctcgatg gcctgaattt ccggattcga
     1681 cgcccgcaaa attttctcca ggcagaacga gtggaaggtg ctgagccaaa taccttctgc
     1741 tcgctttccg caggacttca caacgcggct ctgcatttct ccggcggctt tgtccgtgaa
     1801 ggttagaccc aaaatatttt cgccgctcag cgacggctcg ctttcgagca accggcgaat
     1861 acgttcggtt atgacgcgag tcttccccgt gcccgcaccg gcgattacca gcagcggacc
     1921 ttcaccatgt tcgaccgcgg cccgttgctc cgcgttgagc ggaaaaccgg gcgccgctac
     1981 ttctttcgag ttcgcgattg aattcataag cagtgctctg ctgattgtat caggtttcac
     2041 gcatggcgaa cataaagcga aaattctacg tccgccgttc gctgcgaagc tgcgcgacct
     2101 ccgcccgcag ttcgcgcagt tccttctgta attgcggcaa acggttgaag acggcgaccg
     2161 atttgcgcca atccaaatta ttcatcgcgg gatagccaga aaaaatcgcg tcgggcggaa
     2221 tatcggtggg gacgccgctc tgcgccgtca gggtcgcgcc gtcgccaata ctcaggtggc
     2281 ccgctgcgcc aacttgcccc gcgagagttg cgcggttgcc gacatgcgtc gtgcccgcca
     2341 gtccaacctg accgcacagg agggtgtctt ctcccacttt gcaggcgtgg ccgacctgca
     2401 ccaggttgtc aatcttggta ccgcggccga tctgagtttc tccgactgtg gcgcggtcga
     2461 tggcgctatg ggcctggatt tccacgtcgt cgccaatcac tgtaatcccc gcttggcgca
     2521 tcttgtgcca ccggccgctt gcgtcacgcg cgaaaccaaa tccgtcgctg cccaccacca
     2581 cgccgttctg caaaatcacg cgattgccga tgcgacaccc ctcgcggacg cacgcatgcg
     2641 agtgagcgaa aaaatcgtcg ccaattcgag cgccgcgata aatgcacacg aagctatgca
     2701 gcaccgcgtt cgctccgatt tcgacgtcct cagccacata gcaataaggc ccaatgtggg
     2761 cgcccgtccc aatttttgcg gactctccga ttactgccgt gcgatgaatg ctggcaccgt
     2821 agcgcggcgt gggatgaaaa atctcgatgg ctcgcgcaaa atcgagatac ggattcgccg
     2881 accggagcgc ggccatgcga ggacgcgctt cctcgcgacc tatcaggatg gccgaggcgc
     2941 gcgtcgtgtc caaggctggg cgatatttgc ggttgacgag gaacgtcaac tcgcccgcgt
     3001 gcgcttcttc aattccggcg acgccggtga tttcgatgtt cgcatcacct tcaacttcgc
     3061 agtcaagctt ctcggcgagc gccagcaatt tcatggtgat ctcctgagcg tttcagacga
     3121 tgttccgaat acttcgagtc tctgctgctt ttagcgcccc ctcgtaaaag tgtcgagtag
     3181 aacgtgcacc gatcctttgt tttgaatgag ttacgtcatg ttctactcgg ctcatgtgtc
     3241 gattagaact gccctggccg cccaccagga gggctcccga ggagattccc gaggccctcc
     3301 gaccccccca tttcgcgccg aatttttcga gtttattcac ggcgtccatg ttaggcgcta
     3361 ggctggcgaa ttcaagcggt tgttggtgta aagaatttac agctgtgcag atcgcttcgg
     3421 cggcctgatc gcgccttctg gcgggcattg aaagctaaca gttgagaatc gaaattcaaa
     3481 cggaaggaga aggaggctga cggaaggcgg ggttggccgc cggctctctt ggagagagcc
     3541 ggcgtcaacg cgagagtcta gtgctcgtta tctgacttcg gcttgtgctc gggctcggct
     3601 ctgcgctcgg gctgtggtct ggactgaggc gcggcccttt gttcctgctg cggctgactt
     3661 ctcggcgctt ccggctgaac gttccgattt ggctgggcat tgttgcgcgg ctgattgtgt
     3721 ggctgatttt ctctgttcgg tcggtttact tcgttcggac gagtctcgtt cggattgttc
     3781 ggacgggtca tattcctatt ttccggctga ttacggttca tgttgttggg aggattcacg
     3841 ttcctgtttt ccggacggtt cacatttggg ttaaccggcg gattctcgtt cggacgattt
     3901 tctgggcggt tgcggttcat attgtttggg ggattcacat tccggttttc cggccgattc
     3961 tcatttgggt taaccgtggg attcggacgg ttttctggac ggttgcggtt catattgttg
     4021 ggaggattca cgttccggtt ttccggacgg ttcgcatttg gattaaccgg ggcattctcg
     4081 ttcggacggg tttctggacg gttgcgattc atattgtttg ggggattcac gttccggttt
     4141 tccggacgat tcccattctc cggtcggctg aaggcgccgt tattcggccg attgaagttc
     4201 ccattgttgg ggcgattctc attcggctta ttggtgcgag gaggcggttg gtacgaagca
     4261 gtgtttctgg cgggcatcgc tccggctttg aagtcgccag g
//
LOCUS       DATNDT010000381         9426 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3257030, whole genome shotgun sequence.
ACCESSION   DATNDT010000381 DATNDT010000000
VERSION     DATNDT010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9426)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9426)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9426
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3257030"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..1377)
                     /locus_tag="VN788_12395"
     CDS             complement(7..1377)
                     /locus_tag="VN788_12395"
                     /inference="COORDINATES: protein motif:HMM:NF017893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane CreD family protein"
                     /protein_id="HXU21372.1"
                     /translation="MTKRIIAIAVIYIFSTIAWAILGATVFIRTNASNSSLRNSVASL
                     WGAPQEQTPPEAWYSTVEASPAPTTTTASSPVTKSVQMPIDKSRVQAALHLDYRKKGL
                     LWYSTYTVRFAGAYDFRNDSDQPRRVVFSLNFPTAQAIYDDVTFSVNGAPLATSNAKT
                     QAYAPYTIPPHQTATFQVGYRSQGLEKWTYNFGDQVNQVKDFELHLTTDFSGFDFPDN
                     SLSPTDEHRDGSGWTLVWNYKNLISGYKIGLAMPEKLQPGPLTGRISFFAPVSLLFFF
                     FILFVLTTVRQIDLHPMNYFFLACAFFSFHLLMAYLVDHLSVNAAFLICSAVSIFLVV
                     SYLRLVVGMRFAAFEAGVAQLIYLVLFSYAFFFEGFTGLAVTIGAIITLFVVMQVTGR
                     IRWSEQFARKPVRAEVCFAAEAEASGLAETRQTRATLRARWWQDREQRKRGPSPRRFA
                     DEPQSP"
     gene            1587..2087
                     /locus_tag="VN788_12400"
     CDS             1587..2087
                     /locus_tag="VN788_12400"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HXU21373.1"
                     /translation="MKRKWMRWLIGVAVFFVVIQAIRPARTNPPIDPSQEIAAKLPID
                     PAVQAIFARSCNDCHSQRTVWPWYSNIAPVSWLVIRDVDDGRRHMNLSNFTIAPPRRL
                     AHTLRNVCSEVRSGDMPMTIYVPMHPKSRLTSADVEAICQWTQARLNALPPDALSDRR
                     APRGPE"
     gene            2733..4610
                     /locus_tag="VN788_12405"
     CDS             2733..4610
                     /locus_tag="VN788_12405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006498597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein DipZ"
                     /protein_id="HXU21374.1"
                     /translation="MLLLMLAFAGGILTIVSPCILPVLPFVFSRADQRFRESGLPLLA
                     GMVLTFAVMASLAAAAGTWIVRANQIGRYAALVIFAVLGVSLLFPAVAEYLSRPFVRL
                     GCKVQGHNAETPSVGRSVLLGISTGLLWAPCAGPILGLILTGAATQGASLHSLFLLTS
                     FAAGAACSLAVVVLVGGRVLNALKRSLGVEEWIRRGIGVAVLLGVIAIAFGWDTGALA
                     RISLGETSGFEQGLVDHVRPAPQETASNVSADAVMSLKANTAPDPAANSPASVVSSDG
                     ALSMKAVNTGAAMISMKGSSSALPAIPVGNEGSLPSLNGAIAWLNSPPLTREALAGKV
                     VLVDFWTYSCINCLRSIPYVEAWAEKYRAAGLVVIGVHTPEFAFEKDLSNVRKAVKDL
                     KITYPVAVDSNYAIWKAFHNEYWPAHYFIDANGYIRYHHFGEGEYDKSEGVIQQLLKE
                     KNSNLQLSGLVNVKGSGAEAAPDMNDVESPETYIGYERQENYVSPEKIASGVPANYTA
                     PGRLDVNNWALAGQWNVEAEKAALVSAPGKIVFRFHARDLHLVLGPGPNGKPVRFRVL
                     LDGTAPMDDKGVDVDSGGNGTVTGYRLYQLIRQKGKVEDRTFEIEFLDPGVQAFSFTF
                     G"
     gene            4761..5207
                     /gene="msrB"
                     /locus_tag="VN788_12410"
     CDS             4761..5207
                     /gene="msrB"
                     /locus_tag="VN788_12410"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008953036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXU21375.1"
                     /translation="MVSIVMFSNAGVRERVVSLPKVVKSDAGWRKQLGYDSFEVTRHG
                     DTERPFTGALLNEHQKGIFCCICCDTAVFSSDTKFESGTGWPSFWAPIAKENIVETTD
                     TGFGMIRTAISCRLCDAHLGHVFNDGPKPTGLRYCMNSVALKFAKA"
     gene            6109..6471
                     /locus_tag="VN788_12415"
     CDS             6109..6471
                     /locus_tag="VN788_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009011543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HXU21376.1"
                     /translation="MFKLKRIYAAPESADGYRVLVDRLWPRALSKDKAHLDLWLKDIA
                     PTNELRKWFGHDPKRWPEFQKRYRAELRKNSVLVKQLKEIEKQHGTVTLLYSAHDELH
                     NQAVALYSLLVPRSHQPD"
     gene            6777..8852
                     /locus_tag="VN788_12420"
     CDS             6777..8852
                     /locus_tag="VN788_12420"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HXU21377.1"
                     /translation="MNSRISYGVTIFVGAFLLFEIEPLIAKRILPWFGGAAAVWIICL
                     LFFQLVLLLGYAYAHWLTSKFATSMQSRIHAALLILSVLFLPVYPKSSWQPTPTQSPT
                     SHILLLLTATIGLPYFLLSATSPLFQSWYATQHKAAPYRFYSLSNIGSMLALLSYPVA
                     VEPWLTTHHQAVAWSMIYAVFVILCIPIAFSRRGPTETLENASVTPRPSWPLQLLWVS
                     LAACGSALLLAITNHITQNIAAVPLLWIIPLSLYLLTFILCFASHSFYPRSVFLRLLA
                     VVLGGMTYALSPSFLQMPVKIAIPLFCAGLFVACMFCHGELARLKPDPQHLTSFYLMV
                     AAGGALGAIFVAVIAPHIFSAFYELHVAIGMCAILVVIVYAIEPESPFSVAKLRPGGF
                     ALAALAIAIAASLIVNARNYAISAQLTVRNFYGVLRVMDGSAPNVAIIRGNSISQVGE
                     KPAYRSLVNGTINHGAQFLAPDRRRWATTYYSHDSGIGVALQAAEKTRPVRVGIIGLG
                     AGTTAVYGRSGDSFTYYEINPLVIQIAQQEFTFLKDSPATINIVQGDARLSLERQPPQ
                     AYDVLVVDAFSGDSIPVHLLTREAFQLYFQQLKPDGVLAVHISNSYLDLEPVVASAAA
                     SLRKEAVVVHNSNDPPKGIFASIWVLVGSPYGFEGRREIEAKGLIILPPSKDRLWTDD
                     YSNLFEVLR"
     gene            8954..>9426
                     /locus_tag="VN788_12425"
     CDS             8954..>9426
                     /locus_tag="VN788_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXU21378.1"
                     /translation="MKEFVPHWALRNSHLMTIAAHLWRRRFPSLPAGEPRLFETEPGT
                     RVRATCHWRQNPQAHPTLVLLHGLEGSSHSGYVMGTAEKAWLAGFNVIRLNQRNCGGG
                     DDLTHTLYHSGLSCDIRAALIELIQKDRLTNLFAAGFSMGGNLVLKMAGEFGKATP"
BASE COUNT         2021 a         2658 c         2537 g         2210 t
ORIGIN      
        1 atttcgtcac ggactctgag gctcgtccgc gaagcggcga ggcgaaggac ctctctttct
       61 ttgctcgcga tcctgccacc aacgagcacg caatgtagct cgggtctggc gcgtctctgc
      121 cagacccgag gcttctgctt ctgcggcaaa gcaaacttct gctctcaccg gtttcctcgc
      181 gaactgctct gaccatcgaa tccgccccgt cacctgcatc acaacaaaga gcgtaatgat
      241 cgcaccaatc gtaaccgcga gtccggtgaa gccttcgaag aaaaacgcat aggaaaacag
      301 caccaggtaa atcaattgcg ccacgcccgc ttcaaacgcg gcgaatcgca tgcccaccac
      361 gagccgcaaa tacgagacga ccagaaatat cgatacagcc gaacatatga gaaacgccgc
      421 attcaccgac agatgatcca ccagatacgc catcagcaga tgaaacgaga aaaacgcgca
      481 cgccagaaag aagtaattca tcggatgcaa gtcgatctgc cgaaccgtcg ttaatacgaa
      541 tagaatgaag aagaaaaaga gcaacgaaac aggagcaaaa aagctgatcc ggccagtcag
      601 cggtcctggc tgaagttttt cgggcattgc gaggccaatc ttgtatcccg agatcagatt
      661 cttgtagttc cacaccagcg tccaacccga gccatcgcgg tgctcgtccg ttggcgagag
      721 tgaattatcc ggaaaatcga aaccggaaaa atccgtcgtc aggtgtagct cgaaatcctt
      781 cacctgattc acctgatctc caaagttgta cgtccacttc tcgaggcctt gcgaccggta
      841 tcccacttga aacgtggcgg tctggtgcgg aggaatcgta tagggcgcat acgcctgggt
      901 ctttgcattg gaagtggcaa gcggcgcgcc gttcaccgaa aacgtcacat cgtcataaat
      961 cgcctgtgca gtagggaagt tcagcgagaa gaccacgcgc cgcggctgat cgctgtcgtt
     1021 ccgaaaatcg tatgcgcccg cgaatcgcac tgtgtacgtg ctgtaccaga gcaatccttt
     1081 ctttcggtag tccagatgta acgcagcctg cactcggctc ttgtcgatcg gcatctgaac
     1141 ggactttgta accggcgaag aggcggtggt cgttgtcggc gccggcgagg cctcaaccgt
     1201 gctgtaccac gcctccggtg gcgtctgttc ctgcggagcg ccccagagcg acgcgacgct
     1261 gttgcgcaga ctcgaattgg aggcgttcgt gcgtatgaaa accgtcgccc cgagaattgc
     1321 ccatgcgatg gtgctgaaga tgtagatcac ggctatggcg atgatgcgtt tggtcattgg
     1381 attcctctca agtgaagtcg cacggccgcc gcaacaccgc tactgtgcca taaataagat
     1441 ggtgtagtaa agcactttat gttacagagt aggcgtactg gtaaaggctt gggattgcgt
     1501 tgtaagcgac acagtgacac tctcgcgcgg tcgccttaca aaaacgccgg gtgatggcgt
     1561 aggatgctcg tttcttaagg agatctatga aacgcaaatg gatgagatgg ctgattggcg
     1621 ttgctgtctt cttcgtcgtg atccaggcga tccgtcccgc acggacgaat cccccgatcg
     1681 atccttctca ggaaatcgcc gcgaaactgc cgatcgatcc ggccgtgcag gcaatcttcg
     1741 ctcgctcttg caacgactgc cattcccagc gcaccgtttg gccctggtac agcaacattg
     1801 cgccggtctc atggctggtc attcgtgacg tggacgatgg ccgccgccat atgaacctat
     1861 cgaatttcac gatagcgccg cctcgccggc tggcccatac gctccgcaac gtctgtagcg
     1921 aagtgcgctc cggcgatatg cccatgacca tctacgtccc catgcatccc aaatcaaggc
     1981 tcacttcggc cgatgtcgag gcaatttgcc agtggaccca ggcgcgcctc aacgcgctcc
     2041 ctcctgacgc cctctcggac cggcgcgccc cacgcggccc cgagtaagct cttctcgcga
     2101 agcggctact tgtccgtgta gccgtaccgg ttctctagcc gctcatgccg gtctgatgct
     2161 acggtccgca gattcctggg ccatgacaaa ccactccggc ttagtgtaga ctcgcgtcca
     2221 aatttcctgg cgaggccgta tggtccgtcg aattcccgct gtttcatacg aggtcgaatg
     2281 tttgctgctt tccggttgtg ccggtgtcgt cccgttgcct actcacaccg aaactccggc
     2341 tcacggtaca gatcaaacag aaagtaggcg caagtttcgt aattccagga caaacaacca
     2401 aagaagaggt cttggccaag ctgcaaccgg tcaactctgg gataaatggt gttcgcctct
     2461 ttcttgcacg ctgctccagt tcgaacaagg gcggatttgc tcttctgtgt ggttacgtca
     2521 gttgcttggg tggcggatca cgtctttgga agacctcgaa tgcgctactg gaattcgacg
     2581 aacacgattt gctgcagcga tactccgttt ttggcgacaa cattctagtc accaaacttt
     2641 cgccattgcg agccaaaacg caatttatat acggaagaga ctttttccag cggcccgagt
     2701 gatcggttcg caggtcatcg gagagtgagc gaatgctctt gctaatgttg gcgtttgccg
     2761 gcggcattct cacgatcgtc agcccatgta tcttacctgt gcttcctttt gtgttttcac
     2821 gcgctgacca acggtttcgt gagagcggtt tgcctcttct tgcaggcatg gtattgacct
     2881 ttgccgtgat ggcgagcctt gcggccgcgg caggaacctg gatcgttcga gctaatcaga
     2941 tcggcaggta tgcggctctg gtcatcttcg cggttctagg cgtctctctt ttgtttccgg
     3001 ctgtcgcgga gtacctttcg cgtccttttg tccgcttggg ttgtaaagtg cagggacaca
     3061 acgcggagac tccgagcgtt ggtcgatccg ttctgctggg aatatcgacg gggctgctgt
     3121 gggctccatg cgctgggccg attttgggcc ttattcttac tggcgcggcg actcagggag
     3181 caagccttca cagccttttt ttgttgacgt cctttgcggc aggcgctgcc tgctcgttgg
     3241 ctgtcgtagt cctcgttggt ggtcgtgtgt tgaacgcact caaacgctcc ttgggagtcg
     3301 aggaatggat tcggcgcggg attggcgttg cagtgcttct tggggttata gcaattgcct
     3361 tcggatggga tacgggagcg cttgccagga tttcattggg agagacctcg ggattcgagc
     3421 agggattagt cgatcatgtg aggcccgctc cgcaggagac cgcttccaac gtgtccgcag
     3481 atgcggtgat gagcctgaaa gcgaacaccg ctcccgatcc agctgcgaat agcccggcat
     3541 ccgtggtttc atccgatggg gcattgagta tgaaggcggt gaataccggt gcggccatga
     3601 taagcatgaa aggttcatcg tctgcgctgc ccgccatacc cgtcggcaac gaaggcagcc
     3661 tcccgagcct gaacggcgcg attgcctggc tcaattcgcc gccgctcact cgcgaagctc
     3721 tggccggtaa agtagtgctc gtggatttct ggacgtattc ctgcatcaat tgcctgcgat
     3781 cgattcccta cgtggaggcg tgggcagaga aatatcgagc tgccggtctc gtcgttatcg
     3841 gcgttcacac accggagttc gcttttgaga aggatctttc caacgtaagg aaggccgtaa
     3901 aggatctcaa aatcacgtat cctgttgcag tcgacagtaa ttatgcgatt tggaaggcgt
     3961 ttcacaacga atattggccg gcccactact tcatcgacgc caacggatac atccgctacc
     4021 atcacttcgg cgagggcgag tacgacaagt ccgagggagt catccaacag ctcctgaaag
     4081 aaaagaattc gaaccttcag ctcagcgggt tggtaaacgt caagggttcg ggtgccgaag
     4141 cggcaccgga tatgaatgac gtggagtcgc cggaaacata cattgggtac gaacgccagg
     4201 agaactacgt atcgccggag aagatcgctt caggagtgcc cgcgaactat acagctcccg
     4261 gaagactaga cgtcaacaat tgggcgctgg ctggccaatg gaatgttgaa gccgaaaaag
     4321 cggctctcgt ctccgcgccg ggtaagatcg ttttccggtt tcatgctcgg gatctgcatc
     4381 tggtgcttgg cccgggaccg aacggaaagc ctgtgcgctt tcgagtcctt ctggacggca
     4441 cggcgcctat ggacgacaaa ggcgtggacg tggacagtgg cggcaacggc accgtgaccg
     4501 gatatcgctt gtatcagttg atccgtcaaa agggcaaggt cgaggatcgg acctttgaga
     4561 tcgagttcct tgatccgggt gtccaggctt tttctttcac gttcggctga agaagggaca
     4621 actctatgct cgaaatgtct aaacaatctg ggaagtctcg gttggcgcgc cgcgcgttct
     4681 tggccacatc cggcgccggc ttggcgggct tggctttttg gttggccacg cctcatccag
     4741 gagaagccgc aggaccggca atggtttcga tcgtcatgtt ttccaatgcc ggagttcgcg
     4801 agagagttgt ctccttaccg aaagtcgtta agtctgacgc cgggtggcgc aagcaactcg
     4861 gctacgactc attcgaagtc actcggcacg gcgatactga gcggcccttt acgggtgcac
     4921 tgctgaatga gcatcagaaa ggcatattct gctgcatctg ttgtgatacg gctgtattta
     4981 gctcggatac gaagttcgag tcgggaaccg gctggccaag cttttgggcg cctatcgcga
     5041 aggagaatat cgtcgaaacg acggataccg gtttcggaat gattcgaacc gcaatttcct
     5101 gccggctttg tgacgcgcac ttggggcatg tctttaatga tggtcccaaa cctacaggtc
     5161 ttcgctactg catgaactcc gtggcgctga agttcgcaaa agcatgagtc tcgcgattgg
     5221 aaccttcaag gcgcggagca tacagtgaca aacagcgcag gagccaagcc tgtctggcat
     5281 tgcagcacgc ccgaagcgat actcttggcc cgacgtcgcg cagttgcgaa acgccggtcg
     5341 cgtaaggtac acaacccacc ggattgccct tcgcggcacg gcagcgtaag cccaaggcaa
     5401 tgggcggctc acagcactgg cttctctgga acttgatcgc catcgtcgtt tctttcgacg
     5461 tgctggctat tgtggtgctt gttaccgtct gcccgtttcg gctcgttcga tggcttggtc
     5521 ggatgcttta tgtgcggaaa ccggttttcg gcccagaact aaaacgcacg aatctgattt
     5581 ccttcgcgac agttgattcc cggcctatcg tgctgaagac tcctgaggtc ggccaatctg
     5641 aaaataagct gaatgcggtg cttacaatac cgagcacgtg tgcggactcc cgatcaaaaa
     5701 tttgtggcat catgcacact atggcacacg tgataccgga cgaaattact agagcggtta
     5761 gactcgctgg gtttgagccg atcgaaacca tcgagcccgc gcgccgtgtt tttacgcgcc
     5821 gccaatgcac gcttgtaatt aacgacgatg caaggtggtc ctgtcattgg caagcgccag
     5881 acgttagaag gttgtgctgc gcgggatata cattcgcgtc gctattggat tatgtgggcg
     5941 atgcagcagc gcggtataaa caggtcgggg caccgccaca cagctaacgt gattgcccta
     6001 cgcgatcccg agcaagtatt cgcattgggc tttttcagga attgatcgaa cgtttttccg
     6061 gaatctcaat tcgaaagaat ctcgcattca tcgtaaaatt cgcaaagcat gttcaaactc
     6121 aagcgcattt acgcagcgcc cgaatcggcc gatggatatc gcgttctcgt agaccgcctt
     6181 tggccgcgcg ccctctcaaa agataaggct catctcgatc tctggctgaa agatatcgca
     6241 cccacaaacg agcttcgaaa atggttcggt cacgatccca agcgttggcc cgaatttcaa
     6301 aaacgctacc gcgcagagct gcggaagaac tccgtactcg tcaagcaact caaagaaatt
     6361 gaaaagcagc acggcacagt aacgctcctc tactctgccc atgacgaact ccacaaccag
     6421 gccgttgccc tttactcact cctcgtgccg cgttcgcatc agcctgactg aggccacccc
     6481 tttcccggaa tgggaattgt tcccgaaggg tcattcatgt ggacccgtga taaatagcca
     6541 ccccgccatc gatcatcgat gggcattttc gtgattaata gggtctcgag atcagcctct
     6601 gcggatgaaa cagaaacttc gctgaaaatc gtgcgccaaa cgcgccattt ttgtcccctt
     6661 aacgccattt aagccaattt tatggcgcca aatgcgccaa aattggcaca aattcgccct
     6721 ccaaaacttg agtctttaca tgtaagatcg cggcgcggaa aaatcaatcc ggcaaaatga
     6781 attccagaat ttcttatggc gtcacgatct tcgtcggcgc atttctgctt tttgaaattg
     6841 agccgctgat cgccaaacgc attcttccat ggtttggagg cgccgcagcc gtctggatca
     6901 tctgccttct tttctttcag ttagtactgc ttttgggcta cgcctatgcg cactggctca
     6961 cgtcgaaatt cgcaacttcc atgcaatctc ggattcacgc cgcgttactg attctgagtg
     7021 ttttgttttt acctgtttat ccaaaatcct cgtggcagcc caccccgaca caaagcccaa
     7081 cctcacacat tctcctgctc ctaaccgcca ccatcgggct cccatacttt ctgctctctg
     7141 ctacaagtcc tttgtttcaa agctggtacg cgacacaaca caaggccgct ccatatcgtt
     7201 tctattcgct ttcaaacatc ggttcgatgc tcgccctcct cagctatccc gtcgccgtcg
     7261 aaccgtggct caccacgcat catcaagccg tcgcatggtc aatgatctac gccgtgtttg
     7321 taatcctctg tatcccaata gcattctccc gtcgcggccc aaccgaaact cttgaaaatg
     7381 ctagcgttac tccacgccca agctggccat tgcagctcct ttgggtttcg cttgcggcgt
     7441 gcggatcggc cttgctgctt gcgatcacaa atcacataac gcaaaacatc gcggcggtgc
     7501 cgctcctctg gatcatcccg ttgagccttt atttgcttac gtttatcctg tgtttcgcgt
     7561 cgcactcgtt ctatccgcgt agcgttttcc ttcgcttgct cgcagtcgtg ctcggcggca
     7621 tgacgtacgc gctctctccg tcatttctcc aaatgccagt aaagatcgca attcctttat
     7681 tttgtgcggg tttgttcgtg gcgtgcatgt tttgccatgg tgagcttgcg cgcctcaagc
     7741 cggatccgca acacctcaca tctttttacc tgatggttgc ggcgggaggc gcgctcggtg
     7801 caatttttgt ggctgtaatc gcgccgcaca tcttctccgc tttctatgag ttacacgtag
     7861 caatcggcat gtgcgcgatt ctggttgtga tcgtttacgc gatcgaaccg gagagtccat
     7921 tttccgtcgc gaagctgcgt cccggcggtt ttgcactcgc agctctggcg attgcaattg
     7981 ccgccagcct gatcgtaaac gccagaaact acgcgatttc cgcgcaatta acggtacgca
     8041 atttctatgg cgtcctgcgc gtaatggacg gctccgctcc aaacgtagcg atcattcgcg
     8101 gcaattcgat atcacaagtc ggcgaaaagc ccgcgtaccg ttcgctcgtc aatggcacga
     8161 tcaatcatgg cgcgcaattt cttgcgccgg accgccgccg ttgggccacc acatattaca
     8221 gccacgattc cggtatcggt gttgctctgc aagccgctga aaagacacgg cctgttcgcg
     8281 tcggaatcat tggcctaggt gccgggacga ccgccgtcta tggccgctcg ggcgacagct
     8341 tcacctatta cgagatcaat ccgctagtga tccagatcgc acagcaggag ttcacgttcc
     8401 taaaggactc gccagcgacg atcaacatcg tccaaggaga tgcgcgcctc tcgctggagc
     8461 gccagccgcc ccaagcgtac gacgttctcg tggtcgatgc gttcagcggt gattcgattc
     8521 ccgtgcacct gcttacgcgc gaagcttttc agctttattt tcagcagctt aagccggatg
     8581 gcgtcctcgc cgttcacatt tcgaatagct atctggactt ggaaccggtc gtcgcatcgg
     8641 cagcggcaag ccttcgcaaa gaggccgtag tcgttcacaa ctcgaacgat cctccgaagg
     8701 gaattttcgc ttcgatatgg gtgctcgtgg gaagtcccta cggattcgag ggacgccgcg
     8761 aaatcgaagc caaaggcctg attattctcc cgccttcaaa agatcgccta tggactgacg
     8821 attacagcaa tctctttgaa gtcctacgct aatcaggccc ctatagaagc tggtgcggca
     8881 ggttatgcag actagacggc tgggggcgcg caaatttgcg tagctagacg gtagctttta
     8941 agatggagcg gccgtgaaag aattcgtacc gcattgggcg cttcgcaatt cacacctgat
     9001 gacgattgcc gctcatctgt ggcggcgtcg atttccatcg ctacctgccg gagagccgcg
     9061 cctctttgaa acggaaccag gaacccgcgt tcgcgccact tgtcactgga gacagaatcc
     9121 acaggctcat cccacgctcg tccttctgca cggactagag ggatcgagcc actccggata
     9181 cgtaatgggc acggcggaga aggcatggct cgccggattc aacgtgattc ggttgaacca
     9241 gcgcaactgt ggcggcggcg atgatttgac gcacacgctc tatcactccg ggctaagttg
     9301 cgacattcgc gcggcgctga tcgaattgat tcagaaggat cggcttacga accttttcgc
     9361 agccggattt tccatggggg gaaacctcgt tctaaaaatg gccggtgaat ttgggaaggc
     9421 aactcc
//
LOCUS       DATNDT010000382         4103 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2999647, whole genome shotgun sequence.
ACCESSION   DATNDT010000382 DATNDT010000000
VERSION     DATNDT010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4103
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2999647"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1190
                     /locus_tag="VN788_12430"
     CDS             114..1190
                     /locus_tag="VN788_12430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HXU21379.1"
                     /translation="MTNPLVSALIDTYNQGPYIEQALVSVLEQGLSPSELEIVVVDDG
                     STDNTPAIVQKFVPRVRYIRKQNGGQASAFNAAIPETHAPIVAFLDADDWWAKNKLAT
                     VLDVFERNPEPGAVGHGYFEVHCVAAPAEIVVPQRVYRIDLANAAAARLADCGRHFLG
                     TSRLTVRKSVLAKVGPIPEELIFCADTPILTLALALGGAVLLDQPLCYYRLHAANLFA
                     SAPADAAQERKKFETLQFLLDFLPRRLAEVGVSPEAIVALLQSDQLEAGRLRLIFDGG
                     WPWETFHAESRHFRLAYQEHSNGYALFKWLVLASTLVMPPRRFYRLLRWYSEHNLRRF
                     RKLLGEPVPSPAIQARREKVGLPQ"
     gene            1200..2297
                     /locus_tag="VN788_12435"
     CDS             1200..2297
                     /locus_tag="VN788_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21380.1"
                     /translation="MRDAIIVSGWNWETFNVPERVALALARLGGKVLYCENPVSRLRG
                     RGRPLEEIEGGVHRFGPEFLGHRLNLVPFGFPQFQARMMANQILQNAAKLKLKDPLFL
                     YAHGEFFLPLCREFKRKGFFLVHICMDYPEPGQEKHIQLSDLTLVIPKSVFHQLRPKY
                     GDKIALIPQVTAMLSSDCQVGANARASDEFSAIPGPRLGYIGPVTDRLNLKVLEKMLR
                     ARPDWQFIHFGAAKCLPLPNVHVIAWRDPGKLKDVTANLDVGFMPYDCYSNKNFHCMP
                     LKVFDYFHAGLPVVSTSIVNLWEFYDTIYFGDDAEELCRAVQSALDEPADSPKKSARI
                     AIAKEHSIEGLAECLDRVLSSRGLPVRQTKC"
     gene            2364..3158
                     /locus_tag="VN788_12440"
     CDS             2364..3158
                     /locus_tag="VN788_12440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXU21381.1"
                     /translation="MSRARNFAAGLLLRSRIRFRSLQKVPVIGGSLHRLSHWLLPGQL
                     RVWSRVRQGPAKDIWLEVNPRTGQGYIQGTTEAATQRRLAEYLKPGMVFYDLGANIGL
                     FSLLAARIVGPQGRVFSFEPDFETAQRLKSNVEKNSCCNITIVEAGVWSSTRKVAFIA
                     SDSTLDRGFGRFTLETANIAGKELPCYALDDFMSTAPPPNAIKCDVEGAELEVFRGGE
                     HLIATHRPWIICEIHSAENGEAVLNLFRRFGYSVDAVDSNHLLAFP"
     gene            3180..>4103
                     /locus_tag="VN788_12445"
     CDS             3180..>4103
                     /locus_tag="VN788_12445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21382.1"
                     /translation="MLKKFRVLLLCTHPVQYGSPMWRLMAQRPELEVLVAYCSLEGAE
                     AHVDPGFGVEVVWDVPLLDNYPWVHLKNVSPRPAIGGFFGLINPGVWRLIRSGKFDAV
                     VMFTGYVCATFWIATAAAKLSGIPILFGTDAHDLSPRGARAWKTKIKKYLWPRLFRLA
                     DIVIAPSSGTVALMRSLRVQPDRVMLMPYVVHNEWWIEQAARVDRAAVRRGWNIPVEA
                     PVALFCAKLQPWKRPQDLLLAFASANVPGSYLVYAGDGPMRSELEAQAKTLGISAQTR
                     FLGFVNQSGLPRVYCGSDLLVLPSEYEPFGVV"
BASE COUNT          863 a         1150 c         1130 g          960 t
ORIGIN      
        1 cgtcaccgcc tcaaagaaat gggcgaacaa gccgccatcg acgttcgcca gtgggtttct
       61 gctgatcccg tggaagattt cgtgcgcgaa ttgaacgcgc tggttgaatc acgatgacta
      121 acccactcgt ttccgcgcta atcgacacct acaatcaagg gccgtacatc gagcaggcgc
      181 tcgtcagcgt tctggagcag ggcctttcac cgtcggaact cgaaattgtg gtcgttgacg
      241 acggttcaac ggacaacacg cctgcaatcg tacagaaatt cgtcccccgc gtccggtaca
      301 tccgcaaaca aaatggcggc caggcatctg ccttcaacgc cgccattccc gaaacgcacg
      361 ctcccatcgt cgcgttcctc gacgcagacg attggtgggc aaaaaataaa ctcgcgacag
      421 ttctcgacgt tttcgaacga aacccggagc cgggcgcagt cggacacggt tacttcgagg
      481 tccattgcgt cgcagcaccc gcggaaattg tcgtgccgca acgagtctac cgaattgact
      541 tggcaaacgc cgcagcggct cgacttgccg attgcggaag gcactttctt gggacgagca
      601 gattaacggt gcgaaagagc gttctggcaa aagtgggacc cattccggag gaacttattt
      661 tttgcgcgga tactccgatc ctgactctgg cgctcgcact cggcggcgca gtcctgctcg
      721 atcagcccct ctgttactat cgtttacacg ccgcaaactt gttcgcgtcg gcccccgcgg
      781 atgccgccca ggagcgcaag aaattcgaaa ctctccagtt tcttctcgat tttcttccgc
      841 gccggcttgc cgaggtcggg gtttcccccg aggcgattgt tgcgcttctg caatcggacc
      901 agttggaagc cggccgactc aggcttatct tcgacggggg ctggccgtgg gaaaccttcc
      961 atgctgaatc caggcatttc cgtctcgcgt atcaggaaca tagcaacggt tacgctttat
     1021 tcaaatggct ggtgctggca tcaacgttgg tgatgccgcc gcgccgtttt tatagactcc
     1081 ttcgctggta ctcggagcac aatctgcggc ggtttcgcaa attactcggg gagcctgtac
     1141 cttcaccggc aattcaagcg cgccgagaaa aagttggttt gcctcaatga agggacacaa
     1201 tgcgtgacgc catcattgtg tccggctgga attgggaaac gttcaacgtg ccggaaaggg
     1261 tcgcgctcgc cctcgcacgc ctcgggggaa aggtgctcta ttgcgagaat cccgtttccc
     1321 ggctgcgcgg ccgcggacgg cccttggaag agatcgaggg cggagtgcat cggtttggtc
     1381 ccgagttttt gggtcaccgc ttgaacctgg ttcctttcgg ttttccgcaa tttcaggcgc
     1441 gaatgatggc gaaccaaata ctccagaacg ccgcgaagct gaaactcaag gaccctcttt
     1501 ttctttacgc tcacggcgaa tttttcttgc cgctgtgccg cgaattcaaa cggaaaggtt
     1561 tttttctcgt tcatatctgc atggactatc ccgagcccgg ccaggagaaa cacattcaac
     1621 tgtcggacct gacgcttgta attcccaaaa gcgtgttcca ccaattaagg cccaaatacg
     1681 gagataaaat agctttgatt ccccaagtga ccgcgatgct ctcttcggat tgtcaggttg
     1741 gcgcaaatgc aagggcgtct gacgaatttt ccgccatacc cgggcctcgc cttggctaca
     1801 tcggcccagt aacagaccga ttgaacttga aggtcctgga aaaaatgctt agagctcgac
     1861 ctgactggca gttcattcac ttcggcgcgg cgaaatgtct tccccttccc aatgtgcatg
     1921 tgattgcgtg gcgcgacccg gggaaactga aagacgtgac cgcaaatctg gatgtgggtt
     1981 tcatgcccta cgactgctac agcaacaaaa attttcactg catgccgcta aaagtattcg
     2041 actactttca cgcaggtctt ccggttgtat ccacgtccat cgtgaattta tgggaattct
     2101 acgataccat ttattttggc gacgacgcgg aagaactctg ccgcgcagtc caatcggcgc
     2161 tcgacgagcc tgcggatagc ccgaaaaaat ccgcgcggat cgccatcgcc aaggagcatt
     2221 cgatcgaagg cctcgcggaa tgcctcgacc gcgtcctgtc ctctcgcggc ttgccggttc
     2281 gtcaaacgaa atgttaaagc ctgacacaag gactctccaa aatgagtgaa gaaaactccg
     2341 cgtgcgcttg ggagaagtga tcaatgtcac gagcaagaaa cttcgcggct ggactgttgt
     2401 tgcgctcgcg gattcgattt cggtcgttgc aaaaggtacc cgtgattggc ggatcgctcc
     2461 accgccttag ccattggcta ctaccgggtc agcttagggt gtggtcccgc gtccggcaag
     2521 ggccggcaaa ggatatttgg ctcgaggtga atccgaggac tggacaagga tacattcagg
     2581 ggaccacgga agcagctact cagcgtcgat tggcggaata tctgaagccg gggatggttt
     2641 tctacgatct gggcgcaaac attggcctgt tttcattatt ggcagcgcgc atcgtgggac
     2701 cgcagggcag agtatttagt tttgagccgg attttgagac agcgcagcgc cttaagagca
     2761 atgttgaaaa gaactcatgc tgcaacatta cgattgtcga ggcgggcgtg tggtcatcga
     2821 cccgcaaagt agccttcatt gcgtctgatt ctaccttgga tcgcggattc ggcaggttta
     2881 cgctcgaaac ggcaaacatc gcggggaaag aactcccgtg ttacgccctc gacgatttca
     2941 tgagcactgc tccaccgcca aatgccatta aatgtgacgt cgaaggcgcc gaactggaag
     3001 tatttcgcgg cggggaacac ctgattgcca cccatcggcc atggattatc tgcgaaattc
     3061 attcagcgga aaatggcgaa gcagttttga acctctttcg ccgatttgga tacagcgttg
     3121 acgccgtgga tagcaatcat cttcttgcgt ttccttagat ggttccgagg tagcctggag
     3181 tgttgaagaa gtttcgcgta ctgctgttgt gcacgcaccc ggttcaatac ggctcgccca
     3241 tgtggcgcct tatggcgcag cgtcccgagc tagaagtact ggtcgcttac tgcagcctgg
     3301 aaggcgccga ggcgcacgtt gaccccggat tcggcgtcga ggtcgtctgg gacgtacccc
     3361 ttctcgataa ctatccgtgg gtgcacctca agaatgtttc gccgcgtcca gcaatcggcg
     3421 gattctttgg gctgatcaac cccggcgtct ggcggctcat acgatctgga aaattcgatg
     3481 ctgtcgtcat gttcacgggc tacgtttgtg cgacgttttg gatcgccacc gccgcggcga
     3541 aactctctgg catcccgatt ctcttcggca cggacgccca tgatctaagc ccgcgcggcg
     3601 ctcgcgcctg gaaaacaaaa attaaaaaat atctttggcc ccgattgttt cgtctcgcgg
     3661 atatcgtcat cgcgccatcc tctggcactg tagcgttgat gcgttcgctg cgagtccagc
     3721 ctgaccgcgt gatgctaatg ccctatgtcg ttcacaacga atggtggatc gagcaggctg
     3781 cgcgtgtgga tcgtgccgcc gtgcgccgcg gctggaacat tcctgtcgag gcccccgtag
     3841 cacttttttg cgctaagttg cagccttgga aacgccctca ggacctgctg ctcgcctttg
     3901 ccagtgcaaa tgttcctggc agctatctcg tctatgcggg agatggtccg atgcgaagcg
     3961 aacttgaggc tcaagcgaaa actctcggca tttccgctca aactcgtttc ctcggattcg
     4021 tcaatcagtc cggtttgccg cgggtgtact gcggttccga tctgcttgtt ctgccttccg
     4081 aatatgagcc ttttggcgtc gta
//
LOCUS       DATNDT010000383         2742 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3359669, whole genome shotgun sequence.
ACCESSION   DATNDT010000383 DATNDT010000000
VERSION     DATNDT010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3359669"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..851)
                     /locus_tag="VN788_12450"
     CDS             complement(<1..851)
                     /locus_tag="VN788_12450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU21383.1"
                     /translation="MKALSTIGLMIGSLVLSGSGLWLWAQERENPTFRVKVDLVVLSF
                     TVTDNKGHYVNGLKPSDFRIFEDGIRQKMNTFGEGNKPPLQILADGSTRPLITAPAAP
                     GDGKTVDLRNDSFVGTNVFVLFDTSNYMYRGFVYASDAIADFIRGLDRADSVAVYTFS
                     RNLSRPAPLDRNRTNAIFGLRKAVAGDDAALYNGLLLTLRDAAKVPGRKVVIVFSNGP
                     DNASMVAPDDVRAVAEDEGIPIYVISTSEVNKDPISSAVFRRISNRTGGKAYFAKTWQ
                     KQIEAFE"
     gene            complement(871..>2742)
                     /locus_tag="VN788_12455"
     CDS             complement(871..>2742)
                     /locus_tag="VN788_12455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21384.1"
                     /translation="WKDPDDVLEALFGLCRKSVENEPLKIFMTLTDVDTHRAEPLSAA
                     TADRMARAYRQFGSQYAIFSEVPEVSNATINKFLDVAESTNQIHDQSLRADVTGSVQA
                     LTGLWQILYRQRSIPASEADSTLSNLLAPFSAVKDEQALFDNSRADVKLLLAAAHSPV
                     SALPQDRMFDLLAGNGSSENPQVQTQLVEEMMRIFEAQRLVSLNTLFNLADNLDAMSK
                     GEKANTALVDKMAARISEIQLPRETLSGMEKSSLSFGYWSEKHIDAQRKLNLRAIIDR
                     ASTDPKKIEGVRGLLAPFLRDTLVGLNYIYYAPPGAQILLTNPLFVRSHDFVGMQGIN
                     EAWRETEVQGSGWPTSAGGKLVGSLAGLPYALAEAEQNFLIPNREQALIWGDLVPQIL
                     VSATVQRWWRVSPAQMHWVALHMRLASALLAESCLNADRRREFLDELSLHAPPVRVAR
                     VRKDLEEAMPGQALEELMPSEMFTIARETIASGKLGNEPIAAEIRRIEKESPEEVNYA
                     VISREFGTPKPTLANSYAPQLLGMRTFPALMGYSSRILAESWESSLLYYATIADAMHL
                     QPAELNIMIPQWTEQTVEQIFATHLEDWPALLRSLRVVGQEALEKDHKQVASLRGSN"
BASE COUNT          543 a          842 c          762 g          595 t
ORIGIN      
        1 ctctcgaacg cttcgatttg tttctgccat gttttcgcaa agtaagcttt gccgcccgtg
       61 cgattcgaaa tacgtcggaa cactgcgctg gaaattgggt ccttgttcac ttcgctggtc
      121 gagatgacgt agatcggaat gccctcgtct tcggccacgg cccggacatc gtcgggagct
      181 accatgctcg cattgtccgg cccattcgaa aacacgatga cgactttccg ccccggcact
      241 ttcgcagcgt cgcgcaatgt cagcagcagg ccgttgtaca gcgccgcatc gtcgcccgcc
      301 acggctttgc gaaggccgaa gattgcattc gtccggtttc ggtccagagg cgcgggccgc
      361 gagagattcc ggctgaatgt gtaaactgcg acggaatcgg ctctgtccaa tccccgtatg
      421 aaatccgcga tcgcgtcgga ggcgtagacg aatccccggt acatgtaatt gctggtatcg
      481 aagagaacga acacgttggt tccgacaaac gagtcgtttc gcagatctac ggtttttccg
      541 tctccaggcg ccgcgggcgc cgtaatcagc ggtcgcgtcg aaccgtccgc cagtatctgc
      601 aacggaggct tattgccttc gccgaaggta ttcatcttct ggcgaatgcc atcttcaaaa
      661 atacgaaaat cgctcggctt caggccattc acgtaatgac ccttgttgtc ggtcaccgtg
      721 aagctgagca caacgagatc gaccttcacg cgaaaagtcg gattctctcg ctcctgcgcc
      781 caaagccaca gccccgatcc cgaaagaacc aggctgccaa tcatgagtcc gatcgtcgat
      841 aatgccttca ccgcgtccta cctccgggaa ctaattcgaa ccgcgcaggc tcgcaacctg
      901 cttatgatcc ttctcgagtg cctcttgccc caccacccgg agcgaccgca aaagcgccgg
      961 ccagtcctcc aggtgcgtgg cgaatatctg ttccaccgtt tgctcggtcc attgaggaat
     1021 catgatattg agttccgccg gctgaagatg catggcgtcg gcgatcgtgg cgtaataaag
     1081 caagctcgat tcccagctct cggccaggat ccggctcgaa tagcccatca gcgcggggaa
     1141 cgtccgcatt cccagcaact gtggcgcgta ggaattcgca agcgtcggct tcggcgtccc
     1201 gaactcgcgc gagatcaccg cgtagttcac ttcttccggc gactctttct cgatgcgccg
     1261 gatctcggcc gcgatcggct cgtttccgag cttgccggac gcaatcgttt cacgggcgat
     1321 tgtgaacatt tccgaaggca tcaattcttc aagcgcctgc cccggcatcg cttcttccag
     1381 atctttgcgc actctcgcca cgcggacagg cggcgcatgt aatgaaagtt catccagaaa
     1441 ttcgcgccgg cggtccgcat tcaggcagga ttccgcaagc agcgccgacg caagcctcat
     1501 gtgcagcgca acccaatgca tctgcgccgg cgatacgcgc caccaccgct gcacggtcgc
     1561 gctgaccaga atctggggta ccagatctcc ccagatcaga gcctgttcac ggttcgggat
     1621 caggaagttc tgctccgctt ccgcgagcgc gtacggcagg cccgcgagag atcccaccag
     1681 tttgccgcct gcgctggttg gccagcccga gccttgcact tccgtctcgc gccacgcttc
     1741 gttaatgccc tgcattccga cgaaatcgtg gctgcgcacg aacagaggat tcgtcaacag
     1801 gatctgcgcg ccgggagggg catagtagat gtaattgagt cccaccagcg tgtcgcgcag
     1861 aaacggcgca agcaggccgc gaactccttc gatcttcttt ggatctgtgg aagccctatc
     1921 gataatcgcg cggaggttca gctttcgttg cgcgtcgatg tgcttctccg accagtagcc
     1981 gaacgagagc gagctctttt ccatgccgct caacgtttcg cgcggaagct ggatttcgct
     2041 gatccgcgcg gccatcttgt ccaccagcgc cgtattggcc ttctcgccct tcgacatcgc
     2101 gtctagattg tcggccaaat tgaataacgt gttcagcgaa accaggcgtt gcgcctcgaa
     2161 aattcgcatc atctcttcga caagctgcgt ctggacctga ggattttcgc tactgccgtt
     2221 gccggccagc aggtcgaaca tccggtcttg cggaagagcg ctcacgggtg agtgcgccgc
     2281 cgcgagaaga agctttacgt ccgcgcggct gttgtcgaaa agcgcctgct catccttcac
     2341 cgccgaaaat ggagcgagta gattcgaaag cgtgctgtcg gcctcgctgg cggggatgga
     2401 acgctgccgg tataggatct gccacaagcc ggttagcgct tggaccgatc ccgttacgtc
     2461 ggcgcgcaac gactgatcgt gaatctgatt tgtagattca gctacgtcga gaaacttgtt
     2521 aatagtggcg ttgctgactt ccggaacctc gctgaaaata gcgtactgag acccaaattg
     2581 ccggtacgcc cgcgccatgc gatcggccgt ggcggcgctc aacggctccg cgcgatgtgt
     2641 gtccacatcg gttagcgtca tgaaaatctt gagcggttcg ttttccacgg acttgcggca
     2701 gaggccaaat aaagcttcga gaacgtcgtc ggggtctttc ca
//
LOCUS       DATNDT010000384         6866 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3490032, whole genome shotgun sequence.
ACCESSION   DATNDT010000384 DATNDT010000000
VERSION     DATNDT010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6866
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3490032"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2121
                     /locus_tag="VN788_12460"
     CDS             <1..2121
                     /locus_tag="VN788_12460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21385.1"
                     /translation="LLEDVWITIYGPGGKRDDNIHTRKCSYEPETGDVRCEGVVQIDI
                     GKARPGVSQGTASGESLEVTTSDLLFNRNTGEASTQAPVEFRFAGGNGRGVGIRYSTR
                     DSIVRVEHSVEFDLASSPVTEGLPITATGTSLELRRNQRTVVLDGPAVVRQGDREVSA
                     GTISVALDADYRAERVIVESNPQIRAKENGANEIASAGRFEASLSAAGRVERIIADGK
                     VFASRQTIMGSDRFSAAHVEFEMLPVGNLIKQMIASGGVEAESRKGSDSQNLKTAALL
                     VAFSAVSGHGSGRSNPTGDDPVGRQKIEMAETLAPATIESKTGADVITLRAKQFVAEA
                     SPDGRISKLFGHSGVDIRRQASHQNQEVSSARELVATFGARGEWDTLDETGDVHFSQA
                     GREATAAHAYVVRATDTATLEGSPAISDAMSRSTAGKVVFDQKTGEVNASGGVVSTYI
                     PSGAGDPVSLGSGAAHISADKLSGSASSGHVVYAGHARLWQGAAVLDSDQIELWRDTK
                     KMQAKGHVVAVFPQIGGPFAALPGSNVASRQASPSQPVLWKVRAPMLTYSADEGKAHL
                     EGGVETGSSQGSLESRTLDVFLGLATVASTQAPSLTGALPSTSPESRVATQMSEGGRQ
                     LSRVLAQGNVVVRQGDRRGKADQAEYTAADGKFVLSGGQPTLANALSDTTTGHSLTFF
                     VASDTILIDSQAGSRTLTKHRIEK"
     gene            2128..2853
                     /gene="lptB"
                     /locus_tag="VN788_12465"
     CDS             2128..2853
                     /gene="lptB"
                     /locus_tag="VN788_12465"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HXU21386.1"
                     /translation="MLKLQAAELRKSYRRRTVVNDVSLEVQQGEVVGLLGPNGAGKTT
                     TFYILVGLARPDYGRVLLDGEDITDLPMYLRARSGISYLPQEPSVFRKLTVEENLLAV
                     LETLSVTPEQRRDRVEELLAQMGLEGVRSSQAHVLSGGERRRLEIARSLVLSPSFVLL
                     DEPFAGIDPLTVVDIQKIIADLSEAGIGVLITDHNVRDTLAVTNRAYIISEGKILAAG
                     TPLELSANADVRRIYLGEGFRLN"
     gene            2858..4300
                     /gene="rpoN"
                     /locus_tag="VN788_12470"
     CDS             2858..4300
                     /gene="rpoN"
                     /locus_tag="VN788_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HXU21387.1"
                     /translation="MNWLAPKLHLRLAQKQILTPGLVQMVSVLALNRLELREMINQEI
                     MANPVLEELGEDGVTGDSYTDEAFLKKETEKVPETEAPNPFDEFDFGSFFTQYLDTGA
                     ERSAGGEHEEIERPSFDKFLSSPASLTDHLTWQMSASICTETVRKIAESIIGNLDENG
                     YLTATLDEIAQSGNYSMEDVEEALALVQELDPPGVGARNLEECLILQVKVLDPQNILA
                     QQIIGEHLKLLQNNQLKEVARALNRPVEMVKRAVDVIKRLDPKPGLRYNKIEPRLVEP
                     DVQFRKVDGEWQVFMNDDDVPQLRLSPTYRRLLARDAADRDVRNYVKERFTAAIQLMK
                     NIEQRKHTILRVCHSILRRQGDFLDYGPDHLRPMMIKEVAEEVGVHPSTVSRAVANKY
                     VHTPQGVLELRSFFSESVNGPQGGDMSLLSLKRRVKQMIEAEDSAHPLTDEQITKKLS
                     EEGIQVTRRTVAKYREDLRIPSTHRRRIKA"
     gene            4313..5200
                     /gene="rapZ"
                     /locus_tag="VN788_12475"
     CDS             4313..5200
                     /gene="rapZ"
                     /locus_tag="VN788_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HXU21388.1"
                     /translation="MKRADRKRHARQLVILTGLSGSGKGSVLNTFEDLGFYCVDNLPV
                     ALIPTFSELYEEGRGEVDRAALLVDAREGEQIEKLPGIYRRLTAEHATTLVFVEASDA
                     ALQRRFSETRRPHPLGRSGSIIDGIREERHRMAPIRRLADVVIDTTKFNVHELRQFVV
                     DRFQNPGRRPLLVSLVSFGFRYGIPADADLVFDVRFLPNPHFVPRLRPFDGRNSRVAR
                     YIHSFPQTAEFRRRMESLLTYLIPHYIREGKSYLTVAFGCTGGRHRSVALAEVTRRAL
                     QKKGYATKVVHRDLDKSTA"
     gene            5335..>6866
                     /locus_tag="VN788_12480"
     CDS             5335..>6866
                     /locus_tag="VN788_12480"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU21389.1"
                     /translation="MVSPGAEAKPLILPATARQEIARLLGYLRPYVPRLALGIVLIAA
                     MGAVDFLIAFAFRPAFDVILNPHSTAQALTLFQIPITHRAVDLHSFVPRHFHNVWTVF
                     AVALLLLTFIKGTAEYVGSILIQHVGLSGVTDLRNEVYSQVVQQPVGFFQHNPTGRVM
                     SAVISDIEQMRSAFSDWFAEFFRQLFALIAFVTVLLVINWRMALGSAILIPAVVWPVS
                     KFGRRIRRSSEKSRSRLADLSEILQETIAGNRVVKAFGMEGFEIRKFREAARHLLREN
                     MRWIRAFAATSPVMDILGAVVIALVLLVARGEIKAHRMTIGLFVSFAYALFKSYEPIK
                     RLGNVYQLFLQAAGTAMQVFTFLDLPKEVMDAPGAKVLPPFSRSIEFERASFAYETGP
                     PILKNIDLKVSAGAVVAIVGSSGAGKTTLVNLLPRFYAASSGSIRIDGYHLSDVTLRS
                     LREQMAIVTQETILFNDTVRNNLCYGRPDMPDDKIIAAAQAALAHDFIIRMPQGYQTV
                     IG"
BASE COUNT         1481 a         1900 c         2022 g         1463 t
ORIGIN      
        1 ctattagaag atgtgtggat cacgatttat ggccccggcg gcaagcgcga cgacaacatc
       61 cacacccgca aatgcagcta cgagccggaa accggggacg tgcgctgtga gggcgtggtg
      121 cagatcgaca ttggaaaggc acggccgggt gttagccaag gcaccgcgtc cggcgaaagc
      181 ctcgaagtaa caaccagcga tctcctgttc aatcggaata cgggcgaagc ctcaacgcag
      241 gcgccggtcg agttccgctt tgcaggtggg aatgggcgag gcgttggcat tcggtatagc
      301 actcgggatt cgattgtgcg cgtggagcac tccgtcgaat ttgacctggc gtcgtccccc
      361 gtgacggagg gcttacccat aaccgctacg ggcacgagcc tcgaattgcg acgcaaccag
      421 cgaaccgtgg tgctggatgg gccggcggtt gtgcggcaag gcgatcggga agtttcggca
      481 ggcacaattt cggtggcgct tgatgcggat tatcgcgccg agcgcgtgat cgtcgagagc
      541 aacccgcaaa ttcgcgcgaa agagaacggc gcgaacgaaa tcgcgtccgc tgggcgtttc
      601 gaagcctcac tgagtgccgc gggccgcgtc gaacggatca tcgctgacgg caaagtattc
      661 gcgagccggc aaactatcat gggttccgat cgtttttcag cagcccatgt cgaattcgag
      721 atgcttccag ttggtaactt gatcaagcag atgatcgcca gcggcggcgt tgaggcggaa
      781 tctcgcaaag gcagtgattc gcaaaacttg aaaaccgccg ccttgcttgt ggctttctcc
      841 gctgtgagcg ggcatggttc aggaaggtcg aatcccacgg gcgatgaccc ggtgggtcgt
      901 cagaagatcg aaatggccga aactcttgct ccggcgacaa tcgaatcaaa aacgggcgcg
      961 gatgtgatca ccttgcgcgc gaagcaattt gtggcggagg ccagtcccga tggacgtatc
     1021 agcaaattat tcggacactc cggcgtagat attcgcagac aagccagcca ccaaaatcaa
     1081 gaagtgagct cggcgcgaga gcttgtggcg acttttgggg cgcgtgggga gtgggacacg
     1141 ctcgacgaga caggggatgt ccatttcagc caggcagggc gtgaagcgac cgccgcgcac
     1201 gcctacgtcg tccgcgcgac cgatacggcg actctggagg gctcgcccgc tatttctgac
     1261 gctatgagcc gctcgacagc aggcaaggtc gtgttcgacc agaagacggg cgaagtgaac
     1321 gccagtggag gagtggtctc gacttatatc ccgagcgggg caggagaccc ggtgagcctg
     1381 ggttctggcg ccgcccacat ctcggccgat aaactttcag gctcggcaag ctcgggtcac
     1441 gttgtctacg ccggccatgc tcggctatgg caaggagccg ctgtgctgga tagcgaccag
     1501 attgaacttt ggcgcgatac caagaaaatg caggccaagg gacacgttgt cgcagttttt
     1561 ccgcagattg gcggaccatt tgccgcacta ccgggaagca acgtcgcgtc ccgacaagct
     1621 tcgccgtcgc aaccggtctt gtggaaagtt cgggctccga tgctcacgta ttcggctgat
     1681 gagggcaaag cccatcttga agggggtgta gagaccggtt cgagccaagg ctccctcgag
     1741 tcccgcactc tggacgtgtt tctggggctg gcgacggtcg cttcgacaca ggcaccttcc
     1801 ctaactggtg cgttaccttc gacttcgcct gaaagtcggg tagcaacgca gatgtccgaa
     1861 ggtggacgcc aactgagtcg agtgctagcg caaggcaatg ttgtggtccg ccagggagat
     1921 cggcgaggga aggccgatca ggccgaatac acagctgccg acggaaaatt cgtcctttcc
     1981 ggcggtcagc cgacgctggc taatgctctt agcgacacaa ctacgggcca ttcgttgacc
     2041 ttcttcgtag cgagtgatac gattttaatt gattcgcaag caggttcgcg cacactaacg
     2101 aagcaccgga ttgagaaatg aaacggcatg ttgaaactcc aggccgccga gctccggaag
     2161 tcatatcgcc gccggactgt cgttaacgac gttagcttgg aggttcaaca gggagaagtg
     2221 gttgggctac tcgggccgaa cggcgcggga aagaccacga cgttttatat cctagttgga
     2281 cttgctcgcc ccgattacgg gcgtgtcttg ctggatggcg aggatatcac cgacctcccc
     2341 atgtacctgc gggcccgcag cggtatcagc taccttcctc aggaaccctc tgtctttcgg
     2401 aaactgactg tggaggagaa tttgctagcg gtgttagaaa ccctgtccgt gaccccagaa
     2461 cagaggcgcg accgggtgga ggagctcttg gcacaaatgg gactagaagg agtgcgctcc
     2521 agccaggccc acgtgctctc gggtggcgag cggcggcgtt tggaaatcgc gcgctccctg
     2581 gtgctctctc cgtccttcgt tcttttggat gagccttttg cgggtattga tccgttgacc
     2641 gtggtggata ttcaaaagat catcgcagac ttaagcgaag ctggaattgg cgttctgatt
     2701 actgatcaca acgtgcgcga tacgctagcc gtgaccaacc gcgcttacat cattagcgag
     2761 ggtaaaattc tggctgcggg cacacccttg gaactttccg cgaatgccga tgttcgccgc
     2821 atctacttag gagagggttt ccggctcaac tgaacctatg aactggctcg cacccaaact
     2881 gcacctccga ctggctcaga aacagattct gacgcctggc ttggtgcaaa tggtcagtgt
     2941 actggccctg aatcgcctcg agcttcgaga aatgatcaat caggaaatta tggctaaccc
     3001 cgttctcgag gaactcggtg aggacggcgt taccggcgat agttacaccg acgaagcatt
     3061 tctcaaaaaa gaaacggaaa aggttcctga gacagaggcc cccaatccct tcgatgagtt
     3121 cgatttcgga tcgtttttca cccaatatct ggatactgga gccgagcggt ccgccggcgg
     3181 cgagcatgaa gaaatcgaaa gaccttcgtt tgataagttt ctctcgtcgc ccgcaagtct
     3241 taccgaccac ctgacatggc aaatgagcgc cagtatttgc actgagactg ttcgcaagat
     3301 tgccgaatcg atcatcggca acctggacga aaacgggtac ttgacggcga cactggatga
     3361 aatcgcgcag tctggaaact attcgatgga agatgtcgag gaagctctgg cattagtcca
     3421 ggaactggat ccccccggag ttggcgcgcg caatttggaa gagtgcttga tccttcaggt
     3481 aaaggtgctg gatccccaaa atatcctggc ccagcagatc atcggcgagc atctgaaact
     3541 cctacagaat aaccaactca aagaagtcgc gcgcgcgctg aaccggccgg tggaaatggt
     3601 gaaacgagcg gtggacgtca tcaagcgtct cgatcccaag ccgggcctcc gctacaataa
     3661 aatcgagcca aggcttgtcg agccggatgt tcaattccgc aaagtcgacg gtgagtggca
     3721 ggttttcatg aatgatgatg atgtgccgca gctgcgctta agtcccacgt atcgccgact
     3781 gctggcgcgc gatgcggcag atcgagatgt ccgaaactat gttaaggaac gttttacggc
     3841 ggccatacag ttgatgaaaa atatcgagca gcgcaagcac accatcctgc gcgtctgcca
     3901 ctcaatcctc cgccgccagg gagatttctt ggattatggc cccgatcatc tgagaccgat
     3961 gatgattaag gaagtcgccg aagaagtcgg cgtgcatccc tctacagtga gccgcgcggt
     4021 agccaacaag tacgtgcata cgccgcaggg cgttttggag ttgcgaagtt tcttctccga
     4081 atcggtcaac ggcccgcaag gcggcgacat gtcgctccta tctctcaagc gccgcgtgaa
     4141 acaaatgatc gaagcggaag acagcgcgca tcccctcacg gatgaacaga ttacaaaaaa
     4201 actcagcgag gaaggcatcc aggtcactcg gcgaaccgtg gccaaatacc gcgaagattt
     4261 gcgcatcccg agcacccacc gccggcggat aaaggcttaa gcggcgaccg ccgtgaagcg
     4321 cgccgatcgg aaacgtcacg cacgccagct cgtaattctt acaggtcttt cggggtccgg
     4381 caaaggttca gttctcaata cgtttgaaga cctcggcttc tactgtgtcg ataatcttcc
     4441 tgtagccctc attccaactt tcagcgagct ttacgaggaa ggccggggcg aagtggatcg
     4501 tgctgcgctc ctggtcgatg cgcgcgaggg tgagcagatc gaaaagctac ccggcatata
     4561 tcgcaggttg accgccgaac atgccactac gctggtcttc gttgaagcga gcgatgcagc
     4621 gctccagcgc cgttttagcg agacacgccg tccccacccg ctcgggcgga gcggttcgat
     4681 catcgacgga atccgcgaag agcgccaccg catggctccg atccgccgtc tcgcggatgt
     4741 cgttatcgac acgactaagt tcaacgtgca cgagctgcgc cagttcgttg tcgatcgctt
     4801 tcagaatccc ggccgccggc cactgttggt atcgctcgtg agcttcgggt ttcgttacgg
     4861 cattcccgcg gacgccgatc tggttttcga tgtgcgcttt cttccgaacc cgcatttcgt
     4921 gccgcgactg cgtcctttcg atggaagaaa ctcgcgagtg gcgcgctata tccattcttt
     4981 tccgcagacg gcagagttcc ggcggaggat ggagagcctg ctgacgtact tgatcccgca
     5041 ctatatcaga gagggcaaga gctatttgac ggtcgccttc ggatgtacgg gagggcggca
     5101 ccgctccgtc gctctggccg aagtgacacg ccgcgcgctt cagaagaaag gatacgccac
     5161 gaaagtggtg caccgagatc tcgataaatc caccgcgtga actcatggag accggcggat
     5221 tgcatggcgg tagccagaca gcagccgacg cgattacggc ttgcaagatg cttggacgat
     5281 gcataggcta acaggctcgg cacggtatcg cgactctacg ataggaaaat ggatatggtg
     5341 agtccggggg ctgaagcgaa gccacttatc ctgcccgcga ctgcgcgcca ggaaatcgct
     5401 cgcctgctgg gttatctgcg cccctatgtg ccgcgcctcg ccttgggtat tgtcctgatc
     5461 gccgcgatgg gagcggttga cttcctcatt gccttcgcct tccgtcccgc cttcgacgtg
     5521 atattgaacc cgcattcaac ggcacaggcg ctgaccttat ttcaaatacc gattacgcac
     5581 cgagcagttg atctgcattc attcgttccc cgccattttc acaatgtgtg gacggttttt
     5641 gcggttgccc tcctactgct cacattcatt aagggcacgg cggagtatgt gggaagcatc
     5701 ctgatccagc acgtggggct ctcgggcgtt actgacttgc gcaatgaggt atatagccag
     5761 gtggtccagc agccggtggg tttctttcag cacaatccta ctggtcgtgt gatgtccgcc
     5821 gtcatcagcg atatcgagca aatgcggagc gccttctccg attggttcgc ggaatttttt
     5881 cgccagctgt ttgctctgat cgctttcgtc accgttctac tcgttatcaa ttggagaatg
     5941 gccctggggt ctgcgatttt aattccggcg gtggtttggc cggttagcaa attcggacgc
     6001 cgcattcgcc gctcctcgga aaagagccgt tcgcgcctgg cggacttaag cgagatcctt
     6061 caagagacga tcgccggaaa tcgcgtggtg aaggcgttcg gaatggaagg ttttgagatc
     6121 cgcaaattcc gcgaggccgc gcgccatctg ctccgcgaga atatgcgctg gattagggcg
     6181 ttcgccgcca cctcgccggt aatggacatt ttgggagcag tcgttatcgc gctggtgctg
     6241 ctcgttgcgc gcggcgagat caaagctcac cgcatgacga ttggattatt cgtttctttc
     6301 gcgtacgcgc tcttcaagtc ttatgagccg atcaagcgcc ttgggaacgt ctatcagctg
     6361 ttcttgcagg ccgctgggac tgccatgcaa gtattcacct tcctcgactt gcccaaagaa
     6421 gtgatggatg ctccgggcgc aaaagtactg ccgccattct ccaggtcgat cgaatttgag
     6481 cgcgcgagtt tcgcttatga gaccgggccg ccgattttga aaaacatcga cctgaaggtt
     6541 tccgcgggcg ctgtggtcgc catcgttgga tcgagcggcg ctggaaaaac gacccttgtt
     6601 aatcttcttc cacgttttta cgcggcgagt tcaggctcga tccggatcga tggctaccat
     6661 ctttcggacg tgacccttcg ctcgttacgc gagcagatgg ccatcgtcac tcaggaaact
     6721 attctcttca atgacaccgt acggaacaat ctctgttatg gtcgcccaga tatgcctgat
     6781 gacaaaatca tcgctgcagc tcaggcggct ctggcccacg atttcatcat ccgcatgccc
     6841 cagggctacc aaacggtgat tggtga
//
LOCUS       DATNDT010000385         6740 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1237277, whole genome shotgun sequence.
ACCESSION   DATNDT010000385 DATNDT010000000
VERSION     DATNDT010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6740)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6740)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6740
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237277"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /locus_tag="VN788_12485"
     CDS             complement(<1..528)
                     /locus_tag="VN788_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HXU21390.1"
                     /translation="MTYAIVLVTVVGVLCGVAIFKTIGVRNRADFLVAGRSLPWPVLV
                     FTLLSSWIGAGSLFAGGENAYKNGFAALWQPAGGWLGLIVIAMIAGKVRRFAQFTVPD
                     LLETRYNSAARVLATIAIVISNTVIASYQFKGGGDILHLIFPSLDRNTGMYIIAAFVI
                     VFTAGAGMASIAYLDL"
     gene            complement(525..1712)
                     /locus_tag="VN788_12490"
     CDS             complement(525..1712)
                     /locus_tag="VN788_12490"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU21391.1"
                     /translation="MRSLLPFQEPRPESEFWALKNVSFRVEKGEVFAIMGPNGCGKST
                     LLQIISGILEPTSGRVLRSGRVAALLELGAGFNPEFTGRENVYLNGEIMGLSRAEIER
                     AFPKIENFAEIGKFIERPVKEYSSGMYVRLAFSTAIHVDPEILIVDEALAVGDAVFAA
                     RCVRKFEELRERKMTVLLVSHDLGLVKRLADRAVFMLNGEVRIEGSPKDAVNRYVGFV
                     LEREGTRNPEEAAANALSSSFRHGDGASRVIDVKILNGAGQECYAFQRGEAMRIRIRA
                     LFYQTVQSPVIGILIRNRLGMDVFGTNSRIEQTDFGEVLAGQELEAEFALDCLLTRQE
                     YTLTVAAQYWNGLSQDWLDDTVSFRVVDAKDVAGVLHLETQVTYHIQPRVAIEEGKAL
                     PHA"
     gene            complement(1804..4548)
                     /locus_tag="VN788_12495"
     CDS             complement(1804..4548)
                     /locus_tag="VN788_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21392.1"
                     /translation="MADTLEKLRPDRDLQCYFFIPSAVAALSGASENAFTLSGSFRQQ
                     FDWAVVEWNRDNTFEHPAFRYLPDGDLSGLTLSYQETRTNCVPMDSDINPTVAWPSLR
                     IWADAGSGEQIYFVPLLKYASAVAGIYQSATVELTLQGTVTAGDYVGFSFLDEHYTYQ
                     YFGGDALEVAIQQLAAAVKAFSPTMDAAQINGTTIQLHFKKTGLNGNRIGVYTYVSGG
                     GTEQWDAPVKQFSDGTSPTTWQVTIPFGGLDELPLVAAKNIRKMRWTYAADIQNGAYQ
                     RSEFQVTITNWTVTGVGREYSVAGPGSRRIEDDDPVVQYTGNWTLWQDNFSGGSIHYV
                     TGGPASLSCKYYSPQMHSLYLGVLRTSSSGTVQVSVDGGAPTPLSVSVNDDNVLARLP
                     LGEFGPGDHTVSVTNGGAYFYFDFLEIAIPRTELPVFDTEPKFTLATDWDTYQSLSIA
                     PERTAWMINSLGFKGRVNHYVGALWFYELVPQGQQYASATVTFTGSVDTTHATTSLVI
                     GEVGGGPSTQTTITHLNLIGDTPETLATAFALLINGGYTAIRAEATGNQLTIFARAIG
                     KAGEQVSLVASTTGPLLTVTTSGSALSGGSDGNSSANGTNWFTDLSAIPRINRAARDW
                     HSSYFQALKGYGIDVTASFSMELGNGDPSVAAGIAQRHSTGEPVVLNTPSLQTNFSPA
                     SMAFWQQVYADMAAIQNGAGVVPYLQFGEVQWWYFPYNIVGQDPLGMPFYDAYTTSAF
                     QAKYGYPLPVIATNGVDPSTVADAAAFLPGLIGAFTQAVMSYVLASYPNCRFEVLYPT
                     DVNDTPLNQVINYPTAIWTPQNLTCLKTESFSYTFERNLDMAARTIATGVALGFLPSQ
                     RSHLVGLEMASTAWLKEARLAEANAMDSVVLFALDQFCLIGYSIPLGHGMRRSSQFGQ
                     "
     gene            complement(4557..>6740)
                     /locus_tag="VN788_12500"
     CDS             complement(4557..>6740)
                     /locus_tag="VN788_12500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21393.1"
                     /translation="QEVSTTLTALGLPNFDQAGRAMSLQLNKSVAGNTYAEFETSVKG
                     VKLRPGDIITLTYAKEGWTRQPFRIVRIQPGPSYRTSVITAEIHDDAWYTTEANLTLT
                     GGRRIAAFEVGLPRPLTGTETDAEGHTEFGVAETAVESADGSFDVSLSIAFSTPQNTE
                     ASAALIPLVGLNATFAATGGTLTGAATYYYGISAVDETGTESALSFIITASVPAGTNT
                     NTVTLNSLSFSPGTAGVNVYRGVTPAQLLRIASNQPVAAQFTDTGFAATPAGPPDPNY
                     DHANFYWRFELQPEYPADIFSTITIGNSSLQMVVNQYQSAIVRITDGAGFGQERPIAT
                     NTATTLTVLTKWDIVPDATSKFVVAESSWHSGSTGSSSPVAFQVPNRPGMTVHVSGRA
                     ANVRNEETAYELSPLTPWQIGGATGSGMDADVPGQPSFALGLTGRGTVSVGAVGFADL
                     TNTRTIAAGTLALSYWDELANPSGFQLASGISASDTTITLNTAASAQINDLVQIESEV
                     MIVQAALNGGLGYQVTRGAYGTTGVAHNAPVLVYPLQKKTFVLPFPRGFFGSPASGSY
                     TFSLPLPDVRIATAELFVTNSQGNSDVNRQGFTATTDQGLRTLSGGQLSIQVEGLLAI
                     QTDAAPPLIMDAAHSIRDIFATVGTAATAGASIQLIITQNGQPYCPLTIPDPANPANL
                     TTSNIVSGFALGPLQAGALIGLDITSVTTNANELPGRDLTVTIRL"
BASE COUNT         1472 a         2078 c         1747 g         1443 t
ORIGIN      
        1 aagatcgaga taagcaattg atgccatacc cgcgccggcc gtgaacacga tcacaaacgc
       61 cgcgatgatg tacatgccgg tgttgcgatc aagcgagggg aagatcaggt gcagaatatc
      121 gccaccgccc ttaaattgat aactggcgat gacagtgttt gaaataacga tggcgatagt
      181 cgccagcacg cgcgcagcgc tgttataccg cgtttccaga agatcgggca cggtgaactg
      241 cgcgaaacgc cgcacctttc ccgcaatcat ggcgatgacg atcaacccta gccatccacc
      301 ggctggttgc cacagcgccg cgaatccatt tttgtaggcg ttctcaccgc ccgcaaacag
      361 gctgcctgct ccgatccagg aagatagcag cgtgaaaacc aaaactggcc atggcaaact
      421 tctgccggcg accaggaagt ccgcgcgatt ccggacgcca attgtcttga aaatcgcaac
      481 accgcagagc acgcctacca cggttaccag aacgatcgcg taagtcatgc gtgcgggaga
      541 gctttccctt cttcgattgc gacgcggggc tgaatatggt acgtgacctg tgtctccaag
      601 tgcaatactc cagcaacatc cttggcatcc acaacccgga aagagaccgt gtcgtcgagc
      661 cagtcctggc tcaagccatt ccaatattgt gcggcgacgg tcagcgtata ctcctgcctc
      721 gtcagcaaac aatccagggc aaactcagcc tcgagctctt gacctgccag cacctctccg
      781 aaatcggttt gttctatgcg gctgtttgtt ccgaagacgt ccatgccgag cctgttgcga
      841 atcagaatgc caattaccgg actctgaaca gtctgataaa acaaggcccg aatgcggatc
      901 ctcatcgcct cgccgcgctg aaatgcgtag cactcctgcc cggccccgtt caggatcttc
      961 acatcgatca cacggcttgc gccatcgcca tgacggaaac tgctgctcag cgcgttcgcc
     1021 gccgcctctt ccgggttgcg cgtcccttcc cgctccagta cgaacccgac gtagcgattc
     1081 acagcgtcct tcggcgatcc ctctatccgc acctcgccat tcagcatgaa gacggcgcga
     1141 tccgcaagcc gcttcaccaa cccaagatcg tggctcacca gcagcaccgt catcttgcgt
     1201 tcccgaagtt cttcaaactt gcggacgcag cgcgccgcaa agacagcatc gccaaccgcg
     1261 agcgcttcat ctacaatcag aatctccggg tcgacgtgaa tagccgtgga aaaagcgagc
     1321 cgcacataca tgccgctcga atactctttc accggacgct cgatgaactt accgatctcc
     1381 gcgaaatttt caatctttgg gaacgcgcgc tcgatctcgg cgcgcgacaa gcccatgatt
     1441 tcgccattaa gataaacgtt ctcgcggccc gtaaattccg gattgaagcc cgcgcccaat
     1501 tcgagcagcg cggccactcg cccactccgc aaaacgcgcc cgctcgtagg ctccagaata
     1561 ccgctgataa tttgaagcag agtgctcttg ccgcagccgt tcggccccat gatggcgaat
     1621 acttcgccct tctccacgcg gaacgagacg ttcttcagcg cccagaattc tgattccggc
     1681 cgcggctctt gaaagggaag caagctgcgc acacgcccag cgggactctt ataaaggaag
     1741 taccgcttcg aaacattttg gacggatatc acggccgcaa acgttcacta tagcccacct
     1801 tcattattgg ccgaactggc tgctgcgacg catcccgtgc ccgagaggaa tcgaatagcc
     1861 gatcaggcag aactgatcta gcgcgaaaag tacgacagag tccatggcgt tggcttccgc
     1921 aagcctcgcc tccttcaacc atgccgtcga agccatttca agtccgacaa gatgactgcg
     1981 ttgggacggc agaaaaccga gcgccacacc tgtcgcgatc gtccgcgccg ccatatccag
     2041 gttacgctcg aacgtataac tgaagctttc cgtcttgaga caggtgaggt tctgcggcgt
     2101 ccaaatggct gtgggatagt taatgacttg gttgagcgga gtgtcgttaa catccgtcgg
     2161 atacaacacc tcgaaccggc aattcggata cgaagcgagc acataactca tgacggcttg
     2221 agtaaacgcc ccaattagtc ccggcaggaa ggcagcagca tcggctaccg tcgaaggatc
     2281 tacaccgtta gtggcaatca ccggcaacgg atagccatac ttcgcctgaa aagcgctcgt
     2341 tgtgtatgcg tcgtaaaagg gcatccccaa agggtcctgc ccgactatgt tgtagggaaa
     2401 gtaccaccat tgtacctcgc cgaattggag ataaggcaca acacccgcgc cgttctggat
     2461 cgcggccata tccgcgtaaa cctgttgcca aaaggccatg cttgcaggcg aaaagttcgt
     2521 ctgcagcgac ggcgtattca acactaccgg ttcaccagtt gaatgtctct gcgcgatacc
     2581 cgccgcaacg gacggatcgc cattgcccaa ttccatgctg aaggatgcag taacatcaat
     2641 tccgtatccc ttcagcgctt ggaaatacga cgaatgccag tcccgcgccg cgcgattgat
     2701 tctcggaatc gcgctgagat ccgtaaacca gttggtcccg ttagcgctcg aattcccgtc
     2761 cgagccgcca gatagtgcgc tgccagaggt cgtgaccgtc agcaatggcc cggtcgtact
     2821 cgccacaagc gatacctgtt cgccagcctt tccaatcgcg cgcgcgaaga tagttagttg
     2881 gtttcccgtc gcctcggcgc gaattgccgt ataccctccg ttgatcagca atgcgaacgc
     2941 cgtcgccaga gtctctggcg tatctccaat tagattcaaa tgcgtaatcg tcgtctgcgt
     3001 agatgggccg cccccaacct ctccgatcac gagactcgta gtggcgtggg tcgtgtccac
     3061 gctgcccgtg aacgtgacgg ttgcggacgc atactgctgc ccttgcggaa caagttcgta
     3121 aaaccacaac gcacccacgt aatgattcac gcggcctttg aatcccaggg agttgatcat
     3181 ccacgccgtt ctctcaggcg ctatagacag cgattgatac gtgtcccaat cggtcgcgag
     3241 cgtgaatttt ggctccgtat cgaacaccgg aagctctgtc ctcggaatgg cgatttctag
     3301 aaaatcgaag tagaaatagg cccccccgtt cgtcacgctg accgtgtgat cccccggacc
     3361 gaactctccc aagggtaatc gcgccagcac attgtcgtca ttcacggaaa cactcagcgg
     3421 cgtgggcgct cctccgtcca cggacacttg aaccgtcccg ctgctgcttg ttcgcaaaac
     3481 gccgagatat aacgagtgca tttgcggcga atagtactta caactgagcg atgctgggcc
     3541 gcccgtaaca tagtgaatcg acccgccaga aaagttatcc tgccacagtg tccaattgcc
     3601 ggtgtattgg acaaccggat cgtcatcctc gatccggcgg cttcccggtc cggcgactga
     3661 gtactcacgt ccgacgccgg tcaccgtcca atttgtgatc gtcacttgga actcgcttcg
     3721 ttggtaagcg ccgttctgta tgtcggccgc atacgtccac cgcattttgc ggatgttctt
     3781 cgccgccact agaggaagct catcgagccc gccaaacgga atcgtgactt gccacgtcgt
     3841 cggcgacgtt ccgtccgaga attgctttac cggcgcatcc cactgctccg ttccgccgcc
     3901 agacacatac gtatacacgc cgatacggtt tccattgagt cccgtttttt tgaagtgcaa
     3961 ttggatcgtc gtgccgttga tctgcgccgc gtccatcgtt ggggagaacg ccttgaccgc
     4021 cgctgcaagt tgctgaatcg ccacttcaag cgcatcgccg ccgaaatatt gataagtata
     4081 atgttcatca aggaaactaa acccgacgta atcgccagca gtaaccgtcc cttgcagcgt
     4141 gagttctacc gtcgcagact gatagattcc cgccacagcg ctcgcatatt tcagtagcgg
     4201 cacaaagtaa atctgctccc cactgcccgc gtcagcccaa attctcaaag aaggccacgc
     4261 cacggtcgga ttgatgtccg aatccatcgg cacgcaattc gtccgcgtct cctgataact
     4321 gagagtcagt ccactcaaat ccccgtctgg aaggtagcgg aatgcgggat gctcgaaggt
     4381 attgtctcga ttccattcga ccacggccca atcaaactgc tgccggaatg agcccgacaa
     4441 agtgaaggcg ttttcgctcg ccccactgag cgccgccacc gccgatggaa tgaagaagta
     4501 gcactgcaaa tctctgtcgg gacgtaattt ttcgagagtg tctgccatga tgggccctac
     4561 aaccggatcg tgactgtcag atcacggcct ggcagttcgt tggcattcgt ggttactgaa
     4621 gtgatatcca ggccaatcaa agctcccgcc tgcagcggac ccaacgcaaa gccggacacg
     4681 atatttgaag ttgtgagatt agccggatta gcgggatcgg ggatcgtgag cgggcagtaa
     4741 ggctgcccgt tttgcgtaat tatcaattgg atcgaagctc cggctgttgc cgctgttccc
     4801 acggttgcaa atatgtcgcg gatgctgtgt gctgcatcca taatcagcgg cggcgccgcg
     4861 tctgtttgta tcgccagtaa tccctctacc tggatcgata actgcccgcc agagagcgtc
     4921 cgcaatcctt gatccgttgt ggcggtgaac ccctgccggt ttacatcgct gtttccttgc
     4981 gagttagtga caaacaactc cgccgtcgca atgcgcacgt ccggcagcgg aagcgaaaat
     5041 gtgtacgaac cactcgccgg actgccgaag aagcccctcg ggaacggcag cacgaacgtt
     5101 ttcttctgga gcggatagac gagcaccggc gcgttatgag ccacccccgt ggtcccgtaa
     5161 gcgccacgcg tcacctgata gcctagtcct ccgttcagtg ccgcttgtac aatcatgacc
     5221 tcggattcaa tctgaaccag atcgtttatc tgcgcgctcg cggccgtatt caatgtgatc
     5281 gtagtgtcgc tcgcacttat tcctgaggca agttggaacc cgctcggatt agccagttca
     5341 tcccagtaac taagtgccag cgtgcctgcc gcgatcgtcc gggtattcgt caaatccgcg
     5401 aaaccaacgg ccccaacact caccgttccc cgccccgtca gccctagcgc gaatgatggc
     5461 tgacccggaa catcggcatc catcccggag ccggtagccc ctccgatttg ccacggcgtg
     5521 agcggtgata gttcgtaggc cgtctcctcg ttacgcacgt tcgccgcccg tcccgacaca
     5581 tgcaccgtca tgccggggcg attcggcacc tggaacgcga ccgggcttga actcccggtc
     5641 gatcctgagt gccaacttga ttccgcaaca acgaacttac tcgttgcatc cggcacgatg
     5701 tcccacttag tcaaaaccgt cagggtagtg gccgtatttg tcgcgatggg ccgttcctga
     5761 ccgaatcccg caccatccgt gatacgtact attgcggatt ggtactggtt aaccaccatc
     5821 tgcaaactgc tgtttccgat ggttatcgtg gagaagatat ccgcgggata ttcgggctgc
     5881 aattcgaatc tccaatagaa gttcgcgtga tcgtaattag gatctggcgg gcctgccggg
     5941 gttgccgcaa atcccgtgtc cgtaaactgc gcggcaaccg gctgattgga tgcgatccgc
     6001 agtaactgcg ccggtgtaac tccccgataa acattcactc ccgcagtgcc gggcgaaaag
     6061 ctaagcgagt tcagcgtcac cgtattggtg ttcgtcccgg ctggaacgct cgccgtgatg
     6121 atgaacgaaa gcgcgctttc cgtccctgtt tcatccacgg cgctgatgcc gtagtaatac
     6181 gtcgcagcgc ccgtaagcgt accaccggtt gcggcaaatg ttgcgtttaa tccaaccagc
     6241 gggatcaacg ccgccgaagc ttccgtgttc tgcggtgtgg aaaatgcgat cgacaggcta
     6301 acatcgaaac tcccgtccgc gctctccaca gcagtttctg caactccaaa ctcagtgtgg
     6361 ccctccgcgt ctgtttctgt tccggtcagc ggcctcggaa gcccgacctc gaacgcagcg
     6421 attcttcttc cacccgttag tgtgagattt gcctcggtcg tgtaccacgc gtcgtcgtga
     6481 atttccgccg taatcaccga agtccggtaa ctcggccccg gctggatacg cacaatccga
     6541 aatggctgcc gtgtccagcc ttcctttgcg taggtgagtg tgatgatatc gccaggccgc
     6601 agttttacac ccttcacgct cgtctcgaat tcggcgtacg tgttaccggc taccgactta
     6661 ttcaactgca gcgacatcgc tcgtccggcc tggtcgaaat tcggaagccc gagtgccgtc
     6721 agtgtcgtgc tgacctcctg
//
LOCUS       DATNDT010000386        16305 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2173467, whole genome shotgun sequence.
ACCESSION   DATNDT010000386 DATNDT010000000
VERSION     DATNDT010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16305
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2173467"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VN788_12505"
     CDS             complement(<1..1037)
                     /locus_tag="VN788_12505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21394.1"
                     /translation="MQSTEFPFGVSVVICCHNSAARLPATLAHLVAQKVSKNIPWEVL
                     IIDNASTDGTATVARECWGEYHFAPLRIVWEQRPGTAFARQRGIAEARYELVSFVDDD
                     NWVTDQWVELTASFLRSHADVGALGGLSTPVFEVSPPSWFPACKAQYAISPETWLGGE
                     CTETRSLWTAGMTLRKSAWQQLEFKNIPILVPGRSGGSLTAGEDTELCYRLRLAGWKL
                     WYEPRLQFQHYMPKDRLNWAYARRLYRGAGKESAALLPYETVLHTEKSEAYSIIKQSW
                     EWKLLATAKGLLLRPRVLMRVATGNCEGDLEMLYLEVDIGRFTALARGRSSFYASINQ
                     VKKLAERLHSRG"
     gene            complement(1333..2067)
                     /locus_tag="VN788_12510"
     CDS             complement(1333..2067)
                     /locus_tag="VN788_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU21395.1"
                     /translation="MQEADCLAGHVAVVTGGSSGIGRAIALGLSRQGAQVCVVGRNPV
                     TLAETVAAAGQFSHIRGFQIDLTAEENLQPLLTHLKGEAGRLDILVHSAGLLRHDPME
                     RARVEDLDIQYNVNVRAPYVLTQHLLPLLTAARGQIVFINSSLALTAKRPEVGQYSAT
                     KHALKAVADSLREEVNGRGIRVLTVCLGRTATPMQEVLHRQEGKNYRPEMLLQPDDIA
                     SVVVQALTLPSTAEITDISMRPMRMT"
     gene            complement(2078..3166)
                     /locus_tag="VN788_12515"
     CDS             complement(2078..3166)
                     /locus_tag="VN788_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21396.1"
                     /translation="MGSRDSSAKTSLKLKAGEWIEVRSRDEILATLNEQGRLDNLPFM
                     PEMLQYCGQRLRVFKRADKTCDNIKEWSIRRMKDSVHLEGVRCDGSRHGGCEAGCLIF
                     WKEAWLKRAESRLVPPESLRGTAVVPTGSRLCTVESILAASQSRKAEGESVYFCQATE
                     LRNFTSHMRAWNPGQYIRDLRSGNLDSGLAGDFKGQRALDVVLAITRLGRALIITLFN
                     TIQEKRHALPYPHIIGTLDKTPIEALDLQPGELVQVRSKEEIFATLDARHRNRGLLFD
                     SEMLPYCGGIFRVLRRVHQIVDEKTGKMMHMKYPCIVLEGVVCQSDFHRFCPRAIYSY
                     WREGWLRRASAETVSEAEQYPTVTCQQC"
     gene            complement(3130..5085)
                     /locus_tag="VN788_12520"
     CDS             complement(3130..5085)
                     /locus_tag="VN788_12520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21397.1"
                     /translation="MPERHNGQIEYGSNDAGLYSGAGDPPVLLKKRNLFDVAVYVLLI
                     GLGALAFVLVRRSSDFVDDAYYFELAKSISEGTGYGFNFKPQTMVPPGFPLLLALLNV
                     TIGSSYLVLVRLMTVFATVALITAYELLRSEENQAVAAVSCILLGSAPLLFDFSTRNV
                     FSDLPYFFTSMLLLLTVARLDLGAGWHSRRMLLWLLCGASMLSSVLIRSTGIALVGGM
                     FAWLAVSLFRERMTALPRLKLFIPLMAAALILQMMWMFWAAKNQFHEWPIHGYQENYL
                     SQLKLKDGNNPELGLATWKDVLLRPLDNADDNAAAMVTLLTRRWVGALWYSPATSVPI
                     ALALLGLGSSFWKTGGGLVEWYFVCYEAMFLFWPWAFEWRFMLPVAPLACLYIWRGGA
                     LLWRLTRSLPRQVALSLFMLATVSCLSAIEWGWRLQNPKVRWPIAFWTLAAFLSIGLF
                     WGGRDLLERLSLLFAKIVSVRGKLIPLWQVLGYTVVAILFIEGVAMQVKIGRANLHFH
                     LETAAFYPDIEAAEWLKAHSALTAIVMARKEDLVYHYSGRRVVWFPPTTNAGILMDGI
                     ERYHIEYVIVTEGNDSYWKPSVRECFNAYAQVYPNMFRIVHEGPRSWVLARSSDMNHD
                     PLKVHTFQSTPRRHNRWVVAIQARKRA"
     gene            complement(5236..6300)
                     /locus_tag="VN788_12525"
     CDS             complement(5236..6300)
                     /locus_tag="VN788_12525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HXU21398.1"
                     /translation="MNTGSSPLVSILTPVYNGAEYLAECIESVLAQTYPNWDYTIVNN
                     CSTDESLSIAQNYAARDPRIRVVSNDRFLRIIENHNHTIRQISPESKYCKFVFGDDWL
                     HPSCVQEMVRIAEQQPSVGLVGAYAMDGRSVILEGPLYPCPRISGREVCRHTLLGGGY
                     MFGTMTSLLVRSDLIRKRTAFFNEENLHADLEACFDLLRESDFGFVHQVLTFSRPREA
                     SNGAFAKDFDSLPLGYLVSFLKYGPAFLEEAEYRKRWKALHHMYHRMLAKNILRIRPR
                     QFWKYHQDTLAACGSGIDRFLLAISVIAELASQISHPLKAIERAQCWWSRALDRISRA
                     NGHPERRLPRSRDPKTAGDL"
     gene            complement(6366..7664)
                     /locus_tag="VN788_12530"
     CDS             complement(6366..7664)
                     /locus_tag="VN788_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU21399.1"
                     /translation="MSSQISVEKLSKQYRIGLANRHSTLRESLIGLMTLSFLRANAHE
                     ETIWALQNVSFSIKRGEAVGIIGRNGAGKSTLLKILSRITNPTSGIVAVDGKVASLLE
                     VGTGFHQELTGRENIYLNGSILGMAKKEVDRKLDAIVEFSGVAKFLDTPIKRYSSGMR
                     LRLGFAVAAHLDPDILIVDEVLAVGDAEFQKKCLNTMENLRGGARTVLFVSHNLAAVE
                     NLCERCIWIDGGQIRMDGETRSVIEAYMGSVSGAGTVSSELEDVPNRRGSGKVRYRAV
                     EFISPERNAARFIRAGDPVVMRFHYEAAESIPSASFGFRMYTDMGTLVTDTSTSHHGI
                     HVPLEAGKAYLDLEIDCLNLLPAKYTLSLWLTDMDGVEVYDNLEHAVTLEVEASNIYQ
                     SGKVIDSRLGIVFFPQKWNLAGISGRAEQLTASLVSPGRS"
     gene            complement(7699..8484)
                     /locus_tag="VN788_12535"
     CDS             complement(7699..8484)
                     /locus_tag="VN788_12535"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU21400.1"
                     /translation="MRQGSASELWHYRELLYFFAWREIKLRYRQAALGVAWAVIQPLF
                     NMIMFALLFGRLAGMPSDGIPYPIFCFCALVPWTYFSTVLGLASNSLVSNESLLTKVY
                     FPRVLLPAGTALAGLLDFVIGSLLLVCLMFYYHVRLSWALLFSPLVILVMVLLTVGVS
                     MLMAAANVRYRDVRYALPFLIQIWLFATPIIYPATMIPARYRPILALNPCWGVVDAFR
                     ACLFPNLPIDFRLIGASIGVTLVVFVAGAYYFHKAERSFADVI"
     gene            complement(8499..9152)
                     /locus_tag="VN788_12540"
     CDS             complement(8499..9152)
                     /locus_tag="VN788_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012569109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HXU21401.1"
                     /translation="MIHLNLDKGDAGPLRILCVGCHSDDIEIGCGGTILRLTERHSGC
                     VFHWVVLSATGIRQSEAQGAADLFTGTTGLRKTLLKSFPDGYMPFFGAEVKAVFEELK
                     QSVSPDLIFTHNRKDAHQDHRLVGEMTWNTFRDHLILEYEIPKYDGDLGQPGVFVPLQ
                     QEICQKKVRYIMDSFQSQHEKRWFQPDTFLSLMRLRGMECNSPSGYAEAFYCRKLVL"
     gene            complement(9149..9970)
                     /locus_tag="VN788_12545"
     CDS             complement(9149..9970)
                     /locus_tag="VN788_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017744958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXU21402.1"
                     /translation="MKVVLFCGGAGMRLRGYADDIPKPMVQIGTRPILWHLMKYYAHF
                     GHKDFILCLGHKGNVIKDYFLHYDESVSNDFIWSQGGKTIQFLNRDIDDWTITFVETG
                     VTATIGERLKAVEPYLQGEEVFLANYGDGLSDLPLPDMLRKFEQSDAVASFILVQPTA
                     SFDIVDAGPENLVTKISALTRTDIWINGGFFALRNEVLKYVEPGDELVREPFQRLIDK
                     RALLGYKYKGFWQCMDTFKDKQRLDELNQGVAPWKVWNRNSASLADDSRMASAGA"
     gene            complement(9993..11267)
                     /locus_tag="VN788_12550"
     CDS             complement(9993..11267)
                     /locus_tag="VN788_12550"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU21403.1"
                     /translation="MATMTLSPETQQEQAATKSCRFCGAGLRQTFIDLGMSPLCETYP
                     SSADLNRGETYYPLHVYVCEKCFLVQLEQYESAENIFSDYPYFSSYSDSWLKHADNYC
                     GKMTTRLGLNSGSLAVEVASNDGYLLQYFVKRHVPVLGIEPAANVARVAVEKGVPTLV
                     RFFGVQVAEELAAEGRCADLVLGNNVLAQVPDLNDFVEGLRILLKPEGVLTLEFPHLL
                     RLIDHNEFDTIYHEHFSYFSIITTVRILEAHGLKVFDVEELTTHGGSLRVYACRAENQ
                     AQAIEQSVQRLIEEEERAGLASAEGYKTFARQVKQTKLALVEFLLTAAREGKTVAGYG
                     APGKSATLLHYCGIGKDLIEYTVDRSPHKQGRFLPGSHIPIYHPDRIRQTKPDYVVIL
                     PWNLKHEIMEQLQFIREWGGRFVVPIPKVAIY"
     gene            complement(11292..12344)
                     /locus_tag="VN788_12555"
     CDS             complement(11292..12344)
                     /locus_tag="VN788_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009787800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU21404.1"
                     /translation="MKILLTGHKGYIGAVGGLILRSAGHEIVGLDTDLFAGCDFGEAL
                     PEIPEIRKDIRDLTKADLKDFDAVVHLAALSNDPLGNLDASLTFDINYRASVRLAELA
                     RETGVRRFVFSSSCSTYGAAGDAFLDETASLNPVTAYAESKVLVEREVANLASDSFSP
                     TYLRNATAYGASPRLRLDVVLNDLVACAYTTGKVYVKSDGTPWRPIVHIRDIMAAVVS
                     VLEAPVDVIHNETFNVGQTDENYRISELADIVAETVPGCRIEYAPGGGPDKRCYRVSC
                     DKIQRVLSNFHPSWNARKGAQELYAAYRAAGLSSTALDHGRYTRISEIQKLRKAGMLD
                     DSLRWIRQPVEAAAIA"
     gene            complement(12413..13756)
                     /locus_tag="VN788_12560"
     CDS             complement(12413..13756)
                     /locus_tag="VN788_12560"
                     /inference="COORDINATES: protein motif:HMM:NF027579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HXU21405.1"
                     /translation="MGLLKELDQRSPEELGREMYDFATKLFPICRSITGDGIRQTLTS
                     IGTRLALRTVDVPTGTPVFDWIVPKEWNIRDAYIKKPGGKRVVDFKRCNLHVVSYSTP
                     IHATMPFTELKRHLFTLPGNPDYIPYRTSYYREDWGFCVSHNQMLALEDCDYEVHIDS
                     TLEDGRLTYGEYYLPGCSAEEILISCHVCHPSLANDNLSGLAVATALAQFLSGRELRY
                     SCRFLFIPGTIGAITWLAQNRDTAGRVRNGLVLTCIGDAGGFHYKKSRRGNAEIDHAA
                     AHVLHHHHEAGEILEFSPYGYDERQYCSPGFDLPVGCLMRSVWGTFPEYHTSADNLDF
                     IQPLRLAESLRVCTAIFDVLENNRRYCNQKPYCEPQLGRRNLYQSTAGGEIPARLWVL
                     NLSDGKHTLLDIAERSGIPFPAICDAADLLCQNGLLSVLPDGSGLNNRPNVTSGK"
     gene            complement(13763..14290)
                     /gene="rfbC"
                     /locus_tag="VN788_12565"
     CDS             complement(13763..14290)
                     /gene="rfbC"
                     /locus_tag="VN788_12565"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015784348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HXU21406.1"
                     /translation="MIFHQTKLSGVFEIDVERKSDERGFFGRSWCQREFEKMRLNSNV
                     AQCNVSFNKRKGTLRGMHYQATPFAEAKVVRCTRGAIYDVVLDLRPDSATFKDWVAVV
                     LTTANGRMVYVPEGCAHGFLTLEDMTEVFYQMSEFYDADSARGVRWDDPAFRIVWPGD
                     VDVISERDRTYPNFG"
     gene            complement(14828..15622)
                     /locus_tag="VN788_12570"
     CDS             complement(14828..15622)
                     /locus_tag="VN788_12570"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006563630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HXU21407.1"
                     /translation="MKEKSMTPILLREADGPLAILTLNRPDKRNALSLEMMLELIRAL
                     DEIAGERTVRAVILKAAGKVFSSGHDLCELTRRAIEDYRHIFDVCTQLMEKVQSIPQP
                     VIAQVQGIATAAGCQLVATCDLAIASDTASFATPGVKIGLFCTTPMVALSRAIGRKRA
                     MEMLLTGKFVDAQTAVDWGLVNRAVPAGQLESETRELAHQITAASAFTVSLGKQAFYS
                     QIDLDQSKAYAYAKEVMSMNALAAAAQEGIAAFLEKRQVRTEKIVV"
     gene            complement(15664..>16305)
                     /locus_tag="VN788_12575"
     CDS             complement(15664..>16305)
                     /locus_tag="VN788_12575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014267101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HXU21408.1"
                     /translation="PARLDFVRANDVCIQCHSQGRPLKNPIEGKYYDWAVGFHVGMKL
                     QNFWQLEDHKLGETTFTHFADGTAHKNRMQGNDFVQSLMYRRGVKCSSCHDVHGTEND
                     ALLIKPASVMCLECHGPGSPNGPHTATVEEHTHHKSGSPGNECVACHMPKIEQTIADI
                     NVRSHTFRFITPAMTDAYKIPNPCNVCHKDKSTAWATAELKTWSNVSPWRVSP"
BASE COUNT         3810 a         4672 c         4212 g         3611 t
ORIGIN      
        1 ccgcgagaat gcaaacgctc cgccagtttc ttcacctgat tgatgctggc gtaaaaagat
       61 gaacggccac gagccagcgc ggtgaacctg ccaatgtcca cctctaggta gagcatctca
      121 aggtcaccct cacaattgcc ggtggccact cgcataagca ccctgggccg cagcaacaga
      181 cccttagcag tcgctaggag cttccattcc cagctttgct tgattatcga atacgcttca
      241 gatttctccg tgtgcaatac cgtctcatat ggcaacaacg ccgctgattc ttttcccgcc
      301 cctcgatata agcgtcgcgc ataggcccag ttcagccggt ctttcggcat gtagtgctgg
      361 aactgcaatc gcggctcata ccataacttc cacccagcca gccgcaatct gtagcacaat
      421 tcggtgtcct cgcccgccgt gagggagccc ccgctgcggc ctggaaccag aatagggatg
      481 ttcttaaatt ccaactgctg ccatgctgac ttgcgtaatg tcataccggc ggtccaaaga
      541 cttcgagtct cggtacactc gcccccaagc cacgtttccg gagagatcgc gtattgggcc
      601 ttacaggcgg ggaaccaact tggagggctc acttcaaaaa ctggagtgct caaaccgccg
      661 agtgcgccaa cgtcagcatg gcttcgcagg aagctggccg ttaattctac ccactggtcg
      721 gtaacccaat tgtcgtcatc cacgaagctt accaattcat aacgtgcttc cgcgattccc
      781 cgttgccggg cgaacgcagt tccgggccgc tgttcccaaa caatgcgtag cggcgcgaaa
      841 tgatattcgc cccaacattc ccgggccact gtcgcagttc cgtccgtcga cgcattgtcg
      901 ataataagaa cctcccaagg gatgttcttg ctgaccttct gcgccacaag atgcgcaagc
      961 gtagcaggta accgggcggc actattatga caacaaatga cgacactgac tccgaatggg
     1021 aactccgtgg attgcatatc agatcttccg cacggaaggg gcgctcccgt ttagcccgct
     1081 acgtcggcat tccaaatccc cgatactgca tttgcccagc cagcaagacg tatctattct
     1141 gtgcgccagc agtttcaaca tcgaatcaag agggaggata gcatacgcac taggatcgcg
     1201 atattcattt agagagcgcc gggttggaat gcgtacccca caactcgacc gcgcggcctg
     1261 agttaaggaa caagagagtt cgtgcgcagt cttcccgcga gcgcaaaaat catccaaaca
     1321 aataactgtg tgtcatgtca tccgcatcgg cctcatgctg atatcggtga tttcggccgt
     1381 ggagggaagc gtgagtgcct gaacgacgac agacgctatg tcgtccggtt gcaaaagcat
     1441 ttccgggcgg tagttttttc cttcctgccg gtgcagaacc tcctgcatcg gtgttgcggt
     1501 acgtcccaga cacaccgtga gcactcgaat tcctctcccg ttcacctcct cacgcaggct
     1561 gtcggcgacg gctttgagcg catgtttagt ggcactgtat tgccccactt ccggccgttt
     1621 tgccgtcagc gcgaggctgg aattgatgaa tacgatctgg ccacgggcgg cagtcaagag
     1681 gggcagtaaa tgctgtgtca gaacgtacgg tgcacggacg ttgacgttat attgaatatc
     1741 caaatcttca acgcgggcgc gttccattgg gtcgtggcga agaagaccgg cgctgtgaac
     1801 caaaatgtcg agccggccgg cttctccttt caggtgcgtg aggagcggtt gaagattctc
     1861 ttcagcagtc aagtcgattt gaaaacctcg aatgtgcgaa aactgaccgg cagcggcaac
     1921 agtctccgct agcgtgacag gattccttcc gaccacacat acctgcgctc cttgacgcga
     1981 caatcccaga gcaatagccc gtccaattcc gctgcttcct cccgtcacta cggccacatg
     2041 tccagccaga caatcagctt cttgcacagt ccggccctca gcactgttga caggtcacgg
     2101 ttgggtattg ctccgcctcg gaaacagttt ccgcagaggc tcgcctgagc caaccctccc
     2161 gccaatacga gtatattgcc cgagggcaga atcgatggaa gtcagattgg cacaccacgc
     2221 cttcaagcac gatgcaggga tatttcatat gcatcatttt gccggtcttc tcatcaacga
     2281 tctgatgcac tcgccgcaat accctaaaaa tgccaccgca atacgggagc atttccgaat
     2341 caaacaagag gccgcggtta cgatgcctcg cgtcaagggt cgcgaagatc tcttccttgc
     2401 ttcgcacctg aactaattcg cctggctgaa gatcgagtgc ctcaatgggc gtcttatcca
     2461 gcgtgccaat gatgtgtgga taagggagtg cgtgtcgctt ctcttgtatt gtgttgaaca
     2521 acgtgatgat tagagcgcgg cccagccgcg ttatggcaag gaccacatcg agcgcccgtt
     2581 gccctttaaa gtcacccgcc aagccagagt cgagattgcc agaacgcagg tcgcgaatgt
     2641 actggccggg attccaggcc cgcatatgag aagtgaagtt gcgtaattcc gtggcttggc
     2701 aaaaataaac gctctctcct tccgccttcc tcgactggct ggcagccagg atgctctcca
     2761 ccgtgcagag tctggagcct gtaggaacca cggcagttcc tcgtaaactt tctggaggta
     2821 ccaacctact ttcggcccgc ttaagccaag cctctttcca gaaaatcagg cagccggctt
     2881 cgcatccacc atgcctggat ccatcacatc gtacaccttc caggtgtacg gagtccttca
     2941 tccggcgaat gctccactct ttgatattgt cgcaggtctt atccgcgcgc ttaaaaaccc
     3001 gaagcctctg cccacagtat tgcaacatct ccggcatgaa tgggagattg tccaagcggc
     3061 cctgttcgtt caaagttgcc aggatttcat cccgactccg gacctcgatc cattctccgg
     3121 ccttcagctt caggctcgtt ttcgcgcttg aatcgcgact acccatctat tgtgtctcct
     3181 tggcgtggac tgaaaggtgt gaaccttgag tggatcatga ttcatatcgg aggaccgcgc
     3241 caacacccaa ctgcgtggcc cctcgtggac gattcggaac atattaggat aaacttgggc
     3301 atacgcgttg aagcactccc tgactgatgg cttccaataa ctgtcgttgc cctcggtcac
     3361 aatgacatat tcgatatggt atcgttcaat tccatccatc aaaatgccgg cattggtggt
     3421 cggcggaaac caaactactc tgcgtccact gtagtgatag acaaggtcct ctttgcgggc
     3481 cattacaatc gcggtgagcg cactgtgtgc tttaagccat tcggccgcct caatgtccgg
     3541 gtaaaaagcc gccgtttcca gatggaagtg gagatttgcg cggccgatct tcacttgcat
     3601 agccacgcct tcgataaaca gaatcgccac gacagtataa ccgagaacct gccagagagg
     3661 aatcagcttt cctctgactg acactatttt tgcaaacaga agagacagtc tctccagcag
     3721 atcccgccca ccccaaaaca gtccaattga aaggaaggca gccaatgtcc agaatgcaat
     3781 gggccaacgc actttcggat tctgcagacg ccagccccac tcaatcgcac tcaggcaact
     3841 aacagtcgcc agcataaaaa gagacagagc cacctggcga ggtagactgc gagtcagacg
     3901 ccagagcagt gccccacccc tccatatgta gaggcaggcg agtggcgcca ccggcaacat
     3961 gaatctccac tcgaaggccc aaggccaaaa cagaaacata gcttcgtaac agacaaagta
     4021 ccactccacg agtccgccac cggttttcca gaacgagctt cccaagccca agagtgccaa
     4081 cgcgatgggt accgaagtcg ctggcgaata ccagagtgcc ccgacccacc tccgcgtgag
     4141 cagggtcacc atagcggcgg cgttatcatc agcattgtct agaggtctca ataggacatc
     4201 tttccatgtg gcgagcccca attcagggtt gttgccgtcc tttagtttta gctgggaaag
     4261 gtagttctcc tgatacccat gtatcggcca ctcatggaac tggtttttgg ctgcccagaa
     4321 catccacatc atttggagta tcaacgcagc cgccataagc gggataaata gcttcaggcg
     4381 aggcagcgca gtcattcgtt ccctaaaaag cgagaccgcg agccacgcga acattcctcc
     4441 tactaatgcg attccagtgg acctgatgag gacggatgaa agcatcgaag cgccacacag
     4501 caaccacaac aacatccgtc tcgagtgcca gcccgccccc aaatccaggc gcgccacggt
     4561 taagagcagg agcatgctcg tgaaaaaata cggcaggtcg gagaatacgt ttctcgtaga
     4621 gaagtcgaaa agcaagggag cggacccgag taagatgcat gatacggccg caaccgcttg
     4681 attctcttcg gaacggagta gttcgtaggc ggtgatcaga gctacggtag caaagaccgt
     4741 catcaagcgg accagcacaa gataactgga gcctatcgta acgttcaaaa gcgctagtaa
     4801 aagcggaaac ccagggggaa ccatggtttg cggcttgaag ttgaacccgt accctgttcc
     4861 ttcggatata gacttggcga gttcgaaata ataggcgtca tccacgaagt ctgaggatct
     4921 ccgaacgaga acgaacgcca gcgcaccaag accgatgagc aacacgtaga ccgctacgtc
     4981 gaacagattg cgcttcttca agagaaccgg gggatccccg gcgccggagt atagcccggc
     5041 atcgttggaa ccatattcta tctgaccgtt gtgcctttcc ggcattaatc gcctctactg
     5101 tttgcttgga aatgcgatgg taattgtaca aatatccgct cgccgcgaac gaggcgaagc
     5161 agctgctctt ggggcacttc ctacttcaga gaaaacgtag ctgaactagg caggatcgta
     5221 cctttcaaca agttcttaga gatctccagc cgtcttcgga tctcgacttc gcggtaatcg
     5281 ccgttccgga tgcccattgg cccgggaaat cctgtccagc gcccgagacc accaacattg
     5341 ggcgcgctcg atggctttga gcggatgaga aatctgactg gccaattcgg caatcactga
     5401 tatcgctaag agaaatcgat ctatcccgct accacaagcc gccagggtat cctggtgata
     5461 tttccagaat tgccttggac gaatgcgaag aatgttcttt gccagcatgc gatgatacat
     5521 atggtgaagt gccttccacc gcttccgata ctcggcctcc tccaaaaaag ccggaccgta
     5581 tttcaagaaa ctgacaagat aacctagggg aaggctgtca aaatctttgg caaatgcacc
     5641 gttagatgcc tctcgcggcc ggctgaaagt caagacttgg tggacaaaac caaaatccga
     5701 ttctcgcaag agatcaaagc acgcctccag atctgcatgc aggttctcct cgttgaagaa
     5761 agccgttcgt ttccggatca ggtcagaacg caccagcagc gatgtcatgg ttccaaacat
     5821 gtatccgcca cccaggagag tatgccggca cacttcccgg cctgaaatac ggggacatgg
     5881 atataaaggc ccttccaaaa ttacagaccg gccgtccatt gcataggcgc cgaccaatcc
     5941 aaccgaaggt tgctgctcgg caattcggac catctcctga acgcaagaag ggtgcagcca
     6001 gtcatcgccg aaaacgaatt tgcaatactt ggattcggga gatatctggc ggatggtgtg
     6061 gttatggttt tcgatgatcc gcaagaagcg atcgttgctg actacgcgga tgcgcggatc
     6121 tcgggccgca tagttctgcg cgatcgagag cgactcgtcc gtactgcaat tgttgacgat
     6181 ggtgtaatcc cagttcgggt aggtctgcgc cagcacactt tcgatgcatt cagcgaggta
     6241 ctccgctccg ttgtagacag gagtcagtat gctcaccaaa ggcgacgatc cagtgttcat
     6301 ttcttatact ctatctcgca acagaataga gaggcgcgga tcttcggcgc gtacacttca
     6361 tcttgctaag atcgacccgg gctcactaga ctggccgtca actgctccgc tcgtccagag
     6421 attcccgcga gattccactt ctgtggaaag aatacgatgc cgagacggct gtcgatgacc
     6481 tttccggact gataaatgtt gctggcctca acttctaagg tcacagcgtg ctcaaggttg
     6541 tcatatacct caactccgtc catgtccgtg agccacaagg agagcgtgta tttcgcaggc
     6601 agtagattga gacagtcgat ctccaggtcc aagtaagctt tcccagcttc cagggggaca
     6661 tgaatgccgt gatgcgacgt gctggtatcg gtaaccaggg tacccatgtc tgtgtacatt
     6721 cgaaagccaa agcttgcaga cgggatcgac tcggcagcct cgtaatgaaa tcgcattacg
     6781 accgggtcgc cagcccggat gaaccgcgcg gcatttcgtt ccggtgaaat gaattcaaca
     6841 gcgcgatagc gaactttgcc gctacccctc cgatttggga cgtcctctag ctcagaggag
     6901 actgttccgg cgccactcac gctgcccatg taagcttcaa tcacagaccg ggtttcaccg
     6961 tccattcgaa tttggccgcc atcgatccag atacaccgtt cgcacagatt ctctacagcc
     7021 gccagattat gcgagacgaa cagcacagta cgggcgcccc ctcgcaagtt ctccattgtg
     7081 tttaaacact tcttttggaa ttcggcgtcg ccaaccgcca acacctcgtc aacgatcaag
     7141 atgtccggat ccagatgagc ggccaccgca aaacccaaac gcagacgcat tccggatgaa
     7201 tatctcttga tcggtgtatc gagaaacttg gcgacccctg agaactcgac gatcgcatcc
     7261 agtttccggt ccacctcctt tttggccata cccagaatgg agccattcag atatatgttt
     7321 tcccggccgg tgagctcctg gtgaaacccg gtgccgactt ccaacagcga agcgactttc
     7381 ccgtcaaccg ccactatgcc ggacgtgggg ttcgtgatcc tggaaaggat cttaagcaat
     7441 gtgctcttac ccgcgccatt tcgtccgatt atgcctacag cttcgccgcg cttgatcgaa
     7501 aaggacacgt tctgcaatgc ccagatggtt tcctcatgcg cgttcgcgcg aagaaaagat
     7561 aacgtcatca ggccgatcag agattcgcgc aatgtcgagt gccgattagc aagaccgata
     7621 cgatactgct tcgataattt ctcgacacta atttgagaac tcataagcgc atttgccggc
     7681 ctttcgcgtc catcctggtc aaattacgtc cgcgaagctc ctttcggcct tgtgaaagta
     7741 ataagcaccg gccacgaaca ccaccaatgt cacgccgatc gaggcaccga tcagccgaaa
     7801 atcgataggc agattcggga acaggcaggc gcgaaaagca tccactaccc cccagcacgg
     7861 attgagcgca agaatggggc ggtaccgcgc cggaatcata gtcgccggat aaataatagg
     7921 agtggcgaat agccagatct ggattagaaa cggcagcgcg taccggacat cgcgataccg
     7981 tacgttcgct gcagccatca gcatactgac tccaaccgtc aacaggacca tgaccagaat
     8041 aacgagcggg ctgaatagca gtgcccagct taggcgcacg tgataataga acatcaagca
     8101 caccaacagc agcgatccga tgacaaagtc cagcaggccc gccaaggccg tgccggcagg
     8161 caggagaacc cgcgggaaat ataccttcgt aagcaacgac tcattactga ccaagctatt
     8221 gctcgccagt cctagcacag tggaaaaata tgtccagggc accagtgcac agaaacaaaa
     8281 gatgggataa gggataccgt cgcttggcat gcccgccaga cgcccgaaga ggagagcgaa
     8341 catgatcata ttgaacaatg gctggatcac ggcccatgct acgcccaaag ccgcctggcg
     8401 atagcgcaac tttatctccc gccaagcgaa aaaatacagc agctcgcggt aatgccataa
     8461 ctcactggcc gaaccttgtc tcatccagct tgctggtttc acagcactag ctttcggcag
     8521 tagaaagctt ccgcataacc actgggagaa ttacactcca ttccccgcaa tctcatgagg
     8581 gaaagaaatg tgtcgggttg aaaccagcgt ttctcgtgct gggattggaa ggaatccatg
     8641 atgtaacgaa ccttcttctg gcagatctcc tgttggagcg gcacaaacac tccgggctgt
     8701 cccaaatcac cgtcatattt agggatttcg tattccagaa tcaaatggtc gcgaaacgtg
     8761 ttccaagtca tctcccccac aaggcggtga tcctgatgcg catccttacg gttatgcgtg
     8821 aagatgaggt ccggagatac cgactgcttc aactcttcga acacggcttt cacttctgcg
     8881 ccgaagaacg gcatatatcc atccggaaag ctcttcagta gcgtctttcg taaaccagtc
     8941 gtgccagtga atagatcggc cgcgccttgc gcctccgact ggcggatccc ggtggcgcta
     9001 aggaccaccc agtggaatac gcacccggag tgccgctcag tcagcctgag gattgtaccg
     9061 ccgcagccga tctcgatatc gtcagaatgg cagcccacgc aaagaatccg caacggtcca
     9121 gcatcgcctt tgtccaaatt taggtggatc atgctccagc cgacgccatc ctcgaatcgt
     9181 ccgcaagcga ggctgagttc cgattccaga ccttccacgg cgcgacgcct tggttcagct
     9241 catccaggcg ttgtttgtct ttgaaggtgt ccatgcactg ccagaagcct ttatacttgt
     9301 accccaacaa agcgcgctta tcgatcaacc gctgaaacgg ttccctgaca agttcgtcgc
     9361 ctggctcaac atactttaaa acttcgttac gcagcgcaaa gaagcctccg ttgatccaaa
     9421 tatccgtgcg ggtcagtgcg ctaatcttcg taactaggtt ttccggcccg gcgtcgacaa
     9481 tgtcgaaact tgcagtgggt tgaaccagta tgaaggaagc gacagcgtca ctctgctcaa
     9541 atttcctcag catgtctgga agcggaaggt cgctcaggcc atcgccatag ttcgcgagaa
     9601 atacctcttc accttgcagg tagggctcga cagctttgag tcgctcgccg atcgtggccg
     9661 taactcccgt ctccacgaag gtgatggtcc agtcgtcaat atcacgattt agaaactgga
     9721 tcgtcttccc accttgggac cagatgaagt cgttagagac ggattcgtca tagtgtaaaa
     9781 agtagtcctt gatgacgttc cctttatgcc caaggcagag gatgaaatcc ttgtgcccaa
     9841 agtgagcgta gtatttcatt aagtgccaaa gaatcggccg cgtcccgatc tgcaccatcg
     9901 gtttggggat atcgtcggcg taccctctca gccgcattcc cgcgcccccg caaaaaagaa
     9961 cgactttcat gactcaccct cccttttccg cttcaataga tggccacttt tggaatcggc
    10021 acgacgaagc gtccgcccca ttcccggatg aattgcaact gttccatgat ttcgtgcttt
    10081 agattccagg gtaggattac cacataatcc ggcttcgtct ggcggatccg gtccggatga
    10141 taaatcggga tgtggctgcc tggcagaaat cgcccctgtt tgtgcgggct gcgatcgacg
    10201 gtgtactcga tcagatcttt cccaatgcca caataatgaa gcaacgtggc gctcttccca
    10261 ggagctccat atccagcgac ggtcttgccc tcgcgcgcgg ccgtgagcag gaattccact
    10321 aacgccagct tggtttgctt cacctgccgg gcaaaagtct tgtagccttc cgccgacgcc
    10381 agcccggcgc gttcttcctc ttcgattaac ctctggacgc tctgctcaat ggcctgtgcc
    10441 tggttctctg cccggcaggc gtacacgcgg agcgagcctc cgtgcgtcgt caattcttcc
    10501 acatcgaata ccttcaggcc gtgggcttcc aggatgcgca ccgtggtgat gatcgaaaaa
    10561 tacgagaagt gctcgtgata aatcgtgtca aactcgttgt ggtcaatcag ccgcagcagg
    10621 tgcgggaact ctagcgtcag gactccctcg ggcttaagga ggatccggag cccctccacg
    10681 aaatcgttta ggtcggggac ttgcgcgaga acattattgc ccagaaccag atcggcgcaa
    10741 cggccctcgg ccgccaactc ttccgctact tggacgccga agaaacgcac cagcgtggga
    10801 acgccttttt cgaccgcaac cctggccaca ttcgccgccg gctcaatacc gaggaccggc
    10861 acgtgccgct tgacgaaata ctgcaacagg tagccatcgt tactggctac ttcgacggcg
    10921 aggctgccgc tgttcaggcc caatcgcgtc gtcattttgc cgcagtaatt gtcggcgtgt
    10981 ttgagccatg agtccgaata cgacgagaaa tatgggtagt cgctgaagat gttttcagca
    11041 ctctcgtatt gctccagttg caccaggaag cacttttcgc agacgtaaac gtggagtgga
    11101 tagtaggttt ctccacggtt gaggtcggcg gacgatggat acgtctcgca aagtggggac
    11161 atccccaggt cgatgaaggt ttgccgtaga ccagctccac agaagcggca cgacttggtg
    11221 gcagcttgtt cctgctgcgt ttcgggagaa agcgtcattg tggccatata ctcgttcctt
    11281 tctctaacgg ctcaagctat cgccgccgct tcgacgggct gccggatcca gcgcaatgaa
    11341 tcgtccagca tcccagcttt cctgagcttc tgtatttcgc tgatccgcgt ataacggcca
    11401 tgatccaggg cggttgaact taggcctgcg gcccgatatg ccgcatatag ctcctgcgct
    11461 cctttcctgg cattccagct tggatggaaa ttggacaaaa cccgctggat cttatcacag
    11521 ctcacgcggt agcaacgctt gtccggtccg ccgccaggcg catactcgat tcggcaccca
    11581 gggacggttt ccgcaacgat atcggctagc tcgctgatgc ggtagttctc gtcggtttga
    11641 cctacgttaa aggtctcgtt atggatgacg tccacaggag cctccagcac gcttacaacg
    11701 gctgccatga tgtctcgaat atgcacaata ggacgccaag gcgtgccgtc gctcttgaca
    11761 tacaccttgc cagtggtata cgcgcatgcc accaggtcgt tcaaaaccac gtccaggcgc
    11821 aggcgcgggg acgcgccgta ggcggtggcg tttctcaggt aggtaggact gaaactgtcg
    11881 ctcgccaaat tcgctacttc gcgctccacg agcactttgg actcggcata cgctgtcacc
    11941 gggtttagcg aggcagtctc gtccaggaaa gcgtcccctg ccgctccgta cgtgctgcag
    12001 gaggaggaga aaacaaagcg gcgcacgccg gtttctctcg ccaattcggc cagccggacc
    12061 gatgcccggt aattgatgtc gaaagtcaag ctggcatcca gattgcctag tggatcgttc
    12121 gacaaggcag ctaagtggac cacggcgtcg aaatccttga gatcggcctt cgttaagtcg
    12181 cgtatgtctt tgcggatctc cgggatctcg ggcaacgcct ccccgaaatc gcaccctgca
    12241 aacaaatcgg tgtctaaacc gacaatttca tggccggcgg agcgcaaaat caatcctccg
    12301 acggcgccga tgtaaccttt atggccggtt aatagtatct tcatagctat aattccattt
    12361 cttgagttac cttacgttcc aagcaatctc gaaatttttg aggtcgcgcg aattactttc
    12421 ccgaagtgac attcggtcga ttgttcaagc ctgagccgtc gggcaggacg gaaagcagac
    12481 cgttctggca caacaggtct gctgcatcgc aaatcgccgg gaacgggatt ccggagcgct
    12541 ctgcgatatc cagaagagtg tgctttccgt cagagagatt caatacccag aggcgcgcgg
    12601 ggatctcgcc accggccgtt gactgataga gatttcgccg gccgagttgc ggttcgcaat
    12661 aaggcttctg gttgcaatat cggcgattgt tttcgagtac gtcgaagatc gcggtgcaca
    12721 ctcgtagcga ttctgccagg cgcaagggct gaataaaatc gagattgtct gctgaggtgt
    12781 gatactccgg aaaagtgccc cacacgctcc gcatcaggca gccaactggc agatcgaaac
    12841 ctggggagca gtactgccgc tcgtcataac catatggaga aaattccaga atctcgccgg
    12901 cttcgtggtg gtgatggaga acatgcgcgg cagcatgatc gatctcagca ttcccccgcc
    12961 ggctcttctt gtaatggaat ccaccagcgt cgccaatgca cgtaagcacc aacccattcc
    13021 ggacacgacc ggcggtgtca cgattctgag ccagccaggt aatagcgcca atcgtcccgg
    13081 ggataaataa gaaccggcac gaataacgaa gctcccgccc ggacagaaac tgcgccaacg
    13141 cggtcgccac agctaatccg gaaagattgt cattggccag agatggatga cagacatgac
    13201 aagagattag aatctcctcg gcggaacacc ctggcaaata atactcgcca tacgtcaaac
    13261 gtccatcttc gagagtagag tcaatgtgta cctcgtaatc acagtcctct agcgccaaca
    13321 tctgattatg ggacacgcag aatccccagt cttcccgata gtaagacgtg cggtaaggaa
    13381 tatagtcggg attcccggga agggtaaata agtgccgttt aagttcagtg aacggcattg
    13441 tcgcatgtat cggagtacta taactcacca catgcagatt acagcgctta aaatcgacga
    13501 cccgtttacc acccggcttc ttgatatatg catcgcgaat attccattct tttggcacta
    13561 tccagtcgaa aacgggggtt cctgtgggta cgtctacagt ccgcaaagca agccgggtcc
    13621 cgattgatgt tagggtctgc cggattccgt cgccggtaat gctccggcag atcgggaaaa
    13681 gtttcgttgc gaaatcatac atttcccggc ctagttcttc cgggcttctt tggtcaagct
    13741 ccttcagtag gcccatcgct tgttacccaa agttcggata tgtccggtcc cgttcggaaa
    13801 taacgtcaac gtctcccggc caaacaatcc gaaacgccgg atcgtcccac cgaactccgc
    13861 gagccgagtc cgcatcgtag aattccgaca tttgatagaa aacctcggtc atgtcttcca
    13921 aggtcaaaaa tccgtgcgcg cacccctctg gcacgtagac cattctcccg ttcgcggttg
    13981 tcagaaccac tgcgacccag tctttgaacg tcgccgagtc gggtcgtaag tcaagcacca
    14041 catcgtagat agctccccga gtgcagcgga ctactttggc ttccgcaaaa ggtgtcgcct
    14101 ggtaatgcat gccacgcagc gttccctttc gcttattaaa tgagacgtta cactgcgcta
    14161 cattggagtt cagtctcatc ttttcgaact ctctttggca ccaactgcgg ccaaaaaatc
    14221 ctcgttcgtc ggatttgcgc tcaacgtcga tctcaaatac cccagataat ttggtttgat
    14281 gaaatatcaa tctctcaacc tgttcccaga aaggttctac ttagggtgag actcttttca
    14341 aatggcacta atccaggatg aaccagagcc aaccggcaaa acgcaccggg agggcgataa
    14401 aaattcgtcg gttagaactc cgctgatctg ctccgcgagc aagtggagca ctgttttcga
    14461 ccccgacggc aggggaaggg caagctaccg cccatttact cccactttcc ggtttccccc
    14521 taatctatta gagacgttcc gaatttgcgt gaggatcggg ccgccttcaa acccgaacta
    14581 tgtatttttt taaagtatac aatagacaat aagagcgtcc gcatcgaaca cgattatacg
    14641 gtactggtac cttcaaccct atcgaggctt tgcagggcca agcagccaaa tcctgccatg
    14701 ccgcttgttt tcttatctct agcttgtgaa cggtatctgg caattccgat ctctgccccc
    14761 ggcccttcgt tgatgtcgat cggaactagc cgtacgattc tcagaaacgg ctaaagataa
    14821 agaaaagtca gaccacaatt ttttcagtcc taacctggcg tttttcgagg aacgccgcga
    14881 tcccttcctg cgccgcggcg gccagagcgt tcatgctcat cacctctttc gcgtaagcgt
    14941 aggctttgga ttgatcgagg tcgatctgcg aatagaacgc ctgctttccg agactcacgg
    15001 tgaaggcgct cgccgcggtg atctgatgag ctagttcgcg ggtctcggat tcaagttgtc
    15061 cggcgggtac cgcccgattc acaagtcccc aatccacggc cgtttgcgcg tccacgaact
    15121 taccggtgag cagcatttcc atggcccgtt tgcggccgat cgcgcggcta agcgctacca
    15181 tcggcgtcgt gcagaacaag ccgattttga cacccggcgt cgcgaacgat gcggtgtcgc
    15241 tggcgatggc tagatcgcag gtcgcgacaa gctggcagcc cgcggcggtc gcgatccctt
    15301 gtacctgggc gatgaccggc tgcgggatcg actggacttt ctccatcaat tgcgtgcaga
    15361 cgtcgaagat gtgacggtag tcctcaatag cgcggcgggt gagttcgcag agatcgtggc
    15421 cggaggagaa gaccttgccg gccgccttca gaatgacggc gcgaacggta cgttccccgg
    15481 cgatctcgtc gagagcgcga atcagctcca acatcatttc aagcgagagc gcgttgcgct
    15541 tatcgggccg gtttaatgtg agaatcgcga gcggtccgtc ggcctctcga agcagaatgg
    15601 gcgtcatgga tttttccttc attccatctt cgcccagaaa cgctcgcgca gatcggctcg
    15661 ccgttaggga cttactcgcc acggcgagac attcgaccac gttttcaact ccgccgtggc
    15721 ccaggcggtc gacttgtctt tgtggcaaac gttgcacggg ttcgggattt tgtaggcatc
    15781 ggtcatcgcc ggggtgatga agcgaaatgt gtggcttcgc acgttgatgt ccgcaatcgt
    15841 ctgttcgatc tttggcatgt ggcaggccac gcattcgtta ccgggactgc ccgatttgtg
    15901 atgggtgtgc tcttcgacgg tagccgtatg cgggccattg ggcgatcctg gcccgtggca
    15961 ttcgaggcac attacgctgg ccggcttaat cagcagcgcg tcgttctccg ttccatgcac
    16021 gtcatggcaa ctggaacatt tgacgccgcg cctgtacata agactctgga cgaaatcgtt
    16081 accctgcatc cggttcttgt gagccgtgcc atcggcgaaa tgagtaaagg tggtctcgcc
    16141 gagcttgtgg tcttccagtt gccaaaaatt ctgaagcttc atgccaacgt ggaatccgac
    16201 agcccagtcg taatacttgc cttcaatcgg gtttttgagc gggcggcctt gcgagtggca
    16261 ttgaatgcag acgtcgttcg cacggacaaa atccaagcgg gccgg
//
LOCUS       DATNDT010000387         3792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1698625, whole genome shotgun sequence.
ACCESSION   DATNDT010000387 DATNDT010000000
VERSION     DATNDT010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1698625"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /locus_tag="VN788_12580"
     CDS             <1..1278
                     /locus_tag="VN788_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21409.1"
                     /translation="LPQGWTATLDAAKDRSTSYHFNVTVPAGATAPSSPADAVLPFPP
                     PLVSFAVNVAVEGYHFTIEKPVESSEAKTTEIDTYPLELVPAVTLTVEPSQVMIPQKR
                     AAAPFALLARVRYHGSTKATVQVGIDAPQGWSVAPISPLDFASAGDQLIRYVVTPPAK
                     IAPNAYPLHPYARIGEETFRTSLEPLPTMPTRDWSQPDDATVHVLNLTVPAELRIGYI
                     AVDNDVIPETLRQVGIQVDMLDEVALAFGDLSRYDAIVVGIRSYELRPDVERSNPRLL
                     DYVKNGGTLLVLYERDFAWNRILPAPYPAKMSAQAARVTDPDSPVRFLAPENPLLNTP
                     NKITLDDFKGWEQERGLYFWGTWDSHYQPILGLTDQGEKEATGGLVYARYGKGVYIYT
                     GLSFFRELPDGVPGAYRLFVNLLSQTRHSTSRR"
     gene            complement(1338..2702)
                     /locus_tag="VN788_12585"
     CDS             complement(1338..2702)
                     /locus_tag="VN788_12585"
                     /inference="COORDINATES: protein motif:HMM:NF017135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050080 - malonyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-CoA decarboxylase family protein"
                     /protein_id="HXU21410.1"
                     /translation="MPGFLSNLLQILRERRGSDMLFLCRHLLSERGEASQTVLAERII
                     KAYMEMSSADRLKFFEMLCRDFGPDPAAISRAAAEYERNPSAETLAPLFSAVEPPRQE
                     LVRRINTATHGTETLVTMRGHLLELAGSKGANFAALDADLRHLFKSWFNRGFLRLERI
                     SWQTPAHVLEKLIHYEAVHEINGWPDLRRRLQADRRCFAFFHPALDQEPIIFIEIALS
                     NQLVGELEPLLDIHAPVIAAEKANTAIFYSINNCLAGLRAIPFGNFLIKQVVVELAAE
                     LPDVKSYATLSPLPRFARALKDDRNADGFTRERLSRLLADYAEDLTAATRERDPVEAF
                     HDLLQTPLQNREILSAPLERLALAYLTEARDGGRLYDPVASFHLANGARLERINAFGN
                     LRPYGLEASFGVTVNYRYLPDEVEENHEQFVRNGQIRLGGGLARDFREVSAAWQSPHR
                     GKRS"
     gene            2932..3561
                     /locus_tag="VN788_12590"
     CDS             2932..3561
                     /locus_tag="VN788_12590"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU21411.1"
                     /translation="MFKRSGRFGGRRWVAWCAAAALIFTLSFDAQAGQKKKKNKDADK
                     AASAQPAAPGLPMPESDKINNDIGEMLAAFQLGKVDMMHKYYSDNVTFVSGEYAPPVV
                     GWQNYVPIYEHQWEAFTGIQLNRRNTLVFIHGDVAWASYQWEFDALLKGQPYSMRGQT
                     TLIFNKVNDNWLIVHNHTSQVYAPPAYAQTPTPTPAPQPSTVPTAAPRP"
BASE COUNT          819 a         1090 c         1137 g          746 t
ORIGIN      
        1 ctgccgcaag gatggactgc gacgctggac gcggcgaaag accgaagcac gagctaccac
       61 ttcaatgtaa ccgtacccgc cggcgcgaca gcgcccagct ctcctgctga tgcggtgctg
      121 ccattcccgc cgccccttgt cagcttcgcg gtgaacgttg ccgtggaggg ctaccatttc
      181 acgatcgaaa agccggtgga atcgagcgaa gccaagacga cggaaatcga tacgtatcca
      241 ctggagctgg ttccggccgt gacgctgacc gtcgagccat cgcaagtgat gattccgcag
      301 aagcgcgcag cggcgccctt tgcgctgctg gcgcgggtgc gctatcacgg cagcacgaaa
      361 gcgacggtgc aagtgggaat cgacgcgccg cagggatgga gcgtcgcgcc gatttcgccg
      421 ctcgattttg ccagcgccgg agatcagctt atccgctacg tggtgacgcc acccgcgaag
      481 atcgcgccca acgcttatcc gctgcatccg tacgcgcgaa tcggtgagga aacttttcgt
      541 acttcgctcg agcctcttcc cacgatgccc acgcgcgatt ggagccagcc ggacgacgct
      601 acggttcacg tcctcaatct cacagtgccc gccgaattgc ggatcgggta catcgcggtg
      661 gacaacgacg tgataccaga aacgttgcgc caggtgggaa tccaggtgga catgctcgat
      721 gaagtggcgc ttgcattcgg cgatctgagc cgatatgacg ccatcgtggt gggcatccgt
      781 tcatacgagt tgcgtcctga cgtagagcga tcgaatccgc ggctgctcga ctatgtgaag
      841 aatggcggca cgctgctggt gctctacgag cgcgatttcg cgtggaaccg aattttgccc
      901 gcgccgtatc cggcgaagat gtcagcccag gcagcgcgcg tgaccgatcc ggattcgccc
      961 gtgcgatttt tggcccccga aaacccgcta ctgaatacgc ccaataaaat cacgctcgac
     1021 gatttcaagg gctgggagca ggagcgtggc ttgtatttct ggggaacatg ggattcgcat
     1081 tatcaaccga tactgggact gactgaccag ggcgaaaagg aagcgaccgg aggtctggtg
     1141 tacgcgcgct acgggaaggg cgtgtacatt tacacgggat tgtcattttt ccgcgaattg
     1201 ccggacggtg tgcccggagc gtaccggctt ttcgtcaact tgctgagcca gacgcggcac
     1261 tcgacatcgc ggcgctagct cgagggctct cgaacacaaa tcgcccggcg cgcggaccat
     1321 ggcgcgccgt gccagcttca gctgcgcttg ccccggtgcg gactttgcca cgccgcagag
     1381 acctctctga aatcgcgagc taagccgccg ccgagacgaa tttgcccatt gcgaacgaat
     1441 tgctcgtgat tttcctcgac ttcgtcaggc aaataccggt agttgacggt caccccgaat
     1501 gaagcctcca aaccataggg tcgcaagttg ccaaatgcat tgatccgttc taggcgcgcg
     1561 ccattcgcca ggtggaatga ggcaacgggg tcatacagtc ttccaccatc ccgggcttca
     1621 gtgagatagg caagagccaa tcgctcgagc ggcgctgaca gaatctcacg attctggaga
     1681 ggcgtttgca acaagtcgtg gaaggcttcg acaggatcgc gttcgcgggt cgcggcagtg
     1741 agatcctcgg cataatcggc caagaggcgc gacaaacgct cgcgcgtgaa accgtccgca
     1801 ttccgatcgt ccttcagagc gcgcgcgaag cgcggcagcg gcgagagcgt ggcgtagctc
     1861 ttaacgtcgg gtaactcggc ggcaagttcc acgacaacct gtttgatcag gaaattgccg
     1921 aagggaatgg ctcgcaagcc cgccaggcag ttgttaatgg aataaaagat cgccgtgttc
     1981 gccttctcgg ctgcgatcac cggcgcgtgt atgtcgagga gcggttccag ctctccgacg
     2041 agttggttcg atagcgcgat ttcgataaag atgatcggtt cctgatcgag cgccggatga
     2101 aagaacgcaa agcagcggcg atccgcttgg agacggcgtc gcaagtctgg ccatccatta
     2161 atttcgtgga ccgcttcata gtgaataagt ttttccagca catgcgctgg ggtttgccaa
     2221 ctaatgcgct cgaggcgcag gaacccgcgg ttaaaccacg atttgaaaag gtgcctcaaa
     2281 tcggcatcga gtgcggcgaa gttggcgcct ttggagccgg cgagttcaag caagtggccc
     2341 cgcatggtga ccagcgtttc ggtgccgtgt gtagccgtgt tgatgcggcg aacaagctcc
     2401 tggcgcgggg gttcaacggc cgagaagaga ggtgccaggg tctcggcgct cgggtttctc
     2461 tcgtattcgg cagccgcgcg gctgatcgcg gcgggatccg ggccgaagtc ccggcagagc
     2521 atctcgaaaa acttgaggcg gtccgccgag ctcatttcca tgtaggcttt gataattctt
     2581 tcggcgagga cggtctgcga cgcttcgcct cgttccgaaa gaagatggcg gcacagaaac
     2641 agcatatcgg agccgcgccg ctctcgcagt atctgcaaca ggttgctgag gaatccgggc
     2701 atgagcatct cattatagcg aggtgccttg ttggcgtcac atgactgtgc ggttagaatt
     2761 tcgcataacc cgctataagt aactgccctc ccgacaccct tctaaaggcc acaaaatcgg
     2821 gcaagcggcc tgtgcgctga tgcgacacat cccctggctt ccactgggag attggttttc
     2881 acttcgtgct atgctgcgac cgattcgcgg gcgaaacaga ggagaagcaa aatgttcaag
     2941 aggtcgggga gatttggtgg gcgccggtgg gtcgcttggt gcgccgcggc ggccttgatt
     3001 tttacgcttt cattcgacgc gcaagcggga caaaaaaaga aaaagaataa ggacgcggac
     3061 aaagctgcgt ccgcgcagcc agcagctcca ggcctgccca tgccggaatc cgacaaaatc
     3121 aacaacgata ttggggaaat gcttgcggcc ttccaactgg ggaaggtcga tatgatgcac
     3181 aaatattatt cggataatgt cacgttcgtg agcggagaat acgcgccgcc cgtggtcggc
     3241 tggcaaaatt acgtgccgat atacgaacat cagtgggaag cttttacggg aatccaattg
     3301 aaccgaagaa acactctagt tttcatccat ggagatgtgg cctgggcgtc gtatcagtgg
     3361 gagtttgacg cgctgctgaa gggccagccg tattccatgc gcggccagac gactttgata
     3421 ttcaacaaag tgaacgacaa ctggctgatc gtgcacaacc acacatcgca ggtttacgct
     3481 ccgcccgcct acgcgcaaac gccgacaccg actccagcgc cgcaacccag cacggtccct
     3541 accgcggcac cgcgtcccta aggcttcggc ttcggggcga ccagccccct agagaccgcg
     3601 cgcgcactcc tgaggcgcaa accctgcggt ttcggcgacg tgcttcaaaa cagcgaagaa
     3661 tggctatgaa acgacgcttt gtccacaccc accgtggacg acttttcttt acaccaacaa
     3721 gtacttgcgc tcgcggaggc ccttgcgtaa gaatattccc tgtatcctcg aagtacgccc
     3781 actcgaccca tt
//
LOCUS       DATNDT010000388         6977 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2405065, whole genome shotgun sequence.
ACCESSION   DATNDT010000388 DATNDT010000000
VERSION     DATNDT010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6977
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2405065"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1314
                     /locus_tag="VN788_12595"
     CDS             <1..1314
                     /locus_tag="VN788_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014098747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HXU21412.1"
                     /translation="FIDLKVVEGRGKALIAMNRSGLIAVVDEKNREKERHHVVYGARL
                     RVEDGQPVTLGQIMCEWDPYTFSILTETGGTIEFKDLQPGVTIEEQVDEVTGLSQLVV
                     TLPPGDEKHQPAILVRDSAGRAKKKYLMPSRAHLMVADGDEVQPGDVLAKIPRETTKT
                     KDITGGLPRVVELFETRKPRDPAVISEIDGIVKYGEIAKGQRKIYVESEDGRTVKEYS
                     IPRGVHINVQEGEHVRAGEPLIDGPLNPHDLLAVLGEKYTQAYLVNSVQEVYRLQGVN
                     INDKHIETIVRQMMRWVKVEDVGDTTFLLEEQIDKFRFREENDRVIRDGGRPATGRPL
                     LLGITKASLSTDSFISAASFQETTRVLTEAAISGKVDYLRGLKENVIMGRLIPAGTGL
                     ERYRNIQLLTEIPKEEPEAMEPELTPEEAAALDFLRKDADAVAES"
     gene            1511..3874
                     /locus_tag="VN788_12600"
     CDS             1511..3874
                     /locus_tag="VN788_12600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU21413.1"
                     /translation="MSKDTLQNQAEQAALSPDAKRQFEQLARAISRSQQNYRDLIDSL
                     DQALFTLSLQGEIRVANLRMSHLLGAPFQEIIGRPLSDFLLSPTPAELLQSLDVLLKK
                     GTWTGVVPVTLKKDGELRHFSCWFQTLVERGEVVALTGWARDVTEQFEIEKRLHQEQE
                     FNRRLIANFPDLIAVLDLQGRFTYISDQVRSILGRSPQQYLGGEFGVRAGPEDKIKID
                     EMFQRVIKGDVSRAQIEFRAPHADGTWRVLLLTAGPLFDENGKISGVVTSTRDVTEQH
                     EIERKLHQEQEFVRRLIECFPDLIVVLDSEGRFKFVSERLKDILGVSPDQYVGKKVGL
                     RMSSDDRAKLSEMLQSTISGRKAQEQIEIRAQHVDGTWKTLRITARPLFDEQGNITGM
                     VSSGRDVTESKQFEQQLAQSEKFAAMGQMMAGAAHELNNPLTAILGISDLLRERATDE
                     TTRRQVDTILQQARRAAGIVLNLLAFSRPSAHGHVKIDVKAAIQETLQIEGTLLAQNN
                     ISVKFEAPQELPLVEGNRRLLTQVFSNLIANAEQSISAARSGGRLDISACCVDGRIRI
                     SFADDGAGIDPEIIGKIFDPFFTTKRPGGGSGLGLTIALAVIKEHGGTIEVQSTLGLG
                     ATFHVFLPPCAQASSDSASSSSASLAPPRSAADVLQGRNVVIVDDEESIREIVAEDLS
                     RRGMKVHTVESSEAALNYLSTNKCEIVICDFNLPGISGEALFEKLLAKENGPRPRFVF
                     MTGELVDSGLVSKFREKGARILQKPFSISVLAALLADFMQGETLPSS"
     gene            complement(3886..4833)
                     /locus_tag="VN788_12605"
     CDS             complement(3886..4833)
                     /locus_tag="VN788_12605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21414.1"
                     /translation="MGGRSSRWLLHISRCALLAIIVGAVPLLSAQDAGNSGQESTVHN
                     SQIPAPKAPTTPRGQKLMLKDGSFQLVREYAVQGDRVRYYSLDRSQWEEIPADLVDWD
                     ATGKAAAQEQRQEAAIVAKVHNQEAARLAQPLDIDASLEVAPRIFLPPGAGLFAFDGK
                     AVLPVPQAETTSKLSRGHFLEQVMIPVPIIPTRHNVSIQGMHAKLRLRTEQPEFYMRT
                     ADARTPEVELMRAKAHGENRQIEKVDELFGQQQTTGATISMQRWEIARGVYRFTLSKP
                     LPPGEYALAEMVGDEGTSLYVWDFGVDAGSQREANHSAK"
     gene            complement(5017..5235)
                     /locus_tag="VN788_12610"
     CDS             complement(5017..5235)
                     /locus_tag="VN788_12610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21415.1"
                     /translation="MVRIKSSQKIFTEDEVANLTGICIEHLRGLARNKHLGTLANAAE
                     TAGTHAGRWLFTNSELMIVTVLQPRCQH"
     gene            complement(5508..6953)
                     /gene="gatA"
                     /locus_tag="VN788_12615"
     CDS             complement(5508..6953)
                     /gene="gatA"
                     /locus_tag="VN788_12615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HXU21416.1"
                     /translation="MSAAAPWTVASVRAALLAKKISTRELAAEFYSRIERRNPKLNAY
                     LTLSPERAYAQADRIDASVARGEDLPPLAGVPMAIKDVISTRGVRTTCASKILENYVP
                     AYDATAVERLEKAGALLLGKTNCDEFAMGGSNENSAYGPVRNPVAPDRVPGGSSGGSA
                     AAVAAGLAVAALGTDTGGSIRQPGAFCGIPGLMPTYGRVSRYGLVAFASSLDKIGPLA
                     GNIADATSVISAIAGHDENDSTSAPVDVSDYASETRKPIQGLRIGVPEDYFAEGIDPE
                     VKEKVQSGIALLERLGCKRVPLRMPHTDFATAVYYVLATAEASSNLARYDGVRYGLRV
                     PGATLTEMYRNTRQGGFGAEVKRRIMLGTYVLSSGYYDAYYLRAQSVRTLVARDFADA
                     FNKADVIVTPTAPTAAFGLGEKTADPLQMYLADIYTVTGSLAGVPGISVPCGKNKTGL
                     PIGMQIFGPHFAEGRVLQVARAFEEAGGFGS"
BASE COUNT         1507 a         2023 c         1954 g         1493 t
ORIGIN      
        1 tttatcgacc tgaaggtggt cgagggccgc ggcaaggctc tcatcgccat gaaccggtcc
       61 ggtttgatcg ccgtcgtcga cgaaaagaac cgtgaaaagg aacgccacca cgtggtctat
      121 ggtgcgcgcc ttcgagtcga agatggccag cctgtcactc tcggtcagat catgtgcgaa
      181 tgggatccct acaccttctc cattctcacg gaaacaggcg gcacgatcga gttcaaggat
      241 ctgcagccag gcgtcaccat tgaagaacaa gtggacgagg tcacgggcct ttcgcagctc
      301 gtcgtcacgc ttcccccagg tgacgaaaaa caccagcctg cgattttggt gcgcgattcc
      361 gctggccgcg ccaagaagaa gtacctcatg ccgtctcgtg cccacttgat ggtcgctgat
      421 ggcgacgagg tgcagcccgg cgacgtgctc gccaaaattc ctcgcgaaac caccaagact
      481 aaagacatca cgggcggtct cccgcgcgtt gtcgagttgt tcgaaacgcg aaagcctcgc
      541 gatccggcgg ttatcagcga aatcgacggc atcgtgaaat atggtgaaat cgccaagggc
      601 cagcgcaaga tttacgtcga aagcgaagat gggcgcaccg tgaaggaata ttcgattccg
      661 cgcggtgtgc acatcaatgt ccaggaaggc gagcatgttc gggctggcga gcctctgatt
      721 gacgggccac tcaatccgca cgaccttctg gccgtactcg gcgaaaagta cacgcaagcc
      781 tacttggtga attccgttca agaggtttat cggctccagg gtgtcaacat caacgacaag
      841 cacattgaaa ccatcgtccg gcaaatgatg cgctgggtga aggtcgaaga tgtgggcgat
      901 accacgttcc ttctggaaga gcagattgac aagttccgct tccgcgaaga gaacgaccgt
      961 gtgatccgcg acggcggcag gccggccacg ggtcgcccct tgctcttggg tatcaccaag
     1021 gcttcgcttt ccacggattc ctttatctcc gcggccagct tccaggagac cacccgcgtc
     1081 ctcaccgagg cggcgatctc cgggaaggtg gattatctcc gcggcttgaa ggaaaacgtc
     1141 atcatgggtc ggctgattcc tgctggcacc ggcctcgagc gctaccgcaa tattcagctc
     1201 cttaccgaga ttccgaaaga ggagccggaa gcgatggagc ctgaactcac gcccgaagag
     1261 gcagcggcgc tcgatttcct ccgcaaggat gccgacgccg tggcggaaag ctagaaacag
     1321 gctactggac ccgggaaagg cttgcctggt cgttttcacg ctgggcaagc cgccgccttt
     1381 cttcaacccc tccctgttga gcatttggca tagcctgcct ttttctttcc tgatttcgcg
     1441 ccgccactct gtaagattcg taaggagcct attcgcaccc ttcgaagtga cgctcggata
     1501 gctcagaacg atgtccaaag acacgctgca aaaccaggcg gagcaggccg ccttatcgcc
     1561 tgacgcgaaa cggcaatttg aacaactggc tcgggcgatt tcccgctccc agcagaatta
     1621 ccgcgacctc atagacagcc tcgaccaggc gcttttcacg ctttcgcttc aaggcgaaat
     1681 ccgcgtcgcg aatctgcgaa tgtcgcacct cctgggggcg cctttccagg aaatcatcgg
     1741 ccgccctctg tccgatttcc ttctctctcc cactccggct gaactgttgc aatcgctgga
     1801 cgtgctgctg aaaaaaggca catggacagg cgtcgtgccg gtaaccctca agaaagatgg
     1861 ggaactgcgc catttttcgt gctggtttca gacgctcgtc gagcgcgggg aagtcgtggc
     1921 cctcaccggt tgggcgcgcg acgtcaccga gcagttcgag atcgaaaagc gtctgcatca
     1981 ggagcaggaa ttcaaccgac gcctgatcgc aaacttccct gatttaatcg ccgttttgga
     2041 cctccaggga aggttcacct acatcagcga tcaggttcga agcattctgg gccgctctcc
     2101 ccaacagtat ttgggagggg aattcggcgt acgcgccggc ccggaggaca aaatcaagat
     2161 cgatgaaatg ttccagcgcg taattaaggg cgatgtctct cgcgcgcaga tcgaatttcg
     2221 cgcgccgcac gcggatggca cttggcgagt tctcttactt actgctggtc cactctttga
     2281 tgagaacggg aaaatcagcg gagtggtaac gtcaacccgc gatgtaacag aacaacatga
     2341 aatcgaaaga aaactccatc aggagcagga gttcgtccgg cgtttgatcg aatgctttcc
     2401 cgatctgatc gtcgttctcg attccgaggg tcggttcaag ttcgtcagcg aacgcctaaa
     2461 agatatcctc ggtgtgtctc ccgaccaata tgtcggcaag aaggttggct tgcgaatgag
     2521 ttcggacgat cgagcgaagc tctcggagat gctccaaagt acgatctctg ggcggaaagc
     2581 tcaggagcag atcgaaatcc gcgcccaaca cgtcgatggc acttggaaaa cgctgcgaat
     2641 cacggcaagg cctctcttcg acgaacaggg caatattacc ggtatggttt cttcggggcg
     2701 ggatgtaacc gaatccaaac aatttgagca acaacttgcc caaagcgaaa agttcgccgc
     2761 tatgggccaa atgatggccg gtgcagcgca tgagctcaat aatccgctta ccgccattct
     2821 aggaatcagc gatctgctcc gcgagcgcgc cacggacgaa accacgcgac gtcaggtaga
     2881 cacgatcttg cagcaggcac ggcgcgccgc cggtatcgtt cttaatctgc tcgctttttc
     2941 acgaccttcc gcgcatgggc atgtgaagat cgacgttaag gcggcgatcc aggagacatt
     3001 gcaaatcgaa ggtacgctcc tggcccaaaa caatatttcc gtaaaattcg aagctcccca
     3061 ggaacttccg cttgtggaag gcaaccgcag gctgcttacc caggtttttt caaatctgat
     3121 cgccaacgca gaacaatcga tttctgccgc tcgcagcgga ggccgtttgg atatttccgc
     3181 ctgctgcgtg gatggccgta tccgcatcag cttcgccgac gatggtgcgg gcatagatcc
     3241 tgaaatcatc ggcaaaattt tcgatccatt cttcacgacg aagcggcccg gtggagggag
     3301 cggacttggt cttacgattg ctctcgccgt tattaaggag catggcggga ccattgaagt
     3361 tcagtccacc ctaggactgg gcgcaacctt ccatgtgttc ttgccgccct gtgcccaggc
     3421 gtcttcggat tccgcatctt catcctccgc cagccttgcg ccgccgcgct cggctgcgga
     3481 tgttctacag ggtcgcaacg tcgttatcgt cgatgatgaa gaaagcattc gcgaaattgt
     3541 ggccgaagat ctttcaaggc gcgggatgaa ggtccatacg gtggagagtt cggaagcggc
     3601 gctgaactat ctctcaacga acaagtgcga gatcgttatt tgcgatttca atctccccgg
     3661 tatcagcggc gaggctcttt tcgagaagct gcttgcaaaa gagaacggac ctcggccgcg
     3721 gttcgtcttc atgacggggg agcttgtcga ttccggcctt gtgagcaagt ttcgcgaaaa
     3781 aggcgctcgc atcctccaaa aaccgttttc catttccgtc ctggcggcac ttctggcgga
     3841 ttttatgcaa ggagaaacgc ttccttcgag ttagcaggaa tcccgctact tggcgctgtg
     3901 gttcgcctcc cgttgcgaac cggcatcaac gccaaaatcc cagacataaa ggctcgttcc
     3961 ttcgtcgccg accatttccg ccagagcata ttcgccgggc ggtagcggct tgctgagtgt
     4021 gaaacgataa accccgcgcg caatctccca gcgctgcatc gaaatcgtgg ctcccgtggt
     4081 ttgttgctgg ccgaatagct catccacctt ttcgatctgc cggttttcgc cgtgcgcctt
     4141 cgcccgcatc aattccacct cgggtgttcg agcatccgcg gtgcgcatgt aaaattccgg
     4201 ttgctcggtc cgaaggcgaa gtttcgcgtg cattccctga atcgatacat tatgcctcgt
     4261 gggaattatg ggaaccggta tcatcacctg ttcaagaaaa tgccccctgg atagtttcga
     4321 ggttgtctcg gcttgaggaa ccggaagaac ggccttcccg tcgaaggcga aaagcccggc
     4381 gcccggcgga agaaaaattc ggggagccac ctccaggctc gcgtcaatat cgagaggctg
     4441 cgcgagtcgc gccgcttctt ggttgtgcac cttcgcaacg atggccgcct cttgtcgctg
     4501 ctcttgcgcg gcagcttttc cggtggcgtc ccaatcgact aagtctgcgg gaatttcctc
     4561 ccactgcgaa cggtcgaggc tgtaataacg cacccggtcg ccctgcaccg catactcgcg
     4621 aaccagctga aaactgccgt ctttcagcat cagtttctga cccctcggcg ttgtcggcgc
     4681 tttcggggct gggatttgac tattgtggac tgtggattcc tggccggaat ttccagcatc
     4741 ctgagcgctg aggagaggaa ccgcacccac gattatggcg agcagagcac atctggatat
     4801 gtgcaacaac cagcgagacg atcttccacc catgagccta ctcctcgata caattgtaga
     4861 cccaattctg cccgaacgga ttcgcacgaa cagccacatg cgaaagcagc cggctacttg
     4921 tggaccgatc agcggcaagc ccagtcaatt ggggccagca ttcacaacct tgtggcccca
     4981 agttcctaaa agtttcaggg gaaaatgaga ggacggctag tgctggcagc gaggctgaag
     5041 gaccgtaacg atcatcaatt ccgagttggt gaagagccac cgtccggcgt gagtgccagc
     5101 cgtttcagcg gcgttggcca gagttccgag atgcttgttg cgagccaggc cgcgcaaatg
     5161 ctcaatgcag atgcccgtca ggttggcgac ttcatcttcg gtgaaaattt tctggctcga
     5221 cttgattcgg accatcagtc cacctcgaaa cttcttggat actatgttca cagactaact
     5281 gccgacaatt tcgcctgcaa tagccgttcc gtaccaatcg catgggactt tcaattacaa
     5341 cgccccagaa gccttttatc ttccgcaatt taacaaagca ggaatgcgca agctgcgaaa
     5401 aaatcaggcc gcgttctgtt cagaaacgga aagaaaattc acgacacgga atttttgtta
     5461 caaatttttg tctaacgaaa ctcaatggcg gcagctttcg gcgacattta cgatccgaag
     5521 ccgccagcct cctcgaaagc tcgcgcgact tgcaatactc ggccttcggc gaagtgcggc
     5581 ccgaagattt gcatgccgat tggtaagcca gttttgttct tgccgcaagg gaccgagatc
     5641 cctgggacgc ccgccagcga tcccgtaact gtatatatat cagcgagata catctgcagt
     5701 ggatcggctg ttttttcgcc cagtccgaag gctgccgtcg gcgcggtcgg cgtcacgatc
     5761 acatccgcct tgttaaaggc atccgcaaaa tctcgcgcca ccaatgttcg gacgctctgc
     5821 gcccgcaaat aatatgcgtc gtagtagccg gacgagagca cgtaggttcc gagcatgatt
     5881 ctgcgcttca cttccgctcc gaagccgccc tgccgcgtat tgcgatacat ttcggttaac
     5941 gtcgcgccgg gcactcgcag gccgtagcgg acaccgtcat acctcgccaa attggaactt
     6001 gcttcagccg tcgcaaggac gtaatagacg gcggttgcga agtcagtgtg cggcatgcgc
     6061 agcggaaccc gtttgcagcc caggcgctct aggagcgcga tgcccgattg gaccttttct
     6121 ttcacttctg gatcgatacc ttcggcgaag tagtcctctg ggacgccgat tcgcaatccc
     6181 tgaatgggtt tccgggtctc gcttgcatag tccgaaacat caacaggcgc agatgtggaa
     6241 tcgttttcgt catggcccgc gatggcggag attaccgacg ttgcatccgc gatgtttcca
     6301 gcaagcggcc cgattttatc gagggaggac gcaaaggcca ccagcccata tcgcgacacg
     6361 cggccatagg tcggcatcaa cccaggtatt ccgcaaaatg cgccaggctg gcgaattgag
     6421 cctccggtgt cggtgcccag cgctgctacc gcaagcccag cagcgacggc cgcagccgat
     6481 ccaccgctcg atccgccggg gacgcgatcg ggcgcgacgg gattgcgcac cggtccgtag
     6541 gctgaattct cgtttgagcc gcccatggcg aattcatcgc aattggtttt tccgagaagg
     6601 agggctcccg ctttttccag gcgctcgacg gccgtggcgt catacgcggg gacataattc
     6661 tcgagaattt tggacgcaca ggtggtgcga acgccgcgcg tgctgattac gtccttgatc
     6721 gccatcggca cgccggcgag cggcgggaga tcctcgcctc gtgcgaccga tgcatcaatt
     6781 cgatccgcct gggcgtaggc tcgctcgggc gagagggtca gataagcatt cagtttaggg
     6841 ttgcggcgct cgatacgact gtagaactcc gcggccagtt cgcgggtcga gatctttttg
     6901 gcgagcagcg cggcgcgaac gctcgccacg gtccaagggg cggcagcgct catcgcgtga
     6961 tcaccttggg cactcgg
//
LOCUS       DATNDT010000389        12996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2216997, whole genome shotgun sequence.
ACCESSION   DATNDT010000389 DATNDT010000000
VERSION     DATNDT010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2216997"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VN788_12620"
     CDS             <1..444
                     /locus_tag="VN788_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXU21417.1"
                     /translation="IIRAGAKMVNAVANSVVPKITVICGGTFGAGHYAMCGKAYDPRF
                     VFAWPTARYAVMSGESAAGTLVELKVKQLEREGKKLSAADKKELYESIRATYERQTDP
                     RYAAARLWVDAIIDPAHTREALIEALEAAALNPHVAEFKTGVLQT"
     gene            445..1350
                     /locus_tag="VN788_12625"
     CDS             445..1350
                     /locus_tag="VN788_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HXU21418.1"
                     /translation="MGDAVTIVECPRDAWQGIAQQIPTEEKIKYLTRLVSLGFHHIDA
                     VSFVSRKHVPQMADSEAVMQEVSHARLSSGEAPDIIGIVVNQQGMERALATPGVTTVG
                     YPYSISAYFRRANANMSRAESRSLVEQLQREMKAAGRKLVIYISMAFGNPYDEPWGPE
                     IVEDALVWLKDVGVRIVSLADTVGTASPSDVAHLYRAVKDCVAGIDIGVHLHSRPERA
                     AEKVLAAYEAGCRRFDGALTGLGGCPFAGDQLVGNIPTEAVLTALASRGAATGIDTKS
                     LAIACAMTHELREKYAHEPETAKPN"
     gene            1449..2234
                     /locus_tag="VN788_12630"
     CDS             1449..2234
                     /locus_tag="VN788_12630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HXU21419.1"
                     /translation="MEYSTLNFQDDGHGIVTVTLDVPEKRNAISSQMIGELLDALENA
                     EKGSSRVVILSGSGKAFCAGMDLSELQNLAKETPEKNIEDARRLSKMLYRLYSFPKPV
                     IAAVNGPAIAGGSMLATLTDFTLAVPEAKFGYPEVKLGFMPALVVVFLRRQVADRAAR
                     ALLLTGRIIDAAEASRIGLVAEIVPPEKLMERAHKIAAELMAASPTSIARTKKFILGF
                     DDAALQAELEQAVNANADIRSTPDFKEGIASFLEKRSPKWTAE"
     gene            2257..2721
                     /locus_tag="VN788_12635"
     CDS             2257..2721
                     /locus_tag="VN788_12635"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HXU21420.1"
                     /translation="MSDYSETTVRVRYAETDQMGVVYHGNYFTWFEVGRVELCRQLGF
                     EYRQMEIEDDSFIVVAEAQCRYKRPARFDEVLVVRTQLFNSQRRTIRFKYEIVNQASG
                     ELIATGETTHVICDRLGRPKSLPEKYRKYFAEDQTDRSAHSDGKFNPTSRGD"
     gene            2721..3590
                     /locus_tag="VN788_12640"
     CDS             2721..3590
                     /locus_tag="VN788_12640"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU21421.1"
                     /translation="MSAADPAKIERFRDKLREEWTDEQTVAAWRKWNDRMSEFTREAT
                     SAVLEAARLRPGMQVLDLASGVGDPALSIAAAVGPSGHVTATDLGPSMIAFAEELAKK
                     KGLGNIEFRVANVEALAFPDQTFDVVTCRFGVMFFPEQVKAFRECRRVLRPGGRVAFV
                     VWGKREQPFLGTTVGVLMKYVEAVPPDPDAPHAFMFGERGLLQRRLEEAGFSDVREEV
                     RAITGHWKGTPEEYWQQFTEVAAPFRPLIAKLTPETRAKAEAEIFAGLRKLSDGKELS
                     MSLEIVVGTGART"
     gene            3587..5104
                     /gene="hutH"
                     /locus_tag="VN788_12645"
     CDS             3587..5104
                     /gene="hutH"
                     /locus_tag="VN788_12645"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013375994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HXU21422.1"
                     /translation="MTRIQIDGEHLTIEDLHRLVLGDAEAELSSAARAKMHESRVIIE
                     KLIGAGSAVYGVNTGFGKMAATRISPDEIRHLQLNLVRSHACGVGAPLSEPATRAMLA
                     LRANAIAKGFSGVRPILAETLIAMLNRRVHPVLPSQGSAGASGDLAPLAHLAQVAIGE
                     GQAVFEGRTMPGGDAMRAAGIAPLALEAKEGLALLNGTQAMLALLTLALREAEIAADT
                     ADVAIALSLDALRGSPAAFDARIMQVRPHSGAAITARNIERLNRGSEIRESHRSPDVD
                     SRVQDPYSLRCAPQVHGAVRDALAQVRATLAIEINSATDNPLVFADSGDVVSGGNFHG
                     QPLAMAADQMAVALATLGGISERRIEQMTNPQTSMLPAFLVREAGLNSGFMILQVTAA
                     ALASETRALAAPHSVGSIPTSANQEDYVSMGMGAARRLAPMLANLRNILAVELLAASQ
                     GIDFLAPLRPGVEARKAYDIVRSLSKRVDSDRSLAPDIEAVSAAIDSGKFNDLIR"
     gene            complement(5085..6140)
                     /locus_tag="VN788_12650"
     CDS             complement(5085..6140)
                     /locus_tag="VN788_12650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21423.1"
                     /translation="MASWYAQRVRRYEQMRFDQEPARRILPFEWGLEHIGGEARDLNP
                     RKFLDCYVENAVKNSDEWYAAPPADDYALADGVLTFTSAITSPWRENNRVHAQLFPAL
                     RRVNGSGRGPAVIVLAQWNARWEEQQNVCRWLSRLGMTAVKMSLPYHDRRRIPGHPRA
                     DHLVGPNIGLTMQAGRQAVVDVRRTLRWLEQQGYGPLAVLGTSIGSAIGFISMCHEPA
                     LRAGAFLHASTYFGEVVSHGLTTQNVWESLSAGVSREELRHYWSPISPFPYIANLCGA
                     GKRILLITGRYDPTFWPEFTDALLEKLRGEKIGFESLSLPCGHYSMGVAPFSYIAGHR
                     FGRFLQRSLAGTNGSGR"
     gene            complement(6159..6773)
                     /gene="folE"
                     /locus_tag="VN788_12655"
     CDS             complement(6159..6773)
                     /gene="folE"
                     /locus_tag="VN788_12655"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HXU21424.1"
                     /translation="MSETKVVDGDQIIIPGADSAGKIAELVRAMIVQLGEDPSREGLR
                     RTPERFEKALRYLTSGYRQDPEKLLNGAMFSVGYDEMVVVKDIEIYSLCEHHLLPFFG
                     KCHVAYIPNKKVVGLSKIARLVNMYARRLQIQERLTSQIAKTIEDKLNPQGVGVIVEA
                     RHLCMVMRGVEKQNSAAVTSAMLGVFRENKQTRDEFLSLVRGAK"
     gene            complement(6778..7230)
                     /locus_tag="VN788_12660"
     CDS             complement(6778..7230)
                     /locus_tag="VN788_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXU21425.1"
                     /translation="MSNPLKLSLTRRYRFSASHRLHRANLSEEENLRLYGKCSNLHGH
                     GHNYVLEVTITGPVDPATGMIANLGELDPFVEREVIEPFDYKYLNEEVPEFAANVPTT
                     ENVCREIYRRLKKFPAARIARVRIEETSKNTFEYDGDSNGVEGLRHEG"
     gene            complement(7227..7673)
                     /locus_tag="VN788_12665"
     CDS             complement(7227..7673)
                     /locus_tag="VN788_12665"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HXU21426.1"
                     /translation="MIKITRKIEFSAAHYYHNPNFSAEENRRVFGKCNNPHGHGHNYV
                     LEVTVAGEPDPDTGMVLDLKELKDILQCEVADRMDHRHLNYEVPELAGQIPTCENIAA
                     VIWRLIEPKITKGKLDRVRLYESPDLFADCTAERNGAGGSEVGGNR"
     gene            complement(7670..8698)
                     /locus_tag="VN788_12670"
     CDS             complement(7670..8698)
                     /locus_tag="VN788_12670"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXU21427.1"
                     /translation="MISAIIPARNEEAAIARAVESVAAQPEVAEVIVVDDQSSDGTAA
                     VLRDLGERIPKLKVLRTGELPRGWVGKNHAAAMGAGAAAGDWRLFTDADTFHLPGSAR
                     GALCDATDHDAALVSYSPEQEMVTFWERALIAFVYCRLAARFSFARINDPRQPDAAAN
                     GQFLMIRRDAYDAVGGHAAVAAEILEDVALARRVKQRGFGIYFAAPSGVVRTRMYRSL
                     RAMWQGWTKNLYPLMGASFPSLLFELLEASPIVELVALLAILALYLTGFRSDLLSPAA
                     GIVAGMLLSRNVLHLAALHRNRYPISYIKYYFTGAALYSAALVESWWKSTHGGVVWKG
                     RTYPAGAR"
     gene            8682..9311
                     /locus_tag="VN788_12675"
     CDS             8682..9311
                     /locus_tag="VN788_12675"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXU21428.1"
                     /translation="MMAEIKVFAIRSEGLLKYRDRVEDASIIGPLDTGLKRKLNTGLV
                     LAFVAGLLVLFARPEYQQGEPSLRGRPEKDFALTLNGKATRLSDLRGKVVLLNFWASW
                     CPPCRDEAPALNQLQRRIAPSGGMVLGVNAGVDDSQAGYEDFLKTYNIDFPTYLDPSK
                     RIALSYGTTMYPETYVINRKGRIDRKIIGPQDWTSPTMMAYLDSVLKEP"
     gene            9372..10007
                     /locus_tag="VN788_12680"
     CDS             9372..10007
                     /locus_tag="VN788_12680"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HXU21429.1"
                     /translation="MVDFAVVPERTALLNVDLQNCFVEGSPIAAPDGLLILERVNRLA
                     AACRKTGILVIHASVVIRPDHSNLGTLAEFNPFAKEGILDKGAHSAALHKGLVVEPSD
                     ILLDKPRFGAFHGTDLELILRNRGIDTVIISGIATNICCETSAREAAVRDFRVIFLSD
                     GTATTPMGGLSAAELQKATCATLGYLFAQILTVNEIIRKIEGVARAAASRS"
     gene            10215..12683
                     /locus_tag="VN788_12685"
     CDS             10215..12683
                     /locus_tag="VN788_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HXU21430.1"
                     /translation="MEKDVVCGMTIDPERAPAKVEHEGKTYYFCGASCAAKFRAGSEK
                     FLRAKPADSGRAMPLVQLGSAKPITIAKAADAPLPKLAVYVCPMDPEVREDRPGPCPK
                     CGMALEADVPSAQAARIEYTCPMHPEIVRDQPGSCPICGMALEPRTAVVGEEENPELL
                     AMTRRFWVSAALAVPVLALGMSDMIPGHPVQRVLSVRAIEWIQLLLATPVVVWGGWPF
                     FQRGWASLVNRSLNMFTLIALGTGTAYVYSVIAVLFPGIFPLSFRLAGGQVPVYFEAA
                     AMITALVLLGQVLELRARSRTSAAIRALLKLSPKIARLVRTDGTEIDIPVEHIAVGDV
                     LRVRPGEKIPIDGIAIDGSSSVDESLVTGEPIPIEKTSGSRVTGSTVNGTGTLLIRAE
                     RVGNETLLAQIVRMVSEAQRSRAPVQQLADRVSAYFVPAVVLVAILTFILWSLFGPAP
                     RMAHALLNAVAVLIIACPCALGLATPMAIMVGTGRGALAGVLIKNAEALEILEKVDTL
                     VVDKTGTLTEGRPRVVSVIAANGFDEVQVLRASATLERASEHPLAAAILTAAKERDIA
                     SGNVSAFRSVTGKGVTGVADGQAVALGNRALLLELGISPGNLDERGTLLESDGQTVMF
                     VAIAAKIAGIIGVADPVKATTPEAIERLHRERIRIVMITGDSRATAEAVARRLGIDQI
                     HAGVLPDKKGEIIKQLQAERHVVAMAGDGVNDAPALVQADVGIAMGTGTDVAMESAGI
                     TLLRGDLRGIARARALSQATMRNIRQNLFFAFLYNSAGVPIAAGVLYPFLGLLLSPIF
                     ASAAMTFSSVSVIGNALRLRNVHL"
     gene            12652..12969
                     /locus_tag="VN788_12690"
     CDS             12652..12969
                     /locus_tag="VN788_12690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21431.1"
                     /translation="MRFGSETFIYSYRLGTHNIGALRGNAILTLMTFMSVSYELQDKL
                     KPEQFRALGMFANTYGLQRFRVDEKSNILHFDYDASRLRENVVEHVLREARIPVLRRV
                     AQA"
BASE COUNT         2662 a         3893 c         3791 g         2650 t
ORIGIN      
        1 atcattcgcg cgggcgcaaa gatggtcaac gcggttgcga acagcgtggt gcccaagatc
       61 acggtgatct gtggcggcac cttcggcgcc gggcattatg cgatgtgcgg caaggcctac
      121 gatccacggt ttgttttcgc ctggccaacg gcgcgttacg cggtaatgag cggcgaatcc
      181 gccgctggca cgctagtcga actgaaggtc aaacagcttg aacgcgaggg gaagaagctg
      241 agcgccgcag acaagaagga gctatacgaa tccatacgcg cgacctacga gcgacaaacc
      301 gatccgcgct atgctgcggc gcgattgtgg gtcgatgcca tcatcgatcc ggcacacacg
      361 cgtgaggcgt tgatcgaagc gttggaagcc gcggcattga atccgcacgt tgccgaattc
      421 aaaacgggag ttttgcaaac ctaaatgggc gacgcggtca ccattgtcga gtgcccgcgg
      481 gatgcgtggc aaggcatcgc gcagcagatt ccgaccgagg aaaaaataaa atatctgacg
      541 cggctggtat cgctgggctt tcaccacatt gatgccgtca gctttgtctc acgcaagcac
      601 gtcccgcaga tggccgatag tgaagcggta atgcaagaag tgagccacgc caggctgtcg
      661 tcgggtgagg cgccggatat tatcggcatc gttgtcaatc agcaaggaat ggaacgtgcg
      721 cttgcaacgc cgggcgtaac gacggtcggt tacccgtatt cgatctcggc atatttccgt
      781 cgcgcgaatg caaacatgtc ccgcgcggaa tcgcgttcac tggtggaaca gcttcagcgc
      841 gaaatgaaag cggcgggacg gaagctggtg atttacattt ccatggcgtt tggaaatccg
      901 tacgacgagc cttggggccc tgaaatcgtg gaggacgcgc tggtgtggct aaaagatgtg
      961 ggagttcgaa tcgtatcgct tgcggatacg gtgggcacgg cgtctccatc tgacgtggcg
     1021 catctgtatc gcgccgtgaa agactgcgtg gcgggaatcg atatcggcgt gcatctccac
     1081 agccggccgg aacgtgccgc tgaaaaagtg ctggccgctt acgaagcggg atgcaggcgg
     1141 tttgacgggg cccttacggg gcttggcggg tgtccattcg cgggcgacca gttggtcggc
     1201 aatattccca ccgaagcggt gctgaccgcg ctcgcctcgc gcggcgcggc cactgggatc
     1261 gacacgaagt cgctcgccat cgcctgcgca atgacgcatg aactccgcga gaagtacgcg
     1321 cacgagcctg aaacggcgaa accaaactga attcccttcc ggtagccgct acgcgattca
     1381 caacctttga tttttgccgc gttcggtgct tatctgtgtc cgacagtttc cgctagagag
     1441 gtcatcccat ggagtattcg acgctcaatt ttcaggacga cgggcatgga atcgtcaccg
     1501 tcacgctgga tgtgccggag aagcgcaacg ccatctcatc gcaaatgatc ggtgagttgc
     1561 ttgatgcgct ggaaaacgcc gaaaagggtt cgtctcgcgt cgtgatcctc tcaggatctg
     1621 gcaaagcctt ctgcgctgga atggatctga gcgagttgca gaatctggcg aaggagactc
     1681 cggaaaagaa tatcgaggac gcacgccgtc tctcaaaaat gctgtaccgc ctatacagct
     1741 tcccaaagcc ggtgatcgcg gcggtgaatg gcccggcgat cgccggtggt tccatgctcg
     1801 cgacactaac tgacttcaca ctggctgttc cggaagcgaa gttcggctat ccggaggtaa
     1861 agctcgggtt catgccggcc ctggtggtgg ttttcctgcg ccgccaagtt gccgatcgcg
     1921 ccgcccgcgc gctgctgctc accggaagaa ttatcgacgc ggcggaggcg tcccggatcg
     1981 gtttggtggc ggagatcgtg ccgcccgaga aactgatgga gcgcgcacat aaaattgcag
     2041 ccgaattgat ggcggcaagc ccaacctcta ttgcgaggac gaagaagttc atactcggtt
     2101 tcgacgatgc cgcattgcaa gctgagctgg aacaggccgt caatgcgaat gccgatatcc
     2161 gctcgacacc tgatttcaag gaaggcattg cgtcgttcct cgaaaagcgt tctccgaaat
     2221 ggacggccga gtaaaccggc gcgcgcgaca cagaccatga gcgattattc ggaaacaacc
     2281 gttcgcgtgc gctatgccga gacggaccaa atgggggtgg tctatcacgg aaattacttc
     2341 acgtggtttg aagtgggacg cgtcgaactc tgccgacaac tcggattcga atacaggcag
     2401 atggaaattg aagatgatag cttcatcgtg gtagccgagg cgcagtgccg ttacaagcgc
     2461 ccggcgcgct tcgatgaagt gctcgtggtt cgcacgcagc tcttcaattc acagcggcgc
     2521 acgattcgct tcaagtacga gattgtgaat caggcctcgg gcgagctcat cgccacaggc
     2581 gaaacgacgc acgtgatctg cgacaggctg ggccggccga aatcattgcc ggaaaagtac
     2641 cgcaagtatt tcgcggaaga ccagaccgac cgctccgcgc attcagatgg aaaattcaat
     2701 cctacttcga gaggtgactg atgagcgccg ccgatcccgc caaaatcgaa agattccgcg
     2761 acaagcttcg ggaagaatgg accgacgaac aaacggttgc cgcctggcgg aaatggaatg
     2821 accggatgag cgagttcacg cgcgaggcaa cctcggccgt tcttgaagcg gccaggctgc
     2881 gaccgggaat gcaagtgctc gatctggcaa gcggcgtggg cgatcccgcg ctgtcgatcg
     2941 cggcagcggt tgggccatcc ggccacgtga cggcgaccga tcttggtccc agcatgatcg
     3001 ctttcgcgga ggaactggcg aaaaagaagg gactcggtaa tatcgaattt cgcgtagcga
     3061 atgtcgaggc gctggctttc cccgatcaga cgttcgatgt ggttacttgc cgcttcggtg
     3121 tcatgttctt cccggagcag gtgaaagcgt ttcgcgaatg ccgacgcgtc ctgaggcccg
     3181 gcgggcgcgt ggcattcgtc gtatggggca aacgcgagca gccatttctg ggcacgacgg
     3241 tcggcgtact gatgaagtac gtcgaagcag tgccacccga tccggacgcg ccccatgcat
     3301 tcatgttcgg tgagcgcggc ctgctgcagc gccggctcga agaggcggga ttttcggatg
     3361 tgcgagagga agtacgcgct atcaccggac actggaaagg gacgccggaa gagtactggc
     3421 agcagtttac agaagtagcc gcgccatttc gtccgctgat cgcgaagctc acgccggaaa
     3481 ctcgcgcgaa agcggaggct gagatttttg caggactgcg gaagctctcg gatggcaaag
     3541 aattgtcgat gtcgctggaa atcgtggtgg gcacaggcgc gcggacttga cgcggattca
     3601 aatcgacggc gaacatctga ccattgagga cctgcatcgt cttgtgctcg gcgatgcgga
     3661 agctgagcta tcgtcagcgg cgcgagcgaa aatgcacgag tcgcgcgtga taatcgaaaa
     3721 actgattgga gctggcagcg cggtttacgg cgtgaacaca ggcttcggca agatggccgc
     3781 cacgcgaatc tcgccagatg aaattcgcca ccttcagctc aatctggtgc gcagccacgc
     3841 ctgcggtgtc ggcgctccgc tgagcgagcc ggccacgcgc gccatgctcg ctttacgcgc
     3901 gaacgccatc gccaaaggtt tttccggagt gcgcccgatc ttggcggaga ctttaatcgc
     3961 gatgctcaat cggcgcgtgc atcccgtgct cccctcgcaa ggaagcgccg gcgcttcagg
     4021 cgatcttgcg ccgctcgcgc atcttgcgca ggtggcgatt ggcgaagggc aagcggtgtt
     4081 tgagggccga acgatgcccg gtggtgatgc gatgcgcgct gccggaatcg ccccgctcgc
     4141 gctcgaagcc aaggaaggcc tggcgctgct caacggcaca caagcaatgc tggcactcct
     4201 gacgcttgcg ttgcgtgaag cggaaattgc ggcagatacc gcggacgtag cgatagcgct
     4261 ttccctcgac gcgctgcgcg gcagtccggc ggcattcgac gcgcgcatca tgcaggtgcg
     4321 gccgcacagc ggcgccgcga tcacagcgcg taacatagag cggctgaatc gcggcagcga
     4381 aatccgcgag tctcatcgtt cgcctgatgt ggactcgcgc gtgcaggacc cctacagcct
     4441 gcgctgcgcg ccccaggtgc acggcgccgt tcgcgacgcg ctggcgcagg ttcgcgctac
     4501 gctcgcaatc gaaatcaaca gcgccactga taatcccctc gttttcgccg acagcggcga
     4561 cgtcgtcagc ggcggcaatt ttcacggcca gccgctggcg atggccgccg atcagatggc
     4621 cgttgctctt gcaacgctgg gcggaatttc cgagcgccgc atcgagcaga tgaccaatcc
     4681 gcagaccagc atgctgccgg cgtttttggt gcgcgaagca gggctcaatt cgggattcat
     4741 gattttgcag gttaccgccg cggccctcgc gagcgagaca cgtgcacttg cggcgcctca
     4801 ctcggttggc tcaattccca cttctgctaa ccaggaagac tacgtgtcga tgggaatggg
     4861 ggcggcgcgc cgtctcgcac ccatgctcgc gaatctgcgg aacatcctcg cggtcgaact
     4921 cctcgcggcc tcccagggaa ttgactttct cgcgccactg cggccggggg ttgaagcgcg
     4981 caaggcctac gatatcgtgc ggtcgctatc gaagcgcgtg gactccgacc gttcgcttgc
     5041 gcccgatatt gaagcagtgt ccgccgccat cgacagcgga aaattcaacg acctgatccg
     5101 ttagttcccg caagcgagcg ctgcaaaaat ctgccgaagc gatggcccgc gatgtagctg
     5161 aacggtgcga ctcccatcga gtaatgtccg cacggcagcg agagactttc gaatccaatt
     5221 ttctcgccgc gcaatttttc taagagcgca tcggtaaatt caggccaaaa agttggatcg
     5281 tagcgcccgg taatcagcaa aatcctcttc cccgccccgc acagattcgc aatgtacgga
     5341 aatgggctga taggcgacca gtaatggcgc agctcctcgc gcgagacgcc cgcactcagc
     5401 gattcccaca cattttgcgt ggtcaatccg tggctcacca cttcgccgaa gtatgtggaa
     5461 gcatgcagaa acgcgccggc gcgcagtgcg ggttcgtggc acatagagat gaacccaatc
     5521 gcggagccaa tgcttgtccc gagcacggcc agcggaccat agccctgctg ctcaagccaa
     5581 cgcagtgttc gacgaacatc cacgaccgcc tgccgccccg cttgcatggt gagcccaatg
     5641 ttaggcccga ccaaatggtc ggcgcgcgga tggccaggaa ttctgcggcg atcgtgatac
     5701 ggcaggctca tcttgaccgc cgtcattccc aggcggctta gccaccggca aacattctgt
     5761 tgctcttccc agcgcgcatt ccattgcgcg aggacgatca ccgccgggcc gcgacctgaa
     5821 ccgttcacgc ggcgcaacgc cggaaaaagc tgcgcgtgaa cgcgattatt ttcgcgccac
     5881 ggtgaagtga tcgcgctggt gaacgtgagg acgccatcgg caagcgcgta atcatccgcg
     5941 ggcggcgcgg cataccattc gtcgctattc ttgaccgcgt tttccacata acaatcgaga
     6001 aattttcgcg gattcaaatc gcgcgcttcg ccgccaatgt gttccaggcc ccactcaaag
     6061 ggcagaatgc ggcgcgccgg ctcctgatcg aagcgcatct gttcgtaccg ccggactctt
     6121 tgtgcgtacc aggaagccat caggcgagca tttgaatatt atttggctcc gcgaaccagg
     6181 gagagaaatt cgtcgcgggt ctgcttattt tcgcggaaga cgcccagcat cgcgctcgtc
     6241 accgccgctg agttctgctt ttccacgccg cgcatcacca tgcaaagatg tcgcgcctcg
     6301 acgatcacgc ccacgccttg cgggttcagc ttgtcttcaa tggtcttcgc gatttggctg
     6361 gtcagccgct cttgaatctg cagccgccgc gcgtacatat tcactaaccg cgcgattttg
     6421 ctcaatccca ccactttttt atttggaatg tatgccacgt ggcacttccc gaaaaaagga
     6481 agcagatgat gttcgcaaag gctgtagatt tcgatgtctt tcaccaccac catttcgtcg
     6541 tatccgacgc tgaacatcgc gccattcaga agtttctccg gatcctggcg gtagccgctg
     6601 gtcaaatagc gtagcgcctt ttcgaagcgc tccggagtgc gtcgcagccc ttcgcggctg
     6661 ggatcttctc cgagttggac aatcatcgcc cgcaccagtt cagcgatttt cccggcgctg
     6721 tccgcgccgg gaatgatgat ctggtccccg tcgaccacct tggtttcgct catcgattta
     6781 gccctcatgg cgcagccctt ccacgccgtt ggagtcgccg tcatattcga aggtattttt
     6841 gctggtttct tcaatccgca cccgtgcaat gcgtgcggcg ggaaatttct tcagccggcg
     6901 gtatatttcg cggcagacgt tttcggtagt cggaacgttg gccgcaaatt caggcacttc
     6961 ctcgttcagg tacttataat cgaacggctc aatcacctcg cgctcgacaa atgggtcaag
     7021 ctctcccaga ttcgcgatca tccccgtagc cggatcgacg ggacccgtga tggtgacttc
     7081 aagcacataa ttatgcccgt gaccatgcag attgctgcat tttccgtaca agcgaaggtt
     7141 ttcctcctcg ctcagattcg cacgatgcag ccggtgcgac gccgaaaacc gatagcggcg
     7201 cgtcagcgaa agctttagag gattgctcac cgattgcctc ccacctccga cccgcctgcg
     7261 ccgttgcgtt ccgccgtgca atccgcgaat aaatcgggag actcataaag ccgcacgcga
     7321 tcgagttttc ccttggtgat tttcggctcg atcaaacgcc agatcactgc ggcgatattt
     7381 tcacaggtcg ggatctgccc cgcgagctcc ggcacttcat aattcaagtg acgatgatcc
     7441 atgcggtcag ccacttcgca ctgcaaaata tccttcaact ctttcagatc caagaccatt
     7501 cccgtgtccg gatcgggctc gcctgccacc gtcacttcca gaacgtagtt gtgtccgtgg
     7561 ccatgcggat tgttgcactt gccgaagacg cggcggttct cctcggcgga aaaattcgga
     7621 ttgtgataat agtgcgccgc cgaaaattcg attttgcgcg tgattttgat catcgagcgc
     7681 ccgccggata cgttcgcccc ttccagacca ccccgccgtg cgtgctcttc caccacgact
     7741 caacaagcgc cgcgctataa agcgccgcac cggtaaaata gtatttgatg taagaaatcg
     7801 ggtagcgatt ccgatgaagc gctgccaagt gcagaacgtt cctgctgagc agcattccag
     7861 ccacaatgcc cgccgccggc gacaacagat cgcttcgaaa gccggtgagg taaagtgcca
     7921 ggatcgccag cagcgccacc agctcgacga tcggagaagc ctccagcaat tcgaacaaaa
     7981 gcgacgggaa actcgcgccc atcagcgggt aaagattttt ggtccagcct tgccacatgg
     8041 ccctgagcga tcgatacatc cgcgtgcgca ccactccgct cggagcggca aagtaaattc
     8101 cgaagcctcg ctgcttcacg cgccgcgcca aggcaacatc ttccagaatt tcggcggcaa
     8161 ccgctgcgtg tcctcccacc gcatcgtacg catcgcggcg aatcatcagg aattgcccat
     8221 tggctgcggc gtccggctgc ctcggatcgt tgatgcgcgc aaacgaaaat cttgccgcca
     8281 gccggcaata gacaaatgca atcagcgccc gctcccagaa tgtcaccatt tcctgttcgg
     8341 gcgagtatga caccagggcc gcatcatgat cggtggcgtc gcaaagcgcc ccccgcgccg
     8401 atcccggcaa atgaaacgta tcggcatccg taaagagccg ccaatcgccg gcagccgcgc
     8461 cggcgcccat ggccgcggcg tgattcttgc caacccaccc gcgtggaagt tcgcctgtgc
     8521 gaagcacctt cagcttcggt attcgctcgc ccaaatcgcg caaaaccgcg gctgtgccgt
     8581 cgctcgattg gtcgtccacc acaatcactt ctgctacctc aggctgggcc gctacggatt
     8641 cgacggcgcg tgcaatcgcg gcttcttcgt ttcgcgcggg aatgatggct gagatcaagg
     8701 tttttgcgat ccgatccgaa gggctgctaa aatatcgcga tcgagttgaa gatgcttcta
     8761 ttataggacc cttggatact ggattaaaac gcaaactgaa cacaggcctc gttctagcct
     8821 tcgtcgcggg cctgctcgtg ctattcgccc gtccggagta tcagcagggc gagccgagcc
     8881 ttcgaggacg tccggaaaag gatttcgctc tcaccttgaa cggcaaagcc actcgccttt
     8941 cggatttgcg cggaaaagtt gtcctgctga acttctgggc cagttggtgc cctccctgtc
     9001 gcgacgaagc gcctgcgcta aaccagttgc aacgccggat cgccccatca ggtggcatgg
     9061 ttctcggagt caatgcgggc gtggacgaca gccaggcggg ctacgaagat tttctgaaaa
     9121 catataatat cgacttcccc acctatctcg atccctcaaa acgcatcgcc ctttcctacg
     9181 gcaccaccat gtatccagaa acgtacgtaa tcaatcgcaa gggccgcatc gaccgcaaga
     9241 tcatcggtcc gcaggattgg accagcccca cgatgatggc ctacctggat tccgtgctaa
     9301 aggaacccta aaagcttctt ctcgacctgc ccccaacctg acggtacaat ctcccctttc
     9361 ggaggctcgc catggtggac ttcgccgttg ttcccgagcg tacggcccta ttgaacgtcg
     9421 atttgcaaaa ttgcttcgta gaaggctctc cgattgccgc gcccgacggt ttgctcattc
     9481 ttgagcgggt gaatcgtcta gctgccgcct gccgcaaaac cggaattctc gtcatccacg
     9541 ccagtgtcgt cattcgaccg gatcactcca atcttgggac tcttgcggag ttcaatccgt
     9601 tcgcgaagga aggcattctc gacaagggag cacattcagc agcgctgcac aaaggcctcg
     9661 tcgttgaacc cagcgacatc cttctcgata agccgcgctt cggcgcattc catggcaccg
     9721 atctcgaatt gatccttcgc aaccgtggca tcgacaccgt catcatcagc ggtatcgcca
     9781 ccaacatctg ttgcgagacc agcgctcgcg aggccgctgt tcgcgatttt cgtgtgattt
     9841 tcctgagcga cggaacagcg acaaccccaa tgggcggcct ttctgcggcg gaacttcaaa
     9901 aggccacctg cgctaccctc ggctacctgt tcgcgcagat cctcacggtt aacgaaataa
     9961 ttcgcaaaat cgaaggcgtg gcacgcgccg ctgcgtcgcg atcgtagcga accgactcta
    10021 tatgcttggc tgcgtatcgt cagcctcaag tgaaggcccc cgcatgtgga gtgcgggagc
    10081 ttgctcccgt ccgtttgccg ttcgggcttg gccggacgtg ctcctcgcac ctgctcgccg
    10141 ccgacctcac cgttttacct ctgcgttcgc gtcttcttgc ttcaggtact ataaaagtcc
    10201 agcatactgg gctcatggaa aaagatgtcg tttgcggaat gacgattgat ccggagcgcg
    10261 ccccggcaaa ggttgaacac gagggaaaga cgtattactt ctgcggcgct tcgtgtgcag
    10321 caaaattccg cgccggcagc gaaaagttcc tccgcgcaaa accggccgat tccggccgcg
    10381 caatgccgct cgttcaattg ggttccgcga aacctataac aattgccaaa gccgcggacg
    10441 ctccgctacc aaagcttgcc gtctacgttt gtccgatgga ccccgaggtg cgcgaggatc
    10501 gcccggggcc ttgccctaaa tgcggcatgg cgctcgaagc cgacgttccc tccgcgcagg
    10561 ccgctcgaat cgaatacacc tgtcccatgc atccggaaat cgttcgcgac cagcctggct
    10621 cctgtccgat ttgcggcatg gcgcttgagc cacgaaccgc tgtggtcggc gaggaggaaa
    10681 atccggagct tctcgcgatg acgcggcgat tttgggtcag cgcggcgctt gcggtcccag
    10741 ttcttgcgct gggaatgtcg gacatgattc ctggccatcc cgtgcagcgc gtgctatcgg
    10801 tgcgcgcgat tgaatggatt cagttgttgc tcgcaacgcc ggtcgtcgtt tggggtggct
    10861 ggcccttctt ccagcgagga tgggcttcgc tcgtgaatcg cagcttgaat atgttcactc
    10921 tgatcgcgct cggcaccggc actgcgtacg tctacagcgt cattgcggtt ctctttccag
    10981 gcattttccc gctttcattt cgattggccg gcgggcaggt gcccgtgtat ttcgaagcgg
    11041 cggcgatgat taccgcgctg gttctgctgg gacaagtgct cgaattgcgc gcgcgcagcc
    11101 ggacgtcggc ggcgatccgt gcactgctga agctctcgcc gaaaattgcc cgcctggttc
    11161 gcaccgacgg cacagaaatt gacattccgg tagagcacat cgccgtcggc gacgtgctcc
    11221 gcgtgcggcc cggcgaaaaa atccccatcg atggaatcgc aatcgacgga tcgagttcgg
    11281 ttgacgagtc gctggtcacc ggcgaaccaa ttcctatcga gaaaaccagc ggcagtcgcg
    11341 taaccggcag caccgtgaac ggcacgggaa cgctgctgat ccgcgccgaa cgcgtgggca
    11401 acgaaaccct tctcgcgcaa atcgtccgca tggtcagcga ggcgcagcgt agccgcgcgc
    11461 cggtgcagca gttggcggac cgcgtatccg catacttcgt ccccgcagtc gtgctcgtcg
    11521 ccattctcac attcattttg tggtctctgt ttggcccggc gccccgaatg gcgcatgccc
    11581 tcctcaatgc cgtagccgtg ctcataattg cctgcccttg tgcgctggga cttgccactc
    11641 cgatggccat tatggtggga acaggacgtg gcgcgctggc tggcgtgctg atcaagaatg
    11701 ccgaggccct cgaaatcttg gaaaaagtcg atactctcgt cgtggataag accggcacgc
    11761 tcacggaagg gcgcccgcgg gtcgtgtccg tcattgctgc aaatggtttt gacgaggtgc
    11821 aagttctgcg cgcgagcgcc acgctcgaac gcgcgagtga acatccgctc gctgccgcga
    11881 ttcttacggc ggctaaagaa cgcgacatcg catcgggaaa tgtctcggct tttcgatcag
    11941 tcaccggcaa aggtgtaacg ggagtagcag atgggcaagc cgttgcgctg gggaatcgcg
    12001 ctctgttgct tgagttagga atatcgccag gcaatctcga cgaacgcggc acactgttgg
    12061 aatccgatgg tcaaacggtg atgttcgtcg ccatcgccgc aaaaatcgcc ggaatcatcg
    12121 gcgtcgctga tccagtaaag gccacaacac ccgaggccat cgaacgcttg catcgcgaac
    12181 gcattcgaat cgtgatgatc acaggcgaca gccgcgcaac cgccgaagcg gtggcgcgca
    12241 ggctcggcat cgatcaaatt cacgccggcg tgctgcccga taaaaagggc gaaattatca
    12301 agcaactgca ggccgagcgc catgttgtcg ctatggcggg cgacggagtg aatgacgctc
    12361 ccgcgctcgt gcaagccgat gtcggcatcg ccatgggaac gggcaccgac gtggccatgg
    12421 aaagcgccgg tatcactctg ctccgcggtg atttgcgcgg aattgcgcgg gcgcgcgccc
    12481 tcagccaggc caccatgcgc aacatccgcc aaaatctttt ctttgcgttt ttgtacaatt
    12541 cggcgggcgt tcccatcgcg gccggcgtcc tctacccatt tcttgggctg ctactcagtc
    12601 cgatttttgc aagcgcggct atgaccttca gctccgtttc ggtgatcggc aatgcgcttc
    12661 ggctccgaaa cgttcattta tagctaccgg ctcggcacgc acaacatcgg cgcgctacgc
    12721 ggcaatgcta tactgacact tatgacattt atgagcgtga gctacgaatt gcaggacaag
    12781 ctgaagccgg agcagtttcg cgccctcggc atgttcgcga atacctatgg cttgcagcga
    12841 tttcgcgtcg atgagaaatc caacatcctg cacttcgatt acgatgcctc gcggctgcgc
    12901 gaaaacgtgg tcgagcacgt cctgcgcgag gcgagaattc ccgtgctgcg gcgcgttgcg
    12961 caagcctgat ttttcataac gaatgtttgg gtagtg
//
LOCUS       DATNDT010000390         7084 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_868718, whole genome shotgun sequence.
ACCESSION   DATNDT010000390 DATNDT010000000
VERSION     DATNDT010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7084
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868718"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..700)
                     /locus_tag="VN788_12695"
     CDS             complement(134..700)
                     /locus_tag="VN788_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008478115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HXU21432.1"
                     /translation="MPETKLPGIKIAILATDGFEESELMQPRAALEGAGAKTVLISPK
                     DNEIQGMQHHDKSKQVDVDLPLAEADAGDYDAVLLPGGALNADALRVEKAAQEFVREM
                     DRARKPIAVICHGPWLLVSAGLVKGRTLTSYHTIQDDIRNAGGNWRDEEVVRDGNWVS
                     SRQPSDIPAFNREMLALFAQAKAKAKTA"
     gene            complement(813..2000)
                     /locus_tag="VN788_12700"
     CDS             complement(813..2000)
                     /locus_tag="VN788_12700"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HXU21433.1"
                     /translation="MAENKSRFERVIWIVLDSVGIGELPDAADYGDVGRDTLGHIARS
                     RPLQIPNLVRLGLASIKPLEHLAPPARPEGSFGKGATVSPGKDTTTGHWEMAGIWLEQ
                     AFPVYPHGFPKEVIAAFEGRIGRRTLGNKPASGTEIIKELGEEHVRTGFPIVYTSGDS
                     VFQIAAHEDVIPIAELYRMCEIARTLLDGPNRVGRVIARPFTGTPGNFRRTERRHDYA
                     VEPPRPMLLDVLAERKVPVFGVGKIHDIYNGRGVRDYDTTRNNADGMAKLTESLSRQS
                     RGLIFANLVDFDMLYGHRKDVEGFASSLEEFDALLGPFLSSLRASDLLLITADHGCDP
                     DPVNPTTDHSREYVPILAYSPRASGGTNLGTRSTLADMGQTVAENFGASIPHGTSFLE
                     AIR"
     gene            complement(2096..3064)
                     /locus_tag="VN788_12705"
     CDS             complement(2096..3064)
                     /locus_tag="VN788_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclease"
                     /protein_id="HXU21434.1"
                     /translation="MNATGKTLSRAFASPSWIVVAIMMLLIGEVPAFGWGNRGHTDIN
                     FVAASKIPKSMPLFFRTRAAVARIAYLGPEPDRWRGASEYALNNSQSPDHFIDLERVE
                     GLGALPKGRYEFYKMLYAKRAATQVHPDDYLPENVGLQPYITMEVFERLKVAFRDYRS
                     LKQAGKDTGAVEQDAIFYAGWLGHYVADGSQPLHTTIDYDGWVGANPDGFTTQRGIHW
                     RFENDFVNRTITAKDFAAMAGAPHQLDDVFNDYLKYLWVSHGFVKQIYEFDKSGSLNG
                     SGTPEAVKFTEQRLAAGSQMLLDLWYTAWLESAEPAPGGANPVPAK"
     gene            3471..4718
                     /locus_tag="VN788_12710"
     CDS             3471..4718
                     /locus_tag="VN788_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21435.1"
                     /translation="MQDMTGVAPNEVQETGIRRGLLEDIALKTLYLNGEMSLTELSKH
                     MCLGGGVIDEIFQFFRKEQLCEVKGMVGGSHRIVASAQGRSRAAGLLAMSQYTGPAPV
                     SLADYRLRVNAQTIQQTGIQPSDLKRALHPLILDVGFVARLGTAVVSGTSIFLYGPSG
                     TGKTSIAACIPAIYSDNVWIPYAVEVDGQIISVYDPGVHRESGEPVSEEADKRWVLCQ
                     RPRILAGGELSSEMLDIQLSSNRFYTAPLQMKANNGVLILDDFGRQRMRADELLNRWM
                     TPLDRRVDFLTLPGGRKFEIPFDVCVVFSTNLDPRELGDEAFVRRIPNKIHVPHATRA
                     QFMEIFRAECTMRLLECEPGLPEHAVEVITQELKQPLSQCYPRDLINQIFWSASYLGV
                     EPRLTRDVLNQACHDYFVSTAKS"
     gene            4926..6122
                     /locus_tag="VN788_12715"
     CDS             4926..6122
                     /locus_tag="VN788_12715"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21436.1"
                     /translation="MNRFNLRLFGCILVCLTLGTFSSLFAQDLTIRNARIIVGNGTVI
                     DNGSIVIRGGKIVSAAAGRPAATAGKVIDAKGMSAMPGYIDAHKHINNFKADQMQDLL
                     EAGYTTVLSGGGSAESVAKLRDQIESGAMNGPRLIVSGPVNVRMGADIARQQVDDLAA
                     KGVKWTGEIALTPEPGPSAMEIEGLKAAVDEGAKKGVTIQVHAVSVPAMMAAIDAGVR
                     HLVHVPNKGWVTVADAQKLTSTGTHILATIGFGAPVFGVFADDNNPRFRNGKPWPASI
                     AGADLLDGVAVGQEIGYTMVNSRTIWDAGGILGYCTDDGYEARAGLEHELKTYNVMFS
                     MKDLVKLMGPNTADYLGMSDQLGTLEPGKLADIVLQDGNPMEGYWNWLKVKVVVKGGK
                     VVVDKR"
     gene            6296..6961
                     /locus_tag="VN788_12720"
     CDS             6296..6961
                     /locus_tag="VN788_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21437.1"
                     /translation="MLLIFSVPCAYAQGDANRAVANGGISVPGWTGKIDARAEQSGQT
                     IKDSKLTSEGGVLHVTTGPAVTYWNPANKATGNYTVSATFTEPKYMNLNSHPHPYGIV
                     IAGNDMGTAQQSYLYCAAYGNGTFIVRGFGPDPFQMNGSGEENAAVHKAAGVGQPVTQ
                     KIALSVKGDRIECAINGTVVASYTKSELVTSGKLKSTDGVYGIRFAHNTDATVAGLTV
                     SKQ"
BASE COUNT         1500 a         1980 c         2076 g         1528 t
ORIGIN      
        1 tgggtgaccg cgattctggg cctagccgac gacaaggata tagttttgat agacgcccgc
       61 gtggacaaca aaccatccgg atcaaaagcg gcctaggaat tgtctccatc gacggcctac
      121 cagcgcaagg tcctcaggcg gttttggctt ttgctttggc ttgcgcgaat agcgcaagca
      181 tttcgcgatt gaatgcggga atatccgaag gctggcggct ggaaacccaa ttgccatcgc
      241 ggaccacttc ttcatctctc cagtttccgc ccgcgttacg aatgtcgtcc tgaatcgtgt
      301 gatagctggt tagcgttctg cctttcacca gtccagcgga gactagcagc catggcccgt
      361 ggcaaatgac ggcgatcggt ttccttgcgc gatccatttc gcggacaaat tcctgcgctg
      421 ctttttcgac tcgcaatgcg tcggcattca acgcgccgcc gggcaaaagc acggcgtcat
      481 agtcgcccgc gtccgcttcg gccagcggca gatccacatc gacctgtttg gatttatcgt
      541 ggtgttgcat tccttgaatt tcgttgtctt taggagaaat aagtacggtt ttcgcgccgg
      601 ctccttccaa cgcagcccgc ggctgcatca attctgattc ctcgaaacca tccgtcgcca
      661 ggatggctat cttgattcca ggcaattttg tttccggcat aatcgctccc tgatgttggt
      721 cacatcttag tgaaggtgaa gcacggtgtt ttgccagtcc atcggtgact cgctgtaaga
      781 aacaaagcga agcgtggtag gtagggccga atctacctga tggcttcgag gaagctggta
      841 ccgtgcggaa tcgacgcgcc gaaattttcg gccacggttt ggcccatgtc ggcaagcgtc
      901 gagcgagtgc cgagattcgt gccgccactt gcgcgcgggc tgtaggcgag gatggggaca
      961 tactcgcgcg aatggtccgt ggtcggattc acgggatcgg gatcgcagcc gtgatcggcg
     1021 gtgatgagca gcaagtcgga agcgcgcagg gaagaaagga atgggcccag cagtgcgtca
     1081 aattcttcga gcgaactggc gaagccttcc acatctttgc gatggccgta gagcatgtcg
     1141 aaatcgacga ggttcgcaaa aatcaagccg cgcgattgcc gagataaaga ttccgtaagc
     1201 ttcgccatgc cgtcggcatt atttctcgtg gtgtcgtaat ctcgcacgcc gcgcccattg
     1261 taaatgtcgt gaatttttcc cacgccaaag accggcactt tgcgttccgc gagcacatcg
     1321 agaagcatgg gacgcggggg ctcgacggca tagtcatgac gccgctccgt gcgcctgaaa
     1381 tttccaggcg tgcccgtaaa cgggcgagcg atcacgcggc ccacgcgatt cggcccatcg
     1441 agcagcgtgc gcgcgatctc gcacatgcga tatagctcgg caatcggaat cacatcctcg
     1501 tgcgcggcga tttgaaacac gctatcgccc gacgtgtata cgataggaaa tcccgtgcgc
     1561 acgtgctctt cgccgagttc cttgataatt tcggtgccgg aggcgggctt attgccgagc
     1621 gtgcgccggc cgatgcgccc ttcgaatgcg gcgatcactt ctttcggaaa gccatgcgga
     1681 tagactggaa acgcttgctc gagccagatg cccgccattt cccagtgccc ggtggtggtg
     1741 tcctttccgg gagagacggt cgcgcccttg ccgaagctac cctcgggccg cgctggcggc
     1801 gcaagatgct cgagcggttt gatgcttgcg aggccgaggc gaacgagatt cggaatctgc
     1861 aaaggccgcg agcgcgcaat gtggccgagc gtgtcgcgtc ccacgtcgcc gtagtcggca
     1921 gcatccggca actcgccgat tccgacgcta tcgagcacga tccaaatgac gcgctcaaat
     1981 cgagatttgt tttccgccaa agagattcct caattgttcg gtcatcctgc ccgatgtctc
     2041 cgtttcatgc cttgattgac cttgattgac cttgattgac tttttcagtt cgtggttatt
     2101 tcgcggggac tggattcgcg ccgcctggcg ccggttcggc actttcgagc cacgccgtgt
     2161 accagaggtc gagcagcatt tgcgagccgg cggcgagtct ttgctccgta aatttcacgg
     2221 cttccggcgt tccgcttcca ttaaggcttc cggatttatc aaattcgtag atttgcttta
     2281 cgaacccatg cgatacccac aggtatttta gataatcgtt aaacacatcg tccaactggt
     2341 gcggcgctcc cgccattgcg gcaaaatcct ttgcggtaat cgtccggttg acgaaatcgt
     2401 tttcaaaccg ccagtgaatg ccgcgctgcg tggtgaatcc gtccggattg gcgccgaccc
     2461 atccatcgta atcaatcgtc gtatggagag gctgcgatcc atcggcgacg taatgtccca
     2521 gccagcccgc gtagaaaatt gcatcttgct ccacggctcc ggtgtctttt ccagcttgct
     2581 tgagacttcg ataatcgcgg aacgccacct tcagccgctc gaatacttcc atggtgatat
     2641 agggctgcag gcccacattc tcgggtagat agtcgtccgg atgcacctgc gtggccgccc
     2701 gttttgcgta cagcattttg taaaactcgt agcggccctt gggaagcgcc cccaagccct
     2761 cgacccgttc caggtcgatg aaatggtcgg ggctttggga gttgttcaga gcgtattcgg
     2821 aagcgccccg ccagcgatca ggctccggcc cgagatacgc gattcgcgcc actgcggcac
     2881 gggttctgaa aaaaagcggc atggacttag gaatcttgct cgccgcgacg aagtttatgt
     2941 ccgtgtgtcc ccggttgccc catccgaaag cgggaacctc gccgatgagc agcatcataa
     3001 tggccacaac gatccaagat ggagaagcga acgccctcga tagggttttg cctgtcgcat
     3061 tcacgcgtgt tcttcctgtt gtcatgcgga cgtatgcagc tgaaatgtac cggatcgcgt
     3121 cgagtttagc ccgaaattca aaacataggg atgcgaatgt ccggtcctta cggttttacg
     3181 atcattttat tatcgtagtt tgcgggcggc gacttgcaag ctgcagagga cctcgcaccc
     3241 tcccaagcct gcgccggatt ccttcaatcg agcgagcggg cgcggcagca atgggaactc
     3301 gaacgccgct tccatctgtt cgaagcgtcc cgttagtaag gggaccgcct tcaacacccg
     3361 cgtccctcga tgcgtgatat tcccctttga ttaattggca agcggcttgc gagaagttaa
     3421 gccaacaccc gtcgcggtgt gtgcggcggc gtaggaagcg taggggtgat atgcaagata
     3481 tgacaggagt tgcgcccaac gaggtccagg aaaccggtat ccggagagga ctgcttgagg
     3541 acattgcgct gaagacgttg tatctcaatg gtgaaatgtc tttgaccgag ctgagcaaac
     3601 atatgtgcct cggcgggggc gtaatcgacg aaatcttcca attcttccgc aaggaacagc
     3661 tctgcgaagt gaaagggatg gtgggcggaa gccatcgcat agtggcgagc gcccagggca
     3721 ggtcgcgagc ggcaggcctt ctggcgatga gccaatatac cgggccggcg cccgtatccc
     3781 tggccgatta ccgcctccgg gtcaatgcgc agaccatcca acagaccggg atccagccct
     3841 cggatttgaa acgcgcccta cacccgctga ttctcgatgt gggttttgtt gcccggctgg
     3901 gcaccgccgt ggtttcggga acgtcaattt tcctatatgg cccgtctgga acgggaaaaa
     3961 ccagcattgc agcctgcatt ccggccatct atagcgacaa cgtgtggatc ccgtacgcgg
     4021 ttgaggtgga tggacaaatc atctccgttt acgaccccgg cgtacatcgc gagagcggcg
     4081 aaccggtttc cgaggaagcg gataagcgct gggttctttg ccagagaccg cggattttgg
     4141 cgggaggcga gttatcttca gaaatgttgg atattcagct cagctcgaac cgtttctata
     4201 cggctccgct gcagatgaag gccaacaatg gagtgctgat cctggatgac ttcggccggc
     4261 agcgaatgcg ggccgacgaa ctcctcaacc gctggatgac tcccctggat cgccgcgtgg
     4321 attttctcac gttgcccggt ggcaggaagt tcgagattcc ctttgacgtt tgcgtggtgt
     4381 tctccaccaa tcttgacccg cgcgaacttg gcgacgaggc tttcgttcgg agaattccga
     4441 acaagatcca tgtgccacat gccacgcggg cacagttcat ggagatcttt cgcgcggagt
     4501 gcaccatgcg cctgctcgag tgcgaacccg gcttgccgga gcacgcggtt gaagtgatta
     4561 cacaagagct taagcagccc ctcagccagt gttacccgcg tgatctgatt aatcagattt
     4621 tctggagcgc gagttacctc ggtgtggaac cgcggctcac ccgagatgtc ctgaatcaag
     4681 cctgccacga ctatttcgtc tcaacagcca agagctaacc cggcacgctt gtaaacgtgc
     4741 taagtcccgc cgagcctgtt gtgtctctgc ccgcttcccc gaactgaaaa agcgacggca
     4801 gcactagcgt gaggcccggc aaggcagata ggattgcgtt ccgctgcagt agcttgtaca
     4861 ttgccgggtc gcagagaaag cacggaagat tctgcttcac aacatccgat ccctgggaga
     4921 aagcaatgaa tcggttcaat ttgaggctat tcggttgcat tttggtctgc ctgaccttgg
     4981 ggacgttttc gtccctcttc gcgcaggatc tcaccatcag gaacgcgcgc atcatcgtcg
     5041 gcaatggcac agtgattgac aatggatcca ttgtgattcg cggaggaaag atcgtttccg
     5101 cggcggcggg caggcccgcg gccacggccg ggaaagtgat tgacgccaag ggcatgagcg
     5161 ccatgcccgg ttacatagac gcgcacaagc acatcaacaa tttcaaagcc gaccagatgc
     5221 aggatctgct cgaagccggc tacaccaccg ttctttccgg cggcggcagc gcggagagcg
     5281 tcgcgaagct gcgagaccag atcgaatccg gcgccatgaa cgggcctcgg ctgatcgtct
     5341 cagggccggt gaacgtaaga atgggcgccg atatcgcgcg ccagcaggtg gatgatcttg
     5401 ccgcgaaggg cgtcaagtgg acgggtgaga tcgcccttac tcccgaaccg ggcccgagcg
     5461 ccatggaaat cgaagggctt aaggcggccg tggacgaggg cgcaaagaag ggcgttacga
     5521 ttcaggttca cgccgtaagc gtgccggcca tgatggccgc cattgatgcg ggggtgcggc
     5581 acttggtgca cgtcccgaac aagggctggg taaccgtggc ggacgcgcaa aaactgacgt
     5641 ccacagggac gcacatatta gcgaccatcg gcttcggcgc gcctgttttt ggcgtgtttg
     5701 cagacgataa taacccgcgc tttaggaacg gcaaaccctg gccggcgtcc atcgctggcg
     5761 cggaccttct cgatggtgtc gccgtgggcc aggaaatcgg atacacgatg gtgaactcga
     5821 ggacgatttg ggacgcgggc ggcattcttg gttactgcac ggatgacggc tacgaagcgc
     5881 gggctggcct cgagcacgag ctgaaaacct acaacgtgat gttctcgatg aaagacctcg
     5941 tcaaactgat gggtcctaac accgcggact acctcggtat gagcgatcaa ctcggcacgc
     6001 tcgaacccgg caagctggcc gacatcgttc tgcaggacgg taatcccatg gaaggttact
     6061 ggaattggct gaaggtgaaa gtcgtcgtaa agggcggcaa ggtagtggtg gacaagcggt
     6121 aactcaaaaa agagagtact cggaaagata tttcgggccg gaggcgtcac cgggggcaac
     6181 gggagttgtg gctggcctgc aaactcctta taatttgcaa tcgtccgcgc ttttttgttc
     6241 catgaaagga catcacatgc atctctgtcc cattaggacc ttggcggtcg ctgttgtgct
     6301 actcatcttt tcagttccgt gcgcctacgc tcagggggac gcgaaccgcg cagtcgccaa
     6361 tggcggcatc tctgtccctg gctggactgg aaaaatagac gccagagccg agcaatctgg
     6421 ccagactatc aaggactcga agctaacatc agagggcggg gtgcttcacg tcaccaccgg
     6481 tcccgccgtc acctattgga atccggctaa taaggcgacc ggcaactaca cagtgagtgc
     6541 gaccttcact gagcccaaat acatgaacct caatagccat ccgcatccgt acggaatcgt
     6601 cattgccggc aacgatatgg ggacggccca gcagagctac ctttactgcg ctgcgtacgg
     6661 caacggtacg ttcatcgtgc gcggattcgg tcctgatccg tttcagatga acggtagtgg
     6721 cgaggagaat gcagcggtgc ataaggccgc gggagtggga cagccagtca cgcagaagat
     6781 tgcgctctcg gttaaaggtg atcgaatcga atgtgccatc aacggcaccg tcgttgcgag
     6841 ctacacgaaa tctgaattgg ttacatccgg aaaactgaag tccaccgacg gcgtctatgg
     6901 tatccggttt gcccacaaca ccgatgccac ggtagcgggg ctcaccgtta gcaagcaata
     6961 gtcagattag gccgtcgtcc acaatcctac agccacacgc tggttttcga tatcttgcgt
     7021 ccccattatc caatcgggaa tttgatccta gggggaccat tcaaagtcta aagttgcgtt
     7081 gcac
//
LOCUS       DATNDT010000391         5253 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4140460, whole genome shotgun sequence.
ACCESSION   DATNDT010000391 DATNDT010000000
VERSION     DATNDT010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5253)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5253)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5253
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4140460"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="VN788_12725"
     CDS             complement(<1..662)
                     /locus_tag="VN788_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-histidine N(alpha)-methyltransferase"
                     /protein_id="HXU21438.1"
                     /translation="MGSVPRRAAAIAPSHALSEFCSDVVMGLTQPGQKELPSKYLYDS
                     VGSALFDVICMLPEYGLARAGMRMLERYSGEIVGRVPGPAIVAELGSGSGQQTRWLLE
                     ALAGRQRVNYYPIDISGSALSRCQQELGQMDRVSIVGFERAYLDGLQEVAARRRGDEK
                     LLVLFLGSTIGNFERFAGEQFLREVRSILRESDALLLTTDLEKPVATLVTAYDDPTGV
                     TAA"
     gene            complement(665..2014)
                     /locus_tag="VN788_12730"
     CDS             complement(665..2014)
                     /locus_tag="VN788_12730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011492636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HXU21439.1"
                     /translation="MATSGVALKLYPPLFLRLEEARSRTDRLFDLLPPNSFYDRPVAE
                     RNRLIFYMGHLEAFDWNLLAPALDLESHDPAFDNLFSFGIDPVGGGLPTDEPGDWPNI
                     AQVEKYNWRVRDRLDENLRSSRAQLSADPYLKDGTLLQTAIEHRLMHAETLAYLLHNL
                     PAERKTPQAAAFADPRPAPRRRQATIPAGIATLGQSRSAGGFGWDNEFEEQQIEVPSF
                     SVDVFPVTNEEYLKFVRAGGYEDPSYWEPEDWDWVRRGRVAHPRYWVQRSSSRAADPD
                     TQWEYRAMFGTIPLPLSWPVYVSHAEASAFARWAGKKLPTEAQWHRAAYGAPDETELA
                     YPWGSAPPESSRGNFHHQNWDATPVDAHPAGASAFGVEDLIGNGWEWTSTPFAALPGF
                     KRSSFYPGYSANFFDGKHFVMKGGSSRTDACMLRRSFRNWFQPHYPHVYATFRCVEE"
     gene            complement(2121..2726)
                     /gene="rdgB"
                     /locus_tag="VN788_12735"
     CDS             complement(2121..2726)
                     /gene="rdgB"
                     /locus_tag="VN788_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HXU21440.1"
                     /translation="MSAPAFPRLFLASSNSGKLREYRQLAAGSGIEIELVPKFQNLPP
                     FDESAPTFAENAAGKALHYSRLADGLVLADDSGLVVPALGGAPGVHSARYAGPDATDA
                     DRIGKLLRAMEGKEEKDRCARFVCVVAVAMRGRALAVVSDFLDGMIGKESRGSNGFGY
                     DPVFQMLDTNRTYAELPTQEKNRLSHRGRSFSKLVGAFLCR"
     gene            complement(2730..3467)
                     /gene="rph"
                     /locus_tag="VN788_12740"
     CDS             complement(2730..3467)
                     /gene="rph"
                     /locus_tag="VN788_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015739908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HXU21441.1"
                     /translation="MRADGRGPEDLRPMKMTPDFIPSAEGSVLIEMGRTRVIVTATVD
                     DGVPSFLKGTGKGWVTGEYGMLPRSTEERTPRESTRGRQSGRTLEIQRMIGRTLRAAT
                     DLKALGERTVWLDCDVIEADGGTRTASVTAAFVALALALERMTAAGMLRTVPLFDSVA
                     ATSVGIVNEQLLLDLVYEEDSRAQVDMNVVMTGSGQFVEVQASAEGRPYAADELQKLL
                     ELASGGIRKLIKAQQELLHMNFRGRSR"
     gene            complement(3544..4233)
                     /locus_tag="VN788_12745"
     CDS             complement(3544..4233)
                     /locus_tag="VN788_12745"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HXU21442.1"
                     /translation="MGRNVKIGLLCLVIGVIVGLISFRGLHRRVKRLAETQSADEKAR
                     HEVLAPPISTPTDVTAKAKIFWASGPDSVAPVDVELPLSADPVERSKQLLRKLISDPP
                     SSDQRTLPADATLLDFYVLPDGTAVADFSDAVASEMPSGILSEQLAVDSIVRTLEINV
                     PGLRRLKILIHGQEVDTLAGNVDLTGFFDLNSSPQQAVSSAPRSAGSPLQSPAIPSKP
                     PATAALTKTPH"
     gene            complement(4233..5117)
                     /locus_tag="VN788_12750"
     CDS             complement(4233..5117)
                     /locus_tag="VN788_12750"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXU21443.1"
                     /translation="MRYSNGRLLAAATRNTSAMLFAVRHILLLTCIAVATAATLAIPA
                     ASRSASQQQAPLQPPPAPQTIPATPAPAPQTPTGPVIVLDPAHGGTDTGARGPDGVVE
                     KDLVLQIARTMRAELEQRGYRVVTTRNDDSNPSYDDRSGIANAYPGAIFVSIHISSTG
                     KPGTVRVYYDQFGTPIEPPPSGAAVTGVAERAVNPSSGGLIDWNEAQRSFVEASHRLA
                     SLIQVQLTQLFPGSPIVSKAAAIRALRSIDEPAVAIEISSVSASSPGSLAAAAAPLAK
                     AIAASLVAFRHGGAIGAR"
BASE COUNT         1133 a         1613 c         1487 g         1020 t
ORIGIN      
        1 gccgccgtca cgccggtggg atcgtcgtag gccgtcacta aagtggcaac cggcttctca
       61 agatcggtgg taagcaaaag tgcatcgctt tcgcgcaaaa tcgaacgcac ttcgcgcaga
      121 aattgctcgc ctgcgaaacg ctcaaagttt ccaatcgtgc ttccgagaaa aaggaccaac
      181 agcttttcat ctccgcgccg ccgagccgcg acttcctgaa ggccatccag ataggcgcgt
      241 tcaaacccca caatacttac acgatccatc tgacccaatt cctgctggca gcgggagaga
      301 gcgctgccgg aaatatcgat cgggtaataa ttcacgcgct gccggccggc cagagcctcg
      361 agcaaccagc gcgtctgttg gccgcttccg cttcccaatt ccgcaacgat ggcggggccg
      421 ggcacgcgcc cgacaatttc acccgagtat cgctcgagca tgcgcattcc cgcgcgcgcc
      481 agcccatact ccggcagcat gcagatcaca tcgaacagcg cggaacccac cgagtcgtaa
      541 agatacttcg acggaagctc tttctgtccc ggttgggtga gacccatcac cacatccgaa
      601 cagaattcgg acagcgcatg actcggagca attgcggcgg cccgtcgcgg aaccgatccc
      661 attactattc ctctacgcag cggaaggtgg catacacatg cgggtagtgc ggctggaacc
      721 agttccggaa agaccgtcgc agcatgcatg catcggtccg cgacgatcca cccttcatga
      781 caaaatgttt gccgtcgaaa aaattggctg agtagccggg ataaaaggag gagcgcttaa
      841 atccgggcag ggcggcgaat ggcgtcgagg tccattccca cccgttaccg atcaggtctt
      901 ccactccgaa tgcgctggcg cctgccggat gcgcgtccac aggagtggca tcccaattct
      961 gatgatggaa attgccgcgc gacgactcgg gcggcgcgct gccccagggg tacgcgagct
     1021 cggtttcatc cggcgcgcca tatgcggcgc ggtgccactg agcttccgtc ggcagcttct
     1081 ttcccgccca ccgcgcgaaa gcggacgctt ccgcatgact cacatacaca ggccaggaaa
     1141 gcggcaacgg aatcgtccca aacatcgctc tgtattccca ctgcgtgtcc ggatcggccg
     1201 cccgcgagct cgatcgctga acccagtaac gggggtgcgc aacgcggccg cgacgcaccc
     1261 agtcccagtc ttccggttcc caataactgg gatcctcgta gccgcccgcg cgtacgaact
     1321 tcaaatattc ctcgttcgtc acaggaaata catccacgga aaaactgggc acctcaattt
     1381 gttgctcttc gaattcgttg tcccaaccga aacctccggc actacgggac tgccccagcg
     1441 ttgcgattcc cgccggaatg gtcgcctggc ggcgtctcgg cgcgggcctc gggtccgcga
     1501 aggcggcagc ctgcggagtt tttcgttccg ctggcagatt gtgcaacagg tatgcgagag
     1561 tctcggcgtg catcaatcgg tgctcgatcg ctgtctgaag aagcgttccg tctttgagat
     1621 aaggatcggc ggaaagctgc gcacgtgaac tgcggagatt ttcatccagc ctgtcgcgaa
     1681 cacgccaatt gtatttttcc acttgagcga tgttcggcca gtctcccggc tcatccgtcg
     1741 gcaatccccc tcccacggga tcgatcccga aggaaaaaag attatcgaaa gcgggatcgt
     1801 gggactcaag atccagcgcg ggcgcgagca ggttccaatc gaaagcttcg agatgaccca
     1861 tatagaagat cagccggttg cgctctgcaa cgggacggtc ataaaaggaa ttcggaggca
     1921 gcaaatcaaa aaggcggtct gtgcgcgaac gcgcctcttc cagacgcagg aataaagggg
     1981 gatagagctt tagcgccaca ccggaagtcg ccataagtta cctctctaga ggggaatctt
     2041 ttttactccg gcacgtagcg ccagggcaag caatttattc caacgagtta gatgcgaaaa
     2101 gaactgctaa agtcgcgcaa tcatctgcat agaaaagccc caacaagttt gctgaacgac
     2161 ctgccacggt ggctcaaacg gttcttttct tgcgtgggta actccgcata agttctattg
     2221 gtatcaagca tctggaatac cggatcgtac ccaaagccat tggatccgcg cgactccttg
     2281 ccgatcattc catccaggaa gtcggaaacg acggcgagag cgcgcccacg catcgcaacc
     2341 gccaccacgc agacgaagcg ggcgcaccga tccttttcct ctttcccttc cattgcgcgc
     2401 agcagtttgc cgatccgatc cgcgtccgtg gcgtccggac cggcgtaacg cgctgagtgg
     2461 actcccggcg cgccgccgag tgcgggtacc accaagccgg aatcgtccgc gagcacaagg
     2521 ccatccgcaa gccggctgta atgcaacgcc ttccctgctg cgttttcagc aaaagtaggc
     2581 gccgattcgt caaatggagg aaggttctgg aacttcggaa ccagttcgat ctcgatcccg
     2641 gagcctgcag cgagttggcg gtattcgcgc agcttcccgg aatttgacga agctagaaaa
     2701 aggcgaggga acgcaggcgc actcacggtt tatcgcgaac gcccacggaa attcatgtgc
     2761 agcagttcct gttgcgcttt gataagtttc cggatgccac cgctcgccag ttccagcaat
     2821 ttctgcagct catcagccgc ataaggacgc ccttccgcgc ttgcctgcac ttccacaaat
     2881 tggccgcttc cggtcatcac cacgttcata tctacctgcg cgcgagaatc ctcttcgtaa
     2941 accagatcga gcaagagctg ttcgttcaca atccctacgc ttgtggcggc caccgaatca
     3001 aaaagcggca ccgtgcgcag cattccggcg gccgtcatgc gctcgagcgc cagagcgagc
     3061 gccacaaatg cggctgtaac ggacgcagtg cgcgtgccgc catcggcctc gatcacatcg
     3121 caatcgagcc aaaccgttcg ctcgcccagc gccttgaggt ccgtggctgc gcgcagtgtc
     3181 cgcccaatca tccgttggat ttccagcgtc cgcccggact ggcgtccgcg agtcgattcc
     3241 cggggcgtcc gctcctcggt cgaacgcggc agcattccgt attcgccggt cacccatcct
     3301 tttcccgttc ccttcaaaaa ggacggcacg ccatcgtcca ccgtggccgt cacgatcacg
     3361 cgggtgcgac ccatttcaat cagcacgctg ccttcggcgc tcggaatgaa atccggcgtc
     3421 attttcatcg gccgcaaatc ttccgggccc cgcccatctg ctctcaaaga aaaactcctt
     3481 ctctcgcggt ttgtatcgcg attcagtttg actacggtca acgaatgtgt caatcgcttg
     3541 aaattagtgc ggcgtttttg tgagcgcggc ggtggcgggc ggctttgacg ggattgcagg
     3601 gctttggagc ggcgagcctg cgctccgcgg cgcagacgaa accgcctgct gcggagacga
     3661 attcaaatcg aaaaagccag tcagatcgac attccccgca agcgtatcta cttcttgtcc
     3721 atgaatcaga attttcagcc gccgcaaccc gggcacgttg atttccagag tccgcacaat
     3781 ggagtccacc gccaactgct cgctcaaaat ccccgagggc atttcagaag cgacggcatc
     3841 ggaaaaatcg gccaccgcgg tgccatcggg aaggacgtaa aaatccagca gtgttgcatc
     3901 cgcgggaagt gtgcgctgat ccgagctcgg aggatccgag atgagcttgc gcaacaactg
     3961 cttagaacgc tccaccggat cggcggaaag cggcaattca acatccaccg gggccacgct
     4021 atcgggacca gacgcccaaa aaattttcgc ctttgcggtg acgtccgtgg gagtggagat
     4081 gggcggagcc agcacttcgt ggcgcgcttt ttcgtccgcc gactgcgttt ccgccaagcg
     4141 cttcactcgg cggtgcaagc cacgaaagct gatcagcccg acgatcacgc ctatcaccag
     4201 gcacagcagg ccaattttta cattccgccc cattatctgg ctccgatcgc gccaccgtga
     4261 cgaaaagcga ccaagcttgc ggcaatcgct ttcgcaagag gcgctgcggc cgccgccaat
     4321 gatccgggac tcgatgcgga cacgctcgag atttcgatcg caacggctgg ttcgtcgatc
     4381 gatcgcaagg cgcgaatcgc ggctgccttc gaaacgatcg gcgaacccgg aaaaagctgg
     4441 gtcaattgca cttgaatcag gctcgcgagg cggtggctgg cctcgacgaa tgaccgttgc
     4501 gcttcgttcc agtcaatcaa gccgcccgac gagggattca ccgctcgctc agctacgcca
     4561 gtcacggcgg cgccgctggg cgggggctct ataggcgttc cgaattgatc gtagtaaacc
     4621 cggacggttc cgggcttgcc cgtggaagaa atatggatgc tgacgaatat cgcacccggg
     4681 taggcattcg caattccgga ccgatcgtcg taggaaggat tcgagtcatc attccgggtg
     4741 gttactacgc gatagccgcg ctgctccagt tccgcacgca tcgttcttgc aatctgcagc
     4801 accaagtcct tttcgaccac gccatcgggt ccgcgcgctc cggtatccgt gcctccgtga
     4861 gcgggatcga gcacgatgac cggacctgtc ggagtttggg gagccggagc gggggtcgca
     4921 ggaatggtct gcggagcggg cggtggttgc aaaggagctt gctgttgtga agcactccga
     4981 gaggccgcgg gaattgcgag tgtcgcggcg gtcgcgaccg cgatacaagt cagcaacagg
     5041 atgtgtcgca cggcaaaaag catagcagaa gtattgcggg ttgccgcagc gagaagtcgt
     5101 ccgttgctgt aacgcattat agtttcggaa ccggagaaac atcgaaaatg tgggcttgaa
     5161 atgcctcgcg gcgtacaaaa agtcccgcgc tccgcacgtc gtcacccgaa cgaacgtagc
     5221 gaatatcatt cagctcgtct tggaagacgt att
//
LOCUS       DATNDT010000392        12810 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3246261, whole genome shotgun sequence.
ACCESSION   DATNDT010000392 DATNDT010000000
VERSION     DATNDT010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12810
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3246261"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..789
                     /locus_tag="VN788_12755"
     CDS             <1..789
                     /locus_tag="VN788_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21444.1"
                     /translation="AGLPMNTGKWRTDPDGMAEMYQSQEMMAVIFDVDGETRSGVKMD
                     NGETAAWMKKYPETFIGFGSVDPWKGKIAVNEVKRCADMGLRGMKFQQAAQGFNPNDE
                     RFCPMYEEIVKLNLPVIFHTGTTAVAAGSPGGNGITLEYCKPIPYIDNLAAWYPEMRI
                     IMAHPSWPWHDDQLAVLRHKGNTYMDLSGWAPKYFPASTVHNINTLVQDKAFFGTDFP
                     MMTPERWLSEFAELPLKDSVRPKILLLNAAKFLGIEVPAAQPVS"
     gene            complement(968..1897)
                     /gene="prmC"
                     /locus_tag="VN788_12760"
     CDS             complement(968..1897)
                     /gene="prmC"
                     /locus_tag="VN788_12760"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HXU21445.1"
                     /translation="MPIDPHCRPQSIAMDVREALKEGMAQLRAAAVPSSTLAAELLLM
                     HALDRDRTWLYTHPEDGLDASVARKYFAMIARRAAGEPTQYLTGKQEFWGLEFEVNPD
                     VLIPRPETEHVIEVALERLGARGIKINMRTGEPNPPLRIADVGTGSGCLAVALAHELP
                     HAEIFATDISTAALEVARRNSARHGVADRIRFLQGDLLEPAPHASRSFHLIVSNPPYV
                     AQDEAATLAREVREHEPHTALFGGPSGIEIYERLIQQAGELLRSSGVLVLELGYNSAQ
                     HVRAILVDQRNWVNVSVTDDLAGIPRVLGAERA"
     gene            complement(1897..2298)
                     /locus_tag="VN788_12765"
     CDS             complement(1897..2298)
                     /locus_tag="VN788_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002624291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4180 domain-containing protein"
                     /protein_id="HXU21446.1"
                     /translation="MPARTYELHGVLIFECATESPALRSDRDAVDLMSEAAGHGARFI
                     VIPAERLDDDFFRLETRIAGEVIQRFAMYRVRVAILGDISRHVAQSKSLRDFVHECNR
                     GNQCWFVDREELGRRLERQKAQMEDLGGEAK"
     gene            complement(2361..3464)
                     /gene="prfA"
                     /locus_tag="VN788_12770"
     CDS             complement(2361..3464)
                     /gene="prfA"
                     /locus_tag="VN788_12770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HXU21447.1"
                     /translation="MAKLTLFEKLEQIESRYNEMTRELSSPEVHDDSARYQKLAKTHS
                     ELGEIVAKYREWKEIERGIEGAKQLFAESDDAEMKQMAHDEQRELDARRETVEREVKL
                     LLVPKDPNDEKNVILEIRAGTGGDEASLFAAELFRMYSRYAESQRWRVEILESSASPV
                     GGMKEVVAAIQGNKVYSRLKYESGVHRVQRVPATEQQGRIHTSAATVAVLPEADDIDI
                     KIEPKDLRIDTFCSSGPGGQSVNTTYSAVRITHIPSGLVVSQQDEKSQIKNRAKAMRV
                     LRTRLYEMEQEKQHQALAAERRGQIKTGDRSEKIRTYNFPQNRITDHRINFTVHQLTE
                     AVDGKLGPLVDALVTHFESERLKQELDADADAA"
     gene            complement(3634..4461)
                     /gene="trmD"
                     /locus_tag="VN788_12775"
     CDS             complement(3634..4461)
                     /gene="trmD"
                     /locus_tag="VN788_12775"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HXU21448.1"
                     /translation="MRFDIVTIFPDYFRGPLDYGIVRRAREAKLIETHVHDLRDFTHD
                     RHRTVDDRPFGGGEGMVMKPEPIFEAVGSLIATECKAGQNEDGGVVPRGTAIVLLSAA
                     GRLFRQEAARVLAKLDRIVMVCGRYEGVDERVAEHLATDEISIGDFVLSGGELPAAMI
                     LDAVTRLIPGALGNEDSSVNESFSESTREIDLVQDDRESRARRKNSGICGGSALRIAE
                     RGILDFPHYTRPQSFRGWTVPEVLISGNHEEIRRWRAEKALEKTLRNRPDLLEQFRG"
     gene            4917..5783
                     /locus_tag="VN788_12780"
     CDS             4917..5783
                     /locus_tag="VN788_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HXU21449.1"
                     /translation="MADQPNKTVPPKAAAPRPPEKLPIPGVKHLIAVASGKGGVGKTT
                     VAVNLAVALRNLGAAVGLLDADVYGPNVPIMLGSSEQPKMLDEKTIIPVQAYGVKMIS
                     MGLLNPGDKPLIWRGPMLHSVMQQFLRSVRWGQLDYLIADLPPGTGDVQLSLIQSVAV
                     SGAIIVTTPSVVALADVRKAVEMFRQVNVEILGVVENMSYFSCPHCQGKIDVFGHGEG
                     ENMAKVFEVPFLGEIEIDPQIRIGGDTGKPAASLGEDAPGAKNIYVIARNVVTRTAAA
                     AEAASTPTVQIL"
     gene            5979..6692
                     /locus_tag="VN788_12785"
     CDS             5979..6692
                     /locus_tag="VN788_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019465960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HXU21450.1"
                     /translation="MRPPRNPGRVAGIWYLLLTLIGPLRLMYIPTKLFVHGNATATIN
                     NIAAHERLFRFGILGDLASAIILIFLVLAFYRLFKAVDQNLAVLVVIFGGVMPAVIYF
                     IGVVSDFAVITLVRGQDFLSVFDKPQRDALAMLFLRLRDHQNTAAEVLWGIWLLPLAA
                     LVYKSRFLPRFLGVWLAINGFAYMIISVTGLLLPQYQVKVFNMSFPALLGEVAIMLWL
                     VIRGAKPPAPNAALSSANV"
     gene            6853..8190
                     /locus_tag="VN788_12790"
     CDS             6853..8190
                     /locus_tag="VN788_12790"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HXU21451.1"
                     /translation="MAKSRSSGARRAKSIAPKAARQADKKLKIALIGFGTVGRSVAKI
                     LCKDKNSPLLLTHIFNRNIERKRIAGLPARVRWTASVEDIFSSDADVVVELIGGLEPA
                     GNWVRRALRAGKSVVTANKLLISESGSELLDLARKMGRRLEFGASVAGGIPAIIGIQE
                     GLAGDRLHKIAGILNGTCNFILTKMESTGATFVGALKEAQSLGYAEADSSADVDGSDA
                     RAKLVILIQAGLRLRVRSEQIPCRSIASIEAADFMYASELKSAIRQISIAQKDTSKGL
                     RLAAAVQPALIPAASLAAHAQGSQNLVTVTGEFGGRTIFSGNGAGGDPTAVAVISDLH
                     SIARSASTAPESLPGSLEIPDVVSGDFTVPHYLRFVVRDNPGIVAALAAILAKYQISI
                     DALLQKPGFPHSALSFVMTLEACDSAVLNRALAEIARLDFHVEPPLCLPIFLD"
     gene            8198..9031
                     /locus_tag="VN788_12795"
     CDS             8198..9031
                     /locus_tag="VN788_12795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017715557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXU21452.1"
                     /translation="MAQPQIDANAFHRFEHEGWQQASDDYHRYFGSLTSQTIGPLLDA
                     VATGAKTNLLDIATGPGYVSAEAQRRGWVPVGVDFSGAMVAMARKLHPTIDFQIGDAE
                     ALAFGDAQFGTAVMNFGILHLAQPESAIREAYRILRPGGFFGFTVWAKPEEAVGFAVA
                     LDAIREFGDPNVQLPAGPPFFRFSEPAESTSVLKNAGFENPTVAQIPLVWILASGADV
                     FEAFYRGSARTGGLLRAQPASALAKVRGAIEKRAAVYAATEGRLKIPMPALVVSARKP
                     R"
     gene            complement(9106..10173)
                     /locus_tag="VN788_12800"
     CDS             complement(9106..10173)
                     /locus_tag="VN788_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU21453.1"
                     /translation="MLKRLSVFLGFVALPLFCAVSSLGQESAPPSINVTVDLVQLNVA
                     VTDHKGNYITGLRPEDFVITEDKIPEKIATFGEGDESVRVLLGAAPAGAKPSDPPTQS
                     KNSSGNVSESELTSLVGGANVFVLFDTSNYMYRGFVFAQDAIADFVRSLEKADRIALY
                     SYSRDLSRAAMLTSDRAQVLRGVRTTVAGDDAALYNCLLLTVKDAAHLTGRKVIVVFS
                     NGPDNASMVPPEEVAELAQSTGTTIYMVSTQQAHLEPISNAVFQRMTAATGGKVYFAN
                     NWKDEQNAFASIRDDLAHLYSLSYYPEPNPNRGWRTITVKLRNRQHKGYHIRTRDGYR
                     LQHSRFSTENVQPSQPLPNAQ"
     gene            complement(10255..>12810)
                     /locus_tag="VN788_12805"
     CDS             complement(10255..>12810)
                     /locus_tag="VN788_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21454.1"
                     /translation="VPILLTEQDWTAASEHGGKGENGRDLLDALLHDPVLAHLYYAWA
                     HIDPEIRTTLRQSPGLKRLEPLADSLDFYGSYLRVQSGHVVVPGGSGAESAWKNLVGS
                     SPQSPSEFVPRLLAKDNGWLAAYFDSLSRIPPEEQAHFAEPPRLQRCYEALRGKDALP
                     GAMASVFRPDPDLLVLMTRLQWEPDGEPHVPGNLQAWERIFGQKTSSKATRNLGIRAR
                     SLKDPEGLLEALFAFSRVREEGGPTEIFLALNELDSRRPAGRRLGPQAVELMAGRYAE
                     FKDQYLIFSEFPELSDASIAEFLRTASALERIREHTLRGNAMGIFEANVSLWQILSRQ
                     GEIAANKQDGSWQAMVKPFGHVSSATQLFDAGHTSLGEVLRAATGEPQSSQDEIVDLL
                     AGPRQTSREGQRIHKELAERIHAVLDDQRLVSLDTLLELGDGLTAMAQAAPGSATGDK
                     LVPLAEQLTEFQMPQPIFTRGERTEFTAGTYNNRHTELEMRTDVAKILQAPASRAQLE
                     EARGQLAPFLRDTLVGFSYAYYQPPGAQALHNNSLVIRSHDFSGETIMGLEGSLWQTP
                     RLFGAGSPAGGGAHLVGSLANLPYVLAKMEEDFIAPENVQALIWSDLVPSVLTGAVLP
                     RWWGVSRNELHAVALYQRAGEELIAASVENEDLRGKVMTLLSDRMPPQRLAQLEDTLR
                     AGHPAKMITPADSFYLTAEFRQKFPGDTSSWGPAGRELEKLARDNPAELSWDRLSQDF
                     GVPHPTLARTYSRELLNLRLFPMFAGYSSRLFAETWDSTNLYWARLADEMGYQPETLN
                     RLVPELTVRMVAKIFATDLNDWPAILRAMRETGEEFRQGKLASLGLNGGPPQP"
BASE COUNT         2635 a         3777 c         3578 g         2820 t
ORIGIN      
        1 gcgggtctgc cgatgaacac gggaaaatgg cgaactgatc ccgacggcat ggcggagatg
       61 tatcagagcc aagaaatgat ggccgtgatt ttcgatgtgg acggcgagac gcgcagcggc
      121 gtgaaaatgg acaatggcga aactgccgcg tggatgaaaa agtatcctga gacgttcatc
      181 gggttcggta gcgtcgatcc atggaaggga aagatcgcag tcaatgaagt gaagcgttgc
      241 gcggacatgg gattacgcgg aatgaaattt cagcaggccg cgcagggctt caatcccaat
      301 gacgagcgct tctgccccat gtacgaggag atcgtcaaac tcaacctgcc ggtaattttt
      361 cacaccggca ccactgccgt cgccgcaggc tcccccggcg gaaacggcat caccctcgag
      421 tactgcaagc cgattcctta tatcgacaat ctcgctgctt ggtatccgga aatgcgcatc
      481 atcatggcgc atccgtcatg gccatggcat gacgatcaat tggcggtgtt gcgccacaag
      541 ggcaacacct atatggatct ttcgggctgg gcgcccaaat atttccccgc gtcaacggtg
      601 cacaacataa atactcttgt gcaggataag gcttttttcg gcaccgattt ccccatgatg
      661 accccagaac gctggctgag tgagtttgcc gaacttccgc taaaggattc ggtgcggccc
      721 aaaattttgc tgctcaatgc cgcgaagttt ttgggaatag aagtgcccgc cgcgcagccc
      781 gtgagctaga tttgccactt gtcttcagcg ggtcctgttt atatcgaaga gagattcttc
      841 gactgcgtgt cccggcgctt cgcgcaaaac taaaagcacg ggacactccg ctcagaatga
      901 cagcgtaaaa aaccaaaaag cgcaggataa aatgaatcgg cgtgttgcgc tgctctgttg
      961 aagccgctca ggcgcgttcg gcgccgagca cgcgaggaat tcctgcgaga tcgtcggtca
     1021 cgctgacgtt cacccagttc cgttggtcca ccaaaatcgc gcgaacgtgt tgggccgagt
     1081 tatatcccag ctccagcact agcacgccgc tagatcgaag taattcaccc gcttgttgga
     1141 tcagccgctc gtagatttcg atgcccgaag gcccgccgaa aagggcagtg tgaggttcgt
     1201 gctcgcgaac ctcacgcgcc agcgtcgccg cctcgtcttg cgccacgtaa ggaggattgc
     1261 tgacgattaa gtggaatgat cgagaagcgt gtggagccgg ttccagaaga tcgccctgta
     1321 aaaagcgaat gcggtccgca acgccatgcc gtgcagaatt gcgacgagcc acttcaagcg
     1381 ctgcggtcga aatatcagtg gcaaagatct cggcgtgcgg caattcatgc gccagagcca
     1441 cagccagaca ccccgagcca gtgcccacgt cggcgattcg cagcggcgga tttggttcgc
     1501 cggtacgcat gttgattttg attccgcggg ccccaaggcg ctccagagcc acttcgatta
     1561 cgtgctccgt ttctggccgg ggaatcagca cgtccggatt gacttcgaat tcgagtcccc
     1621 aaaattcctg cttgccggtg agatattgtg ttggctctcc cgccgctcgt ctggcgatca
     1681 tcgcaaagta ttttcgcgcc acggaagcat cgagcccgtc ttccggatgc gtatagagcc
     1741 aagttcggtc gcggtcgagg gcatgcatca gaagcaattc ggcagcaagc gtagaggacg
     1801 ggaccgccgc cgcgcgcagt tgcgccatgc cttctttcag cgcctcacgc acatccatcg
     1861 cgatggattg cggcctacag tgtggatcaa ttggcattac ttagcctcac cgccgaggtc
     1921 ttccatttgg gctttctgcc gctccaaccg cctgccgagt tcttcgcggt caacaaacca
     1981 acattgattg ccgcggttac attcgtgcac aaaatccctt aaggacttgc tctgagccac
     2041 atggcgggaa atatcgccaa gaatcgccac gcgcacccga tacatcgcga accgctggat
     2101 cacctcgccc gcgatgcgcg tttccaaccg aaagaaatcg tcgtcgagcc gctcggcggg
     2161 aatcacaatg aatctcgcgc catggccagc cgcctcgctc atcaagtcca cggcatcgcg
     2221 atcactccgc agggcggggc tctctgtagc gcactcaaag atgaggacgc cgtgcagttc
     2281 gtatgttcgg gcgggcacta ttttggcgac cccctgtggc gacgaaaact ttagaagcgg
     2341 cgaggtatgt tggccgccaa ctatgctgcg tccgcgtctg cgtcgagctc ttgtttcaga
     2401 cgctcggatt cgaaatgagt gaccagcgca tcgactagcg ggccgagctt gccgtccacc
     2461 gcttcggtta gctgatgaac cgtaaaattg atccggtgat cggtgatccg attctgggga
     2521 aaattataag tgcggatttt ttcggaacgg tcgcccgtct tgatctgccc gcggcgctcc
     2581 gcagctagcg cctgatgctg tttttcctgc tccatttcat ataatcgcgt gcgcagtacg
     2641 cgcatcgctt tggcgcgatt ctttatctgc gatttttcgt cctgctgcga aaccaccagc
     2701 ccgctgggaa tatgggtgat acgcaccgcc gaatacgtgg tgttcaccga ttgtccgccc
     2761 gggccggatg agcagaacgt gtcgattcgc aaatctttcg gctcgatctt gatgtcaatg
     2821 tcgtccgctt cgggaagcac cgcgacagtc gcggccgagg tgtgaatgcg gccttgttgc
     2881 tcggttgcgg gcacccgctg cacacgatgc acgccgcttt cgtatttcaa ccgcgaatac
     2941 actttgttgc cctgaatggc ggccaccact tctttcatgc cgccaaccgg ggatgcggag
     3001 ctttcgagta tctccacgcg ccagcgctgc gattccgcgt aacgcgaata catgcgaaag
     3061 agctcggcgg caaaaagcga ggcttcgtct ccaccggtgc cggcgcgaat ttcgagtatc
     3121 acgttttttt cgtcgttggg atccttgggc acgagcagca attttacttc ccgttccacg
     3181 gtctcacggc gggcgtcaag ctcgcgctgt tcgtcgtgcg ccatctgttt catttcggcg
     3241 tcgtccgatt cggcgaacaa ctgcttggcg ccctcgatgc ctctttcgat ttccttccac
     3301 tcgcgatatt tggcaacgat ttcgcccagt tccgaatgtg ttttggcgag cttctggtag
     3361 cgcgccgaat catcgtgcac ttccggcgag gagagctcgc gcgtcatctc gttgtagcgc
     3421 gattcaatct gctcgagttt ttcgaaaagc gttaatttcg ccatggaaca gctcgcactc
     3481 gaacctgcaa ccgccattta tagatgcaaa aattttcaaa gggatgcgtg agccgctgtg
     3541 cgccagcccg agaagggaat tgctaatgga atagagagtt catatccaca accactaact
     3601 caagcataaa gggaagtagc ttgctagtca aattcaaccg cggaattgtt ccagcaggtc
     3661 agggcgatta cggagtgtct tttccagcgc cttttcggca cgccagcgac gaatctcctc
     3721 atggtttccg ctgataagca cttcagggac tgtccacccg cgaaatgatt gaggtcgcgt
     3781 gtaatggggg aaatccagaa tgccgcgttc cgcgatccgc agcgcgctcc cgccgcatat
     3841 gccgctgttc tttcgccggg cccgcgactc acgatcgtcc tgcacaaggt caatctcgcg
     3901 ggtggattcg ctaaacgatt cgttcacgct cgaatcctcg tttccgagtg ctccgggaat
     3961 aagccgtgtc acggcatcca gaatcatcgc ggcggggagt tcgccgcccg acaggacaaa
     4021 atcaccaatc gaaatttcgt cagtggccag atgctcggcg acgcgctcgt caacgccctc
     4081 gtagcgtccg cagaccatta cgatgcgatc gagcttcgcc aatacccggg ccgcttcctg
     4141 ccggaaaagc ctgcccgccg ctgacagcaa cacaatcgct gttccgcgcg gcacaacgcc
     4201 gccgtcttca ttttggccag ccttgcattc ggtggcgatc aacgacccga ccgcttcgaa
     4261 tatcggctca ggcttcatca ccattccctc gccgccgcca aaaggcctgt cgtccactgt
     4321 gcgatgccgg tcgtgcgtga aatcgcgcag atcgtgcacg tgcgtctcga tcaatttggc
     4381 ttcgcgcgcc cgccgcacaa tcccatagtc gagcggaccc cgaaaatagt ccgggaaaat
     4441 cgtgacaatg tcgaatcgca tggctgccta tcctacctta ggaacgggtg ggttggttca
     4501 cccgggaact tggcgacttc cttagtttat ctggcggaca gaatgacggc tcgacatcgc
     4561 ggtatgcgcc attccgggcg gtattgcggc tggcgtaaac tcggccaata gggcgtagta
     4621 cgaggataca aacagaatct ttacaccgca ggcctaaaac gcgcaagtgg ttgttggtgt
     4681 aaagaaaaat cgtccacggt gagtgtggac ttttggggtt tgtgtcgtag ccaaggctgg
     4741 aacggtctaa aatggccatt ttcggatgga tcgcgctgcg gcgttgccgc ctgcccgcga
     4801 gcagtctatg atggctatgc cgccgatgcg gagcgcgaag agtcggaaca agaactcccg
     4861 agttttaccg cggctatagc gaattcaagc aaccacaggg gagaaccgga actaggatgg
     4921 ccgatcaacc caacaaaacg gtgcctccta aggcggccgc gccacggccg cctgagaaac
     4981 tgcccattcc cggcgtcaag cacctgatcg cggtagcgag cggcaaaggc ggcgtgggca
     5041 aaaccaccgt tgcggtaaac cttgccgtcg cgctcaggaa tctaggcgcg gcggtcggct
     5101 tgctcgacgc agacgtgtac ggccctaatg tccccatcat gctcggttcc agcgagcagc
     5161 cgaagatgtt ggatgagaaa accatcattc ccgtgcaagc ctacggcgtg aagatgattt
     5221 ccatgggcct gcttaatccc ggggacaagc cgctcatctg gcgaggcccg atgctccata
     5281 gcgtgatgca gcaattcctt cgcagcgtgc gctggggcca gctcgattac ctgatcgcgg
     5341 atttgccgcc cggcaccggc gacgtgcagt tgagcttgat tcaaagcgtg gccgtcagcg
     5401 gggctattat cgtcaccacg ccatccgtgg tcgcgctagc ggacgtgcgc aaggccgtcg
     5461 aaatgttccg gcaggtgaac gttgaaattc tcggagtggt cgaaaacatg agttacttct
     5521 cttgcccgca ctgccagggc aaaattgatg tcttcggcca tggcgaaggc gagaatatgg
     5581 ccaaggtttt cgaggttcca tttctaggcg aaatcgagat cgatcctcag atccgcatcg
     5641 gtggcgatac gggaaagccg gctgcctctc ttggcgaaga cgcgcccgga gccaaaaaca
     5701 tttacgtcat agcccgcaat gtggtcaccc gaaccgcagc ggcagcggaa gccgcctcaa
     5761 cccccaccgt gcaaatcctc tagctttagc cgaagacgca agctgcccgc cgcaccagga
     5821 tgagatccaa tcaatatgtc tggcgtccgc accgccatcc ggcacgcgga tttcgacgcg
     5881 gaatagcagc ctcaccgttc cgtgatgaaa ctttatgttg gccttgggcg tatcttgcgc
     5941 tagacccttc agtagcccct ctaccgagga agggaattat gaggccgcct cgcaatccag
     6001 gaagagtcgc tggaatctgg tacctgcttc tcacgctaat cggccctcta cgcctcatgt
     6061 atatacccac caaactgttc gtgcatggga acgcgaccgc gacgatcaac aatatagccg
     6121 ctcacgaacg gcttttccgg ttcggcatcc tgggtgactt ggcatctgcg attatcttaa
     6181 tttttctcgt gctggctttt taccggctat ttaaggccgt ggatcagaac ctcgctgtgc
     6241 tggtggtgat cttcggcggc gtgatgcctg ccgtcatcta cttcattggc gtggtgagcg
     6301 actttgccgt tataaccctg gtgcgcgggc aagatttctt atccgtattc gacaaacctc
     6361 aacgggacgc gctcgccatg ctgtttctac gtctgcgtga tcatcaaaac accgcagccg
     6421 aggtgctttg gggaatctgg ctcctcccat tggccgcgct cgtgtacaaa tcgcgtttcc
     6481 tgccgcgctt tctgggcgtc tggctcgcca tcaacggatt tgcctacatg atcataagtg
     6541 tcaccggcct gctgttaccc caataccagg tcaaagtatt caatatgtcg tttccggcgc
     6601 ttttggggga agtggcgatc atgctgtggc tcgtaattcg aggtgcgaag ccgcctgcgc
     6661 caaacgcagc cctatcgtcg gcaaatgtct aacgatggcg gctcccagac gacgttcgca
     6721 atctgcgatt tgtacacgaa tagaagcctc tctcttcatt gtttgatacc atgtgtcgcc
     6781 gggatatctc tgcacctggc cgattcgcgc aacatccagc aggcaaattt cttgatgtga
     6841 ggttttccat cggtggccaa atcgcgttcg tccggcgccc ggcgcgcaaa gtccattgcc
     6901 ccaaaagccg cccggcaggc ggacaaaaaa ctgaaaatcg ctctaatcgg cttcggcacc
     6961 gttggccgtt cggtcgcgaa gatcctatgt aaagacaaaa atagcccgct cctcctcacg
     7021 cacattttca accgcaacat tgaaaggaaa agaatagcgg gactcccggc ccgtgttcgc
     7081 tggaccgcca gcgtggaaga catcttttcc tcagacgctg atgtcgttgt cgaactcatt
     7141 ggaggcctcg aacctgccgg aaactgggtg cgccgcgccc tccgtgccgg aaaatccgtg
     7201 gtcaccgcca acaagctcct gatttcggaa tccggttcgg agttgctcga tctcgctcga
     7261 aaaatgggcc gccgcctcga gttcggtgcg tccgtcgccg gaggcattcc cgccattatc
     7321 ggcatccagg aaggccttgc cggtgaccgt cttcacaaaa tcgcaggaat cctcaatggc
     7381 acctgcaatt tcattctcac gaaaatggag tccacgggcg cgaccttcgt cggcgcgctg
     7441 aaggaagcgc aatcgctcgg ctatgccgag gcagattcca gcgccgatgt agatggaagt
     7501 gacgcgcgcg ccaagctagt tattctcatt caagctggcc tgcgccttcg cgttcgcagc
     7561 gaacagattc cctgccgctc gatcgcctcc atcgaagccg ccgacttcat gtacgccagc
     7621 gaactcaaga gcgcgatccg ccaaatctcg atcgcgcaga aagatacttc gaaaggcctc
     7681 cggctggctg cggccgttca gcccgcgctc attcccgccg cttcgttggc cgctcatgct
     7741 cagggcagcc agaacttggt cactgtgacc ggcgaattcg gcggccggac gattttttca
     7801 ggcaatggag ccggtggaga tcctacggcc gtcgccgtga tttccgattt gcactcgatt
     7861 gcacggagcg cgagcacagc tcccgaatcc ttgcccggct ctctggaaat ccctgacgtg
     7921 gtctccggcg attttaccgt ccctcactat ttgcgcttcg tggtgcgcga taatcccggc
     7981 atcgtggccg ccctcgccgc gatcctcgcg aaatatcaga tcagcataga cgcgttgctg
     8041 caaaaaccgg gcttcccgca ttccgctctg tctttcgtga tgacccttga agcctgcgat
     8101 agcgccgtcc tgaatcgtgc gctggccgaa atcgcgcgcc tcgatttcca cgtcgagccg
     8161 cctctatgtc tcccgatttt tctggactga ggtttcgatg gcgcagccgc aaattgacgc
     8221 aaacgctttc catcgattcg agcatgaagg ctggcagcaa gcttcggacg attatcatcg
     8281 ctacttcggc agtctcacgt cgcagaccat cggcccgctt ctcgacgcag tagcaactgg
     8341 tgccaaaacc aatctcctcg atatcgccac cggaccagga tacgtttccg ccgaggccca
     8401 gcgtcgcgga tgggtgccgg tcggcgttga tttttccgga gctatggtcg cgatggcgcg
     8461 gaagcttcat cccacgattg attttcaaat tggcgacgcc gaggcacttg cgttcggcga
     8521 cgcgcagttt ggaaccgccg tgatgaattt cggcatcctg catctcgcgc aaccggaatc
     8581 tgcaattcgc gaggcttatc gcattctgcg tcctggcggg ttcttcggtt tcacggtgtg
     8641 ggccaagccg gaagaggctg taggattcgc tgtcgccctt gacgccattc gcgaatttgg
     8701 cgatcccaac gtccagcttc ccgccggacc gccgtttttt cgtttcagcg aacctgcgga
     8761 gtccacgagc gtcctcaaaa acgccggctt cgagaacccc accgtcgcgc aaatcccact
     8821 ggtatggata ttagcttccg gggcggacgt attcgaggct ttttatcgag gctcggcgcg
     8881 gacaggcggc ctgctgcgcg cgcaacctgc ctcggcgctt gcgaaagtgc gaggagcgat
     8941 tgaaaagcgc gcagccgtct acgccgccac cgaaggccgc ctcaaaatcc ccatgcccgc
     9001 cctcgtagtc tccgcccgga agccccggta gccgcgagga ctgcctttct cgccgcaggc
     9061 atccgctttt cttcaagctc tagatgcgat accgcgctag agggcctatt gtgcgttagg
     9121 gagtggctga ctgggctgaa cattttctgt ggagaaacga gaatgttgga ggcgataccc
     9181 atcccgcgtt cgaatgtgat accccttatg ctgccggttc ctgagtttta cggtgatggt
     9241 gcgccagccg cggttgggat ttggttcggg gtaatagctc agagaataga gatgagccaa
     9301 gtcgtctcgg atcgaagcaa acgcgttttg ctcatccttc cagttattag caaaataaac
     9361 ttttccgcca gtggcagccg tcatccgctg aaataccgca ttggagatcg gttccaggtg
     9421 cgcctgttgg gtacttacca tgtagatcgt cgttccagtc gattgcgcca actcggcaac
     9481 ttcctcgggt ggcaccatgc tggcgttgtc ggggccgttt gagaaaacga cgatcacctt
     9541 tcttccagta aggtgggctg cgtccttcac tgtcaataac aggcaattgt aaagtgccgc
     9601 gtcgtctccc gccacggtcg tgcgtacacc tcgcaaaacc tgcgcgcggt cggaggtgag
     9661 catcgcggct ctcgaaaggt cgcgactata ggagtagagg gcaattcgat cggccttttc
     9721 gagggatcga acgaaatcgg cgatggcatc ctgcgcgaac acaaaaccgc gatacatgta
     9781 gttgcttgtg tcgaaaagaa cgaaaacgtt cgcgcctcct accaaagaag tcagctcact
     9841 ctcggacacg tttccgctgg aattctttga ttgcgttggc ggatctgacg gtttggcgcc
     9901 ggccggtgcg gcgcccaata gtacgcggac cgactcgtcc ccttctccaa aagtggcgat
     9961 cttctctgga atcttgtcct cggtgatcac gaagtcttcc gggcgcaggc cggttatgta
    10021 gttccccttg tggtcggtga ccgccacgtt caattgcact agatcgacgg tgacgttgat
    10081 ggacggcggg gcgctctctt gacctagcga tgagactgcg cagaatagcg ggagtgccac
    10141 gaagccgaga aacacagaaa gtcttttgag catatagtcc tcaatttcga aggcttagcg
    10201 ctatcgtcca gccggttagg tcgcgtccag tcccgcacgc ctcagcacag aatgctaagg
    10261 ctgcggtgga ccgccattca agccgagcga agcaagtttg ccctgccgaa actcttctcc
    10321 ggtctctcgc atcgcgcgaa gtattgccgg ccagtcattc agatccgtgg caaagatctt
    10381 cgcaaccatg cgaacggtaa gctcagggac caggcggttt agcgtctctg gctgataacc
    10441 catctcgtcg gctaaccgcg cccagtacag attcgtcgaa tcccacgttt cggcaaacag
    10501 ccggctggag tagcccgcaa acataggaaa aagtcttagg ttcagtaatt ctcgggaata
    10561 agtccgcgcc agtgtggggt gcggcacgcc aaaatcctgt gatagtcggt cccaactgag
    10621 ttccgctggg ttgtcgcggg ctagtttctc taattcccgc cctgccggcc cccaggaact
    10681 ggtatctccg gggaattttt gccggaactc cgcggtcaaa tagaagctgt cagcgggcgt
    10741 gatcatcttc gccggatgcc cggctcgcag ggtatcttcc agttgcgcca acctctgagg
    10801 aggcattcgg tcggaaagga gggtcattac tttaccgcgc aaatcctcat tttccacgga
    10861 ggctgctatg agttcctctc ctgcgcgctg gtagagtgcg acggcgtgca gctcgttccg
    10921 gctcacgccc caccagcgtg gcaaaacggc gccggttaac acggacggca ccagatctga
    10981 ccaaatcagc gcctgtacgt tttccggcgc aatgaaatcc tcctccattt ttgctaggac
    11041 gtaaggcaag tttgcgagcg atccaacgag gtgagcgcca cctcccgccg gagatcctgc
    11101 cccgaataga cgcggcgttt gccaaagtga tccttccaat cccatgatgg tctctcccga
    11161 gaaatcatgc gatcgaatca cgagggagtt gttgtgaaga gcctgggcgc ccgggggctg
    11221 gtagtaggcg tagctgaaac ccaccagcgt gtccctgagg aacggagcaa gttgtcctcg
    11281 agcttcttcc aactgcgctc tggaagcagg tgcctgcaaa atctttgcaa catcggtccg
    11341 catttctaat tcggtatgcc gattgttgta agttcccgcg gtaaactccg ttctctcgcc
    11401 gcgagtgaag atcggctgtg gcatttggaa ttccgtcagt tgctcggcga gaggaacgag
    11461 cttatcgccc gtagcgcttc ctggagccgc ttgcgccatc gcagtcagac cgtcgccaag
    11521 ctccagcagg gtatcgagag aaacgagccg ctgatcgtct aggaccgcgt gaatcctttc
    11581 ggccaattcc ttgtggattc gctggccctc ccggctggtt tggcgaggac ccgccagcaa
    11641 atcaacgatt tcatcttggg agctttgtgg ttcgccagta gcagcgcgca gcacttctcc
    11701 cagtgacgtg tgtccggcat cgaaaagctg cgtagctgaa gacacgtggc cgaaaggctt
    11761 gaccattgcc tgccaagaac cgtcctgttt atttgctgca atctctccct ggcgcgaaag
    11821 gatttgccag aggctaacgt tcgcctcaaa aattcccatc gcgttcccac gcagcgtgtg
    11881 ttcgcgaatt ctctcgagcg ccgaggctgt cctcaaaaat tccgcgatgg aggcgtcgct
    11941 caattcggga aattcggaaa agatcaaata ctgatccttg aattcagcgt acctgcccgc
    12001 catcagttca accgcttggg gccccaagcg acgccccgcg ggccgcctgc tatcgagttc
    12061 gttcagcgcc agaaaaattt ccgttggccc cccttcctcg cgcactcgag aaaaagcaaa
    12121 cagggcttcc aggagcccct ccggatcctt caggcttctg gcccgtatgc ccaagttacg
    12181 agtagctttg gaagaggtct tttgaccaaa gatgcgttcc caagcttgga gatttccagg
    12241 cacgtgtggc tcgccatcgg gttcccactg aagacgcgtc atcaacacca ggaggtccgg
    12301 gtcagggcga aagactgacg ccatggcgcc gggcagcgcg tcctttcccc gaagcgcctc
    12361 gtagcaacgt tgcagtcgag ggggttcagc gaaatgcgct tgttcttccg gagggatacg
    12421 cgagagtgaa tcgaagtacg ccgcaagcca accgttgtcc tttgcgagca gtcggggcac
    12481 gaattcggac ggtgactgtg ggcttgaccc caccaggttt ttccatgctg attcggcgcc
    12541 tgaacccccg ggcaccacca cgtgtcccga ttgaacgcgg agatagcttc cataaaagtc
    12601 cagcgagtcg gccaaaggct ccagtctttt cagacctggc gattgccgga gagttgttcg
    12661 aatctccggg tctatgtgag cccaggcgta gtaaaggtgt gccaggacag gatcgtgcaa
    12721 aagcgcatca agcaagtctc ggccgttttc gcctttgcca ccatgctcgc ttgccgccgt
    12781 ccagtcctgc tccgttagca atatgggtac
//
LOCUS       DATNDT010000393         3939 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_665689, whole genome shotgun sequence.
ACCESSION   DATNDT010000393 DATNDT010000000
VERSION     DATNDT010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_665689"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..119)
                     /locus_tag="VN788_12810"
     CDS             complement(<1..119)
                     /locus_tag="VN788_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HXU21455.1"
                     /translation="MIGGGLAGLASAVALAEAGIEVQLFEKRPHLGGRATSYTL"
     gene            complement(70..2010)
                     /gene="hpnC"
                     /locus_tag="VN788_12815"
     CDS             complement(70..2010)
                     /gene="hpnC"
                     /locus_tag="VN788_12815"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR03464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HXU21456.1"
                     /translation="MLAAPELEITRNLPPEGCSVQEAQRYTRWLATHHYENFAVVSWL
                     LPRHLHQHFYNIYSYCRWSDDLGDEVPDRARALQLLDAWENDLRRIYQPGSSQSHPVL
                     IALRETVRAKDIPIKPFSDLLRAFRQDQTVQRYATWESVLDYCVYSANPVGRLVLYLC
                     GYRDAERQRLSDYTCTALQLTNFWQDVSRDLEKGRIYIPLNALAEHNLTDTDIVERRF
                     DARYAALMEELISRTREMFLGGLPLIKIVDGPLRTDIELFSRGGLAVLDAIEAVGYNT
                     IEHRPALTKSTKLRLLTRSLGSRIFSHTTVKDTAAPLSNASSAFPSVSAGDVKSATPA
                     QPADAESVAASYVECNRIARAARSSFYLAFFGLPKQKRNALCALYAFMRLVDNISDDP
                     GDLDSKRRGLARWRALLDEAVEGHTEGHAILPGLADTIAHFEIPTRYFHDLILGAEMD
                     LTVGSYATFDRLSEYCYRVAGTVGLTCLHVFGSRDPRAPDLAERLGLAFQLTNILRDV
                     RSDFEMGRVYLPQEDLDRFGVAPVELGGPLTPRVQGLLSVEAARAWRFYEEGAPLVDK
                     VDADSRATLWALIRTYSSLLARIEERGFDIFSARVTLPSTEKLQHLLAAGMTGWWKRD
                     ALAKRTGDRRRSGGISLRRRAG"
     gene            complement(2158..3225)
                     /locus_tag="VN788_12820"
     CDS             complement(2158..3225)
                     /locus_tag="VN788_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HXU21457.1"
                     /translation="MSKLEQNSLMTAAVLYGSEDIKIERVDIPRLLEDEVLVRVEVAL
                     TCGTDLKVWKQGFHARMIQPPALFGHELAGIVEEIGSEIKGSFRKGMRVVPANSAPCG
                     ECFYCRKSQSNLCEDLLFNNGAYAEYIRIPGRIVRENMLEIPEGVSFLDAAMAEPLAC
                     VLRGIHETHIQSGDTVVVIGCGPIGLEFIRVLSQRDVRVIAVARRPARKLAAERMGAA
                     VVFDVSEGAVEDVVGQVKRVTEGGRGADSVIEAAGQPQTWQWALEMVRKGGAVNLFAG
                     CAKGTEVKVDPWALHYSEITVKSTFHHTPRFFREALDTIARGEIRARDFITGEAPLAD
                     LPSVFEHMKNRNGELKTAVIP"
     gene            complement(3222..>3939)
                     /locus_tag="VN788_12825"
     CDS             complement(3222..>3939)
                     /locus_tag="VN788_12825"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXU21458.1"
                     /translation="AGFDPNGGGFAEYVRAMPWIVEGGMIALPRDVTFEEATFVEPVN
                     TCLKAIRKARVAAGETVLVIGQGPIGMLLAILSKHEGARVYTSDPMAGRRAASLRFGA
                     AESFDPGASSLQAEIRRRTGSRGADAVLLAAPSPALVPEALSIARPGGRVLLFAHNDP
                     VLQLNFPAAAVGIEEKEILGSYSASLDEQEESSALVFSRKLPLAQLISHRFPLERIAD
                     ALDLAAHPKQDTLKVAITHE"
BASE COUNT          874 a         1143 c         1134 g          788 t
ORIGIN      
        1 agcgtgtaag aagtggcgcg ccctcctaag tgcgggcgct tttcgaacaa ttgaacctcg
       61 ataccggctt cagccagcgc gacggcggag gctaatcccg ccagaccgcc gccgatcacc
      121 agtacgcttt gcgagggcat cccgcttcca ccaacccgtc atacccgctg cgaggagatg
      181 ttgaagtttt tccgtgctcg gcaaagtaac ccgcgcggaa aagatgtcga atccccgttc
      241 ttcgattcgt gcaagaaggc tgctataggt ccgaatcaac gcccacaacg tcgcgcggct
      301 gtccgcgtcg accttatcca caaggggcgc gccctcttca tagaagcgcc acgcacgggc
      361 ggcctcgact gacaaaagac cctggacgcg cggcgtcagc gggccgccca gttcgacggg
      421 tgctacgcca aagcggtcga gatcctcctg cggtaggtac acgcgaccca tctcgaaatc
      481 gctccgcacg tcacgcagga tgttagtgag ctgaaacgca agaccgagac gctcggccag
      541 atcaggcgcg cggggatcgc gagagccgaa aacgtgcagg caggtaagcc ctaccgttcc
      601 agcaacccga tagcagtact ccgagagcct gtcgaatgta gcatacgacc ctaccgtcaa
      661 gtccatctcg gcaccgagaa tcagatcgtg gaagtaacgc gtaggaattt cgaagtgcgc
      721 aatcgtatct gcgagcccgg gcagaatggc atgcccttcg gtatggcctt cgaccgcctc
      781 gtccaggagt gcccgccacc gcgccaaccc gcggcgtttt gaatccagat cgcctggatc
      841 gtcggaaata ttgtccacca ggcgcataaa ggcgtagagc gcgcaaagag cattacgctt
      901 ttgcttgggc aagccgaaaa aggcgagata aaagctgcta cgcgccgcgc gcgcaatacg
      961 attacattcc acgtaggatg cggcgacgga ttccgcatcc gccggctgag cgggcgtggc
     1021 cgattttacg tcgcctgcag aaacagaagg aaatgcggat gacgcgttcg acaatggggc
     1081 agcggtgtct tttaccgtcg tgtgcgaaaa aatcctggac ccgagcgatc tggtgagcaa
     1141 ccgcaatttc gttgatttgg ttagcgcggg acggtgctcg atcgtgttgt atccgaccgc
     1201 ctcgatggcg tcgaggacgg ccaagccgcc acggctgaac agttcgatat cagttcgcag
     1261 cgggccatcg acgatcttga taagcggcag accgcccaga aacatctcgc gcgtgcggga
     1321 gattagttcc tccatgagcg cggcatacct tgcgtcaaag cggcgctcga cgatatccgt
     1381 gtcggttaga ttgtgttcgg cgagcgcgtt cagggggata tagatgcggc ccttttctag
     1441 atcccgcgac acatcctgcc aaaaatttgt caactgcagc gcggtgcaag tgtaatcgga
     1501 gaggcgctgg cgttcggcat cgcgatagcc gcaaaggtac agcacgagac ggcccaccgg
     1561 attggcggaa taaacgcaat aatcgagcac ggattcccat gtcgcgtagc gctgcacagt
     1621 ttggtcctga cggaatgcgc ggagcaagtc gctaaacggc ttgatgggaa tgtccttcgc
     1681 gcgcacggtc tcgcggagcg caatcagcac gggatgagac tgcgaactgc ctggctgata
     1741 aattcggcgg agatcgttct cccaggcgtc gagaagctgt aatgcgcgcg cacggtccgg
     1801 cacttcatcg ccgagatcgt ccgaccagcg gcaataggag taaatattat agaagtgctg
     1861 gtgcagatgg cgcggcagca gccaagagac gacggcgaag ttctcgtagt ggtgcgtcgc
     1921 cagccagcgg gtatagcgtt gggcttcctg tacgctgcat ccttccgggg gaaggttgcg
     1981 ggtgatctcg agttctgggg ctgcgagcat cctagaagtg gcggttagac ggccatcttt
     2041 tgttgcaata gcttaatcgc gcttccgaat cggcggtgag gtacgattag aacgttgccc
     2101 ctttttggcg aaacgcgacc aaaaaatacg aaacacgctt ataacgctag atcgctatta
     2161 tggaatgacg gcggttttta gttcaccgtt gcgattcttc atgtgttcaa acacgctggg
     2221 aagatccgca aggggcgctt cgcccgtaat gaaatccctg gcgcgaattt ctccgcgcgc
     2281 gatggtgtct aacgcctcgc gaaaaaagcg cggcgtgtga tggaaggtgg acttcaccgt
     2341 gatctcggag taatgcagcg cccatgggtc gaccttcact tcggtgccct ttgcgcagcc
     2401 agcaaagaga ttcaccgcgc caccctttcg gaccatttcc agcgcccact gccaagtctg
     2461 cggctgaccg gcggcttcga tcacggaatc cgcaccacga ccgccttctg tcactcgctt
     2521 gacttggccg accacatctt cgacggcgcc ttcggaaaca tcgaacacga ccgcagcgcc
     2581 catgcgctct gcggctagct tccgagccgg acgccgcgct acggcgatca cccgcacatc
     2641 gcgctgagac agaacgcgaa tgaactcgag accgatcggc ccacaaccga tcacgaccac
     2701 agtatcgccg gactgaatat gcgtttcatg aatcccgcgt agaacgcaag ccaatggctc
     2761 agccatggcc gcatccagga aggacacgcc ctcgggaatt tcgagcatat tttcgcggac
     2821 aatgcgtccc ggaatgcgaa tgtattcggc ataggcgcca ttgttgaaca gcaggtcctc
     2881 gcaaagattg gactgactct tgcggcagta aaagcattcc ccgcagggag cagagtttgc
     2941 cggtaccacg cgcatgcctt tgcggaaact tcctttgatt tcgcttccta tttcctcgac
     3001 gatgcccgcc agttcatgac cgaagagagc gggcggttga atcatgcgcg cgtgaaagcc
     3061 ttgcttccag actttgaggt cggtaccgca agtaagagcc acttcaacgc gcaccaatac
     3121 ttcatcctcg agtaagcgag gaatatcgac gcgctcgatt ttgatgtcct ctgacccgta
     3181 gaggaccgct gcggtcataa ggctgttctg ttcgagttta ctcattcgtg agttatcgcc
     3241 accttaagag tatcctgttt tggatgcgca gccaaatcca gagcgtctgc aatgcgctcg
     3301 aggggaaacc ggtgcgatat taactgggcc agcggtaact tcctactgaa gaccagcgct
     3361 gaagattctt cctgttcgtc cagggaagcg ctatagctgc caagaatttc tttctcctca
     3421 attccgactg cggcagcggg gaaattcagt tgtaggaccg ggtcgttatg agcgaaaagc
     3481 aggacgcgtc ctccaggacg tgcgatcgag agagcctccg gaactaatgc tgggctgggc
     3541 gcggcgagaa gaaccgcatc cgcgccgcga cttcccgtac gacgccttat ttctgcctga
     3601 agagagcttg cccctggatc gaatgattcc gcggcgccaa atctcaagct tgcggcgcga
     3661 cgccccgcca ttggatcgct ggtatagacg cgcgcacctt catgtttcga aagaattgca
     3721 agtagcattc caatcgggcc ttggccgatc actagaactg tttcgccggc tgcaacgcgg
     3781 gccttgcgaa tggctttcag gcaggtattc accggttcga cgaaagtggc ttcctcaaag
     3841 gtcacatcgc gcggaagcgc tatcattccg ccctcgacaa tccaaggcat agctcgaaca
     3901 tattcggcaa agccgccccc attgggatca aaacccgca
//
LOCUS       DATNDT010000394         3571 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3430504, whole genome shotgun sequence.
ACCESSION   DATNDT010000394 DATNDT010000000
VERSION     DATNDT010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3430504"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..1369)
                     /locus_tag="VN788_12830"
     CDS             complement(149..1369)
                     /locus_tag="VN788_12830"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HXU21459.1"
                     /translation="MQSFSELPISDYTKHSLASANFSIPTPVQAAAIPHALAGKDVLA
                     TAQTGTGKTLAFLIPVMEQLLQQKVSGIAALALVPTRELAMQVVEQYNALRGKQLPPA
                     ALVVGGLSEGQQITALRRGARLVVATPGRLEDFLDRKLVNFSALRVLILDEADRMLDM
                     GFLPAIRRIASVLPRNRQTLCFSATLEASVAHLVHDYMRQPVRVALGSTLKPSENVRV
                     QAFEVASDHKQEFLERLLAKHIGRCLIFTRTKRGAERLALSLSREGFSAAAIHGDRSQ
                     SQRNAALAGFQQGRHRILVATDVASRGIHVQDIAHVINYDLPELAENFIHRVGRTGRA
                     GAQGLASTLFGADQRGELLALERTLGIKIERMKAADLPERKDRKSAAMHRALAPSSSR
                     ISRLPGEFLQAQTM"
     gene            1712..2089
                     /locus_tag="VN788_12835"
     CDS             1712..2089
                     /locus_tag="VN788_12835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007467486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HXU21460.1"
                     /translation="MPATSQKVFVGTGEKVPEGGRLVVDVGETTIGIFRVDGKLYAYL
                     NRCAHQGGPVCQGKIIPRVTEVIDGGGESHGFAFDESSPHIVCPWHGFEYDIKTGVHP
                     GRPVARLIRVAVEEAADGIYVNV"
     gene            2076..2384
                     /locus_tag="VN788_12840"
     CDS             2076..2384
                     /locus_tag="VN788_12840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21461.1"
                     /translation="MSTSEKAARLSALVAQLSEGIDETVKAGEVSDVPRENVTALVSG
                     AVKLYATSAEEADSDVPVVDATVSTTEAMVVACALLRAHHLNPFDLALWFSRTARNPH
                     "
     gene            2426..>3571
                     /locus_tag="VN788_12845"
     CDS             2426..>3571
                     /locus_tag="VN788_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21462.1"
                     /translation="MAKTDVANGVSSATEHKVQQITTRTDSREILANARQDIERYHLD
                     DYFIVDVDAHHVEFDSWSEILQRLENPVLRHNAISMTQNWPNAKQLAFSNHPVGLTFQ
                     DVFGRIPHQAELGEYVEKTDEHRDLTLVRRAMDSMGIKMQVVFPQPMLEMGLHPEPDI
                     VTAMTFAYNRWFTDTILPREKRVKSLLCMPFHDPDACMKTIQEFGEKQGVVGFMITSQ
                     RHVGVHKNMYMRVYKELEERGLPLAFHAGPTWGDTMTSTMNRFLAVHSMSFVTCNMTH
                     MANWIVNGLPERFPKLKVIWIESGLAWVPFMMQRLDHEYLMRQSDAPLLKRLPSEYIA
                     DMFFTSQPLEVTMPHMLEATFKAVRAETQLMYSSDWPHWDFDVPARIA"
BASE COUNT          778 a          995 c         1077 g          721 t
ORIGIN      
        1 gccctgaaac atctcatgaa agagcagaaa aggttttacc gcgaccgagg caggtagggc
       61 gcagccctct gcgcccttac ctgaaacagt cgtcaaagct ttcctgtaac ggccctcact
      121 tcggtatcgc gcattgtggg ggagccgctt acattgtttg cgcttgtagg aattctccgg
      181 gaaggcggct tattcttgaa ctggaaggcg cgagagcgcg atgcatcgcg gctgacttcc
      241 gatcttttcg ctccggcaga tcagccgctt tcattcgctc aattttgatg ccgagtgtgc
      301 gctccagcgc gagcaattct cctcgctgat cggctccgaa aagagtggag gcaagaccct
      361 gtgctcccgc gcggccggtt cggccaacgc ggtgaatgaa attctccgct agttcgggga
      421 gatcgtagtt aattacgtgc gcgatgtcct gcacatgaat gccgcgggag gcgacatccg
      481 tggcgacaag aattcggtgg cggccttgtt gaaagccggc caaagcggcg ttccgctgcg
      541 attgcgagcg gtccccgtga atcgcggctg cggagaaacc ttcgcggctc agactcaggg
      601 ctaggcgttc ggctccccgc ttggtgcgag tgaagatgag gcagcgcccg atgtgcttgg
      661 cgagcaagcg ttcgagaaat tcctgtttgt gatcggaggc aacttcgaat gcctgcaccc
      721 gtacattctc ggacggtttc agcgtcgagc cgagcgccac gcgcaccggt tgccgcatgt
      781 aatcgtgaac cagatgagcg acggacgctt cgagcgttgc ggaaaagcaa agtgtctgcc
      841 gatttctcgg cagcacggat gctatgcggc gaatcgcggg aagaaatccc atgtccagca
      901 tgcggtccgc ttcatcgaga atgagcacgc gtagggcgga gaaattcaca agcttgcgat
      961 cgagaaaatc ctcgagccgg cctggagtgg caaccacaag ccgcgccccg cggcgcagcg
     1021 cggtaatctg ctggccttcg gaaaggccgc cgaccacgag agcggcgggc ggaagctgtt
     1081 tgcctcgcag cgcgttgtat tgttcgacga cttgcatggc gagctcgcgc gtgggaacaa
     1141 gagcgagagc ggcgattcct gaaaccttct gctgaagcag ttgctccatc accggaatca
     1201 gaaatgcaag cgtcttgccg gttccggttt gcgcggtggc aagcacgtct ttgccggcaa
     1261 gcgcgtgcgg gatcgccgct gcttgcacgg gggtggggat tgaaaaattg gcggacgcca
     1321 agctgtgctt ggtgtagtcc gaaatgggta attctgaaaa actctgcatt gtctaagtgt
     1381 tttctgttgg aactgcgcgt tgcggcgcgc cggataaact ccgagcgacc agctttagct
     1441 gcagttccat tccttgtttt aaattacgct gaagacttcg gaagggacta aaggcggggc
     1501 attctcagca gataaccgac gagagcaacc acaccacaca accgctgtat taagcgcaac
     1561 gaaagtatac cacaggcgcg caatcttgat aaggcgtggt tctcaacata caatagccgc
     1621 gatttgggta agcgtgcctg gtggcgcggt tgcggcgagc ttcacgcgga aggtggccgc
     1681 ttacttttac gcgcagctag ttggagacca tatgccagcc acatcgcaga aggttttcgt
     1741 gggcaccggc gagaaggtgc cggaaggcgg gcgcctggta gttgatgtcg gcgagacgac
     1801 gattggaatt tttcgcgttg acggcaaact ctacgcgtat ctcaatcgct gcgcgcacca
     1861 aggcgggccc gtttgccagg ggaagattat tccgcgcgtt accgaagtga tcgacggcgg
     1921 cggggaaagc cacggattcg ctttcgacga atcgagcccg catatcgttt gcccgtggca
     1981 cggcttcgaa tatgacatca aaaccggcgt gcatcccggg cggcccgtgg cccgcttgat
     2041 ccgagtggca gtggaagaag cggctgacgg gatttatgtc aacgtctgag aaagcggcgc
     2101 ggctgtcggc cctcgtcgcg caattaagcg aggggattga cgaaaccgtg aaggcgggcg
     2161 aagtctcaga tgtgccgcgg gaaaatgtga ccgcgctggt gagcggcgcc gtgaaacttt
     2221 acgcgacctc cgcggaagaa gcggattcgg atgtgccggt ggtcgatgcc acggtttcga
     2281 ccaccgaggc gatggttgtt gcgtgcgcct tgcttcgcgc gcaccatttg aacccattcg
     2341 atctggcgct atggttttcg cgcaccgcgc gaaatccgca ttgaagagtc gtgaagtagc
     2401 taacattgtt agcggggata ggacaatggc caaaaccgac gtagcaaacg gcgtgagcag
     2461 cgccacggaa cacaaagtgc agcagatcac cacccgcacc gactcacgcg aaatactagc
     2521 gaacgcgcgc caggatatcg aacgctacca cctggacgat tatttcatcg tggatgtgga
     2581 cgcgcatcac gtcgaattcg attcgtggtc tgaaattctg cagcgtctcg aaaaccctgt
     2641 gctgcgccac aacgcaatct cgatgacgca gaattggccc aacgcgaagc aactggcgtt
     2701 cagcaatcat ccggtcggcc tcacatttca ggacgttttt ggccgcatcc cgcaccaagc
     2761 cgaactcggc gaatacgtgg agaaaacgga cgagcatcgc gatctcacac tggtgcgtcg
     2821 cgcaatggat tcgatgggaa tcaaaatgca agtggttttc ccgcagccca tgctggagat
     2881 gggcctgcat cccgagcccg acatcgtcac ggcgatgacc tttgcctaca accgctggtt
     2941 cacggacacg attctgcccc gcgaaaagcg cgtgaaaagt ttattgtgca tgccgttcca
     3001 cgatcccgac gcgtgcatga agacgattca agaattcggc gagaaacaag gtgtggtcgg
     3061 cttcatgatc accagccagc gccacgtagg cgtccacaaa aatatgtaca tgcgcgttta
     3121 caaagagctc gaagaacgcg gcctcccgct cgcgtttcac gccggcccca cctggggcga
     3181 cacgatgacg tccacgatga atcggtttct agcggtgcat tcgatgtcgt tcgtcacctg
     3241 caacatgacc cacatggcga attggatcgt gaacggctta cccgaacgct ttcccaagtt
     3301 gaaagtaatt tggattgaga gcggcctggc atgggtgccg ttcatgatgc agcgactcga
     3361 tcacgaatac ctcatgcgcc agtccgacgc gccgctcttg aagcgcctgc ccagcgaata
     3421 catcgccgat atgtttttca cctcgcagcc gctcgaagtg accatgccgc acatgctgga
     3481 agcgacgttc aaagccgtcc gcgcggaaac gcagctgatg tattcctccg attggccgca
     3541 ctgggatttc gacgtccccg cgcgcatcgc a
//
LOCUS       DATNDT010000395        12753 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3311733, whole genome shotgun sequence.
ACCESSION   DATNDT010000395 DATNDT010000000
VERSION     DATNDT010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12753)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12753)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12753
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3311733"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VN788_12850"
     CDS             complement(<1..381)
                     /locus_tag="VN788_12850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXU21463.1"
                     /translation="MGIPSVPVITKAFKNLVILNAAKRGMPHERITFTPHPVWGKTPA
                     ELRAYVNGPDPVSGKPMMKEIVASLTTPLTEDEKKVGMVSVSIGPPTFTDTLDNLQEF
                     YMNNGYTDFMPIIVPTEEKVETMLK"
     gene            complement(430..840)
                     /locus_tag="VN788_12855"
     CDS             complement(430..840)
                     /locus_tag="VN788_12855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21464.1"
                     /translation="MNRRKLLSLLGMSPILLTTDSAKAESLSKNTKTASKTGSTGGKV
                     TAVNPKGTPPAIKLIPMAPRLDSLDGKTVYLVDTGFHGSDTLLDQMQIWFKENMPDVK
                     TVFRRKAGPYAQDDPALWKEIKEKGNAVIMAVGH"
     gene            complement(1150..2262)
                     /locus_tag="VN788_12860"
     CDS             complement(1150..2262)
                     /locus_tag="VN788_12860"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HXU21465.1"
                     /translation="MRERQILSIMALVVALAIGCAKKVDDSALVTKIQSQMFSDSQLK
                     DANLQVASNKGAVTLTGTVSSTAAHLDAYKIATQTPGVASVNDQITVQEPQATQAAPA
                     PAPAPAEAQSPAKTKQERRREAREAREKKHHARPSTEEVAENVPQNPDPAPQQAAPQS
                     APQEQTPPPPAEPAAAPAPEPTPPPPPPPQPRPVRIDAGTTVTIRMIDGIDSSVNQPG
                     EIFHASLDAPLVVGNEVVAPKGTDVYVRLVSASQAGKFNGKSELRLELIKMELHGQSY
                     PLISSAYSLAGSSRGKNTAEKVGGGAVLGAIIGAIAGGGKGAAIGAGVGGAGGGVYQA
                     TTHGKEVKIPSETKLDFQLDQPVTIMVMPRPTPENQ"
     gene            2493..3203
                     /locus_tag="VN788_12865"
     CDS             2493..3203
                     /locus_tag="VN788_12865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU21466.1"
                     /translation="MRIRSLAALLVLALFCVALGNRAPARADAEKKNVTAEIKSLKAA
                     VAQLQKQVQNSQDFIAISNLQAAYGYYVDKCEWDQTADLFAKDGTLEIGLRGVWVGQD
                     RVRAYLHTLPQLKYGTLFNHMQLQPKIDVAPDGSTAKARFRAFEQFGMLHRSAQWAEG
                     TYENEYVKEEGVWKIKKLHYYMTYYVDFYKGWDQGGNPPPGPIAGFPPDQPTTVPYKL
                     YPDVFVPPYHYTNPVTGK"
     gene            complement(3632..6466)
                     /locus_tag="VN788_12870"
     CDS             complement(3632..6466)
                     /locus_tag="VN788_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HXU21467.1"
                     /translation="MSKEMVISANPHETRVAILEEGQLCEYYVEREKEFALVGSIYKG
                     RVTRVLPGMQSSFVDIGLDSDAFLYVTDFLEGIEELDEHVETAGEKIPPAMDHAGPPN
                     APGETAEHASEIAAGDTSFGASEVESHEPGNLQEPAPPMPAVTPAPPLNTAQPDRPRY
                     ESRNRQGRDFGQRGGRRGGRFGRGGRGGGRGGGGRYGRELPQSKYASPRTYEDRPGEA
                     GDAPEGHVSVILPGESLARYKDKPTTENPPAAAPTEQTIGTVDEQAGRSSALADSAES
                     VTETAATSSASEFRPAHHENSRPAYPPSSSSGLEPLPGESLSKWKQRAPEIQEPSESE
                     SMGASAFHEDRELPSEPEMADLLHPDELHEMQQTAGLSEDEASALAEHVAEAQSEQAE
                     REAQVRGFGATDTAAEATDELVGTETDDGEAEAQSETESEAEEVQLEQEEADAEAEGM
                     IAPPAELGNEAEHELAVPPEAIAAESVSPTALENSQPQSQQPWKARIHDDFRPRMQHP
                     ARRGGRDRGRRDDRGRRPQRGHGGHGGHHHAQARRPQLIADLLKQGQEIIVQIAKEPL
                     GKKGARITSHVALPGRFLVYMPTLDHTGVSRKIASPEERARLRHLVNDAKGSAGGGFI
                     VRTAAGSAAPEEVRADVEFLTRAWSEIKGRSEQRKAPSLLHRDLNLVERMLRDHISSD
                     YTAIWVDSEEEYTKVVDFMGRFQPTLVSRVKLYTKETPVYEEFGIQQEIDKGLRPKVW
                     LKSGGYIVINHTEALVAIDVNTGKFVGKGSNRLEDTIVRTNLEAVKEIVRQMRLRDLG
                     GIIVIDFIDMEERRNREKVMAALEDALRADRAPSKVLRFNEFGLVAITRKRTKQALER
                     TLCQPCPYCTGSGMVKSVPTLCYEIQTEARKMAPEMESGSITLRVHPEIAKALKTRES
                     SLIEELERWTRKSVIIQADPTLHWEQYDIY"
     gene            complement(6719..7819)
                     /gene="rodA"
                     /locus_tag="VN788_12875"
     CDS             complement(6719..7819)
                     /gene="rodA"
                     /locus_tag="VN788_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HXU21468.1"
                     /translation="MKNRPHVQDFDWILLAIVGAISAVGVLEIYSSTHASAMAGMQWK
                     QLMWIGVGILGMFLISRVDYHSLMDQAPLLYLVGVAGLLVVLVLGYSRLGAKRWISLG
                     GLVNLQVSEIMKLIIIIVLARYFAEVHTDRLTLTDLAKISALTLIPVVLILKQPDLGT
                     ALVLTPVAVVGAFFAGIEWKHAAVGLLVLALLIPLGWNMRRHLKPYQQQRIQTFLHPE
                     QDQRGAGYQILQSEIAVGSGGFWGKGFGKGSQNQLGFVPVRYSDFILAALAEEQGFIG
                     VCVVLLLYLGLILRLVDNAQKAKDRAGMYVVMGVTAILGFHVLVNASMVIGDVPVTGI
                     PLPLMSYGGSATAFVFLALGLVMNVRMRRFVN"
     gene            complement(7816..9672)
                     /gene="mrdA"
                     /locus_tag="VN788_12880"
     CDS             complement(7816..9672)
                     /gene="mrdA"
                     /locus_tag="VN788_12880"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HXU21469.1"
                     /translation="MAGWNVNDRKIPQGRLALFSYLSVTFVIFLLVGFWKLQVVQSSH
                     YSDLADRNGIRSIAIIAPRGAMLDRDGRVLVDSYPSFSILLLRDNPKLIEKALPQIED
                     GLGISKEDLRQQLDAAKNEPKFLPVIIKPAATEGDIAFVESHRADLPVLDLMMVQRRR
                     YPHGEMLANTIGYVGEVSPQDLEKNPDRYRPGDIVGKAGLEKEYNDQIVGTDGMRRVV
                     VNSVGKVMRTLDNVDAIPGKPIQLTIDEDLQQIAETDFANKEGALVAMDAHTGEVLAM
                     VSRPTFDPNDFSVRIPNKEWAQLNTDPKTPLINRAIQAQLAPGSVFKVVMATAMLESK
                     ALPANYTVYCPGYATFYGRVFHCDHPHGSVDLHKGIVASCDVFFYNVGKILGIDRIDH
                     YATGLGLGRRTGIDLPGEEPGLIPSEAWVERVGHHKWYPGSTISVSIGQGAVMATPIQ
                     LARMIAAVATGGTLIPPHLVKNATGLKAESFPISEDTVQQVTDGMWGVVNEPDGTTSG
                     LVKLQNIDFSGKTGTAQVESYDLQKKLGKKLKENGWFVGYAPRRNPDIVVAALVQAGG
                     WGSTSAAPIVRDVVKAYYDKKNGHLPAPPSTAEVIRPSGMKPVAENSGAARP"
     gene            9963..10454
                     /locus_tag="VN788_12885"
     CDS             9963..10454
                     /locus_tag="VN788_12885"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HXU21470.1"
                     /translation="MQINISGWRKATLLCLACALFLLIAPMAHAQQTARAKALGAQLK
                     CVCGCNQVLTACNHVGCTYSHAMLKELDERVTRGDSDSLILQSFVQQYGEVVLAEPPR
                     KGFNWLVWILPIAAPLLAFFMIWEVVRRWRHRSVLAPAEGPGLSSDLLDRARRESGEN
                     EIE"
     gene            10447..10779
                     /locus_tag="VN788_12890"
     CDS             10447..10779
                     /locus_tag="VN788_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21471.1"
                     /translation="MSNLDIAVACILLALALMFFVFGVRWEPSDSAPHRTKLDQLMER
                     RDAIYENLRDLRFERRAGKFSEKDYDEIKSSLEIEAARVLADMDTLTGGAPTPATRQA
                     ASAKGPRS"
     gene            10797..12086
                     /locus_tag="VN788_12895"
     CDS             10797..12086
                     /locus_tag="VN788_12895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21472.1"
                     /translation="MTKWLALASLFVFLASPAFAGTVTGTVTNGTTGKPAAGVEMILI
                     QLQGTMKPIANTKTDSNGHYSFDNPALGAGPMLIRAVYRGVNYHEPATPGKTTVDVQV
                     FEPTDKASAFSVTAHAIILQPSGSTLVVGEEYNISNKTQPPVAYYRPNGSFSFALPDG
                     AQLTDVSVVGTSGMPVVQTPVDEGNSKQAIDFPFRPGDSVVRISYHLPYPSNQANLRF
                     VSDYSADRLAVFAPPSVHFTGAGFTPGGDAQGFSVYMRESVAAKTPISVSVSGTAPPP
                     APDNGGSSNAAGADDSQNPSVNSHADSGQETPVANITTFPARLDSLKWILVGGFAALF
                     VVGLIYVLRQPEAAVIGTPNEMAAPPLPDLAAPPPQAATAKPPANVDQEVRSSLDQLK
                     DTLFRLELRREAGTIAEDDYIRERDRVQKTLRDLVKG"
     gene            12116..>12753
                     /locus_tag="VN788_12900"
     CDS             12116..>12753
                     /locus_tag="VN788_12900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU21473.1"
                     /translation="MAPSSAPAISAAAALGVSFERVEKRFGSFFALRGVSLEIQTGEF
                     VALLGPNGAGKTTLLRLAALLATPTSGRVRFPGIAPENSPKSLIGMVGHSTLLYDELT
                     AKENLDFFAQLYGIGDAGARVAASLESCGLALRATSLVRTFSRGMRQRLAIARALLHR
                     PRMLLLDEPAAGLDRQGLAWLASTLARLRDSGATVVMSTHARNESLDLATRAV"
BASE COUNT         2449 a         4026 c         3463 g         2815 t
ORIGIN      
        1 cttgagcatc gtttcgactt tttcttccgt cggcacaatg atcggcatga aatccgtata
       61 gccgttattc atgtaaaatt cttgcaggtt atcgagcgta tccgtaaaag ttggcggccc
      121 gatcgaaacc gaaaccatgc caactttctt ttcatcctct gtaagtggcg tcgtcaggct
      181 ggccacgatt tccttcatca tcggtttgcc gctcaccgga tccggtccgt tgacgtacgc
      241 gcgaagctct gccggcgtct tgccccatac cggatgcggc gtaaacgtga tgcgttcgtg
      301 cggcatcccg cgcttcgctg cgttgaggat caccaaattc ttaaatgcct ttgtgattac
      361 gggtacgctc ggaattccca tcttttccgt tgttatgcag tgaccgacgg tcgctggggc
      421 gcaggtgctt caatgcccca cggccataat taccgcgttg cccttttcct tgatctcttt
      481 ccaaagcgcc ggatcgtctt gcgcgtacgg tcccgctttt cgccggaaaa ccgttttgac
      541 gtccggcatg ttttccttga accaaatctg catctggtcg agcagcgtat cggagccgtg
      601 gaaacccgta tccacaagat agacggtctt gccgtccagc gaatccaggc gcggcgccat
      661 cggtatcagc tttatcgccg ggggcgtgcc cttcggattg acggccgtaa ctttgccgcc
      721 cgttgagccg gtcttgctcg cggtcttggt attttttgaa agggattcag ccttcgcgga
      781 atcagtcgtc aaaagaatcg gggacattcc cagcagtgat aagagcttac gtcgattcat
      841 tagttggctc ccgtctagaa tacaaatcag cgccggacac ctttaaggac ggccccgcgc
      901 acgcacaggt taccaccgca tcaacttata gtctcgcccc tgcgggaatt gcaagcgttt
      961 tgcaccggct ctcgatgaca actccgattc ggctccaact ttaaccaagc catccgattt
     1021 tttgctgctt tgcaaacgac ggcccagtgc gaagccaaac ttagatgatc ggagtttttg
     1081 aaatgtgcaa aaaaaaagcg gaaagcgaat cgtcgttcgc ttccgctttt ctgaattcac
     1141 cgaagcgcgt tattgatttt ccggcgtcgg tcgcggcatc accatgatcg tcaccggctg
     1201 atcgagctgg aaatccagct tcgtttccga gggaatcttc acttcctttc cgtgcgtcgt
     1261 ggcctgataa actccgcctc ccgcgcctcc gactcccgcg ccgattgccg cgcctttgcc
     1321 gccgccggca atggctccga taatcgcgcc cagcacggcc cctcctccca ctttttccgc
     1381 cgtatttttc ccgcgcgagc tgcccgccag cgaatacgcg ctgctgatca gcggatacga
     1441 ttgaccgtga agctccatct tgatcagttc caggcgcaat tcgctcttgc cgttgaattt
     1501 tcccgcttgt gacgctgata ccaaccgcac atacacgtcc gtccccttag gcgccaccac
     1561 ctcattgcca accacaagcg gcgcatcgag cgaagcgtga aatatttcac ccggctgatt
     1621 cactgacgaa tcgattccgt cgatcatgcg aatcgtcacc gtcgtacccg catcaatccg
     1681 cactggcctc ggctgcggag gaggaggcgg tggcggagtt ggttcaggcg ccggagccgc
     1741 ggcaggttcg gcgggcggcg gtggagtttg ttcttgcggc gctgactgag gagctgcctg
     1801 ctgaggcgcc ggatcgggat tttgcggaac attctccgcc acctcttccg tagacggccg
     1861 cgcgtgatgt tttttctcac gcgcttcgcg agcctcgcga cgccgctctt gcttggtttt
     1921 tgcaggggac tgtgcttccg caggcgcagg cgcgggagcc ggcgcagcct gggtggcttg
     1981 gggctcctgc acggtgatct gatcgttcac ggacgccacg ccgggagttt gtgtcgcgat
     2041 cttgtaggca tccaggtgcg ctgcagtgct cgaaacagtt cccgtcagcg tcaccgctcc
     2101 cttgttagag gctacctgca gatttgcgtc ctttagttgc gagtccgaaa acatttgcga
     2161 ttgaatcttg gttaccagcg cggagtcgtc caccttcttc gcgcacccaa tcgcgagcgc
     2221 cacaaccagc gccattattg acagtatctg tctctcgcgc atttcactct cctttggttg
     2281 atggcccaga cgagttacgc aactactcta ctacgatgcg tcgtgagtcg ctaagtgatg
     2341 ccgcgatttt cactcctatt ttacgcccgc gagggacatg ctgttgtctc cacgctccgg
     2401 tggatgcgtt ccaatccgcc ttgccaacca caatgctttt cgactaactt ccccatcggc
     2461 gcaatagaca aaaaaaacgg aggcatcttc caatgcgaat tcgctcactc gcagcactct
     2521 tggttttggc gctgttctgt gtggccctcg gaaatcgcgc acccgcgcgc gcggacgcag
     2581 aaaaaaagaa tgtgacggct gaaatcaaat ctctcaaagc cgccgtcgcg cagcttcaga
     2641 agcaggtgca aaattcgcag gacttcatcg ccatctccaa cctgcaagcc gcctacggct
     2701 attacgtcga taaatgtgag tgggaccaga ccgccgacct gttcgcgaaa gacggcacgc
     2761 tagaaatcgg cctgcgcggc gtatgggttg gccaggatcg cgtccgcgcc tatctgcaca
     2821 cgctcccgca gttgaagtac ggcacgcttt tcaatcacat gcagctccaa ccaaaaattg
     2881 atgtcgctcc cgatggctcg actgccaagg cgcgcttccg cgccttcgag cagttcggca
     2941 tgctgcaccg cagcgcgcaa tgggcggagg gaacctacga aaacgagtac gtgaaggaag
     3001 aaggcgtgtg gaaaatcaaa aagctgcact actacatgac ctactacgtc gacttctata
     3061 agggctggga tcagggcggc aatccgcctc ccggccccat cgccggcttc ccgccagatc
     3121 agccgaccac cgtcccctac aagctatatc ccgatgtctt cgtccctccg tatcactaca
     3181 caaatcccgt caccggcaaa tgacgatgcc ggccagcatc atccgcgacg cagttgcagt
     3241 tgcagttgta ggggcacggc atgccgtgcc cggcgcagat gcttggctac cactcagcca
     3301 tcagccgctc tcgtagcgcc gccatcttgg cggcgaattt gcctttcgcg cttgacgagg
     3361 caaaaacgtc gcctggaagc tgccgcctca agaacgtctg gcaatgtcat tccgcttgcc
     3421 ctgacgcagg aagggagcgg ggtgtcccgc gctttttctt ttgcgcgaag cgccaggaca
     3481 cggagtcgag gattttcctg tggactgggg ataaaatcaa agcgccccca aaaacctctc
     3541 ggggcgcctc gaatttccag tttcgaattt gtagtgtccg ctcggcgttg cagtttctaa
     3601 tttctagcct ccaacctcta acctccggtt tctagtaaat atcgtattgt tcccaatgca
     3661 gcgtgggatc ggcctggatg atcacgctct tgcgggtcca gcgctccagt tcctcaatca
     3721 gcgaagactc ccgcgtcttc agcgccttcg caatctccgg atggacacgc agcgtaatgc
     3781 tgcccgactc catctccggc gccatcttcc gcgcttcagt ttgaatctcg tagcacagcg
     3841 tggggacaga tttcaccatg cccgatcccg tgcaatacgg acacggctgg cacagcgtgc
     3901 gttccagcgc ttgcttggtg cgcttgcgcg tgatagccac caagccgaat tcgttgaacc
     3961 gcagcacttt tgacggagcg cggtcagcgc gcaacgcatc ttccagcgca gccatcactt
     4021 tttcacgatt gcgccgctct tccatgtcga tgaagtcgat cacgataatc ccgccgagat
     4081 cgcgcagccg catctgccga acaatttcct tcaccgcctc aaggttcgtg cggacaatcg
     4141 tgtcttccag ccggttcgat cccttgccca caaactttcc cgtgttcacg tcaatcgcca
     4201 ccagcgcttc ggtgtggttg atcacgatgt acccacccga cttcagccaa actttcggac
     4261 gcagcccctt atcaatctcc tgctggatgc cgaattcctc gtaaaccggc gtttccttgg
     4321 tgtaaagctt cacgcggctc accagcgtcg gctggaaacg gcccatgaaa tcgacaacct
     4381 tggtgtattc ctcctccgaa tcaacccaaa tcgccgtgta gtcggagctg atgtgatcgc
     4441 gcagcatgcg ctccaccaga ttcagatcgc ggtgcagcag cgacggcgct ttgcgctgct
     4501 cggatcgccc tttgatttcc gaccacgcgc gcgtcagaaa ttccacgtcg gcgcgaactt
     4561 cttccggcgc cgcgcttccc gcggccgtgc ggacgataaa gcctccgccc gcgctgccct
     4621 tcgcgtcgtt caccagatgc cgcaagcgcg cacgctcttc cggtgaagcg attttgcgcg
     4681 atacgcccgt atgatccagc gttggcatat aaactagaaa ccgcccggga agcgcaacgt
     4741 ggctagtaat tcgcgcgccc ttcttgccca gcggctcttt cgcgatttgg acgatgattt
     4801 cttgtccctg tttgagaaga tcggcgatga gttgcggccg gcgcgcttgc gcatggtgat
     4861 gcccgccatg cccgccgtgt ccgcgttggg gacggcggcc gcggtcatcg cgcctgcctc
     4921 ggtcgcgccc gcctcgccgt gcgggatgtt gcatgcgcgg cctgaaatca tcgtgtatgc
     4981 gcgccttcca cggctgctgg ctctgcggct gcgaattttc tagcgccgtg ggcgagacgc
     5041 tctccgccgc gatcgcttcc ggcggtaccg ctaattcgtg ttccgcttca ttgcctaact
     5101 ctgcaggcgg cgcaatcatc ccttcggctt cagcgtccgc ttcctcttgt tcgagttgga
     5161 cttcttcggc ctcgctttcc gtttccgatt gggcttctgc ttcgccgtcg tcggtttcgg
     5221 ttccaaccaa ttcgtcggtg gcttcggccg cagtatcggt ggctccgaaa ccccgaacct
     5281 gagcttcgcg ctccgcttgc tcgctttgcg cttcggcaac atgctccgcg agcgcggaag
     5341 cctcgtcttc gctcagaccc gccgtctgct gcatttcatg cagttcgtcc gggtgcagga
     5401 gatccgccat ttcgggttcc gacggcagct ctctatcttc atggaatgcc gaagcgccca
     5461 tcgactctga ctcgctcggc tcctggattt caggagcccg ttgcttccac tttgacaagc
     5521 tctcgcctgg cagcggctcc aatcctgaag acgacgacgg cggatatgca ggccgcgaat
     5581 tctcgtgatg tgcaggacgg aactccgaag cgctggatgt cgcggcggtt tccgttaccg
     5641 actccgcgga gtctgccaat gctgaggaac ggccagcctg ttcatccact gtgccaattg
     5701 tttgctcggt tggcgctgct gcggggggat tctcggtagt gggcttgtcc ttatatctgg
     5761 ccaaggattc gccgggcaga atcacggaaa catggccctc aggagcgtca cccgcctcgc
     5821 ccgggcgatc ttcataagtt ctgggcgacg cgtattttga ttgcggcagc tcgcgtccat
     5881 agcgaccacc gccgccacgc ccgccgccgc gtccaccgcg gccaaaacgt cccccgcgcc
     5941 tgccaccgcg ttggccaaag tcgcggcctt gccgattgcg actctcgtag cgcggccgat
     6001 ccggctgcgc tgtattgagc ggaggcgccg gagtcactgc tggcatcggc ggcgcaggtt
     6061 cttggagatt tcccggctcg tgcgactcga cctccgaagc gccaaagctc gtatccccag
     6121 cggcaatttc ggacgcatgc tctgccgtct cacctggggc gttcggcggc ccggcgtggt
     6181 ccattgccgg cggaatcttt tcgccggccg tctcgacgtg ctcgtcgagt tcctcgatcc
     6241 cttcaagaaa gtcggtgacg tacaggaagg cgtcgctatc cagcccaata tccacgaagg
     6301 acgattgcat gcctggcagc acgcgcgtta cacgcccttt gtaaatgctt cctactaacg
     6361 caaactcctt ttcccgttca acgtagtact cacaaagctg tccttcttcc agaatcgcta
     6421 cccgcgtctc gtgcgggttc gcggagatga ccatttcctt cgacatttga aaactcctca
     6481 gggcgcatgc ggatgcggcg gcgcaaaaat gcgcgcgcgc cggcggcggc gtggcgcaag
     6541 ttggtgcagc attttgcctg ccacggcggg ccagtcagga aagatctgaa atgtggctgg
     6601 attgctttcc tcacccgatg cattcctcaa ttcaaaactt aaattacaaa actaaaaatg
     6661 ccaggctccg tttttccaga gcgcggccac taaattttca caacgtactc gacggggttt
     6721 agttgacgaa tcgccgcata cggacattca tcaccagtcc caaagccaaa aacacaaatg
     6781 cggtggccga cccgccgtag ctcatgagcg gaaggggaat accggtcacc ggcacatctc
     6841 cgatcaccat ggatgcgtta accagaacgt gaaaaccgag aatcgccgtc acacccatca
     6901 cgacgtacat cccggctcta tccttggcct tctgcgcgtt gtccaccagc cgcaaaatca
     6961 ggcccaagta caacagcaaa accacgcaaa cgccaatgaa accttgctcc tcggccagcg
     7021 cagcgaggat aaaatctgaa taccgcaccg gcacgaagcc gagttggttc tgactgcctt
     7081 taccgaatcc cttaccccaa aagccgcccg agcctacggc gatttccgac tgcaatatct
     7141 ggtagcccgc gccccgctgg tcttgctccg ggtgcaggaa cgtctgaatg cgctgctgct
     7201 ggtacggctt caagtgacgc cgcatattcc agcccagcgg aatcaacaat gcaagaacaa
     7261 gcaggcccac tgccgcatgt ttccactcga tgcccgcgaa gaaggcgcct actaccgcaa
     7321 ccggagtcag caccaatgcc gttcccaggt cgggctgctt taaaatcaag accactggaa
     7381 taagcgtcaa cgcgctgatc ttcgccaggt ccgtcagtgt gaggcggtcg gtgtgtactt
     7441 ccgcaaagta acgggccagc acgatgatta taatcaactt catgatctca gacacctgca
     7501 aattcaccaa tccgccgagc gagatccacc gcttcgctcc cagccgcgaa tagccgagca
     7561 caaggacaac cagaaggccg gcaactccca ccagatagag cagcggcgct tgatccatca
     7621 gcgaatgata gtcaacgcgg gatatcagga acatgcctag aatcccgacc ccgatccaca
     7681 tcaattgctt ccactgcatg cccgccatgg cgctcgcgtg cgtggacgaa taaatttcca
     7741 gcacgcccac ggcgctgatt gctcccacga tggcgagcaa aatccaatcg aaatcctgaa
     7801 cgtgcgggcg atttttcacg gacgtgcggc tcccgaattc tcagccacgg gtttcattcc
     7861 actcggacgg atgacctcgg cggtgctagg cggggcgggc agatgtccat tcttcttgtc
     7921 gtaataagct tttacaacgt cgcgcacgat cggcgcagcc gaagtgctgc cccatccgcc
     7981 ggcctgaacg agtgcggcca ccacgatatc cggattgcgc cgcggcgcgt agccgacgaa
     8041 ccatccgttt tccttcaact tttttcccag cttcttctgc aggtcgtagc tttccacttg
     8101 agcagtgccc gtcttgccgg aaaagtcgat gttttgcaac ttgactagcc cggacgtggt
     8161 gccatcaggc tcattcacca cgccccacat gccgtccgtt acctgctgga ctgtatcctc
     8221 ggaaatcggg aacgactccg cttttagccc cgtggcgttt ttcaccaggt gcggcgggat
     8281 caacgtgccg ccggtggcca cggccgcaat catccgagcg agctggatcg gcgtcgccat
     8341 cacggcgcct tgtccgatgg aaaccgaaat tgtggagccg ggataccatt tgtggtggcc
     8401 cacccgctct acccaagctt ccgacggaat cagacccggt tcttcgcccg gaaggtcgat
     8461 ccccgtgcgg cggcccaggc cgaggccggt ggcgtaatgg tcgatgcggt caattccgag
     8521 aattttgccg acattgtaga aaaagacgtc gcaggaagcc acgatgcctt tatgaagatc
     8581 cacgcttccg tgcgggtgat cgcaatggaa cacgcggccg tagaaagtgg cgtaacccgg
     8641 gcagtacacc gtgtaattcg cgggcagagc cttcgattcg agcatagccg tcgccatcac
     8701 gaccttgaaa accgagcccg gagcaagctg cgcctgaatc gcacgattga taagcggtgt
     8761 tttcgggtcg gtattaagct gagcccattc cttgttagga attcgcacgg agaaatcgtt
     8821 gggatcaaac gtgggccggc tgaccatcgc cagcacttcg cccgtgtgcg catccatggc
     8881 gacgagggcg ccttccttat tcgcgaaatc cgtttcggcg atctgctgca gatcttcatc
     8941 gatcgttaac tggatcggtt ttccgggaat cgcgtccacg ttgtcgagcg ttcgcattac
     9001 tttgccgacg ctattcacca ccacgcggcg catgccgtcc gttcccacga tctgatcgtt
     9061 gtattccttt tcgaggcctg cttttccaac gatgtctccc ggccggtagc gatcgggatt
     9121 tttctccaga tcttgcggtg aaacttcgcc cacgtaccca atcgtattgg cgagcatttc
     9181 gccgtgcgga tagcggcgtc gctgcaccat catcaaatca agcacgggaa gatcggcgcg
     9241 atgcgattcc acgaaagcga tgtccccttc ggtggccgcg ggcttgatga tcaccggcaa
     9301 aaacttcggt tcgttctttg cagcgtccaa ttgctgccgc agatcttcct tcgagattcc
     9361 cagtccatcc tcaatttgcg gcaacgcttt ctcgatgagt ttcggattgt cgcgaagtag
     9421 cagaatgctg aacgaaggat agctatccac gagcacgcgg ccatcgcgat cgagcatagc
     9481 tccgcgcggc gcgatgatgg cgatcgagcg tattccattg cggtccgcca aatcggaata
     9541 gtgcgaggat tggaccactt gaagcttcca aaagcccacc aaaagaaata tgacgaaggt
     9601 caccgaaagg taggaaaata acgccaggcg gccttgcgga atcttgcgat cgttaacgtt
     9661 ccatcctgcc aaacgagact cctgcgcagc ggggcgtcac gggaacccgg tgcttgatgc
     9721 ggtttttgag tccggccgaa gcgagccaag gtcgggactg tcaaaactat cattgcgaaa
     9781 cggtgcccgc aagcgcccgc gctcttaaat gtccttttgg tcaaccggcg tacaatatgc
     9841 gaaagcgaca aggcgaggcg cgcgcgtgtc ccggccgggg attttccgtc gcccagcatc
     9901 tttacacgcg ctcggcttgc gctccgcgct aaaatagtct tggatagatg cctgaaggtg
     9961 ccatgcaaat caacatttct ggctggcgaa aagcaaccct gttgtgtttg gcctgcgcgc
    10021 ttttcctgct gatcgcgccc atggcccatg cgcagcagac cgcgcgggca aaagccctcg
    10081 gcgcgcaatt gaagtgcgtg tgcggctgca accaggttct caccgcctgc aaccacgtgg
    10141 gctgcaccta ctcgcacgcc atgttgaaag agctggacga gagagttact cgcggagatt
    10201 ccgacagtct gatcctgcaa tccttcgtcc agcaatacgg agaagtggtt ctggcggagc
    10261 ccccgcggaa gggcttcaac tggctggtgt ggatcctgcc gatcgctgcg ccgctgctgg
    10321 cattctttat gatctgggaa gttgtgcggc gctggcgcca ccgctccgtt ctcgcgccgg
    10381 ccgagggtcc cggtctttcg tctgacttgc ttgatcgcgc ccgccgcgaa agcggggaga
    10441 acgaaattga gtaatctcga tatcgccgtc gcctgcattc ttctcgctct tgcgctcatg
    10501 ttttttgttt tcggcgttcg ctgggaacct tcggattcgg ctcctcatcg aaccaagctc
    10561 gatcagttga tggagcgacg cgacgcgatt tacgaaaatc tccgcgacct tcgcttcgag
    10621 cgtcgcgccg gcaaattttc ggagaaggat tacgatgaaa tcaagtcctc gctcgaaatc
    10681 gaagccgcgc gcgttcttgc cgacatggat actctgaccg gcggtgcgcc gacgcccgcc
    10741 acgcgccaag ccgcatccgc caaaggcccg cgctcatgat tgacatggca cgttgcgtga
    10801 cgaaatggct cgcgctggca tcgctatttg tttttctcgc ttcgcccgcc tttgcgggaa
    10861 ccgtcaccgg caccgtcacc aatggaacca cgggcaagcc cgcggccggc gtggaaatga
    10921 ttctgatcca gcttcagggc accatgaagc cgattgcgaa tacgaagacc gattccaacg
    10981 gccactacag tttcgataat cccgcgctcg gcgccggacc catgctcatt cgcgcggtgt
    11041 atcgcggcgt gaattatcac gagccggcca cgcccggcaa aaccaccgtt gatgtgcaag
    11101 tattcgagcc aaccgataaa gccagcgcgt tttcggtgac ggcgcacgcc atcatcctgc
    11161 agcccagcgg ctccactctg gtcgtcggcg aggaatacaa catttcgaac aagactcagc
    11221 ctcccgtcgc ctactaccgc cccaacggtt cgttttcatt cgcgctcccg gatggcgcgc
    11281 agttaaccga cgtctctgtt gtcggcacct ccggcatgcc agtggtacag actcctgtgg
    11341 acgaaggaaa cagcaagcag gccatcgact ttccatttcg cccgggcgat agcgtggtgc
    11401 gcatttcgta ccatctgcct tatccgtcca atcaggcaaa tcttcgcttc gtgtccgact
    11461 attcggccga ccgcctggcg gtttttgcgc cgccctcggt gcatttcacg ggcgccggtt
    11521 tcactcccgg cggagatgct cagggattta gcgtttacat gcgcgaatcc gtggccgcga
    11581 aaactcccat ttccgtaagc gtctccggca cagctccgcc gcccgcgccc gataatggcg
    11641 gctccagcaa tgcggccggc gctgacgact cgcagaatcc ttcggtgaat tcacacgccg
    11701 attcgggaca ggaaactccg gttgccaaca tcaccacatt tcccgcgcgt ctcgacagtt
    11761 tgaaatggat tctcgtcggc ggctttgctg ctctctttgt ggtcggcttg atttacgtgt
    11821 tgcgtcaacc ggaagccgcg gtaattggaa ctccgaacga aatggcagcg ccgcctttac
    11881 ccgatttggc cgcgcctccg ccgcaggcgg caactgcgaa acctcccgcg aatgtcgacc
    11941 aggaagttcg cagcagcctc gatcaattga aggacacgct gtttcgcctt gagttgcgcc
    12001 gcgaagccgg caccatcgcc gaagacgatt acatccgcga gcgcgaccgc gtccagaaaa
    12061 cgctgcgcga tttggtgaaa ggctgaatca tttgtccggc gctgtttctg actccgtggc
    12121 tccgagttcc gcccccgcga tttccgccgc tgctgccctg ggagtttcct tcgagcgcgt
    12181 cgaaaagcgt ttcggttctt tttttgcgct tcgcggcgtg tcgctcgaaa ttcaaaccgg
    12241 tgaatttgtc gcgctgcttg gtcccaacgg cgcgggaaag accacgctgc tgcgcctcgc
    12301 cgcgcttctc gccacgccca ccagcggccg cgtgcgtttc cccggcatcg cacccgaaaa
    12361 ttcaccgaaa tctctgatcg gcatggtagg ccacagcacg ctgctctacg acgaactgac
    12421 cgccaaggaa aatttggatt tctttgcgca actctacggc atcggcgatg ctggcgcgcg
    12481 tgtggctgca tccctcgaat cctgcgggct cgcattgcga gccaccagcc tggtccgcac
    12541 tttttcgcgc ggcatgcgcc agcggcttgc catcgcgcgc gcgctgttgc accgtcctcg
    12601 catgctgctg ctcgacgagc ctgccgccgg cttggatcgt caaggcctcg catggctcgc
    12661 ctcgacgctc gcgcggcttc gcgatagcgg cgccacggtg gtgatgagca cgcatgcgcg
    12721 caatgaatcg ctcgatctcg ccacgcgcgc cgt
//
LOCUS       DATNDT010000396         6507 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2125763, whole genome shotgun sequence.
ACCESSION   DATNDT010000396 DATNDT010000000
VERSION     DATNDT010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2125763"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..1049)
                     /locus_tag="VN788_12905"
     CDS             complement(111..1049)
                     /locus_tag="VN788_12905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21474.1"
                     /translation="MGSPKSSPSNRRVTRPERRPEVRHSLLPPVPHLRLREPFSNWPK
                     KVWDPSRYSLVNAPTSFTIHIRASTPPLTDPKLTEVPLVSAGPYLYWYEQALKLRLGT
                     PTPPPPPPPPSNPQPTPPPTATRHVYLLLQPTQQYVWHAPMFTIFSPTYVPPETDFVL
                     PIGVNVQFHDDDKSGWEFTGVLQASVNAYLWDHSRHDPGDVPQAQHGYTLSAQAQMAY
                     VLRLNDKFQTVFGVLAQPGVAFQWDPDSQGYRPNSALAVAGVAGLNYSFNDRLALGGQ
                     IQAGPTYGSPVTFDTTLAVNLTWNFVDFPRMIKLSR"
     gene            complement(1068..3140)
                     /gene="tssI"
                     /locus_tag="VN788_12910"
     CDS             complement(1068..3140)
                     /gene="tssI"
                     /locus_tag="VN788_12910"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HXU21475.1"
                     /translation="MVPFSQANRVLQITTPLGDNFVLLRGFSGFEAVSRLFEFHLDLV
                     APKPNVVNFPDILGKSVTVTINPPGLEARYFNGIITQIAKGANDGNFTYYRAVLSPIF
                     WCFTRVVQSRIFEQMSVPDILRQILVGFDTTYSLSGTYNARNYCVQYRESDFNFASRL
                     MEECGIRYYFVHSDGNHKMALTDTTWPTLSPEDSIALDDMGGGTRADDRIQSWELIQD
                     LASGKFTLWDYNFQLQGRNLQAAQTISSDPISLPTGDTINFQVGGNSKFEIYDFPGGY
                     AKRYDGIDAGGGEQAAELQKIFSDNTAVATLRMQAEEGRSLGIVGTSNARSLAPGNKF
                     TLLDGNNELGNYLLTSVQHTASQQVDYNTSGAGTFSYSNRFTCTPSQVPYLPIDPSVR
                     MPVIGLQTAFVTGPAGQDNEPFVDKYGRVHVQFNWDRLGQKDTNSSCWLRVVQPWAGK
                     TWGHQWFPRIGHEVVVDFLEGDPDNPIIIGSVYNPTCMPTYELPANSFMSGIKTRSTP
                     GGGDSNFNELRFEDKTGKELIAVHAEKDLQTVVENDEAHQIGANQYITIQTNRNLTVG
                     GNVNAKIVGNDNYTLQGNSNAKITGTLALQVTGSATENFQASHTTNTTSSETIIGESI
                     LLQATNSITIVAGASAIVLGPSGITIDGAMVKINMPTPPPPPTVVTPGSPPDEPETAP
                     QMGTPPNS"
     gene            3339..4700
                     /gene="tssK"
                     /locus_tag="VN788_12915"
     CDS             3339..4700
                     /gene="tssK"
                     /locus_tag="VN788_12915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HXU21476.1"
                     /translation="MKNSHRVVWTKGMFLAPQHFQAQDAFFESQLQFRATASSALNWG
                     LTDMVVDQESLANGIFTIRHCRGILQDGLAFSIPDSDPAPEGRPIAEFFPPTQLDLDV
                     FLTIPEQRERGKNFTAVQDPSEIATASTRYVSETSSVLDEAGGTEEKPLQLGRRNFRI
                     GFGGENLEGSSYIRIAQITRNPSGAYVMKQEFIPPSLSITSSEYLMMLLRREVELLTA
                     KSSSLSMLRRQKGRSLAEFGSADVANFWLLHTANTYLPLLKHFWTMRRVHPEQLFLTM
                     LALAGALSTFSLDEHARNLPDYNHEELGVSFTLLDLKIRELLETAIPSKFVLVPLSLT
                     EKSIWTGIVPKEEYFKKTQFFLAVGAQMGIDDIIKQTPKLIKVSPPVEIHRLIRNALP
                     GLGLRHAPVPPPAIPAKLDRQYFSLNQSGILWDGIVKGSQVSIFTPDEIVKPELELLI
                     VLE"
     gene            complement(4701..5885)
                     /locus_tag="VN788_12920"
     CDS             complement(4701..5885)
                     /locus_tag="VN788_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21477.1"
                     /translation="MKAVIALLFVFAGICSAQIAIRGEKVYTMAGPPIENGIVLIENG
                     KISVVGPASSVTIPAGTKTLRAKVVTPGLVDAHSTVGLTGYLNQAQDQDELEKSAAIQ
                     PELRAIDAYDFDERLIEWVRGFGVTTMNTGHASGELISGQTMIVKTRGASAEEAAIVP
                     VAMVACSLGDEGLAGEGKSPGTRAKEVAMLREEFIKAREYANKKKDAPRDLRLEVLAR
                     VLKGELPLLVTAQRSRDIMDALRLGKEFKLKIILDGAAEAPLVLNEIKASGYPVIVHP
                     TMYRALGETENLSFETAAKLQAAGIPWALQSGYESYVPKTRVILFEAAIAAANGLTFD
                     QALAGITINAARIIGVANRAGSLETGKDGDVALYDGDPFEYTSHCVGTVIEGRQVSAE
                     VR"
     gene            complement(5882..>6507)
                     /locus_tag="VN788_12925"
     CDS             complement(5882..>6507)
                     /locus_tag="VN788_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011403883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21478.1"
                     /translation="VEVLDGKRTVQFHTHRHDDILTVIRLSQEFHFHVVLHHVSDGWM
                     VADEIAKAGVPCSVILIDSPGGKEEAKNVSFKTAAVLDKAGVLVGFHTDDPITDSRLF
                     LRSAALAARAGLSREKALYGVTMANARIIGLQDRIGTLEPGKDADFILLSGDPLSVYT
                     HVLQTWIEGKKVFDRSDPKDYLYAVGGYGAGHDQPPTLDWDGEDEVQ"
BASE COUNT         1318 a         1853 c         1959 g         1377 t
ORIGIN      
        1 cgtcggcgag gacggcaact tcacctacgc ttacgcgtga ggagagaagg cggactggct
       61 gagaaaaggc cagtccgccc gcctcggaat catcgcctgg agatccggca ttatcgcgag
      121 agcttgatca ttcgcgggaa gtccacgaag ttccaggtca ggttgaccgc cagagtggtg
      181 tcgaaggtca ccgggctgcc gtacgtaggt ccggcctgga tctgccctcc caacgccaac
      241 cggtcgttaa acgagtaatt caggccggcg actccggcca cggccagcgc cgagttcggg
      301 cggtagcctt gcgaatccgg gtcccactga aaggccacgc ccggctgtgc caggactccg
      361 aaaacggtct ggaacttgtc gttcaggcgg aggacatagg ccatttgcgc ttgagcggac
      421 agcgtgtaac catgctgcgc ctggggaacg tcgccgggat cgtggcggct gtggtcccaa
      481 aggtacgcgt tcacgctcgc ttgcaggacg cccgtaaatt cccaaccgga cttgtcatcg
      541 tcgtgaaact gtacgttcac tccgatcggg agcacgaaat ccgtttccgg tggaacgtag
      601 gtaggagaaa aaatcgtgaa catcggagcg tgccagacat actgctgcgt cggctgcaac
      661 agcaggtaga catggcgggt tgccgtcggg ggcggtgtcg gctgtgggtt ggatgggggt
      721 ggaggaggcg gaggcggagt cggcgtgcca agccggagtt tgagcgcctg ctcgtaccaa
      781 tacaaataag ggcccgccga taccaatggc acctcggtca atttaggatc cgtcaacggc
      841 ggcgtggaag cgcggatgtg gatggtgaag gaagtgggcg cattcactag cgaatagcgc
      901 gacgggtccc aaaccttctt gggccaattc gaaaagggct cacgcaatcg cagatgcggg
      961 accggcggca gcagcgaatg gcggacctcg ggccggcgct ccggccgggt tacccggcga
     1021 ttcgacggac tgcttttcgg actgcccata gctttgcgct ccggcgatca ggaattgggg
     1081 ggcgtgccca tttgcggcgc ggtctcgggc tcgtcgggcg gacttccggg cgtgacgacc
     1141 gtgggcggcg gaggcggagt gggcatattg atcttgacca tggcgccgtc gatggtgatt
     1201 ccgctcgggc cgagcacgat ggcgctggcg cccgcgacaa tcgtgatgga attggtcgcc
     1261 tgcagcaaaa tactctcgcc gatgatcgtt tccgaactgg tcgtattggt ggtgtggctg
     1321 gcttgaaagt tctcagtggc cgagcccgtc acctggagcg ccagcgtgcc cgtgatcttg
     1381 gcgttgctgt tgccttgcag agtatagttg tcgttcccga cgatctttgc gttgacattg
     1441 ccgccgacgg tgaggttgcg gttggtctgg atggtgatgt actggttcgc tccgatctgg
     1501 tgcgcttcat cattttcgac gaccgtctga agatcctttt cggcatgaac ggcaatcagt
     1561 tctttcccgg ttttgtcctc aaagcgcagc tcgttgaagt tgctgtcgcc gcctccgggg
     1621 gtgctgcgcg ttttgatgcc gctcatgaag gagttcgccg gcaactcata agtcggcatg
     1681 caagtcgggt tgtaaaccga gccgatgata atcggattgt ccggatcgcc ctcgaggaaa
     1741 tccacgacga cttcgtggcc gatgcgcgga aaccactgat gcccccaggt cttcccggcc
     1801 cacggctgca ccacgcgcag ccagcatgag ctgttggtat ccttctggcc cagacggtcc
     1861 caattgaatt gcacgtggac gcggccgtat ttatcgacaa acggctcatt atcctggccg
     1921 gctggcccag tcacaaaagc ggtttgcagg ccaatcaccg gcatgcggac cgacggatcg
     1981 atcggcagat aaggcacctg cgaaggcgtg caagtaaaac ggttcgagta cgagaacgtc
     2041 cccgcgccgc tggtgttgta atcgacctgc tgggaggcag tgtgctgcac ggacgttagc
     2101 aggtagtttc ccagttcgtt gtttccatcg agaagcgtga atttattgcc cggcgcgagc
     2161 gagcgggcgt tactcgtgcc tacgattccc aggctgcggc cttcttcggc ctgcatgcga
     2221 agggtggcca ctgcggtatt gtccgaaaag atcttttgca gctcggcggc ctgctcgccg
     2281 ccgcccgcat cgatgccgtc atatcgtttt gcatacccgc cgggaaaatc gtagatctcg
     2341 aacttggaat tgccgccaac ctggaaattg atcgtgtcgc cggttgggag cgaaatcgga
     2401 tcggacgaga tggtctgcgc ggcttgaaga ttcctgcctt gcaattgaaa attgtaatcc
     2461 cacagtgtaa atttaccgga ggcgagatcc tgaatcagtt cccaggattg aatccggtcg
     2521 tcggcgcggg ttccgccgcc catgtcgtcg agagcgatgg agtcttccgg cgaaagcgtc
     2581 ggccacgtgg tatcggtcag cgccatcttg tggttgccgt cggaatgcac gaaatagtaa
     2641 cggatgccgc attcttccat caagcggctg gcaaaattga agtcgctttc ccggtattga
     2701 acgcaataat tgcgcgcatt gtacgtcccg ctgaggctat aagtcgtatc gaaccccacc
     2761 aggatctgcc ggagaatgtc ggggacggac atctgctcga aaatccggga ttggacaacg
     2821 cgcgtaaagc accagaagat aggactgaga accgcccggt aataggtgaa gttgccgtcg
     2881 ttcgcgccct tggcgatctg ggtgataatg ccgttgaaat accgggcctc caggcctgga
     2941 gggttgatcg taacggtcac gctcttgccg agaatgtcgg ggaagttgac cacgttgggt
     3001 ttgggcgcga cgagatcgag atggaactca aacagccgcg atacggcctc gaatccggag
     3061 aagccccgta aaaggacaaa attatcacca agaggagtgg tgatctggag cactcgatta
     3121 gcttgagaaa atgggaccac aattcgcctc cggaatcagg ttatggtgcg gaaaactcgt
     3181 cttaagacac tccatcatac cgagaaccaa aaagattgtg tcggaaacca cgcaacgcgt
     3241 atcgctttgg ccgcagataa ccggtggagc ggccagggcc ggatgatttt ccggcgtgcc
     3301 gcactatact ggaagattcg cgctacgaaa gtctctctat gaagaattcg catcgagtgg
     3361 tctggacgaa gggcatgttc ctggcgcccc agcacttcca ggcgcaggac gcattttttg
     3421 aaagccagct ccaattccgg gctaccgcat cgtcggccct caactgggga ttaaccgaca
     3481 tggtagtgga tcaggaaagc cttgctaacg gcatcttcac gattcgccac tgccggggga
     3541 tcctccagga tggtctcgcc ttcagtattc ccgattcgga tccggcgccg gagggtcggc
     3601 cgatcgccga gtttttcccg cccacgcagc ttgatctgga cgtgttcctg accatccccg
     3661 agcagcgtga gcgcggcaag aacttcaccg cggtgcagga cccgtcggaa atcgcgacag
     3721 cgtccacgcg ctatgtaagc gaaacaagtt cggttttgga cgaagcaggc ggtaccgagg
     3781 aaaagccgct ccaactcgga cgaagaaatt tccggatcgg atttggcggg gagaatcttg
     3841 agggctcgtc gtacatccgg atcgcgcaga ttacccggaa tccgtccggc gcgtacgtga
     3901 tgaagcagga attcataccg ccgagcctta gcatcacgtc gagcgaatac ctgatgatgc
     3961 tgttgcgaag agaggtggag ttgctgaccg cgaaaagcag ttcgctttca atgttaaggc
     4021 gtcagaaggg gcgaagcctc gccgagttcg gctcggcgga cgtcgcgaat ttctggttgc
     4081 tgcataccgc gaacacgtat ctgccgctgt taaaacattt ctggacgatg cggagagtcc
     4141 atcccgagca gcttttcctc acgatgcttg cattggctgg cgccttatcg actttctcgc
     4201 tcgacgagca cgcgcgcaat ctgcccgact acaaccatga ggaactgggc gtctctttta
     4261 cgctgctcga tctgaagatt cgtgagctgc tggagacggc gattccgtcg aagttcgtgc
     4321 tcgtgccttt atcgctgacg gagaaatcga tctggacggg aatcgtgccg aaagaggagt
     4381 acttcaagaa gacgcaattt ttcctggcgg tgggcgctca gatgggcatc gacgacatca
     4441 tcaaacaaac gcccaagctg atcaaggttt caccgcccgt ggaaattcac agattgatcc
     4501 gcaatgcgct gccgggcctc ggattacggc acgcgcccgt accgccgccc gccataccgg
     4561 cgaagctcga ccgccagtac ttttcgctca atcagagcgg aatactctgg gacggcatcg
     4621 tgaaaggatc gcaggtcagc atcttcacac ccgacgagat cgtgaagccg gaactggaat
     4681 tactgatcgt gctggagtag ttaccgcact tccgcgctga cctgccgacc ctcgattacc
     4741 gttcccacgc agtgggacgt gtattcgaat gggtcgccgt cgtacagcgc aacgtctcca
     4801 tccttgcctg tttcgagcga tccggcgcga tttgcgacgc cgatgatacg cgccgcgttg
     4861 atggtgatgc ccgccagcgc ctgatcgaaa gtaaggccat tcgcggcggc gatagcggct
     4921 tcaaacagga tgactcgcgt tttcgggaca taactctcgt agccgctttg caacgcccat
     4981 ggaattccgg cggcttgcag ttttgccgcg gtttcgaagc tgagattctc ggtctcaccg
     5041 agcgcccgat acatcgtcgg atggacgatc accgggtatc ccgaagcctt gatttcattg
     5101 agcacgagag gcgcttcggc cgcgccgtcc agaatgatct tcagtttgaa ttccttgccc
     5161 aacctgagcg cgtccatgat gtcgcgcgag cgctgcgccg tcaccagcaa cggaagctcg
     5221 cctttcaaca cgcgcgccag cacttccagg cgcaaatcgc gcggcgcgtc cttcttcttg
     5281 ttcgcgtatt cccgtgcttt gatgaactct tcacgcagca tcgcgacttc cttcgcgcgc
     5341 gtgcctggac tcttgccctc tccggcgagg ccctcatctc cgagcgaaca cgcgaccatg
     5401 gccaccggga cgattgccgc ctcctcggcc gaagcgcccc gtgtcttcac gatcatcgtc
     5461 tgaccggaaa tgagttcgcc cgacgcgtgg ccggtgttca tcgtggtgac accgaagccc
     5521 cgtacccact cgatcaggcg ttcgtcgaaa tcgtaagcat cgatggcgcg aagctccggc
     5581 tgaatggccg cgctcttttc gagttcgtcc tgatcctgtg cttgattcag atatccggtg
     5641 agtccgacgg tggaatgcgc atccactagg ccgggtgtta ctactttcgc gcgcagcgtc
     5701 ttcgtgcctg cagggattgt caccgatgag gccggcccca cgacgctgat cttgccgttt
     5761 tcgatgagaa cgatgccatt ctcaatcggc ggccccgcca tcgtgtagac tttttcgccg
     5821 cggatcgcaa tctgcgcgga acagattccg gcgaacacga acaataaagc gataacagcc
     5881 ttcattgaac ttcatcctcg ccgtcccagt cgagtgtcgg cggctgatcg tgacccgcgc
     5941 cgtagccgcc gacagcgtat agatagtctt ttggatcgct gcggtcgaac actttctttc
     6001 cttcgatcca tgtctggagg acgtgcgtgt aaacactcag agggtcgcca gaaagcaaaa
     6061 tgaaatcggc gtctttgccg ggttcgagag ttccgattcg atcctgtaat ccgatgattc
     6121 gcgcattcgc catcgtcacg ccgtagagag ccttttcacg ggaaagtcca gcgcgcgcag
     6181 ccagggccgc gctgcgtagg aagagtcgcg aatcggtaat cgggtcatcg gtgtgaaatc
     6241 cgactagcac tccggcttta tcgagcaccg ccgccgtctt gaagctcaca ttcttcgctt
     6301 cctccttgcc accagggctg tcgatgagga tgacagagca gggaacgccc gccttcgcga
     6361 tctcatcggc aaccatccaa ccgtcgctga cgtgatgcag aacgacatgg aagtgaaact
     6421 cctgcgacag gcggatcacc gtgagaatat cgtcatgccg atgcgtgtgg aactgcacgg
     6481 tgcgcttgcc gtccagcact tcgacca
//
LOCUS       DATNDT010000397         4342 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2966532, whole genome shotgun sequence.
ACCESSION   DATNDT010000397 DATNDT010000000
VERSION     DATNDT010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2966532"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VN788_12930"
     CDS             <1..319
                     /locus_tag="VN788_12930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21479.1"
                     /translation="FDIPSKVTDYVYHCRFSHCWNAIATRHSDTMDCKFIVDGKGVIL
                     GLAHPGFVTFRERAGRNLSDREASYIAAEYLRGRLEQEDEHSLYDVSAADVLRIIEKL
                     AIH"
     gene            387..1223
                     /locus_tag="VN788_12935"
     CDS             387..1223
                     /locus_tag="VN788_12935"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HXU21480.1"
                     /translation="MLVLPSDQMNHLLSLIVHHGYLVMFLLVLAEAVGVPVPAALALI
                     AGGAAVASGALRGPALAALAIAAMLLGDTLFYLLGNFMGWNLLGFLCRVSVNPETCIL
                     RAAESFYKRGRAALVVAKFIPGVNTMAPPLAGSMEMPFVQFLGLDLLGAAAYTFAYGA
                     LGYFFRDFIASITRGVQAAGHAVELVVVLALIAFIAYRFSLYWKHRADRVVPRVQVAE
                     LARKLQSAGPGKILLVDVRSHGYYDPGAARIQGSIRIEPNNLADEIKTLPKEKDIYLY
                     CT"
     gene            1551..2330
                     /locus_tag="VN788_12940"
     CDS             1551..2330
                     /locus_tag="VN788_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21481.1"
                     /translation="MRKLIFLTALILGIFLRGTPAAADSSAESSASPVIVIGFVGGFV
                     HGDNMIHSEVQLAAKLRSMYPDGVYAESLANHRAKSAYPEIVRLLDANRDGALTSAEK
                     RSARIILYGHSWGASQALTLARKLQKSGIPVLLTIQVDSVKKRHQNDALIPANVAEAA
                     NFYQPHGLLHGRARIRAADPERTKILGNFRFDYSKTPARCDGNYPWWDRFIAKAHIEI
                     ECDPTVWSRVESLILHKLPPAASSASMSPAGDASPAAQSRQ"
     gene            complement(2603..>4342)
                     /locus_tag="VN788_12945"
     CDS             complement(2603..>4342)
                     /locus_tag="VN788_12945"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU21482.1"
                     /translation="GKTGDQWLMAAGNYASTRYSPLTQIDSSNVSRLGLAWYYDTGSL
                     PGTLEASPVISNGTLYGTVTWGAVFAVDARTGKEKWTWDPHEGHHNFPPGSEGNPNKV
                     RTGPSLCCGPGNRGVAVYDGRVYVGTLDARLVALDAETGKVDWDVPTAEKNTDYSITG
                     APLIVKGKVIIGNGGGEFAVRGYISAYDAETGKLDWRFYTVPGDPSKPFENKAMEVAA
                     KTWSKDVPWWKLGGGGNPWNAISYDPELDLLYFGTGQGGPWVQKYRSEMGMDNLYICS
                     IIAVKPETGKFVWYFQTTPGDEWDYDAIADLVLADIKIDGKVRHVIMQAPKNGYFYVI
                     DRKTGKFISGAALQKITWAKGLDPKTGRPIVNPEAHYGTEGTVIWPGPGGGHVWQGMS
                     YNPQTQLVYVTPGMASSFNYVQAKEFKPELGEYNWGIVFRDAAGRGTGPRAGGANGAP
                     PPPPQPRTPPAGNFLVAWDPATETARWRVPATGGGGTMTTAGNLVFAESNAGDFEAFS
                     ADKGEKLWSVKLVPGFANPVTYMLDGRQYVSVLAGRSKGRLYTFALDGNVPVPPPPTA
                     PSTGATPPAGEAR"
BASE COUNT          807 a         1451 c         1152 g          932 t
ORIGIN      
        1 cttcgatatc ccgtctaaag taaccgatta cgtttaccac tgccgtttct cccattgctg
       61 gaatgccatc gccacgcgcc acagcgacac catggattgc aaattcatcg ttgatggaaa
      121 aggagtgatt ctaggcctcg cacatccagg tttcgtcact tttcgcgagc gggccggccg
      181 caacttgagc gatcgcgaag caagttacat cgccgccgaa tatttgcgcg gccgcctcga
      241 gcaggaagac gaacattccc tctacgacgt ttctgccgcg gatgtcctcc gcattatcga
      301 aaaactagcc atccactagc ctaacctgcg tttgccgaac gcattcgcac gggcaaaacg
      361 cacgcattcg ccgcgcggca agttgcatgc tagtcttgcc ttctgaccag atgaaccacc
      421 tcctgtcgct gatcgtccat cacggatatc tcgtcatgtt cctgctcgtt cttgcggaag
      481 cagtcggggt accggtgccg gctgctctgg cgctgatcgc cggcggggct gcggtggcgt
      541 cgggcgctct gcgcggaccc gccctggcgg cactcgctat cgcggcgatg ctcctcggcg
      601 acacgctgtt ctaccttctc ggcaacttta tgggctggaa tttattgggt tttctctgca
      661 gggtttctgt caacccggaa acatgcatcc tgcgcgccgc ggaatcattc tacaaacgtg
      721 gccgcgccgc cctggtggta gccaaattca ttcccggcgt caacaccatg gctccgccgc
      781 tagcgggcag catggaaatg cctttcgtgc agttcctcgg cctcgatttg ctgggcgccg
      841 ctgcgtatac attcgcatat ggcgctctcg gatatttttt ccgcgatttc attgcgagca
      901 tcacgcgcgg cgttcaagca gccggacatg ccgtcgaact cgtcgtcgta ctcgccctga
      961 tcgctttcat tgcgtaccgc ttctcgctct actggaagca tcgcgcggac cgcgttgtgc
     1021 ccagagtcca agtcgccgaa ctcgcgcgca agctgcagtc ggcaggcccg ggcaagatcc
     1081 tgctggtcga cgtgcgcagc cacggatact acgatcccgg cgccgcacga atccaaggct
     1141 cgattcgcat cgagcccaat aacctcgccg acgaaattaa aactcttccg aaggaaaaag
     1201 acatctacct ctattgcact tgacgccgcg aagccaccag cgcccgtgtg gcgcatctcc
     1261 tgcgcgacca gggattcaac gcgttcgtca tcgtcggcgg cctcgcggcc tggcgcaaag
     1321 ccggcgaacc agttgagccc gtcccgcaat ccgacctggt gcacctccca actttcagcc
     1381 gccctcgtgc ccgctgacca tctgtctgca ttcgtagcgg cacggcatgc aacgccccgc
     1441 aaatatgcgt ggctcctcgc tcgctttcgg cgcaaatcca cgggggctga tgttgcgttt
     1501 gcgatcgaaa tcgtttcttg gtaggattcc gcgcgtcccc gcccatgtaa atgcgcaaac
     1561 tcattttctt gacggccttg atcttgggca tatttctccg cggtacgccc gccgctgcgg
     1621 attcctccgc tgaatcctcc gcttcgcccg tgatcgtcat cggcttcgtc ggcggattcg
     1681 tccacggcga caacatgatt cacagcgaag tccagttggc cgccaaactt cgctccatgt
     1741 atccagacgg cgtctacgcc gaatcactcg cgaaccatcg cgcgaagagc gcctacccgg
     1801 aaatcgttcg cctgcttgac gccaatcgcg atggtgcgct cacctccgcc gaaaaacgca
     1861 gcgcgcgcat catcctttac ggacatagct ggggcgcgtc ccaagcgctc actctcgcgc
     1921 gaaaattgca aaagagcggc atccccgtgc ttctcaccat tcaggtggat agcgtcaaaa
     1981 aacgccatca aaatgacgcg ctcatccccg ccaacgtcgc cgaagccgcc aatttctacc
     2041 agccgcatgg ccttctgcac ggccgcgcgc gtattcgcgc cgccgatccg gaacgcacca
     2101 aaattctcgg aaattttcgc ttcgattaca gcaagacgcc ggcccgctgc gatggcaatt
     2161 atccctggtg ggaccgcttc atcgccaaag cgcacatcga aatcgaatgc gatcccaccg
     2221 tctggagccg cgtggaaagc ctcatcctcc ataagctacc gcccgccgct tcgagcgcga
     2281 gcatgtcgcc tgctggggac gcatcgcctg cggctcaatc ccgtcaatag ctccctacgc
     2341 cgatatctga ctacagcgct tttcggccgg taaccggtat ttgcctgatt tcccgcaggg
     2401 cacgcggcgc gctacatttg tcgcgtggag gacgactcct atgcgcttgc tcaagtacct
     2461 ggcgccactg gctctcagcg cgtgcgttgt ggtgccggcg atgatcgaag gcctgcgcga
     2521 ctcgcgtcga aaccggccgc ggctacggcg actgtgcgat cacgacaact agttgcttgc
     2581 ctgacttctc gcccggatgc tcctaccggg cttcgccagc gggcggagtt gcaccggtac
     2641 taggcgcggt gggcggcgga ggcaccggaa cgtttccgtc gagtgcgaaa gtatataggc
     2701 ggcccttgct gcgtcccgcg agaacggaaa cgtactgcct cccatcgagc atgtaggtaa
     2761 ccggattcgc aaatcccggc accagcttca cgctccagag tttttcgcct ttgtccgcgc
     2821 tgaacgcttc gaaatctccc gcattggatt cggcgaatac taaattcccg gccgtggtca
     2881 tcgtgccgcc accacccgtt gccggaacgc gccagcgcgc cgtttccgtc gccggatccc
     2941 acgcgacaag gaaatttccc gccggcggcg ttcgaggttg gggcggcgga ggcggcgcgc
     3001 cgttggcacc ccctgcgcgc ggacccgtgc ctctgcctgc ggcatctctg aaaacaattc
     3061 cccagttgta ctcaccgagc tcgggcttga attctttcgc ctgaacataa ttgaagctcg
     3121 aagccattcc gggcgtgacg taaacgagct gcgtctgcgg gttgtaggac attccctgcc
     3181 aaacgtgtcc gccaccaggt cccggccaga tgactgtgcc ctcggtaccg taatgcgctt
     3241 cgggattcac tatcgggcgg cccgtcttcg gatcgagtcc ctttgcccag gtaattttct
     3301 gcagcgccgc gccggagata aatttccccg tcttgcgatc gatcacgtag aagtaaccgt
     3361 tcttcggcgc ctgcatgatt acgtgccgca cttttccatc gatcttgata tctgcgagga
     3421 ccaaatccgc gatggcatcg tagtcccact catcgccggg cgtcgtctgg aaataccaga
     3481 cgaattttcc cgtttccggc ttcacggcga tgatcgagca gatgtacaaa ttgtccatgc
     3541 ccatctcgct gcgatatttt tgaacccacg ggccgccctg tcccgttccg aagtagagca
     3601 gatcaagctc cgggtcgtag gaaatcgcgt tccagggatt gccgcctccg cccaatttcc
     3661 accacggaac atccttcgac caggttttcg cggcaacctc catcgcttta ttctcgaacg
     3721 gcttggacgg atcgcccggc accgtgtaga agcgccagtc gagctttcct gtttccgcgt
     3781 cgtacgccga aatgtagccc cgcaccgcaa actctccgcc gccgtttccg ataatcactt
     3841 tccctttaac gatcagcggc gcgcccgtga tgctgtagtc cgtgtttttc tcggcggtcg
     3901 ggacatccca atccactttg cccgtttccg catccagcgc gaccagcctc gcgtccagcg
     3961 tgccgacgta cactctcccg tcatacaccg cgacaccgcg atttcccggc ccgcagcaga
     4021 gactcggtcc ggtgcgaacc ttattaggat ttccctccga tccaggagga aaattgtgat
     4081 ggccctcgtg cggatcccac gtccactttt cttttcccgt gcgcgcgtcc accgcaaaca
     4141 ccgcgcccca cgtcaccgtt ccgtaaagag tgccattcga aatcaccgga gatgcttcga
     4201 gcgtgccggg cagagagcca gtgtcgtaat accacgcgag cccaagccgg ctcacattcg
     4261 aagaatcaat ctgcgtcagc ggactgtatc gcgtcgaagc ataattgccg gcggccatca
     4321 gccattggtc tcccgtcttg cc
//
LOCUS       DATNDT010000398         8323 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4211074, whole genome shotgun sequence.
ACCESSION   DATNDT010000398 DATNDT010000000
VERSION     DATNDT010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8323)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8323)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8323
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4211074"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VN788_12950"
     CDS             <1..485
                     /locus_tag="VN788_12950"
                     /inference="COORDINATES: protein motif:HMM:NF024682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXU21483.1"
                     /translation="VPFFDAAYREVEQRYPNGARKLKFNEALKRVLNRLVTDLIEHTQ
                     KEVDASGVRTAEEIRSSPKRLAGFSVEVARNSAGLKNFLNSNVYSLPVMAEERERSVA
                     ALDALFRFFVEQPARMPRHYAELARTTPVHRVVCDYIAGMTDHFLLRQCSDLCVNLRQ
                     "
     gene            731..1087
                     /locus_tag="VN788_12955"
     CDS             731..1087
                     /locus_tag="VN788_12955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU21484.1"
                     /translation="MPTNNNTAGVWLFIPDATESLMNSATLLGSIPFGKDSLDIFKIS
                     DGHFALAFVDADGDDSISSVGSVTSTAPESDPVATDDSTPTNTPEPSSLALLGLGTIA
                     LGLAFAGRRLKPTTAG"
     gene            1404..2504
                     /locus_tag="VN788_12960"
     CDS             1404..2504
                     /locus_tag="VN788_12960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21485.1"
                     /translation="MKLVRGVVFASALFVAFGMLARAQRGGKRTASAAAPRQSPQLFT
                     EPVLVKGSPTNLRFFDVSWNDNSKQRYYLADRTNNAIDLIDSATDTFLGFIGKGHYTG
                     SRPCPNNPKDMRLCAGPDGVVTDDLGHVWSGDGDANIIEADDKPGSTIIRSIPTGGKG
                     RIDELAYDPIDRILLAENDGDSPPYVIFVSVPGGTVLGRYVYPKSQDGMEQPAWVRET
                     DMFYQNVPGPKNRLDVFDPHHLPKPVRAFPVDCTGGLLGLTLSGLTVGPNARLMTVCG
                     SVGGVAIDSRTGRVGKPIPEGADADEVWYDPGAGNYYFSRNSNLAVVNASTEKFVTYV
                     PLGSHSVAANAKNKHVFVPVAGKGIFMVAPRQ"
     gene            2828..3760
                     /locus_tag="VN788_12965"
     CDS             2828..3760
                     /locus_tag="VN788_12965"
                     /inference="COORDINATES: protein motif:HMM:NF021357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HXU21486.1"
                     /translation="MAIARVVDGAAWLLLTFTLPMKSASQRVQVWRKLQRYGAIPLGN
                     SGYLLPNNPAAQERFEWLATAIRKYAGDASVVKVQSIDNLSTPQLVGRFAEARGRDYQ
                     ELIRELRKILSGRERKSTPGRLSRLRARFLEIAQVDFFNSPLQKRVEELLARADASPA
                     PTEETVKADPREYVGRAWITRPRPGIDRSASAWLIQRFIDKKARFIFGPEDRVPREAV
                     PYDMFHGGFGHRGEDCTFETLQKQFRIHDRKVRVIGEMIHDADLFDEKFGRREGFGID
                     EVLNGWAKQGIPDGELLKRGMQLIEALYHSLSGK"
     gene            3817..4686
                     /locus_tag="VN788_12970"
     CDS             3817..4686
                     /locus_tag="VN788_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018315790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HXU21487.1"
                     /translation="MRRLLMVISILVFPIVVSAQELDIAKIDQVLGRSGQKTGDVYKV
                     GFPRTDLHVVVHGVAIKPGFALGSWAAFSGSSANARVMGDLVLLQDEVNPVMEKLRSA
                     GFEITAVHNHLIDESPRVMYMHYMGHGPAAQLASSLRAALAVSKTPLGKPAATAEPSS
                     APAWVKTVEDAVGRKGSFKGGVLAFGIPRANAVTMGGMTIAPAEGVAESINFQDAGPG
                     KVATTGDFVMTAEEVNPVISTLEQHHISVMALHSHMLAEQPRLFFMHFWSVGSPGSVA
                     EGIKAALSHISTK"
     gene            4738..5550
                     /locus_tag="VN788_12975"
     CDS             4738..5550
                     /locus_tag="VN788_12975"
                     /inference="COORDINATES: protein motif:HMM:NF024946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HXU21488.1"
                     /translation="MRAEHLSSNWQVLGVVLLLLFCANRTTAQGGPPLLTNDPVTPDH
                     GNWEINLGAMPVLTKITKSVEVLQIDLNYALTDRIELIYEVPFVWQSATGQPNATGWG
                     NGLPGVKLRLIDGGENGWNLSTFPQVQIGGSAASVKKGLADEGTRFLLPFEVSKRFGP
                     LNADFEVGYFFPLDSSASRDERILGLALGHKLTPKLETIGEIYDDKVMGAPEHNTEFD
                     MGGRWEFHKGLLFLFMAGRSFKGNSSGQPEFLGYGGIRILLDHYGTRLHSDK"
     gene            5665..7311
                     /locus_tag="VN788_12980"
     CDS             5665..7311
                     /locus_tag="VN788_12980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014787128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU21489.1"
                     /translation="MKTWKAFVLGLIAAVVVAAIGGILFVHRGFRATNQPLPLEAVVA
                     RAARNWSIPGRARNDKNPLKLTSENLRQGREEFLARCASCHGHDGSGLTDMGRSLYPR
                     TPDLRSNETQNLSDGEIHYIIANGVQLTGMPAWSNPHQERNVNAWSLVLFVRSLRPLT
                     REEKAQQASTAKSAHYVGSQACEKCHTQIYDHWKKTPMANIVRDPREHPDAIIPNLAT
                     NNVYKFTKDQVAFVYGSIWKQRYFTKVGDDYFPLPVQWEVNNHKWSKYFAGPKADWWT
                     KFYPPDNMKRPTGPTCDGCHSVGYDIHTKKVAEWNVGCERCHGPGSEHLADPRRDNIV
                     NPAHMDYVAASDTCIQCHSQGRPLTIPIEGKYYDWPVGYQLGLRLQDFWKLEDHTLAE
                     TNFYYFADGTAHKNRMQGNDFMQSVMYRRGITCFDCHDVHGTNNSAQLRESADKLCLE
                     CHGPLSPNGPRTATLEEHTHHKEGSAGSQCVACHMPAIETEGPPNTMVHAHTFRFITP
                     AMTDKYKIPNPCTSCHKDKSTAWATNEMSKWPERSPWRLE"
     gene            7576..>8323
                     /locus_tag="VN788_12985"
     CDS             7576..>8323
                     /locus_tag="VN788_12985"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU21490.1"
                     /translation="MAPLRNGTVLEERRKYVRHRLTSTVYVELGPGNGGIIVNLGTGG
                     LSMHAAVKLNAAMELALRFRLDRTEEPIEVAGRVAWLGPTQKEAGIAFSDLPSSAEPR
                     IADWIAEQENPASDTQRDLEPQSKSSLTKSAAPPLPIRASAAESLVAQKSESVLPRSS
                     MGIPHLGTSESSQLHNFLSTTPDLPETPPAIAFRTTEERFEKPSDKLLRSPAKRYEVL
                     LEPEKPMPAAKEILPQETASPSLTPASSKVI"
BASE COUNT         1854 a         2236 c         2429 g         1804 t
ORIGIN      
        1 aagttccctt ttttgacgcg gcctaccgcg aagtggagca gcgctatccg aatggcgcgc
       61 gcaaattaaa gttcaatgaa gcgctgaagc gcgttctgaa tcgcctggtc accgatttaa
      121 tcgagcatac ccagaaggaa gtggatgcga gcggcgtgcg gaccgcggaa gaaattcgca
      181 gctctcctaa aaggctggcc ggtttttccg tggaggttgc gcgaaatagc gccggcctca
      241 agaattttct caactccaat gtctattcgc ttccggttat ggccgaagaa cgagaacgtt
      301 ccgtggccgc gcttgacgcg ctctttcgct ttttcgtcga gcaacccgcc agaatgccgc
      361 gccattacgc cgaattggcg cgaacaacgc cggtgcatcg agtcgtatgc gactacatcg
      421 ccgggatgac tgatcatttt cttcttcgtc agtgctcgga tctgtgtgtc aatttgcgtc
      481 agtaagtcgt tcgagaaaat ccccttagta gtcctaaagg aataaccgga tagctcgcag
      541 gccgattgtt tggcacccta ctgcaggccc aaagggacaa ctcactcccg ttgggcttgc
      601 aggaaaaacc aagtgggagg ccctgatgaa atggagtcct gttaaggttg ttcgtaacac
      661 actcctagca ttatcggtcg gtgtactctt gccctgcatt gcctcggcga gcagtgtggc
      721 gccgaatgca atgcccacaa ataacaacac agccggcgtg tggcttttca ttccagacgc
      781 tacggaatcg ctgatgaact cggccacgct tttggggtcg attccattcg gcaaagattc
      841 gctcgacatc ttcaagatca gcgacggcca ttttgccctc gcctttgtcg atgcagatgg
      901 cgacgattcg atttcttccg tgggttcggt gacgtcaacc gcaccggagt ctgatcctgt
      961 ggctaccgac gactcaacac caacaaacac ccccgagcca tcttcattgg cgctgcttgg
     1021 cctcgggaca atcgctttgg gactggcttt tgccggccgt cgcctcaaac ccacgacggc
     1081 tggatagctt ccctctgcga agtaaaggac tcagcggcct cagcccgctg ggtctgcagt
     1141 acctcgtccc cgcaatccct ttcctctcag ccattgagaa aagactgcgc cccgcggctg
     1201 cgcagcacga atgtcgggcg gcataaattc gatccaacac tctcagcttc aatacaaaat
     1261 ccggacagtc ggatctctgt ctaaggaggg gttcgagaga atctgtcagt caattcccgt
     1321 gtgatatcat ccgtccgctc tgtaacgtca actgcagaaa atacggagag cgacaatccg
     1381 tcagcacctt cttgggaggc aagatgaaac tggttcgggg agttgtcttc gcatcggcat
     1441 tatttgtcgc gttcggaatg ctggcgcgag ctcaacgagg tggcaaacgg actgcttccg
     1501 ctgccgcgcc gcgccaatct ccccagctat ttacagaacc agttctggtg aagggctctc
     1561 ctacgaatct gcgtttcttc gacgtgagct ggaacgacaa ttccaaacag aggtactacc
     1621 tggcggatcg cacgaacaac gcgatcgacc tcattgattc ggccacggac acgtttctcg
     1681 gattcatcgg caagggccat tacacgggca gcaggccttg tccgaacaac cccaaagata
     1741 tgcgcctctg cgctggaccg gacggagtgg tgacggacga tctcggccac gtatggtctg
     1801 gagatggcga cgcaaacatc atcgaagcgg acgacaaacc gggtagcacg atcatccggt
     1861 cgatcccgac cggcggcaag ggccggattg atgaattggc ttacgatccc atagatcgga
     1921 ttctcttggc cgagaacgat ggcgattcgc cgccgtatgt gattttcgta tccgtgcccg
     1981 gtggcaccgt gctgggacgc tacgtgtatc cgaagagcca ggatggcatg gagcagccgg
     2041 cctgggttcg cgagaccgac atgttttatc agaatgtccc tgggccgaag aatcgtctcg
     2101 atgtgttcga tccgcatcat ctccccaagc cggtcagggc ctttccggtg gactgcacgg
     2161 gcggattgct cggccttacg ctgagcggtc tgaccgttgg tccaaacgcg cgcctgatga
     2221 cggtgtgcgg cagtgtgggc ggcgtggcga ttgactcaag gaccgggagg gtcggaaaac
     2281 ctatccccga aggcgccgac gcggacgagg tttggtacga cccgggagct ggcaactact
     2341 atttctcgcg aaattcgaac ctggctgtcg tgaacgcctc gaccgaaaag tttgttacgt
     2401 acgttccctt ggggagccat tccgtggccg caaatgcgaa gaacaaacac gtcttcgtcc
     2461 cggttgcagg caagggcatt tttatggtgg cgccaaggca gtgaagtagt ccggcagatg
     2521 gttgtttatg cggcggtctt acatctctga acagcgagta ggaattgccg tcgattcatt
     2581 gcagcacctc taatccgacg cgccccctac ttatagaaca ggcgcggact gtgaatgact
     2641 gaccgataag cgccgcagaa tttacgatta gccggatggc tagaagcatt gcgcgctcag
     2701 gccacgcact gaagtcgccg ggcttttttt cctaggcagc gtcccactca aatagggcat
     2761 catgccaatg aaattagctg tccgggaagt tgacagcctg gtgttaccgt cataacatca
     2821 tcctgaaatg gccatagcca gagttgtgga cggcgcggcg tggctgcttc tgacttttac
     2881 gttgccgatg aaatcggcca gccagagagt tcaggtgtgg cgcaaattgc agcgttacgg
     2941 cgctatcccg cttggcaact ctggctattt gcttcccaac aatccggcag cccaggagcg
     3001 ttttgagtgg ctagcaactg ccattcgcaa atacgcgggt gacgcctcag tcgtgaaagt
     3061 gcaatccatc gacaacctgt caactcccca gttggtgggg cggttcgccg aggcgcgtgg
     3121 gcgcgactat caggaattaa tccgggaact gcgcaagatt ttgtcgggtc gagaacgcaa
     3181 gagcacaccc ggccgtttga gccgcctgag agcgcgcttc cttgaaattg cgcaggtcga
     3241 tttcttcaac agccctctcc aaaagcgagt cgaggaactt ctggcgcgcg ccgacgcaag
     3301 cccagctccg acagaagaaa cggtcaaggc tgatccacgc gaatacgtgg gcagggcgtg
     3361 gatcacgcgg ccgcgacctg ggattgaccg ttcggcttcg gcctggctga ttcaacgttt
     3421 catcgataag aaggctcgat tcatttttgg tcctgaagac cgtgtgcctc gcgaagcggt
     3481 gccttatgac atgtttcacg gtggatttgg tcaccgtggt gaagattgca cttttgaaac
     3541 gttgcaaaaa cagtttcgga ttcatgaccg taaagtgcgc gtgatcggcg aaatgattca
     3601 cgatgcggac ctttttgacg agaagttcgg ccggagagag ggcttcggca tcgacgaggt
     3661 gcttaacggc tgggcgaaac agggaattcc tgatggcgag cttttgaagc gagggatgca
     3721 gcttatcgaa gcgctgtatc actcgctttc gggaaagtaa ttgtgagtgg gtgcgatgcg
     3781 ttctgcagct tggatgctct tttatggagg aatctcatgc gacgtttatt gatggtgatc
     3841 tcgatcctgg tgttccccat tgtggtatcg gctcaggaac tcgacatagc gaagatcgat
     3901 caagtgctcg gcaggtcggg ccagaagact ggtgacgttt acaaagttgg cttcccgcga
     3961 acagatttgc acgttgttgt ccatggggtg gcaatcaagc caggattcgc cctcggatca
     4021 tgggccgcgt tttcgggaag cagcgcgaac gctcgcgtga tgggcgattt ggttttactc
     4081 caggacgaag tgaatccagt gatggagaag ttgcgctcgg ctggtttcga gattacagcg
     4141 gtccacaatc acttaattga tgagtctcct cgcgtgatgt acatgcatta catggggcat
     4201 gggccggcgg cccagctggc cagctctcta cgcgccgcgc tggctgtttc caagacaccg
     4261 ctgggaaaac ccgctgccac ggcggaacct tcgtctgctc ccgcctgggt gaaaacggtg
     4321 gaagatgcgg ttggacgcaa aggcagcttt aaaggcgggg tacttgcgtt tggcattccg
     4381 cgcgcgaatg ccgttacgat gggcgggatg acgatcgcgc cggccgaagg tgtcgccgag
     4441 tccatcaatt ttcaagacgc cggtccagga aaggtggcaa cgacgggaga tttcgtaatg
     4501 actgcggagg aagtgaatcc ggtgatttcg acgctcgaac aacaccatat ttcagtgatg
     4561 gcgctgcata gccacatgct cgcggagcag ccacgtctct ttttcatgca cttttggtcg
     4621 gtgggaagcc ccgggtcggt ggcggaggga attaaggctg ctttgagtca tatctcgacc
     4681 aaataggctt ggccatctgc ggcgcgatat cacgttacgc cttggttggg ggaacatatg
     4741 cgggccgaac acttatcttc gaattggcaa gtgctcggcg tagttctgct gctcctcttt
     4801 tgcgcgaacc ggacgacagc ccaaggcggg ccaccgcttc tgacaaatga tcctgttaca
     4861 cccgatcatg ggaactggga aatcaatctc ggcgccatgc ctgtgctgac gaaaatcacg
     4921 aaatcagtag aagtcctgca gattgacctg aactacgcat tgaccgaccg gattgaattg
     4981 atctacgaag tgccctttgt ttggcaaagc gcaactggac agcccaacgc gacgggatgg
     5041 ggtaatggat tgcccggtgt gaaattgcga ctcatcgatg gtggcgaaaa tggctggaat
     5101 ctttcgactt tcccgcaagt ccaaattggc ggttcggcgg cctccgtgaa gaaaggcttg
     5161 gccgacgagg gcacgcgatt tcttctacct tttgaggtga gcaagcggtt tgggccgttg
     5221 aatgccgatt ttgaagttgg gtacttcttc ccgctcgatt cgtccgcttc ccgcgacgag
     5281 cggattctag ggcttgcgct gggtcataag ttgacgccga aattggagac aattggcgag
     5341 atatacgatg acaaagtcat gggagcaccc gaacacaaca cggaatttga tatgggaggg
     5401 cgctgggaat tccacaaggg gctgcttttc ttgttcatgg ctggccgcag tttcaagggc
     5461 aattcgagcg gccaaccaga gttcctgggt tacggcggga ttcgaattct ccttgaccac
     5521 tatggaacgc gtttgcattc cgataagtga gtgcgatcta agtagccgtt caatcaacgc
     5581 ttcaaccgtt tgtgatttct tccagatttg tttcggctag gttgtgctat atgtttcccg
     5641 agtgggcata ctggggagtg cgacgtgaaa acttggaaag cgtttgtcct cggattgatt
     5701 gcggccgttg tggtcgctgc gattggcggg attttattcg ttcaccgcgg ttttcgggcg
     5761 acgaatcaac cgttaccgct tgaggctgtg gtggctcgag ccgcgcgaaa ctggtccatt
     5821 cccgggcggg cgcggaacga caaaaatcct ttaaagctta cgtcggagaa tttgcgacag
     5881 gggcgcgaag aatttttagc tcgctgcgca agttgccacg ggcatgacgg cagcgggctg
     5941 acggatatgg gccgaagtct ctatccgagg acgcccgatc tgcgctcgaa tgaaacgcag
     6001 aatctgagcg acggcgagat tcattacatc atcgccaacg gagtgcagct taccggaatg
     6061 ccggcgtgga gcaatccgca ccaggaacga aacgtcaacg cctggagcct ggttctcttc
     6121 gttcgcagcc tgcgcccact gacgcgcgaa gagaaggcac aacaggcaag cacggcaaag
     6181 tccgcacact acgttggctc gcaagcgtgc gagaagtgcc acacacagat atacgaccat
     6241 tggaaaaaga ctccgatggc taacatcgtg cgcgatccgc gcgagcatcc ggatgcgatt
     6301 attcccaacc tggcgacaaa caacgtatac aagttcacga aagatcaagt tgcttttgtt
     6361 tacggaagca tctggaagca gcgctatttc acgaaagtcg gtgacgatta ttttccgctg
     6421 ccggttcagt gggaagtcaa caatcacaaa tggagcaaat atttcgcggg ccctaaagcc
     6481 gattggtgga cgaagtttta tcctcccgac aatatgaagc ggcccaccgg ccccacatgc
     6541 gacggctgcc attcggttgg gtacgacatc cacaccaaga aagtggcgga gtggaatgta
     6601 ggctgcgagc gctgccacgg tccgggcagc gagcacttgg cagatcctcg gcgcgacaac
     6661 atcgtcaatc cggcgcacat ggactacgtt gcggcgagcg atacctgtat ccagtgccat
     6721 tcgcaagggc gtccgctgac gatcccgatc gaggggaaat attacgattg gccggtgggt
     6781 taccaactgg gactgcggct gcaggatttc tggaagctcg aagaccacac gctggccgag
     6841 accaactttt attatttcgc ggacggcaca gcacacaaaa atcggatgca aggaaatgat
     6901 ttcatgcaga gcgtgatgta ccgccgcggg atcacctgct tcgattgcca cgacgtacac
     6961 ggcacaaaca actccgcgca actgagagag tccgccgata agctttgcct ggaatgccac
     7021 ggaccgcttt cgcccaacgg gccgcgaacg gcgacactcg aagaacacac gcaccataag
     7081 gagggcagcg ccggaagcca gtgcgtggct tgccacatgc cggcgattga aacggaaggc
     7141 ccacccaaca caatggttca cgcacatacg ttccggttca tcaccccagc gatgacggac
     7201 aaatacaaga tcccgaatcc atgcacgtca tgccacaagg ataaatccac agcgtgggcg
     7261 accaatgaaa tgagcaaatg gcccgagcgc tcgccgtggc ggctcgagta ggagtggcta
     7321 tttcgctgtc gtgtgagcgt tcgcatcccc aggtgatgcg aggagcacgc gcggcaaaca
     7381 gcgggagccc gtcgaggctc ccttcctgcg tcagggcaag caggggaaac aactcccgca
     7441 ctccatattg acctgatgga aagacaattg ctcatttaac ctcagctggc ggattctgct
     7501 gagggtgaag catcagctaa gctacgtgtt tctttcggtt tatcgttccg gcgctggccg
     7561 gtcaatgtgc aaagggtggc acctctgcgg aacggaactg ttctcgaaga gcggcggaag
     7621 tacgtccggc accggttaac ctcgaccgtc tatgtggagc ttggtccagg gaatggcggg
     7681 atcatcgtca accttggcac gggcggattg tccatgcatg cagcggtcaa gctgaatgct
     7741 gcaatggaat tggcgctgcg tttccgactg gaccgcaccg aggaaccgat tgaagtagcg
     7801 gggcgggttg cctggctggg tccgacgcag aaggaagctg gtatcgcgtt cagcgacctt
     7861 ccgagcagcg cagaaccgcg gatcgcagac tggattgcag agcaggaaaa cccggcctcg
     7921 gacacgcaac gcgatttaga gcctcagtcc aagtcgtccc tgacgaagag cgcagcgccc
     7981 ccgcttccca ttcgagcctc tgccgcggag agtctcgtgg ctcaaaaatc tgagagtgta
     8041 ctgccgaggt cgagcatggg cattccgcac ttgggcactt cggaatcttc ccaactgcat
     8101 aattttttga gtacgacgcc cgatttgcct gagacgcctc ctgcaattgc ttttcgaacg
     8161 actgaagagc gattcgaaaa gccgtccgac aagctcctgc gatcgcctgc taagcgttat
     8221 gaggtgctac tcgagcctga gaagccgatg ccggccgcga aagaaatcct tccacaagag
     8281 accgcgtcgc ccagcttgac gcctgccagc agcaaagtta ttg
//
LOCUS       DATNDT010000399         3237 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1815312, whole genome shotgun sequence.
ACCESSION   DATNDT010000399 DATNDT010000000
VERSION     DATNDT010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3237
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1815312"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="VN788_12990"
     CDS             complement(<1..468)
                     /locus_tag="VN788_12990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase N-terminal-like
                     domain-containing protein"
                     /protein_id="HXU21491.1"
                     /translation="MREAVIVSSVRTAVGKAFKGTLRAARPDDLAATVMREAIARVPG
                     LDPKEIEDVILGCAMPEAEQGMNVARIASIRAGLPVEASAMTINRFCSSGLQSIAIAA
                     QRIMAGQGEVILAGGVESMSLIPMGGNKISPNPWLMDHYPDTYLGMGLTAENLS"
     gene            977..1426
                     /locus_tag="VN788_12995"
     CDS             977..1426
                     /locus_tag="VN788_12995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21492.1"
                     /translation="MTMWLYQMDQQQWPPNSYRLDIWERERWTWPVGRKASTDSPKPG
                     DRVVFFYAPSGGIEPGFYGWALVLDWHEDERGMYFRPVAPSDHLKMHPWWDTAAKEIA
                     DAVRGAVKQGTLWEVPDKLASEISARITGWLSASAEDVKAPSQRRNT"
     gene            1579..2688
                     /locus_tag="VN788_13000"
     CDS             1579..2688
                     /locus_tag="VN788_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016050567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P63C domain-containing protein"
                     /protein_id="HXU21493.1"
                     /translation="MDTTEKQLTERESNYPSDAGKALSDLGASKGGEARALKLSPERR
                     REIARAAVAARWAKAGKNPVPQATHAGQLKIGDLSIPCAVLDNGTRLLTQWGFYRAIG
                     RSGRPAAGWGSDVEKVAPFLSLDNLKPYISDELAASKPIQFRVARGGKAWGYRAELLP
                     KVCEVYLKAREDGALLKSQLKFAKACEILTRGLAHTGIIALVDEATGYQDDRAKDALA
                     KILERFIAKELRPYVRTFPTDFYKEMFRLRKWRWPDLPADQRKRPILVGRLTDNVVYD
                     RLAPGVKQRLKELVGRDESGRLKHKMFQRLTDEIGDPKLREHLASVVALMKAADDWLQ
                     FIVMIDRALPRYKPLPLFDPVPELEACGIAISSKF"
     gene            complement(2685..>3237)
                     /locus_tag="VN788_13005"
     CDS             complement(2685..>3237)
                     /locus_tag="VN788_13005"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21494.1"
                     /translation="VVAFQARDIREAVEFCEKQKVRMILAGASEAWKTIDLLKKDNIP
                     VIIGPVLSMPQSEDDPYDSEYTMPGQLYAAGIKIAFGSFNTSFSRRLPQYAGTSVGYG
                     LPHDEALKAVTFNAAQMLGLGDQLGTIEPGKIANLVVTDGDLLELRTQVKYLFIQGRQ
                     TSLDNKQLELYERFSHRPKPESH"
BASE COUNT          750 a          845 c          951 g          691 t
ORIGIN      
        1 cgacagattt tccgccgtca atcccattcc caaataagtg tcgggataat ggtccatcag
       61 ccacggattc ggcgaaattt tatttccgcc catgggaatc aggctcatgg attcgacgcc
      121 gcccgcgaga atcacttcgc cctgtcccgc catgatgcgc tgcgcggcga tggcgatgga
      181 ttggagtccc gaggagcaga agcgattgat ggtcatcgcg gacgcttcga ccggaaggcc
      241 cgcgcgaatc gacgcgatgc gcgcgacgtt cattccctgc tccgcttcgg gcatggcgca
      301 acccaaaatc acatcttcga tttctttggg atcgaggccc ggcacgcgcg cgatcgcttc
      361 gcgcatcacc gtggcggcca aatcatcggg acgcgccgcg cgcagcgttc ctttgaacgc
      421 tttgccgact gccgttcgca ccgagctgac aattacggct tccctcatgc tgcacctcga
      481 aaacaatcag gtgagaattt gctgcgcttg tccctgttat tggacgccac gagacgagaa
      541 aaagcaacag cggcggggcg tttgaacgcc tcttgcgagg aacgagtggg atatgaaaat
      601 cgggagaccg aaaaaagcgc gagcgcgcaa aaacgaaact cattaccgat caaaaaaatg
      661 taagtgacag attctacgcc agttgaattt gcgcgactgt gcatgtcatt accgctcaaa
      721 aaaatcatag attggggcgg gagacccggt ttggcgggga aaagaggcgc tagacggaac
      781 acagactccg ccgtcgccct gttaggaggg cgagggagat agagaagcgc gtggccataa
      841 tcatatagta actatatcac acattgccgc ataatgtaaa ctggcttggc atcatggtag
      901 tgctctgatt gatttcgtgc cgcggaaatg tatactgggc cgagattagg acttttcgcc
      961 agtccggggc gcgacgatga ctatgtggct ctaccaaatg gaccagcagc aatggccgcc
     1021 aaacagttat cgactcgaca tttgggaaag agagcgttgg acgtggccag tgggcaggaa
     1081 agcatcaacc gacagtccaa aacctggcga cagagttgtc ttcttctacg ccccgagtgg
     1141 cggcatcgaa ccgggattct acggctgggc gctcgtcctc gattggcacg aggacgaacg
     1201 cggcatgtac ttccgacctg tcgctccaag cgaccacctc aagatgcatc catggtggga
     1261 cactgctgcg aaggaaatcg ccgatgccgt gcgcggcgcg gtcaaacaag ggacgctgtg
     1321 ggaggtgcct gataagttgg cttcggagat ttctgcgcgc atcactggtt ggctgagcgc
     1381 ttctgccgag gatgtaaagg cgcccagtca gcggcggaac acctgatatg tgtttcctcc
     1441 cttccactga agatcccacc ggaaacggct atctcgacag tcagctgtgg aggaaacaaa
     1501 agacgacagt catacttgcc cgtgcatgaa gatcagattt cgcttgactt ctttctcggg
     1561 caagtatagt tttctggaat ggacaccaca gagaagcaac tcacggagcg cgaatcgaac
     1621 tatcccagcg acgccggcaa ggcgctgtcc gacttagggg catcaaaagg cggagaagcc
     1681 agggctttga aactttcccc tgagagacgc cgcgaaattg cccgggcagc tgttgcggca
     1741 cgttgggcaa aggctggcaa aaaccccgtt ccgcaggcta cgcatgccgg ccaactaaaa
     1801 atcggcgatc tctccatccc ttgcgcggtt cttgataatg gaacgcgctt attgacgcaa
     1861 tgggggttct accgagctat aggacgatct ggcaggcccg cagcgggatg gggatcggat
     1921 gtcgagaagg tcgcaccatt tttgtctttg gataatctta agccctatat atccgatgaa
     1981 ttagcggctt cgaaaccgat tcagttccgc gttgcacgcg gcggaaaagc atggggctat
     2041 agagcggagc tgttgcccaa ggtgtgtgag gtttacctta aggccaggga ggatggggcg
     2101 cttctaaaat cgcagctgaa gtttgcgaaa gcgtgcgaaa ttctgacgcg cggccttgct
     2161 cacaccggaa tcatcgcact cgtcgatgag gcaacgggat atcaagatga tcgagccaaa
     2221 gatgctttgg cgaaaattct tgaacgattc attgcaaaag agcttcgccc ttatgtgcgc
     2281 accttcccga ctgactttta caaagagatg ttcagactga gaaagtggag gtggcctgac
     2341 cttcctgccg atcagcgcaa gcgtcccatc ctagtcggta ggctcaccga caatgtcgtt
     2401 tatgaccgtc ttgcgccggg cgtaaagcaa aggctaaaag agttagtcgg gagagacgaa
     2461 agcggccgcc taaagcacaa aatgtttcaa cgattgacag atgaaattgg ggacccaaag
     2521 ctcagggagc atcttgcttc cgtggtagcg ctcatgaagg ccgccgacga ttggttgcag
     2581 tttatcgtga tgatcgatag agcgttgccg cgctataaac cgttgccact cttcgatcct
     2641 gtacccgaac ttgaagcatg tggtattgct atcagctcca aattctagtg cgattcgggt
     2701 ttggggcggt ggctgaagcg ctcgtagagt tctagctgtt tgttgtcgag gctggtttgc
     2761 cggccctgaa tgaacaggta tttcacctga gtgcgcaatt cgagcaagtc gccatcggtg
     2821 acgacgaggt tcgcgatttt gcccggctcg atagtgccga gctgatcgcc gagcccgagc
     2881 atttgcgcgg cgttgaacgt gacggccttg agcgcttcgt cgtgcggcaa gccgtaaccc
     2941 acggaagtgc ccgcgtactg cggcaagcga cgcgaaaacg aagtgttgaa gctgccgaac
     3001 gcgatcttga tgcccgcggc gtaaagctgg cctggcatgg tgtattcgga atcgtacggg
     3061 tcgtcttccg actgcggcat gctgaggacg ggaccgatga ttacgggaat gttgtctttc
     3121 ttcagcaggt cgatggtttt ccaggcttcc gaagcgccgg cgagaatcat gcggactttc
     3181 tgcttttcgc agaattcgac ggcttcgcga atgtcgcgcg cctgaaacgc gacgacc
//
LOCUS       DATNDT010000400         3860 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2380282, whole genome shotgun sequence.
ACCESSION   DATNDT010000400 DATNDT010000000
VERSION     DATNDT010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3860
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2380282"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="VN788_13010"
     CDS             complement(<1..974)
                     /locus_tag="VN788_13010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HXU21495.1"
                     /translation="MPQYIIGIDLGTTNCAFACAEPAAAPDPHEMPPVHLFPVAQLAN
                     PGEVREEPLLPSFLYLPGPNDFPAGTLELPWGTGDPLTGQLAQKRGAESPSRAISSAK
                     SWLSYAGIDRTAPLLPIGAPDGVRKLSPVEVSRQYLDHLRNAWNHTHPEAPFEQQQIL
                     VTVPASFDAVARELTLKAAEQAGYANLLLLEEPQAAFYAWIERHPDWRDRVQVGDLIL
                     VIDIGGGTTDFTLIAVTEQKGELQLDRVAVGEHILLGGDNIDLALARHVEQQLSAKGT
                     KLDFLQLYALWQQCRLAKERLLDNENVGKADQAVTILGRGTGVVGGTI"
     gene            complement(979..1497)
                     /locus_tag="VN788_13015"
     CDS             complement(979..1497)
                     /locus_tag="VN788_13015"
                     /inference="COORDINATES: protein motif:HMM:NF022273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2760 domain-containing protein"
                     /protein_id="HXU21496.1"
                     /translation="MSRISFAFRAFFALLFGRGLPEDIAQAYGFSKPKPLPVVKPVPQ
                     LSPADGALQILGILQRDARLIDFLMEDIGPYSDDQIGAAVRPLHDQSREALNRYLRLS
                     PVIDGVEGTFTKADSSDPASVKLLGNVPAKGKAPGGLLRHKGWRAEKVELPPLAASQK
                     SAIIAPAEIEVE"
     gene            complement(1802..2215)
                     /locus_tag="VN788_13020"
     CDS             complement(1802..2215)
                     /locus_tag="VN788_13020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21497.1"
                     /translation="MSRVSLEANPARERKAIWGRKDEEYVADFCLVTRRVLSDSEYRI
                     FKFHFLLGADWKLCCRRLGMDRGNFFHAVYRIQQRLGRVFRELEPYSLYPLDEYFNGT
                     TAPAPAFNAPRANVIPIRPPLALRSSFPMPLRKTA"
     gene            complement(2973..>3860)
                     /locus_tag="VN788_13025"
     CDS             complement(2973..>3860)
                     /locus_tag="VN788_13025"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HXU21498.1"
                     /translation="SERSLLAGNVTIQRIGYNQQSDFGSILSQSATPAKTPTAPAGPV
                     AGMRLDVRIRTAPDVKFQTSLARQLQGQADLRLRGTLANPGMTGRVVVTSGTLVFFGN
                     QYNVNRGNVAFYDPLKIEPILDVSLETMAQGVDVILGVTGPIDNLKLTYRSDPPLEFQ
                     QIVALLATGRTPADPTIAANQPAPPQQGLAQMGESAIVGQAIANPIANRLQRVFGVNQ
                     ISISPTFVTGSSIPQARFTLQQQVTPTITFSYTTDLTETNSQIIRIEWALTPRFSAVA
                     TRDENGIFGLDFYYKKHFR"
BASE COUNT          834 a         1004 c         1215 g          807 t
ORIGIN      
        1 ttgatcgtgc cgcccacgac accggttccg cggccaagaa tcgtcaccgc ctgatcggcc
       61 ttgcccacgt tttcgttgtc cagcagccgt tccttagcca gacggcactg ctgccatagc
      121 gcataaagct gaaggaaatc gagcttcgtg cctttggcgg aaagctgctg ctcgacgtgg
      181 cgcgcgagag cgaggtcgat gttatcgccg cccagcagga tgtgctcgcc gacggcgacg
      241 cggtcgagtt gcagctcgcc cttttgttcg gtcaccgcga tcagcgtgaa gtccgttgtg
      301 ccgccgccga tgtcgattac aagaatgaga tcgccgactt gaacgcgatc acgccagtcg
      361 gggtggcgct cgatccaggc gtagaaggcg gcttgcggct cttcgagcag aaggagattc
      421 gcgtagccgg cctgctcggc cgctttaaga gtcagctcgc gggcgacggc gtcgaacgat
      481 gctgggacgg tgaccagaat ctgttgctgc tcgaacggcg cttccgggtg ggtgtgattc
      541 caggcattgc gaagatggtc caggtactgg cgcgagacct cgaccggcga aagtttccgg
      601 acaccatcgg gcgcgccgat tggcagaagc ggggcagtgc ggtcgatacc ggcgtaggat
      661 agccaggatt tcgcggagga gatggctcgg ctgggacttt cagcgccgcg cttctgggcg
      721 agctggccgg taagcggatc gccggttccc caaggaagtt ccagcgtccc ggcgggaaaa
      781 tcgttcgggc cgggcagata cagaaaggag ggcaggagcg gttcttcgcg gacctcgccg
      841 ggatttgcaa gctgcgcgac cggaaacaga tggaccggcg gcatctcgtg cggatcgggc
      901 gcagcggccg gctcggcgca ggcgaaggcg cagttggtgg tgcctagatc gatgccaatg
      961 atgtattgcg gcaaattgtt actcgacttc gatttcagcc ggagcgatga tggcggactt
     1021 ctggctggcc gcaagtggcg gaagttccac cttttcggcg cgccaccctt tgtgacgcag
     1081 caatccgccg ggggccttcc ctttggcggg cacattgccc aacagcttga cagacgcggg
     1141 atcggacgag tccgccttgg tgaacgtgcc ttccacgcca tcgataacag gcgagagccg
     1201 cagatagcgg ttcagcgctt cccggctctg atcgtgaaga ggacggacgg ccgcgccgat
     1261 ctggtcgtcg gaatacggcc cgatgtcttc catgaggaaa tcgatcagcc gggcatcgcg
     1321 ctgaagaatt ccaagaatct gcaacgcgcc gtccgccggg ctaagctgcg gaactggttt
     1381 cactaccggt agcggcttgg gcttcgagaa gccataggcc tgggcaatat cctcgggcag
     1441 gccccgaccg aagaggaggg caaaaaaagc tcgaaaagcg aaggaaattc gtgacaagcg
     1501 aaacctctaa gtctttagga tacaacgcgg aggggattga gtgattgggc gattgagtca
     1561 attgagtcat tgggagatcg gggaattggg gactcgggac tgggagattg cccagaggat
     1621 tgggagattt ggacgggatt aggggattga gcggtttgga attgggcgat tggaattggg
     1681 agcgaggatt tctggttggc cgttgcgagt tgggaactgg gcggccgagc gattgaggat
     1741 gcggccggaa tggaaaaagg tccgccgccc ccaaggatgg gcggcggacc gaggggaggc
     1801 gtcaggcagt ttttctaaga ggcattggga aagaggagcg gagcgcaagc ggcggccgaa
     1861 tcgggatcac attcgccctt ggcgcgttga acgccggcgc gggcgccgtg gtgccattaa
     1921 agtattcgtc cagagggtaa agcgagtaag gctctaattc gcggaacact cggcccaatc
     1981 gctgctggat gcggtagacg gcgtggaaaa agttgccgcg gtccatgcca agacggcggc
     2041 aacacaattt ccagtccgct cccagcagaa aatgaaattt aaagattcga tactcagagt
     2101 cgctgaggac cctccgcgtc acaaggcaga aatccgccac gtactcttcg tccttgcggc
     2161 cccaaatcgc tttgcgctca cgagccggat tggcctcgag cgaaacccgg ctcatgctct
     2221 tttctttccc ggcgcaatag cgaaaacgtg aaaagcaagc acgaaagatt gcccgaagca
     2281 cgcaatggca cggctcttgg gaactgccgc ggctgtttcg aagtcctagc ccccggcaca
     2341 cggcacaacc gggagcggcc aaagcgattg tatctgatct agtccattcc atagtcaagt
     2401 tgttaggtcc tcttctccca gcaagctttt tttcgttgtc tgggcgtttc atctgtgtcc
     2461 atattaggcg tacggccgag atcgtcaacc gggcagagct cgatttctgg ggatcaaata
     2521 cggttcgaaa ggagcagata gtactaaaaa ccgatttaga tcaattattt ggagattttt
     2581 agagaggtgg gaaaattttc gcaatccaac tgaaaattat gcacgtggtt tcttaccatg
     2641 agatgatcta tttcaaggtg aaatagagag gtataccctg ctctccttta ggaagcttca
     2701 ggaaaggctc gttgcgcaag tacaggaaca ggtgcgcaat ggagatatta ctgagcgaaa
     2761 attggctcgt ttaaccggat tctcacaacc gcacatacat catgtgctaa aaggagcaag
     2821 aactctcact ccggttgtgt ttgatgcgtt actcgaggaa ttggacatgt cgctgctcga
     2881 cctatgcacg cccgaggagt tggaagctga gcttcggaaa aggcggaccg accaaaaacg
     2941 agtataaatt tacccggaat attcttgcga catcagcgaa aatgcttctt ataatagaaa
     3001 tcaaggccga aaataccatt ctcatcacgg gttgccacgg ccgaaaagcg cggtgtcaag
     3061 gcccactcga ttcgaataat ctgagagttt gtctccgtca gatctgtcgt gtagctgaag
     3121 gtgatggtcg gtgtcacctg ctgttgaagc gtaaagcggg cctgtgggat tgatgaaccg
     3181 gtgacgaaag tgggtgaaat cgagatctga ttcactccaa acactcgctg taaccggttt
     3241 gcgattggat ttgcgatcgc ttggccaacg atcgccgatt cccccatttg ggccaaccct
     3301 tgctgcggtg gagcgggctg gtttgcggcg atggtcgggt ccgcgggcgt tcttccggtt
     3361 gcgagcagcg ccacgatttg ctggaactct aacggcgggt cggaccgata ggtcagcttc
     3421 agattgtcga tggggcccgt gacgcccaga attacgtcca cgccttgcgc catcgtttcc
     3481 agactgacgt ctagaatcgg ctcgatcttt aacggatcgt aaaaagcgac atttccgcgg
     3541 ttcacgttgt attgattgcc gaaaaacacc agcgtgccgc tcgtcacaac gactcgtccc
     3601 gtcattcccg gatttgccag cgtgcctctc aagcgcaggt cggcctgccc ttggagctgg
     3661 cgcgccaatg aggtctgaaa ttttacgtcg ggcgcggtgc ggatgcgaac gtccagacgc
     3721 atgcccgcga ccggtccggc cggggccgtg ggcgttttcg cgggagtggc cgattgagac
     3781 agaatggagc cgaaatcgct ctgctggttg tacccgatac gctggatcgt gacatttccg
     3841 gctaacaggc tgcgctcgct
//
LOCUS       DATNDT010000401        19572 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3315255, whole genome shotgun sequence.
ACCESSION   DATNDT010000401 DATNDT010000000
VERSION     DATNDT010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 19572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19572
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3315255"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..354)
                     /locus_tag="VN788_13030"
     CDS             complement(<1..354)
                     /locus_tag="VN788_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twitching motility protein"
                     /protein_id="HXU21499.1"
                     /translation="MDETQQPSPKAEKATPAQPLPTPSIIAAMLRASPKISDLIFSPG
                     RAPQIEANGQLVQLNIPGVGRLGSEDTARIAADLIGRNSHAVDKLKQEGSCDISYSLP
                     KISRFRVNVFTQRGSC"
     gene            814..1584
                     /locus_tag="VN788_13035"
     CDS             814..1584
                     /locus_tag="VN788_13035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU21500.1"
                     /translation="MPGTAADPFDDLYGTPPMAVMPSPSDGLIESEGCNSWTESGVRS
                     PTVSVARLAVPGKASGEYQKGCGAYKDKRFDQAERHFRKAIDIYPSYPAAWVVLGQAL
                     DAQHKRDDARQACSQAIKIDPTYVAPYLCLAEFSATEDHWKQVANLSKRALAIDPVNN
                     PYSLYYTADAAFHLNNLAEAEAKALAAVALDPWHHLPQLHLLLAQIYAAKGDLDAEAA
                     QLRNYLKVAPNSGDAAGARDTLAQLQSRPAAAKADPQR"
     gene            complement(1581..3242)
                     /locus_tag="VN788_13040"
     CDS             complement(1581..3242)
                     /locus_tag="VN788_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HXU21501.1"
                     /translation="MEHSPSSASSGMGQGPQNGGSQARGIALLVVVLAVSALLGGFVY
                     GPQARATAAGSDDAQDSVKSFTHVLSVVQRNYADPVDTDKAIYDGAIPGMLHVLDPHS
                     NFFDPKQYALFREEQEGRYYGVGMVVAQRDNNTVVQSPFVNSPAYKAGIRPGDIILKV
                     DGKTCTGLTTTEVAEMLKGAKGTPVHISLGREGWEKPIEVTIVRDEIPRPGVQFYEMV
                     KPGIGYVRVSTFNETTDSDLSDALKHLDVSKLDGLIIDLRGNGGGLLNQAVGMADMFL
                     DKNELVVSHRGRSSPERPYYSARGNQGVEVPLIVLVNGQSASASEIVSGAIQDHDRGL
                     IVGETTFGKGLVQTQFPLSENTALLLTTARYYTPSGRLIQRNYKNVTLYDYHYNPQRP
                     KAPEVKLTDTGRQVYGQGGITPDVVVAAPKPDDFEDLLERRGIFYPLPEGVGDFARFY
                     LGQKPNVTKDFVVDDAVIAQFKKYLAGQNIRVTDQQIEQNLPWLKWEIKREVFTTLFG
                     LNEGYKVELDNDPQLVKAEESIPQAKALYANARKIVAERQSSTQP"
     gene            3320..3847
                     /locus_tag="VN788_13045"
     CDS             3320..3847
                     /locus_tag="VN788_13045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXU21502.1"
                     /translation="MMQKERKWALVLRLPMLSIPHLIIIFVVALVIFGPEKLPELARN
                     LGKVMAEFRRHTSDLRTTFEAHLRDIEREGESRRIAAVPAPVAQPPAAEPPAPASPGT
                     VPSKPPNAAAIAAPSDDPFSVDVPDPDLRRKDPGDDPYLSGTEFDPGGPPEETQATEP
                     EKNSERVENGGVRPS"
     gene            3825..4661
                     /gene="tatC"
                     /locus_tag="VN788_13050"
     CDS             3825..4661
                     /gene="tatC"
                     /locus_tag="VN788_13050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HXU21503.1"
                     /translation="MAASAPPELTPRQHAEEESGAAMSFFDHLVELRKRIISALIAVG
                     IGMAIGLTVARRFINFISQPMWVALRANHIDPNLYYTHPAGYVSLYISIGLYLGIALA
                     MPWVLYQVWLFVAPGLYKHERRAVASFIGSSMFLFLCGIAFGYFVMLPQVLTYVVAFA
                     NGGSVGGSIKPLIDINEYFDLILVVLVGLGVIFELPVVVFILSLFGIVTPKFLMRNFR
                     YALLLITVAAAIVTPTPDATTMLIFMAPMVVLYFIGVLVSYAVLRNKRAKELAEGQAP
                     PG"
     gene            4658..5680
                     /locus_tag="VN788_13055"
     CDS             4658..5680
                     /locus_tag="VN788_13055"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HXU21504.1"
                     /translation="MTRVLRLRWGGVAALALLLSASCGHGGTGGASADSSSAAKAAAP
                     LPASPSAASLDAAPPASETGGFDGRKAYEHVAKLVSFGPHPPASDAIHRVQDYIRSQL
                     KMFGCATDEDDFNAQTPIGTLAMKNIVAKIPGNGQGIILLLTHYDTLRLDNFVGADDS
                     GSSTGLMLEMARILCSQPKRANAVWIAFLDGEEAQVVTGGQAQWSDADSVYGSRELAA
                     RLAVSGKLKTIKAVILADMIGQRNLEIPRESSSTRWLTDLVWHTAKRLGYKDIFVSRE
                     EAVSDDHGPFLSRNVPATDIIDYSGYVSPGYWHTPQDTLDKISARSLAVVGHVILESV
                     AELQKR"
     gene            5822..6565
                     /locus_tag="VN788_13060"
     CDS             5822..6565
                     /locus_tag="VN788_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HXU21505.1"
                     /translation="MALQEYRKKRNFKISPEPPGTGKAGTPHARDGIYVIQKHRASHL
                     HYDFRLESNGTLLSWAIPKGPSLDPSVKRLAMQVEDHPIEYAEFEGVIPPGEYGGGTV
                     MVWDKGTWSPEVPDVSAALEKGDLKFAINGEKLAGSWVLVRTHGYGRSSKPSWLLIKH
                     RDRFASSTRDIIDEMPRSAVSNRLLAEIARAEGGDVAVAATGDPPMNEDKSHHAAKNP
                     VRSQLSKVAPSSVHDEKIDDKKRRKHLPK"
     gene            complement(6719..10801)
                     /locus_tag="VN788_13065"
     CDS             complement(6719..10801)
                     /locus_tag="VN788_13065"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU21506.1"
                     /translation="MRPRIAALKFACAILICFVAPFISISPALAQSASTGALQGTVID
                     PSGAVISGATITATNLATGQSRSATTDSNGSYRFSLLPPGNYDVKFSAPGFKTAEVPS
                     VTVNVTETPVLNRSLEIGAQTQEVTVESTAETIQTQNATIGGLVSGQTVTELPLSTRN
                     YTQVIDLSPGVVANVATATAVGNGTMDINVNGSGSDQNTYMMDGVITTNYGSGGAAQS
                     GSYAGIPIPNPDSIQEFKVQTSQYDAAYGQNPGANVNVVTKSGTNQFHGAIWEFNRNN
                     FFNANDFFYKRSEANEGLPNKPPEVKQNQYGGTFGGPIRKDKIFFFGSYQGTRQLNGI
                     GSNGFATSLTQVGLLPLNEPGQPFATARGDNSGTIPLDFTGAPCNYTTYRQYLGCAFA
                     GETDFASGLGQAGHFVNSDGSNISNTAIALLRQTEKIPQVKGGFNNGYYLPSLRYNSA
                     GLPFCIANNFSCATPTTISQATIANEDQYMINSDYVLNSKNTLSEKYFFSKDPQIQSF
                     SCIVTGCDPGAPENAHYGSQSAVVRLTTVVSSNFVNDAFVSAQRLFLNVADGLTVQSC
                     DGDGITPLNITPAVNDGAPCPLSSAAAGNREDSLIPVIGSLGIPAGSQWGSWTEGGNF
                     FSATRSIQQSFLAGDQISWNHGKHSFRAGVNTQRIQWNWAQPNRERGWVVVGDMADIL
                     LSNSGLASDEANGVAGTPVPANYLINLSYRLIPNGSGNPHHWRISEYSTFAEDDIKVT
                     RRLTLNLGVRWEYDGWPSDAHGVFTNFSAQAAGLVNTGTALLDNSVGTLAGYIVQKNY
                     NRPIYGNLTGEFGSTGILVNSNKTLLNGSPLDNFSPRIGLAWQAMDKLVIRAGYGMFF
                     DRVYGNLVGDNIVGNMPPYATGIGANPGQTLQDPFCPVCPQFLGFIPRTLATGPAIPN
                     AASGNGLSNITDAFGGNASGLLNPGDDPAMRTPKIQQYNLDVQYEFAHGWIADIGYVG
                     SHGIHLYDWNRDPNIAYLVDCGPASATCNPPTDQVNINLERPASSFPINDNGNNRVLE
                     NTSSNYLGRVAYLGVNPGNLQQVETDGNHLYNSLQLQLKHQFSHGLTLQASYTWSKLI
                     TDINASAAGAGIATPGNVLSGSASSNDPLNRRQQYGLAAFNRPQRFVVSYAYQIPYKA
                     EGWKEKALGGWGISGVTTIQDGLPFSITNGANGNQATLLYGASLPATGPVDRAELSTP
                     VDCNAVTGNCKSGVSLRAPGDMLKLVENGKSIINAAAFTAAPLFGGTPSALAGPYTGT
                     CTGANPQFLGCGTGFGNSGVGIMSCCTQLNFDAAIIKNTTVGGLRENATLQFRAEFFN
                     LWNHGQFNEPGNAFGGANFGVITSSAVPGRILQFGLKYIF"
     gene            11060..11491
                     /locus_tag="VN788_13070"
     CDS             11060..11491
                     /locus_tag="VN788_13070"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HXU21507.1"
                     /translation="MVRASILPRRSNRDAFVSRSEFSRGKKCSIKALHRIPALIAFAA
                     VLALPLRLTFAQNSPSVRAVDPTSGKVNDSISVEGQNLGKGVVSAVYLSDEKSDYKAI
                     IVEQASDKIVLKIPQVKPGDYNVSVQTGASIYIQPVRLTVQ"
     gene            11616..13493
                     /locus_tag="VN788_13075"
     CDS             11616..13493
                     /locus_tag="VN788_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HXU21508.1"
                     /translation="MTETPALARSNSEAPLRPVRLRTSRASMERRPDGAIIVRPDEPL
                     GPYPRVLTERLIHWASVVPDRALAAKRQANGEWRYLTYAEALQKVRAIGQAFLDRGLS
                     AVKPVAILSENDLEHLLLMFAGQHVGVPTASIAPPYSLLSTDFAKLKHVMSTITPGLV
                     FVSNAEPYRRALAAAVSPEVEVVFARGPSPDCRATSFDDLAARVPTAAVERAHADVAS
                     DDIAKFLFSSGSTGVPKAVINTHRMICSNQQMILQGFPFMGDQPPVVLDWMPWNHTAG
                     GNHNTGMTLYNGGTLYIDDGNATFKIATTVANLREIAPTVYFNVPRGYEELLRYFRQD
                     ARLRENFFSRLNFMFYAGAGLAQSVWDGYRTLMSETRGERVLMASGLGATETAPMALQ
                     CTWDSEQSGAIGIPMPGVEAKLVAAGAKLEIRVKGPNVTPGYWKNEDLTRAAFDEEGY
                     YKFGDAVRFVDPNDVNKGFMYDGRLKEDFKLASGTWVSVGPLRTKTIHAFAPYVTDVV
                     IAGLNRNFLSMLIFPNLEACRSLSRELGDAATPAEICRHSSVTGKFTELLENFATQST
                     GNSNRIARAVLLEDPPSLDAGEITDKASLNQRAILDRRAATVEEMYSRHPPPRVLRLR
                     D"
     gene            13615..14319
                     /locus_tag="VN788_13080"
     CDS             13615..14319
                     /locus_tag="VN788_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21509.1"
                     /translation="MYRHCAYRLTLMLGLILSLAAVLSAQSTPNATPNASNKTLVVNG
                     KAVPAGVRQINGRAYIDVETLAQATNGVVKMESNRVVLTIPGPNSTVSPGAPPSNSQE
                     LSRSFAVGAIGEVSEMREWRGAIGAMVTYGLAISGSWAQDYRNRVQTGLTQLASVAST
                     DGDRNALQLLTSEFDNLAEWSNEIFTARQNLNGAKTVDPNALSNDPQLAKITNCSSSL
                     EGMLISGTFSDDGGCH"
     gene            complement(14316..14822)
                     /locus_tag="VN788_13085"
     CDS             complement(14316..14822)
                     /locus_tag="VN788_13085"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU21510.1"
                     /translation="MNRFIPKSLAAAVVGAMLIAGAALCARAQVSVQDEVLDLQKKFQ
                     AATVSGDTAAVASLMADDVIFIHGNAAAQSKTEFLAGIKNGQLAFSAYDLSDPKVVMF
                     KGGAIVTGIVDISFRPRPNAPGAPRTLHMRGSSVWLHGSTGWKLILDQDTPIQGPPRE
                     VPLAPRLD"
     gene            complement(14915..16129)
                     /locus_tag="VN788_13090"
     CDS             complement(14915..16129)
                     /locus_tag="VN788_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009517282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HXU21511.1"
                     /translation="MAEGTQQKRSTDGSAYGRSPQHPDFELTQVGPGTPCGELLRRYW
                     QPVGVSSKVTDRPQYVRILGEDLIIFRDKKGRPGLLYARCMHRGTTLFYGKVSEEGIA
                     CCYHGWLFDVEGRCLRQPCEPDGGRNLDVARQPWYPVEERYGLAFAYMGPLEKKPVLP
                     RYDVLENPGPDEELRASIGAFGATGDNSLEVAPYSWLHMNDNVMDPFHVQVLHSTFST
                     VQFVPQFALMPKVEFFTTENGVCYSAKRKLDDGREYDRISSFIMPNVMCVPNPINIEE
                     VRPNRMSWSLAIDDSHFTMATASRVPKSTPREFSGVRFNGKLWGEMTIEERQATPGDY
                     EAQAGQGPISLHSEEHLASSDRGIIMQRRLLKSQIKIVAEGGDPVGVVFDPDKALVKV
                     RSGNFYNAPQPA"
     gene            complement(16276..17049)
                     /locus_tag="VN788_13095"
     CDS             complement(16276..17049)
                     /locus_tag="VN788_13095"
                     /inference="COORDINATES: protein motif:HMM:NF026037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21512.1"
                     /translation="MKLRSAIVALCATLFVAIIGCSKKSSESSEPPPPTAVVDASTSG
                     SITGTVKLEGEPPVFRPIDMSAEPTCVKANPSPVIPPIVVTGRNHALANVVVYIKSGL
                     GRYRFDTPKETAVLDQKGCMYVPRVLAVMVNQPIAFKNTDPTTHNIHPMPRDNHAWNR
                     SLDAGEEPYVTTFSHPELAIPVACNIHPWMRAFLFVFADPYFAVTPKTGDFELKNVPP
                     GTYTIEAWQERFGTQDTSVTIGPNESKSISFTFKSAKSR"
     gene            complement(17120..18091)
                     /locus_tag="VN788_13100"
     CDS             complement(17120..18091)
                     /locus_tag="VN788_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HXU21513.1"
                     /translation="MAEAEVGDDVYAEDPTVNRLQSRAAEIFGREAALYFPSGSMANL
                     TAIKTWTRPGMEVICEAQAHIYLYEMAGVAAIAGCMPNTVPAPGGTLTWDLIEPLIRP
                     HIYYRAQTALIELENTHNMHGGTVYPPEIADDICDHAHALNLPVHLDGARVFNASVAL
                     GRGVAEITRKFDSVMFCLSKGLGAPVGSMLVGSRDFIDKARVTRKLLGGGMRQAGVLA
                     AAGLVALEETPKLLHRDHENARHLAEGLARIEGITLDPRKVVTNIVIFDVRATRRTAA
                     EICAALAKENILCGATGPFAIRMVTHYDVDRAGIDRALSAIAAVVRS"
     gene            complement(18242..19027)
                     /gene="ubiE"
                     /locus_tag="VN788_13105"
     CDS             complement(18242..19027)
                     /gene="ubiE"
                     /locus_tag="VN788_13105"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052624 - 2-phytyl-1,4-naphthoquinone
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0042372 - phylloquinone biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HXU21514.1"
                     /translation="MIAAQKQTSLPGTRPEGTRDEREAAARVRDMFTRIAPGYDFLNH
                     LLSFSLDKVWRRRTAKRFVNILQHPEARVLDVCCGTGDLAFALERIRSRTTAAARREP
                     IVGSDFVEAMLVRAGEKARQANSSAIFAAGDALRLPFSDASFDLVTTAFGFRNLANYE
                     DGLREFARVLKPRGELGVLEFTEPGAGPLAGIFRFYFRHILPRIGAAVSGNSEAYAYL
                     PNSVRKFPSSGELASLMQSCGFTAVRIASWNFGSVILHSARRS"
     gene            complement(19176..>19572)
                     /locus_tag="VN788_13110"
     CDS             complement(19176..>19572)
                     /locus_tag="VN788_13110"
                     /inference="COORDINATES: protein motif:HMM:NF013887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HXU21515.1"
                     /translation="LRQILNFGHTLGHALEAATKYRRFLHGEAIGWGMIAATMMALGV
                     GRLGDSDATRIIRLVASIGSLPALGNIRASQLRPILAGDKKARGGRVLWVLPRRIGKT
                     EWGAEVAWPVVARAFDGLAGIVERAALDS"
BASE COUNT         4162 a         5601 c         5616 g         4193 t
ORIGIN      
        1 gcagcttccg cgctgcgtga aaacgttgac gcgaaatcgc gaaatcttcg gcagactgta
       61 ggaaatgtcg caggatcctt cctgcttgag cttgtcaacg gcatgcgagt tgcgcccaat
      121 cagatcggcc gcaatacgcg ctgtatcctc cgatcccagg cgcccgacgc cgggaatatt
      181 caattgcacc aattggccat tggcttctat ttgtggagca cggccgggtg aaaagatgag
      241 gtcgctgatc tttggcgagg cgcgaagcat cgctgcgatg attgatggag tcggtaacgg
      301 ttgagccggg gtggctttct cggctttcgg gcttggctgc tgagtttcgt ccataaggac
      361 ctgctttcca cctggtagcc cgagagagat gacctaccgg cctcttcgct gcgcctataa
      421 gtatggccgc aatccgaccc ctggctggtt tctaaagcag ttgctgttgt caaacgatta
      481 ggtggccgtt tggacaggac gtaacagtcg gcgcttggcc aggcatctca tggcggattc
      541 ctttgacgca aacaaatttg cgccggcgag tccgagttgg attattatcc ctttaagatg
      601 gtgggaaggg cgattatcgg cagttcagca gtgcatcggg cccgatatac ccgcaccgcc
      661 cggctgttgc gcctcttccc tgtgtgtgcc gccagcctgt gcctgctcgc cgtttcccct
      721 gagctttacg cacaaaaatc gctgccatct catccaattg gcaacccgcc aactacgtct
      781 cgcaacggcc cgaactttgg accggggtct attgtgccag gcacggctgc ggaccccttc
      841 gatgatctgt atggaacgcc gccaatggcc gtgatgccct ccccttccga cggcttgatc
      901 gaatcagaag gatgcaattc gtggacggaa tcgggagtgc gcagccccac ggtgagcgtc
      961 gcaagactgg cggtgccggg aaaagccagc ggcgaatacc aaaaaggatg cggcgcatac
     1021 aaggacaagc gtttcgatca ggccgagcgg cattttcgca aggcaatcga tatctatccg
     1081 agctatccgg cagcatgggt ggtgctaggg caggcgctag acgcgcagca caagcgggac
     1141 gatgcaaggc aggcctgctc gcaggccatc aagatcgatc ctacttacgt ggcgccttat
     1201 ctttgcctgg cggagttttc agcgaccgag gaccactgga agcaggttgc aaacttgtcg
     1261 aagcgcgcac tggcgatcga cccggtgaac aatccttatt cgttgtatta cacggccgat
     1321 gccgcttttc acctcaataa cctggccgag gcggaggcaa aggcgcttgc tgcagtcgcg
     1381 ctcgacccat ggcatcatct cccgcaacta cacctcctgt tggcccagat ttatgcggcc
     1441 aaaggggatc tcgatgcgga ggccgcgcag ttgcgaaatt atttgaaggt ggcgcctaac
     1501 tcgggggatg ccgccggagc tcgcgacaca ctcgcgcaac tccagtcacg acccgcagcc
     1561 gcgaaagcgg atccccagcg ctagggttga gtgctggact ggcgttccgc aacaattttc
     1621 ctggcattcg cgtaaagcgc cttggcctgg ggaatcgact cttcggcctt aacgagctgc
     1681 ggatcattat ccaactcgac tttgtagcct tcgttcaatc caaatagagt ggtaaatacc
     1741 tcgcgcttaa tttcccattt gagccacgga agattttgct ctatctgctg atcggtgact
     1801 cggatgttct gacccgctag atattttttg aactgggcaa ttaccgcgtc atcgacaacg
     1861 aagtccttgg tcacattcgg cttctggcca agataaaatc gcgcgaagtc tcccacgcct
     1921 tccggaagag gatagaagat acccctccgc tcaagtagat cctcaaaatc atccggcttg
     1981 ggagcggcga caaccacgtc cggagtaatt ccaccctgtc cgtatacttg gcgaccggta
     2041 tcggtcaact ttacttccgg ggccttcgga cgctggggat tatagtggta gtcgtagagg
     2101 gtcacgttct tgtaattgcg ctggatcaac cggccgctgg gcgtgtagta acgagcggtc
     2161 gttagcagca gcgcagtatt ctcgctcagg ggaaactgcg tctgcaccaa accctttccg
     2221 aaggtggtct cgccaacaat tagtccgcgg tcgtggtcct gaatggcacc ggagacaatt
     2281 tctgatgccg aagcgctctg cccgtttacc agcacgatca acggaacttc gacgccctga
     2341 tttccccgtg ccgaatagta ggggcgctca ggagaagagc gaccgcggtg agaaacaacg
     2401 agttcgttct tgtccagaaa catatccgcc atgccgacgg cctgattcaa caggccaccc
     2461 ccattgcctc gtaagtcgat aatcagccca tccagtttgg atacatccag atgtttcagg
     2521 gcgtcggaaa ggtcgctatc agtcgtctcg ttgaaggtgg agacgcgcac gtatccaatc
     2581 ccgggtttca ccatttcgta gaactgcacg ccaggcctcg ggatttcgtc gcggacgatt
     2641 gtgacttcga tgggtttctc ccagccctcg cgtcccagcg agatatgaac cggggtcccc
     2701 ttggcgcctt tgagcatctc ggctacttca gttgtggtca gcccagtgca ggtttttcca
     2761 tcgactttga ggatgatgtc accggggcgg attccggcct tataggcggg agagttgacg
     2821 aaaggcgact gcacgactgt gttgttgtcc cgctgcgcta cgaccatgcc gacgccgtag
     2881 tagcggcctt cctgctcttc gcgaaatagc gcgtactgct tgggatcgaa gaaatttgag
     2941 tgagggtcaa gaacatgcaa catccccggg atggcgccat catagatcgc tttatcggtg
     3001 tccacgggat cggcatagtt ccgttgcacg acggagagaa cgtgggtgaa gctcttcaca
     3061 gaatcttggg cgtcatcgga accagcggcc gtggcgcgcg cctgggggcc atagacgaag
     3121 ccgccgagca gcgccgatac cgccagtacg accaccagca aggcaatgcc tcgcgcttgg
     3181 gatcccccgt tttgcgggcc ttggcccatg ccgctcgatg ctgaagatgg tgagtgttcc
     3241 atgcagcctc ttttcgactt tgataccgga agtatagcac agcgctctgg gctgtttgac
     3301 aggtccacgg ccctcttcta tgatgcaaaa agagcgcaaa tgggcgctgg ttcttcggct
     3361 tccaatgctg agcatccccc atctgattat aatctttgta gtcgcactgg tgattttcgg
     3421 gcccgaaaag ctacccgagc tggcgcgaaa cctcggaaaa gtgatggcag agttccggcg
     3481 gcatacgagc gacctccgca cgactttcga agcgcatctg cgggatatcg aacgcgaagg
     3541 cgaatcgcgt cgcatcgccg cggtgcctgc gccggtggct cagcctccgg ctgcggaacc
     3601 acccgcgcct gcttcacccg gcaccgtacc ctccaaaccg ccgaatgccg ccgccatcgc
     3661 ggccccgagc gacgatccct tttccgttga tgttcccgat ccggacctac gccggaagga
     3721 tcccggggac gacccctacc tttccggcac cgagtttgat cctggcggcc cgccggaaga
     3781 aacgcaagcc accgagcccg agaaaaattc tgaacgagta gaaaatggcg gcgtccgccc
     3841 ctcctgaact cacaccgcgc cagcatgccg aggaagaatc cggcgctgca atgtcctttt
     3901 tcgaccattt ggtcgagttg cgcaaacgca tcatcagcgc gctgatcgcc gtcggaatcg
     3961 gaatggcaat tggcctgacg gtcgccaggc gatttattaa ctttatcagc cagcccatgt
     4021 gggtcgcgtt gcgcgcgaat cacatcgatc ctaatttgta ttacacgcac cctgcgggtt
     4081 acgtcagcct gtacatcagt atcggccttt acttgggaat cgcgcttgcg atgccctggg
     4141 tgctgtatca agtgtggttg ttcgtggctc cgggactcta caagcacgaa aggcgcgcgg
     4201 tcgccagctt catcggatcg tcgatgtttc tctttctttg cggaatcgcg tttggatatt
     4261 tcgtaatgct tccacaggtt ttgacctacg tggtggcatt cgccaatgga ggctccgtcg
     4321 gaggctccat caaaccgctg atcgatatca acgaatattt cgatctgatt ctggtggtgc
     4381 tcgtgggtct gggagtgatt ttcgaattgc ctgtggttgt attcattctg tcgctcttcg
     4441 gaatcgtcac accgaaattt ctcatgcgga atttccgata tgcgctgctg ctcatcacgg
     4501 tggcggcagc gattgtgaca cccacgcctg atgcgaccac tatgctaatt tttatggcgc
     4561 cgatggtcgt gttgtatttt atcggcgtgc tcgtttcata cgcagttttg cgaaacaagc
     4621 gcgccaagga actggcggaa ggacaggcgc cgccaggatg acgcgcgttc tgcgattgcg
     4681 ctggggcggg gttgccgcgc tcgcgttgct gctatcggcg tcgtgcggac atggcggcac
     4741 gggcggcgcg tcggcagact cttcttccgc ggcgaaagcg gccgcgcctt tgcccgcgtc
     4801 gccaagcgcg gcgtcattgg acgctgcgcc acctgcgtct gaaacgggag gcttcgatgg
     4861 ccgaaaggcc tatgagcacg tggccaagct cgttagcttt ggaccgcatc ctcccgcctc
     4921 tgatgcgatt caccgcgtgc aggactacat tcgttcgcaa ttgaaaatgt ttggttgtgc
     4981 gaccgacgag gacgatttca acgcccagac gccgatcggc actctggcga tgaaaaatat
     5041 cgtggccaag attcccggca acgggcaagg aatcattttg ctgctgaccc actacgacac
     5101 gcttcgcctg gataattttg ttggcgcgga cgatagcggg tcttcgaccg ggttgatgct
     5161 cgaaatggcg cgcattttgt gcagccagcc caagcgcgct aatgccgtgt ggattgcgtt
     5221 tctcgacggc gaagaagcgc aggtggtcac gggcggtcaa gctcagtgga gcgacgcgga
     5281 tagcgtctac ggcagccgcg aattggctgc gcgcctcgcc gtctccggga agctgaagac
     5341 aattaaagct gtaatacttg ccgacatgat cggtcagagg aacttggaaa ttcctcgcga
     5401 atcaagttcc acgcgttggc tcacggatct tgtgtggcac acggccaagc ggctcggcta
     5461 caaagacatt ttcgtttcgc gtgaggaggc cgtctcggat gatcacgggc catttctcag
     5521 ccggaatgtt cctgcgaccg acatcatcga ctacagtgga tatgtgagtc ccggttattg
     5581 gcacaccccg caagacacac tggacaaaat cagcgcgcgc agcctcgctg tcgtaggcca
     5641 cgtcattctc gaatccgtcg cagaacttca aaagcgctaa tttcccgaaa agctcagtta
     5701 gccatcgagg gcggtgatcg tcccaaatgg cggatacact acaggctttg cgggatggtc
     5761 gcggggacat caacttccgt atgttggttg ccgaactgca acaatgtacc cggagtgtcc
     5821 gatggccctg caagaatatc gaaaaaagcg taactttaaa atttctccgg agccgcccgg
     5881 gacaggcaag gctggcacgc cgcacgcccg cgacggcatc tatgtaattc aaaaacatcg
     5941 tgcctcacat cttcactatg actttcgact ggaatcaaat ggcacactgc tctcctgggc
     6001 catacctaag gggccatcgc ttgatccatc tgtaaagcgc ctcgctatgc aggtcgaaga
     6061 tcatccaatc gagtacgccg agttcgaggg agtaatcccg ccaggcgaat atggcggcgg
     6121 gacggtaatg gtttgggata aaggcacgtg gtcgcctgag gtgcccgatg tttcagccgc
     6181 actggagaaa ggggacctta aattcgcgat caacggagag aaactggcgg gctcatgggt
     6241 gctcgttcga acgcacggct acggccgtag ctcaaagccg agctggctgc tcataaaaca
     6301 ccgagatcga ttcgcttcat caacccgaga cataattgac gaaatgccgc gatcggcagt
     6361 tagcaatcga cttttggccg aaatcgctcg tgccgaagga ggcgatgtcg cagtcgcagc
     6421 gaccggcgat cccccgatga acgaagacaa gagccatcat gccgccaaaa acccagtccg
     6481 ttcgcagctc tcgaaggtag cgccatcatc agtgcatgat gaaaaaatcg acgacaaaaa
     6541 acgaagaaag catctaccta aatagagccc tggtgctgcg tcgatttgcg cgagaggcat
     6601 ccgccggctc ggatcggtaa agaatccata tccctactgg cgcatgttcg attctcggtt
     6661 gatatctgcc gaggccctgc ttccgccgct aactttcgct ttggaggtag acaatcagct
     6721 agaagatgta tttcaagccg aattgcagga tgcgccccgg aaccgcgctc gacgtgatca
     6781 ctccaaagtt ggctccgccg aaggcgttgc cgggttcatt gaactgtccg tgattccaga
     6841 gattgaagaa ttccgcgcgg aactgaagcg tggcgttttc tcgcaatccc ccgacggtcg
     6901 tgttcttgat gatcgcagca tcaaaattga gctgcgtgca gcaactcatt attccgacgc
     6961 cagagttgcc gaaacccgtg ccgcagccca ggaattgggg attggctccc gtacaggtac
     7021 ccgtgtaggg gccggcaagc gcgctcggag tcccgccaaa caggggtgct gccgtgaacg
     7081 cggctgcgtt gataatcgat ttaccgtttt ctaccagttt gagcatgtcg ccgggagcac
     7141 ggaggctcac ccccgacttg caattgccgg tgacagcgtt gcaatcgacc ggggtagaaa
     7201 gttcggctcg gtcgactggg ccggtggcag ggagtgaggc accgtacaaa agggtcgcct
     7261 ggttgccatt ggctccgttc gtaatgctga aaggtaggcc gtcttgaatc gtggtcacac
     7321 cggaaatgcc ccacccgccc aaagcctttt ctttccaccc ttcggccttg taaggaatct
     7381 ggtaggcgta gctgaccaca aagcgctggg gccgattgaa cgcggccagc ccgtattgct
     7441 gccgacgatt caagggatcg ttggaggacg cgctgccgga aagtacgttg ccgggcgtgg
     7501 cgatgccagc tccagcggcg ctggcgttga tgtcggtaat gagtttgctc caagtgtagg
     7561 aagcctgcag ggtcaggccg tgcgagaact gatgctttag ttggagttgc aagctgttgt
     7621 aaaggtgatt gccgtccgtt tcaacctgct gcaggtttcc gggattcaca cccaagtaag
     7681 ctacgcggcc aaggtagttg ctagaagtgt tctcgagaac tcggttgttg ccgttatcgt
     7741 ttattgggaa cgagcttgcc ggtcgctcca gatttatgtt gacctggtcc gttggcggat
     7801 tgcaggtggc ggatgccggg ccgcagtcca caagataggc gatgttggga tcgcggttcc
     7861 aatcgtagag gtggatgcca tgcgatccca cgtatccgat gtcggcgatc cagccatgtg
     7921 caaattcgta ctgaacatcc agattgtatt gctggatttt gggggtgcgc attgccgggt
     7981 catcgccagg gttgagaagg cccgaagcat tgcctccgaa tgcgtcggtt atgttcgaga
     8041 gcccattccc agaagctgca ttgggaatcg ccggacccgt agccagggtc cgcggtatga
     8101 agcccaggaa ctgcgggcat acagggcaaa acggatcctg aagtgtctga cccgggttcg
     8161 caccgatgcc ggtggcgtag ggaggcatgt tacccacgat gttgtccccc acaagatttc
     8221 cgtaaacgcg gtcgaagaac atgccgtaac cggcgcggat aacgagctta tccatggcct
     8281 gccacgccaa accgatgcga ggcgagaagt tgtcaagcgg agagccgttc aacaaggtct
     8341 tgttactgtt gaccaaaatg cctgttgacc cgaactcacc ggtaagattt ccgtagatag
     8401 gcctgttgta attcttttgg acgatgtatc cggcgagagt acccaccgag ttgtcgagca
     8461 gcgccgtgcc cgtattcacc aggccggcag cctgggctga gaagttcgtg aacacgccat
     8521 gggcatcgct gggccagcca tcgtattccc agcggacacc caggttcagg gtgagacgac
     8581 gtgtgacctt gatgtcgtcc tccgcgaacg tcgagtactc gctgatgcgc cagtggtggg
     8641 ggtttccaga gccattcggt atcaggcgat aggaaaggtt gatgaggtaa ttggctggaa
     8701 cgggggtgcc tgcgacaccg ttagcctcgt ccgacgcaag gccgctgttg ctcaacagga
     8761 tgtcggccat atctcctacc acgacccaac ccctttctct gttgggctgc gcccaattcc
     8821 actggatgcg ctgcgtattg acgcccgctc gaaacgagtg cttgccatga ttccaggaaa
     8881 tctggtcacc ggccaagaag ctctgctgga tgctgcgggt cgcggagaag aaatttcctc
     8941 cctccgtcca cgaaccccat tgcgaacctg cgggaatccc caagctgcct atgaccggga
     9001 tcaaggagtc ctcgcgattg ccagcggcag cacttgagag ggggcaaggc gcgccgtcgt
     9061 tgaccgctgg agtgatgtta agaggggtaa tgccgtcacc atcgcaggac tggaccgtaa
     9121 gaccgtccgc gacattgagg aacagccgct gcgctgagac gaaggcatca ttcacgaaat
     9181 tgctgctgac caccgtcgtg agcctgacta ccgccgactg cgaaccgtag tgggcatttt
     9241 caggggcgcc cgggtcgcaa ccggtgacga tgcagctgaa actctgtatc tgcgggtctt
     9301 tggagaagaa gtacttttcc gagagtgtgt tcttgctatt gaggacgtaa tccgagttaa
     9361 tcatgtactg gtcttcgttg gcgatcgtgg cttgcgaaat cgttgtaggt gtagcgcacg
     9421 agaagttgtt cgcgatgcag aaaggaaggc ccgccgagtt ataacgcaaa ctggggaggt
     9481 aatagccatt gttgaagcct cccttgacct ggggaatctt ctccgtctgg cgcaagagag
     9541 cgatagcggt gttactgatg ttggatccgt cggaattgac gaagtggccc gcctggccta
     9601 agccgctagc gaagtccgtc tcacccgcaa atgcacaccc caaatattgc cggtaggtgg
     9661 tgtagttgca cggggcaccc gtaaaatcga gtggaatggt acccgagttg tcgcctcgcg
     9721 cagtggcaaa cggctgaccg ggctcgttga gagggagcag gccgacctgc gtgagggagg
     9781 tggcgaaacc attgcttcca attccattga gttgccgcgt gccctggtag gagccaaaga
     9841 agaaaatctt gtctttcctg atggggccgc cgaaggtccc gccatactgg ttctgcttca
     9901 cttccggcgg tttgttcggc agaccttcat ttgcctccga tcgcttgtag aagaaatcgt
     9961 tggcgttgaa aaagttgttg cggttgaatt cccagatcgc tccgtgaaac tggtttgttc
    10021 cgcttttggt gacgacgttt acgtttgcgc cggggttctg tccgtaggcc gcgtcatact
    10081 gagaggtttg gaccttgaat tcctgaattg agtccggatt gggaatcggg atcccggcat
    10141 aactgcccga ctgcgccgct ccgccactgc cgtagttcgt ggtgatgacc ccgtccatca
    10201 tgtacgtgtt ctggtcggaa ccggagccgt tcacgttgat gtccatggtg ccgttgccaa
    10261 cggcagtagc agtggcaacg tttgcgacga caccgggcga aagatcaatc acctgcgtgt
    10321 agtttcgcgt gctcaggggc agttccgtga cggtttgccc ggagaccaat cctcctatcg
    10381 ttgcgttctg cgtctggatg gtttcggcgg tcgattccac ggtgacttcc tgcgtctgcg
    10441 cgccgatctc gagggagcga ttcagcacag gtgtttccgt gacgttcacg gtgaccgaag
    10501 gaacttcagc ggttttgaaa ccaggggcgg aaaacttcac gtcatagtta cccggcggca
    10561 gaagcgagaa tctgtaggag ccgttgctgt ccgtcgtcgc gctgcgcgac tggccggtgg
    10621 cgagattcgt ggccgttata gttgcgcctg aaataaccgc tccggaagga tcaatcaccg
    10681 ttccttggag cgctccggtg gaagcacttt gtgccaacgc cggagaaatg gaaatgaacg
    10741 gcgctacaaa acaaatcaaa atcgcacacg cgaacttcaa cgcagcaatt cgtggacgca
    10801 ttgccccccc cagaatgtgt tgtacggatg tgatttcgcc ccccacgggc gactatcgtg
    10861 ggcgaatgtt tactaccggg cggctgccga tgtcaagagg aaaggggacg gaatatcgcc
    10921 gctagagggc aatgtcaagc tgaaagctgc cgaccccggg cctgcatcta gaatttgaca
    10981 acgcgggatt ccgggaatat cttctgcgca acaggaggtt gaaatccgac ttcgaaatta
    11041 tctctttacg aggtgaccca tggtcagagc ttcgatcctg ccccgtcgtt cgaaccgtga
    11101 tgcctttgtt tctcgctccg aattttcccg ggggaaaaag tgctcgatca aggccttgca
    11161 ccggataccg gcgctgatcg cctttgccgc tgtgctggcc ttgccattgc gtttgacatt
    11221 tgcacaaaat tcgccgagcg tcagggccgt cgacccaact tcgggcaagg tgaacgattc
    11281 aatcagcgtc gaggggcaga atctaggaaa aggcgtcgta tccgccgtct atctctccga
    11341 cgaaaagtcc gattacaagg ccatcatcgt cgaacaggct agcgacaaga ttgttctaaa
    11401 gattccacaa gtaaagcctg gtgattacaa cgtgtcggtt cagaccgggg catcaattta
    11461 tattcaaccg gtccgcctga ccgttcagta gcttacgttt cgtggcgtgg aaagtaacga
    11521 gagtttcttc agggtgcgac taaccggctc cctttgcgcc tcggggctcg cggtcgtata
    11581 ctcatcgccc aggcactcgc tgtttctgat actcgatgac cgaaacgcct gcgctcgcgc
    11641 gatcgaacag cgaggcgccc ctgcgcccag ttcgcttgcg aacttcccgt gcctccatgg
    11701 aacgccgacc ggatggcgcg atcatcgtgc gtccggatga gccgctcggc ccctaccctc
    11761 gcgtactcac cgaacgctta attcactggg ctagcgtggt tcccgatcgc gccctcgcgg
    11821 ccaagcgcca agcgaacggc gagtggcgtt acctcaccta cgccgaagca ttgcaaaagg
    11881 tccgcgccat tgggcaagca tttctcgatc gcggcctttc ggctgtgaaa cccgtcgcaa
    11941 ttctttcgga aaacgatctt gagcatttgc tgctgatgtt tgcaggacag catgtcggcg
    12001 tccccaccgc atccatcgcc ccgccgtatt ccctgctctc cacggatttc gccaagctga
    12061 aacatgtaat gagtacgatc acgccgggtc tcgtgttcgt ttcgaatgcg gagccatatc
    12121 gccgcgcgct tgcagccgcc gtcagtcccg aagtagaggt cgttttcgcg cgcgggccga
    12181 gtcccgattg ccgcgcgacc tcatttgatg atctcgccgc cagagttcct accgcagccg
    12241 tggaacgggc gcacgctgac gtcgcttcgg acgatatcgc gaaatttctc ttttcgtccg
    12301 ggtccacggg ggtgcccaag gccgtcatca acacccatcg gatgatctgc agcaatcagc
    12361 agatgattct ccagggtttt cctttcatgg gggaccagcc gccggtggtg ctggattgga
    12421 tgccgtggaa tcacaccgca ggcggcaatc acaataccgg gatgactctc tataacggcg
    12481 gcactctcta catcgacgat ggcaacgcca cattcaagat tgcaacgacc gtcgcaaatc
    12541 ttcgcgagat cgctcccact gtgtatttca acgtcccgcg cggctacgag gaattgctcc
    12601 gctatttccg ccaagacgct cgcctgcgcg aaaatttctt cagccgcctg aatttcatgt
    12661 tttatgcggg ggctggcttg gctcagtctg tttgggacgg ttatcgcacc ttgatgtctg
    12721 aaacacgcgg cgaacgcgtc ctcatggctt ccggcctcgg cgccacggag actgctccca
    12781 tggccctcca gtgcacgtgg gattcggaac agagtggcgc aatcggaatt cccatgcccg
    12841 gtgtggaggc caaacttgtg gcggccggcg cgaagctgga aattcgagtg aagggcccca
    12901 atgtcacgcc aggctattgg aaaaacgagg atcttacgcg cgctgcgttc gacgaggaag
    12961 gctactacaa atttggcgac gcggtccgct tcgtcgatcc gaacgacgtc aataaaggct
    13021 tcatgtacga tggccgccta aaggaggatt tcaagctagc ttcgggcacg tgggtcagcg
    13081 ttggcccgct gcgcaccaag accatacatg ctttcgcgcc atacgttacc gacgtagtca
    13141 tcgccgggct gaatcgcaat tttttgagca tgctcatttt cccgaatctc gaagcctgcc
    13201 gctcgctgtc gcgtgagttg ggcgatgctg ccacgcctgc cgaaatttgc cgccatagct
    13261 ccgtcaccgg aaaattcact gagctgctcg aaaacttcgc cacacaatcc accggcaact
    13321 cgaatcgcat cgcgcgagcc gtgctgctcg aagacccgcc gtcgctcgac gcgggtgaga
    13381 tcacggacaa agcttcccta aatcagcgcg ctatcctgga tcgccgcgcc gccacagtgg
    13441 aagaaatgta ctcacgccat ccgccgcccc gtgtcctgcg cctcagagac taacttcaac
    13501 agagtggcgg cgtgtaaact ccacaaatcg aaatgctgcg gaaaagttga tttgtgccta
    13561 tgcttggata ggtcgcaatg aatgcggtaa cgagcccggc ctagggagac caccatgtac
    13621 aggcattgcg cttatcgact taccttgatg ctcggactca ttctatcgct cgccgcggtg
    13681 ctctcagccc aaagcacacc gaacgctaca ccgaacgcgt ccaacaaaac gctcgtggta
    13741 aatggaaaag cggtgccagc aggagtccgc cagatcaatg gccgcgccta catcgatgtc
    13801 gagacgctcg ctcaagccac gaatggagtg gtgaagatgg agtcgaatcg agtggtactg
    13861 acgatacccg gtccaaactc gaccgtttct ccaggggcgc cgccttccaa ttcacaggaa
    13921 ctctcgagaa gtttcgcggt gggcgcgatc ggcgaagtat cggaaatgag agaatggcga
    13981 ggtgcaatcg gtgccatggt gacgtatgga ttggcgataa gcggatcgtg ggctcaggat
    14041 tatcgcaatc gagtacagac gggcctcacg cagctggcgt cggtcgcatc gaccgatgga
    14101 gacaggaatg cgctgcagtt actgaccagc gaattcgata atttggccga gtggtcgaat
    14161 gaaatattta cggcgcgcca aaacctgaat ggcgcgaaaa ccgtggatcc aaacgctctg
    14221 agcaacgacc cgcaactcgc gaaaattacg aattgcagca gttcgttgga aggcatgctc
    14281 atcagcggca catttagcga tgatggagga tgccactagt ctaagcgagg cgcgagaggt
    14341 acttcgcgag gcggtccctg gatcggcgta tcttgatcga ggatcagctt ccagcccgtc
    14401 gagccatgaa gccacacaga agatccgcgc atgtgcagag tgcgaggcgc cccgggagca
    14461 tttgggcgag gtctgaagct aatgtccacg atgccagtga cgatcgcacc gcccttaaac
    14521 atcaccacct tcgggtcgct caggtcgtag gctgaaaaag ctagctggcc atttttaatc
    14581 ccggcaagaa actcggtttt gctctgcgcc gccgcgttgc catgaatgaa aattacgtca
    14641 tcagccatca gggaggcgac agctgccgtg tcaccggaaa cagtggcggc ctgaaacttc
    14701 ttctgcaagt ccagcacttc gtcctgtacg gaaacctgcg cgcgagcaca cagagcagca
    14761 cccgcgatca gcattgcacc cacgacggcg gctgccagtg atttgggaat aaatcgattc
    14821 attgcaagtt ccattaatct ccccacccgt tcatcgaatt gaaagcagac ggattgtaca
    14881 tgcagcgcgt cgccacccga aaacgcacaa gtacttaggc cggttgcggc gcattataga
    14941 aattgccgga acggactttt accagggcct tatccggatc gaagacgact ccaacgggat
    15001 cgccgccttc ggccacaatc ttaatctgcg attttagcaa gcggcgctgc atgatgatgc
    15061 cgcggtcgct gctggcaaga tgttcctcag agtgcagaga aatcggccct tggccggctt
    15121 gcgcttcata atcgccggga gtggcctgcc gctcctcgat cgtcatctcg ccccacaact
    15181 tgccattgaa tcgcaccccg gaaaattcgc gcggcgtgga cttgggtacc cgcgacgcgg
    15241 tcgccatcgt gaaatgggag tcgtcgatcg ccagggacca actcatccta ttaggtcgca
    15301 cttcctcgat attgattgga ttaggcacgc acatcacatt cggcatgatg aacgacgaaa
    15361 tgcggtcgta ctccctgccg tcatccagct tccgcttggc cgagtagcaa acgccatttt
    15421 cggtcgtgaa aaattcaact ttaggcatca gcgcaaattg cggcacgaac tgcacggtgc
    15481 tgaatgtcga atgcagcacc tggacatgaa acggatccat cacgttgtca ttcatgtgta
    15541 gccagctata gggggcgact tctaggctgt tatcgccggt cgcgccgaag gcgccgatac
    15601 tggcgcgcag ttcctcatcc ggcccgggat tctccagcac atcgtagcga ggcagcaccg
    15661 gctttttctc cagcggcccc atataggcga aggccagccc gtagcgctcc tccacgggat
    15721 accagggctg gcgcgcaacg tccaggttac gaccgccatc gggctcgcac ggttgccgca
    15781 ggcagcggcc ttccacgtca aacagccagc cgtggtagca acacgcgatg ccctcttcag
    15841 aaacctttcc gtagaaaagc gttgtgccgc gatgcataca ccgggcatag agcaggccag
    15901 ggcgtccctt tttgtcgcgg aaaataatca gatcctcgcc gagaattcgg acgtattgcg
    15961 gccgatcggt aacctttgac gaaacaccga ccggctgcca gtagcgccgc aacagttcgc
    16021 cgcacggcgt gcccggtcct acttgcgtca actcaaagtc tggatgctgc ggagatctgc
    16081 cgtacgcgct gccatcggtc gagcgctttt gctgcgttcc ttcagccatg tgatgccccc
    16141 tccagatgcc atacaaaatt gcggtcgttc gaaaatgaaa gccaatcggc caacaatcgc
    16201 actaaacacc ttcggcgatt gggtgtcaat agggtgggaa cgcgttcaca gtgagcctgg
    16261 agcggccccg acgggtcagc gagatttggc ggatttaaac gtgaacgaaa tggatttcga
    16321 ttcgttcggg ccgatagtga cgctcgtatc ctgcgtgccg aatcgttcct gccacgcctc
    16381 aatcgtatat gtgcccggcg gaacattttt caattcgaag tcgcccgttt tcggcgtcac
    16441 ggcaaagtaa gggtccgcga acacgaacaa aaatgcgcgc atccatgggt gaatgttgca
    16501 tgcgacagga atcgcaagtt ccggatgcga aaaagtggtc acatacggtt cctcacccgc
    16561 atcgagcgac cggttccacg cgtgattgtc gcgcggcatg gggtgaatgt tgtgcgttgt
    16621 gggatccgtg ttcttgaatg cgatcggctg attcaccatc acggccagca ctcgcggaac
    16681 atacatgcag cctttctggt cgagcacggc cgtctctttc ggcgtatcga agcgatatct
    16741 gccgagacct gatttgatat acacgacgac attcgccagc gcatggttgc gccccgtcac
    16801 cacgatggga gggattaccg gagacggatt tgcctttacg caagtcggtt cggcactcat
    16861 atcgatggga cgaaaaactg gcggctcgcc ttccaatttt acggtgccag taatcgaccc
    16921 agaggtggac gcatcgacga cggcggtcgg cggcggtggc tccgaggatt cgctagattt
    16981 tttgctgcag ccgatgatgg cgacaaaaag cgtggcgcac agcgccacaa tagcggaccg
    17041 aagtttcaag aagacgattc ctttctcacc acttcatttt agcaacaagg cgagcgtgtc
    17101 acgctgcttt tgtggaattt caagagcgga ctacagcggc tattgcggag agggcgcgat
    17161 cgatgcccgc gcgatccacg tcatagtgcg tgaccatgcg aatggcgaac gggcccgtgg
    17221 ccccgcacag gatattttct ttcgccaggg ccgcgcagat ttctgctgcg gtccggcgag
    17281 tagcgcgaac gtcgaagatc acgatgttgg tgacaacctt tctcggatcg agcgtgatgc
    17341 cctcgatgcg cgcgaggcct tccgcaaggt ggcgggcatt ttcatggtcg cgatgcagca
    17401 gcttcggcgt ttcttccaac gccaccagtc ccgcagccgc cagcactccg gcctgtcgca
    17461 ttccgccccc cagcagcttc cgtgtcaccc gtgctttgtc gatgaaatcg cgtgagccga
    17521 ccaacatcga gccaacgggc gcgccgagtc ctttggaaag gcagaacatc accgaatcga
    17581 acttgcgcgt aatttccgcg acgccacgcc ccagcgcaac cgatgcgttg aaaacccgtg
    17641 cgccatcgag atgcaccgga agattaagcg cgtgggcgtg gtcgcaaatg tcgtccgcga
    17701 tttcgggcgg atacaccgtg cctccatgca tgttatgcgt attctcaagt tcgatgagcg
    17761 cggtttgcgc gcgatagtag atatgcggac gaatcagcgg ctcaatcaga tcccaggtta
    17821 gtgttccgcc gggcgccggc accgtattcg gcatgcaccc cgcgatagcc gccactcccg
    17881 ccatctcata aagatagata tgtgcctgcg cctcgcagat cacttccatg cccgggcgcg
    17941 tccaagtttt aatcgcggtc agattggcca tcgagccgga ggggaaataa agagccgcct
    18001 cgcgtccgaa aatctccgcc gcacgcgatt gcaagcggtt gaccgtgggg tcttcggcat
    18061 acacatcgtc gcccacttcc gcttcggcca tggcgcgccg catcgctgga gtcgggcgcg
    18121 ttacggtatc gctccgaaga tcgatcggta gttgagctgc cgtgtacaca gcgcgaccat
    18181 aatacctgag gtaacttggg agttgagagt ccaaaactag agcgagcgtc taaggctcgg
    18241 atcaggaacg acgcgcagag tggagtatta cgctaccgaa attccacgag gcgatgcgga
    18301 cagccgtgaa tccgcagctc tgcatcagcg aagcgagttc tcccgacgag ggaaatttcc
    18361 tcaccgaatt gggcaggtat gcgtaggctt cgctattgcc ggaaacggcc gctccgattc
    18421 gcggcaaaat gtgcctgaaa tagaaacgga aaatccctgc gagaggaccg gcgcccggct
    18481 cagtgaattc aagcacgccc agttcgccgc gcggtttcaa tacacgagcg aattcgcgca
    18541 gcccgtcctc atagttcgct agatttcgaa aaccgaacgc cgtggtaact aggtcaaaac
    18601 tcgcatctga aaaggggagc cgcaaagcgt cgccggcggc gaaaatcgca gaggaattag
    18661 cctgccgcgc cttttcgccg gcgcgcacga gcatagcttc cacgaaatcg ctgcccacaa
    18721 taggctcgcg tcgcgcggcg gcagtcgttc gcgaccggat gcgttccagt gcaaatgcca
    18781 gatcgccggt accgcagcaa acgtcgagga cacgtgcctc cgggtgctgc aaaatattta
    18841 cgaagcgttt cgcggtccgc cgtcgccaga ccttatcgag tgagaatgag agaagatgat
    18901 tgagaaaatc atatccaggc gcgatgcgcg tgaacatatc gcgcacgcgc gcggccgcct
    18961 ctcgctcgtc gcgcgttcct tcaggtcgcg tcccgggtag cgatgtctgt ttttgtgctg
    19021 caatcatcga aactcgccat ttccgctcaa aagtccacac tcaccgtgga cgacttttct
    19081 ttacaccaac aagtacttgc atcgacccag gcccttccgt gtaaaattta tgagtatcct
    19141 cgaagtacgc ccactttaat caggtgtcct gattcctacg aatccaacgc agcgcgctcc
    19201 acaattccag ccaatccatc gaatgcacgc gcgaccaccg gccaagccac ctcggcaccc
    19261 cattccgtct tcccgattct acgcggcaga acccacagca cgcgcccacc gcgtgctttc
    19321 ttgtcgcccg ccagaatcgg acgaagctgc gacgcgcgaa tattcccgag cgcaggcagc
    19381 gatccaatac tggcgaccaa ccgaattatg cgagtagcgt cggaatcgcc gaggcgtccc
    19441 actccgagag ccatcattgt cgcggcaatc atgccccacc cgattgcttc gccatgaaga
    19501 aaccgccggt attttgtggc agcttcgagc gcgtgcccca gcgtgtgacc gaaattcaga
    19561 atttgccgca ga
//
LOCUS       DATNDT010000402         3282 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_298116, whole genome shotgun sequence.
ACCESSION   DATNDT010000402 DATNDT010000000
VERSION     DATNDT010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_298116"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..1638
                     /locus_tag="VN788_13115"
     CDS             301..1638
                     /locus_tag="VN788_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21516.1"
                     /translation="MPRKPESVRDLPNPLNHHEQQELVLRVANSRAFVSSHTLRAFLL
                     YVAENAIAGKLDAIKEQYIGFHVLGRRPDYDPAEDNIVRVRARQLRQRLEEYFATEGQ
                     QEPVVITIPKGGYVPIFLERLPSPEIEPVAEVDVQPPTEPPQREQRHFRRFHVSVAVP
                     WLLATVAVLAACILWVKRDHKTLLTADPTTTDVPRGLWGQIFPTKGQELAVVTADAGF
                     ALWQDVTHHTLNLGDYLSRRYLEEGERPEMREVAARRTTSPADLSVSLRLAEIARVFG
                     GHVKVHFARSMDIHDFRNGNLVLVGSRRSNPWVELFERQMNFILDYNPNLRGPCFLNR
                     SPKPGESKTFSLRSPYTVQGSESEAIESYAVIALLPNSSGHGNVIILEGLSMEGTEAA
                     GEFVTNSERFGMLLRKIGLKNDAPIKPFEALLKLTAVAGGYADPELIAYRYPQ"
     gene            1714..2448
                     /locus_tag="VN788_13120"
     CDS             1714..2448
                     /locus_tag="VN788_13120"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HXU21517.1"
                     /translation="MPDNFLTPVTRTTLTSDIARKMVTHLIRGDWQPGERIPPERELC
                     LKLGVGRASLREALKALEIMGMIEIRLGDGTYVCNRSEFLSRPLLWAIASSSHAEAHE
                     LVEARKLIEIELAGLAAERATPRDTKQIKMHLDQMASGLTESAAFIQADIDFHLSIGQ
                     AAHNGILINALHLIRNLLQEWINSTLKIEGVAEKALEQHRSIFMAVSKRNRLAARSAM
                     ESHLDEMAAFLLEAQERDTRGSRTPE"
     gene            2491..3267
                     /locus_tag="VN788_13125"
     CDS             2491..3267
                     /locus_tag="VN788_13125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU21518.1"
                     /translation="MPLPMGFLDSKIILVTGGGSGIGRECALKYSREGASVCVADIDS
                     GAANRTCAELVGENLAVECDVSSGTAFREAVEETVRRLGGLDAVHNNAGISSPSKPLH
                     ETSEAEWDALLRVNVKSVYWSARFALPHLIERRGSILNTASMVGLIGQADHAAYVATK
                     GAMIALTKAMALDYAPHGVRVNAICPAGVWTPLLRRWAAEQQNPAEIEQYLDRIHALG
                     YCPEPDVVADAAAFLISDQARFLTGCILPVSGGAELGYRR"
BASE COUNT          739 a          890 c          972 g          681 t
ORIGIN      
        1 gcccaccatg tgggagccag ctaagggttg tccaagcaat ggcagggcag tgcccagaaa
       61 gctacaaatg aagaggaaat aaaacatgaa agctgaaatc aaaccttgcc ggaaccaagc
      121 gaatgaaaga tggttctacg atggcccgtg aaatcgtcgt gaataatctt tgaagtggaa
      181 caatattcgc cgggaatgat acattctggc gggctgtggc gattccgtga tattgttcca
      241 catgaaagag ctgccgggaa ggaacattta cagtaaactt aaccgtagcg tcacggggaa
      301 atgccacgta aaccagaaag tgttcgagat cttccgaacc cgctcaacca tcacgagcag
      361 caagagttgg tgctgcgggt cgcgaacagc cgcgcgtttg tcagttctca tacgttaagg
      421 gccttcctgc tctacgtggc tgaaaatgca atcgccggga aattggatgc gatcaaggaa
      481 cagtacatag gatttcacgt tcttggacgt agacccgatt acgatccggc ggaagataat
      541 atcgttcgcg tacgcgcgcg gcaattacgc cagcggctgg aagaatattt cgcgactgaa
      601 ggacaacagg agcccgtggt aattactatt cccaagggcg gttatgttcc gattttctta
      661 gaaaggttgc cttctccgga aattgagcct gtcgccgagg tggacgttca accgccgaca
      721 gagccgcccc agcgtgagca acggcacttt cgccgttttc acgtgtccgt tgccgtgccg
      781 tggcttctcg caaccgtcgc cgttttggcg gcgtgcattc tctgggtgaa gcgcgatcat
      841 aagaccttat tgaccgccga tccaacgaca accgatgttc cacgcggcct gtggggccag
      901 attttcccga ccaagggaca ggaattagcc gtggtcacgg cggatgccgg attcgcgttg
      961 tggcaggatg tcactcacca cactttgaat ctcggcgact atctgagccg gcgatatctg
     1021 gaggaaggtg agcgcccgga aatgcgtgag gtagcggccc gccggacgac cagtcctgcg
     1081 gacctcagcg tgagcttgcg tctggccgag atcgcgagag tcttcggagg ccacgtcaaa
     1141 gtccacttcg cacgcagcat ggatattcac gatttcagaa acggcaatct ggttctggtc
     1201 ggcagccggc gctcgaaccc ctgggtcgaa cttttcgagc gccaaatgaa tttcatcctc
     1261 gattacaatc ccaatttgag aggtccatgt tttctcaacc ggtctcccaa accgggcgag
     1321 tcgaagacct tttctcttcg cagtccctat accgtgcaag ggtccgaaag cgaagcaatc
     1381 gaatcctatg cggtaatcgc gctgttacca aattcctccg ggcatggaaa tgtgatcatt
     1441 ctcgaaggcc tcagcatgga aggaactgaa gccgccgggg aattcgtgac caattcagag
     1501 agattcggca tgttactccg caagatcggc cttaagaacg acgcccccat caagccgttt
     1561 gaggccctgc tcaagttaac agccgtggcg ggcggctatg cggatcccga gctgattgct
     1621 tatcgctatc ctcaatgaat caatttccag ccttggttac gatcactggt ccggtcatca
     1681 gacgagattc aatgggccta taattgatcc gatatgccag ataacttcct gaccccggtc
     1741 acgaggacta ctctgaccag cgatatcgcg cgaaagatgg tcactcacct gattcgcggg
     1801 gactggcagc cgggcgagcg gattccgccg gagcgcgagt tgtgcctgaa actgggcgtc
     1861 ggccgggctt ctctgcggga ggcgctgaag gcgctggaga tcatgggcat gatcgaaatc
     1921 cggctggggg acggcaccta tgtgtgcaac cggtctgagt tcttgtcccg gccgttattg
     1981 tgggcaatcg ccagcagctc acatgccgag gcgcatgagc ttgtggaggc gcggaagctg
     2041 atcgagatcg aactggccgg tctcgccgcc gaacgcgcca ccccacgcga caccaagcag
     2101 atcaaaatgc acctggatca gatggcatcc ggcttgacgg agagcgccgc ttttattcag
     2161 gcggacatcg actttcattt atccatcggg caagccgcgc ataacggcat tttgataaac
     2221 gctctccacc ttatccggaa tctgcttcag gaatggatca acagcacatt gaagatcgag
     2281 ggcgtcgctg agaaggctct ggagcagcat cgttcgattt ttatggcggt ctccaaacga
     2341 aatcgtctgg cggctcgttc ggcgatggag agccacctcg acgagatggc ggctttcctg
     2401 ctcgaggcgc aagaaaggga cacccgggga agccgaacgc cagagtaggc gatcgatacg
     2461 gcgcgcaagc cggaggcggc caggatatac ttgccacttc caatgggttt tctggacagc
     2521 aaaatcatcc tggtaactgg aggcggaagc gggattggac gggagtgcgc gctcaagtat
     2581 tcacgcgaag gcgcttcggt atgcgtggcc gatatcgata gcggcgcagc gaatcgtacg
     2641 tgcgcggagt tagttggcga gaacctggcg gtcgaatgcg atgtgtcctc tggaaccgcg
     2701 tttcgcgagg cggtcgaaga aacggtgcga cggcttggcg ggctcgatgc cgtacacaac
     2761 aacgccggca tcagcagccc ctcgaaaccg ttgcacgaga cctctgaggc ggaatgggac
     2821 gcgcttcttc gcgtgaacgt caagagcgtc tattggagcg cccgcttcgc cctgccgcat
     2881 ttaattgagc gccgcggctc gattctcaat accgccagca tggtagggct gatcggacag
     2941 gcggaccatg ccgcttacgt cgcaacaaaa ggagcgatga tcgctctgac aaaagcgatg
     3001 gctctcgatt atgcgccgca tggagttcgc gtcaatgcga tctgtccggc tggcgtctgg
     3061 acgccattgc tgcggcgctg ggcggccgaa cagcagaatc cagcggagat cgaacaatac
     3121 ctcgatcgca ttcacgcgct cggctattgc cccgagccgg atgtggtcgc cgatgccgcg
     3181 gcattcctga tttcggatca ggctcgtttt ctcactggct gcattctgcc tgtcagtgga
     3241 ggggcggagc tcggctaccg ccggtagagc ttatgagaac aa
//
LOCUS       DATNDT010000403         6067 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1527130, whole genome shotgun sequence.
ACCESSION   DATNDT010000403 DATNDT010000000
VERSION     DATNDT010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1527130"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1299)
                     /locus_tag="VN788_13130"
     CDS             complement(<1..1299)
                     /locus_tag="VN788_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXU21519.1"
                     /translation="MMPIRDVVIFPHMMTPFVVGRESSVRALEEALAGDRKIFLATQH
                     DASVDEPKPNEIYQVGTIVNIVQSLKLPDGNIKVLVEGVERGKVLQIVDTEGYFHASI
                     RVAKYPVESSPVIEQAMQRVTSLFEQYVKLCQSLNYETMIAAVRMEDPSKLTDVIAAN
                     MQLSIEEKQELLEIFDPAERLNRVADVLEIEIEKLNMDRTIQTRVKRQMERAQKEYYL
                     NEKIKAIQKELGRGEKSEWDELKKKVDSAGMPKEVHEKALQELKKLEAMPPMSAESTV
                     SRNYLDWLLAVPWRKKSKEIRNIESAEKILNEDHYGLEKIKDRILEFLAVRQLVKNPK
                     GSILCFVGPPGVGKTSLGMSIAKATGRKFVRLSLGGVRDEAEIRGHRRTYIGALPGQI
                     IQSMKKAGTKNPVIMLDEVDKMAADFRGDPSSALLEVLDPE"
     gene            complement(1491..2762)
                     /gene="clpX"
                     /locus_tag="VN788_13135"
     CDS             complement(1491..2762)
                     /gene="clpX"
                     /locus_tag="VN788_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HXU21520.1"
                     /translation="MKTRSGSEDSLRCSFCHKSQDAVAKLISSPSDYPRAYICDECVA
                     VCNSILEDDRTETHSAAAPNHLPKPQEVKQFLDEYVIGQEQTKKKLAVAVYNHYKRIH
                     MNRNRNGEVELAKSNIMLIGPTGSGKTLLAQTLAKMLDVPFAIVDATTLTEAGYVGED
                     VENIILKLLQAADGDVGRAQTGIIYIDEIDKIGRKDENPSITRDVSGEGVQQALLKIL
                     EGTVANVPPQGGRKHPHQEFTPVDTTNILFICGGAFVGLEKVIGRRIGKKSMGFKAPK
                     EGEAETALAPRRDTEMLAKVEPQDLIKFGLIPEFVGRLPVVGVLDELDENALVEILTK
                     PRNAIIKQYQRLFEFENVRLKFSDEATRAVAREALSRKVGARGLRMILEDLMLDLMYH
                     LPGQKRLKEFEVSKDMVEKRDVSIPMMEKAG"
     gene            complement(3265..3852)
                     /gene="clpP"
                     /locus_tag="VN788_13140"
     CDS             complement(3265..3852)
                     /gene="clpP"
                     /locus_tag="VN788_13140"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HXU21521.1"
                     /translation="MALVPMVVEQTSRGERAYDIYSRLLRDNIVFLGTPVDDQIANLI
                     IAQMLFLAAEDPEKDIQLYINSPGGSITAGMAIYDTMQFVKNDIMTICIGQAASMAAL
                     LLCAGTAKKRFALPNSRILIHQPSMGGLSGQATDIDIHAREILRMREITNHIMSKHSG
                     QKLEKVEKDVERDFIMNAQQSKDYGIVDDIIYKHK"
     gene            complement(3982..5367)
                     /gene="tig"
                     /locus_tag="VN788_13145"
     CDS             complement(3982..5367)
                     /gene="tig"
                     /locus_tag="VN788_13145"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HXU21522.1"
                     /translation="MSQSDTTAETPTEAHIHEPKKLGECEREISVEIAPEAVSGELES
                     FVARYQKMARVPGFRRGKTPASVIRRRFAEELKQEVVESLVPKYFREEVQKLGLQPIS
                     QPRVTDFHFEESEPLRFKAVFEILPAIELAPYNDLRSEKQPVGVTDEEVDRELNSLRE
                     HQASYISVEEDRGLQDGDFAETSFTGTPVKKSESGLVDPAGNPVSSGQGQTPPAGTPP
                     VQMNDVLVDIGGSNTVPEFSDNLRGAKAGEERSFQIAYPEDFQDKRLAGKTLDYKVQV
                     KAIKKKQIPELNDEFARELGDFKSIDELRTRIREQIEAQKKHDVEHAAKDKLVEELVK
                     RNEFPVPESLLERQVTARLERGFRALAAQGMRADDMRKMDFGRLRTGQREAAFREVKA
                     SLILEKIADKENIQTSDEELNTEVERLAHQGRQPVEAVRKKLEEEGALERMRERIRNE
                     KTLDFLYQRSA"
     gene            5835..>6067
                     /locus_tag="VN788_13150"
     CDS             5835..>6067
                     /locus_tag="VN788_13150"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HXU21523.1"
                     /translation="MTSVIWEFKVSAENRQSFESAYDGNGVWAQLFRRDSAYRETILL
                     RDDAEAGRYFTIDVWEDKDSYTKFQEHFADEYK"
BASE COUNT         1194 a         1721 c         1635 g         1517 t
ORIGIN      
        1 ttccggatcg aggacctcga gcagggccga agagggatct ccgcggaaat ccgccgccat
       61 cttgtcgact tcgtcgagca tgatcaccgg attcttggtt ccggcttttt tcattgactg
      121 gatgatctgt ccgggaagtg ctccgatata agtgcgccga tgcccacgaa tttcggcttc
      181 gtcgcgcacc ccacccagcg agagccgcac gaacttacgg ccagtggcct tggcgatcga
      241 cattcccagc gaggtcttgc ccacgcccgg aggtccgacg aagcagagga tcgacccttt
      301 gggatttttt accagttgcc gaacggcgag gaattcgagg atgcggtctt tgattttctc
      361 aagaccgtaa tggtcttcgt tgaggatctt ctctgccgat tcgatgttgc gaatttcctt
      421 cgatttcttt cgccacggaa cggccaacag ccagtcgagg tagttgcgcg aaaccgtaga
      481 ttccgctgac atcggcggca tagcctcaag cttcttcagt tcctgcagcg ctttctcatg
      541 cacttccttg ggcatgcctg cagagtcgac cttcttcttt agctcgtccc actcgctttt
      601 ttcgccgcgc cccagttcct tctggatggc cttaatcttc tcgttgaggt agtactcctt
      661 ctgcgcgcgc tccatctgcc gctttacgcg ggtctggatg gtgcggtcca tgttcagctt
      721 ctcaatttca atctcgagaa catcggcgac gcggttcagt cgctcggcgg gatcgaagat
      781 ttcaagcagt tcctgcttct cttcgattga gagctgcatg ttcgcggcga tgacgtcggt
      841 cagcttggag gggtcctcca tgcggacggc cgcgatcatg gtctcgtagt tcagcgactg
      901 gcagagcttc acgtactgct cgaacaggga agtcacccgc tgcatcgcct gttcaatgac
      961 tggcgaggac tccaccgggt acttggctac gcgaatgctt gcgtggaaat agccctcggt
     1021 atccacaatc tgcagaacct tgccgcgttc tacgccttcc accaggacct tgatgttccc
     1081 gtctggcagc ttaaggctct ggacgatgtt aacgatggtc ccgacctgat agatctcgtt
     1141 cggcttgggt tcatccacgc tggcatcatg ctgcgtggcg aggaagatct tgcggtcccc
     1201 ggccagcgcc tcttccaggg cgcgaacact cgattcgcgt cccactacga aaggcgtcat
     1261 catgtgcgga aagatgacga cgtcccggat gggcatcatg ggcagcttgc gtgtctcaaa
     1321 tttttcccga gtttgggtca cttattcctc ttggtttagc aactctattg taaacagaag
     1381 ccgcgaagac ttggggttaa cacggaggtc acaagggaaa atatcaaggt cacggaagat
     1441 tccgtctcct atctttccga cttcgtgccc ttcgtgttaa cccccgggct ttaccccgcc
     1501 ttttccatca ttggaatcga aacgtcgcgc ttctcaacca tatccttgct gacctcgaac
     1561 tcctttagcc gcttctggcc ggggagatgg tacatgaggt cgagcatcag gtcttcgagg
     1621 atcatgcgca atccccttgc tcccacttta cgcgaaagcg cctcacgagc gacagcgcgg
     1681 gtggcttcat cactgaattt caggcgcaca ttctcgaact cgaagagtcg ctgatactgc
     1741 ttgataatgg cgttgcgcgg cttggtaaga atctcgacca gcgcattctc gtccaactcg
     1801 tcaagaacgc caaccacggg caatctcccg acaaattccg gaatcaggcc gaacttgatg
     1861 agatcctggg gctctacctt cgcgagcatc tcggtgtcac gccgtggcgc cagcgccgtc
     1921 tctgcttccc cttcttttgg ggccttgaag cccatggact ttttgccaat gcggcgtcca
     1981 atgacttttt ccaggccgac gaaagcgccg ccgcaaatga acagaatgtt ggtggtatct
     2041 accggcgtga attcctggtg gggatgcttg cggccgccct gagggggcac gttggctact
     2101 gttccttcca gaatcttgag cagcgcctgc tgcacacctt cgccggaaac atcacgggtg
     2161 atggaagggt tctcgtcctt gcgcccgatc ttgtcgatct cgtcgatata gatgatgccg
     2221 gtctgtgcgc ggcctacatc gccatcggca gcctgcagca gcttgaggat gatgttctcc
     2281 acatcctcgc ccacatatcc cgcctcggtc agggtggtag cgtcaacgat cgcgaacggc
     2341 acatccagca tcttggccag ggtttgtgcc agcagggtct tgccgctgcc ggtaggccca
     2401 atcagcatga tgttcgattt ggccagttcc acctcaccgt tgcgattgcg attcatgtga
     2461 atgcgcttgt agtggttgta aaccgcgacg gcgagcttct tcttggtctg ttcctggccg
     2521 atcacatact cgtccagaaa ctgctttact tcctgcggct tgggcagatg gttgggtgcg
     2581 gcggcggagt gcgtttctgt gcgatcgtct tccagaatcg agttgcaaac ggccacgcat
     2641 tcatcgcaga tgtacgcgcg gggatagtcg ctgggcgacg aaatcagctt ggcaacggcg
     2701 tcctgagact tgtgacagaa ggaacagcgc aggctatctt cggaaccgga acgggttttc
     2761 acgatgactc ctctttaccc tagcgacgat cgacaatcaa tactgcaaag aaccgagata
     2821 ccaacggatt agggtgtccg aactacccgc tcctcgcctc tactacgacc tgtcctcccg
     2881 gccattttcg ccaagagtaa caaaaaacca tcatctttta ttgtttcgga ctcctgtcat
     2941 tttaacgtta ttcgcagcct ccaaggggtc acgagagagg ttccaatgta acttttgcct
     3001 gtgacttttg tgcattaccc gttcggaaag tggaaatcca gaagaagctg ctggcggcta
     3061 gctgctggct gctagctaga accaacacca acacctcaac cacggattgc acggatgaac
     3121 acggattcca cggattcgaa atttcttgtt gttcttcctt gccattccct tctgcatccg
     3181 tgcgatccgt ggcatccgtg taatccgtgg tagagctttt gccgttgcgg ttgacgttag
     3241 ctagcagcca gcagctagaa gcttttattt gtgcttgtaa atgatgtcgt cgacgatgcc
     3301 gtagtccttg ctctgctggg cgttcatgat gaagtcgcgt tcgacgtcct tctccacctt
     3361 ttcgagcttc tgtccgctgt gcttggacat gatgtgattg gtgatctcgc gcatgcgcag
     3421 gatttcgcgg gcatggatat caatgtccgt cgcttgccct gagagcccgc ccatcgaggg
     3481 ctgatgaatc aggatgcgcg agttaggaag ggcaaagcgc tttttcgccg ttccagcgca
     3541 gagcaggagc gcggccatgc tggccgcttg tccgatgcag atagtcataa tgtcgttctt
     3601 cacgaattgc atggtgtcgt aaattgccat gcccgccgtg atcgacccgc cgggagagtt
     3661 gatgtaaagc tggatgtctt tttccggatc ttcggctgcc aggaacagca tctgcgcgat
     3721 gatcaggtta gcgatctgat cgtccaccgg ggttccgaga aagacgatgt tgtcccgcag
     3781 caggcgcgaa tagatgtcgt aagcgcgctc tccacggctg gtttgctcta ctaccattgg
     3841 caccagtgcc attgcagtat tcctttctta gatccttcaa aggagtccgg ccgccctcgg
     3901 ccggattttt tctttagagc tgtgaagata aatcaaagtc cggccgaggg cggccgggct
     3961 cctttgaatt gaaagtagca ctcatgccga gcgttgatag aggaaatcca aagtcttctc
     4021 attgcgaatc cgttcgcgca tgcgctccag cgctccttct tcttcgagtt tcttgcgcac
     4081 agcctcgacg ggctggcgtc cctggtgtgc gaggcgctcc acttcagtgt tcagctcctc
     4141 gtcgctggtt tggatgtttt ccttatccgc gatcttttcc aaaatgagag aggcctttac
     4201 ttctcggaac gcagcctcgc gttgtccggt gcgaagccgt ccaaaatcca tcttgcgcat
     4261 gtcgtcggcg cgcattcctt gcgccgcgag ggcacggaag ccgcgctcca ggcgggccgt
     4321 tacctgccgc tccagcagcg actcgggaac cggaaactca ttgcgtttga ccagctcctc
     4381 gaccagcttg tccttcgcag catgctcaac gtcgtgtttt ttctgggctt cgatctgctc
     4441 gcgaatccgg gtgcggagct catcgatgct cttgaaatcg cccagttcgc gggcaaactc
     4501 gtcgttcaat tctggaattt gctttttctt gatcgctttg acctgaactt tgtagtccag
     4561 agtcttgccc gccaggcgct tgtcctgaaa atcttcggga taggcgatct ggaaagagcg
     4621 ctcttctcct gctttggccc cacgcaggtt atcgctgaac tcggggacgg tgttggagcc
     4681 accaatgtcc accagaacat cgttcatctg caccggcgga gttcccgcgg gcggcgtttg
     4741 tccctgacct gaactgacag gatttcccgc gggatccacc agtccggatt cggatttttt
     4801 caccggcgtg ccggtaaaac tggtttccgc aaagtcgcca tcttgaagtc cgcgatcttc
     4861 ctcgacagag atgtagctcg cctggtgctc gcgtaaactg ttcagttcgc gatcgacttc
     4921 ctcgtctgtg actccgacag gttgtttctc cgatctcaga tcgttgtagg gagccagctc
     4981 gatggcggga agaatttcaa acaccgcctt gaagcgcagc ggctcgctct cttcaaaatg
     5041 aaaatccgtg acccgaggct gggagatggg ctgcagtccc agcttttgta cttcctcgcg
     5101 aaaatacttg ggtaccagcg actcgacaac ttcctgcttc agctcctcgg caaagcggcg
     5161 gcggatcacg ctggctggag tcttgccgcg gcggaatccc ggcacccgcg ccatcttttg
     5221 atagcgagcg acgaacgatt ccagctctcc cgaaacggcc tccggcgcga tctcgacgga
     5281 gatctctcgt tcgcactcgc ccaatttctt aggctcatga atgtgggctt cggtaggggt
     5341 ttcggcagtt gtgtcggatt gactcaagtt cctgcttccg ggttagaaat agccgctcgc
     5401 ctgatcacat cggcgcgcgc ggcctggaaa tctcagtgta agaccgccta aacaaagcgg
     5461 tcaaaccggg aagaagtgca tgccgactcc cccaaaccag ccacacattg gcgtcaagga
     5521 aattcaacac gttcataaat ctgccggctg gtcagattga ctttcggcgc aggggaaaca
     5581 ataaggggaa aagggacgcg ctttgacctt gggcgcttgg gttggatgat caccctattg
     5641 atgcccaaaa gtataagctc ccggactgag cacccttgcg ctgccgcttg ccctttaagt
     5701 ctccgataaa gcctcgccgg aatatcgacc gttgtgcgaa cggcttcgtc tttcattctg
     5761 caaatcacaa tgacacgcgt gtcataaata tggaactagc tacaatttaa ctttcagcgc
     5821 ctaagaggaa ttgtatgacg tctgtcatct gggaattcaa agtctccgcc gagaatcgtc
     5881 agagttttga atccgcctac gatgggaacg gcgtctgggc acagctattt cgccgcgatt
     5941 cggcttatcg cgagacgatt ttgctgcggg acgatgccga agctggacga tatttcacca
     6001 tcgatgtgtg ggaagataaa gattcctaca cgaagttcca ggagcacttt gcggacgagt
     6061 acaagaa
//
LOCUS       DATNDT010000404         3138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1475952, whole genome shotgun sequence.
ACCESSION   DATNDT010000404 DATNDT010000000
VERSION     DATNDT010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1475952"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..1879
                     /locus_tag="VN788_13155"
     CDS             167..1879
                     /locus_tag="VN788_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HXU21524.1"
                     /translation="MAHFRKQDRSLNRRTLLKASVLVGGAAIAQRLFAGPFQNSSRTV
                     GVLPGESRPVVETTAGKVRGSADNGINIFKGISYGASTAGKNRFMPPAKPEPWSGVRD
                     ALRYGASAPQTVPGAPSILGALDFLISAENPRDVGESEDCLVLNVWTPALNDGHKRPV
                     MFWIHGGGFTSGSGSAPLYDGANLARRGDVVVVTINHRLGALGYTYLRDAEGDAFRAS
                     GNAGMLDIVAALEWVRDNIIRFGGDPSRVVIFGESGGGQKVSALLAMPASKGLFQRAV
                     IESGPGLRMVDRAQAAKVGKMFLDELGTNPERIADIQAASLERIMAAQGAVNRKLGPM
                     TPGLIQGFAPVVDGISLLHHPFDPVAPDVSADVPLIIGHNHTEMTLFAGADPSIFSLD
                     DAGLRQRLKPLLKDSADSVLRAFRSTYPRESPSGLYFLITTAYPTVAFTEKIAERRAA
                     LGKAATYVYEFTWPTPIRGGRMRSPHTIEMPFVFDNVRDPLVQKLTGDGADVFPLAEH
                     VCDTWIAFARTGSPNSSNLPHWPPYSAADREVMIINTESRAQRDPDRAAREAIEKFTF
                     GSNA"
     gene            complement(1901..2911)
                     /locus_tag="VN788_13160"
     CDS             complement(1901..2911)
                     /locus_tag="VN788_13160"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU21525.1"
                     /translation="MRLTIMAWTIRAALAVALCLPTMLSLPAMVMAAPPRQKMDEHRA
                     KPIKAMSEAVDVYAIVEGRGGRLIQGLSKDNFELTDDATQQKITNFSQDTNAALSLGI
                     AIDTSVSQGRLLSTEQKAAKIFLSSVLQQADRAFVMNFDVDVKLLSDFTNAPAELARA
                     IDSAEINQTGRSVLPESNARPSGGTHLYDAVYLASNELMKERAGRKVLVLVTDGEDQG
                     SKINLPRSIESAEKADVIVYSIVVSDPDFYSLMGGRYHGAASVRKLAAETGGRTIRLK
                     SIEQIGSAFGQIARELRAQYLLGYFPSNLRHDGSFRRIHVTVRGKSFQVRTRTGYYDH
                     SK"
BASE COUNT          625 a          957 c          896 g          660 t
ORIGIN      
        1 atggtacctt aggacaggta gtgggccagt ttggaaacag accgaaagcc gggcgccaga
       61 tcatgtatcc agatcatgta tagtgacgcg caaatcgagc ggcagtttga aggctcccgg
      121 cctgtcatct agttcccaag gcctgctcct tcaaaggagg ttccagatgg ctcatttccg
      181 caagcaggat cgctctctga atcgccgcac tttactcaag gcttcggtgc tggtcggcgg
      241 cgccgcgatt gcgcagcgcc tcttcgcggg accatttcag aattcgagtc gcaccgtcgg
      301 cgttttgccg ggtgaatcgc ggccagtcgt tgagacgact gcgggcaaag ttcgcggcag
      361 cgctgacaac ggtatcaata tttttaaggg catttcctat ggagcatcga ccgcggggaa
      421 gaatcgcttc atgcctccgg ctaagccgga accgtggagc ggggtgcgcg acgcgctccg
      481 ctacggggcg tccgcgcccc agaccgtgcc cggcgcgccc agcattttgg gggcgctgga
      541 ctttctgatc tccgcagaga atccacgcga tgtgggcgag agcgaggatt gcctggtcct
      601 gaacgtctgg accccagctc tcaacgacgg ccacaagcgg cccgtgatgt tttggattca
      661 cggcggcggc ttcacttccg gttccggctc cgctccgctg tatgacggcg ccaatctggc
      721 gcgccgcggc gatgtggtgg tggtcaccat caatcaccgg ctgggcgcat tgggctacac
      781 gtatctgcgc gacgccgaag gcgacgcctt ccgcgcttcg ggcaacgccg gcatgctgga
      841 cattgtcgcc gccctcgaat gggtccgaga taacatcatc cgttttggcg gcgatccaag
      901 ccgggtggtg attttcggcg aatccggagg cggccaaaaa gtttccgctc tattggcgat
      961 gccagcttcc aaaggactct tccaacgcgc ggtgattgaa agcggccccg gactcagaat
     1021 ggtggaccgc gctcaggcgg caaaggtcgg gaaaatgttt ctcgatgagt tggggacgaa
     1081 cccggaacga atcgccgaca ttcaagcggc atcgctcgag cgcatcatgg ccgcgcaggg
     1141 cgcggtgaat cgcaaactgg ggcccatgac gcccggcttg attcaaggat ttgcgcccgt
     1201 ggtcgatggc atatcgctgc ttcatcatcc atttgatccg gtggccccgg acgtgtccgc
     1261 cgatgttccg ctgatcattg gccacaacca cacagagatg acgcttttcg caggcgctga
     1321 tccgagcatc ttttcgctcg acgacgccgg cctgcggcag cgcctcaaac cgcttttgaa
     1381 agacagcgcg gattcagtgc ttcgcgcgtt tcgctccacc tacccgcggg aaagtccctc
     1441 cggcctgtat ttcctgatta ccacggcgta tcccacagtg gcgttcacgg aaaagattgc
     1501 agagcgcagg gcagctctgg gcaaagcagc aacctacgtc tacgaattca cgtggccgac
     1561 gccgattcga ggcggacgca tgcgatcgcc gcacacgatt gaaatgccct tcgtcttcga
     1621 taacgtgcgc gatccgctgg tacagaaact cacgggcgac ggcgcggacg tttttccact
     1681 cgcggagcat gtatgcgaca catggatcgc gttcgcgcgc accggcagtc caaattcgag
     1741 caatctcccg cactggccgc cctattcagc tgcggatcgt gaagtaatga tcatcaatac
     1801 cgaaagccgg gcgcagaggg atccggaccg ggcagcccgt gaagccatcg agaagttcac
     1861 ttttggctcg aacgcttaat gacccaggaa gcggaaggtg ctatttgctg tggtcatagt
     1921 atcccgtccg cgtccggacc tggaaacttt tcccccggac cgtcacgtgg atacgccgaa
     1981 acgagccgtc atggcgaaga ttcgagggga aatagcccag cagatattgc gcgcggagct
     2041 cacgcgcgat ctggccaaat gccgatccga tctgctcgat ggatttcagc cgaatcgtgc
     2101 gcccacccgt ttccgcagca agcttgcgca cactcgcggc gccgtgatac ctgccgccca
     2161 tcaatgaata aaaatcggga tcgcttacga caatcgaata tacgatcaca tcggctttct
     2221 cagccgattc gattgacctt ggcaagttga tcttgcttcc ttgatcctcc ccgtcggtca
     2281 ccagcaccag caccttgcgt ccagcgcgtt ccttcatcaa ttcattagaa gccaggtaca
     2341 ctgcgtcgta aaggtgcgtg cctccgctgg gacgggcgtt gctttccggc aacacgcttc
     2401 gtcctgtttg attgatttcg gccgaatcaa tcgcccgcgc caattcggcc ggcgcgttcg
     2461 taaaatcgct cagcaacttc acgtcaacat cgaaattcat cacaaacgcg cgatcggcct
     2521 gttgaaggac ggagctaaga aatatcttcg ccgccttctg ctccgtgctc agcaggcggc
     2581 cctggctgac gctggtatcg atggcgatgc ccaaactcag cgccgcattc gtatcctgcg
     2641 aaaaattagt gatcttttgt tgcgtcgcat cgtcggtaag ttcgaagttg tccttgctca
     2701 aaccttgaat caatcggccg ccacgccctt cgacgatcgc gtacacgtca acggcttccg
     2761 acatcgcctt aattggcttg gccctgtgtt catccatctt ctgccgcggc ggtgcagcca
     2821 tcaccatagc cggcaaacta agcatggtcg gcaaacaaag tgcgaccgca agggctgctc
     2881 ggatcgtcca tgccattatg gttaaacgca tgcgtcaggc cgtcggctgg tgagcaagtt
     2941 cacggcgctc cttcgctcac atcctacgcg cggctttcct cccagtcgaa tggtgcaaaa
     3001 aaaaaggccg gactatcggg ctgccgctct ttgcgtgcgg tcctacccgt gtccggccct
     3061 gggtattcga caaccttgag tcaaataacg gcgggtggcg gccttactcg ccgcttacct
     3121 gcaccggcac cgtttcga
//
LOCUS       DATNDT010000405         3918 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3069238, whole genome shotgun sequence.
ACCESSION   DATNDT010000405 DATNDT010000000
VERSION     DATNDT010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3069238"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /locus_tag="VN788_13165"
     CDS             complement(<1..1356)
                     /locus_tag="VN788_13165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU21526.1"
                     /translation="MKTTADSKANTKEVGWSSMNGRRIFGIFGKVILPVGCVLLCLTL
                     LGAQQSSTKPTDNAAAANHDHATADHVKTEGPDHHASAGANHAKAAPARMSDAASPEL
                     PHVTWSDDGGGPDNSHYVDFKQITKANVGKLQVAWAYPSNDTISYVWNPLIVGNVMYV
                     LARNDSLVALDATTGKEIWVHEQLQGIEPRGINYWESKDHTDKRLIFARAGFLEEINA
                     ATGKSILTFGTDGIVDLHVGLGRYSTGGPLGRIQSNNPGKIFENLLIEGSATGEGFMS
                     PPGDFRAYNVITGALVWQFHTIPHPGEYGYDQWPKDAWRYAGGANTWGEITIDAKRGI
                     AYFPTGSATYDFYGADRAGNDLYGDCLIALDARTGKRLWYYQTVHHDLWDYDNTAAPQ
                     LITIEHNGKKIDAVAMAGKTGFLYVFDRVTGKPIWPIVERPVAKSDMPGEHASPTQPF
                     PT"
     gene            complement(1472..2392)
                     /locus_tag="VN788_13170"
     CDS             complement(1472..2392)
                     /locus_tag="VN788_13170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21527.1"
                     /translation="MKRREFIAGGLAGGLAFAVGSRVKAQAGGASEAEKLARLSVMTY
                     CFDRIVKGLGKPDDPDRTLDILDAPQMIADHYGIHHVEMQHTHFQATDKPYFQEMKGR
                     LKKANSQLTQICLEFDFVDLSSKQKYQQLEMIALSKDWIDYCVQLGCPRVLLNQGPLT
                     PDVVEDAKWTLKQITDYAKTQKVFCDIENRGGGRPAPNVPPNAPVRGGWEVLVEVLKA
                     GGGYANPDIGNFPDEESRAAGLRVMYAMTSGNSHAHYAPDRYDETKAIGIAKDVGYKG
                     LFSIETSARNNGPDPYAAVQSVRDALLKII"
     gene            3092..3664
                     /locus_tag="VN788_13175"
     CDS             3092..3664
                     /locus_tag="VN788_13175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HXU21528.1"
                     /translation="MSETSNMLPLGTIAPAFRLRDPHGKLVSSDDFKNAPALLVAFIC
                     NHCPYVKHIAGAFSHLASEYQARGVAVFAINSNDAETYPEDGSEKMAEEIQHYGYTFR
                     YLVDETQDVARAYQAVCTPDIFLFDKDRRLVYCGQFDDSRPKNDRPVTGADLRAALDA
                     VLAGRTVAQDQKPSVGCSIKWKLPKATAAR"
     gene            complement(3661..>3918)
                     /locus_tag="VN788_13180"
     CDS             complement(3661..>3918)
                     /locus_tag="VN788_13180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21529.1"
                     /translation="LGFLPQISHASAMIFVTPNKTYGFVALDAIRLYRTPKGLTLVKC
                     SFNRDCELTPGNAGAAERPHTATGRSAPDESASGVTKAKRC"
BASE COUNT          771 a         1320 c          934 g          893 t
ORIGIN      
        1 cgtcggaaaa ggctgcgtcg gtgacgcgtg ctcgccgggc atgtcgctct tcgctaccgg
       61 ccgctccacg atcggccaga tcggtttgcc cgtcacgcga tcaaacacat aaaggaaccc
      121 cgtcttgccc gccatcgcca cggcatcgat ctttttgccg ttgtgctcga tcgtgatcaa
      181 ctgtggcgcg gcggtattgt cgtagtccca aaggtcgtga tgcaccgtct ggtagtacca
      241 caatcgtttt ccggtccgcg catccagtgc gatcaggcaa tcgccataaa gatcgtttcc
      301 cgcacggtct gctccataaa aatcgtacgt tgccgaaccc gtcgggaaat atgcgatccc
      361 gcgctttgca tcgatcgtga tctctcccca cgtattcgcg ccgcccgcgt atcgccatgc
      421 atccttcggc cattggtcat acccgtactc gcccggatgc gggatcgtgt gaaattgcca
      481 cactagcgcg ccggtaatca cgttgtatgc gcggaaatct cccggcggcg acataaatcc
      541 ctcgcctgtc gccgaaccct cgatcagcag gttctcgaaa attttcccgg gattgtttga
      601 ttgaattctt cccagcggcc cgcccgtcga atagcgaccc agcccgacat gcaaatcgac
      661 aattccgtcc gtgccaaacg tcaatatcga cttccccgtc gccgcattga tctcttccaa
      721 aaagcccgcc cgcgcgaaga tcagccgctt gtccgtatga tccttgctct cccaataatt
      781 gattccgcgc ggctcgatcc cctgcagctg ctcgtgcacc cagatttctt ttcccgtcgt
      841 cgcatccagc gccacaaggg aatcgttccg cgccaacaca tacatcacgt tgcccacgat
      901 cagcggattc catacatacg agatcgtgtc gttgctcgga tacgcccacg ccacctgcag
      961 cttgccgacg ttcgccttcg tgatctgctt gaagtccaca taatgcgaat tatccgggcc
     1021 gccgccgtca tcgctccacg tcacgtgtgg cagctccggc gatgccgcat cgctcatccg
     1081 cgcgggagca gctttcgcat gattcgcccc agcactcgcg tgatgatccg gcccctcagt
     1141 tttgacgtga tcagctgtgg cgtgatcgtg atttgcagcc gcggcgttat cagtcggctt
     1201 tgtcgaactc tgctgcgcgc ccagcaacgt caggcacagc agcacgcaac caacaggcag
     1261 gataaccttg ccaaatatcc cgaagattct tcgcccattc atcgacgacc accccacctc
     1321 tttcgtattc gcctttgaat ctgccgttgt cttcatagcg gccaccgctc tgccctgagc
     1381 acgcgaaggg tgcgcgcgtc ttttgcgcgc ggttctcggt gggcgctttt gaattttctt
     1441 tgtcgtcgcc tctgtatcgg ttgccgcgat cctaaataat cttcagcaac gcatcccgca
     1501 cgctctgcac cgccgcatac ggatcgggac cgttgtttct ggcgctcgtc tcgatcgaaa
     1561 atagcccctt gtaccccacg tctttcgcga tgccgattgc tttcgtctcg tcatagcgat
     1621 ccggcgcata atgcgcgtgg ctgttgcccg aagtcatcgc atacatcacg cgcaaccccg
     1681 ccgcgcgcga ctcttcatcc gggaaatttc cgatatccgg attcgcatag cctccgcccg
     1741 ccttaagcac ttccaccagc acttcccaac cgcctcgcac cggcgcattc ggcggcacat
     1801 tgggcgcagg acgcccgcct ccgcgattct ctatgtcgca aaagaccttt tgcgtcttcg
     1861 cgtaatccgt gatctgcttc agcgtccact tcgcgtcctc caccacgtcc ggcgtcagcg
     1921 gtccctgatt cagcagcacg cgcggacatc ccagctgcac gcaataatcg atccagtcct
     1981 tgctcagcgc gatcatctcc aattgctgat atttttgctt tgacgataaa tctacgaaat
     2041 cgaattccag gcagatctgc gtcagctgcg aattagcctt cttcaaccgg cctttcatct
     2101 cctgaaaata tggcttgtcc gttgcctgaa aatgcgtgtg ctgcatctcc acgtgatgaa
     2161 ttccgtagtg atccgcaatc atctgcggcg catccagaat gtccagcgtc cggtccggat
     2221 cgtccggctt gcccagtccc ttcacaatgc ggtcgaagca ataggtcatc accgaaagcc
     2281 gcgccagctt ctccgcttcc gaagcgccgc ccgcttgcgc cttcactctg ctccccaccg
     2341 caaacgccag tccccccgcc aacccgcccg caataaactc acgccgtttc atagctcacc
     2401 cccagaacgt tttctctgct gtatacccca tccggcagaa ccttttctcc cgtcatgcaa
     2461 gcccgatcca aagcccaccc atagaagccc gacgcaagcc cgcgccccgt aaacttaacc
     2521 cgaaaacctc gcccacagca ttttcatcac gtgaaatggc ttttcgcaaa aatcgcacgc
     2581 cggagtaata cacccgtacc gttcctcacg caagcacctt cgcaaacttc tctttctgat
     2641 tatcggcaat cccaaattga acccgctgcc atcacgggcg cgcaggttgt cgagtcgtcg
     2701 gtttagaaat tgtcggacct ctcttgcagt tactgttgcc tcgcgtagca gccacagtcc
     2761 tgccctgagc acgcgaaaag tgaccacgca ttttgcgcgc gtccctcgct ggcgccttgc
     2821 atttgctttg tttcggtttt gtctgcagtg gcggccttcg ttcggtgatt cgcaggtacg
     2881 acgcgtgttc tctacaaagg cagcagctct tggctcggtc gcccccgatt ggagtcgaat
     2941 gcagttgaat cgttcgtgcg tacagaaaat cggtcgatag cgtctggcag tcacgccatt
     3001 tcacagattc acccgctgtt ccccagtaag ctgttttcga atcaccaacc caagcaggca
     3061 ggtgctaatc tattcgcgga ggtacgaatt aatgtccgaa acttctaata tgctcccgct
     3121 gggcactatc gccccggcgt tccgcctgcg tgacccgcac ggcaaactgg tctcgtccga
     3181 cgattttaag aacgcgcccg cacttctcgt cgccttcatc tgcaatcatt gtccttacgt
     3241 aaagcatatc gccggcgcct tttcgcatct cgccagcgaa taccaagcac gcggcgtcgc
     3301 cgtgttcgca atcaattcca atgacgccga aacgtacccc gaagacggct ccgagaaaat
     3361 ggccgaagaa atccaacatt acggctacac gttccgctat ctcgtcgacg aaacgcagga
     3421 cgtcgcccgc gcctaccaag ccgtctgcac gcccgatatt ttcctgttcg acaaagaccg
     3481 ccgcctcgtc tattgcggcc agttcgacga cagccgcccc aagaacgatc gcccggtcac
     3541 tggcgccgat ttgcgcgccg cgctcgacgc cgtcctcgcc ggccgcaccg tggcccaaga
     3601 ccaaaagccg agcgtaggct gcagtatcaa atggaaactc ccaaaagcca ccgccgcccg
     3661 ctagcagcgc tttgcttttg taacgcccga cgcgctttcg tcgggcgcgg atcttccggt
     3721 tgctgtatgt ggccgctccg ccgcccccgc gttacccggc gtcagttcgc aatctcgatt
     3781 gaagctacat ttaaccagcg tcaggccttt cggagtgcgg taaagtctga tagcatctaa
     3841 tgctacaaat ccataggttt tgttgggagt aacgaagatc atggcactcg catgtgaaat
     3901 ctgcggcaaa aagccgag
//
LOCUS       DATNDT010000406         5138 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_411843, whole genome shotgun sequence.
ACCESSION   DATNDT010000406 DATNDT010000000
VERSION     DATNDT010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5138
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411843"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..284
                     /locus_tag="VN788_13185"
     CDS             <1..284
                     /locus_tag="VN788_13185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21530.1"
                     /translation="PTPRGSALVIFLRRDNSRSAGDRAPRRFLPLALVVLRAQQSRLP
                     TTKRSLTPPRSGRKSSLLATVHLVFGGDASGSSATSSGPDYWPQSTMAD"
     gene            480..701
                     /locus_tag="VN788_13190"
     CDS             480..701
                     /locus_tag="VN788_13190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21531.1"
                     /translation="MARQFLGVTPRRWLEYLVAILLGNAIYYFSLVPYLPDSLRHQGF
                     SIDGGVLVDFVVCVLVYGLIRLGVRVRRG"
     gene            complement(738..2549)
                     /locus_tag="VN788_13195"
     CDS             complement(738..2549)
                     /locus_tag="VN788_13195"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU21532.1"
                     /translation="MRKQAEDKTFAISGPALPEAGRRVRISGRIQTGIVACPTFLWGA
                     RMVVGSVPLIGERRSFVLTNPNSTSGGFTGTSERRAHPRSRPQPFAYVKVGEHNGGIV
                     MDASENGVQVAAANSLAPEKVVRLSLQLSASAEPVEVEARVVWLSESKKTGGFEFVSL
                     TEEARNQIREWLNGDASKARSSGPKAVVIPARDQRIAEVRAEARANDANLEVRTPSVP
                     DFPPKGFERMFPPEGEPIGPGSVREDEPRNGRAIDEHPADTGMGRDRPAPFKRSHAGE
                     RPLEPGPFQEREPLRPLRTESRFAARAIFSDSPFQAPPSEPRRLTNARMGLLTGVVMV
                     VCFAVGMIAGRVWMGRWPQGWNLQRLTGGTSAPAPTPPPPAAATAPAENPTANLDANP
                     TANPDTIPKATPDADGSPAAAPASTPPAVATPAPPESSGAKSDAGATPIPTVKQNPAA
                     ASAERLPPELAGSSILVTAPAAGGRSVRVSLPQEPLTASASVAIGVRRAALLPPQPGP
                     VSAHAPERLEFGKIIAQVPNVLSPNIPPMGDPIVVLRATIGKQGEIKSLVPIRGRDEL
                     IPTAERLIRGWQQFPTRLSGQPIESTEEIILTFRTGP"
     gene            2594..3592
                     /locus_tag="VN788_13200"
     CDS             2594..3592
                     /locus_tag="VN788_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21533.1"
                     /translation="MKLRSIFCLRVFAISFLVFWMATFAQSSAAQPVATGAASCDRAC
                     LEGMVDKYLNAMVAHDASRAPLSPTVKFAQDNVPLKIGTALWTTATALGTYRHYFADP
                     ERGDVGIIGVIYENGVGAILILRLKVENHLITEAEQFVAHDPRGAALYEKMGKPNPGW
                     LTTIPPDRRQSREALESTAYMYYEALEKNDGRGIYPFTPDCSRIEDAVPTTNQPKPEN
                     YGHSDEDISDFTMLGCKAQYELGFLGFTTGCRDRRFLVVDTERGSVLASAYLDFDGTV
                     KEVHLTDGRVWKVPPYFMTPRTNQSNEAYRIENGSIRLIEMTMYEVPFNAQPAFGN"
     gene            3607..4692
                     /locus_tag="VN788_13205"
     CDS             3607..4692
                     /locus_tag="VN788_13205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21534.1"
                     /translation="MFKTFSRIALLSAWCVAICGVASTPSLAAPDHGKSLVYLPPPAS
                     PASSTPCDRACLNAFVDKYFNALISHCTCNVPFAPDVKYTENGESVKPGEGIWKTFTG
                     RGTYRVYLADPATGEAGYYGDIKEFGTLLGTMALRLKVNDRRIAEVEVVVARQQLRPK
                     GGLGLNTAGIMTPRMIDELTPAGFVSPDSTLLEPLPAANRSPRERLIAITDQYFQAFS
                     QNKSSAAPFANSCSRRENGFAATNNSDGPVVDPAQPAFRVFSQGCAQEIDRGFFSSLS
                     KMGDHRPLVVDVDQGLVLDLAFLDNEGNVKSVSVPGVGDVKVPAEFLRPITFMQPQLF
                     KIEAGKIREIEGLSWPMPYGMRSGWEK"
     gene            4823..5098
                     /locus_tag="VN788_13210"
     CDS             4823..5098
                     /locus_tag="VN788_13210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21535.1"
                     /translation="MPADEPSTMWVPVCCGRVMRCNMFRQADGGAYAALVCTVCNKTI
                     TLEQEPLAAANSFGEGSKVLSLIGSPKPPKTDRRKAFTEAGPDDPTL"
BASE COUNT         1050 a         1539 c         1442 g         1107 t
ORIGIN      
        1 ccccaacgcc gcgcggctcg gcgctcgtga tattcctgcg tcgtgacaac tcgcgcagtg
       61 ctggcgatag agcgcctcgc cgctttctcc cgctggccct cgttgtcctg cgcgcgcagc
      121 aatcgcgcct gccaacaacg aaacgctcac taacaccgcc ccgctcagga cgcaaatcct
      181 cgctgctcgc aaccgttcac ctcgtttttg gcggcgatgc atctggcagt tctgcaactt
      241 cttcgggtcc tgattactgg ccgcaatcca caatggccga ttgaactcgc ggccattgtg
      301 catagtcgac gatgggaagg caactattct gagtcgtcaa tccgaacccg cggttatagc
      361 agcggtttac aggcgtcact cgccgcacta tattgggatt tcgtctgatc ggctggctta
      421 aagctgggcg cgggcgtgag tattctcaag gccggctgtc cgaggcaact gatagataaa
      481 tggcgcgcca atttcttgga gtcactcccc gccgatggct ggaatatctg gtcgcgatcc
      541 tcctcggcaa cgccatttat tacttctcgc tcgttccgta cctgccggac tcgttacgtc
      601 atcagggatt ttctatcgat ggcggagttc tcgtcgattt cgtcgtttgc gtgttggttt
      661 acggtctgat ccggctgggc gtccgcgtgc gccgcggtta ggcgtggaag cgacgccgaa
      721 atcgtgttcg cggttttcta gggtccggtt cgaaaggtca ggattatttc ttccgtggac
      781 tcgatgggct gcccgctcag ccgcgtcggg aactgctgcc acccacgaat cagtctttcc
      841 gctgtcggaa taagctcatc acgtccccgt atcggcacaa ggctcttgat ttccccttgc
      901 ttgccgatgg tggcccgtaa aacgactatg gggtcgccca tgggcgggat gttgggagaa
      961 agaacattcg gtacctgcgc aatgattttg ccgaattcga ggcgctcggg agcatgagcc
     1021 gataccggtc ctggctgtgg cggcaacagg gctgcgcggc ggacgccaat tgccaccgat
     1081 gccgaagcgg tcaaaggttc ttgcggcaag gacacacgca cggaacggcc acccgcagcc
     1141 ggagcggtaa caagaataga gcttcccgcg agttcggggg gcaaacgttc cgcactcgcg
     1201 gcagctgggt tttgtttcac agttggaatt ggagttgccc cggcgtctga ctttgcaccc
     1261 gagctttcag gcggagcggg agtagcaacg gcaggcggag tggaggctgg cgcggcggct
     1321 gggcttccgt ccgcatctgg agttgccttt ggaattgtgt cgggatttgc cgttggattt
     1381 gcatcgagat ttgctgttgg attttcggcc ggtgcggtgg ccgcggcagg cgggggtggc
     1441 gtcggagctg gcgcggatgt gcctccagtc aaccgctgca ggttccaacc ttgaggccat
     1501 ctccccatcc agacccttcc ggcaatcatg cccaccgcaa aacaaacgac catcaccacg
     1561 ccggtcagca aacccatgcg tgcgttcgtc aatcgccggg gctcgctagg aggagcctga
     1621 aaaggtgaat ctgaaaaaat tgcccgggcc gcgaagcgcg attcagtgcg cagaggtcgt
     1681 aaaggctcgc gctcctgaaa tggacccggc tctagcggac gctcaccggc atgacttcgc
     1741 ttgaaaggcg caggacggtc gcggcccatg ccggtgtccg ctggatgttc atcgatggcc
     1801 cttccattac ggggttcatc ttcgcgaaca gaacccggac ctatcggctc accctcgggt
     1861 ggaaacatcc tttcgaatcc tttgggaggg aagtctggaa ctgagggcgt tcgaacctct
     1921 aaatttgcgt catttgctct cgcctcagct ctgacttctg ctattcgctg atctcttgcc
     1981 ggtattacca cggctttggg cccggaagac cgcgctttac tcgcatctcc gttgagccac
     2041 tcgcggattt ggttccgggc ctcctccgta agcgacacga actcaaagcc gcccgttttc
     2101 tttgactccg agagccagac aaccctggct tccacctcca cgggttcagc agaagcgctt
     2161 agttgcaagg agagccgaac gaccttctcc ggggccaaac tattggccgc cgccacctgc
     2221 acgccgttct cactcgcgtc catgacaatg ccgccattgt gttcacccac cttgacatag
     2281 gcaaaaggtt gcggcctcga gcgagggtgc gcgcgccgct cactcgtccc cgtaaaacct
     2341 ccggacgtac tattagggtt cgtcagcaca aaacttcttc tttcacctat cagcgggacg
     2401 cttccaacca ccattctagc tccccaaagg aacgtcggac aagccacgat gccagtctgg
     2461 attcttccgg agatacgaac ccggcgtccc gcttctggaa gtgctggacc cgaaatggca
     2521 aatgtcttat cctccgcttg cttccgcatg caacattccg gaatctgctc tccattgggc
     2581 ttgagaggaa atcatgaaac tgagaagcat cttttgcctg cgggttttcg caatatcatt
     2641 cctcgttttc tggatggcga cgttcgctca atcatcggcg gcgcagcccg tggccacagg
     2701 ggccgctagc tgtgaccgcg cttgcctcga aggaatggtg gacaaatacc tgaacgccat
     2761 ggtcgcgcac gatgcatcgc gcgctccgct ttcgcccacc gtaaaattcg cgcaggacaa
     2821 tgtccctctc aaaataggca cagcgctgtg gacaacggcc accgctctcg gcacctaccg
     2881 ccattatttc gccgacccgg aacgcggaga cgtcgggatc atcggcgtaa tctacgaaaa
     2941 cggcgtgggc gccatcctga tcctgcgcct gaaagtcgaa aatcacctca ttaccgaggc
     3001 ggagcagttc gtggcccacg atccccgtgg tgcagcgctc tacgaaaaga tgggcaagcc
     3061 caatccgggt tggctcacca ctatcccgcc cgatcgacgc caatcgcgtg aggctcttga
     3121 atcaacggcc tacatgtact acgaagcact cgagaagaat gatggcagag gcatttaccc
     3181 cttcacgccc gattgcagcc gcatcgaaga tgccgtcccc accaccaatc agccgaagcc
     3241 ggagaattat gggcattcgg acgaagatat cagcgatttc actatgctcg gttgtaaagc
     3301 tcagtacgag ctcggttttc tcggtttcac cacgggatgc cgcgatcgcc ggtttctcgt
     3361 ggtagacacg gagcgcggat cggtcctcgc ctcggcctat ctcgatttcg acggcaccgt
     3421 gaaagaagtg catcttacgg acggccgcgt ttggaaagta cctccttatt tcatgacgcc
     3481 gcgtaccaac cagtcgaacg aggcatatcg aatcgaaaat ggaagcatcc gcctcataga
     3541 aatgaccatg tacgaagtcc cattcaatgc tcagccggct ttcggcaact aggctcggag
     3601 ataatcatgt tcaaaacttt ttcgcgtatt gcactgctta gcgcgtggtg cgtcgccatt
     3661 tgcggtgtcg catcgacgcc cagccttgcc gcgcccgacc acgggaagtc gctggtgtat
     3721 ctgcctccgc cagccagccc ggccagttcc acaccgtgcg accgcgcctg cttgaacgcc
     3781 tttgtcgata aatatttcaa cgcattgatt tctcactgta cttgcaacgt tccctttgcg
     3841 ccggacgtga agtacaccga aaacggcgag tcggtgaagc ccggcgaggg catttggaaa
     3901 actttcaccg gacgcggcac ataccgcgtc tatctcgccg atccggcaac gggtgaggct
     3961 ggttactatg gagatataaa agaattcggt acgcttctgg gaacaatggc cctgcggctg
     4021 aaagtgaatg atcgtcgcat cgccgaagtg gaggtggtcg tggcccggca gcagctgaga
     4081 ccaaaaggtg gcctgggcct gaacaccgcc ggcatcatga cgccaagaat gatcgacgaa
     4141 ctgacacccg cgggctttgt gtcgccggat tcaactctgc tcgagcccct gcccgccgcc
     4201 aatcgctcgc cgcgggaaag attaattgcg atcaccgatc aatatttcca ggccttttcg
     4261 caaaataaaa gctcggccgc gccgttcgcc aatagttgct cccgcaggga aaacggattc
     4321 gccgccacta acaattccga tgggcctgtc gtggatccag ctcagcccgc tttccgtgtg
     4381 ttcagccaag gttgcgcgca ggaaatcgac cggggattct tcagctctct ttccaagatg
     4441 ggcgaccacc gcccattggt cgtggatgtc gaccaaggcc tcgtattgga cctcgcattt
     4501 ctcgataacg aaggcaacgt gaaatcggtt tcggtgccgg gagtgggcga cgtcaaggtt
     4561 cccgcagagt ttctcaggcc aatcaccttt atgcagccgc aactattcaa aatcgaagcc
     4621 ggcaagatcc gggagatcga aggcctttct tggcctatgc cctacgggat gagatccggc
     4681 tgggaaaagt agtcgcatga tcggcggcga tcttctctaa cacaaacacg cgcgcgaagt
     4741 cgccgttcag ctcgttgcgt gaatcttggc aagcaggttc cattgtataa tcctcaggca
     4801 ttcgcatccg gcgaggagac tcatgcccgc ggacgaacct tccacaatgt gggttcctgt
     4861 atgttgcggg cgcgtcatgc gctgcaacat gttcaggcag gcggatggcg gggcttatgc
     4921 agcgctcgtt tgcacggtgt gcaacaaaac gattacgctc gagcaagagc ctctagcggc
     4981 cgcgaatagc ttcggagaag gctcgaaagt tttgagcctg attggatcgc ccaaaccgcc
     5041 aaagacggac agaagaaaag cgttcaccga agccggcccc gacgatccaa cgctctagcc
     5101 tgaattgcgg ctcgcccaaa actttgccgg ccaatgat
//
LOCUS       DATNDT010000407        10315 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3540206, whole genome shotgun sequence.
ACCESSION   DATNDT010000407 DATNDT010000000
VERSION     DATNDT010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3540206"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="VN788_13215"
     CDS             complement(<1..352)
                     /locus_tag="VN788_13215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21536.1"
                     /translation="MVAANLVTKILAVALATQGTCVDACTTPPPQPAQATMTQRMQSL
                     ANEARLFSLAVLPAFELVPSVVIESKLFGNAKHEGGFSCQEYKIFLPENFDADPRKGD
                     VIIAHELSHAQDCLT"
     gene            1044..1376
                     /locus_tag="VN788_13220"
     CDS             1044..1376
                     /locus_tag="VN788_13220"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HXU21537.1"
                     /translation="MPGLGPRAYCLSVDGDGLRPRFAKGDVVIVDPDRMPQPGDMVVL
                     YGNAKIAAIAEHDGHPKPVPCNRRKGVPFAVIGEHDASAVRWRFSRRGGMQPVGGGAR
                     KGAANDFR"
     gene            1363..1539
                     /locus_tag="VN788_13225"
     CDS             1363..1539
                     /locus_tag="VN788_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21538.1"
                     /translation="MTFAEIVLIVALALAILGGWVGYRRAFASGYLHGYNASALGDPP
                     EPQSAAVNSLRRIF"
     gene            1857..2126
                     /locus_tag="VN788_13230"
     CDS             1857..2126
                     /locus_tag="VN788_13230"
                     /inference="COORDINATES: protein motif:HMM:NF019225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU21539.1"
                     /translation="MTKTVNALYLITAMIMMSGPAFASAGCCNPIPVPEPSSLTILAA
                     GLAAGGIGVFLQKRFRRSGRLVGLLCFATAAATLWASIGVAGACC"
     gene            complement(2696..3142)
                     /locus_tag="VN788_13235"
     CDS             complement(2696..3142)
                     /locus_tag="VN788_13235"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU21540.1"
                     /translation="MQRQRVPAHRSSAGDQLVISDAVAVTLTWGGVALDITEEIGNNG
                     PALNGINVELGLAASNSLNSDESLSSEIASIHIPFSGGPSGFYTVYDDSLAPGAYVLA
                     GSNVGDPNYQTDFAPGTTSVPEPASLAVLGASLVGFSAMGRRRRVA"
     gene            complement(3316..3516)
                     /locus_tag="VN788_13240"
     CDS             complement(3316..3516)
                     /locus_tag="VN788_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXU21541.1"
                     /translation="MCLEHEFWQALYEIAAENGTTRSRLISRLLEQERDFNFTSTVRV
                     FVLRHFRRKLMEQIADNIPEIV"
     gene            complement(4077..4463)
                     /locus_tag="VN788_13245"
     CDS             complement(4077..4463)
                     /locus_tag="VN788_13245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21542.1"
                     /translation="MAIAVTQRHVEDVIDRAIAPVSVRVSAGEPLQFVAIGSAERSQN
                     VVLTFRDYEEIAERICAALAESGYLASSSDIPIVFDKIMQGWSLKLKSKSKACLAGSI
                     PNNIYSLLQNSGVLKEFVSDSVLLPA"
     gene            5046..5717
                     /locus_tag="VN788_13250"
     CDS             5046..5717
                     /locus_tag="VN788_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21543.1"
                     /translation="MYYGSGNSNTAITVDSNNGVQLGESAITRFVGPIAPTPSNSNVY
                     EVPLGDTMVAGKTGANWGIFFGVDLTGTGLSVNDITATWTLTDLARGTIGSFNPLLIP
                     DNALLFSDGSTFNGNGTRPSGKGPPVGAENSEALSFASIAGAFDDPSFSDNINDTFYL
                     TLTVDRVPACAICSPTQLASDEIVINQGTAIDEPSSIAILGLGLLAAGCFGLLRIKRK
                     DGLPA"
     gene            7222..7578
                     /locus_tag="VN788_13255"
     CDS             7222..7578
                     /locus_tag="VN788_13255"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXU21544.1"
                     /translation="MHNDLKSLLANQIEHRNSTLLVSTANGRPYKIQGFQSMFFRLIR
                     SLVARQLVQPGLSFHGLRHTVGTKLAEAGCDARTIAAMLGHSTTHMAEHYSRNADRKE
                     LVREAVQRLEQRSRNA"
     gene            7834..8025
                     /locus_tag="VN788_13260"
     CDS             7834..8025
                     /locus_tag="VN788_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21545.1"
                     /translation="MGKPLAAVYISPVSGMPKIAAIVQDGQVTYAQTVADEVEGKVLL
                     SDMLAILDPPNAPKTKGPR"
     gene            8360..8548
                     /locus_tag="VN788_13265"
     CDS             8360..8548
                     /locus_tag="VN788_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21546.1"
                     /translation="MRDRCRTTTWLNLVSLNGGKRGNVDGLPLLHIGMLFFKRAVIGA
                     VNLNETCYFVFYEMLTWV"
     gene            8645..9547
                     /locus_tag="VN788_13270"
     CDS             8645..9547
                     /locus_tag="VN788_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21547.1"
                     /translation="MTTRALFRFTGPEKSQVACIDKGNAWAVQSQTEFLSANGGNTTV
                     AWLWDALPYVPKPMDDYPELEAALEKVRHYLTEMQPVREKVALHKSQGILADETELLG
                     KIVEAHERLISMYLAKAVKLFEARRPLASQKNAVGSLTRGQKGGATLAVRNDDLPVET
                     PSSLEDGSTPRPERMALKSKELKDGCVALITKILNQTLPEDSGAHLPSTAIEDLTQQM
                     IRTVFEPRGRLRERARRDPRRMTEIVGLATLPIVTRGNRDNVAPTLVDMLEHANLVSF
                     DVELSDADNWPRLKARAQRGLTVA"
BASE COUNT         2229 a         2783 c         2876 g         2427 t
ORIGIN      
        1 cagtcagaca atcctgcgcg tgactaagtt catgcgcaat aatcacgtct cctttccgag
       61 gatccgcatc gaaattttcc ggaaggaaga tcttatattc ctggcaagag aatcccccct
      121 catgcttggc attcccgaaa agttttgatt cgatcacgac agacggcaca agctcgaacg
      181 cgggtagcac tgccaaagaa aaaaggcgtg cttcgttggc caacgattgc atgcgttgcg
      241 tcatcgtcgc ctgcgcgggt tgcggtggcg gcgtcgtgca agcgtcgacg catgttcctt
      301 gcgtcgccaa tgccactgcc aggattttgg tcaccaagtt cgccgcgacc atcgatttct
      361 ttccacgcgc gccaacgtga ttccgacaat gatcaccttc gctgcgccga gccttcaatg
      421 cagagaatgt ttgtccgttc agtctcagtg gagatcgtta aattccggct aacttcaaaa
      481 attccgtaag attcgacggc gggaatttgt ggaagacccg tttggggaaa tcgccgatct
      541 ttatctatgt tgaagtagcg ccccgcattc gctcgcgttc gagagtcgag tccaaccggc
      601 caagtggagc aatctggttt tgcgagctgt cgggtatctc ttttgatcgc ccaaagcagt
      661 acggcacccg ctttagggtg gctctggcgc tccaggattg tgcgccacaa cttggtatct
      721 taaccaatga caaaagggcc agccatgcgc gtatctctcc gaggcgattc cgcgcggcgc
      781 ggctatgcga aatgcggcgc gatagcggtc gcgcccgcgc ccgcaagtca cccatctctt
      841 tgctcgcgcg gatttcgagc gcgatctcga actcgacccg gccgattcag agccgagcct
      901 cgccggcact gaagcaactg accagcggct catcgcccag ggcgacattc gcgatttcga
      961 agagcaggac gaaggctgcg acgatgtgct ggaatgacgc gaaatggcgc aagtcggccc
     1021 tggagggggg gtagaaaagt cgtatccctg gcctcgggcc tcgcgcgtat tgcctctctg
     1081 tcgacggtga cggccttcgg ccaagatttg cgaagggcga cgtcgtgatt gtcgaccccg
     1141 accgcatgcc gcagccgggc gacatggtgg tgctttacgg caacgccaag atcgccgcga
     1201 tcgcggagca cgacggccat cccaagcctg ttccatgcaa tcgtcggaaa ggtgtcccat
     1261 tcgctgtaat cggcgaacat gacgcgtccg ccgtccgctg gcgcttttcg aggcgcggtg
     1321 gtatgcagcc agtgggtgga ggggcacgca agggggcggc gaatgacttt cgctgaaatc
     1381 gttcttatcg tggctttggc gctcgcaatt ctcggcggct gggtcggata tagacgcgct
     1441 tttgcctctg gttacctgca tggatataac gccagtgcgt tgggcgatcc gccggagcct
     1501 cagtccgctg ccgtcaattc cctgcgccgc attttttgat gagcgggcat tcgccgcgct
     1561 gcgaggttcg ggactcgcga cggttttgat ttgcagcggc ccggaatcgc ccgactattg
     1621 ccattgccgc tagagcctcc aagctgcggc tagttcctca ggacgaagaa aaggcgccgg
     1681 tcccccggcg ccttttcgat tcttgggcca ctggatgctg aattccttgt aagacaaacc
     1741 ttacattttc atcgagctgc attgccatcc cattgatttt ttgtcatttc ttactgaggc
     1801 acaaatattg cgttgaaggg ctatacgagg cgttcacaac tacagggagg agttttatga
     1861 caaagacggt caatgctctc tatctcatca ccgcaatgat aatgatgagt ggcccggctt
     1921 ttgcgtccgc tgggtgctgc aatccaattc cggttcctga accgtcgagc cttactattt
     1981 tagcagcagg cttggccgca ggcggtattg gcgttttcct gcagaagcgc tttcgccgtt
     2041 caggccggct agtcgggctc ctttgtttcg cgacagcagc ggcgacgctc tgggcgtcca
     2101 tcggagtggc tggggcctgc tgctaacccc cttcgttggc ccgtgcggcg cgattcatag
     2161 agcaaagttt gtcttacccg ctcagccccg cttcggcggg gctttttttc gcatgtgcta
     2221 aaaaagtctc gcgtttcgta caatcgactc gcgacgattt tgatttgccg tcgcccggaa
     2281 tcggctgcgt atagcggttg ccgcgagcct cccatgctgc ggcgcggatg gtagccttag
     2341 gaaggaaagg cgctggctct ccccggcgcc tttctatttc gccgccttcc tgtcggtggc
     2401 ggcctggtat cgtttgtcgg catcgtgcgc tctagatcgc gggcgcaccc ggccgacgtg
     2461 cctattaaca catcgttgaa ctgagccggc gtggagtgca tccttgagcg ccttgatacg
     2521 cgccactgtc ggccgggatc ccggatgcgg tcgcgcagcg ccaaaatgac gatcaactca
     2581 ttgcgcgcgg cttaagtttc gccagccagc gggagaattt cggaccgtcg tcgaacacca
     2641 tcgaacggcg tctcatggga acgccgtttc ctaaaccatg aagtatgcgt gcgctttagg
     2701 ctacgcggcg acggcgaccc attgcactaa acccgacgag cgatgcaccg agcacagcca
     2761 gcgacgcagg ctccggcacg ctggtcgtac ctggagcaaa atccgtctga taattcggat
     2821 cgccgacatt cgagccagct agaacatagg cgcccggagc aagggaatcg tcatacacag
     2881 tgtaaaaccc gctcggaccg ccgctaaagg gaatgtggat cgacgcgatc tcactcgaga
     2941 gcgactcatc agagttcagt gagttggaag ctgcgagccc caactccaca ttgatcccgt
     3001 tcaacgcagg gccgttgttt ccgatttcct ccgtgatatc cagtgctaca ccgccccaag
     3061 tcaaggtcac cgcgacagca tcgctaatga cgagctgatc ccccgctgaa gaccgatgcg
     3121 ctgggacgcg ctgtcgctgc acacgttaac ttttctttgg tcttgcgtgc ataatatctc
     3181 catttatgag ggacgcatcc cgctgccctc ttttctggtc cgggtgagag cgccgattca
     3241 tgctttgggt cggcgctctc ttttggatcg acaaataacg ccgctggttg gtgatatgga
     3301 tgagtaagaa ctaagctaga ctatctctgg aatattgtcg gcgatttgtt ccatcagctt
     3361 tcgtctaaag tgtcgcaata cgaacacgcg gacagtcgag gtgaaattga aatcgcgctc
     3421 ctgctcgagc aatctgctga tcaaacgcga tcgcgtcgtt ccattttccg ccgcgatttc
     3481 atagagagct tgccagaact cgtgctcgag acacattgag acacgtcgct tttttccgtc
     3541 agcgtcggtc caacaaatgc atctgatcgg tagcttatgt tgcgcatcgc ttctcagtcg
     3601 agcgccgagt cttgtgccgt tcaaagttgc ggtttcccca atgacctttt cgcgaacatc
     3661 gatatgccca gcggtcttct atggcagtgc accttaattc aggtccttcc gatcactcgt
     3721 agcaatccag cttcatcaag atttttgccg gtgtcgtcat ccctgcaagc catcaacgaa
     3781 ggcgatatcg tagttgacaa ctagcgcaac ttatcgccca tcgtgtcgcc gatctaagcc
     3841 cattcctcaa aacgaccgat aaaaaaatct acaccctcga atgacaaaga gattttgggg
     3901 tttttacggg tgctgttgcc agtacggcgc taactgtctc ggtagtttca tcaggattgg
     3961 ttccaacctc cacccgactc tcgcgctgct cttctcggcg ataggaccgc gaggtcattc
     4021 ggcaaaatgc ccgtcactta tttgcgcact tccagaatgc gatggggcaa actgctttag
     4081 gcgggaagca aaacagagtc ggagacgaat tccttgagca cgcctgagtt ctggagcagc
     4141 gagtagatgt tgttcgggat gctccccgcg aggcaagctt tggatttgct cttcagtttc
     4201 aacgaccagc cttgcataat cttgtcaaat actatcggaa tgtcgctcga cgaagcgaga
     4261 taaccgcttt cggccagtgc ggcgcaaatc cgttctgcga tttcttcgta atccctgaat
     4321 gtgaggacca cattctggct ccgctcggcc gatccaatcg ctacgaactg aagtggttcg
     4381 ccggcggaga ctctgacgct caccggcgca atggcacggt caattacatc ttcaacatga
     4441 cgttgtgtga cagcaatagc catggtcgtc tctcgaacat tggtggatta cgagcgtcat
     4501 gatgacgatc aaggtcgctg acaggcctag agcgaaaaga aagaaacccc acatcttcat
     4561 cggatgatga tgataggggt gcaacaatga attttttctt gatgcttcga atgatcttgg
     4621 ccgcagccat cgcgccgtga cgagttgagc ctgacaagtt ccgacgacaa tagccaagcc
     4681 cccacccagg tgagacgtca ggtcagcatc gatttgtgct gtgtcgtcaa agatctttac
     4741 actcttgctg atacaatttt taagttattg attttttagt ttttttgttt ttggtataga
     4801 aattgcatct ttaagatgaa ataaaattta gaaaagcaac catcatgaag ggctaaaagt
     4861 caaacagcta tagttactag tcaaaaaaca ggaaacggcc caaccaattt ctgggtcttt
     4921 tgcgtgggag gatggaagat ggattttcgc ttgaacttgg ttccagcagg gatgttggct
     4981 gtcgccttat tcgctgttcc tgctatcgct gatacggtca cagatgacgg tgcgatgacc
     5041 catgggtgta ttatggctcg ggcaattcaa acaccgccat aacggttgac agcaacaatg
     5101 gggttcagct tggcgaaagc gcgatcacgc ggttcgtcgg gccgatcgcg ccgactccga
     5161 gcaacagcaa cgtttacgaa gttccgttgg gcgacacgat ggttgccggc aagacgggcg
     5221 ccaattgggg catctttttt ggcgtcgatc tcaccgggac tggtcttagt gtcaacgaca
     5281 tcaccgctac ctggactttg accgatcttg cgaggggcac tataggctct ttcaatccgt
     5341 tgctcattcc cgacaatgcg cttttgtttt cggacggctc cacatttaac ggcaacggta
     5401 ctcgaccgag cggcaaaggt ccgcccgttg gcgcagaaaa ttctgaagca ctttcgttcg
     5461 cgagcatcgc tggagctttc gatgacccat cattcagcga taacatcaac gacacctttt
     5521 atctgacttt gaccgtcgac cgggtgcccg catgtgccat atgttctccg acacaactcg
     5581 cctctgatga aatcgtgatc aaccagggta ctgcgattga tgaaccgtct tccatcgcaa
     5641 tcctcggcct aggattattg gcggccggat gtttcggcct ccttcgtatc aaaagaaaag
     5701 acggccttcc cgcatagcgt tacggttctg cggcgatacc tccttgggtt cattggcaaa
     5761 tttggctact tgagcccaag gagcttttct gctgaacaaa tccttgctcg atgccgtccg
     5821 gaaatggacg gaagcgaatg cgtttgtcgg acggtgacac agccactgag attggcgcga
     5881 tcgggcgtcg tcgaactgag atgccgaagc gacatattct ggttgtgtga accaaaccgg
     5941 atcaactaac ccttcctgtg atcgagtgta gagagatttg gaatctctac aattccgcac
     6001 cgaatctcgc tcctcgagcc cgcgcgcgaa attggtgtct cattagctcg atcaaattgc
     6061 tatattggaa actggtcatg ttatgtggcc aatgtgctat ttttaagaga atggcaagtg
     6121 ggttattcgc cctgccgttg gttttatgca attgcagcga ttggaacgtt caagtgagtt
     6181 cgtcgcaaag aaccattcag tcagatggtg agcggctatc ccgaatccga tcggagctgc
     6241 cgccggctaa agaggatttc catcccggcc gaaaactggc tgcggtgccg cgcgtggacg
     6301 agaggtccac ggaatggacg ccagtccgtc gggccccgcg tctcctcacg agagaagatg
     6361 cggcgcatta ttgcgcagtt tccacgcacc aatttatgcg acatgtcgcg ccgcatgtcc
     6421 gagctcttag aattggaaat cgtctccgtt gggacatcaa gatgcttgac gcatttctgg
     6481 acgtgtcagg ggggcaggtc gtccaaccgc cgctgtcgat ttctcagtta ttaggagtac
     6541 ctgccagatg aactttcgaa ttcgaggcgt gaaaaaggtc cgggcaaagg gacgagtcta
     6601 ttattaccac cgaaagacaa tgatccgact gccgggcctg cctggatcga cagcgtttat
     6661 ggatgcaatt gcgcgggctg agcgaaacaa aattgaaccg gacgtattac ccggaacact
     6721 cggagctatc atcagtgcct accgtgcctc gccagaattc agaagcctca gggaacggac
     6781 gcaacgcgac taccaaaacg ttctgaactt cttggcgcct ttaatgcagt tgcctttgac
     6841 ggaagttggt ccgcgccaga tatacgaaat tcgtgacgcg gcttttgaac ggaagaaacg
     6901 gagattcgcc aattatgtgc ttcaggtatt gagccgcata ttcaactggg gaaagtcacg
     6961 agggttctat gagggaaacc ctgcggaagg cgtcgaacgg gtgcggcgac cgagggattc
     7021 gaaaacagtc aataggcgct ggactgacgc ggaatttgag attgtgctga ccaacgccaa
     7081 ggggggcttg aggcttggaa ttgccttggg cggatatctc ggatttcgat tgggcgacgt
     7141 cctttctctg cagtggaatg cttatcagga cggtgcgttt gaaattaggc agcgaaagac
     7201 gggggagagc ctttgggtac cgtgcacaat gatctaaagt cgcttctcgc taatcaaatt
     7261 gaacatcgca atagtacgtt gctcgtctcg acggcgaacg gtcgtccgta caaaattcag
     7321 ggctttcaga gcatgttctt ccgactcatc cgctcgctgg ttgcccggca gttggttcag
     7381 cccggcctat cgtttcacgg gctgaggcat acggtaggca ctaaattagc ggaagccggt
     7441 tgcgacgcac ggacgatcgc cgcgatgctc ggtcattcca caacgcacat ggcggaacac
     7501 tactctcgga atgccgatcg caaggagctc gtgagagaag cagtgcagcg gctcgagcaa
     7561 cgctcgcgga acgcttgatt tcaagtcgac ggttacgttc agatcaacgc cacgattctc
     7621 gctgacgtcg gcaatcgttg tcggtcgcct ttcgccggta gacccgggac gaacggaatg
     7681 gattcaagac tcggacgcat cgtgagttgc gcgccctctt ttttgaattg cgcccgcgtt
     7741 aaccattttg gcagcgaatt aacctatcga ccgtggaaag acagcgcctt taggctcttt
     7801 cctatattct acccgaaagc atgcgaggtt tttgtgggta agccgctagc cgccgtttat
     7861 atatcgccag tttccggcat gccgaaaatc gcggcgattg tccaggacgg tcaggttact
     7921 tacgctcaaa cagtcgcgga tgaggtggag ggcaaggtcc tgttgagtga catgctcgct
     7981 atcttagacc cgccaaacgc gccaaagacc aaagggccgc gctgagcatg cctaaaggcc
     8041 tccaaggtca aagcgtcccg acgacgtgat cgccaccgtc aggggttatg tagatcgagc
     8101 cgacaatcca ctcctcgcac ccgtacccgg tgatttccta gtgagttttc ggaccaagga
     8161 gccaaggccc ggcttgctgg agcggatcat ttggcccggc tctcgcccta ctggcggaac
     8221 aaccgctaaa tagcgatgca gcacgcttcg ctcgctgacg atcatcgcga cagttcttgt
     8281 gcagcgacag ttcttgtgca tttcagctag cgcccaaaca tctcgcaccg catatcaatg
     8341 gactacgatc gcggacacga tgcgtgaccg ctgccgcacg accacgtggc ttaatcttgt
     8401 ttctctcaat ggcgggaagc ggggcaacgt tgacggatta ccgctgctcc atatcggtat
     8461 gctcttcttc aagcgggcag tcatcggtgc cgtgaatctg aacgagacct gttattttgt
     8521 cttctatgaa atgttaacgt gggtttaatt acataccctt atctcgagtg ggcgcaatgc
     8581 cgacggctta tcggctgtga ggtggatcgc gccgttgcgc gaagtcgaga ttgggagaca
     8641 ggtagtgacc acccgcgcgt tatttcgctt caccggacct gaaaaatccc aagtcgcctg
     8701 tatcgacaaa ggaaatgcct gggcggtgca gagtcagacc gaattccttt ctgcgaacgg
     8761 cgggaataca acagttgctt ggctatggga tgctcttccc tatgtgccga agccgatgga
     8821 cgattatccc gaattggaag ccgctctcga gaaggttcgt cattatctga cggaaatgca
     8881 gcccgttcgc gagaaggtgg cgctgcacaa aagccaagga attctagccg acgagacgga
     8941 gttgctcgga aaaattgttg aggctcacga acgcctcata tcgatgtatt tggcgaaggc
     9001 ggttaagttg tttgaggcgc gacgcccgct cgcatcgcag aagaacgctg tcggttcgct
     9061 gacgagaggg cagaagggcg gcgcaactct tgccgtaaga aatgacgacc ttccagtcga
     9121 aactccgtcc agtttggaag acggatcgac tcctcggccc gagcgaatgg ctctcaagag
     9181 taaagaattg aaagacggat gcgtggctct gattacgaag attctcaacc aaacgctgcc
     9241 ggaggattcg ggtgcgcacc ttccctcgac tgctatcgaa gatctaactc aacagatgat
     9301 tcggacggtt ttcgaaccta ggggccggct acgcgagcga gcgcggcggg accccagaag
     9361 aatgactgaa attgttgggc ttgcgacgtt gcccatcgtg acgcgaggca accgcgataa
     9421 cgtcgcaccg accctggttg acatgttgga acacgcgaat ttggtctcgt tcgacgttga
     9481 gctcagcgac gcagacaatt ggccacggct caaagcgcgc gcgcagcgtg gattgactgt
     9541 cgcctgacgg taatcggctt cgtcgacgat cggctttcat ttgaatgcgt cgagcgtatg
     9601 gaagatcggt gagaccgcag aggcggtgtt gcttgctata tctgggacac gcgcgccctc
     9661 cctagctttt gtgtgcgagc gcctgcaatg tttctctgcg atcgacgctc gtggcagtgc
     9721 cctagccgac gatctggagg cctcggaccg gttcggacat tgcgtgttgc agcttctcag
     9781 aggcacttct accttcagaa tttgaaagtg gaaccaggca tacacgatgg tcatcgaacc
     9841 ttcggctaga tggtttgcct agcccgccca caagaatttc ggatggctcg ctgcagacaa
     9901 ccctagcgac gaaaagctcg tgggctaggg atcaggacga tgcgactgcg ttaagacttt
     9961 tcttgttgcc gattttgccg aagcggtcgc acacccttca accatgatac tgatctctga
    10021 aaatccgccc cgcattttag tagcggtgcc tcagcgagtt tggaacggca tgcgttgcgc
    10081 gaccataatc tccagcgcat tgtggggggt gtttgcctca cttgttgcgg ggtatggctg
    10141 atggccgcgc gcgacaaggt tcgttcttcc cgggtcgtct atggtccgcc gcgggcggga
    10201 ctgccatttc tgtcagtgat ttttatttct gatcgcgacg aaccgatggt aacggcattc
    10261 gcaagcgcgg ccgaggcgga ggaatttaac gagacgatcg ataggttccg tcccg
//
LOCUS       DATNDT010000408         8156 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_264050, whole genome shotgun sequence.
ACCESSION   DATNDT010000408 DATNDT010000000
VERSION     DATNDT010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264050"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..675)
                     /locus_tag="VN788_13275"
     CDS             complement(<1..675)
                     /locus_tag="VN788_13275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003618587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HXU21548.1"
                     /translation="MSTYVLVHGAWHGSWCWKRVRKALQAQGHDVFTPTLTGIGERSH
                     LLSPNVNLGTHIDDVVNLIRWEELSNVVLCGHSYGGAVISGVADRIPERIGALIYLDA
                     FLLENGRSVHDELPPEQRNMQLEDARVHGEGWKVPPIPAAGFNVNAQDAAWVDHQCTM
                     QPLAAFQQPLRLSGKMDTVKDVTFVLATGYGGSPFMPSYERAKAKGWKMLSMACGHDV
                     MLDLPEE"
     gene            817..1146
                     /locus_tag="VN788_13280"
     CDS             817..1146
                     /locus_tag="VN788_13280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21549.1"
                     /translation="MDTQATDLQLLANRIAKLERQNRFWEIGGVLAALALASLLAVGV
                     RAQQEWLRATQRAKLIEAQDFVLTDANGITKGEFSTKDGNPILELYGPTGKVIWSTNP
                     RMVIQGQ"
     gene            complement(1180..3276)
                     /locus_tag="VN788_13285"
     CDS             complement(1180..3276)
                     /locus_tag="VN788_13285"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HXU21550.1"
                     /translation="MKVLVRLLTFLAFASPAFAAHSFHVEDMQKLSRVAAPKVSPDGK
                     WVAFTVTRSDVAKNKTVTNIWMVAATGDKPRQMTFADQGSNNSPRWSPDGRYLYFLST
                     RLENKPQIFRLPVAGGEAEQITKVPTGVGGYVLSPDGKTIAITASVFPSCSDMACNEK
                     KAKERADNPVKVRVITEMPFRRWDTWVDGMRNHIFIMPADGGAAKDITPGDVDSPTWQ
                     EEGSEEVAFSPDSREICFSRFVENESLTGNSDLFVMPASGGTPKPITTNKSTDRTPLY
                     SPDGRYIAYSATLRPMQETDLVRLFLYDRKTGEHKNIFEAADRSVDSYIWSPDSSALY
                     VTFEDKGSEPIERLDVATMKLTPLVTGGSSGEEDISRDGAFLVFSKNDISHPADVFRL
                     NLPAGTGVAPSQLTQMNADILKDIEWGEVSSFTFPGWNGEAVQAWEIKPPEFDAHKKY
                     PLLLLMHGGPENAWDNLFHYRWNLQLFAAAGYVVIAPNFHGSSGFGLKFMDSIKGQWG
                     GAPYEDQMKAVDTALTWPYVDTTRTAAAGASYGGYMANWVEGHTDRFRAIVSHDGLYD
                     LVTMLYSGDFVGGALPEFKGTPWENPQALIDQAPATYAKNFKTPMLIVHGGHDYRVPQ
                     SQGFAMFQVLQAEHIPSKLLYFEDENHWVLKPANDILWYHTVLDWLDQWVKPDRAEYE
                     KQLKAASAEMRSGQHE"
     gene            3687..3878
                     /locus_tag="VN788_13290"
     CDS             3687..3878
                     /locus_tag="VN788_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21551.1"
                     /translation="MDPAFDIFKLLPDGPLRVMAVQGLEQANEQMARLVQTSPGEYFI
                     RSHQQMVELEQPEEWAEVT"
     gene            3994..4398
                     /locus_tag="VN788_13295"
     CDS             3994..4398
                     /locus_tag="VN788_13295"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU21552.1"
                     /translation="MSAEAIMSQGILIADDSRNVRQAIHNYLAERNFHVCGEAVDGQD
                     VIQKARELKPDLVLMDLAMPGTNGIVAASVVKHMIPNVRIILFTMYSEAVSKAFTSLK
                     DVDAMVDKSDGMDKLAECVQGLLGPSDETANN"
     gene            4621..6453
                     /gene="bshC"
                     /locus_tag="VN788_13300"
     CDS             4621..6453
                     /gene="bshC"
                     /locus_tag="VN788_13300"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HXU21553.1"
                     /translation="MNSSMESHCLSFREIPHTTKLFSTFLEDFPRVASFYAHPPTLAG
                     IDAAAREVRLDSNIRRQVCEILREQNQKFNVGRELDAATNRNLDRLARGAVAIVTGQQ
                     TGLFSGPAYTIYKAVSAIRIAEEATSGDTDAVPIFWLATEDHDLAEVHHSDWNTREGL
                     VHYELPARTEDAGRRVGEIPLGDEIEAIVVAATQALEGPFAEEVARALRESYTRGETY
                     GSAFGKLMARLFAGRGIIFIDPLDARLHRLAAPIYLRALDEADSLGDALLARSNELES
                     AGFHAQVKVTRETTLLFYNVDGRREPVRRRNGNFAAGDAEFSRQQLTSAFKDRPDAFT
                     PSALLRPIVQDSLLPTAAYIGGPAEIAYLAQSNLIYKQFIGRMPAILPRASFTVVEPP
                     IARFLEQYGLNFRDILAGPQHLRAKMEQKFMPGDLANRFDAGETALRDLLAQYGEPLK
                     SLDPTLVDALRASEQKMLHQFTQLKAKVARAINFRSGVLDRHQRILVDALHPAGGLQE
                     RTLSALPFLAAHSSQLLDDLAALSSTSDPAIASSPNVPVPTHAAPSISMVASISDATS
                     AKAAATTAPSSMPAKTQAPSPGVSIADAFAARQLCAKAHYVIHL"
     gene            complement(6946..7695)
                     /locus_tag="VN788_13305"
     CDS             complement(6946..7695)
                     /locus_tag="VN788_13305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21554.1"
                     /translation="MTRRTLEITALCIALLIAALATHAWLSSRDEQQRFQATLTTEKK
                     ILDAANTRERTRDATLNDALAKIEKLKRATQTPQQILSDLPNYLPLPQPIALARPAAD
                     THPQQGTALPAKSDAAALPSPSEPGYPAHSAEPRTSSPTSTTTLPPPAQPAIPCNPGE
                     NCGLEMPSADLKPLYDFTQSCRECQLRLAAAQQDHSDDLTKLDSLTRERDAAITAAKG
                     GTFWRRLRRNALWFVVGAAVGAATAQIAAHR"
BASE COUNT         1648 a         2402 c         2313 g         1793 t
ORIGIN      
        1 ttcttcgggc aaatccagca taacgtcgtg gccgcaggcc atgctgagca tcttccagcc
       61 tttcgccttt gcgcgctcgt aagagggcat gaatggcgat ccgccgtacc cagtcgccag
      121 gacgaacgtc acatctttca cagtgtccat ttttccgctt agccgcagcg gttgctggaa
      181 agccgctaac ggctgcatcg tacactggtg gtctacccac gcggcatctt gggcgttcac
      241 gttgaaacct gcggctggga tggggggcac tttccacccc tcgccgtgga ctcttgcatc
      301 ctccagttgc atgttccttt gttctggcgg cagctcatcg tgcacgcttc ggccgttctc
      361 aagaaggaac gcatccagat aaatcagcgc gccaatccgc tccggtatgc ggtcggccac
      421 tccgctgatc actgctccgc catacgagtg gccgcacagc accacattcg aaagctcctc
      481 ccagcgaatc aagttcacca catcgtcgat gtgcgttccc agattgacgt tcggagaaag
      541 caagtgcgat cgctctccga ttcctgtcag cgttggcgtg aacacatcgt gcccttgcgc
      601 ttgcagcgcc ttgcggacgc gcttccagca ccaactgccg tgccacgcgc catgaaccag
      661 gacatacgtg ctcatcgctt acctcgccca caaatcgccg ccatcatgcc atagccacct
      721 accttcggcg acgcgaaatg ttccaggctt cctttcccgc gtaccgcgcg tctacatttt
      781 cgagggagaa accaatcagc catgaggagg cgagccatgg acactcaggc cacggatttg
      841 cagcttttgg cgaaccgcat cgcgaaactc gaacggcaaa atcgtttctg ggaaatcggc
      901 ggcgtgctgg cagcgctggc gctggcgtcg ctgttggctg ttggcgttcg agcgcagcag
      961 gaatggctcc gcgccaccca gcgtgccaag ctaatcgaag ctcaggactt cgttctcacg
     1021 gatgccaacg gaataaccaa gggcgagttc agcaccaaag acggcaatcc catcctggaa
     1081 ctgtacggcc cgaccgggaa ggttatttgg tccacaaatc ccaggatggt aattcaaggc
     1141 cagtagcggc accgcgcaat accttggttc gtcgggcgac tattcgtgct gccccgagcg
     1201 catttctgcc gacgccgctt tgagctgctt ttcgtactcc gcacgatcgg gctttaccca
     1261 ttgatccagc caatccaaaa ccgtgtgata ccacagaatg tcgttcgccg gtttgagcac
     1321 ccagtggttt tcgtcctcga agtagagcaa cttggatgga atatgctccg cctgaagcac
     1381 ttggaacatt gcgaaaccct ggctctgcgg gacgcgatag tcatgaccgc catgaacaat
     1441 caacatcggc gttttgaaat tcttcgcgta cgtagccggc gcctgatcga tcaaagcctg
     1501 cggattttcc cacggtgtgc ctttgaactc gggcagcgcc ccgcccacaa aatcgccgga
     1561 gtagagcatg gtcacgagat catataaacc atcgtgggac acgatggccc ggaagcgatc
     1621 cgtgtgcccc tcgacccaat tcgccatgta accaccgtag ctcgcgcccg ccgcggcagt
     1681 tcgcgtggta tccacatatg gccaagtgag cgccgtgtcg accgccttca tttgatcctc
     1741 gtatggtgct ccgccccatt gccccttgat cgagtccatg aatttcaggc cgaaccctga
     1801 tgagccgtga aaattcggcg cgatcaccac atagcccgct gccgcgaata attgcaggtt
     1861 ccatcgataa tgaaagagat tgtcccaggc gttttccggt cctccgtgca tcaggagtag
     1921 caacgggtat ttcttgtgcg cgtcgaactc gggaggtttg atctcccacg cttgcacagc
     1981 ttcgccgttc catcccggaa acgtgaagct ggagacttcg ccccattcga tgtccttcag
     2041 gatgtctgca ttcatctgcg tcaactggga gggtgctacg ccagtgcctg ccggaagatt
     2101 gagccggaac acatccgccg gatgagaaat atcatttttg gaaaagacga gaaaggcgcc
     2161 gtctctcgag atgtcctctt cgcccgaact gccgcccgtg accagaggtg tcagcttcat
     2221 tgtggccaca tcgagtcgct caatcggctc ggaccctttg tcctcgaacg tgacgtaaag
     2281 ggcgctacta tccggtgacc atatatacga atccacggac cggtctgcgg cctcaaaaat
     2341 attcttgtgt tctccggttt tgcggtcgta gagaaacagc cgcaccaaat ccgtttcctg
     2401 catcggccgc agcgtggcgc tgtatgcgat atagcgcccg tccggcgaat agagcggcgt
     2461 tcggtccgtg gatttgttcg ttgtgatcgg tttcggagtg ccgccacttg ccggcattac
     2521 gaacaggtcg ctgtttcccg tcaaagactc gttttcgaca aatcgcgaaa aacaaatttc
     2581 gcggctgtcg ggagaaaatg caacctcctc gctgccttct tcctgccatg tcggggaatc
     2641 cacgtcacca ggcgtgatat cttttgccgc tccgccatca gccggcataa tgaaaatatg
     2701 attgcgcatg ccgtcgaccc aggtatccca tcgccggaac ggcatttcgg tgatcacgcg
     2761 cactttcacc ggattgtccg ctcgctcctt ggcctttttc tcgttgcatg ccatgtcact
     2821 acaggaaggg aacacgctcg cggtgatggc gatcgttttc ccatccggag agagcacata
     2881 acctccgacg cccgtgggaa cctttgtgat ctgctccgct tcgccgccgg cgacaggtag
     2941 ccgaaaaatc tgcggcttgt tctccaagcg tgtgctgagg aaatatagat aacgtccatc
     3001 cggagaccag cgggggctat tgttcgaccc ctgatccgcg aaggtcatct gtcgcggctt
     3061 gtcacccgtc gcagccacca tccagatgtt cgtcacggtt ttattcttcg ccacatcgct
     3121 ccgcgtcacg gtaaacgcaa cccatttccc atccggcgac acctttggcg ccgcgacgcg
     3181 cgacagcttc tgcatatctt ccacgtggaa tgaatgtgcc gcgaatgccg gcgaagcgaa
     3241 agccagaaac gtcagcaagc ggacgagaac cttcatgttt cctcgcaatc tacaaacttc
     3301 tgcgtggatg actattcagc cagcagctgt tttgtcagcc gatcttaaat caaacgcgtc
     3361 acaccggcac accctcgggg atcgccgtgc tttggccaac attgagccat ggtgagcgcc
     3421 gaaccccccg tgatggtgaa cgcccgagtt ctcggcaata tacacgcccc gccgtccttg
     3481 tcaacgcgga gtcagcgacc gccggtcggc tcacagggga gttccggtac gacagacagc
     3541 aatatcaccg acaaaaagtg cccgctgtca ccacccgcat tcgagctcca agcgcgttca
     3601 aagggcctca agtccttgat attcctgatc taacttattc cagtgccttg gaaccgaaag
     3661 tgctcaccgt aactgcggag gccaatatgg atcccgcctt tgatatcttc aagctactac
     3721 ctgacggtcc tctgcgtgta atggcggtgc aaggtctaga acaagccaac gagcaaatgg
     3781 ctcgcttggt tcagacttcg cccggggaat acttcattcg ttcacaccaa caaatggtcg
     3841 agctcgagca gcctgaagag tgggccgagg tcacctgacc aactccgctc tcgtctcaaa
     3901 aggggaaacg attcacttgt ttccgaggct agggcaaaca ccctatatcc atctcctcag
     3961 gccactgcta ctgttccttc gaaggagatg gcgatgtcag cggaggcgat catgtcccag
     4021 ggaattctca tcgcggacga cagtcgcaac gttcgccagg ccattcataa ttatctagcc
     4081 gagagaaatt ttcatgtgtg cggtgaagcg gtcgatggcc aggatgtgat ccaaaaagcc
     4141 cgagaactca agcctgactt ggttcttatg gatttggcga tgcccgggac aaacggtatc
     4201 gttgccgctt ccgtcgttaa acatatgatt cccaacgtgc gtatcattct gtttacgatg
     4261 tatagcgaag cggtaagcaa ggcgttcact tcgctaaaag atgtcgacgc aatggtcgat
     4321 aaatctgacg gcatggacaa attagcggag tgtgttcaag gtttgctcgg cccttccgat
     4381 gaaacggcaa acaattagtt agctttttct tttcagatct cgtgtgccca cgctcttcgg
     4441 ttcacatagc tcttcggctt catctagctc accccgactt caaggagtga ggcttttcgc
     4501 cgtcgttccg tcccgcggat cgaagggccg ctgagcttcc cggttattgc agcctggcgt
     4561 accagaaact acccggtacc gcgagcctca aagttgccgc tcttcggagc cccgtgtatc
     4621 atgaattctt ccatggagtc ccactgtctt tccttccgcg aaatcccgca cacgactaaa
     4681 ctcttttcaa cgttccttga agatttcccc cgagtcgcgt ccttctacgc acaccctcca
     4741 acactcgctg gaattgatgc cgcagcgcgc gaagtccgcc tcgattcaaa tatccgccgc
     4801 caggtttgcg aaattctgcg cgagcagaac cagaaattca acgtcggcag ggaactcgac
     4861 gctgcgacaa atcgcaacct cgaccgtctt gctcgcgggg ccgtggcaat tgtcaccggc
     4921 cagcaaaccg gccttttttc cggtccagcc tacacgattt ataaagccgt ttccgcaatc
     4981 cgcatagccg aggaggcaac tagcggcgac actgatgcag ttcccatctt ctggctggcg
     5041 acagaggacc acgatcttgc ggaagtccat cattccgatt ggaacactcg tgagggactc
     5101 gttcattatg aattgcctgc gagaactgaa gacgcaggcc ggcgcgtcgg agaaattccg
     5161 ctcggggacg agatcgaagc aatcgtagtt gcggcgacgc aggctctcga aggccctttc
     5221 gctgaggagg ttgctcgggc gctccgcgaa tcttatacgc gaggcgaaac ctacggctca
     5281 gcctttggca aattgatggc gcgactgttt gccggtcggg gcatcatatt tatcgatcca
     5341 ctcgatgcgc gcttgcaccg tctggctgcg ccaatttacc ttcgcgcgct ggacgaagct
     5401 gattcgctcg gcgatgctct gctggcccga tcaaacgagc ttgaatccgc cggctttcac
     5461 gcgcaggtaa aggtcacgcg tgaaacgacg ttgctgtttt acaacgtcga tggccgtcgc
     5521 gagcctgtgc gccgccgtaa cgggaatttt gccgccggag acgcggagtt ttcgcgtcaa
     5581 caattaactt ctgcattcaa ggatcgccca gacgcattca ctcccagcgc gttgttgcgt
     5641 cccatcgtgc aggattcgct tcttcccacc gccgcataca taggaggccc ggccgaaatc
     5701 gcctacctcg cccagtccaa cctcatctac aagcaattta tcggacgcat gcccgcgatt
     5761 cttccgcgcg ccagtttcac agtcgtcgaa ccccccatcg cccgttttct ggagcaatat
     5821 gggctcaatt tccgcgatat cctcgccggg ccgcagcacc ttcgcgcgaa aatggaacaa
     5881 aaattcatgc ccggtgattt agccaatcgc ttcgacgccg gcgaaaccgc tttgcgcgat
     5941 ttactggccc aatatggcga gccgctgaag agtttggatc ccaccctagt cgatgccctt
     6001 cgcgcctccg agcagaagat gcttcaccag ttcacgcaac taaaagctaa ggttgcccgc
     6061 gctatcaatt ttcgctccgg agttctcgat cgccaccagc gcattctcgt cgacgccctc
     6121 catcccgctg gcggattgca ggagcgcacc ctaagcgccc taccattctt agcagcccac
     6181 agctcccaac tcctcgacga cctagccgct ctctcgtcca catccgatcc agcaatcgcc
     6241 tcctcgccca acgttccggt gccaacccac gccgctccgt caataagtat ggttgcctcg
     6301 atcagcgatg ccacctctgc taaggccgcc gccacaactg cgccctctag catgcccgca
     6361 aaaacccagg cccccagccc cggagtgtca atcgcagacg cgtttgcagc tcgccaactc
     6421 tgtgccaagg ctcactacgt catccatcta taacgccgtc aaaatagaag gccaacaccg
     6481 ccccacgtgt aacgtaacat aaccagcgcg agcaccgcca agacgaacgc cttcggccca
     6541 atgcaacata gtaagaatta gcggaagtca agggtatgta cgttttacgc tgtggattgt
     6601 ggagggcggc gtggcggtcc gggggaaagg gcaaatgtga gcaagaggcg aaacaggagg
     6661 aggggggagc cggaacaggg aaaaggcgga acgaggtcat aggtgccctc cctccaatcc
     6721 cgggcgcccc ccgcgcttcc tgcgataggc tcgatcggga ttctcagcgg taagctcgat
     6781 tgggattgtc ggcgcgagct cgattgggat tgtcggcggt aaagtcgatc aggattgtca
     6841 ggcttgtcga ttgggattgt cagcgaccag gttatcgcct gggttgcagg gtattgcggg
     6901 ctggggcggc agagttccgg tcagcgatta ggtcgattgg gattttcagc ggtgtgcggc
     6961 gatttgggcg gtggctgctc ctacagctgc gcccacgacg aaccagaggg cattgcgacg
     7021 gaggcggcgc cagaaagtgc cgcctttggc ggcggtgatg gctgcgtcgc gttcgcgggt
     7081 aagggaatcg agcttggtga ggtcatcgga gtggtcctgc tgggcggcgg cgaggcgcaa
     7141 ctggcattcg cggcaactct gggtgaaatc gtagaggggt ttcaggtcgg cggagggcat
     7201 ttcgagcccg caattctcgc ctgggttgca gggtattgcg ggctgggccg gaggcggcag
     7261 agtcgtggtg cttgttggcg aggaggttct gggctcggcg ctatgggcag ggtagcctgg
     7321 ttcgctgggg gatggcagcg cggcagcgtc ggatttcgcg ggcagggcag ttccctgctg
     7381 aggatgggtg tcggcggccg ggcgcgcgag ggcgataggc tgagggagcg gtaaatagtt
     7441 ggggaggtcg ctgaggattt gctgtggcgt ttgcgtggcg cgttttagct tttcgatttt
     7501 tgcgagtgcg tcattcaggg tggcgtcgcg ggtgcgctct ctcgtgttgg cggcgtcgag
     7561 gatcttcttt tcggtggtca aggtggcttg gaagcgctgt tgttcgtcgc gagaggagag
     7621 ccaggcgtgg gtggcgagcg cggcgattag caaggctatg cagagggctg tgatttcgag
     7681 ggtgcgtcgg gtcatgggag tttgtggcga gtggctggtg gccggtgaat taccgactga
     7741 cgaggatggc aatttgtacg tggtgccttc cagcgggaaa tgcgactaca tgctgcgaga
     7801 gagcagcgca atggatcgcg cttcgcgcga gccgtcttgt gtcaggttga agccctgacc
     7861 gccggtcgtg ccatgacgag agcgcggttg aaattggtgg ccttcgcagg cttgaatgtt
     7921 cacgcttgcg agtgactagc ggctagtgac caatgatttt cagctggcgg ggatggcgat
     7981 tggctgctgt tgcctgtaag tgggggaatt cgagtacgtg ccgcgagaga ggcagcgcag
     8041 tgggaagcga ttcgcgcgcc gtcgtttatt gcgcgggcct aaaggcctgc gcttccaccc
     8101 gggccgcgct tcgcgccgtc gtttgtgtgg cgggaatgaa tcccccgccc ttccgc
//
LOCUS       DATNDT010000409         4450 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2673092, whole genome shotgun sequence.
ACCESSION   DATNDT010000409 DATNDT010000000
VERSION     DATNDT010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2673092"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1884)
                     /locus_tag="VN788_13310"
     CDS             complement(<1..1884)
                     /locus_tag="VN788_13310"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXU21555.1"
                     /translation="MGTIPAMATSNLRLSMLQRGTRGCAIRFACSLWAFFLLSTITGA
                     QVARQNPVSGPELARHNLAQVGASAEDIKAVLVKSPGLMVEVKRWVAKDATDQGQIIT
                     ERDLAQDAIFERLQNDVRFRSVVTQLLQRYGYLMPAVNPDSQAGREQDLLVQERVKFL
                     AQEEEQQRAQALQQAQDRYENARKCSQGDTKSCEVAGQQRQGQIPQELAPQPQGTVPG
                     GPPWLNPNQSSPFVQPPAGGSNTLEQAQLMQTGQFSDDQLSPPYMPSSMDSSGGAGGD
                     TASLLQRMQLDENGGESTSASPGSSGLTASPFDSHSSDADLGASFDRTDINPFDATQQ
                     GQTGGAAMNAPIGALRDQRNQMQPAAMVRKPSPYKDIPSLYDMYMQATPRPAATTRFG
                     MDVFQNNSQNPYAIPMDLPAGPDYVVGPGDGLAIDLWGGVSRRIYRSVDREGRISLPE
                     VGPLLVSGKSLAEVQQNLQQVLRTQFRNISADVSLARLRTVRIYVVGDVANPGAYDVS
                     SLSTPLNALFIAGGPTREGSLRIVRHLRGGQLVQNVDLYDLLLHGVTSDIQRLQNGDS
                     VLVPPIGSQVTVEGMVRRPATYELKDEKSLSSVLELAGGLLPTATLRHIEVQRTIAHQ
                     KQTM"
     gene            2198..2758
                     /gene="efp"
                     /locus_tag="VN788_13315"
     CDS             2198..2758
                     /gene="efp"
                     /locus_tag="VN788_13315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HXU21556.1"
                     /translation="MIKATLLRPGMIIKHEGDLHVIYSVDHRTPGNKRAAMATKMRNL
                     RNGSMLDHRFRAEEFLDKVTVDEIEFDYLYSDGEGHHFMNTQNYEQLALQNDVVGDSK
                     DYLIPNMPVKIEYYDEKPIGVLIPDTVDLMVVETEPSIQKATASSVMKPAKLETGLVI
                     NVPPFVGTGDKVKVDTTEGRYIQRVQ"
     gene            complement(2784..4103)
                     /gene="purB"
                     /locus_tag="VN788_13320"
     CDS             complement(2784..4103)
                     /gene="purB"
                     /locus_tag="VN788_13320"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HXU21557.1"
                     /translation="MIARYTRSEMGRVWSDENKFQRWLDVELAASETLAEAGIVPREA
                     AAKLRARARINVARINEIEAKVRHDVIAFTIAVQETVGDPEAARWLHYGLTSNDVVDT
                     AQALLVRDASNLIEKGIARFGEILEKRAWEFKDTPEIGRTHGIHAEPITFGLKIANWY
                     AENRRDATRFETAAREMAVGKISGAVGTCTHLGAEIEQKICQRLGLSTAPISSQVIER
                     DRHAQYLSALAIVAASLERVALEIRHLQRTEVREVEEPFGGEQRGSSAMPHKRNPVSS
                     EQICGLARVVRSNSLAAMENVALWHERDISHSSVERVILPDSTTLVDYMLHRTSEIVA
                     NMKVFPERMRRNLDATKGLVFSGQLLQDLVEHGAPREDAYKWVQSHAMAAWESETSFR
                     ERIVADQNIRKHLDEKALAHTFDLQRQLRAVDAIFTRVFGAKSSAAR"
     gene            complement(4261..>4450)
                     /locus_tag="VN788_13325"
     CDS             complement(4261..>4450)
                     /locus_tag="VN788_13325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21558.1"
                     /translation="PFLPPVGSRIQLLHARSQEVREFRVISAKGPKVPGSFELGIEIL
                     HPAQNFWGVQLPEDRCSA"
BASE COUNT          926 a         1309 c         1244 g          971 t
ORIGIN      
        1 catcgtttgt ttctggtgag caatggttcg ctgcacttca atatgccgca acgtagctgt
       61 tggaagcagg cccccggcca gttccaggac actggacaag ctcttctcgt ccttcaattc
      121 ataggtcgcc gggcgccgaa ccattccttc aacggtcact tgcgaaccaa tcggagggac
      181 caaaacacta tcgccgtttt gcaggcgctg aatatccgac gtcaccccgt gcagaagcaa
      241 atcgtacaag tccacgtttt gaaccagttg gccgccgcgg aggtgtcgga caatccggag
      301 agatccctct cgcgtgggtc cacccgcaat aaacaacgcg tttagcgggg tcgagagcga
      361 actcacatca taggcgcccg gatttgcgac gtcgcccacc acgtagatcc gaacggttct
      421 aagacgcgca agcgacacgt cggccgagat attgcggaat tgcgtgcgca ggacctgctg
      481 caaattctgc tgaacctcgg caagactctt cccgctcacc agtagcgggc ctacctcagg
      541 caagctgatt cggccttcgc ggtcgacact tctatatatt cggcgcgaca cgcctcccca
      601 cagatcgatt gccaagccat ctcccggccc gaccacgtaa tccggccctg ccggtagatc
      661 catcggaatc gcataaggat tctgtgagtt gttttgaaat acatccatgc caaaccgcgt
      721 cgtagctgcg ggccggggcg tcgcttgcat gtacatgtca taaagcgaag gaatatcctt
      781 gtatgggctc ggtttgcgga ccatcgccgc cggctgcatc tgattccgct gatccctcaa
      841 cgcaccgatc ggtgcgttca tggcggcacc gcctgtttga ccttgctgtg tcgcgtcaaa
      901 aggatttatg tccgttcgat caaacgaagc tccgagatct gcatcgctcg agtgggagtc
      961 gaatgggctg gctgtcagtc cggaactgcc cggagaagca ctcgttgact ctccgccatt
     1021 ctcgtcgagt tgcatccgct gtaggaggga cgctgtgtcc ccgcctgctc cgcccgatga
     1081 gtccatcgag ctgggcatgt aaggcggcga gagttgatcg tcggagaatt ggccggtttg
     1141 cattagctgg gcctgctcga gtgtgttgct tccgcctgca ggtggttgta cgaaaggact
     1201 cgactgattc gggttaagcc acggagggcc gcccggaacg gtcccctgcg gttgcggtgc
     1261 tagttcctgc ggtatttgac cttgtcgttg ttggccggcc acttcgcagc tcttcgtatc
     1321 gccttgcgaa catttccgcg cgttttcata gcggtcttgg gcctgctgca acgcttgcgc
     1381 tctttgctgt tcttcttcct gagcgaggaa ttttacgcgc tcttgaacga gcaggtcttg
     1441 ctctcttcca gcctgggagt ccggattcac ggcaggcatc aagtagccgt agcgctgaag
     1501 tagctgggtg accacggaac ggaatcgcac atcattctgg agacgttcga aaatcgcgtc
     1561 ttgggctagg tcgcgctccg tgataatttg gccctggtcc gttgcatcct tagccaccca
     1621 gcgcttcact tcgaccataa gccccgggct cttaactaaa actgccttga tgtcctctgc
     1681 cgatgctcct acttgggcga gattatggcg agcgagttcc ggaccgctga cagggttctg
     1741 ccgcgcgacc tgagcccctg tgatcgttga caataaaaag aacgcccaca gggagcaggc
     1801 gaaccggatc gcgcacccgc gagttcctct ctgaagcatg ctgagccgca gattactggt
     1861 cgccatcgca gggatggttc ccatagcgcg ccctccatgg ttgatcggac gacccattat
     1921 gaagcgaagt actcggctaa gcaaaggttc aatgtgttcc atcctacgtc gttcctcaac
     1981 tgctgcatac gcggactcga atttgcttac aatcctgcgc acttcgactc tcagatgcac
     2041 gtagccattc aaggtttgcc gcgggatctt ggccagcctt tgggatatta ccggcattgc
     2101 cgcgcggctc gtttgaccgc acacggaact cgccgctata attgcctttt aaatagcttc
     2161 cgcagggaaa ctccagagaa gaaatccagg agcagtcatg ataaaggcaa cactgttgcg
     2221 cccgggaatg atcatcaagc acgagggcga tctccacgta atttatagtg tcgatcaccg
     2281 gacgccggga aataagcgag ccgcgatggc caccaagatg cgtaatttgc gcaacggctc
     2341 gatgctggat caccgcttcc gcgctgagga atttctcgac aaagtcacgg tggacgaaat
     2401 cgaattcgac tacctctata gcgacggtga gggccaccat ttcatgaaca cccagaatta
     2461 cgaacagctc gcgttgcaga atgatgtggt cggcgacagc aaggactacc tcatccctaa
     2521 catgcccgtg aagatcgagt attacgacga gaagcctatc ggagttctca tccccgatac
     2581 cgtcgatctc atggtggtgg aaacagagcc gagcattcag aaggccacag ccagctccgt
     2641 gatgaagccc gcgaagcttg agaccggcct agtgatcaac gtgccgccgt ttgtaggcac
     2701 tggcgacaaa gtaaaggtcg acaccaccga aggccgctac atccagcgcg ttcaataatt
     2761 caactttgca gattcggtaa gagctagcgc gcggcggaag attttgcgcc gaagacacgc
     2821 gtaaaaatag cgtccacggc tcgcaactgc cgttgcaaat cgaaagtgtg cgccagcgct
     2881 ttttcatcca gatgtttccg gatgttctgg tcggcgacaa tgcgctcgcg gaaactggtt
     2941 tccgattccc aagcggccat ggcgtgcgac tgcacccatt tgtacgcgtc ctcgcgaggc
     3001 gcgccgtgct cgactaaatc ctgcagcaat tgccctgaga aaaccaggcc cttggtcgcg
     3061 tcgagattgc ggcgcattct ttctggaaac actttcatgt tggccacgat ttcgctggtt
     3121 cgatgcagca tgtaatccac caaagtcgtg gagtccggca agatcactcg ctcgacggaa
     3181 ctatgcgaaa tgtcacgctc gtgccagagg gcgacgtttt ccatcgcagc cagtgaattc
     3241 gaccgaacca cgcgcgccag gccgcaaatt tgctcgctgg atacgggatt gcgtttgtga
     3301 ggcatcgcgg agctgccgcg ctgctcgccg ccaaacggct cttcaacctc gcgaacttca
     3361 gtgcgctgga gatggcgaat ttcaagcgcc acgcgctcga gcgatgccgc gacgattgcg
     3421 agtgcagaga gatattgcgc gtgccggtcg cgctcgatta cctgcgagct aatcggcgcc
     3481 gtgctcagtc caagccgctg gcatattttc tgctcgatct ccgcgccaag gtgggtgcac
     3541 gttccgacgg cgccggaaat cttgcccacc gccatctcgc gcgccgccgt ctcaaagcgt
     3601 gtcgcatcgc gccgattttc cgcgtaccag ttcgcaattt tcagcccaaa agtaatcggc
     3661 tcggcgtgga tgccgtgcgt gcggccgatc tcaggcgtgt ccttgaattc ccaagcacgc
     3721 ttttcgagga tttcgccgaa tcgcgcgata cctttttcga tcaagttcga cgcatcgcga
     3781 acgagcagcg cttgcgccgt gtccaccaca tcgttggaag tgagtccgta atgcagccag
     3841 cgcgcagctt ccggatcgcc aaccgtttcc tgcacggcga tggtgaacgc gattacatcg
     3901 tgccgcacct tcgcttcaat ctcattgatc cgcgccacgt tgatgcgcgc gcgggcgcgc
     3961 agtttcgcag cggcttcgcg aggcacaatt ccggcctcgg cgagcgtttc cgaagcggcg
     4021 agttccacat cgagccagcg ctgaaactta ttttcgtcgc tccagacgcg gcccatctcc
     4081 gaacgcgtgt aacgcgcgat caaatccggc tccttgaatt caggttgcta gcaggcgaat
     4141 ttcctattct atgatacccg aaacagggat gcagccgtgc tcgcaaaggt agaggtagaa
     4201 gttcgctgcc acctcgcatg cggaacggca gtggcgagat gttgtgctgg ggcgacgact
     4261 ctaggccgag cagcgatctt cgggtagctg tactccccag aagttttgag cggggtgaag
     4321 aatttcgatg cccaattcga aagaacctgg aaccttcgga cctttcgccg agatcacgcg
     4381 aaactcgcgc acctcttgag agcgcgcgtg aagaagctga atccgtgagc ccacgggagg
     4441 caggaacgga
//
LOCUS       DATNDT010000410         4094 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1807526, whole genome shotgun sequence.
ACCESSION   DATNDT010000410 DATNDT010000000
VERSION     DATNDT010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1807526"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1111
                     /locus_tag="VN788_13330"
     CDS             <1..1111
                     /locus_tag="VN788_13330"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HXU21559.1"
                     /translation="NTHVVYHHGLLFALKEDSPPVAMNPNTLETVDDYYTFGGGLHSL
                     THTAHPKFDKATGEMISYGYEAKGLDTNDVYVFSANAKGHITWETWLKVPYVGMIHDF
                     AVSQKYIAFLVIPMATNVARMKEGQVHFAWDSTLPTWFGVLERGGDGKDVRWFKGPER
                     CATHVMGAFSDGNKIYVDMDMALKNQFPFFPNLHGEPFDPKAAAGTVHRLSVDLSDKT
                     PRDYNMEELYPQTGALPRQDDRYQTIPYRYGFMPTSDPTKPINPKLRRMAFRPTNSYT
                     MFDHATRKTSEFWIGDDSSLQECCFVPRNKDAAEGDGYLIGVANRMLEAGRSDLIVVD
                     TQHMDDKPIAMVRLPFRTYSQVHGWWVPGDDLAQT"
     gene            1127..2695
                     /locus_tag="VN788_13335"
     CDS             1127..2695
                     /locus_tag="VN788_13335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXU21560.1"
                     /translation="MTTTAPSPALSRDRQSLFSSGMALIWAIALADLLFHIYFNNRYG
                     YFRDEFDYMACGNHLAWGYVDQPPLVPFLVRICRAVLGDSLRSIRFIPALASSLLIVQ
                     TAVLARELGGKRFALLLSAVTVLIAPQYLSNGSLLTTNCLEPNLWMGCAYFAILAIKR
                     SEPRYWLWFGVIAGIGLEEKYTIALFGFGIVAGLLLTEHRCVFRDKWIWLGGGAAFLI
                     FLPNLLWNLHYHWPFLQLMHNIRAEHRDVVLGPVEYFLQQTILIHPFTAPIWIAGLAA
                     LFFWARLKPYRFLGWTYVICYAVLFALHGENYYLAPVYPMLFAAGAIVIQSALERPIG
                     AWLKPAIVIILLAAGAHLVPVTVPVFSPDHFIAYMKGLPFKLPVMEYQHRRAVLPQWY
                     ADQFGWNEIVAETAQAWNQLPPGRADCAIFAQDYGQAGSIDFLGRSYGLPAALSGNQT
                     YFLWGPRGYSGNCMIVLDDRPDRLKQLFENVQYVGTSADNPYALEKRIGVFICRGSKF
                     GTLAQIWPKLKHWR"
     gene            2806..3783
                     /locus_tag="VN788_13340"
     CDS             2806..3783
                     /locus_tag="VN788_13340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21561.1"
                     /translation="MRRGLILAATSAFLFGILASKPSMAAAARPTNDCDRACLNGFVD
                     QYMAAVAAHDPSKLPTTASVRYTENNVEMKLGEGLWQTSDGWGSYKVYVDDPQTGQVG
                     FLGVANEDTHLSCFGARLKVVNKKVSEIEVIVARPDRPGPPGSGNLTGGPQNLKDKPL
                     FSEDEPVSERVSRQKLIALADGYFSTIQQNTGKIYTSFDPDCQRMENGTITANNPNGS
                     GVAKMGCEAQLKTGLLVFVTRCRDRRYFVDEQKQMVFSATFFDHAGNVRDFKLADGTE
                     HKMGPPFDRPYTFLIFELFKIKNGKIRQIEAVLDTVPYYMPSPWVKGSK"
     gene            3797..>4094
                     /locus_tag="VN788_13345"
     CDS             3797..>4094
                     /locus_tag="VN788_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21562.1"
                     /translation="MRISGKVFGFAVVLALTTLAPALRAQAIAPPPDYDPDFNYDCDR
                     ACLNGFVDQLIAAMVAHDSSTLPLARDVKYTENGQILKIGDGFWGTASSLPTYKI"
BASE COUNT          848 a         1191 c         1113 g          942 t
ORIGIN      
        1 gaatactcac gtcgtttatc accacggctt gcttttcgcg ctgaaggaag atagccctcc
       61 ggtggccatg aatccgaaca cgctcgaaac ggtggacgac tactacactt tcggaggcgg
      121 ccttcacagc ctcacccaca ctgcgcatcc caagttcgat aaagccacgg gcgagatgat
      181 ctcgtacggc tacgaagcca agggactcga tacgaatgac gtttacgttt tctcggcgaa
      241 tgccaagggg cacatcacgt gggaaacgtg gctcaaagtt ccctacgtgg ggatgatcca
      301 cgatttcgcc gtttcacaga agtacatcgc gttcctggtg atcccgatgg ccacgaacgt
      361 cgcgcgcatg aaagagggcc aggtgcactt tgcatgggat tccactctgc ccacttggtt
      421 cggcgtcctt gaacgaggcg gcgacggcaa ggacgttcgt tggttcaaag gaccggaacg
      481 ctgcgccacg cacgtgatgg gcgccttctc cgatggcaat aaaatttacg tcgatatgga
      541 catggctctc aaaaatcagt ttcctttttt cccgaatctt cacggggagc cgttcgatcc
      601 aaaggcagcc gccgggactg tgcatcgctt gtcggtcgat ttgtcggata agacgccgcg
      661 cgattacaac atggaggaac tctatccgca gactggcgct ctgccgcgcc aggacgatcg
      721 ctaccagacg attccctatc gctacggctt catgccgacc agcgatccca ctaagccgat
      781 caatccgaag ctacgccgca tggctttccg tccgacaaat tcctacacga tgttcgatca
      841 cgccacgcgg aaaacttccg aattttggat tggcgatgat tccagccttc aggaatgctg
      901 ttttgtgccg cgtaataaag acgcagccga gggcgacggc tatctgatcg gcgtcgcgaa
      961 tcgtatgctg gaagcgggcc gctcggatct tatcgtcgtg gatacgcagc acatggacga
     1021 caagccgatc gccatggtga ggctgccgtt ccgcacctac agccaggtgc atggctggtg
     1081 ggttcccggc gacgatctgg cgcagacctg acgcgcgcat cgaacattga cgacaaccgc
     1141 tccttcaccc gcgctgagcc gcgaccggca atcccttttt tccagcggca tggctctgat
     1201 ttgggccatt gcccttgccg acctgctctt ccacatctat ttcaataatc gctacggata
     1261 ttttcgcgat gaatttgatt acatggcctg cgggaatcat cttgcgtggg gctatgtcga
     1321 tcagccgccg ctcgttccgt ttctggtgcg tatttgtcgc gccgtgctcg gcgattcgct
     1381 ccgcagcatt cgctttattc cggcgctggc ttcttcgctg ctgattgtgc agacagcggt
     1441 gctcgcccgc gaactgggcg gaaagcgttt cgcgctgctg ctgagcgcgg tgactgtgct
     1501 gatcgcgccg cagtaccttt cgaacggtag cctgcttaca acgaattgtc tcgaaccaaa
     1561 cctgtggatg ggctgcgcct atttcgcaat tctcgccatt aaacgcagtg agccgcgtta
     1621 ctggctttgg ttcggcgtga tcgcgggcat tggcctggag gaaaaatata ccattgcgct
     1681 tttcggattc ggaattgtcg ccggactgct acttacagaa caccgctgcg tcttccgcga
     1741 caagtggatc tggctcggcg gcggcgcagc tttcctcatc tttctgccga atctgctttg
     1801 gaacttgcat taccactggc catttcttca gctcatgcac aatattcgag ctgagcatcg
     1861 cgatgtagtc ctcggtccag tcgaatactt ccttcagcag acaattctca ttcatccatt
     1921 caccgcgccg atatggatcg cgggcctcgc cgcgctgttc ttctgggcgc gcctgaaacc
     1981 gtaccgcttc ctcggctgga cgtatgtgat ctgttatgcg gtcctgttcg ccctgcacgg
     2041 cgagaattat tatctggcgc ccgtctatcc gatgcttttc gcggcgggcg ccattgtcat
     2101 ccaatcggct cttgagcggc ccataggcgc gtggctgaaa ccggcgatcg tcatcatact
     2161 gctcgctgct ggtgcgcatc tcgtgcctgt caccgttcct gttttttcgc ccgatcactt
     2221 tatcgcctat atgaaaggcc tcccgttcaa acttcccgtc atggaatacc agcaccggcg
     2281 cgccgtgctg ccgcagtggt acgccgatca gtttggctgg aacgaaatcg tcgccgaaac
     2341 cgcgcaagca tggaatcagt tgccgccggg acgcgccgat tgcgccatct tcgcccagga
     2401 ttacggccag gccggctcga ttgattttct cggacgcagt tacggcttgc ccgcggccct
     2461 aagcggcaat caaacctact ttttgtgggg accgcgcgga tattccggca attgcatgat
     2521 tgttctggac gatcgcccag accgcctcaa gcagcttttt gagaacgttc aatacgtcgg
     2581 cacctcggcg gataatccct acgcgctgga aaagcggatc ggcgttttca tttgccgcgg
     2641 atcgaaattc ggtacgctcg ctcaaatctg gcccaaattg aagcactggc gctagaccga
     2701 ccgaattgca gcaaactgtg cagtcccaag agagggttgc gacttgatat cccttctgat
     2761 atagatgcgg cgcattcaaa ttgaagtggt ccacggggga aagtaatgcg cagaggcttg
     2821 attttggccg cgacgtccgc ctttcttttc ggcattcttg ccagtaagcc gagcatggct
     2881 gccgcggcgc ggccaacgaa cgattgcgat cgcgcttgcc tgaatggatt cgtcgatcaa
     2941 tacatggcgg cggttgccgc gcacgatcca tcgaagctgc caacaactgc gagtgttcga
     3001 tacaccgaaa ataacgtcga gatgaagctc ggtgaaggat tgtggcaaac atccgatggc
     3061 tggggatcgt acaaggttta cgtcgacgat ccgcaaacgg gccaggtcgg atttctgggc
     3121 gtcgcaaacg aagatactca cctatcgtgt ttcggggcac gccttaaagt cgtcaacaaa
     3181 aaagtttccg aaattgaagt catcgtcgcg cgccctgacc gcccgggtcc gcccggctct
     3241 ggtaatttga cgggaggtcc gcaaaacctg aaagataagc cgctcttttc cgaagacgaa
     3301 cccgttagcg agcgcgtctc tcgccaaaag ctgatcgctc ttgcggatgg ctacttttcc
     3361 acgattcagc agaacaccgg caagatctat accagcttcg atcccgattg ccagcgcatg
     3421 gaaaacggaa ccatcacggc caacaatccg aatgggagcg gcgttgccaa gatggggtgc
     3481 gaggcgcagc taaagacggg gcttcttgta ttcgtcacgc gctgccgcga ccgccgctac
     3541 ttcgtcgacg aacaaaaaca gatggtgttc tccgcgacat ttttcgatca tgccggaaac
     3601 gtgagggact tcaagctggc ggatggcacg gagcacaaaa tgggtccgcc attcgatcgc
     3661 ccatacacat tcctcatctt cgagcttttc aaaattaaga acgggaaaat ccgccagatc
     3721 gaagctgtac tcgacaccgt gccctattac atgcccagtc cctgggtaaa gggttcgaag
     3781 tagattgcga ggctccatgc gaatcagtgg aaaagtgttt ggttttgcag tggttctggc
     3841 gctcacaacg ttggcgcccg cgttgcgcgc gcaagcgatt gcgccgcccc ccgattacga
     3901 tcccgatttc aattacgatt gcgaccgcgc ttgtctgaat ggctttgtcg atcagctcat
     3961 cgccgcgatg gttgcgcacg attcttcgac tttgccgctc gcgcgcgatg tgaagtacac
     4021 cgaaaacggc caaatcctca aaatcggcga cggcttctgg ggcacggcca gttccctgcc
     4081 cacttacaaa atct
//
LOCUS       DATNDT010000411        11673 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2975047, whole genome shotgun sequence.
ACCESSION   DATNDT010000411 DATNDT010000000
VERSION     DATNDT010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11673
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2975047"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..670)
                     /locus_tag="VN788_13350"
     CDS             complement(194..670)
                     /locus_tag="VN788_13350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HXU21563.1"
                     /translation="MRVSVLTISDSVAAGKAEDRSGPSLMARCEELGWEIASSAVCAD
                     DRPAIEAFLRGAADSNSADLILTTGGTGVGPRDVTPEATAAVCERLIPGFAEQMRNAG
                     REKTPRAILSRAAAGIRGQAIIINLPGSPRGAVESLDTIADLLPHAVAVLHGAHHG"
     gene            complement(683..1444)
                     /locus_tag="VN788_13355"
     CDS             complement(683..1444)
                     /locus_tag="VN788_13355"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU21564.1"
                     /translation="MTAMTDVRVLVVDDNALILDLLMKGLATHCETQAAADGADALLK
                     IVDNPPDLILCDYRMPGLDGRQLYEKLRGRTQTKLIPFIFLASRSDVEEKLRPIVEGA
                     EEFVVKPFFINDLVRRTKKVIDRLRLEKLQNRAVRPGVIQGRLEEMSILDLLQSLEMG
                     QKSCRLTIRHETDRCELFFATGQCADGRLGPLEGEDAVFQVMRWPAGEFEIDFNSAPT
                     RTTISRTTTGLLMEGLRLMDEGQRDSATEGVETTG"
     gene            complement(1441..1950)
                     /gene="moaC"
                     /locus_tag="VN788_13360"
     CDS             complement(1441..1950)
                     /gene="moaC"
                     /locus_tag="VN788_13360"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HXU21565.1"
                     /translation="MPTNKKLSHYDQTGRVRMVDVTAKPATSRTAIAHAFVRMQSATL
                     ASVRRLKSPKGNPLEIARIAGITAAKRTSELIPLCHPLSLTHVDVSAKLCQNGIEITS
                     EATATGPTGVEMEALVAASVAALTIYDMCKALERGMDISEIYVVKKSGGRSGTYERSK
                     TRSRKSAKR"
     gene            2083..2421
                     /locus_tag="VN788_13365"
     CDS             2083..2421
                     /locus_tag="VN788_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015162729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HXU21566.1"
                     /translation="MRTELLTNSHFANVIEPVHIISRKKLLEAAQEHSGLGESLDVWY
                     RIAKKANWKNLAEVRRVFPSADAVEGFIVFNIKGNAYRLIVEINYHTGRVFLRHVLTH
                     AAYSKGDWRR"
     gene            2418..2819
                     /locus_tag="VN788_13370"
     CDS             2418..2819
                     /locus_tag="VN788_13370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXU21567.1"
                     /translation="MSTLTISREYGALLAKIPPKVIRTEKENEAYRGILSDLDRRHKM
                     LTPAEKELSDLLTLLIEDFEEKRYRLPRAKPQDAVRFLMEQHELRQKDLVNIFGTPSI
                     VSEVLSGRRELTTTHIKRLSERFHVSPEIFF"
     gene            complement(2906..3592)
                     /locus_tag="VN788_13375"
     CDS             complement(2906..3592)
                     /locus_tag="VN788_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HXU21568.1"
                     /translation="MDRAAKRAFKTNLFEQFARIGKALANGNRLEMLELLAQKERSVE
                     ELGRLTEMSTANTSQHLQVLRRARLVDATKDGLYVRYRLADPRVFELWQALRDLGKDR
                     IAEIDRVVHSFLKERSTLKPVTTKELRQRLKDGSAIILDVRPAEEYGAGHIRGARSIP
                     LGELKSRLRELPKSREIVAYCRGPYCVLADEAVVLLRSHGLKAFRFERGFPEWKAHGF
                     PVEMAFPVAG"
     gene            3686..4450
                     /locus_tag="VN788_13380"
     CDS             3686..4450
                     /locus_tag="VN788_13380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21569.1"
                     /translation="MSAKIYIPSTLFTLFLVAGFSTTACRAQTIPLGTTLPVRLEQTL
                     NSDKGQPGKVVTVKLTQAVPLDHARRIRKGAVVIGHIVAAGPREEAKTRAQLILRFDT
                     LRFSHQDVPVVVTLRAMAGYVQVEESALPTSNEESIPWTTVQIGGDIVYRGGGEVTTS
                     SGEIVGKPVPDGVLASLLAPRTEISSANLHCGPDSSPHALGPFGSRACGLYGLPHLRI
                     ARDGFAEPRGEITIISSVQPLKIASGAELLLQVVAP"
     gene            complement(4489..5001)
                     /locus_tag="VN788_13385"
     CDS             complement(4489..5001)
                     /locus_tag="VN788_13385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU21570.1"
                     /translation="MKIQPFVLVALAISFVSVATIAQEPVVGVTGAEADALFTSKNPR
                     LNTNKQAAYHIMKDLLEANHWDEANKWLTEAYHQHNPNVVSGLAPVVKYFESARKPSP
                     VPEHLTKTRIVAVVAEGDLVIVVTPRELTDPRDSSKKYTTSWFDMWRFKDGKADEHWD
                     GATIAPLRTK"
     gene            complement(5068..6345)
                     /locus_tag="VN788_13390"
     CDS             complement(5068..6345)
                     /locus_tag="VN788_13390"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HXU21571.1"
                     /translation="MTILGSYPDLLIKPHHIARMVLEGLRVHIPQSGHGGNFGGGTST
                     SKITIGEAIANGAVFEIARANNRPPLRFEIHQLDLKSVGAKQAFSYRVDMHNPEPPGE
                     IQSAGSFGPFNSQEPSKTQLSGTYSFERANLGAFQGVAGTISSSGTFSGDLAHINAQG
                     YTDAPNFEVARSGHAARLSTQFQIGVDALNGDIVLSRVNAFYGKTKIAVNGSVAKKPD
                     YRSKFTALDFAVSEGRIQDVLQLFVHALKPPMAGVTSFQAHVTVPPEGKPFLKEVTLD
                     GDFGIGGGHFETPQTQAKADDLSATARGDKKTAQEQKQNPSTPQENVISDLRGHVSLR
                     DGVARFSNLSFKVPGADARLNGTYNLLTEAVDFHGTLKMDTKFSKSASGIKSIFAKII
                     GPFIAKKHGSVVPVVMNGTYDHPHFGLDLNPAK"
     gene            6774..7361
                     /locus_tag="VN788_13395"
     CDS             6774..7361
                     /locus_tag="VN788_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU21572.1"
                     /translation="MGSSDRSPSLWVAKYRVNVTCILQAIFGIVPAVVGKSQSKHRSA
                     CPVSISLEMLGDRWSLLIIRDLMVRGYRTFKEFRGSGEGIATNILSDRLRKLQAVGII
                     TAGAEKTDARKVNYRLTEKGIGLAPVLLELLIWGARHEETGAPCAVIDEMEKNRQQVL
                     AETRRRWQEQDPTPILPKFADREGHRNLTKARRGA"
     gene            7447..7878
                     /locus_tag="VN788_13400"
     CDS             7447..7878
                     /locus_tag="VN788_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007880486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU21573.1"
                     /translation="MQLNAYLNFNGQCEAAFKFYEKCLGGKIEAMARFEDMPEGGGVS
                     PEWRSKIMHTQLNAAGTVLMGSDAPDGRYQKPQGFAVSLQTSDPAEADRVFNALSENG
                     AVSLPIQETPWAVRFGMLVDRYGIPWMVNCQKAAAAAPAQS"
     gene            7930..9108
                     /locus_tag="VN788_13405"
     CDS             7930..9108
                     /locus_tag="VN788_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HXU21574.1"
                     /translation="MSKVRVLVGTRKGAFILESDGKREKWEVKGPHFAGWEMFHLKGS
                     PVDPNRIYASQTSGWFGQVIQRSSDGGKTWETPGGEKMPTPGGPPPGKSNHFLYDASP
                     ETGKPLTTHQWYDGTQHPWEFKRVWHLEPSPTDPDTVYAGVEDAAIFKSTDGGKSWNE
                     LSGLRGHGTGPKWQPGAGGMCLHTIVLDPSNPGRMYIAISAAGAFRTDDGGKTWKPIN
                     RGLKSQYIPDPTAEVGHCVHRIAMHKSRPDVLYMQKHWDVMRSNNAGDQWEEVSGNLP
                     TDFGFVIDVHAHEPETIYVVPIKSDSEHFPLDGKLRVYRSKTGGNEWEVLSKGLPDRD
                     CYVNVLRDAMAVDSLDSCGIYFGTTGGQVYGSADAGNSWAPIVRDLPAVLSVEVQTLP
                     "
     gene            9105..9401
                     /locus_tag="VN788_13410"
     CDS             9105..9401
                     /locus_tag="VN788_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HXU21575.1"
                     /translation="MILVVLPPHLRTLARVTGDVELEVAGQPTVRSVLDALEARYPML
                     CGTIRDQATKERRPFLRFFACEGDLSHESPDAPLPEAVASGKEPFFIIGAIAGG"
     gene            complement(9658..10509)
                     /locus_tag="VN788_13415"
     CDS             complement(9658..10509)
                     /locus_tag="VN788_13415"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HXU21576.1"
                     /translation="MNKNIELLGSYWTLAVGAEPHTNHEFSTVDFKERVETIARVGFK
                     GVGIWHADLEHTLKKRSLKEMKKILDDNGIRHLELEFLGGWFLDGEEKKASDIERKKL
                     LEAADVLRPHHIKVGHFFKTDATMPRMIESFAALCADGANHGTPIGFELMPFCDIDSV
                     EKALQLVEGAGAKNGGICLDLWHLAKLKIPYERVASIPLKYVTSIEINDGTHECSWSL
                     HEDTINHRLLCGEGEFNPKDFVSKMLKAGYDGPWGIEVLNAELRKKPLEEMANSAFRT
                     TLAQFPS"
     gene            10663..11445
                     /locus_tag="VN788_13420"
     CDS             10663..11445
                     /locus_tag="VN788_13420"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU21577.1"
                     /translation="MQLAGQTAIVTGGGRGIGRAIARRFAAEGAAVVVTARTESEVRE
                     VVSEIQSAGGKAAAITADISHEPDCEKIVRGAREAFGAIHILVNNAGIFGPVRPVEKI
                     SAGEWDTVLAVNLRAPFLLARLILPEMYERGSGAIVNISTVGAKAAYPMNCTYAASKA
                     GLDGLTRTLAAESARKGVRVNAICPGPVTETKMSKDLTREFAKQLGSNGDEVLNRMIA
                     GTLQGRPQTADEIASAALFLVSGQASAITGQTLNVDGGLVFY"
     gene            complement(11399..>11673)
                     /locus_tag="VN788_13425"
     CDS             complement(11399..>11673)
                     /locus_tag="VN788_13425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21578.1"
                     /translation="IVILQAYFVRELIAAELLFGIVFAAIFLFVAICYTLGSAWEYGV
                     VWGEVGVRAIATTARRGYGALEYVSNGVMESISKRQGRHQHSGSAQ"
BASE COUNT         2480 a         3484 c         3207 g         2502 t
ORIGIN      
        1 atttgctcgc gcctgctttg cgcttcggac tcattgccac cctcctcaat ttaaaagcgg
       61 attgcctgtc gcggagatga acgcccaaac gccgggagtg tagcgcacaa tgaacgtcat
      121 ttgcacgctt gtggcgcagg ctctcctgcc tgcgctgctg tgatgcggtg agccatgctt
      181 tcagaattga ggcctatccg tggtgcgcgc cgtgaagcac ggccacagcg tgtggcagca
      241 ggtcggcaat cgtatcgaga gattccacgg cgccgcgcgg gctgcccggc agattgatga
      301 tgatcgcctg accccgaatg ccggcggcgg cgcgcgaaag aattgcccgc ggcgtttttt
      361 cgcgtcccgc gtttcgcatc tgctcggcaa atcccggaat caacctctcg caaacagctg
      421 ccgtcgcttc cggcgtcaca tcgcgagggc ccactcctgt gccgcccgtc gtcaaaataa
      481 gatccgcact gttggaatcg gccgcgcccc ttaaaaaagc ttcaatcgcc ggccggtcgt
      541 ctgcgcaaac tgcagaagac gcaatctccc aacccagctc ctcgcatcgg gccatcaacg
      601 aaggcccgga gcggtcctcc gctttgcccg ccgccacgga atcgctgatt gtaagaacgg
      661 aaacgcgcat gaggacccgg tgctacccgg tggtttccac gccttcggtg gcgctgtcgc
      721 gctggccttc atccatcagg cgcagccctt ccatcaacag gcccgtggtg gtgcgggaaa
      781 tcgtcgtgcg cgtaggagcg gaattgaaat cgatctcgaa ctcgcccgcc ggccagcgca
      841 tcacttgaaa aacagcatct tcaccttcga gcggccccag ccgcccgtct gcgcattgcc
      901 ccgtggcaaa aaataattcg cacctatccg tttcatggcg aatcgtcaag cgacacgatt
      961 tctgtcccat ttcgagcgac tgcagtagat cgaggatgct catttcctcc agccgtcctt
     1021 gaatcacgcc cggccgcact gccctgtttt gcagtttttc gaggcgcagt cggtcgatca
     1081 cttttttcgt gcggcgcacc agatcattaa tgaagaatgg cttgacgaca aactcctccg
     1141 cgccttccac gatcggcctc aatttttctt ccacgtcgct gcggctcgcc aggaaaataa
     1201 acggaatcag cttggtttgt gtgcgcccgc gcaatttttc gtagagctgg cgcccgtcga
     1261 ggccgggcat gcggtagtcg cacagaatca ggtccggcgg attgtccacg attttcagca
     1321 aagcgtccgc accgtcggcg gccgcctggg tttcacagtg cgtcgcgagc cccttcatca
     1381 aaaggtcgag gatcagcgca ttgtcgtcaa ccacaagcac acggacatcc gtcatcgccg
     1441 tcatcgtttc gcgcttttcc tggaacgcgt cttggagcgc tcgtacgtgc cgctgcgccc
     1501 ccccgacttt tttaccacgt aaatttccga gatatccatg ccgcgttcga gggccttgca
     1561 catatcatag atcgtcagcg ccgccacgga cgccgcgaca agcgcttcca tttccacccc
     1621 agtcggtccc gtagccgtcg cttccgaagt tatctcaatt ccattctggc atagcttcgc
     1681 gcttacatcc acgtgcgtca atgagagggg gtgacaaagc ggaatcagct ccgaggttcg
     1741 cttcgcggcc gtgattcctg caattcgcgc gatttccagc ggattgccct tgggcgattt
     1801 cagccgccgc acgctggcaa gcgtcgcgga ctgcatccga acaaacgcat gcgcgatggc
     1861 ggtgcgcgac gtcgctggct tggcggtaac gtccaccatg cgaacccgcc ccgtctgatc
     1921 gtagtgtgaa agtttcttgt tggtcggcaa tttgtcggcg ctcggctcag tgcacaaggt
     1981 cgcacaagca tagcgcccgc gcaagtgcca ctgcccaggg accttcgagc gaagaacccg
     2041 tgagggggcg cctcatcgtc gcgctctgcc ccagttcgcg ttatgcgaac tgagctattg
     2101 acgaattcgc attttgcgaa tgtaatagag ccagtgcata ttatcagccg caagaagctg
     2161 ctcgaagcgg cacaagagca ttccggtctc ggcgaaagcc tcgacgtctg gtaccgcatc
     2221 gccaagaagg cgaactggaa aaacctggcg gaagtgaggc gcgtatttcc cagcgcggat
     2281 gcggtcgaag gtttcatagt gtttaacatc aaggggaatg cgtatcgttt gattgttgag
     2341 attaactatc acacgggaag agtttttctt cggcatgtcc tgacgcacgc cgcatatagc
     2401 aagggagact ggaggagatg agcaccctaa caattagccg cgagtacggc gcactgctgg
     2461 ccaagattcc tcccaaggtg atccgtacgg agaaggaaaa cgaagcctat aggggaattc
     2521 tctctgatct ggaccggcgg cacaaaatgt tgacacccgc tgaaaaagag ctttccgact
     2581 tgctgaccct attgatcgag gattttgaag agaaacgcta tcgcctgccg cgagccaagc
     2641 cccaggatgc ggtccgtttt ctgatggaac agcacgaact ccgccagaag gatttagtga
     2701 atattttcgg aacgcccagc atcgtgtccg aagtattaag cggccggcgc gagctcacca
     2761 caacccacat caaacgacta agcgagcgtt tccacgtctc gccggagatt tttttctagc
     2821 ctctgccaag gcaacttggc agctatttgg ttctcagagg cgcattgcgc cccatccgtc
     2881 gtttgatgtt gtcgaagaca ttcacttatc ccgcaaccgg gaatgccatt tcgacaggga
     2941 atccatgggc cttccactcc gggaagcccc gctcaaagcg aaacgccttc aggccgtggg
     3001 aacgaagcag caccaccgcc tcgtcagcca gaacgcaata cggccctcgg cagtaagcca
     3061 caatctcccg gctcttgggc agttctcgaa ggcggctttt tagctccccc aagggaatcg
     3121 accgcgcccc ccgaatatgg ccggctccat attcttcggc gggccgcaca tcgaggatga
     3181 ttgcgctgcc gtccttcagg cgctgcctca gctctttagt ggtcaccggt ttcagggtgc
     3241 tgcgttcctt cagaaaagaa tgcaccacgc gatcgatctc cgcgatgcga tccttgccaa
     3301 ggtcgcgcag cgcttgccac agctcaaaga ctcttgggtc cgcaagacga taccttacgt
     3361 acagtccatc ctttgtggca tccacgagac gcgcacggcg aagcacctgg agatgctgcg
     3421 aagtattcgc agtcgacatc tccgtcaggc gccccagttc ctcgacgctg cgctccttct
     3481 gagccagcag ttccagcatt tctaggcggt ttccgtttgc aagcgccttt ccgatacggg
     3541 cgaactgctc gaagagattg gtcttgaacg ctcgttttgc tgctcgatcc atgctagtat
     3601 tcaagcattc gcttgaacac ttgtcaagat aaggtaaccg gcgcccgatc atgatttccc
     3661 tcttaccgcg actcggggtg cagcgatgag cgctaagatc tacataccgt caacgctttt
     3721 cacactgttc ttagttgcag gcttttcgac caccgcttgc cgggcgcaaa cgatccctct
     3781 cggaaccacg ctaccagtgc gcttggagca gaccttaaac agcgacaagg gccaaccggg
     3841 gaaggttgta acggtcaagc tcacccaagc ggtgccgttg gaccatgcgc ggcggattcg
     3901 taaaggagcg gtcgtcatcg gacatatcgt cgctgccggc cctcgtgagg aggccaaaac
     3961 ccgggcccaa ctgatattga ggttcgacac gctaaggttc tcccaccagg acgttcctgt
     4021 tgtggtaacc cttcgagcca tggctggcta cgttcaagtg gaagagagcg cacttccgac
     4081 cagcaatgaa gagagcatcc cttggaccac cgtgcaaatt ggaggggata ttgtctatcg
     4141 cggtggtggc gaggtgacaa cgagcagcgg ggaaatcgtc ggcaagccgg tcccggatgg
     4201 ggttctggcg agcctgctag ctccgcgaac ggaaatcagc tccgccaact tgcattgcgg
     4261 gccggactcg tctccccacg ccctcggacc ttttggttcc cgtgcatgcg gtctgtatgg
     4321 gttgccccat ttgagaattg cgcgtgacgg atttgccgag ccgcgaggag aaattacaat
     4381 tatctcgtcc gtacaaccgc tcaaaattgc gtcgggagcg gaactcctgc tgcaagtggt
     4441 cgcgccttag cgaagcgacg gccaaacgtc gaacccaagg atcggttgct atttggttct
     4501 cagaggcgcg atcgttgcgc cgtcccagtg ctcgtcagcc ttgccgtcct tgaaccgcca
     4561 catgtcaaac cacgacgtgg tgtatttctt cgacgagtct cgcgggtcgg tcagttctcg
     4621 cggcgtcacc acaatcacca agtcgccttc cgctaccaca gccacaatcc tcgttttcgt
     4681 aaggtgttct ggaacgggag atggcttgcg cgcagactcg aaatatttca ccaccggggc
     4741 aagtcccgag acgacattcg gattgtgctg atggtacgct tccgtcagcc acttgttggc
     4801 ctcgtcccag tgattcgctt cgagcaggtc cttcatgatg tgataggcgg cctgcttgtt
     4861 ggtgttcaac cgcgggttct tgctggtgaa aagagcgtca gcctctgcgc ctgtcacgcc
     4921 aaccacaggc tcttgggcaa tcgtggccac agagacaaac gagatcgcga gcgccactag
     4981 gacaaacggt tgtattttca tgccgtgcta aatacatccg aatgcgggac ggtgtcaagg
     5041 ccgtatcgat ctctgcggaa atagccatca tttagcggga ttcaggtcga ggccgaaatg
     5101 tggatggtcg taggtcccgt tcattaccac ggggaccacc gagccgtgct ttttagcaat
     5161 gaagggaccg atgattttcg cgaatatcga tttgattccg ctggcgcttt tagagaattt
     5221 cgtgtccatc ttcaaagtgc cgtgaaaatc aaccgcttcg gtcagcagat tgtacgtccc
     5281 attcaatcgc gcatcggcgc cgggaacttt gaatgaaaga ttgctaaacc tggccacgcc
     5341 atcgcgaagg gacacgtgcc cgcgaagatc tgaaattacg ttttcctgcg gagtgcttgg
     5401 attctgcttc tgctcttgag cggttttctt gtctccccga gccgtcgcgc tcaggtcatc
     5461 cgctttagct tgcgtctgcg gtgtttcgaa gtgcccgcct ccgattccaa aatcgccgtc
     5521 cagagtcact tcttttaaga acggctttcc ctcgggcgga acagtcacgt gagcctgaaa
     5581 gctggtaacg cccgccatcg gaggttttag tgcgtggacg aagagttgca gcacatcttg
     5641 aatgcgccct tcgctgacgg cgaaatcgag cgcggtgaat ttcgacctat aatcgggttt
     5701 cttcgctacg ctcccgttca cggcaatctt cgtcttcccg tagaaagcgt tcacccggct
     5761 caacacaata tccccattca gcgcatctac gccgatctgg aactgggtgg aaagacgcgc
     5821 cgcatgtccg ctacgcgcga cctcgaaatt cggggcgtcc gtgtagcctt gcgcgtttat
     5881 atgcgctaaa tcgcccgaaa aagttccgct cgagctgatc gtgccggcca cgccctgaaa
     5941 ggcgcccaaa ttggctcgct cgaaggaata cgttccggat agttgcgtct tgctcggctc
     6001 ctgtgagttg aacgggccaa aagaacctgc ggattggatc tcgccgggag gctcgggatt
     6061 gtgcatgtcg acgcggtagg aaaatgcttg cttcgcgccg acagatttca ggtcgagctg
     6121 gtgaatctca aaccgtaaag gcggccgatt attcgcgcgc gcaatttcga acacggctcc
     6181 gttcgcgatc gcttcaccaa tcgtgatttt ggaagttgag gtcccgccgc cgaaattgcc
     6241 gccatggccg ctttgcggga tgtggacgcg gaggccctcc aaaaccatac gagcgatgtg
     6301 atgcggcttt atcagaagat ccggataact tcctagtatg gtcaacttcg aaaccgtgac
     6361 aagaggcggt gaattcggtt cgctcgattt ggaacgaaaa gtgaggcctt ccactatgca
     6421 accggggcga ggaaagtata ctgccctaaa atggttcacc gtaagcgtac tcggcagagt
     6481 ttcgcgcaac gactcaccca cacttctttt cgagtaagga aaatataccg ccagcagaat
     6541 cgcggctact aacaacacca cggcaacgcc cgcaattcct tttagccatc gccggcggac
     6601 gcgccttgca ttggacggag tcgtcgtcct atctcggtga gcggctgatg agtcgctctc
     6661 taagttcgtc acgtaagccc cttgcacacg ttcttcaaca acacacatcc agatttcatc
     6721 ggggtgcaag atgtgtccat caggttgact tggtattcgg ggggcggttt taattggggt
     6781 cgtccgaccg ttcaccgtct ttgtgggtgg ccaaatatcg agtaaatgta acttgcattc
     6841 tgcaagcgat atttggtatc gtccccgccg tggtagggaa gtcccagtca aagcatcggt
     6901 cggcttgtcc cgtaagtatt tcgctcgaga tgttgggaga tcgctggtcg ctgctgatca
     6961 ttcgcgatct gatggttcgc ggctatcgca cattcaagga attccgggga tcaggcgagg
     7021 gaattgccac caacattctg tcggatcgtt tgcgcaaact ccaggcggtc gggattatca
     7081 ccgcgggagc cgagaagacg gacgcgcgca aggtgaacta ccggctcacg gaaaagggaa
     7141 ttggcctcgc cccggttctt ttggaattgc tcatttgggg agcgcgccat gaagagacgg
     7201 gagcgccctg cgccgtgata gacgaaatgg aaaagaatcg ccagcaagtc cttgctgaaa
     7261 cgcggcgccg ctggcaggaa caagacccga ctcccatact accgaaattc gcggatcgcg
     7321 agggccatcg caatttgacg aaagcacgcc ggggtgccta acttaggaaa tagtaagaca
     7381 gctcgtcacc atcagtagcg gcgagcatca aacacttcaa tccagccaaa ctccaaggag
     7441 cgagccatgc agctaaatgc gtatctcaat ttcaacggcc aatgtgaagc ggcttttaag
     7501 ttttacgaga agtgtctggg aggcaagatt gaggccatgg cccggttcga ggacatgccg
     7561 gaaggcggtg gcgtgtcacc ggaatggcgt agcaaaatca tgcacacgca actaaacgcc
     7621 gccggcacgg ttttgatggg ctctgacgct ccggacggtc gctaccagaa gccccaggga
     7681 tttgccgttt ccctccagac cagcgatccc gccgaagcag atcgtgtttt taacgctctc
     7741 tccgaaaatg gcgccgtcag cctgccgatc caggaaacgc cctgggctgt tcgcttcggt
     7801 atgctcgtgg accgctatgg cattccttgg atggtcaact gccagaaagc ggccgcggcg
     7861 gcaccggccc agtcttgacc gatttataac cacacaagcg gaaatatcca cacgtcgaaa
     7921 ggggaaagga tgagcaaagt acgcgtattg gttggcacac gaaagggcgc gttcattctt
     7981 gagtcagacg gcaaacgtga aaaatgggaa gtaaagggtc cccattttgc cggctgggaa
     8041 atgtttcacc tgaaaggatc gccggtcgat cccaatcgaa tttatgcttc ccaaactagc
     8101 ggctggtttg ggcaagtgat tcagcgttca agcgacggcg gtaaaacctg ggaaactccc
     8161 ggcggagaaa aaatgcccac gccgggcggt ccgcctccgg gcaagagtaa ccatttcctc
     8221 tacgacgctt ctccagaaac cggaaagcct ctcaccacgc atcagtggta cgacggcacg
     8281 cagcatccct gggagttcaa gcgcgtctgg cacctcgaac catcgcccac cgatccggac
     8341 accgtctacg ctggcgtgga agacgccgcg attttcaaat ccacggatgg cggcaaatcg
     8401 tggaacgaac tctccggact tcgcgggcac ggcaccggtc cgaaatggca gccgggcgcc
     8461 ggcggaatgt gtttgcatac catcgttctc gatcccagca atcccggacg aatgtacatt
     8521 gctatttcag ccgctggcgc ttttcgcacc gacgatggcg gcaaaacgtg gaagccgatc
     8581 aaccgcggac taaaatccca atatatcccc gatcccaccg ccgaagtcgg ccactgcgtc
     8641 caccgcatcg ccatgcacaa gtcacgcccc gacgtactct acatgcagaa gcactgggac
     8701 gtgatgcgca gcaacaacgc cggcgaccag tgggaagaag tcagcggcaa cttgcctacc
     8761 gatttcggct tcgtgattga cgttcacgcg cacgagccgg aaacaattta cgtcgtgccc
     8821 atcaaaagcg attcggaaca ctttccgctc gatggcaagc tccgcgttta tcgcagcaaa
     8881 accggcggaa acgaatggga ggtcctctcc aaaggcctgc ccgatcgcga ctgctacgtc
     8941 aacgtgctgc gcgacgccat ggccgtcgat tcgctcgatt cctgcggcat ctatttcgga
     9001 accactggcg ggcaagtgta cggttctgcg gacgcgggaa atagctgggc gcccatcgtt
     9061 cgcgatcttc ccgccgtgct ttccgtcgag gtgcaaacac tgccatgatc ctcgtcgtgc
     9121 ttccgccgca tttgcgaacg ctggcgcgcg tcacgggaga tgtggagctg gaggtcgcgg
     9181 gtcaacccac ggtgcgttcc gtcctcgacg cactggaggc ccgctacccg atgctctgcg
     9241 gaacgattcg tgaccaggcc acaaaagagc gccggccctt cctgcgattc ttcgcctgcg
     9301 aaggcgatct gtcgcacgaa tcgccggacg ctccgttgcc cgaggccgtt gcctcaggca
     9361 aggaaccctt tttcatcatc ggcgcgattg ccggcggcta aactcgctgc gaatacgttt
     9421 gctttcgcat ccaggcccgc gcgtgtccct ttgactcgtc atcctgggcg gagtcccgcg
     9481 cttttttctt cgcgcggttt ctgcgcggcg cgggacgcag tcgaaggacc tctcttcgat
     9541 tgccaatccg ccccggtgga caagcgcggt gcgctagtct ttccgtcgga gatccggcct
     9601 tcagtcctaa cacacaaacg ttcgaagcgc agtccttacg ccgcgtaaaa caaaggtcta
     9661 gctcggaaat tgcgcaagcg tagtgcgaaa ggcgctgttg gccatttcct cgagcggctt
     9721 cttgcgcagc tccgcgttca gcacttcaat tccccacggg ccgtcgtacc ctgccttcag
     9781 catcttcgaa acgaaatcct tcggattgaa ttccccctcg ccgcagagca atcggtgatt
     9841 gatcgtatcc tcatgcaggc tccaggagca ctcgtgcgtg ccgtcattga tctcgatgct
     9901 cgtcacgtat ttaaggggaa tgctcgccac tctctcatag ggaattttca gcttcgcgag
     9961 atgccacaga tccagacaga tgccgccgtt cttcgctccc gccccctcga caagctgcaa
    10021 agccttttcc accgagtcga tgtcgcagaa tggcatcagt tcaaagccga ttggtgtgcc
    10081 gtggttggcc ccgtcagcac agagcgccgc gaacgactcg atcattctcg gcatggtggc
    10141 atctgttttg aaaaaatgcc ccactttgat gtgatgcggg cgcagaacgt cggcagcttc
    10201 cagaagtttc ttgcgttcga tgtccgatgc tttcttctct tctccatcga gaaaccagcc
    10261 gccgagaaat tcaagctcca gatgccggat gccgttgtcg tcaaggattt tcttcatctc
    10321 tttcaagctg cgcttcttca gcgtgtgctc caggtccgcg tgccagattc cgacgccttt
    10381 gaaacccacg cgagcaatcg tttcgacccg ctccttgaaa tccacggtgc tgaattcatg
    10441 gttggtgtgc ggctcggcgc ccaccgccag cgtccaatac gatcccagca actcgatatt
    10501 cttattcact ttgtgatggc tccaatcaag cgcgctcgaa atgcacgcgc cgcatgctat
    10561 gcccagcgtt ggtctgcgtc aaccaaacag tattcaccgg ctctgcaaat gaatccgagc
    10621 gatgttattc tctgaagctc atttcgaagg gagctaaaac tcatgcaact cgcggggcaa
    10681 actgcaatcg taactggagg cggccgtggc atcggccgcg ccatcgcgcg caggttcgcg
    10741 gcggaaggtg cagccgtagt ggtaaccgcg cgcacggaaa gcgaagtccg cgaggtcgtc
    10801 tcggaaattc aaagcgcggg cggcaaagcc gcggctatca cagccgacat ttcacacgag
    10861 cccgactgcg agaaaatcgt ccgcggcgcg cgcgaagcct tcggcgcaat ccacatcctc
    10921 gtcaacaacg cgggaatctt cggcccggtg cgccctgtgg aaaaaataag cgcaggtgaa
    10981 tgggacaccg ttctagccgt gaatctgcgc gctcccttcc tgctcgcacg cctcatcttg
    11041 ccggaaatgt acgagcgcgg ctccggcgcc atcgtcaaca tctccacggt tggcgccaag
    11101 gccgcgtacc cgatgaattg cacctacgcc gcgtcgaaag ccggcctcga tggtctcact
    11161 cgcactctcg ccgctgaatc cgcacgcaaa ggtgtgcgcg tcaacgccat ctgccccggc
    11221 ccggtaaccg aaaccaaaat gtcgaaggat ctgacgcgag aattcgcaaa acagctcggc
    11281 tcaaatggag atgaagtttt gaatcgaatg attgcaggaa ccttgcaggg aagaccgcag
    11341 acagcggatg aaatcgcctc cgccgcttta tttcttgttt ccggccaggc tagcgcgatt
    11401 actgggcaga ccctgaatgt tgatggcggc cttgtctttt actgatggat tccatcacgc
    11461 cgttgctaac gtattccagc gcgccgtacc cgcgtcgcgc agtggtggca attgcgcgca
    11521 ctcccacttc gccccaaacc acgccgtatt cccaggcgct tcccagcgtg taacaaatcg
    11581 cgacaaacaa aaatatcgcc gcgaatacta tcccgaaaag caactccgcc gcgatcaact
    11641 cgcgcacgaa gtaagcctgc aaaatgacga tta
//
LOCUS       DATNDT010000412         6944 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1273812, whole genome shotgun sequence.
ACCESSION   DATNDT010000412 DATNDT010000000
VERSION     DATNDT010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6944
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1273812"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VN788_13430"
     CDS             complement(<1..1148)
                     /locus_tag="VN788_13430"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU21579.1"
                     /translation="MDQTLDEELSSYRQLLEDEKIAAGVDSQTAQREAAIELGGIEVI
                     KEQVRDIRRGAFLEGFWTELRQSIRALRRNPGMTAMCVVMLALGIGASTIIFSVFEAA
                     LLRPLPFRDAERLVEFVETQLDLGMVEQNLSEADFWDLRDRTKSFESVGAYNGFQANL
                     TGDGEPEKVSGAQVTLEFFRTLGVNPILGRDFSAGEGNANIVILGNRFWKARYGADAN
                     VLGKVLQMKGISYTVVGVLPRGEPWIDGQVYLPYSYHPAADRGNREFAAVGRLKRGVT
                     AEAARRELQRLAAVMRAADPKEDKGLGFNIFPSSRWIAPEETGTALRMLLVAVGLLML
                     IACGNVTNLLLARGLARRREIALRSALGAARARLVRFVMLESLLLSALG"
     gene            complement(1205..1564)
                     /locus_tag="VN788_13435"
     CDS             complement(1205..1564)
                     /locus_tag="VN788_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXU21580.1"
                     /translation="MPIENLYETPPDANIALLQGTLDVLILKVLALQPMHGLGISRRI
                     AQVTNATFQVKPGSLFPALHRMEEAGWLAAQWGESENKRRAKFYRITKIGVRKLKQET
                     DDWRRISAAMAVALEIP"
     gene            1773..3002
                     /gene="istA"
                     /locus_tag="VN788_13440"
     CDS             1773..3002
                     /gene="istA"
                     /locus_tag="VN788_13440"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HXU21581.1"
                     /translation="MDEINQLKREGLSILAISNLTGYDRKTIRKYLLEPTSKPEYPPR
                     PKPAGKLDPFKVYLDERLQTGVWNARVLLRELRSRNYTGGYTLLTDWLRPQREAARVI
                     AVRRFETGPGKQAQVDWGHLGSLTEGGEAQAMWGFTMTLGYSRRMMAEAATNQKLGTL
                     LRMHEAAFEELGGVPEEILYDRMKTIWTGTDERGEIIWNAVFLDFARYWGFRPRLCRP
                     YRAQTKGKIESGVKYVRRNFLCSLLGREPGSLVELNAELRRWISEVANQRVHGTTHKQ
                     VLARWGEERGALQSVNGRPAYPFRDDEQRKVARDAYVSWQGSRYSVPWQYAGKDVWVR
                     EHDGGVEVRYGAERIAAHEPAATRHQVITLDEHHQGIPLGSPRAGKTLIHIQQSAPVV
                     EQRPLEAYESLAMGGTR"
     gene            2999..3817
                     /gene="istB"
                     /locus_tag="VN788_13445"
     CDS             2999..3817
                     /gene="istB"
                     /locus_tag="VN788_13445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HXU21582.1"
                     /translation="MSTIERLRTTLTALNLTAIDARLEALLESASKKDPAYADFLLEV
                     MSTEADARRQRYLKTRLQLAHLPYIKTFDQFDFAFQPSIDERQVRELRTLRFVHEASN
                     VILLGPPGVGKTHLAVALAETAIQSGQAAYFMTAHDLVTDLGRAYREGRLDRRLRIYL
                     APKVLIIDEMGYLPLDELGATIFFQLVSARYERGSIILTSNKSYGEWGTIFGDPIIAT
                     AILDRLLHHSTTVNIRGESYRLKERRKAGLIPMPESQQDDSRANAPSGRGKRTL"
     gene            complement(5383..5874)
                     /locus_tag="VN788_13450"
     CDS             complement(5383..5874)
                     /locus_tag="VN788_13450"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HXU21583.1"
                     /translation="MSDARGPWGAEDVLENTRRCTPAFFEELVVDLLIAMGYGGPNGE
                     GGRAVGRAGDGGIDGIIEEDKLGLESVYVQAKKWEGTVGRPTVQAFAGGLEGRRAHKG
                     VLITTSEFSLEARDYVHTIGKKIVLIGGQRLAELMIEHGIGVTDGPAYQIKKLDEDYF
                     QGG"
BASE COUNT         1489 a         2010 c         1969 g         1476 t
ORIGIN      
        1 cccagagcgc taagcagcag cgattcgagc attacgaaac ggacgaggcg tgcgcgtgcg
       61 gctcccaggg cactccgcag agcgatttcg cgccggcgcg ccaacccgcg cgccagtagc
      121 aaattcgtga cgtttccgca cgcaatgagc attagcagtc ccaccgccac gagtagcatc
      181 ctcagcgcgg tgcctgtttc ttccggggcg atccagcggc tagacggaaa aatgttgaat
      241 ccgagcccct tatcttcttt gggatcggct gcccgcataa cggcggcaag gcgttgcagc
      301 tcccttctcg cggcttcagc agttactcct cttttaagcc gccccaccgc ggcgaattcc
      361 cgattcccgc ggtcggccgc cgggtgatat gagtacggca aatagacttg accatcaatc
      421 cagggttcac cgcgaggcag gacgcccacg accgtgtagc tgatgccctt catctggagc
      481 actttgccga gaacgttggc atcggctccg tagcgcgcct tccagaatct attaccgagg
      541 atgacgatat tggcattgcc ttctccagcc gagaagtccc ggccgaggat aggattcact
      601 ccaagtgttc ggaagaattc aagcgttact tgcgcgcccg aaaccttctc gggttcgcca
      661 tcgcctgtga ggtttgcttg aaagccattg tatgcgccaa cactttcaaa cgattttgtt
      721 cgatcacgaa ggtcccaaaa atcggcttcg gaaaggtttt gctcgaccat cccgaggtcg
      781 agctgcgtct ctacaaattc aacgagccgc tccgcgtccc ggaatggcaa gggccggagc
      841 aacgccgctt cgaaaacgct gaatatgatc gtgctggctc caatgccgag agcgagcatg
      901 acgacgcaca tggcggtcat tcccggattg cggcgcagtg cgcggatcga ctgacggagt
      961 tcagtccaga agccttccag gaaggccccc cggcggatat cacgcacttg ttccttgatc
     1021 acttcgattc ctccaagttc aatggccgct tcgcgctgcg cggtttgcga atcgacgcca
     1081 gcggcgatct tttcatcctc aaggagttgg cggtaagaac taagctcctc gtcgagagtc
     1141 tgatccaccg cacgcttgtg aaataaattg cgccagattg ttatgagcga ccgcatggaa
     1201 gtccctacgg gatttccagg gcgactgcca tggcagctga gatgcggcgc cagtcatccg
     1261 tttcctgctt cagctttcga acgccgatct tggtgatgcg ataaaacttg gcgcgacgct
     1321 tgttctctga ttctccccac tgcgcggcga gccagccggc ttcttccatg cgatgaagag
     1381 caggaaacag cgagccaggc ttcacctgaa atgtggcatt cgtcacttgg gcaatgcggc
     1441 gggagatacc gaggccgtgc atcggttgga gggcgagcac tttaagaatg aggacatcga
     1501 gggtgccctg cagtagggcg atgttggcat cgggaggtgt ctcgtataga ttctctatag
     1561 gcatcctaca gagattctat atgagctctg gcgaaactgc aatcacgcgc gatcggcccc
     1621 aatcgcgttt caagacaagg cagacgtact gtcagcgcca gtgcaataat ccccaaagtg
     1681 ccggttgaaa attccccacc tcctcagaca ggtggtgtta accccgactc tggaatacag
     1741 gggagggccg acatctgttc agggggaaag acgtggacga gatcaaccaa ctcaaacggg
     1801 aaggactcag cattttggcg atcagcaatc tgactgggta cgaccgcaag acgatcagga
     1861 agtatttgtt ggagccgacc tccaagccgg aatatccacc gcggccgaag ccagcgggca
     1921 agttggaccc attcaaggtg tatctggatg aacggcttca gacgggcgta tggaacgcga
     1981 gggtgttgct tcgagaactc cgctcgcgca actacaccgg gggctacacg ctgttgacgg
     2041 attggctacg tccacagcga gaggcggcgc gggtgatcgc cgtgcggcgg ttcgagactg
     2101 gaccgggaaa acaggcgcaa gtggactggg gccacctggg gtcgctcact gaaggaggcg
     2161 aagctcaagc gatgtggggc ttcaccatga cactgggcta cagccggcgg atgatggctg
     2221 aagccgctac gaatcagaaa ctcgggacgc tgctacgcat gcacgaagcc gcctttgagg
     2281 aactgggggg cgtcccggaa gagattctct atgatcggat gaagacgatc tggaccggga
     2341 cagacgagcg cggcgagatc atctggaacg cggtgtttct ggatttcgcg cgctattggg
     2401 gcttccggcc gcggctgtgc cggccttacc gggcccagac caaaggcaag atcgaatcgg
     2461 gtgtgaagta cgtgcggcgg aattttctat gcagtctgct gggccgggag ccgggcagcc
     2521 tggtggagct caatgccgag ctgcgccgct ggatatcgga ggtcgctaat caacgagtcc
     2581 atggcacgac tcataaacaa gtgctggctc gctggggtga ggaacggggt gccctgcaat
     2641 cggtcaacgg ccgaccggcc tatccgttcc gcgatgatga gcagcgtaag gtggcgcgtg
     2701 atgcttacgt gtcctggcaa ggaagccgct attcggtgcc atggcaatat gccggcaagg
     2761 atgtttgggt tcgcgagcac gacggcgggg tagaagtgcg ctatggagcc gaacgcattg
     2821 ctgcacacga accggcggcg acccgccatc aggtcatcac gctagatgag caccaccaag
     2881 gcattcccct cggcagcccg cgcgcaggca aaacgctgat tcacattcag cagagcgcgc
     2941 ccgttgttga gcagcggccg ctggaagcct atgagagtct ggcgatggga ggcacgcgat
     3001 gagcaccatc gaacgattgc ggaccaccct taccgcgctc aacctgactg ccattgacgc
     3061 gcgactggaa gcgctgttgg agagtgcttc gaaaaaggac cccgcttacg ccgacttcct
     3121 gcttgaggtc atgagtacgg aagccgatgc gcggcgccag cggtatctca agacgcggtt
     3181 gcaactggcc cacctgccgt acataaaaac tttcgaccag ttcgacttcg ccttccagcc
     3241 ctcgattgat gaacgtcaag tgcgggaact tcgcacgtta cgcttcgtgc atgaagcgtc
     3301 caacgtgatt ttgttgggac cgccaggggt cgggaaaact catctggcgg tggcactggc
     3361 agaaacggcg atacagtccg gtcaagcggc ctacttcatg acagctcacg atctggtgac
     3421 tgatctgggg cgggcgtatc gggaaggacg cctcgaccga cggttacgaa tctatctcgc
     3481 acccaaggtg ctgatcatcg acgagatggg ctatctgccg ctcgatgaac tcggcgcgac
     3541 gatcttcttc caactggtta gcgcgcgcta cgaaaggggc agcatcatcc tgacttccaa
     3601 caagagctac ggcgaatggg gcacgatctt cggcgacccc atcatcgcga cggcgatcct
     3661 cgatcgcctg ttgcatcact ccaccaccgt gaacatccgc ggcgagagtt accggctcaa
     3721 ggaacgccgc aaggccggct tgattccaat gccggaatca cagcaggacg actctcgggc
     3781 taacgccccg agcggtcgcg gcaagcggac cctttgagtt tttaaggcat gggcaaatct
     3841 atcaatccga tgtgctgttc tgttaacgcc cagggcgagg ccattgcggg ctccgccccg
     3901 gatcccatcg tcttgatgag tttgcagtcg gctattcctt ggcggggtgc actcctgcag
     3961 tgcccgcctc cgcttcaccg gctggtttct atttaccatt caccagccaa aactgtaaac
     4021 caccacctca cgagggctgg ggaattttcg accggaataa tggggaattt tcaaccggta
     4081 ttgacagtac agctctcgcg cattattccg ggatcgattc caggatggtc ggcaagcagg
     4141 aagcaatcca tatgagcgga gtcgcctttg ctgggactgg ccagaccggc atttcggcga
     4201 ttgctccctc ctggttggct actcggtagt gatccatgtg gcggcggagt cgccgtcccg
     4261 gactttggcg cggcgattcg gtcaaatccg gtgttccggg cgacagatcc tgccgagtcg
     4321 accaccgttt accgatccag tgttctgccg gttcgccttc ggtcgtgcgt tgtcagaatc
     4381 gccggccaca ttatgattca aaacggccat tttatggttc aagtcacatt gccgtcctta
     4441 cgccggtttg tgctgcgcag aaacataact caagcagcag catgccagag ttggctttct
     4501 cagaaaagag gcaaggtctt cacctaaact gttttcgccg attttcgcag tgccggcgtt
     4561 gaaaacaaaa ggaattttct ggcgccaaga tcgaaaatcc catttaagtg cgaaagtcgg
     4621 gttagactac gcagagccgg ctgaccactc gcgatcttac gcgatcagcc gtgttgcttt
     4681 cccccatgag gatagggtca gcgtctctga tcgatccttt tcggagctca atagcccggc
     4741 ccaccgatgc ccttgtctac gcttcgaagg aaacctcacg atctcccccg caagactcag
     4801 ggtcaggatg gagtcgcttt ctcctttcct gcagggattc ttcctcccct gcaacatgcc
     4861 ggtttatccc ggcgcacccc caaacaagac tcgaacctgc aacccttccg ttaacagctg
     4921 agggaaagcg aatgcccgaa tattggtcat tccgcgctat attcgacgct ctagccgttc
     4981 cccgaatggc ggatcgccag cgcgattccc ccggaacgaa aacccgtcga gaaagatcat
     5041 ccccggctgg ggccggttaa ggcgatcatc gaccggattc tggaagagga cccccgtccc
     5101 ttaccgcgct ccgaaagagg tgttcggttt caggtaaggc agcagtgaaa tccggcgtat
     5161 tgagaactcg ccttgcatct tcccccggat ctcttttacc tttgctccat cacccctaga
     5221 ggggtcataa agcgcttcgc tgatcacgac gtgctgatca ccccactagt cgtaaggcta
     5281 cggggccgtc gctgtaagat tctgatcgcg cgcgtcgttg cggtacgcgc gagtgagcat
     5341 cggactttcc tccaattaac aaaacatcga aataatcggg cttcatccgc cttgaaagta
     5401 atcttcgtcg agcttcttga tctgataagc gggtccgtct gtcacaccta ttccgtgctc
     5461 aatcatcagt tcagccagtc gctggccgcc gattagtacg atctttttgc caatcgtgtg
     5521 cacataatcg cgcgcttcga gcgagaactc gctggtggtg atcagcacgc ccttatgcgc
     5581 gcgccgcccc tccagacctc cggcaaatgc ctgtacggtg ggtcggccaa ccgtaccttc
     5641 ccacttcttg gcctgcacgt acaccgattc aagtcccagt ttgtcctctt cgatgatgcc
     5701 gtcgatgccg ccgtcaccgg ctcgcccgac tgcacgtccg ccctccccat tcggaccacc
     5761 atagcccatg gcaatcagca gatccaccac gagctcttca aagaatgccg gagtgcacct
     5821 tcgcgtattc tctaaaacgt cctcagcgcc ccagggacca cgcgcatccg aaatcgaagc
     5881 cgcttaccat atcctttcta atacgatgga aagcttactg ccaggaacgg aggtacaggc
     5941 gcgcggtctg cgctgggagg tgatcttctc taatctcccc gaaatatcga tttggagttt
     6001 ctaaatctaa tggtagactc cgagtcgggt ccactttatc ccacgccttt acgttgtgac
     6061 tttggaaagg acagataaaa atcgcgtgcc tatgcttgct ggggatcatt ggggaatagc
     6121 cattcgtgct ttgctacagt gcagcgcggt cacgaccgct gcaatgttga ttaatcggct
     6181 tgcaattcgg aaaccgcgtc ctcccctttc acgacgatcg agtctggcgg tcgatgcgct
     6241 actgggagca gctggcttct gggccgtgaa tatgtggttc caagcgccga ttgaagcagg
     6301 gttgtggggg acgatatttc ttacctacat actggacgac ttgatttgcc ggctcatcct
     6361 tcgctaggga ggccgaagtg cgcggagaaa gctgccgaac atcaaactca agcgctcgat
     6421 tggctgcctg tcaaaagccg aaaaagtcgc ggccgaagta attgttatcg atcacgtaca
     6481 aaaaggccca ggggaaaact aagaacattt gcctcgccaa acaagactcg aacctacaac
     6541 ccttccgtta acagctgagg gaaagcgaat gcccgaacat tggccatccg cgcgacttcg
     6601 accagcttgc aggatccggt ttgccacgtc ctgggtctcc ccttgcaagt tctgatgccc
     6661 ttaactgaag ggttgcagtt tcgagtcttg tttggtgagg caatattttt catcacgcgc
     6721 cgtgcggctt tgtaatcagc agccactctg gagcgacagt taccttgcat ttggaagggt
     6781 ctggtcccgg cagcgatgtc cagcgcccac gcgcccgtgg gaaacatttc tcaaacggac
     6841 cagcaactct atttcggaac cggcgggtcc gcgaatcggg tgctggcgac gggcgacgcg
     6901 ctgtttggta caacggtgac caaagtctcg attgggacga aatc
//
LOCUS       DATNDT010000413         5792 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_675869, whole genome shotgun sequence.
ACCESSION   DATNDT010000413 DATNDT010000000
VERSION     DATNDT010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5792
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_675869"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="VN788_13455"
     CDS             complement(<1..164)
                     /locus_tag="VN788_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU21584.1"
                     /translation="MDLQLSGKLALVTGASKGIGRATATTLAEEGCNLVLVSRDLAAL
                     NKVAEAISLR"
     gene            374..571
                     /locus_tag="VN788_13460"
     CDS             374..571
                     /locus_tag="VN788_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21585.1"
                     /translation="MAKNNRLTKAAVKIGAAMGKADRTAHKVARAGVLAKSELEDISR
                     QIDSLKRQLLKTTKRLKKALS"
     gene            complement(613..1002)
                     /locus_tag="VN788_13465"
     CDS             complement(613..1002)
                     /locus_tag="VN788_13465"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXU21586.1"
                     /translation="MTIRSASELYLVDSSGWLEYLTEDSKSASFGNYLEGDATVLVPS
                     VVIFEVYKQLSKHRGKSIADRFASQALMRKVVPLDETIALAAANVSLEHRLSGADAIV
                     YATARVWQAQLVTANTRFRGLPAVIIP"
     gene            complement(980..1198)
                     /locus_tag="VN788_13470"
     CDS             complement(980..1198)
                     /locus_tag="VN788_13470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006853833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HXU21587.1"
                     /translation="MEKATVRVSPKFQVVIPKLIREELKIEPGQQLMMYAIDGSLRLV
                     PHRSVKELKGIAKGMRWKEEYRDHSERF"
     gene            1657..2325
                     /locus_tag="VN788_13475"
     CDS             1657..2325
                     /locus_tag="VN788_13475"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXU21588.1"
                     /translation="MGRFWGGVIVGLIIIPVVAAVYLLTGLAPAAVSDHPFPFERLIA
                     GAALESRVHREAPKRDLATFTTADLMAGAQTYRKGCGCHGSLEKETYPEPQMYPPPPH
                     LLMPDGYATDDSVGETYWRIKNGIRMTAMPSFKTVLTDDQMWQTAALVARADKLPPEV
                     LDVLKQPLLPSPETPPAAAPRGSNSNPNPVPPAPTHGVAPGSQITPKNVRPMTGAHTP
                     KTPK"
     gene            complement(2429..3991)
                     /locus_tag="VN788_13480"
     CDS             complement(2429..3991)
                     /locus_tag="VN788_13480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HXU21589.1"
                     /translation="MLDLIKNGNSALAVPADADEGQIIPEYFASRLFRLPIARANLAA
                     ALLAGLALRLFFVIRFSFFTGDTKFYDALARNWLYHGVYGLFVAGQLTPTDMRAPGYP
                     AFLAAVYALFGPNHRAVLIAQVIVGLLTCLGIAAIAARLAPTASRKKAATAALWIAAL
                     CPFTANYASILVTEELAMLFTTFALLIFVSLLEHPFIASPNSASPRSTFSFAGWAAIG
                     GILVGLGTLVRPETPLVLAAAGLIFTIRLRRRIDWPKLTLTISWMAVGLLLPLLPWAS
                     RNARVLGRIQFLAPHYAETEGDFIPYGFFNWSKTWMTRPDENYLVPWKLGNAPIPIES
                     LPASAFDSMAERNRVATLLTAYNADLKMSPLLDHEFELLARERTARHPLRTYLVIPLE
                     RVGAMWFTPPVRALRYSGALWPPGERWRENPWDFGITAGFGLLNLVYFGLAVAGAWHC
                     RARSAMAMLIAFVLIRTALLTQQTSIEPRYVILCFPAFLAIAAQAWTIARPSLATSAA
                     RSGWAAGQATAD"
     gene            complement(4074..5174)
                     /locus_tag="VN788_13485"
     CDS             complement(4074..5174)
                     /locus_tag="VN788_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012977233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HXU21590.1"
                     /translation="MKVRAAVAREGQRLLSIEELDLTGLGPDGILVRIVGVGLCHTDV
                     KTLQGTMAVPKPVVLGHEGAGIVERTGERVSKVKPGDHVVLTFDSCGTCRPCTTGNPA
                     YCAQNMALNFRDVREGAPGSFHKGNETIHGHFFGQSSFANYAITTSRNTIPVRKDAPL
                     EILGVLGCGVQTGAGAVMNSLQPAPGSSIAIFGVGPVGLSAVMAAAVRGCAEIIAIDV
                     LAQRLETAKELGATRVVHAIGKQSTAEEIWRAVPGGVNYALDTTGRAATFEQAIASLA
                     TKGCFGFVTAPTGNPSLNLSAVMIGGHSVRGIVQGDSAPEAFIPYLIELHMSGRFPFD
                     KLVSKYPFEQINQGIADQAIGKVVKPVFTFSS"
     gene            complement(5208..>5792)
                     /locus_tag="VN788_13490"
     CDS             complement(5208..>5792)
                     /locus_tag="VN788_13490"
                     /inference="COORDINATES: protein motif:HMM:NF042649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXU21591.1"
                     /translation="RKRNIANERWMGVLDAVAKAAKWEPRPPASKASSKGASAKIASG
                     RGIGLGTHLTSYGAAVAEIEVNRETGRVVVKHMYGAIDAGLAVNPGFIENQISGQLVQ
                     ATSRMLHEEVTFSKTNVTSLDWNSYPILRFEECPEVTTVVVQRTDQKSTGAGEEVMAA
                     AAGAIAGALFDATGIHMREFPLTPKRVLTALTRA"
BASE COUNT         1273 a         1598 c         1789 g         1132 t
ORIGIN      
        1 aatcggagac tgatcgcctc ggccactttg ttcagtgccg ccagatcgcg tgagaccaga
       61 acgagattgc agccttcctc ggccaaagta gtggctgtcg cgcggccaat gcctttggaa
      121 gcgccggtga cgagcgcgag cttgcctgac aattgcagat ccatgggcga tttcctccgg
      181 aaggccgttc cgaatcggct gcggatttcg tccagccgag aaccgaaaag gttatacctg
      241 aaacagcatc atttcaattt ccgcgcagcc aatgtcgccg gctaggacgg gacctgagat
      301 cgaaacctta ggtaaagaat ttctttgcgg ggcatgcgat actccgggtg caggcgaagg
      361 gagaaaacga aaaatggcga aaaacaaccg gctcaccaag gcggcggtaa aaattggcgc
      421 ggcaatgggc aaagcggatc gcacggcgca caaagtggca agggccggcg ttttggctaa
      481 gagcgaactc gaggatattt cgcggcagat cgattctctg aagcgacagt tgttgaagac
      541 cacgaagcgg ctgaagaaag cgttgagcta aactcctcca atagtccgcg gatggtaacc
      601 gccagccgcg cgtcacggga tgatcacggc gggaagtccg cgaaagcgcg tgttggcggt
      661 gacgagctga gcctgccaaa cgcgggccgt ggcgtacacg atggcgtcgg cgccagagag
      721 ccgatgctcc aggctgacgt tagctgccgc aagcgcgatg gtctcatcca gtggaactac
      781 cttgcgcatt aacgcctgcg atgcgaagcg atcagcgatg gacttcccgc gatgcttgga
      841 gagctgtttg taaacctcga agatcacaac cgaaggcacc agtacggttg cgtcgccctc
      901 taagtagttt ccgaaggatg ccgacttcga atcttcggta agatattcga gccagcctga
      961 ggaatccacg aggtaaagct cagaagcgct ccgaatggtc acgatactct tctttccagc
     1021 gcatgccctt ggcgatgcct ttaagttcct tgacggagcg atgaggcacg agccgcagcg
     1081 agccatcgat tgcgtacatc atcagttgct gtcccggctc gatcttgagc tcttcccgaa
     1141 tcagctttgg gatgacgacc tggaacttgg gcgagaccct gacggtagct ttttccattt
     1201 taggaagctt ctccccggaa atcctatcga tcagcggttc gtatgtcaat aaggctatcg
     1261 atcgaccata tgtaatacgt cgtcggtttt ccccgatgat ccgattgaat ccatgagtgt
     1321 tagtgagccg gaagtttttt cgaaatcgaa tcggaaattt tcgcgccgct agcggcctgc
     1381 aaaattcctg cgatagtggg atgcgagaca ccatttcagt cggcgcgtcg acatcaaaat
     1441 gaaacagcgt ggggagaatt tctcattaaa aatttcacta tgtagggacg ctaaagagca
     1501 tctggccgtg cagggcgatt cggtgtccgg gtgatgacga aagccgtcca ttttggtgcc
     1561 accgattcat caaacaaccg agaagaggag tagacttcgc tgtgcgttcc tgcggaagcg
     1621 gccgggaagt ggagacatga aaggagtggt tgaaaaatgg gaaggttctg gggaggagtc
     1681 atagttggac tgattatcat tccggtggtc gcggccgtgt acctcttaac cggattggcg
     1741 ccggcggccg tctcggatca cccatttccc ttcgaacgcc tgattgcagg ggcggcgctt
     1801 gagtcgcgag tacaccgcga agcgcccaag cgcgacctgg cgactttcac gaccgctgat
     1861 ttaatggctg gggcacaaac gtatcgaaaa ggttgcggct gccacggctc gctcgaaaag
     1921 gaaacgtacc cagagccgca gatgtacccg ccaccaccgc acctcttgat gcctgacggg
     1981 tatgcgacgg atgattcggt gggcgagacc tattggagga tcaagaacgg cattcgcatg
     2041 acggccatgc ccagcttcaa aacagtgctg acagacgatc agatgtggca aaccgcagcc
     2101 cttgtggcgc gcgccgacaa gcttccgccc gaagtcctcg atgttctgaa acagccgctc
     2161 ttgccgtcac ctgagactcc gccagcggcg gctccacgtg ggtcgaattc caacccgaac
     2221 ccggttccgc cggctcctac gcatggggtt gctccgggct cgcaaatcac gccgaaaaac
     2281 gtgcgaccaa tgaccggggc ccacacgccg aaaacgccaa agtgaggccc ggaactgaag
     2341 tgccaggcga aacgtacgcg cagttccctc gaatcgccct agagtctgag gcatgtacac
     2401 caaatgacgc cagaagacgg agcggctatc agtcggccgt ggcttggcca gcggcccagc
     2461 cacttcgcgc cgcacttgtg gccagggagg gtcgcgcgat cgtccaggcc tgcgcggcta
     2521 tggccaaaaa tgcggggaaa cacagaatca cgtagcgggg ctcgatggac gtctgctggg
     2581 ttagcaacgc cgtgcgaatg agtacgaatg cgatcagcat agccatcgcg gagcgcgcgc
     2641 ggcaatgcca cgcgcctgct accgccaagc caaagtagac aagatttagc agtccaaacc
     2701 cagcggtaat tccgaaatcc cacggatttt cgcgccatcg ctcgcccggc ggccacagcg
     2761 cccctgaata acggagtgcg cggacaggtg gcgtgaacca catggccccc acccgttcga
     2821 gggggatgac gagataggtg cgcaggggat ggcgcgcggt tctctcgcga gcaagaagct
     2881 cgaattcgtg gtcgagcagg ggcgacattt tcaaatcggc attgtaagcg gttagcaggg
     2941 tggcgacgcg attgcgttcg gccatggaat cgaaggcgga agcgggcaga ctctcgatcg
     3001 gaatgggggc attgccgagt ttccaaggca ccaggtagtt ctcgtcaggc cgcgtcatcc
     3061 aggttttcga ccagttgaag aagccatagg ggatgaagtc gccttccgtc tcggcataat
     3121 ggggcgcgag aaactgaatg cgtcccagca cgcgcgcgtt tcgtgaagcc cagggcagca
     3181 gaggcagcag caaacccaca gccatccagg aaatcgtgag agtcaatttc ggccagtcga
     3241 tgcggcggcg caagcgaatc gtgaatatca gaccggcggc tgccaggacg agcggcgtct
     3301 ccgggcgaac gagagtcccg aggccaacca agatgccacc tattgcagcc catcccgcga
     3361 acgagaaggt gcttcgcggc gatgccgagt tgggactcgc aatgaaggga tgttcgagca
     3421 gcgagacgaa aatcagcagg gcgaaagtgg tgaaaagcat ggcgagctct tcggtaacca
     3481 aaatcgaggc gtaatttgcc gtgaagggac ataacgccgc aatccacaac gccgctgtcg
     3541 cggctttttt tcgcgaggcc gttggtgcga ggcgcgcggc gattgccgcg atgcccaggc
     3601 acgtgagcaa cccgacgatc acctgagcga tgagaaccgc tcgatggttt ggcccgaaga
     3661 gagcgtaaac cgccgcgaga aacgccgggt atccgggcgc gcgcatgtcg gtgggcgtca
     3721 attggccggc gacgaagaga ccatagacgc cgtggtagag ccagttgcgg gcgagcgcgt
     3781 cgtagaactt ggtgtcaccc gtaaagaatg aaaagcggat cacaaaaaag agtcgcagcg
     3841 ctagcccggc caaaagggcg gcggcgaggt ttgcacgagc aatcggcagg cgaaatagac
     3901 gagaggcgaa atattccgga attatctggc cttcgtctgc atcggcggga acggccagcg
     3961 cggaatttcc gttctttatc aagtcgagca catcagctcc gagtttaggc tggcatgagg
     4021 cggcgctgat tgcaatcggt tgttgatgcg aaccgaacaa ccgatgctga cacttacgag
     4081 gagaaagtaa agacgggttt cacgactttg ccgatggctt ggtcggcgat gccttgatta
     4141 atttgctcga agggatactt gctgacaagc ttgtcgaagg ggaagcggcc gctcatgtgc
     4201 aactcgatca gataggggat gaaggcctcg ggcgcgctat cgccttggac gattccgcga
     4261 acgctgtggc cgccgatcat cactgcggat agattcaagc ttggatttcc cgtcggcgcg
     4321 gtcacaaagc caaagcagcc ctttgttgcg agcgatgcaa tcgcttgttc gaaggtggcg
     4381 gcgcgacccg tggtgtccag cgcatagttc acgccgccgg gaaccgcgcg ccaaatttct
     4441 tccgcagtac tctgttttcc aatggcatgc acaacgcggg tggcgccgag ttccttggcc
     4501 gtttcgaggc gttgcgcgag aacgtcgatc gcaatgattt ccgcgcagcc gcgtacggcg
     4561 gcggccatca ccgcgctgag tcccacagga ccgacaccga atatagcgat agagctgccg
     4621 ggcgcaggct ggagagaatt catcaccgct ccggcgccgg tctgaacgcc gcagccgagc
     4681 actcccagga tttctagcgg agcatctttt cgtacgggaa tcgtgttgcg cgaagttgtg
     4741 atggcgtagt tagcaaagga tgattggccg aagaagtgtc cgtgaatcgt ttcgtttcct
     4801 ttgtgaaaag atccgggtgc gccctctcgc acgtcgcgga aattcagggc catgttttgt
     4861 gcgcagtacg cggggttgcc ggtagtgcag ggccggcagg tcccgcagga atcgaaagtg
     4921 agcactacat gatcgccagg cttaaccttg ctgacgcgtt cgcccgtgcg ttcgacgatg
     4981 ccggcgcctt cgtggccgag cacgaccggc tttggtacgg ccatcgttcc ttgcagagtt
     5041 ttcacgtccg tatggcagag acccacgccg acgatacgga ccagaatgcc atctggccca
     5101 agaccggtga ggtcgagctc ttcaatgctg aggagccgtt ggccttcacg cgccacggca
     5161 gctcgtactt tcatgatttc cctctcagcg cgaccaacga catgcagtta ggcacgggtg
     5221 agggcggtga ggacccgttt cggggtgagc ggaaattcgc gcatgtggat tccggtggcg
     5281 tcgaacaaag cgccggcgat ggcgcctgcg gctgcggcca taacttcttc acctgcgccg
     5341 gttgattttt gatcggtgcg ctgcaccacg acagtcgtca cctcggggca ctcctcgaag
     5401 cgcaggatgg gatagctgtt ccaatcaagg ctggtgacat ttgttttgct gaaggttact
     5461 tcctcgtgga gcatgcggct ggtggcctgg accaattggc cgctgatttg attttcgata
     5521 aatcctggat tgacggcgag gccggcgtcg atcgctccgt acatgtgctt caccaccact
     5581 cggccagttt ccctgttcac ctcgatttcg gcgactgcgg cgccgtatga cgtaagatga
     5641 gtgccgagtc cgattccgcg gccgctggct attttcgcac tcgcgccctt tgaagaagct
     5701 ttcgaggccg gaggacgcgg ctcccatttt gcagctttcg caaccgcgtc gagcacaccc
     5761 atccatcgct cgttggcaat attccgcttg cg
//
LOCUS       DATNDT010000414         7994 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2382746, whole genome shotgun sequence.
ACCESSION   DATNDT010000414 DATNDT010000000
VERSION     DATNDT010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7994
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2382746"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /gene="rplS"
                     /locus_tag="VN788_13495"
     CDS             <1..276
                     /gene="rplS"
                     /locus_tag="VN788_13495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HXU21592.1"
                     /translation="DKERIQPFEGVVMSRRGELLGESFTVRRVSFGIGVERIFPVHSP
                     MISAVEVVRQGRVRRAKLNYLRQRKGKAARIKRADTPEISGGKASIE"
     gene            326..991
                     /locus_tag="VN788_13500"
     CDS             326..991
                     /locus_tag="VN788_13500"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HXU21593.1"
                     /translation="MGRLCSARHERDARQRGWLRIAGLDEAGRGSLFGPVVAAVVVLN
                     PRRRIVGLDDSKKLAPDRRSELAPRIREHSIAWAVAEVDASRIDAWNIYQASRQAMVA
                     AISQLSPFPDYLLLDAIELDLPIEQKSLIHGDARSISIAAASILAKVERDRRMQEFHE
                     IYPQYGLSQNKGYGTPDHLEALRQYGPSPLHRFSYAPVREARCWASGAEQTPLQLEPA
                     PGL"
     gene            1183..3930
                     /locus_tag="VN788_13505"
     CDS             1183..3930
                     /locus_tag="VN788_13505"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU21594.1"
                     /translation="MAFNKNKTLESALKFLNQGKIAQAIGEYQQILRHDPKDQATLMT
                     VGDLFARQADMAQAIEYFERLAQVYLHDGFNSKAIAIYKKIAKLAPSELAPLERLADL
                     YVQQGVLSEARPLFLQIAEAHLKANRAPRAVEILHRFLEVEPDNPRVQMRLAELYNVM
                     GQKKEAAQTYLTYAQRLFENGEADEAAKMIERALAVEAGSTSALLLQAKIFAQTNKND
                     AAIAILERLPEADEGGEITNLLIDLEISSQHVSKAADRARKQLARGSAHTALLEKVTD
                     ALLHAGKAAEALPLVCELRGPMVEAGEQDKFLKLLLSSVEKLPDETEPLEMLADFARH
                     TSDPFQLNAALTKLADIYSSNGNYARAEEMLQELVERNKGDERLIARLEKLRTRDESP
                     AEQAPAQIVAEPSNDIFSTREHFGEASLLESPETVAAPKDDEALDEETQRYIAQALTD
                     VDLFSSYGLTQKATNLLENVLKRAPRHTPTLERLLDLYLGASDEQRTAELAFQLEQIH
                     SERGDTVNADRFKELRQRFRKAAGLSEDSPIKPPLATLVSSLSDTHAKTSGSHDPTEA
                     PAFDKAATPTGAAEFEIPLLPMDADSVAPPTAPPQPTELTTTPMSAQEVDLSDEWEAM
                     VQEVEEEAPSEKKESPAPTFELPSEAITDSTEPVRDVQATEPLTAETKTDDQSVEQTN
                     VGTGAGESIADSAEFELELAPEPEPEPRAEKSKTKSTTTEDFLSDLAAEVEDMEMPLQ
                     NPEIAAIPATASPEQPAEAAAITAETLNELTEVFQEFRNELGELGEDGEDLETHYNLG
                     IAYREMGLLDEAIGEFQKVAKAVKEGRPFRYAMNCSTLLAISFMDKGEPRIAALWYQE
                     ALELPGLEQETILALRYDLGVSLDMAGEADAALDNFRQVYAMNIDYRDVSDRIATLQK
                     P"
     gene            4046..4288
                     /locus_tag="VN788_13510"
     CDS             4046..4288
                     /locus_tag="VN788_13510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21595.1"
                     /translation="MKQIARVPILLVWLLFGLALILVPWSDIWETNYFMYQYPGLGLV
                     AENTFLRGVVSGLGFMNVLLSLEAFRRKTSSTANRN"
     gene            4299..4982
                     /locus_tag="VN788_13515"
     CDS             4299..4982
                     /locus_tag="VN788_13515"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HXU21596.1"
                     /translation="MKPIVCYVTDSQSLGVAEPRSDILEKIEAAVRAGVDWVQIREKQ
                     MPARQLLAIAGAAVGLTRHKSSAPGEADRPRSLVLVNDRVDVALAAGAAGVHLGRQSL
                     PVDDVVGWRRRGKTATDFMVGLSCHTIDEVRQAEHAGADYAFFGPIFDTPAKRRFGPP
                     LGVAKLAAACQATRLRVIAIGGVTLETSTECLQAGAAGIAAIRMFQQVREPAELKKVV
                     EGLHALSEQ"
     gene            complement(4992..6050)
                     /locus_tag="VN788_13520"
     CDS             complement(4992..6050)
                     /locus_tag="VN788_13520"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXU21597.1"
                     /translation="MPLEETKLVSTSSASARIIAAGIVIAFCYWASTVLVTLLVSVLA
                     AYFLDPVVTWLEGIRLPRALGSLLVVLLTLALLAVLGWSLIQTVDQFSLDWPEYRQTL
                     RAATQALEKNLETLEAHVSEIEPTERANRGVIAVTESHAVRDALIGRLSSLSVFLLGA
                     SFVPFLVFFMLAGKRQVWHATMQLFAASDRTQVRDTLAEVTQVLRSYLIGTALVGLIL
                     VLANWLFFWGLGLDFPFLTALVSGVCNLVPYLGAIISFIPPLFVGLKQYHTVGPFVGI
                     FVVLTILHILTANFLIPALVGWRIRLNALALTLSILFWGWMWGLMGVILAIPIAAVVK
                     VICEHVESLRPVGRWLSA"
     gene            6218..7582
                     /gene="glmM"
                     /locus_tag="VN788_13525"
     CDS             6218..7582
                     /gene="glmM"
                     /locus_tag="VN788_13525"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HXU21598.1"
                     /translation="MTKQLFGTDGIRGLPGEYPLDDATLDQIGIALGEYVVLHSQAGG
                     TAPRVLLGRDTRESGPHIAARIASGISAAGAMPVSAGVLTTPGVAWLVSRESFAAGVV
                     TSASHNPYHDNGVKLISPLGTKFPDVVEAEFEKSILSRTHRKPLAETSEQADHSFNLK
                     LHEDYLAGLRQTVFPGANLDGMKIVLDCANGAASVLAPKLFRSLGAVILAMHDAPDGR
                     NINAGCGSLHPEGMLRRVIDSGATLGAAFDGDADRAIFATSSGKLVNGDGVMLAAGRY
                     LKATGGLRGNVIVGTTMANLGLERALEKSGLKLARTSVGDRYVLEEMQRIGANFGGEQ
                     SGHILFLDDAPTGDGMLTALKIASLVSIAGTLDELLADLKVFPQRIVNVRVKSKPPLE
                     MLPAVASTLGDAERSLGNAGRVVVRYSGTEPLARVMVEAESEKDVERWTRTLAEALHS
                     AIGC"
BASE COUNT         1934 a         2188 c         2305 g         1567 t
ORIGIN      
        1 gacaaagaac gcattcagcc attcgagggc gtggtgatga gccgtcgagg tgaactgttg
       61 ggcgaaagtt tcacggttcg ccgggtcagt tttggaatcg gcgtggagcg tatctttcca
      121 gtacactcgc cgatgatcag cgccgtggag gtggtgcgcc agggccgcgt ccggcgcgca
      181 aaactcaact atcttcgcca gcgcaagggg aaggcagccc gcatcaaacg cgccgacaca
      241 ccagaaatct ctgggggcaa ggcttcaatc gaataggaat tttagagcca aacgagcaag
      301 tgccgtgtgc taaaattcgc ggccgatggg acggctttgc tcggcaaggc atgagcgaga
      361 tgcgcgccag cgcggctggc tgcgcattgc tggactggac gaagcgggac gcggctcgct
      421 tttcgggccg gtcgtagcgg ccgttgtcgt gctaaatcct agacgccgaa ttgttggcct
      481 ggacgattcc aaaaagttgg cgccggatcg tcgctccgaa ctagccccgc ggatccgtga
      541 acactctatc gcgtgggctg tggcggaagt tgacgccagc cgtatcgacg cctggaacat
      601 ctatcaagcc agccggcaag ctatggtcgc agcaatttcc cagctcagtc cgtttccaga
      661 ttatcttctc ctcgatgcca tcgaacttga cctgccaatc gagcagaagt cacttattca
      721 tggcgacgcg cgatcgattt caattgcggc ggcgtcgatc ctggcgaagg tcgagcgaga
      781 ccgccgaatg caggagtttc acgagatcta tccgcagtac gggctatcgc agaataaagg
      841 ttacggcact ccagatcacc tggaagcctt gcgccaatac ggcccctcac cgttgcatcg
      901 attctcctac gcgccggtgc gtgaagcgcg ctgctgggcc agcggagccg aacaaactcc
      961 gctacagctc gaacccgcgc cggggcttta acgtccacaa gctgagcgtg actagctggc
     1021 taggggaggc agcgcttcgc ctcctgttat cccgttcgtc accacccccg ccaggcgtca
     1081 tggacccccg ttttccgcgc gtgctaacct ttggaccgag ccgctacctc gtcccgaaga
     1141 gaccttctgg ggcaaacgcg gtcaggacac tcgaaagaat tcatggcatt caataagaac
     1201 aagacgcttg agagcgcact gaaattcctt aaccagggga agatcgccca ggccatcggt
     1261 gagtatcaac agatcctgcg gcacgatccc aaggaccagg cgacgttaat gactgtgggc
     1321 gacctcttcg cccgccaggc cgacatggca caggccattg aatatttcga gcgcctcgcg
     1381 caggtctatc tccatgacgg cttcaacagc aaagcaatag cgatttataa gaagatcgcc
     1441 aagctcgctc catctgaatt ggcaccgctc gagcggctgg ccgatctgta tgtgcaacaa
     1501 ggggtgttga gcgaggcgcg acccctcttc ctacagattg ccgaggctca tctaaaggca
     1561 aatcgcgcac ctagagctgt ggagatactg cacaggttcc ttgaggttga accggacaat
     1621 ccgcgggttc agatgcgatt ggccgagctt tataatgtga tgggccaaaa aaaagaggcg
     1681 gcgcagacct accttaccta cgcccagcgg cttttcgaga atggcgaggc agacgaagcg
     1741 gcgaaaatga tcgagcgggc cctggcggtt gaggcgggga gcacaagcgc gttgctcctc
     1801 caggccaaaa ttttcgcgca aaccaacaaa aacgatgcgg cgatcgcgat tctcgagcgt
     1861 ctgcccgaag cggacgaggg cggggagata accaatctac tgatcgacct ggaaatcagt
     1921 tcacaacatg tcagcaaggc agcagatcgc gctcgaaagc agctggcccg tggaagtgct
     1981 catactgctc tacttgaaaa agtcactgac gcattgctcc atgcaggaaa agccgccgaa
     2041 gctttaccgc tggtatgtga gcttcgcggc ccgatggtcg aagcgggcga gcaggataag
     2101 tttttgaagt tgttgttgag ttcggtagag aagcttccgg acgagactga gccgcttgaa
     2161 atgttggccg acttcgcccg gcacaccagc gaccccttcc agctaaatgc cgcgctaacc
     2221 aagctggccg atatttactc cagcaatgga aattacgctc gtgccgaaga aatgttgcaa
     2281 gaattggtcg agcgcaacaa gggcgacgag cgattgatcg cgcgactcga aaagcttcgt
     2341 actcgcgacg aatctccagc agagcaggcg cctgcacaga ttgttgcaga accttcgaac
     2401 gacattttct ctacgcgcga acattttggg gaagcatccc ttttggagtc gcccgaaacg
     2461 gtagccgcgc ccaaggatga cgaagcctta gacgaagaaa cccagcgtta catcgcacaa
     2521 gctctaaccg atgtggatct attttcaagt tacggtctca cgcaaaaagc aacgaacctg
     2581 ctcgaaaatg ttttgaagcg agcgccacgc cacacgccga ccctagaacg tctacttgac
     2641 ctctatttgg gcgccagtga tgaacagcgc accgccgaac tggcttttca gctcgaacaa
     2701 attcatagcg aacgcgggga tacggtcaat gccgacaggt ttaaggagct tcgacaaaga
     2761 ttccggaagg ccgctggact gtcggaggat tcgccgatca agccgcctct ggctacgcta
     2821 gtctcaagcc tctcggacac gcacgccaaa acatccggat cgcatgatcc gaccgaggca
     2881 ccagcgttcg acaaagcggc cacgcccacg ggagccgcgg aattcgaaat ccccctactt
     2941 ccaatggatg cagactcggt cgctccgccg accgcaccgc cacaaccgac agaactgaca
     3001 accactccta tgtccgccca ggaagtagat ctttcggacg aatgggaagc tatggtgcag
     3061 gaagttgaag aggaagcgcc ctcagagaaa aaagaatctc cagcccccac gtttgagctt
     3121 ccttccgaag caatcaccga ttcgacagaa cccgttcggg acgttcaagc aaccgaaccg
     3181 ctcactgcgg agacgaagac agacgatcag tccgtggagc agacgaacgt tgggaccggc
     3241 gcaggggaaa gcatcgctga tagtgccgaa ttcgagctcg aactcgctcc agaaccggag
     3301 ccggagccaa gagcggaaaa gagcaagacg aagtccacga ccacggagga cttccttagt
     3361 gacttggcgg ccgaagttga agatatggag atgcccctcc aaaatccgga gattgccgca
     3421 attccggcca cagcgtcacc tgagcaaccg gcagaagccg ccgcaatcac ggcggaaacg
     3481 ctgaacgaac ttactgaagt tttccaggaa ttccgaaatg agctgggcga acttggtgaa
     3541 gacggagaag atctagaaac ccattacaac ctgggcattg catatcggga gatgggccta
     3601 ctagacgagg cgatcggcga gtttcagaaa gtagctaaag ccgtgaagga ggggaggccc
     3661 ttccggtacg cgatgaactg ctccacactg ctagcgatct ctttcatgga taagggtgag
     3721 ccgaggatcg cggctctatg gtatcaggag gcgctcgaac tgccaggact ggagcaggaa
     3781 acaattctgg cccttcggta tgacttgggc gtttcactcg atatggcagg cgaagcggac
     3841 gccgcgctgg ataacttccg ccaggtttac gcgatgaaca ttgattaccg cgacgtttca
     3901 gaccggatcg caactttgca aaaaccctga tagcggacgg tcgattgcga gcgcagtgca
     3961 aatgcgtcat tcctgctgcg ccgcgcggga tgttacaggc ctgttgccag tccagcaata
     4021 atctcccata atccttgtct ctgcgatgaa gcaaatagca cgtgttccga tactactggt
     4081 ttggctgctc ttcgggctgg cactcatttt ggtgccatgg tcggatattt gggagacgaa
     4141 ttactttatg taccagtatc ccggtttagg actcgttgcg gaaaatacgt tcctgagggg
     4201 agtcgtttcg ggattgggat tcatgaatgt attgctatcg ctagaagctt ttcgccgcaa
     4261 gacttcttcc accgccaacc gcaattgatt atcgcgccgt gaagcccatt gtgtgctatg
     4321 taacggacag ccagtcgctt ggggtagccg agcctcgctc ggacatcctg gagaagatcg
     4381 aagcagctgt gagggcaggc gtcgattggg tacaaatccg cgagaaacaa atgccggcgc
     4441 gacaacttct cgcaattgct ggtgcggccg ttgggctgac acgtcataag tcctcagcac
     4501 caggcgaagc ggatcgcccc agatcgcttg ttttagtaaa tgaccgcgtg gatgtggcac
     4561 ttgcggcggg tgccgcgggg gtacacttag gccgccaatc attgccagtg gacgatgtgg
     4621 tcggctggcg gcggagaggg aagacggcca cggacttcat ggtgggactc tcctgtcaca
     4681 cgatcgacga agtgcgccag gcagagcacg cgggagctga ctacgctttt tttggaccaa
     4741 tttttgacac ccctgcaaag aggcgtttcg gacctccact gggagtcgcg aaactcgccg
     4801 cagcatgcca ggcaactcga cttcgggtca ttgcaattgg cggagtgacg ctggaaacca
     4861 gtacagagtg tcttcaggct ggagcggcgg gcattgccgc aattcggatg ttccaacagg
     4921 tccgggagcc agcggaacta aagaaagttg tcgagggact gcatgctctc agcgagcagt
     4981 aggatggact tctaggcgct cagccagcgg ccaacaggcc gcaagctttc cacgtgttca
     5041 cagatcacct taacgacggc agctatcgga atcgcgagga tgactcccat caaaccccac
     5101 atccaccccc agaatagaat tgatagggtg agcgccagcg cgttcagccg gatgcgccag
     5161 ccgaccagag cgggaatcag gaaattcgcg gttagtatgt gaagaatcgt taagacgacg
     5221 aagattccca caaaagggcc taccgtatgg tattgtttta gacccacaaa gagtggggga
     5281 ataaatgaaa taattgcgcc cagataagga accaggttgc acacgcccga aactagcgcg
     5341 gtaagaaacg gaaagtccag tcccagcccc caaaagaaaa gccagttggc cagcacgagg
     5401 atcaatccga cgagcgccgt cccgattaaa tagcttcgca gcacctgagt gacttccgcg
     5461 agagtatccc gcacctgcgt gcgatcgctc gctgcaaaca attgcattgt cgcatgccag
     5521 acttgccgct tacctgcaag catgaagaag acaagaaagg gaacaaaact ggcacccaat
     5581 agaaataccg atagagagct gaggcggccg atcagcgcgt cgcgaacagc atgtgattcg
     5641 gtgacggcga tcacgcctcg attggcgcgc tcggttggct caatctcgga gacgtgggct
     5701 tccagagttt cgaggttttt ctccagcgcc tgggtggccg ctcggagcgt ctggcgatac
     5761 tctggccaat cgagactgaa ctgatcaact gtctggatca gcgaccatcc aagcactgcc
     5821 agtagagcga gagttaatag aaccacgagg agtgaaccca aagctctcgg caaacgaatg
     5881 ccctcaagcc acgtcaccac aggatcaaga aaataggccg ccagaacgga taccagaagc
     5941 gtgacaagta cggtggaagc ccaataacaa aaggctataa cgatgcccgc agcgatgatc
     6001 cgcgcgctcg ccgaactagt ggagacgagc ttggtctcct caagaggcat ttaagtacca
     6061 taacaatagc acagccatcc gatctcccgg ttccccagcg gcacttcaaa ccgcgctggt
     6121 gacgtttggg gtaacggccg cgagtcgccc ggttcctgcc ttacaacagt gatagaatcg
     6181 cctgccagct cataccggca catggaattc cgcacgcatg actaagcagc ttttcggcac
     6241 ggatgggatc cgtggtctgc ccggcgaata ccccctggat gacgcgacgc ttgaccaaat
     6301 aggaatagca cttggagagt acgttgttct gcactcgcag gcgggtggca cggctccgcg
     6361 ggttctcctc ggccgcgata cgcgcgaatc cggaccgcac attgccgctc ggatcgccag
     6421 tgggatcagc gcggcgggcg cgatgccagt ctcggccggg gtgctaacca ctccgggagt
     6481 agcgtggctg gtgagccgcg agtcatttgc agcaggagtg gtgacatcgg cttctcacaa
     6541 cccgtaccat gacaatggag taaaacttat ctccccctta gggacgaaat tcccggatgt
     6601 agtcgaggca gagttcgaaa agtcgattct ctcgcgcaca caccggaagc cgctcgcgga
     6661 aacgagtgag caggccgatc actccttcaa tctgaaattg cacgaagatt acttggcggg
     6721 tttgcgtcaa acggtgtttc caggggcaaa cttagacggc atgaagatcg tcctagattg
     6781 cgctaatggg gccgcgagtg ttctcgcacc gaaactattt cgatcactcg gagcggttat
     6841 tctagcgatg cacgacgctc ctgatggccg gaatattaat gcaggctgcg gttcgctgca
     6901 tccggagggc atgcttcgga gagtgattga ctccggagcg actctgggtg cggctttcga
     6961 cggcgacgcg gatcgggcga tttttgccac ctcctcggga aagctcgtga atggagacgg
     7021 ggttatgctt gccgccggac gctatttgaa agcaacaggg gggctgaggg gcaacgtaat
     7081 cgttggcacc acaatggcaa atctaggatt ggaacgcgct ttggaaaaat ccggcttgaa
     7141 gttggcccgc acctccgtag gcgacaggta tgtgctggag gagatgcagc gcatcggggc
     7201 aaattttggc ggcgaacaat cgggccacat tctattttta gacgacgcac caacgggcga
     7261 cggaatgctg accgcattga aaattgcttc gctggtttcg atcgcgggca cgctggatga
     7321 actgctagcg gacctaaagg ttttcccgca gagaatcgtt aacgtgcgcg tgaagtcaaa
     7381 gccaccgctt gaaatgttgc ctgctgtcgc ttcgactctt ggtgatgccg aacgttcact
     7441 gggaaacgca gggcgcgtgg ttgtccgcta ttctggaacc gaaccactcg cacgcgtgat
     7501 ggtcgaagcc gagagtgaaa aggatgtgga acggtggaca cgaacactgg ctgaagcttt
     7561 acattccgcc attggatgtt gatttatcga aatccttcca aagaacatag aaagcccggc
     7621 agaaaaatct cataaattgg attattcccg ttgtggcatc ttcctccgtc gttctttgat
     7681 ctgtaacctc ttatgtaact atgagataca tgtatcggag gctcgcgata cgttggcacg
     7741 gtgtttgcag cagcctattc ttgaatcccg gggtgggtcg gcttcgtcgc gccgaggccg
     7801 aacttagggg tagaacaata aggggggcct gggtgggccc ccttttttgc ccggacatac
     7861 cacggatgcc aaatcgctag aaaagacgaa gcattagcgg cacaatcacg ccccaaccat
     7921 gcgagacgat ttcccaccct cgcgtcagtt cgtcggacag cgtcaaagtg atcataatcg
     7981 cgagccagaa gaaa
//
LOCUS       DATNDT010000415         5000 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2112040, whole genome shotgun sequence.
ACCESSION   DATNDT010000415 DATNDT010000000
VERSION     DATNDT010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5000
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2112040"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="VN788_13530"
     CDS             <1..1236
                     /locus_tag="VN788_13530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HXU21599.1"
                     /translation="ELFEEFLTGSEGREVSTTMAFVGMLRKMLRDPEIGKLVVPIVPD
                     EARTFGMESLFRLVGIYNSRGQRYEPVDMNTLLYYKEAKDGQILEEGITEAGSMSSFI
                     AAGSAYATHGINTIPFFIFYSMFGLQRVCDLIWAAADTRCRGFLLGGTAGRTTLAGEG
                     LQHQDGNSHVLALPIPTLRAYDPAFAYELAVIIEDGIDRMYNKGESIFYYITVMNEPY
                     AMPSMPAGARDGILKGMYRYKPAADTKSKLRAQLFGSGAILREVIHAQEILAEKYHVA
                     ADVWSVTSYKALYSDGLEAERWNLLHPTKKPRVPYISRCLADAPGVFVAATDYLKALP
                     NLVSRWMPRRLASLGTDGFGRSESRTSLRNFFEVDARFITLATLHELLLEGKIQASVV
                     QQAIKDLEIDVEKPDPLTT"
     gene            1406..2584
                     /locus_tag="VN788_13535"
     CDS             1406..2584
                     /locus_tag="VN788_13535"
                     /inference="COORDINATES: protein motif:HMM:NF014899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21600.1"
                     /translation="MGKIVAAYATVHAPQLFDRPPSEDPKQLDADIAAMRQIGRELEE
                     TKPDAVIVIGSDHLETFFLSSVPTFAILSGEYSHAEFAGRKYELKIHEGLTEDLLDKL
                     MKASFDLGYSQDAVLGHAFAAVYEWVLEKRPIPVVPIFVNAYLPPLPTAKRCAELGKA
                     IGKIIADRPEKVAIVASGGMSHFPGTWKYPKPDFDFDYWAISQMEKGNHQALINLTNE
                     QLDEVGNTEMLSWMIMFGAIGNQPGELVTYQPTWHHGHAVMRFMPLKGTGPSKEGAEA
                     AKKYEFKGGYEFYKHPSPAAYKLNKVLYDSRFKRELRLRLVHDLPSVAKEYGMTPEEA
                     KVLETLQDDDIEKFRDGQVHPLVEAGAHPLGMWMAVIRMHAELRKLRAAKAEVEKGAA
                     "
     gene            2726..3694
                     /locus_tag="VN788_13540"
     CDS             2726..3694
                     /locus_tag="VN788_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009022219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HXU21601.1"
                     /translation="MKVGVAGQGAFGVKHLEAMANIPGIEVITLTGGNQVATEEVAKK
                     FKIPHWTGDLGESLKQPGLEAMILATPTGMHAKQGEQCMRAGKHVHIEIPIADSLADS
                     ERLVKVQKETGVVCMGGHTRRFNPSHQYVHKKIKAGELKVQQMNVQTYFFRRTNMNAA
                     GKPRSWTDHLLWHHACHTVDLFQYQTGELVSDCYAVQGPMHPELKIAMDMGIVMKVPS
                     GAICTLSLSFNNKGPLGTRFRYICDNGTYIAYYDDLVDGEDKKIDLSHVDVSMNGIEL
                     QDREFFAAIKEKREPNASVAQCLTAMQVLDRLEKILNSVAKATSKA"
     gene            3842..4303
                     /locus_tag="VN788_13545"
     CDS             3842..4303
                     /locus_tag="VN788_13545"
                     /inference="COORDINATES: protein motif:HMM:NF020177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1772 domain-containing protein"
                     /protein_id="HXU21602.1"
                     /translation="MLRNVILFFAALLLGLTAGRAFWVSLGENPFNMHAAVYVEFFQQ
                     LDSRIAIPIAIRGIGGTLLAGISAWFYRRDHTAFLLLLAAFALGATSCLVTIFVNVPI
                     NQQIATWNPAALPADYAALLHRWWNWHHVRLITMLPATCLVLIAMLRPHSS"
     gene            4440..>5000
                     /locus_tag="VN788_13550"
     CDS             4440..>5000
                     /locus_tag="VN788_13550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21603.1"
                     /translation="MKKLGAGNSFGKLGAVVAAGILTVAFAALGPARSTARAANAAAD
                     TGSPLVVGMAFDGHQVANLDTTVKFYETLGYPVKSRTDWKVDKVVNELGGTKGAQSRT
                     AIINMPSSVSDKPFPLILREYRGIKRQDWSKLNSAALGAGHMDVTVLDDCRPVMDKLK
                     AVNMLVVPQMGFPPGPANAPLRFVFVQ"
BASE COUNT         1109 a         1414 c         1412 g         1065 t
ORIGIN      
        1 gaattattcg aggaattttt gaccggaagc gaaggccgcg aagtctcgac caccatggcg
       61 tttgtcggga tgctccgcaa aatgctgcgc gatccggaaa tcggcaagtt ggttgtcccc
      121 attgttccag acgaggcccg cacctttggc atggagtctc tattccggtt ggttggaatc
      181 tataatagcc gcggacagcg ctacgagcct gtcgatatga acaccttgct ctattacaaa
      241 gaggccaagg acgggcaaat cctggaagag gggatcaccg aagccggttc catgtcgtcg
      301 tttatcgccg cgggttccgc gtacgcgact cacggaatca ataccattcc ctttttcatt
      361 ttctattcga tgttcggatt gcagcgcgtt tgcgatttga tctgggccgc ggcggacacg
      421 cgttgccgcg gattcctttt gggaggcaca gccggccgca ccacgctggc aggcgaaggt
      481 cttcagcacc aggacggcaa cagccacgta ctcgcgcttc ccataccgac attgcgcgca
      541 tacgaccccg catttgcgta cgaactcgcc gtgatcattg aagacggcat cgatcgcatg
      601 tacaacaagg gtgaaagtat tttctattat attacggtga tgaatgagcc gtacgcgatg
      661 ccttccatgc cggctggcgc gcgcgatggc atcctgaagg gaatgtatcg ctataagccg
      721 gcagccgaca ccaagtccaa gcttcgcgcg caacttttcg gaagcggcgc gattcttcgc
      781 gaagtgattc acgcgcaaga aattttggcg gagaaatacc acgtcgccgc cgacgtctgg
      841 agcgttacca gctacaaggc gctttacagc gacggcctcg aagccgagcg ctggaatctt
      901 ctgcatccaa ccaagaaacc gcgcgttccc tatatctcgc ggtgtctcgc cgatgctccc
      961 ggagtttttg tggccgcaac ggattatcta aaggcgctgc cgaatctcgt gagccgatgg
     1021 atgccgcggc ggctggcgtc gctgggcacc gatggatttg gccgcagcga aagcagaaca
     1081 tccctgcgga acttcttcga agtggatgca cgcttcatca ccttggctac tcttcatgaa
     1141 ttattgctcg aggggaaaat tcaagcttcg gttgttcaac aggcgatcaa agaccttgaa
     1201 attgacgtcg aaaagcccga cccgctcacc acgtagtcca aactccgcga gccgctcggt
     1261 ttagggtggt ttcgcttgac gaagtcagcc ggtttccgca acaattgcag tatcggctcg
     1321 cctcgagcgt tgcgctctga actcaaggtt tgggcaacga agaatggctg cagcgcgttc
     1381 taatagaatg aagaggtaac taaccatggg aaaaattgtc gcagcatacg ctaccgtgca
     1441 tgccccccag ctttttgacc gcccgccgag cgaagatccc aagcaactcg atgccgacat
     1501 cgccgccatg cgtcagattg gccgcgaact tgaagaaacc aaaccggatg ccgtcatcgt
     1561 gatcggcagc gaccatctcg aaaccttctt cctgagttcc gttccaactt tcgccatttt
     1621 gtccggtgag tattcccacg ccgaatttgc cggccggaag tacgagctga aaatccacga
     1681 gggtctgacg gaagacctgc tcgacaaact catgaaggcc agcttcgacc tgggttactc
     1741 gcaggacgcg gtcctcggac atgcgttcgc agccgtctat gaatgggtac tcgagaagcg
     1801 cccgattccc gtcgtcccga ttttcgtgaa cgcttatctc ccgcccctgc ccaccgccaa
     1861 gcgctgcgcg gagttgggca aggccatcgg caagatcatt gctgaccggc ccgagaaagt
     1921 cgcgattgtt gccagcggcg ggatgtcgca ctttcccggc acctggaaat atccaaagcc
     1981 ggacttcgac ttcgattact gggccatctc gcagatggaa aagggaaatc atcaggcgct
     2041 catcaacctg actaacgagc aattggacga ggtcggcaat accgagatgc tctcctggat
     2101 gatcatgttt ggcgcgatcg gaaaccagcc gggcgaactg gtcacctatc agccgacgtg
     2161 gcatcacggc catgctgtga tgcgctttat gcccctcaag ggaactggcc cgtcgaagga
     2221 aggcgccgag gctgcaaaga aatatgaatt caagggcggc tacgaatttt acaagcaccc
     2281 ctctccggcg gcttacaagt taaacaaggt tctgtacgat agccggttca agcgcgagct
     2341 gcggctccgc ttggtccacg atcttccgtc cgtggcgaag gaatacggga tgacgcccga
     2401 ggaagccaag gttctggaga ccctccagga cgacgacatc gagaaattcc gagatggtca
     2461 agtccatccg ctcgtcgagg cgggcgcgca tcctctcgga atgtggatgg ctgtgattcg
     2521 catgcacgcg gagttgcgca agcttcgtgc cgccaaggca gaagtggaaa aaggcgcggc
     2581 ctagtagggt aactcgcgag cgccttcctc ctgcgtcggt gtccgtcttg tctcaaggcg
     2641 aaaagggaat ccccgcttgg cgctgcgatt tgtgccacgt gctttcaacg atcattgatc
     2701 taaatttcaa agtgagggag aaactatgaa ggtcggtgtc gcgggacaag gtgccttcgg
     2761 cgtgaagcat ttggaggcca tggccaacat tccgggtatc gaagtaatca ctctcacagg
     2821 cggaaatcag gtcgccaccg aggaagtggc caagaaattc aaaattcctc attggacagg
     2881 cgatctcggc gagagcctga aacagcccgg cctggaagca atgatcctcg cgacgcccac
     2941 aggaatgcac gccaagcagg gcgagcagtg catgcgcgcc ggcaagcacg ttcacatcga
     3001 gattcccatc gcggattcgc ttgcggattc cgagcgcctg gtcaaagtgc agaaggaaac
     3061 cggagtcgtc tgtatgggcg gccatacccg ccggttcaat ccaagccacc agtacgtcca
     3121 caaaaaaatt aaggccggtg agctcaaggt ccagcaaatg aacgtgcaga cctacttttt
     3181 ccgtcgaaca aacatgaacg ccgcaggcaa gcccaggagc tggacggacc atctgctgtg
     3241 gcaccacgcc tgccacacag tggacttgtt tcagtatcaa accggcgaat tagtttcaga
     3301 ttgttacgcg gttcagggtc cgatgcatcc tgaattgaaa atcgctatgg acatggggat
     3361 agtaatgaaa gttccttccg gagcgatttg cacgctctcg ctttccttca ataacaaggg
     3421 gccgttgggc acccgcttcc gctacatctg cgataacggc acctacatcg cctactacga
     3481 cgatctggtg gacggagagg acaaaaagat cgatctctcc cacgtagatg tctccatgaa
     3541 tggtattgag ctgcaagacc gcgaattctt tgcggccatc aaggaaaagc gcgagcccaa
     3601 cgccagcgtc gctcagtgcc tcaccgccat gcaggttttg gaccgcctcg aaaaaatcct
     3661 caactcggtg gcaaaggcta cttcaaaggc ttagttgaaa gattccatct ctgggcaaac
     3721 gcacctcctg gcgccggtca ggccagttga tgcgttcccg ccccgaagtc ggttaaactg
     3781 cgctccacaa agtgccttgg tcccggattc ccgtgtaggt cctgtatcag aggaggcagc
     3841 gatgctgcgc aacgtcattc ttttcttcgc agctttgctt cttggtctca cagcgggacg
     3901 agctttttgg gtttcgctcg gcgaaaaccc gtttaatatg cacgctgcag tctacgtcga
     3961 gtttttccaa cagctcgatt cccggatcgc aattccgatc gctatcaggg gcattggcgg
     4021 cacgttgctg gcgggcattt ctgcgtggtt ctaccggcgc gatcacaccg cttttcttct
     4081 gttgctggcc gcgtttgcgt tgggtgcgac ttcgtgtctt gtcacgattt tcgtgaacgt
     4141 cccgatcaat cagcaaatcg ccacgtggaa tcccgcggcg ctgcctgcgg attacgcggc
     4201 actgctccac cgctggtgga actggcacca cgtgcggttg atcacgatgc tcccggccac
     4261 ttgccttgtg ctgatcgcga tgttgcggcc gcactccagt taaacaggtg cgttaggaaa
     4321 ccctcgacgt gaaaaacccg tctctatagg gcaggccgct cggtgtagaa tccagcagaa
     4381 ggaattatgc ctggcaatcg aacgctgtcg tgctgggtcg atatacgggg ggagtgttca
     4441 tgaaaaagtt gggggcaggg aattcgttcg gcaagctcgg tgccgtcgtc gcggctggca
     4501 ttctgactgt cgcctttgca gccctgggtc cggcgcggtc gactgctcgc gccgccaacg
     4561 cagcagcgga cactggatcg ccattggtcg tcggcatggc cttcgacggc caccaggttg
     4621 caaacctcga tactacagtc aagttctacg agacgctcgg ttatccagtg aaaagcagaa
     4681 ccgattggaa agtcgacaaa gtagtgaacg agctgggtgg tacgaagggc gcgcagtcgc
     4741 gaacggcaat cattaacatg ccaagctcgg tgtctgacaa accgtttccg ctgattttgc
     4801 gcgaatatcg tggcattaag cgacaggact ggagcaagct gaattcagcg gccctcggtg
     4861 ccggccacat ggacgtgacg gtgctggacg actgcaggcc cgtaatggat aagctcaagg
     4921 ccgtcaacat gctcgtcgtc cctcagatgg gatttccgcc cgggcccgcg aacgcccccc
     4981 tcagattcgt gttcgtgcag
//
LOCUS       DATNDT010000416        10845 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_388901, whole genome shotgun sequence.
ACCESSION   DATNDT010000416 DATNDT010000000
VERSION     DATNDT010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10845
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388901"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(14..289)
                     /locus_tag="VN788_13555"
     CDS             complement(14..289)
                     /locus_tag="VN788_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21604.1"
                     /translation="MIRHLVQAVFILSFLVFSAPALAAPRSAIALFPNEVAAMRHCPG
                     DTIVWLNTPTRIYHFKGERWYGHTKRGAYVCEKEAEQAGDRASENRQ"
     gene            complement(286..576)
                     /locus_tag="VN788_13560"
     CDS             complement(286..576)
                     /locus_tag="VN788_13560"
                     /inference="COORDINATES: protein motif:HMM:NF024858.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HXU21605.1"
                     /translation="MGAFQDGRGARRHRKLGRQELPIRNFYWTDAASTQRRVAIRLED
                     QFWEALGEIAAAKGMTRSALISELVVEDRDHNFTSMVRVFVLQHFRRKGIER"
     gene            1153..1500
                     /locus_tag="VN788_13565"
     CDS             1153..1500
                     /locus_tag="VN788_13565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21606.1"
                     /translation="MKPELLRAEAKQLRDRARAVADPTAARELHLEVSPPRPYIGEQL
                     AQAKAVEESVFQTFPARLRSDNINAAAPRIRGLPLVGWQLNIAVLPSTSTHFPPLGQT
                     SGPVRYRPSASPP"
     gene            1724..3325
                     /locus_tag="VN788_13570"
     CDS             1724..3325
                     /locus_tag="VN788_13570"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXU21607.1"
                     /translation="MIGNAKTTTTMTAAHDEEGVRAAEYVRMSTDGQQYSIAYQQAAI
                     REHALSHGIKIVRTFADPARSGLSLEDRPALLQLLREVEGGRASYSVILVYDVSRWGR
                     FQDEDESAFYEFICRRAKIEVRYCAEPFESAADLTKTIYKSIKRSMAAEFSRDLSAKV
                     LSAACSKASRGRMPGGRPGFGLRRMVVEDRKNRQSISGHQAETGSKYTVLVLGPKQEE
                     RTVQRIFRLCAIGGLSPSSIARILNREGTKNRDGQRWGGAGVRYILQNEKYTGTLVYN
                     RSSERLGSRRHINPVDQWIRTANALPATVPKHLFDAAQLRIKARHHRHNAASLLALLR
                     RTREKHQKLSVKLIRSETGTDYQPVYRRYFGSLSKAYEIIGYTQRWRNRNLPREFARR
                     QKERDILLHLIEQFSHFQYVVGYDRRARLLVANRSTIAIKLLPFAVTDTRKSPIWCQP
                     AGAARNATITVVGRLNRAETILDYFVLPRSVVRAWIRIGGRFPNRLAKYRAANLSEVV
                     SRLTDKIERDRSAFHRKPITERSAV"
     gene            3370..5010
                     /locus_tag="VN788_13575"
     CDS             3370..5010
                     /locus_tag="VN788_13575"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HXU21608.1"
                     /translation="MNPQSSQGTRAAEYIRVSTDRQIDSEVRQQRVIRDYAAGHGITI
                     VRTFADIGKSGLNLKNRPAFFKLLAEIEKSKYPDFEVILVYDISRWGRFQNTDESAFC
                     EFICTRAGVRVVYCTEPFENAATPIAAVYKTIKRTIAAEYSREQSERISRALNRAANL
                     GHSTGGEIPFGYRRRLVDQKGVHKEFLKKGKLKALRLDHVILAPGPPEDVATVRRIFR
                     LYVDGAYSPRQIARLLNGKGISAGDRPSWTSNRVLYMLSNAKYIGTAIFNRHSGKLRT
                     RMARNPRDKWIVVPNAFPGLISRELFDAAQRRMTERGHTVDQQDLLSQLRRLHDEHGR
                     LSVAILRTPFRDYYQIVHKRFGGLRAASEMIGYDPERAKRDAGRIERRQSHEMRLTGR
                     IISAMSENGCVVKQCRKPKVLVIDQAIVAVRCLAAYAMPPHRQTIWRSNCRKQGAATT
                     VVVGRLDRSGEKILDYLVLPRDVAATPFIINGQFPLRLLPYRRATFADVSATLQGIVR
                     AGRSGGARSRSSRLPSHFRYVRRKVETSAPAKMRRGMR"
     gene            5257..6468
                     /locus_tag="VN788_13580"
     CDS             5257..6468
                     /locus_tag="VN788_13580"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXU21609.1"
                     /translation="MKTATQKLRRRSVPLLLFSAATLVASCNVIPTFGPHLSEVVDES
                     REAERYGFVLVNVNETVLRAITAIPHSSLRTFFPDDRAITPRIGRGDILTITIFETGA
                     GELFAPPSSQQQNYGTNITTLPPITVDPTGNLTLPFVGTIKATGRTPMELQTAIRRRL
                     HGSAIQPQVLVTVTRDVTNVVTVTGTVLHPGRFSLSPASETLTQIIAQAGGSTMLATD
                     TVLQLTRDGRQVGLRLSDLLSFPQQDIHAKPGDYINLIQDRRVFSVYGAVYKSGAYPL
                     PVDDITLAQAISSAGGMNDALADSRGILVFRYEFPEVLKRIPRGRIISAATPTDKSSE
                     PSVPMIYRIDMKTAAGIFYANAFRLRDKDLVFVPSAPTVDWEKYLDLFRLTTSPILSG
                     TTSAVVLDRGF"
     gene            6541..7944
                     /locus_tag="VN788_13585"
     CDS             6541..7944
                     /locus_tag="VN788_13585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HXU21610.1"
                     /translation="MSGGKRQVMIDGFSLAIEEGTGISTYARNLSYALHDAGHQVHVL
                     YGLPIKGVNPLLREVAFFDPQRTGGMKWASTRESMYQALADARDTLTHARAQRVDLSG
                     AVIAAPFRSRLPYFDQLWNARNVFLRADKHFNRTKKFLPVSVPVRPDIAHWTFPLPVR
                     ANRTKNIYTIHDLVPLRLPYTTLDIKRRHYRKLRRICREADHIVTVSENSKKDIINLL
                     GVPEERITNTYQAVAIPEKYVTKPIDVVRREVEGTFNLSYKGYLLFYGAIEPKKNVGR
                     LIEAYLGSKIDTPLVVVGKAAWKSEQELRILNDDVVRYLEHIDSLTVNRSRVQRIDYV
                     PFPLLVSLVRGAKAMLFPSLYEGFGLPALEAMTLGTPVLTSTEGSMPEIAGDAAMLID
                     PYDTAQIADGIRALDSNAELRSELAAKGVSRARLFSTERYRARIEELYDGVFEKKEMR
                     EMNHIDQGDPTWLPSTN"
     gene            7923..8375
                     /locus_tag="VN788_13590"
     CDS             7923..8375
                     /locus_tag="VN788_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21611.1"
                     /translation="MAAVDQLKLLFDRDLKFARWEENAKIESSDPNNVDVRLFTETNE
                     YQFVIENGGTGVPQVTVTVRPRKPRAGQRETRKRHLLTLPQPLNEITWRRVLARIVGL
                     ELVRVQQDKSDASEQRTDNHIRRTGQSLTTFGEPGKAARDMPTGARKS"
     gene            8412..9293
                     /locus_tag="VN788_13595"
     CDS             8412..9293
                     /locus_tag="VN788_13595"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HXU21612.1"
                     /translation="MNDQFAVLERRISALSRQLVRLSTFVETSPGVRSIYVGENTVLT
                     VLADGRKMYVDSRDISISSRLMSRGIWEAANTREIRRLVGPGHCAIDIGANCGYFSVL
                     FSSLVGAEGRVFAFEPNPLAYSLLVRSLEVNGFLQSGIARTFSCALGDQSGTGRLAFK
                     EGNTGGGSLYFTDATILRDNLASIEVEIRRLDEVPIPRDRPTFIKIDAEGAEFLILKG
                     AQRTLEDIDDVVILLEFFPLMIEKHLPIDGYLDFLASFGFSFFRVNGQQLTMVSKAEL
                     LNVPNIYVFLARRDISS"
     gene            9564..10388
                     /locus_tag="VN788_13600"
     CDS             9564..10388
                     /locus_tag="VN788_13600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21613.1"
                     /translation="MKEFSPHDFVANKGPLPFALSDHLFVIRALILRDLRLRNQHRGR
                     LGFLNDLIQPLVVIVLHYYVFIFINRFMPARIPVELYVLGGFTTWFAFRDTSAKNILA
                     IENERGFILIPRIQLAHVLAATAVWETALSIAILYVGLFSSMVLLGSDPLPNVPLSIA
                     AFCIAALLGTGFKLVFDSLGERWPVIKGTKGAVTFLLFLTSGIYFGSTGSPSNQDILN
                     RVVSFNPLFDLLEPQRHALWPGYPVAGISMLYPLTWALGLMFVGLLLRGWVRSRTP"
     gene            10385..>10845
                     /locus_tag="VN788_13605"
     CDS             10385..>10845
                     /locus_tag="VN788_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21614.1"
                     /translation="MIHLESVTMTHALGGTAQKTVLKPTTITLPTDRKLVVLGPDLLA
                     KSAFMRLLAGIEAPPQGRIRLGVRLSPVIKYLALFHPRLSFLENVDFVANLLNVSPGD
                     LALLLEASGYSDLSRSESQQHSEREHRIAAEVALLTLLPFDCYLADEIGHFP"
BASE COUNT         2484 a         2913 c         2962 g         2486 t
ORIGIN      
        1 ccccaagtct gagctactgg cggttttcgg atgcccgatc cccggcctgt tccgcttcct
       61 tctcgcagac ataagcgccg cgcttggtgt gtccgtacca ccgctcgcct ttgaaatggt
      121 agatcctggt cggcgtgttc agccagacga tggtgtcacc ggggcaatgc cgcatggcag
      181 caacctcatt tgggaacagg gcgatcgcgc tgcgcggtgc cgccaaagcc ggtgccgaga
      241 aaacaagaaa ggacaatatg aacaccgcct ggacgagatg tctgatcatc gctcgatccc
      301 cttccttcgg aagtgttgca gcacaaatac tcgcaccatc gaggtgaagt tgtgatcgcg
      361 gtcctcgacc acaagttcgc tgatcaatgc ggaacgtgtc atgcctttcg cggcggcgat
      421 ttctcccaaa gcctcccaga actgatcttc aaggcgaata gcaacacgac gctgcgtaga
      481 cgcggcatcg gtccaataga aatttctgat cggtagctct tggcgtccga gtttccggtg
      541 gcggcgcgct ccgcgcccat cctggaaggc ccccatgcct tcctaataac atcctttgat
      601 gggcggcccc aagcgagtcg gtacgatcgg cgaaatcatc gctagagatt cagatggcgt
      661 gacttggtag tgacgccacc gactgaccgt aaacggcgac gctagcgtcc gaaattagag
      721 aacggcgaca tcgtcggtgt cgacctggat cgcatcctgc agccgggatt ttgtggtgct
      781 atatggccac gcctgatcgc cgaattcaac cagcacgaca tcttacacgg gcctgttcca
      841 tgcgatcgtc aacgtggcgc cgccttaaat tggtttgggc gctaaccggc agtcgcgctt
      901 cggcgtagct ttctttatcc cgtcaacgaa caaaaccgag caacccgcaa tcccgttcgg
      961 tattctcttg tcgtgccgct cgccccggtc ccacgcgccg gcgagcgcat cgacccaaaa
     1021 actgaggcgc ggtctgtttc ctcctggtgc gcccgctttc gccatacggc ggcgactgct
     1081 cgcagacgac attgacgcgg atgcggagca taaacggagc caccagaccg agctaaaggt
     1141 cggctctgct ctttgaagcc agagcttctg cgcgccgagg cgaaacagct tcgggaccgc
     1201 gcacgcgcgg tggcggaccc taccgccgcg cgtgagcttc atctcgaagt gagcccgccg
     1261 cgcccctaca tcggcgagca gttggcgcaa gccaaggcgg ttgaagagag cgtattccag
     1321 actttcccgg ctcggctacg ttctgacaat atcaatgccg cggcgccgcg tattcgcgga
     1381 ttgccgcttg ttggttggca attgaatatt gctgtccttc cgtcgacatc cacacatttt
     1441 ccgccgctcg gacagacctc gggaccggtg cgctatcgac catcggctag ccctccttga
     1501 gagagagaat ctccgggccg aacatgcgta ggggtcagac gcaatcgcaa cgaatattat
     1561 ccaatttcgt ctctaacaag ttggctacgc gctttggatc aactacgtca tcttgtttta
     1621 atgttggcta tggcggcagc cttcccattt caatacgtag caatccgttt ggaatagccg
     1681 gctgatacca aagacgacag ttggttccag cgcacagatt tcgatgatcg gtaacgcaaa
     1741 aaccacgacc acgatgaccg cagcccacga tgaagaaggg gttcgggcag ctgaatatgt
     1801 tcggatgtcg acggatggcc aacaatactc gattgcatat cagcaggcgg caatccgcga
     1861 acatgcgctt agtcacggga tcaagatcgt tcgtaccttt gccgatccgg caagatcggg
     1921 gttgtcgttg gaagatcggc cggcattgct tcaactcctc agggaggtcg aaggtggtcg
     1981 agcaagctat tccgtgattc tggtctacga cgttagtcgt tggggacgat ttcaggacga
     2041 ggacgagagt gcgttctatg aatttatctg caggcgagcg aagatcgagg tgcgctactg
     2101 cgctgagcct ttcgaaagtg ctgcggattt aaccaagacg atctacaaat cgatcaagcg
     2161 gtcaatggcc gcggaattta gtcgcgatct ttcggcaaag gtacttagcg ctgcttgcag
     2221 caaagcatct cgcggtagaa tgcctggggg gcgtccgggc tttggtttga ggcggatggt
     2281 agttgaagac cggaaaaacc gacagtcgat cagtggccac caagccgaga ccgggagcaa
     2341 gtacaccgtc ctcgtgcttg gaccgaaaca ggaagagcgg accgtacagc gcatattccg
     2401 tctgtgcgct atcgggggcc tcagtccgtc aagcatagca agaatcttga atcgcgaggg
     2461 caccaaaaac cgcgatggcc aacgatgggg tggggctggg gtcagatata ttcttcagaa
     2521 cgagaaatat actggaacgc tcgtttataa tcggtcatcg gagaggctag ggtcccggcg
     2581 gcacattaac ccagtcgatc aatggattcg gaccgcaaac gctcttccgg caaccgtacc
     2641 taaacatctg ttcgatgccg ctcaattacg aattaaagcg cgccatcatc gccataacgc
     2701 ggcatcgctg ctcgctctgc ttcgccggac tcgtgaaaaa catcagaagc tttcggtaaa
     2761 gttgatccgc tccgagaccg gaaccgatta tcaacccgtc tatcgacgat attttggaag
     2821 tctgtcaaag gcttatgaaa taatcggtta tacacaaagg tggagaaatc ggaatcttcc
     2881 acgcgaattt gcgcgcagac aaaaggaacg cgacatcttg ctccatttga tcgagcaatt
     2941 ttcgcatttc caatatgtgg tgggatatga ccggagagcg cgattactcg ttgcgaaccg
     3001 gtcaacgata gcgattaaat tattgccatt tgccgtcact gacacacgta aatcgccgat
     3061 atggtgccag ccagcgggtg ccgccagaaa cgcaacgatc acggtcgtgg ggcgactcaa
     3121 tcgtgcggag acgatattgg actattttgt gctgccgagg tccgttgtgc gggcttggat
     3181 tcggattggc ggtcgctttc caaaccgact tgccaaatat cgggccgcga atctatcgga
     3241 ggtagtctcc cgcttgacag ataaaatcga gcgcgatcgc tccgccttcc atcggaagcc
     3301 gatcacggaa agaagcgccg tctagagaaa tatcccttca acggaagcat tacaggttga
     3361 tggtaaaaga tgaaccctca atcttctcag ggaactcgag cggcggagta catccgcgtt
     3421 tccacggacc gtcaaatcga ctcggaagtg cgccaacaga gggtaattcg cgactacgcg
     3481 gcaggtcacg gaataacgat cgtccgaact tttgccgata tcggaaagtc cggtctcaat
     3541 ttgaaaaatc gcccggcctt cttcaagtta ttggccgaaa tcgagaaatc aaagtacccc
     3601 gattttgaag tcattttggt ctacgacatc agcagatggg gtcggtttca aaatactgac
     3661 gagagcgcat tctgcgaatt catatgtaca cgcgctggcg tccgcgtcgt gtattgcacc
     3721 gagccttttg agaatgccgc cacgcctatt gccgccgtct ataagacgat aaagcggacg
     3781 attgctgcgg aatacagccg cgaacaatca gagaggatct ctcgagccct taaccgcgcg
     3841 gccaacctcg gccattccac cgggggtgaa atacctttcg gctaccgacg caggcttgtc
     3901 gaccaaaaag gagttcacaa ggaatttcta aaaaagggca aattgaaagc gctgcgattg
     3961 gatcatgtga tactagctcc cgggccgccg gaagacgtgg ccactgttcg ccgtattttc
     4021 cgtctgtatg tcgatggcgc ctactctcct cgtcagatcg cacggttgct aaacggaaaa
     4081 ggcatcagtg cgggagatcg accatcgtgg acatcaaatc gggtcctcta catgttgtcg
     4141 aacgcgaaat acataggcac tgcgattttc aatcggcatt caggaaaact gcgaaccaga
     4201 atggcgcgca atccccgtga caaatggatc gtcgtcccga acgcttttcc gggtttgatc
     4261 tcaagagaac ttttcgacgc agcgcaacga cgaatgaccg agcgcggcca cacggtcgac
     4321 cagcaggact tactaagcca gcttcgtaga ctacacgacg agcacggccg actatccgtt
     4381 gcgattttga ggacgccatt tagggattat tatcagatag ttcataagag gttcggcggg
     4441 ctccgagcgg cttctgaaat gattggatat gaccccgaac gcgcgaagcg cgacgctggc
     4501 cgaatagaaa ggcggcaatc gcatgaaatg cgactcacag gacgtataat ttccgctatg
     4561 tctgagaacg gatgcgtcgt caaacagtgc cgcaaaccga aggtactcgt aattgatcag
     4621 gcaattgtgg ccgtgaggtg tcttgctgcc tacgctatgc caccgcaccg gcagacgatc
     4681 tggaggtcca attgccggaa gcaaggcgcc gcgacaacgg tggtcgtcgg ccggctcgat
     4741 cgatccggcg agaagattct tgattatttg gttttgcctc gcgatgtagc ggcaacgccg
     4801 ttcattatca acggccaatt tcccctgcga ctattgccat atcgtcgcgc gacgtttgct
     4861 gacgtatccg ccacacttca aggcatcgtc cgggctggcc gaagtggcgg cgcacgatct
     4921 cggtcttcgc gccttccttc acattttcgc tatgtacgtc ggaaggttga aacaagcgcg
     4981 ccagcgaaga tgcggcgcgg tatgcggtga tggaattgaa ggactcgggc tgacacggcc
     5041 cggcgttgct aagtcatgcc aagcggtgtg cgtcggaaaa tcattggttt gccgaaaatg
     5101 aacgtcgacc cgggttggga cgtcagtaag gctcctgtta ccacctcgtc aggcagcgcc
     5161 gcaaaattgc gagcgatttg caggttctgg ccaagtcgcc gggtgtggcc accgtccgca
     5221 tgagtgtcta ctatgtgagt ttatggtggg gagagaatga agacagcgac gcaaaagctg
     5281 cggaggcgat cggttccgct tttgttattt tcggcagcta ctcttgtggc gtcctgcaat
     5341 gtcattccta cgtttggccc gcatctcagc gaagtcgttg atgaatcgcg ggaagccgaa
     5401 cgctacggct tcgtactcgt caacgtgaac gaaaccgtat tacgggccat cacggcgatt
     5461 ccgcatagtt cgttgcgtac atttttcccg gacgaccgcg caattacgcc gcggatcggc
     5521 cggggagata tcctcaccat caccattttt gagactgggg cgggcgagct atttgcgcca
     5581 ccgtcttcgc aacaacagaa ttacgggaca aatatcacca ccctcccgcc catcaccgtc
     5641 gacccgactg ggaatcttac tctaccgttt gtgggaacaa tcaaggcaac cgggcgaaca
     5701 ccgatggaat tgcagacggc cattaggcgc cgcttgcacg gcagcgccat ccagcctcaa
     5761 gtgctcgtca cagttaccag ggacgtaacc aacgtcgtca ccgtaacagg caccgtcctt
     5821 catcccggac ggtttagcct cagcccggca agtgaaactc tgacgcaaat catcgcgcaa
     5881 gccggaggtt caaccatgct tgcaaccgat acggtgttgc aactcacacg cgatggtcgg
     5941 caggttggtc tccgactgtc cgacctacta tcgtttccac agcaggacat ccacgccaaa
     6001 ccaggcgact acataaatct catacaagat cgccgcgttt tctccgttta tggcgcggtc
     6061 tataagtcgg gagcttatcc cttgccggtg gatgacatca ccctcgccca agccataagt
     6121 agcgctggcg ggatgaacga cgccctggca gacagccgcg gcatattagt cttccggtac
     6181 gagtttccgg aggtattgaa gaggattcca cggggtcgga tcatttcggc agctactccg
     6241 actgataagt catcggagcc ttcggtgccg atgatttatc gcatcgatat gaagacagcg
     6301 gcaggcatct tttacgcgaa tgcattccga cttcgcgata aggacttggt atttgttccg
     6361 agtgcaccga cggtagattg ggaaaagtat ctcgatctat tccgtctcac gactagtcct
     6421 attctctcgg gcaccacatc ggcggtggta ttggataggg gtttctaggg tatctgcaga
     6481 gaaagaccgg tttatagcaa tagatggcgc atattccggt gagcggggag ggaaaaatcg
     6541 atgagcgggg gcaagcgcca agtaatgatc gatggtttca gtttagcaat cgaggaggga
     6601 accggcatat cgacttacgc acgaaatctt agctacgcct tgcatgatgc cggtcaccaa
     6661 gttcacgtcc tttatggact tccgataaaa ggggtcaatc cgctgcttcg cgaagttgcc
     6721 tttttcgatc cacaacgtac cggtggcatg aaatgggcat caaccaggga gagcatgtat
     6781 caggctcttg ccgatgctcg tgacacgctg actcatgcac gggcacaacg agtggatttg
     6841 tcaggcgccg taatagctgc cccgtttcgg tcgcgcctcc cgtatttcga ccaactatgg
     6901 aacgctcgca acgtcttcct gcgcgcggac aaacatttca accggactaa aaagtttttg
     6961 cccgtttcag ttcccgtgcg cccggacatc gcgcactgga ctttccctct gcctgtccgt
     7021 gcgaacagga caaaaaacat atacacaatc catgatcttg tgccgctaag gcttccctat
     7081 acgaccttgg acattaagag acggcactac cgaaaactgc gccgcatatg ccgcgaagcg
     7141 gatcacatcg tgaccgtttc cgagaattcg aaaaaagaca tcatcaatct tctcggagtc
     7201 cccgaagagc ggattacaaa tacctatcag gctgtcgcaa tccccgaaaa atatgtcacc
     7261 aagccgatcg atgtcgtgcg ccgcgaggta gaaggtacat tcaacctgtc ttacaagggc
     7321 tatcttttgt tttacggggc gatagagccg aagaagaatg ttggtcgcct gatcgaggca
     7381 tatttgggtt cgaagatcga cacgccgctg gtcgtcgttg ggaaggccgc atggaaatcg
     7441 gaacaggagc tccggatact caatgacgat gtagtgcgat acctcgaaca catcgacagc
     7501 ttgaccgtta accgcagccg cgtgcagcgt atcgactatg tcccatttcc attgcttgtc
     7561 agcctcgtgc gaggtgcgaa ggccatgtta tttccctcgc tatacgaagg attcggacta
     7621 ccggctttgg aagcaatgac tctgggcacg ccggttctca cgtcgactga gggctctatg
     7681 cccgaaatcg cgggcgatgc ggccatgctc atcgatccgt acgacaccgc acagattgcg
     7741 gatggtattc gggcattaga ttcaaatgcc gagctgcgca gcgaactcgc tgcaaaaggc
     7801 gtgtcgcgtg cgcgtttgtt ctcgacggag cgctaccgag cccgaatcga agaattatac
     7861 gacggcgtct ttgaaaagaa ggagatgcga gagatgaatc atattgatca gggtgatccg
     7921 acatggctgc cgtcgaccaa ttgaagcttt tgttcgatcg agacctaaaa tttgcgcgtt
     7981 gggaagagaa cgccaagatc gagtcgtcgg atccgaataa cgttgatgtc cgtctcttta
     8041 cggagacgaa cgagtatcag ttcgtaatcg aaaacggagg caccggcgtt ccgcaggtca
     8101 cggtaacagt cagaccccgc aaaccccgcg ccgggcagcg cgagacccga aaacgccacc
     8161 ttctgaccct tccccagcca ttaaacgaaa tcacttggcg gcgtgtcctg gcaagaattg
     8221 tcggcttgga gttggtgcgc gtgcaacagg acaaatccga cgcgtcggag cagcggaccg
     8281 ataaccatat tcggcggact ggccagtccc tcacaacatt tggggagcct ggaaaggccg
     8341 cgagagatat gccaactggc gctcggaaat catagctgtt cagccgattc ccactatcca
     8401 agggagagcg cgtgaacgat caatttgcag ttctcgaacg gagaatatct gccctatccc
     8461 gacagttggt aaggctatcg acctttgtcg aaacctcgcc gggggtaagg tcgatatatg
     8521 tcggcgaaaa tacagtgctg acagttcttg ccgatggccg aaaaatgtat gtcgactctc
     8581 gggacataag catcagttcg cgcctcatgt cgcgcggcat ctgggaggcg gccaacacgc
     8641 gcgagataag acggctcgtc ggccctggtc attgcgcgat cgatatcggg gcgaactgtg
     8701 ggtatttcag cgtcttattt tctagtctcg ttggagcgga ggggagagta tttgcgtttg
     8761 aaccaaatcc gctcgcctat agtcttttgg tccgatccct ggaggtcaac ggcttcctcc
     8821 agagcggcat tgctaggaca ttttcttgcg cactgggcga tcagtcgggt accggacggc
     8881 tcgcgttcaa ggaaggtaac accggtggtg gatcgctata ttttacggat gcgacgattc
     8941 tccgagacaa tctagcgtcg atcgaggtcg aaattcgcag attggacgaa gttccaatcc
     9001 caagagatcg accaaccttc ataaaaattg atgcggaggg cgcagagttt ctgatcttga
     9061 agggagccca gcgaactttg gaggatatcg acgatgttgt aatcctcctg gagttctttc
     9121 cgctgatgat tgaaaaacac ttacctatcg acgggtattt ggacttcctc gctagtttcg
     9181 gattctcctt ctttagggtg aacggccagc aattaacgat ggtttcgaaa gccgagcttc
     9241 taaatgtgcc caacatatat gtcttcttgg cgcgccgaga tataagctcc tgaaactgtt
     9301 gtagctttgg gcgcgagcgc tgcgctttga caaaagtcct ttccgggagt agttctcgca
     9361 cgtcgtcgtt atgaaaacga catggtagag tgggagcgag ccgttatggc tgctggcggt
     9421 ggcgagtttg ccaaccccga aagcgcagag gcaggatctg cttattgcgt atggccattt
     9481 cacaaggcca gatttctatc tcccaatcgc gaaagattgt ccattcagcc gcagcggcaa
     9541 ttcgtcggat caggtccgcc gcgatgaaag aattctcgcc acatgacttc gtcgcgaaca
     9601 aagggccgct cccgtttgcg ctatcggatc atctttttgt cattagagcc cttatcctcc
     9661 gggacctccg tttaagaaat caacacagag gccgcctggg attcctaaat gatctcattc
     9721 agccgctagt tgtcatcgtc cttcattact acgtctttat atttatcaac cggtttatgc
     9781 ccgcgcggat tccagttgag ctgtatgtac tcgggggctt tacaacctgg ttcgctttca
     9841 gagatacgtc ggcgaagaat atcctcgcga tcgagaatga gcgtggattc atcttaattc
     9901 ctcggattca gttggctcac gttcttgctg ccacggccgt ttgggagacc gccctatcca
     9961 tcgccattct ctacgtagga ttgttcagct cgatggtgct attagggagc gatcccttac
    10021 ccaatgtgcc gctgagcatc gcagcatttt gcatcgcagc gttattggga accggattca
    10081 agttggtctt tgatagcctg ggtgagcgtt ggccggtcat taaaggaacg aaaggggccg
    10141 tcacttttct gctttttttg acgtcaggca tttactttgg ttcaacaggc tctcctagca
    10201 accaggacat cctgaaccga gtcgtttctt tcaatccgct tttcgacttg ctcgaaccac
    10261 aacgacacgc tctttggccc ggttatcccg tcgccggaat ctcaatgctt tatccgctta
    10321 cgtgggcgct aggcttgatg tttgttgggc ttttgctccg gggttgggtc aggtctagga
    10381 cgccatgatt catctcgaat cggtaacgat gacacacgct ctcgggggca ctgctcaaaa
    10441 gacggttcta aagcccacaa cgatcacctt gccaaccgac cgaaaactcg ttgtgcttgg
    10501 ccccgatttg ttagccaaat ctgcgttcat gcggttattg gcggggattg aagcgccacc
    10561 gcaaggccga atcagattag gtgtccgctt gtctccggtg attaaatatt tggctttatt
    10621 tcatcctcgt ctcagttttc tggaaaatgt cgattttgtt gcgaacttac tcaatgtaag
    10681 tccgggcgat cttgccttgt tgctggaagc gtcgggttat tcagatttat cgcggagtga
    10741 gtcgcagcaa catagcgagc gcgagcaccg cattgctgct gaggttgcgc tgctcacgct
    10801 gcttcccttc gactgctatt tggcggatga aatcggccat ttccc
//
LOCUS       DATNDT010000417         6221 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3664781, whole genome shotgun sequence.
ACCESSION   DATNDT010000417 DATNDT010000000
VERSION     DATNDT010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3664781"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            162..2015
                     /locus_tag="VN788_13610"
     CDS             162..2015
                     /locus_tag="VN788_13610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HXU21615.1"
                     /translation="MSTTLVRPPQRKRELIDRAVIRFCGDSGDGMQITGSQFTNTVAL
                     YGNDLATLPDFPAEIRAPAGTLPGVSGFQVHFSSSDVYTPGDSVDALIAMNPAALKMN
                     IADLKPNGILIVNLNDFGETDLRKAQMTANPLEDHSLDAYRLFSVELTKLTRAALKDL
                     GLDNKTVDRCKNFFALGMCYWLYNRSLDNSLRWIEEKFKSKPSLVQANRTAMQAGYSY
                     CEATEAFQISYEIPPAKLAPGLYRNISGNTALAMGFVAASQKAGIPLFLGSYPITPAS
                     DILHELSGYKDFGVITFQAEDEIAAITSAIGAAYAGALGITSTSGPGMALKTEALGLA
                     VAVELPLVICDIQRGGPSTGLPTKTEQADLLQALFGRNSEAPIPVLAPATPGDCFWVA
                     LEASRIAIKYMVPVIILSDGYLANGAEPWKIPDLSQLPDIAVKFEKNPEGFHPYRRNP
                     ETLARPWAIPGTPGLEHRIGGLEKQDVTGNVNYEPLNHERMVRLRAEKVAAIVQDVPD
                     AVPEGDPSGELLIVAWGSTHGPITAALKAQRAKGRRIGHVHLHHLNPMPKNLGEVLGR
                     YDKVLVPEMNMGQLIMLLRAKFLVDAHGYNKIQGKPFKQSEIEDKIEELLD"
     gene            2140..3168
                     /locus_tag="VN788_13615"
     CDS             2140..3168
                     /locus_tag="VN788_13615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013629173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HXU21616.1"
                     /translation="MTTATLPVYTKKDFQTDQEIRWCPGCGDYAILSAVQSVFPELGI
                     PREKFVVISGIGCSSRFPYYMNTFGFHTIHGRAPAVATGLKIAQPDLEVWVATGDGDS
                     LSIGGNHTIHMLRRNVGLKVLMFNNKIYGLTKGQYSPTSEIGKKTKSTPFGTADRPFN
                     PISLAIGAEATFVARSVDVFQQHLKDTLKKAAAHQGSAYIEILQNCNIFNDHAWETLT
                     ERDARSEHVVQLEHGKPLIFGKNRDKGIRLEGFDLEVVPLGNGVTEKDLLVHDEHHPR
                     PAYAFLLAHMEQRTGFPTPIGVLRSWNDVPRYEDVISKQIRDVQERRGKGDLEKLLRA
                     GDTWEVRP"
     gene            3318..4547
                     /locus_tag="VN788_13620"
     CDS             3318..4547
                     /locus_tag="VN788_13620"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU21617.1"
                     /translation="MKRLLVFVALVFFASIAGATHLAAQDLAITNVRIIVGNGNVIEN
                     GSIVARGGKIVSVAAGAPNASGMQTIDAHGMTAVPGFIDGHHHLMGRNPEQYLKTQAV
                     QDMQNFLDAGYTTVLAGRGPVPGILQLKQKIDSGELKGPRIIPSEGVSLRNITPDEAR
                     AQIRKLAAMRIHFIGEEGLSNDPKPEEIEVLKALVDESKKIGGVYVMVHAVSIPAMLA
                     SVDAGAPLLVHTPQFGWVSDADAKKVADAGVKELSTIGFGVPVFGVFANDNVPRFRDG
                     KPWPSGIIGGEGQGREAGYKAVNARTLWDAGVVYGYGTDTDYLPKAGLSHELRSLNLM
                     FSPQDLIKLMGPNSAAFIQMSDQLGTLEVGKFADIVIVDGNPLNGYWNLLNTRLVIKG
                     GVVVSDQRKSPAPAEDH"
     gene            complement(5050..5706)
                     /locus_tag="VN788_13625"
     CDS             complement(5050..5706)
                     /locus_tag="VN788_13625"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009179908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU21618.1"
                     /translation="MPLIKNGERPTTTRETQCKKPTGFFGRLTLRRMNNSHSELTDWG
                     LTHTTIESRFTVLDVGCGGGRTISKLAAMAAQGKVHGIDYSEESVAASRRYNAQAIRD
                     GRVEIHLADVGKLPFPDNTFDLVTGVETHFWWPEIAAGLREIRRVLKPGGTLILIAEV
                     YKGADTIMSRLCEKQAPISGIKMLTPDEHRALLALAGFADLRIDALAAKGWITAQGRK
                     "
BASE COUNT         1331 a         1986 c         1612 g         1292 t
ORIGIN      
        1 tttgcctctt ccacagcgcg acggtgtggg caccggctcc agtttgtgct acgcttttgc
       61 gggacgagaa gttcgcggcc gcgcattagt ttggaaaacg gctccggcat ctcgctttcc
      121 tttacgctca tctaaaagag cagggataca ggcaggcact catgtctaca actttggttc
      181 gtcctccgca gcgcaagcgc gaactgattg accgcgcggt tatccgcttc tgcggcgatt
      241 ccggcgacgg catgcagatc accggaagcc agttcaccaa tacggtcgcg ctctacggca
      301 acgatctcgc cacgcttccg gactttcccg cggaaattcg tgctcccgcc ggcactttgc
      361 ccggcgtcag cggatttcag gtgcatttct cgtcgagcga tgtctacacc ccgggcgaca
      421 gcgtcgatgc actcatcgcc atgaaccccg ccgcgctgaa aatgaatatc gccgacctca
      481 agccaaacgg catcctgatc gtcaacttga acgattttgg cgaaaccgat ctgcgcaagg
      541 cgcagatgac cgcgaaccct ctcgaagatc attcgctcga cgcctatcgc ctgttttcgg
      601 tcgagctgac gaagctcact cgcgccgccc tgaaagattt gggcctcgac aacaaaactg
      661 tcgatcgctg caaaaatttc ttcgcgctcg gcatgtgtta ttggctttac aaccgctcac
      721 tcgataattc gctccgctgg atcgaggaaa agttcaagag caaaccctcg ctggtgcagg
      781 ccaaccgtac cgcgatgcag gcgggttaca gctattgcga agccaccgaa gccttccaaa
      841 tcagttacga aattccgccc gcgaaactcg cgcctggcct ttaccgcaat atcagcggaa
      901 ataccgcact cgccatgggt ttcgtcgccg cttcgcagaa agcgggaatc ccgctttttc
      961 tcggctccta tcccatcacg cccgcctcgg acattcttca cgaactttcc ggctacaaag
     1021 atttcggcgt gatcaccttt caagccgagg acgaaattgc cgccatcact tcggcgatcg
     1081 gcgctgcgta cgccggcgcg ctcggcatca cttcaacctc cggccccggc atggcgctga
     1141 aaacggaagc gctgggcctc gccgtcgccg tcgaactccc cctcgtcatc tgcgatatcc
     1201 agcgcggcgg accctccacg ggccttccga cgaaaaccga gcaggccgat ttactgcaag
     1261 ctctctttgg acgaaactcc gaagcgccca ttcccgtcct cgcgcccgca acaccaggcg
     1321 actgcttctg ggtcgctctc gaagccagcc gcatcgccat caagtacatg gtgcccgtca
     1381 tcattctttc cgacggctac ctggccaacg gcgcggagcc ttggaaaatt cccgatctct
     1441 ctcagttgcc ggatatcgcc gtgaagttcg aaaaaaatcc ggaaggcttc catccgtatc
     1501 gccgtaatcc tgaaaccctc gcgcgcccat gggccattcc cggcactcct ggactcgagc
     1561 accgcatcgg cggcctcgaa aagcaagacg tcaccggcaa cgtgaattac gaaccgctaa
     1621 accacgagcg catggtccgc ctccgcgccg aaaaagtcgc ggcaatcgtt caggacgtgc
     1681 ccgacgccgt gcccgaaggc gatccttccg gcgaattgct gatcgtcgcc tggggctcca
     1741 cgcacggtcc gatcaccgcc gcgctgaaag cgcagcgcgc caaaggccgc cgaatcggcc
     1801 acgttcactt gcaccatttg aaccccatgc cgaaaaatct cggcgaagtt ctcggccgct
     1861 acgacaaagt tcttgtcccc gaaatgaaca tgggccagct catcatgctg ctccgcgcaa
     1921 aatttctcgt ggacgcgcac ggctacaaca aaattcaggg caagcccttc aagcaatccg
     1981 agatcgaaga caaaatcgag gagttgctcg actaatgccc gctctcatca aacaattgtc
     2041 atcccgagcg aagtcacgcg cgacttttcc tcccgcgctt tccgcgggcg cgcgacgcag
     2101 cgagggatcc ctcttaggca ttcccacgga gaagtctaaa tgaccacggc cactttaccc
     2161 gtctacacaa aaaaagattt tcagaccgac caggaaatcc gctggtgccc aggctgcggc
     2221 gactacgcca ttctttccgc cgtgcaatcg gttttccccg agctcggcat tccgcgtgaa
     2281 aagtttgtcg tcatttccgg aattggctgt tccagccgct tcccgtatta catgaacacc
     2341 ttcggcttcc acaccattca cggtcgcgcg cccgcagttg ccaccggact gaaaatcgcc
     2401 caacctgatc tcgaagtctg ggtcgccact ggcgacggcg attccctttc catcggcggc
     2461 aatcacacca tccacatgct gcgccgcaat gtcggcctca aagttttgat gttcaataat
     2521 aaaatttacg gcctcaccaa gggccagtat tcgcccactt ccgaaatcgg caagaaaacg
     2581 aaatccactc ccttcggcac cgcagaccgc cctttcaatc ccatctcgct cgccatcggc
     2641 gccgaagcca ccttcgtcgc gcgctccgtc gacgtctttc agcagcatct gaaagacacg
     2701 ctgaaaaaag cggccgctca tcagggttcg gcctacatcg agattctgca gaattgcaat
     2761 atcttcaacg atcacgcctg ggaaacactc accgaaaggg acgcgcgcag cgagcacgtg
     2821 gtccagcttg agcatggcaa gccgctcatc ttcggcaaaa atcgcgacaa gggcatccgc
     2881 ctggaaggtt tcgatctcga agtcgtaccg ctcggcaacg gcgtcaccga aaaagatctt
     2941 ctcgtgcatg acgaacatca ccctcgaccc gcctacgcat tcctgctcgc gcacatggag
     3001 cagcgcaccg gcttccctac tcccatcgga gtgctccgct cctggaacga cgttccgcga
     3061 tacgaggacg tcatctccaa gcagattcgc gatgtgcagg aacgtcgcgg caaaggcgac
     3121 ctcgaaaaac tccttcgcgc cggcgatacc tgggaagtcc gcccttagcg atccgcgtcc
     3181 ggtccgcgcc gcgtactcgc tgcccagcac gcaccagaaa atcgaaaccc tcccgcctta
     3241 gaaaccgtcc catgtctgca taattgtcgt catccaggcg gtcaataaca gcaatccgga
     3301 atcgagggct tgaaccaatg aagagacttc ttgtttttgt ggcgctggtt ttctttgcga
     3361 gcattgctgg cgccacgcat ctcgcggcgc aggacttggc tatcaccaac gtccgcatca
     3421 tcgtgggcaa tggtaacgtc attgagaatg gctccatcgt tgctcgcggc ggaaagattg
     3481 tctccgttgc cgctggcgcg ccaaacgctt ccggcatgca gaccattgat gcccacggca
     3541 tgaccgcggt tccgggcttc atcgatggcc accatcacct catggggcgc aatcctgaac
     3601 aatatttgaa gacccaagcc gttcaggata tgcagaattt cctcgatgcg ggttacacca
     3661 ccgtgctcgc gggccgtggc ccggtccctg gaatattgca gctcaagcaa aaaatcgatt
     3721 ccggcgaact caaaggccct cgcatcatcc catccgaagg cgtcagttta agaaacatta
     3781 ccccggacga ggcccgtgcc caaatccgca aactcgccgc gatgagaatc catttcatcg
     3841 gcgaagaagg actcagcaac gatccgaagc ccgaagagat tgaagttctc aaagccctcg
     3901 tggacgaatc gaaaaaaatt ggcggtgtct atgtaatggt ccacgccgtc agcattcctg
     3961 ccatgctcgc ctccgttgac gccggtgcgc ctctgctggt tcatacaccg caatttggct
     4021 gggtgagcga cgcggatgcc aaaaaagttg ccgacgccgg ggtgaaagag ttgtcaacga
     4081 tcggcttcgg ggtgcccgtc ttcggcgtgt ttgcgaatga taatgtgcct cgtttccgcg
     4141 atggaaagcc gtggccttcc ggaattatcg gcggcgaagg ccagggacgc gaagcgggtt
     4201 acaaagccgt gaacgcgcgt acgctctggg atgccggcgt ggtctatggt tacggcacgg
     4261 acaccgacta tctgccaaaa gcgggcctgt cccacgaatt gcgatcgctg aacctcatgt
     4321 tctcgccgca agatctcatc aagctgatgg gaccgaactc ggcggcgttc atccagatga
     4381 gcgatcagct cggtacgctc gaggtaggca aattcgccga catcgtcatc gtggatggca
     4441 atccgctgaa cggctactgg aatttgctga ataccaggct ggtcatcaag ggcggcgtgg
     4501 tcgtatccga tcagcgcaaa tccccagccc ccgccgaaga tcactgatgg atacgcggtt
     4561 ggcggaagcc tccgcatggt ggaaagccag cgttcacgcg gaggaaatca accggatgcg
     4621 tgaagatggt gtttggtgga agcccccgca ttcatgcggg ggaaacaacg cctatccaag
     4681 aatgtgctta gccccggtgc agctcctgct tgtgtagcgc caccctttca ggggcggcat
     4741 cttttggaat cccgcgtttg cctcttgccc ttctgaaaag actgctagcc gttttttccc
     4801 tgcgtccgat ccatttggag gctcccgaca gacggtcaat cgcagcttcc atgtcagaga
     4861 caccggacgg cattcctact ctctcgcccg cataatctaa gacggacttg gacagtccat
     4921 caaaggagat caaggataca tacgtcggat attagtcatc taattgacat cttccttctg
     4981 gtactctact tctccgatca gcgcgtcaat caggagtttg gaaagttgct ctcaaccttt
     5041 ttttgtcttt tatttcctcc cctgagccgt gatccagccc ttcgcggcga gcgcatcgat
     5101 tcgaaggtct gcaaatccgg ccaaggccag cagagcgcga tgctcgtcag gagtgagcat
     5161 ctttatcccg ctgatcggcg cctgcttttc gcataagcga gacatgattg tgtccgcacc
     5221 cttgtacact tccgcgatca ggatcagagt gccgcctggt ttcagcacac gccggatttc
     5281 gcgcaatccg gcggcgattt ccggccacca gaagtgcgtc tcgactccag tgaccagatc
     5341 aaacgtgttg tcagggaagg gaagcttccc gacatccgcc agatgaatct ccacgcggcc
     5401 gtcccggatt gcctgcgcgt tgtaacgccg ggacgcggca acactctcct ccgagtagtc
     5461 gattccgtgg accttccctt gcgccgccat cgccgccaat ttgctgatgg tgcgcccgcc
     5521 accgcagccc acatccagca cggtaaatcg cgattcgatg gtggtgtgcg tcaggcccca
     5581 gtcggtcaac tccgagtggc tgttgttcat gcgccgcaga gtcaagcgcc cgaagaatcc
     5641 cgtcggcttc ttgcattgcg tttcccgcgt cgtcgtcggc ctctcgccgt tcttgattag
     5701 gggcatggga acctcgcgca aacgatacgc acgccgctag cgctccccgg cagaaggaca
     5761 gggcttccct gtcccgagcg aagccgaggg aagctctacc gcaaaaacga cagcgcgaag
     5821 cgccttccag cttgccctga gtgctgttgc gaagggtgcg ctgcttttct cgcagcatgt
     5881 actcgcattc ccgccttaga agccccacct acaaccaccg tctccttcag ccgagcgggc
     5941 ccgctcgcca cttctcactt ttttgcctcg cgccgtctcc aacatccagc ctctacactc
     6001 tagcctccag atcgtgttga tgccacgcct gccttccttc gtcagggtaa agagggatgg
     6061 caccagattt gttgtgtccg agagagggag aagcgtacga taaacctaga agtcgaaatt
     6121 gggattctag cttttccgtg ctcggcggca tggtgaaagg aacaataacg tggcccgatc
     6181 cgaacctgaa aaatggatcg ccctgtttgc gaacaaattt c
//
LOCUS       DATNDT010000418         7303 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3836127, whole genome shotgun sequence.
ACCESSION   DATNDT010000418 DATNDT010000000
VERSION     DATNDT010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7303
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3836127"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..868
                     /locus_tag="VN788_13630"
     CDS             71..868
                     /locus_tag="VN788_13630"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HXU21619.1"
                     /translation="MNQFLEVAIEAAREAGAMLRPEFDRPKKISYKGEVDIVTESDRR
                     SEALIVAHLRRHFPDHAIIAEEGSNSAASGSRYCWHVDPLDGTTNFAHGYPCFAVSIG
                     LTEDGEPIAGAVFNPIADELFSAARGEGAYLNEKPIRVSSVEKLAHSLVATGFPTHQR
                     KRSANMEFYWEFTLRSHGVRRDGSAALDLCSVACGRFDAFWEFRLNSWDTAAGMLLVQ
                     EAGGTVTDLGGQPYRPGGFVLLASNGRVHDEMREIAAGIAERAEQNA"
     gene            883..1974
                     /gene="queA"
                     /locus_tag="VN788_13635"
     CDS             883..1974
                     /gene="queA"
                     /locus_tag="VN788_13635"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HXU21620.1"
                     /translation="MNVSELDFDLPPERIAQYPLAERDASRMLLLDRVSGEWEDEQFR
                     DLPKLLRGDELVIVNNVKVVPARLFGRRAGIHSEMPGRNNSARNEFLQAPIEVLLVRQ
                     IGADMWETLVRPGRKVPAGERIIFGDGELEALVEGRGEYGLRVLRFTSSSGFHEALGR
                     LGHIPLPPYIKRADEPLDRERYQTVYAKRGCAIAAPTAGLHFTPEIIAELRGRGIQIA
                     EITLEVGLGTFEPVRTEKIEDHTIHAEVYEIPVETASALGRAKREKRPVLAVGTTVVR
                     ALEDAAGKSTGSEEPVAAGKGEAAILLYPGKPFRVVNQLLTNFHLPRSSLLALVAAFA
                     GRESILRAYRHAVESGYRFYSYGDSMLIR"
     gene            2075..2818
                     /locus_tag="VN788_13640"
     CDS             2075..2818
                     /locus_tag="VN788_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013581880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HXU21621.1"
                     /translation="MRYLILSDIHSNLTALDAALDAAKGRWEEAVCLGDVVGYGPDPN
                     EVIDRLRELKVFAIRGNHDKAVAGLADAEEFNPVARAAALWTRERLSPENLDYLEKLP
                     TGPVSRDGFSMIHGAFHDEDEYVFSPALAVDGLLSTPTDISFFGHTHIQGGFALRGED
                     IQVLHLTPAGKNSTSAFQPESGTKYLLNPGSIGQPRDGDPRAGFAIADIEHGSIEFWR
                     VGYDIETVQQRMRLAKLPEALVLRLSLGR"
     gene            2832..3329
                     /locus_tag="VN788_13645"
     CDS             2832..3329
                     /locus_tag="VN788_13645"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HXU21622.1"
                     /translation="MAEPGEKDSSEERSQFPAAFAVGVVIMVILAAGLVLLTRVTRRH
                     PPGAGEKPPFGPTEQAYASQIHFQPGEMSQSSNLLNQEFTYVAGTVTNGGKRTVRALN
                     VNFEFHDPFNQVVLRDAQILIDPAGQPLGPGQSRPFQVTLGAHLPSEWNRAYPLIRIT
                     GMILE"
     gene            3366..4865
                     /locus_tag="VN788_13650"
     CDS             3366..4865
                     /locus_tag="VN788_13650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21623.1"
                     /translation="MNDWAELSDALDGITASGTAEIREDGEWLAELAAPRCELRLEGK
                     SPLVHLWSDKRNLTRRVLRVRERSQDRVVLEVQRFGRARPSRLEVVRAQSPRPAKRVA
                     REQFCAGFTRILADCFPDATIEPLTASPDLENSFSGVYARGYMYEGSRAWAMMAVAPG
                     ESTAAMEGILAFGVLWLDWLRGRAQVRAIEGLRLFLPEGAGNCVRERCAALSSNTGVE
                     IFEFRVEDGRIFKMDCADTGNLISLLVPRGEAESTRSAARDAIRRIHALALHIPPAGS
                     EIGLRLVPGTAEVSLGFRGLKFGSWSSKDGISFGLGDSHEKLTSSNELALDELMRTLD
                     LWRSPLAKDTRHSLYRAAPERWLEALVLDDPTRLDAQLDPRHLYSQVPALTAGDRGVL
                     DLLGVTRRGRLVVIELKASEDIQLPIQAVDYWLRVCRHQRDDDFHRHGYFAGIELDPK
                     PPLIWIAAPALRFHSATDVILKYVSPEIRITRIGLNENWRRSLKVVFRQ"
     gene            complement(4975..5484)
                     /locus_tag="VN788_13655"
     CDS             complement(4975..5484)
                     /locus_tag="VN788_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HXU21624.1"
                     /translation="MQAPVLVLNATFEPINVTAVRRALILMLKGVAQAEETSHTEVHS
                     TSKAIQVPSVIRLLSYRHIPQQSRALSRKNILLRDRNTCQFCGTPFPASELTLDHVMP
                     RSRGGRSSWENLVASCYSCNNRKGDRTPEEAGLKLQRRPRPFTLHTSRQLMRLIGHRD
                     EKWRKYLFY"
     gene            complement(5643..6458)
                     /locus_tag="VN788_13660"
     CDS             complement(5643..6458)
                     /locus_tag="VN788_13660"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HXU21625.1"
                     /translation="MILKGALGALASVVFASPCRICGEALTNASRIPICEKCLDGFEK
                     IVEPMCAGCGRPFATAGAGAVGSPLCRLCRVGFYGFDRARSFAIYDEALAEAVLLLKY
                     QELARLGDWFAERLAGVVSRMPAEWRADLVVPIPLHADRRRERGYNQAELIARPLADR
                     LGIPFNSRILVRTKPRPAQLVLSRTEHWKSVRGAYAIRPAANVDNVRVLLVDDVLTTG
                     ATLDAGARVLKKAGAATVLGLTVGRVRSGLPPPSSVLKRKRPNGKPEGPVEHL"
     gene            complement(6588..6941)
                     /locus_tag="VN788_13665"
     CDS             complement(6588..6941)
                     /locus_tag="VN788_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HXU21626.1"
                     /translation="MDKKRVDYYKKKLAARREELLRTIARTQQEGREADEDPTVDLAD
                     KAANSYTKEFLFGQTHNERSLLQLVDDALSRIRENSFGDCVSCHQELQQKRLEAVPWT
                     RYCISCQEKKENGQL"
     gene            complement(7119..>7303)
                     /locus_tag="VN788_13670"
     CDS             complement(7119..>7303)
                     /locus_tag="VN788_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane glutamate
                     endopeptidase"
                     /protein_id="HXU21627.1"
                     /translation="DSGVVLKGHLMNASFHGPAWLNGGTVGPEGSVLVAAVLALAAIG
                     IHYIFPKRPTPEDQPA"
BASE COUNT         1492 a         2071 c         2197 g         1543 t
ORIGIN      
        1 gtggtccggt atcgcaagga gtacttcacg cccgcggtga cggaatgatg ggcttgcatg
       61 cgaggcgcgc gtgaatcaat ttcttgaagt tgccatcgag gcggctcgcg aagcgggcgc
      121 aatgctgcgg ccggaattcg atcgtcccaa gaagatttcg tacaaggggg aagtcgatat
      181 cgttaccgaa tccgatcgac gctcggaggc gctgattgtc gcgcacctcc gccggcactt
      241 tcccgatcac gcgattatcg cggaggaagg cagcaacagc gctgcgtccg gatcgagata
      301 ttgctggcac gtcgatcctc tcgatggcac cacgaatttc gcgcacgggt acccatgctt
      361 tgccgtctcg attgggctga cggaggatgg tgagcccatc gcgggcgcgg ttttcaatcc
      421 catcgcggac gagcttttta gtgcggcgcg cggcgagggc gcatatttga atgagaagcc
      481 tattcgcgtc tcgtcggttg aaaagttggc gcatagcctc gttgccacgg gttttcccac
      541 tcatcagcgc aagcgaagcg cgaacatgga gttctactgg gaatttacgc tgcgctccca
      601 cggggtacgg cgcgatggct ccgcggcgct cgacctctgc tctgtggcgt gcggccgctt
      661 cgacgcattc tgggaattca ggctgaactc gtgggatacg gcagcgggaa tgctgctggt
      721 ccaagaggcg ggcgggaccg ttaccgatct cggcgggcaa ccatatcgtc cgggcggttt
      781 cgtcttactt gcgtcgaatg gccgtgtcca tgacgagatg cgagaaattg ccgctggaat
      841 cgccgagcgc gcagagcaga atgcctaacc ggtagccgcg cgatgaacgt aagcgaactc
      901 gattttgatt tgccgcctga gcgaattgcg cagtatccgc tggcagaacg cgacgcttca
      961 cggatgctcc ttctcgaccg agtttcgggt gagtgggaag atgagcaatt ccgcgacttg
     1021 ccaaagctgc ttcgcggcga cgaactcgtt atcgtcaata atgtgaaggt ggtccccgca
     1081 cgactcttcg ggcggcgagc cggcattcat tcggaaatgc ctggccgcaa caattcagcc
     1141 cgcaacgaat tcctgcaagc gcccatcgag gtgctgctgg tccggcaaat cggcgcggac
     1201 atgtgggaga cgctggtgcg gcccggaagg aaagttccag cgggcgagcg aattatattc
     1261 ggtgatggcg agcttgaggc tttggtggaa gggcgcggcg aatacgggtt gcgcgtgctg
     1321 cgcttcacat cgagtagcgg ctttcacgag gcgcttgggc ggcttgggca tatcccgctg
     1381 ccgccataca taaagagggc ggacgagccg ctcgatcgag agcgctatca aaccgtttac
     1441 gcaaaacggg gctgcgccat cgcggcccca accgcaggct tgcattttac gccagagatc
     1501 atcgcagaac tgcgcgggcg cggcatccag atcgcggaga ttacactcga ggtaggactc
     1561 ggaaccttcg aacctgtacg aaccgaaaaa atcgaggacc atacaataca cgccgaagtc
     1621 tacgagattc ccgtcgaaac ggcgtccgcg ctcggccggg cgaagcggga aaagcggccg
     1681 gtgcttgccg tcggaaccac ggtggtacgc gcgctggaag atgccgccgg gaagagtacg
     1741 ggaagtgaag agccggtagc ggctggaaag ggcgaggcgg cgatacttct ttatcccggg
     1801 aagccgtttc gcgtggtgaa tcaattgctc accaattttc acttgccgcg atcgagtcta
     1861 ctggcgctgg tggcggcctt cgccggccgc gagagtattc ttcgcgcgta tcgacacgcg
     1921 gtagagtctg ggtaccggtt ctacagctac ggggattcca tgctgatccg ctaacgaaat
     1981 tggagatttg ggttcttact aggagtaaag cgatagctct cttgcaagcg gcatgcgttc
     2041 gtctatgatg gttcggtttg ctggaaggcg ggcgatgcgc tacctgatcc taagcgatat
     2101 ccactctaac ttgacggccc ttgacgccgc gctcgatgcc gcgaaaggcc gctgggaaga
     2161 ggctgtgtgc ctgggggacg tagttggata tggtcccgat ccgaacgagg taattgacag
     2221 gcttcgcgaa ctgaaggtgt tcgctatacg cggcaatcac gataaagcag tcgcgggcct
     2281 cgcggatgcg gaggaattca acccagtcgc gcgtgccgcc gctctttgga cgcgcgagag
     2341 gctttctccc gagaacctgg actatctgga aaaactcccg accggcccgg tatcacggga
     2401 tggattttcc atgattcacg gagcctttca cgacgaggac gaatacgtct tctcccccgc
     2461 gctggcagtg gatggcctgc tcagcacccc taccgatatt tcattttttg gtcacacgca
     2521 cattcagggt ggattcgctc ttcgaggaga ggacatacag gtgctgcatc tcacacctgc
     2581 gggaaagaat tcaacttctg cttttcagcc ggagtccggt acgaaatacc ttctcaatcc
     2641 cggctcaatc ggccagccgc gagacggcga tccgcgagcc ggtttcgcga ttgccgatat
     2701 cgagcacggg tcgattgagt tctggcgcgt cgggtatgat attgaaacgg tgcagcaacg
     2761 aatgaggttg gctaagctgc cagaggcatt ggtcctccga ttatcacttg gccgctaaga
     2821 aaaataagaa aatggcagag cccggagaga aggacagtag cgaggagaga agccagtttc
     2881 ccgccgcgtt tgccgtcggg gtggtaatca tggttatttt ggctgctgga ctggtgctgc
     2941 tcacccgcgt gacgcgacgc catcctccgg gcgcgggcga aaagcctccg ttcggcccca
     3001 cagaacaagc ctacgcatca cagatacatt ttcagcccgg cgaaatgtcg cagtcttcga
     3061 atctgctgaa ccaggagttc acctacgtcg cgggaacggt taccaatggc gggaagcgca
     3121 cggtgcgagc tctgaatgtg aacttcgagt ttcacgatcc gttcaatcaa gtggttctgc
     3181 gggatgccca gattttgatc gatccggcgg gccaaccgct cggcccggga caaagccgcc
     3241 cttttcaggt cacgctgggc gcccaccttc cctccgaatg gaatcgggcg tatcctctga
     3301 tccgcatcac cggtatgatt ctcgaatagc atcgtaattg ttttctaaac gagtggcggt
     3361 tgtctatgaa cgattgggcc gaattgagcg atgccttgga cggcattacc gcttctggga
     3421 ctgcggagat tcgggaagac ggcgaatggc tggcggaact ggccgcgccg cgctgcgaat
     3481 tacgcctgga aggcaagagt cccctcgtac acctgtggtc cgataaacga aacttgaccc
     3541 gacgtgtgtt gcgagtccgc gaacgatcgc aggatcgcgt ggtgctcgaa gtgcagcgat
     3601 ttggccgcgc aaggcccagc cggcttgagg ttgtgcgcgc gcagtcgcct cgtcccgcca
     3661 agcgggtggc tcgggaacaa ttttgcgctg gattcacacg gatactggcc gactgtttcc
     3721 ctgacgcaac gattgaaccg ctcaccgcat cacccgacct cgagaattct ttttccggag
     3781 tatacgcgcg gggatacatg tacgagggat cccgtgcctg ggccatgatg gcggtcgccc
     3841 ccggcgaaag tactgccgcg atggaaggca ttctggcatt tggagttttg tggcttgact
     3901 ggttgcgagg ccgcgcacag gtgcgcgcga tcgagggctt gcgacttttc cttcccgagg
     3961 gtgcaggcaa ctgcgtgcga gagcgttgcg ccgcgctttc atcgaatacg ggcgtcgaaa
     4021 tctttgagtt tcgagtggag gacggcagga tattcaagat ggactgcgcc gacaccggga
     4081 acctaataag tttgctggtg cctcgcggcg aggctgaatc aacgcgcagc gcggcacgtg
     4141 atgccattcg acggattcac gcccttgcgc tgcacatccc tccggcgggc agcgagatcg
     4201 gcctgcgtct tgtgcccgga acagccgagg tttcgctcgg ttttcgagga ctcaaattcg
     4261 ggagttggtc atcgaaggat gggatttcgt ttggcctggg agattcacac gagaaactta
     4321 cgtcctccaa cgaactggcg ctcgacgaac tgatgcggac tctcgatttg tggcgcagcc
     4381 cactcgcaaa ggatacacgg cacagtttgt atcgtgcggc gcccgagcgt tggctggaag
     4441 ccttagtgct ggatgatccc acgaggctgg acgctcaact cgatccgcgt catctttatt
     4501 ctcaggtacc cgccctgacg gcgggagacc gaggtgtgct cgatttactt ggggtaacgc
     4561 ggcgaggacg tctcgttgta atcgaattga aggcctctga ggacattcaa ttgccaatcc
     4621 aagcggtgga ctactggttg cgtgtttgcc ggcatcaacg cgacgacgat tttcatcgcc
     4681 acggttattt cgctgggatc gaactcgatc ccaagccgcc gcttatttgg atcgccgctc
     4741 cggccttgcg attccattct gcgacggacg tcatactgaa atatgtttcg ccggaaattc
     4801 gaatcacgcg tattggactg aacgagaatt ggcgccgcag tctgaaggta gtcttccgcc
     4861 aatagtcaga aaaaagggaa gcggtccggc ttaataccgg ctccgcctcc cggggtgtgt
     4921 tctaggagtt actcaattag acgcaatcac ccggctaggg ttgcccggat tagttcagta
     4981 gaagagatat ttgcgccatt tctcatcccg atgtccaatc aggcgcatta attggcgcga
     5041 agtgtgcagt gtgaaaggac gcggtcgccg ctggagcttt aatcccgcct cttcaggagt
     5101 gcggtcaccc ttgcgattgt tgcaggaata gcaggacgca acgaggttct cccaggagga
     5161 tcgtccgccg cgtgatcgag gcatcacgtg gtcgagagtc agttccgacg cgggaaaagg
     5221 cgtgccgcaa aactggcagg tgttccgatc ccgcaacaaa atattcttcc gcgaaagggc
     5281 ccggctctgt tgcggaatgt ggcggtagct gagcagacgg atcaccgatg gcacttggat
     5341 ggccttggaa gttgaatgga cttccgtatg gctcgtttcc tccgcttgtg ctactccctt
     5401 cagcattagg attagcgcac ggcgaacggc cgtcacgttg attggttcaa atgtggcgtt
     5461 caaaaccagc accggagctt gcatgaccga aggtctgccc gcccgagcgg gctgcggctt
     5521 cgcggacaca gaatcaaccg gcgggctgcc cagccctaaa atgggccggg gccgtaggcc
     5581 gaatttgtgg cggctgtcca tcgcattacc gtcacttgaa cgcttcacac tgttccttga
     5641 tactatagat gctcgaccgg cccctctggt ttgccatttg gtctcttccg ctttaaaacg
     5701 gatgagggcg gaggcaggcc cgaacggacg cgacccaccg tcaatcccag aacggttgcg
     5761 gcccccgctt tcttcagcac tcgagcgcct gcatccaggg tggctccggt agtcaataca
     5821 tcatctacca gcaggacgcg cacattgtca acattcgctg ctggcctgat agcgtacgcg
     5881 ccacgcaccg atttccagtg ttccgtccga gagagcacca gttgggccgg ccgcggtttc
     5941 gtgcggacca ggattcgcga attaaaaggg atgcccagcc ggtcggccag gggccgcgca
     6001 atcaactccg cctggttgta gccgcgctcg cggcgacggt ccgcatgcag tgggataggc
     6061 acgacgagat cggcgcgcca ctctgcaggc atgcgtgaga ccaccccagc taaccgttcc
     6121 gcgaaccaat cgcccaaccg cgccagttct tgatatttga gcaagagaac ggcttcggca
     6181 agggcctcgt cgtaaatcgc gaagctgcgc gcgcgatcaa agccgtaaaa accaacgcgg
     6241 cacaaccggc aaagtgggct tccgacagcc ccggcccccg ccgtcgcgaa cgggcgtccg
     6301 caaccggcgc acatcggctc gacaattttt tcaaagccgt ccaggcattt ttcgcagatc
     6361 gggatgcggc tggcgttggt cagggcttcg ccgcaaatgc ggcacgggct ggcaaacacc
     6421 accgatgcaa gcgcgccgag ggcgcctttc aggatcacgg atacccgtcc agaatcgcga
     6481 actatggccg gacattactc caagtcctga atgaaggaat agagatgatc gtctctagag
     6541 agttgaaaat tgcgaagtct gccagacggg caagaaccgc aaaactctta taactgcccg
     6601 ttctctttct tttcttggca gctaatgcag tagcgggtcc aaggcacggc ttccaaacgt
     6661 ttctgctgta gctcctgatg gcacgaaacg cagtcgccga acgaattctc ccgaattcgc
     6721 gacagcgcgt cgtctaccag ttgcagcaaa gatcgttcgt tgtgtgtttg gccgaacaga
     6781 aactccttgg tataggaatt cgcggccttg tcggcaagat ccacggtcgg atcctcgtcg
     6841 gcctcgcgtc cttcttgctg cgtgcgcgcg atcgtccgga gcagctcctc gcggcgcgcc
     6901 gcgagtttct ttttgtaata atccactcgc ttcttatcca tggtctgcac tctactgtgt
     6961 tccagatggc tcagccaaat cgggcgattc taaacgtccg aaccgccctt gtcaaacaag
     7021 catcgtcgca aatccttaaa tccttcatcc cttgtagtca ttcatgcagc ttccagtaga
     7081 tgcgtcgtgc gcccaaaagg attccacgct gcgttctctt acgcgggttg atcttccggt
     7141 gtcggccgtt ttggaaaaat gtagtgaatg cctatcgcgg ccagcgccaa gaccgcggcc
     7201 accaaaacgc ttccctccgg tccaaccgtt ccgccattca gccacgccgg cccgtgaaac
     7261 gatgcattca tcagatgccc tttcagcacc acgccgctat ccg
//
LOCUS       DATNDT010000419        11784 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_798565, whole genome shotgun sequence.
ACCESSION   DATNDT010000419 DATNDT010000000
VERSION     DATNDT010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11784
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_798565"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="VN788_13675"
     CDS             <1..111
                     /locus_tag="VN788_13675"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004299836.1"
                     /note="catalyzes the formation of
                     (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-
                     carboxamido)succinate from
                     5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate and
                     L-aspartate in purine biosynthesis; SAICAR synthase;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HXU21628.1"
                     /translation="DWNKQPPGPALPADVVAGTRERYREAYQRITGHALD"
     gene            162..380
                     /locus_tag="VN788_13680"
     CDS             162..380
                     /locus_tag="VN788_13680"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU21629.1"
                     /translation="MKEQLEALVGTMVERGILLEEALTEFEKKFIKRVLEESKGNQCR
                     AAKILGIHRNTLSRKITEYHLDSNGRRR"
     gene            510..1481
                     /locus_tag="VN788_13685"
     CDS             510..1481
                     /locus_tag="VN788_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HXU21630.1"
                     /translation="MIAKGLASTKHPLLVHIIPMRRCNLACTYCNEFDDFSKPVPLDE
                     MFRRIDRLGAMGTSVITISGGEPLLHPELDAIIARIRQNGIIAGLITNGYLLVAERIE
                     RLNRAGLEYLQISIDNVQPDDVSKKSLKVLDQKLKLLAEYAEFQVNINSVLGSGVRNP
                     EDALTVAHRALELGFTSTVGILHDGNGQLQPLGAREQEIFEEIMSLGKRSFTRVNRFQ
                     HNIAAGRENDWRCRAGSRYLYVCEDGLVHYCSQQRGYPGIALAEYTEEQRQREYLTKK
                     GCAPRCTVSCVHQISVVDGWRSAQKLKPNPRAPSEPFVQLTGPAAQD"
     gene            1577..3163
                     /locus_tag="VN788_13690"
     CDS             1577..3163
                     /locus_tag="VN788_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HXU21631.1"
                     /translation="MATTAEYSGSVPEVNPWLIAATVMLATFMEVLDTSVANVSLPHI
                     AGSLSASVDESTWILTSYLVSNAIVLPLSGWLSGVFGRKRFFIACIVIFTVSSLLCGL
                     APSLSALVVCRVLQGAGGGGMQPTSQAILIDSFPREKQGMAMAIYGMGVVIAPTVGPT
                     LGGWITDNYSWRWIFFLNVPIGVISVLMISFLIAGDRSRKELRGKNQGIKVDFLGLGF
                     LGLGLGLLQVVLDKGERDDWFGSPFILWATIIAVAALIALVIRELTTDHPVVELRLFR
                     DRNFAISTFMMYVLGVVLYGTTLLLPILMQTLMGYTAMQSGLVLLPGGVVLLGTLPFV
                     GWLLGKVEHRWVVMAGLCITALGLYQLSHLTLNAGVSKAIYAWIISRAGTAFLFVPIN
                     VMAFYFVPKGNMDNASGLINLARNVGASTGISFVTTMLDRRAQLHQNILAHNMQGGNR
                     LYQDALNHLAHLFTAHGSDAAHAIVQAHAVLYGQLQRQATMLSFVDNFRTMAIICICV
                     IPLMFVMKGRRPQAEQLRIH"
     gene            complement(3201..3821)
                     /locus_tag="VN788_13695"
     CDS             complement(3201..3821)
                     /locus_tag="VN788_13695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019490918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HXU21632.1"
                     /translation="MMSDRDKPAPAAVPINDLLSHRWSPRAFEARLVEPEKLRTLFEA
                     ARWAASSYNAQPWSFIVATKDDPDNFKKVLDSFVEFNQGWAKHAPVIGLSVAAHKMPH
                     DGAQNRHAFHDVGQAATSLALQATALGLQAHQMAGILPDKARQIFGIPEDHEAVAGIA
                     LGYPGDPSSLPDPLRQREMGVRDRKPASSFVFTGAWGKVAPVVSKS"
     gene            complement(3890..5701)
                     /locus_tag="VN788_13700"
     CDS             complement(3890..5701)
                     /locus_tag="VN788_13700"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXU21633.1"
                     /translation="MTKLFALLALLVAASMPAAYAAQAPVPPGEKDLTLQAMHDEMER
                     ARTRLSLPGVEKPFYIEYRLLDLDIRSVTASFGTLLSSSTSRSRFMSVDVRIGDYHLD
                     SSNFMSEDGFQGFLGSTGQVGIDRDYKSLRQDLWLATDQAYKAAATQMSLKQAFLRSL
                     TKPPEIDDFSQATPLVKIDPRIEPDWTSRNWEDEARAASSGLKDFPQLYGTRVNYSLI
                     YVTSYLMTSEGTIIRESRHLAAIEAALDTEADDGMPLNDYYAVYSSEPSGLPAPIAVS
                     KGLGQTAVELLTLRAGALVPDYTGPVLFDPSAAGSALAQVLEPSLSGARPPLSMTQDL
                     NAFLERFGGRSEWTGRVGTRVLPASVSLVDDPTATEFKGQPLLGAYDVDDEGVKGERV
                     PIVENGILRNLLMSRRPGPDFSASNGHARSALLMDTKPLSSNLFFQSSDTLKPDDLRK
                     KFIDACREDGHEWCLEVKRMANPVVSSVHQQDFTDFVGAIAGGIASGERMPLTVSRVY
                     VADGHEEPVRGGVIEGLTLRSFRNILGIGDDAAVYTYMQNPADNLAGTALGAFGSAQG
                     GIPSTVIAPSLLLDDVEIRGFHGEPRRLPLVPAPPLK"
     gene            complement(5698..7413)
                     /locus_tag="VN788_13705"
     CDS             complement(5698..7413)
                     /locus_tag="VN788_13705"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HXU21634.1"
                     /translation="MRHRSFTIGVALLAAAWGCWAAGRPTPATPPVLTAMQEELDRSM
                     AALGKADPSAYFISYTVSDREYAQVSGSNGALLSSSEDRGRWLEVQTRVGTAQLDDTH
                     KLGDRPPSWNSPGTTVALDDDIPVLRREIWRETDRQYRAASQALIKVKTSQQVQVQTA
                     EGAAPDFSHEEPHTSVGPRVEIHVNRKPWEERVRRYTAEFSQSPAVLNSIATFTAQAT
                     NQYQVNTEGTKLAFGLIHYRLELFVQSKAPDGMDIQRYANFDWLDPKDAPDDKTVSNS
                     IHRLIEETEALDKAPLVEPYAGPALLTGRAAAVFFHEVFGHRAEGFRQKDITEGQTFT
                     SKIGQHILPDFISIKDDPTEATLGGQMLLGNYPFDDEGVPSEDVSLVDHGVLKTFLMS
                     RSPLMDIPHSNGHGRRQLGYVPIARQGNLIVTSSNGVPFGELRRKLIALVKQQNLPYG
                     LLIDDIAGGFTFTGRGQPQAFQVTPLIVYKVFADGRPDQLVRGVDIVGTPLVSLTKIV
                     GTENKQEIFNGYCGAESGSVPVSAVAPGILISEMEFAKKQTSTDRPPILPPPAHDPEA
                     KGPQQ"
     gene            7551..8585
                     /locus_tag="VN788_13710"
     CDS             7551..8585
                     /locus_tag="VN788_13710"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXU21635.1"
                     /translation="MRALVAKRYGAPEVLEAQEVPDPQPKAGEALIRVKTIGVNFADL
                     LQRMGIYPGTPKPPFIPGLELAGVVERVVEGPRSPGGDPLKPGDAVVALTNFNAYAQW
                     AAVPLNRVYRLLPGMGFEDGAAIPGNYVTAYHALFSMGNLQPGDRVLIHGAAGGVGIA
                     AVQLARARGLTIFGTAGPSKQEYLRKIGVDHAIDYEKTDFVQTVRKFAPDGIEMVMDP
                     IGGKSFKRSYECLGAGGRLVVYGMSAAAGPDGKRSLIRGLTALLQIPRFNPLELMRRG
                     VSVMGINLGAMEARPAVLRGELEEIFRLYAAGKIKPVIGKIFPLDQAAAAHRYIHDRK
                     NIGKVILSVK"
     gene            8723..9109
                     /locus_tag="VN788_13715"
     CDS             8723..9109
                     /locus_tag="VN788_13715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21636.1"
                     /translation="MRSIGGIVGLVVIALIAGLTYKYYFAQGQSAGVTTPRQTIDVVG
                     VKNDLVAIGQAERLYQAEHGSYASLDDLVSSGAMSMKKTGRDGYTYEAETSADGFRIT
                     ANCASDTAPGCTSYAIDQTMEVQTAP"
     gene            complement(9147..10343)
                     /locus_tag="VN788_13720"
     CDS             complement(9147..10343)
                     /locus_tag="VN788_13720"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0044571 - [2Fe-2S] cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IscS subfamily cysteine desulfurase"
                     /protein_id="HXU21637.1"
                     /translation="MALKLPVYLDNHATTAVDPRVLDAMLPYFTEKFGNAASRSHEFG
                     WKAEDAVEKARGQIARLIHASPREIIFTSGATESNNLAIKGVAEAYGGKGNHIISQAT
                     EHKAVLDTCKRLEISGSEITYLPVHSDGLIDLENLRGAITPKTILISIMYANNEIGVI
                     QPIEAIGKIAKEKKILFHVDAAQAVGKIPIDIQKDGIDLLSISAHKIYGPKGVGVLYV
                     RRKDPRVNLSSMIDGGGHERGMRSGTLNVPGIVGLGQACELCQKEMAEESERSRRLRD
                     KLKDAITSSLDGTSINGSMIHRLPNNLNVSFTGVEGDALLMGINDVAVSSGSACTSAT
                     LEPSYVLRALGVPEDLAHSSIRFGIGRFNTDEEIEYAAGRVIETVKRLRELSPFYEAA
                     KPNASR"
     gene            complement(10630..>11784)
                     /locus_tag="VN788_13725"
     CDS             complement(10630..>11784)
                     /locus_tag="VN788_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21638.1"
                     /translation="TLMEYIYQFYYQTYYQLKQYPKAIEYADKLIALDKANLALRVQA
                     LQARVQLFGLAFDAKAPDAHDQLVKERDAALLGVKLFPDLKKMPNSKVTDEQVKEGTA
                     FLEAAAGAADLQLKDYPASIEAFKAGLAINPKDAVTDYRLGLAYLATTPPQPLDGFWY
                     LARAIDLKVPDDAKVKDYLRSRILAYEQPGCDSQVDPQLNELLQLAANSPDRPATYTI
                     PSHDDLTKISQASTIITVIADLSGGGDKAKTTWLAICGAEFPEVVGKIIDVQKSEGYV
                     DFMVFTSANSDEMQAATMANMDVKVWTAPKPAGTPAPNANAANLPPVAPQPDVVRLSK
                     DDPIRFSGMLVGYDPSPFILHWDQVKVDPSVIPEKAGGAKKPMPRRTPQH"
BASE COUNT         2545 a         3261 c         3376 g         2602 t
ORIGIN      
        1 gactggaaca agcagccgcc ggggccggcc cttccggcgg atgtagtcgc cggcacccgc
       61 gagaggtatc gagaagctta tcagcgcatc accggccacg cactcgactg aggtccaggg
      121 aacacactca tcctccgagc gttgatgtgg agcgtgcaca agtgaaagag caattggaag
      181 cgctggtcgg cacgatggtg gagcgcggga ttcttctcga ggaagcgctc acggagttcg
      241 aaaagaagtt catcaagcgc gttttagaag aatccaaagg aaaccaatgc cgcgcggcca
      301 agattcttgg aatccaccga aatacgctga gccgaaagat taccgaatac cacctcgata
      361 gcaacgggcg ccgccggtag caaccaccgt gtggcataat tgagttcggg agacgactta
      421 acctgcgttt gccccggcag cctgtgacac tcaagaagaa aatacggaag aagttgctcg
      481 cggctcatcg caaggtgcgc gagttccgca tgattgcgaa gggcctcgcc tccacaaagc
      541 accctctatt agtccacatt attccgatgc ggcggtgcaa tctggcatgc acatactgca
      601 atgaattcga tgacttctcg aagcctgtgc cgctcgatga gatgttccgc cgaatcgacc
      661 gtcttggggc gatgggaact tccgtgatca cgataagcgg aggagagccg ctgcttcatc
      721 cggagctcga cgcgatcatc gcgcgcatcc ggcaaaacgg aatcatcgcg ggccttatca
      781 ccaatggata tcttttagtg gcggagcgca tcgagcggct gaatcgcgcg ggccttgaat
      841 atttgcagat cagtattgat aacgtgcagc cggacgacgt atccaaaaag agcctgaagg
      901 tgctggacca gaagctcaaa ttgctggccg aatacgctga atttcaggtg aacatcaatt
      961 cggtgctggg tagcggcgtt cgcaatccgg aagacgcgct gacggtggcg catcgcgcgc
     1021 tagagttggg attcacatca accgtgggaa tcctgcatga cggcaacggc cagctacaac
     1081 cgctgggcgc gcgggagcag gaaattttcg aggagatcat gtctttggga aaacggtctt
     1141 tcacgcgcgt gaaccgtttt cagcacaata tcgcggcggg gcgagagaac gactggcgct
     1201 gccgcgcggg gtcgcgttac ctctacgttt gcgaggatgg cttggtccac tattgctcgc
     1261 agcagcgcgg gtaccccggg atcgcgctcg ccgaatacac cgaggaacaa cggcagcgcg
     1321 aatacttgac gaaaaaaggc tgcgctccgc gttgcaccgt ttcctgcgtt caccagatat
     1381 cagtcgtgga tggatggcgc agcgcgcaga agctgaagcc gaatccgcgc gctccttccg
     1441 aaccattcgt tcaacttacc ggaccagccg cacaagacta gtgcgcgccc cgatctttcc
     1501 attggcaacg ccaagacagc atgctttggc gaatctgatc gttactccgc ctctgctcgg
     1561 acgaatccaa tagccgatgg ctaccactgc cgaatattca gggtcggttc ccgaggtaaa
     1621 cccgtggctt atcgccgcta cggtgatgct ggcaacgttc atggaagttt tggacaccag
     1681 cgttgccaat gtgtcgctgc cccatatcgc gggcagtctt tcggccagcg ttgacgagag
     1741 cacctggata ctcacgtcct acttagtatc caacgcgata gttttgccgc tttcaggatg
     1801 gctatccgga gtttttggcc gcaaacgatt cttcatcgcc tgcatcgtaa tcttcactgt
     1861 tagctctctt ctttgtgggc tggcgccttc gttgagcgcg ctggtagttt gcagggtgct
     1921 tcagggcgcg ggaggcggcg ggatgcagcc tacatcgcag gcaatcctga tcgatagttt
     1981 cccgcgcgaa aagcagggaa tggccatggc aatctacggc atgggagtgg tgatagcgcc
     2041 cacggtggga ccgacactcg gcggatggat taccgacaac tattcctggc gatggatttt
     2101 ctttttgaac gtgcccatcg gagttatttc cgttctgatg atctcgttcc tgattgccgg
     2161 cgaccgctct cggaaagagc tgcgaggaaa aaatcaggga attaaggtcg attttctggg
     2221 attgggattt ctgggcttag gtctagggct gctgcaggtg gtactggata agggcgagcg
     2281 agatgactgg ttcggatcgc catttatttt gtgggcgacg ataattgctg tcgcagcgct
     2341 cattgcgctg gtgatccgcg aattaaccac tgatcacccc gtcgtggagc tccgtctctt
     2401 tcgagaccgg aatttcgcga tttccacatt catgatgtac gtgctcggag tcgtcttgta
     2461 cggcacaact ctattgcttc ctatcctgat gcagacgctg atgggttaca cggcgatgca
     2521 gagcggactc gtattattgc cgggaggagt agtgctgctg ggcaccctgc cgttcgtggg
     2581 atggctgctg ggcaaagtcg aacatcgctg ggtggttatg gccggattat gtatcaccgc
     2641 gctcggcctg taccaacttt cgcatctcac tctcaacgca ggcgtgagca aggcgatcta
     2701 cgcctggata atatcgcgag caggcacggc atttctgttt gtgccgatca acgtgatggc
     2761 cttctacttc gttcccaagg gaaatatgga caacgcgtcg ggcctaatca acctcgcgcg
     2821 caatgtcggc gcaagcaccg gcatatcatt cgttactacg atgttggatc gccgcgcaca
     2881 actgcatcaa aacattctgg cgcacaatat gcaaggcggc aaccgcctct accaagacgc
     2941 gctgaatcat ctggcgcacc tatttaccgc gcacggcagc gatgccgcac atgcgatcgt
     3001 ccaggcccac gccgtgctct acggccaatt gcaacggcag gccaccatgc tttccttcgt
     3061 ggataatttc aggaccatgg cgatcatctg tatttgcgtg atccctttga tgtttgtgat
     3121 gaagggcaga agaccacaag ccgagcaatt gcgcattcac taggcgcggc gttttcgcag
     3181 acctcagccc aatgtccgaa tcagctcttg cttactacag gagcgacctt gccccacgct
     3241 cccgtgaaga caaaagatga cgccggctta cggtcgcgca ctcccatctc gcgctggcgc
     3301 aacgggtcgg gcagcgaaga agggtcgccg gggtacccaa gagcgatccc agcgaccgct
     3361 tcgtgatcct cgggaatacc aaaaatctgg cgcgccttat cgggaagaat tcctgccatc
     3421 tggtgagcct gcaggccaag agccgtggct tgcagtgcaa gcgaagtggc ggcttgaccg
     3481 acatcgtgaa atgcgtgccg attctgcgct ccatcgtgcg gcatcttgtg agccgcgacg
     3541 ctcagcccga taaccggggc gtgcttcgcc cagccttggt tgaactccac aaacgaatcg
     3601 agaacttttt tgaaattgtc agggtcatcc ttggttgcaa caataaaaga ccacggctgc
     3661 gcgttgtagg acgacgccgc ccagcgagcg gcctcaaaca gggtgcggag tttttcgggt
     3721 tccacgaggc gcgcttcgaa tgcgcgcggg ctccagcggt ggctgagtaa gtcgtttatc
     3781 ggcacggctg ctggagcggg tttatcgcga tcagacatca ttcctccttc tgattggggc
     3841 aattttaacc acgaattgga tgcttagcta gcagactcgg cgccagagac tatttcaggg
     3901 gtggcgcggg aactaaaggc aggcggcgag gctcgccatg aaagcctcgt atttccacat
     3961 catcgagcag aagcgacggt gcaatcacgg tgctgggaat gccgccttgg gccgagccaa
     4021 aagctccgag agccgtgcca gccagattat ccgccggatt ttgcatgtag gtgtaaacgg
     4081 cggcgtcgtc gccgatcccc aggatattgc ggaaggatcg cagggtaagg ccctcgatca
     4141 cgcctccgcg caccggctcc tcatgtccgt ctgccacgta gacgcgggat acggtgagcg
     4201 gcatacgctc gccgctcgca atcccgccgg cgattgcgcc tacaaaatcg gtaaagtcct
     4261 gttgatggac cgacgagact acaggattgg ccattcgttt cacttcgaga caccattcgt
     4321 gaccatcttc tcgacaggca tcgataaatt tcttccgtag atcgtccggt ttcaacgtat
     4381 ccgacgattg aaagaaaagg ttgctgctta gcggcttggt atccatgagc agagccgagc
     4441 gcgcgtggcc attggaagcg gaaaaatcag gccccggccg ccgtgacatc aatagatttc
     4501 tcagaatacc gttttcgacg atgggaacac gctcgccttt cacgccttca tcgtccacat
     4561 catacgcacc cagcagtggc tggcccttaa actccgttgc ggtcggatca tcaaccagac
     4621 taacgctggc gggcagcact cgggtgccca cacggcccgt ccattcgctt cgtcctccaa
     4681 atcgttcgag aaacgcgttg agatcttggg tcatcgaaag tggcgggcgc gcgcccgaaa
     4741 gagatggttc gagcacctga gcaagagccg aacctgccgc cgatggatcg aaaagaactg
     4801 gcccggtata gtcgggcaca agagcacccg cgcgcagggt caaaagttct accgcagtct
     4861 gcccgagccc tttcgaaacc gctatgggcg caggtaggcc ggaaggttcg cttgaataaa
     4921 cggcatagta atcgttcagg ggcatcccgt catcggcttc agtatcgagc gcagcttcga
     4981 tagccgcgag atgccgcgat tcgcggatga tcgtaccttc gctcgtcatc agataagacg
     5041 taacgtaaat cagcgaataa ttcacccgcg tgccatagag ctgaggaaaa tcctttagcc
     5101 cggaagaagc tgcgcgggcc tcgtcttccc aatttcgcga agtccaatcc ggttcgattc
     5161 gcggatcgat tttcacaagc ggtgtggcct gcgagaagtc gtcaatctcc ggcggttttg
     5221 taaggctgcg aaggaacgcc tgtttgagag acatttgcgt tgcggcggcc ttgtatgcct
     5281 ggtctgtggc cagccacaaa tcttggcgca gcgacttgta gtcccggtcg atgccaactt
     5341 ggcctgttga tccgagaaat ccttgaaagc cgtcctcgct catgaagttc gaactatcaa
     5401 ggtgataatc ccctatgcgc acatccacgc tcatgaagcg gctgcgagat gtcgaactcg
     5461 ataaaagcgt gccaaaagac gccgtcacgc tgcgaatatc caaatcgagc aagcgatact
     5521 cgatgtaaaa gggcttttca acgcccggaa gagatagtcg cgtgcgtgcg cgttccattt
     5581 cgtcgtgcat cgcctgcagc gtcaggtctt tttcccctgg cggcacgggc gcttgcgccg
     5641 cataggccgc cggcatcgaa gcagcaacga gtaatgcgag caaagcgaaa agttttgtca
     5701 ctgctggggc ccctttgctt ccgggtcgtg cgcgggcggc ggcagaattg gcggccgatc
     5761 cgttgaagtt tgtttcttcg caaattccat ttcggaaatc aaaataccgg gcgcgacggc
     5821 cgaaaccggc actgaacctg attccgctcc gcaatagccg ttgaaaatct cctgtttgtt
     5881 ctcggtgcct acgattttcg tcagcgagac cagcggcgtt ccgacgatat cgacaccgcg
     5941 caccagctga tcggggcgcc catcggcaaa tactttgtat acgatgagtg gagtcacctg
     6001 aaatgcttgc ggctggccgc ggccggtaaa cgtgaatccg cccgcaatat cgtcgatcaa
     6061 caatccgtac ggcaggttct gctgcttcac aagagcaatt aatttccgtc gaagctcgcc
     6121 aaagggcacg ccgttcgagc tggtaacaat caggttcccc tggcgcgcta tggggacata
     6181 gccaagctgg cgccggccat gaccattcga gtgagggata tccatcagcg gcgaacgcga
     6241 catcagaaag gtcttcaata cgccgtgatc tacgagcgaa acatcctctg acggcacgcc
     6301 ttcgtcatcg aacggataat tgccgagcag catctggcca cccagagtgg cctcggtagg
     6361 gtcgtccttg atggagatga aatccggaag aatgtgttgc cctatcttgc tcgtgaaagt
     6421 ctggccttcg gtaatatctt tttggcgaaa gccttctgcg cgatgaccga aaacttcgtg
     6481 aaagaaaacg gcggcagcgc ggcccgtaag cagagcggga cccgcataag gctcaacgag
     6541 cggcgctttg tcgagagctt ccgtttcctc aatcaatcga tgaatcgagt tcgagacggt
     6601 tttgtcgtcg ggagcatcct tgggatcgag ccaatcgaag ttcgcatacc gctggatatc
     6661 catcccatcc ggagccttgc tttgaacgaa gagttcaagc cggtaatgaa tcagtccaaa
     6721 ggccagcttg gttccctcgg tgtttacttg atactggttc gtggcttgcg ccgtgaacgt
     6781 ggcgatggag ttcagcaccg ccggcgattg gctgaattcg gcggtatatc ttcgcacgcg
     6841 ctcctcccac ggcttgcgat tcacatggat ctcaacgcgc gggccaaccg acgtgtgggg
     6901 ttcctcatgc gaaaaatccg gcgcggcgcc ttccgccgtt tgcacctgca cttgctggct
     6961 ggtttttacc ttgatgagcg cttgggacgc cgcgcgatac tggcggtccg tttcacgcca
     7021 gatttcccgg cgcagtacag gaatgtcgtc atcgagcgcc acggtagtgc cggggctgtt
     7081 ccagctgggc ggccggtcac cgagtttgtg cgtgtcatcc agttgggcgg ttcccacgcg
     7141 tgtttgtact tccagccagc gcccacggtc ctccgagctt gaaagaagag cgccattcga
     7201 acccgaaact tgggcgtact cccggtcgga aaccgtatag ctgatgaaat aagccgatgg
     7261 atcggctttc ccgagggcgg ccatcgagcg atcgagttcc tcctgcatag ctgtcaaaac
     7321 cggcggggta gccggcgttg gtcggcccgc agcccagcat ccccaggctg ccgccagcag
     7381 cgccacacca atcgtgaaac ttctatgtct catttggact ctcatcgttg cacaaagtgt
     7441 aacagtttaa gataggatgc ggccgattcc caaaagaaaa cctgagttgt ttcgtccgtt
     7501 ggcaaaaaaa gtagtgccgg gccggttata atccggcgaa ggggaattag atgcgggctt
     7561 tggtagcgaa gaggtatggc gcgcccgaag ttttggaggc gcaagaggtc ccggatccac
     7621 agccgaaggc tggcgaagcg ttgatccgag taaagacgat tggcgtgaat tttgccgatc
     7681 tgctccagcg aatggggatc taccctggca cgccgaaacc tccgttcatt ccagggttgg
     7741 agctggctgg cgtggtggaa agagtggttg agggtcccag atcgccgggt ggcgatcctc
     7801 taaagccggg agatgccgtc gttgccctca cgaacttcaa cgcatacgca caatgggcgg
     7861 ccgtgccgtt gaacagagtc taccgcctcc ttccgggaat gggattcgag gatggagccg
     7921 ccatacccgg gaattacgtc accgcatatc acgccctgtt ctccatgggc aatttgcaac
     7981 ccggcgaccg cgttctgatc cacggtgccg cggggggagt gggaatcgcc gccgtgcagc
     8041 tcgcgcgcgc gaggggactc actatttttg gaacggccgg cccttccaaa caggaatatc
     8101 tccgcaaaat cggcgtggac cacgcgatcg actacgaaaa gaccgatttc gtgcagacgg
     8161 tgcggaaatt cgcgcctgac ggaattgaga tggtgatgga tccgatcggg gggaaatctt
     8221 ttaagcgcag ctatgagtgc ttgggtgccg gaggcaggct ggtggtctat ggcatgtccg
     8281 cggccgcggg accggatggg aagcgaagct tgattcgagg cctcacggcg ctgttacaaa
     8341 taccgcgatt taacccgctg gagttgatgc gccggggggt gtcggtgatg ggtataaatc
     8401 taggcgcaat ggaggcgcgg ccagcggtcc tgcgcggcga gctggaggag atttttcgac
     8461 tatacgccgc gggaaaaatc aaacccgtga tcgggaaaat tttcccgctg gatcaggctg
     8521 cggccgcgca tcgctacatc cacgaccgaa aaaatatagg gaaagtaatt ttgtcggtaa
     8581 agtagagagg aatcacggcc caacccgaat cgccgcctgt tttccaattc gcccccacga
     8641 acactaattg attttgctct tccttaccgt gtgtggtgcc ataggaacta ggaagcggtc
     8701 gcgccaataa ttggaggtac agatgcggag catcggcggc atcgtgggtc tcgtcgtcat
     8761 agccctaatc gcggggctga cgtacaaata ttatttcgcg caggggcaat ccgctggagt
     8821 gaccacacct cgacagacaa ttgacgtcgt gggagtcaaa aacgacttgg tcgccatcgg
     8881 ccaggccgaa cgcctctacc aagccgagca cggatcctat gcctccctcg acgacctcgt
     8941 ctcaagcggc gcgatgagca tgaagaaaac gggacgtgat ggttatacct atgaggccga
     9001 gacatctgcg gatggctttc gcattaccgc gaattgcgcc tccgatacgg ctcctgggtg
     9061 cacaagctac gcaatcgatc agaccatgga agtccaaact gctccctaga tattccttat
     9121 cagtttatag tcgaggccga tgagattcag cggcttgcat tcggcttggc tgcttcgtag
     9181 aacggtgaga gttcgcgcaa gcgcttaacg gtttcaatga cgcggcccgc tgcatactcg
     9241 atttcctcgt cggtattgaa acgccctatc ccgaagcgga tggaactatg cgcgagatcc
     9301 tccggcaccc cgagagcgcg cagcacataa ctaggctcaa gcgtcgccga cgtacacgcc
     9361 gatccacttg acacagccac gtcattgatt cccatcagga gtgcatcgcc ttccactccg
     9421 gtaaaactga cattcagatt attcggcaac cggtggatca tcgaaccatt aatggatgtg
     9481 ccgtccaaac tgcttgtgat cgcatccttc agtttgtcgc gcaggcgccg cgaccgctcg
     9541 ctctcctcgg ccatctcttt ctggcacaat tcacatgcct ggcccagacc aacgattccg
     9601 gggacgttga gcgttcctga acgcatgccg cgttcgtgcc cgccgccatc gatcatggaa
     9661 ctcaaattga cacgaggatc tttccggcgc acatacagga ctcccacgcc ttttggtccg
     9721 taaattttgt gcgctgaaat cgaaagcagg tcaatcccat ctttttgaat atcgatggga
     9781 atcttgccca cggcttgtgc cgcgtccacg tgaaacagga tttttttctc cttggcaatt
     9841 ttgccgatcg cttcaatagg ctgaatcact ccgatctcat tgttggcgta catgatggaa
     9901 atcagaattg tcttcggagt gattgcgccg cgaagatttt ccagatcgat taatccatcc
     9961 gagtgaaccg gaaggtaggt gatttcgctg ccagaaatct ccaggcgctt gcatgtgtcg
    10021 agcaccgcct tgtgttccgt cgcctgactg atgatgtggt ttcccttgcc gccgtatgcc
    10081 tccgccacgc ctttgatcgc gagattgttc gattccgtgg cgccgcttgt gaatatgatc
    10141 tcgcgcgggc tggcatggat caggcgcgcg atttggccgc gcgctttttc gacggcgtct
    10201 tctgctttcc agccgaattc gtggctgcgg ctcgccgcat taccgaattt ctcagtgaaa
    10261 taaggcagca tcgcatccag aacgcgggga tcgacggccg tggtcgcgtg gttatcgagg
    10321 tatacgggta gcttaagcgc catttgcttt attctagtcc gcccaccaca acgcgtccag
    10381 cgagggactt gccgctttcg cgcaagaaaa gcttctagtt tgccccacga gaagcttcta
    10441 gtttgccgca cgaaaagctt ctaactcgaa acaaattagc gctcctggcg ggccattttc
    10501 gacaccttgt gaccggtaaa cggggataga cgaatgggca tggctgcatt cgtcttcgtg
    10561 aggtcaaaaa tcgcgagaac ttatccccag ggcgcgctcc tgacggctca ctcctagaga
    10621 ttggagatct cagtgctgag gcgtccggcg gggcataggt tttttcgcac cacccgcctt
    10681 ttccggaatc acgcttgggt ccactttcac ctggtcccag tgcaaaataa atggagaagg
    10741 atcgtagccg accagcatgc ctgaaaatcg gatgggatca tctttcgaaa gtctgaccac
    10801 atcaggttgc ggtgcaacag gtggcagatt cgcggcgtta gcgtttggcg cgggggtacc
    10861 cgcaggcttc ggcgcggtcc aaactttcac atccatattg gccattgttg cagcttgcat
    10921 ttcgtcggaa ttggcgctgg tgaataccat gaaatcgacg taaccctcgg atttttggac
    10981 gtcgataatt ttgccgacga cttcaggaaa ctcagcgccg cagatagcca gccaagtcgt
    11041 tttcgctttg tcaccacctc cgctcaggtc ggcgatcacg gtaattatcg tgctggcctg
    11101 actgattttt gtcagatcgt cgtggcttgg aatcgtgtag gtggcggggc ggtcgggtga
    11161 attagccgcg agctgcagaa gctcgttcaa ttgcggatcc acctggctgt cacatcccgg
    11221 ttgctcgtac gcgaggattc tcgaacgaag gtagtccttc acctttgcgt cgtcgggaac
    11281 tttcaaatcg attgcgcgag ccagatacca aaatccgtcc agtggttggg gcggagtcgt
    11341 cgcaagatag gcaaggccca agcgataatc cgttacagca tccttgggat tgatagcaag
    11401 acctgctttg aaagcctcga tggaggcagg gtaatccttt aactgaagat ccgcagcgcc
    11461 tgctgccgct tcgaggaatg ctgtgccttc cttgacctgc tcatccgtca ccttcgaatt
    11521 gggcatcttc ttaagatcgg gaaaaagttt gactccgagc agcgccgcgt ctctttcctt
    11581 aacgagctga tcgtgagcat cgggagcctt tgcgtcgaaa gccaatccaa acagttgcac
    11641 gcgagcctgg agcgcttgca ctcgaagagc caagttcgct ttatcgaggg cgatcagctt
    11701 atcggcatac tcgatggcct tgggatactg cttcagctgg taataggtct ggtagtaaaa
    11761 ctgatagatg tactccatca gcgt
//
LOCUS       DATNDT010000420         7627 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1244810, whole genome shotgun sequence.
ACCESSION   DATNDT010000420 DATNDT010000000
VERSION     DATNDT010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7627
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1244810"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..321)
                     /locus_tag="VN788_13730"
     CDS             complement(<1..321)
                     /locus_tag="VN788_13730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU21639.1"
                     /translation="MNEMPPIPSAIEAPTHPAPPVLRITPPGRWWAIPFAELWENREL
                     IYFFVWREIKVRYKQTVIGAAWAVLQPFMSMLVFTLFFGRLAHIPSEGIPYPIFYYSA
                     LLPWM"
     gene            complement(327..1805)
                     /locus_tag="VN788_13735"
     CDS             complement(327..1805)
                     /locus_tag="VN788_13735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21640.1"
                     /translation="MNLTTSETESAEDAIRLRKSRKYEIALVVIFLLTLPLCNPWVRG
                     DGVGYYAFARSMLIEHRLDFANDWLHANESFRLGRTDAAGHINADQYTPTGHLDNHFS
                     VGPAILWSPFLILAHAGVVLYDHFGGKIAADGFSRPYLLAMALGTAVYGFLAVLISFS
                     LARRFVPEKWAFLGALGIWFGSSLPVYMYFNPSWSHAQAAFAVALFLWYWVRTRGHRT
                     WTQWVILGALGGLMMDAYYINAVLLLIPLCESAGLLWSAFKNNRPHTIRAFVLKNIAS
                     CVAIFLAFLPTLITKKIIYGSYLNFGYTEHWFWESPAFFRVCFSSEHGLFSWTPILAL
                     AVIGLVFLRKHDRTVALYLLMGFVLYVYAIGCYQDWHGISSYGSRFFVALTAVFIIGL
                     AAFFDWFAGLWRERRTAVYAATATTAILLLWNFGMIFQWGMHWIPERGPVSWRMAAHN
                     NVVAVPEQGASILRTYFIRRSTLMNRIEQRDIQQLKQSQGTK"
     gene            complement(1823..3406)
                     /locus_tag="VN788_13740"
     CDS             complement(1823..3406)
                     /locus_tag="VN788_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012099272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyltransferase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HXU21641.1"
                     /translation="MGTTPTHHPPERTEWSRGAAKGSATPHVSPDHRRASTTLSVIVP
                     AYNEQFLVEASLARLLILADSPLLDLVKIIVVDDGSSDRTHDALQCFRESIEAERGLN
                     KISWVWVRHEKNSGKGAAIRTGLLHVDTELVVIHDADLEYHPYDLLRMLEVFLAEDAD
                     AVFGSRFMAGGHKRALFFRHALGNKLLTFLCDLVCDLNLTDMETCYKMVRADLLKSIP
                     LESSTFDVEPELAIKLAKRGSRIFEVPITYSGRTYKEGKKIDWKDGARALWAILHYAI
                     SDRIYVEDESGGEILERLNRAPRFTQWMADVILPYVGDRVLEIGAGRGNMSVHLMPRS
                     VYWATDVNRHYLDYLATLRTTRPYMRVAHTNAMDPDTFPAGQSFDTVVCLNVVEHVQD
                     DVGTLENIRNVLAEGGRAIILVPCGPRLYGSLDEVLGHFRRYTKDQLTKVAEQAGFHL
                     EKMLEFNRPGVAGWWLNACVLRRRTFGLGQIRMLNVLTPLFRRIDSWLPLPPLSIIAI
                     LEKPRSSHIRSEPYPSAQL"
     gene            complement(3671..6472)
                     /locus_tag="VN788_13745"
     CDS             complement(3671..6472)
                     /locus_tag="VN788_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HXU21642.1"
                     /translation="MRPSPRIFALLVCLALAAPIGRGQTQVSTLVKASAAVRAGNGSL
                     SRSVVPAAQTRISAVLGREQLSYHATPKADGFRVENSGQGISAEFRSSGMALRNASGR
                     WGMRLARYGYPAAMHDAKSVRPHAEANRIEYSRGGLNEWYLNGPLGLEQGFTLSRTPG
                     KTNGPLTLALTLSGNLTATLDPGAVSLTLRNRGAAVARYSGLTAIDASGRELPAWLEL
                     ADNQLAIRVDDSRAKYPITVDPYVQAAVLTPTDSNALSGSGDVEAVSVSSDGSTIVVG
                     APSASYQAEGDNVGTAYVFVKPGTGWANTSSFAAELTYSDRHDDVLFGESVAVSGDGN
                     TVVVGSETSVAYVYVSSGSGWGSGCTAPCVLTESSRLTLTGFEATTVALSGDGNTIAV
                     GGAGNASLFVKPSGGWASSSPLDATNTLGGALGFGSYNLSLSADGNILVGGAANADIG
                     GNFEQGEVEGFFTTDRWLTTPGVHSFQLTASDGAANDGLGIAVGISGDGTTVVAGAET
                     VGGASFPGAAYVFVRPGTDWGGETETAKLLASESTYLFGNAVGISGDGATVVIGDFGS
                     SFPSSAGNALYAFVRPASGWASSSALHQTAKVQPGAIARNGLAISNDSATIVAANVAA
                     YVFTGSAAAPTASLSTSSLTFGNQPVGTTSATQTVTLSNTGTADLHVSAVSASASFTT
                     TTNCVNASPIAAGGNCSEDVAFAPGSVGSSMGTLTFTDDSGGTAGTMQTVSLSGTGVQ
                     ASSSTSIGSASPNPALLGQAVTFSFTVSPPIGDTLTPSGTVTVNASTGESCTGSAPSG
                     SCSITFSTAVTRTVSASYGGDTNFMSSTSSNISEQVVDFGLSVSPSSERIPPGHPATY
                     TLTATSANGFTGVVSLACGPLPPHASCTVSPGSLSLSGSTGTAGVTINLENKGTYTLT
                     FTGNDGALVRTATATLTVK"
     gene            complement(6599..>7627)
                     /locus_tag="VN788_13750"
     CDS             complement(6599..>7627)
                     /locus_tag="VN788_13750"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXU21643.1"
                     /translation="QEASLRHLITGGAGFIGSHLSEALLARGDEVFIVDDLSTGSVEN
                     IRHLKTHQRFHYFFDSITNKHLLAELVDESDVVFHLAAAVGVRLIVESPVRTIETNVY
                     GTQLVLDAASKKKKLVFTASTSEVYGKSDKVPFREDSDLVLGPTTKGRWSYAASKALD
                     EFLALSYWKERKLPVIVVRLFNTVGPRQTGRYGMVLPNFVRQAHEDAPITVFGSGQQS
                     RCFCDVRDTVEAILRLVSNDRAVGEVINIGASEEVSIERLAHFVKERTNSKSPITHIP
                     YDQAYEAGFEDMLRRVPCLEKLERLAAFRPATPLSEIIDRVSMSLVRSAPPHGAAIAN
                     AAAAASTS"
BASE COUNT         1726 a         2172 c         2244 g         1485 t
ORIGIN      
        1 catccacgga agaagcgcgc tgtaatagaa gatcgggtag gggatgcctt cggaaggaat
       61 gtgtgcgagg cgtccgaaga agagagtgaa aaccagcatg ctcatgaagg gctgcaaaac
      121 ggcccaggcc gcgccgataa ctgtttgctt gtagcgaacc ttgatctcgc gccagacaaa
      181 aaaataaatc agctcgcggt tttcccacag ttcggcgaag ggaatggccc accagcgccc
      241 gggaggagtg atgcgcaaga ctggcggcgc cggatgggtt ggcgcttcga tagcgcttgg
      301 aattgggggc atttcgttca tttcgttcat tttgttccct gcgattgctt cagctgctgg
      361 atgtcgcgct gctcgatgcg attcattaat gtgctgcgcc tgatgaaata ggtcctcaga
      421 atggaggcgc cttgctccgg gacggcgaca acattattgt gcgccgccat tcgccaggaa
      481 actggtccgc gctcgggaat ccagtgcatc ccccactgaa aaatcatccc gaaattccaa
      541 agaagcagaa tggccgtcgt ggcagtagcg gcatataccg cggtgcggcg ttcgcgccat
      601 aggccggcga accaatcgaa aaaggccgcc agtccgataa tgaagacggc cgtgagcgcc
      661 acgaaaaagc ggctcccgta cgacgagatt ccgtgccagt cctgataaca cccgatggcg
      721 tagacataca acacaaagcc catcaggaga tacagagcca cggtccgatc gtgtttgcgt
      781 aaaaatacca ggccgatgac tgcgagagcc aggatcggcg tccaactgaa gagtccatgc
      841 tcggacgaaa agcaaacacg gaaaaatgca ggggattccc agaaccagtg ctcagtgtac
      901 ccaaagttca gataacttcc atagatgatc ttcttcgtaa tcaaagtggg caggaaggcg
      961 agaaaaatgg cgacgcaaga tgcgatgttc tttagcacga atgcgcgaat tgtgtggggc
     1021 cggttgttct taaacgccga ccataaaagg ccggctgatt cacacagggg gatcaggagc
     1081 aggacagcat ttatgtaata agcatccatc atcaggccgc cgagagcgcc gagtattacc
     1141 cattgcgtcc acgtgcgatg gccacgggtt cgaacccaat accaaagaaa aagggccacg
     1201 gcaaaagccg cctgcgcatg agaccaggag ggattgaaat acatatacac cggcaaagaa
     1261 cttccgaacc agattccgag agcaccgaga aatgcccatt tctcgggtac gaatcgccgc
     1321 gccaagctga aagaaataag gacggcgagg aagccgtaca ccgcggtgcc gagagccatc
     1381 gccaagaggt agggccggga gaatccatcg gcggcgattt ttccgccgaa atgatcgtat
     1441 agcaccacgc ccgcgtgtgc gagtatgaga aaaggcgacc acagaattgc gggtcccacc
     1501 gaaaagtgat tatccaagtg gcccgtaggt gtgtattgat ccgcgttgat gtgtcctgcc
     1561 gcgtcggtgc gaccgaggcg aaaactctcg ttagcatgaa gccaatcgtt cgcaaaatcc
     1621 aaacgatgct cgatcaacat cgatcgcgcg aaagcgtaat agccgacgcc gtcacctcgc
     1681 acccagggat tacacaacgg cagagtgagg aggaaaatca ccaccaaggc gatttcgtac
     1741 ttccgggact tgcgcagacg tattgcgtct tccgccgact cagtttcact ggttgtgagg
     1801 ttcatggcgc cgcgtgaata ctctataact gcgcgctggg atacggctcg cttcttatgt
     1861 gagagctgcg cggtttttcg agaatcgcga taatggaaag cggcggaagc ggcaaccacg
     1921 aatcgatcct gcggaagagt ggcgttaaga cattgagcat tcggatttgt ccgagtccaa
     1981 atgtccttcg acgcaacacg caggcgttga gccaccaccc agctacgccc ggccgattga
     2041 actccagcat tttctccaga tgaaatcccg cctgctctgc aaccttggtg agctgatcct
     2101 tcgtgtagcg acgaaaatgc ccgagcactt catcaagaga gccgtagagg cggggcccgc
     2161 acggcactag tatgatcgcg cgaccgcctt cggcgaggac attgcgaata ttttcgagag
     2221 tgcccacgtc gtcctggacg tgctcgacca cattcagaca cacgaccgtg tcaaaggact
     2281 ggccggccgg gaacgtatcg ggatccatcg cattggtgtg ggctacgcgc atgtaaggcc
     2341 gcgtcgtacg caaggtggca agatagtcga gataatgcct atttacgtcg gtagcccaat
     2401 acacggagcg cggcatcaga tggacggaca tattgccgcg tccggcacca atttcgagaa
     2461 cgcggtcgcc cacataagga aggatcacgt ccgccatcca ttgcgtgaaa cgcggcgccc
     2521 ggttcagacg ttcgagaatc tcgccgccgc tttcgtcctc gacgtagatc cgatccgaaa
     2581 tcgcgtaatg gagaatggcc catagcgcgc gagcgccatc tttccaatca atttttttgc
     2641 cctctttgta ggtgcggcct gaataggtga tggggacttc gaagattctg ctgccgcgtt
     2701 tcgccaactt gatggccaat tccggttcga catcaaacgt ggagctttcg agcggtatgc
     2761 tcttcaacag atccgctcgc accatcttgt agcaggtttc catatcggtg agattcagat
     2821 cgcagacgag gtcgcacaga aaggtgagca gcttgtttcc aagcgcgtgg cggaagaaaa
     2881 gagcccgctt gtgcccacca gccatgaacc gggagccaaa aacagcgtcc gcatcctcgg
     2941 cgagaaacac ctccaacatc ctcaaaagat cgtagggatg gtactcaagg tcggcgtcat
     3001 ggattacgac gagttcggta tccacatgca gcagcccagt ccgaatggcc gccccttttc
     3061 cgctgttttt ttcgtggcgc acccagaccc aggagatctt gttcagcccg cgctccgctt
     3121 caatcgactc gcgaaagcac tggagggcgt cgtgcgtgcg atcggatgaa ccatcatcaa
     3181 ccacgatgat tttgacgagg tccaagagcg gggaatccgc caggatcaac agtcgcgcca
     3241 agcttgcttc aactagaaat tgttcgttgt aagcggggac gatcaccgaa agagtggtgc
     3301 tggcccgccg gtggtccgga ctaacgtggg gagtggcaga gcctttcgcg gcgccacggc
     3361 tccattcggt gcgttccgga ggatggtgcg ttggagtcgt gcccataatt ctcgcccctg
     3421 agtattgcac tctgccatca gttcatgcgg ttcaggatcg cttccagtgt aacgaaagtg
     3481 tctccgaacg tctccatttc attataggag cggtgctcaa accggcgcga ccaggctgcg
     3541 aacaaaaagg aacgggccaa ccggggtggc ggacagggca gcggcgaagg cggaactgcg
     3601 ggcgtcgagc ggacacacga gaccgcagag gccaagtgct caggcgatgt ttacccagtt
     3661 acggtgatcg tcacttcacg gtcaaggttg cagtagccgt gcgcacgagg gcaccgtcgt
     3721 tgcccgtgaa cgtcaacgtg taggtacctt tgttctccag gttgatcgtc actcccgccg
     3781 tgccggtcga tccagaaagg ctcagcgaac cgggcgagac agtacagctc gcgtgcggcg
     3841 ggagcggacc gcacgccagg gacactaccc ccgtaaaccc gttcgctgat gttgcagtga
     3901 gggtataggt cgccgggtgc cccggaggaa tcctctcaga cgatggcgag accgacaaac
     3961 cgaaatcgac cacttgctcc gagatgttgg acgatgtgct ggacatgaaa ttggtgtcgc
     4021 caccatagct tgcgctgacg gtccgcgtga ccgccgtcga gaacgtgatc gagcaggagc
     4081 ctgaaggggc cgaacccgtg cagctctcgc cagtgctggc gttgacggtc acggttcctg
     4141 acggagtaag cgtgtcgcca atcggtggcg atacggtgaa gctaaaggtt acggcttgac
     4201 ccagtagcgc gggattaggt gatgccgaac cgatgctggt tgagctcgat gcctgcaccc
     4261 cagtgccgct gagacttaca gtttgcatcg tgccggccgt gccgccgcta tcgtcggtaa
     4321 aggtgagcgt ccccattgac gagccgaccg aaccgggcgc aaaagcaacg tcctccgaac
     4381 aatttcctcc agcagcaata ggcgatgcgt tgacacaatt cgttgtggtg gtgaagctcg
     4441 cggacgcaga aacggcactc acgtgcaagt cagccgtccc ggtgttacta agcgtgaccg
     4501 tttgcgtggc actcgttgtg ccgaccggct ggttgccaaa ggtcaagctg gatgtggaga
     4561 gagaagcggt cggagcggcc gcagatccgg tgaaaacgta ggcggcaaca tttgcggcaa
     4621 caattgtggc cgagtcgttg ctgatggcga gcccgttacg tgctatggcc cccggctgaa
     4681 ctttcgcagt ttggtgaaga gccgaggaac tcgcccaacc gctcgcaggt ctcacgaacg
     4741 cgtaaagcgc gttgccggcc gaactcggga aggagctgcc aaagtcgcca ataactacgg
     4801 tggcgccatc accgctgatc ccaactgcat ttccgaagag ataggtgctc tcggaggcga
     4861 gtagctttgc agtctcagtt tcaccgcccc aatcggttcc cggcctgaca aaaacgtagg
     4921 cggcgcctgg gaaggatgct cccccgacag tctctgctcc tgcgacaacc gtcgtgccat
     4981 caccgctaat gccgactgca atgccgagcc cgtcattcgc ggcgccgtcg gaggccgtca
     5041 gctggaagct gtggacgccg ggcgtggtca accaacggtc cgtcgtgaaa aacccttcga
     5101 cttcgccttg ctcgaagttg ccgcctatat cggcgttcgc cgcgccgcct accaagatat
     5161 tgccgtcggc gctcaggctc aggttgtagc tgccgaaccc gagggcccct ccaagggtat
     5221 tcgttgcgtc cagcggcgac gaactagccc agccgcctga tggcttcacg aaaagagagg
     5281 cattacccgc gccgcccact gcgatggtgt ttccatctcc gcttagcgcc acagtggtcg
     5341 cttcgaaccc ggtcaatgtc agacggctgc tctcggtgag aacgcaaggt gcggtacatc
     5401 cgctacccca accggacccc gaactcacat agacatacgc cacactggtt tccgatccaa
     5461 cgacgaccgt attgccgtcg ccacttactg ctactgattc accaaaaaga acgtcgtcat
     5521 gcctgtcgga gtaggtcaac tcggcggcga aactagacgt gttggcccat ccagtaccgg
     5581 gcttgacgaa cacatatgct gtccccacgt tgtccccttc ggcttgataa cttgctgaag
     5641 gcgcgccgac gacaattgtg ctcccgtcgc tgctaacgct aaccgcctcc acgtcgccgc
     5701 tgccggaaag cgcgttgcta tcggtaggcg taagaacagc tgcctggacg taagggtcga
     5761 ccgtgattgg gtacttggcg cgagaatcat ccacgcgtat cgcgagctga ttgtcggcaa
     5821 gttcgagcca agcgggcagc tcgcgcccac tcgcgtcgat ggcggtcaat ccggaataac
     5881 gcgcgacggc cgcacctcgg tttcgcaaag ttaggctgac tgcccctgga tcgagcgtcg
     5941 cagtcagatt gccggaaagc gtcagggcca aggtgagtgg accgttcgtt tttccgggag
     6001 tacgagagag cgtaaatcct tgctccagcc cgagcggacc attgagatac cactcgttca
     6061 gtcccccgcg cgaatactcg atccgattgg cctcggcgtg cgggcgcacc gacttggcat
     6121 cgtgcatcgc ggcagggtag ccataccggg ccagccgcat gccccatcgc cccgatgcat
     6181 ttcgcagggc cattcccgac gagcgaaact ccgccgagat accttgtcca gagttctcca
     6241 cgcggaaacc gtctgccttc ggagttgcgt gatacgagag ttgctcgcgt cccaagacgg
     6301 cggagatgcg agtctgtgcc gcgggaacga cgctgcgtga gagcgagcca ttgccggcgc
     6361 gcactgcggc gctggccttg acgagcgttg atacctgcgt ctgcccccgg ccgatgggcg
     6421 ctgcgagcgc caaacacact agtagggcaa agatgcgagg gctgggtcgc atagtgctgc
     6481 ctccttgaat caccgcaggg gaccgctgcg gtcgcgaaga gttgccggag gatactctcg
     6541 tttcttttgt cttgcaacag agaagaaatg gaatgaactt cgtgcgccca ggcaaaacct
     6601 aagaggtcga ggctgccgca gccgcattgg cgattgccgc gccatgggga ggcgcggagc
     6661 gcacaaggct catgctgacg cgatcgataa tttccgagag cggagtggcc ggacgaaagg
     6721 ccgccagccg ctcgagcttt tccaaacatg gcacgcggcg cagcatatct tcgaagcccg
     6781 cttcgtaagc ctggtcgtaa gggatatgag taatgggcga tttgctgttt gtacgttcct
     6841 tgacaaagtg ggcgagccgc tcaattgaaa cttcttcgct ggcgccaatg ttgatgacct
     6901 cgccgacggc gcgatcattc gagacgaggc gcaggattgc ctcaacggtg tctcgcacgt
     6961 cgcaaaagca gcgtgattgt tgcccagaac cgaaaaccgt gattggcgcg tcttcatggg
     7021 cctggcgtac gaaattggga aggaccatgc cataccggcc cgtttgccgc ggccccacag
     7081 tgttgaataa ccgaaccacg attaccggca gcttcctttc cttccaataa gagagcgcga
     7141 gaaattcgtc gagggccttc gatgcggcgt agctccaccg gcctttggtg gttggcccca
     7201 gaaccaagtc cgagtcctcg cgaaaaggaa ccttgtcgct ttttccgtat acttcagaag
     7261 tggaagcggt aaacacaagc ttctttttct tcgaagcggc atctagaaca agctgcgttc
     7321 catacacgtt tgtctcaatc gtgcgaacgg gactctctac gatgaggcgc acgcccaccg
     7381 cagccgccag atgaaaaacc acatcggact catcgacaag ttcggctaaa aggtgcttgt
     7441 tggtgatcga atcgaagaaa taatggaatc gctgatgagt cttgagatgc cggatgttct
     7501 cgacgctgcc cgtggagaga tcatcgacga tgaatacttc atcgcctcgg gcaagcagcg
     7561 cctcggacaa atgcgatcca ataaagccag cccctccggt tatgagatga cgcaatgacg
     7621 cctcctg
//
LOCUS       DATNDT010000421        10716 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_949075, whole genome shotgun sequence.
ACCESSION   DATNDT010000421 DATNDT010000000
VERSION     DATNDT010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10716
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949075"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..695)
                     /locus_tag="VN788_13755"
     CDS             complement(150..695)
                     /locus_tag="VN788_13755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21644.1"
                     /translation="MGAHIDSLGRKTAAGKWLWFVTLTFRTPHYPWMHGFPIEQPQPS
                     PDFVHHFFASMIRWLEREVHSHVDYFVADQFGEAGGRIHLHCGLSWPGLFDYRWKPLQ
                     EMLWKKAGFNRILPWEQNAAFYIGRYIGRDAARAHWDWNVGAREPIRRVSPVGRKLIV
                     TSLAPDDSSSAYRQTAGTWHR"
     gene            complement(3182..4120)
                     /locus_tag="VN788_13760"
     CDS             complement(3182..4120)
                     /locus_tag="VN788_13760"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HXU21645.1"
                     /translation="MGVEWSGFGNSVQPPLSVQDISRVASRDLVLIHAFDSEGHERSV
                     GIGFFVASDGTTVTNYEVIRGASRASAEFGSGISADVLGVIAYDTERNIAIIRVAARP
                     KPTVELGDSDEVKIGENLIVLGSAMDSQNALSEGVVSGMRNGVIQINNPIAPGCSGGP
                     VFDSQGRVIGMATAAENSGFVVPIDWVKPYLSADTLRPLSAVAAENMVVDDLLDGSIT
                     VPASDSKNFTITYDPNSMSDAEIQGHVTSTGGFGGKITISIYHQGQPIYQCRDTACDV
                     HQAISAAGDYVVMIDNRESPMFSRTVSGNISMRYVK"
     gene            complement(4670..4855)
                     /locus_tag="VN788_13765"
     CDS             complement(4670..4855)
                     /locus_tag="VN788_13765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21646.1"
                     /translation="MSYAQVAGAVGIYPIRQVARDLQRYASFGYVARYKAAGKFRYEI
                     TRRGRERLVFFNRIGKK"
     gene            complement(4896..5588)
                     /locus_tag="VN788_13770"
     CDS             complement(4896..5588)
                     /locus_tag="VN788_13770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21647.1"
                     /translation="MRNRLILCVGFRGSGKSTLAAGILRKNNGVFVFDPHVDTAYSWI
                     PNTARTIEQLDDYSRWRREAKPNRVALRYVPDGRLDPYDALNDFCAWVWGWRNVWVGF
                     EEISESTRSSSAAGMPPELRRIVNQGRHKGINQVYCGLRYQEIPRPISAGADVQILFR
                     SREPLDLDAMRSRIGSDATERVEGLGPHEALVFFPDRTWQTVGSRDADIAELVLRDTA
                     EDVQADVQKELA"
     gene            complement(5590..5781)
                     /locus_tag="VN788_13775"
     CDS             complement(5590..5781)
                     /locus_tag="VN788_13775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21648.1"
                     /translation="MSEQAYMPIRYSSGTENVPNTGKRGLGWLWWAWIGFVVLLFLAW
                     RSGWLEVSVASVANPNGWK"
     gene            complement(6113..7147)
                     /locus_tag="VN788_13780"
     CDS             complement(6113..7147)
                     /locus_tag="VN788_13780"
                     /inference="COORDINATES: protein motif:HMM:TIGR03435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03435 family protein"
                     /protein_id="HXU21649.1"
                     /translation="MDKQTTAAQIALAEVRVPTAVPDYGTRTPNVLSRRILSRRISGL
                     PAKRVYNRFAMRSRMAHTLALAFATALLMLAAGLFPSHILFAQSQQTQTQQPEAALPS
                     FDVVSIKPDNSGWGGMNSTPGLVPHISIINIRLDNLIESAYGVRDFQVFGAPGWIKTK
                     RYDIKATIDESLLQQLRKLSGDEQVQQRQRMMQSVLADRFKLRVTHATKQFPAFNLVL
                     LNDTAKLKPFAVDPFPRTPTGLVFISLGRARGDQVELIKANLSQLTVGLTTALAEPVM
                     DHTGVTGNYSLKLRWTDASQLPAAATTPDPDFDRTLAEALRELGLKLEPTTAARDTIT
                     IDHIEEPTPN"
     gene            7310..7792
                     /locus_tag="VN788_13785"
     CDS             7310..7792
                     /locus_tag="VN788_13785"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HXU21650.1"
                     /translation="MNSPSQHERHMRRCIELARQAIASGDIPVGSLIIDGEDVVSEGV
                     EAVRARHDATAHAEMEALRTAFARRRSHDLTGCTLYTSVEPCIMCAYAIRLARISVVV
                     CGARGGDEAEDISGHIVLTDERVAPNRPTPLVVRDVLMQECQSVRDQSRATSSTGTKI
                     "
     gene            8048..8269
                     /locus_tag="VN788_13790"
     CDS             8048..8269
                     /locus_tag="VN788_13790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21651.1"
                     /translation="MDDLERCEETLREVESKLQGDISSLPSTDSIERTHMSEALGFIA
                     KAIVAVSSARNEIAALPAYEVRYRTPNEV"
     gene            complement(8292..8747)
                     /locus_tag="VN788_13795"
     CDS             complement(8292..8747)
                     /locus_tag="VN788_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21652.1"
                     /translation="MRFAGALLLAILVLSSIPSRSAPVCIPFRTGRAGVVEVNCQMQS
                     NPRRILSCVIDTGAQLSMASLSSVSSKSMRGAPVITFQTPGGNQPAYETKQTILLGGI
                     PILATVEVTPQISAIGTDVLIGEDVLSQFQRVSIDYKNHVLTCETELPE"
     gene            complement(8900..9484)
                     /locus_tag="VN788_13800"
     CDS             complement(8900..9484)
                     /locus_tag="VN788_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HXU21653.1"
                     /translation="MPIDTVDLARYLIGKTIVRKIGRNKLSGRIVETEAYPPGDQAGH
                     AYSGRTARNQSLFLAAGFAYVYFIYGTSYMLNVSAEAPGVGAGVLLRAIEPIDGIEIM
                     KRLRGTAKVTDLARGPGRLAAALQIDLRLDGVDLCADGPLWLGTAVRDPARILTSPRV
                     GITREVERPLRFFEAASPFVSRPYPISPPVKNTS"
     gene            complement(9511..>10716)
                     /locus_tag="VN788_13805"
     CDS             complement(9511..>10716)
                     /locus_tag="VN788_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HXU21654.1"
                     /translation="SLACLLLSFVFAVVPASVSAAPQQAGDHWVATWMASPMQGTWAD
                     GFQNQTVRMIAHISLGGSRVRVEFSNAFGAQSLTIGAAHVGIQSNGAAIVSGSDRALK
                     FNGSTSVTIPPGALEVSDPVDLTVAPLSSLAISVFVPEQTEPATYHASGLHTTYISGP
                     GDFTADQDMHSQWTTASYYWLSGVDVEAGADAAAIVALGDSITDGAHSSLNANMQWPS
                     QLAERLQKNPATAHIAVANAGIGGNRILHDVSGPNALARLDRDVIARAGVKYVIVLES
                     INDLGWPHEAASHGTQEVTAEQLIGGLRQIIDRAHAHGIKVFGATLTPYGGANYFTDE
                     GEQKRETINQWIRTGGAFDGVVDFDAAVRDPNNPKQFAPANDSGDHLHPNDTGYKAMA
                     NSIDLSLFR"
BASE COUNT         2407 a         3126 c         2857 g         2326 t
ORIGIN      
        1 cgccgcagaa gacacctcgc tagccgcgtg cggcaccccg tccaagttcc acggcaagtg
       61 cgcgaaccat ccccatgctg catacgcagc aaaaacgcgc ggttcaatgg cgaccggact
      121 accgaacatc gggccgctcg aaatagtgct taccgatgcc atgttcccgc cgtctgccga
      181 tacgcgctgc tcgaatcatc gggcgctaaa gatgtgacga ttaatttccg tccgactggc
      241 gatactctgc gaattggctc acgtgcgccc acgttccaat cccagtgagc acgcgccgca
      301 tcgcgtccga tgtagcgccc gatataaaaa gcagcgtttt gctcccacgg taagattcga
      361 ttgaatcccg ctttcttcca cagcatttct tgcaagggtt tccagcggta atcgaaaaga
      421 ccgggccacg aaaggccgca atgtaagtgg atgcgtccgc ccgcttcgcc aaactggtcc
      481 gcaacgaaat aatcaacatg gctgtgaacc tcacgctcta gccatcgaat catgctcgca
      541 aaaaagtgat gaacgaaatc cggcgacggc tgaggctgct caatgggaaa gccatgcatc
      601 cacggatagt gaggcgtccg aaacgtgagt gttacgaacc agagccactt tccagccgcc
      661 gtttttctgc cgagactgtc aatgtgtgcg cccatacgat cagtgaagca acgaccacag
      721 ccacaattcg gaccatgctt ccggcgtctc aatcgccgac aacgatcaca gccgcaattc
      781 acgcggaatg cccctgtacg ttcacagaca cgaggccgca agcgagcatc gggataagat
      841 acattcagtc tttggccatc atattgaatc tccggctgct tagctttttg acccataccc
      901 gctttgcccc ttcgttcaga gacaccctgt ggaatcaaga atgaaacccg ccttccggct
      961 ccactctcgc gtaccagccg ccgccgacgc acgcccgact gtctaggccg tcgtagtctt
     1021 ctgttgcggg gcttgtttgc ttcactcggg cagcaaaaac cgctgccctc gttcgccttc
     1081 gctcgcggcc agcaaaaacc gctggccgct cgcttcggac aaactctttt catccctacc
     1141 ggcgtccgag cgaaaactct ctgcgctaca ctaaccgcta ggtattggag aggggccacg
     1201 cgccgtttag gtcgagagtc gaatggtttg tcattccttc ggtgctcccg ctcgtgctcc
     1261 gtggccccgc tgcaatacct gctctggcga attgaaaagc caactggcct tgcataattc
     1321 caccgcatga ccgtaggcgc ttcgcggcaa cacgtcggca aatcaagcac gtcggttcgt
     1381 cgccgttgta agaaacccgc acaaaactct ggtaacgccc gcacagagtg cgcgacagtt
     1441 cgcgtggcaa gtgaaccttg cgcgacaaac cctctgtcgt cctcacggtt gcgttttccg
     1501 cgcagaggca tgaaagaggc agcgagtatc gtcgcctttc tcctgtccaa aaagaatgtc
     1561 cgcctcggaa aagaaatcga gcacggactc aggatgcttc ctgctaatgt gagctattac
     1621 ctgttccttt ggcgatttca cgtaaatcga gccgcgcggt gtatggccgc agagcctaca
     1681 atgaactcgc ttcgatgaag tatccttgac gcgcttagca gctacgtcta cactctccat
     1741 gttcattcct ttgaacgaca atttttgggc gaggcttcca acctcgccct ttttcgtttc
     1801 cgcaatcaaa aaggcggcca gccgcgccga cggcgcgttt cggtctgctt cgtagactcg
     1861 tccgatgccc cggttttgtt ttcatccgta ctctttgcag gttcgctctt cggcggcttt
     1921 gcctctaatg ctgcaagtcg cttttcgagt cctgccatcg tttcggattg acgcgctacc
     1981 gtccgagaaa gtacgtcaat gtcctctccc attcggttgt agtgcgagag aactttttca
     2041 agcgcacgcg ccctgcttgc tgcctccatc ccctctgata gcgcagtcaa atcattttgc
     2101 cgaacgtcta gggtcttctc tgtttcagcc atgtttttcc tctctgcctc tcataaccgg
     2161 gagaagcacc ggtataaatc cctgctttta ttgccgaccg cgaagtcgcg cacgaagttc
     2221 ccggatgttt ctcttaattc cggcccggag atgctttgga gtagatttcg acttcgcggc
     2281 ggcgacccaa ccccgtagcc gaagttctaa gctgttatga atccctcttg ccattgcttt
     2341 gctttgcgcc tcctttcgcc aatctcaagt attggcgaaa gtctctcacg ggaataaaat
     2401 gtatttggac tttttggggt gaaaatggca ggccgtccga aacgcagctt acttgaattg
     2461 gaagtgctat tagcagcgga agctcaatgg gaaaagattt tgcaaggtct gcggcagggg
     2521 ataagaagcg ctgataacag tctgcccgat ctcggtccaa tagtagcgaa gtggcccgcc
     2581 agtaagccta tatccaacac agaaacgcgg ctcgcttacg agaagaaccg cttggtgtgg
     2641 ctaaatgatg acatcgaaaa agcggtctgc gcaccgccgc gggattttcc cccggaaccc
     2701 gagcattggc gtgcgctttc aaaacggtat acaacggcca gcgagattcg ctcagtctgt
     2761 gaacagtccg caattttgcg atgggtgaac gacgaaacgg cgataatcgc aatctacaac
     2821 atcccgccga gaggttggat caaaagcaag attcagtcaa tccgcgaacg ctatcaggat
     2881 acgggagcac tctggcaaga gtttggtagc tcactctacc gacatgccgg gagattctgt
     2941 gctgctaaaa aagatgcacg ttatccccgt tcaaaaaaac ggaaatccag cgagggcaaa
     3001 cgagccaagc atctcgcgcg agtgctcgcg gggtttacga ttctgccgcc gaatcgttcc
     3061 gggagtacag ccgttgacat cttgcgaaag gtatcgcgcg ggaaaatacc ccatagggcg
     3121 taacactacc aacgcggccc cttttcacca gcaatgtttc tttgacattg tggcctttac
     3181 tttacttcac gtaccgcatg gagatgtttc cgctgactgt gcggctaaac atcggagatt
     3241 cccgattatc aatcatcacg acgtaatcgc cagcggcaga aatcgcctgg tgtacatcac
     3301 acgcggtgtc ccggcactga taaattggtt gcccctgatg gtagatcgaa atcgtgattt
     3361 tcccgccaaa accacccgtc gaagttacgt gcccctgaat ctcggcatcg ctcatcgagt
     3421 ttggatcata cgtgatggtg aaattcttgg agtcacttgc agggaccgtg atcgagccgt
     3481 ctagaagatc atcgacaacc atgttttcgg cagcaacagc acttagcggc ctaagagtat
     3541 cggcactcaa gtatggtttt acccagtcga tgggtacaac aaatcctgaa ttctcggcag
     3601 ccgtggccat gccgatcacc cgtccctgag aatcaaatac cgggccaccg ctgcaaccgg
     3661 gagcaatcgg gttgttgatt tggataactc cgttgcgcat tccacttact acgccctctg
     3721 atagcgcgtt ctgcgaatcc atcgccgaac caagcactat gagattctcg ccgatcttca
     3781 cttcgtcgga gtcgccgagt tcgactgtgg gctttggccg cgctgccacc ctaataatcg
     3841 ctatattgcg ctccgtgtca taagcgatga cgcccaacac gtccgccgag atgccactcc
     3901 cgaactcagc agaggctctg gatgcaccgc gaatgacctc atagtttgtt accgtcgttc
     3961 cgtccgaagc cacgaaaaag ccgataccaa cacttcgctc gtgcccttcg ctgtcaaacg
     4021 cgtgaatcaa aactaagtca cggctcgcca cccgcgaaat atcttgcact gacaaaggcg
     4081 gctggacgct gttgccgaag ccggaccatt cgacgcccat aaaaaccaat cccgcgatca
     4141 caaccagagc aagagcaatc cagccgataa gccccagtga ccgccacttt ctagcaggat
     4201 caagcgagcg accacactct aaacagaatt gagaatcatc gggaagttcc gttttgcact
     4261 tgggacagaa cacgagccgc ctcctctgga atattggtct cagccaaaaa cgtggattac
     4321 tcgtatgtat gtggttccac cacgaaagtc aatatcttta gtactctcag taacgttgct
     4381 gtttagggaa aagtgctcga tacaggctgg atgaagcgta ccgttcaagt gaatgtaaag
     4441 atgacacaag acgactttga tcttttgcag cgggccgcac agaaacgctg gccagatgcc
     4501 gttatgacca attcaggcgt ggtacttgcc ttcgcaaaaa tcgcggctcg ggacacttta
     4561 aaggaaggaa aacctatgcg ctaaagaaaa acacccaaaa gcaccggttt gcggagcaca
     4621 cgtcagaaat taagcaaaag aggctagcag attgttttct tttttggttt tatttttttc
     4681 cgattctatt aaagaaaacg agtctttcac gtccgcgcct tgtaatttcg tagcgaaatt
     4741 tgccggccgc tttgtaccgc gccacgtaac cgaagctcgc gtaccgctgc aagtcccgtg
     4801 caacctgccg aatgggatag atgccgaccg ccccggccac ctgagcgtag ctcattgggc
     4861 tttgcgagcg agccaaagcg cccagtatcg cggccttacg ccaattcttt ttgaacgtcc
     4921 gcctgtacat cttccgccgt atccctgagc accaattccg ctatgtcggc gtcccggctc
     4981 ccgaccgtct gccacgtccg atccgggaag aaaaccagtg cctcatgcgg tccaagccct
     5041 tccacgcgct ccgtcgcgtc tgagccgatc ctagagcgca tagcgtccaa atccagaggc
     5101 tcccgcgagc gaaagagaat ttgcacatcc gcacccgccg aaatcggacg cggaatctct
     5161 tggtagcgca aaccgcaata cacctgattg atgcccttat gccgtccctg attcacgatg
     5221 cggcgaagct caggcggcat ccccgctgcg ctggaactgc gcgtagattc ggaaatttcc
     5281 tcgaagccta cccacacgtt gcgccagccc caaacccacg cgcagaaatc gttcagcgcg
     5341 tcgtatggat caagacggcc atccggcacg tagcgcagcg caacccgatt gggcttggct
     5401 tcgcgtcgcc agcgcgagta atcgtccaac tgttcgatag ttcgcgccgt gttgggaatc
     5461 cacgaatagg ccgtgtccac gtgtggatca aaaacaaaga cgccattgtt ctttcgcaga
     5521 atgcccgccg cgagcgtgct cttgcccgat ccgcgaaagc ctacgcacaa aatcaaacgg
     5581 tttcgcattt cacttccacc cgtttggatt ggcaaccgac gccacgctca cctcaagcca
     5641 acccgaccgc cacgcgagaa atagaagcac tacaaagccg atccacgccc accacaacca
     5701 acccaggccg cgcttccctg tgttcggcac gttctcagtg ccactggaat agcggattgg
     5761 catgtacgct tgttcgctca tcgcttttca ctgccccttt tcgacaaaaa actaggcatc
     5821 atttaggcat caaaatccgg gtttttgatg ccgtttttgg gcttgcgatg ggataaggcc
     5881 aactgattga acaagactta gagtgtgtac tgcaaattca aatcccatcg ctcaccccaa
     5941 tttcttcaaa aagcttccaa cctattcgag cgcgtaagtg caaacgcccg caccctttgc
     6001 aaaggaaagg acgcaaacgg tgccgcaccc tcgggttttg atccagatac taagatcgat
     6061 ccggcgttga cgcgcgtttt gagccgccgc tgcgccacgc gcccactcca acctaattcg
     6121 gcgtcggctc ttcgatatga tcaatcgtaa ttgtatcgcg tgccgccgtc gttggttcca
     6181 gttttagccc aagttctcgc agcgcttcgg ccagggtgcg atcgaaatcc ggatcgggcg
     6241 tcgtcgcggc ggcgggaagc tggctcgcat ccgtccagcg gagtttaaga ctgtaattgc
     6301 ccgttacgcc agtgtggtcc attacgggct cggcaagggc cgtcgttaat cccaccgtaa
     6361 gctggctcag gtttgcttta attagttcga cttggtcacc acgggcccgc ccgagcgaaa
     6421 tgaatacgag ccccgtcggt gtacgcggaa acggatcgac ggcaaatggt ttcagcttcg
     6481 cggtatcgtt caaaagaaca aggttgaaag cgggaaattg ctttgtcgcg tgcgtcactc
     6541 tcaacttaaa acggtcggcc aacaccgatt gcatcatccg ctgacgctgc tggacctgtt
     6601 cgtcaccgga aagcttacgc agttgttgga gcagcgattc atcgatagtg gctttaatat
     6661 cgtatcgctt cgttttgatc caaccagggg caccaaaaac ttgaaaatcc cgcaccccgt
     6721 aagcgctctc tataagattg tcgaggcgga tgttgattat tgaaatatgc ggaacaagcc
     6781 ccggtgtcga gttcattccg ccccacccag aattgtcggg tttgatcgaa accacgtcga
     6841 acgagggcaa tgccgcttcc ggctgttgag tttgtgtttg ctgcgactgc gcgaagagga
     6901 tgtgtgaggg gaacaaaccg gcggccagca taagcaatgc ggtagcaaac gcgagcgcca
     6961 gggtgtgtgc cattcgactt ctcatggcga agcgattgta cacccgcttt gctggaagtc
     7021 ctgagatacg gcgcgacagt atacggcgcg acagtacatt tggagttcta gtaccataat
     7081 cgggaactgc cgtcggaacc ctcacttccg ctagtgcgat ttgtgcagct gtggtctgct
     7141 tgtccaaatc cgcgactccg cgtcccaaga caaggacatt gtggaacaga aatcccatag
     7201 caatcttgtt caattcaggc ctactttacc cggcttcgcg ttggacgacg atgcctcgac
     7261 ctgcatcacc gatctcaagg ataattagag agatcgcgtt tctgaatcta tgaacagtcc
     7321 gtcacaacac gagcggcaca tgcggcgctg cattgagctt gcgcggcaag cgattgcgag
     7381 tggcgacatt cccgtcggct cgctgattat cgatggcgaa gacgttgtct cagaaggtgt
     7441 cgaagcggtg cgtgcgcgcc atgatgcgac ggctcacgcg gaaatggagg cgctacgcac
     7501 ggcgtttgcg cggcgacgtt cccacgatct aaccggctgc accctctaca ccagtgtaga
     7561 accgtgcatt atgtgcgcgt acgcaattag gctggcgcgc atcagtgtcg ttgtttgcgg
     7621 cgctcgcggc ggtgacgaag cggaagacat cagcggccac atcgtgctga ccgatgagcg
     7681 cgtcgccccg aaccggccca caccgctggt ggtccgcgat gttctcatgc aggaatgcca
     7741 gagcgtgagg gaccagagcc gcgcgacctc aagcaccggc acgaagatat agagaaggcc
     7801 atactcgcaa ccgaaagtac gcgttcaccg ctttcatcgc aaagtgccgc gtgcgcgtgt
     7861 cgcggggccg tgaagacggt ctccacattc gcccgcaccc atcaaatctg catccaacgc
     7921 agggcatagg gtaattctct cactcctcct tcaggctctt cccttcagcg attccccttc
     7981 tggcggccaa ggacaccgcg cggtacgttt atggcgtggg gaaacgggca taaaggaggc
     8041 ttcggccatg gacgatcttg aacgctgcga ggaaacgcta cgcgaggtag aaagcaaatt
     8101 gcagggcgat atttcatccc taccctccac cgactcgatt gagcggacac acatgtccga
     8161 agcgctcggc tttatcgcta aagcgattgt cgcagtctca agcgcgcgga acgaaatcgc
     8221 cgcgctgccg gcgtacgaag tacgctatcg gactccgaac gaagtctaag ctctaccgac
     8281 ggtatacatt gctattccgg cagctctgtc tcgcaagtca gcacatgatt tttgtagtcg
     8341 atgctcacgc gctgaaattg cgacagcacg tcttcgccga tcaggacatc cgtaccgatc
     8401 gcgctgattt ggggcgttac ctcgaccgta gcgagaatgg gaatgccccc tagcagaatc
     8461 gtttgcttcg tctcgtacgc aggctgattt ccgccgggtg tctggaaggt aatgaccgga
     8521 gcaccgcgca tcgatttgct cgataccgac gaaagagacg ccatcgaaag ttgggcgccg
     8581 gtatcgatga cgcaggagag aatcctccgc gggttcgatt gcatctggca gttcacttca
     8641 acaacgccgg cgcggccagt gcgaaacgga atgcaaacag gtgcggatcg cgacggaatc
     8701 gacgacaaga ccaaaatagc gagcaatagt gcacctgcaa atcgcattgt gaccgtccat
     8761 tcgtgtcgtt gaagctggtc actttgacgc ctcttcggct tattcgtaag caccggcggc
     8821 ctggagaata ggcaatgttt ttggcagttc gccagccccg tgaatttgac cttgaacgtc
     8881 catggtttaa ggagcgcgat taagaagtgt ttttcacagg tggtgagatt gggtaagggc
     8941 ggctgacgaa cggactggct gcttcgaaga agcgcagcgg gcgttccact tcgcgggtga
     9001 tgccaacgcg cgggctagta aggatgcgcg cggggtctcg aacggcggtg ccgagccaca
     9061 gcgggccgtc ggcgcacaga tcgactccgt ccaatcgcag atcaatttgc agtgcagccg
     9121 cgaggcggcc tgggcctcgt gctaaatccg ttactttagc cgtgccgcgc aggcgtttca
     9181 ttatttcgat cccgtcaata ggctcgatcg cgcgcaacag cactcctgcg ccgacacccg
     9241 gcgcttccgc ggaaacgttg agcatgtatg aagtgccata gatgaagtag acgtaagcga
     9301 acccggcggc caggaataga gactggttcc tcgcggttcg cccactatac gcgtggccag
     9361 cttgatcgcc cggcggataa gcctctgtct cgacaatgcg gccgctaagc ttgtttcggc
     9421 ctatcttgcg cacaatcgtt ttcccgatga gataacgggc gagatcgacc gtgtcaattg
     9481 gcaaactcgc ccgcgatagc cgccgtatcc tcaacggaag agcgagaggt ctatgctgtt
     9541 ggccatggct ttgtagccgg tgtcattggg gtggagatgg tcgccgctgt cgttggcggg
     9601 cgcaaattgc tttgggttat tggggtcgcg gacggcagcg tcgaagtcaa ctactccgtc
     9661 aaaggcgccg ccggtgcgaa tccactgatt gatcgtttcg cgcttttgct cgccctcatc
     9721 ggtgaaatag ttcgcgccac cgtagggagt caacgtggct ccgaacactt ttatgccgtg
     9781 cgcgtgagcg cgatcgataa tctgccgcaa gccgccgatc aactgctccg ccgtcacctc
     9841 ttgcgtgccg tgcgaagccg cttcatgcgg ccagccaaga tcgttgatgc tctcgagaac
     9901 gatcacgtac ttcacgccag ctcgcgcgat cacgtcgcga tcgaggcgag ccagtgcgtt
     9961 cggccccgag acatcgtgca aaatgcgatt gccgccaatg cctgcatttg ccacggcaat
    10021 gtgcgcggtc gcaggatttt tttgcagacg ctcggcaagc tggctgggcc actgcatgtt
    10081 cgcgttcaga gacgagtgag cgccgtcggt aattgaatcg ccgagcgcga caattgctgc
    10141 agcatccgcg cccgcctcca catccacgcc ggaaagccaa tagtaagaag ccgtggtcca
    10201 ctgcgagtgc atgtcctgat cggccgtgaa atctcccggg ccggaaatgt aagtcgtgtg
    10261 caggcccgac gcatggtacg tggccggctc ggtttgctca ggaacaaaaa cgctgattgc
    10321 gagactgctc agtggagcga cggtgagatc gaccggatcg ctgacttcga gtgcgcccgg
    10381 aggaattgtg accgaagtcg agccgttgaa tttcaaggcg cggtcagaac ctgatacgat
    10441 tgcggcgccg ttcgactgaa tgccgacgtg cgcggcgcca atcgtgagcg actgcgcgcc
    10501 aaacgcgttg gagaattcta ctcgcactcg gctgccgccg aggctaatgt gcgcgatcat
    10561 gcgcaccgtc tgattctgaa aaccgtccgc ccaggtcccc tgcatcggag aagccatcca
    10621 tgtggccacc cagtgatcgc cggcctgctg cggcgcggca gacacggacg caggaacgac
    10681 agcaaaaaca aacgacagca gtaggcacgc gagcga
//
LOCUS       DATNDT010000422         3202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1245344, whole genome shotgun sequence.
ACCESSION   DATNDT010000422 DATNDT010000000
VERSION     DATNDT010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245344"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VN788_13810"
     CDS             complement(<1..795)
                     /locus_tag="VN788_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HXU21655.1"
                     /translation="MHIRKQRLLTPGPTPLLPSALHAMMGSDIHHRTEDFRKIYRSVL
                     TDLKEVFGTSNDVLSITSSGTGALEASVTNFFSPNDKVIVCSAGKFGERWVDLAKAFG
                     LNAVVLTAEYGDVVTPERVGEALKSNPDTRGVFVQASETSTGAQHDVKSMAGLIKKTD
                     AIFVIDAITGLGTMPLDIDGWGLDIVIGGSQKAFMIPPGLAFLSASEKAWAREAQAKL
                     PRLYFNLKKERKSAANGESSWTPATALILALAEALKYVKSVGMNKLI"
     gene            830..1276
                     /locus_tag="VN788_13815"
     CDS             830..1276
                     /locus_tag="VN788_13815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HXU21656.1"
                     /translation="MPLLDRIQKDMVEAMKAKDEARLGAVRMTKAALKKHEIDSMKPL
                     DEATELQILNTLIKQRKESADLFRKGGREELALKEEQEVKVIEGYMPAAPSAEEIESA
                     IAGAIAETGVTSLKQMGVVMKSAQAKLAGKRVNGKALSDKVRAKLS"
     gene            complement(1278..1640)
                     /gene="crcB"
                     /locus_tag="VN788_13820"
     CDS             complement(1278..1640)
                     /gene="crcB"
                     /locus_tag="VN788_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HXU21657.1"
                     /translation="MQKYLMVIAGGGAGALARYVLSVAVMSWYSGRFPLGTFLINVTG
                     SFAIGLLVTVFFDSPNVRLLLVTGFLGGYTTFSSFEFESWAAFDNGARWIGLLNLVGS
                     VVFGYIAVWLGVFAAGKR"
     gene            1855..2043
                     /locus_tag="VN788_13825"
     CDS             1855..2043
                     /locus_tag="VN788_13825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXU21658.1"
                     /translation="MDSRQVIRELKRDGWYEVNQAGSHKQFKHPTKKGRVTVPYPRRD
                     IPIGTLRSIEKQAGLRFR"
     gene            2066..2503
                     /locus_tag="VN788_13830"
     CDS             2066..2503
                     /locus_tag="VN788_13830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014809903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXU21659.1"
                     /translation="MEYIAYLHKDRKSDFGVSFPDFPGCVTAGKTLEEARRMAVQALS
                     LHIEGMMEDGDAIPEPSTLDAVANDPAMKGAVAFLVNIDIAEKAERFNITARRSQIDE
                     IDRLARHRGMTRSAYMVASALSGKRSGELRELRGRRGQQAGKP"
     gene            complement(2525..2737)
                     /locus_tag="VN788_13835"
     CDS             complement(2525..2737)
                     /locus_tag="VN788_13835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21660.1"
                     /translation="MATFELEYAKEHLPELFGHAIDGTEVVIVREDGQACQLTRLAAA
                     WNKEPPVDVAEVPEDPEPEIGDLVPA"
     gene            complement(2768..>3202)
                     /gene="nth"
                     /locus_tag="VN788_13840"
     CDS             complement(2768..>3202)
                     /gene="nth"
                     /locus_tag="VN788_13840"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HXU21661.1"
                     /translation="SQAEMAQDIRSTGFFNNKSKSVIGAAKKILSDYGGKVPRNIDDL
                     LTVPGAARKTANVVLGTAFGIAAGVVVDTHVTRVSRRLDMTRESEPGKIEKDLMKAIP
                     ENKWILFSHQIIHHGRALCVARKPLCAKCPLDKLCYAKDKTV"
BASE COUNT          756 a          875 c          958 g          613 t
ORIGIN      
        1 gatcagctta ttcatgccga cgctcttcac gtatttcagc gcttcggcaa gtgccagaat
       61 cagcgcggtg gctggcgtcc agctcgattc gccgttggcg gcgctcttgc gctccttctt
      121 cagattgaaa tagagccgcg gaagcttggc ttgcgcttcg cgtgcccagg ctttctcgct
      181 cgccgacaag aacgcgaggc ccggaggaat catgaaggct ttttgcgaac cgccgatcac
      241 gatgtcgagg ccccagccgt cgatgtcgag cggcatcgtg ccgagccccg tgatcgcatc
      301 gatgacgaag atagcgtcgg tcttcttgat gaggccggcc atcgacttca cgtcgtgctg
      361 cgcgccggtg gaggtttcag atgcctgcac gaaaacgcca cgagtgtccg gattcgattt
      421 cagcgcttct ccaacgcgct ccggcgtgac cacgtcgccg tattcggcgg tgagcaccac
      481 ggcattcaaa ccgaacgcct tggccagatc cacccaacgc tcgccgaatt ttccggccga
      541 gcagacgatc actttgtcgt tcggtgaaaa gaaattggtg acggacgctt cgagcgctcc
      601 cgtgcccgac gaggtaatcg agaggacatc gttcgaagtg ccgaagactt ccttgagatc
      661 ggtcaaaaca gagcggtaga tcttccggaa atcctcagtc cggtggtgga tatcggaacc
      721 catcatcgcg tgaagagcgc tcggaagaag gggcgtcggc cccggcgtga gaagtctttg
      781 tttcctgata tgcataatga aggggtaaac aattagaata gcaaacgcga tgcctcttct
      841 cgaccgtatt caaaaagaca tggtggaagc catgaaagca aaggatgaag cgcgtttggg
      901 cgctgtccgg atgactaagg cggcgctgaa aaaacacgag atcgattcga tgaagccgct
      961 ggacgaggcc accgaattac agatattgaa tacactgatc aagcagcgga aggaatcggc
     1021 cgatctattc cgcaagggcg gacgcgaaga gcttgcgttg aaagaagaac aggaagtgaa
     1081 agtgatcgag ggctacatgc ccgccgcgcc gtccgctgaa gagatcgagt cggcgatcgc
     1141 cggcgctatc gccgaaactg gagtaacctc gcttaagcag atgggcgtcg taatgaagtc
     1201 ggctcaggcg aaactcgccg ggaagcgcgt caatgggaag gcgctgagcg acaaggtccg
     1261 cgcaaagctg agttgaatta ccgctttccg gccgcgaaca cgcctagcca aacggcgatg
     1321 tagccgaaca cgacgctacc caccaagttc agcaggccga tccaccgcgc cccgttatcg
     1381 aacgcggccc acgattcaaa ttcaaaactg gaaaatgtcg tatagccgcc aagaaagcca
     1441 gtgaccagga ggagccgtac gtttggtgaa tcgaaaaaga ccgtgacaag caggccgatg
     1501 gcgaacgagc cagtgacgtt gatgaggaac gtgcccagcg ggaaacggcc actataccaa
     1561 ctcatcacgg ccacgctgag aacgtagcgc gccaatgccc ccgcgcctcc accggcgata
     1621 accatcagat atttctgcaa tgaaaaatcc tcccggcgcg ggagcgatct tacgaggaag
     1681 aaactcccac agccatttca ctgtaggagt cattggccgc cggagcggcg ttaggcgaac
     1741 cccaccgcca aacgtgagaa tacaacagag tcgcgggcga gttcaatgag gcaggctgtt
     1801 tgacgagtgt gtagaaagtg tgtgtaatga ggataagagg atgaaggacc ggaagtggat
     1861 agccggcagg ttatcaggga gttaaagcga gacggctggt acgaagtgaa tcaggccgga
     1921 agccacaagc aattcaaaca tcccacgaag aaaggacgcg tcacggtccc gtatccaaga
     1981 agggacatcc cgattgggac cctgaggagc atcgaaaagc aggccggtct gaggtttcga
     2041 tgaggctggc gcaaacggag caggcatgga gtacatcgca tatcttcata aagatcggaa
     2101 gtccgatttc ggcgtaagct tccccgattt tcctggctgt gtcacggccg gcaagacgct
     2161 cgaggaagcc cgccgcatgg ccgtccaagc gttgagctta cacatcgaag ggatgatgga
     2221 ggacggagac gcgatcccgg agccctcaac cctggacgct gtggcgaacg atccggccat
     2281 gaagggcgcg gttgccttcc tggtgaacat cgacattgcg gaaaaggcgg agcggttcaa
     2341 cattacggcg cggaggagtc agatcgacga aatcgaccgg ctggccaggc atcgcggcat
     2401 gacgcgttcc gcctacatgg ttgcctcagc tttgagcggg aagcggagcg gcgaattgcg
     2461 cgagttgcgc ggccgacgcg gacagcaggc cggaaaaccg taagcgtttc cgagcatgac
     2521 gccgctatgc gggaaccaga tctccgattt caggttccgg atcttcggga acttcagcca
     2581 cgtccacagg cggctcttta ttccatgctg ccgccaggcg ggtaagttga cacgcttgtc
     2641 cgtcttcgcg gacaatcacc acctcggttc catcgatagc gtggccaaag agttcgggta
     2701 gatgctcttt cgcgtattca agctcgaatg tcgccataat tcgtagacgc gcacgggacg
     2761 acttccatca cacagtcttg tctttggcgt agcagagttt gtccagaggg catttcgcgc
     2821 agagcggttt ccgcgccacg cacagagccc ggccgtggtg gataatctga tgcgaaaaga
     2881 gaatccattt gttttcgggg atcgctttca tcagatcttt ttcgattttg cccggttccg
     2941 attccctggt catgtcgaga cggcgggaaa cgcgcgtgac gtgcgtgtca acgacgacgc
     3001 cggccgcgat accaaacgca gtgccgagta caacgttagc cgtcttccgt gccgcacccg
     3061 gaactgtcag cagatcgtcg atgtttcgcg ggacctttcc cccgtaatcg gaaagaatct
     3121 ttttggcagc gccgatcacg cttttcgatt tgttattgaa gaagccggtg gagcgaatat
     3181 cctgagccat ctcggcctgg ct
//
LOCUS       DATNDT010000423         2865 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1179136, whole genome shotgun sequence.
ACCESSION   DATNDT010000423 DATNDT010000000
VERSION     DATNDT010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2865
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1179136"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VN788_13845"
     CDS             complement(<1..641)
                     /locus_tag="VN788_13845"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HXU21662.1"
                     /translation="MHSRQMMKQVLVPLALAVLTIAAPALASAQQPSAGGFKFPAPSP
                     QNFTAVSPSRATVEAFLKVAWGYDANRMWQVWAIEKTPAPGVSRVIVEVAQKNDPQHQ
                     IESATFLVTPDGKHLVSTNLEMLPFGAHPYAEARETLMQRANGPSRGAASKVDEIVEF
                     ADFQCPHCKASEPIIQRLLTDFPQAHFVFENFPLTSIHTEAFKAAAYSECVAQ"
     gene            complement(721..2367)
                     /locus_tag="VN788_13850"
     CDS             complement(721..2367)
                     /locus_tag="VN788_13850"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HXU21663.1"
                     /translation="MKIALAQINPTVGDFDGNARKIIDFANRAAKGRATLVIFPELAV
                     CGYPPADLLEKPAFVARAGEVIEEIAGHTADGKTAIICGYVTPAPKGTGKHVMNSAAL
                     LRNGRVDFVQSKMLLPFYDVFDEQRYFAAAHDQRLLSIAGEQAALTICEDAWNDKSFW
                     ENRLYNVDPVEELMHAGGSLILNISASPYWRGKLKTRRDMLSAIARHHCKPVIMVNQV
                     GGNDSLVFDGSSLALGPDGEVIAQAKSFEEDLVYVDTESLTGDMHSQSQSEDEATYQA
                     LVLGTRDYVRKCGFSKVIVGLSGGIDSALVVAIAAEAVGSENVLTLGMPSPYSSQGSI
                     EDSRHLAQNLGVQFEVLPISEIFEHYNKTLAPLFAGLTPDITEENLQARIRGALLMAA
                     SNKFNSLVLTTGNKSEMSTGYSTLYGDMVGALAVIGDVFKTRVYALCRYINREREIIP
                     KAILEKPPSAELRPDQKDTDSLPPYEVLDPVLEAYVERYETPDTIAKAHNLDPVLVRR
                     IVKLVERSEYKRQQAAPVLKVTPKAFGMGRRFPIAAKIEV"
     gene            complement(2403..>2865)
                     /locus_tag="VN788_13855"
     CDS             complement(2403..>2865)
                     /locus_tag="VN788_13855"
                     /inference="COORDINATES: protein motif:HMM:NF025292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HXU21664.1"
                     /translation="MEATAADFVGTWRLRDYSFLHEDGTVEHPWGTAVRGYLLYSAEG
                     YMSGNLSPANDWPSRRARLKAALKAHDGASAFRLARRAGRRDYIAYTGRYTVEKGTVT
                     HHVEASLFPNWVGRSEVRYYRFEGNTLTLRTGPVRAGRDTIIAQLVWERVD"
BASE COUNT          559 a          808 c          884 g          614 t
ORIGIN      
        1 tgctgcgcga cgcactcgct ataggccgcg gccttgaaag cctcagtgtg aatgctggtg
       61 agcggaaaat tttcaaagac aaaatgagct tgcgggaagt cggtgagcag ccgctgaatg
      121 attggctccg aggctttgca gtgggggcac tggaaatcgg cgaactcgac gatctcgtcc
      181 actttgcttg cggctccgcg gctgggtcca ttggctcgct gcatcaatgt ctcgcgggct
      241 tccgcgtagg gatgcgcgcc gaagggcagc atctccagat tggtggagac gaggtgtttg
      301 ccatcgggcg tgacgaggaa tgtggcggat tcgatctgat gctgcggatc gtttttctgt
      361 gcgacctcca cgatcactcg actgacccca ggcgcgggcg tcttttcaat cgcccatacc
      421 tgccacatcc ggttcgcatc gtaaccccag gctactttca gaaaagcttc gaccgtggcc
      481 cgcgacggcg aaacagccgt aaagttctgc gggctgggag cgggaaattt gaagcccccg
      541 gcgcttggct gctgggccga ggcgagggcc ggcgccgcaa tggttagaac cgcgagagca
      601 agaggaacca ggacctgctt catcatttgt cgagagtgca ttgcatttcc ttaagaaaat
      661 ttgtgggaag gcccgcgatc agtagtgtaa gggttggacg ctccgccgtg gaatttcgca
      721 tcagacctca atcttagcgg cgatggggaa acggcggccc attccaaagg ccttgggcgt
      781 aactttgagc accggcgcgg cctgctggcg cttgtactcg ctgcgttcca ccagttttac
      841 gatccggcgc accagtaccg gatcaaggtt gtgggctttg gcaatggtgt ctggcgtttc
      901 atagcgctcg acataagcct cgaggacagg atcgagcacc tcatacggcg gcagcgagtc
      961 tgtgtccttc tggtccgggc gcagttcggc ggagggtggc ttttccagaa tggccttggg
     1021 gataatttcg cgctcgcggt tgatgtaacg gcacaaggca tacacccgcg tcttgaacac
     1081 gtcgccgatg acagcgagcg cgcccaccat gtctccgtag agcgtggaat agccggtgga
     1141 catttcgctt ttatttccgg tggtcagcac cagcgaattg aatttgttcg aagccgccat
     1201 gagcagcgcg ccgcgaattc gcgcctgaag gttttcttcc gttatgtctg gagtgagtcc
     1261 ggcaaagagc ggagcgagcg ttttgttata gtgttcgaat atttcgctga tgggcagaac
     1321 ctcgaattgg acgccgagat tctgtgccaa gtgacggcta tcttcgattg agccttgtga
     1381 ggaatacggg ctcggcattc ccagcgtgag gacgttttcg ctgcccaccg cctcagcggc
     1441 aatcgctacg accagcgcgg aatcgatgcc tccgctgagg ccgacaatga cttttgagaa
     1501 gccgcacttg cgcacataat cgcgcgtgcc gagaaccagc gcctgatatg tggcctcgtc
     1561 ttcggattgc gactgcgagt gcatgtctcc ggtgagcgat tccgtatcga catagaccag
     1621 gtcctcttca aaggacttcg cctgcgcaat cacttcccca tcaggcccta atgcgagact
     1681 cgatccatcg aataccagcg aatcgttgcc gcccacctgg ttgaccatga tgacgggctt
     1741 gcagtggtgg cgggcaatgg ctgaaagcat gtcgcggcgc gttttgagct tgccgcgcca
     1801 ataaggagac gcggagatgt tcaagatgag tgaaccgccg gcatgcatca gttcctccac
     1861 cggatcgacg ttataaaggc ggttttccca gaagctcttg tcgttccagg cgtcctcgca
     1921 gatggtgagc gcggcctgct cgcctgcgat gctgaggagt cgctggtcgt gtgcggctgc
     1981 aaagtagcgc tgctcgtcaa aaacatcgta gaaaggcagc agcattttgg actgcacaaa
     2041 gtccactcgc ccgttgcgca gtaatgcggc ggagttcatg acgtgcttgc cggtgccctt
     2101 aggggcgggc gtgacgtagc cacagatgat ggcagttttg ccatcagccg tgtggcccgc
     2161 aatttcttca atgacctcgc cggcgcgcgc cacgaaagcg ggcttttcca gcaaatccgc
     2221 aggaggatag ccgcagacgg caagctccgg aaagataacc agagtggctc ggcctttggc
     2281 cgcccggttg gcaaagtcga taattttgcg ggcgtttccg tcgaagtctc caacggtggg
     2341 gttgatctgg gcgagcgcga tcttcacaac gataagttta cctgctaatg gaattgcgac
     2401 ggctagtcca cgcgctccca cacaagctgc gcgatgatcg tgtctcttcc ggcgcgaacc
     2461 ggcccggtgc gtagcgtgag ggtgttgccc tcgaacctgt agtaacgaac ttccgatcgg
     2521 cctacccagt tcggaaacag actggcctca acgtgatgtg tcaccgtgcc tttctcgaca
     2581 gtgtagcggc cggtataggc gatgtagtcc cggcgtcccg cgcgacgcgc cagcctgaac
     2641 gcgctcgcgc cgtcgtgggc cttcaaagcc gccttcagcc gggcgcggcg cgagggccag
     2701 tcattcgcgg ggctcaggtt tccgctcatg taaccttcgg cggaataaag cagatagccg
     2761 cgcacggcgg tcccccaggg atgctccacc gtcccgtcct catggaggaa cgagtagtcg
     2821 cgaagacgcc atgtgcctac aaaatccgct gcggtggctt ccatc
//
LOCUS       DATNDT010000424         3688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3909618, whole genome shotgun sequence.
ACCESSION   DATNDT010000424 DATNDT010000000
VERSION     DATNDT010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3909618"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="VN788_13860"
     CDS             complement(<1..228)
                     /locus_tag="VN788_13860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21665.1"
                     /translation="MKHRVARSANAPRTSRNRLDKKGLSSAVKAPPDPDAPAKELQLK
                     LYEEALTHFQQQKFHRAQQVLERVLEGPSKEL"
     gene            complement(367..1212)
                     /locus_tag="VN788_13865"
     CDS             complement(367..1212)
                     /locus_tag="VN788_13865"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HXU21666.1"
                     /translation="MLRAEIERLFDENPARYNDEHHRLFQQFKDALNAGEVRAAEPDA
                     ASRSGWRVNAWVKKGILLGFRMGAIVDMSIDAARQPFFDKSTYPVKRFSVDSGVRIVP
                     GGSSARDGCYVGKGVTCMPPMFINAGAYVGDGSMIDSHALVGSCAQVGRKCHISAAAQ
                     IGGVLEPVGALPVIIEDEALIGGNCGIYEGTVVKSRAVLGSGTILNRSTPVYDIMTGE
                     VHSAGADDDEPLVIPANAVVVAGARALPEGRGKGWNLSLYTPVIVKYRDAKTDAKVRL
                     EDLLR"
     gene            complement(1199..2098)
                     /gene="dapA"
                     /locus_tag="VN788_13870"
     CDS             complement(1199..2098)
                     /gene="dapA"
                     /locus_tag="VN788_13870"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HXU21667.1"
                     /translation="MFTGCGTALVTPFRQDLSLDEETLRKLVQRQIDAGINFLVPCGT
                     TGESPTLNREEQLRLVEITIEEARDSKTPVVAGAGGANTAHVIEMAREFKALGASGIL
                     SVTPYYNKPTQEGLYQHYKAIAEATSLPIILYNIPGRTGVNIEPATLVRLAEIENIVA
                     VKEASGNISQMAAILDKVPQRFLVLSGDDAITLPLMALGGRGVISVSSNEIPAEMVRI
                     VQMALANDFAAAREIHRRYARLLEANFIETNPGPVKAAMALMGLLEPVWRLPMVAPKP
                     ENLAKIRSALESVGLVQQVYAAR"
     gene            complement(2122..2817)
                     /locus_tag="VN788_13875"
     CDS             complement(2122..2817)
                     /locus_tag="VN788_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HXU21668.1"
                     /translation="MNLAIVGYGKMGRLIEQLAPEYGCEVKLRLDVDNNAKFEGLTKE
                     NFRGIDAAMEFSTPTVALENIERLAGLGVNTVVGTTGWFGEIERARSAVERDGTGLVW
                     SPNFSVGVSLFNQIVSEAARLFARQPEYGAWAWEIHHATKKDSFSGTLLALVEKMKQN
                     GYEKRIDTSSSRAGAQPGTHEIGFDSSADTITIRHTARSREGFARGALRAARWIAGKK
                     GFFDFSEIVGELE"
     gene            complement(2833..>3688)
                     /locus_tag="VN788_13880"
     CDS             complement(2833..>3688)
                     /locus_tag="VN788_13880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXU21669.1"
                     /translation="NVLACWGNPVVTLSTHMDTVPPFFASREDADFIWGRGACDAKGI
                     IASMTGAVEKLLGEGARNFALLFVVGEERNSAGAAVAARAPRGSKYLVNGEPTENKLA
                     LASKGILRFEIEAHGRMAHSAYPELGESAIEALLDALDAVRRMPLPHDALLGRCTVNI
                     GTISGGRAPNVIADAAKAEILVRLVGDPAPLREAFARVAGERVQVKEILCIPAMRFQN
                     VDGMPTTVVSYTTDVPMFGTSWGTPLLLGPGSIHVAHTLEERISKRDLAEAVEMYAGV
                     VRKLLAAG"
BASE COUNT          740 a         1289 c          895 g          764 t
ORIGIN      
        1 aagttctttg ctggggcctt ccaatacgcg ttcgagtacc tgctgggcgc gatgaaattt
       61 ctgctgctga aaatgcgtta gcgcctcttc gtagagcttt agctgcagct ctttggccgg
      121 cgcgtcgggg tccgggggcg ccttcacagc agacgataag ccctttttat ccaaacggtt
      181 gcggcttgtc cggggggcat ttgcgcttcg agcaacacga tgtttcacag gtgaactcca
      241 agaaaccagg cacaaaaaca tagcaaaatc aggccagcac gtcaatcaaa cagagcaaag
      301 ccgtttcagc ggcacgttct aggctgggtg caaactcaag ccgctctgca agttacgcga
      361 ggcctgtcat cgcaggaggt cctcgagtct taccttcgca tcggtcttcg catcgcggta
      421 tttcacgatt actggcgtat agagcgaaag attccatccc tttccgcgcc cctcaggaag
      481 tgcgcgcgca cccgccacca caaccgcatt cgccggaatc accagcggct catcgtcgtc
      541 cgcgcccgcg ctgtgaacct cgcccgtcat gatgtcatac acaggggttg aacgattcag
      601 gatcgttccg ctgccgagca cggcgcgact ctttaccacc gtgccctcat agatcccgca
      661 attgccgccg atcagcgctt cgtcttcgat aatcacgggc agcgcgccga ccggctcgag
      721 cacgccgcca atctgcgccg cggccgaaat gtggcatttg cgcccaacct gcgcgcaact
      781 ccccaccaac gcgtgtgaat cgatcattga tccatcgccc acatacgcac cagcattgat
      841 aaacatcggc ggcatgcagg taacgccctt tcccacgtag caaccgtccc gcgcgctcga
      901 tcccccgggc acaattcgca cgcccgaatc caccgaaaac cgcttcaccg gatatgtgga
      961 cttatcgaag aaaggttggc gcgccgcgtc aatcgacatg tccacgatcg cgcccatcct
     1021 gaatcccagc aagatgcctt tcttcaccca cgcgttcacc cgccagccgc ttcgggacgc
     1081 cgcatcaggc tccgccgcgc gcacctctcc cgcgttcagc gcatctttga attgttggaa
     1141 cagccgatgg tgctcgtcgt tataccgcgc cggattttcg tcaaacagcc gctcgatttc
     1201 agcgcgcagc ataaacctgc tgaaccagcc ccaccgattc gagcgcgctg cgaatttttg
     1261 ccaaattctc cggtttcggc gccaccatcg gcagccgcca gaccggctcc agcagtccca
     1321 tcaatgccat cgccgctttc actgggccag gattcgtctc gatgaagttt gcttccaaca
     1381 accgcgcgta ccgtcggtga atttcgcgcg ctgcggcgaa atcattcgca agcgccatct
     1441 gtacgatcct caccatctcc gccggaattt cattggacga aaccgaaatc acgccgcgcc
     1501 cgcccagcgc catcagaggc agcgtgattg cgtcgtcgcc agacagcact aaaaatctct
     1561 gcggcacctt atccaggatc gcagccattt gcgaaatatt tccagaagct tcctttaccg
     1621 ccacaatatt ctcgatctcc gcaagccgca ccaaagtcgc cggttcgatg ttgacgcccg
     1681 tgcgtcccgg aatgttgtaa agaataatcg gcagcgaggt cgcttcggca atcgccttgt
     1741 aatgctgata aagcccttcc tgcgttggct tattgtagta gggcgtcacg gacagaattc
     1801 cgctcgcgcc gagcgcctta aattcgcgcg ccatttcgat cacatgcgcc gtgttggctc
     1861 cgccggcgcc ggctaccacc ggcgttttgg aatcgcgcgc ctcttcaatt gtgatctcta
     1921 ccaaacgcag ttgctcctcg cggttcagcg tggggctttc gcccgtcgtg ccgcaaggca
     1981 ccaaaaaatt aatccccgca tcgatttgcc gctgcaccag cttccgcagc gtctcttcat
     2041 ccagcgaaag gtcctgccga aacggagtca ccagcgcggt gccgcatccc gtaaacattt
     2101 gcccgcctcc ttccgaccac attactccag ttcgcctaca atctcgctaa agtcgaaaaa
     2161 tccttttttg ccggcaatcc accgcgccgc tcgcagcgcc cctcgcgcaa aaccttcccg
     2221 gctgcgcgcc gtatggcgga tggttatggt atcggccgac gaatcgaatc cgatttcgtg
     2281 cgttccaggc tgcgcgccgg cgcggctgct gctcgtatcg attcgcttct cgtagccatt
     2341 ctgtttcatt ttttccacca gcgcgagcag cgtacccgaa aacgagtcct ttttcgtggc
     2401 gtggtggatt tcccacgccc acgcgccgta ttccggctgg cgcgcgaaca gccgcgccgc
     2461 ctcgctcacg atctgattga acaggctgac tcccaccgaa aaattgggac tccaaaccaa
     2521 ccccgtcccg tcgcgttcca cagccgatcg cgcccgctca atttcgccga accatcccgt
     2581 cgttccaacc acggtattca ctcccagccc cgccagccgt tcaatgtttt ccagcgccac
     2641 tgtcggagtc gaaaattcca tggccgcatc gattccccga aaattctctt tcgtgagccc
     2701 ctcgaatttc gcgttgttat ccacatccag ccgcagcttc acctcgcacc catattccgg
     2761 agcgagttgc tcgatcagcc gccccatttt cccgtacccg acaatcgcga ggttcatcaa
     2821 gaattcctga gcttacccgg ccgccaataa ttttcttacg acgccggcat acatctccac
     2881 agcttccgcc aaatcgcgct tcgatatccg ctcctccagc gtgtgcgcca cgtgaatgct
     2941 ccccggcccc aacagcaggg gcgtgcccca gctcgttccg aacatcggca cgtccgtcgt
     3001 gtaggataca accgtcgtcg gcattccatc gacgttttga aatcgcatcg ccggaatgca
     3061 aagaatttcc ttcacctgca cacgctcccc agccacgcgc gcgaaagcct cccgcagcgg
     3121 tgccggatcg cctacaagtc gcaccagaat ttcagctttg gccgcgtccg caatcacgtt
     3181 gggcgcgcgc ccacccgaaa tcgttccgat attcaccgtg caacggccaa gcagcgcatc
     3241 gtgcggcagc ggcatccgcc tcaccgcatc gagcgcatcc agcagcgcct caatagccga
     3301 ttcgcccaat tcaggatagg ccgagtgcgc catccgcccg tgcgcctcaa tctcaaagcg
     3361 caaaatccct ttcgatgcga gcgccagctt gttctccgta ggctcgccgt tcaccagata
     3421 cttcgaaccg cgaggtgccc gcgccgccac cgcagctccc gcgctgttgc gttcctctcc
     3481 caccacaaat agcagggcga agttccgagc gccctcaccc agcagttttt ccaccgcccc
     3541 ggtcatcgac gcgatgatgc ctttcgcatc gcacgcgccg cgtccccaaa taaaatccgc
     3601 atcctcgcgt gacgcaaaaa acggcggcac ggtgtccatg tgcgtcgaaa gcgtcacaac
     3661 tggatttccc cagcacgcca acacgttg
//
LOCUS       DATNDT010000425         6521 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2679124, whole genome shotgun sequence.
ACCESSION   DATNDT010000425 DATNDT010000000
VERSION     DATNDT010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2679124"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..904)
                     /locus_tag="VN788_13885"
     CDS             complement(<1..904)
                     /locus_tag="VN788_13885"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008243870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HXU21670.1"
                     /translation="MNSRKQFSLTKQLSLMLTAAMLSPGLALAQHDLSGAAKTKLALE
                     RLQVTGSLLMIGAHPDDENTALLAYFARGRKVRTGYLSLTRGEGGQNLIGSEQGDALG
                     VIRTQELLAARRIDGAEQFFTRAIDFGFTKTPQETFQKWGRDKILSDVVWVIRRFRPD
                     VVVLRFTGTPRDGHGQHQASAILGKEAYSAAADPNRFPEQLRWVQPWQAKRLMWNVFS
                     FSREQEKEAEKLPNRVMVDPGEYDPLLGYSYAEIAGMSRSMHRSQGMGAPQRLGSEKQ
                     YLTTIAGDPAKSDVFDGVDTTWNRV"
     gene            983..2398
                     /locus_tag="VN788_13890"
     CDS             983..2398
                     /locus_tag="VN788_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU21671.1"
                     /translation="MNETVSSPQTRVLIADDEESQRLGLQSMIQSWGFAVETAADGQE
                     ALEKVSALPIHVLVTDLMMPRMDGFELLKRLSTQGNMPPAIVLTAFGNIETAIQTIHD
                     LGAFWFLEKPIQPAALKILLDRAAQQSRLTEETERLQRELQYKGVLVDMVGACPPMQQ
                     VFSLIRQVAPSRAAVFVTGESGTGKELVARAIHHLSPRRNGPFVAINCAAMPETLMES
                     ELFGHEKGAFTGALERRAGCFELAQHGTLLLDELGEMPVGTQAKLLRVLEDSRVRRLG
                     GKNEIAVDVRIIASTNKVIEEALKKGELREDLYYRLNVFQIALPPLRQREEDIPLLTD
                     ALIGVLDRKHGCRVTEVSSDVMDMFRKFSWPGNIRELRNVLERAVIVAGEGAITAHHL
                     PLDFGASLGAKPAQVFEPDGVRLPVGTTVAEGEKALIHLTLQHTKNNKTRAAEILGIS
                     LKTLFNKLKEYGAAADVEAGA"
     gene            2399..4270
                     /locus_tag="VN788_13895"
     CDS             2399..4270
                     /locus_tag="VN788_13895"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21672.1"
                     /translation="MSLKSRLRVSIVALVALVVIALSALYLYDFTGEAFTNASDSARL
                     TAEEVKGYLLERIDENQPSLPAPGAPFEEQKAAWYRIMSQDALFHRMLRDSLGSANMV
                     SNIWVTDDKGIVLAASEPLLVGSPAPPVTNFDQWERGHRLLSLWELFHSSRDYGFPLS
                     IGDDKNLKKVVTITVAVQSLFLRHQIRTPLGDLLLALTVSLLVAIALALSLPHLVLRP
                     LERVSERIDLIRAGQFSSLPPASPGESREFAAVQSKLNLLGQQFRGAKEDALGLRSNV
                     DQLLQRLEETVLLFDGRRRLVMAGKGLEEMLGQRGADLAGADLNEVFPPDSPLGRIVC
                     TAVDGHAPVRDRVVVLDRNGRGTSRLLVNVEMVEQSDGSRQIGTLVTLRDAESRRQLA
                     SHLDFSSRLAAIGRLTSGVAHEIKNPLNAMALHLEVLRNKLEEPAPEINVIAGEIKRL
                     DRVIKTFLNFNRPLDLAIQEINMSDLLTNISSLIEPDARARGVSVKTLLTHSLWINGD
                     PNLLKQAILNVVVNGLEAMTDGGLLTLSSERRGKDCLVTIADTGPGIPPAVQDKIFQL
                     YFTTKTQGSGIGLAMTFRMVQLHSGTIDFESEPGLGTSFRIRLPEITSVVARNGIVT"
     gene            4567..4959
                     /locus_tag="VN788_13900"
     CDS             4567..4959
                     /locus_tag="VN788_13900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21673.1"
                     /translation="MRRAKNTVPPQGSAQQVTVAPTQSGEPENSTPEQPSLDTQEPAP
                     RLGPLLSAADRQRYESEIDGFLSNAGKNLSSLTEHTLSKDQQALKLQAENFIKQAKEL
                     RSSDLPAARSLAQRADLLASHLAENFRQ"
     gene            complement(5038..5805)
                     /locus_tag="VN788_13905"
     CDS             complement(5038..5805)
                     /locus_tag="VN788_13905"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXU21674.1"
                     /translation="MKAIVATPIVLPGVLALLLATGGCATKKHVAQAIAPVQNQVNTV
                     QKQTDENKTAIGDLDRQVASADEKAMDAGRKAGDAATAASKANDAASQAGQRADSAHT
                     LAEQTGAKLDQTVQNMDNYRLANTEKVLFRFGRADLTKDAKASLDSMAQNVSTMKNYL
                     LEVEGYTDRTGGKNYNLALSQRRADAVVRYLTLHNVPLRKIHQIGVGSEALNADNHTR
                     EARKQERRVDVRVYALDTTGGNGPLQSSTSPAATTQQ"
     gene            6052..>6521
                     /locus_tag="VN788_13910"
     CDS             6052..>6521
                     /locus_tag="VN788_13910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HXU21675.1"
                     /translation="MTQTAALRATPLHRVHRESGAKMVDFGGWEMPIQYSSILTEHNA
                     VRRAVGLFDVSHMGEIEVRGPEALRLVDFVSTNAAHKLKQGQAHYSGLLYEHGGFVDD
                     ILVHKLADDHFFLCVNAANQEKDFEHIRSLNRWDAEVEFASDRYAQLAIQGPRSL"
BASE COUNT         1358 a         1872 c         1877 g         1414 t
ORIGIN      
        1 gcacgcggtt ccaggttgta tccacgccat cgaagacatc gctcttggcc ggatcgcccg
       61 caatcgttgt gaggtactgt ttttcggagc caagccgctg aggcgctccc attccctggc
      121 tgcggtgcat gctgcggctc atgccggcga tctcggcgta agaatagccg agcagcgggt
      181 cgtattcgcc ggggtccacc atgacgcgat tgggcagctt ttcggcttcc ttctcttgct
      241 cgcgtgagaa gctgaacaca ttccacatca gccgtttggc ctgccagggt tgtacccatc
      301 gcaattgttc cgggaagcga ttcggatcag cggcggcgga gtaggcttcc ttcccgagaa
      361 tcgccgaagc ctggtgctgt ccatgcccat cgcgcggcgt cccggtaaat cgcaggacga
      421 cgacatccgg gcggaaccgg cggatcaccc acacgacatc ggacaggatt ttatcgcggc
      481 cccatttctg aaacgtctct tgtggcgttt tggtgaagcc gaagtcgatc gcgcgggtga
      541 agaattgctc cgcgccatca atgcggcggg cggccagaag ctcctgcgtg cgaattaccc
      601 cgagagcgtc gccctgttcg gagccaatca gattttggcc tccttcgccg cgcgtcaagg
      661 aaagatagcc ggtgcgcacc ttgcgcccgc gggcgaaata cgcgagtaaa gcggtgttct
      721 cgtcgtcggg gtgcgcgcca atcatcagca ggctgcccgt cacctggaga cgctccagcg
      781 ccagcttggt tttagcggcg cccgagagat cgtgctgagc cagcgcgagc cccggactga
      841 gcatcgcggc ggttaacatc aaggaaagtt gctttgtgag agagaactgt ttgcgcgaat
      901 tcacggaacc tactaggtga tcacgggcga ggtttggatg caagcgtctc attttgcggt
      961 gcttcagtta caatacgtgt gaatgaacga aacggtctcc agtccgcaaa cccgcgtcct
     1021 gattgccgat gacgaagaga gccagcggct tggactgcaa agcatgatcc agtcgtgggg
     1081 cttcgcggtc gaaaccgcgg ccgatggcca ggaagccctc gaaaaagtga gcgcgttgcc
     1141 gattcatgtt ctggtaaccg atctcatgat gccgcgaatg gatggcttcg aattgcttaa
     1201 acgtctatcg acacagggca acatgccgcc agctattgtt ttgacggctt tcggaaacat
     1261 cgaaacggcg attcagacga ttcacgatct tggcgcattc tggttcctcg aaaagccgat
     1321 ccagccggcc gctctcaaaa ttcttctcga tcgcgccgcc caacagagca gacttacgga
     1381 ggagaccgag cgtctccagc gggaactgca atacaaaggt gtgctggtgg atatggtggg
     1441 cgcgtgtccg ccgatgcagc aggtattttc gctgatccgc caagtggcgc cttcgcgcgc
     1501 cgccgttttc gttacgggcg agagcggtac gggcaaagag cttgtcgccc gcgctattca
     1561 tcacctcagc ccgcgccgca atgggccatt cgtggccatc aattgcgccg caatgcccga
     1621 aacgctgatg gagagcgagc tgttcggcca tgaaaaagga gctttcactg gggctttgga
     1681 acggcgcgcc ggttgcttcg agcttgcgca gcacgggacg ctgctgctcg atgagttggg
     1741 tgagatgccc gtgggcacac aggcgaaact ccttcgcgtc ctcgaagatt cccgcgtacg
     1801 ccggctcggc ggaaaaaacg aaattgccgt ggacgtgaga atcatcgcct ccaccaataa
     1861 agtgatcgaa gaagcgctta agaaaggcga gcttcgcgag gatctctatt atcgcctgaa
     1921 cgtttttcag attgctctgc ctcctctgcg ccagagggag gaagatattc cgctgctgac
     1981 cgacgcttta atcggcgttc tcgaccggaa gcatgggtgc agagtcacgg aagtatcgtc
     2041 cgatgtaatg gatatgtttc gcaaattttc ctggccgggc aatatccggg agttgcggaa
     2101 cgtgctggag cgcgccgtta tcgtcgctgg agaaggagcg atcacggcgc atcatcttcc
     2161 gctcgatttt ggcgcctccc tgggagcgaa gccggcgcag gtgttcgagc cggacggcgt
     2221 caggctgccg gtgggcacga cagtcgcgga aggagaaaaa gcgttgatcc acctcaccct
     2281 gcagcatacg aagaacaaca agacccgcgc ggccgaaatc cttgggatca gcttgaagac
     2341 gctttttaac aagctcaagg aatacggcgc cgcggccgac gtcgaagctg gcgcgtaaat
     2401 gtccctgaag tcacggctgc gagtctcgat agtggcgctg gtcgctctcg tggtaatcgc
     2461 gctgtcggct ttgtacttat acgatttcac gggcgaagcg tttacgaacg cttccgatag
     2521 cgcccgcctc accgccgaag aggtgaaggg ctatctgctg gagcgtatcg acgagaatca
     2581 gccttctctt cccgcgcctg gcgctccttt cgaggaacag aaagccgcgt ggtaccggat
     2641 catgagccag gacgcgttgt tccacagaat gctgcgcgac agcctgggga gcgcaaacat
     2701 ggtctcgaac atctgggtca ccgacgacaa aggtatcgtt ttggccgcct ctgaaccgct
     2761 tttggtaggt tccccggcgc ccccagtcac gaatttcgac cagtgggaac gcgggcaccg
     2821 gctgttgagt ctctgggagt tgtttcacag cagccgcgat tacggttttc ccctctcgat
     2881 cggcgacgac aagaatctca aaaaagtggt gacgatcacc gtggcggtcc agtcgctctt
     2941 tctacggcat cagatccgga cgcctctcgg agatctcctc ctggctctca ctgtttcgct
     3001 gctggtggca atcgccctgg cgctctcgct accgcatctg gttctacgcc cgctcgaacg
     3061 cgtcagcgag cggatcgatc tgattcgggc ggggcaattc tccagccttc ccccggcctc
     3121 gccgggggag tctcgtgagt tcgcggccgt gcaatcgaaa ttgaatcttc tcggccagca
     3181 gttccgcggc gcgaaagaag acgctctcgg gcttcgttcg aacgtcgatc agttattaca
     3241 gcgtctggag gaaacggttc tactgttcga tggccgccgc cggttagtga tggcgggcaa
     3301 agggctcgag gagatgctgg gccaacgcgg cgcggatttg gccggcgccg atctgaacga
     3361 agttttcccg cccgattcgc cgctgggacg cattgtttgc actgccgtgg atggccatgc
     3421 cccggtgcgg gatcgcgtcg tagttctcga cagaaatgga cgcggcacat cccgtcttct
     3481 cgtaaatgtc gagatggtgg agcagagcga cggctcgcgc cagattggga ctctggtcac
     3541 tctccgggat gctgaatcgc ggcgccagct tgcatctcac ctcgatttct catcgcgttt
     3601 ggccgccatc ggccggctca ccagcggcgt cgctcatgag atcaagaacc cgctgaacgc
     3661 aatggctctg catctcgaag ttctccggaa taagttggaa gaaccggcgc ctgagatcaa
     3721 cgtgattgcc ggcgagatca agcggctcga tcgggtgatt aagacttttc tcaatttcaa
     3781 tcgcccactg gatctggcta ttcaagagat caatatgtcg gacctactga cgaatatctc
     3841 ttctctgatt gagccggacg cgagggcgcg gggcgtctca gtcaagacat tgcttacgca
     3901 ctccttatgg atcaatgggg atccaaacct tctgaagcag gcaattctga atgtagtggt
     3961 caatggcttg gaagccatga cagatggagg tttgctaacg ctcagttctg aacgccgtgg
     4021 caaagactgc ctggttacga ttgccgacac cgggccgggc attccacccg ccgttcagga
     4081 taaaattttc caattatatt tcacgacgaa aacacaaggc tccggcattg gcctggctat
     4141 gacttttcgt atggtccaac ttcacagtgg tacaatcgac ttcgaaagcg aaccggggct
     4201 tgggacctcg ttccggattc gtcttccgga aattaccagt gtcgtggcca gaaacgggat
     4261 agtaacttaa aggaccagcc ggaaacgggt tctctctcaa tgacacagcg gtttccggcg
     4321 tgctcagtta cgctggcggt aattttctcg ctttcaacct ggtcgtgctc caaaaagcac
     4381 gcgaccatta agctcccccc gccctcttcc acacagacgg cccccgagac caaatccact
     4441 cccgttacgc cgcctccgcc agagttgcca gcgtccacga cgcaagccag cttgggcccg
     4501 tccctaccgg tgaatatgca ggttcctcca ccacctccca aaaacaagaa gaggaaacgc
     4561 aatcacgtgc ggcgcgcaaa gaacacagtg ccgccgcagg gatccgcaca gcaggtaact
     4621 gtggctccaa cgcaatccgg cgaacccgag aactccactc cggagcagcc atccctcgat
     4681 acgcaagaac cggccccgcg cttaggtcct ctgctttccg ccgcggaccg ccagcggtat
     4741 gagtctgaga tcgatggatt tctttccaat gccggaaaga atctctcttc gctcactgaa
     4801 cataccttga gcaaggatca gcaggcgctg aaactccaag ccgagaactt cattaaacaa
     4861 gctaaagaac tgcgatcgtc ggacttgccg gcggcaagga gtttggcaca acgcgcggat
     4921 ttactggcaa gccacctcgc ggagaatttc aggcagtgaa tatccaaaaa aagaggagct
     4981 tagaaccgag tggttccgcg ctcctcttgc taggaaatcg acccgcaatc tctacttcta
     5041 ctgctgtgtt gttgccgctg gagacgtaga cgactgaagc gggccattcc cgccggtagt
     5101 atccagtgcg tataccctaa cgtccacgcg gcgctcttgc ttccgagctt cgcgcgtgtg
     5161 attgtcggcg ttcagcgctt cgctgcctac cccgatctgg tgaatcttgc gcagcggaac
     5221 attatgtaaa gtcaggtacc gaaccaccgc gtcggcccgc ctttgactca gggcgagatt
     5281 gtagttcttg ccgccagtgc gatcggtgta tccttccacc tcaagcagat aattcttcat
     5341 ggtggatacg ttctgcgcca tggaatcaag cgaagccttg gcatctttcg tcagatccgc
     5401 ccgcccaaag cggaaaagga ccttttcggt gttggcaaga cgataattgt ccatgttttg
     5461 gacagtttgg tcgagcttgg ctcccgtctg ttccgccagc gtgtgggcac tatcggcgcg
     5521 ctggccagct tgggatgcgg catcatttgc tttgctcgca gcagtggcag cgtcccccgc
     5581 cttacggccg gcatccattg ccttctcatc ggcgctggca acctggcggt caaggtctcc
     5641 aattgccgtt ttgttttcgt ccgtctgttt ctgaaccgtg ttgacctggt tctgcactgg
     5701 cgcaatcgct tgtgcaacgt gcttcttggt cgcgcatcca ccggttgcca acaataaggc
     5761 tagaactccg ggaagtacga ttggtgtggc gacaatagcc ttcataagag acacactcct
     5821 cgctaaaagc actgcaaccc gatcaccagt attgaaaatc aaagcgtttc acggcttctg
     5881 ccgccggttt gccggaaacc agcgtttcac ggggcaagta gaatttacaa aattcacacg
     5941 tataatatgg gaaaccttac cggggcaagt gatatctatc gcaacacgcg cgcagccaga
     6001 tccccggctt tctgtagccg tgaaagtccg tactgctaga atgaggtttt catgacccag
     6061 accgccgcac ttcgcgcgac cccgttgcat cgagttcatc gtgagtcggg cgcgaaaatg
     6121 gtcgatttcg gcggctggga aatgcccatc caatactcca gcatccttac ggagcataat
     6181 gctgtccgcc gcgccgtcgg gctgttcgat gtgagccata tgggcgagat agaggtgcgt
     6241 ggtccggaag cgctcaggtt ggtcgatttt gtttccacaa acgccgcgca taagttgaag
     6301 caggggcaag ctcattactc ggggcttcta tacgagcatg gcgggtttgt cgacgacata
     6361 ctggttcaca agctggcgga cgatcatttc ttcctatgcg taaacgcggc gaaccaggag
     6421 aaggatttcg agcatatccg gtcgttaaat cgctgggacg cggaggtgga gtttgcgagc
     6481 gaccggtacg cgcagcttgc cattcaagga ccgagatctc t
//
LOCUS       DATNDT010000426        19469 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1682261, whole genome shotgun sequence.
ACCESSION   DATNDT010000426 DATNDT010000000
VERSION     DATNDT010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 19469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19469
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1682261"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1337
                     /locus_tag="VN788_13915"
     CDS             <1..1337
                     /locus_tag="VN788_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21676.1"
                     /translation="AALAGESIACEISWKDRRFESHLQPLRDVDGFVQGVIGVALDIT
                     ERERLTDQLRQSQKMQAIGELAGGVAHDFNNLLMVVKGHSQLLLDRLPDLSPLRSGAQ
                     QIEKAADRAASLTRQLLAFSRKQVLQPRVLDMNEVVNGMIRMFSRVIGENIDMAFVPA
                     SDLARVRADPGQIEQVLLNLVVNARDAMPTGGRLTIETSNVELDRSYAASHASVEPGD
                     YVMLSVTDTGCGMNEETQSRIFEPFFTTKGPGKGTGLGLATVYGVVKQSGGYIYVYSE
                     IDRGTTFKIYLPQVIAKLDKLAGETVAPGEARGTETILFVEDEQSVRELVRQYLTSTG
                     YSVLEAADGVEALKVAAKHSGQIHLVITDVVMPHLSGPDLATQLGASRAGLKVLFISG
                     YTDDTVFRHGVLEGGVAFLQKPFNLKALAAKIRELLEGEPVGAMTGVESEKV"
     gene            complement(1379..1648)
                     /locus_tag="VN788_13920"
     CDS             complement(1379..1648)
                     /locus_tag="VN788_13920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013578952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21677.1"
                     /translation="MDEKRFYDEKEETKQIPMTCPHCRQESTFPVRWMVRTKKAQPPG
                     GSNEEDRKQFAKARSYMVRIDNMVACRNIRCRKRFDLSGQTVFLI"
     gene            1780..2070
                     /locus_tag="VN788_13925"
     CDS             1780..2070
                     /locus_tag="VN788_13925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21678.1"
                     /translation="MAAIPIHHAMIAIMQKDNVASAHATQSLGYALGWLRLPISRPSR
                     PHYHSSIAPFSDDAGKQWAPKAKRRTHPPRLFARRGLDCIIAAIQLFGNSCR"
     gene            2498..3142
                     /locus_tag="VN788_13930"
     CDS             2498..3142
                     /locus_tag="VN788_13930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21679.1"
                     /translation="MPLPSTPKTKFPTWFRWAALLWLAFWIPVYWRTWGASNFLHLCD
                     IALILTCLGIWFNSSLLISSQAVASLLVDAAWALDVGSKLFLSHPPVPGTESFFDPHY
                     PLWIRLLSLYHLVMPPLLLWAVYRLGYDRRGWLLQSAIAFPAFVAARFTSPADNINFA
                     FIDPFLHKQLGPVPVHLILSWLFMVVVAYLPVHVLLKRSFRPSINGENVGDPSG"
     gene            complement(3145..4986)
                     /locus_tag="VN788_13935"
     CDS             complement(3145..4986)
                     /locus_tag="VN788_13935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21680.1"
                     /translation="MTFRTKLFVLFMLALLLSVGTVAAGAILLTRRMLDDSNRRHSEA
                     LLGQFQRQFERRGQDVAHQVQAIADAEATVRMAIELNRPQADVSVYVNDARGVSQAHQ
                     LDFLDFVSNDGSIISSNEWPSHFGAKMDWLVQPQDWAARGPFLAKVNTQDGPALGLMS
                     VSTVRVGDKDLYIFGGARMGRDFLSSLVLPSGMRALLYLNLDSSFQAENLIGESGPVP
                     QGERLAAPIEQQRQHPGRQAFRIAWTPDTASAETFQAMPLLGRQGEVLGILLVGSSQR
                     QTSAVERRLLLLAVGVGGAGLLFGLLLSWWGAAVVTSPVNKLETWAHDVSEGKLNTRT
                     NVHGSKEIGRLVLAFNRMTAKLSEQRDRLAQTERTTSWREVAGRLADELSNPLLALRS
                     AAENIQRGQEQDPDRSNEASRALAAEMLSEVERVETIVAKFRDFASLPQPELSAVDLN
                     EIVRGIVRGYEREFGAVGKPPITPELHLEENLSLIQADAALLRRVLDNLILNAMDAMP
                     GGGVLMLKTTGEKEGVELEVSDTGTGLTPRECSRLFTPYYTTKPHGTGLGLAIVQCVV
                     SDHGGSISVESEAGVGTSFHVRLLAKPPQRPIDIPITLPVQESVPSA"
     gene            complement(4983..5438)
                     /locus_tag="VN788_13940"
     CDS             complement(4983..5438)
                     /locus_tag="VN788_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21681.1"
                     /translation="MTIHQRHPQIVLADCVNMLSIFDGSGSRGSGGFSLRTAGVLNTL
                     ASGGQSTGEYQKSSRWSKVPIHRIRAGIRGAGPLAGTPGAFYRIRCRGLGILPCRKSL
                     RDVRNKAPRAQISGSLKVTLRYDAGEPLRKIEMRGTLIFGKAAAEGTLG"
     gene            5427..7556
                     /locus_tag="VN788_13945"
     CDS             5427..7556
                     /locus_tag="VN788_13945"
                     /inference="COORDINATES: protein motif:HMM:NF024907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HXU21682.1"
                     /translation="MDRHQFAEALGRFQTACVMNPASDIGCLNMGIAFLNMRQFEDAQ
                     RVLAKSAERDPKSLRAWFSLGLLEIATGNPQGAAPDFEKVAAIDPDDADAQYFIGYLA
                     LQRQQYDVAVTAFQHAIAVDPFHPSAEQGLADAEQHLGDADGAKVHQERAQKIISAHL
                     GKPIRLIYGDEGKYSLAQEMTERAEAAPPGIPVHFTNVSATAGLPSHAETSSRASRPS
                     TRAKHQTNRANDYENAPKTLANFLGSGACIFDYDGDGRPDIFLINADGTGHPALYRLI
                     GKGKFENVTKTARLDFQVEGMGCAIGDYDHDGHPDLAVTTGGGIMLFHNEGNGEFKDV
                     TEEAGLRPSQASGAIALGVSFVDYDGDGDLDLYVTRFNDFPLAHPAQPFSFDEGAAPP
                     GNVLWRNLGDGHFVDSTKDLALEGTAASVGALGIDLNNNATIDFLLTAWQKFPSVLLN
                     GLNGKFHDTNPWAISMPGPAAGVAALDFDGDGWMDLAFTHWALPGLSVWHNIDGKSFE
                     RLPLVGPDWMRGWGIAALDYDNDGWVDLVAVGETFSGEGRIALLRNEGAAGFRDVTHE
                     TALDKVVLDNPRSVIAFDYDGDCAPDLLITQNNLPPVLLKNIGGNKNSCLKIAAVGDT
                     DNSMGIGTKVEIFSGARRQTWEISGSTGYLSQGPPEILAGLGTQSLADVVKLHWPSGL
                     LQDEIQIPAGVRISIAETDHAKNTQPQ"
     gene            complement(7604..8515)
                     /locus_tag="VN788_13950"
     CDS             complement(7604..8515)
                     /locus_tag="VN788_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXU21683.1"
                     /translation="MELGQLRYFCAVVDAGGFSRAAQRIHVSQPSLSQGIRKLEDELG
                     ARLFDRLGRTVRLTELGRAFLPRARGVLRELESARGEVQERKASVGGAICVGVIPTIA
                     PYFLPPCLAAFSRKHPEACVTVVEEITPLLVERLRAGAMDVAIVALPLPVRSHEFEIV
                     TLLKESLYAVLPERHRLVHRGKLAIEDLRDEPFLLLRDGHCFRETAIAACKRAHLNPQ
                     IIFESGQFSSILSMVSAGFGVSIIPEMALEKRAGCRFVPLSDDRAARTIGAVTLKGKS
                     TTRLQQTFLDHLRAQSPPIAVPSSSSN"
     gene            8742..9488
                     /locus_tag="VN788_13955"
     CDS             8742..9488
                     /locus_tag="VN788_13955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU21684.1"
                     /translation="MALAMRQGHPSAADLGELAPPMMELDYAKKPADYFTVERQEMLP
                     FLPPNCRRLLDVGCGAGAFGEMVKRKKKVEVWGVEPVSSVAAEASTKLDHVIVGTFSP
                     QTELPEESFDCIVFNDVLEHMLAPEQALRYATSLLSCTGSIVASIPNIRYLPILWRLA
                     VRGGWKYEDCGVLDRTHVRFFTRSSIIAMFQSEGYVVSDISGINPYQGIPQASKRLWN
                     AYEFMNALFFRKFNDLKFQQFAVVAQPTLP"
     gene            9502..10698
                     /locus_tag="VN788_13960"
     CDS             9502..10698
                     /locus_tag="VN788_13960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 99-like
                     domain-containing protein"
                     /protein_id="HXU21685.1"
                     /translation="MSTQARLIALYLPQFHPIPENDRWWGKGFTEWTNVTKAKPLFKG
                     HYQPRLPADLGYYDLRLPEVREAQAEMARDAGIAGFCYYHYWFGGKELLAQPFNEVVA
                     SGKPDFPFCLCWANQTWTGVWYGAPRRTLIEQTYLGREGHRRHFLALRKAFLDERYLR
                     VNDRPIFVIFRPNEIPDVASFIDQWQELALRNGLPGLHFVAHLFHAETPWDFRSAGFD
                     ACTVVNHLKGFSVPFRKLVLQKARHVGNGRLTKETVLHFLWRRYRSYVGRFGGHVRRY
                     QDVLPFLLDGCSAPDAYPCLIPNWDNTPRSQTGGYVLHESTPELFRIHLRGALNLIQS
                     RDPEHRLIFLKSWNEWAEGNYLEPDQKFGRDYLNVIRQELGDPGMDLEEDQRERASVG
                     LWRNHA"
     gene            10824..12302
                     /locus_tag="VN788_13965"
     CDS             10824..12302
                     /locus_tag="VN788_13965"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXU21686.1"
                     /translation="MATSKRAIFHNIVYAGLTKGTKLVCITLTSSIVARNLSPWDYGV
                     IGFAAIIIGFLNHFSDIGIGSAAVRRASLTPPSLHTAFTLKIMLGCVAFLSAYLVAPF
                     AHLFFQHPATGNVLRVLAFGFLINTLGFMPLVTLMREQNFRALMIPGMLSAIARCILV
                     IVLIALGWSYWAVVLADVGATLVSVTAIQFVRKIPFAVSFDRADAREYLRFGLPLLAS
                     GVLVFLIFNLDNLLVGSAMGSAQLGYYALAFTWGSFICGVLYDSVNSVLLPAFAEIQH
                     DLAAMRRGYLKILGFAGFVSVVANTALLANAHYFLTTFLGKGTDKWVPATVALEIFCF
                     YGILRAVTEPIGSCIMALGQTKPLLHAALLSGGIEVLLLVLALRTRKIESVAAVVLLA
                     YTIQVVVYLPFLRRNLSVTVRDIAGQLWPLIPTIAGAWLATSLLPASLGNTLITLAFR
                     ASFTALVAALVHGLCTRFRFFQEATGIIMKSVGASPGLAPRV"
     gene            complement(12532..15522)
                     /locus_tag="VN788_13970"
     CDS             complement(12532..15522)
                     /locus_tag="VN788_13970"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXU21687.1"
                     /translation="MIGETISHYRILEKLGGGGMGVVYKAEDTRLHRFVALKFLPKEV
                     ARDSQALARFLREAQAASALNHPNICTIYDIGEQEEDAYIVMEFLDGFTLKHHIAGQP
                     LELETVLSLGTEIADALDAAHAEGIVHRDIKPANIFVTKRGHAKILDFGLAKVKYGER
                     SALPVGASSAATTAITEDNLTSPGTALGTVAYMSPEQVRGKELDLRTDLFSFGVVLYE
                     MVTGALPYRGETSGVIFEAILNRAPVPPVRLNPSLPPKFEDIINRALEKDRDLRYQHA
                     SEMKSELMRLKRDTDSGRAAVVHGEEASVTPILEKKGSAESRAASAEGLAARASSPSD
                     AGSGDMRTPAPSASSAQAVGESRRRKFGAAAIAIAALVLLAVAIGGFFFFHSHAAKLT
                     DKDTVVLADFTNTTGDSVFDGTLRQGLSAQLEQSPFLSLLSDERIAQTLSLMAQPKDA
                     RLTRELARDLCQRTASAATIEGSISSLGSQYVLDLKAVSCHGGDLLAEEQATAGGKEQ
                     VLTALGGAATKMRQRLGESLSSVQKYDAPPESVTTPSLEALHAYTLGYQRTMKDDPQG
                     AVELFQRAVSLDPNFAMAYARLGTTYSNLGQVSLAAESTGKAYELRDRVSEWEKLYIA
                     SHYENFVSLDLEASRKTYELWTQTYPRDFIPHQNLSVTYGALGDYDQALHHAREALNL
                     NREAGAYEVLIEAYLGLNRLDEARATAQEARGRNLDSPGIHMSMYQLNFVQHDMAAAD
                     REISSLRGKSFFDGVALVFQSDTARYNGQFAKARRLSRRGADSLGADTKETAAAVQAE
                     SAMQEALVGNMSLAKQEANAALRLSGGRDASWVSAIALALAGDSGQAARLAGDLEKRF
                     PEDTLMQTQYLPMIRAAAALTAGKPENAIQALARAAPYELGQNRASMTLGPIYFRGQA
                     YVAAKQGASAAAEFQKIVDHPGAVENEPIGALAHLGLGRSYALSGDKAKAAAAYQDFL
                     ALWKDADPDIPILKEAKAEYAKLQ"
     gene            complement(15819..16166)
                     /locus_tag="VN788_13975"
     CDS             complement(15819..16166)
                     /locus_tag="VN788_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HXU21688.1"
                     /translation="MPHYLIQAAYTSEAWQAMVKNPQSRIEAVRPAIERLGGKLVGGW
                     AAFGDFDTILIAELPTSVDAAAIAIAAAAGGSCRAVKTTPLLTAEEAVEAAKKASTTG
                     YKPVAASASAAKR"
     gene            16338..18707
                     /locus_tag="VN788_13980"
     CDS             16338..18707
                     /locus_tag="VN788_13980"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU21689.1"
                     /translation="MSALNSINSNLDMSPFEPHRAKDAREPYFWRVEGSLLNLTAVRP
                     VAFFTWNAQTFTERWARRGLMGMMAVTRPLLYAADRVFATRVMHAVLRGESRDRLDLL
                     GEEFFHYFLKPHLKPRGVEKLREFTESGADVVLVSQGLDHIMRPLANYLGVRRILSNR
                     LDFREGIATGRLLDPVIRPRGPFAKLRSDDRPDGRVVREKLLWDLGFGRKPDTLDATI
                     RPAARPSQRVSLPVVHFDSRDKNAPLSVRRALRGKQILLIGATGFIGKVWLANLLTEL
                     PEIGRVYLLIRGHRTATALERFERIVSESPVFEKLAARHGDRFAGFLRERIEVISGDA
                     SEPGLGLPIEDRQRLTRSLDLIVNSSGLTDFNPDLRDALKMNVRAISYLLDFLRGCDH
                     AGLLHLSTCYAVGQRDGRIVEDLPKDYIPIGLRDFDAQKEWHALENLVRQVEARANSV
                     EVTDELRRQAQEKQHAAKELHGAALDNQIRKNRVRWLRQALVDAGMKRANELGWPNTY
                     TFTKSLSESLIRNFLDASPHAAIAVVRPSIVESSVEKPFLGWNEGVNTSASLSYLLGT
                     FFRQLPTNASKCLDLIPVDLVCRGMILISAALVERRHERMYQLATSVANPCDMRRSIE
                     LTGLGHRKYYRSQNGFQHRLRSKFDAIPVSKARYEKLSAPAQKIIVQAINRTVEPIPF
                     VRPPLARQERELEKVIKLIALFEPFILHNDHVFEAINVESLSAALPGDERAAFGYDAR
                     SIDWWDYWINVHIPALRKWCYPLIEGRQPENGPRRNVGFISRTATEIAD"
     gene            18664..>19469
                     /locus_tag="VN788_13985"
     CDS             18664..>19469
                     /locus_tag="VN788_13985"
                     /inference="COORDINATES: protein motif:HMM:NF019605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU21690.1"
                     /translation="MSASSAALPQRSRTNRAIPGNDSLTTENVPIFLTGSTGYIGAHV
                     ATNLLVAQGQTLNLLVRARDSQEVRGRLWHALQLHMDLPQFEELVDSRVHIFLGDLTA
                     EHFGLAGDDYHKLVRSTDSVIHCAASLNRKSEKSCLNVNLRGTLEVIQLAARSRDDHG
                     LRRFSHVSTVAVAGHRANEVVQEDEAIDWNRSDYDPYARTKKFCEHMLRQLLADVPRT
                     IFRPSIVLGDSRRPETNQFDMVRAFVFLAGLPALPFRATDKVDIVPVDYVA"
BASE COUNT         3991 a         5886 c         5333 g         4259 t
ORIGIN      
        1 gtgccgccct tgccggcgag tccattgcct gcgaaatttc ctggaaggac cggcgctttg
       61 aatcgcatct tcagcctctc cgcgatgtcg acgggtttgt tcagggcgtc ataggggtgg
      121 cgctggatat cacagagcgc gagcgactca cggaccagtt acgccaatcg cagaagatgc
      181 aagctattgg tgagctcgcg ggaggagtcg cccatgactt caacaatttg ctcatggttg
      241 tgaaagggca ttcccagctc ctccttgacc gcctgcccga tctctcgccc ctgcggtcgg
      301 gagcgcagca aatcgaaaaa gcggcggacc gcgctgcatc gctcacccgg caattgctgg
      361 cctttagccg caaacaagtt ctccaacccc gtgtcctaga catgaatgaa gtggtcaacg
      421 gaatgattcg catgttctcc cgcgtaatcg gagagaacat cgacatggcc tttgtccctg
      481 ccagcgatct cgcgcgcgta agagccgatc ccggacaaat cgagcaggtc ctgctgaatc
      541 tggtggtcaa cgcgcgggat gctatgccta ccggcggccg cctaacgatc gaaacgtcca
      601 atgtcgaact cgatcgtagc tatgccgcat ctcacgccag cgtcgaaccc ggcgattatg
      661 tgatgctgag cgtcaccgat accggctgcg gcatgaacga ggaaacgcaa tctcgcatct
      721 ttgagccatt ttttacaacc aagggaccgg gtaagggcac cggcctcggt cttgcgacgg
      781 tctacggggt tgtgaagcag agcggcggtt acatctacgt ctacagcgaa atcgatcgcg
      841 gcacgacttt caaaatttac ctgccccaag tcattgcgaa actcgacaag ctcgcgggcg
      901 aaacggtcgc tccaggcgag gcacggggta cggaaaccat cctttttgtc gaagacgagc
      961 agagcgtgcg cgagctcgtg cgacagtact tgacttcaac cggctactct gtgcttgagg
     1021 cagcggacgg agtggaggcg ctcaaggttg ccgccaaaca ttctgggcaa atacatttag
     1081 tgatcacaga cgtagtgatg cctcatctta gtggtcccga cctggcaacg cagttgggag
     1141 cctcgcgcgc cggcctcaag gtactattca tctccggtta caccgacgac accgtcttcc
     1201 gccacggcgt gctcgagggc ggcgttgcgt ttcttcaaaa gcccttcaac ctcaaagcac
     1261 tcgcagccaa gattcgggaa ctgctggagg gagagccggt tggcgcgatg accggtgtgg
     1321 agtccgaaaa agtttaaggg tatccgatgg atttcttgta atttctttcc ttcgctcgct
     1381 atatcaagaa aacggtctgc ccgctgaggt caaaccgctt ccggcaccgg atgttccggc
     1441 acgcaaccat attgtcaatg cgtaccatat aggatcgcgc tttggcgaat tgctttctgt
     1501 cttcctcatt tgacccgccg ggaggctgtg ccttcttggt tcgaaccatc cagcggaccg
     1561 gaaaagtgct ctcttgccgg cagtgcggac acgtcatcgg tatttgcttc gtttcttcct
     1621 tctcgtcgta aaaacgcttc tcgtccattt gcccccgcgc cagcaattct gccgcttcta
     1681 tcgcgctcgc tcaagaccct tgtgtattcc gtccagggtc acaaatataa atccggcatc
     1741 cttccacctc gggagccaat attccaaggc agcgaccgta tggctgcgat tcccattcac
     1801 cacgcgatga tcgccatcat gcaaaaggac aatgtcgcct ccgcgcacgc tacgcaatcg
     1861 ctgggttacg cgctcggctg gctgcggctt ccaatctcgc gcccatcgcg accacattac
     1921 cactcttcga tcgccccgtt ttccgacgac gctggcaagc agtgggctcc gaaagccaaa
     1981 cggcggacgc atccaccgcg gcttttcgcc cgccgcggcc tcgattgcat catcgcagcg
     2041 atccagctct tcggcaattc ttgccggtga gagaaacgtt agcgccgcgt gactgttggt
     2101 atggttccca atcgtatgcc cgctagacgc aatctctcgt gcgagtgatg gctctgcgcc
     2161 cacccatttc ccgattaaaa agaatgtacc tcgtaccccg ttacgttcca gtagctccaa
     2221 cagccccggg gtgactgccg gattcgggcc atcgtcaaac gtgagtgcga tcgtggcatc
     2281 gcttcccgtt ctccgaattg tggcaccgaa caattctgaa gagggagcaa cagcggccca
     2341 tgcaaatata ccgcccactg cgcaaactcc gccgccggcc gctaacgctg gaattccgtg
     2401 catggcttcg cgaattctct caccgctgag tacaaaacgc taccgaatgg gattcgctcc
     2461 cggcgtttgg taccattcgc gcaacatcga gccgcgaatg cccctccctt cgacgccaaa
     2521 aacaaaattt cccacctggt tccgttgggc cgcgctgtta tggctcgcat tctggattcc
     2581 ggtatattgg cggacatggg gcgcgtcgaa ttttctgcac ctgtgcgaca tcgcattgat
     2641 tttgacctgt ctcggcattt ggtttaacag ctccctcttg atttcaagcc aggcagtcgc
     2701 atcgctcctc gtggatgcag catgggcgct ggatgtggga tcgaaacttt ttctctccca
     2761 tcccccggtt ccggggaccg agagcttttt cgatcctcac tacccgctat ggatacgact
     2821 cctttcgctg taccacctcg tgatgccgcc attgctgctt tgggccgtat atcgcctcgg
     2881 atacgaccgc cgcggctggc tattgcaatc ggcgatcgca ttcccggcat tcgtcgccgc
     2941 acgatttact tcgccggctg acaacatcaa cttcgccttc atcgatccat ttcttcataa
     3001 gcagcttggc cctgtgccgg ttcaccttat tttgagctgg ttattcatgg ttgtagtcgc
     3061 gtatctccct gtacatgtcc tgctgaaacg cagcttccgc ccgtccatca acggcgaaaa
     3121 cgttggcgat ccttccggat aaaatcaagc gcttggaacg ctctcttgga ccgggagagt
     3181 aattggaatg tcaattgggc gttggggtgg ctttgccagc aggcggacgt gaaacgacgt
     3241 gcccacgccc gcttcacttt ctaccgaaat gcttccgcca tgatcgctga caacacactg
     3301 cacaatcgcc aggcccagcc cggttccgtg cggcttcgtc gtgtaatagg gagtgaacag
     3361 gcggctgcat tcgcgcggcg tcagccccgt ccccgtatcg gagacctcga gttccacacc
     3421 ttccttttcg ccggtggttt tgagcattag aacgccgccg cccggcattg cgtccatcgc
     3481 attcaatatc aagttatcga gcacgcggcg caggagagcg gcatccgcct gaatcagaga
     3541 taggttctct tccagatgca gttcgggcgt gatcggcggc ttcccgacag ctccaaattc
     3601 tcgttcgtat cctctgacga ttccgcggac aatttcgttc aaatccacag ctgacagttc
     3661 cggctgtggc aacgacgcaa aatcacggaa tttcgcgacg atcgtctcga ccctctcgac
     3721 ttccgagagc atttcggccg ccagggcacg agacgcctcg ttcgaccggt caggatcctg
     3781 ttcttgaccg cgctggatat tttcagcggc acttcgaagc gccagcagcg gattacttaa
     3841 ctcgtcggcg agtctccccg caacctcccg ccacgaagta gtgcgctccg tctgcgccaa
     3901 gcggtcccgt tgctcgctta actttgcggt catacgattg aaagccagca caagccggcc
     3961 aatttctttg ctcccgtgaa cgtttgtgcg cgtgttcaac ttcccttctg atacgtcgtg
     4021 cgcccaagtc tccagcttat ttacgggact cgtcacaact gccgcgcccc accagctaag
     4081 aagcagccca aacagtaagc ccgccccgcc aacccctact gccagcaaaa ggagccgccg
     4141 ctctaccgcg gacgtttggc gctgtgagct acccaccagc aggatgccga ggacctcgcc
     4201 ctggcggcca agcagcggca tggcctgaaa agtttccgca ctcgcagtat ccggggtcca
     4261 agcgatgcga aacgcttgcc tacccggatg ctgccgctgc tgctcgatcg gagccgcaag
     4321 ccgttcgccc tgcggcacgg gcccggactc ccctatcaaa ttttccgcct ggaagctgga
     4381 atcgagattg agatacagga gcgcgcgcat gccgctgggc aaaaccaagg aggaaaggaa
     4441 gtctcttccc atccgcgcgc cgccaaaaat gtagagatcc ttgtctccca cccgcacagt
     4501 cgaaacggac atcaacccga gcgcaggacc atcctgcgta ttcactttcg caaggaacgg
     4561 gccccgcgcc gcccaatctt gcggctgcac tagccaatcc attttggcgc cgaagtgcga
     4621 aggccactcg ttggacgaaa taatcgagcc gtcattggag acaaaatcca ggaaatcgag
     4681 ttggtgggcc tgcgaaacac cgcgcgcatc attcacgtac acggaaacat ccgcttgcgg
     4741 gcggttcagc tcgatcgcca tccggacggt ggcctccgcg tctgcgatcg cctgcacttg
     4801 gtgagccacg tcttgcccgc gccgctcgaa ctggcgttgg aattggccga gcagcgcctc
     4861 gctatgccgt cgattcgagt catccagcat gcggcgagta agaaggatcg cacctgcagc
     4921 gaccgtcccc actgaaagca ggagcgccaa cataaaaagc acgaacagct tggtgcgaaa
     4981 tgtcacccca acgttccctc agccgcagct ttaccgaaga tcagcgttcc tcgcatttcg
     5041 attttcctta gtggttcgcc cgcatcatat cgcaacgtaa ccttcagcga acctgagatt
     5101 tgcgcccttg gcgccttgtt gcgaacatcg cgaagggatt tgcggcacgg caagattcct
     5161 aatccgcggc agcgaatacg ataaaatgcg ccgggcgttc ctgcgagcgg tccggcgccg
     5221 cggatgccag cgcgaatccg atgaatcggt accttcgacc agcgactgct tttctgatac
     5281 tcgcctgtgg attgtccacc ggaggccaac gtattaagca cccccgccgt tcgcagcgaa
     5341 aatccgccgc tgccgcggct cccgcttccg tcgaaaatac tcaacatgtt gacgcaatcc
     5401 gccagaacaa tttggggatg gcgttgatgg atcgtcatca gtttgcagaa gcgcttggaa
     5461 gattccaaac ggcgtgcgtg atgaatccgg cctccgacat cggctgcctg aacatgggga
     5521 tcgctttttt gaacatgcgg cagttcgaag acgcccaaag agtactcgcg aaatccgccg
     5581 agcgcgatcc gaaaagtttg cgcgcctggt tcagtctggg cttactcgaa atcgcgacag
     5641 gcaacccgca gggggccgct ccggatttcg aaaaagttgc tgcgattgat ccggacgatg
     5701 ccgatgcgca gtactttatc ggatacttag cgttgcagcg gcaacagtac gacgtagccg
     5761 tcaccgcctt tcagcatgcc attgctgtcg atcctttcca cccctccgcc gagcagggtt
     5821 tggccgatgc ggagcagcac ctcggcgacg ctgatggcgc gaaagtgcat caggaacgcg
     5881 cgcagaaaat catttcggcg cacctcggca aacccattcg tctcatatac ggcgacgagg
     5941 gcaaatattc gctggcgcag gaaatgactg agcgggcaga agccgcgccg cccggaattc
     6001 ccgttcattt caccaacgtc agtgcgaccg ccggcctgcc atcccacgcg gaaacgagct
     6061 cgcgagctag ccgccccagc acacgcgcca agcaccaaac caatcgcgcg aacgattacg
     6121 agaacgcacc gaaaacactc gcgaatttcc tcggcagcgg tgcctgcata ttcgattacg
     6181 acggagatgg ccgcccggat atttttctga tcaatgcgga tggcacgggc catccagcgc
     6241 tttatcgcct tatcggtaaa ggaaaatttg agaatgtcac gaaaacggcg cggctcgatt
     6301 ttcaggttga aggaatgggc tgcgccatcg gcgactatga ccatgacgga catcccgatc
     6361 tcgccgtcac caccggtggc ggaatcatgc tctttcacaa cgaaggaaac ggtgagttca
     6421 aggacgtcac cgaagaagct ggcctccgcc cgagccaagc gtccggcgcg atcgccctcg
     6481 gtgtgtcgtt cgttgactac gatggcgatg gcgatctcga tctttatgtc acccgattca
     6541 acgatttccc tctcgctcat cccgcccagc cattttcgtt tgacgaaggc gctgcgccac
     6601 caggaaacgt cctctggcgt aacctaggcg acggccattt tgtcgattca acgaaagatc
     6661 ttgcactcga gggcaccgca gcatctgttg gcgcgctcgg aatcgacctc aacaacaacg
     6721 ccacgatcga tttcctatta accgcttggc agaagtttcc atcggtgctt ctaaacgggc
     6781 tgaacggcaa atttcacgac acaaatccct gggctatcag catgccgggg cctgcagccg
     6841 gtgtggcggc ccttgatttc gatggcgacg gctggatgga cttggccttc acccactggg
     6901 cgctaccagg cctcagcgtt tggcacaaca ttgatggaaa gtccttcgag cgccttccgc
     6961 tcgtcggccc ggactggatg cgcggatggg gcatcgcagc gctcgactat gacaatgacg
     7021 gctgggtcga cttggtcgcc gtcggcgaaa cgttctccgg cgaaggtcgc atcgcgctgc
     7081 ttcgcaatga aggtgcggca ggctttcgcg atgtcacgca cgaaaccgcg ctggacaaag
     7141 tcgtgctaga caacccacga agcgtgatcg cctttgacta cgatggcgac tgcgcaccgg
     7201 atctgctaat cacgcagaat aacctgccgc ccgtcctgct taagaacata ggaggcaata
     7261 agaacagctg cttaaagatt gccgccgtcg gcgatacgga taactcgatg ggcatcggca
     7321 caaaagtgga aattttctcc ggcgcaaggc gccagacttg ggagatctcc gggtcaacgg
     7381 gatacctcag ccaaggacct ccggagattc tcgccggcct cggcacgcag agcttggccg
     7441 acgtggtcaa actgcattgg ccctccggat tgctacagga cgaaatccaa attcccgccg
     7501 gcgtccgcat ctccatcgcg gaaaccgacc acgccaaaaa cactcagccc cagtgacggc
     7561 aagcgccttc ttttgcgtgg ggtttccttt gatcttgtgg caattagttc gaagaggatg
     7621 agggcactgc aatcggaggg gactgtgcgc gcaagtgatc cagaaaggtt tgttgcagcc
     7681 gagtggtgga cttccctttc agcgtcacgg cgccgatggt gcgggccgcc cgatcatcgc
     7741 tcagcgggac aaatcggcag cccgcccttt tctccagggc catttccggg atgatggaaa
     7801 cgccgaaccc ggcgcttacc atgctcagaa tgctgctgaa ctggccgctc tcgaaaataa
     7861 tctgcggatt caagtgggcg cgtttacatg cggcgatcgc cgtttcgcga aagcagtggc
     7921 catcgcgcag cagcaggaag ggctcgtcgc gaaggtcctc aatcgccagc ttgccgcggt
     7981 ggaccaatcg atgccgctcg ggcagcaccg cgtaaaggct ttccttgagg agcgtcacga
     8041 tttcaaattc gtggctacgc acaggcagag gcagcgcgac gatcgccacg tccatcgccc
     8101 ccgcgcgaag ccgttcgacc agcagcggag taatctcctc gaccacagtg acgcaagcct
     8161 cgggatgttt gcgagaaaac gccgcaaggc acggcggaag aaaataagga gcgatagtcg
     8221 gaatcacgcc tacgcaaatc gcgccaccga ccgaagcttt acgctcttga acttctcctc
     8281 gggccgactc aagctcccgc aacacgccgc gagcgcgtgg caggaaggcg cgcccaagct
     8341 cggtcagccg caccgtgcgc cccagacgat cgaatagcct cgcgccaagc tcatcttcaa
     8401 gctttcgaat tccctgcgaa agcgaaggct gcgagacatg gatgcgctgt gcggcgcggc
     8461 tgaatccccc ggcgtccacc accgcgcaaa agtagcgaag ctgcccgagt tccataggat
     8521 caacctatct gtgagctaag aaactatatg ctggaagcat gacaaaaagt caacgctacg
     8581 gcggcgcggg aatttgttag cgaggcgcgt cgccgctcac gggtggtcac ttcactcaca
     8641 ataatgagtc tccttaagta cttgtcgccg cagctcagta cagacatcag tgtattgccc
     8701 gtccgattgc ccgcggttga agattggccc gagtgatgtt tatggcgctg gcaatgagac
     8761 aaggccatcc gagcgcggcg gatttggggg agcttgctcc tcctatgatg gagctagatt
     8821 acgcaaagaa gccagcggat tactttaccg tcgaacgcca ggaaatgttg ccctttttac
     8881 ccccgaattg caggcgtttg ctggacgtcg gctgtggcgc cggggcattt ggtgagatgg
     8941 taaagcggaa gaagaaagtt gaggtctggg gggtcgagcc tgtcagttct gtagcggcag
     9001 aggcatcaac caagcttgac catgtcattg tcgggacctt ctccccacaa actgaactgc
     9061 ctgaggaaag cttcgattgc atcgtattta acgacgtgct cgagcatatg ttggcgccgg
     9121 agcaagctct ccgttatgcc acgtctcttc tgtcctgcac cggttcaatt gtggcttcca
     9181 ttcctaatat tcggtatctg ccgatccttt ggcggttggc ggtccgcggt ggatggaagt
     9241 acgaagattg cggcgtattg gacagaactc atgtccgatt ttttacgaga tcgagcatta
     9301 tcgcgatgtt ccagagcgaa ggttacgtcg tgagtgatat ttcgggaatt aatccctacc
     9361 agggaattcc tcaggcgagt aaacgcttgt ggaacgcgta tgagtttatg aatgcgcttt
     9421 tcttcagaaa gttcaacgac ctgaagttcc agcaatttgc agtcgtcgcc cagccaaccc
     9481 taccttaaac ggccgaggcc tatgagcaca caagcccgat tgattgcatt gtatcttcca
     9541 cagtttcacc cgatccccga gaatgaccgt tggtggggca agggattcac cgagtggacc
     9601 aacgtcacaa aagccaaacc gcttttcaaa ggacactatc agccccgttt acccgcggac
     9661 ctgggttact acgatctccg gttgccggag gtgagggagg cacaagcaga aatggcccgc
     9721 gatgcgggca ttgccggatt ctgctattac cactattggt ttggtgggaa agaactgctt
     9781 gcgcaaccat tcaatgaggt ggttgcttca ggaaaaccgg acttcccgtt ttgtctctgc
     9841 tgggccaatc aaacctggac cggcgtgtgg tatggcgctc cccgccgtac tctcattgaa
     9901 caaacctatc taggacggga aggccatcgc cggcattttc tagcgctgcg caaggcgttc
     9961 ctggacgaac gctatctgcg agtgaatgat cggccgattt tcgtaatctt tcgacccaat
    10021 gagattccgg atgtagccag ctttatcgac caatggcagg aactcgcact tcggaatggc
    10081 ttgcccggct tgcactttgt ggctcatttg ttccatgcag agacgccttg ggatttccgg
    10141 tcagcggggt tcgacgcgtg cactgtagtt aatcacctca aggggttcag cgtgccattt
    10201 cgcaaactgg ttctgcaaaa agcccggcac gtgggaaatg ggcgcctaac caaagagacc
    10261 gttcttcact ttctttggcg acggtaccgc tcctatgtgg gcagattcgg cggccatgtg
    10321 aggcgatatc aggatgtcct tccatttctg ctcgacggtt gcagcgcacc tgacgcgtat
    10381 ccctgcctga taccaaactg ggacaataca ccgagatctc aaacaggagg atacgtgctg
    10441 cacgaatcga cgccggaatt atttcgcata cacctgaggg gtgccctaaa tttgatccag
    10501 tcgcgcgacc cagaacaccg gctgattttt ctaaagtcat ggaatgaatg ggcggaagga
    10561 aattacttgg agcctgatca gaaattcgga cgcgattatc tcaatgtcat taggcaagaa
    10621 ctcggggatc cgggaatgga cctggaagaa gaccagcggg agcgagcgtc cgtgggtctt
    10681 tggagaaatc acgcctagcc atcgtgtaca tctccttccc ggggataccg aaacgtgctc
    10741 gatttaaggg tttggctgga tgatctcgct agccattcca gcagcacatt ggattcggtc
    10801 cgatcgtcct ttgccgcaaa cccatggcca cttccaaacg cgcgattttc cataacatcg
    10861 tttatgccgg cctaaccaag gggacgaaac tggtctgtat aacgctcacc agttccattg
    10921 tcgccagaaa tctctcgccc tgggattacg gggttattgg gtttgcagcc atcataatcg
    10981 gttttctaaa ccattttagt gacatcggga tcggtagcgc tgctgttcgc cgcgcttcgc
    11041 tcactccgcc aagtcttcac actgcgttta ctctgaagat catgctcggc tgcgtggcgt
    11101 ttcttagcgc ttacctcgtc gccccgttcg cgcacctgtt tttccagcat cccgcaacgg
    11161 gcaacgtgct tcgggtcctg gccttcggtt tcctgatcaa caccctcgga ttcatgcccc
    11221 tggtgacgct aatgcgggag cagaatttcc gggcgctgat gatcccaggg atgttgagcg
    11281 cgatcgcccg ctgtattctc gttatcgtgc tgatcgcgct cggatggagc tactgggcag
    11341 ttgtgctcgc cgacgtgggt gcaacactag tcagtgtgac agccattcaa ttcgtgagaa
    11401 aaattccctt cgccgtaagt tttgaccggg cggatgcccg cgaatatctg cgatttggcc
    11461 tgccgctgct cgcaagcggc gttctcgttt ttctcatctt taatctggat aacctcctgg
    11521 tcggctccgc catgggaagc gcccaactcg gatactatgc gctggcgttt acgtggggaa
    11581 gtttcatctg tggtgttctc tatgattcgg tgaacagcgt attgctcccg gcatttgccg
    11641 aaattcaaca cgatttggcc gcgatgaggc gagggtacct gaagatcctg ggtttcgccg
    11701 gcttcgtctc cgtggttgcc aatactgcgt tgcttgccaa cgcccactat tttcttacaa
    11761 catttttggg taagggcact gataagtggg tgcccgcgac cgtcgcgctt gagattttct
    11821 gcttctatgg aatcctacgg gctgtcaccg agccgatcgg gagctgtatc atggccttgg
    11881 ggcaaaccaa gcctcttttg catgccgctc ttctctcggg cggcatcgaa gtattgttgc
    11941 tggttctggc gttgcgcacc agaaagattg aatccgttgc ggccgtcgtg ctcttggcat
    12001 atacgatcca agtggtcgta tatttgccct ttctgcgtcg aaatctcagc gtaaccgtcc
    12061 gggatattgc tgggcagcta tggcctttga ttccaaccat agctggagcc tggctggcaa
    12121 cctcgctcct gcccgcctcg ctcggcaaca ctttgatcac cctcgccttc cgtgcgtcat
    12181 tcaccgcttt ggtagccgcc ctagtgcacg ggctctgtac ccgcttccgc ttcttccagg
    12241 aggccacagg catcatcatg aaaagtgtag gcgcgagtcc tgggctagcg ccccgcgtat
    12301 agcgccatcg ggaagtggca tcagcagagc aatacttcct ccaagctggt ttaggcccct
    12361 aagtccacaa tcaccgtgga tgatttttct ttacaccaac aagtacttgc gcgatttcga
    12421 ccccctgtgt aaagattttc ctcgtatcct cggactacgc cttaccgcca attcgcgacc
    12481 cctgattacg atttgcctca gcgcttcggt tggaacgtgt gtgcaccgca cttactgaag
    12541 ctttgcgtac tcggctttgg cttccttcag gattgggatg tcagggtcag catctttcca
    12601 aagcgcgaga aaatcctggt aagcggctgc cgccttcgct ttatcgccag aaagcgcgta
    12661 ggagcggcca agtccaaggt gcgccagcgc gccgatcggt tcgttttcaa cggcgccggg
    12721 gtgatcgaca attttctgga actcggctgc cgcggatgcg ccttgcttcg cggccacata
    12781 cgcctgtccg cgaaaataaa tcggacctaa agtcatgcta gctcggttct gtccgagctc
    12841 gtacggcgcg gcccgcgcaa gggcttgaat cgcgttttca ggctttcccg cggtcagagc
    12901 agcggcagcg cgaatcatcg gcagatattg ggtttgcatg agcgtgtctt ccggaaaacg
    12961 cttttccaag tcgccggcaa gccgtgcagc ctgcccggag tcacccgcca gcgccagcgc
    13021 aattgccgaa acccacgacg cgtctcttcc accggagagt cgcagtgccg cgttcgcttc
    13081 ctgcttcgct aagctcatat tgcccaccag ggcctcttgc atcgcgcttt ccgcctgcac
    13141 tgcggccgcc gtctccttcg tgtcagcgcc cagggagtcg gcgccgcgcc gcgagagtct
    13201 gcgggccttc gcgaattgtc cgttataccg ggctgtgtcc gattgaaaaa cgagcgcgac
    13261 tccatcgaaa aacgacttcc ctctcaggct cgaaatttcg cggtcggcgg cggccatgtc
    13321 gtgttgcaca aaattcagtt ggtacatcga catgtgaatt cctggggagt cgaggttacg
    13381 gccccgagcc tcctgcgcag tggccctggc ttcgtccaaa cgattgagac ctaaatatgc
    13441 ttcgatcagc acttcgtaag caccggcctc gcgattcaga ttgagtgcct cccgagcgtg
    13501 atgcaacgcc tgatcgtagt ctccaagcgc tccatatgtg acgctcaggt tctgatgcgg
    13561 gatgaaatcg cgcgggtacg tctgggtcca tagctcgtag gttttgcgcg aagcttccag
    13621 atcaagactc acaaaattct cgtagtgtga tgcgatgtag agtttctccc actcgcttac
    13681 ccggtcgcga agctcgtagg ctttccctgt actttccgcc gccaagctaa cttgacccag
    13741 attactgtac gtcgttccca gccgcgcgta agccatggca aagttcggat cgagactgac
    13801 ggcgcgctgg aacaactcaa ccgccccctg gggatcgtcc ttcattgttc gctggtagcc
    13861 gagggtgtac gcgtgcaacg cctcgagcga cggggtggta acactctcgg gcggcgcatc
    13921 gtacttttgc acggaactaa gcgactcacc cagcctctgg cgcatcttcg tggctgcgcc
    13981 gccgagcgcc gtcagcactt gctcttttcc gccagccgtg gcctgctctt ccgcgagtaa
    14041 atcgccacca tggcagctca cagccttcaa atcgagcacg tactggctgc ccaagcttga
    14101 aatcgaccct tcgattgtcg cggcgctcgc agtgcgctgg caaagatcgc gcgccaactc
    14161 gcgagtcagc cgcgcatctt tcggctgcgc catcaacgaa agcgtttgcg caatgcgctc
    14221 gtcggaaagc agactcaaaa atggcgactg ctccagttgc gccgaaagcc cctgtcgcaa
    14281 cgtaccgtca aaaacagaat cgccggtggt gttggtaaag tcagcaagca ccacagtgtc
    14341 tttgtcggta agcttcgccg catgcgaatg gaagaaaaag aaaccgccaa tcgcaacggc
    14401 aagcaggaca agagctgcaa tcgcaatggc agcggcgccg aacttccgcc gtctcgactc
    14461 gccgaccgcc tgcgcagagc ttgccgaagg cgcgggtgtc cgcatatcgc ctgagccggc
    14521 atcgcttggc gatgaagctc tcgccgccaa accctctgcc gaagcagccc gcgattctgc
    14581 cgagcctttc ttttccagaa tcggcgtgac gctcgcctct tcgccgtgta ccaccgccgc
    14641 ccggcccgaa tccgtatcgc gcttcaatcg catcagttcc gacttcatct ccgaagcgtg
    14701 ctggtagcgc agatcgcggt ctttttccag cgcgcgattg atgatgtctt cgaacttggg
    14761 aggcaaactc ggattcagcc tcactggagg aaccggcgcg cgattcaaaa tcgcctcgaa
    14821 aatcactccc gatgtttcgc ccctgtaagg cagtgcgccg gtcaccattt catacagcac
    14881 cacaccaaac gaaaacagat cggtgcgcaa atccagttct ttccctcgca cttgctccgg
    14941 cgacatatag gccacggtgc ccagcgcggt tccagggctg gtcaggttat cttccgtaat
    15001 cgcagtcgtc gcggctgacg atgcgcccac cggcagcgcc gaacgttcgc cgtatttcac
    15061 ctttgccaac ccaaaatcga ggatcttcgc gtgcccgcgc ttggtcacaa aaatgttggc
    15121 cggcttgata tcccgatgca cgatgccttc agcgtgggcg gcgtcgagcg catctgcgat
    15181 ttcagttcct agagaaagca ctgtttcgag ttcaagcggc tgccccgcga tgtgatgctt
    15241 tagcgtgaat ccatccaaaa actccatcac gatgtaggcg tcttcttcct gttcgccgat
    15301 gtcgtaaatc gtgcagatat tgggatgatt cagtgcggat gcggcctggg cctcgcggag
    15361 aaatcgcgcc agcgcctgtg agtcgcgggc tacttctttt ggcaggaact tgagggcgac
    15421 gaatcgatga agccgcgtgt cttcggcctt atagaccacg cccattccgc caccgccaag
    15481 cttctccaga atgcggtagt gggaaatcgt ttcaccgatc atgtgaagcg cacgtaacct
    15541 cgcgtaatgt taggggtaga ggcaaagcga agtcaaagcc ttcatcgctg ccgccggtat
    15601 gtctcgacga cccgcggagt tttgcaccgt gctcatctgt gcctcgcgcc taatcgacac
    15661 ttgagccgag taggatttga cggaactcat agaaagtaaa ggccgggcgc acaccctacc
    15721 cgtcacgtcc tccgcgaacc ccaatcgaga atcggcttag gaagtacgcg gcgatgctcg
    15781 agaaagatag cttctctgtt cgttgcgccg gcgcgtggct atcgcttagc agcagacgcg
    15841 gatgccgcaa cgggtttata cccagtcgtg gacgcttttt tggccgcttc cacggcttct
    15901 tcagcggtga gcagcggcgt ggtcttgacg gctctgcagc taccgcccgc cgccgcagca
    15961 atggcgattg ccgcggcatc cacactggta ggcagctctg cgatcagtat cgtatcgaag
    16021 tctccaaatg ccgcccagcc tccaaccagc tttccgccca agcgttcgat tgctgggcga
    16081 acggcttcga tgcggctctg cggattctta accatcgcct gccaagcttc cgaagtgtac
    16141 gcagcttgta taaggtagtg gggcatggag ccctcctttg cggtgaaatt tgcggccact
    16201 ctgacagacg agcgcgtcga agtcaacgcg gtcccgggac tcgctgaaag catctgatgg
    16261 gacccccgcg agcttgattg ggccgctgta agcatgataa ttttggcaag tggcagctgt
    16321 tgactttcca gccgaccatg tccgctttga attccataaa ctcaaatctg gatatgtccc
    16381 ctttcgagcc gcatcgagca aaagatgcgc gcgagccgta tttttggcgc gtcgagggca
    16441 gtctcctcaa tttgacggct gtccggcccg tagccttctt cacttggaac gctcagacgt
    16501 tcactgagcg ctgggcgcgg cgcggcctga tgggcatgat ggccgtcacg cggccgctgc
    16561 tctacgccgc cgaccgcgtg tttgccaccc gcgtaatgca tgcagttctg cgaggtgaaa
    16621 gccgcgaccg gctggatctg ctcggcgagg aatttttcca ctattttttg aagccccacc
    16681 tgaagcctcg aggcgtggaa aagctgcggg aattcactga atccggagcg gatgtagtgc
    16741 tggtcagcca gggactggat cacatcatgc gtcctctggc gaattacctc ggcgttcgcc
    16801 gcattctctc caaccgcctc gattttcggg aaggcatcgc cacgggacgt ttgctcgatc
    16861 ccgtgattcg gccgcgcggc cccttcgcca aactccgatc cgacgatcgc ccggacggcc
    16921 gggtagttcg tgaaaaatta ctgtgggatc ttggctttgg aagaaagccc gacactctgg
    16981 acgcaaccat acgcccggcg gcgcgccctt cacaacgcgt cagcctgccc gtggtccact
    17041 tcgactctcg cgacaaaaat gcaccccttt ccgtgcggcg cgccttgcgc ggaaaacaga
    17101 ttttgctgat cggcgcaacc ggattcatag gaaaagtgtg gctcgcgaat ttgttgacgg
    17161 aacttccgga gattggccgc gtttacctgc tgattcgcgg acaccgcacc gccacggcgc
    17221 tcgagcgttt cgagcgcatt gtctccgagt cgcccgtatt cgaaaagctg gcggcgcgcc
    17281 acggcgaccg cttcgccggc ttccttcgcg aacgcatcga agtaatcagc ggagacgcaa
    17341 gcgaaccagg ccttgggctt ccaattgaag atcgccagcg cctcacgcgt tcgctcgacc
    17401 tgattgtgaa cagctcgggc ctcaccgact tcaatcccga cctccgcgac gcgctgaaaa
    17461 tgaatgtccg tgcaatttcc tacctgctgg attttttgcg cggctgcgat cacgcgggcc
    17521 tgcttcatct ttccacctgc tacgccgtcg gacagcgtga cggccgaatc gtcgaggatt
    17581 taccgaagga ttacattccc atcgggctgc gcgatttcga cgcgcaaaag gagtggcacg
    17641 cgctcgaaaa tctagtccgc caggtcgagg cgcgcgcgaa ttccgtcgaa gtgaccgacg
    17701 aactgcgtcg ccaggctcaa gaaaagcagc acgccgccaa ggaacttcac ggtgccgcct
    17761 tggataacca gattcgcaag aatcgcgtcc gctggctccg tcaggcgctc gtcgacgcgg
    17821 gcatgaaacg cgccaatgag ctgggctggc ccaacacata cacgttcaca aagagcttgt
    17881 ccgaatcact gatacggaat ttcttggacg cttcaccgca cgccgccatc gccgtggtgc
    17941 gtccgtccat tgttgaatcc tcggttgaaa aacccttctt gggatggaac gaaggggtca
    18001 acacctcggc ttccctgtcc tatctgctgg gaacattttt tcgtcagctg ccgaccaatg
    18061 cgagcaaatg cctcgatctc attccggtcg atctcgtgtg ccgcggaatg atactgattt
    18121 cggcggcgct cgttgaacgg cggcacgagc gcatgtacca actggccact tccgttgcga
    18181 atccttgcga catgcggcga tcaattgagc tgaccggact cggccaccgc aagtattacc
    18241 gttcgcagaa cggcttccag catcggctgc ggtcgaaatt cgatgcgatt ccggtgtcca
    18301 aagctcgcta tgaaaagctg tccgcgccgg cgcaaaaaat tatcgtgcaa gccatcaacc
    18361 gcactgtcga gccgataccg ttcgttcgtc cgcctctggc gaggcaggag cgcgaattgg
    18421 agaaagtgat caagctgatt gcgctcttcg agccgttcat tctgcacaac gatcacgtat
    18481 tcgaggccat caacgtcgag agcttgagcg cagctctgcc tggggatgaa cgcgccgcct
    18541 tcggctacga tgcgcgatcc atcgactggt gggactactg gatcaatgtc cacattccgg
    18601 cgctgcgcaa gtggtgctat cctctgatcg aagggcgtca gccggaaaac ggcccgcggc
    18661 gcaatgtcgg cttcatcagc cgcactgcca cagagatcgc ggactaaccg cgcgattccc
    18721 ggcaacgatt ccctcacaac cgaaaacgtg cctattttcc tcactggctc gaccggctat
    18781 atcggcgccc atgttgcgac aaatctcctt gtcgcacagg gacagactct caacctgttg
    18841 gttcgcgccc gcgattcgca ggaagtgcgc ggccgcttgt ggcatgccct ccagcttcat
    18901 atggacttgc cgcagttcga agagctagtt gactcgcggg tgcatatttt cttgggcgac
    18961 ctcaccgccg agcattttgg actcgccggc gacgactatc acaaacttgt ccgctccacg
    19021 gattcggtga ttcactgcgc cgcatcactg aatcgcaagt ccgaaaagag ctgcctgaac
    19081 gtcaaccttc gcggcaccct cgaagtgatc caattggccg cgcgctctcg cgatgatcac
    19141 ggactgcgcc gcttcagcca cgtcagcacg gttgcggtcg ctggtcaccg tgctaacgag
    19201 gtcgttcaag aggacgaggc catcgattgg aaccgctcgg attacgatcc ctacgcgcga
    19261 accaaaaagt tctgcgagca catgctgcgc caattattgg cggacgtgcc gcgcaccatt
    19321 tttcgcccca gcatcgttct cggcgatagc cgtcgcccgg agacaaacca gttcgatatg
    19381 gttcgcgcat tcgtatttct ggccggcctg cccgcgcttc ccttccgcgc cacggacaag
    19441 gtagatattg ttcctgtcga ctacgtcgc
//
LOCUS       DATNDT010000427        11680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2695462, whole genome shotgun sequence.
ACCESSION   DATNDT010000427 DATNDT010000000
VERSION     DATNDT010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11680
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2695462"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="VN788_13990"
     CDS             complement(<1..736)
                     /locus_tag="VN788_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21691.1"
                     /translation="MSRERVALAGALAGVILLFAGGARGGTLTAATIPASPASPQPGA
                     AFLQDKGTFRILVNGQQVGKEEFEIASRGGDWVAHDTSEIQGAGGTSRVTGTLELHAD
                     GTPAHYEWSTQGEKKATATIGFEGSTATIDLHIEGVKPYTQQFTFNSPQVVILDNNLY
                     YQYAVLASLYDWNRKGTQTFAVLVPQELTPGTVTVESAGTHDAGGKKLEELAVKSPDL
                     EVDLFLDGQRLVRIVSPSANAEILRDK"
     gene            complement(733..1278)
                     /locus_tag="VN788_13995"
     CDS             complement(733..1278)
                     /locus_tag="VN788_13995"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HXU21692.1"
                     /translation="MSNGIPPRRLVCLTGFMGSGKSTAARLLASQLGWMHTDLDRCIS
                     DAAGLTISEIFARAGEAEFRALESDQLARIVAEYSQTRKPRILSLGGGTITQPKNLTL
                     LREHGAALIWLRCPIEELLRRCAQITDRPLFRDEASFRRLYTERLPTYELADYRVHSD
                     AEPPRVVEQILALGIFPEVTV"
     gene            complement(1314..2189)
                     /locus_tag="VN788_14000"
     CDS             complement(1314..2189)
                     /locus_tag="VN788_14000"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HXU21693.1"
                     /translation="MSTPSSLSGSLNWKVAGVLVPGLLLMSALPASADKIQRHFKVDA
                     RPVVTIHNPNGAITVRAWTKSEVMVVATRATDQVVVDAEQTGNRVDILTRQVSESASP
                     ESTRADYEISVPEDAELQIHDDSGGVNVSNVLGDMNVETIAADVDMADVAGYLTVKTV
                     GGSFQCARCAGRLEVSSITGNFRLIDLRSTYVRAQTSEGNILFTGQFLPNSTYRLKNY
                     NGVIEVRFSPEDSFDLSATSLKGKVHNEAKLVPPSHHHLIPHFGNGLFGSYNSGRAMV
                     ELSSFDGTINILKRN"
     gene            complement(2186..2848)
                     /locus_tag="VN788_14005"
     CDS             complement(2186..2848)
                     /locus_tag="VN788_14005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21694.1"
                     /translation="MSGANCSAFLEQLDQWMEGERSAAAEAHIRSCAECSDLIADLDS
                     IRDTASSLTATDPEPSERLWSALRAQLEDEGLIHAANPGIADSLERWLRGTFWVQPRP
                     VLAGAYLVALIAVGFALSGSSSLRNDSDLWMSRTQHSTQPLSASLETEEDAISSIADT
                     NPVVTASLRRSLAVIDNNIALCEKSVREEPQNEFARDYLYEAYQQKADLIAQVTEGDE
                     GQ"
     gene            complement(2852..3487)
                     /locus_tag="VN788_14010"
     CDS             complement(2852..3487)
                     /locus_tag="VN788_14010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU21695.1"
                     /translation="MARTRRTETGEVPEAVSIRLAQQGDAAAFERLYRLHSRRVYSLC
                     LRMVGNTAEAEDLTQEAFLQLFRKIATFRGESAFSTWLHRLAVNVVLMKLRRKSGKET
                     SLEQVTEPDEESGTPRRDFGTIDLRLSGSLDRVNLQRAVDQLPPGYKAAFILHDVQGY
                     EHNEIAEMLGCSIGNSKSQLHKARMRLRDLLQEAVREKARQERRAAGASED"
     gene            3903..5255
                     /locus_tag="VN788_14015"
     CDS             3903..5255
                     /locus_tag="VN788_14015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HXU21696.1"
                     /translation="MRISVIGTGYVGLVTGACLAEIGHDVVCTDSDASKIQILESGGI
                     PIYEPGLEKIIPKARKDGRLNFRANPADAVSAGDAIFICVGTPPLPNGDADLSAIDLV
                     ARMVATEARSPKLVVEKSTVPARTGQELKRALSVYGRKTNVKFSVASNPEFLREGTAV
                     GDFLHPDRIVVGVDDEMTERQLRDIYRPVLERKFTCPVHDGNCPGGPVPGWLVTTINS
                     AELIKHASNSFLALKISYANMVADLAERLGADIGEVVRAMGLDPRIGPSFLSAGLGFG
                     GFCLPKDLQAFVHLAERSGVDFTMLREAERINKGRIDRFVEKLRSALWVVRGKQMGVL
                     GLAFKPNTDDIRFAPAIDLVQRLLSEGAKVRAYDPEAMDRARAVLPQIDYAKSPYEAA
                     EDAEALLIATEWDEFRKLDWDRIREAMARPLIVDGRNLLSPREMKSHGFEYRSFGRPD
                     "
     gene            complement(5283..6233)
                     /locus_tag="VN788_14020"
     CDS             complement(5283..6233)
                     /locus_tag="VN788_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HXU21697.1"
                     /translation="MRVVITGGAGFLGSHLCDLFIRRGWDVLCIDNLITGARSNISHL
                     LSNPRFVFTEHDVTRYIEVPGTVDAVLHFASPASPPDYLKAPIQTMKVGSLGTHNALG
                     LALHKRAKFLQASTSECYGDPTVSPQPETYWGNVNSVGPRSVYDEAKRFSEAITMAYH
                     RHHGVDTRIVRIFNTYGPRMRLNDGRALPNFLYQALVGEPITVYGDGKQTRSFCYVSD
                     LIDGIYRLLESEEHEPVNIGNPNEITILEFAERVRALVGATAPIVFRPLPQDDPKQRC
                     PDIGKARRLLNWEPKVVLGEGLKITYDFFRQQLAAPKAGS"
     gene            6405..7511
                     /locus_tag="VN788_14025"
     CDS             6405..7511
                     /locus_tag="VN788_14025"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009458687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HXU21698.1"
                     /translation="MKVAVLDLKAEYAQLRDEILPALDRVCQNSSFVIGPEVESFERE
                     FADFCGTRHCVALSSGTAALHLGLLALGVQADHEVITSPNSFLATAEAISYCGAVPVF
                     VDIDPTNGNLDPRLIERAITPRTRVILPVHVYGRPADMDPIREIAARHNLRVLEDAAQ
                     AHGARYRGKRAGGLAHAAAFSFYPTKNIGAYGEGGALTTDDDQIAKFARAARSHGQTA
                     RYEHEFVGYNYRMQGFQGAVLRIKLRHLYAWTSRRQEIAREYRRMLANARLEIPADDP
                     RDECVYHQFVIYTDNRNATVSQLAELDIETVVHYPRPIHLQPAYSSLGLPPGTFPCAE
                     RACERVLSLPLHPGLTPEQIAYVANAVREIVGEK"
     gene            complement(7540..10071)
                     /locus_tag="VN788_14030"
     CDS             complement(7540..10071)
                     /locus_tag="VN788_14030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21699.1"
                     /translation="MLKSVAMVVHGFDLLGQAFEEHTSTIALNLQGCRYASKHHLPKN
                     TWVTLEVPEDPGHRSVRARVAWIQRPHSVREFFQVAVELESPANIWNIDSPPADWKLE
                     ETSGAAEESVISGKQDADKNSERGGESPITADANAPVEERAPSDMKILSWNSGSDKSS
                     EDAERAAGTNPLLKSWQSEMDQQVIRATETAVTRATEKIREAAEQFQREQHDAQRTFS
                     EELASKERLLTELRSEFERSARQSRELSEDLERTAAALRAEKEAAIESAARMSQARLG
                     MEAAEASLDKRVPEPAKDGAALAAGESADLRGHLDSEIARAKTQWDELLQSSLDSSIE
                     RLVGEISRHSQDVLRATEQRMSERVAELRQPFGTMQSEARDALAELKPALDQELGRVR
                     SALAEMEQAASRVKEYSAELDATTHNALDELHRRLENILQSQTEELNRRGESLAAGVP
                     QRLGSTLDALGNHLVERVTQEVETKVAPRLEHASELVRHLTARELEGEESLRLHRERM
                     RQVSESSQREAAGQAAATLASLRNDFEEARKEALIRWSEELEAAGVRASHTAAESIGR
                     SSEWFQQEARARLQVLTEQALAEAGASLEEKTATTGRHFDAALEIQSAGQLAKVYERI
                     ESVAAEIAGRTRTEIERASEAAASSFGQVLRSISEQQGQQFLSENRSAIEQRQQDLKQ
                     FASELLLNLETGAGTSIDRFHAQMASELEASVSEGRRFLSAEFASALDGYRTEREAYH
                     ADWLRNLERASDEATQKYQERLQRASDSWVDSSVRELSEHGQGAIETLVRSADQAVRD
                     SCSKLFEGFSELLRERVGNPPGRGGLGDEPAETPPQNKSSLGASA"
     gene            10311..>11680
                     /locus_tag="VN788_14035"
     CDS             10311..>11680
                     /locus_tag="VN788_14035"
                     /inference="COORDINATES: protein motif:HMM:NF015043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HXU21700.1"
                     /translation="MPSVYLEELSAVGAVRFPDIPIYQGPGAPMRLEADLRDCEVTFG
                     EVPKDFNGILYRCGPDRQYPPMGGDDIFIDGEGMVVMFRFENGHVDFKSRYVRTERFR
                     IQEKHRRALFGRYRNRYTRDPIAQNCNPGTANTNVVWHAGRLLALKEDDMPYELNPYT
                     LETVGRYDYNGAIKSVWMSAHPHLDQTTNELLTFAYQAKGDGTTDVAYFVIGPDGKLK
                     HEVWFNAPWAGMVHDFAISDKYVIFPFFPLLTDVDVLKKGGPFYTWHRDRPTVVAIVP
                     RYGTADQVRWFRGPTSFAGHMMNGSTDGSKVSLDVCLSQGSSFFFFPNHDGTPPDPAQ
                     GTPHLTRLTFDLAGNTEEYKTVQLYDFMCEMPRADDRYYGNGTYRKGYVICRDPSVPG
                     AGNIGFSSVGQFDHKTGNAKVWNPGENSGVQEPLFAPRKPNSPEGDGYLLALVNRMAE
                     NRTDLA"
BASE COUNT         2297 a         3554 c         3318 g         2511 t
ORIGIN      
        1 acttgtcgcg gaggatttca gcgttggcgc tgggggaaac gatgcggacg aggcgctgcc
       61 catcgagaaa aagatcgact tccaggtccg gacttttgac cgcgagttct tcgagcttct
      121 tcccgccggc atcgtgcgtc ccggccgatt caacagtaac agtgccgggc gtcaattcct
      181 gcggcacgag caccgcgaac gtctgcgtcc ccttccgatt ccaatcgtaa aggctggcga
      241 gcacggcata ctggtagtac aagttgttgt cgagaatgac cacctgcggc gaattgaatg
      301 tgaattgctg cgtataaggt ttcacgcctt cgatgtgaag atcgattgtc gcggtggaac
      361 cttcgaagcc gatcgtcgct gtagctttct tttcaccttg agtggaccac tcgtagtgcg
      421 cgggcgtgcc gtcggcgtgg agctcgagag tgccggtaac acgcgaggtt ccgccggcgc
      481 cctgtatttc cgatgtgtcg tgagctaccc aatcgccacc gcgcgaagcg atttcgaatt
      541 cttccttgcc tacctgctgc ccattcacaa gaatccgaaa cgttccctta tcttgtaaga
      601 aagccgcgcc cggctgtgga ctcgccggcg aagcgggaat ggttgcggcc gtcagcgtcc
      661 ctccgcgcgc gccaccagca aaaagcagaa tcacgccggc aagggcgcca gcaagtgcca
      721 cgcgctctcg gctcatacgg tcacctcggg aaaaattccg agcgccagta tttgctccac
      781 aacgcgcggc ggctccgcgt cgctatgcac cctgtagtcc gctagttcgt aagtcggcag
      841 acgctcggtg tagagccggc ggaaactcgc ttcgtcgcga aataatggac ggtccgtgat
      901 ctgggcgcag cgtctcagaa gttcctcaat tgggcagcga agccagatta gcgcggcacc
      961 gtgctctcgc agcaatgtca ggttcttcgg ctgagtaatc gtgccgccgc cgagggaaag
     1021 gatgcgcggt tttcgcgtct gcgagtattc tgccacgatg cgcgcgagtt ggtcgctttc
     1081 gagtgcgcgg aattcggctt cgcccgcgcg ggcgaaaatc tcggaaatcg taaggccagc
     1141 agcgtcgctg atgcaccgat cgaggtccgt atgcatccat ccaagttggc tggccagcag
     1201 acgcgcggcg gtcgatttac cactacccat gaatccagtc aggcagacca gccttcgagg
     1261 gggaattccg ttgctcatcg tttcggataa ctctttgtta tgcgacagcg aaatcagttc
     1321 cgcttgagga tgttgattgt accatcgaag gagctgagct cgaccatggc gcgcccggaa
     1381 ttataagacc cgaagagccc attgccgaag tgggggatta aatggtgatg agaaggggga
     1441 accagtttgg cttcattgtg caccttgccc ttcaacgagg tcgcactcaa gtcgaacgag
     1501 tcctcgggtg agaagcgcac ttcgatcacg ccgttgtagt ttttcagccg gtaggtcgaa
     1561 ttgggcagga attggccagt gaaaagaata ttcccttcgg aagtttgcgc ccgaacgtag
     1621 gtgctgcgca gatcaatcag ccgaaaattc ccggtaattg aggaaacttc aaggcggccc
     1681 gcgcatcggg cgcattggaa cgagccgccc accgtcttca ccgtaagata gccggcaacg
     1741 tcggccatat ccacgtccgc ggcgattgtt tcgacgttca tgtctcccag aacattcgaa
     1801 acgttcacac cccctgaatc gtcgtgaatc tgcaactcgg cgtcttcagg aacgctgatt
     1861 tcataatcgg cgcgcgtcga ttcgggagaa gccgattccg agacttgccg ggtgaggatg
     1921 tcgacgcgat tgccggtttg ctcggcgtcc accaccacct gatccgtggc gcgtgtcgcg
     1981 acgaccatga cttccgattt tgtccacgcg cgcacggtga tcgcgccatt cggattgtga
     2041 atggtcacaa cgggccgcgc atccactttg aaatgacgct ggatcttgtc agccgaggcc
     2101 ggcagcgcgc tcatgagcaa caagccgggg acgagcactc cggccacttt ccaattcaag
     2161 gatcccgaca gactggatgg agtgctcatt ggccctcgtc gccttccgta acctgtgcaa
     2221 tcaagtctgc cttctgctga tacgcttcat acagatagtc gcgggcgaac tcgttttggg
     2281 gttcctctcg cacgcttttt tcacacaaag cgatgttgtt gtcaatgacg gcgaggctcc
     2341 tgcgcaggga agcagtcacc accgggttgg tgtcggcaat ggatgaaatc gcgtcctcct
     2401 cggtctcaag gcttgcgctc agcggctgcg tcgagtgctg cgttcggctc atccagagat
     2461 cgctgtcgtt ccgcagactg cttgagcccg agagcgcaaa tcccactgca atcagcgcca
     2521 caagataagc cccggccaac accggacgcg gctgtaccca aaatgttccg cggagccagc
     2581 gttccaagct atccgcgatc cccggattcg ccgcatgaat cagcccctca tcctccagtt
     2641 gggcgcgaag agcgctccat agccgctcgg aaggttcagg atcggtcgcg gttagggatg
     2701 aagcggtatc gcggatcgag tccaggtcgg cgatgagatc gctacactcg gcgcaactgc
     2761 gaatatgggc ttcagcggcg gcagagcgct ccccctccat ccactggtcg agttgttcca
     2821 agaatgcgct acagttcgca ccactcatta atcaatcctc gcttgcgccg gcggctcgcc
     2881 gctcttggcg agccttctcg cgcacggcct cctggagcaa atcgcggaga cgcatgcgcg
     2941 ccttgtgcaa ttgcgacttc gaattcccga tcgaacaacc cagcatttcc gcaatctcat
     3001 tgtgctcata tccttgcaca tcgtgcagga tgaacgctgc tttatagccc ggcggcagct
     3061 ggtccaccgc gcgttgaaga ttcacgcggt cgagcgagcc gctcagcctc aaatcgatcg
     3121 tcccgaaatc ccggcgcggc gtaccggatt cttcatcagg ctcggtgacc tgctccaggg
     3181 aagtctcctt gccggacttc ctccgaagct tcatcagcac gacgttcact gccagcctgt
     3241 gcagccatgt cgaaaaggcg gactcacccc tgaatgtggc gatcttacgg aagagttgga
     3301 ggaacgcctc ctgcgttaga tcctctgctt ccgccgtatt cccaaccatt cgaaggcaca
     3361 aggagtaaac tcggcggctg tgcaagcggt ataagcgctc gaaagccgcc gcatcgcctt
     3421 gctgggcgag ccggatggac accgcttccg gtacttcccc agtttcggtt cgtctcgttc
     3481 gggccacgct acccgtcggg acgcatcctt cactgcgatg cgtcggccgc tccagaagtt
     3541 gtcctcgcgt ttcctgctgc gcaacttatc acccggtggg acggacgatg tcgcctttgc
     3601 aagcagtccc tttgagggcg ctcggaatcc ccggcaagtc tcgcctattt tcgatggaat
     3661 tgcatctgga gtccagccat gcgccgcgat tcagcgttgg ttcgggcgac tctttgtacg
     3721 acgcattggc gacgaggctt tgcgctcgac tagtgcgagc gcgacggaaa agactatacc
     3781 tggacctggc ttgcaacccg tgtcagcgaa aaactcgaaa gccaaaattg ttttggccgt
     3841 ttgtgtcagc tatagcaaaa tgaaatagca atgaacgcga gtcaagtcag tgggaggtcc
     3901 ttttgaggat ttcggtgatc ggaactgggt acgtcgggtt ggttaccggc gcatgcttgg
     3961 cggaaatagg gcacgacgtt gtgtgtaccg atagcgatgc gagcaagatt cagatcctcg
     4021 agtccggtgg aattccaatc tatgaaccgg ggctcgagaa aataattccc aaggctcgca
     4081 aagacggccg gcttaacttt cgtgcgaatc ctgctgacgc cgtgtccgcg ggcgatgcga
     4141 ttttcatctg cgtaggaact ccgcccctcc cgaatggcga tgcggacctg agcgcaatcg
     4201 atttggtggc gcggatggtg gcaacggaag cccggtcacc aaagctcgtc gtggagaaga
     4261 gcactgtccc cgcgaggacc gggcaggagc ttaaacgcgc gttgtcggtt tatggccgta
     4321 agaccaatgt gaaatttagt gtcgcttcaa atcctgaatt ccttcgcgag ggtacggctg
     4381 tcggggattt cctgcatccg gaccgcatcg tggtgggcgt ggacgacgaa atgacggagc
     4441 ggcagctccg ggacatttac cgtcccgtgc tggagcggaa attcacatgt cccgtgcacg
     4501 acggcaactg ccccggcgga cctgtgcctg gatggctggt gacgacgatc aatagcgccg
     4561 agttgatcaa gcacgcctcg aattcgttcc tcgccttgaa aatttcctac gccaatatgg
     4621 tggccgatct tgccgagcgg ctgggcgcgg atatcggcga ggtggtgcgg gcgatgggcc
     4681 tcgatcctcg gatcggtccc agctttcttt cggccggctt gggttttggc ggcttctgcc
     4741 tgccgaagga cttacaggca ttcgtgcacc ttgccgagcg ttccggcgtg gacttcacca
     4801 tgttgcgtga ggcggaacgc atcaataaag ggcgcatcga tcgctttgtt gaaaagttgc
     4861 gcagcgcgct ttgggtcgta cggggtaagc agatgggcgt tctagggctg gcctttaaac
     4921 ccaataccga cgatatccgc tttgcaccgg ccatcgattt ggttcagcga ctcctttccg
     4981 agggcgcgaa ggttcgcgca tacgatcctg aggctatgga ccgggcgcgc gctgtcttac
     5041 cgcagatcga ttatgcgaag tcgccctacg aagcagcgga agatgccgag gcacttctga
     5101 tcgccacgga gtgggatgag tttcgaaaac tggattggga caggattcgc gaggcgatgg
     5161 cgcgaccgtt gattgtggac ggacgcaact tgctttcacc gcgggaaatg aaaagccacg
     5221 ggttcgagta ccgctcgttc ggccggccgg attgatggtt cggcccgcga agccgggaaa
     5281 aatcagctcc cggccttcgg cgccgcgagt tgctggcgga aaaaatcgta tgtgatcttc
     5341 aggccttcac ccagcaccac cttcggctcc cagttcagaa ggcggcgcgc tttgccaatg
     5401 tcaggacaac gctgcttggg atcgtcctga ggcagcggac gaaacacgat gggagccgtg
     5461 gcgcccacca acgcgcggac gcgttcggcg aactccagaa ttgtaatttc attcggattg
     5521 ccgatattga ccggttcgtg ctcttcggac tcgagcaacc gatagatgcc gtcgatcaaa
     5581 tcggaaacgt agcaaaaact gcgcgtctgt tttccatcgc cgtagaccgt gattggctcg
     5641 ccgaccagcg cctgataaag aaagtttggc agcgcgcggc cgtcgttgag gcgcattcgt
     5701 ggcccatacg tgttgaatat gcgcacgatg cgcgtatcca cgccgtgatg gcggtgatag
     5761 gccattgtga tagcctcgga gaatcgtttc gcttcgtcgt acacgctgcg cggtcccacg
     5821 ctgttgacgt ttccccaata tgtttccggc tgcggactga cggtcgggtc gccgtaacac
     5881 tccgatgtgg aggcctgaag aaatttcgcc cttttgtgca gtgcgagacc cagggcgttg
     5941 tgcgtcccta gcgagccaac cttcattgtc tggataggag ctttcaggta atccggcggg
     6001 ctcgcaggag acgcgaaatg gagcactgca tccaccgtgc ccggaacctc aatgtatcga
     6061 gttacgtcgt gctcggtaaa gacgaaccga ggattcgaaa gcagatggga gatattggaa
     6121 cgggcccccg ttatgaggtt atcgatgcat agcacgtccc agccccttcg gatgaagagg
     6181 tcgcagagat gggaccctag aaatcctgcc ccgccagtga tcacgactcg caatgtgcgt
     6241 cctttccgtg cctctcgtat acgaattgtc attatccacg gaaaccactg atcgacgaag
     6301 ggtttgaatt catattgagg gccagttgca catgccctgg agcacacctt gcggacgcgg
     6361 ctcctgtaca gtacagtttt cgtccggcgc aagggcgccg cttcatgaag gttgctgttc
     6421 tagatctcaa agccgaatac gcgcagttga gggacgaaat cctccctgcg cttgaccgcg
     6481 tctgccaaaa ctcctccttt gtgatcggcc cggaagtgga atccttcgaa cgtgaattcg
     6541 ccgacttctg cggcaccaga cactgtgtcg cacttagctc gggaaccgcc gcgctgcatt
     6601 tgggattgct ggcgcttgga gtgcaagccg accacgaagt aatcaccagc ccaaatagct
     6661 ttctcgccac ggcggaggcc atcagttatt gcggagcggt gccggtcttc gtcgatatcg
     6721 accctaccaa cggaaacctg gacccgcggc tgatcgagcg agccattacg ccgcgtacgc
     6781 gcgtgatcct tccagtccac gtttacggac gtcccgctga tatggatccc atccgcgaaa
     6841 ttgccgcgcg tcacaatctt cgtgtgctcg aagacgccgc gcaagcccac ggcgcccgct
     6901 atcgcggaaa acgtgccgga ggacttgcac acgccgcggc tttcagtttc tacccgacca
     6961 aaaacatcgg cgcatatggc gaaggcggcg cgcttaccac ggatgacgat cagatcgcga
     7021 agtttgcgcg cgccgcgcgc tcccacggcc agacggcacg ctacgaacac gaatttgtcg
     7081 gttataacta tcgcatgcaa ggatttcaag gcgcagtgtt gcgaatcaaa ctccgtcacc
     7141 tttacgcatg gacctcacgg cgccaggaaa tcgcccgcga ataccgccgt atgctcgcca
     7201 atgcgcggct ggaaataccg gcggacgatc cccgtgatga atgcgtttac caccagttcg
     7261 tgatttacac ggacaaccgc aatgccactg tttcacagct tgcagagctc gacatcgaaa
     7321 ccgtggtgca ttatcccaga ccgatacacc tgcaacctgc ctattcctcc ctcgggttgc
     7381 cgccgggaac gttcccctgc gccgagcggg cgtgcgagcg cgtgctcagt ctcccgcttc
     7441 atccaggatt gacgcccgag cagattgcgt atgtggcgaa tgccgtgcgc gaaatcgtcg
     7501 gagagaagtg atccttagca gcccaagcct ttgcgaagtt taagcgctag cgccgagcga
     7561 tgatttattc tggggcggcg tttcggctgg ttcgtcgccg agacccccgc gccccggggg
     7621 attccctacc ctctcccgca gcaactccga aaatccctcg aagagtttcg agcatgaatc
     7681 gcgaactgct tgatccgccg aacgcaccag cgtctcgatc gcgccctggc catgctcgct
     7741 aagctcgcgc accgatgaat caacccagga atcgctcgct ctctgtagcc gctcctggta
     7801 tttttgggtc gcttcgtcgc tcgcgcgttc caggttccga agccagtctg cgtggtaggc
     7861 ctcgcgttcg gtgcggtaac catccagcgc ggaagcgaac tcggcgctaa gaaaccgtcg
     7921 gccctcgctg acgctggctt ccagttccga agccatttgt gcatgaaagc gatcgatgga
     7981 agtacctgcg cccgtctcca ggttgagcaa aagctcggaa gcaaactgct ttaaatcctg
     8041 ctggcgttgc tcaatggcgc tgcggttttc gctcaaaaat tgctgtcctt gctgttcgga
     8101 aatgctccga agcacttgcc caaacgacga ggcggcggct tccgaagccc gctcaatttc
     8161 cgtacgtgtg cgccccgcaa tttccgcggc gacgctctcg atgcgctcgt atactttcgc
     8221 caattggccg gccgattgta tttctagcgc cgcgtcgaag tgtcgcccgg tggtcgcagt
     8281 tttttcttcc aagctcgcgc cggcttcagc gagcgcctgc tccgtcaaca cctgcaagcg
     8341 tgctcgcgcc tcctgctgaa accattctga cgatcggccg atggactccg ccgcagtgtg
     8401 cgaggcgcga actccagccg cctctaattc ctcgctccac ctgataagcg cttctttccg
     8461 cgcctcctcg aaatcgttcc gaaggctcgc gagcgtggcc gccgcctgtc ccgcggcctc
     8521 ccgttggcta ctttccgaga cttgacgcat ccgctcgcga tgaagccgga ggctttcctc
     8581 gccctcgagc tcgcgtgccg tcaggtgccg tactaattcc gaagcgtgct ccagccgggg
     8641 cgcaaccttg gtctccacct cttgtgtcac gcgctcgacc aaatgattgc ccagggcatc
     8701 gagcgttgat cccaggcgct gcgggacgcc cgccgccagg ctttcccccc gccgattcaa
     8761 ctcttccgtc tgtgactgaa gaatgttttc cagccgccgg tggagttcgt cgagcgcatt
     8821 gtgagtcgtg gcgtccagct cggccgaata ttccttcacg cggcttgcgg cctgttccat
     8881 ctctgccagg gcggagcgca cccgccccaa ctcctggtcg agtgccggct tcaactcagc
     8941 aagcgcatca cgagcctccg attgcatggt tccaaagggc tggcgcaatt ctgcaacgcg
     9001 ttcggacatt cgctgctcag tagcgcgcaa tacgtcttga gaatgccgcg atatttcgcc
     9061 gactaggcgc tcgatgctgc tgtcgagcga cgactgcaga agttcgtccc actgtgtctt
     9121 ggcgcgcgcg atctcggaat ccagatgtcc ccgtagatct gcactttccc cggccgccaa
     9181 agcggcaccg tccttcgcgg gttccggaac gcgcttatca agcgacgcct cggctgcttc
     9241 cattcccaag cgcgcctgcg acattctcgc ggctgactca atggccgctt ctttttcggc
     9301 gcgcagcgct gctgcggtcc gctccagatc ctcactcagc tcgcgtgact ggcgagcgct
     9361 ccgctcgaat tccgaacgca gctccgtcag caatcgctcc ttggaagcaa gctcctccga
     9421 aaacgtgcgt tgcgcgtcat gctgttcgcg ctggaattgt tctgccgcct ctcggatttt
     9481 ttcggtggct cgtgtaacgg ctgtctccgt cgcgcggatc acctgctggt ccatctcgga
     9541 ttgccagctc ttcaggagcg ggttcgttcc cgccgcacgc tctgcatctt cggatgattt
     9601 gtctgaacct gaattccacg atagtatttt catgtcagat ggggcccgtt cctctactgg
     9661 cgcgttggcg tcagctgtaa tgggagattc cccgcctctc tcagaatttt tgtccgcgtc
     9721 ttgcttccct gatatgacag attcttccgc cgccccggaa gtttcctcca acttccagtc
     9781 tgcggggggt gaatcgatat tccaaatgtt cgccgggctc tccagctcta ctgcaacctg
     9841 aaaaaactcc cgtaccgagt gaggccgctg aatccacgcc acacgcgcgc gtacgctccg
     9901 gtgcccggga tcctcgggaa cctccagggt cacccaggta ttttttggca ggtgatgctt
     9961 cgacgcgtag cggcagcctt gaagattcag tgcaatggtc gaggtatgtt cctcgaatgc
    10021 ctggccaagc aagtcgaatc cgtgaaccac cattgccacg gactttagca ctcgctcact
    10081 gcgccgccgc tgtgtgctcg tgtgactcgc gtgagattct gacacggcgg tcctcgcaaa
    10141 ctacctgcga gagaataaca ccgtcgcagc cttcggtcga gagcagcccc gagcgtctaa
    10201 aatcattgcg cagactacgc gcgttgacaa atcagtttgg ttgcgactag cattcttcgc
    10261 agcactcaaa aagcgtaggc ccaccacatc ggtgcaagca ggagttcacc atgccgagcg
    10321 tatacctcga agaattatcc gccgtaggag cagttcgctt cccggatatt cccatttatc
    10381 agggtcccgg cgcgccgatg cgcctcgaag cggacttgcg cgattgcgaa gtcaccttcg
    10441 gcgaagtgcc aaaagatttc aatggcattc tttatcgctg cggccccgat cgccagtatc
    10501 cgcccatggg aggcgacgac attttcatcg acggtgaggg catggtggtc atgttccgct
    10561 tcgagaatgg ccacgtggac ttcaaaagcc gctacgttcg caccgagcgc ttccgcatcc
    10621 aggaaaaaca tcgtcgcgca ctcttcggcc gctatcgcaa tcgctacacg cgcgatccca
    10681 tcgcccagaa ctgcaatccc ggcacggcca acaccaacgt cgtctggcac gccggcaggc
    10741 tgctcgccct caaagaggac gacatgccct acgagctgaa tccctacacg ctcgaaaccg
    10801 tcggccgcta cgattacaat ggcgccataa aatccgtatg gatgtccgct catccgcatc
    10861 tcgaccaaac cacgaacgag ctgctcactt tcgcctatca ggccaaaggc gatggcacca
    10921 ctgacgtcgc gtacttcgtc attggccccg acggcaaatt gaagcacgaa gtttggttca
    10981 atgcgccgtg ggccggcatg gtccacgact tcgccatctc ggataagtac gtcatttttc
    11041 cattctttcc gctgctcacc gatgtggacg tgctgaaaaa gggcggcccc ttttacacgt
    11101 ggcatcgcga ccggcccacc gtcgtcgcga ttgtcccgcg ctacggcact gcggaccaag
    11161 tccgctggtt ccgcggcccc accagtttcg ccggccacat gatgaacggg tcaaccgacg
    11221 gctcaaaggt gagtctcgat gtgtgtctat cgcagggaag ttcgtttttc ttcttcccca
    11281 atcacgatgg cactccaccc gaccctgcgc agggtacgcc tcatctcacg cgtctgacct
    11341 tcgatctcgc cggcaacacc gaggaataca aaaccgtgca gctttacgat ttcatgtgcg
    11401 agatgccccg cgccgatgac cgctactacg gcaacggaac ataccgaaag ggctacgtca
    11461 tctgccgaga tcccagtgtc cccggagcgg ggaacatcgg attcagctcc gtgggccaat
    11521 tcgatcacaa gacgggcaac gcgaaggttt ggaacccagg agaaaattcg ggggtgcagg
    11581 aacctttatt tgctccgcgc aagccgaatt cgccggaagg cgatggctac ctgctcgcgc
    11641 tcgtcaatcg catggcagaa aatcgcaccg acctcgcgat
//
LOCUS       DATNDT010000428        11367 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2387369, whole genome shotgun sequence.
ACCESSION   DATNDT010000428 DATNDT010000000
VERSION     DATNDT010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2387369"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..369)
                     /locus_tag="VN788_14040"
     CDS             complement(<1..369)
                     /locus_tag="VN788_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXU21701.1"
                     /translation="MEFRQLRYFIAVAERLSYSKAAEELHISVSPLSRQIRQLEDEFS
                     IQLFERDRRRVSLTDAGKLFLQEAKALISHMARVTDHMQQAKKGEAGIVRAGIALHLA
                     DKVGNVVVEHSKRYPAVDIQC"
     gene            703..4791
                     /locus_tag="VN788_14045"
     CDS             703..4791
                     /locus_tag="VN788_14045"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU21702.1"
                     /translation="MKSIRVFLFSVLAAAFLIAGAQFAMAQTAETGALAGTVTDPSGG
                     VVAGASITITNTGTGQARTATTDSNGSYKFSLLPPGNYSVKFSSPGFQTAEVPSVTVN
                     VTETAVLNHALQVGGQSIQVTVEATTQAIQTENVTNGGVVTGDEITSLPLVSRNYTQI
                     INLSPGVVANAATASSIGNGTQDVSANGSTANENNYSMNGASLVNYVSGMAAQEGSFP
                     GIAIPNPDAIEEFKVQTSQYDASSGRNPGANVEVVTKTGSNNFHGRAWEFNRNNFFNG
                     NDFFFKRSEAALGEPNKPQTLKQNTFGFTLGGPIKKNKAHFFGSYQGFRQLNGIGTSG
                     FASGYAPNVQLLPWNDYADFASGVCSDLRCTNNVAAYKTYLGSVFGTQTGFLTFLGGT
                     GLPATSANITNTAVALLQAKGTVKGGFNQGFYFPAAPQSCALNPITGACGPVAISDPT
                     RANEDQYIINGDYSISQKNTLYARYLYQRDPQLQPFNCFILAGNCNPGAPINAYYGNH
                     IAQLQLTSVLTPNLVNQARADYHRDIENNTDPNLGVQSCSLPNGGTIIPLVNNGAPCG
                     SAVPALAKQFPEMLVVPQLDILGIGGAPWSQGGNFSMISSNFINTFQWSDQVSWNHGI
                     HSLRFGFEGNRVLYNNTIPSSGRGELLMYSTADFLTSSSGLAVDGTPVTPTGGIALGF
                     GLKGTLTHYNRVNQFDWYIQDDIKLTPKLTLNAGVRWEFAGYPDDKSGQFSNVWASQL
                     TKINTGSAFASLGNTGTLVGFAVPSNFAVKTFGLTAPSGANGVLVNGNKTLVPGTPWK
                     DFAPRIGVAWQPIGQKLVVRAGYGWFYDTIYGNLLIDNQLNLPPYSGAGGGPAPLNQE
                     NTLHAPWRAGVGPLVWTPRYMFTGLFDPNTGALCPAGICSSGLGYTSDSPQLADRLPL
                     IQEYNLDLQYEFAHGWVADVGYVGSHGIHLYDWSRNVNIGRLVAGAPNNPTAASGFQN
                     MEMIASSLPYNDPANPTPITTNTIGTTTSNLNERVSYLGFSPAGVAETNTLGDHLYNS
                     LQAQLRHQFSNGLLLQAAYTWSKEFTNINTSAAGSGIQPPGEVIFGAANSNDPLDLRQ
                     QYGLAAFNRSQRGVITYVYNLPYHKTEGLSGKLLGGWTFSGVTTIQNGLPFWIVDGGG
                     GSIYGAGLGGSRAALADPVDCNPKTGSCKSGIPIATSGSNTSRATSHWVNPSAFVSLS
                     PSTYAAPNPALPFGALPASSPYCIGGTPNPGLFGPPNPNEPCGQNPVPPRAPGTFGPG
                     DPGAPADPGAPFLNAGSGFGNTAIGSITGPGQFNFDMSLIKTTKLWEGGTLEFHVDAF
                     NVFNHAQFNPPGNDVNAAATFGIITSTSVTPRVMQFGLKFLF"
     gene            complement(4934..6010)
                     /gene="ychF"
                     /locus_tag="VN788_14050"
     CDS             complement(4934..6010)
                     /gene="ychF"
                     /locus_tag="VN788_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HXU21703.1"
                     /translation="MQTGIIGLPQVGKTSLFRILTKARVDARSAPNQAHVGIARVPDA
                     RVAKLAEVFKPKKVTYATIEYVDIGGLQKDREKNSASLVPLREADALAHVVRLFENPA
                     VPHEAGSLDAVRDIVNVDLELILNDLEQAAKRIERVEKDLKKKKESLLESELQLLMRC
                     RQALEAETPLRELELKPEEEKMLTGFMFLTRKPMLYVLNLGDEEATEIDRVVEKHHLE
                     KLASKRQTAVVPFCGKIEAELAELEDAESAEMMRAYGLTESGRDRLIQSSYRLLGLIS
                     FLTCGEPECRAWTIKRGMTAPEAAGAIHSDIQKNFIKADVVNWEDLLKAGTFTAAREK
                     AQVRLEGKEYVVQDGDVILFRHSG"
     gene            complement(6133..6432)
                     /gene="rpsT"
                     /locus_tag="VN788_14055"
     CDS             complement(6133..6432)
                     /gene="rpsT"
                     /locus_tag="VN788_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HXU21704.1"
                     /translation="MAQGNPVKHKKKTKSVLKNIRQTERRAAINRMNRTRVRTAMRRM
                     RAALSAADGGEAAKLASATFSELDKAIRKRTLSENTANRYKSRLTLALNTLKAKK"
     gene            complement(6533..8119)
                     /locus_tag="VN788_14060"
     CDS             complement(6533..8119)
                     /locus_tag="VN788_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXU21705.1"
                     /translation="MNSPTTAVLGEIGLPASVRELASRRWDVVVVGAGHNGLACAAYL
                     ARAGKRVLVLESRARVGGACTIEEPFPGVRMSPCAYLAGLLHPLVISELALPARGFEW
                     TPAVNGLFVPFLDGSSVQLWDDDAQCEAEIRSFAPGDVEGWRAMSDVLRRLRDALRPA
                     GDRDVWIGDAPTREQIEERLGKDDEAKRLLFEWSMAEFVEHYLRDERLQTAYLGQGVI
                     GTNASPFDAGTASIRFHHASGRLGGMPGMWGYVKGGMGMVSFYFCDAAREAGASVAAG
                     VSVAQIVPGEGVRLDGGEFIAAPVVVSNADPRQTLRLLGAAVDGAWRKRVEQIPIEGC
                     TVKLNVLLRELPSFTARPGTDQPHHYGQINIPLTKNEWKAGFTAARRGDLPKHLWCEL
                     YFQSAHDPTIASAGQHTMSVFAQYVPYKFERGTWDERREEVRRLALDSLARFCPNIES
                     AVIDAQVLGPPDIEMKVGLTGGHIFQGECLPPNMWSKRLAARTPMPGMYLCGACTHPG
                     GSVIGINGRNAAMAVLSDTA"
     gene            complement(8284..9108)
                     /locus_tag="VN788_14065"
     CDS             complement(8284..9108)
                     /locus_tag="VN788_14065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21706.1"
                     /translation="MGVRRIVALAAAVAFAIDIFCFAKSDVAQSASSTQTTPKADHPA
                     KNLQPESRLLLIRGVDGENAKLVHSLPAGKHGFMLSVGKPLDAQHLKDALRLWGTAAG
                     QGDTIQITGLEFRYKQIRVQINGGGKHHFNWRQHLQIGMGNLSTAPPPDTGPKQPLGG
                     VLILDYGRPLPDMTPEDLKHDLAVMLDFSKQQSAAVNWIDTIPPKFKQAITDHKAMVG
                     MDEDMVIAAVGRPDHKVREREPDGRETEDWIYGTPPAKTVFVTFAGDNVIRVKEFN"
     gene            complement(9252..9325)
                     /locus_tag="VN788_14070"
     tRNA            complement(9252..9325)
                     /locus_tag="VN788_14070"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9291..9293),aa:Gly,seq:gcc)
     gene            complement(9460..9684)
                     /locus_tag="VN788_14075"
     CDS             complement(9460..9684)
                     /locus_tag="VN788_14075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21707.1"
                     /translation="MAKRKKAAKSSGKRADRRRKHRHWQVTVYYSDGEKFARVYIDRD
                     RAERFADRQKKSPVVKATRILEVDRDGANS"
     gene            complement(9824..11170)
                     /locus_tag="VN788_14080"
     CDS             complement(9824..11170)
                     /locus_tag="VN788_14080"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HXU21708.1"
                     /translation="MIKKPTIIVLVCAVILGGLVYYFDWKKGNEAKPAVDASKLAFSI
                     QASDIASITLAHPAESNTPPIRLEKTGDTWKIVQPIETQADQPTVDGIADQLASARVS
                     VTEPGSADRRKVYGLDPAQVSLEFQLRNGSKHTILIGSKDFAGDSAYTVIDGAQNVSL
                     LPESLATSAGKSVEDLRDRSVLHIDAADVSSFDLTNPSGQLAASKKNDQWHFTKPAPT
                     VADPDAMDSLLQAVANAKMASIVEEKPEHLEKYGLQKPAITLTATVGKGSPAALLVGK
                     KTGSAYYARDASRPTIFTIGDDVYSKLTQKYADLRDKKVVNLAASDMQQIQLTDAAGS
                     LAVSRKKDNSEEWTFQSPEQQKGKPASGWKLVDALTGLTADQIIDHPSSADLKSLAKP
                     ETTVVVTNPKGEKMTIRFSKATGEFVLAQDSNSTSLYRLKKGAITGLALKPADLTP"
BASE COUNT         2372 a         3422 c         3011 g         2562 t
ORIGIN      
        1 gcactgtatg tctacggccg gatatcgctt cgaatgttcg acaaccacgt tgccaacttt
       61 gtccgccaaa tgcagcgcaa tgccggcgcg cactatgccg gcctctcctt ttttcgcctg
      121 ctgcatgtga tcggtcaccc gtgccatgtg gctgatcagg gctttggcct cttgtaggaa
      181 cagcttcccg gcgtcggtta acgacactct ccggcggtcg cgctcgaaaa gttggatact
      241 gaactcatct tccaactgtc gaatttgcct gctcaggggg gataccgata tgtgcaactc
      301 ctcagcggct tttgaatagc tgaggcgctc cgcgacggca ataaagtagc gcaactgacg
      361 aaattccatg gccctgaccc gatctcaccc cgattgtgta tccaaacaaa cgcattttgt
      421 atccacaaac cgaaaaccaa cctcgaaaac acagccacca ggctgctttt gcataattcg
      481 taacagaatc gctcaaaata agcaatggac ttgtaagact gaatcggttt tagttcttct
      541 cggaatgggt gggcatttgt ggtgtgggct atggcaggct tccaactttc ggagttttct
      601 cgtctatttc aggcagcctg tacgtctcgc cgtgatcgcg actcccagat actctgaatt
      661 taccctaggc ccgaccatgc gggtctgaat ccagggggga caatgaaatc gattcgagta
      721 ttcctttttt cggtgctcgc cgcggcgttc ttgatagcgg gcgcacaatt cgcaatggcg
      781 cagacggcgg agaccggcgc gctcgcaggc accgtgaccg atccgtccgg cggcgtcgtg
      841 gcgggagcca gtatcacgat cacgaatact ggaaccggtc aggcccgcac tgccaccacc
      901 gactccaatg gttcttataa attttcactt cttcctccgg gcaattattc ggtgaaattc
      961 tcgtcgccgg gttttcagac ggcagaagtt ccctcagtga ccgtcaatgt gaccgaaact
     1021 gccgtgctga accatgcgct acaggtaggc ggccagagca tacaggtcac ggtcgaagcc
     1081 accactcagg cgattcagac ggaaaacgtt acgaacggag gtgtcgttac tggagacgaa
     1141 attacttccc tgccgttagt ctcgcgtaac tacactcaga tcatcaacct ctcgccgggt
     1201 gttgttgcca acgccgccac tgcatcgtcg atcgggaatg ggactcagga cgttagcgcg
     1261 aatggctcca cggccaacga gaacaactac tccatgaacg gtgcctcact cgtgaactat
     1321 gtaagcggta tggccgctca agagggcagt ttcccgggta tcgccatccc caacccggat
     1381 gcgatcgagg agttcaaagt acagacatct caatatgatg ccagttccgg ccgcaacccg
     1441 ggcgcaaacg tagaagtcgt cacgaaaacc ggcagcaata acttccacgg aagagcttgg
     1501 gagttcaatc gcaataactt cttcaatggc aacgatttct ttttcaaacg cagcgaagcc
     1561 gcattaggcg agccgaacaa gccacagaca ctgaaacaaa acacttttgg attcactctc
     1621 ggtggtccga tcaaaaagaa taaagcccac ttctttggtt cgtatcaggg ttttcggcag
     1681 ctcaacggca tcggcaccag cggttttgcc tctggctatg cgccaaacgt tcagcttttg
     1741 ccgtggaacg attatgcgga tttcgccagc ggcgtgtgta gtgatctgcg ctgcaccaac
     1801 aatgtcgccg cctacaagac ctatcttggc agcgtattcg gaacccagac tggctttctc
     1861 actttccttg gcggcacggg cctcccagcc acaagcgcga acatcaccaa tacggcggtg
     1921 gcactgctgc aagccaaggg aacggttaag ggcggcttca accaaggctt ctattttcct
     1981 gccgctcccc agtcctgcgc tttgaaccca atcacggggg cctgcggacc cgtcgccatt
     2041 tcggatccga cgcgcgccaa tgaagatcaa tacatcatca atggcgacta ttcgatctcc
     2101 cagaagaaca cgctctacgc gcgttacctg taccagagag atccccagct tcaacctttc
     2161 aactgcttca ttctcgcagg gaactgcaat cccggcgcgc ccatcaacgc ctattacggc
     2221 aaccacatcg cgcagctcca attgacatca gtgctgacgc ctaatctcgt caaccaagcc
     2281 cgcgctgatt atcaccgtga catcgagaac aacacagacc cgaatctagg cgtccaatcc
     2341 tgctcgctcc ccaatggcgg cacgattatt ccactcgtaa acaacggcgc cccatgcggc
     2401 tccgcggtgc ctgcgctcgc caagcaattc ccggaaatgc tggttgttcc gcagctggat
     2461 attctgggaa tcggcggcgc gccttggagc cagggcggca acttctcgat gatttcgagc
     2521 aactttataa acacgttcca gtggtctgat caggtctcct ggaaccacgg aatccactcg
     2581 ctgaggttcg ggttcgaagg taaccgcgtc ctgtacaaca acaccattcc gtcttccgga
     2641 cgcggcgaac tgctcatgta ttctaccgcc gactttctga ccagcagctc cgggctggcg
     2701 gttgatggta ctcccgttac tccaaccggc ggtatcgccc ttgggttcgg cctaaaaggc
     2761 actctgacac actacaaccg cgtgaatcag ttcgattggt acatccagga cgacatcaag
     2821 ctgactccga agctcaccct gaacgccggc gtgcgctggg aatttgcggg ttacccggat
     2881 gacaagagcg gccagttctc caatgtctgg gcttcacagc taacaaaaat caataccggc
     2941 tccgcttttg catcattggg caacacaggc actctggttg gatttgccgt tccttccaac
     3001 tttgcggtga agacttttgg tctgactgcg ccttccggtg ccaatggggt acttgtaaac
     3061 ggtaataaga ccttggttcc cggcactcct tggaaggact tcgccccgcg cataggagtg
     3121 gcatggcagc cgatcggaca gaaacttgtg gtccgcgcag gttacggttg gttttacgat
     3181 acgatctacg gcaacctcct gatcgacaat caattgaacc tccctcccta ttccggagca
     3241 ggaggtggtc ctgcgcccct caaccaggaa aacactctcc acgctccgtg gcgggctggt
     3301 gtcggcccgt tggtatggac gccgcgctac atgttcacgg gcctctttga tccaaacacg
     3361 ggcgcgcttt gccccgctgg catttgctcc tcgggccttg gatacacgtc cgattcgcct
     3421 cagctcgcgg atcgtttgcc cctgattcaa gaatacaacc tcgatctgca gtacgagttt
     3481 gctcacggtt gggttgcgga cgttggttac gtgggaagcc acggtattca tctctatgac
     3541 tggtcgagga acgtcaatat cggccgtctg gtcgcgggcg ccccaaacaa tccgaccgcg
     3601 gcttccggat tccagaacat ggagatgatc gcttcttcgc tgccctacaa cgatcccgcg
     3661 aaccccactc cgatcacgac aaacacgatc ggcacaacga cctcaaacct gaacgagcgc
     3721 gtaagctacc tcggtttttc acccgctggc gtcgccgaga ccaatacgct tggcgatcac
     3781 ctgtacaaca gtttgcaggc tcagttgcgt caccagttct ccaacggcct cctgcttcag
     3841 gcggcgtaca cttggagcaa ggaatttacg aacatcaaca cttcggcggc aggaagtggc
     3901 attcaaccgc cgggagaggt gattttcgga gccgccaata gcaacgatcc tctcgacttg
     3961 cgccagcaat acggactcgc tgccttcaac cgttctcaga gaggtgtaat tacctacgtt
     4021 tacaacctcc cgtatcataa aaccgagggc ctgagcggca agcttctcgg cggatggact
     4081 ttctctgggg tgaccacgat tcagaatggt ctgccatttt ggatcgtaga tggcggaggc
     4141 ggatcgatct atggcgcagg gctaggggga tcgagagctg ccctggcaga ccccgtcgat
     4201 tgcaacccga agaccggaag ttgcaagtcg ggaattccga tcgccacttc tgggagcaac
     4261 acttctcgag ccacaagcca ttgggtcaac ccgagcgcgt tcgtctccct ctccccaagc
     4321 acctacgcag caccaaatcc agcactgccg tttggggcgc ttcctgcgtc atccccgtat
     4381 tgtattggag gtacgcccaa cccaggacta ttcggaccgc cgaacccgaa cgagccctgc
     4441 ggtcagaacc ctgtgccgcc ccgtgcccct ggcacattcg gtccagggga tcccggagcc
     4501 cctgctgacc ctggcgcacc attcctgaac gccggtagcg gcttcgggaa taccgccatc
     4561 ggttccatca cggggccagg tcaatttaac ttcgatatgt ccctgattaa gacgaccaaa
     4621 ctgtgggaag gcggcacgct tgagttccac gtggatgcgt ttaacgtctt caaccatgca
     4681 caattcaatc cgcccggaaa cgacgtgaac gcggcggcta cgttcggcat catcacctcc
     4741 acatcggtca cgccgcgcgt gatgcagttt ggcttgaagt tccttttcta ggagcgacgg
     4801 agaagttagc tcttactcgc tgttgtagct gtacccaccc cggggaccgg ttgggcccac
     4861 cccctagctg gtccctttct tttttggcgg actagccccg gaagtggttt tgcattcggt
     4921 tgattttcgg cgtttatccc gagtgtcgga atagaataac gtcgccatcc tgcaccacgt
     4981 actctttgcc ttcaaggcgc acttgggcct tctcacgcgc cgcggtgaaa gttcccgctt
     5041 tcagcaagtc ctcccaattc accacgtccg ccttgatgaa attcttctgg atgtcgctgt
     5101 ggatcgcgcc cgcggcttct ggcgcagtca tgccgcgttt gatagtccag gcgcggcatt
     5161 ctggctcgcc gcaagtgaga aacgaaatta aaccgagcag gcggtaactt gattggatca
     5221 gccggtcgcg tcccgattcc gtcaggccgt acgcgcgcat catctcggcg gactctgcat
     5281 cctccagttc cgccaactcc gcctcgatct tcccgcaaaa tgggaccacg gcggtttgcc
     5341 tcttcgacgc cagtttttct agatgatgtt tctcaactac ccgatcgatc tctgtggctt
     5401 cctcgtctcc gagattcaaa acgtatagca tcggttttcg cgtaagaaac atgaagcccg
     5461 taagcatttt ctcttcttct ggcttcagtt cgagctcgcg caaaggcgtc tcagcctcga
     5521 gcgcctgccg gcagcgcatc agcagctgca attccgattc gagcagcgat tcctttttct
     5581 ttttcagatc cttttccacg cgttcgatgc gtttcgccgc ctgttcgaga tcgtttagaa
     5641 tcaactcaag atctacgttc acaatgtcgc gaaccgcatc gagcgagccc gcttcgtgtg
     5701 gcaccgccgg attctcgaac agccgcacca cgtgcgccag agcgtccgct tcgcgcagcg
     5761 gcaccagcga agccgaattt ttctcgcggt ctttctgcaa gccgccgata tcgacatact
     5821 cgatcgtcgc gtacgtcacc ttcttcggct taaatacttc ggcgagtttc gccacacgag
     5881 cgtctgggac tcgcgcgatc cccacgtgcg cctgattcgg cgcgcttcgc gcatccacgc
     5941 gcgccttggt gagaatgcgg aacaggctcg tcttcccaac ctgcggtagc ccgataattc
     6001 cagtttgcat aagcggtcga tactagcaca gccattcacg tcggctgagt gccacgcgtc
     6061 cgtgcaggca aagaaaaagc gccccagctt gccggagcgc ttttcaaaaa cagccgaatt
     6121 aatttcggag acttatttct ttgctttaag cgtattgagc gccaacgtga gccgcgactt
     6181 atagcgattt gccgtgttct ccgatagcgt ccgcttccga atcgccttat ccaattccga
     6241 aaatgttgcc gacgcgagct tcgcagcctc gcccccatcc gccgccgaaa gggctgcgcg
     6301 catgcgccgc atcgccgtcc gcacccgggt ccggttcatg cgattaatcg ctgcccggcg
     6361 ttccgtctgc cgtatattct tcagtacgga cttggtcttt ttcttgtgtt ttacagggtt
     6421 gccctgcgcc attcattgct ccttagcttt tgatttcagc ctagcaggtt agccgacacg
     6481 cgccaccgtg tcaatttcgt cgcgcacaaa atagcaagcc gcgagtgcaa atttaggcag
     6541 tatcgctgag aacagccatt gcggcattcc gcccattgat gccaatcacg cttcctcccg
     6601 gatgcgtgca tgcgccgcac aaatacatac ccggcatggg cgtgcgcgcc gcaagccgct
     6661 tggaccacat gttaggaggc aagcactcac cctgaaaaat gtggccgccc gtcagtccga
     6721 ccttcatctc gatatcgggc ggaccaagca cctgcgcgtc gatcaccgca ctttcgatat
     6781 tcggacagaa tcgcgcaagg gaatcgagtg cgagccggcg aacttcttcc cgtcgctcat
     6841 cccacgtgcc gcgctcaaac ttgtacggaa catactgcgc gaacacactc atcgtatgct
     6901 gcccggcaga cgcaatcgtc ggatcgtggg cgctctgaaa atagagctcg caccataaat
     6961 gcttcggcaa gtccccgcgg cgcgcagcgg taaaccccgc cttccactcg tttttggtaa
     7021 gcggtatgtt gatttggccg tagtgatgcg gctgatcggt tcccggccgc gccgtgaagc
     7081 tcggcagctc tcgcagcagc acgttcagct tcaccgtgca accttcaatc ggaatctgct
     7141 cgacgcgttt ccgccaggcg ccatcgacgg cagcgcccag taatcgcagc gtttgccgcg
     7201 ggtcggcatt tgacaccacc acgggcgcgg cgatgaattc gccgccatcg agccgcacgc
     7261 cttcgccagg cacgatctgc gcgacagaaa ctcccgcggc cactgacgct cctgcctctc
     7321 gcgcggcatc gcaaaagtaa aatgaaacca tccccatgcc gcccttcacg tagccccaca
     7381 tgccgggcat tccgccaaga cgccccgaag cgtgatggaa gcggatgctc gcggttcctg
     7441 catcgaacgg gctggcatta gtcccaatca ctccttgtcc caggtacgca gtctgcagcc
     7501 gttcgtcgcg caaataatgt tcaacgaact ccgccatcga ccactcgaat agcagtcgct
     7561 tcgcttcgtc atctttcccc agccgctctt cgatctgttc gcgcgtgggg gcgtcgccga
     7621 tccacacatc gcgatcgccc gccggacgca acgcatcgcg caatctgcgc agcacatcgc
     7681 tcatcgcgcg ccagccctct acatctcccg gagcgaagct gcgaatttcc gcctcacact
     7741 gcgcgtcgtc gtcccacaat tgaacgcttg agccgtcaag gaatggaacg aatagcccgt
     7801 tgaccgcagg cgtccattcg aagccgcgcg cgggcagcgc caactcggaa atcaccaggg
     7861 gatgcagcag tcccgccaaa tacgcgcatg gcgacatgcg aacgccaggg aagggttctt
     7921 caatggtgca ggcgccgccc actcgcgcgc ggctctccag caccagcacg cgcttgcccg
     7981 cgcgcgccag gtacgcagcg caggccaacc cgttgtgccc ggccccgacc accaccacat
     8041 cccaccgccg cgaagcgagt tcgcgaacgg aagcaggcag tccaatttcg ccaagcacag
     8101 cggtcgtcgg tgaattcacg gcccgtagta tccaccgcta accatcaact tgcaaagtag
     8161 cccgaaaaaa cccgggtgcc ccatgctcgt agtgtgagcg tggggttttt gacgtgctcg
     8221 tatgtccggt agcgcagact ttggtattcc tcaccactgc aaaggcggga ctgtacgaag
     8281 gtcttaattg aactctttca cgcggatcac gttgtcgccg gcaaaagtga cgaacaccgt
     8341 ttttgcgggc ggcgtaccat aaatccaatc ctcagtttcc ctgccatccg gttcgcgctc
     8401 gcgaaccttg tggtccggac gccccaccgc cgcgatcacc atgtcctcgt ccatccccac
     8461 catcgctttg tggtccgtga ttgcctgttt aaatttcggc ggaatcgtat cgatccagtt
     8521 tacggctgcc gattgctgct tggaaaaatc gagcatcact gcgaggtcgt gcttcaaatc
     8581 ttcgggagtc atgtccggca gcgggcggcc atagtccaaa atcagaacgc ctcccagtgg
     8641 ttgcttggga cccgtgtccg gcggaggcgc agtcgaaaga tttcccatgc cgatctgcaa
     8701 atgctggcgc caattaaaat ggtgtttgcc tccgccattg atttggacac gtatttgttt
     8761 gtagcgaaat tcgagccccg taatttgaat ggtatcgcct tggccggccg ccgtacccca
     8821 cagcctcagc gcgtccttca ggtgttgcgc gtccaatggt ttgccaacag aaagcatgaa
     8881 gccatgcttt ccggccggca atgaatgcac gagttttgcg ttctcgccgt ccactccccg
     8941 aatgagcagc agccgcgatt caggctgaag atttttcgca gggtgatccg cttttggcgt
     9001 tgtctgcgtg gatgacgcgg attgcgccac gtcgcttttc gcgaagcaaa aaatatcgat
     9061 cgcgaaagca accgcggcag cgagtgcgac gatgcggcgg actcccatcc tagtcccgta
     9121 tccctattct attgcttttg gccaaattcg gggaaagttt ggcgctgcgc caagtgagat
     9181 ggcaatgcct cgccggacgt tgctcagccg ttatcaatta cttcgaagga aattcggcgt
     9241 ccggcgaaaa ttggagcggg ataagagaat cgaactctcg cctccacctt ggcaaggtgg
     9301 cgtactacca ctatacgaat cccgcacgta agaagtactt ccaataatat tctataacac
     9361 gcagcccgtc agcacgaaaa ctcaacgcaa gttcgtgcga agcgtgtgcg atgcgcccat
     9421 agcaaaaaca cgcgcgtggg aaaaaaacga atgtccccgc tacgaatttg ccccatctcg
     9481 atccacttcc aaaatccgag tcgccttgac gacgggggat tttttttggc ggtcggcgaa
     9541 acgctcggcg cggtcgcgat cgatgtaaac tcgagcaaac ttttctccat cactgtaata
     9601 aacagtgacc tgccagtggc ggtgcttgcg tcggcggtcg gcccgctttc cgcttgattt
     9661 tgcagccttt ttccttttgg ccattcttgg ttggtcctcg gtgatttgga ctattacaga
     9721 agccggacgc gcagcacaag tatctagttt gtagttgcct ttccttctaa ctcgaagcat
     9781 tttcaggtcg caaacggccc attcacgcgg cgtttgcggg cggttacggg gtcaaatccg
     9841 ccggtttcag agccaggccg gtgatggcac ccttcttaag tctgtacagg gaggtactat
     9901 tactgtcttg ggctaaaacg aactcgccgg tggctttcga aaaccggatt gtcatttttt
     9961 cacccttggg attggttacg acaaccgtcg tttcaggctt tgccagagac ttgaggtcgg
    10021 cggacgaagg gtggtcgatt atctgatcgg cggtgaggcc agtgagggcg tcaaccagtt
    10081 tccagccaga cgccggcttc cccttctgct gctcgggaga ttgaaaagtc cactcttcgg
    10141 aattgtcttt tttgcggctc acggcgagtg accctgcggc atcggtgagc tgaatctgtt
    10201 gcatgtcgga agccgcgagg ttgacgactt tcttgtcgcg cagatcggcg tatttctgag
    10261 ttagcttcga atacacatcg tcgccgatgg taaaaatcgt gggacgagaa gcatcgcgcg
    10321 cgtagtatgc gcttccggtt tttttgccga cgagcaacgc ggcgggcgag cctttgccaa
    10381 ctgttgcggt gagagtgatc gcgggttttt gcaggccgta cttttccagg tgttccggtt
    10441 tttcttcaac gatggaggcc attttcgcgt tggcaacggc ctggagcaaa ctatccatcg
    10501 catcgggatc ggcaacagtg ggcgcgggct tggtgaaatg ccactgatcg tttttcttgg
    10561 aggcggcaag ctggccggaa ggattcgtga gatcgaagga cgaaacgtcg gcggcatcga
    10621 tatggagaac gctgcggtcg cgcaggtcct cgacagattt gcccgcgctg gttgccagcg
    10681 attccggcaa cagagaaaca ttttgggcgc catcgataac ggtgtaggcg gaatcacccg
    10741 cgaaatcctt gctgccgatg agaatggtgt gtttcgagcc gttgcgaagt tgaaattcca
    10801 gagagacctg cgctggatcg agtccgtaaa ctttgcgccg atcggcactg cccggttccg
    10861 tgactgaaac tctcgcgctg gctagctggt cggctatgcc atcaacagtg ggctgatccg
    10921 cttgggtttc gattggttgg acgattttcc aagtgtcgcc ggtcttttca agacgaatcg
    10981 gcggcgtatt cgattcggca ggatgagcca atgtaatcga ggcgatgtcg gaagcctgga
    11041 ttgaaaatgc gagtttcgag gcatcgacag cgggctttgc ttcgttgccc ttcttccaat
    11101 cgaaataata aacaagcccg ccgaggataa cggcgcagac aagcacgatg attgtcggct
    11161 ttttaatcat aacgattcgc ggcttcctgt ttttaccggc gcttccacca gatcgaaatc
    11221 cccacaagga tgacgatgcc cggtaagaaa aacatctcga tccatcgcag cgaggcggcc
    11281 tgtgtttccg tgagcacgat gtgacggctg gttgccgact tcgggcgaat ggaaatcaag
    11341 ttttcgtcct gggccagcca atcgacg
//
LOCUS       DATNDT010000429        10681 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3274159, whole genome shotgun sequence.
ACCESSION   DATNDT010000429 DATNDT010000000
VERSION     DATNDT010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10681
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274159"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="VN788_14085"
     CDS             complement(<1..726)
                     /locus_tag="VN788_14085"
                     /inference="COORDINATES: protein motif:HMM:NF013767.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HXU21709.1"
                     /translation="MTFPSDEQTSELHDLFFESAAEILQGMNDAGLALEERPGDAEQL
                     RHVRRAVHTLKGDSAACGYRQLSELAHDLEDVLTPELAQQNAGLTAEVILTAADTFHE
                     MLAAYRSKLQPPAGGALREHIRKLLNKPEAETQDSSFEAKFDWTEYERLMIAEAFGRS
                     EAVYNIALQLDPEALLPAAALELVRKGLEGAGRILAFRPDMNAPIDELRLIQAALSSA
                     KSPDWIRQRCHIPSIVKKISIDAV"
     gene            complement(723..1631)
                     /locus_tag="VN788_14090"
     CDS             complement(723..1631)
                     /locus_tag="VN788_14090"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HXU21710.1"
                     /translation="MSTTVTGPVQLTDAELKLLQTLVYQECGMYFDERRTHFLRDRLQ
                     RRLKACQLDSFYSYYRLLTSREGKQELTALLQNLTVNETSFFRIRPQLELFQKVILEQ
                     ILHRKQERRDWSIRVWSAGCSTGQEPYSLAILICDALAYYYLRNPLPFDMPTPKPLIP
                     PPWKIEIVASDISYSALLTAQQAIYTEPQMEAVDYTCRLRYFDKVGDKYAIKPALRNL
                     VQFDFHNLKAEYLPQHNDVIFCRNVMIYFDEAEQKRLIDKFHRCLNQEGYLFVGHAES
                     LFGLTTKFHMIHQNNATVYQKIEAAQ"
     gene            complement(1662..2162)
                     /locus_tag="VN788_14095"
     CDS             complement(1662..2162)
                     /locus_tag="VN788_14095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HXU21711.1"
                     /translation="MEKELQVVGFRIGRETFGLPISIVREIVRVPEITSVPNAPDYIE
                     GVINLRGRIIPVVDLRKRFGDKAFEPSKKNRIVVVELETRAIGLIVNSASEVLRIPPS
                     DIEEPHNVFKEGEVSYITGVGKLSGRLVILLDLSRILQPGELENIEEISESTTAVPAG
                     GGRRGF"
     gene            complement(2180..4183)
                     /locus_tag="VN788_14100"
     CDS             complement(2180..4183)
                     /locus_tag="VN788_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HXU21712.1"
                     /translation="MTIGKRLYAGFGAVLAIMAFLLAINILTVIRQYNARDAVKSTLA
                     DVQCIENVRYKIIENRLSLGNYLLSGDLRDEEKTNKGISELQNLLNDGEQKANDAGLR
                     SSLVQVQSNEHDWADNFAKQMIAKRHQVDSGDATVSDLQIFYLQHDPTAQINKSTAVL
                     DQANDAVRKAQDQSNASSASATLLSAIITTVGTLIAVVLGGLIAFYTAKSIKDPLYHL
                     IEVAQRISNTGDLDQTIDVHRGDELGTLAENFNQMVLHLKEMASVSAAIAEGELSVAV
                     QPRSQQDTMAKAFAQMTQGLRQMVRQVRDGASQVASGAGQMAGASDESAKVSVQAASA
                     IDEVTSTMHEMSVNVQNVVKNTQVQASSVAETSASIDQMVTSIQRVADTAKLLVDISH
                     RSREEARSGMVTMDKATEGLNRTSQAIQSSSEIIDVLGRRADDIGKIIEVIDDLAEQT
                     NLLALNAAIEAARAGEHGLGFAVVAEEVRKLAEKSTQSTKEISELIEGIQKEAREAVE
                     NMEKSTAMVQEGLVLNKDLSVALDKISDVVSEVYKFSQEIGAATTEQSSGSAQIAKAT
                     NRLTEITQEINSSVEEQASGAQAVVRSMERMRELVQQSSSSSTELAATAEQMSKLSRA
                     LLDSMDRFAIEETRGDYKRTPARRHTDPTERHASPEYAEMARS"
     gene            complement(4234..4587)
                     /locus_tag="VN788_14105"
     CDS             complement(4234..4587)
                     /locus_tag="VN788_14105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXU21713.1"
                     /translation="MKYTLTFATLGLALLLAGSAILPPKVLAQDDASTDASKRKVRNK
                     VVPEYPPLAKQMNVTGKVKIEATIAADGHVTNTRVVGGSPLLVNAALDAIKRWRFEPA
                     PKDTTEVVEFSFSGQ"
     gene            4918..6903
                     /locus_tag="VN788_14110"
     CDS             4918..6903
                     /locus_tag="VN788_14110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU21714.1"
                     /translation="MRALKRIPEVPFRLDGSFESLLAQFSATTLELNPSLGTSEFARR
                     LTARTAEMTGARAVILVLGRLSDWEVAGFSGRGHHWELAIQRQLAAALARNARGPGAA
                     LRSGPAATLLGRDLAERLGWSNLVLVRLTESEGAPLGILCLVDAGRELSSVEKQLLEA
                     LAGHASVALENVRLFSRIERSRKQWVQDVDAISDFIVVHDRAGCVLRLNRALADLLGL
                     PPTRAVGTDIGKLRLLATPEQPGKCPFCRNAELAQEDFVHATAGRTFLVSTSRIHSGE
                     EEELRTVHVMKDITDRREAERRYRRERDFNRNILNNTQSMIMVLDTAGLVSYANRRCF
                     ESGYREADLLGHPFVNMVPSGRRRALMETLDRTLQGIAIDNFEIPLLRGNGAAGHFSI
                     SLSPMRDEQGHINSIVVVMTDITDAASLQAKLMHTEKMAALGQLVSGVAHEVNNPLAA
                     IVGFTDLLLENPGVPSEAKDELLVILQEAQRTRVIVQNLLSFARQMPAQREPVRLNSV
                     VQQTLKLRSYDFSNHGVEIAEHYDADLPLIIGDPHQLQQVFLNILNNAYDAIQEVRRP
                     GRMSISTTFQDGQIEISFRDNGPGILHPERIFDPFFTTKEVGKGTGLGLSICYGIVRA
                     HQGEIVARNNSDGKGCTFIVRLPIAARNSETEASESA"
     gene            6900..7307
                     /locus_tag="VN788_14115"
     CDS             6900..7307
                     /locus_tag="VN788_14115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU21715.1"
                     /translation="MMTLSPATPSSDPLKILVIEDEPSVASFLCTALKRRGYDVVSSP
                     SAVEGLQLLASKDFSGVISDFRTPGGISGADLRDWLLRHRPQLAARVIFITGDTASAE
                     TVALLAQTRTPCVEKPFRVRELLAMVEKTIGKP"
     gene            7304..8713
                     /locus_tag="VN788_14120"
     CDS             7304..8713
                     /locus_tag="VN788_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HXU21716.1"
                     /translation="MRDESRLSFLIVDDEQSIRRLCMTVGQGLNFACAEAETAEAALV
                     LLETAAPDIVVTDLKLPNLSGTDLLRKIKDLLPRSEVAIMTGHGSIETAVEAMRQGAY
                     DYIEKPFRVERLRQLLQRMAEKVRLVTENQFLRERITAETQLDGIVGSSAKIQDVLRM
                     VSRLKDIRTPVLITGESGTGKELVARAIHFRGPLAPMPFIAVDCGALVPTLMESELFG
                     HEKGAFTGALKSKAGLFESANGGTIFLDEIGELPLEMQAKLLRVLQEKEVRPVGSNEK
                     KAVDVRVIAATNRDLESAYRAGTFRKDLYFRLNVVTVHLPSLRERRSDIPQLVHSFLD
                     RYAPGENIQVTPLAMKSFLQYEWPGNVRELENCIARAVALGNHRTIDVEDLPPAIRGS
                     QDAGTPSEEGMELSTTALADLERITILRVFEQAGGDKNVAGKMLGISRATLYRKLKRY
                     NIPLRSGPKELAGPAESGR"
     gene            8988..9338
                     /locus_tag="VN788_14125"
     CDS             8988..9338
                     /locus_tag="VN788_14125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21717.1"
                     /translation="MSSKVEAATTLERQTCVEELAHLFPHASPVRIGVRVSTVTAEPR
                     RLQEQTVVEFGTAREVLFSSTLPLEFEDRVRLVNSDGSFDAQATVVAVRYHGGRKAVA
                     ARFVGEVGNWIIKP"
     gene            9335..>10681
                     /locus_tag="VN788_14130"
     CDS             9335..>10681
                     /locus_tag="VN788_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21718.1"
                     /translation="MTLKTSTSTMNMAGGFLQVASGPVDLASQWKEARLVYPLYAAFA
                     EQFDLLPLPYPPGELPPARPTRSVFDSDLKWLDAVDQKVLAYQMRQIPSEILNVSEES
                     LRALIHRQLRKVNKSDTDRDKIDLLLVQYFVLCASADLVHNEVTLRDVARLLQPVLGD
                     ADVTPLEWCEPLEQILEKIRNCASLRDMLEEGLLEQGRMLKESAGHLFYDPAALVAFC
                     RFNFLVRRGFIRVLHADLGAMRQAIASLEDKGVKTVDCRSAGFSAAETTAQLRHFCEN
                     WRQPFQKDYTQSSVRRAFEQLLALRADLEDALGIGRSRVKPDLSVASAVRREAPSAVS
                     SGQGQTAPPSANLAAKEIAQVPSAKILPEAFPSTMTDVSAPKHDVVGVSSQAPAAQDV
                     AADPGKCLEAIWEQLIAAPPSRGRSMSTVVLQDTKVLLSSWEVTAFINEGGQEAEDL"
BASE COUNT         2231 a         2912 c         3103 g         2435 t
ORIGIN      
        1 cactgcgtca atcgatatct ttttcacgat ggatggaatg tggcagcgct gccgaatcca
       61 atcgggcgat ttcgcactcg acaaggcggc ctgaatcaaa cggagctcgt cgatgggcgc
      121 gttcatatcg ggtcgaaagg cgagtattct gccagccccc tcaagccctt tcctgacgag
      181 ttccaacgcc gccgcaggga ggagcgcttc gggatcgagc tgcagcgcga tgttataaac
      241 ggcctcgctg cgcccgaagg cttccgcgat catcagccgt tcatattctg tccagtcgaa
      301 cttcgcctca aatgaggaat cttgcgtttc cgcttcgggc ttgttcagca gcttccggat
      361 gtgttcgcgg agcgcacctc cggcgggcgg ctgcagcttg ctccggtagg ccgcaagcat
      421 ctcgtgaaat gtgtcagcgg ctgtgaggat gacctcggcc gtgaggcccg cattttgctg
      481 tgccagctca ggagtgagta catcttccaa atcatgggca agctcgctca actgacggta
      541 gccgcacgca gcggagtcgc ccttgagtgt gtgtactgcc cggcgaacgt ggcgaagctg
      601 ttccgcgtcg cccggccgtt cttcgagggc cagacccgca tcgttcatgc cttgaagaat
      661 ttccgccgcg ctctcaaaaa agagatcgtg gagttccgag gtctgctcgt cggaagggaa
      721 agtcactgcg ccgcctcgat tttttgatat accgtcgcgt tgttctgatg aatcatgtga
      781 aatttcgtgg taaggccgaa tagactttcg gcgtgaccga cgaagagata gccttcctgg
      841 ttgagacagc gatggaattt gtcgatcagg cgcttctgtt ccgcctcgtc gaaatagatc
      901 attacgttgc ggcagaaaat tacgtcgttg tgctgcggca ggtattcggc cttcagattg
      961 tgaaaatcaa actggaccag gttcctcaag gccggcttga tcgcgtactt atcaccaacc
     1021 ttatcgaagt agcgcagccg gcaggtgtag tccacggctt ccatctgagg ttccgtgtag
     1081 atggcctgtt gagcggtaag caatgcggag taactgatgt ccgaagccac gatctcgatt
     1141 ttccagggag gaggaatcag cggtttggga gtcggcatat caaagggcaa aggattgcga
     1201 aggtagtagt aggccagcgc atcgcagatg agaatcgcca ggctataggg ttcctgaccc
     1261 gtggagcagc ctgcgctcca aacgcggatc gaccaatcgc gccgttcctg ttttctgtgc
     1321 aagatctgtt cgagaatcac cttctgaaat aattccagtt ggggccggat acggaagaag
     1381 cttgtttcat ttaccgtgag gttctggagg agtgccgtga gctcctgctt gccttcccta
     1441 ctggttagca ggcggtaata gctataaaac gagtccaact ggcacgcctt taggcggcgc
     1501 tgcaaacgat cgcggaggaa gtgtgtgcgg cgctcgtcga aatacatgcc acactcttga
     1561 tatacgagag tctgcaatag ttttagctcg gcgtcggtta attggacagg gccagtaact
     1621 gtggtggaca ttcgtccctc tggggaaatt cccctgggct gctaaaatcc tcgccgccca
     1681 ccgccggccg gaacagccgt agtggattca cttatttcct cgatattctc gagttcgccg
     1741 ggttgcaaga tgcggctgag gtccaagaga atgacgaggc gaccgctgag ctttccgact
     1801 cccgtgatgt aactgacttc gccttccttg aagacattgt gaggttcttc gatgtcggac
     1861 ggagggattc gaagaacctc cgacgcggaa ttcacgatga ggccgatggc gcgagtctcc
     1921 agttcgacga ccacaatgcg gttctttttg cttggctcga atgccttatc gccgaatcgt
     1981 tttcgcaagt ccacgacggg aatgattcgc ccgcgaagat taatgactcc ctcgatgtaa
     2041 tcgggcgcgt tcggtaccga ggtgatttca ggtacgcgaa caatctcgcg gacgattgaa
     2101 atcggcaaac cgaaggtttc gcgaccgatc cgaaagccga ctacctgaag ttctttctcc
     2161 atggcggttg tgtgccctat caggaccgag ccatttcggc atattccggc gacgcatgcc
     2221 gttccgtcgg atcagtgtga cggcgtgcag gcgtgcgttt gtagtccccg cgcgtttctt
     2281 cgatggcaaa gcggtccatc gaatcgagca gggcgcgcga aagtttagac atctgctcgg
     2341 cggtggcggc cagctcagtg gagctggacg aggactgttg aaccagctct cgcatgcgct
     2401 ccatcgagcg gacgactgct tgcgcgcccg aggcttgctc ttcaaccgac gaattgatct
     2461 cctgcgtgat ttcggttagg cggttggtgg ccttggcaat ttgagccgaa ccgctagact
     2521 gttccgtggt agctgcgccg atttcttggg aaaatttgta aacctcggac acgacatccg
     2581 agatcttgtc gagcgccacg ctcagatcct tgttgagaac caacccctcc tgaaccatcg
     2641 cggtgctttt ttccatgttc tcgacggctt cgcgagcttc cttctgaatc ccctcgatga
     2701 gttcggatat ctccttggtc gattgggttg atttctccgc gagtttgcgg acttcctccg
     2761 cgactacggc aaagccaagg ccgtgttcgc cggcgcgcgc ggcttcgatc gccgcgttga
     2821 gtgccaggag gttcgtttgt tcggcgaggt cgtcgattac ctcgatgatc ttgccgatat
     2881 cgtcggcgcg gcgccccagc acgtcgatga tttcagaaga agattgaatg gcctggctgg
     2941 tgcgattgag gccctcggtt gctttatcca tcgtaaccat gccgctgcgg gcttcctcgc
     3001 gcgagcgatg cgaaatatca accagcagct tggcggtatc ggcgacgcgc tgaatcgagg
     3061 tgaccatctg gtcgatcgag gcggaggttt cggcaacgct cgaagcttga acctgggtat
     3121 tcttgacgac gttttgcacg ttgacgctca tctcgtgcat ggtgctggtt acttcgtcga
     3181 tcgcggaagc ggcttgcacg ctgaccttgg ccgactcgtc ggaagcgccg gccatttgtc
     3241 ccgcgccgct ggcgacctgg gaagcgccgt cgcgcacctg acggaccatc tgacggagac
     3301 cttgcgtcat ctgcgcgaag gctttggcca tggtgtcctg ctgcgaacgg ggttgcacgg
     3361 ctacggataa ctcgccctcg gcaattgccg cggaaacgga ggccatctct ttaagatgga
     3421 ggaccatttg gttaaagttc tcggcgagcg tccctagctc atcaccgcga tggacatcga
     3481 tggtttggtc caaatccccg gtattgctta ttcgctgcgc cacctcgatc aggtgataca
     3541 agggatcctt gattgatttc gcggtgtaga aggcgattag gccgccgagc acaacggcaa
     3601 tcaacgtacc gacggtagtt atgatcgcgc ttaagagtgt ggcggacgcg gaggatgcat
     3661 tcgattggtc ctgggctttt cgcacggcgt cattggcctg atcgagaact gccgtggact
     3721 tattgatctg tgctgtggga tcgtgctgga ggtaaaagat ctgaaggtcc gaaacagtag
     3781 cgtctcccga atccacctga tgccgtttcg cgatcatttg cttcgcgaag ttgtcggccc
     3841 aatcgtgttc gttgctttgt acctgcacaa gggaggagcg aagccccgca tcattcgcct
     3901 tctgctcgcc gtcgttcagc agattctgaa gctcgctaat gcccttattg gttttttcct
     3961 catcgcggag atcgccgctt agaagatagt tgccgagaga aaggcgattt tcgataattt
     4021 tatagcgaac gttctcgata cactgcacgt ctgccagtgt gcttttgacg gcgtcacgtg
     4081 cgttgtattg ccggataacc gtgaggatat tgatcgcgag caaaaacgcc atgatcgcca
     4141 aaaccgctcc gaaacccgca tagagcctct taccgattgt cattttcccc cgcctccgct
     4201 cccggtccag taagcgcctt cctgaaatgg ctactattgg ccgctaaagc taaactccac
     4261 cacttcagtg gtgtctttag gagccggctc gaagcgccat ctcttgatgg catcgagcgc
     4321 ggcgtttacg agcaacgggc tgccgccaac aactcgcgtg ttcgtcacat gtccgtcagc
     4381 tgcgatggtg gcctcaatct ttaccttgcc agtgacgttc atctgcttcg ccagtggcgg
     4441 atactcgggc actactttgt tgcggacctt tcgtttagaa gcatccgtgg aagcatcgtc
     4501 ttgcgcgagc accttgggag gcagaattgc cgagcctgcc aataagagcg ccaatcctaa
     4561 tgttgcgaat gtcagcgtgt atttcatcac ttgctcctcc ccagagagaa agaccccgca
     4621 ctggaaaatg cacgggcgat gccataggcg gcggcatcga taactcgagc gcggaatttg
     4681 ctctttaccg gtaacttaat gatatggagc agcttaattg ggaaagatga cggctagtcg
     4741 aaagctggcc tcggctcacg cgtctcaagt gcttggctaa cggcgtccag tcctctcaca
     4801 ttgcgacaga gttcgcgtta cccctacagc acggtgccgg tccggacttc ttgactcaaa
     4861 atgagatgcg gccgtaacgt tcttctgcgt aggcgaagcg cgcaagcccc gacctccatg
     4921 cgtgcgctaa agcgtattcc agaagtccct ttccgtctcg atggctcctt cgagagcctg
     4981 ttggcccagt tttccgccac gacgctggaa cttaatccct cgcttggcac gtccgaattt
     5041 gcgcgccgac taaccgcgcg cactgccgaa atgactggag cgcgcgctgt aatattggtg
     5101 ctgggccggc tgtctgattg ggaagtggcg ggtttcagcg gacgcggtca tcattgggaa
     5161 cttgcaattc agcggcaact ggcggcggcc ttagctcgga acgcgcgggg cccgggagca
     5221 gcattacgct cgggacctgc ggcgactctt ttaggccgcg atctggccga aaggctaggc
     5281 tggagcaacc tggtattggt tcggctgacg gaaagcgaag gcgcgccgct aggcatcctt
     5341 tgcctggtgg acgcgggccg cgaattatca agtgttgaaa aacagctcct tgaggcattg
     5401 gcggggcacg cgtccgtggc gctcgaaaat gtaaggctct tctcgcgaat cgagcgctcg
     5461 cgaaagcaat gggtccagga cgttgatgcg atctccgatt tcatcgtggt gcacgaccga
     5521 gctggatgcg tgctgcggtt gaaccgtgca ctcgcggact tgctggggct accgccaaca
     5581 cgagcggtgg gaacggatat tggaaagtta cggctgttgg ccacacccga gcagcccggg
     5641 aaatgtcctt tttgccgcaa tgcagagtta gcacaagaag atttcgttca cgcgaccgcc
     5701 ggccgaactt ttctcgtctc gacttcacga attcattccg gtgaggagga agagctccgc
     5761 acggttcacg tcatgaagga catcacggac cggcgcgaag cggagcgccg ctaccgccgc
     5821 gaacgggatt tcaatcggaa cattctcaac aacacccaga gcatgattat ggtcctcgat
     5881 acagccggcc tggtgagcta tgccaaccga cgctgctttg aatcgggtta ccgtgaggcc
     5941 gatcttttgg gtcatccgtt tgtgaacatg gtgccctccg gacgacgtcg cgctctgatg
     6001 gagacgcttg accggaccct tcaaggaatt gcgatcgaca atttcgagat accacttctt
     6061 cgcgggaatg gcgccgcagg ccacttttcg ataagcctga gccccatgcg ggacgagcag
     6121 gggcacatca atagtatcgt cgttgttatg accgacatca cggacgcggc cagccttcag
     6181 gctaagctga tgcacaccga aaaaatggcg gcactgggcc aacttgtttc tggagttgcg
     6241 catgaggtga acaatccgtt ggctgcgatt gtggggttca ccgacctgtt gctggagaac
     6301 ccgggagttc ctagcgaagc gaaagacgaa cttttagtca tcttgcagga ggcccagcgc
     6361 acacgagtga tcgtgcagaa cttgctcagt ttcgcgcgtc aaatgccggc ccaacgagaa
     6421 ccggtgcgcc tgaattctgt agtgcagcaa actttgaaac tccgttcgta tgacttttca
     6481 aaccacggag tggagatagc cgaacactac gatgccgacc tgccgctcat cattggcgat
     6541 ccgcaccagc ttcagcaagt ttttctcaat attctgaaca acgcctatga tgcgatccag
     6601 gaggttcgac gccccggacg catgtcgatt tcgacgacct ttcaggatgg tcagatcgag
     6661 atctcttttc gagacaatgg tccgggcatc ctgcatcccg aacgcatttt tgatcctttt
     6721 ttcaccacta aggaagtggg caaaggtaca ggtttggggc tgagtatatg ctacggaata
     6781 gtccgcgctc atcaggggga aattgttgcc aggaacaact cggacggcaa aggttgcacg
     6841 tttatagtcc gcttgcccat tgcggcgcgc aattcggaga cagaggcgtc cgaaagtgca
     6901 tgatgacgtt gtccccggca actccatctt cggacccgct aaagatactc gtgattgagg
     6961 acgagccatc cgtggcctca tttctgtgca cggcgctgaa gcgacgtgga tacgacgtgg
     7021 tgtcctctcc gtcagcagtc gaaggattgc aactgctggc atcgaaggat ttttccggcg
     7081 tgatttcgga ttttcggaca cccggaggaa tcagcggagc ggacttacgc gattggctgc
     7141 tgcgtcatcg tcctcaactc gcggcccggg tgatatttat tactggcgat accgcgagcg
     7201 cggaaaccgt cgcgcttcta gcgcagactc gtactccgtg cgtggaaaaa ccgttccgtg
     7261 tacgcgagct tttggcgatg gtcgagaaga caatagggaa accatgagag atgaatcgcg
     7321 gcttagcttt ttgatcgtgg acgatgagca gagtatccgt cgcctttgta tgaccgtcgg
     7381 acaagggttg aatttcgcct gcgccgaagc agaaaccgcc gaggcggccc tggttttgct
     7441 ggaaacggcc gcgcccgata ttgtcgttac agatctgaaa ctgccgaacc tttcaggcac
     7501 agacttgctg cgaaagatca aggatctcct cccgcgcagc gaagtagcca ttatgacggg
     7561 acatggctcg attgaaaccg ccgtcgaggc catgcggcaa ggagcctatg actacattga
     7621 aaagccgttt cgtgtcgagc gacttcgcca gcttttgcag cgcatggccg agaaggttcg
     7681 cctagtcacg gaaaatcaat ttctgcgaga acggataaca gcggaaaccc agctcgatgg
     7741 aatcgtcggc tcatccgcga agatccagga cgtgcttcgg atggtctcgc gcttgaagga
     7801 tatccgcacg cctgtgctta ttaccggaga aagtggaact ggcaaggagc ttgtggcccg
     7861 cgccatccat tttcgggggc ctctggcgcc gatgccgttc attgcagtgg actgcggcgc
     7921 gttggtaccg accctaatgg aaagcgagct ctttggtcac gaaaagggtg cattcacggg
     7981 ggcgttgaag tccaaagcgg gactcttcga atcagccaat ggcggcacga tcttcctgga
     8041 cgaaatcggt gaacttccgc tggaaatgca ggcgaagctc ttgcgcgtat tgcaggaaaa
     8101 ggaagtgagg ccggtcggaa gcaatgaaaa gaaggctgtg gatgtgcgcg tgattgcggc
     8161 tacgaaccgc gatctggaat cagcgtatcg ggctggcacc tttcggaagg atttgtactt
     8221 ccggctgaat gttgtgaccg ttcatctccc gagcttgcgc gaacgtcgct ctgatattcc
     8281 tcagctcgtg cattcgttct tagaccgtta cgcacccgga gagaatattc aggtgacgcc
     8341 gctggcgatg aaaagctttc tgcaatatga atggcccggg aatgttcgtg aactcgagaa
     8401 ctgcatcgct cgcgccgtcg cccttggcaa tcatcgcact attgacgtcg aagacttgcc
     8461 ccccgcgatt cgcgggtcgc aagacgcggg gacgccgagc gaagagggaa tggaactttc
     8521 cactaccgct ctcgctgatc tcgagcggat tacgatcctg cgggttttcg aacaagcggg
     8581 cggcgataag aatgtcgcgg ggaaaatgct ggggattagt cgcgccactc tgtatcgcaa
     8641 actaaagcgt tacaacattc cacttcggtc gggaccgaag gaactggctg gccctgcaga
     8701 atccggacga tagtccatat ccgtccatct accattgttc tcttctgaaa attctcgaac
     8761 taggcgcatg agacgatccg tgtcagtgtg agaaaattcc aaggactcgc ctgtccgcat
     8821 gtttctcgaa gtgcttccaa tacagtaatt tacagctcac gaagagtcgg accctttggc
     8881 cgcgctcgtg cacttagctt gaacgcagta ttgggcattg cttcattcgc gcctcgtgat
     8941 cgcggcaccc ggggcagtga cagggcggga caaaagggag caaacacttg tcgagcaagg
     9001 tcgaagcggc aaccaccttg gagaggcaaa cgtgcgtgga ggagctagct catcttttcc
     9061 cccatgcatc tcctgtgcga atcggtgtgc gtgtatccac cgttactgcg gagccgcgtc
     9121 gattgcagga acagacggtg gtcgagttcg gcaccgcacg agaggtcctg ttcagttcta
     9181 cgttgccgct ggaatttgaa gatcgagtgc ggctcgtgaa ttctgacggt tcgtttgacg
     9241 cccaagccac tgtagtagcg gttcgctacc atgggggccg gaaagctgtg gcagcgcgtt
     9301 tcgtgggtga agtcgggaat tggatcatca agccatgact ctcaaaactt ctaccagcac
     9361 catgaatatg gccggtggtt ttctccaggt cgcgagcggg ccggtcgatt tggcatcgca
     9421 gtggaaagaa gcacggctcg tttatccgct gtacgccgcc ttcgccgaac agttcgacct
     9481 cttgccgttg ccgtatccac ctggtgaatt gccgccagcc aggcccaccc gcagcgtctt
     9541 cgactccgat ttgaaatggc tggacgcggt ggaccagaaa gtattggcct accagatgcg
     9601 gcagatccca tccgagattc tcaacgtgag tgaggagagc cttcgcgcgc tgattcatcg
     9661 tcaactgcga aaggtgaaca agtctgatac cgaccgcgat aaaatcgatt tgctgcttgt
     9721 gcagtacttc gtactttgcg cctccgccga ccttgtccac aacgaagtca cgctgcggga
     9781 tgttgcgcgg cttctccagc cggttctcgg cgacgccgat gtcacacccc tcgagtggtg
     9841 cgaacccttg gagcaaatcc tcgaaaagat acgaaactgc gccagcctgc gcgatatgtt
     9901 ggaggaaggc ctattagaac agggacgaat gctgaaggag tcggctggac atctgttcta
     9961 tgatccggcc gcactcgtcg ccttttgccg cttcaacttt cttgtccggc gtgggttcat
    10021 tcgagtgctg catgccgatt tgggcgccat gcggcaagcg attgcttccc tcgaagacaa
    10081 aggcgtgaaa acagtggatt gtcgcagcgc cggcttctca gccgcggaaa cgaccgcgca
    10141 actccggcac ttttgtgaga attggcgcca acccttccag aaggactaca cccaaagttc
    10201 cgtgcgccga gcgtttgaac agctcttagc actccgcgcg gacctcgaag acgctcttgg
    10261 tattgggcgc tccagagtca agccagattt atcggttgct tccgcagtca ggcgggaagc
    10321 gccgagcgct gtaagttcgg gccagggaca gaccgccccc ccatcggcca atttggcagc
    10381 gaaagaaatc gcgcaagtgc caagcgccaa aatcttgccg gaagcattcc cgtcaacaat
    10441 gacagatgtc tccgcgccca aacatgatgt tgtcggagtt tcttcacagg cgcctgctgc
    10501 tcaagacgtc gccgccgacc cgggaaaatg cctcgaagcg atctgggaac aattgatcgc
    10561 cgcaccgcca tcgcgcggcc ggtccatgtc tactgttgtt ttgcaagata caaaagtgct
    10621 cctttcgtcg tgggaagtga cggcatttat taacgaaggc ggccaagaag ctgaggactt
    10681 g
//
LOCUS       DATNDT010000430         3105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_398655, whole genome shotgun sequence.
ACCESSION   DATNDT010000430 DATNDT010000000
VERSION     DATNDT010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398655"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..141
                     /locus_tag="VN788_14135"
     CDS             <1..141
                     /locus_tag="VN788_14135"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010200206.1"
                     /note="catalyzes the formation of 4-aspartyl phosphate
                     from aspartate 4-semialdehyde; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HXU21719.1"
                     /translation="VPIGRLHKLNMGPEYLGAFTVGDQLLWGAAEPLRRMLGILRQHL
                     DS"
     gene            222..1193
                     /locus_tag="VN788_14140"
     CDS             222..1193
                     /locus_tag="VN788_14140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21720.1"
                     /translation="MHRKIFLTVCAAALFALPSFAQTTNLNGTWKLDNAKSSFGQFPP
                     PSSETDVINIDGSNFKQQVTSTTQQGSQSYTRACTIDGKEKTLSPDDPNAHIGRITLS
                     KIKCEWQGASLVVTETANLQGSELTDRLTFTASDDRKTMTMDSHITSATINGDRKLVY
                     DAADSSAGMAPPSNNTPGTGMAASSSGSHPSLSGVWKLNTAKSDFGQGQAPASQIDTI
                     EDNEPSVKIVEDQKGGFMGDMNITSTLSSDGKPTTSTGMGGAQVTSTAHWEGGALVVN
                     SKTSFQGSDVTIKDTFTPSADGKTLTEVAHIESSMGNFDTTSVFDKQ"
     gene            complement(1272..2246)
                     /locus_tag="VN788_14145"
     CDS             complement(1272..2246)
                     /locus_tag="VN788_14145"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HXU21721.1"
                     /translation="MELRQLRYFIAVAERLSFSKAAQHLHITVPPLSRQVRQLEEEFG
                     VELFVRNRRNVALTDAGRTLLREAKSLVNQSAHVTDCVRLAKCGEAGLVKIGIGLGLG
                     EPISRVFIEHSKQFPSVELQYRDMFSSWQNKALHDGDIDVGFLRPLVDSVHLMTEVLF
                     QENFVVHLSRTNPLAKRKALRIKDLAGETLLVPNRGGSTGLYDKTLELYAEAGVTPNI
                     VHVSLDPVPHSDTQIVLLTCHKGIFIMPDEVACRPAQGSEVVAVPLDEPNAKIDVHVA
                     WRRGEKSTTVLGLVDSAIRVYSAPPRYCTSARGEPSALNSISQAERRS"
     gene            2540..>3105
                     /locus_tag="VN788_14150"
     CDS             2540..>3105
                     /locus_tag="VN788_14150"
                     /inference="COORDINATES: protein motif:HMM:NF028519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU21722.1"
                     /translation="MKARFGSRRGVFSVLVCALALLLCVPLAQAQSAGTGALTGTVTD
                     AQGGAVAGVTVTATNIGTNQERTASTGPVGDYKFSLLPPGNYRLKFAAPGFKTSEVGN
                     VTVTTTETATVNQALEVGAVTQTVTVESTVETLQTESSTLGTTVSGNQITALPMANGN
                     YTEILSLSAGTNASVDNATSLGKGTQDI"
BASE COUNT          742 a          861 c          885 g          617 t
ORIGIN      
        1 gttcccatcg gccgccttca caagttgaat atgggaccgg aatacctcgg cgcgtttacg
       61 gtaggcgacc agttgctttg gggcgctgcc gaaccgctgc gccgcatgct tggcatcctt
      121 cggcagcacc tcgactcgta agtcggccac tggtgtataa tcccgcggct ccactgaaac
      181 aatcgattcg ggccccccgt ataagcgaag aggaggtatc tatgcaccga aagattttcc
      241 ttactgtgtg tgccgccgcg ctgtttgcgc tcccaagttt tgcgcagact accaacctga
      301 acggcacctg gaaactcgac aatgccaaga gcagcttcgg ccaatttccc ccgccgtcga
      361 gcgagacgga tgtgatcaat atcgacggtt cgaatttcaa gcagcaagta accagcacga
      421 cccagcaggg aagccagagc tacacacgcg cgtgcacaat tgacggcaag gaaaagacgc
      481 tttcacccga cgacccgaac gcgcacatcg gccgcatcac tctctcgaaa attaagtgcg
      541 aatggcaagg cgcgtcgttg gttgtcacgg aaacagccaa cctgcagggt tcggagctca
      601 ccgaccgcct gacgttcacc gcttccgatg accgcaagac catgacgatg gactcgcata
      661 ttacttcagc gacaattaac ggagatcgta agttggttta cgacgctgcg gactcgtctg
      721 cgggaatggc ccctccctca aacaatacgc ccggcaccgg catggcggct tcatcctcgg
      781 gctcccaccc cagtctcagc ggagtatgga agctgaatac ggcgaagagc gatttcgggc
      841 agggacaagc gcccgcgagc caaatcgaca ccatcgagga caatgagcct tccgtgaaaa
      901 tcgtcgaaga ccaaaaaggc ggcttcatgg gcgatatgaa catcacgagc acgctgagca
      961 gcgatggcaa gccgactact tcgactggaa tgggcggcgc gcaggtgacc agcacggccc
     1021 attgggaggg cggcgcgctt gtggtcaact cgaagacgag ctttcaggga agcgacgtga
     1081 ccatcaagga cacgttcaca ccgagcgccg atggaaaaac gcttactgaa gtggctcaca
     1141 tcgaatccag tatgggcaat ttcgatacca ccagcgtttt cgataaacaa tagttggaac
     1201 tcaaacacgc agacgcgagc tgagcgacga aagttgcgca gctcattagt ctgtggtttc
     1261 caagtaggga actagcttcg gcgttccgcc tgcgaaatcg aattcaatgc tgaaggttca
     1321 cctcgcgcgg acgtgcagta tctcggcggg gccgaataaa cgcgtatcgc ggaatccacg
     1381 agtccaagca ccgttgtcga tttttcccct cttcgccacg cgacatgcac gtcgattttg
     1441 gcatttggtt cgtcgagcgg gacggcaact acctcgctgc cctgcgccgg tcggcatgca
     1501 acttcatccg gcatgatgaa aattcccttg tgacaagtca gcaggacaat ttgagtatcg
     1561 ctgtgaggca cgggatcgag agacacgtgg acgatgttgg gtgtaacacc cgcttcggca
     1621 tagagctcga gcgttttatc gtatagcccg gtggatcctc ctcggttcgg aacgagaagg
     1681 gtctctccgg cgagatcctt gattcttaga gccttgcgct tcgctagagg atttgtcctg
     1741 ctcaggtgaa cgacgaagtt ttcctgaaac aatacttccg tcatcaaatg aacggaatcg
     1801 acaagagggc gcagaaaccc aacatcaata tcgccgtcat gcaaagcctt gttttgccag
     1861 ctggaaaaca tatcgcggta ttgcaactcg acagagggaa attgtttcga gtgttcaatg
     1921 aaaacgcggc tgatgggctc gcccaagccg aggccgatgc cgatcttcac cagtccggcc
     1981 tcgccgcact tggccaggcg tacgcaatcg gtcacgtgcg cgctctggtt taccagggac
     2041 ttggcttcgc gcaaaagggt tctgccggcg tcggtcaatg ccacgttgcg gcgattgcgc
     2101 acgaagagct cgacgccgaa ttcctcctcc agttggcgaa cctggcggct taggggaggc
     2161 actgtgatgt gcaaatgctg agcggccttt gagaaactta agcgctcagc aacagcgata
     2221 aagtatcgaa gctgccgaag ttccatgggg cccacacacc ccctcgccga gttgcttgca
     2281 ttcgttatat tccgaatcgg taacagaagc aatcaaaata agaaatggac aattgtcgca
     2341 atcctgatat tagtgtgcca ttcggaagaa tagtagtcgc aggccccaag ggatgggtgg
     2401 gcttcgtcag cggcattgat aggcattcaa tagaatgagt cgaggttttc ggtacgtacg
     2461 cgaattgtcc aaggattccg agcgggcgat agggtttcaa aacgatacta gtgaccgacg
     2521 atcaatcagg gggatgccaa tgaaggcacg attcggtagc cgtaggggtg tgttttcggt
     2581 gttggtctgc gccttggcgc tgctgctttg cgtaccgctg gcgcaggcgc aatccgctgg
     2641 cactggcgcg ttgacgggta cggtcacgga cgcccaaggc ggcgccgtgg cgggcgtaac
     2701 cgtaaccgcg actaatattg gaaccaacca ggaacgcacc gcgagcacag gccccgttgg
     2761 cgattataaa ttcagtttgc tcccaccggg caactaccgg ctgaaatttg ccgcgccggg
     2821 cttcaaaacg tccgaggtcg gaaatgtgac agtcaccacg accgagacgg caaccgtaaa
     2881 tcaggctctg gaagtcggag cagtgacgca gaccgttacg gtggagagca ccgttgagac
     2941 tctacagacg gaaagttcca cgctcggcac tacggtgtcc ggtaaccaaa taactgcact
     3001 tccgatggcg aacggcaact acacggagat tttgagcctc tcggccggca ccaacgcaag
     3061 cgtggacaat gcgacatcgc tcggtaaggg cacgcaggac atcag
//
LOCUS       DATNDT010000431         4982 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4060119, whole genome shotgun sequence.
ACCESSION   DATNDT010000431 DATNDT010000000
VERSION     DATNDT010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4060119"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..971
                     /locus_tag="VN788_14155"
     CDS             <1..971
                     /locus_tag="VN788_14155"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU21723.1"
                     /translation="RISTNTTDRATIAQLCRKHTLACAFAPGQHSPPAPAAKYSKLVL
                     NVKTEVNARERRWREEARFFDERASRNPSAPPLLDPLALGRYTNPKLRSRFNKEYRFL
                     VLGDLTGKQVLDVGCGDGANSVLLAKMGAMVTGIDISPASIEFAERRAEANGVSDRTR
                     FLCSPLESADLPAKHFDVIWGDAVLHHLIAELDFVMAQLCRYTRPGGTLLFAEPVNFS
                     PILRRLRFTIPVHTDATPDERPLEPAEIEIVRHHIPGLRIRRFSFLGRLDRLLLKDYN
                     FERSHPLRRCISDLMARADAVCLRLPLLENFCGTAVLHGKAPGQPG"
     gene            complement(959..1408)
                     /locus_tag="VN788_14160"
     CDS             complement(959..1408)
                     /locus_tag="VN788_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXU21724.1"
                     /translation="MELQDTVSDLLSHKSGELRSIAPGETVYEAVELMAQNEIGALLV
                     IAADTLLGIVSERDYARKIILKGRSSKDTLVEDIMMSPPVSVTPRHTVSECMRIMTAE
                     KLRHLPVVENDRAVGIVSIGDLVNWTIRKQEETIHQLHHYIAGNYPG"
     gene            complement(1472..>4982)
                     /locus_tag="VN788_14165"
     CDS             complement(1472..>4982)
                     /locus_tag="VN788_14165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HXU21725.1"
                     /translation="YKKDGLPGDDIRSVYRDRQGNLWIGTNGAGLARLNAKGITTFTA
                     RDGISSNRIWSTFEDSSGSLWLGTGGGGIDRFRNGQFSHFTAKEGFSGDDVWAILEDR
                     EGSLWIGSAGGGLNRLRDASFATYGAPEGLSSDIALPVYEDREGALWVGTADAGVNRM
                     KGGKVTTFTTRDGLPDNQIFSIAEDARGDHWFGTRRGLSRFSNGKFTLYNAKNGLPND
                     VVHCAYTDSKGKLWVGTRGGLSHFDGRGFITYSTKDGLANDNVLSISEDRRDGTLWVG
                     TGGGLDHLVNGHFRAFGKPDGLSNDVVWAIYGDSDGTLWLGTNGGGLNRLKNGRFTSF
                     TTRDGLLDDAIFQILDDGRGNLWLSCNRGVFEVAKSELDAFAAGKVRQIAGHAFGQAD
                     GMKSRECNGGFQPAGWRLNGGRLAFPTMKGVAIVDPGHFVVNRVAPPVLIEELIADHR
                     EVSGHGPTQLPPGKGQLEFQYTALSFIEPENIHFKYILEGFDKEWTDAGSRRVAYYTN
                     IPPGEYRFRIIASNADGVWSREEASISLTLQPHFYQTVPFDALVGLSILGLFAAIYRI
                     RVSQLRLREKKLEALVEERTKALRGSEKKFRQLAENVREVFWIMDTQSGALLYVSPAF
                     DRLWGATADSVLRDPDVWFAAVHPEDRETVAGLRRQQRTGVLLECEYRIVHGDRTLWV
                     WDRAFPVLDESGRLDRIVGVVEEITQRKEAEQVLQRSRDELEKLVGERTVELTVLNKA
                     LVEENQERRRTEEQLKKAKELAEAANLAKSEFLANMSHEIRTPMNGIIGMTGLTLGTD
                     LSSEQRDYLEVVQSSADSLLLLIDDILDFSKIEARQLTLQKIGFNLRQCLEQTLAALS
                     VKAVEKGLSLSHSVAAGVPEILLGDPSRLRQILVNLLGNAIKFTSRGSVLASVGVKDQ
                     DHSGITLEFCVADTGIGIPRDKHRLIFDAFTQVDGSSTREFGGTGLGLAISSQLVALM
                     NGEIRVESEVGQGSRFYFTARFDLPEIVERRTAEPSARLSQSQDAGSQSSPDETGLRI
                     LLVEDNRINQLFARRLLEQDGHRISVASNGREALRALEQADWQFDVALMDIQMPEMDG
                     LTATREIRRIEAASNRHLPIIALTAHALNRDRERCLEAGMDEYLAKPIQIEKLLTLLR
                     DVAAGTLRGVEETSPAAPIALNA"
BASE COUNT          999 a         1578 c         1287 g         1118 t
ORIGIN      
        1 gacgaataag taccaatacc accgaccgtg caacgattgc gcagctttgc cgaaaacaca
       61 ccctggcttg tgcgttcgcg cccggtcaac actcccctcc cgcgccagcg gccaagtatt
      121 ctaagctggt tctaaacgtg aaaactgagg tgaacgcccg cgagcggcga tggcgcgaag
      181 aggcgcgttt cttcgatgag cgcgcgagcc ggaatcccag cgccccgccg ctgctcgatc
      241 ctcttgcgtt ggggcgctac acgaacccga aactccgctc gcgatttaac aaggagtacc
      301 ggtttctagt gctcggcgac cttacaggga agcaggtcct cgacgtaggc tgcggcgatg
      361 gcgcgaattc cgtgctgctc gcgaagatgg gcgcgatggt cacgggtatc gacatctcgc
      421 ccgcttccat cgagtttgcc gagcgccgcg ccgaagcgaa cggagtttcc gatcgcacgc
      481 gctttctctg ttccccgctt gaaagcgcgg atctccctgc caaacatttc gacgtgattt
      541 ggggagatgc cgtgttacat catctcatcg ccgagttgga tttcgtgatg gcgcagcttt
      601 gccggtatac gaggcccgga ggcacactgc ttttcgccga gcccgtcaac ttcagtccca
      661 tcctcagacg gctacggttc acgatcccgg ttcacaccga cgcgacgccc gatgagcgcc
      721 cactcgaacc cgcggagatc gagatcgtcc gccaccacat tcccggtctt cgtatccgaa
      781 ggttttcgtt tctcggacgc ctcgatcgtc tgctgctcaa ggactacaac tttgaacgtt
      841 cccatcctct tcgccggtgc atctcggacc tcatggcccg cgccgacgcc gtctgtcttc
      901 gcctgccgct gctcgaaaac ttctgcggca ccgccgtgct ccacggcaaa gcgcccggtc
      961 agcctggata gttcccggcg atgtagtgat gcaactggtg aatggtctct tcctgcttgc
     1021 ggatggtcca gttcaccaga tcgccgatcg agacaatgcc cactgcgcga tcgttttcga
     1081 ccaccggaag gtgcctcagc ttctcggccg tcataatccg catgcactcg ctgactgtgt
     1141 gccgcggcgt cactgaaacc ggcggactca tcatgatgtc ctcgaccagc gtatcttttg
     1201 acgaccggcc ttttaggatg atcttgcgcg cgtaatcgcg ttccgaaacg atgcccagca
     1261 acgtatccgc ggcaatcacc aggagcgcgc cgatctcgtt ttgagccatc agttcgacag
     1321 cttcgtaaac agtttcgcca ggtgcgatgg accggagttc cccgctcttg tggctgagaa
     1381 gatcgctgac ggtatcttgc aattccatag tgcgcctctc caagaatctc gtctcgatca
     1441 taggctcgct ttatacagat tgcaagggct cttacgcatt cagcgcgatc ggagccgccg
     1501 ggcttgtctc ctccacgccg cgtaacgttc cggcggcaac atcccgcaac aaagtgagca
     1561 gtttctcgat ctggatgggc ttggccagat actcgtccat tccggcttcc aaacagcgtt
     1621 cccgatcgcg attcagggcg tgcgcggtca acgcgataat cggaagatga cggttacttg
     1681 cggcttcgat tcttcgaatc tcccgggttg ccgtcagtcc gtccatctcg ggcatctgaa
     1741 tatccatcaa tgcgacatcg aattgccagt cggcctgctc taacgctcgg agcgcctcgc
     1801 ggccgttgga cgcaacggaa atccggtggc catcttgttc cagaagccgc ctggcaaaca
     1861 actgattgat gcgattatcc tcgacgagca aaatccgtaa gccggtttca tcgggactgg
     1921 attgtgatcc ggcatcctgg gattggctta gtcgggcgga cggctcagcg gtccggcgct
     1981 ccactatttc gggaaggtcg aatctcgcgg tgaaataaaa acggctcccc tgtccgactt
     2041 cgctctccac gcggatctcg ccattcatca gcgcaaccag ttgcgaagag atcgctaatc
     2101 caagccctgt cccgccaaac tctcgcgtcg acgatccgtc gacctgtgta aacgcgtcga
     2161 aaatgagcct gtgtttgtct cgcgggatac cgatccccgt atcggcgacg caaaactcga
     2221 gcgttatgcc ggaatgatct tggtctttta ctcctacgct ggccagcacg cttccacgag
     2281 aagtaaactt gatcgcattc cctagtaagt tgacaagaat ctgccgcaag cgcgacggat
     2341 cgcctaacag aatttcgggg actcccgccg ccaccgagtg gctgagagac agacccttct
     2401 ccaccgcctt taccgacaac gccgccagag tctgttccag gcattggcgc aaattgaagc
     2461 cgatcttttg aagtgtgagc tggcgcgctt caattttcga aaaatcgagg atgtcgtcga
     2521 tcagcaagag tagcgagtcg gctgagctct gcacgacctc caaatagtcc ctctgctcgg
     2581 aactgagatc ggtcccgagc gtcaggccgg tcatcccgat aatcccattc atcggcgtcc
     2641 ggatctcatg gctcatattg gcgagaaatt cgcttttcgc caggttggcc gcttccgcta
     2701 attctttggc cttctttagc tgttcttccg tgcgcctccg ttcctggttt tcctcgacca
     2761 gcgctttatt cagcaccgtt aattcaacgg tgcgctcgcc caccagcttt tcgagctcat
     2821 cgcgcgagcg ctgcaatacc tgctccgctt ctttccgctg cgtaatctcc tcaaccactc
     2881 caacgatccg gtccaatcgg cccgattcat cgagcactgg aaacgcccgg tcccacaccc
     2941 aaagagtccg gtctccgtga acgatgcgat attcacattc gagcaatacc ccagtgcgct
     3001 gctgccggcg cagtccggca accgtttccc ggtcctccgg gtgcacggcc gcgaaccata
     3061 cgtccggatc tcgcagaact gaatcggcgg tggctcccca aagacggtcg aatgcgggac
     3121 tcacgtacag aagagcgcca ctttgagtgt ccatgatcca gaacacttcc cggacgtttt
     3181 ccgcaagctg gcgaaacttt ttctcgctcc cgcgcaaggc tttcgtccgc tcctccacta
     3241 acgcctccaa ctttttttca cgtaggcgta attggcttac gcggattcgg taaatggcgg
     3301 cgaaaagccc tagaattgac agccctacaa gcgcgtcaaa cggcactgtc tggtagaagt
     3361 gaggttgaag cgtaagcgaa atcgatgcct cttcgcggct ccacacgcca tcggcgttgc
     3421 tggcgatgat tctaaatcga tattcaccag gaggaatatt cgtgtagtaa gcgacacggc
     3481 gcgatcccgc atccgtccat tctttgtcga acccttcgag tatgtatttg aagtggatgt
     3541 tttccggttc gatgaagctt aaggccgtgt actggaattc gagctgccct ttgccgggtg
     3601 gaagctgggt cggcccatgt ccgctaactt cccggtgatc ggcaatcagc tcttcaatca
     3661 gaaccggagg cgcgactcga ttgacgacga aatggccggg atccacaatc gcaacgccct
     3721 tcatagtagg aaaagccagc cggccgccgt tcagccgcca tcccgcgggt tgaaatcctc
     3781 cgttgcactc tctgcttttc atcccgtcgg cttgtccgaa tgcatgcccg gcgatctgac
     3841 ggaccttgcc ggcggcgaag gcgtcaagtt cgctcttggc tacctcgaag acaccgcggt
     3901 tacagcttag ccataggttt ccccgcccat cgtccaaaat ctggaagatc gcatcgtcca
     3961 gcaggccatc ccgcgtcgtg aagctggtga accggccatt tttaagccgg ttaagtcccc
     4021 cgccatttgt cccgagccaa agtgtcccat ccgaatctcc gtaaatcgcc cagacaacat
     4081 cgttcgagag cccgtctggc ttcccgaagg cgcgaaaatg gccgtttacg agatgatcca
     4141 gcccgcctcc ggtccctacc caaagcgtgc cgtcccggcg gtcttcggaa atggaaagta
     4201 cattatcgtt cgcgagtccg tctttcgtgc tgtaagttat aaacccacgc ccatcgaaat
     4261 ggcttaatcc tccgcgcgtt cccacccata acttgccctt gctatccgta tatgcgcaat
     4321 ggacaacgtc gttcggtagt ccgttcttgg cgttataaag ggtaaatttc ccgttactaa
     4381 acctgctgag cccccgccgc gtaccgaacc agtgatcgcc acgcgcatcc tcggcgattg
     4441 aaaatatctg gttgtcaggg agcccatcgc gggtagtgaa ggtcgtgact ttgccgcctt
     4501 tcatacggtt gacgccggcg tcggcggtgc cgacccagag cgcgccttcc ctgtcctcgt
     4561 atacaggaag cgcgatgtcg ctcgaaaggc cctctggcgc gccgtatgtc gcaaacgacg
     4621 catctctaag cctgttcagt ccgccgcccg cgctaccaat ccacaaactg ccttcgcgat
     4681 cctccagaat cgcccagaca tcgtctcccg aaaagccctc tttcgccgtg aaatgcgaga
     4741 attgcccgtt gcggaatctg tcgattcctc cacctcccgt gcccaaccac aaggatccgg
     4801 aggaatcttc aaaagtggac cagatacgat tgctcgatat accatcccgg gccgtaaaag
     4861 tagtgatccc ttttgcattc aggcgcgcca agcccgcccc gtttgttccg atccacaaat
     4921 tcccctgccg gtcgcggtaa accgatcgaa tatcgtcgcc gggcaatcca tccttcttgt
     4981 aa
//
LOCUS       DATNDT010000432         6649 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3838705, whole genome shotgun sequence.
ACCESSION   DATNDT010000432 DATNDT010000000
VERSION     DATNDT010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6649)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6649)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6649
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838705"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..625
                     /locus_tag="VN788_14170"
     CDS             <1..625
                     /locus_tag="VN788_14170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21726.1"
                     /translation="VPRLTESRVKAIDRIGGFFLGVTRPPLEYLASCSKVSLESFELA
                     RLNEASNYRKEVQQILQGWIDTEVDARLARWILESKLAQADQRDGRRAAIAQPALPML
                     DPSFLPWDEAQAPESEPRENLREASCRALREPERGALPSADEPAPEQRRKSPGLKRAK
                     LVEKRGGERYLRGGPAPALRISKPAFAQDEENALHELEHFVQRRPAA"
     gene            1419..1637
                     /locus_tag="VN788_14175"
     CDS             1419..1637
                     /locus_tag="VN788_14175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21727.1"
                     /translation="MSLPARQSQQIGFTSFDNEVREHALAVAKTPASEGVKTHAFVVA
                     KAHAIRSRKSQKCTHSQSQVAKVDALAV"
     gene            complement(2029..2499)
                     /gene="nuoI"
                     /locus_tag="VN788_14180"
     CDS             complement(2029..2499)
                     /gene="nuoI"
                     /locus_tag="VN788_14180"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HXU21728.1"
                     /translation="MPSIVREVGETFVTLFKGFSVTLRTMFGKTTTESFPDVPPSVQA
                     RYRGIHVLQRDENGLEKCVACFLCSAACPANCIYIEAAENIESKRISAGERYATVYNI
                     DYSRCIFCGYCVEACPTDAITHGHSIELAPYDINALIYRKEQLLEPWPPRNPSS"
     gene            complement(2544..3578)
                     /locus_tag="VN788_14185"
     CDS             complement(2544..3578)
                     /locus_tag="VN788_14185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HXU21729.1"
                     /translation="MTNDEIYERYDVICIGAGPTGLACAIEAERNGLRPLVIDKGALC
                     NSLLNYPVNMNFFTTPERMEIGDIPMTISGGKPTRVEALKYYRRAVEHYAIQTRLYEK
                     VLRITGADGDFVVQTQTELGARNLYHGAKIIIATGYYDLPNYLNVPGEDLSHVSHYFK
                     EAHPYWNQEVIVIGAKNSAAEVALDLFRSGAKVTLVHRGPELGKTLKYWVKPDIENRI
                     RAGEIKAMFSTHVTRIEPGKVWVRNSGPEHAIPAAQVFAMTGYHPDFEFLEKQNIQLD
                     PQIRSPKCDPDTLETNVPGIYVAGVVVGGKKTSDIFIENGRFHGRQIIAAITGHGKID
                     EPAPVAPPGE"
     gene            complement(3723..4655)
                     /locus_tag="VN788_14190"
     CDS             complement(3723..4655)
                     /locus_tag="VN788_14190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21730.1"
                     /translation="MLRRMFPRYRPSNWQMNRGVEIPNFPLGPENTYFIPGKMPDPSR
                     LASLGPTYPSAHAPERRPEHTQLEPPPEVQQFQPQPQVRVELIRVYDEPDPASVEAQP
                     PVEPTPQPAAAIAEPTVQPVPSEPPPKHKKKYKKRPRRKARRNIEEEFALVQKGVLTP
                     VGHHERLCQVCRNDFRENIEEEFTHWHNVHDIAAHYGIEPRTIYRHAKALNLYDERDR
                     NLRFALGHIIHRAQSVRITADALVRAVHHFARISRDGRWVDPPTHVVVSPGSRVEVPP
                     PDPLPADAIDVTPSSEELPTLIDAKGRVEHDPTD"
     gene            complement(5273..>6649)
                     /locus_tag="VN788_14195"
     CDS             complement(5273..>6649)
                     /locus_tag="VN788_14195"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HXU21731.1"
                     /translation="AVLSNGTTPDYFVGHVDEMNNDWVWHQNLLLPPGTHHITVTHQG
                     KTVWSGDVKIEANKKTVVYVAKNMQKTVDWKRGEKLKDVARFKAGLASAQIAVAPTTG
                     NFSIANENINCGQTATLNWQTGETVDANINGIGKVQPSGSQTVSPHATTTYTFNATGP
                     GGTVTGTGTVTVNTKVDASLTANPAETHYRKIGEKVITQDSSTLTWQTSNADNVTLDN
                     NKVDPSGSQMVKPEPMNTAEVPEGQPARTIDENKSYTLNATNVCGGSATQNASIHIVG
                     SVEPIPTVTLQSVFYPTDYPDKRHPQVGLVRSQQLTLATLADGFKKYLEYDPDAKLSV
                     EAHADARGSKPFNQDLSERRVERIKQYLVEQGISADKVETAAYGKEQPLDRDTVKTLE
                     STNPNMPPKMRLRQTRADWLAYNRRADVILLPSGKKSAQFFPHNADDSKLIWQVPKPP
                     LKKVEADQ"
BASE COUNT         1283 a         1755 c         2111 g         1500 t
ORIGIN      
        1 cgttccccgc cttacggaat ctagggtgaa agcaattgac aggattggag gctttttcct
       61 gggcgttact cgtcccccgc tggaatattt ggcaagctgc tcgaaggtgt cgctggaatc
      121 tttcgaactg gcgcgcttga atgaggcgtc gaattatcga aaggaggtgc agcagattct
      181 gcaaggctgg attgataccg aggtagacgc gcgcctcgct cgttggattc ttgagtcgaa
      241 gctagcgcag gccgatcagc gcgacggccg gcgcgcggca atagcgcaac ctgccctgcc
      301 gatgctcgat ccctcatttc tgccgtggga tgaggcgcag gcaccggaaa gcgagccgcg
      361 agaaaacctt cgcgaagcga gttgccgcgc gctccgagag cctgagcggg gggcgctgcc
      421 gtccgccgac gagccagcac cggaacaacg ccgaaaatct ccggggctga aacgcgcgaa
      481 gcttgtcgaa aaacgcggag gcgaaagata tttacgcggc ggccctgctc cggcgcttcg
      541 catctcgaag ccggcgttcg ctcaagatga ggaaaatgcg ctgcatgagc tggaacattt
      601 tgtgcagcgc cggccggctg cgtgacgcga gaattttcag aaatttgcgg ccgagaaaga
      661 tttacagatg cgcgatcaaa ggccgggttt agccagccgc acggccaatc cctcgctcca
      721 gggtatgaac gatcgtcgcg ctgaagtacg cgtctttgca tccaccgccg cgacgtctgc
      781 cgtcgtgtgt catgcgtagc cgttccggtc gatttcgatc tcggcgcttg aacgcgcgct
      841 accagtgacg gctgcggacc ggtgattcta ccggcggatt cgcataaacg cttggcgatt
      901 gggctgtttt cgtcccaggc gggcgacctg ccgtcagaaa gctgtgacgc tgccgcgcgc
      961 tgtttgcatg agtcgtattg cgcctgcgcc ggcttcttga tgttcgaaga acggtgcact
     1021 tctttcactg ccgtcgcgta gacttttgat gaggcgacgt tggacgccgg cttttgaatg
     1081 tgattatcag tcgcttgccg attcacaata accgcccccc cttcagatcg ttccgaattt
     1141 cggtttgcag ggcgcaattt ccacggatcg gctcgcgctg cgaacttttg aatcgatgaa
     1201 cagcagattt agtgagcctg tacagcgcat ccgcgcaccc tgccaatccg actcgcggga
     1261 ctatccgacc ggcgaatgcg ttcgaaagcc agcgcgccgc gttgccgtcc aaatgccgtc
     1321 cttttcgcgc gccgcacgcg acaaatcgag gccccgcgcg gaaaccgcat gagatagcga
     1381 cgaaacgcct ccctatcgcg acgtctgagg tttgcgcctt gagcctgcct gctcgtcaat
     1441 ctcagcaaat aggtttcacg tccttcgaca acgaagttcg agagcacgcg ctcgcagtcg
     1501 caaaaacgcc cgcatccgaa ggcgtaaaaa cgcacgcatt cgtagtcgca aaagcgcacg
     1561 ccattcgcag ccgcaagtcg caaaagtgca cgcattcgca gtcgcaagtc gcaaaagtgg
     1621 acgcgctcgc agtctaaaaa cacacgccat tcgcagccac aaaggtgcac gcattcgcag
     1681 tcgcaagtcg caaaagtgga cgcattcgaa ggcgtaaaag cgcacgcatt cgcactcgca
     1741 aaacagcacg caccgccgcc catttctcgt ttccctcggc ccttgactca aacataaact
     1801 aagaattagc ggtgaggcga tggcgcgtgg agaggaaaga atcgagagtg cgcgatgcgc
     1861 gtggtatgcg gcgtcgtggg gctcgcgttc catgcggcac gccgagatgc cgatcacttt
     1921 ctcgatcaac gaacattgac cgactcgttg actcttgaag atggtcgaag tcgcgactat
     1981 gctgcgctga aattatgcag ccatggggtg gcgaaactcg cggcgatatt acgacgaggg
     2041 attacgcggg ggccacggtt cgaggagttg ttccttgcgg tagatcagcg cgttgatatc
     2101 gtatggagcg agttcgatac tgtgcccgtg agtgatcgcg tcggtggggc aggcctcgac
     2161 gcagtagccg caaaagatgc agcgcgaata atcaatattg tagacagtcg catagcgctc
     2221 accggcagag atgcgcttgg attcgatatt ttccgcggcc tcgatataga tacaattcgc
     2281 ggggcaggcg gcggagcaga gaaagcaggc tacgcacttc tccagtccat tttcgtcgcg
     2341 ctgaaggacg tggattccgc gatagcgcgc ttggacacta ggcgggacgt cggggaagct
     2401 ttccgtggta gtcttcccga acatcgttcg caaagtgacg ctgaatcctt tgaagagcgt
     2461 cacgaatgtc tcgccgacct ctcttacgat tgacggcatc gaacctcact ttagcaaagt
     2521 tgcgggaagc gggcaaattg tgatcattcg ccggggggag cgacgggagc aggctcgtcg
     2581 attttgccgt ggccagtgat cgcggcaatg atctggcggc cgtggaagcg gccgttttca
     2641 atgaagatgt ccgacgtttt tttgccgccc acgacgaccc cggcgacgta gatgcccggg
     2701 acattggttt ccaaagtatc gggatcgcac ttcggagagc ggatttgagg atccagttga
     2761 atgttctgct tctccagaaa ctcgaaatcc gggtggtagc cagtcattgc gaaaacctgc
     2821 gcggcgggga ttgcatgttc cggtcctgaa ttcctcaccc atactttgcc gggctcgatg
     2881 cgcgtgacgt gggtggagaa catggccttg atctcgccgg cgcgtatgcg attttcgatg
     2941 tcgggtttca cccaatattt cagggtcttt ccgagttcgg ggccgcggtg caccagtgtg
     3001 actttggctc cgctgcgaaa tagatcgagg gctacttcgg ccgcggaatt cttggcgccg
     3061 atgacgatca cttcctgatt ccagtatgga tgagcttcct tgaaataatg ggagacgtgg
     3121 gacaggtctt cgcccggaac attcagataa tttggaaggt cgtagtatcc cgtggcgatg
     3181 atgattttcg caccgtggta caggttgcgg gcgccgagtt ccgtttgcgt ctgaacgacg
     3241 aagtcgccgt cagcgccggt gatgcgcagg accttttcgt agaggcgcgt ttggatggcg
     3301 taatgctcga cggcgcggcg gtagtatttc agggcctcga cgcgagtagg cttgccgccg
     3361 gagatggtca tcgggatatc gccgatttcc atgcgctcgg gcgtggtgaa gaaattcatg
     3421 ttaaccggat agttgagaag cgagttgcac agcgcgcctt tgtcgattac gagggggcgc
     3481 aggccgttgc gctcagcctc gatagcgcag gcgaggccgg tggggccggc cccgatgcag
     3541 atcacgtcat atcgttcgta tatttcgtcg ttggtcacgg aacaaaccta gcgaagacgc
     3601 ccgcgagatt caagcgattg ttggcgcgaa ggggcgcacg gcggtcgtga gccgacggcg
     3661 tgcgctcgat tttatgtcct cttgcgtttg tgtcgagtag atgtgcgctc ggatgtttgt
     3721 tttcaatcag ttggatcatg ttctactcgt cccttagcgt cgattagagt tggcaattcc
     3781 tcgctcgagg gagtgacgtc gatggcgtcc gcgggaagcg gatcgggagg cggcacctcg
     3841 acccttgaac caggggagac gactacgtgg gttggcgggt cgacccagcg gccgtcccga
     3901 ctgatgcggg cgaagtggtg gacggcgcgg actagggcgt cagcagttat acggacgctc
     3961 tgggcgcggt ggatgatgtg tccgagggcg aagcggagat tacgatcgcg ttcgtcgtag
     4021 aggttgagag ccttcgcgtg gcgatagatg gtgcgcggct cgatcccata gtgcgcagcg
     4081 atatcgtgga cgttatgcca gtgagtgaac tcttcctcga tattctcgcg gaagtcgttc
     4141 ctgcaaacct ggcagagacg ttcgtgatga ccaaccgggg tgagaacgcc tttttgcacc
     4201 agggcgaatt cttcctcaat atttcggcgg gccttgcggc gaggacgttt cttatatttc
     4261 tttttgtgct taggcggagg ctcggacggg acgggttgca cggttggttc ggcgatggct
     4321 gcagctggct gcggcgttgg ttcgactggc ggctgggctt caaccgaggc cgggtcgggc
     4381 tcgtcgtaca cccggattag ctccacccgc acctgcggtt gcggctggaa ttgctgcacc
     4441 tcgggcggcg gctcgagttg ggtgtgttcc ggtctccgct caggcgcatg cgctgatggg
     4501 taggtagggc cgaggcttgc aagacgactg ggatcgggca tcttgcccgg aatgaaataa
     4561 gtgttttccg gacccaaggg aaaatttggg atttcgacac cgcggttcat ttgccagttc
     4621 gacggtctgt atcgtgggaa cattcttcgt aacattttgc tgctcctttc gtagttcgtc
     4681 ggagccttga ggctcctagc atagaatcgc agggtgcggt ggaaataaaa ggacgagttc
     4741 ctgtggactt tgggtctacg agatatggcg gtgtctcggg aagtggccat ccgatgtggg
     4801 caagttgacc tgcaaaggct cggcgagaaa gccgacgagt agtaaggacc gctctttttg
     4861 ttggagctcg aagctccctt tctctaaagg ctgcgaaaat aacagcaagt tccaaggcac
     4921 cgccgaaccc ccatggctaa agccgtgttc caaaattggc gggctattcg ctggcctcaa
     4981 aggcccgcgc gtgcgaagcg aacgaaaaag atgccgggct gaagtatccg gcgctacgtt
     5041 taagtttgag aatgcggcgc ccgtacgtgt gtggcgaatg atggagacgg agacgagcat
     5101 gtttgccacg atgtatggtg ccctacgaat gcaacagcaa agcggcgacc ggcgctacga
     5161 ggatccgtgg gtcgcacggg aaagaaaaag aaagacgcgc cggagagctg ctctcgggcg
     5221 cgtcttctgc gttcactccc tagtcctgcc gactacctac agctaattac ctttattgat
     5281 cggcttcaac cttcttgagc ggcggtttcg ggacctgcca aatcagcttg gagtcatcgg
     5341 cgttgtgtgg gaagaactgc gccgacttct tgccggaagg caggaggatc acatccgcgc
     5401 gacggttgta cgccagccaa tcggctcggg tttggcggag ccgcatcttg gggggcatgt
     5461 tgggattggt gctttcgagc gtcttcacgg tgtcgcgatc gaggggctgt tccttgccgt
     5521 aagcagcggt ctcaacctta tcggcggaga ttccctgctc gacgaggtac tgcttgatgc
     5581 gctcgacgcg acgttcgctc agatcctggt tgaacggctt cgatccccgg gcgtcagcat
     5641 gcgcttcgac cgaaagcttc gcatcgggat cgtattcgag atacttcttg aatccgtccg
     5701 cgagagtggc aagcgtgagc tgctggctgc ggacgagtcc gacttgcgga tggcgcttgt
     5761 cgggatagtc cgtaggataa aagacgctct ggagcgtgac cgttggaatc ggctcgacgc
     5821 taccgacgat gtgaatagag gcgttctggg tggcgctgcc accgcagacg ttggtcgcat
     5881 tcagcgtgta ggacttgttt tcgtcgatgg tgcgcgcggg ctggccctcg ggaacttccg
     5941 ccgtgttcat cggctcgggc ttcaccattt ggctgccgct gggatcgacc ttgttgttgt
     6001 cgagcgtgac gttatcagcg ttggaggtct gccaggtgag cgtggaggaa tcctgcgtga
     6061 ttactttttc gccgattttg cggtagtgcg tttccgcggg attggcagtg aggctggcat
     6121 cgactttggt atttacggtg acagtgccgg tgcccgtgac agttccgccc gggccggttg
     6181 cgttgaaggt ataggtggtg gtggcgtgtg gcgagacggt ttggctgccg ctgggctgaa
     6241 ccttgccgat gccgttgata ttggcgtcga cggtttcacc agtttgccag ttcaaagttg
     6301 cggtctggcc gcaattgata ttttcattcg cgatgctgaa gttgccggtg gtgggcgcca
     6361 cggcaatttg ggcactggcg agtccggcct tgaatcgcgc gacatccttg agcttttcgc
     6421 cgcgcttcca atcgaccgtc ttctgcatat tcttggccac gtacactact gtctttttgt
     6481 tggcctcgat tttcacgtca ccggaccaaa ccgtcttgcc ctggtgagtg accgtaatgt
     6541 gatgagtgcc gggcggcaga agcaggttct ggtgccacac ccagtcgtta ttcatttcgt
     6601 caacgtggcc gacgaaatag tccggcgtgg ttccgttgga gagaacagc
//
LOCUS       DATNDT010000433         6798 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3838999, whole genome shotgun sequence.
ACCESSION   DATNDT010000433 DATNDT010000000
VERSION     DATNDT010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3838999"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VN788_14200"
     CDS             complement(<1..856)
                     /locus_tag="VN788_14200"
                     /EC_number="5.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HXU21732.1"
                     /translation="MARSLVIVESPAKAKTINKYLGRNYTVKASYGHVMDLPKKNIGI
                     LLPGEENGRKKRKRKSKGKAAEKKVAKVIPVTSENIFEPTYEVIPSKLKVIGDLRKAA
                     GKAEAVYLAGDPDREGEAICAHLREILSKPRKYEKVMAAAVEEQSANGDGEKSEQSAK
                     AEKKETKAEVEARFTPPKIYRVMFNEITQKAIKAAFENPTQLNENLVDAQQARRVMDR
                     LVGYKVSPILWDKVRRGLSAGRVQTVALRVIVEREREIRAFVPKEYWTIHALLAAGEP
                     PTFEAKLTK"
     gene            complement(967..2094)
                     /gene="dprA"
                     /locus_tag="VN788_14205"
     CDS             complement(967..2094)
                     /gene="dprA"
                     /locus_tag="VN788_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HXU21733.1"
                     /translation="MHPDDYVGWLALALTPGLGARTAGKLLLEFGSPDAIFNASLTSL
                     EGQRLPAAVAQALHSRRPLSDAAKELAQTQAMGCRLVTWDEPEYPARLREIYDPPPLL
                     YVLGKVELLNRHVISVVGARRPTPYGNQMAERLSKDLADRGLVIASGLARGIDASAHR
                     GALASSCGGTIGILGCGIDVVYPKENKKIFQEMEKHGAIVSEFPMGTFPAPQNFPIRN
                     RIISGMALGVVVVEAAQYSGSLITARLGMEFGREVFAVPGNATQPASFGPNQLIKQGA
                     KLVTSWEDVVEELPTPVRAELTPVENVPSEERAALVEENLGPIERPLYALLGVDEARQ
                     IDELVELSGLSSSEVLAALFDLELKGVVRQLPGKQFLKVLL"
     gene            2356..2496
                     /locus_tag="VN788_14210"
     CDS             2356..2496
                     /locus_tag="VN788_14210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21734.1"
                     /translation="MKIGIKSWNEDLVEVLWEMAAILGCLFIIAGVCALGMLVIYIRH
                     PK"
     gene            2592..3506
                     /locus_tag="VN788_14215"
     CDS             2592..3506
                     /locus_tag="VN788_14215"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HXU21735.1"
                     /translation="MKKLRISIVEFLNTAPLVWGFTNGPLAGKYDLSFTVPSQCAEAL
                     RRGDVDVAIIPSIEYQRMDGVVALPGMAIASKREVRSLLVVAKKPIEKAKRIALDTSS
                     RSTVGLVKLLCAGYWKIAPQFAEAEPDASEMLKDADAALIIGDPALRISLKMESLAGK
                     SPSGEQCCQGDPDDMPVPGFETLFVYDVVHQWREMTGKPAVLAIWTGRRDAITPEVVS
                     DFQVSKQYGIEHVREIAEAASITLDMPPAALERYLTENIHFDLDEENLSGLELYFEMV
                     AAAGLIPRAKPLEFASAAAAHSTARRGA"
     gene            complement(3727..4041)
                     /locus_tag="VN788_14220"
     CDS             complement(3727..4041)
                     /locus_tag="VN788_14220"
                     /inference="COORDINATES: protein motif:HMM:NF013540.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HXU21736.1"
                     /translation="MAKKIKVKVFDHLRESLQDVLAFEQGREVNLRVAEVPPRPRKLQ
                     PRDIRAIRRSLNASQALFATFLNVSPNTIRSWEQGTRRPHGADLKLLSIAKKNPHALL
                     TA"
     gene            complement(4054..4266)
                     /locus_tag="VN788_14225"
     CDS             complement(4054..4266)
                     /locus_tag="VN788_14225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21737.1"
                     /translation="MIKGLGGIRKARVSNPGRGKGKRGGFRYMYLYLERRTHVHLLFV
                     FDKDEQEELNTAQRTFLKQLVDRIKD"
     gene            4676..6598
                     /locus_tag="VN788_14230"
     CDS             4676..6598
                     /locus_tag="VN788_14230"
                     /inference="COORDINATES: protein motif:HMM:NF021606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2339 domain-containing protein"
                     /protein_id="HXU21738.1"
                     /translation="MAGLQDEIRRLTEQVAALTARVYQLEQKSGVVSHAQPQQPPQPQ
                     QPMQGTGEARHEAPVPISPPTGTMLEPPSAPGVAHVVTPTPQGSPPLIPAQPALRPVS
                     AKEHPDLEKKIGQYWLNRIGIVAILIGVSYFLKLAFENNWIGPTGRIAIGLLAGIGLV
                     LWSESFRKRNYKAFSYSLKAVGIGTLYLSLWAAFQIYHLIPAAAAFAAMIVVTAATIA
                     LALSQDAELLASFALIGGFATPILLSSGQNHEVVLFSYVCLLDAGILEMTAAKPWRRL
                     LWGSFAGTMILYWGWYSAYYSQDQRALTVFFAALFAAIFAAIPLMTRYARSTRFAGPS
                     VTLTLLPLLNAAAFFLALYEMYQDQTTTLTWFALALAAIYLGLANAFAKRNTAEEAKV
                     IQMLHVAIAIAFITIAIPLKLDREWITIGWLIESAVLLFVSVKARSNFLRYLAAAALV
                     LGIARLLFFDRYNTETLVLNARFATYVAAIAVLGGIAVFGKRHATENEKPLLRIAAVG
                     ANLLALIALTLEASDYFDRMIYASGGYNYHVYQNVSLERGFTYSAIWLIYGAILMVVG
                     FRNRSAFVRWQALILIALTTGKVFIYDLSQLGGSYRIVSFIALGAVLLGISFIYQRDW
                     LKLSSRGPDKSAQETP"
     gene            6595..>6798
                     /locus_tag="VN788_14235"
     CDS             6595..>6798
                     /locus_tag="VN788_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21739.1"
                     /translation="MTRTVLGLLAALLVANSFSFFKYERPAEPAKSSGEHYVVVDETL
                     WEHARPDLGDVRIYSQQTEIPYKL"
BASE COUNT         1368 a         2018 c         1856 g         1556 t
ORIGIN      
        1 atttcgtcag cttggcttca aacgtcggcg gctcgcccgc ggccagcagc gcatggatag
       61 tccaatattc cttggggacg aacgcgcgta tctcccgctc gcgctccaca atcacccgca
      121 gcgccaccgt ctggacgcgc ccagctgaaa gcccgcgccg cactttgtcc cacaggatcg
      181 gcgaaacttt gtatcccacc aggcggtcca tcacgcgccg cgcttgctgc gcgtccacca
      241 aattctcatt cagttgtgtg ggattctcga acgcggcctt gatggccttc tgcgtgatct
      301 cgttgaacat cacccggtaa atcttcggcg gcgtgaaccg cgcctcgact tccgctttgg
      361 tttccttctt ctccgccttg gcgctttgct cggatttttc tccatcgccg ttggcgctct
      421 gttcttccac ggccgcagcc atcacctttt catacttgcg cggcttgcta agtatctcgc
      481 gcaggtgcgc gcaaatcgcc tcgccctcgc ggtcagggtc gcccgccagg tacaccgcct
      541 ccgctttccc ggcagccttt cgcaaatctc cgattacctt cagcttgctg ggaatcacct
      601 cgtaagtcgg ctcgaagatg ttttccgacg ttaccgggat aactttcgca accttctttt
      661 ctgcagcctt ccctttggat ttgcgcttcc gtttcttccg gccgttttct tctccgggca
      721 gaagtatccc gatattcttc ttcggcagat ccatcacgtg gccatacgag gccttcacgg
      781 tgtaatttct gccgaggtat ttgttgattg ttttcgcctt ggccggcgac tcgactataa
      841 ccagcgatct tgccatgcta actttcgttc ccctcgcttc gatcccgcgc tagaaatcct
      901 tctgcttaaa ggcagtcttc cccaccccgt ctgaccaccc atcctaacct gctgtccgtc
      961 tgttgattac aacaggactt tcaggaattg ctttccaggt aactggcgta ccacgccttt
     1021 gagttccaag tcaaatagcg ccgccagcac ttcggaagaa cttaggccgg aaagttcaac
     1081 caattcatcg atctgccgcg cttcatcgac tcctagtagt gcatataaag gcctctcgat
     1141 cggcccgaga ttttcctcca ccaacgctgc ccgctcctcg cttggcacat tctcaaccgg
     1201 cgtcaattcc gcccgcaccg gtgtcggcag ctcctccacc acatcctccc aggacgtgac
     1261 cagctttgcg ccctgcttaa tcagctgatt cggcccgaag ctcgcgggtt gcgtcgcatt
     1321 cccaggaacc gcaaacactt cgcggccaaa ctccattccc agccgcgccg taatcagcga
     1381 gcccgagtac tgggcggcct ccactacaac cacacctagg gccatgccgg aaataatgcg
     1441 gttgcgaatc ggaaaattct gcggtgccgg aaacgtaccc attggaaact cgctcacaat
     1501 cgcgccgtgc ttttccatct cctgaaaaat cttcttgttc tccttcggat agaccacatc
     1561 aatgccgcac ccgaggatcc cgatcgtccc tccgcatgac gaagccagcg cgcccctgtg
     1621 ggcactagca tctattccgc gggccaatcc gctcgcgatt accaagccgc gatctgccaa
     1681 gtcctttgaa agcctctctg ccatttgatt cccgtaaggc gttgggcgtc gcgcacccac
     1741 gacggaaatc acgtgccggt tcagaagttc aacttttcca aggacataca gcagcggggg
     1801 aggatcgtag atctctcgca gtcgggccgg gtactccggt tcatcccaag tgaccaagcg
     1861 gcagcccatc gcctgcgtct gggccaactc cttggcagca tcgctcagcg gtctccgcga
     1921 atgcagcgct tgcgcgaccg ccgccggcag tcgctggcct tccagagaag ttaaggacgc
     1981 gttaaaaatt gcgtccgggc ttccaaattc gagcagcagc ttcccggccg tccgtgcgcc
     2041 gagccccgga gtcaatgcca gggccagcca gcctacgtaa tcgtctggat gcattaagag
     2101 ggaaaccagc ggcagaggct aaatacgggg acgaagtcgt gtcaaggtag ggaatatacg
     2161 tacgggacgc ggccagccgc gctttcgcgc tttgtttggc gcgtcaggcg gccgcaacgg
     2221 cgcgttttgc gcccgataac gacgggaaag tgtttctaat taggaagaat aaacgcggca
     2281 aactagaagt attttttgcg agatttggcc tactcgccgg gcggggattg ttataaagtt
     2341 ccgtagaggc ttttcatgaa aattggaatc aaatcctgga acgaggatct ggtcgaagta
     2401 ctttgggaaa tggcggcgat cctcggctgc ctcttcatca ttgcgggtgt gtgcgcgctc
     2461 gggatgctgg ttatctatat tcgccatcct aaatagcact tgcctgaaag tttcttcgat
     2521 tatttgacga aacggcggct cgcgattggg gcgtgcaacg gtatgctact atctttcctc
     2581 gggctactca tttgaagaaa ctgcgcatct cgattgtcga attcttaaat acggcgccgc
     2641 tggtgtgggg ctttaccaac ggaccgcttg ccggaaaata tgatctgtcg ttcacggtcc
     2701 catcgcagtg cgccgaagcg ctgcggcgcg gagatgtgga cgtcgccatt attccctcga
     2761 tcgaatatca gcgcatggac ggcgtggtcg cgctgccggg aatggcgata gcgtctaagc
     2821 gcgaagttcg cagcttgctg gtcgttgcga aaaaaccaat cgaaaaagca aaacgcatcg
     2881 cgctggacac aagttcgcgc agcactgtcg gcttggtaaa gcttttgtgc gcgggctact
     2941 ggaaaatcgc gccgcaattt gccgaggccg agcccgacgc ttctgaaatg ctgaaagatg
     3001 cggatgccgc gctaataatc ggcgatcccg cgttgcgcat ttcgctgaaa atggaatcgc
     3061 tcgcgggaaa atcgccgagc ggcgaacagt gttgccaagg cgatcccgac gacatgccgg
     3121 tgccgggctt tgaaacgctt ttcgtttacg acgtggtcca ccaatggcgc gaaatgaccg
     3181 gaaagcccgc ggtgctcgcg atttggacgg gacgccgcga cgcaatcact cccgaagttg
     3241 tctcggattt tcaggtgtcg aaacaatatg gcatcgagca cgtgcgcgaa atcgctgaag
     3301 ccgcctcaat cacactcgac atgccgcccg ccgcgctcga gcgctacctc acagaaaata
     3361 ttcacttcga tttggacgaa gaaaatctct cgggcctcga actctatttc gaaatggtcg
     3421 ccgcggccgg cctgatcccc cgcgcgaagc cgttagaatt cgccagcgct gcggccgcac
     3481 attccaccgc ccgccgcggc gcctgagacg ttgccttcat ggctcaattc gccgttgcga
     3541 agtccgtcga cactgaagga gcggctgtag tatgccttcg agagatgtat tcttatttcg
     3601 gcaactcttc cataactttc gccgcagcag aagccttagc agttgtccgg cgactgtacc
     3661 caagaacggt acgagtgcaa ccaattcggt tgctgccgac ttcgctttgc ggcatggccc
     3721 gattggctac gcggtgagaa gcgcgtgagg atttttcttg gcgatggaca atagcttgag
     3781 gtcggctccg tggggacggc gggtgccttg ctcccaactt cgaatggtgt ttgggctaac
     3841 attcaaaaag gtcgcgaaga gggcttggct cgcgtttagc gagcggcgaa tggcgcggat
     3901 gtcacgaggc tgaagttttc gcggccgtgg aggaacctcc gctacacgaa gatttacttc
     3961 gcgtccctgc tcgaacgcca gcacatcctg aagagattcg cgcagatggt cgaacacctt
     4021 cactttgatt ttcttggcca ttgctcttct ccgttagtcc tttattcggt ccaccagctg
     4081 tttcagaaac gtgcgctgcg cggtattcag ctcttcttgc tcgtccttat cgaaaacgaa
     4141 aagcaggtga acgtgagttc ttcgttccag atacaagtac atatagcgaa agccgccgcg
     4201 cttgcccttg ccgcggcccg gattagacac acgcgccttc cggattcctc ccaggccttt
     4261 gatcatgttc cccgcgccgg attgtccaaa agagcgccct ggatattggc cagcacggaa
     4321 tcggatgcat ctttggcgag ctcatgaagc cgccgcgtaa acgccttaga ttctatgaat
     4381 accacataga ggtatatacc acagagtggt atctagagta aaggagtgac aattagccgc
     4441 catgtgtatc agatccctcg caccggcccg aaatgcgacg gggttcggga tgacagcgta
     4501 acggcaaagg caaccgctgc cgggctgaag ccgccggcgc tacacgggca acaccaaaag
     4561 caacagcgga cgcgggctag ggcgttcaga cgcggctcag aactcgtgct tggtggcgaa
     4621 actttgcgca atcgagtact tggttggggg atgacaggtg cgttagtgta cgcgcatggc
     4681 tggtctgcag gacgaaatta gacggctgac ggagcaagtt gcggcgctca cggcgcgcgt
     4741 ctatcaactt gagcaaaaga gcggcgttgt ctcacatgcc cagccgcaac aaccgcctca
     4801 gccacagcag ccgatgcaag ggactggcga agcgcgccac gaagcgccgg ttccaatttc
     4861 gccgccaacg ggaacgatgc ttgagccgcc gtccgcgccg ggcgttgctc acgtcgttac
     4921 acccacacca caaggctcgc ccccgctgat accagctcaa cctgcgcttc ggccggtctc
     4981 cgcaaaagaa catcccgatc tcgagaagaa gattggccaa tactggctca accgcattgg
     5041 catcgtcgcg attttgatcg gcgtttccta cttcctgaaa cttgcgtttg agaacaactg
     5101 gatcggcccg acggggcgga tcgcgattgg cttgctggca ggcatcgggc tggtgctatg
     5161 gagtgaaagt tttcgcaagc gcaactacaa agcattctcg tattcgctga aagcggtggg
     5221 gatcggcacg ctttatttgt ccctgtgggc ggcatttcaa atttatcact tgattcccgc
     5281 ggcggctgca tttgcggcga tgatcgtggt caccgcagcc accatcgcgc tggcgctcag
     5341 ccaagacgcg gaattgcttg caagcttcgc gctgattggc ggcttcgcca cgccgatact
     5401 gctttcgagc ggacagaacc acgaggtggt gctgttttca tatgtttgcc tgctggacgc
     5461 cggaattctg gaaatgaccg ccgcgaaacc gtggcgacga ttgctctggg gaagtttcgc
     5521 gggaacgatg attctctact ggggctggta ttcggcttac tattcgcagg atcagcgcgc
     5581 gctaacggta tttttcgcag cgctctttgc tgcgatcttc gcggcaattc cgctgatgac
     5641 tcgctacgcg cggtccaccc gatttgccgg gccgtccgtc acgctcacgt tgcttcctct
     5701 attgaatgca gcggcatttt tcctggcgtt atacgaaatg taccaggacc aaacaactac
     5761 gctcacgtgg tttgctctgg cgctagccgc aatttattta ggcctcgcca acgccttcgc
     5821 gaagcgcaac acagcggaag aagcgaaggt catccagatg cttcacgtgg cgattgccat
     5881 cgcattcatc accatcgcca ttcctttgaa gctcgaccgc gaatggatca ccattggctg
     5941 gctgattgaa tcggctgtgc tgctctttgt aagcgtaaag gcgcgaagca actttctgcg
     6001 ataccttgcg gctgcagcgc tcgttttggg aattgcgcga ttgttgtttt tcgaccgcta
     6061 caacacggaa acgctggtgc tcaatgcccg ctttgccacg tatgtggcgg ccatcgctgt
     6121 ccttggcgga attgctgttt tcggcaagcg gcacgccact gaaaacgaaa aacctttgct
     6181 gcgcatcgcc gctgtcggcg cgaacttgct ggcgctgatc gctctgaccc tagaggcatc
     6241 ggattatttc gaccgcatga tctacgcgag cgggggctac aactatcacg tttaccaaaa
     6301 cgtgtcgctc gagcgaggct tcacctactc cgcgatatgg ctgatctatg gcgcgattct
     6361 gatggtcgtg ggatttcgca accgttcggc attcgtacgc tggcaggctc tgatcctgat
     6421 cgccctcact accggcaaag ttttcatcta cgacctttcg cagcttggag gcagctaccg
     6481 gattgtgagc ttcatcgcgc tcggagccgt gctgctggga atttcgttta tctaccagcg
     6541 cgactggctg aaactttcct cgcgcggccc ggacaaatcc gcgcaggaga cgccatgacc
     6601 agaaccgtcc tgggactgct ggcggcgctc ctggtcgcga actcatttag ctttttcaag
     6661 tacgagcgcc cggcagaacc ggcaaagtcc agcggggagc attacgttgt ggtggatgag
     6721 actctttggg agcatgcgcg ccctgacttg ggcgacgtgc gcatctattc gcaacagacg
     6781 gaaattccgt acaagttg
//
LOCUS       DATNDT010000434         3956 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2394170, whole genome shotgun sequence.
ACCESSION   DATNDT010000434 DATNDT010000000
VERSION     DATNDT010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3956)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3956)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3956
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2394170"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            167..895
                     /gene="tsaB"
                     /locus_tag="VN788_14240"
     CDS             167..895
                     /gene="tsaB"
                     /locus_tag="VN788_14240"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HXU21740.1"
                     /translation="MRHVLILALDTSSPCGSLAILRDCRVIGTVSTWTDEIYSSRMFR
                     QLDFLLGEHSLNLREFNLFAVVAGPGSFTGLRVGLAAAKGWAEVYGTPIAAISGLEAI
                     AAQSHSPDSVLIAATDARRGQIYCGRYVRPMNQGDEGLALEDEERVMDPAEFADALTG
                     MSENSPLTVITPVPELVSKMLSDHKVLPASRVEEASPILAPLVGRLGYLRAQRGALDD
                     ALTLAASYVRRTDAELKWKAPSEI"
     gene            871..1350
                     /gene="rimI"
                     /locus_tag="VN788_14245"
     CDS             871..1350
                     /gene="rimI"
                     /locus_tag="VN788_14245"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HXU21741.1"
                     /translation="MESAERNLKLRPMEASDASAVVTIQVASPEIAQWTEHDYERLSS
                     IGMMAWVGEVQGVISGFLVARLITTELEILNFAVRPDARRSGIGRALIQSAFAWGKGF
                     DARSAFLEVRASNRGALQFYKRHGFEETGRRPQYYSNPVEDALVLTAPYLKPDAAAR"
     gene            1376..2512
                     /gene="thiO"
                     /locus_tag="VN788_14250"
     CDS             1376..2512
                     /gene="thiO"
                     /locus_tag="VN788_14250"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HXU21742.1"
                     /translation="MKTYDAAVIGGGIIGVTIALELAREKLSVVVLDRQQPGREASWA
                     AAGMLSPAPDSPRDVPLVPLSRQSLKLYPEFVAAIHEESGKPTGYAQEGAIEVFCGGI
                     GEAERDALVAEHRRLGLAAESISLETAREWEPSIGLSAVAVSWLPDEGTIEPRLLMDA
                     VLAAARLQRVEILSDCEATAFLRLGGRCTGVIAGGEHITAGAVVVAAGCFSAKILEPS
                     GFAASYAPTRPVRGQMLAVRSDGVRLRRVVRSHRAYLVPRADGRIVVGSTLEEAGFKK
                     QVTPAGIKHVLEGALELCPALSEAEVLETWSGLRPGTPDDLPIIGPTDLEGLLVATGH
                     YRNGILLAPVTAKLIRNCLAHRQTDFDLQAFSPLRFVRHAQVAT"
     gene            complement(2541..3368)
                     /locus_tag="VN788_14255"
     CDS             complement(2541..3368)
                     /locus_tag="VN788_14255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21743.1"
                     /translation="MSSRQTYWFRTGAILSTLVFAAGASAQQPGSASPGAAVPVASSA
                     APSNALAQAPKEAQIEVVSGTHIPLVLHNAISTRSAKLGDPVYFETIFPVMVDGHVVI
                     PAGSYVSGEITESKRPGRIKGRGELMMKLTELILPNGYMVNLNAAPNGAGTGGGETVN
                     KEGQIVGDSDKTSDVGTVIRSTEAGTGIGAGVGAAAGNIGKGVGVGSGIGAIAGLGAI
                     LLTRGPDTELPRGSTLEAVIERPIYLEADKVQFTGPGQASALAGPPNREPQRSRIPF"
     gene            3636..>3956
                     /locus_tag="VN788_14260"
     CDS             3636..>3956
                     /locus_tag="VN788_14260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21744.1"
                     /translation="MHFRLRTSLRAKTLAIIVPALIVLVAGVYFVSRMVLTTGFSHLE
                     SDLAADNLSRASNALSNEIDNLQHSAAQVASQDQMSEWIRQRDTKRIAAEFTPGAFEQ
                     LRVNF"
BASE COUNT          785 a         1144 c         1152 g          875 t
ORIGIN      
        1 tcgcctagct tgaaatccat aagtggaagt tccctccccc gaactgcata gtagaagttt
       61 aggcgtgtgt gaacgtgcgc gtcaagttat tccactggat tcctacagga tttaattggc
      121 ctaaatcgaa ggttttcgcg tcgtttggta gccgaattgc cctataatga ggcacgtgct
      181 aattcttgcc ttagacacct cttcgccttg cggaagctta gccattctac gggattgcag
      241 ggttattggc accgtttcga catggaccga tgagatttat tcctcgcgga tgttccggca
      301 gctcgatttc ttgcttggtg agcattcgtt gaatctgcgc gaattcaatc tgtttgctgt
      361 agtggcgggc ccggggtcct tcactgggct acgcgtcggt ctcgcggcgg cgaaaggctg
      421 ggcggaggta tacggcacgc cgattgcagc gattagcgga ctcgaagcta tcgcggctca
      481 atcgcattct ccagattcgg tgttaatcgc tgctaccgat gcccgccgtg gacaaatcta
      541 ttgtggacgc tatgtgcggc cgatgaatca gggtgacgaa ggcctcgcac tcgaggatga
      601 agagcgcgtc atggacccgg cggaatttgc cgacgcgctt actggtatgt cggagaactc
      661 cccgctcacc gtgataactc ctgtgcctga gcttgtgtcc aaaatgctct ctgatcataa
      721 ggtcttgcca gccagccggg tcgaagaggc ttcgccgatt ctagctccgc tcgtgggacg
      781 gcttggctat cttcgcgcgc aacgcggcgc tctggacgat gcgctcaccc ttgccgccag
      841 ttacgtccgg cgtaccgacg ctgagctaaa atggaaagcg ccgagcgaaa tttaaagctg
      901 cggcccatgg aagcgagcga cgccagcgct gtcgtcacga tccaggtggc gtctccggaa
      961 atcgcccaat ggacagagca cgattacgag aggctttctt ccatcggaat gatggcatgg
     1021 gtgggagaag tgcaaggcgt tatctctggc tttctggtcg cacgattaat cacgactgaa
     1081 cttgaaatac taaactttgc agttcggcca gatgcgcgcc gtagcggcat tggccgcgct
     1141 cttatccaat ccgcatttgc gtggggcaag gggttcgacg cgaggagtgc atttcttgaa
     1201 gtccgcgcct cgaatcgagg cgccctccaa ttctacaaac ggcatggatt cgaagagacc
     1261 ggacgccggc ctcagtacta ctccaacccc gtcgaagatg cgctggtgct caccgcgccg
     1321 tatcttaaac ccgatgcggc agctcgatga gatcccttgc tgctagaatg atcgggtgaa
     1381 aacgtacgac gctgcagtca tcggtggcgg gatcatcggc gtaacgattg ccttagaact
     1441 cgcccgagag aagctgtcag tggtggtgct ggatcgccag cagccgggac gcgaggcgtc
     1501 ctgggccgcc gccggaatgc tctcgccggc gcccgattcg ccccgcgacg tgccactcgt
     1561 cccgttgtca cgccaaagtc tgaagctcta tccagagttt gtggccgcca ttcacgaaga
     1621 gtcgggcaaa ccaaccggat atgcgcagga gggggcgatt gaggtctttt gcggcggaat
     1681 cggcgaggcg gagcgtgacg ctttggtggc cgaacaccga cgcctggggc tcgccgccga
     1741 gtcaatttct ctcgaaacag caagagaatg ggaaccatcc attggactat cagcggttgc
     1801 ggtatcgtgg ctgccggacg aaggcaccat tgagcctcgg ctgctaatgg atgcggtact
     1861 cgccgccgca cggcttcagc gagttgaaat tctctcggat tgcgaagcga cggcgtttct
     1921 gcgactcggc ggtcgttgca ccggagtgat cgccggcggc gaacatatta ccgcgggcgc
     1981 tgtggttgtc gccgcaggct gtttttcggc gaaaattctg gagccgagcg gctttgccgc
     2041 ctcttatgct cctactcgtc ctgtgcgcgg gcaaatgctc gcagtgcgat ccgacggagt
     2101 acgacttcgg cgcgttgtgc gctcgcatcg agcctatctg gtgcctcgtg cggatgggcg
     2161 gatcgtcgtg ggcagcacgc tcgaagaagc aggtttcaag aagcaagtga cgccagcggg
     2221 cataaaacat gtgctggaag gagcgctgga gctatgcccg gctctctccg aggccgaagt
     2281 gctggaaacg tggtccggtt tgcgtccggg tacccccgat gaccttccga ttatcgggcc
     2341 cactgatttg gaagggttgc tcgtcgccac gggccactat cgcaatggaa ttcttctagc
     2401 cccagtcacc gcaaaactta tacggaactg cctggcgcac cggcagaccg attttgattt
     2461 gcaagcgttc tcccctctac gattcgtgcg gcacgctcag gtcgcaacat aggattcggc
     2521 cgcgaaattt ttcgatacct ttagaacggg attcggcttc gctgaggctc gcgattcggc
     2581 gggcctgcga gtgcagaggc ctgaccaggc ccagtgaatt gcaccttatc agcttccaaa
     2641 taaatcggtc gttcgattac agcctccaga gtcgagccac ggggtaattc ggtatccggg
     2701 ccacgtgtaa gcaagatcgc tcccagtcca gcaatcgcgc cgatgcccga gccgacccca
     2761 actcccttgc caatatttcc ggcggcagct ccaaccccgg cgccaatgcc cgtgccggct
     2821 tcggtggatc gtatgaccgt gcccacatcc gaggttttgt cgctgtcccc gacgatttga
     2881 ccttccttat tcacggtctc cccgccgccg gtgcctgccc cattaggagc ggcgtttaga
     2941 ttcaccatgt agccatttgg gagaatcagc tcagtgagct tcatcatcaa ttctccgcgg
     3001 cccttgatcc ttccgggtcg cttcgattca gtaatttcgc cacttacgta ggaaccagcg
     3061 ggaattacga catgtccatc aaccatcacg gggaaaatcg tctcgaagta aacgggatcg
     3121 ccaagcttag cgctgcgggt cgaaattgcg ttgtgcaaga ccagcgggat atgagtgcca
     3181 gataccactt ctatttgcgc ttccttgggg gcttgcgcca acgcattgct aggggcggcg
     3241 gacgaagcta ccggcacagc ggcccccggc gatgcggaac ccggttgctg agcgctcgcc
     3301 ccggcagcaa acacgagggt ggagaggatc gcgccggttc gaaaccaata agtctgacgg
     3361 ctgctcatga atcgtcctcc gacgactgta tttcgaaaga tgcgtcgccg aattgcgcgg
     3421 atgcccgaag gttcgttcgc accattctgg cagcgatgga atttatcatc agtctttcgg
     3481 cgcacaccgc gcaaatctcc ggtcccctaa gcgggattgt ccgtgttcca tcctacagta
     3541 cacgactagg cacgcgcatg cagcaccgac gctaagggct gcgtccggcc cgttatactg
     3601 ggcgcgggtc acctacagga accggcgccg cctccatgca ctttcgattg cgaacatctc
     3661 tgcgtgcaaa gaccttagcc atcattgttc cagcgcttat tgtcctggtg gccggggtct
     3721 attttgtatc acgtatggtg ctgacgacgg gattttctca tctggagagc gaccttgcgg
     3781 cagacaacct ctcccgcgct tccaacgcgc tttcgaacga gattgacaat ctccagcact
     3841 ccgctgcgca agtagcctcg caggaccaga tgtccgaatg gatccgccaa agagatacga
     3901 agcgtattgc agccgagttt actcccggcg ctttcgaaca gcttcgcgta aatttc
//
LOCUS       DATNDT010000435         4376 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3550920, whole genome shotgun sequence.
ACCESSION   DATNDT010000435 DATNDT010000000
VERSION     DATNDT010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3550920"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            430..1029
                     /locus_tag="VN788_14265"
     CDS             430..1029
                     /locus_tag="VN788_14265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013653221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU21745.1"
                     /translation="MNDEMIERLTIREMIDNWILWRDALMWKEFRSLWHDDGRMMATW
                     TQGTADEFIEMSKQGFAKGVRILHFQGGSSIEVKGNRALSQTKMKIDQRAPVHDVMCD
                     VVCTGRFVDFWEKRAGRWGLVLRQPIYEKDRMDPIDPAAKLNLDQKLLMSFREGYRHL
                     AYLQTQVGYKIKMDMPGLTGPEVEALYARGKSWLAGQPL"
     gene            1129..2055
                     /locus_tag="VN788_14270"
     CDS             1129..2055
                     /locus_tag="VN788_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014515692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6282 family protein"
                     /protein_id="HXU21746.1"
                     /translation="MAVEAVAAPIRVTDAAWQAIEGGYDLQVHVSPDVIPRRVDDLDL
                     AKEFLAHKLKGFVLKSHYAPTYERAKVVTKANPGIQAFGAITLNHSVGGLNPVALEIA
                     GRSGNKIVWMPTVDAANETAGRSDGPTDKLPFWAKIQRELSATGISPAPLTVVGTDGK
                     VTPNARRCLELIGKHDMILATGHLSKKEIHALVKTAKEMGLKRVLVTHAEFPSQNMTA
                     QEQVEVADLGAMIEHCFTTMYTNKAPWDAYFDSVRKVGPERVILSTDLGQTINPPIAE
                     GFAMFAQKMLDAGFSAPDVRRMAVTNPTALVS"
     gene            complement(2060..3670)
                     /locus_tag="VN788_14275"
     CDS             complement(2060..3670)
                     /locus_tag="VN788_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012710504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HXU21747.1"
                     /translation="MFEQSLNPTGHLWLTILAALTPLVALILMLAVFRITAWLASTLA
                     GMLTIAVGIVVWHAPLANTLKSYLFGGLTGIWSIDWITFWALIIFNTLVLTGSFGRFR
                     DWMVHHATADIRIQTIMLAWAFGALLEGLVGFGYPWAVVVPILVGLGIADLEAIRVAA
                     LANNAPVSYGALGIPIIGLAAVTGLPLMALSRSIGNVVAILALLPPWVLIYLVSGWEG
                     VAEAWPLAIVGSLSYIAGQWPVAHYLGPYLPDISGSLVSFWLLFLFVKVWQPKTIRGF
                     GGVPLAPAAAISNGSEPVSGREAFSAWMPYIVLILVVVAWTGPWSHLPAISWFKLSVA
                     TKSSVTGKALASTFNFAPLIGGTSILASWLVIVTILRPGAQILKQVFIKTFKQMWGAL
                     LVAFFIFALAYIFVYTGMANSLAYGFSKMGVFFVVVAPILGWIGVALSGSNTSTNAMF
                     GPVQMVTGRLLNLPVLLLPSLNSVGAEVGKPIAPQTASVGVSTSKLVRSEGQVIRHNM
                     GWTLIVLAYLILIAVVIYHFFPNWMSLE"
     gene            4053..>4376
                     /locus_tag="VN788_14280"
     CDS             4053..>4376
                     /locus_tag="VN788_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017743860.1"
                     /note="EngC; RsgA; CpgA; circularly permuted GTPase;
                     ribosome small subunit-dependent GTPase A; has the pattern
                     G4-G1-G3 as opposed to other GTPases; interacts strongly
                     with 30S ribosome which stimulates GTPase activity;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase"
                     /protein_id="HXU21748.1"
                     /translation="MFRLQDLGWESFFANQQTEEEIRNFIAARVAEENRGFYRLYSER
                     GELWAELPGKIRHNSHDRAALPAVGDWVLACERVSERRATIQRVLARRSKFSRKMPGK
                     KTEEQI"
BASE COUNT         1041 a         1251 c         1226 g          858 t
ORIGIN      
        1 gtagcgctgc tgcgcccgcc taacatgccc tgctaagtcg agccatttcc gccgaggctc
       61 cggtcgcctc gtcttcgaaa aagtccacag actcgcctgc ttgacagaat cgctcgcatc
      121 accaaacatg gaggcatggt cgaaaagacc gcactcaccg cgcagtcggg ccactcaaga
      181 gaagcgagca gcggtgtggg cctggatggc atgcgggccc agcttttcgc agagggaatc
      241 aacctcgaag tttcaccctc taatcgacac atgagccgag tagaacgtgg cgtaactcgt
      301 tgaaaatgaa gaacggtgac acattctact cgacacgaaa tccggagggc cgcttcggcc
      361 tctccgacgc tagatcctgg aatcggtgta cacttttgcg gctgtttcat acttagcgcg
      421 ggagttccaa tgaatgacga aatgatcgag cgcttgacga ttcgcgagat gattgacaac
      481 tggattctct ggcgggatgc cctgatgtgg aaggagtttc gcagcctttg gcatgacgat
      541 ggccgcatga tggccacctg gactcagggc acagccgatg aattcatcga aatgagcaaa
      601 cagggtttcg ccaagggcgt gcgcatcctg cattttcagg gcggtagctc gatagaagtg
      661 aagggcaatc gcgccctctc gcagaccaaa atgaaaattg atcagcgcgc cccagttcat
      721 gacgtaatgt gcgacgttgt ttgcaccggg cgttttgtcg atttctggga aaagcgcgca
      781 gggcgttggg gattggtttt gcgccagccg atttacgaaa aggatcgcat ggatcccatc
      841 gatcccgccg ccaaactcaa cctcgatcaa aaacttctga tgtcttttcg agaaggctat
      901 cgccacctcg cttacttgca aacgcaggtt ggttacaaaa ttaaaatgga tatgcctgga
      961 ctgaccggcc cagaggttga ggctctttac gcgcgtggca aatcttggct cgctggccag
     1021 cctctctaga cttttgcgga gggcgcgtcg gttatatagt ggaagtggcc ttttgtatcc
     1081 tgctggccgc cgtgaaatga aaattgagag cttagggaga acgccattat ggccgtggaa
     1141 gcagttgccg caccaattcg agtgacggac gccgcgtggc aagcgatcga aggcggctac
     1201 gatttgcagg ttcacgtctc gcccgatgta attccccgcc gcgtcgatga tttggatctt
     1261 gcgaaggagt ttctcgccca caagctcaag ggttttgtgc tgaagtcgca ttacgcgccg
     1321 acctacgagc gagcgaaagt ggtcaccaaa gcaaaccctg gaatccaggc ttttggggcg
     1381 atcactctga atcacagcgt cggtggtttg aatccggttg cgctcgaaat cgcgggccgc
     1441 tccggcaata aaatcgtgtg gatgcccact gtggacgcgg ccaacgaaac cgcgggccgc
     1501 tcggacggcc ctaccgacaa gctgcccttc tgggccaaaa ttcagcgcga gctttcggcc
     1561 acgggcattt cgccggcgcc cctcaccgtg gtcggcaccg acggcaaggt gactcccaat
     1621 gctcgccgct gcctcgagct catcggcaag cacgacatga tccttgctac cggccacctg
     1681 agcaaaaaag aaattcacgc gctggtgaaa accgccaagg aaatgggtct gaagcgagtg
     1741 ctcgtcaccc atgccgaatt cccatcgcag aatatgaccg cgcaggagca agtagaagtg
     1801 gccgatctgg gcgcgatgat cgagcactgt ttcactacga tgtacacgaa caaagctccc
     1861 tgggatgcct atttcgatag cgtccgcaag gttggtccag agcgcgtaat cctctcaacc
     1921 gatcttggcc agacaattaa tccgcctatc gcggagggtt ttgcgatgtt cgcgcagaag
     1981 atgctcgatg cgggtttcag cgcgccggac gttcgccgca tggccgtcac caacccgacg
     2041 gctctcgtct cgtagccgtt tattcgagcg acatccagtt cggaaaaaag tggtagatga
     2101 ccacggcgat caggatgaga tacgcgagaa caatcagggt ccatcccata ttgtgacgaa
     2161 tcacctggcc ctcgctgcgc accagtttac tcgttgagac gccgacgctg gccgtttgcg
     2221 gcgcaatagg cttgcccact tcagctccca ctgaattgag agacggaagc agcagcactg
     2281 gcaagttcaa gagccggcct gtcaccatct gcactggccc gaacattgca ttggtggaag
     2341 tattgctccc cgagagcgcc accccgatcc agccaaggat cggcgcaacc accacaaaga
     2401 aaactcccat cttcgagaag ccgtaggcca gcgaattcgc cattccggtg taaacgaaaa
     2461 tgtacgccag cgcgaaaata aaaaacgcga ccagcagcgc gccccacatt tgcttgaatg
     2521 ttttgatgaa aacctgcttc aagatttgag cgccgggccg caggatcgtc acgatcacca
     2581 gccaggaagc cagtatggaa gtcccgccaa tcagtggagc gaagttgaac gtggaagcga
     2641 gtgcttttcc ggtgaccgaa gatttggtgg cgaccgataa tttgaaccaa ctgatagccg
     2701 gaaggtgcga ccatggcccc gtccacgcaa ccacaacgag aatgagcacg atgtaaggca
     2761 tccacgcgga aaacgcttcg cgcccggaaa ccggttcaga gccgtttgag atagcagccg
     2821 cgggcgccaa gggtaccccg ccaaaaccgc ggatcgtttt cggttgccac actttcacga
     2881 atagaaacag cagccagaac gaaacgagcg agccgctaat gtccggaaga tacggaccca
     2941 gataatgcgc caccggccac tgcccggcga tgtaggaaag cgagccgaca atcgccagcg
     3001 gccatgcctc agccactccc tcccagccgc ttaccagata gatgagcacc cacggcggca
     3061 gcaacgcgag gatcgcaacc acattgccga tcgaccgtga tagcgccatc aatggcaggc
     3121 ccgtgacggc tgcgaggcca atgatgggga tgccgagcgc tccgtaagag acgggcgcat
     3181 tattggccag cgctgccact cggatggcct cgagatcagc gattcccagt cccaccaaaa
     3241 taggcaccac cacggcccag gggtagccaa atcccaccag tccctccaac agcgcgccga
     3301 aagcccatgc cagcatgatg gtctggattc gaatgtcggc tgtagcgtga tgaaccatcc
     3361 aatccctaaa ccgtccaaaa gatcccgtga ggacgagcgt gttaaaaata atcagcgccc
     3421 agaacgtgat ccaatcgatt gaccaaatcc ccgtaagtcc gccgaacagg taggatttca
     3481 gcgtgtttgc aagcggggcg tgccacacca cgatgcctac tgcaatcgtg agcattcccg
     3541 ccaaggtcga ggcgagccat gcggtgatac ggaagacggc gagcatcaga atcaacgcaa
     3601 ccagcggcgt caatgcagcc aggatcgtca accagagatg gccggtcgga ttgaggcttt
     3661 gctcaaacat cgcggttcca gactttgaac tgaatgtcct gatttgggca atgaaacgag
     3721 aaacgccgcc ctctatacct catatcaaag ccataactgt caacggggat ttgaccttcg
     3781 gtgcgacgga tagttcgaca aattgttccg cagaatatcc ccgagatttg ctacgatggg
     3841 cttcagccgt tcattaccgc ggccaatcca cttttggagg cgcctgtgac tgtcgtttct
     3901 caccgctggg gtgagtctgc ctccgagccc ctagcctaag ccgcggttcg gatctcgtag
     3961 tctctccctg cgactctgac acttgcttat tggtgtgttc acacccaaca gccgggcgcg
     4021 tttgcgtccg catgagtcag aggagactgg aaatgtttcg attgcaggat ttgggttggg
     4081 agtcgttttt cgcgaatcaa cagaccgaag aagagatcag gaactttatc gccgcgcgcg
     4141 tcgctgaaga aaatcgcggt ttctaccgtc tctattctga acgtggcgaa ctgtgggcag
     4201 agcttcctgg aaagattcgt cacaattcgc acgatcgcgc ggcgcttccc gctgtaggag
     4261 actgggtgct ggcgtgcgag cgcgtcagcg agcgccgcgc gacgatacag cgcgtgctcg
     4321 cccggcgaag caagttctct cgcaaaatgc ccggcaaaaa gacagaagag cagatc
//
LOCUS       DATNDT010000436         6109 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3274980, whole genome shotgun sequence.
ACCESSION   DATNDT010000436 DATNDT010000000
VERSION     DATNDT010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6109)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6109)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6109
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3274980"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..855)
                     /locus_tag="VN788_14285"
     CDS             complement(<1..855)
                     /locus_tag="VN788_14285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020716522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21749.1"
                     /translation="MPPYNLALSLFSDGDPLNSYWRHLVDPTFRGLYQANAFDLAIMI
                     PYFLVMVVLAMYGIHRYHLVYNFYKNRKNIPGPPPGVVAWPKVTVQLPIYNERYVIER
                     LIETVAQFDYPRDLLDIQVLDDSTDQTQQVASDCVERHRALGVPITYIHRDNREGFKA
                     GALQEGLKTATGEFVAIFDADFIPPADFLRRTVPYFADSKLAMVQTRWSYVNRNYSTL
                     TEVEAIMLDGHFAIEHSSRYRSGRFFNFNGTAGIWRRAAIEDAGGWQHDTLTEDTDLS
                     YRAQIRRWK"
     gene            complement(1000..1359)
                     /locus_tag="VN788_14290"
     CDS             complement(1000..1359)
                     /locus_tag="VN788_14290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU21750.1"
                     /translation="MPLDSAAVPTSILTLETRPWDGAMIVQCKGRLTVEHSEALKNHV
                     RSLLPATRRIIIDLQEITRMDSSGLGAIVGLYISAKKCGCEMILINYNKSIKNLLGLT
                     NLLSVFESCAQSGMRLP"
     gene            1557..1784
                     /locus_tag="VN788_14295"
     CDS             1557..1784
                     /locus_tag="VN788_14295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21751.1"
                     /translation="MAEIPEAFVHVLLFECSSCGCPVPAAVTAGNRNVEEVDAGSVEL
                     KCSCGWSGKALGIEARKHWVHNWPTDVLDPY"
     gene            1850..2254
                     /locus_tag="VN788_14300"
     CDS             1850..2254
                     /locus_tag="VN788_14300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU21752.1"
                     /translation="MAGFKGCRRIVNERILLVDDHEILRKGIRYLLTPAWEICGEASN
                     GQEAVQKTVELSPDLVLMDISMPVMNGIEATREIRRLGLPAKIVILTMHDSVEIAKRA
                     KEAGANECLVKACGSDRLLEAIQGVLDGKPTA"
     gene            complement(2343..3062)
                     /locus_tag="VN788_14305"
     CDS             complement(2343..3062)
                     /locus_tag="VN788_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21753.1"
                     /translation="MNRRAITLSVAVLLAAALVPTAQAQRQFAFARTSGTRAAPPFSQ
                     RGVRGRGFARHHRARRFPGSFYAPYFYPDYDYGYDSELQTIEAPPPQIVEQVAQPAAP
                     APVSPPLDSVVIELQGDHWVRVTNYGQSQTDGPSSQAETDRASNPQTGVPRRVQAPES
                     ITEVPAAVLVFRDGRREEIGKYVVVGSTLYAGADYWSSGSWTRKVQLRDLDVPATLKL
                     NQERDAKFTLPSAPNEVMIRP"
     gene            3214..5400
                     /locus_tag="VN788_14310"
     CDS             3214..5400
                     /locus_tag="VN788_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXU21754.1"
                     /translation="MIGQTISHYRILEKLGIGGMGVVYEAEDTRLGRKVALKFLPDST
                     LADQQSLDRFQREARAASALNHPNICTIYDVGEHAGRPFIAMELLEGDTLKHRVTGEP
                     MKIEEILEFGVAIADALEAAHAKGIVHRDIKPANIFITKRGQAKVLDFGLAKLASQRQ
                     RVGEAVGITSQATVGAASDHLTSPGTAIGTVAYMSPEQARGEEVDSRTDLFSLGVVLY
                     EMATGRLAFAGATSAVIFDAILNRTPPSVLRLNPRLPPEIGAVIETALEKDRKLRYQT
                     AARMHADLERLRRQLGSGGTAAAKPAEKSVAVLYFENPGGSKEDEYFRDGITEDIITE
                     LSRIKDIWVLTRSAVMAYRDKTATAPEVGEQLNASYVLEGSLRRAGNRLRITARLVET
                     KTARSVWAERYDRQIEDVFAIQDEIAQSIAKALKVMLTEQEKRAIEKAPTADVQAYDY
                     YLRGRQFFHQFRRKSFDYARQMFARAIVIDPNYARAYAGVADCCSHLYMYWGASTEDL
                     KEAESASRRAVQLDPELAEAHVSRGLALLMTKKYEEAHKEFETAIELNPKLFDAYYFF
                     ARACFQQGKSEEAAQLFEKACVANPEDYQAPVLLAQTYVSLGRSLEGEATRQRSLRII
                     EKHLEFHPEDVRALYLGTAALIQAGQRDRALEWTQRTLAIDSEDPAVLYNIACTYAML
                     DMTEESITCLEKALRQGEWYKAWAEHDSDLDSIRSHPRFQALLKAI"
     gene            complement(5471..5547)
                     /locus_tag="VN788_14315"
     tRNA            complement(5471..5547)
                     /locus_tag="VN788_14315"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5511..5513),aa:Arg,seq:cct)
BASE COUNT         1398 a         1587 c         1878 g         1246 t
ORIGIN      
        1 tttccagcgg cgaatctggg cgcgatagga gaggtccgtg tcttcggtga gagtgtcgtg
       61 ctgccagccg ccggcatctt cgatggcagc gcggcgccaa atgcccgcgg tgccattgaa
      121 attgaaaaat cggccgctgc ggtagcggga cgaatgctcg atggcgaaat gtccgtccag
      181 catgatggct tcgacttcgg tgagcgtgga gtaattgcga tttacgtagc tccagcgagt
      241 ctggaccatg gcgagcttgg aatcggcgaa atagggaacg gtgcggcgga gaaaatcggc
      301 gggcgggatg aagtccgcgt cgaaaatggc gacgaattcg ccggttgcgg ttttcaatcc
      361 ttcctgcaat gcgccagctt tgaagccttc gcgattgtcg cgatggatgt aggtgattgg
      421 cacgccgaga gcgcggtggc gctcgacgca atcgctcgcg acctgctgag tctgatcggt
      481 ggaatcatcg agaacctgaa tatcgaggag atcgcgcggg taatcgaatt gcgcgaccgt
      541 ttcgatgagg cgctcgatca cataacgctc attgtagatc gggagttgga ccgtgacctt
      601 aggccaggcg acgacgcccg gcggcgggcc gggaatgttt ttgcgattct tgtagaagtt
      661 gtaaaccagg tgatagcgat gaattccgta catcgccaga accaccatca ccaggaaata
      721 cggaatcatg atggcgagat cgaacgcgtt ggcctggtaa agcccgcgaa aagtgggatc
      781 gacgagatgc cgccaataag aattaagcgg atcgccgtca ctaaacaggg aaagcgccaa
      841 attgtatgga ggcatcacaa ggtgtttact cgactaaccg ctgggcaatc acgattatac
      901 ggaggtcgag agcagtgtca acggttggta tccgacggca gcgattcaaa ctttcgatga
      961 gggagcgagc aacttttaag ccggcagcga gttcgaagtt tagggcaacc gcatgccgga
     1021 ttgcgcgcag gattcgaaca cggagagcag gttggtgaga ccgagaagat ttttgatcga
     1081 cttgttgtag ttgatcaaga tcatctcgca gccgcacttt tttgccgaga tgtagaggcc
     1141 gacgatggcg ccgaggccgg aactatccat gcgggtaatt tcctgaaggt caatgattat
     1201 tcggcgggtg gcggggagca gacttcgtac atggttcttt agagcttcgg agtgctcgac
     1261 ggtcaagcgc cccttgcatt gaacgatcat ggcgccgtcc cacggccgcg tttctaaagt
     1321 caggatcgaa gttggaactg ctgctgaatc cagtggcatt tgcgcccccg ctagtttgga
     1381 agaactatat atcgaaaaag gtgggtgagg ggaagccaga ttatcaaatg caaactatgt
     1441 aaaaaggacg cgataccagc agtcttttct aacgcgcttt ctgccccgat cttcatccat
     1501 tgcggtatgt gcgcaatgcg cagcccacgc tagccttcag acagggaggc aagaaaatgg
     1561 cagagattcc tgaagcgttt gtccacgtgc ttctcttcga atgctcaagc tgcggatgcc
     1621 cggttccggc agccgtaacg gccggcaaca gaaacgtgga agaagttgac gccggctccg
     1681 tggaactgaa gtgttcttgc ggatggtcag gaaaagcatt ggggattgaa gcacgaaaac
     1741 actgggtgca taactggccg accgacgtgc tagacccgta ctgagtgacg gaagaagtaa
     1801 aacaaggcga aaaccctatt gcgccgaaac cgaatatgcg ctgtactgca tggcaggttt
     1861 taaggggtgc cggcgcatcg tgaacgaaag aatccttctg gtagacgacc acgaaatact
     1921 gcgcaaaggc atcagatacc tgttaactcc agcatgggaa atatgcgggg aagcgtcgaa
     1981 cggccaggaa gctgttcaaa agaccgtgga actcagccca gacctggttc tcatggatat
     2041 ttcgatgcct gttatgaacg ggattgaagc gacccgggaa attcgccgac tggggcttcc
     2101 ggctaaaatt gtgatcctta cgatgcacga ttcggtggag atcgcaaaac gggctaaaga
     2161 ggccggcgcg aatgagtgct tggtgaaggc ctgcggcagc gatcgacttc tcgaggcaat
     2221 tcaaggcgtt ctggacggga agcccactgc gtgaatttga tttctgatat gcgacgactt
     2281 tcgcgggtca atccttcttt tctttgagtg cccggagcag agaaaagcga cgcgggagaa
     2341 attcaggggc gaatcattac ttcattgggc gcggacggca gggtaaattt cgcatcacgc
     2401 tcttgattga gtttcaaggt tgcgggaacg tccaagtcac gtaactgaac ttttcgtgtc
     2461 cacgaaccac tgctccaata gtccgcgccg gcatacaagg ttgatcccac gacgacatat
     2521 tttccaattt cttcgcggcg gccgtcgcga aaaacgagaa ccgcggccgg aacttcagta
     2581 attgactcgg gagcctgaac gcgacgggga acacccgtct gtggattgga tgctcggtcc
     2641 gtctcggcct ggcttgatgg cccgtccgtt tgcgattgac cgtaattcgt aacgcgcacc
     2701 caatggtcgc cttgcaattc gattacgact gaatcgagtg gaggagaaac tggcgccggg
     2761 gcggctggct gggcgacttg ctcgacgatc tgcggcggag gcgcctcgat tgtttgcaat
     2821 tcggagtcgt acccataatc gtagtcaggg taaaagtatg gcgcatagaa tgagcctggg
     2881 aagcgacgcg cgcggtggtg gcgtgcgaat ccgcgtcccc tgacaccgcg ttgcgagaac
     2941 ggaggcgctg ctcgagtgcc ggaggtgcga gcgaaggcaa actgccgttg cgcttgcgcg
     3001 gttggcacaa gtgccgctgc taggagcact gcaacggata gcgtaatcgc gcgtctattc
     3061 atgcacgaat ccgtcttagc ctcgaaagcc cgctccgttt ttcctttcat gctacgtata
     3121 gtcccggcgg tcgcaggagt caagcgaagc cggcgcattt gaccccttcc aggccacagc
     3181 gtaagatgca tgttctttgt ccgcggaatc tgcatgatcg gccagaccat ctcgcattac
     3241 cgtattctcg aaaaactggg cattggcgga atgggcgttg tgtatgaggc ggaagatacg
     3301 cgcctcggac gcaaagtcgc gctcaagttt ctgccggatt caactttggc ggaccagcaa
     3361 tcgctcgatc gcttccagcg cgaagcgcgg gctgcgtcgg cgttgaacca tccgaatatt
     3421 tgcacaattt acgatgtcgg cgaacatgcg gggcggccgt tcatcgccat ggagcttctc
     3481 gaaggcgata cgctgaagca tcgagtgacg ggcgagccga tgaaaatcga ggaaattctc
     3541 gaattcggag tggcgatcgc ggatgcgctg gaagcagccc acgccaaggg cattgtgcat
     3601 cgggacatca agcctgccaa catcttcatt accaagcgcg gccaagccaa ggttttggat
     3661 ttcggccttg cgaagcttgc ttcccagcgg caacgcgtgg gagaggccgt aggcattacg
     3721 tcgcaagcca cggtgggagc cgcctcggat cacttgacga gtcccggcac ggccatcggc
     3781 acagtggcat acatgtcccc agagcaagcc cgcggcgaag aggtggattc gcggaccgat
     3841 cttttttcgc tgggcgtagt cctgtacgag atggccacgg gacggctggc gttcgccggc
     3901 gcgacctccg cggtgatttt cgacgcgatt ctgaatcgca cgccgccgtc ggtacttcgc
     3961 ttgaatcccc gcctgccgcc tgaaatcgga gcggttattg agaccgccct ggagaaggat
     4021 cgcaagttgc gataccaaac cgccgcaaga atgcatgccg acctcgaacg tctgagacgg
     4081 caactggggt cggggggaac agcggcggcc aagccagcgg aaaagtcagt tgccgtgttg
     4141 tattttgaaa atccgggcgg gtcgaaggaa gacgaatact tccgagatgg aattacggaa
     4201 gacatcatca ccgagctttc caggattaag gacatctggg tgctgacgcg ctcggcggtg
     4261 atggcttacc gcgacaagac ggcgacggcc ccggaagtgg gcgagcaact aaacgcttcg
     4321 tatgtgctcg aaggaagttt acggcgcgcg gggaatcgcc tgcgaattac ggcgcggctg
     4381 gtggagacga aaacggcgcg ctctgtgtgg gcggaacgct acgaccggca aatcgaagac
     4441 gtgttcgcca ttcaggatga gattgcgcaa agcattgcca aggcgctgaa ggtgatgctc
     4501 actgaacaag aaaagcgcgc tatcgagaag gcgcccacag cagacgtgca ggcgtatgac
     4561 tactatttgc gcgggcggca attcttccat cagtttcggc gcaaaagctt cgattacgcc
     4621 cggcagatgt tcgctcgcgc gatcgtgatc gatccgaatt acgcgcgcgc ctacgcgggc
     4681 gtggcggatt gctgctcgca cctttacatg tactggggag cgagcactga ggatttgaag
     4741 gaagcagaat cagcgagccg ccgcgctgta cagcttgatc cggaattggc ggaggcgcat
     4801 gtgtcacgag gactggcgct tttgatgacc aagaagtatg aggaagcgca caaggaattc
     4861 gagacggcca ttgagctgaa tcccaagctg ttcgacgcat attatttttt cgcgcgcgcg
     4921 tgttttcagc agggaaaatc ggaggaagcc gcgcagttgt tcgaaaaagc gtgcgtggca
     4981 aatccggagg attatcaagc gccggtgttg ctggcgcaaa cgtatgtcag cctagggcgc
     5041 agcctggaag gggaggcgac gcggcagcga tcccttcgaa tcatcgagaa acatttggag
     5101 ttccatcctg aggatgttcg cgccctgtac ctgggcactg ccgcccttat tcaagccggc
     5161 caacgagacc gcgcgctgga gtggacacaa cgaaccttgg cgatcgactc cgaagatcct
     5221 gccgtgctct acaacatcgc gtgcacgtac gcgatgctgg acatgacgga ggaatccatc
     5281 acgtgcctcg aaaaagctct ccgacaaggg gagtggtaca aagcttgggc ggaacatgat
     5341 tccgacctgg attccatacg cagccatccg cgttttcagg ccctgctgaa ggccatttaa
     5401 gagagatggc ccgccttcgc ccctttgggc tatggcgcgg cagccttcgc tccgcttcgc
     5461 gatcgaaggc tggtggggct ggggagagtc gaactcccga cacgcagttt aggaaactgc
     5521 tgctctatcc atctgagcta cagccccaca ggaggcgagt ttaccatggc gcaaatttcg
     5581 ttgtgaaaac cccgcgattc gccgcttcgg atttctgata cttgtccagc cggcagctcc
     5641 tcacccgggg ttaatgccga gcggtgccca catacgcttt gcggccaccg cttcctacag
     5701 caaatccctt agggcgacta tgggacccaa aatgcagcaa gtcgttgata ccgtgccaag
     5761 cgcgaaattc ggcataattg agcctgatcg ccagccatta agaagagcag tgcacccggc
     5821 cagaaagagc ggaactacac catgaggaaa agaatccttt tggcagtagg actacttgtg
     5881 atgttcgcct gggttccgac gtcccgcgca gacggtctac aaatcgcctg tacgggtacg
     5941 acgacttgtt ctcctggcgg cattcagacc acgacgcacc aacccgacgt ttgacgtgag
     6001 cctggttgga aacgggagtg gcaacgagag cggggagcta tggatcgcgg tcctgacccc
     6061 agttacgagc ggcgccaatt tcaccgggcc tagcaacaat accttgtgg
//
LOCUS       DATNDT010000437         6402 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_729796, whole genome shotgun sequence.
ACCESSION   DATNDT010000437 DATNDT010000000
VERSION     DATNDT010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_729796"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..5785)
                     /locus_tag="VN788_14320"
     CDS             complement(<1..5785)
                     /locus_tag="VN788_14320"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU21755.1"
                     /translation="MTDAPLADPDSSAIAVIGMAGRFPGARSLSEFWNNLRNGIESIV
                     PLSHDQLRAAGVDPIELHDPHYVKAAAVLDDIDMFDASFFGFSPKDAAIMDPQHRVFL
                     ECAWEALEHAGWSADEFAGPIGVYAGSGMNSYLIFNLLPNRQLMDSAGLFLIRQTGND
                     KDVLATRVSYQLNLTGPSMTVQTACSTSLVAVHMACQSLLAHECDMALAGGVTIEIPH
                     GNGYLYREGEILSRDGHCRAFDAQSTGTVFGSGAGIVVLRRLRDALASGDNILAVIRG
                     TAINNDGARKVGYLAPSVSGQSDVVLEALTVAGVTAESISYIETHGTGTQVGDPIEIA
                     ALTQAFRRSTQRHGFCRIGSLKTNIGHLDAAAGIAGAIKTILALRHKQIPPSLHFSKS
                     NPAIDFENSPFRVNRTLSTWETNGAPRRAGVTSLGIGGTNAHIILEEAPPTEPPGDSR
                     PWQILTISAKTATALESAAANLAEHLEASDGLNLADVSYTCHLGRKAFSHRRSVLCQT
                     REDAVVSLRSGNPKRVFTGHAVNHTGVAFLFPGQGSQYVNMARGIYETEKEFRSQVDH
                     CAELLKPQLRLDLRTVLYPPAAGLAQASDRLAQTWLTQPALFVIEFALAKLWMSWGIC
                     PDAMIGHSIGEFVAACVAGVLSLEDALFVVARRGQLMQSMEGGAMLAAAMPERDAHRF
                     VSHDLSIGAINGPQQCVFSGSTEAIGGLEDALGRDGISCRRLHTSHAFHSAAMDPILE
                     RFADVMRGVQLNRPAIPYLSNITGTWIKHEEVTDPIYWARQVRQTVRFSEGLQELLVQ
                     PSTILLETGPGKVLSGLAPCHATASHDNGIFSSIRDSRENTDDVGILLTTVARLWTSG
                     VNIKWTGFHANERRYRVPLPTYPFERQRFWIDAPKRNRSALSEVPLAGAQTTLCHYRP
                     AWKKVDLSSAAVPAGGNSNWLIFADSFGVGAEIVRRIIALGEMAITVNPGKHFAKTGH
                     SSYSINPGHRPDYEALVTDLIHSGQIPRRILHLWPLADSNDPILDRLERHQELSFHSP
                     LFLAQALGAHDEIDSIELGIISNGLHSIDGEIIQHPESALLLGPGKVISKEFSKVYSR
                     CIDIQLPVSDAGHQGIAAQLIAELSSESLDQVVAYRRDIRWTQTLELTQIDPHLDGNR
                     LRKRGVYLITGGLGGVGLVIAEYLARTVQAKLVLVGRTELPPRVDWETYQGRHSGYDP
                     VTHRIAAVQAIEKLGAEVLIVPGDVTDLAGMNRVVSEVQTRFGSIHGVVHAAGVLDDG
                     LIQFKSREDTDRVLAPKVRGTLVLDAALGNAELDFFVLFSSISCWSAPVGQVDYVAAN
                     CFLNAFATARRSSRTKWITSINWPRWAGLGMAIQAPSSSVSARRLHPVLGKRVLDTPD
                     AFAYEAKLSFAGHWLLNEHRLRGGDALFPGTGYIELARAALLARFGSGAIQLRNLSFN
                     TPLTVAEGQTTSARISLHKDGDEFTFSVVSGLPSSTACATAAIRYLGDVIPPVLDLPA
                     LRQACNIRQLTFGPEAQNSKQARFIDFGPRWRCLKRIYLGDTEALSELELPEEFASDL
                     QSFTIHPALLDAATGSAMFVIPDYELTQDLYVPVSYKRITLFAALPRKCYCHLRPAPS
                     NTVRKELVAFDLTLMDDEGRVIAEAEEFVLMRMSSTATLSAGRLRPPEHNPVSGDGTV
                     SANAEMSPQDGVEAFRSILASNSPYQIVVAPAGLNVGQQFFTHARPPGGTATRTPSVP
                     IFRDAPRNDVERVLTVWWEELLGARQIGIHDDFFALGGHSLLAVRLLARIEKEFQKSI
                     PLHVLFQESTIERMAARIHEGKHDPAEAIVPLSANGAGPALYCVHSLGGEVASFRHFA
                     SALGSACHLYGIQIPPGQKKPEFASSIETLASYYVSELLTFQPEGPYLLGGWSVGSTI
                     ALEMARQLQIAGREVELLVALDGAPFNT"
     gene            complement(5782..>6402)
                     /locus_tag="VN788_14325"
     CDS             complement(5782..>6402)
                     /locus_tag="VN788_14325"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HXU21756.1"
                     /translation="IEPEEIEAALNQHPAVQAAAVVAREDIPGDKRLAAYVLLLDSNA
                     VGMNELRVYLQQRLPAHMIPSSFAAVDLMPSTPNGKIDKKALLANRPRQGASRAAVKT
                     RGEFERLISSIWREALKTKTVDIHDSFFDLGATSLIVAEVAATLSEELQIDLPLTDLF
                     QYPTIDALAGYLSRNGDHDEVLAANAGFTAGQSRKAAMLVRHRARA"
BASE COUNT         1456 a         1791 c         1792 g         1363 t
ORIGIN      
        1 tcgtattgaa tggcgctcca tcgagagcca caagcaattc aacctcgcga cccgctatct
       61 gtagctggcg ggccatttca agagctattg tcgatccgac tgaccaacca ccgagtaaat
      121 aggggccttc cggctggaac gtaagcagtt cacttacata ataggaagca agtgtctcaa
      181 ttgatgacgc aaattctggc tttttttgtc ccggaggaat ctgaatacca tataagtggc
      241 aggccgaacc caaggccgaa gcgaagtgtc gaaagctggc gacctcgccg cccagagaat
      301 ggacacaata tagagccggc ccggctccgt tggcgctcaa tggcacaatc gcctctgcgg
      361 gatcgtgctt gccttcgtgg atgcgcgccg ccattctctc gatcgtactt tcctgaaata
      421 agacgtgcag cgggatcgat ttctgaaact ccttttcgat tcgggccaac agacgtacgg
      481 ccaggagcga atggcccccc aacgcaaaaa agtcgtcgtg aatacctatt tgccgcgccc
      541 cgagcaactc ctcccaccat actgtcaaca ctcgctcgac atcgttgcgc ggcgcgtctc
      601 ggaagatcgg aaccgatggc gttcgagtag cggttccgcc tgggggcctg gcatgtgtga
      661 agaactgctg gccgacgttg agcccggccg gcgccaccac gatctgatat ggtgagttgc
      721 tcgcgagaat agaccggaac gcttcaacgc cgtcctgcgg cgacatctcc gcatttgccg
      781 aaaccgtgcc atccccggaa acagggttgt gttcaggcgg tcgcagccgg ccggccgata
      841 gtgtggccgt gctactcatc cgcatgagca caaactcttc agcttctgca atcacgcgac
      901 cctcatcgtc catcagagtt agatcaaacg ccactagttc cttgcgtaca gtattcgatg
      961 gggccggcct tagatggcaa taacatttac gcggaagagc ggcgaacagg gtaatgcgtt
     1021 tataggaaac tggcacgtaa agatcctgcg tcagctcgta atcgggaatt acgaacatcg
     1081 ccgacccagt tgccgcatcg aggagcgctg gatggattgt gaacgattgt agatcggaag
     1141 cgaattcttc cggaagctct aactcactca gcgcttcggt atcgcccaga tagatgcgct
     1201 tcagacatct ccaccggggg ccaaaatcaa tgaaccgggc ttgtttggag ttttgcgctt
     1261 cggggccaaa agtaagttgg cgaatgttac atgcctgtcg aagcgcgggc agatccagta
     1321 ctggaggaat tacatcgcct aaataccgaa ttgcggcggt cgcgcaagca gttgatgacg
     1381 gaagtccaga caccacggaa aatgtaaatt catcgccatc cttatgtaag ctgatacgtg
     1441 ccgacgtcgt ctgcccttcc gccacggtaa gtggtgtatt gaacgacaaa ttccgcagtt
     1501 gaatagcccc cgaaccaaag cgggctaaca gcgcggcgcg cgcgagttcg atataacccg
     1561 ttccgggaaa cagcgcatcg ccacctcgca ggcgatgctc attcaacaac caatggccgg
     1621 cgaagctcag tttggcttca taggcaaaag cgtccggggt atcgagtacg cgcttaccca
     1681 gaacggggtg taaacggcgt gcacttacac ttgaggatgg tgcttggatg gccattccca
     1741 ggccggccca tcgtggccag ttgatcgacg taatccactt cgtccggctc gatctccggg
     1801 cggttgcaaa cgcgttgagg aaacaattag cagcaacgta atccacttgg ccgacaggcg
     1861 ctgaccaaca actgatggaa gagaacagaa cgaaaaagtc aagttctgca ttccctagcg
     1921 cggcatccag caccagcgtc cctctgactt tgggcgcaag gacccggtcc gtatcctccc
     1981 ggctcttgaa ttgaattagc ccgtcatcca gaactcccgc ggcatgcaca acaccatgaa
     2041 tcgatccaaa gcgggtctga acttccgaga cgacccgatt catgccggcc aaatccgtca
     2101 cgtcgcctgg aacaatcaag acctctgcgc caagcttctc aatcgcttgt actgcagcga
     2161 tcctatgagt tactgggtca tatccagagt gtctcccctg ataagtttcc caatcgactc
     2221 tcgggggtag ttcagttcgc ccgacgagaa cgagtttcgc ctgtacggtt cgcgccaggt
     2281 attctgcaat gacaagtccg acgccaccca aaccaccagt gatgagatat actccgcgct
     2341 tacgaagacg attgccgtca aggtgcggat cgatttgagt aagttcgagc gtttgtgtcc
     2401 agcggatatc tcgacgatac gcaaccactt gatcgagaga ctccgaagat aactcggcga
     2461 tgagttgagc agcgattccc tgatggccgg catcggatac aggtaactga atgtcgatac
     2521 accggctata gacctttgaa aactccttcg aaatgacttt gccgggaccg agcaggagag
     2581 cgctttccgg gtgctggatt atctctccgt cgatcgagtg gaggccgttt gaaataatcc
     2641 ccagttcaat agaatcaatt tcatcatgtg ctccgagcgc ctgagcaaga aagagcggac
     2701 tgtggaagct caactcctga tgccgctcga gacgatcgag tatcgggtca tttgagtctg
     2761 caagcggcca aagatggagg attcgccgtg gaatttgtcc agagtggatg agatccgtga
     2821 cgagagcttc gtaatcgggg cgatgcccgg ggttgatcga ataactacta tggccggttt
     2881 ttgcgaagtg tttgccgggg ttgacggtga tggccatttc gcctagagcg atgattctcc
     2941 ggacgatttc cgcaccgaca ccgaacgagt cggcgaaaat cagccaattc gagttgccac
     3001 ccgccggtac tgccgccgag gaaagatcta ctttcttcca ggcagggcgg tagtgacaaa
     3061 gcgtagtctg cgcgccggcg agagggacct cgctgagcgc gctgcgattt cgcttcggcg
     3121 catcgatcca gaagcgctgg cgctcgaacg ggtaagttgg caaagggacg cggtagcgcc
     3181 gctcatttgc atgaaaccca gtccatttaa tgttcacccc cgaagtccat agtcgcgcca
     3241 cagtagttaa tagaatcccg acatcgtccg tgttctcgcg tgagtcgcga atagacgaga
     3301 agatcccgtt gtcatgactc gctgtcgcat ggcatggcgc cagtcccgaa agtactttcc
     3361 ccgggccggt ttcaaggaga atagtcgacg gttgaacgag taactcttgc agcccttcgg
     3421 aaaagcggac cgtttgtcgc acttggcggg cccagtaaat gggatcagta acctcttcat
     3481 gcttgatcca agtaccggtg atgttcgaga ggtacggaat tgccggccga ttcagttgaa
     3541 ctccacgcat cacgtccgca aaccgttcga gaatggggtc cattgccgcc gaatgaaatg
     3601 cgtgggaagt atgtagccgc cggcaggaga ttccgtcgcg gcccagcgcg tcctctagcc
     3661 ctccaattgc ctccgtggaa cccgaaaaga cgcactgctg ggggccgttt attgcgccga
     3721 tggaaagatc atgagacacg aagcgatgcg catcgcgctc cggcattgca gcggcgagca
     3781 tcgctcctcc ctccatcgac tgcatcaact ggcctcggcg agcgaccacg aacagggcat
     3841 cctcgagaga cagtacgccc gctacgcacg ccgcgacaaa ctcaccaatg ctgtggccga
     3901 tcatcgcatc gggacaaata ccccaagaca tccataactt agccagtgca aactcgataa
     3961 cgaataacgc gggttgagta agccacgttt gcgctaacct atcgctagcc tgggcaaggc
     4021 cggcggctgg cggataaagg accgttcgaa gatcgaggcg aagttgcggt ttaagtaact
     4081 cggcgcagtg gtcgacttga gagcggaact ccttctccgt ttcataaatg ccgcgggcca
     4141 tgttcacgta ttgcgatccc tggcccggga aaagaaaggc cacacccgtg tgattcacgg
     4201 cgtgtccagt gaaaacgcgt tttggatttc cggagcgcag tgagacaact gcatcttcgc
     4261 gcgtttgaca cagcaccgaa cgtcgatgcg aaaaagcttt gcggccaagg tggcaggtgt
     4321 aggagacatc cgcgagattc agaccgtcac tggcttccag gtgctcagcg aggtttgccg
     4381 cggcggactc aagcgcagtc gcggttttcg ctgaaatcgt gaggatctgc catggcctgg
     4441 aatcgcccgg cggctcggtt gggggtgctt cctcgaggat gatgtgcgcg ttggttcctc
     4501 caattcccag ggaagttact ccggcgcggc gtggtgcgcc gtttgtctcc cacgtagaga
     4561 gagttctatt gacgcggaac ggactattct cgaaatcaat ggcgggattt gactttgaaa
     4621 aatgtaagct ggggggaatt tgcttgtgtc tgagagccag aattgttttg atggcgccgg
     4681 cgatgccggc ggcggcgtct agatggccaa tattcgtttt tagcgaccct atacggcaga
     4741 aaccgtgcct ttgcgtgctt ctccggaatg cctgggtaag tgccgcgatc tcgattggat
     4801 ctccaacttg tgttccagtg ccgtgcgtct caatgtaaga gatagactcc gccgtaactc
     4861 cagcgactgt aagcgcctcg aggacgacat cggattggcc ggagacgctt ggcgcgagat
     4921 agcccacctt tcgagctccg tcgttattga tcgccgttcc cctgataaca gccaggatgt
     4981 tatccccgct ggcgagagca tcccgtaaac ggcgcaatac aacgatgccc gcaccgcttc
     5041 cgaagacggt tccggtagat tgcgcatcaa acgcgcggca gtggccgtcg cgagaaagaa
     5101 tttcgccctc gcgatagaga tagccgttcc cgtgtggaat ctcgatcgtg actccaccgg
     5161 ccagcgccat gtcacactca tgcgctaaca gactctggca ggccatatgc acggccacga
     5221 gcgacgttga acaagccgtt tgcactgtca tgctcggtcc cgtgagattc aactgataag
     5281 aaacccgcgt cgctaacacg tccttatcgt tccctgtctg gcgaatcaga aacaggccgg
     5341 cgctgtccat taattgacgg ttcggcagta gattgaaaat caaatacgaa ttcatgccgg
     5401 aaccggcgta cactccgatc ggcccggcaa actcgtccgc gctccagccc gcgtgttcga
     5461 gagcttccca tgcgcattcg agaaaaacgc ggtgttgtgg gtccatgatc gccgcgtcct
     5521 tcggactgaa gccgaaaaag gaggcgtcga acatgtcgat atcgtcgagt acggcggcgg
     5581 cttttacgta gtgcggatcg tgtaactcaa tcggatcgac gcccgccgcg cgaagttgat
     5641 cgtggctgag cggaacgatg gattcgatcc cgttccgaag gttgttccaa aactcggaaa
     5701 gacttcgcgc accgggaaag cgtccagcca tgccgataac ggcaatcgcg gaagaatcgg
     5761 gatccgccag tggcgcgtcc gtcatgctcg agccctatgg cgtaccaaca tcgcagcctt
     5821 ccgggattga ccggctgtaa aacccgcatt cgcggccagg acctcatcgt gatcgccgtt
     5881 ccggctcaga tatcctgcga gtgcgtcgat cgttggatat tgaaaaagat ccgtaagtgg
     5941 aaggtctatc tgcagctctt cactgagcgt cgccgcaacc tcggccacta tcagtgaggt
     6001 tgcccctaaa tcgaagaagc tatcgtggat atcgactgtt ttcgttttga gcgcttcccg
     6061 ccatatgctg gagataagcc gctcgaactc gccacgtgtt ttcacggcag cgcgggaagc
     6121 tccttgccgg gggcgatttg ccagaagcgc ctttttatcg atctttccat ttggagtcga
     6181 gggcataagg tccacggcgg caaacgaaga aggaatcata tgggctggta gccgttgttg
     6241 caagtagacg cgcaattcat tcatcccaac cgcattacta tccagcagaa gcacgtacgc
     6301 cgcaagtctt ttgtcgcccg gtatatcctc ccgagcaaca accgcggcag cttgcacggc
     6361 cggatgctga ttcaatgcgg cttcgatctc ttccggttcg at
//
LOCUS       DATNDT010000438         8016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_687522, whole genome shotgun sequence.
ACCESSION   DATNDT010000438 DATNDT010000000
VERSION     DATNDT010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8016
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687522"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..247
                     /locus_tag="VN788_14330"
     CDS             <1..247
                     /locus_tag="VN788_14330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HXU21757.1"
                     /translation="CRDSILAAPIVLDLALFFDLAKRAGMRGIQEWLSFYFKSPMCAR
                     TVYPEHDLFIQLMKLKNTLRWMMGEDLITHLGAEYYD"
     gene            complement(563..1075)
                     /gene="pdxT"
                     /locus_tag="VN788_14335"
     CDS             complement(563..1075)
                     /gene="pdxT"
                     /locus_tag="VN788_14335"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HXU21758.1"
                     /translation="MLERLGVEHRFVRRPDELLGLAGVILPGGESSTHLKVMNEEGLF
                     DALKQFAADGGAFFGTCAGAILLAREVHGPAQKSLGLLDISILRNGYGRQLASDVHLG
                     PTKLRKEPLEMVFIRAPIIESTGEGIEVLAEDAGHPVLVQQGRVLAATFHPELTTETV
                     VHEHFISSLK"
     gene            974..1276
                     /locus_tag="VN788_14340"
     CDS             974..1276
                     /locus_tag="VN788_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21759.1"
                     /translation="MRAALPTRKDHASQAKQLVRPPHKAMLDAKPLKHLRVSLIVPLN
                     RNNPNLHPVHQLAITTRSRRGVALLRPLNMFGYLLTIRQAVRVILSAILPSTDVLL"
     gene            complement(1785..2678)
                     /gene="pdxS"
                     /locus_tag="VN788_14345"
     CDS             complement(1785..2678)
                     /gene="pdxS"
                     /locus_tag="VN788_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HXU21760.1"
                     /translation="MEAMNGNSLWRVKVGLAEMLKGGVIMDVTNAEQAKIAEDAGACS
                     VMALERVPSDIRKEGGVARMASPRVIREIMDTVSIPVMAKVRIGHFAEAQVLEELEVD
                     YIDESEVLTPADEEHHVWKHGFKVPFVCGARNLGEALRRIAEGAAMIRTKGEAGSGNI
                     VEAVRHMRAIVGQTKRLAALGVEELVHEAKEIGAPLELIQWVAKNGKLPVPNFSAGGI
                     ATPADAALVMQLGAEAVFVGSGIFKSADPAQRARAIVKATTHYKDPKVVLEASEELGE
                     AMKGLDVRQMDEKDLLQTRGW"
     gene            complement(2734..3216)
                     /locus_tag="VN788_14350"
     CDS             complement(2734..3216)
                     /locus_tag="VN788_14350"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HXU21761.1"
                     /translation="MGERMLLKPNATNHCFGCGGANARGMQLTFEQDDEARQIRGIMR
                     LGAEYQGGAGFVHGGIVATLLDEVMAKVNRFGQDYAVTAQLTVEYLKPVPVDEELIVN
                     GWEVERDGRNLLREGEIRDASGVLLARGKGRFVSIDPKKFRPGIATADASEQADGKRI
                     "
     gene            complement(3223..4761)
                     /locus_tag="VN788_14355"
     CDS             complement(3223..4761)
                     /locus_tag="VN788_14355"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HXU21762.1"
                     /translation="MLPLHLQPESHIPLYMQLRDQLRGLVHSGELQTGDRIPASRELA
                     VQLGVHRTTVANAYAELESEGLIQGHVGRGTFICSLPIKQFSPAPRANGNGGPARWDA
                     LFADERGDEGLSRLMPEVPAGAIAFTCARPSEESFPLEEFRRCTNAVLKREGRRILQL
                     GATDGYEPLRCALIEWFRSEGLNIKDEQLLITDGGQQAIDLVCKAFIRAGDSVALENP
                     AYPGAISILMGARARMLPVPVETDAERSGFAGLDVDALENILLQNRVKFIFVTPDFHN
                     PTGTAMPAPRRRRLIEIAARYQVPIVEDSIYARTRVRGSALPSLRSLDTNGNVIQIDS
                     FSKIAFPGLRVGWCIGAPSAIERLRLVKQSTDLHTDQLSQAALAEFIRRGHLTRHLAK
                     MKKVHLSRLEAMEAALEKHMPERTSWTRPEGGMTVWVTLPAGFDAGDLLIHARERGIY
                     FVPGRHFYSQSPRANTLRLGFSSVDEKSITRGIEIFCDVVKEELRKRQRGVRDDVRAR
                     VALV"
     gene            complement(4915..5421)
                     /locus_tag="VN788_14360"
     CDS             complement(4915..5421)
                     /locus_tag="VN788_14360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21763.1"
                     /translation="MKKRDPASALKKVVVLLLDRSILKGYLDARTLGRSEAMDLLTPD
                     GEHHSMQLREIKSVYFVREFADSFEPERKTFLSRPKIDGLWVRLRFQDEDVMEGIVAN
                     DLIELLDGGVRLTPPDLHGNVLHMFVPRTALAEVKVLGVVGAARRTPRATREIAAVLE
                     AQSKLFGE"
     gene            5556..7151
                     /gene="murJ"
                     /locus_tag="VN788_14365"
     CDS             5556..7151
                     /gene="murJ"
                     /locus_tag="VN788_14365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HXU21764.1"
                     /translation="MLRSASVVAFVTIISRICGYLRDQRVTLLLGTSPAADAFVLAFR
                     IPGLVRRMMGEGSLGASFIPVFTKYLRTNTPRETWTFAERMFWDLALFLAVIAGLGAI
                     FSRQVIGIYTFLGANGSAWSLATHLNRIIFPAIFFIGLGAVAAAILNSLGAFAVPAAS
                     SVFFNLGLIACSIGVVYRPLLRWIPGGFRGPAVALAAGVLLGGILQLSVQVPSLIRRG
                     MSFAPKFSFSDPGVRKVARLMGPSFFGMGVYQINIFVDTIFATSSRMPAGSVTSLYLG
                     DRVMQLVLGSYAIAMSTALLPAMSHQQAAGEGEQVKDTFGFSLRIVSFITIPAAVGLF
                     LLRRPIIQVLFQHGQFSPDSTSLTAHALLFYSLGLPAFAAIRIITPMYYSAQDTATPA
                     RVGAYALGLNILLNSVFLLFLFRALSNGSPALASSLTAYFNFAALYFLFRKRHGGLYG
                     RVLLQSLGRISVCAAAMGIACYAILGASHFSEAGHLTAQAGLLAAVIAASTAAYLVLA
                     WVFRCDELGELLLVLRRGKARTV"
     gene            7267..>8016
                     /locus_tag="VN788_14370"
     CDS             7267..>8016
                     /locus_tag="VN788_14370"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HXU21765.1"
                     /translation="MEANVRAFVNSLRVEKGLSENTIHAYRRDMLKFMAFVTERGLKT
                     ADIERSHVVEFLASLYHKGLDSRSVARHLVTIRHFFRFALMEEWIAEDPAATVQSPKF
                     RQSLPEFLSLTEVDRLLEQPDTGTVVGLRDRAMIELMYSCGLRVSELCGLRIGDLRME
                     DGCLRCIGKGDKERLVPVGKRALAIVAKYLRDSRPKLVREGSTPHLFLNQKGAMVNRI
                     AYWKMLGIYGRKAALRKAVTPHMLRHSFATHL"
BASE COUNT         1579 a         2558 c         2073 g         1806 t
ORIGIN      
        1 ctgtcgcgat tcgattctcg ccgcgccaat cgtcctcgat ctcgctttat ttttcgactt
       61 agccaaacgc gccggcatgc gcggcattca agaatggctc agcttctatt ttaagtcccc
      121 catgtgcgcg cgcaccgtct atcccgagca cgacctcttc atccagctaa tgaaattgaa
      181 gaacacactc cgctggatga tgggcgaaga cctaatcacc cacctcggcg ccgaatacta
      241 cgattagtct tctcttcgtc ggggcatagc atgctatgcc cggcgccaat acttggctac
      301 tagtcaatta tccgccgagc cgagcgtgat cttgccgtca gccgagcgcc ttcgccatcc
      361 gcggccgtca ttccgagcga agtgtcccgc gcctttgctc ttgcgcgaag cgccgggaca
      421 cgcagtcgag gaatctctct tggaacgagc agagaggaag ccttcgcgtg ggggcacgat
      481 acatcgtgcc cggcaaacat acttggcttg ccatccactt cgcgtcggcg cctcatgcac
      541 acgcgagagc gccttcccag aattatttca aactactaat aaaatgttca tgcaccaccg
      601 tctcggtcgt caactcagga tgaaaagtcg cggccagcac acgcccctgc tgcacaagca
      661 ccggatgccc ggcatcctcg gcaagcacct caattccctc tcccgttgac tcaataatgg
      721 gcgcgcggat aaaaaccatc tccagcggtt ccttgcgcag cttcgtcggc cccaaatgaa
      781 catcgctcgc aagctgccgc ccgtaaccat tcctcaaaat cgaaatgtcg agcagcccga
      841 gcgacttttg cgccggcccg tgcacctcgc gcgccagcaa aattgcgccc gcgcaagtac
      901 cgaagaacgc cccgccatcc gcggcaaact gcttcagcgc atcaaacagc ccctcctcgt
      961 tcatcacctt caaatgcgtg ctgctctccc cacccggaag gatcacgcca gccaagccaa
     1021 gcagctcgtc cggccgcctc acaaagcgat gctcgacgcc aagccgctca agcatcttcg
     1081 cgtgagcctc atagtccccc tgaatcgcaa caatcccaac cttcatcccg tccaccaatt
     1141 ggcaatcacc acccgcagtc gtaggggcgt agcactgcta cgcccgctaa acatgtttgg
     1201 ctacttgtta accatccgcc aagccgtccg cgtcattctg agcgcgatct tgccgtcaac
     1261 cgatgtgctc ttgtagcgcc ggcttccagc cggcgttttg tctcgtccag ccatcaacgc
     1321 cgtcattccg cctcccgacg aaggaaggga acaaagtgcg ccgatttttt gtttccgttc
     1381 gcgccttcca gcgaacgtcg gcgcacgcag tcgaagaacc tctcttggaa ctagcacaaa
     1441 ggaagtcttc atcacccgct tcgtagggca cgatacatcg tatccggcaa acatacttgg
     1501 ctacctgatt gtcatccgcc tcgcatcacc ggcgccttat gtggagtgcg ggagcttgct
     1561 cccgccgttt gccgtccggg ctagcccgga catgctgcaa ctatacacac ccgagagcac
     1621 catcatcctc gcaacccatc tccgtcattc cgagcggagt gtcccgcgcc tttgcttttg
     1681 cgcgaagcgc cgggacaggt agtcgaggaa tctctcttgg aattaccaga gaggaaatct
     1741 caggtttctc taacccctaa cctcaaacct ctaacctcta aatctcacca tccccgcgtc
     1801 tgcaacaaat ccttctcgtc catctgccgc acgtccaggc ccttcatcgc ttcgccaagc
     1861 tcctcggaag cctccaacac tactttcgga tccttgtaat gcgtagtggc tttgacgatc
     1921 gcccgtgctc gctgtgccgg gtcggccgat ttgaaaatcc cggagcctac gaataccgct
     1981 tccgctccaa gctgcatcac cagcgcggcg tccgctggag tggcaatgcc gccggccgaa
     2041 aaattcggca ctggcagctt tccgttcttc gccacccact gaatcagttc aaggggtgcg
     2101 ccaatttctt tcgcctcgtg cactagttct tcaacgccga gcgccgcaag acgcttcgtc
     2161 tgtcccacaa tcgcacgcat gtgccgcacc gcttcgacga tatttcctga gccggcctcg
     2221 cccttcgtgc ggatcatggc tgcgccctcg gcaattcgcc gcaaagcctc cccaagattc
     2281 cgcgcgccgc aaacgaacgg caccttgaat ccatgcttcc acacgtgatg ttcttcgtcc
     2341 gccggcgtca gcacttcgct ctcgtcgatg taatcgactt ccagctcttc cagaacctgc
     2401 gcctccgcaa agtggccgat ccgcaccttg gccatcaccg gaatcgaaac cgtgtccatg
     2461 atttcgcgaa tcacacgcgg cgacgccata cgtgccacgc cgccttcctt gcgaatgtcg
     2521 gacggcacgc gttccagcgc catcaccgag cacgcgcccg cgtcctcggc aatcttcgct
     2581 tgctcggcgt tcgtgacgtc catgatcacg ccgcccttca acatctctgc cagtcccact
     2641 ttcacgcgcc acaagctatt tccgttcatc gcttccatcg cagttcacct tttcttccgg
     2701 ccgcttcgaa tcgtgccgga gacattcgcg aatctaaatt cgtttcccgt ccgcctgctc
     2761 gcttgcatcc gccgtcgcaa tccctggccg aaatttcttg ggatcaattg agacgaaccg
     2821 ccctttgccg cgtgccagca atacgcctga ggcatcgcgt atctcgccct cccgaagaag
     2881 attgcgccca tcgcgttcca cttcccatcc attcacaatc aattcttcgt ccaccggcac
     2941 cggtttcaga tattccaccg tgagttgcgc ggttaccgcg taatcctggc cgaagcgatt
     3001 taccttggcc atcacttcgt ccagcagcgt agcaacaatc ccgccgtgga cgaatcccgc
     3061 tccaccctga tattccgctc ccaagcgcat gatcccccga atttgacgcg cctcatcatc
     3121 ctgctcaaag gtcaactgca ttcctcgcgc atttgcgcct ccgcagccaa aacagtgatt
     3181 cgtcgcattc ggtttcagca gcatccgctc acccattccc agttagacca gcgccacgcg
     3241 agcgcgcaca tcgtcccgaa cgccgcgttg ccgcttgcga agctcttctt tcaccacgtc
     3301 acaaaaaatt tcaatccctc gagtaatact tttttcatcc acgctggaaa atcccagccg
     3361 cagcgtgttc gctcgcggac tttgcgaata gaaatgccgc cccggcacaa aataaatccc
     3421 gcgttcccgc gcatgaatca gcaaatcgcc ggcatcgaat cccgccggca gcgtcaccca
     3481 caccgtcatc ccgccttccg gacgtgtcca gctagtcctc tccggcatgt gcttttcaag
     3541 cgcggcttcc atcgcctcca atcggctgag atgcactttt ttcattttcg ccaaatgccg
     3601 tgtcaggtgc ccgcggcgga tgaattccgc cagtgccgct tgagaaagct ggtccgtgtg
     3661 caggtccgtg gattgcttca ccaggcgcag acgctcaatc gcgcttggcg ctccgatgca
     3721 ccagccaacg cgcaagccgg gaaacgcgat ctttgaaaag ctgtcaatct gtatcacgtt
     3781 gccattcgta tcgagcgatc gaagggatgg caacgcgctc ccacgcaccc gcgtgcgcgc
     3841 gtaaatgctg tcttcaacga tcggcacctg atagcgtgcc gctatctcga tcaaccgtcg
     3901 tcgccgaggc gccggcatcg ccgtgcccgt gggattgtga aaatccggcg tgacgaaaat
     3961 gaacttcaca cgattctgca gcagaatgtt ttcgagcgcg tccacatcca accccgcaaa
     4021 gccagagcgt tcggcatcgg tctcgaccgg caccggcagc atccgagcac gcgcgcccat
     4081 caaaatcgaa atcgctcccg ggtaagcggg attttccagc gcaaccgaat ctcccgcgcg
     4141 gatgaacgcc ttgcaaacca gatcgatcgc ctgctgccca ccgtccgtta tcagaagttg
     4201 ctcatccttt atgttgagcc cttcgctgcg aaaccattca atcagcgcgc agcgcaatgg
     4261 ctcgtatccg tccgtcgctc cgagttgcag aattcgccgt ccctcgcgct tgaggaccgc
     4321 gttcgtgcag cgccgaaact cttccagcgg aaaagattcc tccgaaggac gcgcgcacgt
     4381 aaaagcgatc gctcccgcgg gcacctccgg catcaggcgg ctcagtcctt cgtcgccgcg
     4441 ctcgtccgcg aaaagcgcat cccaccgcgc aggacctccg tttccgttcg cgcgcggtgc
     4501 gggcgagaat tgtttgatcg gcaagctgca aataaatgtc ccccggccaa cgtgcccttg
     4561 aatcagtccc tctgattcga gctccgcgta ggcgttcgcc acagtcgtgc gatgcacgcc
     4621 gagctgcacg gccagttccc tgctcgctgg aatgcggtcg ccggtttgaa gttcgccgga
     4681 atggaccagc ccgcgaagct gatcgcgcaa ttgcatatac aatggaatgt ggctttctgg
     4741 ctgtaaatga agcggtaaca ttggctcctt ccttcaggcc aatatagagt acaccagcaa
     4801 cctgtaaaga gccaatttgg accagtgctg ctaaaaaatc cttctagttt gccgcaagaa
     4861 atgcttctag cttgaattct aactagaaac aatttaaggc aagtttgggc gcggttactc
     4921 gccgaaaagc ttggattgcg cttccaggac agcggctatt tcgcgggtag cccgcggcgt
     4981 ccgccgcgcc gccccgacga cccccagtac cttcacctcg gccagcgcgg tccgcgggac
     5041 gaacatgtgc agaacatttc cgtggaggtc agggggcgtc agccggactc ccccatccag
     5101 cagctcgatc aaatcgttgg caacgatgcc ttccatgacg tcttcgtcct gaaatcgcag
     5161 gcgaacccat aaaccgtcta ttttcgggcg gctaaggaaa gtcttacgct ccggctcaaa
     5221 ggaatcggca aactctcgca caaaatacac tgactttatc tctcggagtt gcatggaatg
     5281 gtgttctccg tcgggtgtaa gcaggtccat ggcctcggaa cggccaagcg tccttgcgtc
     5341 gagatagccc ttaagaatgg atcgatctag aagaagcacg accactttct tgagagctga
     5401 cgctggatcg cgttttttca cgccgccaaa ccttttccgg cagggaggtt gttgctatgt
     5461 tctcgcggac tatgttatct ttgtctcggt atttgtcaac tcattggtgc ttaaagggtt
     5521 agcgtcggag ccggctgatc gaaagcaaag gacaaatcct acgatcggcc tccgtcgtag
     5581 ccttcgttac cattatcagc cgcatttgcg gatacttacg cgatcaacgg gtaactctcc
     5641 tcctcggcac atcgcccgcg gccgacgcct tcgtcctcgc cttccgcata cctggacttg
     5701 tacgccgtat gatgggggag ggttcgctcg gcgcttcttt catcccggta ttcacgaaat
     5761 atcttcggac aaacaccccg cgcgaaacgt ggacctttgc ggagaggatg ttttgggacc
     5821 tggcgctttt cctggcggtg atcgcgggtc ttggggcgat tttttcgcgg caagttattg
     5881 gcatatacac atttctggga gcaaacggct cggcgtggag tctggccacc catctgaatc
     5941 ggatcatttt tccggcaata tttttcatcg ggcttggcgc ggtggcagcc gcaatcctca
     6001 acagcttggg cgcgtttgcg gtgccggcgg catcgtccgt gttcttcaac cttggtctga
     6061 tcgcttgctc catcggagta gtctatcggc cgctgctgcg atggatcccg gggggattcc
     6121 gcggtccggc cgtcgcactt gccgcaggcg ttttactggg cggcatttta cagctttccg
     6181 tgcaggtgcc gtcgctgatt cggcgcggaa tgagctttgc gccaaagttt tctttttccg
     6241 atcccggcgt gcgcaaagtg gcgcggctga tggggccgag ctttttcggc atgggcgtgt
     6301 atcagatcaa cattttcgtg gacacaattt ttgccacgtc ttcgcgcatg cccgcgggca
     6361 gcgtcacctc tctttacctg ggagatcggg tgatgcaact ggttttggga agctacgcga
     6421 tcgccatgtc cacggctctt ttgccggcga tgtctcatca gcaggccgcg ggggaaggcg
     6481 agcaagtgaa ggacactttt ggcttctctt tgcgaatcgt atctttcatc actattccgg
     6541 cggctgtggg attatttctg cttcgccgcc cgatcattca agtgcttttc cagcacgggc
     6601 aattcagtcc ggactcgacc tcgcttacgg ctcacgccct tctcttttat tcgctgggct
     6661 tgccggcgtt cgcggccatc aggattatta ctccgatgta ctactcggct caggatacgg
     6721 ccacacccgc gcgtgtgggc gcttatgcgc tgggcctgaa tatccttctc aattctgtat
     6781 ttttgctgtt tctatttcgc gcgctttcca acggaagtcc ggcgctggcg agttcactta
     6841 cggcgtactt caatttcgcg gctctctatt ttctatttcg caaacgccac ggaggattgt
     6901 acgggcgcgt tctacttcaa tctctaggca ggatttccgt gtgcgccgcg gcgatgggaa
     6961 tcgcttgcta cgcaatattg ggcgcgtcgc atttttcaga agcgggccat ctgacagcgc
     7021 aggcggggct gcttgccgca gtgattgcgg cttcaacggc tgcctatttg gtattggcat
     7081 gggtttttcg ttgcgacgag cttggggagc tcctgttggt gcttcggcgc ggaaaagccc
     7141 ggaccgtcta gctcgggccg cgacaacgat tcggttagga gactggacaa tcacccgtga
     7201 ggaccgggtg agctacatcg aaaaccatag gagcgcgtag cagccgcgag agtcgaggaa
     7261 aaaaacttgg aagcgaacgt tcgagcattc gtaaacagcc tgcgggttga aaaaggcctt
     7321 tcggaaaaca ccattcatgc ctatcgccgc gatatgctga agttcatggc attcgtgacc
     7381 gagcgcggcc tcaagacagc cgacatcgag cgcagccacg tggtggaatt tctggcgtcg
     7441 ctgtaccaca agggcctaga tagccgctcg gtagcgcgcc atctcgtgac catccggcac
     7501 tttttccgtt ttgcgcttat ggaagaatgg attgcggaag atccggcagc gaccgtccaa
     7561 tcccccaagt ttaggcaaag ccttcctgaa tttctcagcc tgacggaagt ggatcgcctg
     7621 ctggagcagc ctgacacggg cacggtggtg ggcctgcgcg atcgggccat gatcgagctg
     7681 atgtattcct gcgggctgcg agtctccgag ttgtgcgggc tgcgcattgg ggatttacga
     7741 atggaagacg gctgcctgcg atgcatcggc aaaggcgaca aggagcggct ggttcccgtc
     7801 ggcaagcgag cgctcgccat tgtggccaaa tatctgcgcg attcgcgccc caagctcgtg
     7861 cgcgagggtt ccacgcctca cctttttctt aaccagaagg gcgcgatggt gaaccggatc
     7921 gcttactgga aaatgctggg aatctacggg cggaaggccg cgcttcggaa ggcagtgacg
     7981 ccgcatatgc ttcggcacag cttcgcaact cattta
//
LOCUS       DATNDT010000439         4969 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3630364, whole genome shotgun sequence.
ACCESSION   DATNDT010000439 DATNDT010000000
VERSION     DATNDT010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4969
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3630364"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1054
                     /locus_tag="VN788_14375"
     CDS             <1..1054
                     /locus_tag="VN788_14375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21766.1"
                     /translation="MKLRISLFLALLAAGVLVVGLHAVHAQNAQGAHYTTRTLALPDH
                     GKGNITMDYIAYDPKTGYVWVPAINIGSVDVVDTSNNSVREIPGFATNAVERGARMRV
                     QGPSGVSIGDGVVYIGDRADMSICAVDEKTLARKSCGHIDSTPDGVVYVAPTKEVWVT
                     APRDNSVRILDSSTLAQKEKLTFDGRPEGYAVDARRGRLYMNYEDKDLTTAIDLKTHK
                     TVAKWPSSCGEDGPHGISVDQQSGFLFVACSTRAEVLDAGHNGEKLSSIDTGDGVDDL
                     NYSPATHTLYVGAAEAARLTVAHVDDAGKLTLVAQVPTHEGARNGVVTKNGTVYLAHA
                     RPGEFAGLIVASPAKR"
     gene            1178..1423
                     /locus_tag="VN788_14380"
     CDS             1178..1423
                     /locus_tag="VN788_14380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HXU21767.1"
                     /translation="MKVSVADAKNRLPELVKAVENGEAVTICRRGMPVADLVPTRAPS
                     DEKPRFGTLRGKIIESDPDWWKPMSEEEVDALLDGRS"
     gene            1424..1804
                     /locus_tag="VN788_14385"
     CDS             1424..1804
                     /locus_tag="VN788_14385"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HXU21768.1"
                     /translation="MQVLIDTVVFIWALVSPERVSKRATALLNKSDTVRLLSAVSVSE
                     LAIKQARGKLAINKEQVSAGVSDLRLRILPYTAQHAYELFELPLHHFDPFDRQIIAQA
                     ISEDIPVVTSDEKFSLYKGLKVIW"
     gene            2047..2847
                     /locus_tag="VN788_14390"
     CDS             2047..2847
                     /locus_tag="VN788_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014786733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21769.1"
                     /translation="MNQPVLAANAECPLCGGSGWKSGPAAAGKSKAVVRCDCRLGNWS
                     ERLLKAARIPARYEHCTLSDFESDFPGANRSLASALFQANRFVEEYPVETTGLMLLGP
                     IGVGKTHLAVGILRELVTQKGANCLFYDYRELLKAIQNSYNSTVQATELEILQPVFET
                     EVLVLDELGAVKPTEWVWDTVSHILNTRYNDKRTTIITSNFDDLPPSSRLAGDEAMAA
                     SRRIAARAARDETLGDRIGERMRSRLHEMCRKIEMEGGDFRQSIRQAH"
     gene            3003..4049
                     /locus_tag="VN788_14395"
     CDS             3003..4049
                     /locus_tag="VN788_14395"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HXU21770.1"
                     /translation="MAHVGTLRDFRFADNADDVRGAALYGRDNDKLGKIDDVIFDHET
                     GALRYAVVDTGGWLSSKRFVVPADRIRSRGDKDDEYAVNLTKKQIETFPAYDEKHLED
                     EKRWSDYEKNYRKASGFEETAGILHEAGGTNVLIPDDIPATGPPPTGPGGEPISGYKS
                     PIRHRNTGLMNTTPTGWGEDPDNERMEHVNDSLATDRGDIEDRQIDESAAAKTASPMR
                     EVGEAADISRSTRDRDIANREKDRTYHTDRGSKLSAEEQTMEGDSVFNSADIQDRMGA
                     KRSPRSADVPNYAVIAGGETQSSQGRTPNYPDASQGQRWARFEENLRRQRPNIVGRCG
                     ICEGMQNKHREDVA"
     gene            complement(4216..>4969)
                     /locus_tag="VN788_14400"
     CDS             complement(4216..>4969)
                     /locus_tag="VN788_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21771.1"
                     /translation="AEIERRSELRGELASYLRALEKRMRAPRPEVFLTLHGLPLAMQQ
                     FKWPFHRSTSGADSYILHGIAQLAEPESPLHAKVSASLTVTFAEVLPALEQPYAESVT
                     YNAIRKTLDLGQLELLKSGNRQPVPVSTRYYSFRQKRFIFNDTSDQQRKDFLQSKIFW
                     ISGRLGNRKSVWIADPYDAQYLDCSLDDLRQIARELAKEGLVTLDSDGEYAATTDKLV
                     ARSGEYESKMHSALALLKPKFNEEMRAGHQNM"
BASE COUNT         1115 a         1329 c         1479 g         1046 t
ORIGIN      
        1 catgaaactg cgaatctcac ttttcctcgc gctcttggcc gcgggagttt tggtggttgg
       61 gttgcacgct gtgcacgcgc aaaacgctca aggcgctcac tacacgaccc gcactctggc
      121 gcttcccgat catggcaagg gtaacatcac gatggactac attgcctacg atcccaaaac
      181 gggatacgtg tgggtgcccg ccattaatat cggctcggtc gatgtggtgg acacgagcaa
      241 taacagcgtg cgcgagattc cggggtttgc gaccaatgcg gtggagcggg gcgcacgcat
      301 gcgagtgcag ggtcccagcg gggtgtcgat tggggatggc gtagtttaca tcggcgatcg
      361 cgccgacatg agcatctgcg ccgtcgatga aaagacgctg gcgcgcaaga gctgcggaca
      421 catcgattcc acccccgacg gggttgtgta tgtcgcgccg acgaaggaag tctgggtgac
      481 ggcgccgcgc gacaattcgg tgcgcattct ggacagttcc accttggcac agaaggaaaa
      541 gctgactttt gatgggcgtc cagaaggcta tgcggtcgat gccaggcgcg gacgcctgta
      601 catgaactac gaagacaagg acctcaccac cgcgatcgat ctcaagacgc acaaaactgt
      661 cgccaaatgg ccgtcgtcat gcggagagga cgggccgcat gggatttcgg tggaccagca
      721 gtcgggcttc ctgttcgtcg cctgcagcac ccgggccgag gtgctggatg ccgggcacaa
      781 cggggagaag ctgtcatcga tcgataccgg cgacggcgtg gatgatctta actactcgcc
      841 ggccacgcac accctctatg tgggcgccgc tgaagctgca cgactgaccg ttgcccacgt
      901 tgacgacgca ggcaagctca ccctggtcgc ccaagtgccg acgcacgagg gcgctcgtaa
      961 tggtgtggtc acgaaaaacg gcacggtcta tctggcgcac gcgcggcccg gtgaattcgc
     1021 gggactgatt gtggcatcgc cggcaaaacg gtgatccttg cggagctgag gttattcccg
     1081 aattggaaca gaggatttca ggaacactcg cagccaacgc ctggtccttg tcaactggat
     1141 tgaaatagct atctcaaatg gctataatgt agttctcatg aaagtcagtg ttgcagacgc
     1201 caaaaaccgg cttccggagc tggtgaaggc ggtggagaac ggcgaagcgg tcaccatctg
     1261 ccgcaggggg atgccggtcg ccgacctggt tccgacgcgg gcgccgagcg acgagaaacc
     1321 tcgattcggc accctgcggg gaaaaattat tgagtctgac ccggattggt ggaagccgat
     1381 gtcggaggaa gaagttgacg cgttgctgga cggccggtcc tgaatgcagg ttctcattga
     1441 caccgttgtc ttcatttggg cgttagtctc gccggaacga gtttcaaaac gggcaacagc
     1501 gcttctgaac aaaagcgata ccgttcgatt gctgagcgcc gtttccgtct cggaactcgc
     1561 gatcaagcag gcccgcggca aactggcaat caacaaggag caagtgagcg ccggagtgtc
     1621 tgacctgcgt ttgcgcatcc tgccctacac agctcagcat gcctacgaac tgttcgagct
     1681 gccgcttcat cacttcgacc cattcgatcg ccaaatcatt gctcaagcta tttccgaaga
     1741 tattccagtc gtcacttccg acgagaaatt cagcttgtac aaagggctga aagtgatctg
     1801 gtaagaagac aagctatcga gccggcagaa tacaggagcc aattggaccg aagttcattt
     1861 cgagcagttg tgaactatcg gtccgatccg taacatccgt gtcatccatg tgagcttttg
     1921 ctttttttgc aaaaacttgg cgagcttcac tgcctgtgta ttcatcggct gcaccattct
     1981 ttggcgttgt ctcgtttgcc gctctctcct atcatcgcgt tcgactcgca ttgaaatatt
     2041 cacaacatga atcaacctgt tttggctgca aacgctgagt gtccgctgtg tggcggttct
     2101 ggatggaagt ctgggccggc agcggcggga aaatcgaaag ccgtcgttcg ctgcgactgc
     2161 cggctgggga actggtcgga gcggttgctg aaggcggcgc gcattccggc gcggtatgag
     2221 cactgcactt tgtcggactt tgagtcggac ttccccgggg cgaaccgcag cctggcttcg
     2281 gcgctttttc aggccaaccg ttttgtcgag gaatacccgg ttgagaccac cggattgatg
     2341 cttctgggtc ccatcggggt aggcaaaact cacttggcgg tcgggatttt gcgcgaactg
     2401 gtcacgcaaa agggcgctaa ctgtctcttt tatgactacc gagagctctt aaaagccatt
     2461 cagaactcct acaattccac ggttcaggcc actgaactgg agattctgca gccggttttc
     2521 gaaacggaag tgcttgtttt ggatgaactt ggcgccgtaa aacctacgga atgggtgtgg
     2581 gatacggtga gccatatcct gaatacgcgc tacaacgaca agcgtacgac cataattacc
     2641 tccaattttg acgatttgcc gccttcttcg aggctggcgg gcgacgaagc catggctgcc
     2701 tcccggcgaa ttgccgctcg ggcggctcga gatgagaccc tgggcgaccg aattggcgag
     2761 cgaatgcgct ctcgattgca cgaaatgtgc cgcaaaatcg agatggaagg cggagatttt
     2821 cggcaaagca ttcggcaggc acactgaaaa agatcgggcc atcgggtgat cgggtcatcg
     2881 gatgaagtaa aaaatcagat cgcccgatga tccgatggcc cgatcacccg atctcggggt
     2941 agtttgcgtg gccttcctcc cctcacaagt ccgcatctca aatacaaaaa aggagcgtat
     3001 ttatggctca tgtaggcact ttgcgggact ttcgctttgc cgataacgcg gatgatgttc
     3061 gcggcgcggc gctttacggg cgcgataacg acaaactggg caagattgat gacgtgattt
     3121 tcgaccatga aaccggcgcg ctgcgctatg cggtggtgga tacgggaggc tggttgagca
     3181 gcaaaaggtt cgttgttccc gcggatcgga ttcgttcgcg cggcgataaa gacgacgaat
     3241 atgcggtaaa cctgacgaaa aagcagattg aaaccttccc cgcctacgac gaaaagcatt
     3301 tagaggacga gaaacgctgg tcggattacg aaaagaatta ccgcaaagcg tcaggattcg
     3361 aggagacggc ggggattttg cacgaagcgg gcggcacaaa tgtgctcatt ccggacgata
     3421 tccccgccac tggaccgcct ccgaccggcc ccggcggcga gcccatctcc ggttacaaga
     3481 gcccaattcg ccatcgcaac accggattga tgaacacaac tcccactggc tggggcgaag
     3541 atcccgataa cgaacgcatg gagcacgtga atgactcgct ggcaaccgat cgcggcgaca
     3601 tcgaggaccg tcaaattgac gaaagcgccg ccgctaaaac ggcgagtcct atgcgtgaag
     3661 tcggcgaagc ggccgatatt tcgcgctcaa ctcgcgaccg ggacatcgcc aatcgcgaga
     3721 aagatcgcac ctatcacacc gatcgcggca gcaaattgtc ggcggaagag cagactatgg
     3781 agggcgattc ggtattcaac agcgccgata ttcaagatcg tatgggcgcg aaacgctcgc
     3841 ccaggtccgc cgatgtgccc aattatgcgg tgattgccgg cggcgaaact cagagttcac
     3901 aggggcgaac gccgaattat ccggacgcaa gtcagggcca gcgctgggcc agatttgaag
     3961 aaaacctgcg ccggcagcgt ccgaatatcg ttgggcgatg cggtatttgc gagggaatgc
     4021 agaacaaaca tcgcgaagac gtcgcgtaaa aggcactggt gttaacacga agggcacgaa
     4081 ggaacttata aggtcacgga gttttttggg ttaaggggcg gtataaacca cagttatcaa
     4141 caaccgcacc ccaaaaaaaa actattgtcc gtgtacttta tctttgcctt cgtgcccttc
     4201 gtgttaaccc caggttcaca tgttttgatg gccggcgcgc atttcctcgt tgaattttgg
     4261 cttgaggagc gcgagcgcgc tgtgcatttt actttcgtat tctccgctgc gcgccacgag
     4321 tttgtcggtt gtggcggcgt attctccgtc tgaatcgagc gtaacgagtc cttcttttgc
     4381 cagctcgcgg gcaatttgcc gcaggtcatc gagcgagcaa tccaggtatt gcgcgtcata
     4441 aggatcggca atccacacgg atttgcgatt tcccaggcgg ccgctgatcc aaaaaatctt
     4501 gctttgcagg aagtcttttc tttgctggtc gctggtgtcg ttgaagataa atcgcttctg
     4561 gcggaagctg taatagcgcg tgctcaccgg aaccggctgg cgattcccgc tcttgagcag
     4621 ctccagttgg ccgagatcga gggttttgcg gatggcgttg taggtcacgc tttccgcgta
     4681 tggctgctcc aatgcaggca aaacctcggc aaacgtgacg gtcaggctgg cggaaacctt
     4741 ggcgtgcagc ggactttccg gctcggcgag ctgcgcaatg ccgtgcagaa tgtaggaatc
     4801 tgctcccgaa gtggaacgat gaaaaggcca tttgaactgc tgcatggcta gcggaaggcc
     4861 atgcagagtg agaaaaacct ccggtcgcgg cgcacgcatg cgcttttcca gcgcccgcaa
     4921 atagctggcg agttcaccgc gcaactcgga acggcgctcg atttcggca
//
LOCUS       DATNDT010000440         6016 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1604295, whole genome shotgun sequence.
ACCESSION   DATNDT010000440 DATNDT010000000
VERSION     DATNDT010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6016
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1604295"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /locus_tag="VN788_14405"
     CDS             <1..1108
                     /locus_tag="VN788_14405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21772.1"
                     /translation="AQRDQSDRLRRLSESQDAWNAAQALAGDRQHLADDLSQLEKQMR
                     DAARDLAGTQQDAASKLRDALGDMDSDDLQTRIQRSADWLRRGYNPNSDSTESGIAKD
                     LDRLGGQVRDAQRALGSGPSPQQQDSQTALDRVQRLKDQMEALTRGLGARNSQNRQQA
                     QLGRDGQRAGQQNGQQGGQQAGQQGGGQQAQQGQAGRGGAFGPGLDRSGGANGRGYRD
                     DGYYTGGQRYGGDGRGNWWIDTGNNSNLPGTAAPPDRSQLPPDPELTYQQSVSNLNQL
                     LRTFQDDPEEKRDLQSLIQEMQRLDPKRFPGNPALLEQIHNQVLNDVNNLELRVQRKL
                     GDQPGQIRSTDPISVPAGYEDAVAEYFRRLSKTP"
     gene            complement(1125..1541)
                     /locus_tag="VN788_14410"
     CDS             complement(1125..1541)
                     /locus_tag="VN788_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21773.1"
                     /translation="MLLLIGVTYGAFWWAGKVPRRPKQVAANAVFLWAPSVGLSAPKR
                     GWWLSCTEDAGQNRCTLSDIKGNTEYEGEFLSSNGKGPVPADQLKIDTRRTLDDKVWA
                     GSVLVPLVFLENGTILIPASKYQEGMHLLKQAQPNH"
     gene            complement(2172..4172)
                     /locus_tag="VN788_14415"
     CDS             complement(2172..4172)
                     /locus_tag="VN788_14415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21774.1"
                     /translation="MSQDTVDIPLREHARAVPPHPHPKSKSRLIKIIAALVIVVSVAG
                     SAAVSRHIEHRLFFSRVALVGIALELAILAFLCRHRVAQILKEFFTATAHPLNLAIFR
                     IAVFWAIFHEVQLSEIGFFSRMPSGLQFAPWGMGMLLPHLPIRARLAAAAAKLLLASS
                     AAGFVGIFSRTSALACALLGFYALGIPQFYGKVDHNQHLLWFALILATSPCGDFFSLD
                     AVIGAWKRAGRGITDPLPPSQVYALPLRFVMLLIGAIYFFPGFWKLWQSGFDWFLTDN
                     LRQQLYLFWTWSFDGSWLPAFRIDQHVLFCRIVAAATPLFELSFIFFMFHRTLRAVAA
                     LDGLVFHAATNRLMRISFLSLRVCYVAFFDWAGIFAYIGRALYREEVFFVYDGNNASL
                     RRLVGFAWVWDVFRRIIYIDSADKEARSRLPLRLPESDERLIAQKTLAAVHNKIWTGP
                     AAYRALAWRIPILWPIAPLLYFWPHSNRVPAVHKGPAAPQPVSTYALPPIAGGRVQLG
                     CIAVVGSLLLLGTVCGGVSRHINGWPFACYPTFSLPPPTQIASLGIVAVAPSGEEIAV
                     TNFGFPYHRYYGLSRNILAIKDPLLRNDRLLLVWARAVRVTPELRTSVAVKFYVENLR
                     IDPALWSRNPENRMLLFIWHPPADGSIPQRHSNVHLLATEWQ"
     gene            complement(4207..5130)
                     /locus_tag="VN788_14420"
     CDS             complement(4207..5130)
                     /locus_tag="VN788_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21775.1"
                     /translation="MLWTITSYFNPLRYANRLRNYRVFRANLVPPLVVVELSPNGEFE
                     LTKNDAEIVVQITGGDVLWQKERLLNLALRYLPDDCNRVAWVDCDVIFQESDWAQRAD
                     QALEQFCLIQPFRERCNLSRGAQISSGIDAACDSISKSVAYKIAMGEAQPEDFGQSDA
                     PLTRNSTAGLAWAARRELLEAHGIYDVCILGSTDRAILCAAMGIFESGVNAVGMRGRQ
                     IEHYLRWAEPFHRAVNGHVGFIDGRVFHLWHGDLKNRKYAERHEGLSRFAFDPFSDIV
                     LDPSGCWRWNGSKPEMHEYVEAYFKSRKEDG"
     gene            complement(5195..5842)
                     /locus_tag="VN788_14425"
     CDS             complement(5195..5842)
                     /locus_tag="VN788_14425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006979910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU21776.1"
                     /translation="MKEWIDALFETKDLVRMGHYQRVEDSNLGLGWLYYALARILRPS
                     RIVVIGSFRGFVPLVFSKALADNGEEGRVWFIDPSFVDDFWKNPSAVRKFFGDFGADN
                     IEHFLVTTQQFVLSEKYRSLAEVGIVFVDGYHSEEQARFDYEAFRDLLSAEGVVLFHD
                     SVRVRTSRMYGPDRLYEHRVKCFVDELKKDSTLQVFDLPFGDGVTLVRRAGNPAA"
BASE COUNT         1438 a         1746 c         1663 g         1169 t
ORIGIN      
        1 tgcgcagcgc gatcagtccg atcgcctgcg ccgcctttcg gaatctcagg atgcctggaa
       61 cgcagcgcaa gctttggccg gggaccgcca gcatttggcc gacgatcttt cgcagctcga
      121 aaagcagatg cgcgatgccg cccgcgatct ggcaggaacc cagcaagacg ccgcctccaa
      181 gctccgcgac gctttgggcg acatggattc ggacgatctg caaacgcgca ttcaaagaag
      241 cgcggactgg cttcgccgcg gctacaatcc aaattcggat tcgaccgaat cgggaattgc
      301 caaagatctc gatcgcctcg gtgggcaggt gcgcgatgct cagcgcgcac tcggttccgg
      361 cccatcgccc cagcagcagg attcgcaaac cgcgctggac cgcgttcagc gcctgaaaga
      421 tcagatggag gccctcacgc gcggcttggg cgcccgcaat tcgcagaatc gccagcaagc
      481 tcagttggga cgcgacggcc agcgcgccgg gcaacaaaat ggtcagcagg gcggtcaaca
      541 agccggtcaa caaggcggtg ggcagcaagc tcagcagggc caagcaggac gcggcggggc
      601 attcgggccg ggcctggacc gctcgggcgg cgccaatggc cgcggctatc gcgatgatgg
      661 ctattacact ggcggacagc gatacggcgg cgacggtcgc ggaaactggt ggatcgatac
      721 gggcaacaac tccaatctgc ccgggactgc cgctcctccc gaccgttccc agcttccacc
      781 tgatccggag ctgacgtatc agcagagcgt cagcaactta aaccagcttc tccgcacttt
      841 ccaggacgat cccgaagaaa agcgcgatct gcaaagcctg attcaggaaa tgcagcgcct
      901 cgatcccaag cgcttccccg gcaatccggc gctgctcgaa caaattcaca atcaagtcct
      961 caacgacgtg aacaacctcg agctgcgagt ccagcgaaag ctcggcgacc agccaggcca
     1021 gattcgcagt accgacccca tctcagtccc cgccggctac gaggacgccg tcgccgaata
     1081 tttccgccgc ctcagcaaaa ccccctagct cgcctcattt ccgcttaatg atttggctga
     1141 gcttgcttca gcaaatgcat gccttcctgg tatttgcttg cgggtatgag aatcgttccg
     1201 ttctccaaga agacgagagg aaccaatacg cttcccgccc acaccttgtc gtccagcgtt
     1261 ctccgcgtat caatctttag ctgatcggcg ggcaccgggc ccttcccatt ggacgaaaga
     1321 aactcgccct catattcagt gttgcccttg atatcgctga gcgtgcagcg attctgcccg
     1381 gcatcctccg tacaggataa ccaccatccc ctttttggcg ccgacagacc aacgctaggg
     1441 gcccatagga acactgcgtt cgcggcaact tgtttcggac ggcgcggcac cttgccagcc
     1501 caccagaaag cgccgtaggt cactccgatc aagaggagca atgttatggt gagtaccgcg
     1561 acaggtgacg caccccttct ccgtgtattt tccacccttg acatcagcgc gcggcgggat
     1621 gatatcacac tagcattagg ccaaagattt gatcgagctt cgtgggtgac acatccttcc
     1681 gcttctgcga agggtgcggg cttgaatgaa acactgactc gaacgtcgct acggtttcgg
     1741 gcaagaacgc cgtggttaca atcgggcgaa cgcttcgtcg aagtccacac caacaaccgc
     1801 ttgcagtcct ccggggaact tgtcatccta atcgtgatgt tgaagggatc cgccctcttg
     1861 acgagggccg gaagtattgc tctttgacaa ttgaataagc acggtcagag gcacatcgga
     1921 cgcaacttct gatgggcgca gcggcgcgtt ctggtgccga ttaggctccc tcgattcgtc
     1981 agaatcgtcg ttttttgcca tgtgccgagc cttcttagcc tcgaaacaac tcgattctaa
     2041 tcgacacttc tgccgagtag aatgtgacgc aactcataga aaataaagga tggcggcaca
     2101 ttctactcga cacatctgct ggaacccacg aattccatgg ctgttgagcc gccgaatcgc
     2161 tgcgctctca actattgcca ctctgtagct agcaagtgca cgttcgaatg tcgctggggg
     2221 atactaccgt ctgcgggcgg atgccaaata aataacaaca tcctgttctc cggattgcgg
     2281 ctccatagag ccgggtcgat ccttaaattc tcgacgtaaa acttcacggc aacagaggtt
     2341 cgaagctccg gggtcacccg gacagcacgc gcccagacaa gcagcagccg atcattccgc
     2401 aggagaggat ccttgattgc gaggatattc ctcgaaaggc cgtagtagcg atgatatgga
     2461 aagccgaagt tcgtcacggc tatctcttct ccggacggtg ccacggcaac aatgcccaga
     2521 gacgcaatct gcgttggagg tggcaaggaa aaggtgggat agcatgcgaa cggccagcca
     2581 ttgatgtgtc tgctcacccc tccgcaaaca gttcccagga gtaaaagact gccgacgaca
     2641 gcaatgcatc ccaattgaac gcggccgcca gcaatcggtg ggagagcgta agtggagacc
     2701 ggctgcggcg cggccggccc tttatgaacg gccggcactc tgttcgaatg cggccagaag
     2761 taaaggagcg gagcaatggg ccataggatc ggaatgcgcc aagcaagcgc gcgataggcc
     2821 gcgggtccag tccagatctt attgtgaacg gcggcgaggg tcttttgcgc gatcaaccgt
     2881 tcgtcacttt cgggcaagcg taagggcagt ctggaacgag cttccttgtc ggcgctatcg
     2941 atgtaaatga tccgccggaa gacatcccac acccatgcga acccgaccag acggcgcaac
     3001 gatgcgttgt tgccgtcgta tacgaaaaag acttcttctc ggtagagtgc gcgcccgatg
     3061 taggcgaaaa tccctgccca atcaaagaaa gcaacgtaac aaactcgcag agacaggaaa
     3121 ctgattctca tcaaccggtt tgtagcagcg tggaaaacta gcccgtcgag ggctgcgacg
     3181 gcgcggagcg ttcggtgaaa cataaagaaa ataaacgaga gttcaaacaa aggtgtggcg
     3241 gcagcaacga tcctgcagaa cagcacgtgt tgatcgatgc ggaaggcagg cagccaactc
     3301 ccgtcgaagg accaagtcca aaagaggtag agctgctggc gcaggttgtc ggtcaggaac
     3361 cagtcgaatc cactctgcca aagtttccag aaacctggaa agaaataaat ggcgccgatc
     3421 agaagcatta cgaagcgaag cggcagtgca tacacttgtg acggcgggag cggatcagtg
     3481 attccgcggc ctgcgcgttt ccacgcgcca atgaccgcgt ccagcgagaa aaagtccccg
     3541 caggggctgg tcgccaggat gagtgcaaac catagcagat gctggttgtg atcgactttt
     3601 ccgtagaact gagggatccc gagagcatag aacccgagta acgcacaggc cagcgcagag
     3661 gtgcgagaga aaattccgac gaatccagca gcactggacg ccagcagcaa cttggcggca
     3721 gctgctgcca atcgggcacg aatcggcaaa tgtggcaaca gcatgcccat gccccagggt
     3781 gcaaattgca atccggaagg catcctgctg aagaatccga tttcagaaag ttggacctcg
     3841 tggaagatcg cccagaaaac cgcgattcta aaaattgcga ggttcagtgg atgtgctgtg
     3901 gccgtgaaaa actctttcag gatttgcgcc acgcggtggc ggcacaaaaa cgcgagaatc
     3961 gcgagttcga gtgcgattcc gaccagtgca actcgcgaga aaaacagtct atgctcgata
     4021 tgccgagaga cggccgcaga tccggcgaca cttaccacaa taacaagcgc ggcaattatt
     4081 ttgatcagtc ggcttttgct ctttggatgt ggatgcggag gaacagcccg ggcgtgctca
     4141 cgcaacggaa tatcaactgt gtcctggctc atgacaaact cggcgaggcc gcagacccgt
     4201 atcggcttaa ccgtcttcct ttcgggattt gaaataggct tccacatact cgtgcatttc
     4261 cggtttgctc ccgttccatc gccagcagcc gctcgggtct aagacgatat cgctgaacgg
     4321 atcgaacgcg aatctgctca atccttcgtg gcgctcggca tacttgcgat ttttcaagtc
     4381 gccatgccag aggtgaaaga cgcgcccatc gataaaaccg acgtgcccat tcacggcgcg
     4441 atgaaacggc tccgcccagc gcagataatg ctcgatctga cggccgcgca ttcccacggc
     4501 gttcacaccg ctttcgaaga tgcccattgc ggcacacaaa atggcgcggt ccgtggatcc
     4561 gagaatgcaa acgtcgtaga tcccatgcgc ttccagaagt tcgcggcggg cggcccaagc
     4621 aagccctgcg gtggagtttc gcgtgagcgg cgcatcagat tgaccaaagt cttccggctg
     4681 cgcttcaccc attgcgattt tgtaggcaac ggatttgctg atcgaatcgc acgcagcatc
     4741 gattcctgaa gagatctgcg cgccgcgaga gagattgcag cgctcgcgaa agggttgaat
     4801 cagacagaat tgttccagag cctgatctgc gcgctgggcc cagtcagact cctggaagat
     4861 tacgtcacag tctacccaag cgacgcggtt gcaatcatcc ggcaagtaac ggagcgccag
     4921 gttcagtaag cgttccttct gccacagcac gtctccgccg gtaatctgca ctacgatttc
     4981 agcgtcgttt ttggtcagct caaattctcc gttgggcgat aactccacaa cgaccagcgg
     5041 aggcacgagg ttcgctctga aaacccgata attgcgcagg cggtttgcgt atcggagtgg
     5101 attgaaatac gaagtgatgg tccacagcat gctcggtatc acctcgccat aacttcgttc
     5161 cgaaaaggcc cagacaggag cgttggccgc tccatcacgc tgccggattt ccggctcttc
     5221 gaacgagtgt taccccgtcg ccaaacggca ggtcaaaaac ctgcaaggtg ctgtccttct
     5281 ttagctcatc gacgaaacac ttgacgcgat gttcgtagag ccggtccggc ccatacatcc
     5341 gcgaagtgcg gactctcacg ctatcgtgga acaggacgac cccttcggcc gagagaaggt
     5401 ctcgaaaagc ctcgtaatca aagcgagctt gctcctccga atggtaacca tcgacaaaaa
     5461 cgataccgac ttccgcaagc gaccgatact tctcagacag tacaaattgc tgggtcgtca
     5521 ccaggaagtg ctcgatgttg tccgccccga aatctccgaa gaatttgcga accgccgaag
     5581 gatttttcca gaaatcatca acgaaggatg ggtcaatgaa ccagactcgg ccttcctcgc
     5641 cgttgtctgc cagcgccttg ctgaatacca atggcacaaa tccacggaaa gaaccgatta
     5701 ccacgattct cgagggacga agaattctcg cgagcgcata atagagccag cccaaaccga
     5761 ggtttgagtc ctccaccctc tggtagtgtc ccatgcggac gagatctttg gtttcaaaga
     5821 gcgcatcgat ccattccttc atgaggtttc agctccgact aaccttgacc agacgcctgc
     5881 cgaaaggatc gcggtaatat ggcacatctt gactggatat gcccagtgta gtgcggttcg
     5941 gtggcggtcg cggagtcgag acatcctcgt gctgtcaatt aagaaacatc cgcctcacta
     6001 tacttctcgg aagacg
//
LOCUS       DATNDT010000441         9617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_405464, whole genome shotgun sequence.
ACCESSION   DATNDT010000441 DATNDT010000000
VERSION     DATNDT010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9617
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_405464"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..325
                     /locus_tag="VN788_14430"
     CDS             <1..325
                     /locus_tag="VN788_14430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21777.1"
                     /translation="LALATAGVHGCIPVFWSMPGLYLSGTAAAGGIALISTIGNLAGA
                     VGPVLLGYAKSQTGTFNSGLYAMSGLLVIAGMLVLTTLSRGRFGPNAAIPVSQDFAAS
                     GKLPT"
     gene            complement(595..4197)
                     /locus_tag="VN788_14435"
     CDS             complement(595..4197)
                     /locus_tag="VN788_14435"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU21778.1"
                     /translation="MKARLASVMVIFLFFTLVSVLALNAQTASTGALTVVVTDPSGAA
                     ILGAEATITSSAGESRTLKTQVDGTAKFVLLNVGNYKVTISDQGFKTVTVPSVSVGVA
                     ETHVLTQSLELGATTQQVTVNGNVEVTQTESSAVGSTVNQRTLSEVPLATRNFTQIMN
                     LSSGVTSNVNDATTLGRGNQNIVVNGTSDISSNLQMDGFSITGFMTGGTTDPFGGLWG
                     TPPIPSPDALQEFKIQTSDYDAGYGHSGGANVNIVTKSGTNDLHGSAFEFVRNNMFDA
                     NGFFQNLKGSRRGELKQNQYGGTIGGPILKDKLFAFFSYQGTRQVNGVASSGFSNLIL
                     PGALTDDRSATTLQTEFCTLGPQNQTPGTDQYIESHAFLYSPTKLTNPSLDAIACPGG
                     PGQPINPVALGLLQAKLPDGSYVIPSPTSVVNGVGHALFSVPATFQEDQGLLNFDYVI
                     SPKHTLSEKTFYTFGLQDNTFQGTQPPGGSGTNLTGSELSTTKLTSILSNTLVNEVHF
                     SYLNTREILRGHFPLTTTQFGVTGAAPYWTVFPQVTITGLFGFGGAYTDTGNNPQVGY
                     EVGDQLSWNHGRHTIRLGYGEEYNELNLKIFGRTRGTLTFQTWEDFLLGLTAAQNGTT
                     CNGCGDQSNIASGTATIQGPPPGGTQGDVRQNYLYSFIQDDFKMSPRLTWNLGLRWEY
                     NGNPYDTVPANDQVNPSFAQFLANAVPPAGGTYAGFTAGTVIGTPLPPGIPQRKNRLY
                     TEGHSPFTNFAPRIGFAWQPFSSSGKLVVRGSGGIFYIAGTAGFISNVTATDEPQAYP
                     LSRSGTSNDTASWSCPFCPVVPIGFVQRFQNSAVSAGSAVDPNMRAATMTSESLNVQY
                     AIKSSLSLEVGYVGNRVEHIIVLGNPQNIPLFATATQPVNCSHPTGCIATNGSSGPTG
                     PAARTPVMGLVPFGMSDYASVGDSEYSSLQTTLRKTFSHGLQFQAAYTYGRTFTDVRG
                     ASWVAGGSFTSNDPTNRRQLHGPADFDRPQRLVVSYTYQLPDFEGSKGFTGKALSGWG
                     LSGVTIVQSGQSYSLTDPRGGTAYGGSTSRAQTCAAVPQSVRNQIYAPKGFHAAAATG
                     TSAFVPASSLFCVPPVVANPTPGQSPRGFGNTPPGFAVGPGQFNWDMSITKKTVVGGI
                     NENAYLEFRSEFFNAFNRTELGNPSGSVTSASFGQITTTNVGPRIIQLALKYVF"
     gene            complement(4773..4957)
                     /locus_tag="VN788_14440"
                     /pseudo
     CDS             complement(4773..4957)
                     /locus_tag="VN788_14440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158541.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
     gene            complement(4978..6225)
                     /locus_tag="VN788_14445"
     CDS             complement(4978..6225)
                     /locus_tag="VN788_14445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007644196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU21779.1"
                     /translation="MKSVQLTERNLAAIKWLTFLMFMMFAMTTDAVGVIIPQVIKEFH
                     LSMTVGGAFHYSTMSGIAFAGIALGYLADRLGRKKTIILGLVLFALNSYLFAVGKSFL
                     VFLLLLLISGTAIGIFKTGALALIGDITRSTTEHTATMNVVEGFFAVGAIMGPAIVAH
                     FLAIGASWKWLYVIAGSICVVLIVTASSVTYPRSKHSVEAVDLKRTMRMMKNPYALGF
                     SAVIVLYVAVESAVYVWMPTLLSAYHGSAVFVATYAISIFFVLRAAGRFSGAWVLARL
                     NWTTVAALCSFAILACFVLSMIGGISTSVFLLPLSGLFMSVLYPTINSKGISCFRKAE
                     HGAIAGVILFFTCGAAVVGPLAMGAISDVLGDPTYGFILATGFAGLLFVSLLVNWIAN
                     PARQILHELDATEYHVAEIYGHK"
     gene            complement(6225..7733)
                     /gene="gtfA"
                     /locus_tag="VN788_14450"
     CDS             complement(6225..7733)
                     /gene="gtfA"
                     /locus_tag="VN788_14450"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HXU21780.1"
                     /translation="MKNQVQLIAYADRLAGDLEQLKALLRGALKGIFGGVHLLPFFVP
                     IDGADAGFDPVDHTEVDPRLGDWDDVRALTEDVAMMADLIVNHISRDSPQFQDFIRNG
                     ANSSFAGMFLTYDRIFPLGAREQDLVRIYRPRPGLPFSSVTLQSSATRLVWTTFTPRQ
                     VDIDVQHPQGRAYLSAILNRFQSAGIRMVRLDAVGYAIKKAGTSCFMIPDTYSFIESL
                     ASEARALGIEILVEIHSHYLQQVEIARRVDWVYDFALPPLVLHSLFTRDTRALGDWLR
                     VRPKNAVTVLDTHDGIGVIDVGTGRDGQPGLLTPEEIDRFVDTIHSRSGGQSRLATGA
                     AANNLDLYQVNCTFYDALGRRGNEYLVARAIQFFVPGIPQVYYVGLLAGINDMELLAR
                     TQVGRDINRHYYTLSELQDALANPVVRNLLDLIRLRNTHPAFAGQPQFETPSDQVLVI
                     TWSVGAHWTKLEVDFSEPRALVTYSNTSAANENESATGRWESCSASSAREKI"
     gene            7892..9010
                     /locus_tag="VN788_14455"
     CDS             7892..9010
                     /locus_tag="VN788_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013572900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXU21781.1"
                     /translation="MSNHKYRQIFEKLQDDICSGHYKPGSRLPSESELVRRFEVSRMT
                     VFHAMRELQTLGLIVRRVGSGTFVSSNAHSGSHIFGLLIPELGQTEIFEAICQGMMEA
                     QEAMHHSLLWGNSVSREQDTEKAAEQLCEHYISRKVSGVFFAPVEYSTNRFQSNHRIV
                     AALDRAGIPVVLLDRCLEPYPRRSKYDLVGIDNRRAGYVATEHLIRADARRIVFFARP
                     NSAPTVDARIAGYREALQSHGGDSGRDLVRIGEASDAKFVKSILKKDRPHAFVCANDL
                     TAGDLMHTLISLGERIPKDIRIVGIDDVKYARLLPVPLTTLHQPCRDLGRMALTVMLD
                     RIANPDLPPRDVLLRCELIVRKSCGMLSKMDQRQARSL"
     gene            9363..>9617
                     /locus_tag="VN788_14460"
     CDS             9363..>9617
                     /locus_tag="VN788_14460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU21782.1"
                     /translation="MRNVLGSLLAFGRAVNGRRLIARVAAATFAFAATATFCTPLNAQ
                     VTFGSIIGTVSDPSGATVTGATVKLTNSGTSETRSVQTDSG"
BASE COUNT         2211 a         2574 c         2721 g         2111 t
ORIGIN      
        1 cctcgcgctt gccacagcgg gcgtgcacgg ctgcattcca gtgttttggt cgatgccggg
       61 cctctatctc tcgggaacag ctgccgccgg cgggatcgca ctgatcagca ccatcggaaa
      121 tctcgcagga gccgtcggtc ctgtcctgct gggttacgcc aagagccaaa ccggaacatt
      181 caacagcggt ttgtacgcca tgagtggtct attggtcatc gcagggatgt tagtgctgac
      241 gacgctctct cggggacggt tcgggccaaa cgctgccatc cctgtcagcc aggactttgc
      301 ggcgagcgga aaattgccca catagcactg accaagctca tccgcggacg gcgtcccatc
      361 atccggaagg atgtcccggg cgcgttaggg ccgtggctga ggacgtgggc tcaccaccag
      421 ccgagggtgt ccacgacata cacgagggag aagccctttt cgcctttatt gctctttctg
      481 ccacttagtc ttttgacaca aggcgtgctg cgacaaatgg tcacttgcgt cgtagggtgg
      541 atccaccagt tcgtaacctc atctctgcac ggccacgaac tggcgaatag atgttcagaa
      601 tacgtatttc agcgccagct ggattatgcg tggtccaacg ttcgtggtgg tgatttgacc
      661 aaatgaagct gaagtaacgc tcccagacgg attcccgagt tcggtgcggt tgaaagcatt
      721 gaagaactca gagcggaatt ccagataggc attctcattg attccaccca ccacggtctt
      781 ttttgtgatc gacatatccc agttgaattg tcccgggcca actgcaaagc ccggaggggt
      841 attgccaaac ccccttgggc tttggcctgg agtgggattg gctactactg gcggtacgca
      901 aaagagagag ctggcgggga caaaggccga agtaccggtg gccgcagcag catgaaatcc
      961 cttcggcgca tagatctgat ttctcacgga ttgagggact gcagcgcacg tctgggcgcg
     1021 cgaggttgag cctccatagg ccgtccctcc cctcggatca gttagcgaat aggactgacc
     1081 gctttgaact attgtcacgc ctgatagtcc ccacccggac agagctttac ctgtgaagcc
     1141 cttcgagcct tcgaagtcgg gaagttgata ggtgtagctc accacgaggc gttggggccg
     1201 gtcgaaatcg gcaggcccgt gcagctgacg gcggtttgta ggatcgttgc tggtaaagct
     1261 gcccccagct acccaactcg cgcctcggac gtccgtgaac gttcgaccat aggtataagc
     1321 ggcctggaac tgcagaccgt gggagaatgt ttttcgcagc gttgtttgca gcgatgagta
     1381 ttcggaatct ccaacgctgg cgtaatcgct cattccaaaa ggtactaaac ccattaccgg
     1441 tgttcgagcc gctggacctg tcggtccgga ggatccgttg gtcgcgatgc agccggtagg
     1501 atggctgcag ttcaccggct gggttgcggt agcgaataga gggatattct ggggattgcc
     1561 caagacgatg atgtgctcca ctctgttacc tacgtagccc acctcaagtg acagactgct
     1621 ctttatcgca tactggacgt taagagactc actcgtcatc gtcgcggctc gcatgttcgg
     1681 atcgaccgca cttccagcgc taaccgcgga gttctgaaag cgctgcacaa aacctatcgg
     1741 gacgactggg cagaaggggc aagaccagct cgccgtatca tttgatgtac cactccgaga
     1801 cagcgggtag gcttgaggct cgtcggttgc cgtcacgttg ctgatgaagc ctgcagttcc
     1861 ggctatatag aatattccac ctgagccacg cacaacgagc ttcccgctgc tgctaaacgg
     1921 ctgccatgca aagccgattc gcggggcaaa attcgtgaaa gggctgtggc cctcagtgta
     1981 gagcctgttc ttgcgctggg gaattccggg tggcagcggt gttccgatga ctgtccccgc
     2041 cgtgaaaccc gcgtaagtcc cgccggcggg tggaacggca ttcgccaaaa attgtgcgaa
     2101 ggacggattg acttgatcgt ttgccggcac agtgtcgtat ggattgccgt tgtactccca
     2161 acgaagaccc aaattccagg tgagccgcgg gctcatcttg aaatcgtctt ggatgaacga
     2221 gtaaagataa ttttggcgaa cgtcgccttg agtgccgcca ggcggcggcc cttgtatggt
     2281 agcggttcca ctggcgatgt tgctttgatc cccgcagcca ttgcaggtag tgccattttg
     2341 ggcagcggtc agcccgagca ggaaatcttc ccaagtttgg aatgtcagag ttccccgggt
     2401 tcgcccgaag atcttgagat tgagttcgtt gtattcttcg ccgtaaccca ggcggatcgt
     2461 gtggcgccca tgattccagg agagttggtc tcccacttcg taacctacct gcgggttgtt
     2521 gcccgtatcg gtgtatgcgc ctccaaaacc gaaaaggccg gtaatcgtaa cctgggggaa
     2581 cacggtccaa tacggagccg cacctgtcac cccgaactgt gtcgttgtga gaggaaaatg
     2641 tccgcgcaga atttcacgcg tgttcaaata agaaaagtga acttcgttca ccagagtgtt
     2701 tgaaagtatc gaagtcagct tcgtcgtgga gagttcgctt cccgtaagat tcgtacccga
     2761 gccccccggc ggctgcgtac cctgaaaagt gttgtcctgg agtccaaagg tatagaaggt
     2821 tttttcagac agtgtgtgct tcggcgaaat cacgtagtcg aaattgagca agccttgatc
     2881 ttcttgaaat gttgcaggga ccgaaaacag ggcatggccc accccgttca caaccgacgt
     2941 tgggctgggt ataacatacg agccatcggg cagttttgct tgcagaagtc caagggcgac
     3001 tggattgatg ggctggccgg gcccaccggg acaggcgatg gcgtctagtg acggatttgt
     3061 gagctttgta ggggaataaa gaaacgcgtg agactcaata tattggtcag ttcccggcgt
     3121 ctgattttgg gggccgagag tgcagaactc ggtttgaagg gtcgttgccg atctgtcatc
     3181 cgtaagcgct ccgggcagaa ttagattgga aaaacccgaa ctggcaaccc cgttcacttg
     3241 ccgggtacct tgataggaaa agaaggcgaa cagcttgtcc tttaggatcg gaccgccgat
     3301 ggtgccgcca tactggttct gttttagctc tccgcgccgt gaccccttca gattttgaaa
     3361 gaagccgttg gcatcgaaca tattgttgcg aacgaattca aaggctgatc catgaaggtc
     3421 attggtgccc gacttcgtga cgatgttcac gttcgcgccg ccgctgtgcc catacccagc
     3481 gtcatagtcc gaagtctgaa tcttgaactc ctgcagtgca tcggggctgg gaatcggcgg
     3541 agtaccccag aggccgccga acggatcggt cgttcctcca gtcatgaagc cagtgatgga
     3601 aaacccatcc atctgaagat tgctgctgat atctgaagtg ccattcacga cgatattttg
     3661 attgccgcgg cctagcgtgg tggcatcatt gacattcgag gtcacgcctg aagacaaatt
     3721 cataatctgc gtgaagttgc gcgtagccaa aggcacctca ctgagcgtcc gctggttgac
     3781 ggtgcttccc acagcggaac tctccgtttg ggtcacctcg acgtttccat tcactgtcac
     3841 ctgttgtgtc gttgccccaa gctctagcga ttgagtcagc acgtgtgttt cggcaacgcc
     3901 tacggacaca gatggcaccg tgacggtctt gaaaccctga tcgctgattg tgaccttgta
     3961 gttgccgacg ttcagaagca cgaatttggc agtgccgtct acttgcgtct tcagggtgcg
     4021 cgattcgccg gcggaacttg taatcgttgc ctccgctcca agaatcgccg caccgctggg
     4081 gtcagtgacc acgaccgtca aggctccagt gctggccgtt tgcgcgttga gggccaatac
     4141 ggacactaga gtgaagaaaa ggaagataac cattacagat gcaagtctcg ccttcatttt
     4201 tctccctcca caacccgttc gtaaaaactg accgcagtac caagccgccg ttgggggcga
     4261 tcggaattta caggtgttgt cgccccacgg ccaccctatc cgcgaatcag aaataaggct
     4321 aagacttcgc gcgtttaaga tgcctcgcgt tttcctaccc gtcgcgagcg catttcacaa
     4381 tgcgagatgc caagactcgt atggaaacga cgggtttcta acacggggtg ccttggctgt
     4441 caaggaaaaa cagggtgtat atcggtgcga atgatgaatg agcagtgcag cattaatcgg
     4501 gaactgctag cccataaact gcttggcgat cgatgaactt ctcatagtta gaaattgcaa
     4561 tctagtgagt cgcggctcgc aatttgtttt tgtggagggc agcaccgcgc aaggatgtca
     4621 cctccctcga acacatcgga tttagagtgg agtcacacat attccccgac gtagtaagcc
     4681 ttagaggctc ttgccaaaac taatgtcgct gacgaccatt ggagcaagtc tcaggccacc
     4741 gctcggcatc cccttaactc ttcggtccac ctgctaacca atccggcgag ggtcgagaca
     4801 gcaacatatc gccaagtgct cgtcgccgat ggcgggatcg ctcctatcat tacatctcgg
     4861 gcgattcaaa ggacgaaacc agagcgccga aaatatcgaa cctgtgtttt actccggccg
     4921 tcgcgccgct aagcgcgaaa acaaatgtgc cacccaggtc gaagccagga gaggaactta
     4981 cttatgacca tatatttcag ccacgtggta ttcagtcgca tccagctcgt gcagaatctg
     5041 gcgagcaggg ttagcaatcc agttcaccaa gagcgagacg aaaagcaggc cagcaaatcc
     5101 agtggccaga atgaatccgt acgtcgggtc gccaagaacg tcactgatcg cgcccatggc
     5161 caggggcccg acgaccgccg ccccgcaggt aaaaaataaa atcacgccgg cgatcgctcc
     5221 gtgttcagcc ttgcggaagc agctgatccc tttggagttg atcgtgggat aaaggaccga
     5281 catgaacaag cccgagaggg gaaggaggaa caccgacgtc gagattccgc cgatcatact
     5341 caacacgaaa caggcaagta tcgcgaaact gcaaagcgcg gcaacagtgg tccagttcaa
     5401 cctggcgagc acccatgcac ctgaaaacct ccccgccgcg cgaagcacaa agaagatcga
     5461 aatggcgtac gttgcaacga aaactgcgga tccgtgatag gcggaaagca gggtaggcat
     5521 ccagacgtaa accgcgcttt cgacggcgac gtagagaact atgacagcgg aaaaaccgag
     5581 cgcataagga ttcttcatca tgcgcatggt gcgtttcaag tctacagctt cgaccgaatg
     5641 tttggatcgc ggataggtca ccgaggaggc ggttactatg aggacgacgc aaatacttcc
     5701 tgctatgaca tacaaccact tccacgaagc tccgatcgcc aagaagtgag cgactatggc
     5761 agggcccata attgcaccga ctgcgaagaa gccttctacc acattcatag tggctgtgtg
     5821 ttcagtcgtc gatctcgtga tgtcgccgat gagggccagc gcgcccgttt tgaaaatgcc
     5881 gattgccgta ccggaaataa gtagcagcag aaggaaaacc aagaaagact ttccgacagc
     5941 gaacaggtag gagttgagag caaacaggac gaggccgagg ataatggttt tcttccgccc
     6001 aagtctgtcc gccaagtagc cgagagcaat gccggcgaac gcgattccag acatagtgga
     6061 gtaatgaaaa gctccgccca cggtcatgct aaggtgaaat tccttgatga cctgcggaat
     6121 gatgacgccg accgcgtccg tggtcattgc gaacatcatg aacatcaaga acgtaagcca
     6181 tttgatggcc gcgaggtttc tttcggtcag ttggacactc ttcatcagat tttctcacga
     6241 gccgatgatg ccgaacacga ttcccatctt ccggtggctg attcattttc gttcgcggca
     6301 cttgtgttgg agtaggtaac caaggcgcgc ggctctgaga agtccacctc gagtttcgtc
     6361 cagtgcgcac cgacactcca cgtgattacc aacacttgat cggatggagt ttcgaactgc
     6421 ggttgcccgg caaaagcggg atgtgtattg cgtagacgga tcagatcgag gagattccgc
     6481 acgaccgggt tggccagcgc atcctgaagt tcggatagcg tgtagtagtg gcgatttatg
     6541 tcgcggccaa cttgagtgcg ggccaggagc tccatgtcgt ttatcccggc gagtaatccc
     6601 acgtaataaa cttggggaat tcccggcacg aagaactgga tggcgcgcgc gacgagatat
     6661 tcatttcctc tgcgtcccag ggcgtcgtag aaggtgcaat tcacctggta gaggtcgaga
     6721 ttgttcgccg ctgcaccggt cgcaagccgg ctctggccgc cactccgtga atggatcgtg
     6781 tcaacgaacc tgtcgatctc ttccggcgtt agaaggcccg gttggccgtc acggcccgtg
     6841 ccgacatcaa tgacgccgat accatcgtga gtgtcgagca ctgtgactgc attcttaggg
     6901 cgaactcgca gccagtcgcc aagggctcga gtgtcgcggg taaaaagtga atgcaggacg
     6961 agtggcggaa gtgcgaaatc gtagacccaa tcgactcgtc gcgcaatctc gacctgttgc
     7021 aagtagtggc tgtggatctc cacaagaatt tcgattccca aggcccgagc ctctgatgca
     7081 agagactcaa tgaaagaata cgtgtccggg atcataaagc agctggttcc ggccttcttg
     7141 attgcgtagc caaccgcatc aagccgaacc atgcgtatcc cggcagattg aaatcggtta
     7201 aggatggcgc tcagataagc cctgccttgg ggatgctgca catcgatgtc gacctggcgg
     7261 ggagtgaagg tggtccacac aagccgtgtc gcactcgatt gcagcgttac gctcgaaaac
     7321 ggcaaaccag gacgcggacg atagatccgc actagatctt gctcgcgcgc cccgaggggg
     7381 aaaatgcgat cgtaggtgag aaacataccc gcgaatgacg aattggcgcc atttctgatg
     7441 aagtcttgga actgcggaga gtcgcgcgat atgtgattga cgatgagatc cgccatcatc
     7501 gcaacgtctt cagtgagtgc tcgaacgtca tcccaatcgc cgagccgagg gtcaacctct
     7561 gtgtgatcaa ctgggtcgaa gccggcgtcg gcaccgtcga tcggtacaaa aaaaggcaac
     7621 aggtgcacac cgccgaaaat gcctttgaga gcgccgcgga gaagcgcctt cagttgctct
     7681 agatcgccgg ctaaccggtc ggcgtaggcg atcagttgga cttgattctt caagctcttc
     7741 cttgctcttc gacttagcca ccgagagcac ttctaaagcc caactggtca acctgtcaag
     7801 gacattccgg tacttcggcc tgcaagttgt ctatggtaat atttcgagcc atccgcacag
     7861 ttgtgcgagc ggcaagaaca aaggggtact catgtcgaac cacaagtacc ggcagatttt
     7921 cgagaaactt caggacgaca tttgctcggg ccattacaag cctggcagcc gtttgccgag
     7981 cgaatctgaa ctcgtcagac gctttgaggt gtcgcggatg acagtgtttc atgcgatgcg
     8041 tgagctgcaa acgctgggcc taatagtccg gcgggttggt tctggaacgt tcgtatcctc
     8101 gaatgcacac tcaggcagcc atatttttgg gctactgatc ccagagttgg ggcagacgga
     8161 aattttcgaa gcgatctgcc aaggcatgat ggaagcacag gaggcgatgc atcattccct
     8221 cctgtgggga aactcagtat cgagggaaca ggacacagag aaggcggctg agcaactctg
     8281 cgaacactac atttcacgaa aggtatcggg cgtattcttt gcccctgtcg agtacagcac
     8341 gaatagattc cagtcaaacc atcggatcgt tgctgccctt gatcgagccg gcatccccgt
     8401 ggttcttctt gaccgatgcc tcgaacccta tcctaggcgc agcaagtatg atttggttgg
     8461 catcgataac cgccgcgctg gatacgtggc caccgaacac ttgattaggg ctgacgccag
     8521 gcggatcgtt ttctttgcga ggcctaattc cgcccctaca gtagacgccc gcatcgccgg
     8581 atatcgggag gcgctacaat cccacggagg cgattccggt cgcgatctgg tccggatcgg
     8641 agaggcctcg gacgcgaagt tcgtcaaatc aatactgaaa aaagacaggc cgcacgcttt
     8701 cgtatgcgcc aacgacctga cggctggaga tttgatgcat acgctcatat ccctgggcga
     8761 gcgcattccc aaagacattc gcatcgtggg aattgacgat gtgaaatatg cccggttatt
     8821 gccagttcct ctcaccacgc tccaccagcc gtgtcgcgac cttgggagaa tggccctgac
     8881 agtaatgctg gacaggattg caaaccccga tttgcctccg cgcgacgtcc tgctgagatg
     8941 cgaactgatc gtgcgcaaat cttgtgggat gctctctaaa atggatcagc gccaagcgag
     9001 atcgctttag ccgcacgttt ggagcacacg tacggggcgg ataattcgtt cttcttcatc
     9061 ataacccctc ctccttcgag cagatattat gcaggctccc tggcagaccg cagaagtcgt
     9121 ctatacattt cagggtgcac tatgcgcagt atagacaact ttttgcatag ttgtctgcaa
     9181 agtgtcttga cagcttagag tgcctggagt tacaagcctg ctccagtaat ggcttggtgg
     9241 agcgcctttg tcgcaactgc ggcgttccgg tcaacgagag ccaagaatcg gattgggtct
     9301 ccgaatcgtc agctttcaca aatcaatcgt tcgtcgtttg tggttttgag ggagcgataa
     9361 atatgcgtaa cgttctgggg tctctcctgg cttttggacg agcagtgaat gggcggcgtc
     9421 taattgcgcg agtggcggca gcgacctttg ccttcgctgc aacagccact ttctgcacac
     9481 cgttgaatgc ccaggttacg ttcggatcga taataggaac agtttccgat ccgagcgggg
     9541 caaccgtcac cggcgccacg gtgaaactga ccaattcagg cacgagcgaa acgaggagcg
     9601 tgcagacgga ttcaggc
//
LOCUS       DATNDT010000442         3735 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_997421, whole genome shotgun sequence.
ACCESSION   DATNDT010000442 DATNDT010000000
VERSION     DATNDT010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997421"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2402)
                     /locus_tag="VN788_14465"
     CDS             complement(<1..2402)
                     /locus_tag="VN788_14465"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU21783.1"
                     /translation="MKNTCTRLRHFANLAISYAICALAVACAIGVIVTPLSAQTVDTV
                     ILGTITDPSGAAIPGAQVTVTSPSTGVSHTVTTGANGAYRVGYLLPGAYTVSAQAQGF
                     SRAVRSNITLELSQQAEVDIPLAIGQTTQSVEVTAAAPLLNTTSGALSGVVNPQETVG
                     LPLNGRNFGQLAVLTPGVQASASSQNTSFSANGIDSEHEEVTLDGGSIVDNRANSVFL
                     LPSIDAIQEFRVNTSNYAAEYGNNSGAVVSVQLKSGTNDFHGVAYDFLRNDALDARGY
                     FLSPTAKKNPLRRNQFGGILGGPIKKNGTFFLIGYEGNRSFQGSPSQSETFTPQELSG
                     DFSFLSTPLVNPRTGNTITNNDISGLLDPNAVRIAQTYMPAANCNGEKSGAINYCGES
                     ISTVDSDQGLIRVDQTVGNNGHFFADYVGNFGHFPSVSLNPFFPVRYDFRAQNFTSQY
                     VHTFSPAVVNETLFSVNYGTLNQLSPRAATGFSISKTLGINGFDTEGPNGPPIQQQFQ
                     GFPQLSISSFLAMGDNDGGQAEDQSKTYQFVDNVSISHGRHFVKFGGELRKVDDNADT
                     SNSSWGNETFNGGLSGFGAADFLMGLPDTTLSPEGIPVNAIRQWRSALYIQDDWTATS
                     RLTLNLGVRWDWTQVPQDTAGVSRTLRFDLPGGPVLWPAPGDSQSMYIHPQPPIEPRF
                     GFAYKIDSKDVLRGGFGLFDAEAKFDNINILQLNPPLHASVESINPLQSGGLVTLENP
                     IDQNEVAAGIFDVVSEPPDRIKRDPYQTDWNLTFQRQLTNSSVFEIGYNATRGTDLDT
                     SDL"
     gene            complement(2629..2976)
                     /locus_tag="VN788_14470"
     CDS             complement(2629..2976)
                     /locus_tag="VN788_14470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21784.1"
                     /translation="MAMDEICTAKDRHGRRPHSGRDLRKRKRLKLSLAAHLRPFDPRL
                     RDLEDIPLVVDFNRDGLHFITLMLHYFVGMKLEVTFPHGHNVTSQRKYIGSVVRIDDR
                     GDGRSGISVKFLF"
     gene            3054..3731
                     /locus_tag="VN788_14475"
     CDS             3054..3731
                     /locus_tag="VN788_14475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXU21785.1"
                     /translation="MTLLNFRAAIEGVDFADISAFREEHMSSSRGLLGCALLIAFSVA
                     PACAQAPGGQAPARGRGPAGPGLTLTSPDFPDGGVIPAKFTQADPKPVSPELDWTNVP
                     QGTVSFVLLLHDPDTALQRKSDDVLHWLVFNIPGTAHQLAQGQPADAQLPDGTIQAKN
                     LRGGVGFMGPGAPAAGPDHHYTFELFALDEKLDLGPDATRAQVMDAIQGHILGKGVLV
                     GRFHRPQ"
BASE COUNT          799 a          969 c         1140 g          827 t
ORIGIN      
        1 aggtcgctgg tatcgaggtc tgtgccccgc gtggcgttgt agccgatctc gaagacgctg
       61 ctgttggtaa gttgcctctg gaatgtgagg ttccagtccg tctggtaggg atctctcttg
      121 atgcgatcgg gcggctctga cacgacgtcg aagatgccag cagcaacttc gttctgatcg
      181 atcggatttt ccagcgtgac aagcccaccc gattgcagcg gattgatgga ttccacgctg
      241 gcgtggagcg gcggattcag ttgaagaata ttgatgttgt cgaacttagc ctctgcatcg
      301 aagaggccga aaccgccgcg gagcacgtcc ttgctatcga tcttgtaagc aaatccaaag
      361 cgaggctcga ttggcggctg cggatggata tacatcgact gcgagtcgcc gggagcgggc
      421 caaagaacgg gtccaccggg tagatcgaac cttagtgtgc ggctcacgcc ggctgtgtcc
      481 tgcggcactt gcgtccagtc ccaacgcacg ccgagattga gcgtcaaacg cgaagtggcc
      541 gtccaatcgt cctgtatata caacgcagac cgccattgcc ggatagcgtt gacgggaatg
      601 ccttcgggac tcaacgtggt atccggcaaa cccatcagaa aatcggcagc gccgaaaccg
      661 ctcaaaccgc cgttgaaggt ctcatttccc caggaagaat ttgaggtgtc ggcattatcg
      721 tcgacctttc gcagctcacc accgaatttg acgaaatgcc ttccatggga aatgctgacg
      781 ttatcgacaa attgataggt tttactttga tcctcggctt ggccgccatc gttatcgccc
      841 attgcgagaa aactgctgat acttagttga gggaagccct ggaactgctg ctggataggc
      901 ggcccattgg gaccttcggt atcgaagcca ttgatgccca gcgtcttgga tatggaaaac
      961 cctgtcgccg cccgcggact cagttggttc agtgtgccgt aattgaccga gaaaagcgtt
     1021 tcattaacga cggcgggaga gaacgtgtgc acgtactgcg aagtgaagtt ttgggcgcgg
     1081 aagtcgtacc taaccgggaa aaacggattc agtgacacgg atgggaaatg gccgaaattt
     1141 cccacgtaat ccgcgaagaa gtgcccgttg tttcccacgg tttgatcaac gcggatgagg
     1201 ccctggtccg aatcaaccgt actgattgac tcgccgcaat agttgatggc tccgctcttc
     1261 tcgccgttgc agttggcggc gggcatgtag gtttgagcga tcctgacggc attaggatcc
     1321 aagagtccgg aaatatcgtt attcgttatc gtgttaccgg tacgcggatt gaccagaggg
     1381 gtcgataaga aactgaagtc gccgctgagt tcttggggcg tgaaggtctc cgactgcgac
     1441 gggctgccct gaaatgagcg attgccttca tatccgatga ggaaaaaggt gccgtttttc
     1501 ttgattggtc caccgaggat gccgccgaat tgattcctgc ggaggggatt cttcttggcg
     1561 gtaggcgaga ggaaatagcc gcgcgcatcc agggcatcgt tgcggaggaa gtcgtaggcg
     1621 acaccgtgga aatcgttggt tccactcttc agttgaacgc tgacgaccgc accggagttg
     1681 ttgccgtact cggccgcata atttgatgtg ttgacgcgaa attcctggat cgcatcgatc
     1741 gaaggaagca ggaaaacgga gtttgcgcga ttatcgacga tagagccgcc gtcaagcgta
     1801 acctcctcat gttcgctatc gatgccgttg gcggagaaac tggtgttctg gctgctggcg
     1861 gaagcctgaa cgccgggcgt cagaacagcg agttgtccga agttgcgacc gttgagcggc
     1921 aagcccacgg tctcctgcgg attgacgact ccgctgagcg caccgctggt ggtattcaga
     1981 agcggcgccg cggccgtaac ctcgacgctc tgtgttgtct ggccaatcgc aagcggaata
     2041 tcaacttccg cctgttgtga aagctcgagg gtgatgttcg agcggacggc gcggctaaag
     2101 ccttgcgctt gggcgctgac ggtatacgcg ccgggcagaa gatatcccac acgatatgcg
     2161 ccattggcac cggtggtgac ggtatgagaa acaccggttg aaggcgatgt aacggtaact
     2221 tgcgcgccgg gaatagcggc gccgctcggg tcggtgatcg tcccgaggat tacggtgtcg
     2281 acggtctgcg cagataaagg cgtgacgata acgccgattg cacaggcgac agcgagagcg
     2341 caaatcgcgt acgaaatagc taaattcgcg aaatgacgca gccgagtaca cgtgtttttc
     2401 attctagaac ctcccctttt tctgtggagc gcagcatcag gtacaacgag gacacacata
     2461 tgaagaagct cgccccatat ccaccactta gctaacagac aggcttttat accgtgaaat
     2521 catcgtttca cagttgccag gtagggcctt tgtgtactac ggaatttgcg acgagtcaat
     2581 atgaaacagc cgcgcgaagc gtccgtatcg gtttgattgc tgtgcctgtt agaagagaaa
     2641 ttttacgctg atgccgcttc tgccgtcgcc gcgatcgtcg attcgaacta cggagccgat
     2701 gtattttcgc tgcgaggtca cgttgtgtcc gtggggaaag gtgacttcca gtttcattcc
     2761 aacgaagtag tgcagcatca atgtgatgaa gtgaagtcca tcgcgattga agtcaactac
     2821 cagcgggata tcttcgaggt ctctcagccg cgggtcgaaa ggacgcaaat gcgcggcgag
     2881 cgatagcttc agccgttttc tctttctcaa gtcgcggccg gaatgaggcc gtcggccgtg
     2941 acggtctttt gcggtacaga tttcgtccat ggccatagtg tgatcgcgaa tatggcggcg
     3001 cgctatggga cgccggtact atcgtgctaa acagacgtag tgggaaaagc cgggtgacgc
     3061 tgctaaactt ccgggcggca attgagggcg tcgatttcgc cgacatttcg gcattccggg
     3121 aggaacacat gagttcatcg cgtggcttgc tcggttgtgc tctgttgatt gcgtttagcg
     3181 ttgctccggc atgtgctcag gcgcccggtg gacaagcgcc agcgcgcggg cgcggccctg
     3241 cgggaccggg gttgacgctc acttcaccgg attttcctga cggcggcgtg attccggcga
     3301 aattcacgca ggccgatccg aaacctgttt caccggaact ggattggacg aacgttccgc
     3361 aagggacggt gagtttcgtg ctgctgctgc acgatccgga tacggctttg cagcgcaaga
     3421 gcgacgacgt gctgcattgg ctcgtattca acattccagg cactgcgcac cagcttgccc
     3481 aaggccagcc cgcggatgcg caattgcccg acggcacaat tcaagccaag aatctgcgcg
     3541 gaggcgtcgg attcatggga cccggcgcac cggcggccgg gccggatcat cactacacgt
     3601 ttgaattgtt tgctctggac gaaaagctgg acctcggccc cgatgccacg cgcgcgcaag
     3661 tgatggatgc gattcaaggg cacattctgg ggaagggcgt tttggtggga cgtttccatc
     3721 gaccacagtg aggcg
//
LOCUS       DATNDT010000443         4588 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_144170, whole genome shotgun sequence.
ACCESSION   DATNDT010000443 DATNDT010000000
VERSION     DATNDT010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4588)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4588)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4588
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144170"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1280)
                     /locus_tag="VN788_14480"
     CDS             complement(<1..1280)
                     /locus_tag="VN788_14480"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU21786.1"
                     /translation="MRRSLYTTALVIAALAACVHYSVIVRAQAPSPASAQAQSQAPPA
                     PAAGANLAAGTETGIGTFETRCMGCHGNPNVPQAPAPSAIRQMPPERIYEALTTGLMK
                     PQGDALTDDQKKMLATFLSGRPLGSLKEGDAKDMPNHCASNPPLEDPSSGPEWNGWSP
                     DIVNSRFQNSKDAGLTADQVPQLKLKWAFGYPTGLSAYGQPTIVSGRVFVGTDIGYVY
                     SLDASTGCIYWSYQAKGNVRGAVSIAPVKGHGATKYAAYFGDAHANVYAVDAQTGAEL
                     WLTKVDDHFVARVTAAPKVYKGRVYVPISSSEEFSSANLDYSCCTGRGSVVALDANTG
                     AQVWKTYVVSTPEPVRKNSKGVQQYAPSGGSIWNSPTIDTKRSAIYVGTGDGQTEPTP
                     DTTDAIMAMDLKTGKVLWFYQAQSGDAFMGGCNGP"
     gene            complement(1551..2543)
                     /locus_tag="VN788_14485"
     CDS             complement(1551..2543)
                     /locus_tag="VN788_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21787.1"
                     /translation="MRAKIVAVCALCCLAAIILTGYLSKPVIATGPIATDCDRACLEG
                     LANQYLNALVAHDPKMLPLAADVKYTEQDQVLDVGDGFWGTATAVGHFRLCVSDPVVE
                     QIGCIVTMHEGEHLLIMGLRLRVELGRITEIETNYYRQGGGGPSGIAALDASTPEPIW
                     TETVPPEQRVSRAQMIATANMYFAGLENNDGKGNYPFADDCNRTENGMQTTNNPKLSP
                     NAPFNAFGMSCMAQFKSGYYAIVTRIHHRRYPIVDQERGIVWANVVFDQNGTVQTIHL
                     TNGQTVSMKNFNHPDSLEVTEAFKITGGLIHRIEMVGSGLQYHLNSMWGGISDK"
     gene            complement(2577..3611)
                     /locus_tag="VN788_14490"
     CDS             complement(2577..3611)
                     /locus_tag="VN788_14490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21788.1"
                     /translation="MKNGFLAAMLLTLGGLALGARAGAALQQAPSPAPEWSPSLVKSC
                     DRACLVGFMDGYMNAIFKHDPSAVPTLARDVRMTENTAEMNIGEGMLWRSKVEPTSFK
                     IVVADPVFGQVAEQARVKIGGQDALVAIRLKIDRNQIEEIEQIWTRGVDKTAIPLLTT
                     PRPGLVDDVPASEKTSRDVLIWAANSYFDALEGDDGKIGAFADDCVRHENGYQTVNNP
                     APGGRMMPSPILPNPNTPQGKEQLKISMMSCSAQISTKILNFIKKIRPRRVLVVDEQK
                     GVVATFPFFVEDGTRRGGDPNAPPGMMLNLYTMETFGIRGGLIHEVEAFPFVTIPYGL
                     NNGWTEGAGH"
     gene            3864..4361
                     /locus_tag="VN788_14495"
     CDS             3864..4361
                     /locus_tag="VN788_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21789.1"
                     /translation="MKLPKWVAGAAALVVSLFLGVTLANAQNQDDRDHHDHDQGYHDR
                     DHHDRDHDRDNNYRDHDRDRHEHDRFDDHDRDGARDWYHHHHDAFRVREGRYWHREWG
                     PEIHEGFVFTPDMRRAIRPVPHDLLIRLGPPPPGYRYVLIGDRVCLIDQGYRVHDVLN
                     FELNF"
BASE COUNT          879 a         1376 c         1299 g         1034 t
ORIGIN      
        1 ggcccgttgc agccgcccat gaacgcgtca ccggactgcg cctggtaaaa ccacagaact
       61 ttcccggtct tcagatccat tgccattatt gcgtcggtcg tatctggagt cggctcggtc
      121 tgcccatctc ccgttcccac atagatggcg ctgcgcttgg tatcgatcgt gggcgaattc
      181 cagattgagc cgccggacgg cgcatactgc tgcacgccct tcgagttttt ccgcaccggc
      241 tccggcgtgc tcaccacgta cgtcttccag acctgcgcgc ccgtattcgc gtcaagcgca
      301 accacgcttc cgcggccagt gcagcacgaa taatccagat tagccgacga aaactcctcg
      361 gaagaggata tcggaacgta cacgcgtcct ttatagacct taggcgccgc agtgacgcgc
      421 gcgacgaaat gatcgtccac tttcgtcagc cacagctccg ctccggtttg tgcatccacg
      481 gcgtagacgt tcgcgtgcgc gtctccaaaa taggcggcat acttcgtcgc gccgtgccct
      541 ttcaccggcg cgatgctgac ggcgccgcgc acgttgccct tggcttgata cgaccagtag
      601 atgcatcccg tgctcgcgtc cagtgaatag acgtagccga tatcggttcc cacaaaaacg
      661 cgcccggaga caatcgtcgg ctgaccgtac gcggaaagcc cggtcggata gccgaatgcc
      721 cacttcaatt tcaattgtgg aacttgatcg gcagtcaggc ccgcgtcctt gctattctgg
      781 aatcgcgagt tgacgatgtc cgggctccat ccattccatt ctggccccga agagggatcc
      841 tccagcggag gattcgacgc gcaatggttg ggcatatcct ttgcatcgcc ttctttcaag
      901 ctccccaggg gtcggccgct cagaaacgtg gccagcattt ttttctggtc atccgtcaat
      961 gcgtcgccct gcggtttcat caggccggtg gtcagcgcct cgtaaattct ttcaggaggc
     1021 atctgccgaa tggccgacgg cgcaggcgct tgcgggacgt tgggattccc gtggcagccc
     1081 atgcagcgcg tttcgaatgt gccgatgccg gtttccgtcc cagccgcgag gtttgcgccg
     1141 gctgcgggag cgggcggtgc ctggctctga gcctgcgcgc tcgctggcga gggcgcttgc
     1201 gcccgcacaa tcacactgta atggacacat gcagcgagcg ccgctatcac aagtgccgtg
     1261 gtgtatagag atcgtctcat ttagaaagga ccttttctcg cgagggccgt tttatcgctg
     1321 accactctca cgcgtaatga cgactatctg gtgttctcgc cgccgaatca cgggaacgga
     1381 acagggcggc gctatcttaa tcccgtgtca ttccgagcgg agtatcccgc gcttttgttt
     1441 ttgcgcgaag cgccgggaca cgcagtcgag gaatctctct tggaactgaa atgctgcccc
     1501 gtttgacatc gaaagagaga gattctccgt caagcacgtg tgacctgctg ttatttatcg
     1561 ctgatcccgc cccacatgga attcaaatga tactgcaagc ccgaccccac catttcgatt
     1621 cggtgaatca agccgccggt gatcttgaac gcctcagtga cctcaaggct gtcaggatgg
     1681 ttgaaatttt tcatggaaac agtctggcca ttggtgaggt gaatcgtctg cacggtgccg
     1741 ttctggtcga agaccacgtt ggcccacacg ataccgcgct cctgatcgac gatgggatag
     1801 cgccggtgat gaatgcgcgt gacgatcgcg taatagccgc tcttgaattg tgccatacag
     1861 ctcatgccga aggcattgaa cggcgcattc ggcgaaagct ttggattatt ggtggtctgc
     1921 atgccgttct cggtgcgatt gcaatcgtcg gcgaacgggt agttcccttt tccgtcattg
     1981 ttttcgaggc ctgcgaaata catgttggcc gtagcgatca tctgcgcacg cgagacgcgc
     2041 tgctcgggcg gaacagtttc ggtccaaatc ggttcggggg tgctggcgtc aagcgcggcg
     2101 atgcccgacg ggcctccgcc gccctggcga tagtaatttg tttcgatttc ggtgatgcgt
     2161 ccgagctcga cgcgcaggcg caagcccatg atcagcagat gttcgccctc gtgcatggtc
     2221 acgatgcagc cgatctgctc gaccacggga tcggaaacgc acagccggaa atgcccgacc
     2281 gcagtcgctg tgccccagaa accgtcgccg acgtccagca cctgatcttg ttcggtgtac
     2341 ttcacgtccg ccgcaagcgg cagcatcttg ggatcgtgcg ccactagagc gttcagatat
     2401 tgattcgcca atccttccag gcaagcacga tcgcaatcag ttgctatcgg gcctgtggcg
     2461 atcaccggtt tgctcaaata tcccgtcagt atgatcgctg ccaggcaaca caaagcacac
     2521 accgctacga tcttcgcacg catcgccatc ctccttcgaa ttctctccga ccgggatcaa
     2581 tggcccgcac cttcggtcca gccattattt aggccgtaag gaatggtgac gaatggaaac
     2641 gcctcgactt cgtggatcag cccgccgcgg attccgaaag tttccatggt atagaggttc
     2701 agcatcatcc ccggaggcgc attcggatcg ccgccgcgtc gcgtgccgtc ttcaacgaaa
     2761 aacgggaatg tggccaccac gcccttctgc tcatcgacaa ccagcaccct gcgggggcga
     2821 attttcttga tgaagttgag tatcttcgtg gagatttgcg cggagcagct catcatgctg
     2881 atcttcaact gctcctttcc ctggggcgta ttcggattcg gaagaatcgg cgacggcatc
     2941 atgcggccgc cgggagccgg attgttgacg gtctgatagc cattctcgtg acgcacgcag
     3001 tcatcggcga aggcgccaat tttgccatcg tcgccttcga gtgcgtcgaa gtaggagttc
     3061 gcagcccaaa tcaatacgtc gcgcgaagtt ttttccgaag caggaacgtc gtccacgagc
     3121 cccggtcgcg gcgtagtgag cagcgggatc gccgtcttgt ccacgccgcg ggtccagatt
     3181 tgctcgatct cctcgatttg gttgcggtcg attttcaggc ggatggccac gagcgcatcc
     3241 tgcccgccga ttttcacgcg cgcctgctcg gcaacctgac cgaaaaccgg gtcggcgacg
     3301 acaatcttga agctggtcgg ctcgaccttc gagcgccaga gcattccctc gccgatattc
     3361 atttcggccg tattttccgt catgcggacg tcgcgcgcga gagttggtac agcagaggga
     3421 tcgtgcttaa agatggcgtt catatagcca tccatgaaac caacgaggca ggcgcggtcg
     3481 caagacttga cgagcgatgg agaccattcc ggtgccggcg acggtgcttg ctgcagcgca
     3541 gcgcctgcgc gagcgccgag cgccagcccg cctagcgtca atagcatcgc cgcaagaaat
     3601 ccgtttttca ttgagttagc tcgcccgcgg cattgcgcct ttctacatcc aaacgggacg
     3661 cacaataccg cgctctcgct cgccggtcaa tggcgtgcgt aacttcccgg cgacaccgcg
     3721 attggacatt cgtacatggc gatttgcggt tagctagtgg attctgagta tggtggactg
     3781 aacgccatgt tttacaattt taggaactcc acccggccca attggtgcga ggttcgaatc
     3841 gtggactttc acaaggaggt ttcatgaagc tgccaaaatg ggttgccggc gccgcggctc
     3901 tggttgtcag cctgttctta ggggttacgc tggccaacgc acagaatcaa gatgatcgcg
     3961 atcatcatga tcacgatcag ggatatcacg accgtgacca tcacgaccgg gaccacgatc
     4021 gcgacaataa ctatcgcgat cacgaccgcg atcgccatga gcacgatcgg ttcgacgacc
     4081 acgatcgcga cggtgcgcgc gattggtatc accatcatca cgacgctttc cgagtgcgcg
     4141 agggtcgcta ttggcaccgc gaatggggtc cggagattca cgagggattt gtttttacgc
     4201 cggacatgcg gcgagccatt cggcctgtcc cgcatgattt actcattcgg cttggcccgc
     4261 cgcctccggg ataccgctac gttctaatcg gcgatcgagt ttgcttaatt gaccaggggt
     4321 atcgggtcca cgacgtgctt aacttcgagt tgaatttcta aatttcaagg gccaccgctt
     4381 gcttctaaca gcggaaccgg cgccccgcac cgccggttcc gctttttttt ccttcccgcc
     4441 agttcctagg ccggtttctt tcttgcctct ttgcctcttc cacagcgcga cggtgtgggc
     4501 accggctcca gtttgtgcta cgcttttgcg ggacgagaag ttcgcggccg cgcattagtt
     4561 tggaaaacgg ctccggcatc tcgctttc
//
LOCUS       DATNDT010000444         4300 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4104997, whole genome shotgun sequence.
ACCESSION   DATNDT010000444 DATNDT010000000
VERSION     DATNDT010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4300
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4104997"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1246
                     /locus_tag="VN788_14500"
     CDS             <1..1246
                     /locus_tag="VN788_14500"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU21790.1"
                     /translation="DQVVNGALRMLDVVARPPELQSLDAHGTQMPAAYSSYLQGRGYL
                     QNYDKPENLDSAIGAFERALALDPNYALAYAGLGQARWEKYQASHDPKSVDQARQACQ
                     RALNLSEQLAAAHVCMGQLESGTGEYEKAVKEFERALTIEPTNDDAYRSLADAYESLG
                     QPAKAEETCQRAIDLRPQYWAGYNWLGTFYYRDARYADAASMFSQVVALAPDSFRGYS
                     NLGAVYYYQGRYTDAIAMLQRSIAIRPSADAFSNLGSAQFYLRHYDDAVTAYEKAVQL
                     SQNNYILWWNLADGYYWDSAKRAQAGGAYRKTISLAHEALRINPRDAAALGVVAYCHA
                     MLGERKPALDYLQQGLKLAPQDSEMRFKAALIYNQFGDTAQTFAWLSKALAGGFSATI
                     VRDTPNFDLLRSNPRFEALFKT"
     gene            1270..1599
                     /locus_tag="VN788_14505"
     CDS             1270..1599
                     /locus_tag="VN788_14505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21791.1"
                     /translation="MNHTATGRAQRKTVTIHVNKDGEVQHAEPEYFELSKSNQEEIFW
                     QASDGKTYFTVEFEKESPFYESQFSSEFPASGLVRRDVLPDLQKRYKYTVRAGGAELD
                     PGGVITK"
     gene            complement(1646..2182)
                     /locus_tag="VN788_14510"
     CDS             complement(1646..2182)
                     /locus_tag="VN788_14510"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU21792.1"
                     /translation="MKRWVWLVVIGLAVPLTARAQSTTSDPISTQLRQVLDRFATNLV
                     AAAEEFPVDKYTYHPTPEGMTVGHTMEHIAQVNDFACSTVGGVAAPQYTKVGETDKDK
                     LVEGLKASMDFCRQQFAGLTDAKLADSVPGFGGRSSTRFAAAMEVTNDLIDHYATLSV
                     YLRINGLLPPSAQKRRKP"
     gene            2411..2869
                     /locus_tag="VN788_14515"
     CDS             2411..2869
                     /locus_tag="VN788_14515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21793.1"
                     /translation="MGRFVALYRMSSCAVDARRTLLYVAIALLIVVLAHRRVQVISAP
                     GRAMPVAEVAGKNWRYDLSFPVRNLSTVEELIQCESQGQNISRLDSNGQVSRGILQFN
                     GTSTWNEMERRFDFYGDPRNPTAAIHMADMMISSGLVGRWTCARSLGLTK"
     gene            complement(2967..3341)
                     /locus_tag="VN788_14520"
     CDS             complement(2967..3341)
                     /locus_tag="VN788_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21794.1"
                     /translation="MPNGNGRRVRRSARVTLRIAMRIYEHGTNKRFALEEAHSMKVSL
                     WGGLVALKSAVSAGQKLVVVNRATSETREARVVSVGPLQLNKRLVGFEFLEPSPKFWG
                     VVFPPAGAQPMPARSVYRSRVS"
     gene            3595..4155
                     /locus_tag="VN788_14525"
     CDS             3595..4155
                     /locus_tag="VN788_14525"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU21795.1"
                     /translation="MKVRWISTAGIVTLLILATAAPPLRAQTTSPKPGEEVLASWNYI
                     GHKLITMAEDFPESKYEFKPAPASRTFAAQLLHVAGTNYAYVNAFSGKKMGPEDNDPS
                     RSVYKTKAQVVAVLRKSFTDGATLIKEHADQLNRIITDPDSGRPSSELLSWLNDCEHS
                     GEHYGQLVVYYRVNGLVPPDSRPRPK"
BASE COUNT          925 a         1313 c         1155 g          907 t
ORIGIN      
        1 ggatcaagtc gtgaacggag cgctccgaat gctcgatgtc gtcgcgcgcc cgcccgaact
       61 gcaatcgctc gacgcgcatg gcacccagat gcctgccgct tacagttcct atctgcaagg
      121 ccgcggttat ctgcagaatt atgacaaacc tgaaaatctc gatagcgcaa tcggcgcctt
      181 cgagcgggcg ctcgcattgg atcccaacta tgctctcgcc tacgccggac tcggccaagc
      241 gcgctgggaa aaatatcaag cgagccacga tccgaaatca gttgaccagg cgcgccaggc
      301 ttgccaacgc gcattaaatc tcagtgaaca acttgcggcc gcgcacgtct gcatgggcca
      361 actcgaaagt ggcaccggag agtacgagaa agctgtgaag gaattcgaac gcgcgctcac
      421 catcgagcct accaatgacg atgcctaccg cagcttggcc gacgcctacg aaagcctcgg
      481 ccagcccgcc aaggcggaag aaacctgcca acgcgcgatt gatttgcgtc ctcaatattg
      541 ggcaggctac aactggctgg gaacgtttta ctatcgcgat gcccgctacg ccgacgcggc
      601 ttccatgttt tcgcaagtgg tcgcgctggc tcccgatagc ttccgcggat actccaatct
      661 cggcgccgtt tactactacc agggccgcta cactgacgcg atcgccatgc tgcagcgttc
      721 gattgccatc cggccgtcgg ccgacgcctt cagcaatctc ggcagcgccc agttttatct
      781 ccgccactat gacgacgctg taactgcata cgaaaaagcc gtgcagctca gccagaacaa
      841 ttacattctc tggtggaacc tcgccgatgg gtattactgg gattccgcca aacgcgcgca
      901 agccggcggg gcctatagaa aaacgatttc tctcgcgcac gaagccctgc gcattaatcc
      961 gcgcgacgcc gccgccctcg gcgtcgttgc ctactgtcac gccatgctgg gagagagaaa
     1021 gccggcactc gactatctac aacaaggcct gaagctggcc ccgcaagatt ccgaaatgcg
     1081 tttcaaggca gctttgattt acaaccagtt cggcgacacc gcgcaaacct tcgcttggtt
     1141 gagcaaagcg ctcgctggtg gcttttccgc tacgattgtg cgcgatacgc cgaacttcga
     1201 tctcctccgg tccaatcctc ggttcgaagc gttgttcaaa acctaatgtt ctctcgaaaa
     1261 ggagctgcga tgaaccacac ggcgacaggt cgagcgcaaa gaaaaacggt gacgattcac
     1321 gtcaataaag atggagaagt ccagcatgcc gaacccgagt atttcgaact ttccaaaagt
     1381 aatcaggaag agattttctg gcaggccagc gacgggaaaa cctactttac agtcgagttc
     1441 gagaaggaat cacctttcta cgagtcgcag ttcagcagcg aatttcccgc ttcgggcctc
     1501 gtgcgccgcg acgtccttcc cgatctgcag aagagataca agtacacagt gagggccggt
     1561 ggcgcggagc tcgatccagg cggcgtcatc acaaaatagc gtcaagtcct gcgcgtatcc
     1621 cgcgcgtcgg tcttacatcg aatccctacg gtttccgcct tttctgcgcg gaaggcggca
     1681 gcaggccgtt gattcgcagg taaacggaaa gcgtagcgta gtgatcgatc aggtcattgg
     1741 tcacttccat tgccgcggca aatcgcgtcg acgaccgccc gccgaaaccc ggaacggaat
     1801 ctgcgagttt cgcgtccgtc aaccccgcaa actgctgtct gcaaaaatcc atcgaggctt
     1861 tcagtccttc taccagcttg tccttgtccg tctcgccgac cttcgtatat tgcggggcgg
     1921 ccaccccacc gacagtcgag cacgcgaagt cgttgacctg cgcaatgtgc tccattgtgt
     1981 ggcccactgt cattccttcg ggagtcggat ggtaggtgta cttatccact ggaaattctt
     2041 cggcggcggc caccaggttc gtcgcaaacc gatcgagaac ttgccgcaac tgcgtggata
     2101 tgggatcgct cgtcgtactt tgcgcccgcg cggtcagcgg cacggccaaa ccgatcacca
     2161 ccaaccaaac ccatcgcttc atgttttttc tcctcgaaat taattttgcg aatcaactct
     2221 tcagccgaat tctaaatttg agcttctgct ccgcgcatcg catcgtaacc cttcttctgt
     2281 ccgcgacaca acgaccttgc cttatgcggc ctgtccttat tgccagttaa ccgtcacgtg
     2341 aaagccgcga cggaacgatc ctcctccggg atattcttaa actgggggac ggcacgtgcg
     2401 ggaaaaatac ttgggcaggt ttgtggcgct gtatcggatg tcgtcatgcg cggttgatgc
     2461 gcgcagaacg ttgctctatg ttgcgatcgc tttgttgatt gttgtgctgg cccaccgccg
     2521 cgtgcaggtt atttcagcac ctggccgggc gatgcctgtg gcggaagtcg ccggcaaaaa
     2581 ttggcgctac gacctcagtt tccctgtgcg caacctctcg actgtcgaag agctcattca
     2641 gtgtgaatct caaggccaaa atatttcgcg cttggattcc aacgggcagg taagccgcgg
     2701 aatacttcag ttcaatggca cttccacgtg gaatgaaatg gaacggcgct tcgattttta
     2761 cggtgatccg cgaaacccta ccgcagcgat ccacatggcc gatatgatga tttcgagcgg
     2821 cctcgttgga cgctggacct gtgctcgctc tctcggtctc acaaaatagc ggaattcgtc
     2881 gtcctgtggg catcgcgtcc cgcttctgcc cgctcttcga aagaaagaag tgaaccgtcg
     2941 gcgtccgacg atgaattgct atgcgctcag cttacccgcg agcggtaaac cgatctcgcc
     3001 ggcattggct gcgcgccggc gggcggaaac accacgcccc aaaatttggg tgaaggttcc
     3061 aaaaactcaa agcctaccag ccttttgttc agctgcaggg gccccacgga aaccacccga
     3121 gcttccctgg tttcgcttgt cgctcgatta accaccacca gcttttggcc tgcgctgacg
     3181 gcggatttca gcgccacgag cccgccccac aggctcactt tcatggaatg ggcctcttcg
     3241 agggcgaatc gcttgtttgt gccgtgttcg tagattcgca tcgctatgcg taaagttaca
     3301 cgggcgctgc gccggactcg cctgccgttc ccgttgggca tgcctgcctc cgtcgtataa
     3361 attggcacgg tggatcgcta ggatggacga aaaatcgaag ctatccaatg gtaccaaagg
     3421 actaagggat tccccgaggc ggccgcatcc atccgccccg cgatcgtgag accgccaggc
     3481 ttgaccgccc cgaaaggatg gatgaacatc tctccggctc cgtgcactcc tgaccgattt
     3541 ggtcgtgccc cggagaaaac gcgcggcctc tatagccaat aggaggaaag agtcatgaag
     3601 gtacgatgga tatccacggc cggaattgtc actctgctga ttctcgcaac cgcggcgccg
     3661 ccgcttcgcg cccagaccac gtctccaaaa cccggcgagg aagtgttggc gtcgtggaat
     3721 tacatcggcc acaagctgat caccatggcc gaagactttc ccgaaagtaa gtacgagttc
     3781 aagcccgcgc ccgcatcgcg cacctttgcg gcgcagttgc ttcatgttgc gggcacgaac
     3841 tatgcgtacg tcaatgcgtt ctccggaaag aaaatgggtc cggaagataa tgatccgtcg
     3901 cgcagcgttt acaaaacgaa agcccaggtt gtcgccgtgc tgcggaaatc cttcaccgac
     3961 ggcgccacgc tgattaaaga acacgcggat cagttgaacc gcatcatcac cgaccccgat
     4021 tccggccggc catccagcga attgctctca tggctgaacg actgcgagca ctccggcgaa
     4081 cattatggcc agctcgtagt ttactatcgc gtgaatggcc tcgttccccc ggactcgcgc
     4141 ccgcgcccga aataggcctg cgtcctgccg gagatatgga ggcggtgttc cccgacgggc
     4201 acggttgccg ccggaggtac gaagagcgcc gctctacgaa ctcacttcag ccccgccgtt
     4261 ttcatcgttc cctaacacgc gtttggatat tttctggcat
//
LOCUS       DATNDT010000445         7408 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1254930, whole genome shotgun sequence.
ACCESSION   DATNDT010000445 DATNDT010000000
VERSION     DATNDT010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7408
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1254930"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="VN788_14530"
     CDS             complement(<1..545)
                     /locus_tag="VN788_14530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HXU21796.1"
                     /translation="MKNTAVFGIFPDSVTVGEAVEALKQAGFRNTDISVLFPENTGTK
                     DFGHERHTKAPEVAVAGASSGAIIGGALGWLAGIGVLAIPGIGPFIAAGPIMAMLSGI
                     GVGGTIGGLTGSLIGLGIPEYEAKRYEGRIRNGGILLSVHCDSSEWVKRARTALEDTG
                     GSDIATSGEAKADAAAASSSDR"
     gene            complement(664..1335)
                     /locus_tag="VN788_14535"
     CDS             complement(664..1335)
                     /locus_tag="VN788_14535"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HXU21797.1"
                     /translation="MPQLTRVRLNGACRLIPSLYPASGILDTISTPEDLPFIFELESW
                     TNDRISTELGILHRLPPDEWVVGRPMASVVMAAFCHPRPGGGRFNGPDRGAWYAGTDL
                     DTAHAEVVYHRTRELAEIGIFETRLQMRLYLADFRAVFHDIRAREPEHQPLHDPASYT
                     ASQEFARALLASGSNGIFYRSVRKPGGECIACFRPALVVNVREAGHFEYLWPGAREPQ
                     IQRLA"
     gene            complement(1336..1785)
                     /locus_tag="VN788_14540"
     CDS             complement(1336..1785)
                     /locus_tag="VN788_14540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007582627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbcA/ParS/Xre antitoxin family protein"
                     /protein_id="HXU21798.1"
                     /translation="MELPARRAKAIPQIASIPPERRRDPKIRRQMSGPAIRAFFNIAE
                     VWQLSGEDQQALLGWPAESTFYKYKAGQVGTLSYDMLMRISLILGIFKDLRILYPERD
                     LADRWVHLPNSNPLFGGAPALALMTGSGLDGLYQVRRLLDARRGGWS"
     gene            1951..2295
                     /locus_tag="VN788_14545"
     CDS             1951..2295
                     /locus_tag="VN788_14545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21799.1"
                     /translation="MQLALILPAALFMAAVLVGVGDAPQYDLARVAQRIVMWYSARMW
                     TLWLLLLGLPFAVLVTGCATLLRRWNHDVELPHAARLAMIPAPLATLSVAGTTLLSAG
                     ILAIVVLHMLAN"
     gene            complement(2411..2839)
                     /locus_tag="VN788_14550"
     CDS             complement(2411..2839)
                     /locus_tag="VN788_14550"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU21800.1"
                     /translation="MAGDGEPRKVHILLIEDNPGDVALIRMALRNAGLDCDLTVIDEG
                     GEAMAFVQQQGKYGASRAPDLAILDLNLPKNDGLEILAAMRGNQAFVEVPVAVLSSSS
                     WPRDRTKLEVFNIARYITKPPDLDEFMRIGLILKELLAAG"
     gene            complement(2841..5093)
                     /locus_tag="VN788_14555"
     CDS             complement(2841..5093)
                     /locus_tag="VN788_14555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU21801.1"
                     /translation="MLSFRDIPVKQKLMMATMVTTAVALLLAGIGIVLADSLLFRGYL
                     QRDLSGLAHIIADNSTAALAFNDQRSAAETLAALRVRTHLVAACIYRRDGTMLADYRR
                     TGAQSGCPAPGAPDGIRFTSAALTVSYPILLSGHRIGTLVLLYDLGEIQERMKLYGLT
                     VLGVLLASILIAFVLSSRLRTIIAAPISQLARAATSVARTKDYSIRARKNSGDELGVL
                     VDAFNEMLAGIQSRDDNLRNALLDRQEALKEAQNARESLETTVASIGDAVIATDIEGR
                     IVLANRVAQSLMKLLESEMIGKHLDEVFRIFNEFTGAKVESPVTKVLREGSIVGMANH
                     TVLIARDGTEIPIDDSGALIRGTSGEIQGTVLVFRDATAKRRADETGRLLASIVESSD
                     DAIIGHGLGGVIIAWNRGAERIFGYSAEEMIGKPASALTAPGHVDEIPEILERIARGE
                     RVDQYQSPRRTKSGTVIDVSITISPMYDALGRIVGASKIARDVTGQVRAAERLAQMNA
                     DLQRANENLARSNDDLERFAFVASHDLQEPLRMITVYSQLLIRTHSGEFDDQAAMCVD
                     NIVGGTKRMRELLSDLLAYAEIGGMPEEPVQTVDLNVVIEKVTQNLKVAIEDSGAVIT
                     AGRLPSLSAYEGHLIPLFQNLIGNAIKYRGEQPPRIHVSAQMGDGELRFSVADNGIGI
                     PPQHHQKIFTPFKRLHGKATPGTGIGLAICQRVVERYGGRIWVESDAGKGATFIFTVP
                     DIAIHAVEEN"
     gene            complement(5117..5557)
                     /locus_tag="VN788_14560"
     CDS             complement(5117..5557)
                     /locus_tag="VN788_14560"
                     /inference="COORDINATES: protein motif:HMM:NF025069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HXU21802.1"
                     /translation="MKAAFLLNFTKFVEWPSSAFATPDSPLAICILGGDPFGGALDQI
                     VEGEVVNGRKLVIKRLTHAPPPKTCQVLFAAKSEKDIANILAAVGPGVLTVGEGESFL
                     RDGGIIAFVIEDRRVRFDIKETAAAHAGLKLSSKLMNVARFVEK"
     gene            complement(5701..>7408)
                     /locus_tag="VN788_14565"
     CDS             complement(5701..>7408)
                     /locus_tag="VN788_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU21803.1"
                     /translation="RGFGTRLTRSVLVLIDGRTVYTPLFDGTYWEVQDTMMEDIDRIE
                     VIRGPGGTIWGPNAVNGVINIITRDSKDTRGFLASAGGGNEEQAFGSFRYGGGNGRGF
                     NYRFYGKGFTRSPEVHPDHRNFDDWRSAQAGFRMDWEKNTRDTFTLQGDLYDEVAGES
                     VQATSYTQPYSQIVDANALLSGGNIMGRWKRTLGEGSDIQVQAYYDRTNRHEPNLGEL
                     RDTFDLDFVHRFRLPARQQISWGLGGRVSPVNDIEVVSGLTFVPSKRTDYLLTGFFQD
                     EIGLVDRRLSLTLGTKVLRTNFTGVALEPSAQLLWTPTDKQTVWAAFTHAVRTPSDVE
                     EDFYLTGYITTTADGTPYFARFNANPNFAPEQLNGYELGYRRVLGKTIYVDVAGFYNH
                     YHNLFSEDIIGQPFLESDPPPEHLLLPAQFRNGLLGYTKGVEIAPEWRPTNFWRLRGS
                     YSYLHMNLGRSPHSGDVGTAPMIVGSSPQHQLMIQSSFDLSKKLQLDLDYRYIGALPG
                     QIVPAYSTADARLGWRFSPEFELSFVGRNLLQPSHAEYGSDPGGLVAIRRSAYVKLTW
                     SR"
BASE COUNT         1527 a         2209 c         2144 g         1528 t
ORIGIN      
        1 cgatccgacg atgatgcagc ggcggcgtcc gccttcgcct cgccgctagt ggcgatgtcg
       61 cttccgccag tgtcttcgag cgcggtccgg gcgcgcttca cccactcgga agaatcgcaa
      121 tggaccgata acagaatgcc cccgttgcga atccgtcctt cataccgttt ggcttcatac
      181 tctggaattc caagcccgat cagcgatccg gtcaggccgc cgatggtacc gcccacgccg
      241 atgccgctga gcatggccat gatcggaccg gcggcgataa aggggccgat ccctggaata
      301 gcgagcacgc caatgccggc aagccatccc aaggcgcctc caataatagc tccggagctc
      361 gcgcccgcca cagctacctc cggcgccttt gtatgtcttt cgtgaccaaa atccttcgta
      421 cctgtgttct ccgggaacag gacggagatg tcggtgttac ggaagcctgc ctgcttgagc
      481 gcttccaccg cttcgcctac cgtaacggaa tcaggaaaga tgccaaaaac agcagtattc
      541 ttcatcgctc accttgaaag cactttgcca gccaatcgta cctggttgaa aaatcgggcg
      601 ctccggctat cgaagatgcc gtgcgttacc gaaaacatga aaaatttact ttcattaata
      661 ttatcacgcc aatcgctgga tttgaggttc acgggcgccg ggccatagat attcgaagtg
      721 tccggcctcc ctgacgttta cgaccagcgc aggtcgaaag caggcgatgc attctccgcc
      781 cggttttcgc acgctacggt agaaaatacc attggaaccg gaggccagca acgccctggc
      841 gaactcctgg gaagcggtgt aactggccgg gtcgtgaagc ggctggtgct ccggctcgcg
      901 tgcgcggatg tcgtggaaaa cagcccggaa gtccgcaagg taaagccgca tctgcaaacg
      961 cgtttcgaaa atgccgatct ccgcgagttc ccgcgtgcgg tgatagacaa cctcggcgtg
     1021 ggctgtatcg agatcggtcc ccgcatacca tgcgccgcga tcggggccat tgaagcgacc
     1081 gcctcccggc cggggatggc agaatgccgc catcacaacg ctcgccatgg gccgcccgac
     1141 aacccactcg tcgggaggca ggcgatgcag aatcccgagc tcggtcgaaa tgcggtcgtt
     1201 ggtccagctt tcgagttcga aaatgaaagg caaatcttca ggagtggaga ttgtgtccag
     1261 aatgcccgat gccggataga ggctgggaat caggcggcag gcgccattca gccggacgcg
     1321 cgttaattgt ggcaactaac tccagccgcc ccgccgggca tcaagcagtc ttcgtacctg
     1381 atacagtccg tcgaggccgc tgccggtcat cagcgcgagc gccggcgcgc cgccaaacag
     1441 cgggttactg ttggggagat gcacccagcg gtccgccaga tcccgctcgg ggtacaaaat
     1501 gcgcaaatcc ttgaagatgc ctaagatgag ggaaattcgc atcagcatgt catacgagag
     1561 cgttccgact tgcccggcct tgtatttgta gaaggtcgat tcggcaggcc accctagtaa
     1621 ggcttgctga tcttcgccgc tcagttgcca aacctcggcg atattaaaaa aggcccgaat
     1681 tgccgggccg gacatctgcc tgcgaatctt tggatcgcgg cgccgctcgg gaggaatgga
     1741 ggcgatttgg gggatcgctt tggctcgacg ggctggtaac tccatatctg gagtatacgc
     1801 tccagattga gattagggca agggaccgct gatcggtagt gagtcagttt cagattcggc
     1861 cgacccgccg gtgggcataa gcgccttgcg gcgacttgta ctttgtgata taaagtctgc
     1921 atgcacagcc tcacacgcgt catagcggca atgcaactgg cccttatcct tcctgccgcg
     1981 ctcttcatgg ccgcggtcct cgtgggcgtg ggcgatgcgc cgcaatacga cctggcccgc
     2041 gtcgcgcagc ggatcgtgat gtggtattcg gcgcggatgt ggacgctctg gttgctgctg
     2101 cttggtctgc cgtttgccgt gctcgtcacc ggctgcgcca ccctgctgcg cagatggaac
     2161 catgacgtgg aactgccgca cgccgcgcgg ctcgccatga tcccggctcc cctcgcgacg
     2221 ctctccgtcg ccgggacgac gctcctatcg gcggggatat tagcgattgt ggtgctgcat
     2281 atgctcgcga actgaccgcg tcgccggttg cctcatgcag ggcacctttc ggctgcgggc
     2341 gcgctttcga gtgggtccag ccgactgaaa gccaggctgt gggcaagatt gcccgcccca
     2401 ccagatcgat ttacccggca gccagtaatt ccttcaagat caacccgatt ctcataaatt
     2461 cgtctaaatc gggcggcttg gtgatgtacc gcgcgatatt gaaaacctct aactttgtcc
     2521 ggtcacgcgg ccacgatgaa gaacttaaga cggccacggg cacttccacg aaggcttgat
     2581 tcccgcgcat cgccgccagg atttccagcc catcgttctt gggcagattc aggtcgagga
     2641 tcgcgagatc gggtgcgcga ctggctccgt acttcccttg ttgctgtacg aatgccatcg
     2701 cctcgccgcc ttcgtcaata acggtcaaat cacaatcgag acccgcgtta cggagggcca
     2761 tccgaatgag cgcaacgtct cccggattgt cctcgattaa aagaatgtga acttttcgcg
     2821 gctctccatc tcccgccatt tcaattctcc tcaacggcgt ggatcgcaat gtcgggaacc
     2881 gtaaaaatga aggtcgctcc tttgccggcg tcggattcca cccagatccg gccgccgtac
     2941 cgttccacga cgcgctggca gatcgccaag ccaattccgg tgccaggcgt tgccttgccg
     3001 tgcagccgct tgaatggggt gaatattttt tggtgatgct gaggtggaat gccaattcca
     3061 ttatcggcca ccgagaaccg cagttcgcca tcgcccattt gcgccgagac gtgaattcgc
     3121 ggtggctgct cgccacgata tttgatagcg ttgccgatca ggttctgaaa caaggggata
     3181 agatgcccct cataagcgct cagggagggt agccggccgg cggtaatcac ggcaccacta
     3241 tcctcgatgg caactttcag attctgagtc actttttcga tcaccacgtt caggtccacg
     3301 gtttggaccg gttcctccgg catcccgccg atttccgcgt aagcgagcag atccgacagc
     3361 agctcgcgca tgcgtttcgt gccgcccacg atgttatcca cgcacatggc tgcctggtcg
     3421 tcgaactcgc cggagtgggt cctgataaga agctgcgaat agaccgtgat catccgcaga
     3481 ggctcctgca agtcgtggct ggccacgaaa gcgaagcgct ccagatcgtc gttcgaccgc
     3541 gccagatttt cgttggcccg ctggagatcg gcgttcatct gggccaagcg ttccgccgcg
     3601 cgcacctgcc cggtgacatc gcgagcgatt ttcgatgcgc cgacgattcg ccccagtgca
     3661 tcgtacatcg gagatatcgt gatcgacaca tcaatcacgg taccgctttt cgtccgccgc
     3721 ggcgactgat attgatccac ccgctctcct ctggcaatac gttccagtat ctccgggatc
     3781 tcatccacat ggccgggggc ggtgagcgcg gatgccggct tgccgatcat ctcctccgcc
     3841 gaatagccaa aaatgcgctc ggccccgcga ttccaggcga tgatcactcc tcccaagccg
     3901 tggccgatga tggcatcgtc ggaagattcc acgatcgagg cgaggaggcg gcccgtttca
     3961 tcagcacgcc gcttcgccgt ggcatcccgg aacaccagta ccgtgccctg tatttcgccg
     4021 ctcgtgccgc gaatcagcgc gccactatcg tcgatcggga tttccgttcc gtcccgcgca
     4081 atgagtaccg tgtggttggc cattcccacg atggagccct cccgcaatac cttcgttacc
     4141 gggctctcca ccttggctcc tgtgaattcg ttaaagattc gaaacacctc atcgaggtgc
     4201 ttgccgatca tttccgattc cagcaatttc atcaacgatt gggcgacgcg attggccagg
     4261 acaatgcgac cttcgatatc ggtggcaatg accgcgtccc cgatgctggc gacagtcgtt
     4321 tcaagagatt cccgcgcgtt ctgggcctct ttaagagctt cctgccgatc aagaagagcg
     4381 tttctcaaat tatcgtccct ggactgaatg cccgcgagca tctcattgaa cgcgtctacg
     4441 agaacgccaa gttcatcgcc cgagtttttc cgcgcacgaa tgctatagtc cttagtccgc
     4501 gctacggaag tcgcggcgcg ggcgagttgg gaaatcggcg cggcgatgat ggtgcgcagt
     4561 ctcgatgaga gcacgaaggc aattagaatg gatgccagca gaacgccgag gacggtaagc
     4621 ccataaagct tcatgcgctc ctggatttcg cctaaatcgt agagcagcac caacgtgccg
     4681 atccgatgtc cacttagaag aatggggtat gagacggtta gggccgcgct ggtgaagcgg
     4741 atgccatcgg gcgcaccggg cgccggacat ccggattgcg cgccggtacg tctataatcc
     4801 gcgagcatcg taccgtcgcg ccggtaaatg caggcggcga ccaaatgagt cctcacccgc
     4861 aacgccgcca acgtttcggc agcggaccgc tggtcgttga aggctaatgc agccgtgcta
     4921 ttgtcggcga tgatatgggc cagcccggac aagtcacgct gcagatatcc gcggaagagg
     4981 agggagtcgg cgaggacgat gccgatgccc gccagcagca gggccaccgc ggtggtcacc
     5041 atagtggcca tcatcagttt ctgcttgacc ggtatgtcgc ggaaactaag catggctagc
     5101 cactgcgccc gatactctat ttctcaacaa atcttgccac gttcataagc ttggaactga
     5161 gcttgagtcc agcatgagcc gcggcagttt ctttgatatc gaaccgcaca cggcgatcct
     5221 caatgacgaa agcgatgatg ccgccgtcgc gaaggaagct ctccccttcg ccgacagtca
     5281 gaacgcccgg ccccacggca gccaggatat tggcaatatc tttttccgat ttggcggcaa
     5341 ataggacctg gcacgttttg ggcggcggcg catgggttaa acgcttgata accagctttc
     5401 gcccgttcac aacttcccct tccacgatct ggtccaacgc gccgccaaag ggatcgccgc
     5461 caaggatgca gatggcgagc ggcgaatcag gtgttgcgaa agccgaagag ggccactcga
     5521 cgaacttggt gaaattgagc aaaaacgccg ctttcacttg atactccata ggcgcggccg
     5581 cagctcgcga ggtacgctgc gccgataaac ccaggcccgc cactagacac atcataatcg
     5641 cggggttccg caccactggc cagccaggag gtgcttttcg ggattcgaga ctgttatccc
     5701 tcacctgctc catgtcaatt tcacataggc gcttcttcta atggcaacaa ggccgccggg
     5761 gtcgcttccg tactccgcat gcgagggctg gaggagattt cgaccgacga acgacaattc
     5821 aaactccgga ctgaaccgcc acccgagccg ggcatcggcg gtcgaatagg cggggactat
     5881 ttggcccggc agagcgccta tatacctgta gtcgagatcc aactgaagtt tcttcgacag
     5941 gtcgaaactg gattgaatca tcagctgatg ctggggactg gaaccgacga tcatgggcgc
     6001 ggtacctacg tcgccagagt ggggcgaccg tcccagattc atgtgaaggt aggaatacga
     6061 cccgcgcaac cgccagaagt tcgtgggccg ccactccgga gcgatctcca cccctttcgt
     6121 atagccgagc aatccattgc ggaattgcgc gggaagcaat aaatgctccg gcggcgggtc
     6181 agactcaaga aacggctgcc caattatgtc ctcgctaaag agattgtggt agtggttgta
     6241 gaatcccgcg acatcgacat agattgtttt gccgagaacg cggcgatagc cgagctcgta
     6301 gccattgagt tgttccggcg cgaagtttgg gttggcgttg aaacgcgcga aatagggcgt
     6361 tccatccgct gtcgtcgtga tgtagccggt gagatagaag tcttcctcga catcggaagg
     6421 agttcgcaca gcgtgcgtaa aagccgccca cacggtttgc ttgtcagtgg gcgtccacaa
     6481 gagctgggcg ctcggttcga gggcgacgcc ggtgaagttg gtgcgcaaca ctttcgtgcc
     6541 tagagtcaga gacaggcggc ggtccacgag tccaatctca tcctgaaaga acccggtgag
     6601 cagatagtcc gtacgcttgc tcggaacgaa cgtcagcccg gagaccacct cgatatcgtt
     6661 gaccggactg acgcgccctc cgagacccca cgaaatctgt tggcgcgccg gcagccggaa
     6721 gcggtgtacg aaatcgaggt cgaaggtgtc gcgaagctcg ccgaggttcg gctcatgacg
     6781 attcgtccgg tcgtagtaag cctgaacctg gatatcgctg ccctctccca gggttcgctt
     6841 ccagcggccc atgatattgc cgccggaaag aagcgcgttg gcatcgacga tttgtgaata
     6901 aggctgcgtg tagctggtgg cttggacgct ttcgccggct acctcgtcgt acagatcgcc
     6961 ttgcaaggtg aaggtatcgc gagtgttctt ttcccagtcc atgcgaaaac cggcctgggc
     7021 ggatcgccaa tcgtcgaagt ttctgtgatc cggatgaact tccgggcttc tggtgaaccc
     7081 tttgccataa aaacgatagt taaagccacg gccgttaccg cctccataac ggaaactacc
     7141 gaaagcctgt tcttcatttc caccaccggc ggacgccaaa aatccgcggg tatccttgga
     7201 gtccctggta atgatattga tcacgccgtt cacggcgttc ggtccccaga tggttccgcc
     7261 agggccgcgg atgacctcga tgcggtcgat gtcttccatc atcgtgtcct gcacttccca
     7321 gtaagtgcca tcaaagagcg gagtgtagac cgtgcggccg tcgatgagca cgagaacaga
     7381 tcgggtgagg cgggttccaa agccgcga
//
LOCUS       DATNDT010000446         5680 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2688831, whole genome shotgun sequence.
ACCESSION   DATNDT010000446 DATNDT010000000
VERSION     DATNDT010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5680)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5680)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5680
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2688831"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..393)
                     /locus_tag="VN788_14570"
     CDS             complement(<1..393)
                     /locus_tag="VN788_14570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ domain-containing protein"
                     /protein_id="HXU21804.1"
                     /translation="MAKRDYYEVLGVGRDADEQALKGAYRKLAMSYHPDRNPEDKNAE
                     EKFKEAAEAYAVLSDPQKRTAYDRYGHQGVAGAAQGFDPSSFTDFSDILGDLFGFGDL
                     FGGRGRGRNRAQRGDDLQYDLEIGFEDAM"
     gene            complement(396..932)
                     /gene="grpE"
                     /locus_tag="VN788_14575"
     CDS             complement(396..932)
                     /gene="grpE"
                     /locus_tag="VN788_14575"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HXU21805.1"
                     /translation="MKSAAEPGEESRGAAEDAETGSASRLQAVVAERDLLAAEKAGLQ
                     DRILRLQAEFENFRRRVEREKGEILEFANAETVRALLPILDDFERALKTESVDKEYAR
                     GMELIYQRLFDAMKKLGLEPIASAGETFDPHIHHAVEMVESDEHEDHAILDEYQRGYN
                     FRGKMLRPAMVKVAVRKS"
     gene            complement(987..2033)
                     /gene="hrcA"
                     /locus_tag="VN788_14580"
     CDS             complement(987..2033)
                     /gene="hrcA"
                     /locus_tag="VN788_14580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HXU21806.1"
                     /translation="MSISEFRQSSPSRHDEILHLIVQSYIESGEPVSSRIISRRLRRS
                     LSAASVRNVMADLYEQGFLSQPHTSAGRVPTAKAYRTYVQSLARAKILNSEFERLREE
                     LRRLGSLEARVERSSHLLTEITSGIGIAVAIPALAQSLDRIELVELADRRVLVVLITR
                     DGMVRNRVVVLEDGFAQDDLISIRNYLNRNFAGWPLPSIHTELKRRLEQESAAYDAIL
                     RRLTVLYAKGLLDVGFSPEIHLEGASNLVGLDLHLTRERMRELFRALEEKKKILHLLD
                     RFLVEVDGEVAVQVGLGDAHPSMGELSLIGLAIPLAGGLSAKIAVLGPMRMNYQRAMT
                     AVRHLGDAIQSIPS"
     gene            2161..2565
                     /locus_tag="VN788_14585"
     CDS             2161..2565
                     /locus_tag="VN788_14585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HXU21807.1"
                     /translation="MRKVIVWLLRVYSYLFHLLLSLFLVGIAIVSLASRPQTLKLGML
                     PWTGPGLSWWVLGLGIVGLICIFLAVTGIVRFLFPLWTLLIFILMLRGFFLSPFTFSG
                     AAQFRGAIWLTIGALVAFLGSLTLLIKRRRSN"
     gene            2801..3289
                     /locus_tag="VN788_14590"
     CDS             2801..3289
                     /locus_tag="VN788_14590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21808.1"
                     /translation="MTNVPRVAFFTDSFHEINGVALTSRQLDVYARERGLPMFSVHAG
                     PETGLTTNGSNCTLELKRTRASIALDRGDLAFDPFFARQLRRIKLDLQKFRPDLVHVT
                     GPGDIGILGAWLARRLRIPLVASWHTNLHEFAARRLEKLLDSFPAKTASHIGNFAERR
                     SL"
     gene            3323..4018
                     /locus_tag="VN788_14595"
     CDS             3323..4018
                     /locus_tag="VN788_14595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21809.1"
                     /translation="MLAPNQELIDVLQSSTGRPTFLMERGVETDIFSPLRRDCPTDGP
                     FVLGFVGRIQAEKNVRFLATLEQALISAGKRNFRFLIVGEGGERDWLKRHLLQADFPG
                     QLIGEPLARAYANMDLFVFPSLTDTFGNVILEAQASGVPAVVTSSGGPKFLVKSGLTG
                     FIAADESSFIESVLRVVNDPALHCRMREAAREQALRRSWPEVFEKVYSIYRYSLHQLT
                     LCSSSCVSLPQSR"
     gene            4164..>5680
                     /locus_tag="VN788_14600"
     CDS             4164..>5680
                     /locus_tag="VN788_14600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21810.1"
                     /translation="MATNQVLLQTRYLDLFENAHDAIYTHDLNGGILSWNRAAERLSG
                     YSRSEIVRKSIFDLLAPDSLALAREKLHILASGVVPPVYELVFVEKEGSRRTLEVSAH
                     LVWEAGRPAVVECIARDITERKRAEIELQLAKEKAEELSMAKSRFLAYMSHEIRTPLS
                     GVIGMSNLLLETTLNEQQQEYSETIQRSGDALLTVINDVLDFSKIEAGRMILESGRFS
                     IRKELASLLSLFGSQTRAKRLNMTLEVDPRISDALEGDAARLRQVLINLVGNAVKFTD
                     RGGIELRCELLQETAEGPLIRFEISDSGIGIPADSIGTLFDPFTQANGSVARYGGTGL
                     GLSISRQIVELMGGNITLDSEDGAGSTFSFAIVLKHATETAAVGQTVNALETARASAK
                     RGAIVLLADDSVVSQTVCLRLLEKLGCDVDVASEGATAVEKWRARRPDLVLLDCQMPG
                     IDGYEAATRIRQLESNGNHTPIVALTANALGGDREKCLAAGMDDYVAKPLQLEQLR"
BASE COUNT         1196 a         1626 c         1543 g         1315 t
ORIGIN      
        1 catggcatct tcgaagccaa tttcgagatc gtattgcagg tcgtctccgc gttgcgcccg
       61 atttcgcccg cgtcctcttc ctccgaacaa atcaccgaat ccgaacagat cgccgaggat
      121 atcgctgaaa tccgtaaaag atgacggatc aaatccctgc gcggcaccgg cgacaccttg
      181 atggccatat cggtcatacg cggtccgctt ctgcggatcg ctcaatacgg cgtaggcttc
      241 ggccgcttct ttgaacttct cctccgcatt cttgtcctcc ggattccgat ccggatggta
      301 gctcatcgcc agcttgcggt aggcgccctt gagcgcctgt tcatcggcgt cccgcccaac
      361 gccgagcacc tcgtaataat ctcgcttcgc cacgttcatg atttccgcac cgcaactttt
      421 accatcgcgg gccgcaacat ctttccgcga aaattatacc ctcgttgata ttcgtccaga
      481 atcgcgtggt cctcgtgttc gtcgctttcc accatttcca ccgcatggtg tatatggggg
      541 tcgaatgtct ctccagccga cgcaatcggt tccaagccca gtttcttcat cgcgtcgaat
      601 agacgttgat aaatcagctc catcccgcgc gcatattcct tatcgacgga ctccgtcttg
      661 agcgcacgct cgaagtcatc cagtataggc agcagcgctc ggacggtctc cgcattcgca
      721 aactcgagaa tttcgccttt ttcccgttcc acgcgccggc gaaaattttc aaattccgct
      781 tgcagcctga gaatccgatc ctgcaggccg gccttttcgg cggctaagag atcgcgttcc
      841 gcgacaaccg cttgcaatct ggaggcgctg ccggtttccg cgtcttcggc cgcgccgcgc
      901 gactcttcac cgggttcggc ggcggatttc atctgttcca tatccattat tttacgagct
      961 aaacgggaat aggcatccaa cgcgcgctat gacggaatac tttggattgc gtcgccaagg
     1021 tggcgaaccg ccgtcatcgc tcgttgataa ttcatacgca tcgggccaag taccgcgatt
     1081 ttcgcggaca aaccgccggc cagtggaatg gcgagaccga ttagcgatag ttcacccatg
     1141 ctggggtgcg cgtcaccgag gcccacctgt actgctactt ctccatcaac ctcaacaaga
     1201 aagcgatcga gcagatgcaa aatcttcttc ttctcttcaa gagcacggaa taactctcgc
     1261 atccgttcgc gcgtcaggtg caaatcaaga cccacaagat tgctcgcgcc ttcgagatga
     1321 atctcgggcg agaatcccac atccagcaga cctttcgcgt acagcacggt gaggcgacgc
     1381 aaaatagcgt catacgcggc actctcctgc tccagccttc gcttaagttc cgtgtggatc
     1441 gagggtaacg gccatccagc aaaatttcgg ttcaggtagt tgcggatcga aatcaggtcg
     1501 tcctgcgcga agccatcctc cagaactacc acccggttcc gcaccatgcc gtcgcgcgtg
     1561 atgagcacga ccaacacgcg ccggtccgct agttccacca gttcgatacg gtccaggctc
     1621 tgagccaacg ctgggattgc aacggcgatc ccgatgccag acgtgatctc agtcagtaaa
     1681 tgcgatgaac gctccacccg tgcttcgagt gaaccgagcc gtcttaactc ttcccgcagc
     1741 cgctcaaatt ccgagttaag aatcttcgcg cgggcaagcg actgtacata agtcctgtat
     1801 gccttcgcgg tgggaacacg cccagccgac gtgtggggct gcgataaaaa cccctgttcg
     1861 tacaaatccg ccatgacatt acgcaccgac gcggcgctga gggatctacg aagccgccgc
     1921 gagatgatgc gcgaagaaac cggttctcca ctctcgatgt acgattgcac gatcaagtgc
     1981 aggatctcat catgacgcga gggcgaggat tgcctgaact ccgatatcga catcgctgat
     2041 atacacaaac ccttatccgt tattgtaacg gtgcggcttt cacggggtca acaaggggcg
     2101 agctgttcca ctctgctatc caaccagtta tacaatagcg atttcgcctc aggaggagaa
     2161 ttgcggaaag tcattgtttg gctgctgcgc gtttatagct atctgttcca tctgttgctc
     2221 tcgctctttc ttgttggcat cgcgattgtt agccttgcca gccggcctca aacgctgaaa
     2281 ctcgggatgc tgccgtggac tggccccggc ctttcctggt gggtgctcgg cctcggtatt
     2341 gtcggattga tatgcatctt tcttgccgtc acaggcattg tccgctttct ttttccattg
     2401 tggacgctgc tcattttcat cctcatgctg cgcggctttt tcctgtcgcc attcacgttc
     2461 agcggcgccg ctcagttccg gggtgctatc tggctcacta ttggcgcgct cgtggcgttc
     2521 ctcggcagtc tcactctctt gataaagcgc cgccgcagca attaaacgtc tttcggaagg
     2581 aggcgtttca tctaatattt gcaacagaga agatcccgag cagtactctg gacgcgcgat
     2641 ggacgcattt tcatggatgg caacgcaacg tccttcccac gtttctgcaa aagggctggc
     2701 cccggcctgg tctagggttt accggcgcaa tgcggtatca aaagccagca tatccgatcg
     2761 atactgcgcg cggccttacg gaacgcgaga agatgacctt gtgaccaatg ttccgagagt
     2821 cgcgttcttc acagactcat tccatgagat caatggcgta gccctcacga gccgacagct
     2881 tgatgtatat gcgcgggagc gcggactgcc gatgttcagc gtccacgcgg gtccggaaac
     2941 cggattaacg actaatggtt cgaattgcac gctcgaactg aaacggaccc gtgcgtcgat
     3001 tgccctcgat cgcggagact tggcgttcga tcctttcttc gcccgccagt tacggaggat
     3061 taagctggat ctgcaaaaat tccgtcccga tctcgtgcat gtcaccggac cgggcgatat
     3121 cggaattctt ggcgcgtggc tggcgcgacg cctgcgaatt ccgctagtcg cttcctggca
     3181 cactaacctg cacgaattcg cggcgcggcg ccttgaaaaa ctgctagact ctttccctgc
     3241 gaaaacagcc agccatatcg ggaatttcgc agagcgccga tcgctttaga ttatcctctt
     3301 cttctacaag cgggccagcg tcgtgctggc tcccaatcag gaactcattg atgtccttca
     3361 gagcagcacc ggcagaccga cgttcctgat ggaacgaggg gtggaaaccg acatcttctc
     3421 gcctttgcga cgcgactgtc caaccgatgg gccatttgtg cttggcttcg tcggccgaat
     3481 tcaagcggag aagaacgtcc gttttttggc gaccctcgaa caggcgctca tttcggcggg
     3541 caagcgaaat ttccgttttc tgattgtcgg cgaaggagga gagcgggatt ggctgaaaag
     3601 acatctgcta caagccgatt ttcccggtca gctgattgga gagccgttag cgcgagcata
     3661 tgccaacatg gacctgttcg tttttccttc gctcaccgat acatttggca acgtaattct
     3721 tgaggcacag gcgtccggtg ttcccgccgt ggttacttcg agcggcgggc caaagttcct
     3781 cgtgaaatct ggcttaactg gctttatcgc cgccgacgaa agttccttta tcgaatccgt
     3841 cttacgtgta gtaaacgacc ccgctctcca ctgccgaatg cgggaagccg cccgcgaaca
     3901 ggctttgcgc cgttcctggc cggaagtctt cgaaaaagtg tactctatat atcgttactc
     3961 tcttcaccaa ctcacattgt gttcgtcctc ttgtgtatcc ctcccacaat cgcggtagat
     4021 tgaattatct tttcgacgta caataaactg gccgtattgt gacttcgtgg atcaccacta
     4081 ttgcgtgcct gttgggagcc gctggatggg tctttgctct tgtccattat cgtctagcac
     4141 gccgcgcctc gcgggaaaga actgtggcca ctaatcaggt tctattacaa actcgatatc
     4201 tcgatctgtt cgagaatgcg cacgacgcaa tctacactca cgatctgaat ggagggatcc
     4261 tctcctggaa ccgcgcggcg gagcgcttga gcggatacag ccgctccgaa atcgtgcgga
     4321 agtcgatttt cgacttgctt gcgccggact ctctcgcttt ggcgagggaa aaacttcaca
     4381 ttctggcgag cggtgtcgtg ccaccggtat atgaactggt attcgtcgag aaagagggtt
     4441 ctcggcgcac gctcgaagtc agcgctcacc tagtgtggga agcagggcgg cccgcggtgg
     4501 tggaatgtat cgcgcgggat attacagaac gaaaacgggc tgaaatcgag ctccaactcg
     4561 cgaaagaaaa agctgaggaa ctttccatgg cgaagtcgcg attcctcgcc tatatgagcc
     4621 acgagatccg cactccgtta agcggcgtga tcggcatgag caaccttctg ctagagacca
     4681 cgctcaacga gcagcagcag gagtattccg aaacgatcca gcgttcgggc gatgcgctgc
     4741 ttacggttat caacgatgtt cttgattttt cgaaaatcga agcgggccgg atgattctgg
     4801 agtcgggccg attctcgatt cggaaggaac tcgcaagttt gctgagcctg ttcggatcgc
     4861 agacacgggc gaagcggctc aacatgaccc tggaagtgga ccccagaatc tcggatgccc
     4921 tcgaaggaga cgcggcgaga ctgcggcaag tgctgattaa tctggtcggc aacgctgtaa
     4981 agttcacgga tcgaggagga attgaactcc gttgcgaact gcttcaggag accgcggagg
     5041 gaccgctgat ccgttttgaa attagcgatt cgggaatcgg aatccccgcc gattcgatcg
     5101 ggacgttgtt cgatccgttc acacaggcga atgggtcggt tgcgcgctac ggcggcacgg
     5161 gccttggact ttcgatttcg cggcagattg tcgagttgat gggcggcaac atcaccctcg
     5221 atagcgagga cggcgccgga agcacttttt cgttcgcgat tgtcctcaag catgctactg
     5281 aaacagcggc ggtggggcag accgtgaatg cgctagaaac agctcgcgcc agcgcgaaga
     5341 gaggcgcaat cgtgctactg gcggatgata gcgtggtcag tcagacggtg tgcctgcgcc
     5401 tgctcgagaa actcggttgt gacgtagatg tcgcttcgga aggagcaact gccgttgaga
     5461 agtggcgcgc ccgcagaccc gatctagtgc ttctcgattg ccagatgcct ggcatcgatg
     5521 gatatgaagc agcgacccga atccgccaac tcgaatccaa tggaaatcat acgccgattg
     5581 tcgcgctcac agcgaatgcg ctgggtggag atagggagaa gtgcctggcg gcggggatgg
     5641 acgattatgt ggcaaaaccg ctgcaattgg agcaattgcg
//
LOCUS       DATNDT010000447         3295 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4250645, whole genome shotgun sequence.
ACCESSION   DATNDT010000447 DATNDT010000000
VERSION     DATNDT010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4250645"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VN788_14605"
     CDS             <1..343
                     /locus_tag="VN788_14605"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HXU21811.1"
                     /translation="ERRFFLASQVTETIVANESSPLRDLYVVYAGRSPDTNKPVIHAY
                     LNPLVKWIWFGGIVVVLGTGLAMIPDRRAALVLRAASEKSWGSSVAPSVGGSPASSPV
                     PTFPIRRSFPE"
     gene            complement(447..522)
                     /locus_tag="VN788_14610"
     tRNA            complement(447..522)
                     /locus_tag="VN788_14610"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(487..489),aa:Val,seq:cac)
     gene            584..1552
                     /locus_tag="VN788_14615"
     CDS             584..1552
                     /locus_tag="VN788_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXU21812.1"
                     /translation="MQTATNTKTNAFSGRLADLQRSIAQIVKGKEAVIQLALTALLAR
                     GHLLIEDVPGVGKTTLAQALARSFQCSFQRIQFTSDMLPGDVIGVSIFNPASRNFEFK
                     PGPIFANIVVADEINRTTPKTQSALLEAMNESQITVDNHTHPLPEPFMVLATQNPIEH
                     HGTYPLPESQLDRFVMRIRMGYPSRASEKEIIRNHSAARRAEGIAPLMDAADVAQMQR
                     AVSDVKVDDSLLDYAIEIVERTRQTEQLSLGVSPRGTVMLHRAAQARAFVEGRDYCLP
                     DDFKQLIVPVFAHRVVVSSRYVSTQKKSEQSEAILGEILETTRVPL"
     gene            1702..3012
                     /locus_tag="VN788_14620"
     CDS             1702..3012
                     /locus_tag="VN788_14620"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXU21813.1"
                     /translation="MQDSITRLWRRRDRPGWRGFGIAMLALSVALVLALFSAAVAQEG
                     RVWLAAVTTLIALGIAAWVAIAIVPALAKRTNLRWIAYQVDYRLTREGVIYLLAVIVL
                     VFAAVNTGNNLLFLVLACLLAGILVSGVLSRIVLTGIAMKCDLPEHIFAGQPVLAEIE
                     LRNEKQIWPSFSLRVLGASKKSTTKILTCPVFFPYIPRFSAARQKVELRFQQRGVYRQ
                     DAFGIRTRFPFGFFEKTRQVESNMEITVYPPVEPTDQFYEVLPLLSGEMASYFRGRGH
                     ELHSLRDYQPTDSGRLVDWKVTAKSGRLMVREFAREDERRVMLVLDPYIGKVAPAEHM
                     ARFERAVSLTACIAWHFNEIHSVMQFRTGSYSTPMAPAVEIIYHTLRELALVQPEISE
                     VEGASLDSLGGDREIFKIILTARAQQSIPAALWSTSYFIFMDRL"
     gene            3089..>3295
                     /locus_tag="VN788_14625"
     CDS             3089..>3295
                     /locus_tag="VN788_14625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005008368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HXU21814.1"
                     /translation="MRFPLAVGYRSGIQRSLLAWFRAHRRELPWRANRDPYRIWVAEI
                     MLQQTRIAVVEPYYDRFLKSFPSID"
BASE COUNT          673 a          957 c          927 g          738 t
ORIGIN      
        1 cgagcgccgc ttcttcctgg cgagccaagt caccgagaca atcgttgcca atgaaagcag
       61 cccgctgcgc gacctatacg tcgtctatgc cggccgcagc ccggacacaa ataagccggt
      121 gatccacgcc tacctgaatc cacttgtgaa gtggatatgg ttcggcggca tcgtggtagt
      181 tcttgggaca ggcttggcca tgattcccga tcgtcgggcg gccctggtat tgcgcgcagc
      241 ttccgaaaaa tcctggggca gctcggtcgc cccaagtgtc ggcggctcgc cagcttccag
      301 ccccgtgccc acgttcccca tccgccgctc atttccggaa tagcctctca gcactgcaaa
      361 tgcgcctcct gaccacttct tgggcacacc acagcaaatc agggtttcag acagccgatc
      421 acttgatggc agaatcaagt ttgggatggt gggcgaccaa ggacttgaac cttggacctc
      481 tcccgtgtga ggggagcgct ctaaccactg agctagtcgc ccgcaaagcg tgacgagagt
      541 cagcctcgtc taggcgcttc atttttgcaa aaggccaggt caattgcaaa ctgccacaaa
      601 cacgaaaacg aacgctttca gcggaagact cgccgacctt caacggtcaa ttgcgcagat
      661 cgtcaagggc aaggaagcgg taattcagct tgcactgacg gccctgctgg cacgaggaca
      721 tttgctgatt gaagacgttc cgggcgtcgg caagacaacg ctggcgcagg cgctggcgcg
      781 ctcgttccaa tgctcgtttc agcgcattca attcacatct gacatgctgc ccggcgacgt
      841 aatcggcgtc agcattttta atccggcatc gcggaatttc gaattcaagc ctggtccgat
      901 ctttgccaac attgtggtgg ctgatgaaat caaccggacc actcccaaga cgcaatccgc
      961 gttgctcgag gcgatgaacg aatcgcagat caccgtggac aaccacacgc atccgctgcc
     1021 tgagccgttc atggtgctcg ccacgcaaaa tccgatcgaa catcatggta cgtatccgct
     1081 gcctgagtcg cagcttgacc gtttcgtgat gcggatccgc atgggctatc cttcgagagc
     1141 cagcgagaag gaaattattc gcaatcattc cgccgcaaga cgcgcggaag gaattgcgcc
     1201 gttgatggac gctgccgatg tggcccaaat gcagagagct gtttcggacg taaaagttga
     1261 cgacagcttg ctcgattatg cgatcgagat cgtggaacgt acgcgtcaaa ctgagcaact
     1321 cagcctaggg gttagcccac gcggcaccgt aatgctgcac cgcgcggcac aggcgcgggc
     1381 ctttgtcgag ggccgcgatt actgcctgcc ggacgatttc aaacagctga tcgtgccagt
     1441 cttcgcgcac cgagtcgtgg tgagttcccg ctacgtctcg acacagaaaa agtcggagca
     1501 gagcgaagcg atccttggcg aaattttgga aaccacgcgc gtaccgctgt gaaaaaggct
     1561 gtttcgcggt tttaacgcaa ataccacgcg acctcggtta aagccgcgcc aagaaaggcg
     1621 ccgcacggat acgcctcggt ttcggtttgt tcgtctgaca tcccttgggg ttaactggac
     1681 gagtcgccgg taaaaacaat catgcaagac tcgattacgc gcttgtggcg ccgccgagac
     1741 cgtcccggct ggcggggctt tggtatcgcc atgctggcgc tttcggttgc cttggttctt
     1801 gcgcttttct ctgccgctgt cgctcaggaa gggcgcgtct ggctcgcggc cgtaaccacg
     1861 ctcatcgccc tcggaatcgc ggcgtgggtg gcgattgcga tcgtgccggc gcttgcgaag
     1921 cggaccaacc tgcgctggat cgcctaccaa gtggattatc gtttgacgcg cgaaggcgtg
     1981 atttacctcc ttgctgtcat cgttctcgtt tttgcggcag ttaacactgg aaataacctg
     2041 cttttcctcg tcctcgcctg tttgcttgcg ggcattctgg tttcgggagt gctctcccgc
     2101 atagttctga ccggtatcgc catgaaatgt gacttgcccg aacatatttt cgccggtcag
     2161 cctgtgcttg ctgagattga gctgcgaaat gaaaaacaga tctggccctc cttttctctc
     2221 cgcgtgctag gtgccagcaa gaaatcaacg accaaaattc tgacctgccc cgtcttcttt
     2281 ccctatattc cgcggttcag cgccgcgcgg caaaaggtgg aactgcgctt tcaacagcgt
     2341 ggcgtttacc ggcaggacgc gtttgggatt cgcacgcgtt ttcccttcgg cttcttcgaa
     2401 aaaacgcggc aagtcgagtc gaatatggaa atcaccgtct acccgcccgt ggagccgacc
     2461 gaccagtttt acgaagtctt gccgctgctg agcggtgaga tggccagcta cttccggggg
     2521 cgcgggcacg aactgcactc actgcgcgat tatcagccga ccgatagcgg gcgcctcgtg
     2581 gactggaagg ttacagcaaa atcgggtcga ttgatggtgc gcgaattcgc tcgcgaagat
     2641 gaacggcgcg tgatgctagt gctcgaccct tatattggca aagttgcgcc agccgaacat
     2701 atggcgcggt tcgagcgggc tgtttcgttg actgcctgca tagcttggca cttcaatgaa
     2761 attcactcag taatgcagtt ccgcaccggt agttattcaa cacccatggc cccggccgtc
     2821 gaaattatct accatactct ccgcgagttg gcgctggttc agccggagat atcggaagtg
     2881 gagggcgcct ctctcgatag tctcgggggc gatcgggaga tttttaagat cattctcacc
     2941 gcgcgcgcgc agcagtctat tcctgcggca ctttggtcca cctcgtattt cattttcatg
     3001 gaccgattgt aatagtctca gtgctctcaa aacgctccga ggcctgaatc gcgtgatgcg
     3061 aacggctgcc cgtggcagaa taggcgccat gcgatttccg ctagccgttg gctaccggtc
     3121 cgggatccag cggagcctcc ttgcatggtt tcgcgcgcat cggcgcgagc tgccttggcg
     3181 ggcgaaccgc gatccctatc gaatatgggt tgccgagatc atgttgcaac agacgcgtat
     3241 cgcggtagta gagccttatt acgaccgctt cctgaaaagc ttcccttcaa tcgat
//
LOCUS       DATNDT010000448         6187 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3555353, whole genome shotgun sequence.
ACCESSION   DATNDT010000448 DATNDT010000000
VERSION     DATNDT010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3555353"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1106)
                     /locus_tag="VN788_14630"
     CDS             complement(<1..1106)
                     /locus_tag="VN788_14630"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HXU21815.1"
                     /translation="MNVYTLAVHEDVTYWTPRATVGEVSIHDQMIDLNSGLLGRLVES
                     NSVILRSGTAIKREDYPHIDVRRTLSSLCYVPLMNGDLLGTLEILSFRENLTAESVAA
                     LQPAAQLAAAAIVSAQSYEEERHETLTSITRLTQLYDLEKAFSSTLEMEDLLPLIGSK
                     FSEILSAQAVNIWLLHPDETLELMHQNGSDPTTFTGQILNPNEGVAGIVSDNGEGMCI
                     SDATDPRLVARNAAAGEPSVRSLLAAAIIDRGSLVGVVEAVNKSDEGNFGEDDLFTLT
                     SLNDTASTALHNAGLLMAERKVEILETLNTVSHEITSTLNLERMLQTIVNAPQAVIPY
                     DRASLALDQHGRFKLSAVTGFTKIDAESPELSPI"
     gene            1405..1743
                     /locus_tag="VN788_14635"
     CDS             1405..1743
                     /locus_tag="VN788_14635"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU21816.1"
                     /translation="MGIETNTQGELRIVKVTGRLSMGPELDRFTAALDGLLNRSHGKI
                     ILDLEEVPTIDSSAIGVLVRYLTSAKKMGGSIKLLKPTKFVVQTLKMVGLLNLFPAYE
                     DLTEALASFH"
     gene            1967..4861
                     /locus_tag="VN788_14640"
     CDS             1967..4861
                     /locus_tag="VN788_14640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21817.1"
                     /translation="MGTTVAFVLVLVFAQTVSAQTNQLNFGENYFVSGDYVVAGVGLR
                     GLGGKNGLATSTLTMPDTTSVPSTGVPAGADIVAAILYWQTVESTQTQFVGQNGFFRP
                     MFKGGPATGYAITGVVLGNPNAPTSWSSGGCSGSAQGSKTIRTYRADVRPFLPQDANG
                     NILAGNKQAPVTYEVSLPDGGKSGATPITLGASLVIIYRALTPPSSVNPPSLPLNSVV
                     IYDGAAAPNNSSSTFSQTIQGFYQAAASPISKLTHIVGNGQNNKSETVSFDGQSLPSL
                     YANLPPFPGFYNGSWDNPTWSFPNANYLNNTNPVMANDSSAITMVAPSASNSGCVSWG
                     AVIVSTTVQDSDKDGLLDKWESDKGYCDASVNEGACTRNDPSWVSLPDADPAKRDIFV
                     QMDYMCSIVNADGTCDTTNGYSFLPDPNARAMMVTAFSNQNVNLHLISTNAIQEPTCT
                     DDLSTNPPALCFFPNQPGVVDWKNGLEFLKEQPLNYPDENSCEQALNGPCTRRFQHGR
                     KDSYHYALFAHASATPSWGFVAGNLTSVVASGTTVTFTTSTPHGLAPNDTVDGNGRVT
                     VIGAISNFNLNGTYYVKTTPTPTTFTIQIANATSANPTFSTDPGLWVFSGQAGTGSGI
                     SDVGGADSLISLGSWGADGQSVSVQAGTFMHELGHSLGLTHGGIYYDTQGTYIPTIES
                     NCKANYQSVMSYLFQVDLLDNGVLDYSEQGLTSLNERSLPLGLTTTDGSTLAYSTTKW
                     YLPNAPFGVGTAATHHCDGSPVSSSDVDMTMYRAEGSANPVAPAWVNRSDTNFDGTLD
                     TNLRGYNDWTSVDLRQIGATANDIGSPGISHFGTGISHFGTGVSHFGTGVSHFGTGVA
                     EIDRQTANSFTRPPRKLTATVIGPPNAIQLNWNVPTFGQIATYNIYRGVGTGVNPAPP
                     AYATVAGSQTSFVDTNISCGGTYTYFVTALLSNGQESVASNLVTVVKCGR"
     gene            complement(4895..>6187)
                     /locus_tag="VN788_14645"
     CDS             complement(4895..>6187)
                     /locus_tag="VN788_14645"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU21818.1"
                     /translation="GNTEEALRSYSKAIALDPNFARAYGGMSAAYANLGKTQDAEKYA
                     KLAMEHVDRMTERERYRVRAFYYFTMGDWQKCVEEYGELVKQYPADNVGQANLSGCFV
                     ELRNFPKAVEAARQAVQIAPQGALQRLNLSFNSSYAGNFKAGEQEARTALGLNRSSRA
                     YLALAESQLGQGQMDQATETYHQIEKMGAEGASLAASGLADVAAYEGRYAYAARTLEH
                     GAAADLKAKSPDNAAEKLASLSHIEFVRGDTAVAVADATKALSASQTVPVRVLAALTF
                     VESGQTAKAQTLAAGLASELQAEPQSYAKIIEGMIAAKRGDNRQAIATLTDATKLFDT
                     WISRFELGQAYLEAGLFVESDSEFDRCIQRRGEVLELFMDDTPTYGYLPDVYYYQGRV
                     REGLKSPGFAEPYRMYLGIRGKAGEDPRLAEIRRRISR"
BASE COUNT         1384 a         1723 c         1697 g         1383 t
ORIGIN      
        1 tttatgggcg agagttccgg cgattccgca tcgattttcg tgaagcccgt gacggcgctg
       61 agtttaaagc ggccgtgctg atccagggcc agcgacgcgc gatcgtatgg gatcaccgct
      121 tgaggtgcgt tcacaattgt ctgcagcatg cgttcgagat ttagggtgga agtgatttca
      181 tggcttacgg tattgagcgt ttcgagaatc tccaccttgc gttcagccat cagtaagccc
      241 gcattgtgca gcgcagtgga cgcggtgtcg ttcaagctcg tcagagtaaa aagatcgtct
      301 tcgccgaaat tgccctcatc ggatttgttt actgcctcga cgacacccac aagagatcct
      361 cgatctatga tcgcagccgc caatagcgat cgtacggacg gctcccccgc cgccgcgttg
      421 cgcgctacga gccgcgggtc cgtcgcatct gaaatgcaca ttccttctcc gttgtcagag
      481 acaatccccg ccactccttc attgggattg agaatctgtc ccgtgaatgt ggtcgggtct
      541 gatccgttct ggtgcatcag ttcgagagtt tcatccggat gcaagagcca gatatttacg
      601 gcttgtgcgc tcagaatctc ggagaacttg gatccgatga ggggtaacaa gtcttccatt
      661 tcgagagtcg acgaaaatgc tttctccaga tcataaagct gcgtcaggcg agtgatggat
      721 gtaagtgttt catgccgttc ttcttcgtag gactgtgcgg acacgatggc ggctgcggcc
      781 agttgggccg cgggttgcaa ggccgcgacg gactccgcag tgagattctc gcggaaagaa
      841 agaatttcca gggttcccag taggtcacca ttcatcaagg gaacgtagca aagcgaagac
      901 agcgtcctgc gcacatcaat atgtggatag tcttcccgct ttatggctgt tccgctgcgc
      961 aaaatcactg aattactttc taccagccgg ccaagtaaac cggagttcaa atcgatcatt
     1021 tggtcatgaa tgctaacctc gccaaccgtc gccctgggcg tccaataggt gacatcctca
     1081 tgcaccgcaa gcgtgtacac attcacggcg gactcgggaa aaagttcggc aacgaaacga
     1141 gcgagcgaac gagcgcgggg ggctacctct tggtctgcta gtagactgga agagagcgaa
     1201 gaaaaatcca gactcgattc ttgcttacgg cttaacaacg ttcgagcctt ccgcccgttc
     1261 actcgttcgg cgagaccgcc cgggggactg agttccttgc attttgggct gagattaact
     1321 aaaacttgag cgcgcgcact ctaacaaagg tatctgcgag tgtcaacgac ttgggcatcc
     1381 gagtccacgc tccgaggaga aactttgggt atcgaaacga acacgcaggg agaacttaga
     1441 attgttaagg tcacgggcag actttctatg gggccggaac tggatcggtt caccgcggct
     1501 ctggatgggt tactaaaccg ttctcacggc aagatcattc tcgacctcga ggaggtgccg
     1561 accatagact catccgcgat cggcgtgctg gttcgttacc tgacgtccgc aaaaaaaatg
     1621 ggcggatcga ttaaactgct aaagcctacg aaatttgttg tgcagactct caagatggtc
     1681 ggattgctga accttttccc cgcctacgaa gatctaacgg aggcgctggc ctcgtttcac
     1741 tgacgcgacc tcacgacaac ccccccgcca attcaggtac catcagtcta gaaaaagacg
     1801 tagatctgga actcttagta cccctttttg acccgcctga ttgcattttt cgatttgact
     1861 ttgcgcgcca atctggcaac atggcgcgtc ccctaatccc aaagatgcgg tgatcaagtt
     1921 taaggaggtt accatcctgc gcacagcttt gtcggctgcc cggttaatgg gcacaacggt
     1981 cgcgttcgtg ttggtcctcg tgtttgcgca gacagtgtcg gcgcagacta accaattgaa
     2041 tttcggcgag aactatttcg tcagcggcga ttacgtcgta gctggcgtgg gcctgcgcgg
     2101 acttggggga aagaacggct tagccaccag tacgttgacc atgccagata caacttctgt
     2161 tccctctaca ggggtacccg ctggggccga cattgtagcg gcgatcctct attggcagac
     2221 agttgaaagt acgcagaccc aattcgttgg acaaaacgga ttcttcagac cgatgttcaa
     2281 aggcgggccg gccacggggt acgcgattac cggtgtggtg ttgggaaatc cgaatgctcc
     2341 tacatcctgg agttcgggcg gttgttcagg gagcgcgcaa ggttcgaaaa cgatccgcac
     2401 ctatcgcgcg gacgtgcgtc cgtttctgcc gcaagatgcc aacggcaata tccttgctgg
     2461 taataagcag gcccccgtca cgtacgaagt gagcctgcca gatggcggaa agagcggggc
     2521 cacgccgata acgctaggag caagcctggt aatcatttac cgcgcactta ctcccccatc
     2581 atccgtgaat cctccttctt tacccttgaa ttccgtggtg atctacgacg gtgctgccgc
     2641 gccgaacaac agttcctcga ccttttcgca gacgatacag ggattctacc aggctgccgc
     2701 aagcccgatt tccaagctga ctcatatcgt cgggaatggc caaaacaaca agtccgaaac
     2761 tgtgtctttt gacggccaga gcctaccttc tctttacgca aatcttcctc ccttcccggg
     2821 tttctacaac gggtcatggg acaacccaac ttggagtttt ccaaatgcga attatctcaa
     2881 caacacgaat ccagtaatgg ccaatgatag ttctgcgatt acgatggtgg cgccgagtgc
     2941 ctcgaacagc ggctgcgtct cgtggggtgc cgtgatcgta agcacgactg tgcaggatag
     3001 tgacaaggac ggcctgctgg acaagtggga aagcgacaaa ggatactgcg atgcttccgt
     3061 gaacgaaggc gcatgcacga gaaatgatcc ctcgtgggtc agtctccccg atgcagatcc
     3121 agccaagagg gacatcttcg tccagatgga ttacatgtgc agcatcgtaa atgcggatgg
     3181 cacctgtgat accacaaacg gatattcatt cttgccggat ccgaacgcgc gggcgatgat
     3241 ggtcaccgcg ttctctaacc agaacgttaa cctgcatttg atatccacga acgcgattca
     3301 agaaccaacc tgtaccgatg atttgtcgac gaacccgccg gcgctgtgct ttttccctaa
     3361 ccagccggga gtggttgact ggaagaatgg tcttgagttc ctgaaagagc agccgcttaa
     3421 ttaccccgac gagaattcct gcgagcaggc cctcaatggg ccgtgtacac gccgattcca
     3481 gcacggacgg aaggatagct atcactacgc tctgttcgcg catgcttcag caacgccgag
     3541 ttggggtttt gtggccggaa acttgacgag tgttgtcgcc tctgggacga cggtgacgtt
     3601 cacgacctcg actccccacg ggcttgcccc gaatgatacc gttgacggca atggccgtgt
     3661 gaccgtcatc ggcgcaattt caaacttcaa cctgaatggc acttattacg ttaaaaccac
     3721 tccaacccct accacgttca caatacagat cgctaatgcg acaagcgcga accctacctt
     3781 ttccaccgat cctggccttt gggttttctc cggccaggct ggcactggat ccggaatttc
     3841 cgacgtcgga ggtgccgatt cgctgatcag tttgggttcc tggggagcgg acggtcagag
     3901 cgtgagcgtg caggctggca cattcatgca cgagctggga cattcacttg gtctgacgca
     3961 cggcggcatc tactacgata cgcaaggcac ctacatcccg acaatcgaat cgaactgcaa
     4021 ggccaactac cagagcgtga tgagctacct tttccaagtt gacctcctgg acaatggagt
     4081 cctggattat tccgagcagg ggttgacgtc gctcaacgaa cgcagcctgc cacttgggct
     4141 gaccacgact gatggctcaa ctctcgcgta ttcgaccacg aaatggtact tgcctaacgc
     4201 gccgtttggt gttggtacag cggcgactca ccattgtgat ggctcgccgg tttcgtccag
     4261 cgatgtagac atgacgatgt atcgcgccga aggttctgct aacccagttg cgcccgcttg
     4321 ggtgaaccgc tcggacacga atttcgacgg cacactcgat accaacctcc gtggctacaa
     4381 cgattggacg agcgttgacc tgcggcaaat tggagctacc gcgaacgata tagggagccc
     4441 tgggatttcg cactttggca ccgggatctc gcactttggc accggggttt cgcactttgg
     4501 tactggggtc tcgcacttcg gcaccggcgt cgcagaaatc gatcgtcaga cggctaactc
     4561 ttttacgcgg ccgccgagga aactaacggc gaccgttatc ggaccgccga acgccataca
     4621 actgaactgg aacgtgccaa ctttcgggca gatagccacg tataacatat accgcggcgt
     4681 gggcacgggc gttaacccgg cgcctccggc ttacgctaca gtggccggaa gccagacctc
     4741 cttcgtcgac acaaatatta gctgcggagg aacctacaca tatttcgtga ccgcgctgct
     4801 atcgaatggc caggagagtg tggcttcgaa cctcgtcact gtggtcaagt gcggcagatg
     4861 aaattgaaat cgggaacttg aagagaaaaa cgccctagcg gctgatgcgc ctgcggattt
     4921 cagccagccg gggatcttcg ccggccttgc cgcgaatgcc cagatacatg cggtacggct
     4981 ccgcgaaacc aggactcttc aatccttcgc gcacgcgtcc ctggtaataa tagacatccg
     5041 gaagatagcc ataggtgggt gtatcgtcca tgaagagctc gaggacttcg ccgcgccttt
     5101 gaatacatcg gtcgaattcc gaatcagact caacaaagag tccggcttcc aggtaggctt
     5161 ggcccaactc aaagcggctt atccaagtat caaaaagctt ggtagcgtcg gtcagcgtcg
     5221 caattgcctg ccggttatcg ccgcgcttcg cggcaatcat tccctcaatt atttttgcat
     5281 aggactgagg ctcggcctgt aactcggaag ccaggccagc agcgagtgtt tgcgctttgg
     5341 cggtttgtcc gctctcgaca aaggtaagcg ccgccaagac tcgaacgggc acggtctggc
     5401 tggcggatag ggccttggtt gcatcagcaa cggcgactgc agtatcgcca cgcacaaatt
     5461 ctatgtgaga aagagaagcc aatttctcgg cagcgttgtc gggactctta gcttttaggt
     5521 cggcggctgc gccgtgctca agggtccgtg cggcatacgc gtaccggcct tcgtaggcag
     5581 caacatccgc caaaccagaa gcggccaagg aggctccttc ggcacccatc ttttcgatct
     5641 ggtgataggt ttccgtagct tgatccatct ggccctgacc gagttgggat tcggccagag
     5701 cgagataagc tctggaagag cggttgagtc ccagcgccgt gcgcgcctcc tgctcgcccg
     5761 ccttgaaatt acccgcgtaa gagctattaa acgacagatt gagccgctgc agcgcgcctt
     5821 gaggtgcaat ttgcacagct tggcgcgccg cctcgacagc tttagggaaa ttgcgcaact
     5881 ccacaaaaca gccggagaga ttggcctgtc cgacgttgtc ggccgggtac tgcttgacta
     5941 gctctccgta ttcctcgaca catttctgcc aatcacccat cgtgaagtaa taaaaggcgc
     6001 gcacccggta gcgttcgcgc tcggtcatgc ggtccacatg ttccatagcc aacttcgcat
     6061 atttttcggc atcctgagtt ttgccgaggt tagcgtatgc agcagacatg ccgccatagg
     6121 cgcgcgcaaa gtttggatcc agggcaatag ctttggaata ggaccgcaga gcctcttccg
     6181 tgttccc
//
LOCUS       DATNDT010000449         4208 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3437817, whole genome shotgun sequence.
ACCESSION   DATNDT010000449 DATNDT010000000
VERSION     DATNDT010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3437817"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..818)
                     /locus_tag="VN788_14650"
     CDS             complement(69..818)
                     /locus_tag="VN788_14650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21819.1"
                     /translation="MATNVSGRRYRTWYAMLLRLYPRPFRERFGEGMAQTFHDLCQER
                     KGARRGLFAFALWIFFETSVGIVRENIMRMNQLGKTMLRVALGALAVLMVPLVASQFV
                     EGWNWPAGAFVRVYVLFFATGMVFALVARRMGALSYKAGVGVALVAGFALGWSTMVQT
                     AHSGHPERLWYLSVLVVGFIGACLARLKAAGLAVTLFAMAAVLALISVVLPSGAPSDM
                     ALRMAVGHGVFVALFTASGLLFRHASLAGLR"
     gene            complement(808..1155)
                     /locus_tag="VN788_14655"
     CDS             complement(808..1155)
                     /locus_tag="VN788_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXU21820.1"
                     /translation="MAKKETAQAPLTPAVLHILLALSTGERHGYGIMKQVEADSQGKV
                     KMGPGTLYGSMRRMMEAGLIRESDKRVDPEMDDERRIYYELTGAGRAALEAELKRYRG
                     VVAVAEGRLAHGH"
     gene            complement(2014..2688)
                     /locus_tag="VN788_14660"
     CDS             complement(2014..2688)
                     /locus_tag="VN788_14660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU21821.1"
                     /translation="MKVRLQFTKTLFLAGVLSTFAICSLADTISLNSSASETTNDSSS
                     ATLNIPKHPGWANPLGSSSWVSYGETGDPSAPGYFAPANGTVVTFTDMFFIGGTPAGG
                     SVNVLADDSTAVVLNGVTLFNEATSTGNGYGTCSDFGIGCLTSTEGHIDLTSGLTAGW
                     NTLSFAVAQRAGSSYGLDYSGSVDYSVDPNGGTSATPEPASMLLLATGLLGLGGFRRW
                     RKGQRG"
     gene            complement(2939..4147)
                     /locus_tag="VN788_14665"
     CDS             complement(2939..4147)
                     /locus_tag="VN788_14665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU21822.1"
                     /translation="MRSDSAPSRADSQAAVAACFLGWTLDAFDFFLVVFCLTAIGRDF
                     HKSDSDIVFSITLTLAFRPVGAFLFGMLADRYGRRLPLMLDLVFYSIVEVLTGFASSY
                     TMFLILRALFGIGMGGEWGVGASLAMEKVPPRLRGLISGFLQQGYAVGNLLAAICFFI
                     VFPRWGWRPLFFIGGLPALLALFVRVRVKESEVWRESRSLNWGDLTKTILSHWKLFVY
                     LTIFMMAMNCASHGTQDMYPTFLQRQFGFSARVRAVLSGISMIGAIIGGIGFGWLSDR
                     FGRRRSISLAMILAILVVPLWAYSSTLVLLAAGAFLLQFFVQGAWGVIPAHLAELSPN
                     EVRGFLPGFAYQCGVLLAASVAWLEAILAQKISYASAMALTAGLVFVFAAVMAWFGQE
                     RRGADFTRLI"
BASE COUNT          983 a         1340 c         1133 g          752 t
ORIGIN      
        1 cagataggcc cagaccattg ctggcgcatg tcgatccggg ccgaccgact ttgtgatcgg
       61 cagcggtctt acctcaatcc cgccaaactt gcgtgccgga acagcagacc tgacgcggtg
      121 aacaacgcta cgaagacgcc atgcccgacg gccattctta gcgccatgtc ggacggcgca
      181 ccggaaggta gcaccacgga aatcagcgcc aacaccgctg ccatcgcgaa caacgtgacg
      241 gccaatcctg ccgccttcag ccgtgccaga caagccccaa tgaatcccac tacgagcacg
      301 ctgagatacc acaggcgctc cggatgtccc gagtgcgccg tttggaccat ggtcgaccaa
      361 cccaaggcga acccggcgac caaggccacg ccgacgcccg ccttgtacga caacgcgccc
      421 attctcctcg cgaccagcgc aaacaccatc cctgtcgcga agaacaggac atacaccctc
      481 acgaaagctc ccgcaggcca gttccagccc tccacgaatt gcgatgccac gagcggcacc
      541 atcaacacag ccagcgcccc cagcgccaca cgcagcatcg tctttcccag ttggttcata
      601 cgcattatgt tctccctaac gattcccacc gacgtctcga agaagatcca cagcgcgaac
      661 gcgaacagtc cccgcctagc gcccttacgc tcctggcata ggtcgtgaaa tgtctgcgcc
      721 atcccctcgc cgaaccgctc acggaaaggc cttggatata gccgcagcag catcgcatac
      781 cacgtccggt agcgccggcc cgagacgtta gtggccatgc gccaaacgtc cttccgccac
      841 cgccaccacg ccgcgatacc gcttcagctc cgcttccagc gccgcccgcc ccgcgccggt
      901 gagctcgtaa tagatccgcc gctcgtcgtc catctccgga tcgacccgct tatcgctttc
      961 ccggatcagc cccgcctcca tcattcgcct catcgagcca tacagcgtcc ctgggcccat
     1021 cttcaccttg ccctgcgagt ccgcctccac ctgcttcatg atcccgtacc cgtgccgctc
     1081 ccccgtcgaa agcgccagca ggatgtgcag caccgcaggc gtcagcggag cctgcgccgt
     1141 ctctttcttc gccatgcttc gacactatat ccattacgga tatatgtcaa gcccaactga
     1201 gtgcccgagc agtttcttta aaaccagtgc ggtaattcct cccgtgcaaa ccggatatac
     1261 cccgtgccgg atatcgcctc gttaccgggc ttcacggatc gcttggaaag ggagcagggg
     1321 cattcggccc ggggctgtca gagagtgaac agcacacgtg ttggcttgcc ttatgaggaa
     1381 cattttcagg attgctcaca gcgtgggtta ggctgtcttc cttgctccat ccacacccct
     1441 gtatcgacta ctcgaagccg gtggcgagag ccgggacact tcgttcaact cgccccgccc
     1501 gatcaaaagc cggcgttgtt ggccactgcg ggccaatcgg ttgtggacct gggacatcga
     1561 caaactgcgt cgggcggtga aatgaaccgt caataggtcg gcgccctcgc ggcacgcaat
     1621 cgttcaactc acgacgaagc ggctactggc caagcgcctc atcgatcaca gcctgacgga
     1681 gcaaaacata gtgcccggcc aactcaccat ccatgccgat agcggctcat agatgaatcc
     1741 aaacccatgg ccttcttgcg gccggcgatt tgggagtgct gaagacccac aaagccggcc
     1801 cgtgtttatg atgacaatcc gttttcggaa gccgttttcg gaaaaccaat ttcgcattcg
     1861 caaatatcgc cccggttccc aggccatcga agatgctcgt ggcttctgtg cgaatcgtaa
     1921 gcgcaaataa aatagggcgg cattcgcgat tggccgcgag tgctgcccct attcaataaa
     1981 gcgataaatt tcgcgaagag tcgcggctga tcgttagccc cgttggcctt tgcgccagcg
     2041 gcggaatcca ccgagcccga gtaagccggt agccagcagc agcatcgatg cgggttccgg
     2101 agtcgccgac gtcccaccat tcgggtccac cgagtaatcg acgcttcccg aataatccag
     2161 tccataggag ctgcccgcgc gctgggcgac cgcgaacgac aacgtgttcc aacccgccgt
     2221 caggccgctg gtcaaatcga tgtggccttc ggttgaggtc aggcaaccga ttccaaaatc
     2281 cgagcaggtc ccatacccgt tccccgtgga tgtggcttcg ttaaatagcg tcactccgtt
     2341 caacacaacc gcagtcgaat cgtccgcgag gacattgacg ctgccgcccg ctggagtgcc
     2401 gccaataaag aacatatccg tgaacgtcac caccgttcca ttcgcaggcg cgaagtaccc
     2461 cggcgcgctg gggtcgcccg tctcgccgta cgacacccag gacgagctgc cgagcgggtt
     2521 ggcccagcct ggatgctttg gaatgttgag ggtagccgaa ctggagtcgt tagtagtctc
     2581 gctggcgcta ctgttcagcg agatcgtgtc ggctaaacta cagattgcaa aggtagataa
     2641 cacccccgcg aggaataacg tttttgtgaa ttgcagccta accttcatgc tggcagcatg
     2701 acatcataaa ttgtagaaaa caataggact acagtcccgg ttttgtgtac aaccactgaa
     2761 tcacccagaa ctcggcgctt tcttcgccac atggcttggt gcaagtacta aatgcttatg
     2821 gttcttgtac ttaccgtcta aaatacctct ctcgcggttt tggaatagta cgatcaactt
     2881 gtggtacaaa agcctctttt tatgccaaaa atggcgccca aagtgccctt tttacggctt
     2941 atatgagtct cgtaaaatcc gcgccccggc gttcctggcc gaaccaggcc atcaccgccg
     3001 cgaatacaaa aaccagaccg gcggtcaaag ccatggccga agcatagctg attttctgag
     3061 caaggatcgc ctcaagccag gcgacggacg ctgccagaag gacgccgcat tggtaggcga
     3121 atccgggcag aaaaccccgg acttcgttcg gcgaaagttc ggccagatgc gcgggaatca
     3181 cgccccaggc cccctgcacg aagaattgca gcaggaacgc gccggcggcg agcaacacaa
     3241 gggtggatga atacgcccac aagggaacaa cgagaattgc cagaatcatg gccagggaaa
     3301 tggatcgccg ccggccaaaa cggtcggaca gccacccgaa gccaatgccg ccgataatcg
     3361 cgccgatcat cgagatgccg gaaagaaccg cgcggactcg cgccgaaaac ccgaactggc
     3421 gttgcaggaa tgtcggatac atgtcctggg tgccgtgaga ggcgcagttc atggccatca
     3481 tgaaaatagt gagatagacg aacagtttcc agtggctgag aatcgtcttc gtcaaatcgc
     3541 cccagttcag actccgggac tcccgccaga cctcggattc cttcactcgg acgcggacaa
     3601 acagagccag cagggcaggc aggccgccaa tgaaaaacag aggccgccat ccccagcgcg
     3661 ggaaaacgat aaagaaacag atcgccgcga gcagatttcc gacggcatac ccctgctgga
     3721 ggaaaccgga aatcaagccg cgcaggcgcg gcggcacttt ttccatcgcc agcgacgcgc
     3781 cgacgcccca ttcgccgccc atgccgatgc cgaagagagc gcgcaggatc agaaacatcg
     3841 tatagctgct tgcgaagcct gtgaggactt cgacgatcga gtagaatacc aggtccagca
     3901 tgagcggcaa tcgccggccg tagcggtccg ccagcattcc gaataaaaac gcgccaacgg
     3961 gccggaatgc gagagtcaga gtgattgaga aaacaatatc ggagtcgctt ttgtgaaaat
     4021 cccggccaat tgcggtgaga caaaagacaa cgaggaaaaa atcgaacgca tcgagggtcc
     4081 agccgagaaa acaggcggcg acggcggctt ggctatccgc gcgggaaggc gccgaatcgg
     4141 atcgcattgg ggcacgatat catcatcggg cgcagaaaga tcgcggggcg gcattagaag
     4201 tctgcctg
//
LOCUS       DATNDT010000450         5643 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_409704, whole genome shotgun sequence.
ACCESSION   DATNDT010000450 DATNDT010000000
VERSION     DATNDT010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5643
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_409704"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="VN788_14670"
     CDS             <1..1048
                     /locus_tag="VN788_14670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21823.1"
                     /translation="VGGDQHVLALGSAITEIHVDPKLHTVAWDALGLIRDDDGAEGYN
                     FTYQFTVVAWNAVNINAFVDQGAINSRTIDCAQNGGQLPENYFYASNSGTSSALSCFF
                     SFLQNSAFASSKSIAVLPRGFGLVWNDGDHHIFQLAYNMDHSEILADRRNYNKKNQQI
                     AAPLPNPPSTHVASGFVSWSPYAIFEDNDTRRDYTFAEIVSGLGGSDVGLLQPPFSML
                     PNAADTGFLGLYSTCGEQGAEPTTTEEHTIENIPYQFAVPMLTGWNMQYVCSDQHVKD
                     VGIWIDDWSYQPPSGGNGGILHYKLSSILADDDSTRAHAVDHRITVLGLRGLTGGNVK
                     GVKPTVPKSAGNHQ"
     gene            complement(1123..2019)
                     /locus_tag="VN788_14675"
     CDS             complement(1123..2019)
                     /locus_tag="VN788_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007172036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HXU21824.1"
                     /translation="MGKETSAGQPGSGIAGKAGGFLIGGDLRVTRLGFGSMRLTGKGI
                     WGEPADRPEALRVLRRAVELGINFIDTADSYGPNVGEEIIAEALHPYPAGLVIATKAG
                     FDRTGPGKWVENGRPEHLRSACEGSLRRLRLDRIDLYQLHRIDTKVPAEDQFGALKDL
                     QAQGKIRHIGLSEVSVKQIQHAQKIVPIVSVQNRYSVTDRGSEDVLEYCEQQKLGFIP
                     WFPLAAGKLSGADSPINRVATQLKATPSQVALAWLLSRSPVMLPIPGTSRVDHLEENV
                     AAAGLPIDASQLKEIGRAAGAN"
     gene            complement(2344..2577)
                     /locus_tag="VN788_14680"
     CDS             complement(2344..2577)
                     /locus_tag="VN788_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007137833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HXU21825.1"
                     /translation="MAATTITVRTDGSIRIEGDFEIRDQDGKAFGLAGRTKIALCRCG
                     HSETKPFCDGSHKKVGFQSPIQAYDLPPVVTKP"
     gene            complement(2771..3949)
                     /gene="moeB"
                     /locus_tag="VN788_14685"
     CDS             complement(2771..3949)
                     /gene="moeB"
                     /locus_tag="VN788_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HXU21826.1"
                     /translation="MATKTQVPTAQSAGLSKQEVLRYSRHLIMPEVGMDGQLKLKKAK
                     VLCIGTGGLGAPLGLYLAAAGVGRIGLVDFDSVDFTNLQRQVLFGTSDVGRPKITAAA
                     DRLRNLNPEIQIDAYEAHLSSENALDLFKDYDIIVDGTDNFPTRYLVNDACVILGKPN
                     VYGSIFRFEGQITVFGYPEGPCYRCLYPEPPPPGLVPSCAEGGVLGVLPGIVGTIQAA
                     ETLKLIIGKGQPLVGRLLLFDALAMKFRELKLRKNPECPVCGKNPTVTKLIDYVQFCG
                     VRGEEAPSTVTNIPEITPRDLKSRMDKGDDLYILDVREPHEYQICNLNGHLIPLGELP
                     RRVHELDSSREIVAHCRSGKRSAEAVEFLQKAGFRKIWNLKGGILAWSDDVDSSVPKY
                     "
     gene            complement(4151..4435)
                     /locus_tag="VN788_14690"
     CDS             complement(4151..4435)
                     /locus_tag="VN788_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011143402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HXU21827.1"
                     /translation="MAVNVIIPTPLRAYTGRKESVEVQAATVAEALSALTSQYGELKK
                     HLFADDGRLRSFVNVYVNDEDIRYLQKDQTSLREGDTVSIVPSIAGGALV"
     gene            complement(4457..4909)
                     /locus_tag="VN788_14695"
     CDS             complement(4457..4909)
                     /locus_tag="VN788_14695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015706613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HXU21828.1"
                     /translation="MSRTALKVHGEIMKRIHAHGAETYPHECCGALLGRDGDESREVL
                     DLVPLANRRDDSPRNRFEVTADDVRLAEKTAREKRIELIGWYHSHPDTPARPSEFDRE
                     HAWPWYSYIIVSVQKGEARDTTSWRLHDDRAAYDPEEIENVAHPASIK"
     gene            complement(4906..>5643)
                     /locus_tag="VN788_14700"
     CDS             complement(4906..>5643)
                     /locus_tag="VN788_14700"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HXU21829.1"
                     /translation="ALRAGKIILDATSGNTGIAYAMVGAALGYRVKLCLPTSASPERK
                     QILAAYGVEIVFTPGDEGTDGAIRRVREIYNANPEKYFYPDQYGNPANPAAHYSTTAP
                     EIWEQTKGQITHFVAGLGTSGTFVGTTRRLKELNPKIRCISMQPDSGFHGLEGLKHMA
                     TAIVPAIYDASLADENMEVRTEDAQRVAKKLAREEGILVGVSAGGALWACLDVARRLP
                     PQEPALIVTVFPDSGEKYLSERFWSEE"
BASE COUNT         1140 a         1741 c         1469 g         1293 t
ORIGIN      
        1 tgttggaggc gatcagcatg tcctggcgtt ggggagcgcg atcaccgaga ttcatgtgga
       61 tccgaagctt cacacggtcg catgggatgc tttgggattg ataagggacg acgacggcgc
      121 cgaaggatat aacttcacct accaatttac ggttgtcgcg tggaacgcgg tcaacatcaa
      181 tgcgtttgtg gatcagggcg caatcaattc tcgaactatc gattgcgctc agaacggcgg
      241 ccagctaccc gaaaattact tttacgcgag taactctgga accagcagtg ccctctcgtg
      301 cttcttttct ttcctgcaaa acagcgcgtt tgcctccagc aagtctattg cggtcttacc
      361 gaggggtttc gggttggttt ggaacgatgg cgaccatcac atttttcaac tggcctacaa
      421 catggatcat tccgaaattc tggcggatag gaggaactac aataagaaga atcagcagat
      481 agcggcgccg ttgcccaatc ccccttcaac ccatgtggct tcgggctttg tgagttggag
      541 tccctacgcc atcttcgagg acaacgacac gcgaagggac tacaccttcg cggaaatagt
      601 ttcgggtcta ggcggcagcg acgtgggcct ccttcagcct cctttttcaa tgcttccaaa
      661 cgccgccgat acgggttttc tgggccttta ttccacctgc ggcgagcagg gtgctgagcc
      721 taccaccacc gaggagcata caatcgagaa tattccttac caattcgcgg ttcccatgct
      781 caccggatgg aatatgcagt atgtctgtag cgatcaacac gtaaaagatg ttggtatatg
      841 gatcgatgat tggagttacc aaccacccag cggcggtaac ggaggcatct tgcactataa
      901 gctctcctcc atcctggcgg atgacgacag tacccgcgcg cacgcagtcg atcatcgaat
      961 caccgttctt gggctgcggg gcctaactgg cgggaacgtg aagggcgtca aaccgacggt
     1021 ccccaagtcc gccgggaacc atcaatagct gctcgcagct ttcgcgggaa ggaagatcgc
     1081 acgtcgggcg gcggacctca acattccttc gcttcgagtc gtctagttgg ctcctgctgc
     1141 tcgcccaatc tccttgagct ggctcgcatc tatcggcaaa ccggctgcgg cgacattttc
     1201 ctcgaggtga tcaactcgcg aagttcccgg aatcggcagc atcacgggag atcgcgagag
     1261 aagccacgcc agcgcgacct gcgaaggcgt cgctttcagt tgcgtcgcga cgcgattgat
     1321 cggactatcc gcgccggaga gtttgccggc cgcgagtgga aaccaaggaa tgaatccgag
     1381 cttctgctgc tcgcagtatt ccaaaacatc ttccgaaccg cggtctgtaa cgctataccg
     1441 attctggacg ctgacgatgg gaacaatttt ctgcgcatgc tgaatctgct tcacgctcac
     1501 ttcggataag ccgatgtgcc tgatctttcc ctgagcttgc aaatccttca atgcgccgaa
     1561 ctgatcttcc gccggcacct tcgtatcgat tcgatgaagt tgatagagat cgatccggtc
     1621 caggcgcagc cgtcgcaggc ttccttcgca ggcggatcgc agatgttccg gccttccatt
     1681 ctcgacccac ttgccgggtc ccgtccggtc gaagccagcc ttcgtcgcaa tcactaggcc
     1741 agccggatag ggatgcaaag cctccgcaat aatttcttcg cccacgttcg ggccgtagga
     1801 gtccgcagtg tcgatgaaat taattcccaa ctcgaccgca cgccgcagaa ccctcagagc
     1861 ctcgggccga tccgcgggct cgccccaaat tcccttgccg gtaagccgca tcgaaccaaa
     1921 tccaaggcgc gtcactcgca gatcgccgcc gataagaaat ccgccggcct ttccggcaat
     1981 ccccgatcct ggttgccctg ccgaggtttc ctttcccatt tttcgtttgg tcctttccgt
     2041 attgcagcta tgaatggggg tgaaacaacc ccgcgaaaat ccacaccgga ggtttatgta
     2101 gcgccatccc ttcagggtgg cattttgctc gtcgcgcatt cgcaaactgg gcaccaacat
     2161 cgtgccggaa ccaccacaat gtcatcctga gcgaagcctc gcgctttttt tcttcgcgct
     2221 gtgtatgcgc ggcgcgaggc gcggtcgaag gatctccctt cgatgtaacc acaaccgccg
     2281 cgaaagcaaa gagcgtggcc gtcttttccg gatttattgc tttgacggag atttctcggt
     2341 agcttacggc ttggtaacca caggcgggag atcgtaagcc tgtatcggac tctggaatcc
     2401 aaccttcttg tgcgagccat cgcaaaatgg cttggtttcg gaatgcccgc agcggcaaag
     2461 tgcaatcttg gtgcggcccg ccaaaccaaa cgccttgcca tcctggtcgc gtatttcgaa
     2521 gtctccttct atccgaatcg aaccgtcagt gcgcactgta attgtcgtcg ccgccattca
     2581 atcctccaat tcctctcgct ctcagtttaa caggcatcgc gcttttgacg tcatcctgct
     2641 tgccctgacg aaggaaggga gcgaagcctc gcgctttttt gcttctcgcg cggtctatgc
     2701 gcggcgcgag gcgcagtcga aggacctctc ttcgatgtgg ccaaacctcg aagaaaacca
     2761 aagaaaccgc tcagtatttc ggcacgctcg aatccacatc atccgaccaa gccagaatac
     2821 cgcccttcaa gttccatatc ttccggaagc ccgccttctg caaaaactcc acggcctccg
     2881 cggaacgttt tccgctgcgg caatgcgcca caatctcccg cgagctatcg agttcgtgaa
     2941 cgcgccgcgg aagctctccc agtggaatca agtgcccgtt caaattgcaa atttgatact
     3001 cgtgtggctc gcgcacgtcg agaatataca agtcgtcgcc cttatccatc cgcgatttca
     3061 gatcgcgcgg agtaatctca ggaatatttg tcacggtgct aggagcctcc tcgccgcgaa
     3121 cgccgcagaa ttggacgtag tcgatcaact tcgtcaccgt cggattcttg ccgcaaacag
     3181 ggcattcggg atttttccgc aatttcaatt cgcggaactt cattgccaac gcgtcaaaaa
     3241 gtagcagccg cccaacaagt ggctgccctt ttccgataat tagcttcagc gtctcggccg
     3301 cttgaatcgt gccgacgatt cccggcagca ctccaagtac gccgccctcc gcgcacgaag
     3361 gcaccaagcc cggcggaggc ggttcgggat acaagcagcg gtagcacgga ccttccggat
     3421 agccgaacac cgtgatctgt ccttcgaacc ggaaaatcga gccataaaca ttcggcttac
     3481 cgagaatcac gcaagcatcg ttcaccaggt agcgggtcgg aaaattatcc gtgccgtcca
     3541 cgatgatgtc gtaatccttg aaaagatcga gcgcattctc gctgctcaga tgcgcttcat
     3601 acgcgtcgat ctgaatttcc ggattcagat ttcgcaggcg gtcggcggct gcagtaatct
     3661 tcggccggcc cacatcgctc gttccgaaaa gaacctgccg ttgcaggttc gtgaaatcca
     3721 cggaatcaaa atccaccaag ccgatgcggc ccactcccgc tgccgccaaa taaagtccca
     3781 gcggcgcgcc cagcccgccc gttccgatgc aaagcacctt ggccttcttc agcttcagct
     3841 gaccgtccat gcccacttcc ggcatgatca ggtgccggct gtagcgcagc acctcctgtt
     3901 tggaaagccc cgcgctctgt gcggttggaa cttgcgtttt cgtcgccata aaaactctcc
     3961 ttacctgtag cgccaccctt taaggctggc atctcgcttt tgacgtcatc ctgcttgccc
     4021 tgacgaagga agggagcgaa gtcccgcgtt ttttccttcg cgcggttttt gcgcggtgcg
     4081 ctcagcaggg ctcccgggtc gacggttagt cgagcccggg agatcagtcg tcgaaggacc
     4141 tctcttcctt ctagactagc gctccaccgg caatcgacgg cacgatgctc actgtatcgc
     4201 cctcgcgaag gctggtttga tccttctgca ggtagcggat gtcttcgtca tttacatata
     4261 cgttcacaaa actccgcaag cgcccgtcgt ccgcgaagag gtgcttcttc aattctccat
     4321 actggctggt cagcgccgaa agcgcctccg caaccgtggc cgcctgcact tccacgcttt
     4381 ctttcctgcc cgtgtaagct cgcagcggcg tcgggataat cacattcact gccattctca
     4441 gtcctcctcg tgttgactat ttgatgctcg cgggatgcgc gacgttttcg atctcctctg
     4501 ggtcatacgc cgcccggtcg tcatgcagcc gccacgacgt tgtatctcgc gcctcgccct
     4561 tttgcacgct cacgatgatg tagctgtacc acggccaggc gtgctcgcga tcaaactcac
     4621 tcggccgcgc gggcgtatcg ggatgggaat gataccaccc gatcagctcg atgcgcttct
     4681 cgcgtgcggt tttttccgcc aagcggacgt catccgcagt tacttcaaac cggtttcgcg
     4741 gcgaatcgtc tcgccggttt gcgagcggca ccaggtcgag cacctcgcgc gattcgtcgc
     4801 catcgcggcc cagcagcgcc ccgcaacact catgcggata cgtttccgcc ccgtgcgcgt
     4861 ggatgcgctt cataatttcg ccgtgaactt tcagtgcagt gcggctcact cttcgctcca
     4921 gaatcgttcg ctcaaatatt tttctccgga atcggggaac accgtcacga tcaacgcggg
     4981 ctcctgcggc ggcaagcgtc gcgcgacatc caggcacgcc cataatgcgc ctccggcgct
     5041 gacgcccacc agaattcctt cttcgcgcgc aagttttttc gccacgcgct gcgcatcttc
     5101 ggtccgcact tccatatttt cgtcggccag gcttgcgtcg taaatcgcag gcacaatcgc
     5161 ggtggccatg tgtttcagac cttcgagccc gtgaaaaccc gagtccggtt gcatgctgat
     5221 gcagcgaatt tttggattca gttctttgag ccgccgcgtg gttccgacaa acgtcccgct
     5281 ggttcccagt cccgcgacga aatgcgtaat ctgtcctttc gtttgctccc aaatttctgg
     5341 cgcggtcgtc gaataatgcg cggcgggatt cgcgggattg ccgtactggt cgggataaaa
     5401 atatttctcc ggattcgcgt tataaatttc gcggacgcga cggatcgctc cgtccgttcc
     5461 ctcatcgccg ggagtaaaaa caatttcaac gccatacgcc gccaaaatct gtttgcgttc
     5521 cggcgacgcg ctggtaggca agcaaagttt cacgcggtat cccagcgccg cgccgaccat
     5581 tgcatacgcg attcccgtgt ttccgctggt cgcgtccaaa attatttttc cagcgcgcaa
     5641 agc
//
LOCUS       DATNDT010000451         2806 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3557075, whole genome shotgun sequence.
ACCESSION   DATNDT010000451 DATNDT010000000
VERSION     DATNDT010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3557075"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..507)
                     /locus_tag="VN788_14705"
     CDS             complement(64..507)
                     /locus_tag="VN788_14705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014747681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXU21830.1"
                     /translation="MKVSEVMCRDARLIDANDTVQHAAEIMAQEGLGFLPVQENDRLV
                     GMLTDRDIVIRCLAQGKDGKARVRDAMTRDVKYCFERDDLEDVMENMAEIQVRRLPVL
                     NAEKRLIGIISLADAARMHSPDTVGIAFSGVVTPGGYHAGDAGRA"
     gene            complement(604..1338)
                     /locus_tag="VN788_14710"
     CDS             complement(604..1338)
                     /locus_tag="VN788_14710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21831.1"
                     /translation="MTQSAPTFRDARIQSLWNDVWQTFQNGGVYEPIIAIVEAELAGF
                     GAALPPNDAAAVIDFQKAQNTHLFQSRHFANELAKITVFQEEVRWALQQTMAEMDKYN
                     QHTLRSLTLAHGGIVIATLAYIQSLHQPPLAFVAVVGLGAFGYLLTIIGAAISVRLMA
                     PLLKLLGELLAPRLAVTERQSRIEGFQKGSKKVAKWTRPFFYASAGCLIFALFIGIKA
                     LVDERNSIGPPSLAAHSAAIPFMRQS"
     gene            complement(1335..>2806)
                     /locus_tag="VN788_14715"
     CDS             complement(1335..>2806)
                     /locus_tag="VN788_14715"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HXU21832.1"
                     /translation="FQHGEVYVTDDGAETDLDLGHYERFTGVSSAQSDNVTAGRIYQT
                     VISKERRGGYLGRTVQVIPHVTDAIKEFVMAGTDDLDFLLCEIGGTVGDIEGLPFFEA
                     IRQLGNDLGRDQAAFVHLTLVPYIEAAGELKTKPTQHSVKELRAIGIQPDILLCRASH
                     PVPDDERRKIALFCNVRPECVIPALDMPTIYDAPVRYHDAGFDTQVLNVFGIKDAPQP
                     DLSKWKEVVERVTNPEGEVRIAVVGKYIQVKDSYKSLSEALTHGGLANNVKVHMDWID
                     SEIFERDDAASFLENVHGILVPGGFGERGTEGKIQAVKFARERNVPFFGICYGLHMAV
                     IESARDLAGLEGAGTTEIGQPTHPVIGLMTEWTKGNVKETRSAEDDLGGTMRLGAYPA
                     VLTPGSRVAEVYGTTEISERHRHRYEVNDEYTDQLAKHGLKVSGRSPDGRLPEIMEIP
                     EHPWFIGVQFHPELKSRPLAPHPLFTSFIAAAVRQSRLV"
BASE COUNT          564 a          808 c          817 g          617 t
ORIGIN      
        1 ctatctgctg gtgatcgaac gcgcacccaa ggccgtacaa aaagcactta aggcggcttg
       61 atatcaggcc ctgcccgcgt cgcctgcgtg atagccgccc ggcgttacca cgcctgagaa
      121 cgcgatgccg accgtgtccg gggaatgcat ccgtgcggcg tcggcgagcg agatgatgcc
      181 gatgaggcgt ttttccgcgt tcaggaccgg gagacggcgg acctgaattt ccgccatgtt
      241 ctccatgacg tcctccagat cgtcgcgctc gaaacaatat ttcacgtcgc gggtcatggc
      301 gtcgcgcacg cgagctttgc cgtctttccc ctgagcgagg caccggatga cgatgtccct
      361 gtcggtcagc ataccgacaa ggcggtcatt ttcctgaacc ggcaggaaac ccaagccttc
      421 ctgcgccatg atttcggcgg cgtgctggac ggtgtcgttc gcgtcgatca ggcgggcgtc
      481 acggcacatc acttcggaaa ctttcatggc cgactccttt gctagctttc gagagaagcc
      541 gcggctcctc cgagcgctaa caaagtactc cgttccccgt tccaggagat taccctcaaa
      601 tcgctaactt tgccgcataa acggaatagc tgcggaatgt gcagcgagcg aaggaggacc
      661 gatagagttc cgctcgtcca cgagtgcttt aatgccaata aatagcgcaa atatcaggca
      721 accagcggat gcatagaaaa aaggtctcgt ccattttgct acctttttcg aacctttttg
      781 aaatccctca atccgcgact gacgttcggt tacggctaat ctcggggcga gcaattcccc
      841 caataatttc aaaagtggcg ccatcaatcg tacgctgatt gctgcgccta tgatcgttag
      901 taaatagcca aatgcaccca atcccacaac tgctacaaat gcgagcggtg gctgatgaag
      961 agattgaata taagctaaag ttgctatcac gataccaccg tgagccaaag tgagagagcg
     1021 gagcgtgtgc tgattatact tatccatttc tgccatcgtt tgctgcaggg cccagcgaac
     1081 ttcttcttga aaaactgtta ttttcgcaag ctcgtttgca aaatgcctgc tttgaaacaa
     1141 atgagtattt tgcgctttct gaaaatcgat gacagctgca gcgtcattgg gtggcagggc
     1201 tgcgccgaaa ccggccagct ccgcttcaac aattgctata atgggttcgt atacaccccc
     1261 attttgaaaa gtttgccata cgtcattcca gagcgattgt attcgcgcgt cacggaacgt
     1321 gggcgcggac tgcgtcatac caaccggctc tggcgcaccg cggccgcgat gaacgacgtg
     1381 aacagcgggt gcggtgcgag cgggcgggat ttcaattccg ggtggaattg gacgccgatg
     1441 aaccaggggt gttcggggat ttccatgatc tccggcaggc ggccgtcggg cgaccggccg
     1501 gacactttca acccatgctt tgcgagttgg tcggtgtatt catcgttcac ctcgtaacgg
     1561 tgacggtggc gttcactgat ttccgtcgtg ccgtagactt ctgcgacgcg gctcccgggc
     1621 gtcagcacgg cgggataggc accgaggcgc atggtgccac cgaggtcgtc ttcggcgctg
     1681 cgggtttcct tgacgttgcc cttggtccac tcggtcatca ggccaatcac cggatgtgtg
     1741 ggctgaccga tttccgtggt gcctgcgcct tccaagcctg cgagatcacg cgcggattcg
     1801 atcaccgcca tgtgcagccc atagcagatg ccgaagaacg gcacgttgcg ctcgcgggcg
     1861 aatttgacgg cctggatctt tccttcggtg ccgcgttcgc cgaaaccgcc gggaacgagg
     1921 atgccgtgca cgttttcgag gaacgatgcc gcgtcgtcgc gttcgaatat ctcgctgtcg
     1981 atccagtcca tgtggacctt cacgttgttg gcgaggccgc cgtgagtgag cgcttccgac
     2041 aatgatttgt aggaatcctt cacctgaatg tacttgccga ccacggcgat gcgcacttcg
     2101 ccttcgggat tggtcacgcg ctcgaccact tccttccatt tggaaagatc gggctgcggc
     2161 gcatccttga taccgaagac attaaggacc tgcgtgtcga aaccggcatc gtgatagcgc
     2221 accggcgcgt catagatcgt cggcatgtcg agcgctggga tgacgcattc cggccgcaca
     2281 ttgcagaaca gcgcgatctt gcggcgttcg tcatccggca ccggatggct ggcgcggcac
     2341 aaaaggatat cgggctggat gccgatggcg cgcagttcct tcaccgagtg ctgggtcggc
     2401 ttggttttca actcgcctgc ggcttcgatg taaggaacca gcgtcagatg cacgaaggcc
     2461 gcctgatcgc gcccgagatc gttgccgagc tggcgaatcg cttcgaaaaa cggcaggcct
     2521 tcgatgtcgc ccaccgtgcc gccgatttcg cagagcagga aatcgagatc gtcggttccc
     2581 gccatcacga attccttgat ggcgtcggta acgtgcggga tgacctgcac cgtgcggccg
     2641 agatagccgc cacggcgctc tttcgagatc accgtctggt agatgcgccc ggcggtcacg
     2701 ttgtcggact gggccgagga aacgcctgtg aagcgttcgt agtggccgag atcgagatcg
     2761 gtctccgccc cgtcatctgt gacgtagacc tcgccatgct gaaacg
//
LOCUS       DATNDT010000452        15773 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3568958, whole genome shotgun sequence.
ACCESSION   DATNDT010000452 DATNDT010000000
VERSION     DATNDT010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 15773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15773
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3568958"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="VN788_14720"
     CDS             complement(<1..303)
                     /locus_tag="VN788_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21833.1"
                     /translation="MKRSSDGNAMDSRHKFGLRPRPILGLGSPPVDPASAKPQPARAG
                     RPSVMQAPVLVLNATFEPINVTAVRRALILMLKGVAQAEETSHTEVHSTSKAIQVPS"
     gene            complement(300..518)
                     /locus_tag="VN788_14725"
     CDS             complement(300..518)
                     /locus_tag="VN788_14725"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HXU21834.1"
                     /translation="MRVLLVDDVLTTGATLDAGARVLKKAGAATVLGLTVGRVRSGLP
                     PPASVLKRKKPNGKPEGSVEHLYYQGTV"
     gene            complement(523..1134)
                     /locus_tag="VN788_14730"
     CDS             complement(523..1134)
                     /locus_tag="VN788_14730"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HXU21835.1"
                     /translation="MILKDALGALASVVFASPCRICGEALTNASRIPICERCLDGFEK
                     IIEPMCAGCGRPFAMPRAGAIESSLCRLCRVGFYGFDRARSFAIYDEALTEAVLLLKY
                     QELTRLGDWFAERLFEVVSRVPAEWRADLVVPVPLHADRRRERGYNQAELIARPLAER
                     LGIPFNSQILVRTKPRPAQTSALSDGTLEIGAWRVRYQASSEC"
     gene            complement(1266..1619)
                     /locus_tag="VN788_14735"
     CDS             complement(1266..1619)
                     /locus_tag="VN788_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HXU21836.1"
                     /translation="MDKKRVDYYKKKLAARREELLRTIARTQQEGREADEDPTVDLAD
                     KAANSYTKEFLFGQTHNERSLLQLVDDALSRIRENSFGDCVSCHQELQQKRLEAVPWT
                     RYCISCQEKKENGQL"
     gene            complement(1798..2751)
                     /locus_tag="VN788_14740"
     CDS             complement(1798..2751)
                     /locus_tag="VN788_14740"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HXU21837.1"
                     /translation="MASVPPEMSSSPLPPAHGGPPQSATHRIFLGSDGIRAGWRLAIA
                     IAIFLALLTVVSAALVRVPHVRAWAVSGGPGALFFGEGILALALVLTVWVMARIEDRS
                     WADYGLPSSAAFGKRFWQGAPYGFAMLSLLMGLMAASHAFSFGGRALTGAGAVRYGLL
                     YLAAFTLVAFFEEFSFRGYMQATLASGIGFWPGAVLLAVVFGALHLGNKGEAKFGAVM
                     AGAFGLLAAFTLWRTGNLWFAIGMHGAWDWGESFFYGVPDSGVVLKGHLMNASFHGPA
                     WLNGGTVGPEGSVLVAAVLVLAAIGIHYIFPKRPTPQDQLA"
     gene            3015..4412
                     /gene="lpdA"
                     /locus_tag="VN788_14745"
     CDS             3015..4412
                     /gene="lpdA"
                     /locus_tag="VN788_14745"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HXU21838.1"
                     /translation="MAKYDLVIIGSGPGGYVAAIRASQWGLKTAVVEKDPFLGGTCLH
                     VGCIPTKVFLHHADIYDNFQRAEEFGFEVKDVKINWPAMLARKDKIVKKHAGGIGMLF
                     KKNKVDSITGWGRIAGPGRVSVEKDGQTTELETTYTMIATGSEARSLPGVEIDGKTTL
                     TNREILQLQAIPKSLVVVGCGAVGVEFASIYRTFGADVTLLEALPRIVPLEDEEISPE
                     LEKAFKKKGIHIETEAKVESVKKDAKGATVTFNDKTGKSQSVSAEKVLIAVGRRPLTE
                     NIGLEKTKVKLERGFIHANAYLETDEPHLYAIGDIVAGLPQLAHAASMEGIIAVGRMA
                     GEEVQPFDRKRCPNATYCEPQIGSVGLTEKQARDAGYEVKTGKFPFLGNSKASILGHH
                     EGFIKVVAEAKYGEILGVHAIGPLATEIIAEPVTALGLEATLDDMAAQIHAHPTVWEA
                     LGDAFNSVRGLTINY"
     gene            4526..5269
                     /gene="lipB"
                     /locus_tag="VN788_14750"
     CDS             4526..5269
                     /gene="lipB"
                     /locus_tag="VN788_14750"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HXU21839.1"
                     /translation="MTQGEKICWLVDLGLIPYGAACELQRRLIEARKARAIPDVLLLC
                     EHPHVVTLGRNAKREHLLASKQLLTEMNVELRSTDRGGDITYHGPGQIVGYPILDLTK
                     HRRDVRWYVEQLEEAMIRTTADFGIIARRIEGQHGVWIDGPSGEEKLGALGVHLSHWV
                     TSHGFAYNVSTDLRYFDLIVPCGIADKRSTSLEHALSRDVSIEEARARVALHFSQVFD
                     RELVAVVPRSLEETLRAALNSQPIESTLV"
     gene            5425..6792
                     /locus_tag="VN788_14755"
     CDS             5425..6792
                     /locus_tag="VN788_14755"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HXU21840.1"
                     /translation="MTQSSVVESGPRLGDPTAKEHMSSKLTNDQYLDLYYFMLLNRRL
                     EERLVRLFRQNKIVGGVYSSLGQEAVSIGTAYALEPRDWLAPMIRNIGSLLVKGFKPR
                     DIFTQHMAKYTSPTQGKDGTSHFGDLKTRHVVSPISMLGDLIPVMTGVAMAGRYLGQN
                     IVAMTWIGDGGSSTGAFHEGLNLAAQQRAPFVLIIENNQWAYSTPVARQVPLKDLADR
                     AAAYGIAATSVDGNDVIAVYDAAKSAVDQCRAGHGPCVIEVKTMRMKGHAQHDAADYV
                     PKAMMDYWKAQDPIARYENYLTQHHLLDSAKKSAIDARIERELDEDQKFAEESPMPPP
                     ELAEQGAYCEGCHTIQANWQRPKKEVTPPASSVEAIWKVADFGAFKRPAVPEAATEHE
                     NRDGNGANSFDELPSAETQIEKQARLRVPFGRGPKDRSVELERRDKVAERPPRHHKPH
                     KRRRR"
     gene            6792..7766
                     /locus_tag="VN788_14760"
     CDS             6792..7766
                     /locus_tag="VN788_14760"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HXU21841.1"
                     /translation="MPEITMLEAIRAALFEEMERDPAVVTLGEDIGVYGGAFKATEGL
                     LAKFGPERVMDTPISETAIVGAACGMSYLGLRPVVEMQFIDFIACCFNQVTNFVAKSH
                     YRWGAPVPIVVRGPSGGGVHGGPFHSANPEMYFVHTPGLKVVYPSTAYDAKGLLKSAI
                     RDNNPVLFFEHKFLYRRLKEELPSEDYTVPLGKAVVRREGKDLTILSYAAMMLPTLDA
                     AQQLSREGIEAEVIDLRTLLPLDREAILASVKKTNKLLIVHEDTRTGGIAGEIAAIVC
                     ESAFGDLDGPITRVTALDTPVPYAPSLEERFLPNAEKVAVAARELAKY"
     gene            7902..8153
                     /locus_tag="VN788_14765"
     CDS             7902..8153
                     /locus_tag="VN788_14765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21842.1"
                     /translation="MGKTKGLNKAEFEVGSEVRIADRAFLEEFLEAGQYHNELEPEQV
                     EYAGRVAKVKNIEFFHGGDEIYTLEGIPGVWHEECLSPA"
     gene            8462..9841
                     /gene="sucB"
                     /locus_tag="VN788_14770"
     CDS             8462..9841
                     /gene="sucB"
                     /locus_tag="VN788_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HXU21843.1"
                     /translation="MAVDVIMPQMGESIFEGTITKWLKKPGDKIERDEPLFEISTDKV
                     DAEIPSPSAGVLKEIKVNEGQTVPIQTVVALIEAAGATSQPAATSPPTAKPDAKSSSP
                     ASSSASTPPAPAAKPAAVKPGPAPAATPPVGSKVPAPVAAPSFSQPSRHNGGDKIRSS
                     PLVRRIARENQIDLTQVSGTGAGGRVSKRDILAAAEGGATTSAAPAPAPSGVPSHPAI
                     EVGVPRERIYFGHYEVQPMSNMRQKIAEHMVLSKRVSPHVYSVEEVDATGIAKLRANA
                     KEKFEKESGTKLTFMPFFIRAAVEALRAFPTVNSSVDGTNIVLHKECNIGIAVALDWG
                     LIVPVIKNAEEKNFFGLARAVTDLAERARTKKLKPEEVTESTFSITNPGVFGGLFGLP
                     VINQPNVAILGLGTIEKRPVVIDDAIAIRSMVYLTLSYDHRAVDGAIAHQFMGHVKHT
                     LQDWTESIF"
     gene            9851..11059
                     /locus_tag="VN788_14775"
     CDS             9851..11059
                     /locus_tag="VN788_14775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HXU21844.1"
                     /translation="MRSRRSLLHPTSAMSRSHALELWGVPPWKIEFTPPKRSLPSTVD
                     FAVVGAGFTGLAAAAWLRILAPGKSVAVLEAGRIGNGASRRTGGLVLSESAAGDLPGL
                     GDVLSGLKKILRKLRVESDLLLPGAWEIAHSDHAEPSDNARPLEGRSPIQWRDFDSRE
                     IRVAGTVPGGTLDPGKQVSGLARAAVSLGAVIAENCGIRKIEWTTPPTLHHTGGFLRA
                     KKILLATNALSLEISGLRKSVIQPKLTLAARTAPMSEKELGEMGLAGRKPFYTIDFPY
                     LWGRVCADRSIVWGAGLVDAPSLGELEDVDVSKGRAAKMFASFEKRVHGLHPALAKAR
                     FTHKWGGPIAFRENFNPVFAHHPESANGIVLGIFAGHGVALSVYLGTWAAEALLGNRA
                     LPVWGQLKSK"
     gene            11131..11730
                     /locus_tag="VN788_14780"
     CDS             11131..11730
                     /locus_tag="VN788_14780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21845.1"
                     /translation="MAKKRNNRAQPSLFPPKKTEVPSIPAEEALSFLRETRGVVTWTA
                     RDMADSLKISVADAKRVLPILELQGYVKPAGANEWMTTLAGEEVSGSKAPRFTRERVA
                     QALNDLHDRIAEINRNSKTPYKITEAVAFGDFLSDRPRVQAAEAGIGLERREIGADDA
                     GDGVQQRDFLRQLHAKGGVIQVRPYEPWMSERRHRSLLR"
     gene            complement(11818..12345)
                     /gene="bstA"
                     /locus_tag="VN788_14785"
     CDS             complement(11818..12345)
                     /gene="bstA"
                     /locus_tag="VN788_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016820583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HXU21846.1"
                     /translation="METDLRYPIGKFQPAENVTDIQRGALIAAIAETPERLKAAIARL
                     NEAQLDTPYRPGGWAVRQVVHHLPDSHMNAFVRFKLALTEDSPTIKVYDEARWAELAD
                     AKAPVEPSLLLLENLHKRWISLLNSLTVTDWQRKFHHPERGTVTLENNLQLYAWHGRH
                     HIAHITALRERNNWR"
     gene            complement(12504..>15773)
                     /locus_tag="VN788_14790"
     CDS             complement(12504..>15773)
                     /locus_tag="VN788_14790"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU21847.1"
                     /translation="TDSSGAALVSATVQARNVGTNITQSTVTDASGRYRIPDLPIGTY
                     ELQTSQTGFQTVVHKGITLTVGANLVVDFSLPVGQVSQTVNVEGEVSRVETQTASVSS
                     LVTSQQMSQLPLNGRNFEQLLSLAPGVLTVKQAFVTGGAGGISSGFYGPSDTYSVAGS
                     RPVGQVFLLDNQDLQGYWEKGAGASITGNSLGVDAIQEFQLLTNTYSAQFGGTGAAMN
                     AVSKSGTNDFHGSAYEYLRNSALDARNFFDGSKIPPFRRNQFGGSLGGPVKKDKAFFF
                     VNYEGLRSALGITGNQFVPDRDSLGLNTANSCPGAGGGGAEVPYQFGCVQVGAARTRV
                     AINPGVVPYLPGQLYPQVPSLTSPEQIVGAGPFRGLPSGNQLITEVSNEPTSENYLLG
                     RVDYTLGSKDSLFGRYISDTAYLLLPAPFSNLSNWPAAFHGGDQFFTLEEKHIISATS
                     INEVRFSFSRTNERSDQKFLSDPATDPLQFYLGALFNGSPAYSPGDREDGNIVVAGGS
                     SNVGPGPTARWHLIQNKFTGGDDFFWSHGAHSLKVGASVSRVQNNIAVGREGGLFVFG
                     SIAGFLASTPVQFQGETNPHPGFTRTRYSREIQFFPYIQDDWKIKSNLTINIGLRYDY
                     ETNPVCVTEPCNAILNPLSSPGFTQVHHVLKSNPDALNFDPRVGLAWDPFSDHKTSIR
                     AGFGIFHEPIAARTYLNAYSQNPPSQTVVLAGPPAVSLFPHPAACGPVGSACLNPPFG
                     TVYGLDYTSDSGPYEMQYNLTVQRDVGHGMVASLGYVGAQGVHLYNQRNQNSPSLVDA
                     AGNPVSPTCTSPSCFFKGPVPNPAFNGLDNTAPTSHSTYNGLVASLTRQVSRNLQGQV
                     SYTFSRCIDDGSASSGLEQASAEILDARNQGFDRGPCTFNVTHALRVNSIYTLPFTGN
                     RLVSGWQLGEVLSATTGYPVNVTNGLLPQVANTGGLTADRPNYSGAPGCHPSQITDTV
                     NAQQRTVQWFNPACYAVQPFATLGNVRRDSLIGPGLLDLDFSIIKQTRITEKLNTEFR
                     AEFFNIINHTNLGQPNGAVFSGAGGAGAAAGSFIAGNSGLITSTSTTSRQIQFALKLI
                     F"
BASE COUNT         3490 a         4511 c         4549 g         3223 t
ORIGIN      
        1 cgatggcact tggatggcct tggaagttga atggacttcc gtatggctcg tttcctccgc
       61 ttgtgctact cccttcagca ttaggattag cgcacggcga acggccgtca cgttgatcgg
      121 ctcaaatgtg gcgttcaaga cgagcactgg agcttgcatg accgaaggtc tgcccgcccg
      181 agcgggctgc ggcttcgccg acgcaggatc aaccggcggg ctgcccagcc ctaaaatggg
      241 ccgaggccgt agaccgaatt tgtggcggct gtccatcgca ttaccgtcac ttgaacgctt
      301 cacactgttc cttgatagta tagatgctcg accgacccct ctggtttgcc atttggtttc
      361 ttccgcttca aaacggatgc gggcggaggc aggcccgagc ggacgcgccc caccgtcaat
      421 cccagaacgg ttgcggcccc cgctttcttc agcactcgag cgcctgcatc cagagtggct
      481 ccggtagtca atacatcatc taccagcagg acgcgcacgt tgtcaacatt cgctgctggc
      541 ctgatagcgt acgcgccacg caccgatttc cagtgttccg tccgagagag cactagtttg
      601 ggccggccgc ggtttcgtgc ggaccaggat ttgggaatta aacgggatgc ccagccgctc
      661 ggccagcgga cgcgcaatta actccgcctg attgtagcca cgctcgcggc ggcggtccgc
      721 atgcagtggg acaggcacaa cgaggtcggc gcgccactcc gcaggcacgc gcgagaccac
      781 ctcaaataac cgttccgcga accaatcgcc caaccgcgtc agttcttgat atttgagcaa
      841 gagaacggct tcggtaaggg cctcgtcgta aatcgcgaag ctgcgcgcgc gatcaaagcc
      901 ataaaaacca acgcggcaca agcggcaaag tgagctttca atagccccgg cccgcggcat
      961 cgcgaacggg cgtccgcaac cggcgcacat cggctcgata attttctcaa agccgtccag
     1021 gcatctttcg cagatcggga tgcggctggc gttggtcagg gcttcgccgc aaatgcggca
     1081 cgggctggca aacaccaccg atgcaagtgc gccgagggcg tctttcagga tcacggatac
     1141 ccgtccataa tcgcgaacta tggccggaca ttactccaag tcctgaatga agaaatagag
     1201 atgatcgtct ctagagagtt gaaaattgcg aagtctgcca gacgggcaag aaccacaaaa
     1261 acctcttaca gctgcccgtt ctctttcttt tcttggcagc taatgcagta gcgggtccaa
     1321 ggcacggctt ccagacgttt ctgctgtagc tcctgatggc acgaaacgca gtcgccgaac
     1381 gaattctccc gaattcgcga cagcgcgtcg tctaccagtt gcagcaggga tcgttcgttg
     1441 tgtgtttggc cgaacagaaa ctccttggta taggaattcg cggccttgtc ggcaagatcc
     1501 acggtaggat cctcgtcggc ctcgcgtcct tcttgctgcg tgcgcgcgat cgtccgcagc
     1561 aactcctcgc ggcgcgccgc gagtttcttt ttgtaataat ccactcgctt cttatccatg
     1621 gtctgcactc tactgtggtc cagaatggct cacccaaatc gggcgattct aaacgtctga
     1681 accgcccttg tcaaacaagc atcgtcgcaa atccttaaat ccttcatccc ttggagtggt
     1741 tcatgcagct tccagtagat gcttcgtgcg cccaaaagga ttccacgctg cgttctctta
     1801 cgcgagctga tcttgcggtg tcggtcgctt tggaaaaatg tagtgaatac ctatcgcggc
     1861 cagcaccaag accgcggcca ccaaaacgct tccctccggt ccaaccgttc cgccattcag
     1921 ccacgccggc ccgtgaaacg atgcattcat cagatgacct ttcagcacca cgccgctatc
     1981 cggcacacca taaaaaaaag actcgcccca gtcccacgcg ccatgcatgc cgatcgcgaa
     2041 ccatagattt cctgtccgcc aaagtgtgaa cgccgccagc aggccaaacg ctccggccat
     2101 taccgctccg aatttagcct cgcctttgtt ccccaaatgc aaagcgccga acaccacggc
     2161 cagcaggacc gcccccggcc agaatccgat tcccgaggca agtgtcgcct gcatatatcc
     2221 acgaaaggag aattcttcga agaacgcaac cagagtgaat gcggccaagt agagcaggcc
     2281 gtagcgcacc gcgcctgctc cagtcaacgc ccgccctccg aatgaaaacg catggctggc
     2341 ggccataagt cccatcagta agctcagcat cgcgaatccg tacggcgcgc cctgccagaa
     2401 tcttttgcca aacgccgcgc ttgacggcag gccatagtct gcccacgacc gatcttcgat
     2461 tcgcgccatc acccataccg tgagcaccag cgccagcgcc aggatacctt cgccgaaaaa
     2521 cagggcccca ggaccaccgc tgacggccca agctcgcacg tgcggcacgc gtaccaaggc
     2581 ggcgctgacc accgtcaaaa gcgccagaaa tatcgcaatg gctattgcca gacgccaacc
     2641 cgcgcgaatt ccgtccgaac cgagaaatat gcggtgtgtg gcgctttgcg ggggcccgcc
     2701 gtgggccggg ggcaaaggac tcgaactcat ttcgggcgga acggaagcca tggccgacct
     2761 cccattaact ttaatcatgg cctgtgaagc gccaccaaaa tacgccgcat caccttgcgc
     2821 accaagcgtt tctcgtagca gattgttcgc gccgcgtgct cgcgcgaagg accgccccgg
     2881 ttgcggtggc tattccccgc tgctaaaatc ttgagtctgc tgcgcctgga cgatctgccg
     2941 tcatcaagcg tccttccaag caggatgagt gcccgtttgc cgtcaatcaa tagtaaaaac
     3001 aagggagaac ttgcttggcc aaatatgatc ttgtgatcat cgggagcggt cccggcggat
     3061 atgtcgccgc cattcgcgcg agccagtggg ggctcaagac cgcggtagtg gaaaaggacc
     3121 cgtttctcgg tggtacctgc ctgcatgtag gctgtattcc caccaaagta tttttgcacc
     3181 acgccgatat ttacgacaac tttcagcgcg ccgaggaatt tggatttgaa gtaaaggacg
     3241 tcaagatcaa ttggccggcc atgctggccc gcaaggacaa aatcgtcaaa aaacatgcgg
     3301 gcggaatcgg catgctgttc aagaaaaata aagtcgattc gatcactggc tggggacgca
     3361 tcgccggtcc cggccgggtt tccgtcgaaa aagatggcca aaccaccgag ctcgaaacta
     3421 cctacacgat gatcgccacc ggctcggaag cccgctctct tcccggcgtg gaaattgacg
     3481 gtaagaccac cctcaccaac cgcgaaattc tgcaactgca agctattccc aagtcgctcg
     3541 tggtcgtcgg ttgcggcgcc gtgggcgtgg aatttgcttc catctaccgc acttttggcg
     3601 cggacgtaac gcttctcgaa gctctgcctc gcatcgtgcc gctcgaggac gaggaaattt
     3661 cgcccgaact tgaaaaggca tttaagaaaa agggcattca catcgaaact gaagccaaag
     3721 ttgaatccgt gaaaaaggac gccaagggtg ccaccgttac cttcaatgac aaaactggaa
     3781 agtcacagtc tgtctccgct gaaaaagttc ttatcgccgt gggacgccgc ccgctcaccg
     3841 aaaatatcgg cctcgaaaaa accaaggtca aactcgagcg cggattcatc catgccaacg
     3901 cctacctgga aaccgacgag cctcacctat atgccatcgg cgatatcgtt gccggacttc
     3961 cccagctcgc gcacgccgct tcaatggaag gaattatcgc cgtaggacgt atggcagggg
     4021 aagaggtgca gccttttgac cgcaagcgtt gcccgaacgc tacgtattgc gagccgcaga
     4081 ttggctcggt cggcctgacg gaaaagcagg ctcgcgatgc tggctacgaa gtcaaaaccg
     4141 gcaagttccc attccttggc aatagcaagg ccagtatcct cggccatcat gaaggcttca
     4201 tcaaagtagt tgcggaggcg aaatacggcg aaattctcgg tgtacacgcg atcggaccgc
     4261 tcgcgacgga aatcatcgct gagccggtta ccgcgcttgg cctcgaagcg acgctcgacg
     4321 atatggcggc ccagatccac gcccacccca cggtgtggga agccctcggc gacgccttca
     4381 actcggttcg cgggcttaca atcaactatt gagaagatct ctttaggggg tcggagcttc
     4441 agctctgaca aaaacagacg gcgcgatagc gccacccttt gcgccggttc tatcgcggca
     4501 tatggtcgaa gttcctgagg actccttgac gcaaggtgaa aaaatctgct ggctggtcga
     4561 tctgggcctg atcccctacg gcgctgcctg cgaattgcag cgccgactga tcgaggcccg
     4621 aaaagcccgc gcgattcccg acgtgctgct cctctgcgag catcctcacg tcgtcactct
     4681 tggccgcaac gccaagcgcg agcatttgct cgctagcaag cagttgctaa ccgaaatgaa
     4741 cgttgaactc cgctctaccg accgtggcgg cgacatcacc tatcacgggc caggccaaat
     4801 cgtcggctat ccaattctcg atctgacgaa gcatcgccgt gacgttcggt ggtacgtgga
     4861 gcaactcgag gaagcgatga ttcgcaccac cgccgatttc ggaatcatcg cgcgccgcat
     4921 cgagggccag cacggcgtat ggatcgatgg accttcgggc gaggaaaagt taggcgcact
     4981 cggcgtgcat ctaagccatt gggtcacttc gcacggcttc gcctacaacg tttcgactga
     5041 tctgcgctat tttgacttga tcgttccgtg cggcatcgcc gataagcgct ccacgtctct
     5101 tgaacatgca cttagccgcg acgtttccat cgaggaagcg cgcgcgcgtg tcgctctgca
     5161 tttctcgcag gtcttcgacc gcgaattagt cgccgtcgtt ccacgatcgt tggaggaaac
     5221 tctgagggct gcgctaaatt cgcagccaat cgaatccaca ttggtttaag gttagagcga
     5281 gctgcggatc gtcattctgc ttgccctgac ggaggaaggg agcggactgc accggtttct
     5341 gtgtttcgtt cgccgcgctt tatgtggcga acgccggtgc gcgcagtcga aggatctctc
     5401 ttctaggtca ccaagggaga aaacatgaca caatcaagcg ttgttgaatc cggccctcgg
     5461 ctgggcgatc cgacggccaa agaacacatg agctctaaac tcaccaacga ccaatacctc
     5521 gatctttact atttcatgct cctcaatcgc cggctggaag aacgcctcgt cagacttttc
     5581 cgccaaaata aaatcgtggg cggcgtctat tcgagcctcg gccaggaggc ggtttcgatt
     5641 ggcacggctt acgccctcga gccccgtgat tggctggcgc ccatgattcg caacatcggc
     5701 tcgcttctgg tgaagggatt caagccccgc gacattttca cccagcacat ggcaaaatat
     5761 acttcgccca cgcagggcaa ggatggcacc agccactttg gcgatctgaa gacgcgtcac
     5821 gtggtctcac cgatctccat gttaggcgat ctgattcccg tgatgaccgg cgtagctatg
     5881 gccggccgct acctcgggca gaatatcgtc gcgatgacct ggattggcga tggcggcagc
     5941 tccaccggcg catttcacga aggcctgaat ttggctgccc agcagcgcgc gcccttcgtc
     6001 ctgatcatcg aaaacaacca gtgggcctat tcgacgcctg ttgcgcggca ggtgccgctc
     6061 aaagaccttg ctgatcgcgc cgccgcctat ggcattgcag ccacaagcgt ggatggcaac
     6121 gacgtaatcg ccgtctatga cgccgcgaaa agtgcggtgg atcagtgccg tgccggccac
     6181 ggtccctgcg tgatcgaagt gaaaaccatg cgcatgaaag gccacgcgca gcatgacgca
     6241 gccgactacg ttcccaaagc catgatggat tactggaaag cgcaagatcc gatcgcccgc
     6301 tatgagaatt atcttacgca gcaccatctg ttggattcag cgaagaaatc ggcgatcgat
     6361 gcccgtattg agcgcgaatt ggatgaggat caaaagttcg ctgaagaatc gcccatgccg
     6421 ccgcccgaac tcgcagaaca gggagcctac tgcgaaggct gccatacgat ccaggcaaac
     6481 tggcagcgtc ccaaaaagga agtgacgccg cctgcatcga gcgtcgaagc gatctggaaa
     6541 gtggcggatt tcggcgcatt caagaggccc gccgtgcctg aggccgccac cgagcatgaa
     6601 aaccgcgatg gtaacggcgc taattccttc gacgaactac catccgctga aacccaaatc
     6661 gaaaagcaag ctcggctacg ggttccgttc ggccgtggcc ccaaagaccg ctcagtcgaa
     6721 ctcgaacgcc gcgataaagt cgccgaacgc cccccgcgcc atcacaagcc ccacaaacgg
     6781 cggaggcgct gatgcctgaa atcaccatgc tcgaagccat ccgtgcggcg ctctttgaag
     6841 aaatggagcg tgatccagcg gttgtgaccc tgggcgagga tattggtgtc tatggcggcg
     6901 catttaaggc caccgagggt ctgctcgcaa aatttggccc tgagcgcgta atggataccc
     6961 caattagtga gactgcgatt gttggggcgg cctgcggcat gagctatctg gggttgcgcc
     7021 ccgtggttga aatgcagttc atcgacttca tcgcttgctg tttcaatcag gtcactaatt
     7081 ttgttgccaa atctcactac cgctggggcg cgcccgttcc catcgtggtc cgcgggccct
     7141 ccggaggcgg cgttcacggc ggcccatttc attccgcgaa cccggaaatg tatttcgtgc
     7201 acacgccggg gttgaaggtc gtgtatcctt ccacggctta cgatgccaag ggtctgctca
     7261 aatccgcgat tcgggacaac aatcctgtct tgtttttcga gcacaaattt ctttatcgaa
     7321 ggctcaagga agaattgccg tccgaggact acaccgttcc tcttggtaaa gctgtggtcc
     7381 gccgcgaggg aaaggatctt actatcctca gttatgcggc catgatgctt cccactctcg
     7441 atgcggccca gcagctctca cgcgagggaa tcgaagcgga agtgattgat ttacgcacgc
     7501 ttttgcctct ggaccgggag gctattctgg cttccgtaaa gaagacaaat aaactactga
     7561 ttgttcacga agatacgcgc accggtggca tcgccggaga aatcgccgcc atcgtgtgtg
     7621 agagtgcttt tggggatttg gacgggccca tcacgcgcgt cacggcgctc gataccccgg
     7681 tgccctacgc gcccagtctc gaggagcggt ttctgccgaa cgccgaaaaa gttgccgtcg
     7741 cagcccggga attggcaaaa tactaatggt gggacgagct ccggacatgg gggtagaagc
     7801 gtgggctttc gtactgtggg gatatctcgt ccccgtcaac aacgcaagga accttaacac
     7861 agccgcggga atctaaccgg gaagaaagca ggtattgcgc cttgggaaaa acgaaggggt
     7921 tgaacaaagc cgagttcgaa gtcgggtccg aggttcgcat cgcggaccgc gcattcctgg
     7981 aggaatttct tgaggctggc cagtatcaca acgagctgga gcccgagcag gtcgaatacg
     8041 ccgggcgggt tgccaaggta aagaatatcg agttcttcca cggcggcgac gagatttata
     8101 ccctcgaagg aattccgggc gtctggcacg aggaatgttt gagtcccgcc tgactccaaa
     8161 agtccacaca ccttcctact tgcacttctg ccttcgtgca ttaagatgga actgagagaa
     8221 tcggttcaca tttagcgtta gctgggtcgt tcagccagcg ttatcagaac ccgagacgtc
     8281 ccgaaacatg tagggcaacg atgagttctt ctcgacacaa gcgccgagta gaaatcatcg
     8341 caagctattg aaaacaaaag acgcgggtgc ccgctacccg tcacactttc gaggcccgaa
     8401 gcttcaagac tttcggtcga ttctttgacc gaaaggtgga aggtttatcg gaggattagc
     8461 catggctgtt gacgtcatta tgccccagat gggcgaaagc atcttcgaag gtaccatcac
     8521 caagtggctg aaaaagcccg gtgacaaaat agagcgagac gaaccgctct ttgagatctc
     8581 aaccgacaag gtggatgccg aaattccgtc cccttccgcc ggcgtgctga aggaaatcaa
     8641 agtcaacgaa gggcaaaccg tcccgattca gacggttgtg gccctaattg aggcagcggg
     8701 cgcaaccagc caaccggcag cgacgtctcc tccgactgct aagcccgacg cgaaatcaag
     8761 ttccccggcc agcagttctg cgagcactcc gccggcgccg gctgcgaagc ccgcagcggt
     8821 gaaacccggg ccagctcccg ccgccacgcc tcccgttggc tcgaaagttc ccgctcccgt
     8881 tgccgctcca agcttctcgc aaccttcgag gcataatggc ggcgacaaaa tccgtagctc
     8941 accgctggtt cggcgcatcg cccgtgaaaa tcaaattgac cttactcaag tatcgggaac
     9001 tggcgcggga ggccgcgtca gcaagcgcga catcctggcg gccgctgagg gtggtgcgac
     9061 gacatctgcc gcaccagctc ccgcgccttc aggcgtgcca tcgcatccag cgatcgaagt
     9121 cggcgtcccg cgcgagcgca tttactttgg ccactacgaa gtgcagccca tgtccaacat
     9181 gcggcagaaa atcgccgaac acatggtgct ttcgaagcgc gtttcgccac atgtgtactc
     9241 ggtggaggaa gtggacgcca cggggatcgc caagctgcgc gcaaatgcga aggagaaatt
     9301 tgaaaaggaa tcgggcacca agctgacttt catgccgttt ttcatccgcg ctgcggtgga
     9361 agcgctgcgt gcttttccca ccgtcaactc atctgtggat ggcacgaata ttgtcctgca
     9421 caaggagtgc aatatcggca ttgcagtggc gctggattgg ggactgatcg ttcccgtgat
     9481 caagaatgcc gaggagaaaa actttttcgg tctcgcgcgc gctgtcaccg atctagcgga
     9541 gcgcgcccgc acaaaaaaat tgaagcccga ggaagtaacc gagagcactt tttcaatcac
     9601 gaatccgggc gtcttcggcg gattgttcgg gcttcccgta atcaatcagc cgaacgttgc
     9661 aattctcgga ctgggcacga ttgaaaagcg cccggtggtg atcgacgatg ccatcgccat
     9721 ccgctcgatg gtttatctga cgctctctta cgatcatcgc gcggttgacg gcgccatcgc
     9781 gcaccaattc atgggccacg tgaagcacac gctgcaggat tggacagaaa gcatttttta
     9841 ggcaacttga gtgcgaagca ggcgctcctt gctgcatcca acctctgcta tgtcgcgctc
     9901 gcacgcgctt gaactttggg gtgttccgcc ctggaaaatc gagttcactc caccgaagcg
     9961 ttccctcccg tccacggtgg atttcgcagt ggtgggcgca ggttttacgg gcctcgctgc
    10021 ggcagcctgg ttgcgcattc tggctccggg gaaatcggta gcggttctcg aagcgggacg
    10081 catcggaaac ggcgccagca ggcgcacagg aggattggtc ctcagcgaat ccgcggcagg
    10141 tgatttgcct ggacttggcg acgttctctc tggactaaaa aagattctcc gcaaacttcg
    10201 cgttgagtcc gatcttctgc ttcccggcgc gtgggaaatt gcacacagcg atcacgctga
    10261 accctcggac aacgcgcggc ccctggaggg gcgttctcca attcagtggc gcgacttcga
    10321 ttctcgggag attcgggtgg caggcaccgt tccaggtgga acgctcgatc cgggaaagca
    10381 ggtcagcgga ctcgcacgcg ccgcggtgag tttaggcgcg gtcatcgcag aaaactgcgg
    10441 tattcgcaaa atcgaatgga ccactcctcc gacactgcac cacaccggcg ggttcttgcg
    10501 cgcgaagaaa attcttctgg cgacgaatgc cctttcgctc gagatttccg ggctacgcaa
    10561 atccgttatc caaccgaaac tgacgctcgc tgcgcggact gccccgatga gtgagaaaga
    10621 gttgggggaa atggggctag cggggcgcaa gccgttttac acgatagact ttccatattt
    10681 gtggggtcga gtatgcgccg atcggtcgat cgtgtggggg gcgggactgg tggacgcgcc
    10741 gagtttgggc gaactcgaag atgttgatgt ttcgaagggc cgcgccgcca aaatgtttgc
    10801 ttcatttgaa aaacgggttc acggactgca tcccgcgctt gccaaagcgc gttttacaca
    10861 caaatgggga ggccccatag ccttccgaga aaattttaac ccggtgtttg ctcatcatcc
    10921 agaaagcgcg aatggtatcg ttctcgggat ttttgcgggc cacggtgtgg cgctgtcggt
    10981 ctatctgggg acctgggccg cggaggcatt gttggggaat agggcgttgc cagtctgggg
    11041 ccagcttaag tcgaaataga gtcgcagaca aaggcaaagt tagtgtgagc ctgttcgtac
    11101 tgccttcgtg gtcgctaaca atttaatata atggcaaaaa agcgaaacaa ccgagctcag
    11161 ccatcgctct ttcctccgaa gaaaaccgaa gtgccgtcaa ttcccgcgga ggaggcgctg
    11221 agcttcttgc gcgaaacgcg cggggtagtc acgtggaccg cccgcgacat ggccgattcg
    11281 ctgaaaatca gcgtggcgga cgcgaaacgc gtgcttccga ttctcgagct gcaaggctac
    11341 gtgaagccgg ccggggcgaa cgaatggatg accacgttgg ccggcgaaga ggtttctgga
    11401 tcgaaggctc cgcggtttac tcgtgaacgc gttgctcaag ccttgaacga tcttcacgat
    11461 cggattgcgg aaatcaaccg caattccaaa acgccttaca aaattaccga ggctgtcgca
    11521 ttcggggatt tcttaagcga taggccgcgc gttcaggccg ctgaagcggg aattgggctg
    11581 gaacggcgag aaattggcgc agatgacgca ggggacggag tgcagcagcg agatttcctt
    11641 cgccagctac atgccaaggg cggcgtgatt caggtgcgcc catacgaacc ctggatgagc
    11701 gagcgaagac accgaagtct gttgcgttga agatcgaagg cctgcaggaa cgcaagagcg
    11761 ccaatcggga gattggcgtc cccaggaagt cctagacgtt gaaaatgtgc accaccgcta
    11821 gcgccaattg ttgcgttcgc gaagggcggt gatgtgggcg atgtggtggc ggccgtgcca
    11881 ggcataaagc tgtaaattat tttcgagggt cacggtgccg cgttcgggat ggtgaaactt
    11941 gcgctgccag tcggtgaccg tcagtgagtt taagagcgat atccagcgct tgtgcaaatt
    12001 ttccagcaac agaagcgatg gctcgaccgg cgcttttgcg tcggccaact cggcccagcg
    12061 agcttcgtcg tacactttga tcgtggggct gtcttcggtg agggcgagtt tgaaacgcac
    12121 gaaagcattc atatggctgt cggggaggtg atggacgact tggcgaaccg cccagccgcc
    12181 aggacggtag ggagtatcga gctgcgcttc atttagtcgg gcgatggcgg ccttcaatct
    12241 ttccggtgtt tcggcgattg cagcgatgag cgcaccacgc tgaatgtccg tgacattttc
    12301 agcgggctga aacttgccga tcgggtatcg caggtctgtt tccacggcgg ttcctccgct
    12361 ttgcctggtg tgaaaggcgc agcgcctccg caacttcaaa cattattaat gcgcgaggct
    12421 gcttcggcaa gatggcagac ggcgagcaaa gaatgctcgt ggggcgcagc atgctgcgcc
    12481 cgttgcaagc agtgggctgg ccctcagaaa atcagcttca gggcgaattg tatctgacgc
    12541 gaggtggtgc tcgtagacgt aatcaagcct gagttccccg cgatgaagga accggcagca
    12601 gcccccgctc cgcccgctcc tgaaaaaacg gcaccgttcg gctgtccgag attcgtatgg
    12661 ttgatgatgt taaaaaattc cgcccggaac tctgtgttta acttttccgt aatcctcgtc
    12721 tgcttgatta ttgagaagtc gaggtccaag aggcccggac cgattaagga atcccttcga
    12781 acgttgccga gcgtggcgaa cggttgaacc gcgtagcacg ccgggttgaa ccactgcacc
    12841 gtcctctgct gagcattgac ggtatcggtt atctgagacg gatggcaccc cggcgctccc
    12901 gaataattcg ggcgatcggc tgttagcccg cccgtgttcg caacctgtgg aagcagtcca
    12961 ttcgtcacat tgactggata acccgtcgtc gcgctgagga cttcgcctag ctgccagcct
    13021 gaaaccaatc ggttgcccgt gaaaggcagg gtgtaaatgc tgttaacccg caaggcgtgt
    13081 gtgacattga aggtgcacgg accgcgatca aaaccctggt tgcgggcgtc cagtatttcg
    13141 gcagacgcct gttcgagacc ggaactggcg gaaccatcgt caatgcatct tgaaaacgtg
    13201 tatgacactt gaccttgcag attccggctg acctggcgcg taagactcgc cactaaacca
    13261 ttgtaagtag aatggctcgt aggagcagtg ttgtccaagc cgttgaaagc aggatttggc
    13321 acagggccct taaagaagca cgaggggctc gtgcaggtag gtgagacagg attcccggct
    13381 gcgtcgacga gcgacgggct attctgattc ctttggttat acagatggac gccttgtgcg
    13441 ccgacatagc ctagcgatgc aaccatgcca tgtccaacat cacgctgcac ggtcaggttg
    13501 tactgcattt cgtacggtcc actatccgac gtgtagtcga ggccatagac tgtgccgaat
    13561 ggaggattca agcatgccga acccaccggt ccgcacgccg ctggatgcgg gaagagcgaa
    13621 accgcgggag gcccggcaag gaccacggtt tgggacgggg gattctgaga atacgcgttg
    13681 aggtacgttc gcgcggcaat tggctcgtgg aagattccga aaccagcgcg gatcgacgtc
    13741 ttatgatccg agaaggggtc ccaagccagg ccgacgcgag gatcaaaatt caatgcgtcc
    13801 gggttcgact taagaacgtg atgtacctgt gtgaagccgg gcgaggacaa tggattcagg
    13861 atcgcattgc acggttcagt cacgcagacc gggttggtct catagtcata tcgcaggccg
    13921 atattgattg tcaggttcga ctttatcttc caatcgtcct ggatgtaggg gaagaattga
    13981 atctcgcggg agtaacgtgt cctagtgaag cctggatgcg gattggtctc gccctggaat
    14041 tgcacaggcg tgcttgccag aaaacccgca atgctgccga agacgaatag cccgccctcg
    14101 cgacccaccg cgatgttgtt ttgaactcta gatacggacg cgccgacctt gagactgtga
    14161 gcaccgtggc tccaaaagaa atcatcgccg ccggtgaact tattctgaat tagatgccat
    14221 cgtgccgttg ggcccgggcc cacgttcgag gaaccgccgg caacgactat attgccatct
    14281 tcgcggtcgc cggggctgta ggccggagaa ccattgaaaa gcgctcccag gtagaactgc
    14341 agcggatccg tggctggatc cgacaagaat ttctggtccg agcgttcatt ggttcgcgag
    14401 aagctgaagc gaacctcgtt gattgacgtc gctgaaatga tgtgtttttc ctcgagagtg
    14461 aaaaactgat caccgccgtg aaacgccgcc ggccagttgg acaagttcga gaagggagca
    14521 ggaagaagca gataggcggt gtcggaaatg tatctgccaa acaaggagtc cttcgagccg
    14581 agggtgtagt ccacacgccc aagcaggtag ttctcgctgg taggctcatt cgaaacctca
    14641 gtgatgagtt ggttgccgct gggcaaaccg cgaaacggtc cagcaccgac gatttgttcg
    14701 ggtgaggtta gggaaggaac ttggggatag agctgtcccg gtagatacgg gacaacgcca
    14761 ggattgatcg ccacacgagt gcgtgctgcc ccgacctgta cacagccaaa ctggtagggc
    14821 acctccgcgc cgcccccgcc tgcgccggga cagctgttag ctgtattcag gccgagcgag
    14881 tcgcggtctg gaacaaattg atttcccgta atgcccaagg ccgatcgaag accctcgtaa
    14941 ttcacgaaaa agaaggcctt gtccttcttg acggggccgc ccagactgcc accaaactgg
    15001 ttcctgcgga agggtggtat tttcgatcca tcaaagaaat tgcgcgcgtc gagcgcgctg
    15061 ttgcgcaaat attcgtaagc ggaaccgtga aaatcgttgg tgccagactt gctgacggca
    15121 ttcatggccg cgccggtccc gccgaattgc gcgctgtacg tgttggttag cagctgaaac
    15181 tcctgaatgg cgtcgacgcc cagggaattg cctgtgattg acgcacccgc tcccttttcc
    15241 caatagccct gcagatcttg attgtcgagc aggaagactt gcccaacggg cctggatccg
    15301 gcaaccgagt aggtgtccga gggtccgtaa aatcccgaag atatcccgcc agcgccgccg
    15361 gttacgaacg cctgtttcac cgtgagaaca ccgggcgcga gcgagagaag ttgctcgaaa
    15421 tttcggccgt tcagaggcaa ctggctcatc tgttgcgacg tcaccagcga tgagacggaa
    15481 gcagtttgcg tttcaaccct ggaaacttcg ccttccacgt tgacggtctg cgaaacttgc
    15541 ccgacgggca gcgagaaatc gacgacgagg ttggcgccaa ccgtgagtgt gatccccttg
    15601 tggacgacgg tttggaagcc tgtttgagag gtctgcagct cgtaggttcc aatgggcagg
    15661 tcgggaattc tgtagcggcc ggacgcatcg gtgacggtgg actgggtgat attcgttccc
    15721 acgtttctcg cctggacggt tgcggacacc agtgctgcgc ctgaactgtc ggt
//
LOCUS       DATNDT010000453         7596 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1259614, whole genome shotgun sequence.
ACCESSION   DATNDT010000453 DATNDT010000000
VERSION     DATNDT010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1259614"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VN788_14795"
     rRNA            complement(<1..125)
                     /locus_tag="VN788_14795"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(790..1275)
                     /locus_tag="VN788_14800"
     CDS             complement(790..1275)
                     /locus_tag="VN788_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21848.1"
                     /translation="MATPLVDDYTRWTESNAPGGAAHDFCVRFDIVRVHRALAKIACS
                     LALLCYGELCRKITGFADVCEYVLRGKGERVERATMDLNEFSGLDASADYHYAHLWMK
                     QSRLVCILSIYGARISFDLGRVPERSSWRSITAACKTDGKKCAILNGAVADQVAATVM
                     G"
     gene            2044..2529
                     /locus_tag="VN788_14805"
     CDS             2044..2529
                     /locus_tag="VN788_14805"
                     /inference="COORDINATES: protein motif:HMM:NF025056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003953 - NAD+ nucleosidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HXU21849.1"
                     /translation="MGLNVFLSFSVDPGDQVLIWRLQTLATAQGIALYVPLWQVVENR
                     RSGPLPQVVRAAIDRADCVLAIITTRTGPKVRDEINYALQKNKLIVPLIEKSVPFPPI
                     FKKLPQVFSFTQGEPPGRLESEVVQFLKEQQFKKEAGQQLGAVIAIGLGLLALAALAQ
                     D"
     gene            2536..3030
                     /locus_tag="VN788_14810"
     CDS             2536..3030
                     /locus_tag="VN788_14810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21850.1"
                     /translation="MRPTAVVDSSALINLVHLGLIQNLRLYFDEIYVPKMVQTEVNRK
                     GRFRYRLNKLYKQRVLTRCLAGNRVNVKLLAPLDEGEAEALVQASEIGAHVFIGDEPP
                     AREMSERRGLRPIGTARLLARFHLEGYSDEPRLLVQRLRNEVAFRISDEIVDAAIQTA
                     HEPI"
     gene            3756..4877
                     /locus_tag="VN788_14815"
     CDS             3756..4877
                     /locus_tag="VN788_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HXU21851.1"
                     /translation="MNTQELFTPFTLNSVTLRNRTVMAPMTRNHSPGGVPGPDVAAYY
                     RRRAEGGVGLIITEGTAPNHPEAKNMPNVPHMYGEQALAGWTRVVEGVHAAGGRIFSQ
                     LWHVGAVQGHTEPKLPVAPISPSGFLKPDTKIGEPMTLAEIEGMIAAYAQAAEDAQRV
                     GFDGIELHGAHGYLIDQFFWDGTNQRTDKYGGDLVERTRFAVEIIQECRRRVGPKFPI
                     QLRFSQWKLQDYGARLATTRQDLERFLEPLAASGLDAFHCSTRRFWEPEFEGSDLNLA
                     GWTKKLTGKPTITVGSVSLDVDFTVSLGRASAPKADGNGAGRTAHMEVLGEMLARGDF
                     DFVAVGRAILADPAWAAKVRDSDFSKLSPFTPDVLKTLV"
     gene            4894..5190
                     /locus_tag="VN788_14820"
     CDS             4894..5190
                     /locus_tag="VN788_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014917701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI-like protein"
                     /protein_id="HXU21852.1"
                     /translation="MSMAYFAMTYNLVDDYMTRRGAFREEHLKLAADAHARGELILGG
                     AFADPPDKALIIFRAKDSKVAESFARNDPYVLNGLVKDWEIRPWNVVIGNTEVG"
     gene            5249..5602
                     /locus_tag="VN788_14825"
     CDS             5249..5602
                     /locus_tag="VN788_14825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU21853.1"
                     /translation="MSLQGTFREVGEIAVIDFSGTITLGPSTATLRKMVRELVAKGYR
                     KILLNLGEVDYIDSAGLGELVSAHATVQNARGELKLVELNKHVRDLIQITRLYTVFDV
                     QPTEAAAIRSFNTTV"
     gene            5786..6028
                     /locus_tag="VN788_14830"
     CDS             5786..6028
                     /locus_tag="VN788_14830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21854.1"
                     /translation="MHCPQCGWPDIGKMPAGVRLIKHNDKTVLLGHLIYVPIDAVLCS
                     VCEEMKSAVESNPWFREAHREYLEQVKVKKRILRGT"
     gene            6523..>7596
                     /locus_tag="VN788_14835"
     CDS             6523..>7596
                     /locus_tag="VN788_14835"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HXU21855.1"
                     /translation="MARLTFPFSRSVCRWLLQKIYQSIGHPRVRLSLARGEAFPPGGT
                     EILTTVRIADLRTLATLVVTPEIGFGEGYSKGRIEVQGDLVRFLDAVLASIRDANAIG
                     WYSKLVSRWFDLAQRNSVRGSLRNIHHHYDVTADFYKLWLDPQLVYTCAYFPEPFSSL
                     EDAQIAKMDHVCRKIWLQPGERVVEAGCGWGALALHMAGKYGAKVRAFNISKEQILVA
                     RERAKQAGLERQVEFIEDDYRNITGHYDAFVSVGMLEHIGTQNYETLGEVVHRTIGDA
                     GRGILHFIGRNFPYSFSPWIRKRIFPGACAPSLAQAMAVLQPWNFSVLDVENLRNHYV
                     RTLELWLDRFESAWDSVVELLGTE"
BASE COUNT         1706 a         2136 c         2084 g         1670 t
ORIGIN      
        1 ttccccattc ggacatctcc ggatcaaggc ctgtttgcgg ctccccgaag cttttcgcag
       61 cttaccgcgt ccttcatcgc ctgttggcgc caaggcatcc accgtacgcc cttagtagct
      121 tgaccataaa acttacctaa cgttactatc cgatgaggat agtcatcccg aggagcatcg
      181 cgacgaggga cctttcggct ctcctcgcac tgcttctcag tcgtgcgttt cctcaagccc
      241 ttgctatgac ttgaagacca cacgagcgaa ggcaagttcg cctttccaca taacgctact
      301 ctcgagcaaa aatttgcggt ttaccgcgcc atgcgaacct tgaaattcgc ataacgcgat
      361 gcctacttcc ttcggttgtc aaagaacatc cgtttcgatc aactgtttcc gatcggaacg
      421 tctccgctcc cggcggagcg cggtaccggt ttggagccga tcccgcgaaa aaaacctgca
      481 aacaataaaa aacccggcga cgagcgccgg gcgaatccgt ccttcttctg agtcgctttt
      541 tccgcgcgac tccaagacgg atgtgctcga agctcgattt ctttctatcc ggtatttgta
      601 accaatctca cctctggatg agtcggtaag ccccggccgt cagcagacta cgcccctaat
      661 cagggctcta ccaacttcga agcagtatat caaaatccaa aattccgtca agttgcattc
      721 acgatttctt cacatccccg tcgcacttcc cctgtgaatt tcaccgcctc ctgtggaaat
      781 tgacgtgggc tatcccatta ctgttgctgc aacttggtcg gcaacggcgc cattcagaat
      841 cgcacatttc ttcccgtcgg tcttgcaggc cgccgtaata cttcgccagg acgacctctc
      901 gggcacccgg ccaaggtcaa acgagatccg agcgccatag atgctgagaa tgcataccaa
      961 tctactctgc ttcatccaca aatgcgcata gtggtaatcc gccgaggcat ccagtccgct
     1021 gaactcattt aggtccatag tcgcgcgctc gactcgttcg cccttgcctc gcaaaacgta
     1081 ttcgcacacg tccgcgaatc cagtaatctt gcggcataac tcgccataac acaaaagcgc
     1141 cagggagcac gcaatctttg cgagtgcgcg atgtactcta acgatgtcga agcgtacaca
     1201 aaaatcgtgg gccgcacctc ctggagcgtt ggattcggtc catcgcgtat agtcgtcaac
     1261 caaaggggtc gccaagagaa ttcgcagatt agttaggaag aacttcgctt caccagggtc
     1321 tcgagctcgt aggaagagct taccatcggg ctgtcgggaa attctcggcc agaaatcagg
     1381 gtcctcgccg atttcagaga gaggagcggt cctcaactcg atcttgtcta tcgagccatc
     1441 ggccagcgca gcgcgtaggc aatccagaaa gcggcgatgc tccttcgggc ttgcgcctcg
     1501 ccgacgagca aggcgccgac cgccaacgac tgcaatttca attgacggga gttgctcggc
     1561 gggcatgcct gcgcgaacac aaacttctga gtatcctccc tgcggattgc gaagaaatac
     1621 atactcggtc ttgatcattc cgctggacgg tcggcgcttg tgtcggcctc ggggtccgtg
     1681 ctctacagtg cataaaccaa aaggggaacg ccgcgaaagt tcggtctcca aacgagacag
     1741 tgcttgctgg caggggacac aagcgggcac ggccagatct ctcgtccctc ctagcgcgcg
     1801 cggaaaaacg tggtcactgg tcagatcgcc ggatttagac aagcaccaag aacaaagcaa
     1861 ttgggacatc gtcggcaaat gcaaaaccat ctgcatcaag tatagctgta ccgtgaacta
     1921 attcgcacaa ccttctcggt ggatttggtg cgtttcagac ttctcttcag gccacatgct
     1981 ctggagtgct tcacgacttg cccacgattc ctggaatggc aaaactcggt taaactctct
     2041 aacatgggct taaacgtttt ccttagtttc agcgtcgatc cgggggatca ggtcctgatc
     2101 tggcgacttc agacattggc aactgcgcaa gggatagctc tttacgttcc cctgtggcag
     2161 gtggtcgaga atcgccgctc tgggcccttg ccgcaggtcg tgcgtgcagc aattgaccgt
     2221 gcagattgcg ttctggcgat tattacgaca cgcactgggc cgaaggtaag ggatgagatc
     2281 aattacgctc tgcagaaaaa caaactgatc gtgccgctta ttgaaaaatc ggttccgttc
     2341 ccacccattt tcaagaagct tcctcaggtc ttctccttca ctcaaggcga gccgcccggc
     2401 cgactggaat cggaggttgt ccagttcttg aaggaacagc aattcaagaa ggaagccggg
     2461 cagcagcttg gggcagtaat agctatcggg ctaggcctgt tggctttggc cgcactagca
     2521 caagactagc gggtgatgag gcccacggcc gtagttgaca gctccgcgct catcaacctg
     2581 gttcacctag ggctaatcca aaaccttcga ctttacttcg acgaaatata tgttccgaag
     2641 atggtccaaa ctgaagtaaa tcgcaagggc agattccgct atcgcctcaa caagctttat
     2701 aagcaacgcg tactcaccag atgtctggcg gggaacaggg tcaatgtgaa gctgctggca
     2761 ccgctagatg agggtgaggc tgaagccctc gtgcaagcat cggagattgg ggcacacgtc
     2821 ttcatcggcg atgaacctcc tgcgagagaa atgagcgaac gcagaggctt gcgcccgatc
     2881 gggaccgctc ggcttcttgc gcggtttcac ctcgaaggct actctgacga gccacgcctc
     2941 ctggtacaac gcctccggaa cgaggtcgcc tttcggatat cggacgagat agttgacgct
     3001 gcgatccaaa cagcgcacga accgatctga ctttagtaat tcgtcgtcaa cacactccgg
     3061 acatcggtgt cgttcccaag cccagactcc agtccacagg tttcttctgc cttgcattcc
     3121 taattcggca atccgataaa gattgccgtc ccgagcgcat tgcgtgagcc gagggatcta
     3181 tctaaatcgc gatagtccac aggaaatttt tcccttgcat ttcggaaact tagttaggct
     3241 gaagttgcga tgtattcgaa cgcaacattt cggccgcaac cgagcgcctc agcgtccatc
     3301 gccgagccac gcctcgacca gtccgacggc cagggtagga gggcggggct taagccccgc
     3361 cgcagaaacg tcgcccgaaa ggccttccat tgcgccgttt ttctcgcggc aagtatccga
     3421 ggctcgtctg ctcgggagcc ccgagataag aatggagcgc atcgcgcttc ccaatccgct
     3481 ggctctgtcg aggcaagtac tcgcctctcg aagacccaaa ccacccaacc cacactgtcc
     3541 cgaacctccg atctccggcc tgcaacatcg agtttctact cggcacagtg gacgagtaga
     3601 acccacatta aatgccgtgc aatcagcgac ttagtttttt ctactcgaca cttttctcga
     3661 agccttcatc tgccggtttc agcctccgaa tccacctgcg gcgcggacgc atcgcataca
     3721 cttcgatctc aaatccgtgt ctcgaaagga ttgcggtgaa cactcaggag cttttcacac
     3781 cctttacgct aaacagcgtc acgctgcgga atcgcacagt gatggcgccg atgacgcgca
     3841 atcactcacc gggcggcgtg ccggggccgg acgtggctgc gtactatcgc cggcgcgccg
     3901 agggcggcgt tggattgatc atcaccgagg gcacggcgcc gaatcacccg gaagcgaaaa
     3961 acatgcccaa cgttccccac atgtatggtg agcaagcgct tgccgggtgg acgcgagttg
     4021 tggaaggcgt ccacgcagcg ggcggacgaa tcttttcgca gctctggcat gttggagcgg
     4081 tgcaaggaca caccgagccg aagcttccag tagcgccaat cagtccatcg ggattcttga
     4141 agccggacac aaaaatcggc gagccgatga cgctggccga gatcgaaggc atgatcgcag
     4201 cctacgcgca agcggccgag gatgctcaac gcgtgggctt cgatggcatt gaactccacg
     4261 gcgctcacgg ctacttgatc gaccaattct tttgggatgg caccaaccag cgcaccgata
     4321 aatatggcgg cgatctggtg gaacgcacac gattcgcggt ggaaataatt caggagtgtc
     4381 gtcggcgcgt ggggccgaaa tttccgattc aattgcggtt ttcgcagtgg aaattgcaag
     4441 actatggggc gcgactggcc accacgcggc aggatttgga gcgatttctc gagccgctgg
     4501 ccgcatccgg actcgacgcg tttcattgct cgacgcgccg attttgggaa cctgaatttg
     4561 agggcagcga tctgaatctt gcgggctgga caaaaaagct aacgggcaaa cccacgatta
     4621 ctgtcgggtc ggtctcgctg gatgttgatt tcacggtctc actgggaaga gcgtcagctc
     4681 ccaaagctga tggcaacggc gccgggcgga ccgcgcacat ggaagttcta ggcgaaatgc
     4741 tcgctcgcgg agatttcgat ttcgtggccg tgggtcgggc gatactcgct gacccggcgt
     4801 gggccgcgaa agtgcgcgac agcgattttt ccaagcttag ccccttcacc ccagatgtgc
     4861 taaaaacgct ggtctagact gagtgcgagg acaatgagca tggcctactt cgcgatgacc
     4921 tacaatttgg ttgacgatta catgacgcgc cgcggcgcct tccgcgaaga acatttgaag
     4981 ctagccgcgg acgcgcatgc ccggggtgaa ctcatattgg gaggcgcgtt cgccgatccg
     5041 ccggacaagg cgctcataat ttttcgcgcc aaggattcaa aggtagccga atcattcgcg
     5101 cgcaacgatc catatgtcct caatggtttg gttaaagact gggaaatacg cccctggaac
     5161 gtcgtaatcg ggaacacgga agttggctga cagttggttg gctcacaggc gtggcattga
     5221 tcagacggct atcgatctct aagtttccat gtctttgcaa ggaacattcc gcgaggtggg
     5281 tgaaatcgct gtaatcgatt tcagcggcac gattaccctt ggcccaagta cggcaaccct
     5341 gcgaaagatg gtgcgcgaat tggtcgcaaa gggatatcgc aagatccttt tgaatttggg
     5401 tgaggtggat tatatcgata gcgcagggct cggtgaactt gtgagcgccc atgcgacagt
     5461 tcaaaacgcg cgcggcgagt tgaagctcgt tgaactgaat aagcacgtgc gggatctgat
     5521 tcagattacg cggctttaca ccgtcttcga cgtgcaaccc accgaagccg cagccatccg
     5581 aagtttcaac acgaccgtct agccacgact tcctcagtgc ctctgaatcg ttccaagtac
     5641 taaagtccca gaaacccagg agtaccaacg ggaacagaat cccaactgtt gttccattcg
     5701 ccgatccgct cagcgctggc ataaagaaga ccgcgataag cggcgtctta gccgcttcgg
     5761 aatgaggacc gtcacggagg atggcatgca ctgcccgcaa tgcggatggc cggatatcgg
     5821 caaaatgcca gcaggggttc ggttgataaa acacaatgac aaaactgtcc tgctggggca
     5881 cctaatttac gtgcccatag atgccgtgtt gtgttcggta tgcgaagaga tgaaatctgc
     5941 tgtggagtcc aatccgtggt ttcgcgaggc acatcgcgaa tacctggagc aggtcaaggt
     6001 caagaagcgc attctgcgcg ggacttaagt aagaacgtta cgggtcagtc gtgcgaatcc
     6061 cctagtttcg ctcgattcgc tagccggcgt gagcagcttt ttgcccttcg aatgctttgc
     6121 acgggcacga aacgcgttcc gtttttttgg ggtctggatt gggtcgcaag gccgtgcagc
     6181 cgtaggttct atgtaaggac tgcggatgtc cgcaaacaca gatgtcattc cgaaccataa
     6241 ttccttcctc ctaacgcagt ctattagtaa tgacgcggag cgcaatcagg tattccccct
     6301 acgtaccgga tacgccgaga agttggacac gcgagcttga agtaggctct ttaggtcggc
     6361 actccccacg catgtgcacg gtaagcctct gcgtcttttc cgtttacaat cgcgttgtcg
     6421 agcggggttc cggcttggtg ggcgcgaaag agcggtgttg ctggaagccg aagtagcatg
     6481 cgtggtaagt ttattactag ccacaatcag gagcgttcaa ccatggctcg gctaacgttc
     6541 cctttctctc gttcagtttg ccgttggttg ctgcagaaaa tttaccagtc gattggccat
     6601 ccccgcgtgc gactctcatt agcgcgaggc gaagcattcc cgcccggcgg gacggaaata
     6661 ctgacaactg taagaattgc cgacttgcgg accctcgcaa ccttggtcgt cactcccgag
     6721 attgggtttg gcgaaggcta tagcaaaggg cgaatcgagg tgcagggcga tctcgtccgg
     6781 tttcttgacg ctgtcctcgc gtcgatccga gacgcgaacg cgattggctg gtactccaag
     6841 ctggtctcgc gatggttcga tctagcgcag cgcaactcgg ttcgcgggtc gttaagaaat
     6901 attcatcacc attacgacgt gaccgccgac ttttacaagt tgtggctgga tcctcaactg
     6961 gtttacacat gcgcctattt ccccgagccg ttctccagtc tcgaagacgc gcagattgcg
     7021 aagatggacc acgtctgccg caagatctgg ctccagccgg gtgagcgagt ggtggaagcg
     7081 ggctgcggtt ggggggcact cgcgcttcac atggccggga agtacggggc caaggtgcgg
     7141 gcattcaaca tctcgaagga gcaaatcctc gttgctcgcg agcgggcaaa gcaagcgggg
     7201 ctggagcgcc aggtggaatt catcgaggat gactaccgga atatcactgg acactacgat
     7261 gcttttgttt cggtaggaat gctggagcac attgggactc aaaactacga aactctcggg
     7321 gaagtcgtcc atcggaccat tggcgatgcc ggacgcggta ttctccattt tatcggacga
     7381 aatttcccgt actcgttcag tccttggatt cgaaaacgca ttttcccggg cgcctgcgcg
     7441 ccatccttgg cccaagccat ggctgtcctc cagccctgga atttttccgt actggatgtt
     7501 gaaaacctcc gaaatcacta cgtcaggact ctcgagttgt ggctcgaccg gttcgagagc
     7561 gcttgggaca gcgtcgtgga attactggga acggaa
//
LOCUS       DATNDT010000454         7456 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_415480, whole genome shotgun sequence.
ACCESSION   DATNDT010000454 DATNDT010000000
VERSION     DATNDT010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7456)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7456)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7456
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_415480"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..777
                     /locus_tag="VN788_14840"
     CDS             <1..777
                     /locus_tag="VN788_14840"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HXU21856.1"
                     /translation="IPMPRLSLWRRMQIPVFAWAAYWVIRLLGPTLRFEIVGVHNAVQ
                     IRDAGEPGIGAFWHRCILPAAWFWRNRGIVVLNTVNFDGQWTRRVIERLGFGTAQGSS
                     SRGAIEGLTAMAGSLEAGRHVGITIDGPRGPRYVAKPGPVILARRTGRPISVFHIALK
                     SAHTFQKSWDLFQFPYPFTDAVMFFAPPIRVPADADSDTMKQKQAEMQAALERVRDVA
                     ESWFGLTNGDRDRLREEWSEFYRPVPRTASVLTKPKPSAN"
     gene            complement(1283..2146)
                     /locus_tag="VN788_14845"
     CDS             complement(1283..2146)
                     /locus_tag="VN788_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HXU21857.1"
                     /translation="MKDFHRGLMKVLLPVCCILGLVGALFAQRPFHSYPSVEYGNSTP
                     LPPDWQRPGEWAFARLMYPPGPNDGYRGRFDGDWREGMSLWTQDGPPADREFAAAVRR
                     LTRVDARSVEQDVNLDDGNEVYNWPWLYAVQVGEWGITQHQADVLRDYLLRGGFFMAD
                     DFHGAYEWQMFLKRIRMVFPNRPIVDIPNNDPIFHTLYDLDDRYQIPGSEHLGRGYKV
                     PDYPQGPDDGKGARWLAIRDDKGRIMVAISFNSDLGDSWEWSNRADYPERFSDLGFRI
                     GINDLVYAMTH"
     gene            complement(2240..2860)
                     /gene="coaE"
                     /locus_tag="VN788_14850"
     CDS             complement(2240..2860)
                     /gene="coaE"
                     /locus_tag="VN788_14850"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HXU21858.1"
                     /translation="MLRAGLTGGIASGKSTVAALLRDREYQVLDADALAHELLEPGQD
                     SYKEVTSEFGLEILSPAGAIDRRKLGTIVFSNTEKRERLNQILHPRIHDVVVKWFEAL
                     DRPGGPEMAFEEAALILEAGAQRDLDCVIVCWCRPEQQFERLKERGFSQRDAELRIAA
                     QMPIDRKRKLADEVIDCSRSLEDTERQLVLLLEKLKKSPTCGRNTV"
     gene            complement(2860..3807)
                     /locus_tag="VN788_14855"
     CDS             complement(2860..3807)
                     /locus_tag="VN788_14855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HXU21859.1"
                     /translation="MAARILNGNAIRDQIYAELQAEIATLAAEGIRPGLAAVRVGDDP
                     ASKLYVNSKVAACERLGLASFYLTPPASTTTQELLAQVDELNRREDVDGILVQMPLPK
                     QIDSKKILDAVVPAKDVDGFHPVNVGNLVANRPALVACTPAGVMEILRRSDIPIEGAN
                     AVVIGRSDIVGKPMALLLLHANATVTICHSKSKDLPGIARRADIIVAAIGKAAMVTPD
                     FVRPGVTVIDVGQIVVRDAAEAQRIFAKFPTKLESFRTKGSILAGDVHPEVVEVAGAY
                     TPVPGGVGPLTIAMLMSNTVKAARMRRGTRVPAAASSHH"
     gene            4063..4674
                     /locus_tag="VN788_14860"
     CDS             4063..4674
                     /locus_tag="VN788_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HXU21860.1"
                     /translation="MIFGEGSERKQLERAMREAGRLPPGQSATLKWPVLHAGTVPHFD
                     PQKWDFQITGLVEKPVRITWAEFSRLPMKLVTADMHCVTRWSRFDVRWEGVPFTEVMK
                     LAVVKPEAKYVMVLAEQDYTSNVPLEDLMRSTTLFALKHNGEPLPVEHGYPVRLVVPH
                     LYAWKSVKWVRGIRFMEEDTPGFWEENGYNIYGDPFKEQRYSG"
     gene            4812..6539
                     /locus_tag="VN788_14865"
     CDS             4812..6539
                     /locus_tag="VN788_14865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU21861.1"
                     /translation="MIRLSALFASVLFAMAAPAFAQVQNYKPVTNEMLLNPSSNDWLM
                     YSRTYDARRDSPLKQINKKNVSQLRMAWVRGLGDGVTETIPIVHDGVMYVIAPGGFVD
                     ALDATNGDLIWEYKRPFKNPQTGSVERTKALAIYEDMVYFTAPDGYVVALDARTGKVR
                     WETFKTDTQNTSGALVVDGKVISGGTCGKGSESCFLEADNAETGEKVWRFHTVAQPGD
                     PGYASWNGDNSQSLASTWGLPGSFDAVRNVLYWGVANPMPDQRMTRHNGNPDGTSRTT
                     PADLYSNCTLALDPATGKLIWYYQHLPGDDWDTDHTHERTLAREAFNPNPKFVKWINP
                     DIPRGSMHDIAAMVSEGGAIFVLDRSNGQFLWATPFPYDDPNFAMSDINVKTGQTTLN
                     WNLVFKHPGDHHVICYWNTRSYWPTAFHQENNSLYVPYIDNCRDLTTAGPAGRGGWKV
                     IPRPGSDPNALTGLAKINLSTGEQMRFDVGRAPSNGAMLTTAGGLVFHGDMSRRFRAF
                     DVDTGKKLWEAIVGGNISVSTITYAVNGKQYVCVMTGFNLKVPELSKEVPNMHTPTYG
                     NAIYAFALP"
     gene            6628..7035
                     /locus_tag="VN788_14870"
     CDS             6628..7035
                     /locus_tag="VN788_14870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21862.1"
                     /translation="MKINRRKILSLLGISPAVLAATTPAIGEPRHSPKNPVLKDAKLE
                     VVNPKGLPPPIQLIPMAPRLPSLDGRTIFLVSDGFAGADRFLNQIAIWFQKNKPSVKT
                     VYRLKAGSYFDDDPKLMAEMKASGNAMIMAYGH"
     gene            7084..>7456
                     /locus_tag="VN788_14875"
     CDS             7084..>7456
                     /locus_tag="VN788_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXU21863.1"
                     /translation="MGLPTAPLVTIAFKDLAKTNAANRGMPGERICFLPHPMTNKTDA
                     EMYAVLGGNDPVTGKPLMPEIIDALTKPLTAEEEKSGKVTPDIGPATYVDTPENLQRI
                     YLDNNMTDFMPIILPTEEKVQA"
BASE COUNT         1660 a         2064 c         2167 g         1565 t
ORIGIN      
        1 attcccatgc cgcggctttc gctctggcga aggatgcaaa ttcccgtgtt cgcgtgggca
       61 gcgtactggg tgatccgctt gctgggtcca acgctgcgct ttgagattgt tggagtgcac
      121 aacgccgtgc aaattcgcga tgctggcgaa ccggggattg gtgcgttttg gcatcgctgt
      181 attcttcccg cggcatggtt ttggagaaat cgaggtatcg tggtgctcaa cactgtcaac
      241 tttgacggcc aatggactcg gcgtgtaatc gagcggctcg gattcgggac cgcgcaggga
      301 agttcgtcgc gcggcgcgat tgaaggcttg accgcgatgg caggaagcct cgaggcagga
      361 cgccacgtcg gaatcaccat tgacggtcct cgcggcccgc gctacgtggc gaagccgggt
      421 ccggtgattc tggcgcgccg aactggacgg cctatatccg tattccatat tgctctgaaa
      481 agtgcgcata catttcagaa gtcgtgggat ctttttcagt ttccatatcc gttcacagac
      541 gcggtgatgt ttttcgcgcc gccgattcgg gttcccgccg acgccgatag cgacacgatg
      601 aaacagaagc aggcggaaat gcaggcggct ctggagcgag tgcgcgatgt tgcggaatct
      661 tggtttggac tcacaaatgg cgaccgcgac cgccttcgcg aagagtggag cgaattctac
      721 cggcccgtgc cgcgaacggc gtcggtccta acgaaaccaa agccctccgc gaattaggca
      781 aatgtgatcc gtggtcttcg taacggcgga gcttttaagg gaagccatct ctttcgcctg
      841 tccctccgac cagtagtatt cccgcgcggc tttttctaag ctagtttgaa tggtgcagag
      901 agttcgacct ttcgtttgcg agcagtgtgc tagcgagcaa agcaattgga cggaaccgtg
      961 tcctcacgca atggaagttt ggaaggatgg atacaaccgc gctttgcggg agcgtccgcg
     1021 tcctcggctg acgtctgagg acgtacgtaa ggcaatagac ggattatcgt cctcttggaa
     1081 gaacttttac cacctggccg ccgacgcact caatcgcata ctcgatgaga aagaaaaaaa
     1141 accgtaagcc gctcttcttc cgagtccgac aaccgcataa agcggcctgc caccgaacga
     1201 tgcatcacgt accttcgcta gaacgggctc tattcgtcgc ttcgtaagat cagattgtca
     1261 gagcggattc ccctgcaagg ggtcagtgtg tcattgcgta gacgagatcg ttgatcccga
     1321 tgcggaaacc gagatcggaa aaacgctcgg ggtaatcagc gcgattcgac cactcccatg
     1381 aatccccgag atcggaattg aaagatatgg cgaccatgat acggccctta tcatcgcgga
     1441 tagcgagcca ccgcgcaccc tttccatcgt ccgggccctg tggataatcc ggtactttgt
     1501 agccccttcc taaatgctcg gagccgggaa tctggtagcg gtcgtcgaga tcgtataagg
     1561 tgtgaaatat cgggtcgtta ttcgggatgt cgacgattgg gcgattcggg aaaaccattc
     1621 tgatgcgctt caaaaacatc tgccattcgt acgcgccatg aaaatcgtcg gccatgaaga
     1681 atccgccgcg cagtagatag tcacgcaaga cgtcggcttg atgttgagtg atcccccatt
     1741 cgcccacctg cacagcataa agccacggcc aattgtagac ttcgttccca tcatcgagat
     1801 tcacgtcctg ctccaccgat cgggcgtcga cgcgcgtgag cctccggacc gcagcggcaa
     1861 attcgcgatc ggctggcgga ccatcctggg tccacagcga catcccttcg cgccagtcac
     1921 catcgaaacg cccacggtaa ccatcattgg ggccgggtgg atacatgaga cgcgcgaaag
     1981 cccattcgcc gggccgctgc caatccggtg gcagcggagt ggaattgcca tattcgacag
     2041 agggatatga atggaaaggg cgctgagcaa acagcgcgcc tacaaggcca aggatgcagc
     2101 aaaccggcaa aagaactttc atcaagcccc gatggaagtc tttcatggca gcttcgcatc
     2161 tccttggccg gcggcgctcg cttgtagtct acgattctat cgcgataatt cgtagggcgg
     2221 gttccaagta gatgcgaatt tagacggtat tccttccgca ggtgggcgat tttttcagtt
     2281 tttcgagaag caggacgagt tggcgttcgg tatcttcgag ggagcgcgag cagtcgatca
     2341 cctcgtcggc gagtttgcgc tttcggtcga tgggcatctg cgcggcgatg cggagttccg
     2401 cgtcgcgctg cgagaatccg cgttccttca aacgttcgaa ctgctgctcg gggcggcacc
     2461 agcagacgat tacgcaatcg agatcccgct gcgcaccggc ttcgagaata agcgccgctt
     2521 cctcgaaggc catctcgggg ccgcctggac ggtcgagcgc ttcaaaccac ttcaccacaa
     2581 cgtcgtggat gcgcgggtga agaatctggt tcaggcgctc gcgcttttca gtattggaaa
     2641 aaacgatggt gccgagtttc cggcggtcga tggcgcccgc tggagagagg atttccaaac
     2701 cgaattcgct tgtaacttct ttgtaggaat cttgcccggg ctcgagcaat tcgtgcgcca
     2761 gcgcgtcggc atcgagcacc tgatattcgc gatcgcggag gagagcagct acagttgact
     2821 tgcccgatgc gattccgccg gtgagccctg cgcgcagcat cagtggtgcg aggaagcggc
     2881 cgccgggacg cgcgtcccgc gccgcatgcg agcggctttc accgtgttgc tcatcagcat
     2941 cgcaatggtg agcggaccta cgcctccggg cacgggcgtg taagcgcccg cgacttcgac
     3001 gacctccgga tgaacgtcac ccgcgaggat ggaacctttc gtacgaaacg attcgagctt
     3061 cgtggggaac ttggcaaaga ttcgctgggc ttcggctgcg tcccttacca cgatctggcc
     3121 gacgtcaatc acggtgacgc cagggcgcac aaaatccggc gtcaccatag cggcttttcc
     3181 gatggctgcg acaatgatat ccgcgcggcg tgcgatgccc ggcaggtcct tgcttttcga
     3241 atggcagatt gtgacggttg cgttggcgtg aagcagaagg agagccatcg gtttgcctac
     3301 gatatcgctg cgtccgatca ccacggcgtt cgcgccttcg atcggaatat cgctgcggcg
     3361 gagaatttcc atcacgccag caggagtgca ggctacaagc gccgggcggt tagcgaccaa
     3421 attgccgaca ttgacggggt gaaaaccgtc cacgtctttt gcgggaacaa cggcgtcgag
     3481 aattttcttg gagtcaatct gcttaggcag gggcatctgt acgaggattc cgtccacatc
     3541 ctccctgcga ttcaattcat cgacttgagc aagcaattct tgagtggtcg tcgaagcggg
     3601 cggcgtgaga tagaaactgg ccaggccgag cctttcgcac gcggcgacct tgctattcac
     3661 atagagcttc gatgcgggat cgtcgcccac gcgcaccgca gcaagtcctg ggcgaattcc
     3721 ttccgccgcc agcgtggcga tttccgcctg caactcagcg tagatttgat ctcgaatcgc
     3781 gttgccattg aggatgcgtg cggccaatgg aagctcccgg ttgtgatttt ttctatgttc
     3841 gaatctcgca tcatagcata cgcaatttcc gccagatgcc gggattggtt caatgtaggg
     3901 aagctgttcg tgtccgccca agcgccggag aaatcatgtg ggcgcactac gaggatacga
     3961 gaaatatctt tacgcagagg cccgatatga tgcaagtact tgttggtgtg aagaaaaatc
     4021 gtccatggta ccggtgacag acatggccaa aatgagggcg cgatgatctt cggcgaaggc
     4081 tcggagcgga aacaattgga acgggcgatg cgcgaggccg ggcggttgcc acccggccag
     4141 tcggctacgc tgaagtggcc ggtgcttcat gccggcacgg tgccgcactt tgacccgcag
     4201 aagtgggatt ttcagatcac cgggctggtt gaaaagccgg tgcgaattac gtgggcggaa
     4261 ttttcccgcc ttccaatgaa gctagttaca gccgacatgc attgtgtcac gcgatggagc
     4321 cggttcgatg tccgctggga aggggtgccg ttcaccgaag tgatgaagct ggcggtggtg
     4381 aagcccgaag caaaatatgt gatggtgctc gcggaacaag actatacgtc caatgtgccg
     4441 ctcgaggatc tgatgcggtc aacgaccctc ttcgcgctga aacacaatgg cgagccgctg
     4501 cctgtggagc acggctatcc ggtgaggctg gtggtgccgc atctgtatgc ttggaaaagc
     4561 gtgaagtggg tgcgcggcat tcgattcatg gaagaggata cgccgggctt ctgggaagaa
     4621 aatggctaca acatctacgg cgacccattc aaagaacaaa ggtatagcgg ttagaaacgc
     4681 gcaaggttgg atccttcatc attagaaccc acttgaattg aatcaatctc tagaaacttt
     4741 cttcttgtgc gcgggactaa catcgacgat atatttctcc gtacttttat tttatttgac
     4801 aagggagaaa tatgatcagg ctttcggccc tctttgcgtc tgttcttttt gccatggcag
     4861 cgcccgcctt cgcgcaggtc caaaactata agccggtaac caacgaaatg cttctaaatc
     4921 caagttcgaa cgactggctg atgtacagcc gcacatacga cgcgcgacgt gatagccctc
     4981 tgaagcagat caacaaaaag aacgttagcc agcttcgcat ggcttgggtg cgcggcctcg
     5041 gcgacggcgt caccgaaacg attccgatcg tgcatgatgg cgtgatgtac gtgatcgcac
     5101 ccggtggatt cgttgatgcc ctcgacgcca ccaacggcga tctgatttgg gagtacaagc
     5161 ggccattcaa aaatccgcaa accggttcgg tggaaagaac caaggccctg gcgatctatg
     5221 aagatatggt ttatttcacg gcgcccgatg gctatgtggt ggcgctcgat gcgcgcacgg
     5281 ggaaggtccg ctgggaaacg ttcaagacgg acacgcaaaa tacctcgggc gcgctggtgg
     5341 tcgatggcaa agtgatctcg gggggaacct gcggcaaggg cagtgaaagt tgctttctcg
     5401 aagccgacaa tgccgaaacc ggcgaaaaag tttggagatt ccacaccgtt gctcagcccg
     5461 gcgatccggg ctatgcatcg tggaatggcg ataattcgca atcgctggcg tccacatggg
     5521 gcttgccagg aagcttcgac gctgttcgca acgtgctcta ctggggcgtc gcgaatccca
     5581 tgccggacca gcgcatgact cgccacaacg gcaatcccga tgggacctcg cgcaccactc
     5641 cggccgatct ctatagcaac tgcaccttgg ctctcgatcc cgcgacggga aagctcattt
     5701 ggtattacca acaccttccg ggcgacgatt gggacacgga ccacacgcat gaacgcacgc
     5761 tggcgcggga ggcgttcaat ccgaatccta aattcgtgaa gtggatcaac ccggacattc
     5821 cgcgtggatc gatgcacgat attgcggcca tggtttcgga gggcggcgcg atttttgtcc
     5881 tcgaccgcag caacggccag tttctatggg ccacgccatt tccctacgac gatccgaatt
     5941 ttgcgatgtc cgatatcaac gtgaagacgg gacaaaccac gctcaactgg aatctcgttt
     6001 tcaagcatcc aggcgatcac cacgtaattt gttattggaa tacgcggagc tactggccca
     6061 cggctttcca tcaggaaaac aattcgctgt acgtgcccta tattgataac tgtcgagatc
     6121 tgacgactgc cggacccgcg gggcgcggtg gctggaaggt aattcctcgt cccggttccg
     6181 atcccaatgc gcttaccgga cttgccaaga tcaatttgtc caccggcgag cagatgcgct
     6241 tcgatgtggg ccgcgcgcca agcaacggcg ccatgctgac cacggcgggc ggactagttt
     6301 tccatggaga catgtcgcga agatttcgcg cgttcgatgt ggacaccggc aaaaagttgt
     6361 gggaagcaat cgttggcgga aatatctccg tgagcacgat cacctacgcg gtgaacggca
     6421 aacagtacgt ttgcgtgatg acgggtttca acttgaaagt gccggagctg agcaaagaag
     6481 tgccgaatat gcatactccg acgtacggta acgcaattta cgcgtttgcg ctgccctagg
     6541 actctgcggc ggcaaatggt agtaatatgt aacccttatg attaacgaac ggtctattaa
     6601 tcgtaggccc ggaagcgagg ataaaccgtg aagatcaata ggcgcaaaat cctgtctctg
     6661 ctcggaattt ctcccgccgt tttggcagca acaactcctg ccatcggcga accaaggcat
     6721 tcgcccaaaa atcccgtcct caaggacgcg aaattggaag tcgtaaatcc caaggggttg
     6781 ccgcctccga ttcagctcat tcccatggcg cctcgccttc cgagccttga cggtaggacg
     6841 attttcttag tgtctgacgg cttcgccggc gccgatcgct tcctgaacca gattgccatc
     6901 tggttccaga aaaataagcc gagcgtgaaa acggtgtacc gcctgaaagc tgggtcgtat
     6961 ttcgacgatg accccaagct aatggcggaa atgaaagcca gcggcaacgc catgatcatg
     7021 gcgtatggcc attgaagcac ctgcacacca gcgaccgtcg gtcactgcgc acaagttgag
     7081 aagatggggc tccccaccgc gcccctggtt acgatcgcct tcaaggatct cgccaagacc
     7141 aacgccgcga accgcggcat gcccggcgag cgcatctgct tcctcccgca cccgatgacc
     7201 aacaagacgg acgcggaaat gtacgccgtt ctcggtggca acgaccccgt caccggcaag
     7261 ccgctgatgc cggaaataat agacgcgctc accaagccgc tcacggccga agaggaaaag
     7321 agcggcaagg taactccgga catcggcccg gcgacctacg tggacacgcc agagaatctg
     7381 cagcggatct acctcgataa caacatgacc gacttcatgc ccatcatcct tcccacagaa
     7441 gaaaaggtgc aagcga
//
LOCUS       DATNDT010000455         3688 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_111057, whole genome shotgun sequence.
ACCESSION   DATNDT010000455 DATNDT010000000
VERSION     DATNDT010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3688
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111057"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(609..1235)
                     /locus_tag="VN788_14880"
     CDS             complement(609..1235)
                     /locus_tag="VN788_14880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21864.1"
                     /translation="MKIFGRKAAADAGRARAARQKEDQLSSSHRVTLDLYRAEVLAAM
                     MAKSRSSESIETTDLLAGMYISNWERLSRYWPNSKQDEIEILLRRICRISPQRWHSWI
                     ESYQTNRSDTDKRVWQRLRRSKNGKAADNSLSPSADMLSVLKRAERITPFHERSGATS
                     TPILSMECVLLCILRNSGAEISRRLAATGIDETNLERDVLLPRRGPRT"
     gene            1507..2466
                     /locus_tag="VN788_14885"
     CDS             1507..2466
                     /locus_tag="VN788_14885"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HXU21865.1"
                     /translation="MALREGILSRRNVFSLLFGFVLAAGLVSSAPAASAQQTWHIQVG
                     VQSQAERADAFLPNEIWIYAGDSIKFTFSSKNEAHTVTLLESGQARPLFSSPMANEVA
                     YIVKFPKPGNYKLAHLAHSDMYGVVHVLQKTDSAAAFYAASLPYDQFDYDHQAAEEMS
                     ELLAGENDPSEEKRDFAPSENVVLLTGEIVVDAEGGQYLANVHFLPATIQIHAGETVE
                     WINTDPMVPHTVTFGPQPVNPGTLVTAARGPDGALQATINSPADSVSSGFLEAAAQNR
                     PFQPQSAPAGARLRIKFTKPGTYHCICDPRDTHGMVGTVIVLP"
     gene            complement(2552..>3688)
                     /gene="glnA"
                     /locus_tag="VN788_14890"
     CDS             complement(2552..>3688)
                     /gene="glnA"
                     /locus_tag="VN788_14890"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HXU21866.1"
                     /translation="QDPLSHKPYDRDVRNLAHRAEEYLQKTGIADQAFFGAEAEFFIF
                     DNVRFDSRENHAYYYVDSDEGRWNSGRADQPNMGFRPRYKEGYFPVPPTDRYQDMRSE
                     MVLKLQEVGIEVECHHHEVATAGQCEIDFKFDTLIRAADNMCKYKYVLRNTGYKHGKT
                     VTFMPKPVFSDNGSGMHTHQSLWNKGKPLFAGDGYAGLSQTALWYIGGLLKHAAAVVG
                     FAAPSTNSYKRLVPGFEAPVNLAYSRRNRSAAVRIPMYSTNPKAKRLEFRPPDPSCNP
                     YLAYSAMLMAGLDGIENKIDPGGPLDKDIFDLSKEELSKVPSVPGSLEESLKALDQDH
                     AFLTKGGVFTKEFIDLWIDYKMSKEVNPMRMRPHPYEFHLYYDI"
BASE COUNT          805 a          979 c         1139 g          765 t
ORIGIN      
        1 cgactgcgtc tgccggcatc cgtcgcagag aacgcgacgg atggaaagga aaaccggcag
       61 actccgctca gaatgacgct ggatcgggcc ggaactcagg ggagttgagg tgatgagaat
      121 aaatgggcgg agtacgagga tacagaggaa atctttgcat acggggtcga aatcgcgcaa
      181 gtcgttgttg gtgtaaagaa aaatcgtcca cggtgagtgt ggacttttgg tgttttgtac
      241 gccggtcgaa agagtcgtga ttctggcgca cgcgcggagc acgctcgggc cagtccgagc
      301 ggcaaacagc gggagcttgc tcccgcactc cacatactgc gcggcggatg gtgagcatca
      361 cgccaagtat gtttgccggg cacggcatgc tgtgccccta ccaatgcaaa ggcaaaaggg
      421 cgcagcaacc ttacgcggaa accgctccgg gcaaggccgc cccctacaaa ggcaagggcg
      481 aaagcaaaag caggccccta caaacgcaaa gtcaaagggc gcaggcaact ctacgcagaa
      541 accactcagg gcaagaccgc gcccctacaa aggcaaaagc aaaaacagcg ccgtggctct
      601 ccaacttgtc aggtgcgggg gccgcggcgg gggaggagga cgtctcgctc gaggttcgtc
      661 tcgtcaattc cggtggcggc gagcctgcgg ctgatttcgg cgccggagtt tcgcaggatg
      721 caaagaagaa cacattccat gctcaggatg ggggtcgaag tagcgcccga tctttcgtgg
      781 aacggggtga tgcgttccgc cctcttcagc acagacagca tatccgcgga aggactgaga
      841 gaattgtcgg cggctttgcc atttttcgat cttcgcagcc tttgccaaac tcttttgtcg
      901 gtatcgctgc gattcgtttg atatgactcg atccaggagt gccatctctg ggggctgatg
      961 cggcagattc tccgcaaaag tatctcgatt tcgtcctgct tgctgttcgg ccaatacctg
     1021 gagaggcgct cccaattgga aatgtacatt ccggccagca ggtcggtggt ttcgatggat
     1081 tccgaggagc ggcttttggc catcatcgcg gccagaacct cggcgcgata gagatcaagg
     1141 gtgacccgat gcgatgagga gagttgatcc tctttttgcc tggcagcgcg tgcgcgaccg
     1201 gcgtccgcgg cggctttgcg accgaagatt ttcacgatga acgaagcccc ctcaaagaca
     1261 gtctatcagc gcaggcgcgg acatcgcggc ttctctgcta tccggttgac aatttgatta
     1321 atcgcggcga aacggggaac gtccgaacgt ggatgcgttg tgaaggccga aacggcccag
     1381 tgggtcgaat gatactctca tatcccacga agatttcact cggcagttgc taacacgagg
     1441 cgtttgggtt acacgatgac cagctagatt ccaactggga atgctggctc tcagggaggc
     1501 tcggcaatgg cgttgcgcga agggattttg tcgcgccgaa atgttttttc gttgcttttt
     1561 ggtttcgtgc tcgctgccgg gcttgtgtcg tccgcgccgg ccgccagtgc gcagcagacg
     1621 tggcatatac aggtcggcgt gcagagccag gcagagcggg ccgacgcgtt tttgccgaat
     1681 gagatctgga tctacgcggg cgacagcatc aagtttacgt tttcttccaa gaacgaggcc
     1741 cacacggtga ctctcctgga aagcgggcag gcgcgtccac ttttttcgag tccaatggcg
     1801 aatgaggtgg cctacatcgt gaaattccca aagcccggga actataaact ggcacacctg
     1861 gctcactccg acatgtatgg agttgtccat gtcctccaga agacggattc cgcggcggcg
     1921 ttttacgcgg catctctacc ctacgatcag tttgactatg accatcaggc cgcggaggag
     1981 atgagcgaac tactcgccgg cgaaaatgat ccttcggaag agaaacgcga tttcgcgcct
     2041 tctgaaaatg tcgtcctgct gacgggagag atcgtcgtcg atgctgaagg agggcagtat
     2101 ttggcgaatg ttcactttct gccggcgacg atccaaatcc atgccggaga aactgtggaa
     2161 tggatcaata cagatccgat ggtgccgcat acggtcacgt tcggacctca gcccgtgaat
     2221 cccgggactc tagtgacggc ggcgcgcgga cccgatggag cgctgcaggc gacgatcaac
     2281 tcgcccgcgg atagcgtgag ctccggtttc ctggaggcgg cagcgcagaa tcgccccttt
     2341 caaccgcaat cggcgccagc aggagcgcgc ctccggatca aatttacgaa gcctggcact
     2401 taccactgca tctgcgaccc acgcgatacg catggaatgg tgggaacggt aattgttcta
     2461 ccataagcga atggaaaggc agatccctcg gtcgagttgc gcactccctt cctgcgtcag
     2521 ggcgagcggg atagcgatcc cgctcgtagc gctagatgtc gtagtacaag tggaactcat
     2581 acgggtgcgg acgcatccgc atgggattca cttccttcga catcttgtaa tcgatccaga
     2641 ggtcgatgaa ttcttttgtg aacacgccgc ccttggtgag gaaggcatga tcctggtcga
     2701 gagccttgag agactcctct agcgagccag gaaccgacgg aactttcgag agttcttcct
     2761 tggaaagatc aaagatatct ttgtcgagcg ggcctcccgg atcgattttg ttctcgatgc
     2821 cgtcgagccc ggccatgagc atcgccgaat aagcgagata gggattgcac gacggatcgg
     2881 gcgggcggaa ctcgagccgc ttggccttgg gattcgtcga atacatcggt atgcggacgg
     2941 cggcggagcg attgcggcgc gagtaagcga ggttcacagg cgcctcaaag ccggggacga
     3001 gccgcttgta agaatttgta gagggcgccg caaaaccaac cacagccgca gcatgtttga
     3061 gaagtccacc gatataccac agcgcggtct ggctcaagcc cgcgtagcca tcgccggcga
     3121 agagtggctt gcctttattc catagcgatt ggtgagtgtg catgcccgaa ccgttgtcag
     3181 aaaagacagg cttgggcatg aaggtgacgg tcttgccgtg cttgtagccg gtattgcgca
     3241 ggacgtactt gtacttgcac atgttgtccg cggcgcgaat cagcgtgtcg aatttaaagt
     3301 caatttcgca ctggccggcg gtggcgactt cgtggtggtg acactcgacc tcgatgccaa
     3361 cttcttgcag cttcaaaacc atctcgctgc gcatatcctg atagcgatcg gtgggaggta
     3421 cggggaagta accttccttg tagcgcgggc ggaagcccat gttgggctga tcggcacggc
     3481 cggaattcca gcgcccttca tccgagtcca cgtagtagta ggcgtgattt tcgcgggagt
     3541 cgaagcgaac gttatcgaaa ataaaaaact cggcctcggc gccgaagaag gcttggtcgg
     3601 cgatgccggt cttctgaaga tattcctcgg cgcgatgagc gaggtttcgc acgtcgcgat
     3661 cgtaaggctt gtgcgagaga ggatcttg
//
LOCUS       DATNDT010000456        10850 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1827206, whole genome shotgun sequence.
ACCESSION   DATNDT010000456 DATNDT010000000
VERSION     DATNDT010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 10850)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10850)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10850
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1827206"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /gene="infA"
                     /locus_tag="VN788_14895"
     CDS             complement(<1..191)
                     /gene="infA"
                     /locus_tag="VN788_14895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013406388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HXU21867.1"
                     /translation="MPRKPLQEKKKPGEGSGKEDAIEVMATVIEPLPNAMFRVELENK
                     HQVLAHISGKMRKNFIRILA"
     gene            complement(221..994)
                     /gene="map"
                     /locus_tag="VN788_14900"
     CDS             complement(221..994)
                     /gene="map"
                     /locus_tag="VN788_14900"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HXU21868.1"
                     /translation="MVICKSSAELEKMHQAGLLVWGALEKMREMVRPGLSTKELDEFA
                     ETYTAEHRARPAFKGYRGYPGSVCTSINQEVVHGIPSSSRRVREGDILSMDFGVELSG
                     YFADAALTVPVGKIPPQREKLMRVTRESLDRAISKVRVGNRLGDVSAAVQEWVEENGF
                     SVVREFVGHGIGTHMHEEPQVPNYGAPGHGPRLQEGMVIAIEPMVNSGGPGVRVLDDD
                     WTAVTADGSDSAHFEHTVAVTNNGPWILTRPKDATGPCW"
     gene            complement(981..1661)
                     /locus_tag="VN788_14905"
     CDS             complement(981..1661)
                     /locus_tag="VN788_14905"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HXU21869.1"
                     /translation="MPLNRALIFLGPPGAGKGTQSKRIAQRYGVPHLSTGDMFRDAIN
                     RGTPLGQIVRPIMERGDLVPDDLVMRMVEERLSQPDSAKGFVFDGFPRTLQQAEQLDR
                     ILERKGFGKPIVVDFKVEDGELFRRLSGRWTCSVGGEIYNVYDAPPRTPGICDTDGGV
                     LVQRPDDRPEVVKERLAAYERQTKPLADYYRRQGLLDTVNAAANVEDVGRALGEIFRR
                     AEGRDGHL"
     gene            complement(1692..3101)
                     /gene="secY"
                     /locus_tag="VN788_14910"
     CDS             complement(1692..3101)
                     /gene="secY"
                     /locus_tag="VN788_14910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HXU21870.1"
                     /translation="MRKLVEAFRNIFSTPDLRKRVLFTLGVLGVYRLGAFIPTPGINT
                     DLLQQLFEQNKGSVLGIIDLFSGGNFRRLTIFALGIMPYITASIILQLLVVVWPYLER
                     LQKEGELGRRKITQYTRYLTIILSVFQSFAIARTLTAQGLAGQSLVYHPGYRFMLMTI
                     LTLTTGSAFIMWLGEQISDRGIGNGMSLIIFAGIVVGLPRAIQDLWQKVRTEQWGSFT
                     PITIIALLIFMIAVVAFIVFVEGGQKRVPVQYAKRVMGRKVMGGQMTYLPLRVNSGGV
                     MPPIFASSLLTFPQTLALIFQSRSPAIQALGKMIGWGEPLYTLLYVVLIVFFAFFYVG
                     IIFNPTELADNMRKYGGFVPGIRPGRNTSEHINRILTRLTFVGGVYLAVVCLLPEWMI
                     SGIHLQHLPTWLGGNWFDTHTWRFILDGLGVQFYFGGTSLLIVVGVAMDTVQQLEAQL
                     VMRNYEGFVRKGRVRSRRG"
     gene            complement(3098..3553)
                     /gene="rplO"
                     /locus_tag="VN788_14915"
     CDS             complement(3098..3553)
                     /gene="rplO"
                     /locus_tag="VN788_14915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HXU21871.1"
                     /translation="MANVPTLSNLKPPRGSRHRKVRVGRGMGSKLGKTSGAGNKGQQS
                     RRGYSRRPGFEGGQMPLHRRLPKRGFSAPFSKTFAVVNVENLNAFAAGDTVTPESLTE
                     RGIVRASRSGIKVLGNGELKVALTVRAHAFSKSAQEKIAGAGGKTEVLS"
     gene            complement(3559..3756)
                     /gene="rpmD"
                     /locus_tag="VN788_14920"
     CDS             complement(3559..3756)
                     /gene="rpmD"
                     /locus_tag="VN788_14920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041570711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HXU21872.1"
                     /translation="MATKKKESGTLKIKWVRSFIGCPRGMRQTIRGLGFRRMQQVVVR
                     QDTPETRGMIAKVHHLVEIEE"
     gene            complement(3774..4322)
                     /gene="rpsE"
                     /locus_tag="VN788_14925"
     CDS             complement(3774..4322)
                     /gene="rpsE"
                     /locus_tag="VN788_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HXU21873.1"
                     /translation="MPKQELKDTLSIRRTVQTNQANLKDQVVSINRVTKVVKGGKNLS
                     FSALVVVGDQGGHAGFGMGKAREVPMAIRKAIEQAKKNMVKLNLKGSTIPHQVLGSYG
                     SGQVLLKPAPEGTGVIAGGPVRAVMEVAGIQNVLTKSLGTSNPHNVIKATFDALLHLK
                     DAARVAETRGKSAEEAPVATGQ"
     gene            complement(4328..4705)
                     /gene="rplR"
                     /locus_tag="VN788_14930"
     CDS             complement(4328..4705)
                     /gene="rplR"
                     /locus_tag="VN788_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HXU21874.1"
                     /translation="MAAPVRKLSREAHRRRIHVRTRKRLEGTMERPRLCVHRSSRHIR
                     AQVVDDGAGRTLVSASSLDKEVRAAIKSGGNIAASKVVGKIVADRARAKGIEQVVFDR
                     GGYQYHGRVQALAEAAREAGLKF"
     gene            complement(4730..5272)
                     /gene="rplF"
                     /locus_tag="VN788_14935"
     CDS             complement(4730..5272)
                     /gene="rplF"
                     /locus_tag="VN788_14935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HXU21875.1"
                     /translation="MSRIGNKLIAVPSGVKIQIKADAVEVQGPKGKMTVGLPRGIQFE
                     QKDGTLVAKREAEEFRALHGLARALVANAVMGVTQGFKKDLDIVGVGYRAEVKGKNVV
                     LALGYSHPIEFPIPEGIQIAVEKQTHLTVTGADKNQVGQVAANLRRLRPPDPYKQKGV
                     RITGERLKKKAGKAGAKTAA"
     gene            complement(5291..5686)
                     /gene="rpsH"
                     /locus_tag="VN788_14940"
     CDS             complement(5291..5686)
                     /gene="rpsH"
                     /locus_tag="VN788_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HXU21876.1"
                     /translation="MTTDPIADFLTRIRNGSRARHSRVDMPSSKMKIEIARILKDEGY
                     VVNFKVVEEKGKKTLRVFLRYTPERRSVITDLKRVSRPGSRRYVGKFGIRPVVGGMGI
                     AIVSTPRGLMTGQAARKEGVGGELLCEVW"
     gene            complement(5699..5884)
                     /locus_tag="VN788_14945"
     CDS             complement(5699..5884)
                     /locus_tag="VN788_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HXU21877.1"
                     /translation="MARTSQIAKTQRKLKYKIRQRNRCRICGRARGTLRKFQMCRICF
                     RGLALKGEIPGVVKASW"
     gene            complement(5900..6454)
                     /gene="rplE"
                     /locus_tag="VN788_14950"
     CDS             complement(5900..6454)
                     /gene="rplE"
                     /locus_tag="VN788_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014042523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HXU21878.1"
                     /translation="MSSRLHEKYRKDAVPALTKRFGYKNTMAVPRVSKVTLNIGLGEA
                     SQNVKLLDTAAVELGQIAGQKPVITRAKKSIANFKIRKGMPIGCMVTLRGERMYEFLD
                     RLSAIVFPRVRDFKGLAPNSFDGRGNYTLGLKDQLVFPEIDYTRVDKIKGMNITITTT
                     ARSDEEGRELLKLLGVPFRAQGQA"
     gene            complement(6470..6817)
                     /gene="rplX"
                     /locus_tag="VN788_14955"
     CDS             complement(6470..6817)
                     /gene="rplX"
                     /locus_tag="VN788_14955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HXU21879.1"
                     /translation="MRKIAEKPAPLGIRHGDTVKVMAGRSKGMTGKVLSVNLEKSRVT
                     VEHANIIKRHTRPNPSKNIRGGILDKEGPIHISNVMLVCPGCGKHTRAGHTALPDGTK
                     VRVCRRCNTTFEN"
     gene            complement(6821..7189)
                     /gene="rplN"
                     /locus_tag="VN788_14960"
     CDS             complement(6821..7189)
                     /gene="rplN"
                     /locus_tag="VN788_14960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HXU21880.1"
                     /translation="MVQMRTWLTVADNSGARQITCILPIGGDAGLTAGLGDVITAAVK
                     EATPDGQVKKGKVVRAVVVRTRKEQRRKDGSYIRFDDNAAVLINDANEPIGTRVFGPV
                     ARELRDKKFTKIVSLAPEVW"
     gene            complement(7226..7540)
                     /gene="rpsQ"
                     /locus_tag="VN788_14965"
     CDS             complement(7226..7540)
                     /gene="rpsQ"
                     /locus_tag="VN788_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HXU21881.1"
                     /translation="MANETMAPDTPKSGQGVRERGIRQELVGVVTSAKMQKTIVVKVT
                     RATQHPLYQRVVRSGKKYYAHDETGEARVGDIVRIAATRPLSKLKRWRLKEVIQRSTR
                     AA"
     gene            complement(7540..7743)
                     /gene="rpmC"
                     /locus_tag="VN788_14970"
     CDS             complement(7540..7743)
                     /gene="rpmC"
                     /locus_tag="VN788_14970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019433422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HXU21882.1"
                     /translation="MARSVEKYREMNSDELTVQERELAEQIFRLRFQIAAGQAEGLKK
                     LREAKKDFARVKTLLREVELRKA"
     gene            complement(7743..8159)
                     /gene="rplP"
                     /locus_tag="VN788_14975"
     CDS             complement(7743..8159)
                     /gene="rplP"
                     /locus_tag="VN788_14975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015223454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HXU21883.1"
                     /translation="MLMPKKVKFRKQQRGRRTGKAWRGGSLAFGEYGLKALEPAWITD
                     RQIEAARVAITRFIKRGGKLWIRIFPDKPFTKKPAETRMGKGKGPPEHWVAPVKPGRI
                     MFEMAGVDLASATEAMELAAHKLPIATKFVTRSEEL"
     gene            complement(8180..8842)
                     /gene="rpsC"
                     /locus_tag="VN788_14980"
     CDS             complement(8180..8842)
                     /gene="rpsC"
                     /locus_tag="VN788_14980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HXU21884.1"
                     /translation="MGQKTHPYGFRLGYNKGWRSRWFAKKDYADLLHEDVKLRTQLKD
                     RLRSAGINGIDIERAANKLVVRIYTARPGIIIGRKGSEIDKLKAEVQKRTNREVHIDI
                     QEVHRPELDATLVAESIALQLEKRVAFRRAMRKAVDSALRFGCKGIKVRVAGRLNGAE
                     IARKEWYLQGRLPLQTLRADIDFGTAEAHTTYGLIGVKCWVYQGEKVPLRSKPAAAGA
                     LI"
     gene            complement(8858..9361)
                     /gene="rplV"
                     /locus_tag="VN788_14985"
     CDS             complement(8858..9361)
                     /gene="rplV"
                     /locus_tag="VN788_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014263812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HXU21885.1"
                     /translation="MMATLAQSQIEGMANTRFVRVSPQKARLVIDLIRGRRAEDALQT
                     LRFTKKRVAKEIEKTLRSAIANAERKADDAGESLDVDALYVTGCCVNEGPRWKRMRPA
                     PMGRGFRYQKRTSHIVVQVAEHHRAAADRAAAAVAEAEASKGVRGAVRKARKAIEKRA
                     KEAQKKK"
     gene            complement(9377..9724)
                     /gene="rpsS"
                     /locus_tag="VN788_14990"
     CDS             complement(9377..9724)
                     /gene="rpsS"
                     /locus_tag="VN788_14990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HXU21886.1"
                     /translation="MPRSLKKGPFIDTHLQEKVAALNARNEKKVVKTWSRRSTILPEM
                     VGHTIAVHNGKKFIPVYITEQMVGHKLGEFAPTRTFKGHGVKVALERAAAAAAGPGGA
                     AAPAGGAPPAGKS"
     gene            complement(9732..9980)
                     /locus_tag="VN788_14995"
     CDS             complement(9732..9980)
                     /locus_tag="VN788_14995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21887.1"
                     /translation="MKPVAKPAIKPVGKVSKLGPAPQVLFRLCRAGWAVIVDKIPNAQ
                     GTRFVCPFCPLSHRVPGPVRGFRKMRRAQWVKLRRVEE"
     gene            complement(10126..>10850)
                     /gene="rplB"
                     /locus_tag="VN788_15000"
     CDS             complement(10126..>10850)
                     /gene="rplB"
                     /locus_tag="VN788_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HXU21888.1"
                     /translation="TEAKKRTGGRNAHGEVTSWHRGGGHARRFRSIDFRREKIGIPAK
                     VAAIEYDPNRSARIALLHYADGEKRYILQPVGLEVGMTVQNGEGADILPGNALTIRHI
                     PPGTMVHNLELFPGKGAQVVRAAGGAAQLLSKEGDFALVKLPSGEVRKFSVDCMATVG
                     QLGNLDHENVTYGKAGRTRWHGKRPTVRGVAMNPVDHPHGGGEGRVKGNHPQTPWGFP
                     TLGAKTRKKRKSDRMIVERRKP"
BASE COUNT         2060 a         3414 c         2966 g         2410 t
ORIGIN      
        1 gcgagaatgc ggataaaatt cttgcgcatc ttgccggaga tatgggcgag cacctgatgc
       61 ttgttttcca gctcgacccg aaacatggca ttggggagcg gctcgatcac cgtggccatg
      121 acctcaatcg cgtcttcctt cccacttccc tcgcccggct tcttcttttc ctgcagcggc
      181 tttctcggca tgcgctccct ttaaatccta gtcgtcgcga tcaccagcaa ggcccagttg
      241 cgtccttcgg gcgcgtcaaa atccacggcc cgttattggt tacggcgacg gtatgctcga
      301 aatgagccga atcgctgccg tcggcggtga ccgcggtcca gtcgtcatca aggacgcgga
      361 cgccggggcc gcccgaattc accataggct cgatggcgat gaccatgcct tcctgaagcc
      421 gcgggccatg accgggcgca ccgtaattcg ggacctgcgg ttcctcgtgc atgtgggttc
      481 ctatgccgtg acccacgaac tcgcggacca ccgaaaagcc attttcctca acccattcct
      541 ggactgccgc cgaaacatcg ccaaggcgat tgccgacgcg caccttgctg atcgcgcgat
      601 cgagcgattc tcgcgtgacc cgcatcagct tttcccgctg cggggggatc tttcccaccg
      661 gcacagtaag cgctgcgtcc gcaaagtagc cgctcagctc gacgccgaaa tccatcgaaa
      721 gaatgtctcc ctctcgcacg cggcgcgaag aagatgggat tccgtggacg acctcctgat
      781 tgatggaggt gcacaccgaa cccggatatc cacgatagcc tttaaatgcc gggcgcgcac
      841 gatgctcggc tgtgtaggtc tccgcaaact cgtccaattc cttggtggaa aggccgggcc
      901 ggaccatctc gcgcattttc tccagcgcgc cccacacgag caggcctgcc tgatgcatct
      961 tttcgagctc agccgacgat ttacagatga ccatcacgtc cttccgcgcg cctgaaaatc
     1021 tcaccgagcg cacggcccac atcttccaca tttgccgcgg cgttcacggt atcgagaagg
     1081 ccctggcggc ggtaataatc cgcgagaggc ttggtctggc gctcgtaggc cgccaaccgt
     1141 tctttcacca cttcggggcg atcgtcgggc cgctgcacca gcactccgcc atccgtgtcg
     1201 caaatgccgg gcgtcctcgg aggcgcatcg tacacattgt aaatctcgcc gccgacgctg
     1261 cacgtccaac ggcccgaaag gcgccgaaaa agctcgccgt cttcgacctt gaaatcgacg
     1321 acgatgggct tgccgaaacc cttgcgctca aggatgcggt cgagctgttc ggcttgctgc
     1381 aatgtccgcg gaaagccatc gaacacaaat cctttggcgg agtccggctg cgaaagccgc
     1441 tcttccacca tcctcatcac cagatcgtcg ggcacgagat cgccgcgctc catgatcggc
     1501 cggacgattt ggccgagcgg cgtgccgcgg ttaatggcgt cgcgaaacat gtcgccagtg
     1561 gaaagatgcg gcacgccata gcgttgcgca attcgcttcg actgagtgcc cttccccgct
     1621 ccgggtgggc ccaagaaaat caacgcccga ttcagaggca tcctcaccgc cgaaacactg
     1681 gccatgtttg tttaaccgcg ccggcttcgt acccggcctt tgcgcacgaa cccttcgtaa
     1741 ttgcgcatga cgagctgcgc ttccaactgc tgcaccgtat ccatcgccac gccgacaaca
     1801 atcaggagcg acgtgcctcc aaaataaaat tgcacgccga gcccatcgag gatgaagcgc
     1861 cacgtatggg tatcaaacca attgccgccc agccaggtgg gcaagtgctg caggtggatg
     1921 cccgaaatca tccattccgg cagaaggcaa acgacggcga gataaacgcc acctacgaac
     1981 gtcagccggg taagtattcg attgatatgt tcggacgtgt ttcgtcccgg acggattccc
     2041 ggcacgaacc cgccatactt ccgcatgtta tccgccagct cggtgggatt gaaaatgatg
     2101 cccacataga agaaggcgaa gaagacgatc agcacgacat acaacagggt ataaagcggc
     2161 tcaccccagc caatcatttt gccgagcgcc tgtatggccg gcgagcggct ctggaaaatc
     2221 agcgccaaag tctgcgggaa tgtcagtaag gaactggcaa agatcggagg catcacgcca
     2281 ccggaattca cgcgaagcgg cagatacgtc atctggccgc ccatgacctt gcgccccatc
     2341 acacgcttgg catactgcac gggaacgcgc ttctggccgc cctccacaaa gacaatgaaa
     2401 gcgacgaccg caatcatgaa aatgagcaag gcaatgatgg tgatgggggt aaaggagccc
     2461 cattgctcgg tccgaacctt ttgccacaag tcctggatag cgcgcggcag gccgactacg
     2521 atgcctgcaa atatgatgag cgacatgccg tttccgattc cgcgatcgct gatctgctcg
     2581 cccagccaca tgatgaatgc cgaacccgtg gtaagagtca gtatagtcat cagcatgaac
     2641 ctgtacccag ggtgataaac gagggactgt ccggcaaggc cctgggccgt gagtgtgcgg
     2701 gcgatcgcga aagactgaaa cacgctgaga atgatggtga gatagcgcgt gtattgcgtg
     2761 atcttgcggc ggcctagttc gccttccttt tgcaggcgct ccagataagg ccacaccacg
     2821 acgagcaact gcaaaatgat cgaggcggtg atgtacggca tgatgccgag cgcgaagatc
     2881 gtgagcctgc ggaaattacc gccgctaaag agatcgataa tgcccagcac cgatcctttg
     2941 ttctgctcga aaagctgctg aaggaggtcg gtattgatgc ccggcgtggg aatgaacgcg
     3001 ccgagccgat acacacccag cacccccagc gtaaacaaca cacgtttacg gaggtccggc
     3061 gtgctaaaga tattgcgaaa ggcctcgacc agcttcctca tgacaagact tcggtcttcc
     3121 ctccggcgcc cgcgattttt tcctgcgcgg atttgctgaa cgcgtgggcc cgcaccgtca
     3181 gcgcaacttt caattcgcca ttgcccaaaa ctttgatacc ggagcggctg gcgcgcacga
     3241 tgccgcgctc agtgagcgat tccggcgtga ctgtgtcacc tgccgcaaac gcgttgagat
     3301 tttcgacgtt cacgacggcg aaagttttcg aaaaaggcgc tgaaaaaccg cgcttcggca
     3361 agcggcgatg aagaggcatc tggccacctt caaaacccgg gcggcgagaa tatccgcgac
     3421 gggactgctg tcccttattg ccggcgccag acgttttgcc gagcttcgag cccatgccac
     3481 gccccacgcg caccttccga tggcgggaac cgcggggcgg cttcaaattc gagagtgtcg
     3541 gcacgttagc catattcgct attcctcaat ttcaaccaga tgatgaacct tcgctatcat
     3601 cccgcgagtt tcgggtgtgt cctggcggac tacaacctgc tgcatgcggc ggaatccaag
     3661 accgcgaatg gtctggcgca tgccgcgcgg gcagccaata aagctgcgca cccatttgat
     3721 tttcagcgtt cccgattctt tcttctttgt ggccatgatt tgggacctta attttattgg
     3781 ccggttgcga ccggcgcttc ctcagcgctc ttgccacgag tttcagcgac tctcgccgca
     3841 tcttttaaat ggaggagcgc gtcaaaagta gccttgataa cattgtgcgg attcgacgtg
     3901 ccgagcgact tggtcagcac gttttgaatt cccgcaacct ccatcaccgc gcgaacagga
     3961 ccgcccgcaa tcacgccggt tccttccggc gcgggtttca aaagaacctg cccggagccg
     4021 tatgacccaa gcacttgatg cggaatcgtg gagcccttca ggttaagctt caccatattt
     4081 ttcttggcct gctcgatagc cttgcgaatg gccatgggaa cttcgcgggc cttacccatg
     4141 ccgaagccag cgtgtccgcc ctgatcgccg accacgacga gcgccgaaaa gctgaggttc
     4201 ttgccgccct tcacgacttt cgtgacgcgg ttgatcgaaa ccacctggtc cttcaggttg
     4261 gcctgattgg tctgaacggt gcggcggatg gaaagcgtat ctttgagctc ttgtttcggc
     4321 atagttttca gaacttcagg cccgcctcac gggccgcttc ggcgagcgcc tggacgcggc
     4381 cgtgatattg ataaccgccg cgatcgaata cgacctgttc aatgcccttc gcgcgggcgc
     4441 gatcggcgac gatttttccc acgactttgg aagcggcgat gttgccaccg ctcttgatcg
     4501 cggcgcgcac ttccttatcg agcgaactgg ccgagaccag ggtgcgcccg gcgccatcgt
     4561 ccaccacctg cgcgcgaata tggcggctcg aacggtgaac gcaaaggcgg ggccgctcca
     4621 tggtgccctc gaggcgtttg cgcgtgcgca cgtgaattcg ccggcgatgc gcctcgcgtg
     4681 ataattttcg gacaggtgcc gccaatttcg ctctcctttt cgagccgccc tacgcagcgg
     4741 tcttcgctcc ggccttgcca gccttcttct tgaggcgctc tcccgtgatg cgaacgccct
     4801 tctgtttgta cgggtcagga ggacgcaagc gacgcaagtt cgccgccacc tggcctactt
     4861 gattcttatc cgctcccgtt acagtgaggt gggtttgctt ttccactgcg atttgaattc
     4921 cttccggaat cggaaactcg atgggatgtg aatatcccag cgccagcacc acgttcttgc
     4981 cttttacttc ggcgcggtag ccgacaccga cgatatccag atctttcttg aagccttgcg
     5041 tgacgcccat gaccgcgttg gcgacaagcg cgcgcgccaa accgtgaagg gcgcgaaatt
     5101 cttccgcctc gcgcttggcg acgagcgtgc catccttctg ctcgaactga attccgcgag
     5161 gcaagccgac cgtcatcttg cctttggggc cttgcacttc gaccgcgtca gctttgatct
     5221 gaatcttgac gccggacgga acggcaatca gtttatttcc gattcgcgac atagttctta
     5281 tgaacctcgg ttaccaaact tcgcacaaca gctcaccgcc aacgccttcc ttgcgagcag
     5341 cttgcccggt catcagcccg cgaggagtgg agacaatggc gatccccatg ccgccgacaa
     5401 cgggacgaat tccaaatttc ccgacgtagc gccgggagcc aggacgtgaa acgcgcttca
     5461 ggtcggtgat cacgctgcgg cgctccggcg tgtagcgcaa gaaaacgcgc agtgttttct
     5521 tgcccttttc ctcgacgacc ttgaaattga cgacgtaacc ttcgtccttg agaatgcggg
     5581 cgatctcgat tttcattttc gatgacggca tatccacgcg cgagtgcctg gcccgcgagc
     5641 catttcgaat tcgcgtcaaa aaatcagcaa tgggatcagt tgtcattctt cggtttcctt
     5701 accagctggc tttcacgacg ccgggaatct ctcccttgag cgcgaggccg cgaaaacaga
     5761 tgcggcacat ctgaaacttc cgcagcgtgc cgcgcgcccg tccgcaaatc cggcaacggt
     5821 tgcgctggcg aattttgtac ttcagcttgc gctgagtctt ggcaatctga ctggttcgag
     5881 ccattctgag ccgctctcct tacgcctgcc cttgagcgcg gaatggcacg ccgagcaact
     5941 taagcagctc gcgcccttct tcatcgctcc gggcagttgt ggtgatggtg atattcatgc
     6001 ctttgatttt atccacgcgg gtgtaatcga tttccggaaa gaccaactgg tctttcaaac
     6061 ccaaagtgta atttccgcga ccatcgaacg aattcggggc aagtcccttg aaatcgcgga
     6121 cacggggaaa aacgatcgcg gagagccggt cgaggaattc gtacatccgc tctcctcgca
     6181 aggtgaccat gcaaccgatg ggcatgccct tgcgaatttt gaaattcgcg atggacttct
     6241 tcgcgcgggt aatcaccggc ttctggccgg caatctggcc cagttcgacc gcggcggtat
     6301 cgagcaactt cacattttga ctggcctcgc caagtcctat attgagcgtg accttggaga
     6361 cgcgcggaac ggccattgtg ttcttatagc cgaaccgctt ggtgagcgct ggaacagcgt
     6421 ctttgcggta cttttcgtgt aagcggctgc tcattcttct ctgcctcgac taattctcaa
     6481 aagtggtgtt gcaccggcgg catacgcgca ctttcgtgcc gtcgggcagc gcggtatggc
     6541 ccgcgcgggt atgcttgccg catcccgggc aaaccagcat cacgttggaa atgtggatcg
     6601 ggccttcttt atcgagaatg ccgccccgaa tattcttcga cggattggga cgagtatgcc
     6661 tcttgataat gttggcgtgc tcgacggtca cgcgagactt ttccagattc accgaaagta
     6721 ctttgcccgt cattcccttt gagcggcccg ccatcacttt cacggtgtcg ccgtggcgta
     6781 tacccaaggg agccggcttt tcagctattt ttctcatggc ctaccacact tccggagcga
     6841 gcgagacgat cttggtaaac tttttgtcgc gcaattcccg tgcgaccggc ccgaagactc
     6901 gggttccgat cggctcgttc gcgtcattga ttagcactgc tgcgttgtcg tcaaaccgaa
     6961 tgtaactacc atccttgcgc cgctgttctt ttctggtgcg cacaaccacg gcccggacta
     7021 ctttgccctt tttgacctgg ccgtccggcg tagcttcctt gacggcggcg gtgatcacat
     7081 cgcccaagcc agccgtaagg cccgcgtccc cgccaattgg gagaatgcag gttatctgcc
     7141 gggcgccgga attatcggcc accgtcagcc atgtccgcat ctgaaccatc gtcgcgctcc
     7201 cttgcccttc gttgttcgca ggcgcctacg cggcgcgagt tgaacgctgt atgacttctt
     7261 tcagccgcca gcgcttcagc ttcgaaagcg gacgcgtagc ggcaatccga acaatgtcgc
     7321 cgacgcgggc ttcgcccgtt tcgtcatggg cgtaatactt tttccctgat cgcacaacac
     7381 gctgataaag ggggtgctgc gttgcgcgcg tgaccttcac gacgatcgtt ttctgcatct
     7441 tcgcgctggt gaccacgccc accagctcct gccgaattcc gcgctcgcgg acgccttggc
     7501 cggactttgg cgtgtcgggg gccattgttt cgtttgccat tacgccttcc tcaattcgac
     7561 ttcgcgcaac aaggttttca cgcgcgcgaa atcttttttc gcttcccgga gttttttcaa
     7621 gccctcggcc tggccagccg cgatttgaaa gcgcagccgg aaaatttgct ccgccagttc
     7681 gcgttcctgg accgtgagct cgtcggaatt catttcgcga tatttctcaa cgctgcgcgc
     7741 cactagagtt cctccgagcg ggtgacgaac ttcgtcgcga tgggcaattt gtgagcggca
     7801 agttccatgg cttccgtggc ggaagccaaa tccacgcccg ccatttcaaa catgatccgc
     7861 cccggcttca cgggagcaac ccaatgttcc ggtggaccct tacctttgcc catgcgcgtt
     7921 tcggccggct tcttggtgaa aggcttgtct ggaaaaatgc gtatccacag tttcccaccg
     7981 cgttttatga acctggtaat cgccacgcgc gccgcttcga tttgccggtc ggtgatccac
     8041 gcgggctcga gcgccttcaa tccatactcg ccgaaagcga gcgagcctcc gcgccacgcc
     8101 tttcccgtgc ggcggccgcg ttgctgcttg cggaacttaa ctttttttgg catcaacata
     8161 aattttcgtc tgctctcttt tagatcagcg ctccggcggc ggccggcttg gagcgtagcg
     8221 gaactttttc gccctgatac acccagcact tcacgccaat caggccgtat gtggtgtgcg
     8281 cttcagcggt gccgaaatcg atgtctgccc gaagggtttg cagagggagc cgcccttgca
     8341 gataccattc cttccgggcg atttcggcgc cgttcaggcg tcctgccacg cgcactttga
     8401 ttcctttgca accgaagcgc agggccgaat ccaccgcttt gcgcatcgcg cggcgaaacg
     8461 ccacccgctt ttcgagctgc agcgcaatgg actccgctac gagcgtggca tcgagctctg
     8521 gccgatgcac ttcctgaatg tcgatgtgaa cttcgcggtt ggttcgcttc tggacttcgg
     8581 ccttcagctt gtcgatttca gaacctttgc ggccaatgat gattccgggg cgagccgtgt
     8641 aaatccggac taccagtttg ttggccgctc gttcaatatc gattccgttg atgcccgctg
     8701 aacgcagcct gtctttcagc tgagtgcgca gcttgacgtc ttcatgcaac aggtcggcat
     8761 agtccttttt tgcgaaccag cgagacctcc aacctttgtt gtagcccaac cgaaatccgt
     8821 acggatgtgt tttctgcccc aaaacttgcc tccgctctta ctttttcttc tgcgcttcct
     8881 tcgcgcgttt ctcgatggcc ttccgagcct ttcggaccgc accgcgcacg cccttggacg
     8941 cttcggcctc ggcaactgct gctgccgcgc ggtccgccgc ggcgcggtga tgttcggcta
     9001 cctgaactac gatatggctg gtgcgctttt ggtagcggaa acctcgcccc atcggcgcag
     9061 gacgcatgcg cttccaacgc ggaccttcgt tgacacagca accggtgacg tatagcgcgt
     9121 ccacgtcgag cgactctccg gcgtcgtcgg ccttgcgctc ggcgttggcg atggcgctgc
     9181 gcaacgtttt ttcgatttcc ttggccaccc gtttttttgt aaaacgcagc gtttgcaagg
     9241 cgtcttccgc gcgacgccca cggatcaaat cgatcaccag ccgagccttc tgcggcgaga
     9301 cgcgaacgaa ccgcgtgttt gccatgcctt caatctgact ttgcgccaac gttgccatca
     9361 tctattatcc cttcacctac gacttgcccg ccggaggcgc gcctccagcc ggagcagcgg
     9421 caccgccggg accggctgcc gcagcggcgg ctctttcgag cgcaaccttt acgccgtgtc
     9481 ccttgaacgt acgcgttggg gcaaactcac ccagcttgtg ccccaccatc tgctcggtga
     9541 tgtacacggg aatgaatttc ttcccattgt gcaccgcgat cgtatgaccc accatttccg
     9601 gcaggatcgt cgagcggcgc gaccacgtct tcaccacttt cttttcgttg cgagcgttca
     9661 gcgctgccac cttttcttgc aggtgggtat cgatgaacgg acccttcttt agcgaacgcg
     9721 gcatcggttt cctactcctc gaccctgcgg agctttaccc actgcgcgcg gcgcatcttg
     9781 cggaatccgc gcacgggacc cggcacgcgg tgcgaaagcg ggcagaaggg gcaaacaaac
     9841 cgcgtccctt gcgcattggg tatcttatcc acaattacgg cccatcccgc gcggcaaagc
     9901 cggaacagaa cctgcggcgc cggaccgagc ttcgacactt tgcctacggg cttgattgcg
     9961 ggcttagcaa ccggcttcac agcgcgccgc gaaactgctt ttcgcgctcg caccgctcgc
    10021 cgtggtgcac gccgagacgt tcgtgtagct gtcatagagc tgctctcaat tccccgccgc
    10081 gccggatgca tttttactgc ttcaccaaaa agtgtttcct cttccctacg gcttgcgccg
    10141 ttcgacaatc attcgatcgc tcttacgctt ctttcgcgtc ttcgcgccca gtgtcggaaa
    10201 tccccacgga gtttgcgggt ggttgccttt cacgcgacct tcaccgccgc cgtggggatg
    10261 atccacaggg ttcatcgcca ctccgcgcac cgtggggcgc ttgccatgcc accgggtgcg
    10321 gcccgccttc ccgtacgtga cgttctcgtg atccaggttg cccaactggc cgacggtagc
    10381 catgcaatcc acgctgaatt ttcttacttc tcccgaaggc agcttgacca gcgcgaaatc
    10441 gccttctttg ctcagaagct gcgccgctcc gcccgcagcg cgcacaacct gcgcgccttt
    10501 gccaggaaac agctcgaggt tgtgaaccat cgttccaggc gggatgtgcc gaatggtcaa
    10561 tgcatttccc ggaagaatgt ccgcaccctc gccgttctgc accgtcatcc caacttcaag
    10621 cccaacgggc tggagaatgt aacgcttctc gccatcggcg tagtgcagca gcgcaatccg
    10681 cgccgagcga ttcggatcgt attcgatggc cgccaccttc gccggaattc ctatcttttc
    10741 gcgacggaaa tcgatggaac gaaatctgcg agcgtggcct ccgccgcggt gccaggacgt
    10801 cacttcgcca tgcgcgttgc gtccgcccgt gcgcttcttc gcttcagtca
//
LOCUS       DATNDT010000457         9202 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3288929, whole genome shotgun sequence.
ACCESSION   DATNDT010000457 DATNDT010000000
VERSION     DATNDT010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9202)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9202)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9202
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3288929"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..255)
                     /locus_tag="VN788_15005"
     CDS             complement(<1..255)
                     /locus_tag="VN788_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HXU21889.1"
                     /translation="MRILLFSGKGGVGKTSLAAATGVKLAELGYRTLVMSIDSAHSLA
                     DSFDLDIDLFHVQTSDPFSIQDGLSILELNIQKEVKRHWQE"
     gene            complement(336..1493)
                     /locus_tag="VN788_15010"
     CDS             complement(336..1493)
                     /locus_tag="VN788_15010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21890.1"
                     /translation="METREKSTMKEVVPSPSRALVVRSAAIVARGLRDLARDSNWLLK
                     KVFSGRSSHLAISQHGELCALSPMVRHGTERLTLYDIELSVPTLALAIPGEPSVSVPG
                     LPAVFAWSPAGRYLTAAWGGWLPELHVFDIDGKAYLGAFGDFAHFPTSLVWSSAGKYF
                     ACASGGGEGARLRLWEAAPQTSSAMPFAAEPSIELGAPPALDVTAEGEFAEHSDDEAD
                     DHGVFSGYGRTTFSPDENVLATVLENDGDWADDSIALLEVPSLRMQKISQAQGRVTDL
                     SWTFDSHQLIYCAAGQAYRIVPESGAAEPLPFGAELCACHPHLPLGLFFSAWLKNSAK
                     GRIFVADLNCLRVFDEYPAEGVVDLRWSADGAKAYAVTAEGLAYIYEPPLL"
     gene            complement(1528..2343)
                     /locus_tag="VN788_15015"
     CDS             complement(1528..2343)
                     /locus_tag="VN788_15015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HXU21891.1"
                     /translation="MEIKAAEARPGTQAAQAFVQPPVKPEDQELARKREEQAALEAEL
                     AERELRSANLRAELGAFERQYLHFVGSRYAELDELKAQLAERLAQEQPGNQRAQKAAR
                     DARARADETKSVAGEKSIKEPRAFESSPEMKRLYREVAKRVHPDLTSNREDRAKRQQL
                     MAEANEAYERGDEAKLTKIFTEYECSPEAVEGEGAGAELIRVIRRVSQTRGRLAEIEA
                     EAQEMLRSDLYRLKSRVDEAGAAGRDALNEMIAKVEEQIALAKRRLEDRESPD"
     gene            2604..3116
                     /locus_tag="VN788_15020"
     CDS             2604..3116
                     /locus_tag="VN788_15020"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU21892.1"
                     /translation="MQETADQYRKRMLGHLEGRDPLKLQAGAPAKLAKLLKGVSPAKA
                     RKRPAPGKWSIAEIVAHIADTELVGGYRIRAILGQPGTQIIGFDQDAWVTALHYDKRD
                     LRKSFEQYRGLREANVALLKSLTPEQWKHHGMHSERGEESVETIVLMFAGHDINHFAQ
                     IKRILSAKRN"
     gene            complement(3124..5814)
                     /gene="glgP"
                     /locus_tag="VN788_15025"
     CDS             complement(3124..5814)
                     /gene="glgP"
                     /locus_tag="VN788_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HXU21893.1"
                     /translation="MNATTEGIQNVTGNGQSRDATYVLTLEEIGDLAREGGKPAETLM
                     NVVKLIARRFQTEVCSVYLLEPDRASLLLAATVGLRPQSIGSLHLGLNEGLAGLVAEQ
                     VRPVAVEHAQAHPRFKYFKEAGEDAYQSFLGVPLIDRGVLQGVLIVQTIEPRVFSDDD
                     IRMLLGAAAQVAPVVTQARALGNFIAPAQERLWALARNLWWSWDHDSTSLFRDLDPVR
                     WRALNHNPVALLKELPAQEIERRVAELVLHSRINYAYRRQREYLEADRTWGAMHAGVL
                     RPRPVGYFSAEFGIHESVPEYSGGLGVLAGDHIKSASDLGIPLIGVGLFYGQGYFRQW
                     LDSSGWQQEEYLPTDVNHLPMELAIGANGEAVTVQIDTRSGSIRAKVWRMTVGRCDLL
                     LLDSNVEGNAPEDLELTSRLYGGDGRIRIRQELLLGIGGFRALRAMGVAPGVLHLNEG
                     HSGFAVLEAVRDAMQEEGLTFAQAVSRVSRGVVFTTHTPVPAGHDRFDANLIEEHLGP
                     LREALGLSHDSLLALGREKPENNAEDFCMTVLGLKLSRRANAVSALHGEVSRAMWTGL
                     YAGKSEDAVPIGHITNGVHVPTWLAPQMHRLYVRHLGTGWHEHSSEARLWQGIESVDD
                     GELWETHLNLKGQLLEFVRRRAVNQAARRGESPEMLKALGSVLSQDALTIGFARRFVT
                     YKRANLILADIERLTSIVNDPSRPVQFVFAGKAHPRDEPGKRVLQQIAELRRDPRFAG
                     KFVFIEDYDINVGRHFVRGVDVWLNNPRRPLEASGTSGQKVVLNGGLNLSVLDGWWAE
                     AYDGLNGFAIGTGRTHSNMDVQDSRDGEALYRTLCNEVIPLYYQRDRDGLPRGWIKRM
                     KRTIRTLGWRFNADRMVMDYTLKCYVPAAGGTSSDMRPSS"
     gene            6117..6530
                     /gene="msrB"
                     /locus_tag="VN788_15030"
     CDS             6117..6530
                     /gene="msrB"
                     /locus_tag="VN788_15030"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011396194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HXU21894.1"
                     /translation="MAEKVRKSDAEWRSKLTDEQFHVTRKKGTEPPFSGEYEDTETEG
                     TYVCVCCGQPLFASDAKYHSGSGWPSYYQPIAADNVEMDRDTSHGMSRTEVMCSRCDA
                     HLGHVFEDGPPPTGLRFCINSAALKLLEKDSDAKK"
     gene            complement(6570..>9202)
                     /locus_tag="VN788_15035"
     CDS             complement(6570..>9202)
                     /locus_tag="VN788_15035"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HXU21895.1"
                     /translation="TIEIVPPAAGNSVDVGSTQALTFTALVGGDSKNAGVSWTLTGSG
                     CSGTGCGTLSNSTPQAVTYTAPASLPSTTALSVTLTATSVTEKSVTQTATINVEPAPT
                     FLTTECNPTGVLPCVLPNGENGVGYSTTISFTGGVAPYRFNMPALPACLQLVTSTSGT
                     TAAISGRPCGSGSTTFTVTLMDSGGAAAVSQQFTITISPPPGLSITTTSLPPSTVDAT
                     YASAVATQGGVAPLAWSVISGALPPGVVLNPANGKITGIPTTAGTFSFTVQVKDSALP
                     TSQTATQAFSVTIATPAPLSITTSSLAAGVTATGYSGTLQATGGVLPYTWSLTNGQLP
                     AGLKINSSGTITGTPVVAGTSTFTVQVADSEVDPATGNPNPMIATKQFTITVTAGTNN
                     DKLFFNGYSFLFTGFDKDGSMAIAGFLTTDGQGNITSGGEDITRVSKVVTGATLTGTY
                     SIDSNGDGHGTMEIVATLGQNTLVTDYRLVLDSSGNVKFFEDKPATANGDTLQTHGEG
                     VMKPIVGSTFSAVNLSGNYAFEFAGQDLSQKRAAFGGVLNADGNATFSSGLSDFNDAG
                     TFSSGSVSGSFSFIAGNRGTASLTFPLPSGQATLNFVYYFVSPSDLYLVEVDSSQGNG
                     TPTEFRLAGEMILQQPTTAFTSSILQGVSVASGSGVSGANANADVFAGLLTATVCNGS
                     TAVTLAYDENNGGAIAAPSFSGTCTVNPKGRASFSGLGATAADERVAVAYLTGPGQGF
                     ILGSDSAVTTGLLEQQSGGPTFANSSVLDGYTLSAPFTAETQVKNIVGEVTADGAGNI
                     SGTVDEIDPPATGTPNLAQALSGALSIQGSGRGTLTPAGPVPSGLPASAIFYLVSPAK
                     IRVVSNDPTDQHPELIFLDH"
BASE COUNT         1935 a         2904 c         2570 g         1793 t
ORIGIN      
        1 ttcctgccag tggcgcttca cttccttttg gatattcagc tcaagaatcg agagcccgtc
       61 ctgaatcgaa aagggatcgg aggtctggac gtgaaacagg tcgatatcca ggtcgaacga
      121 atccgccagg ctgtgggcgg aatcgatgct catcaccaaa gtgcggtagc cgagttcggc
      181 cagtttgacg cccgttgccg cagccaggct ggttttgcct acgccgccct tgccgctgaa
      241 tagcaaaatc ctcatgcgcc tattttatac gcaaattcac gccagcgaat cgaagtaaaa
      301 gcgcgcgacg aggatgcgct cgtcgtgggt acaggctaaa gaagaggcgg ttcgtaaatg
      361 taggcgaggc cctcagcagt gacggcgtat gccttcgcgc cgtccgcgct ccagcgcaga
      421 tcaacgacgc cctccgcggg atattcatcg aaaacacgga ggcagttcaa atcggcaacg
      481 aatatgcggc ctttcgcgga gttcttaagc cacgcgctga aaaataatcc cagcggcaag
      541 tgcggatggc atgcgcaaag ctctgcgcca aagggcagcg gctcggccgc gcccgattcg
      601 ggcacaatcc ggtaagcctg tccggctgcg caataaatca gctgatggct gtcgaatgtc
      661 cagctaagat ctgtgacgcg tccctgtgcc tgagaaattt tctgcatgcg caacgacgga
      721 acttccagca gcgcgatcga atcgtctgcc caatcgccgt cattctcaag gacagtggcg
      781 agcacatttt cgtccggact gaaagttgtg cggccgtagc cggaaaatac gccgtgatcg
      841 tccgcctcgt cgtccgaatg ctcggcgaat tccccctcgg cggtcacatc gagcgcaggc
      901 ggtgcgccaa gctcaatgct aggctcagca gcgaacggca tcgcggagga cgtctgtggc
      961 gccgcttccc acaatcgcag ccgggccccc tcccctccgc ctgaagcgca ggcaaaatat
     1021 tttccggcgc tcgaccaaac caggctcgtt gggaaatgcg cgaaatcgcc aaaggcgcca
     1081 agatacgctt tcccgtcgat atcgaacacg tgtaactccg gcagccatcc gccccaggct
     1141 gccgtcaggt atcggccagc gggcgaccac gcaaacacgg caggcaaacc cggcaccgaa
     1201 actgacggct ccccgggaat cgcgagcgcc agcgtgggca cgctcagctc aatatcataa
     1261 agtgtgaggc gttccgttcc atggcgcacc atcggcgaaa gcgcgcacaa ctccccatgt
     1321 tgtgaaatcg ccagatgcga cgagcggcca ctgaaaactt ttttcagcag ccagttggaa
     1381 tcgcgcgcca ggtcgcgcag cccgcgcgcc acaatcgcag ccgaccgcac caccagcgct
     1441 cgtgatggcg acggaacaac ctctttcatc gtcgattttt cgcgcgtctc cataaagtag
     1501 ctttgctgct ctctcgatct cgcttctcta gtcgggcgat tcgcgatctt ccaaacgccg
     1561 cttcgccaga gcaatctgct cctccacctt cgcaatcatc tcgttcagag cgtcgcggcc
     1621 cgccgctccg gcctcatcca cccgcgattt aaggcggtac aaatccgagc gcagcatttc
     1681 ctgcgcctcc gcctcgattt cagcaagcct gccgcgggtc tggctcacgc gccggatcac
     1741 gcgaatcagt tcggcgcccg cgccttcgcc ctcgacagct tccgggctgc attcatattc
     1801 ggtgaaaatt ttcgtcagct tggcctcgtc accgcgttcg taggcttcgt tcgcttcggc
     1861 catcaattgc tggcgtttcg cgcggtcctc gcgattggag gtaaggtcgg gatgcacgcg
     1921 cttggcaact tcacggtaga gccgtttcat ttcgggcgaa gattcgaaag ctcgcggctc
     1981 cttgatggat ttttcgcccg ccacggactt cgtttcatcg gcgcgagcgc gagcgtcgcg
     2041 agcggctttc tgtgcgcgtt ggttcccggg ctgctcctgc gcgagccgct ccgcaagttg
     2101 agcctttaat tcgtcaagct cggcataacg cgaccccacg aaatgcaagt attgccgctc
     2161 gaaagcgccg agctccgcgc gcaagttggc cgagcgcagc tctcgctcgg caagttctgc
     2221 ttcaagagca gcttgctcct cgcgctttcg cgccagttcc tggtcttcgg gcttgaccgg
     2281 cggctgaacg aacgcctgtg cggcctgcgt cccaggacgc gcctccgccg ctttgatttc
     2341 cattgtgttc gcctctgaga gctcgaatca aggcaagcgg ccggctacat cccaagcggc
     2401 agcatgccct gaatcactta aattgtacga tgcgggcgta accgcccggc gagcttggga
     2461 agctcagttt ccaagatgga aaacggacac tggccaattt gtctctcccc cggataagcc
     2521 ttgtgctggc cgtggttcca gaaaagtacc tggtattcta aaatcagagc caatcagccg
     2581 tcactcggcg caggaggaat cgcatgcagg aaactgcaga tcaatatcgc aaacgaatgt
     2641 tgggccacct ggaagggcgc gatccattga agctgcaagc gggggctccc gcgaagcttg
     2701 ccaagttgct taaaggcgtg agtcctgcga aggctcgcaa aaggccggcg ccggggaaat
     2761 ggtcgatcgc ggagatcgtg gcgcacattg cggataccga actcgtcgga ggctaccgaa
     2821 tccgcgcaat tcttggccag ccgggaactc agatcattgg cttcgaccag gacgcctggg
     2881 tcacggcgtt gcactacgat aagcgcgacc tccggaaatc attcgagcag tatcgcggat
     2941 tgcgcgaagc caatgtcgcg cttctgaaat ctcttacgcc ggaacaatgg aagcaccacg
     3001 gcatgcactc cgagcgcggc gaagaaagcg tggagacgat tgtcctgatg ttcgccggcc
     3061 acgacattaa ccatttcgcg cagatcaaac gcatcctgtc ggcgaagcga aactagcaaa
     3121 cgattaactg ctggggcgca tatcgctgga ggtacctccg gctgccggga cgtagcattt
     3181 cagcgtgtaa tccataacca ttcggtcggc gttgaagcgc cagccgagcg ttcggatggt
     3241 ccgcttcata cgcttgatcc agccgcgggg aaggccatcg cgatcccgct ggtaataaag
     3301 cgggatcact tcgttgcaaa gagtgcgata cagagcttcg ccgtcgcggg aatcttgaac
     3361 gtccatattt gaatgcgtcc tgccagttcc aatcgcgaag ccgttcagcc cgtcgtacgc
     3421 ctctgcccac cagccgtcca gcacggaaag attcaagcct ccgttgagaa ccaccttttg
     3481 gccacttgtg cccgaagctt ccagcgggcg ccggggattg ttgagccaca catccacacc
     3541 acggacgaaa tggcgtccca cgttgatgtc gtaatcctca atgaaaacga atttgcccgc
     3601 gaagcgcgga tcgcgcctca gttcggcgat ctgctgcagc actcgcttgc cgggctcgtc
     3661 gcgcggatgc gctttgcccg cgaacacgaa ctggacggga cgcgacggat cgttgacgat
     3721 cgaagtaagc ctctcgatat ctgccaggat caaattcgct cttttgtaag tgacgaaacg
     3781 ccgcgcgaat ccgatggtga gtgcgtcctg gctcagcaca cttcccagcg ctttgagcat
     3841 ctctggagac tcgcctcgac gcgcggcctg gtttacggcg cggcggcgga cgaactccag
     3901 aagctgtccc ttcaaattga ggtgcgtttc ccaaagctcg ccgtcgtcca cactctctat
     3961 gccttgccaa agacgagctt cgctgctgtg ctcgtgccag ccggttccta gatggcggac
     4021 gtagaggcga tgcatctgcg gcgcgagcca agtgggaacg tggacgccgt tggtaatatg
     4081 cccaatggga acggcatcct cggatttccc tgcgtagagg cccgtccaca tggcgcgcga
     4141 cacttcccca tgcagagcag aaacggcatt ggcacggcgg gaaagcttga ggcccagcac
     4201 cgtcatgcag aaatcttccg cattgttttc aggcttctcg cgtcccagtg ccaaaaggct
     4261 gtcgtgggag aggccgagag cttcgcgaag aggacccaag tgctcttcga tcagatttgc
     4321 atcaaatcgg tcgtggccgg cgggtacggg cgtgtgcgtg gtgaagacga ctcctcggga
     4381 aacacgcgag actgcctgag cgaaggtcaa gccttcttcc tgcatcgcat cgcgaaccgc
     4441 ttcaaggacc gcaaacccgc tatgtccctc attgagatgc aaaacgccgg gggcgacccc
     4501 catggctctg agggcgcgga aacctccgat gcccagcaag agttcctgac gaattcggat
     4561 gcgtccatca ccaccgtaca ggcgcgaggt aagctccagg tcctcggggg catttccttc
     4621 cacattcgaa tccagcagca gcagatcgca gcgccccacc gtcatccgcc agaccttcgc
     4681 gcgaatcgac ccactgcgag tgtcgatttg tacggttaca gcctcaccgt tggcgccgat
     4741 cgcgagttcc atcgggagat gattcacgtc ggtcggcaga tattcctcct gctgccagcc
     4801 gctcgaatcc agccactgcc tgaaatatcc ttgtccataa aaaagaccga cgccgatcag
     4861 cggaattcca aggtcggatg cgcttttgat atggtctccc gcgaggacac ccaggccgcc
     4921 cgaatattcc ggaacggatt cgtgaattcc aaattccgcg gaaaaatacc ccaccggacg
     4981 tggccgcagc actccggcat gcatcgcgcc ccaagtgcgg tccgcttcca ggtattctcg
     5041 ctggcggcgg taggcgtagt tgatccggct atgcaacacc agctccgcca cgcgccgctc
     5101 gatctcctgc gccggcaatt ccttcagcaa agccaccgga ttgtgattga gcgcccgcca
     5161 gcggaccgga tcgagatcgc ggaacaagct ggtggaatcg tgatcccaac tccaccagag
     5221 attgcgggcc aacgcccaaa gcctctcctg ggcaggagcg atgaagttgc cgagagcccg
     5281 ggcctgcgtc accactggag caacttgcgc ggcggcgccc agcagcatgc gaatgtcatc
     5341 gtcactgaaa acgcgcggct cgatcgtttg aactattaga acaccttgca gcactcctcg
     5401 atcgatcagc ggaactccga ggaacgactg gtaggcatcc tcacctgcct ctttgaagta
     5461 cttgaagcgc ggatgagcct gggcgtgttc cacggcgacg ggccgcacct gctctgccac
     5521 cagcccggct aacccttcgt ttaggccgag gtgcaaactc ccgattgact ggggacggag
     5581 acccaccgtc gcggccagca ggaggctggc tcggtctggc tcgagaagat ataccgaaca
     5641 gacttcggtc tgaaacctac gcgcgatcaa cttcaccaca ttcatcagcg tttctgctgg
     5701 cttaccacct tcacgcgcga ggtcgcctat ctcctcgaga gtcaggacgt acgttgcatc
     5761 gcggctctgg ccgtttcccg tcacgttctg gattccttcc gtagttgcgt tcatcggagc
     5821 gccaagttgc gccatcttaa ctcacctcat ttatctcatg ggccgagggt cgctgctcgg
     5881 cctgtgtcgt gccccgatca cagacatgca accccccgcc gcaactgtcg taaccgagtg
     5941 acgaaactgt agtgctgcgc tcgccatgag tccaattaaa attgtctatt gatcgcagcg
     6001 cacaaaatat tagccgcacg ggcgccacat ttacacaggc gccggacgtg cgcacggcct
     6061 ccgcctgatg taagctggga acggatattt tactatttga cgagagaaaa agaatcatgg
     6121 ctgaaaaagt gcgaaagagc gatgcggagt ggcggtccaa actaaccgac gagcaattcc
     6181 acgtcacgcg caaaaagggc accgagccgc cgttctccgg cgaatacgaa gatacggaaa
     6241 cagagggcac gtacgtgtgc gtctgctgtg gccagccgct tttcgcctcc gatgccaaat
     6301 accattcagg ctcgggctgg ccaagctact accaaccgat cgccgccgat aatgtcgaaa
     6361 tggatcgcga taccagccac ggaatgagcc gcaccgaggt gatgtgcagc cgctgcgacg
     6421 cccacctcgg gcacgtcttc gaagatgggc ctcctcccac gggacttcgc ttctgcatca
     6481 actccgccgc cttgaagctg cttgagaaag attccgacgc aaagaaataa atcgcaagcc
     6541 gcgcagtcgc gctccaccag cgccaaaaat caatgatcga ggaaaatcaa ttccggatgt
     6601 tggtcggtag gatcgttcga gaccacgcgg atttttgccg gcgacaccaa gtagaaaatt
     6661 gccgacgccg gcaagccact ggggaccggc cccgcgggcg taagcgtgcc gcgcccgctg
     6721 ccctgaatgc tcaacgcgcc ggagagtgcc tgcgcaagat tcggcgtacc ggtagcaggc
     6781 ggatcaatct cgtccaccgt gccggaaata tttcccgccc catccgccgt cacttcaccc
     6841 acgatattct tcacctgagt ctccgcagta aaaggcgcgc tcagcgtgta gccatccagc
     6901 acagacgaat tcgcgaaagt gggcccgccc gactgctgtt cgagcaatcc cgtggtgacg
     6961 gccgagtcgc tcccgagaat gaatccttgc cccggccccg tgagataagc taccgcgacg
     7021 cgctcgtctg ccgcggtagc acccaagcca ctgaaactgg cgcgcccctt cggattcaca
     7081 gtgcaagtgc ccgaaaaaga cggtgccgca atcgctccgc cattgttttc atcgtaagcc
     7141 agcgtaacgg ccgtgcttcc gttacacact gtagcggtca gcagccccgc gaacacatcc
     7201 gcattcgcat tcgcgccgct cacgccgctt ccgctcgcca cactcactcc ctgcaagatc
     7261 gacgaagtaa acgccgtagt cggctgctgc aaaatcattt cgccagccaa ccggaactcc
     7321 gtgggcgtgc cgttcccttg cgagctatcg acttccacca gatacagatc ggaaggcgaa
     7381 acgaaataat agacaaaatt cagcgtagcc tgcccgcttg gcaacggaaa cgtcagcgaa
     7441 gccgtgcctc gattccccgc gatgaacgag aagctcccgg agaccgagcc agagctgaac
     7501 gtgcccgcgt cgttaaaatc gctcaagccg gaagaaaaag tcgcgttgcc atcggcgttc
     7561 agcacgcccc caaatgccgc ccgcttttgc gacaaatcct gccccgcaaa ttcaaaagca
     7621 tagttgccgc tcaaattgac agccgaaaaa gtcgatccaa cgatcggctt cataacgccc
     7681 tcgccgtgag tttgcagcgt gtccccgttg gcagtcgcgg gcttatcttc gaaaaatttc
     7741 acattcccac tcgaatcgag caccagccga tagtccgtga cgagagtgtt ttgccccagc
     7801 gtcgccacga tttccatcgt gccgtggcca tcgccattgg aatcgatgga atacgtgcct
     7861 gtaagcgtgg cgccggtcac cacctttgaa actcgcgtga tatcctcgcc gcccgaagta
     7921 atgtttccct gaccgtcggt cgtcagaaaa ccggcgatgg ccatcgatcc gtccttatcg
     7981 aatccagtga aaagaaacga atagccgttg aagaaaagtt tatcgttgtt cgtccccgcc
     8041 gtaaccgtga tggtgaattg cttcgtggca atcatcggat tcggattgcc cgtagccggg
     8101 tccacttccg agtccgccac ttgcacagta aacgtcgaag ttcccgccac caccggagtc
     8161 cccgtaatcg tccccgagct attaatcttc aaaccggccg gcagctgtcc attggtaagg
     8221 gaccacgtgt acggcagcac tcccccagta gcctgaagcg tcccgctata tccggtggct
     8281 gtaactccgg ccgccaagct cgatgtcgta atggaaagtg gcgcaggagt ggcaatcgtc
     8341 acgctaaacg cctgcgtagc cgtttggctg gtcggcagcg ccgaatcctt cacctgcacc
     8401 gtgaacgaaa acgtccccgc cgttgttgga attcctgtga tcttgccgtt ggccggattc
     8461 agcaccactc ccggtggcag agcgcccgaa atcacgctcc aggccagcgg cgccactcca
     8521 ccctgcgtcg ctaccgcgct ggcgtaagtc gcatcgaccg tgctgggcgg cagcgatgtc
     8581 gtcgtgatcg agagccccgg cggcggagat atcgtaatcg tgaattgctg gctcacggcc
     8641 gccgctcccc cgctatccat cagcgtcacc gtaaaagtcg tcgatcccga gccgcagggc
     8701 ctgcctgaaa ttgcggccgt tgtgcccgag gtggaagtca ccaattgcag gcacgccgga
     8761 agcgcgggca tgttaaaccg atagggtgcc acgccccccg tgaacgagat cgtcgtgctg
     8821 tatccgactc cgttttcgcc gtttggaagc acgcacggca gcactccagt cggattgcat
     8881 tctgtagtca ggaaagtggg cgccggctcc acgttgatcg tcgctgtctg cgtgaccgac
     8941 ttttcggtca ccgacgttgc ggtcagggtc acgctcaacg cggtggtcga tggcagcgac
     9001 gccggcgccg tgtacgtcac tgcctgaggc gtcgagttcg agagcgttcc gcagccggtg
     9061 ccggagcagc ccgatccagt gagcgtccaa ctaacacccg cgttcttcga atccccgcca
     9121 accagcgcgg tgaatgtcag cgcttgcgtc gagcctacgt ccaccgagtt ccccgccgcg
     9181 ggcggcacaa tctcgatagt ga
//
LOCUS       DATNDT010000458        16919 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1557055, whole genome shotgun sequence.
ACCESSION   DATNDT010000458 DATNDT010000000
VERSION     DATNDT010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16919
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1557055"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..420
                     /locus_tag="VN788_15040"
     CDS             <1..420
                     /locus_tag="VN788_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21896.1"
                     /translation="GRQYVLGNVYYGFSPSHFWLRVDPIPEAIAQMADFQMRITVWDS
                     RETRITLRIDNERLAPGSVLEQAGVCLLHPEAAMSAAYEKILELSLARELFDLRGRRD
                     LLVSVALWKGGLPVDVLPAEGVLQVPLGEENFAWPPA"
     gene            complement(553..1824)
                     /locus_tag="VN788_15045"
     CDS             complement(553..1824)
                     /locus_tag="VN788_15045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU21897.1"
                     /translation="MKYTKYSRYEPGAADDIDLQDLMDRLSDFFLQSGFHSQYGFHEL
                     DMERARQQHMDELREAILRALEDGDLIPPELLEKLRQNPDLSQNEELRNMIDQIIQRM
                     EEEGFITQQQSARVTPPPSETPGGQIGKDQPQVEARFEITDKALDFLGFKTLKDLLAS
                     LGKSSFGRHDTRDLATGIESGGSSKAYEFGDTLNMDISGTLFNAVRRNGAGIPIELDY
                     PDLMVHQCEYQSSCATVLMLDCSHSMILYGEDRFTPAKRVALALSHLIRTQYPGDSLH
                     CVLFHDSAEEIPLGKLARVQVGPYYTNTREGLRMAQRILARQKKDMRQIVMITDGKPS
                     ALTLDDGRIYKNAFGLDPLVVTQTMEEVAKCKRAGILINTFMLASDYSLVHFVQKITE
                     LCRGKAYFTTPYTLGQYLLMDYVGRKTRNIH"
     gene            complement(1851..3335)
                     /locus_tag="VN788_15050"
     CDS             complement(1851..3335)
                     /locus_tag="VN788_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HXU21898.1"
                     /translation="MSQPKNLGELKRSSWSEEKLAGRTIRQELRENLLQKMEKDEPLF
                     PGVHGYDDTVIPQITNAVLSRHHFILLGLRGQAKSRILRGLAGFLDPQIPVIAGCEIN
                     DNPYRPLCRACRERIAAEGDGTPIAWLSRELRYVEKLATPDVTIADMIGDVDPIKAAR
                     AGRNLSDELTIHYGLLPRANRGIFAINELPDLAGKIQVGLFNIMQEGDVQIKGYPVRL
                     LLDVLLVFTANPEDYTARGKIITPLKDRIGSEIRTHYPATLAEGMSITEQESWIARDG
                     KRRVEVPTYVREIVEEVAFQARDDKRIDRRSGVSQRLPISALENVLSSAEQRALRSRD
                     SAVVARVADVYAAIPAMTGKFELEYEGEMRGAENIARELIRAAVGKTFTKYFANVNFQ
                     TVVQWFEAGGELKIPANASSAELLGQLKKITGLFEHLEALGVRSKDDAAIQASAAEFI
                     LEGLWSQKRINRSEERGFFAEARKTAESRDPKEPIGRMPRRQFN"
     gene            complement(3621..4244)
                     /locus_tag="VN788_15055"
     CDS             complement(3621..4244)
                     /locus_tag="VN788_15055"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HXU21899.1"
                     /translation="MERPARIPLFPLDVVLLPRMQFPLHIFEPRYKVMISRCLEEKIE
                     FGMILAANNNVATMGCTAEILRKTKDYSDGRMDIATEGRAVFRLVDLLHEKEYYEGIV
                     EYAKDELVTLDPAKERQLVIAFEQSQVLLFGRPWTDPSPNDPGTLAYRMAAMVPMEIE
                     KRQTLLQTSSEQERREIVLDWLQAFVPKLAEQQSARRRAGGNGHPVN"
     gene            complement(4274..4597)
                     /locus_tag="VN788_15060"
     CDS             complement(4274..4597)
                     /locus_tag="VN788_15060"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU21900.1"
                     /translation="MAQMQKTRREILEEFVAQKPTDAFARYGLALECVKLGDSSAAAT
                     HFDNLIATNPDYIAGYYQFGQLLGRLGRLDEARKLLSDGIVVAQKAGDMHARDEMQAA
                     LTLLR"
     gene            4841..5785
                     /locus_tag="VN788_15065"
     CDS             4841..5785
                     /locus_tag="VN788_15065"
                     /inference="COORDINATES: protein motif:HMM:NF022818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:5030..5032,aa:Sec)
                     /transl_table=11
                     /product="DUF3179 domain-containing (seleno)protein"
                     /protein_id="HXU21901.1"
                     /translation="MLPYTTVDHPTFVPASQASFLSDTDVVIGVSSGGVAKAYPGAEL
                     AQHGVVQDKMPDGPIAVTWUGYCNTALVIRAEANGKPLHFDTKGVVGLNEVFKDRETG
                     SLWQQSLSTATSGPLKGTHLGLYPFLLTQWGEWRKQHSDTLVLKPLPGYAERMPAENK
                     FINQQWSAGISDSRPAPKGAIGHDTRLGPREIVLGLQVNHGEKAYPLSALRKQSVIND
                     TIGGAPTVVIHQPASDTTTAFDSTLKGKVLKFKAADTEADRLVDLQTHSTWNAYGLCA
                     SGSLKGSQLKPLILEPEFWFAWSEFHPKTEVYSVSSHR"
     gene            5874..6317
                     /locus_tag="VN788_15070"
     CDS             5874..6317
                     /locus_tag="VN788_15070"
                     /inference="COORDINATES: protein motif:HMM:NF012417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU21902.1"
                     /translation="MNTALGAAVATLLSGTVASLRAESALPQQDSQGTTPAKPPRGVA
                     TLMTESIGDPGDCEGRMIVLTIPPKNVSAPHKHPGPVFAYILEGKIENQVDPEPPKTY
                     SAGDVFYEPALHVHRSLRNLSDTETARVLVFQLVPKGQPGVLPAK"
     gene            6623..7519
                     /locus_tag="VN788_15075"
     CDS             6623..7519
                     /locus_tag="VN788_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21903.1"
                     /translation="MVCSTPNTRGLILRSLVFLSATAAALVSMPRMTSAQHTAGHTVA
                     EPTHPVVIARGAYRPPASASRPVPVSRPARAIVPVPMARPVAPSRPARSTYPVRVTTP
                     LPGLRVIEPTHVAGRNSFRAPRFVVPFFFTASIPESSGLAGSVSQFGGLPLGFGLWPA
                     CDSATTPGRFWTVGPCFGLGSYSAELTPAPAQTGPYIPPLTLFESAPPSGQAAAQPPS
                     GPAPTTTRVLYFKDGTTIAATDWWVSLGRLQYITDSGQTGSVDVMQLDLERTNKENQS
                     RGLDFRLKFTPPSDLYPPSIRP"
     gene            7631..8251
                     /locus_tag="VN788_15080"
     CDS             7631..8251
                     /locus_tag="VN788_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012404383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HXU21904.1"
                     /translation="MPGNPRSGRPDPGHPEFPPTIGSTAETLIAAKVPARANNTAHNA
                     SPDGPSIEGAVPISLRINRKDHQLFVDPRTTLLDCIREAVVLTGTKKGCDHGQCGACT
                     VHVNGRRVVSCLSLALMHDGEDITTIEGIGTPAALHPMQSAFLACDGYQCGYCTSGQI
                     MSAVALLKEPCGPDDAAVKELMSGNICRCGAYANIIAAIQQVRRSS"
     gene            8274..9275
                     /locus_tag="VN788_15085"
     CDS             8274..9275
                     /locus_tag="VN788_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002715318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HXU21905.1"
                     /translation="MRTFEFMRAEDAATAINTAALAKTAQQGADVRFVAGGTTLIDLM
                     KLDVETPARLIDINRLPLDKLEEMPGGGLKIGATVRNSDLANDPRVQRDYAVLSQAVL
                     AGASAQLRNMATTAGNLLQRTRCMYFRDTAMPCNKREPGTGCPAITGSNRMLAILGTS
                     EHCIATNPSDMCVAMAALEATIHVQGTKGARSVPIAEFHLLPGSTPHRETVLEPGDLI
                     THVTLPPPRSGSGQVYLKLRDRASYEFALASAAVVMNLADQNVSYVRIALGGVGTKPW
                     RSPEAEAELVGKPANAANFRKAAEAALRNAKPQSENKFKIELSKRCLAHALETAAKG"
     gene            9295..11550
                     /locus_tag="VN788_15090"
     CDS             9295..11550
                     /locus_tag="VN788_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007746248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HXU21906.1"
                     /translation="MATTQESVVSPIGRDTPRVDGLLKVSGQAQYTSDFHFPGMLYAV
                     PIGATIANGKLVKLDTSAAEKMPGVRAILHRKNIGKIFRSTRQQGFAGICEERRPPFE
                     DDTIYYYGQYIALAVADTFETAKAAADAVRATYAMEKPNVATDLEADDDPDLVVGTFG
                     PRHRLESQRGDADAAFATAPVKLDQTYVTPSETHNVLEPHATTAIWDGPILTLYEPSQ
                     GVVNLRSVLAQMFGLPNENVRVITKFVGSGFGNKLWPWTHCPLAAAAARQLGKPVRLV
                     LSRKMTFFAVGHRPRTQQRVRIGATPDGKLVSLHHDYIYQRSILDAHHEDCGEATPSQ
                     YSVPNLRVTFGRAKRNIGAGADMRGPGTVAGLYATESAMNELADQLNIDPVKLRIMNE
                     PKIDESLGIPFSSRHLVECFQVGSERFGWSKRTPGVGSMKRDGLIVGWGMAGCSWVAA
                     RFGAAANLQLRDDGTCRIASATQDIGTGTYTILAQLASQRTGVPLDKVEVELGDTSLP
                     EGPVSGGSMVTGSVVPAVFAAADGAIASLMMTAASTPGSPFVNRRPQYLGFANGQVFV
                     KAEGPAKGVPFAEILRLANLRLVTGNGKSESTFGNPKPKFSTHSCGCHFAEVTWQPEI
                     ARLRVSRVVTVIDAGKIINPLAGRNQIQGAVVMGIGMALLEHTSYDPQNGAPINSSLA
                     DYIVAVNADVPPIEVHFLDYPDKEINEVGARGIGEIGLTGIAAAITDAVNHATGVRVR
                     ELPVKIENLLM"
     gene            complement(11564..12934)
                     /gene="hemG"
                     /locus_tag="VN788_15095"
     CDS             complement(11564..12934)
                     /gene="hemG"
                     /locus_tag="VN788_15095"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HXU21907.1"
                     /translation="MASSAQVVVIGGGISGLACALRLQQLGAPVTLLEANDHPGGLIG
                     SVERDGFLFESGPQSFQGTETLLGLIRDLGIEKDLCKADPRAPRFVLRHGRLRKIPMA
                     PQGILTSSLLGVGSRWRIVSEGLGRTAPPDEEESVAQFVRRKFGHEILEYLVSPFVSG
                     VYAGDPEKLSMRAAFPSLEEWEQQYGSVLRGAMKSRPAKGARQGPPPLCSFQHGMGVL
                     TEAMFAKLGANAKSGARVEAMTRAEQGFQIQVTQSGRSEQMNARAVVLATPAYVASHI
                     AAPLSAKAAQILSGIAYAPVAVVAAGYHNRQSPATLSGFGVLIPRSEKYRTLGIVWNS
                     SLFPNRAPQGQMTITSILGGATDSAIIEKSEEEITAIAVHEHSKILRIEGSPIISAIW
                     KYPRALPQYNLGHAHAVREIREVERAIPGLYFAGNYLEGPSIGKCVEQGFHTAGAVRG
                     DLKKLL"
     gene            13230..14213
                     /gene="ispH"
                     /locus_tag="VN788_15100"
                     /gene_synonym="lytB"
     CDS             13230..14213
                     /gene="ispH"
                     /locus_tag="VN788_15100"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HXU21908.1"
                     /translation="MTLTNIQGTASGVKTLVRVRPRGFCAGVVRAVDIVELALEAYGR
                     PVYVHHEIVHNRYVVDQLRRRGAIFVESIEEVPVGAVLVFSAHGVPPRVRSEAKERDL
                     KVIDATCPLVTKVHLEALKFAREGRTLILIGHRDHQEIVGTSGEAPHQTLVVGSVEEV
                     DQLQVPDPSKLAFLTQTTLSLYDTQEIVARLRQRFPSIVGPASDDICYATQNRQEAVE
                     ALTREVELILVVGSANSSNSNRLVEVAQRAGVAARLIEDANDIDAASLEGVKTIGLTA
                     GASAPEVLVEQVSQRLSGFGFTNHRDLDLIREDVRFTLPAELAARLGRTTN"
     gene            14268..15404
                     /locus_tag="VN788_15105"
     CDS             14268..15404
                     /locus_tag="VN788_15105"
                     /inference="COORDINATES: protein motif:HMM:NF012703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HXU21909.1"
                     /translation="MAAKIVRQANKIAFLGAPTSAAAMSLGNEGAPAALRAAGLIERL
                     GEIGYQVSDLGDDAPQLFQPDNESPRARNVPRILAALDALKPRVEQAVKSTALPLILS
                     GDCSVALATVAGVRRYFRHAGMIYMDRDADLNTPATTLSGCVDGMVVSHLTGRGAAEL
                     VRFWPEPPLVRDPDLALFGVARLDPAEEDFLRSALLRHYHAAEVRRMGPEAAAEIAVD
                     RIHGKTNEFVLHFDVDVIGGFEATNFSGAGGLNLEEVRGALEVFAAQPHLAAIEVTGY
                     NPAKDAGASGAKLIVELLVGALAKRLAALTAPEPAESANAPVVQTSAEPAEEAATPAV
                     TPGEAWSSDDLVSPEESPDNPAGGESSAEASEPLSSEAEERNSQ"
     gene            15448..16188
                     /locus_tag="VN788_15110"
     CDS             15448..16188
                     /locus_tag="VN788_15110"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HXU21910.1"
                     /translation="MTAHPEIIVFDVDGVLVDVRGSFHRTVLDTVRHFTGKRVTRLEL
                     LRWKSQSGFNDDWKLSTAWVQSLGGKFEYAEVKQKFIEFYWGSGGEGYVSREKWLLPR
                     SSLRRLAARSELAIFTGRVRAETDYTLDRRKVREFFRTIVTVEDVERPKPHAEGLLRI
                     LAGRDPSKAIYLGDNVDDAIAAQSAQIPFVGVLAREGAARQERVALLTKFGARAIIND
                     VRDLERWLRVAVSGAIRARQALPRSSTS"
     gene            16317..>16919
                     /locus_tag="VN788_15115"
     CDS             16317..>16919
                     /locus_tag="VN788_15115"
                     /inference="COORDINATES: protein motif:HMM:NF036524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HXU21911.1"
                     /translation="MFKNSLAARANKATITERKILDAALGMFRTKGFDEATMRDVATA
                     AGVATGAAYYYYPSKDAIVLAFYERSCVEMQPRIEEAVAKSAGSLEERIKALIHVKLE
                     YFAPYRGVLRALLKNGADPAHPLSPFSRNNKSIREVDIAWFRKILNDCGVRVPQDLAH
                     YLPDVLWMFQMGVIYFWVTDDSHGRHRTDRLLVLGAKAVTF"
BASE COUNT         3489 a         5013 c         4812 g         3605 t
ORIGIN      
        1 ggccgccagt acgtgctcgg caatgtttac tacggattta gcccctcgca tttctggctc
       61 cgagtggatc ccattcccga ggccatcgca caaatggcgg attttcaaat gcgtatcacc
      121 gtttgggatt cgcgcgagac gcgcatcact ctgcgaatcg acaacgagcg cctcgcgcca
      181 ggttccgtcc tcgaacaggc tggagtgtgt ctgttgcatc ccgaagcagc gatgtcagct
      241 gcttacgaaa aaattctgga gctaagcctc gcccgcgaac tcttcgacct ccgtggccgt
      301 cgcgacctgc tggtaagcgt cgcgctgtgg aaaggcggcc tacctgtcga tgtcctcccc
      361 gccgaaggcg ttctgcaagt ccccctcggc gaagaaaact tcgcctggcc ccctgcgtag
      421 ttttcgcggt cgtcattctg tctctgacga attcggctac gtcaagccac gtgactacac
      481 cacagcggtg ctacgccact taaccttttc gcgatatcag cgaacctgca aaatgctaag
      541 cggtgtctgc gtctagtgaa tgttccgcgt cttgcgccca acgtaatcca tcaagaggta
      601 ttgcccgagg gtataaggag tggtgaagta cgctttgccg cggcaaagct cggtgatctt
      661 ctgcacaaaa tgaaccaaac tgtaatcgct cgccagcata aaagtattga tcaaaattcc
      721 cgcgcgcttg cactttgcca cttcttccat cgtctgcgtc accaccagag gatcgagccc
      781 aaacgcgttt ttgtaaatcc gcccgtcgtc gagcgtcagc gcggagggct tgccgtcggt
      841 gatcatcacg atttggcgca tatctttctt ctggcgcgcc agaattctct gggccattcg
      901 caagccctcg cgtgtattcg tgtaatacgg acccacttgc acgcgcgcca attttcccag
      961 cggaatttct tccgcgctgt cgtggaaaag cacgcagtgc agggaatcgc ccggatattg
     1021 tgtgcggatc aagtgcgaaa gcgccagcgc cactcgcttc gccggcgtaa accggtcttc
     1081 accgtagaga atcatgctat ggctgcaatc cagcattagc actgtcgcgc acgagctttg
     1141 atactcgcac tgatgcacca ttaaatcggg atagtccagt tcgatgggaa ttcccgcgcc
     1201 attgcgccgc accgcgttga agagcgttcc gctgatgtcc atattcagcg tgtcgccaaa
     1261 ttcataggcc ttcgacgagc cgcccgactc gatgcccgtg gccagatcgc gcgtgtcgtg
     1321 ccgcccaaag ctgcttttcc cgagcgacgc cagcaaatcc ttcagcgtct tgaatcccag
     1381 aaaatcgagc gccttgtcgg tgatttcaaa tcgcgcttcc acctgcggct ggtcttttcc
     1441 gatctgtcct ccgggcgttt ccgaaggcgg aggagtcacg cgtgcggatt gctgctgcgt
     1501 gatgaagccc tcctcctcca tacgctgaat gatctggtca atcatgttgc gcagctcttc
     1561 gttctgcgaa agatcgggat tctgccgcag cttctcgagc aactctggcg gaatcagatc
     1621 accgtcttcg agagcgcgca gaatcgcttc ccgcagctcg tccatgtgct gctggcgagc
     1681 ccgctccata tccagttcat ggaaaccgta ttgtgagtgg aatccgcttt gaaggaaaaa
     1741 atccgaaagt cgatccatca ggtcttgcag atcaatgtca tccgccgctc ccggttcata
     1801 gcgcgaatat ttcgtgtatt tcatttttcc tcagcgaact caattccaaa tcagttgaat
     1861 tgccgccgcg gcatgcgccc gatgggttcc ttgggatcgc gggactccgc tgtttttcgc
     1921 gcctcggcga aaaacccgcg ctcctcgctg cgattgatgc gcttttgcga ccacaggcct
     1981 tcgagaataa actccgccgc gctggcttga atcgccgcat cgtcttttga acgcacaccc
     2041 agcgcttcca gatgctcgaa gagtcccgtg attttcttca gctggccgag caattctgcg
     2101 ctcgaagcgt tcgcgggaat cttcagttcg ccgcccgctt cgaaccactg caccaccgtc
     2161 tgaaaattca cattcgcgaa gtatttggta aacgtcttgc ccacggcagc gcgaatcagt
     2221 tcccgggcaa tattttcggc gccgcgcatc tcgccttcat actccagctc gaattttccc
     2281 gtcatcgccg ggatcgcggc gtagacatcc gccacccgcg ccaccaccgc ggaatcgcgg
     2341 ctgcgcaacg ccctttgttc cgcgctgctc agcacattct cgagcgcgct gatcggcagc
     2401 cgttggctca cacccgatcg ccggtcgatt cgtttgtcgt cgcgcgcctg aaaagccact
     2461 tcctccacga tctctcgcac atacgtgggc acttccactc gtcgcttgcc gtcgcgcgct
     2521 atccacgatt cctgttctgt aatcgacatt ccttcggcca gcgtcgccgg ataatgcgtc
     2581 cgaatttcgg aaccgatccg atccttcagc ggcgtgatga tttttccccg cgccgtgtag
     2641 tcttccggat tcgcggtgaa gaccagcagc acgtccagca gcagcctcac cgggtagcct
     2701 ttaatctgca cgtcgccttc ttgcatgata ttgaagaggc ccacctgaat ttttcccgcc
     2761 aggtccggca gttcattgat ggcgaagatt ccgcggttcg cgcgcggcag cagaccgtaa
     2821 tgaattgtca gttcgtcgga aagattccgt ccggcgcgcg cggccttgat cggatccacg
     2881 tccccgatca tgtccgcgat ggtcacatct ggcgtggcca gtttctccac gtagcgcagc
     2941 tcccgcgaga gccaggcgat cggcgtgcca tccccttccg cggcaattcg ctcgcggcac
     3001 gcgcggcaca gcggacggta tggattgtcg tttatctcgc accctgcgat caccggtatc
     3061 tgcgggtcga ggaatccagc cagccctcgc aggattcgcg acttcgcctg tccgcgcagg
     3121 cccagcaaaa taaaatgatg ccgcgaaagg acggcattgg tgatttgcgg tatcaccgtg
     3181 tcgtcgtagc cgtgcacacc cggaaaaagt ggctcgtcct tttccatttt ttgaagcaga
     3241 ttctcgcgaa gctcctggcg gatggttcgc cccgcgagtt tttcctcgct ccaggaactg
     3301 cgtttcaact cacccagatt ttttggttga ctcattcgat caccggtcgg cacttcaggc
     3361 gcgatggaaa tttcatcgcg cggctccatt atagaggaag cgagcgcacg ctcctaactt
     3421 ggattggtgg aagccgcggc aagttgccaa agctcttacg aatcgacaat cagggcttcc
     3481 agcggccgtc atcctgagcg gagtcccgcg cttttgcttc tccccgcggt ttttgcgggc
     3541 gcgggacgca atcgaaggat ctctcttgga tgtttgaatc ttgctccgcg ctccatcgac
     3601 gagagattgt tcgacgacgg tcaattcacc gggtgcccat tccctccagc gcgtcggcgc
     3661 gcgctctgct gttccgccag cttgggcacg aatgcttgca gccagtcgag cacgatctcg
     3721 cggcgctcct gctcgctact cgtttgcagc aacgtttgtc gcttttcgat ttccatgggg
     3781 accatcgccg ccattcggta agcaagcgtg ccaggatcgt ttggagacgg gtccgtccac
     3841 ggccttccga aaagaagcac ttggctctgc tcgaacgcaa tcacgagctg tctttctttg
     3901 gccgggtcca aagtgaccaa ttcatctttc gcgtattcga caatcccttc gtagtattcc
     3961 ttctcgtgca gcaggtcgac gagccgaaag acggcgcggc cttccgtggc aatgtccatg
     4021 cgcccgtcgg agtagtcctt ggtcttgcgg aggatttctg cggtgcagcc catggtggca
     4081 acgttgttgt tcgccgccag gatcattcca aattcgatct tttcctccag gcatcgggaa
     4141 atcatcacct tatagcgcgg ctcgaaaatg tgcagcggaa attgcatgcg cggaagaaga
     4201 actacgtcca aaggaaacaa tggtattcgg gctggtcgtt ccatttgagg gcgatcccgt
     4261 ctaggtggcg cggttagcgc agtagcgtca aggccgcttg catttcgtcg cgcgcgtgca
     4321 tgtcgcctgc cttctgcgca accacgatgc cgtccgataa aagtttgcgg gcctcgtcca
     4381 gccggcccag tcgtcccagg agttgcccga attgatagta gcctgcgatg taatccggat
     4441 tcgtcgcgat aaggttatcg aagtgagtgg ccgccgcgga gctatcgccc aattttacgc
     4501 attcaagcgc caggccgtac cgcgcgaatg catctgtcgg tttctgggcg acgaactcct
     4561 ccagaatttc gcggcgcgtc ttttgcatct gagccattct cttcgcgtat tatagccgcc
     4621 ggtgttgtgc tcgccaagcg cccagacgtc cggcgtggcc catcattcgg cgcaaccttg
     4681 gctcttctca taagccttcc aattgtgtaa gagtaagggc atgaaaagag tcagattgga
     4741 ggagagtgcc atgacaatgt catggattcg gaacgtcacc atatttgcgg tcggtctcat
     4801 cgtttcaagc tcggcgtggg cgcaaaacaa agaaccgaaa atgttgccgt acacgactgt
     4861 cgatcacccc acattcgtcc cggcttcgca ggcctcgttt ctttcggata ccgatgtcgt
     4921 aattggagtt tccagcggcg gcgtggcgaa ggcctatccc ggcgcggaat tagcacaaca
     4981 cggcgtcgtg caggacaaaa tgccggacgg tccaatcgcg gtcacgtggt gaggctactg
     5041 caacacagcc ctcgtgatta gggctgaagc gaatggaaag ccgctgcact tcgataccaa
     5101 aggggtcgta ggtctaaacg aagtgttcaa ggatcgcgaa acaggaagtc tttggcagca
     5161 atcgctctcc accgccacat ccggtccgct taaaggcacc cacttgggac tttatccgtt
     5221 cttgcttact caatggggcg aatggagaaa gcaacactcg gatacgctgg tgctaaagcc
     5281 gctgcccggt tatgcggagc ggatgccagc cgagaacaag ttcatcaatc agcaatggag
     5341 tgccggcatc tcggacagcc gtcccgcgcc taaaggagcc atcggacacg atactcgtct
     5401 cggtccgcgc gaaatcgtcc tcggtcttca agtgaatcac ggagagaagg cctacccact
     5461 ctcggcgctg cgcaagcaaa gtgtgatcaa tgacacgatc ggcggtgcgc cgaccgtcgt
     5521 tattcatcag ccggcgtccg acaccacaac cgctttcgat tcgacgctca aaggcaaagt
     5581 tctcaagttc aaggcagcgg acactgaagc ggatcgcctc gtcgatctgc aaacgcattc
     5641 cacctggaac gcatacggac tctgcgcctc gggaagtctc aaaggctcgc aattgaagcc
     5701 cctcattctc gaaccggaat tctggtttgc ctggtccgaa tttcatccaa agaccgaggt
     5761 ttatagtgtc tcgtctcatc gttagttcgt catcacagcc cggcgctcaa aatcgcggcc
     5821 gagcctcgtt gactatctcg aaaggacatt aagccatgat cactcgccgt gaaatgaata
     5881 ctgctttggg cgccgcagtc gcgactctgc tctcgggaac cgtcgcctca ctgcgcgcgg
     5941 aatcagctct accgcagcaa gattcgcaag ggactacgcc ggcgaagcct ccgcgcgggg
     6001 tggcgacctt gatgactgag tccatcggtg atccaggcga ttgcgaagga cgtatgatcg
     6061 tgcttacgat cccgcccaaa aacgtttcgg ctccgcacaa gcatcccggc ccggtattcg
     6121 cgtacattct ggaaggtaaa atcgaaaatc aggtcgatcc cgagccgccc aaaacctact
     6181 cggccggtga cgtcttctat gaacctgcgt tgcacgtcca ccgctcgctt cgaaatctga
     6241 gcgacacgga gaccgccaga gtactggttt ttcaattggt tccgaaaggc caacctgggg
     6301 tccttcccgc aaaataagtc accgtttgcg ctgtcaaaat gcacgttcct ctccgggtcg
     6361 gttccagacc actgaaattc ttcggcgatg tctgaaagcc agtggagcgc gatatcggaa
     6421 tcctatcgcg cgaccgctct cttctatcag ccgcgatagt taagcctacg cacaactcat
     6481 caacctcttt gataggttga acgaggagtg ctggcggcag ctcgccgcac aggtagtcgc
     6541 atcggtattt tttctccgca caaaaatgtc gcttgcacgt gttgatccaa aagaggatgc
     6601 taaaaaaagg tgggaggcgg agatggtttg ctcgacgccg aacacgcggg gcttgattct
     6661 gcgctcgttg gtgtttcttt ccgcgactgc cgcggctttg gtctcaatgc cgcgcatgac
     6721 aagcgcgcaa cacaccgccg gccataccgt ggctgagcct acgcatcccg tcgtgatcgc
     6781 cagaggagcg tatcggccac cggcatccgc atcgcgtccg gtgcccgtgt cgcgcccggc
     6841 gcgtgcgatc gttccggtgc cgatggcgcg gcccgtcgcg ccctcaaggc cggcccggtc
     6901 cacatatcca gtgcgggtaa cgaccccgct gcccggtttg cgcgttatcg agcccaccca
     6961 cgttgctggg cggaactctt tccgcgcgcc gcgtttcgtc gtgccgtttt tcttcaccgc
     7021 gtcgattcca gagagctccg gtctcgcggg ctccgtttcg cagttcggag gtttgccgct
     7081 gggattcggc ctctggcctg catgcgattc cgcgacaacg ccgggtcggt tctggaccgt
     7141 ggggccgtgt ttcgggttgg gttcatactc cgcggagttg actccagcgc cggctcagac
     7201 tggcccttac attccaccac tgacgctttt cgagagcgcg cctccgtctg gccaggccgc
     7261 ggcgcaacct ccgagcgggc ccgctccgac aacgacgagg gtcctgtact ttaaggatgg
     7321 cacgaccatt gcagccacgg attggtgggt cagtcttggg cgccttcagt acatcaccga
     7381 ctcagggcag acaggctccg tggacgtaat gcaactcgac ctagagcgca ctaacaaaga
     7441 aaatcagagt cgcggactgg acttccgtct caaattcacg ccgccttcgg acctctatcc
     7501 cccgtctata cggccctagc gcttgcacgg tcgtttgcga ttacattcga cgctggctcg
     7561 ctcctcacca cgtacggtgg acagtttgtc gttgcttcga tagacttttt aagtcttcaa
     7621 cggaggcccc atgccaggta atccgcgatc gggccggcca gatcctgggc accccgaatt
     7681 tcctccaacg atcggaagca cagcagaaac gcttattgca gcgaaggtac cagcgcgcgc
     7741 gaacaacact gcccacaatg ctagtccgga cgggccgagc atcgagggtg ccgtccccat
     7801 ctcgttacgc atcaatcgca aggaccatca actttttgtc gaccctcgga caactctgct
     7861 ggattgcatt cgtgaggctg ttgtcctcac cgggacgaaa aagggttgtg accacggtca
     7921 atgcggcgcc tgcactgtgc atgtgaacgg gcggcgagtg gtttcctgct taagcctggc
     7981 actaatgcac gatggcgaag acatcacaac gattgaaggc atcggcacgc ctgccgcgtt
     8041 gcaccccatg cagtccgcat tccttgcttg tgacggctac cagtgtggat attgcacgtc
     8101 cggtcagatc atgtcggcgg ttgctctgct caaggagccg tgtgggcccg acgatgcagc
     8161 cgtaaaggaa ttgatgagcg gtaatatctg ccggtgcggg gcatacgcca atatcatagc
     8221 tgcgatccag caagttcgca gaagttccta gccacgagaa agagctaatt tccatgcgca
     8281 ctttcgaatt catgcgggcc gaggatgccg ccacggcgat taacacagcc gcactggcga
     8341 agactgctca gcagggtgcg gacgtgaggt tcgtcgcggg cggtacgaca ctgatcgatc
     8401 tgatgaagct agacgtcgaa acgcccgcgc gactgatcga cattaatcgt ttgccgctcg
     8461 acaaactcga agaaatgcct gggggcggac tcaagatcgg cgccacagtc cgaaattcag
     8521 atcttgcgaa tgatcctcgc gtacaacgag attatgccgt gctgtcgcaa gctgttctcg
     8581 ccggcgcatc ggcacagctt cgcaacatgg caacgaccgc cggcaacttg ctccagcgaa
     8641 cccggtgcat gtatttccgc gacactgcga tgccgtgtaa caaacgcgaa cccggcactg
     8701 ggtgcccagc catcaccggc agtaaccgca tgctcgcaat cctgggaaca agcgaacatt
     8761 gcatcgccac aaacccatca gacatgtgcg tggcgatggc tgcattggag gcaaccatcc
     8821 atgtgcaggg aacgaagggc gcacgttcgg ttcccatcgc tgagtttcat cttctgcctg
     8881 gcagcacgcc gcaccgcgaa accgtgctcg aacccggcga cctcatcact cacgtcacgc
     8941 tcccgccacc cagatcgggc agcgggcagg tctatctgaa gttgcgcgat cgggcgtcgt
     9001 atgaatttgc tctcgcgtcg gcggctgtgg tgatgaatct cgctgatcaa aacgtcagtt
     9061 atgtgcgcat cgcgttgggc ggcgtaggca ccaaaccgtg gcgttctccc gaggcggaag
     9121 ctgagctcgt tggcaagccc gctaacgcag caaacttccg caaagccgcc gaagccgccc
     9181 tgcgcaacgc caaaccgcag agcgagaaca agtttaagat cgaactatcc aagcgctgtc
     9241 ttgcgcatgc tttagaaact gctgcgaagg gttgacccta tccaaggaac gagtatggcg
     9301 acaacacaag aatcagttgt ttctccgatc ggccgtgaca cgcctcgagt agatggcctc
     9361 ctcaaagtga gcggacaggc gcaatacact tccgacttcc actttccggg gatgctatat
     9421 gccgtgccga tcggggcgac catcgccaac ggcaaactcg tcaagctcga tacgtccgcg
     9481 gcggagaaga tgcccggtgt gcgcgcgatc cttcatcgca agaatatcgg aaagatcttc
     9541 cgctcgaccc ggcagcaggg atttgcaggc atttgcgagg agcggcgccc accgttcgag
     9601 gacgacacca tttactatta cgggcaatat attgcgctcg cggtggcgga cacattcgaa
     9661 accgccaaag ccgctgccga tgcggtgcga gcgacctacg cgatggaaaa gccgaacgtc
     9721 gcgactgacc tcgaagcaga tgacgatccc gatttggttg tgggcacgtt cggcccgcgg
     9781 caccgtttgg aaagtcagcg aggcgacgcc gacgctgctt tcgcgaccgc gccggtcaag
     9841 ctcgaccaga cttatgtcac gccgtccgag acgcacaacg ttcttgagcc gcatgccacc
     9901 accgctatct gggacggacc aatcctgacc ctttacgaac cctcgcaagg agtcgtcaat
     9961 ctgcgaagtg tcctagcgca aatgttcggt ctaccgaacg agaatgtgcg cgtcatcacc
    10021 aagttcgtgg gctccggttt cggcaacaag ctatggcctt ggacccactg tccccttgcc
    10081 gccgctgcgg cgcggcaatt aggcaagccg gtcagacttg ttctcagccg caagatgacc
    10141 ttttttgcgg tcggtcatcg gccgcgtacc caacagcgtg tgcgcatcgg tgcgactcct
    10201 gacggcaagc tcgtttccct ccaccacgac tacatctacc aaaggtccat actcgacgct
    10261 catcacgaag attgcggtga ggctacgccc tcccaataca gcgtgcccaa cctgcgcgtg
    10321 acgttcggcc gagcgaagcg aaacatcggc gccggggctg acatgcgcgg tccaggcacg
    10381 gtcgcggggc tgtacgccac tgaatcggct atgaatgaac tggccgatca actcaacatc
    10441 gacccggtca aacttcgcat catgaacgag ccgaaaatcg acgagagctt gggtatcccg
    10501 ttttcatcac gccatttagt tgagtgcttc caagttggct cggaaaggtt cggttggtcc
    10561 aaacgtaccc ccggcgtggg ctccatgaag cgcgacggcc tgatcgtggg ctggggaatg
    10621 gcgggatgct cgtgggtcgc ggcacgattc ggcgccgcag ccaacctaca attgcgtgac
    10681 gacggcacct gtcgaatcgc aagcgccacg caggacatcg gcaccggcac gtacacgatc
    10741 ctggcgcaac ttgcgtccca gcggaccggc gtgccgctcg acaaagtgga agttgaactc
    10801 ggtgatacct cgcttccgga agggccggtc tccggcggct cgatggtcac cgggtcggtg
    10861 gttccagcgg tatttgcggc ggccgatggt gcgatcgcat ccctcatgat gaccgcagcg
    10921 tccactccgg gatcgccctt cgtgaatcgc aggccccagt atctggggtt cgcgaatggt
    10981 caagtcttcg tgaaggctga aggtcctgcg aaaggcgtac ccttcgcaga gattctacgg
    11041 cttgccaatc tccggctggt cacaggcaac ggcaaatcgg agtcaacatt cggtaatcca
    11101 aagcctaaat tttcgacgca ctcctgcggt tgtcactttg ctgaggtcac ttggcagccg
    11161 gaaattgctc gcttgcgggt tagccgcgtc gtcaccgtca tcgatgccgg caagatcatt
    11221 aacccactgg cgggccggaa ccagatccaa ggggcggtgg tgatgggcat cggcatggca
    11281 ctgcttgagc acacttcgta tgatccccag aacggtgctc cgatcaacag cagtttggcg
    11341 gactatatcg tcgcggtgaa cgcagacgtt cctccgatcg aggtgcattt cctggactat
    11401 ccggacaaag agattaatga ggtcggcgcg cgcggaatcg gtgaaatcgg tctgactggt
    11461 atcgcagcag ccatcacaga cgcagtgaac cacgctacag gtgttcgcgt gcgtgaattg
    11521 cctgtaaaga ttgagaattt gctaatgtga gtcgccctcc cagttacagc agtttcttca
    11581 aatctccgcg cacggccccc gccgtgtgaa agccttgctc gacgcatttc ccgatcgacg
    11641 gcccttcgag gtaattgccc gcaaaataca ggcccggtat ggcgcgctcg acctcgcgga
    11701 tttcccgcac ggcatgcgcg tgccccaaat tgtactgagg caaagctcgc ggatatttcc
    11761 agatggccga aataatcggc gagccttcaa tcctcagaat tttagagtgt tcgtgcaccg
    11821 cgattgcagt gatctcttct tcggatttct cgataatggc ggaatcggta gctccgccga
    11881 gaatgctcgt gatggtcatc tggccttgcg gcgcgcgatt cggaaataaa gacgaattcc
    11941 acacgatgcc gagcgtccga tatttttcgc ttcgtggaat cagtactccg aagccgctta
    12001 gtgtcgctgg gctttgacgg ttgtgatatc cagccgcgac caccgccacc ggcgcgtacg
    12061 cgatgcccga aagtatttgc gccgccttcg ccgaaagcgg cgccgcgatg tgcgatgcga
    12121 cataggccgg cgtcgcaagc accactgcgc gcgcgttcat ttgttcgctg cggccgctct
    12181 gtgtaacctg gatttgaaaa ccctgctcgg ctcttgtcat cgcctccacg cgtgcaccgc
    12241 tttttgcgtt cgctccaagt tttgcgaaca tcgcctcagt cagcacgccc attccgtgtt
    12301 gaaaggagca cagaggcggc ggaccttgcc tggcgccttt tgccggacgc gacttcatcg
    12361 ctcctcgcaa aacgctcccg tattgctgct cccactcttc gagtgaagga aacgcagcgc
    12421 gcatgctcag cttctccggg tcgcccgcat aaacacccga aacaaacggt gacacgagat
    12481 actccagaat ttcatgtccg aactttcgcc gcacaaactg cgcaacagat tcttcctcgt
    12541 ccggtggcgc cgtccggccc aatccttcgg aaacaattct ccatcgcgaa cccactccga
    12601 gcagggaact ggtgaggatg ccttgcggcg ccatcggaat ctttcgcagc cgtccgtgcc
    12661 gcaaaacaaa tcgcggcgct cgcggatcgg ccttgcacaa atctttttcg atgcccaagt
    12721 cgcggatcaa acccagcagc gtttcagttc cttgaaaact ttgcgggccc gattcgaaga
    12781 ggaagccgtc gcgttccacg gaaccaatta gtcctccggg atgatcattc gcttcgagca
    12841 atgtgacggg tgcgcccagc tgttgcagcc gcaaagcgca tgccaagccg gaaatgccgc
    12901 ctccgataac gacgacctgg gctgagctgg ccatgtcggc accatcctag tgccttcgga
    12961 acggaaaaac acaccctaag tgtgaccgag tggcccgacg ctccgaaagt ctccgcgagg
    13021 aagggttttg gaggcggttt ttgccgtttt gcgcctcgtt aatgtttcta attgaaaact
    13081 tttcgcgctg caaactagaa gctttccaag ctgcagacta gaagcatttt gggccccgaa
    13141 ttgacacaag tagcgatgga acaaactggc agggaagcta ggcttcaggt aatatcaggt
    13201 aatgttacgg ggcgtaccgg agaaccctat tgacactcac taacattcag ggcacagcaa
    13261 gcggggtaaa aacgctcgtg cgggtgcggc ctcgcggttt ttgtgcgggc gtggtgcgcg
    13321 cggtcgatat cgtggaattg gcattggagg catacgggcg gccggtttac gttcaccacg
    13381 aaattgttca taaccgctac gtggtcgacc agttgcgccg gcgcggcgcg atttttgtgg
    13441 agtcaatcga ggaagttcca gtcggagccg tgctggtctt cagcgcgcac ggagtgccgc
    13501 cgcgtgtgcg cagcgaagcc aaagagcgcg atttaaaagt catcgacgcc acctgccccc
    13561 tggtaacgaa agttcatttg gaagctctga aattcgcgcg cgaagggcgc acgctaattt
    13621 tgattgggca ccgcgatcac caagaaattg tgggaacgtc aggcgaagcg ccgcatcaaa
    13681 cgctcgtcgt tggcagcgtg gaagaagtgg atcaattgca ggttccggac ccgtccaagc
    13741 tagctttttt gacgcagacc acgctctcac tttacgacac gcaggaaatt gttgcgcggt
    13801 tgcgccagcg ttttccgtca attgtcgggc cggcgtcgga tgatatttgt tacgcaacac
    13861 aaaatcgcca ggaagcggtg gaagcgctta cgcgcgaagt ggaattaatt ctcgtggtcg
    13921 gctcggcgaa cagctcaaat tcgaatcggc tggtggaggt tgcacagcgc gcaggagtgg
    13981 cggcacggct gatcgaagac gccaatgata ttgatgcggc gtctctggaa ggcgtgaaaa
    14041 ctatcggcct cacggcggga gcttccgcgc ccgaagtttt ggtggagcag gtgagccaac
    14101 gcctttccgg attcggattc acgaaccatc gggacctgga tttgattcgg gaggacgtgc
    14161 gattcacgct gccagcggaa ctcgcggccc ggctcggcag gaccacgaat taaggatcaa
    14221 cagttttcgc cccgggcaac atcaacccga aggtaaggag catatgagtg gcggccaaaa
    14281 tcgtccgcca ggcgaataag atcgcatttc tgggagcccc cacaagtgcg gctgcaatgt
    14341 cgctgggcaa tgagggcgcg cccgcagcgc tgagggcggc tggtcttatc gagcgccttg
    14401 gcgaaattgg atatcaggtc tcagaccttg gtgacgatgc cccgcagctt ttccaaccgg
    14461 ataacgaaag tcctcgcgca cgaaatgttc ctcgaatttt ggcagctctc gacgcactga
    14521 agccgcgcgt ggagcaagcg gtgaagtcga ctgcgctgcc gcttattctg agcggcgact
    14581 gctccgttgc actggccaca gttgccggcg ttcggcgtta tttccgacac gcgggaatga
    14641 tctatatgga ccgcgatgcc gatctgaata cgccggcgac gacactttcc ggttgcgttg
    14701 acgggatggt ggtttcacat ctcaccgggc gaggcgcggc cgaattggtg cgcttctggc
    14761 cggaacctcc actggtccgc gatcccgatc tggctctttt tggcgtggcg cgtttggatc
    14821 ccgcggaaga ggactttttg cgaagcgcgc tgctgcgtca ctaccatgcc gcggaagtac
    14881 gacggatggg gccagaagcc gcggcggaaa ttgcggttga ccgaattcac ggcaagacaa
    14941 acgaatttgt gctgcacttt gatgtggatg tgatcggcgg tttcgaagcg acgaacttta
    15001 gcggagcggg cggattgaat cttgaagaag ttcgcggcgc gctggaagtg ttcgccgcgc
    15061 aaccgcatct cgcggcgatt gaggtgaccg gatacaatcc ggccaaggat gcaggcgcaa
    15121 gcggcgcgaa attaatcgta gaactcctcg taggcgcgct ggcgaaaagg cttgcagcgc
    15181 tcaccgcgcc cgagcctgcc gaatcggcaa acgcgcccgt ggtgcagaca tccgcagagc
    15241 ccgccgaaga agcagcaacg ccggctgtga cgccgggcga agcttggtcg tccgacgatc
    15301 tggtctcgcc ggaagagtcc ccggacaatc cagcgggcgg ggaatcgagc gccgaagctt
    15361 ccgaaccgct ttccagcgaa gcggaggagc gcaactcgca gtgatcgcca gcagttctcc
    15421 aatctcgtgt tcaaacccgc caaatttttg accgcacatc cggaaatcat cgtcttcgac
    15481 gtggatggcg tgctggtgga cgtgcgcggt tcctttcatc gaacggtgct ggacacggtg
    15541 cggcatttca ccggcaaacg cgtgacgcgc ctggaattgc tgcgttggaa aagccaatcg
    15601 ggattcaacg acgattggaa gctttcgaca gcgtgggtgc aatcgctcgg aggaaaattc
    15661 gaatatgccg aagtaaagca gaagtttatt gaattttact ggggaagcgg cggagaaggt
    15721 tacgtgagcc gcgaaaaatg gctgctgccg cgatcgagcc tgcgccggct cgcggcgcga
    15781 tcagagctcg caatttttac gggacgcgtg cgagcagaga ccgactacac gctggatcgg
    15841 cgaaaagtgc gcgaattttt ccggacaatc gtcacggtgg aagacgtgga gagacccaag
    15901 ccgcatgccg aagggttgct gcggattttg gcaggccgcg atccaagcaa agcgatatac
    15961 ctgggcgaca atgtggacga tgcgatcgcc gcgcagtcgg ctcaaattcc atttgtcggc
    16021 gttttggcca gagagggcgc ggcgcggcag gagcgggttg cgctgctaac gaaattcggc
    16081 gccagggcaa ttatcaatga cgtgcgcgat ctcgagaggt ggctgcgggt tgcggtgtcg
    16141 ggagcgatca gggcgcgcca ggctctgccc aggtcgagta ccagctaaaa agtcccgata
    16201 agccgattta taggagcttg ctcagcgcgc aaaacgtcct cacctgcggg tctaaatatt
    16261 gaacctgttc aaaagactgc ttgacagccg cagccccagg cgcataatag ttgaacatgt
    16321 tcaagaatag ccttgcggct agagccaata aagccacaat caccgaacga aagattctgg
    16381 atgcggcgtt gggcatgttc cgcaccaaag gcttcgatga agcgaccatg cgcgatgtcg
    16441 ccaccgcggc cggagttgcc accggagccg cctactatta ctacccttcg aaagatgcaa
    16501 ttgtgctcgc cttctatgag cgctcttgcg tggagatgca gccgcgaatc gaggaggcgg
    16561 tagcgaaatc agccggcagc ctggaagagc ggatcaaggc cctgatccat gtgaagctag
    16621 agtactttgc gccgtatcgc ggtgtccttc gcgctctgct caagaacgga gccgatcccg
    16681 cgcatccgct atctccgttc tcccgaaaca acaaatcaat ccgcgaggtg gatatagcgt
    16741 ggtttcgaaa aatcctgaac gactgcggcg ttcgcgtccc gcaggatctg gcacattatc
    16801 tgccagatgt tttgtggatg ttccagatgg gagtgatcta tttttgggtc accgacgatt
    16861 cgcacgggcg gcatcgcacc gatcgcctgc tcgtccttgg tgcgaaggcc gtcacgttt
//
LOCUS       DATNDT010000459         7996 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_361725, whole genome shotgun sequence.
ACCESSION   DATNDT010000459 DATNDT010000000
VERSION     DATNDT010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7996)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7996)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7996
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_361725"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..261)
                     /locus_tag="VN788_15120"
     CDS             complement(<1..261)
                     /locus_tag="VN788_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21912.1"
                     /translation="MSSSPVVTVETPESSSAVGRMFGALFSPKETFHSIARRPTWLPP
                     IILACLLALVVVALFSHRVGWRSYLDKQVANSSRFQQLSADQQ"
     gene            complement(348..1625)
                     /locus_tag="VN788_15125"
     CDS             complement(348..1625)
                     /locus_tag="VN788_15125"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXU21913.1"
                     /translation="MTIQQVNERQNATLFTVFAILAAVLYVFYILIVVQIALGLYSLW
                     DGFGWFRMVRGRLSSHAGFYAPLAAVICPCKGIEPGLKENLAALTRFDYPNYEIYFAI
                     ANERDPAAKIAKEVKDAGVRPVHILFAGPPEGCSGKVQNLKKAVESLPENIEVLVFVD
                     SDARLSRGWLAKLIAPLQDVRLGATTAYRWIIPSRSIGSGGFSSALASAWNGSIATLL
                     GRPRENFCWGGGTAIRRKTFEDEGVPEAWKGAVSDDLALTRALEAAGKPILFCAECLA
                     ATTHPWTPASLLEFTNRQMLIARVYSPRRWLMGAAANVSYSLTLIYAVVVVVKTMAGG
                     DPWVQLALMTLLVVLLSAIKGALRTIAVGEMLPEWRDQLNQWSWVWVALAPVVPFLFT
                     WNFIASLLTRRIRWRNIRYELVSPSVTRVLNRY"
     gene            1687..2763
                     /locus_tag="VN788_15130"
     CDS             1687..2763
                     /locus_tag="VN788_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXU21914.1"
                     /translation="MSTPAAQPLNEAKLHEFVMKAVGEMGAAMNAALILIGDKLGLYK
                     AMAGAGPITSAELAAKTKTAERYVREWLASQAAGGFVAYDAATGKYTLPPEQAMALAD
                     EQSPVFLPGFFEIVSACMKDEPKITEAFRNGNGLGWHEHDHSLFAGTERFFRPNYRAH
                     LVSEWIPALGDIEAKLKAGARVADVGCGLGTSTILMAQAFPKSTFVGFDYHPKSIEMA
                     REAAAKAGVADRVKFEVAKAKDYSGKGYHLVAFFDCLHDMGDPEGAARHVLETLDPNG
                     AWMIVEPFAHDKLEDNLNPIGRVYYGASTMLCTPASLSQEVGLGLGAQAGQGRLTKVL
                     TAAGFKRVRRAAETPFNIVLEARP"
     gene            complement(2767..3426)
                     /locus_tag="VN788_15135"
     CDS             complement(2767..3426)
                     /locus_tag="VN788_15135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21915.1"
                     /translation="MPYRPRELVVRAREAAAILLATLDRNEMVARFLDMYAAENRRPG
                     RADQPAFYREQLQTIRRESILATVLRIETALPARLKVRVTVRGAKAPRKKKSGKKSGG
                     LRPTKRKANLELAIPFLDLFREEFFVALGQSLDWTDEDAQEFWHDLELYEKLSGADSR
                     RQSGRTARARTSGPFVDRVALLLDPSLMEQARRAAGKFQLELNAAADRVLRKIFSRHA
                     H"
     gene            complement(3598..6018)
                     /gene="lon"
                     /locus_tag="VN788_15140"
     CDS             complement(3598..6018)
                     /gene="lon"
                     /locus_tag="VN788_15140"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HXU21916.1"
                     /translation="MTTTRVIPGELAILPVRDTVLFPGAVMPLPVGRESSLALLESLD
                     GPEKLLGVVAQLDPRIEIPTGTDLHGVGTLAKVHKMVRMPNGNVVAFLEGLERIRVLE
                     VVGLKPFLRARVEAQPDLENERDAELTALERNVLELFKEVVARSPQLSDDLQTEASNI
                     ETPARLADFVASTLPSLNTMVRQELLETFSVRKRLETLSNELSKEKEVLELRSKIHDQ
                     VQEQVNQSQREFLLREQMKAIQKELGESDDAQSEVEELRKKAEESGMSAEAKKECDRE
                     LKRLAKMTPASAEYMVSRTYLEWMTSLPWNKTSGTGEEQIDIAKAQEILDEDHYDLEK
                     VKQRILDYFAVKKIQPGMKGPILCFVGPPGVGKTSLGQSIARAMGRKFVRISLGGMHD
                     EAEIRGHRRTYIGALPGQIIQGIRRAETLDPVLMLDEVDKLGRDFRGDPSAALMEVLD
                     PEQNSTFRDHYLDVPFDLSKVLFVATANWMDPIPEALRDRMEILELPGYTEEEKIHIA
                     KRFLIPRQTKEHGIKLGEQIEFSDETLHDLIHHYTREAGVRNLEREIATLVRKQARRI
                     AEGKTDKLTVTPEVIREALGIPKFRLEKEVEERVQKPGVAVGLVWTPVGGDIVFIEAS
                     KMRGGKQFTMTGHLGEVMQESMRAALSWVRANSERYGIDADFFRKLDLHIHVPSGAVP
                     KDGPSAGVAMVTALVSLLTGRPVRPRVAMTGEISLSGIVLPVGGIKEKVLGAKRAGIR
                     EVILPSENELNVKEDLPPQILEGVQIHFVRTAEQALEIALGKFEKPTRPVTGGTRRED
                     RPLAGPVH"
     gene            complement(6231..7568)
                     /locus_tag="VN788_15145"
     CDS             complement(6231..7568)
                     /locus_tag="VN788_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HXU21917.1"
                     /translation="MNARTFAEGICGTLRATGHQAFFVGGCVRDILLGREPADYDVAT
                     DATPDRVQQLFPNSLAVGAKFGVIVVTENSGENVGLQVEVATFRSDAGYSDGRHPDHV
                     VYSKSPQEDVKRRDFTVNALLLDPETKEVLDFVGGRDDLSAGIIRAIGRPSERFSEDK
                     LRMVRAVRFAARFRYAIEGQTFGAIQKLADDIHQVSPERLRDELTKLLTEGAARRAFE
                     FLDETRLLPELLPEVARMKGVEQPPEYHPEGDVWTHTLMMIEGLPAGCPRTLAWGVLL
                     HDVGKPSTFTAPSGTGDRIRFNEHAEIGTRMAEEICRRLRFSNDDTDQITALVANHMR
                     FKDVPQMKPATLKRFVRLPRFEEHLQLHRLDCLSSHGHLDNYEFVRRFLKETPPDQVR
                     PPRLLDGNDLIGLGLKPGPEFKAILERVEEAQLDGTVTDREGATRLARSLLGL"
     gene            complement(7565..>7996)
                     /locus_tag="VN788_15150"
     CDS             complement(7565..>7996)
                     /locus_tag="VN788_15150"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HXU21918.1"
                     /translation="ELDPRTREMMCVNAGHNAPILRRSSGKIERLATGGPPFGVPLFT
                     EAPLPYPSEPVQLQPGDLLFIFTDGLVEAVNDRGEEYGEGRLLPCIEAMPTQSSDATL
                     KRVMADVNAFVGYARQHDDITCLVLCVSAQQDQAFSGVINQ"
BASE COUNT         1685 a         2492 c         2131 g         1688 t
ORIGIN      
        1 ctgctgatcg gccgacaact gctggaagcg gctcgagttc gctacttgtt tatcgaggta
       61 gctgcgccat ccaacccggt ggctaaaaag cgcgacaacg actagcgcaa gcaagcaagc
      121 caaaattatc ggcggaagcc aggtgggacg gcgcgcaatc gagtgaaacg tctcctttgg
      181 actaaagagc gccccgaaca ttctgccgac ggcgctgctg gattcgggtg tctcgacggt
      241 tacaaccgga gagctcgaca ttaaccttct cctgtgcttt caggatggag tggcttcttc
      301 tcatatttac gcgctggggg acaaaaagat tactcggcga gccgccatca gtagcggttc
      361 aatacgcgcg tcacgctcgg cgagacaagc tcgtagcgga tattgcgcca gcgaattcgg
      421 cgcgtcaaga gcgacgcaat gaagttccaa gtaaaaagga agggcacaac cggtgccaga
      481 gcaacccaca cccaactcca ttgattgagc tgatcccgcc actcgggcaa catttcgccc
      541 acagcaatcg tgcgtagcgc gcccttaatc gccgaaagca acacaactaa gagcgtcatc
      601 agcgcgagtt gcacccaagg gtcgccaccc gccatggtct ttaccaccac cacgacggca
      661 taaatcagcg ttagcgagta actcacgttc gcagccgcgc ccatcaacca gcggcgcgga
      721 gaatacaccc tcgcgatcaa catctgccga ttcgtaaatt cgagcaggct cgccggcgtc
      781 catggatgtg tggttgccgc caggcattcc gcacaaaaca aaatcggctt gccggccgct
      841 tccagagcgc gcgtcagtgc cagatcgtcg ctgacggcac ccttccatgc ttcagggaca
      901 ccttcatctt caaacgtttt ccggcggatc gcggtgccgc ctccccagca gaaattctcg
      961 cgtggccgtc ctagtaaagt ggcaatcgac ccgttccagg ccgatgccag cgcgcttgaa
     1021 aatcctccgg agccgatcga acggctaggg ataatccatc tatatgccgt ggtcgcgccc
     1081 aaccgaacgt cttgcagagg cgcaatcagc ttggccagcc agcctcgcga aaggcgcgcg
     1141 tccgagtcca cgaaaaccag aacttcgata ttctccggaa gcgattcgac ggccttcttc
     1201 agattttgta ctttgccgct gcaaccctca ggagggccgg cgaacaggat atgaacgggc
     1261 cgcacgcccg catccttcac ttcctttgca attttcgccg cgggatcgcg ctcgttggca
     1321 atcgcaaaat agatttcgta attcggataa tcgaaccgcg tcagggcggc aagattttcc
     1381 ttcaaccctg gctcgattcc cttgcatgga caaatgaccg cggccagagg cgcgtaaaag
     1441 cctgcgtgcg aacttaaccg ccctcgcacc atgcgaaacc acccgaaccc atcccatagc
     1501 gaataaagcc cgagtgcgat ctgcaccaca atcagtatgt agaagacgta aagcactgcc
     1561 gcgaggatag caaaaaccgt gaaaagagtg gcattttgcc gctcgttgac ttgctgaatc
     1621 gtcacgggta aaattcgctt tctctaataa gttgtcttct tcagaattcc ctcaccggag
     1681 gaatagatgt ctacacctgc tgctcaaccg ctaaacgaag ccaagctcca cgagttcgtg
     1741 atgaaagccg tcggagaaat gggcgcggcc atgaacgccg cactcatcct catcggcgat
     1801 aaactcggac tttataaagc aatggccggc gctggaccaa tcacatccgc cgaacttgcg
     1861 gcgaaaacca aaacggcaga gcgatacgtc cgcgaatggc tcgcttccca agcggccggc
     1921 ggttttgtcg cctacgacgc cgccaccggt aagtacaccc ttccccccga gcaggccatg
     1981 gccctcgcgg acgaacaaag ccccgttttt cttcccggct ttttcgagat cgtcagcgct
     2041 tgcatgaagg acgagccaaa gattacggaa gccttccgaa acggaaatgg cttaggctgg
     2101 cacgagcatg accattcgct cttcgcgggc acggagcgct ttttccgtcc aaattaccgc
     2161 gctcacctgg tcagcgaatg gattccggcg ctgggcgata ttgaagccaa gctaaaagcc
     2221 ggagcccgag tagcggatgt gggttgcggc ttgggaacct cgaccatcct gatggctcag
     2281 gccttcccga aatccacatt cgtcggtttt gattatcatc ccaagtctat tgagatggcg
     2341 cgcgaggccg cagccaaggc cggcgtagcg gatcgtgtca aattcgaggt cgcaaaagcg
     2401 aaagactact ccgggaaggg ttaccacctc gtcgcgttct tcgattgcct gcacgatatg
     2461 ggagacccgg aaggcgccgc ccgtcacgtg ctggaaacgc tcgaccccaa cggcgcttgg
     2521 atgatcgtcg aaccattcgc ccacgacaag ctggaagata atctgaatcc catcgggcgc
     2581 gtgtactacg gggcttcgac catgttgtgc acgcccgcct cgctatcgca ggaagtggga
     2641 ctcgggctcg gcgcgcaggc cggccaaggg cggctgacaa aggtgctcac tgcagccgga
     2701 ttcaagcgcg ttcgacgtgc cgccgaaacg ccgttcaata tcgtgctcga agcgcgcccc
     2761 tgactctcaa tgcgcgtgcc gggaaaatat ttttcgcaac actcgatcgg cggccgcatt
     2821 tagttcaagc tggaattttc cggcggcccg gcgagcctgc tccatcagcg agggatcaag
     2881 cagcagggcc accctgtcaa caaaaggacc tgatgtccgg gcgcgggcag tgcgccccga
     2941 ctggcgcctt gaatcggcgc ccgaaagttt ctcgtacagt tccaggtcat gccaaaactc
     3001 ctgggcgtcc tcgtccgtcc agtcgagcga ctgtcccagc gccacaaaaa attcctcgcg
     3061 aaaaagatcc agaaagggta ttgcgagttc gagattggct tttcttttcg tcggccgcaa
     3121 tccgccggat ttctttccgc tctttttctt tcgcggtgcc tttgcacccc gcaccgtgac
     3181 gcgtactttg agccgagccg gaagcgccgt ttcaattcgc agcacagtcg ccaaaatcga
     3241 ctcccggcga atcgtctgta actgttcgcg ataaaaagct ggctggtcgg cgcgtcccgg
     3301 ccgccggttc tcagccgcat acatatccag aaaacgagcg accatctcgt tgcgatccag
     3361 tgtcgcaagc aagatcgcag ccgcttcgcg ggcccgcacc acgagttctc ggggacgata
     3421 aggcatgaat ggttccacga ggcgcaaata cgctcgccgt ggctaccaat taggtacgcg
     3481 aatgcggcct cgctcgcaac aggaaaggcc tttcgtgcgt taaaaaacaa aaagacccgg
     3541 gcatctctgc ccgggtcttg cgcttccctc aactgaggtg gctattccgt tcccaactta
     3601 gtgtaccgga cccgcaagcg gacggtcttc ccttcgcgtg ccgccagtca ctggccgcgt
     3661 tggcttttcg aattttccca acgcaatttc gagcgcctgc tcggcggtgc gaacgaagtg
     3721 gatctggacg ccctcgagta tctgcggcgg cagatcttcc ttcacgttca gctcgttttc
     3781 cgacggcaga atcacctcgc ggatgccggc gcgcttcgct ccgagcacct tctccttgat
     3841 cccgccgacc ggtagcacga tgccggaaag tgaaatttcg cccgtcatcg ccacgcgcgg
     3901 ccgaaccggt cgtcccgtca gcaagctgac cagcgccgtc accattgcca cgccggcgga
     3961 cggtccgtcc ttcggaaccg cgccagaagg cacgtggatg tgcaggtcaa gtttccggaa
     4021 gaagtccgca tcgatgccgt aacgctcgga atttgcacgc acccagctca atgcagcgcg
     4081 catggattct tgcattactt cgcccagatg cccggtcatc gtgaactgtt ttccgccacg
     4141 catcttgctg gcctcgatga agacgatatc gcctcccacc ggcgtccaaa ccaaccccac
     4201 tgcgacgcct ggtttctgca cgcgctcttc cacttccttc tccaggcgga acttgggaat
     4261 tccaagtgcc tcccgaataa cttccggcgt aaccgtgagc ttgtccgttt tcccttcggc
     4321 tattcgcctc gcctgcttcc gaacgagcgt ggcgatttcc cgttcaagat tccggacgcc
     4381 cgcctcgcgc gtgtagtggt gaatcaggtc gtgcagcgtt tcgtcgctga actcgatctg
     4441 ttcgcccagc ttgatcccgt gctccttggt ctgccgcgga atcaaaaatc gcttggcgat
     4501 gtgaatcttt tcttcctcgg tgtagccggg gagctccaga atctccatgc ggtcgcgcag
     4561 cgcctcaggg atcgggtcca tccagttggc cgtagccacg aacagcacct tcgacaaatc
     4621 gaatggcacg tccagatagt gatcccggaa cgtcgagttt tgctccggat cgagtacttc
     4681 cattagcgcc gccgatggat ctccgcggaa atcgcggccc agtttatcta cctcgtccag
     4741 catcagcacc ggatccagcg tctccgcgcg gcgaatcccc tgaatgattt ggccaggtaa
     4801 agcgccgata tacgtccgcc ggtggccccg aatttcagct tcgtcgtgca ttccaccaag
     4861 cgatattcgg acgaacttgc gtcccatcgc tcgcgcgatc gactggccga gtgaggtctt
     4921 tcccactcca ggaggcccca cgaagcagag gattgggcct ttcatccccg gttggatttt
     4981 cttcactgcg aaataatcca gaatgcgctg tttcaccttt tcgaggtcat agtgatcctc
     5041 gtccaggatt tcctgtgcct tcgcgatgtc gatttgctct tcgcccgtgc cggaggtttt
     5101 gttccacgga agcgaggtca tccattcaag ataagtcctc gaaaccatgt actccgcgga
     5161 agcgggagtc atcttcgcca ggcgtttgag ctcgcgatcg cactcctttt tcgcttcggc
     5221 ggacatgccg gactcttccg cctttttgcg cagttcttcc acctcggact gcgcgtcatc
     5281 cgattcgccc aattctttct gaatagcttt catctgctcg cgcagcagaa attcacgctg
     5341 gctctggttc acttgctcct gcacctggtc gtgaatcttg ctccgaagtt ccagcacttc
     5401 cttttccttg gaaagctcat tgctgagcgt ttccaagcgc ttgcggacac tgaatgtctc
     5461 cagaagttcc tggcgcacca tcgtgttcag cgacggaagc gtcgaggcca caaaatccgc
     5521 gagcctcgct ggagtctcaa tgttactggc ttcggtttgc agatcgtcgg aaagctgggg
     5581 cgaacgcgcc acaacctcct tgaataattc gagtacgttg cgttcgagcg ccgtcagttc
     5641 ggcgtcccgc tcgttttcca agtctggctg cgcttccacg cgcgcccgca ggaaaggctt
     5701 caacccgacc acttcaagca cacgaatgcg ttccaaaccc tccaggaacg ctacaacgtt
     5761 gccgttgggc attcgaacca ttttgtgaac cttggccagt gtgcccacgc catgaagatc
     5821 ggtgcccgtt ggaatctcga tccgcggatc gagttgcgcc acaacgccga gaagtttttc
     5881 gggcccgtct agcgattcaa gcagggccag cgaactctcg cgcccaaccg gcagcggcat
     5941 caccgccccc ggaaacagaa cggtatctct cactggaaga atggccaact cgccgggaat
     6001 cactcttgtc gtcgtcactt tgacccccta agggctattt cgccgaggtg ggctgaaaca
     6061 cgccctcctt aaagctaatc ggctgccacc aaacttagat gcacggcaaa gcgataaaga
     6121 tttatctcat ttcttacaat attgtcagaa tagctgagca tgttacgctc ataaatgaaa
     6181 aatagcaacg ccgcgattaa agaccttaca agccgctaat taacagccac ttataggcct
     6241 aacaggctcc gcgccaatcg cgtggcccct tcgcggtcgg taacggtgcc atccagttgg
     6301 gcctcctcca cgcgctccaa aatcgcctta aattcgggac ctggcttcag tcctaggcct
     6361 atcaaatcgt tcccgtccag caggcgagga gggcgcacct ggtcgggcgg agtctccttc
     6421 aagaaacgcc gaacgaactc atagttatcc agatggccgt ggcttgaaag gcagtccaag
     6481 cggtgcagtt ggaggtgctc ttcgaatcgc ggcaggcgta caaaccgctt tagcgtagct
     6541 ggcttcatct gggggacgtc cttgaagcgc atgtgattgg ccaccagcgc tgtgatttga
     6601 tcggtgtcgt cattggaaaa ccgcagccga cggcagatct cttctgccat tcttgttccg
     6661 atttccgcgt gctcgttaaa gcgtatccgg tcacccgtcc cggacggtgc ggtaaaagtc
     6721 gagggcttcc ctacatcgtg cagtagcacc ccccaggcca gcgtgcgcgg gcagcctgcg
     6781 gggaggcctt caatcatcat cagcgtgtgc gtccacacat ctccctccgg atggtattcc
     6841 ggcggctgct ccactccttt catccgagca acttccggca acagctctgg taaaaggcgc
     6901 gtttcatcta gaaactcgaa tgcccggcgc gccgcacctt cggtgagcag cttggtaagt
     6961 tcatcgcgaa gccgctccgg agaaacttgg tgaatgtcat ccgctagctt ttggatggcg
     7021 ccaaaagtct ggccttcgat cgcgtaccga aatcgcgcgg caaagcgaac ggcgcggacc
     7081 atgcgcagct tgtcctcgct aaagcgctcg ctcggacgtc cgatggcgcg aataattccc
     7141 gcactcaagt cgtcgcggcc gccgacaaaa tcgagcactt ccttcgtctc cggatctaga
     7201 agcagcgcgt tcaccgtgaa atcgcgccgc ttcacatctt cttgcggcga ttttgaatac
     7261 acgacgtgat ccggatggcg accgtccgag taacccgcat ccgaccgaaa tgttgcgacc
     7321 tccacctgca agcccacgtt ttcaccggaa ttttccgtca ccacgatcac gccgaacttc
     7381 gcgcccactg cgagactatt tggaaaaagc tgctggacgc gatccggcgt cgcatccgtg
     7441 gccacatcgt agtccgcggg ttcacgcccc agcaaaatgt cgcgcacgca accgcccaca
     7501 aaaaatgcct gatgtcccgt cgctcgcagt gtgccgcaga tgccttccgc aaatgtgcgc
     7561 gcgttcattg gttaattacc ccggaaaacg cctgatcttg ctgtgcgctg acgcatagga
     7621 ccaaacaggt aatgtcatca tgttggcgcg catagccgac aaaggcgttt acgtctgcca
     7681 ttactctttt caaagttgcg tccgagcttt gggtcggcat ggcctcgatg catggcagga
     7741 gccgcccttc accgtattcc tctccccggt cgttgacggc ctcgactaga ccatcggtga
     7801 agatgaagag cagatcaccc ggctgaagct gcacgggctc cgacgggtag ggcaaaggtg
     7861 cttccgtgaa cagcggaacg ccgaagggcg gacctccggt tgcgaggcgc tcgatttttc
     7921 ctgaagagcg gcgcaaaatc ggagcattgt gtcccgcgtt cacgcacatc atctcgcgcg
     7981 tccgaggatc gagctc
//
LOCUS       DATNDT010000460         8256 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1367262, whole genome shotgun sequence.
ACCESSION   DATNDT010000460 DATNDT010000000
VERSION     DATNDT010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8256
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367262"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1305)
                     /gene="gpmI"
                     /locus_tag="VN788_15155"
     CDS             complement(<1..1305)
                     /gene="gpmI"
                     /locus_tag="VN788_15155"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HXU21919.1"
                     /translation="MPKRPRPIVLTVLDGWGYRPETKGNAIALARKPNYDRLLKEFPN
                     TLIHTSGPYVGLPEGQMGNSEVGHLNIGAGRIVQMDITRVDQLIASGEFFRQPLLFEA
                     MERGRSHQLHLIGLLSDGGVHSHIEHLFSLLRMARENKVSKVFVHCFMDGRDTPPESG
                     IDFLRQLEQKIREYGVGQIASISGRYYAMDRDNRWERIEKAYRAMVHGDAEAKFADPI
                     AAIRASYEKGVTDEFVVPAVITTQAAPGRSGVPRGIIHDDDAVIFFNFRADRARQITR
                     ALIEPGFTGFADPARPTNLAFVGMTQYEKTWTWLRYLLGPEKLEHILANVFAELQFKN
                     LRVAETEKYAHVTYFFNGGVEKPFAGEERVLVPSPKVPTYDLKPEMSATGVADTVIHA
                     IEKGDFDAIIMNFANADMVGHSGKLEATVKACEVVDDCLGRIF"
     gene            complement(1862..2395)
                     /locus_tag="VN788_15160"
     CDS             complement(1862..2395)
                     /locus_tag="VN788_15160"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HXU21920.1"
                     /translation="MKRYAAIMVGMIALLASPLFGQGEAPSNVQAIQQSYNTVKNNIL
                     KAADKMSDADYSFKPTPEERSFGGWVGHVADAQTAGCSRALGTPKTPSASSKTTKADL
                     VTALKESFDTCDAAYAALTEANANEPVQSFRGPLPRLAALTGNVVHDNECYGSMAVYL
                     RLKGIVPPSSERMRPAR"
     gene            complement(2559..3509)
                     /locus_tag="VN788_15165"
     CDS             complement(2559..3509)
                     /locus_tag="VN788_15165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21921.1"
                     /translation="MSSTTETLISRLQPDAQGHSVLLNHRTQSVTAIDGWKSALFGTP
                     FLAAGIFIGWIATNGSSVDKHTPNWIIAAFGVMFFLSGLFLIIHGLLGVARKAAWRRE
                     AAQYPGEPWRYDFHWHREGISFSAFDDMLKRLLSALVWTAFLVPFAWIGANEKGAWPF
                     LAAAVIFGLIGLIFWYRWAAMLFDLLRYGNSFLVYDSFPYSRGGTLRARLRSPHHVSA
                     IDELTLTLRCVQEKYVTSGSGQNRSTNVVCYELYGESSTFNRDQLTGLAGNDILMEFR
                     LPSGQPATTLAATPPTYWEIEAKGKARGADYEATFLVPVY"
     gene            complement(3608..4912)
                     /gene="eno"
                     /locus_tag="VN788_15170"
     CDS             complement(3608..4912)
                     /gene="eno"
                     /locus_tag="VN788_15170"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014264636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HXU21922.1"
                     /translation="MSTRIIAVHGREILDSRGNPTVEADVRLEGGIVGRAAVPSGAST
                     GEHEAIELRDGDKSRYLGKGVQKAVAHVNAEIAKAVGGLDASDQRTLDRRLIEADGTP
                     NKSNFGANAILAVSMAAARAAASAAGQPLYKYLAKYSSDASAAILPLPMMNILNGGAH
                     ADNSVDVQEFMVMPLGAPNFREALRWGVEVFHNLKAVLKKNGYSTAVGDEGGFAPNLK
                     SNEEALERVLEAITAAGYKPGEQIAIALDPAASEFYDRASKKYIFKKSDKSARTSAQM
                     VEFWSNWVRQYPIVSLEDGMSEEDWDGWKLLTREAGGKIQLVGDDIFVTNPEIFARGI
                     SEKIGNSILIKLNQIGSVIETVDAVEMARKANYTAVISHRSGETEDAFIADFAVAMGA
                     GQIKTGSASRTDRIAKYNQLLRIEEELGSAARFPGRSVLDRH"
     gene            5099..5344
                     /locus_tag="VN788_15175"
     CDS             5099..5344
                     /locus_tag="VN788_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015204033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HXU21923.1"
                     /translation="MLGSASINAMNYRFSALITKEDDWYVARYPELGVTSQGKDVESA
                     RASLREAIELYLETWGGPPESDNLSQQPFWTFVEIAK"
     gene            5346..5552
                     /locus_tag="VN788_15180"
     CDS             5346..5552
                     /locus_tag="VN788_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004076072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HXU21924.1"
                     /translation="MARFPVRALKRSGFVVLRQKGSHVSLEKRIGDKVVRTVVPQHST
                     LAKGTLSDILKQAELTPDDLNELL"
     gene            complement(5582..7972)
                     /gene="gyrB"
                     /locus_tag="VN788_15185"
     CDS             complement(5582..7972)
                     /gene="gyrB"
                     /locus_tag="VN788_15185"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HXU21925.1"
                     /translation="MPAAGHKYDATSIKVLGGLEAVRKRPAMYIGSTGELGLHHLVWE
                     VVDNSVDEAMAGSCDEINVLVHEDDSVTVIDNGRGIPVDMHATEKKPAAEVVMTVLHA
                     GGKFDSDTYKVSGGLHGVGVSVVNALSDWLDLEIWRDGEVWEQSYEKGIPTSKLKASG
                     KTKKTGTKITFHADPSIFTTTAYSFDTLSQRLRELAFLNKGLKITLEDERSSNSKKTE
                     FRFTGGISEFVKHLNRGKGTLHDSPIYMEGKRGQVDIEIAVQYNDSYAENIFAFANTI
                     NTVDGGTHLAGFRSALTRTINYYANSFGLLKEQKDEVSINGDDVREGLVAVVSVKLPQ
                     PQFEGQTKGKLNSDIKGLVESLVNEKLGEYFDKHSSIARKIIGKCIDAARAREAARKA
                     RELTRRKSALDSSGLPGKLADCQERDPARCELFLVEGESAGGSAKGGRDRRYQAILPL
                     KGKILNVEKARYDKMLGHEEIRAIITALGTGIGKDDFDAGKLRYHKVILMTDADVDGS
                     HIRTLLLTFFFRHMRELIEKEHVYIAQPPLYRVKRGKMDRYIRDEREFSRELMKRATE
                     EHVVKSKSGKSLEGGDLTQFLLNIQEYDQVSAKLTRRLRDAKLVELLAESELEKKTDF
                     ADKKKLQEFAKAIEKAKLDLEAKLEFDEEHSLHELLLKNGGERRINWALVASPDYKRL
                     RTLRQAIEENDKPPFTISHNGDKATKESAQQLLGYVLEDAKKEFTITRFKGLGEMNPE
                     QLWATTMNAETRTLLKVRLEDAVAAEDIFSTLMGENVEQRRKFIEDNALDVVNLDI"
BASE COUNT         1630 a         2168 c         2583 g         1875 t
ORIGIN      
        1 aaaaatccgg cccaagcaat cgtccaccac ttcgcaagct ttcacagtcg cttcgagttt
       61 gcccgagtga ccgaccatgt ccgcattggc gaaattcatg atgatggcgt cgaagtcgcc
      121 cttctcgatg gcgtgaatca ccgtatcggc gacgccggta gcggacattt ccggcttcag
      181 atcataggtg ggaactttcg gcgaaggcac gaggacgcgt tcttctccgg caaacggttt
      241 ctcgacgccg ccattgaaga aatacgtgac gtgtgcgtat ttctcggttt ccgcaacgcg
      301 caagtttttg aattgcagct ccgcaaagac gttggcgagg atgtgctcga gcttttcagg
      361 accgagaagg tagcgtagcc aggtccaggt tttttcgtac tgagtcatgc cgacgaacgc
      421 gaggtttgtc gggcgggcag ggtcggcgaa gcctgtgaat cccggctcga tgagagcgcg
      481 agtgatctgg cgggcgcggt cggcgcgaaa attaaagaaa atcaccgcgt cgtcgtcgtg
      541 gatgatgccg cgtggaacgc cggatctgcc cggcgcggct tgggtggtga tcacggcggg
      601 gacgacgaat tcatcggtca cgcctttttc gtaactcgcg cggatggcgg caatggggtc
      661 ggcgaacttt gcctcggcat cgccatgaac catggcgcgg taagcttttt caatgcgctc
      721 ccagcgatta tcgcgatcca tcgcgtaata gcgtccgctg atactcgcaa tttgtcccac
      781 gccgtattcg cgaattttct gttcgagctg gcggaggaaa tcaatgccgc tttcgggcgg
      841 ggtatcgcgc ccgtccatga agcagtgaac aaaaactttc gagactttgt tttcgcgcgc
      901 catgcgcagg agcgaaaaaa ggtgttcgat gtgagagtga actcctccgt cgctcaggag
      961 cccaatgagg tgcaattggt gggagcggcc gcgctccatc gcttcgaaga gcaagggctg
     1021 gcggaaaaat tcgccgctcg cgatgagctg atcgacgcgg gtgatatcca tttgaacgat
     1081 gcgacccgcc ccaatattca ggtgaccgac ctcgctattg cccatctggc cctcaggaag
     1141 gccgacgtag gggccggagg tgtggatcag ggtgttggga aactccttca gcagacggtc
     1201 gtaattgggc ttgcgagcca aggcgatggc gttacccttg gtctcggggc ggtaacccca
     1261 gccgtcgagg acggtgagga cgatgggcct gggtcttttg ggcatgagat cggctaaaac
     1321 gcccctccaa ttttgtgacg ggtagagctt gcaccgatcc tttgttttga atgagttacg
     1381 gcgtgttcta ctcggctgta gtgtcgatta gaatcaattt gctgtgcctt gatgacactc
     1441 ggctcgctgc ttatggctag agtgcggggg atcctggtta ctagcacagc agcggctccg
     1501 atcacgacct ggcggtgacg gccgggtaca agcccgccac gaacccagaa ccggtccgtc
     1561 gtggctaccc gggccgcctg acggccccat tcggactttg agcactcaat catctcaatt
     1621 tacgttagca cgcgggatta cggttgcaag gggttgttgg tgtaaagaat tggatttgaa
     1681 accccaggcg ggaacgcacc gcagaggtgc caaggtggca gagaactgcc gcgagagagc
     1741 cgacagaaat tttcggccgc atgcgccgga cgtttgtcgc atctccttcg accaacggga
     1801 gagaaagacc ggcgtactcc gctcggaatg actcgggcct cgagcgggag gctgctgtgc
     1861 gctaacgggc tgggcgcatg cgctcgctgg agggcgggac gatgcccttg aggcgcaaat
     1921 agacggccat ggacccgtag cactcattat cgtgcaccac gttgcccgtg agggccgcga
     1981 ggcgcggcag cggtccgcgg aagctttgta cgggctcgtt ggcattagct tcggtgagcg
     2041 cggcataggc ggcgtcgcag gtgtcaaagg attccttgag ggcggtgacg agatccgctt
     2101 tcgtggtctt cgagctggcg ctgggagttt tgggcgtgcc gagagcgcgc gagcagccgg
     2161 cggtctgcgc gtcggcaaca tggccgaccc agcctccgaa ggaacgttct tccggagtgg
     2221 gcttgaacga gtaatccgcg tcggacattt tgtccgcggc tttcagaata ttattcttga
     2281 ccgtgttgta cgactgttga attgcttgca cattgctcgg agcttcacct tggccgaaga
     2341 gcggcgaagc gagcagcgca atcattccca ccataattgc ggcatagcgt ttcattgcaa
     2401 tttccccctt tgacttcagc gaaacgaggc ccagcggatt atagacctga attggatggc
     2461 ctggaatcgg agtttgggtg cgagcggcag tgaaagagca gcggcacgcc gagccagaat
     2521 cggagcttgc gcccggctag ctcgacgata atgaaacttt agtagacggg aacgaggaag
     2581 gtggcttcgt agtctgcgcc tcgggcttta cctttggctt cgatttccca ataggtgggc
     2641 ggagtagcgg cgagagtggt ggccggctga ccggacggca ggcggaattc catgaggata
     2701 tcgttgccag cgaggccggt caattggtcg cggttaaatg tcgagctctc tccgtaaagc
     2761 tcatagcaaa caacgtttgt ggagcggttc tggccgctcc cgctggtgac gtatttttcc
     2821 tggacgcagc ggagggtgag ggtcagttcg tcgattgcgg aaacgtgatg cggagagcga
     2881 aggcgcgcgc gcagggtccc gccgcgcgaa tacggaaagg aatcgtatac gaggaagcta
     2941 ttgccataac gcagcagatc gaagagcatc gcggcccagc gataccaaaa gatcagccca
     3001 atcaaaccga aaattacagc agccgcaaga aatggccacg cacccttttc attcgcccca
     3061 atccacgcaa atgggactag aaacgcggtc cataccagag cggacagaag ccgcttgagc
     3121 atgtcgtcga acgcagagaa gctgatgcct tcgcgatgcc agtgaaaatc gtagcgccaa
     3181 ggctcgccgg gatattgcgc cgcttcgcgc cgccaggcgg ctttgcgggc gacgccaagg
     3241 agaccgtgaa taattaagaa gaggccgcta agaaaaaaca taacgccaaa cgcagcgatg
     3301 atccaattcg gagtgtgctt gtccaccgag ctgccattag tcgcgatcca cccgatgaag
     3361 attccggccg ccaggaacgg cgtgccgaac aacgcggact tccagccgtc aatggccgtg
     3421 acgctctgcg tgcggtggtt gaggagcact gagtgaccct gggcgtcggg ctggagccgt
     3481 gaaatcagcg tttcagtggt ggaagacatg acagccgttt tacctcgtat acgaagcttg
     3541 acaagatgaa tcgcgagcaa caatgggact tgcgcgaaaa agtaaagagc gggtgcaatt
     3601 cgatgcgcta gtgccggtcg aggacggagc ggccggggaa gcgagcggcg gatcctagtt
     3661 cttcctcgat gcgcaggagc tggttgtatt tggcgatgcg gtcggtgcgg gaggcggagc
     3721 cggtcttgat ctggccggcg cccatggcta cagcgaaatc ggcgatgaag gcgtcttcag
     3781 tttcaccaga gcggtgcgag atgaccgccg tgtagttcgc tttgcgggcc atttcgacgg
     3841 cgtcgactgt ttcgatgacg ctgccaatct ggttcagctt gatgaggatc gaattgccaa
     3901 ttttttcgga gatgccgcgc gcgaagattt cgggattggt gacgaagata tcgtcgccca
     3961 caagttgaat tttgccgcct gcttcgcgcg tgaggagttt ccagccatcc caatcctcct
     4021 cggacatgcc gtcttcgaga gacacgatgg gatactggcg gacccaattg ctccagaatt
     4081 cgaccatctg cgcggaggtg cgggcagatt tgtcggattt cttgaagatg tattttttgc
     4141 tggcgcgatc gtaaaactcg ctggcggcgg ggtcgagggc gatggcgatt tgctcgccgg
     4201 gcttgtaacc agcggcggtg atggcttcga ggacgcgctc gagagcttct tcgttggatt
     4261 tcagattggg cgcgaagccg ccttcgtctc ccacggccgt ggaatagccg tttttcttca
     4321 gcacggcttt caaattgtgg aagacttcga cgccccagcg gagcgcttcg cggaaattcg
     4381 gcgcgccgag cggcatgacc atgaattcct gcacgtccac ggaattatcc gcgtgcgcgc
     4441 cgccattgag gatgttcatc atggggagag gaagaatcgc ggcggaagcg tcgctcgaat
     4501 atttcgcgag gtatttgtag agaggctggc cggcggcgga tgctgcggca cgcgcggcgg
     4561 ccatcgagac ggcgaggatc gcattcgcgc cgaagttgga cttgttggga gtgccgtccg
     4621 cttcgatcag gcggcggtca agcgtgcgct gatcggaagc gtcgaggccg ccgacggcct
     4681 tggcgatttc ggcattcaca tgcgcgactg ctttctgcac gcccttaccg agatagcgcg
     4741 atttgtcgcc gtcgcgaagc tcgatagcct cgtgttcgcc ggtggatgca ccggaaggga
     4801 ccgcagcgcg gccaacgatg ccgccttcta agcgaacgtc agcttcgacg gtgggattgc
     4861 cgcgcgaatc aagaatttcg cggccatgaa cggcgataat tctggtggac attttcctct
     4921 ccgtgaaaac cgcgattatc gcacgagttg gcccggagcc atggcttcta tagtgtcagc
     4981 ttataggatc aatgcggtgg gctctaacgg gcagcgatat cgccggtgtg aatctcgaag
     5041 tccgagagtt gatcggccat gttttctccc taaacccgag tcacaggctt gcgcgcatgt
     5101 gctcggttcc gctagcatca atgccatgaa ctaccgcttc tcggccctga ttaccaaaga
     5161 agacgattgg tacgtggccc gctatccgga actcggtgtg acgtcgcaag gcaaggacgt
     5221 tgaatcggcc cgggcgagtt tgcgggaagc catcgagctt tatcttgaga cctggggcgg
     5281 gcccccggaa tcagataact tatcgcagca acctttttgg acgttcgtcg agatcgcaaa
     5341 gtgacgtggc gcggttcccc gttcgggcgc tcaaacgcag cgggttcgta gtcctgcgac
     5401 aaaagggcag ccacgtttcg ctcgaaaaaa gaatcggcga caaggttgtc cgcacagttg
     5461 tgccgcaaca ctcgactctc gcaaaaggaa ctctttccga tattctcaag caagcggagc
     5521 tgacaccgga cgacttgaac gagttgttgt agtctcgcct cgcattgtta ttccttcgcg
     5581 tttaaatatc gagattgacg acatcgaggg cgttgtcctc gatgaatttg cggcgctgtt
     5641 ccacattttc gcccattagc gtggagaaaa tatcctcggc ggcgacggca tcttcgaggc
     5701 gcactttcag aagggtgcgc gtttcggcat tcatggtggt ggcccagagt tgctctggat
     5761 tcatttcacc gaggcccttg aagcgcgtga tggtaaattc tttttttgca tcttcgagaa
     5821 cgtagcccag gagctgctga gcgctttctt tggtggcttt gtcgccattg tggctgatgg
     5881 tgaagggcgg cttatcgttt tcctcgatgg cctggcgcag ggtgcgcagg cgtttgtagt
     5941 cgggtgaagc gacgagggcc caattgatgc ggcgttcccc gccatttttt agaagcagct
     6001 cgtggaggct gtgctcttca tcgaattcga gcttggcttc aagatcgagt ttcgcttttt
     6061 cgatggcttt cgcaaattct tggagcttct ttttatccgc aaagtcggtt ttcttttcga
     6121 gctcgctttc cgcaaggagc tcgacgagct ttgcgtcgcg caggcggcgt gtgagttttg
     6181 cggagacctg gtcgtactcc tgaatattca gaagaaactg ggtaaggtcg ccgccttcga
     6241 gcgacttgcc gctcttggat ttgacgacgt gctcttcggt ggcacgcttc atcagctcgc
     6301 gggaaaattc gcgttcatcg cgaatgtagc ggtccatttt gccgcgcttg acgcgataga
     6361 gcggcggctg tgcgatgtaa acgtgctctt tttcgatcag ctcgcgcatg tggcggaaga
     6421 aaaaagtgag cagaagagtg cggatatggc tgccgtcgac atccgcatcg gtcatcagaa
     6481 tcactttgtg atagcgaagc tttccggcgt cgaaatcatc tttgccgata cccgtgccca
     6541 gcgcggtaat gatggcgcga atttcctcgt ggccgagcat tttgtcgtag cgtgcctttt
     6601 cgacgttgag aattttgccc ttcagcggaa ggatggcctg atagcggcgg tcgcgaccgc
     6661 ctttggccga gccgccggcg gattcgccct caaccaggaa gagttcgcag cgcgcgggat
     6721 cgcgttcctg gcaatcggcg agctttccgg ggaggccgct tgaatcgagc gcggatttgc
     6781 ggcgcgtaag ttcgcgggct ttgcgggctg cctcgcgcgc gcgcgcggcg tcaatgcact
     6841 tgccgatgat ttttcgcgcg atcgatgaat gcttgtcaaa atattcgccg agcttttcgt
     6901 tgaccaacga ttcgaccagg cccttgatgt cgctattgag cttgcccttg gtctggccct
     6961 cgaactgcgg ctgagggagc ttcacggaaa cgacggcgac gagcccctcg cggacgtcgt
     7021 caccgttgat ggagacttca tccttctgct ctttgagcag gccgaaggaa ttggcgtagt
     7081 agttgatggt gcgcgtgagc gcggaacgga agccggcgag atgcgttcct ccatcgacgg
     7141 tgttaatcgt gttcgcgaag gcgaaaatat tttcggcgta gctatcgttg tactgcacgg
     7201 cgatctcgat atcgacttgc ccccgcttgc cttccatgta gatgggcgaa tcgtgtaggg
     7261 tgcccttgcc gcgattcaga tgcttgacga attcggagat gccgccagtg aagcggaact
     7321 cggttttttt actgttagag gaacgctcgt cctcgagagt gattttgagg cccttgttga
     7381 gaaacgcgag ctcgcggaga cgctgcgaaa gcgtgtcaaa gctataggcg gtggtggtga
     7441 aaatcgatgg gtcggcgtga aaagtgattt tcgtgccggt ttttttggtt ttgccggaag
     7501 ctttcaattt tgaagtggga atgccttttt cgtaggactg ttcccagact tcaccgtcgc
     7561 gccagatttc gagatcgagc cagtctgaga gcgcgttgac gacggagacg ccgacgccgt
     7621 gaaggcctcc tgaaactttg taagtgtcac tatcgaattt tccgcccgcg tggagcacgg
     7681 tcatgaccac ttccgcggcg ggcttttttt ccgttgcgtg catatcgacg ggaatgccgc
     7741 ggccattgtc gattacggtg acgctgtcgt cttcatgcac gagcacattg atttcgtcgc
     7801 aggatccggc catggcctcg tcaaccgagt tatcgacgac ttcccagacc aagtgatgca
     7861 ggcccaactc gccggttgaa ccaatgtaca tcgccggacg cttgcgaacg gcttcaagtc
     7921 caccgaggac tttgatcgaa gtcgcgtcat acttgtgccc agcggccggc atttcttttt
     7981 cagccctgaa gtacgtatct cctcaggtgt tgcgtgctgc ggtggtgctg ggtcgtgcgt
     8041 gggaaggcgg tcgcgcggat gagccggaga cttgccttgc ccattggtcc ccgctgcgag
     8101 aaatttttcg cttgagccgg gactcgaagg gtatttttag agcttcatcc cgtccgattc
     8161 cgaatttttc gggcggacgc ggcgaaactc cattccccaa ttaggggatt tatttcaaac
     8221 caatttgcgc gacgatcgga ctcgctgcgt ctggcg
//
LOCUS       DATNDT010000461        25597 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3270234, whole genome shotgun sequence.
ACCESSION   DATNDT010000461 DATNDT010000000
VERSION     DATNDT010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 25597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25597
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3270234"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            356..745
                     /locus_tag="VN788_15190"
     CDS             356..745
                     /locus_tag="VN788_15190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HXU21926.1"
                     /translation="MTLTRDCKETIQARVQRDRGFRQALLREGIENFLSGDVETGKII
                     LRDFINATVGFPKLSEITHRSAKSLMRMLGPRGNPQARNLFEIVKYLQETEGVQFEVR
                     PRRAASRTKRTGGPAAVLVGGRRSSLK"
     gene            1198..3174
                     /locus_tag="VN788_15195"
     CDS             1198..3174
                     /locus_tag="VN788_15195"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HXU21927.1"
                     /translation="MEYRRYRDEGKNALSASRYTEAIEAFGHAALFHPNDPEAQFNLG
                     KAFQSAGKIEAARQAYIRALDVNPGFSEVRRVLLSLPPLPPGRSEFPINQLVHAPDLE
                     LAFWVLEVRKGGFGAVYVVEEWPGGLPPFDERGKFVDEERSALKTLQARFLWSDEDRD
                     RFERECLHWIMLDRHPNIVHARAVVKIEGFPCLWLEYCAHNLGDVLRAGPLSLDTALR
                     LSFQFCDGMLHAHHKMGLVHRDIKPSNCLLSNDNRTLKISDWGLSRVFRSTGDKTFGL
                     LGLSRETESQLTCAAGTPQYMASEQFQPAARLDTRTDIYSFGIMLYEMLTRDLPPVGY
                     MAYSHIACTAVAEQIPSRLRQIILRCVHPNPDERPRDFREVRSLLETTYSQKTTSAAP
                     AEAKPLEMSVGDWNDKGLTLSRMGYPAEACECYEHALNISPSEPTVLVNYSGELCSLG
                     RLDDALLRIDQGLNIDPENVGLWKNKSIVLEKLGRVEESDACFERLLDLQAKWPSGYY
                     QWRDMTVGCYKRRKYELALKCCDAALGINKRDADVWKYKAIILHTMGRYEAALVSCEE
                     GLAIEPREHELWNIRAVTLTALERFEEALLACNRGLEIDGGYILLWQNKATALRSLDR
                     PVEAEKCSEMAEELEASRRLADSTETRVNGGEDR"
     gene            3339..3917
                     /locus_tag="VN788_15200"
     CDS             3339..3917
                     /locus_tag="VN788_15200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21928.1"
                     /translation="MGLWNAAVHFWKALSNADKINFAIAVCTAFAALGAAYSALLARR
                     SIRGAVEQFRAQAFLGVLAYEREVNFSVQMDVIRSLCGKPAKDLTEAEQKSILVVVNF
                     LNHVAHLIRHCYVVPKQMLLLYAPSIAACRDNLLQDGQWLKELRKQTGEARYYLHFAK
                     LCQSETEDLIWKDMAAKIVWTTDPYEPQLPPV"
     gene            4187..4756
                     /locus_tag="VN788_15205"
     CDS             4187..4756
                     /locus_tag="VN788_15205"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU21929.1"
                     /translation="MDRRNFLQMSALAATALPIQGAFAMQTASQQTRGAATIISPKGA
                     QAYSMMGGGEARLLVTGEKTKGAWWMGRFREDPGFTTQLHYHPKADEQFYVLEGVLSV
                     YVVDAWHELGPGTLGLLPHGTPHAQANHSDKPVHFLGSGAPAGFEKFFPAVDGLMKRR
                     LKPGTPEFAAEIRKIAAGCDMVPLGPPPK"
     gene            complement(5321..5530)
                     /locus_tag="VN788_15210"
     CDS             complement(5321..5530)
                     /locus_tag="VN788_15210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21930.1"
                     /translation="MLIKDLNFIVLNQGQFTRPADRSIHGWVRIDCPNEADRNDAAAW
                     SKTVCQELPEPDVAPQSVVIGLEKS"
     gene            complement(5787..6017)
                     /locus_tag="VN788_15215"
     CDS             complement(5787..6017)
                     /locus_tag="VN788_15215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21931.1"
                     /translation="MRVYWERIGVLGPICRLVGKGFNDREIADKLNITEITVQGCVSW
                     VLHSFKMPGRMELARDAFSAERSAHSAHLKGR"
     gene            complement(6103..7734)
                     /gene="groL"
                     /locus_tag="VN788_15220"
     CDS             complement(6103..7734)
                     /gene="groL"
                     /locus_tag="VN788_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521902.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HXU21932.1"
                     /translation="MAKQIVTGVNSRQAILRGVNILADAVKITLGPKGRNAVIEKKFG
                     GPIITKDGVTVAKEIELPDPLENMGAQMVKEVASKTSDVAGDGTTTATVLAQAIFREG
                     VRTLAAGANPMALKRGIEKAVEMAVGEIKRLHKDVKGDMIAQVGAISANNDRQIGSII
                     AEAMRKVGKDGVITVEESKTMETTLEVVEGMQFDRGYLSPYFVTAPERMECVLEDVFI
                     LIHEKKISSLRELLPLLEQTTKMSKPLLIISEDVEGEALATLVINKLRGTLQCAAVKA
                     PGFGDRRRAMLEDVAVLTGGKAISEDLGVNLENVKVEELGRAKKVIIDKDNTTIIEGG
                     GKASEIEGRIKQLRTQIEDSNSDYDQDKLQERLAKLVGGVAVIKVGAATETELKEKKA
                     RVEDAMHATRAAVEEGIVPGGGVALLRCIPALAKLNLQDDEATGVNIVRRALEEPTRQ
                     IAQNAGREGAIIVGRVRDSKEEDFGFNAETGEFGDMIKAGVIDPAKVTRLALQNAASI
                     VALMLTTEVLIADIKQEEEMTAVGAGQSGGTGGMY"
     gene            complement(7798..8115)
                     /locus_tag="VN788_15225"
     CDS             complement(7798..8115)
                     /locus_tag="VN788_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HXU21933.1"
                     /translation="MNVTPLHDRVLVRRLEEKETAKGGIIIPDTAKEKPHEGEVIAVG
                     AGKLNGKGGRIPLDVRRGDRILFGKYTGNDIKIDDKEYLILREEDILAKVSGIAKAAS
                     SKK"
     gene            complement(8464..8979)
                     /locus_tag="VN788_15230"
     CDS             complement(8464..8979)
                     /locus_tag="VN788_15230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21934.1"
                     /translation="MAMRKDQLRDAIRDGAVLIKDGTLLPESLRIENEPCVPGWGLVQ
                     SLNGYAFDRKVRETGWTFFCLAGEIGTIVLGTDEQFMLRRAIKRILANSRSSPFNSLE
                     ITRVARRHFLGLPYLSVGAQSRHVQESMVLLSAERTRESTKTKSTVTPARAGSRVRKD
                     LIPKERAGMSA"
     gene            complement(8989..9699)
                     /locus_tag="VN788_15235"
     CDS             complement(8989..9699)
                     /locus_tag="VN788_15235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HXU21935.1"
                     /translation="MIRKSVLVVCLGLLAAAPVFAANKEQKRLHNAGVVMQEIMNTPE
                     NIPQEVIEKAECVIVFPSVLKAAFGIGASYGRGAMVCRTGKDFRGAWGAPAMMALEGG
                     SVGFQIGGQATDLVLLIMNKRGASSILSSKVKLGADASVAAGPVGRDAAANTDAHMRA
                     EVLSYSRSRGLFAGISLEGSTLRPDDDATAGVYGRKLTAREIVLGGEARVPASGRLLV
                     SVLQKTSPRNESKESAGN"
     gene            complement(10161..10364)
                     /locus_tag="VN788_15240"
     CDS             complement(10161..10364)
                     /locus_tag="VN788_15240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21936.1"
                     /translation="MDESKRQAVQFLEKEIKTYIALALFLSKAGIKERVQAGDKEVLI
                     SPAYYKDRMKEARKLVNELRKPN"
     gene            complement(10466..10621)
                     /locus_tag="VN788_15245"
     CDS             complement(10466..10621)
                     /locus_tag="VN788_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018423731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HXU21937.1"
                     /translation="MLGTILIVSLVMALLAVLPRWSHSRKWGYGPTGGVSLLLLVIII
                     FVLLGVI"
     gene            complement(10686..11348)
                     /locus_tag="VN788_15250"
     CDS             complement(10686..11348)
                     /locus_tag="VN788_15250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21938.1"
                     /translation="MKTIRVIGASVLLLLLGAAAPTYARQDQHAKPPKREQKAKPERQ
                     RQAEPARQGQQAKPERQQQAEGKQEQQQRQQQTKSTRQEQQQEAKSRQERTKSHNQQQ
                     VKQVTATRQDQEQHARGQHGQQPEHAQGKWARNGQKDSRGHEYTESRFGPQHHARFEE
                     HGGRYHNGRREYSDGGYWFYAGAYPAWFYQQDVYFTMGTDGLWYAVAYSDPSLIFQVY
                     IE"
     gene            complement(11471..11662)
                     /locus_tag="VN788_15255"
     CDS             complement(11471..11662)
                     /locus_tag="VN788_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020680884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Thivi_2564 family membrane protein"
                     /protein_id="HXU21939.1"
                     /translation="MPILQILMVLIVVGVLLWLVNRYVPMQGTIKSILNAVVVICVVL
                     WLLNIFGLFHSLSHIRVGT"
     gene            11959..12642
                     /locus_tag="VN788_15260"
     CDS             11959..12642
                     /locus_tag="VN788_15260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011502162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HXU21940.1"
                     /translation="MAVTTGDPNRPKANRKHAFNAQDFLDSAGIARRVVEFKGKQAIF
                     SQGDRANNVLYIQKGGVRLSVVNETGKEAVVAIFGPGDFFGEGCLAGQPIRMATATAV
                     MLTTALVIEKNEMVRVLHAEHAFSDRFISYILSRNIRVEEDLVDQLFNSSEKRLARML
                     LLLARYGKQDKPQRVLPKISQQMLAEMVGTTRSRVNFFMNKFRKLGFIKYNGGLQINS
                     SLLSVVLHD"
     gene            13822..15000
                     /locus_tag="VN788_15265"
     CDS             13822..15000
                     /locus_tag="VN788_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HXU21941.1"
                     /translation="MAEVLNELCNFIDAKAPGVIPTVLLPDGDGRLRLAAGPKVPKIW
                     NEAFDGMALHSYVSFLRAAGRGEGPVPVADITSDPSFAACWDLAVNQGVRAAWTVPIL
                     SKERKILGTLILFYPTPQPPGQRDLELMERVIHMAAIAIECHRSEEELREFSRRLYQS
                     QDEERRRIARELHDSTGQKLAILEIALSRLNRVVNSRPRESEEILLECASLTRNISDE
                     LRTLSYLLHPPMLDEFGLNSAIRCYVNGINQRKGLHVEAEISQELPRLSEEAELAVFR
                     IVQASLTNVHLHSGVALAKVKIEHSPDGIVVVISDEGRGIPDGVLDHSSQPKTVGIGI
                     TGMRERVRQLGGCMQIESSASGTSVKVTVPSRHFRTAISAADKRTFRCAADNRELHSN
                     "
     gene            15560..16264
                     /locus_tag="VN788_15270"
     CDS             15560..16264
                     /locus_tag="VN788_15270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018247522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU21942.1"
                     /translation="MNALSRILIIEDDKDIVELVRYNLEKEGFQVLACGDGASGLAQI
                     KKSPPDLLVLDLMLPKLSGLEICKEIRRDNRLNRLPILMLTARGDEADRVVGLELGAD
                     DYVTKPFSPRELVARVKALLRRLEWPSDEARAVIVGLLRIDPVSYRAESEGRQLPLST
                     LEFRLLYYLASRPNRVFSRDQLLDAVWGSERFVTPRSVDVYVRRLREKVEKNPEEPEH
                     LKTVRGAGYIFETKVN"
     gene            16277..18064
                     /locus_tag="VN788_15275"
     CDS             16277..18064
                     /locus_tag="VN788_15275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21943.1"
                     /translation="MARSGVFWKLGLTYFVLLIAVLAAVDFYAGRILRQNYIHAADER
                     LNSLALIARSNPPKFDDETALASWAAGMSRSGARVTIIAANGRVLADSAHDIETMENH
                     SNRPEFRQAMSSGEGESVRHSRTLDRDLVYWAVRFERPSGSPVVIRFATPLAEINSAL
                     AEVRRRLWEVSLVILLLAIGISVLFSRRFAKRVGELQEFSERVATGDFRPLIQESATD
                     ELGDLARALNDTASRLSATISSLSDERNRTAAILRSMAEGIAVINADEQITFYNEAFA
                     RLWNLDNRSHANRSAVSLIRHPDVLQLIRSALAGQEARGELSLGSAQPRHFSVTAATI
                     LASSDGSADSEKQSKPAGAVVVLHEITEIRRLEQVRKDFVANVSHELRTPLTAIQGFA
                     ETLLNGALDDPQNNRHFIEVIRNHASRLARLTEDLLELSKIEAGKLAMDLRSVAVADL
                     IAPVIDLAQDLALRKPVSLNVINPAIQFPMVRGDVGLLREVLRNLLDNAIQYTPAGGR
                     VEVSAERNGSSVVFTVADTGIGIPQAEHNRIFERFYRVDAARSREAGGTGLGLAIAKH
                     IVEAHGGQIWLDSEIGRGSKFHFSIPLAG"
     gene            18169..18864
                     /gene="phoU"
                     /locus_tag="VN788_15280"
     CDS             18169..18864
                     /gene="phoU"
                     /locus_tag="VN788_15280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXU21944.1"
                     /translation="MATSARSSSDFYTTALENYLVAMARTVEGSINRSFEALLSRNAE
                     IASAVFVTEPKINESEILIDEQAVRLLQRGDLSQGDVRHIVATLKINNDLERMGDLAV
                     NTCQCVIALCQSPSADTPPELASMVPPVRAMVGRCLGALTHRNVTLAFEVLQNEEAID
                     RQRDRVVEMLISAVKKEPSLATVNIQYILAARHMERIADHCTNIAEDVIFWLQGLDIR
                     HNRNSPSNPPSVE"
     gene            18987..20531
                     /locus_tag="VN788_15285"
     CDS             18987..20531
                     /locus_tag="VN788_15285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21945.1"
                     /translation="MRRRLSALIVAAIFPLVAASAQTKTNNDANSSAKENSSSAAVRS
                     NTKTATAAKLSTAEKSVTRYAISPVSAKSSDAANAAAQDTDENQPPLSFKVGGADFTP
                     VGFLDFTTVYRSTNVGSGIGTSFGSIPFNGSSPAGRLSETRFSAQNSRIGLRVDANPS
                     ANLKLTGYLEADFLGNAPGNLEVTSHSDTLRMRLYWVDAHYGKWEFLGGQSWSMMTPN
                     RKGISPMPSDIFYTQDMDTNYQAGLVWLRTPGFRVVYHADDRWTLGLALDDPEQFTGG
                     LVILPAGFNAAQVDDSGGNAKTPNAAPDVIAQIAYDTSLGSHAFHIEAAGLYRAFRLN
                     TAATNANVTRSGGGGSVNVNFEIVKNLRLMGTSFWSDGGGRYIFGLGPDFVVKTDAAG
                     NFIPSPLHAGAGIGGFEYQLIPQTMIYGYYGASYFGRDFQEVAAGSPPPFVGYGFAGS
                     NSSQNRTIQEGTVGLIETLEKSQLRRFEDHHPSFLPFTQSMVECRYSCWGKERASRHG
                     FCGPAV"
     gene            20619..21674
                     /gene="pstS"
                     /locus_tag="VN788_15290"
     CDS             20619..21674
                     /gene="pstS"
                     /locus_tag="VN788_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HXU21946.1"
                     /translation="MTKLAGQSWLVLCTLLIGVSLVSCSHNVGTTTGSQTVLINGAGS
                     TFGYPIYSRWADDFHRMHPNVEVNYQSVGSGAGIRQLLAGTVDFGASDAPMTDAQLAQ
                     AKSPILHFPTVLGAVVPTYNIPGVTAELKFTPEALAGIFLGKVTRWNDPAIARVNTGL
                     KLPSGNIVVIHRSDGSGTSFIWTDYLSKVSAEWKAKVGAGTSVNWPVGLGGKGSEGVS
                     GLIQQTPDSIGYVELTYAIQNHILYGSVQNSSGAFVKASLDSVTAAAASAADSMPKDF
                     RVSITNATGKDAYPVASFTWLLVPAQISDPTKRDAIKSFLHWMLTKGQNDAEPLAYAK
                     LPADVSTKEDATISEIR"
     gene            21677..22663
                     /gene="pstC"
                     /locus_tag="VN788_15295"
     CDS             21677..22663
                     /gene="pstC"
                     /locus_tag="VN788_15295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HXU21947.1"
                     /translation="MPAPVNVFDLTPSSRPLRVFWDRLRRGDEIAHVMTLISASTVVG
                     VTALLVCQMWIQSALSRQKFGWAFFATRVWDPVAGQFGALPFLYGTVVTSAIALLLAV
                     PLGLGTAIFLSEFSVRRISSVLAFLVDLLAAVPSVIYGLLGIFVLAPWLRVSVEPMLR
                     SHLGFLPLFRGPAYGVGFLAAGLILAVMVVPFIVSVSREVLLSVPREQREAALALGAT
                     RWETISKAVVPFAKTGIIGSVFLALARALGETMAVTMVIGNDPKISASLFAPGYSIAA
                     VIANEFTEATGSLYLSALIELGLVLFLLTFVLNGFARLLVLATAQRGAGVQQ"
     gene            22660..23508
                     /gene="pstA"
                     /locus_tag="VN788_15300"
     CDS             22660..23508
                     /gene="pstA"
                     /locus_tag="VN788_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HXU21948.1"
                     /translation="MSLRSGWRKCLNASMLGLTAVFAVATVSVLFFILGYLVYHGGKN
                     VSWNFFTRLPKPVGEPGGGMANAIVGTAKLLFVALLIGAPVGVLSGVYLSEFGGKTLS
                     FFVRYTADLLNGVPSIVIGIFAYAIIVVPARHFSTFAGGFALGIMMIPIILRSTEEFL
                     KAVPSTLREGGMALGVSKWRTVLTLVVPAASSGIIAAVMLAVARIAGETAPLLFTAFS
                     NRYWSPGFNEPVASLPVMIYTYAIGPYPEWHEQAWTAGLVLLGLVVVLNVATRLIFAR
                     KMAALR"
     gene            23598..24362
                     /gene="pstB"
                     /locus_tag="VN788_15305"
     CDS             23598..24362
                     /gene="pstB"
                     /locus_tag="VN788_15305"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017615596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HXU21949.1"
                     /translation="MDLATGRLNPRFTISGLKVSYGANEVLHGIDLKVFANVVTAVIG
                     PSGCGKSTLLRSLNRMTEVVRRAHIEGKILLDDEEIARMDVVKLRRRVGMVFQRPNPF
                     PKSIFENVAYGLRINGYKGGLSDAVEHSLRRAALWDEVKDQLQKSAYALSGGQQQRLC
                     IARALAVDPEVLLLDEPCSALDPITTAKIEELLFQLKASCTIIIVTHNMQQAARASDF
                     TAFLLEGQLVEYQSTSKLFTTPSDPRTEAYITGRFG"
     gene            24394..25059
                     /gene="phoU"
                     /locus_tag="VN788_15310"
     CDS             24394..25059
                     /gene="phoU"
                     /locus_tag="VN788_15310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXU21950.1"
                     /translation="MERHFEHDLGQLKERLLVMAGLAERAVHQSVEAVFEKSEHLANR
                     VLEEEPAINKMQIEIDDRVMQLVALQAPMAVDLRFVLCASRINNDLERIGDQAVNIAQ
                     SALRILRHPQVKPYVDLPRMSELAEGMVRESLNALVHGDAELAKSVLIRDDEVDHLRD
                     QIFRELLAYMMENSAVVFPAFELVLITKNLERIGDHATNIAEDVIYMVAGRDVRHLAA
                     DRR"
BASE COUNT         5597 a         7008 c         6988 g         6004 t
ORIGIN      
        1 gttcgatccc caccgcccct accaatcatt tctttgaacg gtaggaactt attaaaagca
       61 cgcgggggca aaaaggggta gatccagtcg atgttccggt tcgtacgcta atttctgcgg
      121 cacgagtgac aaccggattg aaggagcact cagtcggcgt tgggcggcga cgtgcgaatt
      181 gtcgccgaat ttcccgaccg tgcgccggtc gtgctttcgg gcctatccga ggacgacacg
      241 ccgcgcaaat catctcggaa acatgcgcac gccgaagtct aaggctccgc tgatcccggt
      301 gccgtcaaaa ggccgggccc gctagcacga ttcctggacc ttggagaaat gagcaatgac
      361 gttgacacga gattgtaagg aaacgatcca ggcgcgggtt caacgcgatc gaggttttcg
      421 tcaggcgctt ctgcgcgagg ggattgaaaa ctttctctcc ggtgatgtgg aaaccgggaa
      481 gatcattctc cgtgatttca tcaatgcaac ggtgggcttt cccaaactga gcgaaatcac
      541 ccatcgctcc gcaaaaagtt taatgcggat gctcgggccg cgcggtaatc ctcaagcacg
      601 gaatctgttt gagatcgtca agtacttgca ggaaaccgag ggcgtgcagt ttgaagtccg
      661 gcctaggcgc gctgcctcac gcacgaagcg caccgggggg ccggcggcag ttcttgtggg
      721 gggccggcgt tcgagcttga aatgagttgc cagcagggga atttcccgct gcctgaggct
      781 cggatacaac cgatgtgtat cagtacgaca ctcacagggg tcagttgtat agaactaaca
      841 aattgtgcga ctcacacttg gaattgggaa acctctcttt agcccctcac gacgcagatc
      901 gatggacgcc aattacgata atctgagcgc atttgtacga aggcatggat cgatcaccat
      961 cctagacaca cgaaaccggg caagaaaatt gccaacaggt gcgctcgacc tgctagatgt
     1021 catcgataaa gctgatcgct tcgtgtttga aggcaaatgg tattccagag aagaattcga
     1081 atctctcatg gatagagacc aagagcaatg aatgcgctca agtggctgag aaactggtgg
     1141 ccgcccggaa gactcccggg caggaacgga gagagcacac cacagcagag ctatgagatg
     1201 gaatatcgaa ggtaccgtga tgaaggcaag aacgcgctgt cggcatcacg gtacacggaa
     1261 gcaattgagg cgtttggtca tgccgcactg ttccatccga atgacccgga agcgcagttc
     1321 aatctcggaa aagcttttca gagtgcggga aagatcgaag ccgcccgcca agcgtatatt
     1381 cgggctttgg atgtcaatcc aggattttcg gaagtccgcc gcgttctatt gtcattgccg
     1441 ccccttcctc cggggcggag tgaattccct atcaaccaac tagtgcatgc accagatctt
     1501 gaacttgcct tctgggtatt agaagtcagg aaaggaggct tcggggcagt ctacgttgtg
     1561 gaagagtggc ccggaggtct accccctttc gatgagcgcg gaaagttcgt ggacgaagag
     1621 cgttcggcat tgaagactct tcaggcacga tttctttgga gcgatgaaga tcgcgatcgg
     1681 ttcgagcgcg agtgtctgca ttggatcatg cttgaccgcc atccgaatat tgttcatgcg
     1741 agagctgtgg tgaagataga gggtttccca tgcctttggc ttgagtattg cgcccataac
     1801 cttggagatg ttctgcgtgc cgggccactg tccctcgaca ccgctctgcg cttatctttt
     1861 cagttttgcg acggtatgct tcatgcgcac cacaaaatgg ggcttgtaca tcgcgacatc
     1921 aagccgtcca actgtctgct gtcaaatgat aatcggactt tgaaaatctc agactgggga
     1981 ttgtctcgcg tgttcagaag cactggagac aaaacattcg gtttgctcgg gctgagccgc
     2041 gaaactgagt cacagctcac ctgtgcggcc ggtactccac aatatatggc ctcggaacaa
     2101 tttcaaccag cggcgcgctt agacacgaga acggatatct actccttcgg aatcatgctt
     2161 tatgaaatgc tgacacggga cctcccgccg gtcggctaca tggcgtattc ccacatcgcc
     2221 tgcacggccg tcgcagaaca gattccaagc cgcctgcgac aaatcatctt gcgatgtgta
     2281 catcccaatc cagacgagcg cccgagagat tttcgcgagg taagaagcct gcttgagacc
     2341 acctattcgc aaaagactac gagcgcggcg cctgccgagg cgaaaccact ggagatgagc
     2401 gtcggcgatt ggaacgacaa agggctgacg ttgtcgcgga tgggatatcc cgccgaagct
     2461 tgcgagtgtt acgaacatgc tctgaacatc tccccgagtg agccgactgt cttggtaaat
     2521 tacagtgggg aactgtgcag cttaggtcgg ctcgacgacg cacttctgcg aattgaccag
     2581 ggcctgaaca tcgaccctga gaatgttggc ctgtggaaga acaaatccat cgtcctggag
     2641 aagctcggac gcgttgagga atcagacgct tgcttcgagc gattgttgga cctgcaagca
     2701 aaatggccat ccggttacta ccaatggcgc gatatgacgg ttggttgcta taagcgacgg
     2761 aaatacgagt tagccctgaa gtgctgcgat gccgcccttg ggatcaacaa acgtgatgcc
     2821 gacgtatgga agtacaaagc gatcatcctc cacacgatgg gccgttatga agcggcgttg
     2881 gtcagctgcg aggaaggact tgccatcgag ccgcgagaac atgagctgtg gaatattcga
     2941 gctgtaactc tcaccgccct tgagcgtttc gaggaagcat tgttagcctg caatcgagga
     3001 ctcgagatcg acggagggta catcctgctc tggcagaaca aagcaactgc tctccgctcc
     3061 ctcgataggc ccgtcgaggc ggagaaatgc tctgaaatgg cagaggaact agaggcaagc
     3121 cgacggcttg cggattctac agaaacacgc gtcaacggag gcgaggatcg ataaattctt
     3181 tcggggataa tcttccgacc atccgcccgg ctatcgaccg cataaagatt tcacagtcag
     3241 cgatcaagct ttcggaagag gattgggagc gcgcagaatt cacacgttgc tgctccacga
     3301 gcctggacca aggcggtaaa cgaaatggga ggtggttcat gggcctctgg aacgcagcgg
     3361 tgcatttctg gaaggcgcta tcgaatgcgg acaaaattaa tttcgcaatc gctgtctgca
     3421 cagcgtttgc ggcgctaggc gccgcctact ctgccttgtt ggcgagacgc tcgattcggg
     3481 gcgcggtcga acaattccgt gctcaggcgt tcctcggcgt tctcgcttat gagcgcgagg
     3541 tcaacttctc agtccagatg gacgtgatcc ggtccttgtg tgggaaaccc gccaaagacc
     3601 tgactgaagc cgagcaaaaa agcattctag tcgtggtcaa tttccttaat catgttgcac
     3661 atctgatccg gcactgctac gtcgtaccta agcagatgtt actgctttat gcgccgtcta
     3721 tcgcagcttg tcgggataat ttgctgcagg acggacagtg gctgaaggaa ctacgcaagc
     3781 agacagggga agcgcgatac tacttgcact tcgctaagct gtgccagagt gaaactgaag
     3841 acctcatttg gaaggacatg gcggcaaaga tcgtttggac taccgacccc tacgagcccc
     3901 agctgccgcc agtctaattt ccccgtgtac cccgcgtgta cctcggttgg gcgaatccgg
     3961 tgtttttggc gtttttcgcg tttagtagaa tcaataactt acggattttc agcgtcgctt
     4021 ttagtccgat ccccaccgcc ctgccaaaat ccaaaaatca atttgctgct cgttgttcga
     4081 aattcgtggc tgcaacccag gagccgccag cacgcgcttt cgtccatttt cgcagtacca
     4141 ttccagcgac cactgctgta aactccgcgc actagagggc accagaatgg accgtagaaa
     4201 tttcctgcaa atgtctgcgt tagcggcgac ggccttgccg attcaggggg cttttgccat
     4261 gcaaacagct agccagcaaa cgagaggagc ggcgacgatc atctcgccca aaggggcgca
     4321 ggcatacagc atgatgggcg gcggggaggc tcgcctgctc gtgacgggcg agaagacgaa
     4381 gggcgcatgg tggatgggac gcttccgcga agatcctgga ttcacgaccc agttgcatta
     4441 ccatcccaaa gcggacgaac agttttacgt tctggaaggg gtcctctctg tctacgttgt
     4501 tgatgcgtgg catgaactcg gtccagggac acttggactc ttgccgcatg gaacaccgca
     4561 cgcgcaagcc aatcatagcg acaagcccgt tcatttcctg ggatctgggg cgcctgcggg
     4621 ttttgagaaa ttctttccgg cagtggacgg actcatgaag cgacgactga agccggggac
     4681 cccagaattt gcggccgaaa tccggaagat cgccgctggt tgcgacatgg tgcccctcgg
     4741 accgcctccc aagtaattgc tgctggccta atagaagccc gaccgaaggg caaccgcaac
     4801 gaagccaagg ggctgcggtg tcgaacaagt aggtgaaagc ccgagtaaaa gtggtggacg
     4861 tggccgaacc ggcgacccct gcttgcaaaa ccactttaaa gacactaagt agttgtttcg
     4921 tcttgccttc acctatgtca tggtactcgt tttgtggcgg tgtttggtcg gctttgttcc
     4981 tcgattgttc ccggatgcgt agcgtgcccc gaagtgtgat gggcacagct gccgcttcga
     5041 tggctcagcg gcgcgagcga aaacgatcac gtcatccggc tggggcgtgg gcggactaat
     5101 cagcgtgaac agctgatttt ggatcctgcg atacgtttct taagcggcga tttccgcagt
     5161 tcgttgccgg agcttgtgat aaaagctaca gattgagagg tctgcaggct cggccaacta
     5221 cacactgagt ttttcagctt cttgggaata gtcgctaaag gctttgtcca ttctcacgag
     5281 caacattctg cgattgccta ggtcttgcat tttaagtctc ctaagatttc tccagcccga
     5341 tcacgacgga ttgcggcgca acatccggct ccggaagttc ctggcagacc gttttgctcc
     5401 aagcggccgc gtcgttccgg tctgcttcgt tcggacaatc gatccgaacc cagccatgaa
     5461 tcgaacggtc ggccgggcgc gtgaattgcc cctggtttag cacgatgaaa ttaagatctt
     5521 tgattaacat ctccgagtga taagaattcg cgaaacggac tctcgggagt tcggaggaaa
     5581 gggaaggcga attggatggc gcaacagagt agcggcagac tagcgtccca ttgccgcggt
     5641 tccttaccgt ccaaacttcc aaggcatcta atggaaggtt cgggactttc ctatattcga
     5701 cacgaaccga ggaaggatcg ccgctcttaa ccaggtctcc ccaagaggac acaactgcca
     5761 attctagtgc tcgatcgagt tccattttat ctgcctttca agtgcgccga gtgcgcggac
     5821 cgctccgcgc tgaaagcgtc tcgagccaat tccattcgac cgggcatctt aaaggagtgc
     5881 agcacccagg agacgcagcc ctgaaccgta atttcggtga tattcagctt gtcggcgatt
     5941 tcccgatcgt tgaagccctt accgaccaag cgacagatgg gacccaatac tccgattcgt
     6001 tcccagtaga cacgcatgga ggcacctcaa tgcctgcgag cttcgacctc tccttgaagg
     6061 aagccgcgaa gggacgacca ttccccaaag ggcccgaagc ttttagtaca tcccgcccgt
     6121 cccgccactc tggccagcgc cgaccgcggt catttcttcc tcttgcttga tatccgcgat
     6181 caagacttcg gtggtcaaca ttaaggcaac aatggatgcg gcgttctgca aggcgagccg
     6241 agtaaccttg gctggatcga ttacgccagc tttgatcatg tcgccgaact ctcccgtctc
     6301 ggcattgaat ccgaaatcct cttccttcga gtcacgcact cgcccgacga tgatcgctcc
     6361 ctctcgtccg gcgttctggg cgatttggcg agttggttct tcgagggcgc gcctgacgat
     6421 gttcacacca gtcgcctcgt catcctgaag gttcagcttt gcaagtgctg ggatgcagcg
     6481 aagtagggca acgccaccgc cgggaacaat gccttcctca actgccgcgc gcgtggcgtg
     6541 catcgcgtcc tccacgcgag ccttcttttc cttcaattcg gtctccgtcg cggcgccaac
     6601 cttgatgact gccacgccgc caaccagctt tgcgaggcgt tcctgcagct tgtcctgatc
     6661 gtagtccgaa ttggagtcct ctatctgagt tcgaagctgc ttaatgcgac cttcgatttc
     6721 gctcgctttg cctccaccct cgatgattgt ggtgttgtcc ttgtcaatga tcactttctt
     6781 ggcgcggcca agttcctcga ctttcacgtt ttcgaggttg actcccaaat cctcgctaat
     6841 ggctttcccg ccggtgagaa cagcgacgtc ttccagcatt gctctgcgac gatcaccgaa
     6901 gccaggagct tttacggcag cgcactgtaa agtgccgcgg agcttgttaa tgacgagtgt
     6961 ggcgagagcc tcgccctcga cgtcttccga gataatcagc agaggtttgc tcatcttggt
     7021 cgtctgttcg agcagcggta acagttccct tagggaactg atcttttttt cgtggattag
     7081 aatgaagacg tcttcgagca cgcactccat gcgctcggga gccgtaacga agtacggtga
     7141 aaggtagccg cggtcgaact gcatgccttc caccacttcc agcgttgtct ccatcgtctt
     7201 cgattcctct acggtgatga cgccgtcttt gcccaccttc ctcatcgctt cggcgatgat
     7261 cgagccgatt tgccggtcgt tgttggcgct aattgcgccg acttgggcga tcatgtcgcc
     7321 cttcacgtcc ttgtgcagcc tctttatttc cccgacagcc atttcaacgg ccttttcgat
     7381 gccgcgcttc agtgccatgg ggtttgcgcc agccgccagc gtcctgaccc cttcccgaaa
     7441 gatggcctgt gccagcacgg ttgctgttgt ggtgccgtca ccagctacgt cggaggtctt
     7501 ggacgcgact tccttgacca tctgcgcgcc catgttctcg agcggatccg gcagctcgat
     7561 ttccttcgct accgtcacgc cgtccttcgt gatgattggg ccaccgaatt tcttttcgat
     7621 cacggcattg cgcccctttg ggcccaacgt aatcttgact gcgtcagcga gaatgttgac
     7681 gccacgcaga atcgcctgac gcgaattcac acctgttaca atttgcttcg ccattgactt
     7741 taccctccag ggtgcatgca acctctgtcc ggtcttgcaa tcgttggatc ggagaagcta
     7801 cttcttgctg gatgctgctt tggctatgcc actcactttc gcgaggatat cttcctcgcg
     7861 aaggatcaga tattccttgt catcgatctt gatgtcgttg cccgtatatt tgccgaacag
     7921 aatgcggtcc ccacgccgca cgtctagcgg gattcgaccg cccttcccgt taagcttgcc
     7981 ggcgccaact gcgatcactt ccccttcgtg gggtttttcc ttggccgtgt cgggaatgat
     8041 gattccaccc tttgccgttt ctttctcttc gagcctgcgc accaaaacgc gatcatgcag
     8101 tggcgtcaca ttcatcgtca tttctcacct ccgtttcact cattgttaaa attcaaagag
     8161 cctgataccg atagaattca gactcaaggg cttgccgtat tgcctcgagg gcatccgcgt
     8221 cctttttcac gcgcgcgacc tcgttttgcg gatcggcttc gcgttcctgg cttattgccg
     8281 tggcacttgc cacttcggct tgactgttca gttcctcatt cccagagaaa acgtccggta
     8341 ttttgaaaga tcttgcgccg aaaccacgga agtcgaaaag acgatcagag ccaaagcgat
     8401 catgagcttg tcaaacattt tcatttcaga ctccgcgagg cgattacaga ctcggtgccg
     8461 ttgtcacgct gacatgccgg cccgttcttt cgggatcagg tccttgcgca ctcgtgagcc
     8521 tgctctagct ggagttacag ttgattttgt tttggttgac tcccgagtgc gctcggcaga
     8581 caaaagaacc atggactcct gaacgtggcg cgattgggcg cccacgctca ggtaaggcag
     8641 cccgaggaaa tgcctgcggg ccactcgcgt gatttcaagt gaattgaacg ggctggatct
     8701 tgagttggcc agaattctct tgatcgccct gcgtaacatg aattgctcgt cagtaccgag
     8761 cacaatcgtc ccgatttcac ccgcgaggca gaagaaggtc caccccgttt cgcggacctt
     8821 cctatcgaat gcgtacccgt tgagactttg gaccaatccc caacccggca cacacggctc
     8881 attttcaatc cgcaggcttt caggcagaag tgttccgtcc ttgataagaa cggccccatc
     8941 gcgaatcgcg tctcgcaact ggtcttttct catggccata ctcgatcctt agttgcctgc
     9001 ggactccttc gattcatttc gaggtgaagt tttctgcaac actgacacca agagtcggcc
     9061 tgatgcagga acccgcgcct ccccacccaa cacgatctct cttgctgtga gcttgcgccc
     9121 gtaaacgccc gcggtggcgt cgtcgtccgg acgaagtgtt gaaccttcca gcgaaatgcc
     9181 ggcaaagaga ccacgcgaac gcgagtagct gagtacctcg gcccgcatgt gcgcatccgt
     9241 atttgccgcc gcgtcgcggc ccacgggtcc cgctgccacc gacgcatcgg cgcccaattt
     9301 gaccttgctg ctaaggatgg agctcgcgcc cctcttgttc atgatcaaca gaactagatc
     9361 ggtagcttgg ccgccgatct gaaatccaac gctgccgccc tccagtgcca tcatcgcggg
     9421 cgcgccccac gccccccgga aatctttccc ggttcggcaa accatcgctc cacggccata
     9481 gctcgcgccg atcccgaacg ccgccttgag aacggacgga aatacaatga cgcactcggc
     9541 cttctcgatc acctcttgcg ggatgttttc tggcgtattc ataatttctt gcatcacgac
     9601 ccccgcattg tggagacgct tttgctcttt gtttgccgca aaaaccggag cggctgcaag
     9661 cagtcccaaa cagacaacga gaactgattt acgtatcatc gctgacctcc atgatcagaa
     9721 tttgcggctg gcggaagatt ccgtaacctt tgtgggagca ctattagcac gtcatgagtt
     9781 gagcgtccca tcgtgggtca ggagacgacc ctccggcgcg ggaagaacct gatgagaacg
     9841 gtcgccacgg ccagcaccaa cagaatccgc cgggttcgta agccacggcg actcccacga
     9901 gccacaaaac aaacaagatg atgtccattg tccagagcac attgagcctc ttttcccttt
     9961 ttctttagtg caggtctgaa gcagagactg tcaggctgaa gcgggtgtgc cgatctgcga
    10021 aaacatctct cgtgctcccc atacaagcgg aatcctagac atattcgcaa tctttatctg
    10081 ttctgttgtg cacacatttt taattctgct tttgcaactg tttcgcgttc cctgcccgcc
    10141 tagttagcga gaggctcgac ctagtttggt tttctcaact catttacgag cttcctggcc
    10201 tctttcattc tgtccttgta ataggctggg ctgatgagaa cttctttgtc tcccgcttgg
    10261 acgcgctctt tgattcccgc tttcgacagg aacagggcta gtgcgatgta cgtcttgatt
    10321 tccttttcta gaaactgaac cgcttgtcgt ttggactcgt ccatttctca cctcgaactg
    10381 ccgctaattg caaagctgag agcgatgagg cccgagtccc gccgggcccc ggttgattcg
    10441 ctcagcaaca ttccacgtcc gcgccctaga tgacaccgag aagcacgaaa ataatgatga
    10501 cgaggagaag gagactgact ccacctgtcg ggccataacc ccatttccgg ctgtgagacc
    10561 atctgggcaa cacagccaaa agtgccatca ccaaagacac gatcagtatc gttccaagca
    10621 tcgttcctcc cgttccagaa ggcggtggtt tcggcgagag ccggcctcaa tcacacgcca
    10681 tcgcgctact cgatatagac ttggaagatt aaggaagggt cactatatgc aacggcatac
    10741 catagcccat ccgttcccat agtgaagtac acgtcctgct ggtaaaacca cgctggatag
    10801 gctccagcgt agaaccaata tccaccgtca gagtactccc gacgcccgtt gtgatagcga
    10861 ccaccatgct cctcaaagcg ggcatgatgc tgaggcccaa acctactttc ggtgtactcg
    10921 tgaccgcgac tatccttctg accattgcgg gcccacttcc cttgcgcgtg ctccggctgc
    10981 tgaccatgct gacccctggc gtgctgctct tgatcctgcc gtgtggccgt aacctgtttc
    11041 acttgttgct ggttatgcga cttggttcgc tcttgccggc tcttggcctc ctgttgctgt
    11101 tcttgccttg tagacttagt ttgttgctgt ctctgttgct gctcctgctt accttcggct
    11161 tgttgctgcc tttcaggttt cgcctgctgt ccttgtctcg ctggctcggc ctgtcgttgc
    11221 ctctcaggct tggctttctg ctcccgtttc ggaggtttag cgtgttgatc ctgccttgcg
    11281 tacgtgggag cggcggctcc aagaagaagg agcagaacac tcgcgccaat aactcgaatt
    11341 gtcttcatgt ttgttgcctc ctaaggcgac ttctggcgcc actggttcat caacgtcaag
    11401 cgcgaatgtt gtttccggtt ctcggtaggc gacgtgcgga tctctcctat atggttcatc
    11461 cgggttcgct ctaagtccca acgcggatgt gggagagaga gtggaaaagt ccaaaaatgt
    11521 tcagaagcca cagcactaca cagatcacca cgactgcatt caaaatggac ttgatcgttc
    11581 cctgcattgg cacgtacctg ttgaccagcc aaagcaggac acccaccacg atcagaacca
    11641 tcagtatttg aagaataggc atttcactat cccttctctt cgcaggcaca cacttcaagc
    11701 cggtccaaag gcgttctgca ccaaatttct gtggtgaacc gagaactctc agtgtcggct
    11761 gaaatgggaa ggaacctgca atctccaata ttgccgaggc gggagctctt tgactcgttt
    11821 ttgccttggt gctctcgctt cgcccacaag ccaacatctt agaggaggcg atggacaggc
    11881 gtctgttcac tagtgcacag atctgccctg ctgtgggtct gcggcctcgt gctaagatga
    11941 ccagccagga gaacgattat ggccgtcaca acgggagatc ccaatcgtcc caaagccaac
    12001 cgtaagcacg ccttcaacgc tcaggatttt ctcgattcgg cgggcatagc tcggagagtt
    12061 gtggagttta agggaaaaca agcgatcttc tcgcagggcg accgcgcgaa caacgtcctg
    12121 tacattcaaa aaggcggcgt gaggctttcg gtagtgaatg aaaccggcaa agaggccgtt
    12181 gtagcgattt tcgggccagg cgactttttt ggtgagggtt gcctcgcagg tcaacctatt
    12241 cggatggcga cggcaaccgc ggttatgttg accactgcac tggtcattga gaagaacgag
    12301 atggttcgcg tccttcacgc ggaacatgcg ttttccgacc gtttcatttc ttacatcctt
    12361 tcgaggaaca tccgggtcga agaagacttg gtcgatcagc tctttaattc cagcgagaag
    12421 cgactggctc ggatgttgct cctgctcgcg cgctacggca agcaagataa acctcagagg
    12481 gtgctcccca aaatatcgca gcagatgctg gcggaaatgg tcggtacaac acggtcacgc
    12541 gtcaatttct tcatgaacaa gttcagaaag ctgggcttca tcaaatataa cggcggtctt
    12601 cagatcaaca gttctctctt gagcgtcgtg ctgcacgact gatccaagga acgatgcttt
    12661 taatcgggcg agattgactg acgagcgccg aaaaccagtt tcaagagaaa atccttgggg
    12721 cagccaagcc gacccctcgt tgaatacacc cagaatgagc cgtgacactc ataacaggca
    12781 tgcccaggaa ccgtttcgaa atgactttcg tgaattccag cgagttaaac ttgaagcttt
    12841 gctcctcttg tctggctaca acacacttcg ctgccttgcg cagagctccc agatctttgc
    12901 ggcagaacac ggttgctcgg atctcgcccg ccagatacga aaacttcaag ttcgctccca
    12961 caacttcccg gattagggct cggtgatctg aattcctgat aatccgccag ccgggtaaga
    13021 aagcttcgct tgccagcgaa accgccgatg gcagcggtgt atcttccttg atcaaaatga
    13081 ttcccgtccg attgatgcgt gacacttgag ctctcgcgat cttcccggtc gccactcgcg
    13141 aacgcgaaaa gagagatgcc aaaacgcttt tttgctgaag acggccggat tcaatcttct
    13201 ttacagttcg caagattgat tgacaccacc gagacgcgcg ggcgatgaaa ggccatgcga
    13261 acaacagacg atcgcaattc tcgtaacgta gtggttgttc aaacaacttt gcaagaagac
    13321 ttgattcgag aacaactcgg cgcctcgttc tcttgattcg gctctgagga atcagaagct
    13381 tagtcaacac agttgtactc ctgataaaaa gtcgtctgcc agctatcgtg cggcgattcc
    13441 taccctccgt ctgttctctt tggcacagtc cttagctaga aactgtggag acgtgtccgg
    13501 acgtgggtgt tcgtaactga gcagttttaa aaatgaaatg ccttaaggtc gcaagctcac
    13561 ctcggagggg ggcgccatgc caaaggttcc gtcgcgaagt ttgaaacgtg tatcgaattg
    13621 aacagtctcg ttacactgat atctgtacca ttctcgcgtc cgtgatttcg tcaccactgt
    13681 agaaactgtt ttcccgggaa agcgtgcccc gttccgaatc tcggaaaaga aggaaaggaa
    13741 aacaagatgg ctcaggctct ctctcagcgg ctaccgacat cagaaatccg cgtcctcaaa
    13801 atgctctcgg agggtgctcc aatggcggaa gtcttgaacg aactctgcaa ctttattgat
    13861 gctaaggcgc ccggggtgat cccgaccgtt ctattgccgg acggcgatgg gcgtctccgt
    13921 ctcgccgccg gccccaaagt tcctaaaatc tggaacgaag cgttcgacgg catggccttg
    13981 cactcgtatg tcagcttcct ccgcgcggct ggacgtgggg aaggccccgt cccagttgcc
    14041 gatataacga gcgatccctc atttgcagct tgctgggacc tggctgttaa tcaaggtgtc
    14101 cgagcggcgt ggaccgtgcc tattttgtcg aaggagagga agatcctggg aactctgatc
    14161 ctgttttatc ccacgcctca accgccaggc cagcgtgacc tggaactcat ggagcgggtg
    14221 atccatatgg cggcaatcgc gatcgaatgt caccgtagtg aggaagagct acgggagttt
    14281 tccaggcggc tttatcaatc tcaagatgag gaacgccgtc gtattgcgcg cgagctgcac
    14341 gacagcacag gacagaagct tgccatcctg gaaatcgcat tgtcgaggct aaaccgtgtg
    14401 gtaaacagcc gcccgcggga atccgaggag attctcctgg aatgtgcttc tctcacgagg
    14461 aacatttccg atgaactccg aactctttcc tacttactgc atccgcccat gcttgacgaa
    14521 tttggtttga actcggcaat ccggtgctac gtgaatggaa ttaaccagcg caagggtctg
    14581 catgtggaag cggaaatatc tcaggagttg ccccgcctgt ctgaagaagc cgagcttgcg
    14641 gtctttcgga tcgtccaggc cagtctgaca aacgttcatc ttcactccgg agtagcccta
    14701 gcgaaggtaa agattgagca tagccccgat ggaatcgtcg tagtaatcag tgatgaaggt
    14761 cgagggatac cggatggggt gctggatcat tcgtcgcagc caaagacagt tggcataggc
    14821 atcacaggca tgcgagaaag agttaggcaa ctgggcggat gcatgcagat tgaatctagt
    14881 gcgagtggca caagcgtaaa ggtaacagtc cccagccgtc attttcgaac cgctatctca
    14941 gccgcagaca aacgcacgtt caggtgtgcc gctgataacc gtgaactgca ttccaactaa
    15001 gccgaaacaa atttgctctg cgactcggtg gagaaatgtc gctttcgcgc ggctgcgaga
    15061 gaaccttggc gcagtggacc ttgacctgac caccacgctt gaagtctaca cacacgccag
    15121 cgcgagcgcc taaaggcacg ccgtcgagca gttgcaaaat caaatgttcc caaatgttcc
    15181 cgggtttgcg gaaatcactg gaaggccgaa ttagtaagtg cctgaaattg caggcggatg
    15241 gttggtgtgg ccgggatcgg actaaaagcg acgttgaaaa cgcgtaagtt attgattcga
    15301 ttaaacgcga aaaacgctaa aaccgctgat tcgcccgacc gagatacacg gcgggtacac
    15361 aacgctggct aaagccgtga tcgattgggc gaccgtcagc tcgggcgttc ttttgcgaag
    15421 gccccgccaa agctggcagt ggcgacgcaa gcccattgag tttcacgatg cggctcacct
    15481 gtgagactgt gtgaaaatcc attaacaatt ggcggtcacg cttgctcgtc gataggtcct
    15541 tcgttatgct cgcaataccg tgaatgctct ctcacgaatc ctcatcattg aggacgataa
    15601 ggacatcgtg gaacttgtcc gatacaatct cgagaaagaa ggttttcaag tgcttgcttg
    15661 cggggatggt gcgagcggct tggcgcagat taagaaatcg ccgcctgact tgctggtttt
    15721 ggacctcatg ctccccaaac tatccggatt ggaaatctgc aaggagattc gacgagacaa
    15781 caggttgaat cgtctcccta tcctgatgct gacagcgcgc ggtgatgagg cggaccgtgt
    15841 ggttggactt gaactggggg cggacgatta cgtcacaaaa ccgttcagcc ctcgcgagct
    15901 tgtcgcgcgt gtgaaagcgc ttcttcgaag gctggagtgg ccgagcgatg aggctcgcgc
    15961 ggttatagtg ggattgctgc gcattgatcc ggtttcgtac cgagctgaaa gtgaaggcag
    16021 gcaattgcct ctcagcacgt tagaatttcg attgctgtat tacctcgcat ctcggcccaa
    16081 ccgcgtcttt tcgcgcgacc aactgctgga tgccgtgtgg ggcagcgagc ggtttgtgac
    16141 gccgcgcagc gtcgatgtgt acgtccgccg gttgcgcgaa aaagtggaaa agaatcctga
    16201 ggagccagaa catctgaaga cagttcgcgg cgcgggttac atctttgaaa cgaaagtgaa
    16261 ctgagcgcaa ttgaaagtgg ctcgcagcgg tgtcttttgg aaactcgggc tgacctattt
    16321 tgttctttta atcgctgtcc tcgccgcagt tgatttttat gctggccgaa tccttcggca
    16381 aaattacatt cacgccgcag acgagcggct aaattctttg gcgctgatcg cgcggtctaa
    16441 cccgcctaag ttcgacgacg aaacggcatt agcatcctgg gctgccggga tgtcgcgtag
    16501 cggagcgcga gtgacgatta ttgctgccaa tggccgggtg cttgccgatt ctgcccacga
    16561 tatcgaaacg atggaaaatc actcgaaccg tccagaattt cgtcaagcca tgagttccgg
    16621 cgaaggggaa tccgtgcggc acagtcgcac gttggatcgt gatttggtgt attgggcggt
    16681 gcggttcgaa cgtccgtccg gttcacccgt tgtgattcgg ttcgccacgc cgcttgcgga
    16741 aatcaacagc gcgctagcgg aggtgcgtcg aagactgtgg gaggtttcgc tggtgatttt
    16801 gctgttggcc atcggcattt ccgtgctttt ctcgcgacgt tttgcgaagc gtgttggcga
    16861 actccaagaa ttttcagaac gggttgcgac cggcgacttt cgtccgctaa tccaggaatc
    16921 cgccacagac gaactgggag atttagctcg cgccctcaac gacacagcct cgcgcttgag
    16981 cgcaaccatt agctcgcttt ccgatgagcg taatcgtacc gctgcaatac tgcgcagcat
    17041 ggctgaaggt atagccgtaa taaatgctga cgagcagatt acgttctaca acgaggcctt
    17101 cgccaggctg tggaatctcg ataatcgaag ccacgcaaac cggtctgctg tatccttgat
    17161 ccggcatccc gatgttctgc aattgattcg cagcgccctt gcgggccaag aagcgcgcgg
    17221 agagctgtct ttgggctccg cacaaccaag acacttttct gtgaccgcgg ctacgattct
    17281 cgcttcatcc gatggatcgg cagactcgga gaagcaatcg aagcctgccg gcgcggtggt
    17341 cgtgcttcac gaaataacgg agattcgccg gctcgaacaa gttcgcaagg atttcgtggc
    17401 caacgtatcg cacgagttgc gcacgccgtt gacggctatt caaggctttg ccgaaacgct
    17461 tcttaatggc gcgcttgacg acccacaaaa taaccggcat ttcatcgagg tcatacgaaa
    17521 tcacgcaagt cgtctggcgc gtttgacaga ggatcttttg gagttatcga agatcgaagc
    17581 aggcaaactg gcaatggatc tgcgtagtgt agctgtagct gacctaatcg cgcctgtcat
    17641 tgatttggct caggaccttg cgttaagaaa gccagtctct ttgaatgtca tcaatccggc
    17701 gattcagttt ccaatggtta ggggagatgt agggttgttg cgagaggttc tgcgcaactt
    17761 gctggacaac gccattcagt acactcctgc cggtggacgc gtcgaagtca gtgcagaacg
    17821 taacggttct tctgtcgttt ttactgtcgc cgatacaggc atcggcattc cgcaagcgga
    17881 acacaacaga attttcgagc gattttaccg cgttgacgct gcacgttcac gggaagcagg
    17941 cggtactggc ctcgggttgg cgatagcgaa gcatatcgtc gaggcccacg gcggccagat
    18001 ttggctggat agcgaaattg gcagaggctc gaaattccat ttctcaatcc cgctcgccgg
    18061 gtagcgttcc ctaagaattc atccatctgc cattctcccg caataagtgt caggcatgct
    18121 ttttgacagc gcagtcgatt tggctgccgt cgcggttaag gagagagcat ggccacatca
    18181 gcccgcagtt cgtcggattt ctatactaca gcccttgaaa actatctcgt tgccatggcg
    18241 cgaaccgtgg agggctccat aaatcgttct ttcgaagcgc tgctctcgcg gaatgctgag
    18301 attgcgagcg ccgtatttgt cacggagcca aaaattaatg aatcggaaat ccttatagat
    18361 gagcaggcag tgcgactgct gcaaaggggc gacctgtcac aaggcgatgt gcggcatatc
    18421 gttgctactc tgaaaatcaa caacgacttg gagcggatgg gcgatctggc cgtcaacacc
    18481 tgccagtgcg taatcgcgct ctgccaatcg cccagtgcgg acacgccgcc tgaattggca
    18541 tcgatggttc ctccggtaag agcaatggtt ggccgctgtc tcggcgccct cactcatcga
    18601 aatgttactc tcgcatttga ggtgctccaa aacgaagaag cgattgatcg gcagcgtgac
    18661 agggttgttg agatgctgat ctcggcagtg aagaaggaac cttcgctggc aaccgtcaac
    18721 atccaataca ttcttgctgc tcggcacatg gagcgcattg cggatcactg cacgaatatc
    18781 gcggaggacg ttatcttctg gttgcaaggg ttagacatcc ggcataatcg aaacagcccg
    18841 tccaatccac caagcgtcga atgacgtaac gcaatattca tctgcaccta atcccagagt
    18901 tacagcctct ccgcatactt accgcgctcg gatggccgaa ccccatagct gcggtttgga
    18961 tacaccgcaa ccaacaggag aactgaatga ggcgaagact tagcgctctg attgttgcag
    19021 ccattttccc gttagtagct gcatctgctc agacgaaaac gaataacgat gcgaactcat
    19081 cggcgaagga gaactcatca tcagcagcgg tcaggtcgaa cacaaaaacg gcgacagcag
    19141 cgaaattgtc caccgctgaa aagtctgtga ctcggtacgc tatttcaccc gtttccgcca
    19201 aatcctctga cgccgctaat gctgcggcgc aagatacgga tgaaaaccag ccgccactct
    19261 cgttcaaggt tggtggcgcg gacttcaccc ctgttggctt tctcgacttc accaccgtat
    19321 atcgctcgac gaatgtcggc agcggaattg gaacgagctt cggcagcata ccgttcaatg
    19381 gctcttcgcc agccggacgg ctgtcggaaa cgcgcttcag tgctcaaaat tcccggatcg
    19441 gtctgcgagt ggatgccaat ccatccgcaa acctgaagct aacgggctat ctcgaagcag
    19501 attttctggg caatgcgccg gggaatctcg aagtcacaag tcattccgat acgctgcgca
    19561 tgcgtttgta ctgggtagat gcgcattacg gcaaatggga atttctcggc ggacagtcct
    19621 ggagcatgat gacgcccaat cgcaagggca tctcgcccat gccttccgat attttctaca
    19681 cccaagacat ggacacgaac taccaagcgg gcttggtgtg gctgcgcacg cccgggttcc
    19741 gtgtggtcta tcacgcggat gaccgttgga cgctcggatt agccctcgac gaccccgagc
    19801 aattcactgg cggcttggtg attcttcctg cgggcttcaa cgcagcacaa gtagacgatt
    19861 ccggcggaaa tgccaagacg ccgaatgctg ctcccgatgt tatcgcccaa attgcatacg
    19921 acacgagtct gggcagccat gcgtttcaca tcgaagctgc gggactgtat cgcgcgtttc
    19981 gcttgaacac tgcggcaacg aatgcgaacg tcacgcgctc gggcggcggc gggtcggtga
    20041 atgtgaattt tgagatcgta aagaaccttc gtttgatggg cacttccttt tggagcgatg
    20101 gcggcggacg ttacatcttc ggcctcggcc ccgatttcgt ggtgaaaaca gatgccgctg
    20161 ggaatttcat tccctcgccg ctgcacgcgg gcgcgggcat cggcggcttc gaatatcagt
    20221 tgatcccaca aacgatgatt tatggctatt acggtgccag ttatttcggc agggactttc
    20281 aagaagtcgc cgctggttct ccaccgccct tcgtcggata tgggttcgcc ggttctaaca
    20341 gctcccagaa ccgcaccatc caggaaggaa cggtcggttt gatcgaaacg ctggaaaaat
    20401 cgcaattacg gcgatttgaa gatcatcacc caagtttctt acctttcacg caatccatgg
    20461 tcgaatgccg gtactcctgc tggggcaagg agcgcgcatc tcggcatggg ttttgtggac
    20521 ctgcggtatg acctgcctta gaactgcatt cactcaatct tcaagcagat gtaacagcac
    20581 tgtaacaatg cgcgggcaaa acagtctgga ggataaaagt gacgaagctt gctggccaga
    20641 gttggttggt tctctgcacg ctgctgatcg gagtttcatt ggtgtcctgc tcgcataacg
    20701 tgggcaccac aactggttcg caaaccgttc tcatcaatgg cgctggttct accttcggtt
    20761 atccgattta ctctcgctgg gcggatgact ttcacagaat gcatccgaat gtcgaagtta
    20821 actatcaatc ggtcgggtct ggggctggta tcaggcaact gctagcgggc acggtcgatt
    20881 ttggcgcatc ggacgcaccg atgacagacg cacagcttgc gcaagccaaa tcgccaattc
    20941 tgcacttccc gactgtactt ggcgcggtcg tgccgaccta caacattcct ggtgttaccg
    21001 cagagttgaa atttacgccg gaagcactcg cgggcatctt tctcgggaag gtcacaagat
    21061 ggaacgatcc tgccatcgca cgcgtgaaca ccggactgaa attaccgtcc ggcaatatag
    21121 tcgtcatcca tcgttcggat ggcagcggca catcgttcat ctggacggat tacctttcca
    21181 aagtaagtgc ggagtggaaa gccaaggttg gcgctggaac ttcggtaaat tggccggtcg
    21241 gactaggcgg taagggcagc gagggcgtta gtggtttgat tcagcagacc ccggattcca
    21301 tcggctacgt ggaactaacc tatgcaattc aaaaccacat cctttatggc tcagtgcaaa
    21361 attcatcggg agcattcgtt aaggcgagcc tcgatagcgt aactgccgct gccgcatccg
    21421 ccgccgattc gatgccaaag gattttcgcg tttccatcac caacgcaacg ggcaaagatg
    21481 cctatcccgt cgcgagcttc acgtggcttc tcgtgccggc gcagatttct gatccaacaa
    21541 aacgcgacgc aatcaagagc tttctgcact ggatgctgac gaaaggacag aatgatgcag
    21601 agccgctggc gtatgcgaaa ttgccagccg acgtgagtac gaaagaggat gcgacgattt
    21661 ctgagattcg gtagctatgc cggcacccgt gaatgtcttt gatctgacgc cctcgtctcg
    21721 acctttgcgc gtcttctggg atcgtttgcg aagaggcgac gaaatcgctc acgttatgac
    21781 attgatttct gcaagcacgg ttgtaggcgt caccgctctg cttgtatgcc agatgtggat
    21841 tcaatcggcc ttatcgcgcc agaaatttgg ctgggcattc tttgcaacgc gtgtttggga
    21901 tcccgtggcc gggcaattcg gggcgcttcc attcctatac ggaacggtgg tgacttcagc
    21961 aatcgctcta ttgctcgcag tgccgcttgg cctgggaacg gccattttcc tctccgaatt
    22021 ttctgtcagg cgcatatcaa gcgtgcttgc atttctcgtg gatttgctgg cggcggtgcc
    22081 gagcgtaatt tacgggctgt taggaatctt tgtgttggct ccgtggctac gcgtctcggt
    22141 cgaacctatg ctgagatcgc atctcggttt tttacctctc tttcgcggtc ccgcatacgg
    22201 cgtcggtttt cttgccgcgg gactgatcct tgcggtcatg gttgtgccat tcatcgtctc
    22261 tgtttcgcgc gaagtattgc tttccgtgcc tcgcgagcaa cgcgaagctg ccttagcttt
    22321 gggagcgacg cgttgggaga cgatttcgaa ggccgtagtc ccgtttgcga aaaccggtat
    22381 catcggctcc gtgtttctcg cactggcgcg cgcacttggc gaaacgatgg cggttacgat
    22441 ggtcatcggc aatgatccca agatcagcgc atcgcttttc gctccgggct attccatcgc
    22501 agccgttatc gccaacgaat tcacagaagc tacaggaagc ctttacctga gtgctttgat
    22561 cgaattgggt ttggtgctct tcctgcttac atttgtgctc aacggtttcg cgcggctgct
    22621 tgttctggcc actgctcaac gaggggctgg agttcagcaa tgagtcttcg cagtggatgg
    22681 cggaagtgtc tgaacgcatc catgctcggc ctcacggctg tgttcgctgt cgcaacggtc
    22741 tcggtgctgt ttttcatcct cggctatctc gtttaccacg gcggaaaaaa cgtaagttgg
    22801 aatttcttta cgagactccc caaacctgta ggcgagccgg gcggagggat ggcaaatgcg
    22861 atcgtcggca ccgcaaaact gctgtttgtg gcactgctca ttggcgctcc ggtcggagtt
    22921 ttgtcaggcg tctatctctc cgaattcggc ggcaagaccc tgagcttttt cgttcggtat
    22981 acggccgatc tgctcaatgg cgtgccttcc attgttatcg gaatttttgc ctacgcgatc
    23041 attgtcgtgc ccgcgcggca tttttcgaca ttcgcgggag gttttgccct tgggatcatg
    23101 atgattccga taattctgcg gagcacggaa gagttcctca aagctgtccc cagcacgttg
    23161 cgggagggtg ggatggcttt aggcgtgagc aaatggcgaa cggtgctgac attagttgtt
    23221 cctgccgcca gttcaggaat tatcgcggct gtcatgcttg ccgtggcccg tattgccggc
    23281 gaaacagcgc ccttgctttt cacggcgttc agcaaccgct actggagtcc cggctttaac
    23341 gagccggtcg cgtcattgcc cgtaatgatt tacacgtacg caatcgggcc ttatcccgaa
    23401 tggcacgaac aggcgtggac ggcgggtctg gttctgctgg gattggttgt cgtcctgaat
    23461 gtcgcaacgc gacttatttt cgcgcgcaag atggccgctc tcagatagaa gcgtacgcga
    23521 acatgcgtgg agcatccatt gtggagaaac aacaaaatct cgtgcccgca tacgtccgaa
    23581 agggcgacgt tccaacgatc gatttggcaa ccggaaggct aaatccccga ttcacgatat
    23641 cgggcttgaa agtctcttat ggcgctaatg aggtcctgca cggcattgat ctgaaggtct
    23701 tcgcgaatgt ggtcacagcg gtgattggtc cgtcgggatg cgggaagtcc actttgctgc
    23761 gttccttaaa tcgcatgact gaagttgtgc gccgcgctca cattgagggc aaaatcctcc
    23821 tcgacgatga agagatcgca cgaatggatg tcgtgaaatt gcgccgccgc gtgggtatgg
    23881 tgttccagcg gcccaatcct tttccgaagt ctatctttga gaatgtcgcg tacggactgc
    23941 ggatcaatgg gtataaaggt ggactttccg atgctgtcga gcatagcctt cgccgtgccg
    24001 cgctatggga tgaagtgaaa gaccagcttc agaaatccgc ctacgccctt tcgggggggc
    24061 agcaacaacg gctgtgcatt gctcgcgccc tcgcggttga ccctgaggtt ctgttgctcg
    24121 atgaaccttg ctcggcgctt gacccgatca caaccgcaaa aatcgaagag cttcttttcc
    24181 agttgaaagc ctcgtgcaca atcatcatcg tcactcacaa catgcagcag gcggcgcgcg
    24241 ccagcgattt caccgctttc ttgctcgaag ggcagctcgt cgaataccaa tcaacgagca
    24301 agctcttcac aacgccgtcc gacccgcgca cagaagcgta catcacgggc cggttcggat
    24361 gagcaatcat caaatgtgaa ttggggagtt tcaatggaac ggcattttga acacgatttg
    24421 gggcaattga aagagcgcct tttggtgatg gcgggccttg ccgagcgcgc tgttcaccaa
    24481 tcggttgagg ccgtctttga gaaaagcgag catctggcga atcgcgttct cgaagaagaa
    24541 ccggccataa acaaaatgca gatcgaaatt gatgatcgcg taatgcagct tgtggcgctc
    24601 caggctccta tggccgttga cttgcgattt gttctttgcg cttcacgcat caacaacgat
    24661 ctggagcgca tcggcgacca ggccgttaac atcgcgcaat ccgcgctgcg aatcctgcgt
    24721 catccgcaag tgaagcccta cgtcgatttg ccgcgaatga gcgagctggc agagggaatg
    24781 gttcgcgaaa gtctgaatgc cctggttcat ggtgacgccg agctcgccaa atccgttctg
    24841 atccgcgatg atgaagtcga tcatttgcgc gatcagattt tccgcgagct attggcctat
    24901 atgatggaaa actccgccgt tgtctttcct gctttcgagc ttgtcctgat caccaaaaat
    24961 ctagagcgca ttggcgatca cgccacgaac atcgcggagg acgttatata tatggtcgcc
    25021 ggtcgcgacg tgcgccatct ggccgcggat cgccgatgaa tcgttcctca gtagatcgta
    25081 attgaagatg ttgccgcaca actgagagcg ctctggctcg aacgtagctc aatcggtgtg
    25141 cgcgaaaggg ccaggtggaa ttgaagagtc tatccgaggt cataccttcg ggggtcctcg
    25201 tttctcggcg ggcaggcacg cggagcaggg gcatcgctcc gcgatacggg tcccgcaaca
    25261 ctcggctaaa agttcgagcg tcgtgccgtg cgaactgtcg tcggcatacc tatgcgcttc
    25321 gttcgcaagt tggcgcgtct aggccgaact tcaaactgca cgccttcggc ttcctgcaaa
    25381 tacttcacga cctcaaacag attccgcgct tgagaattac agaacggcat tacgcgccat
    25441 ggacgcgatc tcgtcaagag cagatcgaag ccggcctcag agccgcttgg agcaacgatc
    25501 cactcattaa agaggtacac gccgggtaca cggcgcgacc cgccgccaga actcgcgtgt
    25561 tttcaaggag aaggattggt aggggcgatg gggatcg
//
LOCUS       DATNDT010000462         5459 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_695218, whole genome shotgun sequence.
ACCESSION   DATNDT010000462 DATNDT010000000
VERSION     DATNDT010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5459)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5459)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5459
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_695218"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1048
                     /locus_tag="VN788_15315"
     CDS             32..1048
                     /locus_tag="VN788_15315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HXU21951.1"
                     /translation="MVISEHGGSEVLHYTDVPEPAIGAHDVLVRVRACALNHLDLWVR
                     RGLPGRSIAFPHIPGSDISGEVAKVGESVSNVRVGERVLLAPGLSCGQCAQCIAGRDN
                     FCKQYTLFGSGVHGGYAEFVKSPAANVIPIPGNLSFEEAAAIPLVFLTAWHMLFYRAH
                     LQPAEEVLVIAAGSGVGSAAIQIAKATGARVIATAGSQPKLSKAKTLGADEVLLHEDE
                     FAREVKRLTNGRGVDVVFEHVGAATWDQSVYSLAVGGRLVTCGATSGFDSKINVGYLF
                     ARQLSILGSFMGHKAELFSVLELFKRGLLRPVIDSVLPLERAAEAHKRLENREQFGKV
                     VLFV"
     gene            1301..1675
                     /locus_tag="VN788_15320"
     CDS             1301..1675
                     /locus_tag="VN788_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011367196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HXU21952.1"
                     /translation="MKNLFVGNMSFQTTEGDLRAIFEPFGQVARVHIAMDRETGRSRG
                     FGFVEMPNDEEAAKAIAGLDGKAVGGRNLKVNEARPKGESSGPRGPRSGGSGDRNDRG
                     NRGRFSNEDYREAARQPREPRW"
     gene            1797..1937
                     /locus_tag="VN788_15325"
     CDS             1797..1937
                     /locus_tag="VN788_15325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21953.1"
                     /translation="MKAERRAQKKLEPRNNEEDELKVLTGPAPLEDDPLADPSDWGPS
                     GA"
     gene            complement(2063..3070)
                     /locus_tag="VN788_15330"
     CDS             complement(2063..3070)
                     /locus_tag="VN788_15330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HXU21954.1"
                     /translation="MKMDFGVIDHVDRQKKPIHETYDSRMKLMELYDQAGFSTFHVTE
                     HHFTPLGLAPSPMVFLAAASRITKNIRFAPLVLIATLYNPLRLAAEICMLDHLTNGRF
                     DIGTGRGVSGVELGFFNVKEQEAPAIYGEAMQVLMAALTQDVVDYHGKYFEFTNVPIE
                     MKPLQKPHPPLWYATNAPESAARAARQNMNVVNLLGAPEARAVICAFKDSWNAAYGGK
                     GLKMPKTGIARNLYVGENDRQAEERGKFGSKGFYDSLVYLWRKYNVTAMSLEEVLRAS
                     EASLIAGTPATVRAKIEEQLDQSGANYFVARFAYGELTHEESVRSLELFRDEVMPHFQ
                     K"
     gene            3533..4774
                     /locus_tag="VN788_15335"
     CDS             3533..4774
                     /locus_tag="VN788_15335"
                     /inference="COORDINATES: protein motif:HMM:NF015413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HXU21955.1"
                     /translation="MLHATEVLGAQTYDSAGNFVGRVKELFIVPAEQPNRVSRLLIGR
                     GKYRSLIARYDQIGTVEPGRVNLTADESALELYHPNEAWLALRKDLLDQQIIDTNGRK
                     VVRVNDVDMADQRTNGNTELRLTQVDVGLQGAVRRLLQGLVPPTGIRRLDNNVRGRSI
                     RWEFVNLIEPDPLRRVKLRLSSDKLAELHPADIADIVEEVSPAERQSILDSLDEETAA
                     GTIAELDKRLQTQVVEKLDPEKAADIIEEMNPDEAADLIQNLEPETSKEVLEEMDHRE
                     AREVTELMRFDQNTAGGMMNTEIVVVGEDATRGEVVDYIRFHEMALDQLDNIVLINRD
                     AVLAGTVPLARLILASGEQRMAELAFEPLLSVRPDAKDNEVFELFDKYNLRSLAVVDE
                     SNCPLGTIAVDDVVSRLRAKI"
     gene            4943..>5459
                     /locus_tag="VN788_15340"
     CDS             4943..>5459
                     /locus_tag="VN788_15340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HXU21956.1"
                     /translation="MAVVGPGLITSNVDNDAGGIAVYTTAGAHFGYALLWSLIPMTIA
                     LYVCEEMCARMGVVTGKGLSDLIREEFGFRSTFFVMLAAFAVDLSNTVAEFAGVAASM
                     QIFGISKYVAVPIAAIAVWILVVSGSYRQVEKIFLAACTLYLAYVVSAVLAKPNWISA
                     AKGVVPSVHMNS"
BASE COUNT         1138 a         1481 c         1670 g         1170 t
ORIGIN      
        1 ccgctgatct ctggaggttt cgttgaaggc ggtggtgatt tcagagcacg gcggttccga
       61 ggtgctgcac tacacggatg ttccggagcc ggcaatcggg gcgcacgacg ttctggttcg
      121 cgtgcgtgct tgcgcgctga accatctgga tctttgggtg cggcgcgggc ttcctggcag
      181 gtcaatcgcc ttccctcata ttcctggcag cgacatatcc ggcgaagtgg cgaaagttgg
      241 tgaaagcgtt tcgaatgttc gagtgggaga aagagtcctg ctagcgccgg gtctttcctg
      301 cggccaatgc gcgcaatgca ttgcggggcg tgacaatttc tgcaaacaat acacgctctt
      361 tggatccggc gttcacggag gctatgcgga attcgtaaaa tcgcctgccg caaatgtaat
      421 tccgatccct ggaaacctca gcttcgagga agccgctgca attccgctgg tttttcttac
      481 cgcctggcac atgctgtttt atcgtgccca tctacaacct gccgaagagg tgctggtgat
      541 tgccgcaggc agcggcgtgg gcagcgccgc aattcagatt gccaaggcaa caggcgcccg
      601 cgtgatcgcg acagccggct cacagcctaa gctctcgaag gctaaaacgc tcggcgccga
      661 cgaagttctg ctgcatgagg acgagtttgc gcgcgaggtg aagcggttaa cgaacggccg
      721 gggcgtggat gttgtcttcg agcatgtagg cgcggcgact tgggaccaaa gcgtttacag
      781 cttggcggtg ggcggacgcc tggtcacctg cggcgcgacg agcggatttg acagcaagat
      841 taatgtgggt tatctcttcg cccggcagct ttcgattctg ggctcgttca tggggcataa
      901 ggccgaattg ttctcggttc tcgaactttt caagcgcggc ttgcttaggc cggtaatcga
      961 ttccgttttg cctctggagc gggcggccga agctcacaag cggctcgaaa accgggaaca
     1021 atttggaaaa gtggttctgt tcgtttagcg cgctgtgctt ttcggggctg tgggtcaggc
     1081 gatcgctttc ggcgatctcc actggttcga atcggcctgg tttgtgctaa attatacgga
     1141 cgggctttga ggaattgggc actcgggttt ggggtgtccg gcgggtttct cgaagcttgc
     1201 gtccggcctg ccccagcttc ggatcgagat tcgggcattc ccttgggcca gttgtggatg
     1261 aacagccgaa gcttggacga gcgtgaagga gaagagcggc atgaagaatc tgttcgtggg
     1321 aaacatgagc ttccaaacca ctgaaggcga tctgcgagca atcttcgagc cgttcggcca
     1381 agtggcgcga gtacacattg cgatggatcg ggaaacgggt aggtcgcgag gtttcggttt
     1441 cgtcgagatg cccaacgatg aagaggcagc caaggcgatt gcggggctcg atggaaaagc
     1501 tgtcggcggc cgaaatttga aggttaatga ggctcgcccc aagggcgagt cgagcggtcc
     1561 tcggggcccg cgcagcggtg gtagcggtga ccggaatgat cgggggaatc gcggcagatt
     1621 ctccaatgag gattaccgcg aagccgcgcg acagccacgc gaacctcgct ggtagtgttg
     1681 agaattgtgc agttgcggta ctgtcgccat ccggcgaacg cccttttaag gagacatcag
     1741 tgccaactac tttccagaaa cgccaaaagg aaatgaagag gatggagaaa gcgcgcatga
     1801 aagctgaacg ccgcgctcag aaaaaactcg aacctcgaaa caatgaagag gatgaattga
     1861 aggttttaac cggacccgct cctttggaag acgatccgct ggcggaccca tcggattggg
     1921 gaccgtcagg cgcttagaaa ccgggcactt cccgcccgac gccgcatcca ctctaattta
     1981 tcgcagggat ttcagacact tcgcccaggg caagccgtcc tgcgccttcc gtcgcacgaa
     2041 cgaggaaatc gagtagcccg cgctactttt gaaaatgcgg catcacttca tccctgaata
     2101 gctcaagcga gcggacgctt tcctcgtgag tgagttcccc ataggcaaag cgcgcgacaa
     2161 aatagtttgc gccgctttgg tccaattgct cctcgatctt tgcgcggacg gttgcgggag
     2221 tgcccgcaat gagtgatgct tcgctggcgc gcaatacctc ttccaggctc atggccgtga
     2281 cgttgtattt ccgccagagg tagacgagac tgtcgtagaa cccctttgat ccaaacttac
     2341 cgcgctcctc ggcctgccgg tcgttttcgc ccacatagag gttgcgagcg atgcccgtct
     2401 tgggcatctt caggcctttt ccgccgtacg cggcgttcca tgaatccttg aatgcacaga
     2461 tcacggctcg ggcctcgggc gctcccagca agttcaccac gttcatattc tggcgcgcgg
     2521 cccgcgccgc actttccgga gcgttcgtgg cgtaccacag cggcgggtga ggcttttgca
     2581 gcggcttcat ctcaatgggg acattggtga attcgaaata tttgccatgg taatcgacca
     2641 cgtcctgtgt gagggcagcc atcaatactt gcatggcttc gccgtagata gccggggctt
     2701 cctgctcctt tacgttgaag aagcccaact cgacgccgga aaccccacga cccgtaccta
     2761 tgtcgaaccg gccgttggtc agatggtcga gcatgcagat ttcggcagcc agacgcagag
     2821 ggttgtaaag ggtggcgatc aacaccagcg gagcgaatcg gatattttta gtaatccgcg
     2881 aggccgcggc cagaaacacc attggggacg gggccagccc aagaggcgtg aaatggtgct
     2941 cggtgacatg gaatgtgctg aaaccggctt gatcatagag ttccatcagc ttcatgcggc
     3001 tgtcgtaggt ctcgtggatc ggcttcttct gcctgtccac atggtcgatg acgccaaaat
     3061 ccattttcat tcgtcggcct tcccggaaaa tgtgaggatt gctgcctcga cggagcagtt
     3121 ggagacgcac aaactgtacc gctgcacccg ccccatggca aattgcgagt ttttcgatcg
     3181 gaactagcac tctttgcttg acaaaatttc ggtatgcctt tactagtcag gatggctatt
     3241 catacgtaac gatgaaagag cattcgtcta atccggagcc tggtagtcgc cgccgcccca
     3301 ccaggcaagc agagggtttg cggcgcgcat cggctagttc caggctcgga ttcgagcagt
     3361 gttcctggat gccgtgtgaa ggcgatctgc ttcgggccga ttggtgttat gggaacaggg
     3421 aggcgaggcc gggtctgatt tgaacatggg cggttttttg cttgcccgtg gcccggtaga
     3481 tcgccggtcg cgggcttttt ttttgcctcg cggatagttt aaaggagctt ttatgctgca
     3541 cgcaacggaa gtcctgggcg ctcaaactta tgactcggcg ggcaatttcg tgggccgggt
     3601 gaaggaactt tttatcgtcc ccgccgagca gccaaatcga gtatcgcgtc tactgattgg
     3661 acgtggcaag taccgctcac tgattgccag atacgaccaa ataggcaccg tggagcccgg
     3721 ccgggtgaat ctgacagcgg atgagtccgc gctggaactt taccatccca atgaagcctg
     3781 gctggcgctt aggaaagacc tgctggacca gcaaatcatc gatacgaacg gtcgcaaagt
     3841 ggttcgcgta aacgatgtag atatggcgga ccaacgcacg aacggcaaca cggaactgcg
     3901 actcactcaa gtggatgtcg gcctgcaagg ggccgttcgg cggctgctcc aaggcttggt
     3961 gccgccgacc gggattcgcc gcctggacaa caacgtgcgc ggacgatcca tacggtggga
     4021 atttgtcaat ctaatcgagc ccgatcctct acggcgcgtg aagctgcggc tttcgagcga
     4081 caagctcgcc gagcttcatc cggcggacat cgccgacatc gtggaggaag tctctccggc
     4141 agagcgccag tccatcctcg attcgctcga cgaggaaact gcggcgggaa ccatcgcgga
     4201 attggacaag cgccttcaga cgcaggtggt cgagaagctc gatcccgaaa aggccgccga
     4261 tatcatcgag gaaatgaacc cggacgaagc cgccgacttg atccagaatc tcgaaccgga
     4321 aacgtccaag gaagttctcg aggaaatgga ccatcgcgaa gcgcgcgaag taacggagct
     4381 gatgcgcttc gatcagaata cagccggcgg aatgatgaac acggaaatcg tggtggttgg
     4441 cgaggacgcc actcgcggcg aagtggttga ctacatccgc ttccatgaaa tggcgctcga
     4501 tcagctcgat aacatcgtcc tgataaaccg cgatgcggtc ttggccggta cagttcccct
     4561 tgcccggctg atccttgcca gcggcgaaca gcgcatggcc gagctggcct tcgaaccgct
     4621 actctcagtg agaccagatg cgaaggacaa cgaagtcttc gagctttttg ataaatacaa
     4681 tctgcgcagc ctggcagtag tggatgagag caactgtccc ttgggcacga tcgccgtgga
     4741 cgatgtggtg agccgcctgc gcgcaaaaat ttagctgaat agaacgtccg gcgagcagcg
     4801 cgccaacaat cagccaagat tccataaacg aagcgcaatg aacacttggg ccacattcac
     4861 ggataccttt tcgcgcctgg ccggccgagc cggcaatttg aagcgtcgct cgcgcggggc
     4921 gcggaggcgg ctcgcactgt ttatggcggt ggtcggtcca ggtctcatca cctcaaacgt
     4981 agacaacgac gcgggcggaa tcgcggttta caccaccgcc ggcgcccatt ttggctacgc
     5041 gttgctctgg tcgctgatcc cgatgaccat cgctctctac gtttgcgaag agatgtgcgc
     5101 ccgcatgggc gtggtgacgg gcaaggggct ttcagattta atccgcgaag agtttggatt
     5161 ccgctccacg ttcttcgtga tgctggccgc gttcgccgtg gacctcagca acaccgtggc
     5221 ggagttcgcc ggtgtggcgg cttccatgca gatttttggc atcagcaaat acgtggccgt
     5281 gccaatcgcg gcgatcgccg tgtggattct cgttgtgagc ggcagctatc gccaggtgga
     5341 aaagatcttt ctggcagcct gcacattgta cctggcctac gttgtatccg cggtgctcgc
     5401 gaaaccgaac tggatctctg cggcaaaagg cgtggttccc tcagtgcaca tgaactcgt
//
LOCUS       DATNDT010000463         3997 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3901255, whole genome shotgun sequence.
ACCESSION   DATNDT010000463 DATNDT010000000
VERSION     DATNDT010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3997
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3901255"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VN788_15345"
     CDS             complement(<1..201)
                     /locus_tag="VN788_15345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21957.1"
                     /translation="MASRREFLQIGVAALALPISARGISPAIAELAGEPARAPLYKVV
                     FDERFPASRKFASEAKSLGVAVQ"
     gene            458..1126
                     /locus_tag="VN788_15350"
     CDS             458..1126
                     /locus_tag="VN788_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21958.1"
                     /translation="MRIIRSMAIAAAGLGSLACLGAMAQNNGPLQAPQQKGQAPAQLI
                     ATPRQDRHDGLSITVDPYNEPTRAKQKFGKANPLTAGILPIEVSMHNDTERPIHVDLS
                     TIQLEVHFSDDHHQELDWLPAQQVANQVAHPKGPAAPTARRFPIGVPSGADSKADKMA
                     DILKPLTLDADIVPPMGTIHGFLFFDLNHDMSLVQNASLYVPDVNTVPASKPMMFYEV
                     PLGE"
     gene            complement(1199..3082)
                     /locus_tag="VN788_15355"
     CDS             complement(1199..3082)
                     /locus_tag="VN788_15355"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU21959.1"
                     /translation="MNKGCCPFIALFVALLLAFALARATFAQDEPAQLPADAQTPRGA
                     APPSQGQPGAPARPPQQRPPGMFMAGPLPHGTVQNYKPVTDQDLLNPSPDDWLQYSRT
                     YDAWRYSPLKQINTKNVSKLHIAWVQGLPAGSTETIPIVHDGIIYTIAPGADVMAING
                     ANGDIIWEYKRTYKNPQVAAMERTKALAIYKDMIYYTAPDGYVVALDAATGKVKWETY
                     KTDTQNTSGAIVVDGKVISGGTCGKGRESCFIEADDADTGKKLWRFYNVAGPDDPGYS
                     SWGGADNTNFTASTWGLPGNYDPVRKVLYWGIANPSPYERLARHGGNPDGTSRTAPAD
                     LYSNCTVALDPATGKLQWYYQYLPADDWDTDHTHERTLVTTTFDPDPKAVKWFNSEVP
                     RGSKHDVVAIVGESGMMFVNDRNNGQFLWAEPIPVDAPNMAISNIDTKTGKTTINWDV
                     VMKKPGDHHVICFWNDRSYWPTAYDPVTNSIYTSYVDNCLDMTANGLSGFRKGILRPG
                     SDINAIAGIAKVNLSTGEMMKFGVQRAPGTGAILATAGGLIFHGDINRRFRAYDAATG
                     KQLWESILGGPISVSTVTYAINGKQYVMVITGDNLAEPVLSRQTETNLTRGHNAIYTF
                     ALE"
     gene            3770..>3997
                     /locus_tag="VN788_15360"
     CDS             3770..>3997
                     /locus_tag="VN788_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21960.1"
                     /translation="MSWLNQVGHLLQQYTSGGAAAAPAPDVHAHFDQVAQAAPSSVIA
                     EGLAAAFRSNQTPAFGQMLSTLFTNSNGDQKA"
BASE COUNT          799 a         1100 c         1217 g          881 t
ORIGIN      
        1 ttggacagca acgccgaggc tctttgcttc actggcgaat ttgcggctgg cggggaatct
       61 ttcgtcaaat acaactttgt aaagaggcgc gcgcgccggt tctccggcca gttcagcaat
      121 cgcgggcgaa atgccgcgcg ccgaaatcgg caacgccagc gcggcaaccc cgatttgcag
      181 aaattctctt cggctcgcca ttggatcggc tcctatttgg gagaggaccg caagcgggtg
      241 gaagtttgca cgttgaaatt gaagtgtcaa gcggaatctg ttttttgcgg caggcgcatt
      301 ggagaccgga ggatagtgcc gcctcaatta agggcaacgc taagcgccac gagagtggga
      361 agtggggtat agtgccgaca atggagggaa tcggcggcgc gcaacttccg ctgtgcggcg
      421 tgcatcggaa caagcaacga cgatgcgaac tacaatcatg cgaatcatcc gatcgatggc
      481 gatagccgcg gctgggctgg gatcgctcgc ttgccttggc gcgatggcgc agaataatgg
      541 gccgctgcag gcgccgcagc agaaggggca ggctcccgcg cagctcatcg cgactccgcg
      601 acaggatcgg cacgacgggc tgagcataac ggttgatcct tacaacgagc ctacgcgagc
      661 caagcaaaaa tttggcaagg ccaatccgtt gacggctgga attctgccca tcgaagtatc
      721 catgcacaac gacactgaac gcccgattca cgtggacttg agcacgatcc aactcgaagt
      781 gcatttttca gacgaccatc atcaggaact cgattggctt cccgctcagc aggtggcgaa
      841 ccaggtggcg catccgaaag ggcccgccgc accaaccgcg cgccgtttcc cgattggcgt
      901 gccttcgggc gcggattcca aagcggacaa aatggccgac atcctgaagc cgctaacact
      961 ggacgccgat atcgtcccgc cgatggggac gattcacggg ttcctatttt tcgatttgaa
     1021 ccacgatatg tcgctggtgc aaaacgcgag cctctacgtc ccggacgtga acacggtgcc
     1081 ggcgagtaag ccgatgatgt tttacgaagt acctctcggg gagtgacggc cccgcggcta
     1141 aagccgcatc atacggcggc gttttcggcg gggctgaagc cccgcccttc caccctgatt
     1201 attccagcgc gaaggtgtag attgcgttgt ggcctcgggt gagattggtt tcggtttgcc
     1261 tgctgaggac cggctcggcg agattgtctc cggtaatcac cattacgtac tgcttgccgt
     1321 ttatcgcgta cgtgacggtg ctcaccgaaa ttggtcctcc gagaatggat tcccagagct
     1381 gcttgccggt cgcggcatcg taggcgcgaa agcggcgatt gatgtctccg tggaagatca
     1441 aaccgccggc agtggcaagt atggcgccgg tgcctggcgc tcgctgaacg ccgaatttca
     1501 tcatctcgcc ggttgaaagg ttcaccttgg cgatgccggc aatggcgttg atatcagaac
     1561 ctgggcgcaa gatgcctttc cggaatccgc tcaaaccgtt cgcggtcatg tccaggcaat
     1621 tatcgacgta ggaagtgtaa atagaattcg tgactggatc gtaggccgtg ggccaatagc
     1681 tgcggtcatt ccaaaagcaa attacatgat gatcgccggg ctttttcatg accacgtccc
     1741 aattgatggt ggtcttgccg gttttggtat cgatattgga aatggccata ttcggcgcgt
     1801 caaccggtat cggctcggcc cagaggaatt ggccgttatt gcgatcgttc acaaacatca
     1861 ttccgctttc gcccacaatc gcgactacgt cgtgtttcga tccgcgcggc acctccgaat
     1921 tgaaccactt cacggccttg ggatcgggat caaacgtggt ggtcacaagc gtgcgctcgt
     1981 gggtatggtc cgtgtcccaa tcgtctgctg gaagatactg ataataccac tgaagtttgc
     2041 cggttgcggg atcgagagcg acggtgcagt tgctatagag atcggcgggc gctgttcgcg
     2101 aagtgccgtc aggatttccg ccgtgacgag cgagtcgctc gtaaggtgaa ggattcgcga
     2161 ttccccagta gagaactttg cggactgggt cgtagtttcc cggcaggccc caggtggagg
     2221 cggtaaaatt tgtgttgtcc gcgccgcccc atgaggagta gccagggtca tcgggtcccg
     2281 caacgttgta gaagcgccaa agctttttgc cggtgtcggc atcgtcggct tcgatgaagc
     2341 agctttcgcg gccttttccg caggtgccgc cagaaatcac tttgccgtcg accacaatcg
     2401 cgccggaagt attttgcgta tcggtcttgt aagtctccca cttgaccttg ccggtcgcgg
     2461 cgtcaagcgc gacgacgtag ccatcgggtg cggtgtagta aatcatgtcc ttgtagatgg
     2521 cgagggcctt ggtgcgctcc attgcggcga cctgcggatt tttgtaagtt cgcttgtatt
     2581 cccagatgat gtcgccattg gcaccgttga tcgccatcac gtccgcgccg ggagcgattg
     2641 tgtagatgat gccgtcatgc acgataggga ttgtttcggt agagcctgcg ggcaggcctt
     2701 gcacccaggc gatgtgaagt ttgctgacgt tcttggtatt gatctgtttc agcgggctat
     2761 agcgccaagc gtcgtaagtg cggctgtact gaagccagtc atcgggactc ggattcagaa
     2821 ggtcctggtc ggtgaccggc ttgtagtttt ggacggttcc gtggggcaat gggccggcca
     2881 taaacattcc cggcggcctc tgctgcgggg gccgcgcggg ggcgcccggc tggccttggg
     2941 atggcggcgc agctcctctg ggcgtttgag catcggccgg cagttgcgct ggctcgtctt
     3001 gcgcgaaagt ggcgcgcgcg agtgcgaacg ccagtaaaag tgctacgaac agtgctatga
     3061 acgggcagca gcctttattc attccgattt tcccccaacg aattcgagct accgatttct
     3121 gctttaatgt gacgcgtgcg aaataattgg cgtttccctt ttgtttgcgc cgcgggttcg
     3181 cgaccggccg cgcgtttata tcccaagcgc agccgtgtac cgcgtcgaat ctggtctttt
     3241 cagtcacgtt tccaaattag cagaggaggg gaagaggtca agcgcggata cctgtggact
     3301 tccgtggcca gcgcactatt ccctcacggc gacgacctcg accagaaaac ctcgaggcaa
     3361 atccaagaga gattcctcga ctccgtcccg cgcgcggaca gaacgcgcga aaaacaaggc
     3421 gcgggactcc gctcggaatg acagcactac gtgaccatct atagaccgag tgacgcgtcc
     3481 agctagcctg attccttcgc ggcattagct gattgctatt cggtgccgcc ggcgaggttt
     3541 ggcacggctg gaaccgtgcc tgacgggggg aatttgatat cgctttttcg tggaatagga
     3601 ttacactttg tcacccacat ccaccgttgg acccccagcc ggtctcggcg tcttgtgcaa
     3661 agtccaggcc ttttcaattc gtctaacaat gactctgggt agtagagggg cccgaacttt
     3721 tccgacctga gcgcggacat tttcggcact gcggaccaaa ggaggcacga tgagctggct
     3781 caatcaagtt gggcatttgt tgcagcaata cacgtccggc ggggctgcag cagcccccgc
     3841 cccggacgtt catgcacatt tcgatcaggt ggcgcaagcg gctcccagca gcgtgatcgc
     3901 cgagggcctg gcggcggcat ttcgttcgaa tcagacgccc gctttcgggc agatgctctc
     3961 tactttattc accaactcga atggtgatca gaaggcg
//
LOCUS       DATNDT010000464         5493 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3927043, whole genome shotgun sequence.
ACCESSION   DATNDT010000464 DATNDT010000000
VERSION     DATNDT010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5493
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3927043"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1166)
                     /gene="flgK"
                     /locus_tag="VN788_15365"
     CDS             complement(<1..1166)
                     /gene="flgK"
                     /locus_tag="VN788_15365"
                     /inference="COORDINATES: protein motif:HMM:TIGR02492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HXU21961.1"
                     /translation="MGNLLTSLFNSAGALQAFQNGLDTTQNNVSNSSTPGYAKQIPVM
                     EANAFDLSISLPGGVTAGPTQSSRNAFAERNVQDQQSALGLSQQRASDLNPVQSLFDL
                     SGTTGIDAALSGLFNSFSQLSVSPNDPSARQSVIDQAEQTAKAFQQSANGLLSTSSSV
                     SRETSGYVAQVNQIADQIGQINADRRSGASSPNDAGADAQVHSALEQLSQLINFTALQ
                     QPDGTVTIYAGGQMPLVIGTTVQHLQTASVSSQTQILDGQGHDITGEFTGGELAGALS
                     VENTTIPSYLNSLNTLAQGVADQVNTTLSQGVDLNGNAPTTNLFSYNAVTGAALTLAV
                     TGITGDQLAAALPGSPGGNDNALNLSALADAKTVNGFSFSSYYGQVGSQVGQDVS"
     gene            complement(1159..1536)
                     /locus_tag="VN788_15370"
     CDS             complement(1159..1536)
                     /locus_tag="VN788_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21962.1"
                     /translation="MIGPRIHLAIQRTGRLEALLIEASPQSMERYAVEAAGLVSLLNE
                     IQESVTQGEPPPMNREYSTVRADLEVLSWRLSRARTLLGGVSAALEHWAGIALDDDSA
                     TASYNAAGSAFGPARSGMVITHG"
     gene            complement(1542..1826)
                     /locus_tag="VN788_15375"
     CDS             complement(1542..1826)
                     /locus_tag="VN788_15375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21963.1"
                     /translation="MKIYDTAASGIPTTPASGPGSVQAGRSGQGGRVSSPQGDHVDLS
                     HLSDRLNVEPASDSGRSQRVSQLEGLVQSGGYQVNAGAVSAGLIHQNMRA"
     gene            complement(1901..2272)
                     /locus_tag="VN788_15380"
     CDS             complement(1901..2272)
                     /locus_tag="VN788_15380"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU21964.1"
                     /translation="MARTEQALEATRPHAATSSLHLTRVRERKGVSLEQIAANTKIST
                     RFLIAIEAEEFHRLPGGVFNTSYLRQYAAAIGYEEEELLAHYRETIQPEETLPEAGCQ
                     PAHSEGLLSRCFRVLASAPRP"
     gene            complement(2391..3554)
                     /locus_tag="VN788_15385"
     CDS             complement(2391..3554)
                     /locus_tag="VN788_15385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXU21965.1"
                     /translation="MTCLEGQGGLNSARGRGLSVLVGDDQSDVREAIRLLLKAAGHQA
                     EAVESPEALLCAARERRFDVILMDLNYARDTTSGEEGLDLLMGLRSAGVRTPVVVMTA
                     WSSVELAVAAMQRGASDFVQKPWDNFRLLTTLQNQAEKADKENDDNRRWQREMEIARN
                     VQRKLFPRNTVRLATIEYDAGCVPAREVSGDYYDYLPCGPGRLGFVLADVSGKGVAAA
                     LLMANLQASFRGEPEDMIRHPVELIEAVNSRFRESTPVEQYATVFFGYYDESSRRLKY
                     ANCGHPAPLLRRRQGDVEWLESTGTVLGMFQKWNGEEREVEIGAGDVLVAYSDGVSEA
                     ANAAGEELGRAGILFAARTESTPSQLVAALLDGPLAAGSGQADDRTILAIRGK"
     gene            complement(3690..4928)
                     /locus_tag="VN788_15390"
     CDS             complement(3690..4928)
                     /locus_tag="VN788_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HXU21966.1"
                     /translation="MARIFPFQPYRYSLKAGPIEKLVTQPYDKISPEMQTRYLAQNPN
                     NLVRVILGERLPSDNEQQNVYTRAAGYLTDWIASGILAQEAEPSLYAYFQEFSVPDTG
                     EKLNRKGFIGVGAVEDYSENVVHRHEQTLSGPKKDRMELLKHTHAHFGQIFMLYPDPK
                     GEIDQILDEAAKSAPLGKIVDEYGATHTFYKISDPARVAKIQQLMSDKKLLIADGHHR
                     YETALAFRKGNPNLPESDRAMMTFVNMNSNGLRILATHRLVNGLENFQASDLLASLDV
                     RKLAGIGDLKAIFTKPDLSKVRIGIVVRGLEGVYLMERARAPRELDVEVLHKEILAGK
                     LGIGEDAIREERFLKYARGIDPAFEAVQNGKAQVAFLLEPTTIEQVADIAFSGGVMPQ
                     KSTDFYPKLLSGLTIYRLPR"
     gene            complement(4947..>5493)
                     /locus_tag="VN788_15395"
     CDS             complement(4947..>5493)
                     /locus_tag="VN788_15395"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HXU21967.1"
                     /translation="SLDTLQAAGVYQTGLLLALVTAIFYMRKTGLPVLKTSDVFAPGV
                     AAGHAVGRLGCFSAGCCWGIECHLPWAVTFTNPDAHKLVGVPLGVPLHPTQLYEAFAE
                     TLICIFLLLRTRRAHAAGEILGWYLVLYSVARFLIEFFRNHEQATHLGLSLTQWISLG
                     TLALGIWLLRPKSPKLAVSHS"
BASE COUNT         1108 a         1667 c         1542 g         1176 t
ORIGIN      
        1 gagacatcct gcccgacctg agaccccact tgcccgtaat agcttgagaa gctgaaccca
       61 ttgactgtct tcgcatccgc caaagcagac agatttagcg cgttatcgtt gcctccagga
      121 gaccccggaa gcgcggcggc gagctgatcg ccggtaattc cggttaccgc caacgtaagc
      181 gccgccccgg tcaccgcatt gtagctgaac aagttcgtgg ttggcgcatt accgttgagg
      241 tccacgccct gtgagagcgt tgtatttact tggtccgcaa caccctgcgc aagcgtgttc
      301 aagctgttga ggtaagacgg aatcgtcgta ttctccacgc tgagcgcgcc ggcaagctcg
      361 ccgccggtga attcgccagt gatatcgtgg ccctggccgt cgagaatttg cgtctgcgaa
      421 gaaacagacg cggtttgcaa atgctggacg gtcgtaccga ttaccagcgg catttggcca
      481 ccggcgtaga tcgttaccgt gccgtccggc tgctggaggg ccgtaaagtt gatgagctga
      541 gagagctgct cgagcgcgct gtgcacttgt gcgtcggcgc ccgcgtcatt cggactgctg
      601 gcgccagatc gccgatcggc gttaatctgc ccgatctggt ccgcgatctg gttcacttgg
      661 gcaacatatc cggacgtttc ccgagagacc gaagaggaag tgctcagcaa accgttagcc
      721 gattgctgaa acgctttggc cgtctgctca gcctgatcga tcacggattg ccgcgcactt
      781 ggatcgttgg gactcactga aagctgtgaa aagctattga aaagcccgct aagcgctgca
      841 tcgatccccg tggtgccaga gagatcgaat agtgactgga ccggatttag atcggatgcc
      901 cgctgctgag aaaggccaag tgcggattgc tggtcctgaa cgttccgctc ggcaaaagcg
      961 ttgcgggaac tctgggtggg gccagccgta actccaccag gcaggctgat gctgaggtcg
     1021 aacgcattcg cctccataac ggggatctgc ttcgcgtacc cgggcgtgga ggaattgctt
     1081 acgttatttt gggttgtatc caatccgttc tggaacgcct ggagtgctcc ggcagaattg
     1141 aacagcgatg taagcaagtt acccatgtgt aatcaccatg ccggaacgcg cgggtccgaa
     1201 ggcggaaccg gcggcattgt agctcgctgt ggcgctgtcg tcgtcaagag caatcccggc
     1261 ccaatgctcc agcgcggccg acacgccgcc cagcaatgtt ctggcgcgcg ataaccgcca
     1321 actcaaaact tccagatccg ctcgaacggt gctgtattcg cggttcatgg gcggcggttc
     1381 cccttgggtg acggactcct ggatctcatt cagcagactc accaagccag ccgcttccac
     1441 ggcataccgt tccatcgatt gcggcgatgc ttcgatcagc aacgcctcta accttccggt
     1501 acgctgaatt gccagatgta ttcgcggccc gatcatcgcg tttacgcccg catgttttga
     1561 tgaataagcc cagcgctgac ggcgcccgcg ttgacctggt atccacctga ttgcaccaga
     1621 ccctccagtt gcgagaccct ctgcgagcgt cctgaatcgc tggcaggttc cacgttcagc
     1681 ctgtcactaa ggtgcgacag gtctacatga tctccctgcg gcgacgatac tcgcccgcct
     1741 tggccggagc gtcccgcctg caccgaaccc ggacccgacg ctggggttgt cgggattcca
     1801 cttgctgctg tgtcgtagat cttcatgaga aaaagggctt gccagcctcc tcactcactt
     1861 tatcggcggg cttgagcgga aactttagag agaaatttct ttagggacgg ggcgctgaag
     1921 cgagcacccg gaagcagcgt gaaagcagac cttcactgtg ggctggctga cagcccgctt
     1981 ccgggagagt ctcttcaggc tgaatcgttt cgcggtagtg ggcgagcagt tcttcttcct
     2041 catagccaat cgcggcggcg tactgacgca agtaactggt gttaaacacg cctcccggaa
     2101 ggcggtgaaa ctcctcggcc tcaatggcga tcagaaaccg cgtactgatt ttcgtgttcg
     2161 cggcaatctg ttccaaagaa acccccttac gctcacggac cctcgtcagg tgcagtgaac
     2221 tggtggcggc gtgaggccgc gtcgcttcga gtgcttgctc cgtgcgtgcc atgttgttgc
     2281 tcgatctata tttggctaag ctgacaggat atcgaattcc attcgaacat gaagtggcaa
     2341 aactgttcca gctctctcac ttacaaaatc gtacatccgg cgccgtccgc tcacttcccg
     2401 cggatcgcca gaatcgtccg gtcgtcggct tgcccggaac ccgcggctaa cggaccatcg
     2461 agcagcgcgg ccaccaattg ggacggtgtt gattccgtcc gcgccgcgaa cagaattccc
     2521 gcccttccca gctcttcgcc agcggcgttc gccgcctcgc tgacgccatc cgaataggcg
     2581 accagtacat cgccggctcc gatttccacc tcgcgttctt caccgttcca cttctgaaac
     2641 atccccagta ccgtaccagt cgattccagc cattctacat caccttgacg ccgccgcaac
     2701 aggggtgccg gatgcccgca attcgcgtac tttagcctgc ggctcgattc gtcatagtac
     2761 ccgaaaaata ctgtggcata ttgctccacg ggcgtcgatt cccgaaatct agaattcacg
     2821 gcctcgatca gttccacggg atgccggatc atgtcctccg gctcgccacg aaacgacgcc
     2881 tgcaaattcg ccatcaatag cgccgccgcc acgcccttcc cggaaacgtc ggcaagcaca
     2941 aagccgagcc ggccgggccc acagggcagg tagtcgtaat agtcgccgct tacctcccgt
     3001 gcgggtacgc agccagcgtc gtactcgatg gtcgcgagcc gcacggtatt tcgaggaaac
     3061 agcttccgct gcacattccg ggcgatctcc atctcccgct gccagcggcg attgtcatcg
     3121 ttttccttat cggccttttc cgcctgattc tgtagggtgg tgaggagacg aaaattatcc
     3181 cacggcttct gaacgaaatc cgaagcgccg cgctgcatcg cggcgaccgc caattcgacg
     3241 ctgctccagg cggtcatcac gactacaggc gtccgcacgc ccgcgcttcg aaggcccatg
     3301 agcagatcta gaccctcttc gccggacgtg gtatcccggg cgtagttcag gtccatcaag
     3361 atcacatcga agcggcgttc ccgagcggcg cacagcagag cttcgggcga ttcgaccgct
     3421 tcggcctgat gccccgcggc tttcagcaac agccggatgg cctcgcgaac gtcggactga
     3481 tcgtcaccga caagcacgct aaggccacgt cccctggcgc tatttaagcc gccctgccct
     3541 tccaaacaag tcatgaactt attcccggta gggcaagcgg cgggccatcc cggtaaaggt
     3601 gctaacccgt tcggggtcta tatcctctac cccgcaaggt atgctccatt acagccggtc
     3661 tgtccggttt tgggataact ctgttcggat taccgaggca aacggtagat agtcaggccg
     3721 ctaagcagct tggggtaaaa atcggtcgat ttctgcggca tcacgccgcc gctaaacgcg
     3781 atatcggcga cctgctcgat ggtggtcggt tccaggagaa aggccacctg cgcctttccg
     3841 ttctgtacgg cctcaaacgc cggatcgatt ccgcgagcat acttcaagaa gcgctcttcg
     3901 cggatggcgt cctcgccgat tccgagcttg cccgcgagaa tctctttgtg cagaacctcg
     3961 acgtcaagtt cccgaggtgc ccgggcgcgt tccatcaggt agacgccttc gaggccgcgc
     4021 accacaatcc caatccgcac cttactcaaa tcgggcttgg taaagatggc cttcagatcg
     4081 ccgatgccgg ccaactttcg aacgtccaaa ctcgccagca gatcggatgc ctgaaagttt
     4141 tcgagaccgt tcaccaaacg gtgcgtcgcc aggatgcgca gaccgttcga gttcatattg
     4201 acgaatgtca tcattgccct gtcgctttcc ggcaggtttg gattgccctt gcggaatgct
     4261 agcgcggttt cgtaacgatg atggccgtcg gcaataagca gtttcttgtc cgacataagt
     4321 tgctggattt tcgcgacgcg cgccggatcc gaaatcttgt agaacgtgtg ggtggcgccg
     4381 tactcgtcca cgattttccc aagcggcgcg cttttcgccg cttcgtccag gatttgatcg
     4441 atctccccct ttggatcggg gtagagcatg aaaatctgtc cgaaatgggc atgcgtgtgc
     4501 ttcagcagtt ccatgcgatc cttcttcggg ccggacaacg tctgctcgtg gcgatgcact
     4561 acgttctctg aatagtcctc gaccgcgccc acgccgatga agccctttcg gttcaacttt
     4621 tctcccgtgt ccggaacgga aaactcctgg aaataggcat agaggctggg ctcggcttct
     4681 tgtgctagga ttccactcgc gatccaatca gtcagatacc cggccgcccg cgtgtacaca
     4741 ttctgctgtt cgttgtcgga gggcaaccgc tcgcccagga tcacgcgcac cagattattc
     4801 gggttttgcg cgagataccg ggtctgcatc tccggagaaa tcttgtcgta aggctgggtt
     4861 accagctttt caatcgggcc agcttttaga ctgtaccgat acggttgaaa cggaaaaatt
     4921 cgagccatgg tgaaactttt aggatattac gagtggctga ccgccagctt cggcgacttt
     4981 ggccgcagca gccagattcc caaagctagt gttccgagcg agatccattg tgtcaacgaa
     5041 aggcccagat gcgtcgcctg ttcatggttg cggaagaact cgatcagaaa ccgcgccacc
     5101 gagtacagca ctaaatacca accgagaatt tccccggcgg cgtgcgcgcg gcgcgtgcgc
     5161 aacaaaagga atatgcaaat gagcgtttcg gcgaatgctt cgtaaagttg cgtgggatgc
     5221 agtggcacgc cgagaggaac gccgactagt ttgtgcgcat cgggattggt gaacgtgacc
     5281 gcccacggga gatggcattc gatcccccag cagcatccgg ccgaaaagca gcccagccgt
     5341 ccgacggcgt ggcccgcggc gactccggga gcaaacacgt cggaggtttt cagaaccggc
     5401 aggcccgttt ttcgcatgta aaaaattgcc gtgaccagtg ccagtagaag accggtctga
     5461 tacacgcccg ccgcctgcaa tgtgtcgagc gaa
//
LOCUS       DATNDT010000465         5453 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3907833, whole genome shotgun sequence.
ACCESSION   DATNDT010000465 DATNDT010000000
VERSION     DATNDT010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5453
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3907833"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VN788_15400"
     CDS             <1..469
                     /locus_tag="VN788_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU21968.1"
                     /translation="PILMLTARGDEADRVIGLELGADDYVTKPFSPRELVARVKALLR
                     RLEWPTDEARPVTVGLLRIDPLLYRAESNGKQLPLSTLEFRLLYYLASRPNRVFSRDQ
                     LLDAVWGSERFVTPRSVDVYVRRLREKMEKNPEEPQHLKTVRGAGYIFETKVN"
     gene            476..2269
                     /locus_tag="VN788_15405"
     CDS             476..2269
                     /locus_tag="VN788_15405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU21969.1"
                     /translation="MKLARSGVFWKLGLTYFVLLIAVLAAVDFYATRILRQNYVHAAD
                     ERLNSLALIARSNPPDFDSETALASWTGGMSRSGARVTIIASDGRVLADSAHDIETME
                     NHSNRPEFRQAMGSGQGESVRHSHTLDRDLVYWAVRFDRPSELPIVIRFATPLAQINS
                     ALAEVRRKLWEASLVILLLAIGISALFSRRFAKRVGQLKELSERVSVGDFRPLIQESA
                     APDELGDLARALNVTASRLSATISSLSDERNRSAAILRSMAEAIAVIDGGEQITFFND
                     AFASLWNLDSRNHAKGPAVSVIRHPDVLRLIRSALAGQEARGELSLGSAQPRYFSVTA
                     ATVFAIGDALARPENQESPAGAVVVLHEITEIRRLEQVRKDFVANVSHELRTPLTAIQ
                     GFAETLLNGGLEDQENNRRFIEIIRNHASRLARLTDDLLELSKIEAGKLPIDLQRVDL
                     TDLIVPVIELAQSVAVKKGVSLNFISPVGFPMFRADVGLLREVLGNLLDNAIQYTPPN
                     GSVGISAERNGSSVIFTVADTGIGIPQAEHNRIFERFYRVDAARSREAGGTGLGLAIA
                     KHIVEAHGGQIWVDSEIGSGSKFHFSIPLAG"
     gene            2372..3067
                     /gene="phoU"
                     /locus_tag="VN788_15410"
     CDS             2372..3067
                     /gene="phoU"
                     /locus_tag="VN788_15410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HXU21970.1"
                     /translation="MASSARSSSNFFSTALENHLIAMVRTVEGSVNRSFEALLSRNAE
                     IASGVFLTEPRINESEILVDEHAVRLLQRADLTQEDVRHIVSTLKINNDLERIGDLAV
                     NICQCVISLCQSPSMDTPPELASMVPPVRAMVAQCLGALIHRNVTLAAEVLEGEEAVD
                     RQRDRVVETLIGAVKKEPSLAAVNIQYILAARHMERMADHSTNIAEDVVFWLREVDIR
                     HNRNSPPIPSNLE"
     gene            3330..5039
                     /locus_tag="VN788_15415"
     CDS             3330..5039
                     /locus_tag="VN788_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21971.1"
                     /translation="MQRVSTALLAAVLFLAVPVMARAGGDKGAVPADKATDPSTSKSP
                     DKAATAQPNKTARPSPKTDLQQLQDSVQQQQEELAAQRKALDEQQKEIEALKGASVSQ
                     PAIATNAQSPAEPSGAASRAPAISPMPLALSAKPSSIAPALRRTESNSMSHASTRLAA
                     PPPQDNGQKLSPIALQDFKIGATFYGSFSHFTNTGLVPAFQDTPTVQLGPGNSGLNAF
                     EVNRAYINLFYTPNAHVTLRITPDIYREIDSTDGAIAAGNGAQTNGTDNGNYSFRLKY
                     AYVDFQKIFGDGALKDDRVTFGQTQNPLIDWEEGLSGYRYAYLVPWNYLSLSSTYSGA
                     KLHGPIKVNGKEYLDYDIGVFNTASFHAIETNDKKQAMGRLTWYPLGTTVDLTGFGLT
                     FFENYGYNTKLQSQVSTPLNRLAVLGHFQSHNKQYEIVGEYDLGRNALSTGNLFSGAA
                     PVSGGPFFLGGGSSDIGTVSAAVLAGNATRQQGFAFFGHAGLGHSPFSVWGFYQYFQP
                     NTKFPGTDPIDLSRTVGGISYHVEKHFDVAFGDENFHWIHPQGMLGAGDTNGIVIWTQ
                     FTY"
     gene            5199..>5453
                     /locus_tag="VN788_15420"
     CDS             5199..>5453
                     /locus_tag="VN788_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HXU21972.1"
                     /translation="MMRSEGLVRCSMALCILLMGMSLVSCSRNAGTTTGSQTVLINGA
                     GSTFGYPIYSRWADDFQKQHPNVQINYQSVGSGAGIRQLLA"
BASE COUNT         1268 a         1512 c         1452 g         1221 t
ORIGIN      
        1 accgatcctg atgctgactg cgcgcggcga tgaagcggat cgcgtcattg gactcgaact
       61 gggcgcggac gattacgtca ccaaaccgtt cagccctcgc gagcttgtgg cgcgtgtgaa
      121 agcgcttctt cggaggctgg agtggcccac cgacgaagcc cgaccggtta cggtcggatt
      181 actccgcatt gatccgcttt tgtaccgagc tgaaagcaac ggcaagcaac tacctctcag
      241 tacgttagaa tttcgattgt tgtattacct cgcttctcgg cccaatcgtg tcttttcgcg
      301 cgaccaacta cttgatgccg tgtggggtag cgagcggttt gtaacaccgc gcagcgtcga
      361 tgtgtacgtt cggcggttgc gagaaaagat ggaaaagaat cctgaggagc cacaacatct
      421 gaagacggtt cgcggcgcgg gctacatctt tgaaacgaaa gtgaactaag agcaattgaa
      481 attggctcgc agcggtgtct tttggaaact tgggctgact tattttgttc ttctgatcgc
      541 tgtactggcc gcagttgatt tctatgctac ccgaatcctt cgacaaaatt acgttcatgc
      601 cgcagacgag cggctaaatt ccttagcgct gatcgcgcga tctaatccgc ctgatttcga
      661 cagtgaaacc gcattggcat cctggactgg cgggatgtcg cgtagcgggg cgcgtgtgac
      721 gatcattgct tcagatggcc gcgtccttgc tgattctgcc cacgatattg aaacgatgga
      781 aaatcactcg aaccgtcccg aatttcgtca agccatgggt tccggccagg gcgaatccgt
      841 gcgacacagt catacattgg atcgtgattt ggtgtactgg gcagtgcggt ttgaccgtcc
      901 atccgagtta cccattgtaa ttcgtttcgc aactccgctt gcgcaaatca acagtgcgct
      961 agcggaggtc cgtcgaaaac tatgggaggc ttcgctggta attttgttgc tcgccatcgg
     1021 catttccgcg cttttctcgc ggcgctttgc gaaacgagtt ggacaactga aggaattgtc
     1081 agaacgagtt tccgtgggcg acttccgccc actaatccag gagtctgctg ctccagacga
     1141 attgggagat ttagctcgcg ctctcaacgt cacagcctcg cgcttaagcg caaccatcag
     1201 ctcgctttcc gatgaacgca atcgctctgc cgcaatactg cgcagcatgg ccgaagcaat
     1261 agccgtgata gacggtggcg agcagatcac gttttttaat gatgcgttcg ccagcttatg
     1321 gaatctcgac agtcgaaatc atgcaaaggg gcctgccgta tcggtcatcc ggcacccgga
     1381 cgttctgcga ttgattcgta gcgcgcttgc aggacaggag gcgcgcggag agctttcttt
     1441 ggggtccgcg caaccaagat acttttctgt gactgccgcc acagttttcg ccatcggcga
     1501 cgcgctggct cgtcccgaaa accaggagag tccagccggc gcggtggtcg tgcttcacga
     1561 aataactgag attcgccgcc tcgaacaagt tcgaaaggac ttcgtggcca atgtctcgca
     1621 cgagttacgc acgcccttga ccgcgattca aggctttgcc gaaacacttc tcaacggagg
     1681 tctcgaagac caagaaaaca accgacgttt tatcgagatc atccgaaatc acgcgagccg
     1741 tcttgcgcgc ttaaccgatg acctcctgga gctatccaaa atagaagcgg gcaaactgcc
     1801 aatagatctg cagagagttg acctgactga cctaatcgtc ccggtcatcg agttggctca
     1861 aagcgttgcg gtaaaaaaag gggtctctct gaacttcatt agtccggttg gttttccgat
     1921 gtttagggca gatgtgggat tgttacggga agttctgggc aatttgctcg acaacgcgat
     1981 acagtacact ccccctaatg gcagcgtcgg gatcagcgca gagcgtaacg gttcgtccgt
     2041 catctttacc gtggccgata caggcatcgg cattccgcaa gcggaacaca ataggatttt
     2101 cgaacgattt taccgggttg atgctgcgcg ttcacgagag gcaggcggca ccggcctcgg
     2161 tctggcgata gcgaagcata tcgtcgaagc tcatggcggg cagatttggg tcgatagcga
     2221 aattggtagt ggctcaaaat tccacttctc catccctctg gccggttagc agttccccaa
     2281 aaattcatca agccgccatc ctcccgcaac atcggtcagg atcttttgac agagcagccg
     2341 actcggctgc cgccgcactt agggagaaag catggcctcg tcagcccgca gttcatcgaa
     2401 ttttttcagt accgccctcg aaaaccatct cattgcaatg gtgcgaaccg ttgaaggctc
     2461 tgtaaaccgg tcgttcgaag cgctgctgtc gcggaatgct gagattgcga gcggagtctt
     2521 tctgactgag ccgagaatca atgaatcgga aatcctggta gatgaacacg cagttcggct
     2581 gctgcaaagg gccgacctga cgcaagagga tgtgcgccac atcgtctcga ctttgaagat
     2641 caacaatgat ttagaacgga tcggcgatct ggccgtcaat atctgccaat gcgtaatttc
     2701 actctgccaa tcgcccagta tggatacgcc gcccgaattg gcatcgatgg ttcctccggt
     2761 aagggcgatg gttgcccagt gcctgggagc tctcattcat cggaacgtta cccttgcggc
     2821 tgaagtcctc gaaggcgaag aagcggttga tcgtcagcgc gatagggtgg tggagaccct
     2881 gatcggggcc gtgaaaaagg aaccttcgct ggcagcggtc aatattcaat acatcctcgc
     2941 tgcccggcac atggagcgca tggccgatca ctccacgaac atcgccgaag acgttgtctt
     3001 ctggttgcga gaagtagaca tccggcataa tcgaaacagc ccgcctatcc catcaaacct
     3061 cgaataacgt aacgcaatat tcataaacct cttattttcc cttcaaactc ttctgtgaaa
     3121 ttgccctcga tccaccgagc agatcttgag ccgcacccca catgcttcgc ccgcgcgaag
     3181 gtctgctgca tcgcccgaag agtggctggc gtctcttcac aaatttcgac gagccgtgac
     3241 gcagagcttc cacttgtggg ccgaagtaat tttctgcaag tcgcgaatca tgcgccgagt
     3301 gcgcaaagga aactacaagg aggaagtgga tgcaaagagt tagtacggct ttgcttgcag
     3361 cagtgctttt cctggcggtt cccgtcatgg ccagagcagg aggagacaaa ggcgcagttc
     3421 cggcagacaa agcgacggat ccgtcgacat ccaaatcacc ggataaagcc gcgactgcac
     3481 aaccgaacaa gacggctagg ccctcgccga aaacggactt gcagcagttg caggactcag
     3541 tccaacagca gcaggaggag ttggcggcgc agcgtaaggc gctggacgag cagcagaaag
     3601 agatcgaggc gctcaagggc gcatcggtga gccagccggc catcgcgaca aatgctcagt
     3661 ctccggcgga gccatctggg gcagcttctc gcgccccggc gatttcaccg atgccgctcg
     3721 cactttcggc gaaaccctct tccattgctc ccgctcttcg ccggacggaa tcgaactcaa
     3781 tgagccacgc tagcacgcgc ttggcggcac cgccgcctca agataacggt caaaagctca
     3841 gccccatcgc tcttcaggat ttcaaaatag gcgctacttt ttacggctcc ttctcgcact
     3901 ttaccaatac cggcttggtt ccagcatttc aagatacgcc tacggtccag ctcggacctg
     3961 gaaatagcgg acttaatgct ttcgaggtga accgcgccta tatcaatttg ttctacacac
     4021 cgaatgccca cgtaactctc cgcattactc ctgacatcta tcgcgaaatt gacagtactg
     4081 acggggccat cgccgctgga aacggcgcgc aaaccaacgg caccgacaac ggcaactact
     4141 cgtttcgcct caagtacgcc tacgtcgatt tccaaaagat cttcggagac ggcgctctga
     4201 aggacgatag ggtcacattc ggtcaaactc aaaacccgct tatcgattgg gaagaggggc
     4261 ttagcggcta tcgttatgcg tacctagtgc cctggaacta tctgagcttg agctccacct
     4321 actccggcgc caagctccat ggaccgatta aggtcaacgg aaaggagtac ctagactacg
     4381 atataggggt cttcaatact gccagtttcc atgcgattga aacgaacgac aaaaaacagg
     4441 ccatgggtcg cctgacctgg tatccgctgg ggaccactgt cgacctcacg ggttttggct
     4501 taaccttctt cgagaactac ggctacaata ccaaattaca gtcgcaagtc agcacgccgc
     4561 tgaatcgtct cgcagtcctc ggtcattttc aatctcacaa caagcagtat gaaatcgtag
     4621 gcgaatacga cctgggccgc aatgctctaa gtaccggaaa cctcttctcc ggcgcggctc
     4681 ccgtttcagg agggccgttt ttccttggcg gcgggtccag tgacatcggc accgtctcgg
     4741 ccgctgttct cgcgggaaat gccacgcggc aacaaggctt cgctttcttc ggccacgccg
     4801 gtctcggaca ttcacccttt agtgtctggg gcttctatca atatttccag ccgaacacga
     4861 aattcccggg tacggatccg atcgacttgt ctcgcaccgt cggtggcatc agttatcacg
     4921 tcgaaaaaca cttcgatgtg gccttcggcg acgaaaactt ccactggatt catccacagg
     4981 ggatgctcgg cgcgggcgat acaaacggga tcgtgatctg gacgcagttc acttattaag
     5041 cagaacttaa taccagacct gctagaggca agcggcacac agctcgaaat ggaaccgcca
     5101 atttgcaccg agatccgccg taatccggcg tgtcaccaag ctttaagaca gcagtaacag
     5161 cagtgtaaca atgcaattcc aaaattcttg ggggatagat gatgagaagc gaaggattgg
     5221 tccgttgcag catggctctg tgcatcctgc ttatgggaat gtctttggtg tcttgctcgc
     5281 gtaacgcggg cacaacaaca ggttcgcaaa cagttctcat caatggcgct ggttctacgt
     5341 ttggttatcc gatttactct cgctgggcgg atgatttcca aaaacagcat ccaaacgtcc
     5401 aaatcaatta tcaatccgtt ggctctgggg cgggtataag acagcttctg gcc
//
LOCUS       DATNDT010000466         4843 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2133268, whole genome shotgun sequence.
ACCESSION   DATNDT010000466 DATNDT010000000
VERSION     DATNDT010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2133268"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="VN788_15425"
     CDS             complement(<1..380)
                     /locus_tag="VN788_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016746190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21973.1"
                     /translation="MHVVSIRASFVPGGAARFVGRIQRRSILGILVALGIGLTCAAGE
                     KAPNSYRGWAVYGGGPDNIHYSSLTQINRRNAGQLQLAWSFDSHDAYPDSEMECNPIV
                     AGDTLYATTPRLRVVALDAENGRLR"
     gene            complement(1367..2374)
                     /locus_tag="VN788_15430"
     CDS             complement(1367..2374)
                     /locus_tag="VN788_15430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012239385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21974.1"
                     /translation="MPWYSANRDLPGTPYARIHLYKSLEELRDRFTTLVRDASAVIVG
                     SYVPEGVAVSEWVTDTAHGVTAFYDIDTPITLRKLEWGDYEYLSPDVIPKFSLYLSFT
                     GGPVLRRIRRQYGAGRTATLYCSADPELYYPETIEKRWDMGFLGTYGADRQPALDELF
                     IKPAMALRFGRFVAAGPLYPDTIVWPPNVQRIEHLPPASHRNFYNSQRFALNVTRADM
                     VQAGFSPSVRLFEAASCAVPVISDDWRGLHSIFRIGSEILVARNSAEILHHLKTVPEE
                     EQIRIGERARARVLRQHTPAHRAAEVERQVREILGTQTSRATTVSLYTRRDAICTTPE
                     A"
     gene            complement(2536..3630)
                     /locus_tag="VN788_15435"
     CDS             complement(2536..3630)
                     /locus_tag="VN788_15435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21975.1"
                     /translation="MRIAFFGSSLVSAYWNGAATYYRGIIHALYDLGHEITFYEPDAF
                     DRQQHRDIDDPPWAAVHVYSNCIDSVMTALEEARNSDLLIKASGVGVFDNFLERELPN
                     AARNDALKIFWDVDAPATLDRMNMNVLDPLREVLPRYDLVLTYGGGWPVISAYRRMGA
                     RDCVPIYNALDPATHFPVPPDPRYQGDVAFLGNRLPDREARVDEFFFKPANMRPKRQF
                     ILGGNGWQNRPMPPNVNYVGHTYTADHNAFNSTPMAVLNIHRDSMASYGFSPATRVFE
                     AAGAAACLISDAFQGVEEFLEPDSEILIAENSDGVLAHIDSLTPDRARRIGRAARERI
                     IARHTYRHRAAQLNALLGVAHSAAAVTSLA"
     gene            complement(3638..4768)
                     /locus_tag="VN788_15440"
     CDS             complement(3638..4768)
                     /locus_tag="VN788_15440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU21976.1"
                     /translation="MKVALFYHSLYSDWNHGNAHFLRGIAAELLSRNIELAIYEPFDA
                     WSLQHLITEQGEGVIEEFSRAYPNLRSVRYKFDEFDLATALDGVDLVIAHEWNAPELI
                     RSLGDLRGCSGDFRLLFHDTHHRSITAPAELASYDLSNYDGVLAYGDIVRQIYMARGW
                     AKRAWTWHEAADMRTFFPMPEIKKRGDLVWIGNWGDNERTAELNAFLIEPVKDLGLDA
                     SVFGVRYPVEAEAKLIQAGIHYGGWLPNFKVPETLAQFRVTVHIPRAAYARVLHGIPT
                     IRPFEALACGIPLISAPWKDTEGLFEPGEDFLVARDSEEMKRYLRAVLEDRDLAGRLA
                     RNGRNRVLRHHTCAHRVDELLAIYRELKEHPALMASNASAAD"
BASE COUNT         1127 a         1372 c         1290 g         1054 t
ORIGIN      
        1 cgcagtctgc cattttcagc gtccaaggca acgactcgca gccgcggagt cgtggcatag
       61 agagtgtcac cggccacaat ggggttacac tccatctcgg aatccgggta tgcgtcgtgg
      121 ctatcgaagc tccaagcgag ctggagttgg cctgcattcc gccgattgat ctgggtgagc
      181 gacgaataat gaatgttgtc ggggccgccg ccataaaccg cccagcctcg ataggaattc
      241 ggcgcttttt cgccggccgc gcaagtaagg ccgatgccta aagcgaccag gattcccaaa
      301 atcgagcgcc tttgaatacg gccgacgaat cgggcggcgc ctcccggcac aaaactggcg
      361 cgaatcgata ctacgtgcat gtcccttttt ccgcgggccg atcggacggg aacaaccgcc
      421 tcgatgtcag gcccacatta aaaatactca ggcggcgcgc cgccagcggt cgaacatttt
      481 taattcgtgt gacaaatctc cgccattata ctcacactga tcgttggcgg tgccaggcat
      541 cctctcgcgc aactcgagta caccctcggc ttctttgacc aattaacgcc aagtcgccaa
      601 atgtggtcgc cgccggcttt atcttgacgc attattgacg atcacgtctc gattcttgac
      661 tccgtcttga cgcgctggtt ttatagtcgg ttatggcagg gtggcgaaat gagcgcggcg
      721 gtacagcaac ggctaactca catttgggaa actcccgaca ccttcctcgg ctggtggtac
      781 cgtcgatcat caatagcgaa gatggtctga tccgcaaagt cttcgagcag ccgccaagtg
      841 ccgcgagaga tccgtcgagg ccgtctggtt ttgaatccga ttgctgcaat gatgccaccg
      901 gccgtccgta acggcaaagg agcaggagct tgcttcggaa atatgtggca tcccgtgccc
      961 gcgccgagtt gtgatgcacg gcgaaatcga cggttgccaa atcagcggac tccagcggaa
     1021 aatcggaact acagacacaa ggcagcccgg atcgtcttcg gctataacga ccccaacggc
     1081 accttgaaaa gcaaaagaac ttggcgttcc aaaacatcta gatttcaggc agattttgag
     1141 ccttcgcaag cgcttgcgaa cgcgatttgc gcgcaccgcg gaacgaccgg cgagcaccgc
     1201 tccgaaagct cccaaacaga tcgcgattac gcgtgtgggc cgcattgctt cagtgtgtca
     1261 aaaagcgaaa atttcagcgc ataattttaa ctcgtggcca cgccgtcgcg gattggagag
     1321 tagcgaccgg tcatccacct ttaaacttgc cgcggttcca agctgtttat gcttcaggtg
     1381 tcgtacagat cgcatcgcga cgggtataca aagatacagt cgtggcgcgg gatgtctgag
     1441 tccccaaaat ttctctcacc tgacgctcca cttcggctgc acgatgggcc ggtgtatgct
     1501 ggcgcagtac tctggcgcga gctctctctc cgatcctaat ctgctcctct tcaggtacgg
     1561 tcttcaaatg atgcaagatc tcggcactgt tccgcgcgac tagaatttcg gatccgatcc
     1621 ggaatatact atgcagaccc cgccagtcat cgctgatgac cggtacggcg catgatgcgg
     1681 cttcaaacaa acgcacgcta ggagagaatc ccgcttggac catatccgcg cgagtgacat
     1741 ttaaagcaaa tcgctgtgag ttatagaagt tccgatggct ggctggcgga agatgctcaa
     1801 tccgctgcac gttaggcggc cacacgattg tatccggata cagcggcccg gcggctacga
     1861 agcgtccaaa tcgaagggcc atggcaggct taataaacaa ctcgtccaaa gcaggctgcc
     1921 ggtccgcccc ataggtgccc aggaaaccca tatcccagcg cttttcgata gtttccggat
     1981 aatagagctc cgggtcggcc gaacaataca gagtcgctgt gcgtcccgcg ccatattgcc
     2041 gtctaatccg gcgaagcact ggaccgccgg taaaggacag atacaacgag aatttcggaa
     2101 ttacatcggg gctgagatat tcgtaatccc cccactccag cttacgaaga gtaattggag
     2161 tatcgatatc gtagaatgca gtaacaccat gagctgtgtc cgtcacccac tcgctcaccg
     2221 cgaccccttc cggaacatac gatcctacga ttaccgcgct tgcatcacgg acaagggtgg
     2281 tgaaccggtc ccgcagttcc tcgaggcttt tatatagatg aatcctggcg tagggcgtgc
     2341 cgggcaggtc gcggttggcg gaataccacg gcacatcgcg ctccagaaag cttacttcgt
     2401 gtcctcgttt tgtaagctcc cgagttagtc cacgatatgt tgtcgcgtgg ccgttccccc
     2461 aggacgatgt tatggacaat cccagaaaga caaaccgcag tttttctcct ctggcggagg
     2521 ttgaatagtc tgatatcatg cgagactcgt cacggcggca gccgaatgcg caacacctag
     2581 aagggcgttg agttgagcgg ctcgatgccg ataagtgtgc cgcgcaataa tcctctcccg
     2641 tgcggccctt ccaattctac gggcccggtc gggcgtcagc gagtcaatat gcgccaagac
     2701 tccatcgcta ttttcggcga ttagtatttc gctatcaggt tccagaaatt cctccacgcc
     2761 ttgaaacgcg tcgctaatta gacaagcggc agcgcccgct gcctcgaata cccgcgtcgc
     2821 gggcgagaaa ccgtagctag ccatactatc gcgatgaata tttagcacag ccattggtgt
     2881 cgagttgaat gcattatggt ccgcagtgta agtgtggccc acgtaattga cattgggtgg
     2941 catggggcgg ttttgccagc catttccgcc tagtatgaat tgacgctttg gccgcatatt
     3001 ggcaggcttg aaaaaaaatt catcaacacg ggcctcgcgg tccggcaggc gatttccaag
     3061 aaacgccaca tcgccttgat aacgtggatc gggcggcacc gggaaatggg ttgctggatc
     3121 aagcgcattg taaataggta cacaatcgcg cgcgcccatc cggcggtaag cgcttatcac
     3181 gggccagcct cctccatacg tcagaacgag atcgtatcgt ggcagcactt cacgaagcgg
     3241 gtccagcaca ttcatattca ttcggtcgag cgtcgccggg gcatcaacat cccaaaagat
     3301 ctttaaagcg tcattgcgag ccgcattggg aagttctctt tccaaaaagt tgtcaaaaac
     3361 tccgaccccg ctggctttaa tcagcaggtc tgaattcctc gcttcctcta gcgctgtcat
     3421 caccgagtcg atgcagttgg aataaacatg taccgctgcc cagggcggat catctatgtc
     3481 acggtgttgc tggcgatcga aggcgtccgg ctcataaaac gtaatctcgt gtccgagatc
     3541 gtataaggca tgaataatcc cgcgatagta tgttgccgca ccgttccaat atgcggaaac
     3601 caggctcgat ccgaaaaaag cgatacgcat aagggtccta atccgccgcc gaggcattgg
     3661 aagccattaa tgctggatgc tccttcaatt cgcggtaaat tgcgagtaac tcgtccaccc
     3721 gatgagcgca agtatgatgc cgtaaaacgc ggttgcggcc gttccgcgca agccgaccgg
     3781 ccaagtcccg atcctctaag accgcacgga ggtaccgttt catttcttcc gaatcgcggg
     3841 cgacaagaaa atcttcgcct ggctcgaata gcccctcagt atctttccag ggcgccgaga
     3901 tcaacgggat gccacatgct agggcctcga acggacggat agttggaatt ccatgcaata
     3961 ctcgcgcata agcggcgcgt gggatatgga ccgttacgcg aaattgcgcg agtgtttccg
     4021 gcactttgaa attaggaagc cagccgccgt agtgaatgcc agcctggatt agcttggctt
     4081 ctgcctcaac cggatagcgt acgccgaaaa ctgacgcatc taagccgaga tctttcacgg
     4141 gctcaattag gaatgcattc aattccgccg ttctctcgtt gtcaccccaa tttccaatcc
     4201 aaacgaggtc gccccgcttc ttaatttcgg gcatcggaaa gaacgttcgc atatccgccg
     4261 cctcatgcca ggtccaggca cgcttcgccc agccgcgcgc catatagatc tggcggacaa
     4321 tatcgccata cgcgagtact ccgtcataat tcgacaaatc ataagatgcg agttcggcag
     4381 gggcggtgat tgagcggtga tgcgtatcgt ggaaaagcaa tcggaaatcg ccgctgcacc
     4441 ccctaaggtc gccaagagat cggatcagct ccggcgcatt ccactcgtgc gcaatcacaa
     4501 ggtccactcc gtcaagcgct gtcgccaaat cgaactcatc gaacttgtaa cggacgctac
     4561 gaaggttggg atacgctcgc gagaactctt cgattacacc ttccccttgt tctgttatca
     4621 ggtgttgtaa agaccaggca tcgaacggct catagatagc gagttcaata tttcgcgaga
     4681 gaagttccgc ggcgatacct cgcaggaaat gcgcgttgcc atgattccaa tccgaataca
     4741 gcgaatggta gaaaagtgcg accttcatgc tttcgtcccg ccagccgcga cagcgtgctt
     4801 ggattcgtgg tgatgcatgg cccgcttgta ggcgtcgaga tac
//
LOCUS       DATNDT010000467         5531 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3293783, whole genome shotgun sequence.
ACCESSION   DATNDT010000467 DATNDT010000000
VERSION     DATNDT010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3293783"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VN788_15445"
     CDS             <1..1131
                     /locus_tag="VN788_15445"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU21977.1"
                     /translation="EEHLSALLQIQLLRQTEAQEGRAQELLNSAVSSQDTLYKTSAYF
                     SALSNLTIVSGIAGILACGSSMVFHGRLTIGTLIAFYSLLVQLFDPLSTAMEMYARAQ
                     RTFSSIRQIQCVLEISPGVMEHRNARALASDSAWDIAFQDVSFRYRQRSEVIRIPKLE
                     IRAGERIAIVGPNGAGKSTLGKLLARLYDLQSGAILIGGRDIRAVTLASLRSSVCYLA
                     AQPVLFHRSIAENLRIERSESSGDDLERVLRLVALTKYLDGCPGSLEECIQPGASNLS
                     NGERQRVAIARALLHRPRILILDETTSSLDPGSEETILRAIDRALPDSTLLVVSHRLS
                     SISWMGRILLMRQGQIVADGNHSVLKDASDFYAEFLASAALR"
     gene            1351..1626
                     /locus_tag="VN788_15450"
     CDS             1351..1626
                     /locus_tag="VN788_15450"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HXU21978.1"
                     /translation="MFTLADSIRRTQTEDGGILLDIHHGQMFSLNAVGSQMIELLEKG
                     LDEGQIAAHVARTYAADMETIRADVRDFIAVLQKHHILQVRSAGAVT"
     gene            1641..3044
                     /locus_tag="VN788_15455"
     CDS             1641..3044
                     /locus_tag="VN788_15455"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HXU21979.1"
                     /translation="MNSGETSTLQTYRLIITRRSAAELLVVPRGSGWAVPEVAVRPDQ
                     RLAEQLTARTRESRQLETYCLFVPGQANTREAAPETGYAVMESLLQNGEAPADTFWML
                     SQVALRKGTLESHERAIVQSSLEEMERYTAEPNRGPFGRPDWVKELFQWIQEHIGPLG
                     LRVTGAFNQLNGSTAFSLMRIETSGPAVWFKATGEPNRHELPVSVTLARLFPRFVPAL
                     LGVHSAWNGWLSREAPGATLDNFGDVSAWARTARTLAELQIASVGKSDDLLDSGCKDL
                     RMQRLIGEIDLFVASMSESMAMQKIEPPAVLTNSELRILGEELKEACSALEGFGLPDT
                     LGHIDFNPGNILVSPEACFFLDWAEACVANPFITFAYLREHSERQHLEGAGAVETMTA
                     AYLEPWCAFFSSRDLERGMAFSSLVAVFAYAVSGNAWRSREASRNPALAPYLRSLTRR
                     MHRDAAGIRGRSEPCLR"
     gene            3032..5068
                     /locus_tag="VN788_15460"
     CDS             3032..5068
                     /locus_tag="VN788_15460"
                     /inference="COORDINATES: protein motif:HMM:NF014896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HXU21980.1"
                     /translation="MPALTLPRPHAPIEPVTEILHGVAITDPYRWLEDRNSPRTREWI
                     EQQTRYARAYLDNVPGREVIRKRIREFLAVATYDSLQKAENRYFFRKRLPDEEQPGIY
                     MREGATGEDQLLVDPAAWGTGKYTAVKPLRVSGDGRLLLYEIKQGGERTGRFAIFDVV
                     ARQTLPDVLPRGYLRGFAFAPDGKSFYYVHEALDAERPFYRAAYQHTPGTPFSEDREI
                     FCAGEDANLRLCLIGDEERLGFLVYRFGEKTRTDFYLRSFEACGAPQVIVADARYSLG
                     PLLTSGKIFAITDRNAPNLRIVELRLQKNKEPLWVEIVPESDARIHQWAVARDRIFVS
                     YVRGTAFDVSILDFAGEPVGKMPVQSGETIRLMGGSAKSDELLVEAESFTEPITIFRL
                     PVNGGQRAPWAKRSIPFDASRYEHTQVWYTAKDGTRIPMFVLGRKDVLAAGSHPTILT
                     SYGGYGVSMTPQFSVFVAFLVERGCLFALPNIRGGSEFGAAWHIAAKRHNRQTAYDDF
                     LCAAEWLIASGRSAPGKLAIFGGSNSGLLVAAAMTQRPDLFRAVVCMVPMLDMLRYHL
                     FDNAHVWREEFGTAEDPDDFAVLASYSPYHRVQDGTAYPATMIVSGDADRNCNPLHAR
                     KMAARLQAANASGHPVLLDYSPFRGHSPVLPLSERIESLTDRMAFLCDQLGLSV"
     gene            5081..5455
                     /locus_tag="VN788_15465"
     CDS             5081..5455
                     /locus_tag="VN788_15465"
                     /inference="COORDINATES: protein motif:HMM:NF024862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HXU21981.1"
                     /translation="MRFLSLKAYGKLILFDLYLARGNFAALYGKVRNCRVRSQEPIHD
                     AAGRICAAMDMACIWYWKEARCLQRSAATACLLKQYGVRAQMVIGAQQLPFKAHAWVE
                     VDGRVVNDKPYMREMYAVLDRC"
BASE COUNT         1081 a         1653 c         1680 g         1117 t
ORIGIN      
        1 gaagagcatc tctcggcttt gctgcagatc cagctattgc ggcaaaccga agcgcaggag
       61 ggcagagctc aagaacttct gaacagcgcc gtttcgtcgc aagacactct ctacaagacc
      121 agcgcttatt tttcggcttt gtcgaacctg accatcgtgt ccggaatcgc cgggattttg
      181 gcctgcggaa gctcgatggt tttccatgga cggctgacga tcgggacttt gatcgcgttc
      241 tacagcctgt tggtgcagct cttcgatcct ctgagcacgg cgatggagat gtacgcaaga
      301 gcgcagcgca ctttttcgag catccgtcaa attcagtgcg tcctcgaaat cagcccggga
      361 gttatggagc accgtaacgc ccgggcgctc gcttccgatt ccgcatggga catcgccttc
      421 caggacgttt cgttccgcta ccggcagcgc agcgaggtca ttcggattcc gaagcttgaa
      481 attcgcgcgg gcgagcggat cgccatcgtg ggtccgaacg gcgcgggaaa aagcacgctc
      541 ggcaaactcc tggcgcggct ctacgacctt caatcgggcg cgatcttgat cggcggcaga
      601 gacattcgcg cggtgacgct ggcgagcctg cgatcatcgg tttgctatct tgcggcccag
      661 ccggttctct ttcaccgttc gattgccgag aacctgcgca ttgaaagatc cgaatcgagc
      721 ggggacgacc tggagcgcgt cctgcggctc gtggcgctga caaaatatct ggacggctgc
      781 cccggttcac tcgaagagtg catacagccc ggcgcttcca acctctcgaa cggggagcgc
      841 cagcgcgtcg ctattgcgag ggcgctgctt catcgcccgc gcatcctgat tctcgacgag
      901 accacttcgt ccctggaccc gggctcggaa gagaccattc ttcgggccat tgaccgcgcg
      961 cttcccgact cgacactctt ggttgtgtcg catcgcttgt cctcgatctc atggatggga
     1021 aggattcttt tgatgcggca agggcaaatc gtggcggacg gcaaccactc ggtgctcaag
     1081 gacgcgagcg atttctacgc agagttcctg gcctcggccg cgctgcgtta gtttgtgatt
     1141 ctgccgctct ccggtcggga acgcgaacac aggcgcacgt gggttttgca ggccacgctt
     1201 tgcatcgcct gtcttcccag cacgttgcaa taggacgttt ggttccgcga acagccgggg
     1261 cactttctgg aatggctttt cgaagcgtgc cacagtgctc gcatacgtga agccgccagc
     1321 tcacttccgc ccctgcgagg ttttgcccga atgttcacac tagccgacag catccgacga
     1381 acgcagaccg aggatggcgg gatcctgctc gacatccacc acggacagat gttttcgctg
     1441 aatgcggtgg gatcccagat gatcgagctg cttgaaaagg gtctcgacga aggacaaatc
     1501 gccgctcacg tggcccgcac gtacgctgcg gacatggaaa ccattcgtgc cgacgtgcgc
     1561 gatttcattg ccgtgctgca gaaacatcac atcttgcaag tgcgtagcgc aggagccgtc
     1621 acctagggca tgagtgattc atgaattccg gcgaaacatc gacgctccaa acctaccgcc
     1681 tgatcatcac gcgccgaagc gcggcggaac tgctcgttgt cccgcgggga tccggatggg
     1741 cggttcccga ggttgcggtt cgcccggacc aaagactcgc ggagcaattg acggcgcgca
     1801 cacgcgagag caggcagctc gaaacatact gcctgttcgt tcctggacag gcgaacactc
     1861 gtgaggcagc gcccgaaaca gggtatgccg tcatggaatc gttgctgcaa aacggcgaag
     1921 cgccggcgga cacgttctgg atgctctccc aagtggcgct ccgaaagggc actctcgaaa
     1981 gtcacgagcg cgcaatcgtt caaagctcgc tcgaagaaat ggaacggtac accgcggaac
     2041 cgaatcgtgg gccattcggc agacctgact gggtcaaaga actgttccag tggattcagg
     2101 agcacatcgg cccgctggga cttcgggtga caggcgcttt caaccagttg aacggaagta
     2161 ctgcgttcag cctcatgcga atcgaaacca gcggacctgc ggtgtggttc aaagcgaccg
     2221 gagaaccgaa tcgccacgag cttcccgtga gtgtgactct cgcccgcctt tttccccgat
     2281 tcgtacccgc gctcctcggc gttcactccg cctggaatgg gtggctgtcg cgggaggcac
     2341 ccggagcgac actcgacaac ttcggcgacg tttccgcttg ggcccggact gcccggacgc
     2401 ttgcggaact gcagattgct tctgtgggga agagtgacga tctgctggac agtggatgca
     2461 aagacttgag gatgcagcgg ctcatcgggg agatagatct cttcgttgcc tccatgagcg
     2521 agtcgatggc aatgcaaaag atagagcctc ccgctgttct cacgaattcc gaactcagga
     2581 ttcttggcga ggagttgaag gaagcctgtt cggcgctcga gggttttgga ttgcccgata
     2641 ctcttggcca catcgatttc aacccgggaa atattcttgt ttctccggag gcgtgcttct
     2701 ttctcgactg ggccgaggcg tgtgtggcaa atccgttcat cacttttgcc taccttcgcg
     2761 aacactccga gcggcagcat cttgaaggtg cgggagccgt agaaaccatg accgcagcgt
     2821 acctcgaacc ctggtgtgct tttttttctt cgcgcgacct cgagcgcgga atggcttttt
     2881 cgtccctggt cgcggttttt gcgtatgcgg tcagcggcaa cgcctggcgt tcccgggagg
     2941 cttcgagaaa tccggcactc gctccctatc ttcgcagcct cacacgtcgg atgcatcgcg
     3001 acgccgccgg catccgggga aggagcgaac catgcctgcg ctgacgctcc cgcgtcccca
     3061 cgcgccgatc gaaccagtca ccgagattct tcatggtgtc gcgatcaccg atccttaccg
     3121 ctggctcgaa gaccggaact ctccccgcac gcgcgagtgg atcgaacagc aaacccgcta
     3181 cgctcgcgcc tatctcgaca acgttccggg gcgagaggtc attcggaaac gcattcggga
     3241 attcctcgcg gtcgcaacct acgactcgct gcaaaaggcc gagaatcggt atttcttccg
     3301 aaagcgcctt cccgacgagg agcagccagg catttacatg cgcgaagggg ccaccggcga
     3361 ggaccagctt ctggtcgatc cggccgcgtg gggaaccggc aagtacaccg cggtgaagcc
     3421 tctgcgcgtc tcgggggacg gacggctact gctctacgag atcaaacaag ggggagaacg
     3481 caccggaaga ttcgcgatat tcgacgtcgt cgcgcgccag acgctccccg atgttcttcc
     3541 tcgtgggtac ttgcgcgggt tcgcatttgc gccagacggg aagagcttct actacgttca
     3601 cgaggcgctg gacgcggaac ggccctttta ccgggcggcc tatcagcaca cacccggcac
     3661 gccgttcagc gaggaccggg agattttctg cgcaggagag gacgccaacc tccggctgtg
     3721 tctcatcggc gatgaggagc gcctcggatt cctggtctac cggttcggtg aaaaaacacg
     3781 caccgatttc tatctgcgat cgtttgaggc gtgtggcgcg ccgcaagtca ttgttgccga
     3841 cgctcgatac agtcttgggc ctctgttgac ctccgggaaa atcttcgcca tcactgaccg
     3901 caacgcgccc aacctgcgca tcgtggagct tcggctccaa aagaacaaag agccgctgtg
     3961 ggtcgaaatc gttccggaaa gcgatgcgcg aatccatcaa tgggccgttg cacgcgaccg
     4021 catcttcgtc tcctacgtcc gaggcacagc cttcgacgtt tccatccttg atttcgcggg
     4081 agaacctgtg gggaagatgc cggttcagag cggcgagaca atccggctca tgggcggctc
     4141 ggccaagagc gacgagctgc tggtcgaagc cgaatcgttt accgagccga tcacgatctt
     4201 ccgcctgccg gtgaacggcg ggcaacgcgc gccgtgggca aaaagaagta ttcccttcga
     4261 tgcctcacgc tacgagcaca cacaagtctg gtatacagcg aaagacggga cgcgaattcc
     4321 gatgttcgtc ctgggccgca aggatgttct cgccgcagga agtcatccta cgatcctgac
     4381 gtcttacggc ggttacggag tctcgatgac gccgcaattc agcgtctttg tcgcgttcct
     4441 ggtcgagcgc ggatgcctgt ttgcgttgcc caacattcgc ggcggatcgg aattcggcgc
     4501 ggcctggcac attgcggcca aacgccacaa ccgccaaacc gcttacgacg attttctgtg
     4561 cgcagccgaa tggctgatcg cgtcagggcg aagcgctccc ggaaagctcg cgatcttcgg
     4621 agggtcgaat tccggcttgc tggtggccgc cgccatgacg cagaggcccg acctgtttcg
     4681 cgcggtcgtt tgcatggtgc ccatgctcga catgctgcgc taccacttgt tcgacaacgc
     4741 gcatgtctgg agggaagaat tcggcacggc ggaggacccg gacgatttcg ccgttctcgc
     4801 gagctactcg ccctaccatc gcgtccagga cggcacggcg tatcccgcga cgatgattgt
     4861 ttccggggac gccgacagaa attgcaaccc cctgcacgcc cggaaaatgg cggctcgcct
     4921 gcaggcggca aatgcgtccg ggcatcccgt gctgctcgac tacagtccgt tccgcgggca
     4981 ttccccggtg cttcccttga gcgagcggat cgaatcgctc accgaccgga tggcgtttct
     5041 ctgcgatcaa ttggggctgt ccgtttgaag gaggctggcg atgcggtttc tgagcctgaa
     5101 ggcgtacgga aaactgattc tcttcgatct ctatctggcg cgcgggaatt tcgccgcgct
     5161 gtatggcaag gtgcgcaact gccgggtccg cagccaggag ccaattcacg atgccgctgg
     5221 acgaatctgt gcggcgatgg acatggcgtg catttggtac tggaaagaag cgcggtgcct
     5281 gcaacggtca gcggcgacag catgcttgct caagcaatac ggagtgcgcg ctcagatggt
     5341 cattggcgcg cagcaactgc cgttcaaggc gcacgcctgg gtcgaagtcg atggccgggt
     5401 cgtgaatgac aaaccgtaca tgcgggaaat gtacgccgtg ctcgatcggt gctgacgccg
     5461 atcgaagggc ggagccaggg gaacagatca tgagcatgca gtttggaaga tggaatttcg
     5521 agggcatgcc g
//
LOCUS       DATNDT010000468         2920 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2591894, whole genome shotgun sequence.
ACCESSION   DATNDT010000468 DATNDT010000000
VERSION     DATNDT010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2920)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2920)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2920
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2591894"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..414)
                     /locus_tag="VN788_15470"
     CDS             complement(154..414)
                     /locus_tag="VN788_15470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21982.1"
                     /translation="MTSPLSGPGNLQLPDWQNDFETAARQAAQTPGRIQAMKAAVLTR
                     LRAKLENPPGPLERIALNDAILLLRAMRSGSPPNACLETDLE"
     gene            649..2061
                     /locus_tag="VN788_15475"
     CDS             649..2061
                     /locus_tag="VN788_15475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012405404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU21983.1"
                     /translation="MTPAEQTAIHDQTALISARLDRLPTTPYIWSLLLLISLGGCFEF
                     YDLFVTAYIGPGLVRSGLFSAASASFFALHGLASFVSATFAGLFVGTIAFGFAADRFG
                     RRAVFTYSLLWYTACSIVMAFQISTLGVLVWRFITGIGIGIELVTIDAYIAELMPKKI
                     RGRAFALNQMVQFTTIPLVALISWLLVPRSPFGIDGWRWVMLIGSAGAIFVWFIRRRI
                     PESPRWLIARERIAEAERAVAKMEKRVAVELGAELPPIIAEIPEEKSPDASVKFVQIF
                     RPPYLRRTLMLMVFHLAQTVGYYGFASWVPTLLIAAGIRTTASLQYSFIIAISAPLGP
                     LLVQGIADKLERKWQIVWSAICIGGFGLLFARQSEPGWLIVLGVALTCSNNWMSVAFH
                     AYQAELFPTRVRAQAVGFVYSWSRFSAIFTSLMIGFFLERFGVGGVFGFIAVSMFAIV
                     LAIGIFGPRTRGLALESISH"
     gene            complement(2119..>2920)
                     /locus_tag="VN788_15480"
     CDS             complement(2119..>2920)
                     /locus_tag="VN788_15480"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HXU21984.1"
                     /translation="YHDKVIVIDRRTLYVLSFNFTHLDIDHSRGFGIATHNSKWVAEA
                     VKLLEADSKRARYSCGLETFVVSPVNARKALGNFLKRAHKQLLIYDPKLSDKEMIRIL
                     EDRAKAGVEIRAIGQAKANLKVKKLSKLRLHTRTIIRDGRQAFIGSQSLRAAELDSRR
                     EVGLIIRDAKIVKKLVDIFESDWTIGGKKIPPSNADKPKVPEKDSDKAAKILIQELHP
                     IAATVRKAIKKAVAKAGDEVLEDGSARDTVKEVVKKAVKQAVKEAAKN"
BASE COUNT          531 a          807 c          796 g          786 t
ORIGIN      
        1 ccatcgatca gtctgatatt cgccgtttgc atgagtgagt tgacgccaac tatttcaaac
       61 acaccctcgt gctctgcgct acggacgcgt tcgccattct ttggagcaat cgctttcgtt
      121 gcagtggcca tcttcccctc cattgctttg cagctattcc agatctgttt ccaagcaggc
      181 gttaggcggg cttccgcttc gcatcgcccg caatagaaga atcgcgtcgt tcagtgcgat
      241 gcgctcgaga ggtcccggag gattttcaag ctttgctctc agtcttgtta agacggccgc
      301 tttcatcgcc tgaattcgac caggcgtttg cgctgcctgt cgcgccgccg tttcaaaatc
      361 gttttgccaa tcggggagtt gcagatttcc gggtcctgaa agaggcgagg tcattgacgt
      421 acctccgcgc ttactacttc gtgttgggag agagtgtgtt ccttattatc cgttcgtgtc
      481 aaccccttga ggctgcggcc acgccgcatc ggttaggaga tggtactccg tcgagcaaga
      541 gtagcctctg ccgccggtgg gccctgtgtt ccgcccaatc ccggcctcca gccgacccga
      601 atgcactatc cccagtgctc ggcgattgct agaatccgcg aaacctcgat gacgcctgcc
      661 gagcagacag cgattcacga ccaaaccgcc ctcattagcg cgcggctcga ccgactgccc
      721 actacgccct acatctggtc acttttacta ctgatttcgc tcggtggatg tttcgagttc
      781 tatgatcttt ttgttacagc ctatataggt cctgggttgg ttcgtagcgg actgttctcg
      841 gccgcttcgg cctcgttctt cgcgcttcat ggactggcca gctttgtatc tgctacattc
      901 gccgggcttt tcgtcggaac aatcgcattc ggatttgccg ccgatagatt cggccgccgg
      961 gccgttttta cctattcttt gctgtggtat acggcgtgtt cgatcgttat ggccttccag
     1021 atcagcacgt taggagtctt agtctggcga tttatcaccg gcatcggcat tggcatcgag
     1081 ttggtaacaa tcgatgcata catcgctgaa ctgatgccga agaaaatccg cggccgcgcc
     1141 ttcgccctca atcagatggt gcaatttacg acgataccgt tggttgcgct gatttcctgg
     1201 ctactggtgc cgcgcagccc tttcggcatc gacggatggc gctgggtgat gctgatcggt
     1261 tcggcaggcg cgatatttgt ttggtttatc cggcgaagaa ttccggagag tcctcgctgg
     1321 ctgatcgctc gcgagcgcat cgccgaggcg gaacgcgccg tcgcgaaaat ggaaaagcga
     1381 gtggcagtgg agttgggcgc tgagcttcct cccatcatcg cagaaattcc cgaggagaaa
     1441 tcaccagacg cgagcgtaaa attcgttcaa atttttcgcc cgccgtatct gcgacgaacg
     1501 ctcatgctca tggtttttca tttggcgcaa actgtcggtt actacggttt tgcttcgtgg
     1561 gtgcctacgc tgctgattgc ggcgggcatc cggacaactg ccagcctaca atactcgttc
     1621 atcattgcca tctccgctcc tctggggcca ctgctcgtcc aagggattgc cgacaagctc
     1681 gaacgcaagt ggcaaattgt gtggagcgcg atttgcatcg gagggtttgg cttgttattc
     1741 gcgcgccaga gcgagccagg ctggcttatc gtgcttggcg tagcgttaac ttgttcgaat
     1801 aactggatgt ccgtggcatt tcacgcgtac caagctgagt tgtttcccac gcgagtgcgg
     1861 gcacaggctg tgggcttcgt ctattcctgg agccgtttca gcgcgatctt cacgagcctt
     1921 atgattggct tttttcttga gcggttcggc gtaggtggcg ttttcgggtt catcgcggtg
     1981 tcgatgttcg caatcgttct ggcaatcgga attttcgggc cacggacgcg cggtctggca
     2041 cttgagtcaa tttcccattg atatgcggtc agtcaaactc tcgagtggac ccctcgtttt
     2101 cagccagcga gaatgtcact agttcttggc ggcctccttg accgcctgct tcacagcctt
     2161 cttgacgact tccttcacgg tgtctcgagc acttccgtct tcgagcacct catcgcccgc
     2221 tttggctact gctttcttga tggccttcct aacggtggct gcgatgggat gcagttcctg
     2281 tattagaatc ttggcggctt tgtcggagtc tttttccggc accttcggtt tgtcggcatt
     2341 ggacggcggg attttctttc cgccaatggt ccagtcagat tcaaagatgt ccacaagctt
     2401 cttgacgatt ttcgcgtctc gtatgatgag accaacctcg cggcgcgaat ccagctcggc
     2461 ggcgcgcaga ctctggctgc cgatgaaggc ctgacgtcca tcccgaatga tggttcgcgt
     2521 gtgcaaacgt aatttggaaa gttttttgac ctttagatta gccttggcct gcccaatggc
     2581 tcgaatttca acgccggcct tagcgcggtc ttcgagaata cgaatcatct ccttatcgga
     2641 caacttcgga tcgtaaatca gaagttgttt gtgcgcccgc ttgagaaaat ttccgagcgc
     2701 ctttcgtgca ttgacagggc ttacgacaaa agtttccagt ccacaggaat accgggcgcg
     2761 tttcgaatcc gcctctaaaa gcttgaccgc ttcggccacc cacttggaat tgtgggtggc
     2821 gatcccgaat cctcggctgt gatcgatatc caggtgcgtg aaattgaagg aaaggacgta
     2881 caaagtgcgc cggtcaatga cgatgacctt gtcgtggtag
//
LOCUS       DATNDT010000469        13633 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3478037, whole genome shotgun sequence.
ACCESSION   DATNDT010000469 DATNDT010000000
VERSION     DATNDT010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13633)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13633)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13633
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3478037"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..2012
                     /locus_tag="VN788_15485"
     CDS             291..2012
                     /locus_tag="VN788_15485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21985.1"
                     /translation="MRRLIHRWLSRAIVFVGFLASGSLLQAQTPLVERLSPDTVFCVA
                     WRGTASFSGAEQKNHVLQLLQDPAFAPAWLSLAAAFQQRNQKAEGPAGALGFADITSL
                     LENPLVLGVSAVPNGAGSPGPNGQPSRFGFFIVYDATGKAGLIDKWKLSAQSGNKAHS
                     QATTYDFGGTSVEIRADGARATYSAQTAHYFVASDQKQIVEGLITRFRSADVNPGSLG
                     HLAPFRDLQKFVGSDVALEWYARIPDPGQWSIPPSGGKSAVQFAKTIHLEKIHAVGGG
                     VSFAAEAMHIRGAVLGDTSPGGVFDLAGPSTATFQTTAVVNGSSTFSMSHFNLAALYT
                     LAHGAIVGTLSSQQMANVSAVEAVGQSYLGMPIGDALGLMSGEIASFSTYSDDGSAQR
                     VFAATIRQPEAVLRILRAVAGSMIVAEDSSGTATFLDIAYPYKDPKTGTQRRKFYYLA
                     VTPQMLVGAPRKAMLRETIQRLGTHADMSPPAGLLASPDFSRMRSLLPEKLSGLSGSD
                     LTQIPWDKLVSQLEDQIAQGTKQKNGQQLPDLSWLKPDTISRHLHIALSGWWKDSGGV
                     YFDSYLQ"
     gene            2050..3057
                     /locus_tag="VN788_15490"
     CDS             2050..3057
                     /locus_tag="VN788_15490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU21986.1"
                     /translation="MAAVILSLFPACLGAAQKSASPQTPPLRSATELVKLDTSVLDQR
                     GKFVAGLTARDFRVLDDGIEQPIAFFAPVQTPAQILVVLETSPAVYLIEKEHIAAAYA
                     LLDGLAPNDRVALATYDAIPHGVLGFTEDKSALLAALSQVQYTIGMAELNLYDSISTV
                     LDWLGPSAGKKALVLLSTGLDSSPPRWDALVAKLRREDVVIFPVALGGSLRGEPGRNS
                     KPRRKSSKRDGKQNLGSPPAADSEIFAKGDAALLSLASMTGGRAYFPKSANDFVPIYR
                     QIAYELRNEYVLGIQPAQDGEFHSLKVEVIANDGKSSVSHFNAPAYQIFVRAGYLAPK
                     P"
     gene            3105..4310
                     /locus_tag="VN788_15495"
     CDS             3105..4310
                     /locus_tag="VN788_15495"
                     /inference="COORDINATES: protein motif:HMM:NF012803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HXU21987.1"
                     /translation="MDRKLRVLVLPLIILTVGLAIRPAPQPTNHSTFPQQPPAPLRFT
                     FGGNATEIPATFIDSLVFLQVHVNQSEPSLFELDSTAAVSSIDPERAIEIGIGRTPVP
                     LLNLSGVDISLGSLAERAKKDFAAYVGRPYEGTIGNDVLADAVVEIDYARQTVRLYDP
                     ATYQYSGRGKTFHLSLASGEPVVQAKFTETNGKTFEGNFGVDTALDASIVISGKFGQM
                     HHLHFARTKTIPATDIELGGVQQPIFARIMQLSVGPYDIRDPIAVFSRGDSPAGDNPR
                     LAGQLGGNLLRRFTVTFDYPHQRMILDPNSDFRTDDLEDMSGLTIVATGSNLKTFEVN
                     EVRSGTPGADAKIQKGDVIAGVDDEAAADLSLAAIRKLFRQRGAKYKLLIQRNGQTLA
                     VTLQTRRLL"
     gene            complement(4358..6829)
                     /locus_tag="VN788_15500"
     CDS             complement(4358..6829)
                     /locus_tag="VN788_15500"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU21988.1"
                     /translation="MASEKVEEMARNHQPQHESLPPEKLRWRCDPATVPFETTAEIAP
                     ISGDMGQERALRALRMGVELTASGYNLFVCGLSGTSRGGMIVRMIEDLRPQTEPAPDR
                     CYVNNFKNADRPRLLTLPRGQANAFKKEVESGIDFLRRRIPQVFEGEPFQRQKTRIVE
                     RFTVREKELMDDFTRRIAREQFALGRMQVGAVALPEIFPVLEGQMVPIEEIPKMVQDG
                     KLENNAAEEIERKYDQFRQEFTVVYRRTLSLSRELASEMSYLEQEAASVLVDGVIEEL
                     KEKYPSSSISEYLEEVRHHILDNLEPFKEREGEDEQQPANDGGAPKPERTERDPFRVY
                     GVNVILAHVESEKCPVVFETIPTYANLFGTIHRSYDTRGGWNSDFMDLRGGSLLRADG
                     GFLVMYALDTLTEAGVWRTLKRTLNHGKLEIQPVDVFFPFSTAALKPEPIDVHAKIIL
                     IGDRDMYEMLYDYEDDFKKIFKVRVEFDEEMKWSEEVACQYGGRLRKLVDDEKLLHFD
                     RSAVAAILEHGVRLAGRRGKITTRFFDLADLARESSYAAKLDASQTISAEHVRRALDA
                     KVDRHNLLETKIREMIEQNLVFIDTTGGRAGQVNGLSVMEIGGYAFGKPVRITASVAL
                     GKTGIINIERESNLSGRFHDKGVQIISGYIRRVFSQDKPLSLAASLCFEQSYSGIDGD
                     SASSTEIYALLSALADLPVRQDLAVTGSVNQQGDIQPIGGVNQKIEGFYDVCRLKGFT
                     GSQGVLIPAENIDDLMLRDEVIAAVAKGIFHIYPVATIEQGIEILTGVRAGKRSPSGK
                     FEPGSVFAKADARLSNMATTLRRFE"
     gene            complement(6962..8023)
                     /locus_tag="VN788_15505"
     CDS             complement(6962..8023)
                     /locus_tag="VN788_15505"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HXU21989.1"
                     /translation="MTTETTAHKRLGSILFFGIVILLVYLVYLVVSPFVVPLAWAAVL
                     VVVSYPAYERVAARLGRTTGAAVTTTAVTLILIVPSLFVMIAFVRQGVNAAQSIQFQV
                     AIGHFAWLNELWLKIQQRFPAANGRDLSESLRHYGELAATFVAARIGTILKHSAIFVF
                     DLGVTILAMFYFYRDGAAMIARLRKVLPFRSAYRDRMLGETHELIFASVTSSLVAAAV
                     HGALGGLAFAVTGVKAPIFWGVMMGFLSFVPLVGSALVWLPISISLMISGHLIRGLIL
                     VAFCSVIVGLVDNLIRPWLISGRAEMSGLVVFISVLGGISAFGLLGVVLGPIVVAAAT
                     SLLDLYASEPISTNKQLET"
     gene            complement(8187..8627)
                     /locus_tag="VN788_15510"
     CDS             complement(8187..8627)
                     /locus_tag="VN788_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21990.1"
                     /translation="MWYVNKLFNQPQVESLSAKGFLVRSSGGVVRIEKYGCGAELRKE
                     PQGNYRMTIIPSVMLQGQFTHLWDAGYQKFLLTHEGRKYPIRVGQLADLRRFNEELRT
                     ALGVPTYYNEAIGSTCHYSVYDRVKGRTGDVPDETVGAHEEADH"
     gene            8746..10614
                     /locus_tag="VN788_15515"
     CDS             8746..10614
                     /locus_tag="VN788_15515"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU21991.1"
                     /translation="MTAAQALRVFLFLFAAGFVAAPAHAQTAPNGSALFQTHCATCHE
                     GAAAASNRAPTRATLAQKSQEEILRALESGPMVIYGNRMSEVERHAIAAFLSSNTAST
                     AALSRTNLCATKEPITSAGAMSPGNWNGWGLDIVNSRYQTHTTISPGNVSGLKVKWAF
                     GIPDASTAYGQPTVFAGRVFFGSGNGTVYALNADSGCTLWTYKAGTTVRTAITVSAAG
                     KDRYLAYFGDGEANLYAVDAETGKLAWKVKVGQHRSARITGAPQFYGNRLYVPIASNE
                     ELNGGDPNYPCCTFRGNVVAVDALTGKQIWRAYAIAEKPGPSKPGTSHAKFSPAGAAI
                     WDTPTIDAKRGVLYVGTGDAYVDPAAEGTDAIVAFDLATGKRLWLQQKTPNDVFNFGC
                     IDPHHFNCPARPGPDVDFGSSPILQDLGNGHRVLLAGQKTGVMYGLDPDDAGKELWQA
                     RVGQGSGLGGIEWGSATDGTNVYAANSDISTLTSSGANPPPPGGLAAIDIRTGKLVWK
                     TMPPKPACLGQSGCSAGQLAAVTAIPGVVFSGSMDGHLRAYSAKDGRIIWDFDTLPDF
                     PTVNGVKAHGGSMSGNGPVVSGNMIYVASGFDVTAGMPGNVVLAFEDARAVSAASR"
     gene            10734..11600
                     /locus_tag="VN788_15520"
     CDS             10734..11600
                     /locus_tag="VN788_15520"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HXU21992.1"
                     /translation="MQPSRWYGVLLLAILAVAAVASTVAQQHHRARAPKTLRLYVFDC
                     GVIKGLNPALFNFKKDELAETELAVPCYLIADPKGTLMWDVGVIPDSAFKNDGKPVTQ
                     GPSTVTRTLKSQLAEIGYSPSDITYLAHSHYHGDHVANSNEFADSTWLVRKVERDAMF
                     AAKPPSLVNAANYSDLKNSKTIILAKDEYDVFGDGKVIIKSAPGHTPGHQVLILKLAK
                     TGPVMLAGDLYHYPEERKLNRVPTFEYNKEQSLASRAMIEDYCKKTGTQLWIEHDYVH
                     NSKLKKSPQYYD"
     gene            complement(11695..12963)
                     /locus_tag="VN788_15525"
     CDS             complement(11695..12963)
                     /locus_tag="VN788_15525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21993.1"
                     /translation="MGKLSMSLIFGFVLVLTVRPSVQAQTTPYAPADASTANPAPSGQ
                     APAEVLKKLSDLIHAGKYVDAQQLAAGLLLAYPADQRLITAKGLLDKAVSSSQSADTG
                     ASADQPTGSTASLPPGTNATALTGMDKVEYNSLIELARQAQQTTDLSQQKTLLQKFMS
                     ESRPFLEQHPDEMLVWQLRAASALSLNDPIEGYEAGQKLLSAGAANSNDPNLQHLMAQ
                     LNLQGWLDHKKAEIKEQEKGFAWLVGTWTSTWDWWWGEVHGRDQEQFVVTDSGIDGYL
                     IGGDGVRNPEPDFRGKMLDSGEIQWECYMPPADTGGMFVFRRVGGSVFTKVTIGRRSD
                     PQGYYAGNVHHFGYDPHSTDTGDKVFYPSGWQPVASYKLSADKRTMTIVILPQDVEPN
                     SKFARKFPVTLVLKKSGGQDEQLQSQQLPQ"
     gene            complement(13025..>13633)
                     /locus_tag="VN788_15530"
     CDS             complement(13025..>13633)
                     /locus_tag="VN788_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="papain-like cysteine protease family protein"
                     /protein_id="HXU21994.1"
                     /translation="SLRPAGADSAELPPYYGQLVKDIGKAESVTANASYMGAGRQTQD
                     DCVLYAIANGAHVPEKQVNAEFRKIVRDLGMDPIEERANPEIVVTEPQNGGRGGLNAA
                     EELLVADKFGKVVPVPADSYARAIASTKQPVITTIQLDKTGVQHKVVVTGVYRTADGK
                     IYYSVMDSNLGPDYHNSTGYVEKSNFEEHLASGGFVVLPAAN"
BASE COUNT         2983 a         4026 c         3623 g         3001 t
ORIGIN      
        1 gtcggcgagc cggtccaatt ctacgttcgc ggtgcgcgcg cgccctatga gattgttgtg
       61 ttcgatctta cgaaaaacga ggcgaagggt tatctaagca cgcccaagtc ggcctcggct
      121 gcccctcctg cggccaaccc tcccggtcac tagttgtacg ttggcgaata acgaacgcgg
      181 ccttcagatt cgcgcagcgc cattttcctg cccatgtcat gctactgaaa aatccgtaac
      241 ggatcgtttc ggtagctctg gttttgggta aggaggcgtg catgcatccc atgcgacgtt
      301 tgattcaccg gtggctgagt cgggcaattg tattcgtggg tttccttgca tcgggctctt
      361 tgttgcaggc acaaacgccg cttgtagagc gcctgtcgcc agataccgtc ttttgcgtag
      421 cctggcgcgg cacagcctct ttttctggcg ccgagcaaaa gaatcatgtt ctgcaacttc
      481 tgcaagatcc ggcctttgcc cctgcttggt taagccttgc cgcagctttt cagcaacgga
      541 atcaaaaggc ggagggtccc gctggggcgc tgggattcgc cgacatcacg tcattgctgg
      601 aaaatccact ggtgcttggg gtgagcgccg ttccgaatgg cgcaggatcc cccgggccaa
      661 atggccagcc atcgcgcttt ggattcttta tcgtttacga tgcaaccgga aaagccggcc
      721 tcatcgacaa atggaagctc tctgcccaga gcggcaataa ggctcattct caagcaacga
      781 cctatgattt tggtggaacg tcggtggaga ttcgcgccga cggcgcccgc gctacctata
      841 gcgctcagac cgcccactat tttgttgcgt ccgaccaaaa acagattgtg gagggtttga
      901 tcacccgctt ccgcagcgcc gatgttaatc ccgggtccct gggacacctt gcaccgtttc
      961 gtgatctaca aaagttcgtc gggtccgatg tggcccttga atggtatgcg cgcattccgg
     1021 acccgggcca gtggtctatc ccgccctcgg gcgggaaatc cgcggtacag ttcgccaaaa
     1081 ctattcacct cgaaaagatt catgccgtcg gaggcggtgt gagcttcgcc gcagaagcga
     1141 tgcacatcag gggcgctgtc ttgggcgaca cgtcacctgg aggcgtcttt gatctcgccg
     1201 ggcccagcac cgccaccttt caaacaacgg cagttgtgaa cgggagctcc acattcagca
     1261 tgtcacactt caatctcgca gcgctataca ccctcgccca tggcgccatt gtcggcaccc
     1321 tgagttcgca acaaatggct aatgtcagcg cagtcgaagc tgttggccaa agctatctgg
     1381 gaatgcctat cggcgacgcc ctcggcctaa tgagcggcga aattgcttct ttctccactt
     1441 attccgacga tggcagcgcg caacgggttt tcgccgcgac catacgacaa ccggaggccg
     1501 ttctgcgaat cctgcgagcc gtcgccggct caatgatcgt ggcggaagac tcttccggga
     1561 ccgctacctt cctggatatc gcatatccct acaaggaccc aaaaaccggg acacagcgga
     1621 gaaagttcta ttacctcgcc gtcacccctc agatgttagt cggtgcgccg cgaaaagcca
     1681 tgttgcgtga gactattcaa cggctcggta cccatgcgga tatgtcgccg ccagccgggc
     1741 ttttggccag cccagacttt tctcgaatga ggtcgctcct ccccgaaaaa ctttccggat
     1801 tgagcggttc ggaccttact caaattccgt gggataaatt agtctcacag cttgaggacc
     1861 agattgcgca aggaaccaag cagaaaaacg ggcagcaact gccggatttg agctggctca
     1921 agccggatac aatctcgcgg caccttcaca ttgccctgag cggatggtgg aaagattccg
     1981 gcggcgtgta cttcgattca tatcttcagt aaggtgaaaa attttctcgg gaccggtcgt
     2041 ttctcgctaa tggcggccgt cattctgtct ctctttcccg catgcttggg ggctgcacag
     2101 aaatcggctt caccgcaaac gcccccgctg cgatccgcga ccgaactcgt aaaattggac
     2161 accagcgtcc tcgatcagag ggggaagttt gtcgccgggc tcacggcgcg cgattttcgc
     2221 gtcttggatg acggcattga gcagccgatc gcctttttcg ccccggtcca aaccccagca
     2281 cagatcctcg tggtgcttga gacgagtccc gccgtgtacc tgatcgaaaa ggaacacatc
     2341 gcggctgcct atgcactttt ggatggcctg gcgcctaacg accgggtcgc gctggcaacc
     2401 tacgatgcaa taccgcacgg agtgttgggt tttacggaag acaaatcggc gctcctggca
     2461 gctttatccc aagttcagta caccatagga atggcggaat tgaacttata cgattcaatc
     2521 tcgaccgttc tcgactggct tggaccatcg gccgggaaaa aggcgctggt actcctttcc
     2581 acgggactcg attcttcacc tccgcgatgg gacgccttag ttgcgaagtt gcgccgagaa
     2641 gacgtcgtta ttttcccagt tgctttggga ggctcgttgc gaggagagcc tggccgcaac
     2701 tcaaagccgc gaaggaaatc atcgaaaaga gatggaaaac aaaacctcgg atctccacca
     2761 gctgccgatt ccgagatttt cgccaagggt gatgcagcgc tgctctcatt ggcatccatg
     2821 actggcggtc gagcatactt tcccaaatcc gcgaacgatt tcgttccgat atatcgccag
     2881 atcgcttacg aactgagaaa cgaatatgtc ctaggaattc agccggccca ggacggagaa
     2941 ttccactcgt tgaaagtcga agtgatcgcc aacgatggta aatcgtcggt ctcacacttc
     3001 aacgcgccgg catatcagat ttttgtgcgc gccgggtatt tggctcctaa gccttagttt
     3061 cgtatactct caatcccaac gtgtaccggt tcaccagggg taagatggat cgaaagcttc
     3121 gggtattggt gctgccctta atcattctca ccgtcgggtt ggccattcgg cccgcgccac
     3181 agccaacgaa tcactcgacg tttccgcagc agcctccggc cccgctccga tttaccttcg
     3241 gaggaaatgc aaccgaaatt cctgcgacct tcatcgacag tctcgttttc ctccaagtgc
     3301 acgtgaacca gagcgagccg agtctcttcg agctggattc gactgccgct gtatcgtcga
     3361 ttgatcccga acgcgcaatt gaaattggaa ttggacggac gccggtgcct ttgctcaacc
     3421 tgagcggagt cgatatttct ttgggttcct tggcggagag agccaaaaaa gatttcgctg
     3481 catacgtggg ccggccgtat gaaggcacga ttggaaatga cgttctcgcg gatgctgtcg
     3541 tcgaaataga ctacgcaagg caaaccgtcc gcttatacga tcccgcgacc taccaatact
     3601 ccggcagagg aaagaccttt catttgagcc tggcctccgg cgaacccgtg gttcaggcaa
     3661 aattcaccga gacgaatggc aaaacatttg agggcaattt cggagtagac accgcccttg
     3721 atgcctcgat cgtaatttca ggcaagttcg ggcaaatgca tcatctgcac ttcgcacgta
     3781 caaagacaat cccggccacc gatatcgaat tgggcggagt gcaacagccg atattcgcaa
     3841 ggatcatgca attgagcgtt ggcccatacg atattcgaga tcctatcgct gttttttcgc
     3901 ggggagattc gccggccggc gacaatcccc gcctcgccgg acagctggga ggaaatctgc
     3961 tgcgcaggtt caccgttacg ttcgattacc ctcaccagcg catgattctg gatcccaata
     4021 gcgattttcg caccgacgat ttggaggaca tgagcggtct cacgatcgtt gcaacaggtt
     4081 cgaaccttaa gacctttgag gttaacgagg ttcgctccgg gacccccgga gctgatgcca
     4141 agattcagaa aggggatgtg atcgctggtg tggacgacga ggccgcggcg gatcttagcc
     4201 tggcggcgat tcgcaaatta ttccgccagc ggggcgcgaa gtacaaactc ctcattcagc
     4261 gaaacggcca aacgctcgcg gtgacgcttc agactcgccg ccttctctga ttggcgcgcg
     4321 cattcggcgg tcgaccaatc tctcggaccg gacgctctta ttcgaatcgc cgcaaagtcg
     4381 tagccatatt gctcaatcga gcgtccgctt ttgcgaaaac gctaccgggc tcgaatttcc
     4441 cggaaggact tcgcttgccg gcgcggaccc cggtaaggat ctctattcct tgctcgattg
     4501 tcgcaaccgg atagatatga aatatgccct tcgccacagc cgcgatcact tcatcccgca
     4561 gcattaaatc atcgatgttt tccgccggga ttaaaactcc ctggctccca gtgaaacctt
     4621 tgaggcggca tacgtcgtaa aagccctcga tcttctgatt cacgccgcca atcggctgaa
     4681 tatcgccctg ctggttcacg gagcccgtca ccgccaagtc ctgccgcacc ggcagatcgg
     4741 ccagcgcaga aagcaatgca taaatttccg tggagctggc gctatcgccg tcgatgccgg
     4801 aataggattg ttcgaagcac aagctcgccg ctaacgataa cggcttgtct tgagaaaata
     4861 ctcgccggat gtaaccagaa ataatttgga cgcccttgtc gtgaaagcgg ccgctcagat
     4921 tggattcgcg ctcgatattg ataatgccgg ttttcccgag agccactgac gccgtaattc
     4981 ggacaggttt gccgaacgcg tagccgccga tctccatcac actcaacccg ttcacttggc
     5041 cggcgcgtcc ccctgtggtg tcaatgaata cgaggttttg ttcgatcatt tcgcggattt
     5101 tagtttcgag cagattgtgc ctgtccacct ttgcatccag agcgcggcgg acgtgctcgg
     5161 cgctgatggt ctggcttgca tcgagcttcg ccgcatagga cgattctcgc gcgagatcgg
     5221 cgagatcaaa aaaacgcgtg gtgatcttcc cccgccttcc agcaaggcga actccgtgct
     5281 cgagaatcgc tgccaccgcc gaacggtcga agtgcagaag tttttcgtca tctacaagtt
     5341 tccgcagacg tccaccgtac tggcaagcga cctcttcact ccatttcatc tcttcgtcga
     5401 attccacacg gaccttgaaa atctttttga aatcgtcttc gtagtcgtac aacatttcgt
     5461 acatatcgcg atcgccgatc aggatgattt tcgcgtggac gtcgatcggc tccggtttca
     5521 gcgccgcggt gctgaacggg aagaaaacgt ccaccggttg aatctcgagc ttcccgtgat
     5581 tcaaagttcg tttcagcgtg cgccacacgc ccgcttccgt cagcgtatcc agcgcgtaca
     5641 tcaccaggaa gcctccatcc gcccgcagaa gagagcctcc cctcaggtcc atgaagtccg
     5701 agttccatcc gcctcgcgtg tcgtagctgc gatgaatggt gccgaagaga ttcgcgtagg
     5761 tcggaatcgt ctcaaatacc accggacatt tttcgctctc cacgtgcgcg aggatcacat
     5821 tcacgccgta aacgcgaaat gggtcgcgct cggtacgctc cggcttcggc gcgccgccgt
     5881 cgtttgccgg ttgctgctcg tcttcgccct cgcgctcttt gaaaggctcc aggttgtcga
     5941 gaatgtggtg gcgaacctcc tccaggtatt ccgaaattga ggaactcgga tatttttcct
     6001 tcagctcctc gataacacca tcgaccagaa cggacgcggc ctcttgttcg aggtaactca
     6061 tttcgctggc cagctcgcgc gaaagagaaa gggtccgccg gtacaccacg gtgaactcct
     6121 ggcgaaactg gtcgtatttc ctctcgatct cttcagcggc gttgttttcc agcttcccgt
     6181 cttgcaccat cttgggaatt tcctcaatgg ggaccatctg cccttcaagc accggaaaaa
     6241 tttccggcag cgcgacagct cccacttgca ttctcccgag cgcaaactgc tcccgcgcga
     6301 ttctgcgcgt gaagtcgtcc atcaactcct tttctcttac tgtgaatcgc tccacgatcc
     6361 gagtcttctg ccgttgaaac ggttcgcctt cgaacacctg cggaatgcga cgccgcagaa
     6421 aatcaattcc cgattcgact tcctttttga aagcgttggc ctgtcctcgc ggcagtgtca
     6481 gtaggcgtgg ccggtcggca ttcttgaaat tatttacgta acaccggtcc ggtgccggct
     6541 cggtttgtgg acgcaaatcc tcaatcattc gaacgatcat tccgccgcgg ctggtaccgg
     6601 aaagcccgca cacgaataag ttgtatccgg aggcggtcag ctccactccc atgcgcagag
     6661 cgcgaagggc tcgctcttgg cccatgtcgc cggaaatcgg agcgatttcg gcggttgttt
     6721 cgaaagggac cgtggcagga tcgcaccgcc agcggagctt ctcaggaggc aagctttcgt
     6781 gttgaggctg gtggtttctc gccatctctt cgactttctc ggaagccatc ctaagctcag
     6841 ggacgaggtt cgtcctcgcg cggcccgctt tcttcgagat tctgaattcc ccgaactggt
     6901 tgtttgttca atagctcttc aagggccgct actctagcac acgcggccag agtggttcct
     6961 actaggtttc caattgttta ttagtggata tcggctcgga agcatacagg tcgagaaggc
     7021 ttgtagcggc ggccaccaca atcggcccta aaaccactcc gagtagaccg aacgcactga
     7081 taccgccaag cacgctgatg aatacaacta gcccgctcat ctccgctcga ccactgatca
     7141 gccacggccg gatcagatta tcgacgagac ccacgatcac tgagcagaag gccaccagaa
     7201 tcagtccacg gatcaaatgt cccgagatca ttaggctgat cgaaatcggg agccacacga
     7261 gggcagatcc tacgagaggg acaaacgaca aaaagcccat catcacgccc caaaaaatag
     7321 gcgccttaac gccggtaacc gcaaatgcca gtcctcccaa ggccccatgc actgccgcgg
     7381 ccaccaagct ggacgtgacg ctcgcaaaga tcagttcgtg cgtttcgcca agcattcgat
     7441 cccggtaggc gcttcgaaaa ggcagcacct ttctcagtcg cgcaatcatg gccgcaccat
     7501 ctctatagaa ataaaacatc gctaggatcg ttactcctaa atcgaataca aaaattgcgc
     7561 tgtgcttcaa tatggttccg atcctcgcgg caacaaacgt cgcggctaat tcgccatagt
     7621 ggcgaagcga ttccgaaaga tcacggccgt tggcggccgg aaatcgctgc tgaattttta
     7681 gccacaattc attcagccac gcgaaatgac cgatcgctac ctgaaattgt atcgactgcg
     7741 ctgcgttcac gccttggcgg acaaatgcaa tcatcacgaa aagtgagggg acaatcaaaa
     7801 tgagcgtcac agcggtcgtg gtaacggccg cccctgtggt ccggccgaga cgcgccgcca
     7861 cgcgctcgta cgcgggataa gagaccacca ccagaactgc ggcccaggcg agcggaacca
     7921 cgaacggcga aaccacgaga tagactagat aaacaagcag aatgacgatg ccgaagaata
     7981 ggatactgcc taaccgcttg tgagccgtcg tttcggttgt caaatgcccc tcgcgtgggc
     8041 taccaggtag tcagggtgat agttgcattc accgcgtttg tcaactccgg gaagacttgc
     8101 gggaatcgcc tggctcgatg aggctcaggg agcgatcttc gcagcatttg gaaagagaac
     8161 tgcgcaacta ctattctaga gcgagcttag tggtccgctt cctcatgcgc gccgacggtt
     8221 tcatcgggca cgtcaccggt gcggcctttc acgcgatcat acacgctgta gtggcaggtt
     8281 gaaccgatgg cctcgttgta ataggtcggc acgcccagag cggtgcgtag ctcttcatta
     8341 aagcggcgca aatcggcgag ttggcccacg cggatgggat atttgcgacc ttcgtgcgtc
     8401 agcaggaatt tttggtaacc cgcatcccag agatgcgtga actggccctg caacatcacc
     8461 gacggaatga tcgtcatccg gtaattgcct tggggctcct tgcgaagctc cgcgccgcag
     8521 ccatatttct cgatgcggac tacgccgccc gagctgcgca caagaaatcc cttcgccgaa
     8581 agcgactcca cctgaggctg attgaacagc ttgttcacat accacattgg tccatcagaa
     8641 tatggattcc gctgccatcc tgtaaagccc aaaatattgc gacgcgggcg caaactctat
     8701 ataacctctc cccagcgaaa caggcggagc gctttaggag gttgaatgac ggcggcacag
     8761 gccctgcgag tattcttatt tcttttcgca gcggggttcg tagctgcgcc cgcccacgcc
     8821 cagactgccc cgaatggatc ggcgcttttc cagactcact gcgccacgtg ccatgaaggc
     8881 gcggcagccg caagcaatcg cgcacccacc cgagccacgc tcgcccaaaa atcgcaggaa
     8941 gaaattctgc gcgcgctcga atccggcccg atggtcattt acggcaacag aatgtccgag
     9001 gtggagcgcc acgccatcgc agcgtttctc tcgtcgaata cggcaagcac agccgcttta
     9061 tcaaggacaa atctttgcgc caccaaagag ccgatcacgt ccgctggcgc aatgagcccc
     9121 gggaactgga acggctgggg cctcgacatt gtgaattcgc gctatcaaac acacaccacg
     9181 attagcccgg gcaacgtatc gggattgaaa gtgaaatggg cgttcggaat tccagacgcc
     9241 agcaccgcct acggacaacc tacggtattt gcgggccgtg tatttttcgg ttcaggcaat
     9301 ggcacagttt acgccctgaa cgccgatagc ggatgcacgc tatggacata caaggccgga
     9361 acaaccgtgc gaacggcaat cactgtgtcc gcggccggga aagaccgcta cctcgcttat
     9421 tttggggatg gcgaagccaa tctctacgcg gtagacgcgg aaaccggaaa gcttgcctgg
     9481 aaggtcaaag ttggccagca tcgctcggcg aggattacgg gggcccctca gttctatggc
     9541 aaccgccttt acgttcccat cgcgtcaaat gaggaactga acggcggcga tcccaactat
     9601 ccctgctgca catttcgcgg aaatgttgtc gcggtcgacg cactgaccgg caaacaaatc
     9661 tggagggcct acgcgatagc ggaaaagccc gggccgtcca agcccggcac cagccacgcg
     9721 aaattcagtc ccgccggcgc agctatctgg gacacaccca ccattgacgc aaagcgcggc
     9781 gttctctatg tcggaacggg tgacgcatac gtggatcccg cggccgaggg cacggacgca
     9841 attgtcgctt tcgacttggc caccgggaaa cgcctctggc tgcagcagaa aactcccaac
     9901 gatgttttca actttggatg catcgaccct caccatttca attgcccagc gcggccgggc
     9961 cccgacgttg attttggctc gtcgccaatc cttcaggatt tgggtaacgg tcatcgcgtt
    10021 ttgctggcag gccaaaaaac cggcgtgatg tacggactcg atccggacga cgccggaaag
    10081 gaactctggc aagctcgtgt gggccagggc agcggcctcg gaggaatcga atggggctcg
    10141 gccacggacg gaacgaacgt ctacgccgcg aattccgata tttcgacgct tacctcttcg
    10201 ggggcaaatc ccccgccgcc aggaggcttg gcggcaattg acatccgcac cggaaagctc
    10261 gtctggaaaa cgatgcctcc gaaaccggcc tgtctcggcc aatcaggatg cagcgcggga
    10321 cagctcgccg ccgttaccgc aattcccggc gtggtatttt ccggctcgat ggacggccac
    10381 ctccgcgcct attcggcgaa agacggcagg attatttggg atttcgacac gctgcccgat
    10441 ttcccaacgg tgaacggcgt aaaggcccac gggggctcca tgagcggtaa tggccctgtc
    10501 gtatcgggaa atatgatata cgtggcctcc ggtttcgacg taaccgccgg gatgccggga
    10561 aacgtggtcc tggcattcga ggatgcacgg gcagtttcgg ccgcctcccg ctgacgtctg
    10621 cgggtcgaca gctcttaaaa cctaactgca aagtttccag ctattgattt cggccagggc
    10681 cctagcaggt caactatcct tcgacctatc gaggtttgac catggagact caagtgcaac
    10741 cttccagatg gtacggcgtt cttcttttgg caatattagc cgtcgccgcc gtggcttcaa
    10801 cggtcgccca gcagcatcac cgcgcgcgcg ccccgaagac gctccgcctg tacgtcttcg
    10861 attgcggcgt aatcaagggc ctcaatccgg cgttgttcaa tttcaaaaag gacgagcttg
    10921 ccgaaaccga actggccgtt ccttgctacc tcatcgccga tccgaagggc acgctgatgt
    10981 gggacgtggg tgtaatcccg gattcagctt tcaagaatga cgggaagcca gtgacgcagg
    11041 gaccgtccac tgtcacgcgc acgctgaaat cgcagctcgc cgaaatcggc tactcaccat
    11101 cggacatcac ctatctggcc cactcgcact atcacggcga tcacgtcgcc aactcgaatg
    11161 agtttgctga ctctacgtgg ctggtgcgga aagtggagcg cgacgccatg ttcgctgcaa
    11221 agcccccttc actggtgaat gcagcgaact acagcgatct gaaaaacagt aagaccatta
    11281 ttttggccaa ggatgaatac gatgtcttcg gcgacggcaa ggtcatcatc aaatccgcgc
    11341 ccggccatac gcccggccat caagttctga ttctcaaact ggcgaaaacc gggcctgtga
    11401 tgcttgcggg cgatctttat cactaccctg aggagcgcaa gctgaatcgc gtccccacat
    11461 tcgaatacaa caaggagcaa tccctcgcta gccgtgccat gatcgaggac tactgcaaga
    11521 aaacggggac gcagctctgg atcgagcacg actacgtgca caattcaaag cttaaaaaat
    11581 ccccccagta ttacgattag cggcgagtaa atctaaacgc aatgtcactc agttacttgg
    11641 aaatcagcgt aacgcctccg aagtctgcgc ttcggccagt gcctgatgat tagtttactg
    11701 aggcaactgc tgtgactgga gttgctcgtc ctgcccgccg gattttttta aaactaaagt
    11761 aaccggaaac ttcctggcga acttgctgtt aggctcgacg tcctgcggca agatgacgat
    11821 cgtcatcgtg cgtttgtcag cggagagctt gtatgacgcc acgggctgcc atccagaagg
    11881 ataaaacacc ttatcccctg tatcagttga atgcgggtca tagccaaagt gatgaacgtt
    11941 cccggcataa taaccctggg gatccgatcg tcggccgatc gtcactttag tgaaaacact
    12001 tccaccgacc cggcgaaaca caaacattcc gccagtatcc gcgggaggca tataacactc
    12061 ccattgaatt tccccggaat ccagcatctt gcctctgaaa tcaggctccg gattccttac
    12121 cccatctccg cctatcaaat acccatcgat acccgagtcg gttactacga attgttcttg
    12181 atcgcggcca tgtacttcac cccaccacca atcccaggtc gaagtccatg ttcccacaag
    12241 ccaggcgaac cccttttctt gttccttaat ctcagccttt ttatgatcca accaaccttg
    12301 caaattcagc tgtgccatta aatgttgcag gttcggatca ttactgttcg ctgcacctgc
    12361 tgagagtagt ttttgcccgg cttcgtagcc ttcgatcgga tcgttgaggc tcaatgccga
    12421 tgccgctcgc aactgccaca ccagcatttc atccggatgt tgctccaaaa agggacgact
    12481 ctcgctcatg aacttttgca gcaatgtctt ctgttgactt agatccgtgg tctgctgcgc
    12541 ctgccgcgcc agttcgatca gcgaattgta ttcgacctta tccatcccag taagagcggt
    12601 ggcatttgtg cccggcggca gtgaagcagt gctgccagtt ggctggtcgg cactggcccc
    12661 ggtgtccgcc gactgcgagg acgagacggc cttatccagc agccctttcg cggtgatcaa
    12721 ccgttggtcg gccggatacg ctagcaacaa acctgccgcg agctgctgtg cgtctacgta
    12781 tttacctgca tggatcagat cggaaagttt tttcaatacc tcggctggtg cttgtccgct
    12841 cggggccgga ttggccgttg acgcatcggc gggtgcatac ggcgtcgtct gcgcctgtac
    12901 cgacgggcga actgtcagca ccaggacgaa tccgaaaatg agagacattg aaagtttccc
    12961 catcgttttt ctccagtcga tgcgcgcgag agtgaggtcg cggctttata gtcaagggat
    13021 ctctttaatt agcggccggc aggaccacaa agccgccgct cgccaaatgc tcttcgaaat
    13081 tggatttctc cacataacct gtggaattgt ggtaatccgg cccaagattc gaatccatca
    13141 ccgaatagta gattttcccg tctgcggtcc gatacacgcc cgtaaccaca accttgtgct
    13201 ggactccggt cttgtcgagt tgaatcgtgg tgatcaccgg ctgcttcgta ctggcaattg
    13261 cccgggcata actgtccgcg ggaaccggga caaccttacc gaatttgtcc gccactaata
    13321 attcctcggc ggcgttcagt ccgccgcgac ctccgttttg cggttccgtg acgacgattt
    13381 ccggatttgc gcgttcctcg atcggatcca tccccagatc acggacgatc ttcctgaact
    13441 cggcgttcac ttgtttctct ggaacgtgcg cgccattcgc aattgcgtag agaacgcaat
    13501 catcctgagt ttgccgcccc gctcccatgt aggaagcatt ggccgtgacg gattctgcct
    13561 tgccgatatc tttcaccaac tggccatagt agggcggcag ttccgctgaa tcggcacccg
    13621 ctggccgcaa gga
//
LOCUS       DATNDT010000470         3629 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1833943, whole genome shotgun sequence.
ACCESSION   DATNDT010000470 DATNDT010000000
VERSION     DATNDT010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1833943"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1129)
                     /locus_tag="VN788_15535"
     CDS             complement(<1..1129)
                     /locus_tag="VN788_15535"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HXU21995.1"
                     /translation="MAQSTETEVSPSPPAEEHVRTETHSAEPPPPARPSNYFRQRPRA
                     RMFLIVAIVIILVGGFFAWRYFSSYESTDDAQVDGHLMAVSARISGYISKVNVDDNQF
                     VHKGDVLAEIDPRDYQVAVDQARADVATAQATAHSLGIDVPVTSISTTSQVSTTEADV
                     QNAEAGIGVAMQQADAAQAQLAQAQANNIKAQSDLGRYKQLVDKQEISRQLYDQAVAT
                     AAAESAAVSSAKASVAGSSQQVAQARARLAQAQAAWRSSQTGPQQVASVRARALSAEA
                     TVKAKEAALEAAELNLQYAKVLAPADGVVTKNVEVGMNVQPGQQLASVIPLNDVWITA
                     NFKETQLKYMKPGQRVEIKADANGRTYKGHVDSIAGSSGARL"
     gene            complement(1133..2551)
                     /locus_tag="VN788_15540"
     CDS             complement(1133..2551)
                     /locus_tag="VN788_15540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HXU21996.1"
                     /translation="MRPGSSSANSGTNAIESGSNYNSPTSAPARGPSPQGSFLGSVPG
                     KLEPGVVQLSLQDAISRGLRQNLGLLLAGQDIRSARGERWRQLSALLPNVTTSSYVAD
                     SQVDLAEFGFSFTFPGVTIPRVVGPFSYIDSRASVTQNIFDWKAINETHAASESAKSA
                     QHTYKDARDLVTLAVGYEYLQAIADEARIDTADAQLKTAQSLYDQASDQVKAGTSPAI
                     DGLRAQVEMKMRQQQLIQSKNDFAIQKLTLARVIGFAPGQQFELIDKSPYQPFAGMTI
                     EAALKQAYESRSDFRAATEDVRAAEYSRKAAEAGHLPSLSMSADYGLAGTVPQLATHG
                     VFDVRGTLAIPIFQGGKVHGDVLEADARLEKSREHLEDLRTQIDADVRTALLNVESAE
                     QEVAVAQSNIDLAEQTLAQSRDRFRAGVTDTVEVVQAQEAVASAHENYISSLYRDNYA
                     KISLARALGIAEEGVKEYFKGK"
     gene            complement(2728..>3629)
                     /locus_tag="VN788_15545"
     CDS             complement(2728..>3629)
                     /locus_tag="VN788_15545"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HXU21997.1"
                     /translation="FTPTTTCSLLGRGVKIFLVSSKYAQFLEGPPPRQNFHCNRKQVH
                     QPTLGLALAVAVDFRPIVTYSRICPMVMELPKHPRVAVSRLRQSSPARTGRRERHRAE
                     TRKRLFEAALKLFAERGYLETTVEDITEAADVGKGTFFNYFPTKEHVLATLGAERVAA
                     VEHALEKAKSGPVLPALKELATDLAGQSSSPAALLRAIYAAHASCAPVRAELQKRLSL
                     GRILLAEIFRLGQKSGEIRRDHSAAELARLTQMLLFGLTLAWALHPDSSLHETAEDAW
                     NLFFCNLATGNKRANADPRSART"
BASE COUNT          724 a         1016 c         1090 g          799 t
ORIGIN      
        1 ttagacgcgc accgctcgat ccggcgatgc tatccacgtg gcctttgtag gttcggccgt
       61 ttgcatccgc ttttatctcg acacgctggc cgggtttcat atatttcagt tgcgtttcct
      121 tgaaattcgc ggtgatccac acatcgttta agggaatcac gctggccagt tgctggccgg
      181 gttgaacgtt cattcccact tccacgtttt ttgtgaccac gccgtccgcg ggtgccaaca
      241 ccttagcgta ttggagattg agttcggcag cttcgagggc cgcttccttg gctttgacgg
      301 ttgcctcggc cgaaagggcg cgagcgcgca cggacgcgac ctgttgaggg ccggtctgcg
      361 acgagcgcca cgcggcctga gcctgggcca ggcgcgcgcg agcctgagcc acttgttgcg
      421 aagaacccgc cactgaagcc tttgcggaag agaccgcggc actctctgcc gcggcagtgg
      481 ctacagcttg atcgtaaagt tgtcgagaga tttcctgttt gtcgacgagc tgtttgtaac
      541 gtcccagatc gctctgtgct tttatattgt tcgcttgtgc ctgagcgagc tgcgcctgcg
      601 cggcgtccgc ctgctgcatt gccactccaa ttccggcttc ggcattctga acgtccgcct
      661 cagtggtgga tacctggctt gtggtgctaa tcgaagttac gggcacgtcg attcccaaag
      721 aatgcgccgt agcctgggca gtggcgacgt ccgcccgcgc ttgatccacg gcaacttgat
      781 aatcgcgagg atcgatttcg gcaagcacgt cacccttatg gacgaactgg ttgtcgtcca
      841 cgttcacttt ggagatgtat ccggaaatcc gggcgctcac cgccatcaga tggccgtcaa
      901 cctgcgcatc atccgtggat tcgtaacttg aaaagtagcg ccatgcgaag aagcccccca
      961 ccaggatgat cacgatcgcg acgatcagga acattcgcgc gcgaggacgc tgccgaaagt
     1021 agttggacgg gcgagcgggc ggcggaggct cggccgagtg agtctctgta cgaacgtgct
     1081 cctccgcagg agggctgggt gagacttcag tttcagtgct ttgtgccatg gcttattttc
     1141 ctttgaagta ttccttaact ccctcttcgg cgattcccag agctcgggcg agcgaaatct
     1201 tggcgtagtt gtcccggtac aaactggaga tgtaattttc gtgcgcgctg gcaaccgctt
     1261 cctgcgcctg gactacctca accgtatcgg tgactccggc gcgaaaacgg tcgcgggact
     1321 gcgcgagtgt ttgctcagcc aagtctatgt tgctctgcgc cacggctact tcttgctccg
     1381 cggactcgac attcagcagc gcggtgcgaa cgtcagcgtc aatctgcgtg cgaaggtcct
     1441 cgagatgctc gcggcttttt tccaggcgcg cgtcggcttc aagaacgtcg ccgtgaactt
     1501 tgccgccctg gaaaatggga atggccagcg tgcctcgcac atcaaaaacg ccgtgcgtcg
     1561 ccaactgggg aaccgtccca gccaacccgt aatcggcgct catggatagg gaaggcaaat
     1621 ggccggcttc ggcggccttg cgcgaatatt ccgcggcccg cacgtcttcg gtggcggctc
     1681 tgaaatcgga gcgagactcg taagcctgtt tcagcgcagc ctcgatcgtc atgccggcga
     1741 aaggctggta aggagattta tcgattagtt cgaattgctg ccctggagcg aatccgatta
     1801 ctcgcgcgag cgttaacttc tggatggcga aatcgttttt cgattgaatc aattgctgct
     1861 ggcgcatctt catctccact tgcgcgcgca gcccatcaat tgcgggtgaa gttcccgctt
     1921 tcacttggtc cgaggcctgg tcgtagaggg actgagccgt ctttaactgg gcatccgccg
     1981 tgtcgatgcg ggcttcgtcc gcgattgcct gcaaatactc gtaaccgacg gcgagggtta
     2041 ctaagtcgcg cgcgtcctta tatgtgtgtt gcgcggattt agcgctttcg gatgccgcgt
     2101 gggtctcgtt aatggccttc cagtcaaaaa tattctgcgt taccgaagct cgcgaatcga
     2161 tatacgagaa gggtccgaca acccggggga tggtcactcc aggaaatgtg aaggagaagc
     2221 cgaactccgc aagatcgact tgcgagtcgg cgacataact tgaggtggtc acgtttggca
     2281 atagcgcgct cagttgccgc caacgctctc cgcgggcgct gcgaatatcc tggcccgcca
     2341 aaaggagtcc gaggttttgt ctcaggccgc gcgatatggc gtcctgcagc gaaagctgca
     2401 ccacgccggg ctcaagtttt ccgggaacgc tgcccaagaa cgatccctga ggggaagggc
     2461 cacgggccgg cgcggaagtt ggagaattgt aatttgagcc actctcgatt gcgtttgtgc
     2521 cgctatttgc gctggaggaa cctggcctca tttgcgcttg cgcctgtcgt cccataagcg
     2581 gcggcaaaag ggccatgaac atgaaaagaa ctgcggcccg acacgtcaca acatagcctc
     2641 ccttgggcgg ttgtaccttg gatgaagcca aagacatgaa agactcagca taactatcta
     2701 cgacaaaaat aaaagacggg ccgaaaatca cgtgcgcgca ctacgtggat cagcattcgc
     2761 gcgcttattg ccagtggcaa gattgcaaaa gaaaagattc cacgcatctt ccgcagtttc
     2821 gtgcaacgag gaatcgggat gcagagccca cgcgagggta agcccgaaca aaagcatttg
     2881 cgtcagccgc gcgagttcgg cagccgagtg atctcgccga atctcgccgc ttttctgtcc
     2941 gagcctgaat atttccgcca ggagtatccg gccgagggaa agccttttct gcagttccgc
     3001 gcggaccggc gcacaggatg catgagcggc gtagatagcg cgaagcaggg cagcgggcga
     3061 ggaggattgg cccgcgaggt cggtggccag ttctttcagc gccggaagca ccggacccga
     3121 cttcgctttc tcgagagcgt gctcgaccgc ggccacgcgc tcggcgccca aggtggcgag
     3181 tacgtgctct ttggtcggga aataattgaa gaacgtgcct ttaccgacgt cggcggcctc
     3241 ggtgatgtcc tccaccgtag tttccagata gccgcgctct gcaaagagct tcagagctgc
     3301 ttcaaaaagt cgtttgcggg tttccgcgcg gtgacgctcg cggcgtccgg tccgtgcagg
     3361 cgaggattgc cgcaggcgtg agacggcgac tcgaggatgt ttgggcaatt ccatgaccat
     3421 tggacaaata cgactgtaag tcacaattgg cctaaagtca acagcgacgg ccaacgcgag
     3481 acctagtgtg ggctggtgta cctgcttgcg attgcagtgg aagttctgtc tcggaggcgg
     3541 gccttccaga aactgggcgt acttcgagga tacaaggaat atctttacac cacggcccaa
     3601 aaggctgcaa gtagttgttg gtgtaaaga
//
LOCUS       DATNDT010000471         6359 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3862449, whole genome shotgun sequence.
ACCESSION   DATNDT010000471 DATNDT010000000
VERSION     DATNDT010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6359
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3862449"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VN788_15550"
     CDS             <1..286
                     /locus_tag="VN788_15550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HXU21998.1"
                     /translation="LIDVTRGGPSSFSLGAKGRYPMWDSPMHLLIIAIVVLVLFGGRK
                     IPEVMKGLGQGVREFKDGMRGDPPQSGQTSQPVNPPITSPPVSTPVEEKK"
     gene            350..634
                     /locus_tag="VN788_15555"
     CDS             350..634
                     /locus_tag="VN788_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU21999.1"
                     /translation="MSHTPISMFPLAATPRSDEIQAEEAQLVAAAPGEIALDAVVRAQ
                     SLQPQVVRMIALLQNALFHAAEELAKKDPRNPAVEQARLALQGVAEIFTA"
     gene            complement(717..1319)
                     /locus_tag="VN788_15560"
     CDS             complement(717..1319)
                     /locus_tag="VN788_15560"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011392072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HXU22000.1"
                     /translation="MAMMKIVLASASPRRAEILRNAGIPFEVLAAQVDETRRAGELRG
                     DYVRRLALEKARAAAADANADSGYCLFVGADTVVVAGDEILGKPESPADARRMLRKLG
                     ANVHEVHTGLAVVQRPRTAESVIDEVTRVTFAPLSDEEIENYIATGEPFDKAGAYGIQ
                     GIGGRYVTRIEGCYFNVMGLPLARLWSLLQEFGGKEVAPQ"
     gene            complement(1497..2840)
                     /locus_tag="VN788_15565"
     CDS             complement(1497..2840)
                     /locus_tag="VN788_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU22001.1"
                     /translation="MVESAAPLNRDLEPGVLRSESEKKKAGQRALRAAFMGFFVDMFD
                     VYLPIVALGPAMSYFQPATLSPALQSTLYFIVFALSLVGRPVGATLFGHYGDRFGRKK
                     VTLLSMGGFALVTLLIGLLPGYQTWGIGSIALLVFLRFVDGVFLGGEYTGANPLAMEY
                     APKQSRGMWSAIIQAGFPIAMAAMSLITVGLLRLLPAEGIDSPYVRWGWRIPFFLGAI
                     LAGGIFAYYLRVPESEVWASAEKSPSPLRELFRGDNFRCLAQVFVVMSGIWFTLNAIT
                     SILPGVLVTIRHVSSVVVTNAQIVAYVALTPVFILVGVLGQKFGRRIILTAFGLGACT
                     LGPLLYFVLIRAGYRSAAEVIVLVTGVHLCVTPVWAVLAAYINERFHTSVRASGYGVG
                     YSAATIVPAFSSFYMLGLKGLGVPYAYSEIVILALGGVLLLAGALYGPETRHVDI"
     gene            3332..3922
                     /locus_tag="VN788_15570"
     CDS             3332..3922
                     /locus_tag="VN788_15570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22002.1"
                     /translation="MIETLRRRPQVAAVFCLLAVAITAQAVPTVAAPAERYLHVNVQD
                     PTNGESVNVNVPLAMAEKILPAINNHGLHNGKVTIENAEMKGVDVRALLDAVRTAPDN
                     EFVTVKDNDSDVRVAKANGNIIVHVIGKKNKNQKVDVTVPLKIVDALFDSAKNDELDV
                     AAALRALSDAGDILLVTVQDSSQKVRVWVDSRNTQS"
     gene            3984..4424
                     /locus_tag="VN788_15575"
     CDS             3984..4424
                     /locus_tag="VN788_15575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22003.1"
                     /translation="MILLGKIVLGIASVGVAGAGLLCSEGLVHVKVVEKQPHGFHMNV
                     IAPAMLAPIAVHFVPSRDLVQATQQIQPYMPVIRAALDGLRHSEDVVFVDVQEPGEHV
                     HVSKSGGSIVVDVNDDDAIVHVSTPIRAVSSTFEQLTAVTSNSL"
     gene            complement(4814..>6359)
                     /locus_tag="VN788_15580"
     CDS             complement(4814..>6359)
                     /locus_tag="VN788_15580"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HXU22004.1"
                     /translation="KGRDDASLATRSSTDALAEDFSHSVVPLTSFKLRGPLLEADFGT
                     GFCLDRDCQFIGTNYHVAAVERHLRIKGARVVERYLATGPKDEGASLNHSASVVMPPL
                     RFTPSRDLAIFQLSKPLAGYHGLQFSSDNLRIGQEVDIYAYPKGIIDPVRSLQAFHGR
                     FRGISTTELMVIDYAPNRGERIRPGASGGIVVDNETGSVVGILSGSDPSGPPVAVAVP
                     VESLEEFVKTVNPFLARDLFPIHEGASPERPDFYPRYEPERVGELERRPTELEDVSVL
                     RARAQALAEGMRDFIAVQTYIWGSGSHHVEAADSYEVQVRDGSQKFREYPDGKKWLWM
                     PTIPSGPTAGVTPTDVWSTLPLYVGTKVGVKIREAPESDVDGHRIRIFQYRGSAEDEP
                     CVTKTVLDFGVFSLHKDFDSSPYGEVWTDEHENIVRISVHCEKSGWGWGPGETIVTYG
                     WLAKPGEGPRLVPVTVLYQASKKKKLHWCRSEFVNYREFISRPRLVPGPVPVEIEAVN
                     ERAAAH"
BASE COUNT         1382 a         1991 c         1723 g         1263 t
ORIGIN      
        1 cctgatagac gttacacgcg ggggcccaag ctcgttcagc ttgggcgcga aaggcaggta
       61 ccctatgtgg gacagcccca tgcatttgtt aattattgcg atcgtagttc tcgtcctttt
      121 cggtggacgt aaaattcccg aagtgatgaa gggattggga cagggagttc gtgaattcaa
      181 agacggtatg cgcggcgatc cgccgcagtc gggccaaacg agtcagcccg taaatccgcc
      241 cattaccagt ccgcccgtgt ccacgccggt cgaggaaaag aagtaagctc aagttcgagc
      301 gggcgtcgat ggcttggcaa tcttatcggg gcagcccctt acatctgaca tgagccatac
      361 tcccatcagc atgtttcctc tggcggccac gcctcgctca gacgagatac aagccgagga
      421 ggctcagctg gtcgcagctg ctcccggcga aatcgcactt gatgccgtcg tccgcgctca
      481 gagccttcag ccccaggtcg tcaggatgat cgccctgctg caaaacgctt tgtttcacgc
      541 ggcggaagaa ctcgccaaaa aggatccacg aaatcccgcc gtggaacaag ccaggctcgc
      601 ccttcaagga gtcgccgaga tttttaccgc gtaagtttac gttcgagttt ttactgaagc
      661 cccaacccaa tttgttccca tcgccggcca gcatgccgtc gtttctgttt tgcttgttac
      721 tggggcgcaa cctctttccc gccaaattcc tgcaagagtg accaaagccg tgcgagtggc
      781 aagcccatca cgttgaaata gcatccctcg atccgtgtaa cgtaacgtcc tccgatgccc
      841 tgtattccgt atgcgccggc tttgtcgaat ggctcacccg ttgcgatgta gttttcaatc
      901 tcttcgtcgg aaagtggcgc aaaagtcacg cgcgtcactt cgtcaattac gctttcagcc
      961 gtgcgcggcc gctgcaccac ggccagtcca gtgtgaactt catgaacatt cgccccgagc
     1021 ttccgcagca tccgccgcgc gtctgcgggc gattcaggct taccgagaat ttcgtcgccc
     1081 gccaccacca ctgtatcggc gcccacgaat agacagtagc ccgaatccgc attcgcatcg
     1141 gccgccgcgg cgcgggcctt ctcgagcgcc agccgccgca cataatcccc gcgcaattcg
     1201 ccagcccgtc gcgtttcatc cacctgcgca gccagcacct caaacggaat cccagcattg
     1261 cgcaaaatct ctgcccgccg cggtgacgcc gatgccagca caatcttcat catcgccatt
     1321 cattttgcca tacgcgcttt gccattgtag gggcacgatg catcgtgccc ggcaaacatg
     1381 cttggctacc tgctcgccat ctgccgagcg gcttttgtag cgccgacttg ccctgagcct
     1441 cgaagggcat cctgccggcg tctttccggc gattggcggc acaccctggg gaaatgctag
     1501 atgtcgacgt gtctggtctc aggaccgtac aaagctcccg ccagcaacaa cacaccgccc
     1561 agcgcaagaa tcacaatttc gctatacgcg taaggcactc ccaatccctt tagtccgagc
     1621 atgtaaaacg acgaaaacgc cggaacgata gtcgccgcgc tgtacccaac gccgtatcca
     1681 gacgctcgca ccgatgtatg gaaccgttca ttaatgtagg cagcaagcac ggcccacacc
     1741 ggtgtcacgc agagatggac tcccgtcacc agcacgatca cttcggccgc gctccggtac
     1801 cccgccctta tcagaacaaa gtacaaaagt gggcccaggg tgcatgcccc cagcccgaac
     1861 gccgtcagaa taattcttcg accgaatttt tgtccgagga cgcccaccag aatgaacacc
     1921 ggcgtgagag ccacgtatgc gacgatctgc gcattcgtca ccaccacgct gcttacatgc
     1981 cggatcgtca ccagcactcc cggcaaaata gaagtaatcg cattcaacgt gaaccagatg
     2041 ccgctcatca ccacgaatac ttgcgcgagg caccggaaat tgtcgcctcg aaagagttcc
     2101 ctcaaaggcg atggcgattt ttccgccgaa gcccaaacct ccgactcagg cactcgcaag
     2161 taataagcga agattcctcc tgcaagaatc gctcccagaa aaaacggtat gcgccagccc
     2221 cagcgcacgt atggcgaatc aatcccttcc gcgggcaaaa gtcgcagcag ccccacggta
     2281 attagcgaca tcgccgccat tgcgatcgga aaccccgctt gaatgatcgc ggaccacatt
     2341 ccccgcgatt gcttcggcgc atactccatg gccaacggat tggcccccgt atattcgcct
     2401 cccagaaata cgccgtccac aaaccgcagg aaaacgagca gagcaatgct tccgattccc
     2461 catgtttggt accccggcag caagccgatc aacagcgtga ccagcgcgaa tcctcccata
     2521 gatagaagtg tgactttttt ccgcccaaac cgatcaccgt aatgcccgaa aagcgttgct
     2581 cccaccggcc gtcccaccag cgacaacgcg aatacgatga agtacaaagt tgattgcaaa
     2641 gcggggctca gcgtggccgg ctgaaaatag gacatcgcag gccccagcgc cacgatcggc
     2701 agatacacgt cgaacatgtc aacgaaaaat cccatgaagg cggcgcgcaa cgcccgctgc
     2761 cctgccttct ttttttcgga ttcggagcgc agcacccccg gctctaaatc ccgattcaaa
     2821 ggagctgccg attcaaccat ccgcccgcgc ctttccgccg cctggaatca attcttgcgt
     2881 cgaactgctg actgaatcgt cgaacgcgac cgcctgccag cagcaagcgc gcatcgtaca
     2941 cagcacaagg cacaagggca aacggaaagc ccaacacgca gaggccgagt tccgaacgtt
     3001 gcgagtccgc cgatctatcg cagggcgatt tgcttttgtt ggggcacggc atgccgtgcc
     3061 cggtggcgat acttgcctac acgtccgcca tcgtgcccct tcgaatgcca caccgcgtca
     3121 ttccgcgcgc agtgtcccgc gcctttgctt ttgcgcgaag cgccgggaca cgcagtcgag
     3181 gaatctctct tcgatgtaaa ccgaatccgc ggtgtcactt tcaagagaga accctcgttc
     3241 gcgcgcaggt accgagggga agaccaactg aaacatttcg aaagctcgct ccgtattagc
     3301 tggcaggaag ggctgaggag gcccgcacac catgatcgaa accttgcgaa ggcgaccaca
     3361 agtagcagcg gtgttctgtt tgctcgcggt ggcaataacg gcccaagcgg tccctacagt
     3421 ggcggcccct gcggaaagat acctgcacgt gaacgtccag gatccaacga acggcgagag
     3481 cgtcaatgtt aacgtcccgc tcgcgatggc cgagaagatt ctgccggcca tcaacaacca
     3541 cggcctgcac aacggcaagg tcacgatcga aaatgcggaa atgaagggcg tggacgttcg
     3601 cgctctgctg gatgcggtgc gaaccgcccc agacaacgaa ttcgtcaccg tcaaggataa
     3661 tgactcggat gtccgcgtgg ccaaggccaa tggaaacatc atcgttcatg tgatcggcaa
     3721 gaaaaacaag aaccaaaaag ttgacgtcac cgtccccctc aagatcgtcg atgcgctctt
     3781 tgactccgcg aagaacgatg aattggacgt tgcggctgcg ctgcgggcct tgagcgatgc
     3841 aggcgacatc ttgttggtta ctgtgcagga ctcgagccaa aaggttcgcg tttgggtgga
     3901 ttcgcgcaat acgcagagct gagtccatcc cgaaaagccg gaatagcgcc aaggcgtcga
     3961 agccgcgagc ttggagcgca cgcatgatac tgctcggaaa aattgtactg ggaatagcta
     4021 gcgtcggagt ggccggcgca ggccttctat gcagtgaagg tctcgttcac gtgaaagtgg
     4081 ttgagaagca accgcacggc ttccacatga acgtaattgc tcccgcgatg ctggctccta
     4141 ttgccgtgca tttcgtgcct tcgcgcgatc tggtccaggc aactcagcaa atccagccgt
     4201 acatgccggt gattcgcgcg gcgctggatg gtttgcgcca ttcggaagat gtcgtatttg
     4261 ttgacgtgca ggagcccggc gaacacgtgc atgtctcgaa atcgggcggc tcgatcgttg
     4321 tggacgtcaa cgatgatgac gcgattgtgc acgtctccac tccgattcgc gccgtatcaa
     4381 gcacgttcga acaactcacc gccgtcacat caaactcgct ctagcacgcg gccctaatcg
     4441 ggacgaattg agcgcggggc cttagctttt gtctgtgtag cgccatccct tttagggtgg
     4501 catcttgctc ggcccgtgcg cgcagtagta ggggcacgat atatcgtgcc cggcacggat
     4561 actcggccac acatgccggg tagcgccgtc attccgaacg aagtgtcccg tgcctttgcc
     4621 tttgcgcgaa gcgccgggac actatgaagg gctcccaggt cgacggttag tcgagcctgg
     4681 gagatccgtc gacgaatcct ctcttggatc taatctgcgg caacctgtcg cttccgagag
     4741 aggccgtgca ccagtttttg ggtgtgagct ttggcgcata cgacgaactc caagagccgc
     4801 gcgatttgag ccttcaatgt gcagcagccc gctcgttcac ggcctcgatt tcgacaggga
     4861 ccggccccgg cacaaggcgc ggtcggctga taaactcccg gtagttcaca aactcactgc
     4921 ggcaccagtg cagcttcttt ttcttcgagg cttgatatag gacggtcacc ggaacaagcc
     4981 gcggcccctc gcccggctta gcgagccaac cgtatgtcac gatcgtctcg cccggtcccc
     5041 atccccaccc gctcttttcg caatggaccg atattcgcac aatgttttcg tgctcgtcgg
     5101 tccagacctc gccgtagggg gaagaatcga agtccttgtg aagcgagaac actccgaagt
     5161 cgaggacggt cttggtgacg cagggctcgt cctcggcgct tccgcggtat tgaaagatcc
     5221 ttatgcgatg gccgtctacg tccgactccg gggcctcgcg gattttcaca ccgaccttcg
     5281 ttcccacata caaaggaaga gtggaccaga cgtcggtcgg cgtgactcca gcagtcggcc
     5341 cgctgggaat ggttggcatc cacaaccact tctttccatc gggatactct cggaactttt
     5401 gggagccgtc cctgacctgc acctcgtagg agtccgccgc ctcaacgtgg tgactcccgg
     5461 atccccatat gtacgtctgc acggcgatga aatctcgcat cccctcggcg agggcttggg
     5521 cgcgcgcacg gagaacgctc acgtcctcta attcggtagg gcgacgctcg agctcaccaa
     5581 cccgttccgg ttcgtatctc ggatagaagt cgggtcgctc cggcgaagcg ccttcgtgga
     5641 tgggaaaaag atcgcgtgct agaaatgggt tcaccgtctt cacaaactct tccaacgatt
     5701 caaccggcac tgccacggcg accggcggcc cgctagggtc acttccactg aggatgccca
     5761 cgacgctccc agtctcgtta tcgacaacta ttccgccact cgcacccggg cggatgcgct
     5821 ctccacggtt cggcgcatag tcaatgacca taagctccgt cgtcgagatg cccctgaacc
     5881 tcccatggaa cgcttgcaga cttcggactg ggtcgatgat tcccttcggg taggcgtaaa
     5941 tgtcaacctc ctgcccgatt cgaagattgt cgctgctgaa ctggagaccg tggtagcccg
     6001 caagcggctt cgagagttgg aagatggcaa ggtcgcgact cggagtaaac cgaagcggcg
     6061 gcattacgac ggatgcactg tggttcagtg acgcgccctc gtctttcggt cccgtcgcga
     6121 gatatcgctc gactaccctt gcgcctttta tccgaagatg ccgctccact gcggccacgt
     6181 gatagttcgt cccgatgaac tggcaatcgc ggtccaggca aaagccagtc ccaaaatcag
     6241 cttcgagcaa gggcccgcgc aacttgaacg aggtgagtgg caccacagaa tggctgaaat
     6301 cctctgcgag tgcgtcggtc gaagaacgtg tcgcgagcga cgcgtcatcg cggcctttg
//
LOCUS       DATNDT010000472         6722 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3582247, whole genome shotgun sequence.
ACCESSION   DATNDT010000472 DATNDT010000000
VERSION     DATNDT010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6722
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3582247"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..643)
                     /locus_tag="VN788_15585"
     CDS             complement(<1..643)
                     /locus_tag="VN788_15585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22005.1"
                     /translation="MTKRWLLVAAFAALAVESFAVETKFWEQSDASDFEKGSLERLSL
                     RSDGRLFLAPAVTELFDPSTAYLWSIARDSKGNLYTGGAGTGSDTVKLFRIGPSGHGA
                     AWSELPGLEIHAVAVDRQDRVYAATSPDGKVYRITPVGKPELLYDPHAKYIWAMAFAP
                     DGSLYVATGDPGEIHRVTPDGKGAVFYKTHETHARSLAIDPEGNLIVGTEPNGL"
     gene            complement(661..2523)
                     /locus_tag="VN788_15590"
     CDS             complement(661..2523)
                     /locus_tag="VN788_15590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22006.1"
                     /translation="MHKRAAALALAAIGSSLLFARAAEPARPGIGKPQIFKESDLRPG
                     MKGIAWTVFSGNDPEPVPIEILGIERNMWGPKQDVILARMGGKAERTNVAGGMSGSPV
                     YIDGKLVGAVALRLSVFSPDAICGITPIELMLDVNSYDDTKPADARTPDLKKPVNRAS
                     AQAAGGLLQQVLAANSAPGANFPSLTPIDTPLTFSGFSQNALQQFAPVFQQMGMVAVE
                     GGGSSSLPLDAKPGPDWRTALKPGDAVTGVLVSGDMSVSGMCTVTYNDGQHVLACGHS
                     FFNLGPVDMPMAKGEVITTLASTYQPNKIGNATEIVGALHQDRHSAILGDLGATAEMV
                     PVTVRVRSYDQKNVLRKQHDLRFSVFVQQKWTPYLMMLTLFNSVSSLNDFAEEATYRL
                     SGNIQLDGKQSLTLSTMQAPSELPVPAPMLLAGWWGDKFNRLFLNPVEMPKLKSVNVT
                     VDLLPERRTATIQNAWISNNEVEAGSVVPVKVFLRPYRGERIEREIQVKIPVGLSKGD
                     HTILLSDAETLNRMQDRAETAEHFMDIPQTVSLINQERSNDRLYVSVIQPRPTVYYED
                     KALPSLPASVINVMETGRTASHRLISSPESAMEQASIPFDMAISGSYSLKIRVR"
     gene            complement(2663..4285)
                     /locus_tag="VN788_15595"
     CDS             complement(2663..4285)
                     /locus_tag="VN788_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22007.1"
                     /translation="MANIEESSKFLIGLNMDGAVSAGAYTAGVMDFLIDALECWHKAK
                     DDGIPGIPRHEVEICVMSGASAGGMTSAIAAAALMEEFHPVRSIPLAGQNTANKLHQS
                     WVEKIDISELLSDGDLADPKAGVPSLLNSKPIGAIADEAFYLNPRAAGRRAYVAESLQ
                     LILTVTNLRGVPYSVDQSSNIETQIYYYGDDRRFVVEWPAGAAPQADWKSIGEAAVAT
                     GAFPIALAARKIDRPARDYNQRRWLVYEGDPVQEQEIIIAPKWDLTDNDTYATLNVDG
                     GVTNNSPFELNRRTLAAIAPASPNGRNRRAGNTADRAVITIAPFPTSAVFDPKYDYSP
                     SLLALAGKLLTVLVAQSRFSGENLCLVKDEDVFSRFMIAPKIDQPEGKVLNALSGASL
                     GAFGGFLAREFREDDYQLGRRNCQQFLRKHFVLPKDNPIIARGLPKEPLGTQFQFTED
                     GQEWVSIIPLMPEVAGEIPVRRTKLDPSRIEPIAEAAVERLKRVIQGEFQLHGCWNVL
                     FAMAWPILRRRLKNAMVDTILKNLQEQQLLKT"
     gene            complement(4353..5375)
                     /locus_tag="VN788_15600"
     CDS             complement(4353..5375)
                     /locus_tag="VN788_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HXU22008.1"
                     /translation="MNRRTFLLGIGGASAGCMRGPSRRLNIYNWSDYVAHDTIPNFER
                     EFGVRVRYATYESTEEMLAKALTGNSGWDVVFPSNSYVGPMREMDLLAQLDHRQLPNL
                     VNLDVRFQTPPWDRQIQWSVPYMHGSTGIVYSTKAPRPVGWADLWENKYQRRLTMLDD
                     PAEVLGACLKRQQDSVNSTDPGQLEAAKQLAIEQKPLLRAYINAEVRDQLIAGDVLAA
                     QSWSVTAQQAIEQSDRLAFVYPEEGFPLYCDTAAILRESRRVDLAHQFLNYLLRPHIA
                     AAIALEMRTATVNAAARALLPEKDRNNATLYPGPEILQRGEWFRAMPPAAQRLRDRLW
                     TEIKSA"
     gene            complement(5372..6139)
                     /locus_tag="VN788_15605"
     CDS             complement(5372..6139)
                     /locus_tag="VN788_15605"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU22009.1"
                     /translation="MKRFLSVYAAAVYAFLYLPLFVLAVFSFNSSKFAVWRGFTWHWY
                     LDCFHNQALIEATAHSLIIAICATVFSTVIGTLAGYSLWKRRLPWLTNSLYLTLLTPE
                     IVTGISLLAFFQWIFRFLHWHLGLHTVILAHISFSLAYVVIVILARLRTFDPALEEAA
                     LDLGANEWQVFRYVTVPNLLPGIAAAALLCFTVSFDDYVITSLVAGVDSETLPMVIYA
                     MARKGVNPMVNALSAMITFGLGALILLAGRFQKEESA"
     gene            complement(6136..>6722)
                     /locus_tag="VN788_15610"
     CDS             complement(6136..>6722)
                     /locus_tag="VN788_15610"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU22010.1"
                     /translation="IALYIARAQRRRALLLNLVMLPFWTSFLIRTYAWMFLLRDTGLV
                     NSALESLHLIRNPLPLLFNDGAVILGLVYGYLPFMVLPLYATLEKLDPALIDAAQDLG
                     ATPWVTLWKIVVPLSRSGMLAGGLLVFIPCLGAYLTPDLLGGGKSVMVGNLVQNQFTT
                     ARDWPFGSAVSLLLMAAVLLLVLRILGRREQTLL"
BASE COUNT         1461 a         1961 c         1884 g         1416 t
ORIGIN      
        1 tcaatccatt cggctccgtt ccaacgatca gattgccttc tgggtcgatg gcgagcgagc
       61 gagcatgcgt ttcatgcgtt ttatagaaca cagcgccctt accgtcaggc gtcacgcgat
      121 gaatctcgcc cggatccccc gtggccacgt acaaactgcc gtccggggcg aacgccattg
      181 cccagatgta cttcgcatga ggatcataga gcagttccgg ctttcccacc ggtgtaatgc
      241 gataaacttt gccatctggc gatgtcgcgg cgtagacgcg gtcctgacga tccacggcga
      301 ccgcgtgaat ttccagacct ggcaactcgc tccacgcagc gccgtggcca ctcgggccaa
      361 ttcggaataa cttgacagta tcgctgcccg ttcccgctcc gcctgtgtat agatttccct
      421 tggaatctct cgcgatactc cagagatagg cggtcgatgg gtcgaacaat tccgtgacag
      481 cgggcgccag aaataagcgg ccgtcgctac gcagtgaaag gcgctccagc gatcctttct
      541 caaagtccga tgcgtcggat tgctcccaga atttcgtctc aacggcaaac gattccaccg
      601 cgagcgccgc gaaagcggca acaagtaacc atctcttggt catgaagtcg gattgaacgc
      661 ctatctcaca cggattttga gcgaataact gccgctaatg gccatgtcga acgggatgct
      721 cgcctgttcc atcgcgcttt cgggggagct gataagacga tggctcgccg tgcggcctgt
      781 ctccatcaca ttgatcacgg atgcgggcaa cgatggaaga gctttatctt cgtagtatac
      841 ggtcggtcgc ggctgaatca cggaaacata cagccggtcg ttgctccgct cctgattgat
      901 caaagaaacg gtctgcggaa tatccatgaa atgctcggct gtctcggcgc gatcctgcat
      961 tcggttcaat gtttccgcat cgctcagcag aatcgtgtga tctcctttgg aaagccccac
     1021 cgggattttc acctgaattt cccgttcgat tcgctcgcca cgatagggcc gcagaaacac
     1081 tttcactgga accaccgatc ccgcttccac ctcgttgttg gaaatccagg cgttttggat
     1141 tgtcgctgtg cggcgctccg gcaaaagatc cacggtcacg ttcacactct tcaatttcgg
     1201 catttccacg ggattcagaa acagacggtt gaatttatcg ccccaccagc cggcgagcag
     1261 catgggagct ggcacgggaa gctcgctcgg agcttgcatc gtgctcaacg tcaggctttg
     1321 ttttccgtcc agttgaatat tcccgctcaa acggtaagtc gcttcctccg cgaaatcgtt
     1381 caaactggat acggagttga acagtgtcag catcatcagg tacggcgtcc acttctgctg
     1441 cacgaacacg ctgaagcgca gatcgtgctg cttgcgtaag acgttcttct gatcgtacga
     1501 ccgcacacga acggtcaccg gaaccatttc agcggttgcg ccaagatctc ccagaatcgc
     1561 gctgtgccgg tcttggtgca gcgcgcccac gatttcggtc gcgttgccga ttttattcgg
     1621 ctgataagtg gaggcgagcg tcgtgattac ttcgcctttg gccatcggca tgtccaccgg
     1681 accgagattg aagaaggagt ggccacatgc gagcacgtgc tggccgtcgt tatacgtcac
     1741 cgtgcacata ccgctgacgc tcatgtcgcc gctgacaagc acccccgtta cggcatcgcc
     1801 cggtttgaga gcggttcgcc agtccggtcc cggtttagcg tcgagcggaa ggctggacga
     1861 tcctccacct tcaacggcca ccatgcccat ttgttggaat accggcgcga attgctgaag
     1921 cgcattctga ctgaatcccg aaaacgtgag cggcgtgtcg atcggcgtca gcgatgggaa
     1981 gtttgcgccg ggcgcggagt tcgccgccag cacctgctgc agaagaccgc ccgcggcctg
     2041 ggcgctcgca cgattgacag gcttctttaa gtcgggagtc cgcgcatcgg ctggcttcgt
     2101 gtcgtcgtag ctatttacat cgagcatcag ctcaattggc gtaattccgc agatcgcgtc
     2161 gggagaaaat acgctgaggc gaagggccac cgcgccaacc agtttgccat cgatgtaaac
     2221 ggggctgccg ctcattcctc ccgctacatt cgtgcgctcc gctttcccgc ccatgcgcgc
     2281 gaggattacg tcctgcttcg gcccccacat attccgctcg attccgagaa tctcgatcgg
     2341 aacaggttcc ggatcgttgc ccgaaaatac cgtccaggca atacccttca ttcccggccg
     2401 cagatcggac tctttgaaaa tttgtggttt gccaatcccc ggacgagccg gttcggccgc
     2461 ccgcgcgaaa agaagagaag agccgatcgc cgccaaggcc agcgcggccg ctcgtttgtg
     2521 catgaaacct tccttaaaag cgaacgaaag cgaactcgcc tgtgagcgtt gtcagttccg
     2581 caatgtgctt tcttccatta tcatccactc tctgaagcgc acaagctgcg actagcggag
     2641 catcttcggg tcctgttatg ggttaggtct ttagaagttg ctgctcctga agattcttca
     2701 agattgtgtc caccatggcg ttcttcagcc gccggcgcaa aattggccaa gccatcgcaa
     2761 agagcacgtt ccagcacccg tggagttgga attccccttg aatcacccgc tttaaccgtt
     2821 ccacggcggc ttccgcgatc ggttcgattc gcgatggatc gagtttagtc ctccgcaccg
     2881 gaatctctcc cgcgacttcc ggcatcagcg gaatgatcga tacccattcc tgtccatctt
     2941 cggtgaactg aaattgagtc ccgagcggct ccttcgggag tcctcgcgca atgattggat
     3001 tatccttcgg gagcacgaaa tgctttcgca aaaactgctg gcaattccgg cgcccgagct
     3061 gataatcgtc ctcgcgaaat tcgcgagcga gaaatccgcc gaatgcgcca agagacgctc
     3121 ccgataacgc gttcagcacc ttcccctccg gctgatcgat tttcggggcg atcatgaagc
     3181 ggctaaatac atcctcgtct ttcaccaggc agagattctc tcccgaaaac cgcgattgcg
     3241 caaccaagac tgtaagtagc ttgcccgcca gagccaacag ggatgggctg tagtcatact
     3301 taggatcgaa aactgccgac gtgggaaagg gagcgatagt gattaccgcg cggtccgccg
     3361 tattcccggc gcggcgattc cgtccgtttg ggctcgccgg tgcgattgcc gccaatgtgc
     3421 gccggttgag ttcgaacggc gagttgttgg tcacgccgcc gtccacgttc aatgtcgcgt
     3481 atgtatcgtt gtcagtcagg tcccacttcg gcgcgatgat aatttcctgc tcctgcactg
     3541 ggtcgccttc gtataccagc cagcgccgtt gattgtaatc tcgggccgga cggtcgattt
     3601 tccgcgccgc gagggcgatc ggaaatgctc ctgttgccac cgctgcctcg ccgatgctct
     3661 tccaatccgc ctggggagcc gcgcctgctg gccattccac cacgaaccgc cggtcgtcgc
     3721 cgtagtaata gatctgcgtc tctatgttgc tgctctgatc aaccgaataa ggcacgccgc
     3781 gcagatttgt aaccgtcaga attagctgca aagactcagc cacataagct cgccgcccgg
     3841 ctgcccgcgg gttcagataa aaagcctcgt ccgcaattgc gccgatcggc ttggaattca
     3901 ataacgatgg cacgccggct ttcggatcgg ccaaatcgcc gtcggatagc agttcggaaa
     3961 tatcgatttt ctcgacccag ctttgatgca gcttgttggc ggtgttctgc cccgcgaggg
     4021 ggatcgagcg taccggatgg aattcttcca tgagcgccgc cgccgcgatc gcggaagtca
     4081 tccctccggc cgacgctccg gacatcacgc aaatttccac ttcatgccgg gggatgccag
     4141 gtattccatc gtccttcgcc ttgtgccagc actcgagcgc gtcgatcaga aagtccatta
     4201 ctcccgcggt atatgccccg gctgaaaccg cgccatccat attcaggccg attaaaaact
     4261 tagagctttc ttcaatattt gccatgtctc ctccggtgga gagtgcctat tgtaccgggt
     4321 tcgttgcggg aacgagagaa tcttacgagc gcttacgcgg atttgatctc ggtccagaga
     4381 cggtccctca ggcgctgcgc cgcgggcggc atggcccgga accattcacc acgctggagg
     4441 atttcaggac ccggatatag ggtcgcattg ttacggtctt tttccggcag aagcgcgcgg
     4501 gcggctgcgt ttacagtggc agttcgcatt tcgagcgcga tcgcggccgc tatgtgcggg
     4561 cgtagtaaat agttgaggaa ttgatgcgca agatccacgc gcctgctctc gcgcaagata
     4621 gctgccgtgt cgcaatagag cggaaagcct tcttccgggt aaacgaacgc gagtcgatcg
     4681 ctctgctcga tcgcctgctg agcggtgacg gaccaggatt gcgccgccag cacgtcgccg
     4741 gcaatcagtt ggtcccgcac ttccgcgttg atatacgcgc gcaacagcgg cttctgctcg
     4801 atggcgagct gcttcgccgc ttcaagctgg ccggggtcgg tcgagttgac ggaatcttgc
     4861 tggcgtttca ggcaagcgcc gagcacctca gccggatcgt ccagcatagt caagcggcgc
     4921 tgatatttgt tctcccaaag gtcggcccaa cccaccggcc tcggagcttt agtcgaatag
     4981 acaatgccgg tggatccgtg catgtaagga acggaccatt gaatctggcg atcccagggc
     5041 ggcgtttgaa aacgtacgtc cagattgacc agattcggta actggcgatg gtcgagttgg
     5101 gctaggagat ccatctcccg catcggcccg acgtagctat tcgatgggaa aacgacgtcc
     5161 caacccgagt tgcccgttag cgccttggca agcatctctt cggtgctttc gtatgttgca
     5221 tatcgaactc tgacgccgaa ttcgcgttcg aagttcggga ttgtgtcatg cgcaacataa
     5281 tcggaccaat tgtatatatt gagtcgacgc gaaggtccgc gcatgcaacc cgcgctggcc
     5341 ccgccgatgc ctagcaggaa cgtgcgcctg ttcatgcgga ctcttctttc tgaaagcgcc
     5401 cggcaagaag aatgagggcg cccaggccga aggtgatcat cgccgaaagc gcgttgacca
     5461 tcgggttcac gcccttccgt gccattgcat aaatcaccat cggcagcgtc tcggaatcga
     5521 cgccggccac gagactcgta atcacataat cgtcgaatga gaccgtaaag caaagtagcg
     5581 cggcagcggc aatgcccggc agtagattag ggacggttac ataacggaaa acctgccact
     5641 cattcgcgcc gaggtcgagc gccgcttcct caagcgcggg gtcgaacgtc cggaggcgag
     5701 cgagaatcac aatgacaaca taagcgagcg aaaacgagat gtgcgcgaga atcaccgtgt
     5761 gcaggcccag atgccagtgt agaaaccgga agatccattg gaaaaaagcg aggagcgata
     5821 tgccggttac gatctccggt gtaagtaagg tcagatacaa cgagttagta agccatggta
     5881 agcggcgctt ccagagcgag taaccggcca gtgtgccgat gaccgtggag aaaactgtcg
     5941 cgcaaatagc aataatgagc gaatgggcgg ttgcttcgat cagcgcttgg ttgtggaaac
     6001 aatccagata ccaatgccag gtaaagccgc gccagacagc gaatttcgaa gagttgaagc
     6061 taaagaccgc gagcacaaac agaggcaagt agagaaatgc ataaacggcg gcggcgtaaa
     6121 ctgaaagaaa gcgcttcata gcagagtctg ttcgcgccgg ccaagaatac gtaacacgag
     6181 caataatacg gcggccatta acaggagcga caccgccgaa ccgaagggcc agtcgcgcgc
     6241 cgtcgtgaac tgattctgga ccagattccc caccattacg ctttttccgc cgccgagtaa
     6301 gtcgggcgtg agatacgcgc ccaggcatgg aataaagacc agcaagccac cggcgagcat
     6361 tccgctccgg ctcaatggaa cgacgatctt ccaaagagtc acccagggag tggccccgag
     6421 gtcctgcgcg gcgtcgatca aggctgggtc gagtttctcg agcgtcgcgt agagaggaag
     6481 caccatgaaa ggcaaatagc cgtaaaccag gccgaggatc accgcgccat cattgaacaa
     6541 caacggcaat ggattccgaa tcagatgcaa tgattcgaga gcgctattga caaggcccgt
     6601 gtcccgcagg agaaacatcc acgcataggt gcgaatcaga aagctggtcc agaacggcag
     6661 catcaccagg ttcaatagca atgcgcggcg ccgctgggcg cgggcgatat aaagcgcgat
     6721 ag
//
LOCUS       DATNDT010000473         4128 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2138706, whole genome shotgun sequence.
ACCESSION   DATNDT010000473 DATNDT010000000
VERSION     DATNDT010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4128
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2138706"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VN788_15615"
     CDS             <1..338
                     /locus_tag="VN788_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22011.1"
                     /translation="ENLRARRNEKMLPVVGINVVRNETFDWAVKVSVEAIEEQGILFA
                     SLMSTASRVGYAGVRAACCGWSASRYCRPQGCLIFISTTTRFTVLRKYSLCTYLRELG
                     WPAFWPGLI"
     gene            complement(402..1526)
                     /locus_tag="VN788_15620"
     CDS             complement(402..1526)
                     /locus_tag="VN788_15620"
                     /inference="COORDINATES: protein motif:HMM:NF002326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HXU22012.1"
                     /translation="MPPASYAMDVRRSAGRRYPEPAHPYRNDYARDRDRVVHSRAFRR
                     LEAKTQVFTTRFSDHFRNRLTHTLEVAQIARAVAAALDLNTDLAEALALVHDIGHPPF
                     GHAGERQLDLRMREHGSRFDHNLHALRIVEQFEQRYLDFPGLNLTFEVREGIVKHSRD
                     YSAAEFPELSEYCLDERPPLEAQLIDLVDEIAYNTADLDDGFEARLLTVELLRAEAAP
                     FAAAYEEIERHHPDGAEKLKFNEALKRVLDQLATDLIEHTRGEIGRAGVKTLEDVRRF
                     PRRLAGLSPEGAANSASLKRLLSRRLYSHPAIAEDRDRSVAELDGLFEFFLKHPERMP
                     EQYAEQAGREPCHRVVCDYIAGMTDHFLLRQCQEVLGTSA"
     gene            complement(1540..2259)
                     /locus_tag="VN788_15625"
     CDS             complement(1540..2259)
                     /locus_tag="VN788_15625"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU22013.1"
                     /translation="MTQHITRKELKKDEVRETLTQGAELLLSHEKTTIYLVIVAVVIV
                     LGIFGWRAYAQRQSVRAAAAFDGAMTVYQAPVTSQTQGLPPGQIVFTQDSAKYTEASK
                     KFSAIGKQYPRTRSGRLAKYYAALCAEKLGQNSVAQKDLGDLAGSDGDFAAMARFALA
                     QLDDRIGQSAEAEKLYKQLLGESSDLTPKPMVMLALAQHYGQKNPTEAAKLYAQIQTE
                     YPDTTISDQAGQELAMLPGKS"
     gene            complement(2509..2715)
                     /locus_tag="VN788_15630"
     CDS             complement(2509..2715)
                     /locus_tag="VN788_15630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011960044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HXU22014.1"
                     /translation="MAREQGVVKWFNSSKGYGFIQRQSGEDVFVHFSAIQMDGYKSLN
                     EGQLVEFEVRQGPKGFQAENVTAV"
     gene            3263..>4128
                     /locus_tag="VN788_15635"
     rRNA            3263..>4128
                     /locus_tag="VN788_15635"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          846 a         1217 c         1184 g          881 t
ORIGIN      
        1 ccgagaacct tcgtgccaga aggaatgaga agatgctgcc ggtcgtggga ataaatgtcg
       61 ttcgtaacga gacattcgac tgggccgtta aagtctccgt tgaggcgatt gaggagcagg
      121 gtattttgtt cgcatctctc atgtccacgg ccagtcgagt cgggtacgcc ggcgtccggg
      181 ccgcctgctg tggatggtca gcatcgcgtt attgccggcc gcagggatgt cttatcttca
      241 tctccacaac gacgcgattc acggtgctca ggaaatattc cctgtgtacg tatttgcggg
      301 aattgggctg gcctgcattt tggcctggtc ttatctgatt gcgaaaatct ttggatgagg
      361 cgaattggtg gggagagagc cggcgcgcca cccggctcac gctacgcgga cgtccctaga
      421 acttcctggc actgtcgcag caggaaatgg tccgtcatgc cggcgatgta gtcgcacacc
      481 acgcgatggc acggctcgcg ccccgcttgt tcggcgtatt gctccggcat tcgctccgga
      541 tgcttcagga aaaattcaaa gagcccgtcc agctcggcaa ctgaccggtc gcggtcctcg
      601 gcaatcgcag gatgcgaata gagccgccgc gaaaggagcc gcttcaggct ggcgctgttc
      661 gccgcgcctt ccggtgacag tccggccagc cggcgcggaa atcggcgaac gtcttccagc
      721 gttttcacgc ccgcgcggcc aatttcgcct cgcgtgtgct cgatcaggtc cgtcgccagt
      781 tggtcgagca cgcgcttgag cgcctcgttg aatttcagct tctcggcgcc gtcgggatgg
      841 tggcgctcaa tctcctcgta cgccgcggcg aagggcgccg cttccgcgcg cagcaactcc
      901 accgtcaaga gccgcgcctc gaatccatcg tccagatcgg ccgtgttgta ggcgatctcg
      961 tccaccaaat caataagctg cgcttccagg ggcggccgtt catccaggca atattcggat
     1021 aattccggaa attccgcggc ggaatagtcg cgcgaatgct tcacgatgcc ttcgcggacc
     1081 tcgaacgtca aatttagtcc tggaaaatcc agataccgct gctcgaactg ctctacgatc
     1141 cgcagcgcgt gcagattatg atcgaagcgg ctgccatgct ctcgcatgcg gaggtctagc
     1201 tggcgttccc ccgcatgccc gaagggcgga tgcccgatgt cgtgcaccag ggcgagcgcc
     1261 tccgccaaat ccgtattcag gtccagcgcc gcggccaccg cgcgcgcaat ctgcgccacc
     1321 tcaagcgtgt gcgtcagccg attgcgaaag tgatcggaaa accgtgtcgt gaaaacctgc
     1381 gtcttcgctt cgagcctgcg gaaagcgcgc gaatggacta cgcggtcgcg gtcccgcgcg
     1441 taatcgttgc ggtaggggtg cgccggctcg gggtatcggc ggccggccga ccgccgaaca
     1501 tccatcgcgt aggacgccgg cggcatcttt tcccggcgat caggattttc cgggcagcat
     1561 ggccaattcc tggcctgctt ggtccgagat cgtagtatcg gggtattcag tctgaatttg
     1621 tgcgtagagc ttggcggcct cggtcgggtt tttctgcccg taatgctgcg ccagcgccag
     1681 catcaccatc ggcttgggcg tcagatcgga cgattccccc agcagttgtt tgtacagttt
     1741 ctcggcctct gccgattgcc caatccgatc gtcaagctgc gccagggcga agcgcgccat
     1801 cgcggcgaaa tcgccgtcgc tccccgccag atccccaaga tccttttgcg ccacgctgtt
     1861 ctgcccgagc ttttccgcgc agagcgccgc atagtatttc gccagccggc ccgagcgcgt
     1921 ccgcggatac tgcttcccga tggccgaaaa cttcttcgat gcctcggtgt attttgcgct
     1981 gtcctgcgtg aagacgatct gtcccggcgg caagccctgc gtctgcgaag tcaccggcgc
     2041 ctggtagacg gtcatcgccc catcgaacgc cgccgccgcg cgcaccgatt gccgctgcgc
     2101 gtaggcgcgc cagccgaata ttcccagcac gatcaccacc gccacgatca ccagatagat
     2161 cgtcgtcttc tcatgcgaaa gcagcagttc ggccccttgc gtcagggttt cgcgaacttc
     2221 atccttcttc agttctttgc gtgtaatgtg ctgtgtcacc gctctactcc attgcgaacg
     2281 ttggctttcg atactatcac agcgtcaaac ctgccgcgaa tccatgccca ccgcccattt
     2341 tcattcgcac gggaaagaca caaccgcgca tttcccctgc gaacatagat tggcggaaag
     2401 cgaacgccct cgaagcccgc ggatgctctt gcttgcgata cccgccgcgc aacttttcat
     2461 cccgttgctc gcgaattcgc gcttcggttc ttccgtgccc tgaagcgact aaactgccgt
     2521 cacgttctcg gcttgaaagc ccttgggacc ctggcgcact tcgaactcca caagctggcc
     2581 ctcgttcaag gacttgtagc catccatctg aatcgcggag aagtgaacga acacgtcctc
     2641 tcccgattgc cgctggatga acccataccc cttggacgaa ttaaaccact tcaccacacc
     2701 ctgttctcga gccaatgtag ctgctcctct ttgaatgatc tcccgatgaa gtgtttgtaa
     2761 ggccggctgg cagcgaagga atcccgccta gttgcaggaa acttcatacc ttgaggattg
     2821 ttacaagacc gcatcggcgg cgggattctg gcacaaaccg aaaaccctgt ccagcattcc
     2881 cgcgaggact ttaattcccc atccacccag tctcctaata tacggtaccg ggattagaaa
     2941 tgatcctaac ccctgtattt gcagcgcgaa ttcaattcta atctacggta aacaatcccc
     3001 cgcagcgatt ccaaactccc aaaataaaat cttggaaaaa gcattgacag gtaccaatgt
     3061 catctgtata gttagcgttt gcctgtagtc cttccagctg gcctgggagt acctgctctt
     3121 tgacaatcgg atgatacaag ccagaacccg tttggttttc ggccttattc cgggtgtacc
     3181 ggaatcaggg ccatttgaga aaatcgagcg atttaacagt tcgttccgat cgtggcgcaa
     3241 gccacggtcg taacatcagg tttcaaacga gagtttgatc ctggctcaga gcgaacgctg
     3301 gcggcgtgct taacacatgc aagtcgaacg agtgtagcaa tacattcgtg gcgaacgggt
     3361 gcgtaacacg tggataacct tccttcaggt ggggaataac ccagtgaaaa ttgggctaat
     3421 accgcataac aagacgaggg cacatgcttt tgtcttcaaa ggcgcaagtc gccggaagag
     3481 ggatccgcgg ccgattagct agttggcgag gtaatggctc accaaggcaa tgatcggtag
     3541 ccggactgag aggttggccg gccacactgg aactgagaca cggtccagac tcctacggga
     3601 ggcagcagtg gggaatcttg cgcaatgggc gaaagcctga cgcagcaacg ccgcgtgggg
     3661 gatgaagctt ctcggagtgt aaaccccttt cgacccggaa gaatgcccgc aagggtttga
     3721 cggtacgggt ataagaagcc ccggctaact acgtgccagc agccgcggta atacgtaggg
     3781 ggccagcgtt gctcggaatt actgggcgta aagggtccgt aggcggtgtg gcaagtcggg
     3841 agtgaaatct ctgggcttaa ctcagaggct gcttccgaaa ctgccgtgct agagtgtgag
     3901 agaggcgagt ggaattgcgg gtgtagcggt gaaatgcgta gatatccgca ggaacatccg
     3961 aggcgaaagc ggctcgctgg atcacaactg acgctgaggg acgaaagcta ggggagcaaa
     4021 caggattaga taccctggta gtcctagccc taaaccatca ggacttgggg tttgccctgt
     4081 acggggcaag tcccgtagct aacgcgttaa gtcctgcgcc tggggagt
//
LOCUS       DATNDT010000474         6788 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2139434, whole genome shotgun sequence.
ACCESSION   DATNDT010000474 DATNDT010000000
VERSION     DATNDT010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2139434"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..88
                     /locus_tag="VN788_15640"
     CDS             <1..88
                     /locus_tag="VN788_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HXU22015.1"
                     /translation="QRTHTNARTRKGPRKGAVAAKKKTMARK"
     gene            156..602
                     /gene="rpsK"
                     /locus_tag="VN788_15645"
     CDS             156..602
                     /gene="rpsK"
                     /locus_tag="VN788_15645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HXU22016.1"
                     /translation="MAKEPKDAAGAPAAAAKPTKRKKEFRKKKEKRVVPHGLVCIQAT
                     FNNTIVTLTDPDGRAVSWASAGSVGFKGSRKGTPYAAQQAAVTAAGVARDQYGMKSVE
                     VRVKGPGAGRESAVRALASSGLHINAIKDVTPVPHNGCRPPKRRRV"
     gene            761..1393
                     /gene="rpsD"
                     /locus_tag="VN788_15650"
     CDS             761..1393
                     /gene="rpsD"
                     /locus_tag="VN788_15650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006920491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HXU22017.1"
                     /translation="MARHREAVCRLCRREGQKLFLKGLRCFTEKCAIEKRNFVPGQHG
                     QARRPKMVGYGVQLREKQKVKRTYGLLERQFRSYFEKAERTKGPTGSNLMVMLERRLD
                     NVVYRAGLAGSRAQARQLVLHGHIRVQGHKVNIPSYLVKVGDDVVLKEKMHQNAMVLE
                     ARNLAQSQGVAPWLDIDREGCKARVVALPRREDVQTPPMNEQLIVELYSK"
     gene            1456..2484
                     /locus_tag="VN788_15655"
     CDS             1456..2484
                     /locus_tag="VN788_15655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HXU22018.1"
                     /translation="MWKGFQKPKRLAAEPESLTDKFGRFSAQPFERGWGTTVGNALRR
                     ALLSSIEGAAITAVRIEGVLHEFSPIPGVVEDATDIILNLKQIPLKLNTDQPKTVTLN
                     VDVPGPVTSAKIEEDADIAVLDKNIYIATVSEGGKLEMELRVKNGRGYVAADRNFDED
                     LPIGYIPIDSVHSPVRKVNYAVEAARLGQMTDYEKLTLEVWTNAAITPQDSIGLASKL
                     IKDHMSIFINFEEQPELPEEAVENYADPRLEFLDRSVEELELSVRSYNCLKNANIQNL
                     RELVQKTESEMLKTKNFGRKSLNEIKDILHKMGLSLGMKFDEHGRVIWPPLPSQTAPP
                     PAEETTAL"
     gene            2512..2952
                     /gene="rplQ"
                     /locus_tag="VN788_15660"
     CDS             2512..2952
                     /gene="rplQ"
                     /locus_tag="VN788_15660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HXU22019.1"
                     /translation="MRHLKSGSKLGKQPAHRRAVLRNLVTNLIEKERIHTTLLRAKTA
                     RPLAERMITLGKRDSLHTRRQAAAFLMTPVATKKLFTDLAPRFADRPGGYTRIVRTGW
                     RIGDGAELAILEFIGSELKKKVKKGKRAAAKEEPEEKEEAAAEK"
     gene            3574..5982
                     /locus_tag="VN788_15665"
     CDS             3574..5982
                     /locus_tag="VN788_15665"
                     /inference="COORDINATES: protein motif:HMM:NF042645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6600 domain-containing protein"
                     /protein_id="HXU22020.1"
                     /translation="MKKSFAALAVILMLSVVVPAFAQDQAPPPPDQNQQGQYQQNDQN
                     EYPGWETNQPPDQQSNQQADQDQSDQSQSDQAQPGMARLSYLHGDVSSQRGDNGDWVA
                     ATVNTPIAVNDRVSTGNNSRAEIQLDYADVLRMSGSATAKIANLTRSGITVQIGQGLV
                     TYSVLKGGEANSEIDTPNSAIHPNGPGDYRILVNSDAETEVIVRSGSADVSTPQGSTH
                     VDEGQMITVAGTDNPEYKTAAAPGRDDWDSWNNDRDRNIESAKSWRHTDRYYTGSQDL
                     DPYGVWSEVPDYGPVWIPQQGPDWAPYRDGRWVYEPYYGWTWVSYEPWGWAPYHYGRW
                     FVYNGSWAWWPGPLLVDPGYYPIWSPAYVSFIGFGGGVGFGFGFGNVGWFPCGPFDPF
                     FPWWGSFGFRSRSFAFRDFDHFRGRDFDRDRFHDRDMDRFRNRFRGGDHDRFSNANRM
                     FNDPRTRSGVSSMSSHDFGRASVSRRQSPISTATLQRSSMVAGRVGIAPSRESFSPSG
                     RAANPASFRNAPPNSQRFFNRGARANAGINGRQSSSFANARGGSSFGAGRNQGSVQRQ
                     SSQAPARESLQNRGGFQSMNSGRANASGAVQSSRPGWRTFTPPSGSSNTRGPAVNSSR
                     GNVGQSESRGSFQGRPSLQGNSQNRGSWQHFTPPSRQSQAEGQAYGRGYSESNRSERG
                     YGRADQPSRGGYSNSDSRPPLNMRQPIVRPRGPDSRGGSSGGRVYSAPRGGYPGGGYG
                     APRPSYSAPRGGYSGGGGSYRGGGGGGGYHGGGGGGGYHGGGGGSHGGGSHGGGGHSG
                     GHGR"
     gene            6089..>6788
                     /locus_tag="VN788_15670"
     CDS             6089..>6788
                     /locus_tag="VN788_15670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22021.1"
                     /translation="MRKISMLVSLTLFIALAFAAFPGLAAAQDDPPGRVARLNFIQGS
                     ISYQVSGDQDWVQADPNRPLTTGDNLWADKDSRGEVHIGSTAIRLSSETGISFLTLDD
                     RTVQLQLAQGTIEVHLRNQSPDDAFEIDTPNLAFTVTQPGEYRVQTDPNGTSTIIVVR
                     EGAGQVIGGGGDTWDLRPGQQYIFNGADQISYDAVAAPSFDDFADWCESRDQRENAAV
                     SARYVSRDVDGYYDL"
BASE COUNT         1428 a         2037 c         1980 g         1343 t
ORIGIN      
        1 ccagcggacg cacaccaacg ctcgcacccg caaaggtccg cgaaagggcg ccgtcgccgc
       61 gaagaagaaa accatggccc gcaagtaacg cgggttatgt aagctcaggt ccgttttttg
      121 gacctgaggt ttttgaaaaa aagagaggaa ctggcgtggc aaaagaaccg aaggatgcag
      181 cgggcgctcc cgcagcggct gcgaagccga cgaagcgcaa aaaggaattt cgcaagaaga
      241 aggaaaagcg tgtggttcct catggcttgg tgtgcatcca ggccacgttt aacaacacga
      301 ttgtcacgct caccgacccc gatggacgcg ccgtcagttg ggcctccgct ggttcggtcg
      361 gattcaaagg ttcgcgcaag ggcacgccct acgccgcgca gcaggcggcc gtcaccgccg
      421 cgggagtggc gcgcgatcag tacggcatga agtccgtcga ggtgcgtgtg aaaggccccg
      481 gagcggggcg cgaatccgct gttcgcgcgc tcgcttcttc aggcttgcac atcaatgcca
      541 tcaaggacgt cacgcccgtg ccccacaacg gctgccgtcc gccgaaacgc cggcgcgtct
      601 aaatcggccg tggaatttat gtagctcagt ccgcattttg gacctgaggc ttttcggccc
      661 caagaatttc aggtggaagg gcggggtttc aaccccgccg gaagtgcgta tcaaatcaaa
      721 aggccttcag gcctggagca ctgtaagaag gagaaaagca ttggcaagac atcgcgaagc
      781 ggtttgccgg ctgtgccgta gggaagggca gaagctcttc ttgaagggcc tccggtgttt
      841 cacggaaaaa tgcgccatcg aaaagcgcaa ttttgttccc ggccagcatg gtcaagctcg
      901 gcgtccgaag atggtcggct acggcgtgca gttgcgcgaa aaacaaaaag tgaagcgcac
      961 ttacgggctt ctggagcgcc agttccgcag ctattttgaa aaggccgagc gcaccaaggg
     1021 accaaccggc tcgaatctga tggtcatgct cgagcggcgg ctcgataacg tcgtctatcg
     1081 cgccggactc gccggctccc gcgcccaggc tcgccagctt gtgcttcacg gccacattcg
     1141 cgtccaggga cacaaagtaa atattcccag ctatctcgtg aaggtgggcg acgatgtggt
     1201 cctgaaggaa aagatgcatc agaacgccat ggtcctcgaa gcccgcaatc tcgcccagag
     1261 ccaaggcgtc gcgccctggc ttgatatcga tcgcgaaggc tgcaaagccc gcgtagtggc
     1321 cttgccgcgc cgcgaagacg tgcaaacgcc tccgatgaac gagcagttga tcgtcgagtt
     1381 gtactcgaag tagcggacaa atttctagat tggttttatt gcccggcgcg atgccggccg
     1441 aaaggaaggt ctgatatgtg gaaaggtttt cagaagccaa agcggttggc ggccgagccg
     1501 gaatcgctca ccgataagtt cggaagattt tcggcgcagc catttgaacg cggatggggc
     1561 accaccgtcg gtaacgccct gcggcgcgcg ctcctcagct ccatcgaagg cgcggccatt
     1621 accgctgtcc gcatcgaagg cgtgctgcac gaattctcgc cgatcccggg cgtggtcgag
     1681 gacgccaccg acatcatcct caatctcaag cagattcctc tgaagctgaa taccgatcag
     1741 cccaagacgg tcacgctcaa tgtcgatgtg cccggcccag tcacctccgc gaaaattgaa
     1801 gaggacgccg atatcgccgt gctcgataag aatatctata tcgccacggt cagcgaaggc
     1861 ggcaagctcg aaatggaatt gcgcgtcaag aatggccgcg gctatgtcgc cgccgaccgc
     1921 aatttcgatg aagacctgcc catcggctac atccccatcg attccgtcca ctcgccggtt
     1981 cgcaaggtga attacgcggt cgaggccgct cgcttgggac agatgaccga ttacgaaaag
     2041 ctcaccttgg aagtttggac caacgccgcc atcactccgc aggattccat cggcctcgcg
     2101 tccaagctca tcaaggacca tatgagcatc ttcatcaatt tcgaggagca gcccgagctt
     2161 cccgaagagg ccgtcgaaaa ttacgccgac ccgcggttgg aatttctcga tcgctcggtc
     2221 gaggaactcg agctctccgt gcgttcctac aattgcttga agaacgcgaa catccagaat
     2281 cttcgcgagc tcgtccagaa gaccgagtcc gagatgctaa aaaccaaaaa tttcgggcgc
     2341 aagtctctca acgagatcaa ggacatcctt cacaagatgg gattgagcct gggcatgaaa
     2401 tttgacgagc atggccgtgt catttggccg ccgctcccta gccagacggc tcctcctccc
     2461 gcggaggaaa ccacagccct ctgatttttt cagaagaagc tggaatgaaa catgagacac
     2521 cttaaatcag gatccaaact cgggaagcag cctgcgcatc gccgtgcggt gcttcgtaat
     2581 ctggtcacta atttgatcga gaaggagcgc atccatacca cgctcctccg cgccaagacc
     2641 gcgcgtccgc ttgccgaacg catgatcacg ctcggcaagc gcgattcgct tcacaccagg
     2701 cgtcaggctg ccgcttttct gatgactccc gtcgccacga agaagctttt cacggatttg
     2761 gcgccgcgct tcgccgaccg gccgggcggt tacaccagaa tcgtccgaac cggttggcga
     2821 attggtgacg gtgctgaact cgctatccta gaattcatcg gctccgagct gaagaaaaaa
     2881 gtaaagaagg gcaaacgagc cgccgccaag gaagaacccg aagaaaaaga agaagccgcc
     2941 gccgagaagt aaattccaaa agctgttttt gccgtcgtag gggcacgata catcgtgccc
     3001 ggcgcacata cttggctact tgttgaccat ctgcttcggt gaaaaaggcc tgctcccggc
     3061 gcagcgggga ctttgtccct tcagaagttg tattcgtagc ggcataacag gacagggtcc
     3121 ctagcgaacg catttctcgt catcggcttt tgtctttggc gtcgtagggg cgtagcgcgc
     3181 tacgcccgcc gcaaatactt ggctccgcgt ttgcaatccg cctccgcgaa tcgggtcgtc
     3241 atcctaagcg gagtgtgccg atttttctct ccgcattcgt cgcgcattct gcgacgaatg
     3301 cccggcacac gcagtcgagg aacctctctc cgaggtaaaa ctcgtgggcg tgccttgcta
     3361 ggaaccgccg agcgccctca ttggctgaaa accaccacct cgcttgagaa ccaccggcct
     3421 agccagtcgt gccgaatcta ccctcctcga tgccctttgc taagcttatg ttcccatggt
     3481 acttacggct ccatctgaac gtcccgtttg gaacccggcc tgcatactca ccatcggagt
     3541 catacctgtg gcgcgccaca ggcagaggtg cccatgaaaa agtctttcgc agccttggca
     3601 gtgatcctga tgttgtcagt agttgtgccg gcgttcgcgc aggaccaagc gcccccgccg
     3661 cccgaccaaa atcagcaggg ccaatatcag cagaatgacc agaatgagta tccgggttgg
     3721 gaaaccaatc agccgccaga ccagcaatcc aatcagcagg ctgatcagga ccaatccgac
     3781 caaagccagt ccgatcaggc tcagcccgga atggcgcgcc tcagctatct tcacggcgat
     3841 gtttcatccc agcgtggcga caatggagac tgggtcgcgg caactgtgaa cacgccgatc
     3901 gcggtaaatg atcgcgtctc cacgggcaac aattctcgcg ccgaaatcca gctcgactat
     3961 gcagacgtct tgcggatgtc cggcagcgcc actgcgaaaa tcgcgaatct aacgcgcagc
     4021 ggtatcaccg tccagatcgg gcagggcctg gtcacttact ccgtcctgaa aggtggtgag
     4081 gcgaattcgg aaatcgatac gccgaattcc gccattcatc caaacggccc cggtgactat
     4141 cgcatcctgg tgaattccga tgcggagacg gaggtgattg tccgcagcgg ctcggccgat
     4201 gtctctaccc cgcagggaag cacgcatgtt gatgaagggc agatgatcac cgttgcgggc
     4261 acggataatc ctgaatacaa aaccgccgcc gctcccgggc gcgacgattg ggattcctgg
     4321 aacaacgatc gcgaccgcaa cattgaatcc gccaaaagct ggcgccacac ggatcgctac
     4381 tacacaggct cgcaagacct cgacccctac ggcgtctggt ccgaagtccc cgattacggc
     4441 ccagtgtgga ttccccagca aggacctgac tgggcgcctt accgcgatgg tcgctgggtt
     4501 tatgaaccgt attacggctg gacctgggtt tcctacgaac catggggatg ggcgccgtat
     4561 cactacggcc gttggtttgt ttacaacggc agttgggcct ggtggcctgg ccccctcctc
     4621 gttgaccccg gctattaccc gatctggtcg ccggcctacg tctctttcat cggctttgga
     4681 ggcggggtcg gttttggttt cggatttgga aacgtagggt ggttcccttg cggcccgttt
     4741 gatccgttct tcccgtggtg gggttcgttc gggttccgct ccaggtcttt cgctttccgc
     4801 gacttcgacc atttccgggg tcgcgacttt gatcgggatc gctttcatga tcgcgatatg
     4861 gatcgcttcc gtaatcggtt ccgcggcggc gatcacgacc gtttttcgaa tgccaaccgc
     4921 atgttcaacg atccgcgcac acgctccggt gtcagctcca tgtcttcgca tgactttggc
     4981 cgggctagcg tttcaaggcg tcagtcgccc ataagcacgg ccactcttca gcggtccagc
     5041 atggtcgcag gcagggtggg cattgcgccg tcccgagaaa gctttagccc gtccgggcgc
     5101 gctgcaaatc ccgccagctt ccgcaacgct ccgccgaatt cgcagcggtt ctttaaccgc
     5161 ggcgcacggg cgaatgccgg cataaacggc aggcagtcct catcatttgc aaatgctcgc
     5221 ggaggatctt ccttcggcgc tggccggaat caaggctcgg ttcaacgcca gtcgtcgcag
     5281 gcacccgcgc gagagagttt gcaaaatcga ggcgggtttc agtcgatgaa ttcgggccgt
     5341 gcgaacgcct caggagcggt gcaatccagc cggccaggat ggcggacatt cactccgccg
     5401 tctggcagtt cgaacactcg cggaccggca gtaaattcat cgcgtggaaa tgttgggcaa
     5461 tctgaatctc gcggttcctt tcaaggcagg ccgtcgctgc aagggaactc tcagaatcgc
     5521 ggctcgtggc agcacttcac cccgccctcg cgtcagtcgc aggcggaagg gcaggcatat
     5581 ggtcgaggct attcagagtc caatcgctcc gaacgtggct acggccgcgc cgaccagccg
     5641 tcccgaggcg gctattcgaa ctccgattct cgcccgccgc ttaatatgag gcagcccatc
     5701 gtgaggcctc gaggcccgga ctctcgcggc gggtcttccg gtggccgcgt ttacagtgcc
     5761 ccgcgcggcg gctaccccgg tggaggatac ggcgcgccgc gtcctagcta cagtgcgccc
     5821 cgcggcggat actctggcgg aggcggcagc taccgtggag gtggcggtgg aggcggctac
     5881 catggtggcg gcggaggcgg tggctatcac ggtggcggtg gcggatcgca cggcggcggt
     5941 tcgcacggag gcggaggcca ctccggagga cacggccgct agcgagtcgt aatgcttgag
     6001 ccgcagggtc ctgccgtttt tggggctgcg atgcatctaa ggagtaggat gtctggaggc
     6061 tgaacactcg gacaagagga gcacaccaat gcgaaagata agcatgctgg tctcactgac
     6121 cctcttcatc gctttggcct tcgctgcttt tcccggcttg gccgccgcgc aagatgatcc
     6181 acccggacgc gtagcgcgtt tgaatttcat ccagggttcc atctcttatc aggtttctgg
     6241 agatcaggat tgggtgcaag ccgatcccaa tcgtccgctt accaccggcg acaatctgtg
     6301 ggcggacaaa gattcgcgcg gcgaagtgca tatcggcagc accgcgattc gtctttccag
     6361 cgagacgggc atctcttttc tcacgctgga tgatcgcacc gttcagctcc agcttgctca
     6421 ggggaccatc gaagttcatc ttcgaaatca atcgcccgac gacgcattcg aaatcgacac
     6481 tcccaatctc gcattcaccg tcacgcaacc cggtgaatat cgagtccaga ccgatcccaa
     6541 cggaacctcc acaatcattg tggtgcgcga aggagccggc caagtaatcg gtggcggagg
     6601 agacacgtgg gatctgcggc cggggcagca gtacatattc aacggcgccg atcagatttc
     6661 ttacgatgca gtcgccgccc cgtccttcga cgatttcgcg gattggtgtg agtctcgcga
     6721 ccagcgcgaa aatgccgcgg tttcggctcg ctacgtttcg cgcgatgttg atggttacta
     6781 cgatctcg
//
LOCUS       DATNDT010000475         4158 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1567500, whole genome shotgun sequence.
ACCESSION   DATNDT010000475 DATNDT010000000
VERSION     DATNDT010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4158
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1567500"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1309
                     /locus_tag="VN788_15675"
     CDS             <1..1309
                     /locus_tag="VN788_15675"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU22022.1"
                     /translation="DQPTPAPVLLLAYYYPPRNTIGAARPSRFARYLRGFGYDCEVVS
                     FGDKTGFNSGTGVHQVAPRDGLGLAFSPSGLLIRAVQKLFSTYNDRLSWISPAIQEGA
                     RVIRKTSAHLMISTSPPVATHLAALRIKQRTGIKWVADFRDPLSDNPFRSPLWNAIAD
                     PAIERLILHEADRVIANTDAVASVWGARYPQWKYKICVIENGFDPDEPLGPAPLPERN
                     YKLLVYAGEIYGSRYPADLFASMLRLLESGRVDSASHRIKLVGPIREESPIFRDPAAR
                     ALIEGGQLSYDGQARPRKEALDEIARADSLLYLDFHSGLQIPAKLFEYLRIGRPILAF
                     TNSGSPAEHILENSGVPFEAVHPAESNECKDEKLLSFLALPSDFHAPSDWFLDRFNGQ
                     HQTQILARLLDELCFDGPLPAFAGPSPDLIRDPHPVPTGKKIP"
     gene            1700..2290
                     /locus_tag="VN788_15680"
     CDS             1700..2290
                     /locus_tag="VN788_15680"
                     /inference="COORDINATES: protein motif:HMM:NF025695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi-alpha family protein"
                     /protein_id="HXU22023.1"
                     /translation="MEATQTEPIQIPDYIALPLIETATLIDDESLQEKWAWLLANAAM
                     PDATLPVSPLFPKILANLSPREAKFLDVIFDYSIAQIAIKIEPLAADIIARHSRMDDE
                     RLTNAVGNSDFRWKSNHEKAFVLDRLFGEGVFRREQAIDPKSYPALAGKLFAEAEIKT
                     PRKLPTDTVVHMQDFYQLTVMGAEFISACRPPRKRI"
     gene            complement(2458..3576)
                     /locus_tag="VN788_15685"
     CDS             complement(2458..3576)
                     /locus_tag="VN788_15685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU22024.1"
                     /translation="MAWRGQLFFMHIFHVFSTFAVGGPQVRTIQMMNAWGGRFRHTIF
                     AMDGRYVAREKIAPGVPVIFGESSERGLRRVREIVRRVRAERPNLLLTYNWGAIEVAL
                     GAQLFTRCPVIHLEDGFGPDEAHGLKKRRVRTRRMVLRGAYATIVPSRTLYEIARNRY
                     RLPERKIRYIPNGADTERFKPGRNLEWRERLGIAPDALVVGTIGALRPEKNLELLVHA
                     FAAAAIPGSALVVIGEGNCRADLEKLADELKLDGRAFFPGGVADPAPCYGAFDIFAMS
                     SKTEQMPMSLLEAMACGLPAICTDVGDSRHILDTPESPAIVPPGDVEAYAAALRTLAE
                     NPDLRARTGEGNYRRCVREYSFSLMLEAHGELYAEAAR"
     gene            complement(3708..>4158)
                     /locus_tag="VN788_15690"
     CDS             complement(3708..>4158)
                     /locus_tag="VN788_15690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22025.1"
                     /translation="WGGGTNPLPFVLVEDVVSAMVAAGNVAGIDGMAFNLAGDQRPTA
                     QAFVAEISRRSYRDFQFYPQSLIKMQAIEIFKWLMKVAARKPGNSFPPYRDLKSRGLV
                     SYLDCSAAKEKLGWRPNTSLERFYVEAIDAHLKPIPPGDLRLTARAH"
BASE COUNT          897 a         1292 c         1116 g          853 t
ORIGIN      
        1 tgaccagccg actccggctc cagttttgct cctcgcctat tattaccctc cccggaacac
       61 gatcggagcg gcacggccct cgcggtttgc gcggtacctt cgtggcttcg gttacgattg
      121 cgaagtcgtt tcgttcggcg ataagactgg tttcaacagc ggcacaggcg tgcatcaggt
      181 tgccccccgc gacggcctcg ggcttgcgtt ctcgccatcg ggacttttga ttcgtgccgt
      241 ccagaagtta ttttcgactt acaacgaccg gctgagttgg atatctccgg ccatccagga
      301 aggggcgcgc gtgattcgga aaacatcggc gcatctgatg atttccactt caccgcccgt
      361 tgccacgcat ctggccgcgt tgaggatcaa gcaacgtaca ggcatcaagt gggttgccga
      421 tttccgcgat ccgctcagtg acaatccctt ccggtcgccg ttatggaatg ctatcgccga
      481 cccggcgatc gaacggctga ttttgcatga ggcggaccgg gtcatcgcca atacggacgc
      541 ggttgcgtcg gtttggggcg cccggtaccc gcaatggaaa tacaaaatct gcgtgatcga
      601 gaacggtttc gatcccgacg agccgctggg tcccgcacct cttccggagc gcaactacaa
      661 actgcttgtc tatgcgggcg agatttatgg aagccgttat ccggcggacc tgtttgcttc
      721 catgctccgg ctgctcgaat cgggacgggt tgattcggcg tcgcaccgca ttaagctggt
      781 cggcccgatt cgcgaggaat cgccaatctt ccgggatccc gccgcacggg cgctaattga
      841 aggcggacag ctttcctacg acggacaggc gcggcctcgt aaggaagcgc tcgatgagat
      901 agccagggcc gacagcctgc tgtacctgga ttttcactcc gggttacaga ttcccgcgaa
      961 gctgttcgag tatctgcgca tcggcaggcc tattctcgcg tttactaatt ccggctcgcc
     1021 agccgagcat attctcgaaa actcgggtgt cccattcgag gccgtgcatc ccgccgaaag
     1081 caacgaatgt aaggatgaaa agctactgtc gtttctcgcg ttgccatcgg attttcacgc
     1141 cccaagcgat tggtttctgg accgcttcaa cggccagcat caaacgcaga ttctcgcccg
     1201 gctactcgat gaactgtgtt ttgacggccc tttgccagct tttgccgggc catctccaga
     1261 tctaattcgc gacccccatc ctgtacctac gggaaaaaag attccttgag ataacgtggt
     1321 taggtgaaac gtcgatgtcc aactccttaa catagtggca atgaatgcag caggaatcgt
     1381 ggcgggatag ccggcccgcg cgattttcct tccacaccac tacatcggca aaggatacgg
     1441 cgtccggctt gctgggaaat acatttagga cgacaattgt ttaggaggct cgcgctgatc
     1501 gatctgacac cgctggcaca cgcgggccga agtactcggg gatgctgcgg gactacgaag
     1561 ttttctcgga aagctactcg gcccggctct tagtgagacg ggcggactga ttgccgatcc
     1621 gatcgccgag tggcgtaagg gcatcagtgc gcgcagaaga gagcgcttcg accacatcac
     1681 tagcagcacg aaagcacacc tggaggctac acagacggaa ccgattcaaa ttccggacta
     1741 cattgcgctt ccgcttatcg aaaccgccac gcttattgac gatgaaagtc tccaggaaaa
     1801 atgggcctgg cttcttgcga acgccgcaat gccggatgcc accttgcccg tttcgccact
     1861 atttcccaag attcttgcca acttatcgcc aagagaagcc aagtttcttg atgtaatatt
     1921 cgattacagc attgcccaaa ttgctatcaa gatcgaacca ctggctgccg acatcatagc
     1981 gcggcacagt cgtatggatg atgagcgact cactaatgcg gtgggcaata gcgattttcg
     2041 ttggaagtcg aatcacgaga aggcttttgt tctggatagg ttgtttgggg aaggtgtttt
     2101 ccgccgcgaa caggccattg atccgaaatc ttatccggcc cttgccggaa aattgttcgc
     2161 cgaagcggaa atcaaaacgc ctcgcaagct gcccacggat accgttgttc atatgcaaga
     2221 tttctatcag ttgaccgtga tgggtgccga gttcatctcg gcttgccgcc caccaagaaa
     2281 acgcatttaa gggatcccaa tataattaat cagaaaacac ggcgttgtag tccacaaaaa
     2341 agcaaattgg gtgctgatta atgacattat cgcgtgatcg cctgaagtcc gcaggatgtg
     2401 agatgagtcg attcgatgag ctaagtaaat ttgtagagca ccgcgatatg aaccgcatca
     2461 ccgcgccgcc tcggcgtata actccccgtg cgcctccagc atcagcgaaa acgaatactc
     2521 tctgacgcac cgccgatagt tcccctcccc cgtccgtgcc cgcaaatccg gattttccgc
     2581 cagcgtccgc agcgccgccg catacgcctc gacatctccc ggaggcacga tcgccgggga
     2641 ttccggcgtg tctaaaatgt gccgggaatc gcccacgtcc gtgcagatcg cgggcagccc
     2701 gcaggccatc gcttcgagca gcgacatcgg catctgctca gttttggatg acatcgcgaa
     2761 gatgtcgaac gctccgtagc acggcgccgg atcggcaacg ccgcctggaa agaacgcgcg
     2821 gccatcgagc ttcagttcgt cagccaattt ttccagatcc gcgcggcaat tcccttcccc
     2881 aatcacgacg agagcgcttc ccggaatcgc ggccgccgcg aaagcatgga ccagcaactc
     2941 caagttcttc tccggccgca gcgcgccaat cgttccaacc accaacgcat ccggcgcaat
     3001 ccccagacgc tcacgccatt ccaaattccg gccaggttta aagcgttccg tgtcagcgcc
     3061 attcggaatg tagcgaatct tgcgctccgg caaacgatac cggtttcgcg caatctcgta
     3121 cagcgtacgt gagggcacaa tcgtcgcgta ggccccgcgc agcaccatac gccgcgtgcg
     3181 cacccggcgt ttcttcaaac catgcgcttc gtccgggccg aatccgtctt cgagatgaat
     3241 caccgggcag cgcgtaaaaa gctgtgcccc gagcgccacc tcaatggcgc cccagttgta
     3301 cgtcaacagc aggttgggcc gctccgcgcg cacccgacgc acaatctcgc gcacgcgccg
     3361 caacccacgc tcggacgatt cgccgaatat taccggcacg cccggcgcga tcttctcgcg
     3421 cgccacatac cggccatcca tcgcgaaaat cgtgtggcga aagcggccgc cccaggcgtt
     3481 catcatctga atggtccgca cctgcggccc accgacggcg aacgtcgaaa acacatgaaa
     3541 gatatgcatg aagaagagct gcccccgcca cgccaagcgg aaacttcatc gtatccgcac
     3601 cttgccttgt aatgtcttat atcgtggccc ggagcgcacc actctggccc gcaggagcgt
     3661 cagaagcccg attccaagcc gcgcgcgcag caagcggttt ggccggttca atgcgctcgc
     3721 gcggtcaggc gcagatcgcc tggcgggatc ggcttcaagt gcgcgtcaat cgcctccacg
     3781 tagaaacgtt ccaggctcgt attcggccgc cagccaagct tttccttcgc cgccgaacaa
     3841 tcgagataag aaaccagccc gcggctcttc aaatcgcgat acggcggaaa cgagttgccc
     3901 ggttttcgag ccgccacctt catcagccat ttgaaaatct cgatagcttg catcttgata
     3961 aggctctgcg gataaaactg gaaatcgcgg tacgaccgcc gcgagatctc ggccacaaac
     4021 gcctgggccg tcggccgttg atcgcccgcg agattgaacg ccattccgtc gatgcccgcc
     4081 acgtttcccg ccgccaccat cgcggaaacc acatcctcca ccagcacgaa cggaagcgga
     4141 ttcgtgccgc cgccccag
//
LOCUS       DATNDT010000476         5307 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_702242, whole genome shotgun sequence.
ACCESSION   DATNDT010000476 DATNDT010000000
VERSION     DATNDT010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5307
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_702242"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="VN788_15695"
     CDS             complement(<1..478)
                     /locus_tag="VN788_15695"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXU22026.1"
                     /translation="MKRTSASFLPPIALDSRGRTPMYRQLYEWFRHAIFTGQLRPGQR
                     VPSTRSLAAELKISRIPVSSAYEQLHGEGYFETFVGSGTRVAGSIPAETLKLAAGKLS
                     DASRQASRSKAPRSISRRVALMRLPPEIWATNLGAFRVSLPALEHFPAGIWSKLVNR"
     gene            591..1226
                     /locus_tag="VN788_15700"
     CDS             591..1226
                     /locus_tag="VN788_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22027.1"
                     /translation="MNASKTIWMCLLAGGLLFSAHVPAGVGQQNSEAAKTTTQQASAE
                     RDGGHDFDPLIGQWKFHLKRLLHPLTGSNEWVEMEGTGACYKVFDGRAQLDTIELDGP
                     TGHIEGLTLRTYDPQSHQWRLYWANSKTGKLDPPQVGEFKNGRGEFYAQDTINGKTIL
                     IRYVWTKLTTNSPHFEQSFSDDGGKTWEVNWISDQTRVNEETGKTRLSSAR"
     gene            complement(1400..3214)
                     /locus_tag="VN788_15705"
     CDS             complement(1400..3214)
                     /locus_tag="VN788_15705"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXU22028.1"
                     /translation="MLDSPLSETETSPRSKLPGKGHFGVALEPEVIKISAYALLVGLI
                     GGLVAEGLLELIYFFTNVCFYGKFSFAITSPAHNHLGLWVIPIPALGGLLVGAMVYYL
                     EPTLKGHGIPEAMEAVLFSHSRMRLRVAFLKPLATALAIGTGGPFGAEGPIIQTGAAF
                     GSLFGQAVGLTPYYRRVLLAAGAAAGMAATFTAPLAGILVAVELLLFEFRARSFIPVS
                     LAAAIATGVRIHFVGWAPLFPTAAYKLTGFNELWLFGLMGILMGIVGIAMIRALFWIE
                     DLFDNLPLKRALIWSPAIGAAILGAIGYFYPQVFGTGYDTIRDMLNNRLTPADMLGVS
                     VAKFWALVISLGSGTTGGVFAPSLIVGGGIGGAYAIACQHFFPHLVSNPAFYALVAMA
                     AVFGGIARAPFTSIVFLFELSHNPNSLLPLIVCVMVADGFVRLFSRDSIMTGKLVKRG
                     LIVLQDYSVPVLMRARIDQVMRKQFSVMEADTELRTILQKATPENVELLPVVEKDGTL
                     LGIVEPHDMLKVDTQDHHFTMRELARQDYVRAYPGDLVDRVNREMLLKNVENVVVVES
                     YASPKPIGIARANDILQLRRWLMEEETREKPEEGNAPA"
     gene            complement(3252..5018)
                     /locus_tag="VN788_15710"
     CDS             complement(3252..5018)
                     /locus_tag="VN788_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HXU22029.1"
                     /translation="MPKSTRWASKYPMLRKYLPLVHEDLGATYSRNLHKWLLIAPIIG
                     VTTGLVVTAVAIVILRKLWPVVFAYYLHHHWAIVPGVVFGFVVTGVIMQFMTPDPDEH
                     STEEIVRSYHNHQGDIDMQPFLPKLFAAVATVGFGGSAALEGPSIYGGGAIGSWLWAK
                     MRRFNLEPRDRRIMLISGAAAGMAAVFRAPLTGLVFALEMPYKDDLAHEALLPSLIAS
                     VVSYVTLAFFLGAKPLFDFNSATSFTGRDLVWCALLGVAIGLIAMTFSITFRRARDFV
                     VKWAVPHWTKLAVGGLLTGLCGLAFVLIFKDSLVPLGPNYEAVGLILDTQHTPFELIV
                     FGFLKLGATIFSLASGGVSAMFVPLFLTGGSFGTAFGQLAVHSSSVELYAAVGMAAFI
                     SAGYKTPLAAAVFVAEATGGHAFIIPALIGSAVAYAVSGDSSVSGDQHLHEGVRVQEL
                     RDIKVNEFMQRNVISVQGSMSLREFADVISPHYRHTNFPVLEGHRIIGMVPSWSLTRI
                     SAGKWARTTVSDVAVRKIMRVPPDCDAMEALRLLLRERVQPMLLVTSTGGKLEGIVTK
                     TDILEALKTRGEEVLQSADETV"
BASE COUNT         1252 a         1428 c         1619 g         1008 t
ORIGIN      
        1 ggcgattcac aagcttcgac cagattcccg ccgggaaatg ctcgagagcc ggaaggctga
       61 cgcgaaatgc gccgagattg gtggcccaaa tttccggagg caatcgcatc agcgccacgc
      121 gccgcgagat gctgcgcgga gctttgctcc gagaggcttg ccgcgacgcg tcagataatt
      181 ttccggcggc cagttttaac gtttcggctg ggatcgagcc ggccacgcgc gtgccagagc
      241 caacgaacgt ttcaaaataa ccttcgccat gcaattgctc atatgcgctc gataccggta
      301 tacgcgagat ttttagttcg gctgcgaggc tccttgttga aggcacgcgt tgtccgggcc
      361 gcaattggcc ggtgaagatg gcatggcgaa accactcata caactggcgg tacatgggag
      421 tgcggccacg agaatccagg gcaatgggcg gcagaaagct tgcggaggtt cgtttcatcg
      481 atgccgattt tggaaaagtg gctatatcgt atcacacgaa agtggttatt tttaggagcc
      541 acgatgcgga ttagcctttt cattgttcca gggcggttaa ggaggacgca atgaacgcat
      601 cgaaaacaat ttggatgtgt cttctggcag gcggcttgct tttctccgca catgttccgg
      661 ccggagtggg gcagcagaat tcagaggcag cgaaaacgac tacgcagcaa gcttccgctg
      721 aacgcgatgg cggacacgat tttgatccgc tgattggtca atggaaattt cacctgaagc
      781 gcctgctgca tccgctaaca ggctcgaatg agtgggttga aatggagggt acgggcgctt
      841 gctacaaggt tttcgacggt cgcgcccagc tggatacgat tgagctcgat ggcccgacgg
      901 ggcatatcga gggcctcaca ctgcgcacgt acgatcctca atcacaccag tggaggcttt
      961 attgggccaa cagcaaaact ggcaagctgg acccacctca ggtgggcgaa ttcaaaaatg
     1021 gacgcggtga gttctacgcc caggatacga tcaatggcaa aaccatcctt attcgatatg
     1081 tttggacgaa gctgaccacg aattcgccgc acttcgaaca gtcgttttcg gatgatggag
     1141 ggaaaacctg ggaggtgaac tggatctcag atcagacgcg cgtgaacgag gagaccggaa
     1201 aaacgcggct cagctcggcg cgctaaataa atagcggcca aagagccagc aagatgcccg
     1261 tcgaggctca gggcaagccg gcgctacaag agccgcccgg cggatagcgc gcaagtagcc
     1321 aagcatgttt ggcgggcccg gcatgccgtg gcgctaccgc tatgagccgg gcagggtgcg
     1381 tcgctgcgat tggagttctt catgcgggcg cattgccctc ctcgggcttt tcgcgggttt
     1441 cttcttccat cagccagcgg cggagttgaa ggatgtcgtt ggcgcgagca atgccaatag
     1501 gctttgggct cgcgtaggat tcgacgacga cgacattttc gacatttttg agcagcattt
     1561 cccggttcac gcggtcaacc aaatcacccg ggtaagcgcg gacataatcc tggcgagcga
     1621 gctctcgcat agtgaagtgg tggtcttgcg tatcgacttt tagcatgtcg tgaggttcga
     1681 caatgcctag gagcgtgcca tctttttcga ccaccggcag aagctcgaca ttttccggcg
     1741 tggctttctg caggatggta cggagttcgg tgtcggcctc catcacagag aattgcttgc
     1801 gcatgacctg gtcgatgcgc gcgcgcatga gcacaggcac ggaataatcc tggagcacaa
     1861 tgaggccccg ctttacgagt ttcccggtca tgatggaatc gcggctgaag agacgcacga
     1921 atccgtcggc aaccataacg cagacgatga gcggaagcag cgaatttgga ttgtggctga
     1981 gctcgaagag aaacacaatg ctggtaaatg gagcgcgcgc aatgcctcca aagactgcag
     2041 ccatggctac gagcgcgtag aaggcgggat tgctgacgag atgcgggaag aaatgctggc
     2101 aggcgatggc gtaggctccg ccgatgccgc ctccgacaat cagcgacgga gcaaagacgc
     2161 cgcccgtggt ccccgagccc agggagataa caagagccca gaacttggct acagatacgc
     2221 cgagcatatc tgccggcgtg agccgattgt tcagcatgtc gcggatggtg tcgtaaccag
     2281 tgccgaaaac ctgcggataa aagtatccaa tcgcacctaa aatggcggcg ccaatggccg
     2341 gcgaccatat gagcgcgcgt ttgagcggga gattatcgaa gagatcctcg atccaaaata
     2401 aggcccggat catggcgatg ccgacgatgc ccatcagaat gcccatgagg ccaaagagcc
     2461 agagctcatt gaagccggtt agcttatagg ccgcagtagg gaatagaggc gcccatccca
     2521 caaagtgaat gcgaactccc gtggcgatgg cggcagcgag agagaccgga atgaatgagc
     2581 gcgcgcgaaa ctcgaatagc aacagttcca cggccaccag aattccggca agaggtgccg
     2641 tgaaggttgc cgccattcct gccgctgcgc cggcggcgag cagaacgcgc cgataatagg
     2701 gagtgagacc gacggcctgt ccaaaaagcg aaccgaacgc agcgccggtc tgaatgatcg
     2761 gaccttccgc gccgaagggg ccgcccgttc cgatggcaag ggcggtggcc agcggcttca
     2821 ggaatgcgac gcgcagccgc atcctgctgt ggctgaacaa caccgcttcc atcgcttccg
     2881 ggatgccgtg acccttgagg gtgggttcca ggtaataaac catcgctccg acgaggaggc
     2941 cgccgagggc gggaatgggt atcacccaaa gcccgagatg attgtgcgcg gggctggtga
     3001 tcgcaaacga gaacttgccg tagaagcaga cgttcgtgaa aaagtaaatc agttcgagca
     3061 ggccctccgc aacgaggcct ccaataaggc ccaccagcag cgcataggcc gagatcttga
     3121 tgacctcggg ctccagggct acgccgaagt ggccttttcc cgggagtttt gatcgtggcg
     3181 aggtctcagt ttcggagagt ggagaatcaa gcattcgtga gctcctacgg gatcatttgt
     3241 cggaaacgca gtcagaccgt ttcatcagcc gactgcaata cctcttcgcc gcgagttttc
     3301 aatgcttcca agatgtctgt cttcgtgacg atgccttcca attttccgcc ggtggaagtc
     3361 accaaaagca tgggttgtac ccgctcccta agcaacaggc gaagggcctc catagcgtcg
     3421 caatccggag ggacacgcat gattttgcgt acggcgacgt ccgacacggt ggtccttgcc
     3481 cattttcccg cggaaatgcg agtaagcgac catgacggaa ccataccgat aatgcggtga
     3541 ccctcgagga cgggaaagtt ggtgtggcgg tagtgaggcg agatgacatc cgcgaattcg
     3601 cgcagactca tggagccctg aactgaaatt acgttccgtt gcatgaattc attgaccttg
     3661 atgtcccgaa gttcctgcac tctgacgcct tcgtgcaaat gctggtcgcc tgagacggac
     3721 gaatcgccgg aaacggcgta tgccacggcc gatccaatga gcgcgggtat gataaaagcg
     3781 tgaccgccgg tagcttccgc gacaaagacg gcggcggcaa gaggcgtctt ataaccggca
     3841 gaaataaacg cggccatgcc aactgccgca tagagttcga cggaggaact gtgaaccgcc
     3901 aattggccga aggcggttcc gaaagatccg ccggtgagga acagcggaac gaacatggcg
     3961 ctgacgccgc ctgaggcgag tgaaaaaata gttgcgccca acttgaggaa gccgaaaacg
     4021 atcaattcaa acggggtgtg ttgcgtgtcg aggattaaac ctaccgcctc atagttcgga
     4081 ccaagaggca cgagcgaatc cttgaagatc agaacaaaag caagcccaca caaaccggtc
     4141 aacaagccgc cgacggcaag tttcgtccag tgcgggacgg cccatttgac tacaaagtcg
     4201 cgggcgcggc ggaaggtaat agaaaacgtc atggctatga gaccgatcgc cacccccagg
     4261 agcgcgcacc aaacgagatc gcgtcccgta aacgacgtgg cgctattaaa gtcgaaaaga
     4321 ggcttcgcgc cgaggaagaa agcgagcgtg acgtaagaga ccaccgatgc gatgagagaa
     4381 ggcaagagcg cttcgtgcgc aaggtcgtct ttgtagggca tttcgagtgc aaaaaccaag
     4441 cccgttagcg gcgcgcgaaa cacggccgcc atccccgccg ctgcaccgct gattagcatg
     4501 atgcgccggt cgcgcggctc caaattgaaa cgccgcatct tggcccacag ccatgagcca
     4561 atcgcgccgc cgccgtagat gctgggtccc tcgagagcgg cgctgcctcc gaatccgacg
     4621 gtggcgacgg ccgcgaaaag tttgggaaga aacggctgca tatcaatatc gccctgatga
     4681 ttgtggtagg aacgaacgat ttcttccgtg gaatgctcgt cgggatcggg cgtcatgaac
     4741 tgcatgatga cgcctgttac cacaaacccg aacactaccc ccggcacgat ggcccaatgg
     4801 tgatggaggt agtaggcgaa tacaacaggc caaagctttc tcaggatgac tatagcgacg
     4861 gcggtgacca ccaagccagt ggtaaccccg atgatggggg cgattagcag ccacttgtgc
     4921 aggtttcgcg agtaggttgc gccgaggtcc tcatgaacga gcggcaagta cttgcggagc
     4981 atcggatact tcgaggccca tctggtgctc ttcggcattt cgtgttttag tttcctcagt
     5041 tgactaattg ttttttggac gacggcggcg gtttacggcg tgcgatcctt cgcacgcatg
     5101 tccgctcgag ttaatgcttc ttgttaaagg tctgtcctcc cgcgtatcga gatcggccat
     5161 gactggcggg cttcaaaagg ccggctcgga ggcgcacgat ttcctgctcg tggagcctgg
     5221 cgagtctgga aaggacagcc tcaccgcgcg acgtcaacga gacaaacacg tagcgtcgat
     5281 cgcgtgtgtc gtgaaccttg cggacca
//
LOCUS       DATNDT010000477         6211 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1395560, whole genome shotgun sequence.
ACCESSION   DATNDT010000477 DATNDT010000000
VERSION     DATNDT010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6211
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395560"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..447)
                     /locus_tag="VN788_15715"
     CDS             complement(<1..447)
                     /locus_tag="VN788_15715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HXU22030.1"
                     /translation="MPVTFSLRKYQLQDFEALYAIDQACYEPAIAYSRPELRDYLRFP
                     GADCLVAEADSQTAGFILTAHDNRWGYIVTIDVLEPYRRHGVGSMMLQEAERKLAVSG
                     VREVALETATNNACAIAFWKKHGYLTDGVKKNYYPGGIDAYSMRKQI"
     gene            complement(555..2279)
                     /locus_tag="VN788_15720"
     CDS             complement(555..2279)
                     /locus_tag="VN788_15720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22031.1"
                     /translation="MRSWKTLSLAVGLIGALAGAAGAQAPAQQAADQPAATSPQASPL
                     TVNQAIDKIIAREHSENATIRRYNPIIETYIQDMKPDKDMGAVPVSDHYYLGQADLSK
                     GVVDESMLSTKKGKLDQLNPLSHLAGFFEASYVPEGFLQMIYLDTNAFDRQHYHFDYV
                     GRQFLGEVRCLVFDLTPLPKSGRGRFKGRIWAEDQDFTIVRFNGVYTPVSGLNGFNLH
                     FDSWRTNLQPGLWLPSFIFSQESNLKDFLGNHVRFKSQTRLWGYNLKNVGREEEFSQL
                     TIESPDAIQDQAPTDQDRSPVEAEREWQHQAELNVLDRLQRVGLLSPPTPVDKVLETV
                     VNNIEVTNNLDIQPEVHCRVLLTSTLESFAIGHTIVLSRGLIDVLPDEASLATMLAQE
                     LADLIVTKPSTDQWGFNDTTNVSTVEALSHFSFRDTPEDMQRAGAKAVELLKKSPYKD
                     KLGSAGLFLKELSAEEKALPALISPHIGNRVFISDQLLNTGPPLAPDKLDQIAALPIG
                     ARLKLDPWTDRVELLKAKPVALLSAREKMPFEVTPFEPFLTRYEKPDSNAAADQTKPD
                     VARQQQQQ"
     gene            complement(2292..3305)
                     /locus_tag="VN788_15725"
     CDS             complement(2292..3305)
                     /locus_tag="VN788_15725"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HXU22032.1"
                     /translation="MIPPKNQTVFRETLLPDGERRWGSFSVGFALECIALVVVVVFPL
                     LMPQKFEAAEHYWVMPLEAPVIQAWKPQPPPKPIVVKRREVVKQLPKPEPVELPKPKI
                     YNPVVTAPVVRQVRKVQTPDMTQVAKVFPDPNPKLGSSAPPTLRKPREAVQTGGFGDP
                     NGVPTNKNTNRNPNIAQVGAWDMPVGPGNGNGTGGAKGARGVVASAGFGNGVAAGSPG
                     GGSHGTVQQGGFDVKAPVTPEVKHTAAPANTRPVEILSVPKPVYTQEGKAKKIEGDVL
                     VQVLFTAGGEVRVERVVRGLGYGLDESAEAAARQIRFKPAEQDGRPVDFAAIAHITFA
                     LAY"
     gene            complement(3754..5256)
                     /locus_tag="VN788_15730"
     CDS             complement(3754..5256)
                     /locus_tag="VN788_15730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HXU22033.1"
                     /translation="MKSSHLHIPRWVATLVVIAAVIAGGILAIGLKNWTGKDVFGAPK
                     LAVNVARDPAPVPLGNFTNGFASVLKPALPAVVNIHSSKVVKSRPQQMMPFFNDPMFR
                     QFFGQQFGPEQPQSEREQSLGSGVIITSDGTIVTNNHVIDGATDIKVDLPDKREFKAK
                     LIGTDAKTDIAVLKIEATGLPTLTLGDSSKLKVGDVIFAIGDPFAVGETATMGIVSAT
                     GRGGNLGIENYEDFIQTDAAINPGNSGGAMIDLHGDLIGINTAILTGGGGGNQGIGFA
                     IPINMARSVMDQIVTHGKVVRGYLGLLPQDVSPTLAKQFNVNRPGGALVGQIEPGTPA
                     AKAGLKRGDVILSLNGQPVNSANDLRLRISQTPPGTTVKLGVSRDGKIQDFNVTLGEL
                     PEKTAQEGSGNESGPGGLEGVDVQNLTPDLAQQLQLPPGTKGVVVSEVDPSSPAATEL
                     NRGDVIQEVNHKPISNVAEYRQAIAAAGNQPVLLLVNRGGGTQFVVIEPH"
     gene            complement(5552..>6211)
                     /locus_tag="VN788_15735"
     CDS             complement(5552..>6211)
                     /locus_tag="VN788_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HXU22034.1"
                     /translation="AVRDIVIGMSDGLTVPFALAAGLTGAAAALSHGNTIIVTAGFAE
                     IAAGSIAMGLGGYLAAKTDSEHYASELDREYLETVELPEIETEEVAKVFRDYGLSEQQ
                     MAPIVATITSNQKRWVDFMMRFELGLEPPDPARAGRSAGTIAASYIVGGLIPLAPYIL
                     MSSIRPALWTSVTVTLLALLIFGAIKAHYTGVKPLRGATQTALVGGLAAAAAFFIARL
                     V"
BASE COUNT         1270 a         1697 c         1841 g         1403 t
ORIGIN      
        1 gatttgctta cgcatagagt aggcgtcgat gccccccgga tagtagtttt ttttcacccc
       61 gtccgtaaga tagccgtgct ttttccagaa ggcaatggcg caggcattgt tggttgccgt
      121 ctcgagcgcc acttcgcgga caccgcttac ggcaagcttt cgctccgctt cctggagcat
      181 catggatccc acgccgtggc ggcggtaggg ttcgaggaca tcgatcgtga cgatgtagcc
      241 ccagcgattg tcgtgggcgg tgaggatgaa tccagccgtc tggctgtctg cttcggctac
      301 taggcagtcg gctcccggaa agcgaagata gtcgcggagt tcgggacggg aataggcaat
      361 tgcgggctcg tagcatgcct ggtcgattgc gtagagcgct tcaaaatcct ggagctgata
      421 ttttcgcagg ctaaatgtca cgggcacgga tgaatgtttg ccccacatca acacgtccgg
      481 agaaagacgc cgtggcggac ggacagcggc acgcggcaga ggtgccggtt ttgttgctgc
      541 tgcggcggcg cagattattg ctgctgttgt tgtcttgcca cgtccggctt ggtctgatct
      601 gctgcggcat tggagtccgg tttttcgtag cgcgttagga aaggctcgaa cggcgtgacc
      661 tcgaaaggca tcttctcacg agcagaaagc agggctaccg gcttggcctt gagcagctca
      721 acgcggtcag tccaaggatc aagcttgagg cgcgctccaa tgggaagagc cgcgatttga
      781 tcgagcttgt caggagcgag cggcggcccg gtattaagca attggtcgga gataaacacg
      841 cggttgccta tgtgcgggct gatcagtgca gggagagcct tctcttccgc agacaattct
      901 ttcaggaaaa gaccggcgct gcccagcttg tccttgtaag gagatttttt gaggagctcg
      961 acggcttttg ctccggcacg ctgcatatct tcaggggtat ccctaaatga gaaatgactc
     1021 agagcctcta cagtggaaac gttggtcgtg tcattgaagc cccactgatc cgtggaaggc
     1081 ttggtgacaa taaggtcagc gagttcctgg gcaagcattg tagctaggct ggcttcgtcg
     1141 ggaagaacgt cgatgaggcc gcgactgagc acgatagtgt ggccgattgc aaacgactcc
     1201 agcgtgctgg tcaggagcac gcgacaatgg acttccggtt ggatgtcgag attgttggtt
     1261 acctcaatat tattcacgac ggtttcgagg accttatcaa caggtgtagg cggcgacagc
     1321 aatccgacgc gctgcaggcg atccagaacg ttcagttccg cttgatgctg ccattccctt
     1381 tcagcctcga cgggagagcg gtcttgatcc gtgggcgcct gatcctgaat ggcatcggga
     1441 gactcgatgg tgagctggct gaattcttcc tcgcggccga cgttcttgag gttatagccc
     1501 cacaagcgcg tctgagattt gaagcgcacg tgattgccga gaaaatcctt caagttggat
     1561 tcctggctga aaatgaaaga gggcagccaa agtccgggct gaagattcgt acgccagctg
     1621 tcgaaatgca aattaaagcc gttgagtccc gagaccggtg tatagacgcc gttgaaacgg
     1681 acgattgtga agtcctgatc ttcggcccat atgcggccct taaagcggcc tctgccggac
     1741 ttggggaggg gcgtgagatc aaaaacgagg caacgcactt cgcctaggaa ttggcggccg
     1801 acgtagtcaa agtgatagtg ctggcgatca aatgcgttcg tatccaggta aatcatttgg
     1861 aggaagcctt cggggacgta ggatgcctca aagaatccgg caaggtgcga aagcggatta
     1921 agctggtcca gcttgccttt tttcgtcgaa agcatggact cgtctacgac gcccttggaa
     1981 agatcggctt gtccgaggta gtagtggtca cttacaggca ccgcgcccat gtccttatcc
     2041 ggcttcatat cctggatata agtttcaatg atcgggttgt agcggcggat ggtcgcgttt
     2101 tcgctgtgct cgcgagcgat gattttgtcg atggcttggt tgacggtaag gggcgatgcc
     2161 tgggggcttg tcgcggcggg ctgatcggcg gcttgctgtg ccggagcctg tgctccagca
     2221 gcgccagcca gcgcgcctat aagacccacg gccagcgaga gggttttcca gctacgcatc
     2281 actttcccct ttcagtacgc caatgcaaat gtgatatgcg cgatcgcggc gaaatccacc
     2341 ggacgcccat cctgctctgc gggcttgaaa cgaatctggc gcgctgccgc ctcggccgat
     2401 tcatccaagc catagccgag acctcgaacc acgcgctcga ctctaacttc accgccggcg
     2461 gtgaagagta cctggacaag cacatcgcct tcgatctttt tggctttgcc ctcttgagta
     2521 taaactggtt tgggcacaga caaaatttca acgggcctgg tgttcgcagg cgcagccgtg
     2581 tgttttactt caggagtaac cggcgccttc acgtcaaatc caccctgctg gactgtccca
     2641 tgacttccgc cgccgggact gccagcggca acgccgtttc cgaatccagc actggccacc
     2701 acgccgcgcg ctcccttggc accgccggtg ccgttgccat ttccggggcc aacgggcata
     2761 tcccaggcac cgacttgggc gatattggga ttgcggttgg tattcttgtt ggtgggcacg
     2821 ccgttaggat caccaaagcc gccggtttgc accgcctcac gcggctttct cagtgtggga
     2881 ggagccgagc tcccaagttt agggttcgga tcgggaaata cttttgcaac ctgagtcata
     2941 tcgggtgttt gaaccttccg aacctgtctt actacgggcg cggtaacgac cggattatat
     3001 atcttcggct tgggaagctc gacaggttcc ggcttcggaa gctgctttac gacctcgcgc
     3061 cgcttcacga ctatgggttt tggcggcggc tgaggcttcc acgcttgaat tacaggcgct
     3121 tctaaaggca tgacccaata gtgctcggcg gcctcgaatt tttgcggcat cagcaaggga
     3181 aataccacca ctactaccag agcgatgcac tcgagggcga agcctacact gaaagagccc
     3241 catcgcctct cgccatcggg caggagcgtc tcgcggaaga cggtttgatt ctttggcggt
     3301 atcatgatgt ttcaccccga agtgactttg tgtcggggag cttcggacag accaatagcc
     3361 cccaatgtac tagtactacc gtggagggct ggggcgaata gaactcacgt accattctaa
     3421 acccgcgcgt gctgaaagcg ataaccgagt tgtgcgatgt gatctgtggc ctcaagttca
     3481 ttggaaatca tctcgaaacg aatgtgagag agttccgtat tgaatacttt ttggggcgcg
     3541 atagatgctt ggccccaccc ttctcattca agattttcaa cctcgcgcgg aagaggtccg
     3601 ccctcccaag ttcgtaatta acgttagacc atgacgcccc ggcatccgcg agagaataca
     3661 gagttctgtg caaaagtctt gccaagtacc ctgatgcacc ggtcccagag caccctgatg
     3721 gagtgccctc ggagggatgc ccacacctga aactcagtgt ggctcaatta ccacgaactg
     3781 tgtgccgccg ccacggttaa caagcaacag gactggttgg ttgcctgccg cagctattgc
     3841 ctgtctatat tctgcgacgt tcgaaatggg cttgtggttg acctcctgga tgacgtcgcc
     3901 acgattcagt tcggtcgccg ctgggctgga ggggtcgacc tcgctaacca ccactccttt
     3961 cgttccgggt ggtaactgca attgttgagc caagtcgggg gtcaggtttt ggacgtcgac
     4021 gccctccagg ccgcctggac cactctcgtt gccgcttccc tcttgggcag tcttttcagg
     4081 caattcgccc agggtcacgt tgaagtcctg gattttgccg tcgcgcgaaa cgcccaactt
     4141 caccgtcgtt ccgggagggg tctgcgagat gcgcaaacga aggtcattag ccgaattgac
     4201 aggctggccg ttgagactga ggatgacgtc ccctcgcttc agaccggcct tagcagcagg
     4261 cgtacccggt tcaatctgac ctacaagcgc tccgccggga cgattgacat tgaattgttt
     4321 tgcgagcgtg ggcgaaacgt cttgcggcaa gagacctaga tagccgcgca caacctttcc
     4381 atgggtcacg atttggtcca tcaccgaacg ggccatgttg ataggaatag cgaagccgat
     4441 accttggttc ccgccgccac cccccgtgag gattgctgtg ttgatgccga tcaggtcgcc
     4501 atgcaaatct atcatggcgc ccccggagtt tcctggattg atggctgcgt cggtctgaat
     4561 aaagtcctca taattctcga tgccgagatt tccaccgcgg ccggtcgcgc tcacgatacc
     4621 catggtcgcg gtttcgccta ccgcgaaagg atctccgatc gcaaaaatca catcgcccac
     4681 tttgagtttt gacgaatcac cgagagtgag agtgggcagc cccgtggcct cgatcttgag
     4741 aacggcaata tcggttttcg catcggtgcc gatgagcttg gctttgaact cgcgcttgtc
     4801 gggcaagtca accttgatat ccgtggcgcc gtcgatcacg tggttattcg taacgatggt
     4861 tccgtctgac gtgatgatta cgccagagcc aaggctctgc tcccgctctg attggggctg
     4921 ctcgggacca aactgctggc caaaaaattg ccgaaacatg ggatcgttaa aaaaaggcat
     4981 catttgttgt ggccgactct tcacaacttt cgagctgtgg atattgacca ccgccggcag
     5041 cgccggcttg agcacggatg cgaacccgtt ggtaaagttt ccgagaggca ccggagccgg
     5101 gtcgcgggcc acgttgacgg caagcttcgg cgcgccgaat acgtccttac ctgtccagtt
     5161 ttttaatcct atcgcgagta ttccgccggc gataacggcc gctatcacta cgagcgtggc
     5221 cacccaacgg ggaatatgca aatgagaact cttcatgaga tttcttcctt tcggagtcgc
     5281 aaacggcgct acgaatcaaa acgtgacggc ctttaccctc gtcatatgga tgcgccgagg
     5341 aggcgaactc gctgctcaga ccttcccttg gctaccttac agccccgggg ggccggcccg
     5401 gtcactatta taatagatgc aggctttgcc agtagggtag cgcggctctt tctgttgcta
     5461 aatcagttcc gagccgtaaa ggttgcgcaa tgcgagggta tctggctcaa agggcgacag
     5521 tttgcaacgg cggcctcgcg cttaggcctt tctaaacgag cctagcgatg aagaaggcag
     5581 cggcggcagc gagtccgcca actagcgcag tctgcgttgc gccgcgcaac ggcttgacgc
     5641 cggtgtaatg tgctttgatt gcgccgaaga ttagaagcgc gagcaaagtc acagttaccg
     5701 aggtccagag cgcgggacgg atgcttgaca tcaggatgta aggcgccaag gggatcaagc
     5761 ctccgacgat gtaggatgcc gcgatggttc cggcgctccg accggcgcgc gcagggtccg
     5821 gtggctcgag tccgagttcg aagcgcatca tgaaatcgac ccaccttttc tggttcgaag
     5881 taatggtggc gacgatgggc gccatctgct gttcggagag tccataatcg cgaaagacct
     5941 tcgccacctc ctcggtttcg atttcgggga gttcaactgt ctccaagtat tcgcgatcga
     6001 gctcggaggc gtagtgctct gagtccgtct tcgccgctaa gtatccgccg agtcccatgg
     6061 cgatcgagcc agcggctatt tcggcgaagc cggcggtaac gatgatggtg ttgccgtggg
     6121 agagagccgc agctgcgcca gtgagccctg cggcgagggc gaagggtacg gttagaccgt
     6181 cggacattcc gatcacgatg tcccgcacgg c
//
LOCUS       DATNDT010000478         6934 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1279434, whole genome shotgun sequence.
ACCESSION   DATNDT010000478 DATNDT010000000
VERSION     DATNDT010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1279434"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            11..1732
                     /gene="rpoD"
                     /locus_tag="VN788_15740"
     CDS             11..1732
                     /gene="rpoD"
                     /locus_tag="VN788_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HXU22035.1"
                     /translation="MALEEKYDQVRQLIAMGKERGYLLYDEVNDILPAEVHSSEEIDD
                     LLSTFERYGIDVYEDLSSAKAALAAADSTDSAEVVVVKDDAASEDGDLDLTPGTLEKT
                     NDPVRMYLREMGTVPLLTREGEVAIAKRIERGQLLVLKTITRAPLILKELLQVGEDLR
                     NGARSIKEIVQFDDEELTEEKIEAKTRETLKQIDKIAKLYLQAMKLAARLERVPRSKK
                     RPYLHARYAVSRTRVEMSGLVREIDFHPFEKKRLIDKIHQTVERVHFLEREIAKLERR
                     VDASKADVQAEARKDLRARRQELGEIEIASEVSPSELKRALQVILRGEAEAEQAKKEL
                     IEANLRLVVSIAKKYTNRGLQFLDLIQEGNIGLMKAVDKFEWRRGYKFSTYATWWIRQ
                     AITRAIADQARTIRIPVHMIETINKLVRTQRQLVQELGREPTSEEIAKRMDIPVAKVR
                     KILKIAQEPISLETPIGEEEDSHLGDFIEDKAVVSPSDAVINLSLKEQTSSVLKTLTP
                     REEKVIKMRFGLDDGSEHTLEEVGQSFAVTRERIRQIEAKALRKLRHPSRSRKLRAFL
                     ESSANDY"
     gene            1970..2998
                     /locus_tag="VN788_15745"
     CDS             1970..2998
                     /locus_tag="VN788_15745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007761957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU22036.1"
                     /translation="MAIRLDVRGMRVELPSRTGWVAPVNGVSFRLDEGETLGIVGESG
                     SGKTMLALALMGLEPPGARRSGEAWLRQSSRNANGSRLHRTGHPDQNDIANILSATER
                     EMRALRGREIAMIFQEPMTALNPVIRVGSQIEEAIRVHEPRLSAGETRSRALQAMERA
                     AIPEPAMRARQYPHQLSGGLRQRVMIAIALAAGPQLLIADEPTTALDVTVQQKILELL
                     ETLHHDLALSLLFITHDLGVVARIADRIAVMYAGRIVEQGPALEVLRNPRHPYAEALL
                     RAAPSLARQKFTSIPGTVPSLDALPSGCAFAPRCPHATNECEAALPQLLSVGDSSKNH
                     QARCLLAR"
     gene            2988..3827
                     /locus_tag="VN788_15750"
     CDS             2988..3827
                     /locus_tag="VN788_15750"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HXU22037.1"
                     /translation="MPADQAIGREKILEVRDLRKSFRLSVGTFNPAVDVAEDVAPRRL
                     AVDGVTFSLARGETLGIVGESGCGKTTLARMLLRLIEPDSGSIQLWGEDWLGARGAQL
                     RRLRRRIQMIFQDPVASLNPRMRIAAIVGEPLAIHEPALSASERHQRVIHMLEEVGIG
                     EDSLARYPHEFSGGQRQRIGIARALILHPELVIADEPVSALDVSVGAQILELLDRLRR
                     EFSLALILISHSLPVVAQLATRIAVMQGGRFVEMGAAEQVLDAPQQDYTRALLAAVPR
                     LPA"
     gene            4029..4298
                     /locus_tag="VN788_15755"
     CDS             4029..4298
                     /locus_tag="VN788_15755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22038.1"
                     /translation="MDTFQLLLIAWGTITALLICIMIYRGTLNIHEEEHIALGAGGQT
                     LAEEQRAIVSRIEKFSLVSLVLGILSGVLLLIIAGVWLWQNFQSF"
     gene            complement(4318..4728)
                     /locus_tag="VN788_15760"
     CDS             complement(4318..4728)
                     /locus_tag="VN788_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU22039.1"
                     /translation="MAAPIKPPFTLESAKAKVQMAQDAWNSRDPERVALAYTEDSDWR
                     NRDEFFRGREAIKAFLRRKWAKELDYRLRKEMWCWTENRIAVTFQYEWHNPSGHWFRS
                     YGNELWDFDDEGLMRRRIASINDVAIAESERQIR"
     gene            complement(4798..5508)
                     /locus_tag="VN788_15765"
     CDS             complement(4798..5508)
                     /locus_tag="VN788_15765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007904266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU22040.1"
                     /translation="MNSASILIVDDEPQIRRVLRTTLSSQGYTVAEARSGDEALDQIR
                     GQRPDLILLDVNMTSRSGFEVCREIRGSSDIPIIMLTVRNSERDKVQALDAGADDYVV
                     KPFGSEELMARIRAALRRAAPAESLPPFISNDLKVDFEKRSVLVKGQPVRLTPKEFEL
                     LHHLVTNRGKAQAHRRILQAVWGPDYGEETEYLRVFINQLRKKIEPDPRHPRYLHTEP
                     WVGYRFDPPEEKMRSGGS"
     gene            complement(5505..>6934)
                     /locus_tag="VN788_15770"
     CDS             complement(5505..>6934)
                     /locus_tag="VN788_15770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU22041.1"
                     /translation="FLYQRFLPVNATTVALTFLLAILAVSTMWGMVVSVPMSIGAMLA
                     FNYFFLFPAGTLRIADPQNWVALVAFLVVAIIGSHLSSRARQRAADASARQRDIERLY
                     SFSQGLLESRNAMELVNRIPAQVVDAFEVGAAALFVADKQKMYRSGPVIPHLDTERLK
                     AILARDEPLTDAANSVCFVPVRLGVRPIGSIGISGTILPRQTLEAIGTLIAIAMEHAR
                     AVEQVGQTEAAREAEKLRTALLDAVAHALRTPLTSIKVSVTNMLSNPSLSDAQRLELL
                     TIINEESDRLNRLVGEAAEMARLDAGEVELRIEPQPIEHVITAALEQCRGSLGTRDVR
                     VKIVDGLPRARVDVARARQVLTHLIENANQYSPPDQPITITAEASGNFIVTSVADRGS
                     GIDDPEQNLIFEKFYRGKDQRYSVEGTGMGLPIAKAIVEAHGGSISLTSQRGRGSVFS
                     FTLPADYGRQLQSPFQGLSKVHSSQ"
BASE COUNT         1428 a         2145 c         1921 g         1440 t
ORIGIN      
        1 aagcgtgacg ttggctcttg aagagaaata cgaccaggtt cgacagctca tcgccatggg
       61 caaggaacgc ggctacctgc tctacgatga ggtgaacgat atcctgcccg ccgaggtgca
      121 ttcctcggag gaaattgacg acctgctctc gacattcgag cgctatggca tcgatgtgta
      181 tgaagacctt tccagcgcga aggcggcgct ggccgcggcc gacagcacgg actccgccga
      241 agtcgtcgta gttaaggacg acgccgcctc ggaagatggc gacctcgatc tcactccggg
      301 cacgcttgaa aaaaccaacg atcccgttcg catgtatctg cgcgagatgg gcacggtgcc
      361 gctgcttacc cgcgagggtg aagttgcaat cgccaagcgc atcgagcgcg gccaattgct
      421 cgtcctgaag accatcaccc gcgctcccct gatcctaaag gagcttctcc aggtcggcga
      481 ggatttgcgc aacggcgcac gctcgatcaa ggaaatcgtt cagtttgacg acgaggagct
      541 taccgaggaa aagatcgagg ccaaaaccag ggagaccctg aagcagatcg ataagattgc
      601 caagctctac cttcaggcca tgaaactggc cgcgcggctc gagcgagttc ctcggtccaa
      661 gaagcgcccc tacctgcatg cccgttacgc ggtgtcgcga actcgtgtcg aaatgtcggg
      721 tctcgttcgc gagatcgact ttcatccgtt cgaaaaaaag cgcctgatcg acaaaattca
      781 ccagaccgtc gagcgcgtgc actttctcga gcgcgaaatt gccaagctcg aacgccgcgt
      841 cgacgcgtcg aaagccgacg tgcaggccga ggctcgcaaa gaccttcggg cccgccgcca
      901 ggagctgggc gaaatcgaaa tcgccagcga agtcagccct tcggaactca aacgcgccct
      961 tcaggtgatt cttcggggcg aagcggaagc ggagcaagcg aagaaggagc taatcgaagc
     1021 caacctgcgc ctcgttgtct ccatcgccaa gaaatatacc aaccgcggct tgcagttcct
     1081 tgatctgatt caggaaggca acatcggcct gatgaaagcc gtcgataaat tcgaatggcg
     1141 ccgcggctat aaattttcca cgtacgctac ctggtggatt cgccaggcca tcacgcgcgc
     1201 cattgccgac caggcccgca ccatccgaat tcccgtgcac atgatcgaga caatcaacaa
     1261 actcgtgcgc acgcagcgcc agctcgtcca ggaactgggc cgcgaaccca cttccgaaga
     1321 aatcgctaag cggatggata ttcccgtcgc gaaagtccgc aagattctga agattgccca
     1381 ggagccgatc tcgctcgaaa ctccgatcgg cgaagaggaa gattcgcatc tcggcgattt
     1441 catcgaggat aaggccgtag tgtcgccttc cgacgccgtg atcaacctca gcctcaagga
     1501 gcaaacctcc tcggtgctga agactctcac accgcgagag gaaaaggtca tcaagatgcg
     1561 ctttggattg gacgacggca gcgaacacac tctcgaagaa gtcggccagt cgtttgcggt
     1621 cacacgggag cgcatccgac agattgaagc caaggctttg cgtaagctgc gccatccctc
     1681 gcgctcgcgc aagctccgcg ccttcctcga aagctccgcc aacgattact agacgttttt
     1741 cttgacgcgg atgcgggcag aacccgtctc tgctatggag attgagcctc gagaatttct
     1801 tgcagccgct caccacttcg tttctctcgg ctttttcatt cttcaattgt ccgaagattg
     1861 ccactcctcg gaacctaata tatttcgggt gtttcccacg agcctcgcac gaacttagca
     1921 agtgcaaaat tccgcctctc ctcgtatccg ccagacatat gatgtgcgca tggccataag
     1981 gctcgatgta cgcggcatga gagttgaatt gccgagccga acgggctggg tcgccccggt
     2041 gaatggcgtt tcattcaggc tcgacgaggg cgagacgctc ggaatcgttg gtgaatccgg
     2101 ctcgggcaaa accatgctcg cgctcgcgct gatgggcctt gaaccaccag gagcgcgccg
     2161 cagtggtgag gcgtggctgc gtcaaagctc acgcaatgcc aatgggtcgc gcctccaccg
     2221 caccggccat ccggaccaaa atgacatcgc aaacattctt tccgccactg aacgcgagat
     2281 gcgcgccttg cgcgggcgcg aaatcgccat gatttttcag gagccgatga cggcgctcaa
     2341 tcccgtgatt cgcgtgggtt cgcagataga ggaagccatt cgcgttcacg aaccgcgact
     2401 aagcgcaggc gaaactcgca gccgcgctct ccaggcaatg gagcgcgccg cgattccgga
     2461 gccagccatg cgtgcgcgtc aatatccgca ccagctttcc ggcggtctcc ggcagcgcgt
     2521 gatgatcgca atagctctgg ctgccggccc gcagctgtta attgcggacg agcccacaac
     2581 ggcgctcgat gtcaccgtgc aacaaaagat tctcgagctt cttgaaacat tgcaccatga
     2641 tcttgcgctg tcgcttcttt tcatcactca tgacttaggc gtggtagctc gcatcgctga
     2701 tcgtatcgcc gtaatgtacg caggccggat cgtcgagcaa ggtccggcac tcgaagtatt
     2761 gcgcaatcct cgccatccct acgcagaggc actgctacgc gccgcacctt cgcttgcgcg
     2821 ccagaaattc acctcgattc ccggaacggt gccttcgcta gacgctcttc cttccggctg
     2881 cgcgtttgcg ccgcgctgcc ctcacgctac gaacgaatgc gaggccgccc ttcctcagct
     2941 actttccgtc ggggactcct ctaaaaatca ccaagcacgg tgcctccttg cccgctgatc
     3001 aagcgattgg acgtgagaaa attttggaag ttcgcgattt gcgaaagtcg tttcgcctct
     3061 cggttggcac gttcaatcct gctgtcgacg tcgcggagga cgttgccccg cgccgcctcg
     3121 ccgtggatgg cgtcacgttc tcgctcgcgc gtggcgagac gctcggcatc gtcggcgaat
     3181 ccggttgcgg caaaaccact ctcgcccgaa tgctcctacg cctcatcgag ccggattcgg
     3241 ggtccatcca gctgtggggt gaagattggc tgggcgctcg cggtgcgcaa ctgcgcaggc
     3301 tgcggcggcg catccaaatg atcttccagg atccggtcgc ctcgctcaat ccgcgcatgc
     3361 gcatcgccgc catcgttggc gagcctctgg cgattcatga gcccgccctt tcagccagcg
     3421 agcgccatca gcgcgtgatc cacatgctcg aagaagttgg aatcggtgaa gactcgctgg
     3481 cgcgctatcc acacgagttt tccggaggac agcgtcagcg catcggaatc gcccgcgcct
     3541 tgattctcca tcccgaactg gtgatcgcgg atgagccggt ttccgccctg gacgtttctg
     3601 tgggcgcaca gattctggag ctgctagacc ggctgcgccg cgaattctcc ctggcgctta
     3661 ttttgatttc tcacagcttg cccgtcgttg cgcagcttgc cactcgaatc gccgttatgc
     3721 agggcggccg gtttgtcgaa atgggcgctg ccgagcaagt tctcgatgca cctcaacaag
     3781 attacacgcg agcattgctg gccgctgtcc cccgcctccc tgcatgaatg gttaacgaat
     3841 agcctccgac cccaaactta ttgcaattct cacgggacgg tcgtagcttt atatccaggt
     3901 aaaccggcgc aaatccaggg aacttcgact agtggggggg gccgagctcc gttggcaacc
     3961 attgcgacaa agcgtcgcct cgattccttg gccgaagacc gggtcggtta ctgtgtcagg
     4021 aggtcgctat ggacacgttt cagctgctct tgattgcctg gggaactatc accgcgttgc
     4081 tgatctgcat aatgatctat cgcggcaccc tcaacattca cgaagaggaa cacatcgctc
     4141 tcggtgccgg agggcagact ctcgccgagg agcagcgcgc catcgtctcg cgaatcgaaa
     4201 aattcagcct cgtgagcctg gtgctgggca ttctttcagg tgtgttgctt ctgatcattg
     4261 ccggcgtgtg gctctggcag aatttccaga gtttttgatc cgctgccgcc gcgaagatca
     4321 gcgaatctgc cgctcgctct cagctattgc cacgtcgttg atgctcgcga tgcggcgccg
     4381 catcagaccc tcgtcgtcaa agtcccaaag ctcgtttcca tatgagcgaa accagtgccc
     4441 gctcggattg tgccactcgt actgaaaggt gacggcgatg cgattttccg tccagcacca
     4501 catctccttc cgtagccggt aatccagctc cttggcccat tttcgccgca ggaaggcctt
     4561 gatggcctcg cggccgcgga aaaactcatc ccgattccgc caatcggagt cttccgtata
     4621 cgcgagggct acgcgttccg gatcgcgcga attccaggcg tcctgagcca tttgcacttt
     4681 agccttggcg gactccagcg tgaaaggggg cttgattgga gcggccatcg gagattcctc
     4741 caaacgtttc agagttccag cgcaagaata tcgctagtca gtattacggt gcgaccttta
     4801 cgaaccgccg cttcgcatct tttcctcggg cggatcaaag cgatagccaa cccatggctc
     4861 ggtgtgaagg tagcggggat ggcgcgggtc cggctcaatc ttcttcctca attggttgat
     4921 gaatactcgg agatattccg tctcttctcc gtaatcgggg ccccataccg cttgcaaaat
     4981 acgcctatga gcttgcgctt ttcctcggtt ggtcaccagg tggtgcagca gctcaaattc
     5041 cttgggcgtc agtcgcacgg gttgcccctt caccaaaacc gagcgttttt caaaatctac
     5101 cttcaggtca ttggaaataa atggaggcaa cgactccgcc ggggcggcgc gccgcaatgc
     5161 cgcgcggatg cgcgccatca attcttccga tccaaatggc ttcaccacat agtcatccgc
     5221 gccggcatcg agcgcttgca ctttgtcgcg ctcgctgttg cggaccgtca gcatgatgat
     5281 gggaatatcg ctcgatccgc ggatttcgcg gcacacttca aatccggagc ggctggtcat
     5341 attcacgtcg agcaaaatca ggtccggtcg ttggccgcgg atttgatcga gcgcttcatc
     5401 gccgctgcgt gcctcggcga ccgtgtaacc ctgcgagctc aaagttgtgc gcaacacccg
     5461 ccggatttgg ggctcgtcat ccacgatcaa gatgctcgcc gaattcattg gctcgaatgc
     5521 accttcgaga gaccctgaaa gggcgattgc aattggcggc cgtaatcggc cggcaaggta
     5581 aacgaaaata cggagcctcg cccgcgctgg ctcgtgaggc tgatcgaccc gccgtgcgcc
     5641 tcaacaattg ccttcgcaat cggcaaaccc atcccagtcc cctcgacact gtaacgctga
     5701 tccttgccgc gataaaattt ttcgaaaatc aggttctgct ccgggtcgtc gataccgctg
     5761 ccgcggtcag ccacgctggt aacgatgaaa ttccccgatg cctcagccgt aatcgtgatg
     5821 ggctgatcgg gtggtgaata ctgattggca ttttcgatca gatgggtcag aacctgtcgc
     5881 gcgcgggcca cgtccactcg cgcacgcggc agcccgtcca caatcttcac tcgaacgtcc
     5941 cgggtcccaa gagaccccct gcactgctcc agcgccgcag tgattacgtg ctcgatcggt
     6001 tgcggctcga ttctcaactc gacctcaccg gcgtcgagcc gcgccatttc cgccgcctca
     6061 ccgaccaggc gattcaaacg atcgctttcc tcattgatga tagtcagcag ttcaagcctt
     6121 tgcgcgtcgc ttaaggacgg gttggacagc atgttcgtaa cggaaacttt gatcgaggtc
     6181 agcggcgttc gcagtgcatg cgccaccgca tcgagcagcg cggttcgcaa tttttcggcc
     6241 tcgcgcgcgg cttccgtctg gcccacctgc tcgacggctc tcgcgtgctc catggcaatt
     6301 gctatcagtg ttccgatcgc ctcaagcgtt tggcgcggaa ggatcgtgcc tgaaattccg
     6361 atgctgccga ttgggcgcac tcccagccgc accgggacga agcaaacgct atttgcggca
     6421 tccgtcagtg gttcgtcgcg cgccaggatt gctttcaggc gctcggtatc caggtgcgga
     6481 attaccgggc cggagcgata catcttctgt ttatcggcaa caaagagcgc ggctgctccc
     6541 acctcaaagg catccaccac ttgcgcggga atccgattca ccagttccat cgcgttgcgc
     6601 gattcgagca atccttggct gaacgaatac agcctctcaa tatcgcgctg ccgcgctgaa
     6661 gcatccgctg cccgctggcg cgcgcgcgac gagagatggc tcccgataat cgccaccacc
     6721 aggaaggcca ccagcgccac ccaattctgt ggatcggcga ttctgagcgt tcctgcaggg
     6781 aacaaaaaaa agtaattgaa tgccagcatc gccccaatcg acattggcac ggaaacgacc
     6841 atgccccaca tcgtcgaaac cgctaaaatg gctagcagaa acgtgagcgc cacggtcgtg
     6901 gcattcaccg ggaggaacct ctggtacaaa aatg
//
LOCUS       DATNDT010000479         5946 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3305204, whole genome shotgun sequence.
ACCESSION   DATNDT010000479 DATNDT010000000
VERSION     DATNDT010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5946)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5946)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5946
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3305204"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            144..665
                     /locus_tag="VN788_15775"
     CDS             144..665
                     /locus_tag="VN788_15775"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU22042.1"
                     /translation="MKSIQRIGLCLFVLIAVGLGASRLSSRESPKTSPQKAGAQKAAA
                     QKNAPKTPSTAEPAGSVERGQYLVENVAMCEECHTPRDGGGNLDESRRLQGAPIWITP
                     VHHNTNWAMNAPSLAGFEQFTEEQGEQILEHGVGPNGLPIQPPMHIYHMNHADAQAII
                     AYLRSLPGGYPQR"
     gene            712..2328
                     /locus_tag="VN788_15780"
     CDS             712..2328
                     /locus_tag="VN788_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase maturation factor family protein"
                     /protein_id="HXU22043.1"
                     /translation="MEEHLKAWVEWLLGPETSDEKHLGHLWPRWIFLRALGLIYFSAF
                     YSLIFQIKGLLGPKGILPAADYLQAVTAALHSARFWFAPTLFWLSSSDRALMLVCWVG
                     LAASILLVLNVWPRAALAVCFVCFLSFIGAAQDFASYQSDGMLLGAGFVSLFFAPPGF
                     LPGLGRANPPSRASLFLLLWEWFRIYFESGFAKMASGDTSWRNFTAMDDYYQNGPLPT
                     WIGWHVGHLPHWFHASSVFYTLAVELVLVWMVFLPRRFRIACFWIVAPFEISIILTGN
                     YAFLNYLVFSLGFLLLDDRFVEWIVPQRIRGAFERKRDGVPAPEALPDDWRSAWRKRL
                     KPVRMTVAGICLGLVFYATTAIFIWTLVPNVPLPQKPVTMLEPFRIANRYALFANMTH
                     QRYEIEFQGSKDGKTWVPYPFRYKPQDPSKPPGIYAPYQPRFEWNLWFASLGNWREYR
                     FVLWTEERLLTNEPDVLELFARNPFPGTPPKEVRSVRYQYWFTSEETRRKQGLWWRRE
                     MLGEYAPTLEREPDGKIVILDFPGDEAPTR"
     gene            complement(2347..2928)
                     /locus_tag="VN788_15785"
     CDS             complement(2347..2928)
                     /locus_tag="VN788_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22044.1"
                     /translation="MFIAKWAPLILLLSLSGATAARQSQAQTPSASGQQQNSLAAAAR
                     RAREAKKDQAKPPKVWDNESLASLHGDISIVGQPPAPPSNPAPAAADKSAASNPPAAD
                     KSALEADLSAAKANLDKLKVDLDLLQRKYGLDQQTYYGKPEYSSDTAIAAALEDEKNQ
                     VASKQQEVTDAEKKVADLQTQLDAVNKSSNNAK"
     gene            3189..4298
                     /locus_tag="VN788_15790"
     CDS             3189..4298
                     /locus_tag="VN788_15790"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HXU22045.1"
                     /translation="MKTIPALRQAVSILCLTLISVPATAFGKAPARHRHYRHRARVVQ
                     YVNPAKDDIVINDDPVVRNIAVDALGHEKGSVLAVDPTNGRILSIVNQKLAFSAGFEP
                     CSTIKPFVALAALKEGVITRDTMIRVESRRYINLTEAMAHSNNKFFEEVGRRLGFDRE
                     LKYDALLGLGQRVGYGIPEEQPGSLPPSAPLFGGVARMSSFGEGIRITPFQLASLVGM
                     LANGGTQYYLQYPRTEAARQNFEPRIRERFDIAPLLPDLREGMLAAVLYGTAKRSYDP
                     DGEQDLGKTGTCNDEGVGGRLGWFVSYADQAHPKIVIVVLLHGGARIISGPHASEIAG
                     RIYRGLYQRNYFAREDSASRQYASSPAPSGSVPSP"
     gene            4308..5744
                     /locus_tag="VN788_15795"
     CDS             4308..5744
                     /locus_tag="VN788_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008480646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HXU22046.1"
                     /translation="MRPSSVLRKTAYDAVVVGSGPNGLAAAIVMAKADRSVLVLEAED
                     SIGGGTRSAQLTLPGYVHDICSAVHPMALASPFFRSLPLEEHGLEWIYPPLPLAHPRD
                     DGTAMVLDRSLDGTCESLGVDAQSYRNLLGPLVRNWGVVESAILGPLGVPRNPIAMSR
                     FGLHAIQPAARLARNKFQTEAARVLFAGLACHSVVPLEKWGTAAIGLVLAAVAHVYSW
                     PIPKGGSQRIGDALASYLRSLGGEIQCGVRVESIDDIPSARTIFCDLTPQGLLKIAAQ
                     VLPANYQKALRRYKYGPGVFKVDWALSNPIPWKAKECSGAGTVHVGSSLEEIERSERA
                     AWNGSQDDKPFVLVTQPSLFDSTRAPVGRHTAWAYCHVSNNSDCDMTSRIESQIERVA
                     PGFRDAILARSVRGPAELQRHNANLVGGDISGGANNLMQLFFRPTRRTYATPHRGLYL
                     CSSSTPPGGGVHGMCGYHAAVRAIAAGE"
BASE COUNT         1164 a         1655 c         1776 g         1351 t
ORIGIN      
        1 tcgcgcgcga gttagcctga cggaagggaa ctgaaaaacc cgatggacac ggaaacgctc
       61 tttttgggat actttcgcgt gcaagcggcg atgaatgatc aagcgaaacc tgccgcatct
      121 cttaattcag gactaaaaac cttatgaaat caattcaacg gattggtcta tgtttgttcg
      181 tgctgatcgc ggtgggactg ggggcatcgc gcttgtcgtc gcgcgagagt cccaaaacat
      241 ctccgcagaa ggctggtgcg cagaaggccg ctgcgcaaaa gaatgcgcca aagacccctt
      301 ccaccgcgga gcccgctggc agcgtcgaga gagggcagta tttggttgag aacgtcgcaa
      361 tgtgcgagga atgtcacacg ccgcgggacg gcggcggcaa tctggacgag tcccggcggt
      421 tgcaaggcgc gccgatctgg atcacgcccg ttcaccacaa tacgaactgg gccatgaatg
      481 cgcccagttt agcgggattc gaacagttta ccgaagaaca gggtgagcaa attctcgagc
      541 acggagtcgg tccgaacgga ctgcccatcc agccgccgat gcacatctac cacatgaatc
      601 atgcggatgc gcaagcgatt atcgcttacc tgcgctcgct gccgggcggc tatccgcagc
      661 ggtaacccca cgtcgctcaa ccacaaccga acgccagtat aatcgcgggc gatggaagag
      721 catctaaagg cgtgggtgga atggctgctc ggcccggaga cttccgacga aaaacacctg
      781 ggacatctct ggccgcgatg gattttcctg cgggctctcg ggctcatcta tttttccgca
      841 ttctattcgc tgatctttca aatcaagggt ctgctgggtc ccaagggaat tcttccggcg
      901 gcagattacc tgcaggcggt aaccgcggcg cttcactcgg cgcggttctg gttcgcaccc
      961 acactttttt ggcttagttc gagcgaccgc gcgctgatgt tggtttgctg ggttgggctt
     1021 gcggcgtcca ttctgctggt gctgaatgtt tggccgcggg cagcgctggc ggtctgtttc
     1081 gtctgcttcc tctcctttat tggcgcagcg caggactttg cgagctacca atcagacggc
     1141 atgcttctag gagcgggctt cgtcagcctg tttttcgcgc cgccgggatt tttgccgggg
     1201 ctgggccgcg cgaatccgcc atcgcgcgca agcctttttc tgctgctatg ggagtggttc
     1261 cggatttatt tcgaatcggg cttcgccaaa atggccagcg gcgatacctc atggcgcaat
     1321 ttcacggcca tggatgacta ctatcagaat gggccgctgc cgacgtggat tgggtggcat
     1381 gtgggtcacc tgccacactg gtttcacgcg tcgtccgtct tctatacgct ggcagtcgaa
     1441 ctggtgctgg tgtggatggt gtttttgccg cgccgatttc ggatcgcgtg tttctggatt
     1501 gttgcgccgt ttgaaatcag catcattctc acgggcaatt acgcattctt gaattatctg
     1561 gttttttcgc tcggcttcct cttgctcgac gatcgtttcg tggaatggat cgttccacag
     1621 cggattcgag gcgcgttcga acgcaagcgc gatggagtac cagcgccgga agcgctgcca
     1681 gatgattggc gcagcgcatg gcgcaaacgg ctcaaacccg tgcgtatgac agtggcagga
     1741 atttgcttgg gtcttgtttt ttacgcgacg accgcgattt tcatctggac gctggtgccg
     1801 aacgttccgc tgccacaaaa gcccgtgacc atgctcgaac catttcgcat cgcaaaccgc
     1861 tacgccctct tcgccaacat gactcatcag cgatacgaga tcgagttcca aggctcgaag
     1921 gacggaaaga cctgggttcc atatccattc cgctacaagc cgcaggatcc gagcaaaccg
     1981 ccgggaatct atgcgcccta tcagcctcgc tttgaatgga atctatggtt tgcgtcgctg
     2041 ggaaattggc gcgagtatcg cttcgttttg tggacggagg aacgactgct gacaaacgag
     2101 ccggacgtac tggagttgtt cgcgcgcaat ccttttccgg gtacgccgcc gaaggaggtg
     2161 cgcagcgtgc gataccaata ttggttcacg agcgaggaaa ccaggcggaa gcaaggactc
     2221 tggtggcggc gagaaatgct gggcgaatac gcgccgacgc tcgagcgcga gcccgatggc
     2281 aagatcgtaa tactggattt tccaggcgac gaagcgccca caaggtagca aaacttcccg
     2341 gtaaatctac ttggcgttat tcgatgattt gttcacggcg tcgagttgag tctgcaaatc
     2401 agcgactttc ttttccgcat cggtgacttc ctgctgcttc gaggcaacct gatttttttc
     2461 gtcctccagg gcggcagcga tggctgtatc ggacgaatat tcaggcttgc cgtaataagt
     2521 ctgctggtcg agcccatatt tccgctgaag taaatcgagg tcgaccttaa gcttgtcgag
     2581 attggctttg gccgcgctga ggtctgcctc gagcgccgac ttatcggcgg cgggcggatt
     2641 actggccgcg gatttgtcgg cagccgccgg cgccggattg cttggagggg cggggggctg
     2701 gcctacaatg cttatgtcgc cgtgcaagga agccaggctc tcgttgtccc aaaccttcgg
     2761 gggtttcgcc tggtccttct tagcttcgcg cgcgcggcga gcggcggccg cgagtgaatt
     2821 ttgctgctgg cccgatgcgg acggtgtttg agcctgcgat tgccgtgccg ctgtagcgcc
     2881 tgacagcgaa agcagcagga tgaggggtgc ccatttcgcg ataaacattc gattcttcct
     2941 ccaacacgac tctttcgctg ttctatggta tcgcaggcgc agacgcccgg gaagttgcct
     3001 cggcggcgga gtctaaagag gtaactgcaa gagcagcggg cccctaaact gtacgcctga
     3061 ccagttgcgc cttgccgtac agagatggaa aatacacagg taatttagtt gcgcaggcgc
     3121 accgcagttt cttagactga gcatcggctc acccgcgccg gggcgttgcc gcatctaatt
     3181 tgtgccacat gaaaacgatc ccagctctta gacaagccgt ctcgattttg tgcctcaccc
     3241 taattagcgt tcccgcaact gcttttggga aggcccccgc ccgtcatcgt cattacaggc
     3301 accgcgcgag ggtcgttcag tacgtcaacc ccgcgaagga cgacatcgta atcaacgatg
     3361 atccggtggt ccgcaatatt gccgtggatg ccttggggca cgaaaaagga tccgtgttgg
     3421 cagtggatcc aacgaacggc cgcattcttt ccatcgtgaa tcagaaactg gccttttccg
     3481 ccggcttcga gccgtgctcg acaatcaagc cattcgtggc gctggcagcg ctgaaggaag
     3541 gcgtcatcac gcgcgacacc atgatacggg tggaatcgcg ccgctatatc aatctgacag
     3601 aggcgatggc gcattccaat aacaagtttt tcgaggaagt cggaaggcgg ctcggttttg
     3661 atcgcgagtt gaaatatgac gcgctgctgg ggctggggca gcgtgtcggt tacggcattc
     3721 ccgaagagca gcctggatcg ctgccgccgt cggcgcctct ttttggaggg gtggcgcgca
     3781 tgtcgagctt cggcgaaggc atccgcatca cgccttttca gcttgcatcg ctggttggaa
     3841 tgctggccaa tggcggcacg caatattatc tgcagtatcc tcgcaccgaa gctgcgcgcc
     3901 agaacttcga gcctcggatt cgcgagcggt tcgatatcgc tccgttgctt ccggaccttc
     3961 gagagggaat gctcgccgcc gttctctacg ggacggccaa gcgtagctac gacccggatg
     4021 gcgaacagga cctcgggaag acggggacgt gtaacgatga aggcgtaggc ggacgcctcg
     4081 gctggtttgt ttcctacgcc gaccaggctc acccaaaaat cgtgattgtg gttttgctcc
     4141 acggaggcgc ccgcattatc agcggacccc acgcctcgga gattgccggc aggatctacc
     4201 gcggactcta ccagcgaaat tacttcgccc gggaagattc tgcgtcgagg caatacgcgt
     4261 catctccggc gccgtccggc tctgttcctt ccccctaaaa tcaggccatg aggccgtcgt
     4321 ccgtgcttcg aaaaacagct tacgatgccg tggttgtcgg ctcggggcca aacggtcttg
     4381 ctgctgcaat cgtgatggcg aaagccgacc gatccgtcct ggttttggag gctgaagatt
     4441 cgattggggg cggaacgcgt tcggcccagc taacccttcc cggatacgtc catgatatct
     4501 gttcggccgt gcatccgatg gcattggctt caccgttctt tcgatcgctt cctttggagg
     4561 aacatggttt ggaatggatt tatcccccgc ttccgctagc tcacccgcgt gacgacggca
     4621 ccgccatggt gcttgatcgg tccctcgacg ggacatgcga atcgcttggt gtggacgcgc
     4681 agagctaccg caatttgctt ggtccgctgg tgcgaaattg gggtgtcgtt gaaagcgcga
     4741 tcctcgggcc tctgggtgtt ccgcgaaatc caatcgcaat gtcccgattt ggcctgcatg
     4801 ccatccagcc tgcggcccgc cttgcgcgca ataagtttca gacggaggcg gcgcgagtgt
     4861 tgttcgcagg tttagcctgc cattcggttg ttccgctaga gaagtgggga acggcggcga
     4921 ttggccttgt gttggcggcg gtggcgcacg tttacagttg gcccattccc aagggcggct
     4981 cccagcgcat cggagatgcg cttgcttcgt atctgcgttc tctgggtgga gaaattcaat
     5041 gcggcgttcg ggtggagtca atcgacgaca tcccttccgc acgcacgatc ttctgcgact
     5101 taacgccgca aggacttctc aaaattgctg cgcaagttct tcccgccaat tatcagaaag
     5161 cattgagaag atataaatat ggtcccggcg ttttcaaagt cgattgggcg ctgtcgaatc
     5221 ccattccatg gaaggctaag gaatgttcgg gagccggcac ggttcacgtc ggctcgtcac
     5281 ttgaagaaat cgaacgctcc gagcgcgccg cgtggaacgg gtcacaagat gacaaaccgt
     5341 ttgtgctggt gacgcaaccg agtctgttcg actcgacgcg tgctccggta ggtcgccaca
     5401 ccgcctgggc ttattgccat gtatcaaata acagcgattg cgatatgacc agccgaatcg
     5461 aatcgcaaat cgagagagtc gcgccgggtt ttcgagatgc cattcttgcg cgcagcgtca
     5521 gggggccggc agagctgcaa cggcacaatg caaatctggt gggcggtgat atttccggcg
     5581 gagcaaacaa cctgatgcag ctcttttttc gtccgacgcg ccgcacctat gcaacgccgc
     5641 atcgtggatt gtatctttgc tcgtcatcca ctccgccagg gggcggtgtg cacggaatgt
     5701 gtggttatca cgccgcggta cgcgcgatag ctgccggaga atagcgaacg gcgccgaaac
     5761 tggacgatca tccgctccga atagcttcaa ccagaactct tgcgcgcagg cgcgccgcgg
     5821 tgtatacgtc acacgcaagg atttccgagc acgatagttt acggaggcgt cggcatgagg
     5881 cgttggctca cactcttttc ctttttcatt tgtctggcga tgtttgtagt tgtaccggaa
     5941 caatcc
//
LOCUS       DATNDT010000480         5428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3865939, whole genome shotgun sequence.
ACCESSION   DATNDT010000480 DATNDT010000000
VERSION     DATNDT010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5428
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3865939"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /gene="dnaJ"
                     /locus_tag="VN788_15800"
     CDS             <1..966
                     /gene="dnaJ"
                     /locus_tag="VN788_15800"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HXU22047.1"
                     /translation="SDPQKRAIYDRYGHAGLNSRGFETSGGFNSTIFEEFQDILGDFF
                     GFEGAFGGGGGRRGRAQRGQRGADLRYDLTLSFEEAAAGVTSTIKLDRFENCELCHGT
                     GAKPGSGMVACRTCGGRGQMAYQQGFFSISRTCPACQGSGQVVREACEKCRGQGRLQR
                     EKTLEVGVPAGVDSGTRLRMAGQGEPGVNGGPPGDLYIFLEVKEHPLFERRGADLYCT
                     VPISFPQAALGATVKIPTLHGEEDLEIPEGTQSGQLFRKRAKGLPDPHGGRGDLYVNV
                     RVVVPSKLTREQKRALEQLGQTLKVDNKPAERSSSFFDKVKDIFG"
     gene            complement(1029..1790)
                     /locus_tag="VN788_15805"
     CDS             complement(1029..1790)
                     /locus_tag="VN788_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016547281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HXU22048.1"
                     /translation="MPSVVNPDRPWPVPSSPWIMSQKWHDLLFMHWRVDPNLVRRLIP
                     ASLELDTYEGSAWVGVVPFRMSGVSPRWTPPIPGVSALPELNVRTYVTRDGKPGVWFF
                     SLDAGSALAVAAARRFFYLPYFRAQFSINTASDGTIDYRSRRTHRGAAAAELRMSYKP
                     AGEVFHAQPGTIEYFLTERYCLYAFDGTRIFRCEIDHAPWPLQPAEAQIKVNTMAAAS
                     AILLPNEKALLHFARYQYVKIWGLKEVNRESQRSK"
     gene            1909..2910
                     /locus_tag="VN788_15810"
     CDS             1909..2910
                     /locus_tag="VN788_15810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HXU22049.1"
                     /translation="MIRRACLLLVVLALGARISRAQQNEPLRLAQTIPLPNVHGRIDH
                     FDVDLQGHRLFMSALGNNTLEVFDLRSNKVIYAIRGLREPQGVTYVPKSDRIFVANGD
                     DGTIRMFDGATYKPLKMAHVSSDADDSRYDATTNHVLVGYGDGTDAGIAILDGATGSL
                     VATIKLPGHPEGFQLEKNGERVFVNIPSVNNIIEVVDRNTRKIVTTWQLGDARGNFPM
                     ALDETDHRLFIACRTPPQTLILDTESGKIIARVPSVTNADDAWYDAANKRIYVSGGGG
                     FVTVIGRQDANHYREVSQIKTADGARTSCLAPELNRLFVGVWGRGGEPEELQIYDIQ"
     gene            complement(2999..3562)
                     /locus_tag="VN788_15815"
     CDS             complement(2999..3562)
                     /locus_tag="VN788_15815"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HXU22050.1"
                     /translation="MNQYGFRLYSFPCKYDCDASFKHVPDTPYLNSLCGFPQTTQREK
                     DPIPRDGLFRRFRGTALKFDGRARMKQDHDMETLAKRLREAREFLGMSQEYVAQQMNV
                     PRPAISKIESGRRKVDSLELIRLSKLYGRPVGYLLGESQEEETFNTDPLAAKLRLMTR
                     GLRAEDREEILHFIEFLRHRKTAYQTH"
     gene            3837..4382
                     /locus_tag="VN788_15820"
     CDS             3837..4382
                     /locus_tag="VN788_15820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22051.1"
                     /translation="MRLTSKLAVTAGLLALAMVPRSNAQALNSNTANVNLNAVLAETL
                     TVSATPGTVNFNLVPSGPVSGTTPVSITTQWALASTRTKLAVWAYFSTTTALTNGAGN
                     NIPNTSVSGNPDSTGLQPFSATNPFNGLATGVQIYNTAITAANVNSTHSDSLNLQIDT
                     TGLNLPAGTYTGVLNIEAQAL"
     gene            4584..5138
                     /locus_tag="VN788_15825"
     CDS             4584..5138
                     /locus_tag="VN788_15825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22052.1"
                     /translation="MSRLRPVIYVVLFALTTLALVGPGDGAQVSSNIAGVNLSAILGD
                     SISVSASPGLVNFALVSNGVATGSTTITVNSGWVLGHPATISSYAYFSNSTAALTDGA
                     GHNIPSSSVSGKVDGGGFQTFTGACPFSANTCVQIWTKHVPSAGIKLTGSRSDTLQLQ
                     ISTVGLSLQAGTYTGVLHIQAQAL"
     gene            5222..>5428
                     /locus_tag="VN788_15830"
     CDS             5222..>5428
                     /locus_tag="VN788_15830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22053.1"
                     /translation="MGRTISFLLIFLVFGAWHTQAQTISPVIVEYQQKADGRFQVTND
                     SDTPLAVVLEPESFRVDSKGNPTFY"
BASE COUNT         1196 a         1431 c         1614 g         1187 t
ORIGIN      
        1 tccgatccgc agaagcgcgc gatttacgat cgctatggac atgcgggact aaatagccgc
       61 ggctttgaga cgagcggcgg gttcaattcg acgatctttg aagagtttca agacattctg
      121 ggcgactttt ttggatttga aggcgcgttt ggcggaggtg gcggacggcg cgggcgtgcg
      181 cagcgcggtc aacgtggcgc ggatttgcgt tatgacctga cgctgagctt tgaagaggcc
      241 gcggcgggcg taacgtcgac gatcaagctg gaccggtttg aaaattgcga gctttgccac
      301 ggaactggcg cgaagccagg tagcggaatg gtcgcgtgcc gcacgtgcgg cgggcgcggg
      361 cagatggcgt accagcaggg tttcttttcg atttcgcgga cgtgcccggc gtgccaggga
      421 agtgggcagg tggtgcgcga ggcttgcgag aagtgccgcg ggcagggacg attgcagcgc
      481 gagaagacgc tggaggttgg cgtaccggcg ggcgtggatt cgggaacgcg gttgcgcatg
      541 gccgggcagg gcgaaccggg tgtgaatggc gggccgccgg gcgaccttta tattttcctt
      601 gaggtgaagg agcatccgct gtttgagcgg cgcggggcgg atttgtattg cacggtgccg
      661 atcagttttc cgcaggcggc gcttggcgca acggtgaaaa tcccgacgct gcatggcgaa
      721 gaggatctgg aaattcccga aggcacgcaa tcgggccagc ttttccggaa aagagccaag
      781 ggcttgccgg acccgcatgg cgggcgcgga gatctttatg tgaatgtgcg ggtcgttgtg
      841 ccgagcaaat tgacgcgcga acagaagcgc gcgttggagc agcttgggca gacgcttaag
      901 gtggacaata aaccggcgga gcggtcgagc agcttttttg acaaagtgaa ggatattttt
      961 ggatagattg atgaggtaga gaaagtaaag gagaggaacc tattttcgtc ctagcacaga
     1021 tgcgaccgtc atttacttct ttgggattcc cgattcactt cttttagtcc ccagattttt
     1081 acgtactgat aacgcgcgaa gtgcaagagc gctttttcgt ttggcaacag gatggcgctg
     1141 gctgctgcca ttgtgttcac cttgatttgc gcttcggcgg gctgcaacgg ccagggggcg
     1201 tggtcgattt cgcagcggaa aatgcgggta ccgtcaaagg cgtagaggca ataacgctcg
     1261 gtgaggaagt attcgattgt gccaggttgc gcgtggaaga cttcgccggc cggcttgtag
     1321 gacatgcgaa gctctgcggc tgcagcgccg cggtgcgtgc gccggctgcg atagtcgatc
     1381 gtgccatccg aggctgtgtt tatcgaaaat tgcgcgcgga agtatggcag atagaagaag
     1441 cggcgggcgg ccgccactgc caacgcgctt cctgcatcca aactgaaaaa ccacacgccg
     1501 ggtttgccat ccctagtgac gtaggtgcgc acgttcagtt cgggcaaagc cgagacgccc
     1561 ggaataggag gcgtccatcg ggggctaact ccggacatgc ggaagggaac tactccgacc
     1621 caggcgctgc cctcgtacgt gtcgagctca aggcttgcag ggatgaggcg gcggactaga
     1681 ttcggatcga cccgccaatg catgaagagc agatcgtgcc atttttgaga catgatccaa
     1741 ggcgaggatg ggaccggcca agggcgatct ggattgacga cggacggcat gaatacgaca
     1801 gtatagcgga gtcgtagtga tcagaagtat gttcgggcat agtagaagag gagggaaaat
     1861 gcgccatcac gaacgtgagc gcgtttttac gccgctagag agtacccgat gatccgaaga
     1921 gcttgcctcc tactggtggt attggcgctt ggcgctcgga tctcgcgcgc ccagcaaaat
     1981 gagcctctcc gcttggcaca gacaattccg ttgccgaacg tgcatgggcg catcgatcac
     2041 ttcgacgtgg acctgcaagg gcaccgtctg tttatgagcg ctttgggtaa caacactctt
     2101 gaagtgttcg atctccgatc gaataaagtg atttacgcga ttcgcggatt gcgcgaacca
     2161 caaggagtaa cttacgttcc gaagtcagat cgcatcttcg tggcgaacgg cgacgatgga
     2221 acgattcgga tgttcgatgg cgccacgtac aagcctctca agatggccca tgtctcaagc
     2281 gacgccgacg atagccgcta cgacgcaacg acaaatcacg tccttgtggg ctatggagat
     2341 ggaacggatg caggcatagc cattctggac ggcgctacag gcagtttggt cgcgacgatc
     2401 aaactgcccg gtcatcccga aggatttcaa ttggaaaaga acggagagag ggtctttgtg
     2461 aacatcccct ccgttaacaa tattattgaa gtggtggacc gcaatacgcg aaaaattgtc
     2521 acaacgtggc aactgggcga tgcgcgcggc aatttcccga tggcgctgga tgaaacggac
     2581 catcgcctgt tcatcgcctg ccgcacaccg ccgcagacgc tgattctcga caccgaatca
     2641 gggaagataa tcgcccgagt cccgagcgtt accaatgctg atgatgcgtg gtacgacgca
     2701 gcgaacaaac gcatttacgt gtcgggtggc ggcggattcg tcacggtaat tgggcggcag
     2761 gatgcaaacc attaccgcga ggtttcacag ataaaaactg cagacggcgc gcggacttct
     2821 tgcttggcgc cagaattaaa tcgacttttt gtgggcgttt ggggccgagg tggcgagccc
     2881 gaggagctgc agatttacga catccaatag cgcgaaattg aggattcggg ggatagatgg
     2941 ttgcgtggcg catacatcgt ttccccagcc ggtacaaccg gaattcgccg ccatgcggtt
     3001 agtgcgtctg ataggcagtc ttccggtgcc tcaaaaattc gataaaatgc aaaatttcct
     3061 ctctatcttc tgctcttagg ccgcgcgtca ttaatcgcag tttggctgcc agtggatccg
     3121 tattgaatgt ctcttcttcc tggctctcgc caaggagata tccaactggg cgaccgtata
     3181 acttcgagag cctgataagt tccagactgt cgaccttgcg ccggccgctt tcgatttttg
     3241 atattgccgg tcgcgggacg ttcatttgtt gcgccacata ttcctgcgac attcccaaga
     3301 attcccgagc ctcgcgcagg cgctttgcca gtgtttccat gtcgtgatcc tgtttcatcc
     3361 ttgcccgccc atcaaatttc agggctgttc ctctgaagcg ccggaacagc ccgtcccttg
     3421 ggatagggtc tttctctcgc tgagtagttt gggggaagcc acacagtgaa ttcaaatacg
     3481 gagtgtccgg aacatgttta aacgatgcat cgcaatcata cttacaggga aagctgtaaa
     3541 gtcgaaagcc gtactgattc atgtacggga taacagactc ggggagtcag cagccgattg
     3601 gcagctttga gcgcaaccca ctcataagac agctttaaca tccgctctaa atgcctgtaa
     3661 gttgttccaa aaagataata ttcgccgttc caaataagta cagaagtcct attctgaaac
     3721 gcgatcctcc ctgttgaact tgtatgcaga acgcaaggga aataggttct aggcaagacg
     3781 ttctagcccg ttctaatcag tccgagtctt tactcggcta aatctgcgga ggatagatgc
     3841 gactcaccag caaattagcg gttaccgcgg gactcttggc tttggctatg gttccccggt
     3901 ctaatgcgca ggcgcttaac tcgaatactg ccaatgtaaa cctaaacgca gttctggcgg
     3961 aaaccctgac cgtatcggcg acgcctggaa ccgtgaactt caacctggtc cccagcggcc
     4021 cggtgtcggg cacaacgccc gtcagcataa cgacgcaatg ggcattggcc agcacacgta
     4081 ccaagcttgc cgtctgggcg tatttctcca ccactaccgc attgacgaac ggtgccggca
     4141 ataacatccc gaacaccagc gtttcgggaa acccggacag cacgggcctc cagccgttct
     4201 cggctaccaa ccccttcaac ggcctcgcca ccggcgtgca gatttataac accgcgatta
     4261 cggcggcgaa cgtgaactca acgcactccg actcgctgaa cctgcaaatc gatacgaccg
     4321 gcctgaatct gccagcgggc acctacacag gcgtcctaaa catcgaggcg caggcgctgt
     4381 agtttccgta gacactgagg attcggacga agtcctagtt tgacggcccc cggatcgaag
     4441 ggaggcgacc tgagtcggcc gcctcaccta ttctcagcct ctccgaaaca gcttcagaca
     4501 ccaggagacc agacctgatt cgcatagaca aatccgagcc acagtctctt ctgccaccga
     4561 aattgcccgc agttgcgcgg caattgtctc ggttgcggcc ggtcatatac gtggtcctct
     4621 tcgctctcac cacccttgcg cttgtcggac ccggagacgg tgcgcaggtc agttccaata
     4681 ttgccggcgt gaatttgtca gcgatattgg gggattccat ttccgtttcc gcgtcgcccg
     4741 gcttggtgaa ttttgcgctg gtttcgaacg gcgtcgcaac gggcagcacc acgattacgg
     4801 tcaatagcgg ctgggtactc ggccaccctg cgacgatcag ttcctatgca tatttttcga
     4861 attcgacggc agctttgaca gatggcgccg gccacaacat tccttcgtca agtgtttcgg
     4921 ggaaggttga tggcggcggc tttcaaacgt tcactggcgc ctgtcctttt tcagcgaaca
     4981 cctgcgtgca gatttggact aagcacgtgc catcagcagg gatcaagctc accggctcgc
     5041 gcagcgatac gttgcagctt caaatcagca cagtagggct tagtctacaa gcaggaacct
     5101 atacaggcgt acttcacatt caggcgcagg cgctctgacg atgttggggg cggagcaaac
     5161 cgcgtggcgc gcaagcgtcc gtagcaaagg ttgtttgatt actctcttgg ggagaagctt
     5221 catggggcgc acaatctcgt ttcttctgat ttttctcgtc tttggtgcct ggcatacgca
     5281 ggcgcagacg atttcaccgg tcattgtcga gtaccaacaa aaggccgacg ggcgcttcca
     5341 ggtcactaac gattcggata caccgctcgc tgtggtgctg gagcctgaga gcttccgtgt
     5401 agattcgaag gggaacccaa ctttctac
//
LOCUS       DATNDT010000481         4207 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4156641, whole genome shotgun sequence.
ACCESSION   DATNDT010000481 DATNDT010000000
VERSION     DATNDT010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4156641"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2889
                     /locus_tag="VN788_15835"
     CDS             <1..2889
                     /locus_tag="VN788_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR03437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22054.1"
                     /translation="DTANNRVLAWKNSTGFTNGATADLVVGQRDKLSTSQQGPGRGLS
                     TGLAAPVAVAVDAIGNLYVVDAGNNRVVRYPKPFTQSGGFLTVDLIIGQPDLNSSQSN
                     QGQAAPSAQTLALISGSTALRSGVVFDSQGDLWISDAGNNRVLRFPASALANGAANDP
                     AADLVLGQTDFTSNSVPSGTTRQTKNALAQPSALGFDAVGRLFIADDGARVLVFVPGF
                     FSGMSAARIMGVVVPNAQQPNPPAVGASTLGAQGKPATGIVFVGNTPFVVDGANNRIL
                     KYDSYDKWLPEAAAFSPAATAVIGQRDFSSSKANQGQPDASGSTLNSPAGAAFFGNDL
                     YVADSGNNRVLDFPQQGTAIFSAAAHVLGQIDFPYFAPNLIEGREFFFFAGNVNENGI
                     TVAYPGGAVAVDTNSNPPRLYVSDPGNNRILGFKDARNVHPGQKADIVIGQPDFYRDQ
                     INYPKNDSSQPSDTGLFLPEGIAIDKNGDLWVADSGNGRVVRYPSPFQQKNSPLQANL
                     VLGQFGFTTKVTDPSPETMSSPYGVALTQEGDLAVSDSIHNRVLLFLRPASSDFTNGQ
                     PAAEVIGQPDFVTTTAGTPANNGFASPHLIAIDAFDRLYVADTGNNRVAIYRYIRGIG
                     NNPSASFSIASFTGGYLNAPHGVYVDPSSGQIWVANTFGNTVLRFPNYDQLLINPTPT
                     VALQVLEPVAVTVDAFGNPVIAEGANRVSFYYPQLDARNGANYFHRYAPGMLASLFAL
                     PGVQFSNSTQNFDSLPHPLPIPSTLADTQVLVNGAAAPVLYVSPAQINFQVPSATPTG
                     TQEFQAVRASTGQVLASGFFPVEQTSPALFTADSTGAGQVSAIDADNGKPNSGTNPVK
                     AGHYISLYGTGQGDVPGGPLDGNVAQGPVNTPQTPQVYIGGPNFVPSGDIQYSGLAPG
                     LVGLWQINVKVPATAAPGPVQVLVQLNGSASSVDALQERVVTTIYVTQ"
     gene            2923..3795
                     /locus_tag="VN788_15840"
     CDS             2923..3795
                     /locus_tag="VN788_15840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HXU22055.1"
                     /translation="MAFCTKCGNALAVDARFCTNCGQSLNGPVGTASAVAAVPKIDYT
                     LEGSNLQIARINLKPGQEIYAEAGRMVYKTASVSWETRMTGESLGEKLWGAFKRRLMG
                     QSIFLTYFRPSGDGEVGFAGSYPGKIQAYELAPGQTVIVERDSFLAAQSSVRLDIALV
                     KKLGAGFFGGEGFILEKLTGPGLVFIHAGGDFVEFDLKPGEIIQVETGNIVGFDESVS
                     YDIQLAGGIKTAMFGGEGLFLATLTGPGRVLIQSMTLQRLRRELVPYRHDGDEHKGLD
                     ALSGMFDNSFFDAN"
     gene            complement(3797..>4207)
                     /locus_tag="VN788_15845"
     CDS             complement(3797..>4207)
                     /locus_tag="VN788_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III-like domain-contianing
                     protein"
                     /protein_id="HXU22056.1"
                     /translation="VDQLPPFDNYSMDGRTYRYFRGEPLYPFGHGLSYSTFHYSDLQA
                     RRDGGKLQVSVRVTNTSQRDGDEVVQLYVSRQSEPDAAVRDLRGFQRIHLSAGESRNV
                     QFAIPAASGQSRISVGGGQPLPKWASGRYVETTE"
BASE COUNT          864 a         1260 c         1164 g          919 t
ORIGIN      
        1 gatacagcca acaaccgcgt cctggcgtgg aaaaattcga ccggattcac gaacggcgcg
       61 accgccgatc tggtcgttgg gcagcgcgat aagttgtcca cgtcgcagca aggcccgggg
      121 cgcggtctca gtactggact ggccgcgccg gtcgcggtcg ccgtcgatgc aatcggaaat
      181 ctctatgtcg tcgatgcggg caataatcgc gtcgttcgtt atccgaagcc gttcacgcag
      241 agcggcggct ttctgacggt cgatctcatt attggacagc cggatctcaa tagcagccaa
      301 tcgaatcagg gccaggccgc tccgtccgca caaacgctcg ccctcatctc gggaagtacg
      361 gccctgcggt ccggcgtggt gttcgattcg caaggcgatc tgtggatttc ggacgccggc
      421 aataatcgtg tgctgcgttt cccggcgagc gctctcgcaa acggcgcggc caacgacccg
      481 gccgctgact tggttctcgg ccagacggac ttcacaagca actcggttcc atccggcacc
      541 actcgtcaga cgaagaacgc tcttgcgcag ccttccgctc tcggtttcga cgcggttggc
      601 cgcctattca tcgcggatga cggcgcacgc gtgctggtat ttgtcccagg tttttttagc
      661 ggcatgtccg ctgcgcgaat catgggcgtt gttgtgccaa atgcgcagca acctaaccct
      721 ccggctgtcg gcgcgagtac gctcggcgct cagggaaagc ctgcaaccgg aatcgtcttt
      781 gtaggcaata cgccgtttgt tgtcgatggc gcgaataacc gcatcctcaa atatgattct
      841 tacgacaagt ggcttcccga agccgccgca ttttctccgg cggcgacggc agtaatcgga
      901 cagcgcgatt tcagttcaag caaagccaat caaggccaac ccgatgcgtc tggcagcacc
      961 ctaaactcgc cggccggagc ggccttcttc ggcaacgatc tctacgtagc tgattcggga
     1021 aataatcgtg tgctcgattt tccgcagcaa ggcacggcca tcttcagcgc cgccgctcac
     1081 gtgctcggcc agatcgattt cccttatttc gcgccgaatc tgattgaagg ccgcgagttt
     1141 ttcttcttcg ccggaaacgt caatgaaaac ggcatcacgg tggcttaccc cggcggcgcc
     1201 gtcgccgtgg acacgaactc caatccgccg cgcctgtacg tttcggatcc cggcaacaac
     1261 cgcatcctag gcttcaagga cgctcgcaat gtccatcctg gacaaaaggc ggacatcgtc
     1321 atcggacagc ccgattttta tcgcgatcaa atcaattatc cgaagaatga ttcaagccaa
     1381 ccaagcgaca cgggtctgtt tctgccggag ggaatcgcga tcgataaaaa tggcgatctt
     1441 tgggtcgccg actcgggtaa cggtcgcgtc gtgcgatacc cctcgccctt ccagcagaaa
     1501 aattctccat tgcaggcaaa tctcgttttg ggacagttcg gcttcacaac gaaggtaacc
     1561 gatccgagcc ccgaaacgat gagttctccc tatggcgtag cgctgacgca ggaaggcgat
     1621 ctggccgtgt cggattccat tcacaatcgc gtcttactgt ttctgagacc ggccagctcc
     1681 gatttcacca acggccagcc cgccgctgag gttatcggcc aacctgactt cgtgacgacc
     1741 acggccggta cgcccgcgaa caacggtttc gccagtccgc atttgatcgc gattgacgcc
     1801 ttcgaccgcc tttatgtcgc ggatacaggt aacaatcgcg tcgcgattta ccgctacatc
     1861 cgcgggattg gaaacaatcc ctcggcgagt ttttccatcg cttcatttac tggcggctat
     1921 ttgaatgccc cgcatggcgt gtatgtcgat ccttcatccg gacagatctg ggtggcgaac
     1981 acgtttggaa acaccgtgct ccgattcccg aattacgatc agttactcat caaccccact
     2041 cccaccgtcg cgcttcaggt ccttgaaccg gtcgcggtaa ctgtggatgc cttcggaaat
     2101 ccggttatcg ccgaaggtgc aaatcgcgtt tcgttttatt acccgcaact cgatgcccgc
     2161 aatggcgcga actacttcca tcgctacgcg cccggcatgt tggctagtct gttcgcgctg
     2221 ccgggggttc aatttagtaa ctccactcaa aacttcgact cgcttcccca tcctttgcca
     2281 atcccgagca cgctggcaga cactcaggta ttggtaaacg gagccgcggc gccggttctt
     2341 tatgtctcgc cagcgcaaat taattttcag gtgccatccg cgactccaac gggtacacag
     2401 gaatttcagg ctgtgcgcgc atcgacgggg caagttctcg caagcgggtt ttttccagtt
     2461 gaacaaacgt cgcctgcctt attcactgcc gacagtaccg gcgcgggcca agtgtccgcc
     2521 atcgacgcgg ataatggcaa gcccaatagc ggcacaaatc cggtgaaagc cggtcactat
     2581 atatcgctat acggcaccgg ccagggcgat gttccaggcg gccctctgga tggcaacgtg
     2641 gcgcaaggac cggtaaacac gccccagact ccgcaggttt atattggtgg cccgaacttt
     2701 gtgccttccg gtgatattca gtattccgga cttgcgccgg gcttggtcgg tctgtggcag
     2761 atcaatgtga aagtgcccgc gactgccgcg ccgggtccgg tgcaggtatt ggtccaactg
     2821 aatggcagcg ccagcagcgt cgacgcgctg caggaacgcg tcgtgacgac gatctacgtg
     2881 acgcagtaac tcatgtaaga taaaacctcg gagacctacg ttatggcttt ttgcactaag
     2941 tgcggtaatg ctttagccgt cgatgcgcgc ttctgcacca attgcgggca gtcgctcaac
     3001 ggtccagtcg gaacagcatc ggcggtagct gccgtgccga agatcgacta cacgctggag
     3061 ggcagcaact tacaaattgc ccgcatcaac ctgaagcccg gtcaggagat ctatgcggaa
     3121 gccggacgca tggtttacaa gacggccagc gtttcctggg agacgcgcat gaccggtgaa
     3181 agcctgggtg agaaactctg gggagctttc aaacggcggc tgatgggaca atcgattttc
     3241 ctcacttact ttcgtcccag cggggatgga gaggtcggct tcgccggcag ttatccgggc
     3301 aagattcagg cttacgagct tgctcccggc cagaccgtca tcgttgagcg tgacagcttt
     3361 ctcgccgcgc aatcgtccgt tcgtctcgac atcgccctcg tgaagaagtt gggagcgggt
     3421 ttcttcggtg gcgaaggctt tattctggaa aaactgaccg ggccgggcct cgtattcatc
     3481 catgcgggcg gcgatttcgt ggaattcgat ctgaagcccg gtgagatcat tcaggtggag
     3541 acgggtaata tcgtcggctt tgacgaatcg gtcagctacg atattcaact tgccggtggc
     3601 atcaagacag ccatgttcgg cggtgaaggc ctttttctcg cgacgctcac tggtcccggc
     3661 cgcgtcttga tccagagcat gacgctccaa cgtctacgcc gtgagttagt cccctaccgc
     3721 cacgatggtg acgagcacaa aggactcgat gccctgagcg gcatgttcga taacagcttt
     3781 ttcgacgcca actaacttac tcggttgtct cgacgtaacg tccgctggcc catttcggca
     3841 gcggctgccc tccgccaacg ctgatccgtg attggccgct cgccgctgga atggcgaact
     3901 gcacattacg gctttctccg gcggaaagat gaatccgctg aaacccgcgc agatcgcgga
     3961 cggccgcatc cggctcgctt tgccggctta catagagttg aactacttca tccccatcgc
     4021 gttgcgacgt gttcgtcacg cgaacggaaa cctgaagctt gccgccatct cgccgcgcct
     4081 gaagatccga atagtgaaat gtcgagtaac ttagcccgtg accaaacggg tacagcggct
     4141 cgccacgaaa gtatctataa gtccttccgt ccatcgaata attatcaaac ggcggcaact
     4201 gatcgac
//
LOCUS       DATNDT010000482         3750 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_125606, whole genome shotgun sequence.
ACCESSION   DATNDT010000482 DATNDT010000000
VERSION     DATNDT010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3750)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3750)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3750
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125606"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="VN788_15850"
     CDS             complement(<1..861)
                     /locus_tag="VN788_15850"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HXU22057.1"
                     /translation="MKKPMRYTHRAMEAPNVDFNLSRLERVISGPGKVAALGEELDRR
                     GLKRAIVVTGKSLGASPLLTKVTAPAGARCAAVFKGARQHVPRSVVDHLQREIERVEA
                     DCLISFGGGSPIDACKVASHAFLPKREIIQIAIPTTLSAGEYTHAGGVTDEKTLVKSG
                     VSDPRLQPRTVINDPELTLETPEWLWIATGMRALDHAIETIYAIRHHPLSDALGAKAI
                     ALLVEHLPRSIKTSGAERLAHRGYCQFAAWFSIFGSMNTRFGISHLLGHQIGPRWNVA
                     HGITSCITLPH"
     gene            1020..2174
                     /locus_tag="VN788_15855"
     CDS             1020..2174
                     /locus_tag="VN788_15855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HXU22058.1"
                     /translation="MKLSTDRILTTHVGSLPRPQEVVDLLFAQDRGEEIDEAQFNETM
                     RRSAADLVKRQAEAGIDIVSDGETSKISYATYIRHRLTGFEGDSARPTPQDLDDYPEY
                     RDRLVKAGHSATYKRPVCKGPIKVKNLKPVEQDIAHMKEALSKTKVTEGFMNSVSPGT
                     IAVFQPNEYYPSHEKYLAALADAMREEYEMIVKSGLLLQLDCPDLAMGRHTRFKNLTD
                     EEFLRYAELQVEALNHALANVPADRVRLHVCWGNYEGTHIHDMDCRKIFPIAIKVKPQ
                     ALLIEGANPRHEHEWEAWNTIKLPDDKILIPGVIASSSNYVEHPEVVAQRLLRYANIV
                     GRERIMAGSDCGFGTFAGFGPVFPAFSWMKFRSLAEGARIASKKLWGRAN"
     gene            complement(2375..3712)
                     /locus_tag="VN788_15860"
     CDS             complement(2375..3712)
                     /locus_tag="VN788_15860"
                     /inference="COORDINATES: protein motif:HMM:NF024758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing serine
                     protease family protein"
                     /protein_id="HXU22059.1"
                     /translation="MSPLKRHSALSIAGWLVALVVAIATFVVSGQNRLRNLSARVLGG
                     DGSEVARDGALSSVEIFQKASPSVFVVEALGDDGESLDLGSGVAIAPNLLITNCHVVQ
                     NGSFLRISRAHKKWAARLVQALPNRDLCGLRPVRLGLEPVNVRPSSTLETGESVYAIG
                     APEGLELTFSQGVISGLRETGGVHMVQTSAPTSPGSSGGGLFDAKGNLVGITTFQLRQ
                     GQSLNFAMPGEWINDLLTHPNNAAGATSPAVNDAALESAAWIEIGIEAVNQKNYEVAE
                     SALLKATRMQQPDAYRAWYELARVYGSQQHSEAQALALEQAIRLKPNYADAWRELARN
                     YSMQKDFDKAIAAAKRLTELDARDKENWQLLGFIYLGSNSYAKAIEADEQGLRIAPSD
                     EILLADLGMAYGKQGDREQVLRIYSQLKEKDPLAAKVLFNEYVAPRAIASHSR"
BASE COUNT          803 a         1169 c          973 g          805 t
ORIGIN      
        1 gtgcggcagc gtgatgcacg aagtaatacc atgcgctacg ttccatcgcg gcccgatctg
       61 atgcccgagc aggtgcgaaa ttccaaagcg cgtgttcatc gaaccgaaaa tcgagaacca
      121 cgctgcaaat tggcaatagc cgcgatgcgc cagccgttcg gcgccgctag tcttgatcga
      181 acgaggcaaa tgctccacca aaagtgcgat cgctttcgca ccgagcgcgt cgctcaacgg
      241 gtgatggcgt atcgcgtaaa tcgtctcgat agcatgatcc agcgcgcgca tccctgtggc
      301 gatccacaac cattccggcg tctccagcgt taattcagga tcgttaatca ccgtgcgcgg
      361 ctgaagacgt ggatcggaaa caccggactt tacgagcgtc ttctcgtcgg taactccccc
      421 agcgtgggta tattcgcctg ctgatagggt ggtgggaatc gcgatctgaa ttatttcccg
      481 cttagggaga aatgcgtggc tggcaacttt gcacgcatcg atcgggctgc cgccgccgaa
      541 actgatcagg caatccgctt cgacgcgttc tatttcccgc tggagatgat caaccacgct
      601 gcggggcaca tgttgtcgcg cccctttgaa cactgcagcg catcgcgccc ccgccggagc
      661 agtaacttta gtcaacaacg gcgacgcacc cagactcttt cccgtgacca cgatagcgcg
      721 cttcaatcca cggcgatcaa gttcctcacc cagagccgcc actttgcccg gcccggagat
      781 cacccgctcc agccggctca aattgaaatc aacatttgga gcttccatcg cgcggtgagt
      841 ataccgcatc ggcttcttca cggcctctct cacctcctgt tttttcattc cccaactgca
      901 cacgcggacc cgacacgcct ttcggatgga tcgggcagct ttgctttccg gttgcaactg
      961 gacgccccag cggggtattt tcactgccgc cctaaataca gcgtctacaa ggactcgaaa
     1021 tgaaactcag caccgatcgc attcttacca ctcacgtcgg cagcctgccg cgaccgcagg
     1081 aagtcgtcga tctgcttttc gcgcaggatc gcggcgagga aatcgacgag gctcaattca
     1141 acgaaaccat gcgacgctcc gctgcggatc ttgtgaagcg ccaggccgag gccgggatcg
     1201 acattgtcag cgacggcgag accagcaaaa tcagctacgc cacctacatc cgtcacaggc
     1261 tcaccggttt cgaaggcgat tcggcccgcc ccactcccca ggatctcgac gactatcccg
     1321 aataccgcga tcgcttggtg aaagccggcc attccgccac ttacaaacgc cctgtctgca
     1381 agggtccgat caaagtgaag aatctgaagc ccgtcgagca agacatcgcc cacatgaagg
     1441 aagccttgtc caaaaccaag gtgacggaag gcttcatgaa ttccgtctct cccggcacca
     1501 tcgcggtctt tcagcccaat gagtactatc cctcgcacga aaagtacctg gccgcgctag
     1561 cggacgccat gcgcgaagaa tacgagatga tcgtgaaatc cggtttgctc ctgcaactcg
     1621 attgtcccga cctcgccatg ggacgccaca cgcgtttcaa gaatctcacc gacgaagaat
     1681 tcctgcgcta tgccgagctt caagtcgaag cgctcaatca cgcgctcgca aacgtaccgg
     1741 ccgatcgcgt caggcttcat gtctgctggg gcaattacga gggcacccac atccacgata
     1801 tggactgcag aaaaatattt cccatcgcca tcaaagtgaa gccacaggcc ctgttgatcg
     1861 aaggcgcgaa cccgcgccac gagcacgaat gggaggcctg gaacaccatc aagctccccg
     1921 acgacaaaat cctgattccc ggagtgattg cgtccagcag caattacgtc gagcatcctg
     1981 aggtcgttgc gcagcgcctt cttcgttacg ccaacatcgt gggacgcgaa cgcatcatgg
     2041 cgggctcgga ttgcggtttt ggcacctttg ccggcttcgg tccagtcttc ccggcgttct
     2101 cttggatgaa attccgctcg ctagccgaag gcgcgcgcat cgcttcaaaa aaactctggg
     2161 gccgcgccaa ttagttgaat gagagaggaa agcttctgct acttcttcac tcttgcattt
     2221 cgcccgccca aactcgcggg gcgtcattct gagcggagtc ccgcgcgttt ctctttcgcg
     2281 cggtgtgtgc gcgcgccgga cgcagtcgaa gaatctctct tcgatgtaac attcggacga
     2341 aatgcgggcc ggtcgcatcg cgttccctct tgagttacct agaatgggac gctatcgctc
     2401 gcggtgcgac gtattcgtta aacagcacct tggctgcaag agggtctttt tcttttagtt
     2461 ggctgtatat ccgtaagact tgctcccgat cgccttgttt cccgtacgcc atgcccagat
     2521 cagcaagcag gatttcatcg ctgggcgcaa tccgtagccc ctgctcatcg gcctcgattg
     2581 ctttcgcata agagttgctg ccgaggtata taaatcccag gagctgccag ttttccttgt
     2641 cgcgagcatc gagctcggtt aagcgtttgg cggcggcgat ggccttgtcg aagtccttct
     2701 gcatggaata gttccgcgcc aattcgcgcc aagcatccgc atagttcggt ttcaatcgga
     2761 ttgcctgctc caaggcgaga gcttgggctt ctgaatgttg ttgggagccg tacactctgg
     2821 ccagctcgta ccatgcccgg tacgcgtccg gctgctgcat gcgcgtcgcc ttgagcagag
     2881 cgctttcggc gacctcgtaa ttcttctggt tgaccgcttc gattccgatt tcaatccatg
     2941 ccgccgattc gagcgccgcg tcgttcacag cgggcgaagt ggcgcccgcg gcattgtttg
     3001 gatgcgtcaa caggtcattt atccattcac cgggcatggc aaagttcagg ctctgccctt
     3061 gcctgagttg gaatgtggtt atacccacaa gatttccctt tgcgtcgaaa agccctccgc
     3121 cactcgagcc tggcgacgtc ggagcgctcg tctgaaccat gtgcactccg ccagtttcac
     3181 gtagtccaga gataacccct tgggaaaagg tcagctccaa gccttccggc gctccaattg
     3241 cgtaaacgct ctctcccgtt tcaagcgttg aggacggccg gacattcacc ggctccagac
     3301 ccaatcggac tggccgaagt ccgcaaagat cgcgattcgg cagcgcctgt accaacctcg
     3361 ccgcccattt cttatgggcc cggcttatcc ttaagaacga accgttctga acaacatggc
     3421 aattcgtgat gagcaggtta ggggcgatcg cgaccccgct tcccaagtcc agcgactctc
     3481 cgtcatctcc taacgcctca acaacaaaca cggacggaga agctttttga aagatttcca
     3541 cggaagagag tgcgccgtcc ctcgcaacct cggatccatc gccgcccaga acccgagcgg
     3601 aaagatttcg aaggcgattt tgaccggata ctacaaacgt cgcgatcgcc acaaccaggg
     3661 caaccaacca ccctgctatc gataacgcgc tgtgtctctt gagtggtgac atacttaatc
     3721 gcaattcccg cacaaaaaga cgctaatcct
//
LOCUS       DATNDT010000483         5575 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1611580, whole genome shotgun sequence.
ACCESSION   DATNDT010000483 DATNDT010000000
VERSION     DATNDT010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5575
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1611580"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VN788_15865"
     CDS             complement(<1..761)
                     /locus_tag="VN788_15865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HXU22060.1"
                     /translation="MLLAILEDLDTGVANLSREGIILYANRRFADLLGVSVHATAIGS
                     SVQQYISAASWNSLNSALATAIEAPIRGEMKINVAGETRVVRLSLTPIFGTDGATTIR
                     VVASEVTELLKASQELKDSRASLYSLSARLLQSQDEERRRIARDLHDITGQELAVLAI
                     SLEVAARNANFPEKAREALNESVGLVRKVEDEIRTLSYVLHPPLLDELGLGSALNWYA
                     EGFQKRTGIRVELRIAPNLPRFSPSDETTLFRVVQ"
     gene            complement(1081..3708)
                     /locus_tag="VN788_15870"
     CDS             complement(1081..3708)
                     /locus_tag="VN788_15870"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheB"
                     /protein_id="HXU22061.1"
                     /translation="MKPKLTPEKRRPSSKTKSAVQPIPAQEFLIVAVGASAGGIEAFS
                     ELMKHLPPDTGMAFVLIQHLDPTHHSMLADLLSKITAMSVYEVANGMQVEPNNVYVIP
                     PNTVMSISQQKLQLVPREDSAGVPMPIDHFMRSLSEMGNRAVGAVLSGSGSDGTLGLC
                     EIQAQGGVTFAQDSESAKHDSMPRSAVSAGYADYVLPPKEIARELARLARHPYVSRND
                     DWHPTVEKGSSGLDSGLNGIFQLLRKTTGVDFAHYRQSTILRRIHRRMVINRMDSLRD
                     YLKFVQKSPTEPDALYQDLLINVTSFFRNPRVFDTLKAEVFPQLLKNRAPESAIRIWT
                     PGCASGEETYSIAIALLEFLGDKASETPFQLFGTDVSEASVVKARGGSYSDNIQGDIS
                     PERLRRFFNRSDTGYRISKTIRDRCIFAQHNVLNDPPFSQMDLVCCRNLLIYLESSLQ
                     ARAISIFHYALRPSGYLLLGSSEGIGAATGMFAPEGRDTKIFRKKSVGHQLATFSVDR
                     QSDDSSHSLRVHATPVSPAWSPVEVQKEFDRRLLTNYAPAAVFINDDFDILHSRGNIG
                     LYLKIAPGRASHSILKMAREGLLFDLRSAIARAKKENATVQRHNIQIKGGNGNAAANS
                     ARFVNLEVTPVSVPNLKEQLYMVIFQESHKETDRETSPRRATSQKESRASLGRITKLE
                     GELAATREYLQSLIETQEAMNEELQSANEEILSSNEELQSTNEELETAKEELQSANEE
                     LSTVNDELRSRNLEVNQINNDLNNLLNSTDIAVIMVGNDLTIRRFTPRAQQIYGLIPG
                     DIGRPFFNVNPTIEIPPLQPMVLQVMSNLQTIEEEFTDSRGARYQLKILPYRADDRID
                     GAVITLVDVSALANVASGQ"
     gene            4026..5390
                     /locus_tag="VN788_15875"
     CDS             4026..5390
                     /locus_tag="VN788_15875"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU22062.1"
                     /translation="MATEARALTIGDLIDQHPLSNFQIWTIALCGIVLTLDGFDSLTV
                     NFLATSIAGSTGIPVHSFGPIFSASLFGLMIAALATGPIADRLGRKWPVVFATLSFAV
                     FSLLTPRATTYREFLILRFLTGLGLGGAMPNVVALASEYSPKRLISVVVSILFCGMPL
                     GGAICGLLSAEMIRTWGWHWVFYIGGIIPLVIAILLISMLPESIQFLAVRGKDPEKIR
                     RTLAKVAPEFGSTQAEIVVPHDEQRRKGVPVKYLFTEGRALGTILLWFPYFINLMLIY
                     FISSWLPALLNEAGMTASAAVIATTFFDLGGLLACAGEGFLMNRFGAARMLIYEYALA
                     AVLVGALGVLPRRVTLVSFLAFASGFVVIGAQAGLNALAARFYPTSVRSTGVGWALGV
                     GRLGSFVGPLVGGMLLGIGWSSRGLLLAAAVFSVSAWLAIVLSNLVREHVTPYSRSLD
                     AGGS"
BASE COUNT         1149 a         1408 c         1775 g         1243 t
ORIGIN      
        1 tcctgcacca cgcgaaagag agtggtctcg tcgctcggcg aaaagcgcgg cagattcgga
       61 gcgatccgca gctccacgcg gatgcctgtt cgcttctgaa atccttccgc gtaccaattc
      121 agcgccgagc cgaggcccaa ctcgtcgagc aacggaggat gcagtacata agagagagtg
      181 cgaatttcat cttcgacctt gcgaaccaag ccgactgatt cgttgagggc ttcgcgggct
      241 ttttccggaa aattggcatt ccttgccgcg acctcgaggg atatagcgag gactgcgagc
      301 tcctggccgg tgatatcgtg caagtcacgg gcgatccggc gccgctcctc atcttgcgac
      361 tgcaaaagcc gggcggacaa ggaatagagg gatgccctgg agtctttaag ttcctggctg
      421 gcttttaaca actcggtgac ttcggaggcg acaacgcgaa tcgtcgtcgc accgtccgtc
      481 ccgaagatag gagtgagcga tagccgcaca acacgcgttt cgccagctac attgatcttc
      541 atctcaccac gaatgggcgc ttcgatggcc gtggccagcg cggagttcag cgagttccaa
      601 ctagcagcag aaatgtactg ttgaacggaa gatccgatgg cagtggcgtg cacggagaca
      661 cccaagagat cggcaaaacg gcggttggcg tagaggatga ttccttcacg ggaaagattt
      721 gcgacgcctg tgtccagatc ttcgaggatg gccaggagca tcttgtgatc caagatcgaa
      781 gtttcggcac cgggatccga gccgtttcgc gtggaatggc gaagcgattt cggcaaggcg
      841 ctttctgggg gccttttctt tcgcacaggg cgaccttcta gatggggact ttagtataca
      901 gcagggagaa tagcaatgag cctgtggcta attggggctg cccgaggtgg ttgcctgcgc
      961 agcgaacagg gagccaagca tgtttggcgg gcacgatgta tcgtgcccct gcgagtgcaa
     1021 cggcaaagag ccggcgggac gccggcatta cgacggcaaa ggcggcccat ggatgccaca
     1081 ttattggccg gaggctacgt ttgcgagggc ggagacgtcg accagggtga ttacggcgcc
     1141 gtcgattctg tcgtcagcgc gataggggag gattttgagc tggtaacgag cgccgcggct
     1201 atcggtgaac tcttcctcga tcgtttggag gttggacata acctgcaata ccatgggctg
     1261 gagcggcggg atctcgatgg tgggattgac gttgaaaaaa ggccggccga tatctcccgg
     1321 tatcagccca taaatttgtt gagcacgagg agtaaagcgg cgaatggtca ggtcgttgcc
     1381 aaccatgatg acggcgatat cggtggagtt cagcagattg ttcagatcat tgttgatctg
     1441 gttgacctcg aggttgcgac tgcgaagttc gtcattgacc gtgctcaact cttcattggc
     1501 ggattgcaat tcttccttgg ctgtttcgag ttcttcattg gtgctttgaa gctcctcgtt
     1561 gctcgaaaga atttcctcgt tggcggattg caactcctcg ttcatggctt cctgggtttc
     1621 gataagcgac tggagatatt ctctggtagc ggcgagctcg ccctccagct tggttatacg
     1681 tccgagggag gcgcgtgatt ccttctgcga cgtggcgcgg cgcggcgacg tttcgcgatc
     1741 ggtctcctta tgagattcct gaaagataac catgtagagc tgctccttca ggttgggcac
     1801 ggagacagga gtaacctcga ggttcacaaa gcgggcggaa ttagcggcgg cgtttccatt
     1861 gccaccctta atttgaatgt tgtgcctttg aaccgtggca ttctcttttt ttgcgcgggc
     1921 aatggcactg cgaaggtcga acaagagacc ttcgcgggcc attttgagaa tgctatggct
     1981 ggctcggcca ggagcaatct tcagatagag gccaatattc ccccggctgt gcaagatatc
     2041 gaaatcgtcg ttgataaaca cagcggcggg cgcgtaattg gtcagcagcc ggcgatcaaa
     2101 ttccttttga acctcaacag ggctccatgc cgggctcacc ggtgtagcat gaacccggag
     2161 gctgtgggac gagtcgtccg actgacggtc gacggaaaaa gtggccaact ggtggccgac
     2221 cgatttcttt ctgaagattt tcgtgtcccg accctcgggg gcgaacatgc cagtggccgc
     2281 gccgattcct tcggaagagc cgagtagcag atagcctgac gggcggagcg cgtaatggaa
     2341 gatagaaatg gctctggcct ggagagagga ctcgagataa atgaggagat tgcggcagca
     2401 caccagatcc atttgggaaa aaggcggatc gttcagaaca ttgtgctggg caaagatgca
     2461 gcggtcccga atggttttgc tgatgcggta gcctgtgtcg gagcgattga agaatcggcg
     2521 caagcgctcc ggagaaatat ccccctgaat attatccgaa tagctgccgc cgcgagcctt
     2581 gacgacgctg gcttcgctta cgtcggttcc aaagagctga aaaggagttt cggaagcttt
     2641 gtcgccgagg aattccagaa gggcaatcgc aatcgaatag gtttcctcgc cggaagcgca
     2701 gccgggcgtc cagatccgaa tcgccgattc gggcgctcga tttttcaaaa gctgaggaaa
     2761 aacttcggcc ttcagagtgt caaaaacgcg cggattgcgg aagaagctgg ttacattgat
     2821 caacaagtcc tgatagagag catctggctc agtaggagac ttttgaacga atttcaggta
     2881 gtcgcgcaga ctatccatcc tgttgatcac cattcgccgg tggattcttc gcaaaatcgt
     2941 ggattgtctg taatgagcga agtccactcc ggtggttttc ctcaggagct gaaaaatacc
     3001 gttcaagccc gagtccaggc ctgacgaacc tttctcgacg gtgggatgcc agtcgtcatt
     3061 gcgggagaca taaggatggc gggcaagccg cgcaagttcg cgcgcaattt ctttcggcgg
     3121 gagcacgtag tcggcgtacc cggcggatac ggcgctgcgg ggcatgctgt catgtttcgc
     3181 ggattccgaa tcctgcgcga aggtgacgcc gccttgcgct tgaatttcgc aaaggccgag
     3241 cgtaccgtcc gatccggaac cggagagaac agcaccaaca gcgcgattcc ccatctcgga
     3301 aagagacctc ataaaatgat ctatcggcat gggcactcca gccgaatcct cgcgtgggac
     3361 gagctggagc ttctgctggg aaatggacat gacggtattg ggaggaatga cgtaaacatt
     3421 gttgggctcg acttgcatcc cattcgccac ttcgtacacg gacatggcag tgatcttgga
     3481 gaggagatcg gcaagcatac tgtgatgagt cgggtccaaa tgctggatca ggacgaacgc
     3541 catgccggta tcgggcggca aatgcttcat tagctcgctg aaggcctcga tacctcctgc
     3601 ggaagcgcct acggcgacga tgaggaactc ctgggcaggg atgggctgta ccgcagactt
     3661 cgtctttgat gaaggccggc gtttttctgg agtcaacttg ggtttcatgg tggcctcgcg
     3721 caaggcgcgc tctgcaacgc agcggccggg aggaacgcgc cctaacaacc agcggcgcgg
     3781 tgcattcgcg gccgcgttac gctaacgcat aaaccactga tcgagagcgg agtctagtcc
     3841 ttttccggcg ccgggggcaa ccagttgtac aggtaaggcg ataatgccac cgtcgcgcag
     3901 cctacaaagg cgacgatggg aagtgcgctc gtcgtgccta ctttcccggc gaagaggtgt
     3961 acactcgggg ccgaactggc cggatatttt gaggctgtgg ttgtaacgaa attgcggagg
     4021 ctagcgtggc aacggaagcg cgggcgctga caatcgggga tctgattgac cagcatccgc
     4081 tgagcaattt tcaaatctgg acgatcgccc tctgcggaat cgtcctgaca ttggacggat
     4141 tcgattcgct taccgtgaat tttctggcga cgtcgattgc gggcagcacg gggattcccg
     4201 ttcatagctt tggaccgatt ttctcggcga gcctatttgg cttgatgatc gcggcgctgg
     4261 ccactggacc tatcgcggac cggttggggc ggaagtggcc ggtagtcttc gcgacgctga
     4321 gcttcgcagt gttttccttg ctgacgccgc gggcgaccac ctatcgcgaa ttcctaattt
     4381 tgaggttcct cacagggcta gggctgggcg gtgcgatgcc caacgtggtc gcgctggctt
     4441 cggagtattc gccgaagcgg ctgatctccg tggtggtgtc gatattattc tgcggaatgc
     4501 cgctgggcgg agcgatttgc ggattgctga gcgcggagat gattcgcacg tggggatggc
     4561 actgggtatt ttatattggc gggattattc cgctggtaat cgcaatcttg ctgatttcga
     4621 tgctgccgga atcaatacaa tttttggcgg tgcgcggcaa ggacccggag aaaatacgga
     4681 ggacgctggc gaaggtggcc cccgaattcg gttccacgca ggccgagatc gttgttccgc
     4741 acgatgaaca gcgccgcaaa ggcgtgccgg tgaaatacct gttcacggaa ggacgtgcgc
     4801 ttgggacgat tctgttgtgg tttccctact tcattaactt gatgctcatc tatttcatca
     4861 gcagctggct gcccgcgctt ctgaacgagg cggggatgac ggcatcggcg gccgtaatcg
     4921 ccaccacgtt cttcgatttg ggcggcttgc tcgcgtgtgc gggcgaggga ttccttatga
     4981 accgattcgg cgccgcccga atgctcatct acgaatacgc gctggcagcg gtattggtgg
     5041 gcgcgctggg tgtactccca aggcgcgtaa cgctcgtttc attcctggcg ttcgcttcgg
     5101 ggttcgtggt gatcggagcc caggcggggc tgaatgcgct tgcagcgcga ttctatccta
     5161 cgtctgtgcg gtcaaccggc gtaggttggg cgttgggagt agggcgcttg gggtcattcg
     5221 tggggcctct ggtgggcgga atgttgctgg gaattggatg gagttcgcga gggctgctac
     5281 tcgcggcagc agttttttcg gtgtccgcgt ggctagcgat cgtgctgagc aatttagtgc
     5341 gcgagcacgt gacaccgtac agccgttcgc tcgatgcggg cggatcttga ttgcgagacg
     5401 gctttagctg gagcgcggac tgactcggca aaatggggca aaccgaggga ggcgaccatg
     5461 cgtcttcttc tggtagaaga cgaaagaaaa gtggctagct ttatcgcgcg ttcgctgcgc
     5521 gagaatctgt acaccgtgga tgtggcggac acgggcgaaa aggcgctcga gatgg
//
LOCUS       DATNDT010000484         4874 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_707014, whole genome shotgun sequence.
ACCESSION   DATNDT010000484 DATNDT010000000
VERSION     DATNDT010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707014"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2134
                     /locus_tag="VN788_15880"
     CDS             <1..2134
                     /locus_tag="VN788_15880"
                     /inference="COORDINATES: protein motif:HMM:NF017183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HXU22063.1"
                     /translation="MVKCGGFSLLFVALLVACIGIGGCSSAPGPLKITSTSLPAGNFG
                     NAYTATVNATGGVPPFMWSIASGSLTAGLSLGSSTTRSVTISGTPTAQIDSNFSIAVT
                     DSKGTSATQTLTIAIGAPLPLAVTTTTLPGGVPNQVYPPTTLQASGGVPPFTWIITSG
                     NLPAGLSLASTGIISGTPTAVGTANFTVQVADSEATPMTATANLSITVGDLGVLSGNY
                     AFEFSGFNAGGQVVVVGSFTADGAGNLTNGVEDFNTMAGPPKNQTFTGTYTLGADKRG
                     LLVFSSLTGSPTYSFAIDSTGAHGRLIEFDSSGIRGSGQLERRTVNTCAFNTLTGNYA
                     FGVTGQKAAVGGFAAGPSVVVGSFLAAPATSQGGQGAISSGEADSSDPSGVTGGTGIG
                     GLSGNFMAFASAPQNTRCTLTLSPVNINVTSMTFSVYPVSSKEAFLIETDTVSSTTPF
                     LTAGKMLAQTGSPFSGVAGNTFTSTSVAGMVGQFPSGSAAYVPDLALVSLTGTSSSAY
                     NISILENRAGTVMPPFNPGSLNFVTSDQFGRVDSGISTPISLVFYIINQNEAFAIGES
                     LSSSTPVPFFGIFEPQSPSPLNLNASDLNSTFVLGTSEPASAPVTDISGSIVLANTSA
                     TSGTIAGTQDQSASGGNSSAQIVTGTFAGLSSTTGNGTLALTAPAAFTGDFLVVSPTK
                     IMVLSTTPSDAEPVLLFLGNCETTCGED"
     gene            complement(2176..3060)
                     /locus_tag="VN788_15885"
     CDS             complement(2176..3060)
                     /locus_tag="VN788_15885"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HXU22064.1"
                     /translation="MTALHRECLMSAEPIKTIAVLGAGTMGNGIAHAFARAGYAVILR
                     DVETRFLDRGIATIAKNLDREIKKEKITEAEKNAVLARLRPTSDIGTLAAADFAVEAV
                     PEQRELKIRVLQEVDVVLRSSAILASNTSSISITELAEQTRRPDRFIGMHFMNPVPMM
                     VLVEVIRGRATSDATFQTTMELCEKLGKKPVAVNDAPGFVANRVLMPMINEAAYAVME
                     RVATPEAVDAVMKLGMNHPMGPLELADLIGLDVCVEILGVMAKGFDDAKYRPCPLLQE
                     YVAAGRLGRKSGQGFYQY"
     gene            complement(3057..3401)
                     /locus_tag="VN788_15890"
     CDS             complement(3057..3401)
                     /locus_tag="VN788_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22065.1"
                     /translation="MICPKCKVEYRDGFTKCSDCDVALVDRLDPSKPSAPSKEERPAL
                     AWRGDDPVVFSAVLAALGDEEIPNYEMSDHEQLPFEPAIPRPQHAIFVRQEDLSRAKK
                     AIQDVIGPDAGE"
     gene            complement(3429..4616)
                     /locus_tag="VN788_15895"
     CDS             complement(3429..4616)
                     /locus_tag="VN788_15895"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014100339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HXU22066.1"
                     /translation="MEKAVILSAVRTAIGKFMGGLSQLTAPELGAKVIAEAVRRAGID
                     PKQVDEAIMGNVLQAGLGQNPARQAALKGGLDPRVAAMTINKVCGSGLKAVALAAQAV
                     MLGESEIVVAGGMESMTNAPYLMKGARTGFRLGNSELLDSMIVDGLWDAYENFHMGMT
                     AELVAEKYGISRLEQDEFALESHRKAVRATKSCFVSEQIVPIELPQRKGPPLIIKTDE
                     SPREDTSLEALGKLKPAFKKEGGTVTAGNAPGTNDGAAAVVVTGEGTAARLGRPVMAR
                     IVAQAVSGVEPKWVMMAPVEAVEKLLAKTGWDRDKDVDLYELNEAFAVAAIAVTRQLK
                     LDPEKVNVNGGAVALGHPIGASGARILVALLYELQRRNLKRGIAALCLGGGNAVALAV
                     ERP"
BASE COUNT          963 a         1577 c         1261 g         1073 t
ORIGIN      
        1 catggtgaag tgcggcgggt tctcattgct attcgttgcc ctgctcgtgg catgcatcgg
       61 tattggagga tgcagcagcg cgccggggcc gctgaaaata acatccacca gcctgcccgc
      121 cggaaatttc ggcaatgcgt atacagccac tgtgaacgcc acaggcggcg tcccgccctt
      181 catgtggtcc atcgcgtcgg gttcgctcac agccggactc tcgcttggaa gcagcaccac
      241 gcgctccgta acaatttccg gcacgcccac ggctcagatc gattccaact tctctatcgc
      301 ggtgaccgac tcaaagggca cttccgccac gcagacgttg acaattgcta tcggcgcacc
      361 attgcccctc gcggtaacaa ccaccaccct tccgggtggc gttccaaatc aagtttatcc
      421 gccgactact ttgcaggctt ctggcggcgt tcctccgttc acgtggataa taacttcggg
      481 caacttgcct gctggacttt ctttggcttc gacaggaatc atcagcggca cgccgacagc
      541 tgtaggcacc gcaaacttta ccgttcaggt cgccgattct gaagcaactc cgatgaccgc
      601 cactgcaaac ctgagtatca cggtgggcga cctcggcgtg ctaagtggca actatgcttt
      661 tgagttcagc gggttcaatg ccggcggaca agtggtcgtc gtcgggagct tcacagccga
      721 tggcgcaggc aatctaacga acggcgtgga ggatttcaat accatggccg gcccgccgaa
      781 gaatcagact tttacgggca cctacacgct cggcgctgat aagcgcggcc tactggtttt
      841 cagcagcctc accggatcgc cgacctattc gttcgcgatc gattccaccg gtgcgcacgg
      901 gcggttgatc gaattcgact cgagcggtat tcgcggatcg ggccaactcg agcggcgcac
      961 ggtcaacact tgcgccttca acacactcac cggaaactat gccttcggcg tcaccggtca
     1021 gaaagcggct gtcggaggct tcgcggccgg gccttccgta gttgtgggca gttttttggc
     1081 agccccggca acctcgcagg gaggccaagg cgccatttct tccggcgaag cggattccag
     1141 cgatccgagt ggcgtgacgg ggggcaccgg aattggaggc ctctccggga actttatggc
     1201 gttcgcatcc gctccacaaa acacgcgatg cacgctgacg ctttcgcccg taaacatcaa
     1261 cgtgaccagt atgaccttca gcgtctaccc cgtatccagc aaagaagcat tcctcattga
     1321 aaccgatacc gtcagttcca cgactccttt tctcactgcc ggcaagatgc ttgcgcaaac
     1381 tggatcgcca ttttcgggcg tggcgggaaa tactttcacg tcgacaagcg tagccggtat
     1441 ggtcggccag ttcccttcgg gaagcgccgc ttacgtgccg gatctcgcgc ttgtctcgct
     1501 cacaggcacc agcagcagcg cctacaacat ttccatactc gaaaaccgag cggggaccgt
     1561 gatgccaccg ttcaacccag ggagcctcaa cttcgtgacg tccgatcagt tcggacgagt
     1621 cgattcagga atttccactc cgatatcgct ggtcttttac atcatcaatc aaaacgaggc
     1681 cttcgcaatt ggtgaatcgc tcagcagcag cacgcctgtt ccgtttttcg ggatttttga
     1741 gccgcagtca ccctctccgc tcaatcttaa tgcttcagat ctcaattcca cgtttgttct
     1801 gggcacttcg gaaccagcca gcgcccccgt tactgacatt tctggctcga tagtgctggc
     1861 gaatacgtcg gccacatctg ggacgattgc cggcacccag gaccagagtg cgtccggggg
     1921 caactcctcg gcgcaaatcg taaccggtac tttcgccggc cttagctcga ccaccggaaa
     1981 tggcacgctt gcgctgaccg cacccgcagc tttcactgga gatttcctcg tcgtctcacc
     2041 cacgaaaatc atggtgctgt ccacgacacc cagcgatgcg gaaccggttc tgctgttctt
     2101 aggaaactgc gaaactacct gcggggaaga ctagttcaag ctgaagctgc gttcagctca
     2161 cgaccggtgt tcgcgtcaat actgataaaa tccttgcccc gatttgcgtc ctaatcgccc
     2221 ggcagcgacg tattcttgca gtagggggca aggacggtac tttgcatcgt caaatccttt
     2281 cgccatcacg ccgagaattt ccacgcacac gtcgagtccg atcaggtcag ctagttctag
     2341 cgggcccatc ggatggttca tccccagctt catcaccgcg tccacagcct ccggcgtggc
     2401 cacgcgctcc atcaccgcat acgcagcttc gttaatcatg ggcatgagca cgcggttggc
     2461 gacgaagccc ggcgcgtcat tcaccgccac gggttttttc cccagcttct cgcacagttc
     2521 catcgtcgtt tgaaacgtcg catcgctggt cgcgcgccca cggatcactt ccaccagaac
     2581 catcatcggc acaggattca tgaagtgcat gccaataaag cgatccggcc gtcgagtctg
     2641 ctccgcaagc tcggtgattg aaatcgagga ggtgttgctg gcaaggatcg cgctggagcg
     2701 caatactacg tccacttcct gcagcacgcg tattttcaat tcgcgctgtt cgggaacggc
     2761 ttcgaccgca aaatccgcgg cagccagagt tccaatatcg gatgttggcc gtagccgcgc
     2821 caacaccgca tttttttccg cctcggtgat tttctctttc ttgatttcgc gatccagatt
     2881 cttggcaatc gttgcgatgc cacggtccag gaatcgcgtt tcaacatcgc gcagaatcac
     2941 ggcatagccc gcgcgggcga acgcgtgggc gatgccattt cccattgtgc ccgcacccag
     3001 cacggcgatt gttttgattg gctcagcgga catcaggcat tcacgatgga gagcagtcat
     3061 tcgcccgcgt caggcccgat tacatcctga atcgccttct tcgcgcgcga caaatcttcc
     3121 tgccgcacaa aaatagcgtg ttgtggccgg ggaatggccg gctcaaaggg gagttgctcg
     3181 tgatcggaca tttcgtaatt cggaatttcc tcatcaccta gagcggccag cacggcgcta
     3241 aacacaaccg ggtcgtctcc tctccacgcc agcgccgggc gctcctcttt cgacggcgcc
     3301 gacggcttcg aaggatcaag cctgtccacg agcgcgacgt cgcaatccga gcatttcgta
     3361 aaaccgtcgc ggtattccac cttgcatttt gggcagatca tgttttttag agtctcagaa
     3421 ttttgctgtt aaggtcgctc gaccgccagc gccacggcat tccctccacc caggcagagc
     3481 gccgcgatgc cgcgcttcaa attccgccgc tgcaattcat aaagcagcgc cactaatatc
     3541 cgagccccgg acgcgccaat cggatgcccc agcgccaccg caccgccgtt gacgttcact
     3601 ttctccggat cgagtttcaa ttgccgcgtc acagcaatcg cggccactgc gaacgcctca
     3661 ttcaactcat agagatcaac atccttgtcg cgatcccacc cggttttcgc cagcaacttt
     3721 tccactgcct ccaccggcgc catcatcacc catttcggct cgacgccgct cacggcctgt
     3781 gccacgatcc gcgccatcac cggcctgcca agccgggcag cggttccttc tccggtgacc
     3841 actactgcgg ctgcgccgtc attcgtgccg ggcgcgttcc ccgcagtcac cgtaccgcct
     3901 tcttttttga acgcaggctt cagcttcccc agtgcttcaa gcgacgtatc ttcgcgcggc
     3961 gattcgtcag tcttaattat cagcggcggc ccttttcgct gcggcagctc gatgggaaca
     4021 atttgctcgc tcacgaagca tgatttcgtc gcgcgcacgg ccttgcgatg gctttcgaga
     4081 gcgaactcat cctgttccag ccgcgatatg ccatattttt cggccaccag ttcggccgtc
     4141 atgcccatgt ggaagttttc gtaggcgtcc cagagtccgt ctacgatcat ggagtccagc
     4201 agctcggaat tgcccaggcg aaaacctgtg cgcgcgcctt tcatcaaata aggcgcgttg
     4261 gtcatcgatt ccatgccgcc cgcaaccaca atttcggatt cgcccagcat caccgcctgc
     4321 gccgcgagcg ccacggcctt caaccccgaa ccgcaaactt tgttgatcgt catcgccgca
     4381 acgcgcggat caaggccgcc tttcaacgcc gcctggcgtg ccggattttg tcccagaccc
     4441 gcctggagga cgttgcccat gatggcctcg tcaacctgtt ttggatcgat tccagctcgc
     4501 cgcacagctt ctgcaatcac cttcgcaccc agctccggcg ccgtgagctg tgaaagtccg
     4561 cccatgaatt tcccgatagc cgtgcgcacc gcgctcaaga tgacagcttt ttccacgcga
     4621 ttccttctct cttagttcta ctaacaactt caagtatagg cgcgacgaaa ccgcccggtc
     4681 aatttgcagc agcgtttcaa cgggcacggt tcgttgcggc ggagcccgat gcacgcattt
     4741 ggctacatgt gacccactaa actcatacag cgggcgcaga attgactctc ccacaaacaa
     4801 aatgtgccct tgtgaatccg cgcgctctcg ccgataattt ccgcccatgg atgaatcgag
     4861 ccgcgttcgt gcgg
//
LOCUS       DATNDT010000485        20155 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_707412, whole genome shotgun sequence.
ACCESSION   DATNDT010000485 DATNDT010000000
VERSION     DATNDT010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 20155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20155
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707412"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..124
                     /locus_tag="VN788_15900"
     tRNA            48..124
                     /locus_tag="VN788_15900"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Arg,seq:ccg)
     gene            complement(184..885)
                     /locus_tag="VN788_15905"
     CDS             complement(184..885)
                     /locus_tag="VN788_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018434267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HXU22067.1"
                     /translation="MAGKNSRVIVRIREMILHGELTPRQRVREVELAAKLGVSRTPVR
                     EALPILAQEGMLTQLDTRGFVVRAFTPREIMDAIDVRGVLEGLAARMLAEQGPPRRLI
                     QSLHECLRDGDEIFAKRHLLESDEARYGEMNKQFHSLIIEGAGSKVIADTIERNDRIP
                     FAAAYAIAFDNVNLPRMYDYLYSAHHQHHLIVQALENGEGGRVAALMYEHAYATKASI
                     NMVRKPLAATSDDNA"
     gene            complement(1454..1816)
                     /locus_tag="VN788_15910"
     CDS             complement(1454..1816)
                     /locus_tag="VN788_15910"
                     /inference="COORDINATES: protein motif:HMM:NF024776.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22068.1"
                     /translation="MRMREVVLRAIAKKITWDQAADILGMSHSAMWRLRGLYQRRGYD
                     GFWVRATRKPGGLPVPLSDLEKVLLLYQQQYSHLDARSFQKILRKEHSIHLDCARLSQ
                     ALREAGLTRAMQLRSERE"
     gene            complement(1970..5557)
                     /locus_tag="VN788_15915"
     CDS             complement(1970..5557)
                     /locus_tag="VN788_15915"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU22069.1"
                     /translation="MSTGSNTTKCVWTGVFLAALVFLFSAGRVQGQAQTATIFGTATD
                     PSGGALAGAHIQVTNVGTNISQSTTTDAQGRYTIAQLPVGTYSVEASLSGFQTVVHKG
                     VTLSVGGTLVVDFSLPVGEVTQTVNVESDVSRVETETSEVSTLISPQQMRDLPLNGRN
                     FEQLITLAPGVSTVGAALNAVTGRLYGMQDNYSVAGSRPTGQMFLLDNTDIRDFWEHG
                     TGSGYAATSLGVEAIGEFQVLTNTYTAQFAGNGAVINATSRSGTNDLHGGAYEFFRNN
                     VLDARDIVDPVTGPPPFRRNQFGGDLGGPIKKNKLFFFGNYEGLRQSLATTTDIFLPE
                     PYTVQGMLPCSLVTPTPGTCTPSTSANSTQAGIPIGAVPGANTTTEQIAALYSLCKGC
                     HLITPTIGAVNTNLPGNCVTIGCDLGGYFETAAAPPLTIEENYYMGRVDYTLGSNDSL
                     FARFTFDNANVGDPRDPYQIFPETDHTRNQFLTITERHVASATLVNSIHFGYVRNNEN
                     SQAQPALTNAQLSAANAFSSSLGGPAITSDPLHFVDTQPGELPRQDGQNGGFQQVIFP
                     IGPDPNRPDEIVQNKFSGGDDITWTHGAHSLKIGAVVTRVQTQNLQTAYSNGGFFLSY
                     GVPAPVYDAQFWLSGTPFLAFAVPTVPALNNATRYFREIMVAPYIQDDWKVSSRLTLN
                     LGFRYDYDTNPVGWAFPHQPMTTLVGSFLPPVGPVAPPGGAPVFTPVRHVFANNVNAG
                     NLEPRFGFAFDPFRDHKTSIRGGVGIFSDPTSGRLWESNFINTAPSGSSFLVFPSFPN
                     LCATGCNPPGAVSEFAGVTYQPRGGPLFESPYQITYNLNLQRELWRSTVLSVGYVGSV
                     SRHLWTQGDINPPKCLTFPDCKALPQIPKSYPTASDATFTVSNACHFPTQTSCYGSGV
                     QFPPPAPFAQTGPRINGAFGSLIQAYNTGASAYNSLQVSLNRQFARNIAGQFNYTWSR
                     CVDDGSFASSLEEFAQLVVDRYNERYAYGNCTFDIRHNISANGLYSLPFKGNRLVEGW
                     QISAIVGIHTGLPLNVYNNTLTSTDPAFLGTQWGSQANYTFAPGCHPNHLLKKRINAT
                     TIQWFDPACYEAQAPGFLGNVKRDSLPGPGTFSADLSVTKNTKITEKLNLQFRTECFN
                     CFNHFNVGGTGAGILGEINAPGGTTGQTTFSQSPVVTPRQIQFALKLDF"
     gene            5898..7298
                     /locus_tag="VN788_15920"
     CDS             5898..7298
                     /locus_tag="VN788_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017181837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HXU22070.1"
                     /translation="MSYKSLEDLLQSVGNPVTLLRNSQTGPYVYPVVPPEYSNWRDEQ
                     RAWQQTCVLFNQSYHMEDMYISGPDATRLLSALGINSFKGFEPGRAKQFVPCNYDGYV
                     IGDVILFYLEKNMFNLVGRPAVHNWVQYNAEFDKYNVTFDRDPRAVAQKGPVVRKAYR
                     YQLQGPNAMKLTEKAMGKPAPELKFFHLTTIPIAGRTVHALRHGMVGQPGWELFGPWE
                     DGEAVKAAIIEAGKEFGLRLVGARAYSSNTLESGWIPSPIPAIYSGEKMKPYRQWLSA
                     NSYEAIASLGGSFYSDNIEDYYLNPWDLGYGPILKFDHEFIGREALEKMAKQPQRKKV
                     TLALDSDDVTRAIHTMFQKKDRAKYFDFPSAVYATLPYDKVMSDGKIAGISTWCGYSS
                     NEGKMLTLAILKEEYAEPGMELTFVWGEEGGGSSKPTVEPHVQTKIRAIVSPVPYSEV
                     ARTQYAEGWRTAQKVS"
     gene            7364..8533
                     /locus_tag="VN788_15925"
     CDS             7364..8533
                     /locus_tag="VN788_15925"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HXU22071.1"
                     /translation="MASSAPHNPPFRADHVGSLLRPTELKNARMDCAAGKISADDLRS
                     LEDAAIRKVVARQEEIGLQSITDGEFRREDFYTDFYVRGLGGVEVRMESTAAYFVDHE
                     GRKIPVPWTQVVSRMQWRKPIYADHFRFLRSVTTQTPKVTVPSPIILHFTCGSAAIRA
                     NAYPDMDLFWTDVIEAYQKEMNSLYEAGCRYLQVDDPPMATLCGENFQAMMKSRGDDP
                     EHAMKVLYPEMINRAFANRPPSLHLAMHLCRGNNQSGWFGEGGYDPIAEVLFNKINVD
                     SYFLEYETDRAGTFEPLRFVPKNKTIVLGLVSSKLPNLESKDLLKRRIEEASKYVDID
                     QLSLSPQCGFASSTEGNRLTEEEQFAKLRLVVEVANEVWGHKKGSSSSHTASANT"
     gene            8602..9000
                     /locus_tag="VN788_15930"
     CDS             8602..9000
                     /locus_tag="VN788_15930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22072.1"
                     /translation="MNRRKLLSLLGVSPVILAGTASAKDRSKKTPSSASSESKIQGLN
                     PRGIPPSIQLIPMAPRPASLDGKTVYLVSDGFPGADHFLNQIAIWFKKNMPSVMTQYR
                     LKKGGFADDDPALWAEIKAKGSAVIMAIGH"
     gene            9034..10341
                     /locus_tag="VN788_15935"
     CDS             9034..10341
                     /locus_tag="VN788_15935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="UGSC family (seleno)protein"
                     /protein_id="HXU22073.1"
                     /translation="MTLEKMGIPTAPMVTIAFKDLAKTNAAGRGMPDERICFTPHPVW
                     GKTDAQMYAYLEGNDPVTGKPLMKEVIGALTNPLTADEKKTGTVTPSVGPPMFVDTPD
                     NLQKYYKDNGMTDYMPIIIPTQERVDAMLKGTSHSPDEVIGELTPARGAFPAWKFTVK
                     HVAISAVMSGADPDYLPTILALAATGLPSLSSSTNSFATAAVINGPIRDKLNMNYGIG
                     AMGPFNDFNASVGRAWTLFSKNCGNCGQAGETYMGTQGNAVNFGNIIIVENEKDSPWK
                     PFHVQKGFKPEDNVVSVFFGYGIAQGQGGKGSAIKPVPAFDQGLVYNFIPLTSIFGAL
                     AVLDPLVAKALVDRGYDTKEKLTAWLQKNSTLTVGDAKTMLFTGGAALRGPHAAELKD
                     DDLIPHWPSPSMINVVVVGGQTNPYYQVANMSYARSVAIDKWM"
     gene            complement(10458..11192)
                     /locus_tag="VN788_15940"
     CDS             complement(10458..11192)
                     /locus_tag="VN788_15940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-CTERM sorting domain-containing protein"
                     /protein_id="HXU22074.1"
                     /translation="MTDCRKIGCVLAVVGLFCASLFLTIAAQADTLLTSAVSITSPSV
                     IDFDAFGPGITFTSGPVDLGSGVTWSSTTTESSIGSGNYFLLSGTDSNGVWDSSLHYV
                     ALGGFPGTMTFTFATPVSAVGAFMNYAPCAVCSDMTIDAYDGSTSLGAFDINLLAPIS
                     TPGGVDAGAFRGISVDSPEITSFTVSNSAVILTDLTFSTPSAGQAPVPEPGTILLFGE
                     GLLGIVALGLNDRFRARARYNSRTSY"
     gene            complement(11459..12358)
                     /locus_tag="VN788_15945"
     CDS             complement(11459..12358)
                     /locus_tag="VN788_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22075.1"
                     /translation="MTAIIRARIAILPILLLLLSTGVFAGAAFGQVDPTGMWGPLFYE
                     DIPERLAGAEIGDYLGLPINAAARLRADSWDATLVELPENQCREHGADYGWRGPSALS
                     IWKEVDKASQKVIAYRTHLSAYGAEQTIWMDGRPHPPDYARHTWEGFSTGHWDGDTLV
                     IETTHLKENWLGLNEVPRSDAATASAHIMKHGDYLTVAAITYDPIYLTEPLIRTSDFI
                     YSPQMEMAPWPCEPVDEVDRPPGVVPSHLPGTNKFLEEFARRYRIPFEATRGGAETMY
                     PEYQLKLKQMKIPPPLPKTAPAH"
     gene            12662..13633
                     /locus_tag="VN788_15950"
     CDS             12662..13633
                     /locus_tag="VN788_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019155376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU22076.1"
                     /translation="MNYPATGYHHITACAGGAQEDVNFFTGIVGQRLAKQTILMDGRF
                     PHYHLYYSNANIEPGSIMTTFPYNRLMGRRGSGQVQSITYTVSKGALPFWQEHFKRHN
                     VDHGPIEERFGQRFIRFTHPVGMTFEVLEDKDDQRIGWSTPEIAQGESVRGFYGTVLS
                     VRDVTEQEKFLTEALGFRKTGVDGKYHRFEIGSGGANKTLILLHEPDRAPGSWTFGAG
                     TVHHIALWVADDEAMAAQKAIYEELGYTDASEIKDRYYFHSMYVRSPGGILVECCCNV
                     PGGFEKDEPAATLGKKLHLPPWFEDRRDEILKTLEPIVAPETATSRA"
     gene            13668..14843
                     /locus_tag="VN788_15955"
     CDS             13668..14843
                     /locus_tag="VN788_15955"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HXU22077.1"
                     /translation="MKRSENRILTTHVGSIVRPQQLRDLADEAAKSPENQKRYEDFLR
                     DSVADVVKLQAKAGIDIVNDGEFGKSSWSNYILERITGFEHRPDQLHPVYWLGRDRDR
                     FPEVMAAEFPWMTKGVATEACIGPIKYRDHANIDRDIRNLKGALKSVNVEEAFLPVVA
                     PASSAYDGVNEYYPTEKEYVYAIADALREEYRAIYEAGLIVQIDDAVLANMYDHYVEQ
                     SPEKYRQWAGLRIEALNHALQGIPEDRVRYHVCFGSWHVPHLADAPLEEIVGFILSVR
                     AGAYSIEAANPRHEHEWRVWEEKKLPAGKILIPGVVTHHTMTVEHPRLVADRILRFAR
                     IVGRENVIAGTDCGFAQAQFIQRAAPSVMWAKFESLVEGARIASAELWQKGKRAQPS"
     gene            14976..15926
                     /locus_tag="VN788_15960"
     CDS             14976..15926
                     /locus_tag="VN788_15960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22078.1"
                     /translation="MKTLAQRVAWLVALMGILAFAQPARAAAAQCNRACLKNTVDKYL
                     AALVAHDPSQVSLAPNVKFVENTVPMHPGDGLWKTASAAPTTFKIYVPDPVSEEVGFL
                     GVMQEKGKPIELALRLKIRNGKIVEAEHLIARNLMVRNLANLKTPRPGLLATVPPDQR
                     VPRDQMLKIGASYYEALVTADANNAPFADDCVRRENGMQTTGNPPPAKPGRGTIGAMG
                     CARQLRTHTFDYIKRIEPRRVEIADPETGLVFGLSQFRQPMKEKTVKIVGVPNVDHVD
                     LNIKPFDLPAAHIFKIRNGKIHEIEAMGFTMPYNSKTGWE"
     gene            16078..16404
                     /locus_tag="VN788_15965"
     CDS             16078..16404
                     /locus_tag="VN788_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22079.1"
                     /translation="MRMLLKISIPVEPGNAAARAGSLESTIKSILDELKPEAAYFAED
                     GGERTGYIFFDMKESSQLPAIAEPWFLAFNAKITVRPAMTPQDLAAGASGMERAAKRY
                     GRAATA"
     gene            16678..17664
                     /locus_tag="VN788_15970"
     CDS             16678..17664
                     /locus_tag="VN788_15970"
                     /inference="COORDINATES: protein motif:HMM:NF014606.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HXU22080.1"
                     /translation="MTATYGQNTDETPQQTNQKIQQLAAQAQAHPTNTPIGSGDLLHV
                     DVFDVPELSRDVRVSDTGEISYPLVPGKIKVAGKTPFQLETEMERLLIENGLVSHPQV
                     SVFVREQTSQPVSVVGAVNHTMVYQVVRPTTLLEVLSAAGGISDTAGDVVIVTRPNNP
                     DVAKVEPASATTKSQPDEQKIIVRIRDLLESGDSVYNIPILGGDTVTVPAAGIVYVLG
                     FGVAQPGGYVLQSHGEQVTVLKALALAHGLTPFAKSNSAVIMRENPATRQRDLIPVHI
                     KELEKNKTADIPLKSNDILYVPDSAGKKALAKGSEAALGIGTSLAVYRVAYH"
     gene            17714..20011
                     /locus_tag="VN788_15975"
     CDS             17714..20011
                     /locus_tag="VN788_15975"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HXU22081.1"
                     /translation="MEDASRYAIRKPAEQLAVITPPRNLAAWEQMPREAHLLDYLIIL
                     RKHQWLILTFLLTVVTVVTIATFKMKPVYEAAARVEVDREAQNELPFQPTNSYDEYAD
                     MDTYIETQTKILQSETLALQTITSMDLTQYPEFGGNPAAGNFGRGGSAIKRPAILGAF
                     LGSLSVVRVPNSRLIEVRFAAQNPQLAAQVVNAHLQNYIEQNFRSKYDATTQASTWLS
                     SELEELRIRVEKSEDARLAYERQNQIWQIDEKQDITTQKLGDLSRAATAAQMDVAQKE
                     ALYRMATSGNVDALPAALSNGVVQDLLRKKSQLDEDYAQAINQYGPNFPKVLRLAAQQ
                     KQVDADLANARKTMVESVEEDYNTARSHAALLQDALDKQKADANDLAEKLVQYHILQH
                     DADSNKQLYDGLLQKLKEAGITAGLRSSNIRIVDPALVPTTPSRPRKARNILLALIVG
                     LVGGIGLAIFREYLDNTVKSPTDIENLTGLPSLAVVPFLPALNGHHGRLSRIAREATG
                     LAASSPHVELLSYVQPKSQISEAFRALRTSLLLSQAEHPPQVILVTSALPREGKTTAA
                     VNLAVTLAQLGDRTLVMDADLRKPGIRRALNLTSGRDIGLSSYLAGVSSLDEVTTSHP
                     AISNLAALTTGPVPPSPADLLSSHRMRETIADLRRRFKFIVIDSPPILAATDAVIISS
                     LTDGVLLVVRSGATPKEAFTRSRDLLGAVKSRLLGVVLNAVDSSAPDYYYSYRYYPYA
                     YGYGYSEEGGKPPESFGGLDHTPES"
     gene            20027..>20155
                     /locus_tag="VN788_15980"
     CDS             20027..>20155
                     /locus_tag="VN788_15980"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HXU22082.1"
                     /translation="MRHLITGGAGFIGSHLSEALLARGDEVFIVDDLSTGSVENIRH"
BASE COUNT         4559 a         5679 c         5631 g         4286 t
ORIGIN      
        1 ttggtcagcg cgctaaaagc aggtaaaatc gcgatgctct ctcatgcgcg cccgtagctc
       61 agttggatag agcgtgtgcc tccgaagcac aaggtcgggg gttcaaatcc ctccgggcgc
      121 gccactccaa atccttgtgc cggcactttg ggttcaagca ttggaatttt ggccggttcc
      181 tagctatgcg ttgtcatccg aagtcgcagc gagcggcttt ctaaccatat taatgctggc
      241 cttggtagcg tatgcgtgtt cgtacatcaa tgctgcgacg cgacctccct ctccgttttc
      301 taatgcctgg acgatcaagt gatgctgatg atgtgcagag tagagatagt cgtacattcg
      361 cgggaggttc acattgtcga aggcgattgc gtacgccgcc gcgaacggaa ttcgatcatt
      421 gcgttcgatc gtgtctgcga tcaccttact tccagcgccc tcgatgatga gtgaatggaa
      481 ttgcttattc atctcgccat accgggcctc gtcggactcg aggagatgcc gcttggcaaa
      541 aatctcatcc ccgtccctca agcactcgtg cagtgactgt atgagccggc gcggcggtcc
      601 ttgctcggcg agcatccgag ccgctagtcc ttcgagaacg ccacgaacgt caatcgcgtc
      661 cataatctct ctgggtgtga acgcgcgcac cacgaatcct cgcgtgtcga gctgggttag
      721 cattccctcc tgggctagaa tcggcagggc ttcgcggacg ggagtcctgg aaactccaag
      781 cttagcagca agttctactt ctcttacccg ctgacggggt gtgagctcgc catgcaagat
      841 catctcgcga attcttacga ttactcgtga gtttttaccg gccattttgc acgcgattcc
      901 gaatgtaccc tgctgaggga cgggactatc gagaaggtta aaaatccttc cgtccaccct
      961 cttcgtgaaa gatagcagac actgacgata gggcggatgg tatcaactcc gcaccgcgca
     1021 agcaatcgcg ctccgtgttt ttcacatgaa gctgtttttc cgatcagtaa cggtgaagcc
     1081 acggaccgat cctcggggct tggatggtgc ggaagtcgcg cagccggcag aatttctgat
     1141 tgattcaaac ggcaagattc cgtgggtgaa tttaaccggc agctaggcgg gcggtaggac
     1201 gatacaaatc accccaaaat cgtgccttca ccacacctca ccgtggacga tttttcttta
     1261 caccaacaag tacttgcatc atcccacggc cttaattcaa aatttacctt gtatcctcgg
     1321 actacgccca ttgccccagc agtgtcctcg ggaggggtcc tattcgcggg accttagcaa
     1381 ttgcacctgg gaatacaaac ggcagtcatt gcggtgcgga acgccccacc atgaacacct
     1441 aggcagcacg tccctattcg cgctcggaac ggagttgcat ggcgcgcgtg agtcctgctt
     1501 cgcgtagggc ctgcgatagc cgcgcgcagt cgagatgaat cgaatgctct ttcctcagta
     1561 ttttctggaa tgaacgtgcg tcaaggtgag aatactgctg ttgatagagc agcagaactt
     1621 tttccaagtc cgaaagaggt accggcagtc cacctggttt tcgcgtcgct cgcacccaga
     1681 agccgtcgta gcctcgcctc tgatagagcc ctcgcaaacg ccacatcgca gaatgtgaca
     1741 ttccaagaat gtctgcggcc tgatcccagg taatcttctt tgcgatcgcc cttagaacca
     1801 cctcgcgcat tctcatgttt ggcccccgct agcatgcgat ttggtcactg caggatggat
     1861 tcgcatgtgc gttcgccgag tgacgctcgc ccccaaccac agggacggtg attaaaaaac
     1921 cttggcgagg ccgctgaccc gccggcatcg ccaaggttcc tgaagtgggt taaaaatcaa
     1981 gctttagcgc aaactgaatc tgccgtggcg tcacgacggg cgactgcgaa aacgtggttt
     2041 gcccggttgt accgcctggt gcattgattt cgccgagtat gcccgcccca gtccctccga
     2101 cgttgaaatg attgaaacag ttaaagcatt cggtacggaa ctgtaggttc agtttctccg
     2161 tgatcttggt gttcttggtc acggaaagat ccgcgctgaa cgttccggga ccgggtagac
     2221 tgtctcgctt gacgttaccc aggaagcccg gagcctgcgc ctcatagcac gcgggatcga
     2281 accattggat ggtcgtcgcg ttgatcctct tcttgaggag gtggttggga tggcatcccg
     2341 gtgcgaacgt gtaatttgct tgtgaacccc attgggtccc caagaacgcc ggatcggttg
     2401 aggtgagggt gttgttgtag acgttcagcg gcaagccggt gtgaatcccg acgatcgctg
     2461 agatctgcca tccctctact aaccgatttc ccttgaaagg tagggagtag agtccattgg
     2521 cactgatgtt gtggcggatg tcgaaggtgc agttcccgta ggcatacctt tcgttgtagc
     2581 gatcgacgac cagttgggcg aattcctcga gggatgaggc aaaagatcca tcgtcaacgc
     2641 agcgggacca tgtgtagttg aactggccgg caatgttgcg cgcgaactgg cggttgaggc
     2701 tcacttgtag ggagttgtag gccgaggccc ctgtgttgta cgcctgtatc agcgatccga
     2761 atgctccgtt tattcgaggg ccagtttgag cgaagggtgc aggaggtggg aattgcacgc
     2821 cgctcccata acaactggtc tgggttggaa agtggcaggc gttcgatacg gtaaaggtgg
     2881 cgtccgaagc ggttgggtag gactttggaa tctgtggcaa ggctttgcag tctgggaacg
     2941 tgagacactt cggaggattg atgtcaccct gtgtccacag gtggcgcgac acggagccca
     3001 catatccgac ggacaaaact gtggagcgcc agagttcacg ctgcaggttt aagttgtacg
     3061 tgatctggta cggagactcg aacagagggc cgcccctggg ttgataggtc acgccggcaa
     3121 attcgcttac ggcacccgga gggttgcacc ccgttgcaca tagattcggg aaactgggga
     3181 aaacaagaaa cgaggagcca gagggagcgg tgttgataaa gttcgattcc cagagccgac
     3241 ccgacgtggg atcagagaag ataccgacgc ctccgcggat cgaggtcttg tgatcccgga
     3301 agggatcaaa ggcaaatccg aagcggggct cgaggttccc ggcgttgaca ttgttcgcga
     3361 agacgtgcct gacgggcgta aagacaggag cgccacccgg aggcgcgacg gggccaacgg
     3421 gcggaagaaa cgaccccacg agcgtggtca tcggctggtg tgggaaagcc cagcccacgg
     3481 gattggtatc gtaatcgtag cggaagccga ggttcaacgt aagccgcgag cttactttcc
     3541 agtcatcctg aatgtacgga gccaccatga tttcccggaa gtatcgggtc gcgttgttca
     3601 aagctggaac cgttggcaca gcgaacgcca ggaaaggtgt tccactcagc caaaattggg
     3661 catcatagac cggcgcgggt actccgtaag aaaggaagaa gccgccgttt gagtaggctg
     3721 tctggagatt ctgggtttgg actcgggtga ctaccgcacc gatcttcagg ctatgagcac
     3781 catgagtcca agtgatgtcg tcgccgccgc tgaacttatt ttgaacgatc tcatcgggcc
     3841 gattcggatc cgggccgatg gggaagatga cctgctggaa gccgccattc tggccgtcct
     3901 gccgcggcaa ttcgccgggc tgggtatcca cgaagtgcag aggatcgctc gtaatagccg
     3961 gccctcccag tgacgaactg aacgcattcg ccgccgacag ttgggcattg gtaagggcgg
     4021 gctgagcctg tgaattctcg ttgtttcgta cgtacccgaa gtgaatcgag ttcaccaacg
     4081 tggcggaggc gacgtgcctt tccgtgattg tcaggaactg gtttcgcgtg tgatccgttt
     4141 cagggaagat ttggtatgga tcgcgcgggt cgcctacgtt tgcgttatcg aaggtaaacc
     4201 gggcaaagag ggaatcattc gacccaagcg tgtagtccac acgccccatg tagtagtttt
     4261 cctcgatggt caggggcggc gctgccgcag tctcgaaata tcctcccaga tcgcagccta
     4321 tcgtaacgca gtttccggga aggttcgtgt tgaccgcccc tatcgtcggc gtaatcaggt
     4381 ggcacccttt gcaaaggctg taaagcgccg cgatttgctc cgtcgtggta ttcgcacctg
     4441 gaacggctcc aatcggaatt cctgcctgtg tgctgttggc tgaggtcgat ggagtgcagg
     4501 tccctggtgt cggcgtaacg agactgcatg gaagcattcc ctgcacggta taaggctcgg
     4561 gcagaaagat gtcggtcgtc gttgcaagac tttgccggag gccctcgtaa tttccgaaga
     4621 agaacaattt gttcttcttg atcggaccgc ccagatcacc gccaaattga ttgcgcctga
     4681 atggtggggg gccggtaacc ggatcgacta tgtcgcgagc gtccagaacg ttattgcgaa
     4741 agaattcata cgcaccgcca tgaagatcat tggttccaga gcgcgatgtc gcgttgatga
     4801 cggccccgtt gccggcaaac tgcgcggtat aggtattcgt gagcacttgg aattcgccaa
     4861 tggcttcgac gccgagagac gtcgcggcgt agccggaccc ggttccgtgc tcccagaagt
     4921 cgcggatatc cgtgttatcc agcaagaaca tttgcccggt gggtcgtgac ccggcgaccg
     4981 aatagttgtc ctgcattcca tagagccgac ccgttaccgc attgagcgct gctcccaccg
     5041 tggacacacc gggggccaga gtaatgagct gctcgaaatt gcggccattc aacggaagat
     5101 cgcgcatttg ctgcggactt atgagggtcg aaacttcaga cgtttccgtt tctacgcgcg
     5161 aaacatcact ctcgacgttg acggtctgcg ttacttctcc gaccggaagt gagaaatcga
     5221 cgacaagcgt tccgccgaca ctgagtgtga cgcctttgtg cacgactgtc tggaatccag
     5281 aaagcgaggc ctcgacactg tacgtgccaa ccggcagttg ggcgatcgtg tagcgcccct
     5341 gggcatccgt ggtagtcgac tgcgagatgt tcgtgccaac gtttgttacc tgaatatggg
     5401 cccccgctag cgcaccaccg gaaggatccg tagccgtccc aaaaattgtg gcggtttgcg
     5461 cctgcccttg cacccgcccc gcgctaaaca gaaacacgag tgccgccaga aacacgcccg
     5521 tccagacaca cttagtcgta ttggagcccg ttgacatttg cccaccctcc tcgatgccac
     5581 ggaatatttg aagtcaggtg tgtcgcaacc ccagcgccac actccctgtg cgaggggtga
     5641 tactaccaaa gtaatgtctg tgtcaagata aatatgtata cattaaggct aggctttgga
     5701 cgtgacgact cgagcttcat ttcgcctgcc gcctcataac atcttcgcta tctgcgacat
     5761 cagatggcct cagaggcgcg gctgcctttt cctcgcgacg cgaattatgg ctacaggagt
     5821 gtgcaagagc gatacaattg gattgacagg acctcgctaa tcccttatct tcctgccgac
     5881 tttatcgagg agagatggtg agctacaaga gcctggaaga ccttttgcaa tccgtcggta
     5941 atccggtaac gctgctgcga aattcgcaga ccggcccata cgtttatcct gtagttcccc
     6001 cggaatattc caactggcga gacgagcagc gcgcctggca acagacctgc gttttattca
     6061 atcagtcgta tcacatggag gacatgtaca tctcaggccc cgatgcgacc aggctccttt
     6121 cagcccttgg catcaacagt ttcaagggtt tcgagccggg cagggcgaag caattcgtcc
     6181 cctgcaacta cgatggctac gtgatcggcg acgtgatcct cttctacctg gaaaagaata
     6241 tgttcaatct cgtcggccgt cccgcagttc acaattgggt gcaatacaac gcggaattcg
     6301 acaagtacaa tgtgacattc gatcgtgatc cgcgagccgt cgcacagaag ggtccggtcg
     6361 ttcggaaggc ataccgctat cagttacagg gcccgaatgc aatgaagctg acggaaaagg
     6421 cgatggggaa acccgcgcca gaactcaaat ttttccacct gacaacgatc cccatcgcgg
     6481 gacgaacggt gcacgcgctc cggcacggta tggtcggaca accagggtgg gaattgtttg
     6541 gaccctggga agacggcgaa gcggtgaaag ccgcgattat cgaggctgga aaagaatttg
     6601 gcctgcggct ggttggcgcg agagcgtatt cgtcgaatac actcgaatca ggatggattc
     6661 cctctccgat acccgcgatt tacagcggcg aaaaaatgaa gccgtaccgg caatggctgt
     6721 ccgcaaacag ttacgaagcc atcgcctcgc tgggcggcag tttctactcg gacaatattg
     6781 aagattatta tctgaatccc tgggatctgg gatacggtcc catcctgaaa ttcgaccacg
     6841 aattcatcgg gcgagaagcc ctcgagaaaa tggccaagca gccccaacgg aaaaaagtaa
     6901 cgctcgcgct ggatagcgac gatgtgaccc gcgccatcca caccatgttt caaaagaaag
     6961 accgcgccaa gtatttcgat tttccatccg ccgtttacgc cacgctgccg tacgacaagg
     7021 ttatgagcga cggcaaaata gcgggaatct caacgtggtg cggctacagc tcgaatgaag
     7081 gcaagatgct tacgcttgcg attctcaagg aagaatatgc ggagcccggc atggagctca
     7141 cgttcgtctg gggagaagaa ggcggaggct catcgaagcc aaccgtcgaa cctcatgtgc
     7201 agacgaaaat tcgcgcgatc gtgagcccgg tgccttactc ggaagttgcg cgcacgcaat
     7261 acgccgaggg ttggcgcacc gcgcaaaagg tgagctgaga gcgagattcc gcgtcaggcc
     7321 acgcaatcta gcgaatcaga tatcgtcgat ataagaggtt cctatggcat cctccgcacc
     7381 tcataatccg ccatttcgcg ctgaccacgt cggcagtctg ctgcgtccca ccgagctcaa
     7441 aaatgcccgc atggactgcg ccgcagggaa aatctcggct gacgatctgc gttccctcga
     7501 ggatgccgct atccgaaagg tcgtcgctcg ccaggaggag atcgggctgc aatcaatcac
     7561 cgacggcgag tttcgtcgcg aggatttcta taccgatttc tacgtgcgag gactcggcgg
     7621 cgtggaagtg cgcatggaaa gcaccgccgc ctacttcgtc gatcatgagg gacgcaagat
     7681 tcctgtcccc tggacccaag tcgtctcgcg catgcagtgg cgcaagccta tctatgcgga
     7741 tcatttccgc tttcttcggt ccgtcacgac gcaaacccca aaggtaaccg ttccttcgcc
     7801 catcattttg cacttcacct gcgggagcgc cgccattcgc gcgaatgcct atcctgatat
     7861 ggacctcttt tggacagacg tcattgaggc ataccagaaa gaaatgaact cgctgtacga
     7921 agcgggctgc cgataccttc aagttgacga tcccccgatg gcgacgctgt gtggcgaaaa
     7981 tttccaggcg atgatgaagt cccgcggcga tgatcccgag catgcgatga aagtgctgta
     8041 tcccgaaatg attaaccgcg cctttgcgaa tcgtccgcct tcgcttcacc tggcgatgca
     8101 cttatgccgc ggaaacaatc agagcggttg gttcggcgag ggaggatacg atccgatcgc
     8161 cgaagtccta ttcaacaaga tcaacgtgga ttcatatttc ctggaatacg aaaccgaccg
     8221 cgcaggcacc ttcgagccgt tgcgcttcgt gccaaaaaac aagaccatcg tgctcggcct
     8281 ggtttcgagc aagcttccta atctggagtc caaggatttg ctgaagcgtc gcatcgagga
     8341 agcctcgaag tacgtcgata tcgaccaact ctcgctcagt ccgcagtgcg gattcgcaag
     8401 ctccactgaa ggcaaccgcc tcaccgaaga ggagcagttc gcaaagttgc gattggtcgt
     8461 cgaggttgca aacgaggtgt ggggccacaa aaaaggttcg tcgtccagcc atactgcttc
     8521 cgcaaacact tgatcttcag catcccgcct cgcgccgcgc gctataataa tatttggcct
     8581 cataggtggt gaaaaatgaa aatgaatcgt agaaagttat tatctcttct tggagtttcc
     8641 cccgtaatcc tcgccggcac ggcatccgct aaagatcgca gtaaaaaaac gccgtcgtca
     8701 gcctcatcgg aatcaaagat tcaaggcctc aatcccaggg gcataccgcc ctccatacaa
     8761 ctcattccca tggctcctcg tccggccagc ctcgatggca agacggtcta tctggtttcg
     8821 gatggattcc ctggcgcgga tcactttctc aatcagatcg cgatttggtt caagaaaaac
     8881 atgccaagtg tgatgacgca atatcgcctg aaaaagggtg gattcgccga tgatgacccg
     8941 gccctctggg ctgaaattaa agccaagggc agcgcggtca ttatggcgat tgggcattga
     9001 agcacctgca ccccagcgac cgtcggtcac tgcatgacac tcgaaaagat gggcatcccg
     9061 accgctccca tggtcacaat cgctttcaaa gacctcgcaa aaacgaatgc cgcggggcga
     9121 ggaatgcctg acgaacgcat ctgctttact ccgcatccgg tttggggcaa gacggatgca
     9181 cagatgtatg cctacctgga agggaacgat cccgtcacgg gcaaaccgct gatgaaggaa
     9241 gttatcggag cgctcaccaa cccgctcacg gctgacgaaa aaaagaccgg caccgtcacc
     9301 ccttcggtcg gtccacccat gtttgtcgac acacccgata accttcagaa atattacaaa
     9361 gacaacggca tgaccgacta catgccgatc atcattccaa cgcaggaaag agtagacgca
     9421 atgctgaaag gcacgagtca cagtcccgat gaagtgatcg gtgaattgac tccggcgcgc
     9481 ggagctttcc cagcgtggaa attcactgtg aaacacgtgg ccatcagcgc ggttatgtca
     9541 ggagctgacc cagattatct cccaactatt ttggcgctcg cggcgactgg gcttccatcg
     9601 ctgtccagtt ccacgaactc tttcgccacg gccgccgtta tcaacggtcc cattcgcgac
     9661 aagctcaaca tgaattacgg aatcggcgcc atgggcccgt ttaacgattt caacgcctcg
     9721 gttggccgcg cctggaccct tttctccaaa aattgcggca attgcggcca ggcgggcgaa
     9781 acctacatgg gcacccaggg caatgccgtc aatttcggca acatcattat cgtcgaaaac
     9841 gaaaaagaca gtccgtggaa accatttcac gttcaaaagg gtttcaaacc ggaagacaat
     9901 gtcgtcagcg tctttttcgg ctacggtatc gcgcagggac aaggcggcaa gggcagcgcc
     9961 atcaaacctg tccccgcatt cgaccaaggt ctcgtgtaca acttcatacc gctaacctcg
    10021 atcttcggcg cgcttgcggt tctcgacccc ttggtggcta aggccctcgt ggatcgcggc
    10081 tatgacacga aggaaaaact caccgcctgg ctgcagaaaa actctaccct taccgtaggt
    10141 gatgccaaga ccatgttatt caccggaggc gccgccttgc gcgggcccca cgcggccgaa
    10201 ctgaaggacg atgacctaat cccccactgg ccgagtccgt ccatgatcaa cgtcgtggtg
    10261 gtaggcggac aaaccaatcc ctactatcaa gtcgccaaca tgagctacgc aagaagtgtg
    10321 gccatcgaca agtggatgta gagcagcgtc gaagtcccat gttcctcggc gcccggtttc
    10381 acccgaagag ggctgtcctc gggcggccgg ccgcatcgtg gggacacgcc aacctttggg
    10441 cggcatgaac tccactctta ataggaagta cgactgttat atcgcgccct cgcccggaag
    10501 cggtcattga gaccaagcgc cacaatgccc aacaggcctt cgccaaatag gagaatcgtg
    10561 ccaggctccg gaaccggagc ctgtcctgcg ctgggagtgc taaaggtgag gtccgtgaga
    10621 atcacggcgg agttcgagac ggtgaaagac gttatctcag gactgtcgac tgaaataccg
    10681 cggaaagctc ctgcatccac gccgccgggg gtgctaatcg gagccagtag attgatatca
    10741 aaagcaccta acgaagtact gccatcgtag gcgtcgatcg tcatgtccga gcacactgcg
    10801 caaggagcat aattcatgaa tgcgcccacg gcactaacag gagtagcaaa agtgaaggtc
    10861 atcgtgcccg ggaacccacc cagtgctacg tagtgaaggc tagaatccca gactccgttc
    10921 gaatcggttc cagatagcag aaaataattc ccactgccga tggagctctc ggtggtggtg
    10981 ctgctccacg tcacgcccga tcccagatcc accggaccag aagtaaatgt gatgcctggc
    11041 ccgaaggcgt cgaagtcaat aaccgaggga gaagtgattg aaactgcgga ggtcaacaag
    11101 gtatccgcct gcgccgctat cgttaaaaat aagctggcgc aaaacagccc aacgaccgcg
    11161 agcacacacc cgatttttcg gcaatccgtc atcggctctc tccggaatca gttcgagaag
    11221 ataaggctcc gtccgcactt ttcggcccaa accccggcgt ttgcacgcaa cagcttacct
    11281 gacaactcct cggtgatatc tgcaaatgaa ttaccataaa aggaattaac agcaactgcg
    11341 ggggccatta ttttgcttcc gaggtaagct cgctattctt gatgaagcac gggttcgggc
    11401 cgccctcggc ggctcattcc caaccctcgg gcaaggcttc catgccccga cctgacgctc
    11461 agtgggctgg cgccgtcttc ggcaacggag gcggaatctt catctgcttc agcttcagct
    11521 ggtactccgg atacatcgtt tctgcgccgc ctcgcgttgc ctcaaaggga attcggtatc
    11581 tccgcgcgaa ctcctcgaga aatttattcg tccccggtaa gtgactcggc acgacgcctg
    11641 gtggccggtc cacctcgtcc acgggctcgc acggccatgg cgccatctcc atttgcgggc
    11701 tgtaaatgaa atctgacgtt cgaatgagag gctctgtaag gtaaatggga tcgtaggtta
    11761 tcgccgcgac agtaagatag tcgccgtgct tcatgatatg ggcgctagcc gtggctgcgt
    11821 cgctgcgggg cacttcgttg agcccgagcc aattttcttt caggtgcgtg gtttcgatca
    11881 ccagcgtatc gccgtcccaa tgcccggtcg aaaatccctc ccaggtatgt cgcgcataat
    11941 ccggcggatg cgggcgtcca tccatccaaa tcgtttgctc cgctccgtag gctgaaagat
    12001 gggtgcgata cgcgatcact ttctgcgatg ctttgtccac ttccttccag atgctcaagg
    12061 ccgagggacc gcgccatccg tagtctgccc cgtgctccct gcactgattt tctggcaact
    12121 ccacaagtgt ggcgtcccag ctatcggccc tcagtctcgc ggcagcgttg atgggaagtc
    12181 caagatagtc cccgatctct gccccagcca gccgctcagg tatgtcctcg taaaacaggg
    12241 gtccccacat tcccgttgga tccacctggc caaaagcagc tccggcaaat acaccggttg
    12301 aaagcaacaa cagcaatatt ggcaaaatgg caatccttgc tcggatgatg gcggtcattt
    12361 ctgctccaga gaagatcctt ggcctttaaa attccctttt acctatcgcg cccgaacata
    12421 gtaactttct ttgtggctgt caatgaatgt atgcaacgat tctccgcggc acgctgaatc
    12481 gccttgatgt atcgggatat cggctgcggc tatcggattt gtatgcactt catgttgaca
    12541 cccaaaggaa acttgtagta actagcgcag cgcgcaaggc cataaaatag gaccatctcg
    12601 ccgtgaatcc aatgaattcc ttggaacccg tcacgctggt tcgcccatct aggagggcgc
    12661 tatgaactat cccgctactg gatatcacca catcaccgct tgtgccggcg gtgcgcaaga
    12721 agacgtgaat ttctttaccg gaattgtcgg gcagcgtctg gccaagcaga caattctgat
    12781 ggacggccga ttcccccatt atcatttgta ctactcgaat gcgaatatcg aacccggctc
    12841 cattatgacc acatttccct acaaccgcct tatggggcgt cggggatcgg gccaggtgca
    12901 atcgattacc tataccgtat ccaaaggcgc gctccctttt tggcaggaac acttcaagcg
    12961 ccacaatgtc gatcacggcc ctatcgagga gcgctttggc caacgtttca tccgtttcac
    13021 ccatcccgtc ggcatgactt tcgaggtttt ggaagacaaa gacgaccaaa ggattgggtg
    13081 gagcactcca gaaatcgctc agggcgaatc tgtacgcgga ttttacggca ctgtgctatc
    13141 cgtccgcgat gtgactgaac aggagaaatt cctcaccgaa gctctcggtt tccgcaaaac
    13201 aggggtggat ggtaagtacc atcgtttcga gattggcagc ggcggcgcga ataaaactct
    13261 aatcctcttg catgagcctg atcgggctcc gggcagttgg accttcggcg ccggcacggt
    13321 ccatcacatt gccctgtggg tcgccgacga tgaagcgatg gccgcgcaga aagccattta
    13381 cgaagagctt ggctacacgg acgcatctga aatcaaagac cgctattatt ttcactcgat
    13441 gtacgttcgt tcgcccggcg gcattttggt cgagtgctgc tgcaatgttc ccggaggctt
    13501 tgaaaaagat gaaccggcgg cgacgctggg aaagaagctt catcttcccc catggttcga
    13561 agatcgccgc gacgagattc tcaagacact cgaaccgatt gtcgctcctg aaacggcaac
    13621 gtctcgcgcc tgaactcccg ggcgaagatc ggaaaaaact cgactccatg aaacgaagcg
    13681 agaaccgaat tctcaccacg cacgtcggca gcatcgtccg tccgcaacag cttcgcgatc
    13741 tcgccgacga agccgcgaaa tcccccgaaa atcagaagcg ctacgaggat ttcttgcgcg
    13801 attccgttgc cgacgtggtg aagttgcagg ccaaggctgg cattgacata gtcaatgacg
    13861 gcgaattcgg aaaatccagt tggtcgaatt acattctcga acggatcacg ggattcgaac
    13921 atcgacccga tcaactgcac ccggtctatt ggctgggtcg cgatcgtgac cgcttcccgg
    13981 aggtgatggc tgcggaattt ccatggatga cgaaaggggt ggcgaccgaa gcgtgcatcg
    14041 ggccgatcaa atatcgcgac cacgccaata tcgatcgcga tattcgcaac ctgaaaggcg
    14101 cattaaagtc cgtcaacgtt gaagaagcgt ttcttcctgt cgtcgcgccg gcaagctccg
    14161 cgtacgacgg cgtcaacgaa tactatccca cggaaaaaga gtacgtgtac gccatcgccg
    14221 acgccttgcg tgaagaatac cgcgccattt acgaggccgg cctgattgtc cagatagacg
    14281 acgctgtgct agccaacatg tacgatcact acgtcgagca aagtcccgaa aagtatcgcc
    14341 agtgggccgg cctccgcatt gaggccctca atcatgctct gcagggcatt cctgaagatc
    14401 gagttcgcta ccatgtctgc ttcggcagtt ggcacgttcc gcaccttgcg gacgcgcctc
    14461 tcgaggaaat cgtcgggttc atcctgagcg ttcgtgcagg cgcgtattcg atcgaagcgg
    14521 cgaacccgcg gcatgagcac gaatggcgcg tttgggaaga aaagaagttg ccggctggaa
    14581 aaattttaat tcccggtgtt gtcactcacc atacgatgac ggtcgagcat ccgcgcctcg
    14641 ttgccgatcg catcctgcga ttcgccagga tcgttgggcg cgaaaatgtt atcgccggca
    14701 cggattgtgg attcgcgcag gcgcaattca ttcagcgtgc ggccccatcc gtcatgtggg
    14761 cgaaattcga atcgctcgtg gagggcgcgc gcatagccag cgccgagctt tggcagaaag
    14821 gaaagcgcgc ccaaccttcg taaccgtgcg acgcgaagcc gcggaaattt tggcggtgac
    14881 gaaaccgaaa gtgcgagcct aggtcctttc ttcataagtt tagttgaaat caccacaagc
    14941 attgccgttg gtgcggcatg aatgggggaa cgccattgaa aaccttagct caaagagttg
    15001 cgtggctggt tgcattgatg ggaattctgg cgttcgcaca gccggcccga gctgcagcgg
    15061 ctcagtgcaa tcgcgcctgc ctgaagaata cggtggacaa atatctcgcc gcgctcgtcg
    15121 cccacgatcc ctcgcaagtc tcattggctc caaacgtcaa attcgtggaa aacacagtcc
    15181 cgatgcatcc gggcgatggc ctctggaaaa ccgcatccgc ggcaccaacc actttcaaga
    15241 tttacgtgcc ggatcctgtt tcggaagagg ttggcttcct gggcgtcatg caggaaaagg
    15301 gcaagcccat cgaactggcg ctgcggctaa agatccgcaa cggcaagatc gtcgaggcgg
    15361 agcacctgat agcacgcaat ttgatggtaa gaaatctggc taacctgaag acgcctcgcc
    15421 ccggcctgct ggcgacagtg cctcccgacc agcgcgtgcc gcgagaccag atgctgaaaa
    15481 tcggagcctc ctactacgag gcgctcgtca ctgctgatgc caacaatgcg ccgttcgccg
    15541 atgattgcgt gcgccgcgag aatgggatgc agacgaccgg caatcccccg cccgcaaagc
    15601 ccggacgcgg aactatcggc gctatgggat gtgcgcgtca gttgcgtacg cacacgttcg
    15661 actacatcaa gcgcatcgag ccgcgccgcg tggaaattgc ggatcctgaa accggtctcg
    15721 tgtttggcct ctctcaattc cgccagccga tgaaggaaaa gacggtgaaa attgttggcg
    15781 tccccaatgt cgaccatgtg gatttgaaca tcaagccctt cgatcttccc gccgcgcaca
    15841 ttttcaagat tcgcaacggc aagattcacg aaattgaagc catgggtttc acgatgccgt
    15901 acaactcgaa aactggctgg gaataagcct gcgaaacgac ctgaagcgaa tctgttcgca
    15961 tgcctgcgat ttccgtgtgt tcgtccgtat cgtatcctct ggtcacgcta atcgggcgct
    16021 tggatctcgt ttgctgaagc gcgtgtataa tgcagccgcc cagggagggg ctgaaaaatg
    16081 cgcatgttac tcaagatttc gatacccgtg gaaccaggca atgccgcggc tcgggctggc
    16141 agcttggaga gcaccatcaa gagcattctg gacgagctga agcccgaagc agcctacttc
    16201 gccgaagacg gtggcgaacg taccggatac atcttcttcg acatgaagga atcgtcgcag
    16261 ttgccggcca tcgccgagcc atggtttctg gctttcaatg cgaagataac cgtccggccg
    16321 gccatgactc cgcaagacct cgccgccgga gcttccggca tggagcgagc tgcgaagcgc
    16381 tacggcaggg ccgcgactgc ctaagggaac agcgaaaaaa tgttagcgcg gtaagcgcat
    16441 cgggatttgt gtgtctatcc gcttcccctt cctgatttta tcaatcgcta aagtgcctgc
    16501 tccggtgtgc cagcccctaa ccagacgccc cgaggaatcg cccgattgac atggacgtgg
    16561 ggcctaggca tagtcttttt ggggtcatct ctaccgatgg ttttcagaag ccgtcgggtt
    16621 tcggacgggc tttgggcgaa gcctttctgg ttggccgtcg tttgcatgat ggtttgcttg
    16681 acggcgacat acggccagaa cacggacgaa acccctcaac aaacgaatca aaagatccag
    16741 cagctcgccg cgcaagctca ggctcacccg acgaatacgc cgatcgggag cggcgatctg
    16801 ctgcacgtcg acgtctttga cgtgccggag ctttctcgcg atgttcgagt gagcgacacc
    16861 ggggagatca gctacccgct cgtgcccggc aaaattaagg tggccggaaa gactccgttt
    16921 caacttgaga cggagatgga gcggctgcta atcgaaaatg gcctcgtttc gcacccacaa
    16981 gtctccgtat ttgtcagaga gcagactagc cagccggtat cagtggtggg cgcggtaaac
    17041 cacaccatgg tttatcaggt ggtccggcct acgacgctgc tggaggtttt gtcggcggcc
    17101 gggggcatct cggatacggc cggggatgtc gtgattgtca cgcgccccaa caatcccgat
    17161 gttgcgaagg ttgagcccgc gagcgccaca accaagtcgc agccggacga gcaaaaaata
    17221 attgtgcgca tccgcgattt gctcgagtct ggagactccg tctacaacat tccgatcctc
    17281 ggcggcgaca cagtgacggt ccccgcggcc ggcatcgtct acgttctggg attcggtgtg
    17341 gcccagcctg gcggatatgt actgcagagc cacggcgagc aagtcacagt gctgaaggcg
    17401 ctggctctgg cccatggcct gacgccgttt gccaaatcca actccgccgt catcatgcgt
    17461 gagaaccccg ccacgcggca gcgagatttg atccccgttc atatcaagga gttggagaaa
    17521 aacaagacag ctgacatacc cttgaagtct aacgatattt tatatgtgcc cgacagcgcg
    17581 ggaaagaagg cgctggcgaa agggagcgag gcggcgctgg ggatcggtac gagtttggcc
    17641 gtttatcgcg tggcatatca ctagcgttgg actcggcata ggggaactgt aacaacccgc
    17701 tcaagagagt gcgatggaag acgcttccag atacgcgatt cgtaagccgg ccgagcagct
    17761 ggcggtcatc acgcccccaa ggaatttggc tgcatgggag cagatgccgc gagaggctca
    17821 tctgcttgat tacctcatta tcctgcgcaa gcaccagtgg ctgattctga cctttttgct
    17881 gaccgtggtg acggtggtca ccatcgctac cttcaagatg aagcccgttt atgaggcggc
    17941 cgcgcgcgtc gaggtcgata gggaagccca gaacgagctg ccgttccagc ccacgaattc
    18001 ctacgacgaa tatgcggaca tggatactta catcgaaacg caaaccaaga ttctgcagag
    18061 cgagactcta gccctgcaaa ccatcacgag catggatttg acgcagtatc cggagttcgg
    18121 cggtaatcca gcggcgggaa attttgggcg aggcggctcg gcgataaaac gccccgcaat
    18181 tttgggtgcg tttctgggaa gtctgagcgt tgttcgcgtg cccaacagcc gcctgatcga
    18241 ggtccgattc gcggctcaga atcctcagct cgcggcccag gttgttaatg cgcatctgca
    18301 aaactatatc gagcagaatt ttcgaagcaa gtacgacgcg accacgcagg cctccacttg
    18361 gctttcctcg gagctcgaag agctgcgtat aagagtcgag aagtccgagg acgcccgtct
    18421 cgcctacgaa cgccaaaatc aaatatggca gattgacgaa aaacaagaca tcaccaccca
    18481 gaagctaggc gatctcagca gggcggcgac agcggcgcag atggatgtcg ctcagaagga
    18541 agccctgtac cgaatggcaa cctcgggaaa cgtggatgcg ctgccggccg ctctgagcaa
    18601 cggtgtagtg caggatctgc tacggaaaaa gtcccaactc gacgaggatt atgcccaggc
    18661 cataaatcaa tacggcccca actttcccaa ggttttgcgc ctggccgcgc aacagaagca
    18721 agtcgatgcc gatcttgcca atgcccgcaa gacgatggtg gaaagcgtcg aagaagatta
    18781 caacaccgcc cgaagccacg cggccctctt gcaagacgcg ctcgacaagc agaaagcgga
    18841 cgccaacgac ctcgctgaaa agctcgtcca ataccatatt ctgcagcacg acgccgattc
    18901 aaataagcag ctttacgatg gacttctgca aaagttgaaa gaggccggaa tcacggccgg
    18961 attgcgatcc agcaacatcc ggatagtcga tccggccctc gtgcctacta ccccttcgcg
    19021 gcctcgaaag gccagaaata tcctgctggc attgatcgtg ggattggtgg ggggcattgg
    19081 cctggcgatc ttccgtgagt acttggataa tactgtgaag tctcccaccg acattgaaaa
    19141 tttgaccggt ttgccctctc tggcagtcgt tccattcttg cctgcattga atggccatca
    19201 cggcaggttg tcgcggattg cgcgtgaggc gaccgggctg gcggcctcaa gcccccatgt
    19261 ggaactgctt tcttacgttc aaccgaagtc ccaaatttca gaggcgtttc gagcgcttcg
    19321 tacctccctg ctgctgtccc aggccgagca ccctccccag gtgatcctcg tgaccagcgc
    19381 cctgcctcgc gaaggaaaga ccactgccgc tgtgaatctt gcggtaactc tcgcccaact
    19441 cggtgatcgt actctggtaa tggatgctga ccttcgtaaa cctggcattc gccgagcctt
    19501 gaatctcact tcaggaaggg acatcggcct gagttcctat ctcgccggcg tttcgtctct
    19561 ggatgaagta accacgtccc acccggccat aagcaatttg gcggctttga ccacgggtcc
    19621 ggtgccgccc agtccggcgg atctgctttc ttcccatcgc atgcgcgaga ctatcgcgga
    19681 tttgcgcagg cgttttaaat tcatcgtgat cgattcgccg ccgattttgg ccgccaccga
    19741 cgccgttatt atttcttcgc tgacggatgg cgtcttgctc gttgttcgca gcggcgcaac
    19801 gcctaaggaa gccttcaccc gttccaggga tttgctgggt gccgtgaaga gccggcttct
    19861 cggcgtagtg ctgaatgccg tggattccag cgctccagac tattactact cctatcgata
    19921 ctatccctat gcgtatggat atggttacag cgaagagggc ggcaagccgc cggaatcttt
    19981 cggtggtttg gatcacacgc cggaatctta aattcaggag gcgtcattgc gtcatctcat
    20041 aaccggaggg gctggcttta ttggatcgca tttgtccgag gcgctgcttg cccgaggcga
    20101 tgaagtattc atcgtcgatg atctctccac gggcagcgtc gagaacatcc ggcat
//
LOCUS       DATNDT010000486         4894 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2650192, whole genome shotgun sequence.
ACCESSION   DATNDT010000486 DATNDT010000000
VERSION     DATNDT010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4894
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2650192"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="VN788_15985"
     CDS             complement(<1..452)
                     /locus_tag="VN788_15985"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU22083.1"
                     /translation="MRLHRFAYLVFASVIGILFVPPVITHAQETATIHGTVTDPSGAA
                     VSGAEITSQPVDSRGMVRRVQAAADGSFSLPLVPGKYRLSVRHEAFAPVEQEFAFAAG
                     EMRTWDVRLQLERMSSSVVVSDTAEPTTAETAPDLVDVITKEQIDQRQA"
     regulatory      complement(515..724)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(808..1668)
                     /locus_tag="VN788_15990"
     CDS             complement(808..1668)
                     /locus_tag="VN788_15990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU22084.1"
                     /translation="MSGAAASNASVAASATAARQPGLRLKVEQASYAYAATDRSAPKF
                     ALGPVSFEAQQRELVAILGPNASGKSTLLTLIAGVNKPLSGRIEIDGVEVSQIDLRAR
                     AKQIALVQQESELLFPLRVWEYVLQGRYPYGRRLRFESAEDCLHAGNALAQVGADALR
                     DRWMDRLSGGEKQRVILARALAQRPSLLLLDEPTQHLDIGGKVELLRRLRRLADENRY
                     AVLVVTHELDLAAEFCDRIVLLHKGKCLRVGKPAEVYERALLEEVFEAPLEIEMSASG
                     RPRVVLRSGQ"
     gene            complement(1665..2681)
                     /locus_tag="VN788_15995"
     CDS             complement(1665..2681)
                     /locus_tag="VN788_15995"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HXU22085.1"
                     /translation="MRPLTPQRVAWICIGLAIVLFAAMVISLRVGAYPIGVRDIVMTL
                     VNGVLHHWDAIPAEFRDVIFEIRLPRILLGIVVGASLATAGAGFQALLRNPLADPYVL
                     GISSGAALGAIIALIVAPHTPGAIQVAAFTGAGVTTAAVYFLGRRGGQLDSATLLLAG
                     IVTASFLAAVIMFMMTTLSGRDLRGMAFWLMGDLASRPTVDSRWLFLVLVLGVGSIYA
                     TASDLNLILTGEQEARHLGVNVNRVKIVVYVAASILTGLAVSVSGAIGYVGLLVPHLM
                     RMMFGTDYRLLIPTSALGGAILIVLADALARIVVAPTELPVGAMTAIAGAPVFIYLMR
                     RRVR"
     gene            complement(2669..3676)
                     /locus_tag="VN788_16000"
     CDS             complement(2669..3676)
                     /locus_tag="VN788_16000"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HXU22086.1"
                     /translation="MRRERVWITMIAVAFVTSSAPRMIAAQTDRSRMVTDEVGREVKI
                     PAKVNRVVTLAPNLTETIYALGLEDRLVGDTNVCDTPAAAKLKPHVGDPQNPSLEAIV
                     ALHPDLVLATTSINRVETADALKNLGIAVYTTDPETVRGMLDSVQRMADAIGAPKQGT
                     DLVDELQQRLDAVRARLSERPMVHVLFVVWLEPLQTIGQNTFIADALRWAGAESVLIS
                     KQKWPHLSIEEVVRLQPDYIIFTNDHGGPAAAELADLRARPAWRDLDAVEQGHVVDVS
                     EEAIRPSPGLVDMIEHLARQLHPEAFSKIRTMPNGNKFQTSSASRAATRIAERCPECG
                     L"
     regulatory      complement(3728..3884)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01482"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The adenosylcobalamin (AdoCbl) riboswitch is found
                     in the 5'-untranslated regions of vitamin B12 (Cobalamin)
                     related genes in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF01482"
     gene            complement(3929..4759)
                     /locus_tag="VN788_16005"
     CDS             complement(3929..4759)
                     /locus_tag="VN788_16005"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HXU22087.1"
                     /translation="MPQTSRGVEVLGTLALVAPKLEDFQFVKVRVEGDVGHLTLDRPD
                     HNLLNERMLSELAGGINSLGDRNNTKLIILDSAAKAFCGGIEIGEYTQRRIFQLLDAF
                     HGAFTAMLDTSKPLLVVVNGSAFGGGAELAALGDLVIATPKAKFAQPEIKLGVFPPLA
                     AAVLPYLLGPKQALELVLTGETMSAERARELGLVNWLVPEDGLQKKIDEVTAKVTAQS
                     APVLTMAKKAIMGSLGLPLRDGVRNSMKVFLNELAELEDSQEGLRALVEKRAPKWKNR
                     "
BASE COUNT          973 a         1605 c         1338 g          978 t
ORIGIN      
        1 gcttgccgct gatcgatttg ctctttggtg atcacgtcca caaggtccgg cgcggtttcg
       61 gcggttgtcg gctccgccgt gtcgctcacc accacacttg aggacattct ttcaagctgc
      121 aatcgcacat cccaggtgcg catttcaccg gccgcgaacg caaattcctg ttctacgggc
      181 gcaaaggcct cgtgccgtac gctcagtcga tatttcccag gcacaagcgg gagcgaaaag
      241 cttccgtcag ccgcggcctg cacgcgacga accatgccgc gcgaatcaac cggctgcgag
      301 gtgatttccg cgccggaaac ggccgcgcca cttgggtcgg ttaccgtgcc gtgaatagta
      361 gctgtttctt gtgcatgagt aattaccggc gggacaaaaa gtattccgat gacgcttgca
      421 aaaacgaggt acgcaaagcg gtgcagtctc atttttcctc ctctccctct cgcgcgggag
      481 ctgaaggttg tccggaagcg cgcgcttggc ctgcacttcc ggattgagac tccgccggtc
      541 tcctggcttg gcgaattgtc ccgatacatc cgggacctca gcgcggcctt cccatcccgc
      601 ctgcgaatcg cgatcgattc gccggccggg ccagtggcac gcgccgaaaa acttccgcct
      661 tacagtcgcg cggcggcgac ggtttttcac cgtcttcccg tgcgcggagt ccccttgtga
      721 ttatttgcgg cgctcaatgg ttcgaagccg cgccgtttgc aaaagccaaa gacgcttttg
      781 cgattggtat gaaaaaaaac tacaaatcta ttgcccgctc cttaggacta cgcgcggccg
      841 cccgctggca ctcatttcga tttccagcgg cgcctcgaac acctcttcga gtaaagcgcg
      901 ctcgtacact tccgcaggct ttcccacgcg caggcacttg cctttgtgga gcagcacgat
      961 gcgatcgcag aactcggcgg ctaaatcgag ttcgtgcgtg accaccagaa cggcgtagcg
     1021 attttcgtcc gccagccgac gcaaccgccg cagcaattcc acctttccgc cgatatcgag
     1081 atgctgcgtc ggctcatcga gcagtagcag cgacggccgc tgcgccagcg cgcgtgccaa
     1141 aatcacacgc tgtttttcgc cgccggaaag ccggtccatc catcgatcgc gaagcgcgtc
     1201 cgcgcccact tgggccagcg cgtttccggc gtgcaggcaa tcctccgcgg attcaaaacg
     1261 cagccgccgg ccgtaaggat agcgtccctg caatacgtac tcccacacgc gcagcggaaa
     1321 cagcagctca ctttcctgct gcaccagggc gatctgtttc gcgcgcgccc gcaaatcgat
     1381 ctggctcact tccacaccat caatttcgat ccgcccggat aatggcttgt ttactccggc
     1441 aataagcgtg agcagcgtgg attttccgct agcgttcgga cccaggatgg cgaccagttc
     1501 gcgctgctgc gcttcgaagc tcaccggacc cagcgcgaat ttcggcgcgg agcggtcggt
     1561 ggctgcgtat gcgtagcttg cctgctcgac tttcagccgc aatcccggtt gcctcgcggc
     1621 ggtcgcgctc gccgccaccg aggcgttgct tgccgccgcg ccgctcatcg cactctccgc
     1681 cgcatcagat aaatgaagac aggggcgccc gcgatcgccg tcatcgcgcc gaccggcaat
     1741 tccgtgggag ccacaactat ccgcgccagc gcgtccgcga gcacaatcag aattgcgccg
     1801 ccgagcgcgg atgtgggaat cagcagccgg tagtcggtcc caaacatcat gcgcatcagg
     1861 tgcggcacca gcagacctac gtatccgatc gctccgctca cagatacggc gaggccggta
     1921 agtattgaag ccgctacgta aaccactatc ttcactcgat tcacgttcac gccgaggtgc
     1981 cgcgcctctt gttcgcccgt gagaatcaga ttcaaatccg acgcggtagc gtaaatcgaa
     2041 cccacgccga gaaccaacac caggaaaagc catcgcgaat caaccgtggg tcgcgaagcc
     2101 agatcaccca taagccaaaa ggccatgccg cggaggtcgc gcccgctcag cgtggtcatc
     2161 atgaacatga tgacggccgc aagaaatgac gcagtcacaa ttccggccag caaaagcgtc
     2221 gcgctatcga gttgcccgcc gcgtcgaccc agaaaataca cggcggcggt tgtcacaccc
     2281 gcgcccgtga aagcagccac ttgaatggcg cccggcgtgt gcggcgcgac gatcaacgca
     2341 atgatcgcgc ccagtgcggc tccgctcgaa atacccagca cgtacggatc agccagtgga
     2401 ttccgcaaga gtgcctgaaa acccgcgcca gcggtcgcga gcgaagcccc tacgacaatc
     2461 cccagcaaaa tgcgcggcaa tcgaatctcg aatattacgt cgcggaattc cgcggggatt
     2521 gcgtcccagt ggtgtagaac gccattcacc agcgtcatca cgatatcgcg cacaccaatg
     2581 ggatatgcgc ccacgcgcag cgagatcacc atggccgcaa acagcacgat ggcaagccca
     2641 atacaaatcc atgccacgcg ctgcggcgtc agaggccgca ttcagggcac ctctccgcga
     2701 ttcgagtggc agcgcgcgat gccgatgacg tttggaattt atttccattt ggcatcgtac
     2761 gaattttgga aaacgcctcg ggatgcaact gccgcgcgag gtgttcgatc atatccacga
     2821 gtccaggcga cggccgtatc gcctcttcgc tgacgtcaac cacatgcccc tgctcgacgg
     2881 catccaaatc tctccacgct ggtcgggcgc gcaaatccgc gagctctgcg gcggctggcc
     2941 cgccatgatc attcgtgaag atgatgtagt ccggctgcag ccgcaccact tcttcgatgc
     3001 tcaggtgcgg ccatttctgc ttcgatatca gcacggattc cgcaccggcc catcgcagcg
     3061 cgtctgcaat gaaagtattc tgcccgatgg tctgcagcgg ctccagccac actacaaaca
     3121 gcacgtggac catcggccgt tcgctcagcc gcgcgcgcac tgcatcgagg cgctgttgca
     3181 gctcgtcaac gagatccgtt ccctgcttcg gcgcgccgat cgcatccgcc atgcgctgaa
     3241 cggaatcgag catcccgcga acggtttcgg gatccgtagt gtagaccgca attcccagat
     3301 tcttcagcgc gtcagccgtt tcaacacgat tgattgaagt ggtcgcgagc accaagtccg
     3361 gatgcagcgc cacaatggct tcgaggctcg gattctgcgg atcgcccaca tgcggcttca
     3421 attttgccgc ggccggcgtg tcgcagacgt tcgtgtcgcc gacgagccgg tcctcgagcc
     3481 ccagcgcgta aatggtctcc gtgagattcg gcgcgagtgt cacgacgcga ttcacctttg
     3541 cgggtatttt cacttctcgc cccacctcat cggtcaccat gcggctgcga tcagtttgcg
     3601 ccgcaatcat acgaggtgcg gagcttgtaa cgaatgcgac tgcaatcatc gttatccaaa
     3661 ctcgctctcg ccgcactcgg ttcaattctt gtctccaaca aaaaagcctc gcgaggatag
     3721 cgaggctcat tgccgcaccc tttctcgcga aggatgttgg ctttgcctgg cacaggtaca
     3781 ttcctgactt tcagcggcca ttcacgcggc gcgccgatca cagtggcggg accgtggccg
     3841 attttcacgg ccttccagat gcccgtgcca gttcaattgt cgctggcgag tctatgctgc
     3901 accttacttg gtgtcaatca gtctcgcttc agcggttctt ccacttcggg gcgcgctttt
     3961 ccaccagcgc gcgcaaacct tcctgcgaat cttcgagctc cgccaattcg ttcaagaaaa
     4021 ccttcatcga attgcggacg ccatcgcgca acggcaatcc gagactgccc atgatggctt
     4081 ttttcgccat cgtcagcacg ggtgcggatt gcgcggtcac tttcgcggtt acctcgtcaa
     4141 ttttcttctg cagcccatct tcgggcacca gccaattcac caaccccaat tcccgtgcgc
     4201 gctccgcgga catcgtttct cccgtcaaca caagttcgag cgcctgcttg ggaccgagaa
     4261 ggtacggcag aacggcggca gccagcggcg gaaatactcc cagcttgatt tcaggctgcg
     4321 cgaactttgc cttcggcgtg gcaatcacca gatcgcccaa cgccgccagc tcagctcctc
     4381 ctccgaatgc ggagccgttc accactacaa gcagaggctt cgaggtgtcc agcatcgccg
     4441 tgaatgcgcc gtgaaaagca tccaggagtt gaaatattcg ccgctgcgtg tactcgccga
     4501 tctcgatgcc gccgcaaaac gctttggccg ccgaatcgag aatgatcagc tttgtgttat
     4561 tgcgatcccc gagcgagttg atgcccccgg caagctcgct gagcatgcgc tcgttcagaa
     4621 gattgtggtc ggggcggtcc agcgtcagat ggccgacatc gccttcaacg cgcaccttta
     4681 cgaactgaaa gtcttccagt ttcggcgcga ctagcgccag ggttccgaga acttccactc
     4741 cgcgcgatgt ttgaggcatt tggaaatcgc tccaggcctg cgactcccct tggacgcggg
     4801 gacgtcttcg tccaaaggtt agaccgtttc gatttcgact gccatgccca atccgccgga
     4861 cacacacaat gtagcaaggc cccgccggcc agcc
//
LOCUS       DATNDT010000487         3310 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4162510, whole genome shotgun sequence.
ACCESSION   DATNDT010000487 DATNDT010000000
VERSION     DATNDT010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3310
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4162510"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1137
                     /locus_tag="VN788_16010"
     CDS             <1..1137
                     /locus_tag="VN788_16010"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HXU22088.1"
                     /translation="DGGKVIAFGGRAMGDDMPKYLNSPETPVYSKSNVLYHLDRAKES
                     LRQADFAILVEGYMDAIAVARAGFSNVVASCGTALADAQIKLLARFTRRVVVNYDPDA
                     AGQAATERSLMLLLEKEFDVRVLALPGGADPDKFLQQQGAAAYRKLLSQSPPYLDYLI
                     ARARRMDQTTAEGKLAAINFLMPFVQRIPNHLLRSEWASRIASELRVEAPVLREALRR
                     AAAERRSEVKPRAELLAPVVKPAERRLMQMLVEATGFRERLATEIAADELHRGLETER
                     IFEALIARAAAPADAAAIAAELGDRDRQILLEIVFADSSEAVWDEAESCLSVLRGRRV
                     EQELFELQKRIEAKPAGEELARLLKRRIELQRHLALQKTAVGGQ"
     gene            1236..2228
                     /locus_tag="VN788_16015"
     CDS             1236..2228
                     /locus_tag="VN788_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020133176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXU22089.1"
                     /translation="MTGPSPDKIMQLITGGWATSILGSGARHGIFTVLEANPATADEI
                     AKKTGISPRGARAVLDGLTGLGLLTLSEGKYRNTPEASAFLVKGKPSYFGAMAEVFLE
                     DFPTWQKMPDAVKSGTPSASNTSDVVDNPFWHALVPAIASLSFPVAQIAAERLGISKA
                     GAISWLDVGGGSGVWSAVWLGANKQAQGTQLDWPNVNKIAREFVSGFGVADRFHTIDG
                     DFHTADFGAAKYDFAIYAHIAHQESPQVNTEMFRKFRKALKPGGTLVVNDFVLNDDRT
                     GHPFAMLFASQMLVATKEGCTYRQSDYRDWLGQAGFKSVEIVPTPTPATLVFAS"
     gene            complement(2364..>3310)
                     /locus_tag="VN788_16020"
     CDS             complement(2364..>3310)
                     /locus_tag="VN788_16020"
                     /inference="COORDINATES: protein motif:HMM:NF018028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HXU22090.1"
                     /translation="LEPSAAQNVPVIRICPKCGALLMDDAEPCSFCDDSASDEKDELL
                     QPASIGDSVAEEDGEPEWRREVGRRLAEYRARRGYSAREEEPAGSHAQAGLPFHQTQR
                     VRKAPQVAEKKEAARPRTLARLRPVERLEISVQPELDFSAAAGERAHPQTALVPVATL
                     AERRNAAAVDILLILLTLGAFAGLFVGLIRSMGGEMVLDRLDAVMCGPVLFLFYSLYF
                     VVFTVFAGATPGMQARGLAIVRLDGHLPDTRQLTWRGFGYVLSGATLMLGFLWAYWDE
                     DAFTWQDRISQTYVTSSMPLVEPDAIEISAGQRRPLRK"
BASE COUNT          700 a         1072 c          912 g          626 t
ORIGIN      
        1 gacggcggca aagtgatcgc cttcggtggc cgcgcgatgg gcgacgatat gccgaagtat
       61 ctcaattccc ccgagacgcc cgtctactcc aagagcaacg ttctctacca tcttgaccgc
      121 gcaaaggaat cgctccggca ggccgatttt gcaattctgg tcgaaggcta tatggatgca
      181 attgcggtcg cacgcgccgg attctccaac gtcgtagcga gctgcggcac cgcgcttgcc
      241 gatgcgcaga tcaaactcct cgcccgtttc acgcgccgcg tggtggtcaa ttacgatccc
      301 gatgccgcgg gacaggccgc cactgaacgc tcgctgatgc tcctgctcga aaaagaattt
      361 gacgtgcgcg tgctcgcctt gccgggcggc gcggatcccg acaagttcct tcagcagcaa
      421 ggcgcggctg catatcgcaa actgctttcg caatcgccgc catatctcga ttacctgatt
      481 gcccgcgccc gccgcatgga tcagacgacg gccgaaggca agctcgccgc catcaatttc
      541 ctgatgccgt tcgtgcagcg cattccgaat catctgctgc gttccgagtg ggcgtcgcgt
      601 atcgcgtcgg agcttcgcgt cgaagcgccc gtattgcgcg aagctttgcg ccgcgccgca
      661 gccgaacgac gcagcgaagt caagccgcgc gccgaattgc tcgcgccggt cgtcaagccg
      721 gccgagcgcc gcctgatgca gatgctcgtc gaagccacag gttttcgcga gcgtctggcc
      781 acggaaatcg ccgcggacga actccatcgc ggcctcgaaa ccgagcgcat ttttgaagcg
      841 ctcatcgcgc gcgccgctgc gccggcggac gcagccgcaa ttgccgcgga gctgggcgat
      901 cgcgaccggc aaatcctgct cgaaattgtt tttgcggatt cttcggaagc cgtctgggac
      961 gaagccgaaa gctgcctcag cgtgctgcgt ggccgccgcg tcgaacagga gttgttcgag
     1021 ctgcaaaagc gcatcgaagc caaacccgcc ggagaagagc tcgcgcgtct tctaaagcgc
     1081 cgcatcgagc tgcaacgcca tctggccctg cagaaaacgg ccgtcggcgg gcagtgaggc
     1141 tgcaagaacc atcccgcaaa accctcatat tcgctagaac gaataaagat cgttcgatat
     1201 agttttgctg aacactttta aactcggagg catcgatgac aggaccttct cccgacaaaa
     1261 tcatgcagct catcaccggc ggctgggcca ccagcattct aggttcgggc gcacggcacg
     1321 gcatattcac cgtgctggag gcgaacccgg cgacggcgga cgaaatcgcg aaaaagactg
     1381 ggatttcgcc gcgcggcgca cgggcagtcc tcgatgggct caccgggtta ggcctgctca
     1441 ctttgtccga aggaaaatat cgcaatactc cggaggcatc cgcgttcctc gtgaaaggca
     1501 agcccagcta tttcggcgcg atggcggagg tctttctgga agatttccca acgtggcaga
     1561 agatgcccga tgcggtaaag agcggcacgc caagcgcctc gaacacttca gatgtcgtcg
     1621 acaatccttt ctggcatgcg ctcgttcccg ccattgcctc gctttcgttt ccggtcgcac
     1681 agatcgccgc tgaacgcctc ggcatttcga aagccggcgc aatttcctgg cttgatgtcg
     1741 gcggcggctc cggtgtttgg tcggcggtct ggctcggcgc gaataaacag gcgcaaggaa
     1801 cgcaactcga ttggcccaac gtaaacaaaa ttgcccgtga gtttgtttct ggcttcggcg
     1861 ttgccgatcg tttccacacc attgatggcg actttcacac cgccgacttc ggcgcggcga
     1921 aatacgactt cgccatctac gcgcacatcg cgcatcagga aagcccgcag gtcaataccg
     1981 agatgttccg caaattccgc aaggccctga aacccggagg cacgctcgtc gtcaacgact
     2041 tcgttctcaa cgacgatcgc accggccatc cattcgcgat gctctttgcc tcgcagatgc
     2101 tggtcgctac aaaggaaggc tgcacctatc ggcaatcgga ttaccgggat tggctggggc
     2161 aggcaggctt taaatccgtg gagatcgtgc ccactccaac gccggcaacc ctagtcttcg
     2221 cgagctaacg cgtccggaaa atggctcggg ctggaacggc ggggctgaag gagcaatcgc
     2281 gaattttaaa gcgccattcg cgccattttt ggcttttcaa agccacttaa gccgttttta
     2341 cggcgccaaa cgcgccaaaa ttgtcacttc cgcagtggcc ttcgctgccc tgccgaaatc
     2401 tcgatggcat ccggttccac gagcggcatc gacgacgtta catacgtctg ggagatccgg
     2461 tcttgccacg taaatgcatc ctcatcccag taagcccata gaaatccaag cattagcgtc
     2521 gcccccgaaa gcacatagcc gaatccgcgc cacgtaagct gccgcgtgtc cggcaagtgg
     2581 ccatcgagcc gcacaatcgc aagcccgcga gcctgcatcc ccggcgtcgc cccagcgaaa
     2641 accgtaaaca ccacaaaata aagagaataa aatagaaaaa gcacaggtcc acacatcacg
     2701 gcatcaagcc gatctagcac catctctccg cccatcgacc ggatcagccc aacaaacaat
     2761 cccgcaaaag cccccaacgt caacaatatc aataagatat cgacagccgc cgcgttccta
     2821 cgctccgcga gagtcgccac cggcaccagc gccgtctggg gatgcgccct ctctcccgct
     2881 gcagccgaaa aatccaactc cggctgcacc gaaatctcca ggcgctccac gggccgcaat
     2941 cgcgccagcg tgcgcggcct agcagcctct ttcttttcag caacttgcgg agccttgcgt
     3001 actctctggg tttgatggaa tggcaatcca gcctgcgcgt gagaacccgc cggctcttct
     3061 tcccgagcgg aatatccgcg ccgcgcacgg tattccgcca gccgacggcc cacttcgcgc
     3121 cgccattcag gctcgccgtc ctcctcagcc accgaatcgc caatacttgc gggctgcagc
     3181 agttcgtctt tttcgtcgga agcggaatcg tcgcagaacg aacatggttc cgcgtcatcc
     3241 atcaggagag cgccacactt cgggcagatt cgaatcactg gtacgttttg cgccgcggag
     3301 ggttccagtg
//
LOCUS       DATNDT010000488         5812 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_969935, whole genome shotgun sequence.
ACCESSION   DATNDT010000488 DATNDT010000000
VERSION     DATNDT010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969935"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1430)
                     /locus_tag="VN788_16025"
     CDS             complement(<1..1430)
                     /locus_tag="VN788_16025"
                     /inference="COORDINATES: protein motif:HMM:NF014589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase N-terminal
                     domain-containing protein"
                     /protein_id="HXU22091.1"
                     /translation="MIILGINAYHANAAAAIVVDGQLVAAVEEERLNRVKYAAGLPAR
                     AIQFCLNQAGAKLSEVDHIAIPRDPWARLGTKLRYAMRMPSFALDRARVIRRFAGIRE
                     EIAAAFEIDPSAIRAQFHRVEHHRAHLASAFFVSPFDRAAVLSADGLGDFASFMCGAG
                     EGPKITALGEVAFPHSLGIYYTALTQYLGFWKFGDEYKVMGLAAYGQPAFLDEFRRIV
                     RTDPPFSFRLGLEYFTHQNNGADMTWRDAARTPVIGRLFSAFLETRLGPARQPDEPLN
                     QRHYDLAASMQTALEEVLVACWNGLAERSGQTALCLAGGVAFNCVANGKVFDSTPFEH
                     VYVQPAAGDAGLSVGAAFEVHHNLGGARRFTMDHAYWGPVFSSSDIRAVVDRTFSSGE
                     ADISELAEAALVQSTARHIADGKIVGWFQGAAEWGPRALGNRSILADPRRHEMKDMLN
                     RRIKHREAFRPFAPSIIEEAVGEFFE"
     gene            complement(1475..3445)
                     /gene="asnB"
                     /locus_tag="VN788_16030"
     CDS             complement(1475..3445)
                     /gene="asnB"
                     /locus_tag="VN788_16030"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HXU22092.1"
                     /translation="MCGVCGVIGIESREAGEAAVRRMMDAMAHRGPDGEGTLFVPPIA
                     AGMRRLSIIDLPGGSQPVWNETQTLATVFNGEIYNFRELRKELENAGHRFRTHSDTEV
                     IVHAYEEWGECCVRRLRGMFAFAVVEMPQGPLARATHVFLARDRLGIKPLYYAFVEHK
                     LFFASEVRALLASGNVPARLAPGAIPAYLLFGAVCEPSTLVEGVTSLPPGYSLRISVD
                     ERSWPLAPTQYWDAESANETIAAGGSKESVFSTQQVRALLKDAVTSHLVADVPVGVFL
                     SGGIDSTAIAALASESQGGIQTFTVAFPDTEFSEAEQARLTARLLGTEHLELMLSDND
                     MLARFDEALAAFDQPSMDGINTFFVSWAAHQAGLKVALSGLGSDEVFGGYTSFRARSA
                     LACGSVAGRLLPRSLRRAGGGVMSLAGTTLVPPDRLRKALAAFQYPDLFPDSYFFTRL
                     VFTPGILAELQPRGSASWETLPWWRWLRDTTGQTKSMDSFTRVSWLELRSYLLNILLR
                     DTDCMSMSNSLEVRVPFLDGPLVEYVLSLPESLKAHSTQPKSLLIAALPDILPFHILS
                     QRKRTFTFPWQNWLRGALRKRVAAGLADWSPSLEPEVGSSFASGVWRDFLNGRTTWSR
                     PWSLYVLNEWVKRNLRGDRADAAGLREPAAVA"
     gene            complement(3450..4424)
                     /gene="sucD"
                     /locus_tag="VN788_16035"
     CDS             complement(3450..4424)
                     /gene="sucD"
                     /locus_tag="VN788_16035"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HXU22093.1"
                     /translation="MSILVDKGTRVIVQGLTGREGSFHSQQMIDYGTKIVAGVTPGKG
                     GTKKIGVPVFNTVTDAVRETGANAVMAFVPPPFAADAILEAADASLPLVICIAEGIPV
                     LDMVRVAAALRNSQTRLIGPNCPGIISPGQCKLGIMPGFIHKQGHVGVVSRSGTLTYE
                     AVGQLSSLGIGQSTCIGIGGDPIIGTNFVDVIQMFNDDPETHAIVMIGEIGGNAEEAA
                     ADFIKKHVKKPVVGFIAGQTAPPGRRMGHAGAIISGGQGTAAEKYAALRAAGIHTVES
                     PAEIGHAVAAVIGRRGARSDRKSAMRKKISSHQKKSPDEKKLAPVSKD"
     gene            complement(4421..5587)
                     /gene="sucC"
                     /locus_tag="VN788_16040"
     CDS             complement(4421..5587)
                     /gene="sucC"
                     /locus_tag="VN788_16040"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HXU22094.1"
                     /translation="MKIHEYQAKVILAKFGVSVPRGRVVRKKDEARAAAEELGLPVVV
                     KAQIHAGGRGKGGGVKVVTSADEAASFAEHILGANLVTPQTGSEGRMVRCLLVEEALD
                     IRRELYLSVLVDRVARAPLFMASIAGGMEIEEVANDRPESILREPIYPETGFQPYHAR
                     KIAFGLGLPADMINAAVPFLQSLYRAFLETDASLLEINPCVITADGRLMALDAKVNFD
                     DNALYRHPELRELRDLDEETPLEVEASKFKLNYIKLDGTIACMVNGAGLAMATMDIIK
                     LSGGTPANFLDVGGDASEERVKNAFRILLSDPHVKAVFINVFGGILRCDMLASGIVSA
                     AKELQIKAPVIVRMEGTNVKRGQEILRNSGLNFTVANGMKDGAEKAVALAGEAR"
BASE COUNT         1295 a         1703 c         1616 g         1198 t
ORIGIN      
        1 ttttcaaaaa actctccaac tgcctcttca ataattgaag gtgcaaacgg acgaaatgct
       61 tctcgatgtt tgatacgacg attcagcatg tccttcattt cgtgacgtct gggatcggcg
      121 agaatgctgc gattacccag agccctgggt ccccattcgg ctgctccctg aaaccagcct
      181 acaatttttc catcagcgat gtgtcgtgcg gttgattgaa cgagcgcagc ttcggcgagt
      241 tccgagatat ccgcctcgcc ggacgagaaa gtgcgatcca caaccgcccg aatatcagaa
      301 gaagaaaata ccggacccca atacgcgtga tccatcgtga atctgcgagc gcctccaaga
      361 ttgtgatgca cttcgaacgc cgcgccgacg gagagtcctg cgtccccggc tgcaggctgg
      421 acatacacat gctcgaacgg agtagagtca aaaaccttgc cattggcgac gcagttgaag
      481 gccaccccgc cagccaagca cagcgccgtt tgtccactgc gctccgcaag accattccag
      541 caagccacca gaacttcttc taaagcagtc tgcatactgg ccgccagatc gtaatgccgc
      601 tgattgagcg gttcatcagg ttgtcgcgct ggtccgagcc ttgtttcgag aaaggcggaa
      661 aagagccggc cgattaccgg agtgcgggca gcatcgcgcc aagtcatgtc ggcgccattg
      721 ttctgatgcg taaaatattc aagtcccaag cgaaaggaga atggtggatc cgtccggacg
      781 atacgccgaa attcgtcgag aaatgcaggc tggccgtagg cggccaggcc catcaccttg
      841 tactcgtcgc caaatttcca gaagccgaga tattgagtga gtgctgtgta atatattccg
      901 agcgaatgag ggaacgccac ttcgccaagc gcggttattt ttggtccttc ccccgcgccg
      961 cacatgaaac ttgcgaagtc gcccaaaccg tccgcagaga gcacagctgc ccgatcgaat
     1021 ggcgacacaa agaatgcgct cgcaagatgg gcgcggtggt gctcgacgcg gtgaaattga
     1081 gcccggatcg cactcgggtc aatttcaaat gcagcggcga tttcttcgcg aataccggca
     1141 aagcgacgaa tcacgcgggc gcgatcgagg gcgaacgacg gcatgcgcat ggcgtagcgc
     1201 agctttgtcc ccaagcgagc ccacggatcg cgggggatcg caatgtgatc cacttcggaa
     1261 agctttgcgc cagcctgatt gaggcaaaac tggatcgcgc gggctggcaa cccggcggcg
     1321 tatttcactc gattgagccg ctcttcctcg acagccgcga cgagttggcc atcgacgacg
     1381 attgccgcgg cggcgttcgc atggtaggca ttgatgccta ggatgatcat tgggttttag
     1441 atgcgacgct gttcacttgg gcgtcagtca gcggttaagc tacagccgcg ggttcgcgca
     1501 aaccggctgc atccgcccga tcgcctcgaa ggttccgctt tacccactca ttcaatacgt
     1561 aaaggctcca cggacgcgac catgtcgtgc gaccattcag gaaatcgcgc catacaccgc
     1621 tggcaaagct cgaaccgact tccggctcaa gactcgggga ccagtccgcc aggccggcgg
     1681 cgacacgttt ccgcagggca ccgcgcagcc aattttgcca ggggaatgta aaggttcgct
     1741 ttcgttgcga taaaatgtgg aaaggcaaaa tgtccgggag cgccgcaatg aggaggctct
     1801 tgggctgcgt cgaatgcgct tttagcgact ctggaagcga caagacgtac tcaacaagcg
     1861 gcccatccaa gaacggcacg cgaacctcaa gagaattgct catgctcatg cagtcagtat
     1921 cccgcagaag gatattcagc aggtaggaac gaagctccag ccacgacacc cgtgtgaaac
     1981 tgtccattga tttggtctga ccggtggtat cacggagcca gcgccaccaa ggcaacgtct
     2041 cccacgaagc agagccgcga ggctgcagct cggccagtat gccaggcgtg aagaccaaac
     2101 gtgtgaaaaa gtacgagtct gggaacagat cgggatactg aaatgcggct aaggcttttc
     2161 gcaaacgatc cggtgggact agcgtcgttc ccgcgagact catcaccccg ccgcccgccc
     2221 gacggagaga acgcggcaac agacggccgg ccacggatcc acaggcaagt gcagaccggg
     2281 cgcgaaatga ggtgtatccg ccaaatactt catcgcttcc taagccggaa agagcgactt
     2341 tcagtcctgc ttgatgagct gcccacgaaa cgaaaaaagt gttgatgcca tccatgctgg
     2401 gttggtcgaa ggcggcaagc gcttcgtcaa atcgcgcgag catatcgttg tctgagagca
     2461 tcaattcgag gtgttcagtt ccaaggagcc gcgccgtcag gcgcgcctgc tcggcctcac
     2521 taaattcggt atcaggaaag gccaccgtga atgtttggat tccgccttgc gattcactcg
     2581 cgagggccgc aatggccgtt gaatcgattc cgccactgag aaatacgcca accgggacat
     2641 cggccaccag gtggctggtg acggcgtcct tcaggagggc gcgtacttgc tgcgttgaaa
     2701 aaacgctttc tttagatcca ccggcggcga ttgtctcgtt tgcgctttcg gcatcccaat
     2761 actgggttgg tgcaagaggc cacgacctct cgtccaccga aatgcggagc gaatacccgg
     2821 gagggaggga agtaacgcct tcgactaacg tgctcggctc gcaaacggcg ccaaagagca
     2881 ggtacgccgg aatggcccca ggtgcgagac gcgcgggaac gttgccgctt gccaggagag
     2941 cacgcacttc cgatgcaaag aataacttgt gctcaacgaa tgcgtaatag agtggcttaa
     3001 tgccaaggcg atctcttgcc agaaagacgt gagtagcgcg ggcgagcggg ccctgcggca
     3061 tttccacaac cgcaaacgca aacattccgc gcaaccgtcg aacgcagcac tcgccccatt
     3121 cctcgtaggc gtgaacgatt acctccgtgt cggaatgagt gcggaaccgg tggcccgcgt
     3181 tttccagctc cttgcgaagc tcgcgaaagt tgtaaatttc gccgttgaag acggtcgcta
     3241 gcgtttgggt ctcgttccat acgggctggc tgccgccagg aaggtcaata atgctgaggc
     3301 gacgcatgcc tgctgcaatc ggcggaacga ataatgtgcc ttcgccatcc gggccgcggt
     3361 gggccatagc gtccatcatc cggcggacag ccgcttcgcc tgcttctctt gattcaattc
     3421 cgatgacgcc acatactccg cacacgagcc taatccttgg aaacgggcgc tagtttcttt
     3481 tcgtcgggag atttcttttg gtggctggag atttttttcc tcatcgcgga ctttcggtcg
     3541 gacctggccc cgcggcgacc gatcaccgcg gcaaccgcat ggccgatctc tgctgggctc
     3601 tctaccgtgt gaattccggc ggcgcgcagt gccgcatact tctccgcggc agtaccctga
     3661 ccgccgctaa taattgcacc cgcatgaccc atgcggcggc ccggcggcgc ggtttgtcca
     3721 gcaataaaac ccactaccgg ttttttcaca tgctttttga taaagtcagc cgccgcctct
     3781 tcggcattcc cgccgatttc cccgatcatc acaatggcgt gcgtttcagg atcgtcgttg
     3841 aacatttgta tgacgtcgac gaagttcgtt ccgataatcg gatcgccgcc aatgccgatg
     3901 caggtggatt gaccgatgcc aaggctcgaa agctgcccga ccgcttcgta tgttaatgta
     3961 ccgctgcggc tcacgactcc gacgtggcct tgtttgtgga tgaatccggg catgattccg
     4021 agcttgcatt gaccaggaga aattattccg gggcaattcg ggccgatgag ccgcgtctgc
     4081 gagttcctta gcgctgcggc gactcgaacc atgtcaagca cgggaattcc ttcggcgatg
     4141 cagatgacta gcggcaagct cgcgtccgct gcctccagaa ttgcgtcagc ggcaaaaggc
     4201 ggcggaacga atgccattac agcgtttgcc ccagtttcgc gaacggcatc cgtcacggta
     4261 ttaaacaccg gcacaccgat tttcttggta ccacctttgc cgggcgtcac gccagccacg
     4321 atcttagtgc cgtagtcgat catttgctgc gagtgaaagc tgccttcgcg gccggtaagg
     4381 ccttgcacga taacgcgcgt acctttgtcg acaagaatac tcatcgcgcc tcgcccgcta
     4441 gcgcaacagc cttctcggca ccgtctttca ttccattggc cacggtgaaa ttcagaccgg
     4501 aattgcgcaa gatttcctgt ccccgcttaa cgttcgtgcc ttccatccta acaatcacgg
     4561 gcgctttaat ttgcagctct tttgccgcgc ttacgatgcc gctcgccagc atgtcgcacc
     4621 gtagaatgcc gccgaagacg ttgatgaaca ccgcttttac gtgaggatca ctcaacagta
     4681 tgcggaacgc gtttttcacg cgctcctctg aggcgtcgcc gcctacatcg agaaaattcg
     4741 ccggggtgcc accactcagt ttgatgatgt ccatagtggc catagctagg ccagccccat
     4801 tcaccataca tgcaatcgtg ccatcgagct tgatatagtt gagtttgaat ttggaggctt
     4861 cgacctctag cggcgtttct tcatccagat cgcgcagctc cctcaattca ggatgacgat
     4921 agagggcgtt gtcgtcgaaa ttcactttgg cgtctagcgc cataagtcgg ccgtcggcgg
     4981 taatgacgca tggattgatc tccaagagag aagcgtctgt ttcgaggaac gcgcgataaa
     5041 gcgactggag gaaaggtact gcagcgttaa tcatgtcggc cggaagtccc aggccgaacg
     5101 caatcttccg tgcgtgatag ggctggaaac ctgtctctgg ataaatcggc tcacgcagaa
     5161 ttgattcggg acgatcgttc gcaacctcct cgatttccat gccgccagct atgctggcca
     5221 taaaaagagg cgcgcgggcc acgcgatcca ccaaaacgct gagatacaat tcgcgccgga
     5281 tatcgagcgc ttcttcaact agcaagcacc ggaccatccg cccctccgat ccagtttgcg
     5341 gggtaaccag attagcgccc aggatgtgct cagcgaacga cgcagcctca tcggccgacg
     5401 tgacgacctt cacgcctccg cctttgccgc gcccaccagc gtgaatctga gccttgacaa
     5461 ccaccggcaa gcccagctcc tctgccgccg cgcgcgcctc atccttcttg cgcaccaccc
     5521 ttccgcgagg cacagaaacg ccgaattttg cgaggattac tttcgcttga tattcgtgaa
     5581 tcttcattgc gggctcgaca ccggcaaaac caggcttgag aaatgctaga atttatgaac
     5641 caataacgca cttcagtgta cttgtaagct gcgtgacggg caaggtagca aaaagaatgc
     5701 tgcacgaggc ggttctaaaa gtcgacgcga aaacggacct gtcgcgttcc gaagctgagg
     5761 cagccatgga ggagatcctc tccgggcgcg ctacaaacgc gcatattatc gg
//
LOCUS       DATNDT010000489         9089 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1854222, whole genome shotgun sequence.
ACCESSION   DATNDT010000489 DATNDT010000000
VERSION     DATNDT010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1854222"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1188
                     /locus_tag="VN788_16045"
     CDS             295..1188
                     /locus_tag="VN788_16045"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HXU22095.1"
                     /translation="MNDYLLSLILGIVEGLTEFLPVSSTAHLRISEALLHISLSSGYW
                     KMYTIVIQLGAILCLPIYFRERIAKFLSTFPRGERGDRTVWTHPLSLVIIAFIVTAIP
                     SLLLVKIIGKHLESLYIMGSSLVIGGVIMWIVDAVHAKWEAAGPGVPSSRIRTWHMEE
                     MGFGQAVWVGICQILSGVFPGTSRSMSTITGGQLAGMSRASALEFSFFVSMPVMAAAT
                     GYDLLKSVYGKGANAVGVSGIDAHGWIVLGIGFVVSFIVAYGAVAWFMAWVRRHGFVP
                     FAIYRIIAGAAVLFWIAKLSS"
     gene            1346..2095
                     /locus_tag="VN788_16050"
     CDS             1346..2095
                     /locus_tag="VN788_16050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HXU22096.1"
                     /translation="MSGHSKWATIKHKKAATDARRGKIFTKLIRELTIAARTGGSDPD
                     TNPRLRTAILAAKGENMPNDNIERAIQRGTGGIEGETLDEVTFEGYGPGGVGVLVQVV
                     TSNRNRIVSEIRHMFSKNGGNMAETGAVGWMFERKGDIVVPKDQADEEKMMGVVLDAG
                     AEDLRDDGSSWEVTTPPEVMEKVREALAAAQITPSSAEVAWVPKNNVKLSGAQAQQML
                     RMVETLEDHDDVQHVYANFDIDEKEIAAMAG"
     gene            2210..2695
                     /gene="coaD"
                     /locus_tag="VN788_16055"
     CDS             2210..2695
                     /gene="coaD"
                     /locus_tag="VN788_16055"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020707302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HXU22097.1"
                     /translation="MTSPVTAIYPGSFDPVTNGHLDLIARGAMIFDKLIVAVARNLDK
                     DPLFQVKERVEMLEAVTFDWKNVEVEIFEGLLMDYARARNATVVLRGIRAVSDYEYEL
                     QMAMMNRKIEPQIETVFMLPGEAYSYLSSRLVKELARLGGPVKGLVPPLVEERLRSKV
                     S"
     gene            2839..4050
                     /locus_tag="VN788_16060"
     CDS             2839..4050
                     /locus_tag="VN788_16060"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HXU22098.1"
                     /translation="MEPATVVKTEETFRLADRVNRISPSPTMVVLQAAEQLKSQGIDV
                     ADFGAGEPDFPTPEHIKRAAIQALDENRTKYTPVPGIPALRQAICEWHARELGSSYQP
                     AECVVNVGGKHSIFNAVCCLINSGDEVIIPSPYWVSYPDIVKYAGGKPVILTTHAEDN
                     FVLRAVDLEKLITPRTRMVIADSPSNPSGAVIPPEEFAKVLAVCQRHGIWLLADECYS
                     HFSYGDAKPFSIASIPGSKSGVIIVGSFSKTFAMTGWRLGYVLAPQPLIAAITKLQGQ
                     STSNPTSITQYAALAAMRGPMDSVAKMLEEYARRRERIVTGLRAIPGVTCTAPQGAFY
                     VFPNISAHYNADMPNDMVVSKLLLEREHVALVPGEAFGAPGFLRLSYATSLDRIDEGL
                     RRLGRFFGQAR"
     gene            4146..5063
                     /locus_tag="VN788_16065"
     CDS             4146..5063
                     /locus_tag="VN788_16065"
                     /inference="COORDINATES: protein motif:HMM:NF042587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I phosphomannose isomerase catalytic
                     subunit"
                     /protein_id="HXU22099.1"
                     /translation="MVNLAEPIGEAWMTGNECRFAGGPFSGKKLGDVWPVISAEWKGT
                     RLSDGGSFPLLIKFIFAEDKLSVQVHPDDEYASQHERAAGGCGKTEMWYAVRARSGAE
                     VLVGLKPSVTRESFERAIADGTAENCIERLPLAAGDAVFVPARTAHTIGPGLVLCEIQ
                     QNSDLTYRVYDYNRRDAKGRARELHVEKALDVIRFGEQRGGKIQASQVHHGPVAETRL
                     AACEYFATERWAFSERIECVTTREHFDVLIFLEGNGSIRWGENHETYAAAQVWIIPAA
                     LGKYELHPNAENTAQPSATSLLRTHVPSK"
     gene            complement(5534..8968)
                     /locus_tag="VN788_16070"
     CDS             complement(5534..8968)
                     /locus_tag="VN788_16070"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXU22100.1"
                     /translation="MQSYVSSTGIVLQASCFLLAAFFVFLYRRKRQGYLLAWSAAWFL
                     VGLHFLFSALGVWLYSDEKWLGWLGAFNESVFAFAGLAFYCASRQYAGLKVPKWVSAA
                     GLVAIIWSIAHARYSVGVPAVVLGVGLIFLLVAHTFWHEGRKQEARADVLLGFTFAGW
                     GLLCISTLLHSRWVFLQKLHLLPAIILPELFACVLMVMSVYEEERRRAERNMLALSNL
                     NLVTGSFVGGEIQKMLAQALDRVLSVVRVPSGALCLQPGETSGSGASVIVAGLNDSFS
                     AAIQESELNEYFINLVARLGGLVVFRDLTRDASWRALEKDDAFRRIRQLLLDQGLRTI
                     VGISLQAKERVFGGLLLGTPDNRSFGPAERRLLLALGQQIGMAVENSYLIRQTSRRSE
                     ELHILNEIGRALSSTLDLDKLLERIYSEIRRVVDASSFFIAYHDQDTGEVRFEIEVVD
                     GKRLPKRSRPVANRLAEYVVRTEQPLLIRERFCEEAERLGFETQQPVGSICAVPLILY
                     DRPVGVMAVHSQKERVFDAGHVEFLRVLGSEAGIAIENARLFAEEQKKSRQLTLINNI
                     SNHAITTLDPEEMLGKIAAEMEKHLSYNHIGIAILDYSAKELVVQADAGLRREAVGRR
                     LLLGEGLIGQVARAGQMGIIREAGPSTPRLVLPDSAASVALPVTYGEQFLGVLYVETS
                     EPCDFSQQDVALLRTLADLFAGALHNTLTFQKAQEQAITDGLTGVKTHRFLMEALSSE
                     WKRSTRASRPLALVLMDLDRFKFVNDFYGHLEGDVVLQRVGQILEQNCRRSDVVARYG
                     GDEFVILMPETTIEQARQFANKLRSWIASDPILRDKNITASFGIAAFPLHGSSPQELI
                     QVADSSMYLSKHQGGNLVSSAEQGDPNDRKRWKKDVLEAYLGVTLKRLFSTGPEAFEE
                     IYRRLEQFTRSLREQGGDSDGNALPPSVVETVTSLAYAIDAKDHYTQGHSRKVSAYAV
                     MIARELGLGETEIEEIRLAGLLHDIGKVGIPEAILNKSGPLDAFEWETMKTHTDLGAR
                     ILETLKPMARIREMVRHHHEFYDGTGYPCRLEGENIPHGARVIAIADAYDTITSARTY
                     KKARTAEDAFAELERCAANQFDPALVRIFIDTMRRAPRPTLEAVASASGHAGTSSY"
BASE COUNT         1886 a         2800 c         2553 g         1850 t
ORIGIN      
        1 gtccgcgaag tggcgctcga gacggcaacc aacaatgcct gcgccattgc cttctggaaa
       61 aagcacggct atcttacgga cggggtgaaa aaaaactact atccgggggg catcgacgcc
      121 tactctatgc gtaagcaaat cgcattgaca tccacctgat tcgccttcgt agcgccggag
      181 tctcaccgca tctggcgcta caccggccgt ttgcagcctg cgcgaataat ttttttcctg
      241 agcggaggct gcgcgtaatg agcacgcgtt tcccgaattt attgtggagg aattttgaac
      301 gattatttgc tctctctcat tctcggcatc gttgaaggtc tcaccgagtt tttgccggtt
      361 agttccacgg cgcacctgcg catcagcgaa gcgttgctcc acatcagcct aagcagcggc
      421 tactggaaga tgtacaccat cgtgattcaa ctgggggcaa tcctctgcct ccccatttac
      481 tttcgcgaac gaatcgccaa attcctatcc acgtttcccc gtggcgagcg cggcgaccgc
      541 actgtttgga cgcatccctt gagcctcgtc atcatcgctt tcatcgtcac cgcgattccg
      601 tcgctgctgc ttgtcaagat catcggtaag caccttgaga gcctctatat catgggaagt
      661 tccctggtaa tcggcggtgt catcatgtgg atcgtggacg cggtgcacgc aaaatgggaa
      721 gctgccggcc ccggtgttcc aagcagtcgt atccgcacct ggcacatgga agaaatgggc
      781 tttggccagg ccgtatgggt cggcatctgc cagatccttt ccggcgtatt cccgggaacg
      841 tcgcgttcga tgtccacgat caccggaggt cagcttgccg gaatgtcgcg cgcctcggcc
      901 ctcgaattct cgttctttgt gtcaatgccc gttatggccg ccgccaccgg ctacgatttg
      961 ctgaaatccg tctatggtaa gggcgcgaat gcggtgggcg tctccgggat agacgcgcac
     1021 ggctggatcg tcctcggcat cggcttcgtt gtctcgttta tcgtcgccta tggcgcagtc
     1081 gcctggttca tggcctgggt caggcgccac ggcttcgtcc cattcgccat ctaccgaatc
     1141 atcgccggtg ccgctgttct gttctggatt gcgaaactgt cgagctgaat cgtctcgacc
     1201 agcccgtttc ataatccagc gccgtgcgat cgaaattgcc gcgcctttca agccgttgcc
     1261 ttgcctgttt cccacccggc tgctaaactg tcgcggcaag ggctaagctg ttgctcctgc
     1321 cacacctcct ctaaaaggtc gccttatgtc cggacattcg aaatgggcca caatcaagca
     1381 caagaaagcc gccactgacg cgcgccgcgg caaaatcttc actaaactca ttcgcgaatt
     1441 gaccatcgcc gcacgcactg gcggctccga tccggacacc aacccccgcc tccgcaccgc
     1501 catcctcgcg gccaagggtg aaaacatgcc caatgacaat atcgagcgcg ccattcagcg
     1561 cggtaccggt ggaattgagg gcgagacact ggacgaagta acctttgaag ggtatggccc
     1621 cggcggagtc ggcgtcctcg tccaggttgt caccagcaat cgcaatcgga tcgtcagcga
     1681 aatacgccac atgttttcca aaaatggcgg caacatggct gaaaccggcg ccgtcggttg
     1741 gatgttcgaa cgcaagggtg acatcgtcgt ccccaaagac caggcggacg aggaaaaaat
     1801 gatgggcgtg gttctcgatg cgggcgcaga agatctccgc gacgatggct cgtcctggga
     1861 agtcacaact cctcccgaag tgatggaaaa agtgcgcgag gcgctcgctg cggcgcaaat
     1921 cacgccgtcc tccgccgaag tcgcctgggt gccgaaaaat aatgtcaagc taagtggcgc
     1981 tcaagcccag cagatgcttc gcatggtcga aactctcgag gaccacgacg acgtgcagca
     2041 cgtttacgcc aactttgaca tcgacgaaaa agaaatcgcc gccatggccg gttagttcag
     2101 caggacctcg tcgcgatttc gtcgcacgtg gttttcgtgg ttgcgctttc aagagttgcg
     2161 gtccgcggcg caattctagg ttagaatccc tcaccggatg ccctcccaaa tgactagccc
     2221 cgtcacagcg atctatcccg gctcgttcga tccggtgacc aacggccacc ttgacctaat
     2281 cgctcgcggc gcgatgattt tcgataagct gattgtcgcc gtcgcgcgca acctcgacaa
     2341 agacccgctt ttccaagtca aggaacgcgt cgaaatgctc gaagccgtca ctttcgattg
     2401 gaaaaacgtc gaggtcgaga ttttcgaggg cttgctgatg gattacgcca gggcgcgcaa
     2461 cgcaacggtc gtcctcaggg gcattcgcgc cgtttccgat tacgaatacg agctccaaat
     2521 ggccatgatg aatcgcaaaa tcgagccgca gatcgaaacc gtgttcatgc tccccggcga
     2581 agcatatagc tatctcagtt cgcgcctggt gaaggaactc gccaggctcg gcggacctgt
     2641 aaagggcctg gtgccgccgc tagttgagga acgcctgcgg tcaaaagtca gctagctgac
     2701 aatggatccg cgatgccgcc cctcgaccgt ccagccactt tggcagcacg catttaatcg
     2761 cttaaatttt ctggttctga cgcaatgagc taaaatggat tcgctcgttg atccgcaacc
     2821 cgcgctaaag gataggaaat ggaaccggcc acagttgtga aaacagaaga gacctttcga
     2881 ctcgccgacc gcgtgaatcg cattagtccg tcgcccacaa tggtggtgtt gcaggccgcc
     2941 gagcaactga aatcccaggg catcgacgtc gctgactttg gcgcgggcga accagacttt
     3001 ccaacgccag agcacatcaa gcgcgccgcg atccaggcgc tcgacgaaaa tcgcaccaag
     3061 tacacgcccg tgccgggaat tcccgctctg cgccaggcca tctgcgaatg gcacgcgcgc
     3121 gaactcggct cgtcatatca acccgccgag tgcgtggtca acgtgggtgg caagcattcc
     3181 atcttcaacg ccgtctgctg cctgatcaat tccggcgatg aggtcatcat tccctcgccg
     3241 tattgggtca gctatcccga catcgtgaaa tacgccggcg gcaagcccgt cattctgacc
     3301 actcatgccg aagataattt cgtcctgcgt gcagtcgacc ttgaaaagtt gatcactccg
     3361 cgcacgcgca tggtgatcgc ggattcgccg agcaatccct ccggcgccgt gattccaccc
     3421 gaagaattcg cgaaagttct cgccgtctgc cagcggcacg gcatttggct gctggccgac
     3481 gagtgctatt cgcacttcag ctacggtgac gccaagccat tctcgatcgc cagcattccc
     3541 ggctcgaaga gcggagtcat catcgtggga tcgttttcca agaccttcgc gatgacgggt
     3601 tggcgcctcg gctacgtgct cgcgccccag ccgctgatcg ccgccatcac caagctgcaa
     3661 ggccagtcca cctcgaatcc cacgtcgatc acgcaatacg cggcgctggc cgccatgcgc
     3721 gggccgatgg attcagtggc caaaatgctg gaggagtacg cccggcgccg cgaacgtatc
     3781 gtcaccggcc tgcgcgctat tcccggcgtc acctgcacgg ctccccaggg agcgttctac
     3841 gtcttcccga atatttcggc gcattacaat gccgacatgc ccaacgacat ggtcgtctcg
     3901 aagttgcttc tcgagcgcga acacgtagcc ctcgtccccg gggaagcctt cggcgcaccc
     3961 ggcttcctgc ggctttccta cgccacgtcc ctcgaccgca tcgacgaggg cctgcgccgg
     4021 ctcggccgct tcttcggtca agcgaggtag cgatcagcga agcatctcta tgcccggtgc
     4081 gcttggagcc tgtctttagc cctcgcccct ggggacccct ttcgctggcg ccgtttttcc
     4141 ctgcaatggt gaatctcgct gagccgatcg gcgaagcctg gatgacaggc aacgagtgcc
     4201 gcttcgccgg cggtcccttt tctggtaaga aactcggcga cgtctggccc gtcatctccg
     4261 ccgaatggaa gggcacccgc ctcagcgatg gcggctcctt ccctctgctc atcaaattca
     4321 tcttcgcgga agacaagctt tccgtacagg tccatcctga cgatgaatat gcgtctcagc
     4381 acgagcgggc cgctggcggc tgcggaaaaa cggagatgtg gtatgccgtc cgcgcgcggt
     4441 caggcgccga agtgctcgtc ggactgaagc caagcgtcac tcgcgaaagt ttcgagcgcg
     4501 ccatcgcaga tggcacggcc gaaaattgca tcgagcgcct tcctctggcg gcgggcgacg
     4561 ccgtttttgt tccggctcgc accgcgcaca ccatcggtcc cggactcgtg ctctgcgaaa
     4621 ttcagcagaa ctccgatctc acctatcgtg tctatgacta caaccgccgc gatgcaaaag
     4681 gccgcgcacg cgaattgcac gtcgagaagg ccctcgatgt gattcggttc ggcgaacagc
     4741 gcggagggaa aatccaggcg tcgcaagtcc atcacggccc ggttgccgag actcgcctcg
     4801 ccgcttgcga atatttcgcc accgaacgat gggcatttag cgaacgtatc gagtgcgtca
     4861 ccacacgcga gcacttcgac gtgctaattt tcctcgaagg caacggaagc atccgctggg
     4921 gcgaaaacca cgaaacctac gcagccgccc aagtctggat tattcccgcc gctctaggaa
     4981 agtacgaact gcatcctaac gccgagaaca ctgcgcagcc atcggccacg tccctcctac
     5041 gcacccacgt ccccagcaag tagcagtcat cgtcattccg agcggagttt cgcgcgtttt
     5101 atctttcgcg cggtttatgc gcgcgcgaaa cgcagtcgag gaatctctct ttcgatcgag
     5161 cagcaccggg cactcaacgc ggaggcatgt ggagtgcagg agttcccgcc attccgagcg
     5221 ggggcgcgcg ccgtcattga gagccgagtt tgtagcatcg tcattccgct tgccctgacg
     5281 caggaaggga gcggagtttc gcgcgtttta tctttcgcgc ggtttatgcg cgcgcgaaac
     5341 gcagccgagg aatctctctt tcgatcgagc agcgccgggc attcaacgcg gaggcatgtg
     5401 gaatgcggga gctcccgcca ttccgagcgg gggcgcgcgc cgtcattcca agccgagttt
     5461 gtagcgccgt cattccgctt gccctgacgc aggaagggag cggagtttcg cgcctttcac
     5521 gccaacagac agatcaatat gaagaagttc cggcgtgacc cgaagcgcta gccaccgctt
     5581 cgagcgtggg gcgcggggcg cgacgcatcg tatcgatgaa aatccgaaca agcgccgggt
     5641 caaattggtt cgcggcgcag cgctccagct cggcaaaggc atcttcggct gtgcgggcct
     5701 tcttgtaggt gcgggcggaa gtgatggtgt cgtaggcgtc cgcgattgca atcacccgcg
     5761 cgccgtgagg gatattttcg ccttcgaggc ggcaagggta gccggtgccg tcataaaact
     5821 cgtgatggtg gcgaaccatc tcccggatgc gggccattgg cttcagagtt tcgaggattc
     5881 gcgcgccgag atccgtgtgg gttttcatcg tttcccactc gaacgcatca agcggccccg
     5941 acttgttcag gatggcttcg gggattccaa ccttgccgat atcgtgcagc agtccggcca
     6001 gcctgatttc ttcgatttcc gtttcgccaa gacccagttc cctggcgatc atcacggcgt
     6061 atgcggagac ttttcgggaa tgcccttgcg tgtagtggtc tttggcgtca atagcgtatg
     6121 cgagtgacgt gacggtctcg actaccgacg gcggcagagc attgccgtcc gagtctcctc
     6181 cttgctctct aagcgaacgc gtaaactgct cgagccggcg atagatttcc tcgaaggctt
     6241 ccggcccggt ggaaaatagg cgcttgagtg taacgcctaa gtaggcttcg agcacatctt
     6301 tcttccatct ttttctgtca ttgggatcgc cctgctcggc gctggagact agattgcctc
     6361 cctgatgctt ggaaaggtac atcgacgagt ccgccacttg aatcagctcc tgcgggctgc
     6421 tgccgtgaag cgggaaggcg gcgataccaa agcttgcggt gatgttctta tcgcgcagta
     6481 ttggatcgga cgcgatccag ctgcgcagtt tattggcgaa ctggcgggcc tgttcgatcg
     6541 tcgtctcagg catcagtatc acgaattcgt ccccgccata ccgcgccacg acatccgacc
     6601 ggcggcaatt ctgctccagg atctgcccaa cgcgctgcag taccacatcg ccttcgagat
     6661 gcccgtaaaa atcattgacg aatttgaagc gatcaagatc catgaggacc agcgcgagcg
     6721 ggcggctggc gcgcgtggac cgtttccatt ccgaggacag cgcctccatg aggaagcgat
     6781 gcgtcttgac gcccgtaagg ccgtcggtaa tggcctgctc ctgcgctttc tgaaacgtaa
     6841 gtgtgttgtg caaggcgccc gcgaagaggt cggctagggt gcgcaggagc gcgacatcct
     6901 gctggctgaa gtcgcatggc tccgatgtct ccacgtaaag aacgcccagg aactgctctc
     6961 cataagttac cggcagcgca acggacgccg ccgaatccgg cagcaccaat cgcggagttg
     7021 acggaccggc ttcgcggatg attcccatct gtcccgcgcg ggcaacctgg ccgataaggc
     7081 cttctccgag cagcagacgc cggccaaccg cttcgcgccg cagccccgca tcggcctgta
     7141 ccacaagttc cttggcggag taatcgagaa tcgcaattcc tatatgattg taggagaggt
     7201 gcttctccat ctccgcggca attttcccca gcatctcctc aggatcgaga gtggtaatgg
     7261 catgattcga aatattgttt atgagcgtaa gctgcctgga ctttttttgc tcttcggcga
     7321 acagccgggc gttttctatc gcaatgcccg cttcgctgcc tagcacgcgc agaaactcca
     7381 catggcctgc gtcgaacacg cgttccttct ggctgtggac ggccatgact cccacgggac
     7441 gatcgtaaag gatcagaggt acagcgcata ttgagccgac gggttgttgg gtttcgaatc
     7501 ccagtcgctc cgcttcttcg cagaagcgct cgcgaatcag aaggggctgc tcagtgcgaa
     7561 ccacatactc cgccagccgg ttggcaaccg gtcgcgaccg cttcggcagc cgttttccat
     7621 ccactacctc gatctcgaag cggacctcgc cggtatcttg atcatggtag gcgatgaaga
     7681 agctgctggc atcaaccacg cgacgtattt cggaatagat gcgctccagc aatttgtcga
     7741 gatccagagt ggagctgagc gcgcgcccga tttcattgag gatgtgcagc tcctcgctgc
     7801 ggcgcgatgt ttggcgaatg aggtagctat tttcaacggc cataccaatc tgctggccga
     7861 gcgcgagaag caggcgccgt tcggcggggc cgaagctgcg attgtctgga gtgccaagca
     7921 gcaggccgcc aaagactcgc tccttggcct gcaagctgat tcccacgatc gtccgcagcc
     7981 cctggtccaa gagtaattgc cggatacgcc ggaaggcgtc atctttttcc agcgctctcc
     8041 agctcgcatc ccgcgtcagg tcccgaaaaa ccaccagtcc gccgagccgt gcaaccaagt
     8101 tgatgaaata ctcattcagc tccgattctt gaatggcggc ggaaaaggaa tcattcaggc
     8161 cggccacgat caccgaagcg ccgctgccgc tggtttcacc cggttgcaag cacagagcgc
     8221 cggagggaac gcggacaaca ctcagcaccc ggtccagggc ctgggccagc attttctgaa
     8281 tctcgcctcc cacaaagctc cccgtcacca gattcagatt ggaaagagcc agcatgtttc
     8341 gctcggcccg gcgccgttcc tcttcgtaaa ccgacatcac cataagcacg caggcgaaaa
     8401 gctcgggcaa aataattgcc ggcagtagat gcagcttttg caagaaaacc catcgagaat
     8461 ggagcaaagt cgaaatgcag agcaggcccc agcccgcgaa cgtgaagccc agcagcacat
     8521 ccgcccgagc ttcttgcttt cggccttcgt gccaaaacgt gtgcgcaacc agaagaaaaa
     8581 tcaatcccac gcccagcacc acggctggca cgcctactga atagcgagcg tgagcaatgg
     8641 accaaatgat cgccacaagg cccgcggcgc ttacccattt cggcaccttc agcccggcgt
     8701 actggcgcga cgcgcagtaa aaggcaaggc ccgcgaaggc aaagacggat tcgttaaagg
     8761 cgcccagcca acccagccac ttttcatcac tgtagagcca cacgcccagg gcgctaaata
     8821 aaaaatgcag gcccactaag aaccacgcgg cagaccacgc aaggagatac ccctggcgtt
     8881 tccggcggta aagaaacacg aagaacgccg caagcaggaa gcagcttgcc tgaagcacta
     8941 tccccgtgga actgacatag ctttgcaaat tctatgctcc gcgtttagtc ggggccgttc
     9001 tgaggagaat agcagaaagc ccgagaatca cccgagcacg tctccgacca gtttcttgga
     9061 ggttaacgta gaagaactca ctgaccccg
//
LOCUS       DATNDT010000490         8396 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2183025, whole genome shotgun sequence.
ACCESSION   DATNDT010000490 DATNDT010000000
VERSION     DATNDT010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8396
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2183025"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..837)
                     /gene="thiC"
                     /locus_tag="VN788_16075"
     CDS             complement(<1..837)
                     /gene="thiC"
                     /locus_tag="VN788_16075"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HXU22101.1"
                     /translation="MNGGARNFSQMHFARKGIITEEMQYVALREKISPDAVRDEVARG
                     RAIIPANIHHRNLEPMGIGVAFGCKINANIGNSATTSNVSEELEKLHRAVHYGSDTVM
                     DLSTGGDIAMIRKAIIQASPVPIGTVPIYEALSRVRRAEDLTINVMLEVIEEQAEQGV
                     DYMTIHAGVLREFLPLVRNRITGIVSRGGALLAQWMSFHKAQNFLYENFDAICKIFQK
                     HDVSFSLGDGLRPGCLADASDEAQFSELRVLGELTKKAWEYDVQVMIEGPGHIPMDQI
                     ELQ"
     gene            1092..1481
                     /locus_tag="VN788_16080"
     CDS             1092..1481
                     /locus_tag="VN788_16080"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXU22102.1"
                     /translation="MRCMGPKFLLAAILLAAIPSAAFAHHGTSHYDLGKTITLMGTVT
                     GFDWGNPHCLVYIDVKADNDKVQHWTLELASPYTMSRAGWTRNSLMVGDMILAQTHPA
                     RNGITLGISATSRSIMKFVINGKPLRM"
     gene            complement(1539..5105)
                     /locus_tag="VN788_16085"
     CDS             complement(1539..5105)
                     /locus_tag="VN788_16085"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU22103.1"
                     /translation="MKHATRTLLRIAFIGILVFCCKQVWATTGGSISGTVTDENGGVI
                     PGASVTVRNLDTAVAQTTTTNGEGFYAFTNLPVGRYELEIIRDRFKPYKRTGLVIDVN
                     TQLRVDQVLTIGEQSQEVVVSGTAAQVETESTQVGQVVTSTEITGVALNGRSFTDLLA
                     LQPGIVPMSTQQPNSIVMAGATVAIAPSGDLNPGNQSISGQREDANGYMVNGGDVKEL
                     MNGGTTIVPDLDSIAEFRILTNNFDAEYGDYSGGIVNVVTKSGTNEIHGSGFEFLRNT
                     VLDARNYFSPTRAFFRQNQFGGTVGGPIKSGKVFFFADYQGTRMAQAIDTGLISVPSL
                     AYRSGDFTTGPNGIGPSPLIGAVGGANLAGILTGELGYPVSSNEPYFTPGCTSSTCVF
                     PGAMIPQAAWSAPAKSLLNFIPPPNDGPATFTTASQGKVLRDDKASFRIDGNNHRWGL
                     LSAYYFFDDFTLNNPYPTGQGGASVPGFNALNTGRSQLVNLADTKTFSGANVNEFHFS
                     FMRSANAVGQPVGGIGPSLESQGFTTCPGSTRTPGCAGIFALAPAIEGIENTIFNSFV
                     VGVPITNLAQANNTFTVSDNFSTVRGSHTIKAGFELSVEQINVNPNPTFNGSFLFSGT
                     ETGLDFADFLIGVSTNYNQADSQHYWGRHKYPAAFAEDSWRIKPTLTLNFGLRWELMQ
                     YWSEKQNQVPTFILGEQSKVYPTAPTSIVYATDAGVPSTLVPQRNRFAPRIGLAYSPT
                     KSNGILGKIIGGAGKTSIRTGYGVFYSVIQGNTIAFDEPQPPYGLSYTGNNTLFATPF
                     ITASDGSAHVNPFPLTFPPLNASVHHPNPTIDFSQFEPIAGMTAPPPSNTFPYAENYF
                     LSVERQLPGGTVLSLNYVGSQAHHLLAVFSANPGNAALCLSLSQPSDVAPGSPTCGPG
                     GEDATYITAGGTTINGTRSPLGSAFSNDDYEGSVASSNYNSFQASLRRSGKTLDLMFG
                     YTYSKSIDQASSLADILDPFNFNATRGLSAFDLRHNFVATYEYRLPFDRLTSRASMIT
                     QGWAISGITRASSGFPVTISTDLDNSLMGSLPNGVNNHSADLPDFVGGPLRLNGNPRN
                     GLPYFNTSLFGPNALGTFGNSRRRFFYGPGLFNTDLALSKSFQISESKTLQFRLEAFN
                     VFNHTQFFGPAAVNGDFSSALFGQVVNAAPPRLMQAALKFMF"
     gene            5336..6151
                     /locus_tag="VN788_16090"
     CDS             5336..6151
                     /locus_tag="VN788_16090"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009280281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HXU22104.1"
                     /translation="MSLTTLTLMAAITLLLGSSFAQQPPAANSQPSATAKTQKTPAAK
                     THVAPGTKNSTSATKSNTGPALTTDKEKDSYALGLNIGRGLSKQPVDLDAAAVARGLK
                     DALSGSKSLLTDEEAAADLAQLKDQAQKASDAKMQELGAENQKAGAAFLAENKTKEGV
                     VTLPSGLQYKILTAGTGPKPTAADTVVCQYKGTLLDGTEFDSSYKRGQPATFAVGKVI
                     KGWTEALQLMPVGSKWQLFIPADLAYGERGAGNVIPPNATLVFEVELVSIQAK"
     gene            complement(6184..6879)
                     /locus_tag="VN788_16095"
     CDS             complement(6184..6879)
                     /locus_tag="VN788_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HXU22105.1"
                     /translation="MKDSDIPGKPERSYLPAAGFHWLLPLYDLIIKLMGADRARSVLP
                     EQAELRPGQKVLDVGCGTGTLAILLKQRHPDIEVVGLDPDRKALALARRKAQRASVSP
                     HFDQGFSEALAYPADSFDHVFSSFMFHHLQNNQKEETLREIRRVLKPGGYLHLLDFCG
                     PESASGSLSLWFHSHHNLKDNSERRILTLIAKAGLADSRTVAYRTVLFGLAHVAYYQA
                     AALKLARAKTNQL"
     gene            7246..>8396
                     /gene="dinB"
                     /locus_tag="VN788_16100"
     CDS             7246..>8396
                     /gene="dinB"
                     /locus_tag="VN788_16100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HXU22106.1"
                     /translation="MPISSGSGAILRAMPKLAQYRAKHSAPETLPIESPPSILHIDMD
                     AFFVSVELLARPELKGLPVIVGGQRDQRGVVTSASYEARQFGVHSAMPLRTAGKLCPQ
                     AIFLDGHHELYGQWSDRVAAILAKFSPVVEMASIDEAYLDLTGTERLHGPPLGAAHKL
                     LREITAVTGLPCSGGLAATRLVAKVASEQAKPRGLVWVPPGSETSFLAPLSVRRIPGI
                     GRVTEAALKALGIETIAQLQALELAQLEDSFGRWGTALHRKARGIDSYEFFVDAEPKS
                     ISHNRTFGHDTNDRQQLLSTISHLCEKACKRMRDTGLHASNVTLTLRFADFTTITRSH
                     TLGEPSDLDAIFLSAIRELLSRAWDGVARLRLVGVSLASFSSGSQQIDLL"
BASE COUNT         1809 a         2383 c         2407 g         1797 t
ORIGIN      
        1 ttgcaactca atctggtcca tcgggatgtg tccggggccc tcgatcatca cttgcacgtc
       61 gtattcccag gcttttttgg tgagctcgcc cagcacgcgc aattcggaga attgcgcttc
      121 gtcgctcgcg tcggccaggc agccgggacg aaggccgtct ccaagcgaaa agctgacatc
      181 gtgcttctga aaaattttgc agatggcgtc gaaattttcg tagagaaaat tctgcgcctt
      241 gtggaagctc atccactgag cgagcaaagc gcctccgcga ctaacgattc ccgtgattcg
      301 gttgcggacc agcggaagaa attcgcgcag cacgccggcg tggatggtca tgtaatcgac
      361 gccctgctcg gcctgctcct cgatgacttc gagcatcacg ttgatcgtca gatcctcggc
      421 gcgccggacg cgcgaaaggg cttcgtaaat cggcacggtg ccgatgggca cgggcgaggc
      481 ctgaatgatc gcttttcgaa tcatcgcgat atcgccgccg gtggaaaggt ccatcaccgt
      541 atcggagccg taatgcacag cgcgatgaag tttttcgagc tcttcgctca cattcgaagt
      601 ggtggccgaa tttccaatgt tggcgttgat tttgcagccg aacgccaccc cgatgcccat
      661 cggctcgaga ttgcggtggt gaatgttggc aggaatgatg gcgcggccgc gcgcgacttc
      721 atcgcggacc gcgtccggcg agattttctc gcgcagcgcc acatactgca tttcttcggt
      781 gatgatgccc ttgcgcgcaa aatgcatctg cgagaaattc ctcgcgccgc cgttcatcgc
      841 ggcggaacga ttttcgatcc acgtatccct cagtgccata agtcctcact caaaaaaaag
      901 cgcgggcggc catgcgcgcc gcaacgcggc ttcattatcg cacccagact cccacccagc
      961 aactcgcgtc cgcgtacaaa ctgctcgcga tttttgcgcc cgctcgaaac gacggtgccg
     1021 ggcgttttcc agtgttgccc acttgctcac cgaagcgcag cgggtgtata gaggaggtgc
     1081 agaggaatca gatgagatgc atgggaccga aatttttgct cgcggcaatt ctgcttgccg
     1141 cgattccaag cgcggccttc gcgcatcacg gcacctcgca ctacgacctt ggaaaaacca
     1201 tcaccctgat gggaacggtc accggcttcg attggggcaa tcctcactgt ctggtttaca
     1261 ttgacgtgaa agccgataat gacaaagtcc agcactggac actggagctg gcgtctccct
     1321 acaccatgtc ccgcgcgggc tggaccagga attccttgat ggtgggagac atgatcctgg
     1381 cgcaaactca ccccgcaaga aacggaatta ctctgggcat cagcgcaact tcgagatcga
     1441 tcatgaagtt cgtcatcaat gggaaaccgc tccgaatgtg agctggacgc gcacgttgcc
     1501 caggttgtcg gccctgccca agatgtgcaa cccaacgctc agaacatgaa cttgagtgcg
     1561 gcctgcatca gccgcggcgg cgcggcgttg accacctggc cgaaaagcgc gctggaaaaa
     1621 tcgccattga cggctgctgg tccgaagaat tgcgtgtggt tgaagacgtt gaaagcttcc
     1681 agccgaaatt gcagtgtctt tgactccgat atctggaaag attttgacag ggcgaggtcc
     1741 gtgttgaata ggccggggcc atagaaaaat ctccgcctgg aatttccgaa cgtccccaac
     1801 gcattagggc caaataacga cgtattgaaa tatggcaggc cgttccgcgg atttccgtta
     1861 agtcgcagag gaccgccaac gaagtccggc aagtccgcgc tgtggttatt aactccgttc
     1921 gggaggcttc ccatcagtga gttgtccaaa tcggtggaga tggttacggg aaaaccagag
     1981 ctggcccgag tgattcccga gatagcccag ccttgcgtaa tcatgcttgc gcgagatgtc
     2041 aggcgatcga agggaagccg atactcgtag gtggcaacga agttgtgccg cagatcgaag
     2101 gccgagagtc ctcgcgttgc attgaagtta aaaggatcga gaatgtcggc caggctcgat
     2161 gcctggtcaa tcgacttgct gtacgtgtat ccgaacatta gatcgagagt ctttcccgac
     2221 cgccggagac tggcttgaaa cgagttgtag ttcgaactcg cgacactgcc ctcgtagtcg
     2281 tcattgctga acgctgatcc gagggggctt cgtgtgccgt ttatcgtggt gccacccgcg
     2341 gtaatgtacg tcgcatcctc tccacccggc ccgcacgtgg gcgatcccgg cgccacgtcg
     2401 ctcggttggc tcagcgacag acaaagcgcg gcattgccgg gatttgcgga aaataccgca
     2461 aggaggtggt gagcttgcga gccgacataa ttcaagctga gcaccgtgcc accgggcagt
     2521 tgacgttcga ctgaaaggaa gtagttttcg gcgtatggaa acgtgttgct tgggggcgga
     2581 gccgtcattc ccgcgatcgg ttcaaactgc gaaaaatcta tggtcggatt aggatggtgc
     2641 acagacgcat tcagcggggg gaacgtcaaa ggaaacggat tcacgtgcgc cgagccgtcc
     2701 gacgccgtga tgaatggtgt agcgaagagc gtgttgtttc ccgtgtagct taagccatac
     2761 ggaggctggg gttcgtcaaa cgcgatggta ttaccctgaa tcaccgaata aaagacgccg
     2821 tatccagtgc gaatacttgt ttttcctgcg ccgccgatga tcttgcccag aattccgttc
     2881 gactttgtag gcgagtaggc gaggccgatc cgcggcgcaa aacgattccg ctgtgggact
     2941 aacgtgctcg gaactccagc gtctgtcgcg tagacgatgc tggttggagc ggtcggataa
     3001 actttggact gctctcctag gataaacgtt ggaacctggt tttgtttttc ggaccagtac
     3061 tgcataagct cccaacggag cccgaaattt agcgtaagag tgggtttaat cctccagcta
     3121 tcctccgcaa acgccgccgg gtacttgtga cgcccccagt aatgctgcga atcggcttga
     3181 ttgtagttcg tcgaaactcc tatcaagaaa tctgcgaagt ccagaccggt ttccgtgccg
     3241 gagaatagaa acgatccatt gaaggtcggg ttcggattca cattaatctg ctcgacactc
     3301 agttcgaatc cagctttgat ggtgtggctt cctcggaccg tcgaaaaatt gtcggatacg
     3361 gtaaaggtgt tgttcgcctg cgctaggttt gtgatgggca ctccgacgac gaatgaattg
     3421 aagatcgtgt tttcaatccc ctcgatcgcg ggcgccagag caaaaatccc ggcgcacccc
     3481 ggagtcctgg tggacccggg gcaggtagta aatccttgtg actccagact tggacctatg
     3541 ccgcccacgg gctggcccac ggcgtttgcg ctgcgcatga agctgaagtg aaactcgttg
     3601 acattggcgc cactgaaagt cttcgtgtca gccaaattga ccagctgtga ccgccctgta
     3661 tttagggcgt tgaatcctgg gacgctcgcg ccgccctgcc cggttgggta gggattgttc
     3721 agcgtgaagt cgtcaaaaaa atagtaagcc gagagaagtc cccaccggtg gttgttcccg
     3781 tcaatgcgga aactcgcttt atcgtcccgt aataccttcc cctgtgacgc cgttgtaaaa
     3841 gtggcaggcc catcattcgg aggaggaatg aaattcaaga gggatttagc cggcgccgac
     3901 cacgctgctt gtggaatcat agcgcccggg aacacacaag tgctgctcgt acagcccgga
     3961 gtgaagtacg gctcgttgga gctcaccgga tagccaagct cgcccgtgag aataccggcc
     4021 agattcgcgc ctcccacggc cccgataagc gggctggggc cgatgccatt gggcccggtg
     4081 gtaaaatccc ccgaccggta ggcgagcgaa ggaacggaaa tcagccccgt gtcgatggcc
     4141 tgtgccatcc gtgttccttg atagtcagcg aagaagaaca ccttgccgct cttgatgggc
     4201 ccgcccaccg tgcctccgaa ctgattttgc cggaagaagg cgcgcgtagg agaaaagtaa
     4261 ttccgggcgt caagcacggt gtttcgcaga aattcgaaac cactgccatg tatctcattg
     4321 gtgccggact tcgtcaccac gttcacaatg ccgccgctgt aatcgccgta ttccgcgtcg
     4381 aagttattgg tcaatatccg gaactctgcg attgaatcga gatcgggcac gatcgtcgtt
     4441 ccaccgttca tcaattcttt gacgtcacct ccattgacca tgtagccgtt cgcatcctcg
     4501 cgctggccgc tgatggactg attgccggga ttcaaatcgc cagagggagc tatcgccacc
     4561 gtggcccctg ccatcacaat cgagttaggc tgctgagttg acatgggcac aatgcccggc
     4621 tgaagcgcca ggagatccgt gaaacttcgg ccgtttagag cgacacctgt gatctcagtg
     4681 cttgtcacca cttggccgac ctgcgtgctt tcggtctcca cctgggccgc agtacctgag
     4741 acgactacct cctgcgattg ctcgccaatt gtcagtacct gatcgacccg aagctgcgta
     4801 ttgacgtcaa taaccaagcc cgtgcgcttg tacggtttga acctgtcgcg aattatttcg
     4861 agttcgtagc gtcccaccgg aagatttgtg aaagcgtaaa acccttcgcc gttggtggta
     4921 gtcgtttgcg caacagcggt atccaggtta cgcaccgtga cagaggcgcc gggaatcacc
     4981 cctccgtttt cgtcggttac ggtccccgag atacttccgc ccgtcgtggc ccacacctgc
     5041 ttacagcaaa aaacaagaat gcctatgaat gctatccgga gcagtgtccg ggtggcgtgc
     5101 ttcatcgtta tctgtcctgt tgtagttgga gggctggcct gagctagaac cccgcaccca
     5161 caagaatcat aacctgaaaa gacgcattac acgcgccctt cgttaccgtc gcgcgcgcga
     5221 gcttttggaa gcgcttggct ggcgggaagt ttgtccacga ttgaaccatc atcagccacg
     5281 ccgatccgga tttgcaccgc cgcccgagca atgccacaat agcgggatgc gaaaattgtc
     5341 cctaacaacg ctaaccttaa tggcggctat cacgctgctg ctcggcagtt cctttgcgca
     5401 acagcctccc gctgcaaact cccaaccgtc tgctactgca aaaacgcaaa aaacgccggc
     5461 ggcaaagact cacgtggctc ctggaaccaa gaacagtact tccgccacta aaagcaacac
     5521 agggccggcc ctcaccacag acaaggaaaa ggatagctac gccctcggtt taaatatcgg
     5581 ccgaggcctc agcaagcagc ccgtggacct ggatgctgcg gccgttgcgc gcggtctcaa
     5641 ggacgccctg tccggcagca agtccctgct aaccgacgag gaagccgcgg cggacctcgc
     5701 gcaattgaag gatcaggcac agaaggcctc ggacgcaaag atgcaggagc tcggagccga
     5761 aaatcaaaag gcgggcgcag cattccttgc cgagaataag accaaagaag gtgtcgtaac
     5821 cctgccgagc ggccttcagt acaagatctt gaccgcaggc acgggtccga agcccactgc
     5881 agccgatacg gtggtttgcc aatacaaggg cacccttctg gacggcacgg aattcgacag
     5941 ttcgtacaag cgcggccagc ccgccacgtt tgcggtgggc aaggtgatta agggctggac
     6001 cgaggcgctc caactgatgc ccgtcggatc gaagtggcag cttttcattc cggcagacct
     6061 tgcgtacggc gagcgcggag cgggcaacgt gattccgcca aacgccactt tagtctttga
     6121 ggtcgagctt gtatcgatcc aagcgaagta agtagacagc ggtcttggag attcgatccc
     6181 tgctcacaat tgattagtct ttgctcgagc cagctttagc gcagccgctt ggtagtacgc
     6241 gacgtgcgcc agcccgaaga gcaccgttcg atacgcgacc gttcgcgaat ccgcaagccc
     6301 cgccttcgct atcagtgtga gaatccgcct ctccgagttg tccttcaggt tgtggtggga
     6361 gtgaaaccac aaggacagtg agccgctcgc tgattcgggt ccgcaaaaat ccagcagatg
     6421 cagataaccc ccaggtttga ggactctacg gatttcacgg agcgtctctt ccttctgatt
     6481 gttctgcagg tggtggaaca tgaaggaaga aaatacgtga tcgaaggaat cagccgggta
     6541 cgcgagcgcc tctgagaacc cttgatcgaa gtgaggtgag actgaggcgc gttgtgcttt
     6601 tcgcctcgcg agggcaaggg ccttccgatc gggatcgagc ccgacgacct caatgtccgg
     6661 atgccgctgc ttcagcagaa ttgccaaagt gccggtgcca caaccgacat caagaacctt
     6721 ttggccgggt cgcaactcgg cttgctctgg cagaacactg cgggcgcggt cggcgcccat
     6781 cagtttgatg atcagatcgt aaagcggtaa gagccaatgg aagcccgcag ctggaaggta
     6841 gctcctttca ggtttgcccg gtatgtccga atccttcata agttcattct ctcgccattt
     6901 taccaaattt agaaatgtgc tacgccgcgc catcgaaagt ccacaggttc tcgtcgtgac
     6961 gctttttcgc cgtgctgcta acttattctc gcaatgataa atgaggccta tcgagaagaa
     7021 cgctggcaac ggtacccgga agtgcgctcg aaggtgatcc gtttgcggac ccggcggtct
     7081 cgaaccggct gtgagggcaa tcacgcggaa cgcattccgc ggcggccgac cgctgcccgc
     7141 ccctaccttt ctaatcgacg ctacggtcga gtagaaattg gtctaagtga ttggaaacaa
     7201 acgatggcgg tctattctac tcgtcactct tttggagcat ggaaaatgcc catttcatct
     7261 ggatctggcg ctatattgag ggccatgccg aagctggccc aataccgcgc caagcacagt
     7321 gcgccggaaa cgctcccgat tgaatcgccg ccgtcgatcc tgcatatcga tatggacgcg
     7381 ttctttgtct ccgtcgaatt gctggcgcgt ccggagctaa agggcttgcc cgtgattgtg
     7441 ggaggccagc gcgaccagcg cggcgtggtc acttctgcca gctacgaggc gcgccagttc
     7501 ggcgtccatt ccgcgatgcc gctgcgcacg gccgggaaac tgtgtcctca ggccattttc
     7561 ctcgatgggc accacgagct ttacgggcag tggagcgacc gcgtcgcggc gatccttgcg
     7621 aaattttcgc cggtggtcga aatggcgtcg atcgacgagg cctatctgga cttgacgggc
     7681 accgagcggc tgcacggtcc tccgcttggc gccgcgcaca aactgctgcg ggaaattacc
     7741 gcggtcactg gactgccatg ctccggcgga ctcgcagcca ctcgcctggt ggccaaagtg
     7801 gcgagcgagc aagctaaacc gcggggactt gtttgggtac cgcccggcag cgaaacttca
     7861 ttcctcgctc cgctttcggt gcgacgcatt ccaggaattg gcagagtcac cgaggcggcg
     7921 ctaaaggcgc tcggtattga aaccatcgcg cagttgcaag ctttagagtt ggcgcagttg
     7981 gaggattcct tcgggcgatg gggcacggcg ctccatcgaa aagctcgtgg aatcgattcc
     8041 tatgaatttt tcgtcgatgc cgaaccgaag tcgatctcgc acaaccgaac ttttggtcac
     8101 gataccaatg acaggcagca attgctttca actatcagcc atctttgcga gaaggcgtgt
     8161 aagcgtatgc gcgacacggg gctgcacgcg tccaatgtta cgctgacctt gcggttcgct
     8221 gacttcacca caatcactcg aagccatacg cttggggaac cttccgatct cgacgcaatt
     8281 ttcctgagcg cgatccgcga acttttgtcg cgcgcgtggg acggggtagc gaggcttcgc
     8341 cttgttggcg tctcgctcgc ttccttttcg tccggctcgc agcagatcga tcttct
//
LOCUS       DATNDT010000491         4617 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3313196, whole genome shotgun sequence.
ACCESSION   DATNDT010000491 DATNDT010000000
VERSION     DATNDT010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4617)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4617)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4617
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3313196"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..388
                     /locus_tag="VN788_16105"
     CDS             <1..388
                     /locus_tag="VN788_16105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22107.1"
                     /translation="GAQQARHKIEGWKQGFRLGNKMILKFERDDGAEQTAESDGASAN
                     EESSSKKTAAKKRAAKEDNQDVAEPAGRVRLLVRLNFSDHEKLSHQRWLDRIPAEEPF
                     KSVKTETVRTGDPEFPAIAELFDSLD"
     gene            510..1004
                     /locus_tag="VN788_16110"
     CDS             510..1004
                     /locus_tag="VN788_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22108.1"
                     /translation="MSKTVMGFFDTYGEAADVVRDLELAGIVGEQVELVSAAEEEVPF
                     AEGSKKSSSAKRKAKAAAHDYYAEDPEFFAGESRGGRAAVIVRPVNSQVAETAAALFR
                     SHGAESPRGGSGPDVQNEDARPETSGSRSSAQKTPGIGANAATTGGAPHDLEARGQQP
                     HKKT"
     gene            1129..3297
                     /locus_tag="VN788_16115"
     CDS             1129..3297
                     /locus_tag="VN788_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HXU22109.1"
                     /translation="MPSEIKTVRFNASKYGGLDRDTLVRMYRTMFLSRRLDDREIQLK
                     RQNKTFFQISGAGHEAVLVAAGLLLKPTYDWFFPYYRDRALALMLGVSAYEILLQAVG
                     AKDDPSSGGRQMPSHWGNRRLNIVPGASATGMQFLHAVGSAEASLYYDKFPRALEQAR
                     QGSQGQSLTHESDEIAYVSGGDGSTSEGEFFEAVNAACLRHAPVLLLIEDNGYAISVP
                     VEAQTAGGSISKLVSNFPHLAVEECDGTDPLASYASLSRAVSHCRARLGPALVHAHVT
                     RPYSHSLSDDERLYKTSDEREAEAHRDPIPKFGLFLVREGVLDEREIGAIEAEVEREV
                     LDATDRALSAHPAEAASIYECVYSPDVDATGKDFEAVPNFAEHAAAKAVPKTMVEIIS
                     MTLADEMARDDRIVLFGEDVADCTREDYLNSVKGKGGVFKATMGLQRRFGSARVFNTP
                     IAEAAIVGRAIGMAVHGLKPVTEIQFFDYIWPAMMQLRNELSNVRWRSAGHFKAPVVV
                     RVAIGGYLTGGGIYHSQSGEVTFTHIPGLRVVMPSTALDLCGLLRTALRADDPVMVLE
                     HKHLYRQPYNRSPYPGADFTIPFGKARVAREGTDVSIITYGAVVHRSEVAAAELAREG
                     ISAEIIDLRSLSPYDWEAIAASVAKTNRAIVAYEDSRSWGYGAEIAARIADELFDELD
                     APVRRVAATDTFCAYHPGLEDAILPQSSDISRVVRDLAAY"
     gene            3477..3728
                     /locus_tag="VN788_16120"
     CDS             3477..3728
                     /locus_tag="VN788_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HXU22110.1"
                     /translation="MSKRLMRSSVDRKIGGVCGGIAEYFEIDSTVVRIIWVLLILMPV
                     PIVPAFLGYFVAWLVMPLAPWPVYQPEPPAAAPHTTQPA"
     gene            complement(3725..4180)
                     /locus_tag="VN788_16125"
     CDS             complement(3725..4180)
                     /locus_tag="VN788_16125"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXU22111.1"
                     /translation="MRSLEKLKPVALLLLRIALGIIFIYHGYPKLFGHTRETMVAFSD
                     MGFPRYFAYISGVVEFFGGCMLVLGLFARIAGLLLAGEMAIALWRVHGILSRPRAVGN
                     YEFPLLLAVSAFAVASLGAGLISFDHALFREGRHSSGKRRKMIKGLNPR"
     gene            4361..>4617
                     /locus_tag="VN788_16130"
     CDS             4361..>4617
                     /locus_tag="VN788_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HXU22112.1"
                     /translation="MRKLLVGLALVIFISAAAYFRFHHSKGPRETAYVGSRQVTLWST
                     SAQVREPTGIVNYGERLEILDRFQDQLHVRTTNGLTGWITQG"
BASE COUNT         1010 a         1240 c         1413 g          954 t
ORIGIN      
        1 aggcgctcag caggctcgcc ataaaatcga gggatggaag cagggctttc ggcttggcaa
       61 caaaatgata ttgaaattcg agcgcgacga cggggcagag caaacggcgg agtctgacgg
      121 cgcatctgcc aatgaagagt cctcaagtaa aaaaacagcc gcaaagaaac gagctgccaa
      181 ggaggacaac caagatgtgg cggagcctgc cggccgggtg cgcctgctgg tgcggctgaa
      241 tttctccgac cacgaaaaat tgtcgcacca gcgctggctc gatagaatcc cggcggaaga
      301 accgttcaaa tcggtgaaaa cggaaactgt ccgcaccggc gatccagaat ttccggccat
      361 tgccgagctt ttcgattcgc tcgactgagc ttgcgggttt gggctggccg gccttacgag
      421 tccccctaaa tacttcgctg tatcgacgtt tgccctcacc gctgctccaa tggttggagc
      481 ggccgttcca gccgcctgcg gagggcccca tgagcaaaac cgtgatggga tttttcgata
      541 cttatggcga agccgcggat gttgtccgcg acctcgaact ggcgggtatc gtcggcgagc
      601 aagtggaact tgtgagcgcg gcggaggaag aagtgccgtt tgccgaggga agcaagaaat
      661 ccagctccgc taagcgaaag gcgaaggcag cggcgcatga ttactatgcg gaggatccgg
      721 agttttttgc cggtgagtcc cggggcggcc gtgcggcggt gatcgtaagg ccagtgaact
      781 cccaagtggc ggaaacagcc gccgctcttt tccgaagcca tggggccgaa agcccgaggg
      841 gaggttccgg cccggacgtg caaaacgaag acgcgcgtcc ggagactagc ggctcgcgaa
      901 gttcggcaca gaaaacgccg ggaatcggcg cgaacgccgc cactacaggc ggagcgccgc
      961 acgatctcga agcgcggggc cagcaaccac acaagaaaac gtagcgcctt ccgagcttgg
     1021 taggctcgaa atgctcgcgg gctggttcga ggctctgcga cgcggctaat ttggctataa
     1081 tggttcgaac gtaacagctc acgaaactcc cgcgtcggag gcatcaggat gccctctgaa
     1141 atcaagacag tgcggtttaa cgcgtcaaaa tatggcggtc tcgatcggga tacgcttgtg
     1201 cgcatgtatc gcacgatgtt tctttcgagg cgcttggatg atcgcgaaat tcagttgaag
     1261 cggcagaaca aaactttttt tcagattagt ggcgcggggc acgaagcggt gttggtggcg
     1321 gcgggactgc tgctgaaacc cacttatgat tggtttttcc cctattaccg cgatcgagcg
     1381 ctggcgttga tgctgggtgt atcggcctac gaaatcttat tgcaggcagt gggggcgaag
     1441 gatgatccgt cgtccggtgg ccgccagatg ccgtcgcatt ggggcaaccg acgattgaat
     1501 atcgtcccag gagcttcggc tacgggcatg caatttcttc atgccgtcgg gtcggcggag
     1561 gcctctcttt actacgataa atttccgagg gcactcgagc aggcgcggca ggggtctcaa
     1621 ggacagtcgc taacccacga aagcgatgag attgcatatg tttcgggcgg agacggctca
     1681 acgagcgaag gcgaattttt cgaggcggtt aacgcggctt gcttgcgcca cgcacccgtc
     1741 ctgctcttga ttgaagataa cggctacgcc atctccgtgc ctgtagaggc gcagacggca
     1801 ggggggagca tctcgaagct tgtttcgaat tttccgcacc ttgccgtcga ggagtgcgac
     1861 ggaacggatc ctctggcaag ctatgcctcg cttagccgcg cagtcagcca ttgccgtgca
     1921 cggctcggcc ccgcgctggt tcacgcgcac gtgacgcgtc cttattcaca ctcgctttcc
     1981 gacgatgaga ggctctacaa aacctccgat gagcgagagg cggaagcgca ccgcgatccg
     2041 ataccgaagt tcggactttt cctggtgcgg gagggcgtgc tggatgaacg cgaaattggc
     2101 gcgatagaag ccgaagtgga gcgcgaagtt ttggatgcta ccgatcgcgc gctttccgcc
     2161 catccggcag aggcggcatc catttacgag tgcgtttatt cgccggatgt ggatgcgacg
     2221 gggaaggatt ttgaggctgt gccgaatttt gcagaacacg ctgccgcaaa agccgtgccg
     2281 aaaaccatgg tcgaaattat ttccatgacg ctagcggatg aaatggcgcg cgacgatcgg
     2341 atcgtcctct tcggcgaaga cgtagcggat tgcacgcgcg aggattacct gaatagtgtt
     2401 aaaggaaaag gcggagtctt caaggcgact atgggattgc agcgtcgatt cggttcggcc
     2461 cgcgttttca atacgccaat tgccgaagct gccatcgtag ggcgggccat tgggatggcg
     2521 gtccacggat tgaagccggt gacggaaatt cagtttttcg actacatctg gcccgcgatg
     2581 atgcagcttc gcaacgagct ctcgaatgta cgttggcggt cggccggaca cttcaaagcc
     2641 ccggtggtgg ttcgggtggc gattggcggc tatctgacgg gtggcggaat ttatcacagc
     2701 cagagcggtg aagtgacgtt cactcacatt ccgggtttgc gcgtggtgat gccttcaacg
     2761 gccctggatc tttgcgggct tttgcgcacg gcgctacggg cggacgatcc ggtgatggtg
     2821 ctcgagcaca agcatctcta ccggcagccg tacaatcgat cgccgtatcc gggcgcggat
     2881 ttcactattc cattcggaaa ggcgcgcgtc gcccgcgaag gaacggatgt aagcatcatc
     2941 acttatggcg ccgtggttca tcgctccgaa gtggcggcgg cggaactggc acgcgaaggc
     3001 atatcggccg aaattatcga tctacggtcg ctcagccctt atgattggga agcgattgcg
     3061 gcaagtgtgg ccaagaccaa ccgagcgatt gtcgcctacg aggattctcg ctcgtggggt
     3121 tacggggccg aaatcgcggc ccgcattgca gacgaactat tcgacgaatt ggacgctccg
     3181 gttcgccgcg tcgccgctac ggacacgttt tgcgcatacc accccggact cgaagacgcg
     3241 atcctgcccc aaagcagtga catctcgcgg gttgtgcggg atcttgcggc atattgatat
     3301 gccggtgaaa ctttccgggg atccaagccg tacttgaata tgtgcgggcg aatctcggga
     3361 gcgaggaggt ctgacttatg cattgcggaa attgccaaag agagatcgcg gaatactcga
     3421 atttctgtta cttctgcggc gcacggcagc gtgcgcggta cgcggaacct ccgggcatgt
     3481 ccaaacgtct gatgcggtct tccgttgacc gcaagattgg cggggtctgc ggcgggatcg
     3541 ccgaatattt cgaaattgat tcgacggtgg tgcggattat ctgggtgctg ctgatattga
     3601 tgcccgtgcc gatcgtcccg gcattccttg gatatttcgt ggcctggctg gtgatgcctc
     3661 ttgccccgtg gcccgtctat cagcccgaac ctccggcggc ggcgccgcat accactcaac
     3721 ctgcttagcg gggattgagt cccttgatca tttttcttcg ttttcctgaa gaatgacggc
     3781 cctcgcgaaa aagtgcgtgg tcgaatgaaa tcagccccgc cccgagcgat gcaacggcga
     3841 acgcgctgac agccagcaaa agcggaaact cgtaattgcc aaccgcccga ggtcgcgaaa
     3901 gaatgccgtg aaccctccat agcgcaatag ccatttcacc cgccaacaat aagcccgcga
     3961 tccgcgcgaa aagcccgaga acgagcatac atccgccgaa aaattcaaca acgcccgaaa
     4021 tatacgcaaa atatcgcgga aaccccatgt cagaaaaagc caccatggtt tcacgagtgt
     4081 gtccgaatag tttcggatat ccgtggtaga tgaaaataat tccaagagcg atccgcagaa
     4141 gcaaaagggc gacaggcttc agcttttcga ggctccgcat caatactccc ttctagattg
     4201 tttggggcac tgcagctacg actcggccgg attgtagcag agtcgcccgg gcccattttc
     4261 gacacatcgg gattttttcc tagaaccaat gggaagccgc gccttccacg ggtgtagaat
     4321 ccctcgttac cttgaaaatt gctattgagt ggggccgtcg atgcgaaaat tactagttgg
     4381 tcttgcgttg gtaattttca tatccgcggc cgcatatttt cgttttcatc actccaaggg
     4441 gccgcgagaa acggcctatg ttggcagccg ccaagtgacg ctgtggagca caagcgctca
     4501 ggtacgcgag ccgacaggga ttgtgaatta cggagaacgc ctggaaatac tggatcgatt
     4561 tcaagatcag ctgcacgtcc gcaccacaaa cggtttgacc ggctggatca ctcaggg
//
LOCUS       DATNDT010000492         4445 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3761872, whole genome shotgun sequence.
ACCESSION   DATNDT010000492 DATNDT010000000
VERSION     DATNDT010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4445
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3761872"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1274
                     /locus_tag="VN788_16135"
     CDS             <1..1274
                     /locus_tag="VN788_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007511125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU22113.1"
                     /translation="SDQTIVIRDGRISVLGDAATTKVPDGAKVLDLSGRTVIPGLVGM
                     HDHMFYPAPGRSIPLYNEHAASFPRLYLAAGVTSLRTTGSLEPYTDLELKRAIDEGKM
                     AGPKIHVTGPYLEGQGAFTLQMHQLKDAEDARRTVEFWISEGVTSFKAYMNITPKELG
                     ASIKAAHAHGIKVTGHLCSIGFREAAALGIDDLEHGLLEDTEFLPAKKPGLCPPGDAA
                     EKVLLGMDVTSGPIHEMILDLVAHHIAVTSTLPVYETFVPGRAPLDARVLDAMLPESR
                     IYYLTRRARISDGAAKSDWPALFKKEMEFEREFAKQGGRLLAGLDPTGYGGVIAGFGD
                     QHEVELLVEAGFTPLEAIHIATSNGAEFIGELDRIGTLAAGKQADIVVINGDPSQNIK
                     DIEKVEIVFKDGVAYDSPKLIQSVQGFVGLH"
     gene            1388..2572
                     /locus_tag="VN788_16140"
     CDS             1388..2572
                     /locus_tag="VN788_16140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020564474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain"
                     /protein_id="HXU22114.1"
                     /translation="MRVLISGAGIAGSTLAYWLARYGMQPTLVEKAPRLRTGGYVIDF
                     WGAGFEIADRMGLLPEITLKGYKVQELRVVNRAGKTISRFPFAAFDRLTHGRFITLPR
                     SELAASIFGLLKGNVETIFGDSVSRIDQSDEGAHVAFESGMERDFDLVVGADGLHSRV
                     REIVFGPENRFERYLGCKVAAFEVAGYKPRDELIYVLYTKVHQQVGRFTMRGDRTLFL
                     FTFADEQAAGPCDLASQKSLLRKRFGNSGWECPQILDALDSANDFYFDRISQIRMDEQ
                     EGLWTRGRVTLVGDAACCVSLLAGQGTALAMVAAYTLAGELHRVNRNYAQAFARHQAL
                     FGPFVREKQKGALRFADFFAPKSKSSLFLRNQIMRLLDFPWIADLAIGRDIADKIPLP
                     EY"
     gene            2982..>4445
                     /gene="zwf"
                     /locus_tag="VN788_16145"
     CDS             2982..>4445
                     /gene="zwf"
                     /locus_tag="VN788_16145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HXU22115.1"
                     /translation="MVIFGAGGDLTKRMLIPAMYNLCKGKLLPDEFAIIGVSIETFSV
                     DEFRERASKDIREFAVGDVDMKSWDWLVKRLYYVSGDFHDAKMYGKLKETLDQVKKEQ
                     STGGNVLFYLATSPEFFPVVVQQLGAAELTKQDAHTWKRVVIEKPFGHDLPSAIALNN
                     AVGEVLDEKQVYRIDHYLGKETVQNILAFRFANGIFEPVWNRRYVDHVQVTVAEELGV
                     ELRGGYYETAGALRDMVPNHIFQLITLCSMEPPISFDADAVHDEQTKILRALQPFSPE
                     KVLDHAVRGQYGEGTIEGKRVPAYRNEPHVAPDSQTPTFVAMALHIDNWRWAGVPFYL
                     RTGKRMAARATQIVIRFRRPPLVLFRKTSIDRITPNELVINIQPEEGISLSFEPKIPG
                     PVVRLGAVNMDFQYSDYFGTTPSTGYETLIYDCMMGDPTLFQRADMVEAAWAVVQPIL
                     DVWKALPPRDFPNYAAGTWGPSQADELLRREGREWRDP"
BASE COUNT          965 a         1318 c         1193 g          969 t
ORIGIN      
        1 gttccgatca aacaatcgtc attcgcgatg gaagaattag cgtcctcgga gacgccgcaa
       61 ccacaaaggt gcccgacggc gccaaagttc tcgatctcag cggacgtacc gttattccgg
      121 gactcgtcgg tatgcacgac cacatgtttt atcctgcgcc cggtcgttcc atacctctct
      181 acaacgagca cgcggcaagt tttccgcgcc tgtatctggc ggcaggagtc acgtcactgc
      241 gcaccaccgg cagccttgag ccttacactg acctcgaact gaagcgcgcg atcgacgaag
      301 gcaaaatggc gggacccaag attcacgtca cggggccgta tctcgagggt cagggcgcat
      361 tcactctcca aatgcatcag ctgaaagacg ccgaagatgc acgccgcacc gtcgaatttt
      421 ggatctccga aggcgtcacc tccttcaagg cctatatgaa catcaccccc aaagaactag
      481 gagcgtccat aaaggctgct catgctcacg gaattaaagt cactggacat ctttgctcca
      541 tcggttttcg cgaagccgcg gccctgggca tcgacgacct cgaacacggc ctgctcgagg
      601 acactgagtt tctcccagcc aagaagcccg gcctgtgccc ccctggcgat gccgctgaga
      661 aagttctgct cggaatggat gtgaccagcg ggccaatcca cgaaatgatt ctggatttag
      721 ttgctcatca cattgccgtc acttccacgc tgccggttta tgaaactttc gttcccggcc
      781 gcgcccctct ggatgcccgc gttctggacg ctatgcttcc cgaatctcgg atctactacc
      841 tcacgcgtcg cgcccggatt tctgacggcg cggcaaaatc cgactggccc gctcttttca
      901 agaaagagat ggaattcgag cgtgagtttg cgaagcaagg cggccggctc cttgccggac
      961 ttgatcccac cggatacggc ggcgtgatcg ccggatttgg cgaccagcac gaggtcgagt
     1021 tgctcgtcga agcggggttc acgccgctcg aagccattca cattgccaca tcgaatggcg
     1081 ccgaatttat tggcgagttg gatagaattg gcacgctggc cgccggcaaa caggccgaca
     1141 tcgtcgtcat caacggcgat ccctcacaaa atataaaaga catcgaaaag gtggaaatcg
     1201 tgttcaagga cggcgtggct tacgactccc ccaaactgat ccaatccgtt caagggttcg
     1261 tcggcctcca ctgacgaatg cgcacaaccc cgacttcggc gctgtatagg ccgcagccga
     1321 ttcggccgcg cctttttttc cacagtcacg tcatcgcaaa atgtcggggt ggtattctag
     1381 ttgtgtcatg cgggttttga tatctggcgc gggaattgct gggtccactc tcgcatactg
     1441 gctggcccgc tatggcatgc agcctactct cgtcgagaaa gctccccgat tgcgtaccgg
     1501 cggttacgtt attgattttt ggggcgcagg tttcgaaatc gcggatcgca tggggctgct
     1561 gcccgaaatc acactcaaag gatacaaggt ccaggagctg cgagttgtaa atcgagccgg
     1621 aaaaacaata tcccgatttc cgttcgctgc gttcgaccgt ctaactcacg gtcgcttcat
     1681 caccctcccc cgaagcgagt tggccgcgtc gatctttggc cttctcaaag gtaacgtcga
     1741 gacgatcttt ggcgatagcg tgtcccgaat cgatcagtcc gatgaaggcg cccacgtcgc
     1801 ctttgaaagc ggaatggaac gcgacttcga tctcgtcgtg ggcgccgacg gccttcattc
     1861 gcgagtccgc gagatcgttt ttggcccgga aaaccgcttc gagaggtatc tgggatgcaa
     1921 agttgccgcg ttcgaagtcg ccggctacaa gccgagagac gaattaatct acgtgctcta
     1981 tacaaaagtg caccagcagg taggtcgctt taccatgcgc ggcgaccgca cgttgttcct
     2041 gtttacgttt gctgatgaac aagctgctgg cccctgcgat ctcgcatctc aaaaatcact
     2101 tttgcgaaaa cgcttcggaa atagcggatg ggagtgcccg cagattctgg atgccctcga
     2161 ctccgcgaac gacttctatt tcgatcgtat cagccagata cgcatggacg agcaagaagg
     2221 cctatggacc cggggacgcg taactctggt cggggatgca gcttgctgcg tgtcgctgct
     2281 cgcaggacaa ggtaccgcct tggccatggt ggcagcctat accttggcgg gagagcttca
     2341 ccgcgtaaat cgcaactatg cccaggcgtt tgcccgccac caagccctgt tcggaccatt
     2401 tgttcgcgaa aaacagaagg gcgcgctgcg ttttgccgat tttttcgcgc ccaagtcaaa
     2461 gtcctctcta ttcctccgca atcagatcat gaggctactg gacttccctt ggattgccga
     2521 tcttgctatc ggccgtgaca tcgcagacaa gatcccactt ccagaatact gatccggcca
     2581 cctggcccac ataagaaagt ccacagactc tcgcccttgc gctccccctc tcgtgtatta
     2641 agatggatgc gtgaaaactt ttgtagaacg tgacgggccg ttcgcaggtc tcgcgaacac
     2701 tgacgcctcg cgagagccct cgccgcacct ccgtgcgcgt ttccgtggct ctcgtcccca
     2761 aggtcggcag cgaattccca tcgagcggcc ccgcgaatct aatcgacaca tcgtacgagt
     2821 agaaagcctc ctaagttatt gcaaacaaag gatgggcatc gcatctaccc gtcacgcata
     2881 tcggggcctt ttgcagcccc tcggaggttc gcctcgtggc tccaactgat tctgggaagc
     2941 taacgcaaga gaccttgggc agccctgcag ggccctgcgc gatggtcatt ttcggtgccg
     3001 gcggcgacct caccaagcgc atgctgatcc cggccatgta caacctttgt aaaggcaaat
     3061 tgctccccga cgaatttgcc atcatcggcg tctcgatcga aaccttctcg gtggacgaat
     3121 tccgcgagcg agccagcaaa gacattcggg agtttgccgt cggcgacgtg gacatgaaat
     3181 cttgggattg gctcgtcaag cgcctttact acgtgtccgg agattttcac gacgccaaaa
     3241 tgtatggaaa attaaaagag accttggatc aggtgaaaaa ggagcaatcc accggaggca
     3301 acgtcctctt ctacctggcc accagtcccg aatttttccc cgtggttgtg cagcaactcg
     3361 gcgcggccga gcttacgaaa caggatgctc acacgtggaa acgtgtcgta atcgaaaagc
     3421 ctttcggcca tgatttgccc tccgccatag ctctgaacaa cgccgtcggc gaagtgctcg
     3481 atgaaaaaca ggtctaccgg atcgatcact accttggaaa agaaactgtt caaaacatct
     3541 tggccttccg gttcgccaac ggaatcttcg agccggtgtg gaatcgccgt tacgtcgacc
     3601 atgtacaagt caccgtcgcg gaagagctgg gcgtcgaact acgcggcgga tattacgaaa
     3661 cggcaggcgc tctgcgggac atggtcccca atcatatttt tcagttgata actctctgtt
     3721 cgatggagcc cccgatttct ttcgatgccg atgcggtcca cgacgaacaa accaaaattc
     3781 tgcgagccct tcagcctttc agccccgaaa aagtgctcga ccatgccgtg cgcggccaat
     3841 atggcgaagg cacgatcgaa ggcaagcgcg tgcccgccta tcgcaacgag ccgcacgtgg
     3901 cacccgattc gcagactccc acatttgtgg ccatggctct gcacatcgat aattggcgtt
     3961 gggctggagt tcccttctat cttcgcaccg gcaaacgcat ggccgcgcgc gccacgcaga
     4021 tcgtgattcg cttccgcaga cctccgctcg ttctttttcg caaaacctcg atcgaccgga
     4081 ttacgccgaa tgaactagta atcaatattc agcccgagga aggaatttcg ctgagttttg
     4141 aacccaagat tccgggccct gtcgtccgtt tgggtgcggt gaatatggat tttcaatatt
     4201 cggactactt cggcacaacg ccgtccaccg gatacgaaac actcatctac gattgcatga
     4261 tgggcgatcc caccttattc cagcgcgcgg atatggtgga ggctgcctgg gccgtcgtgc
     4321 agccgattct cgatgtgtgg aaggctttgc cgccgcgcga ttttcccaat tacgctgcgg
     4381 gcacttgggg gccttcgcag gcggacgaac tgcttcggcg cgaagggcgc gagtggaggg
     4441 atcca
//
LOCUS       DATNDT010000493        13526 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_11135, whole genome shotgun sequence.
ACCESSION   DATNDT010000493 DATNDT010000000
VERSION     DATNDT010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 13526)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13526)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13526
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11135"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..215)
                     /locus_tag="VN788_16150"
     CDS             complement(<1..215)
                     /locus_tag="VN788_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22116.1"
                     /translation="MQEDERDLLEVLKDELKFIESGGYARSPGASWRPVFIFEDSPAC
                     MNHDHTNDPESCSDCVLMQLVPPELRSA"
     gene            550..1518
                     /locus_tag="VN788_16155"
     CDS             550..1518
                     /locus_tag="VN788_16155"
                     /inference="COORDINATES: protein motif:HMM:TIGR03508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DmsE family decaheme c-type cytochrome"
                     /protein_id="HXU22117.1"
                     /translation="MRAFALSKVSGLLITLGLLSAFVLAQPPETLASADSHGAAIQEK
                     SASSNPETKPSDYAGAEACKTCHEEEYKSWENSPHWKTTLNTKEGPSHQGCEGCHGAA
                     ASHVADPSDTSKLFLFENASAKDINQRCLVCHASGTQHMQAIDSLHAKNDVSCTSCHS
                     PHHAKQAEFLLVKSQPELCYSCHLQQKAQFAMPFHHRVNEGLLQCTDCHNPHGTAGPH
                     QLRTSAAQDAVCYKCHADKQGPFVFEHEVVKVEGCESCHNPHGSPNPHLVKLSNVNLL
                     CLQCHTTSFANAPGAPSFHNQAAQFQACTLCHTQIHGSNFDKDFFK"
     gene            1601..4114
                     /locus_tag="VN788_16160"
     CDS             1601..4114
                     /locus_tag="VN788_16160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22118.1"
                     /translation="MKLRVRESALGWTALIVALLFAFPVRAQQAQQAPPQTSQQTDST
                     SSDEGMKSGDYNVRQSIEFGYRDSSIDGNIGNYDTFENLGSGFRLFDYTLDMRSVDHK
                     GFFFDSLTFSNFGYGGDPNNVTRLRVEKNKLYDFRAMFRRDKNFWNYNLLANPLNPAS
                     SVPALAITSSPHSLDLVRRMGDYDLTLLPESRLRFRLGYSRNVNEGPGFTTLDSGTEP
                     MLTENFRYTTNAYRLGVDYRFLPKTTISFDELLNYFKQDSTVTDQNFPFVLSNGTPAD
                     LGIIFNTKGNTPCATPVTSAATTPPTANANCNGFLAYSHVGRPRGSFPTERLSFQSSH
                     FKNLEMSGSFGYSSDSNSTLDFNEVVNGWTSRTVTRGSTVAGPANAKRVSVNADWAGD
                     YRVTDRLHIVDEFRYDNWRIPSMWATAETNLFATPPAAPGQTGLLLPMAQVNPANFAT
                     VCPAAPYNGPNCPQHVSGSLADVTNEFVSRFLGQNLKTNTIEVQYEIAPRIDAHIGYV
                     YTDRTISDFNATFDTGEIYFPGGVTGNAGNDFLAARGDCALVGGALPAVCTMNPDGSI
                     FEGSPTNPVPEAGNDTSRGITDIHENALLVGISARPTDTFRIGADFMFGYNDTSFTRI
                     SPRQIQSYKIHATYTPKQWATIIGAIDIHENRDNVLAVNNLEHGRTYSFGATLAPSSR
                     LWVDFGYSYMDISIQTDICFAEPGSTVFTTPCPVAGATGPLGALSFYTNTDHFAYGHM
                     MWKPVKRVTAALGYSGTIAQGNTLFLNALQPTGPLDFNYLKPSASLTIDVYRGLSYKT
                     AWNYYGYNDHGVANPIGLAQLPLQDFNGNNITFSVRYLF"
     gene            4177..4515
                     /locus_tag="VN788_16165"
     CDS             4177..4515
                     /locus_tag="VN788_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXU22119.1"
                     /translation="MNLSLRIGKALFLAVAFLFAFSAVTRADDASSLYKTKCAVCHAP
                     DGSGSSTMGKKLGAKDLRSDEVQKKTDADLNGVITNGMGKTMPAYKGKLTDDEIKQLV
                     GFIRDLGKKK"
     gene            4574..5173
                     /locus_tag="VN788_16170"
     CDS             4574..5173
                     /locus_tag="VN788_16170"
                     /inference="COORDINATES: protein motif:HMM:NF015237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HXU22120.1"
                     /translation="MLIAQIILSIVLACIFLIRPSVTKATGWKIVAFIGLCVLPALCI
                     VGGMNIHMQRSEQTRFCISCHAMAPYARSLYVDDPSYIPAQHFQNHRVPADMACYACH
                     ADYSIYGPLKDKMRGLTRIYMQYVSTPPATIRIPGGYDNNQCLHCHAGARDFEGNAIH
                     SAAMDALKSNQMSCISSGCHDTVHNVTALDHVKFWRPGQ"
     gene            5170..5388
                     /locus_tag="VN788_16175"
     CDS             5170..5388
                     /locus_tag="VN788_16175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22121.1"
                     /translation="MTFRNLEFKWLLRISSALIILGLLVEIASLLWFHPLSFVLFTFV
                     GASLIGLGIVVYLASLVFVASSGGERHE"
     gene            complement(5945..6442)
                     /locus_tag="VN788_16180"
     CDS             complement(5945..6442)
                     /locus_tag="VN788_16180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22122.1"
                     /translation="MSKAREIRCYDYVNHPYERVRDALKRDALAVFQSATKAAASRAQ
                     SIAAELHVDVGGIGVKTDIKISVKSVEEQVHGGVANPTTRLLLEWEAAAMPRLFPLMR
                     AELSVYPLTATETQLDFSGLYEPPFGALGKAMNAMVGHRIAEVSVHRFIGNVAEYLRQ
                     TLAYK"
     gene            complement(6519..7874)
                     /locus_tag="VN788_16185"
     CDS             complement(6519..7874)
                     /locus_tag="VN788_16185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22123.1"
                     /translation="MKLRCGVHNARTALLIVAALLLTCGATFAQVSPAEILNPRLKAA
                     ETKYLPQLESLHQAITATKFPFSFALARYVQSEPSKQSSFDSRGIEFVYFQNRVVLKI
                     SGIYKAAYNADQLTQNQRASRIFRDVIAPILWLVAKQIPRDVDCDAIGFEIVYDAHTS
                     SKNYDYEGKEILAAVLDRADAFALVSAPEREQQDILNRSRIYVNGKDFGLALDQLDPF
                     DVEALARSIPARTVVPSSSAAGAISSSVTNPPAASPAPAAAVAVKMPRATPASVTAPP
                     TVFQPIAASPSVTASRPGSTQADAERLQSQFQSRLDELVKTNGAEFHFVDYAQPSLVV
                     YHNRIVLQFTLRDPTQFQSSSTSIYKRAAQSFDLFLAPRLKALMQKLPSSDELEAIDF
                     SVLHHLGGEKESSEAAEFICPVKALRSFTADEITSQELINQSIVLVNGVRITLNLQAV
                     E"
     gene            complement(7888..8364)
                     /locus_tag="VN788_16190"
     CDS             complement(7888..8364)
                     /locus_tag="VN788_16190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HXU22124.1"
                     /translation="MRAGALLFSAALGLAVLAGCARKSVPVKSDPLQNAYDTEPDWND
                     AQRIIPLSYEAAQGKQIFYQQCVWCHADQTPAGPSNRSNVTPAPPLWNDGATLNSLSD
                     TFLEKFTAQGGTAMGKSAMMPPYGQTLTPEEIRAVVAYARAIAAPAPQGPSRPRQK"
     gene            complement(8361..10493)
                     /locus_tag="VN788_16195"
     CDS             complement(8361..10493)
                     /locus_tag="VN788_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HXU22125.1"
                     /translation="MNRRSAQGLVGWVRRVFFGDPARRFAWVSLLLTLSLAVAPTKDF
                     FRQWRHYQRQYFKFIEGRPNAAALRGRTVSGIYQIWLPDLGVVDRCTTCHLGIAEPSL
                     AGASVPEPFRAHPPIPHGTEAWGCVVCHRGQGSATEVAEAHETTVAWEQPILPIHFIE
                     ASCGTCHRDDLPQAPQLDQGRQLLARFDCTGCHKIAGIDRPAMLGPDLTDIGTKVSRE
                     WLYKWLKEPRTITDANGSVTVDGVDFGEPQMPTFRLSDPELRDLSAYLSTRNPHPIQP
                     YHFDPRIVASLSKRPDVADQGEARFRQMFCSSCHSLAVTRVGETSLIGGDIGPELTKI
                     GSKVNPDWLAAWLRNPQDYLPHSQMPRYEWSDADLYEVTHYMETKLTDPDLLADVPKL
                     GSPTKTEIDAGRRLFVDKGCASCHVTEGLRPQEDFGPDLSTIGARNISQLDFGAAKIP
                     HTLISYIQAKISSPISVNPAGRMPQYHLDQASLDAVTTALLSLTGPPATRGMEKLIVA
                     RQKPEFHPAGEFGKLYERYKCYACHKFNGFGGTLAPDLSYEGSRATRQWLLQFMENPQ
                     TLRPTLVLRMPQFNMSERDAAVFADYFETVMQSPAVDALDRKEFTPAMAAAGKQLYEK
                     KYECQSCHTIGSAGGYVGPSLGNAGNWLTPGWIEAWLRDPQALVPGTIEPKQAFIADE
                     IQALTAYLLTLKQSAPAGGAAASTRGTK"
     gene            complement(10490..11131)
                     /locus_tag="VN788_16200"
     CDS             complement(10490..11131)
                     /locus_tag="VN788_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22126.1"
                     /translation="MTSDPSAPREATLPRRIDEERSIPYWEMLFRETIAIELLASVLV
                     WIALWWNAPLELLADPQQTPKLAKAPWYFTGLQELLHYFPPFVAGIILPTLIVVALIV
                     VPFFNVNIKDENLWVHHKSQRLAILGIILAALTVLLIRFDAWDALLPVWLIAIFMGLA
                     AANEGRRDRGFRRWLSSKPLSFWIMTWFLMEAVVLTSVGTFFRGPGWAWIWPW"
     gene            complement(11128..11733)
                     /locus_tag="VN788_16205"
     CDS             complement(11128..11733)
                     /locus_tag="VN788_16205"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HXU22127.1"
                     /translation="MRERQLGFRYTWYLGALTLGTFSIQLITGLLLMLYYHPSIPQGY
                     ADMKDLHFVVSSGVFLRNLHRWSAHAMVFLVFAHMAKVFYRGAYRPPRELNWVVGVCL
                     LLLTLLASYTGYLLPWDQLSYWGTTVGSSIISSTPFIGERLRFILLGGHTVNANALLR
                     FYVLHVVILPLAVILLVAIHLWRLHKDGGMYIEHEKDVEKP"
     gene            complement(11838..12341)
                     /locus_tag="VN788_16210"
     CDS             complement(11838..12341)
                     /locus_tag="VN788_16210"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HXU22128.1"
                     /translation="MSEPENKNLAASNDEESFLSRRQFFVKLGAGSLAVAGAGATVFA
                     VQYLSPNVLYEPSPIVGVGRPEHFPIDSVTLDPRTAIFVVHAQKGFYAMSSVCTHLGC
                     LTVWRQDEGIIACPCHGSTFRPDGKVMGGPAPRPLQWLKMWIGNDGDLIVDRSTNIAS
                     ESEYVQL"
     gene            complement(12364..12975)
                     /locus_tag="VN788_16215"
     CDS             complement(12364..12975)
                     /locus_tag="VN788_16215"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU22129.1"
                     /translation="MKHVIRAVVLAAICFVPLLFNGVARAGDIKGTVSVQGLRSAQDI
                     AVYVDAIPGKHFDAPAQHIVVDQRKMTFVPHVVVVLQGTTVDFLNSDPVGHNVYWPSI
                     SGNKKLAHNLGTWPKGERKSFQFNDLGAASLLCNVHPEMSGYVVVVATPYFAVADKDG
                     SFEIKNVPPGTYTLKTWSEEGKPTTQSITVGSGALNVDLTVKK"
     gene            complement(13030..>13526)
                     /locus_tag="VN788_16220"
     CDS             complement(13030..>13526)
                     /locus_tag="VN788_16220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22130.1"
                     /translation="TLGAFGYYGRNELNVDGALFPGLSTIREPFYRVGADFRFKYRQF
                     ELWGLGMYGHDTNEALNSGGTGFVSATPVTFSGGFAEAEYWVYPWLIGLMRYDVVNSP
                     TDFLNGVSRSATRNRFSPGVQVLVRANIKLAFEYQRRFEEPVPGTITFFRPNGFVVGT
                     DFVF"
BASE COUNT         3075 a         3786 c         3729 g         2936 t
ORIGIN      
        1 gcggagcgaa gctcgggtgg cacaagctgc atgagtacac agtcgctgca cgattccgga
       61 tcattcgtgt ggtcgtgatt catgcacgcg ggagaatctt cgaaaatgaa aactggtctc
      121 cacgatgcgc caggcgaacg cgcatatccg ccactctcta tgaatttgag ctcgtctttg
      181 agaacttcca gaagatctct ctcgtcttct tgcattttct gccacttcga caccgccttt
      241 aactatgcgc attcgggaat atcagtgctg tgactggcgt cacattgagt agtaactgct
      301 gtcacttccg aaatatgagg attcgaagca gactagccgc tggaagaggg ggacggtaag
      361 ggtacactgc gatgaccagt tccgttatgg gacacgggca ccctgcaact taggaaaaga
      421 gttgaatttg tgcggtattg ctcagacacc cgaaagggtg aactgtactc tctacgggac
      481 cgaagtttat ttactgcagc ccgctttaag gcggtatgcg tgatgtcggc ggcgattggg
      541 ggaagttcga tgcgagcttt cgcgctttcg aaagtttcgg gcttgctcat cacgctaggt
      601 ctgctgtcgg ctttcgtcct ggcacagccg ccagagacgt tggcgagcgc cgattcgcat
      661 ggggcggcca tccaagagaa gagcgcgtcc agcaatcctg aaacaaaacc ctctgattac
      721 gccggtgcag aagcttgcaa gacctgccat gaagaagagt acaaatcctg ggagaattcc
      781 ccgcactgga agacaacgct gaacaccaag gaaggccctt ctcaccaggg ttgcgagggg
      841 tgtcacggcg cggcagcaag ccacgttgcg gaccccagcg atacctcgaa gctctttctg
      901 ttcgaaaacg catcggcaaa agacatcaac cagcgctgtc tggtctgcca cgcttccggc
      961 actcaacaca tgcaggcaat cgactctttg catgccaaga atgatgtgtc ctgcacttcg
     1021 tgccattctc ctcaccacgc gaaacaggcg gagttcttgc tggtcaaatc gcagcccgag
     1081 ctttgttatt cctgccactt gcagcagaag gcgcaatttg cgatgccgtt tcaccatcgc
     1141 gtgaatgaag gattactgca gtgcaccgac tgtcacaatc cgcatggaac ggccgggccg
     1201 caccagcttc gcacttcggc ggcgcaagat gcggtttgct ataagtgtca tgcggacaag
     1261 caaggcccgt tcgtcttcga gcatgaggtt gtaaaagtgg aaggctgcga gtcgtgccac
     1321 aatccgcacg gttccccgaa tcctcacctg gtgaaactga gcaacgtcaa tcttctgtgt
     1381 ctccagtgcc atacgacatc gttcgcaaac gcacccgggg ccccttcgtt tcataatcag
     1441 gcggctcaat tccaagcttg cacgctgtgc cacacccaaa tccatggctc gaactttgac
     1501 aaggactttt tcaaataggt gctatcgaaa tgcacttgcg atgggaaatc ggcgaacatg
     1561 cagtgggtcg aagccgcgag agagcgtggg gcggccttcc atgaaacttc gggttagaga
     1621 gtctgcactg ggctggacgg ccctgattgt cgcgttgttg ttcgcctttc cggtgcgcgc
     1681 gcagcaggct cagcaagccc cgccgcagac ctcgcagcag acggattcca cctcttccga
     1741 cgaagggatg aaatccggcg attacaatgt tcgacaaagc attgaattcg gttaccgaga
     1801 cagctccatc gacgggaaca taggaaatta cgatacgttt gagaacctcg ggtccggctt
     1861 tcggctattc gattacacgc tggatatgcg ttcggtggac cataaaggct tctttttcga
     1921 cagccttacg ttcagcaact tcggatacgg cggtgatccg aataacgtga cgcgactgcg
     1981 cgtcgaaaag aacaagttgt acgacttccg cgccatgttc cgccgggaca agaatttctg
     2041 gaactacaat ctgctggcga atccgctgaa cccggcaagt tcagttcccg ctctcgccat
     2101 taccagttcg ccgcactccc tggacttggt gcggcggatg ggggattacg atctcacgct
     2161 gcttccggag tctcggctgc gtttccggct cgggtattcc cggaacgtca acgaagggcc
     2221 agggttcacc acgctggaca gcggaaccga acccatgctg acggaaaact tccgatacac
     2281 gacgaacgcg tatcgccttg gagtggacta ccgcttcctg cccaaaacga ccatctcgtt
     2341 tgacgaattg ctgaactact tcaagcaaga cagcaccgtg acggaccaga attttccatt
     2401 tgtgctttcc aacggcacgc ccgccgactt gggaattatt ttcaatacga aaggcaacac
     2461 gccctgcgct actccggtga cgagcgccgc cacaacgcct ccgacggcga acgcgaattg
     2521 caatggattt ctggcctata gccacgtagg tcgtccgcga ggttcgtttc ccaccgagcg
     2581 tcttagcttt cagtccagcc attttaagaa cttagaaatg tccggctcat tcggctacag
     2641 cagtgacagc aattcgactc tcgatttcaa tgaggtcgta aacggctgga ccagtcggac
     2701 cgtcactcga gggagtaccg tagcaggccc cgcgaacgcc aagcgcgtct ccgtaaacgc
     2761 ggattgggcc ggggattatc gtgtgaccga caggctccac attgttgatg aatttcgata
     2821 cgacaattgg cgcattccga gcatgtgggc cacggccgaa acaaatctct ttgcaacacc
     2881 tccggcggcg ccggggcaaa cgggcctatt gcttccaatg gcacaggtca acccggcaaa
     2941 cttcgccacg gtttgccccg ccgctcccta caatggaccg aactgcccac agcatgtctc
     3001 cgggtcactg gccgatgtaa cgaacgaatt tgtttcacga tttctggggc agaacctgaa
     3061 gacgaatacc atcgaggtcc aatacgagat tgcgccgaga attgacgcac acattgggta
     3121 cgtgtatacg gatcggacga tctccgattt caacgccaca tttgatacag gcgaaatcta
     3181 tttcccggga ggagtcaccg gaaatgcggg gaacgatttt ctcgccgcgc gtggggattg
     3241 cgcgttggtc ggaggcgctc tgcctgccgt gtgtacgatg aatccggacg gctcaatctt
     3301 cgagggctcg ccgacgaacc ccgtgccgga agcgggcaat gacacctcac gcggcatcac
     3361 cgacatccac gaaaacgccc tcctcgtggg tatctcggcg cgccccactg acacttttcg
     3421 gatcggcgca gacttcatgt tcggatacaa cgacacttcc tttacacgga taagccccag
     3481 gcaaatccaa agctataaga tccatgcgac ctacacaccg aagcaatggg cgaccatcat
     3541 cggcgcgatc gacattcacg aaaaccgcga caatgtgctc gccgtgaaca atctggagca
     3601 cggccggacc tacagtttcg gcgcgactct ggcgcctagt tcccgtttgt gggttgactt
     3661 cggctacagc tatatggaca tctctattca gactgacatt tgttttgccg agccaggctc
     3721 taccgtattc acaactccgt gccccgtggc gggagcgacg ggtccgctgg gcgcgctttc
     3781 gttctacacg aacacggatc atttcgccta tggccacatg atgtggaagc ccgttaagcg
     3841 cgttaccgct gctctgggat actcgggaac catcgctcag gggaacacgc tcttcctcaa
     3901 cgcgctccaa cccaccggcc cgctcgattt caactatctc aagccatccg cgagcctgac
     3961 gattgacgtc tatcgggggc tgagttacaa gaccgcgtgg aactactatg gatataacga
     4021 tcacggggtc gctaatccaa tcggtctggc acaacttccc ctgcaggatt tcaacggaaa
     4081 caacatcacg ttctcggtac gatatttgtt ctaggtttcg taggagagac aaaacgagcg
     4141 tgtgctacgg ttcgccatat caaggaggcc ggtcagatga atttgtcgct tcggattggt
     4201 aaagcattgt tcttggcagt tgcatttttg tttgcgttca gtgccgtcac gcgcgcggat
     4261 gacgcatcga gcctctataa aaccaagtgc gccgtgtgcc acgcgcccga tggaagcggc
     4321 agttctacaa tgggcaaaaa gttgggcgcg aaagatttgc gatccgatga agtccagaaa
     4381 aaaacggacg cggatcttaa tggcgtcata accaatggca tgggcaagac gatgccagca
     4441 tacaagggaa agctaaccga cgacgagatc aagcagttgg tcggtttcat ccgcgacctg
     4501 gggaaaaaga aatagggttt tcaggcggcc aaaagaaaag cttgaccgag cgcgaggaac
     4561 ttttgtggct caaatgctta ttgcccaaat cattcttagc atcgtactcg cctgcatctt
     4621 tttgattcga ccctctgtaa ccaaggccac ggggtggaaa atcgttgctt ttattgggtt
     4681 atgcgttctt ccggctcttt gcattgtcgg agggatgaac atccacatgc agcgctccga
     4741 gcaaacgcga ttttgcatct cctgtcacgc catggcgccg tatgcccgca gcctttatgt
     4801 cgatgacccc agttatattc ccgcgcagca ctttcagaac cacagggttc cggcggatat
     4861 ggcctgttac gcgtgtcacg ccgattactc catatacggg cctctaaaag acaagatgcg
     4921 gggcctgacg cgcatttata tgcaatacgt gagtacacct ccggcaacca ttcgcattcc
     4981 gggcggatac gataacaacc agtgtctgca ctgccacgcg ggcgctcgcg actttgaggg
     5041 aaatgccatc cacagcgcgg ccatggatgc gctcaaatcg aatcagatgt cgtgcatttc
     5101 aagcgggtgt catgacacgg tgcacaacgt gaccgcgctg gaccatgtaa aattctggag
     5161 gcccggccaa tgacattccg gaatcttgaa ttcaagtggt tgcttcgaat ttcaagtgct
     5221 ctaatcatct tgggactgct cgttgagata gcaagtcttc tgtggtttca tccgctctcg
     5281 tttgtacttt tcaccttcgt gggtgcatcg ttaatcggac tgggaattgt ggtctacctg
     5341 gcatctctgg tcttcgttgc gtcttccggg ggcgaaagac acgagtgaat tgctgggtcc
     5401 gcccgttgtt tccgcctctg tcgcgcgttt cgtcacgtat acgccaaccg aggactgccc
     5461 gaattgagct attctcgagc tgttagcttt gtagggacgg tggggatcga aacgcccgtg
     5521 tacgcagaaa cgaagcgagt cacgtgtttt ctgtagagct aagaaaatgt agatgttcgc
     5581 gggcgagcat gcgctcaacc ttttcgtgcc attctgcggc agagggccaa ttaatgggag
     5641 cgcgtctgtt tcgtaacgaa ttttttctaa ctcccagcca gtaaccattg cgtcgatccg
     5701 catacgccgc gccgattggg gagaacaccg attgcaccgc gggcaatctt ccgtgcattc
     5761 cgtcactgtg aatcctttgt agtttgtcat ggtcttgctc ctgtgcttgt gcgtcacctc
     5821 ggagcagttg cgcactccac aagaagcata gcgtcgaatg gtggggcgat gctgtgcttt
     5881 gcatcaccgt aggggggtga cgcctgagcc gcccggccga tggacgaggc cgaaccgagt
     5941 ggattcactt ataagcaagg gtctgccgga ggtattctgc cacgttgcca atgaagcgat
     6001 gaaccgacac ctccgcgatg cggtggccga ccatcgcgtt catggctttt cccagcgcgc
     6061 cgaacggtgg ttcgtaaagt cccgaaaaat ccaactgcgt ttctgtagcg gtcagcgggt
     6121 agactgaaag ctcggccctc atgagcggga acaagcgtgg catagccgca gcctcccatt
     6181 cgaggagcag ccgggtggtg ggattcgcga cgccaccatg gacttgctcc tcaacgctct
     6241 tgacggaaat cttgatatcg gtcttgaccc caataccgcc aacgtcgacg tgcagctcag
     6301 cggcgatcga ctgcgctcgg gaagcagccg ccttcgtagc tgactggaac acggcgagtg
     6361 cgtctcgctt caacgcatcg cggacgcgct cgtagggatg gtttacgtag tcgtagcagc
     6421 gaatctcacg cgctttactc atctaggtcg ttccgttcag attgaccggc taacctcgac
     6481 atcagtctaa atcgttcgcg attattgatg ctacgactct attcgacggc ctgcaaattc
     6541 aatgtaatcc gaacaccgtt caccaggact atgctttggt tgatcaactc ttgactggtg
     6601 atttcgtctg ctgtgaaaga gcgcagcgct ttcacaggac aaataaactc ggccgcctcc
     6661 gaagattcct tttcaccgcc aagatggtgc agaaccgaga aatcgatggc ctcaagttcg
     6721 tcactcgacg gcaacttctg cattaaggct ttcagccgag gcgccagaaa gaggtcaaag
     6781 ctctgcgccg cacgcttata aatcgaggtg ctgctcgatt ggaattgcgt gggatcgcga
     6841 agggtaaact gaagaacaat tctgttgtgg taaacgacaa gagacggctg cgcataatca
     6901 acgaaatgaa actctgctcc gttggtcttc acaagctcat cgagtcgcga ttggaactga
     6961 gactgcagcc gctcggcgtc ggcttgtgta gagcccggcc gcgatgcagt cacagagggc
     7021 gaggcagcaa tcggctgaaa aactgtcgga ggagccgtga ctgacgcagg tgtcgcccga
     7081 ggcattttga cggccaccgc ggcggctgga gccggagaag cagcaggagg gttcgtaacg
     7141 gatgaactaa tcgctccggc ggccgacgag gaaggaacga ccgtcctcgc aggaattgag
     7201 cgggcaagag cttcgacgtc aaacgggtca agttggtcga gggcgagtcc gaaatctttg
     7261 ccattcacat agattctgga gcgattcaaa atgtcctgct gttctcgctc aggcgcgctg
     7321 acaagtgcaa atgcatcagc tctgtcgagc accgctgcga ggatttcctt tccttcgtaa
     7381 tcatagttct tgctggatgt atgcgcgtca taaacgattt cgaacccaat ggcatcgcag
     7441 tccacatcgc gcgggatctg cttagcaacg agccaaagaa tcggggcgat cacatcccga
     7501 aagatccgcg acgcgcgctg gttttgcgtt aactgatccg cgttgtatgc cgctttgtat
     7561 atccccgaga ttttcagcac cacgcgattc tggaaataaa caaattcaat cccgcgcgaa
     7621 tcgaatgaac tttgtttgct gggttcggat tgaacatagc gcgcgagggc gaacgaaaaa
     7681 gggaattttg tcgcagtgat tgcttgatga agcgactcga gctgaggaag atacttggtc
     7741 tctgccgctt tcagcctggg attcaagatt tctgccggac tcacttgagc aaatgtcgca
     7801 ccgcacgtga gcagcagcgc agcgacgatg agaagtgctg tccgggcgtt gtgaactccg
     7861 caacgaagtt tcatgactct ctcacattca cttctggcgt ggccgggacg gcccttgagg
     7921 cgcgggcgcg gcaatcgcgc gcgcatacgc gacgaccgca cggatttcct ctggcgtcag
     7981 agtctggcca taaggcggca tcatcgccga ctttcccatg gccgtgcctc cttgcgcggt
     8041 gaatttctcg aggaatgtat cgcttagcga attcagagta gcgccgtcgt tccatagcgg
     8101 cggagctggg gtcacattgg aacgattcga gggtccggca ggagtttggt ctgcgtggca
     8161 ccaaacacat tgctggtaga agatctgctt tccctgagcc gcttcgtagc tgagcggaat
     8221 aatccgctgt gcgtcattcc aatctggctc cgtgtcataa gcattctgca acggatcgga
     8281 ttttacgggc acagatttac gggcgcagcc cgccagtaca gccaatccaa gggcggccga
     8341 aaacagaagt gcgccggctc tcattttgtc cccctggtgc tcgccgctgc gccgcccgca
     8401 ggagcacttt gcttcaaagt caacaaataa gccgtcagag cttgaatttc atccgcaatg
     8461 aaagcctgct tcggttcaat cgttccaggt acaagcgcct gcggatcgcg aagccatgcc
     8521 tcaatccatc cgggcgtgag ccagttgccg gcgttgccta aacttggtcc cacgtatccg
     8581 ccggcggagc cgatcgtatg acatgactgg cactcatact ttttttcgta gagctgcttg
     8641 ccggccgccg ccatcgccgg ggtgaattcc ttgcgatcca atgcgtccac ggcagggctc
     8701 tgcatcaccg tttcaaaata gtccgcaaag acggccgcgt cacgttccga catattgaat
     8761 tgcggcatgc gaagcacaag cgtagggcgc agagtctgcg gattctccat aaactggagc
     8821 agccactgcc gtgttgcgcg gctcccttca taagaaagat cgggcgcaag agtgccgccg
     8881 aagccattga atttgtggca cgcgtaacac ttgtagcgct catagagctt cccgaattcg
     8941 cccgcgggat gaaattccgg tttctggcgc gcgacgatca atttctccat tccgcgtgtc
     9001 gccggcggtc cggtcagact caacagagcc gtcgtaacgg cgtccagact tgcttggtcc
     9061 agatgatatt gcggcatgcg cccggcagga ttgaccgaga ttggactcga tatcttcgcc
     9121 tgtatatagg aaatcaatgt gtgtggaatc ttggccgcac cgaaatccag ttgggaaata
     9181 ttgcgagcac ctattgtgga gaggtcaggg ccaaaatcct cctgcgggcg aaggccttcc
     9241 gtcacatggc aactcgcgca gcctttatca acgaataggc gccgcccagc atcgatctcg
     9301 gtttttgtcg gtgaaccaag cttcggcaca tccgcgagca gatccggatc ggtgagcttc
     9361 gtttccatgt aatgcgtcac ttcgtagagg tcggcatccg accattcgta gcggggcatc
     9421 tggctgtggg gcagatagtc ttgcgggttg cgcagccaag cagcgagcca atcgggattg
     9481 accttgctgc cgatcttggt caattccggc ccgatatcgc cgccgataag cgaagtttcc
     9541 ccgacgcgag tgacggccaa cgaatggcaa ctggagcaga acatctgccg gaaacgcgct
     9601 tcgccttggt cggcgacatc cggccgcttc gacaaagagg cgacaattcg aggatcgaaa
     9661 tgataaggct ggatcggatg cggattccgc gtactcaaat atgcggagag gtcccgcagt
     9721 tccggatcgc tcagccgaaa cgtaggcatc tgcggctcgc cgaaatccac accgtccaca
     9781 gttacgctgc catttgcatc cgtgatggtt cgaggttcct tcagccattt gtaaagccac
     9841 tcgcggctca ccttggtgcc aatatccgtc aggtccgggc cgagcatcgc tgggcggtca
     9901 atgccggcga tcttgtgaca tcctgtgcag tcgaagcgag ccagcaactg gcgtccctgg
     9961 tcgagttgcg gagcttgagg aagatcgtcg cggtggcagg ttccgcagga agcttcaatg
    10021 aaatggatgg gaagaattgg ctgttcccat gcgacggtag tctcgtgggc ctccgccact
    10081 tcggttgccg agccttggcc gcggtggcag acgacgcagc cccatgcttc cgtgccatgc
    10141 ggaatcgggg gatgcgcgcg gaatggttcg ggtacggaag cgcccgcaag gctcggttcg
    10201 gcgattccca aatggcacgt cgtgcagcgg tccactactc ccaggtcagg gagccaaatc
    10261 tgataaatgc cgctgaccgt gcggccccgc agcgccgctg cgttcggccg cccttcaatg
    10321 aatttgaaat attgccgctg gtaatggcgc cactgcctga aaaaatcctt cgtcggcgcc
    10381 acagcgagcg aaagcgtgag cagcaggctt acccaggcaa atcgccgggc cggatcgcca
    10441 aaaaaaacgc ggcgaaccca accaacgagt ccttgcgcac ttcgccgatt caccaaggcc
    10501 agatccaggc ccagcctggc ccccggaaaa atgtgccgac ggaagtcaat acaaccgctt
    10561 ccatcagaaa ccaagtcatg atccagaaag agagcggctt cgagctgagc cagcggcgaa
    10621 atcccctatc gcggcgccct tcatttgccg cggcgagtcc catgaaaatg gcgatgagcc
    10681 aaaccggaag cagcgcatcc cacgcatcaa agcgaatcag cagcacggtg agcgccgcca
    10741 gaatgatgcc cagaatcgcc agtcgctgcg atttgtggtg aacccaaagg ttctcgtcct
    10801 tgatattcac gttaaaaaaa ggaaccacga tcaaggccac gacaatcagc gtgggaagaa
    10861 tgatgccggc gacaaatggc ggaaaataat gcagcaactc ctgcaatccg gtgaaatacc
    10921 atggcgcttt ggccaacttg ggagtttgct gaggatcggc gagcaattcc aaaggtgcat
    10981 tccaccagag cgcgatccag accaggacgc tcgctaatag ctctatggcg atcgtttcgc
    11041 gaaacaacat ttcccagtag ggaatggatc tttcttcatc gattcgtcgg ggtaatgtcg
    11101 cttcgcgggg cgcgctggga tcgctcgtca tggtttctct acatcctttt catgctcgat
    11161 gtacattccg ccgtccttgt gcaaacgcca cagatggatc gcgacaagaa gaatgacggc
    11221 cagcggcaga atgacgacgt gcaacacata aaagcgcagc aacgcgtttg cgttcacggt
    11281 gtgtccgccg agaagaatga agcgcagcct ttcaccaata aatggggtgg atgagatgat
    11341 gctcgatccc accgtcgtgc cccagtacga tagttgatcc cagggcaata agtagcccgt
    11401 gtagctggcg agaagcgtca gcagcagaag gcacacaccg acgacccaat tcaattcccg
    11461 cggcggccgg taagcgcctc ggtaaaacac tttggccatg tgtgcgaaca ccaggaacac
    11521 catcgcgtgc gccgaccagc ggtgcagatt tcgcaagaag actccggacg acacgacgaa
    11581 gtgcaggtct ttcatgtcgg cgtagccttg cggaatcgag ggatggtaat acagcatcag
    11641 aagcaggccg gtgatgagct ggatggaaaa agtgccaagc gtcagcgctc cgagatacca
    11701 cgtgtagcga aaaccgagct ggcgctcgcg cacgcgagcg gagaaaatct ccttgaactc
    11761 gtcgcgccaa cgtacaagcg catgtatggg gtttcggatg gctgtgccgg ctggtggagc
    11821 aagtggtttt tgcatcttca aagttggacg tattcgcttt ccgatgcgat atttgtggaa
    11881 cgatccacga tgaggtcgcc atcattgcct atccacatct tcagccactg caacgggcgc
    11941 ggggcggggc cgcccattac cttaccgtcc ggtcgaaatg tactgccgtg gcatggacac
    12001 gcgataatcc cttcatcttg tctccatacc gttagacatc ctagatgggt acaaaccgaa
    12061 ctcatcgcat agaacccctt ctgcgcatgg acgacgaaaa tcgcagtccg tgggtccaag
    12121 gttaccgagt cgatcgggaa atgttcgggt ctgccaacgc ccacgatagg cgatggttca
    12181 tagaggacgt tcggcgacag gtactgcaca gcaaacaccg tcgcaccagc tccggcaaca
    12241 gcaagcgagc cggcccccag cttgacgaaa aactggcggc ggcttagaaa actctcttca
    12301 tcgttgctcg ccgccaggtt tttgttttcc ggctcgctca tgcgcactcg gcctcaacgt
    12361 ttcttacttt ttcaccgtca aatcaacatt caacgcgcca ctcccaacgg taatcgactg
    12421 ggtcgtgggc ttcccctctt cgctccaggt tttcagagtg taggttcccg gcggaacatt
    12481 cttaatttca aatgagccgt ctttgtcggc aacagcgaag taaggcgtgg ccacaacaac
    12541 gacgtagccg gacatctccg ggtgaacgtt acacagcagc gaggcggctc cgagatcatt
    12601 gaattgaaat gattttctct caccctttgg ccaagtcccc aggttgtggg cgagtttctt
    12661 gttgccactg atcgacggcc agtaaacatt gtgcccaact gggtcgctat ttagaaaatc
    12721 cacggtggtc ccctgcaaaa ccactacgac gtggggaacg aacgtcattt tgcgctgatc
    12781 gactacgatg tgttgcgcag gtgcatcgaa atgcttgccc ggtatcgcat cgacgtagac
    12841 ggcgatgtct tgggcagagc gcagcccttg caccgaaacc gttcctttga tgtccccagc
    12901 tcgagcaacg ccgttaaaca gcagaggtac gaagcaaatt gccgcgagta ctaccgctct
    12961 tatgacatgc ttcattcaaa ttcccctttc agctgatcta atttcttttc ttatcggacg
    13021 actaagtctt caaaatacaa agtcagtccc gacaacaaac ccgttgggac ggaagaaagt
    13081 aattgttccg gggactggct cctcgaagcg gcgttgatac tcaaacgcca gcttgatatt
    13141 ggcgcggacc agcacttgaa ccccggggct aaagcggttt cgggtagccg agcgcgaaac
    13201 cccgttcagg aaatcggttg gcgaattcac aacgtcgtag cgcatcaaac cgatcagcca
    13261 cggatacacc cagtattcag cctccgcaaa accgccgctg aacgtcacgg gcgtcgccga
    13321 tacaaagcct gttccaccag aattgagagc ctcgttcgta tcgtgaccgt acatcccgag
    13381 cccccatagc tcgaactggc gatatttgaa acgaaagtcc gcgccaaccc ggtaaaatgg
    13441 ttcgcggatt gtcgacagac ctgggaagag cgcaccgtcg acattcagtt cgttgcggcc
    13501 gtaatagcca aaggctccga gcgtca
//
LOCUS       DATNDT010000494         5430 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4167989, whole genome shotgun sequence.
ACCESSION   DATNDT010000494 DATNDT010000000
VERSION     DATNDT010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5430
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4167989"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..2680
                     /locus_tag="VN788_16225"
     CDS             371..2680
                     /locus_tag="VN788_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HXU22131.1"
                     /translation="MQRRDFLKSSGAVVASGIIGKNALAQTLSASDSGRMMLPINRNW
                     LFRPEAPQGFQARDFNESGFQKVAIPHTNKLLPWHGFDEKSYEFISAYRRHFKLPREA
                     KGKHVFVDFEGAMTASTVWINGQNLGEYKGGYTPFSFELTPHLDWDGDNVLAISLDST
                     ERPDIPPFGYEIDYLTFGGIYREVWLRIVPSTFIENVFAKPKDVLSGKPMLDVQCFIQ
                     QLEASRENLSLEVELRDGDRTLAKTTQRVMPSSPGTEPAATNVHLENLGAVKLWELAQ
                     PNLYTVRVKLMRGGSVIDEDTRRVGFREAEFTDVGFKINGKVVKLRGLDRHQTFPFVG
                     QAMPARVQRRDADILRKNLKCNIVRTSHYPQSRHFLDRCDEIGLLVLEEIPGWQHIGD
                     EAWKTISVDNVRRMIRRDWNHPSIILWGVRINESKDDHDFYTRTNAVAHSLDPTRQTG
                     GIRYFQSSEFLEDVFTMNDFGFPLKPPNHPRYLNTEFVGHTYPTKTIDNVERLREHTL
                     RHARIHNQLASNPQYAGGIGWCAFDYNTHRDFGSGDRICYHGVTDIFREPKPAAGFYK
                     SQCDPAEEIVLEPAFHWARGDESIGFTTAVVCSNCDHVKIYIGQQLVEADPDRTEFGN
                     LPYPPFIVKLNELFHKWGDLRIEGYLKGNKVITRNFSGRGIDQKFALLPDDKSLIADG
                     ADTTRVVLRVTDEFGAIRPFANSAVKLEMDGPATLIGDNPFSLIGGTGAVWIRAKEQA
                     GTVRLVATHPELGSQEVHFEITAAPEETA"
     gene            complement(2696..3895)
                     /locus_tag="VN788_16230"
     CDS             complement(2696..3895)
                     /locus_tag="VN788_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22132.1"
                     /translation="MLPRDLKAEQFAAYPPEARKLMLSDIAALRRLPLSFLPSLLREV
                     IEYDFKFPAERFDLDRELENLKSLDDAQTEEWFGSFAQIHLSPQLEELNWANSPGLFV
                     ERLSAHLWATHQLDAFRAAATGYQERLRKAFPLPLPSLPRVGIAVIGQGVTEYSAPIL
                     RKLRPHGVYFSRVNPDNGLKQILDAVAARAKSHPTAYGHWYIDGGQEAAHDPALTCVS
                     YNALASVRALLLRKIHSQIERPGMGPEELRTLLAKMRPEDLGFDSSGDAVLNRYQMKV
                     LTEGSGTQIFSTTFAQWAAREALRRAQPLTLLVRFAPRQRQKPMNEMLSKVSDKEDMD
                     LVGSLVDADMAAYYNWLNQQRLPGAEQSSFVAWFEGHNQAVAIGPTMPRGTQSSSPID
                     LKELLPA"
     gene            complement(3903..5096)
                     /locus_tag="VN788_16235"
     CDS             complement(3903..5096)
                     /locus_tag="VN788_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22133.1"
                     /translation="MSFRNSPLELLESERGKTPRELAFQLSCNRRDFLRTAAGLTVGG
                     ALLGHSPFMQAALLPKKKKTVVITFGGGARDQETFAPEGQENIPHLMRELIPQSSFFT
                     QVVNRGILGHYVATASLATGVYETFNNFAAVPPEYPTIFEYFRKDLKRPASDAWVVAP
                     SNGFNRIGESSYRSYGQGLGASVILPKHLLAAAMEGRVGDLEPLVRDNYETPYYAPEI
                     RGREFELQQLESILKLSVDDFKAHARTLASPDELSVYIARQLMRQIAPSLLWITMHDI
                     DIAHAGTYSLYVDGIRRTDRLCAEIWKTIQTEPEYAGNTTLFILPDFGRDADEDSGGN
                     GFQHHRTGDALSRTTWMLALGAGVREGVVYDRRVDSTDLVPTVGAMMGFTPALCQGKP
                     VSELL"
     gene            complement(5093..>5430)
                     /locus_tag="VN788_16240"
     CDS             complement(5093..>5430)
                     /locus_tag="VN788_16240"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU22134.1"
                     /translation="KKAIGLDPTYAEAHLQLGNLYSDQTKYAEAIPEYKLALEHNSNL
                     PDAHYRLAQAYVHTSQKDVAQKQFKIYQEQRAEHLAELDKQRAEIRQFVYSEKDSQLH
                     QGGSGKASE"
BASE COUNT         1233 a         1482 c         1469 g         1246 t
ORIGIN      
        1 gctcagctgg atagagcggt tgcctccgga gcaacaggtc gggagttcga atctctccgg
       61 gcgcaccata ccttccattc gcagccgctg attttcgcct tcactcaatt cacagggatc
      121 agatccgtcc gactctgccg gggcgggtca atcaacccat tcggggcgag tgtcgggtcg
      181 ctgttaagca ccacactctg cccgtccgag ctaatccaat tatgcgtgaa ctgattcgag
      241 gtttcgatct tcctggacgg tgttgagagc tacgcacgag ttgcagagct cataaggtta
      301 accgcttcaa ctgttgcatt tacaagggaa tacacttaac atggctatcg caaaaccatg
      361 aggtatcaac atgcagcggc gcgattttct taaaagttca ggggccgtag ttgcgagcgg
      421 aatcattggt aaaaatgccc tggcccagac tctttctgct tcggattcag gacggatgat
      481 gctgccaatc aatcgaaact ggctgtttcg tcccgaggcc ccgcaaggtt ttcaggcccg
      541 cgattttaac gagtcaggat tccagaaggt cgcgattccc cacaccaaca agcttctgcc
      601 ctggcatggt ttcgacgaga aatcctacga gttcatttcc gcttatcggc ggcattttaa
      661 attgccccgg gaagccaaag gcaagcacgt ttttgtagat ttcgaaggcg cgatgaccgc
      721 ttctacggtc tggatcaacg gccagaacct cggcgaatac aagggaggat atacaccatt
      781 ttcgttcgag cttaccccgc atctcgattg ggacggcgac aacgttctgg ccatctccct
      841 cgattccacc gagcggcccg acattcctcc ttttggctac gaaatcgatt acctgacctt
      901 cgggggaatc tatcgcgaag tctggctccg aattgttccc agcacattta tcgagaacgt
      961 atttgccaaa ccgaaggatg tgctctccgg taaaccaatg ctcgatgtgc agtgttttat
     1021 ccagcaactg gaggcgtcgc gagaaaatct ttcgcttgag gtcgaattgc gtgatgggga
     1081 ccgcactctg gcaaagacca ctcaacgagt catgccttca tcgccaggca ctgagcctgc
     1141 cgcgaccaat gtacatctgg agaatctggg tgccgttaag ctttgggaac tcgcccaacc
     1201 gaacctttat acagtgcgtg tgaagttaat gcgcggcggc agtgtcatcg acgaagacac
     1261 gcgaagggtg ggattcaggg aggcggaatt taccgatgtt ggtttcaaga ttaacggaaa
     1321 agtcgtcaag cttcgcggac tggatcgcca tcagacgttt ccgttcgtag gtcaagcgat
     1381 gccggcacgc gtgcagcgcc gcgacgccga tatccttagg aagaacttga aatgtaatat
     1441 cgtgcgcact tcgcactatc cgcagtcccg ccattttctt gatcgctgcg acgaaattgg
     1501 actgctggtg ctggaagaaa ttcccggatg gcagcacatt ggagatgagg cctggaaaac
     1561 gatttcggtt gataatgtgc gacgcatgat tcggcgcgac tggaaccatc catccatcat
     1621 tttgtggggc gtgcgcatta atgagtcgaa agacgatcac gatttctata cccgcaccaa
     1681 tgcggtcgcg cattcgctcg atccgacgcg gcagaccggg ggcattcgct acttccagtc
     1741 atcggaattt ctggaagatg tcttcaccat gaatgatttc ggattcccgc tgaagccgcc
     1801 aaatcatccc cgttacctca acacggaatt cgtgggacac acgtatccca ccaaaacgat
     1861 tgataatgtg gagcggcttc gcgagcatac tctgcgccac gcgcgtatcc acaatcaact
     1921 agcatcgaat ccgcagtacg ccggcggcat cggctggtgc gcgttcgatt acaacacgca
     1981 cagggatttc ggatcgggcg accgcatttg ttaccacggc gtgacggata ttttccgcga
     2041 gcccaagccc gccgccgggt tctacaaatc acaatgcgac cccgcggaag aaattgtatt
     2101 ggagccggcc ttccactggg cgcgcggtga cgaatcaatt ggatttacga ctgccgtggt
     2161 gtgctcaaat tgtgatcacg tcaagattta cattggtcag caactggtag aagctgatcc
     2221 cgatcggacc gagtttggga accttcccta tccgccattc attgttaagc ttaacgagtt
     2281 gttccacaag tggggagatt tgcgaatcga aggctacctc aaaggcaata aagtgattac
     2341 tcgaaatttt tctggccggg gaattgacca gaagttcgcc cttttgccgg acgacaagag
     2401 cctaatagcc gatggggccg atacgactcg agtcgtactg cgcgttacgg atgaatttgg
     2461 cgccattcgt ccctttgcca acagtgctgt caaattggag atggacgggc cagctacgct
     2521 tatcggcgat aatccatttt cgttgatcgg tggcacgggc gcggtctgga ttcgagcgaa
     2581 agaacaggcg ggaacggttc gcctggtggc gacgcatcct gaactgggca gccaggaagt
     2641 acactttgaa atcacggccg ctcctgagga gacagcgtag ctttcgaatt tcggttcaag
     2701 ctggaagcaa ttctttcagg tcaatagggg aagaggactg cgtgccccga ggcatagtgg
     2761 gaccaatggc gaccgcctga ttatggcctt cgaaccaggc tacaaatgag gattgttccg
     2821 ccccgggcag gcgctgctgg ttgagccagt tgtaataggc cgccatgtca gcatcaacca
     2881 gcgaaccgac aagatccata tcttccttgt cggacacttt cgataacatc tcattcatcg
     2941 gtttctgccg ctgacgaggg gcgaaccgga ccagcagcgt caacggttga gcgcgccgca
     3001 aagcttcgcg cgcggcccat tgcgcaaacg tagtgctgaa tatctgcgtt cccgatccct
     3061 cggtcagtac tttcatttga tacctgttca ggaccgcgtc tccgctactg tcaaaaccga
     3121 gatcttccgg ccgcatcttc gccagcagtg tccgcaactc ctcgggaccc attcccggac
     3181 gttcgatttg actatgaatc ttgcgcaaaa gaagtgcgcg caccgaagca agcgcgttgt
     3241 aagaaacaca agtaagcgcg ggatcgtgag cagcttcctg tccaccatcg atgtaccagt
     3301 gaccgtacgc tgtgggatgc gacttcgctc ttgcggcaac tgcatcgagg atttgcttga
     3361 gcccgttatc cgggttaacc cggctgaaat atactccatg ggggcgcagc ttgcgcaaga
     3421 tgggcgcgga atattcggtt actccctgac cgatgaccgc aatcccgacc cttggcagcg
     3481 aaggcaatgg cagcggaaag gctttacgca ggcgctcttg atatccggta gccgcagcgc
     3541 gaaatgcgtc caactgatgt gtcgcccaga gatgcgcgga aagccgttcc acgaataggc
     3601 caggagaatt tgcccaattg agttcttcca gttgcggcga tagatgaatt tgcgcgaacg
     3661 acccgaacca ctcttccgtt tgcgcgtcat cgagggactt caaattctcc agctctctgt
     3721 ccagatcaaa tcgttcagca ggaaatttaa agtcatactc gatgacttct cgcaacaggc
     3781 taggcaaaaa actcaacggc aggcgtcgca gcgcggcaat atccgaaagc atcaacttgc
     3841 gcgcctctgg cggatacgcc gcgaactgct ctgcttttag atcgcgagga agcatatggt
     3901 gatcacaaaa gctcagacac gggcttgccc tgacacaacg cgggcgtgaa acccatcatt
     3961 gctccgaccg tgggcacgag gtcggtcgaa tctactctgc ggtcgtaaac cacgccttcg
     4021 cgcactcccg caccgagagc caacatccag gtggtgcggg aaagagcatc tcccgtgcga
     4081 tggtgctgaa aaccgttgcc gccggaatct tcatccgcgt cacggccaaa atccggcagg
     4141 atgaaaagcg tcgtgttgcc cgcatattcc ggctcggttt ggattgtctt ccaaatttcc
     4201 gcgcaaagcc ggtcggtgcg ccgaatgccg tccacataaa gcgaataagt tccggcatgg
     4261 gcgatgtcaa tgtcgtgcat cgtgatccac agcagactgg gcgcaatttg ccgcattagc
     4321 tggcgagcga tgtacacgga gagttcgtca ggactcgcca gcgttcgcgc atgggcttta
     4381 aagtcatcca cggaaagttt gagaatgctc tctaactgct gcaactcgaa ttcgcggcca
     4441 cgaatctccg gcgcgtagta gggcgtctcg taattgtcgc gcacaagcgg ttcaaggtcg
     4501 cccactcgtc cctccatagc ggcggccaag aggtgtttcg gaaggattac gctggcgcct
     4561 aatccctgtc cgtaggagcg atagctgctt tcgccgatgc ggttgaaacc attgctgggc
     4621 gccactaccc aggcatcgga tgccggacgc tttaaatcct tccggaaata ttcgaagatg
     4681 gtcggatatt caggagggac cgcggcgaag ttattgaacg tttcataaac tcccgttgca
     4741 aggctggcgg tcgcaacgta atgaccaagg attcctcgat tcacaacttg ggtaaagaaa
     4801 ctggattgcg gaataagctc tcgcatcaga tgagggatat tttcttgccc ctcgggcgca
     4861 aaggtttcct gatctcgcgc tcctccgcca aatgttatga ccacggtttt ctttttcttg
     4921 ggaagtaatg ccgcctgcat gaacgggctg tggccaagca gagcgccccc aactgtcagt
     4981 ccggcggcgg tccgcaaaaa atcgcgtcgg ttgcaagaga gttggaacgc aagttcacga
     5041 ggcgtctttc cgcgttcact ctccagtagc tccagtggcg aattgcggaa gctcattcgg
     5101 acgccttccc actgccgccc tgatgaagct ggctgtcttt ttccgaatag acaaactgcc
     5161 ggatttccgc ccgctgcttg tcaagttccg cgagatgctc tgccctctgc tcttgataaa
     5221 tcttaaattg tttctgcgct acatcttttt ggctggtatg tacataagcc tgcgccagcc
     5281 gataatgagc atccggaagg ttggaattat gttcgagcgc aagtttgtat tccgggatgg
     5341 cctcggcgta cttcgtctgg tctgaataga ggttccctaa ctgcaaatgc gcctcagcgt
     5401 aagtgggatc gagcccaatc gctttcttta
//
LOCUS       DATNDT010000495        16841 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2686495, whole genome shotgun sequence.
ACCESSION   DATNDT010000495 DATNDT010000000
VERSION     DATNDT010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16841
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2686495"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="VN788_16245"
     CDS             <1..534
                     /locus_tag="VN788_16245"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXU22135.1"
                     /translation="ASTGVYTIMEQLNVVYDVKDQRPLWEARTTAILLMVFFVLLAIG
                     SLSLVIFGGVVQSWVASMIGWSRPLLIFFATLRWIIIAAALLLSLAVTYRYGPDVNLE
                     FRFISAGNVAAAILIALASIGLRLYVSMFGSYSATYGNLAAMIILMLWMYMAGLALLA
                     GCEINAISHRNKPEQPK"
     gene            complement(595..3882)
                     /locus_tag="VN788_16250"
     CDS             complement(595..3882)
                     /locus_tag="VN788_16250"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU22136.1"
                     /translation="MKSIPALLAVFLFAGALFAQSISTSQIAGVVRDASGLPIPGVEV
                     TVTQTQTGVSRTTQSGPDGAYLLLNLPIGPYRMEVKKEGFSTYVRSGIVLQVNSNPAI
                     DVSLQVGTVSQQVVVEAAASMVETHSTGVGQVVDPQRMVDLPLNGRNVTDLIYLAGAS
                     ATAPNADLVSTKNYPNEAPISIAGGLATGTTYLLDGGEYNDSVNNLSLPLPFPDALEE
                     FKVETSALPAQYGEHSGGAVNAISKSGSNNFHGGAFEFVRNYIFNAKNFFAATRDSLK
                     RNQFGGELGGPILKDKLFFFVGYQGTLIRSNPASSFSFIPTAQMLAGDFTTITSPACN
                     GGKQITLAPPFVNNMISPTAFNSASLKMMTFYPSTSDPCGKISFGLLQNQDEHQGLAR
                     VDYQLSSKQSVYVRYFGTHALVASPFDGKNPLTETLSGADDLVNSIVFGHTYVFGPSL
                     VNAFHATFNRSAVTKTQTPSFDANTLGIAMNTLVPGHIVASVSNAIYSSALFSYAATD
                     PTQSGQLSDDINLIRGAHQLQFGADFIHYSENVFGPLFGDGFFVFTGQFTGLPMADFL
                     LGNVASFTQGNPQFDYSRYNYFGIYGQDSWKVNSHLTVNAGLRWEPYFGGSLKTGEVS
                     HFDPALFTAGVHSTVYVNGPAGLLFPGDPGFTTGKRPSHTSWKDFAPRFGLAWDPQGN
                     GKMTVRASWGIFYDMPQTLFGYGFSEEPPWGEGISRTNVAFDDPWGGYPGGDPFPITL
                     SPNFAFPTFGTYANYPLHLKITYLEQWTLGVQRQFGANWLASASYLGNNTIHLWGDNP
                     VNAPVFMGLGPCTINGISYPTCSTTANENQRRPLYLQNPAQGQFYGAILQLDPSSTAS
                     YNALLLSLQHRLSNHFTVLANYTWSHCITDPFTSELDSTATQYTDPSNRRYDRGNCVG
                     IDHRQNFNLSGVAESPRFSEGWLQKVAGNWRLSMALQAKTGSYFNVTTGTATTLTGTG
                     APRPNQVLANPYCADPKPSCWLNRAAFATPAPGTFGNTPAASIHGPGYLTINMAISRL
                     IFIHERQSLELRAEAFNLLNNLSPGNPLPGSSLSTVSTALNSSNFGRLQIAADPRIMQ
                     FALKYAF"
     gene            complement(4154..6415)
                     /locus_tag="VN788_16255"
     CDS             complement(4154..6415)
                     /locus_tag="VN788_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015029909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HXU22137.1"
                     /translation="MRRFAVLSIMPILFVLLCGRADEEPLQGFAPQSSSAERQWEGKF
                     RALPSPDNQREYMRRLSAHPHHVGSPYDRDNAEWVLSKFKEWGWNADIETFDVLFPTP
                     KERLLEMTQPTHFAAKLQEPAVPGDPTSNQQSEQLPPYNAYSTDGDVTAPLVFVNYGL
                     PKDYEQLDRLGVSVKGAIVIAKYGNSWRGIKPKVAAEHGAIGCVLYSDPADDGYSVED
                     VFPKGPMRPSDGVQRGSVMDFPSSSPGDPLTPGVGATKDVRRLAIKDAPSITKIPVLP
                     IPYGDAQPLLAALTGPVVPKEWRGSLPITYHVGPGAAKVHLKVSFHWDIKTVYDVIAR
                     LPGAEQPDQWVIRGNHHDAWVNGAEDPTSGQVALLEEARSLGELVKQGWRPKRTIIYC
                     AWDGEEPMLLGSTEWAEAHDKELQQHAVAYINSDGNERGYLNMVGSHTLEHFVNGVAQ
                     DIQDPETKLSVGRRLRLHEIEEAKNAEDRKEVRERADVRMGALGSGSDYTAYIDHLGI
                     ASLNLSYGGEDESGIYHSVYDDFYWYTHFGDTDFVYGRALSQTIGTAMMRLADADLLP
                     FEFTDFADTIHLYVKQLKKLADDARDEALERNKEIDEGLFAAVNDPRHPKVVPPRENV
                     PPHLNFAPLDNSADALALSAQRYHAAIGKVWSLGLSAASLHDLNQKLMESERRLTTQQ
                     GLLRRPWYKHMIYAPGVYSGYSAKTIPGVREAIEQKHWDEANSEIVRVAAVLDAETAL
                     INSAVQDLQQPKP"
     gene            complement(6575..7861)
                     /locus_tag="VN788_16260"
     CDS             complement(6575..7861)
                     /locus_tag="VN788_16260"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HXU22138.1"
                     /translation="MDLTLFFAILGGLLVLAFVANRLVRITRVPDIVILMATGVLIGP
                     VFHWVNPDVFRGATHGLGTLALILILFEGGLELKLREILRHFGSGFFLSLFSYLLCMG
                     AVATVCRFAAHLSWLNALLIGAVLGCLSSSIVFPALQQIHLRREVKVTLLVEASLGDA
                     FAVLAVTTLLNIASGGSVAPRTVAWSLASSLVLAVAGGILAGMLWSYLLPFLSDERFW
                     HVLTFAAVLLVYAGVHFLKGNDLISVLVFGLTLGNLPTVRKRLHLDETAATDWFDEMP
                     VKSGGRPIDDFHHEQLLTFHGELAFLLRTFFFVLLGMLVDYPGIRRNPWLVLLCFIAI
                     MVARVVAVGVGRLGWRTFSALELELIVWFAPRGLITAVLGIDVLEARGDTFAFLPSLA
                     FGIILLTSVTLLIGSVRARNLPASDPEPAEEATTPA"
     gene            complement(8395..9642)
                     /locus_tag="VN788_16265"
     CDS             complement(8395..9642)
                     /locus_tag="VN788_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22139.1"
                     /translation="MPLKPIVFTPHPVWGKTPAELAAMVAGPDPVTGKPMMTEIIGNL
                     TTPLSEGEKKTGIENVSAGPAVYGPDTLDSLQEIFMNNGMTDYMPVIIPTEEKVDAML
                     KGTSHHPDEVLKKAIQGVGYESAQWGFTVRHVAVNAVMAGCRPEYFPVVLALASAGQL
                     GFFGSTTSGGSAIVINGPIRDKLNMNYGIGALSPFAQANATIGRSWTLMGKNLTSGGI
                     PGDTYQGSQGNQLDYNNCIIAEDEKSSPWTPFHVQQGFKPEENVVSLFLGWDIRQGHG
                     ATGAGAITDPMFDEQISSMFHTMAGTFGALVICDPLVAKRLAAQGYGTKEKLSEWLWK
                     NTMRTAKEYRESSLSYVYTYPLALEGREPYATWWKAPEDTLIPWFPKATSINFVVCGG
                     QTNAFFQVGNMSHVKSASIDKWM"
     gene            complement(9766..10173)
                     /locus_tag="VN788_16270"
     CDS             complement(9766..10173)
                     /locus_tag="VN788_16270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22140.1"
                     /translation="MRINRRKLLSLLGTSPVLLAGSAAEAQAVKKVVHSGVGSEAKFT
                     AVSPKGIPPAVQLYSMAPRLDTLDGKTVYLVDTGFFGGGLLLKEMQGWFQQNMPSVKT
                     VFRKKAGGWAADDPPLWAEIKAKGNAAIMAIGH"
     gene            complement(10521..13883)
                     /locus_tag="VN788_16275"
     CDS             complement(10521..13883)
                     /locus_tag="VN788_16275"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HXU22141.1"
                     /translation="MKRILAQLVIVSALCLGYCAGVFAQATSQISGTVKDASGGVIAG
                     AQITVTQTATGVTRTATSDANGVYSLPSLPLGPYQMEVKKDGFTTYLQSGIVLQVAQG
                     PTIDPVLNVGAVSQTVQVEAAAAMIETSSTGVGQVVNSQSVVDLPLNGRQVTQLITLA
                     GASNAVQYGFGQSPSVGNLVSSKNYPNEALVSVGGGLLNGTTYLLDGGTYNDPFNNLN
                     LPTPFPDAIQEFNVQTSALPAEYGQHSGGAINMASKSGTNELHGDAFEFVRNADFNAR
                     DFFTFSPSDPNGASDNLKRNQFGGTLGGPVKKNKLFFFLGYQGTLIRSAPAAIPAVLP
                     TASQLTGDFRAYEANCFATPQTLKAPFVNNVLSTPVSPQALAFASHFDVGPEPCGNVT
                     YRIIQNQNEHMGVAKVDYQINSKQSLFVRFFGTHSLQPSSFTGSELSVQNAGTDDTVT
                     SAVVGHTYLVSPNALNTFHFAFNTDGVTKFQVPIVTPTDIGVQGMFIALPHYSNINIT
                     GDFQSAGGFATPGLVNTKTYQLKDDFSLTKGSHQLQFGVSFIRPWQTSTFCVFCNGLF
                     TFSGSNTGNAMSDFISGELSSFTQLNLTHDNEYWRSLGIYAQDSWKINSRLTLNYGLR
                     WEPYLAGQLRPNQVTHFVMADFLNNVHSSIFPNAPAGTLYPGDAGFRTGGRPNFTAWN
                     NWAPRIGLAWDPTGSGKTLIRASWGIFYDMPHTLFYFNYSSEPLWGESITITSPSGGF
                     ANPWLGFPGGSPFPTTQNAASAYPLGGYYETVPLHVRNTYVEQWNLTLQKQMGRSWLL
                     KAAYLGNNSIHLWLDQELNPAVFVPGNCVAGQFGLAAPGPCSTTRNTQARRLLTQLNP
                     SQGPFYGASESLDDHGTASYNALIVSAEHHLSSHFSVLVNYTYSHCIADPQTTELSGP
                     IGTKPGDRRFDRGNCPFIDIHHNFNLSGVLQSPRFSSRALQLIAGDWQLAPIVGIHTG
                     SYFTVTTGIDDALTGIPAQRPNQVLSDPYCTPRHVSVVLANNCWLNPSAFAQPALGTL
                     GNSRANSLEGPGYVDVDLSLSRRFPITERQGIEIRWETFNVANHTNFLNPGTSGIAAG
                     GTNGTVLNSSVFGKVQSDVAPRIMQFAIKYIF"
     gene            complement(14145..14555)
                     /locus_tag="VN788_16280"
     CDS             complement(14145..14555)
                     /locus_tag="VN788_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HXU22142.1"
                     /translation="MGNIGGIYVLAFVIGRILVGGFFIIAGVNHFARLKMMAGYAKMK
                     GTPAPELAVGTSGILLLLGGLSLLLGFHPTIGVILLAIFLVPTTLMIHNFWAVQDPQA
                     KMNDLVNFEKNVAILGFLLMTLLITRPWPISLGH"
     gene            complement(14790..14864)
                     /locus_tag="VN788_16285"
     tRNA            complement(14790..14864)
                     /locus_tag="VN788_16285"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14830..14832),aa:Phe,seq:gaa)
     gene            15017..15427
                     /locus_tag="VN788_16290"
     CDS             15017..15427
                     /locus_tag="VN788_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HXU22143.1"
                     /translation="MTEKKIRVLIAKPGLDGHDRGAKVIARALRDAGMEVIYTGLRQT
                     PEMIASAAVQEDVDVIGLSILSGAHNTICPRLMDLLRAKGMNDVIVLIGGIIPDTDIP
                     ALKQAGIAEVFLPGTTTQSIIDFIRERAAARQGI"
     gene            15537..>16841
                     /locus_tag="VN788_16295"
     CDS             15537..>16841
                     /locus_tag="VN788_16295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HXU22144.1"
                     /translation="MIDRVAEIKNEEEKIRKGGGAKSIEAQHKKGRLTARERIARLVD
                     PGTLFFELGIYAAFEMYAEWGGAPAAGTITGLGNVAGRRVMIIANDATVKAGAFFPMT
                     AKKVIRAQNIAIENRVPTIYLVDSGGVFLPLQEDVFPDTDDFGRVFRNNAVMSAMGIP
                     QIAAIMGLCVAGGAYLPVMCDHVLMTEGSGLFLAGPALVQTAIGQKVSLEELGGARVH
                     AQISGTADFREPNDEACIERVRALVDKMGHSPGAPFDHIRPEPPAYPPEEIYGIFSAD
                     PSKQYDTREIVARIVDGSRFEEYRAERGQTVLCGYARVGGWAVGIVANQKKNATISAP
                     GTDEKRIEFGGVIYTESADKAARFILDCNQNLVPLIFLHDVNGFMVGKEAEWTGIIRA
                     GAKMVNAVANSVVPKITVICGGTFGAGHYAMCGKAYDPRFVFA"
BASE COUNT         3756 a         4596 c         4957 g         3532 t
ORIGIN      
        1 gcatccacgg gcgtttatac gatcatggag caactgaatg tggtctacga cgtgaaggac
       61 cagagaccct tatgggaagc gcggacgacc gcaattctgc tgatggtgtt ctttgttctg
      121 ctggcgatcg gatcactctc gctggtgatc ttcggaggag tggttcagtc gtgggtggcg
      181 tcgatgatcg gatggagccg gccgctgctc attttcttcg cgactctgcg ctggattatt
      241 attgccgccg cgttgttact gagtcttgcc gtgacttacc gatacggccc cgatgtaaac
      301 cttgagttcc ggtttatttc agccggaaac gtggcggccg ccattctgat cgcgctcgcc
      361 tcgattggac ttcgattgta cgtctccatg ttcggcagct acagcgcgac gtatggcaac
      421 cttgccgcca tgatcatcct gatgttgtgg atgtatatgg ccggcctcgc ccttttggcg
      481 ggttgcgaaa ttaacgcgat ctcgcaccgg aacaagcctg agcaacccaa atagacttcg
      541 cccaggacaa ggaccctccc gcagcgcgga cgccttgctg cgtgccgcct ccgcctagaa
      601 ggcgtatttc agcgcgaact gcatgattcg cgggtcggct gcgatttgaa gtcggccgaa
      661 attcgacgag ttgagagcgg ttgagacggt cgagagcgag ctgccgggca gcggattccc
      721 cggcgaaaga ttgttcagga gattgaatgc ttccgctcgc agctcgagcg actgcctttc
      781 gtgaatgaaa atgagcctgg atatggccat attgatggtc aggtagccgg ggccgtggat
      841 actggcggcg ggcgtgttcc cgaaagtccc aggcgccggt gtcgcgaagg ccgcgcgatt
      901 cagccagcaa gacggttttg ggtccgcgca atagggattg gctaaaacct ggttgggccg
      961 aggcgctccg gttcccgtaa gtgtggtagc ggtgccggtg gttacgttga aatagctgcc
     1021 ggtcttagcc tgcagggcca tcgagagtcg ccaattgccg gccaccttct gcagccaccc
     1081 ctccgagaac ctggggcttt cggcgacgcc ggacagattg aagttttggc gatggtcgat
     1141 cccgacgcaa ttgccgcggt cgtagcggcg gttgctcgga tcggtgtatt gcgtggcagt
     1201 ggagtccagc tcggacgtga acggatcggt gatgcagtgg gaccaggtgt aattggcgag
     1261 caccgtgaag tgattcgaga ggcggtgctg cagggacagc agcagcgcgt tgtagctggc
     1321 cgttgaggac ggatcgagtt gcaaaatagc gccatagaat tggccttgcg ccggattctg
     1381 caagtacagc gggcggcgct gattttcgtt ggcggtggtg gagcaggttg gataggagat
     1441 tccgttgatg gtgcaagggc cgagacccat aaagaccgga gcgttgaccg gattgtctcc
     1501 ccacaagtga atcgtgttgt tgcccaaata actcgccgac gcaagccagt tagccccgaa
     1561 ctgccgctga acacccagag tccactgctc caggtaggtg attttcaggt gcaaggggta
     1621 gttagcatat gtgccgaagg tggggaacgc gaaattggga ctcaaggtaa tcgggaatgg
     1681 atcaccgcca gggtatccgc cccaaggatc gtcgaacgca acgttggtgc gcgagattcc
     1741 ctcgccccaa ggcggttcct ccgagaagcc gtagccgaac agtgtctgcg gcatgtcgta
     1801 gaagatgccc caggaggcgc gcaccgtcat tttgccgttc ccctgcggat cccaggcaag
     1861 gccgaatcgc ggcgcaaaat ccttccaact tgtgtgggaa ggccgcttgc cggtggtaaa
     1921 tccgggatca cctgggaaga gaaggcccgc gggaccattt acgtacacgg tggagtgtac
     1981 gccggcggtg aatagagccg gatcaaaatg cgatacctcg ccggtcttta gggatccacc
     2041 aaagtacggc tcccagcgca atccggcgtt cacggtcaga tgggaattaa ctttccacga
     2101 gtcctgcccg tatatgccga aatagttgta gcggctgtag tcgaactggg gatttccttg
     2161 cgtaaagctg gctacattcc cgagcaggaa atcggccatt ggcagtcccg tgaactgccc
     2221 ggtgaaaacg aagaagccgt cgccaaagag cggcccgaag acgttctcgc tgtagtgaat
     2281 gaagtccgcg ccgaattgga gctgatgggc tcctcgaatg aggttgatgt cgtcagacaa
     2341 ctggccgctc tgggtagggt cggtcgccgc gtaagaaaat agcgcggaac tgtaaattgc
     2401 gttcgacacg ctggccacga tgtgtccggg gaccagggta ttcatggcga tgccgagggt
     2461 gttggcgtca aacgagggcg tctgagtctt ggtcaccgcc gaccggttga aagtggcgtg
     2521 aaaagcattc acgaggctgg gcccaaatac gtaggtgtgg ccgaacacga tcgaatttac
     2581 gaggtcgtcg gcgcccgaca aggtttcagt gagcggattc ttgccgtcga acgggctggc
     2641 caccagcgca tgggtgccga agtaacggac gtagacggac tgcttgctgc tcaactggta
     2701 gtccactctg gccaagccct ggtgctcatc ctgattttgc aggaggccaa aggatatctt
     2761 tccgcaggga tcgctggtgg aaggatagaa cgtcatcatc ttcagcgatg cgctattgaa
     2821 cgctgtgggg gagatcatgt tattgacaaa cggaggcgcc agggtgatct gcttgccccc
     2881 gttacaggcg ggagaggtga tggtggtaaa gtcgcccgca agcatctgcg ccgtgggaat
     2941 aaaactgaag ctcgaagcgg gattggaacg aatcaacgtg ccctggtatc ccacgaagaa
     3001 gaaaagcttg tctttcagga tggggccgcc caactcgccg ccaaactggt tgcgcttgag
     3061 ggaatcgcgg gtcgccgcga aaaagttttt cgcgttgaag atataatttc ggacgaactc
     3121 gaaggcgcca ccgtgaaagt tgttgctgcc cgattttgag atcgcgttca ccgcgccgcc
     3181 ggagtgctct ccgtattgcg cgggcaacgc gctggtttcc actttgaact cttcgagagc
     3241 gtctggaaac ggaagcggca gactgagatt gttcacggaa tcgttgtatt cgccgccgtc
     3301 cagcagatag gttgtccccg tggccaatcc gcccgcgatg ctgatgggcg cctcgttggg
     3361 ataattcttc gtggagacca agtccgcatt cggtgccgta gcggaggctc cggccaagta
     3421 gatcaagtcg gtgacatttc gtccgttcaa gggaaggtcg accatccgct gcgggtccac
     3481 cacctgtccg actcccgtgc tgtgagtttc aaccatcgag gctgcggctt ccacgacgac
     3541 ctgctggctg accgtcccga cttgcagtga gacgtcgatg gccggattgc tatttacctg
     3601 cagtacaatg ccggaacgca catatgtgct gaagccttct ttcttcacct ccatccggta
     3661 cggaccaatc ggcaggttga gaagcaagta tgcgccgtcc gggccgcttt gcgtcgtgcg
     3721 ggaaacgcca gtctgagttt gtgtgacggt tacttcgacg cctggaatcg ggaggccgct
     3781 agcatcgcgg acgactcctg cgatctgcga ggttgaaata gactgagcaa aaagtgcgcc
     3841 tgcaaataga aaaaccgcca acagcgctgg aattgatttc ataccggtcc cctcccagga
     3901 ataaagcaat aagactctga accgaaatcg cttttgttcg atgaacgatc cagatgttgc
     3961 aattccgacg tcgattcgtg gcccattcgt ctgaattgcg cgcccaccat aactcaaaaa
     4021 atgtcccgaa gcatatttat ttgtttaatt tttccttgaa cgcgccgaaa gccgcaaggt
     4081 cacttcaaga tcgaacgcgt gcgaaatctt gaagactaat gctccgcgcg ggtgcggtac
     4141 ccggaggcga cgttcatggc ttcggttgtt gaagatcctg aaccgcggag ttgatcaatg
     4201 ctgtttcagc gtcgagtacc gcggcaactc gcacgatctc ggaattcgct tcgtcccagt
     4261 gtttctgctc aatggcttcc cggacgccgg gaatcgtctt cgcgctgtag cctgaataaa
     4321 caccgggagc gtaaatcatg tgcttgtacc acgggcgccg cagcagtcct tgctgagtgg
     4381 tcaagcggcg ctcactctcc atcagctttt gattgagatc atgaagcgaa gctgcggaaa
     4441 gacccaaact ccaaactttg ccgatggcgg cgtgataacg ctgcgcgctc agagcgagag
     4501 cgtccgctga attgtccaac ggagcaaagt tgagatgggg cggtacattt tctcttgggg
     4561 ggacaacttt gggatggcga ggatcattga cggcggcaaa aagcccctcg tcgatttcct
     4621 tattgcgctc aagggcttca tcccgcgcat catcggctag tttcttcaat tgcttcacgt
     4681 ataaatgaat tgtgtccgca aagtccgtga actcgaaggg gagaagatcc gcgtcagcca
     4741 aacgcatcat ggctgtgccg atcgtttgcg ataacgcacg cccataaacg aaatcagtat
     4801 cgccgaaatg cgtgtaccag tagaaatcgt cgtaaacgga atgataaatg ccgctttcat
     4861 cttcaccacc gtaggacaaa ttcagcgaag cgatgccaag atgatcgata tacgcggtgt
     4921 aatcagatcc ggagcccaac gcgcccatcc gcacatcagc acgctcgcgc acttctttcc
     4981 gatcttccgc atttttcgct tcctcgattt catgaaggcg aagacgccga cctacgctga
     5041 gttttgtctc gggatcctga atatcttgcg ctaccccgtt cacaaaatgt tcgagggtgt
     5101 gagagccgac catattgagg tatccacgtt cgttgccgtc gctattgata taagcgacgg
     5161 cgtgttgctg cagctctttg tcatgcgctt cagcccattc cgtggagcca agtagcatgg
     5221 gctcttcgcc gtcccacgcg caatagatga tcgtacgttt gggccgccag ccctgtttga
     5281 ctagttcccc cagcgaccga gcctcttcaa gcaaagcgac ttgtcccgat gtcgggtctt
     5341 cggcgccgtt tacccacgca tcatggtgat tcccgcgaat gacccactga tctggctgtt
     5401 ccgcgccggg aagcctagca ataacgtcat aaaccgtctt gatatcccaa tgaaagctca
     5461 ccttcagatg gactttggcg gcgccggggc ccacgtgata cgtaatcggc agcgagccgc
     5521 gccactcctt cggaaccacc ggacctgtga gtgccgccag cagtggctgc gcatcgccat
     5581 aaggaatggg gagcactgga atcttcgtaa tgctgggcgc gtctttaatc gccagccgcc
     5641 ttacatcttt cgttgcgccc acaccgggag tgaggggatc gccaggactg gaagagggga
     5701 aatccatcac acttccgcgt tgaacgccgt cagagggccg catggggccc ttggggaaca
     5761 catcctcaac ggaatagccg tcgtcagccg gatcggagta gagaacgcaa ccgatggcgc
     5821 cgtgctccgc cgctactttc ggcttgatgc cgcgccagga gtttccgtac ttagctatga
     5881 cgatcgcacc cttcaccgaa actcccaatc gatcgagttg ttcgtagtct ttgggcagcc
     5941 cgtagttgac gaaaacaagc ggggcggtaa cgtctccgtc agtcgaataa gcgttatagg
     6001 gcggcagctg ttcgctttgt tgattcgacg tggggtcgcc cgggacagcg ggttcttgca
     6061 gcttcgccgc gaagtgcgtg ggttgggtca tctcgagcaa tcgctctttg ggagttggaa
     6121 ataggacatc gaaagtttcg atgtcagcgt tccagcccca ctccttgaac ttcgaaagaa
     6181 cccattcggc attgtcccga tcgtagggcg aaccgacatg gtgcgggtgg gcactgaggc
     6241 ggcgcatgta ctcgcgttgg ttatccgggc tggggagtgc gcgaaatttt ccctcccatt
     6301 gacgttcggc gctcgaactt tgcggcgcaa acccttgtaa gggctcttca tccgcacgac
     6361 cgcaaagcaa gacgaaaaga atcggcataa tgcttaatac agcgaaacgt cgcatcgaaa
     6421 tttctcctat cagtcaatcc cagctagacg cgtttggctg ttcgtgagct gccgcgagct
     6481 acgttctgtc ggaagggtga tgtcgtgctg ccgcgcctcg attcggtcgt gcccatccta
     6541 ctcccatgga gcttaaatgc gcaggctgca acgtctaagc cggggtggtg gcttcttccg
     6601 caggttcggg atcagaggca gggaggttac gcgcgcgcac gctgccgatc agaagcgtga
     6661 cgctggtaag caaaataatg ccgaaggcga gcgatggcag aaacgcaaag gtatcaccgc
     6721 gcgcttcgag cacatcgatg cccagcactg cagtaataag accccgggga gcaaaccaca
     6781 cgatcagttc gagctccagc gcggaaaacg tgcgccagcc caggcgtcca accccaactg
     6841 caacaacgcg cgccaccatg atggcgataa aacacaacaa cacgagccac gggttcctgc
     6901 gaattccggg gtaatcaacc agcatgccca gaagcacgaa gaaaaaagtc ctcaggagga
     6961 aggccaattc tccatggaag gtcagcaatt gctcgtggtg aaaatcgtcg atggggcgcc
     7021 cgccactttt caccggcatt tcgtcaaacc aatcggtcgc ggcggtctca tccaaatgaa
     7081 ggcgcttgcg cacggttgga agattgccga gggtgaggcc gaagaccaaa accgaaatga
     7141 ggtcgttgcc cttgagaaaa tggacgcccg catagacgag aagcacggcg gcgaaagtga
     7201 gaacgtgcca aaagcgttca tcggaaagaa acggtagcag ataagaccac agcatccccg
     7261 ccagaatgcc gccggcgacc gcgagtacca gactggaggc cagagaccaa gccacggttc
     7321 tcggggcgac gcttccccca gaagcaatat taagcagcgt ggtgaccgcc agcacggcaa
     7381 aggcgtcgcc taaagaggct tcgacgagca gcgtaacttt gacttcacgc cgaagatgta
     7441 tttgctgcaa ggccggaaaa acgatcgagc tgctgaggca gccgagaacg gcgccgatca
     7501 gcaacgcgtt gagccaggaa agatgcgccg cgaaacggca aacggtggcg acagctccca
     7561 tgcacaggag gtagctgaag agtgaaagga agaaaccaga gccgaagtgg cggaggattt
     7621 cgcgaagctt taattccaga ccgccttcaa aaaggatgag aatcagcgcg agagtgccca
     7681 agccgtgcgt ggcgccgcgg aaaacgtcgg gattgaccca gtggaagact gggccgatca
     7741 ggacgcccgt ggccataaga atgacgatgt ctggaacgcg agtgatgcgc acaagacggt
     7801 tggcaacaaa ggcgagaacg agaaggccac caagaattgc aaagaaaaga gtgaggtcca
     7861 ttgaggtggt ggattctgag aaagatcatg gaggaaatca gaggctcgcg caacgagaaa
     7921 tcagcgactc cggaggctgg aggcgttctg gaggctcgac agtagacgct agaagctcga
     7981 aagggcaaga agccggccac gcaaaacgac gcgtgagata gaagaaaaaa actacccgtt
     8041 ctggaggcca gaagctgcga actggtgacg gcaaagaaaa cgtacgcgta agaacgacgc
     8101 gcgggaggag tggagccggc agtttcggaa acgccgatgg tcgggcatga tcggcccgct
     8161 ttgaggtccg tcacagggcc gcgtgggcgt actccgagga tacaagacaa attttgaatt
     8221 aaaggcgtgg aatgatgcaa gtagttgttg gtgtaaagaa aaatcgtcca cggtgagtgt
     8281 ggtgaatggc acgatttcgg gttttttgta tcgcccccgc cgccagctta gccgccagag
     8341 tgtgacgccc aaccatcgaa cgctaggttc taatctgagc ggggcagtcc gcttctacat
     8401 ccatttgtcg atcgatgcgc tcttgacgtg gctcatgttc cccacctgga aaaaagcgtt
     8461 ggtttggccg ccgcaaacga cgaagttaat agaggtggct ttggggaacc acgggatcaa
     8521 cgtgtcctca ggagccttcc accaagtcgc atagggctct ctcccttcca acgcgagcgg
     8581 atatgtgtac acgtaggata gggaggactc ccggtattcc ttcgccgtgc gcatcgtatt
     8641 cttccacaac cactcggaga gcttttcctt ggtgccatat ccctgagcag ccaggcgctt
     8701 cgccaccagc ggatcgcaaa tcaccagcgc gccgaacgtt cccgccatcg tgtggaacat
     8761 gctgctgatt tgttcgtcga acatcggatc cgtgatggct cccgcgcccg tcgctccatg
     8821 accctggcgg atgtcccatc cgaggaacaa gctcaccacg ttctcctcgg gcttgaatcc
     8881 ctgttgcacg tgaaacggag tccacgggct gctcttctcg tcctctgcaa taatgcaatt
     8941 gttgtagtcc aactggttgc cctgcgaacc ttgataggtg tcgccgggta tgccgccaga
     9001 ggtgaggttc ttgcccatca acgtccatga tcgcccgatg gtggcattgg cctgcgcgaa
     9061 cgggctcagt gcgccgatcc cgtaattcat gttgagcttg tcgcggatgg ggccgttgat
     9121 cacgatcgcg ctgccgcccg acgtggtgga accaaaaaac cctaactggc ccgccgacgc
     9181 caatgcaagc actaccggaa aatattccgg ccggcatccc gccatcactg cgttcaccgc
     9241 cacgtgcctc actgtgaatc cccactgcgc ggactcatag cccacaccct ggatcgcttt
     9301 cttgagtact tcgtcggggt ggtgactcgt ccctttgagc atcgcgtcta ctttttcttc
     9361 ggtgggaatg atgaccggca tgtaatctgt cattccattg ttcatgaaga tttcttgcag
     9421 gctatccaag gtatccggac cgtaaacggc cggtcctgct gaaacattct caatcccggt
     9481 ctttttctct ccctcggaaa ggggcgtggt gaggttgcct atgatttccg tcatcattgg
     9541 ttttcccgtc acaggatcgg gacctgctac cattgccgcc agttctgcag gggtcttgcc
     9601 ccacacagga tgcggagtga acacgattgg tttaagcggc atgccccgct ttgctgcggt
     9661 ggttttggac aactccacga aggccctggt gcacaggggg acagcgggga ctcccatctt
     9721 ttccatcgtt atgcagtgac ccacggtcgc tggcgcgcaa ccccctcaat gcccaatggc
     9781 catgattgct gcgttgcctt ttgccttgat ttccgcccag agcggcgggt catccgcggc
     9841 ccacccgccg gccttcttgc gaaagacggt cttgacgctg ggcatattct gctgaaacca
     9901 cccctgcatt tctttcagca gcaagccgcc gccaaagaaa cctgtgtcga ctaagtagac
     9961 ggtctttcca tccagcgtgt ccagccgagg agccatggaa tagagctgca cagctggcgg
    10021 aatgcccttg ggactgacgg ccgtgaactt cgcttcgctg ccaacaccgc tatgaacgac
    10081 cttttttacc gcctgcgcct ccgccgccga gccagccaga agcaccggag aggtacccag
    10141 caacgacaat agctttcttc tattaattct catgaatgga ctacctccta ttctgcgatc
    10201 gcgcccctgc aaattcgggg cgcgcacatc cacaaccttc aaagcgggaa agtttgtctc
    10261 gattcagttc gctgcttggg aaacaccgaa ggtgctttgg gatgaaagtt caggtgaagc
    10321 ccacgaggga actcagtaga ttcctgtgcg aggtgaaacg catatggccc gaatccattt
    10381 agttttttct ccgccgactt ggaccagcca gcgactcctg attgctggac gcaaggcgga
    10441 ggaacagcgt ttcttcgaac tgcgtcggaa acccagcgga gcgtactgcg ccacgctccg
    10501 ctggacggct aaaccgatac tcagaagata tacttgatcg cgaactgcat gattctcgga
    10561 gcaacgtcgg attgaacctt gccgaacacg ctgctgttca gaaccgtgcc attggttccc
    10621 cccgcggcaa tgcccgaagt acccggattc aggaagttag tgtgatttgc aacgttgaag
    10681 gtttcccagc gaatctctat gccttgcctc tccgtgattg ggaatcggcg cgacagagaa
    10741 agatccacat cgacgtagcc agggccttcc aagctattcg ctctcgagtt tccgagggta
    10801 cccagcgccg gctgagcgaa cgcgctcgga ttaagccagc agttgttggc aagaaccacc
    10861 gagacgtgcc tgggagtgca gtaagggtct gagagcactt gattcggacg ttgtgcggga
    10921 atccccgtca gcgcatcgtc aatgccggtg gtcactgtga agtagctacc agtgtgtatc
    10981 cccacgatgg gcgcaagctg ccagtctccg gcgatcaact gcagcgctcg agacgaaaaa
    11041 cgcggcgact gaagaacacc cgaaaggttg aagttgtggt gaatgtcaat aaacgggcag
    11101 tttccccggt caaaccggcg gtctcccggc ttggtgccaa ttgggccgga aagctctgtg
    11161 gtctgcgggt ccgcgatgca atgcgaatat gtgtaattaa ccagcaccga aaaatggctt
    11221 gagaggtggt gttccgccga aacgatcagc gcgttgtagc tggctgtccc gtggtcgtcc
    11281 aagctctcag atgctccata gaatggccct tgactcggat tcaactgcgt caacaggcgc
    11341 cgcgcctggg tgttcctggt ggttgagcac ggtcccggcg ccgccagccc aaattggcct
    11401 gctacgcaat tgccggggac gaagactgct ggattcaact cctgatcgag ccaaaggtgt
    11461 attgagttgt tgcccaagta tgccgctttc aagagccagc ttctacccat ctgcttttga
    11521 agcgtcaggt tccactgttc cacgtacgtg ttcctgacgt gcagtggaac cgtttcgtaa
    11581 tatccaccta gcgggtaagc agaggcggca ttctgggtgg tcgggaacgg gcttccgcca
    11641 ggaaaaccaa gccagggatt cgcgaatcct cccgagggac tggtgatcgt gatgctctcg
    11701 ccccacagcg gttccgagga gtagttgaag tagaagagcg tgtgaggcat atcgtagaag
    11761 attccccaag acgcccgaat tagcgttttt ccgctgccgg tgggatccca tgccaagccg
    11821 atccgaggcg cccaattgtt ccaggccgta aagtttggcc gcccgccggt tcggaagccc
    11881 gcatcgcctg gataaagagt tcctgcggga gcgttcggga aaattgaaga gtgaacattg
    11941 ttgagaaagt ccgccattac gaaatgggtc acttggttgg gccgaagttg gccggcgaga
    12001 taaggctccc agcgcaggcc gtagttcagc gtcaggcggg aattgatctt ccaactatcc
    12061 tgcgcatata tccccaggga cctccagtac tcgttgtcat gcgtgagatt cagctgggtg
    12121 aacgaactga gctccccgga gataaaatcc gacatggcat tgccggtatt cgagcccgag
    12181 aacgtaaaca gcccgttgca gaaaacgcaa aaagtggaag tttgccacgg acgaataaaa
    12241 ctgaccccga actgcagctg atgggaaccc ttggtcaggc tgaagtcatc cttcaactgg
    12301 taagtcttgg tgttgacgag accgggagtg gcgaatccac cggcgctctg gaaatcgccc
    12361 gtgatgttga tgttggaata atggggcaga gctatgaaca tgccctggac tccgatgtcg
    12421 gtgggggtga caatcggcac ctgaaacttc gtgacgccgt ctgtattgaa cgcgaagtgg
    12481 aatgtgttca gcgcgttggg gctgactaga tacgtatgac ccaccaccgc cgaagtgaca
    12541 gtgtcgtccg tgcccgcatt ctggaccgat aattcgcttc cagtgaaact ggatggctgc
    12601 agactgtgag tgccaaagaa acgcacgaat aacgactgtt tggagttgat ctggtaatcg
    12661 actttcgcta cgcccatgtg ctcgttttga ttttgaataa ttcgatacgt aacgttgccg
    12721 cagggctccg gtcccacatc aaaatggctg gcgaaggcga gcgcttgcgg actgaccgga
    12781 gttgagagca cattattcac aaaaggagcc ttcaacgtct ggggtgtcgc gaaacaattc
    12841 gcttcatagg ccctgaagtc gccggtaagc tgcgatgcgg tgggaagaac ggcaggaatg
    12901 gctgcgggcg ccgatcgaat cagcgtgccc tgataaccca agaaaaagaa cagtttgttt
    12961 ttcttgaccg ggcctcccaa ggtgccgccg aactgattgc gttttaggtt gtcgctggcc
    13021 ccatttggat cactaggaga gaaggtgaag aaatcccttg cattgaaatc cgcgttgcgg
    13081 acgaattcga acgcgtcgcc atgaagctcg ttggtaccgg acttgctggc catgttgatt
    13141 gccccgcccg aatgctgtcc gtactcggcc ggcagcgcgc tggtctgcac gttgaattcc
    13201 tgtatggcat cgggaaaagg cgtgggcaag ttgaggttat tgaagggatc gttatacgtt
    13261 cccccatcca gcaggtaggt ggttccattc agcagtccac ccccaacgct taccaacgcc
    13321 tcgttcgggt agttcttgct ggagacaaga tttcctacgc tcggcgactg gccaaagccg
    13381 tactgcacgg cattggacgc gccagccagc gtgatcaact gcgttacttg gcgtccgttg
    13441 agcggcagat ccacgacgct ctgcgagttg accacctgac ccacgccggt agaggaagtt
    13501 tcgatcatcg ctgccgcagc ttccacctgc acggtttggc taacggcgcc tacattcaat
    13561 accggatcga tggtcgggcc ctgggcgact tgcagaacga tgcccgactg aaggtacgtg
    13621 gtaaagccat ccttcttcac ctccatttgg tagggaccga gtggcaggct cggcagcgaa
    13681 tataccccgt tcgcatccga cgtcgcggtc cgagtgactc ccgtagcggt ttgtgtgacg
    13741 gtaatctgag ctcctgctat cacccctccg ctggcgtcct taaccgttcc ggaaatctgc
    13801 gatgtagcct gcgcgaagac tcctgcgcaa tatcccaaac acaacgcact cactatgact
    13861 agttgtgcca agatccgctt catgtcccct cccaaagtct ggattcagat gactgaccgt
    13921 aattgaacag gtgaagagga agctgtggaa ggtatgccac accataccag aatcctatga
    13981 tgatacctgg caccttcccg cccatccacc gcaccaccgc ctgtttcccc ttactaccta
    14041 agcccgagtg ttgtcaagtt ctaattgaca gcgtccgacg caaaacgacg gattgctaag
    14101 cccaaagcaa acaaatatgc cacttttcca gaggctggcc gaactcagtg gccgaggctt
    14161 ataggccaag ggcgcgtgat cagcagcgtc atcaagagga agcccagaat ggcgacattc
    14221 ttttcgaaat tcaccaggtc gttcatcttg gcttgcgggt cttggaccgc ccagaaatta
    14281 tggatcatga gcgtggtggg cactaagaaa atcgccagca aaatcacgcc tatggtgggg
    14341 tgaaaaccga ggagcaggct cagaccgccg agcaggagca agattccgct ggttcccacc
    14401 gcgagctccg gcgccggagt gcccttcatt ttggcgtatc cggccatcat tttcaggcgg
    14461 gcaaaatggt tgacgccggc aatgatgaag aatccgccga ccaaaatccg gccaatcacg
    14521 aacgcgagaa cgtagatgcc gccgatgttg cccacaaatc ctcctcttgt gtgatgcggc
    14581 tcgcttccgc catttatgtt tgccccgagg agcgccgcag ggacggcaca cttatacacc
    14641 aaagggcttt tcaaaagata cttcggaagc ataaagggcc gacggttgag cttcgatcgc
    14701 cttgaagggc tcaacggcgc gaagatatcg aaacgaggtt tcgcagcgtc gttatatact
    14761 tcaacggaat catgcccaca atccggggat ggtgccgagg ggcagagttg aactgcctac
    14821 gccggccttt tcagggccgc gctctaccgg tgagctacct cggcaccgga acaattaagg
    14881 attctacgga aaacacgcgg cgctgaaaag cgaaaacacc ccgtccgcgt cgggcgagat
    14941 tgccccggaa ccgcaaagct tgcgcgggtg gcgaacgtcg aagcatcgga atacaattca
    15001 aaagcagctt gaaaagatga ccgagaagaa aatacgcgtg ttgatcgcca agcccgggct
    15061 cgatggccac gaccggggcg ccaaagtgat cgcgcgtgcg ctgcgcgatg ccggaatgga
    15121 agtgatctat acggggctgc gacaaacgcc ggagatgatt gcgtccgccg ccgtgcagga
    15181 agatgtggac gtgatcgggc tttcgattct ttccggcgcg cataatacga tttgcccgcg
    15241 cctaatggac ttattgcgtg ccaagggaat gaacgacgta attgtattga tcggcggcat
    15301 cattccggat actgatattc ccgcgctgaa gcaggccgga atcgctgagg tttttcttcc
    15361 cggcaccacc acgcagtcca tcatcgactt catccgcgag cgcgcggcgg cgaggcaggg
    15421 aatctaacgt gcctccgcga agggaagcta gcggcgaacc cgtacccgtt gccggaaccg
    15481 tgattcaaac gcgcgtggat cctaaagcgc cgccgtttca gaagaacagc cgcgacatga
    15541 ttgaccgcgt cgcggaaatc aaaaacgagg aagaaaagat tcgcaagggc ggcggcgcca
    15601 agtccatcga ggcgcagcac aaaaaaggcc gtttgaccgc tcgcgagcga attgcaaggc
    15661 tcgtggatcc gggaacgctt ttttttgagc tgggaattta cgccgccttc gaaatgtatg
    15721 cggaatgggg tggcgcaccg gccgcgggaa ccatcacagg actgggaaac gttgcgggac
    15781 ggcgcgtgat gatcatcgcc aatgacgcga cggtgaaggc cggcgctttt ttcccgatga
    15841 cggcgaaaaa agtgatccgg gcgcaaaata tcgccatcga aaatcgcgtt ccgacaattt
    15901 atctggttga ttcgggaggc gtgtttctgc cgcttcaaga agatgttttc cccgacaccg
    15961 acgatttcgg tcgcgtcttc cgcaacaatg cggtgatgag tgcgatgggc attccccaga
    16021 tcgccgcgat catgggactg tgcgtggcgg gcggcgccta cctgccggta atgtgcgatc
    16081 acgttctgat gaccgagggc agcggacttt ttctcgcggg acccgcgctg gtgcaaacgg
    16141 ccatcgggca gaaagtttcg ctcgaggaac tcggcggcgc acgagtgcac gcgcagatta
    16201 gcggtacggc cgactttcgc gaaccaaacg acgaagcgtg catcgaacgc gtacgcgctt
    16261 tagtcgacaa gatggggcat tcgcctggag cgcctttcga tcatatacgg ccggagccgc
    16321 ccgcatatcc gccggaggaa atttacggca ttttttccgc cgacccttcg aaacaatacg
    16381 acacgcgcga aatcgtcgca cgaatcgttg acggaagcag gttcgaggag tatcgcgcgg
    16441 agcgagggca gacggtcctg tgcggctatg cacgcgtcgg cggctgggcc gtgggaatcg
    16501 tggcgaatca gaaaaagaat gcgacaatct cggctcccgg aaccgatgaa aagcgaatcg
    16561 aatttggcgg cgtgatttat acggagtcgg ccgacaaagc cgcgcgcttc attctggatt
    16621 gcaatcagaa tctggtgccg ctgatttttt tgcatgacgt aaatggtttt atggtcggca
    16681 aagaggccga gtggactggc atcattcgcg cgggcgcaaa gatggtcaac gcggttgcga
    16741 acagcgtggt gcccaagatc acggtgatct gtggcggcac cttcggcgcc gggcattatg
    16801 cgatgtgcgg caaggcctac gatccacggt ttgttttcgc c
//
LOCUS       DATNDT010000496         6732 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_141520, whole genome shotgun sequence.
ACCESSION   DATNDT010000496 DATNDT010000000
VERSION     DATNDT010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141520"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1533
                     /locus_tag="VN788_16300"
     CDS             <1..1533
                     /locus_tag="VN788_16300"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HXU22145.1"
                     /translation="QGLYQTFFNFFGLRENAFRANPDPRYLFFSSTIRRALNELTVGS
                     QIGKSLIVLTGEAGTGKTSLVNHLLASLRKQEIPVSFVFNSHLDINNLFEFILSNFGV
                     KVDPKQNPNLRMVLNQWLAQHFQLGRAPVIILDEAQGLSTAVLEEVRMLLNMEVGGES
                     LVRIVLAGQPELDVTLHRPELRQLRQRITVRCRTAPLSLEETHGYIQHRLRVAGSPDG
                     AAFSRPALDAIYFYSGGVPRVMNLICEHSLINAYSDGVRPVPPEIVEDVAREFRFDDF
                     KPLPPRFTDRPVAEVIPMDSSAPRMRMSALQARESAPIEGPMVDAGLVAELMRPGASA
                     DRSVPAASPAVHRMAVAANASPPKIVVEPSVSARPIQPLTIMNPPPSLEVLAGPPAIA
                     SAAPSLEPPTPLPTIVNSAPPSDSPMRPHTPPPQTWDMPRAVTNMSWSRRAPASPIKL
                     RLQATSARLEASVTQIVHKLDELKALERLQRFAGSSIQWLRQPMRPMHSHRTSATPRQ
                     KR"
     gene            complement(1566..2510)
                     /locus_tag="VN788_16305"
     CDS             complement(1566..2510)
                     /locus_tag="VN788_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011343979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HXU22146.1"
                     /translation="MRYLVTGGAGFIGSHVVDELVRRGQDVAVLDDLSTGQEANIEGV
                     RKKIDFRVGTITDMKDVQSACRGADYVIHLAARASVPKSVKDPIETNHTNIDGTLNVL
                     VAARDAAARRFVFAASSSAYGEPPEQPKIETMAPAPISPYGVTKYVGELYAQVFGRVY
                     GLENTSIRYFNVFGPRQDPTSQYSGVLSRFMLAILRDERPVIYGDGEQSRDFTYVANV
                     VDETLRACEAAGASGRVFNGGTGARITLNQVINLLAKITGREIHAAYEPSRVGDIRDS
                     QADISLARKVLGYEPGVLFEEGLRLTWEWYNRAYGGNQ"
     gene            2668..3528
                     /locus_tag="VN788_16310"
     CDS             2668..3528
                     /locus_tag="VN788_16310"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HXU22147.1"
                     /translation="MIFLRARPRLSSQFGIVAIAIIAAGLWLGLELWPSSGAAFAAAG
                     GSQSARRRTSKPKHAPEKANEPAMPFRVGEILSYRVAWAGFSNAASLQLSVPERRDLY
                     GFQTWHFRVVTHTVSPVRRLFALDDQFDSYTDAATLESRQFETHLSEMGKIDDQVLHF
                     SASGQPPHSPGPVVVVSPGTRDPLGVLYALRAQNWQHAPDLRTPVYDGRDLFDMRARC
                     EALSEPVKVAAGNFSAARIAIHVFQYEKELSSIRFEIWIANDPARTPVVIEANLPFGS
                     VRAELLTMPK"
     gene            complement(3558..3938)
                     /locus_tag="VN788_16315"
     CDS             complement(3558..3938)
                     /locus_tag="VN788_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU22148.1"
                     /translation="MAGPTSTAADKQQLMLDTGRRRSMRVLLSVPIHVSGKTKTNQDF
                     DEETRTLVVNAHGALISLAAKVVAGQDVTLSNSSTQSSIGCRIVYLGNPTSGKVQMGI
                     EFLSPCPSFWQIDFPPDDWVVPEN"
     gene            4215..4553
                     /locus_tag="VN788_16320"
     CDS             4215..4553
                     /locus_tag="VN788_16320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HXU22149.1"
                     /translation="MTKLEAVIQTSKLDAVKDALHEIGVEGMTVLEARGHGRQKGHTE
                     VYRGREYTVDLIPKIKLELVLPDDLVEKTVQTIVAAARTGKIGDGKIFLTKVDEAIRI
                     RNDERGDSAL"
     gene            complement(4619..6028)
                     /locus_tag="VN788_16325"
     CDS             complement(4619..6028)
                     /locus_tag="VN788_16325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU22150.1"
                     /translation="MKRRSLRFRMMLLFCLVVGGFLLSTTAIIYSIFARELRLQIDRR
                     LSEAGVPMVADLAANPPDEDVFRLDLPDEYLELLDATSGTPLNMSQNWRAHPIDVGPL
                     DFPDGKPIFRTVEGAKGTLRMELIPFRLVGRPVVLAIAGPTRDIDIVLNNFRRVLIVL
                     LPVSLLLMGLISGWFVGRSLSPISDLTQHAARLAQLISEPDFVDMKAPRLVSHSHDEL
                     GVLAATFNELFGRVIAVLRQLRQFVSDASHEMRTPLSVLQGETELVLSERREPEEYER
                     ALEVIHDELRHLSRIVEGLFTLSMADAGQLRITHRELYINEVLEEACEIAIPLGRSKG
                     IRIEQYLEPEIVTTGDEAFLRDLFLVFLENAVKYSPANSVIRVSLAQVNDTVEVRFED
                     QGIGISPEHLPHIFERFYRATDTAEVEARSGGLGLAIAQAIVHAHGGRIECQTAQGQG
                     STFRVFLPAADTLISAPGS"
     gene            complement(6032..>6732)
                     /locus_tag="VN788_16330"
     CDS             complement(6032..>6732)
                     /locus_tag="VN788_16330"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU22151.1"
                     /translation="GKPREATMRLLLVEDERKVASFIARSLRENLYTVDVADTGEKAL
                     EMVSRATYDLILLDLRLPKLSGVEVCREIRDSGIETPVLMLTARSLVEQRVEGLDAGA
                     DDYLTKPFALAELHARVRALTRRGFHRGGAKLHAADLELDRPRRTVKRGDEDVPLTFK
                     EFALLELLMMRSPDPVLRSEIIEHVWSHGFDTETNIVEVYINRLRQKIDQQHAVKLIQ
                     TVRGSGYKLAPPER"
BASE COUNT         1402 a         2031 c         1888 g         1411 t
ORIGIN      
        1 caggggcttt atcagacctt tttcaatttt ttcggcttgc gtgaaaacgc tttcagagcc
       61 aatccggatc cccgctatct atttttcagc tcgactatcc gtcgagcgct gaatgaactt
      121 actgttggat cccagatcgg caagagcctg attgtattaa cgggtgaagc gggaacgggc
      181 aagaccagcc tggtcaatca cttactcgca tcgctgcgga agcaggaaat acccgtctca
      241 ttcgttttta actcccacct ggatatcaac aaccttttcg aatttattct gtccaatttc
      301 ggcgtcaagg ttgaccccaa acagaatccc aacctgcgca tggtgctgaa tcaatggttg
      361 gcccagcact ttcagttggg ccgggcaccc gtcattattc tggatgaagc ccagggcctc
      421 tccacggctg ttctagaaga agtccgcatg cttttgaaca tggaagttgg aggcgaaagt
      481 ttggtgcgga ttgtgcttgc agggcagccg gaactggacg tgacgctcca tcggcccgag
      541 cttcgccaat tgcggcagag aatcacagtg cgctgtagaa cggcgccact ttcactggaa
      601 gagacgcacg gatacatcca gcaccgcctt cgagttgcgg gaagcccgga tggcgctgct
      661 ttcagccggc cagccctgga tgcgatctat ttttattcgg gcggcgttcc gcgcgtgatg
      721 aatcttatct gtgagcactc actgatcaac gcgtattcgg acggcgtgcg tccggtgccg
      781 ccggagattg tggaggacgt cgcccgggaa tttcgctttg acgatttcaa gccgcttccc
      841 ccgcgtttca ccgaccggcc cgtggccgag gtgatcccca tggactcgag cgcgccccgg
      901 atgcgcatgt ctgccctgca agcgcgcgaa tcggctccaa ttgagggacc tatggttgac
      961 gccgggcttg tcgcggaatt gatgaggccg ggcgcgtccg ctgacagatc ggtgccggcg
     1021 gcttcacccg cggtgcaccg gatggcagtc gctgccaacg cttcgccgcc aaaaatagtt
     1081 gtcgagccaa gtgtatcggc gcgaccgata caaccactca ccatcatgaa ccctcctcct
     1141 tcattggagg tattggcagg gcctccagct atcgcgagtg ccgcaccttc actggagccg
     1201 ccgacgccac tgcctaccat tgtgaattcc gcgcctccat cggactcgcc aatgcggccg
     1261 catactccac cgccgcagac gtgggacatg cccagggccg ttaccaatat gtcctggagc
     1321 agaagagcgc cggcgagccc gattaagttg cgactccagg caacgagcgc acggcttgaa
     1381 gcgtctgtca cgcaaatagt gcataaactc gacgaactga aagcgctcga gaggctgcag
     1441 cgttttgcgg gaagttcaat tcagtggttg cgacagccga tgcggccgat gcattcgcat
     1501 cgtacgtcgg ctacaccgag gcagaagcgc tgagggttac ggcgctgaaa attttcggtc
     1561 gctgcttact ggttgccgcc gtacgcgcgg ttgtaccatt cccaggtgag ccttaatcct
     1621 tcttcaaaga gcactccagg ttcgtaaccg agcaccttcc tagccaagga aatatcggct
     1681 tgcgaatcgc gaatatcgcc cacgcgcgag ggctcgtagg ctgcgtgaat ctccctgcca
     1741 gtgattttgg ccagaaggtt tatcacttgg tttagcgtga ttcgcgctcc ggtgccgccg
     1801 ttaaagaccc ttccggaagc tccggctgcc tcacaggctc gcaaagtctc gtccaccaca
     1861 tttgcgacat aggtaaaatc gcgtgattgc tcgccgtcgc cgtaaatcac ggggcgttca
     1921 tcccggagaa ttgcgagcat gaagcgcgaa agcactccgg aatactgtga ggtcggatcc
     1981 tggcgcggtc caaaaacatt gaaatagcgg atggacgtgt tttccagtcc gtagacccgg
     2041 ccaaaaacct gcgcgtagag ttcgcccaca tatttggtca cgccgtatgg agaaataggg
     2101 gctggagcca tggtttcgat tttcggctgc tcggggggtt cgccataagc ggacgacgac
     2161 gccgcgaaca caaagcgtcg cgctgccgca tcgcgcgcgg ctaccagcac attcagggtg
     2221 ccgtcgatat tcgtgtggtt cgtctcgatg ggatctttca cggatttggg aacggaggct
     2281 cgcgctgcga ggtggatcac gtaatctgct ccgcggcatg cggattgaac atccttcatg
     2341 tcggtgatcg tgcccacgcg aaaatcgatt ttcttgcgga caccttcgat gttcgcctcc
     2401 tggccggtgg aaagatcgtc cagaacagcc acatcttggc ctcggcggac tagttcatcg
     2461 accacatggg aaccaatgaa ccccgcgccg cctgtaacca gatagcgcat cgcagaaact
     2521 ccagggtgag gtgactatat cggcttagat acggaaatgt gcgtgtaacg gatacgtccc
     2581 agtataaccg gcgtgttatg gtgatcaaga attgaagatt gatcgcgaca tgaaaactac
     2641 ggaacacaac cttttggact gcaggcgatg atatttttgc gagcgcgacc gcgattaagt
     2701 tcccaatttg gaatcgtcgc tattgccata atcgccgccg gcctttggct tggtcttgag
     2761 ctgtggccgt caagcggtgc ggcgttcgct gctgccggtg gctcgcaatc cgcacggcgc
     2821 cggacctcga agccgaagca cgctccggaa aaggcgaacg agccggccat gccgtttcgc
     2881 gtcggcgaga ttctgagtta ccgcgtggca tgggccggct tctcaaacgc cgcatccctg
     2941 caactcagcg tgccggagcg tcgcgatctc tacggtttcc agacttggca tttccgcgtg
     3001 gtcactcaca ctgtgagccc ggtccgaaga cttttcgcat tggacgatca gtttgactcc
     3061 tacacggacg cagccacgct cgaaagccgc caattcgaga cgcacctgag tgaaatgggg
     3121 aaaatcgatg atcaggtgct gcacttttcg gcaagcgggc agcccccgca ttcgcccggg
     3181 cccgtggtgg tcgtctcgcc gggcacgcgc gatccccttg gagtgctcta cgcactgcgc
     3241 gcgcagaatt ggcagcacgc tccggatctc cgcacgccgg tttacgatgg acgcgacctc
     3301 ttcgacatgc gcgcccgctg cgaggctctc agcgaacccg tgaaagtagc agccggcaat
     3361 ttttcagcgg cgcgcatcgc cattcatgtt tttcaatacg aaaaggagct ctcttcaata
     3421 cgcttcgaaa tttggattgc caatgatccg gctcgcacgc ccgtcgtgat agaggccaat
     3481 ttgccttttg ggagtgttcg cgcggagcta ctcacgatgc caaaataggt gcggatgttc
     3541 gcgcgcctgg caagaagtta gttctcggga acgacccagt catccggcgg aaagtcgatt
     3601 tgccagaacg aagggcacgg gctcaggaat tcgatcccca tctgcacctt cccggacgtg
     3661 gggtttccga ggtagacgat gcggcacccg atcgagcttt gcgtcgagct gttcgagagg
     3721 gtcacgtctt ggccggccac aacctttgcg gccagcgaaa tcaaagcccc atgggcattc
     3781 accaccaggg tgcgagtctc ttcgtcaaaa tcctgatttg ttttcgtctt gccgctcacg
     3841 tggatgggca cgctgaggag tactcgcatg cttcggcgcc ggcctgtgtc gagcattaac
     3901 tgctgcttgt ccgccgcggt tgatgtgggg ccggccataa catttctagc ctagaacccc
     3961 cgtcggccca tcgcaatagg actaaggtcc tacaagtaaa cagcctgcct tgggtgcgga
     4021 atgtcgcgcc cggggtggcc cgccgcgtct cggttccaca catggatgtt tcattcaact
     4081 catttgtgta acgcccgaag ccccagcacg ccatcccgat tcccccgccc atacggtatt
     4141 gcgcgtgtat aatgcgcgac ggctttccgg gcattaccct ggcatgatcg ttacggacgc
     4201 agaggcgagg gaaaatgacc aagctcgaag cggtgattca aacatccaag ctggatgccg
     4261 tgaaggacgc tcttcatgag atcggcgtcg aaggcatgac cgtgctcgaa gctcgtggac
     4321 atggacggca gaaggggcac acggaggttt accgcggcag ggaatatacc gtcgatttga
     4381 ttcccaaaat caaactggaa ctggttctgc cggacgatct tgtggagaaa acggtgcaga
     4441 caatcgtggc tgccgcgcgc actggcaaga tcggcgatgg caagattttc ctcaccaagg
     4501 tggacgaggc catcaggatt cgcaatgatg agcgcggcga cagcgcgctc taagagcagt
     4561 ccacaccggc aggcgcagcc tcacggccaa aggtggctcg cgcgagtccg cgattccgct
     4621 atgagccggg cgcggaaatg agcgtgtccg ccgccggcag aaaaaccctg aaggtcgagc
     4681 cctggccctg agccgtttgg cattcaattc gcccgccgtg cgcgtgcacg atcgcttgcg
     4741 cgattgccag gcccaggccg ccgctgcgcg cttccacttc cgcagtgtcc gttgcgcggt
     4801 aaaaacgctc gaatatgtgc ggcaagtgct ccggcgagat tccgattccc tgatcttcaa
     4861 accgtacttc gacggtgtca ttcacctggg ccaggctcac ccgaatcacg ctatttgccg
     4921 gtgaatattt cacggcattc tctagaaaga ccaaaaacaa atcgcgcagg aaggcttcgt
     4981 cgccagtcgt aacaatttca ggctccagat actgctcgat tcggatgccc ttcgaccggc
     5041 ccagcggaat cgcgatctcg caggcttctt ccaaaacttc gtttatatag agttcccggt
     5101 gcgtgattcg aagctgaccc gcgtccgcca tggacagcgt gaatagccct tccacgatcc
     5161 ggctcagatg acgcagttcg tcgtgaatca cttccagggc gcgctcgtat tcttcgggct
     5221 cgcgccgctc ggagagcacc agctccgttt caccttgcag cacggagagc ggtgtccgca
     5281 tctcatgcga cgcatccgat acaaactgcc tcaattgccg aagcaccgcg atcacccggc
     5341 cgaagagctc gttgaacgtc gcggccagca cacccaattc gtcatgcgag tgcgatacaa
     5401 gcctcggcgc tttcatgtcc acgaagtccg gctcagaaat cagttgcgcc agtctcgcgg
     5461 cgtgctgcgt caaatccgag atcggcgaca agctgcgccc cacaaaccat cctgatatta
     5521 gccccatcag aagcaaacta accggcagca acacgatcaa cactcgccga aaattgttca
     5581 gcacgatatc gatatcacgg gtcggtcctg cgatagctag caccaccggg cggccaacca
     5641 atcggaaggg aatcagttcc attcgcaacg tgcctttcgc tccctccacc gtgcgaaata
     5701 tcggcttgcc atccggaaaa tcgagcggcc ccacgtcaat cggatgcgcc cgccagtttt
     5761 gcgacatgtt cagcggcgtc cccgacgttg catccagcag ctccaggtac tcatccggaa
     5821 ggtctaggcg aaaaacatct tcgtcgggag ggttcgccgc aaggtcggcg accatcggga
     5881 ctcccgcttc gctcagtctg cgatcgatct gtagccgaag ctcgcgcgca aagattgagt
     5941 agataatggc ggtggtgcta aggagaaatc ctcccaccac caggcagaag agaagcatca
     6001 tccgaaatcg aagcgaccgt cgcttcatcg ctcaccgttc cggcggagcc agcttgtatc
     6061 ccgacccccg caccgtctgg atcagcttca ccgcatgttg ctgatcgatt ttctggcgca
     6121 gccggttgat atacacctca acaatattcg tctcggtgtc gaagccgtgt gaccacacgt
     6181 gctcgataat ttccgatcgc agcaccgggt cgggagaacg catcatcagc agttccagca
     6241 gcgcaaattc cttgaaggtc agtggcacgt cttcgtcgcc gcgcttcacc gtcctgcgag
     6301 gacggtcgag ttccaggtcc gccgcatgaa gtttcgcgcc gcctctgtgg aatccgcgcc
     6361 gcgtcagtgc gcggactcgc gcgtgcaact cggccagcgc aaaaggtttg gtcagataat
     6421 cgtcggcgcc cgcgtccagg ccttccacgc gctgttccac caggctgcgg gccgtcagca
     6481 tcagcaccgg agtctcgatg cccgaatccc gaatctcgcg gcacacttcc actccgctca
     6541 gcttcggcaa acgcaaatca agcagaatta aatcgtaagt cgcgcggctc accatctcga
     6601 gcgccttttc gcccgtgtcc gccacatcca cggtgtacag attctcgcgc agcgaacgcg
     6661 cgataaagct agccactttt ctttcgtctt ctaccagaag aagacgcatg gtcgcctccc
     6721 tcggtttgcc cc
//
LOCUS       DATNDT010000497         4092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1857639, whole genome shotgun sequence.
ACCESSION   DATNDT010000497 DATNDT010000000
VERSION     DATNDT010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1857639"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..605
                     /locus_tag="VN788_16335"
     CDS             30..605
                     /locus_tag="VN788_16335"
                     /inference="COORDINATES: protein motif:HMM:NF013583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HXU22152.1"
                     /translation="MSDQQRTTNPKRAPAKEFQRRDRPQPAPPLDAEKAAGELKKFLD
                     LVAREMQLEIEYDISLAQSSERGARDSDVVVTFRGSDQELLLEHNGELLLALEHIGHR
                     WLRLDPRLHDRVRFDCGDYRATRLAELKLSARVAAQRVRETGDPFRFNPMSSRERRIV
                     HLELNGAPGVRTMSDGSGDRRQLVIYPVTKK"
     gene            complement(622..1227)
                     /locus_tag="VN788_16340"
     CDS             complement(622..1227)
                     /locus_tag="VN788_16340"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HXU22153.1"
                     /translation="MKILIFSDIHGDVRALERILAQPADLYIAAGDLATFGKGLERCG
                     EVLKPLGAKLWVLPGNHETHDDTRALCQRFGFVDFHRQIRSLHSSDGELHLAGLGYSN
                     ITPFNTPGEYSEEEISSALSAFDGIEPLYLVVHFPPAGTKLDEYAPGKHAGSPALREW
                     VQKARPPYLFCGHIHETAGLSDSLGNTQCINVGKQGYALEI"
     gene            complement(1224..2489)
                     /locus_tag="VN788_16345"
     CDS             complement(1224..2489)
                     /locus_tag="VN788_16345"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HXU22154.1"
                     /translation="MDPVTHGITGALLGKGYFSERYGRVAVFAATLGAVFPDVDVTAD
                     IFSRDPLAIVKYHRAITHSWVALPVFAFLLALLTRLAVPWVKKRSERFRDIESPPLAT
                     LTLIYGIGIASHIILDGMTSFGTRMWYPFSNRRVAWDLLFIIDLTFMAILLLPQMLAW
                     IYSDDRPDTSHDPGKSRRRAWRVWIWFTAGTLAAWLLTKAASYPFRLWVAALVSLILA
                     AIIFLPAMNRWGFRVTRARWCQAGTIVALAYLFSCAWAHHAALLRAKSFADHRHIEIR
                     RMGALPIPPSLLDWGDAVRSPDGIYEAQFDLRTPGPPDFRFVPDSPPDPFIARAFSLP
                     NVKLYWNFARFPSIDSFVQGRDHIIELGENRFAAGRRRSPQPFTYQVIFDAAGNLIQQ
                     GWLTNGMPGRRMRQMVPQPPTPQSPKRSP"
     gene            2623..3972
                     /locus_tag="VN788_16350"
     CDS             2623..3972
                     /locus_tag="VN788_16350"
                     /inference="COORDINATES: protein motif:HMM:NF012296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HXU22155.1"
                     /translation="MNTNGNRVPPGPKGLPLVGMALDLRRDPLEGMRRMVRDYGDIVH
                     FQVMGQDRILLNHPDLINQVLVIQQAKFHKSELTRRITGRMLGQGLLISEGDFWRRQR
                     RLVQPAFHRSRMAEYGATMRDLAQAHMRGWRDGEERNVGQEMMGLTLNIAVRTLFGTS
                     LPGEAHEVGQAMTFLMRYSLRRQRLPFRMPEKWPTPNNRRANRELEFIASIVYRIISE
                     RMAEGNSNHTDLLALLMGAMDEDGSQMTPQQLHDEAMTLFIAGHETTAQMLSWTWFAL
                     SGNPSAERKLHEELSSVLSGRSPEAGDFPRLPYLRAVMNEILRLYPPAYILARETVQP
                     CQIGGYELKLGSTVILSQWITHRDARYFDDPETFRPERWLDGLADRLPAGAYFPFGAG
                     PRRCVGEGFALLEAAIVTATLAQRFSFRLVPGYPVVAEPLVTLRPRQGIQMTLHARS"
BASE COUNT          911 a         1154 c         1233 g          794 t
ORIGIN      
        1 cgaagtaaat tcaaaaagaa aaaagaagca tgagcgacca acagcgaacc acgaatccga
       61 aaagagcgcc tgccaaggaa ttccagcgcc gggatcgccc gcaacccgcg ccgcccctgg
      121 acgcggagaa agccgcgggc gagctaaaaa agtttttgga tctggttgct cgggaaatgc
      181 agctcgagat cgaatacgac atctccttag cgcagagctc ggaacgaggt gctcgtgatt
      241 ctgacgtggt ggtgacgttt cggggctcgg atcaggaact gctgctggag cacaatgggg
      301 agttgttgct ggcgctcgag cacatcggcc atcgttggct tcggctcgac ccgcggctgc
      361 acgatcgggt acgatttgat tgtggggatt atcgcgccac gcgactggcg gaattgaaac
      421 tctccgcgcg cgtcgcggca cagcgggtac gcgagacggg cgaccccttt cgcttcaacc
      481 cgatgtcgtc acgggagcgc cggatcgtcc atttggaact caatggcgcc cccggcgttc
      541 gcacgatgag tgacggctcc ggcgaccggc ggcaactcgt gatttatcct gtaaccaaga
      601 aatgacgcgc gcaggccgcc tctaaatttc cagcgcgtat ccttgtttgc ccacgttaat
      661 gcactgcgtg tttccaagcg aatcgctgag gcccgccgtt tcgtgtatgt gcccgcagaa
      721 aagatacggc ggacgagcct tttgaaccca ttcgcggagc gccgggctgc ccgcatgttt
      781 cccaggcgcg tattcgtcga gcttggtgcc ggctggcggg aaatgaacca cgagataaag
      841 cggctcgatc ccatcgaacg cggaaagcgc gctcgaaatc tcttcttcgg agtattcacc
      901 gggcgtgttg aaaggtgtga tattgctgta gccgagcccc gccaaatgca attcaccgtc
      961 gctcgagtgg agcgaacgga tttggcggtg aaaatcgaca aaaccgaagc gctggcacaa
     1021 ggctcgcgtg tcgtcgtgcg tttcatgatt gcccgggagc acccagagct tcgcgcccaa
     1081 cggcttcaga acctcgccgc agcgttccaa ccctttacca aacgtggcga gatcgccggc
     1141 ggcaatatac aagtcggccg gctgcgcgag tatgcgttcg agcgcgcgaa catcgccgtg
     1201 aatatcgctg aaaatcagaa ttttcatgga gatcttttag gcgattgcgg cgtcggcggt
     1261 tgtggaacca tttgccgcat acggcggccg ggcatgccat tggtaagcca tccttgctgg
     1321 atcaaattcc ctgcagcgtc gaaaatcact tgataggtga agggctgcgg gcttcggcgc
     1381 cgtccggccg cgaagcgatt ttcgcccaac tcgattatgt gatctcttcc ctggacgaag
     1441 ctgtcgatcg acggaaaacg cgcgaaattc caatagagct tgacgttcgg aagtgagaag
     1501 gcgcgggcga taaaaggatc tggcggagaa tccgggacga atcgaaaatc gggaggccca
     1561 ggagttctca aatcgaactg cgcctcataa attccatccg gagagcgaac ggcgtcaccc
     1621 cagtccagca acgacggcgg aatcggcagt gcgcccatgc gacgaatctc aatatgccga
     1681 tgatcagcga acgatttggc gcggagaagc gctgcatggt gcgcccacgc gcaactgaaa
     1741 agataggcga gggcaactat ggtcccggcc tggcaccacc gggcgcgggt tactcggaat
     1801 ccccagcggt tcatcgccgg caggaaaatg atagccgcca gaataagact taccaaggct
     1861 gctacccaca agcggaaggg gtaactcgcc gcttttgtta gcagccaggc cgccaaagtg
     1921 cccgccgtaa accaaatcca tactcgccaa gcccgtcggc ggcttttgcc cggatcgtga
     1981 gaggtatcgg gtcgatcgtc ggaataaatc cacgcaagca tctgcggcag cagcaaaatt
     2041 gccatgaacg tgagatcgat aatgaacaac aggtcccatg ccacgcgtcg gttcgagaac
     2101 ggataccaca tgcgggtgcc gaacgatgtc attccgtcca gaatgatgtg gcttgctatg
     2161 ccgatgccat agatgagtgt gagagtcgcc agcggcggcg attcgatatc gcggaatcgc
     2221 tcgctgcgct ttttcaccca tggcacagcg aggcgcgtga gcaacgcgag gagaaacgcg
     2281 aaaacaggta gcgccaccca ggagtgggtg atggcgcgat ggtatttgac aatcgcgagt
     2341 ggatcgcgcg aaaagatgtc tgctgttacg tcaacgtccg gaaaaaccgc gcctagtgtt
     2401 gccgcgaata cggcgactcg cccataacgt tccgaaaaat atcccttgcc gagcagcgcg
     2461 ccggtgattc cgtgagttac cgggtccata aggaatcgaa accgaagcag tgtacacgat
     2521 tcaatgtgcg tacgcgatcg tggcgcaggc actcttgccc gcgcggataa gtcgaggcga
     2581 tcagttaagc agcacgctgc tagaatggcc agagccgcaa acatgaatac gaatggaaat
     2641 cgagttccgc ccgggccgaa gggcctccct ctcgtcggca tggctctgga cctgcgccgc
     2701 gatccgctcg agggaatgcg ccgcatggtg cgcgattacg gcgacattgt tcactttcag
     2761 gtgatggggc aagatcgcat tcttctgaat catcccgatt tgattaacca ggtgctcgtt
     2821 atccaacagg caaaatttca caaaagcgaa ttgaccagaa ggattaccgg aagaatgctt
     2881 ggccagggat tgctgatcag cgaaggagat ttctggaggc gacagcggcg cctggtccag
     2941 ccggctttcc atcgcagccg tatggccgaa tacggcgcca ccatgcgcga tctcgcgcag
     3001 gctcatatgc gcgggtggcg cgacggcgag gaacgcaacg tggggcagga aatgatgggc
     3061 cttactctga acatcgcggt gcgcactcta ttcggcactt cgcttcccgg agaggcccac
     3121 gaagtcggcc aggcgatgac tttcctgatg cgctattcgc tgcgacgcca aagattgccg
     3181 ttccggatgc cagaaaaatg gcccaccccc aataatcgtc gcgctaatcg cgagctcgag
     3241 ttcatcgctt cgattgtcta ccgcattatt tccgagcgca tggcggaagg aaattcaaat
     3301 cacactgatt tgcttgcgct gctgatggga gccatggacg aagacggcag ccagatgacg
     3361 ccgcaacaac tccacgatga agcgatgacg ctttttatcg ccggccatga gaccacggcg
     3421 cagatgttgt cttggacatg gttcgcgttg agcgggaatc cgtccgctga aaggaaattg
     3481 cacgaagaac tgagcagtgt gttgagcggg cgttcgccgg aagcagggga ctttccccgc
     3541 ctgccatact tgcgagccgt gatgaatgaa atcctgcgcc tctatccgcc agcctatatc
     3601 ctggcgcgcg agacggttca gccctgccaa atcggcggtt atgagttgaa actaggttca
     3661 accgtcattt tgtcgcaatg gatcacgcac cgcgacgcgc gctatttcga cgacccggag
     3721 acctttcgac ccgagcgatg gctcgacggc ctcgcagacc gtttaccggc tggagcctac
     3781 tttccatttg gggcagggcc gcgccgatgc gtcggcgagg gatttgcgct tctcgaggcg
     3841 gcaatcgtga cggcaacgct tgcgcagcgt tttagttttc gattggttcc tggataccca
     3901 gtggtagcag aacctctggt gacgctgcga ccgcgtcagg gcatccagat gacactccac
     3961 gcgcgctcat gagcgacggg cgttcgccgc ggcggaatgt gcccggcaaa cttacttggc
     4021 gtgatctgcg ccttgcgcct cgcacaaacg ggcgccccct gtggagtgcg ggagatcgct
     4081 cccgctgtat gc
//
LOCUS       DATNDT010000498         2745 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1858573, whole genome shotgun sequence.
ACCESSION   DATNDT010000498 DATNDT010000000
VERSION     DATNDT010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2745
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1858573"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1422)
                     /locus_tag="VN788_16355"
     CDS             complement(<1..1422)
                     /locus_tag="VN788_16355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HXU22156.1"
                     /translation="MPDGPAAEQRRVIIEHVRPEIDGGRFPVKRVVGDTVIVEAAVFA
                     DGHDQVACRVLHWRQNPSDAHSSSMKPLGNDLWRGGFSVPALGRYFYTVEGWIDRFQT
                     WRGDLVKRIAAAQDIRTDLLVGAALIEESASRASAAAIHAKTLRAWSEKLRREPSDEC
                     AKMVVADEELLELMQRYPDWRSATRYSKQLVVVVDREKARFSTWYEVFPRSCSAEPGR
                     HGTLRDCEARLPYIASMGFDVVYLPPIHPIGETFRKGKNNSSPAGPDDVGSPWAIGSS
                     KGGHTAVHPKLGTLEDFDRLCRKAKDRGLEVALDIAFQCSPDHPYVQQHREWFRSRPD
                     GSIQYAENPPKKYEDIYPLDFETKDWRALWAELKSVFLFWIGRGVRIFRVDNPHTKPF
                     AFWEWVINEIKPEHPDVLFLAEAFTRPHVMYGLAKLGFSQSYTYFTWRNTKQELTEYF
                     TELTRTEVREYLRPNLWPNTPDIL"
     gene            complement(1704..>2745)
                     /locus_tag="VN788_16360"
     CDS             complement(1704..>2745)
                     /locus_tag="VN788_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU22157.1"
                     /translation="LLKNDIGIVLMDVSMPELDGFELAEMIRQHPRFQKTAIIFISGV
                     HLTDLDRIKGYQRGAVDYISVPVIPELLRAKVSVFAELHRKTRQLETLNSELRRLSSR
                     LITMQDDDRRRIARELHDGLGQDLAAAKMILDGIPPERQSPQINREISSEVSLLIDHA
                     IKQIRSMSYLLHPPLLDEVGLQSALQWYLEGFSKRSGIETVLSVEPLDLPRLPLELET
                     AIFRAIQEALTNAFRHSGAKKIWVNLLRTDPQIVGTVRDDGKGISDRVSKLQPDSIGI
                     GISGMIQRLKEFGGELRLKNSSPGTIVELTIPIDQTAANHGQPKTRPREAVDVVGGGI
                     LAGSAEVPQKLF"
BASE COUNT          633 a          807 c          699 g          606 t
ORIGIN      
        1 caagatgtcc ggcgtgttag gccacaaatt tggccgaaga tattctcgaa cttcggtgcg
       61 agtcagctcc gtaaaatact ccgtaagctc ctgcttggta ttgcgccacg taaaataggt
      121 gtaggactgc gaaaacccca atttggccag accgtacatc acatgcggcc tggtgaaggc
      181 ttctgccagg aacaacacgt ccggatgctc gggcttgatt tcgttaatca cccattccca
      241 gaaagcgaag ggcttggtat ggggattatc gacacgaaag atgcgcacgc ctcgcccgat
      301 ccagaagaga aaaaccgatt tcagctccgc ccagagagcg cgccagtcct tggtctcgaa
      361 atccaacgga tagatgtctt cgtacttctt tggcggattc tcggcatact gaatgctgcc
      421 atcgggtcga gaacggaacc actctcggtg ctgttgaacg taaggatgat ccggcgagca
      481 ctgaaacgca atatccagtg cgacctctag gccccgatcc tttgctttcc ggcacaggcg
      541 atcgaaatct tcgagcgtgc ccagcttggg atggaccgcg gtgtgcccac ctttcgaaga
      601 accgatagcc cacgggcttc ccacatcatc aggaccagcg ggagaagaat tgtttttacc
      661 cttgcgaaaa gtttcgccaa tcggatgaat cggcggaagg tagacaacat cgaaacccat
      721 cgatgcaatg tagggcagcc gggcctcgca atcgcgcagc gtgccatgac gcccgggttc
      781 agccgaacaa gacctcggga atacttcata ccacgttgag aagcgggctt tctctcgatc
      841 caccaccaca accaactgct tgctgtagcg cgtggcggag cgccaatccg gatatcgctg
      901 catcagttcg agcaattcct cgtccgcgac gaccattttt gcgcattcgt cgctcggctc
      961 tcgccgcaat ttctccgacc aagcgcggag cgtcttagca tgaatggctg ccgcacttgc
     1021 gcgagaagcg gactcttcaa tcagcgccgc gcccacgagc aaatccgtac gaatatcctg
     1081 cgcggccgca attcgtttga ccaaatcgcc gcgccaagtc tgaaaccgat cgatccagcc
     1141 ctcgaccgtg taaaaatatc ggcccaaagc ggggacggaa aatccgccac gccacaagtc
     1201 gttgccaagc ggcttcattg acgacgaatg cgcatcggac ggattttgtc gccagtgcaa
     1261 cacccggcac gcaacctggt cgtgaccatc ggcaaaaaca gcagcctcaa caataaccgt
     1321 gtctccgacc acccgcttca cggggaatcg tccgccgtcg atttccggac ggacgtgttc
     1381 aatgatcacg cgtcgctgtt cggcggccgg accatccggc atgtcgggtt cctcctacac
     1441 ctatcctagt gtgtgctcga ctctgagttc cagtggcttt tcaatggcgt gctgcggctg
     1501 gcaacgcgct actccgatga accgacgacc aatcgccgat gtggagtgcg gcaacacgcc
     1561 ctgagcaaac gcgaattaag aggtgctgac gaaaatcacc gtggacgatt tttctttata
     1621 ccaaccactt gcgggaaatg aggccccggt gtaaagatat tgttcgtatc ctcgtactgc
     1681 gtccattcac actgctggaa ggttcagaaa agcttctgag gaacttccgc cgatccagcg
     1741 aggattcctc cacccaccac gtcgaccgct tcacgaggtc tggttttcgg ctgtccgtga
     1801 tttgccgccg tctggtcgat agggatggtt agttccacaa ttgtcccagg cgatgaattt
     1861 ttcaaacgca actccccacc gaactccttg agccgttgaa tcatgcccga aatgccgatg
     1921 ccgatgctat ccggttgaag ctttgacaca cggtcggaaa taccctttcc gtcatctcgg
     1981 accgttccca cgatttgcgg gtcagttctt aataaattca cccatatctt cttggcgccg
     2041 gaatgtcgaa atgcatttgt aagggcctct tgaatcgcgc ggaatattgc ggtttccaat
     2101 tccagcggca accgcggaag atccaatggc tccacgctca gaactgtttc gatgccgctc
     2161 cgcttgctga atccctccaa ataccattga agagccgact gcaagcctac ctcgtccaat
     2221 aaaggcgggt gcaataggta tgacatgctg cgaatctgct taatcgcgtg atcgatcagc
     2281 aagctcactt cgctggaaat ctcccggttg atctgaggcg attgccgctc cggcggtatg
     2341 ccgtccaaaa tcattttggc ggccgcgaga tcctggccca aaccatcatg cagttcccgg
     2401 gcaattcgac gccgatcgtc atcttgcatg gtaatcagcc gtgaagaaag gcggcgcaat
     2461 tccgagttga gcgtttccaa ttgccgcgtt ttccgatgca gttccgcgaa aacgctcact
     2521 ttggcgcgca gcaactccgg aatcacgggg actgaaatat aatccaccgc gccgcgctga
     2581 taacccttga ttctatccag atcggtgaga tggacgccgg aaatgaaaat gatcgccgtc
     2641 ttttggaatc gcggatgctg tcggatcatt tccgccaact cgaaaccatc gagttccggc
     2701 atgctcacgt ccattagcac gattccaata tcgttcttca gcaaa
//
LOCUS       DATNDT010000499         6936 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1559472, whole genome shotgun sequence.
ACCESSION   DATNDT010000499 DATNDT010000000
VERSION     DATNDT010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1559472"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="VN788_16365"
     CDS             complement(<1..611)
                     /locus_tag="VN788_16365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22158.1"
                     /translation="MSFHRVRVLVKNLLAVAICCVIAGFAFCPLSKAQAAPSESHQHV
                     GWIPLAFLQRPIALQTGIGSIHEQVTTSSKQAQEFYDQGLAYLQSYFWAQAARSFYQA
                     LHYDPKLAMAYVGLSYAYSPMDFPAAKAALAHAKALSSGVTDRERRRIQIRALQLDAM
                     VDPSKIERELAVRDALDDALAAYPNDVVFLLLRGVAQEPSPFA"
     gene            complement(617..1693)
                     /locus_tag="VN788_16370"
     CDS             complement(617..1693)
                     /locus_tag="VN788_16370"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase"
                     /protein_id="HXU22159.1"
                     /translation="MPQQTQSADDRMIVYSRKYLTLEMPMSLLNSWITPVDTFFVRNN
                     LLMPATVDPSTWTLSITGEVERPQTINFHDFMQLPTSRVINTLECAGNSRVNFSPRIG
                     GVPWGRGAVGNAAFEGPILADVLRHAHLKPTARHVAFRGLDIVPQGASEFIRSIPTEK
                     AMERSTLLATKMNGSPLTPSHGFPVRALVPGWIGSASIKWLQEIRVLSHEFHGFYMDP
                     GYRIPVASAAQQSGHVKTMSLTSLRVKSIIAEPADGAALEPSQHGVTIRGASWAGESA
                     IARVEISTDGGRNWHAASLDPDHAKYAWRLWHYQWQPSRPGAYVILSRATDSTGRTQP
                     LEPRWNPGGYLWNGMDKIHVQIGK"
     gene            2139..5603
                     /locus_tag="VN788_16375"
     CDS             2139..5603
                     /locus_tag="VN788_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HXU22160.1"
                     /translation="MKRIAFYGALCTLLVCGLAGFAAAQTTGGTLRGAVTDSQGLAIA
                     DASVTITNQETKVTIPLSTTAAGLYNYPDLPVATYSITVEKDGFQKFIRNGIQIFANQ
                     VTEVNVVLPLGSVSSTIEVTGSTPLVQTGTSQISNDFTATQVTQLPNPDPSGSQLQLA
                     LLAPGATSQQAGVLGEGGSIGGTRPRMNSFTIDGVDDNRIDLTGHTQYVIPESVSEFN
                     LLTNQFAAEYGHSAGGQFNTITKSGTNDWHGSAWEYNNNRNYNAYDNLEKQNVDANGR
                     VLPKPRYDFNRAGTEIGGPIIHDKLFIYGAFARIIQGLATQAVVQHTVTAAGLTTLES
                     VSDPAVQDILKQFPTAGSSDGSELVTVPGTSCAGGCPIPTGPISPLAPNFFNQWDFIV
                     NADATLGKHSLSFHVLYDRNRQPNVNPDTPQAQFTGAISSDSRKYLFKDTWVISNRFV
                     NEFRTGYSRLVNSFGVPPAFSNFPNAEVDTLGINVGPQGCSPQSTILNTYQFADNMSY
                     VVGKHTLKWGVQWLHAIAPTNFLPRARGEWDYTNLNQLVNDFAPNGLNGALRGAGDPL
                     FRGNQNGASGFIQDDWRVTPRLTLNLGLRYEWNGIPLDDNLQNLNAISTLPGVFEFRT
                     PKADYNNIGPRVGFAYDPTGNGKWAIRAGFGISYDQTPQNFVTLSLPPQLQTEQAPQI
                     TCALAGAPAWCANFSAATYGQPGGNTGQGFLQGGGDLQVNVPCTTVSDCRTSTQALIV
                     DVVEPKVLTWSLGVQHEVGWNSSLEVRYVGTRSLELPIQARLNTQSGFAAGLGALPTY
                     LSSSDVPATVAAPSATLADWDNFENNAGGAATPNGCADPSPFKFGVQGFCGSLVTAFP
                     PLANGIYHGVSADFNHRVGHGLTLRANYTYSKNMDDATNELFSSRVNPRRPSDWEDLR
                     NDWARSTLDVPSKFTVSWVYDLPTSHSDNGFVRGFANGWQWEGSWQLYNGTPVTILNG
                     GDSNGNLDSAGDEPVLNPKASGNGVSGIDFVCNAGAGGAVTIVSDPTTCGAGDDSNIV
                     GYVAQDSTARYVSAGLGTRSTVPRNSFRSPGVNMWNMGFQKDTRITERVHLAFNVSAY
                     DVFNHRNYALAQPDVLEAANGSFINTVNNALSTTYTNINAAGSGFLDKTQFSGGSRKI
                     QLGVKLTF"
     gene            5809..>6936
                     /locus_tag="VN788_16380"
     CDS             5809..>6936
                     /locus_tag="VN788_16380"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU22161.1"
                     /translation="MQAKTSMTFGEAVRIATASLWSHKLRSMLTLLGVVIGVMSVIAV
                     VSLINGLNDYVAGAISSNLSADVFMISRAPSTITNVDQWESAQKRRKFHFDDYEYLRD
                     NCRSCVAVAANLNKIGQVKYGTDYLDDSAIIGFTYEMPQVYSRELAEGRDFTRLDVAR
                     SAAVCDVGYDIVNKLLPGVDPIGKVIHVDTGECEIIGVAKKRGSTMGQSQDNWVIMPL
                     TTYQGMYGTNDSLRLWVKAAGKNAIDPTMDEVKLLLRARRHVAYRDADDFAMETNASF
                     LSLWSSISSTFFGVTIGIASISLIVGGIVIMNIMLVSVTERTREIGLRKSLGARQSDI
                     RMQFLIESAALAAIGGACGVILGIIAAKIVSMTTALPSSVQI"
BASE COUNT         1563 a         1903 c         2017 g         1453 t
ORIGIN      
        1 tcggcaaagg gcgaaggctc ctgcgccacg ccgcggagga gaagaaacac gacgtcattg
       61 ggataagcgg caagcgcatc atccagagca tcgcggacgg ccaattcgcg ctcgattttg
      121 ctcggatcga ccatggcgtc cagttgtagc gcgcgaatct ggatccgccg gcgctcccta
      181 tcggtaacac cggaagagag cgctttggcg tgggcgaggg cggctttggc agcgggaaaa
      241 tccatcggcg agtaggcata actcaaacca acgtaggcca tcgccagctt cgggtcgtaa
      301 tgcagcgctt gataaaagga gcgagcggcc tgggcccaaa agtacgactg gagatatgcc
      361 aggccctggt cgtaaaactc ctgagcttgc tttgaagatg ttgtgacttg ctcgtgaatc
      421 gacccgatgc cggtttgcaa ggcaatggga cgctggagaa aagccagcgg aatccagcct
      481 acgtgttggt gagactcaga gggagcggcc tgagccttgg agagagggca gaaagcaaat
      541 cccgctatga cgcagcagat tgccacagcg aggaggttct tcaccaacac tctcactcta
      601 tggaaagaca ttcacattat tttcctatct gaacgtgaat cttgtccata ccgttccaga
      661 gataaccgcc tggattccag cgaggttcaa gcggctgggt gcggccggtg ctatcggtcg
      721 cgcgtgaaag aatcacgtag gcgccgggac ggcttggctg ccattggtaa tgccaaagtc
      781 gccacgcgta cttagcgtgg tcggggtcca ggctggctgc gtgccagttc cggccgccgt
      841 cggttgaaat ctccacgcgg gcaattgcgg actcgcctgc ccagctcgcc ccccgaattg
      901 tcacgccgtg ctgtgacggt tcgagagcgg cgccgtctgc aggctcagca attatggatt
      961 tcactcgcag cgacgtgagc gacatcgttt tcacatggcc ggattgctgc gccgcggatg
     1021 cgaccggtat gcggtatcca ggatccatgt agaagccgtg gaactcatgt gaaagaacac
     1081 gaatctcctg caaccacttg attgacgccg aaccaatcca gccgggaacg agagcacgaa
     1141 cgggaaatcc gtgcgaaggg gtgagtggcg agccgttcat tttagtcgcc aggagcgtgc
     1201 tgcgttccat cgctttctcg gtgggaatgc tgcgaataaa ttccgacgca ccctgaggaa
     1261 cgatgtcaag gccgcgaaac gcgacatgcc tggccgttgg ctttagatga gcgtggcgca
     1321 gcacgtcagc gaggatgggg ccctcaaacg ccgcgtttcc gacggcgccc cgtccccacg
     1381 gcacgccgcc aatgcgtgga ctgaagttga ctcggctgtt gccggcgcat tcgagcgtgt
     1441 tgatgaccct ggaagtgggc agttgcatga agtcgtgaaa attaatggtc tggggccgtt
     1501 ccacctcgcc cgtgatggag agcgtccaag tagagggatc gacggtcgcc ggcatcagaa
     1561 gattgtttcg gacgaagaaa gtgtcaacag gcgtaatcca tgagttcagg agggacatgg
     1621 gcatttcgag cgtgagatac ttccgcgaat aaacgatcat gcggtcgtca gccgattggg
     1681 tttgctgagg caaagcggac gccgcgaccg agcggagaaa ttgctcggcg gcagagggtc
     1741 tttggaatgc agctagcccg accaaaccaa ggctatttcg cagaatttct cgtcttttca
     1801 ggttcctgtc caaacggggg cgaacctcct gcggcagggt atggtaccac ggccgacctt
     1861 gtattctttg ccgggttggc cgaacacatt tattctgtaa tccatagctc acccggcaac
     1921 tttatatgga tattgggagt atacgtccaa tttggaacat ttagaaagcc attgctcgca
     1981 taactatcta tcttttcaat gggatagact tggcggtttt cagcctagtt ggtaccaaga
     2041 ttgccaccga taattccgca ttttccttac cgtcagatcg ctgacacaga acccaggcta
     2101 agctgtcggg ggtgacagtt gggttcggga gccgaaagat gaagagaatc gctttttatg
     2161 gggcattgtg tacgcttttg gtgtgtgggt tggcgggatt cgcggcagcg caaacgacgg
     2221 gcggaacgct gcgcggagcg gtaactgaca gccaaggatt agcgattgcc gatgcgtcgg
     2281 tcaccatcac caaccaggag acaaaggtaa cgattccgct ttcgaccact gccgcagggc
     2341 tctacaacta cccagaccta cctgtggcca cctactcaat cactgttgag aaggacggct
     2401 ttcagaagtt catccggaat ggcattcaga ttttcgcaaa ccaagtaacc gaggtgaatg
     2461 ttgtcctgcc gcttgggtcc gtttcatcaa ccatagaggt tacaggctca acgccccttg
     2521 tccagacagg aacctcgcag atttccaacg acttcaccgc cacgcaagtg acacagctcc
     2581 ccaacccgga cccgtcgggt tcgcagttgc aactcgcatt gctggccccg ggggcgactt
     2641 cgcagcaggc cggcgtcttg ggtgaaggcg gttcgatcgg cggcacgcgg ccgcgcatga
     2701 atagcttcac gattgacggc gtggacgaca accgaattga tctcactggt cacacacaat
     2761 acgtgattcc ggaatccgtt tcagaattca acctgcttac gaaccaattc gctgccgaat
     2821 acggccactc ggccggcggg caattcaaca ccatcacaaa atcgggcacc aacgactggc
     2881 atggtagcgc ttgggaatat aacaacaacc gtaactacaa tgcctacgat aacctggaaa
     2941 agcaaaacgt cgatgccaat ggcagggtcc ttcctaagcc gcggtacgat ttcaatcggg
     3001 ctggcacaga gattggcggc ccgatcatcc acgataaact gttcatctat ggcgctttcg
     3061 cgcggattat tcaaggactg gcgacccaag ccgtggttca gcatacagtt accgccgccg
     3121 gtcttacgac gctagaatct gtctcggacc cggcagtgca agacatcttg aagcagttcc
     3181 cgaccgcggg ttcgagtgat ggttccgaac tagtgacggt acccggcact tcgtgcgcag
     3241 gcggttgccc aatccctaca gggccgatct ctccgctggc gccgaacttc ttcaatcaat
     3301 gggatttcat tgtcaatgcc gatgcaactc tggggaagca ttcactctcc tttcacgtcc
     3361 tctatgaccg caaccggcag ccgaacgtca acccggacac gccacaggct cagtttacgg
     3421 gagcgatttc aagcgattcc agaaagtatt tgttcaagga cacctgggtg atttcaaatc
     3481 ggttcgtgaa cgaattccgc accggttatt cccggcttgt taacagcttc ggagttcccc
     3541 ctgcgttcag taactttccc aacgctgagg tggacacact gggcatcaac gttgggcctc
     3601 agggctgctc tccgcagagc accatcctca atacctacca gtttgcggac aacatgagct
     3661 atgtggtagg gaaacatacc ctcaagtggg gagttcaatg gttacatgcc attgcgccta
     3721 ctaactttct ccctcgcgca cgtggggaat gggattacac gaacctcaat caactcgtga
     3781 acgactttgc tccgaatgga ttgaacggtg cgctgcgcgg cgcaggtgac cccctgtttc
     3841 gcggcaatca gaacggggcc tccggcttta tacaggacga ctggagggtg acgccacgcc
     3901 tgacgctgaa cctcggtttg cgctacgagt ggaacggtat tcctctcgac gataatttgc
     3961 agaacctgaa tgccatttca acccttcccg gagtgttcga gtttcgcacg ccaaaggcag
     4021 actacaacaa catcggacct cgcgttggat tcgcctacga tccgacagga aacggaaagt
     4081 gggcaatccg tgcgggtttt gggatttcgt acgaccagac accgcagaat ttcgtgacgc
     4141 tcagcttgcc gccgcagctc caaacagagc aggctcccca gataacctgc gctctcgccg
     4201 gcgcgccggc ttggtgcgcc aacttcagcg cagcaacgta tggccagccc ggtggtaata
     4261 cgggccaggg cttcttgcag ggtggaggag atctccaggt taatgttccc tgcacgaccg
     4321 tatccgactg ccgaacatca actcaagctc ttatcgtcga tgttgttgaa ccaaaagtgc
     4381 ttacgtggag cttgggcgtt cagcacgaag tgggctggaa ctcgagtctg gaagtccgat
     4441 acgtggggac gcggagcttg gaactgccca tacaagctcg gctgaacact cagagcggct
     4501 tcgccgcagg attgggagcc cttccaacct acctgagttc atccgatgtc ccggccactg
     4561 tggctgcacc ttcggcgaca cttgccgatt gggacaattt cgaaaacaac gccggcggcg
     4621 cggcgacacc gaacggctgt gccgacccgt ctcctttcaa gttcggagtg cagggctttt
     4681 gtggctcact ggtaacggca ttcccaccct tggctaacgg gatataccac ggtgtttccg
     4741 cggacttcaa tcaccgcgtg ggtcacggac taactttgcg ggccaactac acctattcaa
     4801 agaacatgga cgatgccacg aacgaactgt tcagcagtcg agtcaacccg cgacggccgt
     4861 cggattggga ggatctgaga aatgactggg cgcgttctac actggatgtt cccagcaagt
     4921 ttactgtctc ctgggtgtac gacctcccca cttcgcattc ggacaatggc tttgtgaggg
     4981 gttttgcgaa cggctggcag tgggagggta gttggcaact ctataatgga acgcccgtta
     5041 ccattttgaa tggtggggat agcaacggaa acctcgattc agccggtgat gagcccgtgt
     5101 tgaatccgaa ggcgagcggg aacggtgtat cgggcatcga cttcgtctgc aacgcaggag
     5161 cgggaggcgc ggtcacaatc gtctccgacc cgacgacctg cggggcgggc gacgactcga
     5221 acatcgtggg ctacgtggca caagattcaa ccgcaaggta tgtttccgca gggcttggaa
     5281 cgaggagcac ggtcccgcgc aacagtttcc gatcgccggg cgtcaatatg tggaatatgg
     5341 gatttcagaa ggacaccagg atcaccgaac gggttcacct ggcgttcaat gtgtctgcgt
     5401 atgacgtctt caaccaccgg aactacgcgt tggcgcaacc ggacgtgctg gaagcggcaa
     5461 acgggtcgtt tatcaacacg gtcaacaacg cgctgagcac aacatacacc aacatcaacg
     5521 ccgccggtag cggcttcctg gacaaaacac aattctcagg ggggtcacgc aaaatccagc
     5581 tgggagttaa acttactttc taaactcgat ccaaaaacaa gccgattcaa aaggggcccc
     5641 ttttctgggg ccctttttgt ttgttccagc tgatcctggt cggcagcagc ccttggcacg
     5701 cagtgtttcg cgttggaaac ttgcggaaaa ccacacaggt cgcgcggaaa gcaaggtgca
     5761 acttcgggcg catccagtgc gtataatagc ttgagccgaa aggcgaccat gcaggcaaaa
     5821 acctcgatga cgttcgggga agccgtgcgg attgcgaccg ccagcctgtg gtcgcataag
     5881 ctgcgcagta tgttgacgtt actgggtgtg gtcatcggcg taatgtccgt gatcgcagtg
     5941 gtaagcctga ttaacggact caacgactat gtggcaggcg cgatatcgtc caacctgagc
     6001 gcggacgtct tcatgatcag ccgcgcccct tcgaccatca ccaatgttga ccaatgggag
     6061 tcggctcaaa agcgcaggaa attccatttc gacgactacg agtatctccg agacaattgc
     6121 cgaagctgtg tggccgttgc cgccaacctg aataagattg gccaggtgaa atacggcacc
     6181 gactatctgg acgacagcgc catcatcgga ttcacctacg agatgccgca agtctatagc
     6241 cgcgagctgg cggaagggcg cgacttcacg cgactggacg ttgcacgcag cgccgctgtc
     6301 tgcgatgtgg gatacgatat cgtgaacaag cttctgcccg gtgtggatcc catcggcaag
     6361 gtgatccacg tggatactgg cgagtgcgaa atcatcggcg tggccaaaaa gagaggctcc
     6421 acgatggggc agtcgcagga caattgggtg atcatgccgc tcaccaccta tcagggtatg
     6481 tacggaacta atgactcgct ccgcctgtgg gtaaaggccg ccggcaagaa cgcgatcgat
     6541 cctacgatgg atgaggtgaa gttgctctta cgagcgcgcc ggcatgtggc ctatcgcgac
     6601 gccgacgatt ttgcgatgga aacgaatgcg tcgtttcttt cgttgtggag cagcatcagc
     6661 agcacttttt ttggcgtgac aatcggaatc gcctcaattt cgttgatcgt cggcggcatc
     6721 gtgatcatga acatcatgct cgtatcggtg acggagcgga cgcgcgaaat cggcttgcgg
     6781 aagtcgctcg gagctcgtca gagcgatatt cgcatgcagt tcctgatcga atcggcggcg
     6841 ctggcggcca ttggcggagc ctgcggcgtg atcctgggta tcattgcggc aaagattgtg
     6901 agcatgacga cggcgctgcc gagctcggtc cagatt
//
LOCUS       DATNDT010000500         8030 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2773687, whole genome shotgun sequence.
ACCESSION   DATNDT010000500 DATNDT010000000
VERSION     DATNDT010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8030
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2773687"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..238
                     /locus_tag="VN788_16385"
     CDS             <1..238
                     /locus_tag="VN788_16385"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HXU22162.1"
                     /translation="PGMGHCSGGPGPNSFDAFGPLAAWVEHGRAPSKIVASHVSKGTA
                     TFTRPLCPYPMTAHYMGHGDTNEAGNFVCRLPGH"
     gene            complement(507..583)
                     /locus_tag="VN788_16390"
     tRNA            complement(507..583)
                     /locus_tag="VN788_16390"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(547..549),aa:Ile,seq:cat)
     gene            817..1179
                     /locus_tag="VN788_16395"
     CDS             817..1179
                     /locus_tag="VN788_16395"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU22163.1"
                     /translation="MVERRRCKRFPFTIPVKVYGRTPRNHPFRDVTATMAVSLYGGLL
                     EMKPRVKLGQKILIVNSFTEEERECRVVYVDSNQRGRRKVAVEFAKAEGDFWHVYSPP
                     VALKPAAPPAIPAGQPAT"
     gene            1527..2888
                     /locus_tag="VN788_16400"
     CDS             1527..2888
                     /locus_tag="VN788_16400"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HXU22164.1"
                     /translation="MHHVVPLRNSPIVDERRRHSRHKPTSIVYVALGPGNGGILVNLS
                     AGGMSLQAVAKLNADTELDLNFRLQGTEQAIEAVGCVAWLDSTHKEAGLSFKKLPAST
                     EQQIAKWIQGQEQAVRNAPTEEKPHTVSAATGEPLRAGEPPRLPSQASIPAVFPSEKP
                     ESARPSFIPGILSDPPSELRQRDDFESAFDTMPPPPSAPPAMHFRTRPEERFESPTKG
                     PIELSAKHYELSIEPQALLPVEPEILPRDCLLPQSISSPPAIVAADLLAPAVPDPSAT
                     DLHRRRKFAIALAAGVMGILALIVMATSITRRSLHPVPGVSDAPTAPRALPPAAKPPE
                     EVSQTKPKAPAERPIRTTASSTASVDVDATAPVPVVRVAVAPAPHDNGFIERLRELLG
                     LDVATTIDPAAAALPVWTVRHSGFYYCAHSPAFRTLEPGAIMTQGQALQSGYQPKLGS
                     YCE"
     gene            3014..5098
                     /locus_tag="VN788_16405"
     CDS             3014..5098
                     /locus_tag="VN788_16405"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU22165.1"
                     /translation="MHRSFIDRLLLPAIFGLTTIIAALVLWQLLLTNRRVEIQANTKE
                     QALFVKAKTESELSARVLSLERLAGRWQEPDHPADRDMESDASLVMSGYPAYQAIEWI
                     DPAFHVLWAEPQRENQSDIGANLGVDPRQRAKLEDAIETGGVTSTAPTELRQGGRGFL
                     VFVPVYSQKKLRGFLVGVFRYQDLLSSILQDVAPDYWVSVSDGGEEIYSRRLAKTFRE
                     GPYVQEANIQVEGITWRVKAWPKSGVVAYARSPLPTLVFGGGILMAIVLGFAVYMTES
                     SLLHVRKLTASNLKLEKEIAGREQAEEALRQAQKMEAVGRLAGGVAHDFNNVLMVIRG
                     QAALSLNAAGLSAGLRQELTEIVKAADRASLLTRKLLAFGRKQVLQARVLDLNTLITQ
                     LAEMLPPVLGEDIRLKIDLHPDLGHVKADSAQLEQVIMNLVFNARDAMPDGGALTIET
                     ANCDLDEAWVSTHPGSRSGPHVVLSVRDTGHGMDEATQAHLFEPFFTTKDKSRGSGLG
                     LATVYGTVNQSGGCVTVSSKPGSGTTFQIYLPRVDEPVDVFVDAPTPLVRFVGHEERI
                     LVVEDDDAVRRMTREFLQLRGYTVLEARSATEAIQFVQSHKETIDLVLTDVVMPGMKG
                     RELVDRLGKLRPGLKVLYMSAYTEDDAINIGILSPGTAFIEKPFGPDELAGKVRDVLM
                     GNIHVSAAGNQI"
     gene            5219..5295
                     /locus_tag="VN788_16410"
     tRNA            5219..5295
                     /locus_tag="VN788_16410"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5253..5255,aa:Met,seq:cat)
     gene            complement(5747..5941)
                     /locus_tag="VN788_16415"
     CDS             complement(5747..5941)
                     /locus_tag="VN788_16415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22166.1"
                     /translation="MTKKKPQPTIRRLLITHVILRLYLLPRDCSPLQSAIISFKDDSS
                     GVETLWSLAGAESEELTRST"
     gene            6192..6389
                     /locus_tag="VN788_16420"
     CDS             6192..6389
                     /locus_tag="VN788_16420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22167.1"
                     /translation="MTGAAAGGLPGELTSVCKHSEGLLSHPYCIDTVPVRVPVAAGVK
                     VTLSEQLVPAAREAPQVLVWA"
     gene            complement(6816..>8030)
                     /locus_tag="VN788_16425"
     CDS             complement(6816..>8030)
                     /locus_tag="VN788_16425"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU22168.1"
                     /translation="GATVINGYMVREVAMSRSMIGLGFTLLNLFVGLAAVLVAISVVK
                     FGLRATFVSGSAMICAGSLFMGLYATKPWHYLVAFGVVVGIGISFATLVPAATAVARW
                     FRRYRGRAMGIALSASGFAGLAVSPFLDKMLRTAGGNWRTGWDIVAGAVVIAGIIAFL
                     FVRESPESMGQLVDGIPEEQQAQFSRTDALATKHAWTAAQAYRTPAYWLIAIAGIMAT
                     FPFFLFVAHWILRLRGAGISSADAALAMGLFTLGTLGGRWLGGLLMDFMNARLAFVLG
                     LSVYFVGSYLAIIVRSDTLMIAYIAAILYGGAYGWAFTCTGTVTAHYFGPAAFPKLYG
                     TMTLLISAGACPAGYVGGRIFDAYGSYRQAFELNCVMAAIGIIAIAFATMPRPRNAAV
                     ASTDTLAQTQTA"
BASE COUNT         1760 a         2264 c         2328 g         1678 t
ORIGIN      
        1 tcctggaatg ggccattgca gcggcggtcc gggcccgaac agttttgacg cgttcggccc
       61 tctggcggct tgggtcgagc acggccgcgc tccgagcaag attgtcgctt cgcacgtcag
      121 caaaggaacc gcgacgttta cccggccact ttgtccctat ccgatgacgg ctcactatat
      181 gggacacggc gacacgaacg aggcggggaa ctttgtgtgc aggctgcccg gccattagct
      241 cgctggccct tggtgtacca gcgattcttt tcagtgatgt gtggattttg ggtagcctat
      301 cgctctgact aagtacgtgc ccatggattc aagcttatgc cgggtctcta ccatcagaga
      361 acatggcatt caggttcgtc ttcgctgggt atttcgtagc gcgacaccgg ctgccgtggg
      421 cgcgcaaggg tgacactgcc gcgcctccct cactccggga aactcgattc tatctgacgt
      481 cttaagtcct tgattcgatg agttgttggt gggcccgcta ggattcgaac ctaggaccaa
      541 cggattatga gtccgctgct ctaaccagct gagctacggg ccccgcgctc aggcaacata
      601 ttacatcgaa gagatttatt gcgaatcttt gttcttcgat tggcatttgg tgaaaagcgg
      661 gctagcagct cggcgtacat ctttcgccgc ctaatgctca actgcggtcc agggttatac
      721 agttaatcaa cggagtacca ttgcgttggc gcgttgccag gcctaacttc aatttgcgcg
      781 aaatgctcaa tcccagcgct ttcgaaggag gttgccatgg ttgaacgccg ccgttgcaag
      841 cgcttcccgt ttaccatccc cgtgaaagta tacggacgga cgccccgaaa ccatcccttc
      901 cgcgatgtaa ccgcgacgat ggcagtgagc ctgtacggtg ggctgctcga gatgaagccg
      961 cgcgtaaagc tgggccaaaa gattcttatc gtgaatagtt ttaccgaaga agagcgcgag
     1021 tgccgggtag tttacgtcga ttccaaccag cgcggccgaa ggaaggtcgc ggttgaattc
     1081 gcgaaagcgg aaggcgattt ctggcacgtg tacagtcctc ctgtggcact caagccggcg
     1141 gcgccgccgg cgattccggc cggccaaccc gcgacctgag cttgtcttca aagctcttat
     1201 ccaggaaaca cagccaactc cacgctccgc ccacagggtc cgatgtccgt aaaaatcatg
     1261 cgagcgctaa cacgggcacc ggaagcccga gtttaggcca tcaatgtttc ctgccacagg
     1321 aatctgggga agccagaatg caaaaaacag ggggttgcaa gcgtagattt cggctagaat
     1381 aagaccgcta gcaggctgcg gagcttgccg cgacgatcgg tcaattagtt tcttcaaatg
     1441 gacggtcgcc cggctgaaga aaaatggcgc cccggtcttt tatcgacgcc gttgattctc
     1501 aataaccggc gtgtcggcag gcgaaagtgc accacgtcgt acctctgcga aacagcccga
     1561 tcgtcgacga acgtcgccgt cactctcggc ataagccgac ctcaatcgta tacgttgcgc
     1621 tcgggccggg taacgggggc attctcgtca accttagtgc aggggggatg tccctgcaag
     1681 cggtcgcgaa actgaatgcc gacacggagc tggacctaaa tttccgatta caaggcaccg
     1741 agcaggcgat agaggcggtg gggtgcgttg cgtggttgga ttcaacgcac aaggaagccg
     1801 gactctcttt caagaagctc ccagccagca cggaacagca gatcgccaag tggattcagg
     1861 gtcaagagca ggcggtccgg aatgccccga cagaggaaaa gcctcacacc gtatctgcgg
     1921 caacaggtga accgctccgg gcaggtgaac cgccccgcct tccgagccaa gcgtccattc
     1981 ctgcggtttt cccttcggag aaacctgaga gtgcgcggcc aagttttatt ccaggtattc
     2041 tgagcgaccc gccttcggag cttcgccagc gggatgattt tgagagcgcg tttgacacga
     2101 tgccgccgcc tccgtcggcg cctccggcaa tgcatttcag aacgcgtcct gaagaacgat
     2161 tcgaatcgcc aaccaaaggg cctattgaat tgtcggcgaa gcactacgag ctgagcatcg
     2221 agcctcaagc tctactaccg gtcgagcccg aaatccttcc cagagactgt ctgttgccac
     2281 agtcgatttc ttcaccaccg gcgattgttg cagcggatct cctggcgccc gcggtgcccg
     2341 atccatctgc aacggactta caccggcgac ggaagttcgc gattgcgctg gcggcgggcg
     2401 taatggggat ccttgcactg atcgtaatgg caaccagtat tactaggcgt tctctgcatc
     2461 ctgtgcctgg cgtctcggac gcccccacgg ctccgcgagc attgccgccc gccgcaaaac
     2521 cgcctgaaga agtgagtcaa acgaaaccga aggcacccgc tgagcgccct attagaacga
     2581 ccgcgtcctc aactgcttcg gtcgatgtcg acgcaaccgc cccggtaccg gtggtgcgtg
     2641 tggccgtcgc gcccgcgcca cacgacaacg gattcataga gcgcctgagg gaacttctcg
     2701 gcctggatgt cgccactacg attgatccag ccgcagccgc gttgccggta tggacggttc
     2761 gacatagcgg attttattac tgtgcccaca gtccggcttt taggacgcta gagccagggg
     2821 ccatcatgac acaaggtcaa gcgcttcaaa gtggatatca accgaaactt ggcagctact
     2881 gcgaataagc attcctcgag ttgcaacctg caactgtttc tggggcatcc aaggcgctgg
     2941 ctatttggta gcggagtcga gaacggttgc gaggcggcgc cgtttttacg ttatgcactt
     3001 ttcgagggta cggatgcaca gaagctttat cgaccggttg ttgctacccg ccattttcgg
     3061 cttgaccact atcatcgctg ctttagtact ttggcagctt ttgctcacca accgtcgtgt
     3121 cgagattcag gccaacacga aagagcaggc tttatttgta aaagccaaga ctgaatcgga
     3181 gttgagcgcg cgcgttctgt cgctagaacg tttggcgggg cgctggcagg aaccagacca
     3241 ccctgctgat cgagacatgg agtcggatgc tagcctggtg atgagtggct acccggccta
     3301 tcaggcaatt gaatggatag atccagcctt ccacgtgctc tgggcagaac ctcagagaga
     3361 gaatcaaagc gacattggtg caaaccttgg cgttgacccg cgacagcggg cgaagcttga
     3421 ggatgcgatt gaaactggcg gcgtgacctc gaccgctcca acggagctgc gacagggagg
     3481 tcggggcttc ttggtcttcg tgcccgtgta ctcccagaag aagttgaggg gtttcctggt
     3541 tggcgtgttt cgctaccaag atctgctctc ttccatcctt caggatgttg cgcccgatta
     3601 ttgggtctcc gtttccgatg gtggggagga aatctacagc cgccgactgg ccaagacctt
     3661 tcgagaaggg ccctatgttc aggaagccaa tatacaagtc gagggaataa cgtggcgagt
     3721 gaaggcttgg cccaagagcg gggtggttgc atacgcgcga tcacctttgc cgacgctggt
     3781 tttcggcgga ggcattctga tggctatagt cctaggattc gctgtttaca tgacggaatc
     3841 atcactgctc catgtgagga aactgacggc ctcgaatctt aaactcgaga aggaaattgc
     3901 cgggcgcgag caagccgagg aagcgctgcg tcaagcgcaa aagatggagg cggtcggccg
     3961 gttggccggt ggcgtcgctc acgacttcaa taacgtgctg atggtcatcc gcggccaagc
     4021 tgcgctctcg ttgaatgctg ctggcctcag tgccgggttg cgccaggagt tgacggaaat
     4081 tgtgaaggcc gccgaccggg cctctctatt gacaaggaag ctcttagcct tcggccggaa
     4141 gcaggtactc caagcccgcg tattggatct caatacccta attacccagc tggcggaaat
     4201 gttgccaccc gtgctcgggg aggatattcg gttgaagatc gatctccatc ccgacctggg
     4261 ccacgtcaag gccgattcgg cgcaattgga gcaggtcatc atgaatctcg tctttaatgc
     4321 gcgagatgcc atgccggatg gcggcgcgtt gacgatcgag accgcaaact gcgacctcga
     4381 tgaagcgtgg gtcagcactc acccgggaag tcgaagcggt ccccacgtag tgcttagtgt
     4441 gcgcgatacc gggcacggca tggacgaagc cactcaggca cacctctttg aacccttctt
     4501 cacaacgaag gacaaaagca gaggttctgg actcggtttg gcaactgttt atggaacggt
     4561 aaaccaaagc ggcggttgtg ttacggtctc cagcaagccc ggatcgggga caacatttca
     4621 aatctacctt ccgcgagttg acgaaccggt tgacgtattt gtcgacgcgc cgactccgct
     4681 ggttcgcttt gttggacacg aagaaagaat cctcgtcgtt gaagacgacg atgcggtacg
     4741 tcgcatgacg cgcgagtttc tgcagttacg gggatacacg gttctcgagg cgaggagcgc
     4801 caccgaggcc attcagttcg tgcaatctca caaggaaacg attgatttgg ttcttactga
     4861 tgtagtcatg cccggaatga aggggcgcga attggttgat cggttaggca aactacgccc
     4921 ggggttgaag gtgctctaca tgtctgccta taccgaagat gacgccatca atatcgggat
     4981 tctgagccca gggacggcat tcattgagaa gccctttggt ccggatgaac tggctggcaa
     5041 ggtgcgcgac gtcctgatgg gtaacatcca tgtttctgcc gctggtaatc aaatctgaag
     5101 aatcttgatt cgcactcctc cagcgcaaat tgcgctgtcc tcggaacgaa gccggtcaga
     5161 catcttagtt atttccaggt gcgtttgacc gtatctcaag catccgctat gatgagttcg
     5221 cggggtggag cagcctggta gctcgtcggg ctcataaccc ggaggtcgtc ggttcaaatc
     5281 cgacccccgc aaccaactgt cttatgcagc tagaagggtt tccgatttgg agagaatcgg
     5341 gcccccttct catttcacgg cgttttcgat ttgttcaccg gcaccggtta tgccgcctcc
     5401 acgttggttt tccattccga gtgtacacat caagcgtcaa ggatccggcc gaggcatgtc
     5461 cttgtcgttt ctgaatcgtt ttttggtggc acacgaagct cggagagaag ggaagcgttg
     5521 aaatgccgaa atgcatgcag ttcaccgtca cgaaactgaa gctcttgcag caggggcttg
     5581 aattttcgtt ggcagaacaa actgacagat agtcgcgctc ccagactttt gagaatgcct
     5641 cgaacgttgc gctccacgga gtcggcggca aatcgttctc ggtcccgtat ggaaaaatgt
     5701 acagcacaga gatgcggaaa cgggaggcgt tgcggatttt gcagcgctac gtcgaccgcg
     5761 tcaattcttc actttccgcg ccggccaagg accacagggt ttcgacgccg cttgaatcgt
     5821 ccttgaacga aatgattgca gactgaagcg gagaacagtc gcgaggaagc aagtagaggc
     5881 gtaaaatcac gtgagtgatg agtaagcgcc gaatagtggg ctgcggtttt ttcttggtca
     5941 ctgcgatctt acttcttgaa gtttggcatg cccatcacaa gcgagcggca ccagaaaata
     6001 ctatgggtgg agttgaagta tatacgtgca tcgacgcttc agggacaaag tggtggcccg
     6061 cgcacgtcac ctatgacgca cagggcaatg tgctgagtac tgaatgccgc atccaagacg
     6121 caacccctga gccttgaagt tcactcgagg tatgcccgta ccgctgagac aatgtttgtg
     6181 ttggcgcttg aatgacaggt gctgccgccg gaggtctacc gggggaactg acttccgtct
     6241 gcaagcactc cgaagggctg ctatcgcacc cgtactgcat cgatacggtg ccggtgcggg
     6301 tgccagtggc agcgggtgtg aaggtgacgc tcagcgagca gttggtgccc gccgccagag
     6361 aagcgccaca ggtgttggtc tgggcataat cgccgctagc cgagatgccg ctgatggtca
     6421 gagtggcagt gcccgagttg gtgagagtca ccatctgtgc cgcgctggtg gtacccacgg
     6481 tctggctgct gaaggtgaga cgtgtggtgg acagcgttaa cttccgaggt tggttcccgc
     6541 tacttggggc acctactgcg aaaatcattg tcgcgagagc gaccgcgcgc gcagaaaatc
     6601 tcgccaggga tgttatatcc ggaatgccgt ggtttggtcc tcggcaacgg ccgaacaact
     6661 ctcgtatccg cgcacggtgc tgggctggcg gcacgtagct cgaatttctt cttaccttgg
     6721 aacctaaata tggcggacac gctaacacac acggcattgc acgtaaatct ttatgaatgc
     6781 ctgatcgcgc ctcagtcggg tggggcggcg agtggctagg cggtttgcgt ttgtgcgagc
     6841 gtgtcagtcg atgccactgc cgcattccga ggacgcggca tggttgcgaa tgcaatcgcg
     6901 atgataccga tcgcggccat cacgcaattc aattcaaacg cctgcctgta gctcccgtaa
     6961 gcgtcaaaaa ttcttccgcc cacataaccg gccgggcagg cgccagccga aatcagcagt
     7021 gtcatcgtgc cgtacagttt aggaaaagcg gcgggaccga agtagtgcgc cgtcaccgtc
     7081 ccggtgcaag taaacgccca gccgtaggct ccgccataca aaatcgccgc gatgtaagcg
     7141 atcatcagcg tatctgaccg cacgatgatc gcgagatagg agccgacgaa atacacgctg
     7201 agtccgagca cgaaagccag ccgggcattc atgaagtcca tcagcaatcc accaagccag
     7261 cgtcctccga gcgtgccgag cgtaaacaac cccatcgcca gagctgcgtc ggcggaactg
     7321 atgcccgcgc cgcgcaaacg cagaatccaa tgcgcgacga acagaaaaaa tgggaatgtc
     7381 gccatgattc cagcgatcgc aatcagccag tacgcgggcg ttcgataggc ctgcgccgcg
     7441 gtccatgcgt gcttggtcgc cagcgcatcc gtgcgcgaaa actgcgcctg ctgctcttcc
     7501 ggaatcccgt ccacgagttg ccccattgat tccgggcttt ccctgacgaa aaggaaagcg
     7561 ataattccgg caatcaccac cgcccctgcg acgatgtccc aacctgtacg ccaattgcct
     7621 cctgcggttc gcagcatttt gtctaggaag ggcgacaccg cgagcccagc gaagcccgaa
     7681 gcggaaagcg caatccccat cgcccttccg cgataacgcc tgaaccatcg agccacagcc
     7741 gtcgcggccg gaaccagcgt cgcgaagctg attccgattc caaccaccac cccgaacgcc
     7801 accaaatagt gccacggttt ggtcgcgtag agccccatga acaaagatcc ggcgcagatc
     7861 atcgccgagc cggaaacgaa cgtagcccgc agcccaaatt tcaccacgga tatagccaca
     7921 agcaccgcgg ccagccccac gaacaaattg agcagcgtaa acccgaggcc gatcatgctc
     7981 ctgctcatcg caacctcgcg gaccatgtag ccattgatca ccgtcgcgcc
//
LOCUS       DATNDT010000501         3586 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3478005, whole genome shotgun sequence.
ACCESSION   DATNDT010000501 DATNDT010000000
VERSION     DATNDT010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3586
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3478005"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VN788_16430"
     CDS             <1..619
                     /locus_tag="VN788_16430"
                     /inference="COORDINATES: protein motif:HMM:NF019157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HXU22169.1"
                     /translation="YSDGKQIFPGFEPGSELAWGMMAGGPQPISIGTGFFAYMVFANP
                     NWDFRTFDVDRDTRLADSRLGSTVNAIDPNLKPFESHGGKIIMYHGWADQAIMPKNSL
                     NYYESVVHALGGQQKTQDFLRLFFVPGMGHCQGGPSPNTFDSLTALEQWREHGAAPEK
                     IVASHLTKGVVDETRPLCPHPQVAIYKGSGSTSDAANFTCGNPNW"
     gene            complement(759..1565)
                     /locus_tag="VN788_16435"
     CDS             complement(759..1565)
                     /locus_tag="VN788_16435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22170.1"
                     /translation="MTEVPTPAETAIEKRRSTRVVRAFPITVIGVDALQEPFMENTNT
                     VMVSCHGCKYQTAHYVPRGSALEVEIAATRRGAPPRRVAAKVIWVQRPRNAKDLLHVG
                     LDFEVAGNVWDIPSPPRDWFPVPGEGQLSKSEPDETLVPCSGMQPPETEQSIESEIAT
                     AEWDEREILSVSKTVDVIEPKTTTKIEAEPIGPLDITSSGPLVNEEIRELVERTVKAA
                     VDRLAKSVVEEARNERIAIAAQLDAKVQQAVEAAITRLPAIQLKKSKRRN"
     gene            complement(1663..>3586)
                     /locus_tag="VN788_16440"
     CDS             complement(1663..>3586)
                     /locus_tag="VN788_16440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22171.1"
                     /translation="VKQTSENEPKVLQQRSEALARTLNIKVDQGCFYKPAEQQAPCLM
                     QNTDQLVLDDGHTQSMVAALTTGPSSDLIGAVSATPLAAGGTFSPYVGAVVDMARLMG
                     TLHTAEYQYIPALALPRQNQLNLRLNSPPSFRNPKSVLVIGLPAVEAPQLPPLRAVNP
                     DEVLCLQKTPLVLPVQGAPLVFSTTIAHDFVLRVPTMNGQTIDLPARADAARGGFVID
                     IHALPPGISTSQVKGTLRGFWGFDMYEGPSFNLRRAQAGHWMVSAEDRSALIADRDDT
                     LHLKSECAVCVDRVTVENAQGKNLTATWKIAKPDELEVQVPLKGIAAGPIKLKVKQFG
                     VADPDELQLVAYSEAAHLDHFAINTGDRRGVLTGTRLDEVQKLKLGEMEFVPGKLSRA
                     DQKDELPLLASNSDMPLAAPPHQKLVAYVSLKDGRVLNLQTTVEPARPKVDLIRKSIQ
                     TGPSGSAIRFASQDQLPQDGRISFFLKTEIPDKFPRTEKIEVANEDESFSTLLSIGDG
                     NIIMQDSQTVLGVVDPLKSFGPSAFGALRFRAVGEDGAKGDWQPLADLVRVPALKEIR
                     CPDSPDKPCTLSGTNLFLIDSVASDSQFTHTTPVPLGFADSTLTVPRPNGTLLYIKLR
                     DDPSIADAVVLPVLPE"
BASE COUNT          747 a         1019 c         1006 g          814 t
ORIGIN      
        1 gtattccgac gggaaacaga tatttcctgg cttcgagccg ggaagcgaat tggcgtgggg
       61 catgatggct ggcggtcccc agccgatcag cattggcacc ggttttttcg cgtacatggt
      121 gttcgcgaac cctaactggg attttcggac cttcgatgtg gatcgcgata cgcgactcgc
      181 ggattcgcgt ctcggttcaa ccgtgaatgc catcgatccg aacttgaaac ctttcgagtc
      241 gcatggcgga aaaatcatca tgtaccacgg ttgggccgat caagcgatta tgccgaagaa
      301 cagcctcaat tattacgaaa gtgtggtgca tgccctgggc gggcagcaaa agacacagga
      361 ttttctacgg ttgttttttg tgccgggaat gggacattgc cagggcggac cttctccgaa
      421 cacgttcgat tcgcttaccg ctctcgagca gtggcgcgag catggtgcgg ctccggaaaa
      481 aatcgttgcg tcgcatttaa cgaagggcgt tgtcgatgag acgcggccgc tctgcccgca
      541 tcctcaggtg gctatctaca agggcagcgg cagcaccagc gatgctgcaa acttcacgtg
      601 cggcaatccg aactggtaaa taaatctaag ggaaatcgga aggtcccgcg agtatctctg
      661 agactcatgg aaaggttctg cccgaattgc gcgcgaacgc acgactccgg ttcgtgcaca
      721 ccgaatcgcg tgcgtccgaa tgatcaattg cctcgtttct aatttcgtct ttttgatttt
      781 ttgagctgga tagcaggcaa tcttgttatg gcggcctcca cggcttgctg gaccttcgcg
      841 tccaactgcg cagcgatagc aatacgctcg ttgcgggcct cctccaccac cgacttggca
      901 agccggtcga cagccgcttt aacggtcctt tcgactagct cgcgaatctc ctcatttacc
      961 agaggaccgc tggaggtaat gtccaggggc cctatcggtt cagcttcgat ttttgtcgtg
     1021 gtcttcggtt caatcacatc gactgttttg gacacagaaa ggatttctct ttcatcccac
     1081 tcagctgttg cgatttcact ctcgattgac tgctccgttt cagggggctg catacccgaa
     1141 catggcacca atgtttcgtc gggttcggac tttgaaagct gtccctcgcc gggaactgga
     1201 aaccaatccc gaggcggcga cggaatgtcc cagacattac ctgctacctc aaagtccagc
     1261 ccaacgtgca gcagatcctt cgcattgcgc ggcctttgaa cccagatcac tttcgccgca
     1321 actctccgtg gcggagcacc tcggcgggtc gcggcaattt ctacctcaag ggccgagcct
     1381 cgcgggacgt agtgtgcggt ttggtatttg cagccgtggc agctcaccat cacggtgttc
     1441 gtgttttcca taaacggctc ttgcagcgcg tctacgccaa ttactgtgat cggaaaagct
     1501 cgcaccacac gcgtgctgcg gcgcttctcg attgctgtct cggccggtgt cgggacttcg
     1561 gtcaagtccg cctcagaggg gatctaggac ggggaaaatg atatcaccgg ctcgggcggc
     1621 tactcacgtt ctcgtcaaag cgatagcgcc gaaaagttac tgttactccg gtagcaccgg
     1681 aaggactacc gcgtccgcga tggatggatc gtcgcgcagc ttaatataca acaaagtgcc
     1741 gtttggccgg ggaacggtga gtgtactgtc ggcgaaaccg agcggcaccg gtgtcgtgtg
     1801 ggtgaattgc gaatcggagg caacagagtc aatcaggaag agattcgtcc cgctgagtgt
     1861 gcaaggttta tccggactgt cagggcagcg aatttccttg agcgcgggga ctcgcaccag
     1921 atcggcaaga ggttgccaat ctcccttggc tccatcctcg ccgaccgcac ggaagcgcag
     1981 ggcgccaaat gcagacggcc cgaaactttt cagcgggtcc acgacgccaa gaactgtctg
     2041 cgaatcctgc atgatgatat tgccgtcgcc gatgctgagt aacgtgctga acgactcatc
     2101 ctcattggcc acttcgatct tttccgtccg cggaaactta tcggggattt cggttttcaa
     2161 gaaaaaagat attcggccat cttgagggag ttgatcctgg ctcgcgaagc gaatggctga
     2221 gccgcttggg cccgtctgaa tgctcttcct aatcaaatct actttgggtc gcgccggctc
     2281 aacggtcgtt tgcagattga ggacgcggcc atccttgaga gaaacataag cgactagttt
     2341 ctgatggggc ggcgcagcca gcggcatgtc ggaattcgaa gccaagagcg gcaactcgtc
     2401 tttctggtcc gcgcgcgaca gtttccctgg cacaaactcc atttcaccca gcttgagctt
     2461 ctgcacctcg tccagccggg tgccggtaag gacgccccga cggtcgccgg tattgatcgc
     2521 gaagtgatca agatgagcgg cctcggaata cgcaactaac tggagttcgt cgggatcagc
     2581 cacgccgaac tgtttcactt tcaatttgat tggccccgcc gcgatgccct ttagcggtac
     2641 ctgcacttcc agttcgtccg gcttcgcaat cttccacgtc gcagtaagat ttttcccctg
     2701 cgcattttca accgtcactc gatcaacgca cacagcgcat tccgatttga ggtggagcgt
     2761 atcgtctcgg tctgcgatga gagcgctcct gtcttccgcg gacaccatcc agtgtcctgc
     2821 ttgcgcccta cgcagattga aactcgggcc ctcgtacatg tcgaagcccc agaagccacg
     2881 aagcgtcccc ttcacctgcg atgttgagat acccggcggc aaggcatgta tgtcgatcac
     2941 aaagccgccg cgcgcggcat cagcccttgc cggcaggtca atagtctgcc cgttcattgt
     3001 cggcactcgc agcacaaagt catgcgcaat cgtggttgag aacaccaatg gcgcgccctg
     3061 gacgggaaga acgagcggcg ttttctgcag acagagaact tcatccggat tgacggcacg
     3121 cagcggagga agctgcggcg cttccaccgc cggcaaccca atcaccagga cggatttcgg
     3181 attccgaaac gaaggcgggc tgtttaatcg aagattcagc tggttttgcc tgggcagcgc
     3241 aagcgccgga atatactgat attccgcggt atgcaaagta cccattagtc tggccatgtc
     3301 caccaccgcc cctacatagg ggctgaaagt tcctccggca gcaagagggg tcgcactcac
     3361 agcgccgatc aaatccgatg atggacctgt ggtcagggcg gccaccattg actgagtgtg
     3421 cccgtcatcc agtacaagct gatcagtatt ttgcatcagg caaggagcct gctgctccgc
     3481 gggcttgtag aagcatcctt gatcgacttt gatgttcagc gtgcgggcca aagcttccga
     3541 ccgctgttgg agcaccttgg gctcgttttc agaggtctgc ttaact
//
LOCUS       DATNDT010000502         2428 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2161374, whole genome shotgun sequence.
ACCESSION   DATNDT010000502 DATNDT010000000
VERSION     DATNDT010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 2428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2428
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2161374"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2428)
                     /locus_tag="VN788_16445"
     CDS             complement(<1..>2428)
                     /locus_tag="VN788_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013579092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HXU22172.1"
                     /translation="DLMELKSLEDWVLEKQFKSVPNVVDVSSFGGPTREYQVRVDPDK
                     LVSYGLSIGQVEQQLAANNVNAGGSFIEVGLQQINVRALGLIRNVKDIEQTVIATHHG
                     TPIRVRDIATVEQGAKIRLGRIGKAIHRADGKIVDDDDVVEGIVLLRKGAESDATLDA
                     IHAKVRELNDHILPSGVKIVPFLDRSNLVHYTTHTVLHNLGEGIVLVAVILFLFLGNV
                     RGAFIVALTIPFSLLFAAACLKAAHIPANLLSLGALDFGMVVEGAVVMVENIVRYLSR
                     RNGHAENSDLIRAAAHEVQRPVFYSIGIIITAYLPIFTLQQVEGRLFKPMAWTVTFAL
                     SGALIFSMLIAPAISSFAFGEHIREWRNPAMTAMTRAYRRAVRWTIDFRWVTAGVALG
                     ALALSAYLAWSGIIGSEFLPHLDEGAIWARGTLAPSTGPSEGERVMNQARLIFASFPE
                     VTQVVSQVGRPDDGTDTTGFFNTEYFVDLKPKDQWRSVFRQNKEELIGAMDRELEKIP
                     GTLWNFSQPIADNMEEAVSGVKGQLAVKIYGDDLKTLEAKGDEIVNVMRTVPGIADLG
                     LFRVIGQPNLEFVVDRGEAARYGINVSDVQDAVETAVGGKAVSQVLEGEQRYDLVVRY
                     QEPYRNTKEAIENIRLPAPSGERVSLAQLCKVTVADGASEIYREGNSRYVALKYSVVG
                     RDLGSTVEEAIRKVTRQVKLPPGYSIDWAGEYASQKRSQKRLMIVLPLTILAICMILY
                     TMFSSLKWVLLILANVAMAPIGGLLALLITGTHFSVSSGVGFLALFGVSVQTGVIMLE
                     YINQLRVRGHA"
BASE COUNT          526 a          760 c          652 g          490 t
ORIGIN      
        1 gcatgtccgc ggacgcgtag ctggttaatg tattcaagca tgatgacgcc agtctgtacc
       61 gacacgccga acagcgcgag aaaaccgacg ccggaggaga cgctgaagtg agtaccggta
      121 atcagcaagg ccagcagtcc gccgatgggt gccatcgcga cgttggcgag aatcaacagc
      181 acccatttca gcgagctgaa cattgtataa agaatcatgc aaattgctag gatcgtgagc
      241 ggcaacacaa tcatgagccg cttttgagat ctcttctggc tagcgtactc accggcccaa
      301 tcgatgctgt agcccggcgg gagcttcact tgccgcgtca cctttcgaat cgcttcttcc
      361 acagtgctgc caagatcgcg gcctaccacg ctgtacttga gcgcgacgta ccgggaattg
      421 ccctcgcgat agatttccga cgctccgtcg gcaaccgtga ccttgcatag ctgcgcaagc
      481 gagacgcgct cgccggaggg ggccggcagc cggatgtttt caatcgcttc ctttgtattc
      541 cggtagggtt cctgatagcg cacgacgagg tcgtagcgct gctcgccttc gagcacctgg
      601 cttaccgctt tccctccaac ggccgtttcc acggcgtcct gaacgtcgga tacgttgatg
      661 ccataccgcg ccgcttcgcc gcgatccacg acgaactcca ggttcggctg gccgatcacc
      721 cgaaacaacc cgagatcggc aatgccgggg acggtccgca ttacgttcac gatctcatcg
      781 cctttcgcct ccagcgtctt cagatcgtcc ccgtagatct tcaccgcaag ctgccctttc
      841 acgccgctga ccgcttcctc catgttgtcc gcgatgggct gcgagaaatt ccacagcgtg
      901 ccgggaattt tctcaagctc ccgatccatg gcgccaatca actcttcttt gttttggcgg
      961 aatacggaac gccactggtc cttaggcttg agatccacaa aatattccgt gttaaaaaac
     1021 ccggtcgtgt ctgttccgtc gtcgggacgg ccgacttgcg acacaacctg cgttacttcc
     1081 ggaaacgacg cgaagatcag ccgcgcttgg ttcatcacgc gttcaccctc ggaaggtccg
     1141 gtactcggag caagcgtgcc tcgcgcccag atcgcgcctt catcaaggtg cggtaggaac
     1201 tccgaaccga tgattccgct ccacgcgaga taagccgata gcgcaagcgc gcccaacgcg
     1261 acgcccgccg tcacccaccg gaagtcgatc gtccatcgca cggcccggcg gtacgcgcgc
     1321 gtcatcgccg tcatcgccgg attacgccac tcgcgaatgt gctctccaaa agcgaagctc
     1381 gatattgccg gcgcaatcag catggaaaaa ataagcgcgc ccgacaacgc gaaagttacg
     1441 gtccaggcca tcggcttgaa taggcgccct tccacttgct gcaacgtgaa gatcggcaga
     1501 tacgctgtga taatgatgcc gatcgagtag aaaaccggcc gctgcacctc atgcgcggcc
     1561 gcgcggatta ggtcggagtt ttcggcgtgg ccattccggc ggctgaggta gcggacgatg
     1621 ttctccacca tgacgaccgc gccctccaca accatgccga aatcgagcgc tcccagtgag
     1681 agcagattcg ccggaatgtg cgcggccttg aggcacgccg cggcgaaaag caaagagaac
     1741 ggaatcgtga gagctacgat gaacgcgcca cgcacattgc ccagaaaaag aaaaaggatc
     1801 actgcaacga gaacgatgcc ctcgcccagg ttgtgcagga ctgtgtgggt ggtgtagtga
     1861 acaaggttgc tgcggtcgag aaaaggaacg atcttcacgc ccgaaggaag aatatggtcg
     1921 ttcaattccc gcacttttgc gtggatcgca tcgagagtcg cgtccgactc agctcccttc
     1981 cgcaaaagaa cgatgccttc aacgacatcg tcgtcgtcca caattttccc atcggcacga
     2041 tgaatcgctt tgccgattct ccccagcctg atcttcgcgc cttgctccac ggtcgcgatg
     2101 tctcgcactc gtatcggcgt gccgtgatgc gtggcgatga cggtctgctc aatatccttc
     2161 acgttccgga ttaaaccaag ggcgcgcacg ttgatctgct gcaggcccac ctcaatgaaa
     2221 ctgccgcccg cgttcacatt attagccgcg agctgctgtt caacctggcc aatgctcagc
     2281 ccgtaagaca ccaatttgtc cggatcgacg cgaacttgat attcgcgcgt ggggcctccg
     2341 aaactcgaaa catcgacaac gttcggcaca gacttgaact gcttctccaa cacccagtct
     2401 tcgagcgact tcaactccat caggtcgt
//
LOCUS       DATNDT010000503         3848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1011057, whole genome shotgun sequence.
ACCESSION   DATNDT010000503 DATNDT010000000
VERSION     DATNDT010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011057"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..1111)
                     /locus_tag="VN788_16450"
     CDS             complement(167..1111)
                     /locus_tag="VN788_16450"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HXU22173.1"
                     /translation="MADQKFKVFCGTANPTLCDCICSYLGVPRGKAEHGQFSDGETRY
                     QILENVRGADVFVVQPCANPVDLHLMQLLIMVDAFKRASAWRITPVIPYYPYSRQDRK
                     DRPRVAISAKLVADLLETAGSSRVLSLDLHAPQIQGYFNIPVDHLYAAPVLVEYFQKK
                     QLGPVTVVSPDAGGVERARLFAKRMDAPLAIVDKRRIDVNVSEVMHLIGDVKDRTALV
                     IDDIIDTAGTLVKTAEALLENGASKVYAACTHPVLSGPAVERIRESDIVEVVVTDSIP
                     LSQAARELPKIKTLSVAPLLARGIQSIHEETSISELFI"
     gene            complement(1121..1999)
                     /gene="ispE"
                     /locus_tag="VN788_16455"
     CDS             complement(1121..1999)
                     /gene="ispE"
                     /locus_tag="VN788_16455"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HXU22174.1"
                     /translation="MRLPAFAKINLCLHVIGRRPDGYHELRTIFQAISLCDTLELSRT
                     SNPGIFLETTDTTLTAGSDNLVYRAIDALGCEIGFRGGIRARLEKRIPVARGLGGGSS
                     DAAAALIGMLRLTKKRLPLDRLMAIGAGLGADVPFFLFGGRALAVNRGDEIYPLDDLP
                     KQTIVVVSPRNIGVSTKEAYQWMSAELTKSTKPPNIFEFCALCWSRQNRVFNDFEGPV
                     FGRYPRLGEIRDGLLKRGAANAALAGSGSAVFGVFRNPAQARRAARAFPEDSVFVTET
                     VSREKYGRAMGWSVTR"
     gene            complement(2034..>3848)
                     /locus_tag="VN788_16460"
     CDS             complement(2034..>3848)
                     /locus_tag="VN788_16460"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU22175.1"
                     /translation="AVIQERLAEIHAKSGHVREATEEAQAALKIDPDNVDAHRLLARI
                     YVRALGEASGGEVPQASITQAIEQFQAILKVEPDDTYSALWLARLYRFENHHSDAEKI
                     LRQVLQKDSENSAALEQLSQLLIDEGKSQEAIELLTQASTDSASPDIYDLLGSAYSQA
                     KDYAKAEAAYRKAVALDPDDAGHRHGLAEALMEQDKYADALEQYKKLVELEPGTSENH
                     LRLAQLYRRLGQFDKAQSSLERAKQLAPGSLEILFNQALLDEDQGHYDDAVKLLTDAI
                     AGIKSQSGGGSANALAILYEQLGRAYRGARNYPAALEAYADMGKLGGDSRKRAETLTI
                     ETYRENHNIDEAIAAAKKALDAEPKDPEMTVTLAMLYGEKTETTAATQLLQGLLRGND
                     GDQQIYVDIAQVQERGRKYMDAEQSAQKAEQMAHGDSGKETAWFLLGSIYERQKRFDQ
                     AEQQFRKVLQVNPDNAPVLNYYGYMLADRGIRLDEAGSLIERALKQEPNNGAYLDSLG
                     WAYYKQNKLPEAEENLRKAIDRQGNDPTILSHLGDVYLKLGQKERAAETFELALAEWR
                     KAVPADYEADKVTELEAQLRSLKKHLAQKSSPDSAKPQ"
BASE COUNT          742 a         1255 c          974 g          877 t
ORIGIN      
        1 cccttgccgc gtccctcgcg aaccttccct tcaactgtaa atgtctccat gtctatttcc
       61 ccttcaataa aactttaacc gtcgtcattc tgagcgaagt gtcccgcgct ttggcatttg
      121 cgcgaagcgc cgggacacgc agccgaagaa tctctcttcg ccttcgctaa ataaataatt
      181 cgctgatcga agtctcttca tgaatcgact gtattcctcg cgccagcagc ggcgccacgc
      241 tcagcgtctt gatcttcggc aattcccgcg cggcttggct cagcggaatc gaatccgtca
      301 ccaccacctc tacgatgtcc gattctcgaa tgcgctcaac tgctggaccg gacagcaccg
      361 ggtgtgtgca ggccgcataa acctttgatg cgccattttc gagcagtgct tccgcggttt
      421 tcaccagcgt ccccgctgta tcgatgatgt cgtcgatcac cagtgcggtg cggtctttca
      481 cgtcaccgat caggtgcatc acttcgctca cgttcacgtc aatccgccgc ttatccacaa
      541 tcgccagcgg cgcatccatt cgtttcgcaa atagccttgc cctctccacg ccgcccgcat
      601 cgggcgaaac caccgtcacc ggtcctaatt gcttcttctg aaaatattcc accagcaccg
      661 gcgcggcgta caagtgatcc acggggatat taaaatatcc ctgaatctgc ggcgcgtgca
      721 gatcgagtga aagcacgcgg ctcgatcccg ccgtttccaa aagatccgcc acgagcttcg
      781 ccgaaatcgc cacgcgcggc cgatccttcc ggtcctgccg cgagtacggg taatacggaa
      841 tcaccggcgt aattcgccac gccgacgccc gcttaaacgc gtccaccatg atcagcaact
      901 gcatcaggtg aagatccacc ggattcgcgc acggctgcac cacgaacacg tccgcgccgc
      961 gcacattctc aagaatctga tagcgagttt ccccatccga gaactgcccg tgctcagcct
     1021 ttccacgcgg gacgccgaga taggagcaga tgcagtcgca cagggtggga ttcgcagtcc
     1081 cgcagaagac tttgaacttc tggtcggcca aagttctccc ctatcgggtc acactccaac
     1141 ccatcgcacg gccatacttt tccctggaaa cagtttcagt gacgaaaacc gaatcttccg
     1201 ggaacgcccg agcggctcgg cgcgcctgcg ctgggtttcg gaatactcca aacaccgccg
     1261 acccgctacc cgccagcgcg gcattcgcgg ctcctcgctt gagcaatccg tcccggattt
     1321 ctccgaggcg cggatagcgg ccgaaaacag ggccctcgaa atcgttgaag accctgttct
     1381 gccggctcca gcacagagcg cagaactcga agatattagg gggtttcgtg ctctttgtca
     1441 attccgccga catccattga taagcttctt tcgtgctcac ccctatattt cgcggcgaaa
     1501 ccacgacgat cgtttgcttc ggcagatcgt ccagcggata aatctcatcg ccacgattta
     1561 cagccagcgc ccgccctccg aagagaaaaa acggtacgtc ggcgcccaat cccgcgccaa
     1621 tcgccatcag cctatcgagc ggcaatcgct tcttcgtcag gcgcagcatt ccaatcaacg
     1681 cggctgcggc atcgcttgaa ccgcctccga ggccgcgcgc cacgggtatt cgcttctcga
     1741 gtcgcgcacg gatgccgccg cgaaacccaa tctcacatcc cagcgcatca atggcgcggt
     1801 acaccagatt gtcagaaccc gccgtcagcg ttgtgtcagt tgtctccaaa aaaatgccgg
     1861 gattagacgt tcgggaaagt tccagcgtgt cgcacagcga aatcgcctga aaaatcgtcc
     1921 gcagctcgtg gtaaccatcc ggccggcgtc ctatcacgtg taggcaaagg ttaattttcg
     1981 cgaacgcagg gagacgcact gcccgtttct tactcgaaat catgcggggg agcttactgc
     2041 ggtttcgcgg aatctgggga ggatttctgc gccagatgct tcttcagcga tctcaattgc
     2101 gcttccaatt cagttacttt gtcggcttca taatccgcgg gcaccgcctt ccgccactct
     2161 gccagcgcta gttcgaaagt ttccgccgcc cgctccttct ggccgagctt caaataaacg
     2221 tcgccaagat gactaaggat ggtgggatcg ttgccttggc ggtcgattgc cttgcgcaaa
     2281 ttctcttccg cttccggcag cttgttctgc ttgtagtaag cccatcccag gctatcgaga
     2341 tacgcgccgt tattgggctc ctgcttcagc gcccgctcga tcaacgagcc ggcttcgtcc
     2401 aagcgaatgc cgcgatcagc cagcatatag ccgtaatagt tcaacaccgg cgcattatcc
     2461 ggatttacct gcagcacctt gcggaactgt tgctcggctt gatcgaatct cttctgccgc
     2521 tcgtaaatgg acccgagcag aaaccaggct gtctccttgc cggaatctcc gtgagccatc
     2581 tgctcggcct tttgggcaga ctgctccgcg tccatgtatt ttcggccgcg ctcctgcacc
     2641 tgcgcgatgt cgacataaat ctgctggtcg ccgtcattcc ctcggagcag gccctgaaga
     2701 agctgcgtgg cagccgtggt ctcggtcttt tcaccataga gcatggccag ggtaaccgtc
     2761 atctcggggt ctttcggctc cgcatccagg gccttcttag ccgcggcgat tgcctcatcg
     2821 atattgtggt tctctcgata agtttcaatc gtaagcgtct ctgcccgctt ccgcgaatct
     2881 ccgccaagct tccccatatc cgcatacgct tcgagcgcag ccgggtaatt tcgcgcgccg
     2941 cgataggcgc ggcccagttg ctcatagaga attgccagcg cgttggcgct gcctccgccg
     3001 gactggctct ttattcctgc gatcgcatcc gtcagcagct tcactgcatc gtcgtaatgt
     3061 ccctgatcct cgtcgagcag cgcttggttg aaaagaattt cgaggctccc gggcgcgagc
     3121 tgtttcgcgc gctccaagct cgattgcgcc ttgtcaaact gacccaatcg ccgataaagc
     3181 tgcgccagcc tcagatgatt ttccgaagtt cccggctcta gctccaccag cttcttatat
     3241 tgttcgagcg cgtcggcgta cttgtcttgc tccatcagcg cttcggccaa gccgtgccgg
     3301 tgaccagcgt catccggatc gagcgccacc gccttccggt acgccgcttc ggctttcgca
     3361 tagtccttcg cttgggaata cgcgctgccc aaaagatcgt aaatatcagg cgaagcagaa
     3421 tccgtggacg cctgcgtcag cagttcgatg gcttcctgtg acttgccctc gtcaatcaga
     3481 agctggctca actgttcgag cgcggcgcta ttctcggaat ccttctgcaa aacctgccgt
     3541 agaattttct ccgcgtccga gtgatgattt tcgaagcgat atagccgcgc aagccacagc
     3601 gccgaatatg tatcgtccgg ctcgaccttc agaatcgctt ggaactgctc gatcgcctgg
     3661 gtgatgctcg cttgcggcac ttcgcctccg ctcgcttcgc ccaatgcgcg aacgtaaatt
     3721 cgcgccagca gccggtgcgc atccacgttg tccgggtcga tcttcagtgc cgcttgtgct
     3781 tcttccgtgg cctcgcgcac atgtccggat ttcgcatgga tctcggccag ccgttcctgt
     3841 atcaccgc
//
LOCUS       DATNDT010000504         3962 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2094167, whole genome shotgun sequence.
ACCESSION   DATNDT010000504 DATNDT010000000
VERSION     DATNDT010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3962)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3962)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3962
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2094167"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..304
                     /locus_tag="VN788_16465"
     CDS             179..304
                     /locus_tag="VN788_16465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22176.1"
                     /translation="MEKGRINNGTLGEVKGFLRNGDIELTNGFVIPKDCGGMTHG"
     gene            414..1218
                     /locus_tag="VN788_16470"
                     /pseudo
     CDS             414..1218
                     /locus_tag="VN788_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015891148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1882..2010
                     /locus_tag="VN788_16475"
                     /pseudo
     CDS             1882..2010
                     /locus_tag="VN788_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2194..2646
                     /locus_tag="VN788_16480"
     CDS             2194..2646
                     /locus_tag="VN788_16480"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HXU22177.1"
                     /translation="MKREYSRTAKRGLWLAGLLTLSISGSAFAQQRAIGFKKGMPFSE
                     GYVAGHTLYVAGQQGPDSHSKVTGTDIALQTTNAIAAVEKVVKEAGFQMTDIVSVTVY
                     IADLNDVPKMNEVYKKLMPDPKPARATVQVAGLIGGAKIEISAIAVKR"
     gene            complement(2822..3800)
                     /locus_tag="VN788_16485"
                     /pseudo
     CDS             complement(2822..3800)
                     /locus_tag="VN788_16485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943746.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            complement(3797..>3962)
                     /locus_tag="VN788_16490"
     CDS             complement(3797..>3962)
                     /locus_tag="VN788_16490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HXU22178.1"
                     /translation="EEILPGDVAWFSPGEKHWHGAKPTTAMTHIAIQEKLDGKVVDWM
                     EQVSDEQYKR"
BASE COUNT          852 a         1090 c         1204 g          816 t
ORIGIN      
        1 cgcataccgc agaaaatggc gagatgcgcg atgatgccct cgcgtgtgtc caagacggtg
       61 atgtactgtt cggcttcctc cttgctcatg gtcctgcgtt cggcggtctt ggcctgcctg
      121 ggcgtataga gcgcgcccgt gggatcgcgc cccacatagc cttcggcaag cgcgagcgat
      181 ggagaagggc cgtatcaata acggcactct cggcgaagtg aagggcttcc ttcgcaacgg
      241 cgatattgag cttacgaacg gatttgtcat tcccaaagac tgcggcggca tgacgcacgg
      301 ctagctcgtg acctcatacg catcgccggg ccaagacggg tggacctgcc gctcgtggcg
      361 ctgggctccg aatccttcgc agccgctaac cgcgagcagc tctatgtctc actgtcgcgg
      421 gctcaggaaa gagctcgctc atcaacgaag tgttccgtgc agcatcccga cgccatcgtg
      481 atccatcaat ctgccgtcgg aacatccact cgcagcaacc ccgcaacgta caccggcgtg
      541 aagatgatct gcgcaaggcg ttcgcgtcgg ccaacaaagt ggacgcggga ctgttcagct
      601 tcaactcgaa aggcgcatgc gaagcgtgca acggctcggg agtggtctac accgatctcg
      661 cgttcctcga tgcgttcaaa tcaccgtgca acgtgtgccg gggccgccgc ttcaaggacg
      721 acgtgctggc gtacaagctc gacgggaaat cgatcagcta cgtgctcgcg atgacggtgg
      781 cgcaggcggt ggagctgttt caaaagcgta aggagatcgt gcgcaagctg caggcgatgc
      841 acgacgtggg ggctcgcgta tctcacactc ggccagccgc tgggcacgct ttccggcggg
      901 gaataccagc gcatcaagct cgccagcgag ttgcacaagg acggcagcgt ctacgtgatg
      961 gatgaaccga cgacgggatt gcacatgtcc gatatcgcgc acctcctcgc catcctcgac
     1021 cggctcgtcg atggcggcaa tagcgtgatc gtcatcgagc acaacaggac gtcatccgta
     1081 gcggggattg gatcatcgat ctggggctgg agggcggcag caagggcggc gaggtgatgt
     1141 ccgagggtac gccgacggag ctggtgaaaa gcaaagcgct cgctgaccgg gcagtacctg
     1201 gcgaaaggcg tggcgtaaaa cttcgcgaaa aggcggcgga gaaatcgctg cggtgatctt
     1261 ttgcgagatc tttttcgcaa aacgcctcct cacgaaagct cgttcaattg ctcttcagtg
     1321 tgcttacgaa agcaggagtt caggatccca cgtcggagag cgaggtgttc ggaggcgcgg
     1381 ggcagtttgc atacgcgatt gagcaagtgc tggagcgttt ggcgacgtca gacttacggg
     1441 aggccgacaa ggcgcgcgcc gagttcgcgt ttctgccaag cctgatcgaa acaagtggag
     1501 ggcctacgct gaatgcctgc ctgtgcgagg acgccgcgtt ttttgcactc ttcggagaaa
     1561 tcggggtagc ggtttcatgc gggcgcttga gaggaagagg gaggccgagg gccttgaacc
     1621 gctggatgcc atctgctgat cggatggggt tttgcaacca tgaagcggag cttgagcagg
     1681 ggatacgaca ggttgcggta gccgcagccg cgcggcggac catgaatgaa tgctcggccc
     1741 atcctagtcg attcgggtcg acagacttgg gcgctacccg gttttcggcc atacactggc
     1801 acatggccgc acgttccgcg tgctgacgat caccgacgga tatacgcggg aatgcctata
     1861 accgtcgagg tgctgcgcta tatcgagccc ggccgcccga tgcagaacgg ctacatcgaa
     1921 cctttcaacg gccatcttcg cgatgagtgc ctgaatgtga actggttcct gagcccggcc
     1981 aatgcacgaa ctcaactcga atgctggcgc accgatgcca ttacgtcgct tctctaactc
     2041 tttgctgttg ttgtcatatg tatccacttg gatgcgtaat ccgcgcggtt gttcccgaat
     2101 agcctctatt caagaaacgt gatcgtatcg aagtgcaaca tcggaagaga aatgagaaaa
     2161 tctcttattt gcacaagagc taggagacca accatgaaga gagagtattc gcggacagca
     2221 aagcgggggc tttggctagc ggggttgctg acgctcagta tttccggcag tgcttttgcc
     2281 caacagcggg ccatcggttt taagaagggt atgcctttta gcgaaggata tgtcgctggc
     2341 cataccctct acgttgcagg gcagcaagga cctgattcgc acagcaaggt caccggtacg
     2401 gatatcgctc tacaaacgac aaacgccatc gctgcggtgg agaaagttgt gaaagaggcg
     2461 ggctttcaga tgacggacat cgtttcagta acggtttata tcgccgacct gaatgacgtc
     2521 cccaaaatga atgaggtcta caaaaagctc atgccagatc ccaaaccagc gcgagccacc
     2581 gttcaggttg cgggtttgat cggcggcgcg aagatcgaaa tctcggcgat cgcagtgaag
     2641 cgctagttta attgttttat cgatgtcccg atgcggcgga tttggcaacc ggaactgttt
     2701 tccggatggc gcataatcca gggttggccg gcaacacagt ttgagtaacg gctagccctg
     2761 acacaaatct cccgaagcgg aaacgcattc gctccatatt tctatcattc agatttgaga
     2821 gagccatatc gatggagaag cgatatttca cgtcggactt aagcagcctg tcgtaggctt
     2881 cgttaacctt ctggatcggg atgacctcga cgtccgcggt gatgttatgc tctccgcaaa
     2941 agtcgagcat ctcctgagtc tcgggaatac cgccgatgtt cgaaccagaa acgctgtggc
     3001 atcccaaaat aagtcccaac gaccagacgt ccagaggctt cgccgaacgc cgacaagagt
     3061 gagagcaggt tgatataggc gttgatgccg tgatccgccg atacggcgtc gcgaatgaaa
     3121 tcgaagctgc cggcgtgctt ttttatctcg tgcgaattcc gggaaaggac gatttcgccg
     3181 gcgccgagac gaagcgcgtc ctccttcttg ctgggcgaag tggtgaagtc cacaacatga
     3241 gatccaagcg catgcgcaaa cttcgcggcc atgtgacccc gccggccgag cccggcgacg
     3301 ccaacctttt gcccttcgta atgccccagt ggcgcatggg cgagtaggtt gtgatgccgt
     3361 cgcagagcag cggcgcggcc cggcgagatc cagatggagg gcactcgcag aacgaaaact
     3421 ctcattgatg tcgacactat cggagtagtt gaaggtgagc gtcagattcg ggcagaaatt
     3481 ctcaaggcct gccttgcacc ttgggtaggt gcggtcggag tcgatcaagc agccgactgc
     3541 ggcgaggtcg cccggcttga acttggttac tgccgagccg gccttcgtga cgcgtccgac
     3601 gattcatggc cgggcacaat cggatagcta ggcggtatga actcgctcca ctcgttgcgg
     3661 accgaatgga gatcggagtg tcagatgccg caaaagagga tttcgatctg cacgtcctgt
     3721 tcggttggat cgcgccgcgc gattgtcgta gaggcaaacc gcgacgccgc tctgcgagtt
     3781 tccttcgacc catcagtcag cgtttgtatt gttcgtcgct gacctgttcc atccagtcga
     3841 caaccttccc atcgagcttt tcctgaatgg cgatgtgcgt catcgccgta gttggcttgg
     3901 ctccatgcca gtgcttctcg ccgggcgaga accaggccac atcacccggc aggatctcct
     3961 cg
//
LOCUS       DATNDT010000505         6048 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3331211, whole genome shotgun sequence.
ACCESSION   DATNDT010000505 DATNDT010000000
VERSION     DATNDT010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 6048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6048
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3331211"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1269
                     /locus_tag="VN788_16495"
     CDS             <1..1269
                     /locus_tag="VN788_16495"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HXU22179.1"
                     /translation="GHLAAVIALAAAISSPLRAQQAKAEDYPPPKPAFPGQTNAPPPA
                     KKSPAFNVDTIIPRFMAPWSLAFMPNGKMLVSERYGFIRVASMNGFYSAPISGVPDTK
                     IVAAESLHDVVVDPDFAHNRMIYFTYFAPPPGEAPGVWPLDVFYGNVWKASVSERRTM
                     RVGEERVARARLSEDEKRLENVEVLLDGVNRRIVFAPDGTLFVTGADRFRFYEPGYDG
                     TNHEITDPDILRNFTGRVVHINRDGSIPKDNPFLDQPTVLPETYSYGHRDPEGAAINP
                     ATGKLWVIEHGPLGGDKISIIKPGGNYGWPNVSYGRRYNGDPIAGTGATQKPGVEQPV
                     YFWYPDIAPCGMMFYTGDLFPEWKGNLFVAGLLARTLIRLELDGNRVVAEERLLHDLN
                     ERIRDVQQGPDGAIYLLTDDGKLLRLTPKK"
     gene            complement(1666..3288)
                     /locus_tag="VN788_16500"
     CDS             complement(1666..3288)
                     /locus_tag="VN788_16500"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HXU22180.1"
                     /translation="MTRSIKNFKFAAFLAVLAFAYSVAAQTPTPVRTQAGLVQGKMEG
                     DVTVYKGIPFAAPPVGDLRWRAPQPPAAWKGVHAADKFAPACMQTPVVMPALGLDALP
                     VSEDCLYLNVWTPAKSPKDHLAVMVWIYGGGFSIGGTSLRQYDGEYLAKKGVVLVSIA
                     YRLGPFGFLATPDLSAEDHGQSGNYGMLDQIAGLEWVKRNIAAFGGNPNRVTIFGESA
                     GGIAVSMLAASPLAKGLFQGAISESGGSFAPARFSNEGGENVPPLSVAEKHGTSFLAK
                     ISAKSIADARKMTAEAILKASPPGTGGGFWPNLDGYVLPGDQYNLYEEGRYNDTPILI
                     GTNSDEGALFVATTTAPAYEKSVHAGYGDYADKILAAYPSGSDAEALRSARDLARDST
                     FGWGTWTWARLESKRSKSKVYVYYFSHRPPYPDTPQFKSWGAAHGAEISYVFGNFTPS
                     MPPTNEDRAVSDTVSSYWVNFAKTGNPNANGMPVWPAFSNAKPEVMNLNDPSKAIPLP
                     NADKLKVLDGYYAWRRSQEAEKLSSSSPKGAN"
     gene            complement(3343..3849)
                     /locus_tag="VN788_16505"
     CDS             complement(3343..3849)
                     /locus_tag="VN788_16505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006960161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HXU22181.1"
                     /translation="MPKTAPLTVEDRIAINDLLAEYAWALDTGDVDALITCFTPDAVV
                     IEEVFEEPDRWEGLENIRRFAEHFRNVPNFPGRQHHISQLLVKGNSKLCSARSFVFVT
                     ECQGEPPYTIRFAGYYDDHLVKSGGKWFFRQRVIRLWDGEVLAGFPGRGKRTPRKRPP
                     ELVIKKAV"
     gene            complement(3853..4926)
                     /locus_tag="VN788_16510"
     CDS             complement(3853..4926)
                     /locus_tag="VN788_16510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22182.1"
                     /translation="MKQPALGIVASFLVIVISLGFIALFTFPIFANWVAFLTICFIPM
                     EIVISVTWGGNYPAFAAARPQPAKGILLTLLALAVGAVVAVVHFLTVGGGVNPPPPMV
                     AMCIIASVVITFWLAIMWAGWPFTRLIKNPVAAGVVMLVACYLINYLLFRLLFDYGFM
                     RGAPVYVPSLDPHGLFNAWSVLVFYITVIAVMFVSLNFDMWPFTKYPALMKQPLLGLV
                     WTFTILVIGGLAFYIGVYLLKMDVVSFMVRVPIPFVFGTIIVLNMMHGSIFGKLTQPL
                     KGVLNTVASAAIGTVLAIVYGALAPLVTGKVNPGPPAYDFEIWLASALLAVTFPFLIL
                     HAEFFKMWPLHRERAQSSAPRVE"
     gene            complement(5001..5657)
                     /locus_tag="VN788_16515"
     CDS             complement(5001..5657)
                     /locus_tag="VN788_16515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017361370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HXU22183.1"
                     /translation="MKIALIGASGFVGSKILAEALQRGHQVTAIVRNPEKIQPQENLT
                     VKKIDVLQTDELARLLAGNDAVISSFNPGRGVAGAEVYDKFVQGHKSIIAAVKKSGVK
                     RFLGVGGAASLKTAEDIEFLDSPTFPKEYEPYKPGIRGTRELYYLLKQEPTLDWIFLA
                     PSSQITPGERTGKFRLGKDHLLFDEKGASKISLEDYAMAMIDELERPQHHRERFTVGY
                     "
     gene            5764..>6048
                     /locus_tag="VN788_16520"
     CDS             5764..>6048
                     /locus_tag="VN788_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014077101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HXU22184.1"
                     /translation="MIESAPFGSAQSLLHEPKLEFAFEVRLRFTRVQNIANMPTGAGR
                     GAVYVDSGEFSGPRLRGKAVPNSGGDYSLFRPDGVLAFDARYMLQEDDGTL"
BASE COUNT         1348 a         1741 c         1722 g         1237 t
ORIGIN      
        1 gggcatttgg ccgccgtgat cgcattggct gcggcaattt catcgccgct ccgcgcgcag
       61 caggccaaag ccgaagacta cccgccgccc aagcccgcct ttcccggcca gacgaacgct
      121 ccgcctcccg caaagaaatc accagcgttc aatgtcgata cgatcatccc gcgattcatg
      181 gcgccgtggt cgctcgcctt catgcccaac ggaaaaatgc tagtcagcga gcgttacggt
      241 tttatccgcg tcgcctcgat gaacggattt tattccgcgc ccatctctgg cgtgccggac
      301 acgaaaatcg tcgccgctga atccttgcat gacgtggttg tcgatcctga cttcgcccac
      361 aatcggatga tctatttcac ctacttcgcg ccgccgcccg gcgaagcgcc cggcgtctgg
      421 ccgctcgacg tcttctacgg caacgtctgg aaagcctccg tctccgaacg ccgcaccatg
      481 cgcgtgggtg aagagcgtgt agcccgcgcg aggttgagcg aagacgaaaa acggctcgaa
      541 aacgtggaag tgctgctcga cggcgtgaat cgccgcatcg tctttgctcc cgacggtact
      601 ttgttcgtca ccggtgcgga ccgcttccgc ttttacgaac cgggttatga cggcacgaac
      661 cacgaaatca ccgaccccga tattctgcgc aatttcaccg ggcgcgttgt gcacatcaat
      721 cgcgatggct cgatccccaa ggacaatccg tttctcgatc aaccgactgt cctgcccgaa
      781 acatattcct acggccatcg cgatcctgaa ggcgcggcga tcaatccggc caccggaaag
      841 ctctgggtga tcgagcacgg cccgctcggt ggtgacaaaa tcagcatcat caagccgggc
      901 ggcaactacg gctggcccaa cgtcagttac ggccgtcgct acaatggcga ccccatcgcc
      961 ggtactggcg cgacgcaaaa gccgggcgtc gagcagcccg tctacttctg gtatccggat
     1021 attgctccct gcggaatgat gttctacacg ggagaccttt ttccggagtg gaaaggaaac
     1081 ctgttcgtcg cgggattgct tgcgagaact ctaattcggc tcgagctgga cgggaatcgc
     1141 gtggttgccg aggagagact acttcatgat ctcaacgagc gcattcgcga tgtccagcaa
     1201 ggtcccgacg gcgcaatcta cctactcacc gacgacggaa agctcctgcg gctcacaccg
     1261 aagaaatagc tcgctgccac ttcggcgccg tcagtccgag cgtccggcag ccaagtggcg
     1321 gacacgcgaa actgacccgc tgctgaaagt ccacaggagt cgcttgacaa aatgaaattc
     1381 atctcatgct aatttgaaat cgggcgcgct gcttgcgcca gaaaggaagg actacggagc
     1441 ttgttcacgg caatcacgta tcgcagcgag aggtgggaca gacgggccga cctgtcagct
     1501 tgatgcccca ggagaggctg ggatcgtcaa tttgccgcca tccatcccaa ctagccgcac
     1561 gtaaagctga agggtctaat cgacagatgg gacgagtaga acatgatcca actctatgaa
     1621 aacaaagagt tgtggccagt tctaatcgac acaaatccgg agacgttagt tggctccttt
     1681 gggcgaacta ctcgagagct tctctgcttc ctggcttcgc cgccaggcgt agtagccatc
     1741 cagcacttta agtttgtccg cgttgggaag cggaatcgct ttcgatggat cgttgagatt
     1801 cattacttcc ggcttggcgt tgctgaacgc aggccagacg ggcataccgt tcgcattcgg
     1861 attgcctgtc ttagcaaaat tcacccagta agaagaaacc gtgtcggaga cagcgcggtc
     1921 ttcgttagtg ggaggcatgc ttggagtgaa gttgccaaac acgtaggaaa tttccgcgcc
     1981 gtgcgcagcg ccccagcttt taaattgcgg cgtgtcggga taaggcggcc ggtgactgaa
     2041 ataataaacg taaactttgc tcttgcttct cttcgattcg agccgcgccc aggtccaggt
     2101 gccccagccg aacgtggaat cgcgggcgag gtcgcgggcg gaacgaagcg cttcagcatc
     2161 ggaaccactg ggataggcgg ccaatatctt atccgcgtaa tcgccgtatc cggcgtgaac
     2221 gcttttctcg taagccggtg cggtggtagt tgcgacgaag agtgcgccct cgtccgaatt
     2281 agtgccaatc aaaataggag tgtcgttata gcgtccttct tcgtagaggt tgtactgatc
     2341 gcccggaagg acgtagccat caaggttggg ccagaaaccg ccgcccgttc ccggcggcga
     2401 agccttgaga atggcctcgg cggtcatttt tcgcgcgtct gcgattgatt tcgcgctgat
     2461 cttggcgaga aaagatgtcc catgcttctc ggccacggag agtggaggca cgttctcgcc
     2521 gccttcattc gagaagcggg ctggcgcgaa ggatccgccg ctttcggaga tcgcgccctg
     2581 gaaaagtccc ttggcaagcg gcgaagctgc cagcatgctc acggcgattc cgccggccga
     2641 ttcgccgaaa atcgtgacgc gatttggatt tccgccgaat gccgcgatgt tgcgcttcac
     2701 ccactcgagc ccagcaatct gatcgagcat gccgtagttc cccgactggc cgtgatcctc
     2761 cgcgctcaga tccggagtcg cgaggaagcc gaacggccca agcctatagg cgatgctgac
     2821 cagcaccacg cctttcttcg cgaggtattc gccgtcgtat tgccggagag aagtgccgcc
     2881 gatcgaaaag ccaccgccgt aaatccagac catcacggca agatgatctt ttggagattt
     2941 cgccggggtc cagacgttga ggtagagaca atcctcgctc acaggtaggg cgtcgagccc
     3001 gagcgctggc atcacgacgg gagtctgcat gcaggccggc gcgaatttgt cggctgcgtg
     3061 cacgcccttc cacgcggcgg gtggctgtgg cgcgcgccag cgcaaatcgc ccactggcgg
     3121 tgcggcaaac gggatgcctt tgtaaaccgt cacgtcgcct tccatttttc cctggaccaa
     3181 ccctgcttgc gtgcggactg gcgtgggcgt ctgcgccgcc accgagtacg caaatgccag
     3241 aaccgccaag aatgctgcga atttgaaatt tttgatactc ctcgtcatct ttctcctccg
     3301 cacagcagtc aagctatttc cggctgccgc aaaataaacc aactacacag ccttcttgat
     3361 cactaattcc ggaggacgct tgcgcggtgt tcgcttaccg cggcccggaa aacctgcgag
     3421 cacttcgcca tcccaaaggc gaatgacgcg ctgccgaaaa aaccatttgc cgccggattt
     3481 cacgagatgg tcgtcgtaat agccggcgaa gcggatcgtg tatggcggct cgccctggca
     3541 ttcggtgacg aaaacaaatg atcgcgccga gcacagtttg ctatttcctt tgacgagcag
     3601 ctggctaatg tggtgctgcc gtccaggaaa gttgggtacg ttccggaaat gttccgcgaa
     3661 tcgccggata ttttcgaggc cttcccagcg gtccggttct tcgaatacct cttcgatgac
     3721 aactgcgtct ggagtgaaac acgtaatgag agcatccaca tcaccggtgt cgagcgccca
     3781 cgcatattcc gcaagaagat cgttaatcgc gatccggtct tcaaccgtta acggcgccgt
     3841 tttcggcata gattactcga ctcgtggcgc agacgattgc gcgcgctcgc ggtgaagagg
     3901 ccacatcttg aaaaactccg cgtgcaggat gaggaaggga aaagtcacgg ccagcagcgc
     3961 cgatgccagc caaatctcaa aatcgtaagc aggcggaccc gggttcactt tgcccgtcac
     4021 caagggagcc agggctccgt aaactatcgc caggacagtc ccgattgcag cggacgcaac
     4081 tgtgttcaaa acacctttca gcggctgagt cagtttgccg aatatcgatc cgtgcatcat
     4141 gttcagaacg ataatcgtgc cgaagacgaa gggtatggga acgcgaacca taaacgacac
     4201 cacatccatt ttcagcaggt acacgccaat ataaaaggcg agccctccga ttaccagaat
     4261 cgtgaaggtc cagacgaggc ctaggagggg ctgtttcatc agcgccggat atttcgtgaa
     4321 aggccacata tcgaagttga gtgacacgaa catgaccgcg atcacggtga tgtaaaacac
     4381 cagtacactc caggcgttga ataatccgtg gggatcaagg gacggtacgt acacaggcgc
     4441 gccccgcata aaaccgtagt cgaacagcag tcggaacagc agataattaa tcaagtagca
     4501 cgccaccagc atgacgacgc cggcagcaac tgggttcttg atcaatctcg tgaagggcca
     4561 gccggcccac atgatggcga gccaaaacgt aatgacaacc gatgcaatga tgcacatggc
     4621 caccatcggc ggaggcgggt tgacgcctcc gcccaccgtc aggaagtgga ccaccgcgac
     4681 gaccgctccg acggccaggg ccagtagtgt cagcaaaatg cccttggcag gttggggacg
     4741 cgctgcggca aacgcgggat agttaccgcc ccaggttacc gagatcacaa tttccatggg
     4801 gatgaagcaa atcgtcagga aggcaaccca gttggcgaat atggggaacg taaagagcgc
     4861 gatgaaacca agcgagatta caattacgag aaatgaggcg acgatcccaa gcgcgggttg
     4921 tttcactcca ccctccttct tcctcgacgc gaacggttgc tgcgggccct ggaaactctt
     4981 cgcatccgtg aatcgagact tcaatatccg acggtgaatc gctcgcgatg atgctgcggg
     5041 cgctcgagct cgtcgatcat ggccatggcg taatcttcga gagagatttt gctggcgcct
     5101 ttttcatcga agaggaggtg atccttgccc agacgaaact tgcctgtgcg ttcgcccggc
     5161 gtaatctgcg aggagggcgc gagaaaaatc caatcgaggg tcggttcttg cttcagcaaa
     5221 taataaagct ctcgggtacc gcggattccg ggtttatagg gttcgtactc ttttggaaac
     5281 gtcggagaat cgagaaactc aatgtcctcc gcggttttca gactggccgc gccacccacg
     5341 ccgaggaatc gcttgacacc ggatttcttc acggcggcga taatcgactt gtggccctgc
     5401 acgaatttgt cataaacctc cgctccggcg acgccgcgtc caggattgaa agagctgatg
     5461 accgcatcgt tgccagcgag caggcgtgcg agctcatcgg tctgtagcac gtcaattttt
     5521 tttaccgtga gattctcctg cggctggatt ttttcaggat tgcgaacgat cgctgtgacc
     5581 tgatggccgc gttgcagcgc ctcggcgagg attttcgagc ctacaaatcc gctggcgcca
     5641 ataagcgcga ttttcatgag gatccccccg ggatctaacg gcaagccagt tacgacgatt
     5701 gacaggttta aagaacggcg ggattgtaca ccttcatatg cctgggattc acggagatat
     5761 cgcatgatag agagcgcgcc ctttgggtcc gcacaatcgc tgctacatga gcctaaactc
     5821 gaattcgctt tcgaagttcg cttgcgtttc acgcgcgtcc agaacatagc gaacatgcca
     5881 actggcgcag ggcgcggagc agtttatgtg gatagcggag aattttccgg cccacgcctg
     5941 cgtgggaaag ccgtgccgaa tagcggcgga gattattcgc tgtttcgccc cgatggcgtg
     6001 ctggcgttcg acgcgcggta catgctgcag gaagacgatg gcacgctg
//
LOCUS       DATNDT010000506         3767 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3598810, whole genome shotgun sequence.
ACCESSION   DATNDT010000506 DATNDT010000000
VERSION     DATNDT010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3767
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3598810"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(317..889)
                     /locus_tag="VN788_16525"
     CDS             complement(317..889)
                     /locus_tag="VN788_16525"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HXU22185.1"
                     /translation="MPVVISMLRGVNVGGHNKIKMDALRALYESLKFTGCQTFIQSGN
                     VIFKTKERNLAKLTSQIQTAIERSFGFRPGVILRTADELRDVIARNPFAKRRGIEPGK
                     LLVTFLASAPTTQARDNLLKLKTDGEELRIDGRELYTYFPNGQGRSKLTWPIIERTLK
                     VSGTGRNWNSVTKMLAIADALAADTGSAKG"
     gene            complement(1178..2923)
                     /gene="rmuC"
                     /locus_tag="VN788_16530"
     CDS             complement(1178..2923)
                     /gene="rmuC"
                     /locus_tag="VN788_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015437855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HXU22186.1"
                     /translation="MAGHPYLIFYSGLIIVFLLAATLWLISRKPKGSASKGDKEGQQI
                     EIARLNERNTSLIDEVGKCRAKISEWEAKDQDWQKEISIARDDRSRFEERASRVAPLE
                     VELSQTKRLVDSLQEKNITAETQLAERTQSLDYAQKQRLPELESLLSQARTEVSTLRE
                     NLARITSTCDQTKAVVEALTAELSEVKPKYERLREDNEHIKTEFAQVTTTLNNERDKN
                     TEKLALLTEAKEQLTNQFKSLANDILEEKSKRFTEQNKQNLGQILDPLKDKLKSFQEK
                     VEQVYIQEGKDRTALSEQVKQLLALNQQLSADAGNLTQALKGSSKAQGTWGELVLERI
                     LETSGLRKGHEYDTKESHVREDGTRAQPDVVIHLPEDKHLVVDAKVSLTAYEDYAKGQ
                     DDASRNSATARHLTSVRAHVKDLSGKNYQQLYGLKSLDFVIMFIPIESAFILAINEDS
                     KLWEDAWKRNVILISPSALLFVLRTVAHLWRQEQQNQNVQDIAKRGAELCDKLAGFLE
                     DLDGLGSRMNQARESYDNAMNKFTRGRGNLIWQAQRLKDLGVKPSKPLPHALIESASP
                     EDLEILPDSSESTLG"
     gene            complement(3026..>3767)
                     /locus_tag="VN788_16535"
     CDS             complement(3026..>3767)
                     /locus_tag="VN788_16535"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HXU22187.1"
                     /translation="TGNIGKAKTYVVEGIGEDIFPSTMDFKVLDDILQITDEESFVWA
                     RRLAKQEGVFTGGSGGGCVSAALRLARECKKGDVVVAFLPDTGMRYLSKVYNDGWMRE
                     HGYVESEVPLCAADVVRVKLQNGKHRELVIARPYQTVFHALHIMQQQDISQLPIFEDD
                     NPIGTIYEDQILNLALQGKDLRKLVIREVMAEALPRVPSDSPVERVTHLLSHSGPAVF
                     VEMSNGKFEILTKFDLMDTIAGIMEQKR"
BASE COUNT          716 a          928 c         1120 g         1003 t
ORIGIN      
        1 ggaccgcccc caggccattt atccggacgg tgccgcggga gcgttggcac aacttgaatt
       61 cattaccgat caagaaaatg taaaccgttg gatataaggg acttatgcaa acgcaactgt
      121 gcatttcatt accgatcaag attgggcgtt ttcggagtgg ttttggcggt tctgaggtgc
      181 gtcttggtct gttatacgtc ggggctgctg gcatcgtcga attttcgctc aagcaaggct
      241 agcacgatgt gtcgcgcagc gatagttgat gtggctgtgg aaacgacggc taacagcaaa
      301 acaggcggga tttgcgctac cccttagctg aaccggtgtc ggcggcaagc gcgtcggcga
      361 tggcgagcat ttttgtgacg ctgttccagt tgcgcccggt gccggagact ttgagcgttc
      421 tttcgatgat cggccaggtg agtttcgagc ggccctggcc gttgggaaaa taagtgtaaa
      481 gctcgcggcc gtcgatgcgt agttcctcgc cgtcggtctt gagcttcaga aggttatcgc
      541 gagcttgcgt ggtcggtgcg ctggcgagga aagtgacgag gagtttgcct ggctcgatgc
      601 cgcggcgttt ggcgaaagga tttctggcga tgacatcgcg cagttcgtca gcggtgcgaa
      661 gaatcacgcc ggggcgaaag ccgaagctgc gttcgatggc ggtttgaatc tggctggtca
      721 gtttcgcgag gttgcgctct ttcgttttga aaatgacgtt gccgctttgg atgaaagtct
      781 gacagccggt gaatttcagg gattcgtaga gcgcacggag cgcgtccatt ttgattttgt
      841 tgtggcctcc gacgttgacg ccgcgcagca tggagatgac gacgggcatg ggtgggagta
      901 tagcgcgggg aagggcagtg gctagtggtt agtggcgagt ggctagtgaa ggcgaagact
      961 tcgcgctctt ccttcgtcag ggtaaacgga tgaagcgcgc gaaaggaaat atcttttcca
     1021 gttcgtttac ggcacggccc ttcgtgcctc agggtgaact gaagcctgcc tgcgcaggca
     1081 ggccgtgccc tgccaagaga atcgaagctg ccacgtagac tctgaaagcg gtcgcgatcg
     1141 gttgggcgct tggttttggt acgctggccc aagacttcta gccgagtgtc gattcgctac
     1201 tgtcgggaag gatctccagg tcctccgggg acgcggattc tatcagtgcg tggggcagag
     1261 gcttgctagg tttcacgccg aggtctttga gtctctgagc ctgccaaatt agatttcctc
     1321 tgccgcgcgt gaacttattc atggcgttgt catagctctc tcgcgcttgg ttcattctgc
     1381 tccccagtcc gtcgaggtct tctaggaagc cagccagctt gtcgcataat tctgctccgc
     1441 gcttcgcaat gtcttgaaca ttttgattct gctgttcttg tctccacaga tgtgccactg
     1501 ttcgaaggac gaagagcaag gcgctggggc tgattagtat gacatttctc ttccaagcgt
     1561 cttcccacaa tttactatct tcattaatgg caaggataaa ggcagactcg atcggaataa
     1621 acatgatcac aaaatctagg gatttcaacc cgtagagttg ctggtaattc ttgcccgata
     1681 aatctttgac gtgggcgcgc actgacgtga gatgccgcgc cgtagcggaa ttccggctag
     1741 catcgtcttg acctttcgcg tagtcctcat aggctgtaag ggacaccttt gcgtcaacca
     1801 cgagatgttt gtcttcaggg aggtgaatca ctacgtccgg ctgggctcgc gtgccgtcct
     1861 cgcggacatg gctctccttg gtgtcgtatt catgcccttt acggagtccc gatgtttcga
     1921 gaatgcgttc gagaaccagc tctccccagg ttccctgggc tttcgaggat ccctttaagg
     1981 cttgggtgag gttgccagcg tcagctgata actgttgatt gagcgccaag agctgtttga
     2041 cttgctcgga gagtgctgtg cggtctttgc cttcttggat gtacacctgc tcgacctttt
     2101 cttgaaagct cttgagctta tccttgagag gatcgagtat ttgtccgaga ttctgtttgt
     2161 tttgctcggt gaatcgcttc gacttttcct ccagaatatc attggcgaga gacttgaact
     2221 gattcgtgag ttgctccttg gcttccgtga gtagcgccag tttttctgtg ttcttgtctc
     2281 gctcattgtt tagcgtggtc gtcacctgag cgaattccgt tttaatatgc tcattgtctt
     2341 cgcgcagtct ttcgtacttt ggcttgacct cggataattc agctgtgagg gcctcgacga
     2401 cagctttagt ctgatcacat gtcgaggtga ttctcgcgag gttttcgcgc aaagtcgaca
     2461 cttctgttct ggcctgtgaa aggaggctct ctaactcggg caatctttgt ttttgtgcgt
     2521 agtcgagaga ctgggtgcgc tcggccagtt gggtctcagc agtgatgttt ttttcctgga
     2581 gcgaatctac cagtcgcttc gtttggctca attctacttc gagcggggcg acacggctgg
     2641 cgcgttcctc aaagcgagat cggtcgtcgc gggctattga gatttccttc tgccaatctt
     2701 ggtctttagc ctcccattca ctaatcttgg cacgacattt ccccacctca tcaatgagtg
     2761 aggtgttacg ttcgtttagg cgtgctattt caatctgttg cccctctttg tcacccttgc
     2821 ttgccgaccc tttgggtttt cgtgaaatta gccaaagtgt cgccgccagg agaaacacga
     2881 ttatcaggcc agaataaaat atcaagtacg gatgtcccgc cacggtcctg cccccactct
     2941 tgcagtgatt taattaagga gtactgtacc acaatagaac tgcggggatg tcaatactcg
     3001 cgtagagagt ggtgatccag agaactcagc gcttttgttc catgatgccg gcgatggtgt
     3061 ccatgaggtc gaatttggtg aggatttcga atttgccgtt ggacatttcg acgaagacgg
     3121 cgggaccgga atggctgagg agatgcgtga cgcgttcgac gggagagtcg ctggggacgc
     3181 gcggaagggc ttcggccatc acttcgcgga tgacgagttt gcgaaggtct ttcccttgca
     3241 gcgcgaggtt cagaatttgg tcctcgtaaa tcgtgccgat gggattgtcg tcctcgaaaa
     3301 tcggcaactg ggaaatgtcc tgctgctgca tgatgtggag cgcgtgaaaa acggtttggt
     3361 acgggcgggc gatgacgagc tcgcgatgct tgccgttctg caatttgacg cggacgacgt
     3421 cggcggcgca gagaggaact tcggattcga cgtagccgtg ctcgcgcatc cagccgtcgt
     3481 tgtagacttt gctgaggtag cgcatgcccg tgtcggggag gaaggcgaca actacgtcgc
     3541 cttttttgca ctcgcgcgcg agacgcagcg ccgctgagac gcatccgccg ccggagccgc
     3601 cggtgaaaac gccttcctgt ttcgcgaggc ggcgcgccca gacgaaagat tcctcgtcgg
     3661 tgatttgcag aatgtcgtcg agaactttga aatccatcgt cgaggggaaa atatcttcgc
     3721 cgatgccttc cacgacgtag gttttcgcct tgccgatgtt tcccgtc
//
LOCUS       DATNDT010000507         5848 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2434072, whole genome shotgun sequence.
ACCESSION   DATNDT010000507 DATNDT010000000
VERSION     DATNDT010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5848
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2434072"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1915
                     /locus_tag="VN788_16540"
     CDS             <1..1915
                     /locus_tag="VN788_16540"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HXU22188.1"
                     /translation="AGISFEGNKYFNSDLLSRRLALQPASFASSGRFSQQLLRGDTDS
                     IRAVYLSNGFQDVQVSSSVDEHHRGKKNNLFVSFTIVEGAQTRIDGLRIEGNHAITTP
                     DLLSVIGSTHGEPYSEAGAASDRNNILAFYYNEGFPQASFRSEVTPAGATHEVNLVYH
                     ITEGPRVDVAKVLITGYQYTRPGIIRRQVAIEPNGPLREGDIGTTQRQLYNLGIFNRV
                     QIAPQNPDGTDPDKTVVVEVQEGDRYTIGYGFGFEVQRIAGGTSNPNGTTLGASPRGI
                     FEISRNNMFGRAQTISFKARASTLEYRFALGYTANNFLTNRKLSLGLTGYADKTQDIN
                     TFTSTRYEGGLQVVYKISPSSSLLFNYFYRRVEASNLVKTINVEQIPLLSQPTRVSGF
                     GVTYARDRRNNPADASRGTFNTIDVSDAIQSIGSSASFLRVYMQNSSFHSFGRAFVFA
                     RSVRFGFEQPFGDTLGETPVECTSTPVSTTQSIIPLPERFFAGGGTSLRGFGLNQAGP
                     RDPCTGFPIGGLALLIFNQELHFPMRLPFVGNRLGGTIFYDGGNVYSDTGHISFSWKA
                     PSFTDLNYFSHTVGFGLRYPTPVGPVRIDFGYQLNPARYQGIVFPSGSTVGVSKILQL
                     PHFGFFFNIGPIF"
     gene            1915..2619
                     /locus_tag="VN788_16545"
     CDS             1915..2619
                     /locus_tag="VN788_16545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22189.1"
                     /translation="MRGANFICVLMMGLAGLPAAGAARMAMPVQTASSASSAAQGRRQ
                     TMDRIAARIEDDIIMESDVRELAGFQQLVDGHSKSRDELIRELAGEWIVRGEADAAKY
                     PQPTNDDIENAYARLAAQFASPEEFQKRMAKAGLSEAAVRRELGQQLYLSRFLDYRFR
                     PSAQVDQKQIETYYSQEFVPQLKARGQAVPPLDAVADTIREVLVQRAISDRATQWIDE
                     TRAGLKIDVISDGGQP"
     gene            2616..>5848
                     /locus_tag="VN788_16550"
     CDS             2616..>5848
                     /locus_tag="VN788_16550"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HXU22190.1"
                     /translation="MSVSFKRLSAAALILITLFILLVAIILKSGFAERWIRREIMSRI
                     EQSTGARVEIGAFHLNLWQLKFEIDDITVHGLETTDMSPLFHADRVDVGLHIISLLGR
                     KVALDELLVERPQVAVWIDREGRSNVPTPRARSTNRPWRQTLFNLRIRHLELKDGTVS
                     YNNRRTPLAVDGGNLDFVLHYDAPATGADSYAGSLDWRDVRVAAKRDVPFPFDFSAKF
                     LLRRDSFEADELIVKVLHSQLSLRADLASFARADWNIKYRGRLDLADIRHIFRSPHTP
                     GGHVEFSGQARYAAAGLNPNRVPGAGWTSSGYYGSRDISLPYTYFHESGVQSDGNFEV
                     ANGHLVVPNVRVHNLGGTTSGRLEMDFAGLVFHTKTQMRGVSVPRLFDALDNPKLPVR
                     SLHWDGAMEVDSVNTWHANFKDFRTTGEMRWSPPKVQVSGKIPATARVEFDYRSDRET
                     VAIRQSEISTPTAIVGLDGVLSADDSALEVQFHSSDLTVWDDFISAIRGPKAGSHRIA
                     GEATWTGRILGPLTGSTFAGHLSSHGTQYDQLTWDELDGDMEYSPDGFILKNSVMRRG
                     SAAATVNLSLEFDGDWNFLPTNSWMLNARIEHASADDMQAILKTGYSVSGSLTGEVKG
                     SGTRAAPVLDAAIMAEAINVDGWSFNHFEGQLHWDHDEMRLSHAQLVGDFGTFAGDIF
                     YRTQEEEAEFNVSGTGISLDKVKELQSVSMPIRGQADFALRGNGPLRSPVAQGNFHIR
                     NLTVGTEMQGDFSGQLASDGKTAHLTLASQPAESRLHGDVSIGLSGDQPVSGRLSIEQ
                     FDLDPFISAGLHIHKLTGHSTADGVFSISGALRRPDTFEMDADISRISFTYEFVQLTN
                     DGDIRLAYRRDAIRVEQAHFHGPNTDLQIGGSARFSPSSRLDFTLSGGVNLRLLGEVL
                     PELDAEGRADANMSVQGSIAQPIIVGRAKLRDASAHYADFPAGLSHVDGDIVFNKSSL
                     SFEQITAQSGGGHLTLSGNVAYGQGPLHYQVTVATSTVRIRYPTGMSWLAGGTVQLSG
                     TSSAALLSGRIEVQRLLFAQGVDMASFFASTSDTTPRAGSPSPFLQNL"
BASE COUNT         1225 a         1621 c         1719 g         1283 t
ORIGIN      
        1 cgccggcatc tcctttgagg ggaataaata cttcaacagc gacttgctct ctcggcgact
       61 ggcgcttcag ccggcttcgt ttgcctccag cgggcggttc agccagcaac tcctgcgagg
      121 cgatacggac tccatccgag cggtgtatct atcgaacggt tttcaggacg ttcaggttag
      181 ctccagcgtg gatgaacatc accgcggtaa gaagaataac ctctttgtgt cattcacaat
      241 tgtcgaggga gcccagaccc gcatcgacgg ccttcgaatt gaagggaatc acgcgatcac
      301 aacgccggac ttgctcagcg tgatcggctc gacgcacgga gagccgtatt cggaggcggg
      361 agcggcgagc gaccgaaaca acatactagc gttttattac aatgaaggtt tccctcaggc
      421 gagcttccgc agcgaggtca cgcccgcagg agcgacgcac gaggtgaatc tcgtttacca
      481 cattaccgag ggacctcgtg tcgacgtcgc aaaagtactg attacaggct atcaatacac
      541 ccgcccggga atcatccggc ggcaagtagc tatagagcca aatggccccc tgcgggaggg
      601 cgatatcgga acaacgcagc gccagcttta caacctggga atattcaacc gcgtgcagat
      661 cgcgccgcag aacccggacg gtaccgatcc cgacaaaaca gtggtggtgg aggttcagga
      721 aggggatcgc tacacgattg gttacggctt tggattcgaa gttcagcgca tcgcgggagg
      781 cacatcgaat ccaaacggca cgacgctggg cgcaagtcct cgcggtattt tcgagatatc
      841 cagaaataac atgttcggcc gggctcaaac gatttcattc aaggcgcggg ccagcactct
      901 tgaatatcgc ttcgcgcttg ggtacacggc gaacaatttc cttaccaacc gaaaactgag
      961 cctgggctta accggatatg cagacaagac ccaggacatc aatacgttca cgtccacgcg
     1021 gtacgagggc gggttgcagg tggtgtacaa aatctcgcct tcgagttcct tgcttttcaa
     1081 ctatttctat cgccgggtgg aagcatccaa tctggtgaaa acgattaacg tggaacagat
     1141 ccctttgctt agccaaccca cacgcgtttc aggcttcggg gtaacgtatg cgcgcgaccg
     1201 ccgaaataat cccgcggacg ccagccgggg gacgttcaac actatcgatg tgagcgacgc
     1261 gattcagtcg atcggttcaa gtgctagttt tttgcgtgtg tatatgcaga actcgtcgtt
     1321 tcactccttc ggccgtgcct ttgtgttcgc ccgttcggtg cgtttcgggt ttgagcagcc
     1381 cttcggcgat acattgggcg aaacgccggt ggaatgcact tcgactcccg tcagcacgac
     1441 ccaatcgatc attcccctcc cggagcggtt cttcgccgga ggcggaactt cgctgcgagg
     1501 attcggattg aaccaggccg gtcctcgcga tccgtgcacg ggattcccga ttggggggct
     1561 ggcgctgctg attttcaatc aagagcttca ttttccgatg agacttccct tcgtagggaa
     1621 ccgcctgggt ggcacgattt tctacgacgg cggcaacgtc tatagcgaca cgggccacat
     1681 ctcgttttcc tggaaggcgc catcctttac agacttgaat tatttttcgc ataccgtggg
     1741 ctttggcttg cgttatccga cgccggtcgg gcctgtgcgg atcgacttcg gatatcagct
     1801 taatccggcg cgataccagg ggatcgtctt tccttccggt tccaccgtgg gcgtgagcaa
     1861 gattctccag cttccgcact ttggattctt tttcaatata ggaccgatct tttgatgagg
     1921 ggcgcgaact tcatttgcgt actgatgatg gggcttgccg ggcttccggc ggcgggcgcg
     1981 gcgcggatgg cgatgcccgt tcagactgcc tcttcggcct ccagtgccgc gcaagggcgg
     2041 cggcagacca tggatagaat cgccgcgcgc atcgaagacg acataatcat ggagagcgat
     2101 gttcgcgagc tggcaggatt tcaacagctc gtggatgggc attccaagtc ccgcgacgaa
     2161 ttaattcgag agctggccgg cgagtggatc gttcgcggcg aggccgatgc ggcaaaatat
     2221 ccgcagccga cgaacgacga tatagaaaat gcttacgcgc ggctggcggc gcaatttgcg
     2281 tcgcctgaag aatttcagaa gcgaatggcc aaagcaggac tatcagaggc ggccgtgcgg
     2341 cgagaacttg gccagcaatt atatctctcc aggtttcttg actaccgatt ccgtccatcc
     2401 gcgcaggtag atcaaaaaca gatcgagaca tattacagcc aagaatttgt gccgcaactg
     2461 aaggcgcgcg ggcaggcggt tccaccgctc gatgcggtag cggacacgat acgtgaagtc
     2521 ctggtgcagc gtgcgatcag cgatcgcgcc actcagtgga tcgatgaaac ccgcgcagga
     2581 ttgaagatcg acgttatatc cgacgggggc cagccgtgag cgtttctttc aagcggctct
     2641 cggcggcggc gttgatcctg attaccctct tcattttgct ggtggccatc attctgaagt
     2701 cgggatttgc cgagaggtgg atccgtcggg agatcatgag tcgcatcgag cagagcaccg
     2761 gagcgcgcgt cgagattggc gcattccacc tgaatttgtg gcagctcaag ttcgaaatcg
     2821 acgacatcac cgtgcacggg ctcgaaacga cggacatgtc tcctctgttt catgccgacc
     2881 gcgtggatgt aggacttcat attatttcgc ttctgggacg caaggttgcg cttgatgaac
     2941 tgctggtcga acgcccccag gttgctgtct ggatcgatcg ggagggacgc agcaacgtgc
     3001 ctactccgcg cgcgcgatcc acaaatcgtc cgtggcggca gacgttattt aatctgcgca
     3061 tcagacacct ggagctgaag gacggaaccg tttcgtataa caaccggcgc acacctcttg
     3121 ccgtggacgg cggtaatctc gatttcgttt tgcactacga tgctccggcc acgggcgcag
     3181 attcttatgc cggtagtctc gattggcggg acgtgagagt ggcggccaaa cgggatgtgc
     3241 cgttcccgtt cgatttttcg gcgaaattcc tgttgcgtcg cgattctttc gaggccgacg
     3301 aattaatcgt caaggtcttg cactcgcagt tgagccttcg cgccgacctg gccagctttg
     3361 cgcgcgccga ctggaatatc aaatatcgcg gccggcttga tctcgcggac attcggcaca
     3421 tctttaggag ccctcacacg ccgggcggcc atgtcgaatt ctcgggacag gcgcgttacg
     3481 cggcggctgg tttgaatccc aaccgagttc ccggcgccgg atggacctcg agcgggtatt
     3541 acgggagccg ggatatcagc ttgccatata cgtatttcca cgaaagcggc gttcaatccg
     3601 acggcaattt cgaagtggcc aatggacatc ttgttgtacc caatgtgagg gtgcacaacc
     3661 ttggcggtac aacctcggga cggctggaaa tggatttcgc ggggttggta ttccacacca
     3721 aaacgcaaat gcgaggtgtg agcgtcccgc ggctctttga tgcgctggat aatcctaagc
     3781 tgccggttcg atcgctgcac tgggacggcg cgatggaagt cgattcagtg aacacgtggc
     3841 acgccaactt caaggacttt cgcaccacgg gggaaatgag atggtcgcca ccaaaagtgc
     3901 aggtttccgg caaaattccc gcaacggcgc gcgtggaatt cgattatcgc agtgatcggg
     3961 aaacggttgc gatccgccag agtgaaattt ccactcccac ggcgatagta ggtctcgatg
     4021 gagtcctctc cgcagacgac tccgcgctcg aggtgcagtt tcactccagc gatctgactg
     4081 tttgggatga cttcatcagc gcgattcgag ggcccaaggc gggaagccac cgaatcgccg
     4141 gcgaggcaac gtggaccggc cggattctcg gcccattgac gggctcaacg ttcgccgggc
     4201 atcttagctc gcatggcact cagtatgacc agcttacctg ggatgagttg gatggcgata
     4261 tggagtactc gccggacggt tttattttga agaacagcgt gatgcgtcga ggcagcgccg
     4321 cggctaccgt aaatctttcg ctagagttcg atggcgactg gaatttcttg cccactaatt
     4381 cctggatgtt gaacgcccgc atcgaacacg cttccgcaga cgatatgcaa gccattctaa
     4441 aaactggcta ctccgtttcc ggttccctga ccggagaggt gaaaggtagc ggcacccggg
     4501 ccgccccagt gctcgacgct gcgatcatgg ccgaagccat caatgtcgat ggctggagct
     4561 tcaatcattt cgagggccag ctccactggg accatgatga aatgcgcctg tcgcacgcgc
     4621 agctcgtcgg ggatttcgga acgtttgctg gagatatatt ttatcggaca caggaggagg
     4681 aagcggagtt caatgtcagc gggaccggaa tctcacttga taaggtgaaa gaacttcaat
     4741 cggtttcgat gccaattcga gggcaggccg atttcgctct tcggggtaac gggccgctgc
     4801 gaagtccagt tgcacagggc aatttccaca tcaggaattt gacggtggga accgagatgc
     4861 agggagattt tagcggccag ctcgcctcgg acggaaaaac tgctcacctc actttggcat
     4921 cccagcccgc tgagtcgaga ctccacggag acgtttccat cggcctatcg ggagatcagc
     4981 ccgtttcggg gcgtctgtcg atcgagcaat tcgatcttga tccgtttatc agcgcgggtc
     5041 tgcacattca caagctgact ggccacagca ccgccgacgg cgtatttagc atttccggag
     5101 ctttacgccg tccggacaca ttcgaaatgg acgctgacat ttcgcgaatc tcattcacat
     5161 acgagttcgt tcagctgacg aacgacggag atattcggtt ggcctatcgc cgcgatgcga
     5221 tacgggttga gcaggctcat tttcacggcc ctaataccga tttgcagatt ggcggatcgg
     5281 cgcgattcag tcccagcagc cgcctggatt tcaccctgtc gggaggcgtg aatcttcggc
     5341 tgctcggaga agttctgccg gagcttgatg ccgagggacg cgcagacgcc aatatgtccg
     5401 tgcaagggtc catcgctcag ccgattatcg tggggcgggc caagctgcgc gacgcctccg
     5461 ctcactatgc ggattttccg gcaggtttga gccatgttga cggcgatatc gtcttcaaca
     5521 aaagtagcct gagcttcgag caaataaccg ctcaatcggg gggaggccac ctcaccctga
     5581 gtggtaacgt cgcctatggc cagggtccgt tgcactatca ggtgacggtg gcgacgtcca
     5641 cagtccgtat tcgttatccc acaggcatga gttggctggc aggtggaacg gtgcagcttt
     5701 ccggcaccag cagcgccgcg ctgctcagcg ggcgcatcga agtgcagcgc ctgctgtttg
     5761 cccagggtgt cgatatggcc tcgtttttcg cgtcgacgtc cgacacgact cccagggctg
     5821 gctcaccttc cccttttctg cagaacct
//
LOCUS       DATNDT010000508         7723 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_300551, whole genome shotgun sequence.
ACCESSION   DATNDT010000508 DATNDT010000000
VERSION     DATNDT010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7723
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300551"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2163
                     /gene="alaS"
                     /locus_tag="VN788_16555"
     CDS             <1..2163
                     /gene="alaS"
                     /locus_tag="VN788_16555"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HXU22191.1"
                     /translation="EIWNLVFMQYNREAPRDGAPGITLRLLPKPSVDTGAGLERLAAV
                     LQNKISNFDTDLFKPLIDLAANLTGVEVEKERQKLYSLGGALPSRPGGHLPSLRIIAD
                     HSRAATFLVSDGVIPSNDGRGYVLRKIIRRSLGQVRFLKGELPFLSKMSALVRAEMNE
                     AYPELEEHADRITRVLDEEEKRFARTVEVGLRKLDENIRKGFVRDEKLHGIGRASLTL
                     EGGEAFKLYDTYGLPRDFIEDVTRDAGVQVDWAGFDHAMEEQRTRARASWKGTHKEVA
                     NPAYARLADTFKTEPDFYFGTDTRDCRIEAILTKDGPVNEIKKGTEAEIVLDRTSIYS
                     ESGGQVADTGGFYDDSETLEVAEVKGAYYPVTGLIAHRIVAKEDLHVGDRVATVADPE
                     RRARNMRNHTATHLMNAALRNILGTHVKQAGSLVAPDHLRFDFSHFAAVDPSELTDIE
                     QQVNEEIRKNLEITTDIMNIDDALASGALAFFGDKYPEANVRVVTMPDASSPRGFYSK
                     ELCGGTHVHRTGAIGVFKIVGEQSTAAGVRRIEAISGDRALAEYQRTLTTLRTAASLL
                     NASEDQILPVLQRQIDQVRSLEKQLEALKRKAAGSQAQGLLDEIREVKGVRVLAKQVS
                     GFDRESLRNLSDSLRQKIGSGVIVLAAAEDGKVALITAVTRDLTPKLHAGKIVQELAK
                     LVGGSGGGRPDLAEAGGKDTSGIEKALDQVYPLLDRLL"
     gene            2298..3008
                     /locus_tag="VN788_16560"
     CDS             2298..3008
                     /locus_tag="VN788_16560"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HXU22192.1"
                     /translation="MAKGRVTVVILAAGFWFAQPAPSHAQIASYTDGNGKVVYVNKDS
                     PKRRSGSTISSPSGAAAGPASPVLLSPGASSNNWKEDRLDRIVRQAAERHNVDPALVK
                     AVITTESGWNPQAVSRKGAVGLMQLIPETAQRFGVDNSFDPAQNVEGGTSYLRSLLDR
                     YNGDLTKSLAAYNAGERAVDLSGGIPDYRETRMYVRKVTDEYFRPGSGRDPQLWSPPR
                     KPVRQETEANGRIVFTNQ"
     gene            3074..5116
                     /locus_tag="VN788_16565"
     CDS             3074..5116
                     /locus_tag="VN788_16565"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HXU22193.1"
                     /translation="MAIALTLCLALPARADDRSQAGDQFTRAVRMRTMLEGYLEKDRS
                     LSAYQQTVAAYHKVYLISVRANEVPPALVAEAELYEEMGRLYDPKYFQSAIESWDFLL
                     KQYPGSSYRGEALLSIARIQKDDLEQPSDAEATYQDYLKRFPHSQKAKQVREALKNLQ
                     NTELKIADASAKPAPAKDSANATTEAKHVADKDARVNAAYRAADQPPDETGPHADPHI
                     VDEGMATIKALRTWNSQDSTRVVVMLGDTIAFKSAHIASPDRIYFDLSKARLGPKPKI
                     SLDEKAGLLKSVRIAPNRPGVVRLVLDVDGAKDYSAYLLAKPYRLVIDVRARNTENAK
                     GAAAPASASPKNLDAGNEPASGTPTATAEKVSVVSEPAPKAIASDAELPSAKLAAKLV
                     AKTKIVTAKKAAAKEVALLPPAEPKPTRDGQRSLTRALGLKIGRIVIDPGHGGRDTGT
                     IGPHGLMEKDICLDVALRLGKLIEQKLPGAEVIYTRKDDTFISLEERTAIANEAHADL
                     FISIHANSSHDPEARGIETYYLNFATSAESMDVAARENATSDESLHDLQDLIQKIARN
                     EKIEESKELAGDIQDTLSQRLQLVSHRERNRGVKKAPFVVLIGANMPSVLSEISFVSN
                     PSDERLLRKPVQRQRIADGLYRGISSYLENMNSLASNKQKLVSDNHSSDVSSKQDQ"
     gene            5184..6344
                     /locus_tag="VN788_16570"
     CDS             5184..6344
                     /locus_tag="VN788_16570"
                     /inference="COORDINATES: protein motif:HMM:NF042697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6599 family protein"
                     /protein_id="HXU22194.1"
                     /translation="MRCQTWSLAATLLLFAVPAFAQRILPPSVSGWTSQGSPTNQVPT
                     NSAQASAFREYGYVSGEVRSYSHGNDQIAVALYKFKDPSGAYGAYSFLRQPDMAKADL
                     TEHSSISSDRALALTGNLVLDAHAAHIAENERQLKSLVSAAAQQAQDGLLPSLWQHLP
                     QNDIVPRSDRYILGPQTLNQLFPGNLGDSLGFQNGAEAEMAHYRLNGQDVQLLIADFP
                     TPQLAQQQLATLQKKFNVNGSNPAAGSPLYAKRTITLLAVVAGAPSQADAGNLLSAVH
                     SGTELTWNEPTFQFKEPRIEVMIVGSIVGSGIICLFAIIAGISFGGLRLITKRVMPGK
                     VFDRSEYVQILQLGLGSKPINSDDFYGYSPAPTEKVRVDKNLPDRVALRIFR"
     gene            complement(6530..6889)
                     /locus_tag="VN788_16575"
     CDS             complement(6530..6889)
                     /locus_tag="VN788_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007845197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HXU22195.1"
                     /translation="MNHGSEGLFAGMFFMFFLACMVVGYVYVSLALQTIAQKTNTANG
                     WLAWIPIANIILMLEIAKKPIWWIILFLIPLVNLIMSIIVWMGIAEARRKPSWWGILL
                     IVPIVNLIVPGYLAWSD"
     gene            complement(7114..>7723)
                     /locus_tag="VN788_16580"
     CDS             complement(7114..>7723)
                     /locus_tag="VN788_16580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22196.1"
                     /translation="ALLIVVGIPVGGAWAQGWFRHPRDHVLELVLGGLVLFLIFLGLL
                     VLVGVINVMTKDFVVPQMALENVGVMEGWRRLWLWIKSEKGGYGGYIGIKIVLAIGAT
                     IALLIATLIALFALVIIIGIVVVAALLVGKAAGWTWNAYTIALAVVLGCAALAILIFA
                     AALIAVPVIVFFPAYSIYFFAPRYSPLASLLWPEPAAPVAPG"
BASE COUNT         1820 a         2353 c         2087 g         1463 t
ORIGIN      
        1 gaaatctgga atcttgtctt catgcagtac aaccgcgagg ctccaaggga cggcgcccct
       61 gggataacgc ttcggttgct ccctaaacct tcggtggaca ccggcgctgg cctagaacgc
      121 ctcgctgccg tcctccaaaa caaaatcagc aacttcgaca ccgacctgtt caagcctctt
      181 atcgatctcg cggctaacct cacaggcgtt gaggttgaaa aagaacgaca gaaactctac
      241 tcgctggggg gagctctgcc ctcgaggccg ggcggtcatc ttccgtcgct gcgaattatt
      301 gccgaccact ctcgcgccgc gacgtttctg gtctccgacg gcgtcattcc atccaatgac
      361 ggtcgcggct atgtcctgcg caagataatt cgccgctctc tcggccaggt acgctttctc
      421 aagggcgagc ttccctttct ttccaagatg tctgcactcg tccgagccga aatgaacgaa
      481 gcttatcccg aactcgagga gcacgccgat cgcattacgc gcgtacttga cgaagaagaa
      541 aaacggttcg cgcggacggt agaagtgggt ttacgaaaac tcgacgagaa catacgaaaa
      601 ggatttgttc gcgacgaaaa acttcacggg atcggccgcg cttcacttac gctcgaaggc
      661 ggagaggcat tcaaactcta cgacacgtat gggctgccgc gcgacttcat cgaagacgtt
      721 acccgcgacg caggtgttca ggtggattgg gctgggtttg atcacgcgat ggaagagcag
      781 cgcactcgcg ctcgcgcctc gtggaaaggt acgcacaaag aagtcgccaa tcccgcatac
      841 gccaggctcg ccgacacctt caaaaccgag cccgacttct actttggcac cgacacgcgc
      901 gattgccgca tcgaggccat cctcaccaaa gacggcccgg taaacgaaat caaaaaagga
      961 accgaggccg aaatcgtcct cgatcgcacc tccatctact ccgaatcagg cggtcaggtc
     1021 gccgacaccg gcggcttcta cgacgattcc gaaacgctcg aagtcgccga agtaaaaggc
     1081 gcctactatc cggtcaccgg actcatcgcc catcgcatcg tcgccaaaga agatctgcac
     1141 gttggagatc gcgtcgccac cgtcgccgat cccgaacgcc gcgcgcgcaa catgcgcaac
     1201 cacaccgcca cgcacctgat gaacgccgcg ctgcgcaata tcctcggcac gcacgtcaag
     1261 caagccggct cgctcgtcgc gcccgaccat cttcgcttcg atttttccca cttcgccgca
     1321 gtcgatccca gcgagctcac tgatatcgag cagcaggtaa acgaagaaat tcgcaaaaat
     1381 ctcgaaatca ccacggacat catgaatatc gacgacgccc tagcctccgg cgccctcgcc
     1441 ttcttcggtg acaaatatcc tgaagcaaac gtccgcgtcg tcaccatgcc ggacgcgtcc
     1501 tcgccgcgcg gcttctacag caaggagctt tgcggaggca cccacgtaca tcgcaccggc
     1561 gccatcggcg tcttcaaaat cgtaggcgag caatccaccg ccgccggcgt ccgccgaatc
     1621 gaagccatct ccggcgaccg cgccctagcc gagtaccagc gcaccctcac gacactccgt
     1681 accgccgcca gccttttgaa cgcttctgaa gaccaaattc ttccagtact gcagcgtcag
     1741 attgatcaag ttagaagctt ggaaaagcaa ttggaagccc tgaaacgcaa ggctgccggc
     1801 tcccaagcgc aaggattgct cgacgaaatc cgcgaagtga agggtgtccg cgtccttgcc
     1861 aagcaggtca gcggttttga ccgcgaatct cttcgcaatc tttcggattc tcttcgccaa
     1921 aaaatcggct ccggcgtcat cgtcctagcg gccgcagaag acggcaaagt ggctctaatc
     1981 acagcagtta cgagggacct aacccccaaa ctccacgccg gcaagatcgt ccaggagcta
     2041 gctaagctgg tgggcggttc cggcggcggc agacccgatt tagccgaggc tggcggaaaa
     2101 gacacatccg gcatagaaaa agccctcgac caggtttatc ccctcctcga ccggttgcta
     2161 taattcccgt agcgccagaa gcttacgccg ggcagatcct cgtctgcggg gagcttccgg
     2221 cgtcttcaca acactcgaga ttgcgtcgcg agcgagcccc tcgccttccc ctagggccgg
     2281 gagtgagcgt ctagccaatg gccaaaggcc gggtcaccgt tgtaattttg gcggcgggat
     2341 tctggttcgc gcagcctgct ccgtcgcatg cccaaattgc atcctacact gacggcaatg
     2401 gcaaagttgt ctatgtaaac aaggattcgc ctaaaaggcg aagcgggagt actatcagtt
     2461 ctccctcggg ggcggcggcg ggacctgcgt cgcctgtact gctatccccg ggtgcgtcat
     2521 ctaacaactg gaaggaagac cggttggatc gcattgtgcg gcaggcggct gagcgccaca
     2581 acgtggatcc ggccttggta aaggcggtaa tcaccacgga atcggggtgg aatccgcagg
     2641 cggtttcgcg caagggtgcg gtgggattga tgcagctcat tccagagaca gcgcagcgat
     2701 tcggagtcga taattcattc gatccggccc aaaatgtcga gggcggaacg tcgtatctga
     2761 gatcgctgct cgaccggtat aacggcgatt tgacgaaatc gctggccgct tacaatgccg
     2821 gcgagcgcgc cgtggatcta agtggaggca ttcccgatta ccgtgagacg cgaatgtacg
     2881 tccgaaaggt gacggacgaa tatttccggc caggctcagg acgcgatccg cagctgtgga
     2941 gcccgccgcg aaaaccagtg cggcaggaaa cggaagcgaa cggccgcata gttttcacga
     3001 accagtgagg cgaagggtgc ccgtcggatt caaatcggca gccgcaagaa ggcttcccgc
     3061 cgcaggcgcg gccatggcaa ttgccctgac gctctgtctg gccctacccg ctcgcgccga
     3121 tgatcggtcc caggcgggcg accaattcac gcgcgcggtc aggatgcgga cgatgctgga
     3181 gggttacctc gaaaaggatc gctcgctttc cgcctaccag caaactgtcg cggcctacca
     3241 caaggtttac ctgatttccg tgcgcgcgaa cgaagtgccc cctgcgctgg tcgccgaagc
     3301 cgaattgtac gaggaaatgg gccggctcta cgatcccaag tatttccagt ctgcgattga
     3361 atcctgggac ttcctcctga agcagtaccc cggcagcagc tatcgcggag aggctctgct
     3421 atccatcgcg cgcattcaga aggacgactt ggagcagccc agcgacgcgg aagcgacgta
     3481 tcaggattat ttgaaacgct tcccccattc gcaaaaagcc aagcaagttc gtgaggcgct
     3541 gaaaaatctg cagaacacgg aactgaaaat cgccgacgct tccgcgaaac ccgcacctgc
     3601 taaggattca gcaaacgcca cgaccgaagc aaaacacgtg gccgacaaag acgctcgcgt
     3661 caatgccgct tatcgggccg ctgaccagcc gcctgacgag actggaccac acgccgatcc
     3721 gcacatcgtg gacgaaggca tggcaacgat taaggcgctc cgcacatgga actcgcaaga
     3781 ttccacgcgc gtggtggtaa tgctcggcga taccattgcc tttaagtccg cccacattgc
     3841 ctcgcccgat cgcatttatt ttgatctctc caaagcgaga ttaggaccaa agccaaagat
     3901 atccctcgac gaaaaggccg gcttgttgaa atcggtgcgc attgcgccaa ataggccggg
     3961 tgtcgtccgg ctggtgctgg acgtagatgg cgcaaaggat tattcggctt acctgctcgc
     4021 aaaaccttat cggctagtca tcgacgttcg cgcgcggaat acggaaaacg caaaaggcgc
     4081 agcggcccct gcgagtgcgt caccgaaaaa cctcgacgcg ggcaatgaac ctgcatcagg
     4141 cacaccgaca gcgacagccg aaaaagtttc cgtagtttcg gagccggctc caaaagcaat
     4201 tgcatcggat gccgagcttc cctccgcaaa gctggctgcg aaactcgtgg ccaaaaccaa
     4261 aatcgtgacg gcaaagaaag ctgcggccaa agaggttgcg cttctgccgc cggcggaacc
     4321 gaaaccgacg cgcgatggac agcgttccct cactcgcgcg ctcgggttga aaatcggccg
     4381 gatcgtcatc gatccaggac acggcggccg cgacacggga acaatcggtc cccacggctt
     4441 gatggaaaaa gacatttgcc tggacgtggc actgcgctta ggaaagctga tcgaacaaaa
     4501 gctgcctggc gcggaagtga tctacacgcg aaaggacgac accttcattt cgctcgaaga
     4561 gcgaacggcg atcgcgaacg aggcgcatgc cgatctcttc atctcgattc acgccaattc
     4621 gagccatgat ccagaagctc gcggcatcga aacctactat ctgaacttcg ccacctcggc
     4681 cgagtccatg gacgtggccg ctcgcgaaaa tgccacctcc gacgaatcgc ttcacgatct
     4741 ccaagacctg attcagaaga tcgcgcgaaa tgaaaagatc gaggaatcca aggaacttgc
     4801 gggggatatt caggataccc tgagccaaag gcttcaactc gtgagccatc gggaaagaaa
     4861 tcgcggcgtg aaaaaagccc cctttgtagt gctgatcggc gcgaacatgc cgtccgtgct
     4921 ttcagaaatc tcctttgtca gcaatcccag cgatgagcgc ctgcttcgca aaccggtcca
     4981 gcgccagcgg atcgccgacg gcttgtaccg cggaatatct tcctatctgg agaatatgaa
     5041 cagcctcgca tccaataagc aaaaactggt ttccgataac cactcctccg atgtcagctc
     5101 gaagcaggac cagtgacacg gcccgggaat cccaagtaaa atctccggcg tctaatagtg
     5161 gaggaacatt cccgaggata actatgcgtt gccagacttg gtcgcttgct gccactctgc
     5221 tgctattcgc ggttcctgct ttcgcgcaga ggattctgcc accgtccgtt tcaggctgga
     5281 cctctcaagg gtcgccaacg aaccaggtgc caacgaatag cgctcaggcc agcgcgttcc
     5341 gcgaatacgg atacgtctcg ggcgaagtcc gatcctacag ccacggaaac gatcaaatcg
     5401 ccgtagctct ctataagttc aaggatccaa gcggcgctta tggggcctat tcctttttgc
     5461 gccagccaga catggccaaa gcggatttga cggaacattc ctcgatttca tccgatcgcg
     5521 ccctcgcact caccggcaat ttggttctgg acgctcacgc ggctcacatc gcggagaacg
     5581 agcggcaact gaaatccctg gtctccgctg ccgcgcagca agcgcaggat gggcttctgc
     5641 catcgctctg gcagcatctt ccgcagaacg acatcgtccc acgatccgat cgctacatcc
     5701 tcggccctca gacgctcaac cagctctttc ccggcaatct cggcgattcg ctgggctttc
     5761 agaacggcgc cgaggccgaa atggcgcact accgtctgaa tggacaggac gtccaactct
     5821 tgatcgcgga tttccccact cctcaactcg cacaacagca actggctacg ctgcaaaaga
     5881 aattcaacgt gaatggctcg aatccggcgg cgggttcgcc gctatatgca aagcgcacga
     5941 tcactctgct ggccgtcgtc gcaggggcgc ccagccaagc ggatgccgga aatttgctga
     6001 gcgccgttca ttcgggaacg gagctcactt ggaatgagcc gaccttccaa ttcaaggagc
     6061 ctcgaatcga agtgatgatc gtcggatcga tcgtcggatc ggggatcatt tgtctttttg
     6121 cgatcatcgc gggcatctcg ttcgggggtt tgcgacttat aacgaagcgg gtgatgcctg
     6181 gaaaagtgtt cgatcgcagt gaatacgtgc agattttgca actgggtctc ggcagcaaac
     6241 cgatcaattc agacgacttc tacggttaca gtcccgcccc cacggagaag gttcgcgttg
     6301 acaagaatct tcccgacagg gtcgcgctac gcatattccg ctgataatac taggcttagc
     6361 tggacgtatc tacgcctggg tttcgccttg acttcgcaaa acctgcacac taaactgcgt
     6421 ttcaggaacg agttccgtgg cctaccgcgc attggcccag gagctttctc cgttggaggc
     6481 ccgccgcaag gtgggccttc tgtttttttg gccttgtcgg acttacggct caatcagacc
     6541 aagcgagata gcccggcacg ataaggttca cgatcgggac gatgagcaga attccccacc
     6601 agctcggctt gcggcgcgcc tccgcgatcc ccatccacac gattatcgac atgatgaggt
     6661 tcacaagcgg gatcagaaac aggatgatcc accaaatcgg cttcttcgcg atctccagca
     6721 tcaggatgat gttggcgata ggaatccatg ccagccatcc gtttgcagtg ttggtctttt
     6781 gagcgatcgt ttgtagcgcg agtgaaacgt aaacgtaccc caccaccatg cacgcaagaa
     6841 agaacatgaa gaacatccca gcaaacagcc cttcgcttcc gtggttcatg ccagtgcctc
     6901 ctctcgcagc cgatcaggat tagcggaaca gcttcacacc cacttttcaa atcatcacac
     6961 gtgcaggggg cccttttcac aaatcgccat ctcaccatcc aaattggtaa acatcaggcg
     7021 ggaacggcgc gacgctacat ccagtggcgg tgaattgtcc agtaaaatta cgggcaatgt
     7081 tggccggtga tcgggcgtac gttcggtcga cgcctaaccg ggtgcgaccg gggctgccgg
     7141 ttcgggccag agcagcgatg ccagcggcga atatcgcggc gcaaaaaaat aaatggaata
     7201 ggccgggaaa aatacgatta ccggcacagc gatcagcgcc gccgcaaaga taagaatcgc
     7261 cagcgcagca catcccagga ccactgcgag ggcaatggtg taggcattcc aggtccatcc
     7321 cgccgctttt ccgacgagca atgctgcgac cactacgatg ccgatgatga tgactagcgc
     7381 gaagagcgca atcagcgtgg cgatcagcaa ggcaatcgtc gctccgattg ccaacacgat
     7441 tttgattccg atgtatccac cgtagccgcc tttttcggat ttgatccaca accatagcct
     7501 gcgccagcct tccatgacgc caacattttc caaggccatt tgcgggacga caaagtcttt
     7561 ggtcattaca ttgatcacgc ccacaagtac caaaagacca aggaaaatga ggaacaagac
     7621 gagtccgccc agcacgagtt ccagcacgtg atcgcgcgga tgtctgaacc agccctgagc
     7681 ccaggcgccg cctactggaa tgccaaccac aatcaacaac gcc
//
LOCUS       DATNDT010000509         3341 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2167443, whole genome shotgun sequence.
ACCESSION   DATNDT010000509 DATNDT010000000
VERSION     DATNDT010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3341
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2167443"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..809)
                     /locus_tag="VN788_16585"
     CDS             complement(<1..809)
                     /locus_tag="VN788_16585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22197.1"
                     /translation="MGSRSRLSLLLMIVLAVALTNCQQQINFPKPKIEALSPNSIQAG
                     EPSFTLTVTGKSFTPSSSIDWNGQTLASPSNPGLPSTIFVSPTTLTVLIPAILIQNGG
                     VAQVQVSTPQPGGGVTLPLPFTINANASGVPLITSLSPAGAFTGGAGFTLTVTGKNFV
                     SQSIVTVNGNNHPTTFIGSTSLQTSIFAADLGTSGTLQIGVVNPAPNGGSSNSFPLSV
                     KNQTPSLTSLTPASATAGGAATAIGLVGVGFVPNSVVTINGAARTTTFDNST"
     gene            complement(1454..2539)
                     /locus_tag="VN788_16590"
     CDS             complement(1454..2539)
                     /locus_tag="VN788_16590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011404739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HXU22198.1"
                     /translation="MKSNRGRMLRRGLCMAAAVAFVAATGGLLFSQTPGKLSPHGTVE
                     RIKVHGKSLEGNLEGDSPDRYVSVYLPPSYKTHRGQRYPVIYMLHGYTDNDDNWFGAK
                     HPFVNAPEAIDKAIASGSREMIVVMPNAYTVYMGSMYSNSVTTGNWEAFITQDLVSYV
                     DSHYRTIPQRASRGLAGHSMGGYGTIRIGMKYPEVFSSLYALSACCLAPNRNTQGAAM
                     QKAEAIRSAADLAKTDFGTRAMIASAAAWSPDPQKPPLYLDLPVKDGKPQPLIEAKWD
                     ANSPLAMLDQYVPNLKEFHAIALDVGTKDGLMPSIEEMDRDMTTFGIHHTFETYEGTH
                     VSGIQMRLEKKVMPFFSENLSFERAKR"
     gene            complement(2588..2719)
                     /locus_tag="VN788_16595"
     CDS             complement(2588..2719)
                     /locus_tag="VN788_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22199.1"
                     /translation="MPSLILAIGSQDWLTRTVIVGTDKSHANPDYTIFTYCAVLSLG"
     gene            complement(2771..>3341)
                     /locus_tag="VN788_16600"
     CDS             complement(2771..>3341)
                     /locus_tag="VN788_16600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015014030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HXU22200.1"
                     /translation="ARFPWDTGYLSGVGGPFGSFLPSAGPNILASVQPPADLSIDELV
                     ANVQANQNQRLTDTTWTNLTSFSEHGGKLIFYHGTSDPVFSPFDTLAYYEKMAAANGG
                     LDEVRNWSRFYFVPGMNHCTGGPATLDRFDALGAVVDWVEKGTAPDSLIATGKDFPGR
                     SRPLCPYPQHTQYKGQGNPDDASSFECIR"
BASE COUNT          702 a         1024 c          904 g          711 t
ORIGIN      
        1 gtggagttgt caaaagtggt ggtgcgtgcc gctccgttga tcgtcaccac ggaattgggg
       61 acgaaaccca cgccgaccaa cccaatcgcg gtcgccgcgc ctccggcggt cgcggatgcg
      121 ggggtcagcg aagtcaggct gggtgtttgg ttcttcaccg acagcggaaa gctgttggac
      181 gaacccccgt ttggcgctgg attcaccacc ccgatttgca aagttccaga agtaccaaga
      241 tcggccgcaa atatggaagt ttgcagcgaa gttgaaccga tgaaagtagt gggatgattg
      301 tttccgttta cggtaactat cgattgcgag acgaaattct tgccggttac cgtcagcgtg
      361 aatccggcgc ctccggtgaa cgcgcccgca ggcgaaagag aagtaattag cggcacgccg
      421 ctcgcgttgg cgttaatcgt gaaaggaagt ggaagcgtca ccccgccacc cggttgtggc
      481 gtcgacactt gcacctgcgc cacgccccca ttctgaatca ggatggccgg tatcagcaca
      541 gtgagtgtcg tcggactgac gaaaatagtg gacggaaggc ccggattgct cggcgaagca
      601 agcgtctggc cgttccagtc gatggatgat gacggcgtga aggattttcc cgtcacggtc
      661 aaagtaaaac ttggctcgcc tgcctggatg ctatttggag aaagagcctc gatcttcggt
      721 ttaggaaaat tgatctgctg ctggcagttc gtcagcgcca cggccagcac gatcatcagt
      781 aatagtgaaa ggcgagacct actccccaag cttgtcaccc ctgaatcggc aggctgaacc
      841 gccgctaaag caatcgtaga gactactaga gatgacccca accggcaatc aggatgcctc
      901 cgtgcagtag taggggcacc gcatgccgtg cccgccaaac atgcttggct tcctctgccc
      961 acccgcctcg attgcgcgac ccttgcggtt ttcgatgaaa tagggcgcgg ccctgccttt
     1021 tgcggtcgtc ggggcgctcc ttgctgcgcc cgccagccat acttggctcc tcttacgcca
     1081 ttcgtccaag cccatggggc gccgcgcgcc gcaggctcct ctgatcgtca ttccgagcga
     1141 agcgtcccgt gcatttgcct ttgcgcgaag cgccgggacg cgcagtcgag gaatctctct
     1201 tcgatgtaac ctgcggcaaa acgagaaatt ggaacatgct tgtccgccgc tcttgtagcg
     1261 ccgccttgtc ccgagccaag ccgaggggca tcctggcggc gaatttgcct ttcgcacgcc
     1321 ccgacgtaaa aacgcctgcc ggaagccagc gccacaccgc acgcctggaa ctacgtcttg
     1381 cttgatcgat attgttgttc ttctccgcgc cgctcagctc ggctccgtaa tcgtcacgcc
     1441 gcccgcacgc aattcagcgc ttcgcgcgct caaacgataa attctccgag aaaaagggca
     1501 tcaccttttt ctccagccgc atctggattc cgctcacgtg cgtgccttcg tacgtctcaa
     1561 acgtgtgatg aatcccgaaa gtcgtcatat cccgatccat ttcctcaatc gacggcatca
     1621 gcccatcctt cgttcccacg tccagagcga tggcatggaa ttccttcagg ttgggaacgt
     1681 actgatcgag catcgccagt ggcgagttcg cgtcccattt cgcctcaatc aatggctggg
     1741 gcttcccgtc tttcaccgga agatccaggt agagcggtgg cttctgagga tcgggcgacc
     1801 atgccgccgc tgacgcaatc atcgcgcgcg tgccgaaatc cgtcttcgca agatcggccg
     1861 cgctacgaat cgcttcggcc ttttgcatcg ccgcgccttg cgtattccga ttcggagcca
     1921 ggcagcacgc gctcaacgca taaagactcg aaaaaacttc gggatacttc atgccgatgc
     1981 gaatcgttcc atatccgccc atcgaatgcc ctgccagtcc gcggctcgcg cgctgcggaa
     2041 tcgtgcggta gtggctgtca acgtacgaaa ccaaatcttg cgtgatgaac gcttcccaat
     2101 ttcccgtcgt caccgaattc gaatacatgc tccccatgta aaccgtataa gcattgggca
     2161 tcaccacgat catttcgcgc gagccgctgg caatcgcttt atcgatcgcc tcgggcgcgt
     2221 taacaaaagg atgtttggcg ccaaaccagt tgtcgtcgtt gtccgtatac ccgtgcagca
     2281 tgtagatcac gggatagcgc tgcccgcgat gcgttttgta gctgggcggc aaataaacgg
     2341 agacgtagcg atcgggcgaa tcgccttcca aattcccttc gagcgacttg ccatgaacct
     2401 tgatccgttc gacggtaccg tgcggcgaca atttccccgg cgtctgtgaa aaaagtaagc
     2461 cgcccgttgc ggcaacgaac gcaactgcag ccgccatgca caacccgcgc cgaagcatgc
     2521 gcccgcgatt tgatttcatt tgtgctgttc cccccaactt tatccaggcc agcggcctgt
     2581 cagattacta ccccagcgat aggactgcgc aatatgtgaa aatcgtgtag tcagggttcg
     2641 cgtgactctt gtcggtgccc acgatgacgg tgcgcgtaag ccagtcctgc gacccgatcg
     2701 cgagaatcag cgaaggcaaa tcgagtccgg tgtgcgccag gcccgcgtac tcgaagaagc
     2761 tttgtgcgcg ctatctaata cactcgaaac tgctcgcgtc gtctggattg ccttgccctt
     2821 tgtattgcgt gtgctgcgga tacgggcaca agggtcggct acgccccgga aaatctttgc
     2881 cggtagcgat aagcgaatca ggtgccgttc ccttctccac ccaatccact accgccccta
     2941 acgcatcaaa tctatccagc gtagccggtc cgcccgtgca atgattcatc cccggaacaa
     3001 aataaaatcg gctccaattc ctcacttcat cgagcccgcc gttcgccgcc gccatcttct
     3061 cgtaatacgc aagcgtgtcg aacggtgaaa ataccggatc gcttgtgccg tgataaaaga
     3121 tcaacttgcc gccatgctcg gaaaacgacg tgagattcgt ccaagtcgta tccgtcagcc
     3181 gctggttttg attcgcctgt acgtttgcga ccagttcatc gatgctcaaa tcagccggcg
     3241 gctgcaccga agcaagaata ttcggcccgg cgctcggcag aaaactgccg aacggcccgc
     3301 ctacgcctga aagatacccg gtatcccacg ggaaccgcgc g
//
LOCUS       DATNDT010000510         7520 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_157193, whole genome shotgun sequence.
ACCESSION   DATNDT010000510 DATNDT010000000
VERSION     DATNDT010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7520
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157193"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="VN788_16605"
     CDS             complement(<1..603)
                     /locus_tag="VN788_16605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HXU22201.1"
                     /translation="MKSYGTEWIRNVGIVGHGDTGKTQLVSSLLFTAGMTPRPGKVSD
                     GSAVTDWDEEEIARKISINTGLAYAEWLAPESAEKTKINFLDTPGYSTFINETKSSLI
                     AADAALILVDAAAGVQVITEKVWDYAAEYDQPRAFVLNWMDRDLASFERALESLQQVF
                     GRGVVPIQLPIGSDKSFRGVVDLIAMKALTYMPDGDGKAKV"
     gene            complement(1035..2645)
                     /gene="serA"
                     /locus_tag="VN788_16610"
     CDS             complement(1035..2645)
                     /gene="serA"
                     /locus_tag="VN788_16610"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HXU22202.1"
                     /translation="MKIIIADKISERGIALLRETGWEAVISTAASLQSEIANAEGLIV
                     RSATKANAALIEKAPKLRVIGRAGVGVDNVDVEAATRRGILVMNTPGGNAVSVAEHTF
                     ALMLALARPVPQANASIQAGKWEKSAFSGTELRGKTLGLVGLGRVGMEVARRARGLEM
                     KVLAHDPYVTPATAREVEVELVSLEDVLRKSDVVSLHTSLSAATEKMIGAEALAKMKK
                     GARLVNCARGELIDEAALAEALRSGHLGAAALDTFAQEPPKNSPLLGLPNLIATPHIA
                     GSTAEAQEEVGTAIALQVRDYLASGLIRNAVNMPALSPDQYRRIGPYLELGERLGSFI
                     AQAAPSASFTRIRIGYAGEPAELGSHMIRSAVLTGALNSVLDEKVNLVNASSMAAARG
                     LLVEEKTQRRQQGFLNTIEVAIANDGREFTLEGTVGLNGVPRIISLDGMSLEAPLEGT
                     LLLTRNVDVPGVIGRIGTALGSLGVNIATFALGRRAPGGGGEALALVGLDGEISASIV
                     QNIRALPSVTEARLVRLAAAPPLAAAKA"
     gene            2818..4722
                     /locus_tag="VN788_16615"
     CDS             2818..4722
                     /locus_tag="VN788_16615"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HXU22203.1"
                     /translation="MQKTNLIKWSVVLAIAALWTCTASAKHARRVDDKALRDAGQDAG
                     EWLTNGRDYTEQRYSPLKEIDATNVSKLGLAWYYDTGSDRGTVETTPIVSNGVMYATL
                     PWSVVVALDARTGAEKWRWDPKINHMNFPPGSVGKPDKTRIGPSVCCGPANRGVALYN
                     GKVYVGTLDSRLVALDKDTGKIVWEVQTVDKNADYSITGAPRIANGKVIIGNAGGEYA
                     VRGFVTAYDAETGKQIWRFYTVPGDPSKPFENKAMAMAAKTWAGEWWKLGGGGNVWDG
                     ITYDPELNMVYIGIGQGGPWVQGFRDPMTKDNLFLCSIVALNADTGKYVWHFQTTPAD
                     EWDYDAVQQMTLADIKIDGKLRHVIMQAPKNGYFYVLDRKTGKFISGAPYVKVTWSTG
                     LDPKTGRPHEAPNMRYEKHPIEASPGPAGGHAWEAMSYNPNTGLVYLPSLDSTFKYIA
                     AERFKPELGVYNWGIVFRPPPPPPGAKAGELPPPPPNDAFLQARDPVSQKEVWRVTGM
                     NGGGTVTTAGNLVFGASNAGDFVAFSADKGEKLWSVKLLPGIANPVTYSLDGKQYVSV
                     LSGRGGKARIYTFALGANTPIPGRPAGFSDNPFGAPPPAAGARPNAPAAPAQPTAPTT
                     TNPNGTAETH"
     gene            5086..6036
                     /locus_tag="VN788_16620"
     CDS             5086..6036
                     /locus_tag="VN788_16620"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007916144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HXU22204.1"
                     /translation="MKAIRMHSKGGPELLTYEDAPKPQLQPGDALVRVSASSITKTEL
                     TWSETYTACDGTPRLPTIPGHEFSGVVEEITAGVTDVKVGDAVYALAAFCRDGSAAEY
                     VAIRAADLAPKPKTLDHLQAASVPLAALTAWQAFFDHASLTKGQRVLIHGAAGGVGVF
                     AVQIANWAGAEVIATASASNHDFLGELGADQVIDYTELRFEDEVEDVDIVLDAIGGDT
                     LDRSFGVLRRGGTLVSVAGEPSAEKAKHFGVHATYFIVEPNRTQLMEIAHLIDAGKVK
                     PILDTVLPLEQARQAFERGLTNHARGKIVLRIAEQAAARA"
     gene            complement(6502..>7520)
                     /locus_tag="VN788_16625"
     CDS             complement(6502..>7520)
                     /locus_tag="VN788_16625"
                     /inference="COORDINATES: protein motif:HMM:NF013311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HXU22205.1"
                     /translation="AIISTIVEREYVNKEQGRFTPTLLGEKVSDLLVKSFEDIFDVGF
                     TARMEDELDDIEEGKLTWRKSVKEFWDRFEVDLETASDKMESYKAGIPTGKKCEKCGQ
                     GELLERISRHGFFLGCSRYPECDFIQDLAEHGSADVEEGGTVYCDNCGKVMAIKRGRF
                     GTFLACTGYPNCKTTRRLASGTKKALQPDVPIGEKCPEDGGELIRRAGSYGDFIGCQN
                     YPKCKYTRPITLGIKCPKCNEGEFVRRGTAKGRGRGRIFYGCSRYPDCDFTTPFEPIA
                     EPCPKCGAPFIVEKRTKQGNFRACIRENCDWEVAAPEPAPFVHPQEPPAEPQPVGAGD
                     RSRP"
BASE COUNT         1493 a         2529 c         2025 g         1473 t
ORIGIN      
        1 taccttcgcc ttgccgtctc cgtccggcat gtaggtcagc gccttcatcg caatcaaatc
       61 cacaacgccc cgaaaacttt tgtcggagcc gatcggcagt tgaataggca ccacgccgcg
      121 cccaaacacc tgctgcagtg actcgagcgc tcgctcaaaa ctcgccagat cgcgatccat
      181 ccagttcaaa acgaacgcgc gcggctggtc gtattccgcc gcgtagtccc acactttttc
      241 ggtgatcacc tgcacgcccg ccgcggcatc caccagaatc agtgcggcat cggccgcgat
      301 cagcgacgat tttgtctcat taatgaaagt ggaatatccg ggagtatcca gaaaattgat
      361 cttggttttt tccgcggact ccggcgcgag ccattccgca tacgccagac cggtgttgat
      421 cgaaatcttt cgcgcgatct cctcttcgtc ccagtcggtg accgccgaac cgtcgctcac
      481 cttgccgggc cgcggcgtca tcccagccgt gaacagcaac gacgacacaa gctgggtttt
      541 ccctgtgtcg ccgtggccga caatgcccac gttgcgtatc cattcagtgc cataagattt
      601 catgtttcac ctcgtttggc gtacatgtgg tttttgcgga aagctacgaa aaaaatttgc
      661 gaggggcagg catttcgcga gggaaaaatc aggactgcgt tttcgtgctt tgcgctgcgt
      721 tcgatcgaca tcacgaaccc gctccccggc ggctgcgcga taaatcttca agcgcccttg
      781 ggcggggaac gccaagggct ggaaactcgc gatgctagca cacgccagcg cccgccttca
      841 accgcccgtc tagcacgtgg acgccttgct agtgccggtc ccgaagtgtc gtcattctga
      901 gcgtagcgcg ccggtttttc cttccgtgtt cgcgccgtat gcgaacacgc cggtgcgcgt
      961 agtcgaagaa tctctcttcg atgtacgtat cgagcctcta aaagcaagac tcttgcaatc
     1021 ccgacgcagg ctctttatgc cttcgccgcc gcaagcggtg gcgcagccgc cagccgcacc
     1081 agccgcgctt ccgtcaccga cggcagcgcg cgaatattct gcacaatcga cgcgctgatt
     1141 tcgccgtcga gcccaacgag cgccagcgcc tcgcctccgc ctcccggagc gcgccggccc
     1201 agcgcaaacg tggcgatgtt cacacccaga ctccccagcg ctgtcccaat ccgcccgatc
     1261 acgccgggca catccacatt ccgcgtcaga agcagagtgc cttcgagcgg cgcctccagg
     1321 ctcatgccat ccagggaaat aattcggggt acgccattca gtcccaccgt tccttccagc
     1381 gtgaactcgc gcccatcgtt cgcgatggcc acttcgatgg tgttgagaaa gccttgctgg
     1441 cggcgctgcg tcttctcctc gacgagcagt ccccgcgccg cagccatcga agacgcattc
     1501 accaagttga ctttctcatc cagcaccgaa ttcaacgctc cagtcagcac cgcgctgcga
     1561 atcatgtgcg aacccaattc cgccggctct cccgcgtatc cgatgcgaat ccgagtaaag
     1621 ctcgcagacg gcgccgcctg cgcgataaac gatcccagcc gctcgcccaa ttccagatac
     1681 ggcccaattc tccgatattg atcgggtgaa agcgccggca tattcaccgc gttgcgaatc
     1741 aaaccgctgg ctaggtaatc gcgcacttgc agtgcgatcg ccgtgcctac ttcctcctgc
     1801 gcctcggcag tggacccggc gatgtgcggc gtcgcgatca gattcggcaa gccgagcagc
     1861 ggagaatttt tcggcggctc ctgcgcgaac gtatccaacg ccgcggctcc cagatggccc
     1921 gagcgcagcg cctccgccag cgccgcttcg tcaatcaact cgccacgcgc gcaattcacc
     1981 agccgcgcac ccttcttcat tttggccaac gcctccgcgc caatcatctt ttcagtcgcc
     2041 gcgctcagcg atgtgtgcag cgataccaca tccgatttcc gcagcacatc ctccagtgaa
     2101 acgagttcca cttccacttc gcgcgccgtt gccggtgtta cgtatggatc gtgcgccagc
     2161 actttcatct ccagcccgcg cgctcgccgc gccacttcca ttcccacgcg acccagtccc
     2221 accaggccca gagttttccc gcgcaactcc gtccccgaaa acgccgactt ctcccatttc
     2281 cccgcctgta tcgacgcatt cgcttgcggc accgggcgcg ccagcgccag catcaacgcg
     2341 aacgtatgct cggccacgct caccgcgttt cctcccggcg tgttcatcac taaaattccg
     2401 cgccgcgtgg ccgcctccac atccacattg tccacgccca ctcccgcgcg cccgatcacg
     2461 cgcagcttcg gcgctttctc gatcagcgcg gcattggcct tggtcgcgct ccgaacgatc
     2521 aacccctcgg cattcgcaat ttcactttgc agcgacgctg ccgttgaaat caccgcttcc
     2581 cagccggttt cgcgaagcag ggcgatgcct cgttcgctga ttttgtccgc tatgataatt
     2641 ttcatggagc acaaggttat cgggcgcaca ggcaggtgtc aatcgcgcca ccatttacaa
     2701 atttcgttca tcttaagagc atgtgcgcga acctctgcgc tacagcgatt ctagctgtgt
     2761 tcccagcgcc cgatttggtc tagaatgtcc cgcgctataa gccgggggcc atcgaaaatg
     2821 caaaaaacca atttaatcaa gtggtccgtg gtccttgcga ttgcggcgct gtggacatgc
     2881 actgcttccg ccaagcacgc caggcgcgtg gacgacaagg ctctgcgcga tgccggccag
     2941 gatgccggcg agtggctaac caacggccgc gattacaccg aacagcgcta cagcccgctg
     3001 aaagaaattg acgcgaccaa tgtctccaaa ttgggactcg cgtggtatta cgacaccggc
     3061 tccgaccgcg gcacagttga gaccactccc atcgtctcga atggtgtgat gtacgccacg
     3121 ctcccgtgga gcgttgtcgt cgccttggat gcgcgtactg gcgcggaaaa gtggcgttgg
     3181 gatccgaaga tcaaccacat gaatttccct cccgggtcgg tgggtaaacc cgataagact
     3241 cgcatcggcc caagcgtctg ctgcggccct gcaaatcgtg gcgtcgcact ttacaacggc
     3301 aaggtgtacg tcggcactct agactcgcgc ctcgtcgccc tggataagga cactggcaaa
     3361 atcgtttggg aagtccagac agtcgacaaa aacgcggact acagcatcac cggcgctccg
     3421 cgcatcgcca acggaaaagt catcattgga aacgctggcg gcgagtacgc cgtccgcgga
     3481 ttcgtcaccg cctacgacgc cgaaaccggc aagcagatct ggcgcttcta caccgttccc
     3541 ggtgatccct cgaaaccttt tgagaataaa gccatggcca tggccgcgaa gacctgggct
     3601 ggcgagtggt ggaaacttgg cggcggcggc aatgtgtggg acggaatcac ttacgatccc
     3661 gagctaaata tggtttacat cggaatcggc cagggcgggc cgtgggtgca aggctttcgc
     3721 gatcccatga ccaaggacaa tctctttctc tgctccatcg tagctctgaa tgccgacacg
     3781 ggaaaatacg tctggcattt ccagaccacg cccgccgacg aatgggatta cgacgccgtg
     3841 cagcaaatga ctcttgccga catcaaaatc gacggcaaac tccgccacgt catcatgcag
     3901 gcgcccaaaa acggctattt ctacgtgctt gatcgcaaga ctggaaaatt catctccggc
     3961 gcgccctacg tgaaagtcac gtggtccacc ggacttgatc ccaaaaccgg acgtccccac
     4021 gaagccccga acatgcgtta cgaaaagcac cccatcgaag cttcgcccgg ccccgccggc
     4081 ggacacgcgt gggaggcgat gtcttacaac cccaacacgg gactcgttta ccttccctcc
     4141 cttgacagca cgttcaagta cattgccgcg gagcgattca agcccgagct tggcgtttac
     4201 aactggggaa tcgtatttcg gccgccgcct cctccgccgg gcgccaaggc gggagagctc
     4261 ccgcctccgc ctcccaacga cgccttcttg caggctcgcg atccagtcag ccaaaaggaa
     4321 gtctggcgag tcacggggat gaatggtggc ggcacagtca ccactgccgg caatctcgtt
     4381 ttcggagcct ccaacgccgg agacttcgtc gcctttagcg ccgacaaagg tgaaaagctc
     4441 tggtccgtga aactgcttcc gggaatcgcg aaccccgtca cctactcgct ggatggaaag
     4501 caatacgttt cggttctttc cggtcgcggt ggcaaggcta ggatctatac gtttgcgctg
     4561 ggtgcgaaca cgccgattcc aggacggcca gctggcttct ccgataatcc attcggagcg
     4621 ccgcccccag ccgctggtgc acggcccaac gcgcctgcgg ccccggcgca gccaaccgcg
     4681 ccaaccacca ccaatcccaa cggcaccgcc gaaacccact aggcgccttc gcttttgccg
     4741 ttgtacggac atggcatgcc gtgcccggca ataatgcttg gctaccttta cgccccgcct
     4801 ggatcgccag gtgccgaaaa ccgatgtgga gtgcgggagc ttgctcccgc tgtttgccgc
     4861 ccgggcttgc ccgagcgtgc tcctccatcc accaaagaag gcactgctcc tctggaccgt
     4921 cattccgctt gccctgaggc aggaagggag ggaagtgtcc cggccgttgc tcctgcgcga
     4981 agcgccggga cacgcagaga ggaatctctc ttcgatgtac gctagactcg acgtccgcga
     5041 taaaatcctt gtccccgccc caaacgctct ggaaaaggaa aaaccatgaa agccattcgc
     5101 atgcattcca aaggcggacc cgaattgctc acctatgaag acgcccccaa accgcaactt
     5161 cagccgggag acgcactggt ccgagtctcc gcctcatcca tcaccaaaac cgaactaacc
     5221 tggtcggaaa cctacacagc ctgcgatggc acaccgcgcc ttccaaccat ccccggccac
     5281 gaattttcag gcgtggtcga ggaaatcact gccggcgtca cggacgtgaa agtgggcgat
     5341 gcggtctacg cgctcgctgc attctgccgc gacggcagcg ccgcggaata cgtcgccatt
     5401 cgcgccgccg atctcgcgcc aaagccgaag acgctcgatc acctgcaggc cgcttcggtc
     5461 ccgctggccg ccctcaccgc ctggcaagcg ttcttcgatc acgcgtccct caccaagggc
     5521 cagcgcgtgt tgatccatgg cgcggccgga ggcgtgggag tctttgcggt gcaaattgcg
     5581 aactgggctg gcgcggaagt gatcgccacc gcgtctgcaa gcaatcacga tttcctcggc
     5641 gaactcggcg cggatcaggt gatcgactac acggaactgc ggttcgagga cgaagtcgag
     5701 gatgtagata tcgtcctgga cgcgattggc ggggacaccc tcgaccgttc ctttggcgtc
     5761 ctccgccgcg gcggcactct cgtcagtgtt gccggcgagc cgtccgccga gaaagcgaaa
     5821 cacttcggag tgcacgccac gtacttcatc gtcgagccga accgcaccca attaatggaa
     5881 atcgcccact tgatcgacgc tgggaaggtg aagccgatcc tcgacacggt tctgccgcta
     5941 gaacaagcgc gccaagcctt cgaaagaggc ctaaccaatc acgcgcgcgg aaaaatcgtc
     6001 ctgcgcatcg ccgagcaagc cgccgcccgc gcctaagcgg ttggcatcca gcctatttcg
     6061 tcattctgag cggagtgtcc cgcgtctttg cttttgcgcg aagcgccggg acacgcagag
     6121 aggaatctct cttcgatgtg cgccactcgc ctgcgtcgtc agtaggggca cgatacatcg
     6181 tgcccggcaa acatgctcgg ctagatgggc gctatcatcg cgggcgtcat gcacaacgta
     6241 tgtggagtgc ggaggcttgc ctccgctgtc tgccgcccgg ccttgtccgg gcgtgctcca
     6301 acgcgtcagc cacctttccg atgtgcgatt cggcaaacac cgctggctgt catgctgcac
     6361 accggccgcc cagccccgcc gtcattccga gcggagtgtc ccgcgccttt gccttcgcgc
     6421 gaagcgccgg gacacgcaga gaggaatctc tcttcgatgt gggatgggag aaagccctac
     6481 cgctggcggg agaagcaatt cctagggtct tgatcggtcg cctgcgccca caggctgcgg
     6541 ctcagcaggc ggctcctgcg gatggacaaa cggcgcgggc tccggcgcgg ccacttccca
     6601 atcgcaattt tcccggatgc aggcgcggaa attcccctgc ttggtccgct tctcgacgat
     6661 aaacggcgca ccgcacttcg ggcacggctc ggcaataggc tcaaacggag tagtgaaatc
     6721 gcaatcggga taacggctgc agccataaaa aatcctgccg cgccctcgtc ccttcgcggt
     6781 gccgcgtcgg acaaactctc cttcattgca cttcgggcac ttaatcccca gcgtgatcgg
     6841 gcgcgtgtac ttgcacttcg gataattctg gcaaccgatg aaatcgccgt agctcccggc
     6901 ccggcggatt aactccccgc catcttccgg acacttttca ccgatgggga cgtccggctg
     6961 tagtgccttc ttcgtcccgc tcgccagccg ccgcgtggtt ttgcaattcg gataacccgt
     7021 gcacgcgagg aaagtgccaa agcggccgcg cttaatcgcc atcaccttgc cgcaattgtc
     7081 gcaatagacg gtcccgccct cttccacatc cgcgctgccg tgctcagcaa gatcctggat
     7141 gaaatcgcac tccggataac gcgagcaacc gagaaagaat ccgtgccggc tgatgcgttc
     7201 cagcagctcg ccctggccgc acttctcgca ctttttccca gtgggaatcc cggccttgta
     7261 cgattccatt ttgtcggacg cggtttccag atcgacttcg aaccggtccc agaattcctt
     7321 caccgatttg cgccaggtaa gttttccttc ttcgatgtca tccaactcgt cttccatccg
     7381 cgcggtaaag cctacgtcga aaatatcctc gaacgattta accagcagat cgctcacctt
     7441 ttcgcccagc agcgtgggcg tgaagcgtcc ttgctccttg ttcacatact cgcgctccac
     7501 aatcgtcgaa ataatcgcgg
//
LOCUS       DATNDT010000511         3348 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4238588, whole genome shotgun sequence.
ACCESSION   DATNDT010000511 DATNDT010000000
VERSION     DATNDT010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4238588"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="VN788_16630"
     CDS             <1..1295
                     /locus_tag="VN788_16630"
                     /inference="COORDINATES: protein motif:HMM:NF023591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22206.1"
                     /translation="LATTQPEDLSDTIKSRAQLFQFRALTFQEIAGEIERIVKAENLN
                     IETGAVAVIARAAEGSLRDGLSLLEQAIAYSGDAITDAQVRELLGVVAESVLDELVGA
                     VETQSAERALGLVHRLIADGQNLQHFCREAIRHFRNLLVARVCGADSDLVTAPSDERT
                     RLGQQGETFSEEDLTRFFNILLATDDDLRRKPDPRLHLEMGLLRLINAQKLAPLEEVL
                     TEMRSERNVQPHAKHQSSEYARILSATAQPANSSAKSAAASSAAGAGVAPASASASSG
                     SFASVGSARSSSAAPPLPVVPPRIMARAAEEADGLAGARLEAIKAALHESKLLSSFLE
                     QATRCEIEASEFRLFFTTENSALAEMLPREAMERLRTAVHQVIGQPLRVCVKLDSART
                     LTARGSELRARFEEDPVVRAMLEKFGGQISRIKRPGEE"
     gene            1299..1625
                     /locus_tag="VN788_16635"
     CDS             1299..1625
                     /locus_tag="VN788_16635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HXU22207.1"
                     /translation="MEVKALQQMLSQFRQVQETLEKRIGEISVEASAGGGMVFVKMNG
                     QKQLTEVRIEPEIFKSKDQEMLQELILAAVNEATRHVDEELSKQMKDLTGGMSGTMGL
                     KIPGLF"
     gene            complement(1779..2447)
                     /locus_tag="VN788_16640"
     CDS             complement(1779..2447)
                     /locus_tag="VN788_16640"
                     /inference="COORDINATES: protein motif:HMM:NF013340.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HXU22208.1"
                     /translation="MRTRIFLSLLALACLVPLAITPATAQEGPPPNGANGRAGGGGGR
                     PAMGPPLQLTSSAFTDGTMIPDKYTCSPDGKSMTQPANMTSPPLSWVNAPKGTESFVL
                     LLHDPDAHARKSFDDITHWMIFNIPGDATSLPEGVKADSTVGVQAANVTGQPMFFGPC
                     APPGPAHHYTFELFALDTKLDLQKGASRDDVQKAMDGHVLTGTVLIGLFHHEPGFRMG
                     PPPR"
     gene            complement(2704..3156)
                     /locus_tag="VN788_16645"
     CDS             complement(2704..3156)
                     /locus_tag="VN788_16645"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HXU22209.1"
                     /translation="MAPLDSGYPATSDRSSGGTARLGASLHVKGEITGNEDLAIDGSV
                     EGLVQLEDRKLTIGASARVTADVIAREVVVYGNVKGNLRARDRIEIKKDGSVVGDLTT
                     ARIMIEDGAYFKGSIEIDKAGDSSDSSDFDKSSYSKASRTPATASSSD"
BASE COUNT          700 a          943 c         1011 g          694 t
ORIGIN      
        1 ttttggcaac cacacagccg gaggatttat cggacacgat taaatcgcgc gcgcagcttt
       61 tccagtttcg cgcgctcacg ttccaggaaa tcgccggcga aatcgagcgg atcgtgaaag
      121 ccgagaatct gaatattgag acgggtgccg tggcggtgat cgcccgggct gcggaaggca
      181 gcctgcgtga cgggctctct ctgctcgagc aggccatcgc ttactcgggg gacgccatta
      241 cagacgcgca agtacgcgag cttctgggag tggtcgccga gagcgtgctg gacgagttgg
      301 tgggtgcagt tgaaacccag tccgccgaac gtgcgctggg cttggttcac aggctgattg
      361 ccgacgggca aaatctccag cacttttgcc gagaggccat tcgccatttc cgaaacctgc
      421 tggtggcgcg cgtttgcggg gcggactccg atctggtgac cgctccttcg gacgagcgaa
      481 cgagactggg gcagcagggg gagacattca gcgaggagga tctcactcgc tttttcaata
      541 ttctacttgc gaccgacgat gacctgcggc gaaaacccga tccgcggttg catcttgaaa
      601 tgggcctgct cagactcatc aatgctcaaa aattggcgcc gctcgaagaa gtgctaaccg
      661 aaatgcgcag cgagcgaaat gttcagccgc acgcaaaaca ccagtcttcc gaatatgcgc
      721 ggatactttc cgccacggct cagccggcaa attcatccgc aaaatccgcg gcagcttctt
      781 cggcggcagg tgcgggcgtg gctccagcga gtgcttcagc ttcgtcaggc agttttgcga
      841 gcgttggatc agcgcggagt tcgtcggccg ctcctccgct accggtcgta ccacctcgaa
      901 ttatggcgcg tgccgccgaa gaggcggacg gcctggcggg cgcgcggttg gaggcgatca
      961 aggcggcttt gcacgaatcg aaattattgt cgtcgtttct ggagcaggcg acgcgatgcg
     1021 aaatcgaagc gagcgagttc cggctttttt tcacaactga aaacagcgcg ctcgcggaaa
     1081 tgctgccgcg cgaggcgatg gaacgtttgc gcacagccgt gcatcaagtg ataggccagc
     1141 ctctgcgcgt ctgtgttaaa ctggattccg cgcggaccct cacggctcgc ggttcggagt
     1201 tgagagcccg ctttgaagaa gatccggtcg ttagggcgat gctggagaag tttggcggcc
     1261 aaatttccag aatcaaacgc ccgggcgagg aatgaagcat ggaagtgaaa gcattacaac
     1321 agatgctatc gcaattccgg caggtgcaag aaacgctgga aaagcgcatc ggcgaaatct
     1381 ccgtcgaagc ctcggctggc ggcggaatgg tgttcgtgaa aatgaacgga caaaagcagc
     1441 ttacggaagt gcgcatcgag ccggagattt ttaagtccaa agatcaggaa atgctgcagg
     1501 aactcatact ggcggccgtg aatgaagcca cccggcacgt ggacgaagag ctatccaagc
     1561 agatgaagga cctgaccggc ggcatgtctg gaacgatggg cctgaaaatc cccggcctgt
     1621 tctaagaagc cgtcctctgt aactcctcct gcccgtttcc accgtcgtac gttcaatcga
     1681 aaacatattc ctagcaagcg tttgacataa aaaaagaggc gagaatgcgc tcatccctcg
     1741 cgatgaacga tcccgcctct ttcagatcag acaacttctt accgcggtgg aggacccatc
     1801 cggaaaccgg gttcgtggtg gaacaatccg attagcaccg tcccagtgag aacgtgtccg
     1861 tccatggctt tctgcacgtc atcgcggcta gcacctttct gcagatcgag tttcgtatcc
     1921 agcgcgaaaa gctcgaacgt gtagtgatgg gccggtccag gaggcgcgca cgggccaaag
     1981 aacatcggtt ggccggtgac gttggctgct tgtacgccca cggtcgagtc cgctttaacg
     2041 ccttcgggca gagacgttgc gtcccccgga atattaaaaa tcatccagtg cgtgatgtcg
     2101 tcgaacgact tgcgcgcatg ggcgtccgga tcatgcagaa gcagcacgaa actctcagtg
     2161 ccctttggcg cgttgaccca agagagcggc ggagacgtca tgttggccgg ctgcgtcatg
     2221 gattttccat ccggcgagca agtgtacttg tcgggaatca ttgttccatc agtgaatgcg
     2281 ctgctggtta gttgcagagg cggccccatc gccgggcgtc cgccaccgcc gcccgcacgt
     2341 ccgttggcgc cattgggcgg tggtccttct tgcgccgtcg caggagtgat ggcaagcggc
     2401 accaggcatg ccaaagctag aagcgataga aaaattctcg ttcgcattac gtcgttcctc
     2461 cctttccgct gctggaaagt tccagagtcg gttcccccac accttgggtg aagcgtccga
     2521 ttgtaacctc agggggttgg acgcgcaacg aggcgagtgt gtttcaagga gtgtgcacgc
     2581 gccgcttatg gcggtgtaat gcggcgtgcg aaaccgagga agagcgttgt agcgtgatca
     2641 agcgggcgga tgggggcgga ccgctgctgc gcggtccgct caccaaacta ttcagctgta
     2701 agcttaatcc gacgaagagg ccgttgcagg agttcgagac gcctttgagt acgacgactt
     2761 gtcgaaatcg ctgctatcgc ttgaatcgcc ggccttatcg atttcaatcg agcctttgaa
     2821 gtacgcgccg tcttcgatca tgattcgtgc ggtggtcagg tcgccaacca ccgagccgtc
     2881 tttcttgatc tcgatgcggt cgcgcgccct aagattgccc ttcacgttgc cgtacaccac
     2941 tacttcgcgg gcaatcacgt cggcggtaac gcgagcgctc gcgccaatgg tcagctttcg
     3001 atcttcgagc tgaacgagtc cctcgacgct gccgtcgatg gccaggtctt cgttacccgt
     3061 gatttcgccc tttacgtgca aacttgctcc gagccgggcc gtgccaccgc tgctgcggtc
     3121 ggacgtcgcc gggtaacccg aatccaaagg tgccatagtc ttagcctccc cagaaaaaga
     3181 tggtgcaggt ggaacgtacg gcctaggctg ctgcgggagc ggcgtcctag cgggagtttc
     3241 ctgctttttc tctccccaca tttaccctcc ttgggcccga ttgcgccgga caactgatta
     3301 acacagaccg gggtaattca atctattccc gtggcgagcc gacgccaa
//
LOCUS       DATNDT010000512         4105 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2179286, whole genome shotgun sequence.
ACCESSION   DATNDT010000512 DATNDT010000000
VERSION     DATNDT010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4105
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2179286"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VN788_16650"
     CDS             <1..378
                     /locus_tag="VN788_16650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22210.1"
                     /translation="GIAIGAPPPPRVVVVRPARPEPVDVWVDGYWYPVAGRYTWHAGY
                     WTEPAYEGAHWVAPRHEHGRYYEGYWDGPRGRVEHDHHWDHDRDRDFHHDNGRHLGQE
                     KHADHDHHDRDHDDHGHGHGHDK"
     gene            complement(432..893)
                     /locus_tag="VN788_16655"
     CDS             complement(432..893)
                     /locus_tag="VN788_16655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22211.1"
                     /translation="MPGIAIGMRILVVGKPSRGTQAILSRLANMGWGSRTVETLRDAE
                     VLVNTFRFDIVLAAEALPDGTAYEMAGMIARRGGTLLVAIALSESCLWLPTVESGTKV
                     LGTRALNPEQLEQEAEKILRPSDQKQLRTLPPSAHPLPRRAGVPRRKNPAA"
     gene            1555..3054
                     /locus_tag="VN788_16660"
     CDS             1555..3054
                     /locus_tag="VN788_16660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HXU22212.1"
                     /translation="MKSPRANVKTEERLSRSEMLLARAEQLANIGSWELDVASQTMTW
                     SAHFYRMLGLKPGKKPIPHDWGVRSIHPEDRERGLRDMDALISTGVALDNELRFVTPK
                     GAVRLFHSRATVTRNEKGKVVLVRGMSQDITESRAAEIKVRESESLLSYAEEIAHLGS
                     WEFDVARRVSQLSKNLRKMYGLTGNEEFNSDVYDERVHPKDRARVRRILDEAIAMCKP
                     FEYHLRYILPTGETRFLFTRGIPIAGPDGKTVRSFGVVQDITSAKRAQDDLRRLWRQM
                     IQGRDEERRRMARELHESAGQSLAALKMSLSRLYESLPKNKRLAQTLLRSSVELADAA
                     VREVRTVSYLMHPPLLDEAGLGPALRWYARGFAERSKIAVTVDIADDFGRASQDVEMT
                     VFRVVQEALTNVHRYSGSSTACIRVTRENRRLLAEVRDEGCGLAVVGRGPQGPPGVGI
                     AGMRERVQHLNGVFEIESMPGRGTIVRVSLPIDAAADRAFLDESSEKLA"
     gene            3124..3798
                     /locus_tag="VN788_16665"
     CDS             3124..3798
                     /locus_tag="VN788_16665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU22213.1"
                     /translation="MAAKQPYRILIADDHAVVRRGLRALLAAQPGIEVCCEAASGTEA
                     LDYLKKEKPDLMVLDLTMPEMNGLEVARVAREESPTTDILVLSMHYSEEIAREVLRCG
                     AIGYVLKSDADTELLSAVDHARHRQPFFSGKLAVSMAESFKQVPSEAGAPVHKDLEGS
                     PLTNREIQVIQLLADGKSNKQVADTLGVSTRTIESHRNHIMRKMGFTSFSDLIRFAVR
                     TNLIQA"
BASE COUNT          882 a         1143 c         1309 g          771 t
ORIGIN      
        1 ggaatcgcga ttggggcgcc accgccgcct cgcgttgtgg tggtccggcc tgcacggcca
       61 gagcctgttg acgtctgggt ggatggctac tggtaccccg ttgccgggcg ctacacatgg
      121 cacgcgggct attggactga accggcatac gaaggagctc actgggtcgc tccgcgccac
      181 gaacatggac gatactacga gggctactgg gatggtccgc gtgggcgcgt tgaacacgat
      241 caccactggg atcacgatcg agaccgcgat tttcaccacg ataatggccg gcacctcggt
      301 caggaaaaac atgccgacca cgatcatcat gaccgcgacc acgacgatca cggccacggg
      361 cacgggcacg ataagtagcg ggcctcggta aaggcgtcca cgagccgaag gcacaggccg
      421 atggataatc gttaggcggc gggatttttg cggcgcggta cgcctgcgcg gcgcggcaga
      481 ggatgcgcgc tgggcgggag agtgcgcaat tgcttttggt cgctcgggcg cagaattttt
      541 tccgcctctt gttcaagctg ttcagggttg agcgcgcgcg tgcccaacac cttcgtgcca
      601 gattccaccg tcggaagcca caggcagctt tccgaaaggg cgatggccac cagcagcgtg
      661 ccgccgcgcc gcgctatcat ccccgccatc tcataggcgg tgccgtcggg gagcgcttcg
      721 gctgcaagaa cgatatcgaa gcggaaggtg ttcaccagga cttcggcgtc gcgcagggtt
      781 tcaactgtgc gcgagcccca acccatgttc gcgagccggg acagaatggc ctgcgtccct
      841 cgcgacggct tacccactac caaaatgcgc atccctatgg cgatgccggg catgtttgcc
      901 cctcctgatt tcggcggcgc gaaggcggat gcgtgctctc ggcaattttt tccggagcgg
      961 caattcccgc ttccaacgtg tgcgggcgcg atgagtgcaa atcccgttga ccaactcgtc
     1021 gctacgccaa ctcgtccgct agcgcccctt gtaattgctt atcggaaacg atggtatttg
     1081 gcgttagtca aagattgctc gtcgcgaatg gagcgagttg atcactgcag gcagaaactt
     1141 tatctttttg tggaaaagag agaagaggtt gcggattctg cgtacaaatt gcgtagttat
     1201 tgtgccgttt tgaaacagat ttcaccttga cacgggattt tacgggacat tacacttcgc
     1261 ttcacttttt tggaggtgcc tgaccctgca ggcgccgcta ccccggggaa ttttcgaggc
     1321 accgccaaca aagccgtcgg ttaccgtacg ttggaggaac ggttgtcctc gtcgcgcagt
     1381 gatgaagagt cagacctagt tacacccagt atcgcaagcc cacaggatgg gttctttccc
     1441 tttcccgaca cccctaagga tgccaagcgg acagcacgcc tgaattctgc cgctcgagct
     1501 gagagagaag cgaggcggtc aaatgccggc aagaagcgat tgcgcaagta tcgcgtgaaa
     1561 tcaccgcgcg ccaatgtgaa gacggaagag cggctgagcc ggagcgagat gctgctggct
     1621 cgagcggagc aactggcgaa catcgggagc tgggagctcg atgtagcgag ccaaacgatg
     1681 acgtggtccg cgcactttta ccgaatgctg ggcctcaagc cggggaaaaa gcccatccct
     1741 cacgattggg gcgtgcggtc gattcacccg gaagatcgcg agcgcgggct gcgcgacatg
     1801 gacgcgctca tctccacggg cgttgccctc gacaacgaat tgcgattcgt cactcccaag
     1861 ggcgcggtgc ggctttttca ttcgcgcgcc acggtaacgc gcaacgagaa agggaaagtt
     1921 gttctcgttc ggggaatgtc gcaagacatt accgagagcc gggcggcgga aatcaaagtg
     1981 cgcgaaagcg aaagcctgct ttcctatgcc gaggagattg cgcatctggg aagctgggaa
     2041 ttcgacgtgg cgcggcgcgt atcgcaatta tcgaagaatt tgcgcaagat gtacgggctc
     2101 acggggaacg aagagttcaa cagcgacgtg tacgatgaac gcgtccaccc gaaggatcga
     2161 gctcgagtac gccgaatcct cgacgaagca atcgccatgt gcaagccttt cgagtaccac
     2221 ttgcgctaca ttctgccgac gggcgaaact cgattccttt tcacgcgcgg cattccgata
     2281 gcgggaccgg acggcaagac tgttcgttca tttggcgtgg tgcaggacat aaccagcgcg
     2341 aaacgcgcgc aagacgactt gcggcggctc tggcgccaaa tgatccaggg gcgcgatgag
     2401 gaaaggcgcc gcatggcgcg ggaactgcac gagtccgcgg ggcaaagcct ggccgcgctg
     2461 aagatgagtc tttcccgctt gtacgagtca ttgccgaaga acaagaggct cgcgcagacg
     2521 ttgttgcgct cgtcggtgga actcgccgat gcggccgttc gcgaggtgcg gacggtttcc
     2581 tatctgatgc acccgccgct tttggacgaa gcagggctgg gaccggcgct gcgctggtat
     2641 gcacgcggat tcgcggagcg gagcaaaatt gcggtgaccg tggatatcgc cgacgatttc
     2701 gggcgcgcat cgcaggacgt cgaaatgacc gtcttccgcg tggtgcaaga ggctttgacc
     2761 aacgtgcatc ggtattccgg tagcagcacg gcgtgcattc gcgttacgcg agagaacagg
     2821 cggcttctgg ccgaagtgcg agatgagggc tgcgggttgg cggtggtggg cagaggtccg
     2881 caggggccac caggagtagg aatagctgga atgcgtgaac gagtgcaaca cctcaacgga
     2941 gtgttcgaga tcgaaagtat gccaggccga ggcacgatcg ttcgggtgag tctccccatc
     3001 gacgcggccg cagaccgagc ctttttggat gaaagctccg agaaactcgc gtaaccggga
     3061 gtgacggaag gagaaaggct agtctggagc gcagaacgcg aatgcgcaac tggccgctta
     3121 cccatggcag cgaaacagcc gtatcgaata cttatcgcag acgatcatgc ggtcgtccgc
     3181 cgaggcctgc gcgcgctttt ggccgctcag ccaggaatcg aggtgtgctg cgaggccgct
     3241 tccggcacag aggccctgga ttacctcaag aaagaaaagc cggatctgat ggtgctggat
     3301 ctaaccatgc ccgaaatgaa cgggcttgag gtggcgcgag tggctcgcga agagtcgcca
     3361 accacggata ttctggtgct atcgatgcac tattcggagg aaatcgcacg cgaggtattg
     3421 cgctgcggcg ccatcggtta cgtgctcaag tccgatgccg atacggagtt gctctccgca
     3481 gtggaccacg cccggcatcg ccagcctttc ttcagcggca agttggctgt gtcgatggcc
     3541 gaaagcttca aacaggtccc cagcgaagcg ggagcgccgg tacataagga cttggaaggc
     3601 agtccgctga cgaatcgcga gatccaggtg attcaactgc tggcagatgg gaaaagcaac
     3661 aaacaggtgg cggatacgct gggcgtttcc acgcgcacca tcgaaagcca ccgtaaccac
     3721 atcatgcgca aaatgggatt tacgagcttc agcgatttaa tccgcttcgc cgtgcgcacg
     3781 aacctgatac aggcgtagta gccaagcatg cttgccgggc gtagcagtgc tacgccctta
     3841 cgaaaaacat gaaagcggcg cgctgggaat cctgtgccgt gacctgcgaa tgcagaagca
     3901 aacggtgacg cccagcgaaa cctgggcaca ctccgaccaa atgaaggtga agtccggaaa
     3961 atactacttc ttcacggttt tgcggcggcc gcggcggcgg acggggcggt cagcgggtgg
     4021 ctcgccaatc agttcggcgc tccaaatcga ggtgacgaac ttcttcattc cgtggtcgtc
     4081 gaaatcgacg ctggtgcgct cgtta
//
LOCUS       DATNDT010000513         5924 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4188105, whole genome shotgun sequence.
ACCESSION   DATNDT010000513 DATNDT010000000
VERSION     DATNDT010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5924
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4188105"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2244)
                     /locus_tag="VN788_16670"
     CDS             complement(<1..2244)
                     /locus_tag="VN788_16670"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HXU22214.1"
                     /translation="MRGSARYVFGAAMVISLVGLSGNYVGPQVADGQNSNSSSTTANN
                     GKPAEEVTRATLKNGLRVVIVADKLAPVATTVVNYLVGSNETPAGFPGTAHAQEHMMF
                     RGARGLSAGQLANIAAAMGGNFNADTQQTVTQYFFTVPAEDVDVALNIEALRMRGSLD
                     TDELWEKERGAIEQEVAQDLSNPEYVFYTKLLTAMFHGTPYEHDALGSRPSFDKTTGA
                     MLRKFYDTWYAPNNAIMVIVGDVNPQQTLSKIKELFGDIPRKKIPQRPQVNLASVSTQ
                     TLNLDTDLPYGLSVVSFRMPGYDSPDFAAADILADVLNSHRADLYALVPQGKALGTGF
                     ELSSMPHASLAYGLAAYPQGGNGQTLVTEIQKIMADTVNNGVPADLVDASKRHELAEE
                     EFQKNSISGLAMEWSNALAVEGRQSPDDDIRAIEKVTVEDVNRVARKYLTPSNAIEAV
                     LTPQPSGKPVSSKSFGGQESLASSPKGPVELPAWAQDAVTRLTIPDLTIHPTVDTLPN
                     GIKLIVQPESISNSVTVLAHIKNNADLETPPGQEGISSVLDELFPYGTRTLDRIAFQK
                     ALDDIGADESAGADFSLNVLSDHFERGMELLADNELHPALPENAFKVVQRQTEGAVAG
                     QLQSPDYLTSRALDSVLYPKDDPQLREATPATVKSLVMQNVTDYYQHVFRPDLTTIVV
                     IGKVTPEQAKAAVEKYFGGWKASGPPPETDLPTVPANVPAVTAVPDKSRVQVNATLAE
                     TLGLNRFN"
     gene            complement(2471..2617)
                     /locus_tag="VN788_16675"
     CDS             complement(2471..2617)
                     /locus_tag="VN788_16675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010653247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HXU22215.1"
                     /translation="MRLILLIILILLVLGALPTWPYSAHWGYYPSGGLGLLLIILIIV
                     MLAG"
     gene            complement(2754..3767)
                     /locus_tag="VN788_16680"
     CDS             complement(2754..3767)
                     /locus_tag="VN788_16680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010582669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HXU22216.1"
                     /translation="MGQKETDQLNQILQAVALSRALCTVAELGVADHIKAGMPRSAKY
                     LAQATGAHERSLYRLLRFLASHGLFQETKGGEFDHTPLSSPLRSDVEGSFRAAAQLFH
                     HEFRAWDGLDHAVRTGEPGFSAVFGQPLFDHVAAHPELGPLLDAGMTCMHGYETASMI
                     DAYDFSPVHVLADIGGGNGSLIGAVLQRYPKMRGILFDLGHVIARAKDTVKKCGVADR
                     CQVIEGSFFETVPAGADAYLFRHIIHDWTDEQSVQILGLCRKVIPKNGRLLLVECVVP
                     PGNEPSLAKDFDMTMMTFPGGMERTESEFRSLFKQAGFELTSVTPTTTMVSVVEGKPI
                     PAV"
     gene            complement(3799..5001)
                     /locus_tag="VN788_16685"
     CDS             complement(3799..5001)
                     /locus_tag="VN788_16685"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HXU22217.1"
                     /translation="MPISVVMPALEMAQDTGTLVSWRKKEGETVTKGEILLDIETDKA
                     VVEVEALADGVLAGVKAQPGDVIPVGQTIAWILGTGESVPVEEPAGPAPLSSAPRPSS
                     TTSQAPQASEGRGATKRVRISPKARRIAQERGVEIDRVRGSGADGEILVADILAAESA
                     GVSAASPTAAKTSTTAGAQPGAQMLSSIGRLMAERTTQSWTTVPHFFLTREIEASGLN
                     KKRGERLQSIEESHKVRLSHTDLLISAVAGALEKHPRLNASWTGQAIRFNQEINVGVA
                     MAVEDGVVTGVVRNANKIDLAEIAVQRRDLADRARAGRLRPNDINGATFTISNLGMYH
                     VNAFTAIIVPPQAAILAVGAIADRVVAVEGKPAVRPMISMTLSCDHRVADGARAAEFM
                     HTLASALQ"
     gene            complement(5025..>5924)
                     /locus_tag="VN788_16690"
     CDS             complement(5025..>5924)
                     /locus_tag="VN788_16690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HXU22218.1"
                     /translation="PILFALQKQGIRIVDGQQMLSDARVIKTQDEIALLNHSAMMVDA
                     AYDELYRAMKPGMRENEAVGLVSKVLYDLGSEYVEAVNAISGERCNPHPHVFSDRVLR
                     PGDPVYYDILHSYMGYRTCYYRTFSIGSASHAMVDAYKQCREYVDASIELIRPGRTTA
                     QVASVWPKAQEFGFPNEESCFGLQFGHGIGLAIWEKPVISRLVSLDQHYELKPGMVFA
                     LETFWPSSDGWSAARIEEEIVVTETGHEVITRFPAEELLVAGQRYYTVNGPLSTTRET
                     EAAPSMHVREMVEAGAKSEARGD"
BASE COUNT         1191 a         1733 c         1679 g         1321 t
ORIGIN      
        1 attgaatcga ttgagcccca gcgtttccgc gagcgtcgca tttacctgca cacggctctt
       61 gtccggcacc gctgtaactg ccggcacatt tgcgggaacc gtcggcagat cggtttccgg
      121 aggtggcccg gatgctttcc acccgccgaa atatttctcc accgcggcct tggcctgctc
      181 cggggtcacc ttgccgatca ccacgatcgt cgtcagatcc ggccggaata cgtgctggta
      241 ataatccgtc acgttttgca tcacgaggga tttcaccgtt gccggcgtgg cctcgcgtaa
      301 ctgcggatcg tctttcggat acaggactga gtccaaggca cgcgacgtca ggtagtcggg
      361 actctgtaac tgtcctgcca ctgcgccctc ggtctgccgc tgcaccactt tgaaagcatt
      421 ctctggcagc gcgggatgca gctcgttgtc ggccagcaac tccatgcccc gttcgaaatg
      481 gtcgctcaga acgttcaggg aaaaatccgc gccggccgat tcgtccgctc cgatgtcatc
      541 aagcgctttc tgaaatgcaa tccgatctag agtgcgcgtt ccgtatggaa aaagctcgtc
      601 gagaacgctg cttatgcctt cttgccctgg cggcgtttcc aaatcggcat tgtttttgat
      661 gtgggccaac acggtgacag agttgctgat cgattcgggc tgcacgatca gcttgattcc
      721 attcggcagc gtgtccacag tcggatgaat agttagatcc ggaatcgtca gccgcgttac
      781 cgcgtcttgc gcccacgccg gcaattcgac aggccctttc ggcgaggacg ccagggattc
      841 ctgaccgccg aaggatttcg acgatacggg ttttccggag ggctgcggcg ttagcaccgc
      901 ctcgattgcg ttgctcggcg tcaaatattt tcgggccacg cggttcacgt cttccaccgt
      961 aactttttct atggcgcgga tatcgtcgtc cggcgattgc cggccctcca cggccaacgc
     1021 gtttgaccat tccatagcca agccggaaat ggagttcttc tgaaattctt cctcggcgag
     1081 ttcgtggcgt ttggaggcat cgacaagatc ggcgggcacg ccgttgttca ctgtgtcagc
     1141 catgatcttc tgaatttccg ttacgagggt ttgaccattg cccccctggg gatatgccgc
     1201 caacccatac gccaaactcg cgtggggcat gctgctcagt tcgaacccgg tccccagcgc
     1261 ctttccctgt ggcaccaacg cgtacaaatc cgcccggtgg ctgtttagca cgtctgccaa
     1321 aatatccgca gccgcaaaat ccgggctgtc gtagcccggc attctgaacg agacaaccga
     1381 caagccgtag ggtagatcgg tatcgagatt cagcgtctgc gtggatacgc ttgcgaggtt
     1441 cacctgcggc cgctgaggaa ttttcttccg cggaatatcc ccaaaaagct ctttgatctt
     1501 gctaagggtt tgctgcggat tcacatcgcc tacgatcacc atgatcgcgt tgttgggtgc
     1561 ataccacgtg tcgtaaaact tgcgcagcat cgcgccggtg gttttgtcaa acgacgggcg
     1621 agacccgagt gcatcgtgct cgtaaggcgt gccgtgaaac atcgccgtca aaagttttgt
     1681 gtagaacacg tactcgggat tggagaggtc ctgcgccact tcctgctcga tcgcgccccg
     1741 ttccttttcc cacaattcgt ccgtgtcgag cgatccgcgc atgcgcaaag cctcgatgtt
     1801 tagagccacg tccacatctt cggcgggaac cgtgaagaaa tattgcgtca cggtctgttg
     1861 cgtgtcggcg ttgaaattgc cgcccattgc cgcggcaata ttcgccaatt ggccggctga
     1921 aagtcctcga gccccgcgaa acatcatgtg ttcctgcgcg tgcgccgttc ccggaaatcc
     1981 cgccggggtt tcgttcgatc ccactagata attcacaacc gtggtagcca ctggcgcgag
     2041 tttgtccgca acaattacca ctcgcaggcc gttttttaat gttgcacgcg tgacttcttc
     2101 ggccggcttc ccgttattcg cagtcgtcga ggatgagttg gaattctggc cgtcggccac
     2161 ctggggtccg acataattcc cagacaatcc tacaagggaa attaccattg ccgccccaaa
     2221 aacgtatcgc gccgatccac gcatgccgcg cctccgagga ccacaatagc atttcgggtg
     2281 cctagaatcg cgcaactaat tttgacatga cgagcagtac ggatcgtgga acttgctccc
     2341 gtgcatgggg agctacggtg tgctaatgac tcgttccgtg gcgggtgcga gtccaaggct
     2401 tcctagacgg acatctccgc ggccgtcgca actcgtgacg gtgtggggcg ggaattacgt
     2461 ggtgaccgat ttagccagcg agcatcacga tgataagaat tatcagtagc aggcccaatc
     2521 caccactcgg atagtatccc caatgcgcgc tatacggcca agtggggagc gcacccaaga
     2581 cgagcaggat caagatgatc agcaggatta gcctcatacg gctcctcgcg cggcagcttg
     2641 agcttcgcgc tgcctgcagc aatcaactga accttagatt cggccgtagc gcccgaagtc
     2701 acatatttag agtgagcaga atgccgagac tgtaagaacg gggcggctgc gaatcacaca
     2761 gccggtatcg gcttgccttc gaccacgctg accatggtgg tcgtgggcgt gactgacgta
     2821 agctcaaaac cagcctgttt gaatagcgac cgaaattcgg attccgttcg ttccataccg
     2881 ccggggaatg tcatcatcgt catatcgaag tcctttgcga gggatggttc atttccaggc
     2941 ggaactacac attccacgag cagcaacctt ccattcttgg ggattacctt tcggcaaaga
     3001 cccagaatct ggacggactg ttcatccgtc caatcgtgaa tgatgtggcg aaacaaatat
     3061 gcgtcagcgc ccgccggtac ggtttcaaag aaactgcctt cgatcacctg gcacctgtcg
     3121 gcgacaccgc acttcttcac agtatctttt gcgcgcgcaa ttacgtggcc taagtcgaat
     3181 agaattcccc gcattttggg ataacgctgg agcaccgctc cgatcaacga tccattgccg
     3241 ccgccgatat ctgctaaaac gtgaaccggg ctaaaatcgt acgcgtcgat catggacgcg
     3301 gtttcgtagc cgtgcatgca cgtcatcccc gcgtcaagca gcggtccgag ttccggatgc
     3361 gccgcgacat gatcgaacaa aggctgtcca aatacggcgc taaaccctgg ctcgccggtt
     3421 cggaccgcat gatccaaacc atcccaggcg cgaaattcat gatgaaacaa ttgcgctgcc
     3481 gcgcgaaacg atccttcgac atcactgcgc agggggctgg aaagcggcgt gtgatcgaac
     3541 tcgccaccct tcgtctcctg aaacaatccg tggctggcaa gaaagcgtag cagccggtag
     3601 agggatcgtt catgggcgcc ggtggcctgg gccaaatatt tagccgatcg cggcattccc
     3661 gccttaatgt ggtccgcgac gccgagctca gccacggtgc aaagcgcccg gctgagcgct
     3721 acggcttgca agatctgatt cagttggtcg gtttctttct gtcccatcag gtcctcgcct
     3781 catgggttcc aggggagttc attgcagtgc cgaggcgagc gtatgcatga attcggcggc
     3841 gcgggctcca tcggcgacac gatgatcgca ggacagagtc attgagatca tcgggcgcac
     3901 ggcgggtttt ccctcgacgg caactacgcg atccgcaatt gcgccaacgg cgagaatcgc
     3961 agcctgcggc ggaacgatga tggccgtaaa tgcgttaacg tggtacatgc cgagattgct
     4021 gatggtgaat gtcgcgccgt tgatgtcatt cggacgaagg cggccagcgc gagcgcggtc
     4081 ggcaagatcc cgccgctgaa cggcgatttc agcgagatcg attttattcg cgttgcggac
     4141 gacgccggta acaactccgt cttcaacggc catggcgacg ccgacgttta tttcctggtt
     4201 gaaacgaatc gcctgcccgg tccaactggc attgagacgc ggatgctttt cgagcgcgcc
     4261 cgccacggca gaaatcagca aatccgtgtg agagaggcgc actttatgtg attcttcaat
     4321 tgattgtaac cgttcccctc ttttcttatt caggccgctt gcttcgatct cacgagtaag
     4381 gaaaaagtgc ggcacggtcg tccagctctg cgtggtcctc tccgccatca ggcgcccgat
     4441 cgaactcagc atctgcgcgc caggctgagc gccagcggta gtcgaggttt tcgcggctgt
     4501 tggcgacgcc gcgctcactc cagcggactc agcggcgaga atatccgcca cgagaatttc
     4561 gccatcggcg cctgagccgc gcacacggtc aatttccacg ccgcgttcct gcgcaattcg
     4621 gcgcgctttt ggggaaattc tgacgcgctt cgtggcgccg cggccttccg aagcctgagg
     4681 cgcttgagaa gttgttgatg acgggcgcgg agcgctactg aggggcgcgg ggccagcggg
     4741 ttcctccacc ggaacggatt cgcccgttcc gagaatccac gcgatcgtct ggccgactgg
     4801 aattacatcg ccaggctgcg ctttcactcc ggcgagaacg ccgtcggcta gagcttccac
     4861 ttcgacaacg gccttgtccg tttcgatgtc gaggagaatc tcacccttgg tgactgtttc
     4921 gccttccttc ttccgccagg agacaagtgt tcccgtgtcc tgcgccattt ctagtgcggg
     4981 cattacaacg ctgataggca ttttggattc gatggagtaa ctggctagtc gccgcgagct
     5041 tccgatttgg ccccggcttc aaccatctcg cggacgtgca tgctcggcgc cgcttccgtt
     5101 tcgcgcgtag tcgagagcgg accattgact gtgtaatacc gctgacccgc gaccagcagt
     5161 tcttcagctg ggaatcgcgt gatcacctcg tgccctgtct ccgtgaccac gatctcttct
     5221 tcgattcgcg cggcgctcca gccatccgaa gaaggccaga atgtttcgag agcgaagacc
     5281 attccgggct tgagttcata gtgctggtcg agcgaaacca gacggctaat caccggtttt
     5341 tcccagatgg ccagaccgat gccgtgaccg aactgaagcc caaagcagga ttcctcattg
     5401 ggaaatccaa attcctgggc cttcggccaa acggaggcga cttgcgcggt ggtgcgcccc
     5461 gggcggatca gttcaatgga tgcgtcgaca tactcccggc attgcttgta ggcatccacc
     5521 atggcatgcg aggcggagcc aatgctgaaa gttctgtagt agcaggtgcg atatcccatg
     5581 taggaatgca ggatgtcgta gtagacggga tcgcccgggc gcagcactcg atcggagaaa
     5641 acgtgcgggt gcgggttgca gcgctcgccg gaaatcgcgt tgactgcctc gacatattcc
     5701 gaacctaagt cgtaaaggac cttgctcacg aggccaaccg cttcgttttc gcgcataccg
     5761 ggcttcattg cgcggtagag ttcgtcatag gcggcatcga ccatcatcgc tgaatggttc
     5821 agcagggcaa tttcatcttg tgttttgatg acgcgggcat ccgacaacat ttgttggccg
     5881 tcaacgatgc gaatgccttg cttttgaagg gcgaaaagaa ttgg
//
LOCUS       DATNDT010000514         8488 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2182196, whole genome shotgun sequence.
ACCESSION   DATNDT010000514 DATNDT010000000
VERSION     DATNDT010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2182196"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            540..1109
                     /locus_tag="VN788_16695"
     CDS             540..1109
                     /locus_tag="VN788_16695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HXU22219.1"
                     /translation="MRAQAKEADERLLIEAAQKDPARFVDLYEDNFERVYAFVSRRIQ
                     SRADVEDITAEVFHHALVNIAKFEWRGVPFAAWLYRIAANAVADRWQRDSREQGNPAS
                     HPADRSESVDVEKSERAARLFNLVKDLPEDQRRVIEMRFAEEKSIREIAQMLGRTPGA
                     VKQLQFRGIQSLRAQFEKVPGKKLSRSHG"
     gene            1309..1791
                     /locus_tag="VN788_16700"
                     /pseudo
     CDS             1309..1791
                     /locus_tag="VN788_16700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020750015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            1801..2580
                     /locus_tag="VN788_16705"
     CDS             1801..2580
                     /locus_tag="VN788_16705"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU22220.1"
                     /translation="MKFIREGFHSITPYLIISGAARWIDFVKQAFGAEEHFRAKRPGA
                     EDVIMHAEVKIGDSMVELADANPQFPAMPCTLLLRVDDPDDVYNRALEAGATVMQPIA
                     DAPYGSRGGTVLDVAGNRLHISKPTGSGVEIFKGFRSVTPHLYATEPVDLIEFLKRAF
                     GGEETYRAEMPDGSIPHAQVRIGDSIVALAGGHGPYTPMPTTLHLYVPDTDSIYERAL
                     RAGATSIQPPADQPYGDRSAGVTDPFRNRWFIATHIRDVQM"
     gene            complement(2618..3250)
                     /gene="leuD"
                     /locus_tag="VN788_16710"
     CDS             complement(2618..3250)
                     /gene="leuD"
                     /locus_tag="VN788_16710"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019122378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HXU22221.1"
                     /translation="MEPFIKHTGMVAPIDRVNVDTDQMVPKQFLKFLTRQGYGRVLFY
                     DWRYLPGEKPNPDFVLNKPRYKGASVLLARANFGCGSSREHAPWAILDYGFRAILTPS
                     YADIFYNNCFKNGLLPVTLSNEQIDELFKRTEANEGYCVTVDLEQQAVTDDKGLRYPF
                     TIDPFRRDCLLKGLDDIGLTLVHEAEISAFEKKNLRVPVMHEALDVKYHH"
     gene            complement(3255..4679)
                     /gene="leuC"
                     /locus_tag="VN788_16715"
     CDS             complement(3255..4679)
                     /gene="leuC"
                     /locus_tag="VN788_16715"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HXU22222.1"
                     /translation="MAPKTLYEKIWDAHVVHEQAGQPSLIYIDRHLVHEGTSPQAFAG
                     LKVAGRKVRRPELTFAVMDHSVSTKNRTIPVADPDAAAQFDALEKNCKESGINLFDMQ
                     SRNQGIVHVIGPELGITLPGTTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQCLS
                     QFKSKTMKIDVQGKRPRGVTAKDIILAIIGKIGIGGGNGHVVEYTGEAVRSLSMEGRM
                     TMCNMSIEGGARAGMVAPDETTFAYVEGRPFTPQGKEFQDAVEYWRSLATDPGAKYDA
                     VVTLQAAEIAPMVTWGTNPGMVTQVTSRVPDPSSFHDPVDRKAAENALAYMDLKPGTP
                     MQEIQLDRVFIGSCTNSRIEDLRAAAKVVSGKHVAKSLKQALVVPGSRVVKVEAEKEG
                     LDKVFREAGFEWRDAGCSMCIAMNDDVLLSGERSASTSNRNFEGRQGKGSRTHLVSPA
                     MAAAAAIEGHFVDIRKWSGADGAE"
     gene            complement(5354..6400)
                     /locus_tag="VN788_16720"
     CDS             complement(5354..6400)
                     /locus_tag="VN788_16720"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HXU22223.1"
                     /translation="METLTERHRASGARLAESDGCVLPETFSDFEKEYRAAHGTVALF
                     DTNWHVILALTGRDRVKYMHAITSNDIQKLADASGTLALLLTPQGRILAELEIYALPE
                     KLLVLSHASQRERTISTLKKYIIGSQVQIEDLTDKVGTIAIEGPRAAAVIEETCGVKL
                     ADLAEMSIREITLEGSPCYLLRRSHFGQPGAELIATRDALPAFWSKLLTGVSAQGGQP
                     IGMTALNTLRLEAGIPWFPADFNDAMIPHEAALEDTHISFNKGCYTGQEIVERVRSRG
                     HVNRKRVPLQFSTSQPPAPGSKLRAGEAEVGFVTSAAFSPAAGTFIGMGYVRREHFAP
                     GSTIELDGAAVTVK"
     gene            complement(6823..7656)
                     /gene="proC"
                     /locus_tag="VN788_16725"
     CDS             complement(6823..7656)
                     /gene="proC"
                     /locus_tag="VN788_16725"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HXU22224.1"
                     /translation="MAHSNSGMQLAVLGAGKMGGILIEAFVKQRLVPAQNIFATVQHP
                     TRDRKDGASLSVGVTNDNRAAVKNADVVLLCVKPQTVAQVVDEIRDHLNAKTLLISIA
                     ASVPTDYIERRIKGKVPVIRAMPNTPSMVGEGITAIAKGKFAAQKDVDTAQRLFGAVG
                     KTVVADEKNMDAITGLSASGPAFIYIILESLAEGGVKAGLSRELATLLASQTALGAAK
                     MVIETGHHPALLKDTVTTPAGCTIDGILELEEGGLRVTLIKAVVKAAERAKQLLVTDE
                     N"
     gene            7855..>8488
                     /locus_tag="VN788_16730"
     CDS             7855..>8488
                     /locus_tag="VN788_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22225.1"
                     /translation="MVQVISLESVPMVERLNIREGTLRTRRLLTGEPGTPGNFSLQLS
                     STPTYYSPRHRHNFDQVRFQLQGDFDFSTDGVMKPGCVAYFPEGTYYGPQLGPLPSET
                     LVLQFGGASGSGYLSVEQFEQATAELAQHGTFAKGVYTTLKPDGAKINKDAYEAVWER
                     VNGRALVYPRERYLRPVFMDPANFDWIPLADQPGADCKHLGEFSELRTRVA"
BASE COUNT         1701 a         2445 c         2482 g         1860 t
ORIGIN      
        1 acatcttcat gaaagccaag ccgaaagtct ttgtgcccgt aaagggtggc tggggccgcc
       61 gcggcgccac caacgtgatt ttgcggtcgg ctacaaaagc gacgctccga cccgcgcttg
      121 tagcggcttg gcgcaaaacc gcgcccaaac gcctcgccca gcggttcaag gaatagccgc
      181 tcgaaaaata cccatgtcac cgaagggcga cgcacgcgag caagctcgct gttgttcggc
      241 ctctcaaaac ggcagtcgtc attctgaggg aagtgaccgg tgactcgcga acgcctttgg
      301 cgcattcgcc ttctgtggag tgcgggagct tgctcccgct gtctgccgca ggggcttgcc
      361 cgtgcgtgct cctcgcatcc gctcgcgcca ctcgtcgtcc taagcggagt ttcgcggggt
      421 tcctgctttc gcgcggtata ggcgcggcgg attctctaaa ggttcatttc gtcatatcgc
      481 gtaacctttc acattctcgc cggttaatgt tatagagaac gcgttttgcg gagatgcttt
      541 tgagggccca agcgaaggaa gcggacgagc ggcttctcat cgaagcggcg cagaaagatc
      601 ccgcgcgctt cgtggatttg tacgaggaca atttcgagcg cgtctacgcg ttcgtttcgc
      661 ggcggatcca gtctcgcgct gatgtcgagg acattaccgc ggaagtcttc catcacgctt
      721 tggtgaatat tgccaaattc gagtggcgcg gcgtcccttt tgccgcctgg ctttaccgca
      781 tcgcggcgaa tgccgtggcc gaccgctggc agcgtgactc gcgggaacag gggaatcctg
      841 catcgcatcc cgccgatcgc tccgaatcgg tcgatgtgga aaagtcggag cgcgccgcgc
      901 ggcttttcaa tctggtcaag gatttgccgg aagaccagcg gcgcgtgatc gaaatgcgct
      961 tcgctgaaga aaaaagtatt cgcgagatcg cacaaatgct tggacgcacg ccgggagctg
     1021 tgaagcagct tcaatttcgc ggcattcaat ctcttcgcgc acagttcgag aaagttcccg
     1081 gcaagaaatt gagtcggtca catggctaat caaacgcaaa gcgaacaact cgacaaggtg
     1141 atcgagcggc ttctcgatcc tgcaggcgcg ccggtgtcgc ccgccgaagc agcgcacccc
     1201 ttcgctgtaa cgttcgcgcc cgtgatccag gtcatccgcg acttgaagaa tcttccgcgc
     1261 cgagatttca gaacatacct gaaagtagat ttggaaagga gagcgtccat ggccagcacc
     1321 gcaaaggcag cacaggaagt tcgcacaacc gccacgccct atctaagtgt tagaggcgca
     1381 gccgctgcga tcgaatttta caaaaaagcc ttcggcgcaa cggaggtagc acgtttgatg
     1441 cagccggatg gaaggatcgg gcacgccgag ataaacatcg acggcgcaag aattatgctc
     1501 gcggatgagt tcccggaaat tggctttcgg agtcctgaat ctcttggtgg atcgtccgtt
     1561 aacattcatc tcgacgtgcc agatgttgac gctgttgcgc gtcaggcgct tgctgccgga
     1621 gcaaaagtgg tgcgcccggt cgcagaccaa ttctacggtg accgttcggg acaactgagc
     1681 gatccgttcg gctacacctg gactgtttcg acgcacaagg aaacgctgac gcccgaggag
     1741 atgcaacgcc gcatgcacga aatgtcacgc cgagagcgcg gctcctccgc gccggcgccg
     1801 gtgaaattca tccgcgaggg ctttcactcc atcacgccgt atctgattat ttccggtgcg
     1861 gcgcggtgga tcgatttcgt caagcaagcg ttcggtgctg aagaacattt ccgcgcgaag
     1921 cgtccgggcg cggaagacgt catcatgcac gccgaagtta aaatcggcga ttcgatggtc
     1981 gagctggcgg atgcgaatcc acagtttcct gcgatgccat gtactttgct actgcgcgtg
     2041 gacgatcccg atgacgtcta caatcgcgca cttgaagccg gggcaacggt gatgcagccg
     2101 attgcggatg cgccctacgg ctcacgcggt gggaccgttc tagatgtggc cggaaatagg
     2161 ctgcacatct cgaagccaac cggctccggc gtcgaaattt tcaaaggttt tcgcagcgtc
     2221 acgccgcatt tgtatgccac ggagccggtg gatttgatcg agttcctcaa gcgcgcattc
     2281 ggcggagaag aaacgtaccg cgcggaaatg ccggatggtt cgattccaca cgcgcaggtt
     2341 cgcatcggcg attcgatcgt cgcgctagct ggcgggcacg gaccctacac gccaatgcca
     2401 acaacgctgc atctgtatgt gcccgacacc gattcgatat acgagcgagc gctgcgagcc
     2461 ggcgcgacat cgattcagcc gcctgccgat cagccttacg gcgatcgcag cgccggagtt
     2521 acggacccgt tccgcaaccg ctggttcatc gccacgcaca tccgcgacgt gcaaatgtaa
     2581 cctgcagctg caaacgcgaa ctgtcgattc tcaacgacta gtggtggtac ttcacgtcca
     2641 gggcttcgtg catgacgggg actcgaagat tctttttctc gaaggcggaa atttccgctt
     2701 cgtgcaccag cgtcaagcca atatcgtcga gccctttgag caggcaatcg cggcggaaag
     2761 gatcaatggt gaagggatag cgcaagcctt tatcgtcggt gacggcttgt tgttcgagat
     2821 cgaccgtcac gcaatatcct tcgttcgctt cagtgcgctt gaaaagctca tcgatctgct
     2881 cgtttgagag agtgacgggc aagaggccgt tcttgaagca gttgttgtag aaaatatcgg
     2941 cgtagctggg cgtgagaatc gcgcggaagc cgtaatcgag aatcgcccac ggcgcgtgct
     3001 cacggctcga tccgcatccg aaattcgcgc gcgcgagtag aacgctggcg cccttgtatc
     3061 tcggcttgtt caacacaaaa tccggattcg gcttctcgcc cggcagatat cgccaatcgt
     3121 aaaacaaaac gcgcccgtat ccctggcgcg tgaggaattt caaaaactgc ttcggcacca
     3181 tttggtcggt gtcaacattc acgcgatcaa tcggcgcgac cattccagta tgtttgatga
     3241 agggttccat cggattattc ggctccgtcc gccccgctcc atttgcgaat atccacgaag
     3301 tggccttcga tcgcggcggc tgcggccatt gcggggctga ccaggtgtgt gcggctgcct
     3361 ttaccttgac gtccttcgaa gttgcgattc gatgtgctgg cggaacgttc gccgctgagc
     3421 agaacgtcgt cgttcatcgc gatgcacatg ctgcaacccg cgtcgcgcca ctcgaatcct
     3481 gcctcgcgga aaaccttgtc gagtccttct ttttcggctt ccactttgac gacgcgcgac
     3541 ccgggaacaa ccagcgcctg cttcagcgat ttcgcgacgt gttttcccga aactactttt
     3601 gcggcggcgc gcaaatcttc gattcgcgaa ttcgtgcagg aaccgatgaa aacgcgatcg
     3661 agttggatct cttgcatcgg agtaccaggt ttcagatcca tgtaggcaag ggcattctcg
     3721 gcggctttgc ggtcaactgg atcgtggaag gaggaggggt cggggacgcg gctggtgacc
     3781 tgcgtgacca tgccgggatt tgttccccag gtgaccattg gcgcgatctc cgccgcctgc
     3841 agggtgacga cggcgtcgta tttcgcgccc gggtcggtgg cgaggcttcg ccaatactcg
     3901 accgcatcct gaaattcctt gccctgcgga gtgaaggggc gcccttcgac gtatgcgaat
     3961 gtggtttcat ccggcgcgac cattcccgcg cgcgcgccgc cttcgatgga catattgcac
     4021 atggtcatgc ggccttccat cgaaaggctg cgaacggcct cgccggtgta ttccacgaca
     4081 tggccattcc cgccgccgat tccgattttg ccgatgatgg cgagaatgat gtctttcgca
     4141 gtgacgccgc gcggacgctt gccctgcacg tcaattttca tcgtcttgga tttaaattgc
     4201 gagaggcact gcgtggcgag cacatgttcc acttcgctgg tgccgatgcc gaatgccagc
     4261 gcgccgaatg cgccgtgtgt ggcggtgtgg ctatcgccgc agacgatcgt agtacccggc
     4321 agtgtgattc ccaactccgg tccaatcacg tggacgatgc cctggttgcg gctttgcata
     4381 tcgaagaggt tgatgccaga ttctttgcaa tttttctcga gagcatcgaa ctgcgcggcg
     4441 gcatcgggat cggcgactgg aatcgtgcga ttctttgtag agacggagtg gtccattacc
     4501 gcgaaggtca gctccgggcg gcgcactttg cgccctgcga ccttgagtcc cgcgaatgct
     4561 tgcggggacg tgccctcgtg aacaaggtgg cggtcgatat agatcagcga aggctggcca
     4621 gcttgttcgt ggaccacgtg cgcgtcccaa atcttttcgt agagtgtttt aggtgccatg
     4681 gacgccgcta ttctgtccct ttctagacat tgtaacaaga ccgaaacgag tccgccaatt
     4741 gaagccgcaa tcccgtggta gggcttggaa gagaaaagtg tctctctgag aaaagtgtcg
     4801 agtagacatg gccgctatcc tttgttttct agaggttgcg ttgcattcta ctcgaccgta
     4861 gtgtcgataa gaaattggga ttcttcctct ggccgaattc gggggcatcg gccgcttgag
     4921 gacaatcctc gtcggacctg tcggggagcc gcatcgagcg ccgtgcgcgc ttcgcgatct
     4981 tccgttcgaa tgccacggtc acatcgacct cctttcgggc gtaaagtttg cctgactcac
     5041 gctaacacgg agcggattta ttctggtcaa gtagctcctg tggacttttt tttcgggcgt
     5101 caaagcggcg tgcgtggagt gaaagccgaa gaaaaagatc caccttcaca atacgaaaat
     5161 ggcgcacccg cgcaagaaga tgccacccta aagggatggc gctacatgaa cccgctgcga
     5221 agcttttccg tagtagcgac gttccccttc ttggagctcg tgagtcagaa gcgaggggca
     5281 ccgccctcac cagacgagta tgggcactct taaattcaaa cccgcaaacc aagtgcgcaa
     5341 gctgaggtgg aacctacttg acggtgacgg cggcgccgtc gagttcgatg gtgctgcctg
     5401 gggcgaaatg ttcgcggcgg acgtagccca ttccgatgaa tgtgcctgcc gcgggagaga
     5461 atgccgcgct ggtgacgaaa cctacttcag cttcgccggc gcggagttta ctgccgggcg
     5521 cggggggctg cgacgtggaa aattgcagcg ggactcgctt ccgattcacg tggcctcgcg
     5581 agcgtacgcg ttcgacgatt tcttgtcctg tgtagcagcc tttgttgaag ctgatgtgcg
     5641 tgtcttcgag ggcggcttcg tgtggaatca tggcgtcatt gaaatcagcg gggaaccagg
     5701 ggatgccagc ttcgaggcgc agcgtgttga gtgcggtcat gccgattggc tgaccgcctt
     5761 gcgcactcac gccggtaagc aacttgctcc agaaagcggg aagcgcatcg cgcgtagcga
     5821 ttaattcggc gccgggttgg ccgaaatgcg agcggcgcag cagatagcat gggctgccct
     5881 ctagtgtgat ttcgcgaatc gacatttcag ctagatcggc gagcttcacg ccgcacgtct
     5941 cttcgattac tgcagcggcg cgcgggcctt cgatcgcgat ggtgcccacc ttgtcggtga
     6001 ggtcttcgat ctggacttgg gagccaatga tgtacttctt cagggtagag atggtgcgct
     6061 cgcgctggga tgcatgggag agcacgagta gcttttccgg cagggcgtag atttccagtt
     6121 ccgccaggat gcgcccctgc ggagttagta gaagagcgag agttccactc gcgtcggcaa
     6181 gcttctgaat gtcatttgag gtgatcgcgt gcatgtattt cacgcggtcg cggcccgtaa
     6241 gcgcgaggat tacgtgccag ttggtatcga atagcgcaac ggttccgtgg gccgcgcgat
     6301 attccttttc gaaatcagag aaagtttccg gcagcacgca gccgtcagat tcagcgagcc
     6361 gcgctccgct ggcgcgatgc ctttcagtca gtgtttccat aaaaaatggt tcgtcttcga
     6421 gctcatgtta tcagatgcca acggagcagc ggggacgctg ccatttgcta atgcccgtcc
     6481 ggaccataaa tttgactgtt gactccgacc aagcatgttt cttcgtcagg gttgagagcg
     6541 gctaagacgc gctaaagccc gcggcgctac attaggcgtg aggcttgagt gggatccgac
     6601 ggactgcgcc cacatagccg ccacattcaa tcaccggaag ccttgggtac ttcggaaatg
     6661 cgggatcgct ggcagaaagc tcgcaaagaa tgaatttcga gccgcgcgcc gattcaatcc
     6721 gccgcgcaaa ccggcattta gcgcatagcc ctgggccctc aaattccaag acttcgtcac
     6781 cggcattttg cttttccaaa tgcgactcac tcctccatcc ggctaattct cgtcagtgac
     6841 cagcagttgt ttggcgcgtt ccgccgcttt caccactgct ttaatcagcg tcacgcgaag
     6901 tccgccctct tcgagttcaa gaataccgtc gatcgtgcaa cccgccggag tggtaaccgt
     6961 atccttcagc agcgccggat ggtggcccgt ctcaatcacc atcttggccg cgcccaacgc
     7021 tgtctgcgaa gcaaggagcg tcgcgagttc gcgcgaaagc cctgccttca cgccgccttc
     7081 ggcgagcgat tccagaataa tgtaaatgaa cgccggcccg ctcgcggaga ggcccgtgat
     7141 cgcatccata tttttctcgt ccgccaccac cgttttgccc accgcgccaa agagcctttg
     7201 cgccgtgtca acgtcctttt gcgccgcgaa tttccctttc gcgatcgcgg taattccttc
     7261 ccccaccatc gacggcgtgt tcggcatcgc ccgaatcacc ggcactttgc ctttaattcg
     7321 ccgctcgatg tagtcggttg gcacggacgc cgctatcgaa atcagcaatg ttttcgcatt
     7381 caaatgatcg cgaatctcgt ccactacttg cgccaccgtt tgcggcttca cgcacaagag
     7441 gaccacgtcg gcattcttta ccgcagcccg gttgtcattg gtcacgccga cgctgagcga
     7501 cgcgccgtcc ttgcggtcgc gtgtggggtg ctgcaccgtg gcaaaaatat tctgcgccgg
     7561 cacgagtcgc tgcttcacga acgcctctat cagtatgccg cccattttcc ccgcgcccag
     7621 cacggcgagc tgcatgccgc tatttgaatg tgccatttga cctctttcgc gccgctcgtc
     7681 ttggcgggca aatcgcatgg atgatacagg agcaattttg gatacggaag attatagcga
     7741 ttcaacaggc agcgatcatt aaatcacagg agcgagcggg cttggccgtc ttgcgccgtg
     7801 cgggccttga atgtatattc gcgctcgtcg gcaaacttgg gggcagggaa ctccatggtg
     7861 caagtaatca gcctcgaaag cgttcctatg gtcgagcggc tcaatatacg cgaggggaca
     7921 ctgcgaaccc gccgcctgct gaccggagaa cccggcacgc ccggcaattt ctcgctgcag
     7981 ctttcctcca cgcccaccta ctattcccct cggcatcgtc acaatttcga tcaagtgcgc
     8041 tttcagcttc agggcgactt cgatttctcg acagacggcg tcatgaagcc cggctgcgtc
     8101 gcatattttc ccgaaggcac ttactacggg ccgcagttgg gcccgttgcc gagcgaaact
     8161 ctggttcttc aattcggagg agcgagcggc agcggctatc tctccgtcga acaatttgaa
     8221 caggcaacag ccgagctcgc gcagcacggc acatttgcca aaggtgttta caccacgctc
     8281 aagcccgatg gcgcaaaaat aaataaggac gcttacgagg cggtctggga acgcgtaaac
     8341 gggcgggccc tggtttatcc gcgcgagcgc tatctccgtc ctgtgttcat ggacccggca
     8401 aatttcgact ggattccgct ggcggatcag cccggagcgg attgcaaaca tctcggtgaa
     8461 ttttcggagt tgcgcacccg agttgcac
//
LOCUS       DATNDT010000515        15411 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2076650, whole genome shotgun sequence.
ACCESSION   DATNDT010000515 DATNDT010000000
VERSION     DATNDT010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 15411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15411
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2076650"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..199)
                     /locus_tag="VN788_16735"
     CDS             complement(<1..199)
                     /locus_tag="VN788_16735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22226.1"
                     /translation="MKRIALALTLVFVLGAVTARAQNGVRVNWTASSDAGGNPSLTYN
                     VYRAGSCLGQLTKVNSAPLSGT"
     gene            complement(346..1647)
                     /gene="hflX"
                     /locus_tag="VN788_16740"
     CDS             complement(346..1647)
                     /gene="hflX"
                     /locus_tag="VN788_16740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HXU22227.1"
                     /translation="MTKPQSQSVDRRERVLLVGVSRKKNARTGSNSTANHDFLDELRE
                     LAESAGAAVAGTILQLRDSLDPATLVGRGKLEEIGAEANLRGVNLIIVDHDLTPVQLR
                     NMEKGIGCAVIDRTQLILDIFATHAHTREGQLQVELAQLNYLLPRLAGGSDDLSRLGG
                     GIGTRGPGEQKLETDRRRIRDRVAKIRKAIENVRRQRANRRRARQGVPLGMIAFVGYT
                     NAGKSTLFNALTGSKVLTSNRMFATLDPTIRAIQLPSRRRVLLSDTVGFIRDLPPGLI
                     TAFRATLEEVQEAALIVHVTDISNPHHAEHDGQVRKVLEDLGVANQPRLNVLNKIDRL
                     GEEDLTALTEAAGRGSAKILTSALTGSGLPDLLTAIDRALEIDPLVRLKLRMPASDGR
                     NLSLVEARGRVLRSEFADGHFLVEAELPQSAARRLQQFALD"
     gene            2026..2997
                     /locus_tag="VN788_16745"
     CDS             2026..2997
                     /locus_tag="VN788_16745"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HXU22228.1"
                     /translation="MGYGLAVVGLYRVLYYFHIVQFLHELANVSWWWVLLGMIFDVVS
                     YAVQGLRWKLLLTPFGKVRLTYAIRAVYAGLFTNLIFPLRPGEILRSYLLSDSEDIGL
                     GRVLGSVGVERFVDLVIATALLAMVSLVVELPRRFQRVADTLGIATLLVLAIVVLVIL
                     YIEIKLGTTTGPGTARRRLPGKVMAVLTDLHAMGTAPSFYLAVVTSLLMPLCQVLGLW
                     AMMHSYGLHLSFLAAVVVLLVINLGVSLPNAPANFGSYQLFCVVGLSVFQIEKSTATN
                     FSFFAFVALTLPLALVGFPALLRSGLSLRSMREKVGSLSRQALSGPA"
     gene            3201..5774
                     /locus_tag="VN788_16750"
     CDS             3201..5774
                     /locus_tag="VN788_16750"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HXU22229.1"
                     /translation="MAADFNKHLDRARRSLERNRLREAVAEYQLVLNDVPSNQEALQA
                     LADIYTRLNEPSLAAQYYGMQFDRLLDAGDSAKATAIFSRFLRPFPQQPERLMRYAHL
                     LQKQNHPAEALEQFGAAAEQFIEQHRAIDALACYESMAALDPENSSRHVALAELAEQL
                     RHADLAGRSFLRAGQLTQAAGGLDQALEFFGKAHSLLPNDRTGALLFADAKLRKGDAQ
                     GAATLLEPFAPDEKDTAFLALFGESLLRSGHLERAREALEAYYKRNPHNFEKWFQLIG
                     AYARTGEDAKAATLLAEAKDWMRAGRKDSELVAQLDRLSSAYPASLPLAEAVAGIYEE
                     MNRESKYFDALVRLFDLYLVTGRIKEACEALDRLVDIDPYDYRNQERIAKLEGSVDPS
                     FLQNVLSRAAKAATVSSRAGSFSGAASEASAGAVSDEARAQQTLEDLIVQVEIFLQYS
                     LQSKAVERLERIGELFAGEEDKNERLRGLYERANWWPKGAPRKLALPTSPSPPRSPEP
                     SGSPVTLAAPITPAAETRRDLAAIAEINRLMYRQSTPRELLFATAAEIGKYLGVTRCL
                     IAFGAAGEAPQITAEYFAPGNLAAGASRVPSILAMISKVAPDPLGSIELQASGIPGLR
                     DLGFESALAVVLTDKETQAPSGAMLVGDASARKWKPNESFFLQAVGDQLVLSVSHTRL
                     RSLVRSLAVADERTGLLSRSSYIDCLLMESNRARSQGTPLSLIIVQADRGGELLRQHG
                     DAAVEKYVEQLAHALSSSVRQTDIAVKYTAWSLVFVLPNTSVENASALAEKLRQTAAA
                     VQPSWGSQDLTISAIVAEASSRPADDNEDRVTEWINRAETGLDEARRLGGNALVPLAT
                     P"
     gene            5793..6320
                     /gene="ribH"
                     /locus_tag="VN788_16755"
     CDS             5793..6320
                     /gene="ribH"
                     /locus_tag="VN788_16755"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HXU22230.1"
                     /translation="MARQAGREVRGDLNAAGLRFGIVVSQFNAFITDRLLAGALDALQ
                     RAGADEGQIEIVRVPGSFEIPLTAKRLAGKGRFDAIVCIGCVLRGETSHYDYVASETA
                     RGIQLAQLDTGVPMGFCVLTCDTLEQAIDRAGLKGGNKGYEAGLAAIEMARLLRNLPS
                     HSAPPAASKPLPHRR"
     gene            6320..6724
                     /gene="nusB"
                     /locus_tag="VN788_16760"
     CDS             6320..6724
                     /gene="nusB"
                     /locus_tag="VN788_16760"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HXU22231.1"
                     /translation="MSLRRKSREFALQMLFEWDMTHQDPARVEKLFWKSARASDSTRQ
                     FARELFEGAVLEVGALDQMVDRLADNWRLERLAAIDRSILRLAVYELRLGATPPKVVI
                     DEALELAKKFSSAESPAFLNGILDAAFKSLAT"
     gene            complement(6901..8349)
                     /locus_tag="VN788_16765"
     CDS             complement(6901..8349)
                     /locus_tag="VN788_16765"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HXU22232.1"
                     /translation="MTGRHEAATVGGLSSKIKFGTDGWRGVIAEDFTDDNVRIVVHAI
                     ARYVARAEKPGGGILVGYDTRFGSERFARVAAETVAAAGTPVWLSADACPTPALSLLV
                     RGRAAAAGIQITASHNPYRWNGVKLKASYGSSASAAIVAQVERELEKVLHDGVPLLPP
                     RKDLIHSLDIWAPYLETLDRLVDWSRLRSSKFRFLIDPMHGAGRGVLKNLFRRHGIEV
                     DEIRGNRDPLFGGVNPEPIEPHVEELRRGVRAGDYDAGFAFDGDADRIGAIDHDGTFI
                     TPHQIFSILLWHLVGTRKISGGVVKTFSTTKMIDKVSARFGRQLWETPIGFKYICDRM
                     LESDVVLGGEESGGIATKLHLPERDATVSALLLAEVMAWHGKRLGDLVAMLHREFGEH
                     HYGRVDLKVRDGQKEKAIERFSDGKFKKFLDWPIVCREDLDGIKVYLGDVGWAMIRAS
                     GTEPMLRIYAETTRAATTKHVLDEVTTFIRNL"
     gene            complement(8435..9589)
                     /locus_tag="VN788_16770"
     CDS             complement(8435..9589)
                     /locus_tag="VN788_16770"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HXU22233.1"
                     /translation="MTTHARRGAKGDGLPLCAVLLAGGRGTRFWPRSRMHTPKQLLNI
                     VGRETMLRKTMARLSPLIKPRDIWAVTNAEQVAGVRRELRGVPDSQILTEPVGRNTAA
                     AIGLAAIHLMYEHGDALMAVLPADSYVADARRYRALLRAALDLARISGNLAVLGVPPS
                     RSETGYGYIERGGTAGRPRGATAYAVKRFTEKPQLSLARRYVASGKYFWNAGMFFWRV
                     STFLENLRQFLPATHDALVRLGRTIGTARYASTLRQIYPRLENVSVDYAIMEPATRRL
                     GKNTFAHKATRVFVIPAKVGWSDIGSWAAVYELLAPKAGANVFTRRSFALDASGNYFW
                     SPKKFIAAIGVRNLVLVETEDALLLCSRDRSQDVSKIVRWLEEKRLSLLF"
     gene            9763..10311
                     /locus_tag="VN788_16775"
     CDS             9763..10311
                     /locus_tag="VN788_16775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22234.1"
                     /translation="MKLLNWTRRFSRRGFLSGTAATFAAIATTRGAVLTDAQAASRAD
                     SGLRPEEVRTLLKFTRDLFPHDRLDDSFYARVVAPLQQEAAKDASTRRLLSGGIDQLN
                     RLAEASSGKHYVDVSEETARVAIVKKIETGAFFNKVYNDAIVGLYNQPEVWPKFGYEG
                     PSSAKGGYLHRGFSDIDWPGKV"
     gene            10319..11890
                     /locus_tag="VN788_16780"
     CDS             10319..11890
                     /locus_tag="VN788_16780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HXU22235.1"
                     /translation="MPAKFDLNDSSVVVIIGSGAGGGTLANELAQKGISSVVLEAGKR
                     LEIADFKNDEDAMFALFTWLDPRTTSGSWYVAKTSPDLPAWTCKLVGGTTVHWGACAL
                     RMKEYEFKPRTRYGAISGASLLDWPIDLRAMEPYYDRAELKMGVTGTHGIPRLPVSDH
                     GKVLFAGAKKIGYTQFETTNMAINPVPRDGRASCQQTGFCIQGCTFGAKWSTLYTEIP
                     KAEATGKTEIRSESQVLRIEHDASGIVTGVIYADSKGTVQRQKARVVAVAANSIESPR
                     LLLNSSSSKFPQGLANSSGQVGKNYMRHMTSTVFAIFDKPVHMYRGTSQSGIVMDEAR
                     LDTKRGFVGGYQMELLPQGLPSFAQNIVPGAWGRDRASIIDSYSNIAGVWLVGEDLPR
                     ETNRITLHPSRKDKFGMPIPNVHSDDHPNDLAMQKHAIGQADALYKSIGAKRTVHRLP
                     FPSTHNMGTNRMSEKPRDGVVNRYGQAHDIPNLFVSDGSQFTSSSGENPTLTIVALAI
                     RQAEYISDQMSKRTI"
     gene            12013..12504
                     /locus_tag="VN788_16785"
     CDS             12013..12504
                     /locus_tag="VN788_16785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HXU22236.1"
                     /translation="MPYKLTVNGRPATVDVHEDTPLLWVIRDVLNLHGTKYGCGMALC
                     GCCTVHLNGEAVRSCTTPITHAAGKHVTTLEGLSEDGTHPVQLAWKEIDVPQCGYCQA
                     GQMMTASALLAKNPKPTDADIDAAMDGNICRCGTYLRIRKAIHTAAEIAAKRTKKPAA
                     VGL"
     gene            12535..14760
                     /locus_tag="VN788_16790"
     CDS             12535..14760
                     /locus_tag="VN788_16790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HXU22237.1"
                     /translation="METTLVSRRSFLRVTALAGGGMLLASYVEPFGKVLAAQFGPPVT
                     LVPASFIRIAPDGVVTIMAKNPEIGQGVKTMLPMLIAEELDVDWKDVRIEQADLNPKY
                     GLQIAGGSTATPMNWEPLRQVGAGGRQMLIAAAAQKWSVPESECTTTPGRVHHAASKR
                     SAGYGELAATAATLTPPDPKSLKMKDPKDYRIIGKTTPNVDIEKIVTGKPIFGIDFTT
                     PDMLFANFQKCPVYGGKVASANVDELKSEPGVRHAFVIEPVGDSSVLSGGVAVVADTW
                     WQARAALGKLKVTWDEGPTAKQSSKLYATTSVELAQKEPGQVLRKDGDADAAFGNAAH
                     VVEAAYSYPFLSHVPLEPQNCSASFKDGKLEVWAPSQTPANGLGQTAKICGIDPKDIT
                     VHLTRVGGGFGRRLTDDYMVEAAYIAKQVGVPVKLLWTREQDMAHDYYRPAGFHFLKG
                     AVGADGKIVAWRNHFVTFGENDRFAQSAGLSGEEFPSRFVPNFSQLCSMMPTGVPTGA
                     MRAPGSNAIAFVMQSFIDELAHAAGKDPVQFRLDLLATTPLPSAPPPKGTPIAFAPVP
                     FDAPRMKGVLEMVAERSGWGSRKLPGDTAMGVGFHFSHRGYFAEVAEVRVTGGSNVRV
                     NKAWVAADIGSHIINPGAAENLTQGAIIEAMSHAMGYEITIDAGRAVQSNFNNYPPVR
                     LTQAPPEIDVAFLKSDHPPTGLGEPALPPMIPALTNAIFTATGKRVRSLPLQTQGFSW
                     A"
     gene            14934..>15411
                     /locus_tag="VN788_16795"
     CDS             14934..>15411
                     /locus_tag="VN788_16795"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HXU22238.1"
                     /translation="MTSPAYLRIATEEAYAPPELFERYKRLLEDGSHNDPGFASFMGF
                     YLNNSGQRLTEIRAQIQDLGERRIRDMDSTGIAKQILSLTSPGVQIFDAPTAVSLAIA
                     FNDQLAEAIGKHPDRFAGLAAIAPQDPSAAAKELQRAVKSLHLKGAIVNSHTRGEYL"
BASE COUNT         3175 a         4681 c         4298 g         3257 t
ORIGIN      
        1 aggtacctga taggggcgcc gaattcactt tcgtaagttg gccgagacaa gaaccggctc
       61 gatacacatt gtaagtcagt gaagggtttc ctccggcgtc cgaacttgcc gtccagttca
      121 ctcgtacgcc attttgcgct cgggcggtca ctgcacccaa cacgaaaacc agcgttagcg
      181 ctaaagcaat ccgtttcata tgagtcgtcc tttcgtttgc ccgttactgg cgcatcgatc
      241 gacacgctaa tgcgtgcaca cgaaatcgta tcgccgggtc ggcgagtgcg gatttcaggt
      301 ttctgtggac cttttcagag ggaataaaat tcgcgggtgc cgcgcctaat ccagcgcgaa
      361 ctgctggagc cgtctcgcgg ccgactgggg caactccgct tcaaccaaga aatggccgtc
      421 cgcgaactcc gaccgaagca cgcgaccgcg ggcctcaact agcgaaagat ttcgtccgtc
      481 ggatgccggc attcgtagct tgagacgcac gaggggatcg atctccagcg ctcggtcgat
      541 cgctgtgagc aaatccggca gcccggaccc ggtcaaggcc gaagtcagaa tcttcgcgga
      601 cccgcgccca gcggcctcag tcagggccgt caaatcttct tcgcccagcc gatcgatttt
      661 attcaggacg ttcaggcgcg gctggttcgc caccccgagg tcctccagga ccttacggac
      721 ctgtccgtcg tgttccgcat gatgagggtt ggaaatatcc gtgacgtgaa caattaaggc
      781 cgcctcctgc acttcttcaa gtgttgcgcg gaatgcagtg atcagtccgg gaggcaagtc
      841 ccgtataaac ccgacagtat ccgaaagcag aacgcggcgc cgcgaaggta attggatcgc
      901 gcgaatcgtc ggatcgagtg tggcgaacat tctgttcgac gtgagcacct tcgaacccgt
      961 tagcgcatta aacagagtcg attttccagc gtttgtgtat cccacgaagg caatcatgcc
     1021 caaaggcacg ccttgtcgcg cacggcgccg gttggcccgc tggcgacgca cgttttcaat
     1081 tgcctttcga atcttggcca cccggtcccg aattcggcga cgatccgttt ccagcttttg
     1141 ctcgccaggt ccgcgagtac cgatcccacc tcccagacgg gaaaggtcgt cgctgcctcc
     1201 cgccaaccga ggcagcagat aattgagctg cgcaagctcc acttggagtt gtccttcacg
     1261 cgtatgcgcg tgcgtggcga agatgtcgag aataagctgt gtgcggtcta ttacggcgca
     1321 accgatgccc ttctccatat tgcgaagctg caccggcgtc aggtcgtgat ctacaatgat
     1381 gagattgacc ccacgcagat tcgcttccgc tccaatttct tcgagttttc cgcgcccgac
     1441 aagagttgcc ggatcgagcg aatcgcgcag ttgcaaaatc gtccctgcga cggcagcgcc
     1501 tgcgctctcg gccagttcgc gtagttcatc gagaaagtcg tggttggcag tggaattaga
     1561 gccggtccgc gcgttttttt tccgcgacac tcctaccagc aggacccgct cacggcgatc
     1621 aacagattga gattgcggtt tcgtcattcg gacccgggag gtgtggttgg ctcaccggtt
     1681 gatggccaag gctcacggct gtttggatta tacgcctaca gaccctgatt actgacgagg
     1741 tccaatccat ccactccccc ggattgcctg agcgagtccg atgtcgtagg ctgtacccgg
     1801 tctccagcgc ggacaaccgc cccgcgttgg agtctttgag gatgagatga agaaatcacg
     1861 attaatttca cgtggaaccc gtccgcttcc ggaaacaagg gcgcaattgc ctctcgatcc
     1921 cgtcgattcc acctcgtctc aagcgctggg atcgcccatg gaatcgtggc gttgagcgac
     1981 ggctttcagg ctcggaatcc tggaaaggcg agaattcttc gtctcttggg ttacggattg
     2041 gccgtcgttg ggctttaccg ggttctctat tatttccata tcgtgcaatt tctacatgag
     2101 cttgccaacg ttagttggtg gtgggttctc ctcggtatga tcttcgatgt ggtcagctat
     2161 gctgtccaag ggctgcgttg gaaactactc cttacgcctt tcggaaaagt gcggctcacc
     2221 tatgcgattc gagccgtgta tgcgggacta ttcacgaatc tgattttccc gctgcgcccg
     2281 ggtgaaattc tccgcagtta cctgctgtca gattccgaag atattggttt ggggcgcgta
     2341 ctaggttcgg tcggagtcga gcgttttgtg gatttggtga tcgccaccgc gcttctcgca
     2401 atggtctcgc tcgtcgtcga gttaccacgg cgctttcagc gtgtcgcgga cacgctaggc
     2461 atcgcaacac tactcgtgct tgccatcgtg gtgctcgtca ttctctacat tgaaatcaag
     2521 ctcggtacca cgacgggccc cgggactgct cgtcgccgcc tgcccggtaa agtgatggcc
     2581 gtactaacag acctacatgc tatgggcaca gcacccagct tctatttagc cgtggtgacc
     2641 tcgctactga tgcctctgtg tcaggttctc ggtctctggg cgatgatgca ttcttatggt
     2701 cttcatttgt catttctggc cgcggtggtg gtcctgctag tcatcaacct cggcgtttcc
     2761 ctccccaatg cgcccgcgaa cttcggttcg taccagctgt tctgcgttgt cgggctgagc
     2821 gtattccaga tcgagaagtc gacggcaact aacttctctt tctttgcttt cgtcgcactt
     2881 accctcccgc tggctcttgt cggcttcccg gcattgctgc gcagtggcct ttctcttcga
     2941 tctatgcgcg agaaggtggg cagcctgtcc agacaagcct tgagtgggcc agcttaagtg
     3001 ttgtcgtgat cgcttcagcg cacactcgct tgaattcgct tgaggcctaa actggcagcc
     3061 gttatataat cccagtttca gctgcttgcc tttgagcgaa gtcgaaggac gcgcctccga
     3121 aacatcgcgg acacttgtaa ctccccccag agatagaggg gcagttcccg ccgggcaagt
     3181 aaaagagggg ccgctatctc atggctgcgg acttcaacaa acatctcgat cgtgcgcggc
     3241 gcagtctcga gcgcaatagg ttgcgagaag ccgtcgccga atatcagctg gtgctgaatg
     3301 acgtgccttc gaaccaggag gccctccaag ccctggccga catctacact cggctaaacg
     3361 agccgtccct cgcggcacag tactacggaa tgcagttcga tcgcctcctc gatgcgggag
     3421 actccgcaaa ggcgacggct atcttttcgc gctttctgcg cccgttcccg caacagccgg
     3481 aacggctcat gcgttatgcc cacctgctgc aaaagcagaa tcatcctgcg gaagctcttg
     3541 aacagttcgg agctgctgcc gagcagttca tcgagcaaca ccgcgccatt gacgcccttg
     3601 cgtgttatga aagtatggcg gccctcgacc cagagaattc ttcccgccat gtcgcgttag
     3661 cggaactcgc cgagcagctt cggcacgcag acctcgcggg gcgcagcttc cttcgggcgg
     3721 gacaattgac gcaggctgca ggcggccttg accaggcctt ggaattcttt ggaaaggctc
     3781 acagtcttct ccccaatgac cggacgggag cactgttgtt cgctgatgct aagcttcgaa
     3841 aaggtgacgc gcagggtgcc gcgaccctgc ttgagccatt tgctcccgat gaaaaggaca
     3901 ccgcgttctt ggccctcttc ggagaaagtc ttttgcgctc gggccattta gagcgcgccc
     3961 gagaagctct tgaagcttac tacaaacgga atccgcacaa ttttgaaaaa tggttccaac
     4021 tcataggcgc atatgcgcgc acgggcgagg acgcaaaagc ggccaccttg ctagccgagg
     4081 ctaaggactg gatgcgggcc ggccgcaaag atagcgagct cgtcgcccag ttggaccggc
     4141 tgtcttctgc atatcccgca tcactgccgt tggctgaggc ggtggcgggt atttatgagg
     4201 agatgaatcg cgagagcaag tattttgacg ctcttgtccg gctcttcgat ctttatctcg
     4261 tcactgggcg cataaaagag gcctgcgagg cactcgaccg ccttgtcgat attgatccct
     4321 acgattatcg gaaccaagag cgcattgcga agctggaagg tagcgtggac ccctcctttt
     4381 tgcagaatgt tctttctcgc gcggctaagg ccgccactgt atcgtcgcgt gcggggagtt
     4441 tttccggtgc cgcgtccgaa gcctccgctg gcgcagtctc cgacgaggcg cgcgcccagc
     4501 aaacactcga agatttgatc gtgcaggtgg aaatattcct ccaatattcc ctgcagtcca
     4561 aggctgtgga aaggctcgaa cggattggag agctttttgc aggagaggaa gacaagaatg
     4621 agcgattgcg ggggctatac gagcgagcca actggtggcc caaaggagcg ccgcgcaaat
     4681 tggctctgcc aacatcgcca tcgccgcccc ggtctcccga accctcgggg tctcccgtca
     4741 cgcttgcggc accgataacg ccggcagcgg agacccgccg cgacctcgcc gctattgcgg
     4801 aaatcaaccg cctgatgtac cgccagtcaa cgccacgcga actcctattc gcaaccgctg
     4861 ccgagattgg aaaatatctg ggtgtaacac gctgcctcat tgctttcgga gcggccggtg
     4921 aggcgccgca gatcacggct gaatactttg cacccggcaa cctggcagca ggcgcgtctc
     4981 gtgttccttc aatcctcgct atgatttcga aagttgcgcc agatcccctg ggcagcatcg
     5041 aacttcaggc gtcaggcatt ccgggactgc gcgatctcgg gttcgaatcc gccctggcgg
     5101 tggtgctcac ggacaaggaa actcaggctc catccggagc catgctagtc ggggatgcgt
     5161 cggcgcggaa gtggaaacct aacgagagtt ttttcctgca ggcggtaggt gaccagctcg
     5221 tgctctccgt aagccatacg cgactacgct cgttagttcg ttcgctggct gtcgcggatg
     5281 aaaggacggg tctgctcagc aggagttctt atatcgattg tctattgatg gagtcgaatc
     5341 gcgcccgctc gcagggcaca ccactttcgc tcatcatcgt gcaggctgat cgaggcggtg
     5401 agctccttcg tcaacatggt gacgccgccg tcgaaaaata cgttgagcag ctcgctcacg
     5461 cactctcgtc ctctgttcgc cagaccgata tcgccgtcaa atacacagcg tggtcgctag
     5521 tttttgtctt gcctaacacc tcggttgaaa atgccagcgc actcgctgaa aaactccgcc
     5581 aaacggctgc cgcggtgcag ccctcatggg gatcccagga cttgaccatc agtgctattg
     5641 tggcggaagc ttcttcgcgt cctgctgatg acaacgagga tcgggtcacc gaatggatca
     5701 accgcgccga gacaggtctc gacgaagcgc gccggcttgg cggcaacgcg ctcgtgcctc
     5761 ttgcgactcc ctaaattcat ggtgactttc tgatggcgcg acaagccggg cgcgaagtgc
     5821 gtggcgacct gaacgcagcg ggcttgcgct tcgggatcgt cgtaagtcag ttcaatgcct
     5881 tcattacaga ccgattgcta gcgggcgcgc tggatgccct ccagcgtgct ggagcggacg
     5941 aagggcagat tgagatcgtg cgtgtcccag gttcgttcga gataccccta acggccaaaa
     6001 ggctcgcggg aaaaggaaga ttcgatgcca ttgtttgcat cggatgcgta ttgcgcgggg
     6061 agacctccca ttacgattac gtcgcctcgg aaacagcgcg tggcatccag ctcgctcagc
     6121 ttgatacggg cgtacctatg ggattttgtg tgctcacttg cgacacgctc gagcaagcca
     6181 tcgatcgcgc aggcctgaaa ggcggaaaca agggttacga agccggactc gcggcaatcg
     6241 agatggctcg cctcttgcgt aatcttccat ctcattccgc gccacctgct gcttccaagc
     6301 cgctccctca ccggcgctga tgagcctccg ccgcaaatcc cgcgagttcg ccctgcaaat
     6361 gcttttcgaa tgggacatga cgcatcagga ccccgcacgc gtcgaaaaac tcttctggaa
     6421 atcagcgcga gcgtcggatt cgactcgcca gtttgcccgg gaattgtttg aaggagccgt
     6481 actcgaagtt ggcgcccttg accagatggt tgataggctc gcggataact ggaggctcga
     6541 acgcctagcc gccatcgatc gcagcatctt gcggctggcg gtttacgagc tgcgccttgg
     6601 cgcgacgccg ccaaaagtcg tcattgacga ggcgcttgaa ctcgcgaaga agttctcctc
     6661 tgccgaatcg ccggcctttc tgaacggcat cctcgacgct gctttcaaaa gcctcgcaac
     6721 gtaaatcagc gacattcggg tccttacaag attcccttcc acaatcgtgc cgctcgcgac
     6781 ccgggtggca cccatcacgg cagggaagac ttgcaccgcg atcgtcgcgc tgggcagcgc
     6841 cagtcagttt tctaaaaagg cctgttcgct gcaagccggc ctttctaatt cctctttcga
     6901 ttacaggttg cggataaatg tcgtaacttc atcgagcacg tgtttggtag ttgccgcgcg
     6961 agtcgtctct gcgtagattc gcagcattgg ctcagtgccc gaggcccgaa tcattgccca
     7021 gccgacatcg ccgaggtaga cctttatgcc atccagatcc tcgcggcaca cgatcggcca
     7081 atcgagaaac ttcttgaatt tcccatccga aaatcgctca atagcttttt ctttttggcc
     7141 gtctctcacc tttagatcaa cacgtccgta gtgatgctcg ccaaattcgc gatggagcat
     7201 cgcaactaaa tcgccgaggc gcttgccgtg ccaggccata acttctgcaa gtagcaacgc
     7261 gctgacggtg gcgtcgcgct ccggaaggtg cagcttcgtc gcgatcccgc cactttcctc
     7321 tccgcccaac actacgtcgc tttccagcat gcggtcacag atgtatttga aaccgatggg
     7381 cgtctcccaa agctgacggc cgaacctggc ggaaaccttg tcgatcattt ttgtggttga
     7441 gaatgttttc accactccgc cgctaatctt ccgcgtcccc acaaggtgcc acagcagaat
     7501 tgaaaatatc tggtggggcg tgatgaacgt gccatcgtgg tcgatagccc cgatgcggtc
     7561 cgcgtcgcca tcaaacgcga agcccgcgtc atagtcgcca gcgcgcacgc ctcgccgcaa
     7621 ctcctcaacg tgtggctcga tcggctctgg gttcaccccg ccgaaaaggg gatcgcggtt
     7681 gccgcgaatc tcgtccacct cgatgccgtg gcgtcgaaac aagtttttaa gcacgcctcg
     7741 gcctgctcca tgcatcggat caatcaagaa ccgaaactta gaagaacgca gccggctcca
     7801 atccactaaa cgatcgagcg tttcgagata aggcgcccat atatcgagcg aatgaatcag
     7861 gtccttgcga ggcggcaaca gcggcacccc atcatgcaga actttttcga gttcccgctc
     7921 cacttgcgcc acgatggcgg ccgaggcaga gcttccgtag ctcgccttca atttcacgcc
     7981 gttccagcga taaggattgt ggctcgccgt aatctgaatt ccggctgccg cggcgcggcc
     8041 cctcactagc agggaaagcg ccggcgtggg gcacgcatcg gccgaaagcc atacgggagt
     8101 tcccgctgcc gccacggtct cggcggccac ccttgcgaaa cgctcggagc caaagcgcgt
     8161 atcgtatccg accagaatcc cgccaccggg cttttccgcg cgcgccacat agcgtgcgat
     8221 ggcgtgaacg acgatacgca cattatcgtc ggtgaaatct tcggcgatca cgccccgcca
     8281 gccatccgta ccaaatttaa tcttgctgga gagaccgcca acagtggcag cttcgtgacg
     8341 accggtcaac gtcgcccctc gttggttttc tcttcgacta tgcctcatat cgtcgcatca
     8401 agcatatttc cttcgtcgct aaaatcaact ggtttcaaaa gaggagcgac aaccgctttt
     8461 cctccaacca tctcacgatt ttgctgacgt cctgcgaacg gtcgcgggaa cacagcagga
     8521 gtgcgtcctc agtctccacg agaacaagat tgcggacccc aatagcggca atgaatttct
     8581 tggggctcca gaaatagttg ccgctcgcat cgagtgcgaa tgagcgcctc gtgaaaacgt
     8641 tcgcgccggc ctttggcgcc agcaactcat acaccgcggc ccaggaacca atgtcgctcc
     8701 agccgacctt ggctggaatt acgaaaacgc gagtcgcttt gtgtgcaaaa gtatttttcc
     8761 caaggcgacg cgtagcgggt tccatgattg catagtcgac tgagacattt tccagtcgcg
     8821 gataaatttg ccgcagggtc gaggcgtaac gcgccgtgcc gatggtcctt ccaagtctga
     8881 cgagcgcgtc atgcgtggct ggcagaaatt gccgcaaatt ctccaggaac gtggaaaccc
     8941 gccagaagaa catcccggca ttccaaaagt actttcccga tgccacgtac ctccgcgcaa
     9001 gggaaagctg cggcttttcc gtgaatcgct tgaccgcgta ggcagttgcg ccacgcggcc
     9061 ggcccgccgt gcctcctcgt tcgatgtatc cgtatcccgt ctcggaccgg gaaggtggca
     9121 cgccgagtac ggccagattt ccagaaatgc gtgccaaatc cagcgccgcc cgaagaagtg
     9181 cgcgatatcg ccgcgcgtcc gcgacgtagc tatccgctgg cagcaccgcc atcagggcat
     9241 cgccgtgttc atacatcaaa tgaatagcag ccaagccaat cgccgcggcg gtattacggc
     9301 ccacaggctc cgtcaaaatc tgcgaatcag gcacgccacg caactcccgg cgcacaccgg
     9361 ctacttgctc tgcattcgtc acagcccata tatcgcgcgg cttgattaga ggactgaggc
     9421 gggccatcgt tttgcgcagc atcgtctcgc ggcccacgat gttcagcagt tgcttaggcg
     9481 tatgcatgcg gctgcgcggc caaaaacgcg tgccgcgccc cccggcaagc aatacggcgc
     9541 acagcgggag tccgtcgcct ttcgcacccc tcctcgcgtg agttgtcatg cgcgctatag
     9601 tagcggactt tctctagctc gtggttaccg ctttagcgat ttggcgttaa tgtagcaggt
     9661 cgagcggaac cagcaagatt cctcgcgacg agatacttcc aggtaagttg ggatagaatc
     9721 agcagcacca ttcaatagca ggtgaggctg cgggggtgaa caatgaagct gctgaattgg
     9781 acccgaagat tttcccggcg tggttttctc tcgggcaccg ccgccacgtt cgccgccatt
     9841 gctacaaccc gcggcgcggt cctaactgat gcgcaagcgg catcgcgcgc cgactccggc
     9901 ctgcgaccag aagaagttcg cacgctgctg aaattcacgc gcgacctctt cccacacgac
     9961 cgccttgacg attccttcta cgcgagagtg gtcgctcctt tgcagcaaga agctgcaaaa
    10021 gacgcatcca ccaggaggct actctccggc ggcatcgatc aactcaatcg tctcgccgag
    10081 gcttcatcgg gcaaacacta tgtggatgtg tccgaagaaa ctgcgcgcgt ggcgatcgtc
    10141 aagaaaattg aaaccggcgc atttttcaac aaggtctaca acgacgcaat cgtcggactc
    10201 tacaatcaac ccgaagtctg gcccaagttc ggatatgaag ggccttcatc cgcaaagggt
    10261 ggttatctcc accgcggatt cagcgatatc gattggccgg gaaaggtgtg aggatgccat
    10321 gcccgcgaaa ttcgatctga atgattcatc cgtggttgtg atcatcggtt ccggcgccgg
    10381 cggcggcacg ctggcgaacg agctcgcgca aaagggcatc tcatcagtgg tgctcgaagc
    10441 tggcaaacgt ctcgagatcg ccgatttcaa gaatgacgaa gacgccatgt tcgcactctt
    10501 cacttggctc gacccgcgca ctacctcggg ttcttggtac gtcgcgaaaa catcgcccga
    10561 tctgccggcg tggacttgca aactggtcgg tggcaccacc gtgcactggg gcgcctgtgc
    10621 cttgcgcatg aaagaatacg agttcaagcc gcgcactcgt tatggcgcaa tttccggcgc
    10681 cagccttctc gattggccca tcgatctccg cgccatggaa ccgtattacg atcgcgccga
    10741 gttgaaaatg ggcgtcactg gcacgcatgg cattccgcgt ctgcccgtca gtgaccatgg
    10801 caaagtcctt tttgctggcg cgaagaaaat cggctacacc caattcgaga ccaccaacat
    10861 ggccatcaac cctgtacctc gcgatggccg cgcttcctgc cagcaaaccg ggttctgcat
    10921 tcagggatgc actttcggtg cgaagtggtc caccctctac actgaaatcc ccaaggccga
    10981 ggccacgggc aaaaccgaaa ttcgttcgga gtcgcaggtt ctccgcatcg agcacgatgc
    11041 ttcagggata gttaccggtg taatttacgc cgatagcaaa ggcacggtgc agcgccagaa
    11101 ggctcgcgtc gtcgcggtcg ccgcaaattc aatcgaaagc ccgcgtcttc tgctcaattc
    11161 ctcttccagc aagtttccgc aaggcttggc aaactcctcg ggacaagtcg gcaagaatta
    11221 catgcgtcac atgacctcta cggtattcgc gattttcgat aagcccgttc acatgtaccg
    11281 cgggacctcg caatctggaa ttgtgatgga cgaagcgcgc ctggatacta aacgcggttt
    11341 cgttggcggc tatcagatgg agttgttgcc gcagggcctg ccgagcttcg cgcaaaatat
    11401 cgttcccggg gcatggggac gcgatcgcgc ttcgatcatt gacagctact cgaatatcgc
    11461 cggcgtctgg ttagtcggtg aagacttgcc gcgagagacc aatcgaataa cgctgcatcc
    11521 ttcgcgcaaa gacaaattcg gcatgccgat tccaaacgtc cactcggacg atcatcccaa
    11581 cgatctcgcc atgcagaagc acgccatcgg tcaagcggac gctctgtata aatccatcgg
    11641 cgccaaacgg accgttcacc gactgccgtt tccttccacg cacaacatgg gcaccaatcg
    11701 catgtctgaa aagccgcgtg atggcgtcgt gaaccgctac ggccaggcgc acgacattcc
    11761 caacctgttc gtgtccgatg gaagccagtt cacatccagc agcggagaaa atcccacgct
    11821 caccatcgtc gccctcgcca tccgccaggc cgaatatatt tctgatcaaa tgtccaagcg
    11881 gacgatttag gggaaacgct ggacggtaaa gagggtcccc atacatccca tacatgaatt
    11941 gacatcgcgc gcgccccggt gctaacgtgc tgcgcgttag ccaaaagacc tcgtctcccc
    12001 ggaggtgact ccatgcccta caaattaacc gtaaatggac gccctgctac cgtcgatgtg
    12061 catgaggaca ctcccttgct atgggtgatt cgtgacgtcc tcaatcttca tggcacgaag
    12121 tacggctgtg gaatggcgct ctgcggctgc tgcaccgtac acctgaatgg tgaggccgtg
    12181 cgctcctgta ccacgccgat cacccatgcg gccggcaagc acgtcaccac gctcgaaggt
    12241 ctttccgaag acggcactca tcccgtgcag ctcgcgtgga aggaaattga cgtgccgcag
    12301 tgcggctatt gccaagctgg tcaaatgatg accgcctccg ccctgctcgc gaagaatccc
    12361 aaacccacgg acgcggatat agacgctgcc atggacggaa acatttgccg ctgcggcacc
    12421 tatctgcgca ttcgcaaggc aatccatacg gcggccgaaa tcgccgcaaa acggaccaag
    12481 aagccagccg ctgtcgggct ctgaagcgcc ggcggaacgt aacaaggagg ccttatggaa
    12541 accacactcg taagccgtcg ttcttttctt cgtgtgacag ccttggccgg aggcggcatg
    12601 ttgctggctt cctacgtcga gccatttggc aaggtgctcg ccgcccagtt tggcccgccg
    12661 gttacgctag tcccggcttc attcatccgc atcgcgccgg atggcgttgt caccatcatg
    12721 gccaagaatc cggagatcgg ccagggtgtc aagaccatgc tgcccatgct gatcgctgag
    12781 gagctcgatg tggattggaa agacgtgcgc atcgagcaag ctgacctcaa tccgaagtat
    12841 ggtttgcaga tcgcaggcgg cagcaccgcc actccgatga actgggagcc tctgaggcag
    12901 gttggcgccg gtggccgaca gatgctgata gccgccgccg cgcagaagtg gagtgtgccg
    12961 gaatcggaat gcaccaccac ccccggccgc gtgcaccacg ctgccagtaa gcgctccgca
    13021 ggttatggtg aactcgccgc cactgccgcc acgctgaccc ctcctgatcc gaagtcgctg
    13081 aagatgaagg accccaagga ctacagaatc atcggcaaaa ccacgcccaa cgtagacatt
    13141 gaaaaaatcg tcaccggcaa gccgattttc ggcatcgact tcaccacgcc ggatatgctt
    13201 ttcgccaact ttcagaagtg tcccgtttac ggtggcaaag ttgccagcgc caatgtcgac
    13261 gaactcaagt ccgagcccgg cgtgaggcac gcgttcgtca tcgagccggt gggagattcg
    13321 agcgtattgt ctggcggcgt tgcagtcgtc gcggatactt ggtggcaggc gcgcgccgca
    13381 ctgggtaagc ttaaagtcac atgggatgaa ggtccgaccg ccaagcagag cagcaaactc
    13441 tatgcgacca cctccgtgga attggcccag aaagagccgg gtcaagtcct gcgcaaggat
    13501 ggcgatgcgg atgcggcctt tggcaatgcc gctcatgttg tggaagccgc gtattcgtat
    13561 ccgttccttt cgcacgtacc tctcgaaccg cagaactgct ccgcgtcttt taaggatggc
    13621 aagcttgagg tctgggcacc cagccaaact cccgcgaatg gtctgggaca aactgcgaaa
    13681 atttgcggca tagaccctaa ggacatcacc gttcatctca ctcgcgtcgg cgggggcttc
    13741 ggccggcgcc tcacggacga ttacatggta gaggcggcgt atatcgccaa gcaggttggt
    13801 gtgcccgtaa agctcttgtg gactcgcgag caggatatgg cccacgatta ttatcggccc
    13861 gccggttttc acttcctaaa gggcgcggtc ggggcggacg ggaaaatcgt cgcgtggcgc
    13921 aatcacttcg tcacgttcgg tgaaaatgac cgcttcgcgc agagcgccgg gctttccggc
    13981 gaggaatttc cgtcacgctt cgttccgaac ttctctcaac tttgctccat gatgcctacc
    14041 ggcgttccca cgggcgccat gcgcgccccc ggaagcaacg ccatcgcgtt cgtcatgcaa
    14101 tcatttatcg acgagttggc ccacgcggcc ggcaaagacc cggttcagtt ccggctcgat
    14161 ttgctcgcta ccacgcctct gccatccgct ccgccgccaa aaggcacacc catcgcgttc
    14221 gcgccggtgc cattcgatgc gccccgcatg aagggcgtgc tcgagatggt cgcggaaagg
    14281 tctggctggg gcagtcgcaa attgcccgga gacacagcga tgggcgtcgg tttccatttc
    14341 agccaccgcg gctatttcgc ggaggtcgcg gaagttcgcg ttaccggtgg ttccaacgtg
    14401 cgggtcaata aggcgtgggt cgccgctgac atcggcagcc atatcatcaa tccgggcgct
    14461 gcggagaacc ttacccaggg cgcgattatc gaagccatga gccatgccat gggctacgag
    14521 atcaccattg atgccggccg cgcggtgcag agcaatttca ataattatcc gcccgtgcgc
    14581 cttacgcagg ccccgccgga aatcgatgtc gcgttcctga agtcggacca tcctccgacg
    14641 ggccttggcg agccggcgtt gccgccgatg attccggcgc tcacgaacgc gattttcacc
    14701 gccaccggca agcgtgtccg ttcgctgccg ctccagactc agggtttcag ctgggcctga
    14761 tcgagcggaa aattagcagg gcgtggcgca ggcactcttg cctgcgcgca taagtcgggg
    14821 caatcggtaa gcgtacaccg caaaagcggc cgtgccgcct gcatactgaa tcactactaa
    14881 gcttccatct tcttgaagtt ttctcggcgg cttgctagaa ccttgaattc ctgatgacca
    14941 gccccgccta tctcaggatc gcaaccgaag aggcctacgc cccgcccgaa ttgttcgagc
    15001 gatataaaag gcttctcgaa gatggctcgc ataacgatcc cggttttgcg agcttcatgg
    15061 gtttttacct caataattcc gggcagcgcc tcaccgagat tcgcgcgcag attcaggatc
    15121 tcggcgagcg ccgcattcgc gacatggact caactggaat cgccaagcag atactctcac
    15181 tcacctcgcc gggcgtccaa atcttcgacg cgccaactgc cgtctcgctc gcaatcgcgt
    15241 tcaacgatca gcttgctgag gcaatcggca agcaccccga ccgctttgcg ggactagccg
    15301 cgatcgcgcc gcaggacccg tcggccgcag cgaaagaatt acagcgcgcc gtaaagtcgt
    15361 tgcacctcaa aggcgcgatc gtcaattccc acacgcgcgg cgaatatctt g
//
LOCUS       DATNDT010000516         4278 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_4192320, whole genome shotgun sequence.
ACCESSION   DATNDT010000516 DATNDT010000000
VERSION     DATNDT010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4192320"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            669..1247
                     /locus_tag="VN788_16800"
     CDS             669..1247
                     /locus_tag="VN788_16800"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HXU22239.1"
                     /translation="MKALAAVVLFVIGTSLQAGQNQYQISPAPAQAVGPAAQAKIDPA
                     KEADIRHLLDATGTAALMQQVMDNMEKSLKPVLENSFPPGEYRQELVNLFFEKFRSKL
                     DTKHLLDLAVVRYDEQFSDEDIKGLISFYQTPLGQKYVGALPKLTIALQQDAQALGQQ
                     TGRDSMIEVLAEHPELAKALQHAGQRGSPPSR"
     gene            1439..1726
                     /locus_tag="VN788_16805"
     CDS             1439..1726
                     /locus_tag="VN788_16805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22240.1"
                     /translation="MPEWEYAQADPSDELAQLSFFSMKKKQSGREIEFRITVREYVEQ
                     PASQHSRFFAEADKQVNQKTAAIVPSGWGHSVLEALSDCIRMIRQFPYEDS"
     gene            1745..2092
                     /locus_tag="VN788_16810"
     CDS             1745..2092
                     /locus_tag="VN788_16810"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU22241.1"
                     /translation="MKFAKKQIEPGIMVLEMTGRFLMGPDCRQVESEVAAHIARDQKS
                     FIFDLSGVEHIDSAAVGQIVKSYSSLKKCGGTLRLAGVSGMVDGVLKLTQVHKVIEIY
                     PTAQEAAKNFPRV"
     gene            2500..3300
                     /locus_tag="VN788_16815"
     CDS             2500..3300
                     /locus_tag="VN788_16815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HXU22242.1"
                     /translation="MTCVLAVANQKGGVGKTTTAINLAAALALKKKKTLLVDLDPQSN
                     ASIAFFAPDEVTSTMYDIFAEHPTEMAKIIRPTKDPNLFVAPARLALARLEQQLAGQF
                     DAPFKLKDALAPVAKSYDYVVFDTPPALGILTVNALVASTHLLVPIQAAYFAIEGTDD
                     LLETYGRIRSRPNPDLKVLGVVITLYDKRTTISRDTYEQIRATFGSALFKTRISKNVR
                     LEESPAYKETVFTYAPKSSGAEDYRKLAQEVIQRVEEDRVARHSQDAA"
     gene            3254..4105
                     /locus_tag="VN788_16820"
     CDS             3254..4105
                     /locus_tag="VN788_16820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HXU22243.1"
                     /translation="MSKRIGLPVTLKMRHDAHYVETLTSFSGASVGRMIPIDKIRPNP
                     DQPRKHIGDIRELADSIREKGVLEPLLVRYIPREDAYYIISGERRYHASQAAGLHELP
                     CIEKIADDAETLELALIENLQRKDLTAFEEADGLQRLADQFDYTHDDIAKKIGRARSS
                     VTETMSLRVIPEEVRRECVETGIATKSLLLQVARQPTEKKMHEMVHRIAQGGLTRDEA
                     RRARKEETESAPRPQPFVFDYEPEDASFHLRLQFRKSHVSAEELAAALRAILRRLESG
                     SVSSSAA"
BASE COUNT          952 a         1175 c         1245 g          906 t
ORIGIN      
        1 cgagttgaat gcggcgcagg tccagagtgc gtttaccaag tgggtccgcc ccgacgacct
       61 ggttcaagtg actgaaggcc ccaacccgca atagggctca ggcgtcgagc ggatctcgac
      121 cgtcgatcag cgccggaaac gaagagccgg ttcgcttgag ggccgcgcaa gaacctttaa
      181 ttattcagtt gtcaaagagc gatgttgctc tcgtcaagag ggcaaaacgt ccccaaaaat
      241 tgtcgatcag aactgacgac aatcgcagtg caccccgatt tggggctctc tcgaatgtga
      301 cgggtagact gtgtcgtcgc cctttgtttt ctgcgggtta gatcacattc tactcggcac
      361 atctgtcgat tagagattag ttgccgagct ggcgccgaag gcagactatt gcctgccctt
      421 gcgagggctg ctgactgcgc ctgaactgcg gtcggttgga ccatggtgtc catcttcgcg
      481 gatgaaagag atcgcggcaa gggtgaattc ctgtggactc tgcgttgtgg accgcaattc
      541 gaaggcagcg cggtccctcc ctcgcatttc tcctcctgcc tcagttaccg gcgcgacaca
      601 ggggccgaag cgctcggggc gggcacttgt caaatcagct agcatgaatt tatcgtcagg
      661 aggcttcgat gaaggctctc gctgccgttg tcctctttgt aatcggaacg tctctgcaag
      721 ccggccagaa tcagtaccag atcagtccgg ctccggccca ggcagtcggt ccggctgctc
      781 aggcgaagat cgaccctgcc aaggaagctg atatccgcca tctgctggac gctacgggca
      841 cagctgcgct catgcagcag gtgatggata acatggagaa aagcctcaag cctgttttgg
      901 agaattcttt cccgccgggc gaatatcgtc aagaattggt caatcttttc tttgagaagt
      961 tccgctccaa actcgatacg aagcatcttt tggacctggc cgtcgttcgg tacgatgaac
     1021 aattctccga cgaggacatc aaaggcctta tctcgtttta ccagacgccc ctaggacaaa
     1081 aatacgtggg cgcacttccc aagctgacaa ttgcattgca gcaggatgcc caggcgttgg
     1141 gccaacaaac ggggcgcgac tctatgatag aggtcctcgc agagcatccg gaactcgcaa
     1201 aagcgctgca gcacgccggg caaagaggat ctccgccatc gcggtagttt gccccacgga
     1261 caaaaaacct ctctgttcga tcacagttaa attattcctt ggacattagc ggaacttttg
     1321 agctcttcta tggcgcgtgg acgccgcggc agtgggaggg tccattgacg ccgcccgcca
     1381 tcgaaagcag cagtgtgtgg gttataatcc cgcagcttat tggaccagga ggagcgccgt
     1441 gccggagtgg gaatacgccc aagcagatcc ttccgacgaa ttggctcaac tttcgttttt
     1501 ttcgatgaag aaaaagcaga gcgggcgcga gatcgaattc cgaatcacgg tgagggagta
     1561 tgtcgagcag ccggcatcgc agcactcgcg ctttttcgcg gaagcagata agcaagtgaa
     1621 tcaaaaaact gctgcgattg taccgagcgg ctggggccat tcggtactgg aagcgctgtc
     1681 cgactgcata cggatgatcc gtcaattccc gtacgaggat tcctagcgcg cggagaccgc
     1741 cagcatgaaa ttcgcaaaga agcagattga acctggaatc atggtgctcg aaatgacggg
     1801 ccgctttttg atgggccccg attgccgcca ggtggagagc gaagtggcgg cgcacatcgc
     1861 acgcgaccag aagagtttca tcttcgatct ctcgggcgtc gagcacattg acagcgcggc
     1921 ggtggggcaa atcgtcaagt cctattcgag tctgaagaag tgcggaggga cgctgcggct
     1981 ggcaggcgtg agcggcatgg ttgacggagt gttgaagctg acccaggtgc ataaagtaat
     2041 tgagatttac cccacagcgc aggaagccgc aaaaaacttc ccgcgggtgt agcaccggtc
     2101 ggcccgggct gttcaagcgt ctatccgtag aattgtcggg ctggatggaa ctatcgcatg
     2161 gccgtggatt ccgagaacat tccagcgacg gccacctatt gtttgtttga tttgcaggta
     2221 gaggtacgct cggtagcctg cgactggaag aggggagaga gttacgtgct tccggttggg
     2281 gagtccacag cgacgccctt cagcgtcgta cagaactggg atgcggggtt gaagaaatga
     2341 caatgctttc cggacagatc gtttttgatt gaaattgcaa attttgcttg ctttgagtgc
     2401 taaaccgtat gtacatttgg cgctactcat ggtagcggca cactttctgg gttctacata
     2461 tgggcctgga gttgctgctc gggagcgagg ggcacgcgct tgacctgcgt cctggctgtt
     2521 gccaaccaaa agggcggcgt cggcaaaaca acaactgcca tcaatttagc cgccgcgctc
     2581 gcactgaaaa aaaagaaaac acttctcgtc gatctcgatc cgcaatcgaa cgcgtcgatc
     2641 gctttttttg cgccagacga ggtgacgtcc acgatgtatg acatcttcgc tgagcatccc
     2701 acggagatgg caaagattat ccgtcccacg aaggacccga atttgtttgt ggctcccgcg
     2761 cgactggcgc tggcaaggct ggagcaacaa ctcgccggac aatttgacgc tcccttcaag
     2821 cttaaagacg cgttggcgcc ggtggctaaa agctacgatt acgtcgtgtt cgatacgccg
     2881 cccgcgctgg ggattcttac ggtgaatgcg ctggtcgcgt cgacacattt gctcgtgccc
     2941 atccaggcgg cttatttcgc catcgagggc acagacgatt tgctagaaac ctatgggcgc
     3001 atccgctcgc gtcccaatcc agacctgaaa gtgcttggcg tggtgatcac gctctacgac
     3061 aagcgcacta caatttcacg cgacacctac gagcagattc gcgccacgtt cggcagcgcc
     3121 ctcttcaaga cgcgcatcag caagaatgtc cgcctcgagg aaagcccagc ctataaggaa
     3181 accgtgttca cctacgcgcc aaaatcttcc ggcgcggagg actacagaaa acttgcccag
     3241 gaggtaatac aacgtgtcga agaggatcgg gttgcccgtc actctcaaga tgcggcatga
     3301 cgcgcattat gtggagacgc tgaccagttt cagcggcgcg tctgtcggcc gcatgattcc
     3361 catcgataaa attcgtccga atcccgatca gccgcgcaag catatcggag atatccgcga
     3421 actggccgat tcgattcgcg aaaagggcgt gctggagccg ctgctggtcc gctacattcc
     3481 ccgggaagat gcgtactaca tcatatcggg cgagcgtcgc taccacgcct cgcaagcggc
     3541 agggctgcac gagcttcctt gtatcgagaa aattgccgac gacgcagaaa ctctggaact
     3601 ggcgctgatt gaaaatttac agcgcaagga tctaactgca ttcgaggaag ccgatggttt
     3661 gcagcgcttg gcggatcaat tcgactacac gcatgacgat attgcgaaga aaattggtcg
     3721 cgcacgttcg tcggtgacgg aaacaatgtc tctgcgcgtg attcccgaag aggttcgccg
     3781 ggagtgcgtc gagaccggca tcgccactaa atcgctgctc ctccaagtcg cccgccaacc
     3841 caccgaaaag aagatgcacg agatggtgca ccggatcgct caaggcggat tgactcgcga
     3901 cgaggcccgg cgcgcccgca aggaggaaac ggaatccgcg ccgcgcccgc agccgttcgt
     3961 tttcgactac gagccggaag atgcatcgtt ccatctgcgg ctgcaattcc gtaagagcca
     4021 cgtcagcgcc gaggagctgg ccgcagcatt acgcgcgatt ctgcggcgcc tcgagtccgg
     4081 ctctgtctct tcctccgccg cttagttgtg cgaggcggcg ctcggggccg ttagcgaatt
     4141 ataaatcgtt tggataaacg cgtcggtatt gacgaaggtg ccggagtatt tctgccacgg
     4201 cgcgagaatc ttttcctctt tcatctgcgc gagtgtcttg tgatcgtcga gcgccttccg
     4261 caccgcggcc gtggtgtc
//
LOCUS       DATNDT010000517         8577 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1699646, whole genome shotgun sequence.
ACCESSION   DATNDT010000517 DATNDT010000000
VERSION     DATNDT010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 8577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8577
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1699646"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VN788_16825"
     CDS             <1..469
                     /locus_tag="VN788_16825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22244.1"
                     /translation="SSDTGTTGGGGTTTTGSSAGADAGSSTSTPVWHIPDHESKTAVP
                     QVAQSAAVTTTTETATTGTTTSSTNAAHDSGAGFFSSSSDQFVFHSMSIGSSGVDRST
                     HPQSIETAQAAVTNVQESASLASSADLHGSSDMSFAMHDIFGLHQMHVDQLFS"
     gene            complement(488..1963)
                     /locus_tag="VN788_16830"
     CDS             complement(488..1963)
                     /locus_tag="VN788_16830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22245.1"
                     /translation="MKAPSSIPGQFIILAGQDEVHLGPNPLRDGWTRYEVGAFRVFAA
                     PEVPVIRCKNADGSTFGIFLGEPLNLEESRFLSGDELFGQLPPGGAEIAQWIEKHVYR
                     LAGRYIFVLSTRDVHRVYLDVDGFLPLVYDPSLRIAGATAGAIYDSNQYLSSFDSELF
                     EALNLLNGGWFPAGLTAHHGLRRLMCNFYLDLESWTASRHWPTGGIEAADDAHSTLKR
                     ISASTQRTLVTYLEQRSCCMALTGGNESRLLLSLARSNAQSIPFICWYDKQGARDAEI
                     SENLAAKFGLNLTFLGFPSVTEDEATEWLARIGYCATENRYRYKVSARLSGFDCMIGG
                     LGGEIGRGFFWRRGDENRSLTPRDIYKRLALPPHPRAASAVDSWFQTLPTCEPLLQLD
                     LAYLEVRMSCWAAVQAYGGIGPRQVYPLVSREVYSQMLALPPSWRRNNRWVTEIIGRH
                     WPDLLAVPINNLGPLREAARLAARVARKPSLIVRRWRRRFA"
     gene            2051..3541
                     /locus_tag="VN788_16835"
     CDS             2051..3541
                     /locus_tag="VN788_16835"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HXU22246.1"
                     /translation="MTQATSGARTPRQSQPTNSRPASGRKAYLIALGVAQGSAFIRSI
                     VLARLLGPEQMGLAAIIVVTAQFFDSITDTGNDRFLIQDRNGASADSLQLVHLVAILK
                     GTLIALLLIILARPIAWFTAAPETASALIGLALVPFLNGFMNYGFRVAQRHHQFGPEA
                     KVMLVSEIAGLAATVVAAFELRNFTAILYGLAFRAAVGAIASHLVASQSYRPRFSMQT
                     AKRLWRFSAPLALNGLLIFVATQSDRVIISRHLGLSELGRYTVILLIGLYPSLTLMKF
                     VSALYLPRIAGARDEPPNMRRISDQLEGGTVLLAAFVAVGFSIAVPSMLPFIFGSKFM
                     STTLSITLLGLVVSWRMLKTAPTVVAIAIGRTTILFANNLLRLSGILGAIAGLRLIGG
                     IGGVAAGLIIGEVIANVAATIMVCRSTHWSFAGAARRYGFALIVGAALIFRGWASDQR
                     FVMLATIATTISVAALLIGVLRERRTVNEFVILMRRSMKTPSRGTA"
     gene            4228..5223
                     /locus_tag="VN788_16840"
     CDS             4228..5223
                     /locus_tag="VN788_16840"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HXU22247.1"
                     /translation="MAVWLSLVGAWEWRLGRVPWAGHIPSFLQINDDAVLRTLAGTTR
                     SGAVAHRVEATFAVSLGLGEYLALVTPLIIHFSLEGTSRFPRWSAALILPLVLFTTIN
                     SGARIGTVGFCASVILYGGLWAVRRWRIDRRSILGPAMTLGYPAIAAALLSITFISGR
                     LRQIVWGGHNTDPSNQARIDQFHMGWPKVLSAPLGHGVGRGAQVLGYYNGDGVLTIDT
                     YILRLALEYGMIGLFVYYLFFFSAVARGIPGALRATDESRKLLMPLSVSILVFLLGKS
                     VYSSEYAHPIPFMMVGAICALLTGNSAPIPGGRAPFGRAIRNARIRSTNVLSNHD"
     gene            complement(5881..6672)
                     /locus_tag="VN788_16845"
     CDS             complement(5881..6672)
                     /locus_tag="VN788_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22248.1"
                     /translation="MIRGVKAATQAALGRIYQNNYPELIRWGAVERPHYAYIVYQAAE
                     LARRLGVQRISVIEFGVAGGRGLLNLEHHAKRVSQLTGVNIDVYGFDTGEGLPPPIDY
                     RDLPYIWKSSHFRMDQHALQAKLKNARLVLGNVADTVSSFVEQYAPAPIGAIAFDLDY
                     YSSTIEAFRIFNIPEARRQPRIFCYFDDIFSSDLGHVGPAVGVPLAINEFNRNNTESV
                     LSELNHLEFEYRPVRRWHRQIYSFCDFDHPRADEYIIDGDRQLEL"
     gene            complement(7686..>8577)
                     /locus_tag="VN788_16850"
     CDS             complement(7686..>8577)
                     /locus_tag="VN788_16850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008068857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HXU22249.1"
                     /translation="DIIACLGALSRATYADFEVVICENGGEHSYRSLVAALPARLPGG
                     QPVRTICAAENLGYAGGVNVCVRASPHADAWWILNPDTVPDSTALEYLVQRLRAGDCE
                     LVGGILYGTNGRIQTLGGRWRPWLARAEAIGRGQSADRVPLQSLERLIDFVSGASMLV
                     SRRFLDVVGPMREDYFLYCEEVEWCLRGHAHGMRIALAPNARVLHHQGSTTGSALPVR
                     RRSWMQVYLDERNKMLVTRDCFPSHITVAISAALLLLLIRYTRHTAIRQLSFALQGWW
                     AGVTDRRGPPSIGLRRESNT"
BASE COUNT         1921 a         2390 c         2260 g         2006 t
ORIGIN      
        1 cagcagcgat accggaacaa cgggcggtgg cggcacgacg accaccggca gctccgcggg
       61 cgctgacgca ggaagttcga cttccacgcc tgtctggcac attccagatc acgagagcaa
      121 aacggcggtg ccccaagttg cccaaagcgc ggcggtgact acaacgaccg aaacggcaac
      181 gacgggaacg acgacgagca gcacaaatgc ggctcatgac agcggcgccg gctttttttc
      241 ctccagttca gaccagttcg tcttccattc gatgagcatc ggctcttccg gcgtcgatcg
      301 cagcactcac ccgcagtcga ttgagacggc ccaggcagcg gtcacaaacg tgcaagaatc
      361 ggcctcgttg gcgtcatcgg ccgatcttca tggcagctcc gacatgtctt ttgcgatgca
      421 cgatattttc ggtctccatc aaatgcatgt cgaccaactc ttctcatagg tcgagcgcga
      481 cgtatttcta agcaaatcgc ctgcgccatc gtcgcacaat gagtgagggc ttgcgagcga
      541 ccctggccgc caaccgcgca gcctctcgga gtggcccgag gttgttgatt ggaacagcca
      601 ggagatcggg ccaatgccgg ccgataatct ctgtgaccca acgattgtta cggcgccacg
      661 aaggagggag tgccaacatc tgactgtaca cttcgcgcga aactagcgga tacacctgcc
      721 ttggcccgat tccaccatat gcctgcaccg ctgcccagca gctcattcgc acttctaggt
      781 aagccaaatc cagctgaagc aaaggctcgc aagttggcaa tgtttgaaac caactatcta
      841 ctgcagaagc cgcccttgga tgaggcggta gcgcaagtcg cttataaatg tcccggggag
      901 tgagcgaacg attctcgtcg cctcgtcgcc aaaaaaagcc gcggccgatt tctccgccta
      961 agccgccgat catacaatca aatccagaaa gccgcgcgga tactttgtaa cggtatcggt
     1021 tctcagtggc gcaatatccg atccgtgcga gccattcggt tgcttcgtct tccgtgacgg
     1081 aaggaaatcc gaggaaagtc agattgagac cgaatttggc agcaagattc tcgcttattt
     1141 cggcatcgcg cgcaccttgt ttatcatacc agcaaatgaa tggaatggac tgcgcgttgc
     1201 ttcgtgccaa ggaaagcagc aaccgactct cgttgccgcc agtcagagcc atacaacacg
     1261 aacgttgctc caaatatgtg acaagtgttc tttgtgtact tgctgatatg cgcttcagcg
     1321 tcgagtgggc atcgtcagcg gcctcaatac ctccagtcgg ccaatgtcgg ctcgctgtcc
     1381 aactttccaa gtcgagatag aagttgcaca tcaggcgtcg caaaccgtga tgcgcagtca
     1441 agcctgccgg aaaccagccg ccattgagca ggttcaaggc ctcgaagagt tccgaatcga
     1501 agctgctcag atactgattg gaatcgtaaa ttgcgcccgc ggtggcgcct gcaattcgca
     1561 gagatggatc gtaaacaaga ggcagaaagc catcgacgtc gagataaacc cgatgaacgt
     1621 ctctggtact aagcacaaat atgtagcgac ccgccaaccg atacacgtgc ttttcaatcc
     1681 attgggcgat ctcagcccca cccggcggca gctgcccaaa taattcgtcg cccgagagga
     1741 aacgagactc ttcgagattc agaggttcgc ccaggaagat gccgaacgta gatccgtccg
     1801 cattcttgca gcggatgacc ggcacctccg gcgctgcgaa cacgcggaat gctccaactt
     1861 cgtaccgtgt ccaaccgtcc cgcaatgggt taggacctaa atgcacctcg tcctgcccag
     1921 ccagaatgat aaactggccc ggaattgatg aaggggcctt cacaacggat tgcctatatc
     1981 gatccagacg catggccccc cgcttaactc gaaaaatgtg aatcgctatg tttcttcacc
     2041 gggagcaaac ttgacgcaag caacttcggg cgcccgaacg ccgcgccagt cacagcctac
     2101 caattcgcgt cccgcaagcg gccgaaaagc gtatctgatt gcgcttggcg tcgcccaggg
     2161 atctgcattt atccgttcca ttgtgctggc gcgtcttctt ggcccggaac agatggggct
     2221 cgccgcgatc atcgttgtca cagcgcaatt cttcgactcg atcactgaca ccggaaacga
     2281 tcgattcctg atccaagatc gaaacggcgc ctctgcagac tcattgcaac tggttcatct
     2341 cgtcgcgatt ttgaagggga ctctgatcgc acttctatta attattctgg ctcgacccat
     2401 tgcgtggttc accgcagcgc ctgagacagc aagcgctctg attgggctcg ccctcgtccc
     2461 gttcttgaac ggcttcatga attacggttt ccgagttgct cagcggcatc atcaattcgg
     2521 acccgaagcc aaagtcatgc tggtttccga aattgccgga ttggcggcga cggttgtcgc
     2581 agcatttgaa cttagaaact tcacggccat attgtatggg ctcgcattcc gcgccgccgt
     2641 cggtgcaata gcttcgcacc tcgtcgcctc gcaaagctat cggccgcgct tttcaatgca
     2701 aaccgccaag agactgtggc gtttcagtgc accgttagcg ctcaatggac ttctaatctt
     2761 tgtcgcgaca caatcagacc gagtgatcat tagtcgccat ctcggcctgt cggagctggg
     2821 ccgctacacc gtgatcctgc tcatcggact ctatccgagc ctgacattga tgaagttcgt
     2881 tagtgcactg tatctccccc gtatcgcagg cgctcgagat gaaccgccta atatgcgtcg
     2941 gatatccgac cagctcgaag gcggaaccgt cctcctagcg gctttcgtcg cggtcggctt
     3001 ttcaatcgca gtgccctcca tgttaccgtt cattttcggt tcgaagttca tgagcacgac
     3061 gctttcgatc acgctgttgg gactcgtcgt atcatggcgc atgttgaaga ccgctccaac
     3121 agtcgttgca atcgccatcg gccgcacgac aatcttgttt gccaacaatc tgttgaggct
     3181 cagcggaatt ctgggtgcaa ttgctggcct acgactcatc ggagggatag gtggtgtggc
     3241 ggcgggcctc atcattggcg aagtgatagc aaacgtggca gcaacaatca tggtctgtcg
     3301 ttcgactcac tggtcattcg ccggcgccgc tcggcgctac ggtttcgctc ttatcgttgg
     3361 agcggcattg atatttcgtg ggtgggcatc ggaccaacgt ttcgtaatgc ttgcgactat
     3421 cgcgaccacc atatccgtag ccgcgttgtt aattggcgtc ctgcgggagc gccgaacggt
     3481 gaacgaattc gtaattctga tgcgcagaag catgaaaact ccaagcagag gaacagctta
     3541 aatttgtcat ccgccgaagg gcacgcattg aagggacgat ccttgatctc acgaatacag
     3601 cgaccaggaa taaagccaga aacgcgactt ccgatctatg ttcccgatcg caggtccggt
     3661 cggcgtcgga ggctacggaa gtggtgttgg atagctttgg ttgtcactgc tttcctctac
     3721 ggggcatgct tcgcattctt tcctcctgct tttctgccgc ctctcctaat accgcttgta
     3781 atcatagcag ggctcgtgat ctgggccttg ccggccacgc atcggtctcc gcctatctgg
     3841 attatgcggt cactattcat tagcttcgtt gttgcactcg ttgcatggcc gaattacctc
     3901 gccatctctc ttcctggttt gccatggatt acgctgagtc gactctttgt atttccactt
     3961 gtcacgattt tcttaggcta tatgtcggtc tccaagacag tgcgtgacga tactcgagcc
     4021 gtaatcagtg ataatataat aataattcgg cttctgcttg cattcgttat aattcaacta
     4081 atatccatac cattttcagg cgacccattc gactctttcc aaaaatttct tatcgatcaa
     4141 gtctactgga ctgccatttt tttcttgtct tgctacttat ttcgtgaccc gcgattcatt
     4201 actcgatgcg cttggatctt ggtggcgttg gctgtttggc taagcttggt tggggcatgg
     4261 gaatggcgcc tagggcgagt tccatgggcg ggccacatcc catcattcct gcaaattaat
     4321 gacgacgccg tactgcgaac gctcgccggc acgacgcgat cgggtgccgt cgcgcatcgg
     4381 gtcgaggcaa ccttcgctgt aagcctcggc ttaggtgagt atctggcttt ggtcactccg
     4441 cttatcattc acttcagttt ggaaggcact tcacgatttc cacggtggag cgctgcttta
     4501 atacttccgt tagtactctt cacgacgatc aactccggtg ccaggatcgg aaccgtaggt
     4561 ttttgcgcgt cggtgattct ctacggcgga ttgtgggcag ttcgccgctg gcggattgac
     4621 cggcgaagca ttcttggacc ggcaatgacg ctgggctatc ctgcaattgc ggctgccctt
     4681 ctttccatta ctttcatctc aggaagatta cgccaaattg tttggggagg gcacaacacg
     4741 gacccaagca atcaagcacg aattgaccaa ttccacatgg gttggccaaa ggttcttagc
     4801 gccccgcttg gccacggggt tggacgaggt gcacaagtgc ttggctacta taacggagat
     4861 ggggtgctaa ctattgatac gtatatactg cgcttggcac ttgaatacgg tatgattggg
     4921 ctgttcgttt attacctctt tttcttctcc gcagtagctc gaggaattcc gggagcgctc
     4981 cgagctaccg acgagtcaag aaagcttctt atgccattat ctgtttcgat cttagtgttt
     5041 ctgctaggaa agtcagtcta cagttcagaa tacgcgcacc caatcccgtt catgatggtt
     5101 ggggcgatct gcgcgctgct cactggcaat tcggctccga taccaggcgg ccgcgcccca
     5161 ttcgggcgtg cgatcaggaa tgcccgaatt cggtcaacaa acgttttgtc taatcatgat
     5221 tagcgacttt gtcagaccgg caaatcgttc tcgaacgaga gtgccgtcag ggaacaacct
     5281 tctcatctgt aacgaactca agagctgcgt acccgcaaca accctttccg cggcctccgg
     5341 cgtcgggatt cgccctgcgt gcgcaatggg taaatactgc aatagcgacg ctcgcatgcc
     5401 ggttggcaac caatggaaaa atggaacctt gtagaagtgg gggtcaagtg gaaaccacca
     5461 attcggagac tgaacgtaat aactcggagc aagccgacga acttcatgag cgaacatgcg
     5521 cattctgtcc cacgtcccaa catgttcaat tacagagttt gaatggacga aatcgaatgc
     5581 catgtccccg catccgacgt tgcaagcatc gccaaccagc acctggaatg ggccttcgcc
     5641 gagctcgcat tgatctgaat tcagaattgt gacttgaaag ccgttgtcct gaagaaacgg
     5701 caatccaata ttctgccagt actcttgggt tccacccaga tctagtacgc ggcgtcctgc
     5761 ccgcagcatg atttgccgca gcaagactgc tcgacgcgct ctgaagtgag atgagaagga
     5821 tgtcggatcg cgcgggtcat ttaacgtcac agtaaattgc tcgcttctta taagaaaacg
     5881 tcagagttcc agttgtcggt cgccgtcgat gatgtattcg tcagctcgcg gatggtcaaa
     5941 gtcgcaaaaa ctgtatatct ggcgatgcca acggcggacg ggccggtact cgaactcaag
     6001 atgattcagc tccgaaagaa cagattctgt attgtttcga ttgaattcgt taattgccag
     6061 cggaactcca actgccggac cgacatgtcc gaggtcactt gaaaatatgt catcgaagta
     6121 gcagaaaatc cttggctgtc gacgtgcctc cggaatattg aagatccgaa aagcttcaat
     6181 tgttgagctg taataatcca gatcgaaggc aatagcgccg atcggtgcgg gagcatattg
     6241 ttcgacaaag gaactgacgg tatcggcgac atttccgaga acaagtcgcg cattttttaa
     6301 tttcgcttgg agcgcatgtt ggtccatgcg gaagtggctc gatttccaaa tgtagggcag
     6361 atcacgatag tcaatcggtg ggggcaaccc ttcaccagtg tcgaagccat acacatcgat
     6421 gttgacacca gtgagctggc taacccgctt cgcatgatgt tccaaattta agagtccccg
     6481 acctccagcg actccaaatt cgatgacgct gatcctctgc acacccaacc gccgagcgag
     6541 ctcggcagct tgatagacga tgtacgcgta atgagggcgc tcgaccgctc cccatcgaat
     6601 tagttctgga taattgtttt gatagattcg accgagtgct gcttgcgtgg ccgccttgac
     6661 tccgcggatc atctatgccc cctccatcca ggtaatcggt actcctcgcg ccgggtgagc
     6721 gtcaaatcga tgacttactg cctaacctgt ggcatattaa gttgagagcc acgtagaggg
     6781 tgagcatgca atgcaagcga cggcggacct tgagaaaccg ctaccagaaa gagcggagcc
     6841 acttctacgg ctgtcccgtg ctgacggctt ctttgaattt agtgcagacc aatgccgtaa
     6901 tgctggaatg cagctgcata acaaatatct cgaaaacgat ccgttcccgc acattgtgat
     6961 tgagaacttc gtggatctga acgttcttcg tgaggtaaac gctcagttcc ctatgccacg
     7021 ggccggtcgc ttttcagata agttttctca gctcaagacg gggtattcac tcgatttgat
     7081 taggagtgcg tatatccacg atcttcttcg tgcttttaat tcggcagcgt tccttgactt
     7141 cttggaggcc ctaaccggca ttcatggtct cgtagatgac agcagattca ctggcggcgg
     7201 tcttcacgaa acacgccgtg gcggtcatct ttcaatccat tcagatttca acatccatcc
     7261 aatcacgggg ctcagacggc gtttaaatct gatattgttc ttgaatgaca catggaatga
     7321 acgatgggga ggatttttgg agctatggga tcaaactatg tccacgtgcc gacggtccgt
     7381 tcttccccag ctggggacag cagtcatctt taatacagag gggaagaatt accacggaca
     7441 cccggatcct ctcgatacgc cccctgaggt gactcggcga tcgatagcgt tgtattatta
     7501 tacgaacccg cgcggcatca tagttccacg aacgacagtc ttccaggctc gtccctcaag
     7561 aagcgagttt gcaacgccgc tctcacagcg gctgcaagaa acagtccggc tcttgctacg
     7621 caggcgtgag cgataacgcc gtggattcat tgggggcagg cccagatgtt attcggccat
     7681 tggtattagg tgttgctctc acggcgcaaa ccaattgacg gaggcccgcg tcgatctgtc
     7741 actccggccc accaaccctg taaggcgaat gagagttgcc gaatggccgt gtgccgggtg
     7801 taacgaatga gtaacagtag taatgccgcc gagatcgcga cggttatatg ggaagggaag
     7861 caatcgcgcg tgacgagcat tttattgcgt tcgtcaaggt acacctgcat ccaggatctc
     7921 cgtcgcactg ggagcgccga cccagtagtg ctgccctgat gatgcaacac tctcgcattc
     7981 ggcgccaggg caatgcgcat tccgtgtgca tgcccgcgga ggcaccattc gacctcctcg
     8041 caatatagga aataatcctc gcgcataggt cccacgacgt cgagaaaccg gcggctgacc
     8101 agcatcgatg ccccgctgac gaagtcgatc agccgttcaa ggctctgtaa tgggacgcgg
     8161 tccgcagatt gccctctacc gatcgcttcg gctctggcaa gccagggccg ccatcgtccg
     8221 ccgagcgtct gaattcgccc gttggttccg tagagaattc ctccaacgag ctcacaatcg
     8281 ccggcacgca ggcgctggac cagatactca agcgcggtgc tatccggcac cgtgtcggga
     8341 ttgagtatcc accacgcatc ggcatggggc gaggcgcgaa cgcaaacatt cacgccaccg
     8401 gcgtagccga ggttttcggc agcgcagatg gttcggacgg gttgcccgcc cggtagtctt
     8461 gcaggcaatg ccgcgaccag cgagcggtaa gaatgctctc caccattctc gcagatgacc
     8521 acttcgaagt cggcgtatgt cgcccgggag agcgccccga gacaagcaat gatgtcg
//
LOCUS       DATNDT010000518         4714 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2585736, whole genome shotgun sequence.
ACCESSION   DATNDT010000518 DATNDT010000000
VERSION     DATNDT010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4714
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2585736"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..720)
                     /locus_tag="VN788_16855"
     CDS             complement(<1..720)
                     /locus_tag="VN788_16855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22250.1"
                     /translation="MRLSPVKFAIRGLAVALACLAVPEFSYAQMSRGGAQHPGPVTSG
                     TDSMQTELHMLTDVQRQGIDRKEENAYKAFYNQKDELDKKIQLGQAFLQKYPKSPLAE
                     AVDAGLVNAYVAKQDWKDVYASADSALALKPDDVDVLTTVGWVIPHVYQPDDPDGPAE
                     LDKAETYEKHAMEVMATMPKPSHLSDAQFAAAKAQKASEAHSALGLVYYRRADFANSA
                     KELDQAMHGEPNPDATDLYVYG"
     gene            complement(780..2354)
                     /locus_tag="VN788_16860"
     CDS             complement(780..2354)
                     /locus_tag="VN788_16860"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU22251.1"
                     /translation="MRLFAWVLSAAIFFGAFIAARSPEAQGQTAAQRNFGISVQASPQ
                     IFATMCALDAAGLDADESALSEMPARLALRGDLLKMQGTATEALRQFYKDHVLESPAE
                     TLSRYITFALVAGPPPAFELPAGFDQLPPDVLTIDGFQKVLEDFYQEAHLDLRWAKIE
                     PEYEPAVERYRASLRRIVTVSDAYLREISKPDGRSFTVYVEPLVGARTNFRNFGDRYA
                     IVVGGDAGARANAIQHAYLHFMLDRFVLRQRPVLDRKSSLLNVAAAAPRLPMEYRGDF
                     VAFTDECFVKAVELRLRHLTGAPLEAALQDADQSGYILVRPLVAGLQKFEKAEPAMSY
                     YFPDLIAGIDFDAEHQRAQRIKFAAAQPNAEADEIHGPGNGNASELERWLAEGNREIA
                     NKDAAGATATFEAALAKYPNDARVEYGLAIASVISGDGDRAKELFEKVVSAPASTGKD
                     AQEAASSDPSAIAWSHVYLGRIHDLGDERDLALNEYRAALAVNGAPEAARVAAQNGIN
                     AAYQPPPSAGKKTEPE"
     gene            2661..4703
                     /locus_tag="VN788_16865"
     CDS             2661..4703
                     /locus_tag="VN788_16865"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HXU22252.1"
                     /translation="MALPFAFAFMLAAAPLFAASQWSTVGGDSGNTRYSSLTQINAQN
                     VTKLGAAWMSEKITPPPSSRGVPVVDKDLMFLTAPPFVYAVNIGTGQIAWKYRAAPGE
                     GGLFGPPGSPAREGVAVGEGLVFVGLSDSHLIALREDTGELVWKTSLAAPSGEPTGAP
                     SGAPLYADGLVSIGTNADYGYRGQIVALDAKTGHEAWRFFVVPSPGEPGSNTWPKNNN
                     AWKHGGGAIWLVGTYDPALGLVFYATGNAVPQYGGEHRPGDNLYTDCVVALDSKTGKL
                     RWHYQVVHHDIWEADVAEAPVLFDAQVNGQSRKAVAAMRTDGYLFMLDRVTGKPLGKI
                     EERRVQQDAFQKTAATQPYPVGADHVLPDCDYWKTQPLPRGFELGCFFTPAAAEKPNI
                     LAPVYGMRATPMAFDPQTQFFYVTGDAALQWFRRADDPDYFSLSFSQRVPGLNKLGYG
                     VLAAIDTRTDKIAWKKEFHDERPSGALATAGGLVFQMPGDGNLQAYDATNGNLVWQFQ
                     TGALGGSPPFAFESGGEQYVATIVGGNVWAFKLGGTLPARPAPPLPPQPEFAGPITDT
                     ATIQTTALARDMGLTGSHYLGDEYAFDPYRARVKAGTEVRWRNNGAVTHTIVAVDGSW
                     TTGPLQPLDIGIVRFDKPGTYTYICKDHPWVYGQIIVEPAAPEEQQQSKLSGKVEP"
BASE COUNT          934 a         1459 c         1333 g          988 t
ORIGIN      
        1 gccgtaaaca tacaaatcgg ttgcgtcggg attgggctca ccgtgcatcg cctgatcgag
       61 ctctttcgcg gagtttgcga aatccgcgcg ccgataatag acaagaccca gtgcgctgtg
      121 cgcctccgaa gccttttgcg cttttgcggc cgcaaattgt gcatcggata aatgcgaagg
      181 cttcggcatg gtcgccatca cttccatcgc gtgtttctcg tacgtttccg ctttatccaa
      241 ctcggccggc ccgtccggat catcgggttg gtaaacatgc gggatcaccc agccgaccgt
      301 agtgagcacg tccacgtcgt ccggcttcaa cgccagcgcg ctatccgccg acgcgtacac
      361 gtctttccaa tcctgcttgg caacgtacgc attcaccagt cccgcatcca ctgcctcggc
      421 aagcgggctc ttcgggtatt tttgcaggaa cgcctggcca agttggattt tcttgtccaa
      481 ctcgtctttc tggttgtaga aggccttgta ggcattttcc tcttttcgat cgattccttg
      541 ccgctgcacg tcggtgagca tgtgcaattc ggtctgcatc gaatctgttc cagacgtcac
      601 cgggcccggg tgctgtgctc cgccgcggct catttgtgca taagaaaatt ctgggactgc
      661 aaggcacgcg agcgcaaccg ccagccccct aattgcaaat ttcacgggtg aaagtctcat
      721 tgggattgcg cctccttctt tcgccacatg cctcccgttt ccatgcgacg ccaaagtcat
      781 cactctggtt ccgttttctt gccggcgctc ggaggcggtt ggtaagcggc attgatgccg
      841 ttctgcgcgg ccacgcgtgc agcctccggc gctccattca ccgccagcgc cgcgcgatac
      901 tcattcaatg ctaggtcgcg ttcatctccc agatcgtgta tgcgtccaag atacacgtgc
      961 gaccaggcga tcgccgacgg atcggatgat gccgcttcct gcgcgtcctt tccggtcgaa
     1021 gccggagccg ataccacttt ctcgaaaagc tccttggctc ggtcgccatc cccggaaatc
     1081 accgacgcga tcgccagtcc atattccacc cgggcatcgt tcggatattt tgcgagcgcc
     1141 gcctcgaaag ttgcggtcgc gcccgcggcg tccttgttgg cgatttcgcg attgccttcg
     1201 gccagccagc gctccagttc ggaagcgttc ccgtttcccg gcccatgaat ttcgtcggct
     1261 tcggcgttcg gttgcgcggc cgcaaacttg attcgctggg cgcgctggtg ttccgcatcg
     1321 aagtcgatgc ccgcgatcaa atcggggaaa tagtacgaca tcgccggttc ggccttctcg
     1381 aatttctgca gccccgcaac caatggacgc accagaatgt atccagattg atccgcatcc
     1441 tgcagcgctg cttcgagcgg agcacccgta aggtggcgca aacgaagttc caccgccttg
     1501 acgaagcact cgtcggtaaa cgctacgaaa tctcctcgat attccatcgg cagccgggga
     1561 gcggcggcgg caacattcag aagcgaactc tttcgatcca gcaccggacg ctgacgcagc
     1621 acgaaccggt ccaacatgaa atgcaaatat gcgtgctgaa tggcattcgc gcgcgcgccg
     1681 gcatctccac ccacgacgat cgcgtagcgg tcgccgaagt ttcggaaatt cgtccgcgcg
     1741 ccaaccagcg gctccacgta caccgtaaag ctgcgtccat ccggcttgga aatttcccga
     1801 aggtacgcgt ccgataccgt cacaatccgg cgcagagacg cgcgatagcg ttccacggcc
     1861 ggctcatact ccggttcaat cttcgcccag cgcaaatcca gatgcgcttc ctgatagaag
     1921 tcctccagca ctttctggaa gccgtcaatc gttagcacgt ccggcggaag ctgatcgaaa
     1981 ccggccggca attcgaatgc cggaggagga ccagcaacca gagcgaaagt gatgtagcgc
     2041 gaaagtgttt ccgcaggact ttccaggacg tggtctttgt agaattgtcg cagggcttcg
     2101 gtcgcggttc cctgcatttt cagcagatcg ccgcgcagcg caagcctcgc cggcatttcg
     2161 gaaagcgcgc tttcgtccgc gtcaagcccg gccgcatcaa gcgcgcacat ggtggcgaaa
     2221 atttgcgggc tcgcttgcac gctaatgcca aaattccgct gagcagcggt ctgcccctgc
     2281 gcctcgggtg acctcgccgc gataaaagcg ccgaaaaaaa tcgcggcgga taatacccac
     2341 gcgaaaagcc gcatcggatt tcggacacgc ggagttctca acccaaacct ctctccggaa
     2401 tttcatccgc cggcatggct ttcgaatgta cgggcgaacg ccaggcggtt ctcaaactct
     2461 aacaaagaag ccaaaaaatc ttaaatggtt tgctgaggtg tcctcagtac attctctcgt
     2521 ctgcaatctc ctcctcactg cgcgcgaact tgctgaatac tgcggaaagt tgcgttaaac
     2581 tcgcgctgta atccataggt cctcgcaggg caccgttccg cgacggccca aggtcgaggg
     2641 gaattcgtat gaagagaatt atggcactcc cattcgcatt cgcttttatg ttggcggcgg
     2701 ctccgctgtt cgccgcaagc cagtggtcca cggtcggcgg cgattcaggc aacacacgat
     2761 attcctcgct cacgcagatt aacgcgcaga atgtcacgaa attgggcgcg gcatggatgt
     2821 ccgaaaagat cacgccgcct ccatcgtctc gtggcgtacc ggtagttgat aaggatctga
     2881 tgttcctcac ggcgccgcca ttcgtttacg ccgtcaacat cggcactgga caaatcgcgt
     2941 ggaaatatcg cgcggcgccc ggtgagggag gcctgttcgg ccctccaggc tcgccggcgc
     3001 gagaaggcgt ggcggtgggc gaaggcctcg tcttcgtcgg gctttctgat tcgcatctga
     3061 tcgcgctccg cgaggatacg ggcgagcttg tatggaaaac gagcctcgct gcaccctctg
     3121 gcgagccaac cggggcgcca tcgggcgcgc ctttgtatgc cgatggactt gtctccatcg
     3181 gcacgaatgc ggattacggc taccgaggcc agattgtcgc cctcgatgcg aagaccgggc
     3241 acgaggcctg gcgctttttt gtggttccgt cgcccggcga acccggatcg aacacctggc
     3301 cgaaaaataa caatgcgtgg aagcacggcg gcggcgctat ttggctcgtc ggcacatacg
     3361 atccggcgct cggcctggtg ttctatgcca cgggaaatgc cgtgccccaa tacggcggtg
     3421 aacatcggcc gggcgacaac ttgtacaccg attgtgtggt cgcgcttgat agcaagaccg
     3481 gcaagcttcg ctggcattat caggtggttc accacgacat ctgggaagca gacgttgccg
     3541 aagcgcccgt gctgtttgac gcgcaggtga acgggcaatc tcgcaaggct gttgcggcca
     3601 tgcgcaccga tggctatctc ttcatgctcg atcgcgtcac tggaaagccg ctgggcaaaa
     3661 ttgaggagcg ccgcgtgcag caggacgcat ttcagaagac cgcggcaacg cagccctatc
     3721 ccgtcggcgc cgatcatgtt ctgccggatt gcgactactg gaaaacgcag cccctgccta
     3781 ggggtttcga gttgggttgc ttcttcacgc ctgccgcggc cgagaaaccg aacattctgg
     3841 ctccggttta tggcatgcga gcgacgccca tggcctttga tccgcagacg cagtttttct
     3901 atgtcaccgg cgatgccgcg ttgcaatggt tccggcgcgc cgacgaccca gattacttca
     3961 gcctaagttt cagccagcgc gtcccgggac tgaacaagct gggctacggc gtccttgccg
     4021 cgatcgacac tcgtacggac aagatcgcct ggaagaagga atttcatgat gagcggccga
     4081 gcggcgcgct tgccacagcg ggaggcctcg tttttcagat gcccggcgac ggcaatctgc
     4141 aggcctacga cgcaaccaat ggaaatctcg tatggcaatt tcagaccggt gcgcttggcg
     4201 gtagcccgcc gttcgcgttt gaaagcggcg gcgagcagta cgtcgccaca attgtcggcg
     4261 gcaacgtgtg ggcgtttaaa ttgggtggaa cgcttcccgc gcgtccagcg cctcctcttc
     4321 cgcctcagcc agagtttgcc ggaccgatca ccgacacagc aacaatccaa accacggcac
     4381 ttgcccgaga tatgggcctc acaggttcgc attacctcgg cgacgaatac gcgttcgatc
     4441 catatcgcgc gcgcgtcaag gccggcacgg aggttcgatg gcgaaacaat ggcgcggtca
     4501 ctcacaccat cgttgccgtt gatggctcgt ggacgaccgg ccctctgcag cctctggata
     4561 tcggcatcgt tagattcgac aagcccggca cgtacaccta catctgcaaa gatcatccat
     4621 gggtttacgg ccaaatcatc gtcgaacccg ccgctcccga agagcagcaa caaagtaaat
     4681 tgtcgggcaa agtagaaccg tagccctttc ccga
//
LOCUS       DATNDT010000519         3775 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3911758, whole genome shotgun sequence.
ACCESSION   DATNDT010000519 DATNDT010000000
VERSION     DATNDT010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3775)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3775)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3775
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3911758"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            573..2798
                     /gene="purL"
                     /locus_tag="VN788_16870"
     CDS             573..2798
                     /gene="purL"
                     /locus_tag="VN788_16870"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HXU22253.1"
                     /translation="MSAITPDVVAVHQITPSEYDKIISLLGREPTITELGIFSVMWSE
                     HCSYKSSRLHLKKLPTRSKLVLQGPGENAGIIDIGDGYAIAFKIESHNHPSFIEPFQG
                     AATGVGGILRDIFTMGARPIAVMDALRFGPLDVPRNRRILEGVVSGIAHYGNCFGVPT
                     IGGECVFEACYNGNPLVNVFALGVFKHDEIFYGKATGIGNPVIYVGAKTGRDGVHGAT
                     MASAEFREESKQKRPNVQVGDPFMEKLLLEACLEAMKTGAIVGIQDMGAAGLTCSTCE
                     MGSRANTGIDFDLERVPQRETGMTPYETMLSESQERMLLVAEKGREEEVFRVFRKWGL
                     DAVTIGKVTADGMLRVRDHGEVVAEISNRALADEAPVYDRPHTKPYRLAPLEGPAIPE
                     RTPAELQSDLIALLSSGDICSKRWIWEQYDYSVRTNTLEGPGADVAVVRIKETGTSIA
                     MALDGNARFCALDPREGTKLIVAECCRNLSVVGALPVAATNNLNFGNPERPEIMAQLV
                     ESIEGLAEACAFFETPITGGNVSLYNETLGEAIFPSPVIGIVGLMKTAKPTPIHFQNP
                     DRAIVLLGGLGESDAQRFGGTEYAKVILNSFWGLPPKLDMDYEKRIHQAIREIVAQGL
                     AESAHDLSDGGFAVALAECCFGPGEVGARVDLDEESNLAIALFHENPSRILISTADAQ
                     KIRQIAASHGVDATEIGVTMKEKLQINGHGVPLIDCEVRALRQPWQSSLEHLLRDSRV
                     A"
     gene            2795..>3775
                     /gene="purF"
                     /locus_tag="VN788_16875"
     CDS             2795..>3775
                     /gene="purF"
                     /locus_tag="VN788_16875"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HXU22254.1"
                     /translation="MNSQSSDKFHDECGVVAICGHPEAANLAYLGLHALQHRGQESAG
                     ISVSDGREIRTHKSMGHVVDIFTPEVVGSLPGEFAIGHTRYSTTGDTILCNAQPLSVN
                     CNKGSIAVAHNGNITNASELRHELEAEGSIFQASSDTEVVLHLVARSRERTLAGALRE
                     ALLQLEGAFSLVFLAEDRVIVARDPHGFRPLAFGYLDRGDGRRAWVFASETCAFDLIG
                     ATYVNDVEPGEMVITDRDGPVREHYTPERARAHCVFEHVYFSRPDSIVFGRPVQESRE
                     NLGRLLALSAPVDADVVVPVPDSGVTAAIGYSAESGIPFRQALIRNHYVGR"
BASE COUNT          796 a         1086 c         1104 g          789 t
ORIGIN      
        1 tgatcggccg gcagatcacc agggcatccg atcccaaggc cgaggcacag cgggttctcg
       61 aagaactagg catttcgtaa atcctcaaac aaatgggtac ggcgttgagc aagtttttcc
      121 gccaccgcat gagtcacgtc ccccgttctt gcccccgacc aaatgagtgc ggacgattaa
      181 ggcccgttga agaagaagtg tagtgtcggc ctggatagct gtgtagagaa tgcgcttcgc
      241 gatccggtgc accttttcga tgacatcttg ctgacgatta tctaaacagc caacgcgtag
      301 tatcgagggc gcgatttctc aagacacggt gcggctcatc cccggagtcg cgacgagcga
      361 cgattgcggc ctcctttccc tcaacgaaaa gcaagtcatc cgaaatggta gggagatttg
      421 caaacttgga gcagaagaag cccgacttag gccagaccgc caccacgatc aaccaacatt
      481 tagcgcacta cccgatctcc gagtaggccg tttgttaaaa taaaaattca catgagaaga
      541 cttgtctgcc gggcggttcg cacgaattcc cgatgagtgc cattaccccg gacgtggtcg
      601 ccgtccatca gatcacgcca tccgagtacg acaagatcat ttctctgctg ggccgcgagc
      661 ctaccattac ggaactcggc atcttcagcg tcatgtggag cgagcattgc tcgtataaaa
      721 gctcgcgtct acatctgaag aagctgccga cccgaagcaa gctggtgctg caaggtcccg
      781 gcgagaacgc gggcatcatc gatattggcg acggttacgc gatcgcgttc aagatcgaat
      841 cgcacaatca tccgagtttc atcgagccgt ttcagggagc ggccaccggt gtcggcggca
      901 tcttgcggga tattttcacg atgggcgcgc ggcccatcgc ggtgatggat gcgttgcgat
      961 ttgggccgct cgatgtcccg cgcaaccggc gtattctcga aggcgtcgtc tccggcattg
     1021 cccattacgg taattgtttc ggcgtgccga cgattggcgg cgagtgtgtc ttcgaggctt
     1081 gctacaacgg caatccactg gtgaatgtgt tcgcgcttgg cgttttcaag cacgacgaaa
     1141 tcttttacgg taaggccacc ggcataggca acccggtcat ttacgtgggc gcgaagacgg
     1201 gacgtgacgg tgtgcacggc gcaaccatgg cttccgcgga atttagggag gaatcgaagc
     1261 agaagcgccc gaatgtgcag gtcggcgatc catttatgga gaagctgctg ctcgaagcct
     1321 gtctcgaagc catgaagacc ggcgcgattg tcggtattca ggacatgggc gcggcgggtt
     1381 tgacctgctc cacttgcgag atgggcagcc gcgcgaacac cggaattgat ttcgatctcg
     1441 agcgtgtacc acagcgcgaa accggcatga caccgtatga aacaatgctt tccgaatcgc
     1501 aggagcgaat gctactggtg gccgaaaaag gccgcgaaga ggaagttttt cgcgtctttc
     1561 gaaaatgggg attggatgcc gttaccatcg gcaaggtcac cgcggatggc atgttgcgcg
     1621 tgcgcgatca cggggaagtc gtcgcggaaa tttcgaaccg agcgctggcg gatgaagcgc
     1681 ctgtttacga ccgtcctcac acgaagccat accggctcgc gcctctcgaa ggacccgcga
     1741 tacccgagcg tacacccgct gagttgcaaa gcgatctgat cgcgcttctc tcctccggcg
     1801 atatctgctc caagcgctgg atctgggagc agtacgacta ctcggtgcgc acgaacacgc
     1861 tggaaggacc gggcgccgac gtcgctgtcg ttcgtatcaa ggaaacggga acgtcgatcg
     1921 cgatggcgct cgatggcaat gcacgcttct gcgctctcga tccgcgcgaa ggtacgaaat
     1981 taattgtggc cgagtgctgc cgcaatctca gcgtggttgg cgctctacct gtagccgcca
     2041 cgaacaacct caattttggc aacccggagc ggccggagat tatggcgcaa ctggtcgagt
     2101 cgattgaagg gctggccgaa gcctgcgctt tttttgagac gcctatcacc ggtggcaacg
     2161 ttagcctcta caacgagacg ctaggcgaag cgatctttcc atcgcccgtg atcggaatcg
     2221 tgggcctgat gaaaaccgcg aagcccacgc cgatccactt tcagaacccg gaccgcgcca
     2281 tcgttctgct tggcggcctc ggcgaaagcg atgctcagcg cttcgggggc accgaatacg
     2341 ccaaggtaat tctgaattca ttttggggac tcccaccgaa gctcgacatg gattacgaga
     2401 agcgcatcca ccaggctatt cgcgaaatcg tcgcgcaagg gctggccgaa tccgcgcatg
     2461 acctgagcga tggcggtttc gccgtggcgc tcgccgaatg ctgtttcgga ccgggcgaag
     2521 tcggagctcg cgtcgatcta gatgaggagt caaaccttgc gatcgcgttg ttccacgaga
     2581 acccgtcgcg catcctgatt tccaccgccg atgcgcaaaa aattcggcaa attgccgcct
     2641 cgcacggcgt cgatgcgact gaaattggtg ttaccatgaa agagaagtta caaatcaatg
     2701 gccatggcgt gccgctcatc gattgcgaag ttcgggccct gcgtcagccg tggcaatcgt
     2761 ctcttgaaca cctcctccgg gattctcgag ttgcgtgaac tctcaaagct ccgataaatt
     2821 ccacgatgaa tgcggcgtgg tggctatttg cggccatccc gaagccgcga atttagctta
     2881 tctcggcctt catgcgctcc agcatcgcgg ccaggagagc gccggcatct ccgtaagcga
     2941 cggacgcgaa atccgcactc acaaatcgat gggacacgtg gtggacatct tcaccccgga
     3001 ggtggtcggc agcctgcccg gcgagttcgc gatcggccac acgcgttatt cgacaaccgg
     3061 cgacaccatc ctctgtaatg ctcaaccatt gtctgtgaat tgcaataagg gcagcatcgc
     3121 ggtcgctcac aacggcaaca tcacgaacgc atctgaattg cgccacgagc tcgaagcgga
     3181 aggctcaatt ttccaggcgt cgagcgacac ggaagttgtt ttgcatcttg tggcacgttc
     3241 ccgcgaacgg acattggcag gcgctttacg cgaagcgttg cttcagttgg aaggggcgtt
     3301 ttcgcttgta tttctggccg aagaccgcgt cattgtggcc cgcgatcccc acggcttccg
     3361 tccactggct tttggatatc tggatcgcgg cgatggtcgc cgcgcctggg tattcgcatc
     3421 ggaaacgtgc gctttcgacc tgatcggcgc tacgtatgtc aacgatgtcg agcctggcga
     3481 aatggtgatc accgatcggg acggcccggt tcgcgaacac tatacgccgg agcgcgcccg
     3541 tgcccactgc gttttcgagc acgtgtattt ttcccgtccg gattcgatcg tatttggccg
     3601 gccagtacaa gaatcgcgcg aaaatcttgg ccgcctgctt gcgctctccg cgcccgtcga
     3661 tgcggacgtt gtggttccag tcccggactc aggcgtcacg gctgcgatcg gctactccgc
     3721 cgaatccgga atcccgtttc gccaggcgct gatccgaaat cattacgtgg gccgc
//
LOCUS       DATNDT010000520         5599 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1765410, whole genome shotgun sequence.
ACCESSION   DATNDT010000520 DATNDT010000000
VERSION     DATNDT010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5599
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1765410"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1008)
                     /locus_tag="VN788_16880"
     CDS             complement(166..1008)
                     /locus_tag="VN788_16880"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HXU22255.1"
                     /translation="MQAILRNLTRAFGRLFPNCVILSQAIAFNMFLAFFPLLLLLLGV
                     LSGTPLFHDALSEIPTRLVTILPPGSSSVVVEYFVRQTAHPWRWIALGLGGTIIAGIQ
                     VMIGYMEGFRVIEGDVIKPGYWRRQLRALLLLCVTIVPMMIVMILTVFAKQVSTWRFF
                     QTGSPRLTRDIDWTCYALAIFVLAMIVLVSLYRLGRPGHASYRVLLPGAGVSTVLWWV
                     TDLSFGWYVRKMPYDVIYRGLASAIGLLVWMFLTAVIVLLGAAYNAEVREASAPKKLL
                     LFVP"
     gene            1132..2448
                     /locus_tag="VN788_16885"
     CDS             1132..2448
                     /locus_tag="VN788_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HXU22256.1"
                     /translation="MPEHKQTTSRSEEVIRKHQEYLWPAVTNYYRKPLVADHASMQYL
                     WDIEGNRYLDFFGGILTIGVGHCNPKVTSKITNQVDKLQHTSTLFPNEAIVALAEKLA
                     EITPGKLQKSFFTNSGTEANEAAILLARMAGPSYDVVALRHAYAGSSSLTKGVTGQAP
                     WRKSGVIGVGVAHALSPYCYRCPLNLKYPECGIACANDIENVIQTSTSGSIAAFIAEP
                     IQGVGGFITPPPEYFKIVFRIVKNYGGLFISDEVQTGFGRTGRKWFGIEQWEVTPDII
                     TCAKSMANGVPVGATITTAELADKFLGLTISTFGGNPVTSVAARAVIEVIEEEKLLEN
                     THTLGGYLRKKLEDLKEKYLLIGDVRGMGMMQGLELVRDRKTKEPAPTETTELMERAR
                     ANGLLIGKGGLHGNVIRLAPPMNISRSDVDEGIRLLDKSFNELPAG"
     gene            2595..3119
                     /locus_tag="VN788_16890"
     CDS             2595..3119
                     /locus_tag="VN788_16890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22257.1"
                     /translation="MELRSVRECRKIIIHPAMCLAAGMVALVLLQLFAFAGSMAAANG
                     DSNAIHWVSVSEAQVKLDDKTPLAWNVYQTEKKNKKDKKDSNLVLVLLGHRYLLLDTH
                     AKVVWEVPLTDLKAQGANFDSGNLTQQSRVIPSTDWSSRDVGPAEIIQLTLGDYGRVL
                     QVSLPHPPDLRPFY"
     gene            complement(3203..3730)
                     /locus_tag="VN788_16895"
     CDS             complement(3203..3730)
                     /locus_tag="VN788_16895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22258.1"
                     /translation="MSSVAAELSLRRRLLNRTAFFVLGSLAFVAVVVHYPPLDLDGVL
                     IFVGVIFFVTLGLAIWVERRALRHGDVEKLKRVYYGLVPLPWILSAILLSNGAMDRGP
                     WQIEQARVVGKFSMSGALPSRRLVVTSWRPDHRVERVPVDRRDFDLFNRGDVVNVTLG
                     TGLAGIPWVVGVSLH"
     gene            complement(3787..4065)
                     /locus_tag="VN788_16900"
     CDS             complement(3787..4065)
                     /locus_tag="VN788_16900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22259.1"
                     /translation="MAKKSNQMRWIMALGALALFGVLIYSSFQQTRRRYEVCVAFKGA
                     THCATAAGSSYEEAVRSAQTIDCELLANGRDENMVCLGNPPASVRALK"
     gene            complement(4082..4867)
                     /locus_tag="VN788_16905"
     CDS             complement(4082..4867)
                     /locus_tag="VN788_16905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22260.1"
                     /translation="MQPESDTKIQHARSTSGDELNALLHESDERTLLAILENSNLQEQ
                     HVTVLLERLDLPVSVLSALAGDGKWASSEGIRLRLASHPRTPKRFALASVRQLFLFDL
                     VRLSLLPSASADVRRVAEEVILTRVPHIQTGEKLTLARRGPARVAGAILAEGHPQAIK
                     LALANPFLTESQVLKVLAKVGAPERVVQAIAEHPKWSCRYNVRAALARNARAPVSAVL
                     AFLPDLTQRDLRDICTMDGLVPAVREHIEQELARRAEAAAKKT"
     gene            5280..>5599
                     /locus_tag="VN788_16910"
     CDS             5280..>5599
                     /locus_tag="VN788_16910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22261.1"
                     /translation="MNLKFAKKGIAITVLGLFLGGAAFGASPPSANSPAVTTEENWRS
                     IQIASDKLEELQSVSEKLSLVGDSMHASMQSVQSAREAHAYRLNAAREHINNAGRLLA
                     ELQG"
BASE COUNT         1340 a         1551 c         1641 g         1067 t
ORIGIN      
        1 gccgcgaata acgaagcgga gcgggcgctc ctcaggacga tatcccgcgt agcattccac
       61 gcgaatttcg ctcatcgcgc ctccgcaggc tgggttcgcc gcaaaggaac agcaacggat
      121 ttagtttagc acctgggacg accaccgcaa tcttgctacg cgtgttcatg gaacgaagag
      181 caaaagcttt ttgggcgcgg aggcctcacg cacctcggca ttataggcgg cgccgagaag
      241 aacgatgacg gccgtcaaaa acatccaaac cagcagacca atggctgacg ccaagccccg
      301 atagatcacg tcgtacggca tctttcgcac ataccagccg aaggaaagat ctgttaccca
      361 ccagagcaca gtcgaaaccc ctgcgcctgg aaggaggacg cgatagctcg catgacctgg
      421 acgacctagc cgatagaggg aaactaaaac aatcatagcg agcacgaata tcgcgagtgc
      481 gtaacaggtc cagtcaatat cccgagtgag acgaggcgat cccgtttgga aaaagcgcca
      541 ggtgctgacc tgtttcgcga agacggtgag aatcatcacg atcatcatgg gcacaatcgt
      601 aacgcacaaa agaaggaggg ctcgcaactg gcgccgccag tagccgggct tgatgacgtc
      661 accttcgatt acgcggaaac cctccatata gccgatcatc acctggatgc ctgcgatgat
      721 cgtgccgccc aggccgagcg caatccatcg ccaaggatgc gcggtctgcc ggacaaaata
      781 ttcaactacc acgctcgagc tccctggcgg caagatcgtc acaaggcgcg tggggatttc
      841 gctgagcgcg tcgtgaaata atggagtccc gctgagcact ccgagaagca aaagcagcaa
      901 cggaaagaaa gccagaaaca tattaaaagc gatagcctgg ctgaggatca cgcaattggg
      961 gaaaagccgg ccaaaggcgc gcgtcagatt tctgagtatt gcttgcattg ctgttcggcg
     1021 gattatacgg gaaggggcgt aaattgcggc actgattcag ggtgcccgcg ggcgcgcatc
     1081 cttgtcggcg tccggggggc agagtaaact tccgtgaaaa ctcgaggttc catgcctgag
     1141 cataagcaaa ctacaagccg cagcgaggaa gtgattcgca agcatcagga gtatctctgg
     1201 ccggcggtta ccaactacta ccggaagccg ctggtggcgg accacgcgtc gatgcaatat
     1261 ttgtgggaca tcgaaggcaa ccggtatctc gattttttcg gcggcatcct gacaattggc
     1321 gtgggccact gcaatccgaa ggtcacgtcg aaaataacaa atcaggtgga taagctccag
     1381 cacacttcca cactttttcc caacgaagcg atcgtggcgc tggccgaaaa gcttgccgag
     1441 attacacccg ggaaactgca gaagagtttt ttcaccaact cgggtaccga ggctaatgag
     1501 gctgccattc tgttggcgcg catggctggg ccgagctacg acgtggtagc tctgcggcac
     1561 gcatatgcag gctcgtcatc gttgacgaag ggcgtgacgg ggcaggcgcc ctggcgtaaa
     1621 tccggcgtga tcggcgtggg cgtggcgcac gcgctgagtc cttactgcta ccgttgcccg
     1681 ctgaacctga aatatccgga atgcggcatt gcgtgcgcga acgacattga aaacgtgata
     1741 cagacgagca ccagcggctc aatcgccgcg tttatcgcgg agccgattca aggcgtgggc
     1801 ggatttatta cgccgccgcc cgagtatttc aagatcgtat ttcgcattgt gaagaattac
     1861 ggcggacttt ttatttccga tgaagtgcag accggcttcg gacgcacggg aagaaaatgg
     1921 tttggaatcg agcagtggga agtgacgccc gacattatta cgtgcgcgaa gagcatggcc
     1981 aacggtgttc cggttggggc cacgatcact acggcggaac tggcggataa attcctaggc
     2041 ttgacgattt cgacgttcgg cggaaacccg gtgacgtccg tcgcggcccg cgcagtgatc
     2101 gaagtgatag aagaggaaaa actgctcgaa aatacgcata ccctcggcgg ctacttgcga
     2161 aagaaattag aggatttgaa ggaaaagtat cttttgattg gcgatgttcg cggcatggga
     2221 atgatgcagg ggcttgaatt ggtccgcgac cgaaaaacca aggagcctgc accgacggaa
     2281 accacggaat tgatggaacg ggcgcgcgcg aatggcctcc tgatcggcaa gggcggcctt
     2341 cacggcaacg tgatccggct ggctccgccc atgaatattt cccgttcgga tgtcgacgaa
     2401 ggcatacggc tgctcgataa gtcgttcaac gaacttcctg caggttagct gatggcggat
     2461 tctgtcgatc gaaggagttc gcccggtcgc ggtaggcggg cacgtcgacc aagccaagca
     2521 tgttcaccgg gcacggcatg ccgtgcccct accaaaaggg ccatctacgc ttgctctgat
     2581 gtattctgct gttgatggaa cttcggagcg tccgcgagtg ccgcaagatc ataattcatc
     2641 cggcaatgtg tctcgccgca ggcatggtcg cgcttgtgtt gcttcaatta tttgctttcg
     2701 ccggctcaat ggcggcggcg aacggcgatt caaacgcgat ccattgggtg tcggtgtccg
     2761 aggctcaagt aaagctggac gacaaaacgc cgctggcatg gaacgtctat cagaccgaga
     2821 aaaaaaacaa gaaggataag aaggattcca acctagtgct ggtgctcctc gggcaccgat
     2881 atcttttgct ggacacccac gcgaaagttg tttgggaagt tccgctgacg gacctgaaag
     2941 cgcaaggcgc aaatttcgat agcggcaatc tcacccagca atctcgcgtg attccaagca
     3001 cagattggtc ttcccgggac gtggggcctg ccgaaattat ccaactgaca cttggggatt
     3061 acgggcgcgt gctgcaagtt tcgctgccgc atccgcccga tctgcgtccg ttttactgag
     3121 ctgtttcagc gcgaggaaat cgcgcgcaat tctagtgttg agcaccagct gcttcgaaga
     3181 cgcgcggcgc gaaggaaatg cgctagtgca gtgaaacgcc gactacccaa ggaatcccgg
     3241 ccaggcctgt ccccagcgtt acgttcacaa cgtcgccgcg attgaataga tcgaagtcgc
     3301 ggcgatcgac ggggacacgt tccacgcgat gatccggccg ccaggaggtg acaaccagac
     3361 gtcgggaagg cagtgcgccg gacatggaga acttgccaac gacgcgggcc tgttcaattt
     3421 gccacggccc acggtccatc gctccgttgc taagcaaaat ggcggacaag atccacggca
     3481 gcggtaccaa gccgtaataa acccgcttaa gcttttccac atcgccgtgg cgcaaggcgc
     3541 ggcgttcgac ccatatcgcc aaacccaggg ttacaaagaa gatcacgccg acgaaaatca
     3601 agacaccgtc gagatcgagg ggaggatagt ggacgacaac ggcaacaaac gcgagcgagc
     3661 cgagcacaaa aaatgcggtg cggttgagca gcctgcggcg caacgacaat tcagcggcca
     3721 cggagctcat gccgcaggac tcgcacacgt ccctatcccg atccgcgccg caattcaggc
     3781 aaggcgtcat ttgagcgcgc gcacactcgc gggaggattg ccgagacaaa ccatattttc
     3841 atcgcgaccg ttggcgagaa gctcgcagtc gatcgtctga gcgctgcgga cggcctcttc
     3901 gtagctcgaa ccggcggcag tggcgcagtg cgttgcaccc ttgaaagcta cgcacacctc
     3961 atatcgccgg cgcgtttgct ggaaggagga gtaaatgagg acgccgaaaa gagccagcgc
     4021 gccgagggcc atgatccaac gcatctgatt ggattttttc gccatgtctg ccaaaggtac
     4081 tttaagtttt tttcgcagcc gcttccgctc tccgcgccaa ctcctgttcg atgtgttccc
     4141 gcacggcggg cacgaggccg tccatcgtac aaatgtcccg caggtcgcgc tgggtgagat
     4201 cgggtaaaaa cgcgagcaca gccgaaaccg gcgcgcgggc attgcgagcc agggctgcgc
     4261 gaacattata ccggcaggac cattttgggt gctccgcaat cgcctggacc acgcgctcgg
     4321 gcgcgccaac tttcgccaga accttcagca cctggctctc agtgagaaac ggattcgcga
     4381 gagccagttt gatcgcctgc ggatggcctt ccgcaagaat ggcccctgcc acgcgcgcag
     4441 ggccgcggcg tgcgagtgtg agtttttctc ccgtctggat atgtgggacg cgcgtgagaa
     4501 ttacttcttc ggccacgcgt cgaacatcag ccgacgcgga cggcaacaaa ctcaagcgga
     4561 ccaaatcaaa aagaaatagc tgccgaacgg aggcgagcgc aaaacgcttc ggcgttcgcg
     4621 gatggctcgc caaacgcagc cggataccct cgctggatgc ccacttcccg tccccggcca
     4681 gggcgctcag cacgctcacc ggcaaatcga gtcgctcgag cagcacagtg acatgctgct
     4741 cctgcaaatt ggagttctcg agaatggcaa ggagagtccg ctcgtctgat tcgtggagaa
     4801 gagcattgag ttcatcgccg gaggtcgatc gcgcatgctg gattttggtg tcggattcag
     4861 gctgcatttc ctttgggcgc ggagcggctt cagcagccga ttctacacgt ccaagcggct
     4921 agtccaaaac ccaattgaaa tgtacgaggg tggcctgcca ttgagggaaa aggcggaaaa
     4981 ctcgcccggt tcacaacgcc cgctgcgagg gacaggcgct gcaaaccggg ccgagaccaa
     5041 attcaccagg cgatggtatc gctcctccgt gagcgcggcg caacgccttt tcccgggtgt
     5101 cgccaaagga tcaattgact caatgtttag agcggctttg aaacggtagt tagataaaac
     5161 ctaactattt agaagaaact atcttctttt cctaacgatg tccggatgct atccaattca
     5221 tgtggcggcg taaccgctct atgcgcgcca aggataaaaa aaacaacagg gaggaacata
     5281 tgaatctcaa attcgcaaag aaaggcattg ccattaccgt tctgggactg tttcttgggg
     5341 gtgcagcttt tggcgcttct ccgccctcgg caaacagtcc ggcggtgacg actgaggaaa
     5401 actggaggtc gattcagatc gcctccgata aactcgaaga gcttcagtca gttagcgaaa
     5461 aactcagcct tgtcggcgac tcgatgcatg catcgatgca atccgtccaa agcgctcgcg
     5521 aggctcacgc gtaccgactg aacgccgcgc gcgaacatat taataatgcc gggcgtttgc
     5581 tggctgaact gcagggcat
//
LOCUS       DATNDT010000521         7578 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_95932, whole genome shotgun sequence.
ACCESSION   DATNDT010000521 DATNDT010000000
VERSION     DATNDT010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7578
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95932"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VN788_16915"
     CDS             <1..351
                     /locus_tag="VN788_16915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22262.1"
                     /translation="RDFFSTILKNSSQSRRFHIVPSLSQYAVITELDAASAIWVAKIF
                     RGLIASRSSNRNDADSNPAGAGKSRAKADCSHPVIAAYAQVTGALRYEAPVGFAEGSA
                     ACAAHRALSLAAKP"
     gene            438..1247
                     /gene="folP"
                     /locus_tag="VN788_16920"
     CDS             438..1247
                     /gene="folP"
                     /locus_tag="VN788_16920"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HXU22263.1"
                     /translation="MGVLNVTPDSFSDGGLYFDADAAAERAIEIERAGADILDIGGES
                     TRPGSEGISAEEELRRLLPVLERLRGRLKIPISVDTSKSEVAEAAAEAGAEILNDVTA
                     LRADPRLAEIARRRKLPLILMHMRGDPRTMQKKPFARNVRRDVSASLRRAVASARRMG
                     VSKSQIVLDPGIGFGKSTAQNFELLARLDEIARLGFPLMVGTSRKGFIGKTLGGLPAS
                     ERGWGTAASVTAAILAGAHIVRVHDVREMTQVARVADAVLRPDSVRADGAT"
     gene            1244..2506
                     /locus_tag="VN788_16925"
     CDS             1244..2506
                     /locus_tag="VN788_16925"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU22264.1"
                     /translation="MSGNASAVISPEQRANSRNALIAGFLGWTLDAFDYFILVYVVGQ
                     VATDFHRSIAEVSFALTASLLTRPIGAILFGLLADRYGRRLPLMLDVIFYSIIEILSG
                     FAPSYRIFIILRLLYGIGMGGEWGVGASLVMEAVPVKWRGMLSGILQEGYALGNLLAA
                     IAFWTIFPHWGWRAMFFLGGLPALLTLFIRARVKESQAWKAVAVARTSWRDYFRLVKA
                     NWKRFAYLVALMAAMNFISHGTQDLYPTFLQRQRHFDTRTTAIISVISMVGAIVGGIV
                     VGQISDRYGRRRAMVGSVLLAIILIPLWVFVPTAALTAVGAFAIQFMVQGAWGVVPAH
                     LNELSPGALRGFFPGLAYQTGVLIAAGAPTIEALMSRHLSYGQAMGLFALTVCAIGAV
                     LIACGPEAHRVSFESTSPAPAEASIASP"
     gene            complement(2522..4717)
                     /locus_tag="VN788_16930"
     CDS             complement(2522..4717)
                     /locus_tag="VN788_16930"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HXU22265.1"
                     /translation="MRGRQSRGDSGDWASALHDFVETPAVPGYENQLAEQVRNDIASF
                     HPVMDNLGDVIVTVGSGAPHRLIVTPIDEPGFVVSDITNDGYLRLQRLPQSGLPPIFN
                     ELYSAQPVKVRVAGGRWMNGVVTGLSIHLQTGRTNPPKSGDLDDMYVDIGASSAVEAR
                     KAGVDILSPVAIDRNLEDLATKEMAGASVGDKFGAAALVELLRKMDPANVKGTLTIAF
                     AVQQRAGARGLQRILRAIRPDEMIYVGRLLSGGAVPGMKSVHRAPRREPGSGALLGLA
                     QTNDELSGLTSELKQLASANNVPLATDYSAGLIPASYLPLPDLPAKWAHLAIATAWSD
                     TPAETIDAKDMAALERLLQLYTQANMPLPQQAIPGRDASRENHQEVSGAPSTAELLRR
                     LTTTYGASNHEQPVREMIKRFLPPWAKPETDDAGNLILHLGTASAAAKAPKILVIAHM
                     DEIGFEVKSISNDGRLEAEPLGGMILSFYQGHPASVHTSDRDLQAIVELPNGWDEATF
                     KWPTDSENPIRIDVGARSPEEAAKLGVKVGDSITIPKKYRPLLGTRANARSFDDRVGD
                     TALVSAVWSLGSSLHDRDITFVWSTGEELGLVGAAALAKRLAAEGDEPGYVFAVDTLV
                     SSDSPLESKRFGDAQIGKGFTIRAVDGSNIVPSPLVDRLLHLARANQIPVQLGVTGGG
                     NDGAAFVPYGAVDIALGWPLRYSHSPAEVIDTRDVDALSRIVAIIAKSW"
     gene            5156..6115
                     /locus_tag="VN788_16935"
     CDS             5156..6115
                     /locus_tag="VN788_16935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU22266.1"
                     /translation="MSTPAIEIIGLTKDYPVGFWRKRMRRSLDNLTLQVEEGEIFGFL
                     GPNGAGKTTTLKLLMGLIFPTAGTAKIRGCSIDDVRMHRDIGYLPEQPYFYDYLTARE
                     LLDYYARFSNYGAPERRERVERFLDRVGLASAGNIQLRKYSKGMLQRVGIAQAILHDP
                     KVIFMDEPMSGLDPVGRREVRDIMLDLKKQGRTVFFSTHILSDAEMLCDRVGVLSGGK
                     LQGVGAPGDIVSIEVHSMEILFEVKGSHPLPGELEEKATAIGGRYRVEVAEAGLYPAL
                     AQLESCNARILSISPVRPSLEDYFLRLVGREETVAPEVEVSSR"
     gene            6112..6891
                     /locus_tag="VN788_16940"
     CDS             6112..6891
                     /locus_tag="VN788_16940"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU22267.1"
                     /translation="MSATTGPVAINTFREAVRDRVLYNLVFFALVMIGAAILVGQISI
                     GIERLVIVNLGLSAISLFGLVMAIFIGVGLLSKEIEKHTLYSLLAKPIRRWEFLVGKY
                     AGLLLTLVINTAFMTLALAAALLYVGRPLVRGDVSILIAIYFILLALGLVTALALFFS
                     SFSSPMLSTLFTLGIYVMGIFAKDIRDFGDFTHNPLMKAFARVVYYLVPNFHNFNAIA
                     AAAYGEHISLLLVAQNTLYTVFYVTLLLVASSAIFSSRDLK"
     gene            6888..>7578
                     /locus_tag="VN788_16945"
     CDS             6888..>7578
                     /locus_tag="VN788_16945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22268.1"
                     /translation="MSRPSQRLRWIAAACTPVLLVCVAALQAEIDMRTRTATQERDEL
                     LLSSPSAVRKISLGYDSLLADIYWTRAVQYYGSRVATDHPNFDLLWPLLNITTTLDPK
                     LLIAYRFGGVFLSETGRGGAGRTDLAIKLIKRGIAANPGQWILGSDLGFLYYWRMRDY
                     PASAAAYLDASKKPGAPLWVKIMAARVAQKGGSLDTSRMIWSQLYESTKNSLVKKKAE
                     EMLLGLKVQEDE"
BASE COUNT         1515 a         2004 c         2404 g         1655 t
ORIGIN      
        1 cgcgatttct tcagtacgat attgaagaac tcgtcccaat cccggcggtt ccacattgtg
       61 ccctctcttt cccagtacgc cgtaatcact gagttggatg ctgcctcggc gatttgggta
      121 gcaaaaattt ttcggggcct aatcgcatct cgttcatcga accggaacga tgcggattct
      181 aaccccgcag gggcaggaaa gtcgcgcgcg aaggcggact gtagccatcc agttatcgca
      241 gcttacgcac aagttacggg ggcgttgcgg tatgaagcgc ccgtaggctt cgctgagggc
      301 agcgctgcat gtgctgcgca tcgggcgtta tcgctggcgg caaaaccgtg attcaatagc
      361 ggtccgtgtt tcgacgcaca aagtttcggt tgaggttgcc gtcacgaacg ctgacacttg
      421 gcgggcgcac gctagtaatg ggcgtgttaa acgtcacgcc cgacagcttt tctgacggcg
      481 ggctgtactt cgacgctgat gcagcggcgg agcgggcgat cgaaatcgag cgcgcgggag
      541 cggatattct ggatattggg ggcgagtcca cgcggcccgg ttcggaaggc atttcggccg
      601 aggaggaatt gcggcgactt ttgccggtgt tggaaaggct gcgcgggcgg ctgaagatcc
      661 cgatttcggt ggatacgagc aaatcggaag tggccgaagc ggcggctgag gcgggcgccg
      721 agattctgaa tgacgtgacg gcgctgcgcg ccgatccgag actcgccgaa atcgcgcgga
      781 ggcgaaagtt gccgctgata ctgatgcata tgcgcggcga tccgcgcacg atgcagaaaa
      841 aaccgttcgc gcgaaatgta cggcgcgatg tgagcgcgag cttgcgccgc gccgtagcgt
      901 cggcgcgccg gatgggagtg tcgaaatcgc aaatcgtgct cgacccgggg attgggttcg
      961 ggaagagcac cgcgcagaat tttgaattgc tggcgaggct ggatgaaatc gcgcgcctgg
     1021 gttttcccct gatggtcggc acgtcgagga agggttttat cggcaaaacg ctcggcgggc
     1081 ttccggcgtc ggagcggggc tggggtacgg cagcaagcgt gacggcggcc atcttggcgg
     1141 gcgcacacat tgtgcgcgtc cacgacgtgc gcgaaatgac gcaggtggcg cgcgtggccg
     1201 acgcggtatt gcggccggac tcggttcgcg cggatggcgc gacatgagcg gcaacgcgtc
     1261 cgcggttatt tctcccgagc agcgcgccaa ctcgcgcaat gcgctgatcg ccgggtttct
     1321 cggttggacg ctggacgcct tcgactattt catcctggtg tacgttgtgg gccaagtggc
     1381 gacggatttt caccgctcga ttgcggaagt ttcgtttgcc cttacggcga gcctgctaac
     1441 ccggccgatc ggcgcgattt tgttcggctt gctggcagac cgttacggtc gccgcttgcc
     1501 actaatgctg gacgttatct tctactcgat cattgagatt ctttccggtt tcgcgccttc
     1561 ctaccgaata ttcataatat tacggctgct ctacggaatc ggcatgggcg gggagtgggg
     1621 cgtgggtgcg tcactggtga tggaagccgt gccagtgaaa tggcgaggaa tgctttccgg
     1681 gattttgcaa gaagggtacg cactggggaa cctgctggcg gcgatcgcgt tctggacgat
     1741 ttttccgcat tggggctggc gggcaatgtt ttttcttggg ggattgcccg cgctgctgac
     1801 cctctttatt cgcgcgaggg tgaaggaaag ccaggcgtgg aaggcagtgg ctgtcgcgcg
     1861 gacgagctgg cgcgactatt tccggctggt gaaggcgaac tggaagcgct ttgcgtacct
     1921 ggttgcgttg atggcggcga tgaatttcat ttcgcacggg acgcaggacc tctatcccac
     1981 ttttttgcag cgccaacgac acttcgacac gcgcaccacg gctatcatca gcgtgatttc
     2041 gatggtcggc gcaatcgtgg gcggtatcgt tgtggggcag atttcagacc gatatgggcg
     2101 acgccgcgcg atggttgggt cggtgctgct ggcgattatt cttataccgc tgtgggtgtt
     2161 tgtcccgacg gccgcgctga cagcggtagg agcattcgcg atacaattca tggtgcaggg
     2221 ggcgtggggc gtggttcccg cgcatcttaa tgagctttcg cctggcgcct tgcgcggatt
     2281 tttccccggg ttggcttacc agaccggcgt gctcatcgcg gcgggcgcgc cgacgattga
     2341 agcattgatg agccgtcatc tgagctatgg gcaggcaatg ggattatttg cgcttacggt
     2401 ttgcgcgatt ggagcggttt tgatcgcatg cggaccagaa gcgcaccgtg tatcgtttga
     2461 atcgacgagc cctgccccag cggaagcaag tattgcgtcc ccgtgacact tagcggagcg
     2521 gctaccaact ctttgcaatg atggcaacga tgcggctcag ggcgtccacg tcccgcgtat
     2581 ctatcacttc tgccggcgag tgggaatagc ggagcggcca gcccagggcg atgtccaccg
     2641 caccataggg gacgaatgcg gcgccgtcgt tgccacctcc agtaacgccc aattgcacag
     2701 gaatctgatt ggctcgcgcc aggtgaagga ggcgatcgac gagaggcgaa ggcacgatat
     2761 tggagccgtc gacggcgcga atggtgaagc cttttccgat ctgcgcatcg ccgaagcgtt
     2821 ttgattcgag cggcgaatcc gagctgacca aggtgtcgac ggcaaagaca tagccgggtt
     2881 catcgccttc ggcagctagg cgtttcgcca gcgcggcggc gcccacgaga cccaattcct
     2941 cgccagtgga ccagacgaag gtgatgtcgc ggtcatgcag cgaagagccg agggaccaca
     3001 cggcggagac cagcgcggtg tcgcctacgc ggtcgtcgaa gctgcgggcg ttcgcacgcg
     3061 tgccgagcag cgggcgatat ttcttgggaa tggtgattga gtcgccgacc ttgacgccga
     3121 gcttcgccgc ttcttcaggg ctgcgcgcgc ctacatctat gcggatcgga ttctccgagt
     3181 cagtcggcca cttgaaggtg gcttcatccc agccgtttgg caattcgacg atggcttgga
     3241 gatcgcgatc cgatgtatga accgacgccg ggtggccctg ataaaagctc aagatcatgc
     3301 cgccgagagg ctcagcttcg agcctgccgt cattggagat cgatttgacc tcgaagccga
     3361 tctcgtccat gtgcgcgatc acgagaatct tgggtgcttt cgcagcggcg gatgctgtgc
     3421 cgaggtgcaa gatcaggttt ccagcgtcat ctgtttccgg tttggcccac ggcggaagga
     3481 aacgctttat catctcgcgt acgggctgtt cgtggttgct cgcaccgtat gtggtagtga
     3541 ggcgcctcag cagctcagcg gttgagggcg cacctgagac ttcttgatgg ttctcgcggg
     3601 aggcgtcgcg gccggggatg gcttgttgtg gcagcggcat gtttgcctgg gtgtaaagct
     3661 ggagcaggcg ctcgagggca gccatgtctt tcgcgtcaat ggtttcggcg ggtgtatcag
     3721 accaagccgt ggcaatagcc aggtgcgccc actttgccgg taagtccggc aaaggaagat
     3781 agctggcggg aataagaccg gcggaatagt cggtggctag cggcacatta ttggctgagg
     3841 cgagttgctt gagttcggaa gtgaggccag agagttcgtc attggtttgc gcgaggccga
     3901 gaagggctcc gctgccgggc tcgcggcgcg gggcgcgatg cacgctcttc atcccgggaa
     3961 ccgcgcctcc ggaaagcaag cgacccacat agatcatttc gtccggccga atcgcgcgca
     4021 gaatgcgctg caggccccgg gcaccggccc gttgctggac ggcaaaagca attgtcaggg
     4081 tacctttcac gttcgcagga tccattttgc gaaggagttc aaccagggcg gcggccccga
     4141 acttatcgcc tacggaggca ccagccatct ctttggtggc aagatcctcc aagtttcggt
     4201 cgatcgcaac cgggctgaga atatccacac cggccttgcg cgcctcgacg gcggagctgg
     4261 cgccgatgtc gacatacata tcgtcgagat cacccgattt cggcggattt gtgcggccgg
     4321 tttgcaaatg gatagagagg ccagtgacaa ctccgttcat ccatctgccg cccgccactc
     4381 ggaccttcac cggctgcgcg gaatacaact cattgaagat tggaggtagc ccggactggg
     4441 gcaaccgttg caagcgcagg tagccatcgt tcgtaatgtc gctgacgacg aagcccggtt
     4501 cgtcgatcgg cgttacgatc agacggtggg gtgcaccgct cccgacggtg acgatcacgt
     4561 cgcccagatt gtccatgact ggatgaaagg aggcgatatc gtttcggact tgttcggcaa
     4621 gctggttctc gtatccaggg acggccggag tttcgacaaa atcgtggaga gcgctggccc
     4681 agtcgccgga atcgcctcgc gattgccgtc ctctcacagc gctttgaccg ctcgccggag
     4741 atatgcagaa aattgaagat accaatagaa acaagaggat gtccaggagc cgagaacttg
     4801 acgtgttcga cacttcgaaa aagcgacggg tagaagaatc tggagtgctt tgtttagaat
     4861 tgatcacggg cgatgccatt cgacgtcgag cggcgatcgg ctgctgcaaa gcgacataat
     4921 tcgaagtggc cgcattgtcc ttaccggcgc cagttttggc taccctcaat ctggatccta
     4981 aaggcatcat agattcgacc aaagtagcac gatgcgatga gcttgtctgg aacttattga
     5041 cgtaaggaac ggccattgcg tcaaagaaaa atcgcgggat cgagagcacc gctttaggta
     5101 acgtcgtcga attgacatga gaccgaagtg gaaaaaataa ctggaaaaac atgcgatgag
     5161 cacaccggcc attgaaatta tcggtttgac aaaagactac ccagtcggct tctggcggaa
     5221 gcggatgcgg cgctcgctcg ataacctaac cctccaagta gaagagggtg aaatttttgg
     5281 cttcctggga ccgaacggcg cgggaaaaac gacgacgctt aagcttctga tggggctgat
     5341 ctttccgacc gctggaacgg cgaaaattcg aggctgttca attgacgatg tacgcatgca
     5401 tcgggatatc ggctacctcc cggaacagcc ctatttttac gattacctaa cggcgcgcga
     5461 gctcttggat tactatgcgc gattttcgaa ttacggcgcc cccgaacgcc gcgagcgcgt
     5521 ggagcgtttt ctcgaccggg taggattggc gtccgccggc aacatacagc ttcgaaagta
     5581 ttccaaaggc atgcttcaac gggttgggat cgcgcaagcc atcctgcacg acccgaaagt
     5641 gatttttatg gatgagccga tgtccgggct agacccggtg ggccgtcgtg aggtgcggga
     5701 catcatgctc gatctgaaaa aacaggggcg aacggttttc ttttcgactc acatactttc
     5761 cgatgccgaa atgctctgcg accgcgtggg agtgctctcg gggggcaaat tgcagggagt
     5821 tggcgcacca ggcgacatcg tgtcaatcga agtgcactcc atggaaattc ttttcgaggt
     5881 aaaggggagc cacccgttgc cgggcgaact cgaggaaaag gcaacggcaa tcggcggacg
     5941 ttacagggtg gaagtggcgg aggccggact ctatccagca ctcgcacaac tcgagagctg
     6001 caatgcacga atactttcga tctctcccgt gcggccgagc ctggaagatt atttccttcg
     6061 gctggtcggt cgcgaagaaa ctgtggctcc agaagtggag gtgagttccc gatgagcgcg
     6121 actacaggcc ctgtggcgat caatacgttt cgcgaagcgg tgcgcgaccg ggtgctgtac
     6181 aacctggtat ttttcgcctt ggtgatgatt ggcgcggcca ttcttgtcgg acagatctcg
     6241 attggcattg agcggctggt gatcgtgaat ttgggtcttt cagcgatttc tcttttcggg
     6301 ctagtaatgg cgattttcat aggcgtcggg ctgctgtcca aggaaatcga gaagcacact
     6361 ctctacagcc tgctggcaaa gcctatccgc cgctgggaat tcctggtggg gaaatatgcg
     6421 ggcctgctgc tgacgttggt gataaacacc gcgttcatga cattggctct agcggctgcg
     6481 ttactttatg tcggtcgacc actcgtgcgg ggcgacgtat ccattctcat tgcgatctac
     6541 ttcatactgc tcgcgctcgg gctggtaacc gccctggcac tcttcttctc gtccttctcc
     6601 agcccgatgc tttcgaccct atttacgctg ggaatctacg taatgggaat atttgccaag
     6661 gacattcgcg acttcggcga tttcacccac aacccgttga tgaaggcgtt cgcacgggtg
     6721 gtctattatt tggttccgaa ctttcacaat ttcaacgcga ttgcggctgc cgcatacggc
     6781 gagcacattt cattgttact agtggcccag aacacgctgt acacagtttt ttatgtgacg
     6841 ctgcttttgg tggcgtcgtc tgctattttt tcgagccgcg acctaaaatg agccgtccga
     6901 gccaaagact aaggtggatc gccgctgctt gcacgccggt gctactcgtc tgcgtggcgg
     6961 cgttgcaagc ggaaattgat atgcgtacgc gcacggcgac gcaggagaga gacgaactgc
     7021 ttctctcatc gccctccgcc gtgaggaaga tcagtctggg atacgactcg ctgctggcgg
     7081 atatatattg gacgcgcgcc gtgcagtact atggcagcag agtggcaacg gaccacccga
     7141 atttcgattt gctgtggccg ctgctcaata ttacgaccac gctggatccc aagctgctga
     7201 ttgcttaccg cttcggtggt gtgttccttt cggaaaccgg cagaggagga gcaggacgaa
     7261 ccgatctggc tatcaagctt ataaagcgtg gaatcgccgc aaatccaggc cagtggatac
     7321 tcggctcaga tctgggtttc ctctattact ggcggatgag agactacccg gcttccgcgg
     7381 cggcgtatct cgacgccagc aaaaagccgg gcgcgccgct ttgggtaaag ataatggctg
     7441 cccgtgtagc ccagaagggc ggatcgctgg atacatcgcg catgatctgg tcgcaattgt
     7501 atgaatccac taaaaattcg ctggttaaaa agaaggccga ggaaatgctg ctggggttga
     7561 aggtgcagga agatgaaa
//
LOCUS       DATNDT010000522         7440 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1615736, whole genome shotgun sequence.
ACCESSION   DATNDT010000522 DATNDT010000000
VERSION     DATNDT010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7440
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1615736"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1245
                     /locus_tag="VN788_16950"
     CDS             <1..1245
                     /locus_tag="VN788_16950"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch repair protein"
                     /protein_id="HXU22269.1"
                     /translation="DNLRVRLNPESLIGWAEAGSVLPRHGLRLVAIALSLCIVGALIY
                     WAGTGDYLPLLIGVIIAAIVNRSFHNRIKEILGEFPANADGVLLFARILERIEAETFT
                     DPRLQKLSAELKRGGTTASSAIRKLARLAFWIDAHHSLIGAILEWLVLYSLQLAAAAE
                     AWRRKYGRRMRACVEITAEMEALLSLAAYSHEHPADPFPEIVETPDRPALFEGKQLGH
                     PLIPVARCIRNSVTLNADTQVLLVSGSNMSGKSTLMRTVGINAVLAFAGAPIRGKALR
                     LTPLSLGTRIRTVDSLQEGLSNFYAEILRIRKVFELLGGERPVLFLFDELLEGTNSHD
                     RRIGAESLVRALIDDGAIGIVTTHDLALTDMAASMRSAVRNAHFEDYIENGEMRFDFK
                     LREGVVTKSNALELMRLVGLKV"
     gene            1693..2382
                     /locus_tag="VN788_16955"
     CDS             1693..2382
                     /locus_tag="VN788_16955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22270.1"
                     /translation="MFGDFTIDRRESLPPEGPAGANPVVAAMASPESLAGNEPNLEDR
                     VVIEVDTVNPTPMTITIAEAIVFLLTNANRFAEPEPAPMCRRRRQVPTSQTPAPSQPE
                     PALSHHARHCTICHHPERQAIDEEFTHWYSPKTIASDYAVGMRSVYRHARALGLFERR
                     AANLREALGHIIEKVENARSLPTPSTRFTPMRTYRLPGDGLNRRSSSPLISGAIGANR
                     HTGRVEHDATY"
     gene            complement(2453..3952)
                     /locus_tag="VN788_16960"
     CDS             complement(2453..3952)
                     /locus_tag="VN788_16960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HXU22271.1"
                     /translation="MATAVAPTVFKNYINGEWTEARSGKAFEDRNPANTGELVGMFPA
                     SAEEDVAAAIEAAANAYRHWRLTPAPRRAEILFRAAEILVARKEDYARDMTREMGKVL
                     DETRGDVQEAIDMTYLMAGEGRRQFGHVAPSELPNKFAMAERVPVGVAGLITPWNFPM
                     AIPSWKAMPALVCGNTVVLKPAEDTPLSSYHLAKALEEAGLPAGVLNVVFGDGPSAGA
                     PLLAHPGVGLVSFTGSTETGRIVSEACAPTFKKCHLEMGGKNIIIVMDDANLELAIEG
                     AVWGGFGTSGQRCTAASRIAVQKGVYHKFVQKFVERVKALKVGDGLDATTQMGPCINE
                     QQLKTVMSYVEIGKAEGAKLATGGHRLETGPHARGWFHEPTVFTDCDPMMRICQEEIF
                     GPVVCVIPFDDFEDALEIANDVKYGLSAAIYTRNVNESFQALRDLETGIVYVNAPTIG
                     AETHLPFGGTKNTGNGHREAGLAALDFYSEWKTLYIDYSDRLQRAQIDH"
     gene            complement(4100..4663)
                     /locus_tag="VN788_16965"
     CDS             complement(4100..4663)
                     /locus_tag="VN788_16965"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HXU22272.1"
                     /translation="MKSLIVAVASLALGCGAFPTVAAARPRGPGSPATASATPVHHKR
                     HHTAKRRRHVKRVRGQKAPTADRITEIQTALSRDGYYQGEPNGKWDSNTVAAVQKFQS
                     ANGMDANGKLDAPTLQKLGLGSDIAGVSAPKPIVHGSPNAAPSLSAPGAPPAPRTSTS
                     SSGGPVASVNSSSPSASQNPPAPTSNH"
     gene            complement(4874..6421)
                     /locus_tag="VN788_16970"
     CDS             complement(4874..6421)
                     /locus_tag="VN788_16970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HXU22273.1"
                     /translation="MGIQFRELWSWVRRRKIFASLLVALTLCVGIVIGASFSGHALAN
                     RAQGANGASLLAVPDPVSLSGAFAKISKSLGPAVVNISTTQVIEKPKGGHAPHGQNDP
                     FQDFFDRFFNSPDNAPDAERSLGSGVLVDKRGFILTNDHVIDGATKIQVALDGDPGHY
                     NARVIGTDKETDLAVIKIDANHDLPVAKLGNSDGVEVGDWVLAFGSPFGLNSTVTAGI
                     VSAKDRSNVGHQFQRFIQTDAAINPGNSGGALVSLGGEVIGINTAIYTGSRGFEGVGF
                     ALPSNTAITVYNQLVTSGKVTRGSIGVSFQDENSNNPVLMKELGAPYGIVVEAVEPGS
                     PADKVGLQPGDVITDVNGQPVHNGTDLVNPIAQTPIGQSVKLHYVRNKQSKEVTVSVA
                     DRSKLFPQNAESDESQPDQSEEPGQFGVHAEELTPDLAHKLGMTKLSGAVITEVEPAS
                     FAEDVGFARGDVILEINHVPVNSFADYRAQMSKLKPGQDVLFKIARHQGDRVLTLFLA
                     GMVPASP"
     gene            complement(6468..7310)
                     /locus_tag="VN788_16975"
     CDS             complement(6468..7310)
                     /locus_tag="VN788_16975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HXU22274.1"
                     /translation="MRRRFFVENFSDAAAVLRGEAADHLGRVLRAQPGQFYELSDGHR
                     VWLGKIERVALAKRGKSRIEFALIETVPTSEPSLRIDLLISLVKFDRFEWCLEKATEL
                     GANEIVPLAAARTDKPLVAAAAKRHERWQKILRESAQQSRRLGPPVLHEIGTPEQAFR
                     KYSAGLRIVLSERSDAPPLRAVLAGTSESWLQPDSSSGHDFGRAASRADSERLQPLRE
                     GVSAALAIGPEGGWTAQEIEAARASGFAEASLGKNILRTETAVLASLSILAFALEPGD
                     APAE"
BASE COUNT         1551 a         2243 c         2101 g         1545 t
ORIGIN      
        1 gacaatttgc gtgtgcgttt gaatcccgaa tcgctgatcg gctgggcaga ggctggatca
       61 gttctgcctc ggcatggcct tcggcttgtg gccatcgctt tgtccttatg cattgtcggg
      121 gcgctcatat attgggcagg caccggcgac tatttgccgc tgctgatcgg cgtaatcatc
      181 gctgcaatcg taaatcgaag tttccacaac cgtatcaaag aaattctcgg ggaatttccc
      241 gcaaacgccg acggcgttct gttgtttgcc cgaattctcg aaagaatcga ggcggaaaca
      301 ttcaccgatc ctcgcttgca aaaactttcc gctgaactca agcgcggggg cacaaccgct
      361 tcgagcgcga ttcgcaaact ggcccggctc gccttctgga ttgacgcgca ccatagtctg
      421 atcggcgcaa tactcgagtg gcttgtgctt tactccctgc agctcgcggc tgccgccgag
      481 gcgtggcgcc ggaagtacgg ccgcaggatg cgagcctgcg tggaaatcac tgcggaaatg
      541 gaagcgctgc tttcgctcgc tgcatactcg catgagcatc ccgcggatcc ctttccggaa
      601 atcgtcgaaa cgccagaccg tccggcactt ttcgaaggta aacaactcgg gcacccgctg
      661 atacccgtcg cgcgatgcat ccgcaacagc gtcacgctga acgccgacac ccaagtactg
      721 ctagtgagcg gctcgaacat gtccggcaag agcactctca tgcgcacggt ggggatcaat
      781 gctgtactcg ccttcgccgg cgccccgatt cgaggaaaag cgctgcgcct cactccgctg
      841 tctctaggga cacgcatccg aaccgttgat tccctgcaag aagggctttc gaatttctat
      901 gcggagattc tgcgcatccg caaagtcttc gaattgctcg gtggcgaaag acccgtgctc
      961 tttttattcg atgagctgct tgaaggaacc aactcgcatg accgccgtat aggcgccgag
     1021 tccctggtgc gcgcgctgat cgacgatggc gcgattggaa tcgtcaccac gcacgatctg
     1081 gcgctcacgg acatggcggc ctccatgagg agcgccgtac gcaacgccca cttcgaggac
     1141 tacatcgaaa acggtgaaat gcgcttcgat ttcaagctcc gcgaaggagt cgtaaccaaa
     1201 agcaacgccc tcgaactaat gcgcctcgtc ggcctgaaag tctgacggtt ctcatgtagc
     1261 gccatccctt cagggcggca tcttggctgt atcgcaacca atgccaccgc cgcgccattc
     1321 cgcatgccct gacatgggaa gggagcggag cctgccggtt ttttttcttc cgttccgcgt
     1381 gtttttgcaa cggatgccgg caggcgcagt tcgaagcgcg ccttctgcgc cccgaatgta
     1441 gttacgggaa atttctcttg gatgtaaccc gcaccacgac caccgcagct gcgtcattcc
     1501 gagcgaagtg tcccgcgcct ttgggttttg cgcgaagcgc cgggacacgc agtcgaggaa
     1561 tctctcttcg gtgtaacgcg cggcaacatt tcatcattct ttacaccacg cactttctga
     1621 tttcccttgc gcaatctgtc aagctgtgaa tcgaggtcag agcttcgagt aacgcgaaag
     1681 gagatgcaag aaatgttcgg agactttaca atcgaccgcc gagaatcgct gccgcctgaa
     1741 ggcccggcag gagcaaatcc cgtcgtggcc gcgatggcct ctccagagtc gctagcgggg
     1801 aatgagccga acctggagga ccgggtcgta attgaagtcg ataccgtcaa ccccacccca
     1861 atgaccatca ctatcgccga agcgattgtg tttctgttga caaacgcaaa tcgcttcgcc
     1921 gagcccgagc cagcgccgat gtgccgtcgt cgcagacagg tccctacttc tcaaacaccc
     1981 gccccatcac agccggaacc tgcgctctcc catcacgcgc gacactgcac catctgtcat
     2041 cacccggagc gccaggccat cgacgaggaa ttcactcact ggtacagccc gaaaaccatt
     2101 gcctccgatt atgcggtcgg aatgcgctcc gtttaccgtc acgctcgcgc cctcggcctt
     2161 ttcgagcgcc gcgccgccaa cttgcgcgag gcgctcggac acatcatcga aaaggttgaa
     2221 aacgcccgat cacttccaac gccgtccacg cggtttacgc ccatgcgcac ctatcgcctt
     2281 ccgggcgatg gattgaaccg ccgctcgtcg tccccactaa tcagcggcgc catcggagct
     2341 aatcgacaca ccggtcgagt agaacatgat gcaacttact gaaaacacgg ctcggatggt
     2401 ccgcctaccc gtcacacttt tgagggagac ggaatcgcat ccggagagcc agctaatggt
     2461 cgatttgagc gcgttgcaga cggtcggaat agtcgatgta gagggtcttc cattcggagt
     2521 agaagtcgag cgcagcgaga ccggcttcgc ggtggccgtt gcccgtattc ttggtcccgc
     2581 caaatggcaa atgggtctcc gcgccgattg taggagcatt cacatagacg atcccggttt
     2641 caagatcgcg caaagcctgg aaggattcgt tcacattccg cgtgtaaata gcagcagaca
     2701 aaccgtactt tacgtcgttg gcgatctcca gagcatcttc gaaatcgtca aatggaatca
     2761 cgcaaacgac cggcccgaag atttcttcct ggcagatgcg catcatcgga tcacaatcgg
     2821 tgaagactgt cggttcatgg aaccagccgc gggcgtgcgg tccggtttcc aggcgatgtc
     2881 ctcctgtggc gagtttcgct ccttctgcct ttccaatttc gacgtagctc atcacggttt
     2941 tgagctgctg ctcgttgatg catggcccca tctgggttgt cgcgtcgagg ccgtcgccga
     3001 ctttcagcgc cttcacgcgc tcgacaaatt tctgcacgaa cttatgatac acgccctttt
     3061 gcaccgcgat gcggcttgcg gccgtacacc gctggccgct ggttccgaat ccgccccaca
     3121 ccgcgccttc aatcgccaat tcgaggttcg cgtcgtccat cacaatgatg atgttcttgc
     3181 cgcccatttc cagatggcac ttcttgaatg tcggcgcgca ggcctcgctc acgatgcggc
     3241 ccgtttcggt cgatcccgta aaactgacaa ggccaacgcc aggatgcgcg agaagcggcg
     3301 ctccggccga agggccgtcg ccgaaaacaa cattcagcac gccggcaggc aagcccgcct
     3361 cttccagcgc cttcgcgaga tggtaggacg aaagcggcgt atcttcggcg ggcttcaaca
     3421 ccaccgtatt tccgcaaacg agcgctggca tcgctttcca cgaaggaatc gccatcggaa
     3481 aattccatgg agtgatcaat cccgccactc ccacgggaac gcgctcagcc atcgcgaatt
     3541 tattcggcag ctcggaggga gctacgtggc cgaactggcg gcggccttcg ccagccatca
     3601 gatacgtcat atcaatggct tcttggacat cgccgcgcgt ttcgtcgaga actttgccca
     3661 tctcgcgcgt catgtcgcgc gcgtaatctt ccttgcgggc gacaagaatt tcggcggcgc
     3721 gaaaaagaat ttcggcgcgc ctgggagccg gcgtgagccg ccagtggcga tacgcattcg
     3781 ccgcggcctc gatggccgca gcgacatctt cttccgccga tgcgggaaac atgccgacca
     3841 actcaccggt attcgcggga tttcggtcct cgaaggcctt gccgctgcga gcctcggtcc
     3901 attcgccatt gatgtaattc ttaaaaacag tgggggcaac cgcggtggcc attgtcattc
     3961 ctccagtact gccgttgcgc gcgggcagtg cacaaagcct cggctaagct gtaacgcaaa
     4021 actacggcac acgcagaact ttagcctagc aaccgcgagg tttcggcgtc aaatgcgcgt
     4081 gggtccatgc ttcccactac taatgattcg atgtaggcgc cggaggattc tgactagcgg
     4141 agggagacga agaattaacc gaagctaccg gcccgccgct ggacgacgtc gatgtccttg
     4201 gcgcgggtgg cgcgcccggc gcgcttagcg aaggagccgc attcggcgaa ccatggacaa
     4261 tcggcttcgg cgcggaaacg ccggcaatgt ccgatcctag gccgagcttt tgcaaggttg
     4321 gcgcgtcgag tttgccattc gcgtccatgc cattagcgga ttgaaatttc tgaactgctg
     4381 caaccgtgtt ggagtcccat ttgccatttg gttcgccctg gtaataaccg tcgcgggaga
     4441 gagcggtctg gatttcggta atgcgatcgg cggtgggagc cttttgtccg cgcaccctct
     4501 taacatgacg gcgcctcttc gctgtatggt gccgtttgtg gtgaacggga gtcgcggaag
     4561 ccgtggcagg cgaaccgggc ccgcgcgggc gagccgcggc gacggtagga aaggcaccgc
     4621 atcccagcgc caaggatgca actgcaacga tgagggattt catacgattg ctctcccgac
     4681 cgatctacgg ctggtccaga actgagattc ccggcacagt ttgcgggtaa tcccgattat
     4741 gcaggtttag cgtcgcagat taaagccggg cctcccgtga acggacctat caagcaacgg
     4801 cccaaaaacc cgtactcgtc ttatttgacg aagctgcatg ccacggcgca gccctggcaa
     4861 tttcgatatg tggctatgga gatgccggaa ccatgccggc gaggaacaag gttaggacac
     4921 ggtcgccctg atgacgcgcg atcttgaaga gaacatcctg acccggcttt agcttcgaca
     4981 tttgcgcgcg ataatcggcg aatgaattta ccggcacgtg attgatctcg aggattacgt
     5041 cgccacgcgc gaaaccgaca tcttcggcaa agctggcggg ctcaacctca gtgattacgg
     5101 cgccgctaag cttggtcatg ccgagcttat gggccaagtc cggagtaagc tcctcggcgt
     5161 gtacaccgaa ttgcccgggt tcttcgcttt ggtccggttg cgattcatcg ctttcggcat
     5221 tctgcgggaa aagctttgaa cgatccgcga cgctaacggt gacttctttg ctctgtttgt
     5281 tgcgcacgta gtggagcttg accgattggc cgataggagt ttgcgcgatg gggttaacaa
     5341 gatcagtgcc gttatgaact ggctggccgt ttacgtcggt aatgacgtcg cctggttgca
     5401 gcccgacttt atccgcgggg ctgcccggct caaccgcctc gaccacgatg ccatacggcg
     5461 cacccagctc cttcatcaac accggattat tgctattttc atcctggaag ctcacgccaa
     5521 tggagccgcg agtcactttt ccggacgtga ccagttggtt gtacacagtg atcgccgtat
     5581 tcgatggcag cgcgaaacca acgccttcaa atccgcgact tccagtgtaa atcgcggtgt
     5641 tgatgccgat tacttcgccg cccagactca cgagcgcccc accggagtta cccggattga
     5701 tagcggcgtc ggtctgaata aagcgctgaa actgatggcc tacgttcgag cggtctttcg
     5761 cgcttacgat tccggcggtg acggtcgaat tcaatccgaa cggactgccg aatgccaaga
     5821 cccagtcgcc gacttcgaca ccatccgaat tgcccagctt cgccacgggc aaatcgtggt
     5881 tcgcatcgat tttgatgacg gcgaggtcgg tctctttatc agtaccaatc acgcgagcat
     5941 tgtaatgacc gggatcgcca tcgagcgcga cctggatttt ggtggctccg tcaatcacgt
     6001 gatcgttggt aagaatgaaa cctcgcttgt ctaccaagac gcccgaaccg agacttcgtt
     6061 cagcatcagg agcattgtcg ggcgagttga agaagcgatc gaagaaatcc tgaaagggat
     6121 cgttttggcc gtggggcgca tgcccgccct tcggcttttc gattacctga gttgtggaga
     6181 tgttgacgac ggccgggccg aggctcttcg agattttggc aaaagccccc gacaagctca
     6241 ccggatctgg tacggccagc agcgacgcgc cgttggcacc ttgcgcgcga ttggccagcg
     6301 catgtccgga aaaactcgca ccgataacaa tgccgacgca gagggtcaat gccaccagga
     6361 gcgacgcgaa aattttccgg cgcctaaccc aactccacag ctcccgaaat tggatcccca
     6421 tcccctgctc cttgtcagac actaaaaagt ctgcgtgatt ataacactca ttccgccggg
     6481 gcatcgccgg gttcgagcgc aaatgccaag attgacaacg aagccagcac agcggtttca
     6541 gtgcggagga tgtttttccc aagcgaagcc tccgcaaatc cggaggcgcg ggcggcctcg
     6601 atttcttggg cggtccagcc cccttccggt ccgatggcaa gggcggcaga aacgccttcc
     6661 cttagcggct gaagccgctc ggagtcggca cggcttgcgg cacgaccgaa gtcgtgccct
     6721 gacgaagagt cgggttgcaa ccaagattct gaagtcccgg cgagtacggc gcgaagcgga
     6781 ggagcatccg agcgttccga gagcacgatt ctcaagcctg ctgaatactt ccgaaaagct
     6841 tgctccggag ttccaatttc gtgcagcacc ggagggccca ggcgccgaga ctgttgggca
     6901 gactcgcgca gaatcttttg ccaccgttca tggcgcttgg ctgcggcagc gacgagaggt
     6961 ttatccgtgc gcgccgcggc gagcggaaca atttcattcg cgccgagttc cgtagccttc
     7021 tccaggcacc actcgaagcg gtcaaatttc actagcgaaa tcaggagatc gatccgcagc
     7081 gagggctcgg atgtgggaac cgtttcgatc aacgcgaatt cgatgcgact cttgccgcgc
     7141 tttgcgagcg ccactcgctc tattttcccc aaccaaacgc ggtggccatc gctcagttca
     7201 tagaactggc ctggctgggc gcgaaggaca cgacctaaat ggtcggccgc ttcgccgcgc
     7261 aggactgcgg cagcatccga aaagttctcc acgaaaaagc ggcgacgcat gcgcggattc
     7321 tagcaagaaa gccacgcttc agcgcgaatg ctaaacttcc tccatgcagc acagcgaaac
     7381 aaacctgcag cggcaatggc ccgcgcctcg caggtcgtgg ctgatgaaac aggtgtggca
//
LOCUS       DATNDT010000523         3837 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2189183, whole genome shotgun sequence.
ACCESSION   DATNDT010000523 DATNDT010000000
VERSION     DATNDT010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3837
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2189183"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..672
                     /locus_tag="VN788_16980"
     CDS             211..672
                     /locus_tag="VN788_16980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013029464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HXU22275.1"
                     /translation="MKPTEMEILLVEDNPKDLELTLHALRKENLNNCIEVVRDGEEAL
                     DFLFCRGSHSSRSFESPPKLIILDLKLPKVDGLEVLRAIKNDARTRAIPVAILTSSNE
                     DRDLVDGYKLGVNSYIRKPVDFDQFRKIVKQLGLYWLIVNQSPPATAFSER"
     gene            686..3046
                     /locus_tag="VN788_16985"
     CDS             686..3046
                     /locus_tag="VN788_16985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU22276.1"
                     /translation="MLTPIDQTMGHVHDGHSVPARLRVLILEDNQRDAELFIQKLKEA
                     GCALDADTVDSEDAFVASLRSRVYDLILSDYSIPEWSGLEAFRFVKRSGINIPFILVT
                     GTLGEEAAVDLIKEGVADYILKDRLARLPSAVRRALQEKLIRDEAGRARHALHDSEEK
                     VRLLLDSTAEAIYGINLEGNCTFCNPACARLLGYDNPSDLLGKQMHSLSHHARVEGTP
                     RSIDNCPICLAARAGRGIHLDERALWAKDGSSFPAECWSHPMLQDGKSIGSVVTFFDI
                     TERKQIEDNLRRVAAVVESSQDAIISQTLDGIILTWNLGAEQIYGYSRAEMLGKSLAM
                     IVPPDRQSEFHGILKKISWGEKVEQLQTVRVKKDGTKVHISLSVSSIKDANGKIVGAS
                     AIARDITETKHLEEMFLQAQKMEAVGRLAGGVAHDFNNLLGVIIGYSDIVEGRLKEDD
                     PVRAKVLQIKKAGQGAARLTRQLLAFSRQQILDSRILDLNSVVTDVQKMLQRLIGADV
                     EMVTSLCPDLAPMKADQGQIEQVIMNLIVNSRDAMPDGGTLTIETSNVELDALYVQSH
                     PELTAGSFVMLAVTDTGTGIDPEIQKHIFEPFFTTKEQGKGTGLGLSTVYGVVKQSGG
                     SISVSSEVGRGTTFRIYLPQTHAVAERDSVESKRLFEGTETVLVVEDEESLRKLTCEM
                     LLENHYSVLEASNGFEAHEISQLHEGPIHLLLTDVVMPRISGPSLAKSLVARHPNMKV
                     LYMSGYMNSSVVQELAISGSEFLQKPFTSDTLTRKVREILGATVLG"
     gene            3179..>3837
                     /locus_tag="VN788_16990"
     CDS             3179..>3837
                     /locus_tag="VN788_16990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22277.1"
                     /translation="MLGSVFSFPFSLAMPNEALAQPIRKILFYAALLAIFLAVPHFAA
                     AQLPPKSPPSLFTGGGEATLEADQQRESGKVFYADGHVEIRYENARLRADHVEYDSAS
                     QIVSARGHVQLDYRTQHVEADDARYELRTGRGTFHHARGTFALQRRPSPTLLLSPNPL
                     YFDAEEAERVDQNTYRVRKVWLTICKPGRPTWKFYASSATVRLQKSVHLENGDFRVFS
                     IP"
BASE COUNT          911 a         1104 c          999 g          823 t
ORIGIN      
        1 ggtagcgacg gaaaaggtct acagtttctt tcaggaatac ctagcagaaa gcacgacgaa
       61 cacgcgtttg attttgcgac agacttttcg ctcgcctaaa tcatcgctcc cctctttgtt
      121 cctcatgctg ttgccccgaa gctttgtggt cgatctataa gatgaagtga ccgcgtcatt
      181 atgcccagtg acgcgcaatg gaggcaccac atgaagccaa ccgaaatgga aattctgctg
      241 gtcgaagata atcccaaaga tctcgagttg actctccacg cgctccgcaa agagaacctc
      301 aacaattgca ttgaagtggt gcgtgatggc gaggaggctc tggacttcct attctgccgg
      361 ggatcccaca gcagccgctc ctttgagagc ccgcccaaac tgatcattct cgatttaaaa
      421 cttcccaagg tcgatggact cgaagtgttg cgcgcgatca aaaacgatgc ccgtacgcgc
      481 gcgattcccg tagccatact gacgtcatca aacgaggatc gcgatctcgt cgacggctac
      541 aagctcggcg tcaacagtta tatccggaaa ccggttgact ttgaccaatt tcgaaaaatc
      601 gtaaagcagc ttggactcta ctggttgatt gtcaaccaat cgcctcccgc tacggcattc
      661 tccgaacggt gaaagaggac cctgtatgct cacgccaata gaccaaacta tggggcatgt
      721 tcacgatggt cactccgtcc cagctcgcct gcgcgtcctg attctcgaag ataatcagcg
      781 ggacgctgag ctcttcattc agaaactgaa agaagcaggc tgcgcactcg acgcggacac
      841 cgtggatagc gaggacgcct tcgtggcgag tttacgatct cgggtttacg atctcatcct
      901 gtccgactac tccattcctg aatggagcgg cctggaagcc ttccgcttcg tgaagcgcag
      961 tggaataaat attcccttta ttttggtgac cggcacactc ggagaagaag ccgcggttga
     1021 cctgatcaag gaaggagtag ccgattacat cctgaaagat cgtttggccc gcttgccatc
     1081 ggctgtacgc cgggctctgc aggaaaaact aatacgcgac gaagccgggc gagcacggca
     1141 cgccctccac gatagcgaag aaaaagttcg gctattgctc gattccaccg ccgaggccat
     1201 ctacggaatc aatctcgagg gaaactgtac tttttgcaat ccagcctgcg cacggctgct
     1261 cggatacgat aatccaagcg acctgctcgg aaagcagatg cattcgctca gccaccatgc
     1321 tcgcgtcgaa ggtacgccgc gctcgattga caattgcccg atttgcctgg ccgcccgtgc
     1381 gggtcgaggg atccatctgg atgagcgggc tctatgggca aaagacggct ccagctttcc
     1441 ggcagagtgc tggtcgcacc ccatgttgca ggacggcaag tcgattggtt ccgtcgtaac
     1501 gttcttcgac ataaccgagc gaaagcaaat tgaggacaac ctccgccgcg tggccgcggt
     1561 tgtggaatct tcgcaggacg caatcatcag tcagactctg gacggaatca tcctcacttg
     1621 gaacctcggc gccgagcaaa tctacgggta ttcgcgcgcc gaaatgctcg gaaaatcgct
     1681 tgccatgatc gttccccccg accggcagag tgagtttcac ggcatcctca aaaaaattag
     1741 ctggggcgaa aaggtcgaac agctccaaac agtccgcgta aaaaaggacg gaaccaaagt
     1801 tcacatttcg ctctctgtat cctccatcaa ggatgcgaac ggaaaaattg tcggtgcttc
     1861 agcaatcgcc cgcgacatta ctgagaccaa acatttggaa gaaatgttcc ttcaggcgca
     1921 aaaaatggaa gccgtcggcc gattggcggg tggcgtcgct cacgacttca ataatctatt
     1981 gggcgtaatc atcggttaca gcgatatcgt cgagggacga ttgaaagaag atgatccggt
     2041 ccgcgccaag gttttacaaa ttaagaaagc gggccaaggc gccgcaagac taacccgcca
     2101 gcttctggcc tttagccgcc agcaaatact tgattcaaga attttggacc tgaattcagt
     2161 cgtcacagac gtccaaaaga tgctccagcg cctcatcggg gcggacgtag aaatggtcac
     2221 atccttgtgt cccgatttag cgcccatgaa agcggaccaa ggacagatcg agcaggtaat
     2281 catgaatcta atcgtcaatt ccagggacgc gatgcccgac ggtggaacgc tgacgatcga
     2341 aacctcgaat gttgaattgg acgctctcta tgtacaaagc catccggagc tcaccgctgg
     2401 ctcattcgtc atgcttgcgg tcaccgacac gggcaccggc atcgatccgg aaattcagaa
     2461 gcacatcttc gagccattct ttaccacgaa agagcaggga aagggcactg gacttggcct
     2521 ttcgacggtc tacggggttg ttaaacagag cggcggctcc atctcggtat ccagcgaagt
     2581 gggccgcgga acgactttca gaatctacct ccctcaaacc cacgcagtag ccgagcgcga
     2641 tagtgtcgaa tcgaaaagat tatttgaagg aacggaaaca gtcttggtag tcgaagacga
     2701 agaatcgctc aggaagctca cttgcgagat gcttttggaa aatcactaca gcgttctgga
     2761 agccagcaac ggatttgagg cgcatgaaat ttctcaactt cacgagggcc ccattcatct
     2821 ccttcttact gacgtggtga tgccgagaat tagtggtcca agcttggcca aatctctcgt
     2881 cgctcggcat cccaatatga aagtgctcta tatgtcgggt tacatgaact cctctgtggt
     2941 ccaagagctg gccatcagcg ggtcggaatt tctccagaaa cctttcacca gcgacacgct
     3001 tacccgcaag gtccgggaaa tattgggggc taccgttctg ggctgaccgc gcctctcgca
     3061 gctgccgttt cagcctggca ccaccggggc gctgcaacct gcagccgccg ttcctgcatc
     3121 gatgtgatcg attgccgcgc gtcctcgcgc cctaacggat tgcgggcaag cggggcgcat
     3181 gttaggctcg gttttctctt ttccgttttc tctcgcaatg cccaacgagg ctctcgcgca
     3241 acctatccgc aaaattttgt tctatgccgc gctgctggct attttcctcg cggtgccgca
     3301 tttcgcggcc gctcagctcc ccccaaaatc tccacccagc cttttcaccg gcggcggcga
     3361 agccactctg gaagcggacc agcaacgcga atccggaaaa gttttctatg ccgatgggca
     3421 cgtcgagatc cgctacgaaa atgcgcggct tcgcgcggac catgtcgaat atgacagcgc
     3481 ctcccagatc gtctccgcgc gcggccacgt gcagctcgat tatcggacgc agcacgtcga
     3541 ggccgatgac gcccgctacg aattgcgcac cggacgcggc actttccacc atgctcgcgg
     3601 aacattcgcc ttgcagcggc gcccctcgcc cactttgctc ctcagcccca atccacttta
     3661 ctttgacgcc gaggaagcag agcgcgtgga ccaaaacacc tatcgcgtcc gcaaagtatg
     3721 gctcaccatc tgcaagccgg ggcggcccac atggaagttt tacgcaagct cagctaccgt
     3781 caggttgcag aaatcggttc acctcgagaa cggagatttt cgcgtttttt cgattcc
//
LOCUS       DATNDT010000524        12602 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1474711, whole genome shotgun sequence.
ACCESSION   DATNDT010000524 DATNDT010000000
VERSION     DATNDT010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 12602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12602
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1474711"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..461)
                     /locus_tag="VN788_16995"
     CDS             complement(246..461)
                     /locus_tag="VN788_16995"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HXU22278.1"
                     /translation="MDRNPASEQIEARAYEIYLERGREDGHDVEDWLAAEKELSQRRE
                     EVNALLEQEQPESARLGYAASAAGRRK"
     gene            complement(619..1695)
                     /locus_tag="VN788_17000"
     CDS             complement(619..1695)
                     /locus_tag="VN788_17000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU22279.1"
                     /translation="MIGRKYLLALLVPGIVISSTAVITAHAARQNPPDIRMSVEMVQL
                     NVAVTDRRGKYITGLKPSDFYVAEDGIPQNMAMFAEGDNAPRRLSEVVAATDTKDSQP
                     IGATASLPAVPEANDPADNSDDSPLAGASVFILFDTSNYMYHGFVYAQDAIADFVQSL
                     QGPDRVALYSYSRDLFRASSLTPDRLKVLRSVRETVAGDNAALYDAILLTLRDAGTIT
                     GRRVIVVFSNGPDNASLAPPEDVEELAQSEGVAIYMISTHQAEQDPLSNKVFNRISSS
                     TGGEVYFAKNWTEEKKAFTAIRNDLSHLYTLYYYPQPNPNRGWRAITVKLKGPGEGKY
                     RIRARSGYRPLPVHQAAGEDSATS"
     gene            complement(1778..4942)
                     /locus_tag="VN788_17005"
     CDS             complement(1778..4942)
                     /locus_tag="VN788_17005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22280.1"
                     /translation="MCLFLRLRVVSLFVLICACALCGEVARSQPSPQGSSAISAQSEF
                     SPQTNTAAAGEAKPEQSSTNYILIPGPLRPFLRMSGISQQVTPDQVLPLFGHFVETYG
                     FEGSKEKSPKATEALLIFRRYFVQANTLTSLAGAKQTLGFSDCENSRQLLADLGYKLE
                     NGCKGIPAIEVDDPEKAFITVDSGFPLADLQLALQQGKPFTTPYASTRVPLIYTKADW
                     TGKANSTDVDVIDALDQNASLSRLYWGLSRVDNNAREILLRSGGVQKLLPYASVLDFY
                     GPSLAVRDGRVAVPGGSVAEPAWKKLVGADPGDPAKFIYGLLAKDGGWLAEYYDAISR
                     APDRQLQYFTTDGRLQRFYDAFHGNDTSHDSVRSIFRPGADLLLLTARLPLDDSGEPI
                     VPGGIAAWAGAFRKKSDSKMERELSRKVEHWKNPDQFMEGMFALARFNSTNGPLETYE
                     TLSEIDRNRASAARLNAETTRLLIAHYGKLHDQYGMFAEFSGLNDASIALFVKATEKI
                     DAIREPMLRADTMGIFEANVGLWQIFARQGQIAPSDLNTSWRQTIEPFGTFGVSAQLF
                     DATRSSLTAVVTAASGDSNVSQQKIIDLLAGPQMPTVDSRQVHDRLANDIRTVMTDQR
                     LVPLDTLLELGDDFAALTHTPNGVDAARAQGLAEELEEARAPRAMFTEAERAEWAPGH
                     EPNHHVMIELHTDLRKAISGSEPPKQASLMRGALTPFLRDTLVGLNYAYYQPPGAQIL
                     HNSPLLVREHDFLASESVGQNRAWEAPQLFGIGLTAGNGTHLSGSLAGLPYALAEIEQ
                     DFIVPENVQALIWHDTAADLLTTSVVPRWWSTSQDALHAASLYQQAGEELVDRAAKDS
                     SLRPEVSTILADRLSPEAMDAVNAELAAGRADEALRRIAPADEFYLTEEFRKKFPDRA
                     GVWGTAGTELEALAKRDPSQVDPKVLSQDFGVPHPTITQNYADSILNLRPFPSVMDYA
                     SELLAESWESTNLYWARLADEMGYTPVMLNELAPVLTRRMVEKIFGSDFDDWPALLRA
                     MRETGTEFRKGQVAGVPKINMPLP"
     gene            complement(5301..7637)
                     /locus_tag="VN788_17010"
     CDS             complement(5301..7637)
                     /locus_tag="VN788_17010"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HXU22281.1"
                     /translation="MRKATAIRVFTSGSILATVILSGVLIAQQRLPQRTASAPITGAY
                     TSVKTAPNSYVDGAWCAQCHADIAKTYALTGMSRSFSKITADTTEEKFPDGQAYFHAA
                     SQSYFAIIKRDGEVFERRWQSGFNGQETNVEEKRIDYVLGSGNHAKTYLHLTARNTLQ
                     QLPLGWYAENGGTWGMIPGFDRADYPGSTRLVHYECMFCHNGYPKVPDGHAEEDAEPV
                     FEQPLPTGIDCQRCHGPGQRHIETVGKPGATAQEIRASIVNPGRLSPEREMEVCMECH
                     LETTSLLLPHSIQRVNRGPFSYIPGQLLADFRLSFDRAPGQNKRFEVAGAAYRLRESQ
                     CFLQTQKNDDAHRLTCTTCHNPHDIPRGEAAKTHYNAVCNECHATTIAQAVVEGSHPS
                     DTDCISCHMPKRRTDDAIHIVMTDHLIQRRPPPDLLAAKNEYYETPATSYKGEVTLYY
                     PPSLAPTPLNQLDVAAAQVKDESNLREGIPKLATLIQKYHPAGATYYVDLADALHSAG
                     NAAASTAMYRTALQHAPDSSVILLKLGVAQVDWQQWSRAETTLRGLVDKTPNDPVAWV
                     LLGQALFQENKNAEAREALTKAVSLDGDMAEPHNYLGGLLVRSNDLAGAEKEFREAVR
                     LQPNHAEWQSNLAGLLAEERKIPEARYLFELSIRLNPNFAGARLNYGRLLAALGENVN
                     AEKQAQAAVEADAKNPAAHELWGYLLTATGDPQGATRELRSAVRLQPDFWRAHFELGV
                     ALRMTGDAVGAAQELRIAARGNDPEVRAAALQQLNAGR"
     gene            complement(7704..7994)
                     /locus_tag="VN788_17015"
     CDS             complement(7704..7994)
                     /locus_tag="VN788_17015"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HXU22282.1"
                     /translation="MRKSRFEAEEIIELLNEAASGVLVRKICIRIGVSEATFYRWRTQ
                     YQGLAASGIRRLRELEQENSRLRRDLGNKNLECDVLRAELSGNQVHQLVSQP"
     gene            complement(8086..12264)
                     /locus_tag="VN788_17020"
     CDS             complement(8086..12264)
                     /locus_tag="VN788_17020"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU22283.1"
                     /translation="MRRKFGDRYFRTEGRYRLLSIVAVLTLLLGVCASELPAQTINGQ
                     IAGTVTDPTGAVIVGAKVTLTYPLTGQQRVVQTSSSGDFVFPDLVPGTYDISIAQSGF
                     QTYSQKGIAVAPTEKVALHTIPLSVGNVSTEVTVQASATHVETDSSEHSALVNTTQMT
                     DVTVKGRNYMSYIALLPGVTNNKPGGGDAPGWDQTDGLTINGGNNAVIVMLNGIASSD
                     DGIGNSSAAYLAPSTDAIQEMKVQTGNLNAEYGARNGGSINVMFKTGTNQFHGDLYDY
                     ERNRFFNANTYFNKESANPYTRAHPDSYTYHNPGGTIGGPFWLPKVPFNKNRDKLFLF
                     FSADLLRRNTGSTSTALVPSQEERMGNFSEVASNPLGQTGAIAGCVDSKGKNLAGCSV
                     PANIFGLGNPAFAGTEVYCPGTEPTGGANQTSSGSNPYLSPAQAANLTVACGANAITA
                     APWAGAMGSVNPLLNVFPLPTCNSDSDLYNGGLGDGTAAKSANPTLNLSACGAGLGSA
                     YSNNYNYTLLSVTQNPRSDYVLTGQYNLSKNNIWTVDLEKGYQCTCGGTFLGAGNGWN
                     ELTTNYEIHGGEVSSNLVSTLRPNLVNEFIVGTTRALQTVFPANTTNKNAYGYFGRNE
                     RNLDGLGPSVLPVIFPDPSNSKFGANSQSLAANPFNYIPTTSYGNSYAGTSGDNRYPF
                     FGTDTHYNLQDDITWIKGAHAIKGGFYFEKVSRNGPAGGGALGGWNGNVNFQGNSTNP
                     NDINLGLANAYYGVFQSYNETSTHPNGYDRFHGEEWFIQDTWKITRRVTLDYGMRFAH
                     DVPSFDTVLISDFRPDVYQASAQPGLIAPCMVGGVRMGCYGSTTFTQSAIGQFAPIAV
                     SGVTPFQGIVAYTPGTPVMNTPPLNILPRFGFAWDVFGNGKTALRGGFGITNNVFGVV
                     DTVGQLVLSPPSPALLASQPIPSTNKQQLPISPTIYNSTLPTMLANQSNPNAACSVTS
                     ATSDCFIGPQNVIGLPRNFKDPQSYNYSLGIQHDLGHGLLLDVTYVGIQNRHNYNNSG
                     IAQDVEPYGIQWLETDGSYARAANKSSATNPSGCTPAPGVTLAFGDPTKGGCTLLPSV
                     FYNYPSINNGYTIQHANNLGILNTNLPGYARVNQIYDNLNSNYNSLQSQVSKRFGRTV
                     TLNLSWTWAKEMQWSEPSLIAPRSVWRDLYYNQSGPKHNIVANWTYNLPQTHFSHGIF
                     NEAISGWVFSGTFEYVTGTAGTVGAGSGINYNGGGGYGTRVSLVPGQSVYNKHGVPPG
                     GGKALKEQYLNINAFAAPNGGQGICDGTAANCGFGNSGRINYIGPSTNNFDMSIFKNF
                     AMSKTNEARKLQFRFESYNTFNHTEFTTLSTGLTTNTANVPFTQGGGNNTTFGRFTAT
                     QPARILSLGLKFLF"
     gene            12289..>12602
                     /locus_tag="VN788_17025"
     CDS             12289..>12602
                     /locus_tag="VN788_17025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22284.1"
                     /translation="MCDPRVFMLARPTPKPTNSQWLGRFRRSSNTNAQASERQVQKRI
                     ASPCPDNLALLTTTRLENACYQLKLFISGNEEVSFGPGNVNHTRLNSFFKRIYLNASS
                     LIR"
BASE COUNT         2594 a         3456 c         3684 g         2868 t
ORIGIN      
        1 ggttcggcag gtatctggga agtcgaaata atggggggca aagtcggaga gacgggtact
       61 tgagaaggcg ctgcaggtgt ctgagaaagg cgggagcctt tcagcggcgc acaaattgcc
      121 accgcgagca caaaaagaac gacgaggcaa acaaaaagcg gtttccgcac ggaaagcaag
      181 cataacagaa tccgccggaa agaatcgtcc gcactcagtc aaattcaacg agcgtatcgg
      241 tctcgttact tccgccgccc cgccgcgctg gcagcgtatc ccagtcttgc ggactcaggt
      301 tgttcctgct ccaagagtgc gtttacttcc tcgcgacgtt gcgaaagctc tttctccgcc
      361 gccagccaat cttccacgtc atggccatct tctcggccgc gttcaagata aatttcgtat
      421 gcgcgagctt caatctgttc gctcgccgga ttgcggtcca tgaactctgc ctcactttcg
      481 gtctcaaatt aggcgcgtgg ctcgagcgga acaatcgggt gttccgtgta agagtcccaa
      541 gttagcttgg gtgcggtttg aatgaggcgt tacaagcgta ttgcgatgaa cacaaagaat
      601 gcagacgaag cagaaagcct agcttgtcgc gctgtcttct ccggccgcct gatgaacggg
      661 cagtggacgg tagccgctgc gcgcgcggat gcggtatttg ccttcaccgg gacccttgag
      721 cttcacggtg atggcccgcc acccgcgatt gggattcggc tgcggataat agtagagagt
      781 atagagatgc gacaagtcgt tgcgaattgc agtgaacgct tttttctcct cggtccaatt
      841 tttggcgaaa tagacttcgc ctccggtgga cgagctgatg cggttgaaaa ccttgttgga
      901 aagcggatcc tgctccgcct gatgcgtgct gatcatgtaa atcgcaacac cttcggattg
      961 tgcaagctct tccacgtcct ccggcggcgc aaggctggca ttgtccggac catttgaaaa
     1021 tacgacgatc actctgcggc ctgtgatggt gccggcatca cggagcgtga gcaaaatggc
     1081 atcgtagagc gcggcgttgt cgcctgccac cgtttcacgg acgctgcgaa gcaccttcag
     1141 gcgatccggc gtcagcgacg aggcgcgaaa aagatcgcgg ctgtaagaat agagagcaac
     1201 gcgatcgggc ccttgcagcg actgaacgaa atcggcgatc gcgtcttgcg catagacaaa
     1261 gccgtgatac atgtaattgc tcgtatcgaa aagaatgaat acgctggcgc cggcgagagg
     1321 cgagtcgtcg ctgttatctg ccggatcgtt tgcctctggc acagcgggca gagatgctgt
     1381 agccccaatg ggctgagaat ccttcgtatc agtggctgcc actacttcgg aaagtcttct
     1441 tggtgcgttg tcaccctccg cgaacatggc catgttctgc gggatgccgt cttcggcgac
     1501 ataaaaatcg cttggtttca gccccgtaat gtacttgccg cggcggtccg tcacggcgac
     1561 attgagctgt accatctcaa cgctcatgcg aatgtccggt ggattctgcc gtgcggcatg
     1621 ggccgtgatt acggccgtgg agctaatgac gatgcccggt acaagcaacg caagaagata
     1681 ctttcttccg atcatgtttg cctcacacgc ccttcgcggg cattccgttc cgcatcggca
     1741 tcaaactttc ctcgcagtgg cggcccgcca aacgtcgtta gggaaggggc atattgatct
     1801 ttggcacacc ggcgacttgc cccttgcgaa attcagtgcc ggtttcgcgc attgcccgaa
     1861 gaagcgccgg ccaatcgtca aaatcgctgc caaagatctt ttcgaccatg cggcgcgtca
     1921 gcacaggcgc cagttcgttg agcatcacgg gcgtgtagcc catttcgtcc gccaggcgcg
     1981 cccaataaag gttcgtcgat tcccatgact ccgcgagcag ctcactggca tagtccatca
     2041 cagaaggaaa cggccgcagg ttcagaatcg agtcggcgta attctgcgtg attgtcggat
     2101 ggggaacgcc gaaatcttgc gagagcacct tagggtcaac ctgcgaaggg tctcgcttgg
     2161 cgagtgcttc cagttccgtt ccggccgtcc cccagacacc ggcgcggtca gggaatttct
     2221 tccgaaactc ttccgtgaga taaaactcat cggcgggcgc gattcttctc aacgcctcat
     2281 ccgcccgtcc ggcagcgagt tcggcattta ctgcatccat tgcctcgggc gaaaggcgat
     2341 ccgcgaggat tgtgctgacc tccggccgaa gagaagaatc ttttgcggcg cggtccacaa
     2401 gctcctctcc ggcttgttga tagagagagg cggcatggag agcgtcctgg gaagttgacc
     2461 accagcgtgg aactacgctc gtggtcagta ggtctgctgc ggtgtcgtgc cagatcaatg
     2521 cctggacgtt ttcaggaacg ataaaatcct gctcgatttc ggccagtgcg taaggcaatc
     2581 cggcgagtga gccggaaaga tgcgtgccgt tgcccgccgt caatccgatg ccaaagagct
     2641 gaggcgcttc ccatgcgcgg ttttgtccga cggactctga cgccagaaaa tcatgctcgc
     2701 gcaccagaag cgggctattg tgcagaatct gcgcgccggg cggctggtaa tatgcgtaat
     2761 tcaggccaac cagagtatcg cgcagaaaag gcgtgagagc gcctcgcatc agcgatgcct
     2821 gtttgggcgg ctcggaaccg ctgatcgcct tgcgaagatc ggtatgtagt tcgatcatca
     2881 cgtgatgatt tggttcgtgt cctggtgccc actcggcgcg ttcggcttcg gtgaacattg
     2941 cacgcggcgc tcgcgcttct tccagttcct ccgctaaacc ctgcgcacgt gctgcgtcaa
     3001 ccccgttagg cgtgtgcgtc agagccgcga aatcgtcacc gagctcaagc agggtgtcta
     3061 gtggaacgag acgctggtcg gtcatcactg tacgaatatc gttcgcgagc ctgtcatgaa
     3121 cttggcgtga atcgacggta ggcatctgcg ggccggcaag caaatcgatg atcttctgct
     3181 gagagacatt cgaatcgccg gaggccgctg tcacaaccgc ggttagcgaa ctgcgcgtcg
     3241 catcaaaaag ctgcgcagag acgccaaagg tgccaaacgg ttcgatggtt tgccgccagc
     3301 ttgtattaag gtccgacggt gcgatttggc cttgacgtgc aaagatttgc cagaggccga
     3361 cattcgcttc gaatatgccc atcgtatcgg cgcgaagcat cggctcgcgg atggcgtcga
     3421 ttttctcggt tgccttcaca aagagtgcga tcgatgcatc gttcaggccc gaaaattcgg
     3481 caaacatccc gtattggtca tgcagctttc cataatgcgc gatcaaaagg cgagtcgttt
     3541 cagcgttgag ccgcgctgcg ctggcgcggt ttcggtcgat ttcgctcagg gtttcgtagg
     3601 tttcgagtgg gccgttcgtg gagttgaacc gtgcaagagc gaacattccc tccatgaact
     3661 ggtccggatt cttccaatgc tcgactttcc gggagagttc gcgctccatt tttgaatccg
     3721 attttttgcg gaaggcaccc gcccaagcgg ctatcccgcc gggtactatg ggctcaccgc
     3781 tgtcgtcgag cggaaggcgc gccgtcagca gcagaagatc ggcgccgggc cggaagatcg
     3841 agcgtaccga atcgtgggag gtgtcgttgc cgtggaacgc atcgtaaaag cgctgcaggc
     3901 ggccgtctgt ggtgaagtat tgaagttggc ggtccggcgc gcgcgaaata gcatcgtaat
     3961 actcggcgag ccagccgccg tctttcgcca gcaagccgta gatgaactta gcgggatccc
     4021 ctggatcggc gccgaccaac ttcttccacg ctggttcggc cacactgccg ccgggaaccg
     4081 ccacgcggcc gtcacgcacg gcgaggctcg ggccgtagaa atccagcacg gaggcatacg
     4141 gcagcagttt ctgcactccg ccgctacgca gcaggatttc acgcgcgttg ttgtccacgc
     4201 gcgaaagtcc ccaatacagc cgcgatagcg aagcgttctg gtccagcgca tcgatcacgt
     4261 ccacatccgt gctgttcgct ttaccggtcc agtccgcttt cgtatagatc aagggaaccc
     4321 gcgttgaagc gtacggcgta gtgaagggtt tcccttgctg caacgcaagt tgaaggtctg
     4381 cgagggggaa tccggaatcg acggtgataa acgccttctc gggatcgtct acttcgatcg
     4441 cgggaatccc tttgcatcca ttctcaagct tgtaacccaa atcggcgagc agttgccgcg
     4501 aattttcgca atcggaaaac cccagcgtct gttttgctcc cgccagagaa gtcagcgtgt
     4561 tcgcttgaac aaaatagcgt ctgaatataa ggagagcctc ggtggctttc ggtgactttt
     4621 cttttgaacc ctcaaagcca tacgtctcga cgaaatggcc aaagagcggc aacacctgat
     4681 ctggcgttac ttgctggctg atcccggaca tccttagaaa cgggcgcaaa gggcccggaa
     4741 tcagaatgta attcgtggag gattgctccg gttttgcttc tccagcggcg gcagtattgg
     4801 tttgcggcga aaattcagac tgcgcggaaa ttgcggagga accttgcggt gaaggttggc
     4861 tgcgcgcaac ttcgccacaa agggcgcacg cacagatgag cacaaacaaa gaaactactc
     4921 gcaagcgaag gaagagacac atggccttga tgctccttcc ggccctgcac cgatgggttg
     4981 tttaacccgc ctggaacagc gggtacggtt ttatgctccc gggttacgaa accaccgaat
     5041 atgaccagcg cagaatcgtc ggagtcttgg tggcaactgt gggctggccc aaaactgccg
     5101 catagcgtaa accattcggt cgtcaattcc aacagattct cgctacggtc tattgggtag
     5161 cgatgccgcg gtggtcacgg tagtgcgcgc agttatccgc gtaaaaatcc acaggtcttt
     5221 tagccttgtg ctggccgatt cgtgtttctg ttttgtcata gctttgtttt tgtttcgtca
     5281 ccaatccggt tgctctatct tcaacgtccg gcgttcagct gctgaagtgc cgcagcccgc
     5341 acttcaggat cgtttcctcg tgccgcaatc cgcaattctt gggcggcgcc aaccgcatcg
     5401 ccggtcatcc gcaaagcaac accgagctcg aaatgggcgc gccagaaatc cggctgaagc
     5461 cggacggcgc tgcgcagctc gcgagtcgcg ccttgaggat cgccggtagc cgttaacaaa
     5521 tatccccaaa gctcgtgcgc ggcaggattc ttcgcgtccg cttccactgc tgcttgcgcc
     5581 tgtttttcgg cgttcacgtt ttctccaagc gccgcaagga gcctcccgta attgagccgg
     5641 gccccggcaa aattgggatt gagccgaata ctcagttcga aaagataccg cgcttccggg
     5701 attttccttt cctctgcaag caggcccgca agattcgact gccattccgc atgattcggc
     5761 tgcagccgca ccgcttcgcg aaactccttt tcagcgcctg ccaaatcgtt gctgcgtaca
     5821 agcagaccgc cgagataatt gtgcggttcg gccatatcgc catcgaggct cacggctttc
     5881 gtgagagctt ccctggcttc tgcgtttttg ttttcttgga agagcgcttg gccgagcagc
     5941 acccacgcaa ccggatcgtt cggtgtcttg tcgacgaggc cacgcagtgt cgtttccgca
     6001 cgcgaccatt gctgccaatc aacctgcgcg acacccagct taagcagaat gacagacgag
     6061 tccggagcat gttggagagc cgtacgatac atcgccgtgg atgctgccgc gttccccgca
     6121 gaatgaagcg cgtccgcaag gtccacataa taggtagcgc ctgcgggatg gtatttctgg
     6181 atgagggtag ccaacttcgg aataccctcg cgtagattgc tctcgtcctt tacttgagcc
     6241 gcggcaacat cgagttgatt taggggtgtc ggcgcgaggc tcggcgggta atagagcgtc
     6301 acttcgcctt tgtaggaagt cgctggagtc tcatagtact cattcttcgc ggcaagcaaa
     6361 tccggcggtg gacggcgctg gatcaaatgg tccgtcatca cgatatgaat ggcatcatcg
     6421 gtgcgccgtt tcggcatatg gcaactgatg cagtccgtat cggagggatg tgaaccctcc
     6481 acgacggcct gcgcgattgt agtggcgtga cactcgttgc agaccgcgtt gtaatgcgtt
     6541 ttcgctgcct cgccacgtgg gatgtcgtgc gggttatggc atgtcgtgca cgtcagccgg
     6601 tgtgcatcgt cattcttctg cgtctgcagg aagcattgcg actcgcgtag ccggtaggcc
     6661 gcgcccgcca cttcaaagcg tttgttctgg ccgggtgcgc ggtcgaacga cagccggaaa
     6721 tcggcaagca gctgtccggg aatatatgag aatggccccc gattcacgcg ttggattgag
     6781 tgcggcagca gcaggctggt cgtttcgaga tggcactcca tacaaacttc catttcgcgc
     6841 tcgggggaaa gccgccctgg attcacgatt gaagcgcgta tctcttgcgc agttgcgccc
     6901 ggttttccga ccgtttcgat gtgtctctgg cccggtccat ggcagcgctg acaatcgatt
     6961 cccgtgggaa ggggctgctc aaaaaccggc tcggcgtcct cctccgcatg cccatctgga
     7021 actttcggat agccgttgtg gcagaacatg cactcgtaat gaaccagtcg cgtcgagcct
     7081 ggataatccg ccctatcgaa gccgggaatc attccccacg tgccgccgtt ttccgcgtac
     7141 cagccgagcg gcagttgctg gagggtgttg cgcgcggtca gatgcaagta cgttttcgcg
     7201 tgattacctg agcccagaac gtaatcgatc ctcttctctt caacattcgt ttcctgcccg
     7261 ttgaatccgc tttgccaacg gcgttcgaac acctcgccgt cgcgtttgat gatggcgaaa
     7321 tagctctgcg aggcggcatg gaaatacgct tggccatcgg gaaacttctc ctccgtcgta
     7381 tccgcggtaa ttttcgaaaa ggatcggctc atgcccgtca gcgcgtatgt ttttgcaata
     7441 tcggcgtggc attgcgcaca ccaggcgcca tcgacatagc tgttaggggc cgtttttact
     7501 gaggtgtacg cgccggtgat aggagcggaa gcagtgcgtt gaggcagcct ctgctgggca
     7561 atcaatacgc cgctcaagat gacggttgcg agaatcgagc cgctggtgaa gacgcggatg
     7621 gctgtggctt ttctcatgcg cgcagcagta taaaccgaaa ggagaaaagc gattccagcg
     7681 tactaaacgc gagaggtcct aatttacggt tggctgacga gttggtgtac ctgattgccg
     7741 cttagctcgg ccctaagaac atcgcattcc agattcttat tgcctaggtc gcgtcgcaaa
     7801 cgggaatttt cctgctccaa ttctctcaga cgccgaatgc cactggcggc caggccttgg
     7861 tactgcgttc tccacctgta aaaagtggcc tcgctcactc cgatcctgat gcaaattttc
     7921 cgcaccagaa ctcccgatgc cgcctcattc agcagctcga tgatctcttc cgcctcgaac
     7981 cgactcttcc tcattgcccc atttctccat acgaatccga taaaaaatct attcgttggt
     8041 cgggtcttgc ggggggggac aggccccccc cgcaggacca accgattaga aaaggaactt
     8101 cagtcccaat gacagaatgc gcgcgggctg agtagcagtg aatcgaccga acgttgtgtt
     8161 atttcctcct ccctgcgtga aaggaacatt cgcggtgttt gttgtcaaac cggtgctcag
     8221 cgtcgtgaat tccgtatggt taaaggtgtt ataggattca aaccggaact gcagcttccg
     8281 agcttcattt gtcttgctca tagcgaagtt cttgaaaatc gacatgtcga agttgttagt
     8341 ggagggtccg atgtagttga tgcggccgga attgccaaaa ccgcaatttg ccgcagtgcc
     8401 gtcgcaaata ccttgcccac cattcggcgc agcgaaagca ttgatgttga ggtactgttc
     8461 cttgagcgct ttgccgccgc ccgggggcac gccgtgcttg ttgtagacgc tttggcccgg
     8521 caccaaactg acgcgagtgc cgtagccgcc gccgccgtta tagttgatgc cgctgccagc
     8581 cccgaccgtg cctgcggttc ctgttacata ttcgaaggtt cccgagaaca cccaaccgct
     8641 gatcgcctca ttgaagatac cgtggctgaa gtgcgtttga gggagattgt aggtccagtt
     8701 ggcaacgata ttgtgcttcg gaccgctctg attgtaatag aggtcgcgcc acacgctccg
     8761 aggtgcgatc aggctgggct cactccattg catttccttt gcccaagtcc aagacagatt
     8821 cagagtgacc gtgcggccaa agcgcttgct cacctgcgat tgcagcgagt tgtaattgga
     8881 gttgagattg tcatagatct ggttaacacg cgcatatccc ggcagattcg tattcaagat
     8941 accgagatta ttcgcgtgct ggatcgtgta gccattgttg atcgacggat agttgtagaa
     9001 cacgcttggc aggagtgtgc agccgccctt tgtcgggtcg ccgaaggcaa gggtcacacc
     9061 tggcgcgggc gtgcagccgg atggattggt tgctgagctc ttattcgctg cgcgagcgta
     9121 gctcccatcg gtttcgagcc attggatgcc atagggctca acatcctgcg cgatgcctga
     9181 gttgttgtaa ttgtggcgat tctggatacc gacgtaggtc acatccaaga gaaggccgtg
     9241 cccgaggtcg tgctgaatgc caaggctata gttgtacgat tgtgggtcct tgaagttacg
     9301 aggcagaccg attacgttct gcgggccgat gaagcaatcc gacgtggcag aggtgacaga
     9361 gcacgccgca ttcgggttgg actggttcgc gagcattgta ggcagcgtgc tgttgtaaat
     9421 cgtcgggctg atcggcaact gctgcttgtt agtggacgga atcggctgac tggcgagaag
     9481 ggcaggcgac ggaggcgaaa gcaccaactg accgaccgta tctaccacgc cgaatacgtt
     9541 attagttatg ccaaagccgc cgcgcagagc ggttttgcca ttgccaaaga cgtcccaggc
     9601 gaaaccgaag cgaggcagaa tgttgagtgg tggcgtgttc atcacgggcg tgccgggtgt
     9661 gtaggcgacg atgccctgga acggggtgac tcccgatacc gctattgggg cgaattgccc
     9721 aatagcagac tgggtgaacg ttgttgagcc atagcagccc atacggacgc cacccaccat
     9781 gcagggagcg ataagtccgg gctgagcgga ggcctgatag acgtcgggac ggaagtcgga
     9841 gattaacacg gtgtcgaagc tcgggacgtc atgcgcgaaa cgcatgccgt agtccaatgt
     9901 cacgcggcgg gtaattttcc acgtgtcctg aatgaaccat tcttcaccgt ggaaccgatc
     9961 atagccgttc ggatgcgtgc tggtttcgtt gtagctctga aacacaccat agtaggcgtt
    10021 ggccaaaccc agatttatgt cgttgggatt ggtggaattc ccctgaaagt tcacattgcc
    10081 gttccaaccg cccaaggcac cgccgccggc cgggccattg cgcgagacct tttcaaagta
    10141 gaaaccaccc ttgatggcat gggcaccctt gatccaggtg atgtcgtcct ggaggttgta
    10201 atgagtgtct gtgccgaaga agggatagcg gttgtctcct gacgtgcccg catagctgtt
    10261 tccatagctg gttgtgggta tgtagttaaa tggattggcc gcgagggact gggagttcgc
    10321 tccaaatttg gagttgctcg ggtcaggaaa gattaccgga agaacgctgg gtcccagacc
    10381 gtccagattg cgttcgttcc ggccgaagta accgtaagcg ttcttgttcg tcgtattcgc
    10441 cggaaagacg gtttgtaacg cgcgggtggt gccgacgatg aactcattga caagattcgg
    10501 acgaagcgtc gacacgaggt tcgaagaaac ctcgccgccg tgaatctcgt aattagtcgt
    10561 cagctcgttc cagccattgc ccgcaccaag gaaggtgccg ccgcacgtgc attggtatcc
    10621 cttctccagg tcaaccgtcc agatgttgtt cttcgaaaga ttgtactggc cggtaagaac
    10681 gtaatcgctt cgcgggttct gtgtgacgga gagcagggtg tagttgtagt tgttcgaata
    10741 agcgcttccg agaccagcgc cgcaagccga aaggttgagc gtaggattgg ctgacttggc
    10801 ggcggtaccg tcgccaagtc caccgttata gagatctgag tcgctattgc aggtcggcag
    10861 cgggaagacg ttcaggaggg ggttgaccga acccatagcg cccgcccatg gtgctgcagt
    10921 gatagcgttg gcgccgcagg cgaccgtgag attagcggcc tgggcggggc tgaggtacgg
    10981 gttcgatccg ctagaggttt ggttggctcc gcctgtcggt tcagttcccg ggcagtagac
    11041 ctccgtcccg gcgaaagccg gattgcccag cccgaagatg ttggcaggaa cggagcagcc
    11101 ggcgaggttc ttgccctttg agtccacgca tcccgcgatt gcgccagttt ggcctagtgg
    11161 attcgatgcc acttccgaga aattgcccat gcgctcctct tggctgggca cgagtgcagt
    11221 tgatgttgaa ccggtattgc gccggaggag atctgccgag aagaaaagga agagcttatc
    11281 gcggttctta ttaaagggaa ccttcggcag ccagaatggg ccaccgattg tgccgccggg
    11341 gttatgatat gtgtaactgt cgggatgtgc gcgagtataa gggttggcac tttccttgtt
    11401 gaagtaggtg tttgcgttga agaaccggtt gcgctcgtaa tcgtagagat cgccgtggaa
    11461 ctgattggtg ccggtcttga acatcacatt gatcgagcca ccgttacggg caccgtattc
    11521 ggcgttaaga ttgccggtct ggaccttcat ttcctgaatg gcatccgtac tgggcgcgag
    11581 gtatgcggcg gagctattac ctatgccgtc gtccgaactg gcaataccgt tcagcatgac
    11641 gatgacggca ttattcccgc cgttgatcgt taaaccgtcg gtctggtccc atccgggcgc
    11701 atcgccgccg ccaggtttgt tgtttgtcac cccggggagg agggcgatat aagacatgta
    11761 attgcggccc ttcaccgtga catcggtcat ttgggtcgta ttgacaagag ccgagtgttc
    11821 tgagctgtcc gtctccacgt gagtggcact ggcttgaacg gtgacttccg tgctcacatt
    11881 gccgactgac agtgggatcg tgtgcaaagc cactttttcc gttggcgcga cggcgatgcc
    11941 tttttgcgag taggtctgaa aaccggactg ggcgatacta atgtcatacg tgcccggcac
    12001 gagatcgggg aaaacgaaat cgcccgaact cgaggtctgc accactcttt gctggccggt
    12061 aagagggtag gttagcgtca cctttgctcc aacaatcaca gcgccggtcg ggtcggtcac
    12121 tgttccggcg atctgcccgt taatcgtttg ggccggcaac tcactcgcgc agacccctaa
    12181 cagaagggtt agcaccgcga cgatcgacag cagccggtac ctaccttccg ttctgaaata
    12241 cctatcccca aattttcttc tcatttatcc accccttagc gatgaagaat gtgcgaccca
    12301 agggttttta tgctggcgcg tcccaccccc aagccgacca actcccaatg gctcgggaga
    12361 tttcgccggt cttccaacac gaacgcgcag gcatccgagc gtcaggtcca gaagcgcatc
    12421 gcttcaccct gccctgacaa cctggccctt cttacaacca cccgcctgga gaatgcttgt
    12481 taccaattga aactgtttat ttctgggaat gaggaagtat cttttggccc cggaaacgtc
    12541 aatcacacgc gtcttaattc tttctttaag cgtatatatc tgaatgcaag cagcttaatt
    12601 cg
//
LOCUS       DATNDT010000525        11998 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2446610, whole genome shotgun sequence.
ACCESSION   DATNDT010000525 DATNDT010000000
VERSION     DATNDT010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 11998)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11998)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11998
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2446610"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..931)
                     /locus_tag="VN788_17030"
     CDS             complement(<1..931)
                     /locus_tag="VN788_17030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22285.1"
                     /translation="MIRSVIALLGRRDTPVDGVEDYCTFLSEGLARRGVEMTRVRVNW
                     TEQGWLGALRQLRRDSKEWREHWVLLQYTALGWSRHGFPIWAVIVLGMLRRRGVRTAV
                     VFHEPYRQGERSPRLVDRIRGGCQDWVIRKLYDGCKTPIFVDPVATIRWLPKGDTRSI
                     FVPIGANIPEPAVRSETPTEKLGTTKTVAIFFLTEPPRMQTEVGDIAFAARRAASHIP
                     QMRVIFLGRGTPEAREEIERAFSDTAVEVRNLGLIGAAEISETLTSADAMLCVRGRIT
                     PRRGSVMAGVACGVPIVGYGGAAEATPLAEAGIE"
     gene            1573..2394
                     /gene="kdsA"
                     /locus_tag="VN788_17035"
                     /pseudo
     CDS             1573..2394
                     /gene="kdsA"
                     /locus_tag="VN788_17035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012647357.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
     gene            2487..3497
                     /locus_tag="VN788_17040"
     CDS             2487..3497
                     /locus_tag="VN788_17040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HXU22286.1"
                     /translation="MSPATPRKTAVSPNGRKVSVPTAKRVLQIEAEAISGLIDRLDER
                     FENAVELLYSCEGRVVVAGLGKSGLIGRKIAATFSSTGTPSMFLHAAEALHGDLGMLA
                     SGDALLTISTSGETEELIELVEAARRLGIKIVTLTAKESSTLAIASDIVLDIAVKEEA
                     CSLNLAPTASTAAALAMGDALAIALLERRDFREDDFAALHPRGGLGKKLRRIEMLMHM
                     GDELPRVLPTTKMPDVIYEMSSKGLGLTAVTKPDGHLMGIITDGDLRRVMQRRKQNVL
                     ELAAIDCMTKNPVTLPRTELAATALRLMEEKKITSVVVVDAAGRLEGIVHIHDLWTLQ
                     LF"
     gene            3518..4078
                     /locus_tag="VN788_17045"
     CDS             3518..4078
                     /locus_tag="VN788_17045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HXU22287.1"
                     /translation="MPIREIKFPAKVMRRAKKIRVLLMDVDGTMTPGVVCLQAFPDGS
                     VAEMKVFNAHDGAGIKLASIMGIRTGLITGRDSPATARRAREASMEFVIQGQPKKLES
                     YKAILIRAGVTDEEVAYVGDDLPDLPLLERAGLAIAVGDAATEVKRVAHYVTTVDGGK
                     GAIREVVELILKSQGKWKKAIPLAIA"
     gene            4252..4821
                     /locus_tag="VN788_17050"
     CDS             4252..4821
                     /locus_tag="VN788_17050"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HXU22288.1"
                     /translation="MATPKETALFAGMRLSLCGLLSAAAFLVAGALAPAVSSRALPGN
                     DSPGRLDSPESQMLYLTNQDRTARENWEETQGRAHPLQWDDRLAAVARAHSEDMARQG
                     YLTHTGSDGSMPFQRVSYMGIQWLSTGENVARARDTAEAEALFMDEPKFQQNHRANIL
                     NPNYTIVGIGVARDSEGALYITEEFAQLR"
     gene            complement(4869..7145)
                     /locus_tag="VN788_17055"
     CDS             complement(4869..7145)
                     /locus_tag="VN788_17055"
                     /inference="COORDINATES: protein motif:HMM:NF017390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HXU22289.1"
                     /translation="MISASVAHACSTFLAHLIAPAARSLCLGCFAALAMGALRVKSVS
                     ARLAVWRWVLYAALAMPLLGLILPPLSFELPAAVARLIPQRATVANAVRPSEETAAPG
                     GFAPEAFPVSPLRMDIEIPLPNSDSDVRPISAAQTSHSDVRPADGASSAKAAPIVGES
                     KASPVIVAKNRDASFPWIVFFAATYISITLIFLVRLLLGFIFSRRLVRGARPIAESEA
                     TDLLNLREREDHLAVLLCLAESELISVPITLGIVRPIILLPVGWREWSSAALRAVLAH
                     ERSHVIRRDALMQRLSLLHRAIFWFSPLSWWLHRCLADAAEEASDEAALLSGADRAQY
                     AETLLDFFVALQRNPRRVNWQGVSMANAGQAAEKRVDRILAWEGGVSMHINRSLSIIL
                     LLLGITVVFFAATARPSIAHAQDRAIVPIPVPITVPAPTKPSATDNLEDEPPRPMAVA
                     SQQAPAVVQPTPEPAFPPAPAPPAPPPSEAGPNLFAVPPTVELHFKDVISEDQIRKIY
                     RDAENAVSRAGFSQIPVQEAMEIQLQQFQHDGVFRAGWPGFGGGFGGRYVIVSGDSPI
                     LMSGNSEDVEHATSLRNKIKGDYIWFQHDEKNYVIRDQATVQRAKDLFKPEEELGQKQ
                     DALGKQQEALGDQQRELAKKMEAVHVQIPDMTGDIQKLEAQMKQLSAGGTQQQLGDLQ
                     RQIGELQHKIGESQYQAGDQQRQIGEQMRELGRQQGEIGRQQGELGRQQAEASQQANQ
                     QMKQLLDDAVTRGTAQPE"
     gene            complement(7142..7591)
                     /locus_tag="VN788_17060"
     CDS             complement(7142..7591)
                     /locus_tag="VN788_17060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HXU22290.1"
                     /translation="MTNTRRIRPRNLGDVEQAVMDYVWSRGPCTADACREALAGSRPM
                     KDSTIRTVLRRLEAKGYVAHKVEGRTFLYRAAEARENVAARAVKHIIDRFCDGSAEQL
                     VMGMVDSNVLDRKDLERMARKIAQSRQGRSKSSARSDARPSGKDDKP"
     gene            complement(7722..9902)
                     /gene="glgX"
                     /locus_tag="VN788_17065"
     CDS             complement(7722..9902)
                     /gene="glgX"
                     /locus_tag="VN788_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010584599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HXU22291.1"
                     /translation="MGEPSIKVRTGAPYPLGAIWDGAGVNFALFSEHAAKVELCLFDS
                     AGDHKESQRIELKEQTDMVWHGYLPWILPGQVYGYRVHGPYDPAAGHRFNAAKVVLDP
                     YAKAIARTVNWAPEMYAYRQDGEDADLVMDDRDNAAFAPLAAVVDTAFTWGNDRQPRT
                     PWHNTVIYELHVKGFTRLRSDLPEKMRGTYSGLGSEPSIRHLLDLGVTAVELMPVHHH
                     SVDNHLVDLGLTNYWGYNTLAFFAPDIRYSMTQTPLESVRAFKTMVKNLHSAGLEVIL
                     DVVYNHTAEGNHLGPTLSLRGVDNAAYYRLEQDNQRFYTDYTGCGNTLNVRHPRVLQL
                     IMDSLRYWVMEMHVDGFRFDLASALARELHAVDKLGAFFDIIHQDPVLSQVKLIAEPW
                     DIGEGGYQVGNFPVGWTEWNGKYRDAMRRFWKGDGGVVSELATRLAGSSDLYSHSGRR
                     PYASINFVCAHDGFTLNDLVSYNEKHNEANLENNQDGDNNNLSWNCGAEGPTNDPQIL
                     SLREQQKRNFLATLLLSQGVPMICGGDEFSRTQQGNNNAYCQDNELSWLDWNWTAKQK
                     DLVEFTRYVIGLRKEQAVLRRRKFFQGRSIRGADVKDIAWYEPRGNEMTDKAWGDPVA
                     RCLGVLLNGNTLGEIDERGEPVVGDTLFLMFNAYHEPILFRLPVKQPGERWERLLDTA
                     DEHIKTGLLLRDHTYKIHGRSVVVVRLKAADAPKPEATTAGEPA"
     gene            10182..11990
                     /locus_tag="VN788_17070"
     CDS             10182..11990
                     /locus_tag="VN788_17070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HXU22292.1"
                     /translation="MSDNPTKPSASKKLKLANLLRSHKRGLTIAFVAVLGETLTDVLQ
                     PWPIKIIVDNIIQNKKLPKWLGGIVSGYFGHNKLSILNFAVAAVAAIAILGAVSSYIE
                     KYMTTSVGQWIAHDLRRTLYNHIQRLSLAEHDETRTGDLISRVTDDIDAVQSFINSAL
                     LGIVVDVLTLVGMMGVMFYVNWKFTLIALSVVPALFAVVYYLTRRIKRASRAVRKSES
                     ELTSIVEEALTSVRVVKAFAREDYEMQKFESESLENVETALRARSLKATLAPLVEVIV
                     AIGTCLVLWDGARLALDGRISAGVLIVFLLYLGNMYKPMRDLSKQTDTVSKALVGYER
                     IQEVLEIESQVRDLPRARRAPRFKGTIEFDHVHFSYHGETPVLKDVSFRIEAGQVAAL
                     VGPSGQGKTTISSLIPRFYDPTSGTVKIDGSDIRDFKLRSLREQMSFVLQETLLFRAT
                     VWDNIAYGKPDAKPKAIIRAAQLANAEEFIETLPQGYSTVIGERGASLSGGQRQRIAI
                     ARAIIRNTPILIFDEPTSGLDAAAEQTVMEALNRLMEGRTTVLVAHHLSTIRRAEVIF
                     VIGDAEIVEHGSHEELLAANGPYAELYRIQTESVTK"
BASE COUNT         2589 a         3529 c         3364 g         2516 t
ORIGIN      
        1 actcgatgcc ggcctccgcc agcggcgtag cctcggccgc tcccccataa ccaacaatag
       61 gcactccgca cgcaacgccg gccatcacgc ttccccggcg cggcgtgatc ctaccgcgga
      121 cgcaaagcat cgcgtcggcg ctcgttaaag tttcagaaat ttctgcggcg cctatcaatc
      181 caaggttacg gacttccacc gccgtatcgc tgaaggcacg ttcaatttct tctcgggctt
      241 caggagttcc tcgtccaagg aaaataaccc gcatctgcgg aatgtggctt gcggcgcgac
      301 gagctgcaaa agcaatgtca ccgacctcgg tttgcatcct gggaggttcg gtgaggaaga
      361 aaatcgctac cgtcttcgtc gtgccgagtt tttcggtcgg tgtttccgaa cgcacggctg
      421 gctcgggaat attggccccg attgggacaa aaatcgatct ggtatcacct ttaggcagcc
      481 acctgattgt ggcaacggga tcaacaaaga tgggagtttt gcaaccatcg tagagcttgc
      541 gaatcaccca atcctgacat ccgccgcgga ttcgatcgac caagcgcggc gaacgctcgc
      601 cctgccggta gggctcgtga aataccacgg cggtccgcac gccgcgtcgt cgcagcattc
      661 ccagcacaat gaccgcccaa atcggaaatc cgtgcctcga ccatcccagg gccgtatatt
      721 gcagcagtac ccaatgctcg cgccattcct tcgagtcgcg ccgcagttgg cgcaaagctc
      781 cgagccaccc ttgctccgtc cagttcacgc gaacgcgcgt catttctacg ccgcggcgcg
      841 ccagaccctc actcaaaaaa gtgcagtagt cctccactcc atcaactgga gtatctcgtc
      901 gccccagcag cgcaatcacg gacctgatca taccaacttc cgctcgttcc ttaggccaga
      961 ccaatgacgc cgatcacctc ccgaattcag agggacccaa tcaccagcta gtagcgtgac
     1021 gccatcgctc cgtaggataa attcgctagg gatccaaaat tctttacacc accatgtgct
     1081 tgaaacggcg attttcgatg atagcctcac attgaggctg cgtagccgcc ttggtgaaag
     1141 gagatgatgg cgatgtggga ctttcaggca agaggtcgcc cccgctatct gtggcgaagt
     1201 ggcgccacgc ccactcgcga tagaaagctc tgtgccccgg gcctgctgtc aatccggagc
     1261 cgcgtctgcg agagtcgccc gaagagcgaa actgccgcca gccgcctcaa attctattcg
     1321 acacttctgc cgagtagaaa cgcttgtaac tccctgaaaa taaacggtcg gtgtcaatcc
     1381 tacccgtcac gaaaatggag agcgcgtttg cggtcggatg gccataacca cgcagatggc
     1441 gtaggcgaat tccagattga aaacggaccg gaacgcgctt acgctcttgg tcttagtgat
     1501 tctgcaaacc caattgctag atcgatctgc agggacggcg tgcagcattc gcaagccgtc
     1561 aggcgcactt tgatggagcc tccccgcagc ttcggagtcg ggaaaatccg catcggcggc
     1621 aacgcgccat tgtttctcat cgccggcccc tgcgtaattg aaagcgaagc gcatgccacc
     1681 tcaatcgcca ttcgcctggc cgaaattgcc ggctccgtaa gtatcccgct gattttcaag
     1741 gcttcttacg acaaggccaa tcgctcatcc atcggatctt tccgggggcc gggtcttgaa
     1801 gccggccttc gcgtgcttgc caaaatcaag aagcgcaccg gactgcccat ccttacggac
     1861 gtgcacgaag tacagcacgt tggccccgcc gctgaagttt gtgacgtgct gcaaatcccg
     1921 gcatttctct cgcgtcaaac ggacctgctg gtcgccatcg gccggtccgg ccgcgtcgcc
     1981 aacatcaaga aaggacagtt cctttcgccc tgggatgttg cgcatgccgc ggaaaaaatt
     2041 gcaagcactg gcaatcaaaa aatcattctt acggaaagag gcgtatcctt cggctaccag
     2101 aatcttgtgg tagacatgcg atcatttccg attatgcgca aactgggata tcccgtggtc
     2161 ttcgatgtga cgcacgcagt gcaactgccc ggcggagaag ggaattcgag cggcggccaa
     2221 cccgagttca tcgagcccct cgcgcgtgcg ggaactgccg ccggcattga cggtctgttt
     2281 ctcgaagtgc atgacaatcc cgcgaaggct ctctcggacg gcaccaacgc gctcgcactg
     2341 gaagacctcc ggccactcct ggaaaaagtg tcgggcatcg ccgcgctcac tcgcgaatgg
     2401 tacggcaatg gcgggaatcc cgaaaagcag cgctcttcac cgaaggcggc acaacgcaaa
     2461 ccggagcgca gaaagtggcg cgcccgatga gcccggccac gcctcggaaa accgcagtct
     2521 cgccgaacgg aagaaaagtg tcggtgccga ccgcgaagcg cgtcctccag atcgaagccg
     2581 aggccattag tggtctgatc gatcgtctgg atgagcgctt cgaaaatgcg gtggagcttc
     2641 tttactcctg cgaaggccgc gtcgttgtgg ccggcctcgg caaatccgga ttgattggac
     2701 gaaaaatcgc ggccactttt tccagcaccg gcacgccgtc aatgtttctg catgccgcgg
     2761 aagctctcca cggcgatctt ggcatgctcg cttctggcga tgccctgttg acgatttcga
     2821 cgagcggtga aaccgaggaa ctgattgaac tggtggaagc agccaggcgt ctcggcataa
     2881 aaattgtaac gctcacggcg aaagaatcct cgacgctggc gatcgcaagt gacatcgtgc
     2941 tagacatcgc tgtcaaagaa gaagcctgct cactgaatct tgcgcccacg gcctccacag
     3001 ccgcagcact ggctatgggc gacgcgctcg caattgcttt gcttgaacgg cgcgattttc
     3061 gcgaagacga tttcgccgcc ctgcatcccc gtggaggcct ggggaaaaaa ctgcggcgca
     3121 tcgagatgct gatgcacatg ggcgacgaat tgccgcgcgt gctacccacc accaagatgc
     3181 ccgacgtgat ctacgaaatg agcagcaagg ggctgggcct caccgcggtg accaagccgg
     3241 acggccatct aatgggaatt atcaccgatg gcgaccttcg ccgcgtgatg cagcggcgca
     3301 agcaaaacgt tctggaactc gccgccatcg attgcatgac caaaaaccca gtcacactgc
     3361 cgcgcaccga gcttgcggcc acggcacttc ggctcatgga ggagaaaaag attacctcag
     3421 ttgtggtcgt cgatgccgcc ggccgcctcg agggcatcgt ccacattcac gatctttgga
     3481 cactgcaatt gttttagcgg tttagccgga aatccaaatg ccgattcgcg aaatcaaatt
     3541 tcccgcaaaa gtgatgcggc gcgcgaagaa aatccgcgtt ctgctgatgg atgtggacgg
     3601 cacaatgact cctggcgtcg tctgcctgca ggcttttccc gatggcagcg tggccgagat
     3661 gaaagttttc aatgcccatg acggtgcggg tatcaagctc gcgagcatca tgggtattcg
     3721 caccggtttg atcaccgggc gcgattcgcc cgccacagca cggcgcgccc gcgaggcgtc
     3781 catggaattc gtgattcagg gccagccgaa aaaactcgaa tcgtacaaag cgattctgat
     3841 tcgtgctggt gttaccgacg aagaggtcgc ctatgtaggt gacgatctgc cggatcttcc
     3901 actgctggaa cgcgccggcc tggccatagc agtgggtgac gcagcaaccg aagtgaagcg
     3961 cgtggctcac tacgtcacca cggtcgatgg cggcaaggga gccatccgag aagtagtcga
     4021 gctgattctg aaatcgcaag gaaaatggaa aaaggcaatt cccctcgcca tcgcctgaac
     4081 gcgctccctg tcgccggggc gtagtcccaa ttcccgcttt tgactcgaat cgcgtgaggc
     4141 gaaatttagg cgatagccgt gtgcctgtac gtaccagaac taaaacggta ctttcggcca
     4201 gtgtctcgcc aaactccgga agcatagcct tatggagtga gcgagaaact gatggccact
     4261 ccgaaggaaa ccgcgctttt tgctggaatg cgtttatcac tttgcggcct gctctccgca
     4321 gcggcgttct tagtggcggg cgctctcgct cctgcggtat cttcgcgggc tttgcccggc
     4381 aatgattccc ccggccggct cgattcacct gaatcgcaga tgctctacct gactaatcag
     4441 gacagaacag ctcgtgaaaa ttgggaggaa actcagggcc gcgcacatcc gctccagtgg
     4501 gacgatcggc tggcggcagt ggccagagcg cactcggagg atatggcccg acagggatat
     4561 ttaacccaca cagggtcgga cgggagtatg ccctttcaac gcgtttccta catgggcatt
     4621 cagtggcttt ctaccggaga gaacgtggcc agagctcgcg atacggcaga agccgaggcc
     4681 ctattcatgg acgaaccgaa attccagcaa aaccaccgag ccaatatcct caatccgaat
     4741 tacaccatcg tcggcatcgg cgtcgccaga gattcggaag gagctttgta cattaccgaa
     4801 gaatttgcgc aactccgcta gctgattcct ggcatttctc caccagtgca accgaaaaat
     4861 tgcgacgttt attccggctg ggccgtgccg cgagtgacgg cgtcatccag aagctgcttc
     4921 atctgctgat tggcctgttg ggacgcttcc gcctgctgcc gtcccagttc gccttgttgc
     4981 cgcccgattt caccttgctg tcgcccgagc tctcgcatct gctcgcctat ctgccgctgc
     5041 tgatcgccgg cttgatattg agattctccg attttgtgtt ggagttcccc gatttgacgt
     5101 tgtagatcgc ccaactgttg ctgcgtgccg ccggcgctga gctgcttcat ctgggcttcc
     5161 aacttttgga tgtctccagt catgtccgga atttgcacgt ggaccgcttc cattttcttg
     5221 gcgagctccc tttgctgatc tcccaacgcc tcctgctgtt ttccgagagc atcttgtttc
     5281 tgaccgagtt cctcttcagg cttgaacaaa tcctttgcgc gctggacagt ggcctggtcg
     5341 cgaatcacat agtttttttc gtcatgctgg aaccagatgt aatcgccctt gattttgttg
     5401 cggagcgaag tggcgtgctc gacatcctcc gaattgccgg acatgaggat tggagaatct
     5461 cccgacacga tcacgtagcg tccgccaaag cctccgccga acccaggcca tccggcccgg
     5521 aatacgccgt cgtgttgaaa ttgttggagc tgaatctcca tggcttcttg aaccgggatt
     5581 tgcgaaaacc cagcacgcga aaccgcgttc tctgcgtccc ggtaaatctt acgaatttgg
     5641 tcttcactga tgacgtcttt gaagtgaagt tcgacggtcg gcggcactgc gaaaaggttt
     5701 ggtccagcct cggagggcgg aggcgccggc ggcgcagggg caggaggaaa tgccggttca
     5761 ggtgtgggct gaactacagc aggcgcctgc tgagaagcca cagccatggg acgcggcggt
     5821 tcgtcttcca aattatctgt cgcgctcggc ttcgttggag ccggcaccgt aatgggcacg
     5881 ggaatcggaa cgattgccct atcttgcgca tgagcaatcg atggccgcgc ggtggctgcg
     5941 aaaaatacaa cggtgatgcc aagcaacaac agaataattg acagtgacct attgatgtgc
     6001 attgaaacgc ctccctccca tgcaagtatc cgatccactc gcttttccgc cgcttgccct
     6061 gcgttagcca ttgagacacc ctgccagttc acgcgccgcg gattccgttg aagcgcgacg
     6121 aaaaagtcga gcagcgtttc ggcgtactgc gcgcggtccg cgccggaaag cagcgcggct
     6181 tcgtcgcttg cttcttccgc ggcatcggcc aggcaacggt gtagccacca gctcaaaggg
     6241 ctgaaccaaa aaatcgcgcg gtgcaaaagt gaaaggcgct gcataagcgc gtctcggcga
     6301 atcacgtgcg accgttcgtg cgccagtacg gcacgaagtg cagcggaact ccactctcgc
     6361 catccgactg gaagtaaaat aatcgggcgc acaattccca gagtaatcgg cacggaaatc
     6421 aattcggatt ccgcaaggca tagcagaacc gcaaggtgat cctcgcgctc acgcaggttt
     6481 aggagatccg tggcctccga ttcggcaatc ggtcgagcgc cgcgaacgag tcgtcggctg
     6541 aagatgaacc cgagcagcag ccgcacgagg aaaataagag tgattgaaat ataggtcgcg
     6601 gcgaaaaaca caatccatgg aaacgacgcg tcacgatttt tcgctacgat caccggagaa
     6661 gctttcgatt cgccaacaat tggcgccgcc ttcgccgagg aggccccatc ggcaggtcga
     6721 acgtctgagt gcgaagtttg cgcggcgcta atcggccgga catcggaatc gctattgggc
     6781 aaagggattt caatgtccat gcgaagtgga ctaacgggaa acgcctcagg tgcgaaacct
     6841 ccaggcgcgg cagtctcttc gctcgggcga acggcgttcg cgaccgtagc tctctgcgga
     6901 atgagccgcg ccacggctgc cggaagctca aaagatagcg gaggcaagat caatcccagc
     6961 agcggcatgg ctagcgccgc atagagcacc cacctccaaa cagccagacg cgccgaaaca
     7021 cttttcacgc gtagggcgcc catcgccagc gccgcgaaac atcccaggca cagcgagcgc
     7081 gcggccggcg caatcagatg cgcgagaaac gtcgaacacg cgtgagccac cgaggcggaa
     7141 atcatggctt gtcatccttt ccagacggtc gcgcgtcaga cctggctgac gacttgctcc
     7201 ggccctggcg gctctgagca atcttgcgcg ccatgcgttc gagatctttg cgatccaaga
     7261 cattgctatc gaccatgccc atgacgagct gttcggcgga gccatcgcaa aatcggtcaa
     7321 tgatatgttt cacggcgcgc gccgcaacgt tttcgcgcgc ttccgcggcg cggtaaagga
     7381 aagtgcgacc ttcgactttg tgggcgacat agcccttggc ctcgagccgg cgcagaactg
     7441 tgcggatggt cgagtccttc atcggacggc tgcccgccag cgcttcccgg caggcgtccg
     7501 cggtgcaagg gccgcgcgac cagacgtaat ccatcaccgc ctgctcgacg tcgccaagat
     7561 tcctgggccg gatgcgccgt gtgttagtca ttgtagcgtt acagtgtgta acacttgaga
     7621 agccagcgtg tcaagagaaa atttcgcggc ggggccgcgc ccaataatcg gggagagata
     7681 ggttgccgcg aaaaagtcta tacctggcgg agccagcgcg cttatgccgg ctcacctgcc
     7741 gtagtcgcct cgggtttggg tgcatcagcg gccttcagac gcacaacaac aaccgaccgt
     7801 ccgtgtatct tgtaggtatg atcgcgaagc aataaacccg tctttatgtg ctcgtcggcg
     7861 gtatccagca gacgctccca cctttcgccg ggctgcttta ccggcaggcg gaagagaatc
     7921 ggttcgtggt aagcgttgaa cataagaaaa agcgtgtctc cgaccaccgg ctctccgcgc
     7981 tcgtcgattt cgcccagagt attcccattc aacaggacgc ccagacatcg cgcaacgggg
     8041 tcgccccatg ccttgtccgt catttcgttg ccccgcggtt cataccaggc gatatctttg
     8101 acgtcggccc cgcggatgct gcgtccttgg aaaaatttcc ttcgacgtag caccgcctgt
     8161 tcctttcgca gcccgatgac gtagcgagta aactccacca agtctttttg cttcgcagtc
     8221 cagttccaat ccagccagct cagttcattg tcctggcagt acgcattgtt atttccctgc
     8281 tgcgtgcggc tgaactcatc tccgccgcag atcatgggca ctccctggga caacaagagc
     8341 gtggctagaa agttgcgttt ctgttgttcg cgcaacgaaa gaatctgggg atcgttcgtg
     8401 ggcccttcgg cgccgcagtt ccagctcaaa ttattgttgt cgccatcctg attattctcc
     8461 aggttggcct cattgtgttt ctcgttgtag ctgactagat cgttgagggt gaacccatcg
     8521 tgggcgcaga cgaaattgat gctggcgtac ggccggcggc cgctgtggct gtaaagatca
     8581 ctgctgccgg caagccgtgt agccaattcg gaaacgacgc cgccatctcc cttccagaac
     8641 ctgcgcatcg cgtcgcgata ttttccgttc cattccgtcc accctacggg aaagtttccc
     8701 acttgatagc cgccctctcc aatgtcccag ggctcggcaa tcagttttac ctgagacaac
     8761 accgggtcct ggtgaatgat gtcgaaaaag gctcctagtt tgtccacggc atggagctcg
     8821 cgcgccaaag cgctcgccag atcgaagcgg aaaccatcga cgtgcatctc catcacccaa
     8881 tacctcaggc tatccatgat cagttggagg actctggggt gccgcacatt gagggtattg
     8941 ccacagcccg tgtaatccgt gtagaagcgc tgattgtctt gctccagccg atagtatgca
     9001 gcgttgtcta cgccgcgcag ggaaagcgtg gggccaaggt ggttgccctc cgccgtgtgg
     9061 ttatagacga catcgagaat cacttccagg ccggcggaat gcagattttt caccatcgtt
     9121 ttgaaagctc gcacagattc cagcggagtc tgcgtcatcg agtagcggat gtcaggcgca
     9181 aaaaatgcaa gagtgttgta gccccaataa tttgtaaggc ccagatctac gagatggttg
     9241 tcaacggagt ggtgatgaac cggcatcaac tcaaccgccg taacccccag atccagcagg
     9301 tggcgtattg agggttccga gcccaatccc gagtatgttc cgcgcatttt ttcaggaaga
     9361 tccgagcgca ggcgagtgaa gcctttgacg tgcagctcgt agatcaccgt gttgtgccac
     9421 ggcgtacgag gctggcgatc gttcccccat gtgaaagcgg tatccacaac cgcggccaga
     9481 ggggcgaaag ctgcattgtc gcggtcgtcc attaccaagt ccgcgtcttc gccgtcttgt
     9541 cggtacgcgt acatttcagg cgcccaattt acggttcgtg cgatcgcttt ggcgtatggg
     9601 tccagcacaa ccttggcggc attgaatcga tgtcctgccg ctggatcgta aggcccatga
     9661 acgcggtatc cgtagacctg gccgggcaga atccatggca ggtagccgtg ccataccatg
     9721 tctgtctgtt cttttaactc gatacgctgc gattccttgt gatcgccagc ggaatcgaaa
     9781 aggcaaagct ccactttggc ggcgtgctct gaaaaaagcg cgaagttgac tcccgcccca
     9841 tcccagatcg cgcccaaagg gtagggcgct cccgtgcgta ccttgatgct tggctcgccc
     9901 atgcttttcc tcccgctagt tcgattcgca aatctcacac gatggagcaa tcattcagcg
     9961 cgtcctcagg acagcgatag aatagaattg tccggctcac atctctttca atttaagggg
    10021 tttcctgtat tgcgctttgt ttgtctccct acgatgatgg ctgcggaagc gatcacccgt
    10081 tcggcggtga cggtgaccgc gcgacgccgc tggaatcccc ccagaagtgg ccgttggtgt
    10141 gagagtctac tggatacgct tgtcgaggcc agccagagac tatgagcgac aatccgacga
    10201 agccttccgc atcgaaaaag ctgaaactag caaacctgct ccgttctcat aagaggggct
    10261 tgacgatcgc attcgtcgcg gtgctgggtg aaaccctcac cgacgtcctt caaccgtggc
    10321 ccatcaagat catcgtcgat aacatcatcc aaaacaagaa gctgcccaaa tggctgggtg
    10381 gaatcgtttc cggatatttc ggccacaaca aactctcgat tctgaatttt gctgtcgctg
    10441 cggtagcggc gatcgctatt ctcggcgccg tcagttccta catcgaaaaa tatatgacca
    10501 cgagcgtggg tcagtggatc gcccacgact tgcggcgcac gctgtacaac cacattcaac
    10561 gcctctcgct cgccgagcac gatgaaacac gcactggcga tctgatatcg cgcgtgaccg
    10621 atgacatcga cgcggtccaa agttttatca actccgcgct cctgggaatt gtggtcgatg
    10681 tgctgacgct ggttggcatg atgggtgtca tgttttatgt caactggaag ttcaccctca
    10741 tcgcattgtc ggttgtgccc gcattgttcg ccgtcgtgta ttacctcacg cgccggatca
    10801 agcgcgcctc tcgcgccgtt cggaaaagcg aaagcgagct tacttcgatt gtggaagagg
    10861 ccttgacctc ggtccgcgtc gtgaaggcat tcgccaggga agattacgag atgcaaaagt
    10921 ttgaatcgga aagtttggag aatgtggaga cggcattgcg ggcgcggagt ctcaaagcca
    10981 cgctcgcgcc tctcgtggaa gtgattgtgg cgattggaac ctgcctcgtc ctttgggacg
    11041 gtgcgagatt agccctcgac ggccggatca gcgccggggt tctgatcgtt ttcttgttgt
    11101 atctcggcaa tatgtataag cccatgcgcg atctatccaa gcagacggat accgtttcga
    11161 aagcgctcgt aggctacgag cgtattcagg aggtactgga gatcgaaagc caggttcgcg
    11221 atctgccccg cgcgagacgc gcaccgcgct tcaaggggac catcgagttc gaccacgttc
    11281 acttcagcta ccacggcgag actccggtct taaaggatgt cagctttcgc atcgaggccg
    11341 ggcaagttgc cgcactggtg gggccgtccg gccagggaaa gaccaccatt agcagcctca
    11401 ttccccggtt ttacgatcca acttctggaa ccgtaaaaat cgacgggagc gatattcgcg
    11461 atttcaagct gcgttccttg cgcgaacaaa tgagcttcgt gttgcaagag acactgctat
    11521 tccgcgccac cgtttgggac aatatcgcct atggaaaacc cgacgccaag ccgaaagcga
    11581 tcattcgcgc cgcacagctt gcgaatgcag aagaattcat cgagacgctg ccccagggat
    11641 attccacagt gatcggcgaa cgcggcgcat cgctctcggg cggtcaaagg cagcgcatcg
    11701 ccatcgcgcg ggccattatt cgcaatactc caatcctgat ttttgacgag cccacctccg
    11761 gactggatgc ggccgccgag caaaccgtga tggaggccct gaaccggctc atggagggca
    11821 gaaccaccgt gttggttgcg caccatctca gcaccatccg ccgcgcggaa gtgatttttg
    11881 tgatcgggga tgcggagata gttgagcacg gatcgcacga ggaactcctt gcagcaaacg
    11941 gaccgtatgc cgagttgtac aggatacaga ccgagagtgt tacaaaatga atagatgg
//
LOCUS       DATNDT010000526         3947 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3058172, whole genome shotgun sequence.
ACCESSION   DATNDT010000526 DATNDT010000000
VERSION     DATNDT010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3058172"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(451..825)
                     /locus_tag="VN788_17075"
     CDS             complement(451..825)
                     /locus_tag="VN788_17075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HXU22293.1"
                     /translation="MNPATYLAFADAALAAHLLYIAWVILGAFFTRRRPRLAALHIAS
                     IIWGIITETTSAPCPLTIAENWCEARAGVAPYHGPFLLHYLDATVYPNVSASLLVAVA
                     VTVCAANLFVYARRAARSRRRK"
     gene            complement(876..2291)
                     /locus_tag="VN788_17080"
     CDS             complement(876..2291)
                     /locus_tag="VN788_17080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HXU22294.1"
                     /translation="MGGAVGLWSSSLLLQWLSAWRPVPRYPVQAAVSPDANVYIFALL
                     IALVSGFLFGAVPVRQVLQTNPYGVIKSGSTATPGRRLTARDLLLTVQIALCAVLITS
                     SFVAVRGLLRSMHTGLGVDPRNVMLVDVDVNTAGYRGAAVPPVQKRIIDAVHTIPGVQ
                     AVAWTNVPQLTLDCCSSVLVFSDQTANPTPANSVLRATTFKVSREYFRATRTALLAGR
                     EFTDHDDATSSRVAVVNERFARYVFGSVAAALGRDYKLADATRIQVVGVVADGKYGSA
                     TEAPQAAMFFPILQSPSDEAWLIVQSPDDSQQLAASIRRKLTGVDPSLVSFFQTWSGG
                     LDGSMFGPRMAAISLGVLGAMGALLSITGIFGIAAYAVSKRKRDLGIRIALGAQRKEV
                     LRAALGRAVKLLAFGSLAGLLLGILASRVLASIVYQATPRDPLVLAGVVLAMALVGLV
                     ATWIPAHRALSIDPVMLLREE"
     gene            complement(2276..3517)
                     /locus_tag="VN788_17085"
     CDS             complement(2276..3517)
                     /locus_tag="VN788_17085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HXU22295.1"
                     /translation="MFPRIFRTRKASDFSEEIAAHIAAETERLREQGLSEEEAHNHAH
                     CTFGNVTKTQERFYESHHWLFWDNLWRDIRYSLHLLRKSPSFTLAAILTLGMAITANV
                     VAFSILNALFLRPLNVADEQSLYIVEHTPDTTRFQSYPDYLDLRDRNRSFDGLATYGV
                     VGVGLATGQYGQSPSLTWGWKVSGNYFDELRLTPYLGRFFHSSDERGPNSAPYLVLSY
                     SYWHNNFKDDPGVIGRTVQLNKHPFTIIGVTPPDFHGTVPFVAACFYVPLINQEQVNG
                     VNNLNARGVGSIQVIVGHLKHGVTPAQATADVNSIGAYLEKTYPKDDKKMVFAIEHLG
                     AFSSEDNTALATKAFLAALMLLAGLILLAACANLGSPFSARAAERSREIALRLALGST
                     RARILRQLFTEAISSPSWEAL"
     gene            complement(3717..>3947)
                     /locus_tag="VN788_17090"
     CDS             complement(3717..>3947)
                     /locus_tag="VN788_17090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXU22296.1"
                     /translation="QTSDEELLVEHGALYPALQRIEERGWISAKWGTSSNNRKARFYS
                     LTRAGRAQLAKETTKWKRFANAIGRILESEQA"
BASE COUNT          836 a         1013 c         1330 g          768 t
ORIGIN      
        1 gcagggcgtt tttgtggccg accgttatgg cgagctttac gcgagatgga tcatcgagaa
       61 agaaggccaa atgccggcca tttctgagct cctgaaggcc ctcgaccaga tcgaaatcgc
      121 ctgcgaagag tgcaacccca ctacctggcc cctcgacggt tagccttctt caattgccca
      181 tctgaaaaat tacgataatt gccgtgtcga tttacactgg ctggccgttc agcctatatg
      241 gttttgtact gccattgacg aacgcctgct gtagctgact tcgcaaagtc gcaggaacct
      301 ggcattacct gcatgtcgtt cagctttcca tccgttccga ttattgcatg caggaggaat
      361 ttgccccgat ataccccggc tcggctcgac acggtgactc gcgatgggcg tggtaatccc
      421 cgcccctacg atggctggcg gacggcacgc tcatttgcgg cggcgggagc gggcggcgcg
      481 gcgagcgtag acgaacaggt tcgcggcgca gacggttacg gcgacggcga cgaggagaga
      541 ggcggagacg tttgggtaaa cggttgcgtc gagatagtgg aggagaaatg ggccgtggta
      601 gggcgcgacg ccggcgcggg cttcgcacca gttctcggcg atggtgagcg ggcagggcgc
      661 agaagtggtt tcggtgatga tgccccagat gatggaggca atgtggagag cggcaaggcg
      721 gggacgtcga cgggtgaaga acgcgccgag aatcacccag gcgatgtaca ggagatgcgc
      781 ggcgagcgcg gcatccgcga atgccaggta tgttgcgggg ttcatgccag cgtgaattat
      841 atgcgcgatg attgtatgcg ctgaggatgt tgacgctact cttcgcgcag cagcatgacg
      901 ggatcgatgg agagagcacg gtgcgcggga atccaggtgg ctacgaggcc aacgagcgcc
      961 atggccagca cgacaccggc caacaccagg ggatcacgcg gagtggcctg gtagacgatc
     1021 gaggccaata cgcgcgaggc gaggattccg aggagcaatc ccgcgaggga gccgaacgcc
     1081 agcagcttta ctgcgcgtcc gagggcggct cgcaaaactt ctttgcgctg ggcgccgaga
     1141 gcgatgcgaa tgcccaaatc gcgcttgcgt ttgctgacgg cgtatgcggc gatgccgaag
     1201 attccggtaa tggagagaag cgcgcccatt gctcccagca cgccaagtga gattgcggcc
     1261 attcgcgggc cgaacatgct gccgtccagg cctccgctcc aggtttgaaa aaaggacacg
     1321 aggcttgggt ccacgccggt gagcttgcgg cgaatggatg cggcgagctg ctgcgaatcg
     1381 tccggcgatt gcacgatgag ccaggcttcg tctgaaggcg attgcaggat gggaaaaaac
     1441 atggctgctt gcggagcttc ggtggcgctg ccgtattttc cgtcggcgac aacgcccacg
     1501 acctgaatgc gcgtggcgtc agcgagtttg tagtcgcggc cgagtgcggc ggcgacggaa
     1561 ccgaaaacgt agcgcgcgaa acgctcgttc acaacggcga cgcgcgaact cgtggcgtcg
     1621 tcatggtcgg tgaattctct gcccgcaagg agagcagtgc gcgtggcgcg aaagtattcg
     1681 cgagagactt tgaaggtggt ggcgcgaagg acggagtttg ccggcgtggg gttcgcggtc
     1741 tggtcagaaa aaacgagaac gcttgagcag caatcgagcg tcagttgcgg gacgtttgtc
     1801 cacgcaacgg cctgcacgcc gggaatggtg tgcacggcgt cgatgatgcg tttttgcact
     1861 ggcgggacgg ccgcgccgcg atagccggca gtattgacat caacatccac gagcatcacg
     1921 ttgcgtggat cgacgcccag gccagtgtgc atggagcgaa ggagtccgcg aacggcaaca
     1981 aaggaggagg tgatgagcac agcgcaaagg gcgatctgca ccgtgagcag caagtcgcgc
     2041 gcggtgaggc ggcgtccggg agttgctgtt gaacctgact tgatgacgcc atagggattc
     2101 gtttgcaaca cctggcgcac aggaacagcg ccgaagagga agccggacac aagggcgatg
     2161 agcagcgcga agatatagac atttgcgtcg gggcttacag cggcctggac aggatagcgg
     2221 ggaacgggtc gccacgcgct gagccattgc agaagcaagc tgctggacca gagtcctaca
     2281 gcgcctccca tgaaggagac gagatggctt cggtaaagag ctggcgcagg atgcgggcgc
     2341 gagtggagcc gagggcgaga cgaagggcga tttcgcgcga gcgttcggcg gcgcgagcgg
     2401 agaaggggct gccgagattg gcgcaggcag caagtaagat cagccctgca agcagcatca
     2461 gcgcggcgag aaaggctttt gtggcgagcg cggtattgtc ttcggagcta aatgcgccca
     2521 gatgttcgat ggcgaagacc atcttcttgt catctttggg ataggttttt tccagatagg
     2581 cgccgatgga atttacgtcg gcggtggctt gcgcgggagt gacgccatgt ttcagatgtc
     2641 cgacgatgac ctgaatcgag ccgacgccgc gcgcattgag attgttcacg ccattgacct
     2701 gctcctggtt gatcaaaggc acataaaaac aggcggcgac aaatggaaca gtgccgtgga
     2761 aatcaggcgg tgttacccca atgatggtga acggatgctt gttgagctga acggtgcggc
     2821 cgattacgcc gggatcgtct ttgaaattat tgtgccagta ggagtaggag agcacgaggt
     2881 aaggagcgct gttggggcct cgttcatcgg aagaatggaa gaagcgacca agatagggag
     2941 tgagccgcag ttcatcgaaa tagtttccgc tgactttcca gccccaagtg agcgaaggac
     3001 tttggccgta ttgccctgtg gccagaccga cgccgacgac gccgtaggtc gcgaggccat
     3061 cgaagctgcg gttgcggtcg cggaggtcaa gatagtcggg ataggattga aagcgcgtgg
     3121 tgtcaggcgt gtgctccacg atatagaggc tctgttcgtc agcgacattc aatggacgca
     3181 gaaagagcgc attcaaaatg ctgaaggcca cgacatttgc ggtgatggcc atgccgagag
     3241 tgaggatagc ggcgagagtg aagctcggcg atttgcgcag caggtgcaac gaatagcgga
     3301 tgtcgcgcca gagattgtcc cagaaaagcc agtggtgcga ttcgtagaag cgctcttgcg
     3361 tcttggtgac gttgccgaaa gtgcaatggg cgtgattgtg ggcttcttct tcactcaagc
     3421 cctgttcgcg gaggcgctcc gtttctgcgg caatgtgggc ggcgatttct tcgctgaagt
     3481 cgctggcctt tcgcgtccgg aaaatcctcg ggaacatgct gacctccact aagcctaaac
     3541 ggcgagggcg agtctaaact ctccttctac ttaccttctg cgcttatcca aaccgtgatt
     3601 cttttttttc ggcaacgaaa ttcaatttgc atcctgctgc tcgcgggcgc gggcggggca
     3661 agccccgtcc ctacgattgc gaaaattgtt acctgctatc gttatgcctg tcatcgctag
     3721 gcctgttcgg attccaaaat tcgaccaatt gcgttcgcga agcgtttcca tttcgtggtt
     3781 tccttcgcca gttgggcgcg accggcgcgc gtgagcgaat agaagcgcgc tttgcgattg
     3841 ttcgacgaag tcccccattt cgcggaaatc cagccgcgct cttcgatgcg ctggagcgcg
     3901 ggatagagcg cgccgtgctc gacgagaagc tcttcgtcag aagtttg
//
LOCUS       DATNDT010000527        26632 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_875821, whole genome shotgun sequence.
ACCESSION   DATNDT010000527 DATNDT010000000
VERSION     DATNDT010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 26632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26632
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875821"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2062
                     /locus_tag="VN788_17095"
     CDS             <1..2062
                     /locus_tag="VN788_17095"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HXU22297.1"
                     /translation="ILNAAAEQGAQRAMLAMSHRGRLNVMTHIVGRAAAEIFARFEDV
                     DPRSTLGSGDVKYHIGATGHFHAANGQSIGIHLVSNPSHLEAADPVLIGRTRAKQTRR
                     GENGRQQVVAVTMHGDAAFAGQGICAETLNMSGVPGFTVGGSVSVIVNNLIGFTTVPR
                     DIGSSRFSSDLAKRLPVPIFHVNAEDPDAVVRVGKIALDYRYAFGTPVIVDLIGYRRH
                     GHSEVDDPTITQPLRYRKIEAHPPLWKIYAEKIGADPEPAVKRIREELDAAQKEAREI
                     EKRPPMRQLPRYWNGFVGGRYESSFEVDTAAPEETIAAIGDSLVNHPEGFHVHPKVKK
                     LLEQRVEMAHGQRAIDFGMAEALAFGSLLLQGVPVRLSGQDSRRGTFNQRHAALIDTE
                     TEEEYVPLEHMAPDQGWFEIYNSPLSEAAILGFEYGFSRDFPEALVLWEAQFGDFANG
                     AQIIIDQFIAASEDKWGLLSGVVMLLPHGYEGQGPEHSSARIERFLQLAARDNMQICQ
                     PSNAAQYFHLLRRQALRAWRKPLVVFTPKSMLRNPAASSPLADFSSGCFQTVIPDRGD
                     GSASRVLLCTGKIGHELEAERKRRQQNSPAIVFVEQLYPFPEAELAAEMDRHAAAREF
                     VWVQEEPGNMGAHDFIIPQLEPLARGRAVLSVNRSASASPSTGSAKAHEMEQKTLLTL
                     AFGV"
     gene            2313..3314
                     /locus_tag="VN788_17100"
     CDS             2313..3314
                     /locus_tag="VN788_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HXU22298.1"
                     /translation="MKACLLRASAAVETNPLDFAEVADPRPAAGEVLVRVKMCGVCRT
                     DLHVVEGELPPRKSPVIPGHQIVGIVEKLGEGARRFAVGARVGVAWLHSTDQTCEYCR
                     AGKENLCDHPTFTGYTVDGGYAEYAVAPEQFIYPIPDSFRDDQAAPLLCAGIIGFRCL
                     RLSGIQRGQRLAFYGFGSAAHVAIQVARHWGAEVYACTRGAKHQQLALKLGAVWAGEA
                     ADEPPAKIDAAIVFAPAGELVPPALKGLKKGGVLVLGGIHMSPIPSFDYNLLYQERMI
                     RSVANNTRQDGEDFLRVASEIPVRTQVTVFPLRDANRALNDLKSDRINGAAVLDCAQ"
     gene            3311..3961
                     /locus_tag="VN788_17105"
     CDS             3311..3961
                     /locus_tag="VN788_17105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HXU22299.1"
                     /translation="MKIISLNVGGPRIVHQGDRDVLTGIFKAPVPGPVMLRRLNLDGD
                     RQADLQAHGGRNKAVYAYPSEHYDFWRGQLPEMDLPWAMFGENLTTEGLSEKHAYIGD
                     EFRIGQVARVKVTQPRIPCYKLGIRFGRADIIKRFLASGRSGIYFSVLEEGLVSTGDS
                     IERLHEDERRLSIADVNRAFVRTRENIPLVRRIVSLEILPRGLHDDFAEQLSLLDQ"
     gene            complement(3969..4931)
                     /locus_tag="VN788_17110"
     CDS             complement(3969..4931)
                     /locus_tag="VN788_17110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HXU22300.1"
                     /translation="MKIYFIDVEGGQSTLIVDAQGESLLIDTGWDDFNGRDANRIVDA
                     AKAAGIDHIDWLVITHYHADHAGGVTQLADRMKIKNFADHGPNMENMRVTRASYVAYE
                     KVAAQGNHIVVKPGDTIPFKGMKVQVLTSAGDEIRAPLPGAGQANSLCTSERQPPVDR
                     SENARSVGLLITFGKFRFIDLGDLTTREELGLACPKNLIGTVDLYLVTHHGTAHPGTG
                     DSSNAREIVDALRPRAAVMGNGAIKGGHPVAWKTVHDSPGLEDLWQLHYAVMAGKDHN
                     SAEQFIANLAGPQDEAHYIEALAERDATFTVVNSRNNFQKTYKK"
     gene            complement(5141..5869)
                     /gene="purQ"
                     /locus_tag="VN788_17115"
     CDS             complement(5141..5869)
                     /gene="purQ"
                     /locus_tag="VN788_17115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HXU22301.1"
                     /translation="MKFGVVRFPGSNCDDDAHYAIGSVIGQPVEFIWHQSENVSGFDA
                     IILPGGFAYGDYLRTGAIARFSPVMNAVGRFARAGGLVLGICNGFQILLEAGLLPGAI
                     LRNKRLRFLCRQVHLRVENTDTPFTCAARPGQVLEIPIAHMEGNYFCDAETLAELERN
                     RQIIFRYAHADGRDAGADDFEANPNGSLKAIAGISNRERNVVGLMPHPERAVESALGS
                     TDGLVILRSMVESLSRRAAERVPA"
     gene            complement(5877..6329)
                     /locus_tag="VN788_17120"
     CDS             complement(5877..6329)
                     /locus_tag="VN788_17120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22302.1"
                     /translation="MTATSVDGGILTITGAGSTDAENSMTAATSAAVSVAAKTSTAAV
                     AGTFTVAETSTAEAHSAEADLTVAADSMAEAMVAVGSMVAATAADAGKFPQSSTPQTA
                     GSTLCRPFPFWVRIRCEIESYATNGLAKTHTTRNALVMATVLGMVECG"
     gene            complement(6879..8183)
                     /locus_tag="VN788_17125"
     CDS             complement(6879..8183)
                     /locus_tag="VN788_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HXU22303.1"
                     /translation="MAELATTFEQPFKPVSFPARQVAAVVVGNALEFYDFLTYAFFAT
                     QIGRTFFPSASAENSLLASLATFGAGFITRPIGAVVIGSIGDRVGRKPAMLLSFSMMG
                     IAIIGLALTPSYRVIGLAAPILVICFRLLQGFALGGEVGPTTAFMIEAAPPERRGFYG
                     SLQYATQDVAVLSAGGVGTILASLLSAQQLDDWGWRIAFLIGACIVPFGLYIRRGLDE
                     TLKAPAADEVDAGPAQIRPYLGVAVLGLLMLLAGTVSTYVLNYMTTYASATLHMKATA
                     AFSATLVIGLVEVCFDMTSGWLSDRVGRKPMMITFFLLLVCSVLPGFALISRLRTPAA
                     LLGVSALLAMLLGLGTTPVLITITESIPRQIRSGMVATIYALSISVFGGSTQFTITWL
                     IHATGNPLAPAWYMTGAIAIGLVAMGLIQESAPAKKKVKGAG"
     gene            8370..8651
                     /locus_tag="VN788_17130"
     CDS             8370..8651
                     /locus_tag="VN788_17130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22304.1"
                     /translation="MHIQLNEALALLDGWKNEGTRIRMRASGPAISQELLGTIRAIAG
                     AVVDIGHELKNLELDLAGADFNGDPAHAEYLVCEFSDGRRYAFHVARDA"
     gene            complement(8664..9518)
                     /locus_tag="VN788_17135"
     CDS             complement(8664..9518)
                     /locus_tag="VN788_17135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HXU22305.1"
                     /translation="MACKPEILRSVPLFALLDDDERAVLAGQVELKTFAARERVYKTG
                     DAGGQAYVVISGRVRVTTVDEDHQEVVVDEPASGEFFGFASMLEQTPHQTSAIALDES
                     ACIEVSRDDIEVLLQKKPHAGMDLLTVLGRQFHASQGLIRVRANRNPNEMIEKEATLG
                     ERIADAVARFGGSWAFIIMFLAALVVYTGINITLNKKAWDPYPFILLNLFLSMLAAIQ
                     APVIMMSQNRQDTKDRLRGELDFDVNRRAESEIQGLARKLNLLDEKIDDMGNLLRRTA
                     TNNLATGD"
     gene            10427..11065
                     /locus_tag="VN788_17140"
     CDS             10427..11065
                     /locus_tag="VN788_17140"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU22306.1"
                     /translation="MCQDIRYTRIGYSPEPRRLGTMPWARTDDRRLYSLGSISQGVDT
                     VLNLGAGLDTRPYRLDLPESLIWIEADYPHIVDFKEDRLSKEKPRCQLQRVKLDLSSL
                     PEREPFLASVNARANEMLILTEGVIPYLSLEEAASLADDLKTLDHAAYWIVEYISPQA
                     SKYRGNRRMRQLMQNAPFKFAPKNWFGFSKSTAGAPGKFTICSTKATASIGP"
     gene            11408..11722
                     /locus_tag="VN788_17145"
     CDS             11408..11722
                     /locus_tag="VN788_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22307.1"
                     /translation="MSRPSIETVGVRLTGWAALNRDPLEDRVEYTCPHCGTRGTKAAR
                     RPRSAYHFTDGFVQDLQGEMSTCRACKHSLRLSPVVLVHDRDEQRRFDAVFLADLEVS
                     KN"
     gene            complement(11772..12614)
                     /locus_tag="VN788_17150"
     CDS             complement(11772..12614)
                     /locus_tag="VN788_17150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020592071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HXU22308.1"
                     /translation="MKRFGHLPMSDLAYQDRVFMESTAARPLRILAEYIDPINRLRRA
                     GVGDTIVMFGSARIRPRDRALADLKKVQSAARGRRTPEWSTKIGAAKSIVGMSRYYEE
                     ARELSRRITAWAMTLGAQPKRFVICSGGGPGIMEAANRGAAEAGGSSIGLSIQLPHEQ
                     RPNPYISPDLNFCFHYFFMRKLWFAQMAKALIVFPGGFGTMDELWEMLTLMQTAKLSS
                     RHLILIYGRKYWNRVVNWRHMVRSGTVSEKEFKMLQFTDTVDDAFDHVRGEMEKYHLA
                     LDVE"
     gene            12834..13436
                     /locus_tag="VN788_17155"
     CDS             12834..13436
                     /locus_tag="VN788_17155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22309.1"
                     /translation="MITMQASVIAMFAATMAVVAIVVAAIIVVAAIATLILLRNRTKK
                     LQTRFGPEYNRAVEETGSRYKAEAKLEQLEKRVERYPIRALSLAERDRFQQSWRAVQA
                     KFVDDPIRALAEADQLVGEAMSACGYPVSDFEQRAREISVDHAAVVEHYRAGHDIAVR
                     QSQGKATTEELRQAMIDYRALFDELMGEPERLRTRTAGAS"
     gene            13495..13962
                     /locus_tag="VN788_17160"
     CDS             13495..13962
                     /locus_tag="VN788_17160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22310.1"
                     /translation="MSELNRKDDSLQRESQTPNANAPATDPGSRPLVTPPPSTSPASP
                     QPQSATSRAGTAAGAAPAMAEEKAPLFAANESSNLRSQWDSVQVTFVDDPRNAVQRAD
                     ALVSETIKRLSEVFTSERQKLEEQWGRGADASTEDLRVALRRYRSFFTRLLEI"
     gene            complement(13996..14568)
                     /gene="pyrE"
                     /locus_tag="VN788_17165"
     CDS             complement(13996..14568)
                     /gene="pyrE"
                     /locus_tag="VN788_17165"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015414786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HXU22311.1"
                     /translation="MSQRGRLANLLRAKSLVRGEFTLASGKKSDYYLDCRLTTLDAEG
                     ALLTGYCILEMLDEMGIKPDAIGGLSMGADPLVTAAGVVSAMEKRPLPGFLVRKKAKE
                     HGRQKQIEGMENPRGKKVVIVDDVCTTGGSTQEAIDAAEREGCEVIGVISLVDREEGG
                     SEMLRAKYNYRSIFTARELMKQDDKSAAAQ"
     gene            complement(14565..17231)
                     /gene="gyrA"
                     /locus_tag="VN788_17170"
     CDS             complement(14565..17231)
                     /gene="gyrA"
                     /locus_tag="VN788_17170"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HXU22312.1"
                     /translation="MADELNQQILTPVDIESEMRKSYLDYAMSVIIGRALPDVRDGLK
                     PVHRRILYGMYELGLTSTKAYRKCAKIVGEVLGKYHPHGDSPVYDALVRLAQDFNMRY
                     PLVDGQGNYGSVDGDPPAAMRYTEARLSKIAEEMLADIEKETVEFTPNFDESEQEPLV
                     LPTRIPNLLVNGSSGIAVGMATNIPPHNLREIVEAAILLVENPETTLKQVMKIVPGPD
                     FPTAGYIYGRDGIEQAYKTGRGSFQVRAKAAIEQRGKDRQDIVVTELPYQVNKKSLIQ
                     HIAELINNKKIEGIADIRDESDRDGMRFVIEIKRDEEPEIILNKLFKMTQMQESFGMI
                     LLAIVGGQPRELGLLQLLQLFIEHRVDVVRRRTQYDLRKAQEREHVLLGYRIALDNID
                     EVIKIIRGSSSRANARENLHEFFENKSVRVMVGGKETTLRGVKLDAKKYGITVASSGE
                     GLGLSYIQIDAILELQLHRLTQLSVDEILKELAQIRERVAELQSILASEKKLKGVIVS
                     ELRDVAKSYGDDRRTQIVDRVEEIKLEDLIKDEEMAITVSHAGYVKRTSVETYRHQSR
                     GGKGRIGAKTRDDDFVEHFFVASAHSYILLFTSKGRIYWLKVYEIPEAAAATRGKAIN
                     NLVRFQEGETLKAIVPTRNLEETGRYVFLATKNGTVKKTELADFSNPRPSGIIAISLD
                     ADDELIGAKLTDGKQMIFLASHEGQAILFRESEVRSMGRAAGGVNGMDLAKGDYVVSM
                     DAAQVDFGIVQKEYKKETRDLDELESDEIRESLTSLMLTVAEKGYGKRTPLAEYRVTS
                     RGGKGVINLKSTERNGPVVAALQVREDSDVMIITGHGKVIRVHSGEIREAGRSTQGVR
                     LLRLDEGDRIAAAAAILEEEEAAVANPEPEKK"
     gene            complement(17311..17613)
                     /locus_tag="VN788_17175"
     CDS             complement(17311..17613)
                     /locus_tag="VN788_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22313.1"
                     /translation="MRRGARIRNIIRWILLLLAVAFVAYDLPQTWHNYSKWRLALPSD
                     SVAAGFWQTAFYSDVKEMAIVLGTGIVLWIVLHPRGEHVRASGVETPEGNGGGRHG"
     gene            complement(17615..18847)
                     /gene="glgC"
                     /locus_tag="VN788_17180"
     CDS             complement(17615..18847)
                     /gene="glgC"
                     /locus_tag="VN788_17180"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HXU22314.1"
                     /translation="MPSMRDAIGVLLAGGQGERLWPLTRDRAKPAVPFGGVYRIIDLT
                     LSNCLNSDLRRVFVLTQYKALSLNRHVRRGWSSLMGFGDFIEVLPPQMRVSQHWYQGT
                     ADAVYQNIYSLGSEQSNYVFILSGDHIYKMNYQKMLEQHIAAAADVTVATIEMDAAEA
                     AGKFGVLETDRYWKIVGFEEKPAKPKESPLHPGKINASMGVYLFNTQLLVPILIEDAE
                     NPASSHDFGKDILPRILHKYRVFAYNFRDENKKETSYWRDVGTIDAYHEANMDLVSVS
                     PVLNLYDPGWPLNTWQQQYPPAKFVFADPERMGAALDSIVSGGSIVSGGRVTRSVLGY
                     DVRVNSYCEVEDSIVFNHVNIGRHSRIRRAIIDRHVDLPEGTVIGHDLEADRARYSVS
                     ENGIVVVVRPESMIEEPE"
     gene            19132..19329
                     /gene="rpsU"
                     /locus_tag="VN788_17185"
     CDS             19132..19329
                     /gene="rpsU"
                     /locus_tag="VN788_17185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HXU22315.1"
                     /translation="MAEVVIQEGESLENALRRFKRKVQSEDIIKEIKKHSYYLKPGEK
                     RRAKQALSRKRLRKKQRRELD"
     gene            19468..19782
                     /locus_tag="VN788_17190"
     CDS             19468..19782
                     /locus_tag="VN788_17190"
                     /inference="COORDINATES: protein motif:HMM:NF024843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HXU22316.1"
                     /translation="MEYHDKVLKCAECGAEFVFTAGEQMFFADKGFKNEPKRCKGCKS
                     VRTQGGESAAPPTQRVETKTVCSQCGKETTVPFKPTQGRPVYCRECFQQRRAFGASSA
                     MA"
     gene            19989..21797
                     /locus_tag="VN788_17195"
     CDS             19989..21797
                     /locus_tag="VN788_17195"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU22317.1"
                     /translation="MSAVTKVPTDTSAVPGMRQTARSHHNHVVQFYGEDSALLDALVG
                     FIGQALKAGDAALLIATEAHREELGRRLEAEGIDVSAATAQGCYIPMDAAATLSTFMI
                     DGMPDPILFEEAIGKRVLLARKAAQNENVRLVAFGEMVAILLRNGQPDAALALERLWT
                     KLAKTYSFSLRCAYPMEQFNSQSRRELFMKICDEHSGVIPAGGYAMLSTEDERLRSIA
                     ELQQRAKSLEAQIALHRSEQQLRLLVETVQDYAIFLLDPDGRVATWNLGAQRIKGYQA
                     SEIIGKHFSIFYPEEDIKSGKPQEELRIATAVGRFEDEGGWRLRKDGSRFWANVVITA
                     LRDSSGNLQGFSKITRDITEKMLALEALRKTNRELREEISRRTLAEEKLQESGGSLRK
                     LSGHLLRMQDEERRRLGRELHDSVGQYLAALKMGLDAIHDGSNPQIQECINMADQCMK
                     EVRTISYLLYPPMLEEVGLRSAIPWYIEGFSKRSGIKVDCDIDPKVGRLSRDAELAIF
                     RILQESLTNVHRHSESPTATVRLMLADSTVYLEVSDRGKGIPPSALEPGGDASNTLGV
                     GLRGMIERMHQLGGTMELSSNERGTTVRASLPREKV"
     gene            21920..22396
                     /locus_tag="VN788_17200"
     CDS             21920..22396
                     /locus_tag="VN788_17200"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU22318.1"
                     /translation="MPARILVADDHEVVRQGVRAMIHRLRPKWEICGEAASGDEALQA
                     ILSLKPEVAILDITMPGMSGLDVAYEVAKRHLPTALLIFTMHESERIAGEVREAGAQG
                     FVNKSCAGRDLIAAIEALLGGGTFFGRPIPAGTPEGGGGEPNPRLLFRRQLTLFQT"
     gene            22681..23616
                     /locus_tag="VN788_17205"
     CDS             22681..23616
                     /locus_tag="VN788_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22319.1"
                     /translation="MRLTAKFFSCLLALSLLAIPACAAGIVLSPEAAKTLDEIYNGDT
                     DAAVETAHGIEQAQPDSPVGYLLDAEAQWWKMYCAACEIKWGMLDAWKRGKKPEDASY
                     FALADKAIELARAQIAKSDTAEMHVYVAIGFALKARLYGLRDQKRDIAHAGVAARSEC
                     LRALQLDPDMADATAGLGLYNYYVDTLSGIAKVLRFFMGIPGGDKQEGIRQMKIGMER
                     GAFLAVDSRFYLAKNLRSYDRHYDEALEIAEPLATRYPRNSIFLLLLGNLNQELGRND
                     KASEYSRAAQSAPIADPACANRVRDLANSLLASPH"
     gene            24260..24655
                     /locus_tag="VN788_17210"
     CDS             24260..24655
                     /locus_tag="VN788_17210"
                     /inference="COORDINATES: protein motif:HMM:NF039360.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6152 family protein"
                     /protein_id="HXU22320.1"
                     /translation="MKNTLTALIGAVLMFAVPPAIAHHSRSMFDQSRQITLAGTVKEF
                     QWTNPHCWIQVLVPDPNNPQAAPIEWGVEMGAPVELMRKGWKPRSLNPGDKVTIVVNP
                     LRDGQHGGLVLAVTGPDGKIIGRPAIPVK"
     gene            24680..25498
                     /locus_tag="VN788_17215"
     CDS             24680..25498
                     /locus_tag="VN788_17215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22321.1"
                     /translation="MKRQALAAMVTVLLAGYFCAVHAQTSSKQRLAAFAKLPDWSGIW
                     ELDAFVGQSDGQQFSPEGQRELKAYAAALRPSFTPEYQAKYDAIRKKVADAVAADPAH
                     PPATHQPLCVAPPFPGTSSPGMFEWLVTPEETTFVSTVGAVRHIYTDGRSHPPKDEMW
                     PTLMGDSIGHWEGDTLVVDTIGTRKRLYTGELSGFFFPMSDQLHFTERIRMVNHNEMQ
                     IDYTVEDPIALTKPIHATITHTRVTDFNRMIEETDCEQNDRDPIVNGRFTTVTH"
     gene            complement(25869..26024)
                     /locus_tag="VN788_17220"
     CDS             complement(25869..26024)
                     /locus_tag="VN788_17220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22322.1"
                     /translation="MPTEIWEPISEKKNRRHAPLGMTNSQHVEAHPLVHPRITPSSRK
                     KPRVVGA"
     gene            complement(26068..>26632)
                     /locus_tag="VN788_17225"
     CDS             complement(26068..>26632)
                     /locus_tag="VN788_17225"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006037391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HXU22323.1"
                     /translation="VTSVNRDDQPDGGAEIFARTIAEIRRRVPDCKVEVLIPDFRGNW
                     DALGKVMAARPDVLNHNMETVPRLYRRVRKGALYERSLELLRRAREMAPETPTKTGMM
                     LGLGETREEVLSAMEEIAAQGTHILTLGQYMQPTPEHLPIERYVHPDEFAEFKRLGER
                     MGFKHVEAGPLVRSSYHAFEQEEAARG"
BASE COUNT         5438 a         8436 c         7158 g         5600 t
ORIGIN      
        1 gatcctgaat gccgccgccg agcaaggcgc ccagcgcgcc atgctggcca tgagccatcg
       61 cgggcggctc aacgtgatga cccacatcgt cggccgcgca gcggctgaaa ttttcgctcg
      121 cttcgaggat gtcgatcccc gtagcacatt gggcagcggc gatgtgaagt atcacatcgg
      181 cgccaccggc cattttcacg cagccaacgg gcagtccatc ggcattcatc tcgtttccaa
      241 tccgagccac ctcgaagctg ccgatccagt cctcatcggc cgcacgcgcg ccaaacaaac
      301 gcgccgcggt gaaaacggcc ggcaacaggt agtcgccgtc accatgcacg gagacgccgc
      361 atttgccggc caaggcattt gcgccgagac gctgaacatg tccggcgttc ccggattcac
      421 cgtgggcggc tccgtcagcg tcatcgtgaa taatttgatc ggcttcacca ccgtcccgcg
      481 cgacattggc tcctcgcgct tctcctccga tctcgccaag cgtctgcccg tgccgatttt
      541 tcacgtgaat gctgaagatc cggacgcggt cgttcgcgtg ggcaaaatcg cgctcgatta
      601 tcgctacgcc ttcggcacgc ctgtcatcgt ggacttgatc ggctatcgcc gacacggcca
      661 tagcgaagtg gacgacccca ccatcacaca gccattgcgc tatcgcaaaa tcgaggcgca
      721 tccgccgctg tggaaaattt atgccgagaa aattggcgcc gatcccgagc cggccgtcaa
      781 acgcatccgc gaagaactag acgccgccca gaaggaagcg cgcgaaatcg aaaagcgccc
      841 gccgatgcgc caactcccac gctattggaa tggattcgtc ggcggccgct acgaatcctc
      901 attcgaagtg gataccgccg cccccgagga aaccatcgcg gccatcggcg attcgctcgt
      961 caatcatccc gaaggatttc atgtccatcc aaaagtaaaa aagctgctcg aacagcgcgt
     1021 cgaaatggcc cacggccagc gcgccatcga ttttggaatg gccgaagcgc tggccttcgg
     1081 ctcgctactt ttgcaaggtg tgcctgtgcg tctctcgggg caggatagcc gccgcggcac
     1141 tttcaaccag cgccacgccg ctctcattga caccgaaact gaggaagaat acgtcccgct
     1201 cgaacacatg gcgcccgatc aaggctggtt cgaaatttat aattcgccgc tttccgaggc
     1261 cgccatcctc ggcttcgagt acggcttcag ccgcgatttc cccgaagctc tggtgctatg
     1321 ggaagcgcag ttcggcgact tcgccaacgg tgcgcagatc atcatcgatc agttcatcgc
     1381 cgcgagcgaa gacaaatggg gcctgttgtc cggagtcgtg atgcttctcc cgcacggata
     1441 cgaaggccag ggccccgaac attccagcgc gcgcatcgaa agatttctcc agctcgccgc
     1501 gcgcgacaac atgcagattt gccagccgtc gaacgccgcg caatattttc atctgttgcg
     1561 tcgccaggcg ctgcgtgcgt ggcgcaagcc cctcgtcgtt tttactccaa agagcatgct
     1621 gcgcaatccc gcggcatcct cgcccttggc agatttctct tcgggatgtt tccaaactgt
     1681 gatccccgat cgcggcgatg ggtcagcctc gcgcgtcctt ctctgcaccg gaaaaattgg
     1741 ccacgaactc gaagccgagc gaaagcgtcg tcagcaaaat tcgccggcga ttgttttcgt
     1801 cgagcagctt tatccttttc ccgaagccga acttgccgcc gaaatggatc gccacgcggc
     1861 cgcccgcgaa ttcgtctggg tccaggaaga acccggcaac atgggcgcgc acgatttcat
     1921 cattccgcag ctcgagcctc tggcacgtgg ccgcgccgtc ctatcggtaa atcgctcggc
     1981 cagtgccagc ccctccaccg gctccgcaaa agcccacgaa atggaacaga aaaccctgct
     2041 aactctagcc ttcggcgtct agttgtattt cgtaggggca cagcatgctt tgcccggcaa
     2101 ggatacttgg ctacctgttt gctccacccc gcactacggg cggctcgtgt ggagtgctgc
     2161 agccattcgc cagcgttcta cttgcctttg ccgccatagg atcgcgcgtc attgggggcg
     2221 aagtgtcccg cgcctttgcc tttgcgcgaa gcgccggcac acgcagtcga agaatctctc
     2281 ttcgatgtaa actgagacct gcggtcaaca acatgaaagc ttgtcttttg cgcgcgtccg
     2341 cagcagtcga gaccaatccc ctcgacttcg ccgaagttgc cgacccgcgc cccgcggccg
     2401 gcgaagtcct cgtgcgcgtg aaaatgtgcg gagtctgtcg caccgatttg cacgtagtcg
     2461 aaggcgaact cccgccccgc aaatccccgg tgattccagg acatcaaatc gtcggcatcg
     2521 tcgaaaaact cggcgaaggt gcgcgccgct ttgccgtcgg cgctcgcgtc ggagtcgcct
     2581 ggctgcatag caccgatcaa acctgcgaat attgccgcgc cgggaaagaa aatctctgcg
     2641 accatccgac tttcaccggc tacaccgtgg atggcggcta cgccgaatac gccgtcgccc
     2701 ctgagcagtt catctatccg attcccgatt cttttcgcga cgatcaagcc gcgccgttgc
     2761 tctgcgccgg aattatcggg tttcgctgtc tgcgcctttc gggcatccag cgcggccagc
     2821 gtctcgcctt ctacggtttt ggctcggccg cgcacgtcgc aattcaagtc gcgcgccact
     2881 ggggagcgga agtgtacgcc tgcacccgcg gcgcgaagca ccagcagctt gcgctcaaac
     2941 tcggcgccgt atgggctggc gaagccgcgg atgaaccgcc cgccaaaatc gacgccgcaa
     3001 tagttttcgc ccccgccgga gagctggttc cccccgcgct caaaggcctg aaaaagggcg
     3061 gtgtgctcgt cctcggcggc atccatatga gtccaattcc ctctttcgat tacaatttgc
     3121 tttaccagga gcgcatgatc cgcagcgtgg ccaataatac gcgccaggat ggcgaggatt
     3181 ttctccgcgt cgcttctgaa attcctgtgc gcactcaggt cactgttttc ccgctgcgcg
     3241 acgcgaatcg cgctctcaac gatctcaaaa gcgaccgaat caatggcgcc gccgttctgg
     3301 attgcgcgca atgaaaatta tttcgctcaa tgttggcggg ccccgcatcg tccaccaggg
     3361 tgaccgcgac gtactcaccg ggatcttcaa agcgccggtg cccggcccgg tcatgcttcg
     3421 ccgccttaat ctcgatggcg atcgccaagc cgacctgcaa gctcacggcg ggcgcaacaa
     3481 ggctgtctac gcctaccctt ccgagcacta cgatttctgg cgcggccagc ttccggagat
     3541 ggatttgccc tgggccatgt ttggcgagaa tctcaccacc gaaggactaa gcgagaagca
     3601 tgcctacatc ggagacgaat ttcgcatcgg tcaggtagcg agggtcaagg tcacgcagcc
     3661 gcgcatcccc tgctacaaac tcggaatccg cttcggacgc gccgacatca tcaaaagatt
     3721 tctcgccagc ggccgctccg gaatttactt ttccgtttta gaagaaggtc tcgtcagcac
     3781 aggagactcc atcgagcgcc tgcacgaaga cgaacgccgc ctcagcatag cggacgtcaa
     3841 tcgcgccttc gttcgcacgc gcgaaaatat tccgctggtg cgccgcatcg taagcctgga
     3901 aattcttccg cgcggccttc acgacgattt cgccgagcag ttgtctctgc tcgatcaata
     3961 atccggcgct acttcttata agttttctgg aaattgtttc gggaatttac caccgtgaaa
     4021 gttgcgtcgc gctctgccaa tgcttcgatg taatgtgcct cgtcctgcgg acccgccaga
     4081 ttggcgatga attgttcggc cgagttgtga tccttccccg ccatcaccgc atagtgcaat
     4141 tgccacaaat cttccaaccc gggtgaatcg tgaacggtct tccacgccac gggatgcccg
     4201 cctttaatcg ctccgttgcc catcaccgcc gcgcgaggac gcagtgcatc cacaatctcg
     4261 cgggcgttgg acgaatctcc agttcccggg tgcgcggtgc cgtggtgcgt caccagataa
     4321 aggtccacgg ttccgatcaa atttttcgga caggccagac ccaattcctc ccgcgtggtc
     4381 aaatcgccca gatcgatgaa tcgaaatttg ccaaacgtaa tcagcagccc cactgacctg
     4441 gcattttcac tccggtccac cggcggctgc cgttcagatg tgcacagcga attcgcctgc
     4501 cccgctcccg gcagcggcgc gcgaatttcg tctcccgccg aagtcagcac ttggactttc
     4561 attcccttga acggaattgt atcgcccggc ttcaccacga tgtggtttcc ctgcgcagct
     4621 actttttcat acgccacgta actcgcccgc gttacgcgca tgttttccat gttcgggcca
     4681 tggtcggcga aattcttgat cttcatccgg tccgcaagtt gcgtcacgcc tccagcgtgg
     4741 tccgcatgat agtgcgtgat caccagccaa tcgatgtggt cgattcccgc ggccttcgcg
     4801 gcgtccacga tgcgattcgc gtcccgcccg ttaaaatcat cccaaccggt atcgatcagc
     4861 agcgattcac cttgcgcatc cacaatcaaa gttgactgcc cgccctccac atcgatgaaa
     4921 tatattttca attgcttcgc cgcgcgtgtc gctgcgctcg cggtcaggca agcgcacacg
     4981 agccataccg ccgcgataag ccctcgtcgc atcattctcc cgtttttaaa aaaaatagcg
     5041 gacctgattc cgcacgaagc tgcacagcag tcttaattgt agcgaagtat tgttacagac
     5101 ccataaccac cgcttggtgg ttgtctactg gattagccaa ttaggcggga acgcgctcgg
     5161 cagcgcgtcg cgaaagactt tccaccatcg accgcagaat caccagccca tccgtcgaac
     5221 ccagtgcgga ttccacggcg cgctcgggat gcggcatcaa gcccaccaca ttgcgttcgc
     5281 gattcgagat gccggcgatc gcctttagcg aaccattggg attcgcctca aaatcgtcgg
     5341 cgcctgcatc acggccgtcc gcgtgcgcgt aacgaaaaat aatctgcctg ttgcgttcga
     5401 gttcggcgag cgtttccgca tcgcaaaaat aattcccttc catgtgcgcg atcggaattt
     5461 ccaacacttg ccccggccgc gccgcgcacg tgaacggcgt gtccgtgttt tccactcgca
     5521 gatgcacctg ccggcacaaa aaccgcagcc ttttgttgcg caggatggcg cccggcagca
     5581 accccgcttc gagcaaaatt tgaaatccat tgcaaatccc gagtaccaat ccccccgcgc
     5641 gcgcgaaccg tcccaccgcg ttcatcactg gagaaaaacg cgcgatcgcg ccggtgcgca
     5701 agtagtcgcc gtaggcaaaa ccgcccggaa gaataatcgc gtcgaagccg gaaacatttt
     5761 ccgattggtg ccaaatgaat tcgactggct gcccaatcac cgacccgata gcgtaatgcg
     5821 catcgtcgtc gcaattcgat cccggaaatc gcaccacgcc gaatttcatg tgcgcctcat
     5881 ccgcattcta ccatgcccag tacggtagcc atgacgagcg catttctcgt cgtgtgggtt
     5941 ttcgccagtc cgttcgtagc atagctctca atctcgcacc ggattcgaac ccaaaaagga
     6001 aacggccggc agagcgtgct gccggccgtt tgaggtgtac tgctttgggg gaatttacct
     6061 gcgtccgccg ccgtggccgc caccatggaa cccaccgcca ccatggcctc cgccatggaa
     6121 tccgccgcca ccgtgagatc cgcctccgcg gaatgagctt ccgccgtgga agtttccgcc
     6181 accgtgaaag ttcccgccac ggctgccgtg gaagttttcg ccgccacgga aaccgccgcg
     6241 gaagttgccg cggtcatgga attctctgcg tccgtcgagc cggcgccagt gatcgtcaaa
     6301 atcccgccgt ccacggaagt cgcggtcatg gaatggtcgc ccgtaaaagc ggccgcggaa
     6361 ccacggccct gcgccgatga agatgccgct ggagaaccac tcaggcccgt agtagccata
     6421 cggcgcgcac gcgtatgggt agtatccata atagccgtac gtgcacaccg gcggaccgta
     6481 cacggactcg ccgtacaccg ggccgcctat tccgatgccc accgaaaatc tctgtgcatg
     6541 tgcgaagcct gctgtgaaca ggccgacgct cagtaccgcc aaaattcgta catatttcat
     6601 tttggcctcc tcttgaaacc gctccaacct gtcctacagc cgcttcgggc tacccgggtt
     6661 gagtcacctt ccggcccgta cagtgcatag ggcttgccaa aatgagctac tgtctaataa
     6721 aaaaataagc ctcctattat cagatggata gatatggaca agccccttgt cgcccgcgcg
     6781 cggccgctaa gggtcggtgg tgctttttaa gccggactgg tggtcctcaa ccagggggtg
     6841 tttcaggtag cttgcttggt gccacgcgtc atgccgactc agccagcacc cttaaccttc
     6901 ttcttcgccg gcgcggattc ctgtatcagt cccatcgcca caagtcctat cgcaatcgcc
     6961 cccgtcatgt accacgctgg cgccaacggg ttgcccgtcg cgtgaatcag ccaggtgatg
     7021 gtgaactgcg tggatccccc gaagacggaa atcgacagcg catatatcgt tgccaccatc
     7081 ccggaacgga tttgccgcgg tatcgactcc gtaattgtga tcagaaccgg agtcgtgccc
     7141 agccccagca gcatcgcgag caacgcgctg acccccaaca gcgctgcggg tgtgcgcagg
     7201 cgcgagatca gtgcaaagcc cggcagcacg ctgcacacca gcagcaggaa gaacgtgatc
     7261 atcatcggtt tgcggcccac cctgtccgaa agccagccgc tagtcatgtc gaagcacact
     7321 tccactaagc cgatcaccag tgttgcgctg aaggccgcgg tcgctttcat gtgcagcgtg
     7381 gcggaggcat aagtggtcat gtaatttagg acgtaagtag ataccgttcc cgcgagcagc
     7441 atcagcagcc ccagcacggc cacacccaag tagggacgaa tctgcgcggg ccccgcgtcc
     7501 acttcgtcgg ccgccggcgc cttcaacgtt tcatccagcc cccgccggat gtacagccca
     7561 aaggggacga tgcatgctcc gatcaggaac gcgatgcgcc atccccaatc gtcgagttgt
     7621 tgcgcgctca gcaaactggc caggatcgta cccaccccgc ccgcgcttag caccgcaaca
     7681 tcctgcgtgg catattgcag cgaaccataa aacccgcggc gctccggcgg agccgcctcg
     7741 atcatgaaag ccgttgttgg tcccacttcg ccgcccagcg cgaaaccctg cagcaggcgg
     7801 aagcagatca ccagaatggg cgcggccaag ccaatcacgc gatacgacgg cgtcagcgcc
     7861 agcccgatga tcgcaatccc catcatcgag aaagaaagca gcatcgccgg cttccgtccc
     7921 actcggtcgc caatcgagcc gatcaccact gcgccgatgg gccgcgtaat aaatccggcg
     7981 ccaaacgttg caagcgacgc cagcaggctg ttttcggcgc ttgcggaggg aaagaaagtg
     8041 cggccaatct gagtggcgaa aaaggcgtag gtcagaaagt cgtagaattc aagcgcattc
     8101 cccaccacca cggccgccac ctgccgcgcg ggaaacgaca ctggtttgaa cggctgctcg
     8161 aaagttgtcg ccaactcagc cattcccgtt cagcaccccg ctgggagtct aggcaaagtc
     8221 cggcccaaac tctctcctgt ccacgtggcc tgtgcgcatc gaacttgaat gtcggcggat
     8281 catagcacag caaccctcga cccgttggcc ggctactgtt tgaggaccta ccgaaaccga
     8341 tctgtctggc gggctagaat gcctcattca tgcacataca gctaaacgaa gcactcgcgc
     8401 tgctggatgg ttggaaaaat gaaggaacgc gcatccgaat gcgcgcttcc gggccagcaa
     8461 tctcacaaga actgctcggc accattcgag ccatagccgg agcggtcgtg gacatcggac
     8521 atgaactaaa gaatttggag cttgatttgg cgggggcaga cttcaacggc gacccggccc
     8581 atgctgaata tctcgtctgc gaattctccg acgggcgccg ttacgctttt cacgtcgcac
     8641 gcgatgcgta attgagctgg gccttagtct ccggtcgcca ggttattggt cgctgttcga
     8701 cgcagcaaat tccccatgtc gtcaatcttc tcatcgagca aattcaactt ccgcgccaag
     8761 ccttgaatct cggattctgc ccgccgattt acgtcaaaat cgagctcacc gcgcagccga
     8821 tcctttgtgt cctgccgatt ctggctcatc atgatgaccg gcgcctgtat cgcggctagc
     8881 atggaaagaa acaggttgag cagaataaac ggatacggat cccaggcttt tttgttaagc
     8941 gtaatgttga tccccgtgta tacgaccagg gcggccagga acatgataat gaacgcccac
     9001 gatccgccaa accgtgccac cgcgtccgcg attcgctcgc ccagcgttgc ctccttctcg
     9061 atcatctcgt tcggattgcg attcgcgcgg actcgtatca gcccttgcga ggcatggaac
     9121 tggcggccga gcaccgtcag cagatccatc ccagcgtgcg gcttcttctg cagcagcacc
     9181 tcaatatcgt cgcgtgagac ctcgatgcac gcgctttcgt caagagcgat cgcgctcgtc
     9241 tggtgcggcg tctgctccag catcgacgcg aatccgaaga actcgccgct cgcaggctcg
     9301 tcgaccacca cttcctgatg gtcctcatcc acggtggtca ctcggactct gccggagatc
     9361 accacatacg cctggccgcc cgcgtctccg gtcttataga cgcgttcgcg cgccgcaaat
     9421 gtcttgagct ccacttgacc ggccagcacc gccctttcat cgtcgtccag caacgcaaag
     9481 agtggaacgc tcctcaaaat ttccggcttg cacgccatag tcgtctcctt gaattacggg
     9541 ttgatccgct ttgggcacgt cgcgtgcccg gacaacgcta cctcattatc caacgagtag
     9601 catgctcctc tcgaagcgag cacgtttggg gggccattcc tcattctgca tgtcctcacg
     9661 acgaaaggga gccaagtttc gcgcgtcttg cctgtcaccg cagtgcacgc gcggcatcgc
     9721 cgtcattccg agcgaagccc gcgctttttt cttcgcgcgg tatttgcgcg gcgcggggcg
     9781 cagttgagga atctctcttc gatgtaacac ttgcctcgcc tcgagtcgag aagctcttgg
     9841 tatttcggtg tcgcgctcgg agagtgggcg taagcaaaaa aaagagggcg actcggtttc
     9901 acccgtgtcg ccctcgttcg attgagtcgc gaagtttccg cctcccgact cgacttagtc
     9961 ggaggcggtt cggttttacc cagaccgcct cttgtaaatc gagtcgcgaa gccctaactt
    10021 gccggttcac tcacgatcac gtctcggttt taccctcgcc gcatccttga gtgcatcgca
    10081 aagcttttcg cttctgcttg cgtccagcgg ccactcggtt tcatccgagt cgccgtgaat
    10141 tcagcgaagc ttctcctcga tccaactcat tggaagctgg ctcggtgtta cctcgccgtt
    10201 cctttgcatc gaaccttgga gcgtccttcc cgatcaatct gcgcgcatag taaaagcagc
    10261 gatcaatgct agcaagagtt atttcccgaa acgcctgtgg aaacctgtgc aagcctgtgg
    10321 acgctgtgca aaaaaaaggt cgtatgccgc ccatttcacc cgcagcgccg ccgtatcttt
    10381 cgtaggggcg tagcgtgctg tatggtttca tgacttaggt tacaggttgt gtcaggacat
    10441 ccgttacacc cgaatcgggt atagtccgga gccgaggagg ctcgggacca tgccatgggc
    10501 gaggacggat gatcgacgac tatattcgtt gggctccatt tcccagggcg tggacacagt
    10561 tctgaatctc ggcgccggcc tcgatacgcg cccctaccgc ctcgatctgc ccgaatctct
    10621 gatctggatc gaagcagact atcctcatat cgtcgacttc aaggaagatc ggctgtcgaa
    10681 ggaaaagccg cgctgccagc tccagcgcgt gaaactcgat ctctccagtc tgcccgaacg
    10741 cgaaccattc cttgcgagcg tcaatgcgcg agcaaatgaa atgctgattc taacggaagg
    10801 agtgattccc tatttgagtc tcgaggaagc cgcttctctt gccgacgatc tgaagacgct
    10861 cgatcacgcc gcttactgga tcgtcgaata catttcgccg caggcttcta aataccgggg
    10921 aaatcgtcgc atgaggcaac tgatgcaaaa tgctcccttc aaattcgcgc cgaagaactg
    10981 gttcggtttt tcgaagagca cggctggcgc tccagggaag ttcactattt gctcgacgaa
    11041 ggcgaccgcc tccatcggcc catgaagctg cctccatttc taatgctcct tttcgtcatt
    11101 cggggaatct tcgcctcaaa ggagcagcgc gccgccttcc gcaagtttca aggttacgcc
    11161 accctcgaac ccacgaattc gtagcggcga ccgtggtcga agaccgcgac tgcgctgcca
    11221 tttgcgctcc cgatatgagc agacaccggc cgccgaggcc ggttccagct tgttcatagg
    11281 gcgctttggg tccgtaagcc actatttagc agcaacttgc agtttttaat cggatcgtgc
    11341 atcgcgtccc ccatttcgcg cgtctaaaag ccgtgtgaac ttcagaatgg gggtccagat
    11401 agctatcatg agccgtcctt ccatagaaac agttggcgtc cgattgacgg gctgggccgc
    11461 gttgaatcgc gatcccctgg aagaccgcgt tgaatacacg tgtcctcatt gcgggactcg
    11521 cggcaccaag gccgcacgtc gtccccgctc tgcgtaccat tttaccgatg ggttcgtgca
    11581 ggaccttcag ggcgagatga gcacctgccg cgcgtgcaag cactcgctcc ggctttcgcc
    11641 agtagttctg gtgcatgacc gagacgagca gcgtcgcttc gacgccgtct tcctggccga
    11701 cttagaagtt tcgaagaact aatcggtcgc tgggggattt cgacaaccgc gcgcaacggt
    11761 gtgggttcgc ctcactccac gtcgagcgcg agatggtatt tctccatttc tccgcgcacg
    11821 tggtcgaacg cgtcatccac cgtgtccgta aactgcagca tcttgaattc cttctcactt
    11881 accgtaccgc tgcggaccat gtgccgccaa ttcaccaccc tgttccagta tttccgccca
    11941 taaatcagga tcaagtgccg cgaagaaagc tttgccgtct gcatcagcgt aagcatttcc
    12001 caaagctcgt ccatggtgcc gaagccgccc ggaaaaacga tcagcgcctt ggccatttgc
    12061 gcgaaccaca acttgcgcat gaaaaagtag tgaaagcaga aattcagatc cggactgata
    12121 tagggattcg gccgctgctc gtgcggaagc tgaatgctca accctatgga cgatccgccc
    12181 gcttctgccg cgccccgatt cgccgcctcc atgattcccg ggccgccgcc cgagcagatc
    12241 acaaaccgct tcggctgcgc tcccaacgtc atcgcccacg ccgtgattcg gcgtgaaagc
    12301 tcgcgggcct cttcataata gcgggacatt cccacgatgg atttcgccgc cccgattttg
    12361 gtgctccact ccggggtgcg gcggccacgg gcggcggact gcactttttt taaatcggcc
    12421 agtgcacggt cgcgcggccg aatgcgcgcc gacccgaaca tcacaatcgt atctcccacc
    12481 ccggcgcggc gcagccggtt gatcggatca atatactcgg ccagaatgcg caacggcctg
    12541 gcggcagtgc tctccataaa aacccgatct tgataggcca gatcggacat aggcagatgg
    12601 ccgaaacgct tcatcagtcc tcctcgcaaa aattgcaaag aacagtttag cctgtccgca
    12661 cggcgagcat tggggagaga tgagcccaac tgccgatgtt gtaacctcat ccggacccaa
    12721 ccttttcgcg gctctctttc ctgatacaga cttattccct cgccagtaca gccaatcttg
    12781 gatttcaaac ccgtctcatc cggattcata tttgcgccgg agcgtctact ctcatgatca
    12841 caatgcaagc atctgtgatt gcaatgttcg cggccacgat ggcggtagtt gcgatcgtgg
    12901 ttgcagcgat catcgttgtc gctgccatcg ccaccttgat attgctgcgc aaccggacca
    12961 agaagctgca aactcggttt ggcccggaat acaatcgcgc ggtcgaagag acggggagtc
    13021 gatacaaagc cgaggcgaag ttggaacaac tcgagaaacg cgtcgaacgg tacccaattc
    13081 gcgcgctctc tcttgcggaa cgcgaccgat tccagcagtc ctggcgggcc gttcaagcca
    13141 agtttgttga tgatccgatt cgcgctttgg cggaagcgga tcaacttgta ggcgaggcca
    13201 tgtccgcctg cggttatccc gttagcgact ttgaacagcg cgcccgggaa atctcagtcg
    13261 accatgctgc tgtcgtcgaa cattatcgcg ccgggcacga tatcgctgtt cgccagtcgc
    13321 agggcaaagc caccaccgag gaactgcggc aggccatgat cgattaccgc gcgctgtttg
    13381 acgaactgat gggagaaccg gagcgtctgc gcacacggac ggccggcgca agttgagcac
    13441 acgagaaggg tgtacgccat tttgcaagat gaatcgagaa acgaggaacc atccatgagt
    13501 gaactgaatc gaaaagatga ttcgttgcag cgagaatctc agactcccaa cgcgaatgcg
    13561 ccggcgaccg atccgggctc ccgcccgcta gtcacgccgc cgccttcgac ttcacccgcg
    13621 tccccgcagc cgcagtccgc cacgagccgc gcgggaacgg ctgccggtgc ggcgccggcc
    13681 atggcggaag aaaaagctcc tctattcgcc gcgaatgaat caagcaatct gcgctcccaa
    13741 tgggatagcg tccaggtaac cttcgtggac gatccccgca acgccgtgca aagagcggac
    13801 gcgctcgttt cagaaaccat caaacggttg agtgaagtct tcactagcga gcggcaaaaa
    13861 cttgaagagc agtggggccg tggcgcggac gcttccaccg aggatcttcg tgttgccctg
    13921 cggcgctacc gttcattctt cacgcggctt ctagaaatct gatcgcgcgt ccagcgttca
    13981 agggggcgcc gaaacttatt gcgccgccgc ggacttatca tcctgtttca tcaattcgcg
    14041 cgcggtgaaa atcgagcggt agttgtattt cgcgcgcagc atttcggagc cgccctcttc
    14101 gcggtccacc aggctgatca cgccgatcac ttcgcaacct tcacgctccg ccgcgtcgat
    14161 ggcttcctgt gtcgagccgc ccgtggtgca tacgtcgtca acaatcacca ctttcttccc
    14221 gcgcggattc tccatgccct caatttgttt ctgccgtcca tgttctttcg ctttcttgcg
    14281 aaccagaaat ccgggcagcg gccgcttttc catcgcgctc accactcctg ctgccgtaac
    14341 cagcggatcg gcgcccatgc taagaccgcc aatcgcgtca ggcttgattc ccatttcatc
    14401 gagcatctcg agtatgcaat atcccgtaag cagcgcccct tccgcatcca gcgtggtcag
    14461 cctgcaatcg aggtaataat cgctcttctt gccggaagcg agcgtgaatt cgccgcgcac
    14521 cagcgattta gcgcgcaaca gatttgccag ccgccctcgc tgactcactt cttctccggt
    14581 tcaggattcg cgacggcggc ctcttcttct tccagaatag cggcggctgc ggcaatgcgg
    14641 tcaccttcat cgagccgcag caatcgcact ccttgcgtcg accgacccgc ttcgcgaatt
    14701 tcgccggaat gcacgcgaat cactttgccg tgtccggtaa taatcatcac gtcggaatcc
    14761 tcgcgcacct gaagggccgc gaccactggc ccgttgcgct ccgtcgactt caggttgatc
    14821 acgcccttgc cgccgcgcga tgtgacgcga tattccgcca gcggcgtgcg cttcccgtag
    14881 cccttttccg ccacggtcaa catcaacgac gtgagcgatt cgcgaatctc gtcgctttcc
    14941 agttcatcaa gatcgcgcgt ctctttcttg tattccttct gcacgattcc gaagtccacc
    15001 tgcgcggcgt ccatcgagac tacgtaatcg cctttcgcaa ggtccatccc attcacgccg
    15061 cctgcggcgc ggcccatcga acgaacttcg ctttcgcgga aaagaatcgc ctgcccctcg
    15121 tggctcgcga ggaaaatcat ctgctttccg tcggtgagct tcgcaccaat cagttcatca
    15181 tcggcgtcaa ggctgatggc gatgattccc gacggccgtg gattcgaaaa atcggcgagt
    15241 tccgtctttt ttaccgtgcc attcttagtg gccaggaaaa cgtagcgccc cgtttcctcc
    15301 aggttgcgcg tcggcacaat ggcctttagg gtttcgcctt cctggaagcg gaccagattg
    15361 ttgatcgcct tgccgcgtgt ggccgcggcc gcttcgggaa tttcgtaaac tttcagccaa
    15421 tagatccgcc ccttcgacgt gaatagcaga atataactgt gcgccgaggc cacgaaaaag
    15481 tgttcgacga aatcgtcgtc gcgtgttttc gcaccgatgc gccccttgcc gccgcgcgat
    15541 tgatgccggt acgtttcaac cgacgtgcgc ttcacgtagc ccgcgtggct caccgtgatc
    15601 gccatctcct cgtccttgat caggtcttcg agcttaatct cttccacgcg atccacgatt
    15661 tgcgtgcgcc ggtcgtctcc atacgacttc gccacgtcgc gcagctcgct gacgatcacg
    15721 cctttcagct tcttctcgct tgccaggatg gactgcagtt cggccacgcg ctcgcggatt
    15781 tgcgccagtt ccttcagaat ttcatccacg gaaagctgcg tcaggcgatg caattgcaat
    15841 tcgagaatgg cgtcaatctg aatgtagctg agccccagtc cctcgccgga gctggccacg
    15901 gtgatcccat attttttggc gtcgagcttc acgccgcgaa gcgtggtttc cttgccgccc
    15961 accatcacgc gcacggactt gttctcgaag aattcgtgca aattctcgcg ggcgttcgcg
    16021 cggctggacg atccgcgaat gattttgatc acctcgtcga tattgtccag tgcgatgcgg
    16081 tacccgagca ggacgtgctc gcgttcctgc gccttgcgca agtcatattg cgtgcgccgc
    16141 cggaccacat ccacgcggtg ctcgatgaaa agttgcagca gttgcagcag cccaagctcg
    16201 cgcggctgcc cgccaacgat ggccagcaga atcatgccga acgattcctg catctgcgtc
    16261 atcttgaaaa gcttgttcaa gatgatttcc ggctcttcgt cgcgttttat ttcgatcacg
    16321 aagcgcatgc catcgcgatc gctctcatcg cgaatgtccg cgatcccctc aattttcttg
    16381 ttattgatca gttcggcgat gtgctggatc aggctctttt tgtttacctg gtagggcaat
    16441 tcggtgacaa caatgtcctg gcgatccttg ccgcgctgct caatggcggc cttcgcgcgc
    16501 acctggaacg agccgcggcc cgtcttgtac gcttgctcga ttccatcgcg gccgtaaatg
    16561 tagcccgccg tcggaaaatc gggtccgggg acaatcttca tcacctgctt cagagtcgtt
    16621 tccggattct ccacgagaag gatcgccgcc tctacaattt cgcgaagatt gtgcggcgga
    16681 atattcgtcg ccatgcccac cgcgattccg gacgagccat tcaccagtaa attcggtatg
    16741 cgtgtgggaa ggacgagtgg ttcctgctcg ctttcatcga aattcggtgt gaactcgacg
    16801 gtttcctttt caatatccgc gagcatttcc tcggcgattt tcgaaagccg cgcctcggtg
    16861 taccgcatcg ccgcgggcgg atcgccgtcc accgacccat agttgccctg gccgtctacc
    16921 agcggatacc gcatgttgaa atcctgcgcc aggcgaacca gcgcgtcata caccggcgag
    16981 tccccgtgtg ggtgatattt gccgagcact tcgccaacga tcttcgcgca cttgcgatac
    17041 gccttcgtcg acgtgagccc tagctcgtac atgccgtaga gaatccgccg gtggaccggt
    17101 ttcagtccgt cgcgcacatc cggcagcgcg cggccgatga ttacgctcat cgcgtaatcg
    17161 agatacgact tcctcatctc cgattcgata tccaccggag tcaaaatctg ctggtttagc
    17221 tcgtccgcca ttcgtccctc aaagtaattt ttgttccgcc acaatttgcg ggatgcttac
    17281 cggttagacc aggttcgtca gtgcacggct tcagccgtgc cgtccgccgc cattgccctc
    17341 aggggtttca acccccgagg ccctcacatg ctcgccacgc ggatgcaata cgatccagag
    17401 cacaatgccc gtccccaaaa cgatggccat ctccttcacg tccgaataga aagcggtttg
    17461 ccagaatcca gcagccacgg aatcggaggg gagcgcgagg cgccacttac tgtaattatg
    17521 ccacgtttgc ggcaagtcgt atgcgacaaa cgccaccgca agcagcaata aaatccagcg
    17581 aattatgttc cgaatgcgcg caccgcggcg catatcattc cggttcctcg atcatggatt
    17641 cggggcggac caccacaacg atgccgttct cggaaacgga atagcgtgcg cggtccgctt
    17701 cgagatcgtg cccgatcacg gtgccttcgg gcaaatctac gtgccggtcg atgatcgcgc
    17761 gccgaatgcg gctgtgcctt ccgatattca cgtggttaaa cacgatcgag tcttccacct
    17821 cgcaatagct gttcacgcgc acatcgtagc cgagcaccga tcgcgtaacg cgcccccccg
    17881 atacgatcga cccgcccgac acgatcgaat ccagcgccgc gcccatgcgc tccggatcgg
    17941 cgaacacgaa tttcgccgga ggatattgct gctgccaggt attcaggggc cagcccggat
    18001 cgtacaggtt cagcaccgga ctcaccgaca ccagatccat gttagcttcg tggtacgcat
    18061 caatcgttcc cacgtcgcgc caataggaag tttctttttt attttcatcc cggaagttgt
    18121 aggcaaaaac gcgatattta tgcaggatgc gcggcaaaat atctttcccg aagtcgtgcg
    18181 acgacgccgg attctcggcg tcctcaatca gaatgggcac caacagctgc gtgttgaaga
    18241 gatacacgcc catcgacgcg ttgattttcc ccggatgcag cggcgactcc ttgggcttgg
    18301 ccggtttttc ctcgaatccc acgattttcc aataccggtc ggtttccagc acgccgaatt
    18361 tcccggcagc ttcggcggcg tccatttcga tcgtggccac ggtcacgtcc gctgcggctg
    18421 ctatatgctg ctccagcatc ttctgataat tcattttgta gatgtggtcg ccggagagaa
    18481 tgaaaacgta attcgattgc tcgctgccga gcgaataaat attctgatac accgcgtccg
    18541 ccgtgccctg ataccagtgc tgcgagacgc gcatctgcgg cggcagcacc tcgatgaaat
    18601 cgccgaagcc catcagcgat gaccacccgc gccgcacgtg gcgattcaag ctcagcgctt
    18661 tgtactgcgt cagcacgaac acgcggcgca aatcgctatt caggcaattg gaaagcgtca
    18721 gatcgataat tcggtaaacg ccgccgaaag gcacggcggg cttagcgcga tcgcgcgtca
    18781 acggccaaag ccgctcgcct tggccgccgg ccagcaatac tccgatcgca tcgcgcatcg
    18841 atggcatttt ggaacgcgcc gcagcccttc cctgggcgcc caaacattct agcagcactc
    18901 gttcctggag ttcagcagga cctccttcat gcgcgaaaaa tgagtttccg caaccgccat
    18961 ttacgcgcgc ttcagcgccc ccgcgccgcc gccctaatgt acaatttatg tcttgacttg
    19021 agggatagcc tccgatagaa ggagtgagca atagccgggt tttagcggat ccggttactc
    19081 gatttcgaat ccacaagtcc aattcaaaca gagagacggg ggtgaatacg cgtggcggaa
    19141 gtagttattc aagagggcga gtctttggaa aacgcgctcc ggcgattcaa acgtaaggtc
    19201 cagtcggagg acatcatcaa ggaaatcaag aagcacagtt actacttgaa acctggtgaa
    19261 aagcgtcgag ccaagcaggc tctctcgcgt aagcgtctcc gtaagaagca gcggcgtgag
    19321 ctggattagg gaagccacaa ccggaaaacc gaaagcagtt cgatgccggc gagagggtaa
    19381 gcatcgcggg catccgcgat ttcatccttc gccggttgcg aactgcaaac tgcttgctct
    19441 gtgcgaacac ggcccccgga gcagcgtatg gaatatcatg acaaggttct gaagtgcgcc
    19501 gagtgcgggg ctgaattcgt tttcaccgcc ggcgagcaaa tgtttttcgc ggacaagggc
    19561 ttcaagaacg agcccaagcg ttgcaaggga tgcaagtcgg ttcgcaccca aggcggcgaa
    19621 tcagcggcgc cccccaccca acgggtggaa acgaaaacgg tctgctcgca atgtggaaaa
    19681 gagacgaccg tcccgttcaa gcccacccaa ggccgccccg tttactgccg cgaatgtttt
    19741 cagcagcggc gagcttttgg ggcgtcaagc gcaatggcct gattcttcca ttccgctggc
    19801 gatcctaaag cacttgcatc aaaaagcagc ggcatgcctt tttcaggctc tcgccgatcg
    19861 caccaatcat attttatttg tcaccttaca ggattcgctg tattggactt ctggtaggga
    19921 ctcgccaaaa ttcttagtcg cggaacgttc ggcaacctga ttcctgggtg cctcgattcg
    19981 gagtaactat gagcgcggtc accaaggtgc ccaccgacac atctgccgtc cccggaatga
    20041 gacaaaccgc aagaagccac cacaatcacg tcgtgcaatt ttacggagag gattccgcgc
    20101 ttctggatgc ccttgtcggt ttcatcggcc aggctctgaa ggccggcgat gccgctctcc
    20161 tgatcgccac tgaggctcat cgcgaggaac tcgggcggcg cctcgaagcc gagggaatcg
    20221 atgtgagcgc cgcgacggcg caaggctgct atattccgat ggacgcggcc gcgactcttt
    20281 ccactttcat gatcgacggt atgcccgatc ctatcctctt cgaggaagcg atcggcaagc
    20341 gcgtcctgct ggcgcggaag gctgctcaaa acgagaacgt tcgcttggtc gcgtttggcg
    20401 agatggtggc tattctgctg cggaacggtc agcccgatgc tgctctagct ctcgaacgtc
    20461 tttggacgaa gctcgcgaaa acctactcct tctctttgcg ctgcgcgtat ccgatggaac
    20521 aatttaacag ccagtcgcgc agagagcttt tcatgaaaat ttgcgatgaa cattccggcg
    20581 tgattccggc cggaggatac gccatgctct cgaccgagga cgagcgcctt cgctcgattg
    20641 cggaattgca gcagcgggcc aagtcccttg aggcacaaat tgctctccac cgcagcgaac
    20701 aacagctccg cctgctcgtt gaaaccgtcc aggattacgc tatcttccta ctcgaccccg
    20761 atggtcgagt ggcaacctgg aatctcggag cgcagcgcat caagggctat caagcctccg
    20821 aaattatcgg caaacacttt tccatctttt atcccgagga agatatcaaa tcgggaaagc
    20881 ctcaggaaga actgagaatt gccaccgccg tgggccgctt cgaggacgag ggaggctggc
    20941 gtctccgcaa ggacggctcg cgattttggg ccaacgtcgt cattaccgcc ttgcgcgact
    21001 cctcgggaaa tctccagggt ttcagcaaaa tcacgcgtga tattaccgag aaaatgctgg
    21061 cacttgaagc tctacggaaa accaaccggg agttgcggga agagatttcc aggcggaccc
    21121 tagccgaaga gaaactgcag gagtccgggg ggtcgctgcg caagttatcc ggccatctcc
    21181 tccgcatgca ggatgaagag cggcggcgcc tcggccgcga gctccacgat agcgtcggcc
    21241 aatacctcgc cgctttgaaa atgggcttgg acgccataca cgatggttcc aaccctcaga
    21301 ttcaggaatg cattaacatg gccgatcagt gcatgaagga ggtgcgcaca atatcgtatc
    21361 ttctttatcc tccaatgctc gaggaagtcg gcctgcgctc tgcaataccc tggtatatcg
    21421 aaggtttctc gaaacgcagc ggaattaagg tcgactgcga catcgaccca aaggtcggac
    21481 gcctttctcg cgacgccgag ctggcaattt tcagaatcct tcaggaaagt ctgaccaacg
    21541 tccatcggca ctctgaaagt cccacagcga ccgttcgctt gatgcttgca gacagtacgg
    21601 tttacctcga agtgagcgac cgggggaagg gcattcctcc ttccgcgctg gagcccggtg
    21661 gcgacgcttc gaacactttg ggagtcggcc tccgcgggat gatcgaacgc atgcatcaac
    21721 tcggaggtac gatggaacta tcttccaatg aacgcgggac caccgtgcgc gccagcctgc
    21781 cgcgcgaaaa ggtgtaaact ttgttttggt gctggaaaac gcgtaggaaa acgcagttcc
    21841 acacattggc cgccgagttt cttcaagcga agtccagccc cagcacgaaa gcgacgcacg
    21901 gcctcgaaat ggagctcaaa tgccagccag aattctagta gccgacgatc acgaagttgt
    21961 ccgccagggt gtgcgggcca tgattcaccg cttgcgtccc aaatgggaaa tctgcggcga
    22021 agcggctagt ggagacgagg ctcttcaagc catactgtct ctcaaacccg aggtcgccat
    22081 cctggatatc accatgccgg ggatgagcgg cctcgacgtt gcgtacgaag tcgccaagcg
    22141 ccatctaccc accgcgctac taatcttcac catgcacgaa tccgagagaa tcgcgggcga
    22201 ggtccgcgag gcgggtgctc agggattcgt caacaaatct tgcgctggac gcgatctcat
    22261 cgccgcgatt gaggcgctcc tgggcggcgg cacgttcttc ggcaggccca tcccagccgg
    22321 cacgcccgaa gggggcggtg gcgagcccaa cccgcggctc ttgtttcgtc ggcaactcac
    22381 cctcttccag acctgaaagc ctcgtggcgc aggcactctt gcgtgcgcgc ctaagccggg
    22441 acaaatccgt aagccacagt tacggttgtc atcctgagcg gagtcgcgcg tcgcctttgc
    22501 ctttcccgcg ttttttgcgg gtgcgcgaca cagtcgaagg acctcccttc gatttcgccg
    22561 ccgactccgc gcccgctgtc agccgtcgta gtggaggtgc caccagcaaa ggtgacccac
    22621 ttccgaagtt cccttacctc ctcgcatcgg ccgcaaggta ggattggtcc cgacgcgtcc
    22681 atgagactca ctgcaaaatt tttctcgtgc cttctcgcct tgtcgctcct cgcgatccca
    22741 gcgtgcgccg ctggaatcgt gctttcaccg gaagccgcca aaacgctcga tgaaatttac
    22801 aacggcgata ccgatgccgc cgtcgagacc gcgcacggta tcgagcaagc tcagccggat
    22861 agccctgtgg gctatcttct cgatgccgaa gcgcagtggt ggaagatgta ttgcgccgca
    22921 tgcgaaatca aatggggcat gctcgacgct tggaagcgcg ggaaaaagcc ggaagacgcg
    22981 tcctacttcg cattggcgga caaagcgatt gaattagcgc gcgcgcaaat cgccaaatca
    23041 gacaccgccg aaatgcacgt ttatgttgct atcggcttcg cgctcaaggc ccgcctctac
    23101 ggtttgcgcg atcagaagcg cgacatcgcc cacgctggcg ttgccgctcg gtccgaatgt
    23161 ctgcgcgcct tgcagctcga tcccgatatg gctgacgcta cggctggcct gggactttac
    23221 aactactatg tggacaccct ttcaggcatc gccaaggtgc tgcgcttttt catgggcatt
    23281 cccggcggag acaaacagga aggaatccgt caaatgaaaa tcggcatgga gcgcggcgca
    23341 ttcctcgccg ttgactcgcg cttctatctc gcgaaaaatc ttcgcagcta cgaccgccat
    23401 tacgatgaag cgctagaaat agccgagccg ctagccacgc gctatccgcg caattccatt
    23461 tttcttctgc tgctcggaaa cttgaatcag gaattgggac gcaacgacaa agcctcggaa
    23521 tattctcgcg ccgcgcaatc cgcgcccatt gccgacccag cctgcgccaa ccgcgtccgc
    23581 gacctcgcca actccctcct cgcctctccc cactgagcca tctcgcatgt cgccggtttc
    23641 atgcatcatc gccccatcgt cgttccgagc gaagcgtgcc gacggttcca tgtagctcac
    23701 gccgccttca tggcgtgagg ccgttcctcg tctcgcgctc tttgcttatc gcggagcatg
    23761 atcctcaaat cgtcattccg agcgaagttc cgcgcttttt ctcttcgcgc gtcgcgggag
    23821 gatcgaatca gagaaccacc gtcattctgc ctgccctgac gcaggaaggg agcgaagcgt
    23881 gccgacgatt tttcttcgga gatcggttcc cagatcttcg tcggatgcgc atcctggcga
    23941 cgttgttttt gtaggggcac ggcatgccgt gcccggcaaa cctacttgcc tacctgctcg
    24001 ccatccggct cccgtaaaca ggcgccgtca tcctgagcgg agtggcgcgt gtctttgctt
    24061 ttgcgcgaag cgccgcgcca cgcagtcgaa gggcctctct tggatataag acccacctcg
    24121 ggctcccttg cgccctcgcg tactcggcga acccttccct ctccgctgag ttgacaccgc
    24181 ccagcacgcc agctacgctc tccggctatc aaagcttcgc tgcaaagcac cgcggatcgt
    24241 gaaagctcca ggatagcgaa tgaaaaatac gctcaccgct ctgattggcg ctgttctgat
    24301 gtttgccgtg cctcctgcaa ttgctcacca ttctcgatcc atgttcgatc aatcgcggca
    24361 gatcacgctc gccggcactg tgaaagaatt ccagtggact aacccccact gctggattca
    24421 ggttctcgtg cccgacccga acaatccgca agcggctccc atcgagtggg gcgtcgagat
    24481 gggcgctcca gtcgaactga tgcgcaaggg gtggaagccg cgaagtctga atcccggtga
    24541 caaggtcacg atcgtcgtta atcccttgcg cgacggccag cacggcggtc tcgtcctcgc
    24601 tgtcaccggt ccagacggca aaattatcgg aaggcccgcg atccccgtga aatagtcgcg
    24661 cgccggagga ggccccgcca tgaagcgtca agcattagcc gcaatggtga ccgtgctgct
    24721 cgcaggatat ttctgcgcgg tgcacgcgca aacttcttcg aaacagcgcc tcgccgcctt
    24781 tgccaaattg cccgattggt cgggcatctg ggaactcgat gctttcgtcg ggcagtccga
    24841 cggccagcaa tttagtccgg aaggtcagcg cgaattgaag gcgtatgcgg cggccctgcg
    24901 gccttctttc actcccgaat atcaggccaa gtacgatgcg attcgaaaaa aggtcgctga
    24961 cgccgtcgcc gccgatccgg cccacccgcc cgccacgcat cagccgctgt gtgtcgctcc
    25021 gccgttcccc ggaacctctt cgccgggaat gtttgagtgg ctggtgacgc cggaggaaac
    25081 cactttcgtc agcacagtgg gcgccgtccg gcacatttac accgacggcc gctcgcatcc
    25141 tccgaaggac gagatgtggc ccacgctgat gggcgactcg atcggccact gggaaggcga
    25201 cacgctcgtc gtcgatacca tcggcacaag aaagcgcctg tacacgggcg agctttccgg
    25261 attctttttc ccgatgagcg atcagctcca tttcaccgaa cgcatccgca tggtgaatca
    25321 caacgaaatg cagatcgact acacggtcga agacccgatc gcgctaacca agccgattca
    25381 cgcgacgatt actcacaccc gcgtcaccga cttcaaccgc atgatcgaag aaaccgattg
    25441 cgagcagaac gaccgcgatc ccatcgtcaa cggccgcttc accaccgtca cccattaagt
    25501 cttatatctt tgtctttgct taaggtttag acgtagcgcc gggttcttca gcccggcagc
    25561 cttcgcttcg aaggttcaaa cgtagcgcct ttgcattcgt aggggcacag catgctgtgc
    25621 ccggcaaaca tgcttggcta cttgctcgcc atccgcctca ttggacgcgt gtcattccgc
    25681 ttgccctgac gaaggaaggg agcgaagtgt cccgcgtctt ccacgcgctg cggattcggt
    25741 cattccgagc gaagtgtccc gcgctttgct tttgcgcgaa gcgccggaac acgcacggaa
    25801 gggctcccgg gtcgacggtt agtcgagccc gggagatcag acgaatctct cttcgatctt
    25861 ccaatcaact acgcgccaac cacgcgcggc tttttcctgc tcgaaggcgt gatacgagga
    25921 tgcaccagcg gatgagcttc cacatgctgc gaattcgtca ttccgagcgg agcatgccga
    25981 cgatttttct tttcggagat cggttcccag atctccgtcg gcatgcgcgt cgagaaatct
    26041 ctcttcgatc gcccaatcaa ctacgcgtca accacgcgcg gcttcttcct gttcgaaggc
    26101 atgatacgag gaacgcacca gcggtccggc ctccacgtgc ttgaatccca tccgctcgcc
    26161 cagccgcttg aattccgcaa actcatccgg atgcacgtac cgctcgatcg gcaaatgttc
    26221 cggagtcggc tgcatatatt gcccaagcgt caaaatgtgc gtgccctgcg cggcaatctc
    26281 ttccatcgcc gataaaactt cctcgcgcgt ttcgccaagg ccgagcatca tgcctgtttt
    26341 agtcggcgtc tcgggcgcca tttctctcgc gcgccgtagc agctcgagcg atcgttcgta
    26401 caacgctccc ttgcgcacac gacgataaag ccgcggcacc gtttccatat tgtgattcag
    26461 cacatccggc cgcgcggcca tcacttttcc cagcgcgtcc caattgccgc gaaaatccgg
    26521 aatcagcact tccactttgc agtccggcac tcttcggcga atttccgcga tcgtgcgcgc
    26581 gaaaatttcc gcgccgccat ctggctgatc gtcgcgattc actgatgtca cc
//
LOCUS       DATNDT010000528         7492 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1379803, whole genome shotgun sequence.
ACCESSION   DATNDT010000528 DATNDT010000000
VERSION     DATNDT010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 7492)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7492)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7492
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379803"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..695)
                     /locus_tag="VN788_17230"
     CDS             complement(195..695)
                     /locus_tag="VN788_17230"
                     /inference="COORDINATES: protein motif:HMM:NF015648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HXU22324.1"
                     /translation="MTDIVIRPTMKRIKAGYLLIAILVAAGLIAEHIFAPDKPWWWAA
                     IGAVLFLWPLSKHIQRRGAGRLTMSADRLRFESGVLSKSTRTVQLLKVQDVRIKQSFF
                     QRMWNIGDLSIASAGDPELFQIDNIDRPRAVSEKIVDRCQFVKSQLPAESPAMPANTP
                     PEAPRG"
     gene            764..1453
                     /locus_tag="VN788_17235"
     CDS             764..1453
                     /locus_tag="VN788_17235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012435785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HXU22325.1"
                     /translation="MRILIVEDEKRIQDFLSRGLESAGYAVDVAGDGATANELAHNTE
                     YDLVILDLMLPDTDGLSVLQKIRNRKASPPVLILSARDTVDDRVKGLELGADDYLVKP
                     FAFVEMLARVRALLRRGQPTPERLQVGELVLDCIRRKVVREGENIDLAPKEFSILEYL
                     MRNRGRPLSRTMIVEHVWDMDYDGLTNIVDVYIRHLRSKIDDRFPDKMIHTVRGIGYM
                     LDTPEKMSAKT"
     gene            1473..2957
                     /locus_tag="VN788_17240"
     CDS             1473..2957
                     /locus_tag="VN788_17240"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HXU22326.1"
                     /translation="MEVTRRAVRFSRSLRFRLAVSYALAFSLLLVLLGLAFRPTLRNI
                     MDNRAAETLNEEWGAVHGYLYFPKYGPDFFYDAFDPEEGLIVSRLKSIFFMADAQGNP
                     VLVSDSFKTIGLPSKQEIESTLASGQTSIQIRKGKDGIPYMIRIGRIVDEKPKPQRVY
                     FMAIGRSLADDRRILDRFARDYFLLLPVLIALCCGFGWFIAGRALEPLNSVSRTALRI
                     TSSKLGVQIPLRGADDELDHLIRAFNRMMERLNRSFEQIRQFSTDVSHELRTPLTVVR
                     GQLEVALFTAKTTEQYREAMVNALEDVERLSSIVRALLLLSQSESGQLVLHKATFDLA
                     ETTRDLVDQFQIPAQEREIRLEAEAPETCLVNADRIQIERLLSNLLSNAIKYTQQGGW
                     VRATLRQLENKVELVVADDGAGIAPEHLPHIFDRFYRVPSFEPEKGLGLGLSFVAWIV
                     KAHDGKISVDSAPGNGSRFTVILPSGLPTPASQPSTLTGAEKIH"
     gene            2977..3369
                     /locus_tag="VN788_17245"
     CDS             2977..3369
                     /locus_tag="VN788_17245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HXU22327.1"
                     /translation="MTGREREGITIFDLSGRLIVGEPAQIVRESILEALKEEKKNVIL
                     NLEEVEYIDSTGLGSMVICYTTLQKAGGALKLLKLNKRNIELLVLTKLSTIFEIFNEE
                     QDAVNSFFPEREIKRFDILSFVQQQKDQ"
     gene            3379..3846
                     /locus_tag="VN788_17250"
     CDS             3379..3846
                     /locus_tag="VN788_17250"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HXU22328.1"
                     /translation="MLRSGAQAPSFQLPDPDGKSRSLDEMLAEGKPLLLVFFKVSCPV
                     CQLIFPFLERISNSEALRFVAISQDDAATTRNFHQRFGVTFPTLLDSVRAGYPASNAF
                     QITNVPSLFLIEPDGTISLAIPGFSRRDLEALGQRAGAKPFHAGDQVPEWKAG"
     gene            3923..4429
                     /gene="nuoI"
                     /locus_tag="VN788_17255"
     CDS             3923..4429
                     /gene="nuoI"
                     /locus_tag="VN788_17255"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HXU22329.1"
                     /translation="MASKVGEVFQSAAAIAKGMSVTFKEMMQPTITEDYPDTPPKFQE
                     RYRGVHVLQRDENGLEKCVACFLCSAACPADCIYIEAAENTDEMRISGGERYAKVYNI
                     DYNRCIFCGYCVEACPTDAITHGHGFEIASFNTSTLVYRKEQLLAPIPPGANFPLKAE
                     PETAVAGH"
     gene            4477..5235
                     /locus_tag="VN788_17260"
     CDS             4477..5235
                     /locus_tag="VN788_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714990.1"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HXU22330.1"
                     /translation="MNGNNQNRLGGAAVLGALLAVGLILGGWVVGSQIKSTRLSDRYV
                     TVKGLVERTVKSDLAIWPLSYKEAGDDLSALYAKTENDKKTILDFLDHQNIKPSEIEL
                     GVIRVVDTQANEYGGNTRAPHRYIVEQQITVRTPQVDRIAAAAQKTMQLLQKGIVLTS
                     NPGQGLAYKFMGLNSIKPDMITEATRNARAAAARFASDSGSKVGSIRQANQGFFSILP
                     TDQASETSEGNFSADSSLMKTVRVVTTVQYYLDQ"
     gene            complement(5273..5554)
                     /locus_tag="VN788_17265"
     CDS             complement(5273..5554)
                     /locus_tag="VN788_17265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22331.1"
                     /translation="MKLARRIVAIALLFVAVLYACDYLSVRYHIPHNRNPLGVVKVQP
                     YYAVPLKNKKTELMFLDPQEETCVHSLFPHLGYRPCWYVSRQSKKRIDM"
     gene            complement(5551..5922)
                     /gene="queD"
                     /locus_tag="VN788_17270"
     CDS             complement(5551..5922)
                     /gene="queD"
                     /locus_tag="VN788_17270"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HXU22332.1"
                     /translation="MFEVCVENTFAAGHALRNYHGKCENVHGHNYRVRMTMEGEQLDA
                     TGLLVDFVEIKRLMSAIIDKLDHQFINDIAPFDVINPSAENIAKYFYDEIYSGLKTET
                     PVRISEVKVWETDVSAAAYRP"
     gene            complement(5915..>7492)
                     /locus_tag="VN788_17275"
     CDS             complement(5915..>7492)
                     /locus_tag="VN788_17275"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HXU22333.1"
                     /translation="RQKIYDAEREPVLQGLEEIADFQIIREWEKSTETGDRAEIKTLP
                     EHSSTWGKIDARRDLCTGQRCQQFERCFLTLMHQKAHESDIIIVNHHLFFADLAVKED
                     DFGGIIPEYGAVIFDEAHEIEDVAGQYFGVSISNYQIEDLARDIAGISRKKQFGSEEL
                     DRVLQVLRECSAIFFACFGDTDGRAGFREHAAFLETHGEDFDDLLRALELTVSHLQLV
                     KESPEEVFPLMRRIGVIAQNLSLWMSGKERAYVYWVERRGRGCFLQATPIDVSSVLAE
                     KLFDRIDTVILTSATLAVGGNFDYAEQRLGLRGARTLIVPSHFDYPKQALLYVPQYLP
                     DPRNPAFTRAAGDEIARVLRASAGRAFVLFTSYREMRLIHDRLSLELEYPALLQGTGP
                     RSALLEEFRRTPNCVLFATSSFWQGVDVQGEQLSCVIVDKLPFAAPTDPVVEARIQNI
                     RESGGNPFYEYQIPRAALVLKQGFGRLIRSRSDRGALVLLDNRITKQRYGQVFFDSLP
                     DYGFTTEFEDIERFFDV"
BASE COUNT         1644 a         2027 c         2238 g         1583 t
ORIGIN      
        1 caaatggcgg acgtgttgcg cggccacatc gcaacgccgc cgagtccgtt gccgccggtg
       61 agttcgaact caccgggcca gccgcgctga ggggcggcgg cctatcctgg ccgcgaacca
      121 gccttctggc cggctcaagc ctgctaaaag caggctgcag gctgaaaagc ctgccccaca
      181 ttgcagcggc gcgctcaacc gcgcggcgct tcgggtggag tgttggcggg cattgcggga
      241 gactcggcgg gcaattggct cttgacgaac tggcagcggt ccacgatttt ctcggaaacg
      301 gcgcggggac ggtcgatgtt gtcgatctgg aacagctccg ggtcaccggc gctcgcgatt
      361 gataaatcgc ctatgttcca catgcgctgg aaaaaagact gtttaatccg gacgtcctgc
      421 actttcagaa gctgaaccgt gcgcgtggat ttcgataaaa caccgctttc gaagcgaaga
      481 cggtccgcgc tcatcgtcag ccttccagcg ccgcgccgtt ggatgtgctt actcagcggc
      541 cagagaaaga gaaccgctcc gattgcggcc caccaccagg gtttgtccgg ggcgaagata
      601 tgttccgcga tgagaccggc ggcgaccagg atcgcgatga gcagatatcc cgccttgatc
      661 ctcttcatcg tgggacgaat tacgatatca gtcatagagc cgccaccacg cattgtacag
      721 cggcgcgcgg gagagccggt gttaaactga ccttcgaacg cctatgcgca ttctaatagt
      781 agaggacgag aagagaatcc aggactttct ttcacggggg ttggagagcg cggggtacgc
      841 cgttgacgtc gcgggcgacg gcgcgacggc gaacgaactg gcgcacaaca ccgaatacga
      901 cctggtgatt ctcgacctga tgttgcccga tacggacggg ttgagcgtcc tacagaaaat
      961 tcgcaaccgc aaggcgagcc cgccggtcct gatcctgagc gcgcgcgaca cggtggacga
     1021 tcgcgtgaaa ggcctggaac tcggcgcgga cgattacctg gtgaagccgt ttgctttcgt
     1081 ggagatgttg gcgcgagtgc gggcgctgct gcggcggggc cagccgacgc cggagcgctt
     1141 gcaggtgggc gaactggtgc tcgattgcat ccggcgcaaa gttgtgcggg agggagagaa
     1201 tatcgacctg gcgccgaaag agttcagcat tctggaatac ttgatgcgga atcgcgggcg
     1261 cccgcttagc cgcacaatga ttgtcgagca cgtctgggac atggattacg atgggctgac
     1321 gaacattgtg gatgtctaca ttcggcactt gcgcagcaag atcgacgacc gatttccgga
     1381 caagatgatc cacacggtgc gcggcatcgg ctacatgctg gacacgccgg agaagatgtc
     1441 cgcgaagacc tagaacgggc aaggcccgag ccatggaagt tactcgccga gcggttcgct
     1501 tttcacgcag tctgcgcttc cgtctcgcag tcagctacgc tctcgcgttc tcgctgttgt
     1561 tggttttgct gggactggcg tttcgcccca cgctccgcaa cattatggat aaccgggccg
     1621 ccgagacgct gaacgaagaa tggggggcgg ttcacgggta tctctacttt cccaagtacg
     1681 ggccggattt cttctacgat gccttcgatc cggaagaagg gctgatcgtc tcacgtttga
     1741 agagcatatt cttcatggca gatgcacaag ggaatccagt gctggtgtcg gattccttca
     1801 agacgatcgg ccttccgtcc aagcaggaga tcgagagcac gctcgcgagc ggacaaacga
     1861 gtattcagat ccggaagggg aaggacggaa ttccgtacat gatccgaatc ggcaggatcg
     1921 tggacgaaaa accgaaaccg cagcgtgtgt acttcatggc gatcgggcgg tcgcttgccg
     1981 atgaccggcg tatcctggac cgattcgcgc gcgactattt tctgctgctt ccggttttaa
     2041 tcgcgctgtg ctgcggcttc gggtggttta tcgcgggacg agcgctggag ccgttgaatt
     2101 cggtttcgcg gaccgcgctg cggatcacca gctcgaaact cggcgtgcag attccgctgc
     2161 gcggggccga cgatgaatta gaccatttga tccgagcttt caatcggatg atggaacgat
     2221 tgaaccgctc gtttgaacaa atccgccaat tttcaacgga tgtctcgcac gagctgcgga
     2281 ctcccctgac cgtggtgcga ggtcagcttg aagtggcgct gttcacggca aaaacgacgg
     2341 agcaataccg agaggcgatg gtaaacgcgc ttgaagacgt cgagcggctt tcgagcatcg
     2401 ttcgcgcgct gctgctgctt tcgcaatcgg aatcgggcca gcttgtgctg cacaaagcga
     2461 cgttcgatct cgcggagacg actcgagatc tggtggatca gtttcagatt ccggcacagg
     2521 agcgcgagat caggctggaa gccgaggctc cggaaacgtg cctggtcaat gcggatcgca
     2581 ttcagatcga gagattgctc tcgaacctat tgtcgaacgc aatcaaatac acgcagcaag
     2641 gcggctgggt gcgcgccacg ctccgtcaat tggaaaataa ggtggaactt gtggtcgcgg
     2701 atgatggggc cggtatcgcg ccggagcact tgccacacat cttcgaccgg ttctatcgcg
     2761 tgccttcttt cgagccggag aaaggacttg gcctgggcct gagtttcgtc gcgtggatcg
     2821 tcaaggcgca tgacgggaag atttccgtcg acagcgcgcc tgggaacgga agccggttta
     2881 ccgtgatcct accttccgga ctaccgacgc ctgcttctca accttcgacg ctgaccggcg
     2941 ccgaaaagat acactagggg cgtggcgaac ctcgaaatga ccggccgcga gagagaaggc
     3001 atcacgattt tcgatctcag tggccgcctg attgtcggcg aaccggctca gattgtacgg
     3061 gaaagcattc tggaggctct gaaggaagaa aagaaaaacg tgattctcaa tttagaggag
     3121 gttgagtata tcgacagtac cggcctcggc agtatggtga tctgctacac gacgctgcag
     3181 aaggcgggcg gcgcgctcaa gctgctgaag ctcaacaaaa ggaatatcga gctgctagtg
     3241 cttacgaagc tgtcgaccat ctttgagatc ttcaacgaag aacaggacgc cgtaaacagc
     3301 ttcttccccg aacgggagat caagcgcttc gatattctca gtttcgtgca gcagcagaag
     3361 gaccagtaac gcgggaagat gttgagatcc ggcgcacagg ctccttcgtt ccagttaccc
     3421 gatccggatg gcaagtcccg ttctctggac gagatgcttg ccgaggggaa gccactgctg
     3481 ctggttttct tcaaagtaag ttgcccggtg tgccagctta tttttccgtt tctcgaacgg
     3541 atatcgaaca gcgaggcact gcggttcgtc gcgatatcgc aggacgatgc cgcaaccacg
     3601 cgaaattttc atcagcggtt tggcgtcacc tttccgacgc tgcttgatag cgtgcgggct
     3661 ggatatcccg ctagcaacgc gtttcagatc acgaatgttc cgtcattatt tctgattgag
     3721 ccggacggca cgatttctct cgcgattccc ggcttttcga ggcgggatct ggaggcgctc
     3781 gggcagcgcg ccggcgcgaa accgtttcat gcaggcgacc aagtgccgga atggaaagcg
     3841 ggttgaggat cgaagaactg agctcccggc gggggcttac gatgatgcca ggatgattcg
     3901 ttgaggtaag ctaaaaacag acatggcttc caaagttggc gaagttttcc agtccgcagc
     3961 cgcgatcgcc aaaggcatga gcgtcacctt caaggagatg atgcagccga cgatcacgga
     4021 ggattatccg gatactccgc cgaagtttca ggagcggtat cgcggcgtgc acgtcttgca
     4081 gcgggatgaa aacggcctgg agaaatgcgt ggcgtgtttc ctgtgctcag ccgcgtgtcc
     4141 cgccgactgc atttatattg aagccgctga gaacacggac gagatgcgga tcagcggcgg
     4201 cgaacgttac gcgaaggtgt acaacatcga ctacaatcgt tgcatcttct gcggctattg
     4261 cgtggaagcg tgtccgacag acgcaatcac tcacgggcac ggcttcgaga ttgccagttt
     4321 caacacttct acgctcgttt atcggaaaga acagttgctc gctccgattc ctccaggcgc
     4381 gaacttccca ttgaaagcgg agcccgagac ggctgtggcg gggcactagg caaggaccga
     4441 ggccgaagat cggttttagc gcgccgagga gggccgatga acggcaacaa ccaaaacaga
     4501 ctgggcggcg cagcggtgct gggggcgttg ctggcagtcg gtctgattct cggcggttgg
     4561 gtcgtgggat cgcagattaa gtcaacccgg cttagcgacc gctacgtgac cgtcaaaggt
     4621 ttggtagagc gcacggtcaa gtccgatttg gccatatggc cgcttagcta caaggaagcg
     4681 ggcgacgatt tatccgcgtt gtacgcgaag actgagaatg acaagaagac cattctcgat
     4741 tttctggatc atcagaatat taaaccctct gaaatcgagc tgggtgtgat tcgtgtcgtg
     4801 gatacccagg ctaacgagta tggcggcaat actcgcgctc ctcaccgcta tatcgtcgag
     4861 cagcagatta ccgtacgaac tccacaagtc gatcggattg cggctgccgc gcagaaaact
     4921 atgcagttgc tgcagaaagg gattgtgttg acgagcaatc ccgggcaagg attggcttac
     4981 aaatttatgg ggttgaattc gatcaaacca gacatgatca cggaagctac gcgaaatgcc
     5041 cgcgccgccg ccgcgcgctt cgcctcggat tcaggcagca aggtgggatc cattcggcag
     5101 gccaatcagg gttttttctc cattttgccg acggaccagg caagcgaaac cagcgaggga
     5161 aatttctcag cggacagcag cctcatgaaa acggtccgcg tagtcacgac ggtgcagtat
     5221 taccttgacc agtagagatg acgcttgcgc gcgcttttgc ggtttctgta agtcacatat
     5281 cgatccgttt tttgctctgt cggctcacat accagcatgg cctgtagcca aggtggggaa
     5341 aaagagagtg aacgcaggtc tcttcttgcg gatcaagaaa cataagttcc gtctttttat
     5401 tttttagcgg gactgcgtaa tacggttgga ctttcaccac tccgagcgga ttgcggttgt
     5461 gaggaatgtg gtaacggacc gagagatagt cgcaggcgta cagaacagcg acaaacagca
     5521 gcgctatggc gactatgcgc cgcgcgagct tcatgggcga taggcggccg ccgaaacatc
     5581 cgtttcccag accttcactt cggaaatccg gacgggcgtt tccgttttta agccggaata
     5641 aatctcgtcg taaaaatact tcgcgatatt ttccgctgac ggattgatta catcgaacgg
     5701 cgcgatgtca ttgatgaatt gatggtccag cttgtcaatt atggcgctca tcagacgctt
     5761 gatttcgacg aaatccacca gcaatccggt ggcgtccagt tgctcgcctt ccattgtcat
     5821 gcggacccgg taattgtggc cgtgaacgtt ctcgcacttc ccgtggtaat tgcgcagggc
     5881 gtgaccggcg gcgaaagtat tttccacgca gacttcaaac atcaaaaaac ctttctatat
     5941 cctcaaattc cgtagtgaat ccataatccg gcagactgtc gaaaaatacc tgcccataac
     6001 gctgtttcgt gattcgatta tccagcagga ctagcgctcc gcgatcggag cggcttcgga
     6061 tcaggcggcc gaatccctgc ttcagcacca gcgccgctct cggaatctga tactcataaa
     6121 aaggattgcc gccggattcc cgaatgttct ggatccgcgc ttccactacc ggatcggtgg
     6181 gtgcggcgaa cggtaactta tcgacgatga cgcagcttaa ttgttcccct tgcacatcaa
     6241 caccttgcca gaatgacgat gtcgcgaaca gcacgcagtt aggcgtgcgg cggaattctt
     6301 cgagcagggc gcttcttggt cctgtccctt gcagcagggc cggatattcg agttcaagcg
     6361 acaggcggtc gtgaatcagc cgcatctccc gatagctggt gaagagcacg aaggcccggc
     6421 ccgcgctcgc ccgcaaaacg cgcgcgatct cgtcgccggc cgctcgcgtg aatgccggat
     6481 tccggggatc gggaagatat tgcggcacgt ataacagagc ctgcttcgga taatcgaaat
     6541 gactcggaac gatcaacgtc cgggcgccgc gtaacccgag gcgctgttcg gcataatcga
     6601 aattgcctcc aacggcgaga gtggccgagg tgaggatcac ggtatcgatg cggtcgaata
     6661 gcttctccgc gagcacggag gagacatcga tcggagtggc ttggagaaaa cagccgcgcc
     6721 cgcgcctttc cacccagtac acataagctc gctccttgcc cgacatccac aagctgagat
     6781 tctgcgcgat cacgcctata cgccgcatca gaggaaacac ctcttcgggc gattctttta
     6841 ccaactgcaa atggctgacg gtcagttcca gcgcgcgcaa caaatcgtcg aaatcttcgc
     6901 cgtgagtttc aagaaatgca gcgtgttcac ggaagccggc gcgcccatcg gtgtcgccga
     6961 agcaagcgaa gaaaatggcg gagcattcgc gtaaaacttg caagacccgg tcgagttcct
     7021 ccgagccgaa ctgcttcttg cgcgaaatgc cggctatgtc ccgggcgaga tcttcgattt
     7081 gataattgct aatgctgaca ccgaaatact ggccagcgac atcctcaatc tcgtgcgcct
     7141 catcgaaaat gacagcgccg tattccggaa tgatgccgcc gaagtcgtct tccttcaccg
     7201 cgaggtcggc gaagaagaga tggtggttca caatgatgat gtcgctctcg tgcgctttct
     7261 gatgcatcaa cgtcagaaag cagcgctcga attgctggca gcgctggccc gtgcagaggt
     7321 cgcgccttgc gtcgattttt ccccatgtgg agctgtgctc tggaagcgtt ttgatctcgg
     7381 cgcggtcgcc ggtttcggtg gacttctccc attcacggat gatttgaaaa tccgcaatct
     7441 cttcgaggcc ttgtagaacg ggctcgcgct cggcgtcata gattttctgg cg
//
LOCUS       DATNDT010000529        16460 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_180468, whole genome shotgun sequence.
ACCESSION   DATNDT010000529 DATNDT010000000
VERSION     DATNDT010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 16460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16460
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_180468"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..242)
                     /locus_tag="VN788_17280"
     CDS             complement(<1..242)
                     /locus_tag="VN788_17280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009342224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HXU22334.1"
                     /translation="MTNHTIHFGTSGWRGVIAEDFTFSGVRRASRAIAGHLRAHTKAP
                     VVIVGYDTRFFSEDFARTSAAVLRSDGCRILLCGAPT"
     gene            322..732
                     /gene="cutA"
                     /locus_tag="VN788_17285"
     CDS             322..732
                     /gene="cutA"
                     /locus_tag="VN788_17285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017318775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HXU22335.1"
                     /translation="MTNKIVVLVTCGSIKEAQQIGRALVDRKLAACANILHAPVHSIY
                     RWKGKVDVAKEILLILKSSRSRFAAIDRAIRNMHSYDVPEIIAIPIAAGSRSYLDWIS
                     KSVSAGQENVALKRATHQTSILRDVGDRSKKRAS"
     gene            729..1223
                     /locus_tag="VN788_17290"
     CDS             729..1223
                     /locus_tag="VN788_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HXU22336.1"
                     /translation="MKAKRRTRAGFGWIIYPALLAICLLCVPQELAQTDNSSQPPASD
                     QSSATKQDQSSAADKKEQTPSESATARLRIIVTTGEDKPIGNASVYVRFNTPGGFLRH
                     EKQVEMNFKTNQDGSVKVPDIPQGKILIQVIAKGWHTYGKWYDVQKEEETIAIKLAAP
                     PHWY"
     gene            1626..2492
                     /locus_tag="VN788_17295"
     CDS             1626..2492
                     /locus_tag="VN788_17295"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HXU22337.1"
                     /translation="MKELILNADDFGLTQGINRGIICAHRDGILTSTTLMATGPAFDH
                     AVEQARANPTLSVGCHLVLTGGSAAAPREQIPSLVDKNGRLPRSLTVLVASITTGKVP
                     PIEIETELRAQIEKIRRAGLEPSHLDTHKHTHVHPTIMNALGRVAQQSGIRRVRNPVE
                     DLRDSWRSNRSDGTNAVRNLAAAATVSAVASGFKAISSKYGLRSPDHFLGLAITGQLG
                     AAALCRLIGALDDGQTEIMLHPGICDQDLARTGSRLQRQREVEMEGLLAPEVKDAIVK
                     GGIRLISYRDLN"
     gene            2627..3040
                     /locus_tag="VN788_17300"
     CDS             2627..3040
                     /locus_tag="VN788_17300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22338.1"
                     /translation="MKIMEHRVRALLYVAVIVGVYAVASTVFGQTAKSQYDGSYRGAY
                     SGSFASNSVMGSVEFSVANGVITVTEPGRGAGTVDSSGAATFSGSLGVARVTCKFTGA
                     FRSSTRAQQSVHATGSWSCTGSGQTGKGTWSADRP"
     gene            complement(3111..4463)
                     /locus_tag="VN788_17305"
     CDS             complement(3111..4463)
                     /locus_tag="VN788_17305"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HXU22339.1"
                     /translation="MAEKAQHFAAIVGAAISGSVAAEILAERGIQVAVIEQNKRPYGK
                     IEDGLPRWHVEQRKQEYARIDARLRKPGVHFIPSTKVGRDFDFQELCHEWGFSAVILA
                     NGAWRDRDLGVPGADQFINKGLIYQNPFIYWYNHKNEKGYAGPLYETPDETIVVGGGL
                     ASIDVVKVLQLENYERAMRARGIATNMHELEKGIPAACKAHGIKPEELGVKGCLLIYR
                     RREQDMPLAQPPDNATAEQITKTEQVRQKMLRLAREKYLFRVQDRRLTTALIVEDGRV
                     VGLKVAETKIEGRSAQPIPGSEQELRAPLVISSIGSVPEIIPGVSMKGEYYTFSGEDL
                     PRYSGCEHVFGVGNVVTGQGNIRVSLIHSQRVTRHLIENYIGIGDGVGDLSALYEAAE
                     TQAAEQAEAVEKTIRSAPALSSEKLADIKKRIRTLQEKAGYTSDYDSWIAKVTPPDLE
                     "
     gene            complement(4575..7796)
                     /locus_tag="VN788_17310"
     CDS             complement(4575..7796)
                     /locus_tag="VN788_17310"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HXU22340.1"
                     /translation="MAIKTSTVRPDSLRSESDPGPSKTSGHRSLRDCESNFELLFSTN
                     PLAMYVCDRETLEFLEVNSAAEKQYGYTRQEFLKMKITDIRPAEDIPRLLDIARTNGP
                     SLAFLGQWRHRRRNGEIFDVEVTAQGTTFAGRDGVLVVAQDISVRRAMEEKTAERTAY
                     AQALTENNPTAIVAVNMERRVEMCNPAFERLFGYSLAEIRGAELDSLIAPEDLHREML
                     GLMGRAVSGDIVRATTKRKRKDGSCVDVQVIGVPLIVDGKRTGTFGMYEDITEKKQAE
                     EALHRAEEKHRRIFDNAVEGFFESTPAGRFLVVNPAMARIAGYASPAEMISEIHDIGR
                     QIYTDPSVREEVKRRLEQRGVLEEFECAMLRKDGSKIWISLNVRAVRDASGKIVSHDG
                     TAEDITARKRSQLERQVNTEIVHAVSATDNLDDLLRLIHAALKQVLDAENCFVALHER
                     STGMFHYSFFIDQFDPPPPPDTMDRSCAAYVFRNARGMLIPREVFRDLARRGEVELVG
                     TPSASWLGVPLRTPTETIGVLVVQNYSIENAYTERDLEFLSSVGDQIALAIERKRGEE
                     RVRQSEARLRVLIEQLPAVLWTVDSNLRFTSAVGAGLIRLGLKPDQIVGMSLTEYFET
                     SDPTFLPIAAHRRAVVGEAVTFPVEWKDGSYACHVEPLRDGDGAVHGAICMALDVTDR
                     KKLEEQFRQAQKMEAVGRLAGGIAHDFNNLLMVIQGYADLLAERLPEGEALRRNAEQI
                     RAASQRAAALTQQLLAFSRKQILAPKVLNVHAVVTDLEKILRRVIGEDIELRTSSTAD
                     LWLTKADRSQIEQVIMNLAVNARDAMPTGGRLTIETENVEFDMSISNPPTVLAPGKYV
                     MLAVTDNGCGMDEKVQAHIFEPFFTTKEKGKGTGLGLATVYGVVKQSGGYIWVYSEPG
                     RGTTFKIYLPRIEEENVPAARDRLPTQKATPRGSEVILLAEDEKGVRELAREYLETSG
                     YTVIVAENGHAAIDLASRHSGPIHLLMTDVVMPGMGGPELAERIKALRPEIKVLYMSG
                     YTDQAIMHQGVLQTGAVLLQKPFTLAALASKLREMLVGETVIH"
     gene            8097..10373
                     /gene="glgB"
                     /locus_tag="VN788_17315"
     CDS             8097..10373
                     /gene="glgB"
                     /locus_tag="VN788_17315"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015246315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HXU22341.1"
                     /translation="MGGKRKLANIEVPAANEDREDLRSLVRAEDSDPFRLLGPHQVSH
                     HGRTALAIRTFRPGADRISVVWGRARTIYPAERIHADGVFEAILPLSAIGLQDKEAAT
                     PSSYRLHFHFTGGSELETYDPYAFPPVLTEYDLYLSGEGTDYQKYQKLGAHLREVAGV
                     QAVHFAVWAPNARRVSVVGDFNSWDGRVNPMRSRAASGIWEIFIPELAEGSLYKFEIF
                     SRVGDRLALKSDPFAFAAELRPNTASVVRNLDTYAWKDSKWLETRASRDWLHSPLSIY
                     EIHAGSWRHKSDEGYRWLTYRELADELIPYVKQMNYTHIEFMPVMEHPFDASWGYQTV
                     GYYAVTSRFGSPSDFMYFVDRCHQENLGVILDWTPAHFPRDAHGLAFFDGTHLYEHED
                     PRKGAHPDWGTLVFNYGRNEVQNFLIANALFWMDKYHIDGLRVDAVASMLYLDYSRQA
                     GEWIPNAFGGRENLEAIALLKRLNEVLHARHPGALTIAEESTSWPAVSRPTYVGGLGF
                     DLKWNMGWMNDVLKYMSLDPIHRQHHHNELTFSMLYAFHENFILPLSHDEVVHGKRAL
                     LEKMPGDDWQKFANLRLLFGFMYAHPGKKLLFMGSELAQRNEFWEGAALDWSLEWSPP
                     HLGIQRLLADLNHLHQREGALHQIDFEWSGFEWLEPNDAAASVLSCLRRGTDSQDIIV
                     AVCNFTPVTRESYRVGVPRPGFYREILNTDSNYYGGSDVGNSGGVQAEPIPWNDRPYS
                     LNLRLPPLAVMYFKPQGS"
     gene            complement(10505..12133)
                     /locus_tag="VN788_17320"
     CDS             complement(10505..12133)
                     /locus_tag="VN788_17320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HXU22342.1"
                     /translation="MPLDAHGKRASQRVVVIGLDGFDLALAKKFFREGSLPNLARLYA
                     HGACYALDHGRDKFSGLAWEHFSSGQAPHDGGRWSAVTFDRRTYAVTQDSTPTTPFLA
                     DLASQTVVFDLPYCNLALAPRVHGITSWGAHDPGVEQASRPAGLHEELLEIFGPYPAA
                     EWIYGFCWPSPTKTRAAGEALVRAIELRTRAALWLLRDRLPEWDLGIVVISESHSAIE
                     PLWHGVDPAHPLHFVESAPFAAAAMHSIYEAIDRSIGDLRQAFPDATLLAVAMHGMGA
                     NDADVAAMALLPEILYRSSFGSPYMRAVRTSGSLPDGTPLLNESDIWDNVMLQAAPKS
                     ARFRHRLARRLSRVTGVKLGVAAPSNLDWMPATRYAPFWHRMPAFALPSYYDGRIRIN
                     VAGRESRGIVRADQYAGVCSKTIEMLNQCTNLLTGEKVISGIYCPKEDPMKVGPTEAD
                     LYIVWESAPLGLRHPRLGSIGPVPYRRTGGHTGEYGFLFVEGFGVVPGDRGIASSFDV
                     VPTVLDLLGEKPRSHISGKSLASSLAAPAQKVQV"
     gene            12409..13308
                     /locus_tag="VN788_17325"
     CDS             12409..13308
                     /locus_tag="VN788_17325"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HXU22343.1"
                     /translation="MTDEKHDSEKRDQPDNDLSRRDFIAMSVAAGVAAAASSASAAAM
                     SVTETDVNVKTPDGTCDAAFIHPATGSHPGVIVWTDVFGLRPSMRDIGKRIAAEGYSV
                     LVPNPFYRVAKAPVFDNVASFNFQNQADRAKLQPLTAALNSPGAVERDAEAYIAFLDA
                     QSQVDTAKKIGTQGYCMGGPLVVRTAAAVPNRVGAGGSFHGGNLVTDKPDSPHLLAPK
                     IKAHMYFAIASNDDKRQPDAKDKLREAFAAAHVPAEIEVYPAKHGWCVPDMPSDASGP
                     IYSKPDADRAWGKLVALYKLALA"
     gene            complement(13645..14070)
                     /locus_tag="VN788_17330"
     CDS             complement(13645..14070)
                     /locus_tag="VN788_17330"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HXU22344.1"
                     /translation="MNAADVPLGKANGKSWLFWSGWVVSLWPVFVVATSAHWKLTHNP
                     AYVREFARIGWPEHALALLACLQLGCIILFLIPQTAVLGAVLLTGYLGGAVAAYTRMG
                     EPYPVLVPLSTSMIAWLGIWLRDGRLRALLPIRRGVAMK"
     gene            14227..14745
                     /locus_tag="VN788_17335"
     CDS             14227..14745
                     /locus_tag="VN788_17335"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU22345.1"
                     /translation="MDSMIDSLFDRYERGSITRRHLVQALAALVLPATLLAQDAAPAP
                     APIVRGLGMNHVGLTVNDIERSFVFYQKLFGVKKGWPATNAGTGIHLDFPTGYISVDS
                     VAEKKGVITHFSVSIDHIDQSSAKILADKINSELPDAKAKAAYQPNDGVSTVNLIDPD
                     GFHVQISPKDGR"
     gene            15027..16457
                     /locus_tag="VN788_17340"
     CDS             15027..16457
                     /locus_tag="VN788_17340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22346.1"
                     /translation="MNKIRLWSFVAIAIIACISAIPTHSARAQQAPAAFTPPPGGNPV
                     LTGQPEPPPAVLDQARFRNPQAPQPGGFTRPRPKPVSEPPQPGVTPLPIDLFTSKNFY
                     LDKASWMDPRYYRCNTPRQMVEAMWESGRIGSNPPTSASWGDCRIDYPRDRIVSPYPY
                     KTAKEHYEALLAQAKAHGGPTVYTKATTPDWDGFYVRDPNGSDSPGFIGPDRRARGFN
                     FLGRGERWQWGGINQVPTLLSLLTPEYQKRYVQMIYHETVDNSKQWSASFCWPEGFTR
                     WWSAPSQASNFELSITPTRVEFLSGIADNFILEFLVGQQQHVQKVPQWYGETIAFWDG
                     DTLVGWTANVQGWDHHTLFEFSNHLEAVETFKPMYDAQHHFVGLDHEVVWYDPDSLVQ
                     PVRLRDRFLRRAVAGDPTARFSFIECLSNIKDVNGRPVQLTKGDPDYIDYYGRPWAEN
                     WEKWFEKGWKKPDDTGVPSDILNLLK"
BASE COUNT         3567 a         4863 c         4473 g         3557 t
ORIGIN      
        1 gtaggcgcac cgcacagcag tatccggcaa ccatccgagc ggagaacggc ggcggacgtt
       61 cgcgcgaagt cttcggaaaa aaaccgggtg tcgtagccca cgatcaccac aggagctttg
      121 gtgtgagcgc gaagatggcc agcaatcgct ctcgatgctc gacgcactcc ggagaaagtg
      181 aagtcttcgg caatgacgcc tcgccagccg gaagtgccga agtggatcgt gtggtttgtc
      241 acgcgcgcgt attgtatcgt gtcaccccag gcgcaactat caggtaatcg cggtaggatg
      301 agccccaaga gtaaagacag catgacaaat aagatcgtcg tcctcgtaac atgcggatca
      361 atcaaggagg ctcaacagat cggccgcgct ctcgtggacc gtaaattggc ggcttgcgct
      421 aacattctcc acgccccggt tcactcgata taccggtgga agggcaaggt ggacgtggct
      481 aaagaaattc tcctgatcct caaaagctcg cgatcgcgtt tcgccgctat agatcgcgcg
      541 attcgaaaca tgcatagcta tgacgtgccc gaaatcattg ccatcccgat cgcggcgggc
      601 tcccggagct atctcgactg gatttctaag tccgtgagcg cggggcaaga gaacgtagcg
      661 cttaaacgcg caacgcatca gacgtccatc ttgagagacg tcggtgatcg tagtaaaaag
      721 agagcgtcat gaaagctaag cgacggaccc gtgccggttt cggatggatc atctatcctg
      781 cgttattggc aatatgcctg ctatgcgtgc cgcaagagtt ggcccaaact gacaattcaa
      841 gccagccccc cgcttcggat caatcgagtg ccaccaagca agaccaatcc agtgcggcag
      901 ataagaagga acagacgcct tccgaatctg ctactgcaag gcttaggatc atcgtcacta
      961 cgggagagga taagcctatt ggcaatgcca gcgtctacgt tcggttcaac acaccgggag
     1021 gctttctgcg tcatgaaaag caggtggaaa tgaatttcaa gacgaatcaa gacggttcgg
     1081 taaaagttcc agacattcct caggggaaaa tactaatcca ggtgatcgcg aagggttggc
     1141 acacatacgg caagtggtac gacgtacaaa aagaggaaga aacaatcgca attaagctag
     1201 ctgcgccacc tcattggtac tgagctgccg tttcgagcct ttccatatca ggaatttccg
     1261 gcatcgcgtt ccattttaga aaactgcgaa cctttccgct gcgaaaagtc ctgccgcggg
     1321 ccgtcgcttg cacaggattt gtgcaaagag cagtaagccc cgtactgtcg cgtgttttcg
     1381 attgttgcgc gaggcttttc cacacggttt tcaacagaac tgttgaaaac tgtcatgcag
     1441 tattcctagt tctcaattct caatcgagaa atggtgcaac gattgcccta aagcgcattc
     1501 ggttctgacg aacggcttcg cgccgtagag tttttgacag ttacactcct caacacgatt
     1561 tgtccgaggc gaacgagctt cgtctcacga gcgtgatacc atcgtcccct gccagtattg
     1621 aataaatgaa agaattaatt cttaatgcgg atgattttgg tttgactcaa ggcatcaacc
     1681 gtggaatcat ctgcgcgcat cgtgacggaa tcctcaccag caccacattg atggctacag
     1741 gtccggcatt tgatcacgca gtcgaacagg cgcgcgcaaa tccaacttta agcgttggat
     1801 gccacttggt cctcacaggc ggttcagccg cagccccccg agagcagatt ccatcactgg
     1861 tggacaagaa tggccgcctg ccccgatctt taaccgtttt ggttgcgagc attaccaccg
     1921 gtaaggtgcc cccgatcgaa atcgaaacgg agttgcgggc ccaaatcgaa aaaattcgcc
     1981 gcgcaggcct cgagcccagc catttggata cccacaaaca cacccacgtt cacccgacga
     2041 tcatgaacgc gctcgggcga gtggctcagc agtccggcat caggcgggtg cgcaacccag
     2101 tggaggatct gcgagactcg tggaggtcca atcggtctga cggcactaac gcggtgagaa
     2161 atttggctgc cgctgcgacg gtaagcgctg tagcttctgg atttaaagcc atttctagca
     2221 agtatggctt gcgttctccc gatcattttt tgggactggc aattaccgga cagttaggtg
     2281 ccgcagcttt gtgccgcttg atcggcgcac tcgatgatgg ccaaaccgaa atcatgctgc
     2341 atcccggaat ttgcgatcag gatctggcac ggaccggctc gcgtcttcag cgtcagcggg
     2401 aagttgagat ggagggtttg ctagcgccag aagtgaagga cgcaatcgtg aagggtggca
     2461 tccgcttgat cagttatcgc gacttgaatt aagctgagcc gaacttcgat ctaacataag
     2521 acaatattcg acgggcttca ggaatcaccg attgctatag tgtctcgctg ttttgagagt
     2581 ttcgaatgac gacgtggtta gccaatcatt gcttggagca tgaaagatga agatcatgga
     2641 acatcgagtg cgtgcccttt tgtacgtggc ggtaatcgtc ggcgtctacg cggtggcgtc
     2701 gaccgtgttt ggccaaactg ccaaaagcca gtacgatggt tcgtaccggg gagcgtacag
     2761 cgggtcgttt gcgagcaact ccgtgatggg atccgtcgaa ttttccgtgg cgaatggtgt
     2821 cattaccgtg acagagcccg gtcggggcgc tggaaccgtc gattcttccg gcgcggcgac
     2881 tttcagcggt tcactcgggg tggcgcgcgt cacctgcaag tttactggtg ctttccggtc
     2941 gtcaacccgc gcacagcaaa gcgttcatgc tacgggttcg tggtcgtgta ccgggtcggg
     3001 gcaaaccgga aagggcacgt ggagtgcaga tcgaccttag cctttcctcg gcccctggcg
     3061 tattctccaa tctaatggtg atgatgccgg ccccgggcaa cagttcattg ctactccagg
     3121 tcggggggcg ttaccttcgc aatccaagag tcataatcgg aagtataacc cgccttctct
     3181 tgcagtgtcc gaatgcgctt cttgatgtca gccaattttt cggacgagag tgccggcgca
     3241 gagcgaatcg tcttctcaac agcttccgcc tgctccgccg cctgagtttc cgcagcttcg
     3301 tataacgccg acagatctcc cacgccatct ccaatcccaa tgtagttttc aatcaggtgc
     3361 ctcgttacgc gctggctgtg aatgagcgac actcggatgt tgccttggcc ggtaaccaca
     3421 tttcccacgc cgaatacgtg ctcgcagcct gaatagcgag gcaagtcttc gccgctaaaa
     3481 gtgtagtatt cacccttcat cgacacgcca ggaatgatct cgggcaccga accgattgac
     3541 gagattacta gcggcgccct caactcttgc tccgagccag gtattggctg ggcacttcgg
     3601 ccttcaattt tggtttccgc caccttcaat ccaaccacgc gaccatcctc tacaatcagg
     3661 gccgttgtca gccggcgatc ctgaacgcga aatagatatt tctctcgcgc tagccgaagc
     3721 atcttttgac gtacttgctc ggtttttgtg atctgttccg cggtggcatt atccggaggc
     3781 tgagcaagag gcatatcttg ttcgcggcgg cgatagatca aaagacaacc cttcacgccg
     3841 agttcctctg gctttatgcc atgagctttg cacgccgctg gtatgccttt ctccaactca
     3901 tgcatattcg ttgcgatacc gcgggcccgc atggcgcgtt catagttttc gagttgcagc
     3961 actttgacca cgtcaatgga cgccaagcct ccacccacga cgatagtttc gtctggagtt
     4021 tcatacagag ggcccgcgta gcccttctca tttttgtggt tgtaccagta aatgaacgga
     4081 ttctgataga tcagcccctt gttgatgaat tgatccgctc ccggcactcc tagatcgcga
     4141 tcgcgccagg caccgttcgc caaaatcacc gctgagaagc cccactcatg gcaaagttct
     4201 tgaaaatcga aatcacgacc gaccttcgtg gacggaatga agtgaacacc cggcttcctc
     4261 aagcgggcat cgatgcgggc gtattcctgt ttgcgctgtt cgacgtgcca gcggggcaag
     4321 ccatcctcga ttttgccgta cggcctcttg ttctgctcga tgacggctac ttgaattccg
     4381 cgctccgcta aaatctccgc tgccacggag ccagagatgg ctgcgcccac aattgcggca
     4441 aaatgttgtg ctttctcggc cattgacgtt ctgcggacct aattttggtg ccgcgaagca
     4501 ccggaattgt acacccgcga gccattggat agtgtcgtgg tgaggaagtt gaagcgagtc
     4561 gaggatcccg ttctttagtg gatgacggtc tcgccgacga gcatttcgcg cagtttcgag
     4621 gccagcgccg ccagcgtgaa aggcttctgc agcaggactg cgccggtttg gaggacacct
     4681 tgatgcatga tcgcctggtc cgtataaccc gacatataga gaaccttgat ttcgggacga
     4741 agagctttga tccgctccgc gagttcgggc cctcccattc ccggcatcac cacgtccgtc
     4801 atgagcaagt gaatcggtcc ggaatgccgg ctggccaagt caatcgctgc atggccgttc
     4861 tccgccacaa tcacggtata accgctggtc tctagatatt cgcgtgcaag ttctcgaacg
     4921 cccttctcgt cttccgccag cagaatcacc tctgagccgc gcggcgtggc cttttgggtc
     4981 gggagccggt ctctagcggc agggacgttc tcctcctcga tacgcggcag ataaattttg
     5041 aacgtagttc cacggccggg ctcgctgtaa acccagatgt acccgccgct ctgcttcacc
     5101 acgccgtaga ctgtcgccag tcccaggccg gtgcctttgc ctttttcctt ggtagtgaag
     5161 aacggttcga aaatgtgcgc ttgaaccttt tcgtccatgc cgcacccgtt atccgtcacg
     5221 gcaagcatca cgtattttcc gggcgcgaga acggtggggg gattcgaaat cgacatatcg
     5281 aattctacgt tctccgtttc aatcgtcagc cttccacccg tgggcattgc gtcgcgggcg
     5341 ttcaccgcca aattcatgat gacctgttcg atctggctgc ggtcggcttt tgtcagccac
     5401 agatccgccg tggaagacgt gcgcagttcg atgtcctccc ctatcacgcg ccgcaatatc
     5461 ttttccaggt cggtgacgac agcgtggacg ttgagcactt tcggagcgag tatttgctta
     5521 cggctgaaag ctagcaattg ctgcgtcagc gctgccgccc gttgcgaggc ggcccggatc
     5581 tgttccgcgt ttcgacgcag agcctccccc tccgggagcc gctcggccag caaatcggca
     5641 tagccctgaa ttaccatcag caagttattg aagtcgtgcg cgattccgcc cgccaaccgg
     5701 cctaccgcct ccatcttttg cgcctgccgg aattgttcct cgagcttctt gcggtcggtt
     5761 acatccaaag ccatacagat cgcgccgtga acggcgccat cgccatctcg caatggttcc
     5821 acgtgacacg cgtacgaacc atctttccac tccacgggga acgtcactgc ttctccgacc
     5881 acggcccggc gatgcgccgc aatgggcaaa aaagtcgggt cggacgtctc gaaatactca
     5941 gttaacgaca tgcccacgat ttggtcgggc ttcagtccca gccgtattag ccctgctcca
     6001 acagccgacg taaaccggag attgctgtcg actgtccaca ataccgccgg caactgctcg
     6061 atcagaacgc gcagcctcgc ctcgctctgc cgaactcgtt cctctccccg tttgcgctcg
     6121 atcgcaagcg cgatctgatc cccgaccgac gatagaaatt cgaggtcccg ttccgtgtac
     6181 gcattctcaa tggagtagtt ctgaaccacc agcaccccga tggtttccgt tggggtacga
     6241 agaggaaccc ccagccagga tgcggacggc gtgcctacta attcaacttc tcccctgcgc
     6301 gcaaggtctc ggaagacttc tcgcgggatc agcatcccgc gcgcattacg aaacacgtac
     6361 gctgcgcaac ttcggtccat cgtgtcgggc ggaggcggag gatcgaactg atctataaaa
     6421 aaactgtagt ggaacatccc cgtagaacgt tcgtgaagtg ctacgaagca gttctccgcg
     6481 tccagtacct gcttgagcgc cgcgtgaatt aagcgaagca agtcgtcaag attgtcggtg
     6541 gcgctcacgg cgtggacgat ttccgtgttc acctgccgtt ccagctgcga gcgcttgcgc
     6601 gcggtaatat cttccgccgt gccgtcgtgg cttacgatct tgccggaggc gtcgcgtacc
     6661 gcccggacgt tcagcgaaat ccatattttg cttccatctt ttcgcaacat cgcacattcg
     6721 aattcttcga gaacgccccg ctgctccaaa cgccgcttca cttcttcccg gacgctcgga
     6781 tcggtgtaga tctgccgccc gatatcatga atctcactga tcatctctgc cggcgacgca
     6841 taacccgcga ttcgtgccat ggcgggattt acgaccagaa agcggccagc cggcgttgat
     6901 tcgaaaaagc cttcgactgc gttgtcaaaa atgcgccggt gcttttcctc cgcccggtga
     6961 agggcttctt cggcctgttt cttctccgta atgtcttcgt acataccgaa cgtgccggtt
     7021 cgttttccat caacgatgag cggcacgcct atcacctgca cgtccacgca cgagccgtcc
     7081 ttgcgctttc gctttgtcgt tgcgcgcacg atatcgccgg aaaccgctcg ccccataagt
     7141 ccgagcattt cacgatgcag gtcctcggga gcgatcagcg agtccagttc cgcgcctcga
     7201 atttcggcaa gcgaatagcc gaatagccgc tcgaaggcgg ggttgcacat ttctacgcgg
     7261 cgctccatat tcacggccac aatagcggtc ggattgtttt cagtcagggc ctgggcatag
     7321 gctgtccgtt ccgcagtttt ttcctccatc gcgcgccgta cgctgatatc ctgtgccacc
     7381 accagaaccc cgtcccgtcc cgcgaaggtg gttccctggg ccgtcacctc aacatcaaag
     7441 atctctccgt ttctccggcg atgccgccac tggccgagaa acgcgagcga tggcccattg
     7501 gttcgggcga tgtccaggag gcgtgggatg tcctcggcgg gccgtatatc cgtgatcttc
     7561 atcttcagga attcttgccg cgtgtagcca tactgcttct cggccgctga attgacttcc
     7621 aggaattcca gcgtttcacg gtcgcacacg tacattgcaa gggggttggt ggaaaacagc
     7681 agctcgaaat tcgattcgca atcgcgcaag gaccgatgcc cagaagtctt gcttggcccg
     7741 gggtccgatt cggagcgtag gctgtcggga cgcaccgtac tcgtcttaat cgccataacc
     7801 ggactgtccg ctcctatgcg agatgggcgc agctctccga gagcgagcct cgttcttttt
     7861 atagtcgccc gctcagtggt ccacaatggt acgtcagtac caggatcgca gatatcacgt
     7921 tgtgtttcca attagatacc ggccctaacg cgccggcaca atctgcagta cagcgctgcc
     7981 ggcacgtcct cctcaacacc tttaatacct ttcgggcccc ggctaggcac cggtttggca
     8041 tcctgctcac gcttcggtag aatgacaaaa gagcctcaaa aagcaggctc cagaccatgg
     8101 gcggtaaacg aaaactcgcg aatatcgagg tcccggcggc taatgaggac agagaagatc
     8161 tacgcagcct cgtgcgcgcc gaggattccg atccctttcg ccttctgggc cctcatcaag
     8221 ttagccatca cgggcgcacc gcactcgcaa tccggacctt tcgccccggc gcagatcgca
     8281 taagtgttgt ttggggcaga gctcggacca tttatcccgc tgagcgcatc catgcggacg
     8341 gcgtgtttga agcgattctt ccgcttagcg caatcggctt gcaggacaaa gaagccgcca
     8401 cgccctcctc ctatcgtttg cattttcatt tcaccggcgg aagcgagttg gaaacctacg
     8461 acccatacgc tttccctcct gttctcaccg aatacgatct ctatctttcc ggcgagggga
     8521 cggattacca gaaatatcaa aagttgggcg cacacttgcg ggaagtggcg ggagtacaag
     8581 cagttcattt cgccgtgtgg gccccgaacg cccggcgagt cagcgtagtt ggcgacttca
     8641 actcgtggga cggacgcgtg aatcccatgc gcagccgcgc cgcaagcggc atctgggaaa
     8701 tttttatacc cgaactcgcg gaaggctcgt tgtacaaatt cgaaattttc tcacgcgtgg
     8761 gagatcgcct cgcgctcaag tccgatcctt tcgcgttcgc cgccgagctt cggcccaata
     8821 ccgcttctgt cgtccgtaat ctggacacgt acgcctggaa agattcgaag tggctcgaga
     8881 cgcgtgcctc tcgcgactgg ctgcattccc cgctctccat ttatgaaatc cacgccggtt
     8941 cctggcggca taagagcgac gaaggctacc gctggctcac ttaccgcgag ctggccgatg
     9001 aactcatccc ctatgtcaag caaatgaact acacgcacat cgaattcatg ccggtcatgg
     9061 aacatccctt cgacgcttcc tggggctatc agactgtggg ctactacgca gtgaccagtc
     9121 gcttcggctc gccctccgat ttcatgtatt tcgttgatcg ctgtcatcaa gaaaatctgg
     9181 gcgtcatact tgactggacg cccgcgcact tcccgcgcga tgctcacggc ctggcctttt
     9241 tcgatggcac tcacctctac gaacacgagg acccgcgaaa aggcgctcat cccgattggg
     9301 gaaccctcgt cttcaattac ggccgcaatg aagtgcagaa tttcctcatc gccaatgctc
     9361 tcttctggat ggacaaatac cacatcgatg ggcttcgcgt ggacgcggtc gcttcgatgc
     9421 tctatctgga ttactcgcgg caggccggcg agtggattcc caatgcgttt ggcggacgtg
     9481 aaaatctaga agcgattgcg ctcctaaagc gtctgaatga agtattgcat gcgcgccatc
     9541 cgggtgcctt gaccatcgcc gaggaatcca cttcttggcc cgcagtatcg cgccccacct
     9601 acgtaggagg cttggggttc gatctgaagt ggaacatggg ttggatgaac gacgtgctta
     9661 aatacatgtc cctcgatcct atccaccggc agcatcacca caacgaactc accttctcca
     9721 tgttgtacgc cttccacgaa aatttcatct tgccgctttc gcatgacgaa gttgttcacg
     9781 gcaagcgagc actgctcgaa aaaatgcccg gtgacgactg gcagaaattc gcgaatttgc
     9841 gcctcctctt tggcttcatg tacgcgcatc ctggaaagaa gctgctcttc atgggaagcg
     9901 agctggcgca gcggaatgaa ttttgggaag gcgccgcact ggattggagc ctcgaatggt
     9961 cacccccgca cctcggcatc cagcgactcc tcgccgacct caaccatctt caccaacgcg
    10021 aaggcgcatt gcatcagatt gacttcgagt ggtccggatt cgaatggctt gagccgaatg
    10081 acgccgccgc aagcgttctt tcctgcttac ggcgcggaac ggattcccaa gacattatcg
    10141 tcgccgtttg caattttacc ccggtgacgc gcgaaagcta tcgtgtgggc gttccgcgtc
    10201 cgggctttta ccgcgaaatc ctcaatactg attcaaatta ttatggcggt tcagatgtgg
    10261 gtaactcggg cggcgtacag gccgaaccaa ttccatggaa cgaccgccct tactcgctga
    10321 atttacgcct gcccccgctc gccgttatgt acttcaagcc tcaagggtct taacagctgg
    10381 catttacgcg cccgtcaccg gttcgaactt cgcaggcacc atttccacca atgttcatgc
    10441 ggccttgcgc gtgttggctc gcacagaagc gcaaagaagc gcaggctgcc tccaatcggg
    10501 actgtcaaac ctgaactttt tgcgctggag cggcaaggct gcttgctagc gacttaccgc
    10561 tgatgtggga tcttggtttt tcgccgagca ggtcgaggac ggtcgggacg acatcgaaag
    10621 aactcgcgat ccctcggtca cccggtacca caccaaaacc ttcgacgaag aggaaaccat
    10681 attcgcccgt gtggcctccg gtgcgacgat agggcactgg accgatagac ccgagccgcg
    10741 gatggcgcaa gccaagcggc gcggattccc acacaatgta taggtccgct tcagtcggtc
    10801 ccactttcat gggatcctct ttagggcaat agataccaga gatgactttt tctcccgtca
    10861 aaagatttgt gcactgattc aacatttcta tcgtcttgct gcagaccccg gcatactgat
    10921 cggctcgaac gattcccctg gactcgcgcc cggctacgtt gattcgaata cgcccgtcgt
    10981 aataggaagg gagggcaaac gcgggcatgc ggtgccaaaa gggagcgtac cgagtggccg
    11041 gcatccaatc gagattgctt ggggcagcaa cgccaagttt gacccccgtg acgcgcgata
    11101 ggcgacgggc aaggcggtgg cgaaatctgg ctgacttcgg cgcggcttgc agcatcacgt
    11161 tgtcccagat gtcactttcg ttgaggagtg gcgtgccatc aggcagtgag ccggatgtgc
    11221 gaacggcccg catgtacggc gagccgaaag aagagcggta gaggatttcg ggtagtaagg
    11281 ccatcgccgc cacatcggca tcgttcgctc ccatgccatg catcgcaaca gcgagaagcg
    11341 tcgcatcggg gaaggcttgg cgcaggtcgc cgatggaacg gtcgatggct tcatagatgc
    11401 tatgcatcgc agccgcggcg aaaggagccg attcaacaaa gtgtagaggg tgagccggat
    11461 cgacgccatg ccatagaggt tcgatcgcag aatggctctc ggagattacg acgatgccga
    11521 gatcccattc gggaaggcga tcgcggagga gccaaagggc agcgcgcgtg cgaagttcga
    11581 tagccctcac cagcgcttcg cctgcggcgc gtgtcttggt tggcgagggc cagcagaaac
    11641 cgtagatcca ttccgccgcg gggtatggcc cgaagatctc gagcaattcc tcgtgaagcc
    11701 cagcgggacg cgatgcttgt tccacacccg gatcatgagc gccccagcta gtaatcccat
    11761 gtacgcgcgg tgccaaggcg agattgcagt aaggaagatc gaagacaacc gtttgactcg
    11821 caaggtccgc gagaaaaggc gtggtcggag tactgtcctg cgtgaccgcg taggtcctgc
    11881 ggtcaaatgt gaccgccgac cagcggccgc cgtcatgcgg cgcttgtccg gaactgaaat
    11941 gttcccaggc taagccagag aatttatccc gaccatggtc gagggcgtag cacgcgccat
    12001 gagcatacaa acgagccaag ttgggaagcg agccctcgcg gaaaaacttc ttggctaaag
    12061 ccagatcgaa cccatcgagc ccgataacta ccacgcgctg cgaagcccgt ttgccatggg
    12121 catctaaagg cataattcct cgatggacat ctgcgtttcg atcccgggtt gcagaggtac
    12181 ccgcatcagt aagggtccct aggtgcactg acggcgggtt tcgatgggtt ggagccgcgg
    12241 ggaatggcac cttagcccgg cgggagagta gcacaaagga gcgagacgcc caagagcggg
    12301 tttatggatg cgatccgagc ttgacactgg aaggcggctc acatatggtc cgcgccagga
    12361 cgagtggaag cataaattct ggactcgtcc acaagaagga gtaagccgat gactgacgaa
    12421 aaacacgatt ccgaaaagcg ggatcagccg gacaatgatc tctcgcgacg cgacttcata
    12481 gcgatgtcgg tggcagcggg cgtggcggcc gcagcaagtt cagcatcagc ggctgcaatg
    12541 tcagttacgg agaccgacgt caatgtcaaa acccctgacg gcacctgcga tgccgcattc
    12601 attcatcccg cgacgggatc tcatccgggc gtaattgttt ggaccgacgt gttcggattg
    12661 cgcccgtcga tgcgtgatat cggtaagcgc attgctgccg aaggctactc ggtccttgtg
    12721 ccgaatccat tctatcgggt ggccaaggcc cccgtgttcg acaatgtcgc gtcgttcaac
    12781 ttccaaaatc aggcggacag ggcgaagctg cagccgctga ctgccgctct gaactcccca
    12841 ggtgcggtag aacgggatgc ggaggcgtac atcgcattcc tggatgctca atcgcaagtg
    12901 gacacagcga aaaagatcgg cacccaaggc tattgcatgg gtggaccgct ggttgtgagg
    12961 accgctgccg ctgtgccgaa tcgtgtgggc gcaggtggct cgttccatgg gggaaatttg
    13021 gttacggaca agcctgacag tccgcacctg ctcgccccga agatcaaagc gcacatgtat
    13081 ttcgccatcg catcgaatga cgataagcgt caaccggatg cgaaagacaa gctgagagag
    13141 gctttcgcgg cggcgcacgt tccagctgaa atcgaagtct atccggcgaa gcacggctgg
    13201 tgcgtgccgg acatgccgtc agacgccagc ggaccgatct acagcaagcc tgatgcggac
    13261 cgtgcgtggg gcaagctcgt ggccctctac aaacttgcct tggcttaacg gcttcttttc
    13321 ctaatttgcg cccgctctgt ccatgaactt ggacgcattg gcgagttacc ggacaatttc
    13381 agtagcagtc cggaagatgt cccattgacc ccttacctcg tttgcaccga tccctacgtg
    13441 ggatcgcgtc cgcgaggatt accttagccg gtgccctaca aagtcgggtt ttctttacac
    13501 caaccgtgga cgacttttct ttacaccaac aaccacttgc actccccacg tgccttgggc
    13561 taaaaatatt aatgtatcct cgtagtgtgc ccggacgcga agaaccaacc cgttcgccag
    13621 tcttttccgt cgatgttcca gattctattt catcgctact ccgcgccgga tcgggagcag
    13681 cgctcgaagg cgcccgtccc gcagccaaat ccccaaccac gcaatcatgc tggtcgatag
    13741 cgggacgagc accggatatg gctctcccat acgcgtatag gcggcgacag ctccgccaag
    13801 ataaccggta agaagcactg ctcccagaac ggcggtctgc ggaatcagga agagtatgat
    13861 gcagcccagt tgcaggcagg caaggagcgc cagggcatgc tccggccagc cgattcttgc
    13921 gaactccctg acataagcag gattatgagt cagcttccaa tgcgcgctcg tcgccacaac
    13981 gaaaacaggc cagagcgaaa ctacccagcc actccaaaat agccaactct tcccgttcgc
    14041 tttgcccaat ggaacatccg ctgcattcat gtcttcctct ccttctgctg cccttcagtg
    14101 cgagtacggc tggccgggct tcatgcactt gggatgcgct cccccgccgc gcaacatggc
    14161 agcgcatgat accatcgccg acgcttcacc cgatgcgact gaaacgcccc gaaggaggaa
    14221 acaataatgg attccatgat tgattcgcta ttcgatcggt atgaacgcgg cagcatcaca
    14281 cggcgtcatc ttgttcaagc gctggccgca ttggtcttgc cggcaacact cctggcgcag
    14341 gatgccgctc cggcgcccgc tccgattgtt cgcggccttg gcatgaacca cgtcggtctc
    14401 acggtcaacg atatcgagcg ttcctttgtg ttttaccaaa aattattcgg agtcaaaaaa
    14461 ggctggccgg caaccaacgc cggaacgggc attcatctgg attttccgac cggctatatc
    14521 agcgtcgact cagtcgcaga aaaaaaaggc gtgatcacgc atttctccgt gtccatagac
    14581 cacatcgatc aatcttctgc aaaaatcctg gcggataaaa tcaatagcga gctgccggat
    14641 gccaaagcga aagctgcgta tcagccaaat gatggagtct ccacggtcaa tctcatcgat
    14701 cccgatggct ttcacgtgca aatctcgccc aaagatgggc gctgaacgaa ccctaggaat
    14761 tccatctggc aagctaacgg tgaatccgca tcgaatttta tccgcagctg ctgggattag
    14821 aaacttgaca gtcgggtaca aaatggttgc gaatcaaaat gctcacttcc ttccgtagga
    14881 aggtgaaggg cacgagcgcg aaattagcta ctctgagaac gatgaatttc accgcatgtt
    14941 accgtaggcc gcagttatga gatgcacgtg gcgccaatgc ttgtgctcca gacgaaatca
    15001 aagaatgaag agaggtaaca aaagtcatga ataagatacg attatggagc tttgtcgcca
    15061 tcgctatcat tgcctgtatc tctgcgatcc caacccactc cgcgcgcgca cagcaggcac
    15121 cagccgcgtt cactcccccg ccgggcggca atcccgtcct cacgggccag ccagagccgc
    15181 cgccagccgt tctcgaccaa gcccgcttcc gcaatccaca ggctccgcaa cctgggggat
    15241 tcaccaggcc ccggcccaag ccggtctcgg agccgccgca accgggcgtt actccacttc
    15301 cgatcgatct cttcacgtcc aaaaacttct atctcgacaa agcctcgtgg atggacccgc
    15361 gctactaccg ctgcaacacg ccgcgccaga tggttgaggc catgtgggag agcggacgca
    15421 tcggctccaa tcccccaact tcagcctcct ggggtgattg ccgcatcgac tacccgcgtg
    15481 atcgcatcgt cagcccgtat ccttacaaga ctgccaaaga gcactacgag gctctcctcg
    15541 cccaagccaa ggctcatggc ggccccacgg tctacaccaa agccactaca cccgattggg
    15601 acggcttcta cgtccgcgat cccaacggaa gcgactcacc cggcttcatt ggccccgacc
    15661 gccgtgctcg cggattcaat ttcctcggac gcggcgaacg ctggcaatgg ggcggcatca
    15721 accaggtacc gacgctgctc tcgcttctaa ctcccgaata tcagaaacgc tatgtccaga
    15781 tgatctacca cgagacggtg gacaactcga agcagtggag cgcctctttc tgctggcccg
    15841 aagggttcac ccggtggtgg tctgctccct cccaggcgtc caacttcgaa ctctccatca
    15901 caccgacccg cgtcgagttc ctctctggta ttgccgacaa tttcattctc gagtttctgg
    15961 tcggccagca gcagcacgtg cagaaggttc cgcaatggta cggtgagacg atcgctttct
    16021 gggatggcga taccctggtc ggctggaccg ccaacgtaca gggatgggat caccacaccc
    16081 tgttcgagtt ctcaaaccat ctggaagcgg tcgaaacctt caagcccatg tacgacgcgc
    16141 agcaccactt cgtcggcctt gaccacgagg tggtctggta tgacccggat tcgctggttc
    16201 agcccgtgcg cctccgcgac cgcttccttc gtcgcgctgt cgccggcgat cccaccgccc
    16261 gattttcctt catcgagtgc ctgtcgaaca tcaaggacgt caacggacgg ccggttcagc
    16321 tcaccaaagg cgatccggat tacattgact attacggacg accgtgggcg gagaattggg
    16381 agaaatggtt cgaaaaaggc tggaaaaagc ccgatgatac cggcgtgccg agtgacatac
    16441 tcaacctcct aaaatagaag
//
LOCUS       DATNDT010000530         3700 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2200297, whole genome shotgun sequence.
ACCESSION   DATNDT010000530 DATNDT010000000
VERSION     DATNDT010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2200297"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            370..1224
                     /gene="mutM"
                     /locus_tag="VN788_17345"
     CDS             370..1224
                     /gene="mutM"
                     /locus_tag="VN788_17345"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HXU22347.1"
                     /translation="MPELPEVETVARGLRAVLPGRRILSVRLGKTDFIEDPAALEQCL
                     PGCRITAVRRHGKFLVLDLDPGRTDDGRPSLLIHLGMTGQLVVRPSEAPAMPHTHVFL
                     ALDDGRELRYTDIRRFGRMRIVQDGEHESTLGELGLDPLEATEKEFSAKLRGRRARIK
                     ALLLDQHVLRGMGNIYTDESLWRAHIHPARLGSTLKLAELRNLYRAVRAVLLEAIRLR
                     GSSVSDYVDSDGQRGEFQLRHRVYQRTGKKCFRCGTKIRRAIVAGRGTHFCPRCQCPP
                     RKSKNLSS"
     gene            1339..1605
                     /locus_tag="VN788_17350"
     CDS             1339..1605
                     /locus_tag="VN788_17350"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HXU22348.1"
                     /translation="MEIEFDPAKNRKNIQERGLPFALVAEFDWTTALIAASDPRGESR
                     FMAIGYIGSRLHALVFQANRAALRVISLRRANKKEVKRYEKARS"
     gene            1586..1855
                     /locus_tag="VN788_17355"
     CDS             1586..1855
                     /locus_tag="VN788_17355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014159140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HXU22349.1"
                     /translation="MKKPDPEMIDKQNPEWTDAMFKRAKPASEVFPELVAHSEKRKRG
                     QRGPQKKPRKVLISLRLKRSTIEAYRASGKGYQRRMAEVLDKHAK"
     gene            1883..3343
                     /locus_tag="VN788_17360"
     CDS             1883..3343
                     /locus_tag="VN788_17360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HXU22350.1"
                     /translation="MPDAPTTAALLFAQLAAPLIPRAELRWEFANIAAAIALLSIALA
                     AIVFFLFRRSTRDLTLIFFGLFCTLYAIRLLANVPSFRSLFNGSQDFWGYVDWIITCT
                     IILPFGLFLYQIVGESLKRFIRWLIAVQAVFAVYGILAAAFGVGLRKLFVANNVLVLA
                     TFVALALFLIVTRRPGSLKGLTREIRVFIAGFVVWIVFIVQENLVGLKVLPGHNVEFV
                     GFLIFVACLGYVSARRIFANEERLLAINKELEIARQIQSSILPQSIPVFAGLEIAARY
                     VPMSAVAGDFYDFLIVDDKRIGILVADVTGHGVPAALIASMLKVAFAGQAAHAHDPAR
                     VLTGLNAALCGKFEEHFVTAAYLFLDLEKNLSRYSAAAHPPLLVASRGAGRVREIEQN
                     GLMLGMFPEADYSSVEICVVPGDRCLLCTDGVFEAKNSAQEEFGKSRCKKVLETLLDI
                     PASRFADALLNSIADFSGHNSNRAQDDDITLLVLDL"
     gene            complement(3473..>3700)
                     /locus_tag="VN788_17365"
     CDS             complement(3473..>3700)
                     /locus_tag="VN788_17365"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HXU22351.1"
                     /translation="GGSRAMVTPHDGDGELHIAFAISTAQLDDWERWLGEKGIEVEEK
                     HAWEGGGLSLYFRDPDRHLLEVVTPGTWSIY"
BASE COUNT          745 a         1181 c          916 g          858 t
ORIGIN      
        1 gattacaccg gctttggctt ccatcaacct gacctccctc tgaaatcccg tggccctgct
       61 ctacagtgtt ctctgtattt tcaacactaa aatgctagca cacaccccca gttaggtacc
      121 acaaactttt ttgacaaaac cgtgtggctc cgtgcctcca cgtctttcat tttgcttcag
      181 ctattcccgt atgctgcgcc cggcaaacat gtttggcttc ttgctcgcga tccgcttcga
      241 tgctctcgta gcgccgccat cctgacggcg aatttccgcg cgcctgccga aaccaaacca
      301 gccggccgga aaccgcttgg attgaaaatg ttgccgccat ccacactatg ctaacgttga
      361 atcgcaaaaa tgcccgaact ccccgaggta gaaaccgtcg cgcgcggcct gcgagctgtg
      421 ctgcccggac gacgaattct cagcgttcgc ctcggcaaaa ccgatttcat cgaagatccc
      481 gccgccctcg agcaatgtct ccctggctgt cgcatcaccg ccgttcggcg acacggaaaa
      541 tttctcgtgc tggacctcga tcccgggcgt accgatgacg gccgcccatc tctactgatt
      601 catttgggaa tgacgggcca gcttgtcgtt cgcccatcgg aagcgccggc aatgccccac
      661 actcacgtct ttctcgcgct cgacgacggc cgcgagctgc gctacaccga catccgtcgc
      721 ttcggacgca tgcgaatcgt ccaagatggc gagcacgaat ccactttggg cgaactcggc
      781 ctcgatcccc tcgaagccac cgaaaaagaa tttagcgcca aattgcgagg ccgccgcgcc
      841 cgcataaaag ctctcttgct cgaccagcac gtcctccgag gcatgggaaa catctacaca
      901 gacgaaagtc tctggcgcgc ccacattcat cccgcgcgtc tggggtccac gctcaagctc
      961 gctgagcttc gcaatcttta ccgcgccgtt cgcgctgtgc tactcgaagc aatccgcctg
     1021 cgcggctcgt ccgtttccga ttacgtggat tccgacggcc agcgcggcga atttcagctt
     1081 cgccatcgcg tctaccaacg caccggcaag aaatgttttc gctgcggaac aaaaatacgc
     1141 cgcgccatcg tagccggccg cggcactcac ttctgccccc gctgccaatg ccctcctcgc
     1201 aagtcgaaga atctctcttc gtaaggcccg cgccttctct gccgtcgtag ggccgcggca
     1261 tgccgtgccc ggcaaactta cttggctcca ttatggatat gtttgacaag tctttttaat
     1321 tatatataca attaaatagt ggagattgag ttcgacccag cgaaaaatag gaagaacatt
     1381 caggaacgag gacttccatt tgcattagtc gccgaattcg attggaccac agctctgatc
     1441 gcggcatccg atccccgggg ggaatctcgc ttcatggcta tcggttacat cggcagtcgt
     1501 cttcacgctt tggtcttcca ggcaaaccgc gcggcgctcc gggtcataag ccttcgccgc
     1561 gccaacaaga aggaggtaaa acgttatgaa aaagcccgat cctgagatga tcgacaaaca
     1621 gaatccggaa tggaccgatg ccatgtttaa aagagcgaaa ccggcatccg aagtctttcc
     1681 cgaactggtt gcgcattcgg aaaaacgcaa gcgcgggcag cgtgggccgc agaagaaacc
     1741 gcgcaaggtc ctgatctctt tgcggcttaa gcgcagcacg attgaagcct atagggcctc
     1801 tggcaagggt tatcagcgcc gcatggccga ggttctggat aagcacgcga agtaaatcga
     1861 ctcggctaca attccatccc acatgccgga cgctccgacg acagccgcac ttctcttcgc
     1921 tcaactcgcg gctcctctca tcccgcgcgc ggagctccgt tgggaattcg ccaacatcgc
     1981 ggctgctatc gcccttctgt caatcgctct cgccgccatc gtctttttcc tttttcgccg
     2041 cagcacacgc gacctcactc tgatcttctt cggcctgttt tgcactctct acgcaatcag
     2101 gttgttggcc aatgttccgt cttttcgctc gctgttcaac ggatcccagg atttttgggg
     2161 ctatgtcgat tggatcatca cttgcaccat tattcttccc ttcgggcttt tcctttatca
     2221 gatcgtcggc gaaagcctca aacgattcat tcgctggctc atcgctgtgc aagcagtttt
     2281 cgcggtttac ggaattctgg ccgccgcgtt cggggtcggc ctgcgcaagc tcttcgtcgc
     2341 aaataacgta ttggtgctcg ccacgttcgt ggcccttgca ttgtttttga tcgtcacgcg
     2401 gcgtcctggc tcactcaagg gcttaacccg cgaaattcgc gtctttattg ccggtttcgt
     2461 ggtttggatt gtcttcatcg tccaggaaaa tctcgtggga cttaaggttc tgcccggcca
     2521 caacgtcgag ttcgtgggtt tcctgatttt cgtcgcgtgc ctcggatacg tttccgcgcg
     2581 ccgcatcttt gcaaacgaag aacgcctcct cgcaatcaac aaagagctgg aaattgcccg
     2641 ccagattcag tcctccattc ttccccaaag cattcccgtc ttcgcaggac tcgaaatcgc
     2701 cgcacgctac gtccccatga gcgccgtcgc cggtgatttc tacgatttcc tcattgtgga
     2761 cgacaaacgt ataggcattc tcgtagccga cgtcaccgga cacggcgtgc ccgccgcgct
     2821 gatcgcctcg atgttgaagg tcgcatttgc tggacaagcc gcgcacgctc acgatccggc
     2881 gcgcgttctc accggcttga acgcggctct gtgcggcaaa tttgaagagc acttcgtcac
     2941 cgccgcctat ttatttctcg atttggaaaa aaatctctcg cgctattccg ccgcggcgca
     3001 cccaccgctc ctggtcgcct cgcgtggcgc gggcagagtc cgggaaattg aacagaacgg
     3061 cctgatgttg gggatgtttc ctgaagcaga ctattcatcc gtggaaatct gcgtagttcc
     3121 tggagatcgc tgcctacttt gcacagacgg agttttcgag gccaagaatt ctgcgcaaga
     3181 ggaattcggc aagtcgcgct gcaaaaaagt tctcgaaacg ctgctcgata ttcccgcctc
     3241 ccgtttcgcc gacgcccttc tcaacagcat cgccgatttc tccggccaca attccaatcg
     3301 cgcgcaggac gacgacatca ccctcctcgt cctcgatttg taaaccggac tgctgccagc
     3361 gtgtgacgcg agaatctttt ttaggggcgt agcactgcta cgcccggcaa acttgcttgg
     3421 cgcgggcgcg ccatcggcct cgtatgtagg gcgagcgcac agtgcagata cttcaataaa
     3481 tcgaccacgt ccccggcgtg acgacttcga gcaaatgccg atccggatct cgaaagtaga
     3541 ggctcagtcc gcctccttcc cacgcatgtt tctcttccac ctcaattcct ttctcgccga
     3601 gccaccgctc ccaatcgtcc aattgagcag ttgaaatcgc gaacgcgatg tgcaattcgc
     3661 cgtccccgtc gtgcggtgta accatcgcgc gcgaacctcc
//
LOCUS       DATNDT010000531         4092 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3319985, whole genome shotgun sequence.
ACCESSION   DATNDT010000531 DATNDT010000000
VERSION     DATNDT010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3319985"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            14..1366
                     /locus_tag="VN788_17370"
     CDS             14..1366
                     /locus_tag="VN788_17370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HXU22352.1"
                     /translation="MATKTLIGPSIKTALPGPNAARVLAGDEKYISPSYTRSYPLVAK
                     SGRGLTVTDVDGNEFFDFSAGIAVTSTGHCHPEVVAAIQKQAAELIHMSGTDFYYQSM
                     VTFAERLSKIAPMAGPHKIYYGNSGAEAIECALKLARYHTKRQNIIAFYGAFHGRTMG
                     ALSLTASKPQQRRRFAPLVPGVTHVPYPNIYRDCKNPDDAENYALICARFIEEKLFKT
                     TLPPEEVAAIFVEPIQGEGGYLVAPTIFMQELRRICDRHGILLVADEVQSGAGRTGKW
                     WAVQHTGVEPDIVCIAKGIASGMPLGITMSRAEVMDWVPGSHASTFGGNPVCLAAALA
                     TLDVIEHERLLENSTEVGNYMLKRLADWPAKHKLVGDVRGRGLMIGVEIVTDKKTKAA
                     ATKERDRILELAFERGVLFLGCGPSTVRIAPPLIVTKEQADVAMDALEESIAIVARSA
                     "
     gene            1741..2190
                     /locus_tag="VN788_17375"
     CDS             1741..2190
                     /locus_tag="VN788_17375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HXU22353.1"
                     /translation="MPSGRLTHATIRALDWLAEKTLPPQNIPPHQRTGRQGEEAAYFY
                     LRRLGYIMVARNFRSPHRRGEIDLIAWEKDVLCFIEVKTRTTHDVKPAEAAVDADKQR
                     ELNYMAREYLRHLPPSCQWRFDVLSVYYEQPAKSPRFELFRNAIPLS"
     gene            2257..3297
                     /gene="galT"
                     /locus_tag="VN788_17380"
     CDS             2257..3297
                     /gene="galT"
                     /locus_tag="VN788_17380"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014962084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HXU22354.1"
                     /translation="MPELRKDPIVGRWVIISTDRAKRPNDFSREVIQLKGGFCPFCYG
                     NEAKTPPEIQAYRPNHNGGPPPQRDTPGWTVRVVPNKFPALGIEGSLNRQAEGMFDKM
                     NGIGAHEVIIETPDHATTLAMLAPKRIEDILWTFRDRILDLKKDRRFKYILTFKNHGE
                     AAGASLEHSHSQLIALPILPIYVAEELEGAKQYFDYKERCVFCDIIHQETEAGVRVVA
                     ENEDFLTIAPFAPRFPFETWVLPKQHESAFEHSSSHMFENLAKALKLLLNKADRVLDN
                     PPYNLVIHSSPVQEPNNDYYHWHIEFMPKLTKTAGFEWGTGFYINPTPPEEAAKFLRE
                     ANVEEPITATVR"
     gene            3424..>4092
                     /locus_tag="VN788_17385"
     CDS             3424..>4092
                     /locus_tag="VN788_17385"
                     /inference="COORDINATES: protein motif:HMM:NF014851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta galactosidase jelly roll domain-containing
                     protein"
                     /protein_id="HXU22355.1"
                     /translation="MKRLLSFFILLSSLCFAAPAPSNVIANIPGRTAVSLNGAWNIII
                     DPYESGLGSRYYLNAKPKDKSDLVEYDFDKSPTLKVPGDWNTQRDELLFYEGPIWYRR
                     LFTYHKHANTRTFVYIGAANYKTRVFLNGEKLGEHEGGFTPFNFEATSLLHDGENFLV
                     VEVNNARHVEDVPTVNTDWWNYGGITGDVSLVEVPDAFIQDYFVQLAKGSRDQVAGWI
                     QLNCA"
BASE COUNT          951 a         1355 c          984 g          802 t
ORIGIN      
        1 tctggaggat tccatggcta ccaagactct catcggcccc agcatcaaga ccgctcttcc
       61 cggccccaac gccgcccgcg tgctcgccgg agacgaaaaa tatatttcgc cgtcctacac
      121 ccgttcctat ccgctcgtag ccaagagcgg acgcggcctt accgtcacgg acgtggacgg
      181 caatgagttc ttcgacttct ccgcgggcat cgccgtaaca tccaccggac actgccatcc
      241 cgaagtcgtc gccgccatcc agaaacaggc cgccgaactc atccacatgt ccggcacgga
      301 cttttattac cagagcatgg tcacatttgc cgagcgtctc tcgaagatcg cccccatggc
      361 cgggccgcat aaaatctatt acggcaactc cggcgcggaa gcgattgagt gtgcccttaa
      421 gctggcgcgt tatcacacca aacggcagaa catcattgcc ttctacggcg cgtttcatgg
      481 acgcaccatg ggcgcgcttt cgctcaccgc ttccaagccg caacagcgcc gccgtttcgc
      541 gccgctcgtt cccggcgtga cccacgtgcc ttatccaaac atttatcgcg actgcaaaaa
      601 tcccgacgac gccgaaaatt acgcgctcat ctgcgcccgc tttatcgagg aaaaattatt
      661 caagaccacg cttcctcccg aggaagtcgc cgccatcttc gtcgagccca tacagggcga
      721 aggcgggtac ttggtcgcgc ccacaatttt tatgcaggaa ctgcgccgca tctgcgaccg
      781 ccacggaatt ctgctggttg cggatgaagt ccaaagcggc gcgggccgca ccggaaaatg
      841 gtgggccgtc cagcacacgg gtgtcgagcc tgacatcgtc tgtatcgcca aaggcatcgc
      901 ctccggcatg ccgctcggca tcaccatgag ccgcgccgaa gttatggact gggtccccgg
      961 ctcccacgcc tccaccttcg gaggcaatcc cgtttgcctg gccgccgcgc tggctacgct
     1021 cgacgtgatc gagcatgagc gcctgctgga aaattccacc gaagttggca actacatgct
     1081 caagcgcctc gccgattggc ccgcgaaaca caaactggtg ggcgatgtcc gcggacgcgg
     1141 cctcatgatc ggcgtggaaa tcgtgaccga caaaaagacc aaagccgccg ccaccaaaga
     1201 gcgcgaccgc attctggagc tcgccttcga acgcggcgtc ttgttcctcg gatgcggccc
     1261 cagcaccgtc cgtatcgccc cgccgctcat cgtcaccaaa gaacaagccg acgtcgccat
     1321 ggacgcgctc gaagaatcca tcgcaattgt cgccaggagc gcttgaaact catctcctgc
     1381 cattttttgt gggagtaagt ttcagccatg tcatgttgaa cgagtaagtt tcaggcatgt
     1441 catcccgacg ttgagcgaag cgaagacgga gggaccttac gtcgacaaag gccgccaatg
     1501 ctgctgacaa gacagtagtt cctgccagca gcaatgtctt ttgcaagcaa gcggggccaa
     1561 gcggtgagcc agaagcacat cccccaataa atgtcattct gagaagcaaa gcgacgaaga
     1621 atctctaccc gatgcacggc atttcgacct aggggtctcg tccgcgcaaa ccgttttcaa
     1681 acgtgagccc tgtgacatcc gtcacatcgc aaatcacaaa gccttagcta acgtagtccc
     1741 atgccttccg gccgcctaac ccacgccacc atccgagccc tcgactggct cgcagaaaag
     1801 accctccccc cgcaaaacat ccctccccac cagcgcaccg gacgccaggg cgaagaggct
     1861 gcctacttct acctccgccg cctcggatac atcatggtcg cgcgcaactt ccgctcgccc
     1921 caccgccgcg gcgaaatcga tctcatcgcc tgggagaaag acgtcctctg cttcatcgag
     1981 gtgaaaaccc gcaccaccca tgacgtaaaa cccgccgagg ccgccgtgga tgccgacaag
     2041 cagcgtgagc ttaactatat ggcgcgcgag tacttgcgcc acctgccacc ttcgtgccag
     2101 tggcgcttcg acgtgctcag cgtatattat gagcagccag cgaaatcccc gcggtttgag
     2161 ctgttccgca acgcaattcc gttgtcataa aatgaattct cgctgcgcgt tctggctgca
     2221 ctttcgataa gaagggattt gtacgcctga atcaaaatgc ctgaactaag aaaagatccc
     2281 atcgtcggcc gctgggtcat catctccacc gaccgcgcca agcgccccaa cgatttctcc
     2341 cgcgaggtca tccagctcaa gggcggcttc tgcccttttt gttacggcaa cgaagccaag
     2401 acccctccgg aaattcaggc ctaccgcccc aaccacaacg gaggcccgcc gccgcagcgc
     2461 gatactcccg gctggaccgt ccgcgttgtc cccaataaat ttcccgcgct cggcattgaa
     2521 ggcagcttaa accgccaggc cgaaggcatg ttcgacaaga tgaacggcat tggcgcgcat
     2581 gaagtaatta ttgaaactcc cgaccacgcc accactctcg ccatgctggc cccaaagcgc
     2641 atcgaagaca tcctgtggac cttccgcgat cgcattctcg acctgaaaaa agaccgccgc
     2701 ttcaaataca tcctcacgtt caagaaccac ggcgaggccg ccggagcttc gctcgagcac
     2761 tcccattcgc agctcattgc cctgccaatt cttcctattt acgtcgccga ggaactcgaa
     2821 ggcgcaaagc aatattttga ttacaaagag cgctgcgtct tttgtgacat cattcatcag
     2881 gaaaccgaag cgggcgttcg cgtggtcgcc gaaaatgaag acttcctcac catcgctccc
     2941 ttcgcgccgc gttttccctt tgaaacctgg gtccttccca agcagcacga gtccgccttc
     3001 gagcactcgt catctcacat gttcgagaac ctcgccaagg cacttaaact tctgctcaac
     3061 aaagccgacc gcgtcctcga caatccgccc tacaacctgg tcattcatag ctctccagta
     3121 caggaaccca acaacgacta ctatcactgg cacatagagt tcatgccgaa attaaccaag
     3181 accgccggct tcgaatgggg caccggcttc tacatcaatc ccaccccgcc agaggaagcc
     3241 gcaaagtttc tacgtgaggc gaacgtggaa gagccaatca ccgccacagt tcgctgaatt
     3301 ttcaatcgcc agtcgtcttc gtatagaaat ctcccagcaa tgtcatcctg agcgaagcga
     3361 aggacctatg caccccggcc tcctgcattc caattccaat cttccactac aatagctgtc
     3421 gccatgaaac gactgctctc gtttttcatt ctgctatcgt ccctatgctt cgccgcccca
     3481 gcgcccagca atgtaatcgc aaacatcccg ggccgcactg cggtttcgct gaatggcgca
     3541 tggaacatca tcattgatcc gtacgaaagc ggactcggct cgcgctacta cctcaacgcc
     3601 aagccaaaag acaagagcga tctggtcgaa tatgacttcg acaaatctcc cacgctaaaa
     3661 gtgcccggcg attggaatac ccagcgagac gagcttctat tttatgaagg ccccatttgg
     3721 tatcgccgct tgttcaccta tcacaaacac gccaacacgc ggaccttcgt ttacatcggc
     3781 gcagccaact acaagactcg cgtctttctg aatggtgaaa aactgggcga gcacgaaggc
     3841 ggattcactc ccttcaactt cgaagccacc agccttctgc acgacggcga aaattttctc
     3901 gtcgttgaag taaacaacgc ccggcatgtg gaagatgtcc ccaccgtcaa caccgattgg
     3961 tggaactacg gtggaatcac cggagacgta tcgctggtgg aagtccccga tgctttcatt
     4021 caggactact tcgttcaact cgccaaagga tcgcgcgacc aagttgccgg atggatacag
     4081 ctcaactgcg ca
//
LOCUS       DATNDT010000532         3104 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_3363328, whole genome shotgun sequence.
ACCESSION   DATNDT010000532 DATNDT010000000
VERSION     DATNDT010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3104)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3104)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3104
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3363328"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="VN788_17390"
     CDS             <1..223
                     /locus_tag="VN788_17390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22356.1"
                     /translation="RIRRKKAFEEAAKVRKIYEELVLKKKSEPLMQIGTMDEILTAVP
                     GATVKRAEPPNDADATEAVTSEPATVAGD"
     gene            365..1027
                     /locus_tag="VN788_17395"
     CDS             365..1027
                     /locus_tag="VN788_17395"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015199813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HXU22357.1"
                     /translation="MPDRKRARELAAEFIQKGDPVGWFEKLYQEAERGEATVPWNDLE
                     PNPRLLDFWKTHPSETAGKRALVIGSGFGDDAEQLAGWGFKTIGFDVAKTAIEAAKKR
                     FPQTQVEYVVADLFDPPADWLRGFDFVLEIYTVQSFPAEMQSKAIEAISQFVAPGGLL
                     LVIARGRADDEPEAQGPPWPLTRDAFNCFLRAGLREESFEDYAEPEPPWVRRFRALYR
                     RP"
     gene            1260..>3104
                     /locus_tag="VN788_17400"
     CDS             1260..>3104
                     /locus_tag="VN788_17400"
                     /inference="COORDINATES: protein motif:HMM:NF014759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HXU22358.1"
                     /translation="MPAKNTKRLFLVDAMGYIFRAFFAPMDRLQTSSGIPTKVPYLFA
                     TMMRRLINNPALRPDFVAVVFDVSAPTFRDEIFAAYKAQRAPMPDDLSIQIPFVRRYC
                     EAMRLPILEYPGYEADDVIGALALQAEKEDLDVYIITSDKDLMQLVHGRVRLLNPAKN
                     DLIVDEKKVEEIMGVPPEKVPDVMALMGDSIDNIPGARDPNDKPAPGERRKAGIGEVG
                     ARQLIQQFGSAEEAVARAAEVKRASYREALEKCGEFVRLSKRLAKIPTDAPVPLSLDA
                     LQLQEPDAVALRALYAELGFTSLLKEVAAPVETRVTDYAALESPEALREFAIGAAAAG
                     DAAVWLALDAEDPDDEGFGTRVAGLEMSIRADAARTAMNDAENKMLGVMHDWLRDANR
                     SKVVHDPKLFHLLASGSAANGAKPIAGIRHATMLYSYLIRPTTANHAFAEVVLRHLNR
                     TLSGAPGERADFLLRLAPVLRAEVEKHGLVDVYEKIDLPLAPVLARMEAAGVRVDRKE
                     LEIISTKAEEEITRLEKSIYELAGFEFKINSPQQLAEVLFDKLNLQAPRRSRAKSRST
                     AAEVLDELALIHELPRKVLEYRELAKLKSTYADALPRLINAETGRLHTR"
BASE COUNT          670 a          847 c          985 g          602 t
ORIGIN      
        1 tcgcatccgg cgcaagaaag cgtttgagga agcggccaag gtgcgaaaga tctacgagga
       61 actcgtccta aagaaaaagt ccgagccgct gatgcagatc ggcacgatgg atgagattct
      121 gacggctgtt ccgggcgcga cggtgaagcg agccgagccc ccgaatgacg ccgatgcgac
      181 tgaggccgtg acgtccgagc ctgcaaccgt ggccggcgac taaaaggcgc agctaccctt
      241 cgcgcaaaac gctcagggca aggcactcgc ccctacaacc tttaccgacg atttaccagc
      301 agcccccggc ttcggccggg gccttttttt ggttgtcggc cgaggtttcc aatagaatcc
      361 tgccatgccc gatcgaaaaa gagcgcggga gctggctgct gagttcatcc agaagggcga
      421 tccagtcgga tggttcgaga agctttacca ggaagcggag cggggagaag cgaccgttcc
      481 ttggaacgac ttggagccca atcctcgatt gcttgatttt tggaagacac atccgagcga
      541 gacggcgggc aagcgcgcgc tggtgattgg cagcgggttc ggcgacgatg ccgagcaact
      601 tgccggctgg ggattcaaga caattggatt tgacgttgca aagacggcca tcgaagcagc
      661 gaagaagcgg tttccgcaaa ctcaggttga gtacgtggtc gcggacctgt ttgatcctcc
      721 cgcggactgg ttgcggggct tcgatttcgt gctggagatt tacaccgtgc agtcgtttcc
      781 ggcggagatg caatcaaaag caatcgaggc gatttcgcaa tttgtagcgc ccggcgggct
      841 gctgctggtg attgcgcgag gccgcgcgga cgacgagcca gaggcacaag ggccgccttg
      901 gcctttgacg cgagacgcat tcaattgctt tttgcgtgcg ggattaagag aagagtcctt
      961 cgaggattac gccgagcccg agccgccgtg ggtgcggcgc ttccgagcgc tctaccggcg
     1021 gccctgaacg cgtgttttgg cctctcagac taatcgccgc ccgtgttggc gcaagcggat
     1081 gtgctacttc cagccaagta tctttggccc ttcgcaaacc gcgctgagtg taaacgggag
     1141 tagcagtgct acgcccctac gaaggcaaag gcattggttt gatatactgg accttagcga
     1201 tccgcttccg ttccaaaaga caattccgcc gaacggtccc ggccgaaaaa taatttcgca
     1261 tgcccgctaa aaacacaaaa cggctctttc tggttgacgc gatgggatac atcttccgcg
     1321 cgttttttgc gccgatggac cggctgcaga cgtcctccgg cattccgaca aaagtaccgt
     1381 atctatttgc caccatgatg cgacgcttga taaataatcc ggcgctgcgg ccggattttg
     1441 tagcggttgt attcgacgtt tccgcgccca catttcgtga cgagattttt gccgcctata
     1501 aggcgcagcg cgcgccgatg ccggacgatc tttcgatcca gattccattt gtgcggcgtt
     1561 actgcgaggc gatgcggctg cccatcctcg aatatccggg atatgaggca gacgacgtga
     1621 tcggcgcgct ggcgttacag gccgagaagg aagacctcga cgtctacatc atcaccagcg
     1681 acaaggacct gatgcaactg gtgcacggtc gcgtgcggct gctcaatcct gcaaagaacg
     1741 acttgatcgt ggacgagaaa aaagtggaag agatcatggg cgtcccgccg gaaaaagtgc
     1801 cggacgtgat ggcgctgatg ggtgattcga ttgacaacat tcccggggcg cgcgatccca
     1861 atgacaaacc cgctccaggc gaacgacgaa aagcgggaat cggagaagtg ggcgcgcgcc
     1921 agctgattca gcaattcggg agcgccgagg aggccgtagc gcgcgccgcg gaagtgaagc
     1981 gggcgagcta tcgcgaggca cttgaaaaat gcggcgaatt cgtgcggtta agcaagcgac
     2041 tggcgaaaat tcccactgac gcgccagtgc cactttcgct ggatgcgctg caactgcagg
     2101 agccggacgc ggtggcgttg cgtgcgctct acgccgagct gggatttaca tcgctgttga
     2161 aggaagtggc cgcgcctgtg gaaacgcgcg tgacggacta tgccgcgctg gaatctccgg
     2221 aggcacttcg tgaatttgcg attggcgcgg ctgcggccgg cgatgccgcg gtgtggctgg
     2281 cgctcgatgc cgaggatcct gacgacgaag gcttcgggac gcgggtagcg ggactcgaga
     2341 tgtccatcag ggccgacgcg gcgcgtacag ccatgaacga tgccgaaaac aagatgctcg
     2401 gcgtgatgca cgactggctg agagacgcga atcggtcaaa agtggtccac gacccgaaac
     2461 tttttcattt gctggcctct ggcagcgcgg cgaacggggc caagccgatt gccgggattc
     2521 gccacgccac gatgctttat tcgtatctga ttcgtcccac gacggcgaat cacgctttcg
     2581 cggaggtggt gctgcgccat ttgaatcgga cgctttcggg cgcgccgggc gaacgcgcag
     2641 attttctctt gcggcttgcg cccgtacttc gggcggaagt tgaaaagcac ggactggtgg
     2701 atgtttacga gaaaatcgac ttgccgttag cgccagtgct ggcgcgcatg gaagcggccg
     2761 gggtgcgcgt ggatcggaaa gaattggaga ttatttccac aaaagccgag gaagaaatta
     2821 cgcggctcga aaaatcgatt tacgaactgg cgggttttga gttcaagatc aactcgccgc
     2881 agcaacttgc cgaagtcttg ttcgataagt tgaatcttca agcgccgcgc cggagccgcg
     2941 cgaagtcgcg atcgacggcc gcagaagtgc tggacgaatt ggcgctgata cacgaactgc
     3001 ccaggaaagt tctcgaatac cgcgagcttg ccaaactgaa atccacgtat gcggacgcgt
     3061 tgcctcggct gatcaacgcg gagaccgggc gcctgcacac ccgc
//
LOCUS       DATNDT010000533         3885 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2753750, whole genome shotgun sequence.
ACCESSION   DATNDT010000533 DATNDT010000000
VERSION     DATNDT010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3885)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3885)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3885
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2753750"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VN788_17405"
     CDS             <1..945
                     /locus_tag="VN788_17405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HXU22359.1"
                     /translation="LAQVRQVIVGQDEVLDQILTALFVGGHCLITGLPGTAKTLMVRT
                     IAQTLGLVFKRIQFTPDLMPSDITGTDIIEEDPTTGHRRWTFVHGPIFGNILLADEIN
                     RTPPKTQSALLEAMQELSCTVRGNQYTLPSPFFVLATQNPIELEGTYPLPEAQLDRFF
                     FNIVLDYLSAEEEVKVVDLTTTTHTAQVQAITNAGEILDFQQLVRMVPVAESLAAYIV
                     KLVRATRHNSGVAPDFVKKYVNYGASVRAAQYIVLASKARALARRRYHVAYEDIAAVA
                     IPVLRHRVLLNFHAESDRIDSDEILRQLLQQVPPPQGA"
     gene            942..1835
                     /locus_tag="VN788_17410"
     CDS             942..1835
                     /locus_tag="VN788_17410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HXU22360.1"
                     /translation="MSMQRFLDPAVLSRISGLDLIAKTVVDGFVAGLHRSPDFGFSQE
                     FAEYRAYSPGDDLRHVDWNVFARTERAYLKRYRGETNSLLTVLLDASNSMTYTSHGVT
                     KMDYARFVAASLFYLAIHNQRDAAGLIVFDDEVRNYIRPSIRRGQLYRLLSGLELAEA
                     RARTDFAKPLQHFQQFLRRRGVVLIISDFFEAPQEIVRTIEPLRFHGNEVVLFHVLDP
                     KEIRPELGEPAILVDLETQQRIEVTPEYAKNEYRAKMDAHISELRERTQGAGMDYFLL
                     VTDRPLDSALAEYLTIRLGRN"
     gene            1836..3851
                     /locus_tag="VN788_17415"
     CDS             1836..3851
                     /locus_tag="VN788_17415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HXU22361.1"
                     /translation="MGVLAPWFLAGAAAIAIPIYLHLLRQHTSAPHPFSSLMFFEPRT
                     QTSIRQRRLRYLVLLALRLALLIFLVLAFADPFINRSAAGVKSEKLMMVVVDNSFSMR
                     AGSRLADAKREAISLLASRNPAERAQVMTLGAKLEALTQPIQDSGALRAAVQSIAAGD
                     SRGNFGELARSLRSLATNVPTPIELHLFSDMQKSNMPPTFSDLALPGNVKLVLHPVAN
                     GNPLPNWAVEGVNAPGQVWQPKKARVQAVIAGYNTHEGTRTVSLVVNGKTIATKKVAV
                     PANGRATVEFDSLDVPYGFSRCEVRIDSADVLPGDDATLFAVERSDPEQVLFLHDATD
                     TRSPLYFGAALAASAQSAFTMESVPVLQAGSIQFSKYAFVVLSDAGSLPASMENDLSQ
                     YVRRGGSVLIAAGTSLGRRSRIPIFGANILQARYYSNGGGSYLMVGDADPSFPSIAKA
                     DRWSGVKFYYATQVDDSGSHVVARLADHTPLLSEKRIGEGRVLLLASGFDNVTNDFPL
                     HPQFVPFVEQTAFYLSGTQRRSGSRAVDSFVELRNAKEQAVGIEVIDPDGRRPLSLKD
                     AATAQSFQLTRAGFYQFRLASGRQDVVGVNPDRRESDLVAMSPDVQSLWSGNAGTQPE
                     HAVVADEAPDKNARFPLWWYVMLLALAVALAESWVANQHLAARGEDL"
BASE COUNT          747 a         1240 c         1067 g          831 t
ORIGIN      
        1 ctcgcgcagg tccggcaggt gatcgtcgga caggatgagg tcctcgatca gatcctcacc
       61 gcgctattcg tcggcggaca ttgcctgatt acggggctgc ccggcaccgc caagacgctg
      121 atggtaagga ccatcgcaca gacgctcggg ctcgtattca agcgcatcca atttacgccg
      181 gacctgatgc cctcggatat caccggcacg gacatcatcg aggaagatcc caccacgggc
      241 catcgccgct ggacgttcgt ccacggaccg atcttcggga atattctttt ggcggatgaa
      301 ataaaccgca cgcctcccaa aacacaatcg gcgctgctcg aagccatgca ggaattatcg
      361 tgcactgtgc gcggcaatca gtacacgctg ccatcgccgt ttttcgtctt ggcgacgcag
      421 aaccccatcg agctcgaagg cacgtatccg cttcccgagg cgcagctcga ccgcttcttc
      481 ttcaatatcg tgctcgatta tctgagcgcg gaagaagagg tgaaggtggt cgatctgacc
      541 acaaccactc acaccgccca ggtgcaagca atcaccaacg ccggagagat tctcgatttt
      601 cagcaactcg ttcgcatggt gcctgtggct gaatcgctgg ctgcgtatat cgtcaagctg
      661 gtgcgcgcga cgcgccacaa tagcggcgtc gctccggatt tcgtgaagaa gtacgtgaac
      721 tacggagcca gcgtgcgcgc cgcgcaatat attgttctcg cttcgaaggc gcgcgcgctt
      781 gcaaggcgcc gctaccacgt ggcctacgag gatatcgccg ccgtggcgat tcccgtcctc
      841 cgccaccgcg tgttgctaaa ttttcacgca gaatcggatc gcatcgattc ggacgaaatt
      901 ctccggcaat tgctccagca ggtgccgcct ccacagggtg catgagcatg caacgctttc
      961 tcgatccggc tgtgctgtcg cgcatttcgg gcctcgacct gatcgctaag acggtcgtcg
     1021 atggtttcgt ggccggcctt caccgctcgc ccgatttcgg cttcagccag gagttcgccg
     1081 agtaccgcgc ctactccccc ggtgacgatc ttcgccacgt ggattggaat gtcttcgcgc
     1141 gcaccgaacg cgcctaccta aagcgctatc gtggcgagac caacagcctg ctcactgtgc
     1201 ttctcgacgc cagcaactcc atgacctaca cgtcgcacgg cgtcaccaag atggattacg
     1261 cgcgcttcgt cgctgcttcg ctcttctatc ttgccataca caaccagcgc gacgccgccg
     1321 gcctgatcgt cttcgatgac gaggtgcgca attacatccg accctcgatt cgtcgcggac
     1381 agttgtatcg cctcttaagc ggcctcgaat tggccgaggc tcgcgcgcgc acggatttcg
     1441 ccaagccgct ccagcatttt cagcagtttc tcaggcgccg cggagtggtt ttgattattt
     1501 ccgatttctt cgaagcgcct caagagattg tgcggaccat cgagcctctg cgatttcacg
     1561 gcaatgaagt tgtgctcttc catgtcctcg atccaaagga gattcgcccc gaactcggcg
     1621 agcccgcgat actcgtggac ctcgaaaccc aacagcgcat cgaagtgact cccgaatacg
     1681 ccaagaacga gtaccgcgcc aagatggacg cgcacatctc cgaattgcgc gaacgcacgc
     1741 agggcgctgg catggactat ttcttgctcg tcaccgaccg ccctctcgat agcgctcttg
     1801 cggaatacct cactattcgt ctggggagaa actgaatggg cgttttggct ccatggtttc
     1861 tggcgggtgc tgcggcgatc gcgattccga tttacttgca cctgctgcgt caacatacct
     1921 cggccccgca cccatttagt tccctgatgt tcttcgagcc tcggacgcaa acctcgatcc
     1981 gccagcgccg cctgcgttac ttggtgctgc tggcgctcag gctcgcactg cttatttttt
     2041 tggtgctggc ctttgccgat ccgttcatca atcgctcggc tgccggcgtg aaaagcgaaa
     2101 aactgatgat ggtggtggtc gataattcct tcagcatgcg cgccgggtcg cgtctagccg
     2161 acgcaaaacg agaagccatc tcgctgcttg cgtcgcgaaa tcctgccgaa cgcgctcagg
     2221 tgatgactct cggagccaag ctagaggcgc tcactcagcc gattcaggac tcgggcgccc
     2281 tgcgcgccgc cgttcaaagc atcgcagccg gcgattcccg cggcaatttc ggcgagctcg
     2341 cgcgctctct gcgatccctg gctacgaatg ttcccactcc gatcgaactt cacctcttca
     2401 gcgacatgca aaagtccaac atgccgccaa cttttagcga cctggcgctt cccggcaacg
     2461 tcaagctggt gctgcatccg gtggcaaacg gaaatccgct gcccaattgg gcggtggaag
     2521 gcgtcaacgc tcctggacaa gtctggcagc cgaaaaaagc gcgcgtgcag gctgtgatcg
     2581 ccggatacaa cacgcacgaa ggcacgcgta ccgtctccct cgtggtcaac ggcaaaacaa
     2641 ttgccacaaa aaaggtcgcc gtgcctgcca acggccgcgc caccgtcgag ttcgattcgc
     2701 tggatgttcc ttacggtttc agccgctgcg aagtgcgtat tgattccgcc gacgtgttgc
     2761 caggcgacga cgcaacgctc tttgccgtcg aacgctccga tccggaacag gtcctctttc
     2821 tccacgacgc cacggatact cgctcccctc tctatttcgg cgcggcgctg gcggcatcgg
     2881 cacaatctgc tttcacgatg gaaagcgtgc ccgttcttca agccggaagt attcaatttt
     2941 cgaaatacgc atttgtagtt ctttcagacg ccggttccct gccagcttca atggaaaatg
     3001 atctgtccca atacgtccgg cgcggcggca gcgtcttgat cgcggcggga acttcgctgg
     3061 gacgccgttc gcgcatcccc attttcggcg caaacatttt gcaggctcgc tactattcga
     3121 atgggggcgg cagctatctc atggtcggcg atgccgatcc atcctttccc tccattgcaa
     3181 aggccgatcg ctggtccggc gtaaagtttt actacgccac gcaggtggac gactccggtt
     3241 cccacgtggt cgctcggcta gcagatcaca caccgctgct aagcgagaaa aggatcggtg
     3301 aaggccgcgt tctgctgctc gcctcaggct ttgacaatgt caccaacgac ttcccgcttc
     3361 atccgcagtt cgtccccttc gtcgagcaga ccgctttcta tctttctggc acccagcgcc
     3421 gcagcggttc gcgcgcggtc gattccttcg tcgaattgcg gaatgcgaaa gagcaggccg
     3481 tcggcatcga agtgatcgat cccgatggcc gccgccccct ctcgctgaaa gacgccgcca
     3541 ccgcacaatc ctttcagttg acccgcgctg gcttttacca gttccgcctg gccagcggcc
     3601 gccaggatgt cgtcggcgtg aatccggacc ggcgcgaatc cgatctcgtg gccatgtcac
     3661 cggacgtcca atcgctatgg agtggaaatg caggcacgca gccggagcat gccgtcgtag
     3721 cggatgaggc ccccgataaa aatgcgcgct ttccgttgtg gtggtacgtt atgctgttgg
     3781 cattggccgt ggcgttggcc gaatcctggg tagcgaacca gcaccttgcc gcccgtgggg
     3841 aggatttatg agcgcaagaa acgagctcaa ctcctacgtg agtca
//
LOCUS       DATNDT010000534         3771 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_2609077, whole genome shotgun sequence.
ACCESSION   DATNDT010000534 DATNDT010000000
VERSION     DATNDT010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 3771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3771
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2609077"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..195)
                     /locus_tag="VN788_17420"
     CDS             complement(<1..195)
                     /locus_tag="VN788_17420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXU22362.1"
                     /translation="MERHIRSGERRSGFTLIELMIVMAIIGILLSIALPIYNKSIIRA
                     KESVLRNNLFTLRNVIDEYTY"
     gene            complement(170..637)
                     /locus_tag="VN788_17425"
     CDS             complement(170..637)
                     /locus_tag="VN788_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HXU22363.1"
                     /translation="MGSSRQDGFTLIELIVAFTILSILAVMAVPLARYRVRQQKEIQL
                     TNDLVEMRKAIDKYKDECDKNTFGPPKMDSDCYPESLEILVKGQKKASDANGKKIKFL
                     RRIPKDPFTGDTDWGKRSDQDDPDSDSWGGQNVFDVYTKTMEKAADGTPYSQW"
     gene            complement(643..3009)
                     /locus_tag="VN788_17430"
     CDS             complement(643..3009)
                     /locus_tag="VN788_17430"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HXU22364.1"
                     /translation="MPSFNYFKGFASIRGIALLAVLLCLAAGDSAARTRKGDKFLKQA
                     QKAEDSHKYDESAGLYDQALDQDPTDVGYQLGSRRAHFEASQDHLKKGQKLRASGQLQ
                     PALVEFQRAFSLDASSAIALEEMKTTMDMIDRNKKGNIPAAEQTLTPVQQEQKKINTR
                     LNELQDVPRLKPISNQMRALKMNNQPPRVLYETVGKLAGVNVIFDPQFSAKDKADLDL
                     GNTTLEQALDYIALLTHTFWQPISSNAIFVAEDNATKRRDYEDQVAKVFYLHNISTTQ
                     ELQEVVNAVRTVTDLRRIFQYAALNAVIVRGNVGQVALAEKLFHDLDKPKSEVEVDVI
                     ILNANRSRTRNLTASIIDAAAGAAGLGVPIAFTPRSFLQPSGTTTGTGTGTGTGTGTG
                     TGTGTGTGGGQSASPIVLGNIGKLSFSDFSTILPNAMLQAVMTDNSTKVLQSPQIRVS
                     DGQKATLKIGQKYPYATGSFQPGIGTVGVSPLVSTQFQFADLGVTVEMTPHVNGNDQV
                     TLHVAITIASHDGDITNSGLTQPILGNKQDETDIRLKDGEVSLLGGLLNTEVDSNVAG
                     IPGLVNIPVLGHFLFGAENKTNQRGELLIALIPHILRKPDLSPSDLRGVEAGTDQVVR
                     VSYAEPSEPPIPVPPPAPQPVLPSVTGEPKAEFSPGTIQARVGDPVPVTLQMRDAHDL
                     ASAPVKIKWDPNLLRLNQVSPGALFTQDGQKVNAPIDIRNDIGEATITMSRPNGAPGV
                     NGSGAVAVYQFVAVGHGSGNITVTELGAKNSKQESIPLSLPALPVAVQ"
     gene            3099..3584
                     /gene="smpB"
                     /locus_tag="VN788_17435"
     CDS             3099..3584
                     /gene="smpB"
                     /locus_tag="VN788_17435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HXU22365.1"
                     /translation="MEGKIAVKAQTGIKIISDNRQAGHNYHLMERFEAGLALTGTEVK
                     AAKDGKVQLKDSFAEVSGNEAWLVNAHISHYSHGNRQNHEPERRRKLLLHRKEIDELL
                     QLTREKGLTLIPTKFYLKGGRIKCELAVGRGKNLHDKREAERAREMEAEARAAVGRRR
                     Q"
BASE COUNT          778 a         1070 c         1105 g          818 t
ORIGIN      
        1 gtatgtgtac tcgtcgatca cgttgcgtag cgtgaacaga ttattacgca gcacgctctc
       61 cttcgcgcgg atgatcgact tgttatagat cggaagcgca atggacaaca ggataccgat
      121 gatcgccatc acgatcatca gttcgatcag cgtaaagccg gagcggcgct caccactgcg
      181 aatatggcgt tccatccgct gccttctcca ttgttttcgt gtaaacgtcg aatacgttct
      241 ggccgcccca ggagtccgaa tcgggatcgt cctggtcgct ccgctttccc caatccgtat
      301 cgccggtgaa aggatccttt ggaatgcgcc gcaggaattt gatcttcttg ccattcgcgt
      361 cgcttgcttt tttctgtccc ttcaccagga tttcgagcga ctccggataa cagtcgctat
      421 ccattttggg aggcccgaac gtgtttttat cgcattcgtc tttatactta tcgatcgcct
      481 tgcgcatctc caccagatcg ttggttagct ggatctcctt ttgctggcgg acgcgataac
      541 gcgccagcgg gacggccatc accgcgagaa tactcaggat cgtgaacgcc acgatcagct
      601 cgatgagagt gaatccgtct tgccttgaac ttcccatcgt cactactgca cggccaccgg
      661 caatgcgggg agcgagagcg gaatggactc ctgcttggaa tttttcgcgc caagttcagt
      721 caccgtgatg ttccccgagc cgtgcccgac ggcgacaaat tgatagacgg cgacagcgcc
      781 gctcccgtta acgcccggtg cgccgtttgg ccgcgacatg gtgatggtcg cctcgccgat
      841 gtcgttgcgg atgtcgatcg gagcatttac cttctgccca tcctgagtga acagcgcccc
      901 cggcgatact tgattgagcc gtaacagatt gggatcccat ttaatcttga ccggcgccga
      961 ggctagatcg tgcgcgtccc gcatctggag cgtcacgggg accggatcgc ctacgcgcgc
     1021 ctgaatcgtg ccaggtgaaa actccgcttt cggctcgccc gtgacggacg gcagcactgg
     1081 ttgcggcgct ggtggcggga caggaatagg aggctcggat ggctcggcgt agcttacgcg
     1141 gaccacctgg tccgtgccgg cctccacgcc gcgaagatcc gacggcgaaa gatccggctt
     1201 tcgcagaatg tggggaatca atgcgatcag cagctcgcca cgctgattcg tcttgttctc
     1261 cgccccaaac agaaaatggc ccaatacagg aatattgacc aaaccgggaa ttcccgccac
     1321 gttactgtca acctccgtgt tcagcagtcc gccgagcagg ctgacttcgc cgtctttcag
     1381 ccgaatatcg gtttcgtcct gtttgttgcc gaggatgggc tgggtaagcc cgctgttcgt
     1441 gatatcgccg tcgtggctgg cgatcgtgat ggctacgtgc aacgtgacct gatcgttgcc
     1501 attgacatgc ggcgtcattt ccaccgtaac gcccaaatcg gcgaactgga actgcgtgct
     1561 gaccagcggg ctcacgccca cggtcccgat tccaggctga aagctgccgg tcgcgtaagg
     1621 atacttctgg ccgattttga gagtcgcttt ctggccgtcg gagacgcgta tttgaggcga
     1681 ctggagaacc ttggtggagt tgtctgtcat tacagcctgc aacattgcgt ttggcaaaat
     1741 cgtggaaaaa tcgctgaagc tgagtttgcc aatgttgccg agcacgatcg ggctggcgct
     1801 ctgaccgcca cccgtgccgg tcccggtacc cgtgccggtc ccggttccag tccccgtccc
     1861 agtccccgtt ccggttgtgg tcccgctcgg ctgcaggaac gagcgcggtg taaacgcaat
     1921 cggcacgcca agcccagcgg cgccggcggc cgcgtcgatg atactcgccg tcagattccg
     1981 agtccggctg cgattcgcgt tcaggatgat gacatccact tcgacttccg atttcggctt
     2041 atccaaatcg tggaaaagct tctcggcgag cgccacctgt ccgacatttc cgcgaacgat
     2101 tacggcattc agcgccgcgt attgaaaaat ccgccgtaga tcggtgaccg tgcgcacggc
     2161 attcacaact tcctgcaatt cctgcgtggt gctgatgttg tgcaggtaaa aaaccttcgc
     2221 gacctgatcc tcatagtcgc ggcgtttagt cgcgttatct tcggcgacaa aaatcgcatt
     2281 cgaactgatc ggctgccaga aagtatgggt aagcagcgca atataatcga gcgcctgctc
     2341 aagcgtggtg ttgccgagat cgagatcggc cttgtccttg gcgctgaact gcggatcgaa
     2401 gatgacattg acgccggcga gtttccccac cgtctcgtac aacacgcgag gcggttgatt
     2461 attcattttc agcgcgcgca tctggttact gatcggcttc agccgcggca cgtcctgcag
     2521 ttcgttcaaa cgcgtattaa ttttcttttg ttcctgctgc accggggtca gagtttgttc
     2581 cgccgcaggg atgttgcctt ttttgttccg gtcgatcatg tccatcgtcg tcttcatctc
     2641 ttcaagagcg atggcggacg acgcatcgag cgagaacgct ctctggaatt caaccagcgc
     2701 cggttgcaac tgcccggaag cacgaagctt ctggcccttt ttcaagtggt cctggctcgc
     2761 ctcgaaatgg gcgcggcggg acccgagctg atagccaaca tccgtggggt cctgatcgag
     2821 cgcctgatcg tacaggccgg ccgattcgtc gtacttatgg gagtcctcgg ccttctgcgc
     2881 ctgcttcaga aatttgtcgc ctttccgcgt gcgcgcggcc gaatcgcccg ccgcgaggca
     2941 aaggagaacg gcgagcagcg cgattccccg gatggatgca aatcctttga aataattgaa
     3001 cgatggcatg ttagcgcgtg aagtaccccg tgataaactg aagctttacc gcacccggag
     3061 acactccata accgcttgac ggaatttggc ttgcgggcgt ggaaggcaaa atcgccgtga
     3121 aagcgcaaac cgggatcaaa atcatcagcg ataacaggca ggcgggccac aattatcatc
     3181 tgatggagcg ttttgaggca ggtttggcgc tgaccggaac cgaagtcaaa gcggccaagg
     3241 acggcaaggt tcaactgaaa gacagcttcg ccgaggtctc cggtaacgaa gcatggctgg
     3301 tgaacgcgca tatcagtcac tattcccatg gcaaccggca gaatcacgag ccggagcggc
     3361 gacggaagct gctgctgcat cggaaagaga tcgacgagct gctgcagtta accagggaaa
     3421 aaggcttaac cctgatcccg acgaagttct acctgaaggg cggacggatc aaatgcgagc
     3481 tggccgtcgg ccggggcaag aacctgcacg ataaacgaga agccgagcgg gcgcgtgaga
     3541 tggaagccga agcgcgggca gcggtgggca gaagacgaca gtagatggag gaaaacggaa
     3601 cgaatgcaag tggaattgaa agcgggacgt ccagaggaga tcgataccga cgcgctcatt
     3661 ctgatctctt tcgagggtgg aggcgaagcg cacggagaga ttgctggagg ctggctcagg
     3721 gagatccacg cgtcgggcga gttctccggc aaagcgctcg aaattgcgct g
//
LOCUS       DATNDT010000535        14736 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1332541, whole genome shotgun sequence.
ACCESSION   DATNDT010000535 DATNDT010000000
VERSION     DATNDT010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 14736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14736
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1332541"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            558..1568
                     /locus_tag="VN788_17440"
     CDS             558..1568
                     /locus_tag="VN788_17440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU22366.1"
                     /translation="MCKVSIVVPNYNHARYLPKRIETILRQTYQDFELILLDDCSTDD
                     SRRVLSRYEDNPRVRLEFNDVNSGSPFKQWNRGVCLARGAYVWIAESDDYADERFLER
                     LVPSLERDESIVYAYCRSCRVTEDGQLSGFEDTYIPHQGPRAWTGDYSVDGKRECLEY
                     FLFVNAVPNASAVLLRKSAYDLVKGADESLVLCGDWKLWAALALTGRIEYLSEPLNYY
                     RNHGVTQRSRTGRKSVSVSESLRVVKWIMNRMDLCTSECNAIYKAHAAGWVPELLSMH
                     VPLRVKATVWHDAKAMDPHPIRRIVIPAIETTRKSFVRRYKLVRSGFANPAALPRKSG
                     HA"
     gene            1561..2682
                     /locus_tag="VN788_17445"
     CDS             1561..2682
                     /locus_tag="VN788_17445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22367.1"
                     /translation="MRDAIVFSTWSWDMFNVPERIALAIAMRGARVLYCEMPGSRFKK
                     KGGSIGEVADGVHGFRPEYWGAKLNTFSPARKVQWRIVARQVLRKSDELELKDPIFIY
                     SHVKSIEPLCETMRGRGLPLVHVCMDYPEPYQYAQIALADLTLVIPKTVADKLRAEFG
                     DVKIALIPQIYHSPADDCHHAVDATAVHADLVKLKAQGRPILGYLGPLYGRLSVSLLK
                     ELLRLRPDWEFVCFGGADTLGLENAHDLPWVTAALLPSYLRVFDVGFMPYDCTDEKNL
                     HCSPLKIFDYFSLGMPVVATPVLSLRDFGNMVYLGETSRELARAVEMALNEPKDSEKR
                     QRRIEAVHSHTTQLVGERLEDILTFAEPRSRAVDSVTAW"
     gene            2724..2858
                     /locus_tag="VN788_17450"
     CDS             2724..2858
                     /locus_tag="VN788_17450"
                     /inference="COORDINATES: protein motif:HMM:NF028171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase C-terminal
                     domain-containing protein"
                     /protein_id="HXU22368.1"
                     /translation="MPVVMNASFNLRGEPIVCTPTDAVRTFFSSGMDALVIGNFVVEK
                     "
     gene            2863..3939
                     /locus_tag="VN788_17455"
     CDS             2863..3939
                     /locus_tag="VN788_17455"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU22369.1"
                     /translation="MQKWIALMGRRDQPTDGVADYCAFLAKPLSTSGVGLEPMHVSWD
                     SDGWLSAIYKLRRESKNWRGSTVLVQYTGMAWSRRGFPIGVMAVVRVLKHSHVRCIVV
                     FHEYERQSGSGRWIDRIRGACQDRVIRGLYRAAAKAIFTVPLKSVAWLPAERAKAQFI
                     PIGANVPERVNPRKPADASKTVVVFGVTGAPDMAPEVETIAASIRYAAEASPNLRLVL
                     CGRGSSEAGKLIEPLLQGSGAQVIAKGVLEADEIANELESADVHLCVRGPITLQRGSA
                     LAGIACGAPIVGYRNGRVCPPLDEAGIQWAPLGDSEFLARNLVKVLGDRRIWNDLHQR
                     NLRLQQNYFSWNRIAGQYLAALSQ"
     gene            3936..5129
                     /locus_tag="VN788_17460"
     CDS             3936..5129
                     /locus_tag="VN788_17460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012630290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU22370.1"
                     /translation="MNKRPVRVLFVASHPVQYASPIFRRLASDPRVEIQVAFCTLQGT
                     EAGVDPDFGVPVKWDVPLLEGFPWIAMRNLSPRPRVGSFFGLINTQVWSLIRRRRFDA
                     VVVYTGYMYFTFWIALVAAKLSGVPLLFGTDAHQLAPRDGKRWKLRVKGHVWPRLFKL
                     ANQVLVPSSGGVALMRSLGIPEDRIAFTPYVVNNDWWNERAKLVNRSEVRRRWNVPEQ
                     ALVIVFSAKLQPWKRPMDLLHAFARVPTPDVYLIFVGDGALRCDLESEAQSLGVADRI
                     RFLGFVNQSGLPETYSACDVLVLPSDYEPFGVVVNEAMLCGCCAVVTDRVGARFDLVR
                     EGETGFAYSAGDVDALARILTALAADPCTTKRLGEAARRQMETWLPQLGIEKTIEAVE
                     RSLVP"
     gene            complement(5233..5520)
                     /locus_tag="VN788_17465"
     CDS             complement(5233..5520)
                     /locus_tag="VN788_17465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22371.1"
                     /translation="MPWTKDPFAPVKKQSIRANRLISGMLDNSRFAKQPGQDEWRKGG
                     PCGVNDICIAYQACQSQLARATNDAKRCKGIVHVSGWGLSSNGNLKVIIFF"
     gene            5683..6318
                     /locus_tag="VN788_17470"
     CDS             5683..6318
                     /locus_tag="VN788_17470"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU22372.1"
                     /translation="MPNTEVIFHGVPTSETASTYAKDRSEPICFAYVGRLVVEKGVPV
                     LLHASSQLAEQGFDFRVKILGDGPERQNLQNISQQLRLGGRVEFLGSVPLKRVSEILG
                     RIDAIVMPSICEEVAGLTAIEQMMRGHLVIASGIGGLCEVVAATGLTFPPGDVGALAN
                     CMRRVLENPGIVAEFGNAARSRALALFNKDRMVEGHVSIYRKALNGNVLQS"
     gene            6503..7972
                     /locus_tag="VN788_17475"
     CDS             6503..7972
                     /locus_tag="VN788_17475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22373.1"
                     /translation="MRRLFFIAIAVAGCAAAVAILNNWRKGFYIFFAWMMLEDLVRKY
                     TGNNLFLFFGKDILLALIYFSFFIACRKKRERVFRPPFVLFLSLFFWLGVLQIFNQNS
                     PSILYGLLGIKMYFYYVPLMFIGYSLIRNDEDIRRFLVLNAILAILVSCIGITQSVVG
                     NSFLNPATLAPDLQELGNLQKVTASGQAFNLPDSVFVSSGRYAAYLSIAFSVTMGGAG
                     FLLLYKKNNRKLIFIALGLIGVAALMSGSRGTFVTILMTAGLLSIGFIWGAPWRSRRA
                     NHVIKAIRRSVLAVAAAVVLMILVFPQQAGTRLEFYTDTLLPNSSDYQLGLRSWDYPL
                     QNLLKALDDPNWLWGNGIGTTALGIQYVQKIVGRSVLPPSVEEGFGTMIVEMGIIAPF
                     LWILWSASLLYFAWKIARGLRQTRFFPIAFSIAWYCFVLLFLWTWGGLDAYENYTCNI
                     FLWLLIGVLFALPDIARKSDLAIEVNDTTRHDHLVYQQG"
     gene            8046..9266
                     /locus_tag="VN788_17480"
     CDS             8046..9266
                     /locus_tag="VN788_17480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU22374.1"
                     /translation="MRGKPRIAVVSPFLDKSHGTERILIEWFSRLGDDFELHIYSQRV
                     DDFDRSQFIYHRIWELPGPHILNYVWWFLANHIRRGWDSAFGNLTPDLVFTPGINCMD
                     ADVISIHVVFAQLCRQARPELRFSANPLRRWPRLLHRRLYYSLVVFLERRIYSNPHTQ
                     LVPMAKKTQADLNRFYARDGGCPILHLGLEHARFNPSRRAELRQASRESLGLTGRDFV
                     ALLIGNDLLNKGMLVLFEALSRLPGLPLRLLIVSRESGNAYRSVLKEKRLADQVRFLS
                     LRKDVELYFAAADLYTGPSLEDAFAMPPQEAMACGLPVIVSRAAGVSEIITDGENGLI
                     LEDARDANALAAMIRRIYEDQAFRTALGQNAAKSVEQFTWERNAQDLRAIFEDVLRRK
                     RDSPSMRKVLEPDR"
     gene            9274..10449
                     /locus_tag="VN788_17485"
     CDS             9274..10449
                     /locus_tag="VN788_17485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HXU22375.1"
                     /translation="MPKYKSANAAVSASPAAPAHITGLFPELLGVGGVQEAGRLTALA
                     LQRIADRRGWSAAFVGLNDPVGPQQLFIAGHTILLRGVARAKIPFILAAMRSSRAARG
                     GGSHFVLAGHPHLAPIAVWMQKMLPRTHAVVIAHGVEVWQPLPVLRRTAIRQAHVVAG
                     PSSDTIQKLADVQAVHANRAHLLPWPLNPDFLSLTNTSNLPLPSGFPAGKVILTIARA
                     AASEQYKGTDDLILSVSQLRSEFPDLHLVAIGGGDDLPRLQKLANESGSANSVRFLQC
                     LSREELAACYARADVFAMPSVGEGFGLVFLEAMAFGKPVVAAACGGVLDLIRDGSNGL
                     LVPQRDLVALVSALRRLLGDDSLRASLGTEGATIAREKYRFDSFETNLEHLLKEGPI"
     gene            10551..11741
                     /locus_tag="VN788_17490"
     CDS             10551..11741
                     /locus_tag="VN788_17490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HXU22376.1"
                     /translation="MRVLKTVQSYYPFQDRGGPVVKVRALARSLAHRGHSISVLTADL
                     GLRNRSWDNLHIENCPFGWRAEEEGVEAIYLPTIGRFRALTINPGLMRFCDASLKQYD
                     LVHFYGLYDLLGPAVSHFCRRGRIPYVIEPMGMYRPIDRSFRLKRVWHRILGGPYVRN
                     ADKIVATSEIERQDLLEEGLPPEKIVIRYNGVEVDRFGAQGVRGSFRAKWAIPPADKL
                     ISFLSRLIPRKGADILIEAFAKACPHSGTLVIAGPEGQAGYVSHLKKYAEENRVANRV
                     IFTGPVYDADKAALYSDSDVFVLASRYENFANVVAEAVACDIPVIVSNMCGIHSLVDG
                     RAGIVTSVQRDSIAAALQRLLADEALYARLRAGCRDVAQELTWGTLSAQMENYYSEVL
                     AGSN"
     gene            11829..12761
                     /locus_tag="VN788_17495"
     CDS             11829..12761
                     /locus_tag="VN788_17495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22377.1"
                     /translation="MKARASASLSAHNSHIPGTVSNAAARDLREAGFGHGIFRVTWYG
                     VNILLFAALVGVLWTAAWEYSTECYLRGFSDAVIPFSAAPTRKIEAILHWMGHSPARF
                     DQAPESMADDRDPTDTLNYASLLSVCGTATNAFLNLADTGGLTVRRLLLLDVNRTTVH
                     VVAEVLIDGRWIIVDPVFHTIFRGTDGGMLTSQQLASPIVFRRATQNIPGYLPTYTFD
                     RAVHVRLSHFGPLGRWVRNIWGVPAWEGWPVLSLIMERESLELFVVFAFLVLCLLIVR
                     GVLRWYGEARLGIRMEHMYRKLFRAGRLLFEPTD"
     gene            complement(12768..12995)
                     /locus_tag="VN788_17500"
     CDS             complement(12768..12995)
                     /locus_tag="VN788_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22378.1"
                     /translation="MNEQANQQAAGERGHQCVCEELFGHVQDLFGVSPTVRQHLKNSR
                     IEFLKAVRSVIDERIDRMSKAESRGTRVAVD"
     gene            complement(12992..14182)
                     /locus_tag="VN788_17505"
     CDS             complement(12992..14182)
                     /locus_tag="VN788_17505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015446 - ATPase-coupled arsenite
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0071722 - detoxification of
                     arsenic-containing substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRC40/GET3/ArsA family transport-energizing
                     ATPase"
                     /protein_id="HXU22379.1"
                     /translation="MRILLFSGKGGVGKTSLAAATGLKLAELNYRTLVMSIDPAHSLA
                     DSFDLNTDLFHAQTSDPLPITNRLSIFELNIQKEIKRHWQEISSYITSVLRTTGISGV
                     EAEELAILPGMEELSAMMYINQYRREQTYDAIVLDAAPTAESMRFISMPTTLDWYMKH
                     IFPFQRNLLKAVRPIANRVAPFELPTDNYFVNIRNLFEKLEGVDDLMEDPKTTSVRLV
                     TNPEKMVLRETERAFVYFSLHGLTVDTVVVNRLLPSDVTDAWFTAWHKSQEGILREIE
                     EYFAPVPVKRVPLFEQEVLGKERLERLASLLYPSGEDPAAVTRTEKPYTFEKSDGHYQ
                     VRILLPFAAKGDVGLFKKGDELVIEIGTLRRHIGLPRSMAMLNPGRARLEHRVLTVEM
                     KEAQ"
BASE COUNT         3029 a         3794 c         4197 g         3716 t
ORIGIN      
        1 gtaggcgggt accagataga ttcgacgctg aggatatttt gcacgacgtt ctctacgggt
       61 tccttgagtc gggcaattca gcttacgagt cttcggagcg tgggcggtgg cgtgcgccac
      121 cgtggcccga gatgacatca gaacagcgag agcaattcaa gcaggaacta cgagcgggct
      181 gcgggcgtgt tgcgccgtcc cagccagcat ctcgcgcttg attgtgtaat ttgctgagac
      241 ccgctctgct cgacggcgtt cattgctgga gtacccccag ggtggcgcac tcagccgtgc
      301 tgttcgactt ttgcgcgaag tctttgcttg gcaaggacat actgatccga agaccttaca
      361 tgttggatat aaagctctta cagcatggag atacgattcg accaatatga gcgagtctcc
      421 tcggcgtcgc gctctcagcc accgcattgc ggaaagtatg tatttcgtca cgcaggtcat
      481 tcgtgttaac cacatcatgt tattgtcgtt agagagcatc gagcacaaag gtttagggga
      541 ttttccacca tcgacagatg tgtaaggtta gcatagtcgt tccgaactac aaccacgctc
      601 ggtatttgcc gaagcgaata gagactattc tgcgccaaac gtatcaggat tttgagctaa
      661 tcctgttgga cgactgttcg acagatgata gccgccgagt gctctcccgc tatgaagata
      721 atccgcgagt gcgactcgaa ttcaatgatg tgaattcagg aagccccttc aagcaatgga
      781 acaggggtgt gtgcctggcg cgcggcgctt atgtttggat cgccgagtcc gatgattacg
      841 cggatgaaag attcctggag cgtctggttc ccagccttga gcgtgacgag tcaattgtat
      901 acgcatattg tcggtcgtgt cgcgttacag aggacgggca gttgagcgga ttcgaggata
      961 cgtatatacc tcaccaagga ccgcgtgcat ggacgggcga ttattcagtt gatggtaaac
     1021 gtgaatgcct cgagtatttc ctgttcgtca atgctgtgcc aaatgcgagt gcggtgctgc
     1081 tgaggaaatc tgcatatgac ttggtcaagg gagcggatga gagtcttgtg ctgtgcggtg
     1141 attggaaatt gtgggctgcc ttggctctaa ctggtcgtat agagtatttg agcgagccgc
     1201 tgaactacta ccgtaaccat ggagtgactc aacgcagccg aaccggacgg aaatcggtga
     1261 gtgtctcaga aagtcttcgc gtagtgaaat ggatcatgaa ccgaatggac ttatgtacct
     1321 cggaatgtaa cgcaatctat aaggcgcacg cggcaggctg ggtgccggag ttgctgagca
     1381 tgcacgttcc gctgagagta aaggcgaccg tttggcacga tgcgaaagcg atggatccac
     1441 atcctattcg aaggattgtg attccggcca tcgaaaccac gcgaaagagc tttgtgcgac
     1501 gttacaagtt ggttcggtcg ggatttgcca atccggcggc tctgccgaga aaatcgggcc
     1561 atgcgtgacg cgatcgtttt ctcgacgtgg tcttgggaca tgtttaacgt tcctgaacgg
     1621 atcgcgctgg cgatcgcgat gcgaggagcc cgcgttcttt attgcgagat gccgggttcc
     1681 cgatttaaga agaagggcgg atcgattggt gaggttgccg atggcgttca cggattccgt
     1741 ccggaatact ggggagcgaa attgaatact ttttcgcccg ctcgtaaggt gcagtggaga
     1801 atagttgcgc gacaggtgct tcgaaaatcg gatgagttgg aattaaagga cccgattttt
     1861 atttactccc atgtcaagtc gattgagcca ctttgcgaga ctatgagagg ccgcgggctg
     1921 ccgctggtac atgtatgcat ggattaccca gaaccgtacc aatacgcgca aattgcactc
     1981 gcggatttga cgttggtgat tccaaagaca gtcgctgaca agctgcgagc ggaattcggc
     2041 gacgttaaaa tcgccctgat tccccaaatc tatcattcgc cggccgatga ctgtcaccat
     2101 gctgtcgatg cgactgcagt ccacgctgac ttggtgaagt tgaaggcaca aggtagacca
     2161 attctgggct atttaggacc tttatatgga cggcttagcg tcagcttatt gaaagagctg
     2221 cttcggttga gaccggattg ggagtttgtg tgttttggag gcgcggacac attggggctg
     2281 gaaaatgcgc acgatttgcc gtgggtgact gcagcgctgc ttccctcata tctgcgtgtg
     2341 tttgacgtcg gcttcatgcc gtatgactgc acggatgaga aaaacctgca ctgttcaccg
     2401 ctaaaaatat tcgattattt cagccttggc atgcccgttg tagctacgcc ggtgctttcg
     2461 ctgcgggatt ttggaaacat ggtctatctg ggcgaaacat ctcgggagct ggcgcgtgcg
     2521 gtcgaaatgg cgttgaacga gccgaaagac agtgagaagc gacagcggag aattgaggca
     2581 gtgcactcgc ataccacgca attggtagga gagcggctcg aggatatttt gaccttcgct
     2641 gaaccacgat ctcgagcggt ggactcggta acggcgtggt gaccgtgatt agtggcggag
     2701 ctgatcgggg tgccaggact ggcgtgcccg ttgtgatgaa cgcttccttc aatttgcgcg
     2761 gcgagccgat tgtttgcact cctacggatg cggtccgcac attcttcagc tccggcatgg
     2821 atgcgcttgt gatcggcaac ttcgtcgtgg agaaatagct ccgtgcagaa gtggattgca
     2881 ttaatgggca ggcgcgacca gccgacggac ggcgtcgcgg actactgcgc ctttctcgcg
     2941 aagcctctat cgacaagcgg agtgggcctg gaaccgatgc acgtctcgtg ggatagcgat
     3001 ggatggctgt cggccatcta caagctgcgg cgtgagagca agaattggcg cgggagcacc
     3061 gttctcgtcc aatacactgg catggcttgg tcgcggcgcg gctttccaat tggagtcatg
     3121 gccgtcgttc gcgtcctgaa gcatagtcat gtccggtgca tcgtggtttt tcatgaatat
     3181 gagcgccagt ccggctcagg gcgatggatt gatcgcattc gcggtgcatg ccaggatcga
     3241 gtgattcgcg gcctgtatcg cgcggcggcg aaggcgatct tcaccgttcc gctcaaaagt
     3301 gtggcttggc ttccggcaga gcgggccaag gcacaattta tccccatcgg cgcaaatgtc
     3361 ccagagcggg tgaaccctcg gaagcctgcg gacgcaagca agaccgttgt agtgtttggc
     3421 gttaccggtg cgcccgacat ggcgcccgaa gtggagacaa tcgcagcgag catccgatac
     3481 gctgcggaag catcaccgaa tctgcgacta gttctttgcg gccgaggttc ttctgaagct
     3541 ggcaaactta tcgaaccgct tttgcagggg agcggcgcac aggtgattgc aaagggcgtg
     3601 cttgaggcag acgaaatagc gaacgaactg gaatcggccg atgtccacct gtgcgtacgc
     3661 ggtccaatta cattgcagcg gggcagtgcg ctcgcaggta ttgcgtgcgg agcgccgatc
     3721 gtcggttatc gaaacggaag ggtgtgccct ccgcttgacg aggcgggtat tcaatgggcg
     3781 cctcttggcg attctgagtt tttggcgcgg aatctggtga aggtcttggg tgaccggcga
     3841 atatggaatg accttcacca gcgaaatctg cgcctccagc agaactattt ctcgtggaat
     3901 cggatcgccg gacagtatct ggcagcattg tcgcaatgaa taagcgccct gttcgagtgc
     3961 tcttcgtggc ttcgcatccc gtgcaatacg catcgcctat atttcgccgg ctcgcaagcg
     4021 atcctagagt cgaaatccaa gtggctttct gcacattgca gggaaccgag gcgggagtcg
     4081 atccggattt cggtgttcct gtgaaatggg atgttccgct gctcgaaggt ttcccgtgga
     4141 tcgcgatgcg gaacctttcg cctcgtccgc gagtcggctc cttttttgga ttgataaata
     4201 cgcaggtctg gagcctcatt cggcgccgac gtttcgatgc tgtagtcgtc tacacgggtt
     4261 acatgtattt cacattctgg attgctctgg tggctgccaa gttgagtggt gttccgcttt
     4321 tgtttggcac ggacgcacat cagctcgcac ctcgcgacgg aaagcgatgg aaattgcgcg
     4381 tcaaagggca tgtctggccg cggctgttca agttggccaa ccaagtcctt gttccgtcca
     4441 gcggtggagt tgcactgatg cgatcactag gaattcctga agatcgtatc gcgttcaccc
     4501 cgtacgtcgt gaacaatgat tggtggaatg agagagcaaa gttagtgaat cggtcggagg
     4561 ttcgcaggcg ctggaatgtg cccgagcaag cgctcgtgat cgttttctcg gcaaagctac
     4621 agccctggaa acgccctatg gacctcttgc acgcgttcgc tcgcgttccc acgcctgacg
     4681 tgtatctcat ttttgtaggt gacggcgctt tgcggtgcga ccttgaatcc gaggcgcagt
     4741 cgcttggagt tgcggacagg atccgctttc taggattcgt gaaccaatcc ggcttgcctg
     4801 agacgtattc tgcctgtgac gtgttggtct taccttcgga ttacgaacca ttcggcgttg
     4861 tggtgaacga ggccatgctc tgtggctgtt gcgctgtcgt aaccgaccgc gtgggtgcgc
     4921 gcttcgatct tgtgcgcgaa ggcgaaaccg gatttgcata ttctgctgga gatgtcgatg
     4981 cgctcgccag gatcttaacg gccctggcgg cggatccttg cacaacgaaa cggttaggcg
     5041 aggcggcacg ccgacaaatg gaaacgtggc taccacagct cggaatcgag aagaccatag
     5101 aagctgttga acgtagcctc gttccctgag ccgctgatga aagtgctgat ctatacccac
     5161 gcgtttgcgc cctcgaccgg tggagttgag acctatgtga tgttgctcgc agagggacta
     5221 tcccgccgct catcagaaaa agatgatcac tttgaggtta ccgttgctac tcaaacccca
     5281 gccggagaca tgaacgatgc ccttacaccg tttcgcatcg ttcgtcgccc gagccagttg
     5341 gctttggcaa gcttggtacg caatgcagat gtcattcacg ccgcagggcc cgcccttccg
     5401 ccactcatct tggcctggtt gcttcgcaaa ccggctgttg tcgagcattc cggatatcaa
     5461 tcgatttgcc cgaatggact gcttctttac gggcgcaaac gggtcctttg tccagggcat
     5521 ttcatggcgc ggagatatat agaatgcgtc cgctgcaata cggatgaatg ggggtgggct
     5581 agtagtctgc gtagcatgct cctgatgttt cctcgtcgct ggctgtgccg tcgcgtagct
     5641 tgcaatgtgg ggccaacccg tcatatggca aagaggagtg cattgccgaa caccgaagtg
     5701 attttccacg gcgttccgac atccgagact gcatcgacct atgcgaagga tcggagtgaa
     5761 ccaatctgtt tcgcatacgt cggacgcttg gtcgtcgaga agggcgtccc ggtcttgctt
     5821 cacgcatcga gccaacttgc tgagcaaggt tttgactttc gcgtaaagat tctcggggat
     5881 ggtcctgagc gccagaattt gcagaatatc tctcagcagc ttcgattggg cggtcgggtc
     5941 gagtttctag gttcggttcc gctgaaaaga gtttccgaga tcctcgggcg catcgatgcc
     6001 attgtgatgc cttctatttg cgaggaggtt gcgggattaa ctgcaattga gcagatgatg
     6061 cgaggacatc tggtgatcgc atccggtata ggaggtctct gtgaggtcgt ggcagctacc
     6121 ggattgacgt ttccaccggg ggacgtgggg gcccttgcaa attgcatgag aagagtactg
     6181 gaaaacccag ggatcgttgc cgagttcggc aacgcggcaa gatctcgagc tctagcctta
     6241 ttcaacaagg accgaatggt cgaaggacac gtctccatct acagaaaagc gctcaacgga
     6301 aacgtgttgc aatcttgatc atgtgtatgc ttgccgcgag agattgctta ggacattata
     6361 ggattcgcag gccgatcgga acatgacctt gttgaactta cgcgtccgca ccgcaaatac
     6421 ccagccttta attggagcgg gcttgctcct attcgctctg atagcggggt ggcagattgc
     6481 cggattcatt gtcgcggatg acatgcgcag gctgttcttc atagcgattg ccgtggctgg
     6541 ttgcgccgcg gcggtcgcga tcctcaacaa ctggcgaaaa ggcttttaca ttttctttgc
     6601 ttggatgatg ctcgaggatc tcgtcaggaa atacacgggg aataacctct ttttgttctt
     6661 tggcaaggac atacttctgg cgctgatcta tttctctttt ttcatcgcgt gtcgcaaaaa
     6721 acgtgaaagg gtttttcgac caccttttgt cctattcctt agccttttct tttggcttgg
     6781 tgtgcttcag atctttaacc agaattcgcc aagtattctg tacgggttat tgggcattaa
     6841 gatgtatttc tattacgttc cgttaatgtt catcggatat tcattgatta ggaacgacga
     6901 ggatatcaga aggttcctag tgctgaatgc catcttggca attctagtca gctgcatcgg
     6961 tataacgcaa tcggtcgtgg ggaatagttt cttgaacccg gctactcttg ctcccgattt
     7021 gcaggaactc ggcaatctgc aaaaggtaac cgcctccggc caagctttta atcttcctga
     7081 ttccgttttt gtcagtagcg gtcgttatgc cgcctatctt tcgatagcgt tttcagtcac
     7141 aatgggcggc gcggggtttc ttctgcttta caaaaaaaac aaccgcaagc tgatattcat
     7201 cgcattgggc cttataggag ttgctgctct gatgagcggc agccgcggca catttgtcac
     7261 tattctcatg actgccggat tgctatctat aggcttcatc tggggtgctc cgtggcggtc
     7321 ccgccgcgcg aatcatgtga tcaaggccat caggcgatcc gtgctcgctg ttgctgctgc
     7381 tgttgtcttg atgattttgg tatttccgca gcaggcgggc acgcgtttgg aattttatac
     7441 ggatactctg ctaccgaatt caagtgatta ccaactcggg cttcgctcat gggactaccc
     7501 cctgcaaaat ttattgaagg cacttgacga ccctaactgg ctgtggggca atggtatcgg
     7561 aacaacagcc ttgggcatac agtacgtgca gaagatcgtt ggcaggtccg tacttccacc
     7621 ctcggtagag gagggtttcg gcacgatgat tgttgaaatg gggataatcg ctccgtttct
     7681 atggattctt tggagcgctt ctctcctata ctttgcttgg aaaatcgccc gcggcctccg
     7741 tcagacgaga ttttttccaa ttgccttttc gattgcgtgg tattgctttg ttctgctgtt
     7801 cctttggact tggggaggac tggacgcgta cgagaattac acttgtaaca tatttctgtg
     7861 gctcctgatc ggagtccttt ttgcattgcc tgacatcgct cgcaaatctg acctggctat
     7921 tgaggtcaac gacactaccc ggcacgacca ccttgtgtat cagcaagggt gatcacggta
     7981 atgtgccatc tcgtaattat gatagtgcca aatatagaat gtaagtcact gtttggttat
     8041 atggaatgag gggcaagcct cgcattgctg tcgtgtcacc ttttctcgat aagagccacg
     8101 gaaccgagcg cattcttatt gaatggttct cccgtcttgg agacgatttc gagctgcata
     8161 tctacagtca gcgagtggac gatttcgatc gttcccaatt catctaccat cggatttggg
     8221 agttgccggg accgcacatt ctcaattacg tgtggtggtt cctagcgaat cacattcgac
     8281 gcggctggga ttcggcgttt ggaaatctaa cgccagatct ggtatttacg cccggaatca
     8341 attgtatgga tgcggatgtg atctccatcc acgtcgtttt cgcgcaactt tgtcgtcaag
     8401 cgagacctga actgagattt tctgcaaacc ctcttcgacg gtggccccga ttactgcatc
     8461 gtcggctcta ttattcgtta gtcgttttcc tcgagcgccg gatttattcg aaccctcaca
     8521 ctcaactggt tccgatggcc aagaagacgc aagcggacct aaaccgtttt tatgctcgtg
     8581 atggcggctg tccgattcta catttggggt tggaacacgc acgtttcaac ccgtcgcgcc
     8641 gcgcggaact gcgccaagcc tcccgtgaat cgctcggctt aaccggacgc gacttcgtgg
     8701 ctctccttat cggcaacgac ttgttgaaca aaggcatgct tgtgctcttt gaggcgctat
     8761 cccggctgcc tgggcttcca ttgcggttgc taattgtgag tcgggaatcg ggaaacgcct
     8821 atcgaagtgt gttgaaggaa aagagactcg ccgatcaggt gcgatttctt tcactacgga
     8881 aagatgtgga gttgtacttt gccgcagctg atctgtacac aggtccatcg ctcgaagacg
     8941 cgtttgcaat gcctccccag gaggcaatgg cctgtggttt gcctgtgatc gtatcgaggg
     9001 ctgcaggcgt ttcggaaatc attacggacg gcgaaaatgg gctgatcctc gaggatgcgc
     9061 gcgatgcgaa tgcgcttgcc gcaatgattc gtcgcatata tgaggatcag gctttccgca
     9121 ctgcgctcgg tcaaaacgcc gcgaagtccg tcgagcaatt cacttgggaa cgcaacgcgc
     9181 aggatcttcg cgccattttt gaggacgttc tgcgtcgcaa gcgcgattcg cccagcatgc
     9241 ggaaggtctt ggagcccgac cgatgagaat tctttgccaa agtacaagtc tgccaatgcg
     9301 gccgtgagcg cgagtcctgc tgcgcccgcg cacatcacgg gacttttccc agagctcctt
     9361 ggtgtcggcg gcgtgcagga ggcgggccgc ctaacagcac ttgcgctcca gagaatcgcc
     9421 gaccgccgtg gctggtccgc cgccttcgtt ggactcaatg accctgtcgg tccacagcaa
     9481 ctctttatag cgggtcacac tatcctgctg cggggggttg ctcgcgcgaa gattccgttc
     9541 attctcgccg cgatgcgaag ttcacgcgca gcacgaggtg gcggttcgca tttcgttctc
     9601 gccgggcatc ctcatctcgc accaattgcc gtctggatgc agaagatgtt gccgcgcaca
     9661 cacgcggtcg tgattgctca tggtgttgag gtgtggcaac cgttgccagt ccttcggcgg
     9721 accgccattc ggcaggcaca cgtcgtggct ggtccgagtt cggatacgat ccaaaagctg
     9781 gccgatgtac aggcggttca tgcaaaccgc gctcatctgc tcccgtggcc tcttaatcct
     9841 gacttccttt cccttacgaa cacttcgaat ttgccactac cgagcggatt tcctgccggc
     9901 aaggtgatcc tcaccatcgc ccgcgccgca gcgtccgagc agtacaaggg cacggatgat
     9961 ttgatcctct cggtctcgca acttagatcg gaattcccgg atttgcatct tgtggcgatt
    10021 ggcggcggcg atgatctccc gcgcctgcaa aaattggcga acgaatccgg gagtgcgaat
    10081 agtgtgcgtt ttttgcaatg cctgtcgcgc gaagagcttg cagcctgcta tgcgcgcgcc
    10141 gacgtcttcg ccatgccaag tgtgggtgaa ggttttggcc ttgtttttct tgaggcaatg
    10201 gcttttggca agccggtggt tgccgcagca tgcggtggcg tgctcgatct tatacgagac
    10261 ggttctaacg gtttgcttgt tccccagcgg gatttggttg cgctcgtcag tgcacttcgc
    10321 cgcttgcttg gagacgactc cttgcgtgcg tcgcttggca ccgaaggcgc caccattgcg
    10381 cgcgaaaaat accgcttcga ttcgttcgag accaatctcg aacatttact taaagaaggc
    10441 ccaatataaa ctcaaacgga ggataggctc cgcgataccg tagtattgtc tttaattgtc
    10501 agttcaaggt ttggctgaca tccggaaagc gtaagcgaat cctgggacgc atgcgagtac
    10561 tgaagacagt ccagtcctat tacccttttc aggaccgcgg cggccccgta gtcaaagtgc
    10621 gtgctcttgc ccgtagcctg gcacaccgcg gccatagcat cagcgttctt actgccgatc
    10681 ttggtttgag gaaccgttcg tgggacaacc tccatatcga aaactgccct ttcggctggc
    10741 gcgcagaaga ggaaggcgtt gaagcaattt atctgcccac gattggccgt tttcgcgcgc
    10801 tcacaattaa ccctggcctg atgaggtttt gcgacgcatc gctaaagcaa tacgacttag
    10861 tgcattttta cggtctatac gatctgctcg gccccgcagt aagccacttc tgccgccggg
    10921 gccgcattcc ctatgtgatc gaaccaatgg gaatgtatcg tccgattgat cgcagttttc
    10981 gcctgaagcg cgtgtggcac cgcatacttg gtggtcctta cgtgcggaat gccgacaaaa
    11041 ttgtcgcgac ctccgagatc gagcggcagg acttgctcga agaaggtctg cctcccgaga
    11101 agatcgttat ccgctataac ggcgtcgagg ttgaccgctt cggcgcgcag ggtgttcgcg
    11161 gcagtttccg cgcaaagtgg gcgattcctc cggcggacaa acttatctcg tttctcagcc
    11221 gcctaatccc tcgcaaaggc gcagacattt tgatcgaggc ttttgcaaaa gcctgccctc
    11281 acagcggcac attggttatc gccggcccgg aaggccaagc cggttacgta agccatttga
    11341 aaaaatacgc ggaagagaat cgcgtcgcga accgcgtgat tttcacgggc cctgtttacg
    11401 acgcggacaa agccgccttg tattccgatt cggacgtgtt cgtacttgct tcgcgctacg
    11461 agaattttgc gaatgtcgtt gccgaagccg tggcgtgtga tattcctgta attgtttcga
    11521 atatgtgtgg aatccattca ctcgtggacg ggcgtgcagg cattgtcacg tcggttcaac
    11581 gagattcaat cgccgccgcc ttgcagcggt tattagcaga tgaagcgttg tacgctcggc
    11641 tcagagcggg atgtcgagat gtagcacagg aattaacttg gggaactctt agcgctcaga
    11701 tggagaacta ctattcagag gtgcttgctg gcagcaattg atctaaagaa gcggttggtt
    11761 gctccttgtc cactttgccg gatttccaga ggatatccgc ccccgtattg catgcgattg
    11821 gaggtgtaat gaaagcgcgc gcttctgctt cactctctgc acacaactct cacattccag
    11881 gcactgtctc gaacgcggca gcgcgcgacc ttcgcgaggc tggctttggt cacggcatct
    11941 ttcgcgtgac ttggtacggt gtgaacatac tcctgtttgc tgcgcttgtg ggtgtcttgt
    12001 ggacagccgc atgggaatat tccaccgaat gctacttgag agggttttcg gacgcggtca
    12061 tccctttttc cgcggctcca actcggaaaa ttgaagcgat cctgcattgg atgggacact
    12121 ctccggcgcg cttcgatcaa gctcccgagt caatggcgga tgatcgagat ccgaccgaca
    12181 cgttgaatta cgcctcgctt ctcagcgtct gtggtactgc cacaaatgcg ttcttaaatc
    12241 ttgctgatac cggcggctta actgtgcgga ggctcttact gctcgatgtg aatcgcacga
    12301 cggtccacgt tgtagccgaa gtcttgattg acgggagatg gattattgtc gatcccgtgt
    12361 tccatacgat ttttcgcggt accgatggtg gaatgttgac gagccagcaa ctcgcatctc
    12421 cgatcgtttt ccgtagggcc actcagaata ttcccggtta cctgcccacg tatacgtttg
    12481 atcgtgccgt tcacgtgcgc ttatctcatt ttggaccgct tggccgatgg gtgcgaaaca
    12541 tatggggagt tccagcgtgg gagggctggc cggtactgag tctgattatg gagcgcgaat
    12601 cgctggaatt atttgttgtg tttgccttct tggtcttgtg cttactgatt gtgcgcggcg
    12661 tgctccgctg gtacggcgag gcccggctcg gcattcgcat ggagcacatg tatcggaagt
    12721 tattccgagc gggccggttg ctcttcgaac ctaccgatta gccagggcta gtcgaccgcg
    12781 actcgcgtgc cgcgactctc cgccttggac atgcgatcga tgcgctcgtc gatcacgctg
    12841 cggactgcct tgaggaattc aatccgcgaa ttcttcaagt gctgacgcac cgtgggcgat
    12901 acgccaaaaa gatcctgcac atggccgaac agctcctcgc acacgcactg atgtccgcgc
    12961 tctcctgcag cttgttgatt cgcttgctcg ttcattgcgc ctccttcatt tccaccgtaa
    13021 gaacacgatg ctcaagccgt gcccggccgg gattcagcat tgccatcgac cgcggcaggc
    13081 cgatgtgcct ccgcaacgtg ccaatctcga tcactagctc gtcgcctttt ttgaaaagcc
    13141 cgacgtcgcc tttggccgcg aacgggagca gaatccggac ctggtaatgg ccatccgact
    13201 tctcgaaagt atacggcttt tcggttcgcg ttacagccgc agggtcctca ccggaaggat
    13261 acagcaaact ggcgagccgt tcgaggcgtt ccttgccgag cacttcctgt tcgaagagag
    13321 gaacacgctt caccggcaca ggcgcgaaat attcctcgat ttcacgcaaa atgccttcct
    13381 gcgacttgtg ccacgcggtg aaccatgcgt ccgtaacatc cgaaggcaag agcctattca
    13441 ccacgacggt atcgaccgtc agtccatgca gagagaagta gacgaacgcg cgctccgtct
    13501 cgcgtagcac catcttttca ggattagtca cgaggcgcac gcttgttgtc ttcggatctt
    13561 ccatcagatc gtccacgcct tcgagctttt cgaagagatt ccttatgtta acgaaatagt
    13621 tatctgtcgg caactcgaat ggggctacac gattcgcaat cggccgcacg gctttcagca
    13681 gatttcgctg gaacggaaaa atgtgcttca tgtaccagtc gagcgtcgtc ggcatgctga
    13741 tgaagcgcat cgattcggcg gttggtgccg cgtccagaac aatcgcgtca taggtctgct
    13801 cgcgccggta ttgattgatg tacatcatgg cgctgagctc ttccatgccg ggcaggatcg
    13861 caagctcctc cgcctccacg ccgctgatcc ctgttgtgcg cagtacggac gtgatgtacg
    13921 acgagatctc ctgccagtgc cgcttgatct ctttttgaat attcagctcg aaaatcgaga
    13981 gacgattcgt gatcggcagg ggatcggacg tttgcgcgtg aaaaagatcg gtattcaggt
    14041 cgaatgaatc cgccaagctg tgcgccggat cgatactcat caccagcgtg cggtaattca
    14101 gttccgccag cttcagtccc gtggccgcgg caaggctcgt cttgccgacc ccgcccttcc
    14161 cgctaaacaa tagaattctc atagcacttc cattatagaa ggtttcggaa gcggcggcgt
    14221 ctcagctctc gtttgcctcc tgaatacgtt cgctttttcc ctccggcaat tgcgattaga
    14281 agtgggtacc tccttttgtt ttcatggagt tacgtccagt tttaattctt gtgagttgcc
    14341 ccgccaaaat ggcggcgggc ggggattagg aagacaggtg acaggttttc agcttgtagc
    14401 tgacaggtga tcgaaaaatg cccttttttt gtcgattaga aattggattt ttgttgagag
    14461 cacagaagtt acgtcgattt ctaatcggca gattggcgat tagaaaatcc gtgggtggtg
    14521 ggtggaaatt ggtgaatggt ggggatgccg tccttcgcgc cttccgctcg ttcgctccga
    14581 ttgttaccat ggtcgccatg acataaaata cggaccacag tttaccacac gttcaagata
    14641 cttgcaagga tttttaatcg tcaatttggg gcaactgacc cgcgcaatat gggacaaatt
    14701 tggcgcgatt ggtaccttaa aatgggctta agtggt
//
LOCUS       DATNDT010000536         5172 bp    DNA     linear   ENV 15-FEB-2024
DEFINITION  MAG TPA_asm: Verrucomicrobiia bacterium isolate SMAG_U11249
            k141_1108527, whole genome shotgun sequence.
ACCESSION   DATNDT010000536 DATNDT010000000
VERSION     DATNDT010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37945822
            Sequence Read Archive: SRR6185437
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Verrucomicrobiia bacterium (soil metagenome)
  ORGANISM  Verrucomicrobiia bacterium
            Bacteria; Verrucomicrobiota; Verrucomicrobiia.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/06/2024 01:23:29
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,509
            CDSs (total)                      :: 3,471
            Genes (coding)                    :: 3,446
            CDSs (with protein)               :: 3,446
            Genes (RNA)                       :: 38
            rRNAs                             :: 1, 2 (16S, 23S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 34
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 25
            CDSs (without protein)            :: 25
            Pseudo Genes (ambiguous residues) :: 0 of 25
            Pseudo Genes (frameshifted)       :: 12 of 25
            Pseudo Genes (incomplete)         :: 18 of 25
            Pseudo Genes (internal stop)      :: 1 of 25
            Pseudo Genes (multiple problems)  :: 5 of 25
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Verrucomicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1108527"
                     /isolate="SMAG_U11249"
                     /isolation_source="soil"
                     /db_xref="taxon:2499141"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="42.39 N 85.37 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VN788_17510"
     CDS             complement(<1..342)
                     /locus_tag="VN788_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HXU22380.1"
                     /translation="MSLNQPSRVDLLQGTLDLLILRTLLSGRAHGHAIAKYIQRTSED
                     LLQVETGSLYPALHRLEAKGWIAASWELSDKGKRARYYRLTAAGRKQLGIEQSKWRAL
                     SRAIGLILNPVD"
     gene            495..1721
                     /locus_tag="VN788_17515"
     CDS             495..1721
                     /locus_tag="VN788_17515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22381.1"
                     /translation="MKSTLLTLLLLAPFASTQAAEPPAPAPQDFAIMAWGQSPSDPGQ
                     LRGMRDAGLNISGFCHAGDLDKVRAAGLTCFVSDPGIDSVDLFHLPPDSELRTRVEAL
                     KKQIGGNPAALGFFLRDEPDAPSMPALGKLASLLREAMPDKWPYVNLFPSRVSAQRLG
                     VADYDSYVRMLVKTVGQPFLSYDNYSLVNGEMLDYFYTNLDIVRRLGIETHTPFWNCI
                     LANSHFNYMEPSDATFNLQVYSTLAYGGRGIQYFTYFAPEIGNYRMAAVDQFGNRTPT
                     WDLLRRINNQIHALAPTMIRLHSTGVYHYPDVPDQGQPLSASKLIESVEMTQHVRPPV
                     AVGRFLVGEFEDAEGRPYFMIVNKDLKNSFQFKVHLKKEGGKLIRISPYTGQEEPFGR
                     EMDWLAPGAGVLLRIE"
     gene            complement(1944..2231)
                     /locus_tag="VN788_17520"
     CDS             complement(1944..2231)
                     /locus_tag="VN788_17520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22382.1"
                     /translation="MSASCPDSAAIHGSEGDLVLISVSVDPKLLEDFLDALAGLDFPV
                     NPQLYHHPGSVAVEFPAYKSRVDEVRETLVRRGFDAAGVRVAAMLEAIAGR"
     gene            complement(2486..2863)
                     /locus_tag="VN788_17525"
     CDS             complement(2486..2863)
                     /locus_tag="VN788_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22383.1"
                     /translation="MSSGDQPSWRDRRILTTLLLVFLAGGALGAVTMRAYVHQRLHHA
                     IAYWKYPDIPVSYDRMKDELQLTPAQCGQLKSILDDMVSYRRDIEAQVYSFQATGKYR
                     IMAILTPGQQKRFEKISKEMIGR"
     gene            complement(2867..3346)
                     /locus_tag="VN788_17530"
     CDS             complement(2867..3346)
                     /locus_tag="VN788_17530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HXU22384.1"
                     /translation="MHQPIRDGLEDYLNGNTPSGEFREHLAACESCAARVRELERHSR
                     WIQTLRHQAEIDPAAGFYARVLERIERRTGNSIWSVFLQPGFSRRIAYASMAMVLLLG
                     TYIVTTEPGESVFGSSPVAAQMSYSSPRAMELSATDAATPQDRDAVLVNLVSYQDAQ"
     gene            complement(3474..4139)
                     /locus_tag="VN788_17535"
     CDS             complement(3474..4139)
                     /locus_tag="VN788_17535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HXU22385.1"
                     /translation="MGSLTGVLPFPASGEKGRTKTRVDHRNSEEAVLVRRVQARDEIA
                     FREIVERYQAKVFSIIYGILRNHNDAEDIAQQVFAKIYFSIKNFDFRSSLLTWIYKIT
                     VNECYDYLRKKRVRKLVYESDFSEEDARRMENSDTASDHAPAVDVELAQRDLIVKLLD
                     KLSDDDRSLIVMKEVEGHSVEELSRMTGMNENTIKVKLFRARQKLLKAAQRLERSGGK
                     KLM"
     gene            4362..4685
                     /locus_tag="VN788_17540"
     CDS             4362..4685
                     /locus_tag="VN788_17540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HXU22386.1"
                     /translation="MVQLTERAVGKVREILETQEPKPAGLRIAVVGGGCSGFSYSMAF
                     ENTPGFLDKTYKFDDLKVFVDQASMLYLDGVEVDYLETLEGSGFKFNNPNVKSTCGCG
                     SSFNA"
     gene            4765..>5172
                     /locus_tag="VN788_17545"
     CDS             4765..>5172
                     /locus_tag="VN788_17545"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HXU22387.1"
                     /translation="MLEQLLTEQPNPASRNIDALPTGQILEIINQEDKKIAVAVEVEI
                     PNIARAVDAIVAALRAGGRLFYIGAGTSGRLGVLDASECPPTFDVPPEMVQGIIAGGE
                     RALARSTEASEDDPASGERDLLERGFTGKDILAG"
BASE COUNT         1078 a         1511 c         1461 g         1122 t
ORIGIN      
        1 atccaccgga ttcaagatca agccgatcgc gcgggagagc gcccgccatt tcgattgttc
       61 gataccgagt tgtttccggc cggccgccgt gaggcgatag tacctggcgc gcttgccttt
      121 gtcggagagt tcccaggaag ccgcgatcca gcctttcgct tcgagacgat ggagagccgg
      181 gtacaaagaa ccggtctcga cctggagcaa gtcctccgag gtccgttgaa tgtatttcgc
      241 gatggcgtgg ccgtgcgcgc gtccgctcag cagggtgcgg aggatgagga gatcgagtgt
      301 gccctgcaga agatccacgc gcgagggctg attgaggctc attgtgtagc cagtctacta
      361 tcttttatgt agagtgtcta ttacattatt ttcgtgcttc aactaccgat attgccctct
      421 tcggtttatc tcgcgccgac gctctcttga agacccgatc cgcgttcagt acaatggcgg
      481 ccacgactac gccgatgaaa agcacattgc tcaccttgct cctgctcgcg ccttttgcca
      541 gcacccaggc cgcggagccg ccggcgcccg ctcctcagga tttcgccatc atggcttggg
      601 ggcagtctcc ctccgacccc ggccagctac gcgggatgcg cgacgcggga ttgaacattt
      661 ccggcttctg ccacgctggg gatctcgaca aggtccgcgc cgccgggctg acttgctttg
      721 tgagcgatcc gggtattgac agcgtggacc tgttccacct tccgccggac agcgaactgc
      781 gtacccgcgt ggaggcgctg aagaagcaga tcggcggaaa tccggccgcg ctcgggtttt
      841 ttctacgcga cgagcctgat gcccctagca tgcccgcgct cggcaaactt gcctctctgt
      901 tgcgcgaggc aatgccggac aaatggcctt acgtgaacct gtttccgtcg cgcgtttcgg
      961 cacagcggct cggcgtcgcg gattatgact cgtacgtccg gatgctggtg aagaccgttg
     1021 ggcaaccgtt cctcagttac gacaactatt cgctggttaa cggggagatg ctcgactatt
     1081 tttataccaa cctcgatatc gtgcggcgcc tcggtataga aacacacacg cccttctgga
     1141 actgcattct cgcgaactcc cacttcaact acatggagcc ctccgacgcc accttcaacc
     1201 tgcaggtcta cagcactcta gcctacgggg gccgcggcat ccagtacttc acttacttcg
     1261 cgccggagat cggcaattac cggatggcgg ccgtcgatca gttcgggaac cgcacgccca
     1321 catgggacct gctccggcgg atcaacaatc agattcacgc gctcgcgccg acgatgattc
     1381 gcctgcacag caccggcgtt tatcattacc cggatgttcc ggatcagggc cagccgctgt
     1441 cggcgagcaa gctgattgag tccgtggaga tgacgcagca tgtccggcca cctgttgcgg
     1501 tgggacgctt cctggtggga gagtttgagg atgccgaggg acggccgtat ttcatgatcg
     1561 tgaacaagga cctgaagaat tcgtttcagt tcaaggttca tctgaagaag gaaggcggga
     1621 agttgattcg catctcgccg tatactggcc aggaagaacc gtttggccgc gaaatggact
     1681 ggcttgcgcc gggcgcggga gtgcttctac ggatcgaata aggacggtga gacggagcga
     1741 tttgggtcgc tcgatctgtc cggagtgcag gacctcgtac caagtcatgg aaatccggtt
     1801 gcagtaccga tgaaccgctt gtaacgcgcg cggctcagca ccgcggttca gatccacgta
     1861 gagctaaact ccgatgcttg gttacctccg ccctgcgcgg ccgaattttt ggcttcgccg
     1921 ggacgaaggt gaagcgaatg gtatcaccgc ccggcgatcg cttcgagcat cgccgcgaca
     1981 cggacccccg ctgcgtcaaa accccggcgg accaacgttt cccgtacttc atccaccctg
     2041 gatttatacg cggggaattc caccgcgacc gatccaggat ggtggtaaag ctgagggttg
     2101 actgggaaat cgagaccggc aagagcgtcg agaaaatctt cgagtaactt gggatcgacc
     2161 gagacggaaa tcaggaccag atcgccttcg gagccgtgta ttgcggcgga atccggacag
     2221 gaagcgctca tgaggaactc ctctccttag agcgacccgg tccgccaata aaaaacccct
     2281 gtgcgattgg cccaggggat ttagactcgt gaagaccgac tcgcgtttag cactttttag
     2341 cgtggttgca attagccgat accgttctca cggcatctaa atcaatccac gtttcgatta
     2401 ctaatttagc acttacccgg tccggttgca acgaaaaagt aaaaacgagg taaattgcac
     2461 cgctcgattc ccgatcgcgt cccgctcaac gcccgatcat ctccttcgag atcttttcga
     2521 agcgcttctg ttgaccgggg gtaaggatcg ccataatgcg gtacttgccg gtggcctgga
     2581 acgaatagac ttgagcttca atatcccgcc gatagctcac catgtcgtcc agaatcgatt
     2641 tcaattggcc acattgagcc ggcgtgagtt ggagttcgtc tttcatgcgg tcgtaggaga
     2701 ccgggatgtc tggatatttc caatacgcga tcgcgtggtg aagccgctga tgcacgtatg
     2761 cccgcatcgt caccgctccg agcgcgccgc ccgccaggaa caccaaaagc agtgtggtca
     2821 gaatccggcg gtcacgccag gagggctggt cccctgagga catagtctat tgcgcgtcct
     2881 gataggaaac cagattcact agcaccgcgt cgcggtcctg tggggtggcc gcatcggttg
     2941 cggataactc catcgcacga ggcgaactgt aactcatctg cgccgccacc ggggatgaac
     3001 cgaaaaccga ttcgcccggc tccgtagtca caatgtaagt cccgagcagc agaaccattg
     3061 ccatggaggc gtacgcaatc cgtctactga agcccggctg gagaaacacg gaccagatcg
     3121 aatttcccgt ccgccgctca attctttcta gaacgcgggc gtaaaagccc gccgctggat
     3181 cgatctccgc ctggtgacgc agagtttgaa tccaccgcga atgccgttcg agttcccgca
     3241 cgcgcgccgc gcagctctcg caagcggcca aatgctcccg gaactcaccg gacggcgtgt
     3301 tgccgttgag gtaatcctct aggccatccc gtatcggctg gtgcataaat ccattccctc
     3361 aaaaagttca ttccatccgg cgctcggatc taatgcccgt ttgcgcctcc ggtccggcct
     3421 ctttgcgccg cacctttcca tattcaaatc gaccacttca catcccggcg ccctcacatc
     3481 aatttcttac cgccggagcg ctccaacctc tgggccgcct tcaacagctt ctggcgagcc
     3541 cggaacagtt tgacctttat tgtattctca ttcatcccgg tcatacggga caattcttcc
     3601 actgagtgcc cttccacttc cttcataacg atgagagacc ggtcgtcgtc ggatagctta
     3661 tcaagcagct tcacaatcag atcgcgctgc gcgagctcga catcgacagc cggcgcgtgg
     3721 tcggaggcgg tgtcgctgtt ttccatcctg cgagcgtctt cctcgctgaa atcgctctca
     3781 taaacgagtt tacgaactcg ctttttgcgc agataatcgt agcactcatt gaccgtgatc
     3841 ttatagatcc aagtcagcag cgaactgcgg aaatcgaaat ttttgatcga aaagtagatt
     3901 ttcgcgaaga cctgctgagc gatatcctcg gcatcattat gattgcggag gattccatag
     3961 atgatggaaa agaccttcgc ctggtagcgc tcgacgatct cacggaaggc gatttcgtcc
     4021 ctggcctgca cacggcggac caggaccgct tcttcactat tccgatgatc caccctggtt
     4081 tttgtccggc ccttttcgcc ggaagccggg aacggaagta cgccagtcag gctgcccata
     4141 cgctccagga gacgcggccc atggccctcg ggttccaggc gcttctaact ggcacatcgc
     4201 cctccatttg agcggcatcc tatgggcgag tttagcatcg aaataggtaa gtgtctctcg
     4261 gctgcggaag ggcgtcgatg cgaattgagt gcgacttgat tccggcgtgg acagacacga
     4321 taatggaaat gccggtacca aacacaagga gaacaacgac aatggtgcaa ctgactgaac
     4381 gggcagtggg taaggtgaga gagattctgg aaacccagga accgaagccg gcaggtcttc
     4441 ggatcgccgt ggtcggcggc ggctgctcgg ggtttagcta ctccatggcc ttcgaaaata
     4501 ccccagggtt tcttgacaag acttacaagt tcgacgatct gaaggttttc gtcgatcagg
     4561 cgagcatgct ttacctggat ggcgtcgaag tcgattatct ggagacgctc gaaggtagcg
     4621 gattcaagtt caacaatccc aacgtgaaga gcacttgcgg ttgcggcagc tcctttaacg
     4681 cttagaagac ggcgaagttc aggatcggga gcgcctaccg ggaaatctca tggcgctctc
     4741 cttcgttttt ggattctcgc ctgcatgctt gaacaactcc tcaccgaaca gcctaacccc
     4801 gcatcccgta atatagacgc cctacctacc gggcagatcc ttgagattat taatcaggaa
     4861 gacaagaaga ttgccgtggc cgtcgaggtg gagatcccga acattgctcg cgccgtagat
     4921 gctattgtcg cggctctgcg ggccggtgga cggcttttct acattggcgc ggggaccagc
     4981 gggcgcctag gcgttctgga tgcctcggaa tgcccgccga ccttcgacgt gcctccggaa
     5041 atggtgcaag gcatcatcgc cggtggggaa cgcgctctcg cgcgatcgac ggaggcgtct
     5101 gaagacgatc cggctagtgg tgagcgcgat ctgctggaac gcggtttcac cggcaaagat
     5161 atcctcgctg gc
//
